@21:06:37.87EDT15-Aug-19
>BREAK< ESP3 Gateway v0.99 for core bus @21:06:37.88 >BREAK< ESP3 Gateway v0.99 for cartridge bus @21:06:39.46 Configuration for honu by brent@mbari.org 7/26/19 /home/esp/esp2/type/auv/honu/configure.rb @21:06:42.18 Polling Can every 5:00 @21:06:42.48 Clients may connect on TCP port 9999 @21:06:42.49 Connecting to 10.89.5.2:9999 @21:06:42.52 Errno::ECONNREFUSED in MAIN -- Could not connect to initial client because Connection refused - connect(2) @21:06:42.53 Waiting for espclients... @21:06:50.80 Session Begun @21:08:10.85 -> SC @21:08:10.87 I2C::NodeOffline in preston -- carousel0 microcontroller never signed on while reading Storage Carousel (SC)'s status @21:08:40.96 -> ESP.ready! @21:08:41.08 Gate.power :main,:ON,limit to 5000mA @21:08:41.09 Gate.power :analytic1,:ON,limit to 5000mA @21:08:41.12 Gate.power :analytic2,:ON,limit to 5000mA @21:08:41.14 Gate.power :analytic3,:ON,limit to 5000mA @21:08:41.28 Powering core bus @21:08:43.55 SPR Module 1, FW Rev. B, FPGA Rev. C @21:08:43.68 Archive.configure :default @21:08:43.81 Air.configure :default @21:08:43.91 Lysis1.configure :default @21:08:44.01 Lysis2.configure :default @21:08:44.13 Plunge.configure :default @21:08:44.25 SamplePump.configure :default @21:08:44.38 ExtraPump.configure :default @21:08:44.50 Toroid.configure :default @21:08:44.62 Carousel.configure :default @21:08:44.74 Pressure.configure :default @21:08:44.84 PinPort.configure :default @21:08:44.90 All elf controllers running firmware version 4.58 @21:08:44.93 Gate.power :motor,:ON,limit to 5000mA @21:08:45.41 Toroid.reconfigure :default @21:08:45.46 Plunge.reconfigure :default @21:08:45.49 Lysis1.reconfigure :default @21:08:45.53 Archive.reconfigure :default @21:08:45.57 PinPort.reconfigure :default @21:08:45.61 Lysis2.reconfigure :default @21:08:45.69 Air.reconfigure :default @21:08:45.90 Air.home.jog 0 @21:08:45.96 Lysis1.home.jog 0 @21:08:46.03 Archive.home.jog 0 @21:08:46.08 PinPort.home.jog 0 @21:08:46.14 Lysis2.home.jog 0 @21:08:46.20 Plunge.home.jog 0 @21:08:46.26 Toroid.home.jog 0 @21:08:46.80 Toroid.reconfigure :homing @21:08:46.82 Plunge.reconfigure :homing @21:08:46.85 Air.reconfigure :homing @21:08:46.89 Archive.reconfigure :homing @21:08:46.93 PinPort.reconfigure :homing @21:08:46.98 Lysis1.reconfigure :homing @21:08:47.02 Lysis2.reconfigure :homing @21:08:47.26 Lysis2.jog 1422 @21:08:47.32 Archive.jog 1383 @21:08:47.38 Plunge.jog 1551 @21:08:47.44 Lysis1.jog 1399 @21:08:47.49 Toroid.jog 632 @21:08:47.56 PinPort.jog -105 @21:08:47.62 Air.jog 1408 @21:08:47.78 PinPort.setRawPosition! 0 @21:08:47.83 PinPort.seek :home @21:08:47.92 PinPort.reconfigure :default @21:08:48.43 Lysis2.setRawPosition! 50 @21:08:48.49 Lysis2.seek :home @21:08:48.58 Archive.setRawPosition! 50 @21:08:48.63 Archive.seek :home @21:08:48.72 Plunge.setRawPosition! 50 @21:08:48.73 Lysis1.setRawPosition! 50 @21:08:48.82 Plunge.seek :home @21:08:48.86 Lysis1.seek :home @21:08:49.01 Lysis2.reconfigure :default @21:08:49.04 Air.setRawPosition! 50 @21:08:49.06 Archive.reconfigure :default @21:08:49.21 Air.seek :home @21:08:49.35 Plunge.reconfigure :default @21:08:49.37 Lysis1.reconfigure :default @21:08:49.54 Air.reconfigure :default @21:08:49.64 Toroid.setRawPosition! 0 @21:08:49.69 Toroid.seek :home @21:08:49.78 Toroid.reconfigure :default @21:08:49.92 Toroid.seek :clear @21:08:51.55 Carousel.reconfigure :default @21:08:51.63 Carousel.home.jog 0 @21:08:52.12 Carousel.seek 58 @21:09:11.09 -> SC @21:09:15.41 -> to 58 @21:09:15.65 Carousel.seek 58 @21:09:15.82 Lysis1.seek :locked @21:09:19.95 Plunge.seek :locked @21:09:20.08 Air.seek :locked @21:09:24.20 Powering cartridge bus @21:09:26.33 Gate.setIR 1750,5000,0,6 @21:09:26.46 Gate.setIR 0,5000,0,6 @21:09:26.52 Loaded Lyse-n-Go Cartridge @21:09:26.53 PuckHeater.configure HeatConfig @21:09:26.58 Gate.power :heat,:ON,limit to 5000mA @21:11:15.89 -> Handoff @21:11:41.73 -> PP.cartridge @21:11:41.75 PinPort.seek :cartridge @21:13:04.15 -> LyS1.to 80 @21:13:04.17 Lysis1.seek 80ul @21:13:30.25 -> lyS2.to 1700 @21:13:30.27 NoMethodError in preston -- private method `to' called for :lyS2:Symbol @21:14:00.38 -> LyS2.to 1700 @21:14:00.40 Lysis2.seek 1700ul @21:14:55.42 -> Syringe.PSI @21:15:30.80 -> AirS.fvent @21:15:30.83 Air.seek :fvent @21:16:01.13 -> Syringe.PSI @21:17:31.04 -> AirS.full @21:17:31.06 Air.seek :full @21:18:01.03 -> Syringe.PSI @21:19:37.46 -> AirS.evac; delay 2; Syringe.PSI; AirS.full @21:19:37.49 Air.seek :evac @21:19:51.71 delay 2 seconds @21:19:53.78 Air.seek :full @21:20:01.68 Air.hold @21:20:01.72 Errno::EPIPE in preston aborter-'s_reader -- Broken pipe @21:20:01.75 Errno::EPIPE in preston's_reader -- Broken pipe @21:20:01.76 Session Ended @21:20:07.97 I2C::UnexpectedReply in core -- I2C::Servo::AbsMove3Reply[5D:43->20] @21:20:37.46 Session Begun @21:21:01.90 -> Syringe.PSI @21:21:32.02 -> AirS @21:21:53.08 -> Cartridge.sampler.recoverLysate @21:21:53.09 Handoff.select :blocked @21:21:53.28 Lysis1.reconfigure :halfSpeed @21:21:53.35 Lysis1.seek 280ul @21:21:57.12 delay 1 second @21:21:58.14 Lysis1.seek :empty @21:22:03.36 Initial AirS pressure @27.6psi @21:22:03.43 Lysis1.reconfigure :extract @21:22:03.53 Lysis1.seek 900ul,10:00 @21:22:04.68 Air pumping 10 strokes to reach 24psi @21:22:04.69 Air stroke #1 of 10 @21:22:04.70 Air.reconfigure :pressurize @21:22:04.79 Air.seek :pressurized @21:22:17.62 20.3psi after pressurize @21:22:17.64 Air.reconfigure :compress @21:22:17.72 Air.seek :evac @21:22:20.38 19.4psi after compress @21:22:34.23 18.7psi after compress (stable) @21:22:34.25 Air.reconfigure :gulp @21:22:34.32 Air.seek :tVent @21:22:44.20 18.7psi after gulp @21:22:44.22 Air stroke #2 of 10 @21:22:44.24 Air.reconfigure :pressurize @21:22:44.32 Air.seek :pressurized @21:22:57.14 19.7psi after pressurize @21:22:57.16 Air.reconfigure :compress @21:22:57.24 Air.seek :evac @21:22:59.90 21.2psi after compress @21:23:00.52 Reached 24.5 of 24.0psi after pumping 4672ul (3.00 strokes) @21:23:02.37 Lysis1 at 900ul @21.0psi @21:23:02.39 Lysis1.reconfigure :default @21:23:02.47 Lysis1.seek 925ul @21:23:03.18 Syringe::Overpressure in preston -- 21.9psi after recovery pull @21:23:26.27 -> AirS.fVent @21:23:26.29 Air.reconfigure :default @21:23:26.41 Air.seek :fVent @21:23:51.21 -> Handoff.waste @21:23:51.22 Handoff.select :waste @21:24:21.21 -> Syringe.psi @21:24:51.23 -> LyS1 @21:24:55.69 -> AirS.full @21:24:55.71 Air.seek :full @21:25:11.30 -> LyS2 @21:25:27.60 -> HaNDOFF @21:25:27.61 NameError in preston -- uninitialized constant HaNDOFF @21:25:31.38 -> HANDOFF @21:25:31.39 NameError in preston -- uninitialized constant HANDOFF @21:25:51.81 -> Handoff @21:26:26.09 -> microSPRmanual @21:26:27.71 SPR.command "read_sequences" @21:26:28.46 SPR.command "stats_on" @21:26:28.50 SPR.command "start 0 1" @21:26:30.51 Begun log /var/log/esp/spr19aug1521h.spr @21:26:31.31 Wait 10 minutes for baseline stabilization @21:26:31.32 delay 10:00 @21:36:31.35 Handoff.select :waste @21:36:31.37 Lysis1.reconfigure :DAmix @21:36:31.51 Lysis2.reconfigure :DAmix @21:36:31.90 Lysis1.seek 675ul @21:36:31.98 Lysis2.seek 1450ul @21:37:07.03 delay 5 seconds @21:37:12.05 Handoff.select SPR @21:37:12.17 SPR.command "start 2 14" @21:37:13.63 delay 10 seconds @21:37:23.65 SPR.command "start 2 0" @21:37:26.89 Waiting up to 11:00 for SPR to enter state 1 @21:45:58.96 SPR.command "hold_on" @21:46:00.35 SPR Dilution 1 of 1 -- Running Experiment 2 @21:46:00.84 Lysis1.seek 375ul @21:46:00.88 Lysis2.seek 1150ul @21:46:42.92 volL1 = 300.0 and volL2 = 300.0 @21:46:42.93 delay 15 seconds @21:46:57.96 SPR.command "hold_off" @21:47:01.00 Waiting up to 25:00 for SPR to enter state 7 @21:58:37.52 SPR Analysis has Completed @21:58:37.53 Handoff.select :waste @21:58:38.45 Ended log /var/log/esp/spr19aug1521h.spr @21:58:38.46 SPR.command "stats_off" @21:59:26.98 -> exitexit @21:59:29.35 -> exit @21:59:29.36 Session Ended @22:11:54.03 Session Begun @22:12:18.19 -> slots @22:13:25.25 -> Cartridge.release 57..3 @22:13:48.59 -> s @22:14:18.56 -> slots @22:14:45.95 -> exit @22:14:45.96 Session Ended