@17:22:17.07PDT24-Jul-18
>BREAK< ESP3 Gateway v0.98 for core bus @17:22:17.10 >BREAK< ESP3 Gateway v0.98 for cartridge bus @17:22:18.66 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @17:22:20.14 Polling Can every 5:00 @17:22:20.24 Gate.power :analytic1,:ON,limit to 5000mA @17:22:20.26 Gate.power :analytic2,:ON,limit to 5000mA @17:22:20.27 Gate.power :analytic3,:ON,limit to 5000mA @17:22:20.96 ArS.configure ArSconfig @17:22:21.12 SPR Module 1, FW Rev. B, FPGA Rev. C @17:22:21.14
LyS1.configure LyS1config @17:22:21.27 LyS2.configure LyS2config @17:22:21.38 AirS.configure AirSconfig @17:22:21.52 TV.configure TVconfig @17:22:21.67 SP.configure SPconfig @17:22:21.80 SC.reconfigure SCconfig @17:22:21.85 SC.hold @17:22:21.92 SC.configure SCconfig @17:22:22.07 PV.configure PVconfig @17:22:22.20 PinPort.configure PPconfig @17:22:22.33 Sea.configure SPconfig @17:22:22.38 All elf controllers running firmware version 4.46 @17:22:22.46 Intake.close @17:22:22.50 Exhaust.close @17:22:22.58 PB.close @17:22:22.81
Loading /home/esp/esp2/mission/processAll.rb mission @17:22:22.85 Processing all remaining dry cartridges in: {dry: [1, 2, 8, 9, 13, 18, 23, 28, 29, 32, 34, 37, 40, 43, 46, 49, 52, 55, 56]} @17:22:22.87 Sampling maximum of 500.0ml per cartridge @17:22:22.88 Fixing samples for 10:00, evacuating once @17:22:22.89 delay 30 seconds @17:22:53.65 SC.seek 52 @17:22:53.73 Ensuring all (60) cartridges are bypassed @17:22:54.11 SC.seek 1 @17:23:04.76 TV.seek :bypass @17:23:06.37 TV.seek :clear @17:23:08.35 SC.seek 2 @17:23:08.81 TV.seek :bypass @17:23:10.43 TV.seek :clear @17:23:12.39 SC.seek 3 @17:23:12.85 TV.seek :bypass @17:23:14.47 TV.seek :clear @17:23:16.43 SC.seek 4 @17:23:16.88 TV.seek :bypass @17:23:18.50 TV.seek :clear @17:23:20.45 SC.seek 5 @17:23:21.26 TV.seek :bypass @17:23:22.87 TV.seek :clear @17:23:24.85 SC.seek 6 @17:23:25.31 TV.seek :bypass @17:23:26.93 TV.seek :clear @17:23:28.89 SC.seek 7 @17:23:29.35 TV.seek :bypass @17:23:30.97 TV.seek :clear @17:23:32.94 SC.seek 8 @17:23:33.39 TV.seek :bypass @17:23:35.02 TV.seek :clear @17:23:36.99 SC.seek 9 @17:23:37.44 TV.seek :bypass @17:23:39.06 TV.seek :clear @17:23:41.03 SC.seek 10 @17:23:41.48 TV.seek :bypass @17:23:43.10 TV.seek :clear @17:23:45.07 SC.seek 11 @17:23:45.53 TV.seek :bypass @17:23:47.15 TV.seek :clear @17:23:49.13 SC.seek 12 @17:23:49.59 TV.seek :bypass @17:23:51.21 TV.seek :clear @17:23:53.16 SC.seek 13 @17:23:53.62 TV.seek :bypass @17:23:55.23 TV.seek :clear @17:23:57.20 SC.seek 14 @17:23:58.03 TV.seek :bypass @17:23:59.64 TV.seek :clear @17:24:01.62 SC.seek 15 @17:24:02.09 TV.seek :bypass @17:24:03.71 TV.seek :clear @17:24:05.69 SC.seek 16 @17:24:06.14 TV.seek :bypass @17:24:07.76 TV.seek :clear @17:24:09.74 SC.seek 17 @17:24:10.20 TV.seek :bypass @17:24:11.82 TV.seek :clear @17:24:13.82 SC.seek 18 @17:24:14.27 TV.seek :bypass @17:24:15.89 TV.seek :clear @17:24:17.85 SC.seek 19 @17:24:18.31 TV.seek :bypass @17:24:19.92 TV.seek :clear @17:24:21.89 SC.seek 20 @17:24:22.35 TV.seek :bypass @17:24:23.97 TV.seek :clear @17:24:25.94 SC.seek 21 @17:24:26.40 TV.seek :bypass @17:24:28.03 TV.seek :clear @17:24:29.98 SC.seek 22 @17:24:30.43 TV.seek :bypass @17:24:32.05 TV.seek :clear @17:24:34.01 SC.seek 23 @17:24:34.85 TV.seek :bypass @17:24:36.48 TV.seek :clear @17:24:38.45 SC.seek 24 @17:24:38.91 TV.seek :bypass @17:24:40.53 TV.seek :clear @17:24:42.51 SC.seek 25 @17:24:42.99 TV.seek :bypass @17:24:44.60 TV.seek :clear @17:24:46.56 SC.seek 26 @17:24:47.02 TV.seek :bypass @17:24:48.65 TV.seek :clear @17:24:50.62 SC.seek 27 @17:24:51.08 TV.seek :bypass @17:24:52.70 TV.seek :clear @17:24:54.68 SC.seek 28 @17:24:55.15 TV.seek :bypass @17:24:56.76 TV.seek :clear @17:24:58.72 SC.seek 29 @17:24:59.19 TV.seek :bypass @17:25:00.81 TV.seek :clear @17:25:02.76 SC.seek 30 @17:25:03.22 TV.seek :bypass @17:25:04.84 TV.seek :clear @17:25:06.81 SC.seek 31 @17:25:07.27 TV.seek :bypass @17:25:08.89 TV.seek :clear @17:25:11.13 SC.seek 32 @17:25:11.68 TV.seek :bypass @17:25:13.30 TV.seek :clear @17:25:15.28 SC.seek 33 @17:25:15.74 TV.seek :bypass @17:25:17.35 TV.seek :clear @17:25:19.32 SC.seek 34 @17:25:19.76 TV.seek :bypass @17:25:21.39 TV.seek :clear @17:25:23.36 SC.seek 35 @17:25:23.81 TV.seek :bypass @17:25:25.43 TV.seek :clear @17:25:27.40 SC.seek 36 @17:25:27.85 TV.seek :bypass @17:25:29.47 TV.seek :clear @17:25:31.43 SC.seek 37 @17:25:31.88 TV.seek :bypass @17:25:33.51 TV.seek :clear @17:25:35.49 SC.seek 38 @17:25:35.94 TV.seek :bypass @17:25:37.56 TV.seek :clear @17:25:39.53 SC.seek 39 @17:25:40.00 TV.seek :bypass @17:25:41.62 TV.seek :clear @17:25:43.60 SC.seek 40 @17:25:44.06 TV.seek :bypass @17:25:45.68 TV.seek :clear @17:25:48.01 SC.seek 41 @17:25:48.47 TV.seek :bypass @17:25:50.09 TV.seek :clear @17:25:52.07 SC.seek 42 @17:25:52.53 TV.seek :bypass @17:25:54.16 TV.seek :clear @17:25:56.14 SC.seek 43 @17:25:56.60 TV.seek :bypass @17:25:58.22 TV.seek :clear @17:26:00.19 SC.seek 44 @17:26:00.66 TV.seek :bypass @17:26:02.28 TV.seek :clear @17:26:04.23 SC.seek 45 @17:26:04.69 TV.seek :bypass @17:26:06.30 TV.seek :clear @17:26:08.27 SC.seek 46 @17:26:08.72 TV.seek :bypass @17:26:10.34 TV.seek :clear @17:26:12.31 SC.seek 47 @17:26:12.77 TV.seek :bypass @17:26:14.39 TV.seek :clear @17:26:16.36 SC.seek 48 @17:26:16.82 TV.seek :bypass @17:26:18.43 TV.seek :clear @17:26:20.40 SC.seek 49 @17:26:20.86 TV.seek :bypass @17:26:22.48 TV.seek :clear @17:26:24.82 SC.seek 50 @17:26:25.28 TV.seek :bypass @17:26:26.91 TV.seek :clear @17:26:28.88 SC.seek 51 @17:26:29.35 TV.seek :bypass @17:26:30.97 TV.seek :clear @17:26:32.94 SC.seek 52 @17:26:33.39 TV.seek :bypass @17:26:35.01 TV.seek :clear @17:26:36.99 SC.seek 53 @17:26:37.45 TV.seek :bypass @17:26:39.07 TV.seek :clear @17:26:41.04 SC.seek 54 @17:26:41.50 TV.seek :bypass @17:26:43.12 TV.seek :clear @17:26:45.10 SC.seek 55 @17:26:45.56 TV.seek :bypass @17:26:47.18 TV.seek :clear @17:26:49.16 SC.seek 56 @17:26:49.61 TV.seek :bypass @17:26:51.23 TV.seek :clear @17:26:53.19 SC.seek 57 @17:26:53.65 TV.seek :bypass @17:26:55.27 TV.seek :clear @17:26:57.25 SC.seek 58 @17:26:57.71 TV.seek :bypass @17:26:59.33 TV.seek :clear @17:27:01.67 SC.seek 59 @17:27:02.13 TV.seek :bypass @17:27:03.75 TV.seek :clear @17:27:05.73 SC.seek 60 @17:27:06.19 TV.seek :bypass @17:27:07.81 TV.seek :clear @17:27:09.43 Selecting Cartridge 56 @17:27:09.81 SC.seek 56 @17:27:10.97 LyS2.seek :locked @17:27:11.12 PV.seek :locked @17:27:11.26 AirS.seek :locked @17:27:15.35
Powering cartridge bus @17:27:21.48 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @17:27:21.49 Retry #1 of 2 @17:27:21.50 LyS2.coast @17:27:21.55 AirS.coast @17:27:21.59 TV.coast @17:27:21.64 SC.coast @17:27:21.68 PV.coast @17:27:21.85 Gate.power :motor,:OFF @17:27:21.87 Gate.power :main,:OFF @17:27:21.88 Gate.power :cartridge,:OFF @17:27:21.89 Gate.power :core,:OFF @17:27:21.91 Gate.power :analytic1,:OFF @17:27:21.92 Gate.power :analytic2,:OFF @17:27:21.93 Gate.power :analytic3,:OFF @17:27:22.05 Gate.power :main,:ON,limit to 5000mA @17:27:22.06 Gate.power :analytic1,:ON,limit to 5000mA @17:27:22.08 Gate.power :analytic2,:ON,limit to 5000mA @17:27:22.09 Gate.power :analytic3,:ON,limit to 5000mA @17:27:22.94 Powering core bus @17:27:24.53 SPR Module 1, FW Rev. B, FPGA Rev. C @17:27:25.24
ArS.configure ArSconfig @17:27:25.35 LyS1.configure LyS1config @17:27:25.49 LyS2.configure LyS2config @17:27:25.61 AirS.configure AirSconfig @17:27:25.74 TV.configure TVconfig @17:27:25.88 SP.configure SPconfig @17:27:26.02 SC.configure SCconfig @17:27:26.16 PV.configure PVconfig @17:27:26.30 PinPort.configure PPconfig @17:27:26.44 Sea.configure SPconfig @17:27:26.49 All elf controllers running firmware version 4.46 @17:27:26.51 Gate.power :motor,:ON,limit to 5000mA @17:27:27.15 PV.reconfigure PVconfig @17:27:27.20 TV.reconfigure TVconfig @17:27:27.23 AirS.reconfigure AirSconfig @17:27:27.27 LyS1.reconfigure LyS1config @17:27:27.33 ArS.reconfigure ArSconfig @17:27:27.38 LyS2.reconfigure LyS2config @17:27:27.42 PinPort.reconfigure PPconfig @17:27:27.71 TV.home.jog 0 @17:27:27.74 AirS.home.jog 0 @17:27:27.86 LyS1.home.jog 0 @17:27:27.96 PinPort.home.jog 0 @17:27:28.24 PV.home.jog 0 @17:27:28.28 ArS.home.jog 0 @17:27:28.34 LyS2.home.jog 0 @17:27:29.56 PV.reconfigure HomePV @17:27:29.62 AirS.reconfigure HomeMagSyringe @17:27:29.67 ArS.reconfigure HomeMagSyringe @17:27:29.72 PinPort.reconfigure HomePP @17:27:29.75 TV.reconfigure HomeTV @17:27:29.80 LyS1.reconfigure HomeSyringe @17:27:30.04 PV.jog -1487 @17:27:30.09 AirS.jog -2047 @17:27:30.13 PinPort.jog -109 @17:27:30.18 ArS.jog -275 @17:27:30.22 TV.jog -555 @17:27:30.30 LyS1.jog -274 @17:27:30.58 LyS2.reconfigure HomeSyringe @17:27:30.65 LyS2.jog -1438 @17:27:30.74 PinPort.setRawPosition! 0 @17:27:30.79 PinPort.seek :home @17:27:30.89 PinPort.reconfigure PPconfig @17:27:30.99 ArS.setRawPosition! -50 @17:27:31.05 ArS.seek :home @17:27:31.14 LyS1.setRawPosition! -50 @17:27:31.19 LyS1.seek :home @17:27:31.35 ArS.reconfigure ArSconfig @17:27:31.46 PV.setRawPosition! -50 @17:27:31.51 PV.seek :home @17:27:31.61 LyS1.reconfigure LyS1config @17:27:31.75 AirS.setRawPosition! -50 @17:27:31.83 AirS.seek :home @17:27:31.94 LyS2.setRawPosition! -50 @17:27:31.96 PV.reconfigure PVconfig @17:27:32.05 LyS2.seek :home @17:27:32.19 AirS.reconfigure AirSconfig @17:27:32.36 TV.setRawPosition! 0 @17:27:32.42 TV.seek :home @17:27:32.53 LyS2.reconfigure LyS2config @17:27:32.57 TV.reconfigure TVconfig @17:27:33.22
TV.seek :clear @17:27:34.84 TV.coast @17:27:34.90 SC.reconfigure HomeSC @17:27:34.96 SC.home.jog 0 @17:27:35.73 SC.reconfigure SCconfig @17:27:35.78 SC.seek 56 @17:27:35.87 Selecting Cartridge 56 @17:27:36.14 LyS2.seek :locked @17:27:36.31 PV.seek :locked @17:27:36.48 AirS.seek :locked @17:27:40.58
Powering cartridge bus @17:27:46.71 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @17:27:46.72 Retry #2 of 2 @17:27:46.73 ArS.coast @17:27:46.77 LyS1.coast @17:27:46.82 LyS2.coast @17:27:46.86 AirS.coast @17:27:46.90 SC.coast @17:27:46.93 PV.coast @17:27:46.98 PinPort.coast @17:27:47.16 Gate.power :motor,:OFF @17:27:47.18 Gate.power :main,:OFF @17:27:47.19 Gate.power :cartridge,:OFF @17:27:47.20 Gate.power :core,:OFF @17:27:47.22 Gate.power :analytic1,:OFF @17:27:47.23 Gate.power :analytic2,:OFF @17:27:47.24 Gate.power :analytic3,:OFF @17:27:47.69 Gate.power :main,:ON,limit to 5000mA @17:27:47.72 Gate.power :analytic1,:ON,limit to 5000mA @17:27:47.73 Gate.power :analytic2,:ON,limit to 5000mA @17:27:47.74 Gate.power :analytic3,:ON,limit to 5000mA @17:27:48.25 Powering core bus @17:27:50.19 SPR Module 1, FW Rev. B, FPGA Rev. C @17:27:50.57
ArS.configure ArSconfig @17:27:50.68 LyS1.configure LyS1config @17:27:50.83 LyS2.configure LyS2config @17:27:50.96 AirS.configure AirSconfig @17:27:51.09 TV.configure TVconfig @17:27:51.23 SP.configure SPconfig @17:27:51.37 SC.configure SCconfig @17:27:51.51 PV.configure PVconfig @17:27:51.65 PinPort.configure PPconfig @17:27:51.82 Sea.configure SPconfig @17:27:51.88 All elf controllers running firmware version 4.46 @17:27:51.91 Gate.power :motor,:ON,limit to 5000mA @17:27:52.15 ArS.reconfigure ArSconfig @17:27:52.56 ArS.home.jog 0 @17:27:52.69 LyS1.reconfigure LyS1config @17:27:52.72 PV.reconfigure PVconfig @17:27:52.75 AirS.reconfigure AirSconfig @17:27:52.83 PinPort.reconfigure PPconfig @17:27:52.86 LyS2.reconfigure LyS2config @17:27:52.90 TV.reconfigure TVconfig @17:27:53.16 PV.home.jog 0 @17:27:53.21 LyS1.home.jog 0 @17:27:53.25 AirS.home.jog 0 @17:27:53.28 LyS2.home.jog 0 @17:27:53.31 PinPort.home.jog 0 @17:27:53.35 TV.home.jog 0 @17:27:54.37 PinPort.reconfigure HomePP @17:27:54.46 TV.reconfigure HomeTV @17:27:54.50 LyS2.reconfigure HomeSyringe @17:27:54.55 ArS.reconfigure HomeMagSyringe @17:27:54.59 PV.reconfigure HomePV @17:27:54.63 AirS.reconfigure HomeMagSyringe @17:27:54.72 LyS1.reconfigure HomeSyringe @17:27:54.89 PinPort.jog -108 @17:27:55.01 TV.jog -548 @17:27:55.07 LyS2.jog -1441 @17:27:55.11 ArS.jog -275 @17:27:55.13 AirS.jog -2054 @17:27:55.17 PV.jog -1487 @17:27:55.26 LyS1.jog -274 @17:27:55.64 PinPort.setRawPosition! 0 @17:27:55.68 PinPort.seek :home @17:27:55.78 PinPort.reconfigure PPconfig @17:27:55.99 ArS.setRawPosition! -50 @17:27:56.06 ArS.seek :home @17:27:56.15 LyS1.setRawPosition! -50 @17:27:56.20 LyS1.seek :home @17:27:56.35 ArS.reconfigure ArSconfig @17:27:56.49 LyS1.reconfigure LyS1config @17:27:56.61 LyS2.setRawPosition! -50 @17:27:56.68 LyS2.seek :home @17:27:57.15 AirS.setRawPosition! -50 @17:27:57.18 PV.setRawPosition! -50 @17:27:57.26 AirS.seek :home @17:27:57.35 PV.seek :home @17:27:57.55 TV.setRawPosition! 0 @17:27:57.58 LyS2.reconfigure LyS2config @17:27:57.68 TV.seek :home @17:27:57.88 TV.reconfigure TVconfig @17:27:57.91 AirS.reconfigure AirSconfig @17:27:57.95 PV.reconfigure PVconfig @17:27:58.58
TV.seek :clear @17:28:00.20 TV.coast @17:28:00.26 SC.reconfigure HomeSC @17:28:00.32 SC.home.jog 0 @17:28:01.09 SC.reconfigure SCconfig @17:28:01.15 SC.seek 56 @17:28:01.24 Selecting Cartridge 56 @17:28:01.50 LyS2.seek :locked @17:28:01.66 PV.seek :locked @17:28:01.83 AirS.seek :locked @17:28:05.92
Powering cartridge bus @17:28:12.07 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @17:28:12.20 Schedule::Stop in MAIN_scheduler -- Schedule::Stop @20:11:31.52PDT24-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @20:11:31.56 >BREAK< ESP3 Gateway v0.99 for cartridge bus @20:11:33.05 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @20:11:34.57 Polling Can every 5:00 @20:11:34.62 Loading /home/esp/esp2/mission/processAll.rb mission @20:11:34.66 Processing all remaining dry cartridges in: {dry: [1, 2, 8, 9, 13, 18, 23, 28, 29, 32, 34, 37, 40, 43, 46, 49, 52, 55, 56]} @20:11:34.68 Sampling maximum of 500.0ml per cartridge @20:11:34.69 Fixing samples for 10:00, evacuating once @20:11:34.71 delay 30 seconds @20:12:04.83 Gate.power :main,:ON,limit to 5000mA @20:12:04.84 Gate.power :analytic1,:ON,limit to 5000mA @20:12:04.85 Gate.power :analytic2,:ON,limit to 5000mA @20:12:04.86 Gate.power :analytic3,:ON,limit to 5000mA @20:12:05.00 Powering core bus @20:12:07.31 SPR Module 1, FW Rev. B, FPGA Rev. C @20:12:07.46
ArS.configure ArSconfig @20:12:07.57 LyS1.configure LyS1config @20:12:07.71 LyS2.configure LyS2config @20:12:07.82 AirS.configure AirSconfig @20:12:07.95 TV.configure TVconfig @20:12:08.09 SP.configure SPconfig @20:12:08.23 SC.configure SCconfig @20:12:08.37 PV.configure PVconfig @20:12:08.51 PinPort.configure PPconfig @20:12:08.64 Sea.configure SPconfig @20:12:08.70 All elf controllers running firmware version 4.46 @20:12:08.73 Gate.power :motor,:ON,limit to 5000mA @20:12:08.89 ArS.reconfigure ArSconfig @20:12:09.31 ArS.home.jog 0 @20:12:09.52 PV.reconfigure PVconfig @20:12:09.56 TV.reconfigure TVconfig @20:12:09.60 LyS2.reconfigure LyS2config @20:12:09.68 LyS1.reconfigure LyS1config @20:12:09.73 AirS.reconfigure AirSconfig @20:12:09.79 PinPort.reconfigure PPconfig @20:12:09.98 PV.home.jog 0 @20:12:10.04 TV.home.jog 0 @20:12:10.08 LyS2.home.jog 0 @20:12:10.11 LyS1.home.jog 0 @20:12:10.15 AirS.home.jog 0 @20:12:10.18 PinPort.home.jog 0 @20:12:10.77 ArS.reconfigure HomeMagSyringe @20:12:11.03 TV.reconfigure HomeTV @20:12:11.13 ArS.jog -274 @20:12:11.17 PV.reconfigure HomePV @20:12:11.20 LyS2.reconfigure HomeSyringe @20:12:11.23 LyS1.reconfigure HomeSyringe @20:12:11.30 PinPort.reconfigure HomePP @20:12:11.33 AirS.reconfigure HomeMagSyringe @20:12:11.59 TV.jog -545 @20:12:11.67 PV.jog -1491 @20:12:11.69 LyS2.jog -277 @20:12:11.73 AirS.jog -308 @20:12:11.77 PinPort.jog -109 @20:12:11.82 LyS1.jog -280 @20:12:12.17 ArS.setRawPosition! -50 @20:12:12.22 ArS.seek :home @20:12:12.37 PinPort.setRawPosition! 0 @20:12:12.42 PinPort.seek :home @20:12:12.55 LyS2.setRawPosition! -50 @20:12:12.60 PinPort.reconfigure PPconfig @20:12:12.65 ArS.reconfigure ArSconfig @20:12:12.72 LyS2.seek :home @20:12:12.90 AirS.setRawPosition! -50 @20:12:12.93 LyS1.setRawPosition! -50 @20:12:13.33 AirS.seek :home @20:12:13.40 LyS1.seek :home @20:12:13.54 LyS2.reconfigure LyS2config @20:12:13.57 PV.setRawPosition! -50 @20:12:13.70 PV.seek :home @20:12:13.85 TV.setRawPosition! 0 @20:12:13.89 AirS.reconfigure AirSconfig @20:12:13.92 LyS1.reconfigure LyS1config @20:12:14.07 TV.seek :home @20:12:14.22 TV.reconfigure TVconfig @20:12:14.24 PV.reconfigure PVconfig @20:12:14.61
TV.seek :clear @20:12:16.23 TV.coast @20:12:16.28 SC.reconfigure HomeSC @20:12:16.34 SC.home.jog 0 @20:12:17.11 SC.reconfigure SCconfig @20:12:17.17 SC.seek 56 @20:12:17.26 Selecting Cartridge 56 @20:12:17.53 LyS2.seek :locked @20:12:17.67 PV.seek :locked @20:12:17.84 AirS.seek :locked @20:12:21.93
Powering cartridge bus @20:12:24.06 Gate.setIR 1750,5000,0,6 @20:12:24.18 Gate.setIR 0,5000,0,6 @20:12:24.21 Loaded Archive Cartridge @20:12:24.22 Gate.power :cartridge,:OFF @20:12:24.50 Duration of filtering limited to 1:05:00 @20:12:24.80 Priming sample loop w/100ml, bypass w/10ml @20:12:24.89 SP.setPosition! 0ml @20:12:24.93 Intake.open @20:12:24.96 Exhaust.open @20:12:25.83 Sea @-42.9psia @20:12:25.85 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @20:12:31.59 Vacuum tare is 22% of full scale @20:12:31.61 PB.open @20:12:31.64 Exhaust.close @20:12:32.50 Sea @-42.9psia @20:12:32.59 SP.reconfigure SPprime @20:12:32.65 SP.seek 10ml,1:21 @20:12:43.52 SP.setPosition! 0ml @20:12:43.56 PB.close @20:12:43.61 Exhaust.open @20:12:44.45 Sea @-42.9psia @20:12:44.54 SP.reconfigure SPprime @20:12:44.60 SP.seek 100ml,8:51 @20:13:14.40 Session Begun @20:13:17.78 -> showlog 400 @20:13:44.50 -> Cmd.goalVolume @20:13:52.20 -> Cmd.goalVolume=500 @20:14:18.01 -> SP @20:14:31.97 Pressurizing cartridge to 26psi @20:14:31.99 Intake.close @20:14:32.78 SP.setPosition! 0ml @20:14:32.85 PB.open @20:14:33.67 TV.seek :crack @20:14:34.51 TV.seek :clear @20:14:35.35 Intake.open @20:14:36.80 PB.close @20:14:37.53 Exhaust.close @20:14:38.35 Sea @-42.9psia @20:14:38.48 SP.reconfigure SPcharge @20:14:38.57 SP.seek 7.5ml,1:08 @20:14:43.84 Cartridge pressurized with 0.124ml to 26.3psi @20:14:44.12 Pressure peaked at 26.8psi @20:14:44.14 delay 3 seconds @20:14:47.25 Cartridge at 10.0psi 3 seconds later @20:14:47.27 Intake.close @20:14:48.16 TV.seek :bypass @20:14:49.81 TV.seek :clear @20:14:51.46 Failed Filtering after 0.0ml because Cartridge (or plumbing) leaks @20:14:51.49 Cmd::Error in FILTERING -- No spareArchive cartridges remain @20:14:51.54 Thread::ChildDied in MAIN -- :FILTERING @20:14:51.62 Thread::ChildDied in MAIN_scheduler -- :MAIN @20:14:51.71
SP.coast @20:14:51.83 Session Ended @20:17:56.44PDT24-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @20:17:56.50 >BREAK< ESP3 Gateway v0.99 for cartridge bus @20:17:58.12 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @20:17:59.60 Polling Can every 5:00 @20:17:59.70 Gate.power :analytic1,:ON,limit to 5000mA @20:17:59.71 Gate.power :analytic2,:ON,limit to 5000mA @20:17:59.73 Gate.power :analytic3,:ON,limit to 5000mA @20:18:00.39 ArS.configure ArSconfig @20:18:00.51 LyS1.configure LyS1config @20:18:00.65 LyS2.configure LyS2config @20:18:00.75 AirS.configure AirSconfig @20:18:00.89 TV.configure TVconfig @20:18:01.06 SPR Module 1, FW Rev. B, FPGA Rev. C @20:18:01.08
SP.configure SPconfig @20:18:01.21 SC.reconfigure SCconfig @20:18:01.27 SC.hold @20:18:01.33 SC.configure SCconfig @20:18:01.50 PV.configure PVconfig @20:18:01.63 PinPort.configure PPconfig @20:18:01.77 Sea.configure SPconfig @20:18:01.82 All elf controllers running firmware version 4.46 @20:18:01.90 Intake.close @20:18:01.95 Exhaust.close @20:18:01.99 PB.close @20:18:02.22
Loading /home/esp/esp2/mission/processAll.rb mission @20:18:02.26 Processing all remaining dry cartridges in: {dry: [1, 2, 8, 9, 13, 18, 23, 28, 29, 32, 34, 37, 40, 43, 46, 49, 52, 55], wet: 56} @20:18:02.27 Sampling maximum of 500.0ml per cartridge @20:18:02.29 Fixing samples for 10:00, evacuating once @20:18:02.30 delay 30 seconds @20:18:33.02 SC.seek 56 @20:18:33.09 Selecting Cartridge 55 @20:18:33.53 AirS.seek :clear @20:18:37.77 LyS2.seek :clear @20:18:37.92 PV.seek :clear @20:18:40.66
SC.seek 55 @20:18:41.20 LyS2.seek :locked @20:18:41.36 PV.seek :locked @20:18:41.51 AirS.seek :locked @20:18:45.60
Powering cartridge bus @20:18:47.74 Gate.setIR 1750,5000,0,6 @20:18:47.86 Gate.setIR 0,5000,0,6 @20:18:47.93 Loaded Lyse-n-Go Cartridge @20:18:47.94 PuckHeater.configure HeatConfig @20:18:47.98 Gate.power :heat,:ON,limit to 5000mA @20:18:48.26 Duration of filtering limited to 1:05:00 @20:18:48.55 Priming sample loop w/100ml, bypass w/10ml @20:18:48.63 SP.setPosition! 0ml @20:18:48.67 Intake.open @20:18:48.71 Exhaust.open @20:18:49.57 Sea @-42.9psia @20:18:49.59 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @20:18:55.66 Vacuum tare is 22% of full scale @20:18:55.68 PB.open @20:18:55.71 Exhaust.close @20:18:56.56 Sea @-42.9psia @20:18:56.66 SP.reconfigure SPprime @20:18:56.72 SP.seek 10ml,1:21 @20:19:07.59 SP.setPosition! 0ml @20:19:07.63 PB.close @20:19:07.67 Exhaust.open @20:19:08.52 Sea @-42.9psia @20:19:08.61 SP.reconfigure SPprime @20:19:08.68 SP.seek 100ml,8:51 @20:20:55.52 Pressurizing cartridge to 26psi @20:20:55.54 Intake.close @20:20:56.24 SP.setPosition! 0ml @20:20:56.28 PB.open @20:20:57.06 TV.seek :crack @20:20:57.87 TV.seek :clear @20:20:58.67 Intake.open @20:21:00.08 PB.close @20:21:00.84 Exhaust.close @20:21:01.63 Sea @-42.9psia @20:21:01.72 SP.reconfigure SPcharge @20:21:01.79 SP.seek 7.5ml,1:08 @20:21:12.94 Cartridge pressurized with 0.582ml to 26.4psi @20:21:13.38 delay 3 seconds @20:21:16.48 Cartridge at 20.1psi 3 seconds later @20:21:16.57 TV.seek :cartridge @20:21:18.38 TV.seek :clear @20:21:20.20 Sampling 500.0ml @20:21:20.21 SP.setPosition! 0ml @20:21:20.26 Exhaust.open @20:21:21.07 Sea @-42.9psia @20:21:21.17 SP.reconfigure SPsample @20:21:21.24 SP.seek 500ml,42:12 @20:21:22.22 Cartridge 55 sampling 0/500ml at 0.17ml/s with 1.7psi vacuum -39.0m deep (-42.9psia) @20:22:22.23 Cartridge 55 sampling 35/500ml at 0.58ml/s with 17.5psi vacuum -39.0m deep (-42.9psia) @20:23:22.23 Cartridge 55 sampling 71/500ml at 0.61ml/s with 24.4psi vacuum -39.0m deep (-42.9psia) @20:24:22.22 Cartridge 55 sampling 105/500ml at 0.55ml/s with 15.5psi vacuum -39.0m deep (-42.9psia) @20:25:22.22 Cartridge 55 sampling 140/500ml at 0.60ml/s with 19.0psi vacuum -39.0m deep (-42.9psia) @20:26:22.23 Cartridge 55 sampling 175/500ml at 0.56ml/s with 16.3psi vacuum -39.0m deep (-42.9psia) @20:27:22.22 Cartridge 55 sampling 209/500ml at 0.59ml/s with 19.9psi vacuum -39.0m deep (-42.9psia) @20:28:22.23 Cartridge 55 sampling 243/500ml at 0.56ml/s with 15.9psi vacuum -39.0m deep (-42.9psia) @20:29:22.24 Cartridge 55 sampling 276/500ml at 0.58ml/s with 21.1psi vacuum -39.0m deep (-42.9psia) @20:30:22.22 Cartridge 55 sampling 309/500ml at 0.54ml/s with 19.1psi vacuum -39.0m deep (-42.9psia) @20:31:22.23 Cartridge 55 sampling 341/500ml at 0.55ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @20:32:22.22 Cartridge 55 sampling 375/500ml at 0.57ml/s with 20.8psi vacuum -39.0m deep (-42.9psia) @20:33:22.23 Cartridge 55 sampling 407/500ml at 0.52ml/s with 28.0psi vacuum -39.0m deep (-42.9psia) @20:34:22.23 Cartridge 55 sampling 439/500ml at 0.50ml/s with 16.9psi vacuum -39.0m deep (-42.9psia) @20:35:22.23 Cartridge 55 sampling 470/500ml at 0.53ml/s with 27.3psi vacuum -39.0m deep (-42.9psia) @20:36:22.57 Cartridge 55 sampling 500/500ml at 0.09ml/s with 3.6psi vacuum -39.0m deep (-42.9psia) @20:36:25.29 Intake.close @20:36:25.36 Exhaust.close @20:36:26.15 Sampled 500.0ml @20:36:26.24 TV.seek :bypass @20:36:27.87 TV.seek :clear @20:36:31.32 SPR.command "read_sequences" @20:36:31.35 SPR.command "stats_on" @20:36:31.39 SPR.command "start 2 0" @20:36:33.39 Begun log /var/log/esp/spr18jul2420h.spr @20:36:34.57 Gate.power :heat,:OFF @20:36:34.58 Gate.power :cartridge,:OFF @20:36:39.63 Powering cartridge bus @20:36:41.75 Gate.setIR 1750,5000,0,6 @20:36:41.88 Gate.setIR 0,5000,0,6 @20:36:41.94 Loaded Lyse-n-Go Cartridge @20:36:41.96 PuckHeater.configure HeatConfig @20:36:41.99 Gate.power :heat,:ON,limit to 5000mA @20:36:42.01 Handoff.select :waste @20:36:42.14 ArS.seek empty @20:36:59.86 PinPort.seek :cartridge @20:37:05.58 LyS1.seek 1350ul @20:37:11.52 PV.seek :reagent @20:37:12.39 AirS.seek :tVent @20:37:14.45 delay 2 seconds @20:37:16.48 AirS.reconfigure EvacAirS @20:37:16.53 AirS.seek :evac @20:37:38.85 delay 15 seconds @20:37:53.88 AirS.reconfigure AirSconfig @20:37:53.93 AirS.seek :tVent @20:38:08.16 delay 2 seconds @20:38:10.21 AirS.reconfigure EvacAirS @20:38:10.28 AirS.seek :evac @20:38:32.59 delay 15 seconds @20:38:47.62 AirS.reconfigure AirSconfig @20:38:47.67 AirS.seek :tVent @20:39:01.88 delay 2 seconds @20:39:03.92 AirS.reconfigure EvacAirS @20:39:03.97 AirS.seek :evac @20:39:26.29 delay 15 seconds @20:39:41.32 AirS.reconfigure AirSconfig @20:39:41.39 AirS.seek :tVent @20:39:55.60 delay 2 seconds @20:39:57.63 AirS.reconfigure EvacAirS @20:39:57.69 AirS.seek :evac @20:40:20.00 delay 15 seconds @20:40:35.03 delay 1:30 @20:40:55.57 Session Begun @20:40:59.53 -> AirS.status @20:42:05.05 AirS.reconfigure AirSconfig @20:42:05.14 AirS.seek :fVent @20:42:06.60 delay 15 seconds @20:42:21.64 LyS1.reconfigure SlowLyS1 @20:42:21.80 LyS1.seek 1050ul @20:42:42.91 delay 5 seconds @20:42:47.93 Handoff.select :blocked @20:42:48.14 LyS1.seek 450ul @20:43:30.19 delay 10 seconds @20:43:40.22 AirS.reconfigure SlowAirS @20:43:40.39 AirS.reconfigure to mix into 1.37806 parts LyS1 @20:43:40.90 AirS.seek evac @20:43:40.98 LyS1.seek 300ul @20:43:51.70 AirS.reconfigure AirSconfig @20:43:51.79 AirS.seek :full @20:44:06.04 LyS1.seek :empty @20:44:27.20 PuckHeater.seek 84.3C @20:44:49.19 -> Heat @20:45:09.05 -> Heat @20:45:24.27 -> Heat @20:45:31.68 PuckHeater.hold 15:00,84.3C @21:00:26.31 LyS1.reconfigure LyS1config @21:00:26.41 LyS1.seek 900ul @21:00:34.77 AirS.seek :tVent @21:00:34.89 delay 2 seconds @21:00:36.92 AirS.reconfigure HalfSpeedAirS @21:00:37.02 AirS.seek :evac @21:01:04.81 delay 15 seconds @21:01:19.84 AirS.reconfigure AirSconfig @21:01:20.00 AirS.seek :tVent @21:01:34.25 delay 2 seconds @21:01:36.28 AirS.reconfigure HalfSpeedAirS @21:01:36.34 AirS.seek :evac @21:02:04.15 delay 15 seconds @21:02:19.19 AirS.reconfigure AirSconfig @21:02:19.31 AirS.seek :tVent @21:02:33.56 delay 2 seconds @21:02:35.59 AirS.reconfigure HalfSpeedAirS @21:02:35.68 AirS.seek :evac @21:03:03.44 delay 15 seconds @21:03:18.47 delay 1:00 @21:04:18.49 Handoff.select :waste @21:04:18.64 delay 15 seconds @21:04:33.67 AirS.seek :fVent @21:04:35.90 delay 10 seconds @21:04:45.93 AirS.reconfigure AirSconfig @21:04:46.02 AirS.seek :evac @21:04:47.48 Lysis Recovered in LyS1 @21:04:47.49 Lysis Complete @21:04:47.52 Handoff.select :waste @21:04:47.58 LyS2.seek :full @21:04:47.73 LyS1.reconfigure DAmixLyS1 @21:04:47.81 LyS2.reconfigure DAmixLyS2 @21:04:48.33 LyS2.seek 1480ul @21:04:48.37 LyS1.seek 650ul @21:05:23.48 delay 5 seconds @21:05:28.51 Handoff.select :SPR @21:05:28.64 SPR.command "start 1 14" @21:05:29.79 Running Experiment-Rep #1 @21:05:29.80 delay 10 seconds @21:05:39.84 SPR.command "start 1 0" @21:05:43.66 Waiting up to 4:00 for SPR to enter state 1 @21:07:48.37 SPR.command "hold_on" @21:07:50.21 LyS2.seek 1130ul @21:07:50.29 LyS1.seek 300ul @21:08:39.37 delay 15 seconds @21:08:54.40 SPR.command "hold_off" @21:08:57.04 Waiting up to 25:00 for SPR to enter state 7 @21:29:01.44 Handoff.select :waste @21:29:01.60 LyS1.reconfigure LyS1config @21:29:01.68 LyS2.reconfigure LyS2config @21:29:01.75 Rep #1 SPR Analysis has Completed @21:29:02.38 Ended log /var/log/esp/spr18jul2420h.spr @21:29:02.40 SPR.command "stats_off" @21:29:03.42 Handoff.select :close @21:29:03.95
PV.seek :locked @21:29:04.98 ArS.seek :clear @21:29:05.16 LyS1.seek :clear @21:29:05.36 LyS2.seek :clear @21:29:05.58 AirS.seek :clear @21:29:22.80
ArS.coast @21:29:22.88 LyS1.coast @21:29:22.95 LyS2.coast @21:29:23.02 AirS.coast @21:29:23.10 TV.coast @21:29:23.16 SP.coast @21:29:23.24 SC.coast @21:29:23.33 PV.coast @21:29:23.41 PinPort.coast @21:29:23.63 Gate.power :motor,:OFF @21:29:23.65 Gate.power :heat,:OFF @21:29:23.70 Gate.power :main,:OFF @21:29:23.72 Gate.power :cartridge,:OFF @21:29:23.75 Gate.power :core,:OFF @21:29:23.78 Gate.power :analytic1,:OFF @21:29:23.81 Gate.power :analytic2,:OFF @21:29:23.84 Gate.power :analytic3,:OFF @21:29:23.87 Safely stopped and ready to power off @21:29:24.07 delay 30 seconds @21:29:54.19 Gate.power :main,:ON,limit to 5000mA @21:29:54.22 Gate.power :analytic1,:ON,limit to 5000mA @21:29:54.26 Gate.power :analytic2,:ON,limit to 5000mA @21:29:54.29 Gate.power :analytic3,:ON,limit to 5000mA @21:29:54.40 Powering core bus @21:29:56.80 SPR Module 1, FW Rev. B, FPGA Rev. C @21:29:56.94
ArS.configure ArSconfig @21:29:57.08 LyS1.configure LyS1config @21:29:57.25 LyS2.configure LyS2config @21:29:57.38 AirS.configure AirSconfig @21:29:57.54 TV.configure TVconfig @21:29:57.70 SP.configure SPconfig @21:29:57.86 SC.configure SCconfig @21:29:58.02 PV.configure PVconfig @21:29:58.19 PinPort.configure PPconfig @21:29:58.35 Sea.configure SPconfig @21:29:58.44 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @21:29:58.46 Gate.power :motor,:ON,limit to 5000mA @21:29:59.19 LyS2.reconfigure LyS2config @21:29:59.28 ArS.reconfigure ArSconfig @21:29:59.33 PinPort.reconfigure PPconfig @21:29:59.37 TV.reconfigure TVconfig @21:29:59.45 AirS.reconfigure AirSconfig @21:29:59.50 PV.reconfigure PVconfig @21:29:59.59 LyS1.reconfigure LyS1config @21:29:59.89 LyS2.home.jog 0 @21:29:59.93 ArS.home.jog 0 @21:29:59.98 PinPort.home.jog 0 @21:30:00.01 TV.home.jog 0 @21:30:00.07 AirS.home.jog 0 @21:30:00.12 PV.home.jog 0 @21:30:00.17 LyS1.home.jog 0 @21:30:01.20 PV.reconfigure HomePV @21:30:01.34 AirS.reconfigure HomeMagSyringe @21:30:01.41 LyS1.reconfigure HomeSyringe @21:30:01.45 PinPort.reconfigure HomePP @21:30:01.51 TV.reconfigure HomeTV @21:30:01.95 LyS2.reconfigure HomeSyringe @21:30:02.00 ArS.reconfigure HomeMagSyringe @21:30:02.30 PV.jog -1499 @21:30:02.36 AirS.jog -307 @21:30:02.43 LyS1.jog -276 @21:30:02.51 PinPort.jog -2410 @21:30:02.55 TV.jog -550 @21:30:02.60 ArS.jog -276 @21:30:02.70 LyS2.jog -266 @21:30:03.38 AirS.setRawPosition! -50 @21:30:03.47 AirS.seek :home @21:30:03.62 LyS2.setRawPosition! -50 @21:30:03.65 LyS1.setRawPosition! -50 @21:30:03.68 ArS.setRawPosition! -50 @21:30:03.85 LyS2.seek :home @21:30:03.89 LyS1.seek :home @21:30:04.01 ArS.seek :home @21:30:04.28 PV.setRawPosition! -50 @21:30:04.32 AirS.reconfigure AirSconfig @21:30:04.40 PV.seek :home @21:30:04.64 LyS2.reconfigure LyS2config @21:30:04.68 LyS1.reconfigure LyS1config @21:30:04.73 ArS.reconfigure ArSconfig @21:30:04.98 TV.setRawPosition! 0 @21:30:05.01 PV.reconfigure PVconfig @21:30:05.14 TV.seek :home @21:30:05.30 TV.reconfigure TVconfig @21:30:08.60 PinPort.setRawPosition! 0 @21:30:08.68 PinPort.seek :home @21:30:08.79 PinPort.reconfigure PPconfig @21:30:09.36
TV.seek :clear @21:30:10.99 TV.coast @21:30:11.07 SC.reconfigure HomeSC @21:30:11.16 SC.home.jog 0 @21:30:11.95 SC.reconfigure SCconfig @21:30:12.03 SC.seek 55 @21:30:12.18 Selecting Cartridge 52 @21:30:12.57 SC.seek 52 @21:30:13.55 LyS2.seek :locked @21:30:13.75 PV.seek :locked @21:30:13.93 AirS.seek :locked @21:30:18.03
Powering cartridge bus @21:30:20.17 Gate.setIR 1750,5000,0,6 @21:30:20.29 Gate.setIR 0,5000,0,6 @21:30:20.35 Loaded Archive Cartridge @21:30:20.36 Gate.power :cartridge,:OFF @21:30:20.68 Duration of filtering limited to 1:05:00 @21:30:21.01 Priming sample loop w/100ml, bypass w/10ml @21:30:21.13 SP.setPosition! 0ml @21:30:21.20 Intake.open @21:30:21.24 Exhaust.open @21:30:22.47 Sea @-42.9psia @21:30:22.49 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @21:30:28.22 Vacuum tare is 20% of full scale @21:30:28.24 PB.open @21:30:28.33 Exhaust.close @21:30:29.19 Sea @-42.9psia @21:30:29.31 SP.reconfigure SPprime @21:30:29.40 SP.seek 10ml,1:21 @21:30:40.31 SP.setPosition! 0ml @21:30:40.39 PB.close @21:30:40.43 Exhaust.open @21:30:41.31 Sea @-42.9psia @21:30:41.43 SP.reconfigure SPprime @21:30:41.53 SP.seek 100ml,8:51 @21:32:28.33 Pressurizing cartridge to 26psi @21:32:28.34 Intake.close @21:32:29.10 SP.setPosition! 0ml @21:32:29.17 PB.open @21:32:29.99 TV.seek :crack @21:32:30.82 TV.seek :clear @21:32:31.65 Intake.open @21:32:33.13 PB.close @21:32:33.93 Exhaust.close @21:32:34.72 Sea @-42.9psia @21:32:34.84 SP.reconfigure SPcharge @21:32:34.93 SP.seek 7.5ml,1:08 @21:32:49.29 Cartridge pressurized with 0.960ml to 26.0psi @21:32:49.58 delay 3 seconds @21:32:52.67 Cartridge at 19.3psi 3 seconds later @21:32:52.79 TV.seek :cartridge @21:32:54.64 TV.seek :clear @21:32:56.48 Sampling 500.0ml @21:32:56.50 SP.setPosition! 0ml @21:32:56.57 Exhaust.open @21:32:57.41 Sea @-42.9psia @21:32:57.54 SP.reconfigure SPsample @21:32:57.63 SP.seek 500ml,42:12 @21:32:58.58 Cartridge 52 sampling 0/500ml at 0.16ml/s with 2.9psi vacuum -39.0m deep (-42.9psia) @21:33:58.58 Cartridge 52 sampling 32/500ml at 0.53ml/s with 18.3psi vacuum -39.0m deep (-42.9psia) @21:34:58.59 Cartridge 52 sampling 65/500ml at 0.54ml/s with 22.4psi vacuum -39.0m deep (-42.9psia) @21:35:58.58 Cartridge 52 sampling 96/500ml at 0.52ml/s with 19.7psi vacuum -39.0m deep (-42.9psia) @21:36:58.59 Cartridge 52 sampling 128/500ml at 0.49ml/s with 21.9psi vacuum -39.0m deep (-42.9psia) @21:37:58.59 Cartridge 52 sampling 158/500ml at 0.50ml/s with 27.7psi vacuum -39.0m deep (-42.9psia) @21:38:58.59 Cartridge 52 sampling 188/500ml at 0.51ml/s with 20.0psi vacuum -39.0m deep (-42.9psia) @21:39:58.59 Cartridge 52 sampling 217/500ml at 0.52ml/s with 23.2psi vacuum -39.0m deep (-42.9psia) @21:40:58.59 Cartridge 52 sampling 245/500ml at 0.48ml/s with 26.9psi vacuum -39.0m deep (-42.9psia) @21:41:58.58 Cartridge 52 sampling 273/500ml at 0.44ml/s with 24.8psi vacuum -39.0m deep (-42.9psia) @21:42:58.93 Cartridge 52 sampling 301/500ml at 0.47ml/s with 15.7psi vacuum -39.0m deep (-42.9psia) @21:43:58.59 Cartridge 52 sampling 328/500ml at 0.42ml/s with 19.1psi vacuum -39.0m deep (-42.9psia) @21:44:32.35 Session Begun @21:44:36.08 -> showlog 30 @21:44:58.58 Cartridge 52 sampling 354/500ml at 0.41ml/s with 17.3psi vacuum -39.0m deep (-42.9psia) @21:45:58.59 Cartridge 52 sampling 379/500ml at 0.39ml/s with 24.2psi vacuum -39.0m deep (-42.9psia) @21:46:58.59 Cartridge 52 sampling 404/500ml at 0.43ml/s with 26.3psi vacuum -39.0m deep (-42.9psia) @21:47:58.58 Cartridge 52 sampling 428/500ml at 0.42ml/s with 26.1psi vacuum -39.0m deep (-42.9psia) @21:48:58.59 Cartridge 52 sampling 452/500ml at 0.38ml/s with 18.1psi vacuum -39.0m deep (-42.9psia) @21:49:58.59 Cartridge 52 sampling 475/500ml at 0.36ml/s with 25.0psi vacuum -39.0m deep (-42.9psia) @21:50:58.59 Cartridge 52 sampling 498/500ml at 0.37ml/s with 20.9psi vacuum -39.0m deep (-42.9psia) @21:51:03.55 Intake.close @21:51:03.59 Exhaust.close @21:51:04.46 Sampled 500.0ml @21:51:04.59 TV.seek :bypass @21:51:06.23 TV.seek :clear @21:51:08.20 PV.seek :reagent @21:51:09.10 Sample loop closed. Ready to Deliver Reagents @21:51:09.13 ArS.seek :sealed @21:51:13.50 ArS.seek :evac @21:51:27.50 delay 20 seconds @21:51:47.54 ArS.seek :clear @21:52:05.29 RNAlater soak for 10:00 @21:52:23.42 -> Cmd.slots spareGeneric:2, spareLysing:2, spareArchive:2 @21:52:23.47 NoMethodError in brent#2 -- undefined method `+' for :spareArchive:Symbol @21:52:52.25 -> Cmd.slots @21:52:52.28 NoMethodError in brent#2 -- undefined method `+' for :spareArchive:Symbol @21:53:03.79 -> Cmd.map @21:53:46.81 -> Cmd.slots @21:53:46.88 NoMethodError in brent#2 -- undefined method `+' for :spareArchive:Symbol @21:53:49.21 -> backtrace @22:02:05.33 ArS.seek :tVent @22:02:07.96 delay 1 second @22:02:08.99 ArS.seek :evac @22:02:24.66 delay 10 seconds @22:02:34.70 Sample preserved and evacuated @22:02:34.90
PV.seek :locked @22:02:35.93 ArS.seek :clear @22:02:36.20 LyS2.seek :clear @22:02:36.41 AirS.seek :clear @22:02:53.75
ArS.coast @22:02:53.84 LyS1.coast @22:02:53.92 LyS2.coast @22:02:54.01 AirS.coast @22:02:54.09 TV.coast @22:02:54.18 SP.coast @22:02:54.25 SC.coast @22:02:54.36 PV.coast @22:02:54.45 PinPort.coast @22:02:54.68 Gate.power :motor,:OFF @22:02:54.70 Gate.power :main,:OFF @22:02:54.77 Gate.power :core,:OFF @22:02:54.80 Gate.power :analytic1,:OFF @22:02:54.85 Gate.power :analytic2,:OFF @22:02:54.89 Gate.power :analytic3,:OFF @22:02:54.95 Safely stopped and ready to power off @22:02:55.15 delay 30 seconds @22:03:25.28 Gate.power :main,:ON,limit to 5000mA @22:03:25.30 Gate.power :analytic1,:ON,limit to 5000mA @22:03:25.37 Gate.power :analytic2,:ON,limit to 5000mA @22:03:25.43 Gate.power :analytic3,:ON,limit to 5000mA @22:03:25.54 Powering core bus @22:03:27.91 SPR Module 1, FW Rev. B, FPGA Rev. C @22:03:28.11
ArS.configure ArSconfig @22:03:28.63 LyS1.configure LyS1config @22:03:28.80 LyS2.configure LyS2config @22:03:28.95 AirS.configure AirSconfig @22:03:29.12 TV.configure TVconfig @22:03:29.29 SP.configure SPconfig @22:03:29.47 SC.configure SCconfig @22:03:29.64 PV.configure PVconfig @22:03:29.81 PinPort.configure PPconfig @22:03:29.98 Sea.configure SPconfig @22:03:30.08 All elf controllers running firmware version 4.46 @22:03:30.11 Gate.power :motor,:ON,limit to 5000mA @22:03:30.38 ArS.reconfigure ArSconfig @22:03:30.80 ArS.home.jog 0 @22:03:31.06 PV.reconfigure PVconfig @22:03:31.12 TV.reconfigure TVconfig @22:03:31.20 PinPort.reconfigure PPconfig @22:03:31.27 LyS2.reconfigure LyS2config @22:03:31.33 LyS1.reconfigure LyS1config @22:03:31.47 AirS.reconfigure AirSconfig @22:03:31.72 PV.home.jog 0 @22:03:31.75 TV.home.jog 0 @22:03:31.95 PinPort.home.jog 0 @22:03:32.11 LyS2.home.jog 0 @22:03:32.15 LyS1.home.jog 0 @22:03:32.20 AirS.home.jog 0 @22:03:32.58 ArS.reconfigure HomeMagSyringe @22:03:33.08 ArS.jog -277 @22:03:33.16 PV.reconfigure HomePV @22:03:33.22 AirS.reconfigure HomeMagSyringe @22:03:33.27 LyS2.reconfigure HomeSyringe @22:03:33.35 TV.reconfigure HomeTV @22:03:33.44 LyS1.reconfigure HomeSyringe @22:03:33.52 PinPort.reconfigure HomePP @22:03:33.87 PV.jog -1501 @22:03:33.91 AirS.jog -308 @22:03:33.95 LyS2.jog -266 @22:03:34.04 TV.jog -549 @22:03:34.09 LyS1.jog -274 @22:03:34.15 PinPort.jog -107 @22:03:34.57 ArS.setRawPosition! -50 @22:03:34.66 ArS.seek :home @22:03:34.79 PinPort.setRawPosition! 0 @22:03:34.89 PinPort.seek :home @22:03:35.09 AirS.setRawPosition! -50 @22:03:35.14 LyS2.setRawPosition! -50 @22:03:35.19 LyS1.setRawPosition! -50 @22:03:35.25 PinPort.reconfigure PPconfig @22:03:35.33 ArS.reconfigure ArSconfig @22:03:35.57 AirS.seek :home @22:03:35.63 LyS2.seek :home @22:03:35.76 LyS1.seek :home @22:03:36.06 PV.setRawPosition! -50 @22:03:36.16 PV.seek :home @22:03:36.70 AirS.reconfigure AirSconfig @22:03:36.73 LyS2.reconfigure LyS2config @22:03:36.78 TV.setRawPosition! 0 @22:03:36.88 LyS1.reconfigure LyS1config @22:03:37.16 TV.seek :home @22:03:37.33 TV.reconfigure TVconfig @22:03:37.36 PV.reconfigure PVconfig @22:03:37.66
TV.seek :clear @22:03:39.32 TV.coast @22:03:39.42 SC.reconfigure HomeSC @22:03:39.51 SC.home.jog 0 @22:03:40.33 SC.reconfigure SCconfig @22:03:40.43 SC.seek 52 @22:03:40.56 Selecting Cartridge 49 @22:03:40.97 SC.seek 49 @22:03:41.97 LyS2.seek :locked @22:03:42.17 PV.seek :locked @22:03:42.38 AirS.seek :locked @22:03:46.50
Powering cartridge bus @22:03:48.63 Gate.setIR 1750,5000,0,6 @22:03:48.76 Gate.setIR 0,5000,0,6 @22:03:48.86 Loaded Lyse-n-Go Cartridge @22:03:48.88 PuckHeater.configure HeatConfig @22:03:48.98 Gate.power :heat,:ON,limit to 5000mA @22:03:49.30 Duration of filtering limited to 1:05:00 @22:03:49.65 Priming sample loop w/100ml, bypass w/10ml @22:03:49.78 SP.setPosition! 0ml @22:03:49.86 Intake.open @22:03:49.90 Exhaust.open @22:03:50.82 Sea @-42.9psia @22:03:50.85 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @22:03:56.62 Session Begun @22:03:56.89 Vacuum tare is 20% of full scale @22:03:56.93 PB.open @22:03:57.07 Exhaust.close @22:03:58.02 Sea @-42.9psia @22:03:58.17 SP.reconfigure SPprime @22:03:58.30 SP.seek 10ml,1:21 @22:04:09.23 SP.setPosition! 0ml @22:04:09.34 PB.close @22:04:09.38 Exhaust.open @22:04:10.33 Sea @-42.9psia @22:04:10.81 SP.reconfigure SPprime @22:04:10.95 SP.seek 100ml,8:51 @22:05:57.53 Pressurizing cartridge to 26psi @22:05:57.56 Intake.close @22:05:58.44 SP.setPosition! 0ml @22:05:58.54 PB.open @22:05:59.40 TV.seek :crack @22:06:00.27 TV.seek :clear @22:06:01.13 Intake.open @22:06:02.61 PB.close @22:06:03.38 Exhaust.close @22:06:04.23 Sea @-42.9psia @22:06:04.37 SP.reconfigure SPcharge @22:06:04.49 SP.seek 7.5ml,1:08 @22:06:10.92 Cartridge pressurized with 0.187ml to 26.1psi @22:06:11.24 delay 3 seconds @22:06:14.34 Cartridge at 15.9psi 3 seconds later @22:06:14.48 TV.seek :cartridge @22:06:16.35 TV.seek :clear @22:06:18.22 Sampling 500.0ml @22:06:18.24 SP.setPosition! 0ml @22:06:18.38 Exhaust.open @22:06:19.17 Sea @-42.9psia @22:06:19.35 SP.reconfigure SPsample @22:06:19.47 SP.seek 500ml,42:12 @22:06:20.34 Cartridge 49 sampling 0/500ml at 0.14ml/s with 3.0psi vacuum -39.0m deep (-42.9psia) @22:07:20.34 Cartridge 49 sampling 35/500ml at 0.56ml/s with 20.6psi vacuum -39.0m deep (-42.9psia) @22:08:20.34 Cartridge 49 sampling 70/500ml at 0.56ml/s with 17.7psi vacuum -39.0m deep (-42.9psia) @22:09:20.34 Cartridge 49 sampling 104/500ml at 0.57ml/s with 18.5psi vacuum -39.0m deep (-42.9psia) @22:10:20.34 Cartridge 49 sampling 138/500ml at 0.54ml/s with 23.2psi vacuum -39.0m deep (-42.9psia) @22:11:20.35 Cartridge 49 sampling 172/500ml at 0.57ml/s with 21.8psi vacuum -39.0m deep (-42.9psia) @22:12:20.34 Cartridge 49 sampling 206/500ml at 0.59ml/s with 24.0psi vacuum -39.0m deep (-42.9psia) @22:13:20.35 Cartridge 49 sampling 240/500ml at 0.55ml/s with 19.6psi vacuum -39.0m deep (-42.9psia) @22:14:20.34 Cartridge 49 sampling 273/500ml at 0.57ml/s with 24.9psi vacuum -39.0m deep (-42.9psia) @22:15:20.35 Cartridge 49 sampling 306/500ml at 0.57ml/s with 18.0psi vacuum -39.0m deep (-42.9psia) @22:16:20.34 Cartridge 49 sampling 340/500ml at 0.54ml/s with 19.3psi vacuum -39.0m deep (-42.9psia) @22:17:20.34 Cartridge 49 sampling 372/500ml at 0.56ml/s with 16.9psi vacuum -39.0m deep (-42.9psia) @22:18:20.34 Cartridge 49 sampling 404/500ml at 0.50ml/s with 22.5psi vacuum -39.0m deep (-42.9psia) @22:19:20.34 Cartridge 49 sampling 436/500ml at 0.53ml/s with 23.7psi vacuum -39.0m deep (-42.9psia) @22:20:20.34 Cartridge 49 sampling 467/500ml at 0.54ml/s with 23.1psi vacuum -39.0m deep (-42.9psia) @22:21:20.34 Cartridge 49 sampling 497/500ml at 0.52ml/s with 13.1psi vacuum -39.0m deep (-42.9psia) @22:21:30.00 Intake.close @22:21:30.14 Exhaust.close @22:21:30.88 Sampled 500.0ml @22:21:31.03 TV.seek :bypass @22:21:32.68 TV.seek :clear @22:21:36.55 SPR.command "read_sequences" @22:21:36.64 SPR.command "stats_on" @22:21:37.40 SPR.command "start 2 0" @22:21:39.40 Begun log /var/log/esp/spr18jul2422h.spr AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @22:21:42.51 Gate.power :heat,:OFF @22:21:42.54 Gate.power :cartridge,:OFF @22:21:47.64 Powering cartridge bus @22:21:49.77 Gate.setIR 1750,5000,0,6 @22:21:49.90 Gate.setIR 0,5000,0,6 @22:21:50.01 Loaded Lyse-n-Go Cartridge @22:21:50.03 PuckHeater.configure HeatConfig @22:21:50.15 Gate.power :heat,:ON,limit to 5000mA @22:21:50.19 Handoff.select :waste @22:21:50.41 ArS.seek empty @22:22:08.20 PinPort.seek :cartridge @22:22:13.99 LyS1.seek 1350ul @22:22:19.99 PV.seek :reagent @22:22:20.94 AirS.seek :tVent @22:22:23.06 delay 2 seconds @22:22:25.09 AirS.reconfigure EvacAirS @22:22:25.23 AirS.seek :evac @22:22:47.57 delay 15 seconds @22:23:02.60 AirS.reconfigure AirSconfig @22:23:02.72 AirS.seek :tVent @22:23:16.97 delay 2 seconds @22:23:19.00 AirS.reconfigure EvacAirS @22:23:19.13 AirS.seek :evac @22:23:41.48 delay 15 seconds @22:23:56.51 AirS.reconfigure AirSconfig @22:23:56.63 AirS.seek :tVent @22:24:10.89 delay 2 seconds @22:24:12.92 AirS.reconfigure EvacAirS @22:24:13.09 AirS.seek :evac @22:24:35.42 delay 15 seconds @22:24:50.45 AirS.reconfigure AirSconfig @22:24:50.57 AirS.seek :tVent @22:25:04.83 delay 2 seconds @22:25:06.86 AirS.reconfigure EvacAirS @22:25:06.99 AirS.seek :evac @22:25:29.34 delay 15 seconds @22:25:44.37 delay 1:30 @22:27:14.42 AirS.reconfigure AirSconfig @22:27:14.53 AirS.seek :fVent @22:27:16.05 delay 15 seconds @22:27:31.08 LyS1.reconfigure SlowLyS1 @22:27:31.27 LyS1.seek 1050ul @22:27:52.39 delay 5 seconds @22:27:57.41 Handoff.select :blocked @22:27:57.65 LyS1.seek 450ul @22:28:39.72 delay 10 seconds @22:28:49.77 AirS.reconfigure SlowAirS @22:28:49.95 AirS.reconfigure to mix into 1.37806 parts LyS1 @22:28:50.50 AirS.seek evac @22:28:50.56 LyS1.seek 300ul @22:29:01.31 AirS.reconfigure AirSconfig @22:29:01.43 AirS.seek :full @22:29:15.69 LyS1.seek :empty @22:29:36.86 PuckHeater.seek 84.3C @22:30:15.57 PuckHeater.hold 15:00,84.3C @22:45:16.46 LyS1.reconfigure LyS1config @22:45:16.58 LyS1.seek 900ul @22:45:25.00 AirS.seek :tVent @22:45:25.18 delay 2 seconds @22:45:27.21 AirS.reconfigure HalfSpeedAirS @22:45:27.34 AirS.seek :evac @22:45:55.13 delay 15 seconds @22:46:10.16 AirS.reconfigure AirSconfig @22:46:10.28 AirS.seek :tVent @22:46:24.55 delay 2 seconds @22:46:26.58 AirS.reconfigure HalfSpeedAirS @22:46:26.72 AirS.seek :evac @22:46:54.51 delay 15 seconds @22:47:09.54 AirS.reconfigure AirSconfig @22:47:09.66 AirS.seek :tVent @22:47:23.99 delay 2 seconds @22:47:26.02 AirS.reconfigure HalfSpeedAirS @22:47:26.14 AirS.seek :evac @22:47:53.97 delay 15 seconds @22:48:09.00 delay 1:00 @22:49:09.01 Handoff.select :waste @22:49:09.19 delay 15 seconds @22:49:24.22 AirS.seek :fVent @22:49:26.48 delay 10 seconds @22:49:36.51 AirS.reconfigure AirSconfig @22:49:36.62 AirS.seek :evac @22:49:38.13 Lysis Recovered in LyS1 @22:49:38.14 Lysis Complete @22:49:38.16 Handoff.select :waste @22:49:38.29 LyS2.seek :full @22:49:38.44 LyS1.reconfigure DAmixLyS1 @22:49:38.54 LyS2.reconfigure DAmixLyS2 @22:49:39.08 LyS2.seek 1480ul @22:49:39.14 LyS1.seek 650ul @22:50:14.32 delay 5 seconds @22:50:19.35 Handoff.select :SPR @22:50:19.52 SPR.command "start 1 14" @22:50:20.70 Running Experiment-Rep #1 @22:50:20.72 delay 10 seconds @22:50:30.76 SPR.command "start 1 0" @22:50:34.06 Waiting up to 4:00 for SPR to enter state 1 @22:52:38.35 SPR.command "hold_on" @22:52:40.18 LyS2.seek 1130ul @22:52:40.25 LyS1.seek 300ul @22:53:29.36 delay 15 seconds @22:53:44.40 SPR.command "hold_off" @22:53:47.01 Waiting up to 25:00 for SPR to enter state 7 @23:13:51.49 Handoff.select :waste @23:13:51.67 LyS1.reconfigure LyS1config @23:13:51.78 LyS2.reconfigure LyS2config @23:13:51.88 Rep #1 SPR Analysis has Completed @23:13:52.35 Ended log /var/log/esp/spr18jul2422h.spr @23:13:52.37 SPR.command "stats_off" @23:13:52.88 Handoff.select :close @23:13:53.14
PV.seek :locked @23:13:54.19 ArS.seek :clear @23:13:54.40 LyS1.seek :clear @23:13:54.61 LyS2.seek :clear @23:13:54.83 AirS.seek :clear @23:14:12.03
ArS.coast @23:14:12.14 LyS1.coast @23:14:12.24 LyS2.coast @23:14:12.35 AirS.coast @23:14:12.44 TV.coast @23:14:12.54 SP.coast @23:14:12.63 SC.coast @23:14:12.73 PV.coast @23:14:12.83 PinPort.coast @23:14:13.08 Gate.power :motor,:OFF @23:14:13.11 Gate.power :heat,:OFF @23:14:13.18 Gate.power :main,:OFF @23:14:13.24 Gate.power :cartridge,:OFF @23:14:13.29 Gate.power :core,:OFF @23:14:13.38 Gate.power :analytic1,:OFF @23:14:13.41 Gate.power :analytic2,:OFF @23:14:13.49 Gate.power :analytic3,:OFF @23:14:13.51 Safely stopped and ready to power off @23:14:13.74 delay 30 seconds @23:14:43.86 Gate.power :main,:ON,limit to 5000mA @23:14:43.88 Gate.power :analytic1,:ON,limit to 5000mA @23:14:44.00 Gate.power :analytic2,:ON,limit to 5000mA @23:14:44.04 Gate.power :analytic3,:ON,limit to 5000mA @23:14:44.17 Powering core bus @23:14:46.52 SPR Module 1, FW Rev. B, FPGA Rev. C @23:14:46.76
ArS.configure ArSconfig @23:14:46.92 LyS1.configure LyS1config @23:14:47.10 LyS2.configure LyS2config @23:14:47.27 AirS.configure AirSconfig @23:14:47.45 TV.configure TVconfig @23:14:47.65 SP.configure SPconfig @23:14:47.84 SC.configure SCconfig @23:14:48.03 PV.configure PVconfig @23:14:48.22 PinPort.configure PPconfig @23:14:48.41 Sea.configure SPconfig @23:14:48.52 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @23:14:48.57 Gate.power :motor,:ON,limit to 5000mA @23:14:48.80 ArS.reconfigure ArSconfig @23:14:49.28 ArS.home.jog 0 @23:14:49.49 PinPort.reconfigure PPconfig @23:14:49.60 LyS2.reconfigure LyS2config @23:14:49.69 TV.reconfigure TVconfig @23:14:49.76 AirS.reconfigure AirSconfig @23:14:49.80 LyS1.reconfigure LyS1config @23:14:49.89 PV.reconfigure PVconfig @23:14:50.29 PinPort.home.jog 0 @23:14:50.33 LyS2.home.jog 0 @23:14:50.44 TV.home.jog 0 @23:14:50.52 LyS1.home.jog 0 @23:14:50.65 AirS.home.jog 0 @23:14:50.69 PV.home.jog 0 @23:14:51.72 ArS.reconfigure HomeMagSyringe @23:14:51.99 ArS.jog -278 @23:14:52.20 TV.reconfigure HomeTV @23:14:52.32 PV.reconfigure HomePV @23:14:52.39 LyS2.reconfigure HomeSyringe @23:14:52.45 PinPort.reconfigure HomePP @23:14:52.54 LyS1.reconfigure HomeSyringe @23:14:52.72 AirS.reconfigure HomeMagSyringe @23:14:52.94 TV.jog -551 @23:14:52.97 LyS2.jog -265 @23:14:53.13 PinPort.jog -2410 @23:14:53.16 PV.jog -1509 @23:14:53.21 LyS1.jog -274 @23:14:53.44 AirS.jog -308 @23:14:53.70 ArS.setRawPosition! -50 @23:14:53.81 ArS.seek :home @23:14:53.96 LyS2.setRawPosition! -50 @23:14:54.03 LyS2.seek :home @23:14:54.25 AirS.setRawPosition! -50 @23:14:54.28 LyS1.setRawPosition! -50 @23:14:54.35 ArS.reconfigure ArSconfig @23:14:54.57 AirS.seek :home @23:14:54.62 LyS1.seek :home @23:14:54.92 LyS2.reconfigure LyS2config @23:14:54.95 PV.setRawPosition! -50 @23:14:55.18 PV.seek :home @23:14:55.34 TV.setRawPosition! 0 @23:14:55.38 AirS.reconfigure AirSconfig @23:14:55.43 LyS1.reconfigure LyS1config @23:14:55.69 TV.seek :home @23:14:55.90 PV.reconfigure PVconfig @23:14:55.93 TV.reconfigure TVconfig @23:14:59.25 PinPort.setRawPosition! 0 @23:14:59.36 PinPort.seek :home @23:14:59.49 PinPort.reconfigure PPconfig @23:15:00.08
TV.seek :clear @23:15:01.74 TV.coast @23:15:01.85 SC.reconfigure HomeSC @23:15:01.95 SC.home.jog 0 @23:15:02.75 SC.reconfigure SCconfig @23:15:02.86 SC.seek 49 @23:15:02.98 Selecting Cartridge 46 @23:15:03.40 SC.seek 46 @23:15:04.42 LyS2.seek :locked @23:15:04.63 PV.seek :locked @23:15:04.83 AirS.seek :locked @23:15:09.32
Powering cartridge bus @23:15:11.45 Gate.setIR 1750,5000,0,6 @23:15:11.58 Gate.setIR 0,5000,0,6 @23:15:11.69 Loaded Lyse-n-Go Cartridge @23:15:11.71 PuckHeater.configure HeatConfig @23:15:11.84 Gate.power :heat,:ON,limit to 5000mA @23:15:12.18 Duration of filtering limited to 1:05:00 @23:15:12.53 Priming sample loop w/100ml, bypass w/10ml @23:15:12.67 SP.setPosition! 0ml @23:15:12.74 Intake.open @23:15:12.79 Exhaust.open @23:15:13.71 Sea @-42.9psia @23:15:13.73 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @23:15:19.50 Vacuum tare is 20% of full scale @23:15:19.52 PB.open @23:15:19.60 Exhaust.close @23:15:20.53 Sea @-42.9psia @23:15:20.68 SP.reconfigure SPprime @23:15:20.79 SP.seek 10ml,1:21 @23:15:31.72 SP.setPosition! 0ml @23:15:31.82 PB.close @23:15:31.86 Exhaust.open @23:15:32.82 Sea @-42.9psia @23:15:32.97 SP.reconfigure SPprime @23:15:33.08 SP.seek 100ml,8:51 @23:17:20.35 Pressurizing cartridge to 26psi @23:17:20.36 Intake.close @23:17:21.20 SP.setPosition! 0ml @23:17:21.30 PB.open @23:17:22.15 TV.seek :crack @23:17:23.00 TV.seek :clear @23:17:23.85 Intake.open @23:17:25.31 PB.close @23:17:26.06 Exhaust.close @23:17:26.93 Sea @-42.9psia @23:17:27.10 SP.reconfigure SPcharge @23:17:27.25 SP.seek 7.5ml,1:08 @23:17:41.76 Cartridge pressurized with 0.977ml to 26.2psi @23:17:42.09 delay 3 seconds @23:17:45.18 Cartridge at 21.2psi 3 seconds later @23:17:45.33 TV.seek :cartridge @23:17:47.16 TV.seek :clear @23:17:49.04 Sampling 500.0ml @23:17:49.06 SP.setPosition! 0ml @23:17:49.19 Exhaust.open @23:17:50.07 Sea @-42.9psia @23:17:50.23 SP.reconfigure SPsample @23:17:50.35 SP.seek 500ml,42:12 @23:17:51.24 Cartridge 46 sampling 0/500ml at 0.15ml/s with 2.7psi vacuum -39.0m deep (-42.9psia) @23:18:51.58 Cartridge 46 sampling 35/500ml at 0.59ml/s with 25.5psi vacuum -39.0m deep (-42.9psia) @23:19:51.22 Cartridge 46 sampling 69/500ml at 0.59ml/s with 24.0psi vacuum -39.0m deep (-42.9psia) @23:20:51.24 Cartridge 46 sampling 103/500ml at 0.57ml/s with 24.1psi vacuum -39.0m deep (-42.9psia) @23:21:51.22 Cartridge 46 sampling 137/500ml at 0.57ml/s with 24.6psi vacuum -39.0m deep (-42.9psia) @23:22:51.24 Cartridge 46 sampling 171/500ml at 0.53ml/s with 17.7psi vacuum -39.0m deep (-42.9psia) @23:23:04.10 -> reload Cmd @23:23:07.60 -> Cmd.slots @23:23:07.67 NoMethodError in brent#2 -- undefined method `+' for :spareArchive:Symbol @23:23:20.37 -> Cmd.maps @23:23:20.40 NoMethodError in brent#2 -- undefined method `maps' for Cmd:Module @23:23:22.72 -> Cmd.map @23:23:34.66 -> list Cmd.method :slots @23:23:51.22 Cartridge 46 sampling 204/500ml at 0.57ml/s with 19.2psi vacuum -39.0m deep (-42.9psia) @23:24:51.25 Cartridge 46 sampling 236/500ml at 0.54ml/s with 26.9psi vacuum -39.0m deep (-42.9psia) @23:24:59.11 -> reload Cmd @23:25:02.57 -> Cmd.slots @23:25:51.22 Cartridge 46 sampling 269/500ml at 0.55ml/s with 15.3psi vacuum -39.0m deep (-42.9psia) @23:26:51.23 Cartridge 46 sampling 301/500ml at 0.57ml/s with 23.6psi vacuum -39.0m deep (-42.9psia) @23:27:51.23 Cartridge 46 sampling 333/500ml at 0.54ml/s with 15.5psi vacuum -39.0m deep (-42.9psia) @23:28:51.23 Cartridge 46 sampling 365/500ml at 0.51ml/s with 19.3psi vacuum -39.0m deep (-42.9psia) @23:29:51.22 Cartridge 46 sampling 396/500ml at 0.50ml/s with 21.1psi vacuum -39.0m deep (-42.9psia) @23:30:51.24 Cartridge 46 sampling 426/500ml at 0.50ml/s with 21.5psi vacuum -39.0m deep (-42.9psia) @23:31:51.22 Cartridge 46 sampling 456/500ml at 0.49ml/s with 21.4psi vacuum -39.0m deep (-42.9psia) @23:32:51.22 Cartridge 46 sampling 485/500ml at 0.51ml/s with 23.2psi vacuum -39.0m deep (-42.9psia) @23:33:26.79 Intake.close @23:33:26.90 Exhaust.close @23:33:27.73 Sampled 500.0ml @23:33:27.88 TV.seek :bypass @23:33:29.90 TV.seek :clear @23:33:33.42 SPR.command "read_sequences" @23:33:33.84 SPR.command "stats_on" @23:33:33.93 SPR.command "start 2 0" @23:33:35.93 Begun log /var/log/esp/spr18jul2423h.spr @23:33:37.07 Gate.power :heat,:OFF @23:33:37.10 Gate.power :cartridge,:OFF @23:33:42.19 Powering cartridge bus @23:33:44.32 Gate.setIR 1750,5000,0,6 @23:33:44.45 Gate.setIR 0,5000,0,6 @23:33:44.55 Loaded Lyse-n-Go Cartridge @23:33:44.56 PuckHeater.configure HeatConfig @23:33:44.70 Gate.power :heat,:ON,limit to 5000mA @23:33:44.73 Handoff.select :waste @23:33:44.94 ArS.seek empty @23:34:02.72 PinPort.seek :cartridge @23:34:08.51 LyS1.seek 1350ul @23:34:14.52 PV.seek :reagent @23:34:15.43 AirS.seek :tVent @23:34:17.55 delay 2 seconds @23:34:19.58 AirS.reconfigure EvacAirS @23:34:19.69 AirS.seek :evac @23:34:42.07 delay 15 seconds @23:34:57.10 AirS.reconfigure AirSconfig @23:34:57.22 AirS.seek :tVent @23:35:11.48 delay 2 seconds @23:35:13.51 AirS.reconfigure EvacAirS @23:35:13.62 AirS.seek :evac @23:35:36.00 delay 15 seconds @23:35:51.03 AirS.reconfigure AirSconfig @23:35:51.15 AirS.seek :tVent @23:36:05.41 delay 2 seconds @23:36:07.44 AirS.reconfigure EvacAirS @23:36:07.54 AirS.seek :evac @23:36:29.92 delay 15 seconds @23:36:44.95 AirS.reconfigure AirSconfig @23:36:45.07 AirS.seek :tVent @23:36:59.34 delay 2 seconds @23:37:01.37 AirS.reconfigure EvacAirS @23:37:01.50 AirS.seek :evac @23:37:23.84 delay 15 seconds @23:37:38.87 delay 1:30 @23:39:08.90 AirS.reconfigure AirSconfig @23:39:09.01 AirS.seek :fVent @23:39:10.51 delay 15 seconds @23:39:25.54 LyS1.reconfigure SlowLyS1 @23:39:25.71 LyS1.seek 1050ul @23:39:46.83 delay 5 seconds @23:39:51.85 Handoff.select :blocked @23:39:52.09 LyS1.seek 450ul @23:40:34.25 delay 10 seconds @23:40:44.30 AirS.reconfigure SlowAirS @23:40:44.48 AirS.reconfigure to mix into 1.37806 parts LyS1 @23:40:45.05 AirS.seek evac @23:40:45.11 LyS1.seek 300ul @23:40:55.90 AirS.reconfigure AirSconfig @23:40:56.03 AirS.seek :full @23:41:10.31 LyS1.seek :empty @23:41:31.49 PuckHeater.seek 84.3C @23:42:13.51 PuckHeater.hold 15:00,84.3C @23:50:59.92 Session Ended @23:57:10.68 LyS1.reconfigure LyS1config @23:57:10.79 LyS1.seek 900ul @23:57:19.17 AirS.seek :tVent @23:57:19.31 delay 2 seconds @23:57:21.34 AirS.reconfigure HalfSpeedAirS @23:57:21.44 AirS.seek :evac @23:57:49.21 delay 15 seconds @23:58:04.25 AirS.reconfigure AirSconfig @23:58:04.35 AirS.seek :tVent @23:58:18.59 delay 2 seconds @23:58:20.63 AirS.reconfigure HalfSpeedAirS @23:58:20.74 AirS.seek :evac @23:58:48.51 delay 15 seconds @23:59:03.54 AirS.reconfigure AirSconfig @23:59:03.64 AirS.seek :tVent @23:59:17.88 delay 2 seconds @23:59:19.91 AirS.reconfigure HalfSpeedAirS @23:59:19.98 AirS.seek :evac @23:59:47.87 delay 15 seconds @00:00:02.90PDT25-Jul-18 delay 1:00 @00:01:03.01 Handoff.select :waste @00:01:03.14 delay 15 seconds @00:01:18.17 AirS.seek :fVent @00:01:20.42 delay 10 seconds @00:01:30.45 AirS.reconfigure AirSconfig @00:01:30.55 AirS.seek :evac @00:01:32.04 Lysis Recovered in LyS1 @00:01:32.05 Lysis Complete @00:01:32.11 Handoff.select :waste @00:01:32.19 LyS2.seek :full @00:01:32.34 LyS1.reconfigure DAmixLyS1 @00:01:32.44 LyS2.reconfigure DAmixLyS2 @00:01:33.01 LyS2.seek 1480ul @00:01:33.10 LyS1.seek 650ul @00:02:08.19 delay 5 seconds @00:02:13.22 Handoff.select :SPR @00:02:13.40 SPR.command "start 1 14" @00:02:14.31 Running Experiment-Rep #1 @00:02:14.32 delay 10 seconds @00:02:24.35 SPR.command "start 1 0" @00:02:27.67 Waiting up to 4:00 for SPR to enter state 1 @00:04:31.96 SPR.command "hold_on" @00:04:33.88 LyS2.seek 1130ul @00:04:33.91 LyS1.seek 300ul @00:05:23.00 delay 15 seconds @00:05:38.04 SPR.command "hold_off" @00:05:41.16 Waiting up to 25:00 for SPR to enter state 7 @00:25:45.98 Handoff.select :waste @00:25:46.15 LyS1.reconfigure LyS1config @00:25:46.24 LyS2.reconfigure LyS2config @00:25:46.35 Rep #1 SPR Analysis has Completed @00:25:46.91 Ended log /var/log/esp/spr18jul2423h.spr @00:25:46.93 SPR.command "stats_off" @00:25:47.95 Handoff.select :close @00:25:48.19
PV.seek :locked @00:25:49.29 ArS.seek :clear @00:25:49.48 LyS1.seek :clear @00:25:49.70 LyS2.seek :clear @00:25:49.91 AirS.seek :clear @00:26:07.08
ArS.coast @00:26:07.17 LyS1.coast @00:26:07.26 LyS2.coast @00:26:07.35 AirS.coast @00:26:07.44 TV.coast @00:26:07.53 SP.coast @00:26:07.61 SC.coast @00:26:07.70 PV.coast @00:26:07.78 PinPort.coast @00:26:08.02 Gate.power :motor,:OFF @00:26:08.04 Gate.power :heat,:OFF @00:26:08.09 Gate.power :main,:OFF @00:26:08.14 Gate.power :cartridge,:OFF @00:26:08.19 Gate.power :core,:OFF @00:26:08.24 Gate.power :analytic1,:OFF @00:26:08.29 Gate.power :analytic2,:OFF @00:26:08.32 Gate.power :analytic3,:OFF @00:26:08.38 Safely stopped and ready to power off @00:26:08.59 delay 30 seconds @00:26:38.71 Gate.power :main,:ON,limit to 5000mA @00:26:38.75 Gate.power :analytic1,:ON,limit to 5000mA @00:26:38.83 Gate.power :analytic2,:ON,limit to 5000mA @00:26:38.86 Gate.power :analytic3,:ON,limit to 5000mA @00:26:38.98 Powering core bus @00:26:41.34 SPR Module 1, FW Rev. B, FPGA Rev. C @00:26:41.53
ArS.configure ArSconfig @00:26:41.67 LyS1.configure LyS1config @00:26:41.85 LyS2.configure LyS2config @00:26:42.00 AirS.configure AirSconfig @00:26:42.18 TV.configure TVconfig @00:26:42.35 SP.configure SPconfig @00:26:42.53 SC.configure SCconfig @00:26:42.69 PV.configure PVconfig @00:26:42.86 PinPort.configure PPconfig @00:26:43.03 Sea.configure SPconfig @00:26:43.13 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @00:26:43.16 Gate.power :motor,:ON,limit to 5000mA @00:26:43.54 ArS.reconfigure ArSconfig @00:26:43.83 ArS.home.jog 0 @00:26:44.11 LyS2.reconfigure LyS2config @00:26:44.14 LyS1.reconfigure LyS1config @00:26:44.28 PV.reconfigure PVconfig @00:26:44.34 TV.reconfigure TVconfig @00:26:44.38 AirS.reconfigure AirSconfig @00:26:44.45 PinPort.reconfigure PPconfig @00:26:44.76 LyS2.home.jog 0 @00:26:44.87 LyS1.home.jog 0 @00:26:44.95 PV.home.jog 0 @00:26:45.02 TV.home.jog 0 @00:26:45.06 AirS.home.jog 0 @00:26:45.16 PinPort.home.jog 0 @00:26:45.68 ArS.reconfigure HomeMagSyringe @00:26:46.02 ArS.jog -277 @00:26:46.31 PinPort.reconfigure HomePP @00:26:46.34 PV.reconfigure HomePV @00:26:46.38 AirS.reconfigure HomeMagSyringe @00:26:46.46 LyS1.reconfigure HomeSyringe @00:26:46.54 TV.reconfigure HomeTV @00:26:46.64 LyS2.reconfigure HomeSyringe @00:26:46.93 PinPort.jog -2410 @00:26:46.99 PV.jog -1498 @00:26:47.04 LyS1.jog -273 @00:26:47.14 AirS.jog -307 @00:26:47.20 LyS2.jog -265 @00:26:47.40 TV.jog -552 @00:26:47.66 ArS.setRawPosition! -50 @00:26:47.75 ArS.seek :home @00:26:48.00 LyS1.setRawPosition! -50 @00:26:48.12 LyS1.seek :home @00:26:48.27 AirS.setRawPosition! -50 @00:26:48.31 LyS2.setRawPosition! -50 @00:26:48.37 ArS.reconfigure ArSconfig @00:26:48.57 AirS.seek :home @00:26:48.63 LyS2.seek :home @00:26:48.85 PV.setRawPosition! -50 @00:26:48.88 LyS1.reconfigure LyS1config @00:26:49.06 PV.seek :home @00:26:49.25 LyS2.reconfigure LyS2config @00:26:49.27 AirS.reconfigure AirSconfig @00:26:49.54 TV.setRawPosition! 0 @00:26:49.57 PV.reconfigure PVconfig @00:26:49.75 TV.seek :home @00:26:49.90 TV.reconfigure TVconfig @00:26:53.01 PinPort.setRawPosition! 0 @00:26:53.10 PinPort.seek :home @00:26:53.22 PinPort.reconfigure PPconfig @00:26:53.81
TV.seek :clear @00:26:55.46 TV.coast @00:26:55.55 SC.reconfigure HomeSC @00:26:55.65 SC.home.jog 0 @00:26:56.45 SC.reconfigure SCconfig @00:26:56.55 SC.seek 46 @00:26:56.69 Selecting Cartridge 43 @00:26:57.10 SC.seek 43 @00:26:58.10 LyS2.seek :locked @00:26:58.29 PV.seek :locked @00:26:58.51 AirS.seek :locked @00:27:02.64
Powering cartridge bus @00:27:04.78 Gate.setIR 1750,5000,0,6 @00:27:04.91 Gate.setIR 0,5000,0,6 @00:27:05.01 Loaded Lyse-n-Go Cartridge @00:27:05.02 PuckHeater.configure HeatConfig @00:27:05.59 Gate.power :heat,:ON,limit to 5000mA @00:27:05.91 Duration of filtering limited to 1:05:00 @00:27:06.27 Priming sample loop w/100ml, bypass w/10ml @00:27:06.39 SP.setPosition! 0ml @00:27:06.47 Intake.open @00:27:06.50 Exhaust.open @00:27:07.40 Sea @-42.9psia @00:27:07.43 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @00:27:13.20 Vacuum tare is 21% of full scale @00:27:13.22 PB.open @00:27:13.30 Exhaust.close @00:27:14.18 Sea @-42.9psia @00:27:14.32 SP.reconfigure SPprime @00:27:14.43 SP.seek 10ml,1:21 @00:27:25.34 SP.setPosition! 0ml @00:27:25.43 PB.close @00:27:25.47 Exhaust.open @00:27:26.39 Sea @-42.9psia @00:27:26.53 SP.reconfigure SPprime @00:27:26.64 SP.seek 100ml,8:51 @00:29:12.87 Pressurizing cartridge to 26psi @00:29:12.88 Intake.close @00:29:13.69 SP.setPosition! 0ml @00:29:13.78 PB.open @00:29:14.65 TV.seek :crack @00:29:15.49 TV.seek :clear @00:29:16.34 Intake.open @00:29:17.74 PB.close @00:29:18.51 Exhaust.close @00:29:19.32 Sea @-42.9psia @00:29:19.45 SP.reconfigure SPcharge @00:29:19.56 SP.seek 7.5ml,1:08 @00:29:33.28 Cartridge pressurized with 0.874ml to 26.4psi @00:29:33.76 delay 3 seconds @00:29:36.85 Cartridge at 21.8psi 3 seconds later @00:29:36.98 TV.seek :cartridge @00:29:38.83 TV.seek :clear @00:29:40.70 Sampling 500.0ml @00:29:40.72 SP.setPosition! 0ml @00:29:40.82 Exhaust.open @00:29:41.62 Sea @-42.9psia @00:29:41.77 SP.reconfigure SPsample @00:29:41.87 SP.seek 500ml,42:12 @00:29:42.79 Cartridge 43 sampling 0/500ml at 0.15ml/s with 2.6psi vacuum -39.0m deep (-42.9psia) @00:30:42.78 Cartridge 43 sampling 35/500ml at 0.58ml/s with 27.6psi vacuum -39.0m deep (-42.9psia) @00:31:42.80 Cartridge 43 sampling 70/500ml at 0.55ml/s with 21.0psi vacuum -39.0m deep (-42.9psia) @00:32:42.79 Cartridge 43 sampling 104/500ml at 0.56ml/s with 20.7psi vacuum -39.0m deep (-42.9psia) @00:33:42.79 Cartridge 43 sampling 138/500ml at 0.58ml/s with 17.0psi vacuum -39.0m deep (-42.9psia) @00:34:42.79 Cartridge 43 sampling 172/500ml at 0.55ml/s with 17.4psi vacuum -39.0m deep (-42.9psia) @00:35:42.79 Cartridge 43 sampling 206/500ml at 0.57ml/s with 27.5psi vacuum -39.0m deep (-42.9psia) @00:36:42.79 Cartridge 43 sampling 239/500ml at 0.56ml/s with 24.8psi vacuum -39.0m deep (-42.9psia) @00:37:42.78 Cartridge 43 sampling 272/500ml at 0.51ml/s with 22.9psi vacuum -39.0m deep (-42.9psia) @00:38:42.80 Cartridge 43 sampling 304/500ml at 0.54ml/s with 24.3psi vacuum -39.0m deep (-42.9psia) @00:39:42.80 Cartridge 43 sampling 335/500ml at 0.50ml/s with 20.3psi vacuum -39.0m deep (-42.9psia) @00:40:42.80 Cartridge 43 sampling 365/500ml at 0.53ml/s with 23.4psi vacuum -39.0m deep (-42.9psia) @00:41:42.80 Cartridge 43 sampling 395/500ml at 0.51ml/s with 22.4psi vacuum -39.0m deep (-42.9psia) @00:42:42.78 Cartridge 43 sampling 424/500ml at 0.49ml/s with 21.2psi vacuum -39.0m deep (-42.9psia) @00:43:42.80 Cartridge 43 sampling 453/500ml at 0.46ml/s with 17.9psi vacuum -39.0m deep (-42.9psia) @00:44:42.81 Cartridge 43 sampling 482/500ml at 0.47ml/s with 24.0psi vacuum -39.0m deep (-42.9psia) @00:45:22.06 Intake.close @00:45:22.11 Exhaust.close @00:45:22.97 Sampled 500.0ml @00:45:23.10 TV.seek :bypass @00:45:25.22 TV.seek :clear @00:45:28.75 SPR.command "read_sequences" @00:45:28.82 SPR.command "stats_on" @00:45:29.62 SPR.command "start 2 0" @00:45:31.62 Begun log /var/log/esp/spr18jul2500h.spr @00:45:32.86 Gate.power :heat,:OFF @00:45:32.88 Gate.power :cartridge,:OFF @00:45:37.97 Powering cartridge bus @00:45:40.10 Gate.setIR 1750,5000,0,6 @00:45:40.25 Gate.setIR 0,5000,0,6 @00:45:40.34 Loaded Lyse-n-Go Cartridge @00:45:40.36 PuckHeater.configure HeatConfig @00:45:40.46 Gate.power :heat,:ON,limit to 5000mA @00:45:40.49 Handoff.select :waste @00:45:40.69 ArS.seek empty @00:45:58.46 PinPort.seek :cartridge @00:46:04.28 LyS1.seek 1350ul @00:46:10.27 PV.seek :reagent @00:46:11.17 AirS.seek :tVent @00:46:13.29 delay 2 seconds @00:46:15.32 AirS.reconfigure EvacAirS @00:46:15.43 AirS.seek :evac @00:46:37.79 delay 15 seconds @00:46:52.82 AirS.reconfigure AirSconfig @00:46:52.92 AirS.seek :tVent @00:47:07.16 delay 2 seconds @00:47:09.19 AirS.reconfigure EvacAirS @00:47:09.30 AirS.seek :evac @00:47:31.62 delay 15 seconds @00:47:46.65 AirS.reconfigure AirSconfig @00:47:46.77 AirS.seek :tVent @00:48:01.04 delay 2 seconds @00:48:03.07 AirS.reconfigure EvacAirS @00:48:03.17 AirS.seek :evac @00:48:25.53 delay 15 seconds @00:48:40.56 AirS.reconfigure AirSconfig @00:48:40.66 AirS.seek :tVent @00:48:54.90 delay 2 seconds @00:48:56.93 AirS.reconfigure EvacAirS @00:48:57.03 AirS.seek :evac @00:49:19.37 delay 15 seconds @00:49:34.40 delay 1:30 @00:51:04.43 AirS.reconfigure AirSconfig @00:51:04.52 AirS.seek :fVent @00:51:06.00 delay 15 seconds @00:51:21.04 LyS1.reconfigure SlowLyS1 @00:51:21.20 LyS1.seek 1050ul @00:51:42.31 delay 5 seconds @00:51:47.33 Handoff.select :blocked @00:51:47.58 LyS1.seek 450ul @00:52:29.63 delay 10 seconds @00:52:39.66 AirS.reconfigure SlowAirS @00:52:39.81 AirS.reconfigure to mix into 1.37806 parts LyS1 @00:52:40.36 AirS.seek evac @00:52:40.41 LyS1.seek 300ul @00:52:51.19 AirS.reconfigure AirSconfig @00:52:51.31 AirS.seek :full @00:53:05.57 LyS1.seek :empty @00:53:26.73 PuckHeater.seek 84.3C @00:54:04.34 PuckHeater.hold 15:00,84.3C @01:09:02.55 LyS1.reconfigure LyS1config @01:09:02.66 LyS1.seek 900ul @01:09:11.06 AirS.seek :tVent @01:09:11.19 delay 2 seconds @01:09:13.23 AirS.reconfigure HalfSpeedAirS @01:09:13.34 AirS.seek :evac @01:09:41.13 delay 15 seconds @01:09:56.17 AirS.reconfigure AirSconfig @01:09:56.28 AirS.seek :tVent @01:10:10.54 delay 2 seconds @01:10:12.57 AirS.reconfigure HalfSpeedAirS @01:10:12.67 AirS.seek :evac @01:10:40.45 delay 15 seconds @01:10:55.48 AirS.reconfigure AirSconfig @01:10:55.59 AirS.seek :tVent @01:11:09.85 delay 2 seconds @01:11:11.88 AirS.reconfigure HalfSpeedAirS @01:11:11.98 AirS.seek :evac @01:11:39.77 delay 15 seconds @01:11:54.80 delay 1:00 @01:12:54.81 Handoff.select :waste @01:12:54.98 delay 15 seconds @01:13:10.02 AirS.seek :fVent @01:13:12.27 delay 10 seconds @01:13:22.30 AirS.reconfigure AirSconfig @01:13:22.42 AirS.seek :evac @01:13:23.90 Lysis Recovered in LyS1 @01:13:23.92 Lysis Complete @01:13:23.95 Handoff.select :waste @01:13:24.02 LyS2.seek :full @01:13:24.17 LyS1.reconfigure DAmixLyS1 @01:13:24.29 LyS2.reconfigure DAmixLyS2 @01:13:24.85 LyS2.seek 1480ul @01:13:24.90 LyS1.seek 650ul @01:14:00.03 delay 5 seconds @01:14:05.05 Handoff.select :SPR @01:14:05.20 SPR.command "start 1 14" @01:14:06.30 Running Experiment-Rep #1 @01:14:06.31 delay 10 seconds @01:14:16.36 SPR.command "start 1 0" @01:14:19.66 Waiting up to 4:00 for SPR to enter state 1 @01:16:23.95 SPR.command "hold_on" @01:16:25.86 LyS2.seek 1130ul @01:16:25.89 LyS1.seek 300ul @01:17:14.97 delay 15 seconds @01:17:30.00 SPR.command "hold_off" @01:17:33.14 Waiting up to 25:00 for SPR to enter state 7 @01:37:37.97 Handoff.select :waste @01:37:38.14 LyS1.reconfigure LyS1config @01:37:38.23 LyS2.reconfigure LyS2config @01:37:38.32 Rep #1 SPR Analysis has Completed @01:37:38.90 Ended log /var/log/esp/spr18jul2500h.spr @01:37:38.92 SPR.command "stats_off" @01:37:39.43 Handoff.select :close @01:37:39.65
PV.seek :locked @01:37:40.66 ArS.seek :clear @01:37:40.85 LyS1.seek :clear @01:37:41.05 LyS2.seek :clear @01:37:41.26 AirS.seek :clear @01:37:58.47
ArS.coast @01:37:58.56 LyS1.coast @01:37:58.64 LyS2.coast @01:37:58.72 AirS.coast @01:37:58.81 TV.coast @01:37:58.89 SP.coast @01:37:58.98 SC.coast @01:37:59.07 PV.coast @01:37:59.14 PinPort.coast @01:37:59.39 Gate.power :motor,:OFF @01:37:59.41 Gate.power :heat,:OFF @01:37:59.46 Gate.power :main,:OFF @01:37:59.50 Gate.power :cartridge,:OFF @01:37:59.54 Gate.power :core,:OFF @01:37:59.59 Gate.power :analytic1,:OFF @01:37:59.65 Gate.power :analytic2,:OFF @01:37:59.70 Gate.power :analytic3,:OFF @01:37:59.74 Safely stopped and ready to power off @01:37:59.96 delay 30 seconds @01:38:30.09 Gate.power :main,:ON,limit to 5000mA @01:38:30.13 Gate.power :analytic1,:ON,limit to 5000mA @01:38:30.18 Gate.power :analytic2,:ON,limit to 5000mA @01:38:30.24 Gate.power :analytic3,:ON,limit to 5000mA @01:38:30.35 Powering core bus @01:38:32.72 SPR Module 1, FW Rev. B, FPGA Rev. C @01:38:32.90
ArS.configure ArSconfig @01:38:33.06 LyS1.configure LyS1config @01:38:33.23 LyS2.configure LyS2config @01:38:33.38 AirS.configure AirSconfig @01:38:33.56 TV.configure TVconfig @01:38:33.73 SP.configure SPconfig @01:38:33.91 SC.configure SCconfig @01:38:34.09 PV.configure PVconfig @01:38:34.27 PinPort.configure PPconfig @01:38:34.44 Sea.configure SPconfig @01:38:34.54 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @01:38:34.57 Gate.power :motor,:ON,limit to 5000mA @01:38:34.86 ArS.reconfigure ArSconfig @01:38:35.27 ArS.home.jog 0 @01:38:35.53 PV.reconfigure PVconfig @01:38:35.57 TV.reconfigure TVconfig @01:38:35.61 AirS.reconfigure AirSconfig @01:38:35.72 PinPort.reconfigure PPconfig @01:38:35.79 LyS2.reconfigure LyS2config @01:38:35.83 LyS1.reconfigure LyS1config @01:38:36.21 PV.home.jog 0 @01:38:36.25 TV.home.jog 0 @01:38:36.29 AirS.home.jog 0 @01:38:36.36 LyS2.home.jog 0 @01:38:36.43 PinPort.home.jog 0 @01:38:36.50 LyS1.home.jog 0 @01:38:37.07 ArS.reconfigure HomeMagSyringe @01:38:37.42 ArS.jog -278 @01:38:37.63 AirS.reconfigure HomeMagSyringe @01:38:37.71 PinPort.reconfigure HomePP @01:38:37.79 TV.reconfigure HomeTV @01:38:37.83 LyS2.reconfigure HomeSyringe @01:38:37.92 LyS1.reconfigure HomeSyringe @01:38:38.01 PV.reconfigure HomePV @01:38:38.32 AirS.jog -307 @01:38:38.37 TV.jog -547 @01:38:38.41 PinPort.jog -2410 @01:38:38.48 LyS2.jog -265 @01:38:38.55 LyS1.jog -274 @01:38:38.62 PV.jog -1499 @01:38:38.96 ArS.setRawPosition! -50 @01:38:39.05 ArS.seek :home @01:38:39.28 AirS.setRawPosition! -50 @01:38:39.39 AirS.seek :home @01:38:39.54 LyS1.setRawPosition! -50 @01:38:39.58 ArS.reconfigure ArSconfig @01:38:39.64 LyS2.setRawPosition! -50 @01:38:39.82 LyS1.seek :home @01:38:39.90 LyS2.seek :home @01:38:40.14 AirS.reconfigure AirSconfig @01:38:40.19 PV.setRawPosition! -50 @01:38:40.39 PV.seek :home @01:38:40.53 LyS1.reconfigure LyS1config @01:38:40.55 LyS2.reconfigure LyS2config @01:38:40.80 TV.setRawPosition! 0 @01:38:40.83 PV.reconfigure PVconfig @01:38:40.97 TV.seek :home @01:38:41.12 TV.reconfigure TVconfig @01:38:44.50 PinPort.setRawPosition! 0 @01:38:44.59 PinPort.seek :home @01:38:44.72 PinPort.reconfigure PPconfig @01:38:45.30
TV.seek :clear @01:38:46.94 TV.coast @01:38:47.04 SC.reconfigure HomeSC @01:38:47.14 SC.home.jog 0 @01:38:47.94 SC.reconfigure SCconfig @01:38:48.04 SC.seek 43 @01:38:48.17 Selecting Cartridge 40 @01:38:48.59 SC.seek 40 @01:38:49.59 LyS2.seek :locked @01:38:49.78 PV.seek :locked @01:38:49.99 AirS.seek :locked @01:38:54.12
Powering cartridge bus @01:38:56.26 Gate.setIR 1750,5000,0,6 @01:38:56.39 Gate.setIR 0,5000,0,6 @01:38:56.48 Loaded Lyse-n-Go Cartridge @01:38:56.50 PuckHeater.configure HeatConfig @01:38:57.06 Gate.power :heat,:ON,limit to 5000mA @01:38:57.38 Duration of filtering limited to 1:05:00 @01:38:57.73 Priming sample loop w/100ml, bypass w/10ml @01:38:57.86 SP.setPosition! 0ml @01:38:57.94 Intake.open @01:38:57.98 Exhaust.open @01:38:58.90 Sea @-42.9psia @01:38:58.92 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @01:39:04.70 Vacuum tare is 20% of full scale @01:39:04.72 PB.open @01:39:04.75 Exhaust.close @01:39:05.68 Sea @-42.9psia @01:39:05.82 SP.reconfigure SPprime @01:39:05.92 SP.seek 10ml,1:21 @01:39:16.85 SP.setPosition! 0ml @01:39:16.93 PB.close @01:39:17.00 Exhaust.open @01:39:17.90 Sea @-42.9psia @01:39:18.04 SP.reconfigure SPprime @01:39:18.15 SP.seek 100ml,8:51 @01:41:04.86 Pressurizing cartridge to 26psi @01:41:04.87 Intake.close @01:41:05.68 SP.setPosition! 0ml @01:41:05.73 PB.open @01:41:06.58 TV.seek :crack @01:41:07.42 TV.seek :clear @01:41:08.27 Intake.open @01:41:09.72 PB.close @01:41:10.45 Exhaust.close @01:41:11.24 Sea @-42.9psia @01:41:11.38 SP.reconfigure SPcharge @01:41:11.48 SP.seek 7.5ml,1:08 @01:41:25.36 Cartridge pressurized with 0.896ml to 26.2psi @01:41:25.68 delay 3 seconds @01:41:28.77 Cartridge at 21.5psi 3 seconds later @01:41:28.91 TV.seek :cartridge @01:41:30.77 TV.seek :clear @01:41:32.64 Sampling 500.0ml @01:41:32.66 SP.setPosition! 0ml @01:41:32.77 Exhaust.open @01:41:33.59 Sea @-42.9psia @01:41:33.74 SP.reconfigure SPsample @01:41:33.84 SP.seek 500ml,42:12 @01:41:34.76 Cartridge 40 sampling 0/500ml at 0.15ml/s with 3.0psi vacuum -39.0m deep (-42.9psia) @01:42:34.76 Cartridge 40 sampling 34/500ml at 0.59ml/s with 20.4psi vacuum -39.0m deep (-42.9psia) @01:43:34.76 Cartridge 40 sampling 68/500ml at 0.57ml/s with 23.0psi vacuum -39.0m deep (-42.9psia) @01:44:34.75 Cartridge 40 sampling 102/500ml at 0.55ml/s with 23.3psi vacuum -39.0m deep (-42.9psia) @01:45:34.75 Cartridge 40 sampling 135/500ml at 0.56ml/s with 20.9psi vacuum -39.0m deep (-42.9psia) @01:46:34.75 Cartridge 40 sampling 167/500ml at 0.55ml/s with 24.9psi vacuum -39.0m deep (-42.9psia) @01:47:34.76 Cartridge 40 sampling 200/500ml at 0.53ml/s with 22.6psi vacuum -39.0m deep (-42.9psia) @01:48:34.76 Cartridge 40 sampling 233/500ml at 0.57ml/s with 21.5psi vacuum -39.0m deep (-42.9psia) @01:49:34.75 Cartridge 40 sampling 265/500ml at 0.53ml/s with 21.0psi vacuum -39.0m deep (-42.9psia) @01:50:34.76 Cartridge 40 sampling 298/500ml at 0.53ml/s with 28.3psi vacuum -39.0m deep (-42.9psia) @01:51:34.76 Cartridge 40 sampling 330/500ml at 0.55ml/s with 25.4psi vacuum -39.0m deep (-42.9psia) @01:52:34.75 Cartridge 40 sampling 361/500ml at 0.53ml/s with 27.4psi vacuum -39.0m deep (-42.9psia) @01:53:34.75 Cartridge 40 sampling 392/500ml at 0.52ml/s with 15.1psi vacuum -39.0m deep (-42.9psia) @01:54:34.76 Cartridge 40 sampling 422/500ml at 0.50ml/s with 22.6psi vacuum -39.0m deep (-42.9psia) @01:55:34.76 Cartridge 40 sampling 452/500ml at 0.46ml/s with 26.0psi vacuum -39.0m deep (-42.9psia) @01:56:34.75 Cartridge 40 sampling 481/500ml at 0.48ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @01:57:17.46 Intake.close @01:57:17.53 Exhaust.close @01:57:18.37 Sampled 500.0ml @01:57:18.51 TV.seek :bypass @01:57:20.61 TV.seek :clear @01:57:24.15 SPR.command "read_sequences" @01:57:24.77 SPR.command "stats_on" @01:57:24.85 SPR.command "start 2 0" @01:57:26.85 Begun log /var/log/esp/spr18jul2501h.spr @01:57:28.03 Gate.power :heat,:OFF @01:57:28.08 Gate.power :cartridge,:OFF @01:57:33.15 Powering cartridge bus @01:57:35.29 Gate.setIR 1750,5000,0,6 @01:57:35.42 Gate.setIR 0,5000,0,6 @01:57:35.51 Loaded Lyse-n-Go Cartridge @01:57:35.53 PuckHeater.configure HeatConfig @01:57:35.63 Gate.power :heat,:ON,limit to 5000mA @01:57:35.67 Handoff.select :waste @01:57:35.87 ArS.seek empty @01:57:53.64 PinPort.seek :cartridge @01:57:59.43 LyS1.seek 1350ul @01:58:05.43 PV.seek :reagent @01:58:06.32 AirS.seek :tVent @01:58:08.44 delay 2 seconds @01:58:10.47 AirS.reconfigure EvacAirS @01:58:10.57 AirS.seek :evac @01:58:32.96 delay 15 seconds @01:58:47.99 AirS.reconfigure AirSconfig @01:58:48.09 AirS.seek :tVent @01:59:02.36 delay 2 seconds @01:59:04.39 AirS.reconfigure EvacAirS @01:59:04.50 AirS.seek :evac @01:59:26.85 delay 15 seconds @01:59:41.88 AirS.reconfigure AirSconfig @01:59:41.98 AirS.seek :tVent @01:59:56.23 delay 2 seconds @01:59:58.26 AirS.reconfigure EvacAirS @01:59:58.36 AirS.seek :evac @02:00:20.71 delay 15 seconds @02:00:35.74 AirS.reconfigure AirSconfig @02:00:35.84 AirS.seek :tVent @02:00:50.09 delay 2 seconds @02:00:52.13 AirS.reconfigure EvacAirS @02:00:52.23 AirS.seek :evac @02:01:14.58 delay 15 seconds @02:01:29.61 delay 1:30 @02:02:59.64 AirS.reconfigure AirSconfig @02:02:59.74 AirS.seek :fVent @02:03:01.24 delay 15 seconds @02:03:16.27 LyS1.reconfigure SlowLyS1 @02:03:16.42 LyS1.seek 1050ul @02:03:37.53 delay 5 seconds @02:03:42.55 Handoff.select :blocked @02:03:42.79 LyS1.seek 450ul @02:04:24.88 delay 10 seconds @02:04:34.91 AirS.reconfigure SlowAirS @02:04:35.06 AirS.reconfigure to mix into 1.37806 parts LyS1 @02:04:35.60 AirS.seek evac @02:04:35.66 LyS1.seek 300ul @02:04:46.42 AirS.reconfigure AirSconfig @02:04:46.52 AirS.seek :full @02:05:00.79 LyS1.seek :empty @02:05:21.98 PuckHeater.seek 84.3C @02:06:12.24 PuckHeater.hold 15:00,84.3C @02:21:09.01 LyS1.reconfigure LyS1config @02:21:09.12 LyS1.seek 900ul @02:21:17.50 AirS.seek :tVent @02:21:17.65 delay 2 seconds @02:21:19.68 AirS.reconfigure HalfSpeedAirS @02:21:19.78 AirS.seek :evac @02:21:47.57 delay 15 seconds @02:22:02.60 AirS.reconfigure AirSconfig @02:22:02.72 AirS.seek :tVent @02:22:16.98 delay 2 seconds @02:22:19.01 AirS.reconfigure HalfSpeedAirS @02:22:19.11 AirS.seek :evac @02:22:46.87 delay 15 seconds @02:23:01.90 AirS.reconfigure AirSconfig @02:23:02.01 AirS.seek :tVent @02:23:16.27 delay 2 seconds @02:23:18.31 AirS.reconfigure HalfSpeedAirS @02:23:18.42 AirS.seek :evac @02:23:46.30 delay 15 seconds @02:24:01.33 delay 1:00 @02:25:01.35 Handoff.select :waste @02:25:01.54 delay 15 seconds @02:25:16.57 AirS.seek :fVent @02:25:18.80 delay 10 seconds @02:25:28.84 AirS.reconfigure AirSconfig @02:25:28.94 AirS.seek :evac @02:25:30.43 Lysis Recovered in LyS1 @02:25:30.44 Lysis Complete @02:25:30.49 Handoff.select :waste @02:25:30.56 LyS2.seek :full @02:25:30.71 LyS1.reconfigure DAmixLyS1 @02:25:30.83 LyS2.reconfigure DAmixLyS2 @02:25:31.40 LyS2.seek 1480ul @02:25:31.52 LyS1.seek 650ul @02:26:06.59 delay 5 seconds @02:26:11.61 Handoff.select :SPR @02:26:11.77 SPR.command "start 1 14" @02:26:12.82 Running Experiment-Rep #1 @02:26:12.83 delay 10 seconds @02:26:22.86 SPR.command "start 1 0" @02:26:26.17 Waiting up to 4:00 for SPR to enter state 1 @02:28:30.45 SPR.command "hold_on" @02:28:32.36 LyS2.seek 1130ul @02:28:32.42 LyS1.seek 300ul @02:29:21.51 delay 15 seconds @02:29:36.54 SPR.command "hold_off" @02:29:39.11 Waiting up to 25:00 for SPR to enter state 7 @02:49:43.52 Handoff.select :waste @02:49:43.70 LyS1.reconfigure LyS1config @02:49:43.79 LyS2.reconfigure LyS2config @02:49:43.88 Rep #1 SPR Analysis has Completed @02:49:44.46 Ended log /var/log/esp/spr18jul2501h.spr @02:49:44.48 SPR.command "stats_off" @02:49:45.00 Handoff.select :close @02:49:45.26
PV.seek :locked @02:49:46.29 ArS.seek :clear @02:49:46.48 LyS1.seek :clear @02:49:46.70 LyS2.seek :clear @02:49:46.89 AirS.seek :clear @02:50:04.09
ArS.coast @02:50:04.19 LyS1.coast @02:50:04.28 LyS2.coast @02:50:04.36 AirS.coast @02:50:04.44 TV.coast @02:50:04.53 SP.coast @02:50:04.61 SC.coast @02:50:04.70 PV.coast @02:50:04.78 PinPort.coast @02:50:05.00 Gate.power :motor,:OFF @02:50:05.02 Gate.power :heat,:OFF @02:50:05.07 Gate.power :main,:OFF @02:50:05.12 Gate.power :cartridge,:OFF @02:50:05.16 Gate.power :core,:OFF @02:50:05.21 Gate.power :analytic1,:OFF @02:50:05.26 Gate.power :analytic2,:OFF @02:50:05.31 Gate.power :analytic3,:OFF @02:50:05.36 Safely stopped and ready to power off @02:50:05.57 delay 30 seconds @02:50:35.69 Gate.power :main,:ON,limit to 5000mA @02:50:35.73 Gate.power :analytic1,:ON,limit to 5000mA @02:50:35.81 Gate.power :analytic2,:ON,limit to 5000mA @02:50:35.83 Gate.power :analytic3,:ON,limit to 5000mA @02:50:35.95 Powering core bus @02:50:38.29 SPR Module 1, FW Rev. B, FPGA Rev. C @02:50:38.50
ArS.configure ArSconfig @02:50:38.65 LyS1.configure LyS1config @02:50:38.82 LyS2.configure LyS2config @02:50:38.97 AirS.configure AirSconfig @02:50:39.14 TV.configure TVconfig @02:50:39.32 SP.configure SPconfig @02:50:39.50 SC.configure SCconfig @02:50:39.67 PV.configure PVconfig @02:50:39.84 PinPort.configure PPconfig @02:50:40.01 Sea.configure SPconfig @02:50:40.11 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @02:50:40.13 Gate.power :motor,:ON,limit to 5000mA @02:50:40.48 ArS.reconfigure ArSconfig @02:50:40.84 ArS.home.jog 0 @02:50:41.08 PV.reconfigure PVconfig @02:50:41.13 TV.reconfigure TVconfig @02:50:41.21 PinPort.reconfigure PPconfig @02:50:41.31 LyS2.reconfigure LyS2config @02:50:41.38 LyS1.reconfigure LyS1config @02:50:41.47 AirS.reconfigure AirSconfig @02:50:41.74 PV.home.jog 0 @02:50:41.79 TV.home.jog 0 @02:50:41.88 PinPort.home.jog 0 @02:50:41.93 LyS2.home.jog 0 @02:50:41.97 LyS1.home.jog 0 @02:50:42.04 AirS.home.jog 0 @02:50:42.52 ArS.reconfigure HomeMagSyringe @02:50:42.80 ArS.jog -277 @02:50:43.03 PinPort.reconfigure HomePP @02:50:43.09 PV.reconfigure HomePV @02:50:43.15 LyS2.reconfigure HomeSyringe @02:50:43.27 TV.reconfigure HomeTV @02:50:43.56 PinPort.jog -2410 @02:50:43.61 PV.jog -1500 @02:50:43.66 LyS2.jog -266 @02:50:43.74 TV.jog -548 @02:50:44.05 ArS.setRawPosition! -50 @02:50:44.09 LyS1.reconfigure HomeSyringe @02:50:44.15 AirS.reconfigure HomeMagSyringe @02:50:44.35 ArS.seek :home @02:50:44.46 LyS1.jog -274 @02:50:44.57 AirS.jog -308 @02:50:44.77 LyS2.setRawPosition! -50 @02:50:44.87 LyS2.seek :home @02:50:44.99 ArS.reconfigure ArSconfig @02:50:45.11 PV.setRawPosition! -50 @02:50:45.23 PV.seek :home @02:50:45.38 AirS.setRawPosition! -50 @02:50:45.42 LyS2.reconfigure LyS2config @02:50:45.47 LyS1.setRawPosition! -50 @02:50:45.67 AirS.seek :home @02:50:45.75 LyS1.seek :home @02:50:46.00 TV.setRawPosition! 0 @02:50:46.03 PV.reconfigure PVconfig @02:50:46.25 TV.seek :home @02:50:46.40 TV.reconfigure TVconfig @02:50:46.44 AirS.reconfigure AirSconfig @02:50:46.48 LyS1.reconfigure LyS1config @02:50:49.53 PinPort.setRawPosition! 0 @02:50:49.62 PinPort.seek :home @02:50:49.74 PinPort.reconfigure PPconfig @02:50:50.32
TV.seek :clear @02:50:51.97 TV.coast @02:50:52.07 SC.reconfigure HomeSC @02:50:52.17 SC.home.jog 0 @02:50:52.99 SC.reconfigure SCconfig @02:50:53.08 SC.seek 40 @02:50:53.20 Selecting Cartridge 37 @02:50:53.61 SC.seek 37 @02:50:54.58 LyS2.seek :locked @02:50:54.78 PV.seek :locked @02:50:54.98 AirS.seek :locked @02:50:59.12
Powering cartridge bus @02:51:01.25 Gate.setIR 1750,5000,0,6 @02:51:01.38 Gate.setIR 0,5000,0,6 @02:51:01.48 Loaded Lyse-n-Go Cartridge @02:51:01.49 PuckHeater.configure HeatConfig @02:51:02.05 Gate.power :heat,:ON,limit to 5000mA @02:51:02.36 Duration of filtering limited to 1:05:00 @02:51:02.72 Priming sample loop w/100ml, bypass w/10ml @02:51:02.85 SP.setPosition! 0ml @02:51:02.90 Intake.open @02:51:02.96 Exhaust.open @02:51:03.84 Sea @-42.9psia @02:51:03.86 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @02:51:09.60 Vacuum tare is 20% of full scale @02:51:09.62 PB.open @02:51:09.69 Exhaust.close @02:51:10.55 Sea @-42.9psia @02:51:10.69 SP.reconfigure SPprime @02:51:10.80 SP.seek 10ml,1:21 @02:51:21.72 SP.setPosition! 0ml @02:51:21.81 PB.close @02:51:21.86 Exhaust.open @02:51:22.74 Sea @-42.9psia @02:51:22.88 SP.reconfigure SPprime @02:51:22.99 SP.seek 100ml,8:51 @02:53:09.31 Pressurizing cartridge to 26psi @02:53:09.33 Intake.close @02:53:10.14 SP.setPosition! 0ml @02:53:10.20 PB.open @02:53:11.03 TV.seek :crack @02:53:11.87 TV.seek :clear @02:53:12.71 Intake.open @02:53:14.18 PB.close @02:53:14.94 Exhaust.close @02:53:15.73 Sea @-42.9psia @02:53:15.87 SP.reconfigure SPcharge @02:53:15.98 SP.seek 7.5ml,1:08 @02:53:29.69 Cartridge pressurized with 0.875ml to 26.0psi @02:53:30.00 delay 3 seconds @02:53:33.08 Cartridge at 19.7psi 3 seconds later @02:53:33.21 TV.seek :cartridge @02:53:35.08 TV.seek :clear @02:53:36.95 Sampling 500.0ml @02:53:36.97 SP.setPosition! 0ml @02:53:37.08 Exhaust.open @02:53:37.93 Sea @-42.9psia @02:53:38.08 SP.reconfigure SPsample @02:53:38.19 SP.seek 500ml,42:12 @02:53:39.09 Cartridge 37 sampling 0/500ml at 0.15ml/s with 2.9psi vacuum -39.0m deep (-42.9psia) @02:54:39.11 Cartridge 37 sampling 35/500ml at 0.59ml/s with 21.9psi vacuum -39.0m deep (-42.9psia) @02:55:39.10 Cartridge 37 sampling 70/500ml at 0.57ml/s with 21.0psi vacuum -39.0m deep (-42.9psia) @02:56:39.11 Cartridge 37 sampling 104/500ml at 0.54ml/s with 25.5psi vacuum -39.0m deep (-42.9psia) @02:57:39.10 Cartridge 37 sampling 137/500ml at 0.56ml/s with 21.0psi vacuum -39.0m deep (-42.9psia) @02:58:39.10 Cartridge 37 sampling 171/500ml at 0.54ml/s with 18.4psi vacuum -39.0m deep (-42.9psia) @02:59:39.10 Cartridge 37 sampling 204/500ml at 0.53ml/s with 17.9psi vacuum -39.0m deep (-42.9psia) @03:00:39.10 Cartridge 37 sampling 238/500ml at 0.58ml/s with 24.4psi vacuum -39.0m deep (-42.9psia) @03:01:39.10 Cartridge 37 sampling 271/500ml at 0.58ml/s with 25.3psi vacuum -39.0m deep (-42.9psia) @03:02:39.11 Cartridge 37 sampling 305/500ml at 0.52ml/s with 21.7psi vacuum -39.0m deep (-42.9psia) @03:03:39.10 Cartridge 37 sampling 337/500ml at 0.55ml/s with 15.2psi vacuum -39.0m deep (-42.9psia) @03:04:39.11 Cartridge 37 sampling 369/500ml at 0.54ml/s with 17.4psi vacuum -39.0m deep (-42.9psia) @03:05:39.10 Cartridge 37 sampling 401/500ml at 0.51ml/s with 25.2psi vacuum -39.0m deep (-42.9psia) @03:06:39.10 Cartridge 37 sampling 432/500ml at 0.53ml/s with 18.7psi vacuum -39.0m deep (-42.9psia) @03:07:39.10 Cartridge 37 sampling 463/500ml at 0.48ml/s with 21.0psi vacuum -39.0m deep (-42.9psia) @03:08:39.10 Cartridge 37 sampling 493/500ml at 0.48ml/s with 25.3psi vacuum -39.0m deep (-42.9psia) @03:08:58.25 Intake.close @03:08:58.30 Exhaust.close @03:08:59.16 Sampled 500.0ml @03:08:59.30 TV.seek :bypass @03:09:01.39 TV.seek :clear @03:09:04.91 SPR.command "read_sequences" @03:09:04.98 SPR.command "stats_on" @03:09:05.77 SPR.command "start 2 0" @03:09:07.77 Begun log /var/log/esp/spr18jul2503h.spr AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @03:09:10.89 Gate.power :heat,:OFF @03:09:10.94 Gate.power :cartridge,:OFF @03:09:16.01 Powering cartridge bus @03:09:18.14 Gate.setIR 1750,5000,0,6 @03:09:18.27 Gate.setIR 0,5000,0,6 @03:09:18.37 Loaded Lyse-n-Go Cartridge @03:09:18.38 PuckHeater.configure HeatConfig @03:09:18.50 Gate.power :heat,:ON,limit to 5000mA @03:09:18.52 Handoff.select :waste @03:09:18.73 ArS.seek empty @03:09:36.49 PinPort.seek :cartridge @03:09:42.30 LyS1.seek 1350ul @03:09:48.29 PV.seek :reagent @03:09:49.18 AirS.seek :tVent @03:09:51.30 delay 2 seconds @03:09:53.33 AirS.reconfigure EvacAirS @03:09:53.44 AirS.seek :evac @03:10:15.80 delay 15 seconds @03:10:30.83 AirS.reconfigure AirSconfig @03:10:30.93 AirS.seek :tVent @03:10:45.20 delay 2 seconds @03:10:47.24 AirS.reconfigure EvacAirS @03:10:47.34 AirS.seek :evac @03:11:09.70 delay 15 seconds @03:11:24.73 AirS.reconfigure AirSconfig @03:11:24.83 AirS.seek :tVent @03:11:39.10 delay 2 seconds @03:11:41.13 AirS.reconfigure EvacAirS @03:11:41.23 AirS.seek :evac @03:12:03.59 delay 15 seconds @03:12:18.62 AirS.reconfigure AirSconfig @03:12:18.73 AirS.seek :tVent @03:12:32.99 delay 2 seconds @03:12:35.02 AirS.reconfigure EvacAirS @03:12:35.12 AirS.seek :evac @03:12:57.47 delay 15 seconds @03:13:12.50 delay 1:30 @03:14:42.54 AirS.reconfigure AirSconfig @03:14:42.64 AirS.seek :fVent @03:14:44.12 delay 15 seconds @03:14:59.16 LyS1.reconfigure SlowLyS1 @03:14:59.31 LyS1.seek 1050ul @03:15:20.43 delay 5 seconds @03:15:25.45 Handoff.select :blocked @03:15:25.68 LyS1.seek 450ul @03:16:07.81 delay 10 seconds @03:16:17.84 AirS.reconfigure SlowAirS @03:16:18.01 AirS.reconfigure to mix into 1.37806 parts LyS1 @03:16:18.55 AirS.seek evac @03:16:18.66 LyS1.seek 300ul @03:16:29.34 AirS.reconfigure AirSconfig @03:16:29.44 AirS.seek :full @03:16:43.70 LyS1.seek :empty @03:17:04.89 PuckHeater.seek 84.3C @03:17:41.33 PuckHeater.hold 15:00,84.3C @03:32:42.47 LyS1.reconfigure LyS1config @03:32:42.58 LyS1.seek 900ul @03:32:50.98 AirS.seek :tVent @03:32:51.11 delay 2 seconds @03:32:53.14 AirS.reconfigure HalfSpeedAirS @03:32:53.25 AirS.seek :evac @03:33:21.04 delay 15 seconds @03:33:36.09 AirS.reconfigure AirSconfig @03:33:36.19 AirS.seek :tVent @03:33:50.45 delay 2 seconds @03:33:52.48 AirS.reconfigure HalfSpeedAirS @03:33:52.59 AirS.seek :evac @03:34:20.35 delay 15 seconds @03:34:35.38 AirS.reconfigure AirSconfig @03:34:35.49 AirS.seek :tVent @03:34:49.75 delay 2 seconds @03:34:51.78 AirS.reconfigure HalfSpeedAirS @03:34:51.88 AirS.seek :evac @03:35:19.67 delay 15 seconds @03:35:34.70 delay 1:00 @03:36:34.72 Handoff.select :waste @03:36:35.00 delay 15 seconds @03:36:50.03 AirS.seek :fVent @03:36:52.28 delay 10 seconds @03:37:02.31 AirS.reconfigure AirSconfig @03:37:02.41 AirS.seek :evac @03:37:03.90 Lysis Recovered in LyS1 @03:37:03.92 Lysis Complete @03:37:03.96 Handoff.select :waste @03:37:04.04 LyS2.seek :full @03:37:04.18 LyS1.reconfigure DAmixLyS1 @03:37:04.28 LyS2.reconfigure DAmixLyS2 @03:37:04.85 LyS2.seek 1480ul @03:37:04.94 LyS1.seek 650ul @03:37:40.05 delay 5 seconds @03:37:45.08 Handoff.select :SPR @03:37:45.25 SPR.command "start 1 14" @03:37:46.23 Running Experiment-Rep #1 @03:37:46.24 delay 10 seconds @03:37:56.27 SPR.command "start 1 0" @03:38:00.11 Waiting up to 4:00 for SPR to enter state 1 @03:40:04.82 SPR.command "hold_on" @03:40:06.74 LyS2.seek 1130ul @03:40:06.83 LyS1.seek 300ul @03:40:55.85 delay 15 seconds @03:41:10.89 SPR.command "hold_off" @03:41:13.48 Waiting up to 25:00 for SPR to enter state 7 @04:01:17.90 Handoff.select :waste @04:01:18.07 LyS1.reconfigure LyS1config @04:01:18.15 LyS2.reconfigure LyS2config @04:01:18.25 Rep #1 SPR Analysis has Completed @04:01:18.83 Ended log /var/log/esp/spr18jul2503h.spr @04:01:18.85 SPR.command "stats_off" @04:01:19.36 Handoff.select :close @04:01:19.60
PV.seek :locked @04:01:20.63 ArS.seek :clear @04:01:20.83 LyS1.seek :clear @04:01:21.05 LyS2.seek :clear @04:01:21.26 AirS.seek :clear @04:01:38.45
ArS.coast @04:01:38.54 LyS1.coast @04:01:38.63 LyS2.coast @04:01:38.71 AirS.coast @04:01:38.81 TV.coast @04:01:38.89 SP.coast @04:01:38.98 SC.coast @04:01:39.07 PV.coast @04:01:39.16 PinPort.coast @04:01:39.41 Gate.power :motor,:OFF @04:01:39.42 Gate.power :heat,:OFF @04:01:39.46 Gate.power :main,:OFF @04:01:39.52 Gate.power :cartridge,:OFF @04:01:39.59 Gate.power :core,:OFF @04:01:39.62 Gate.power :analytic1,:OFF @04:01:39.67 Gate.power :analytic2,:OFF @04:01:39.73 Gate.power :analytic3,:OFF @04:01:39.76 Safely stopped and ready to power off @04:01:40.16 delay 30 seconds @04:02:10.29 Gate.power :main,:ON,limit to 5000mA @04:02:10.31 Gate.power :analytic1,:ON,limit to 5000mA @04:02:10.39 Gate.power :analytic2,:ON,limit to 5000mA @04:02:10.41 Gate.power :analytic3,:ON,limit to 5000mA @04:02:10.54 Powering core bus @04:02:12.90 SPR Module 1, FW Rev. B, FPGA Rev. C @04:02:13.10
ArS.configure ArSconfig @04:02:13.25 LyS1.configure LyS1config @04:02:13.42 LyS2.configure LyS2config @04:02:13.57 AirS.configure AirSconfig @04:02:13.75 TV.configure TVconfig @04:02:13.93 SP.configure SPconfig @04:02:14.11 SC.configure SCconfig @04:02:14.29 PV.configure PVconfig @04:02:14.47 PinPort.configure PPconfig @04:02:14.64 Sea.configure SPconfig @04:02:14.74 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @04:02:14.77 Gate.power :motor,:ON,limit to 5000mA @04:02:15.00 ArS.reconfigure ArSconfig @04:02:15.46 ArS.home.jog 0 @04:02:15.72 PV.reconfigure PVconfig @04:02:15.75 AirS.reconfigure AirSconfig @04:02:15.79 LyS2.reconfigure LyS2config @04:02:15.88 LyS1.reconfigure LyS1config @04:02:16.00 PinPort.reconfigure PPconfig @04:02:16.09 TV.reconfigure TVconfig @04:02:16.34 AirS.home.jog 0 @04:02:16.44 PV.home.jog 0 @04:02:16.49 LyS2.home.jog 0 @04:02:16.53 LyS1.home.jog 0 @04:02:16.59 PinPort.home.jog 0 @04:02:16.71 TV.home.jog 0 @04:02:17.17 ArS.reconfigure HomeMagSyringe @04:02:17.63 ArS.jog -278 @04:02:17.87 PinPort.reconfigure HomePP @04:02:17.93 PV.reconfigure HomePV @04:02:18.02 AirS.reconfigure HomeMagSyringe @04:02:18.06 LyS1.reconfigure HomeSyringe @04:02:18.14 LyS2.reconfigure HomeSyringe @04:02:18.32 TV.reconfigure HomeTV @04:02:18.47 PinPort.jog -2410 @04:02:18.63 PV.jog -1499 @04:02:18.66 AirS.jog -306 @04:02:18.70 LyS1.jog -274 @04:02:18.86 LyS2.jog -265 @04:02:18.91 TV.jog -542 @04:02:19.22 ArS.setRawPosition! -50 @04:02:19.32 ArS.seek :home @04:02:19.67 AirS.setRawPosition! -50 @04:02:19.76 AirS.seek :home @04:02:19.90 LyS2.setRawPosition! -50 @04:02:19.94 LyS1.setRawPosition! -50 @04:02:19.99 ArS.reconfigure ArSconfig @04:02:20.18 LyS2.seek :home @04:02:20.34 LyS1.seek :home @04:02:20.55 PV.setRawPosition! -50 @04:02:20.59 AirS.reconfigure AirSconfig @04:02:20.80 PV.seek :home @04:02:20.94 LyS1.reconfigure LyS1config @04:02:20.99 LyS2.reconfigure LyS2config @04:02:21.21 TV.setRawPosition! 0 @04:02:21.24 PV.reconfigure PVconfig @04:02:21.39 TV.seek :home @04:02:21.56 TV.reconfigure TVconfig @04:02:24.53 PinPort.setRawPosition! 0 @04:02:24.62 PinPort.seek :home @04:02:24.75 PinPort.reconfigure PPconfig @04:02:25.33
TV.seek :clear @04:02:26.99 TV.coast @04:02:27.09 SC.reconfigure HomeSC @04:02:27.18 SC.home.jog 0 @04:02:27.99 SC.reconfigure SCconfig @04:02:28.08 SC.seek 37 @04:02:28.18 Selecting Cartridge 34 @04:02:28.58 SC.seek 34 @04:02:29.56 LyS2.seek :locked @04:02:29.75 PV.seek :locked @04:02:29.96 AirS.seek :locked @04:02:34.07
Powering cartridge bus @04:02:36.21 Gate.setIR 1750,5000,0,6 @04:02:36.34 Gate.setIR 0,5000,0,6 @04:02:36.88 Loaded Lyse-n-Go Cartridge @04:02:36.89 PuckHeater.configure HeatConfig @04:02:36.99 Gate.power :heat,:ON,limit to 5000mA @04:02:37.31 Duration of filtering limited to 1:05:00 @04:02:37.74 Priming sample loop w/100ml, bypass w/10ml @04:02:37.90 SP.setPosition! 0ml @04:02:37.98 Intake.open @04:02:38.01 Exhaust.open @04:02:38.96 Sea @-42.9psia @04:02:38.99 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @04:02:44.75 Vacuum tare is 20% of full scale @04:02:44.76 PB.open @04:02:44.84 Exhaust.close @04:02:45.78 Sea @-42.9psia @04:02:45.92 SP.reconfigure SPprime @04:02:46.02 SP.seek 10ml,1:21 @04:02:56.94 SP.setPosition! 0ml @04:02:57.03 PB.close @04:02:57.07 Exhaust.open @04:02:57.97 Sea @-42.9psia @04:02:58.10 SP.reconfigure SPprime @04:02:58.20 SP.seek 100ml,8:51 @04:04:44.50 Pressurizing cartridge to 26psi @04:04:44.52 Intake.close @04:04:45.34 SP.setPosition! 0ml @04:04:45.41 PB.open @04:04:46.26 TV.seek :crack @04:04:47.11 TV.seek :clear @04:04:47.95 Intake.open @04:04:49.40 PB.close @04:04:50.20 Exhaust.close @04:04:51.02 Sea @-42.9psia @04:04:51.16 SP.reconfigure SPcharge @04:04:51.27 SP.seek 7.5ml,1:08 @04:05:04.37 Cartridge pressurized with 0.799ml to 26.3psi @04:05:04.67 delay 3 seconds @04:05:07.77 Cartridge at 21.3psi 3 seconds later @04:05:07.90 TV.seek :cartridge @04:05:09.75 TV.seek :clear @04:05:11.61 Sampling 500.0ml @04:05:11.63 SP.setPosition! 0ml @04:05:11.74 Exhaust.open @04:05:12.57 Sea @-42.9psia @04:05:12.72 SP.reconfigure SPsample @04:05:12.82 SP.seek 500ml,42:12 @04:05:13.73 Cartridge 34 sampling 0/500ml at 0.15ml/s with 2.8psi vacuum -39.0m deep (-42.9psia) @04:06:13.74 Cartridge 34 sampling 36/500ml at 0.59ml/s with 20.8psi vacuum -39.0m deep (-42.9psia) @04:07:13.73 Cartridge 34 sampling 71/500ml at 0.57ml/s with 24.5psi vacuum -39.0m deep (-42.9psia) @04:08:13.73 Cartridge 34 sampling 107/500ml at 0.60ml/s with 24.4psi vacuum -39.0m deep (-42.9psia) @04:09:13.74 Cartridge 34 sampling 142/500ml at 0.59ml/s with 23.0psi vacuum -39.0m deep (-42.9psia) @04:10:13.73 Cartridge 34 sampling 176/500ml at 0.58ml/s with 24.2psi vacuum -39.0m deep (-42.9psia) @04:11:13.74 Cartridge 34 sampling 211/500ml at 0.58ml/s with 23.4psi vacuum -39.0m deep (-42.9psia) @04:12:13.74 Cartridge 34 sampling 245/500ml at 0.57ml/s with 22.8psi vacuum -39.0m deep (-42.9psia) @04:13:13.73 Cartridge 34 sampling 280/500ml at 0.59ml/s with 24.6psi vacuum -39.0m deep (-42.9psia) @04:14:13.73 Cartridge 34 sampling 313/500ml at 0.57ml/s with 25.4psi vacuum -39.0m deep (-42.9psia) @04:15:13.73 Cartridge 34 sampling 347/500ml at 0.53ml/s with 19.7psi vacuum -39.0m deep (-42.9psia) @04:16:13.73 Cartridge 34 sampling 380/500ml at 0.57ml/s with 22.9psi vacuum -39.0m deep (-42.9psia) @04:17:13.73 Cartridge 34 sampling 413/500ml at 0.52ml/s with 19.3psi vacuum -39.0m deep (-42.9psia) @04:18:13.73 Cartridge 34 sampling 446/500ml at 0.54ml/s with 18.2psi vacuum -39.0m deep (-42.9psia) @04:19:13.73 Cartridge 34 sampling 478/500ml at 0.52ml/s with 21.5psi vacuum -39.0m deep (-42.9psia) @04:20:00.24 Intake.close @04:20:00.28 Exhaust.close @04:20:01.15 Sampled 500.0ml @04:20:01.29 TV.seek :bypass @04:20:02.95 TV.seek :clear @04:20:06.63 SPR.command "read_sequences" @04:20:07.25 SPR.command "stats_on" @04:20:07.33 SPR.command "start 2 0" @04:20:09.34 Begun log /var/log/esp/spr18jul2504h.spr @04:20:10.48 Gate.power :heat,:OFF @04:20:10.50 Gate.power :cartridge,:OFF @04:20:15.58 Powering cartridge bus @04:20:17.91 Gate.setIR 1750,5000,0,6 @04:20:18.04 Gate.setIR 0,5000,0,6 @04:20:18.15 Loaded Lyse-n-Go Cartridge @04:20:18.18 PuckHeater.configure HeatConfig @04:20:18.29 Gate.power :heat,:ON,limit to 5000mA @04:20:18.31 Handoff.select :waste @04:20:18.49 ArS.seek empty @04:20:36.27 PinPort.seek :cartridge @04:20:42.05 LyS1.seek 1350ul @04:20:48.03 PV.seek :reagent @04:20:48.92 AirS.seek :tVent @04:20:51.04 delay 2 seconds @04:20:53.07 AirS.reconfigure EvacAirS @04:20:53.24 AirS.seek :evac @04:21:15.58 delay 15 seconds @04:21:30.61 AirS.reconfigure AirSconfig @04:21:30.72 AirS.seek :tVent @04:21:44.97 delay 2 seconds @04:21:47.00 AirS.reconfigure EvacAirS @04:21:47.12 AirS.seek :evac @04:22:09.46 delay 15 seconds @04:22:24.49 AirS.reconfigure AirSconfig @04:22:24.59 AirS.seek :tVent @04:22:38.84 delay 2 seconds @04:22:40.87 AirS.reconfigure EvacAirS @04:22:40.98 AirS.seek :evac @04:23:03.31 delay 15 seconds @04:23:18.34 AirS.reconfigure AirSconfig @04:23:18.44 AirS.seek :tVent @04:23:32.69 delay 2 seconds @04:23:34.72 AirS.reconfigure EvacAirS @04:23:34.82 AirS.seek :evac @04:23:57.15 delay 15 seconds @04:24:12.18 delay 1:30 @04:25:42.20 AirS.reconfigure AirSconfig @04:25:42.30 AirS.seek :fVent @04:25:43.80 delay 15 seconds @04:25:58.83 LyS1.reconfigure SlowLyS1 @04:25:58.99 LyS1.seek 1050ul @04:26:20.09 delay 5 seconds @04:26:25.12 Handoff.select :blocked @04:26:25.36 LyS1.seek 450ul @04:27:07.42 delay 10 seconds @04:27:17.45 AirS.reconfigure SlowAirS @04:27:17.61 AirS.reconfigure to mix into 1.37806 parts LyS1 @04:27:18.14 AirS.seek evac @04:27:18.19 LyS1.seek 300ul @04:27:28.96 AirS.reconfigure AirSconfig @04:27:29.08 AirS.seek :full @04:27:43.33 LyS1.seek :empty @04:27:51.26 Slide::Error in PROCESSING -- IGNORED Lysis Syringe #1 positionErr at 190ul (actually 247ul) @04:27:51.30 LyS1.reconfigure SlowLyS1 @04:27:51.43 LyS1.home.jog 0 @04:27:51.59 PuckHeater.seek 84.2C @04:28:28.01 PuckHeater.hold 15:00,84.2C @04:43:30.74 LyS1.reconfigure LyS1config @04:43:30.84 LyS1.seek 900ul @04:43:37.08 AirS.seek :tVent @04:43:37.22 delay 2 seconds @04:43:39.25 AirS.reconfigure HalfSpeedAirS @04:43:39.35 AirS.seek :evac @04:44:07.13 delay 15 seconds @04:44:22.16 AirS.reconfigure AirSconfig @04:44:22.26 AirS.seek :tVent @04:44:36.51 delay 2 seconds @04:44:38.54 AirS.reconfigure HalfSpeedAirS @04:44:38.64 AirS.seek :evac @04:45:06.43 delay 15 seconds @04:45:21.46 AirS.reconfigure AirSconfig @04:45:21.56 AirS.seek :tVent @04:45:35.82 delay 2 seconds @04:45:37.85 AirS.reconfigure HalfSpeedAirS @04:45:37.98 AirS.seek :evac @04:46:05.82 delay 15 seconds @04:46:20.85 delay 1:00 @04:47:20.86 Handoff.select :waste @04:47:21.04 delay 15 seconds @04:47:36.07 AirS.seek :fVent @04:47:38.30 delay 10 seconds @04:47:48.33 AirS.reconfigure AirSconfig @04:47:48.43 AirS.seek :evac @04:47:49.93 Lysis Recovered in LyS1 @04:47:49.94 Lysis Complete @04:47:49.99 Handoff.select :waste @04:47:50.08 LyS2.seek :full @04:47:50.21 LyS1.reconfigure DAmixLyS1 @04:47:50.31 LyS2.reconfigure DAmixLyS2 @04:47:50.88 LyS2.seek 1480ul @04:47:50.92 LyS1.seek 650ul @04:48:26.09 delay 5 seconds @04:48:31.11 Handoff.select :SPR @04:48:31.29 SPR.command "start 1 14" @04:48:32.59 Running Experiment-Rep #1 @04:48:32.61 delay 10 seconds @04:48:42.66 SPR.command "start 1 0" @04:48:45.95 Waiting up to 4:00 for SPR to enter state 1 @04:50:50.24 SPR.command "hold_on" @04:50:52.16 LyS2.seek 1130ul @04:50:52.20 LyS1.seek 300ul @04:51:41.33 delay 15 seconds @04:51:56.35 SPR.command "hold_off" @04:51:58.90 Waiting up to 25:00 for SPR to enter state 7 @05:12:03.32 Handoff.select :waste @05:12:03.49 LyS1.reconfigure LyS1config @05:12:03.58 LyS2.reconfigure LyS2config @05:12:03.67 Rep #1 SPR Analysis has Completed @05:12:04.24 Ended log /var/log/esp/spr18jul2504h.spr @05:12:04.26 SPR.command "stats_off" @05:12:04.78 Handoff.select :close @05:12:05.03
PV.seek :locked @05:12:06.07 ArS.seek :clear @05:12:06.26 LyS1.seek :clear @05:12:06.48 LyS2.seek :clear @05:12:06.69 AirS.seek :clear @05:12:23.89
ArS.coast @05:12:23.97 LyS1.coast @05:12:24.05 LyS2.coast @05:12:24.14 AirS.coast @05:12:24.23 TV.coast @05:12:24.32 SP.coast @05:12:24.41 SC.coast @05:12:24.50 PV.coast @05:12:24.59 PinPort.coast @05:12:24.83 Gate.power :motor,:OFF @05:12:24.85 Gate.power :heat,:OFF @05:12:24.89 Gate.power :main,:OFF @05:12:24.95 Gate.power :cartridge,:OFF @05:12:25.00 Gate.power :core,:OFF @05:12:25.05 Gate.power :analytic1,:OFF @05:12:25.09 Gate.power :analytic2,:OFF @05:12:25.15 Gate.power :analytic3,:OFF @05:12:25.20 Safely stopped and ready to power off @05:12:25.40 delay 30 seconds @05:12:55.53 Gate.power :main,:ON,limit to 5000mA @05:12:55.56 Gate.power :analytic1,:ON,limit to 5000mA @05:12:55.63 Gate.power :analytic2,:ON,limit to 5000mA @05:12:55.69 Gate.power :analytic3,:ON,limit to 5000mA @05:12:55.79 Powering core bus @05:12:58.17 SPR Module 1, FW Rev. B, FPGA Rev. C @05:12:58.33
ArS.configure ArSconfig @05:12:58.48 LyS1.configure LyS1config @05:12:58.66 LyS2.configure LyS2config @05:12:58.82 AirS.configure AirSconfig @05:12:59.00 TV.configure TVconfig @05:12:59.17 SP.configure SPconfig @05:12:59.36 SC.configure SCconfig @05:12:59.54 PV.configure PVconfig @05:12:59.71 PinPort.configure PPconfig @05:12:59.89 Sea.configure SPconfig @05:12:59.99 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @05:13:00.01 Gate.power :motor,:ON,limit to 5000mA @05:13:00.27 ArS.reconfigure ArSconfig @05:13:00.68 ArS.home.jog 0 @05:13:00.94 PV.reconfigure PVconfig @05:13:00.97 TV.reconfigure TVconfig @05:13:01.01 AirS.reconfigure AirSconfig @05:13:01.11 LyS1.reconfigure LyS1config @05:13:01.24 PinPort.reconfigure PPconfig @05:13:01.32 LyS2.reconfigure LyS2config @05:13:01.62 TV.home.jog 0 @05:13:01.65 PV.home.jog 0 @05:13:01.71 AirS.home.jog 0 @05:13:01.75 LyS1.home.jog 0 @05:13:01.82 PinPort.home.jog 0 @05:13:01.87 LyS2.home.jog 0 @05:13:02.44 ArS.reconfigure HomeMagSyringe @05:13:02.77 ArS.jog -278 @05:13:03.00 LyS2.reconfigure HomeSyringe @05:13:03.07 LyS1.reconfigure HomeSyringe @05:13:03.17 PV.reconfigure HomePV @05:13:03.22 AirS.reconfigure HomeMagSyringe @05:13:03.28 TV.reconfigure HomeTV @05:13:03.37 PinPort.reconfigure HomePP @05:13:03.68 LyS2.jog -265 @05:13:03.73 LyS1.jog -269 @05:13:03.82 PV.jog -1499 @05:13:03.87 AirS.jog -309 @05:13:03.93 TV.jog -536 @05:13:03.99 PinPort.jog -2410 @05:13:04.34 ArS.setRawPosition! -50 @05:13:04.43 ArS.seek :home @05:13:04.62 LyS2.setRawPosition! -50 @05:13:04.74 LyS2.seek :home @05:13:04.89 LyS1.setRawPosition! -50 @05:13:04.93 ArS.reconfigure ArSconfig @05:13:04.98 AirS.setRawPosition! -50 @05:13:05.16 LyS1.seek :home @05:13:05.33 AirS.seek :home @05:13:05.52 PV.setRawPosition! -50 @05:13:05.54 LyS2.reconfigure LyS2config @05:13:05.73 PV.seek :home @05:13:05.94 LyS1.reconfigure LyS1config @05:13:05.96 AirS.reconfigure AirSconfig @05:13:06.22 TV.setRawPosition! 0 @05:13:06.25 PV.reconfigure PVconfig @05:13:06.42 TV.seek :home @05:13:06.56 TV.reconfigure TVconfig @05:13:09.97 PinPort.setRawPosition! 0 @05:13:10.07 PinPort.seek :home @05:13:10.19 PinPort.reconfigure PPconfig @05:13:10.78
TV.seek :clear @05:13:12.43 TV.coast @05:13:12.53 SC.reconfigure HomeSC @05:13:12.63 SC.home.jog 0 @05:13:13.45 SC.reconfigure SCconfig @05:13:13.55 SC.seek 34 @05:13:13.69 Selecting Cartridge 32 @05:13:14.11 SC.seek 32 @05:13:14.91 LyS2.seek :locked @05:13:15.10 PV.seek :locked @05:13:15.31 AirS.seek :locked @05:13:19.44
Powering cartridge bus @05:13:21.57 Gate.setIR 1750,5000,0,6 @05:13:21.70 Gate.setIR 0,5000,0,6 @05:13:21.80 Loaded Lyse-n-Go Cartridge @05:13:21.81 PuckHeater.configure HeatConfig @05:13:21.92 Gate.power :heat,:ON,limit to 5000mA @05:13:22.71 Duration of filtering limited to 1:05:00 @05:13:23.06 Priming sample loop w/100ml, bypass w/10ml @05:13:23.19 SP.setPosition! 0ml @05:13:23.28 Intake.open @05:13:23.33 Exhaust.open @05:13:24.24 Sea @-42.9psia @05:13:24.26 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @05:13:30.02 Vacuum tare is 20% of full scale @05:13:30.04 PB.open @05:13:30.10 Exhaust.close @05:13:31.05 Sea @-42.9psia @05:13:31.18 SP.reconfigure SPprime @05:13:31.29 SP.seek 10ml,1:21 @05:13:42.21 SP.setPosition! 0ml @05:13:42.31 PB.close @05:13:42.40 Exhaust.open @05:13:43.27 Sea @-42.9psia @05:13:43.41 SP.reconfigure SPprime @05:13:43.51 SP.seek 100ml,8:51 @05:15:29.54 Pressurizing cartridge to 26psi @05:15:29.55 Intake.close @05:15:30.33 SP.setPosition! 0ml @05:15:30.40 PB.open @05:15:31.26 TV.seek :crack @05:15:32.10 TV.seek :clear @05:15:32.96 Intake.open @05:15:34.37 PB.close @05:15:35.14 Exhaust.close @05:15:35.93 Sea @-42.9psia @05:15:36.07 SP.reconfigure SPcharge @05:15:36.17 SP.seek 7.5ml,1:08 @05:15:47.07 Cartridge pressurized with 0.549ml to 26.1psi @05:15:47.38 delay 3 seconds @05:15:50.48 Cartridge at 18.8psi 3 seconds later @05:15:50.61 TV.seek :cartridge @05:15:52.46 TV.seek :clear @05:15:54.32 Sampling 500.0ml @05:15:54.34 SP.setPosition! 0ml @05:15:54.46 Exhaust.open @05:15:55.28 Sea @-42.9psia @05:15:55.43 SP.reconfigure SPsample @05:15:55.53 SP.seek 500ml,42:12 @05:15:56.44 Cartridge 32 sampling 0/500ml at 0.14ml/s with 2.3psi vacuum -39.0m deep (-42.9psia) @05:16:56.44 Cartridge 32 sampling 35/500ml at 0.58ml/s with 20.2psi vacuum -39.0m deep (-42.9psia) @05:17:56.44 Cartridge 32 sampling 71/500ml at 0.59ml/s with 25.9psi vacuum -39.0m deep (-42.9psia) @05:18:56.45 Cartridge 32 sampling 106/500ml at 0.61ml/s with 24.0psi vacuum -39.0m deep (-42.9psia) @05:19:56.45 Cartridge 32 sampling 140/500ml at 0.58ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @05:20:56.45 Cartridge 32 sampling 175/500ml at 0.58ml/s with 24.9psi vacuum -39.0m deep (-42.9psia) @05:21:56.43 Cartridge 32 sampling 208/500ml at 0.54ml/s with 25.2psi vacuum -39.0m deep (-42.9psia) @05:22:56.45 Cartridge 32 sampling 242/500ml at 0.57ml/s with 23.7psi vacuum -39.0m deep (-42.9psia) @05:23:56.44 Cartridge 32 sampling 276/500ml at 0.55ml/s with 22.6psi vacuum -39.0m deep (-42.9psia) @05:24:56.45 Cartridge 32 sampling 309/500ml at 0.56ml/s with 26.5psi vacuum -39.0m deep (-42.9psia) @05:25:56.45 Cartridge 32 sampling 341/500ml at 0.54ml/s with 22.4psi vacuum -39.0m deep (-42.9psia) @05:26:56.44 Cartridge 32 sampling 374/500ml at 0.51ml/s with 20.4psi vacuum -39.0m deep (-42.9psia) @05:27:56.45 Cartridge 32 sampling 405/500ml at 0.53ml/s with 28.4psi vacuum -39.0m deep (-42.9psia) @05:28:56.44 Cartridge 32 sampling 437/500ml at 0.51ml/s with 21.6psi vacuum -39.0m deep (-42.9psia) @05:29:56.45 Cartridge 32 sampling 468/500ml at 0.48ml/s with 26.5psi vacuum -39.0m deep (-42.9psia) @05:30:56.45 Cartridge 32 sampling 498/500ml at 0.50ml/s with 28.5psi vacuum -39.0m deep (-42.9psia) @05:31:04.82 Intake.close @05:31:04.93 Exhaust.close @05:31:05.69 Sampled 500.0ml @05:31:05.83 TV.seek :bypass @05:31:07.49 TV.seek :clear @05:31:11.48 SPR.command "read_sequences" @05:31:11.55 SPR.command "stats_on" @05:31:11.62 SPR.command "start 2 0" @05:31:13.63 Begun log /var/log/esp/spr18jul2505h.spr @05:31:14.65 Gate.power :heat,:OFF @05:31:14.67 Gate.power :cartridge,:OFF @05:31:19.82 Powering cartridge bus @05:31:21.97 Gate.setIR 1750,5000,0,6 @05:31:22.10 Gate.setIR 0,5000,0,6 @05:31:22.20 Loaded Lyse-n-Go Cartridge @05:31:22.21 PuckHeater.configure HeatConfig @05:31:22.35 Gate.power :heat,:ON,limit to 5000mA @05:31:22.38 Handoff.select :waste @05:31:22.57 ArS.seek empty @05:31:40.35 PinPort.seek :cartridge @05:31:46.13 LyS1.seek 1350ul @05:31:52.13 PV.seek :reagent @05:31:53.01 AirS.seek :tVent @05:31:55.13 delay 2 seconds @05:31:57.16 AirS.reconfigure EvacAirS @05:31:57.27 AirS.seek :evac @05:32:19.63 delay 15 seconds @05:32:34.66 AirS.reconfigure AirSconfig @05:32:34.77 AirS.seek :tVent @05:32:49.03 delay 2 seconds @05:32:51.06 AirS.reconfigure EvacAirS @05:32:51.16 AirS.seek :evac @05:33:13.48 delay 15 seconds @05:33:28.51 AirS.reconfigure AirSconfig @05:33:28.62 AirS.seek :tVent @05:33:42.87 delay 2 seconds @05:33:44.90 AirS.reconfigure EvacAirS @05:33:45.01 AirS.seek :evac @05:34:07.33 delay 15 seconds @05:34:22.36 AirS.reconfigure AirSconfig @05:34:22.46 AirS.seek :tVent @05:34:36.71 delay 2 seconds @05:34:38.75 AirS.reconfigure EvacAirS @05:34:38.85 AirS.seek :evac @05:35:01.18 delay 15 seconds @05:35:16.21 delay 1:30 @05:36:46.24 AirS.reconfigure AirSconfig @05:36:46.35 AirS.seek :fVent @05:36:47.81 delay 15 seconds @05:37:02.85 LyS1.reconfigure SlowLyS1 @05:37:03.02 LyS1.seek 1050ul @05:37:24.11 delay 5 seconds @05:37:29.13 Handoff.select :blocked @05:37:29.38 LyS1.seek 450ul @05:38:11.47 delay 10 seconds @05:38:21.50 AirS.reconfigure SlowAirS @05:38:21.65 AirS.reconfigure to mix into 1.37806 parts LyS1 @05:38:22.17 AirS.seek evac @05:38:22.21 LyS1.seek 300ul @05:38:33.03 AirS.reconfigure AirSconfig @05:38:33.14 AirS.seek :full @05:38:47.39 LyS1.seek :empty @05:39:08.55 PuckHeater.seek 84.2C @05:39:38.63 PuckHeater.hold 15:00,84.2C @05:54:39.19 LyS1.reconfigure LyS1config @05:54:39.34 LyS1.seek 900ul @05:54:47.72 AirS.seek :tVent @05:54:47.89 delay 2 seconds @05:54:49.92 AirS.reconfigure HalfSpeedAirS @05:54:50.02 AirS.seek :evac @05:55:17.77 delay 15 seconds @05:55:32.80 AirS.reconfigure AirSconfig @05:55:32.90 AirS.seek :tVent @05:55:47.16 delay 2 seconds @05:55:49.19 AirS.reconfigure HalfSpeedAirS @05:55:49.31 AirS.seek :evac @05:56:17.10 delay 15 seconds @05:56:32.13 AirS.reconfigure AirSconfig @05:56:32.24 AirS.seek :tVent @05:56:46.49 delay 2 seconds @05:56:48.52 AirS.reconfigure HalfSpeedAirS @05:56:48.62 AirS.seek :evac @05:57:16.37 delay 15 seconds @05:57:31.40 delay 1:00 @05:58:31.42 Handoff.select :waste @05:58:31.68 delay 15 seconds @05:58:46.71 AirS.seek :fVent @05:58:49.00 delay 10 seconds @05:58:59.05 AirS.reconfigure AirSconfig @05:58:59.15 AirS.seek :evac @05:59:00.63 Lysis Recovered in LyS1 @05:59:00.65 Lysis Complete @05:59:00.67 Handoff.select :waste @05:59:00.79 LyS2.seek :full @05:59:00.91 LyS1.reconfigure DAmixLyS1 @05:59:01.01 LyS2.reconfigure DAmixLyS2 @05:59:01.57 LyS2.seek 1480ul @05:59:01.63 LyS1.seek 650ul @05:59:36.77 delay 5 seconds @05:59:41.79 Handoff.select :SPR @05:59:41.96 SPR.command "start 1 14" @05:59:43.38 Running Experiment-Rep #1 @05:59:43.40 delay 10 seconds @05:59:53.44 SPR.command "start 1 0" @05:59:56.74 Waiting up to 4:00 for SPR to enter state 1 @06:02:01.03 SPR.command "hold_on" @06:02:02.94 LyS2.seek 1130ul @06:02:03.06 LyS1.seek 300ul @06:02:52.07 delay 15 seconds @06:03:07.11 SPR.command "hold_off" @06:03:09.69 Waiting up to 25:00 for SPR to enter state 7 @06:23:14.11 Handoff.select :waste @06:23:14.29 LyS1.reconfigure LyS1config @06:23:14.38 LyS2.reconfigure LyS2config @06:23:14.48 Rep #1 SPR Analysis has Completed @06:23:15.03 Ended log /var/log/esp/spr18jul2505h.spr @06:23:15.05 SPR.command "stats_off" @06:23:15.56 Handoff.select :close @06:23:15.80
PV.seek :locked @06:23:16.83 ArS.seek :clear @06:23:17.04 LyS1.seek :clear @06:23:17.25 LyS2.seek :clear @06:23:17.46 AirS.seek :clear @06:23:34.59
ArS.coast @06:23:34.69 LyS1.coast @06:23:34.77 LyS2.coast @06:23:34.85 AirS.coast @06:23:34.95 TV.coast @06:23:35.04 SP.coast @06:23:35.13 SC.coast @06:23:35.21 PV.coast @06:23:35.31 PinPort.coast @06:23:35.55 Gate.power :motor,:OFF @06:23:35.56 Gate.power :heat,:OFF @06:23:35.62 Gate.power :main,:OFF @06:23:35.66 Gate.power :cartridge,:OFF @06:23:35.72 Gate.power :core,:OFF @06:23:35.76 Gate.power :analytic1,:OFF @06:23:35.81 Gate.power :analytic2,:OFF @06:23:35.86 Gate.power :analytic3,:OFF @06:23:35.91 Safely stopped and ready to power off @06:23:36.11 delay 30 seconds @06:24:06.24 Gate.power :main,:ON,limit to 5000mA @06:24:06.27 Gate.power :analytic1,:ON,limit to 5000mA @06:24:06.35 Gate.power :analytic2,:ON,limit to 5000mA @06:24:06.41 Gate.power :analytic3,:ON,limit to 5000mA @06:24:06.50 Powering core bus @06:24:08.87 SPR Module 1, FW Rev. B, FPGA Rev. C @06:24:09.06
ArS.configure ArSconfig @06:24:09.22 LyS1.configure LyS1config @06:24:09.39 LyS2.configure LyS2config @06:24:09.54 AirS.configure AirSconfig @06:24:09.72 TV.configure TVconfig @06:24:09.89 SP.configure SPconfig @06:24:10.06 SC.configure SCconfig @06:24:10.25 PV.configure PVconfig @06:24:10.42 PinPort.configure PPconfig @06:24:10.59 Sea.configure SPconfig @06:24:10.69 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @06:24:10.71 Gate.power :motor,:ON,limit to 5000mA @06:24:10.99 ArS.reconfigure ArSconfig @06:24:11.44 ArS.home.jog 0 @06:24:11.71 TV.reconfigure TVconfig @06:24:11.75 AirS.reconfigure AirSconfig @06:24:11.83 LyS1.reconfigure LyS1config @06:24:11.92 PV.reconfigure PVconfig @06:24:12.01 LyS2.reconfigure LyS2config @06:24:12.08 PinPort.reconfigure PPconfig @06:24:12.35 TV.home.jog 0 @06:24:12.46 AirS.home.jog 0 @06:24:12.49 LyS1.home.jog 0 @06:24:12.52 PV.home.jog 0 @06:24:12.63 LyS2.home.jog 0 @06:24:12.71 PinPort.home.jog 0 @06:24:13.38 ArS.reconfigure HomeMagSyringe @06:24:13.64 ArS.jog -277 @06:24:13.70 PinPort.reconfigure HomePP @06:24:13.77 TV.reconfigure HomeTV @06:24:13.84 LyS2.reconfigure HomeSyringe @06:24:13.92 LyS1.reconfigure HomeSyringe @06:24:13.99 PV.reconfigure HomePV @06:24:14.07 AirS.reconfigure HomeMagSyringe @06:24:14.39 PinPort.jog -2410 @06:24:14.42 TV.jog -537 @06:24:14.55 LyS2.jog -265 @06:24:14.67 LyS1.jog -274 @06:24:14.72 PV.jog -1501 @06:24:14.79 AirS.jog -307 @06:24:15.14 ArS.setRawPosition! -50 @06:24:15.22 ArS.seek :home @06:24:15.46 LyS2.setRawPosition! -50 @06:24:15.57 LyS2.seek :home @06:24:15.72 LyS1.setRawPosition! -50 @06:24:15.76 ArS.reconfigure ArSconfig @06:24:15.81 AirS.setRawPosition! -50 @06:24:16.01 LyS1.seek :home @06:24:16.08 AirS.seek :home @06:24:16.33 PV.setRawPosition! -50 @06:24:16.36 LyS2.reconfigure LyS2config @06:24:16.55 PV.seek :home @06:24:16.72 LyS1.reconfigure LyS1config @06:24:16.75 AirS.reconfigure AirSconfig @06:24:16.99 TV.setRawPosition! 0 @06:24:17.02 PV.reconfigure PVconfig @06:24:17.17 TV.seek :home @06:24:17.33 TV.reconfigure TVconfig @06:24:20.34 PinPort.setRawPosition! 0 @06:24:20.42 PinPort.seek :home @06:24:20.55 PinPort.reconfigure PPconfig @06:24:21.13
TV.seek :clear @06:24:22.78 TV.coast @06:24:22.88 SC.reconfigure HomeSC @06:24:22.97 SC.home.jog 0 @06:24:23.78 SC.reconfigure SCconfig @06:24:23.88 SC.seek 32 @06:24:24.02 Selecting Cartridge 29 @06:24:24.43 SC.seek 29 @06:24:25.43 LyS2.seek :locked @06:24:25.63 PV.seek :locked @06:24:25.85 AirS.seek :locked @06:24:29.98
Powering cartridge bus @06:24:32.11 Gate.setIR 1750,5000,0,6 @06:24:32.24 Gate.setIR 0,5000,0,6 @06:24:32.29 Loaded Archive Cartridge @06:24:32.34 Gate.power :cartridge,:OFF @06:24:33.15 Duration of filtering limited to 1:05:00 @06:24:33.52 Priming sample loop w/100ml, bypass w/10ml @06:24:33.66 SP.setPosition! 0ml @06:24:33.74 Intake.open @06:24:33.82 Exhaust.open @06:24:34.64 Sea @-42.9psia @06:24:34.67 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @06:24:40.47 Vacuum tare is 20% of full scale @06:24:40.48 PB.open @06:24:40.56 Exhaust.close @06:24:41.43 Sea @-42.9psia @06:24:41.57 SP.reconfigure SPprime @06:24:41.68 SP.seek 10ml,1:21 @06:24:52.60 SP.setPosition! 0ml @06:24:52.69 PB.close @06:24:52.73 Exhaust.open @06:24:53.63 Sea @-42.9psia @06:24:53.77 SP.reconfigure SPprime @06:24:53.88 SP.seek 100ml,8:51 @06:26:40.04 Pressurizing cartridge to 26psi @06:26:40.05 Intake.close @06:26:40.88 SP.setPosition! 0ml @06:26:40.96 PB.open @06:26:41.81 TV.seek :crack @06:26:42.66 TV.seek :clear @06:26:43.51 Intake.open @06:26:44.95 PB.close @06:26:45.72 Exhaust.close @06:26:46.53 Sea @-42.9psia @06:26:46.67 SP.reconfigure SPcharge @06:26:46.78 SP.seek 7.5ml,1:08 @06:26:53.98 Cartridge pressurized with 0.235ml to 26.2psi @06:26:54.29 delay 3 seconds @06:26:57.38 Cartridge at 18.2psi 3 seconds later @06:26:57.52 TV.seek :cartridge @06:26:59.38 TV.seek :clear @06:27:01.24 Sampling 500.0ml @06:27:01.26 SP.setPosition! 0ml @06:27:01.38 Exhaust.open @06:27:02.21 Sea @-42.9psia @06:27:02.36 SP.reconfigure SPsample @06:27:02.47 SP.seek 500ml,42:12 @06:27:03.38 Cartridge 29 sampling 0/500ml at 0.15ml/s with 3.7psi vacuum -39.0m deep (-42.9psia) @06:28:03.38 Cartridge 29 sampling 25/500ml at 0.44ml/s with 23.6psi vacuum -39.0m deep (-42.9psia) @06:29:03.38 Cartridge 29 sampling 48/500ml at 0.33ml/s with 26.6psi vacuum -39.0m deep (-42.9psia) @06:30:03.39 Cartridge 29 sampling 68/500ml at 0.31ml/s with 22.0psi vacuum -39.0m deep (-42.9psia) @06:31:03.38 Cartridge 29 sampling 87/500ml at 0.31ml/s with 25.8psi vacuum -39.0m deep (-42.9psia) @06:32:03.38 Cartridge 29 sampling 105/500ml at 0.30ml/s with 20.4psi vacuum -38.9m deep (-42.9psia) @06:33:03.39 Cartridge 29 sampling 121/500ml at 0.25ml/s with 23.3psi vacuum -39.0m deep (-42.9psia) @06:34:03.39 Cartridge 29 sampling 136/500ml at 0.23ml/s with 21.7psi vacuum -39.0m deep (-42.9psia) @06:35:03.39 Cartridge 29 sampling 150/500ml at 0.24ml/s with 24.3psi vacuum -39.0m deep (-42.9psia) @06:36:03.39 Cartridge 29 sampling 163/500ml at 0.21ml/s with 24.9psi vacuum -39.0m deep (-42.9psia) @06:37:03.39 Cartridge 29 sampling 176/500ml at 0.22ml/s with 21.2psi vacuum -39.0m deep (-42.9psia) @06:38:03.38 Cartridge 29 sampling 188/500ml at 0.20ml/s with 23.1psi vacuum -39.0m deep (-42.9psia) @06:39:03.38 Cartridge 29 sampling 200/500ml at 0.20ml/s with 25.5psi vacuum -39.0m deep (-42.9psia) @06:40:03.38 Cartridge 29 sampling 213/500ml at 0.20ml/s with 31.9psi vacuum -39.0m deep (-42.9psia) @06:41:03.39 Cartridge 29 sampling 225/500ml at 0.20ml/s with 31.8psi vacuum -39.0m deep (-42.9psia) @06:42:03.38 Cartridge 29 sampling 237/500ml at 0.20ml/s with 32.4psi vacuum -39.0m deep (-42.9psia) @06:42:52.09 Flow averaged 0.26ml/s over 15:42 @06:42:52.16 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @06:42:52.82 Relieved excess vacuum in 9.2 @06:42:52.87 SP.seek 500ml,21:37 @06:42:58.30 Flow estimate reduced to 0.21ml/s @06:42:58.34 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @06:42:59.03 Relieved excess vacuum in 3.0 @06:42:59.56 SP.seek 500ml,21:33 @06:43:01.83 Flow estimate reduced to 0.17ml/s @06:43:01.85 Intake.close @06:43:01.92 Exhaust.close @06:43:02.80 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:43:02.96 Sampled 248.0ml @06:43:03.09 TV.seek :bypass @06:43:04.75 TV.seek :clear @06:43:06.72 PV.seek :reagent @06:43:07.61 Sample loop closed. Ready to Deliver Reagents @06:43:07.63 ArS.seek :sealed @06:43:11.97 ArS.seek :evac @06:43:25.95 delay 20 seconds @06:43:45.99 ArS.seek :clear @06:44:03.75 RNAlater soak for 10:00 @06:54:03.78 ArS.seek :tVent @06:54:06.40 delay 1 second @06:54:07.43 ArS.seek :evac @06:54:23.10 delay 10 seconds @06:54:33.13 Sample preserved and evacuated @06:54:33.35
PV.seek :locked @06:54:34.38 ArS.seek :clear @06:54:34.65 LyS2.seek :clear @06:54:34.84 AirS.seek :clear @06:54:52.21
ArS.coast @06:54:52.31 LyS1.coast @06:54:52.39 LyS2.coast @06:54:52.47 AirS.coast @06:54:52.56 TV.coast @06:54:52.64 SP.coast @06:54:52.73 SC.coast @06:54:52.82 PV.coast @06:54:52.91 PinPort.coast @06:54:53.14 Gate.power :motor,:OFF @06:54:53.16 Gate.power :main,:OFF @06:54:53.21 Gate.power :core,:OFF @06:54:53.27 Gate.power :analytic1,:OFF @06:54:53.31 Gate.power :analytic2,:OFF @06:54:53.34 Gate.power :analytic3,:OFF @06:54:53.40 Safely stopped and ready to power off @06:54:53.63 delay 30 seconds @06:55:23.76 Gate.power :main,:ON,limit to 5000mA @06:55:23.78 Gate.power :analytic1,:ON,limit to 5000mA @06:55:23.86 Gate.power :analytic2,:ON,limit to 5000mA @06:55:23.91 Gate.power :analytic3,:ON,limit to 5000mA @06:55:24.01 Powering core bus @06:55:26.38 SPR Module 1, FW Rev. B, FPGA Rev. C @06:55:26.57
ArS.configure ArSconfig @06:55:26.73 LyS1.configure LyS1config @06:55:26.90 LyS2.configure LyS2config @06:55:27.06 AirS.configure AirSconfig @06:55:27.23 TV.configure TVconfig @06:55:27.41 SP.configure SPconfig @06:55:27.59 SC.configure SCconfig @06:55:27.77 PV.configure PVconfig @06:55:27.95 PinPort.configure PPconfig @06:55:28.12 Sea.configure SPconfig @06:55:28.22 All elf controllers running firmware version 4.46 @06:55:28.26 Gate.power :motor,:ON,limit to 5000mA @06:55:28.43 ArS.reconfigure ArSconfig @06:55:28.80 ArS.home.jog 0 @06:55:29.17 AirS.reconfigure AirSconfig @06:55:29.20 LyS1.reconfigure LyS1config @06:55:29.25 PinPort.reconfigure PPconfig @06:55:29.36 PV.reconfigure PVconfig @06:55:29.40 LyS2.reconfigure LyS2config @06:55:29.52 TV.reconfigure TVconfig @06:55:29.84 LyS1.home.jog 0 @06:55:29.89 PinPort.home.jog 0 @06:55:29.95 AirS.home.jog 0 @06:55:30.01 PV.home.jog 0 @06:55:30.07 TV.home.jog 0 @06:55:30.11 LyS2.home.jog 0 @06:55:30.68 ArS.reconfigure HomeMagSyringe @06:55:31.06 ArS.jog -276 @06:55:31.33 PinPort.reconfigure HomePP @06:55:31.38 TV.reconfigure HomeTV @06:55:31.43 LyS2.reconfigure HomeSyringe @06:55:31.54 PV.reconfigure HomePV @06:55:31.60 LyS1.reconfigure HomeSyringe @06:55:31.66 AirS.reconfigure HomeMagSyringe @06:55:31.99 PinPort.jog -108 @06:55:32.03 TV.jog -536 @06:55:32.07 LyS2.jog -265 @06:55:32.18 PV.jog -1502 @06:55:32.75 LyS1.jog -280 @06:55:32.85 AirS.jog -294 @06:55:33.16 ArS.setRawPosition! -50 @06:55:33.26 ArS.seek :home @06:55:33.39 PinPort.setRawPosition! 0 @06:55:33.48 PinPort.seek :home @06:55:33.69 AirS.setRawPosition! -50 @06:55:33.73 LyS2.setRawPosition! -50 @06:55:33.77 LyS1.setRawPosition! -50 @06:55:33.83 ArS.reconfigure ArSconfig @06:55:33.92 PinPort.reconfigure PPconfig @06:55:34.14 AirS.seek :home @06:55:34.27 LyS1.seek :home @06:55:34.37 LyS2.seek :home @06:55:34.70 PV.setRawPosition! -50 @06:55:34.82 PV.seek :home @06:55:35.03 AirS.reconfigure AirSconfig @06:55:35.07 LyS2.reconfigure LyS2config @06:55:35.14 TV.setRawPosition! 0 @06:55:35.24 LyS1.reconfigure LyS1config @06:55:35.51 TV.seek :home @06:55:35.67 PV.reconfigure PVconfig @06:55:35.70 TV.reconfigure TVconfig @06:55:36.00
TV.seek :clear @06:55:37.67 TV.coast @06:55:37.77 SC.reconfigure HomeSC @06:55:37.86 SC.home.jog 0 @06:55:38.68 SC.reconfigure SCconfig @06:55:38.78 SC.seek 29 @06:55:38.90 Selecting Cartridge 28 @06:55:39.32 SC.seek 28 @06:55:39.89 LyS2.seek :locked @06:55:40.09 PV.seek :locked @06:55:40.29 AirS.seek :locked @06:55:44.42
Powering cartridge bus @06:55:46.55 Gate.setIR 1750,5000,0,6 @06:55:46.68 Gate.setIR 0,5000,0,6 @06:55:46.78 Loaded Lyse-n-Go Cartridge @06:55:46.79 PuckHeater.configure HeatConfig @06:55:46.91 Gate.power :heat,:ON,limit to 5000mA @06:55:47.24 Duration of filtering limited to 1:05:00 @06:55:47.59 Priming sample loop w/100ml, bypass w/10ml @06:55:47.72 SP.setPosition! 0ml @06:55:47.80 Intake.open @06:55:47.85 Exhaust.open @06:55:48.76 Sea @-42.9psia @06:55:48.79 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @06:55:54.56 Vacuum tare is 20% of full scale @06:55:54.57 PB.open @06:55:54.65 Exhaust.close @06:55:55.56 Sea @-42.9psia @06:55:55.69 SP.reconfigure SPprime @06:55:55.81 SP.seek 10ml,1:21 @06:56:06.72 SP.setPosition! 0ml @06:56:06.79 PB.close @06:56:06.85 Exhaust.open @06:56:07.76 Sea @-42.9psia @06:56:07.89 SP.reconfigure SPprime @06:56:08.00 SP.seek 100ml,8:51 @06:57:54.25 Pressurizing cartridge to 26psi @06:57:54.26 Intake.close @06:57:55.10 SP.setPosition! 0ml @06:57:55.18 PB.open @06:57:56.06 TV.seek :crack @06:57:56.92 TV.seek :clear @06:57:57.78 Intake.open @06:57:59.25 PB.close @06:58:00.01 Exhaust.close @06:58:00.84 Sea @-42.9psia @06:58:01.42 SP.reconfigure SPcharge @06:58:01.54 SP.seek 7.5ml,1:08 @06:58:05.50 Cartridge pressurized with 0.069ml to 26.3psi @06:58:05.81 delay 3 seconds @06:58:08.89 Cartridge at 11.2psi 3 seconds later @06:58:09.03 TV.seek :cartridge @06:58:10.91 TV.seek :clear @06:58:12.77 Sampling 500.0ml @06:58:12.78 SP.setPosition! 0ml @06:58:12.88 Exhaust.open @06:58:13.72 Sea @-42.9psia @06:58:13.86 SP.reconfigure SPsample @06:58:13.98 SP.seek 500ml,42:12 @06:58:14.89 Cartridge 28 sampling 0/500ml at 0.15ml/s with 57.9psi vacuum -39.0m deep (-42.9psia) @06:58:15.55 Flow estimate reduced to 0.77ml/s @06:58:15.58 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @06:58:39.44 Relieved excess vacuum in 24.4 @06:58:39.49 SP.seek 500ml,42:11 @06:58:41.05 Flow estimate reduced to 0.61ml/s @06:58:41.09 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @06:59:13.40 Relieved excess vacuum in 33.7 @06:59:13.46 SP.seek 500ml,42:10 @06:59:14.88 Cartridge 28 sampling 1/500ml at 0.20ml/s with 76.4psi vacuum -39.0m deep (-42.9psia) @06:59:15.01 Flow estimate reduced to 0.49ml/s @06:59:15.04 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @06:59:29.25 Relieved excess vacuum in 14.2 @06:59:29.32 SP.seek 500ml,42:09 @06:59:30.81 Flow estimate reduced to 0.39ml/s @06:59:30.89 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @06:59:40.07 Relieved excess vacuum in 10.6 @06:59:40.12 SP.seek 500ml,42:08 @06:59:41.68 Flow estimate reduced to 0.31ml/s @06:59:41.75 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @07:00:14.93 Cartridge 28 sampling 1/500ml at 0.00ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @07:00:23.75 Relieved excess vacuum in 43.4 @07:00:23.81 SP.seek 500ml,42:07 @07:00:25.34 Flow estimate reduced to 0.25ml/s @07:00:25.39 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @07:00:48.53 Relieved excess vacuum in 24.5 @07:00:48.61 SP.seek 500ml,42:07 @07:00:50.18 Flow estimate reduced to 0.20ml/s @07:00:50.24 Waiting up to 2:14 for vacuum to reduce from 76.4 to 18.0psi @07:01:14.88 Cartridge 28 sampling 1/500ml at 0.00ml/s with 19.8psi vacuum -39.0m deep (-42.9psia) @07:01:18.25 Relieved excess vacuum in 29.5 @07:01:18.29 SP.seek 500ml,42:06 @07:01:19.88 Flow estimate reduced to 0.16ml/s @07:01:19.91 Intake.close @07:01:20.00 Exhaust.close @07:01:20.87 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @07:01:21.02 Sampled 1.4ml @07:01:21.16 TV.seek :bypass @07:01:22.82 TV.seek :clear @07:01:26.32 SPR.command "read_sequences" @07:01:26.63 SPR.command "stats_on" @07:01:26.71 SPR.command "start 2 0" @07:01:28.71 Begun log /var/log/esp/spr18jul2507h.spr AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @07:01:31.76 Gate.power :heat,:OFF @07:01:31.78 Gate.power :cartridge,:OFF @07:01:36.85 Powering cartridge bus @07:01:38.98 Gate.setIR 1750,5000,0,6 @07:01:39.11 Gate.setIR 0,5000,0,6 @07:01:39.20 Loaded Lyse-n-Go Cartridge @07:01:39.22 PuckHeater.configure HeatConfig @07:01:39.34 Gate.power :heat,:ON,limit to 5000mA @07:01:39.36 Handoff.select :waste @07:01:39.56 ArS.seek empty @07:01:57.34 PinPort.seek :cartridge @07:02:03.13 LyS1.seek 1350ul @07:02:09.14 PV.seek :reagent @07:02:10.04 AirS.seek :tVent @07:02:12.16 delay 2 seconds @07:02:14.19 AirS.reconfigure EvacAirS @07:02:14.31 AirS.seek :evac @07:02:36.68 delay 15 seconds @07:02:51.71 AirS.reconfigure AirSconfig @07:02:51.81 AirS.seek :tVent @07:03:06.10 delay 2 seconds @07:03:08.13 AirS.reconfigure EvacAirS @07:03:08.23 AirS.seek :evac @07:03:30.62 delay 15 seconds @07:03:45.65 AirS.reconfigure AirSconfig @07:03:45.75 AirS.seek :tVent @07:04:00.02 delay 2 seconds @07:04:02.05 AirS.reconfigure EvacAirS @07:04:02.15 AirS.seek :evac @07:04:24.55 delay 15 seconds @07:04:39.58 AirS.reconfigure AirSconfig @07:04:39.68 AirS.seek :tVent @07:04:53.96 delay 2 seconds @07:04:55.99 AirS.reconfigure EvacAirS @07:04:56.09 AirS.seek :evac @07:05:18.47 delay 15 seconds @07:05:33.51 delay 1:30 @07:07:03.54 AirS.reconfigure AirSconfig @07:07:03.65 AirS.seek :fVent @07:07:05.14 delay 15 seconds @07:07:20.17 LyS1.reconfigure SlowLyS1 @07:07:20.32 LyS1.seek 1050ul @07:07:41.45 delay 5 seconds @07:07:46.47 Handoff.select :blocked @07:07:46.70 LyS1.seek 450ul @07:08:28.83 delay 10 seconds @07:08:38.86 AirS.reconfigure SlowAirS @07:08:39.02 AirS.reconfigure to mix into 1.37806 parts LyS1 @07:08:39.64 AirS.seek evac @07:08:39.67 LyS1.seek 300ul @07:08:50.46 AirS.reconfigure AirSconfig @07:08:50.57 AirS.seek :full @07:09:04.83 LyS1.seek :empty @07:09:26.05 PuckHeater.seek 84.3C @07:09:56.14 PuckHeater.hold 15:00,84.3C @07:24:54.56 LyS1.reconfigure LyS1config @07:24:54.67 LyS1.seek 900ul @07:25:03.07 AirS.seek :tVent @07:25:03.22 delay 2 seconds @07:25:05.26 AirS.reconfigure HalfSpeedAirS @07:25:05.36 AirS.seek :evac @07:25:33.16 delay 15 seconds @07:25:48.19 AirS.reconfigure AirSconfig @07:25:48.32 AirS.seek :tVent @07:26:02.58 delay 2 seconds @07:26:04.62 AirS.reconfigure HalfSpeedAirS @07:26:04.72 AirS.seek :evac @07:26:32.52 delay 15 seconds @07:26:47.55 AirS.reconfigure AirSconfig @07:26:47.66 AirS.seek :tVent @07:27:01.89 delay 2 seconds @07:27:03.93 AirS.reconfigure HalfSpeedAirS @07:27:04.03 AirS.seek :evac @07:27:31.84 delay 15 seconds @07:27:46.87 delay 1:00 @07:28:46.88 Handoff.select :waste @07:28:47.18 delay 15 seconds @07:29:02.24 AirS.seek :fVent @07:29:04.51 delay 10 seconds @07:29:14.54 AirS.reconfigure AirSconfig @07:29:14.66 AirS.seek :evac @07:29:16.18 Lysis Recovered in LyS1 @07:29:16.19 Lysis Complete @07:29:16.21 Handoff.select :waste @07:29:16.31 LyS2.seek :full @07:29:16.44 LyS1.reconfigure DAmixLyS1 @07:29:16.56 LyS2.reconfigure DAmixLyS2 @07:29:17.10 LyS2.seek 1480ul @07:29:17.21 LyS1.seek 650ul @07:29:52.30 delay 5 seconds @07:29:57.32 Handoff.select :SPR @07:29:57.47 SPR.command "start 1 14" @07:29:58.59 Running Experiment-Rep #1 @07:29:58.60 delay 10 seconds @07:30:08.65 SPR.command "start 1 0" @07:30:11.95 Waiting up to 4:00 for SPR to enter state 1 @07:32:16.23 SPR.command "hold_on" @07:32:18.64 LyS2.seek 1130ul @07:32:18.76 LyS1.seek 300ul @07:33:07.78 delay 15 seconds @07:33:22.80 SPR.command "hold_off" @07:33:26.36 Waiting up to 25:00 for SPR to enter state 7 @07:53:30.26 Handoff.select :waste @07:53:30.43 LyS1.reconfigure LyS1config @07:53:30.52 LyS2.reconfigure LyS2config @07:53:30.63 Rep #1 SPR Analysis has Completed @07:53:31.18 Ended log /var/log/esp/spr18jul2507h.spr @07:53:31.20 SPR.command "stats_off" @07:53:31.73 Handoff.select :close @07:53:31.97
PV.seek :locked @07:53:33.01 ArS.seek :clear @07:53:33.22 LyS1.seek :clear @07:53:33.42 LyS2.seek :clear @07:53:33.63 AirS.seek :clear @07:53:50.83
ArS.coast @07:53:50.93 LyS1.coast @07:53:51.02 LyS2.coast @07:53:51.11 AirS.coast @07:53:51.20 TV.coast @07:53:51.31 SP.coast @07:53:51.40 SC.coast @07:53:51.50 PV.coast @07:53:51.59 PinPort.coast @07:53:51.85 Gate.power :motor,:OFF @07:53:51.88 Gate.power :heat,:OFF @07:53:51.93 Gate.power :main,:OFF @07:53:51.98 Gate.power :cartridge,:OFF @07:53:52.03 Gate.power :core,:OFF @07:53:52.10 Gate.power :analytic1,:OFF @07:53:52.15 Gate.power :analytic2,:OFF @07:53:52.17 Gate.power :analytic3,:OFF @07:53:52.21 Safely stopped and ready to power off @07:53:52.42 delay 30 seconds @07:54:22.54 Gate.power :main,:ON,limit to 5000mA @07:54:22.58 Gate.power :analytic1,:ON,limit to 5000mA @07:54:22.64 Gate.power :analytic2,:ON,limit to 5000mA @07:54:22.69 Gate.power :analytic3,:ON,limit to 5000mA @07:54:22.81 Powering core bus @07:54:25.18 SPR Module 1, FW Rev. B, FPGA Rev. C @07:54:25.38
ArS.configure ArSconfig @07:54:25.54 LyS1.configure LyS1config @07:54:25.71 LyS2.configure LyS2config @07:54:25.86 AirS.configure AirSconfig @07:54:26.04 TV.configure TVconfig @07:54:26.22 SP.configure SPconfig @07:54:26.40 SC.configure SCconfig @07:54:26.58 PV.configure PVconfig @07:54:26.75 PinPort.configure PPconfig @07:54:26.93 Sea.configure SPconfig @07:54:27.02 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @07:54:27.05 Gate.power :motor,:ON,limit to 5000mA @07:54:27.22 ArS.reconfigure ArSconfig @07:54:27.69 ArS.home.jog 0 @07:54:28.17 PV.reconfigure PVconfig @07:54:28.29 AirS.reconfigure AirSconfig @07:54:28.37 PinPort.reconfigure PPconfig @07:54:28.54 TV.reconfigure TVconfig @07:54:28.62 LyS2.reconfigure LyS2config @07:54:28.79 LyS1.reconfigure LyS1config @07:54:29.15 PV.home.jog 0 @07:54:29.19 PinPort.home.jog 0 @07:54:29.26 AirS.home.jog 0 @07:54:29.31 TV.home.jog 0 @07:54:29.38 LyS2.home.jog 0 @07:54:29.41 LyS1.home.jog 0 @07:54:30.00 ArS.reconfigure HomeMagSyringe @07:54:30.37 ArS.jog -277 @07:54:30.63 PV.reconfigure HomePV @07:54:30.69 TV.reconfigure HomeTV @07:54:30.74 LyS2.reconfigure HomeSyringe @07:54:30.84 PinPort.reconfigure HomePP @07:54:30.91 LyS1.reconfigure HomeSyringe @07:54:31.00 AirS.reconfigure HomeMagSyringe @07:54:31.32 PV.jog -1497 @07:54:31.36 LyS2.jog -265 @07:54:31.44 TV.jog -537 @07:54:31.51 PinPort.jog -2410 @07:54:31.56 LyS1.jog -275 @07:54:31.66 AirS.jog -309 @07:54:31.99 ArS.setRawPosition! -50 @07:54:32.08 ArS.seek :home @07:54:32.30 LyS2.setRawPosition! -50 @07:54:32.42 LyS2.seek :home @07:54:32.58 AirS.setRawPosition! -50 @07:54:32.63 LyS1.setRawPosition! -50 @07:54:32.67 ArS.reconfigure ArSconfig @07:54:33.06 AirS.seek :home @07:54:33.25 LyS1.seek :home @07:54:33.60 PV.setRawPosition! -50 @07:54:33.63 LyS2.reconfigure LyS2config @07:54:33.83 PV.seek :home @07:54:34.00 TV.setRawPosition! 0 @07:54:34.05 AirS.reconfigure AirSconfig @07:54:34.15 LyS1.reconfigure LyS1config @07:54:34.29 TV.seek :home @07:54:34.51 TV.reconfigure TVconfig @07:54:34.54 PV.reconfigure PVconfig @07:54:37.57 PinPort.setRawPosition! 0 @07:54:37.66 PinPort.seek :home @07:54:37.80 PinPort.reconfigure PPconfig @07:54:38.39
TV.seek :clear @07:54:40.03 TV.coast @07:54:40.12 SC.reconfigure HomeSC @07:54:40.21 SC.home.jog 0 @07:54:41.02 SC.reconfigure SCconfig @07:54:41.11 SC.seek 28 @07:54:41.23 Selecting Cartridge 23 @07:54:41.65 SC.seek 23 @07:54:43.02 LyS2.seek :locked @07:54:43.21 PV.seek :locked @07:54:43.42 AirS.seek :locked @07:54:47.55
Powering cartridge bus @07:54:49.68 Gate.setIR 1750,5000,0,6 @07:54:49.81 Gate.setIR 0,5000,0,6 @07:54:49.91 Loaded Lyse-n-Go Cartridge @07:54:49.92 PuckHeater.configure HeatConfig @07:54:50.48 Gate.power :heat,:ON,limit to 5000mA @07:54:50.79 Duration of filtering limited to 1:05:00 @07:54:51.16 Priming sample loop w/100ml, bypass w/10ml @07:54:51.30 SP.setPosition! 0ml @07:54:51.38 Intake.open @07:54:51.42 Exhaust.open @07:54:52.32 Sea @-42.9psia @07:54:52.34 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @07:54:58.10 Vacuum tare is 20% of full scale @07:54:58.13 PB.open @07:54:58.20 Exhaust.close @07:54:59.08 Sea @-42.9psia @07:54:59.24 SP.reconfigure SPprime @07:54:59.35 SP.seek 10ml,1:21 @07:55:10.27 SP.setPosition! 0ml @07:55:10.37 PB.close @07:55:10.41 Exhaust.open @07:55:11.35 Sea @-42.9psia @07:55:11.48 SP.reconfigure SPprime @07:55:11.59 SP.seek 100ml,8:51 @07:56:57.99 Pressurizing cartridge to 26psi @07:56:58.00 Intake.close @07:56:58.83 SP.setPosition! 0ml @07:56:58.92 PB.open @07:56:59.78 TV.seek :crack @07:57:00.61 TV.seek :clear @07:57:01.46 Intake.open @07:57:02.89 PB.close @07:57:03.69 Exhaust.close @07:57:04.53 Sea @-42.9psia @07:57:04.67 SP.reconfigure SPcharge @07:57:04.78 SP.seek 7.5ml,1:08 @07:57:13.04 Cartridge pressurized with 0.315ml to 26.6psi @07:57:13.36 delay 3 seconds @07:57:16.45 Cartridge at 21.6psi 3 seconds later @07:57:16.59 TV.seek :cartridge @07:57:18.44 TV.seek :clear @07:57:20.31 Sampling 500.0ml @07:57:20.32 SP.setPosition! 0ml @07:57:20.45 Exhaust.open @07:57:21.31 Sea @-42.9psia @07:57:21.45 SP.reconfigure SPsample @07:57:21.55 SP.seek 500ml,42:12 @07:57:22.47 Cartridge 23 sampling 0/500ml at 0.15ml/s with 3.2psi vacuum -39.0m deep (-42.9psia) @07:58:22.47 Cartridge 23 sampling 36/500ml at 0.61ml/s with 26.2psi vacuum -39.0m deep (-42.9psia) @07:59:22.48 Cartridge 23 sampling 72/500ml at 0.62ml/s with 23.4psi vacuum -39.0m deep (-42.9psia) @08:00:22.48 Cartridge 23 sampling 107/500ml at 0.57ml/s with 24.7psi vacuum -39.0m deep (-42.9psia) @08:01:22.47 Cartridge 23 sampling 143/500ml at 0.58ml/s with 27.5psi vacuum -39.0m deep (-42.9psia) @08:02:22.47 Cartridge 23 sampling 177/500ml at 0.57ml/s with 26.1psi vacuum -39.0m deep (-42.9psia) @08:03:22.48 Cartridge 23 sampling 212/500ml at 0.55ml/s with 19.3psi vacuum -39.0m deep (-42.9psia) @08:04:22.47 Cartridge 23 sampling 247/500ml at 0.59ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @08:05:22.48 Cartridge 23 sampling 281/500ml at 0.54ml/s with 24.0psi vacuum -39.0m deep (-42.9psia) @08:06:22.48 Cartridge 23 sampling 315/500ml at 0.57ml/s with 27.4psi vacuum -39.0m deep (-42.9psia) @08:07:22.48 Cartridge 23 sampling 349/500ml at 0.54ml/s with 26.8psi vacuum -39.0m deep (-42.9psia) @08:08:22.48 Cartridge 23 sampling 382/500ml at 0.56ml/s with 26.2psi vacuum -39.0m deep (-42.9psia) @08:09:22.48 Cartridge 23 sampling 415/500ml at 0.52ml/s with 21.3psi vacuum -39.0m deep (-42.9psia) @08:10:22.48 Cartridge 23 sampling 447/500ml at 0.51ml/s with 19.8psi vacuum -39.0m deep (-42.9psia) @08:11:22.48 Cartridge 23 sampling 478/500ml at 0.51ml/s with 21.5psi vacuum -39.0m deep (-42.9psia) @08:12:04.24 Intake.close @08:12:04.32 Exhaust.close @08:12:05.17 Sampled 500.0ml @08:12:05.31 TV.seek :bypass @08:12:06.98 TV.seek :clear @08:12:10.93 SPR.command "read_sequences" @08:12:11.02 SPR.command "stats_on" @08:12:11.09 SPR.command "start 2 0" @08:12:13.09 Begun log /var/log/esp/spr18jul2508h.spr @08:12:14.27 Gate.power :heat,:OFF @08:12:14.30 Gate.power :cartridge,:OFF @08:12:19.39 Powering cartridge bus @08:12:21.52 Gate.setIR 1750,5000,0,6 @08:12:21.65 Gate.setIR 0,5000,0,6 @08:12:21.75 Loaded Lyse-n-Go Cartridge @08:12:21.76 PuckHeater.configure HeatConfig @08:12:21.87 Gate.power :heat,:ON,limit to 5000mA @08:12:21.89 Handoff.select :waste @08:12:22.09 ArS.seek empty @08:12:39.88 PinPort.seek :cartridge @08:12:45.68 LyS1.seek 1350ul @08:12:51.67 PV.seek :reagent @08:12:52.56 AirS.seek :tVent @08:12:54.68 delay 2 seconds @08:12:56.71 AirS.reconfigure EvacAirS @08:12:56.82 AirS.seek :evac @08:13:19.16 delay 15 seconds @08:13:34.19 AirS.reconfigure AirSconfig @08:13:34.31 AirS.seek :tVent @08:13:48.57 delay 2 seconds @08:13:50.60 AirS.reconfigure EvacAirS @08:13:50.70 AirS.seek :evac @08:14:13.06 delay 15 seconds @08:14:28.09 AirS.reconfigure AirSconfig @08:14:28.20 AirS.seek :tVent @08:14:42.47 delay 2 seconds @08:14:44.50 AirS.reconfigure EvacAirS @08:14:44.60 AirS.seek :evac @08:15:06.96 delay 15 seconds @08:15:21.99 AirS.reconfigure AirSconfig @08:15:22.10 AirS.seek :tVent @08:15:36.37 delay 2 seconds @08:15:38.40 AirS.reconfigure EvacAirS @08:15:38.51 AirS.seek :evac @08:16:00.87 delay 15 seconds @08:16:15.90 delay 1:30 @08:16:20.12 -> abort! "leak in Pinport" @08:16:20.17 CmdServer::Client::Error in brent -- Aborting Mission: leak in Pinport @08:16:20.22 SystemExit in brent -- leak in Pinport @08:16:21.22 Ended log /var/log/esp/spr18jul2508h.spr @08:16:21.24
SPR.command "stats_off" @08:16:21.67 SPR.command "start 1 14" @08:16:21.81 Handoff.select :close @08:16:22.08 AnalyticModule::SPR::Error in PROCESSING -- not started @08:16:22.16 Thread::ChildDied in MAIN -- :PROCESSING @08:16:22.27 Session Ended @08:16:22.32 Session Ended @10:24:10.95PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @10:24:10.98 >BREAK< ESP3 Gateway v0.99 for cartridge bus @10:24:12.52 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @10:24:14.04 Polling Can every 5:00 @10:24:14.14 Gate.power :analytic1,:ON,limit to 5000mA @10:24:14.16 Gate.power :analytic2,:ON,limit to 5000mA @10:24:14.17 Gate.power :analytic3,:ON,limit to 5000mA @10:24:14.86 ArS.configure ArSconfig @10:24:14.97 LyS1.configure LyS1config @10:24:15.11 LyS2.configure LyS2config @10:24:15.22 AirS.configure AirSconfig @10:24:15.36 TV.configure TVconfig @10:24:15.50 SP.configure SPconfig @10:24:15.65 SPR Module 1, FW Rev. B, FPGA Rev. C @10:24:15.71
SC.reconfigure SCconfig @10:24:15.78 SC.hold @10:24:15.85 SC.configure SCconfig @10:24:15.98 PV.configure PVconfig @10:24:16.11 PinPort.configure PPconfig @10:24:16.25 Sea.configure SPconfig @10:24:16.31 All elf controllers running firmware version 4.46 @10:24:16.39 Intake.close @10:24:16.42 Exhaust.close @10:24:16.52 PB.close @10:24:16.73
Loading /home/esp/esp2/mission/processAll.rb mission @10:24:16.78 Processing all remaining dry cartridges in: {-500 => 23, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2, 8, 9, 13, 18], wet: 56} @10:24:16.79 Sampling maximum of 250.0ml per cartridge @10:24:16.82 Fixing samples for 10:00, evacuating once @10:24:16.83 delay 30 seconds @10:24:47.71 SC.seek 28 @10:24:47.78 Selecting Cartridge 18 @10:24:48.15 SC.seek 18 @10:24:50.53 LyS2.seek :locked @10:24:50.69 PV.seek :locked @10:24:50.84 AirS.seek :locked @10:24:54.92
Powering cartridge bus @10:24:57.06 Gate.setIR 1750,5000,0,6 @10:24:57.18 Gate.setIR 0,5000,0,6 @10:24:57.25 Loaded Lyse-n-Go Cartridge @10:24:57.27 PuckHeater.configure HeatConfig @10:24:57.31 Gate.power :heat,:ON,limit to 5000mA @10:24:57.59 Duration of filtering limited to 1:05:00 @10:24:57.91 Priming sample loop w/100ml, bypass w/10ml @10:24:58.00 SP.setPosition! 0ml @10:24:58.04 Intake.open @10:24:58.07 Exhaust.open @10:24:58.96 Sea @-42.9psia @10:24:58.98 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @10:25:04.73 Vacuum tare is 20% of full scale @10:25:04.75 PB.open @10:25:04.81 Exhaust.close @10:25:05.93 Sea @-42.9psia @10:25:06.02 SP.reconfigure SPprime @10:25:06.08 SP.seek 10ml,1:21 @10:25:16.96 SP.setPosition! 0ml @10:25:17.01 PB.close @10:25:17.04 Exhaust.open @10:25:17.91 Sea @-42.9psia @10:25:18.00 SP.reconfigure SPprime @10:25:18.07 SP.seek 100ml,8:51 @10:27:04.49 Pressurizing cartridge to 26psi @10:27:04.50 Intake.close @10:27:05.24 SP.setPosition! 0ml @10:27:05.28 PB.open @10:27:06.09 TV.seek :crack @10:27:06.89 TV.seek :clear @10:27:07.70 Intake.open @10:27:09.13 PB.close @10:27:09.86 Exhaust.close @10:27:10.66 Sea @-42.9psia @10:27:10.75 SP.reconfigure SPcharge @10:27:10.81 SP.seek 7.5ml,1:08 @10:27:25.00 Cartridge pressurized with 0.944ml to 26.2psi @10:27:25.28 delay 3 seconds @10:27:28.37 Cartridge at 22.1psi 3 seconds later @10:27:28.46 TV.seek :cartridge @10:27:30.28 TV.seek :clear @10:27:32.09 Sampling 250.0ml @10:27:32.11 SP.setPosition! 0ml @10:27:32.14 Exhaust.open @10:27:32.97 Sea @-42.9psia @10:27:33.07 SP.reconfigure SPsample @10:27:33.14 SP.seek 250ml,21:21 @10:27:34.12 Cartridge 18 sampling 0/250ml at 0.17ml/s with 3.7psi vacuum -39.0m deep (-42.9psia) @10:28:34.13 Cartridge 18 sampling 36/250ml at 0.58ml/s with 24.6psi vacuum -39.0m deep (-42.9psia) @10:29:34.13 Cartridge 18 sampling 71/250ml at 0.58ml/s with 25.8psi vacuum -39.0m deep (-42.9psia) @10:30:34.13 Cartridge 18 sampling 105/250ml at 0.57ml/s with 23.5psi vacuum -39.0m deep (-42.9psia) @10:31:34.13 Cartridge 18 sampling 139/250ml at 0.57ml/s with 23.5psi vacuum -39.0m deep (-42.9psia) @10:32:28.48 Session Begun @10:32:34.15 Cartridge 18 sampling 173/250ml at 0.58ml/s with 24.1psi vacuum -39.0m deep (-42.9psia) @10:33:34.15 Cartridge 18 sampling 206/250ml at 0.55ml/s with 26.3psi vacuum -39.0m deep (-42.9psia) @10:34:34.12 Cartridge 18 sampling 240/250ml at 0.56ml/s with 26.7psi vacuum -39.0m deep (-42.9psia) @10:34:52.71 Intake.close @10:34:52.75 Exhaust.close @10:34:53.60 Sampled 250.0ml @10:34:53.72 TV.seek :bypass @10:34:55.36 TV.seek :clear @10:34:58.84 SPR.command "read_sequences" @10:34:58.90 SPR.command "stats_on" @10:34:59.67 SPR.command "start 2 0" @10:35:01.67 Begun log /var/log/esp/spr18jul2510h.spr @10:35:02.90 Gate.power :heat,:OFF @10:35:02.92 Gate.power :cartridge,:OFF @10:35:07.99 Powering cartridge bus @10:35:10.12 Gate.setIR 1750,5000,0,6 @10:35:10.26 Gate.setIR 0,5000,0,6 @10:35:10.35 Loaded Lyse-n-Go Cartridge @10:35:10.71 PuckHeater.configure HeatConfig @10:35:10.82 Gate.power :heat,:ON,limit to 5000mA @10:35:10.84 Handoff.select :waste @10:35:11.00 ArS.seek empty @10:35:28.75 PinPort.seek :cartridge @10:35:34.49 LyS1.seek 1350ul @10:35:40.47 PV.seek :reagent @10:35:41.36 AirS.seek :tVent @10:35:43.45 delay 2 seconds @10:35:45.48 AirS.reconfigure EvacAirS @10:35:45.59 AirS.seek :evac @10:36:07.93 delay 15 seconds @10:36:22.96 AirS.reconfigure AirSconfig @10:36:23.06 AirS.seek :tVent @10:36:37.30 delay 2 seconds @10:36:39.33 AirS.reconfigure EvacAirS @10:36:39.41 AirS.seek :evac @10:37:01.73 delay 15 seconds @10:37:16.77 AirS.reconfigure AirSconfig @10:37:16.86 AirS.seek :tVent @10:37:31.11 delay 2 seconds @10:37:33.14 AirS.reconfigure EvacAirS @10:37:33.23 AirS.seek :evac @10:37:55.54 delay 15 seconds @10:38:10.57 AirS.reconfigure AirSconfig @10:38:10.65 AirS.seek :tVent @10:38:24.88 delay 2 seconds @10:38:26.91 AirS.reconfigure EvacAirS @10:38:26.99 AirS.seek :evac @10:38:49.31 delay 15 seconds @10:39:04.34 delay 1:30 @10:40:34.37 AirS.reconfigure AirSconfig @10:40:34.47 AirS.seek :fVent @10:40:35.95 delay 15 seconds @10:40:50.99 LyS1.reconfigure SlowLyS1 @10:40:51.16 LyS1.seek 1050ul @10:41:12.25 delay 5 seconds @10:41:17.27 Handoff.select :blocked @10:41:17.48 LyS1.seek 450ul @10:41:59.53 delay 10 seconds @10:42:09.56 AirS.reconfigure SlowAirS @10:42:09.75 AirS.reconfigure to mix into 1.37806 parts LyS1 @10:42:10.24 AirS.seek evac @10:42:10.28 LyS1.seek 300ul @10:42:21.05 AirS.reconfigure AirSconfig @10:42:21.15 AirS.seek :full @10:42:35.37 LyS1.seek :empty @10:42:56.46 Slide::Error in PROCESSING -- IGNORED Lysis Syringe #1 positionErr at 0ul (actually 58ul) @10:42:56.51 LyS1.reconfigure SlowLyS1 @10:42:56.60 LyS1.home.jog 0 @10:42:56.77 PuckHeater.seek 84.3C @10:44:02.54 PuckHeater.hold 15:00,84.3C @10:58:58.37 LyS1.reconfigure LyS1config @10:58:58.48 LyS1.seek 900ul @10:59:06.35 AirS.seek :tVent @10:59:06.48 delay 2 seconds @10:59:08.51 AirS.reconfigure HalfSpeedAirS @10:59:08.62 AirS.seek :evac @10:59:36.45 delay 15 seconds @10:59:51.48 AirS.reconfigure AirSconfig @10:59:51.58 AirS.seek :tVent @11:00:05.82 delay 2 seconds @11:00:07.85 AirS.reconfigure HalfSpeedAirS @11:00:07.94 AirS.seek :evac @11:00:35.71 delay 15 seconds @11:00:50.74 AirS.reconfigure AirSconfig @11:00:50.83 AirS.seek :tVent @11:01:05.08 delay 2 seconds @11:01:07.11 AirS.reconfigure HalfSpeedAirS @11:01:07.21 AirS.seek :evac @11:01:34.98 delay 15 seconds @11:01:50.01 delay 1:00 @11:02:50.03 Handoff.select :waste @11:02:50.18 delay 15 seconds @11:03:05.21 AirS.seek :fVent @11:03:07.44 delay 10 seconds @11:03:17.47 AirS.reconfigure AirSconfig @11:03:17.57 AirS.seek :evac @11:03:19.03 Lysis Recovered in LyS1 @11:03:19.04 Lysis Complete @11:03:19.08 Handoff.select :waste @11:03:19.14 LyS2.seek :full @11:03:19.26 LyS1.reconfigure DAmixLyS1 @11:03:19.35 LyS2.reconfigure DAmixLyS2 @11:03:19.91 LyS1.seek 650ul @11:03:19.95 LyS2.seek 1480ul @11:03:55.10 delay 5 seconds @11:04:00.13 Handoff.select :SPR @11:04:00.28 SPR.command "start 1 14" @11:04:00.94 Running Experiment-Rep #1 @11:04:00.96 delay 10 seconds @11:04:10.99 SPR.command "start 1 0" @11:04:14.29 Waiting up to 4:00 for SPR to enter state 1 @11:06:18.58 SPR.command "hold_on" @11:06:20.92 LyS1.seek 300ul @11:06:20.97 LyS2.seek 1130ul @11:07:10.02 delay 15 seconds @11:07:25.05 SPR.command "hold_off" @11:07:28.19 Waiting up to 25:00 for SPR to enter state 7 @11:27:31.66 Handoff.select :waste @11:27:31.79 LyS1.reconfigure LyS1config @11:27:31.87 LyS2.reconfigure LyS2config @11:27:31.95 Rep #1 SPR Analysis has Completed @11:27:32.59 Ended log /var/log/esp/spr18jul2510h.spr @11:27:32.61 SPR.command "stats_off" @11:27:33.47 Handoff.select :close @11:27:33.71
PV.seek :locked @11:27:34.74 ArS.seek :clear @11:27:34.96 LyS1.seek :clear @11:27:35.18 LyS2.seek :clear @11:27:35.40 AirS.seek :clear @11:27:52.55
ArS.coast @11:27:52.63 LyS1.coast @11:27:52.71 LyS2.coast @11:27:52.78 AirS.coast @11:27:52.86 TV.coast @11:27:52.94 SP.coast @11:27:53.01 SC.coast @11:27:53.10 PV.coast @11:27:53.17 PinPort.coast @11:27:53.43 Gate.power :motor,:OFF @11:27:53.44 Gate.power :heat,:OFF @11:27:53.48 Gate.power :main,:OFF @11:27:53.52 Gate.power :cartridge,:OFF @11:27:53.55 Gate.power :core,:OFF @11:27:53.58 Gate.power :analytic1,:OFF @11:27:53.62 Gate.power :analytic2,:OFF @11:27:53.67 Gate.power :analytic3,:OFF @11:27:53.70 Safely stopped and ready to power off @11:27:53.91 delay 30 seconds @11:28:24.05 Gate.power :main,:ON,limit to 5000mA @11:28:24.06 Gate.power :analytic1,:ON,limit to 5000mA @11:28:24.11 Gate.power :analytic2,:ON,limit to 5000mA @11:28:24.16 Gate.power :analytic3,:ON,limit to 5000mA @11:28:24.25 Powering core bus @11:28:26.62 SPR Module 1, FW Rev. B, FPGA Rev. C @11:28:26.84
ArS.configure ArSconfig @11:28:27.00 LyS1.configure LyS1config @11:28:27.16 LyS2.configure LyS2config @11:28:27.33 AirS.configure AirSconfig @11:28:27.51 TV.configure TVconfig @11:28:27.68 SP.configure SPconfig @11:28:27.85 SC.configure SCconfig @11:28:28.03 PV.configure PVconfig @11:28:28.21 PinPort.configure PPconfig @11:28:28.38 Sea.configure SPconfig @11:28:28.46 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @11:28:28.49 Gate.power :motor,:ON,limit to 5000mA @11:28:29.29 PinPort.reconfigure PPconfig @11:28:29.41 AirS.reconfigure AirSconfig @11:28:29.47 PV.reconfigure PVconfig @11:28:29.52 LyS2.reconfigure LyS2config @11:28:29.56 LyS1.reconfigure LyS1config @11:28:29.62 ArS.reconfigure ArSconfig @11:28:29.68 TV.reconfigure TVconfig @11:28:30.01 PinPort.home.jog 0 @11:28:30.05 AirS.home.jog 0 @11:28:30.10 PV.home.jog 0 @11:28:30.15 LyS2.home.jog 0 @11:28:30.18 LyS1.home.jog 0 @11:28:30.22 ArS.home.jog 0 @11:28:30.29 TV.home.jog 0 @11:28:31.39 TV.reconfigure HomeTV @11:28:31.51 PinPort.reconfigure HomePP @11:28:31.89 LyS2.reconfigure HomeSyringe @11:28:32.02 LyS1.reconfigure HomeSyringe @11:28:32.09 ArS.reconfigure HomeMagSyringe @11:28:32.13 AirS.reconfigure HomeMagSyringe @11:28:32.22 PV.reconfigure HomePV @11:28:32.50 TV.jog -549 @11:28:32.57 PinPort.jog -2410 @11:28:32.66 LyS2.jog -262 @11:28:32.73 LyS1.jog -293 @11:28:32.77 PV.jog -1502 @11:28:32.85 ArS.jog -279 @11:28:32.92 AirS.jog -310 @11:28:33.63 LyS2.setRawPosition! -50 @11:28:33.73 LyS2.seek :home @11:28:33.90 AirS.setRawPosition! -50 @11:28:33.92 LyS1.setRawPosition! -50 @11:28:33.96 ArS.setRawPosition! -50 @11:28:34.13 AirS.seek :home @11:28:34.18 LyS1.seek :home @11:28:34.26 ArS.seek :home @11:28:34.62 PV.setRawPosition! -50 @11:28:34.64 LyS2.reconfigure LyS2config @11:28:34.79 PV.seek :home @11:28:34.99 TV.setRawPosition! 0 @11:28:35.02 AirS.reconfigure AirSconfig @11:28:35.06 ArS.reconfigure ArSconfig @11:28:35.16 LyS1.reconfigure LyS1config @11:28:35.34 TV.seek :home @11:28:35.61 TV.reconfigure TVconfig @11:28:35.63 PV.reconfigure PVconfig @11:28:38.72 PinPort.setRawPosition! 0 @11:28:38.80 PinPort.seek :home @11:28:38.91 PinPort.reconfigure PPconfig @11:28:39.46
TV.seek :clear @11:28:41.10 TV.coast @11:28:41.19 SC.reconfigure HomeSC @11:28:41.28 SC.home.jog 0 @11:28:42.08 SC.reconfigure SCconfig @11:28:42.18 SC.seek 18 @11:28:42.32 Selecting Cartridge 13 @11:28:42.73 SC.seek 13 @11:28:42.97 Failed Loading Cartridge 13 because Storage Carousel positionErr at 18 - 97 counts (actually 18) @11:28:43.01 Slide::Error in MAIN -- Storage Carousel positionErr at 18 - 97 counts (actually 18) @11:28:43.05 Retry #1 of 2 @11:28:43.08 ArS.coast @11:28:43.19 LyS1.coast @11:28:43.27 LyS2.coast @11:28:43.35 AirS.coast @11:28:43.43 SC.coast @11:28:43.52 PV.coast @11:28:43.61 PinPort.coast @11:28:43.84 Gate.power :motor,:OFF @11:28:43.86 Gate.power :main,:OFF @11:28:43.90 Gate.power :core,:OFF @11:28:43.93 Gate.power :analytic1,:OFF @11:28:43.97 Gate.power :analytic2,:OFF @11:28:44.01 Gate.power :analytic3,:OFF @11:28:44.20 Gate.power :main,:ON,limit to 5000mA @11:28:44.22 Gate.power :analytic1,:ON,limit to 5000mA @11:28:44.27 Gate.power :analytic2,:ON,limit to 5000mA @11:28:44.30 Gate.power :analytic3,:ON,limit to 5000mA @11:28:44.97 Powering core bus @11:28:46.77 SPR Module 1, FW Rev. B, FPGA Rev. C @11:28:47.31
ArS.configure ArSconfig @11:28:47.45 LyS1.configure LyS1config @11:28:47.97 LyS2.configure LyS2config @11:28:48.11 AirS.configure AirSconfig @11:28:48.30 TV.configure TVconfig @11:28:48.48 SP.configure SPconfig @11:28:48.65 SC.configure SCconfig @11:28:48.82 PV.configure PVconfig @11:28:48.99 PinPort.configure PPconfig @11:28:49.16 Sea.configure SPconfig @11:28:49.25 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @11:28:49.27 Gate.power :motor,:ON,limit to 5000mA @11:28:49.96 AirS.reconfigure AirSconfig @11:28:50.06 PinPort.reconfigure PPconfig @11:28:50.12 PV.reconfigure PVconfig @11:28:50.18 TV.reconfigure TVconfig @11:28:50.23 LyS1.reconfigure LyS1config @11:28:50.33 LyS2.reconfigure LyS2config @11:28:50.39 ArS.reconfigure ArSconfig @11:28:50.67 AirS.home.jog 0 @11:28:50.76 PinPort.home.jog 0 @11:28:50.83 TV.home.jog 0 @11:28:50.91 LyS2.home.jog 0 @11:28:50.94 LyS1.home.jog 0 @11:28:51.00 ArS.home.jog 0 @11:28:51.05 PV.home.jog 0 @11:28:52.15 TV.reconfigure HomeTV @11:28:52.22 AirS.reconfigure HomeMagSyringe @11:28:52.28 LyS1.reconfigure HomeSyringe @11:28:52.35 ArS.reconfigure HomeMagSyringe @11:28:52.41 PV.reconfigure HomePV @11:28:52.48 LyS2.reconfigure HomeSyringe @11:28:52.54 PinPort.reconfigure HomePP @11:28:52.87 TV.jog -548 @11:28:52.90 LyS1.jog -270 @11:28:52.99 AirS.jog -306 @11:28:53.06 ArS.jog -273 @11:28:53.11 PV.jog -245 @11:28:53.18 PinPort.jog -109 @11:28:53.23 LyS2.jog -272 @11:28:53.87 PinPort.setRawPosition! 0 @11:28:53.95 PinPort.seek :home @11:28:54.17 PV.setRawPosition! -50 @11:28:54.22 AirS.setRawPosition! -50 @11:28:54.29 LyS2.setRawPosition! -50 @11:28:54.32 ArS.setRawPosition! -50 @11:28:54.36 LyS1.setRawPosition! -50 @11:28:54.40 PinPort.reconfigure PPconfig @11:28:54.70 AirS.seek :home @11:28:54.78 PV.seek :home @11:28:54.84 LyS2.seek :home @11:28:54.89 ArS.seek :home @11:28:54.94 LyS1.seek :home @11:28:55.48 TV.setRawPosition! 0 @11:28:55.57 AirS.reconfigure AirSconfig @11:28:56.02 TV.seek :home @11:28:56.28 TV.reconfigure TVconfig @11:28:56.34 ArS.reconfigure ArSconfig @11:28:56.39 LyS1.reconfigure LyS1config @11:28:56.45 PV.reconfigure PVconfig @11:28:56.51 LyS2.reconfigure LyS2config @11:28:57.34
TV.seek :clear @11:28:59.00 TV.coast @11:28:59.09 SC.reconfigure HomeSC @11:28:59.17 SC.home.jog 0 @11:28:59.98 SC.reconfigure SCconfig @11:29:00.08 SC.seek 18 @11:29:00.21 Selecting Cartridge 9 @11:29:00.64 SC.seek 9 @11:29:00.90 Failed Loading Cartridge 9 because Storage Carousel positionErr at 18 - 89 counts (actually 18) @11:29:00.93 Slide::Error in MAIN -- Storage Carousel positionErr at 18 - 89 counts (actually 18) @11:29:00.96 Retry #2 of 2 @11:29:00.99 ArS.coast @11:29:01.09 LyS1.coast @11:29:01.17 LyS2.coast @11:29:01.26 AirS.coast @11:29:01.34 SC.coast @11:29:01.41 PV.coast @11:29:01.52 PinPort.coast @11:29:01.76 Gate.power :motor,:OFF @11:29:01.78 Gate.power :main,:OFF @11:29:01.82 Gate.power :core,:OFF @11:29:01.86 Gate.power :analytic1,:OFF @11:29:01.90 Gate.power :analytic2,:OFF @11:29:01.94 Gate.power :analytic3,:OFF @11:29:02.13 Gate.power :main,:ON,limit to 5000mA @11:29:02.14 Gate.power :analytic1,:ON,limit to 5000mA @11:29:02.20 Gate.power :analytic2,:ON,limit to 5000mA @11:29:02.22 Gate.power :analytic3,:ON,limit to 5000mA @11:29:02.90 Powering core bus @11:29:04.70 SPR Module 1, FW Rev. B, FPGA Rev. C @11:29:05.21
ArS.configure ArSconfig @11:29:05.35 LyS1.configure LyS1config @11:29:05.53 LyS2.configure LyS2config @11:29:05.67 AirS.configure AirSconfig @11:29:05.84 TV.configure TVconfig @11:29:06.03 SP.configure SPconfig @11:29:06.19 SC.configure SCconfig @11:29:06.36 PV.configure PVconfig @11:29:06.54 PinPort.configure PPconfig @11:29:06.73 Sea.configure SPconfig @11:29:06.82 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @11:29:06.84 Gate.power :motor,:ON,limit to 5000mA @11:29:07.30 AirS.reconfigure AirSconfig @11:29:07.40 ArS.reconfigure ArSconfig @11:29:07.54 LyS1.reconfigure LyS1config @11:29:07.76 AirS.home.jog 0 @11:29:07.79 ArS.home.jog 0 @11:29:07.83 LyS1.home.jog 0 @11:29:08.27 PinPort.reconfigure PPconfig @11:29:08.32 PV.reconfigure PVconfig @11:29:08.45 TV.reconfigure TVconfig @11:29:08.50 LyS2.reconfigure LyS2config @11:29:09.12 PinPort.home.jog 0 @11:29:09.17 PV.home.jog 0 @11:29:09.21 LyS2.home.jog 0 @11:29:09.26 TV.home.jog 0 @11:29:09.77 AirS.reconfigure HomeMagSyringe @11:29:09.91 LyS1.reconfigure HomeSyringe @11:29:09.99 ArS.reconfigure HomeMagSyringe @11:29:10.23 AirS.jog -305 @11:29:10.30 LyS1.jog -274 @11:29:10.35 ArS.jog -273 @11:29:10.68 TV.reconfigure HomeTV @11:29:10.76 PV.reconfigure HomePV @11:29:10.82 LyS2.reconfigure HomeSyringe @11:29:10.87 PinPort.reconfigure HomePP @11:29:11.14 TV.jog -545 @11:29:11.18 LyS2.jog -272 @11:29:11.36 PV.jog -249 @11:29:11.41 PinPort.jog -109 @11:29:11.66 AirS.setRawPosition! -50 @11:29:11.69 LyS1.setRawPosition! -50 @11:29:11.74 ArS.setRawPosition! -50 @11:29:11.90 AirS.seek :home @11:29:11.95 LyS1.seek :home @11:29:12.03 ArS.seek :home @11:29:12.36 PV.setRawPosition! -50 @11:29:12.39 LyS2.setRawPosition! -50 @11:29:12.45 PinPort.setRawPosition! 0 @11:29:12.64 PV.seek :home @11:29:12.73 PinPort.seek :home @11:29:12.80 LyS2.seek :home @11:29:13.12 AirS.reconfigure AirSconfig @11:29:13.20 LyS1.reconfigure LyS1config @11:29:13.26 PinPort.reconfigure PPconfig @11:29:13.33 ArS.reconfigure ArSconfig @11:29:13.61 TV.setRawPosition! 0 @11:29:13.65 LyS2.reconfigure LyS2config @11:29:13.68 PV.reconfigure PVconfig @11:29:13.92 TV.seek :home @11:29:14.08 TV.reconfigure TVconfig @11:29:14.42
TV.seek :clear @11:29:16.07 TV.coast @11:29:16.16 SC.reconfigure HomeSC @11:29:16.24 SC.home.jog 0 @11:29:17.05 SC.reconfigure SCconfig @11:29:17.13 SC.seek 18 @11:29:17.27 Selecting Cartridge 8 @11:29:17.69 SC.seek 8 @11:29:17.95 Failed Loading Cartridge 8 because Storage Carousel positionErr at 18 - 93 counts (actually 18) @11:29:18.01 Slide::Error in MAIN -- Storage Carousel positionErr at 18 - 93 counts (actually 18) @11:29:18.11 Thread::ChildDied in MAIN_scheduler -- :MAIN @11:29:18.22 Session Ended @11:59:08.99PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @11:59:09.01 >BREAK< ESP3 Gateway v0.99 for cartridge bus @11:59:10.62 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @11:59:12.19 Polling Can every 5:00 @11:59:12.31 Gate.power :analytic1,:ON,limit to 5000mA @11:59:12.33 Gate.power :analytic2,:ON,limit to 5000mA @11:59:12.34 Gate.power :analytic3,:ON,limit to 5000mA @11:59:13.05 ArS.configure ArSconfig @11:59:13.11 SPR Module 1, FW Rev. B, FPGA Rev. C @11:59:13.20
LyS1.configure LyS1config @11:59:13.37 LyS2.configure LyS2config @11:59:13.48 AirS.configure AirSconfig @11:59:13.61 TV.configure TVconfig @11:59:13.75 SP.configure SPconfig @11:59:13.90 SC.reconfigure SCconfig @11:59:13.96 SC.hold @11:59:14.02 SC.configure SCconfig @11:59:14.16 PV.configure PVconfig @11:59:14.31 PinPort.configure PPconfig @11:59:14.45 Sea.configure SPconfig @11:59:14.51 All elf controllers running firmware version 4.46 @11:59:14.58 Intake.close @11:59:14.63 Exhaust.close @11:59:14.68 PB.close @11:59:14.93
Loading /home/esp/esp2/mission/processAll.rb mission @11:59:15.01 Processing all remaining dry cartridges in: {-500 => 23, 1 => :spares, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 250 => 18, 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2], failed: [8, 9, 13], wet: 56} @11:59:15.02 Sampling maximum of 250.0ml per cartridge @11:59:15.03 Fixing samples for 10:00, evacuating once @11:59:15.04 delay 30 seconds @11:59:45.76 SC.seek 1 @11:59:45.84 Selecting Cartridge 2 @11:59:46.20 SC.seek 2 @11:59:46.78 LyS2.seek :locked @11:59:46.94 PV.seek :locked @11:59:47.09 AirS.seek :locked @11:59:51.18
Powering cartridge bus @11:59:53.31 Gate.setIR 1750,5000,0,6 @11:59:53.43 Gate.setIR 0,5000,0,6 @11:59:53.47 Loaded Archive Cartridge @11:59:53.48 Gate.power :cartridge,:OFF @11:59:53.78 Duration of filtering limited to 1:05:00 @11:59:54.08 Priming sample loop w/100ml, bypass w/10ml @11:59:54.16 SP.setPosition! 0ml @11:59:54.20 Intake.open @11:59:54.24 Exhaust.open @11:59:55.14 Sea @-42.9psia @11:59:55.16 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:00:00.92 Vacuum tare is 20% of full scale @12:00:00.94 PB.open @12:00:00.97 Exhaust.close @12:00:02.17 Sea @-42.9psia @12:00:02.27 SP.reconfigure SPprime @12:00:02.33 SP.seek 10ml,1:21 @12:00:13.20 SP.setPosition! 0ml @12:00:13.25 PB.close @12:00:13.32 Exhaust.open @12:00:14.11 Sea @-42.9psia @12:00:14.20 SP.reconfigure SPprime @12:00:14.28 SP.seek 100ml,8:51 @12:00:18.04 Session Begun @12:00:28.14 -> abort! "wrong cartridge" @12:00:28.17 CmdServer::Client::Error in preston -- Aborting Mission: wrong cartridge @12:00:28.22 SystemExit in preston -- wrong cartridge @12:00:28.29
SP.stop @12:00:28.63 Intake.close @12:00:28.81 Exhaust.close @12:00:29.72 Session Ended @12:02:38.68PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @12:02:38.74 >BREAK< ESP3 Gateway v0.99 for cartridge bus @12:02:40.56 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @12:02:42.69 Polling Can every 5:00 @12:02:42.80 Gate.power :analytic1,:ON,limit to 5000mA @12:02:42.81 Gate.power :analytic2,:ON,limit to 5000mA @12:02:42.82 Gate.power :analytic3,:ON,limit to 5000mA @12:02:43.51 ArS.configure ArSconfig @12:02:43.62 LyS1.configure LyS1config @12:02:43.76 LyS2.configure LyS2config @12:02:43.88 AirS.configure AirSconfig @12:02:44.04 SPR Module 1, FW Rev. B, FPGA Rev. C @12:02:44.10
TV.configure TVconfig @12:02:44.24 SP.configure SPconfig @12:02:44.38 SC.reconfigure SCconfig @12:02:44.43 SC.hold @12:02:44.49 SC.configure SCconfig @12:02:44.63 PV.configure PVconfig @12:02:44.76 PinPort.configure PPconfig @12:02:44.89 Sea.configure SPconfig @12:02:44.95 All elf controllers running firmware version 4.46 @12:02:45.03 Intake.close @12:02:45.08 Exhaust.close @12:02:45.12 PB.close @12:02:45.35
Loading /home/esp/esp2/mission/processAll.rb mission @12:02:45.41 Processing all remaining dry cartridges in: {-500 => 23, 1 => :spares, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 250 => 18, 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2, 8, 9, 13], wet: 56} @12:02:45.43 Sampling maximum of 1000.0ml per cartridge @12:02:45.44 Fixing samples for 10:00, evacuating once @12:02:45.46 delay 30 seconds @12:02:46.78 Session Begun @12:02:56.14 -> abort! "wrong volume" @12:02:56.17 CmdServer::Client::Error in preston -- Aborting Mission: wrong volume @12:02:56.22 SystemExit in preston -- wrong volume @12:02:56.40 ThreadError in preston -- killed thread @12:02:56.49 Session Ended @12:03:15.94PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @12:03:15.97 >BREAK< ESP3 Gateway v0.99 for cartridge bus @12:03:17.54 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @12:03:19.11 Polling Can every 5:00 @12:03:19.21 Gate.power :analytic1,:ON,limit to 5000mA @12:03:19.23 Gate.power :analytic2,:ON,limit to 5000mA @12:03:19.25 Gate.power :analytic3,:ON,limit to 5000mA @12:03:19.95 ArS.configure ArSconfig @12:03:20.02 SPR Module 1, FW Rev. B, FPGA Rev. C @12:03:20.12
LyS1.configure LyS1config @12:03:20.26 LyS2.configure LyS2config @12:03:20.37 AirS.configure AirSconfig @12:03:20.51 TV.configure TVconfig @12:03:20.66 SP.configure SPconfig @12:03:20.79 SC.reconfigure SCconfig @12:03:20.85 SC.hold @12:03:20.90 SC.configure SCconfig @12:03:21.03 PV.configure PVconfig @12:03:21.17 PinPort.configure PPconfig @12:03:21.31 Sea.configure SPconfig @12:03:21.36 All elf controllers running firmware version 4.46 @12:03:21.45 Intake.close @12:03:21.50 Exhaust.close @12:03:21.54 PB.close @12:03:21.79
Loading /home/esp/esp2/mission/processAll.rb mission @12:03:21.85 Processing all remaining dry cartridges in: {-500 => 23, 1 => :spares, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 250 => 18, 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2, 8, 9, 13], wet: 56} @12:03:21.86 Sampling maximum of 250.0ml per cartridge @12:03:21.87 Fixing samples for 10:00, evacuating once @12:03:21.89 delay 30 seconds @12:03:52.65 SC.seek 2 @12:03:52.73 Selecting Cartridge 13 @12:03:53.11 SC.seek 13 @12:03:55.69 LyS2.seek :locked @12:03:55.85 PV.seek :locked @12:03:56.01 AirS.seek :locked @12:04:00.10
Powering cartridge bus @12:04:02.24 Gate.setIR 1750,5000,0,6 @12:04:02.36 Gate.setIR 0,5000,0,6 @12:04:02.42 Loaded Lyse-n-Go Cartridge @12:04:02.43 PuckHeater.configure HeatConfig @12:04:02.48 Gate.power :heat,:ON,limit to 5000mA @12:04:02.77 Duration of filtering limited to 1:05:00 @12:04:03.10 Priming sample loop w/100ml, bypass w/10ml @12:04:03.19 SP.setPosition! 0ml @12:04:03.24 Intake.open @12:04:03.27 Exhaust.open @12:04:04.14 Sea @-42.9psia @12:04:04.16 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:04:09.88 Vacuum tare is 20% of full scale @12:04:09.90 PB.open @12:04:09.93 Exhaust.close @12:04:11.10 Sea @-42.9psia @12:04:11.20 SP.reconfigure SPprime @12:04:11.28 SP.seek 10ml,1:21 @12:04:22.14 SP.setPosition! 0ml @12:04:22.19 PB.close @12:04:22.25 Exhaust.open @12:04:23.10 Sea @-42.9psia @12:04:23.20 SP.reconfigure SPprime @12:04:23.28 SP.seek 100ml,8:51 @12:06:09.36 Pressurizing cartridge to 26psi @12:06:09.37 Intake.close @12:06:10.13 SP.setPosition! 0ml @12:06:10.17 PB.open @12:06:10.95 TV.seek :crack @12:06:11.76 TV.seek :clear @12:06:12.56 Intake.open @12:06:14.01 PB.close @12:06:14.72 Exhaust.close @12:06:15.51 Sea @-42.9psia @12:06:15.60 SP.reconfigure SPcharge @12:06:15.66 SP.seek 7.5ml,1:08 @12:06:22.99 Cartridge pressurized with 0.248ml to 26.4psi @12:06:23.26 delay 3 seconds @12:06:26.34 Cartridge at 19.8psi 3 seconds later @12:06:26.44 TV.seek :cartridge @12:06:28.28 TV.seek :clear @12:06:30.09 Sampling 250.0ml @12:06:30.10 SP.setPosition! 0ml @12:06:30.14 Exhaust.open @12:06:30.92 Sea @-42.9psia @12:06:31.03 SP.reconfigure SPsample @12:06:31.09 SP.seek 250ml,21:21 @12:06:32.09 Cartridge 13 sampling 0/250ml at 0.17ml/s with 3.0psi vacuum -39.0m deep (-42.9psia) @12:07:32.09 Cartridge 13 sampling 35/250ml at 0.60ml/s with 26.0psi vacuum -39.0m deep (-42.9psia) @12:08:32.08 Cartridge 13 sampling 70/250ml at 0.59ml/s with 18.6psi vacuum -39.0m deep (-42.9psia) @12:09:32.09 Cartridge 13 sampling 105/250ml at 0.60ml/s with 24.1psi vacuum -39.0m deep (-42.9psia) @12:10:32.10 Cartridge 13 sampling 139/250ml at 0.58ml/s with 19.2psi vacuum -39.0m deep (-42.9psia) @12:11:32.09 Cartridge 13 sampling 172/250ml at 0.54ml/s with 16.5psi vacuum -39.0m deep (-42.9psia) @12:12:32.09 Cartridge 13 sampling 206/250ml at 0.53ml/s with 24.4psi vacuum -39.0m deep (-42.9psia) @12:13:32.09 Cartridge 13 sampling 239/250ml at 0.56ml/s with 19.4psi vacuum -39.0m deep (-42.9psia) @12:13:51.79 Intake.close @12:13:51.83 Exhaust.close @12:13:52.64 Sampled 250.0ml @12:13:52.73 TV.seek :bypass @12:13:54.35 TV.seek :clear @12:13:57.81 SPR.command "read_sequences" @12:13:58.30 SPR.command "stats_on" @12:13:58.34 SPR.command "start 2 0" @12:14:00.34 Begun log /var/log/esp/spr18jul2512h.spr @12:14:01.15 Handoff.select :waste @12:14:01.28 ArS.seek empty @12:14:19.01 PinPort.seek :cartridge @12:14:24.72 LyS1.seek 1350ul @12:14:30.66 PV.seek :reagent @12:14:31.52 AirS.seek :tVent @12:14:33.59 delay 2 seconds @12:14:35.62 AirS.reconfigure EvacAirS @12:14:35.69 AirS.seek :evac @12:14:58.00 delay 15 seconds @12:15:13.03 AirS.reconfigure AirSconfig @12:15:13.10 AirS.seek :tVent @12:15:27.32 delay 2 seconds @12:15:29.35 AirS.reconfigure EvacAirS @12:15:29.40 AirS.seek :evac @12:15:51.71 delay 15 seconds @12:16:06.74 AirS.reconfigure AirSconfig @12:16:06.80 AirS.seek :tVent @12:16:21.01 delay 2 seconds @12:16:23.04 AirS.reconfigure EvacAirS @12:16:23.09 AirS.seek :evac @12:16:45.40 delay 15 seconds @12:17:00.43 AirS.reconfigure AirSconfig @12:17:00.49 AirS.seek :tVent @12:17:14.70 delay 2 seconds @12:17:16.73 AirS.reconfigure EvacAirS @12:17:16.79 AirS.seek :evac @12:17:39.08 delay 15 seconds @12:17:54.11 delay 1:30 @12:19:24.16 AirS.reconfigure AirSconfig @12:19:24.24 AirS.seek :fVent @12:19:25.70 delay 15 seconds @12:19:40.73 LyS1.reconfigure SlowLyS1 @12:19:40.85 LyS1.seek 1050ul @12:20:01.93 delay 5 seconds @12:20:06.95 Handoff.select :blocked @12:20:07.14 LyS1.seek 450ul @12:20:49.22 delay 10 seconds @12:20:59.26 AirS.reconfigure SlowAirS @12:20:59.38 AirS.reconfigure to mix into 1.37806 parts LyS1 @12:20:59.84 AirS.seek evac @12:20:59.89 LyS1.seek 300ul @12:21:10.61 AirS.reconfigure AirSconfig @12:21:10.67 AirS.seek :full @12:21:24.89 LyS1.seek :empty @12:21:46.22 PuckHeater.seek 84.2C @12:22:16.53 PuckHeater.hold 15:00,84.2C @12:37:15.95 LyS1.reconfigure LyS1config @12:37:16.02 LyS1.seek 900ul @12:37:24.35 AirS.seek :tVent @12:37:24.44 delay 2 seconds @12:37:26.47 AirS.reconfigure HalfSpeedAirS @12:37:26.53 AirS.seek :evac @12:37:54.27 delay 15 seconds @12:38:09.30 AirS.reconfigure AirSconfig @12:38:09.37 AirS.seek :tVent @12:38:23.59 delay 2 seconds @12:38:25.62 AirS.reconfigure HalfSpeedAirS @12:38:25.69 AirS.seek :evac @12:38:53.44 delay 15 seconds @12:39:08.47 AirS.reconfigure AirSconfig @12:39:08.52 AirS.seek :tVent @12:39:22.73 delay 2 seconds @12:39:24.76 AirS.reconfigure HalfSpeedAirS @12:39:24.81 AirS.seek :evac @12:39:52.55 delay 15 seconds @12:40:07.58 delay 1:00 @12:41:07.59 Handoff.select :waste @12:41:07.79 delay 15 seconds @12:41:22.82 AirS.seek :fVent @12:41:25.03 delay 10 seconds @12:41:35.06 AirS.reconfigure AirSconfig @12:41:35.12 AirS.seek :evac @12:41:36.56 Lysis Recovered in LyS1 @12:41:36.57 Lysis Complete @12:41:36.58 Handoff.select :waste @12:41:36.60 LyS2.seek :full @12:41:36.69 LyS1.reconfigure DAmixLyS1 @12:41:36.75 LyS2.reconfigure DAmixLyS2 @12:41:37.28 LyS1.seek 650ul @12:41:37.33 LyS2.seek 1480ul @12:42:12.40 delay 5 seconds @12:42:17.42 Handoff.select :SPR @12:42:17.54 SPR.command "start 1 14" @12:42:19.43 Running Experiment-Rep #1 @12:42:19.44 delay 10 seconds @12:42:29.47 SPR.command "start 1 0" @12:42:32.27 Waiting up to 4:00 for SPR to enter state 1 @12:44:36.56 SPR.command "hold_on" @12:44:38.38 LyS1.seek 300ul @12:44:38.45 LyS2.seek 1130ul @12:45:27.45 delay 15 seconds @12:45:42.47 SPR.command "hold_off" @12:45:45.22 Waiting up to 25:00 for SPR to enter state 7 @13:05:49.64 Handoff.select :waste @13:05:49.76 LyS1.reconfigure LyS1config @13:05:49.82 LyS2.reconfigure LyS2config @13:05:49.89 Rep #1 SPR Analysis has Completed @13:05:50.56 Ended log /var/log/esp/spr18jul2512h.spr @13:05:50.57 SPR.command "stats_off" @13:05:51.09 Handoff.select :close @13:05:51.62
PV.seek :locked @13:05:52.62 ArS.seek :clear @13:05:52.77 LyS1.seek :clear @13:05:52.95 LyS2.seek :clear @13:05:53.14 AirS.seek :clear @13:06:10.38
ArS.coast @13:06:10.43 LyS1.coast @13:06:10.47 LyS2.coast @13:06:10.52 AirS.coast @13:06:10.56 TV.coast @13:06:10.60 SP.coast @13:06:10.65 SC.coast @13:06:10.69 PV.coast @13:06:10.74 PinPort.coast @13:06:10.93 Gate.power :motor,:OFF @13:06:10.95 Gate.power :heat,:OFF @13:06:10.96 Gate.power :main,:OFF @13:06:10.97 Gate.power :cartridge,:OFF @13:06:10.98 Gate.power :core,:OFF @13:06:10.99 Gate.power :analytic1,:OFF @13:06:11.01 Gate.power :analytic2,:OFF @13:06:11.02 Gate.power :analytic3,:OFF @13:06:11.04 Safely stopped and ready to power off @13:06:11.22 delay 30 seconds @13:06:41.36 Gate.power :main,:ON,limit to 5000mA @13:06:41.38 Gate.power :analytic1,:ON,limit to 5000mA @13:06:41.39 Gate.power :analytic2,:ON,limit to 5000mA @13:06:41.40 Gate.power :analytic3,:ON,limit to 5000mA @13:06:41.46 Powering core bus @13:06:43.84 SPR Module 1, FW Rev. B, FPGA Rev. C @13:06:44.01
ArS.configure ArSconfig @13:06:44.12 LyS1.configure LyS1config @13:06:44.26 LyS2.configure LyS2config @13:06:44.38 AirS.configure AirSconfig @13:06:44.53 TV.configure TVconfig @13:06:44.67 SP.configure SPconfig @13:06:44.81 SC.configure SCconfig @13:06:44.96 PV.configure PVconfig @13:06:45.10 PinPort.configure PPconfig @13:06:45.25 Sea.configure SPconfig @13:06:45.31 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @13:06:45.33 Gate.power :motor,:ON,limit to 5000mA @13:06:45.58 ArS.reconfigure ArSconfig @13:06:46.09 ArS.home.jog 0 @13:06:46.17 PinPort.reconfigure PPconfig @13:06:46.20 PV.reconfigure PVconfig @13:06:46.24 TV.reconfigure TVconfig @13:06:46.29 LyS2.reconfigure LyS2config @13:06:46.33 LyS1.reconfigure LyS1config @13:06:46.40 AirS.reconfigure AirSconfig @13:06:46.73 PinPort.home.jog 0 @13:06:46.78 TV.home.jog 0 @13:06:46.80 PV.home.jog 0 @13:06:46.83 LyS2.home.jog 0 @13:06:46.88 LyS1.home.jog 0 @13:06:46.91 AirS.home.jog 0 @13:06:47.80 PinPort.reconfigure HomePP @13:06:47.94 AirS.reconfigure HomeMagSyringe @13:06:47.97 TV.reconfigure HomeTV @13:06:48.02 LyS1.reconfigure HomeSyringe @13:06:48.07 ArS.reconfigure HomeMagSyringe @13:06:48.12 PV.reconfigure HomePV @13:06:48.15 LyS2.reconfigure HomeSyringe @13:06:48.47 PinPort.jog -2410 @13:06:48.53 AirS.jog -308 @13:06:48.56 TV.jog -551 @13:06:48.60 PV.jog -1499 @13:06:48.67 ArS.jog -277 @13:06:48.72 LyS1.jog -276 @13:06:49.03 LyS2.jog -264 @13:06:49.78 AirS.setRawPosition! -50 @13:06:49.83 AirS.seek :home @13:06:49.97 LyS2.setRawPosition! -50 @13:06:50.00 LyS1.setRawPosition! -50 @13:06:50.02 ArS.setRawPosition! -50 @13:06:50.16 LyS2.seek :home @13:06:50.23 LyS1.seek :home @13:06:50.26 ArS.seek :home @13:06:50.57 PV.setRawPosition! -50 @13:06:50.61 AirS.reconfigure AirSconfig @13:06:50.65 LyS2.reconfigure LyS2config @13:06:50.75 PV.seek :home @13:06:50.97 LyS1.reconfigure LyS1config @13:06:50.99 ArS.reconfigure ArSconfig @13:06:51.18 TV.setRawPosition! 0 @13:06:51.21 PV.reconfigure PVconfig @13:06:51.34 TV.seek :home @13:06:51.46 TV.reconfigure TVconfig @13:06:54.87 PinPort.setRawPosition! 0 @13:06:54.92 PinPort.seek :home @13:06:55.00 PinPort.reconfigure PPconfig @13:06:55.55
TV.seek :clear @13:06:57.16 TV.coast @13:06:57.21 SC.reconfigure HomeSC @13:06:57.27 SC.home.jog 0 @13:06:58.05 SC.reconfigure SCconfig @13:06:58.10 SC.seek 13 @13:06:58.19 Selecting Cartridge 9 @13:06:58.57 SC.seek 9 @13:06:59.73 LyS2.seek :locked @13:06:59.92 PV.seek :locked @13:07:00.09 AirS.seek :locked @13:07:04.18
Powering cartridge bus @13:07:06.31 Gate.setIR 1750,5000,0,6 @13:07:06.45 Gate.setIR 0,5000,0,6 @13:07:06.48 Loaded Archive Cartridge @13:07:06.49 Gate.power :cartridge,:OFF @13:07:06.77 Duration of filtering limited to 1:05:00 @13:07:07.07 Priming sample loop w/100ml, bypass w/10ml @13:07:07.16 SP.setPosition! 0ml @13:07:07.20 Intake.open @13:07:07.24 Exhaust.open @13:07:08.07 Sea @-42.9psia @13:07:08.09 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @13:07:14.17 Vacuum tare is 20% of full scale @13:07:14.19 PB.open @13:07:14.25 Exhaust.close @13:07:15.04 Sea @-42.9psia @13:07:15.14 SP.reconfigure SPprime @13:07:15.20 SP.seek 10ml,1:21 @13:07:26.08 SP.setPosition! 0ml @13:07:26.12 PB.close @13:07:26.16 Exhaust.open @13:07:27.00 Sea @-42.9psia @13:07:27.09 SP.reconfigure SPprime @13:07:27.16 SP.seek 100ml,8:51 @13:09:13.87 Pressurizing cartridge to 26psi @13:09:13.89 Intake.close @13:09:14.62 SP.setPosition! 0ml @13:09:14.66 PB.open @13:09:15.42 TV.seek :crack @13:09:16.22 TV.seek :clear @13:09:17.02 Intake.open @13:09:18.49 PB.close @13:09:19.20 Exhaust.close @13:09:20.00 Sea @-42.9psia @13:09:20.10 SP.reconfigure SPcharge @13:09:20.16 SP.seek 7.5ml,1:08 @13:09:25.91 Cartridge pressurized with 0.151ml to 26.0psi @13:09:26.18 delay 3 seconds @13:09:29.27 Cartridge at 14.2psi 3 seconds later @13:09:29.36 TV.seek :cartridge @13:09:31.19 TV.seek :clear @13:09:33.01 Sampling 250.0ml @13:09:33.03 SP.setPosition! 0ml @13:09:33.07 Exhaust.open @13:09:33.88 Sea @-42.9psia @13:09:33.98 SP.reconfigure SPsample @13:09:34.05 SP.seek 250ml,21:21 @13:09:35.05 Cartridge 9 sampling 0/250ml at 0.17ml/s with 5.5psi vacuum -39.0m deep (-42.9psia) @13:10:35.04 Cartridge 9 sampling 25/250ml at 0.43ml/s with 26.1psi vacuum -39.0m deep (-42.9psia) @13:11:35.04 Cartridge 9 sampling 49/250ml at 0.37ml/s with 17.5psi vacuum -39.0m deep (-42.9psia) @13:12:35.06 Cartridge 9 sampling 71/250ml at 0.35ml/s with 16.8psi vacuum -39.0m deep (-42.9psia) @13:13:35.04 Cartridge 9 sampling 91/250ml at 0.29ml/s with 25.1psi vacuum -39.0m deep (-42.9psia) @13:14:35.05 Cartridge 9 sampling 111/250ml at 0.29ml/s with 21.4psi vacuum -39.0m deep (-42.9psia) @13:15:35.04 Cartridge 9 sampling 129/250ml at 0.27ml/s with 25.3psi vacuum -39.0m deep (-42.9psia) @13:16:35.05 Cartridge 9 sampling 146/250ml at 0.26ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @13:17:35.04 Cartridge 9 sampling 163/250ml at 0.26ml/s with 25.5psi vacuum -39.0m deep (-42.9psia) @13:18:35.04 Cartridge 9 sampling 178/250ml at 0.24ml/s with 23.9psi vacuum -39.0m deep (-42.9psia) @13:19:35.05 Cartridge 9 sampling 192/250ml at 0.27ml/s with 25.9psi vacuum -39.0m deep (-42.9psia) @13:20:35.05 Cartridge 9 sampling 206/250ml at 0.22ml/s with 26.7psi vacuum -39.0m deep (-42.9psia) @13:21:35.05 Cartridge 9 sampling 219/250ml at 0.23ml/s with 21.6psi vacuum -39.0m deep (-42.9psia) @13:22:35.05 Cartridge 9 sampling 232/250ml at 0.21ml/s with 25.9psi vacuum -39.0m deep (-42.9psia) @13:23:35.04 Cartridge 9 sampling 245/250ml at 0.20ml/s with 25.7psi vacuum -39.0m deep (-42.9psia) @13:24:00.96 Intake.close @13:24:01.00 Exhaust.close @13:24:01.80 Sampled 250.0ml @13:24:01.89 TV.seek :bypass @13:24:03.52 TV.seek :clear @13:24:05.45 PV.seek :reagent @13:24:06.31 Sample loop closed. Ready to Deliver Reagents @13:24:06.33 ArS.seek :sealed @13:24:10.60 ArS.seek :evac @13:24:24.54 delay 20 seconds @13:24:44.58 ArS.seek :clear @13:25:02.30 RNAlater soak for 10:00 @13:33:23.22 Session Begun @13:33:27.47 -> showlog 100 @13:33:40.24 -> Cartridge.loaded @13:33:40.26 NoMethodError in brent -- undefined method `loaded' for Cartridge:Module @13:33:54.91 -> SC @13:35:02.35 ArS.seek :tVent @13:35:04.96 delay 1 second @13:35:05.99 ArS.seek :evac @13:35:21.63 delay 10 seconds @13:35:22.87 -> exit @13:35:22.89 Session Ended @13:35:31.66 Sample preserved and evacuated @13:35:31.84
PV.seek :locked @13:35:32.84 ArS.seek :clear @13:35:33.08 LyS2.seek :clear @13:35:33.27 AirS.seek :clear @13:35:50.62
ArS.coast @13:35:50.68 LyS1.coast @13:35:50.72 LyS2.coast @13:35:50.76 AirS.coast @13:35:50.81 TV.coast @13:35:50.85 SP.coast @13:35:50.90 SC.coast @13:35:50.94 PV.coast @13:35:50.99 PinPort.coast @13:35:51.19 Gate.power :motor,:OFF @13:35:51.20 Gate.power :main,:OFF @13:35:51.21 Gate.power :core,:OFF @13:35:51.23 Gate.power :analytic1,:OFF @13:35:51.25 Gate.power :analytic2,:OFF @13:35:51.26 Gate.power :analytic3,:OFF @13:35:51.27 Safely stopped and ready to power off @13:35:51.44 delay 30 seconds @13:36:21.56 Gate.power :main,:ON,limit to 5000mA @13:36:21.57 Gate.power :analytic1,:ON,limit to 5000mA @13:36:21.59 Gate.power :analytic2,:ON,limit to 5000mA @13:36:21.60 Gate.power :analytic3,:ON,limit to 5000mA @13:36:21.66 Powering core bus @13:36:24.06 SPR Module 1, FW Rev. B, FPGA Rev. C @13:36:24.21
ArS.configure ArSconfig @13:36:24.33 LyS1.configure LyS1config @13:36:24.48 LyS2.configure LyS2config @13:36:24.59 AirS.configure AirSconfig @13:36:24.72 TV.configure TVconfig @13:36:24.85 SP.configure SPconfig @13:36:25.00 SC.configure SCconfig @13:36:25.14 PV.configure PVconfig @13:36:25.28 PinPort.configure PPconfig @13:36:25.43 Sea.configure SPconfig @13:36:25.49 All elf controllers running firmware version 4.46 @13:36:25.52 Gate.power :motor,:ON,limit to 5000mA @13:36:25.73 ArS.reconfigure ArSconfig @13:36:26.09 ArS.home.jog 0 @13:36:26.33 PV.reconfigure PVconfig @13:36:26.38 TV.reconfigure TVconfig @13:36:26.41 LyS2.reconfigure LyS2config @13:36:26.47 LyS1.reconfigure LyS1config @13:36:26.55 PinPort.reconfigure PPconfig @13:36:26.62 AirS.reconfigure AirSconfig @13:36:27.27 PV.home.jog 0 @13:36:27.32 TV.home.jog 0 @13:36:27.38 LyS2.home.jog 0 @13:36:27.42 PinPort.home.jog 0 @13:36:27.48 LyS1.home.jog 0 @13:36:27.51 AirS.home.jog 0 @13:36:28.09 ArS.reconfigure HomeMagSyringe @13:36:28.18 ArS.jog -278 @13:36:28.39 PinPort.reconfigure HomePP @13:36:28.47 PV.reconfigure HomePV @13:36:28.60 TV.reconfigure HomeTV @13:36:28.62 LyS2.reconfigure HomeSyringe @13:36:28.79 PinPort.jog -108 @13:36:28.86 PV.jog -1496 @13:36:28.92 LyS2.jog -265 @13:36:28.96 TV.jog -552 @13:36:29.22 ArS.setRawPosition! -50 @13:36:29.28 LyS1.reconfigure HomeSyringe @13:36:29.30 AirS.reconfigure HomeMagSyringe @13:36:29.48 ArS.seek :home @13:36:29.50 LyS1.jog -272 @13:36:29.54 AirS.jog -297 @13:36:29.77 PinPort.setRawPosition! 0 @13:36:29.80 LyS2.setRawPosition! -50 @13:36:29.89 PinPort.seek :home @13:36:29.93 LyS2.seek :home @13:36:30.14 ArS.reconfigure ArSconfig @13:36:30.17 PinPort.reconfigure PPconfig @13:36:30.39 AirS.setRawPosition! -50 @13:36:30.44 LyS2.reconfigure LyS2config @13:36:30.46 PV.setRawPosition! -50 @13:36:30.49 LyS1.setRawPosition! -50 @13:36:30.66 AirS.seek :home @13:36:30.69 PV.seek :home @13:36:30.72 LyS1.seek :home @13:36:31.06 TV.setRawPosition! 0 @13:36:31.13 TV.seek :home @13:36:31.31 TV.reconfigure TVconfig @13:36:31.36 AirS.reconfigure AirSconfig @13:36:31.39 LyS1.reconfigure LyS1config @13:36:31.43 PV.reconfigure PVconfig @13:36:32.15
TV.seek :clear @13:36:33.77 TV.coast @13:36:33.83 SC.reconfigure HomeSC @13:36:33.88 SC.home.jog 0 @13:36:34.66 SC.reconfigure SCconfig @13:36:34.71 SC.seek 9 @13:36:34.80 Selecting Cartridge 8 @13:36:35.18 SC.seek 8 @13:36:36.08 LyS2.seek :locked @13:36:36.27 PV.seek :locked @13:36:36.45 AirS.seek :locked @13:36:40.55
Powering cartridge bus @13:36:42.69 Gate.setIR 1750,5000,0,6 @13:36:42.82 Gate.setIR 0,5000,0,6 @13:36:42.88 Loaded Lyse-n-Go Cartridge @13:36:42.89 PuckHeater.configure HeatConfig @13:36:42.93 Gate.power :heat,:ON,limit to 5000mA @13:36:43.25 Duration of filtering limited to 1:05:00 @13:36:43.55 Priming sample loop w/100ml, bypass w/10ml @13:36:43.64 SP.setPosition! 0ml @13:36:43.68 Intake.open @13:36:43.71 Exhaust.open @13:36:44.57 Sea @-42.9psia @13:36:44.58 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @13:36:50.35 Vacuum tare is 20% of full scale @13:36:50.37 PB.open @13:36:50.41 Exhaust.close @13:36:51.31 Sea @-42.9psia @13:36:51.40 SP.reconfigure SPprime @13:36:51.46 SP.seek 10ml,1:21 @13:37:02.33 SP.setPosition! 0ml @13:37:02.39 PB.close @13:37:02.42 Exhaust.open @13:37:03.32 Sea @-42.9psia @13:37:03.42 SP.reconfigure SPprime @13:37:03.48 SP.seek 100ml,8:51 @13:38:50.83 Pressurizing cartridge to 26psi @13:38:50.85 Intake.close @13:38:51.57 SP.setPosition! 0ml @13:38:51.61 PB.open @13:38:52.45 TV.seek :crack @13:38:53.27 TV.seek :clear @13:38:54.06 Intake.open @13:38:55.49 PB.close @13:38:56.26 Exhaust.close @13:38:57.07 Sea @-42.9psia @13:38:57.17 SP.reconfigure SPcharge @13:38:57.23 SP.seek 7.5ml,1:08 @13:39:09.03 Cartridge pressurized with 0.650ml to 26.3psi @13:39:09.29 delay 3 seconds @13:39:12.39 Cartridge at 23.9psi 3 seconds later @13:39:12.48 TV.seek :cartridge @13:39:14.29 TV.seek :clear @13:39:16.12 Sampling 250.0ml @13:39:16.14 SP.setPosition! 0ml @13:39:16.17 Exhaust.open @13:39:16.97 Sea @-42.9psia @13:39:17.08 SP.reconfigure SPsample @13:39:17.15 SP.seek 250ml,21:21 @13:39:18.12 Cartridge 8 sampling 0/250ml at 0.17ml/s with 3.6psi vacuum -39.0m deep (-42.9psia) @13:40:18.13 Cartridge 8 sampling 36/250ml at 0.60ml/s with 23.6psi vacuum -39.0m deep (-42.9psia) @13:40:30.11 Session Begun @13:40:36.14 -> Sea.status @13:40:46.44 -> Sea.psia @13:41:18.13 Cartridge 8 sampling 71/250ml at 0.58ml/s with 19.3psi vacuum -39.0m deep (-42.9psia) @13:42:00.59 -> Sea.status.deltaPress @13:42:18.13 Cartridge 8 sampling 106/250ml at 0.58ml/s with 23.3psi vacuum -39.0m deep (-42.9psia) @13:42:31.91 -> Sea.status.deltaPress @13:43:18.12 Cartridge 8 sampling 142/250ml at 0.57ml/s with 24.6psi vacuum -39.0m deep (-42.9psia) @13:44:13.79 -> list Sampler/:logTextBase @13:44:18.13 Cartridge 8 sampling 176/250ml at 0.57ml/s with 24.7psi vacuum -39.0m deep (-42.9psia) @13:45:18.13 Cartridge 8 sampling 211/250ml at 0.56ml/s with 24.7psi vacuum -39.0m deep (-42.9psia) @13:46:18.14 Cartridge 8 sampling 245/250ml at 0.56ml/s with 22.0psi vacuum -39.0m deep (-42.9psia) @13:46:26.91 Intake.close @13:46:26.95 Exhaust.close @13:46:27.79 Sampled 250.0ml @13:46:27.91 TV.seek :bypass @13:46:29.56 TV.seek :clear @13:46:33.05 SPR.command "read_sequences" @13:46:33.11 SPR.command "stats_on" @13:46:33.16 SPR.command "start 2 0" @13:46:35.16 Begun log /var/log/esp/spr18jul2513h.spr @13:46:35.92 Handoff.select :waste @13:46:36.08 ArS.seek empty @13:46:53.81 PinPort.seek :cartridge @13:46:59.57 LyS1.seek 1350ul @13:47:05.54 PV.seek :reagent @13:47:06.42 AirS.seek :tVent @13:47:08.51 delay 2 seconds @13:47:10.54 AirS.reconfigure EvacAirS @13:47:10.62 AirS.seek :evac @13:47:32.95 delay 15 seconds @13:47:47.98 AirS.reconfigure AirSconfig @13:47:48.08 AirS.seek :tVent @13:47:55.90 -> Sampler.seaPressure @13:48:02.31 delay 2 seconds @13:48:04.34 AirS.reconfigure EvacAirS @13:48:04.46 AirS.seek :evac @13:48:26.77 delay 15 seconds @13:48:41.81 AirS.reconfigure AirSconfig @13:48:41.89 AirS.seek :tVent @13:48:56.14 delay 2 seconds @13:48:58.17 AirS.reconfigure EvacAirS @13:48:58.25 AirS.seek :evac @13:49:20.61 delay 15 seconds @13:49:35.64 AirS.reconfigure AirSconfig @13:49:35.72 AirS.seek :tVent @13:49:49.96 delay 2 seconds @13:49:51.99 AirS.reconfigure EvacAirS @13:49:52.08 AirS.seek :evac @13:50:14.39 delay 15 seconds @13:50:17.87 -> Sampler.logText @13:50:29.42 delay 1:30 @13:51:59.45 AirS.reconfigure AirSconfig @13:51:59.53 AirS.seek :fVent @13:52:00.99 delay 15 seconds @13:52:16.02 LyS1.reconfigure SlowLyS1 @13:52:16.16 LyS1.seek 1050ul @13:52:37.25 delay 5 seconds @13:52:42.27 Handoff.select :blocked @13:52:42.51 LyS1.seek 450ul @13:53:24.54 delay 10 seconds @13:53:34.57 AirS.reconfigure SlowAirS @13:53:34.71 AirS.reconfigure to mix into 1.37806 parts LyS1 @13:53:35.28 AirS.seek evac @13:53:35.31 LyS1.seek 300ul @13:53:46.08 AirS.reconfigure AirSconfig @13:53:46.16 AirS.seek :full @13:54:00.40 LyS1.seek :empty @13:54:21.54 PuckHeater.seek 84.3C @13:54:21.74 SPR.command "start 1 14" @13:54:24.59 Ended log /var/log/esp/spr18jul2513h.spr @13:54:24.61 SPR.command "stats_off" @13:54:25.05 I2C::NodeOffline in PROCESSING -- cart Not Responding while reading Puck Heater's status @13:54:25.09 Thread::ChildDied in MAIN -- :PROCESSING @13:54:25.14 Thread::ChildDied in MAIN_scheduler -- :MAIN @13:54:25.28 Session Ended @14:04:40.06PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @14:04:40.09 >BREAK< ESP3 Gateway v0.99 for cartridge bus @14:04:41.67 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @14:04:43.33 Polling Can every 5:00 @14:04:43.44 Gate.power :analytic1,:ON,limit to 5000mA @14:04:43.45 Gate.power :analytic2,:ON,limit to 5000mA @14:04:43.46 Gate.power :analytic3,:ON,limit to 5000mA @14:04:44.27 ArS.configure ArSconfig @14:04:44.49 LyS1.configure LyS1config @14:04:44.69 SPR Module 1, FW Rev. B, FPGA Rev. C @14:04:44.86
LyS2.configure LyS2config @14:04:44.98 AirS.configure AirSconfig @14:04:45.13 TV.configure TVconfig @14:04:45.27 SP.configure SPconfig @14:04:45.41 SC.reconfigure SCconfig @14:04:45.46 SC.hold @14:04:45.52 SC.configure SCconfig @14:04:45.65 PV.configure PVconfig @14:04:45.78 PinPort.configure PPconfig @14:04:45.87 PuckHeater.coast @14:04:45.93 PuckHeater.configure HeatConfig @14:04:46.07 Sea.configure SPconfig @14:04:46.13 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @14:04:46.21 Intake.close @14:04:46.26 Exhaust.close @14:04:46.32 PB.close @14:04:46.54
Loading /home/esp/esp2/mission/processAll.rb mission @14:04:46.60 Processing all remaining dry cartridges in: {-500 => 23, -250 => 8, 1 => :spares, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 250 => [9, 13, 18], 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2], wet: 56} @14:04:46.61 Sampling maximum of 250.0ml per cartridge @14:04:46.62 Fixing samples for 10:00, evacuating once @14:04:46.64 delay 30 seconds @14:04:46.95 Session Begun @14:05:17.40
SC.seek 8 @14:05:17.51 Selecting Cartridge 2 @14:05:17.78 Gate.power :heat,:OFF @14:05:17.79 Gate.power :cartridge,:OFF @14:05:17.95 ArS.seek :clear @14:05:18.17 LyS1.seek :clear @14:05:18.34 AirS.seek :clear @14:05:18.64 PinPort.seek :clear @14:05:36.08 LyS2.seek :clear @14:05:36.28 PV.seek :clear @14:05:39.00
SC.seek 2 @14:05:40.61 LyS2.seek :locked @14:05:40.81 PV.seek :locked @14:05:41.02 AirS.seek :locked @14:05:45.13
Powering cartridge bus @14:05:47.28 Gate.setIR 1750,5000,0,6 @14:05:47.40 Gate.setIR 0,5000,0,6 @14:05:47.46 Loaded Archive Cartridge @14:05:47.47 Gate.power :cartridge,:OFF @14:05:47.81 Duration of filtering limited to 1:05:00 @14:05:48.46 Priming sample loop w/100ml, bypass w/10ml @14:05:48.59 SP.setPosition! 0ml @14:05:48.65 Intake.open @14:05:48.69 Exhaust.open @14:05:49.64 Sea @-42.9psia @14:05:49.66 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @14:05:55.45 Vacuum tare is 20% of full scale @14:05:55.47 PB.open @14:05:55.53 Exhaust.close @14:05:56.44 Sea @-42.9psia @14:05:56.57 SP.reconfigure SPprime @14:05:56.66 SP.seek 10ml,1:21 @14:06:07.56 SP.setPosition! 0ml @14:06:07.64 PB.close @14:06:07.68 Exhaust.open @14:06:08.56 Sea @-42.9psia @14:06:08.69 SP.reconfigure SPprime @14:06:08.79 SP.seek 100ml,8:51 @14:07:40.63 -> Cmd.slots @14:07:54.72 Pressurizing cartridge to 26psi @14:07:54.73 Intake.close @14:07:55.56 SP.setPosition! 0ml @14:07:55.63 PB.open @14:07:56.47 TV.seek :crack @14:07:57.31 TV.seek :clear @14:07:58.13 Intake.open @14:07:59.56 PB.close @14:08:00.29 Exhaust.close @14:08:01.10 Sea @-42.9psia @14:08:01.22 SP.reconfigure SPcharge @14:08:01.32 SP.seek 7.5ml,1:08 @14:08:07.26 Cartridge pressurized with 0.159ml to 26.8psi @14:08:07.54 Pressure peaked at 27.3psi @14:08:07.56 delay 3 seconds @14:08:10.66 Cartridge at 15.9psi 3 seconds later @14:08:10.78 TV.seek :cartridge @14:08:12.63 TV.seek :clear @14:08:14.48 Sampling 250.0ml @14:08:14.50 SP.setPosition! 0ml @14:08:14.58 Exhaust.open @14:08:15.40 Sea @-42.9psia @14:08:15.54 SP.reconfigure SPsample @14:08:15.63 SP.seek 250ml,21:21 @14:08:16.57 Cartridge 2 sampling 0/250ml at 0.15ml/s with 5.1psi vacuum -39.0m deep (-42.9psia) @14:09:16.58 Cartridge 2 sampling 25/250ml at 0.41ml/s with 25.6psi vacuum -39.0m deep (-42.9psia) @14:10:16.57 Cartridge 2 sampling 49/250ml at 0.35ml/s with 22.1psi vacuum -39.0m deep (-42.9psia) @14:10:54.77 Session Begun @14:10:59.64 -> Sea.absPress @14:11:02.78 -> Sea.absPress @14:11:16.57 Cartridge 2 sampling 71/250ml at 0.38ml/s with 26.8psi vacuum -39.0m deep (-42.9psia) @14:12:16.58 Cartridge 2 sampling 92/250ml at 0.33ml/s with 27.2psi vacuum -39.0m deep (-42.9psia) @14:12:32.15 -> class < 7 then psi = psia pressCnts; txt<<" %.1fm deep (%.1fpsia)" % [depth(psi), psi]; end; txt; end @14:12:32.18 SyntaxError in brent -- compile error brent:3: syntax error, unexpected $end, expecting kEND ...epth(psi), psi]; end; txt; end ^ @14:13:16.58 Cartridge 2 sampling 111/250ml at 0.29ml/s with 24.4psi vacuum -39.0m deep (-42.9psia) @14:13:37.77 -> class < 7; psi = psia pressCnts; txt<<" %.1fm deep (%.1fpsia)" % [depth(psi), psi]; end; txt; end @14:13:38.15 SyntaxError in brent -- compile error brent:4: syntax error, unexpected $end, expecting kEND ...epth(psi), psi]; end; txt; end ^ @14:14:05.92 -> Sampler.logText @14:14:16.61 Cartridge 2 sampling 130/250ml at 0.33ml/s with 21.9psi vacuum -39.0m deep (-42.9psia) @14:14:48.69 -> class < 7 then psi = psia pressCnts; txt<<" %.1fm deep (%.1fpsia)" % [depth(psi), psi]; end; txt; end; end @14:14:52.96 -> Sampler.logText @14:14:57.60 UserAbort in brent -- @14:16:15.28 -> class < 7 then psi = psia pressCnts; txt<<" %.1fm deep (%.1fpsia)" % [depth(psi), psi]; end; txt; end; end @14:16:24.34 sampling 165/250ml at 0.27ml/s with 25.7psi vacuum -38.9m deep (-42.9psia) @14:16:24.42 Schedule::EventInPast in sampling -- Delay::RunEvent @14:16:16.43PDT25-Jul-18 is in the past @14:16:45.12 -> Sampler.logText @14:17:59.52 -> showlog 30 @14:18:26.08 -> Cmd.slots @14:18:40.05 -> Cmd.slots 2=>:dry @14:18:46.15 -> MainThred.exit @14:18:46.17 NameError in brent -- uninitialized constant MainThred @14:18:48.67 -> MainThread.exit @14:18:48.70
SP.stop @14:18:48.90 Intake.close @14:18:49.01 Exhaust.close @14:18:49.30 Exhaust.close @14:18:49.34 Intake.close @14:18:49.56 Gripper::Error in closeExhaust -- Exhaust close aborted @14:18:49.61 Gripper::Error in closeIntake -- Intake close aborted @14:18:49.86 TV.seek :bypass @14:18:51.52 TV.seek :clear @14:18:59.38 I2C::Request::Timeout in closeExhaust -- No Response to I2C::Servo::Grip3Request[60:->48] @14:18:59.40 Retry #1 of 3 @14:18:59.55 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[60:48->20] aborted @14:18:59.60 I2C::Request::Timeout in closeIntake -- No Response to I2C::Servo::Grip3Request[61:->48] @14:18:59.63 Retry #1 of 3 @14:18:59.76 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[61:48->20] aborted @14:19:07.85 -> MainThread.exit @14:19:09.48 I2C::Request::Timeout in closeExhaust -- No Response to I2C::Servo::Grip3Request[66:->48] @14:19:09.50 Retry #2 of 3 @14:19:09.63 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[66:48->20] aborted @14:19:10.18 I2C::Request::Timeout in closeIntake -- No Response to I2C::Servo::Grip3Request[67:->48] @14:19:10.22 Retry #2 of 3 @14:19:10.35 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[67:48->20] aborted @14:19:17.99 -> MainThread.exit @14:19:19.57 I2C::Request::Timeout in closeExhaust -- No Response to I2C::Servo::Grip3Request[63:->48] @14:19:19.63 Retry #3 of 3 @14:19:19.77 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[63:48->20] aborted @14:19:20.30 I2C::Request::Timeout in closeIntake -- No Response to I2C::Servo::Grip3Request[69:->48] @14:19:20.32 Retry #3 of 3 @14:19:20.44 I2C::UnexpectedReply in core -- I2C::Servo::Grip3Reply[69:48->20] aborted @14:19:21.31 -> MainThread.exit @14:19:26.37 -> MainThread.exit @14:19:29.72 I2C::Request::Timeout in closeExhaust -- Exhaust could not finish closing because No Response to I2C::Servo::Grip3Request[6A:->48] @14:19:30.40 I2C::Request::Timeout in closeIntake -- Intake could not finish closing because No Response to I2C::Servo::Grip3Request[5E:->48] @14:19:30.49 Session Ended @14:19:30.54 Session Ended @14:24:43.84PDT25-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @14:24:43.86 >BREAK< ESP3 Gateway v0.99 for cartridge bus @14:24:45.66 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @14:24:47.35 Polling Can every 5:00 @14:24:47.51 Gate.power :analytic1,:ON,limit to 5000mA @14:24:47.53 Gate.power :analytic2,:ON,limit to 5000mA @14:24:47.55 Gate.power :analytic3,:ON,limit to 5000mA @14:24:48.29 ArS.configure ArSconfig @14:24:48.42 LyS1.configure LyS1config @14:24:48.58 LyS2.configure LyS2config @14:24:48.65 SPR Module 1, FW Rev. B, FPGA Rev. C @14:24:48.75
AirS.configure AirSconfig @14:24:48.88 TV.configure TVconfig @14:24:49.01 SP.configure SPconfig @14:24:49.17 SC.configure SCconfig @14:24:49.30 PV.configure PVconfig @14:24:49.45 PinPort.configure PPconfig @14:24:49.59 Sea.configure SPconfig @14:24:49.65 All elf controllers running firmware version 4.46 @14:24:49.72 Intake.close @14:24:49.76 Exhaust.close @14:24:49.85 PB.close @14:24:50.07
Loading /home/esp/esp2/mission/processAll.rb mission @14:24:50.12 Processing all remaining dry cartridges in: {-500 => 23, -250 => 8, 1 => :spares, 1.391 => 28, 2 => :spareGeneric, 248.019 => 29, 250 => [9, 13, 18], 500 => [32, 34, 37, 40, 43, 46, 49, 52, 55], dry: [1, 2], wet: 56} @14:24:50.13 Sampling maximum of 250.0ml per cartridge @14:24:50.23 Fixing samples for 10:00, evacuating once @14:24:50.28 delay 30 seconds @14:24:50.34 Session Begun @14:25:20.48 ArS.reconfigure ArSconfig @14:25:20.86 ArS.home.jog 0 @14:25:21.19 TV.reconfigure TVconfig @14:25:21.22 AirS.reconfigure AirSconfig @14:25:21.26 LyS1.reconfigure LyS1config @14:25:21.34 PinPort.reconfigure PPconfig @14:25:21.41 PV.reconfigure PVconfig @14:25:21.49 LyS2.reconfigure LyS2config @14:25:21.74 TV.home.jog 0 @14:25:21.82 AirS.home.jog 0 @14:25:21.87 LyS1.home.jog 0 @14:25:21.90 PinPort.home.jog 0 @14:25:21.96 PV.home.jog 0 @14:25:22.05 LyS2.home.jog 0 @14:25:22.71 ArS.reconfigure HomeMagSyringe @14:25:23.01 ArS.jog -281 @14:25:23.09 PV.reconfigure HomePV @14:25:23.15 AirS.reconfigure HomeMagSyringe @14:25:23.20 LyS2.reconfigure HomeSyringe @14:25:23.25 LyS1.reconfigure HomeSyringe @14:25:23.32 TV.reconfigure HomeTV @14:25:23.39 PinPort.reconfigure HomePP @14:25:23.75 PV.jog -1491 @14:25:23.80 AirS.jog -2051 @14:25:23.85 LyS2.jog -1442 @14:25:23.91 LyS1.jog -234 @14:25:23.95 TV.jog -550 @14:25:24.04 PinPort.jog -109 @14:25:24.41 ArS.setRawPosition! -50 @14:25:24.48 ArS.seek :home @14:25:24.62 PinPort.setRawPosition! 0 @14:25:24.69 PinPort.seek :home @14:25:24.89 PinPort.reconfigure PPconfig @14:25:25.20 LyS1.setRawPosition! -50 @14:25:25.32 ArS.reconfigure ArSconfig @14:25:25.48 LyS1.seek :home @14:25:25.68 AirS.setRawPosition! -50 @14:25:25.71 LyS2.setRawPosition! -50 @14:25:25.75 PV.setRawPosition! -50 @14:25:25.95 AirS.seek :home @14:25:26.00 PV.seek :home @14:25:26.05 LyS2.seek :home @14:25:26.35 TV.setRawPosition! 0 @14:25:26.43 LyS1.reconfigure LyS1config @14:25:26.58 TV.seek :home @14:25:26.80 TV.reconfigure TVconfig @14:25:26.85 AirS.reconfigure AirSconfig @14:25:26.91 PV.reconfigure PVconfig @14:25:26.96 LyS2.reconfigure LyS2config @14:25:27.69
TV.seek :clear @14:25:29.32 TV.coast @14:25:29.41 SC.reconfigure HomeSC @14:25:29.50 SC.home.jog 0 @14:25:30.30 SC.reconfigure SCconfig @14:25:30.38 SC.seek 2 @14:25:30.49 Selecting Cartridge 2 @14:25:30.77 LyS2.seek :locked @14:25:30.96 PV.seek :locked @14:25:31.17 AirS.seek :locked @14:25:35.30
Powering cartridge bus @14:25:37.43 Gate.setIR 1750,5000,0,6 @14:25:37.56 Gate.setIR 0,5000,0,6 @14:25:37.61 Loaded Archive Cartridge @14:25:37.63 Gate.power :cartridge,:OFF @14:25:37.99 Duration of filtering limited to 1:05:00 @14:25:38.35 Priming sample loop w/100ml, bypass w/10ml @14:25:38.47 SP.setPosition! 0ml @14:25:38.54 Intake.open @14:25:38.57 Exhaust.open @14:25:39.46 Sea @-42.9psia @14:25:39.48 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @14:25:45.23 Vacuum tare is 20% of full scale @14:25:45.26 PB.open @14:25:45.31 Exhaust.close @14:25:46.18 Sea @-42.9psia @14:25:46.30 SP.reconfigure SPprime @14:25:46.39 SP.seek 10ml,1:21 @14:25:49.46 -> Cartridge.type @14:25:57.29 SP.setPosition! 0ml @14:25:57.36 PB.close @14:25:57.41 Exhaust.open @14:25:58.62 Sea @-42.9psia @14:25:58.74 SP.reconfigure SPprime @14:25:58.84 SP.seek 100ml,8:51 @14:27:31.06 -> Sampler.logText @14:27:45.39 Pressurizing cartridge to 26psi @14:27:45.40 Intake.close @14:27:46.18 SP.setPosition! 0ml @14:27:46.25 PB.open @14:27:47.09 TV.seek :crack @14:27:47.94 TV.seek :clear @14:27:48.76 Intake.open @14:27:50.19 PB.close @14:27:50.95 Exhaust.close @14:27:51.77 Sea @-42.9psia @14:27:51.89 SP.reconfigure SPcharge @14:27:51.98 SP.seek 7.5ml,1:08 @14:27:55.98 Cartridge pressurized with 0.070ml to 26.1psi @14:27:56.26 delay 3 seconds @14:27:59.35 Cartridge at 15.9psi 3 seconds later @14:27:59.47 TV.seek :cartridge @14:28:01.32 TV.seek :clear @14:28:03.16 Sampling 250.0ml @14:28:03.18 SP.setPosition! 0ml @14:28:03.27 Exhaust.open @14:28:04.09 Sea @-42.8psia @14:28:04.22 SP.reconfigure SPsample @14:28:04.32 SP.seek 250ml,21:21 @14:28:05.26 Cartridge 2 sampling 0/250ml at 0.16ml/s with 11.3psi vacuum @14:29:05.26 Cartridge 2 sampling 14/250ml at 0.23ml/s with 20.0psi vacuum @14:30:05.27 Cartridge 2 sampling 28/250ml at 0.24ml/s with 26.1psi vacuum @14:31:05.27 Cartridge 2 sampling 41/250ml at 0.21ml/s with 20.1psi vacuum @14:32:05.26 Cartridge 2 sampling 53/250ml at 0.20ml/s with 27.8psi vacuum @14:33:05.27 Cartridge 2 sampling 66/250ml at 0.20ml/s with 25.9psi vacuum @14:34:05.27 Cartridge 2 sampling 78/250ml at 0.20ml/s with 27.5psi vacuum @14:35:03.17 Session Begun @14:35:05.26 Cartridge 2 sampling 90/250ml at 0.20ml/s with 28.8psi vacuum @14:36:05.26 Cartridge 2 sampling 102/250ml at 0.20ml/s with 33.0psi vacuum @14:37:05.26 Cartridge 2 sampling 114/250ml at 0.20ml/s with 33.1psi vacuum @14:37:49.35 Flow averaged 0.21ml/s over 9:42 @14:37:49.42 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @14:37:50.10 Relieved excess vacuum in 4.6 @14:37:50.13 SP.seek 250ml,11:04 @14:37:55.59 Flow estimate reduced to 0.17ml/s @14:37:55.62 Intake.close @14:37:55.70 Exhaust.close @14:37:56.56 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:37:56.72 Sampled 124.3ml @14:37:56.86 TV.seek :bypass @14:37:58.52 TV.seek :clear @14:38:00.51 PV.seek :reagent @14:38:01.39 Sample loop closed. Ready to Deliver Reagents @14:38:01.42 ArS.seek :sealed @14:38:06.13 ArS.seek :evac @14:38:20.12 delay 20 seconds @14:38:40.16 ArS.seek :clear @14:38:57.92 RNAlater soak for 10:00 @14:44:03.80 -> exit @14:44:03.81 Session Ended @14:48:57.95 ArS.seek :tVent @14:49:00.56 delay 1 second @14:49:01.59 ArS.seek :evac @14:49:17.22 delay 10 seconds @14:49:27.26 Sample preserved and evacuated @14:49:27.46
PV.seek :locked @14:49:28.50 ArS.seek :clear @14:49:28.77 LyS2.seek :clear @14:49:28.93 AirS.seek :clear @14:49:46.27
ArS.coast @14:49:46.34 LyS1.coast @14:49:46.42 LyS2.coast @14:49:46.49 AirS.coast @14:49:46.58 TV.coast @14:49:46.65 SP.coast @14:49:46.72 SC.coast @14:49:46.78 PV.coast @14:49:46.87 PinPort.coast @14:49:47.10 Gate.power :motor,:OFF @14:49:47.12 Gate.power :main,:OFF @14:49:47.16 Gate.power :core,:OFF @14:49:47.20 Gate.power :analytic1,:OFF @14:49:47.24 Gate.power :analytic2,:OFF @14:49:47.30 Gate.power :analytic3,:OFF @14:49:47.32 Safely stopped and ready to power off @14:49:47.55 delay 30 seconds @14:50:17.68 Gate.power :main,:ON,limit to 5000mA @14:50:17.70 Gate.power :analytic1,:ON,limit to 5000mA @14:50:17.77 Gate.power :analytic2,:ON,limit to 5000mA @14:50:17.78 Gate.power :analytic3,:ON,limit to 5000mA @14:50:17.89 Powering core bus @14:50:20.27 SPR Module 1, FW Rev. B, FPGA Rev. C @14:50:20.47
ArS.configure ArSconfig @14:50:20.61 LyS1.configure LyS1config @14:50:20.78 LyS2.configure LyS2config @14:50:20.92 AirS.configure AirSconfig @14:50:21.09 TV.configure TVconfig @14:50:21.26 SP.configure SPconfig @14:50:21.43 SC.configure SCconfig @14:50:21.60 PV.configure PVconfig @14:50:21.77 PinPort.configure PPconfig @14:50:21.94 Sea.configure SPconfig @14:50:22.02 All elf controllers running firmware version 4.46 @14:50:22.05 Gate.power :motor,:ON,limit to 5000mA @14:50:22.84 ArS.reconfigure ArSconfig @14:50:22.86 PV.reconfigure PVconfig @14:50:22.92 TV.reconfigure TVconfig @14:50:22.98 AirS.reconfigure AirSconfig @14:50:23.03 LyS2.reconfigure LyS2config @14:50:23.09 LyS1.reconfigure LyS1config @14:50:23.16 PinPort.reconfigure PPconfig @14:50:23.43 ArS.home.jog 0 @14:50:23.56 TV.home.jog 0 @14:50:23.66 PinPort.home.jog 0 @14:50:23.70 PV.home.jog 0 @14:50:23.76 LyS2.home.jog 0 @14:50:23.81 AirS.home.jog 0 @14:50:23.86 LyS1.home.jog 0 @14:50:25.36 PV.reconfigure HomePV @14:50:25.43 TV.reconfigure HomeTV @14:50:25.50 AirS.reconfigure HomeMagSyringe @14:50:25.56 LyS1.reconfigure HomeSyringe @14:50:25.60 PinPort.reconfigure HomePP @14:50:25.68 LyS2.reconfigure HomeSyringe @14:50:25.74 ArS.reconfigure HomeMagSyringe @14:50:26.05 PV.jog -1499 @14:50:26.16 LyS1.jog -269 @14:50:26.18 TV.jog -548 @14:50:26.25 AirS.jog -300 @14:50:26.32 PinPort.jog -109 @14:50:26.36 ArS.jog -276 @14:50:26.46 LyS2.jog -265 @14:50:27.00 PinPort.setRawPosition! 0 @14:50:27.11 PinPort.seek :home @14:50:27.32 AirS.setRawPosition! -50 @14:50:27.37 LyS2.setRawPosition! -50 @14:50:27.40 PinPort.reconfigure PPconfig @14:50:27.46 LyS1.setRawPosition! -50 @14:50:27.52 ArS.setRawPosition! -50 @14:50:27.70 AirS.seek :home @14:50:27.81 LyS2.seek :home @14:50:28.08 ArS.seek :home @14:50:28.14 LyS1.seek :home @14:50:28.38 PV.setRawPosition! -50 @14:50:28.42 AirS.reconfigure AirSconfig @14:50:28.48 LyS2.reconfigure LyS2config @14:50:28.66 PV.seek :home @14:50:28.88 TV.setRawPosition! 0 @14:50:28.91 LyS1.reconfigure LyS1config @14:50:28.94 ArS.reconfigure ArSconfig @14:50:29.08 TV.seek :home @14:50:29.38 TV.reconfigure TVconfig @14:50:29.40 PV.reconfigure PVconfig @14:50:29.80
TV.seek :clear @14:50:31.45 TV.coast @14:50:31.53 SC.reconfigure HomeSC @14:50:31.62 SC.home.jog 0 @14:50:32.40 SC.reconfigure SCconfig @14:50:32.49 SC.seek 2 @14:50:32.60 Selecting Cartridge 1 @14:50:33.04 SC.seek 1 @14:50:33.61 LyS2.seek :locked @14:50:33.80 PV.seek :locked @14:50:33.99 AirS.seek :locked @14:50:38.14
Powering cartridge bus @14:50:40.29 Gate.setIR 1750,5000,0,6 @14:50:40.42 Gate.setIR 0,5000,0,6 @14:50:40.50 Loaded Lyse-n-Go Cartridge @14:50:40.51 PuckHeater.configure HeatConfig @14:50:40.95 Gate.power :heat,:ON,limit to 5000mA @14:50:41.29 Duration of filtering limited to 1:05:00 @14:50:41.62 Priming sample loop w/100ml, bypass w/10ml @14:50:41.74 SP.setPosition! 0ml @14:50:41.81 Intake.open @14:50:41.85 Exhaust.open @14:50:42.72 Sea @-42.9psia @14:50:42.73 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @14:50:48.51 Vacuum tare is 20% of full scale @14:50:48.53 PB.open @14:50:48.59 Exhaust.close @14:50:49.48 Sea @-42.9psia @14:50:49.63 SP.reconfigure SPprime @14:50:49.74 SP.seek 10ml,1:21 @14:51:00.63 SP.setPosition! 0ml @14:51:00.71 PB.close @14:51:00.75 Exhaust.open @14:51:01.67 Sea @-42.9psia @14:51:01.79 SP.reconfigure SPprime @14:51:01.89 SP.seek 100ml,8:51 @14:52:48.48 Pressurizing cartridge to 26psi @14:52:48.49 Intake.close @14:52:49.28 SP.setPosition! 0ml @14:52:49.35 PB.open @14:52:50.15 TV.seek :crack @14:52:50.97 TV.seek :clear @14:52:51.81 Intake.open @14:52:53.22 PB.close @14:52:54.00 Exhaust.close @14:52:54.81 Sea @-42.9psia @14:52:54.94 SP.reconfigure SPcharge @14:52:55.03 SP.seek 7.5ml,1:08 @14:53:00.51 Cartridge pressurized with 0.136ml to 26.2psi @14:53:00.80 Pressure peaked at 26.3psi @14:53:00.83 delay 3 seconds @14:53:03.94 Cartridge at 18.8psi 3 seconds later @14:53:04.05 TV.seek :cartridge @14:53:05.90 TV.seek :clear @14:53:07.75 Sampling 250.0ml @14:53:07.76 SP.setPosition! 0ml @14:53:07.86 Exhaust.open @14:53:08.64 Sea @-42.9psia @14:53:08.76 SP.reconfigure SPsample @14:53:08.87 SP.seek 250ml,21:21 @14:53:09.80 Cartridge 1 sampling 0/250ml at 0.15ml/s with 2.2psi vacuum @14:54:09.80 Cartridge 1 sampling 35/250ml at 0.57ml/s with 17.3psi vacuum @14:55:09.80 Cartridge 1 sampling 70/250ml at 0.56ml/s with 25.2psi vacuum @14:56:09.81 Cartridge 1 sampling 105/250ml at 0.57ml/s with 19.5psi vacuum @14:57:09.81 Cartridge 1 sampling 139/250ml at 0.55ml/s with 16.5psi vacuum @14:58:09.84 Cartridge 1 sampling 172/250ml at 0.59ml/s with 24.2psi vacuum @14:59:09.81 Cartridge 1 sampling 206/250ml at 0.53ml/s with 16.9psi vacuum @15:00:09.81 Cartridge 1 sampling 239/250ml at 0.53ml/s with 26.8psi vacuum @15:00:34.40 Intake.close @15:00:34.51 Exhaust.close @15:00:35.26 Sampled 250.0ml @15:00:35.38 TV.seek :bypass @15:00:37.03 TV.seek :clear @15:00:40.52 SPR.command "read_sequences" @15:00:40.62 SPR.command "stats_on" @15:00:40.68 SPR.command "start 2 0" @15:00:42.68 Begun log /var/log/esp/spr18jul2515h.spr @15:00:43.48 Handoff.select :waste @15:00:43.63 ArS.seek empty @15:01:01.37 PinPort.seek :cartridge @15:01:07.13 LyS1.seek 1350ul @15:01:13.10 PV.seek :reagent @15:01:13.96 AirS.seek :tVent @15:01:16.07 delay 2 seconds @15:01:18.11 AirS.reconfigure EvacAirS @15:01:18.19 AirS.seek :evac @15:01:40.53 delay 15 seconds @15:01:55.56 AirS.reconfigure AirSconfig @15:01:55.65 AirS.seek :tVent @15:02:09.89 delay 2 seconds @15:02:11.93 AirS.reconfigure EvacAirS @15:02:12.02 AirS.seek :evac @15:02:34.35 delay 15 seconds @15:02:49.38 AirS.reconfigure AirSconfig @15:02:49.47 AirS.seek :tVent @15:03:03.71 delay 2 seconds @15:03:05.74 AirS.reconfigure EvacAirS @15:03:05.85 AirS.seek :evac @15:03:28.19 delay 15 seconds @15:03:43.22 AirS.reconfigure AirSconfig @15:03:43.32 AirS.seek :tVent @15:03:56.44 SPR.command "start 1 14" @15:03:57.86 Ended log /var/log/esp/spr18jul2515h.spr @15:03:57.88 SPR.command "stats_off" @15:03:58.82 Cmd::Abort in PROCESSING -- Storage Carousel positionErr at 1 (actually 1) during Air Syringe move @15:03:58.87 Slide::Error in MAIN -- Storage Carousel positionErr at 1 (actually 1) during Air Syringe move @15:03:58.92 Thread::ChildDied in MAIN_scheduler -- :MAIN @15:03:59.03 Handoff.select :close @15:03:59.24 Session Ended @12:43:15.37PDT26-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @12:43:15.40 >BREAK< ESP3 Gateway v0.99 for cartridge bus @12:43:16.98 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @12:43:18.44 Polling Can every 5:00 @12:43:18.54 Gate.power :analytic1,:ON,limit to 5000mA @12:43:18.56 Gate.power :analytic2,:ON,limit to 5000mA @12:43:18.57 Gate.power :analytic3,:ON,limit to 5000mA @12:43:19.26 ArS.configure ArSconfig @12:43:19.37 LyS1.configure LyS1config @12:43:19.51 LyS2.configure LyS2config @12:43:19.62 AirS.configure AirSconfig @12:43:19.75 TV.configure TVconfig @12:43:19.91 SP.configure SPconfig @12:43:19.98 SPR Module 1, FW Rev. B, FPGA Rev. C @12:43:20.09
SC.reconfigure SCconfig @12:43:20.14 SC.hold @12:43:20.21 SC.configure SCconfig @12:43:20.34 PV.configure PVconfig @12:43:20.49 PinPort.configure PPconfig @12:43:20.63 Sea.configure SPconfig @12:43:20.70 All elf controllers running firmware version 4.46 @12:43:20.79 Intake.close @12:43:20.85 Exhaust.close @12:43:20.92 PB.close @12:43:21.11
Loading /home/esp/esp2/mission/processAll.rb mission @12:43:21.14 Processing all remaining dry cartridges in: {dry: [12, 14, 16, 18, 20, 22, 24]} @12:43:21.15 Sampling maximum of 500.0ml per cartridge @12:43:21.17 Fixing samples for 10:00, evacuating once @12:43:21.19 delay 30 seconds @12:43:51.96 SC.seek 8 @12:43:52.04 Selecting Cartridge 24 @12:43:52.40 SC.seek 24 @12:43:55.98 LyS2.seek :locked @12:43:56.14 PV.seek :locked @12:43:56.30 AirS.seek :locked @12:44:00.38
Powering cartridge bus @12:44:02.51 Gate.setIR 1750,5000,0,6 @12:44:02.63 Gate.setIR 0,5000,0,6 @12:44:02.70 Loaded Lyse-n-Go Cartridge @12:44:02.71 PuckHeater.configure HeatConfig @12:44:02.76 Gate.power :heat,:ON,limit to 5000mA @12:44:03.06 Duration of filtering limited to 1:05:00 @12:44:03.40 Priming sample loop w/100ml, bypass w/10ml @12:44:03.49 SP.setPosition! 0ml @12:44:03.54 Intake.open @12:44:03.60 Exhaust.open @12:44:04.42 Sea @-42.9psia @12:44:04.44 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:44:10.17 Vacuum tare is 21% of full scale @12:44:10.19 PB.open @12:44:10.22 Exhaust.close @12:44:11.46 Sea @-42.9psia @12:44:11.56 SP.reconfigure SPprime @12:44:11.63 SP.seek 10ml,1:21 @12:44:12.97 Session Begun @12:44:16.79 -> showlog @12:44:22.51 SP.setPosition! 0ml @12:44:22.60 PB.close @12:44:22.64 Exhaust.open @12:44:23.53 Sea @-42.9psia @12:44:23.66 SP.reconfigure SPprime @12:44:23.76 SP.seek 100ml,8:51 @12:46:10.88 Pressurizing cartridge to 26psi @12:46:10.89 Intake.close @12:46:11.69 SP.setPosition! 0ml @12:46:11.75 PB.open @12:46:12.59 TV.seek :crack @12:46:13.42 TV.seek :clear @12:46:14.25 Intake.open @12:46:15.71 PB.close @12:46:16.44 Exhaust.close @12:46:17.26 Sea @-42.9psia @12:46:17.39 SP.reconfigure SPcharge @12:46:17.48 SP.seek 7.5ml,1:08 @12:46:23.50 Cartridge pressurized with 0.164ml to 26.0psi @12:46:23.78 delay 3 seconds @12:46:26.89 Cartridge at 12.4psi 3 seconds later @12:46:27.04 TV.seek :cartridge @12:46:28.89 TV.seek :clear @12:46:30.73 Sampling 500.0ml @12:46:30.75 SP.setPosition! 0ml @12:46:30.84 Exhaust.open @12:46:31.69 Sea @-42.9psia @12:46:31.82 SP.reconfigure SPsample @12:46:31.92 SP.seek 500ml,42:12 @12:46:32.85 Cartridge 24 sampling 0/500ml at 0.15ml/s with 1.9psi vacuum @12:47:32.85 Cartridge 24 sampling 35/500ml at 0.61ml/s with 22.0psi vacuum @12:48:32.85 Cartridge 24 sampling 70/500ml at 0.60ml/s with 25.1psi vacuum @12:49:32.86 Cartridge 24 sampling 105/500ml at 0.56ml/s with 25.0psi vacuum @12:50:32.85 Cartridge 24 sampling 140/500ml at 0.56ml/s with 18.9psi vacuum @12:51:32.86 Cartridge 24 sampling 175/500ml at 0.58ml/s with 21.4psi vacuum @12:52:32.86 Cartridge 24 sampling 209/500ml at 0.57ml/s with 26.2psi vacuum @12:53:32.85 Cartridge 24 sampling 243/500ml at 0.56ml/s with 16.8psi vacuum @12:54:32.85 Cartridge 24 sampling 277/500ml at 0.56ml/s with 17.7psi vacuum @12:55:32.85 Cartridge 24 sampling 310/500ml at 0.59ml/s with 23.6psi vacuum @12:56:32.85 Cartridge 24 sampling 344/500ml at 0.53ml/s with 15.0psi vacuum @12:57:32.86 Cartridge 24 sampling 377/500ml at 0.55ml/s with 28.0psi vacuum @12:58:32.85 Cartridge 24 sampling 410/500ml at 0.54ml/s with 17.0psi vacuum @12:59:32.86 Cartridge 24 sampling 443/500ml at 0.53ml/s with 28.8psi vacuum @13:00:32.85 Cartridge 24 sampling 475/500ml at 0.56ml/s with 15.3psi vacuum @13:01:24.01 Intake.close @13:01:24.07 Exhaust.close @13:01:24.94 Sampled 500.0ml @13:01:25.06 TV.seek :bypass @13:01:26.71 TV.seek :clear @13:01:30.23 SPR.command "read_sequences" @13:01:30.41 SPR.command "stats_on" @13:01:30.47 SPR.command "start 2 0" @13:01:32.48 Begun log /var/log/esp/spr18jul2613h.spr @13:01:33.25 Handoff.select :waste @13:01:33.42 ArS.seek empty @13:01:51.15 PinPort.seek :cartridge @13:01:56.89 LyS1.seek 1350ul @13:02:02.86 PV.seek :reagent @13:02:03.74 AirS.seek :tVent @13:02:05.84 delay 2 seconds @13:02:07.87 AirS.reconfigure EvacAirS @13:02:07.96 AirS.seek :evac @13:02:30.29 delay 15 seconds @13:02:45.32 AirS.reconfigure AirSconfig @13:02:45.41 AirS.seek :tVent @13:02:59.64 delay 2 seconds @13:03:01.67 AirS.reconfigure EvacAirS @13:03:01.79 AirS.seek :evac @13:03:24.10 delay 15 seconds @13:03:39.13 AirS.reconfigure AirSconfig @13:03:39.24 AirS.seek :tVent @13:03:53.47 delay 2 seconds @13:03:55.50 AirS.reconfigure EvacAirS @13:03:55.59 AirS.seek :evac @13:04:17.93 delay 15 seconds @13:04:32.96 AirS.reconfigure AirSconfig @13:04:33.05 AirS.seek :tVent @13:04:47.29 delay 2 seconds @13:04:49.32 AirS.reconfigure EvacAirS @13:04:49.40 AirS.seek :evac @13:05:11.73 delay 15 seconds @13:05:26.76 delay 1:30 @13:06:27.11 -> evacSW 1 @13:06:27.16 AirS.reconfigure AirSconfig @13:06:27.27 AirS.seek :tVent @13:06:41.57 delay 2 seconds @13:06:43.60 AirS.reconfigure EvacAirS @13:06:43.68 AirS.seek :evac @13:06:56.78 AirS.seek :fVent @13:06:56.93 Slide::Error in bill -- Air Syringe aborted at 810ul (actually 816ul) @13:07:07.54 delay 15 seconds @13:07:22.58 LyS1.reconfigure SlowLyS1 @13:07:22.74 LyS1.seek 1050ul @13:07:52.80 delay 5 seconds @13:07:57.82 Handoff.select :blocked @13:07:58.06 LyS1.seek 450ul @13:08:58.04 delay 10 seconds @13:09:08.08 AirS.reconfigure SlowAirS @13:09:08.23 AirS.reconfigure to mix into 1.37806 parts LyS1 @13:09:08.76 AirS.seek evac @13:09:08.81 LyS1.seek 300ul @13:09:23.98 AirS.reconfigure AirSconfig @13:09:24.07 AirS.seek :full @13:09:38.31 LyS1.seek :empty @13:10:08.43 PuckHeater.seek 84.3C @13:11:12.19 PuckHeater.hold 15:00,84.3C @13:26:12.13 LyS1.reconfigure LyS1config @13:26:12.24 LyS1.seek 900ul @13:26:20.62 AirS.seek :tVent @13:26:20.75 delay 2 seconds @13:26:22.78 AirS.reconfigure HalfSpeedAirS @13:26:22.88 AirS.seek :evac @13:26:50.64 delay 15 seconds @13:27:05.67 AirS.reconfigure AirSconfig @13:27:05.83 AirS.seek :tVent @13:27:20.08 delay 2 seconds @13:27:22.11 AirS.reconfigure HalfSpeedAirS @13:27:22.20 AirS.seek :evac @13:27:49.97 delay 15 seconds @13:28:05.00 AirS.reconfigure AirSconfig @13:28:05.09 AirS.seek :tVent @13:28:19.34 delay 2 seconds @13:28:21.37 AirS.reconfigure HalfSpeedAirS @13:28:21.46 AirS.seek :evac @13:28:49.23 delay 15 seconds @13:29:04.26 delay 1:00 @13:30:04.27 Handoff.select :waste @13:30:04.40 delay 15 seconds @13:30:19.43 AirS.seek :fVent @13:30:21.66 delay 10 seconds @13:30:31.69 AirS.reconfigure AirSconfig @13:30:31.78 AirS.seek :evac @13:30:33.24 Lysis Recovered in LyS1 @13:30:33.26 Lysis Complete @13:30:33.28 Handoff.select :waste @13:30:33.35 LyS2.seek :full @13:30:33.46 LyS1.reconfigure DAmixLyS1 @13:30:33.54 LyS2.reconfigure DAmixLyS2 @13:30:34.09 LyS1.seek 650ul @13:30:34.17 LyS2.seek 1480ul @13:31:21.49 delay 5 seconds @13:31:26.52 Handoff.select :SPR @13:31:26.66 SPR.command "start 1 14" @13:31:27.49 Running Experiment-Rep #1 @13:31:27.50 delay 10 seconds @13:31:37.53 SPR.command "start 1 0" @13:31:40.86 Waiting up to 4:00 for SPR to enter state 1 @13:33:45.13 SPR.command "hold_on" @13:33:47.50 LyS1.seek 300ul @13:33:47.55 LyS2.seek 1130ul @13:34:53.81 delay 15 seconds @13:35:08.83 SPR.command "hold_off" @13:35:11.77 Waiting up to 25:00 for SPR to enter state 7 @13:55:15.24 Handoff.select :waste @13:55:15.67 LyS1.reconfigure LyS1config @13:55:15.75 LyS2.reconfigure LyS2config @13:55:15.83 Rep #1 SPR Analysis has Completed @13:55:16.16 Ended log /var/log/esp/spr18jul2613h.spr @13:55:16.18 SPR.command "stats_off" @13:55:16.70 Handoff.select :close @13:55:16.94
PV.seek :locked @13:55:17.96 ArS.seek :clear @13:55:18.14 LyS1.seek :clear @13:55:18.33 LyS2.seek :clear @13:55:18.55 AirS.seek :clear @13:55:35.77
ArS.coast @13:55:35.85 LyS1.coast @13:55:35.92 LyS2.coast @13:55:36.00 AirS.coast @13:55:36.07 TV.coast @13:55:36.15 SP.coast @13:55:36.22 SC.coast @13:55:36.30 PV.coast @13:55:36.37 PinPort.coast @13:55:36.61 Gate.power :motor,:OFF @13:55:36.63 Gate.power :heat,:OFF @13:55:36.67 Gate.power :main,:OFF @13:55:36.70 Gate.power :cartridge,:OFF @13:55:36.74 Gate.power :core,:OFF @13:55:36.77 Gate.power :analytic1,:OFF @13:55:36.80 Gate.power :analytic2,:OFF @13:55:36.83 Gate.power :analytic3,:OFF @13:55:36.86 Safely stopped and ready to power off @13:55:37.07 delay 30 seconds @13:56:07.20 Gate.power :main,:ON,limit to 5000mA @13:56:07.23 Gate.power :analytic1,:ON,limit to 5000mA @13:56:07.28 Gate.power :analytic2,:ON,limit to 5000mA @13:56:07.32 Gate.power :analytic3,:ON,limit to 5000mA @13:56:07.42 Powering core bus @13:56:09.80 SPR Module 1, FW Rev. B, FPGA Rev. C @13:56:09.98
ArS.configure ArSconfig @13:56:10.13 LyS1.configure LyS1config @13:56:10.30 LyS2.configure LyS2config @13:56:10.43 AirS.configure AirSconfig @13:56:10.61 TV.configure TVconfig @13:56:10.78 SP.configure SPconfig @13:56:10.94 SC.configure SCconfig @13:56:11.12 PV.configure PVconfig @13:56:11.31 PinPort.configure PPconfig @13:56:11.47 Sea.configure SPconfig @13:56:11.55 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @13:56:11.58 Gate.power :motor,:ON,limit to 5000mA @13:56:11.77 ArS.reconfigure ArSconfig @13:56:12.13 ArS.home.jog 0 @13:56:12.48 PinPort.reconfigure PPconfig @13:56:12.57 PV.reconfigure PVconfig @13:56:12.63 TV.reconfigure TVconfig @13:56:12.69 LyS1.reconfigure LyS1config @13:56:12.75 LyS2.reconfigure LyS2config @13:56:12.87 AirS.reconfigure AirSconfig @13:56:13.14 PinPort.home.jog 0 @13:56:13.19 PV.home.jog 0 @13:56:13.22 TV.home.jog 0 @13:56:13.26 LyS1.home.jog 0 @13:56:13.31 LyS2.home.jog 0 @13:56:13.36 AirS.home.jog 0 @13:56:13.94 ArS.reconfigure HomeMagSyringe @13:56:14.79 LyS2.reconfigure HomeSyringe @13:56:14.87 ArS.jog -277 @13:56:14.92 PV.reconfigure HomePV @13:56:14.97 AirS.reconfigure HomeMagSyringe @13:56:15.03 PinPort.reconfigure HomePP @13:56:15.10 TV.reconfigure HomeTV @13:56:15.17 LyS1.reconfigure HomeSyringe @13:56:15.48 LyS2.jog -265 @13:56:15.54 PV.jog -1505 @13:56:15.60 PinPort.jog -2410 @13:56:15.66 TV.jog -548 @13:56:15.70 AirS.jog -312 @13:56:15.79 LyS1.jog -274 @13:56:16.11 ArS.setRawPosition! -50 @13:56:16.19 ArS.seek :home @13:56:16.31 LyS2.setRawPosition! -50 @13:56:16.40 LyS2.seek :home @13:56:16.53 ArS.reconfigure ArSconfig @13:56:16.67 AirS.setRawPosition! -50 @13:56:16.71 LyS1.setRawPosition! -50 @13:56:16.83 AirS.seek :home @13:56:16.90 LyS1.seek :home @13:56:17.10 PV.setRawPosition! -50 @13:56:17.13 LyS2.reconfigure LyS2config @13:56:17.29 PV.seek :home @13:56:17.47 LyS1.reconfigure LyS1config @13:56:17.50 AirS.reconfigure AirSconfig @13:56:17.70 PV.reconfigure PVconfig @13:56:17.86 TV.setRawPosition! 0 @13:56:17.93 TV.seek :home @13:56:18.07 TV.reconfigure TVconfig @13:56:21.65 PinPort.setRawPosition! 0 @13:56:21.73 PinPort.seek :home @13:56:21.85 PinPort.reconfigure PPconfig @13:56:22.42
TV.seek :clear @13:56:24.05 TV.coast @13:56:24.13 SC.reconfigure HomeSC @13:56:24.22 SC.home.jog 0 @13:56:25.03 SC.reconfigure SCconfig @13:56:25.11 SC.seek 24 @13:56:25.26 Selecting Cartridge 22 @13:56:25.65 SC.seek 22 @13:56:26.43 LyS2.seek :locked @13:56:26.62 PV.seek :locked @13:56:26.81 AirS.seek :locked @13:56:30.93
Powering cartridge bus @13:56:33.07 Gate.setIR 1750,5000,0,6 @13:56:33.19 Gate.setIR 0,5000,0,6 @13:56:33.27 Loaded Lyse-n-Go Cartridge @13:56:33.28 PuckHeater.configure HeatConfig @13:56:33.38 Gate.power :heat,:ON,limit to 5000mA @13:56:33.69 Duration of filtering limited to 1:05:00 @13:56:34.36 Priming sample loop w/100ml, bypass w/10ml @13:56:34.48 SP.setPosition! 0ml @13:56:34.55 Intake.open @13:56:34.64 Exhaust.open @13:56:35.44 Sea @-42.9psia @13:56:35.46 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @13:56:41.22 Vacuum tare is 20% of full scale @13:56:41.24 PB.open @13:56:41.30 Exhaust.close @13:56:42.15 Sea @-42.9psia @13:56:42.28 SP.reconfigure SPprime @13:56:42.38 SP.seek 10ml,1:21 @13:56:53.26 SP.setPosition! 0ml @13:56:53.34 PB.close @13:56:53.38 Exhaust.open @13:56:54.25 Sea @-42.9psia @13:56:54.37 SP.reconfigure SPprime @13:56:54.47 SP.seek 100ml,8:51 @13:58:40.80 Pressurizing cartridge to 26psi @13:58:40.82 Intake.close @13:58:41.62 SP.setPosition! 0ml @13:58:41.69 PB.open @13:58:42.51 TV.seek :crack @13:58:43.34 TV.seek :clear @13:58:44.17 Intake.open @13:58:45.62 PB.close @13:58:46.38 Exhaust.close @13:58:47.18 Sea @-42.9psia @13:58:47.30 SP.reconfigure SPcharge @13:58:47.40 SP.seek 7.5ml,1:08 @13:59:01.21 Cartridge pressurized with 0.893ml to 26.3psi @13:59:01.51 delay 3 seconds @13:59:04.60 Cartridge at 24.2psi 3 seconds later @13:59:04.72 TV.seek :cartridge @13:59:06.56 TV.seek :clear @13:59:08.41 Sampling 500.0ml @13:59:08.42 SP.setPosition! 0ml @13:59:08.51 Exhaust.open @13:59:09.29 Sea @-42.9psia @13:59:09.42 SP.reconfigure SPsample @13:59:09.52 SP.seek 500ml,42:12 @13:59:10.45 Cartridge 22 sampling 0/500ml at 0.15ml/s with 4.0psi vacuum @14:00:10.45 Cartridge 22 sampling 36/500ml at 0.59ml/s with 23.4psi vacuum @14:01:10.47 Cartridge 22 sampling 72/500ml at 0.59ml/s with 22.4psi vacuum @14:02:10.46 Cartridge 22 sampling 108/500ml at 0.61ml/s with 24.9psi vacuum @14:03:10.45 Cartridge 22 sampling 143/500ml at 0.59ml/s with 23.9psi vacuum @14:04:10.45 Cartridge 22 sampling 179/500ml at 0.59ml/s with 22.8psi vacuum @14:05:10.46 Cartridge 22 sampling 214/500ml at 0.56ml/s with 23.6psi vacuum @14:06:10.46 Cartridge 22 sampling 248/500ml at 0.58ml/s with 19.0psi vacuum @14:07:10.81 Cartridge 22 sampling 283/500ml at 0.57ml/s with 21.5psi vacuum @14:08:10.45 Cartridge 22 sampling 317/500ml at 0.58ml/s with 25.3psi vacuum @14:09:10.46 Cartridge 22 sampling 351/500ml at 0.58ml/s with 21.0psi vacuum @14:10:10.47 Cartridge 22 sampling 386/500ml at 0.58ml/s with 23.7psi vacuum @14:11:10.46 Cartridge 22 sampling 420/500ml at 0.58ml/s with 23.1psi vacuum @14:12:10.46 Cartridge 22 sampling 454/500ml at 0.58ml/s with 19.7psi vacuum @14:13:10.45 Cartridge 22 sampling 488/500ml at 0.55ml/s with 25.2psi vacuum @14:13:31.91 Intake.close @14:13:31.98 Exhaust.close @14:13:32.78 Sampled 500.0ml @14:13:32.90 TV.seek :bypass @14:13:34.53 TV.seek :clear @14:13:38.02 SPR.command "read_sequences" @14:13:38.61 SPR.command "stats_on" @14:13:38.67 SPR.command "start 2 0" @14:13:40.68 Begun log /var/log/esp/spr18jul2614h.spr @14:13:41.47 Handoff.select :waste @14:13:41.62 ArS.seek empty @14:13:59.37 PinPort.seek :cartridge @14:14:05.12 LyS1.seek 1350ul @14:14:11.09 PV.seek :reagent @14:14:11.97 AirS.seek :tVent @14:14:14.07 delay 2 seconds @14:14:16.10 AirS.reconfigure EvacAirS @14:14:16.19 AirS.seek :evac @14:14:38.51 delay 15 seconds @14:14:53.55 AirS.reconfigure AirSconfig @14:14:53.63 AirS.seek :tVent @14:15:07.87 delay 2 seconds @14:15:09.92 AirS.reconfigure EvacAirS @14:15:10.01 AirS.seek :evac @14:15:32.33 delay 15 seconds @14:15:47.36 AirS.reconfigure AirSconfig @14:15:47.45 AirS.seek :tVent @14:16:01.69 delay 2 seconds @14:16:03.72 AirS.reconfigure EvacAirS @14:16:03.81 AirS.seek :evac @14:16:26.17 delay 15 seconds @14:16:41.20 AirS.reconfigure AirSconfig @14:16:41.29 AirS.seek :tVent @14:16:55.54 delay 2 seconds @14:16:57.57 AirS.reconfigure EvacAirS @14:16:57.65 AirS.seek :evac @14:17:20.00 delay 15 seconds @14:17:35.03 delay 1:30 @14:17:41.29 -> evacSW 1 @14:17:41.33 AirS.reconfigure AirSconfig @14:17:41.43 AirS.seek :tVent @14:17:55.68 delay 2 seconds @14:17:57.71 AirS.reconfigure EvacAirS @14:17:57.80 AirS.seek :evac @14:18:20.12 delay 15 seconds @14:19:05.06 AirS.reconfigure AirSconfig @14:19:05.16 AirS.seek :fVent @14:19:06.62 delay 15 seconds @14:19:21.65 LyS1.reconfigure SlowLyS1 @14:19:21.79 LyS1.seek 1050ul @14:19:51.83 delay 5 seconds @14:19:56.85 Handoff.select :blocked @14:19:57.07 LyS1.seek 450ul @14:20:57.04 delay 10 seconds @14:21:07.08 AirS.reconfigure SlowAirS @14:21:07.22 AirS.reconfigure to mix into 1.37806 parts LyS1 @14:21:07.82 AirS.seek evac @14:21:07.85 LyS1.seek 300ul @14:21:23.02 AirS.reconfigure AirSconfig @14:21:23.11 AirS.seek :full @14:21:37.34 LyS1.seek :empty @14:22:07.44 PuckHeater.seek 84.3C @14:23:08.80 PuckHeater.hold 15:00,84.3C @14:38:05.30 LyS1.reconfigure LyS1config @14:38:05.41 LyS1.seek 900ul @14:38:13.77 AirS.seek :tVent @14:38:13.90 delay 2 seconds @14:38:15.93 AirS.reconfigure HalfSpeedAirS @14:38:16.02 AirS.seek :evac @14:38:44.13 delay 15 seconds @14:38:59.18 AirS.reconfigure AirSconfig @14:38:59.27 AirS.seek :tVent @14:39:13.51 delay 2 seconds @14:39:15.54 AirS.reconfigure HalfSpeedAirS @14:39:15.63 AirS.seek :evac @14:39:43.41 delay 15 seconds @14:39:58.45 AirS.reconfigure AirSconfig @14:39:58.53 AirS.seek :tVent @14:40:12.77 delay 2 seconds @14:40:14.80 AirS.reconfigure HalfSpeedAirS @14:40:14.88 AirS.seek :evac @14:40:42.69 delay 15 seconds @14:40:57.72 delay 1:00 @14:41:57.74 Handoff.select :waste @14:41:57.91 delay 15 seconds @14:42:12.94 AirS.seek :fVent @14:42:15.19 delay 10 seconds @14:42:25.23 AirS.reconfigure AirSconfig @14:42:25.34 AirS.seek :evac @14:42:26.82 Lysis Recovered in LyS1 @14:42:26.84 Lysis Complete @14:42:26.87 Handoff.select :waste @14:42:26.93 LyS2.seek :full @14:42:27.05 LyS1.reconfigure DAmixLyS1 @14:42:27.17 LyS2.reconfigure DAmixLyS2 @14:42:27.72 LyS1.seek 650ul @14:42:27.76 LyS2.seek 1480ul @14:43:15.16 delay 5 seconds @14:43:20.18 Handoff.select :SPR @14:43:20.32 SPR.command "start 1 14" @14:43:21.51 Running Experiment-Rep #1 @14:43:21.53 delay 10 seconds @14:43:31.56 SPR.command "start 1 0" @14:43:34.87 Waiting up to 4:00 for SPR to enter state 1 @14:45:39.16 SPR.command "hold_on" @14:45:40.99 LyS1.seek 300ul @14:45:41.08 LyS2.seek 1130ul @14:46:47.26 delay 15 seconds @14:47:02.28 SPR.command "hold_off" @14:47:04.86 Waiting up to 25:00 for SPR to enter state 7 @15:07:09.33 Handoff.select :waste @15:07:09.48 LyS1.reconfigure LyS1config @15:07:09.56 LyS2.reconfigure LyS2config @15:07:09.64 Rep #1 SPR Analysis has Completed @15:07:10.18 Ended log /var/log/esp/spr18jul2614h.spr @15:07:10.20 SPR.command "stats_off" @15:07:10.73 Handoff.select :close @15:07:10.97
PV.seek :locked @15:07:12.02 ArS.seek :clear @15:07:12.22 LyS1.seek :clear @15:07:12.42 LyS2.seek :clear @15:07:12.62 AirS.seek :clear @15:07:29.40 -> abort! 'reset' @15:07:29.43 CmdServer::Client::Error in bill -- Aborting Mission: reset @15:07:29.49 SystemExit in bill -- reset @15:07:29.52
ArS.stop @15:07:29.78 Session Ended @15:09:49.30PDT26-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @15:09:49.33 >BREAK< ESP3 Gateway v0.99 for cartridge bus @15:09:50.85 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @15:09:52.40 Polling Can every 5:00 @15:09:52.52 Gate.power :analytic1,:ON,limit to 5000mA @15:09:52.53 Gate.power :analytic2,:ON,limit to 5000mA @15:09:52.55 Gate.power :analytic3,:ON,limit to 5000mA @15:09:53.46 ArS.configure ArSconfig @15:09:53.60 LyS1.configure LyS1config @15:09:53.73 LyS2.configure LyS2config @15:09:53.84 AirS.configure AirSconfig @15:09:53.99 TV.configure TVconfig @15:09:54.15 SP.configure SPconfig @15:09:54.31 SPR Module 1, FW Rev. B, FPGA Rev. C @15:09:54.33
SC.reconfigure SCconfig @15:09:54.39 SC.hold @15:09:54.45 SC.configure SCconfig @15:09:54.58 PV.configure PVconfig @15:09:54.72 PinPort.configure PPconfig @15:09:54.79 PuckHeater.coast @15:09:54.85 PuckHeater.configure HeatConfig @15:09:54.99 Sea.configure SPconfig @15:09:55.05 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @15:09:55.13 Intake.close @15:09:55.17 Exhaust.close @15:09:55.29 PB.close @15:09:55.44
Loading /home/esp/esp2/mission/processAll.rb mission @15:09:55.48 Processing all remaining dry cartridges in: {500 => [22, 24], dry: [16, 18, 20]} @15:09:55.49 Sampling maximum of 500.0ml per cartridge @15:09:55.50 Fixing samples for 10:00, evacuating once @15:09:55.52 delay 30 seconds @15:10:13.05 Session Begun @15:10:26.27
SC.seek 22 @15:10:26.38 Selecting Cartridge 20 @15:10:26.62 Gate.power :heat,:OFF @15:10:26.64 Gate.power :cartridge,:OFF @15:10:26.80 ArS.seek :clear @15:10:27.24 PinPort.seek :clear @15:10:33.14 PV.seek :clear @15:10:34.27
SC.seek 20 @15:10:35.04 LyS2.seek :locked @15:10:35.25 PV.seek :locked @15:10:35.43 AirS.seek :locked @15:10:39.54
Powering cartridge bus @15:10:41.67 Gate.setIR 1750,5000,0,6 @15:10:41.80 Gate.setIR 0,5000,0,6 @15:10:41.88 Loaded Lyse-n-Go Cartridge @15:10:41.89 PuckHeater.configure HeatConfig @15:10:41.98 Gate.power :heat,:ON,limit to 5000mA @15:10:42.28 Duration of filtering limited to 1:05:00 @15:10:42.63 Priming sample loop w/100ml, bypass w/10ml @15:10:42.74 SP.setPosition! 0ml @15:10:42.81 Intake.open @15:10:42.84 Exhaust.open @15:10:44.10 Sea @-42.9psia @15:10:44.11 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @15:10:49.88 Vacuum tare is 21% of full scale @15:10:49.90 PB.open @15:10:50.01 Exhaust.close @15:10:50.83 Sea @-42.9psia @15:10:50.95 SP.reconfigure SPprime @15:10:51.04 SP.seek 10ml,1:21 @15:11:01.97 SP.setPosition! 0ml @15:11:02.05 PB.close @15:11:02.11 Exhaust.open @15:11:02.96 Sea @-42.9psia @15:11:03.09 SP.reconfigure SPprime @15:11:03.18 SP.seek 100ml,8:51 @15:12:49.27 Pressurizing cartridge to 26psi @15:12:49.28 Intake.close @15:12:50.10 SP.setPosition! 0ml @15:12:50.18 PB.open @15:12:51.03 TV.seek :crack @15:12:51.87 TV.seek :clear @15:12:52.69 Intake.open @15:12:54.16 PB.close @15:12:54.90 Exhaust.close @15:12:55.70 Sea @-42.9psia @15:12:55.82 SP.reconfigure SPcharge @15:12:55.92 SP.seek 7.5ml,1:08 @15:13:02.68 Cartridge pressurized with 0.207ml to 26.1psi @15:13:02.98 delay 3 seconds @15:13:06.07 Cartridge at 18.6psi 3 seconds later @15:13:06.20 TV.seek :cartridge @15:13:08.07 TV.seek :clear @15:13:09.92 Sampling 500.0ml @15:13:09.93 SP.setPosition! 0ml @15:13:10.02 Exhaust.open @15:13:10.78 Sea @-42.9psia @15:13:10.92 SP.reconfigure SPsample @15:13:11.01 SP.seek 500ml,42:12 @15:13:11.95 Cartridge 20 sampling 0/500ml at 0.15ml/s with 2.0psi vacuum @15:14:11.94 Cartridge 20 sampling 36/500ml at 0.61ml/s with 21.0psi vacuum @15:15:11.95 Cartridge 20 sampling 72/500ml at 0.60ml/s with 18.5psi vacuum @15:16:11.95 Cartridge 20 sampling 107/500ml at 0.61ml/s with 22.8psi vacuum @15:17:11.95 Cartridge 20 sampling 142/500ml at 0.60ml/s with 24.9psi vacuum @15:18:11.94 Cartridge 20 sampling 177/500ml at 0.60ml/s with 25.6psi vacuum @15:19:11.94 Cartridge 20 sampling 211/500ml at 0.59ml/s with 25.7psi vacuum @15:20:11.93 Cartridge 20 sampling 246/500ml at 0.60ml/s with 23.8psi vacuum @15:21:11.95 Cartridge 20 sampling 279/500ml at 0.57ml/s with 26.9psi vacuum @15:22:11.95 Cartridge 20 sampling 313/500ml at 0.54ml/s with 15.8psi vacuum @15:23:11.95 Cartridge 20 sampling 346/500ml at 0.56ml/s with 18.4psi vacuum @15:24:11.96 Cartridge 20 sampling 379/500ml at 0.52ml/s with 24.8psi vacuum @15:25:12.30 Cartridge 20 sampling 412/500ml at 0.52ml/s with 25.2psi vacuum @15:26:11.95 Cartridge 20 sampling 444/500ml at 0.56ml/s with 22.1psi vacuum @15:27:11.95 Cartridge 20 sampling 476/500ml at 0.56ml/s with 23.9psi vacuum @15:27:57.16 Intake.close @15:27:57.20 Exhaust.close @15:27:58.06 Sampled 500.0ml @15:27:58.19 TV.seek :bypass @15:27:59.85 TV.seek :clear @15:28:03.34 SPR.command "read_sequences" @15:28:03.84 SPR.command "stats_on" @15:28:03.91 SPR.command "start 2 0" @15:28:05.92 Begun log /var/log/esp/spr18jul2615h.spr @15:28:06.69 Handoff.select :waste @15:28:06.86 ArS.seek empty @15:28:24.60 PinPort.seek :cartridge @15:28:30.37 LyS1.seek 1350ul @15:28:36.34 PV.seek :reagent @15:28:37.19 AirS.seek :tVent @15:28:39.32 delay 2 seconds @15:28:41.36 AirS.reconfigure EvacAirS @15:28:41.45 AirS.seek :evac @15:29:03.76 delay 15 seconds @15:29:18.79 AirS.reconfigure AirSconfig @15:29:18.88 AirS.seek :tVent @15:29:33.14 delay 2 seconds @15:29:35.17 AirS.reconfigure EvacAirS @15:29:35.26 AirS.seek :evac @15:29:57.57 delay 15 seconds @15:30:12.60 AirS.reconfigure AirSconfig @15:30:12.68 AirS.seek :tVent @15:30:26.92 delay 2 seconds @15:30:28.95 AirS.reconfigure EvacAirS @15:30:29.04 AirS.seek :evac @15:30:51.37 delay 15 seconds @15:31:06.40 AirS.reconfigure AirSconfig @15:31:06.49 AirS.seek :tVent @15:31:20.74 delay 2 seconds @15:31:22.77 AirS.reconfigure EvacAirS @15:31:22.86 AirS.seek :evac @15:31:45.16 delay 15 seconds @15:32:00.19 AirS.reconfigure AirSconfig @15:32:00.30 AirS.seek :tVent @15:32:14.53 delay 2 seconds @15:32:16.57 AirS.reconfigure EvacAirS @15:32:16.65 AirS.seek :evac @15:32:38.99 delay 15 seconds @15:32:54.02 delay 1:30 @15:34:24.04 AirS.reconfigure AirSconfig @15:34:24.13 AirS.seek :fVent @15:34:25.60 delay 15 seconds @15:34:40.64 LyS1.reconfigure SlowLyS1 @15:34:40.80 LyS1.seek 1050ul @15:35:10.84 delay 5 seconds @15:35:15.86 Handoff.select :blocked @15:35:16.07 LyS1.seek 450ul @15:36:16.05 delay 10 seconds @15:36:26.08 AirS.reconfigure SlowAirS @15:36:26.23 AirS.reconfigure to mix into 1.37806 parts LyS1 @15:36:26.79 AirS.seek evac @15:36:26.83 LyS1.seek 300ul @15:36:42.02 AirS.reconfigure AirSconfig @15:36:42.11 AirS.seek :full @15:36:56.36 LyS1.seek :empty @15:37:26.46 PuckHeater.seek 84.2C @15:38:27.50 PuckHeater.hold 15:00,84.2C @15:53:26.05 LyS1.reconfigure LyS1config @15:53:26.15 LyS1.seek 900ul @15:53:34.56 AirS.seek :tVent @15:53:34.70 delay 2 seconds @15:53:36.73 AirS.reconfigure HalfSpeedAirS @15:53:36.81 AirS.seek :evac @15:54:04.58 delay 15 seconds @15:54:19.61 AirS.reconfigure AirSconfig @15:54:19.97 AirS.seek :tVent @15:54:34.31 delay 2 seconds @15:54:36.34 AirS.reconfigure HalfSpeedAirS @15:54:36.42 AirS.seek :evac @15:55:04.19 delay 15 seconds @15:55:19.22 AirS.reconfigure AirSconfig @15:55:19.32 AirS.seek :tVent @15:55:33.55 delay 2 seconds @15:55:35.58 AirS.reconfigure HalfSpeedAirS @15:55:35.67 AirS.seek :evac @15:56:03.45 delay 15 seconds @15:56:18.48 delay 1:00 @15:57:18.50 Handoff.select :waste @15:57:18.65 delay 15 seconds @15:57:33.68 AirS.seek :fVent @15:57:35.91 delay 10 seconds @15:57:45.94 AirS.reconfigure AirSconfig @15:57:46.03 AirS.seek :evac @15:57:47.51 Lysis Recovered in LyS1 @15:57:47.55 Lysis Complete @15:57:47.58 Handoff.select :waste @15:57:47.64 LyS2.seek :full @15:57:47.76 LyS1.reconfigure DAmixLyS1 @15:57:47.85 LyS2.reconfigure DAmixLyS2 @15:57:48.43 LyS2.seek 1480ul @15:57:48.48 LyS1.seek 650ul @15:58:35.85 delay 5 seconds @15:58:40.87 Handoff.select :SPR @15:58:41.05 SPR.command "start 1 14" @15:58:41.59 Running Experiment-Rep #1 @15:58:41.60 delay 10 seconds @15:58:51.63 SPR.command "start 1 0" @15:58:54.95 Waiting up to 4:00 for SPR to enter state 1 @16:00:59.25 SPR.command "hold_on" @16:01:01.08 LyS2.seek 1130ul @16:01:01.14 LyS1.seek 300ul @16:02:07.39 delay 15 seconds @16:02:22.43 SPR.command "hold_off" @16:02:25.88 Waiting up to 25:00 for SPR to enter state 7 @16:22:30.29 Handoff.select :waste @16:22:30.44 LyS1.reconfigure LyS1config @16:22:30.85 LyS2.reconfigure LyS2config @16:22:30.93 Rep #1 SPR Analysis has Completed @16:22:31.21 Ended log /var/log/esp/spr18jul2615h.spr @16:22:31.25 SPR.command "stats_off" @16:22:31.76 Handoff.select :close @16:22:32.00
PV.seek :locked @16:22:33.05 ArS.seek :clear @16:22:33.24 LyS1.seek :clear @16:22:33.42 LyS2.seek :clear @16:22:33.62 AirS.seek :clear @16:22:50.85
ArS.coast @16:22:50.94 LyS1.coast @16:22:51.01 LyS2.coast @16:22:51.08 AirS.coast @16:22:51.15 TV.coast @16:22:51.24 SP.coast @16:22:51.32 SC.coast @16:22:51.39 PV.coast @16:22:51.46 PinPort.coast @16:22:51.69 Gate.power :motor,:OFF @16:22:51.71 Gate.power :heat,:OFF @16:22:51.75 Gate.power :main,:OFF @16:22:51.80 Gate.power :cartridge,:OFF @16:22:51.83 Gate.power :core,:OFF @16:22:51.86 Gate.power :analytic1,:OFF @16:22:51.90 Gate.power :analytic2,:OFF @16:22:51.94 Gate.power :analytic3,:OFF @16:22:51.97 Safely stopped and ready to power off @16:22:52.18 delay 30 seconds @16:23:22.33 Gate.power :main,:ON,limit to 5000mA @16:23:22.35 Gate.power :analytic1,:ON,limit to 5000mA @16:23:22.39 Gate.power :analytic2,:ON,limit to 5000mA @16:23:22.42 Gate.power :analytic3,:ON,limit to 5000mA @16:23:22.54 Powering core bus @16:23:24.90 SPR Module 1, FW Rev. B, FPGA Rev. C @16:23:25.09
ArS.configure ArSconfig @16:23:25.24 LyS1.configure LyS1config @16:23:25.41 LyS2.configure LyS2config @16:23:25.55 AirS.configure AirSconfig @16:23:25.72 TV.configure TVconfig @16:23:25.90 SP.configure SPconfig @16:23:26.07 SC.configure SCconfig @16:23:26.25 PV.configure PVconfig @16:23:26.44 PinPort.configure PPconfig @16:23:26.60 Sea.configure SPconfig @16:23:26.69 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @16:23:26.72 Gate.power :motor,:ON,limit to 5000mA @16:23:27.43 PV.reconfigure PVconfig @16:23:27.47 TV.reconfigure TVconfig @16:23:27.51 LyS2.reconfigure LyS2config @16:23:27.57 PinPort.reconfigure PPconfig @16:23:27.63 AirS.reconfigure AirSconfig @16:23:27.68 LyS1.reconfigure LyS1config @16:23:27.72 ArS.reconfigure ArSconfig @16:23:28.03 PV.home.jog 0 @16:23:28.12 TV.home.jog 0 @16:23:28.15 LyS2.home.jog 0 @16:23:28.20 PinPort.home.jog 0 @16:23:28.26 AirS.home.jog 0 @16:23:28.30 LyS1.home.jog 0 @16:23:28.41 ArS.home.jog 0 @16:23:29.82 PV.reconfigure HomePV @16:23:29.91 TV.reconfigure HomeTV @16:23:29.95 LyS2.reconfigure HomeSyringe @16:23:30.01 LyS1.reconfigure HomeSyringe @16:23:30.07 PinPort.reconfigure HomePP @16:23:30.11 AirS.reconfigure HomeMagSyringe @16:23:30.20 ArS.reconfigure HomeMagSyringe @16:23:30.55 PV.jog -1498 @16:23:30.59 TV.jog -545 @16:23:30.64 LyS2.jog -262 @16:23:30.71 LyS1.jog -268 @16:23:30.76 PinPort.jog -2410 @16:23:30.83 ArS.jog -274 @16:23:30.88 AirS.jog -307 @16:23:31.55 LyS2.setRawPosition! -50 @16:23:31.65 LyS2.seek :home @16:23:31.81 AirS.setRawPosition! -50 @16:23:31.85 LyS1.setRawPosition! -50 @16:23:31.90 ArS.setRawPosition! -50 @16:23:32.07 AirS.seek :home @16:23:32.16 LyS1.seek :home @16:23:32.30 ArS.seek :home @16:23:32.50 PV.setRawPosition! -50 @16:23:32.53 LyS2.reconfigure LyS2config @16:23:32.73 PV.seek :home @16:23:32.89 AirS.reconfigure AirSconfig @16:23:32.94 LyS1.reconfigure LyS1config @16:23:33.01 ArS.reconfigure ArSconfig @16:23:33.28 PV.reconfigure PVconfig @16:23:33.31 TV.setRawPosition! 0 @16:23:33.47 TV.seek :home @16:23:33.61 TV.reconfigure TVconfig @16:23:36.83 PinPort.setRawPosition! 0 @16:23:36.90 PinPort.seek :home @16:23:37.01 PinPort.reconfigure PPconfig @16:23:37.60
TV.seek :clear @16:23:39.24 TV.coast @16:23:39.32 SC.reconfigure HomeSC @16:23:39.40 SC.home.jog 0 @16:23:40.18 SC.reconfigure SCconfig @16:23:40.28 SC.seek 20 @16:23:40.40 Selecting Cartridge 18 @16:23:40.82 SC.seek 18 @16:23:41.59 LyS2.seek :locked @16:23:41.79 PV.seek :locked @16:23:41.98 AirS.seek :locked @16:23:46.10
Powering cartridge bus @16:23:48.25 Gate.setIR 1750,5000,0,6 @16:23:48.37 Gate.setIR 0,5000,0,6 @16:23:48.45 Loaded Lyse-n-Go Cartridge @16:23:48.47 PuckHeater.configure HeatConfig @16:23:48.56 Gate.power :heat,:ON,limit to 5000mA @16:23:48.87 Duration of filtering limited to 1:05:00 @16:23:49.21 Priming sample loop w/100ml, bypass w/10ml @16:23:49.66 SP.setPosition! 0ml @16:23:49.72 Intake.open @16:23:49.76 Exhaust.open @16:23:50.66 Sea @-42.9psia @16:23:50.68 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @16:23:56.46 Vacuum tare is 20% of full scale @16:23:56.48 PB.open @16:23:56.51 Exhaust.close @16:23:57.40 Sea @-42.9psia @16:23:57.53 SP.reconfigure SPprime @16:23:57.63 SP.seek 10ml,1:21 @16:24:08.53 SP.setPosition! 0ml @16:24:08.61 PB.close @16:24:08.64 Exhaust.open @16:24:09.50 Sea @-42.9psia @16:24:09.63 SP.reconfigure SPprime @16:24:09.72 SP.seek 100ml,8:51 @16:25:56.25 Pressurizing cartridge to 26psi @16:25:56.27 Intake.close @16:25:57.07 SP.setPosition! 0ml @16:25:57.15 PB.open @16:25:57.96 TV.seek :crack @16:25:58.79 TV.seek :clear @16:25:59.61 Intake.open @16:26:01.04 PB.close @16:26:01.76 Exhaust.close @16:26:02.55 Sea @-42.9psia @16:26:02.68 SP.reconfigure SPcharge @16:26:02.77 SP.seek 7.5ml,1:08 @16:26:16.12 Cartridge pressurized with 0.830ml to 26.1psi @16:26:16.43 delay 3 seconds @16:26:19.52 Cartridge at 23.3psi 3 seconds later @16:26:19.65 TV.seek :cartridge @16:26:21.49 TV.seek :clear @16:26:23.33 Sampling 500.0ml @16:26:23.35 SP.setPosition! 0ml @16:26:23.43 Exhaust.open @16:26:24.25 Sea @-42.9psia @16:26:24.39 SP.reconfigure SPsample @16:26:24.48 SP.seek 500ml,42:12 @16:26:25.42 Cartridge 18 sampling 0/500ml at 0.15ml/s with 3.0psi vacuum @16:27:25.42 Cartridge 18 sampling 36/500ml at 0.60ml/s with 24.2psi vacuum @16:28:25.42 Cartridge 18 sampling 71/500ml at 0.57ml/s with 23.7psi vacuum @16:29:25.43 Cartridge 18 sampling 106/500ml at 0.58ml/s with 21.6psi vacuum @16:30:25.42 Cartridge 18 sampling 141/500ml at 0.58ml/s with 23.2psi vacuum @16:31:25.42 Cartridge 18 sampling 176/500ml at 0.57ml/s with 25.8psi vacuum @16:32:25.43 Cartridge 18 sampling 210/500ml at 0.56ml/s with 23.4psi vacuum @16:33:25.41 Cartridge 18 sampling 245/500ml at 0.58ml/s with 25.1psi vacuum @16:34:25.42 Cartridge 18 sampling 279/500ml at 0.56ml/s with 21.7psi vacuum @16:35:25.77 Cartridge 18 sampling 313/500ml at 0.55ml/s with 21.6psi vacuum @16:36:25.42 Cartridge 18 sampling 347/500ml at 0.55ml/s with 24.7psi vacuum @16:37:25.42 Cartridge 18 sampling 380/500ml at 0.57ml/s with 18.5psi vacuum @16:38:25.42 Cartridge 18 sampling 413/500ml at 0.53ml/s with 25.7psi vacuum @16:39:25.42 Cartridge 18 sampling 446/500ml at 0.52ml/s with 20.5psi vacuum @16:40:25.42 Cartridge 18 sampling 478/500ml at 0.56ml/s with 24.3psi vacuum @16:41:07.52 Intake.close @16:41:07.58 Exhaust.close @16:41:08.41 Sampled 500.0ml @16:41:08.53 TV.seek :bypass @16:41:10.19 TV.seek :clear @16:41:13.67 SPR.command "read_sequences" @16:41:13.73 SPR.command "stats_on" @16:41:14.54 SPR.command "start 2 0" @16:41:16.54 Begun log /var/log/esp/spr18jul2616h.spr @16:41:17.38 Handoff.select :waste @16:41:17.54 ArS.seek empty @16:41:35.29 PinPort.seek :cartridge @16:41:41.04 LyS1.seek 1350ul @16:41:47.00 PV.seek :reagent @16:41:47.88 AirS.seek :tVent @16:41:49.97 delay 2 seconds @16:41:52.00 AirS.reconfigure EvacAirS @16:41:52.09 AirS.seek :evac @16:42:14.42 delay 15 seconds @16:42:29.45 AirS.reconfigure AirSconfig @16:42:29.54 AirS.seek :tVent @16:42:43.78 delay 2 seconds @16:42:45.82 AirS.reconfigure EvacAirS @16:42:45.92 AirS.seek :evac @16:43:08.25 delay 15 seconds @16:43:23.29 AirS.reconfigure AirSconfig @16:43:23.37 AirS.seek :tVent @16:43:37.60 delay 2 seconds @16:43:39.63 AirS.reconfigure EvacAirS @16:43:39.72 AirS.seek :evac @16:44:02.08 delay 15 seconds @16:44:17.11 AirS.reconfigure AirSconfig @16:44:17.20 AirS.seek :tVent @16:44:31.43 delay 2 seconds @16:44:33.82 AirS.reconfigure EvacAirS @16:44:33.91 AirS.seek :evac @16:44:56.21 delay 15 seconds @16:45:11.25 AirS.reconfigure AirSconfig @16:45:11.34 AirS.seek :tVent @16:45:25.56 delay 2 seconds @16:45:27.60 AirS.reconfigure EvacAirS @16:45:27.69 AirS.seek :evac @16:45:50.02 delay 15 seconds @16:46:05.05 delay 1:30 @16:47:35.07 AirS.reconfigure AirSconfig @16:47:35.16 AirS.seek :fVent @16:47:36.62 delay 15 seconds @16:47:51.66 LyS1.reconfigure SlowLyS1 @16:47:51.80 LyS1.seek 1050ul @16:48:21.86 delay 5 seconds @16:48:26.88 Handoff.select :blocked @16:48:27.11 LyS1.seek 450ul @16:49:27.08 delay 10 seconds @16:49:37.11 AirS.reconfigure SlowAirS @16:49:37.29 AirS.reconfigure to mix into 1.37806 parts LyS1 @16:49:37.86 AirS.seek evac @16:49:37.91 LyS1.seek 300ul @16:49:53.08 AirS.reconfigure AirSconfig @16:49:53.18 AirS.seek :full @16:50:07.41 LyS1.seek :empty @16:50:37.52 PuckHeater.seek 84.3C @16:51:37.50 PuckHeater.hold 15:00,84.3C @17:06:34.34 LyS1.reconfigure LyS1config @17:06:34.44 LyS1.seek 900ul @17:06:42.80 AirS.seek :tVent @17:06:42.92 delay 2 seconds @17:06:44.95 AirS.reconfigure HalfSpeedAirS @17:06:45.05 AirS.seek :evac @17:07:12.83 delay 15 seconds @17:07:27.88 AirS.reconfigure AirSconfig @17:07:28.07 AirS.seek :tVent @17:07:42.31 delay 2 seconds @17:07:44.34 AirS.reconfigure HalfSpeedAirS @17:07:44.43 AirS.seek :evac @17:08:12.21 delay 15 seconds @17:08:27.25 AirS.reconfigure AirSconfig @17:08:27.33 AirS.seek :tVent @17:08:41.58 delay 2 seconds @17:08:43.62 AirS.reconfigure HalfSpeedAirS @17:08:43.70 AirS.seek :evac @17:09:11.48 delay 15 seconds @17:09:26.51 delay 1:00 @17:10:26.53 Handoff.select :waste @17:10:26.68 delay 15 seconds @17:10:41.72 AirS.seek :fVent @17:10:43.95 delay 10 seconds @17:10:53.98 AirS.reconfigure AirSconfig @17:10:54.06 AirS.seek :evac @17:10:55.56 Lysis Recovered in LyS1 @17:10:55.57 Lysis Complete @17:10:55.60 Handoff.select :waste @17:10:55.66 LyS2.seek :full @17:10:55.78 LyS1.reconfigure DAmixLyS1 @17:10:55.87 LyS2.reconfigure DAmixLyS2 @17:10:56.44 LyS2.seek 1480ul @17:10:56.54 LyS1.seek 650ul @17:11:43.87 delay 5 seconds @17:11:48.89 Handoff.select :SPR @17:11:49.03 SPR.command "start 1 14" @17:11:50.90 Running Experiment-Rep #1 @17:11:50.92 delay 10 seconds @17:12:00.97 SPR.command "start 1 0" @17:12:03.77 Waiting up to 4:00 for SPR to enter state 1 @17:14:08.04 SPR.command "hold_on" @17:14:09.85 LyS2.seek 1130ul @17:14:09.90 LyS1.seek 300ul @17:15:16.15 delay 15 seconds @17:15:31.17 SPR.command "hold_off" @17:15:35.18 Waiting up to 25:00 for SPR to enter state 7 @17:35:40.10 Handoff.select :waste @17:35:40.25 LyS1.reconfigure LyS1config @17:35:40.33 LyS2.reconfigure LyS2config @17:35:40.41 Rep #1 SPR Analysis has Completed @17:35:40.96 Ended log /var/log/esp/spr18jul2616h.spr @17:35:40.98 SPR.command "stats_off" @17:35:41.49 Handoff.select :close @17:35:41.73
PV.seek :locked @17:35:42.75 ArS.seek :clear @17:35:42.95 LyS1.seek :clear @17:35:43.13 LyS2.seek :clear @17:35:43.32 AirS.seek :clear @17:36:00.54
ArS.coast @17:36:00.62 LyS1.coast @17:36:00.69 LyS2.coast @17:36:00.76 AirS.coast @17:36:00.84 TV.coast @17:36:00.92 SP.coast @17:36:00.99 SC.coast @17:36:01.07 PV.coast @17:36:01.14 PinPort.coast @17:36:01.36 Gate.power :motor,:OFF @17:36:01.38 Gate.power :heat,:OFF @17:36:01.40 Gate.power :main,:OFF @17:36:01.45 Gate.power :cartridge,:OFF @17:36:01.49 Gate.power :core,:OFF @17:36:01.52 Gate.power :analytic1,:OFF @17:36:01.56 Gate.power :analytic2,:OFF @17:36:01.61 Gate.power :analytic3,:OFF @17:36:01.63 Safely stopped and ready to power off @17:36:01.81 delay 30 seconds @17:36:31.94 Gate.power :main,:ON,limit to 5000mA @17:36:31.96 Gate.power :analytic1,:ON,limit to 5000mA @17:36:32.01 Gate.power :analytic2,:ON,limit to 5000mA @17:36:32.05 Gate.power :analytic3,:ON,limit to 5000mA @17:36:32.16 Powering core bus @17:36:34.52 SPR Module 1, FW Rev. B, FPGA Rev. C @17:36:34.70
ArS.configure ArSconfig @17:36:34.84 LyS1.configure LyS1config @17:36:35.01 LyS2.configure LyS2config @17:36:35.16 AirS.configure AirSconfig @17:36:35.33 TV.configure TVconfig @17:36:35.50 SP.configure SPconfig @17:36:35.66 SC.configure SCconfig @17:36:35.82 PV.configure PVconfig @17:36:35.99 PinPort.configure PPconfig @17:36:36.16 Sea.configure SPconfig @17:36:36.25 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @17:36:36.28 Gate.power :motor,:ON,limit to 5000mA @17:36:36.50 ArS.reconfigure ArSconfig @17:36:36.92 ArS.home.jog 0 @17:36:37.17 PinPort.reconfigure PPconfig @17:36:37.22 PV.reconfigure PVconfig @17:36:37.26 TV.reconfigure TVconfig @17:36:37.32 LyS2.reconfigure LyS2config @17:36:37.39 LyS1.reconfigure LyS1config @17:36:37.51 AirS.reconfigure AirSconfig @17:36:37.75 PV.home.jog 0 @17:36:37.80 TV.home.jog 0 @17:36:37.88 PinPort.home.jog 0 @17:36:37.93 LyS2.home.jog 0 @17:36:38.03 AirS.home.jog 0 @17:36:38.09 LyS1.home.jog 0 @17:36:38.62 ArS.reconfigure HomeMagSyringe @17:36:39.49 LyS1.reconfigure HomeSyringe @17:36:39.56 ArS.jog -274 @17:36:39.63 PV.reconfigure HomePV @17:36:39.68 TV.reconfigure HomeTV @17:36:39.74 AirS.reconfigure HomeMagSyringe @17:36:39.78 PinPort.reconfigure HomePP @17:36:39.86 LyS2.reconfigure HomeSyringe @17:36:40.14 LyS1.jog -274 @17:36:40.24 PV.jog -1497 @17:36:40.27 TV.jog -548 @17:36:40.33 PinPort.jog -2410 @17:36:40.39 AirS.jog -311 @17:36:40.52 LyS2.jog -262 @17:36:40.80 ArS.setRawPosition! -50 @17:36:40.88 ArS.seek :home @17:36:41.05 LyS1.setRawPosition! -50 @17:36:41.13 LyS1.seek :home @17:36:41.32 AirS.setRawPosition! -50 @17:36:41.36 LyS2.setRawPosition! -50 @17:36:41.40 ArS.reconfigure ArSconfig @17:36:41.58 AirS.seek :home @17:36:41.62 LyS2.seek :home @17:36:41.83 PV.setRawPosition! -50 @17:36:41.86 LyS1.reconfigure LyS1config @17:36:42.05 PV.seek :home @17:36:42.21 LyS2.reconfigure LyS2config @17:36:42.23 AirS.reconfigure AirSconfig @17:36:42.45 PV.reconfigure PVconfig @17:36:42.58 TV.setRawPosition! 0 @17:36:42.65 TV.seek :home @17:36:42.78 TV.reconfigure TVconfig @17:36:46.37 PinPort.setRawPosition! 0 @17:36:46.45 PinPort.seek :home @17:36:46.56 PinPort.reconfigure PPconfig @17:36:47.14
TV.seek :clear @17:36:48.76 TV.coast @17:36:48.85 SC.reconfigure HomeSC @17:36:48.93 SC.home.jog 0 @17:36:49.72 SC.reconfigure SCconfig @17:36:49.80 SC.seek 18 @17:36:49.93 Selecting Cartridge 16 @17:36:50.34 SC.seek 16 @17:36:51.11 LyS2.seek :locked @17:36:51.31 PV.seek :locked @17:36:51.49 AirS.seek :locked @17:36:55.61
Powering cartridge bus @17:36:57.75 Gate.setIR 1750,5000,0,6 @17:36:57.88 Gate.setIR 0,5000,0,6 @17:36:57.97 Loaded Lyse-n-Go Cartridge @17:36:57.98 PuckHeater.configure HeatConfig @17:36:58.07 Gate.power :heat,:ON,limit to 5000mA @17:36:58.72 Duration of filtering limited to 1:05:00 @17:36:59.05 Priming sample loop w/100ml, bypass w/10ml @17:36:59.17 SP.setPosition! 0ml @17:36:59.22 Intake.open @17:36:59.28 Exhaust.open @17:37:00.20 Sea @-42.9psia @17:37:00.22 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @17:37:05.97 Vacuum tare is 20% of full scale @17:37:05.99 PB.open @17:37:06.04 Exhaust.close @17:37:06.94 Sea @-42.9psia @17:37:07.06 SP.reconfigure SPprime @17:37:07.15 SP.seek 10ml,1:21 @17:37:18.04 SP.setPosition! 0ml @17:37:18.12 PB.close @17:37:18.15 Exhaust.open @17:37:19.08 Sea @-42.9psia @17:37:19.20 SP.reconfigure SPprime @17:37:19.30 SP.seek 100ml,8:51 @17:39:05.66 Pressurizing cartridge to 26psi @17:39:05.67 Intake.close @17:39:06.48 SP.setPosition! 0ml @17:39:06.55 PB.open @17:39:07.39 TV.seek :crack @17:39:08.21 TV.seek :clear @17:39:09.05 Intake.open @17:39:10.50 PB.close @17:39:11.26 Exhaust.close @17:39:12.09 Sea @-42.9psia @17:39:12.21 SP.reconfigure SPcharge @17:39:12.31 SP.seek 7.5ml,1:08 @17:39:20.46 Cartridge pressurized with 0.307ml to 26.1psi @17:39:20.75 Pressure peaked at 26.4psi @17:39:20.78 delay 3 seconds @17:39:23.88 Cartridge at 19.6psi 3 seconds later @17:39:24.00 TV.seek :cartridge @17:39:25.85 TV.seek :clear @17:39:27.69 Sampling 500.0ml @17:39:27.71 SP.setPosition! 0ml @17:39:27.80 Exhaust.open @17:39:28.64 Sea @-42.9psia @17:39:28.77 SP.reconfigure SPsample @17:39:28.86 SP.seek 500ml,42:12 @17:39:29.80 Cartridge 16 sampling 0/500ml at 0.15ml/s with 2.5psi vacuum @17:40:29.81 Cartridge 16 sampling 36/500ml at 0.63ml/s with 22.6psi vacuum @17:41:29.80 Cartridge 16 sampling 72/500ml at 0.62ml/s with 22.8psi vacuum @17:42:29.80 Cartridge 16 sampling 109/500ml at 0.61ml/s with 20.5psi vacuum @17:43:29.81 Cartridge 16 sampling 144/500ml at 0.62ml/s with 22.1psi vacuum @17:44:29.81 Cartridge 16 sampling 180/500ml at 0.58ml/s with 25.8psi vacuum @17:45:30.16 Cartridge 16 sampling 215/500ml at 0.56ml/s with 26.3psi vacuum @17:46:29.81 Cartridge 16 sampling 250/500ml at 0.60ml/s with 26.8psi vacuum @17:47:29.81 Cartridge 16 sampling 285/500ml at 0.58ml/s with 21.4psi vacuum @17:48:29.82 Cartridge 16 sampling 319/500ml at 0.55ml/s with 25.9psi vacuum @17:49:29.81 Cartridge 16 sampling 353/500ml at 0.59ml/s with 24.5psi vacuum @17:50:29.81 Cartridge 16 sampling 387/500ml at 0.58ml/s with 21.8psi vacuum @17:51:29.81 Cartridge 16 sampling 420/500ml at 0.55ml/s with 20.9psi vacuum @17:52:29.81 Cartridge 16 sampling 453/500ml at 0.53ml/s with 26.0psi vacuum @17:53:29.81 Cartridge 16 sampling 486/500ml at 0.54ml/s with 20.8psi vacuum @17:54:00.58 Intake.close @17:54:00.62 Exhaust.close @17:54:01.47 Sampled 500.0ml @17:54:01.60 TV.seek :bypass @17:54:03.24 TV.seek :clear @17:54:06.72 SPR.command "read_sequences" @17:54:07.12 SPR.command "stats_on" @17:54:07.18 SPR.command "start 2 0" @17:54:09.19 Begun log /var/log/esp/spr18jul2617h.spr @17:54:09.97 Handoff.select :waste @17:54:10.13 ArS.seek empty @17:54:27.87 PinPort.seek :cartridge @17:54:33.63 LyS1.seek 1350ul @17:54:39.60 PV.seek :reagent @17:54:40.47 AirS.seek :tVent @17:54:42.57 delay 2 seconds @17:54:44.60 AirS.reconfigure EvacAirS @17:54:44.68 AirS.seek :evac @17:55:07.00 delay 15 seconds @17:55:22.03 AirS.reconfigure AirSconfig @17:55:22.14 AirS.seek :tVent @17:55:36.38 delay 2 seconds @17:55:38.42 AirS.reconfigure EvacAirS @17:55:38.52 AirS.seek :evac @17:56:00.84 delay 15 seconds @17:56:15.87 AirS.reconfigure AirSconfig @17:56:15.96 AirS.seek :tVent @17:56:30.20 delay 2 seconds @17:56:32.23 AirS.reconfigure EvacAirS @17:56:32.33 AirS.seek :evac @17:56:54.68 delay 15 seconds @17:57:09.71 AirS.reconfigure AirSconfig @17:57:09.80 AirS.seek :tVent @17:57:24.04 delay 2 seconds @17:57:26.07 AirS.reconfigure EvacAirS @17:57:26.15 AirS.seek :evac @17:57:48.51 delay 15 seconds @17:58:03.54 AirS.reconfigure AirSconfig @17:58:03.63 AirS.seek :tVent @17:58:17.86 delay 2 seconds @17:58:19.89 AirS.reconfigure EvacAirS @17:58:19.97 AirS.seek :evac @17:58:42.29 delay 15 seconds @17:58:57.32 delay 1:30 @18:00:27.35 AirS.reconfigure AirSconfig @18:00:27.44 AirS.seek :fVent @18:00:28.90 delay 15 seconds @18:00:43.94 LyS1.reconfigure SlowLyS1 @18:00:44.09 LyS1.seek 1050ul @18:01:14.14 delay 5 seconds @18:01:19.16 Handoff.select :blocked @18:01:19.40 LyS1.seek 450ul @18:02:19.39 delay 10 seconds @18:02:29.42 AirS.reconfigure SlowAirS @18:02:29.58 AirS.reconfigure to mix into 1.37806 parts LyS1 @18:02:30.13 AirS.seek evac @18:02:30.19 LyS1.seek 300ul @18:02:45.37 AirS.reconfigure AirSconfig @18:02:45.46 AirS.seek :full @18:02:59.71 LyS1.seek :empty @18:03:29.84 PuckHeater.seek 84.2C @18:04:27.68 PuckHeater.hold 15:00,84.2C @18:19:28.82 LyS1.reconfigure LyS1config @18:19:28.92 LyS1.seek 900ul @18:19:37.27 AirS.seek :tVent @18:19:37.39 delay 2 seconds @18:19:39.42 AirS.reconfigure HalfSpeedAirS @18:19:39.50 AirS.seek :evac @18:20:07.27 delay 15 seconds @18:20:22.32 AirS.reconfigure AirSconfig @18:20:22.51 AirS.seek :tVent @18:20:36.75 delay 2 seconds @18:20:38.78 AirS.reconfigure HalfSpeedAirS @18:20:38.87 AirS.seek :evac @18:21:06.62 delay 15 seconds @18:21:21.65 AirS.reconfigure AirSconfig @18:21:21.74 AirS.seek :tVent @18:21:35.99 delay 2 seconds @18:21:38.02 AirS.reconfigure HalfSpeedAirS @18:21:38.12 AirS.seek :evac @18:22:05.89 delay 15 seconds @18:22:20.92 delay 1:00 @18:23:20.93 Handoff.select :waste @18:23:21.09 delay 15 seconds @18:23:36.12 AirS.seek :fVent @18:23:38.35 delay 10 seconds @18:23:48.38 AirS.reconfigure AirSconfig @18:23:48.46 AirS.seek :evac @18:23:49.95 Lysis Recovered in LyS1 @18:23:49.96 Lysis Complete @18:23:49.98 Handoff.select :waste @18:23:50.05 LyS2.seek :full @18:23:50.16 LyS1.reconfigure DAmixLyS1 @18:23:50.25 LyS2.reconfigure DAmixLyS2 @18:23:50.80 LyS2.seek 1480ul @18:23:50.83 LyS1.seek 650ul @18:24:38.20 delay 5 seconds @18:24:43.25 Handoff.select :SPR @18:24:43.37 SPR.command "start 1 14" @18:24:44.45 Running Experiment-Rep #1 @18:24:44.46 delay 10 seconds @18:24:54.49 SPR.command "start 1 0" @18:24:57.29 Waiting up to 4:00 for SPR to enter state 1 @18:27:01.58 SPR.command "hold_on" @18:27:03.90 LyS2.seek 1130ul @18:27:03.95 LyS1.seek 300ul @18:28:10.17 delay 15 seconds @18:28:25.19 SPR.command "hold_off" @18:28:28.21 Waiting up to 25:00 for SPR to enter state 7 @18:48:31.74 Handoff.select :waste @18:48:31.90 LyS1.reconfigure LyS1config @18:48:31.98 LyS2.reconfigure LyS2config @18:48:32.07 Rep #1 SPR Analysis has Completed @18:48:32.61 Ended log /var/log/esp/spr18jul2617h.spr @18:48:32.63 SPR.command "stats_off" @18:48:33.65 Handoff.select :close @18:48:33.89
PV.seek :locked @18:48:34.90 ArS.seek :clear @18:48:35.11 LyS1.seek :clear @18:48:35.32 LyS2.seek :clear @18:48:35.49 AirS.seek :clear @18:48:52.71
ArS.coast @18:48:52.78 LyS1.coast @18:48:52.86 LyS2.coast @18:48:52.92 AirS.coast @18:48:53.00 TV.coast @18:48:53.07 SP.coast @18:48:53.15 SC.coast @18:48:53.24 PV.coast @18:48:53.31 PinPort.coast @18:48:53.53 Gate.power :motor,:OFF @18:48:53.55 Gate.power :heat,:OFF @18:48:53.59 Gate.power :main,:OFF @18:48:53.64 Gate.power :cartridge,:OFF @18:48:53.66 Gate.power :core,:OFF @18:48:53.68 Gate.power :analytic1,:OFF @18:48:53.73 Gate.power :analytic2,:OFF @18:48:53.77 Gate.power :analytic3,:OFF @18:48:53.80 Safely stopped and ready to power off @18:48:54.02 delay 30 seconds @18:49:24.05 No dry cartridges remain @18:49:24.14 Session Ended @19:15:45.52PDT26-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @19:15:45.54 >BREAK< ESP3 Gateway v0.99 for cartridge bus @19:15:47.08 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @19:15:48.51 Polling Can every 5:00 @19:15:48.56 Loading /home/esp/esp2/mission/processAll.rb mission @19:15:48.59 Processing all remaining dry cartridges in: {dry: [12, 14]} @19:15:48.60 Sampling maximum of 500.0ml per cartridge @19:15:48.62 Fixing samples for 10:00, evacuating once @19:15:48.63 delay 30 seconds @19:16:18.76 Gate.power :main,:ON,limit to 5000mA @19:16:18.78 Gate.power :analytic1,:ON,limit to 5000mA @19:16:18.79 Gate.power :analytic2,:ON,limit to 5000mA @19:16:18.80 Gate.power :analytic3,:ON,limit to 5000mA @19:16:18.96 Powering core bus @19:16:21.28 SPR Module 1, FW Rev. B, FPGA Rev. C @19:16:21.39
ArS.configure ArSconfig @19:16:21.51 LyS1.configure LyS1config @19:16:21.65 LyS2.configure LyS2config @19:16:21.76 AirS.configure AirSconfig @19:16:21.91 TV.configure TVconfig @19:16:22.04 SP.configure SPconfig @19:16:22.18 SC.configure SCconfig @19:16:22.33 PV.configure PVconfig @19:16:22.47 PinPort.configure PPconfig @19:16:22.62 Sea.configure SPconfig @19:16:22.68 All elf controllers running firmware version 4.46 @19:16:22.70 Gate.power :motor,:ON,limit to 5000mA @19:16:22.86 ArS.reconfigure ArSconfig @19:16:23.23 ArS.home.jog 0 @19:16:23.51 LyS2.reconfigure LyS2config @19:16:23.57 PV.reconfigure PVconfig @19:16:23.63 TV.reconfigure TVconfig @19:16:23.67 LyS1.reconfigure LyS1config @19:16:23.70 PinPort.reconfigure PPconfig @19:16:23.75 AirS.reconfigure AirSconfig @19:16:23.98 LyS2.home.jog 0 @19:16:24.09 PV.home.jog 0 @19:16:24.12 LyS1.home.jog 0 @19:16:24.15 PinPort.home.jog 0 @19:16:24.18 TV.home.jog 0 @19:16:24.23 AirS.home.jog 0 @19:16:24.65 ArS.reconfigure HomeMagSyringe @19:16:24.97 ArS.jog -273 @19:16:25.17 PinPort.reconfigure HomePP @19:16:25.23 PV.reconfigure HomePV @19:16:25.30 LyS2.reconfigure HomeSyringe @19:16:25.37 LyS1.reconfigure HomeSyringe @19:16:25.40 TV.reconfigure HomeTV @19:16:25.57 PinPort.jog -109 @19:16:25.67 LyS2.jog -269 @19:16:25.71 PV.jog -248 @19:16:25.75 LyS1.jog -275 @19:16:25.81 TV.jog -549 @19:16:26.07 ArS.setRawPosition! -50 @19:16:26.12 AirS.reconfigure HomeMagSyringe @19:16:26.17 ArS.seek :home @19:16:26.29 AirS.jog -301 @19:16:26.41 PinPort.setRawPosition! 0 @19:16:26.48 PinPort.seek :home @19:16:26.62 PV.setRawPosition! -50 @19:16:26.66 LyS2.setRawPosition! -50 @19:16:26.68 LyS1.setRawPosition! -50 @19:16:26.70 ArS.reconfigure ArSconfig @19:16:26.72 PinPort.reconfigure PPconfig @19:16:27.00 PV.seek :home @19:16:27.05 LyS2.seek :home @19:16:27.09 LyS1.seek :home @19:16:27.67 AirS.setRawPosition! -50 @19:16:27.72 AirS.seek :home @19:16:27.90 TV.setRawPosition! 0 @19:16:27.96 LyS2.reconfigure LyS2config @19:16:27.98 PV.reconfigure PVconfig @19:16:28.03 LyS1.reconfigure LyS1config @19:16:28.21 TV.seek :home @19:16:28.38 AirS.reconfigure AirSconfig @19:16:28.41 TV.reconfigure TVconfig @19:16:29.05
TV.seek :clear @19:16:30.66 TV.coast @19:16:30.71 SC.reconfigure HomeSC @19:16:30.77 SC.home.jog 0 @19:16:31.55 SC.reconfigure SCconfig @19:16:31.61 SC.seek 16 @19:16:31.71 Selecting Cartridge 14 @19:16:32.08 SC.seek 14 @19:16:32.84 LyS2.seek :locked @19:16:32.99 PV.seek :locked @19:16:33.16 AirS.seek :locked @19:16:37.25
Powering cartridge bus @19:16:39.38 Gate.setIR 1750,5000,0,6 @19:16:39.50 Gate.setIR 0,5000,0,6 @19:16:39.56 Loaded Lyse-n-Go Cartridge @19:16:39.57 PuckHeater.configure HeatConfig @19:16:39.61 Gate.power :heat,:ON,limit to 5000mA @19:16:39.91 Duration of filtering limited to 1:05:00 @19:16:40.21 Priming sample loop w/100ml, bypass w/10ml @19:16:40.30 SP.setPosition! 0ml @19:16:40.35 Intake.open @19:16:40.38 Exhaust.open @19:16:41.22 Sea @-42.9psia @19:16:41.24 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @19:16:47.01 Vacuum tare is 20% of full scale @19:16:47.04 PB.open @19:16:47.08 Exhaust.close @19:16:47.88 Sea @-42.9psia @19:16:47.97 SP.reconfigure SPprime @19:16:48.04 SP.seek 10ml,1:21 @19:16:58.91 SP.setPosition! 0ml @19:16:58.95 PB.close @19:16:59.00 Exhaust.open @19:16:59.83 Sea @-42.9psia @19:16:59.93 SP.reconfigure SPprime @19:16:59.99 SP.seek 100ml,8:51 @19:18:45.62 Pressurizing cartridge to 26psi @19:18:45.64 Intake.close @19:18:46.35 SP.setPosition! 0ml @19:18:46.39 PB.open @19:18:47.20 TV.seek :crack @19:18:48.01 TV.seek :clear @19:18:48.82 Intake.open @19:18:50.24 PB.close @19:18:51.03 Exhaust.close @19:18:51.81 Sea @-42.9psia @19:18:51.90 SP.reconfigure SPcharge @19:18:51.97 SP.seek 7.5ml,1:08 @19:19:02.17 Cartridge pressurized with 0.487ml to 26.4psi @19:19:02.44 delay 3 seconds @19:19:05.53 Cartridge at 22.8psi 3 seconds later @19:19:05.62 TV.seek :cartridge @19:19:07.43 TV.seek :clear @19:19:09.24 Sampling 500.0ml @19:19:09.26 SP.setPosition! 0ml @19:19:09.30 Exhaust.open @19:19:10.07 Sea @-42.9psia @19:19:10.17 SP.reconfigure SPsample @19:19:10.25 SP.seek 500ml,42:12 @19:19:11.24 Cartridge 14 sampling 0/500ml at 0.17ml/s with 2.1psi vacuum @19:20:11.22 Cartridge 14 sampling 37/500ml at 0.61ml/s with 24.1psi vacuum @19:21:11.23 Cartridge 14 sampling 75/500ml at 0.64ml/s with 23.7psi vacuum @19:22:11.23 Cartridge 14 sampling 112/500ml at 0.61ml/s with 20.9psi vacuum @19:23:11.22 Cartridge 14 sampling 150/500ml at 0.62ml/s with 21.5psi vacuum @19:24:11.22 Cartridge 14 sampling 187/500ml at 0.64ml/s with 23.7psi vacuum @19:25:11.24 Cartridge 14 sampling 225/500ml at 0.65ml/s with 22.8psi vacuum @19:26:11.24 Cartridge 14 sampling 263/500ml at 0.63ml/s with 21.5psi vacuum @19:27:11.24 Cartridge 14 sampling 302/500ml at 0.66ml/s with 19.9psi vacuum @19:28:11.24 Cartridge 14 sampling 341/500ml at 0.65ml/s with 24.7psi vacuum @19:29:11.24 Cartridge 14 sampling 380/500ml at 0.65ml/s with 25.2psi vacuum @19:30:11.23 Cartridge 14 sampling 419/500ml at 0.65ml/s with 22.2psi vacuum @19:31:11.22 Cartridge 14 sampling 459/500ml at 0.66ml/s with 22.7psi vacuum @19:32:11.24 Cartridge 14 sampling 498/500ml at 0.66ml/s with 24.1psi vacuum @19:32:13.84 Intake.close @19:32:13.88 Exhaust.close @19:32:14.67 Sampled 500.0ml @19:32:14.76 TV.seek :bypass @19:32:16.38 TV.seek :clear @19:32:19.83 SPR.command "read_sequences" @19:32:20.22 SPR.command "stats_on" @19:32:20.26 SPR.command "start 2 0" @19:32:22.26 Begun log /var/log/esp/spr18jul2619h.spr AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @19:32:25.48 Handoff.select :waste @19:32:25.60 ArS.seek empty @19:32:43.33 PinPort.seek :cartridge @19:32:49.05 LyS1.seek 1350ul @19:32:55.05 PV.seek :reagent @19:32:55.89 AirS.seek :tVent @19:32:57.96 delay 2 seconds @19:32:59.99 AirS.reconfigure EvacAirS @19:33:00.04 AirS.seek :evac @19:33:22.35 delay 15 seconds @19:33:37.38 AirS.reconfigure AirSconfig @19:33:37.44 AirS.seek :tVent @19:33:51.65 delay 2 seconds @19:33:53.68 AirS.reconfigure EvacAirS @19:33:53.73 AirS.seek :evac @19:34:16.03 delay 15 seconds @19:34:31.06 AirS.reconfigure AirSconfig @19:34:31.11 AirS.seek :tVent @19:34:45.33 delay 2 seconds @19:34:47.36 AirS.reconfigure EvacAirS @19:34:47.41 AirS.seek :evac @19:35:09.72 delay 15 seconds @19:35:24.75 AirS.reconfigure AirSconfig @19:35:24.80 AirS.seek :tVent @19:35:39.02 delay 2 seconds @19:35:41.05 AirS.reconfigure EvacAirS @19:35:41.12 AirS.seek :evac @19:36:03.42 delay 15 seconds @19:36:18.46 AirS.reconfigure AirSconfig @19:36:18.51 AirS.seek :tVent @19:36:32.74 delay 2 seconds @19:36:34.77 AirS.reconfigure EvacAirS @19:36:34.82 AirS.seek :evac @19:36:57.12 delay 15 seconds @19:37:12.15 delay 1:30 @19:38:42.17 AirS.reconfigure AirSconfig @19:38:42.30 AirS.seek :fVent @19:38:43.74 delay 15 seconds @19:38:58.78 LyS1.reconfigure SlowLyS1 @19:38:58.91 LyS1.seek 1050ul @19:39:28.95 delay 5 seconds @19:39:33.97 Handoff.select :blocked @19:39:34.15 LyS1.seek 450ul @19:40:34.12 delay 10 seconds @19:40:44.15 AirS.reconfigure SlowAirS @19:40:44.29 AirS.reconfigure to mix into 1.37806 parts LyS1 @19:40:44.74 AirS.seek evac @19:40:44.80 LyS1.seek 300ul @19:40:59.91 AirS.reconfigure AirSconfig @19:41:00.00 AirS.seek :full @19:41:14.22 LyS1.seek :empty @19:41:44.31 PuckHeater.seek 84.3C @19:42:42.09 PuckHeater.hold 15:00,84.3C @19:57:44.30 LyS1.reconfigure LyS1config @19:57:44.37 LyS1.seek 900ul @19:57:52.71 AirS.seek :tVent @19:57:52.80 delay 2 seconds @19:57:54.85 AirS.reconfigure HalfSpeedAirS @19:57:54.91 AirS.seek :evac @19:58:22.64 delay 15 seconds @19:58:37.67 AirS.reconfigure AirSconfig @19:58:37.75 AirS.seek :tVent @19:58:52.23 delay 2 seconds @19:58:54.34 AirS.reconfigure HalfSpeedAirS @19:58:54.39 AirS.seek :evac @19:59:22.16 delay 15 seconds @19:59:37.19 AirS.reconfigure AirSconfig @19:59:37.25 AirS.seek :tVent @19:59:51.46 delay 2 seconds @19:59:53.50 AirS.reconfigure HalfSpeedAirS @19:59:53.56 AirS.seek :evac @20:00:21.31 delay 15 seconds @20:00:36.34 delay 1:00 @20:01:36.35 Handoff.select :waste @20:01:36.47 delay 15 seconds @20:01:51.50 AirS.seek :fVent @20:01:53.70 delay 10 seconds @20:02:03.73 AirS.reconfigure AirSconfig @20:02:03.80 AirS.seek :evac @20:02:05.24 Lysis Recovered in LyS1 @20:02:05.25 Lysis Complete @20:02:05.26 Handoff.select :waste @20:02:05.28 LyS2.seek :full @20:02:05.36 LyS1.reconfigure DAmixLyS1 @20:02:05.43 LyS2.reconfigure DAmixLyS2 @20:02:05.95 LyS2.seek 1480ul @20:02:05.99 LyS1.seek 650ul @20:02:53.34 delay 5 seconds @20:02:58.36 Handoff.select :SPR @20:02:58.47 SPR.command "start 1 14" @20:03:00.38 Running Experiment-Rep #1 @20:03:00.39 delay 10 seconds @20:03:10.41 SPR.command "start 1 0" @20:03:13.22 Waiting up to 4:00 for SPR to enter state 1 @20:05:17.51 SPR.command "hold_on" @20:05:19.34 LyS2.seek 1130ul @20:05:19.40 LyS1.seek 300ul @20:06:25.57 delay 15 seconds @20:06:40.60 SPR.command "hold_off" @20:06:43.71 Waiting up to 25:00 for SPR to enter state 7 @20:26:48.56 Handoff.select :waste @20:26:48.68 LyS1.reconfigure LyS1config @20:26:48.74 LyS2.reconfigure LyS2config @20:26:49.11 Rep #1 SPR Analysis has Completed @20:26:49.48 Ended log /var/log/esp/spr18jul2619h.spr @20:26:49.51 SPR.command "stats_off" @20:26:50.04 Handoff.select :close @20:26:50.25
PV.seek :locked @20:26:51.30 ArS.seek :clear @20:26:51.41 LyS1.seek :clear @20:26:51.59 LyS2.seek :clear @20:26:51.78 AirS.seek :clear @20:27:09.01
ArS.coast @20:27:09.05 LyS1.coast @20:27:09.10 LyS2.coast @20:27:09.14 AirS.coast @20:27:09.19 TV.coast @20:27:09.23 SP.coast @20:27:09.27 SC.coast @20:27:09.32 PV.coast @20:27:09.36 PinPort.coast @20:27:09.56 Gate.power :motor,:OFF @20:27:09.58 Gate.power :heat,:OFF @20:27:09.59 Gate.power :main,:OFF @20:27:09.60 Gate.power :cartridge,:OFF @20:27:09.62 Gate.power :core,:OFF @20:27:09.63 Gate.power :analytic1,:OFF @20:27:09.64 Gate.power :analytic2,:OFF @20:27:09.65 Gate.power :analytic3,:OFF @20:27:09.66 Safely stopped and ready to power off @20:27:09.80 delay 30 seconds @20:27:39.91 Gate.power :main,:ON,limit to 5000mA @20:27:39.93 Gate.power :analytic1,:ON,limit to 5000mA @20:27:39.94 Gate.power :analytic2,:ON,limit to 5000mA @20:27:39.95 Gate.power :analytic3,:ON,limit to 5000mA @20:27:40.02 Powering core bus @20:27:42.40 SPR Module 1, FW Rev. B, FPGA Rev. C @20:27:42.55
ArS.configure ArSconfig @20:27:42.67 LyS1.configure LyS1config @20:27:42.81 LyS2.configure LyS2config @20:27:42.93 AirS.configure AirSconfig @20:27:43.08 TV.configure TVconfig @20:27:43.21 SP.configure SPconfig @20:27:43.35 SC.configure SCconfig @20:27:43.50 PV.configure PVconfig @20:27:43.64 PinPort.configure PPconfig @20:27:43.77 Sea.configure SPconfig @20:27:43.83 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @20:27:43.85 Gate.power :motor,:ON,limit to 5000mA @20:27:44.00 ArS.reconfigure ArSconfig @20:27:44.36 ArS.home.jog 0 @20:27:44.60 PinPort.reconfigure PPconfig @20:27:44.70 TV.reconfigure TVconfig @20:27:44.76 PV.reconfigure PVconfig @20:27:44.83 LyS2.reconfigure LyS2config @20:27:44.87 LyS1.reconfigure LyS1config @20:27:44.91 AirS.reconfigure AirSconfig @20:27:45.18 TV.home.jog 0 @20:27:45.22 PinPort.home.jog 0 @20:27:45.25 PV.home.jog 0 @20:27:45.28 LyS2.home.jog 0 @20:27:45.31 LyS1.home.jog 0 @20:27:45.34 AirS.home.jog 0 @20:27:46.08 ArS.reconfigure HomeMagSyringe @20:27:46.33 ArS.jog -275 @20:27:46.41 PV.reconfigure HomePV @20:27:46.44 AirS.reconfigure HomeMagSyringe @20:27:46.49 LyS1.reconfigure HomeSyringe @20:27:46.53 TV.reconfigure HomeTV @20:27:46.58 LyS2.reconfigure HomeSyringe @20:27:46.97 PinPort.reconfigure HomePP @20:27:47.24 PV.jog -1501 @20:27:47.32 LyS1.jog -274 @20:27:47.41 PinPort.jog -2410 @20:27:47.46 TV.jog -549 @20:27:47.49 AirS.jog -308 @20:27:47.53 LyS2.jog -262 @20:27:47.83 ArS.setRawPosition! -50 @20:27:47.88 ArS.seek :home @20:27:48.18 ArS.reconfigure ArSconfig @20:27:48.29 LyS1.setRawPosition! -50 @20:27:48.34 LyS1.seek :home @20:27:48.43 LyS2.setRawPosition! -50 @20:27:48.46 AirS.setRawPosition! -50 @20:27:48.57 LyS2.seek :home @20:27:48.61 AirS.seek :home @20:27:48.77 LyS1.reconfigure LyS1config @20:27:48.80 PV.setRawPosition! -50 @20:27:48.92 PV.seek :home @20:27:49.06 AirS.reconfigure AirSconfig @20:27:49.10 LyS2.reconfigure LyS2config @20:27:49.25 PV.reconfigure PVconfig @20:27:49.60 TV.setRawPosition! 0 @20:27:49.65 TV.seek :home @20:27:49.76 TV.reconfigure TVconfig @20:27:53.34 PinPort.setRawPosition! 0 @20:27:53.39 PinPort.seek :home @20:27:53.47 PinPort.reconfigure PPconfig @20:27:54.02
TV.seek :clear @20:27:55.63 TV.coast @20:27:55.68 SC.reconfigure HomeSC @20:27:55.74 SC.home.jog 0 @20:27:56.51 SC.reconfigure SCconfig @20:27:56.56 SC.seek 14 @20:27:56.66 Selecting Cartridge 12 @20:27:57.02 SC.seek 12 @20:27:57.78 LyS2.seek :locked @20:27:57.93 PV.seek :locked @20:27:58.08 AirS.seek :locked @20:28:02.18
Powering cartridge bus @20:28:04.31 Gate.setIR 1750,5000,0,6 @20:28:04.43 Gate.setIR 0,5000,0,6 @20:28:04.49 Loaded Lyse-n-Go Cartridge @20:28:04.50 PuckHeater.configure HeatConfig @20:28:04.54 Gate.power :heat,:ON,limit to 5000mA @20:28:04.83 Duration of filtering limited to 1:05:00 @20:28:05.13 Priming sample loop w/100ml, bypass w/10ml @20:28:05.22 SP.setPosition! 0ml @20:28:05.27 Intake.open @20:28:05.32 Exhaust.open @20:28:06.12 Sea @-42.9psia @20:28:06.14 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @20:28:12.21 Vacuum tare is 21% of full scale @20:28:12.22 PB.open @20:28:12.26 Exhaust.close @20:28:13.14 Sea @-42.9psia @20:28:13.25 SP.reconfigure SPprime @20:28:13.32 SP.seek 10ml,1:21 @20:28:24.20 SP.setPosition! 0ml @20:28:24.25 PB.close @20:28:24.28 Exhaust.open @20:28:25.13 Sea @-42.9psia @20:28:25.22 SP.reconfigure SPprime @20:28:25.29 SP.seek 100ml,8:51 @20:30:11.03 Pressurizing cartridge to 26psi @20:30:11.04 Intake.close @20:30:11.77 SP.setPosition! 0ml @20:30:11.81 PB.open @20:30:12.64 TV.seek :crack @20:30:13.44 TV.seek :clear @20:30:14.24 Intake.open @20:30:15.70 PB.close @20:30:16.42 Exhaust.close @20:30:17.21 Sea @-42.9psia @20:30:17.30 SP.reconfigure SPcharge @20:30:17.36 SP.seek 7.5ml,1:08 @20:30:29.32 Cartridge pressurized with 0.668ml to 26.4psi @20:30:29.58 delay 3 seconds @20:30:32.68 Cartridge at 22.9psi 3 seconds later @20:30:32.77 TV.seek :cartridge @20:30:34.59 TV.seek :clear @20:30:36.41 Sampling 500.0ml @20:30:36.42 SP.setPosition! 0ml @20:30:36.46 Exhaust.open @20:30:37.25 Sea @-42.9psia @20:30:37.35 SP.reconfigure SPsample @20:30:37.41 SP.seek 500ml,42:12 @20:30:38.41 Cartridge 12 sampling 0/500ml at 0.17ml/s with 3.3psi vacuum @20:31:38.41 Cartridge 12 sampling 37/500ml at 0.63ml/s with 23.8psi vacuum @20:32:38.42 Cartridge 12 sampling 75/500ml at 0.64ml/s with 23.3psi vacuum @20:33:38.42 Cartridge 12 sampling 113/500ml at 0.64ml/s with 20.9psi vacuum @20:34:38.42 Cartridge 12 sampling 150/500ml at 0.64ml/s with 23.4psi vacuum @20:35:38.41 Cartridge 12 sampling 188/500ml at 0.65ml/s with 22.5psi vacuum @20:36:38.42 Cartridge 12 sampling 227/500ml at 0.65ml/s with 23.1psi vacuum @20:37:38.42 Cartridge 12 sampling 265/500ml at 0.65ml/s with 23.3psi vacuum @20:38:38.42 Cartridge 12 sampling 304/500ml at 0.66ml/s with 23.6psi vacuum @20:39:38.42 Cartridge 12 sampling 343/500ml at 0.66ml/s with 23.9psi vacuum @20:40:38.42 Cartridge 12 sampling 383/500ml at 0.66ml/s with 23.5psi vacuum @20:41:38.42 Cartridge 12 sampling 422/500ml at 0.66ml/s with 24.6psi vacuum @20:42:38.43 Cartridge 12 sampling 462/500ml at 0.66ml/s with 23.9psi vacuum @20:43:36.73 Intake.close @20:43:36.79 Exhaust.close @20:43:37.56 Sampled 500.0ml @20:43:37.65 TV.seek :bypass @20:43:39.27 TV.seek :clear @20:43:42.73 SPR.command "read_sequences" @20:43:43.26 SPR.command "stats_on" @20:43:43.30 SPR.command "start 2 0" @20:43:45.30 Begun log /var/log/esp/spr18jul2620h.spr @20:43:46.11 Handoff.select :waste @20:43:46.25 ArS.seek empty @20:44:03.98 PinPort.seek :cartridge @20:44:09.70 LyS1.seek 1350ul @20:44:15.64 PV.seek :reagent @20:44:16.49 AirS.seek :tVent @20:44:18.55 delay 2 seconds @20:44:20.58 AirS.reconfigure EvacAirS @20:44:20.64 AirS.seek :evac @20:44:42.96 delay 15 seconds @20:44:57.99 AirS.reconfigure AirSconfig @20:44:58.04 AirS.seek :tVent @20:45:12.26 delay 2 seconds @20:45:14.29 AirS.reconfigure EvacAirS @20:45:14.34 AirS.seek :evac @20:45:36.65 delay 15 seconds @20:45:51.68 AirS.reconfigure AirSconfig @20:45:51.74 AirS.seek :tVent @20:46:05.96 delay 2 seconds @20:46:07.99 AirS.reconfigure EvacAirS @20:46:08.05 AirS.seek :evac @20:46:30.35 delay 15 seconds @20:46:45.38 AirS.reconfigure AirSconfig @20:46:45.44 AirS.seek :tVent @20:46:59.65 delay 2 seconds @20:47:01.68 AirS.reconfigure EvacAirS @20:47:01.73 AirS.seek :evac @20:47:24.04 delay 15 seconds @20:47:39.07 AirS.reconfigure AirSconfig @20:47:39.12 AirS.seek :tVent @20:47:53.34 delay 2 seconds @20:47:55.37 AirS.reconfigure EvacAirS @20:47:55.42 AirS.seek :evac @20:48:17.73 delay 15 seconds @20:48:32.76 delay 1:30 @20:50:02.78 AirS.reconfigure AirSconfig @20:50:02.83 AirS.seek :fVent @20:50:04.27 delay 15 seconds @20:50:19.30 LyS1.reconfigure SlowLyS1 @20:50:19.43 LyS1.seek 1050ul @20:50:49.47 delay 5 seconds @20:50:54.49 Handoff.select :blocked @20:50:54.67 LyS1.seek 450ul @20:51:54.64 delay 10 seconds @20:52:04.69 AirS.reconfigure SlowAirS @20:52:04.82 AirS.reconfigure to mix into 1.37806 parts LyS1 @20:52:05.32 AirS.seek evac @20:52:05.37 LyS1.seek 300ul @20:52:20.49 AirS.reconfigure AirSconfig @20:52:20.55 AirS.seek :full @20:52:34.76 LyS1.seek :empty @20:53:04.85 PuckHeater.seek 84.3C @20:54:03.92 PuckHeater.hold 15:00,84.3C @21:09:03.93 LyS1.reconfigure LyS1config @21:09:04.00 LyS1.seek 900ul @21:09:12.33 AirS.seek :tVent @21:09:12.42 delay 2 seconds @21:09:14.45 AirS.reconfigure HalfSpeedAirS @21:09:14.50 AirS.seek :evac @21:09:42.25 delay 15 seconds @21:09:57.28 AirS.reconfigure AirSconfig @21:09:57.60 AirS.seek :tVent @21:10:11.91 delay 2 seconds @21:10:13.94 AirS.reconfigure HalfSpeedAirS @21:10:13.99 AirS.seek :evac @21:10:41.74 delay 15 seconds @21:10:56.77 AirS.reconfigure AirSconfig @21:10:56.83 AirS.seek :tVent @21:11:11.05 delay 2 seconds @21:11:13.08 AirS.reconfigure HalfSpeedAirS @21:11:13.13 AirS.seek :evac @21:11:40.88 delay 15 seconds @21:11:55.91 delay 1:00 @21:12:55.93 Handoff.select :waste @21:12:56.05 delay 15 seconds @21:13:11.08 AirS.seek :fVent @21:13:13.28 delay 10 seconds @21:13:23.31 AirS.reconfigure AirSconfig @21:13:23.36 AirS.seek :evac @21:13:24.80 Lysis Recovered in LyS1 @21:13:24.81 Lysis Complete @21:13:24.82 Handoff.select :waste @21:13:24.84 LyS2.seek :full @21:13:24.92 LyS1.reconfigure DAmixLyS1 @21:13:24.98 LyS2.reconfigure DAmixLyS2 @21:13:25.49 LyS2.seek 1480ul @21:13:25.55 LyS1.seek 650ul @21:14:12.86 delay 5 seconds @21:14:17.88 Handoff.select :SPR @21:14:17.99 SPR.command "start 1 14" @21:14:19.63 Running Experiment-Rep #1 @21:14:19.65 delay 10 seconds @21:14:29.67 SPR.command "start 1 0" @21:14:33.00 Waiting up to 4:00 for SPR to enter state 1 @21:16:37.71 SPR.command "hold_on" @21:16:39.54 LyS2.seek 1130ul @21:16:39.59 LyS1.seek 300ul @21:17:45.79 delay 15 seconds @21:18:00.82 SPR.command "hold_off" @21:18:03.91 Waiting up to 25:00 for SPR to enter state 7 @21:38:08.83 Handoff.select :waste @21:38:08.95 LyS1.reconfigure LyS1config @21:38:09.01 LyS2.reconfigure LyS2config @21:38:09.08 Rep #1 SPR Analysis has Completed @21:38:09.69 Ended log /var/log/esp/spr18jul2620h.spr @21:38:09.71 SPR.command "stats_off" @21:38:10.23 Handoff.select :close @21:38:10.43
PV.seek :locked @21:38:11.43 ArS.seek :clear @21:38:11.59 LyS1.seek :clear @21:38:11.76 LyS2.seek :clear @21:38:11.96 AirS.seek :clear @21:38:29.18
ArS.coast @21:38:29.23 LyS1.coast @21:38:29.27 LyS2.coast @21:38:29.32 AirS.coast @21:38:29.36 TV.coast @21:38:29.41 SP.coast @21:38:29.45 SC.coast @21:38:29.50 PV.coast @21:38:29.55 PinPort.coast @21:38:29.75 Gate.power :motor,:OFF @21:38:29.77 Gate.power :heat,:OFF @21:38:29.78 Gate.power :main,:OFF @21:38:29.79 Gate.power :cartridge,:OFF @21:38:29.80 Gate.power :core,:OFF @21:38:29.82 Gate.power :analytic1,:OFF @21:38:29.83 Gate.power :analytic2,:OFF @21:38:29.84 Gate.power :analytic3,:OFF @21:38:29.85 Safely stopped and ready to power off @21:38:30.09 delay 30 seconds @21:39:00.13 No dry cartridges remain @09:39:13.76PDT27-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @09:39:13.79 >BREAK< ESP3 Gateway v0.99 for cartridge bus @09:39:15.39 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @09:39:16.93 Polling Can every 5:00 @09:39:17.05 Gate.power :analytic1,:ON,limit to 5000mA @09:39:17.06 Gate.power :analytic2,:ON,limit to 5000mA @09:39:17.08 Gate.power :analytic3,:ON,limit to 5000mA @09:39:17.78 ArS.configure ArSconfig @09:39:17.90 SPR Module 1, FW Rev. B, FPGA Rev. C @09:39:17.97
LyS1.configure LyS1config @09:39:18.11 LyS2.configure LyS2config @09:39:18.21 AirS.configure AirSconfig @09:39:18.35 TV.configure TVconfig @09:39:18.49 SP.configure SPconfig @09:39:18.67 SC.reconfigure SCconfig @09:39:18.79 SC.hold @09:39:18.92 SC.configure SCconfig @09:39:19.19 PV.configure PVconfig @09:39:19.47 PinPort.configure PPconfig @09:39:19.74 Sea.configure SPconfig @09:39:19.87 All elf controllers running firmware version 4.46 @09:39:20.03 Intake.close @09:39:20.17 Exhaust.close @09:39:20.26 PB.close @09:39:20.69
Loading /home/esp/esp2/mission/processAll.rb mission @09:39:20.77 Processing all remaining dry cartridges in: {dry: 11} @09:39:20.79 NoMethodError in MAIN -- undefined method `goalVolume' for Cmd:Module @09:51:26.11PDT27-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @09:51:26.14 >BREAK< ESP3 Gateway v0.99 for cartridge bus @09:51:27.72 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @09:51:29.60 Polling Can every 5:00 @09:51:29.81 Gate.power :analytic1,:ON,limit to 5000mA @09:51:29.84 Gate.power :analytic2,:ON,limit to 5000mA @09:51:29.87 Gate.power :analytic3,:ON,limit to 5000mA @09:51:30.56 ArS.configure ArSconfig @09:51:30.68 LyS1.configure LyS1config @09:51:30.81 LyS2.configure LyS2config @09:51:30.91 SPR Module 1, FW Rev. B, FPGA Rev. C @09:51:31.01
AirS.configure AirSconfig @09:51:31.14 TV.configure TVconfig @09:51:31.28 SP.configure SPconfig @09:51:31.42 SC.reconfigure SCconfig @09:51:31.48 SC.hold @09:51:31.53 SC.configure SCconfig @09:51:31.67 PV.configure PVconfig @09:51:31.80 PinPort.configure PPconfig @09:51:31.93 Sea.configure SPconfig @09:51:31.99 All elf controllers running firmware version 4.46 @09:51:32.06 Intake.close @09:51:32.13 Exhaust.close @09:51:32.19 PB.close @09:51:32.38
Loading /home/esp/esp2/mission/processAll.rb mission @09:51:32.40 Processing all remaining dry cartridges in: {dry: 11} @09:51:32.41 Sampling default goal volume per cartridge type loaded @09:51:32.43 Fixing samples for 10:00, evacuating once @09:51:32.44 delay 30 seconds @09:51:32.99 Session Begun @09:51:52.98 -> showlog 40 @09:52:03.26
SC.seek 14 @09:52:03.38 Selecting Cartridge 11 @09:52:03.76 SC.seek 11 @09:52:04.73 LyS2.seek :locked @09:52:04.91 PV.seek :locked @09:52:05.09 AirS.seek :locked @09:52:09.18
Powering cartridge bus @09:52:10.49 -> Cmd.slots @09:52:11.32
Gate.setIR 1750,5000,0,6 @09:52:11.45 Gate.setIR 0,5000,0,6 @09:52:11.51 Loaded Archive Cartridge @09:52:11.52 Gate.power :cartridge,:OFF @09:52:11.87 Duration of filtering limited to 1:05:00 @09:52:12.20 Priming sample loop w/100ml, bypass w/10ml @09:52:12.33 SP.setPosition! 0ml @09:52:12.38 Intake.open @09:52:12.43 Exhaust.open @09:52:13.34 Sea @-42.9psia @09:52:13.36 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @09:52:19.44 Vacuum tare is 21% of full scale @09:52:19.46 PB.open @09:52:19.49 Exhaust.close @09:52:20.38 Sea @-42.9psia @09:52:20.50 SP.reconfigure SPprime @09:52:20.60 SP.seek 10ml,1:21 @09:52:31.50 SP.setPosition! 0ml @09:52:31.58 PB.close @09:52:31.61 Exhaust.open @09:52:32.51 Sea @-42.9psia @09:52:32.64 SP.reconfigure SPprime @09:52:32.73 SP.seek 100ml,8:51 @09:54:19.17 Pressurizing cartridge to 26psi @09:54:19.19 Intake.close @09:54:19.99 SP.setPosition! 0ml @09:54:20.06 PB.open @09:54:20.90 TV.seek :crack @09:54:21.73 TV.seek :clear @09:54:22.56 Intake.open @09:54:24.02 PB.close @09:54:24.76 Exhaust.close @09:54:25.59 Sea @-42.9psia @09:54:25.71 SP.reconfigure SPcharge @09:54:25.80 SP.seek 7.5ml,1:08 @09:54:39.25 Cartridge pressurized with 0.843ml to 26.1psi @09:54:39.55 delay 3 seconds @09:54:42.64 Cartridge at 22.6psi 3 seconds later @09:54:42.77 TV.seek :cartridge @09:54:44.61 TV.seek :clear @09:54:46.46 Sampling 1000.0ml @09:54:46.48 SP.setPosition! 0ml @09:54:46.57 Exhaust.open @09:54:47.37 Sea @-42.9psia @09:54:47.50 SP.reconfigure SPsample @09:54:47.60 SP.seek 1000ml,1:23:53 @09:54:48.54 Cartridge 11 sampling 0/1000ml at 0.15ml/s with 7.3psi vacuum @09:55:48.53 Cartridge 11 sampling 25/1000ml at 0.41ml/s with 25.0psi vacuum @09:56:48.53 Cartridge 11 sampling 50/1000ml at 0.41ml/s with 21.9psi vacuum @09:57:48.53 Cartridge 11 sampling 74/1000ml at 0.40ml/s with 25.9psi vacuum @09:58:48.53 Cartridge 11 sampling 97/1000ml at 0.41ml/s with 23.9psi vacuum @09:59:48.53 Cartridge 11 sampling 119/1000ml at 0.39ml/s with 22.6psi vacuum @10:00:48.53 Cartridge 11 sampling 141/1000ml at 0.38ml/s with 24.6psi vacuum @10:01:48.54 Cartridge 11 sampling 161/1000ml at 0.35ml/s with 21.7psi vacuum @10:02:48.52 Cartridge 11 sampling 181/1000ml at 0.29ml/s with 20.7psi vacuum @10:03:48.53 Cartridge 11 sampling 200/1000ml at 0.28ml/s with 20.1psi vacuum @10:04:48.53 Cartridge 11 sampling 218/1000ml at 0.29ml/s with 26.8psi vacuum @10:05:48.52 Cartridge 11 sampling 235/1000ml at 0.24ml/s with 24.6psi vacuum @10:06:48.53 Cartridge 11 sampling 252/1000ml at 0.32ml/s with 21.6psi vacuum @10:07:39.89 Session Begun @10:07:45.84 -> showlog 10 @10:07:48.53 Cartridge 11 sampling 268/1000ml at 0.21ml/s with 23.0psi vacuum @10:08:48.53 Cartridge 11 sampling 283/1000ml at 0.22ml/s with 26.5psi vacuum @10:09:48.54 Cartridge 11 sampling 298/1000ml at 0.27ml/s with 20.6psi vacuum @10:10:48.88 Cartridge 11 sampling 312/1000ml at 0.28ml/s with 23.9psi vacuum @10:11:48.53 Cartridge 11 sampling 326/1000ml at 0.20ml/s with 24.5psi vacuum @10:12:23.86 -> Cmd.slots @10:12:48.54 Cartridge 11 sampling 339/1000ml at 0.20ml/s with 23.2psi vacuum @10:13:48.53 Cartridge 11 sampling 353/1000ml at 0.24ml/s with 23.3psi vacuum @10:14:48.53 Cartridge 11 sampling 365/1000ml at 0.20ml/s with 24.0psi vacuum @10:15:47.81 -> Cartridge.sampler.vacuum @10:15:48.53 Cartridge 11 sampling 378/1000ml at 0.20ml/s with 25.3psi vacuum @10:16:48.53 Cartridge 11 sampling 390/1000ml at 0.20ml/s with 24.5psi vacuum @10:17:48.53 Cartridge 11 sampling 402/1000ml at 0.20ml/s with 26.7psi vacuum @10:18:48.53 Cartridge 11 sampling 415/1000ml at 0.20ml/s with 28.1psi vacuum @10:19:48.53 Cartridge 11 sampling 427/1000ml at 0.20ml/s with 23.9psi vacuum @10:20:48.53 Cartridge 11 sampling 439/1000ml at 0.20ml/s with 29.1psi vacuum @10:21:48.53 Cartridge 11 sampling 451/1000ml at 0.20ml/s with 26.0psi vacuum @10:22:21.03 Flow averaged 0.28ml/s over 27:32 @10:22:21.08 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @10:22:21.76 Relieved excess vacuum in 3 seconds @10:22:21.82 SP.seek 1000ml,45:41 @10:22:48.52 Cartridge 11 sampling 463/1000ml at 0.20ml/s with 27.1psi vacuum @10:22:58.98 Flow averaged 0.20ml/s over 37 seconds @10:22:59.01 Intake.close @10:22:59.07 Exhaust.close @10:22:59.92 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:23:00.07 Sampled 465.4ml @10:23:00.21 TV.seek :bypass @10:23:01.88 TV.seek :clear @10:23:03.84 PV.seek :reagent @10:23:04.74 Sample loop closed. Ready to Deliver Reagents @10:23:04.76 ArS.seek :sealed @10:23:09.10 ArS.seek :evac @10:23:23.09 delay 20 seconds @10:23:43.13 ArS.seek :clear @10:24:00.90 RNAlater soak for 10:00 @10:34:00.94 ArS.seek :tVent @10:34:03.57 delay 1 second @10:34:04.60 ArS.seek :evac @10:34:20.27 delay 10 seconds @10:34:30.31 Sample preserved and evacuated @10:34:30.51
PV.seek :locked @10:34:31.54 ArS.seek :clear @10:34:31.80 LyS2.seek :clear @10:34:32.03 AirS.seek :clear @10:34:49.35
ArS.coast @10:34:49.46 LyS2.coast @10:34:49.54 AirS.coast @10:34:49.63 TV.coast @10:34:49.71 SP.coast @10:34:49.81 SC.coast @10:34:49.90 PV.coast @10:34:50.14 Gate.power :motor,:OFF @10:34:50.16 Gate.power :main,:OFF @10:34:50.21 Gate.power :core,:OFF @10:34:50.26 Gate.power :analytic1,:OFF @10:34:50.29 Gate.power :analytic2,:OFF @10:34:50.35 Gate.power :analytic3,:OFF @10:34:50.39 Safely stopped and ready to power off @10:34:50.62 delay 30 seconds @10:35:20.65 No dry cartridges remain @10:35:20.76 Session Ended @10:35:20.81 Session Ended @10:40:02.72PDT27-Jul-18
>BREAK< ESP3 Gateway v0.99 for core bus @10:40:02.75 >BREAK< ESP3 Gateway v0.99 for cartridge bus @10:40:04.28 Configuration for honu by brent@mbari.org /home/esp/esp2/type/auv/honu/configure.rb @10:40:05.72 Polling Can every 5:00 @10:40:05.78 Loading /home/esp/esp2/mission/processAll.rb mission @10:40:05.82 Processing all remaining dry cartridges in: {1 => :spare, 465.426 => 11, dry: [8..10]} @10:40:05.84 Sampling default goal volume per cartridge type loaded @10:40:05.89 Fixing samples for 10:00, evacuating once @10:40:05.96 delay 30 seconds @10:40:06.00 Session Begun @10:40:36.10
Gate.power :main,:ON,limit to 5000mA @10:40:36.12 Gate.power :analytic1,:ON,limit to 5000mA @10:40:36.18 Gate.power :analytic2,:ON,limit to 5000mA @10:40:36.20 Gate.power :analytic3,:ON,limit to 5000mA @10:40:36.38 Powering core bus @10:40:38.66 SPR Module 1, FW Rev. B, FPGA Rev. C @10:40:38.85
ArS.configure ArSconfig @10:40:38.98 LyS1.configure LyS1config @10:40:39.14 LyS2.configure LyS2config @10:40:39.29 AirS.configure AirSconfig @10:40:39.45 TV.configure TVconfig @10:40:39.61 SP.configure SPconfig @10:40:39.77 SC.configure SCconfig @10:40:39.93 PV.configure PVconfig @10:40:40.09 PinPort.configure PPconfig @10:40:40.26 Sea.configure SPconfig @10:40:40.33 All elf controllers running firmware version 4.46 @10:40:40.36 Gate.power :motor,:ON,limit to 5000mA @10:40:40.77 ArS.reconfigure ArSconfig @10:40:41.06 PV.reconfigure PVconfig @10:40:41.15 LyS2.reconfigure LyS2config @10:40:41.20 ArS.home.jog 0 @10:40:41.28 PinPort.reconfigure PPconfig @10:40:41.31 AirS.reconfigure AirSconfig @10:40:41.37 TV.reconfigure TVconfig @10:40:41.48 LyS1.reconfigure LyS1config @10:40:41.73 PV.home.jog 0 @10:40:41.81 LyS2.home.jog 0 @10:40:41.86 PinPort.home.jog 0 @10:40:41.91 AirS.home.jog 0 @10:40:41.94 TV.home.jog 0 @10:40:42.04 LyS1.home.jog 0 @10:40:43.04 PinPort.reconfigure HomePP @10:40:43.07 PV.reconfigure HomePV @10:40:43.12 TV.reconfigure HomeTV @10:40:43.21 LyS2.reconfigure HomeSyringe @10:40:43.29 LyS1.reconfigure HomeSyringe @10:40:43.36 ArS.reconfigure HomeMagSyringe @10:40:43.40 AirS.reconfigure HomeMagSyringe @10:40:43.69 PV.jog -1493 @10:40:43.81 PinPort.jog -109 @10:40:43.83 TV.jog -549 @10:40:43.89 LyS2.jog -264 @10:40:43.94 LyS1.jog -274 @10:40:43.98 ArS.jog -275 @10:40:44.07 AirS.jog -297 @10:40:44.49 PinPort.setRawPosition! 0 @10:40:44.57 PinPort.seek :home @10:40:44.69 PinPort.reconfigure PPconfig @10:40:44.81 LyS2.setRawPosition! -50 @10:40:44.92 LyS2.seek :home @10:40:45.04 AirS.setRawPosition! -50 @10:40:45.08 LyS1.setRawPosition! -50 @10:40:45.45 ArS.setRawPosition! -50 @10:40:45.62 AirS.seek :home @10:40:45.67 LyS1.seek :home @10:40:45.73 ArS.seek :home @10:40:46.02 PV.setRawPosition! -50 @10:40:46.06 LyS2.reconfigure LyS2config @10:40:46.22 PV.seek :home @10:40:46.41 TV.setRawPosition! 0 @10:40:46.45 LyS1.reconfigure LyS1config @10:40:46.50 ArS.reconfigure ArSconfig @10:40:46.55 AirS.reconfigure AirSconfig @10:40:46.78 TV.seek :home @10:40:46.96 PV.reconfigure PVconfig @10:40:46.99 TV.reconfigure TVconfig @10:40:47.34
TV.seek :clear @10:40:48.98 TV.coast @10:40:49.05 SC.reconfigure HomeSC @10:40:49.14 SC.home.jog 0 @10:40:49.93 SC.reconfigure SCconfig @10:40:50.02 SC.seek 11 @10:40:50.14 Selecting Cartridge 10 @10:40:50.54 SC.seek 10 @10:40:51.10 LyS2.seek :locked @10:40:51.30 PV.seek :locked @10:40:51.48 AirS.seek :locked @10:40:55.60
Powering cartridge bus @10:40:57.73 Gate.setIR 1750,5000,0,6 @10:40:57.85 Gate.setIR 0,5000,0,6 @10:40:57.94 Loaded Lyse-n-Go Cartridge @10:40:57.95 PuckHeater.configure HeatConfig @10:40:58.04 Gate.power :heat,:ON,limit to 5000mA @10:40:58.35 Duration of filtering limited to 1:05:00 @10:40:58.68 Priming sample loop w/100ml, bypass w/10ml @10:40:58.80 SP.setPosition! 0ml @10:40:58.86 Intake.open @10:40:58.91 Exhaust.open @10:40:59.76 Sea @-42.9psia @10:40:59.78 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @10:41:05.56 Vacuum tare is 21% of full scale @10:41:05.58 PB.open @10:41:05.67 Exhaust.close @10:41:06.54 Sea @-42.9psia @10:41:06.67 SP.reconfigure SPprime @10:41:06.76 SP.seek 10ml,1:21 @10:41:18.01 SP.setPosition! 0ml @10:41:18.09 PB.close @10:41:18.12 Exhaust.open @10:41:19.05 Sea @-42.9psia @10:41:19.18 SP.reconfigure SPprime @10:41:19.28 SP.seek 100ml,8:51 @10:43:06.08 Pressurizing cartridge to 26psi @10:43:06.09 Intake.close @10:43:06.88 SP.setPosition! 0ml @10:43:06.95 PB.open @10:43:07.82 TV.seek :crack @10:43:08.65 TV.seek :clear @10:43:09.48 Intake.open @10:43:10.89 PB.close @10:43:11.65 Exhaust.close @10:43:12.49 Sea @-42.9psia @10:43:12.62 SP.reconfigure SPcharge @10:43:12.71 SP.seek 7.5ml,1:08 @10:43:25.91 Cartridge pressurized with 0.812ml to 27.2psi @10:43:26.20 delay 3 seconds @10:43:29.30 Cartridge at 23.0psi 3 seconds later @10:43:29.41 TV.seek :cartridge @10:43:31.27 TV.seek :clear @10:43:33.12 Sampling 800.0ml @10:43:33.13 SP.setPosition! 0ml @10:43:33.22 Exhaust.open @10:43:34.05 Sea @-42.9psia @10:43:34.18 SP.reconfigure SPsample @10:43:34.27 SP.seek 800ml,1:07:12 @10:43:35.21 Cartridge 10 sampling 0/800ml at 0.16ml/s with 2.9psi vacuum @10:44:35.21 Cartridge 10 sampling 35/800ml at 0.58ml/s with 24.7psi vacuum @10:45:35.21 Cartridge 10 sampling 71/800ml at 0.58ml/s with 20.8psi vacuum @10:46:35.22 Cartridge 10 sampling 107/800ml at 0.61ml/s with 22.8psi vacuum @10:47:35.21 Cartridge 10 sampling 142/800ml at 0.59ml/s with 19.2psi vacuum @10:48:35.21 Cartridge 10 sampling 177/800ml at 0.60ml/s with 22.4psi vacuum @10:49:35.21 Cartridge 10 sampling 212/800ml at 0.56ml/s with 25.8psi vacuum @10:50:35.20 Cartridge 10 sampling 247/800ml at 0.60ml/s with 24.1psi vacuum @10:51:35.22 Cartridge 10 sampling 281/800ml at 0.57ml/s with 26.4psi vacuum @10:52:35.23 Cartridge 10 sampling 316/800ml at 0.59ml/s with 26.5psi vacuum @10:53:35.22 Cartridge 10 sampling 350/800ml at 0.59ml/s with 21.9psi vacuum @10:54:35.22 Cartridge 10 sampling 384/800ml at 0.58ml/s with 21.0psi vacuum @10:55:35.20 Cartridge 10 sampling 417/800ml at 0.55ml/s with 21.6psi vacuum @10:56:35.22 Cartridge 10 sampling 450/800ml at 0.57ml/s with 26.4psi vacuum @10:57:35.22 Cartridge 10 sampling 483/800ml at 0.54ml/s with 27.3psi vacuum @10:58:35.22 Cartridge 10 sampling 515/800ml at 0.51ml/s with 20.4psi vacuum @10:59:35.21 Cartridge 10 sampling 547/800ml at 0.57ml/s with 21.6psi vacuum @11:00:35.20 Cartridge 10 sampling 580/800ml at 0.51ml/s with 22.3psi vacuum @11:01:35.21 Cartridge 10 sampling 613/800ml at 0.55ml/s with 16.5psi vacuum @11:02:35.22 Cartridge 10 sampling 645/800ml at 0.55ml/s with 20.6psi vacuum @11:03:35.25 Cartridge 10 sampling 677/800ml at 0.52ml/s with 21.3psi vacuum @11:04:35.22 Cartridge 10 sampling 709/800ml at 0.54ml/s with 21.0psi vacuum @11:05:35.21 Cartridge 10 sampling 740/800ml at 0.48ml/s with 20.3psi vacuum @11:06:35.22 Cartridge 10 sampling 771/800ml at 0.53ml/s with 20.7psi vacuum @11:07:33.62 Intake.close @11:07:33.66 Exhaust.close @11:07:34.51 Sampled 800.0ml @11:07:34.64 TV.seek :bypass @11:07:36.27 TV.seek :clear @11:07:39.77 SPR.command "read_sequences" @11:07:39.82 SPR.command "stats_on" @11:07:39.88 SPR.command "start 2 0" @11:07:41.89 Begun log /var/log/esp/spr18jul2711h.spr @11:07:42.55 Handoff.select :waste @11:07:42.71 ArS.seek empty @11:08:00.46 PinPort.seek :cartridge @11:08:06.20 LyS1.seek 1350ul @11:08:12.18 PV.seek :reagent @11:08:13.06 AirS.seek :tVent @11:08:15.16 delay 2 seconds @11:08:17.20 AirS.reconfigure EvacAirS @11:08:17.29 AirS.seek :evac @11:08:39.64 delay 15 seconds @11:08:54.67 AirS.reconfigure AirSconfig @11:08:54.77 AirS.seek :tVent @11:09:08.99 delay 2 seconds @11:09:11.03 AirS.reconfigure EvacAirS @11:09:11.11 AirS.seek :evac @11:09:33.44 delay 15 seconds @11:09:48.47 AirS.reconfigure AirSconfig @11:09:48.56 AirS.seek :tVent @11:10:02.81 delay 2 seconds @11:10:04.84 AirS.reconfigure EvacAirS @11:10:04.95 AirS.seek :evac @11:10:27.28 delay 15 seconds @11:10:42.31 AirS.reconfigure AirSconfig @11:10:42.40 AirS.seek :tVent @11:10:56.65 delay 2 seconds @11:10:58.68 AirS.reconfigure EvacAirS @11:10:58.77 AirS.seek :evac @11:11:21.17 delay 15 seconds @11:11:36.20 AirS.reconfigure AirSconfig @11:11:36.29 AirS.seek :tVent @11:11:50.53 delay 2 seconds @11:11:52.56 AirS.reconfigure EvacAirS @11:11:52.64 AirS.seek :evac @11:12:14.98 delay 15 seconds @11:12:30.01 delay 1:30 @11:14:00.03 AirS.reconfigure AirSconfig @11:14:00.12 AirS.seek :fVent @11:14:01.58 delay 15 seconds @11:14:16.61 LyS1.reconfigure SlowLyS1 @11:14:16.75 LyS1.seek 1050ul @11:14:46.81 delay 5 seconds @11:14:51.84 Handoff.select :blocked @11:14:52.05 LyS1.seek 450ul @11:15:52.05 delay 10 seconds @11:16:02.08 AirS.reconfigure SlowAirS @11:16:02.23 AirS.reconfigure to mix into 1.37806 parts LyS1 @11:16:02.81 AirS.seek evac @11:16:02.85 LyS1.seek 300ul @11:16:18.04 AirS.reconfigure AirSconfig @11:16:18.13 AirS.seek :full @11:16:32.37 LyS1.seek :empty @11:17:02.50 PuckHeater.seek 84.2C @11:17:14.94 -> can @11:18:01.27 PuckHeater.hold 15:00,84.2C @11:32:55.68 LyS1.reconfigure LyS1config @11:32:55.78 LyS1.seek 900ul @11:33:04.15 AirS.seek :tVent @11:33:04.27 delay 2 seconds @11:33:06.31 AirS.reconfigure HalfSpeedAirS @11:33:06.39 AirS.seek :evac @11:33:34.16 delay 15 seconds @11:33:49.20 AirS.reconfigure AirSconfig @11:33:49.29 AirS.seek :tVent @11:34:03.61 delay 2 seconds @11:34:05.66 AirS.reconfigure HalfSpeedAirS @11:34:05.74 AirS.seek :evac @11:34:33.51 delay 15 seconds @11:34:48.54 AirS.reconfigure AirSconfig @11:34:48.63 AirS.seek :tVent @11:35:02.88 delay 2 seconds @11:35:04.91 AirS.reconfigure HalfSpeedAirS @11:35:05.01 AirS.seek :evac @11:35:32.79 delay 15 seconds @11:35:47.82 delay 1:00 @11:36:47.83 Handoff.select :waste @11:36:47.99 delay 15 seconds @11:37:03.02 AirS.seek :fVent @11:37:05.24 delay 10 seconds @11:37:15.27 AirS.reconfigure AirSconfig @11:37:15.35 AirS.seek :evac @11:37:16.81 Lysis Recovered in LyS1 @11:37:16.82 Lysis Complete @11:37:16.87 Handoff.select :waste @11:37:16.91 LyS2.seek :full @11:37:17.04 LyS1.reconfigure DAmixLyS1 @11:37:17.11 LyS2.reconfigure DAmixLyS2 @11:37:17.65 LyS2.seek 1480ul @11:37:17.69 LyS1.seek 650ul @11:38:05.08 delay 5 seconds @11:38:10.10 Handoff.select :SPR @11:38:10.27 SPR.command "start 1 14" @11:38:10.84 Running Experiment-Rep #1 @11:38:10.85 delay 10 seconds @11:38:20.89 SPR.command "start 1 0" @11:38:24.21 Waiting up to 4:00 for SPR to enter state 1 @11:40:28.48 SPR.command "hold_on" @11:40:30.32 LyS2.seek 1130ul @11:40:30.39 LyS1.seek 300ul @11:41:36.66 delay 15 seconds @11:41:51.69 SPR.command "hold_off" @11:41:55.63 Waiting up to 25:00 for SPR to enter state 7 @12:02:00.48 Handoff.select :waste @12:02:00.96 LyS1.reconfigure LyS1config @12:02:01.04 LyS2.reconfigure LyS2config @12:02:01.12 Rep #1 SPR Analysis has Completed @12:02:01.40 Ended log /var/log/esp/spr18jul2711h.spr @12:02:01.42 SPR.command "stats_off" @12:02:01.93 Handoff.select :close @12:02:02.16
PV.seek :locked @12:02:03.18 ArS.seek :clear @12:02:03.37 LyS1.seek :clear @12:02:03.57 LyS2.seek :clear @12:02:03.78 AirS.seek :clear @12:02:20.98
ArS.coast @12:02:21.06 LyS1.coast @12:02:21.14 LyS2.coast @12:02:21.20 AirS.coast @12:02:21.28 TV.coast @12:02:21.35 SP.coast @12:02:21.42 SC.coast @12:02:21.49 PV.coast @12:02:21.57 PinPort.coast @12:02:21.79 Gate.power :motor,:OFF @12:02:21.81 Gate.power :heat,:OFF @12:02:21.85 Gate.power :main,:OFF @12:02:21.88 Gate.power :cartridge,:OFF @12:02:21.92 Gate.power :core,:OFF @12:02:21.96 Gate.power :analytic1,:OFF @12:02:21.99 Gate.power :analytic2,:OFF @12:02:22.02 Gate.power :analytic3,:OFF @12:02:22.06 Safely stopped and ready to power off @12:02:22.27 delay 30 seconds @12:02:52.40 Gate.power :main,:ON,limit to 5000mA @12:02:52.41 Gate.power :analytic1,:ON,limit to 5000mA @12:02:52.45 Gate.power :analytic2,:ON,limit to 5000mA @12:02:52.50 Gate.power :analytic3,:ON,limit to 5000mA @12:02:52.59 Powering core bus @12:02:54.97 SPR Module 1, FW Rev. B, FPGA Rev. C @12:02:55.16
ArS.configure ArSconfig @12:02:55.30 LyS1.configure LyS1config @12:02:55.48 LyS2.configure LyS2config @12:02:55.61 AirS.configure AirSconfig @12:02:55.78 TV.configure TVconfig @12:02:55.95 SP.configure SPconfig @12:02:56.13 SC.configure SCconfig @12:02:56.30 PV.configure PVconfig @12:02:56.47 PinPort.configure PPconfig @12:02:56.63 Sea.configure SPconfig @12:02:56.71 WARNING: Multiple controller firmware versions in use [:carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :toroid0, :ambient0, :carousel0]=>4.46, [:cart]=>4.31 @12:02:56.73 Gate.power :motor,:ON,limit to 5000mA @12:02:57.40 TV.reconfigure TVconfig @12:02:57.49 PV.reconfigure PVconfig @12:02:57.55 LyS2.reconfigure LyS2config @12:02:57.62 AirS.reconfigure AirSconfig @12:02:57.70 LyS1.reconfigure LyS1config @12:02:57.73 ArS.reconfigure ArSconfig @12:02:57.80 PinPort.reconfigure PPconfig @12:02:58.10 PV.home.jog 0 @12:02:58.14 TV.home.jog 0 @12:02:58.23 AirS.home.jog 0 @12:02:58.28 LyS2.home.jog 0 @12:02:58.31 PinPort.home.jog 0 @12:02:58.38 LyS1.home.jog 0 @12:02:58.47 ArS.home.jog 0 @12:02:59.90 PinPort.reconfigure HomePP @12:03:00.00 PV.reconfigure HomePV @12:03:00.06 LyS2.reconfigure HomeSyringe @12:03:00.13 TV.reconfigure HomeTV @12:03:00.19 AirS.reconfigure HomeMagSyringe @12:03:00.23 ArS.reconfigure HomeMagSyringe @12:03:00.36 LyS1.reconfigure HomeSyringe @12:03:00.56 PinPort.jog -2410 @12:03:00.67 PV.jog -1497 @12:03:00.73 LyS2.jog -261 @12:03:00.79 AirS.jog -307 @12:03:00.84 ArS.jog -275 @12:03:00.97 TV.jog -549 @12:03:00.99 LyS1.jog -276 @12:03:01.65 LyS2.setRawPosition! -50 @12:03:01.75 LyS2.seek :home @12:03:01.90 AirS.setRawPosition! -50 @12:03:01.93 LyS1.setRawPosition! -50 @12:03:01.97 ArS.setRawPosition! -50 @12:03:02.13 AirS.seek :home @12:03:02.19 LyS1.seek :home @12:03:02.28 ArS.seek :home @12:03:02.55 PV.setRawPosition! -50 @12:03:02.58 LyS2.reconfigure LyS2config @12:03:02.74 PV.seek :home @12:03:02.91 LyS1.reconfigure LyS1config @12:03:02.94 ArS.reconfigure ArSconfig @12:03:02.98 AirS.reconfigure AirSconfig @12:03:03.28 TV.setRawPosition! 0 @12:03:03.30 PV.reconfigure PVconfig @12:03:03.43 TV.seek :home @12:03:03.59 TV.reconfigure TVconfig @12:03:06.61 PinPort.setRawPosition! 0 @12:03:06.69 PinPort.seek :home @12:03:06.80 PinPort.reconfigure PPconfig @12:03:07.37
TV.seek :clear @12:03:09.01 TV.coast @12:03:09.09 SC.reconfigure HomeSC @12:03:09.18 SC.home.jog 0 @12:03:09.98 SC.reconfigure SCconfig @12:03:10.06 SC.seek 10 @12:03:10.17 Selecting Cartridge 9 @12:03:10.56 SC.seek 9 @12:03:11.12 LyS2.seek :locked @12:03:11.31 PV.seek :locked @12:03:11.50 AirS.seek :locked @12:03:15.62
Powering cartridge bus @12:03:17.76 Gate.setIR 1750,5000,0,6 @12:03:17.88 Gate.setIR 0,5000,0,6 @12:03:17.93 Loaded Archive Cartridge @12:03:17.95 Gate.power :cartridge,:OFF @12:03:18.28 Duration of filtering limited to 1:05:00 @12:03:18.95 Priming sample loop w/100ml, bypass w/10ml @12:03:19.06 SP.setPosition! 0ml @12:03:19.12 Intake.open @12:03:19.16 Exhaust.open @12:03:20.05 Sea @-42.9psia @12:03:20.07 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:03:25.84 Vacuum tare is 21% of full scale @12:03:25.86 PB.open @12:03:25.91 Exhaust.close @12:03:26.82 Sea @-42.9psia @12:03:26.94 SP.reconfigure SPprime @12:03:27.04 SP.seek 10ml,1:21 @12:03:37.94 SP.setPosition! 0ml @12:03:38.02 PB.close @12:03:38.06 Exhaust.open @12:03:38.96 Sea @-42.9psia @12:03:39.08 SP.reconfigure SPprime @12:03:39.17 SP.seek 100ml,8:51 @12:05:25.57 Pressurizing cartridge to 26psi @12:05:25.58 Intake.close @12:05:26.35 SP.setPosition! 0ml @12:05:26.42 PB.open @12:05:27.22 TV.seek :crack @12:05:28.05 TV.seek :clear @12:05:28.89 Intake.open @12:05:30.30 PB.close @12:05:31.06 Exhaust.close @12:05:31.88 Sea @-42.9psia @12:05:31.99 SP.reconfigure SPcharge @12:05:32.08 SP.seek 7.5ml,1:08 @12:05:46.01 Cartridge pressurized with 0.905ml to 26.2psi @12:05:46.31 delay 3 seconds @12:05:49.39 Cartridge at 24.0psi 3 seconds later @12:05:49.52 TV.seek :cartridge @12:05:51.36 TV.seek :clear @12:05:53.20 Sampling 1000.0ml @12:05:53.22 SP.setPosition! 0ml @12:05:53.30 Exhaust.open @12:05:54.13 Sea @-42.9psia @12:05:54.27 SP.reconfigure SPsample @12:05:54.37 SP.seek 1000ml,1:23:53 @12:05:55.30 Cartridge 9 sampling 0/1000ml at 0.15ml/s with 4.4psi vacuum @12:06:55.31 Cartridge 9 sampling 27/1000ml at 0.43ml/s with 20.0psi vacuum @12:07:55.32 Cartridge 9 sampling 53/1000ml at 0.46ml/s with 22.9psi vacuum @12:08:55.30 Cartridge 9 sampling 78/1000ml at 0.38ml/s with 17.1psi vacuum @12:09:55.29 Cartridge 9 sampling 102/1000ml at 0.36ml/s with 22.1psi vacuum @12:10:55.31 Cartridge 9 sampling 125/1000ml at 0.41ml/s with 20.7psi vacuum @12:11:55.31 Cartridge 9 sampling 148/1000ml at 0.36ml/s with 26.9psi vacuum @12:12:55.67 Cartridge 9 sampling 169/1000ml at 0.39ml/s with 21.1psi vacuum @12:13:55.31 Cartridge 9 sampling 190/1000ml at 0.37ml/s with 25.9psi vacuum @12:13:59.66 Session Begun @12:14:01.90 -> showlog @12:14:55.30 Cartridge 9 sampling 210/1000ml at 0.36ml/s with 23.2psi vacuum @12:15:55.31 Cartridge 9 sampling 230/1000ml at 0.28ml/s with 19.6psi vacuum @12:16:55.30 Cartridge 9 sampling 248/1000ml at 0.33ml/s with 21.7psi vacuum @12:17:55.31 Cartridge 9 sampling 266/1000ml at 0.33ml/s with 23.9psi vacuum @12:18:55.31 Cartridge 9 sampling 283/1000ml at 0.30ml/s with 26.3psi vacuum @12:19:55.29 Cartridge 9 sampling 300/1000ml at 0.28ml/s with 26.4psi vacuum @12:20:55.31 Cartridge 9 sampling 316/1000ml at 0.25ml/s with 22.2psi vacuum @12:21:55.31 Cartridge 9 sampling 331/1000ml at 0.26ml/s with 16.5psi vacuum @12:22:55.31 Cartridge 9 sampling 346/1000ml at 0.23ml/s with 25.8psi vacuum @12:23:55.31 Cartridge 9 sampling 361/1000ml at 0.21ml/s with 20.7psi vacuum @12:24:55.30 Cartridge 9 sampling 375/1000ml at 0.24ml/s with 24.4psi vacuum @12:25:55.31 Cartridge 9 sampling 388/1000ml at 0.21ml/s with 24.6psi vacuum @12:26:55.31 Cartridge 9 sampling 401/1000ml at 0.20ml/s with 23.7psi vacuum @12:27:55.31 Cartridge 9 sampling 414/1000ml at 0.23ml/s with 21.7psi vacuum @12:28:55.30 Cartridge 9 sampling 427/1000ml at 0.21ml/s with 23.8psi vacuum @12:29:55.30 Cartridge 9 sampling 439/1000ml at 0.20ml/s with 27.6psi vacuum @12:30:55.31 Cartridge 9 sampling 452/1000ml at 0.20ml/s with 26.5psi vacuum @12:31:55.31 Cartridge 9 sampling 464/1000ml at 0.21ml/s with 16.7psi vacuum @12:32:55.31 Cartridge 9 sampling 477/1000ml at 0.20ml/s with 25.0psi vacuum @12:33:55.30 Cartridge 9 sampling 489/1000ml at 0.20ml/s with 29.0psi vacuum @12:34:55.30 Cartridge 9 sampling 501/1000ml at 0.20ml/s with 25.8psi vacuum @12:35:55.30 Cartridge 9 sampling 513/1000ml at 0.20ml/s with 26.3psi vacuum @12:36:55.31 Cartridge 9 sampling 525/1000ml at 0.20ml/s with 28.5psi vacuum @12:37:55.30 Cartridge 9 sampling 537/1000ml at 0.20ml/s with 29.2psi vacuum @12:38:41.72 Flow averaged 0.28ml/s over 32:42 @12:38:41.77 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @12:38:42.46 Relieved excess vacuum in 6.9 seconds @12:38:42.50 SP.seek 1000ml,38:17 @12:38:55.31 Cartridge 9 sampling 549/1000ml at 0.20ml/s with 29.1psi vacuum @12:39:55.30 Cartridge 9 sampling 561/1000ml at 0.20ml/s with 29.8psi vacuum @12:40:16.33 Flow averaged 0.20ml/s over 1:33 @12:40:16.36 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @12:40:17.06 Relieved excess vacuum in 1.6 seconds @12:40:17.12 SP.seek 1000ml,36:43 @12:40:55.30 Cartridge 9 sampling 573/1000ml at 0.20ml/s with 32.5psi vacuum @12:40:57.19 Flow averaged 0.21ml/s over 39 seconds @12:40:57.21 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @12:40:57.89 Relieved excess vacuum in 2.5 seconds @12:40:57.95 SP.seek 1000ml,36:03 @12:41:03.70 Flow estimate reduced to 0.17ml/s @12:41:03.73 Intake.close @12:41:03.82 Exhaust.close @12:41:04.69 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @12:41:04.85 Sampled 574.7ml @12:41:04.99 TV.seek :bypass @12:41:06.65 TV.seek :clear @12:41:08.68 PV.seek :reagent @12:41:09.56 Sample loop closed. Ready to Deliver Reagents @12:41:09.58 ArS.seek :sealed @12:41:13.96 ArS.seek :evac @12:41:27.94 delay 20 seconds @12:41:47.98 ArS.seek :clear @12:42:05.74 RNAlater soak for 10:00 @12:52:05.78 ArS.seek :tVent @12:52:08.40 delay 1 second @12:52:09.43 ArS.seek :evac @12:52:25.08 delay 10 seconds @12:52:35.11 Sample preserved and evacuated @12:52:35.34
PV.seek :locked @12:52:36.38 ArS.seek :clear @12:52:36.63 LyS2.seek :clear @12:52:36.83 AirS.seek :clear @12:52:54.18
ArS.coast @12:52:54.28 LyS1.coast @12:52:54.36 LyS2.coast @12:52:54.45 AirS.coast @12:52:54.54 TV.coast @12:52:54.62 SP.coast @12:52:54.71 SC.coast @12:52:54.80 PV.coast @12:52:54.89 PinPort.coast @12:52:55.13 Gate.power :motor,:OFF @12:52:55.15 Gate.power :main,:OFF @12:52:55.21 Gate.power :core,:OFF @12:52:55.28 Gate.power :analytic1,:OFF @12:52:55.31 Gate.power :analytic2,:OFF @12:52:55.36 Gate.power :analytic3,:OFF @12:52:55.40 Safely stopped and ready to power off @12:52:55.64 delay 30 seconds @12:53:25.77 Gate.power :main,:ON,limit to 5000mA @12:53:25.79 Gate.power :analytic1,:ON,limit to 5000mA @12:53:25.85 Gate.power :analytic2,:ON,limit to 5000mA @12:53:25.90 Gate.power :analytic3,:ON,limit to 5000mA @12:53:26.02 Powering core bus @12:53:28.41 SPR Module 1, FW Rev. B, FPGA Rev. C @12:53:28.60
ArS.configure ArSconfig @12:53:28.75 LyS1.configure LyS1config @12:53:28.93 LyS2.configure LyS2config @12:53:29.08 AirS.configure AirSconfig @12:53:29.26 TV.configure TVconfig @12:53:29.44 SP.configure SPconfig @12:53:29.62 SC.configure SCconfig @12:53:29.80 PV.configure PVconfig @12:53:29.98 PinPort.configure PPconfig @12:53:30.15 Sea.configure SPconfig @12:53:30.25 All elf controllers running firmware version 4.46 @12:53:30.28 Gate.power :motor,:ON,limit to 5000mA @12:53:31.42 PinPort.reconfigure PPconfig @12:53:31.48 PV.reconfigure PVconfig @12:53:31.52 TV.reconfigure TVconfig @12:53:31.63 AirS.reconfigure AirSconfig @12:53:31.67 LyS1.reconfigure LyS1config @12:53:31.73 ArS.reconfigure ArSconfig @12:53:31.85 LyS2.reconfigure LyS2config @12:53:32.16 PV.home.jog 0 @12:53:32.22 PinPort.home.jog 0 @12:53:32.26 TV.home.jog 0 @12:53:32.34 LyS1.home.jog 0 @12:53:32.40 AirS.home.jog 0 @12:53:32.46 LyS2.home.jog 0 @12:53:32.50 ArS.home.jog 0 @12:53:33.55 PinPort.reconfigure HomePP @12:53:33.62 PV.reconfigure HomePV @12:53:33.66 AirS.reconfigure HomeMagSyringe @12:53:33.76 LyS1.reconfigure HomeSyringe @12:53:33.82 ArS.reconfigure HomeMagSyringe @12:53:33.93 LyS2.reconfigure HomeSyringe @12:53:34.00 TV.reconfigure HomeTV @12:53:34.30 PV.jog -1495 @12:53:34.41 PinPort.jog -109 @12:53:34.46 LyS1.jog -275 @12:53:34.52 AirS.jog -296 @12:53:34.65 LyS2.jog -261 @12:53:34.70 ArS.jog -273 @12:53:34.76 TV.jog -548 @12:53:35.20 PinPort.setRawPosition! 0 @12:53:35.29 PinPort.seek :home @12:53:35.45 PinPort.reconfigure PPconfig @12:53:35.50 LyS1.setRawPosition! -50 @12:53:35.69 LyS1.seek :home @12:53:35.87 AirS.setRawPosition! -50 @12:53:35.91 ArS.setRawPosition! -50 @12:53:35.96 PV.setRawPosition! -50 @12:53:36.02 LyS2.setRawPosition! -50 @12:53:36.21 AirS.seek :home @12:53:36.28 ArS.seek :home @12:53:36.41 PV.seek :home @12:53:36.54 LyS2.seek :home @12:53:36.86 LyS1.reconfigure LyS1config @12:53:36.91 AirS.reconfigure AirSconfig @12:53:37.19 PV.reconfigure PVconfig @12:53:37.23 TV.setRawPosition! 0 @12:53:37.31 LyS2.reconfigure LyS2config @12:53:37.35 ArS.reconfigure ArSconfig @12:53:37.65 TV.seek :home @12:53:37.81 TV.reconfigure TVconfig @12:53:38.14
TV.seek :clear @12:53:39.79 TV.coast @12:53:39.88 SC.reconfigure HomeSC @12:53:39.99 SC.home.jog 0 @12:53:40.80 SC.reconfigure SCconfig @12:53:41.24 SC.seek 9 @12:53:41.39 Selecting Cartridge 8 @12:53:41.80 SC.seek 8 @12:53:42.40 LyS2.seek :locked @12:53:42.60 PV.seek :locked @12:53:42.83 AirS.seek :locked @12:53:46.96
Powering cartridge bus @12:53:49.10 Gate.setIR 1750,5000,0,6 @12:53:49.24 Gate.setIR 0,5000,0,6 @12:53:49.33 Loaded Lyse-n-Go Cartridge @12:53:49.34 PuckHeater.configure HeatConfig @12:53:49.46 Gate.power :heat,:ON,limit to 5000mA @12:53:49.78 Duration of filtering limited to 1:05:00 @12:53:50.13 Priming sample loop w/100ml, bypass w/10ml @12:53:50.26 SP.setPosition! 0ml @12:53:50.34 Intake.open @12:53:50.38 Exhaust.open @12:53:51.31 Sea @-42.9psia @12:53:51.34 Waiting 4 - 300s for filter pressure to stabilize +/-0.5psi @12:53:57.11 Vacuum tare is 21% of full scale @12:53:57.14 PB.open @12:53:57.20 Exhaust.close @12:53:58.14 Sea @-42.9psia @12:53:58.28 SP.reconfigure SPprime @12:53:58.39 SP.seek 10ml,1:21 @12:54:09.31 SP.setPosition! 0ml @12:54:09.40 PB.close @12:54:09.47 Exhaust.open @12:54:10.39 Sea @-42.9psia @12:54:10.52 SP.reconfigure SPprime @12:54:10.64 SP.seek 100ml,8:51 @12:55:56.94 Pressurizing cartridge to 26psi @12:55:56.95 Intake.close @12:55:57.79 SP.setPosition! 0ml @12:55:57.87 PB.open @12:55:58.72 TV.seek :crack @12:55:59.54 TV.seek :clear @12:56:00.39 Intake.open @12:56:01.87 PB.close @12:56:02.65 Exhaust.close @12:56:03.46 Sea @-42.9psia @12:56:03.61 SP.reconfigure SPcharge @12:56:03.71 SP.seek 7.5ml,1:08 @12:56:18.09 Cartridge pressurized with 0.961ml to 26.2psi @12:56:18.40 delay 3 seconds @12:56:21.50 Cartridge at 21.6psi 3 seconds later @12:56:21.99 TV.seek :cartridge @12:56:23.85 TV.seek :clear @12:56:25.72 Sampling 800.0ml @12:56:25.74 SP.setPosition! 0ml @12:56:25.86 Exhaust.open @12:56:26.69 Sea @-42.9psia @12:56:26.84 SP.reconfigure SPsample @12:56:26.95 SP.seek 800ml,1:07:12 @12:56:27.85 Cartridge 8 sampling 0/800ml at 0.15ml/s with 3.4psi vacuum @12:57:27.86 Cartridge 8 sampling 36/800ml at 0.60ml/s with 22.2psi vacuum @12:58:27.86 Cartridge 8 sampling 72/800ml at 0.61ml/s with 23.3psi vacuum @12:59:27.86 Cartridge 8 sampling 108/800ml at 0.59ml/s with 24.9psi vacuum @13:00:27.87 Cartridge 8 sampling 144/800ml at 0.59ml/s with 23.6psi vacuum @13:01:27.86 Cartridge 8 sampling 178/800ml at 0.58ml/s with 22.8psi vacuum @13:02:27.86 Cartridge 8 sampling 213/800ml at 0.56ml/s with 22.1psi vacuum @13:03:27.86 Cartridge 8 sampling 247/800ml at 0.57ml/s with 23.1psi vacuum @13:04:27.85 Cartridge 8 sampling 281/800ml at 0.56ml/s with 21.1psi vacuum @13:05:27.85 Cartridge 8 sampling 316/800ml at 0.57ml/s with 23.0psi vacuum @13:06:27.86 Cartridge 8 sampling 350/800ml at 0.56ml/s with 25.0psi vacuum @13:07:27.86 Cartridge 8 sampling 385/800ml at 0.58ml/s with 21.7psi vacuum @13:07:34.02 Session Ended @13:08:27.85 Cartridge 8 sampling 419/800ml at 0.60ml/s with 23.4psi vacuum @13:08:52.22 Session Begun @13:08:56.28 -> showlog 10 @13:09:27.85 Cartridge 8 sampling 454/800ml at 0.58ml/s with 25.6psi vacuum @13:10:27.85 Cartridge 8 sampling 488/800ml at 0.55ml/s with 21.1psi vacuum @13:11:27.86 Cartridge 8 sampling 522/800ml at 0.58ml/s with 20.2psi vacuum @13:12:27.86 Cartridge 8 sampling 556/800ml at 0.55ml/s with 27.3psi vacuum @13:13:27.86 Cartridge 8 sampling 589/800ml at 0.53ml/s with 18.3psi vacuum @13:14:27.86 Cartridge 8 sampling 621/800ml at 0.55ml/s with 16.6psi vacuum @13:15:27.85 Cartridge 8 sampling 653/800ml at 0.50ml/s with 26.0psi vacuum @13:16:27.86 Cartridge 8 sampling 684/800ml at 0.55ml/s with 24.8psi vacuum @13:17:27.87 Cartridge 8 sampling 715/800ml at 0.48ml/s with 25.6psi vacuum @13:18:27.86 Cartridge 8 sampling 745/800ml at 0.52ml/s with 19.3psi vacuum @13:19:27.86 Cartridge 8 sampling 775/800ml at 0.52ml/s with 20.7psi vacuum @13:20:23.11 Intake.close @13:20:23.17 Exhaust.close @13:20:24.03 Sampled 800.0ml @13:20:24.17 TV.seek :bypass @13:20:25.84 TV.seek :clear @13:20:29.37 SPR.command "read_sequences" @13:20:29.45 SPR.command "stats_on" @13:20:29.52 SPR.command "start 2 0" @13:20:31.52 Begun log /var/log/esp/spr18jul2713h.spr AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AMspr (try 1 of 3) @13:20:34.69 Handoff.select :waste @13:20:34.87 ArS.seek empty @13:20:52.65 PinPort.seek :cartridge @13:20:58.43 LyS1.seek 1350ul @13:21:04.43 PV.seek :reagent @13:21:05.32 AirS.seek :tVent @13:21:07.44 delay 2 seconds @13:21:09.47 AirS.reconfigure EvacAirS @13:21:09.58 AirS.seek :evac @13:21:31.96 delay 15 seconds @13:21:46.99 AirS.reconfigure AirSconfig @13:21:47.10 AirS.seek :tVent @13:22:01.35 delay 2 seconds @13:22:03.38 AirS.reconfigure EvacAirS @13:22:03.49 AirS.seek :evac @13:22:25.85 delay 15 seconds @13:22:40.88 AirS.reconfigure AirSconfig @13:22:40.98 AirS.seek :tVent @13:22:55.25 delay 2 seconds @13:22:57.28 AirS.reconfigure EvacAirS @13:22:57.38 AirS.seek :evac @13:23:19.72 delay 15 seconds @13:23:34.75 AirS.reconfigure AirSconfig @13:23:34.87 AirS.seek :tVent @13:23:49.12 delay 2 seconds @13:23:51.15 AirS.reconfigure EvacAirS @13:23:51.26 AirS.seek :evac @13:24:13.60 delay 15 seconds @13:24:28.63 AirS.reconfigure AirSconfig @13:24:28.74 AirS.seek :tVent @13:24:42.99 delay 2 seconds @13:24:45.02 AirS.reconfigure EvacAirS @13:24:45.12 AirS.seek :evac @13:25:07.47 delay 15 seconds @13:25:22.50 delay 1:30 @13:26:52.53 AirS.reconfigure AirSconfig @13:26:52.69 AirS.seek :fVent @13:26:54.18 delay 15 seconds @13:27:09.21 LyS1.reconfigure SlowLyS1 @13:27:09.38 LyS1.seek 1050ul @13:27:39.44 delay 5 seconds @13:27:44.46 Handoff.select :blocked @13:27:44.69 LyS1.seek 450ul @13:28:44.68 delay 10 seconds @13:28:54.71 AirS.reconfigure SlowAirS @13:28:54.88 AirS.reconfigure to mix into 1.37806 parts LyS1 @13:28:55.49 AirS.seek evac @13:28:55.54 LyS1.seek 300ul @13:29:10.74 AirS.reconfigure AirSconfig @13:29:10.85 AirS.seek :full @13:29:25.11 LyS1.seek :empty @13:29:55.27 PuckHeater.seek 84.1C @13:31:00.08 PuckHeater.hold 15:00,84.1C @13:41:01.52 -> Heat @13:41:07.86 -> Heat @13:41:10.81 -> Heat @13:41:19.68 -> Heat @13:41:22.88 -> Heat @13:41:25.63 -> Heat @13:41:28.90 -> Heat @13:46:00.19 LyS1.reconfigure LyS1config @13:46:00.32 LyS1.seek 900ul @13:46:08.71 AirS.seek :tVent @13:46:08.86 delay 2 seconds @13:46:10.89 AirS.reconfigure HalfSpeedAirS @13:46:11.00 AirS.seek :evac @13:46:38.75 delay 15 seconds @13:46:53.78 AirS.reconfigure AirSconfig @13:46:53.89 AirS.seek :tVent @13:47:08.14 delay 2 seconds @13:47:10.17 AirS.reconfigure HalfSpeedAirS @13:47:10.29 AirS.seek :evac @13:47:38.08 delay 15 seconds @13:47:53.11 AirS.reconfigure AirSconfig @13:47:53.22 AirS.seek :tVent @13:48:07.48 delay 2 seconds @13:48:09.51 AirS.reconfigure HalfSpeedAirS @13:48:09.60 AirS.seek :evac @13:48:37.38 delay 15 seconds @13:48:52.41 delay 1:00 @13:49:52.44 Handoff.select :waste @13:49:52.62 delay 15 seconds @13:50:07.65 AirS.seek :fVent @13:50:09.87 delay 10 seconds @13:50:19.90 AirS.reconfigure AirSconfig @13:50:20.00 AirS.seek :evac @13:50:21.48 Lysis Recovered in LyS1 @13:50:21.49 Lysis Complete @13:50:21.55 Handoff.select :waste @13:50:21.63 LyS2.seek :full @13:50:21.76 LyS1.reconfigure DAmixLyS1 @13:50:21.85 LyS2.reconfigure DAmixLyS2 @13:50:22.44 LyS2.seek 1480ul @13:50:22.50 LyS1.seek 650ul @13:51:09.87 delay 5 seconds @13:51:14.89 Handoff.select :SPR @13:51:15.05 SPR.command "start 1 14" @13:51:15.69 Running Experiment-Rep #1 @13:51:15.71 delay 10 seconds @13:51:25.77 SPR.command "start 1 0" @13:51:29.05 Waiting up to 4:00 for SPR to enter state 1 @13:53:33.34 SPR.command "hold_on" @13:53:35.69 LyS2.seek 1130ul @13:53:35.73 LyS1.seek 300ul @13:54:41.98 delay 15 seconds @13:54:57.00 SPR.command "hold_off" @13:54:59.98 Waiting up to 25:00 for SPR to enter state 7 @14:15:03.50 Handoff.select :waste @14:15:03.67 LyS1.reconfigure LyS1config @14:15:03.76 LyS2.reconfigure LyS2config @14:15:03.85 Rep #1 SPR Analysis has Completed @14:15:04.36 Ended log /var/log/esp/spr18jul2713h.spr @14:15:04.38 SPR.command "stats_off" @14:15:04.91 Handoff.select :close @14:15:05.15
PV.seek :locked @14:15:06.22 ArS.seek :clear @14:15:06.42 LyS1.seek :clear @14:15:06.66 LyS2.seek :clear @14:15:06.88 AirS.seek :clear @14:15:24.04
ArS.coast @14:15:24.13 LyS1.coast @14:15:24.21 LyS2.coast @14:15:24.30 AirS.coast @14:15:24.39 TV.coast @14:15:24.48 SP.coast @14:15:24.57 SC.coast @14:15:24.65 PV.coast @14:15:24.74 PinPort.coast @14:15:24.97 Gate.power :motor,:OFF @14:15:24.99 Gate.power :heat,:OFF @14:15:25.04 Gate.power :main,:OFF @14:15:25.10 Gate.power :cartridge,:OFF @14:15:25.14 Gate.power :core,:OFF @14:15:25.18 Gate.power :analytic1,:OFF @14:15:25.23 Gate.power :analytic2,:OFF @14:15:25.28 Gate.power :analytic3,:OFF @14:15:25.32 Safely stopped and ready to power off @14:15:25.54 delay 30 seconds @14:15:31.34 -> a=[] @14:15:33.83 -> a<<7 @14:15:55.58
No dry cartridges remain @14:15:55.70 Session Ended @14:15:55.74 Session Ended