@15:21:58.99PDT26-May-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:21:59.03 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:22:02.25 Configuration for honu by brent@mbari.org 6/26/23 /home/esp/esp2/type/auv/honu/configure.rb @15:22:02.88 Sea pressure (Abs) tare is 6% of full scale @15:22:04.72 Polling Can every 2:00 @15:22:04.78 Waiting for espclients... @15:22:04.96 Session Begun @15:23:05.48 -> class PumpSwitch < Switch @15:23:05.51 SyntaxError in brent -- compile error brent:1: syntax error, unexpected $end, expecting '\n' or ';' class PumpSwitch < Switch ^ @15:23:05.65 -> class PumpControl < Solenoid::NonLatching @15:23:05.67 SyntaxError in brent -- compile error brent:2: syntax error, unexpected $end, expecting '\n' or ';' class PumpControl < Solenoid::NonLatching ^ @15:23:05.81 -> def initialize @15:23:05.83 SyntaxError in brent -- compile error brent:3: syntax error, unexpected $end, expecting '\n' or ';' def initialize ^ @15:23:06.05 -> super 0=>[:off,:stop,:home], -1=>[:on, :start] @15:23:06.08 NoMethodError in brent -- undefined method `topLevelBinding' for CmdServer::Client:Class @15:23:06.18 -> end @15:23:06.20 SyntaxError in brent -- compile error brent:5: syntax error, unexpected kEND @15:23:06.30 -> end @15:23:06.32 SyntaxError in brent -- compile error brent:6: syntax error, unexpected kEND @15:23:48.18 -> class PumpSwitch < Switch class PumpControl < Solenoid::NonLatching def initialize super 0=>[:off,:stop,:home], -1=>[:on, :start] end @15:23:48.22 SyntaxError in brent -- compile error brent:15: syntax error, unexpected $end, expecting kEND @15:24:06.53 -> class PumpSwitch < Switch class PumpControl < Solenoid::NonLatching def initialize super 0=>[:off,:stop,:home], -1=>[:on, :start] end end @15:24:06.61 SyntaxError in brent -- compile error brent:22: syntax error, unexpected $end, expecting kEND @15:24:41.01 -> class PumpSwitch < Switch class PumpControl < Solenoid::NonLatching def initialize super 0=>[:off,:stop,:home], -1=>[:on, :start] end end end @15:24:41.05 NameError in brent -- uninitialized constant Switch @15:25:17.98 -> class Switch < Solenoid; end @15:25:37.54 -> class PumpSwitch < Switch class PumpControl < Solenoid::NonLatching def initialize super 0=>[:off,:stop,:home], -1=>[:on, :start] end end end :initialize @15:28:25.38 -> class PumpSwitch; :Macerator.denotes {new "Macerating External Sample Pump", HandoffValves, 0, PumpControl.new; end @15:28:25.41 SyntaxError in brent -- compile error brent:33: syntax error, unexpected kEND, expecting '}' ...alves, 0, PumpControl.new; end ^ @15:28:41.70 -> class PumpSwitch; :Macerator.denotes {new "Macerating External Sample Pump", HandoffValves, 0, PumpControl.new}; end Macerating External Sample Pump PumpSwitch off @15:28:45.55 -> ESP @15:28:50.22 -> ESP.configure @15:28:50.39 Gate.power :analytic1,:ON,limit to 5000mA @15:28:50.41 Gate.power :analytic2,:ON,limit to 5000mA @15:28:50.44 Gate.power :analytic3,:ON,limit to 5000mA @15:28:51.20 Archive.configure :default @15:28:51.39 Air.configure :default @15:28:51.92 Lysis1.configure :default @15:28:52.07 Lysis2.configure :default @15:28:52.26 Plunge.configure :default @15:28:52.47 SamplePump.configure :default @15:28:52.94 Toroid.configure :default @15:28:53.04 Toroid.hold @15:28:53.14 Toroid.configure :default @15:28:53.32 Carousel.configure :default @15:28:53.42 Carousel.hold @15:28:53.51 Carousel.configure :default @15:28:53.70 Pressure.configure :default @15:28:53.87 ExtraPump.configure :default @15:28:54.04 PinPort.configure :default @15:28:54.13 All elf controllers running firmware version 4.83 @15:28:56.02 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @15:28:56.05 AnalyticModule::Unknown in brent -- SPR @15:28:58.27 -> ESP Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 44 Pressure = Absolute Pressure (Leak) Sensor @8psia PinPort = Handoff Pin Port (PP) clear Macerator = Macerating External Sample Pump PumpSwitch off @15:29:08.05 -> Macerator.on @15:29:08.08 Macerator.select :on Macerating External Sample Pump PumpSwitch on @15:29:13.03 -> can Can@15:29:13, 20.6C, 49% humidity, 14.7psia, 14.863V, 0.227A, 0.095Ah, 3.37W @15:29:17.81 -> Macerator.off @15:29:17.82 Macerator.select :off Macerating External Sample Pump PumpSwitch off @15:38:21.35 -> Power {analytic1: true, analytic2: true, analytic3: true, cartridge: false, core: true, heat: false, main: true, motor: true} @15:38:39.80 -> Power.analytic3 on @15:38:39.82 Gate.power :analytic3,:ON true @15:38:46.13 -> Power.status {analytic1: ON with current limited to 5000mA, analytic2: ON with current limited to 5000mA, analytic3: ON with current limited to 5000mA, cartridge: off, core: ON, heat: off with current limited to 0mA, main: ON with current limited to 5000mA, motor: ON with current limited to 5000mA} @15:39:10.91 -> Power analytic1,:off @15:39:10.94 NoMethodError in brent -- undefined method `Power' for CmdServer::Client:Class @15:39:29.02 -> Power :analytic1, off @15:39:29.04 NoMethodError in brent -- undefined method `Power' for CmdServer::Client:Class @15:39:41.75 -> exit @15:39:41.77 Session Ended @15:39:45.51 Session Begun @15:40:01.95 -> Gate.power :analytic1,:off @15:40:01.97 Gate.power :analytic1,:OFF false @15:40:06.02 -> Gate.power :analytic2,:off @15:40:06.03 Gate.power :analytic2,:OFF false @15:41:20.54 -> Power.analytic3 on @15:41:20.57 Gate.power :analytic3,:ON true @15:50:08.07 Session Begun @15:50:14.87 -> Power.analytic3 off @15:50:14.90 Gate.power :analytic3,:OFF false @15:50:26.53 -> Power.analytic3 on @15:50:26.55 Gate.power :analytic3,:ON true @15:50:29.03 -> Power.analytic3 off @15:50:29.05 Gate.power :analytic3,:OFF false nohup: procesAll: No such file or directory @15:35:20.69PDT24-Jul-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @15:35:22.32 >BREAK< ESP3 Gateway v1.42 for core bus @15:35:22.34 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:35:25.82 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @15:35:26.44 Sea pressure (Abs) tare is 6% of full scale @15:35:28.38 Polling Can every 2:00 @15:35:28.43 Loading /home/esp/esp2/bin/processAll mission @15:35:28.69 Sea pressure (Abs) tare is 6% of full scale @15:35:28.71 Sampling default goal volumes @15:35:28.73 Options: {extraDilutions: [1]} Cartridge group: standard @15:35:28.88 Gate.power :sampler,:OFF,limit to 3500mA @15:35:28.93 Gate.power :analytic1,:ON,limit to 5000mA @15:35:28.94 Gate.power :analytic2,:ON,limit to 5000mA @15:35:29.50 SPR Module 1, FW Rev. B, FPGA Rev. C @15:35:30.04
Archive.configure :default @15:35:30.20 Air.configure :default @15:35:30.33 Lysis1.configure :default @15:35:30.47 Lysis2.configure :default @15:35:30.65 Plunge.configure :default @15:35:30.81 SamplePump.configure :default @15:35:30.97 Toroid.configure :default @15:35:31.03 Toroid.hold @15:35:31.10 Toroid.configure :default @15:35:31.27 Carousel.configure :default @15:35:31.33 Carousel.hold @15:35:31.39 Carousel.configure :default @15:35:31.54 Pressure.configure :default @15:35:31.70 ExtraPump.configure :default @15:35:31.82 PinPort.configure :default @15:35:31.88 All elf controllers running firmware version 4.84 @15:35:32.75 Toroid.seek :clear @15:35:32.83 Carousel.in :default @15:35:32.96 Carousel.home.seek 53 @15:35:33.24 Selecting Cartridge 49 @15:35:33.53 Carousel.seek 49 @15:35:34.69 Lysis1.in :fast @15:35:34.75 Lysis1.seek :locked @15:35:37.37 Plunge.seek :locked @15:35:37.53 Air.in :fast @15:35:37.59 Air.seek :locked @15:35:39.17
Carousel.reconfigure :locked @15:35:39.33 Powering cartridge bus @15:35:41.98 Gate.setIR 1750,5000,0,6 @15:35:42.10 Gate.setIR 0,5000,0,6 @15:35:42.17 PuckHeater.configure :default @15:35:42.22 Gate.power :heat,:ON,limit to 5000mA @15:35:42.90 Mounted lysis Cartridge @15:35:42.92 WARNING: Using default puck temperature sensor calibration @15:35:42.94 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:35:45.09 SPR.command "read_sequences" @15:35:45.42 SPR.command "stats_on" @15:35:45.58 SPR.command "start 0 1" @15:35:46.28 SPR.start /var/log/esp/spr23jul2415h.spr.bz2 @15:35:48.27 Waiting for baseline SPR stabilization @15:35:48.29 Handoff.select :waste @15:35:55.00 Syringe tare is 18% of full scale @15:35:55.02 PinPort.in :locking @15:35:55.08 PinPort.seek :locked @15:35:57.62 Archive.in :fast @15:35:57.68 Lysis2.in :fast @15:35:57.73 Lysis1.seek 500uL @15:35:57.89 Archive.seek :full @15:35:57.98 Lysis2.seek 500uL @15:36:04.22 delayUntil "15:45:47.80" @15:45:47.82 Lysis2.in :DAmix @15:45:47.94 Lysis1.in :DAmix @15:45:48.47 Lysis2.seek 250uL @15:45:48.54 Lysis1.seek 250uL @15:46:23.64 delay 5 seconds @15:46:28.66 Handoff.select SPR @15:46:28.80 SPR.command "start 2 14" @15:46:30.01 SPR stabilized @15:46:30.08 Additional SPR Analyses Skipped due to Insufficient Volume @15:46:30.29 Intake.close @15:46:30.37 Exhaust.close @15:46:30.47 Bypass.close @15:46:30.52 Handoff.select :close @15:46:30.78
Gate.power :heat,:OFF @15:46:30.80 Gate.power :cartridge,:OFF @15:46:30.89 PinPort.in :unlocking @15:46:31.00 PinPort.seek :clear @15:46:32.92 Carousel.in :default @15:46:33.04 Carousel.home.seek 49 @15:46:33.37 Plunge.seek :locked @15:46:33.55 Archive.in :unlocking @15:46:33.69 Archive.seek :clear @15:46:33.79 Air.in :unlocking @15:46:33.99 Air.seek :clear @15:46:34.10 Lysis1.in :unlocking @15:46:34.24 Lysis1.seek :clear @15:46:34.35 Lysis2.in :unlocking @15:46:34.43 Lysis2.seek :clear @15:46:42.01 Ended log spr23jul2415h.spr.bz2 @15:46:42.03
SPR.command "stats_off" @15:46:43.21 Gate.power :motor,:OFF @15:46:43.23 Gate.power :core,:OFF @15:46:43.24 Gate.power :analytic1,:OFF @15:46:43.25 Gate.power :analytic2,:OFF @15:46:43.27 Gate.power :main,:OFF @15:46:43.28 Safely stopped and ready to power off @15:46:43.79 Gate.power :sampler,:OFF,limit to 3500mA @15:46:47.05 Gate.power :main,:ON,limit to 5000mA @15:46:47.07 Gate.power :analytic1,:ON,limit to 5000mA @15:46:47.09 Gate.power :analytic2,:ON,limit to 5000mA @15:46:47.16 Powering core bus @15:46:49.52 SPR Module 1, FW Rev. B, FPGA Rev. C @15:46:49.94
Archive.configure :default @15:46:50.04 Air.configure :default @15:46:50.10 Lysis1.configure :default @15:46:50.16 Lysis2.configure :default @15:46:50.26 Plunge.configure :default @15:46:50.35 SamplePump.configure :default @15:46:50.44 Toroid.configure :default @15:46:50.56 Carousel.configure :default @15:46:50.65 Pressure.configure :default @15:46:50.74 ExtraPump.configure :default @15:46:50.82 PinPort.configure :default @15:46:50.88 All elf controllers running firmware version 4.84 @15:46:50.90 Gate.power :motor,:ON,limit to 5000mA @15:46:51.10 Archive.home.jog 0 @15:46:51.90 PinPort.home.jog 0 @15:46:51.94 Toroid.home.jog 0 @15:46:51.98 Plunge.home.jog 0 @15:46:52.01 Lysis1.home.jog 0 @15:46:52.05 Air.home.jog 0 @15:46:52.07 Carousel.home.jog 0 @15:46:52.09 Lysis2.home.jog 0 @15:46:53.03 Archive.in :homing @15:46:53.34 Archive.jog 1370 @15:46:53.42 PinPort.in :homing @15:46:53.45 Toroid.in :homing @15:46:53.48 Lysis2.in :homing @15:46:53.52 Air.in :homing @15:46:53.55 Plunge.in :homing @15:46:53.60 Lysis1.in :homing @15:46:53.95 PinPort.jog -113 @15:46:54.02 Toroid.jog 588 @15:46:54.06 Lysis2.jog 1397 @15:46:54.11 Air.jog 1361 @15:46:54.14 Lysis1.jog 1382 @15:46:54.23 Plunge.jog 1558 @15:46:54.63 PinPort.setRawPosition! 0 @15:46:54.71 PinPort.seek :home @15:46:54.83 Archive.setRawPosition! 50 @15:46:54.88 PinPort.in :default @15:46:54.97 Archive.seek :home @15:46:55.30 Archive.in :default @15:46:55.47 Lysis2.setRawPosition! 50 @15:46:55.56 Lysis1.setRawPosition! 50 @15:46:55.60 Air.setRawPosition! 50 @15:46:55.63 Lysis2.seek :home @15:46:55.80 Lysis1.seek :home @15:46:55.83 Air.seek :home @15:46:55.99 Plunge.setRawPosition! 50 @15:46:56.04 Plunge.seek :home @15:46:56.15 Lysis2.in :default @15:46:56.72 Lysis1.in :default @15:46:56.85 Air.in :default @15:46:56.99 Plunge.in :default @15:46:57.06 Toroid.setRawPosition! 0 @15:46:57.15 Toroid.seek :home @15:46:57.26 Toroid.in :default @15:46:57.42
Toroid.seek :clear @15:46:59.03 Carousel.in :default @15:46:59.16 Carousel.home.seek 49 @15:46:59.42 Selecting Cartridge 48 @15:46:59.73 Carousel.seek 48 @15:47:00.27 Lysis1.in :fast @15:47:00.33 Lysis1.seek :locked @15:47:02.95 Plunge.seek :locked @15:47:03.11 Air.in :fast @15:47:03.18 Air.seek :locked @15:47:04.77
Carousel.reconfigure :locked @15:47:04.94 Powering cartridge bus @15:47:07.59 Gate.setIR 1750,5000,0,6 @15:47:07.72 Gate.setIR 0,5000,0,6 @15:47:07.78 PuckHeater.configure :default @15:47:07.85 Gate.power :heat,:ON,limit to 5000mA @15:47:08.47 Mounted lysis Cartridge @15:47:08.51 WARNING: Using default puck temperature sensor calibration @15:47:08.53 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:47:10.10 SPR.command "read_sequences" @15:47:10.20 SPR.command "stats_on" @15:47:10.36 SPR.command "start 0 1" @15:47:11.06 SPR.start /var/log/esp/spr23jul2415h#2.spr.bz2 @15:47:13.05 Waiting for baseline SPR stabilization @15:47:13.07 Handoff.select :waste @15:47:20.32 Syringe tare is 18% of full scale @15:47:20.49 PinPort.in :locking @15:47:20.56 PinPort.seek :locked @15:47:23.11 Archive.in :fast @15:47:23.16 Lysis2.in :fast @15:47:23.27 Lysis1.seek 500uL @15:47:23.38 Archive.seek :full @15:47:23.50 Lysis2.seek 500uL @15:47:29.78 delayUntil "15:57:12.59" @15:57:12.60 Lysis2.in :DAmix @15:57:13.09 Lysis1.in :DAmix @15:57:13.66 Lysis2.seek 250uL @15:57:13.70 Lysis1.seek 250uL @15:57:48.84 delay 5 seconds @15:57:53.86 Handoff.select SPR @15:57:53.98 SPR.command "start 2 14" @15:57:55.24 SPR stabilized @15:57:55.33 Additional SPR Analyses Skipped due to Insufficient Volume @15:57:55.56 Handoff.select :close @15:57:55.73
Gate.power :heat,:OFF @15:57:55.75 Gate.power :cartridge,:OFF @15:57:55.85 PinPort.in :unlocking @15:57:55.92 PinPort.seek :clear @15:57:57.83 Carousel.in :default @15:57:57.96 Carousel.home.seek 48 @15:57:58.30 Plunge.seek :locked @15:57:58.49 Archive.in :unlocking @15:57:58.70 Archive.seek :clear @15:57:58.78 Air.in :unlocking @15:57:58.95 Air.seek :clear @15:57:59.06 Lysis1.in :unlocking @15:57:59.21 Lysis1.seek :clear @15:57:59.28 Lysis2.in :unlocking @15:57:59.41 Lysis2.seek :clear @15:58:07.22 Ended log spr23jul2415h#2.spr.bz2 @15:58:07.24
SPR.command "stats_off" @15:58:07.68 Gate.power :motor,:OFF @15:58:07.69 Gate.power :core,:OFF @15:58:07.71 Gate.power :analytic1,:OFF @15:58:07.72 Gate.power :analytic2,:OFF @15:58:07.74 Gate.power :main,:OFF @15:58:07.75 Safely stopped and ready to power off @15:58:07.90 Gate.power :sampler,:OFF,limit to 3500mA @15:58:11.52 Gate.power :main,:ON,limit to 5000mA @15:58:11.53 Gate.power :analytic1,:ON,limit to 5000mA @15:58:11.55 Gate.power :analytic2,:ON,limit to 5000mA @15:58:11.61 Powering core bus @15:58:14.08 SPR Module 1, FW Rev. B, FPGA Rev. C @15:58:14.28
Archive.configure :default @15:58:14.38 Air.configure :default @15:58:14.44 Lysis1.configure :default @15:58:14.53 Lysis2.configure :default @15:58:14.62 Plunge.configure :default @15:58:14.71 SamplePump.configure :default @15:58:14.81 Toroid.configure :default @15:58:14.90 Carousel.configure :default @15:58:14.99 Pressure.configure :default @15:58:15.08 ExtraPump.configure :default @15:58:15.14 PinPort.configure :default @15:58:15.20 All elf controllers running firmware version 4.84 @15:58:15.22 Gate.power :motor,:ON,limit to 5000mA @15:58:15.57 Archive.home.jog 0 @15:58:16.21 Carousel.home.jog 0 @15:58:16.26 Plunge.home.jog 0 @15:58:16.30 Lysis2.home.jog 0 @15:58:16.33 Air.home.jog 0 @15:58:16.36 PinPort.home.jog 0 @15:58:16.39 Toroid.home.jog 0 @15:58:16.42 Lysis1.home.jog 0 @15:58:17.21 Archive.in :homing @15:58:17.58 Archive.jog 1370 @15:58:17.66 PinPort.in :homing @15:58:17.68 Plunge.in :homing @15:58:17.71 Lysis1.in :homing @15:58:17.76 Lysis2.in :homing @15:58:17.80 Air.in :homing @15:58:17.84 Toroid.in :homing @15:58:18.18 PinPort.jog -112 @15:58:18.23 Lysis2.jog 1396 @15:58:18.27 Lysis1.jog 1377 @15:58:18.30 Plunge.jog 1561 @15:58:18.35 Air.jog 1363 @15:58:18.38 Toroid.jog 599 @15:58:18.87 PinPort.setRawPosition! 0 @15:58:18.92 PinPort.seek :home @15:58:19.02 PinPort.in :default @15:58:19.13 Archive.setRawPosition! 50 @15:58:19.19 Archive.seek :home @15:58:19.46 Archive.in :default @15:58:19.74 Lysis2.setRawPosition! 50 @15:58:19.77 Lysis1.setRawPosition! 50 @15:58:19.87 Lysis2.seek :home @15:58:19.91 Lysis1.seek :home @15:58:20.06 Plunge.setRawPosition! 50 @15:58:20.09 Air.setRawPosition! 50 @15:58:20.19 Plunge.seek :home @15:58:20.23 Air.seek :home @15:58:20.43 Lysis1.in :default @15:58:20.48 Lysis2.in :default @15:58:20.65 Plunge.in :default @15:58:20.70 Air.in :default @15:58:20.84 Toroid.setRawPosition! 0 @15:58:20.89 Toroid.seek :home @15:58:20.99 Toroid.in :default @15:58:21.59
Toroid.seek :clear @15:58:23.20 Carousel.in :default @15:58:23.35 Carousel.home.seek 48 @15:58:23.61 Selecting Cartridge 47 @15:58:23.91 Carousel.seek 47 @15:58:24.50 Lysis1.in :fast @15:58:24.57 Lysis1.seek :locked @15:58:27.19 Plunge.seek :locked @15:58:27.36 Air.in :fast @15:58:27.43 Air.seek :locked @15:58:29.00
Carousel.reconfigure :locked @15:58:29.16 Powering cartridge bus @15:58:31.81 Gate.setIR 1750,5000,0,6 @15:58:31.94 Gate.setIR 0,5000,0,6 @15:58:32.00 PuckHeater.configure :default @15:58:32.06 Gate.power :heat,:ON,limit to 5000mA @15:58:32.67 Mounted lysis Cartridge @15:58:32.69 WARNING: Using default puck temperature sensor calibration @15:58:32.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:58:34.27 SPR.command "read_sequences" @15:58:34.66 SPR.command "stats_on" @15:58:34.82 SPR.command "start 0 1" @15:58:35.52 SPR.start /var/log/esp/spr23jul2415h#3.spr.bz2 @15:58:37.52 Waiting for baseline SPR stabilization @15:58:37.53 Handoff.select :waste @15:58:44.23 Syringe tare is 18% of full scale @15:58:44.26 PinPort.in :locking @15:58:44.32 PinPort.seek :locked /home/esp/esp2/lib/schedule.rb:729: [BUG] Segmentation fault ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] @17:27:52.88PDT24-Jul-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @17:27:54.48 >BREAK< ESP3 Gateway v1.42 for core bus @17:27:54.53 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:27:57.90 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @17:27:58.51 Sea pressure (Abs) tare is 6% of full scale @17:28:00.49 Polling Can every 2:00 @17:28:00.57 Loading /home/esp/esp2/bin/processAll mission @17:28:00.82 Sea pressure (Abs) tare is 6% of full scale @17:28:00.84 Sampling default goal volumes @17:28:00.86 Options: {extraDilutions: [1,1]} Cartridge group: standard @17:28:01.01 Gate.power :sampler,:OFF,limit to 3500mA @17:28:01.06 Gate.power :analytic1,:ON,limit to 5000mA @17:28:01.07 Gate.power :analytic2,:ON,limit to 5000mA @17:28:01.94 SPR Module 1, FW Rev. B, FPGA Rev. C @17:28:02.18
Archive.configure :default @17:28:02.34 Air.configure :default @17:28:02.47 Lysis1.configure :default @17:28:02.63 Lysis2.configure :default @17:28:02.79 Plunge.configure :default @17:28:02.95 SamplePump.configure :default @17:28:03.11 Toroid.configure :default @17:28:03.17 Toroid.hold @17:28:03.24 Toroid.configure :default @17:28:03.40 Carousel.configure :default @17:28:03.46 Carousel.hold @17:28:03.53 Carousel.configure :default @17:28:03.69 Pressure.configure :default @17:28:03.84 ExtraPump.configure :default @17:28:04.07 PinPort.configure :default @17:28:04.19 All elf controllers running firmware version 4.84 @17:28:05.67 Toroid.seek :clear @17:28:05.84 Carousel.in :default @17:28:06.10 Carousel.home.seek 47 @17:28:06.50 Selecting Cartridge 49 @17:28:06.98 Carousel.seek 49 @17:28:07.71 Lysis1.in :fast @17:28:07.78 Lysis1.seek :locked @17:28:10.40 Plunge.seek :locked @17:28:10.60 Air.in :fast @17:28:10.66 Air.seek :locked @17:28:12.23
Carousel.reconfigure :locked @17:28:12.40 Powering cartridge bus @17:28:15.05 Gate.setIR 1750,5000,0,6 @17:28:15.18 Gate.setIR 0,5000,0,6 @17:28:15.25 PuckHeater.configure :default @17:28:15.31 Gate.power :heat,:ON,limit to 5000mA @17:28:16.00 Mounted lysis Cartridge @17:28:16.03 WARNING: Using default puck temperature sensor calibration @17:28:16.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:28:18.20 SPR.command "read_sequences" @17:28:18.55 SPR.command "stats_on" @17:28:18.73 SPR.command "start 0 1" @17:28:19.44 SPR.start /var/log/esp/spr23jul2417h.spr.bz2 @17:28:20.01 Session Begun @17:28:21.40 Waiting for baseline SPR stabilization @17:28:21.42 Handoff.select :waste @17:28:22.33 -> showlog @17:28:28.20 Syringe tare is 18% of full scale @17:28:28.23 PinPort.in :locking @17:28:28.35 PinPort.seek :locked @17:28:30.91 Archive.in :fast @17:28:30.97 Lysis2.in :fast @17:28:31.06 Lysis1.seek 850uL @17:28:31.24 Archive.seek :full @17:28:31.34 Lysis2.seek 850uL @17:28:36.48 delayUntil "17:38:20.95" @17:38:20.96 Lysis2.in :DAmix @17:38:21.12 Lysis1.in :DAmix @17:38:21.67 Lysis2.seek 600uL @17:38:21.72 Lysis1.seek 600uL @17:38:56.93 delay 5 seconds @17:39:01.95 Handoff.select SPR @17:39:02.11 SPR.command "start 2 14" @17:39:02.68 SPR stabilized @17:39:02.88 delay 10 seconds @17:39:12.90 SPR.command "start 2 0" @17:39:16.05 Waiting up to 11:00 for SPR to enter state 1 @17:41:13.72 SPR.command "hold_on" @17:41:15.09 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:41:15.75 Lysis2.seek 300uL @17:41:15.86 Lysis1.seek 300uL @17:41:57.90 delay 15 seconds @17:42:12.92 SPR.command "hold_off" @17:42:16.27 Waiting up to 38:00 for SPR to enter state 7 @17:56:36.88 SPRsummary:-27RIU,48.5RIU,130RIU,185RIU after 1792s @18:08:07.47 delay 10 seconds @18:08:17.49 SPR.command "start 2 0" @18:08:20.68 Waiting up to 11:00 for SPR to enter state 1 @18:10:18.78 SPR.command "hold_on" @18:10:20.17 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:10:21.23 Lysis2.seek 150uL @18:10:21.27 Lysis1.seek 150uL @18:10:42.41 delay 15 seconds @18:10:57.45 SPR.command "hold_off" @18:11:00.01 Waiting up to 38:00 for SPR to enter state 7 @18:36:50.69 Additional SPR Analyses Skipped due to Insufficient Volume @18:36:50.96 Intake.close @18:36:51.00 Exhaust.close @18:36:51.10 Bypass.close @18:36:51.24 Handoff.select :close @18:36:51.49
Gate.power :heat,:OFF @18:36:51.54 Gate.power :cartridge,:OFF @18:36:51.68 PinPort.in :unlocking @18:36:51.77 PinPort.seek :clear @18:36:53.72 Carousel.in :default @18:36:53.89 Carousel.home.seek 49 @18:36:54.25 Plunge.seek :locked @18:36:54.52 Archive.in :unlocking @18:36:54.71 Archive.seek :clear @18:36:54.86 Air.in :unlocking @18:36:55.51 Air.seek :clear @18:36:55.66 Lysis1.in :unlocking @18:36:55.84 Lysis1.seek :clear @18:36:56.00 Lysis2.in :unlocking @18:36:56.11 Lysis2.seek :clear @18:37:14.15 Ended log spr23jul2417h.spr.bz2 @18:37:14.17
SPR.command "stats_off" @18:37:14.85 Gate.power :motor,:OFF @18:37:14.86 Gate.power :core,:OFF @18:37:14.90 Gate.power :analytic1,:OFF @18:37:14.94 Gate.power :analytic2,:OFF @18:37:14.97 Gate.power :main,:OFF @18:37:15.00 Safely stopped and ready to power off @18:37:15.59 Gate.power :sampler,:OFF,limit to 3500mA @18:37:18.71 Gate.power :main,:ON,limit to 5000mA @18:37:18.73 Gate.power :analytic1,:ON,limit to 5000mA @18:37:18.78 Gate.power :analytic2,:ON,limit to 5000mA @18:37:18.89 Powering core bus @18:37:21.23 SPR Module 1, FW Rev. B, FPGA Rev. C @18:37:21.67
Archive.configure :default @18:37:21.79 Air.configure :default @18:37:21.88 Lysis1.configure :default @18:37:21.98 Lysis2.configure :default @18:37:22.10 Plunge.configure :default @18:37:22.22 SamplePump.configure :default @18:37:22.34 Toroid.configure :default @18:37:22.47 Carousel.configure :default @18:37:22.62 Pressure.configure :default @18:37:22.74 ExtraPump.configure :default @18:37:22.83 PinPort.configure :default @18:37:22.93 All elf controllers running firmware version 4.84 @18:37:22.96 Gate.power :motor,:ON,limit to 5000mA @18:37:23.27 Archive.home.jog 0 @18:37:24.04 Carousel.home.jog 0 @18:37:24.10 Toroid.home.jog 0 @18:37:24.15 Lysis2.home.jog 0 @18:37:24.20 Lysis1.home.jog 0 @18:37:24.25 PinPort.home.jog 0 @18:37:24.30 Plunge.home.jog 0 @18:37:24.34 Air.home.jog 0 @18:37:25.14 Archive.in :homing @18:37:25.51 Archive.jog 1367 @18:37:25.79 PinPort.in :homing @18:37:25.82 Toroid.in :homing @18:37:25.88 Lysis2.in :homing @18:37:25.94 Air.in :homing @18:37:26.01 Plunge.in :homing @18:37:26.05 Lysis1.in :homing @18:37:26.44 PinPort.jog -114 @18:37:26.48 Toroid.jog 599 @18:37:26.55 Lysis2.jog 1393 @18:37:26.63 Plunge.jog 1554 @18:37:26.69 Air.jog 1363 @18:37:26.77 Lysis1.jog 1382 @18:37:27.64 Archive.setRawPosition! 50 @18:37:27.76 PinPort.setRawPosition! 0 @18:37:27.89 Archive.seek :home @18:37:27.94 PinPort.seek :home @18:37:28.20 Lysis2.setRawPosition! 50 @18:37:28.23 PinPort.in :default @18:37:28.40 Lysis2.seek :home @18:37:28.61 Plunge.setRawPosition! 50 @18:37:28.63 Lysis1.setRawPosition! 50 @18:37:28.68 Archive.in :default @18:37:28.73 Air.setRawPosition! 50 @18:37:28.93 Plunge.seek :home @18:37:28.96 Lysis1.seek :home @18:37:29.10 Air.seek :home @18:37:29.31 Toroid.setRawPosition! 0 @18:37:29.37 Lysis2.in :default @18:37:29.48 Toroid.seek :home @18:37:29.74 Plunge.in :default @18:37:29.76 Lysis1.in :default @18:37:29.81 Toroid.in :default @18:37:29.86 Air.in :default @18:37:30.41
Toroid.seek :clear @18:37:32.06 Carousel.in :default @18:37:32.23 Carousel.home.seek 49 @18:37:32.54 Selecting Cartridge 48 @18:37:32.91 Carousel.seek 48 @18:37:33.47 Lysis1.in :fast @18:37:33.56 Lysis1.seek :locked @18:37:36.22 Plunge.seek :locked @18:37:36.41 Air.in :fast @18:37:36.52 Air.seek :locked @18:37:38.14
Carousel.reconfigure :locked @18:37:38.33 Powering cartridge bus @18:37:40.99 Gate.setIR 1750,5000,0,6 @18:37:41.13 Gate.setIR 0,5000,0,6 @18:37:41.23 PuckHeater.configure :default @18:37:41.32 Gate.power :heat,:ON,limit to 5000mA @18:37:41.93 Mounted lysis Cartridge @18:37:41.97 WARNING: Using default puck temperature sensor calibration @18:37:42.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:37:43.67 SPR.command "read_sequences" @18:37:43.78 SPR.command "stats_on" @18:37:43.95 SPR.command "start 0 1" @18:37:44.66 SPR.start /var/log/esp/spr23jul2418h.spr.bz2 @18:37:46.64 Waiting for baseline SPR stabilization @18:37:46.66 Handoff.select :waste @18:37:53.41 Syringe tare is 18% of full scale @18:37:53.44 PinPort.in :locking @18:37:53.55 PinPort.seek :locked @18:37:56.12 Archive.in :fast @18:37:56.17 Lysis2.in :fast @18:37:56.29 Lysis1.seek 850uL @18:37:56.47 Archive.seek :full @18:37:56.60 Lysis2.seek 850uL @18:38:01.75 delayUntil "18:47:46.20" @18:43:11.25 Session Begun @18:43:15.73 -> cd /var/log/esp @18:43:15.77 SyntaxError in bill#2 -- compile error bill#2:1: unknown regexp options - lg @18:43:29.16 -> exit @18:43:29.18 Session Ended @18:47:46.21 Lysis2.in :DAmix @18:47:46.38 Lysis1.in :DAmix @18:47:46.93 Lysis2.seek 600uL @18:47:46.98 Lysis1.seek 600uL @18:48:22.16 delay 5 seconds @18:48:27.18 Handoff.select SPR @18:48:27.33 SPR.command "start 2 14" @18:48:27.91 SPR stabilized @18:48:28.10 delay 10 seconds @18:48:38.12 SPR.command "start 2 0" @18:48:41.28 Waiting up to 11:00 for SPR to enter state 1 @18:50:38.95 SPR.command "hold_on" @18:50:40.85 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:50:41.47 Lysis2.seek 300uL @18:50:41.56 Lysis1.seek 300uL @18:51:23.63 delay 15 seconds @18:51:38.66 SPR.command "hold_off" @18:51:41.93 Waiting up to 38:00 for SPR to enter state 7 @19:06:01.16 SPRsummary:14.6RIU,43RIU,81.4RIU,119RIU after 1791s @19:17:31.74 delay 10 seconds @19:17:41.76 SPR.command "start 2 0" @19:17:44.44 Waiting up to 11:00 for SPR to enter state 1 @19:19:42.11 SPR.command "hold_on" @19:19:44.01 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:19:44.60 Lysis2.seek 150uL @19:19:44.64 Lysis1.seek 150uL @19:20:05.78 delay 15 seconds @19:20:20.80 SPR.command "hold_off" @19:20:24.80 Waiting up to 38:00 for SPR to enter state 7 @19:46:14.95 Additional SPR Analyses Skipped due to Insufficient Volume @19:46:15.20 Handoff.select :close @19:46:15.38
Gate.power :heat,:OFF @19:46:15.40 Gate.power :cartridge,:OFF @19:46:15.52 PinPort.in :unlocking @19:46:15.61 PinPort.seek :clear @19:46:17.56 Carousel.in :default @19:46:17.76 Carousel.home.seek 48 @19:46:18.14 Plunge.seek :locked @19:46:18.35 Archive.in :unlocking @19:46:18.57 Archive.seek :clear @19:46:18.65 Air.in :unlocking @19:46:18.90 Air.seek :clear @19:46:19.04 Lysis1.in :unlocking @19:46:19.66 Lysis1.seek :clear @19:46:19.86 Lysis2.in :unlocking @19:46:19.98 Lysis2.seek :clear @19:46:36.73 Ended log spr23jul2418h.spr.bz2 @19:46:36.75
SPR.command "stats_off" @19:46:37.20 Gate.power :motor,:OFF @19:46:37.23 Gate.power :core,:OFF @19:46:37.27 Gate.power :analytic1,:OFF @19:46:37.31 Gate.power :analytic2,:OFF @19:46:37.35 Gate.power :main,:OFF @19:46:37.38 Safely stopped and ready to power off @19:46:37.91 Gate.power :sampler,:OFF,limit to 3500mA @19:46:41.07 Gate.power :main,:ON,limit to 5000mA @19:46:41.09 Gate.power :analytic1,:ON,limit to 5000mA @19:46:41.14 Gate.power :analytic2,:ON,limit to 5000mA @19:46:41.24 Powering core bus @19:46:43.58 SPR Module 1, FW Rev. B, FPGA Rev. C @19:46:44.02
Archive.configure :default @19:46:44.15 Air.configure :default @19:46:44.24 Lysis1.configure :default @19:46:44.33 Lysis2.configure :default @19:46:44.45 Plunge.configure :default @19:46:44.61 SamplePump.configure :default @19:46:44.73 Toroid.configure :default @19:46:44.86 Carousel.configure :default @19:46:44.98 Pressure.configure :default @19:46:45.10 ExtraPump.configure :default @19:46:45.19 PinPort.configure :default @19:46:45.28 All elf controllers running firmware version 4.84 @19:46:45.31 Gate.power :motor,:ON,limit to 5000mA @19:46:45.51 Archive.home.jog 0 @19:46:46.28 Toroid.home.jog 0 @19:46:46.42 Carousel.home.jog 0 @19:46:46.47 Plunge.home.jog 0 @19:46:46.54 Lysis2.home.jog 0 @19:46:46.58 Air.home.jog 0 @19:46:46.64 Lysis1.home.jog 0 @19:46:46.69 PinPort.home.jog 0 @19:46:47.64 Archive.in :homing @19:46:48.04 Lysis2.in :homing @19:46:48.13 Archive.jog 1369 @19:46:48.19 PinPort.in :homing @19:46:48.24 Plunge.in :homing @19:46:48.30 Air.in :homing @19:46:48.36 Toroid.in :homing @19:46:48.42 Lysis1.in :homing @19:46:48.84 Lysis2.jog 1393 @19:46:48.92 PinPort.jog -112 @19:46:48.98 Air.jog 1365 @19:46:49.04 Plunge.jog 1559 @19:46:49.12 Toroid.jog 600 @19:46:49.18 Lysis1.jog 1378 @19:46:49.57 PinPort.setRawPosition! 0 @19:46:49.65 PinPort.seek :home @19:46:49.80 PinPort.in :default @19:46:49.82 Archive.setRawPosition! 50 @19:46:49.98 Archive.seek :home @19:46:50.70 Lysis2.setRawPosition! 50 @19:46:50.74 Lysis1.setRawPosition! 50 @19:46:50.79 Plunge.setRawPosition! 50 @19:46:50.84 Air.setRawPosition! 50 @19:46:51.06 Lysis2.seek :home @19:46:51.12 Plunge.seek :home @19:46:51.16 Lysis1.seek :home @19:46:51.20 Air.seek :home @19:46:51.58 Toroid.setRawPosition! 0 @19:46:51.61 Archive.in :default @19:46:51.77 Toroid.seek :home @19:46:52.00 Plunge.in :default @19:46:52.05 Lysis2.in :default @19:46:52.09 Air.in :default @19:46:52.15 Toroid.in :default @19:46:52.22 Lysis1.in :default @19:46:52.83
Toroid.seek :clear @19:46:54.47 Carousel.in :default @19:46:54.66 Carousel.home.seek 48 @19:46:54.94 Selecting Cartridge 47 @19:46:55.28 Carousel.seek 47 @19:46:55.83 Lysis1.in :fast @19:46:55.92 Lysis1.seek :locked @19:46:58.61 Plunge.seek :locked @19:46:58.81 Air.in :fast @19:46:58.90 Air.seek :locked @19:47:00.51
Carousel.reconfigure :locked @19:47:00.74 Powering cartridge bus @19:47:03.39 Gate.setIR 1750,5000,0,6 @19:47:03.52 Gate.setIR 0,5000,0,6 @19:47:03.62 PuckHeater.configure :default @19:47:03.71 Gate.power :heat,:ON,limit to 5000mA @19:47:04.33 Mounted lysis Cartridge @19:47:04.36 WARNING: Using default puck temperature sensor calibration @19:47:04.40 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:47:06.05 SPR.command "read_sequences" @19:47:06.15 SPR.command "stats_on" @19:47:06.32 SPR.command "start 0 1" @19:47:07.02 SPR.start /var/log/esp/spr23jul2419h.spr.bz2 @19:47:09.00 Waiting for baseline SPR stabilization @19:47:09.02 Handoff.select :waste @19:47:15.94 Syringe tare is 18% of full scale @19:47:15.97 PinPort.in :locking @19:47:16.09 PinPort.seek :locked @19:47:18.68 Archive.in :fast @19:47:18.80 Lysis2.in :fast @19:47:18.89 Lysis1.seek 850uL @19:47:18.93 Archive.seek :full @19:47:19.14 Lysis2.seek 850uL @19:47:24.27 delayUntil "19:57:08.59" @19:57:08.60 Lysis2.in :DAmix @19:57:08.77 Lysis1.in :DAmix @19:57:09.28 Lysis2.seek 600uL @19:57:09.34 Lysis1.seek 600uL @19:57:44.51 delay 5 seconds @19:57:49.55 Handoff.select SPR @19:57:49.70 SPR.command "start 2 14" @19:57:50.80 SPR stabilized @19:57:50.98 delay 10 seconds @19:58:01.01 SPR.command "start 2 0" @19:58:03.63 Waiting up to 11:00 for SPR to enter state 1 @20:00:01.31 SPR.command "hold_on" @20:00:02.70 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:00:03.36 Lysis2.seek 300uL @20:00:03.40 Lysis1.seek 300uL @20:00:45.50 delay 15 seconds @20:01:00.54 SPR.command "hold_off" @20:01:03.35 Waiting up to 38:00 for SPR to enter state 7 @20:15:23.53 SPRsummary:23.5RIU,27.7RIU,60RIU,65RIU after 1791s @20:26:54.11 delay 10 seconds @20:27:04.13 SPR.command "start 2 0" @20:27:06.80 Waiting up to 11:00 for SPR to enter state 1 @20:29:04.49 SPR.command "hold_on" @20:29:06.32 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:29:06.94 Lysis2.seek 150uL @20:29:07.03 Lysis1.seek 150uL @20:29:28.15 delay 15 seconds @20:29:43.17 SPR.command "hold_off" @20:29:46.23 Waiting up to 38:00 for SPR to enter state 7 @20:55:37.35 Additional SPR Analyses Skipped due to Insufficient Volume @20:55:37.60 Handoff.select :close @20:55:37.78
Gate.power :heat,:OFF @20:55:37.80 Gate.power :cartridge,:OFF @20:55:37.93 PinPort.in :unlocking @20:55:38.02 PinPort.seek :clear @20:55:39.97 Carousel.in :default @20:55:40.18 Carousel.home.seek 47 @20:55:40.58 Plunge.seek :locked @20:55:40.79 Archive.in :unlocking @20:55:40.98 Archive.seek :clear @20:55:41.07 Air.in :unlocking @20:55:41.29 Air.seek :clear @20:55:41.97 Lysis1.in :unlocking @20:55:42.11 Lysis1.seek :clear @20:55:42.30 Lysis2.in :unlocking @20:55:42.41 Lysis2.seek :clear @20:55:59.19 Ended log spr23jul2419h.spr.bz2 @20:55:59.21
SPR.command "stats_off" @20:55:59.60 Gate.power :motor,:OFF @20:55:59.62 Gate.power :core,:OFF @20:55:59.66 Gate.power :analytic1,:OFF @20:55:59.70 Gate.power :analytic2,:OFF @20:55:59.73 Gate.power :main,:OFF @20:55:59.76 Safely stopped and ready to power off @20:56:00.27 Cartridge::None in MAIN -- Not enough standard cartridges remain @20:56:00.36 Session Ended @10:32:57.37PDT25-Jul-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @10:32:58.93 >BREAK< ESP3 Gateway v1.42 for core bus @10:32:58.95 >BREAK< ESP3 Gateway v1.42 for cartridge bus @10:33:02.14 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @10:33:02.77 Sea pressure (Abs) tare is 6% of full scale @10:33:04.71 Polling Can every 2:00 @10:33:04.75 Loading /home/esp/esp2/bin/processAll mission @10:33:04.99 Sea pressure (Abs) tare is 6% of full scale @10:33:05.00 Sampling default goal volumes @10:33:05.02 Options: {extraDilutions: [1,1]} Cartridge group: standard @10:33:05.18 Gate.power :sampler,:OFF,limit to 3500mA @10:33:05.19 Gate.power :analytic1,:ON,limit to 5000mA @10:33:05.20 Gate.power :analytic2,:ON,limit to 5000mA @10:33:06.27 Archive.configure :default @10:33:06.42 Air.configure :default @10:33:06.58 Lysis1.configure :default @10:33:06.71 Lysis2.configure :default @10:33:06.83 SPR Module 1, FW Rev. B, FPGA Rev. C @10:33:06.93
Plunge.configure :default @10:33:07.08 SamplePump.configure :default @10:33:07.24 Toroid.configure :default @10:33:07.30 Toroid.hold @10:33:07.37 Toroid.configure :default @10:33:07.52 Carousel.configure :default @10:33:07.58 Carousel.hold @10:33:07.65 Carousel.configure :default @10:33:07.82 Pressure.configure :default @10:33:07.97 ExtraPump.configure :default @10:33:08.10 PinPort.configure :default @10:33:08.16 All elf controllers running firmware version 4.84 @10:33:09.01 Toroid.seek :clear @10:33:09.09 Carousel.in :default @10:33:09.22 Carousel.home.seek 47 @10:33:09.49 Selecting Cartridge 46 @10:33:09.79 Carousel.seek 46 @10:33:10.32 Lysis1.in :fast @10:33:10.38 Lysis1.seek :locked @10:33:13.02 Plunge.seek :locked @10:33:13.18 Air.in :fast @10:33:13.24 Air.seek :locked @10:33:14.81
Carousel.reconfigure :locked @10:33:14.97 Powering cartridge bus @10:33:17.62 Gate.setIR 1750,5000,0,6 @10:33:17.74 Gate.setIR 0,5000,0,6 @10:33:17.81 PuckHeater.configure :default @10:33:17.87 Gate.power :heat,:ON,limit to 5000mA @10:33:18.57 Mounted lysis Cartridge @10:33:18.59 WARNING: Using default puck temperature sensor calibration @10:33:18.61 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:33:20.68 SPR.command "read_sequences" @10:33:20.92 SPR.command "stats_on" @10:33:21.09 SPR.command "start 0 1" @10:33:21.79 SPR.start /var/log/esp/spr23jul2510h.spr.bz2 @10:33:24.27 Waiting for baseline SPR stabilization @10:33:24.28 Handoff.select :waste @10:33:30.98 Syringe tare is 18% of full scale @10:33:31.00 PinPort.in :locking @10:33:31.06 PinPort.seek :locked @10:33:33.60 Archive.in :fast @10:33:33.67 Lysis2.in :fast @10:33:33.79 Lysis1.seek 850uL @10:33:33.93 Archive.seek :full @10:33:34.01 Lysis2.seek 850uL @10:33:39.10 delayUntil "10:43:24.23" @10:43:24.25 Lysis2.in :DAmix @10:43:24.38 Lysis1.in :DAmix @10:43:24.94 Lysis2.seek 600uL @10:43:25.02 Lysis1.seek 600uL @10:44:00.07 delay 5 seconds @10:44:05.10 Handoff.select SPR @10:44:05.23 SPR.command "start 2 14" @10:44:06.48 SPR stabilized @10:44:06.69 delay 10 seconds @10:44:16.71 SPR.command "start 2 0" @10:44:19.36 Waiting up to 11:00 for SPR to enter state 1 @10:46:16.99 SPR.command "hold_on" @10:46:18.91 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @10:46:19.50 Lysis2.seek 300uL @10:46:19.54 Lysis1.seek 300uL @10:47:01.60 delay 15 seconds @10:47:16.62 SPR.command "hold_off" @10:47:20.52 Waiting up to 38:00 for SPR to enter state 7 @11:01:40.16 SPRsummary:35.6RIU,63.5RIU,138RIU,134RIU after 1793s @11:13:10.74 delay 10 seconds @11:13:20.76 SPR.command "start 2 0" @11:13:23.47 Waiting up to 11:00 for SPR to enter state 1 @11:15:21.11 SPR.command "hold_on" @11:15:22.56 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @11:15:23.12 Lysis2.seek 150uL @11:15:23.18 Lysis1.seek 150uL @11:15:44.30 delay 15 seconds @11:15:59.32 SPR.command "hold_off" @11:16:02.37 Waiting up to 38:00 for SPR to enter state 7 @11:22:33.49 Session Begun @11:22:37.23 -> showlog @11:22:47.25 -> cd /var/log/esp @11:22:47.29 SyntaxError in bill -- compile error bill:2: unknown regexp options - lg @11:22:54.79 -> exit @11:22:54.81 Session Ended @11:41:53.05 Additional SPR Analyses Skipped due to Insufficient Volume @11:41:53.25 Intake.close @11:41:53.30 Exhaust.close @11:41:53.42 Bypass.close @11:41:53.50 Handoff.select :close @11:41:53.71
Gate.power :heat,:OFF @11:41:53.73 Gate.power :cartridge,:OFF @11:41:53.81 PinPort.in :unlocking @11:41:53.89 PinPort.seek :clear @11:41:55.84 Carousel.in :default @11:41:55.97 Carousel.home.seek 46 @11:41:56.79 Plunge.seek :locked @11:41:56.98 Archive.in :unlocking @11:41:57.14 Archive.seek :clear @11:41:57.24 Air.in :unlocking @11:41:57.40 Air.seek :clear @11:41:57.50 Lysis1.in :unlocking @11:41:57.69 Lysis1.seek :clear @11:41:57.75 Lysis2.in :unlocking @11:41:57.91 Lysis2.seek :clear @11:42:14.51 Ended log spr23jul2510h.spr.bz2 @11:42:14.55
SPR.command "stats_off" @11:42:15.30 Gate.power :motor,:OFF @11:42:15.32 Gate.power :core,:OFF @11:42:15.33 Gate.power :analytic1,:OFF @11:42:15.34 Gate.power :analytic2,:OFF @11:42:15.36 Gate.power :main,:OFF @11:42:15.37 Safely stopped and ready to power off @11:42:15.94 Gate.power :sampler,:OFF,limit to 3500mA @11:42:19.14 Gate.power :main,:ON,limit to 5000mA @11:42:19.16 Gate.power :analytic1,:ON,limit to 5000mA @11:42:19.17 Gate.power :analytic2,:ON,limit to 5000mA @11:42:19.24 Powering core bus @11:42:21.60 SPR Module 1, FW Rev. B, FPGA Rev. C @11:42:22.02
Archive.configure :default @11:42:22.11 Air.configure :default @11:42:22.18 Lysis1.configure :default @11:42:22.24 Lysis2.configure :default @11:42:22.34 Plunge.configure :default @11:42:22.43 SamplePump.configure :default @11:42:22.56 Toroid.configure :default @11:42:22.65 Carousel.configure :default @11:42:22.74 Pressure.configure :default @11:42:22.83 ExtraPump.configure :default @11:42:22.90 PinPort.configure :default @11:42:22.96 All elf controllers running firmware version 4.84 @11:42:22.98 Gate.power :motor,:ON,limit to 5000mA @11:42:23.36 Archive.home.jog 0 @11:42:24.06 Carousel.home.jog 0 @11:42:24.12 Plunge.home.jog 0 @11:42:24.15 Lysis1.home.jog 0 @11:42:24.18 Toroid.home.jog 0 @11:42:24.21 Lysis2.home.jog 0 @11:42:24.31 Air.home.jog 0 @11:42:24.35 PinPort.home.jog 0 @11:42:25.24 Archive.in :homing @11:42:25.56 Archive.jog 1371 @11:42:25.64 Toroid.in :homing @11:42:25.68 Lysis2.in :homing @11:42:25.72 Air.in :homing @11:42:25.75 PinPort.in :homing @11:42:25.79 Lysis1.in :homing @11:42:25.87 Plunge.in :homing @11:42:26.76 Toroid.jog 597 @11:42:26.83 Lysis2.jog 1393 @11:42:26.84 PinPort.jog -111 @11:42:26.87 Lysis1.jog 1382 @11:42:26.90 Air.jog 1365 @11:42:26.96 Plunge.jog 1557 @11:42:27.37 PinPort.setRawPosition! 0 @11:42:27.43 PinPort.seek :home @11:42:27.57 Archive.setRawPosition! 50 @11:42:27.59 PinPort.in :default @11:42:27.69 Archive.seek :home @11:42:27.99 Archive.in :default @11:42:28.24 Lysis2.setRawPosition! 50 @11:42:28.33 Lysis1.setRawPosition! 50 @11:42:28.35 Lysis2.seek :home @11:42:28.47 Lysis1.seek :home @11:42:28.62 Plunge.setRawPosition! 50 @11:42:28.66 Air.setRawPosition! 50 @11:42:28.78 Plunge.seek :home @11:42:28.81 Air.seek :home @11:42:29.00 Lysis2.in :default @11:42:29.02 Lysis1.in :default @11:42:29.20 Plunge.in :default @11:42:29.25 Toroid.setRawPosition! 0 @11:42:29.28 Air.in :default @11:42:29.40 Toroid.seek :home @11:42:29.62 Toroid.in :default @11:42:29.86
Toroid.seek :clear @11:42:31.47 Carousel.in :default @11:42:31.62 Carousel.home.seek 46 @11:42:31.92 Selecting Cartridge 45 @11:42:32.23 Carousel.seek 45 @11:42:32.77 Lysis1.in :fast @11:42:32.83 Lysis1.seek :locked @11:42:35.45 Plunge.seek :locked @11:42:35.62 Air.in :fast @11:42:35.69 Air.seek :locked @11:42:37.28
Carousel.reconfigure :locked @11:42:37.45 Powering cartridge bus @11:42:40.11 Gate.setIR 1750,5000,0,6 @11:42:40.23 Gate.setIR 0,5000,0,6 @11:42:40.30 PuckHeater.configure :default @11:42:40.36 Gate.power :heat,:ON,limit to 5000mA @11:42:40.97 Mounted lysis Cartridge @11:42:41.00 WARNING: Using default puck temperature sensor calibration @11:42:41.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:42:42.61 SPR.command "read_sequences" @11:42:43.23 SPR.command "stats_on" @11:42:44.29 SPR.command "start 0 1" @11:42:44.99 SPR.start /var/log/esp/spr23jul2511h.spr.bz2 @11:42:47.03 Waiting for baseline SPR stabilization @11:42:47.05 Handoff.select :waste @11:42:53.75 Syringe tare is 18% of full scale @11:42:53.77 PinPort.in :locking @11:42:53.83 PinPort.seek :locked @11:42:56.38 Archive.in :fast @11:42:56.44 Lysis2.in :fast @11:42:56.49 Lysis1.seek 850uL @11:42:56.71 Lysis2.seek 850uL @11:42:56.75 Archive.seek :full @11:43:01.83 delayUntil "11:52:46.59" @11:52:46.61 Lysis2.in :DAmix @11:52:46.73 Lysis1.in :DAmix @11:52:47.26 Lysis2.seek 600uL @11:52:47.32 Lysis1.seek 600uL @11:53:22.43 delay 5 seconds @11:53:27.45 Handoff.select SPR @11:53:27.59 SPR.command "start 2 14" @11:53:28.30 SPR stabilized @11:53:28.48 delay 10 seconds @11:53:38.51 SPR.command "start 2 0" @11:53:42.18 Waiting up to 11:00 for SPR to enter state 1 @11:55:40.28 SPR.command "hold_on" @11:55:41.66 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:55:42.28 Lysis2.seek 300uL @11:55:42.36 Lysis1.seek 300uL @11:56:24.35 delay 15 seconds @11:56:39.37 SPR.command "hold_off" @11:56:42.32 Waiting up to 38:00 for SPR to enter state 7 @12:11:02.50 SPRsummary:25RIU,30.2RIU,53.4RIU,61.2RIU after 1792s @12:22:33.10 delay 10 seconds @12:22:43.13 SPR.command "start 2 0" @12:22:45.79 Waiting up to 11:00 for SPR to enter state 1 @12:24:43.44 SPR.command "hold_on" @12:24:44.84 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:24:45.43 Lysis2.seek 150uL @12:24:45.47 Lysis1.seek 150uL @12:25:06.58 delay 15 seconds @12:25:21.60 SPR.command "hold_off" @12:25:24.68 Waiting up to 38:00 for SPR to enter state 7 @12:51:15.36 Additional SPR Analyses Skipped due to Insufficient Volume @12:51:15.61 Handoff.select :close @12:51:15.76
Gate.power :heat,:OFF @12:51:15.77 Gate.power :cartridge,:OFF @12:51:15.85 PinPort.in :unlocking @12:51:15.92 PinPort.seek :clear @12:51:17.83 Carousel.in :default @12:51:18.42 Carousel.home.seek 45 @12:51:18.80 Plunge.seek :locked @12:51:18.98 Archive.in :unlocking @12:51:19.19 Archive.seek :clear @12:51:19.24 Air.in :unlocking @12:51:19.45 Air.seek :clear @12:51:19.56 Lysis1.in :unlocking @12:51:19.71 Lysis1.seek :clear @12:51:19.77 Lysis2.in :unlocking @12:51:19.89 Lysis2.seek :clear @12:51:36.74 Ended log spr23jul2511h.spr.bz2 @12:51:36.75
SPR.command "stats_off" @12:51:37.63 Gate.power :motor,:OFF @12:51:37.64 Gate.power :core,:OFF @12:51:37.65 Gate.power :analytic1,:OFF @12:51:37.67 Gate.power :analytic2,:OFF @12:51:37.68 Gate.power :main,:OFF @12:51:37.69 Safely stopped and ready to power off @12:51:37.88 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:47:35.30PDT25-Jul-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @13:47:36.83 >BREAK< ESP3 Gateway v1.42 for core bus @13:47:36.86 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:47:40.04 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @13:47:40.64 Sea pressure (Abs) tare is 6% of full scale @13:47:42.59 Polling Can every 2:00 @13:47:42.63 Loading /home/esp/esp2/bin/processAll mission @13:47:42.86 Sea pressure (Abs) tare is 6% of full scale @13:47:42.87 Sampling default goal volumes @13:47:42.89 Options: {extraDilutions: [1,1]} Cartridge group: standard @13:47:43.06 Gate.power :sampler,:OFF,limit to 3500mA @13:47:43.07 Gate.power :analytic1,:ON,limit to 5000mA @13:47:43.08 Gate.power :analytic2,:ON,limit to 5000mA @13:47:44.15 Archive.configure :default @13:47:44.31 Air.configure :default @13:47:44.44 Lysis1.configure :default @13:47:44.61 SPR Module 1, FW Rev. B, FPGA Rev. C @13:47:44.68
Lysis2.configure :default @13:47:44.83 Plunge.configure :default @13:47:44.99 SamplePump.configure :default @13:47:45.15 Toroid.configure :default @13:47:45.21 Toroid.hold @13:47:45.28 Toroid.configure :default @13:47:45.43 Carousel.configure :default @13:47:45.49 Carousel.hold @13:47:45.56 Carousel.configure :default @13:47:45.73 Pressure.configure :default @13:47:45.89 ExtraPump.configure :default @13:47:46.02 PinPort.configure :default @13:47:46.08 All elf controllers running firmware version 4.84 @13:47:46.93 Toroid.seek :clear @13:47:47.02 Carousel.in :default @13:47:47.14 Carousel.home.seek 45 @13:47:47.40 Selecting Cartridge 44 @13:47:47.69 Carousel.seek 44 @13:47:48.22 Lysis1.in :fast @13:47:48.28 Lysis1.seek :locked @13:47:50.92 Plunge.seek :locked @13:47:51.08 Air.in :fast @13:47:51.14 Air.seek :locked @13:47:52.71
Carousel.reconfigure :locked @13:47:52.88 Powering cartridge bus @13:47:55.53 Gate.setIR 1750,5000,0,6 @13:47:55.65 Gate.setIR 0,5000,0,6 @13:47:55.72 PuckHeater.configure :default @13:47:55.78 Gate.power :heat,:ON,limit to 5000mA @13:47:56.45 Mounted lysis Cartridge @13:47:56.48 WARNING: Using default puck temperature sensor calibration @13:47:56.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:47:58.57 SPR.command "read_sequences" @13:47:58.66 SPR.command "stats_on" @13:47:58.83 SPR.command "start 0 1" @13:47:59.53 SPR.start /var/log/esp/spr23jul2513h.spr.bz2 @13:48:01.52 Waiting for baseline SPR stabilization @13:48:01.53 Handoff.select :waste @13:48:08.24 Syringe tare is 18% of full scale @13:48:08.26 PinPort.in :locking @13:48:08.32 PinPort.seek :locked @13:48:10.86 Archive.in :fast @13:48:10.92 Lysis2.in :fast @13:48:10.99 Lysis1.seek 850uL @13:48:11.13 Archive.seek :full @13:48:11.20 Lysis2.seek 850uL @13:48:16.28 delayUntil "13:58:01.05" @13:58:01.07 Lysis2.in :DAmix @13:58:01.19 Lysis1.in :DAmix @13:58:01.72 Lysis2.seek 600uL @13:58:01.78 Lysis1.seek 600uL @13:58:36.88 delay 5 seconds @13:58:41.90 Handoff.select SPR @13:58:42.03 SPR.command "start 2 14" @13:58:42.79 SPR stabilized @13:58:42.94 delay 10 seconds @13:58:52.97 SPR.command "start 2 0" @13:58:56.15 Waiting up to 11:00 for SPR to enter state 1 @14:00:53.80 SPR.command "hold_on" @14:00:55.21 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:00:55.81 Lysis2.seek 300uL @14:00:55.86 Lysis1.seek 300uL @14:01:37.89 delay 15 seconds @14:01:52.91 SPR.command "hold_off" @14:01:55.86 Waiting up to 38:00 for SPR to enter state 7 @14:16:16.04 SPRsummary:21.3RIU,30.6RIU,64.5RIU,75.5RIU after 1791s @14:27:46.65 delay 10 seconds @14:27:56.67 SPR.command "start 2 0" @14:27:59.33 Waiting up to 11:00 for SPR to enter state 1 @14:29:57.00 SPR.command "hold_on" @14:29:58.38 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @14:29:58.98 Lysis2.seek 150uL @14:29:59.04 Lysis1.seek 150uL @14:30:20.12 delay 15 seconds @14:30:35.14 SPR.command "hold_off" @14:30:38.23 Waiting up to 38:00 for SPR to enter state 7 @14:56:28.90 Additional SPR Analyses Skipped due to Insufficient Volume @14:56:29.11 Intake.close @14:56:29.16 Exhaust.close @14:56:29.27 Bypass.close @14:56:29.35 Handoff.select :close @14:56:29.57
Gate.power :heat,:OFF @14:56:29.59 Gate.power :cartridge,:OFF @14:56:29.67 PinPort.in :unlocking @14:56:29.74 PinPort.seek :clear @14:56:31.70 Carousel.in :default @14:56:31.83 Carousel.home.seek 44 @14:56:32.50 Plunge.seek :locked @14:56:32.75 Archive.in :unlocking @14:56:32.93 Archive.seek :clear @14:56:33.05 Air.in :unlocking @14:56:33.18 Air.seek :clear @14:56:33.24 Lysis1.in :unlocking @14:56:33.41 Lysis1.seek :clear @14:56:33.58 Lysis2.in :unlocking @14:56:33.66 Lysis2.seek :clear @14:56:50.38 Ended log spr23jul2513h.spr.bz2 @14:56:50.40
SPR.command "stats_off" @14:56:51.17 Gate.power :motor,:OFF @14:56:51.18 Gate.power :core,:OFF @14:56:51.19 Gate.power :analytic1,:OFF @14:56:51.21 Gate.power :analytic2,:OFF @14:56:51.22 Gate.power :main,:OFF @14:56:51.23 Safely stopped and ready to power off @14:56:51.71 Cartridge::None in MAIN -- Not enough standard cartridges remain @16:07:54.51PDT25-Jul-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @16:07:56.10 >BREAK< ESP3 Gateway v1.42 for core bus @16:07:56.13 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:07:59.43 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @16:08:00.02 Sea pressure (Abs) tare is 6% of full scale @16:08:02.05 Polling Can every 2:00 @16:08:02.10 Loading /home/esp/esp2/bin/processAll mission @16:08:02.34 Sea pressure (Abs) tare is 6% of full scale @16:08:02.35 Sampling default goal volumes @16:08:02.38 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @16:08:02.57 Gate.power :sampler,:OFF,limit to 3500mA @16:08:02.62 Gate.power :analytic1,:ON,limit to 5000mA @16:08:02.63 Gate.power :analytic2,:ON,limit to 5000mA @16:08:03.60 SPR Module 1, FW Rev. B, FPGA Rev. C @16:08:03.73
Archive.configure :default @16:08:03.89 Air.configure :default @16:08:04.02 Lysis1.configure :default @16:08:04.15 Lysis2.configure :default @16:08:04.30 Plunge.configure :default @16:08:04.47 SamplePump.configure :default @16:08:04.65 Toroid.configure :default @16:08:04.71 Toroid.hold @16:08:04.78 Toroid.configure :default @16:08:04.94 Carousel.configure :default @16:08:05.00 Carousel.hold @16:08:05.08 Carousel.configure :default @16:08:05.24 Pressure.configure :default @16:08:05.40 ExtraPump.configure :default @16:08:05.52 PinPort.configure :default @16:08:05.59 All elf controllers running firmware version 4.84 @16:08:06.41 Toroid.seek :clear @16:08:06.49 Carousel.in :default @16:08:06.65 Carousel.home.seek 44 @16:08:06.91 Selecting Cartridge 43 @16:08:07.22 Carousel.seek 43 @16:08:07.76 Lysis1.in :fast @16:08:07.82 Lysis1.seek :locked @16:08:10.44 Plunge.seek :locked @16:08:10.64 Air.in :fast @16:08:10.70 Air.seek :locked @16:08:12.27
Carousel.reconfigure :locked @16:08:12.45 Powering cartridge bus @16:08:15.10 Gate.setIR 1750,5000,0,6 @16:08:15.22 Gate.setIR 0,5000,0,6 @16:08:15.29 PuckHeater.configure :default @16:08:15.36 Gate.power :heat,:ON,limit to 5000mA @16:08:16.04 Mounted lysis Cartridge @16:08:16.06 WARNING: Using default puck temperature sensor calibration @16:08:16.08 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:08:18.15 SPR.command "read_sequences" @16:08:18.63 SPR.command "stats_on" @16:08:18.81 SPR.command "start 0 1" @16:08:19.50 SPR.start /var/log/esp/spr23jul2516h.spr.bz2 @16:08:21.48 Waiting for baseline SPR stabilization @16:08:21.51 Handoff.select :waste @16:08:28.21 Syringe tare is 19% of full scale @16:08:28.24 PinPort.in :locking @16:08:28.30 PinPort.seek :locked @16:08:30.84 Archive.in :fast @16:08:30.92 Lysis2.in :fast @16:08:30.95 Lysis1.seek 1000uL @16:08:31.12 Archive.seek :full @16:08:31.19 Lysis2.seek 1000uL @16:08:35.73 delayUntil "16:18:21.02" @16:18:21.04 Lysis2.in :DAmix @16:18:21.17 Lysis1.in :DAmix @16:18:21.70 Lysis2.seek 750uL @16:18:21.74 Lysis1.seek 750uL @16:18:56.94 delay 5 seconds @16:19:01.96 Handoff.select SPR @16:19:02.09 SPR.command "start 2 14" @16:19:02.76 SPR stabilized @16:19:02.93 delay 10 seconds @16:19:12.96 SPR.command "start 2 0" @16:19:16.12 Waiting up to 11:00 for SPR to enter state 1 @16:21:13.77 SPR.command "hold_on" @16:21:15.67 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:21:16.25 Lysis2.seek 450uL @16:21:16.32 Lysis1.seek 450uL @16:21:58.32 delay 15 seconds @16:22:13.35 SPR.command "hold_off" @16:22:16.78 Waiting up to 38:00 for SPR to enter state 7 @16:36:35.98 SPRsummary:19.7RIU,104RIU,147RIU,244RIU after 1791s @16:48:06.60 delay 10 seconds @16:48:16.63 SPR.command "start 2 0" @16:48:19.29 Waiting up to 11:00 for SPR to enter state 1 @16:50:16.94 SPR.command "hold_on" @16:50:18.34 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:50:18.96 Lysis2.seek 300uL @16:50:19.01 Lysis1.seek 300uL @16:50:40.12 delay 15 seconds @16:50:55.14 SPR.command "hold_off" @16:50:58.19 Waiting up to 38:00 for SPR to enter state 7 @17:16:48.94 delay 10 seconds @17:16:58.96 SPR.command "start 2 0" @17:17:01.65 Waiting up to 11:00 for SPR to enter state 1 @17:18:59.31 SPR.command "hold_on" @17:19:00.70 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:19:01.72 Lysis2.seek 150uL @17:19:01.77 Lysis1.seek 150uL @17:19:22.84 delay 15 seconds @17:19:37.86 SPR.command "hold_off" @17:19:41.06 Waiting up to 38:00 for SPR to enter state 7 @17:45:32.24 delay 10 seconds @17:45:42.27 SPR.command "start 2 0" @17:45:45.48 Waiting up to 11:00 for SPR to enter state 1 @17:47:43.57 SPR.command "hold_on" @17:47:44.95 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:47:45.63 Lysis2.seek 0uL @17:47:45.68 Lysis1.seek 0uL @17:48:06.77 delay 15 seconds @17:48:21.80 SPR.command "hold_off" @17:48:24.80 Waiting up to 38:00 for SPR to enter state 7 @18:14:15.41 SPR analysis completed @18:14:19.06 Ended log spr23jul2516h.spr.bz2 @18:14:19.08 SPR.command "stats_off" @18:14:19.91 Intake.close @18:14:19.97 Exhaust.close @18:14:20.05 Bypass.close @18:14:20.12 Handoff.select :close @18:14:20.36
Gate.power :heat,:OFF @18:14:20.37 Gate.power :cartridge,:OFF @18:14:20.46 PinPort.in :unlocking @18:14:20.55 PinPort.seek :clear @18:14:22.48 Carousel.in :default @18:14:22.64 Carousel.home.seek 43 @18:14:23.33 Plunge.seek :locked @18:14:23.52 Archive.in :unlocking @18:14:23.68 Archive.seek :clear @18:14:23.78 Air.in :unlocking @18:14:23.97 Air.seek :clear @18:14:24.01 Lysis1.in :unlocking @18:14:24.22 Lysis1.seek :clear @18:14:24.28 Lysis2.in :unlocking @18:14:24.42 Lysis2.seek :clear @18:14:29.02
Gate.power :motor,:OFF @18:14:29.03 Gate.power :core,:OFF @18:14:29.04 Gate.power :analytic1,:OFF @18:14:29.06 Gate.power :analytic2,:OFF @18:14:29.07 Gate.power :main,:OFF @18:14:29.08 Safely stopped and ready to power off @18:14:29.56 Cartridge::None in MAIN -- Not enough standard cartridges remain @08:07:12.94PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @08:07:14.53 >BREAK< ESP3 Gateway boot v1.43 for core bus @08:07:14.55 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @08:07:17.98 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @08:07:18.59 Sea pressure (Abs) tare is 6% of full scale @08:07:20.96 Polling Can every 2:00 @08:07:21.00 Waiting for espclients... Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @08:08:06.29 Session Begun @08:09:12.77 Session Begun @08:09:17.16 -> fire @08:09:17.40 Gate.power :sampler,:OFF,limit to 3500mA @08:09:17.46 Gate.power :analytic1,:ON,limit to 5000mA @08:09:17.49 Gate.power :analytic2,:ON,limit to 5000mA @08:09:18.72 Archive.configure :default @08:09:18.95 Air.configure :default @08:09:19.14 Lysis1.configure :default @08:09:19.34 Lysis2.configure :default @08:09:19.56 Plunge.configure :default @08:09:19.77 SamplePump.configure :default @08:09:20.26 Toroid.configure :default @08:09:20.38 Toroid.hold @08:09:20.51 Toroid.configure :default @08:09:20.78 Carousel.configure :default @08:09:20.90 Carousel.hold @08:09:21.08 Carousel.configure :default @08:09:21.32 Pressure.configure :default @08:09:21.54 ExtraPump.configure :default @08:09:21.99 PinPort.configure :default @08:09:22.11 All elf controllers running firmware version 4.84 @08:09:23.49 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:09:23.53 AnalyticModule::Unknown in beadle -- SPR @08:09:23.71 Intake.close @08:09:23.76 Exhaust.close @08:09:23.90 Bypass.close @08:09:24.27 Carousel.in :default @08:09:24.45 Carousel.home.seek 53 @08:09:24.86 Lysis1.in :fast @08:09:24.97 Lysis1.seek :locked @08:09:27.53 Plunge.seek :locked @08:09:28.97 Lysis1.in :unlocking @08:09:29.18 Lysis1.seek :clear @08:09:30.76 Gate.power :motor,:OFF @08:09:30.78 Gate.power :core,:OFF @08:09:30.86 Gate.power :analytic1,:OFF @08:09:30.89 Gate.power :analytic2,:OFF @08:09:30.92 Gate.power :main,:OFF @08:09:30.96 Safely stopped and ready to power off :STOPPED @08:19:31.66 -> MainThread.exit # @08:19:31.80 ThreadError in n -- killed thread @08:19:31.90 Session Ended @08:19:31.94 Session Ended @08:20:56.55PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @08:20:58.15 >BREAK< ESP3 Gateway boot v1.43 for core bus @08:20:58.17 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @08:21:01.55 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @08:21:02.16 Sea pressure (Abs) tare is 6% of full scale @08:21:04.42 Polling Can every 2:00 @08:21:04.47 Waiting for espclients... nohup: {fire}: No such file or directory nohup: {fire}: No such file or directory Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @08:23:50.34 Session Begun @08:23:55.80 -> MainThread.exit # @08:23:55.92 Session Ended nohup: {fire}: No such file or directory @08:25:00.04PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @08:25:02.30 >BREAK< ESP3 Gateway boot v1.43 for core bus @08:25:02.34 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @08:25:06.31 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @08:25:06.96 Sea pressure (Abs) tare is 6% of full scale @08:25:09.28 Polling Can every 2:00 @08:25:09.33 Waiting for espclients... @08:25:10.06 Session Begun @08:25:16.73 -> fire @08:25:16.79 ready to power off :STOPPED @08:25:47.08 -> MainThread.exit # @08:25:47.20 ThreadError in beadle -- killed thread @08:25:47.27 Session Ended @08:47:51.53PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @08:47:53.23 >BREAK< ESP3 Gateway boot v1.43 for core bus @08:47:53.25 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @08:47:56.77 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @08:47:57.42 Sea pressure (Abs) tare is 6% of full scale @08:47:59.86 Polling Can every 2:00 @08:47:59.91 Waiting for espclients... @08:48:00.15 Session Begun @08:48:08.51 -> exit @08:48:08.54 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @08:51:26.36 Session Begun @08:51:35.14 -> MainThread.exit # @08:51:35.24 ThreadError in n -- killed thread @08:51:35.30 Session Ended @08:52:20.43PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @08:52:22.16 >BREAK< ESP3 Gateway boot v1.43 for core bus @08:52:22.19 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @08:52:25.71 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @08:52:26.34 Sea pressure (Abs) tare is 6% of full scale @08:52:28.87 Polling Can every 2:00 @08:52:28.93 Waiting for espclients... @08:52:30.02 Session Begun @08:52:33.14 -> fire @08:52:33.34 Gate.power :sampler,:OFF,limit to 3500mA @08:52:33.36 Gate.power :analytic1,:ON,limit to 5000mA @08:52:33.43 Gate.power :analytic2,:ON,limit to 5000mA @08:52:34.50 Archive.configure :default @08:52:34.74 Air.configure :default @08:52:34.84 Air.hold @08:52:34.96 Air.configure :default @08:52:35.13 Lysis1.configure :default @08:52:35.23 Lysis1.hold @08:52:35.33 Lysis1.configure :default @08:52:35.76 Lysis2.configure :default @08:52:35.98 Plunge.configure :default @08:52:36.08 Plunge.hold @08:52:36.19 Plunge.configure :default @08:52:36.39 SamplePump.configure :default @08:52:36.58 Toroid.configure :default @08:52:36.71 Toroid.hold @08:52:36.84 Toroid.configure :default @08:52:37.04 Carousel.configure :default @08:52:37.41 Carousel.hold @08:52:37.52 Carousel.configure :default @08:52:37.72 Pressure.configure :default @08:52:37.92 ExtraPump.configure :default @08:52:38.09 PinPort.configure :default @08:52:38.21 All elf controllers running firmware version 4.84 @08:52:38.91 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:52:38.95 AnalyticModule::Unknown in nadia -- SPR @08:52:39.10 Intake.close @08:52:39.17 Exhaust.close @08:52:39.32 Bypass.close @08:52:39.58 Carousel.in :default @08:52:39.75 Carousel.home.seek 33 @08:52:40.11 Plunge.seek :locked @08:52:40.42 Air.in :unlocking @08:52:40.61 Air.seek :clear @08:52:40.71 Lysis1.in :unlocking @08:52:41.00 Lysis1.seek :clear @08:52:42.52 Gate.power :motor,:OFF @08:52:42.54 Gate.power :core,:OFF @08:52:42.58 Gate.power :analytic1,:OFF @08:52:42.61 Gate.power :analytic2,:OFF @08:52:42.67 Gate.power :main,:OFF @08:52:42.71 Safely stopped and ready to power off :STOPPED @09:05:51.14 -> Power.sampler :ON @09:05:51.19 Gate.power :sampler,:ON true @09:06:13.06 -> ESP.ready! @09:06:13.12 Gate.power :sampler,:OFF @09:06:13.14 Gate.power :sampler,:OFF,limit to 3500mA @09:06:13.17 Gate.power :main,:ON,limit to 5000mA @09:06:13.21 Gate.power :analytic1,:ON,limit to 5000mA @09:06:13.26 Gate.power :analytic2,:ON,limit to 5000mA @09:06:13.36 Powering core bus @09:06:16.14 Archive.configure :default @09:06:16.26 Air.configure :default @09:06:16.36 Lysis1.configure :default @09:06:16.46 Lysis2.configure :default @09:06:16.58 Plunge.configure :default @09:06:16.74 SamplePump.configure :default @09:06:16.87 Toroid.configure :default @09:06:17.79 Carousel.configure :default @09:06:17.92 Pressure.configure :default @09:06:18.04 ExtraPump.configure :default @09:06:18.15 PinPort.configure :default @09:06:18.24 All elf controllers running firmware version 4.84 @09:06:18.27 Gate.power :motor,:ON,limit to 5000mA @09:06:20.92 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @09:06:20.95 AnalyticModule::Unknown in nadia -- SPR @09:06:21.37 Archive.home.jog 0 @09:06:21.98 PinPort.home.jog 0 @09:06:22.04 Toroid.home.jog 0 @09:06:22.09 Plunge.home.jog 0 @09:06:22.13 Lysis1.home.jog 0 @09:06:22.18 Air.home.jog 0 @09:06:22.23 Lysis2.home.jog 0 @09:06:22.30 Carousel.home.jog 0 @09:06:23.22 Archive.in :homing @09:06:23.56 Archive.jog 1383 @09:06:23.65 Toroid.in :homing @09:06:23.71 Lysis1.in :homing @09:06:23.78 PinPort.in :homing @09:06:23.82 Lysis2.in :homing @09:06:23.90 Plunge.in :homing @09:06:23.95 Air.in :homing @09:06:24.32 Toroid.jog 607 @09:06:24.36 PinPort.jog -110 @09:06:24.41 Lysis1.jog 1385 @09:06:24.45 Lysis2.jog 1392 @09:06:24.51 Plunge.jog 1557 @09:06:24.55 Air.jog 1362 @09:06:25.07 PinPort.setRawPosition! 0 @09:06:25.15 PinPort.seek :home @09:06:25.22 Archive.setRawPosition! 50 @09:06:25.38 Archive.seek :home @09:06:25.51 PinPort.in :default @09:06:25.69 Archive.in :default @09:06:25.97 Lysis1.setRawPosition! 50 @09:06:26.11 Lysis2.setRawPosition! 50 @09:06:26.14 Air.setRawPosition! 50 @09:06:26.19 Lysis1.seek :home @09:06:26.38 Lysis2.seek :home @09:06:26.42 Air.seek :home @09:06:26.71 Plunge.setRawPosition! 50 @09:06:26.76 Lysis1.in :default @09:06:26.87 Plunge.seek :home @09:06:27.10 Air.in :default @09:06:27.14 Toroid.setRawPosition! 0 @09:06:27.19 Lysis2.in :default @09:06:27.36 Toroid.seek :home @09:06:27.54 Plunge.in :default @09:06:27.69 Toroid.in :default @09:06:27.92 Toroid.seek :clear @09:06:29.56 Carousel.in :default @09:06:29.73 Carousel.home.seek 33 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 33 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @09:06:33.13 -> Power.sampler :ON @09:06:33.17 Gate.power :sampler,:ON true @09:07:02.03 -> BACsampler.prime @09:07:02.15 Priming sample loop w/100ml, bypass w/10ml @09:07:02.17 SamplePump.setPosition! 0ml @09:07:02.27 Bypass.open @09:07:03.82 Intake.open @09:07:04.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:07:07.19 Vacuum tare is 55% of full scale @09:07:07.39 Pressure.in :default @09:07:07.48 Pressure.hold @09:07:07.76 SamplePump.in :prime @09:07:07.86 SamplePump.seek 10ml,59 seconds @09:07:20.32 Pressure.stop @09:07:20.40 SamplePump.setPosition! 0ml @09:07:20.47 Exhaust.open @09:07:21.98 Bypass.close @09:07:23.56 Pressure.in :default @09:07:23.65 Pressure.hold @09:07:23.99 SamplePump.in :prime @09:07:24.10 SamplePump.seek 100ml,5:16 @09:09:25.79 Pressure.stop BACsampler @09:35:16.94 -> Power.sampler :OFF @09:35:16.96 Gate.power :sampler,:OFF false @09:46:06.38 Session Begun @09:46:08.60 -> slots {33 => { Type: :archiveNull_bac, State: :dry}} @09:46:10.70 -> exit @09:46:10.73 Session Ended @09:59:28.66 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:10:52.19 Session Begun @11:11:00.11 -> slots {33 => { Type: :archiveNull_bac, State: :dry}} @11:11:52.10 -> fire :archiveNull_bac @11:11:53.03 Toroid.seek :clear @11:11:53.15 Carousel.in :default @11:11:53.31 Carousel.home.seek 33 @11:11:53.62 Selecting Cartridge 33 @11:11:53.97 Carousel.seek 33 @11:11:54.27 Lysis1.in :fast @11:11:54.38 Lysis1.seek :locked @11:11:57.03 Plunge.seek :locked @11:11:57.75 Air.in :fast @11:11:57.85 Air.seek :locked @11:11:59.45 Carousel.reconfigure :locked @11:11:59.66 Powering cartridge bus @11:12:02.35 Gate.power :cartridge,:OFF @11:12:02.94 Mounted archive Cartridge @11:12:03.35 Preparing to filter cartridge 33 @11:12:03.63 Gate.power :sampler,:ON @11:12:03.68 Bypass.open @11:12:04.47 Intake.close @11:12:05.30 Toroid.in :hold @11:12:05.40 Toroid.seek :crack @11:12:06.97 Toroid.seek :cartridge @11:12:08.11 Toroid.in :default @11:12:08.22 Toroid.seek :clear @11:12:10.28 Pressurizing cartridge to 26psi above 13.2psia @11:12:10.30 SamplePump.setPosition! 0ml @11:12:10.38 Intake.open @11:12:11.12 Bypass.close @11:12:11.81 Exhaust.close @11:12:12.72 Pressure.in :default @11:12:12.82 Pressure.hold @11:12:13.09 SamplePump.in :charge @11:12:13.21 SamplePump.seek 2.3ml,42 seconds @11:12:23.57 Pressure.stop @11:12:23.66 Intake.close @11:12:24.47 Pressurized cartridge with 0.480ml to 26.2psi @11:12:24.49 Isolated @31.4psia (18.2psi) @11:12:24.52 Verifying cartridge holds at least 0.65..0.85 of 31.4psia @11:12:24.58 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:12:29.21 Cartridge pressure fell 8% to 28.9psia @11:12:29.34 Sampling 1000.0ml @11:12:29.36 SamplePump.setPosition! 0ml @11:12:29.47 Exhaust.open @11:12:30.24 Intake.open @11:12:31.11 Pressure.in :default @11:12:31.21 Pressure.hold @11:12:31.38 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:12:31.59 Waiting for external sampler to finish priming... @11:12:31.60 delayUntil "11:17:03.65" @11:13:09.67 Session Begun @11:13:18.09 -> showlog -30 @11:14:04.91 -> Sampler::PumpPrimeDelay 5:00 @11:17:03.72 SamplePump.in :sample @11:17:03.85 SamplePump.seek 1000ml,1:23:51 @11:17:06.88 Cartridge 33 filtering 1/1000ml at 0.61ml/s with 25.7psi vacuum @11:18:06.86 Cartridge 33 filtering 37/1000ml at 0.60ml/s with 16.9psi vacuum 2.4m deep (18.2psia) @11:18:30.46 -> MainThread.exit @11:18:30.52
SamplePump.stop # @11:18:30.78 SamplePump.stop @11:18:30.93 Intake.close @11:18:31.06 Exhaust.close @11:18:31.31
SamplePump.stop @11:18:31.52 Toroid.in :hold @11:18:31.65 Toroid.seek :bypass @11:18:31.79 Intake.close @11:18:31.97 Exhaust.close @11:18:32.16 Session Ended @11:18:32.25 Session Ended @11:18:32.33 Toroid.in :default @11:18:32.42 Pressure.stop @11:19:20.60PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @11:19:22.27 >BREAK< ESP3 Gateway boot v1.43 for core bus @11:19:22.29 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @11:19:25.89 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @11:19:27.12 Sea pressure (Abs) tare is 6% of full scale @11:19:29.48 Polling Can every 2:00 @11:19:29.55 Waiting for espclients... @11:19:30.23 Session Begun @11:19:34.38 -> ESP.shutdown @11:19:34.44 Gate.power :motor,:OFF @11:19:34.45 Gate.power :core,:OFF @11:19:34.48 Gate.power :sampler,:OFF @11:19:34.52 Gate.power :analytic1,:OFF @11:19:34.55 Gate.power :analytic2,:OFF @11:19:34.58 Gate.power :main,:OFF [:motor,:core,:sampler,:analytic1,:analytic2,:main] @11:20:17.90 -> fire :archiveNull_bac @11:20:17.93 Cartridge::None in n -- No dry archiveNull_bac cartridges remain @11:20:48.30 -> slots 33=>:dry {33 => { Type: :archiveNull_bac, State: :dry, TV: :"?"}} @11:20:50.16 -> slots {33 => { Type: :archiveNull_bac, State: :dry, TV: :"?"}} @11:20:54.49 -> TV.goHome! @11:20:54.52 Axis::Error in n -- Toroid Valve (TV) has no homing configuration @11:21:00.86 -> ESP.reready! @11:21:00.91 Gate.power :sampler,:OFF,limit to 3500mA @11:21:00.93 Gate.power :main,:ON,limit to 5000mA @11:21:00.96 Gate.power :analytic1,:ON,limit to 5000mA @11:21:00.99 Gate.power :analytic2,:ON,limit to 5000mA @11:21:01.55 Powering core bus @11:21:04.37 Archive.configure :default @11:21:04.49 Air.configure :default @11:21:04.58 Lysis1.configure :default @11:21:04.70 Lysis2.configure :default @11:21:05.09 Plunge.configure :default @11:21:05.22 SamplePump.configure :default @11:21:05.34 Toroid.configure :default @11:21:05.46 Carousel.configure :default @11:21:05.58 Pressure.configure :default @11:21:05.71 ExtraPump.configure :default @11:21:05.80 PinPort.configure :default @11:21:05.91 All elf controllers running firmware version 4.84 @11:21:05.94 Gate.power :motor,:ON,limit to 5000mA @11:21:08.35 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:21:08.38 AnalyticModule::Unknown in n -- SPR @11:21:08.45 Archive.home.jog 0 @11:21:08.52 Air.home.jog 0 @11:21:08.72 Lysis1.home.jog 0 @11:21:08.82 Lysis2.home.jog 0 @11:21:08.92 Plunge.home.jog 0 @11:21:09.09 Toroid.home.jog 0 @11:21:09.16 Carousel.home.jog 0 @11:21:09.25 PinPort.home.jog 0 @11:21:09.82 Archive.in :homing @11:21:10.13 Archive.jog 1382 @11:21:10.40 Lysis1.in :homing @11:21:10.51 Plunge.in :homing @11:21:10.61 Lysis2.in :homing @11:21:10.70 Air.in :homing @11:21:10.78 Toroid.in :homing @11:21:11.02 Lysis1.jog 2134 @11:21:11.09 Plunge.jog 1558 @11:21:11.12 Lysis2.jog 1391 @11:21:11.17 Air.jog 1963 @11:21:11.22 Toroid.jog 229 @11:21:11.61 Archive.setRawPosition! 50 @11:21:11.64 PinPort.in :homing @11:21:11.79 Archive.seek :home @11:21:11.89 PinPort.jog -110 @11:21:12.12 Archive.in :default @11:21:12.23 PinPort.setRawPosition! 0 @11:21:12.32 PinPort.seek :home @11:21:12.45 PinPort.in :default @11:21:12.47 Toroid.setRawPosition! 0 @11:21:12.72 Plunge.setRawPosition! 50 @11:21:12.75 Lysis2.setRawPosition! 50 @11:21:12.79 Toroid.seek :home @11:21:12.99 Plunge.seek :home @11:21:13.03 Lysis2.seek :home @11:21:13.28 Lysis1.setRawPosition! 50 @11:21:13.31 Air.setRawPosition! 50 @11:21:13.35 Toroid.in :default @11:21:13.56 Air.seek :home @11:21:13.60 Lysis1.seek :home @11:21:13.83 Lysis2.in :default @11:21:13.85 Plunge.in :default @11:21:14.08 Air.in :default @11:21:14.10 Lysis1.in :default @11:21:14.53 Toroid.seek :clear @11:21:16.18 Carousel.in :default @11:21:16.36 Carousel.home.seek 33 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 33 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @11:21:30.62 -> fire :archiveNull_bac @11:21:31.53 Toroid.seek :clear @11:21:31.65 Carousel.in :default @11:21:31.81 Carousel.home.seek 33 @11:21:32.13 Ensuring all (1) cartridges are bypassed @11:21:32.48 Carousel.seek 33 @11:21:32.64 Toroid.in :hold @11:21:32.78 Toroid.seek :bypass @11:21:34.45 Toroid.in :default @11:21:34.55 Toroid.seek :clear @11:21:36.20 Selecting Cartridge 33 @11:21:36.56 Carousel.seek 33 @11:21:36.89 Lysis1.in :fast @11:21:36.99 Lysis1.seek :locked @11:21:39.65 Plunge.seek :locked @11:21:39.85 Air.in :fast @11:21:39.95 Air.seek :locked @11:21:41.54 Carousel.reconfigure :locked @11:21:41.75 Powering cartridge bus @11:21:44.44 Gate.power :cartridge,:OFF @11:21:45.00 Mounted archive Cartridge @11:21:45.39 Preparing to filter cartridge 33 @11:21:45.67 Gate.power :sampler,:ON @11:21:45.79 Priming sample loop w/100ml, bypass w/10ml @11:21:45.82 SamplePump.setPosition! 0ml @11:21:45.89 Bypass.open @11:21:47.40 Intake.open @11:21:48.19 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:21:50.91 Vacuum tare is 55% of full scale @11:21:51.10 Pressure.in :default @11:21:51.18 Pressure.hold @11:21:51.44 SamplePump.in :prime @11:21:51.56 SamplePump.seek 10ml,59 seconds @11:22:04.02 Pressure.stop @11:22:04.15 SamplePump.setPosition! 0ml @11:22:04.22 Exhaust.open @11:22:05.69 Bypass.close @11:22:07.27 Pressure.in :default @11:22:07.37 Pressure.hold @11:22:07.70 SamplePump.in :prime @11:22:07.81 SamplePump.seek 100ml,5:16 @11:22:24.32 SamplePump.stop @11:22:24.47 Intake.close @11:22:24.58 Exhaust.close @11:22:25.45 Pressure.stop @11:22:25.66 Cmd::Paused in FILTERING -- paused by n during Sample Pump (SP) move after sampling 0.0ml @11:22:25.70 UserAbort in n -- User Abort!! @11:22:28.66 Gate.power :sampler,:OFF @11:23:20.21 -> fire :archiveNull_bac @11:23:20.25 Selecting Cartridge 33 @11:23:21.07 Preparing to filter cartridge 33 @11:23:21.36 Gate.power :sampler,:ON @11:23:21.49 Priming sample loop w/100ml, bypass w/10ml @11:23:21.52 SamplePump.setPosition! 0ml @11:23:21.61 Bypass.open @11:23:23.13 Intake.open @11:23:23.90 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:23:26.49 Vacuum tare is 56% of full scale @11:23:26.72 Pressure.in :default @11:23:26.81 Pressure.hold @11:23:27.08 SamplePump.in :prime @11:23:27.18 SamplePump.seek 10ml,59 seconds @11:23:39.62 Pressure.stop @11:23:39.71 SamplePump.setPosition! 0ml @11:23:39.78 Exhaust.open @11:23:41.25 Bypass.close @11:23:43.52 Pressure.in :default @11:23:43.69 Pressure.hold @11:23:44.24 Session Begun @11:23:44.60 SamplePump.in :prime @11:23:44.86 SamplePump.seek 100ml,5:16 @11:23:50.70 -> ESP.shutdown @11:23:50.73 SamplePump.stop @11:23:50.94 SamplePump.stop @11:23:51.12 Intake.close @11:23:51.21 Exhaust.close @11:23:51.98 Intake.close @11:23:52.13 Exhaust.close @11:23:52.16 Pressure.stop @11:23:52.45 Exhaust.close @11:23:52.72 Slide::Error in FILTERING -- Sample Pump (SP) aborted at 4.71ml @11:23:52.74 Waiting to Resume from Checkpoint "Sample Pump (SP) aborted at 4.71ml" @11:23:52.85 Gate.power :motor,:OFF @11:23:52.87 Gate.power :core,:OFF @11:23:52.93 Gate.power :sampler,:OFF @11:23:52.97 Gate.power :analytic1,:OFF @11:23:53.02 Gate.power :analytic2,:OFF @11:23:53.08 Gate.power :main,:OFF [:motor,:core,:sampler,:analytic1,:analytic2,:main] @11:23:58.88 -> MainThread.exit # @11:23:58.99 ThreadError in beadle -- killed thread @11:23:59.12 Thread::Aborted in FILTERING -- "Aborted by n" @11:23:59.18 Session Ended @11:23:59.23 Waiting to Resume from Checkpoint "Sample Pump (SP) aborted at 4.71ml" @11:23:59.29 ThreadError in FILTERING -- killed thread @11:23:59.33 Waiting to Resume from Checkpoint "Sample Pump (SP) aborted at 4.71ml" @11:23:59.38 Session Ended @12:27:30.24PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @12:27:34.10 >BREAK< ESP3 Gateway boot v1.43 for core bus @12:27:34.13 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @12:27:37.64 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @12:27:38.25 Sea pressure (Abs) tare is 6% of full scale @12:27:40.94 Polling Can every 2:00 @12:27:41.10 Waiting for espclients... @12:27:41.59 Session Begun @12:27:51.83 -> ESP.ready! @12:27:51.90 Gate.power :sampler,:OFF,limit to 3500mA @12:27:51.92 Gate.power :main,:ON,limit to 5000mA @12:27:51.95 Gate.power :analytic1,:ON,limit to 5000mA @12:27:51.98 Gate.power :analytic2,:ON,limit to 5000mA @12:27:52.16 Powering core bus @12:27:54.94 Archive.configure :default @12:27:55.06 Air.configure :default @12:27:55.15 Lysis1.configure :default @12:27:55.25 Lysis2.configure :default @12:27:55.36 Plunge.configure :default @12:27:55.48 SamplePump.configure :default @12:27:55.60 Toroid.configure :default @12:27:55.72 Carousel.configure :default @12:27:55.85 Pressure.configure :default @12:27:55.97 ExtraPump.configure :default @12:27:56.06 PinPort.configure :default @12:27:56.15 All elf controllers running firmware version 4.84 @12:27:56.20 Gate.power :motor,:ON,limit to 5000mA @12:27:59.76 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:27:59.79 AnalyticModule::Unknown in errera -- SPR @12:27:59.99 Archive.home.jog 0 @12:28:00.92 PinPort.home.jog 0 @12:28:00.97 Toroid.home.jog 0 @12:28:01.02 Plunge.home.jog 0 @12:28:01.06 Lysis1.home.jog 0 @12:28:01.11 Air.home.jog 0 @12:28:01.14 Carousel.home.jog 0 @12:28:01.19 Lysis2.home.jog 0 @12:28:01.99 Archive.in :homing @12:28:02.38 Archive.jog 1385 @12:28:02.51 PinPort.in :homing @12:28:02.56 Lysis2.in :homing @12:28:02.60 Air.in :homing @12:28:02.69 Plunge.in :homing @12:28:02.73 Toroid.in :homing @12:28:02.87 Lysis1.in :homing @12:28:03.12 Lysis2.jog 1393 @12:28:03.17 PinPort.jog -111 @12:28:03.27 Air.jog 1958 @12:28:03.31 Plunge.jog 1556 @12:28:03.39 Toroid.jog 600 @12:28:03.45 Lysis1.jog 2132 @12:28:03.76 PinPort.setRawPosition! 0 @12:28:03.84 PinPort.seek :home @12:28:03.98 PinPort.in :default @12:28:04.09 Archive.setRawPosition! 50 @12:28:04.15 Archive.seek :home @12:28:04.47 Archive.in :default @12:28:04.58 Lysis2.setRawPosition! 50 @12:28:04.65 Lysis2.seek :home @12:28:04.83 Plunge.setRawPosition! 50 @12:28:04.90 Plunge.seek :home @12:28:05.05 Air.setRawPosition! 50 @12:28:05.08 Lysis2.in :default @12:28:05.24 Air.seek :home @12:28:05.38 Plunge.in :default @12:28:05.41 Lysis1.setRawPosition! 50 @12:28:05.56 Lysis1.seek :home @12:28:05.69 Air.in :default @12:28:05.72 Toroid.setRawPosition! 0 @12:28:05.87 Toroid.seek :home @12:28:06.02 Lysis1.in :default @12:28:06.05 Toroid.in :default @12:28:06.45 Toroid.seek :clear @12:28:08.09 Carousel.in :default @12:28:08.25 Carousel.home.seek 33 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 33 Pressure = Absolute Pressure (Leak) Sensor @18psia PinPort = Handoff Pin Port (PP) clear @12:28:38.97 Session Begun @12:29:08.79 -> slots {33 => { Type: :archiveNull_bac, State: :dry}} @12:31:35.50 -> fire :archiveHiBiomass_bac, :microSPR_phyto @12:31:35.56 Cartridge::None in errera -- No dry archiveHiBiomass_bac cartridges remain @12:32:09.34 -> log.comment 'Can' @12:32:09.36 Can @12:32:19.10 -> Can Can@12:31:40, 22.1C, 36% humidity, 14.3psia, 14.870V, 0.218A, 0.024Ah, 3.24W @12:33:25.99 -> fire :archiveNull_bac @12:33:26.96 Toroid.seek :clear @12:33:27.09 Carousel.in :default @12:33:27.27 Carousel.home.seek 33 @12:33:27.60 Selecting Cartridge 33 @12:33:27.97 Carousel.seek 33 @12:33:28.28 Lysis1.in :fast @12:33:28.47 Lysis1.seek :locked @12:33:31.16 Plunge.seek :locked @12:33:31.38 Air.in :fast @12:33:31.50 Air.seek :locked @12:33:33.12 Carousel.reconfigure :locked @12:33:33.33 Powering cartridge bus @12:33:36.03 Gate.power :cartridge,:OFF @12:33:36.63 Mounted archive Cartridge @12:33:37.03 Preparing to filter cartridge 33 @12:33:37.33 Gate.power :sampler,:ON @12:33:37.48 Priming sample loop w/100ml, bypass w/10ml @12:33:37.50 SamplePump.setPosition! 0ml @12:33:37.61 Bypass.open @12:33:39.21 Intake.open @12:33:40.01 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:33:42.60 Vacuum tare is 56% of full scale @12:33:42.81 Pressure.in :default @12:33:42.91 Pressure.hold @12:33:43.70 SamplePump.in :prime @12:33:43.82 SamplePump.seek 10ml,59 seconds @12:33:54.33 -> slots {33 => { Type: :archiveNull_bac, State: :dry}} @12:33:56.29 Pressure.stop @12:33:56.42 SamplePump.setPosition! 0ml @12:33:56.52 Exhaust.open @12:33:56.90 -> slot {33 => { Type: :archiveNull_bac, State: :dry}} @12:33:58.01 Bypass.close @12:33:59.63 Pressure.in :default @12:33:59.74 Pressure.hold @12:34:00.10 SamplePump.in :prime @12:34:00.23 SamplePump.seek 100ml,5:16 @12:34:03.39 SamplePump.stop @12:34:03.50 Intake.close @12:34:03.59 Exhaust.close @12:34:04.46 Pressure.stop @12:34:04.78 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 18.4psi vacuum after 2.4ml @12:34:04.81 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.36ml" @12:34:07.67 Gate.power :sampler,:OFF @12:34:43.32 -> MainThread.resume @12:34:43.35 Thread::CannotResume in n -- MAIN is not suspended @12:34:48.57 -> MainThread.recover @12:34:48.64 Thread::CannotResume in n -- No recoverable exceptions @12:35:18.53 -> Power.sampler :ON @12:35:18.56 Gate.power :sampler,:ON true @12:35:45.41 Cmd::Error in errera -- Not Filtering @12:35:45.49 UserAbort in errera -- User Abort!! @12:36:02.15 -> fire :archiveNull_bac @12:36:02.22 Cartridge::None in errera -- No dry archiveNull_bac cartridges remain @12:36:18.46 -> slot 33=> :dry {33 => { Type: :archiveNull_bac, State: :dry}} @12:36:21.00 -> slots {33 => { Type: :archiveNull_bac, State: :dry}} @12:36:29.49 -> fire :archiveNull_bac @12:36:29.54 Selecting Cartridge 33 @12:36:30.35 Preparing to filter cartridge 33 @12:36:30.77 Priming sample loop w/100ml, bypass w/10ml @12:36:30.80 SamplePump.setPosition! 0ml @12:36:30.91 Bypass.open @12:36:32.48 Intake.open @12:36:33.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:36:35.85 Vacuum tare is 56% of full scale @12:36:36.06 Pressure.in :default @12:36:36.17 Pressure.hold @12:36:36.49 SamplePump.in :prime @12:36:36.62 SamplePump.seek 10ml,59 seconds @12:36:49.07 Pressure.stop @12:36:49.17 SamplePump.setPosition! 0ml @12:36:49.26 Exhaust.open @12:36:50.71 Bypass.close @12:36:52.30 Pressure.in :default @12:36:52.45 Pressure.hold @12:36:53.31 SamplePump.in :prime @12:36:53.43 SamplePump.seek 100ml,5:16 @12:38:55.14 Pressure.stop @12:38:55.26 Bypass.open @12:38:56.02 Intake.close @12:38:56.85 Toroid.in :hold @12:38:56.96 Toroid.seek :crack @12:38:58.55 Toroid.seek :cartridge @12:38:59.71 Toroid.in :default @12:38:59.81 Toroid.seek :clear @12:39:01.89 Pressurizing cartridge to 26psi above 13.2psia @12:39:01.91 SamplePump.setPosition! 0ml @12:39:02.02 Intake.open @12:39:02.73 Bypass.close @12:39:03.48 Exhaust.close @12:39:04.36 Pressure.in :default @12:39:04.50 Pressure.hold @12:39:04.78 SamplePump.in :charge @12:39:04.90 SamplePump.seek 2.3ml,42 seconds @12:39:16.23 Pressure.stop @12:39:16.34 Intake.close @12:39:17.18 Pressurized cartridge with 0.574ml to 26.3psi @12:39:17.20 Isolated @32.0psia (18.8psi) @12:39:17.25 Verifying cartridge holds at least 0.65..0.85 of 32.0psia @12:39:17.30 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:39:21.94 Cartridge pressure fell 6% to 30.1psia @12:39:22.09 Sampling 1000.0ml @12:39:22.10 SamplePump.setPosition! 0ml @12:39:22.22 Exhaust.open @12:39:22.98 Intake.open @12:39:23.85 Pressure.in :default @12:39:23.95 Pressure.hold @12:39:24.15 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:39:24.36 Waiting for external sampler to finish priming... @12:39:24.42 delayUntil "12:40:18.61" @12:40:18.67 SamplePump.in :sample @12:40:18.78 SamplePump.seek 1000ml,1:23:51 @12:40:21.81 Cartridge 33 filtering 1/1000ml at 0.61ml/s with 20.3psi vacuum @12:41:21.82 Cartridge 33 filtering 38/1000ml at 0.63ml/s with 23.5psi vacuum @12:42:21.82 Cartridge 33 filtering 74/1000ml at 0.62ml/s with 25.5psi vacuum @12:43:22.35 Cartridge 33 filtering 110/1000ml at 0.61ml/s with 20.8psi vacuum @12:43:38.81 Session Begun @12:43:52.06 -> Sampler.primeDelay @12:43:52.09 NoMethodError in brent -- undefined method `primeDelay' for Cartridge::Sampler:Class @12:44:21.82 Cartridge 33 filtering 145/1000ml at 0.61ml/s with 25.1psi vacuum @12:45:21.82 Cartridge 33 filtering 180/1000ml at 0.61ml/s with 20.5psi vacuum @12:46:21.41 Session Ended @12:46:21.80 Cartridge 33 filtering 215/1000ml at 0.53ml/s with 26.6psi vacuum @12:47:21.82 Cartridge 33 filtering 250/1000ml at 0.61ml/s with 26.0psi vacuum @12:48:21.81 Cartridge 33 filtering 285/1000ml at 0.58ml/s with 21.2psi vacuum @12:48:53.90 -> Cart.sampler.primeDelay 5:00 @12:49:21.81 Cartridge 33 filtering 320/1000ml at 0.59ml/s with 19.5psi vacuum @12:50:16.38 -> quit @12:50:16.43 Session Ended @12:50:21.83 Cartridge 33 filtering 355/1000ml at 0.61ml/s with 25.3psi vacuum @12:51:00.66 SamplePump.stop @12:51:00.83 Intake.close @12:51:00.91 Exhaust.close @12:51:01.24 Toroid.in :hold @12:51:01.35 Toroid.seek :bypass @12:51:03.02 Toroid.in :default @12:51:03.13 Toroid.seek :clear @12:51:04.79 Pressure.stop @12:51:05.23 Cmd::Paused in FILTERING -- paused by errera during Sample Pump (SP) move after sampling 377.9ml @12:51:05.30 UserAbort in errera -- User Abort!! @12:51:08.21 Gate.power :sampler,:OFF @12:53:23.24 -> exit @12:53:23.27 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @13:14:08.35 Session Begun @13:14:17.99 -> slots {33 => { Type: :archiveNull_bac, State: :paused, filtered: 377.9}} @13:14:31.14 -> slot 33=>:dry {33 => { Type: :archiveNull_bac, State: :dry, filtered: 377.9}} @13:14:43.67 -> slots 1..60=>nil {60..1 => nil} @13:14:51.12 -> slots 33=>:dry {33 => { Type: :unknown, State: :dry}} @13:15:01.46 -> slots 33=>:archiveNull_bac {33 => { Type: :archiveNull_bac, State: :dry}} @13:15:10.47 -> fire :archiveNull_bac @13:15:10.52 Selecting Cartridge 33 @13:15:11.29 Preparing to filter cartridge 33 @13:15:11.59 Gate.power :sampler,:ON @13:15:11.72 Priming sample loop w/100ml, bypass w/10ml @13:15:11.73 SamplePump.setPosition! 0ml @13:15:11.82 Bypass.open @13:15:13.38 Intake.open @13:15:14.15 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:15:16.74 Vacuum tare is 55% of full scale @13:15:16.94 Pressure.in :default @13:15:17.03 Pressure.hold @13:15:17.83 SamplePump.in :prime @13:15:17.93 SamplePump.seek 10ml,59 seconds @13:15:30.41 Pressure.stop @13:15:30.50 SamplePump.setPosition! 0ml @13:15:30.57 Exhaust.open @13:15:32.03 Bypass.close @13:15:33.59 Pressure.in :default @13:15:33.68 Pressure.hold @13:15:34.02 SamplePump.in :prime @13:15:34.12 SamplePump.seek 100ml,5:16 @13:15:37.25 SamplePump.stop @13:15:37.34 Intake.close @13:15:37.44 Exhaust.close @13:15:38.29 Pressure.stop @13:15:38.66 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 10.4psi vacuum after 2.3ml @13:15:38.68 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.34ml" @13:15:41.51 Gate.power :sampler,:OFF @13:15:52.30 Cmd::Error in n -- Not Filtering @13:15:52.35 UserAbort in n -- User Abort!! @13:15:56.79 -> fire :archiveNull_bac @13:15:56.83 Cartridge::None in n -- No dry archiveNull_bac cartridges remain @13:16:12.47 -> slots 33=>[:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @13:16:16.40 -> fire :archiveNull_bac @13:16:16.46 Selecting Cartridge 33 @13:16:17.21 Preparing to filter cartridge 33 @13:16:17.49 Gate.power :sampler,:ON @13:16:17.63 Priming sample loop w/100ml, bypass w/10ml @13:16:17.65 SamplePump.setPosition! 0ml @13:16:17.73 Bypass.open @13:16:19.26 Intake.open @13:16:20.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:16:23.12 Vacuum tare is 56% of full scale @13:16:23.32 Pressure.in :default @13:16:23.41 Pressure.hold @13:16:23.68 SamplePump.in :prime @13:16:23.77 SamplePump.seek 10ml,59 seconds @13:16:33.88 SamplePump.stop @13:16:33.97 Intake.close @13:16:34.04 Bypass.close @13:16:34.89 Pressure.stop @13:16:35.73 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only -0.2psi vacuum after 8.1ml @13:16:35.75 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 8.08ml" @13:16:38.07 Gate.power :sampler,:OFF @13:16:49.37 Cmd::Error in n -- Not Filtering @13:16:49.45 UserAbort in n -- User Abort!! @13:17:01.89 -> Sampler::PumpPrimeDelay 5:00 @13:17:13.91 -> MainThread.exit # @13:17:14.11 ThreadError in n -- killed thread @13:17:14.20 Thread::Aborted in FILTERING -- "Aborted by n" @13:17:14.22 Session Ended @13:17:14.24 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 8.08ml" @13:17:14.28 ThreadError in FILTERING -- killed thread @13:17:14.30 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 8.08ml" @13:17:25.83PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @13:17:27.45 >BREAK< ESP3 Gateway boot v1.43 for core bus @13:17:27.48 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @13:17:31.02 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @13:17:31.71 Sea pressure (Abs) tare is 6% of full scale @13:17:34.17 Polling Can every 2:00 @13:17:34.24 Waiting for espclients... @13:17:34.93 Session Begun @13:17:41.28 -> slots 33=>:dry {33 => { Type: :archiveNull_bac, State: :dry, filtered: 377.9}} @13:17:58.72 -> slots 33=>[:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @13:18:05.91 -> fire :archiveNull_bac @13:18:06.15 Gate.power :sampler,:OFF,limit to 3500mA @13:18:06.17 Gate.power :analytic1,:ON,limit to 5000mA @13:18:06.61 Gate.power :analytic2,:ON,limit to 5000mA @13:18:07.34 Archive.configure :default @13:18:07.54 Air.configure :default @13:18:07.63 Air.hold @13:18:07.73 Air.configure :default @13:18:07.90 Lysis1.configure :default @13:18:08.00 Lysis1.hold @13:18:08.11 Lysis1.configure :default @13:18:08.28 Lysis2.configure :default @13:18:08.51 Plunge.configure :default @13:18:08.87 Plunge.hold @13:18:08.98 Plunge.configure :default @13:18:09.16 SamplePump.configure :default @13:18:09.36 Toroid.configure :default @13:18:09.46 Toroid.hold @13:18:09.56 Toroid.configure :default @13:18:09.75 Carousel.configure :default @13:18:09.84 Carousel.hold @13:18:09.96 Carousel.configure :default @13:18:10.15 Pressure.configure :default @13:18:10.34 ExtraPump.configure :default @13:18:10.78 PinPort.configure :default @13:18:10.88 All elf controllers running firmware version 4.84 @13:18:12.15 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:18:12.20 AnalyticModule::Unknown in n -- SPR @13:18:13.09 Toroid.seek :clear @13:18:13.22 Carousel.in :default @13:18:13.40 Carousel.home.seek 33 @13:18:13.85 Carousel.reconfigure :locked @13:18:14.06 Powering cartridge bus @13:18:16.75 Gate.power :cartridge,:OFF @13:18:17.32 Mounted archive Cartridge @13:18:17.40 Selecting Cartridge 33 @13:18:18.10 Preparing to filter cartridge 33 @13:18:18.40 Gate.power :sampler,:ON @13:18:18.53 Priming sample loop w/100ml, bypass w/10ml @13:18:18.55 SamplePump.setPosition! 0ml @13:18:18.63 Exhaust.close @13:18:18.74 Bypass.open @13:18:20.77 Intake.open @13:18:21.56 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:18:24.15 Vacuum tare is 56% of full scale @13:18:24.35 Pressure.in :default @13:18:24.48 Pressure.hold @13:18:24.74 SamplePump.in :prime @13:18:24.85 SamplePump.seek 10ml,59 seconds @13:18:37.28 Pressure.stop @13:18:37.37 SamplePump.setPosition! 0ml @13:18:37.44 Exhaust.open @13:18:38.88 Bypass.close @13:18:40.48 Pressure.in :default @13:18:40.57 Pressure.hold @13:18:40.90 SamplePump.in :prime @13:18:41.00 SamplePump.seek 100ml,5:16 @13:18:44.13 SamplePump.stop @13:18:44.22 Intake.close @13:18:44.31 Exhaust.close @13:18:45.19 Pressure.stop @13:18:45.52 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 7.2psi vacuum after 2.3ml @13:18:45.54 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.33ml" @13:18:48.41 Gate.power :sampler,:OFF @13:19:05.35 Cmd::Error in n -- Not Filtering @13:19:05.42 UserAbort in n -- User Abort!! @13:19:12.40 -> slots 33=>[:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @13:19:20.07 -> fire :archiveNull_bac @13:19:20.12 Selecting Cartridge 33 @13:19:20.89 Preparing to filter cartridge 33 @13:19:21.17 Gate.power :sampler,:ON @13:19:21.31 Priming sample loop w/100ml, bypass w/10ml @13:19:21.32 SamplePump.setPosition! 0ml @13:19:21.41 Bypass.open @13:19:22.92 Intake.open @13:19:23.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:19:26.28 Vacuum tare is 56% of full scale @13:19:27.04 Pressure.in :default @13:19:27.13 Pressure.hold @13:19:27.41 SamplePump.in :prime @13:19:27.50 SamplePump.seek 10ml,59 seconds @13:19:37.66 SamplePump.stop @13:19:37.75 Intake.close @13:19:37.80 Bypass.close @13:19:38.63 Pressure.stop @13:19:38.93 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 0.5psi vacuum after 8.1ml @13:19:38.95 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 8.11ml" @13:19:41.82 Gate.power :sampler,:OFF @13:21:26.55 Cmd::Error in n -- Not Filtering @13:21:26.62 UserAbort in n -- User Abort!! @13:22:15.27 -> Sampler::PumpPrimeDelay=-5.minutes @13:22:15.30 RuntimeError in n -- cannot redefine constant PumpPrimeDelay @13:22:38.96 -> PumpPrimeDelay=-5.minutes -5:00 @13:23:10.48 -> PumpPrimeDelay=-1.minutes @13:23:10.51 RuntimeError in n -- cannot redefine constant PumpPrimeDelay @13:23:29.44 -> PumpPrimeDelay=-1.minutes @13:23:29.47 RuntimeError in n -- cannot redefine constant PumpPrimeDelay @13:23:47.52 -> Sampler::PumpPrimeDelay 5:00 @13:24:07.44 -> slots 33=> [:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @13:24:14.50 -> fire :archiveNull_bac @13:24:14.54 Selecting Cartridge 33 @13:24:15.32 Preparing to filter cartridge 33 @13:24:15.59 Gate.power :sampler,:ON @13:24:15.72 Priming sample loop w/100ml, bypass w/10ml @13:24:15.74 SamplePump.setPosition! 0ml @13:24:15.82 Bypass.open @13:24:17.34 Intake.open @13:24:18.14 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:24:20.72 Vacuum tare is 56% of full scale @13:24:20.92 Pressure.in :default @13:24:21.01 Pressure.hold @13:24:21.30 SamplePump.in :prime @13:24:21.40 SamplePump.seek 10ml,59 seconds @13:24:33.82 Pressure.stop @13:24:33.90 SamplePump.setPosition! 0ml @13:24:33.98 Exhaust.open @13:24:35.47 Bypass.close @13:24:37.06 Pressure.hold @13:24:37.42 SamplePump.in :prime @13:24:37.53 SamplePump.seek 100ml,5:16 @13:24:40.65 SamplePump.stop @13:24:40.74 Intake.close @13:24:40.79 Exhaust.close @13:24:42.22 Pressure.stop @13:24:42.68 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 6.6psi vacuum after 2.3ml @13:24:42.70 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.34ml" @13:24:45.56 Gate.power :sampler,:OFF @13:24:53.48 Cmd::Error in n -- Not Filtering @13:24:53.54 UserAbort in n -- User Abort!! @13:25:09.32 -> Power.sampler :ON @13:25:09.35 Gate.power :sampler,:ON true @13:27:03.04 -> Power.sampler :OFF @13:27:03.06 Gate.power :sampler,:OFF false @13:27:52.36 -> PB.open;Exhaust.open;Intake.open @13:27:52.42 Bypass.open @13:27:53.22 Exhaust.open @13:27:53.98 Intake.open Intake is opened @13:28:03.35 -> PB.close;Exhaust.close;Intake.close @13:28:03.38 Bypass.close @13:28:04.11 Exhaust.close @13:28:04.84 Intake.close Intake is closed @13:28:23.03 -> Power.sampler :ON @13:28:23.05 Gate.power :sampler,:ON true @13:30:08.41 -> Power.sampler :OFF @13:30:08.44 Gate.power :sampler,:OFF false @13:31:38.16 -> PB.open;Exhaust.open;Intake.open @13:31:38.18 Bypass.open @13:31:38.92 Exhaust.open @13:31:39.68 Intake.open Intake is opened @13:31:42.55 -> Leak.psia 14.1733695527087 @13:33:51.37 -> Power.sampler :ON @13:33:51.40 Gate.power :sampler,:ON true @13:34:13.59 -> Power.sampler :OFF @13:34:13.63 Gate.power :sampler,:OFF false @13:34:35.05 -> Power.sampler :ON @13:34:35.07 Gate.power :sampler,:ON true @13:44:03.44 -> Power.sampler :OFF @13:44:03.46 Gate.power :sampler,:OFF false @13:44:09.36 -> ESP.shutdown @13:44:09.48 Intake.close @13:44:09.53 Exhaust.close @13:44:09.64 Bypass.close @13:44:10.52 Gate.power :motor,:OFF @13:44:10.54 Gate.power :core,:OFF @13:44:10.57 Gate.power :analytic1,:OFF @13:44:10.61 Gate.power :analytic2,:OFF @13:44:10.65 Gate.power :main,:OFF [:motor,:core,:analytic1,:analytic2,:main] @13:54:30.44 -> MainThread.exit # @13:54:30.54 ThreadError in n -- killed thread @13:54:30.62 Session Ended @13:54:30.65 Thread::Aborted in FILTERING -- "Aborted by n" @13:54:30.69 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.34ml" @13:54:30.71 ThreadError in FILTERING -- killed thread @13:54:30.73 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.34ml" @13:57:04.85PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @13:57:06.91 >BREAK< ESP3 Gateway boot v1.43 for core bus @13:57:06.97 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @13:57:10.59 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @13:57:11.21 Sea pressure (Abs) tare is 6% of full scale @13:57:13.71 Polling Can every 2:00 @13:57:13.77 Waiting for espclients... @13:57:14.68 Session Begun @13:57:32.34 -> slot 33=>[:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @13:57:38.55 -> fire :archiveNull_bac @13:57:38.62 Gate.power :sampler,:OFF,limit to 3500mA @13:57:38.65 Gate.power :main,:ON,limit to 5000mA @13:57:38.68 Gate.power :analytic1,:ON,limit to 5000mA @13:57:38.72 Gate.power :analytic2,:ON,limit to 5000mA @13:57:39.30 Powering core bus @13:57:42.10 Archive.configure :default @13:57:42.23 Air.configure :default @13:57:42.33 Lysis1.configure :default @13:57:42.46 Lysis2.configure :default @13:57:42.85 Plunge.configure :default @13:57:42.97 SamplePump.configure :default @13:57:43.10 Toroid.configure :default @13:57:43.23 Carousel.configure :default @13:57:43.35 Pressure.configure :default @13:57:43.47 ExtraPump.configure :default @13:57:43.57 PinPort.configure :default @13:57:43.68 All elf controllers running firmware version 4.84 @13:57:43.73 Gate.power :motor,:ON,limit to 5000mA @13:57:46.08 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:57:46.11 AnalyticModule::Unknown in n -- SPR @13:57:46.47 Archive.home.jog 0 @13:57:47.24 Carousel.home.jog 0 @13:57:47.31 Plunge.home.jog 0 @13:57:47.35 Lysis1.home.jog 0 @13:57:47.38 Air.home.jog 0 @13:57:47.42 Toroid.home.jog 0 @13:57:47.46 Lysis2.home.jog 0 @13:57:47.50 PinPort.home.jog 0 @13:57:48.26 Archive.in :homing @13:57:48.70 Archive.jog 1379 @13:57:48.78 PinPort.in :homing @13:57:48.82 Plunge.in :homing @13:57:48.86 Lysis1.in :homing @13:57:48.94 Lysis2.in :homing @13:57:49.04 Air.in :homing @13:57:49.11 Toroid.in :homing @13:57:49.44 Plunge.jog 1556 @13:57:49.53 Lysis1.jog 2135 @13:57:49.59 PinPort.jog -110 @13:57:49.63 Lysis2.jog 169 @13:57:49.67 Air.jog 1960 @13:57:49.71 Toroid.jog 601 @13:57:50.12 PinPort.setRawPosition! 0 @13:57:50.20 PinPort.seek :home @13:57:50.32 PinPort.in :default @13:57:50.48 Lysis2.setRawPosition! 50 @13:57:50.51 Archive.setRawPosition! 50 @13:57:50.64 Lysis2.seek :home @13:57:50.68 Archive.seek :home @13:57:51.00 Plunge.setRawPosition! 50 @13:57:51.09 Plunge.seek :home @13:57:51.23 Archive.in :default @13:57:51.26 Lysis2.in :default @13:57:51.49 Air.setRawPosition! 50 @13:57:51.54 Plunge.in :default @13:57:51.58 Lysis1.setRawPosition! 50 @13:57:51.77 Air.seek :home @13:57:51.83 Lysis1.seek :home @13:57:52.06 Toroid.setRawPosition! 0 @13:57:52.13 Toroid.seek :home @13:57:52.30 Lysis1.in :default @13:57:52.35 Air.in :default @13:57:52.43 Toroid.in :default @13:57:52.90 Toroid.seek :clear @13:57:54.56 Carousel.in :default @13:57:55.20 Carousel.home.seek 33 @13:57:55.51 Selecting Cartridge 33 @13:57:55.86 Carousel.seek 33 @13:57:56.16 Lysis1.in :fast @13:57:56.26 Lysis1.seek :locked @13:57:58.93 Plunge.seek :locked @13:57:59.13 Air.in :fast @13:57:59.23 Air.seek :locked @13:58:00.84 Carousel.reconfigure :locked @13:58:01.06 Powering cartridge bus @13:58:03.74 Gate.power :cartridge,:OFF @13:58:04.31 Mounted archive Cartridge @13:58:04.72 Preparing to filter cartridge 33 @13:58:04.99 Gate.power :sampler,:ON @13:58:05.12 Priming sample loop w/150ml, bypass w/50ml @13:58:05.13 SamplePump.setPosition! 0ml @13:58:05.22 Bypass.open @13:58:06.78 Intake.open @13:58:07.59 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:58:10.18 Vacuum tare is 56% of full scale @13:58:10.38 Pressure.in :default @13:58:10.49 Pressure.hold @13:58:10.75 Waiting for external sampler to finish priming... @13:58:10.77 delayUntil "14:01:35.01" @14:01:35.07 SamplePump.in :prime @14:01:35.17 SamplePump.seek 50ml,2:53 @14:02:36.18 Pressure.stop @14:02:36.26 SamplePump.setPosition! 0ml @14:02:36.34 Exhaust.open @14:02:37.84 Bypass.close @14:02:39.41 Pressure.in :default @14:02:39.50 Pressure.hold @14:02:39.85 SamplePump.in :prime @14:02:39.96 SamplePump.seek 150ml,7:39 @14:02:43.58 SamplePump.stop @14:02:43.67 Intake.close @14:02:43.71 Exhaust.close @14:02:44.62 Pressure.stop @14:02:44.92 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 18.1psi vacuum after 2.3ml @14:02:44.94 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.33ml" @14:02:47.81 Gate.power :sampler,:OFF @14:04:50.15 Cmd::Error in n -- Not Filtering @14:04:50.23 UserAbort in n -- User Abort!! @14:04:57.93 -> slot 33=>[:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @14:05:11.35 -> fire :archiveNull_bac @14:05:11.39 Selecting Cartridge 33 @14:05:12.14 Preparing to filter cartridge 33 @14:05:12.46 Gate.power :sampler,:ON @14:05:12.58 Priming sample loop w/150ml, bypass w/50ml @14:05:12.60 SamplePump.setPosition! 0ml @14:05:12.68 Bypass.open @14:05:14.23 Intake.open @14:05:14.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:05:17.58 Vacuum tare is 55% of full scale @14:05:17.78 Pressure.in :default @14:05:17.88 Pressure.hold @14:05:18.14 Waiting for external sampler to finish priming... @14:05:18.16 delayUntil "14:08:42.48" @14:08:42.53 SamplePump.in :prime @14:08:42.63 SamplePump.seek 50ml,2:53 @14:09:43.63 Pressure.stop @14:09:43.71 SamplePump.setPosition! 0ml @14:09:43.78 Exhaust.open @14:09:45.22 Bypass.close @14:09:46.79 Pressure.in :default @14:09:46.89 Pressure.hold @14:09:47.23 SamplePump.in :prime @14:09:47.34 SamplePump.seek 150ml,7:39 @14:09:50.47 SamplePump.stop @14:09:50.56 Intake.close @14:09:50.67 Exhaust.close @14:09:51.52 Pressure.stop @14:09:51.83 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 12.4psi vacuum after 2.3ml @14:09:51.86 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.33ml" @14:09:54.71 Gate.power :sampler,:OFF @14:10:14.19 Cmd::Error in n -- Not Filtering @14:10:14.27 UserAbort in n -- User Abort!! @14:10:38.87 -> Leak.psia 14.3325217997439 @14:10:52.92 -> slot 33=> :archiveNull_bac {33 => { Type: :archiveNull_bac, State: :leakyToroid}} @14:11:04.54 -> fire :archiveNull_bac @14:11:05.09 Cartridge::None in n -- No dry archiveNull_bac cartridges remain @14:11:25.82 -> slot 33=> [:dry,:archiveNull_bac] {33 => { Type: :archiveNull_bac, State: :dry}} @14:11:30.71 -> fire :archiveNull_bac @14:11:30.75 Selecting Cartridge 33 @14:11:31.51 Preparing to filter cartridge 33 @14:11:31.81 Gate.power :sampler,:ON @14:11:31.93 Priming sample loop w/150ml, bypass w/50ml @14:11:31.95 SamplePump.setPosition! 0ml @14:11:32.03 Bypass.open @14:11:33.56 Intake.open @14:11:34.31 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:11:36.91 Vacuum tare is 55% of full scale @14:11:37.11 Pressure.in :default @14:11:37.20 Pressure.hold @14:11:37.46 Waiting for external sampler to finish priming... @14:11:37.47 delayUntil "14:15:01.83" @14:15:01.89 SamplePump.in :prime @14:15:01.99 SamplePump.seek 50ml,2:53 @14:16:03.00 Pressure.stop @14:16:03.08 SamplePump.setPosition! 0ml @14:16:03.15 Exhaust.open @14:16:04.59 Bypass.close @14:16:06.17 Pressure.in :default @14:16:06.28 Pressure.hold @14:16:06.67 SamplePump.in :prime @14:16:06.77 SamplePump.seek 150ml,7:39 @14:19:09.14 Pressure.stop @14:19:09.24 Bypass.open @14:19:10.00 Intake.close @14:19:10.79 Toroid.in :hold @14:19:10.88 Toroid.seek :crack @14:19:12.45 Toroid.seek :cartridge @14:19:13.60 Toroid.in :default @14:19:13.70 Toroid.seek :clear @14:19:15.74 Pressurizing cartridge to 26psi above 13.4psia @14:19:15.76 SamplePump.setPosition! 0ml @14:19:15.85 Intake.open @14:19:16.53 Bypass.close @14:19:17.25 Exhaust.close @14:19:18.12 Pressure.in :default @14:19:18.77 Pressure.hold @14:19:19.03 SamplePump.in :charge @14:19:19.14 SamplePump.seek 2.3ml,42 seconds @14:19:28.69 Pressure.stop @14:19:28.78 Intake.close @14:19:29.56 Pressurized cartridge with 0.408ml to 26.5psi @14:19:29.58 Isolated @34.0psia (20.6psi) @14:19:29.61 Verifying cartridge holds at least 0.65..0.85 of 34.0psia @14:19:29.67 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:19:34.36 Sampling 1000.0ml @14:19:34.41 SamplePump.setPosition! 0ml @14:19:34.51 Exhaust.open @14:19:35.28 Intake.open @14:19:36.13 Pressure.in :default @14:19:36.23 Pressure.hold @14:19:36.43 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:19:36.65 SamplePump.in :sample @14:19:36.75 SamplePump.seek 1000ml,1:23:51 @14:19:39.77 Cartridge 33 filtering 1/1000ml at 0.59ml/s with 19.4psi vacuum @14:20:39.78 Cartridge 33 filtering 36/1000ml at 0.53ml/s with 28.3psi vacuum @14:21:39.79 Cartridge 33 filtering 69/1000ml at 0.53ml/s with 16.0psi vacuum @14:22:39.79 Cartridge 33 filtering 102/1000ml at 0.58ml/s with 16.5psi vacuum 2.1m deep (17.8psia) @14:23:39.78 Cartridge 33 filtering 136/1000ml at 0.55ml/s with 20.4psi vacuum @14:24:39.78 Cartridge 33 filtering 169/1000ml at 0.51ml/s with 13.5psi vacuum @14:25:39.79 Cartridge 33 filtering 202/1000ml at 0.55ml/s with 22.6psi vacuum @14:26:39.78 Cartridge 33 filtering 235/1000ml at 0.59ml/s with 25.7psi vacuum @14:27:39.78 Cartridge 33 filtering 269/1000ml at 0.57ml/s with 22.0psi vacuum @14:28:39.79 Cartridge 33 filtering 302/1000ml at 0.57ml/s with 23.7psi vacuum @14:29:39.78 Cartridge 33 filtering 335/1000ml at 0.50ml/s with 28.4psi vacuum @14:30:09.33 SamplePump.stop @14:30:09.50 Intake.close @14:30:09.57 Exhaust.close @14:30:09.89 Toroid.in :hold @14:30:09.99 Toroid.seek :bypass @14:30:11.67 Toroid.in :default @14:30:11.77 Toroid.seek :clear @14:30:13.42 Pressure.stop @14:30:14.42 Cmd::Paused in FILTERING -- paused by n during Sample Pump (SP) move after sampling 351.7ml @14:30:14.49 UserAbort in n -- User Abort!! @14:30:17.37 Gate.power :sampler,:OFF @14:30:18.38 -> ESP.reready! @14:30:18.46 Archive.in :default @14:30:18.52 Air.in :default @14:30:18.68 Lysis1.in :default @14:30:18.79 Lysis2.in :default @14:30:18.94 Plunge.in :default @14:30:19.04 Archive.home.jog 0 @14:30:19.12 Toroid.in :default @14:30:19.17 Carousel.in :default @14:30:19.25 Air.home.jog 0 @14:30:19.36 PinPort.in :default @14:30:19.42 Lysis1.home.jog 0 @14:30:19.64 Lysis2.home.jog 0 @14:30:19.73 Plunge.home.jog 0 @14:30:20.07 PinPort.home.jog 0 @14:30:20.19 Carousel.home.jog 0 @14:30:20.29 Toroid.home.jog 0 @14:30:21.09 Archive.in :homing @14:30:21.56 Archive.jog 1382 @14:30:21.64 Toroid.in :homing @14:30:21.69 Lysis2.in :homing @14:30:21.75 Air.in :homing @14:30:21.79 Plunge.in :homing @14:30:21.87 Lysis1.in :homing @14:30:21.93 PinPort.in :homing @14:30:22.31 Toroid.jog 601 @14:30:22.40 Lysis2.jog 1392 @14:30:22.47 Air.jog 1959 @14:30:22.53 Plunge.jog 1555 @14:30:22.63 PinPort.jog -110 @14:30:22.67 Lysis1.jog 2130 @14:30:23.15 PinPort.setRawPosition! 0 @14:30:23.23 PinPort.seek :home @14:30:23.36 Archive.setRawPosition! 50 @14:30:23.39 PinPort.in :default @14:30:23.52 Archive.seek :home @14:30:23.85 Archive.in :default @14:30:23.95 Lysis2.setRawPosition! 50 @14:30:24.03 Lysis2.seek :home @14:30:24.15 Plunge.setRawPosition! 50 @14:30:24.23 Plunge.seek :home @14:30:24.38 Air.setRawPosition! 50 @14:30:24.45 Lysis2.in :default @14:30:24.59 Air.seek :home @14:30:24.76 Lysis1.setRawPosition! 50 @14:30:24.79 Toroid.setRawPosition! 0 @14:30:24.83 Plunge.in :default @14:30:24.99 Lysis1.seek :home @14:30:25.07 Toroid.seek :home @14:30:25.28 Air.in :default @14:30:25.31 Toroid.in :default @14:30:25.49 Lysis1.in :default @14:30:25.86 Toroid.seek :clear @14:30:27.50 Carousel.in :default @14:30:27.66 Carousel.home.seek 33 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 351.67ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 33 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @14:30:32.06 -> slots 1..60=>nil {60..1 => nil} @14:36:08.14 -> ESP.shutdown @14:36:08.33 Gate.power :motor,:OFF @14:36:08.35 Gate.power :core,:OFF @14:36:08.42 Gate.power :analytic1,:OFF @14:36:08.46 Gate.power :analytic2,:OFF @14:36:08.49 Gate.power :main,:OFF [:motor,:core,:analytic1,:analytic2,:main] @14:36:11.33 -> exit @14:36:11.35 Session Ended @20:37:03.91PDT04-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @20:37:05.56 >BREAK< ESP3 Gateway boot v1.43 for core bus @20:37:05.59 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @20:37:09.02 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @20:37:09.65 Sea pressure (Abs) tare is 6% of full scale @20:37:12.40 Polling Can every 2:00 @20:37:12.81 Loading /home/esp/esp2/bin/processAll mission @20:37:13.06 Sea pressure (Abs) tare is 6% of full scale @20:37:13.07 Sampling default goal volumes @20:37:13.09 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @20:37:13.26 Gate.power :sampler,:OFF,limit to 3500mA @20:37:13.28 Gate.power :analytic1,:ON,limit to 5000mA @20:37:13.29 Gate.power :analytic2,:ON,limit to 5000mA @20:37:14.14 Archive.configure :default @20:37:14.23 SPR Module 1, FW Rev. B, FPGA Rev. C @20:37:14.54
Air.configure :default @20:37:14.78 Lysis1.configure :default @20:37:15.07 Lysis2.configure :default @20:37:15.25 Session Begun @20:37:15.59
Plunge.configure :default @20:37:15.86 SamplePump.configure :default @20:37:16.05 Toroid.configure :default @20:37:16.24 Carousel.configure :default @20:37:16.42 Pressure.configure :default @20:37:16.60 ExtraPump.configure :default @20:37:16.75 PinPort.configure :default @20:37:16.84 All elf controllers running firmware version 4.84 @20:37:17.19 Archive.in :default @20:37:17.70 Archive.home.jog 0 @20:37:18.01 PinPort.in :default @20:37:18.06 Carousel.in :default @20:37:18.11 Toroid.in :default @20:37:18.18 Lysis2.in :default @20:37:18.22 Air.in :default @20:37:18.27 Lysis1.in :default @20:37:18.40 Plunge.in :default @20:37:18.73 Carousel.home.jog 0 @20:37:18.80 PinPort.home.jog 0 @20:37:18.85 Lysis2.home.jog 0 @20:37:18.89 Toroid.home.jog 0 @20:37:18.92 Air.home.jog 0 @20:37:19.01 Plunge.home.jog 0 @20:37:19.05 Lysis1.home.jog 0 @20:37:19.88 Archive.in :homing @20:37:20.20 Archive.jog 1382 @20:37:20.28 PinPort.in :homing @20:37:20.34 Plunge.in :homing @20:37:20.39 Lysis1.in :homing @20:37:20.44 Toroid.in :homing @20:37:20.50 Lysis2.in :homing @20:37:20.57 Air.in :homing @20:37:20.95 PinPort.jog -110 @20:37:21.00 Toroid.jog 599 @20:37:21.05 Plunge.jog 399 @20:37:21.08 Lysis1.jog 1386 @20:37:21.12 Lysis2.jog 1390 @20:37:21.17 Air.jog 1361 @20:37:22.11 Archive.setRawPosition! 50 @20:37:22.14 PinPort.setRawPosition! 0 @20:37:22.27 Archive.seek :home @20:37:22.31 PinPort.seek :home @20:37:22.52 Plunge.setRawPosition! 50 @20:37:22.55 PinPort.in :default @20:37:22.68 Plunge.seek :home @20:37:22.82 Archive.in :default @20:37:23.01 Plunge.in :default @20:37:23.16 Lysis1.setRawPosition! 50 @20:37:23.20 Air.setRawPosition! 50 @20:37:23.31 Lysis1.seek :home @20:37:23.45 Air.seek :home @20:37:23.59 Lysis2.setRawPosition! 50 @20:37:23.70 Lysis2.seek :home @20:37:23.88 Air.in :default @20:37:23.91 Toroid.setRawPosition! 0 @20:37:23.96 Lysis1.in :default @20:37:24.18 Toroid.seek :home @20:37:24.31 Lysis2.in :default @20:37:24.34 Toroid.in :default @20:37:24.73
Toroid.seek :clear @20:37:26.36 Carousel.in :default @20:37:26.52 Carousel.home.seek 32 @20:37:26.87 Selecting Cartridge 30 @20:37:27.22 Carousel.seek 30 @20:37:27.99 Lysis1.in :fast @20:37:28.08 Lysis1.seek :locked @20:37:30.73 Plunge.seek :locked @20:37:30.92 Air.in :fast @20:37:31.01 Air.seek :locked @20:37:32.61
Carousel.reconfigure :locked @20:37:32.82 Powering cartridge bus @20:37:35.47 Gate.setIR 1750,5000,0,6 @20:37:35.60 Gate.setIR 0,5000,0,6 @20:37:35.70 PuckHeater.configure :default @20:37:35.79 Gate.power :heat,:ON,limit to 5000mA @20:37:36.45 Mounted lysis Cartridge @20:37:36.49 WARNING: Using default puck temperature sensor calibration @20:37:36.52 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:37:38.12 SPR.command "read_sequences" @20:37:38.64 SPR.command "stats_on" @20:37:38.81 SPR.command "start 0 1" @20:37:39.52 SPR.start /var/log/esp/spr23aug0420h.spr.bz2 @20:37:41.49 Waiting for baseline SPR stabilization @20:37:41.51 Handoff.select :waste @20:37:48.25 Syringe tare is 19% of full scale @20:37:48.28 PinPort.in :locking @20:37:48.40 PinPort.seek :locked @20:37:50.96 Archive.in :fast @20:37:51.01 Lysis2.in :fast @20:37:51.12 Lysis1.seek 1000uL @20:37:51.25 Archive.seek :full @20:37:51.45 Lysis2.seek 1000uL @20:37:56.03 delayUntil "20:47:41.03" @20:47:41.05 Lysis2.in :DAmix @20:47:41.20 Lysis1.in :DAmix @20:47:41.77 Lysis2.seek 750uL @20:47:41.84 Lysis1.seek 750uL @20:48:16.96 delay 5 seconds @20:48:21.99 Handoff.select SPR @20:48:22.14 SPR.command "start 2 14" @20:48:22.76 SPR stabilized @20:48:22.94 delay 10 seconds @20:48:32.97 SPR.command "start 2 0" @20:48:36.65 Waiting up to 11:00 for SPR to enter state 1 @20:50:34.75 SPR.command "hold_on" @20:50:36.13 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:50:36.76 Lysis2.seek 450uL @20:50:36.82 Lysis1.seek 450uL @20:51:18.91 delay 15 seconds @20:51:33.94 SPR.command "hold_off" @20:51:36.78 Waiting up to 38:00 for SPR to enter state 7 @21:05:56.95 SPRsummary:19.9RIU,31.8RIU,128RIU,151RIU after 1792s @21:17:27.56 delay 10 seconds @21:17:37.59 SPR.command "start 2 0" @21:17:41.18 Waiting up to 11:00 for SPR to enter state 1 @21:19:38.84 SPR.command "hold_on" @21:19:40.23 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:19:40.79 Lysis2.seek 300uL @21:19:40.85 Lysis1.seek 300uL @21:20:02.00 delay 15 seconds @21:20:17.02 SPR.command "hold_off" @21:20:20.08 Waiting up to 38:00 for SPR to enter state 7 @21:46:10.83 delay 10 seconds @21:46:20.85 SPR.command "start 2 0" @21:46:23.54 Waiting up to 11:00 for SPR to enter state 1 @21:48:21.21 SPR.command "hold_on" @21:48:22.59 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:48:23.27 Lysis2.seek 150uL @21:48:23.35 Lysis1.seek 150uL @21:48:44.50 delay 15 seconds @21:48:59.53 SPR.command "hold_off" @21:49:02.95 Waiting up to 38:00 for SPR to enter state 7 @22:14:54.13 delay 10 seconds @22:15:04.15 SPR.command "start 2 0" @22:15:07.36 Waiting up to 11:00 for SPR to enter state 1 @22:17:05.46 SPR.command "hold_on" @22:17:06.83 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:17:07.57 Lysis2.seek 0uL @22:17:07.60 Lysis1.seek 0uL @22:17:28.75 delay 15 seconds @22:17:43.77 SPR.command "hold_off" @22:17:47.20 Waiting up to 38:00 for SPR to enter state 7 @22:43:38.23 SPR analysis completed @22:43:42.17 Ended log spr23aug0420h.spr.bz2 @22:43:42.19 SPR.command "stats_off" @22:43:42.61 Intake.close @22:43:42.66 Exhaust.close @22:43:42.83 Bypass.close @22:43:42.88 Handoff.select :close @22:43:43.13
Gate.power :heat,:OFF @22:43:43.15 Gate.power :cartridge,:OFF @22:43:43.28 PinPort.in :unlocking @22:43:43.36 PinPort.seek :clear @22:43:45.35 Carousel.in :default @22:43:45.54 Carousel.home.seek 30 @22:43:45.90 Plunge.seek :locked @22:43:46.10 Archive.in :unlocking @22:43:46.28 Archive.seek :clear @22:43:46.84 Air.in :unlocking @22:43:47.05 Air.seek :clear @22:43:47.16 Lysis1.in :unlocking @22:43:47.35 Lysis1.seek :clear @22:43:47.53 Lysis2.in :unlocking @22:43:47.64 Lysis2.seek :clear @22:43:52.27
Gate.power :motor,:OFF @22:43:52.29 Gate.power :core,:OFF @22:43:52.34 Gate.power :analytic1,:OFF @22:43:52.37 Gate.power :analytic2,:OFF @22:43:52.40 Gate.power :main,:OFF @22:43:52.43 Safely stopped and ready to power off @22:43:52.96 Gate.power :sampler,:OFF,limit to 3500mA @22:43:56.14 Gate.power :main,:ON,limit to 5000mA @22:43:56.16 Gate.power :analytic1,:ON,limit to 5000mA @22:43:56.21 Gate.power :analytic2,:ON,limit to 5000mA @22:43:56.30 Powering core bus @22:43:58.67 SPR Module 1, FW Rev. B, FPGA Rev. C @22:43:59.11
Archive.configure :default @22:43:59.23 Air.configure :default @22:43:59.32 Lysis1.configure :default @22:43:59.45 Lysis2.configure :default @22:43:59.56 Plunge.configure :default @22:43:59.68 SamplePump.configure :default @22:43:59.79 Toroid.configure :default @22:43:59.91 Carousel.configure :default @22:44:00.03 Pressure.configure :default @22:44:00.14 ExtraPump.configure :default @22:44:00.23 PinPort.configure :default @22:44:00.31 All elf controllers running firmware version 4.84 @22:44:00.34 Gate.power :motor,:ON,limit to 5000mA @22:44:00.59 Archive.home.jog 0 @22:44:01.43 Carousel.home.jog 0 @22:44:01.48 Toroid.home.jog 0 @22:44:01.54 Lysis2.home.jog 0 @22:44:01.58 Lysis1.home.jog 0 @22:44:01.63 Air.home.jog 0 @22:44:01.68 PinPort.home.jog 0 @22:44:01.73 Plunge.home.jog 0 @22:44:02.69 Archive.in :homing @22:44:03.00 Archive.jog 1380 @22:44:03.08 Toroid.in :homing @22:44:03.13 Lysis2.in :homing @22:44:03.17 Air.in :homing @22:44:03.23 PinPort.in :homing @22:44:03.30 Lysis1.in :homing @22:44:03.36 Plunge.in :homing @22:44:03.66 Toroid.jog 604 @22:44:03.75 Lysis2.jog 1390 @22:44:03.93 Plunge.jog 1550 @22:44:04.00 Lysis1.jog 1385 @22:44:04.05 PinPort.jog -108 @22:44:04.09 Air.jog 1359 @22:44:04.51 PinPort.setRawPosition! 0 @22:44:04.60 PinPort.seek :home @22:44:04.74 Archive.setRawPosition! 50 @22:44:04.77 PinPort.in :default @22:44:04.89 Archive.seek :home @22:44:05.67 Lysis2.setRawPosition! 50 @22:44:05.72 Lysis1.setRawPosition! 50 @22:44:05.76 Plunge.setRawPosition! 50 @22:44:05.79 Air.setRawPosition! 50 @22:44:05.97 Lysis2.seek :home @22:44:06.08 Plunge.seek :home @22:44:06.14 Air.seek :home @22:44:06.19 Lysis1.seek :home @22:44:06.55 Toroid.setRawPosition! 0 @22:44:06.57 Archive.in :default @22:44:06.72 Toroid.seek :home @22:44:06.91 Toroid.in :default @22:44:06.96 Plunge.in :default @22:44:07.00 Lysis1.in :default @22:44:07.05 Lysis2.in :default @22:44:07.11 Air.in :default @22:44:07.71
Toroid.seek :clear @22:44:09.36 Carousel.in :default @22:44:09.56 Carousel.home.seek 30 @22:44:09.85 Selecting Cartridge 29 @22:44:10.18 Carousel.seek 29 @22:44:10.75 Lysis1.in :fast @22:44:10.84 Lysis1.seek :locked @22:44:13.53 Plunge.seek :locked @22:44:13.72 Air.in :fast @22:44:13.81 Air.seek :locked @22:44:15.43
Carousel.reconfigure :locked @22:44:15.62 Powering cartridge bus @22:44:18.27 Gate.setIR 1750,5000,0,6 @22:44:18.40 Gate.setIR 0,5000,0,6 @22:44:18.49 PuckHeater.configure :default @22:44:18.58 Gate.power :heat,:ON,limit to 5000mA @22:44:19.19 Mounted lysis Cartridge @22:44:19.23 WARNING: Using default puck temperature sensor calibration @22:44:19.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:44:20.88 SPR.command "read_sequences" @22:44:21.21 SPR.command "stats_on" @22:44:21.40 SPR.command "start 0 1" @22:44:22.09 SPR.start /var/log/esp/spr23aug0422h.spr.bz2 @22:44:24.07 Waiting for baseline SPR stabilization @22:44:24.09 Handoff.select :waste @22:44:30.99 Syringe tare is 19% of full scale @22:44:31.02 PinPort.in :locking @22:44:31.16 PinPort.seek :locked @22:44:33.75 Archive.in :fast @22:44:33.88 Lysis2.in :fast @22:44:33.95 Lysis1.seek 1000uL @22:44:34.00 Archive.seek :full @22:44:34.23 Lysis2.seek 1000uL @22:44:38.80 delayUntil "22:54:23.65" @22:54:23.66 Lysis2.in :DAmix @22:54:23.82 Lysis1.in :DAmix @22:54:24.36 Lysis2.seek 750uL @22:54:24.39 Lysis1.seek 750uL @22:54:59.56 delay 5 seconds @22:55:04.59 Handoff.select SPR @22:55:04.74 SPR.command "start 2 14" @22:55:05.34 SPR stabilized @22:55:05.57 delay 10 seconds @22:55:15.59 SPR.command "start 2 0" @22:55:18.71 Waiting up to 11:00 for SPR to enter state 1 @22:57:16.38 SPR.command "hold_on" @22:57:18.29 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:57:18.92 Lysis2.seek 450uL @22:57:19.01 Lysis1.seek 450uL @22:58:01.08 delay 15 seconds @22:58:16.10 SPR.command "hold_off" @22:58:19.36 Waiting up to 38:00 for SPR to enter state 7 @23:12:38.59 SPRsummary:21.7RIU,31.7RIU,119RIU,158RIU after 1791s @23:24:09.64 delay 10 seconds @23:24:19.66 SPR.command "start 2 0" @23:24:22.82 Waiting up to 11:00 for SPR to enter state 1 @23:26:20.49 SPR.command "hold_on" @23:26:21.87 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:26:22.46 Lysis2.seek 300uL @23:26:22.54 Lysis1.seek 300uL @23:26:43.67 delay 15 seconds @23:26:58.69 SPR.command "hold_off" @23:27:01.72 Waiting up to 38:00 for SPR to enter state 7 @23:52:52.48 delay 10 seconds @23:53:02.50 SPR.command "start 2 0" @23:53:05.18 Waiting up to 11:00 for SPR to enter state 1 @23:55:02.84 SPR.command "hold_on" @23:55:04.23 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:55:04.94 Lysis2.seek 150uL @23:55:05.04 Lysis1.seek 150uL @23:55:26.15 delay 15 seconds @23:55:41.17 SPR.command "hold_off" @23:55:44.60 Waiting up to 38:00 for SPR to enter state 7 @00:21:35.79PDT05-Aug-23 delay 10 seconds @00:21:45.81 SPR.command "start 2 0" @00:21:48.49 Waiting up to 11:00 for SPR to enter state 1 @00:23:46.15 SPR.command "hold_on" @00:23:48.06 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:23:48.75 Lysis2.seek 0uL @00:23:48.79 Lysis1.seek 0uL @00:24:09.92 delay 15 seconds @00:24:24.95 SPR.command "hold_off" @00:24:28.85 Waiting up to 38:00 for SPR to enter state 7 @00:50:18.94 SPR analysis completed @00:50:22.89 Ended log spr23aug0422h.spr.bz2 @00:50:22.91 SPR.command "stats_off" @00:50:23.33 Handoff.select :close @00:50:23.53
Gate.power :heat,:OFF @00:50:23.55 Gate.power :cartridge,:OFF @00:50:23.68 PinPort.in :unlocking @00:50:23.76 PinPort.seek :clear @00:50:25.71 Carousel.in :default @00:50:25.86 Carousel.home.seek 29 @00:50:26.23 Plunge.seek :locked @00:50:26.43 Archive.in :unlocking @00:50:26.63 Archive.seek :clear @00:50:26.74 Air.in :unlocking @00:50:27.42 Air.seek :clear @00:50:27.48 Lysis1.in :unlocking @00:50:27.74 Lysis1.seek :clear @00:50:27.80 Lysis2.in :unlocking @00:50:27.97 Lysis2.seek :clear @00:50:32.60
Gate.power :motor,:OFF @00:50:32.62 Gate.power :core,:OFF @00:50:32.66 Gate.power :analytic1,:OFF @00:50:32.69 Gate.power :analytic2,:OFF @00:50:32.73 Gate.power :main,:OFF @00:50:32.75 Safely stopped and ready to power off @00:50:32.97 Gate.power :sampler,:OFF,limit to 3500mA @00:50:36.47 Gate.power :main,:ON,limit to 5000mA @00:50:36.49 Gate.power :analytic1,:ON,limit to 5000mA @00:50:36.53 Gate.power :analytic2,:ON,limit to 5000mA @00:50:36.63 Powering core bus @00:50:38.98 SPR Module 1, FW Rev. B, FPGA Rev. C @00:50:39.45
Archive.configure :default @00:50:39.57 Air.configure :default @00:50:39.66 Lysis1.configure :default @00:50:39.75 Lysis2.configure :default @00:50:39.87 Plunge.configure :default @00:50:39.99 SamplePump.configure :default @00:50:40.11 Toroid.configure :default @00:50:40.23 Carousel.configure :default @00:50:40.34 Pressure.configure :default @00:50:40.46 ExtraPump.configure :default @00:50:40.55 PinPort.configure :default @00:50:40.64 All elf controllers running firmware version 4.84 @00:50:40.66 Gate.power :motor,:ON,limit to 5000mA @00:50:40.99 Archive.home.jog 0 @00:50:41.78 PinPort.home.jog 0 @00:50:41.84 Toroid.home.jog 0 @00:50:41.90 Plunge.home.jog 0 @00:50:41.93 Lysis1.home.jog 0 @00:50:41.98 Lysis2.home.jog 0 @00:50:42.03 Air.home.jog 0 @00:50:42.07 Carousel.home.jog 0 @00:50:43.06 Archive.in :homing @00:50:43.30 Archive.jog 1382 @00:50:43.39 PinPort.in :homing @00:50:43.46 Plunge.in :homing @00:50:43.50 Lysis1.in :homing @00:50:43.56 Toroid.in :homing @00:50:43.62 Air.in :homing @00:50:43.69 Lysis2.in :homing @00:50:44.07 PinPort.jog -110 @00:50:44.14 Plunge.jog 1559 @00:50:44.20 Air.jog 1364 @00:50:44.24 Toroid.jog 597 @00:50:44.29 Lysis2.jog 1392 @00:50:44.39 Lysis1.jog 1386 @00:50:44.77 PinPort.setRawPosition! 0 @00:50:44.85 PinPort.seek :home @00:50:44.99 PinPort.in :default @00:50:45.01 Archive.setRawPosition! 50 @00:50:45.17 Archive.seek :home @00:50:46.03 Plunge.setRawPosition! 50 @00:50:46.09 Lysis2.setRawPosition! 50 @00:50:46.12 Air.setRawPosition! 50 @00:50:46.16 Lysis1.setRawPosition! 50 @00:50:46.20 Archive.in :default @00:50:46.43 Plunge.seek :home @00:50:46.50 Lysis2.seek :home @00:50:46.62 Air.seek :home @00:50:46.69 Lysis1.seek :home @00:50:47.01 Plunge.in :default @00:50:47.04 Toroid.setRawPosition! 0 @00:50:47.20 Toroid.seek :home @00:50:47.40 Lysis2.in :default @00:50:47.45 Lysis1.in :default @00:50:47.50 Toroid.in :default @00:50:47.59 Air.in :default @00:50:48.06
Toroid.seek :clear @00:50:49.70 Carousel.in :default @00:50:49.87 Carousel.home.seek 29 @00:50:50.16 Selecting Cartridge 28 @00:50:50.51 Carousel.seek 28 @00:50:51.07 Lysis1.in :fast @00:50:51.16 Lysis1.seek :locked @00:50:53.81 Plunge.seek :locked @00:50:54.01 Air.in :fast @00:50:54.10 Air.seek :locked @00:50:55.70
Carousel.reconfigure :locked @00:50:55.89 Powering cartridge bus @00:50:58.54 Gate.setIR 1750,5000,0,6 @00:50:58.67 Gate.setIR 0,5000,0,6 @00:50:58.75 PuckHeater.configure :default @00:50:58.84 Gate.power :heat,:ON,limit to 5000mA @00:50:59.49 Mounted lysis Cartridge @00:50:59.52 WARNING: Using default puck temperature sensor calibration @00:50:59.56 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:51:01.15 SPR.command "read_sequences" @00:51:01.54 SPR.command "stats_on" @00:51:01.71 SPR.command "start 0 1" @00:51:02.42 SPR.start /var/log/esp/spr23aug0500h.spr.bz2 @00:51:04.39 Waiting for baseline SPR stabilization @00:51:04.41 Handoff.select :waste @00:51:11.26 Syringe tare is 19% of full scale @00:51:11.29 PinPort.in :locking @00:51:11.42 PinPort.seek :locked @00:51:14.00 Archive.in :fast @00:51:14.13 Lysis2.in :fast @00:51:14.19 Lysis1.seek 1000uL @00:51:14.23 Archive.seek :full @00:51:14.47 Lysis2.seek 1000uL @00:51:19.05 delayUntil "01:01:03.96" @01:01:03.98 Lysis2.in :DAmix @01:01:04.14 Lysis1.in :DAmix @01:01:04.68 Lysis2.seek 750uL @01:01:04.71 Lysis1.seek 750uL @01:01:39.85 delay 5 seconds @01:01:44.87 Handoff.select SPR @01:01:45.03 SPR.command "start 2 14" @01:01:45.66 SPR stabilized @01:01:45.86 delay 10 seconds @01:01:55.88 SPR.command "start 2 0" @01:01:59.03 Waiting up to 11:00 for SPR to enter state 1 @01:03:56.70 SPR.command "hold_on" @01:03:58.09 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:03:58.76 Lysis2.seek 450uL @01:03:58.79 Lysis1.seek 450uL @01:04:40.87 delay 15 seconds @01:04:55.89 SPR.command "hold_off" @01:04:58.74 Waiting up to 38:00 for SPR to enter state 7 @01:19:18.92 SPRsummary:19.3RIU,31.5RIU,113RIU,157RIU after 1791s @01:30:49.54 delay 10 seconds @01:30:59.56 SPR.command "start 2 0" @01:31:02.20 Waiting up to 11:00 for SPR to enter state 1 @01:32:59.88 SPR.command "hold_on" @01:33:01.26 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:33:01.91 Lysis2.seek 300uL @01:33:02.01 Lysis1.seek 300uL @01:33:23.11 delay 15 seconds @01:33:38.13 SPR.command "hold_off" @01:33:41.10 Waiting up to 38:00 for SPR to enter state 7 @01:59:31.86 delay 10 seconds @01:59:41.89 SPR.command "start 2 0" @01:59:44.57 Waiting up to 11:00 for SPR to enter state 1 @02:01:42.24 SPR.command "hold_on" @02:01:44.14 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:01:44.82 Lysis2.seek 150uL @02:01:44.91 Lysis1.seek 150uL @02:02:06.01 delay 15 seconds @02:02:21.04 SPR.command "hold_off" @02:02:24.41 Waiting up to 38:00 for SPR to enter state 7 @02:28:14.23 delay 10 seconds @02:28:24.25 SPR.command "start 2 0" @02:28:27.48 Waiting up to 11:00 for SPR to enter state 1 @02:30:25.55 SPR.command "hold_on" @02:30:26.93 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:30:27.67 Lysis2.seek 0uL @02:30:27.73 Lysis1.seek 0uL @02:30:48.86 delay 15 seconds @02:31:03.88 SPR.command "hold_off" @02:31:06.78 Waiting up to 38:00 for SPR to enter state 7 @02:56:57.41 SPR analysis completed @02:57:01.48 Ended log spr23aug0500h.spr.bz2 @02:57:01.50 SPR.command "stats_off" @02:57:02.29 Handoff.select :close @02:57:02.46
Gate.power :heat,:OFF @02:57:02.48 Gate.power :cartridge,:OFF @02:57:02.60 PinPort.in :unlocking @02:57:02.69 PinPort.seek :clear @02:57:04.64 Carousel.in :default @02:57:04.80 Carousel.home.seek 28 @02:57:05.17 Plunge.seek :locked @02:57:05.87 Archive.in :unlocking @02:57:06.04 Archive.seek :clear @02:57:06.18 Air.in :unlocking @02:57:06.38 Air.seek :clear @02:57:06.54 Lysis1.in :unlocking @02:57:06.70 Lysis1.seek :clear @02:57:06.80 Lysis2.in :unlocking @02:57:06.92 Lysis2.seek :clear @02:57:11.59
Gate.power :motor,:OFF @02:57:11.61 Gate.power :core,:OFF @02:57:11.66 Gate.power :analytic1,:OFF @02:57:11.69 Gate.power :analytic2,:OFF @02:57:11.72 Gate.power :main,:OFF @02:57:11.75 Safely stopped and ready to power off @02:57:11.97 Gate.power :sampler,:OFF,limit to 3500mA @02:57:15.47 Gate.power :main,:ON,limit to 5000mA @02:57:15.48 Gate.power :analytic1,:ON,limit to 5000mA @02:57:15.53 Gate.power :analytic2,:ON,limit to 5000mA @02:57:15.63 Powering core bus @02:57:17.99 SPR Module 1, FW Rev. B, FPGA Rev. C @02:57:18.41
Archive.configure :default @02:57:18.53 Air.configure :default @02:57:18.62 Lysis1.configure :default @02:57:18.72 Lysis2.configure :default @02:57:18.84 Plunge.configure :default @02:57:18.96 SamplePump.configure :default @02:57:19.08 Toroid.configure :default @02:57:19.20 Carousel.configure :default @02:57:19.32 Pressure.configure :default @02:57:19.46 ExtraPump.configure :default @02:57:19.55 PinPort.configure :default @02:57:19.64 All elf controllers running firmware version 4.84 @02:57:19.67 Gate.power :motor,:ON,limit to 5000mA @02:57:20.02 Archive.home.jog 0 @02:57:20.73 Carousel.home.jog 0 @02:57:20.78 Toroid.home.jog 0 @02:57:20.84 Plunge.home.jog 0 @02:57:20.89 Lysis1.home.jog 0 @02:57:20.92 Air.home.jog 0 @02:57:20.99 Lysis2.home.jog 0 @02:57:21.02 PinPort.home.jog 0 @02:57:21.86 Archive.in :homing @02:57:22.35 Archive.jog 1382 @02:57:22.43 Toroid.in :homing @02:57:22.48 Lysis2.in :homing @02:57:22.53 Air.in :homing @02:57:22.60 Plunge.in :homing @02:57:22.65 Lysis1.in :homing @02:57:23.15 PinPort.in :homing @02:57:23.66 Toroid.jog 599 @02:57:23.72 Lysis2.jog 1391 @02:57:23.77 Air.jog 1363 @02:57:23.81 Plunge.jog 1558 @02:57:23.86 Lysis1.jog 1388 @02:57:23.96 PinPort.jog -109 @02:57:24.42 PinPort.setRawPosition! 0 @02:57:24.50 PinPort.seek :home @02:57:24.63 Archive.setRawPosition! 50 @02:57:24.67 PinPort.in :default @02:57:24.82 Archive.seek :home @02:57:25.13 Archive.in :default @02:57:25.26 Air.setRawPosition! 50 @02:57:25.31 Lysis2.setRawPosition! 50 @02:57:25.47 Lysis2.seek :home @02:57:25.52 Air.seek :home @02:57:25.73 Lysis1.setRawPosition! 50 @02:57:25.76 Plunge.setRawPosition! 50 @02:57:25.90 Lysis1.seek :home @02:57:26.00 Plunge.seek :home @02:57:26.20 Air.in :default @02:57:26.26 Toroid.setRawPosition! 0 @02:57:26.30 Lysis2.in :default @02:57:26.47 Toroid.seek :home @02:57:26.62 Plunge.in :default @02:57:26.67 Lysis1.in :default @02:57:26.87 Toroid.in :default @02:57:27.10
Toroid.seek :clear @02:57:28.75 Carousel.in :default @02:57:28.90 Carousel.home.seek 28 @02:57:29.18 Selecting Cartridge 27 @02:57:29.56 Carousel.seek 27 @02:57:30.12 Lysis1.in :fast @02:57:30.22 Lysis1.seek :locked @02:57:32.87 Plunge.seek :locked @02:57:33.07 Air.in :fast @02:57:33.16 Air.seek :locked @02:57:34.75
Carousel.reconfigure :locked @02:57:34.94 Powering cartridge bus @02:57:37.60 Gate.setIR 1750,5000,0,6 @02:57:37.73 Gate.setIR 0,5000,0,6 @02:57:37.82 Gate.power :cartridge,:OFF @02:57:38.40 Mounted archive Cartridge @02:57:38.51 Cartridge::Incompatible in MAIN -- archive sensed when expecting microSPR_phyto in slot 27 @02:57:38.53 Waiting to Resume from Checkpoint "Selecting Cartridge 27" @04:35:01.63 Session Begun @04:35:06.36 -> showlog -20 @04:35:59.95 -> slot {26..20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0500h.spr.bz2}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0422h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0420h.spr.bz2}} @04:37:50.33 -> Cartridge.unlock @04:37:50.47 Carousel.in :default @04:37:50.65 Carousel.home.seek 27 @04:37:51.12 Air.in :unlocking @04:37:51.33 Air.seek :clear @04:37:52.82 Lysis1.in :unlocking @04:37:53.02 Lysis1.seek :clear @04:37:53.14 Plunge.in :unlocking @04:37:53.25 Plunge.seek :clear @04:38:21.21 -> slot {26..20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0500h.spr.bz2}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0422h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0420h.spr.bz2}} @04:40:06.48 -> exit @04:40:06.51 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @04:41:27.99 Session Begun @04:41:35.67 -> showlog @04:42:12.28 -> showlog -10 @04:42:27.56 -> slot {26..20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0500h.spr.bz2}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0422h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0420h.spr.bz2}} @04:42:52.31 -> slot {26..20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0500h.spr.bz2}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0422h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0420h.spr.bz2}} @04:43:00.21 -> showlog @04:43:16.31 -> MainThread.exit # @04:43:16.52 ThreadError in bill#2 -- killed thread @04:43:16.62 Session Ended @04:43:16.67 Session Ended @04:45:43.09PDT05-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @04:45:44.67 >BREAK< ESP3 Gateway boot v1.43 for core bus @04:45:44.69 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @04:45:48.01 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @04:45:48.63 Sea pressure (Abs) tare is 6% of full scale @04:45:51.77 Polling Can every 2:00 @04:45:51.81 Loading /home/esp/esp2/bin/processAll mission @04:45:52.34 Sea pressure (Abs) tare is 6% of full scale @04:45:52.37 Sampling default goal volumes @04:45:52.41 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @04:45:52.73 Gate.power :sampler,:OFF,limit to 3500mA @04:45:52.75 Gate.power :analytic1,:ON,limit to 5000mA @04:45:52.78 Gate.power :analytic2,:ON,limit to 5000mA @04:45:53.52 Session Begun @04:45:53.84 SPR Module 1, FW Rev. B, FPGA Rev. C @04:45:54.12
Archive.configure :default @04:45:54.21 Archive.hold @04:45:54.32 Archive.configure :default @04:45:54.50 Air.configure :default @04:45:54.58 Air.hold @04:45:54.68 Air.configure :default @04:45:54.83 Lysis1.configure :default @04:45:54.92 Lysis1.hold @04:45:55.01 Lysis1.configure :default @04:45:55.16 Lysis2.configure :default @04:45:55.25 Lysis2.hold @04:45:55.35 Lysis2.configure :default @04:45:55.58 Plunge.configure :default @04:45:55.66 Plunge.hold @04:45:55.75 Plunge.configure :default @04:45:55.94 SamplePump.configure :default @04:45:56.13 Toroid.configure :default @04:45:56.22 Toroid.hold @04:45:56.31 Toroid.configure :default @04:45:56.50 Carousel.configure :default @04:45:56.58 Carousel.hold @04:45:56.69 Carousel.configure :default @04:45:56.87 Pressure.configure :default @04:45:57.05 ExtraPump.configure :default @04:45:57.24 PinPort.configure :default @04:45:57.33 PinPort.hold @04:45:57.45 PinPort.configure :default @04:45:57.55 All elf controllers running firmware version 4.84 @04:45:58.36 Toroid.seek :clear @04:45:58.47 Carousel.in :default @04:45:58.62 Carousel.home.seek 27 @04:45:58.92 Selecting Cartridge 26 @04:45:59.26 Carousel.seek 26 @04:46:00.31 Lysis1.in :fast @04:46:00.40 Lysis1.seek :locked @04:46:03.05 Plunge.seek :locked @04:46:03.25 Air.in :fast @04:46:03.34 Air.seek :locked @04:46:04.96
Carousel.reconfigure :locked @04:46:05.15 Powering cartridge bus @04:46:07.82 Gate.setIR 1750,5000,0,6 @04:46:07.95 Gate.setIR 0,5000,0,6 @04:46:08.04 PuckHeater.configure :default @04:46:08.12 Gate.power :heat,:ON,limit to 5000mA @04:46:08.79 Mounted lysis Cartridge @04:46:08.84 WARNING: Using default puck temperature sensor calibration @04:46:08.85 Using default cartridge heat loss curve for HeatingAccelInCarousel @04:46:10.49 SPR.command "read_sequences" @04:46:10.72 SPR.command "stats_on" @04:46:10.89 SPR.command "start 0 1" @04:46:11.59 SPR.start /var/log/esp/spr23aug0504h.spr.bz2 @04:46:14.07 Waiting for baseline SPR stabilization @04:46:14.09 Handoff.select :waste @04:46:20.83 Syringe tare is 19% of full scale @04:46:20.86 PinPort.in :locking @04:46:20.97 PinPort.seek :locked @04:46:23.53 Archive.in :fast @04:46:23.61 Lysis2.in :fast @04:46:23.70 Lysis1.seek 1000uL @04:46:23.89 Archive.seek :full @04:46:23.97 Lysis2.seek 1000uL @04:46:28.54 delayUntil "04:56:14.03" @04:56:14.05 Lysis2.in :DAmix @04:56:14.20 Lysis1.in :DAmix @04:56:14.82 Lysis2.seek 750uL @04:56:14.85 Lysis1.seek 750uL @04:56:49.98 delay 5 seconds @04:56:55.00 Handoff.select SPR @04:56:55.16 SPR.command "start 2 14" @04:56:55.79 SPR stabilized @04:56:55.98 delay 10 seconds @04:57:06.00 SPR.command "start 2 0" @04:57:09.16 Waiting up to 11:00 for SPR to enter state 1 @04:59:06.82 SPR.command "hold_on" @04:59:08.21 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @04:59:08.86 Lysis2.seek 450uL @04:59:08.94 Lysis1.seek 450uL @04:59:50.96 delay 15 seconds @05:00:05.98 SPR.command "hold_off" @05:00:08.86 Waiting up to 38:00 for SPR to enter state 7 @05:14:29.04 SPRsummary:29.4RIU,40.9RIU,104RIU,135RIU after 1792s @05:25:59.63 delay 10 seconds @05:26:09.66 SPR.command "start 2 0" @05:26:12.33 Waiting up to 11:00 for SPR to enter state 1 @05:28:10.00 SPR.command "hold_on" @05:28:11.38 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:28:12.05 Lysis2.seek 300uL @05:28:12.10 Lysis1.seek 300uL @05:28:33.22 delay 15 seconds @05:28:48.24 SPR.command "hold_off" @05:28:51.75 Waiting up to 38:00 for SPR to enter state 7 @05:54:42.94 delay 10 seconds @05:54:52.96 SPR.command "start 2 0" @05:54:56.16 Waiting up to 11:00 for SPR to enter state 1 @05:56:54.26 SPR.command "hold_on" @05:56:55.64 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:56:56.28 Lysis2.seek 150uL @05:56:56.31 Lysis1.seek 150uL @05:57:17.47 delay 15 seconds @05:57:32.49 SPR.command "hold_off" @05:57:35.48 Waiting up to 38:00 for SPR to enter state 7 @06:23:26.24 delay 10 seconds @06:23:36.27 SPR.command "start 2 0" @06:23:38.95 Waiting up to 11:00 for SPR to enter state 1 @06:25:36.62 SPR.command "hold_on" @06:25:38.53 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @06:25:39.12 Lysis2.seek 0uL @06:25:39.17 Lysis1.seek 0uL @06:26:00.32 delay 15 seconds @06:26:15.35 SPR.command "hold_off" @06:26:19.32 Waiting up to 38:00 for SPR to enter state 7 @06:52:09.44 SPR analysis completed @06:52:13.57 Ended log spr23aug0504h.spr.bz2 @06:52:13.60 SPR.command "stats_off" @06:52:14.29 Intake.close @06:52:14.36 Exhaust.close @06:52:14.93 Bypass.close @06:52:15.04 Handoff.select :close @06:52:15.24
Gate.power :heat,:OFF @06:52:15.26 Gate.power :cartridge,:OFF @06:52:15.40 PinPort.in :unlocking @06:52:15.52 PinPort.seek :clear @06:52:17.46 Carousel.in :default @06:52:17.68 Carousel.home.seek 26 @06:52:18.11 Plunge.seek :locked @06:52:18.31 Archive.in :unlocking @06:52:18.50 Archive.seek :clear @06:52:18.56 Air.in :unlocking @06:52:18.79 Air.seek :clear @06:52:18.86 Lysis1.in :unlocking @06:52:19.06 Lysis1.seek :clear @06:52:19.20 Lysis2.in :unlocking @06:52:19.30 Lysis2.seek :clear @06:52:23.93
Gate.power :motor,:OFF @06:52:23.95 Gate.power :core,:OFF @06:52:23.98 Gate.power :analytic1,:OFF @06:52:24.02 Gate.power :analytic2,:OFF @06:52:24.05 Gate.power :main,:OFF @06:52:24.08 Safely stopped and ready to power off @06:52:24.60 Gate.power :sampler,:OFF,limit to 3500mA @06:52:27.79 Gate.power :main,:ON,limit to 5000mA @06:52:27.81 Gate.power :analytic1,:ON,limit to 5000mA @06:52:27.85 Gate.power :analytic2,:ON,limit to 5000mA @06:52:27.95 Powering core bus @06:52:30.30 SPR Module 1, FW Rev. B, FPGA Rev. C @06:52:30.73
Archive.configure :default @06:52:30.85 Air.configure :default @06:52:30.94 Lysis1.configure :default @06:52:31.03 Lysis2.configure :default @06:52:31.14 Plunge.configure :default @06:52:31.25 SamplePump.configure :default @06:52:31.37 Toroid.configure :default @06:52:31.53 Carousel.configure :default @06:52:31.65 Pressure.configure :default @06:52:31.76 ExtraPump.configure :default @06:52:31.85 PinPort.configure :default @06:52:31.94 All elf controllers running firmware version 4.84 @06:52:31.97 Gate.power :motor,:ON,limit to 5000mA @06:52:32.30 Archive.home.jog 0 @06:52:33.06 PinPort.home.jog 0 @06:52:33.12 Toroid.home.jog 0 @06:52:33.16 Plunge.home.jog 0 @06:52:33.20 Lysis1.home.jog 0 @06:52:33.25 Lysis2.home.jog 0 @06:52:33.30 Air.home.jog 0 @06:52:33.33 Carousel.home.jog 0 @06:52:34.79 Archive.in :homing @06:52:35.11 Archive.jog 1382 @06:52:35.19 Toroid.in :homing @06:52:35.25 Lysis2.in :homing @06:52:35.30 Air.in :homing @06:52:35.35 PinPort.in :homing @06:52:35.46 Plunge.in :homing @06:52:35.50 Lysis1.in :homing @06:52:35.86 Toroid.jog 594 @06:52:35.94 Lysis2.jog 1391 @06:52:35.99 Air.jog 1366 @06:52:36.03 PinPort.jog -110 @06:52:36.10 Lysis1.jog 1387 @06:52:36.14 Plunge.jog 1556 @06:52:36.60 PinPort.setRawPosition! 0 @06:52:36.68 PinPort.seek :home @06:52:36.81 Archive.setRawPosition! 50 @06:52:36.85 PinPort.in :default @06:52:36.97 Archive.seek :home @06:52:37.30 Archive.in :default @06:52:37.45 Lysis2.setRawPosition! 50 @06:52:37.55 Air.setRawPosition! 50 @06:52:37.62 Lysis2.seek :home @06:52:37.71 Air.seek :home @06:52:37.87 Lysis1.setRawPosition! 50 @06:52:37.90 Plunge.setRawPosition! 50 @06:52:38.04 Lysis1.seek :home @06:52:38.07 Plunge.seek :home @06:52:38.30 Lysis2.in :default @06:52:38.34 Air.in :default @06:52:38.38 Toroid.setRawPosition! 0 @06:52:38.58 Toroid.seek :home @06:52:38.74 Plunge.in :default @06:52:38.78 Lysis1.in :default @06:52:38.82 Toroid.in :default @06:52:39.29
Toroid.seek :clear @06:52:40.93 Carousel.in :default @06:52:41.10 Carousel.home.seek 26 @06:52:41.43 Selecting Cartridge 25 @06:52:41.80 Carousel.seek 25 @06:52:42.36 Lysis1.in :fast @06:52:42.45 Lysis1.seek :locked @06:52:45.10 Plunge.seek :locked @06:52:45.29 Air.in :fast @06:52:45.37 Air.seek :locked @06:52:46.99
Carousel.reconfigure :locked @06:52:47.18 Powering cartridge bus @06:52:49.83 Gate.setIR 1750,5000,0,6 @06:52:49.96 Gate.setIR 0,5000,0,6 @06:52:50.04 PuckHeater.configure :default @06:52:50.13 Gate.power :heat,:ON,limit to 5000mA @06:52:50.74 Mounted lysis Cartridge @06:52:50.78 WARNING: Using default puck temperature sensor calibration @06:52:50.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @06:52:52.95 SPR.command "read_sequences" @06:52:53.03 SPR.command "stats_on" @06:52:53.92 SPR.command "start 0 1" @06:52:54.62 SPR.start /var/log/esp/spr23aug0506h.spr.bz2 @06:52:56.65 Waiting for baseline SPR stabilization @06:52:56.68 Handoff.select :waste @06:53:03.46 Syringe tare is 19% of full scale @06:53:03.49 PinPort.in :locking @06:53:03.59 PinPort.seek :locked @06:53:06.16 Archive.in :fast @06:53:06.21 Lysis2.in :fast @06:53:06.32 Lysis1.seek 1000uL @06:53:06.44 Archive.seek :full @06:53:06.66 Lysis2.seek 1000uL @06:53:11.25 delayUntil "07:02:56.22" @07:02:56.24 Lysis2.in :DAmix @07:02:56.40 Lysis1.in :DAmix @07:02:56.96 Lysis2.seek 750uL @07:02:57.01 Lysis1.seek 750uL @07:03:32.18 delay 5 seconds @07:03:37.20 Handoff.select SPR @07:03:37.35 SPR.command "start 2 14" @07:03:37.94 SPR stabilized @07:03:38.12 delay 10 seconds @07:03:48.14 SPR.command "start 2 0" @07:03:51.30 Waiting up to 11:00 for SPR to enter state 1 @07:05:20.93 Session Ended @07:05:48.98 SPR.command "hold_on" @07:05:50.36 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:05:50.98 Lysis2.seek 450uL @07:05:51.01 Lysis1.seek 450uL @07:06:33.06 delay 15 seconds @07:06:48.08 SPR.command "hold_off" @07:06:51.01 Waiting up to 38:00 for SPR to enter state 7 @07:21:11.19 SPRsummary:13.1RIU,21.4RIU,85.7RIU,113RIU after 1791s @07:32:41.79 delay 10 seconds @07:32:51.81 SPR.command "start 2 0" @07:32:54.48 Waiting up to 11:00 for SPR to enter state 1 @07:34:52.15 SPR.command "hold_on" @07:34:53.53 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @07:34:54.11 Lysis2.seek 300uL @07:34:54.16 Lysis1.seek 300uL @07:35:15.28 delay 15 seconds @07:35:30.30 SPR.command "hold_off" @07:35:33.38 Waiting up to 38:00 for SPR to enter state 7 @08:01:24.13 delay 10 seconds @08:01:34.15 SPR.command "start 2 0" @08:01:37.36 Waiting up to 11:00 for SPR to enter state 1 @08:03:35.91 SPR.command "hold_on" @08:03:36.84 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:03:37.43 Lysis2.seek 150uL @08:03:37.47 Lysis1.seek 150uL @08:03:58.59 delay 15 seconds @08:04:13.61 SPR.command "hold_off" @08:04:16.69 Waiting up to 38:00 for SPR to enter state 7 @08:11:23.43 Session Begun @08:11:28.75 -> showlog @08:30:07.48 delay 10 seconds @08:30:17.50 SPR.command "start 2 0" @08:30:20.15 Waiting up to 11:00 for SPR to enter state 1 @08:32:17.83 SPR.command "hold_on" @08:32:19.21 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:32:19.94 Lysis2.seek 0uL @08:32:20.02 Lysis1.seek 0uL @08:32:41.13 delay 15 seconds @08:32:56.15 SPR.command "hold_off" @08:32:59.05 Waiting up to 38:00 for SPR to enter state 7 @08:43:44.45 Session Ended @08:58:49.67 SPR analysis completed @08:58:53.63 Ended log spr23aug0506h.spr.bz2 @08:58:53.65 SPR.command "stats_off" @08:58:54.06 Handoff.select :close @08:58:54.21
Gate.power :heat,:OFF @08:58:54.23 Gate.power :cartridge,:OFF @08:58:54.31 PinPort.in :unlocking @08:58:54.37 PinPort.seek :clear @08:58:56.29 Carousel.in :default @08:58:56.41 Carousel.home.seek 25 @08:58:56.75 Plunge.seek :locked @08:58:57.36 Archive.in :unlocking @08:58:57.57 Archive.seek :clear @08:58:57.66 Air.in :unlocking @08:58:57.82 Air.seek :clear @08:58:57.93 Lysis1.in :unlocking @08:58:58.10 Lysis1.seek :clear @08:58:58.16 Lysis2.in :unlocking @08:58:58.27 Lysis2.seek :clear @08:59:02.88
Gate.power :motor,:OFF @08:59:02.89 Gate.power :core,:OFF @08:59:02.90 Gate.power :analytic1,:OFF @08:59:02.92 Gate.power :analytic2,:OFF @08:59:02.93 Gate.power :main,:OFF @08:59:02.94 Safely stopped and ready to power off @08:59:03.40 Gate.power :sampler,:OFF,limit to 3500mA @08:59:06.71 Gate.power :main,:ON,limit to 5000mA @08:59:06.73 Gate.power :analytic1,:ON,limit to 5000mA @08:59:06.74 Gate.power :analytic2,:ON,limit to 5000mA @08:59:06.81 Powering core bus @08:59:09.18 SPR Module 1, FW Rev. B, FPGA Rev. C @08:59:09.59
Archive.configure :default @08:59:09.69 Air.configure :default @08:59:09.75 Lysis1.configure :default @08:59:09.82 Lysis2.configure :default @08:59:09.91 Plunge.configure :default @08:59:10.00 SamplePump.configure :default @08:59:10.10 Toroid.configure :default @08:59:10.19 Carousel.configure :default @08:59:10.28 Pressure.configure :default @08:59:10.37 ExtraPump.configure :default @08:59:10.44 PinPort.configure :default @08:59:10.50 All elf controllers running firmware version 4.84 @08:59:10.52 Gate.power :motor,:ON,limit to 5000mA @08:59:10.73 Archive.home.jog 0 @08:59:11.55 PinPort.home.jog 0 @08:59:11.61 Carousel.home.jog 0 @08:59:11.64 Plunge.home.jog 0 @08:59:11.67 Lysis2.home.jog 0 @08:59:11.69 Air.home.jog 0 @08:59:11.73 Toroid.home.jog 0 @08:59:11.78 Lysis1.home.jog 0 @08:59:12.59 Archive.in :homing @08:59:12.85 Plunge.in :homing @08:59:12.96 Archive.jog 1385 @08:59:13.00 Toroid.in :homing @08:59:13.02 Lysis1.in :homing @08:59:13.06 PinPort.in :homing @08:59:13.10 Lysis2.in :homing @08:59:13.13 Air.in :homing @08:59:13.50 Plunge.jog 1555 @08:59:13.56 Toroid.jog 589 @08:59:13.59 Lysis1.jog 1391 @08:59:13.62 Lysis2.jog 1391 @08:59:13.65 PinPort.jog -109 @08:59:13.68 Air.jog 1365 @08:59:14.66 PinPort.setRawPosition! 0 @08:59:14.68 Archive.setRawPosition! 50 @08:59:14.77 PinPort.seek :home @08:59:14.81 Archive.seek :home @08:59:14.99 PinPort.in :default @08:59:15.15 Plunge.setRawPosition! 50 @08:59:15.19 Lysis2.setRawPosition! 50 @08:59:15.21 Lysis1.setRawPosition! 50 @08:59:15.23 Air.setRawPosition! 50 @08:59:15.37 Lysis2.seek :home @08:59:15.48 Plunge.seek :home @08:59:15.56 Lysis1.seek :home @08:59:15.60 Air.seek :home @08:59:15.90 Lysis2.in :default @08:59:15.93 Archive.in :default @08:59:15.97 Toroid.setRawPosition! 0 @08:59:16.12 Toroid.seek :home @08:59:16.27 Plunge.in :default @08:59:16.32 Lysis1.in :default @08:59:16.35 Toroid.in :default @08:59:16.41 Air.in :default @08:59:16.88
Toroid.seek :clear @08:59:18.50 Carousel.in :default @08:59:18.63 Carousel.home.seek 25 @08:59:18.88 Selecting Cartridge 24 @08:59:19.19 Carousel.seek 24 @08:59:19.74 Lysis1.in :fast @08:59:19.80 Lysis1.seek :locked @08:59:22.43 Plunge.seek :locked @08:59:22.59 Air.in :fast @08:59:22.66 Air.seek :locked @08:59:24.23
Carousel.reconfigure :locked @08:59:24.39 Powering cartridge bus @08:59:27.04 Gate.setIR 1750,5000,0,6 @08:59:27.17 Gate.setIR 0,5000,0,6 @08:59:27.23 PuckHeater.configure :default @08:59:27.29 Gate.power :heat,:ON,limit to 5000mA @08:59:27.90 Mounted lysis Cartridge @08:59:27.93 WARNING: Using default puck temperature sensor calibration @08:59:27.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:59:29.49 SPR.command "read_sequences" @08:59:29.86 SPR.command "stats_on" @08:59:30.01 SPR.command "start 0 1" @08:59:30.72 SPR.start /var/log/esp/spr23aug0508h.spr.bz2 @08:59:33.23 Waiting for baseline SPR stabilization @08:59:33.25 Handoff.select :waste @08:59:39.95 Syringe tare is 19% of full scale @08:59:39.97 PinPort.in :locking @08:59:40.04 PinPort.seek :locked @08:59:42.59 Archive.in :fast @08:59:42.65 Lysis2.in :fast @08:59:42.74 Lysis1.seek 1000uL @08:59:42.89 Archive.seek :full @08:59:42.99 Lysis2.seek 1000uL @08:59:47.50 delayUntil "09:09:33.20" @09:09:33.21 Lysis2.in :DAmix @09:09:33.35 Lysis1.in :DAmix @09:09:33.88 Lysis2.seek 750uL @09:09:33.94 Lysis1.seek 750uL @09:10:09.05 delay 5 seconds @09:10:14.07 Handoff.select SPR @09:10:14.19 SPR.command "start 2 14" @09:10:15.47 SPR stabilized @09:10:15.62 delay 10 seconds @09:10:25.64 SPR.command "start 2 0" @09:10:28.30 Waiting up to 11:00 for SPR to enter state 1 @09:12:25.98 SPR.command "hold_on" @09:12:27.36 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:12:27.99 Lysis2.seek 450uL @09:12:28.06 Lysis1.seek 450uL @09:13:10.14 delay 15 seconds @09:13:25.16 SPR.command "hold_off" @09:13:28.01 Waiting up to 38:00 for SPR to enter state 7 @09:27:48.19 SPRsummary:17RIU,25.3RIU,87.3RIU,110RIU after 1792s @09:39:18.78 delay 10 seconds @09:39:28.81 SPR.command "start 2 0" @09:39:31.48 Waiting up to 11:00 for SPR to enter state 1 @09:41:29.15 SPR.command "hold_on" @09:41:30.53 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @09:41:31.11 Lysis2.seek 300uL @09:41:31.18 Lysis1.seek 300uL @09:41:52.27 delay 15 seconds @09:42:07.29 SPR.command "hold_off" @09:42:10.38 Waiting up to 38:00 for SPR to enter state 7 @10:08:01.13 delay 10 seconds @10:08:11.16 SPR.command "start 2 0" @10:08:13.84 Waiting up to 11:00 for SPR to enter state 1 @10:10:11.50 SPR.command "hold_on" @10:10:12.90 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:10:13.49 Lysis2.seek 150uL @10:10:13.53 Lysis1.seek 150uL @10:10:34.62 delay 15 seconds @10:10:49.64 SPR.command "hold_off" @10:10:53.26 Waiting up to 38:00 for SPR to enter state 7 @10:36:44.45 delay 10 seconds @10:36:54.47 SPR.command "start 2 0" @10:36:57.15 Waiting up to 11:00 for SPR to enter state 1 @10:38:54.81 SPR.command "hold_on" @10:38:56.20 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:38:56.75 Lysis2.seek 0uL @10:38:56.81 Lysis1.seek 0uL @10:39:17.90 delay 15 seconds @10:39:32.92 SPR.command "hold_off" @10:39:36.05 Waiting up to 38:00 for SPR to enter state 7 @11:05:26.66 SPR analysis completed @11:05:30.62 Ended log spr23aug0508h.spr.bz2 @11:05:30.64 SPR.command "stats_off" @11:05:31.04 Handoff.select :close @11:05:31.19
Gate.power :heat,:OFF @11:05:31.21 Gate.power :cartridge,:OFF @11:05:31.74 PinPort.in :unlocking @11:05:31.81 PinPort.seek :clear @11:05:33.73 Carousel.in :default @11:05:33.86 Carousel.home.seek 24 @11:05:34.19 Plunge.seek :locked @11:05:34.36 Archive.in :unlocking @11:05:34.53 Archive.seek :clear @11:05:34.61 Air.in :unlocking @11:05:34.79 Air.seek :clear @11:05:34.92 Lysis1.in :unlocking @11:05:35.05 Lysis1.seek :clear @11:05:35.11 Lysis2.in :unlocking @11:05:35.23 Lysis2.seek :clear @11:05:39.83
Gate.power :motor,:OFF @11:05:39.84 Gate.power :core,:OFF @11:05:39.86 Gate.power :analytic1,:OFF @11:05:39.87 Gate.power :analytic2,:OFF @11:05:39.88 Gate.power :main,:OFF @11:05:39.90 Safely stopped and ready to power off @11:05:40.33 Gate.power :sampler,:OFF,limit to 3500mA @11:05:43.67 Gate.power :main,:ON,limit to 5000mA @11:05:43.68 Gate.power :analytic1,:ON,limit to 5000mA @11:05:43.70 Gate.power :analytic2,:ON,limit to 5000mA @11:05:43.76 Powering core bus @11:05:46.13 SPR Module 1, FW Rev. B, FPGA Rev. C @11:05:46.53
Archive.configure :default @11:05:46.63 Air.configure :default @11:05:46.69 Lysis1.configure :default @11:05:46.76 Lysis2.configure :default @11:05:46.85 Plunge.configure :default @11:05:46.94 SamplePump.configure :default @11:05:47.04 Toroid.configure :default @11:05:47.13 Carousel.configure :default @11:05:47.22 Pressure.configure :default @11:05:47.31 ExtraPump.configure :default @11:05:47.38 PinPort.configure :default @11:05:47.47 All elf controllers running firmware version 4.84 @11:05:47.49 Gate.power :motor,:ON,limit to 5000mA @11:05:47.79 Archive.home.jog 0 @11:05:48.51 PinPort.home.jog 0 @11:05:48.57 Toroid.home.jog 0 @11:05:48.61 Plunge.home.jog 0 @11:05:48.64 Lysis1.home.jog 0 @11:05:48.67 Lysis2.home.jog 0 @11:05:48.70 Air.home.jog 0 @11:05:48.72 Carousel.home.jog 0 @11:05:49.58 Archive.in :homing @11:05:50.47 Archive.jog 1386 @11:05:50.55 Toroid.in :homing @11:05:50.58 Lysis2.in :homing @11:05:50.61 Air.in :homing @11:05:50.66 PinPort.in :homing @11:05:50.71 Plunge.in :homing @11:05:50.77 Lysis1.in :homing @11:05:51.01 Lysis2.jog 1392 @11:05:51.11 Toroid.jog 584 @11:05:51.24 Lysis1.jog 1385 @11:05:51.26 PinPort.jog -110 @11:05:51.28 Plunge.jog 1555 @11:05:51.32 Air.jog 1364 @11:05:51.75 PinPort.setRawPosition! 0 @11:05:51.80 PinPort.seek :home @11:05:51.90 PinPort.in :default @11:05:51.98 Archive.setRawPosition! 50 @11:05:52.04 Archive.seek :home @11:05:52.25 Lysis2.setRawPosition! 50 @11:05:52.32 Archive.in :default @11:05:52.37 Lysis2.seek :home @11:05:52.64 Lysis1.setRawPosition! 50 @11:05:52.70 Lysis1.seek :home @11:05:52.82 Plunge.setRawPosition! 50 @11:05:52.85 Lysis2.in :default @11:05:52.88 Air.setRawPosition! 50 @11:05:53.03 Plunge.seek :home @11:05:53.07 Air.seek :home @11:05:53.25 Lysis1.in :default @11:05:53.44 Plunge.in :default @11:05:53.47 Toroid.setRawPosition! 0 @11:05:53.58 Toroid.seek :home @11:05:53.69 Air.in :default @11:05:53.72 Toroid.in :default @11:05:54.10
Toroid.seek :clear @11:05:55.71 Carousel.in :default @11:05:55.84 Carousel.home.seek 24 @11:05:56.10 Selecting Cartridge 23 @11:05:56.41 Carousel.seek 23 @11:05:56.95 Lysis1.in :fast @11:05:57.01 Lysis1.seek :locked @11:05:59.64 Plunge.seek :locked @11:05:59.80 Air.in :fast @11:05:59.87 Air.seek :locked @11:06:01.46
Carousel.reconfigure :locked @11:06:01.63 Powering cartridge bus @11:06:04.47 Gate.setIR 1750,5000,0,6 @11:06:04.60 Gate.setIR 0,5000,0,6 @11:06:04.67 PuckHeater.configure :default @11:06:04.73 Gate.power :heat,:ON,limit to 5000mA @11:06:05.34 Mounted lysis Cartridge @11:06:05.36 WARNING: Using default puck temperature sensor calibration @11:06:05.38 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:06:06.98 SPR.command "read_sequences" @11:06:07.76 SPR.command "stats_on" @11:06:07.93 SPR.command "start 0 1" @11:06:08.63 SPR.start /var/log/esp/spr23aug0511h.spr.bz2 @11:06:10.62 Waiting for baseline SPR stabilization @11:06:10.64 Handoff.select :waste @11:06:17.34 Syringe tare is 19% of full scale @11:06:17.36 PinPort.in :locking @11:06:17.46 PinPort.seek :locked @11:06:20.00 Archive.in :fast @11:06:20.08 Lysis2.in :fast @11:06:20.12 Lysis1.seek 1000uL @11:06:20.26 Archive.seek :full @11:06:20.34 Lysis2.seek 1000uL @11:06:24.92 delayUntil "11:16:10.18" @11:16:10.20 Lysis2.in :DAmix @11:16:10.32 Lysis1.in :DAmix @11:16:10.88 Lysis2.seek 750uL @11:16:10.94 Lysis1.seek 750uL @11:16:46.08 delay 5 seconds @11:16:51.10 Handoff.select SPR @11:16:51.23 SPR.command "start 2 14" @11:16:51.89 SPR stabilized @11:16:52.05 delay 10 seconds @11:17:02.07 SPR.command "start 2 0" @11:17:05.26 Waiting up to 11:00 for SPR to enter state 1 @11:19:02.93 SPR.command "hold_on" @11:19:04.31 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:19:04.91 Lysis2.seek 450uL @11:19:04.97 Lysis1.seek 450uL @11:19:47.00 delay 15 seconds @11:20:02.03 SPR.command "hold_off" @11:20:04.97 Waiting up to 38:00 for SPR to enter state 7 @11:34:25.14 SPRsummary:13.5RIU,21.8RIU,85.7RIU,103RIU after 1791s @11:45:55.72 delay 10 seconds @11:46:05.75 SPR.command "start 2 0" @11:46:08.43 Waiting up to 11:00 for SPR to enter state 1 @11:48:06.10 SPR.command "hold_on" @11:48:08.00 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @11:48:08.57 Lysis2.seek 300uL @11:48:08.62 Lysis1.seek 300uL @11:48:29.73 delay 15 seconds @11:48:44.76 SPR.command "hold_off" @11:48:47.31 Waiting up to 38:00 for SPR to enter state 7 @12:14:37.14 delay 10 seconds @12:14:47.16 SPR.command "start 2 0" @12:14:49.84 Waiting up to 11:00 for SPR to enter state 1 @12:16:47.50 SPR.command "hold_on" @12:16:49.45 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:16:50.01 Lysis2.seek 150uL @12:16:50.09 Lysis1.seek 150uL @12:17:11.16 delay 15 seconds @12:17:26.20 SPR.command "hold_off" @12:17:29.25 Waiting up to 38:00 for SPR to enter state 7 @12:43:19.53 delay 10 seconds @12:43:29.55 SPR.command "start 2 0" @12:43:32.71 Waiting up to 11:00 for SPR to enter state 1 @12:45:30.82 SPR.command "hold_on" @12:45:32.20 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:45:32.78 Lysis2.seek 0uL @12:45:32.83 Lysis1.seek 0uL @12:45:53.93 delay 15 seconds @12:46:08.95 SPR.command "hold_off" @12:46:12.04 Waiting up to 38:00 for SPR to enter state 7 @13:12:02.65 SPR analysis completed @13:12:06.74 Ended log spr23aug0511h.spr.bz2 @13:12:06.76 SPR.command "stats_off" @13:12:07.04 Handoff.select :close @13:12:07.18
Gate.power :heat,:OFF @13:12:07.20 Gate.power :cartridge,:OFF @13:12:07.75 PinPort.in :unlocking @13:12:07.81 PinPort.seek :clear @13:12:09.73 Carousel.in :default @13:12:09.86 Carousel.home.seek 23 @13:12:10.20 Plunge.seek :locked @13:12:10.38 Archive.in :unlocking @13:12:10.52 Archive.seek :clear @13:12:10.58 Air.in :unlocking @13:12:10.78 Air.seek :clear @13:12:10.90 Lysis1.in :unlocking @13:12:11.08 Lysis1.seek :clear @13:12:11.16 Lysis2.in :unlocking @13:12:11.27 Lysis2.seek :clear @13:12:15.87
Gate.power :motor,:OFF @13:12:15.88 Gate.power :core,:OFF @13:12:15.89 Gate.power :analytic1,:OFF @13:12:15.91 Gate.power :analytic2,:OFF @13:12:15.92 Gate.power :main,:OFF @13:12:15.93 Safely stopped and ready to power off @13:12:16.13 Gate.power :sampler,:OFF,limit to 3500mA @13:12:19.71 Gate.power :main,:ON,limit to 5000mA @13:12:19.72 Gate.power :analytic1,:ON,limit to 5000mA @13:12:19.74 Gate.power :analytic2,:ON,limit to 5000mA @13:12:19.80 Powering core bus @13:12:22.17 SPR Module 1, FW Rev. B, FPGA Rev. C @13:12:22.57
Archive.configure :default @13:12:22.67 Air.configure :default @13:12:22.73 Lysis1.configure :default @13:12:22.80 Lysis2.configure :default @13:12:22.89 Plunge.configure :default @13:12:22.99 SamplePump.configure :default @13:12:23.08 Toroid.configure :default @13:12:23.17 Carousel.configure :default @13:12:23.26 Pressure.configure :default @13:12:23.36 ExtraPump.configure :default @13:12:23.45 PinPort.configure :default @13:12:23.52 All elf controllers running firmware version 4.84 @13:12:23.54 Gate.power :motor,:ON,limit to 5000mA @13:12:24.01 Archive.home.jog 0 @13:12:24.55 PinPort.home.jog 0 @13:12:24.61 Toroid.home.jog 0 @13:12:24.65 Plunge.home.jog 0 @13:12:24.68 Lysis1.home.jog 0 @13:12:24.71 Air.home.jog 0 @13:12:24.74 Carousel.home.jog 0 @13:12:24.78 Lysis2.home.jog 0 @13:12:26.14 Archive.in :homing @13:12:26.50 Toroid.in :homing @13:12:26.58 Plunge.in :homing @13:12:26.62 Lysis1.in :homing @13:12:26.67 Archive.jog 1384 @13:12:26.70 PinPort.in :homing @13:12:26.74 Air.in :homing @13:12:26.80 Lysis2.in :homing @13:12:27.07 Toroid.jog 581 @13:12:27.16 Plunge.jog 1555 @13:12:27.20 Lysis1.jog 1388 @13:12:27.26 PinPort.jog -109 @13:12:27.33 Air.jog 1365 @13:12:27.49 Lysis2.jog 1389 @13:12:27.77 PinPort.setRawPosition! 0 @13:12:27.82 PinPort.seek :home @13:12:27.91 PinPort.in :default @13:12:28.06 Archive.setRawPosition! 50 @13:12:28.12 Archive.seek :home @13:12:28.39 Archive.in :default @13:12:28.59 Lysis1.setRawPosition! 50 @13:12:28.66 Plunge.setRawPosition! 50 @13:12:28.70 Lysis1.seek :home @13:12:28.82 Plunge.seek :home @13:12:28.94 Lysis2.setRawPosition! 50 @13:12:28.97 Air.setRawPosition! 50 @13:12:29.08 Lysis2.seek :home @13:12:29.11 Air.seek :home @13:12:29.31 Toroid.setRawPosition! 0 @13:12:29.34 Plunge.in :default @13:12:29.38 Lysis1.in :default @13:12:29.49 Toroid.seek :home @13:12:29.73 Lysis2.in :default @13:12:29.77 Air.in :default @13:12:29.83 Toroid.in :default @13:12:30.21
Toroid.seek :clear @13:12:31.83 Carousel.in :default @13:12:31.96 Carousel.home.seek 23 @13:12:32.24 Selecting Cartridge 22 @13:12:32.55 Carousel.seek 22 @13:12:33.08 Lysis1.in :fast @13:12:33.14 Lysis1.seek :locked @13:12:35.77 Plunge.seek :locked @13:12:35.93 Air.in :fast @13:12:36.00 Air.seek :locked @13:12:37.95
Carousel.reconfigure :locked @13:12:38.21 Powering cartridge bus @13:12:40.86 Gate.setIR 1750,5000,0,6 @13:12:40.98 Gate.setIR 0,5000,0,6 @13:12:41.05 PuckHeater.configure :default @13:12:41.11 Gate.power :heat,:ON,limit to 5000mA @13:12:41.72 Mounted lysis Cartridge @13:12:41.75 WARNING: Using default puck temperature sensor calibration @13:12:41.76 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:12:43.32 SPR.command "read_sequences" @13:12:43.80 SPR.command "stats_on" @13:12:43.97 SPR.command "start 0 1" @13:12:44.67 SPR.start /var/log/esp/spr23aug0513h.spr.bz2 @13:12:46.66 Waiting for baseline SPR stabilization @13:12:46.67 Handoff.select :waste @13:12:53.38 Syringe tare is 19% of full scale @13:12:53.44 PinPort.in :locking @13:12:53.50 PinPort.seek :locked @13:12:56.04 Archive.in :fast @13:12:56.10 Lysis2.in :fast @13:12:56.17 Lysis1.seek 1000uL @13:12:56.37 Lysis2.seek 1000uL @13:12:56.41 Archive.seek :full @13:13:00.97 delayUntil "13:22:46.22" @13:22:46.24 Lysis2.in :DAmix @13:22:46.37 Lysis1.in :DAmix @13:22:46.94 Lysis2.seek 750uL @13:22:46.99 Lysis1.seek 750uL @13:23:22.09 delay 5 seconds @13:23:27.11 Handoff.select SPR @13:23:27.24 SPR.command "start 2 14" @13:23:27.92 SPR stabilized @13:23:28.09 delay 10 seconds @13:23:38.11 SPR.command "start 2 0" @13:23:41.29 Waiting up to 11:00 for SPR to enter state 1 @13:25:38.96 SPR.command "hold_on" @13:25:40.87 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @13:25:41.49 Lysis2.seek 450uL @13:25:41.53 Lysis1.seek 450uL @13:26:23.59 delay 15 seconds @13:26:38.61 SPR.command "hold_off" @13:26:41.96 Waiting up to 38:00 for SPR to enter state 7 @13:41:01.18 SPRsummary:16.6RIU,24.2RIU,86.5RIU,104RIU after 1791s @13:52:31.76 delay 10 seconds @13:52:41.79 SPR.command "start 2 0" @13:52:44.46 Waiting up to 11:00 for SPR to enter state 1 @13:54:42.13 SPR.command "hold_on" @13:54:43.51 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @13:54:44.11 Lysis2.seek 300uL @13:54:44.17 Lysis1.seek 300uL @13:55:05.24 delay 15 seconds @13:55:20.26 SPR.command "hold_off" @13:55:23.36 Waiting up to 38:00 for SPR to enter state 7 @14:00:24.46 Session Begun @14:00:26.73 -> showlog @14:21:14.12 delay 10 seconds @14:21:24.14 SPR.command "start 2 0" @14:21:26.82 Waiting up to 11:00 for SPR to enter state 1 @14:23:24.49 SPR.command "hold_on" @14:23:25.87 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @14:23:26.59 Lysis2.seek 150uL @14:23:26.69 Lysis1.seek 150uL @14:23:47.79 delay 15 seconds @14:24:02.82 SPR.command "hold_off" @14:24:06.24 Waiting up to 38:00 for SPR to enter state 7 @14:49:57.44 delay 10 seconds @14:50:07.47 SPR.command "start 2 0" @14:50:10.12 Waiting up to 11:00 for SPR to enter state 1 @14:52:07.78 SPR.command "hold_on" @14:52:09.17 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @14:52:09.91 Lysis2.seek 0uL @14:52:09.98 Lysis1.seek 0uL @14:52:31.09 delay 15 seconds @14:52:46.11 SPR.command "hold_off" @14:52:49.02 Waiting up to 38:00 for SPR to enter state 7 @15:18:39.63 SPR analysis completed @15:18:43.76 Ended log spr23aug0513h.spr.bz2 @15:18:43.79 SPR.command "stats_off" @15:18:44.52 Handoff.select :close @15:18:44.69
Gate.power :heat,:OFF @15:18:44.71 Gate.power :cartridge,:OFF @15:18:44.83 PinPort.in :unlocking @15:18:44.92 PinPort.seek :clear @15:18:46.87 Carousel.in :default @15:18:47.03 Carousel.home.seek 22 @15:18:47.38 Plunge.seek :locked @15:18:48.09 Archive.in :unlocking @15:18:48.28 Archive.seek :clear @15:18:48.39 Air.in :unlocking @15:18:48.58 Air.seek :clear @15:18:48.64 Lysis1.in :unlocking @15:18:48.88 Lysis1.seek :clear @15:18:48.94 Lysis2.in :unlocking @15:18:49.11 Lysis2.seek :clear @15:18:53.74
Gate.power :motor,:OFF @15:18:53.77 Gate.power :core,:OFF @15:18:53.80 Gate.power :analytic1,:OFF @15:18:53.84 Gate.power :analytic2,:OFF @15:18:53.88 Gate.power :main,:OFF @15:18:53.90 Safely stopped and ready to power off @15:18:54.12 Gate.power :sampler,:OFF,limit to 3500mA @15:18:57.61 Gate.power :main,:ON,limit to 5000mA @15:18:57.65 Gate.power :analytic1,:ON,limit to 5000mA @15:18:57.67 Gate.power :analytic2,:ON,limit to 5000mA @15:18:57.78 Powering core bus @15:19:00.13 SPR Module 1, FW Rev. B, FPGA Rev. C @15:19:00.56
Archive.configure :default @15:19:00.68 Air.configure :default @15:19:00.76 Lysis1.configure :default @15:19:00.85 Lysis2.configure :default @15:19:00.98 Plunge.configure :default @15:19:01.10 SamplePump.configure :default @15:19:01.21 Toroid.configure :default @15:19:01.33 Carousel.configure :default @15:19:01.48 Pressure.configure :default @15:19:01.60 ExtraPump.configure :default @15:19:01.68 PinPort.configure :default @15:19:01.78 All elf controllers running firmware version 4.84 @15:19:01.80 Gate.power :motor,:ON,limit to 5000mA @15:19:02.07 Archive.home.jog 0 @15:19:02.79 Air.home.jog 0 @15:19:02.89 Carousel.home.jog 0 @15:19:02.97 Plunge.home.jog 0 @15:19:03.01 Lysis2.home.jog 0 @15:19:03.05 Toroid.home.jog 0 @15:19:03.10 Lysis1.home.jog 0 @15:19:03.14 PinPort.home.jog 0 @15:19:03.97 Archive.in :homing @15:19:04.39 Archive.jog 1381 @15:19:04.47 Toroid.in :homing @15:19:04.53 Lysis2.in :homing @15:19:04.58 Air.in :homing @15:19:04.63 Plunge.in :homing @15:19:04.69 Lysis1.in :homing @15:19:04.75 PinPort.in :homing @15:19:05.69 Toroid.jog 589 @15:19:05.74 Plunge.jog 1556 @15:19:05.81 Lysis2.jog 1388 @15:19:05.89 Air.jog 1365 @15:19:06.00 Lysis1.jog 1385 @15:19:06.10 PinPort.jog -110 @15:19:06.50 PinPort.setRawPosition! 0 @15:19:06.61 Archive.setRawPosition! 50 @15:19:06.64 PinPort.seek :home @15:19:06.80 Archive.seek :home @15:19:06.91 PinPort.in :default @15:19:07.09 Archive.in :default @15:19:07.25 Plunge.setRawPosition! 50 @15:19:07.33 Plunge.seek :home @15:19:07.51 Lysis2.setRawPosition! 50 @15:19:07.54 Lysis1.setRawPosition! 50 @15:19:07.58 Air.setRawPosition! 50 @15:19:07.75 Lysis2.seek :home @15:19:07.79 Lysis1.seek :home @15:19:07.89 Air.seek :home @15:19:08.15 Toroid.setRawPosition! 0 @15:19:08.19 Plunge.in :default @15:19:08.34 Toroid.seek :home @15:19:08.50 Lysis2.in :default @15:19:08.54 Lysis1.in :default @15:19:08.58 Toroid.in :default @15:19:08.65 Air.in :default @15:19:09.15
Toroid.seek :clear @15:19:10.80 Carousel.in :default @15:19:10.96 Carousel.home.seek 22 @15:19:11.25 Selecting Cartridge 21 @15:19:11.64 Carousel.seek 21 @15:19:12.21 Lysis1.in :fast @15:19:12.30 Lysis1.seek :locked @15:19:14.95 Plunge.seek :locked @15:19:15.14 Air.in :fast @15:19:15.23 Air.seek :locked @15:19:16.84
Carousel.reconfigure :locked @15:19:17.04 Powering cartridge bus @15:19:19.69 Gate.setIR 1750,5000,0,6 @15:19:19.82 Gate.setIR 0,5000,0,6 @15:19:19.91 PuckHeater.configure :default @15:19:19.99 Gate.power :heat,:ON,limit to 5000mA @15:19:20.60 Mounted lysis Cartridge @15:19:20.65 WARNING: Using default puck temperature sensor calibration @15:19:21.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:19:22.83 SPR.command "read_sequences" @15:19:23.63 SPR.command "stats_on" @15:19:23.82 SPR.command "start 0 1" @15:19:24.52 SPR.start /var/log/esp/spr23aug0515h.spr.bz2 @15:19:26.49 Waiting for baseline SPR stabilization @15:19:26.51 Handoff.select :waste @15:19:33.26 Syringe tare is 19% of full scale @15:19:33.29 PinPort.in :locking @15:19:33.42 PinPort.seek :locked @15:19:35.98 Archive.in :fast @15:19:36.05 Lysis2.in :fast @15:19:36.18 Lysis1.seek 1000uL @15:19:36.30 Archive.seek :full @15:19:36.40 Lysis2.seek 1000uL @15:19:41.00 delayUntil "15:29:26.05" @15:29:26.06 Lysis2.in :DAmix @15:29:26.22 Lysis1.in :DAmix @15:29:26.79 Lysis2.seek 750uL @15:29:26.88 Lysis1.seek 750uL @15:30:02.01 delay 5 seconds @15:30:07.03 Handoff.select SPR @15:30:07.18 SPR.command "start 2 14" @15:30:07.76 SPR stabilized @15:30:07.94 delay 10 seconds @15:30:17.97 SPR.command "start 2 0" @15:30:21.12 Waiting up to 11:00 for SPR to enter state 1 @15:32:18.79 SPR.command "hold_on" @15:32:20.70 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:32:21.34 Lysis2.seek 450uL @15:32:21.38 Lysis1.seek 450uL @15:33:03.52 delay 15 seconds @15:33:18.54 SPR.command "hold_off" @15:33:22.30 Waiting up to 38:00 for SPR to enter state 7 @15:47:41.95 SPRsummary:13.3RIU,21.3RIU,84.1RIU,106RIU after 1792s @15:59:12.97 delay 10 seconds @15:59:23.00 SPR.command "start 2 0" @15:59:26.18 Waiting up to 11:00 for SPR to enter state 1 @16:01:23.84 SPR.command "hold_on" @16:01:25.23 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:01:25.87 Lysis2.seek 300uL @16:01:25.95 Lysis1.seek 300uL @16:01:47.08 delay 15 seconds @16:02:02.10 SPR.command "hold_off" @16:02:05.08 Waiting up to 38:00 for SPR to enter state 7 @16:27:55.84 delay 10 seconds @16:28:05.86 SPR.command "start 2 0" @16:28:08.53 Waiting up to 11:00 for SPR to enter state 1 @16:30:06.21 SPR.command "hold_on" @16:30:07.59 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:30:08.28 Lysis2.seek 150uL @16:30:08.34 Lysis1.seek 150uL @16:30:29.49 delay 15 seconds @16:30:44.51 SPR.command "hold_off" @16:30:47.96 Waiting up to 38:00 for SPR to enter state 7 @16:35:32.14 Session Begun @16:35:34.97 -> showlog @16:35:38.72 -> exit @16:35:38.74 Session Ended @16:37:36.22 Session Begun @16:37:43.52 -> slot {20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 21 => { Type: :microSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr23aug0515h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0513h.spr.bz2}, 23 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0511h.spr.bz2}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0508h.spr.bz2}, 25 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0506h.spr.bz2}, 26 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0504h.spr.bz2}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0500h.spr.bz2"}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0422h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0420h.spr.bz2"}} @16:51:04.88 Session Ended @16:54:06.24 -> slot {20 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 21 => { Type: :microSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr23aug0515h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0513h.spr.bz2}, 23 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0511h.spr.bz2}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0508h.spr.bz2}, 25 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0506h.spr.bz2}, 26 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0504h.spr.bz2}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0500h.spr.bz2"}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0422h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0420h.spr.bz2"}} @16:54:53.29 -> slot [19] =>[:microSPR_phyto, :standard, {lysed:1000, lysed2:1000}] {19 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @16:54:59.12 -> slot {[20,19] => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 21 => { Type: :microSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr23aug0515h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0513h.spr.bz2}, 23 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0511h.spr.bz2}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0508h.spr.bz2}, 25 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0506h.spr.bz2}, 26 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23aug0504h.spr.bz2}, 27 => { Type: :microSPR_phyto, State: :incompatible, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 28 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0500h.spr.bz2"}, 29 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0422h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug0420h.spr.bz2"}} @16:56:39.14 delay 10 seconds @16:56:49.17 SPR.command "start 2 0" @16:56:51.84 Waiting up to 11:00 for SPR to enter state 1 @16:58:49.50 SPR.command "hold_on" @16:58:50.89 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:58:51.64 Lysis2.seek 0uL @16:58:51.72 Lysis1.seek 0uL @16:59:12.85 delay 15 seconds @16:59:27.87 SPR.command "hold_off" @16:59:30.74 Waiting up to 38:00 for SPR to enter state 7 @17:25:21.35 SPR analysis completed @17:25:25.48 Ended log spr23aug0515h.spr.bz2 @17:25:25.51 SPR.command "stats_off" @17:25:26.24 Handoff.select :close @17:25:26.42
Gate.power :heat,:OFF @17:25:26.45 Gate.power :cartridge,:OFF @17:25:26.57 PinPort.in :unlocking @17:25:26.66 PinPort.seek :clear @17:25:28.60 Carousel.in :default @17:25:28.76 Carousel.home.seek 21 @17:25:29.12 Plunge.seek :locked @17:25:29.83 Archive.in :unlocking @17:25:30.01 Archive.seek :clear @17:25:30.11 Air.in :unlocking @17:25:30.31 Air.seek :clear @17:25:30.37 Lysis1.in :unlocking @17:25:30.62 Lysis1.seek :clear @17:25:30.73 Lysis2.in :unlocking @17:25:30.86 Lysis2.seek :clear @17:25:35.51
Gate.power :motor,:OFF @17:25:35.53 Gate.power :core,:OFF @17:25:35.57 Gate.power :analytic1,:OFF @17:25:35.60 Gate.power :analytic2,:OFF @17:25:35.63 Gate.power :main,:OFF @17:25:35.66 Safely stopped and ready to power off @17:25:36.19 Gate.power :sampler,:OFF,limit to 3500mA @17:25:39.37 Gate.power :main,:ON,limit to 5000mA @17:25:39.42 Gate.power :analytic1,:ON,limit to 5000mA @17:25:39.48 Gate.power :analytic2,:ON,limit to 5000mA @17:25:39.57 Powering core bus @17:25:41.93 SPR Module 1, FW Rev. B, FPGA Rev. C @17:25:42.35
Archive.configure :default @17:25:42.47 Air.configure :default @17:25:42.56 Lysis1.configure :default @17:25:42.66 Lysis2.configure :default @17:25:42.78 Plunge.configure :default @17:25:42.90 SamplePump.configure :default @17:25:43.02 Toroid.configure :default @17:25:43.15 Carousel.configure :default @17:25:43.27 Pressure.configure :default @17:25:43.40 ExtraPump.configure :default @17:25:43.50 PinPort.configure :default @17:25:43.60 All elf controllers running firmware version 4.84 @17:25:43.63 Gate.power :motor,:ON,limit to 5000mA @17:25:43.97 Archive.home.jog 0 @17:25:44.67 PinPort.home.jog 0 @17:25:44.72 Toroid.home.jog 0 @17:25:44.78 Plunge.home.jog 0 @17:25:44.82 Lysis1.home.jog 0 @17:25:44.86 Air.home.jog 0 @17:25:44.91 Carousel.home.jog 0 @17:25:44.96 Lysis2.home.jog 0 @17:25:45.92 Archive.in :homing @17:25:46.15 Archive.jog 1382 @17:25:46.23 Toroid.in :homing @17:25:46.29 Lysis2.in :homing @17:25:46.33 Air.in :homing @17:25:46.38 PinPort.in :homing @17:25:46.45 Plunge.in :homing @17:25:46.52 Lysis1.in :homing @17:25:47.44 Lysis2.jog 1390 @17:25:47.51 Air.jog 1365 @17:25:47.57 Toroid.jog 586 @17:25:47.60 PinPort.jog -111 @17:25:47.65 Plunge.jog 1553 @17:25:47.74 Lysis1.jog 1384 @17:25:48.16 Archive.setRawPosition! 50 @17:25:48.24 Archive.seek :home @17:25:48.36 PinPort.setRawPosition! 0 @17:25:48.44 PinPort.seek :home @17:25:48.57 PinPort.in :default @17:25:48.60 Archive.in :default @17:25:48.89 Lysis2.setRawPosition! 50 @17:25:48.98 Lysis2.seek :home @17:25:49.10 Air.setRawPosition! 50 @17:25:49.18 Air.seek :home @17:25:49.34 Plunge.setRawPosition! 50 @17:25:49.38 Lysis2.in :default @17:25:49.46 Lysis1.setRawPosition! 50 @17:25:49.62 Plunge.seek :home @17:25:49.69 Lysis1.seek :home @17:25:49.94 Air.in :default @17:25:49.97 Toroid.setRawPosition! 0 @17:25:50.15 Plunge.in :default @17:25:50.21 Lysis1.in :default @17:25:50.27 Toroid.seek :home @17:25:50.51 Toroid.in :default @17:25:50.73
Toroid.seek :clear @17:25:52.38 Carousel.in :default @17:25:52.55 Carousel.home.seek 21 @17:25:52.85 Selecting Cartridge 20 @17:25:53.19 Carousel.seek 20 @17:25:53.76 Lysis1.in :fast @17:25:53.85 Lysis1.seek :locked @17:25:56.50 Plunge.seek :locked @17:25:56.69 Air.in :fast @17:25:56.79 Air.seek :locked @17:25:58.38
Carousel.reconfigure :locked @17:25:58.57 Powering cartridge bus @17:26:01.25 Gate.setIR 1750,5000,0,6 @17:26:01.37 Gate.setIR 0,5000,0,6 @17:26:01.49 PuckHeater.configure :default @17:26:01.58 Gate.power :heat,:ON,limit to 5000mA @17:26:02.19 Mounted lysis Cartridge @17:26:02.22 WARNING: Using default puck temperature sensor calibration @17:26:02.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:26:04.37 SPR.command "read_sequences" @17:26:04.48 SPR.command "stats_on" @17:26:04.68 SPR.command "start 0 1" @17:26:05.37 SPR.start /var/log/esp/spr23aug0517h.spr.bz2 @17:26:07.34 Waiting for baseline SPR stabilization @17:26:07.35 Handoff.select :waste @17:26:14.15 Syringe tare is 19% of full scale @17:26:14.20 PinPort.in :locking @17:26:14.30 PinPort.seek :locked @17:26:16.86 Archive.in :fast @17:26:16.99 Lysis2.in :fast @17:26:17.08 Lysis1.seek 1000uL @17:26:17.10 Archive.seek :full @17:26:17.36 Lysis2.seek 1000uL @17:26:21.96 delayUntil "17:36:06.90" @17:36:06.91 Lysis2.in :DAmix @17:36:07.07 Lysis1.in :DAmix @17:36:07.68 Lysis2.seek 750uL @17:36:07.75 Lysis1.seek 750uL @17:36:42.89 delay 5 seconds @17:36:47.91 Handoff.select SPR @17:36:48.06 SPR.command "start 2 14" @17:36:48.60 SPR stabilized @17:36:48.80 delay 10 seconds @17:36:58.82 SPR.command "start 2 0" @17:37:01.97 Waiting up to 11:00 for SPR to enter state 1 @17:38:59.64 SPR.command "hold_on" @17:39:01.03 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:39:01.71 Lysis2.seek 450uL @17:39:01.81 Lysis1.seek 450uL @17:39:43.85 delay 15 seconds @17:39:58.88 SPR.command "hold_off" @17:40:01.68 Waiting up to 38:00 for SPR to enter state 7 @17:54:21.86 SPRsummary:10.8RIU,20.4RIU,97RIU,143RIU after 1791s @18:05:52.44 delay 10 seconds @18:06:02.47 SPR.command "start 2 0" @18:06:05.14 Waiting up to 11:00 for SPR to enter state 1 @18:08:02.81 SPR.command "hold_on" @18:08:04.19 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:08:04.82 Lysis2.seek 300uL @18:08:04.88 Lysis1.seek 300uL @18:08:26.04 delay 15 seconds @18:08:41.06 SPR.command "hold_off" @18:08:44.04 Waiting up to 38:00 for SPR to enter state 7 @18:34:34.80 delay 10 seconds @18:34:44.82 SPR.command "start 2 0" @18:34:48.02 Waiting up to 11:00 for SPR to enter state 1 @18:36:46.12 SPR.command "hold_on" @18:36:47.50 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:36:48.12 Lysis2.seek 150uL @18:36:48.16 Lysis1.seek 150uL @18:37:09.30 delay 15 seconds @18:37:24.32 SPR.command "hold_off" @18:37:27.34 Waiting up to 38:00 for SPR to enter state 7 @19:03:18.10 delay 10 seconds @19:03:28.12 SPR.command "start 2 0" @19:03:30.80 Waiting up to 11:00 for SPR to enter state 1 @19:05:28.48 SPR.command "hold_on" @19:05:29.86 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:05:30.94 Lysis2.seek 0uL @19:05:30.98 Lysis1.seek 0uL @19:05:52.13 delay 15 seconds @19:06:07.17 SPR.command "hold_off" @19:06:09.70 Waiting up to 38:00 for SPR to enter state 7 @19:25:08.29 Session Begun @19:25:11.02 -> showlog @19:26:01.42 -> exit @19:26:01.47 Session Ended @19:32:00.31 SPR analysis completed @19:32:04.43 Ended log spr23aug0517h.spr.bz2 @19:32:04.46 SPR.command "stats_off" @19:32:05.22 Handoff.select :close @19:32:05.44
Gate.power :heat,:OFF @19:32:05.93 Gate.power :cartridge,:OFF @19:32:06.05 PinPort.in :unlocking @19:32:06.14 PinPort.seek :clear @19:32:08.09 Carousel.in :default @19:32:08.25 Carousel.home.seek 20 @19:32:08.61 Plunge.seek :locked @19:32:08.82 Archive.in :unlocking @19:32:09.01 Archive.seek :clear @19:32:09.15 Air.in :unlocking @19:32:09.27 Air.seek :clear @19:32:09.46 Lysis1.in :unlocking @19:32:09.65 Lysis1.seek :clear @19:32:09.71 Lysis2.in :unlocking @19:32:09.88 Lysis2.seek :clear @19:32:14.50
Gate.power :motor,:OFF @19:32:14.52 Gate.power :core,:OFF @19:32:14.54 Gate.power :analytic1,:OFF @19:32:14.59 Gate.power :analytic2,:OFF @19:32:14.62 Gate.power :main,:OFF @19:32:14.65 Safely stopped and ready to power off @19:32:15.18 Gate.power :sampler,:OFF,limit to 3500mA @19:32:18.35 Gate.power :main,:ON,limit to 5000mA @19:32:18.37 Gate.power :analytic1,:ON,limit to 5000mA @19:32:18.42 Gate.power :analytic2,:ON,limit to 5000mA @19:32:18.52 Powering core bus @19:32:20.86 SPR Module 1, FW Rev. B, FPGA Rev. C @19:32:21.30
Archive.configure :default @19:32:21.46 Air.configure :default @19:32:21.56 Lysis1.configure :default @19:32:21.65 Lysis2.configure :default @19:32:21.77 Plunge.configure :default @19:32:21.88 SamplePump.configure :default @19:32:22.00 Toroid.configure :default @19:32:22.12 Carousel.configure :default @19:32:22.24 Pressure.configure :default @19:32:22.36 ExtraPump.configure :default @19:32:22.45 PinPort.configure :default @19:32:22.55 All elf controllers running firmware version 4.84 @19:32:22.57 Gate.power :motor,:ON,limit to 5000mA @19:32:23.03 Air.home.jog 0 @19:32:23.09 Archive.home.jog 0 @19:32:23.80 Carousel.home.jog 0 @19:32:23.91 Toroid.home.jog 0 @19:32:23.94 Lysis2.home.jog 0 @19:32:23.99 Plunge.home.jog 0 @19:32:24.04 Lysis1.home.jog 0 @19:32:24.08 PinPort.home.jog 0 @19:32:25.38 Archive.in :homing @19:32:25.53 Air.in :homing @19:32:25.68 Archive.jog 1380 @19:32:25.70 Air.jog 1359 @19:32:26.02 Toroid.in :homing @19:32:26.08 Plunge.in :homing @19:32:26.12 Lysis1.in :homing @19:32:26.20 Lysis2.in :homing @19:32:26.27 PinPort.in :homing @19:32:26.47 Plunge.jog 1556 @19:32:26.62 PinPort.jog -111 @19:32:26.65 Toroid.jog 589 @19:32:26.73 Lysis1.jog 1387 @19:32:26.79 Lysis2.jog 1393 @19:32:27.14 Air.setRawPosition! 50 @19:32:27.18 Archive.setRawPosition! 50 @19:32:27.22 PinPort.setRawPosition! 0 @19:32:27.40 Air.seek :home @19:32:27.48 PinPort.seek :home @19:32:27.53 Archive.seek :home @19:32:27.82 PinPort.in :default @19:32:27.97 Plunge.setRawPosition! 50 @19:32:28.02 Air.in :default @19:32:28.05 Archive.in :default @19:32:28.23 Plunge.seek :home @19:32:28.42 Lysis2.setRawPosition! 50 @19:32:28.45 Lysis1.setRawPosition! 50 @19:32:28.59 Lysis1.seek :home @19:32:28.62 Lysis2.seek :home @19:32:28.84 Plunge.in :default @19:32:29.00 Toroid.setRawPosition! 0 @19:32:29.03 Lysis1.in :default @19:32:29.16 Toroid.seek :home @19:32:29.31 Lysis2.in :default @19:32:29.34 Toroid.in :default @19:32:29.80
Toroid.seek :clear @19:32:31.46 Carousel.in :default @19:32:31.62 Carousel.home.seek 20 @19:32:31.91 Selecting Cartridge 19 @19:32:32.26 Carousel.seek 19 @19:32:32.83 Lysis1.in :fast @19:32:32.92 Lysis1.seek :locked @19:32:35.56 Plunge.seek :locked @19:32:35.76 Air.in :fast @19:32:35.85 Air.seek :locked @19:32:37.96
Carousel.reconfigure :locked @19:32:38.15 Powering cartridge bus @19:32:40.80 Gate.setIR 1750,5000,0,6 @19:32:40.93 Gate.setIR 0,5000,0,6 @19:32:41.02 PuckHeater.configure :default @19:32:41.11 Gate.power :heat,:ON,limit to 5000mA @19:32:41.74 Mounted lysis Cartridge @19:32:41.77 WARNING: Using default puck temperature sensor calibration @19:32:41.82 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:32:43.43 SPR.command "read_sequences" @19:32:43.51 SPR.command "stats_on" @19:32:43.69 SPR.command "start 0 1" @19:32:44.38 SPR.start /var/log/esp/spr23aug0519h.spr.bz2 @19:32:46.28 Waiting for baseline SPR stabilization @19:32:46.30 Handoff.select :waste @19:32:53.05 Syringe tare is 19% of full scale @19:32:53.10 PinPort.in :locking @19:32:53.19 PinPort.seek :locked @19:32:55.76 Archive.in :fast @19:32:55.81 Lysis2.in :fast @19:32:55.89 Lysis1.seek 1000uL @19:32:56.16 Lysis2.seek 1000uL @19:32:56.18 Archive.seek :full @19:33:00.77 delayUntil "19:42:45.84" @19:42:45.86 Lysis2.in :DAmix @19:42:46.01 Lysis1.in :DAmix @19:42:46.61 Lysis2.seek 750uL @19:42:46.72 Lysis1.seek 750uL @19:43:21.81 delay 5 seconds @19:43:26.83 Handoff.select SPR @19:43:26.99 SPR.command "start 2 14" @19:43:27.55 SPR stabilized @19:43:27.74 delay 10 seconds @19:43:37.76 SPR.command "start 2 0" @19:43:40.92 Waiting up to 11:00 for SPR to enter state 1 @19:45:38.59 SPR.command "hold_on" @19:45:39.97 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:45:40.62 Lysis2.seek 450uL @19:45:40.67 Lysis1.seek 450uL @19:46:22.75 delay 15 seconds @19:46:37.77 SPR.command "hold_off" @19:46:40.62 Waiting up to 38:00 for SPR to enter state 7 @20:01:00.80 SPRsummary:12.7RIU,21.7RIU,88RIU,127RIU after 1791s @20:12:31.39 delay 10 seconds @20:12:41.43 SPR.command "start 2 0" @20:12:44.61 Waiting up to 11:00 for SPR to enter state 1 @20:14:42.69 SPR.command "hold_on" @20:14:44.08 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:14:44.72 Lysis2.seek 300uL @20:14:44.81 Lysis1.seek 300uL @20:15:05.92 delay 15 seconds @20:15:20.94 SPR.command "hold_off" @20:15:23.93 Waiting up to 38:00 for SPR to enter state 7 @20:41:14.69 delay 10 seconds @20:41:24.71 SPR.command "start 2 0" @20:41:27.40 Waiting up to 11:00 for SPR to enter state 1 @20:43:25.05 SPR.command "hold_on" @20:43:26.44 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:43:27.15 Lysis2.seek 150uL @20:43:27.25 Lysis1.seek 150uL @20:43:48.35 delay 15 seconds @20:44:03.37 SPR.command "hold_off" @20:44:06.81 Waiting up to 38:00 for SPR to enter state 7 @21:09:57.99 delay 10 seconds @21:10:08.01 SPR.command "start 2 0" @21:10:10.69 Waiting up to 11:00 for SPR to enter state 1 @21:12:08.36 SPR.command "hold_on" @21:12:10.27 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:12:10.98 Lysis2.seek 0uL @21:12:11.02 Lysis1.seek 0uL @21:12:32.21 delay 15 seconds @21:12:47.24 SPR.command "hold_off" @21:12:50.54 Waiting up to 38:00 for SPR to enter state 7 @21:38:40.20 SPR analysis completed @21:38:44.51 Ended log spr23aug0519h.spr.bz2 @21:38:44.53 SPR.command "stats_off" @21:38:45.96 Handoff.select :close @21:38:46.13
Gate.power :heat,:OFF @21:38:46.15 Gate.power :cartridge,:OFF @21:38:46.28 PinPort.in :unlocking @21:38:46.36 PinPort.seek :clear @21:38:48.30 Carousel.in :default @21:38:48.46 Carousel.home.seek 19 @21:38:49.29 Plunge.seek :locked @21:38:49.53 Archive.in :unlocking @21:38:49.72 Archive.seek :clear @21:38:49.86 Air.in :unlocking @21:38:50.04 Air.seek :clear @21:38:50.19 Lysis1.in :unlocking @21:38:50.37 Lysis1.seek :clear @21:38:50.44 Lysis2.in :unlocking @21:38:50.60 Lysis2.seek :clear @21:38:55.23
Gate.power :motor,:OFF @21:38:55.25 Gate.power :core,:OFF @21:38:55.29 Gate.power :analytic1,:OFF @21:38:55.32 Gate.power :analytic2,:OFF @21:38:55.36 Gate.power :main,:OFF @21:38:55.38 Safely stopped and ready to power off @21:38:55.92 Cartridge::None in MAIN -- Not enough standard cartridges remain @21:38:56.00 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @10:50:41.69PDT06-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @10:50:43.26 >BREAK< ESP3 Gateway boot v1.43 for core bus @10:50:43.29 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @10:50:46.55 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @10:50:47.13 Sea pressure (Abs) tare is 6% of full scale @10:50:51.64 Polling Can every 2:00 @10:50:51.69 Waiting for espclients... @10:50:52.14 Session Begun @10:50:56.79 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry}} @10:57:01.66 -> require 'vacuumCart' true @10:57:11.44 -> vacToroid @10:57:11.49 Vacuuming protocol for dry cartridges @10:57:11.54 Gate.power :sampler,:OFF,limit to 3500mA @10:57:11.56 Gate.power :main,:ON,limit to 5000mA @10:57:11.60 Gate.power :analytic1,:ON,limit to 5000mA @10:57:11.67 Gate.power :analytic2,:ON,limit to 5000mA @10:57:11.85 Powering core bus @10:57:14.10 SPR Module 1, FW Rev. B, FPGA Rev. C @10:57:14.61 Archive.configure :default @10:57:14.72 Air.configure :default @10:57:14.81 Lysis1.configure :default @10:57:14.91 Lysis2.configure :default @10:57:15.03 Plunge.configure :default @10:57:15.14 SamplePump.configure :default @10:57:15.27 Toroid.configure :default @10:57:15.39 Carousel.configure :default @10:57:15.51 Pressure.configure :default @10:57:15.66 ExtraPump.configure :default @10:57:15.76 PinPort.configure :default @10:57:15.84 All elf controllers running firmware version 4.84 @10:57:15.89 Gate.power :motor,:ON,limit to 5000mA @10:57:16.51 Archive.home.jog 0 @10:57:16.65 Air.home.jog 0 @10:57:16.71 Lysis1.home.jog 0 @10:57:16.85 Lysis2.home.jog 0 @10:57:17.00 Plunge.home.jog 0 @10:57:17.12 Toroid.home.jog 0 @10:57:17.23 Carousel.home.jog 0 @10:57:17.44 PinPort.home.jog 0 @10:57:17.91 Archive.in :homing @10:57:18.72 Archive.jog 1382 @10:57:19.01 Toroid.in :homing @10:57:19.07 Plunge.in :homing @10:57:19.13 Lysis1.in :homing @10:57:19.22 Air.in :homing @10:57:19.29 Lysis2.in :homing @10:57:19.56 Plunge.jog 400 @10:57:19.61 Lysis1.jog 1385 @10:57:19.68 Toroid.jog 615 @10:57:19.72 Lysis2.jog 1390 @10:57:19.78 Air.jog 157 @10:57:20.13 Archive.setRawPosition! 50 @10:57:20.16 PinPort.in :homing @10:57:20.29 Archive.seek :home @10:57:20.33 PinPort.jog -108 @10:57:20.55 Air.setRawPosition! 50 @10:57:20.58 Plunge.setRawPosition! 50 @10:57:20.74 Air.seek :home @10:57:20.77 Plunge.seek :home @10:57:20.96 PinPort.setRawPosition! 0 @10:57:20.99 Archive.in :default @10:57:21.18 PinPort.seek :home @10:57:21.33 Plunge.in :default @10:57:21.38 Lysis2.setRawPosition! 50 @10:57:21.42 Air.in :default @10:57:21.48 Lysis1.setRawPosition! 50 @10:57:21.54 PinPort.in :default @10:57:21.82 Lysis2.seek :home @10:57:21.88 Lysis1.seek :home @10:57:22.08 Toroid.setRawPosition! 0 @10:57:22.16 Toroid.seek :home @10:57:22.32 Toroid.in :default @10:57:22.37 Lysis2.in :default @10:57:22.40 Lysis1.in :default @10:57:22.88 Toroid.seek :clear @10:57:24.52 Carousel.in :default @10:57:24.68 Carousel.home.seek 1 @10:57:25.12 Waiting for bill to answer "Disconnect the intake line. push return" @10:57:29.27 Answered Disconnect the intake line. push return"" @10:57:30.14 Toroid.seek :clear @10:57:30.24 Carousel.in :default @10:57:30.39 Carousel.home.seek 1 @10:57:30.67 SamplePump.setPosition! 0ml @10:57:30.74 Intake.open @10:57:31.42 Exhaust.open @10:57:32.17 SamplePump.seek 50ml @10:58:33.31 Exhaust.close @10:58:34.03 Bypass.open @10:58:34.75 SamplePump.seek 75ml @10:59:05.53 Intake.close @10:59:06.24 Bypass.close @10:59:06.93 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @10:59:59.28 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @10:59:59.30 Exhaust.open @11:00:00.07 Bypass.open @11:00:00.83 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @11:00:26.21 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @11:00:26.23 delay 30 seconds @11:00:56.28 Clearing fluid from cartridge-side of Toroid @11:00:56.61 Carousel.seek 60 @11:01:08.86 Toroid.in :hold @11:01:09.36 Toroid.seek :cartridge @11:01:11.33 Toroid.in :default @11:01:11.42 Toroid.seek :clear @11:01:13.26 delay 2 seconds @11:01:15.37 Toroid.in :hold @11:01:15.47 Toroid.seek :bypass @11:01:17.14 Toroid.in :default @11:01:17.23 Toroid.seek :clear @11:01:18.88 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @11:01:41.57 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @11:01:42.39 Toroid.seek :clear @11:01:42.49 Carousel.in :default @11:01:42.65 Carousel.home.seek 60 @11:01:42.94 Waiting for bill to answer "Turn the pump on. Push Return" @11:01:46.49 Answered Turn the pump on. Push Return"" @11:01:46.51 Vacuuming individual cartridges. @11:01:47.40 Toroid.seek :clear @11:01:47.51 Carousel.in :default @11:01:47.70 Carousel.home.seek 60 @11:01:48.28 Carousel.seek 60 @11:01:48.57 Lysis1.in :fast @11:01:48.66 Lysis1.seek :locked @11:01:51.32 Plunge.seek :locked @11:01:51.52 Air.in :fast @11:01:51.63 Air.seek :locked @11:01:53.25 Carousel.reconfigure :locked @11:01:53.44 Powering cartridge bus @11:01:56.10 Gate.setIR 1750,5000,0,6 @11:01:56.23 Gate.setIR 0,5000,0,6 @11:01:56.32 PuckHeater.configure :default @11:01:56.42 Gate.power :heat,:ON,limit to 5000mA @11:01:57.18 Mounted lysis Cartridge board#Q05041 @11:01:57.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:01:57.28 Vacuuming Vent Top cartridge 60 for 30 seconds @11:01:57.32 Air.in :default @11:01:57.42 Air.seek :evac @11:02:10.50 Toroid.in :hold @11:02:10.59 Toroid.seek :cartridge @11:02:12.45 Toroid.in :default @11:02:12.54 Toroid.seek :clear @11:02:14.38 Polling Can every 2:00 @11:02:14.93 PuckHeater.configure :default #ambient 22.8C @11:02:15.02 PuckHeater.seek 41C,1:00 @11:02:37.06 PuckHeater.hold 3 seconds,41C @11:02:40.22 delayUntil "11:02:43.61" @11:02:43.73 Toroid.in :hold @11:02:43.82 Toroid.seek :bypass @11:02:45.48 Toroid.in :default @11:02:45.58 Toroid.seek :clear @11:02:47.24 delay 2 seconds @11:02:49.27 Air.seek :full @11:03:03.77 Gate.power :heat,:OFF @11:03:03.80 Gate.power :cartridge,:OFF @11:03:03.92 Carousel.in :default @11:03:04.07 Carousel.home.seek 60 @11:03:04.51 Air.in :unlocking @11:03:04.65 Air.seek :clear @11:03:05.72 Lysis1.in :unlocking @11:03:05.92 Plunge.in :unlocking @11:03:05.96 Lysis1.seek :clear @11:03:06.12 Plunge.seek :clear @11:03:07.37 Cartridges under vacuum. Individual Leak Test begins @11:03:07.40 Waiting for bill to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @11:03:23.77 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @11:03:23.79 delay 10:00 @11:13:23.85 Waiting for bill to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @11:13:28.98 Session Begun @11:13:52.03 -> Sampler::PumpPrimeDelay -3:30 @11:14:15.15 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @11:14:15.17 move through each cartridge position & record end vacuum @11:14:15.22 Vacuuming cartridge 60 @11:14:15.60 Carousel.seek 60 @11:14:15.94 Lysis1.in :fast @11:14:16.05 Lysis1.seek :locked @11:14:18.72 Plunge.in :default @11:14:18.87 Plunge.seek :locked @11:14:19.06 Air.in :fast @11:14:19.16 Air.seek :locked @11:14:20.78 Carousel.reconfigure :locked @11:14:21.00 Powering cartridge bus @11:14:23.69 Gate.setIR 1750,5000,0,6 @11:14:23.84 Gate.setIR 0,5000,0,6 @11:14:23.94 PuckHeater.configure :default #ambient 22.8C @11:14:24.04 Gate.power :heat,:ON,limit to 5000mA @11:14:27.26 Mounted lysis Cartridge board#Q05041 @11:14:27.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:14:27.44 -> exit @11:14:27.53 Session Ended @11:14:27.69 Toroid.in :hold @11:14:27.79 Toroid.seek :cartridge @11:14:29.68 Toroid.in :default @11:14:29.78 Toroid.seek :clear @11:14:31.64 Waiting for bill to answer "record Vacuum" @11:15:32.46 Answered record Vacuum"" @11:15:32.60 Toroid.in :hold @11:15:32.69 Toroid.seek :bypass @11:15:34.35 Toroid.in :default @11:15:34.44 Toroid.seek :clear @11:15:36.09 Waiting for bill to answer "recharge if needed" @11:15:42.44 Answered recharge if needed"" @11:15:42.48 Gate.power :heat,:OFF @11:15:42.50 Gate.power :cartridge,:OFF @11:15:42.68 Carousel.in :default @11:15:42.83 Carousel.home.seek 60 @11:15:43.27 Air.in :unlocking @11:15:43.44 Air.seek :clear @11:15:44.89 Lysis1.in :unlocking @11:15:45.07 Lysis1.seek :clear @11:15:45.16 Plunge.in :unlocking @11:15:45.29 Plunge.seek :clear @11:15:46.48 Waiting for bill to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @11:15:56.88 UserAbort in bill -- User Abort!! @11:17:10.15 -> refillSampleLines @11:17:10.18 Refilling sample & bypass lines with fluid. @11:17:11.00 Toroid.seek :clear @11:17:11.11 Carousel.in :default @11:17:11.26 Carousel.home.seek 60 @11:17:11.55 Waiting for bill to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @11:17:15.88 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @11:17:15.90 Intake.open @11:17:16.62 SamplePump.setPosition! 0ml @11:17:16.76 SamplePump.seek 30ml @11:17:53.63 Bypass.close @11:17:54.43 SamplePump.seek 55ml @11:18:25.22 delay 1 second @11:18:26.25 Intake.close @11:18:26.34 Exhaust.close @11:18:27.12 Sampling Loop filled with water @11:21:13.76 -> fire :microSPR_phyto @11:21:14.58 Toroid.seek :clear @11:21:14.69 Carousel.in :default @11:21:14.84 Carousel.home.seek 60 @11:21:15.15 Selecting Cartridge 60 @11:21:16.10 Carousel.seek 60 @11:21:16.39 Lysis1.in :fast @11:21:16.49 Lysis1.seek :locked @11:21:19.16 Plunge.in :default @11:21:19.30 Plunge.seek :locked @11:21:19.44 Air.in :fast @11:21:19.53 Air.seek :locked @11:21:21.13 Carousel.reconfigure :locked @11:21:21.32 Powering cartridge bus @11:21:23.99 Gate.setIR 1750,5000,0,6 @11:21:24.13 Gate.setIR 0,5000,0,6 @11:21:24.23 PuckHeater.configure :default #ambient 22.8C @11:21:24.32 Gate.power :heat,:ON,limit to 5000mA @11:21:25.02 Mounted lysis Cartridge board#Q05041 @11:21:25.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:21:25.44 Preparing to filter cartridge 60 @11:21:25.75 Gate.power :sampler,:ON @11:21:25.88 Priming sample loop w/150ml, bypass w/50ml @11:21:25.90 SamplePump.setPosition! 0ml @11:21:25.98 Bypass.open @11:21:27.50 Intake.open @11:21:28.25 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:21:30.84 Vacuum tare is 56% of full scale @11:21:31.02 Pressure.in :default @11:21:31.11 Pressure.hold @11:21:31.37 Waiting for external sampler to finish priming... @11:21:31.39 delayUntil "11:24:55.77" @11:24:55.82 SamplePump.in :prime @11:24:55.92 SamplePump.seek 50ml,2:53 @11:25:56.94 Pressure.stop @11:25:57.02 SamplePump.setPosition! 0ml @11:25:57.09 Exhaust.open @11:25:58.56 Bypass.close @11:26:00.11 Pressure.in :default @11:26:00.20 Pressure.hold @11:26:00.52 SamplePump.in :prime @11:26:00.63 SamplePump.seek 150ml,7:39 @11:29:03.69 Pressure.stop @11:29:03.80 Bypass.open @11:29:04.57 Intake.close @11:29:05.36 Toroid.in :hold @11:29:05.45 Toroid.seek :crack @11:29:07.02 Toroid.seek :cartridge @11:29:08.16 Toroid.in :default @11:29:08.25 Toroid.seek :clear @11:29:10.29 Pressurizing cartridge to 26psi above 16.5psia @11:29:10.31 SamplePump.setPosition! 0ml @11:29:10.40 Intake.open @11:29:11.08 Bypass.close @11:29:11.80 Exhaust.close @11:29:12.66 Pressure.in :default @11:29:12.75 Pressure.hold @11:29:13.01 SamplePump.in :charge @11:29:13.10 SamplePump.seek 2.3ml,42 seconds @11:29:28.01 Pressure.stop @11:29:28.11 Intake.close @11:29:28.86 Pressurized cartridge with 1.004ml to 26.1psi @11:29:28.88 Isolated @35.3psia (18.7psi) @11:29:28.92 Verifying cartridge holds at least 0.65..0.85 of 35.3psia @11:29:28.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:29:33.58 Cartridge pressure fell 1% to 34.8psia @11:29:33.75 Sampling 50.0ml @11:29:33.77 SamplePump.setPosition! 0ml @11:29:33.86 Exhaust.open @11:29:34.61 Intake.open @11:29:35.42 Pressure.in :default @11:29:35.51 Pressure.hold @11:29:35.72 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:29:35.94 SamplePump.in :sample @11:29:36.04 SamplePump.seek 50ml,4:41 @11:29:39.06 Cartridge 60 filtering 1/50ml at 0.55ml/s with 26.4psi vacuum @11:30:39.07 Cartridge 60 filtering 29/50ml at 0.47ml/s with 19.7psi vacuum 2.1m deep (17.8psia) @11:31:31.81 Pressure.stop @11:31:31.99 Intake.close @11:31:32.05 Exhaust.close @11:31:32.30 Toroid.in :hold @11:31:32.39 Toroid.seek :bypass @11:31:34.05 Toroid.in :default @11:31:34.65 Toroid.seek :clear @11:31:36.39 Sampled 50.0ml @11:31:38.13 SPR.command "read_sequences" @11:31:38.73 SPR.command "stats_on" @11:31:38.91 SPR.command "start 0 1" @11:31:39.55 Gate.power :sampler,:OFF @11:31:39.62 SPR.start /var/log/esp/spr23aug0611h.spr.bz2 @11:31:41.59 Handoff.select :waste @11:31:48.35 Syringe tare is 18% of full scale @11:31:48.39 PinPort.in :locking @11:31:48.50 PinPort.seek :locked @11:31:51.07 Archive.in :fast @11:31:51.18 Lysis2.in :fast @11:31:51.25 Lysis1.seek 1350uL @11:31:51.32 Archive.seek :full @11:31:51.52 Lysis2.seek 950uL @11:31:56.34 Plunge.seek :reagent @11:31:57.33 Evacuating puck with filter bubble point of 50psi @11:31:57.37 PinPort.seek :locked @11:31:57.52 Handoff.select :waste @11:32:04.14 Air syringe pumping up to 1 strokes to reach 8psi @11:32:04.16 Air stroke #1 of 1 @11:32:04.94 Air.in :pressurize @11:32:05.04 Air.seek :pressurized @11:32:10.82 0.3psi after pressurize @11:32:10.85 Air.in :compress @11:32:10.96 Air.seek :evac @11:32:14.01 0.6psi after compress @11:32:24.64 0.0psi after compress (stable) @11:32:24.67 Air.in :default @11:32:24.77 Air.hold @11:32:25.03 at 0.0psi Opened to waste @11:32:25.04 Handoff.select :blocked @11:32:25.23 Archive syringe pumping up to 8 strokes to reach 50psi @11:32:25.24 Archive stroke #1 of 8 @11:32:25.37 Archive.in :pressurize @11:32:25.46 Archive.seek :pressurized @11:32:38.02 11.2psi after pressurize @11:32:49.64 19.2psi after pressurize (stable) @11:32:49.68 Archive.in :compress @11:32:49.79 Archive.seek :empty @11:33:04.45 26.9psi after compress @11:33:15.05 27.0psi after compress (stable) @11:33:15.08 Archive.in :gulp @11:33:15.70 Archive.seek :topVent @11:33:22.13 26.5psi after gulp @11:33:47.24 7.3psi after gulp (stable) @11:33:47.26 Archive stroke #2 of 8 @11:33:47.39 Archive.in :pressurize @11:33:47.48 Archive.seek :pressurized @11:34:00.02 12.7psi after pressurize @11:34:08.64 16.9psi after pressurize (stable) @11:34:08.70 Archive.in :compress @11:34:08.80 Archive.seek :empty @11:34:23.46 22.1psi after compress @11:34:37.11 26.2psi after compress (stable) @11:34:37.14 Archive.in :gulp @11:34:37.26 Archive.seek :topVent @11:34:43.55 25.6psi after gulp @11:34:51.14 23.3psi after gulp (stable) @11:34:51.16 Archive stroke #3 of 8 @11:34:51.33 Archive.in :pressurize @11:34:51.42 Archive.seek :pressurized @11:35:03.96 25.2psi after pressurize @11:35:12.56 27.7psi after pressurize (stable) @11:35:12.59 Archive.in :compress @11:35:12.70 Archive.seek :empty @11:35:27.35 38.8psi after compress @11:35:37.95 40.5psi after compress (stable) @11:35:37.98 Archive.in :gulp @11:35:38.09 Archive.seek :topVent @11:35:44.38 39.9psi after gulp @11:36:32.98 2.4psi after gulp (stable) @11:36:32.99 Archive stroke #4 of 8 @11:36:33.13 Archive.in :pressurize @11:36:33.22 Archive.seek :pressurized @11:36:45.79 9.5psi after pressurize @11:36:55.39 14.2psi after pressurize (stable) @11:36:55.42 Archive.in :compress @11:36:55.53 Archive.seek :empty @11:37:10.77 21.5psi after compress @11:37:21.37 22.5psi after compress (stable) @11:37:21.40 Archive.in :gulp @11:37:21.51 Archive.seek :topVent @11:37:27.82 19.3psi after gulp @11:37:53.92 2.9psi after gulp (stable) @11:37:53.94 Archive stroke #5 of 8 @11:37:54.07 Archive.in :pressurize @11:37:54.16 Archive.seek :pressurized @11:38:06.68 9.7psi after pressurize @11:38:12.78 14.1psi after pressurize (stable) @11:38:12.81 Archive.in :compress @11:38:12.91 Archive.seek :empty @11:38:27.56 22.0psi after compress @11:38:38.16 22.4psi after compress (stable) @11:38:38.21 Archive.in :gulp @11:38:38.34 Archive.seek :topVent @11:38:44.62 20.0psi after gulp @11:39:02.22 2.3psi after gulp (stable) @11:39:02.24 Archive stroke #6 of 8 @11:39:02.37 Archive.in :pressurize @11:39:02.46 Archive.seek :pressurized @11:39:14.99 9.6psi after pressurize @11:39:22.08 13.6psi after pressurize (stable) @11:39:22.11 Archive.in :compress @11:39:22.23 Archive.seek :empty @11:39:36.93 19.8psi after compress @11:39:48.53 22.2psi after compress (stable) @11:39:48.56 Archive.in :gulp @11:39:48.66 Archive.seek :topVent @11:39:54.95 19.0psi after gulp @11:40:19.05 1.6psi after gulp (stable) @11:40:19.07 Archive stroke #7 of 8 @11:40:19.21 Archive.in :pressurize @11:40:19.30 Archive.seek :pressurized @11:40:31.84 8.7psi after pressurize @11:40:41.95 13.5psi after pressurize (stable) @11:40:41.97 Archive.in :compress @11:40:42.08 Archive.seek :empty @11:40:56.79 18.5psi after compress @11:41:08.89 21.9psi after compress (stable) @11:41:08.92 Archive.in :gulp @11:41:09.03 Archive.seek :topVent @11:41:15.36 17.2psi after gulp @11:41:39.96 1.8psi after gulp (stable) @11:41:39.97 Archive stroke #8 of 8 @11:41:40.11 Archive.in :pressurize @11:41:40.20 Archive.seek :pressurized @11:41:52.73 8.9psi after pressurize @11:42:02.33 13.3psi after pressurize (stable) @11:42:02.36 Archive.in :compress @11:42:02.46 Archive.seek :empty @11:42:17.16 19.5psi after compress @11:42:28.76 22.2psi after compress (stable) @11:42:28.78 Archive.in :default @11:42:28.89 Archive.hold @11:42:29.14 Handoff.select :blocked @11:42:29.21 Underpressure in PROCESSING -- Reached only 22.3 of 50.0psi after pumping 13840uL @11:42:29.23 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @11:42:57.19 Session Begun @11:43:02.00 Cmd::Error in bill -- Not Processing @11:43:02.05 UserAbort in bill -- User Abort!! @11:43:14.25 -> esp :esp @11:43:20.09 -> Cartridge.unloc @11:43:20.17 NoMethodError in bill -- undefined method `unloc' for Cartridge @11:43:23.17 -> Cartridge.unlock @11:43:23.25 Gate.power :heat,:OFF @11:43:23.26 Gate.power :cartridge,:OFF @11:43:23.43 PinPort.in :unlocking @11:43:23.54 PinPort.seek :clear @11:43:25.53 Carousel.in :default @11:43:25.78 Carousel.home.seek 60 @11:43:26.22 Archive.in :unlocking @11:43:26.43 Archive.seek :clear @11:43:26.52 Air.in :unlocking @11:43:26.80 Air.seek :clear @11:43:26.92 Lysis2.in :unlocking @11:43:27.14 Lysis2.seek :clear @11:43:31.20 Lysis1.in :unlocking @11:43:31.39 Lysis1.seek :clear @11:43:31.49 Plunge.in :unlocking @11:43:31.70 Plunge.seek :clear @11:45:11.25 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug0611h.spr.bz2}} @11:45:43.93 -> slot [60] =>[:dry, :microSPR_phyto] {60 => { Type: :microSPR_phyto, State: :dry}} @11:45:58.35 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry}} @11:46:35.10 -> exit @11:46:35.14 Thread::Aborted in PROCESSING -- "Aborted by bill" @11:46:35.28 Session Ended @11:46:35.35 Thread::Aborted in stopSPRafterError -- "Aborted by bill" @11:46:35.49 Thread::ChildDied in PROCESSING -- :stopSPRafterError @11:46:35.56 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @11:46:42.47 Thread::Aborted in SPRlogger -- "Aborted by bill" @11:46:42.53 Thread::ChildDied in PROCESSING -- :SPRlogger @11:46:42.60 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:47:27.91 -> MainThread.exit # @11:47:28.11 Session Ended @11:47:41.40PDT06-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @11:47:43.08 >BREAK< ESP3 Gateway boot v1.43 for core bus @11:47:43.11 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @11:47:46.54 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @11:47:47.15 Sea pressure (Abs) tare is 6% of full scale @11:47:51.77 Polling Can every 2:00 @11:47:51.82 Waiting for espclients... @11:49:00.05 Session Begun @11:49:10.72 -> fire :microSPR_phyto @11:49:10.93 Gate.power :sampler,:OFF,limit to 3500mA @11:49:10.95 Gate.power :analytic1,:ON,limit to 5000mA @11:49:11.00 Gate.power :analytic2,:ON,limit to 5000mA @11:49:11.59 SPR Module 1, FW Rev. B, FPGA Rev. C @11:49:11.88 Archive.configure :default @11:49:11.98 Archive.hold @11:49:12.08 Archive.configure :default @11:49:12.27 Air.configure :default @11:49:12.36 Air.hold @11:49:12.48 Air.configure :default @11:49:12.64 Lysis1.configure :default @11:49:12.74 Lysis1.hold @11:49:12.85 Lysis1.configure :default @11:49:13.02 Lysis2.configure :default @11:49:13.11 Lysis2.hold @11:49:13.22 Lysis2.configure :default @11:49:13.41 Plunge.configure :default @11:49:13.51 Plunge.hold @11:49:13.64 Plunge.configure :default @11:49:13.84 SamplePump.configure :default @11:49:14.04 Toroid.configure :default @11:49:14.14 Toroid.hold @11:49:14.24 Toroid.configure :default @11:49:14.43 Carousel.configure :default @11:49:14.53 Carousel.hold @11:49:15.06 Carousel.configure :default @11:49:15.29 Pressure.configure :default @11:49:15.49 ExtraPump.configure :default @11:49:15.68 PinPort.configure :default @11:49:15.79 PinPort.hold @11:49:15.90 PinPort.configure :default @11:49:15.99 All elf controllers running firmware version 4.84 @11:49:16.80 Toroid.seek :clear @11:49:16.91 Carousel.in :default @11:49:17.07 Carousel.home.seek 60 @11:49:17.38 Selecting Cartridge 60 @11:49:17.77 Carousel.seek 60 @11:49:18.06 Lysis1.in :fast @11:49:18.16 Lysis1.seek :locked @11:49:20.84 Plunge.seek :locked @11:49:21.04 Air.in :fast @11:49:21.13 Air.seek :locked @11:49:22.73 Carousel.reconfigure :locked @11:49:22.93 Powering cartridge bus @11:49:25.59 Gate.setIR 1750,5000,0,6 @11:49:25.74 Gate.setIR 0,5000,0,6 @11:49:25.84 PuckHeater.configure :default @11:49:25.93 Gate.power :heat,:ON,limit to 5000mA @11:49:26.69 Mounted lysis Cartridge board#Q05046 @11:49:26.75 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:49:27.12 Preparing to filter cartridge 60 @11:49:27.39 Gate.power :sampler,:ON @11:49:27.51 Priming sample loop w/150ml, bypass w/50ml @11:49:27.53 SamplePump.setPosition! 0ml @11:49:27.62 Exhaust.close @11:49:27.76 Bypass.open @11:49:29.30 Intake.open @11:49:30.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:49:32.69 Vacuum tare is 56% of full scale @11:49:33.37 Pressure.in :default @11:49:33.46 Pressure.hold @11:49:33.76 Waiting for external sampler to finish priming... @11:49:33.78 delayUntil "11:52:57.41" @11:52:57.46 SamplePump.in :prime @11:52:57.56 SamplePump.seek 50ml,2:53 @11:53:58.59 Pressure.stop @11:53:58.67 SamplePump.setPosition! 0ml @11:53:58.74 Exhaust.open @11:54:00.21 Bypass.close @11:54:01.79 Pressure.in :default @11:54:01.88 Pressure.hold @11:54:02.20 SamplePump.in :prime @11:54:02.31 SamplePump.seek 150ml,7:39 @11:57:05.08 Pressure.stop @11:57:05.19 Bypass.open @11:57:05.93 Intake.close @11:57:06.75 Toroid.in :hold @11:57:06.84 Toroid.seek :crack @11:57:08.42 Toroid.seek :cartridge @11:57:09.56 Toroid.in :default @11:57:09.69 Toroid.seek :clear @11:57:11.76 Pressurizing cartridge to 26psi above 16.5psia @11:57:11.78 SamplePump.setPosition! 0ml @11:57:11.89 Intake.open @11:57:12.61 Bypass.close @11:57:13.32 Exhaust.close @11:57:14.19 Pressure.in :default @11:57:14.28 Pressure.hold @11:57:14.55 SamplePump.in :charge @11:57:14.66 SamplePump.seek 2.3ml,42 seconds @11:57:29.42 Pressure.stop @11:57:29.51 Intake.close @11:57:30.30 Pressurized cartridge with 0.986ml to 26.1psi @11:57:30.32 Isolated @35.4psia (18.9psi) @11:57:30.36 Verifying cartridge holds at least 0.65..0.85 of 35.4psia @11:57:30.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:57:35.02 Cartridge pressure fell 1% to 35.1psia @11:57:35.15 Sampling 50.0ml @11:57:35.17 SamplePump.setPosition! 0ml @11:57:35.26 Exhaust.open @11:57:36.01 Intake.open @11:57:37.36 Pressure.in :default @11:57:37.46 Pressure.hold @11:57:37.65 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:57:37.88 SamplePump.in :sample @11:57:37.98 SamplePump.seek 50ml,4:41 @11:57:41.01 Cartridge 60 filtering 1/50ml at 0.59ml/s with 26.7psi vacuum @11:58:41.02 Cartridge 60 filtering 32/50ml at 0.51ml/s with 24.4psi vacuum 5.5m deep (22.8psia) @11:59:20.25 Pressure.stop @11:59:20.43 Intake.close @11:59:20.47 Exhaust.close @11:59:20.78 Toroid.in :hold @11:59:20.87 Toroid.seek :bypass @11:59:22.53 Toroid.in :default @11:59:22.63 Toroid.seek :clear @11:59:24.26 Sampled 50.0ml @11:59:25.99 SPR.command "read_sequences" @11:59:26.23 SPR.command "stats_on" @11:59:26.40 SPR.command "start 0 1" @11:59:27.11 SPR.start /var/log/esp/spr23aug0611h#2.spr.bz2 @11:59:27.42 Gate.power :sampler,:OFF @11:59:29.08 Handoff.select :waste @11:59:35.82 Syringe tare is 18% of full scale @11:59:35.86 PinPort.in :locking @11:59:35.97 PinPort.seek :locked @11:59:38.54 Archive.in :fast @11:59:38.60 Lysis2.in :fast @11:59:38.67 Lysis1.seek 1350uL @11:59:38.89 Archive.seek :full @11:59:38.93 Lysis2.seek 950uL @11:59:43.78 Plunge.seek :reagent @11:59:44.81 Evacuating puck with filter bubble point of 50psi @11:59:44.84 PinPort.seek :locked @11:59:44.97 Handoff.select :waste @11:59:51.60 Air syringe pumping up to 1 strokes to reach 8psi @11:59:51.63 Air stroke #1 of 1 @11:59:51.78 Air.in :pressurize @11:59:51.87 Air.seek :pressurized @11:59:57.68 5.4psi after pressurize @11:59:57.71 Air.in :compress @11:59:57.81 Air.seek :evac @12:00:00.90 2.8psi after compress @12:00:12.00 0.9psi after compress (stable) @12:00:12.02 Air.in :default @12:00:12.14 Air.hold @12:00:12.42 at 0.8psi Opened to waste @12:00:12.44 Handoff.select :blocked @12:00:12.61 Archive syringe pumping up to 8 strokes to reach 50psi @12:00:12.63 Archive stroke #1 of 8 @12:00:12.75 Archive.in :pressurize @12:00:13.39 Archive.seek :pressurized @12:00:25.92 1.6psi after pressurize @12:00:31.52 1.6psi after pressurize (stable) @12:00:31.55 Archive.in :compress @12:00:31.69 Archive.seek :empty @12:00:46.35 15.7psi after compress @12:00:56.95 16.8psi after compress (stable) @12:00:56.98 Archive.in :gulp @12:00:57.09 Archive.seek :topVent @12:01:03.37 17.1psi after gulp @12:01:08.98 17.1psi after gulp (stable) @12:01:09.00 Archive stroke #2 of 8 @12:01:09.13 Archive.in :pressurize @12:01:09.22 Archive.seek :pressurized @12:01:21.76 16.9psi after pressurize @12:01:27.36 16.9psi after pressurize (stable) @12:01:27.39 Archive.in :compress @12:01:27.50 Archive.seek :empty @12:01:42.17 28.9psi after compress @12:01:53.27 30.6psi after compress (stable) @12:01:53.30 Archive.in :gulp @12:01:53.44 Archive.seek :topVent @12:01:59.74 30.9psi after gulp @12:02:05.34 30.9psi after gulp (stable) @12:02:05.36 Archive stroke #3 of 8 @12:02:05.49 Archive.in :pressurize @12:02:05.58 Archive.seek :pressurized @12:02:18.18 30.7psi after pressurize @12:02:24.17 30.5psi after pressurize (stable) @12:02:24.29 Archive.in :compress @12:02:24.39 Archive.seek :empty @12:02:39.06 42.4psi after compress @12:02:50.18 44.0psi after compress (stable) @12:02:50.21 Archive.in :gulp @12:02:50.31 Archive.seek :topVent @12:02:56.60 44.2psi after gulp @12:03:02.20 44.3psi after gulp (stable) @12:03:02.22 Archive stroke #4 of 8 @12:03:02.36 Archive.in :pressurize @12:03:02.49 Archive.seek :pressurized @12:03:15.03 44.1psi after pressurize @12:03:20.64 44.0psi after pressurize (stable) @12:03:20.67 Archive.in :compress @12:03:20.78 Archive.seek :empty @12:03:31.55 Reached 50.2 of 50.0psi after pumping 6835uL (3.95 strokes) @12:03:31.57 Archive.in :default @12:03:31.72 Archive.hold @12:03:31.84 Handoff.select :waste @12:03:45.51 delay 1:00 @12:04:45.54 Handoff.select :blocked @12:05:08.31 Handoff.select :waste @12:05:08.47 0.4psi at evacEnd @12:05:08.48 Handoff.select :blocked @12:05:08.64 Archive.in :fast @12:05:08.73 Archive.seek :topVent @12:05:22.15 Stable @0.5psi after Handoff blocked @12:05:22.18 Archive syringe pumping up to 8 strokes to reach 30psi @12:05:22.22 Archive stroke #1 of 8 @12:05:22.38 Archive.in :pressurize @12:05:22.48 Archive.seek :pressurized @12:05:35.02 1.7psi after pressurize @12:05:35.05 Archive.in :compress @12:05:35.16 Archive.seek :empty @12:05:49.84 12.9psi after compress @12:06:00.45 13.5psi after compress (stable) @12:06:00.48 Archive.in :gulp @12:06:00.58 Archive.seek :topVent @12:06:06.92 13.7psi after gulp @12:06:12.55 13.6psi after gulp (stable) @12:06:12.58 Archive stroke #2 of 8 @12:06:12.73 Archive.in :pressurize @12:06:12.83 Archive.seek :pressurized @12:06:25.35 13.5psi after pressurize @12:06:25.38 Archive.in :compress @12:06:25.48 Archive.seek :empty @12:06:40.14 25.5psi after compress @12:06:50.75 26.1psi after compress (stable) @12:06:50.78 Archive.in :gulp @12:06:50.88 Archive.seek :topVent @12:06:57.17 26.3psi after gulp @12:07:03.17 26.3psi after gulp (stable) @12:07:03.28 Archive stroke #3 of 8 @12:07:03.43 Archive.in :pressurize @12:07:03.51 Archive.seek :pressurized @12:07:16.07 26.1psi after pressurize @12:07:16.10 Archive.in :compress @12:07:16.20 Archive.seek :empty @12:07:22.94 Reached 30.6 of 30.5psi after pumping 5019uL (2.90 strokes) @12:07:22.96 Archive.in :default @12:07:23.07 Archive.hold @12:07:23.18 Handoff.select :waste @12:07:37.91 delay 1:00 @12:08:37.94 Handoff.select :blocked @12:09:00.67 Handoff.select :waste @12:09:00.82 0.5psi at evacEnd @12:09:00.87 Air.seek :fullVent @12:09:02.34 Archive.in :fast @12:09:02.43 Archive.seek :topVent @12:09:08.97 Seawater evacuated, Pressure above/below filter released. @12:09:09.09 Lysis1.in :slow @12:09:09.19 Lysis1.seek 1050uL @12:09:39.23 delay 5 seconds @12:09:44.25 Handoff.select :blocked @12:09:44.49 Lysis1.seek 450uL @12:10:44.46 delay 3 seconds @12:10:47.56 Air.in mix with 1.40048 parts Lysis1 @12:10:48.27 Air.seek :evac @12:10:48.31 Lysis1.seek 300uL @12:11:03.49 Lysis1.seek :empty @12:11:33.66 Lysis buffer delivered @11.0psi @12:11:33.68 Polling Can every 2:00 @12:11:34.25 PuckHeater.configure :default #ambient 23.5C @12:11:34.33 PuckHeater.seek 79.7C @12:12:26.11 PuckHeater.hold 2:00,79.7C @12:14:26.14 Polling Can every 2:00 @12:14:26.69 PuckHeater.configure :default #ambient 23.5C @12:14:26.78 PuckHeater.hold 30 seconds,78.6C @12:14:56.97 Polling Can every 2:00 @12:14:57.53 PuckHeater.configure :default #ambient 23.5C @12:14:57.64 PuckHeater.hold 30 seconds,78.1C @12:15:27.84 Polling Can every 2:00 @12:15:28.40 PuckHeater.configure :default #ambient 23.5C @12:15:28.48 PuckHeater.hold 10:00,77.6C @12:25:28.63 Lysis complete. Syringe pressure @15.3psi @12:25:28.66 Archive syringe pumping up to 5 strokes to reach 40psi @12:25:28.68 Archive stroke #1 of 5 @12:25:28.82 Archive.in :pressurize @12:25:28.90 Archive.seek :pressurized @12:25:41.42 15.0psi after pressurize @12:25:47.02 14.9psi after pressurize (stable) @12:25:47.05 Archive.in :compress @12:25:47.15 Archive.seek :empty @12:25:57.79 Reached 40.6 of 40.0psi after pumping 1641uL (0.95 strokes) @12:25:57.82 Archive.in :default @12:25:57.93 Archive.hold @12:25:58.03 delay 2 seconds @12:26:00.06 Archive.in :fast @12:26:00.15 Archive.seek :full @12:26:07.04 Lysis1.seek 138uL @12:26:21.48 delay 1 second @12:26:22.52 Archive.seek :full @12:26:22.56 Lysis1.seek :empty @12:26:36.52 Handoff.select :blocked @12:26:36.66 Initial air pressure @42.6psi @12:26:36.74 Lysis1.in :extract @12:26:36.90 Lysis1.seek 600uL,nil @12:26:41.59 Archive syringe pumping up to 6 strokes to reach 25psi @12:26:41.62 Archive stroke #1 of 6 @12:26:41.78 Archive.in :pressurize @12:26:41.90 Archive.seek :pressurized @12:26:42.50 Archive.in :default @12:26:42.58 Archive.hold @12:26:42.95 Initial 600uL lysate @13.3psi @12:26:42.97 Handoff.select :blocked @12:26:43.14 Initial air pressure @13.3psi @12:26:43.25 Lysis1.seek 825uL,nil @12:26:43.46 Archive syringe pumping up to 6 strokes to reach 17psi @12:26:43.47 Archive stroke #1 of 6 @12:26:43.72 Archive.in :pressurize @12:26:43.84 Archive.seek :pressurized @12:26:55.76 13.3psi after pressurize @12:27:01.40 13.2psi after pressurize (stable) @12:27:01.43 Archive.in :compress @12:27:01.57 Archive.seek :empty @12:27:04.70 Reached 17.1 of 17.0psi after pumping 1466uL (0.85 strokes) @12:27:04.72 Archive.in :default @12:27:04.88 Archive.hold @12:27:06.42 Archive syringe pumping up to 6 strokes to reach 17psi @12:27:06.44 Archive stroke #1 of 6 @12:27:06.58 Archive.in :compress @12:27:06.69 Archive.seek :empty @12:27:07.15 Archive.in :default @12:27:07.23 Archive.hold @12:27:07.54 Recovered 827uL lysate @13.9psi @12:27:07.57 Air.in :halfSpeed @12:27:07.74 Air.seek :fullVent @12:27:09.99 Puck Top Vented, bottom @17.0psi @12:27:10.01 Handoff.select :waste @12:27:10.18 delay 5 seconds @12:27:15.27 Handoff open to waste, bottom @0.4psi @12:27:15.30 Air.in :default @12:27:15.40 Air.seek :evac @12:27:16.85 Lysis2.in :DAmix @12:27:17.09 Lysis1.in :DAmix @12:27:17.87 Lysis2.seek 700uL @12:27:17.93 Lysis1.seek 576uL @12:27:53.07 delay 5 seconds @12:27:58.09 Handoff.select SPR @12:27:58.25 SPR.command "start 2 14" @12:27:59.14 SPR stabilized @12:27:59.33 delay 10 seconds @12:28:09.36 SPR.command "start 2 0" @12:28:12.51 Waiting up to 11:00 for SPR to enter state 1 @12:30:10.19 SPR.command "hold_on" @12:30:12.09 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:30:12.73 Lysis2.seek 400uL @12:30:12.82 Lysis1.seek 277uL @12:30:54.81 delay 15 seconds @12:31:09.84 SPR.command "hold_off" @12:31:13.17 Waiting up to 38:00 for SPR to enter state 7 @12:45:32.41 SPRsummary:-9.46RIU,2.48RIU,78.4RIU,75.1RIU,high,160ng/L,388ng/L after 3871s @12:57:02.83 SPR analysis completed @12:57:10.51 Ended log spr23aug0611h#2.spr.bz2 @12:57:10.53 SPR.command "stats_off" @12:57:11.02 Handoff.select :close @12:57:11.20 Gate.power :heat,:OFF @12:57:11.22 Gate.power :cartridge,:OFF @12:57:11.35 PinPort.in :unlocking @12:57:11.44 PinPort.seek :clear @12:57:13.39 Carousel.in :default @12:57:13.55 Carousel.home.seek 60 @12:57:13.95 Plunge.seek :locked @12:57:14.87 Archive.in :unlocking @12:57:15.08 Archive.seek :clear @12:57:15.15 Air.in :unlocking @12:57:15.38 Air.seek :clear @12:57:15.53 Lysis1.in :unlocking @12:57:15.70 Lysis1.seek :clear @12:57:15.85 Lysis2.in :unlocking @12:57:15.95 Lysis2.seek :clear @12:57:19.82 Gate.power :motor,:OFF @12:57:19.84 Gate.power :core,:OFF @12:57:19.89 Gate.power :analytic1,:OFF @12:57:19.91 Gate.power :analytic2,:OFF @12:57:19.95 Gate.power :main,:OFF @12:57:19.98 Safely stopped and ready to power off :STOPPED @12:58:25.12 -> exit @12:58:25.15 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @12:58:44.59 Session Begun @12:58:47.92 -> exit @12:58:47.94 Session Ended @12:58:53.42 Session Begun @12:58:57.07 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 827, spr: spr23aug0611h#2.spr.bz2}} @12:59:05.78 -> Cartridge.unlock @12:59:05.87 BusOff in bill -- core bus was not powered while reading Handoff Pin Port (PP)'s status @12:59:18.19 -> ESP.ready! @12:59:18.25 Gate.power :sampler,:OFF,limit to 3500mA @12:59:18.28 Gate.power :main,:ON,limit to 5000mA @12:59:18.31 Gate.power :analytic1,:ON,limit to 5000mA @12:59:18.35 Gate.power :analytic2,:ON,limit to 5000mA @12:59:18.44 Powering core bus @12:59:20.81 SPR Module 1, FW Rev. B, FPGA Rev. C @12:59:21.47 Archive.configure :default @12:59:21.59 Air.configure :default @12:59:21.72 Lysis1.configure :default @12:59:21.81 Lysis2.configure :default @12:59:21.94 Plunge.configure :default @12:59:22.06 SamplePump.configure :default @12:59:22.17 Toroid.configure :default @12:59:22.29 Carousel.configure :default @12:59:22.40 Pressure.configure :default @12:59:22.53 ExtraPump.configure :default @12:59:22.62 PinPort.configure :default @12:59:22.71 All elf controllers running firmware version 4.84 @12:59:22.74 Gate.power :motor,:ON,limit to 5000mA @12:59:23.00 Archive.home.jog 0 @12:59:23.89 PinPort.home.jog 0 @12:59:23.93 Toroid.home.jog 0 @12:59:23.99 Plunge.home.jog 0 @12:59:24.03 Lysis1.home.jog 0 @12:59:24.07 Air.home.jog 0 @12:59:24.12 Carousel.home.jog 0 @12:59:24.16 Lysis2.home.jog 0 @12:59:25.15 Archive.in :homing @12:59:25.47 Archive.jog 1385 @12:59:25.56 PinPort.in :homing @12:59:25.60 Plunge.in :homing @12:59:25.67 Lysis1.in :homing @12:59:25.77 Lysis2.in :homing @12:59:25.82 Toroid.in :homing @12:59:25.87 Air.in :homing @12:59:26.19 Plunge.jog 1562 @12:59:26.31 PinPort.jog -109 @12:59:26.38 Toroid.jog 611 @12:59:26.41 Lysis2.jog 1393 @12:59:26.47 Air.jog 1370 @12:59:26.57 Lysis1.jog 1388 @12:59:27.01 PinPort.setRawPosition! 0 @12:59:27.08 PinPort.seek :home @12:59:27.22 Archive.setRawPosition! 50 @12:59:27.25 PinPort.in :default @12:59:27.38 Archive.seek :home @12:59:27.72 Archive.in :default @12:59:27.85 Plunge.setRawPosition! 50 @12:59:27.94 Plunge.seek :home @12:59:28.07 Lysis2.setRawPosition! 50 @12:59:28.12 Lysis1.setRawPosition! 50 @12:59:28.15 Air.setRawPosition! 50 @12:59:28.35 Lysis2.seek :home @12:59:28.38 Air.seek :home @12:59:28.44 Lysis1.seek :home @12:59:28.70 Plunge.in :default @12:59:28.90 Lysis2.in :default @12:59:28.92 Lysis1.in :default @12:59:28.97 Toroid.setRawPosition! 0 @12:59:29.04 Air.in :default @12:59:29.29 Toroid.seek :home @12:59:29.42 Toroid.in :default @12:59:29.57 Toroid.seek :clear @12:59:31.24 Carousel.in :default @12:59:31.40 Carousel.home.seek 60 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Pressure = Absolute Pressure (Leak) Sensor @17psia PinPort = Handoff Pin Port (PP) clear @13:00:58.00 -> slot [60] =>[:dry, :microSPR_phyto] {60 => { Type: :microSPR_phyto, State: :dry}} @13:01:00.38 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry}} @13:03:26.40 -> fire :microSPR_phyto @13:03:27.26 Toroid.seek :clear @13:03:27.38 Carousel.in :default @13:03:27.54 Carousel.home.seek 60 @13:03:27.88 Selecting Cartridge 60 @13:03:28.22 Carousel.seek 60 @13:03:28.51 Lysis1.in :fast @13:03:28.61 Lysis1.seek :locked @13:03:31.27 Plunge.seek :locked @13:03:31.49 Air.in :fast @13:03:31.58 Air.seek :locked @13:03:33.21 Carousel.reconfigure :locked @13:03:33.40 Powering cartridge bus @13:03:36.07 Gate.setIR 1750,5000,0,6 @13:03:36.20 Gate.setIR 0,5000,0,6 @13:03:36.29 PuckHeater.configure :default #ambient 23.5C @13:03:36.38 Gate.power :heat,:ON,limit to 5000mA @13:03:37.09 Mounted lysis Cartridge board#Q05053 @13:03:37.15 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:03:37.52 Preparing to filter cartridge 60 @13:03:37.83 Gate.power :sampler,:ON @13:03:37.96 Priming sample loop w/150ml, bypass w/50ml @13:03:37.98 SamplePump.setPosition! 0ml @13:03:38.06 Bypass.open @13:03:39.58 Intake.open @13:03:40.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:03:43.09 Vacuum tare is 56% of full scale @13:03:43.28 Pressure.in :default @13:03:43.37 Pressure.hold @13:03:43.67 Waiting for external sampler to finish priming... @13:03:43.69 delayUntil "13:07:07.85" @13:07:07.90 SamplePump.in :prime @13:07:08.05 SamplePump.seek 50ml,2:53 @13:08:09.05 Pressure.stop @13:08:09.13 SamplePump.setPosition! 0ml @13:08:09.21 Exhaust.open @13:08:10.68 Bypass.close @13:08:12.26 Pressure.in :default @13:08:12.35 Pressure.hold @13:08:12.69 SamplePump.in :prime @13:08:12.78 SamplePump.seek 150ml,7:39 @13:11:15.09 Pressure.stop @13:11:15.24 Bypass.open @13:11:16.00 Intake.close @13:11:16.81 Toroid.in :hold @13:11:16.90 Toroid.seek :crack @13:11:18.47 Toroid.seek :cartridge @13:11:19.63 Toroid.in :default @13:11:19.74 Toroid.seek :clear @13:11:21.82 Pressurizing cartridge to 26psi above 15.6psia @13:11:21.84 SamplePump.setPosition! 0ml @13:11:21.93 Intake.open @13:11:22.62 Bypass.close @13:11:23.31 Exhaust.close @13:11:24.19 Pressure.in :default @13:11:24.28 Pressure.hold @13:11:24.55 SamplePump.in :charge @13:11:24.65 SamplePump.seek 2.3ml,42 seconds @13:11:39.00 Pressure.stop @13:11:39.09 Intake.close @13:11:39.89 Pressurized cartridge with 0.929ml to 26.1psi @13:11:39.91 Isolated @32.5psia (16.9psi) @13:11:39.94 Verifying cartridge holds at least 0.65..0.85 of 32.5psia @13:11:40.01 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:11:44.69 Sampling 50.0ml @13:11:44.71 SamplePump.setPosition! 0ml @13:11:44.81 Exhaust.open @13:11:45.55 Intake.open @13:11:46.43 Pressure.in :default @13:11:46.53 Pressure.hold @13:11:46.70 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @13:11:46.93 SamplePump.in :sample @13:11:47.03 SamplePump.seek 50ml,4:41 @13:11:50.06 Cartridge 60 filtering 1/50ml at 0.59ml/s with 25.5psi vacuum @13:12:50.06 Cartridge 60 filtering 33/50ml at 0.54ml/s with 28.2psi vacuum @13:13:22.69 Pressure.stop @13:13:22.92 Intake.close @13:13:22.99 Exhaust.close @13:13:23.32 Toroid.in :hold @13:13:23.41 Toroid.seek :bypass @13:13:25.08 Toroid.in :default @13:13:25.19 Toroid.seek :clear @13:13:26.83 Sampled 50.0ml @13:13:28.56 SPR.command "read_sequences" @13:13:29.07 SPR.command "stats_on" @13:13:29.25 SPR.command "start 0 1" @13:13:29.95 SPR.start /var/log/esp/spr23aug0613h.spr.bz2 @13:13:30.01 Gate.power :sampler,:OFF @13:13:31.92 Handoff.select :waste @13:13:38.66 Syringe tare is 18% of full scale @13:13:38.69 PinPort.in :locking @13:13:38.81 PinPort.seek :locked @13:13:41.38 Archive.in :fast @13:13:41.51 Lysis2.in :fast @13:13:41.59 Lysis1.seek 1350uL @13:13:41.64 Archive.seek :full @13:13:41.82 Lysis2.seek 950uL @13:13:46.66 Plunge.seek :reagent @13:13:47.65 Evacuating puck with filter bubble point of 50psi @13:13:47.69 PinPort.seek :locked @13:13:47.83 Handoff.select :waste @13:13:54.45 Air syringe pumping up to 1 strokes to reach 8psi @13:13:54.46 Air stroke #1 of 1 @13:13:54.61 Air.in :pressurize @13:13:54.70 Air.seek :pressurized @13:14:00.48 3.9psi after pressurize @13:14:00.51 Air.in :compress @13:14:00.62 Air.seek :evac @13:14:03.75 2.5psi after compress @13:14:15.35 0.4psi after compress (stable) @13:14:15.37 Air.in :default @13:14:15.48 Air.hold @13:14:15.77 at 0.3psi Opened to waste @13:14:15.80 Handoff.select :blocked @13:14:15.99 Archive syringe pumping up to 8 strokes to reach 50psi @13:14:16.01 Archive stroke #1 of 8 @13:14:16.14 Archive.in :pressurize @13:14:16.23 Archive.seek :pressurized @13:14:28.77 0.8psi after pressurize @13:14:34.37 0.7psi after pressurize (stable) @13:14:34.40 Archive.in :compress @13:14:34.51 Archive.seek :empty @13:14:49.16 15.0psi after compress @13:14:59.76 16.3psi after compress (stable) @13:14:59.79 Archive.in :gulp @13:14:59.90 Archive.seek :topVent @13:15:06.19 16.7psi after gulp @13:15:12.79 16.1psi after gulp (stable) @13:15:12.80 Archive stroke #2 of 8 @13:15:12.94 Archive.in :pressurize @13:15:13.03 Archive.seek :pressurized @13:15:25.55 15.7psi after pressurize @13:15:31.15 15.6psi after pressurize (stable) @13:15:31.18 Archive.in :compress @13:15:31.86 Archive.seek :empty @13:15:46.52 26.3psi after compress @13:15:57.15 27.5psi after compress (stable) @13:15:57.20 Archive.in :gulp @13:15:57.30 Archive.seek :topVent @13:16:03.61 27.8psi after gulp @13:16:09.24 27.8psi after gulp (stable) @13:16:09.26 Archive stroke #3 of 8 @13:16:09.41 Archive.in :pressurize @13:16:09.53 Archive.seek :pressurized @13:16:22.10 27.5psi after pressurize @13:16:27.71 27.5psi after pressurize (stable) @13:16:27.73 Archive.in :compress @13:16:27.85 Archive.seek :empty @13:16:42.50 38.6psi after compress @13:16:53.12 39.8psi after compress (stable) @13:16:53.15 Archive.in :gulp @13:16:53.26 Archive.seek :topVent @13:17:00.08 40.0psi after gulp @13:17:05.71 40.0psi after gulp (stable) @13:17:05.73 Archive stroke #4 of 8 @13:17:05.86 Archive.in :pressurize @13:17:05.96 Archive.seek :pressurized @13:17:18.52 39.9psi after pressurize @13:17:24.15 39.8psi after pressurize (stable) @13:17:24.20 Archive.in :compress @13:17:24.36 Archive.seek :empty @13:17:38.78 Reached 50.1 of 50.0psi after pumping 6914uL (4.00 strokes) @13:17:38.80 Archive.in :default @13:17:38.90 Archive.hold @13:17:39.01 Handoff.select :waste @13:17:49.24 delay 1:00 @13:18:49.27 Handoff.select :blocked @13:19:12.06 Handoff.select :waste @13:19:12.21 0.4psi at evacEnd @13:19:12.23 Handoff.select :blocked @13:19:12.40 Archive.in :fast @13:19:12.49 Archive.seek :topVent @13:19:26.22 Stable @0.6psi after Handoff blocked @13:19:26.26 Archive syringe pumping up to 8 strokes to reach 31psi @13:19:26.33 Archive stroke #1 of 8 @13:19:27.03 Archive.in :pressurize @13:19:27.15 Archive.seek :pressurized @13:19:39.74 0.9psi after pressurize @13:19:39.77 Archive.in :compress @13:19:39.89 Archive.seek :empty @13:19:54.57 12.4psi after compress @13:20:05.18 13.3psi after compress (stable) @13:20:05.21 Archive.in :gulp @13:20:05.32 Archive.seek :topVent @13:20:11.69 13.5psi after gulp @13:20:17.32 13.5psi after gulp (stable) @13:20:17.33 Archive stroke #2 of 8 @13:20:17.47 Archive.in :pressurize @13:20:17.56 Archive.seek :pressurized @13:20:30.13 13.3psi after pressurize @13:20:30.16 Archive.in :compress @13:20:30.26 Archive.seek :empty @13:20:44.92 24.8psi after compress @13:20:55.52 25.5psi after compress (stable) @13:20:55.55 Archive.in :gulp @13:20:55.69 Archive.seek :topVent @13:21:01.98 25.8psi after gulp @13:21:07.60 25.8psi after gulp (stable) @13:21:07.64 Archive stroke #3 of 8 @13:21:07.78 Archive.in :pressurize @13:21:07.87 Archive.seek :pressurized @13:21:20.39 25.5psi after pressurize @13:21:20.42 Archive.in :compress @13:21:20.52 Archive.seek :empty @13:21:29.07 Reached 30.7 of 30.6psi after pumping 5057uL (2.92 strokes) @13:21:29.12 Archive.in :default @13:21:29.30 Archive.hold @13:21:29.48 Handoff.select :waste @13:21:42.23 delay 1:00 @13:22:42.25 Handoff.select :blocked @13:23:04.98 Handoff.select :waste @13:23:05.13 0.3psi at evacEnd @13:23:05.15 Air.seek :fullVent @13:23:06.61 Archive.in :fast @13:23:06.70 Archive.seek :topVent @13:23:13.37 Seawater evacuated, Pressure above/below filter released. @13:23:13.49 Lysis1.in :slow @13:23:13.58 Lysis1.seek 1050uL @13:23:43.66 delay 5 seconds @13:23:48.68 Handoff.select :blocked @13:23:48.92 Lysis1.seek 450uL @13:24:49.39 delay 3 seconds @13:24:52.53 Air.in mix with 1.40048 parts Lysis1 @13:24:53.21 Air.seek :evac @13:24:53.30 Lysis1.seek 300uL @13:25:08.45 Lysis1.seek :empty @13:25:38.60 Lysis buffer delivered @9.1psi @13:25:38.62 Polling Can every 2:00 @13:25:39.19 PuckHeater.configure :default #ambient 23.8C @13:25:39.27 PuckHeater.seek 79.7C @13:26:27.86 PuckHeater.hold 2:00,79.7C @13:28:27.99 Polling Can every 2:00 @13:28:28.54 PuckHeater.configure :default #ambient 23.8C @13:28:28.63 PuckHeater.hold 30 seconds,78.6C @13:28:58.83 Polling Can every 2:00 @13:28:59.38 PuckHeater.configure :default #ambient 23.9C @13:28:59.47 PuckHeater.hold 30 seconds,78.1C @13:29:29.68 Polling Can every 2:00 @13:29:30.64 PuckHeater.configure :default #ambient 23.8C @13:29:30.85 PuckHeater.hold 10:00,77.6C @13:39:30.44 Lysis complete. Syringe pressure @12.9psi @13:39:30.47 Archive syringe pumping up to 5 strokes to reach 40psi @13:39:30.55 Archive stroke #1 of 5 @13:39:30.69 Archive.in :pressurize @13:39:30.77 Archive.seek :pressurized @13:39:43.36 12.5psi after pressurize @13:39:48.96 12.5psi after pressurize (stable) @13:39:48.99 Archive.in :compress @13:39:49.12 Archive.seek :empty @13:40:01.50 Reached 40.5 of 40.0psi after pumping 1679uL (0.97 strokes) @13:40:01.52 Archive.in :default @13:40:01.64 Archive.hold @13:40:02.26 delay 2 seconds @13:40:04.28 Archive.in :fast @13:40:04.38 Archive.seek :full @13:40:11.41 Lysis1.seek 138uL @13:40:25.32 delay 1 second @13:40:26.36 Archive.seek :full @13:40:26.45 Lysis1.seek :empty @13:40:40.43 Handoff.select :blocked @13:40:40.56 Initial air pressure @41.8psi @13:40:40.65 Lysis1.in :extract @13:40:40.81 Lysis1.seek 600uL,nil @13:40:46.38 Archive syringe pumping up to 6 strokes to reach 25psi @13:40:46.39 Archive stroke #1 of 6 @13:40:46.51 Archive.in :default @13:40:46.59 Archive.hold @13:40:46.82 Initial 600uL lysate @15.2psi @13:40:46.85 Handoff.select :blocked @13:40:47.00 Initial air pressure @15.4psi @13:40:47.10 Lysis1.seek 825uL,nil @13:40:47.89 Archive syringe pumping up to 6 strokes to reach 17psi @13:40:47.91 Archive stroke #1 of 6 @13:40:48.16 Archive.in :pressurize @13:40:48.34 Archive.seek :pressurized @13:41:00.87 15.1psi after pressurize @13:41:24.03 16.0psi after pressurize (stable) @13:41:24.09 Archive.in :compress @13:41:24.23 Archive.seek :empty @13:41:26.94 Archive.in :default @13:41:27.02 Archive.hold @13:41:27.32 Recovered 825uL lysate @16.1psi @13:41:27.36 Air.in :halfSpeed @13:41:27.49 Air.seek :fullVent @13:41:29.75 Puck Top Vented, bottom @10.6psi @13:41:29.76 Handoff.select :waste @13:41:29.93 delay 5 seconds @13:41:35.03 Handoff open to waste, bottom @0.5psi @13:41:35.09 Air.in :default @13:41:35.25 Air.seek :evac @13:41:36.70 Lysis2.in :DAmix @13:41:37.30 Lysis1.in :DAmix @13:41:38.02 Lysis2.seek 700uL @13:41:38.09 Lysis1.seek 575uL @13:42:13.22 delay 5 seconds @13:42:18.24 Handoff.select SPR @13:42:18.41 SPR.command "start 2 14" @13:42:19.01 SPR stabilized @13:42:19.20 delay 10 seconds @13:42:29.23 SPR.command "start 2 0" @13:42:32.38 Waiting up to 11:00 for SPR to enter state 1 @13:44:30.05 SPR.command "hold_on" @13:44:31.43 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @13:44:32.12 Lysis2.seek 400uL @13:44:32.18 Lysis1.seek 275uL @13:45:14.28 delay 15 seconds @13:45:29.31 SPR.command "hold_off" @13:45:32.08 Waiting up to 38:00 for SPR to enter state 7 @13:59:52.26 SPRsummary:-483RIU,4e+09RIU,107RIU,830RIU,none,none,none after 2939s @14:11:22.69 SPR analysis completed @14:11:30.84 Ended log spr23aug0613h.spr.bz2 @14:11:30.87 SPR.command "stats_off" @14:11:31.85 Handoff.select :close @14:11:32.02 Gate.power :heat,:OFF @14:11:32.05 Gate.power :cartridge,:OFF @14:11:32.18 PinPort.in :unlocking @14:11:32.27 PinPort.seek :clear @14:11:34.22 Carousel.in :default @14:11:34.38 Carousel.home.seek 60 @14:11:34.75 Plunge.seek :locked @14:11:35.72 Archive.in :unlocking @14:11:35.93 Archive.seek :clear @14:11:36.01 Air.in :unlocking @14:11:36.20 Air.seek :clear @14:11:36.36 Lysis1.in :unlocking @14:11:36.52 Lysis1.seek :clear @14:11:37.13 Lysis2.in :unlocking @14:11:37.23 Lysis2.seek :clear @14:11:41.06 Gate.power :motor,:OFF @14:11:41.09 Gate.power :core,:OFF @14:11:41.13 Gate.power :analytic1,:OFF @14:11:41.16 Gate.power :analytic2,:OFF @14:11:41.19 Gate.power :main,:OFF @14:11:41.23 Safely stopped and ready to power off :STOPPED @14:11:56.96 -> PV.goHome! @14:11:57.04 BusOff in bill -- Plunge Valve (PV) is not powered! @14:12:12.52 -> ESP.ready! @14:12:12.58 Gate.power :sampler,:OFF,limit to 3500mA @14:12:12.61 Gate.power :main,:ON,limit to 5000mA @14:12:12.65 Gate.power :analytic1,:ON,limit to 5000mA @14:12:12.69 Gate.power :analytic2,:ON,limit to 5000mA @14:12:12.79 Powering core bus @14:12:15.15 SPR Module 1, FW Rev. B, FPGA Rev. C @14:12:15.60 Archive.configure :default @14:12:15.76 Air.configure :default @14:12:15.85 Lysis1.configure :default @14:12:15.94 Lysis2.configure :default @14:12:16.07 Plunge.configure :default @14:12:16.19 SamplePump.configure :default @14:12:16.30 Toroid.configure :default @14:12:16.42 Carousel.configure :default @14:12:16.55 Pressure.configure :default @14:12:16.67 ExtraPump.configure :default @14:12:16.76 PinPort.configure :default @14:12:16.84 All elf controllers running firmware version 4.84 @14:12:16.87 Gate.power :motor,:ON,limit to 5000mA @14:12:17.19 Archive.home.jog 0 @14:12:18.03 PinPort.home.jog 0 @14:12:18.09 Toroid.home.jog 0 @14:12:18.14 Plunge.home.jog 0 @14:12:18.18 Lysis1.home.jog 0 @14:12:18.25 Lysis2.home.jog 0 @14:12:18.27 Air.home.jog 0 @14:12:18.32 Carousel.home.jog 0 @14:12:19.16 Archive.in :homing @14:12:19.48 Archive.jog 1386 @14:12:19.75 Toroid.in :homing @14:12:19.88 Lysis2.in :homing @14:12:19.92 Air.in :homing @14:12:19.99 PinPort.in :homing @14:12:20.05 Lysis1.in :homing @14:12:20.11 Plunge.in :homing @14:12:20.41 Toroid.jog 611 @14:12:20.47 Lysis2.jog 1388 @14:12:20.52 PinPort.jog -109 @14:12:20.56 Air.jog 1369 @14:12:20.62 Plunge.jog 1568 @14:12:20.67 Lysis1.jog 1388 @14:12:21.15 Archive.setRawPosition! 50 @14:12:21.18 PinPort.setRawPosition! 0 @14:12:21.29 Archive.seek :home @14:12:21.35 PinPort.seek :home @14:12:21.57 PinPort.in :default @14:12:21.74 Archive.in :default @14:12:21.96 Lysis2.setRawPosition! 50 @14:12:22.49 Lysis2.seek :home @14:12:22.73 Lysis1.setRawPosition! 50 @14:12:22.76 Air.setRawPosition! 50 @14:12:22.80 Plunge.setRawPosition! 50 @14:12:23.00 Lysis1.seek :home @14:12:23.03 Air.seek :home @14:12:23.09 Plunge.seek :home @14:12:23.40 Toroid.setRawPosition! 0 @14:12:23.43 Lysis2.in :default @14:12:23.61 Toroid.seek :home @14:12:23.80 Plunge.in :default @14:12:23.84 Lysis1.in :default @14:12:23.91 Toroid.in :default @14:12:23.95 Air.in :default @14:12:24.47 Toroid.seek :clear @14:12:26.11 Carousel.in :default @14:12:26.26 Carousel.home.seek 60 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Pressure = Absolute Pressure (Leak) Sensor @17psia PinPort = Handoff Pin Port (PP) clear @14:12:30.74 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0613h.spr.bz2}} @14:12:50.08 -> slot [60] =>[:dry, :microSPR_phyto] {60 => { Type: :microSPR_phyto, State: :dry}} @14:12:52.42 -> slot {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry}} @14:13:11.62 -> Cartridge.reserve 60..1 1 @14:13:14.80 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}} @14:14:22.71 -> SC.to 25 @14:14:22.74 Carousel.seek 25 Storage Carousel (SC) at 25 @14:23:41.03 -> SC.to 1;delay 2;SC.to 60;delay 2;SC.to 1 @14:23:41.07 Carousel.seek 1 @14:23:46.16 delay 2 seconds @14:23:48.19 Carousel.seek 60 @14:24:00.40 delay 2 seconds @14:24:02.43 Carousel.seek 1 Storage Carousel (SC) at 1 @14:26:34.70 -> SC.to 1;delay 2;SC.to 60;delay 2;SC.to 1 @14:26:34.73 Carousel.seek 1 @14:26:34.83 delay 2 seconds @14:26:36.86 Carousel.seek 60 @14:26:49.07 delay 2 seconds @14:26:51.10 Carousel.seek 1 Storage Carousel (SC) at 1 @14:27:28.46 -> SC.to 1;delay 2;SC.to 60;delay 2;SC.to 1 @14:27:28.49 Carousel.seek 1 @14:27:28.61 delay 2 seconds @14:27:30.64 Carousel.seek 60 @14:27:42.85 delay 2 seconds @14:27:44.88 Carousel.seek 1 Storage Carousel (SC) at 1 @14:31:42.29 -> cleanToroid :cleanToroid @14:31:50.50 -> require 'vacuumCart' true @14:31:53.78 -> cleanToroid @14:31:53.79 Cleans Dry SC positions in slots, begin in 10% Bleach @14:31:55.16 Toroid.seek :clear @14:31:55.28 Carousel.in :default @14:31:55.44 Carousel.home.seek 1 @14:31:55.77 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:32:06.56 UserAbort in bill -- User Abort!! @14:32:09.77 -> slots {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}} @14:32:29.94 -> slots 1..60=>:dry {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, was: :standard}} @14:43:39.83 -> cleanToroid @14:43:39.86 Cleans Dry SC positions in slots, begin in 10% Bleach @14:43:40.73 Toroid.seek :clear @14:43:40.84 Carousel.in :default @14:43:40.99 Carousel.home.seek 1 @14:43:41.31 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:43:44.03 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @14:43:44.15 Priming sample loop w/100ml, bypass w/50ml @14:43:44.17 SamplePump.setPosition! 0ml @14:43:44.25 Bypass.open @14:43:45.77 Intake.open @14:43:46.57 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:43:49.16 Vacuum tare is 56% of full scale @14:43:49.35 Pressure.in :default @14:43:49.43 Pressure.hold @14:43:49.71 Gate.power :sampler,:ON @14:43:49.74 Waiting for external sampler to finish priming... @14:43:49.77 delayUntil "14:47:19.73" @14:44:06.84 SamplePump.stop @14:44:06.96 Intake.close @14:44:07.07 Bypass.close @14:44:07.93 Pressure.stop @14:44:08.03 UserAbort in bill -- User Abort!! @14:44:18.47 -> PumpPrimeDelay=0 0 @14:44:24.52 -> cleanToroid @14:44:24.54 Cleans Dry SC positions in slots, begin in 10% Bleach @14:44:25.38 Toroid.seek :clear @14:44:25.50 Carousel.in :default @14:44:25.69 Carousel.home.seek 1 @14:44:26.40 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:44:27.52 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @14:44:27.67 Priming sample loop w/100ml, bypass w/50ml @14:44:27.69 SamplePump.setPosition! 0ml @14:44:27.78 Bypass.open @14:44:29.31 Intake.open @14:44:30.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:44:32.70 Vacuum tare is 56% of full scale @14:44:32.89 Pressure.in :default @14:44:32.98 Pressure.hold @14:44:33.24 Waiting for external sampler to finish priming... @14:44:33.26 delayUntil "14:47:19.73" @14:45:00.14 SamplePump.stop @14:45:00.31 Intake.close @14:45:00.42 Bypass.close @14:45:01.19 Pressure.stop @14:45:01.29 UserAbort in bill -- User Abort!! @14:45:06.91 -> Sampler::PumpPrimeDelay -3:30 @14:45:11.07 -> Sampler::PumpPrimeDelay=0 @14:45:11.10 RuntimeError in bill -- cannot redefine constant PumpPrimeDelay @14:45:17.63 -> Sampler::PumpPrimeDelay=1 @14:45:17.69 RuntimeError in bill -- cannot redefine constant PumpPrimeDelay @14:45:35.31 -> cleanToroid @14:45:35.33 Cleans Dry SC positions in slots, begin in 10% Bleach @14:45:36.21 Toroid.seek :clear @14:45:36.32 Carousel.in :default @14:45:36.48 Carousel.home.seek 1 @14:45:36.78 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:45:37.92 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @14:45:38.04 Priming sample loop w/100ml, bypass w/50ml @14:45:38.06 SamplePump.setPosition! 0ml @14:45:38.14 Bypass.open @14:45:39.71 Intake.open @14:45:40.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:45:43.09 Vacuum tare is 56% of full scale @14:45:43.28 Pressure.in :default @14:45:43.37 Pressure.hold @14:45:43.67 Waiting for external sampler to finish priming... @14:45:43.69 delayUntil "14:47:19.73" @14:47:19.79 SamplePump.in :prime @14:47:19.94 SamplePump.seek 50ml,2:53 @14:48:20.95 Pressure.stop @14:48:21.57 SamplePump.setPosition! 0ml @14:48:21.68 Exhaust.open @14:48:23.14 Bypass.close @14:48:24.76 Pressure.in :default @14:48:24.85 Pressure.hold @14:48:25.20 SamplePump.in :prime @14:48:25.30 SamplePump.seek 100ml,5:16 @14:50:26.90 Pressure.stop @14:50:26.98 Opening all dry Cartridges @14:50:27.03 Ensuring (60) cartridge(s) open @14:50:27.37 Carousel.seek 1 @14:50:27.51 Toroid.in :hold @14:50:27.60 Toroid.seek :cartridge @14:50:29.50 Toroid.in :default @14:50:29.60 Toroid.seek :clear @14:50:31.78 Carousel.seek 2 @14:50:32.22 Toroid.in :hold @14:50:32.31 Toroid.seek :cartridge @14:50:34.17 Toroid.in :default @14:50:34.27 Toroid.seek :clear @14:50:36.39 Carousel.seek 3 @14:50:36.83 Toroid.in :hold @14:50:36.92 Toroid.seek :cartridge @14:50:38.78 Toroid.in :default @14:50:38.88 Toroid.seek :clear @14:50:41.01 Carousel.seek 4 @14:50:41.44 Toroid.in :hold @14:50:41.54 Toroid.seek :cartridge @14:50:43.42 Toroid.in :default @14:50:43.51 Toroid.seek :clear @14:50:45.67 Carousel.seek 5 @14:50:46.10 Toroid.in :hold @14:50:46.19 Toroid.seek :cartridge @14:50:48.06 Toroid.in :default @14:50:48.15 Toroid.seek :clear @14:50:50.81 Carousel.seek 6 @14:50:51.25 Toroid.in :hold @14:50:51.35 Toroid.seek :cartridge @14:50:53.21 Toroid.in :default @14:50:53.31 Toroid.seek :clear @14:50:55.43 Carousel.seek 7 @14:50:55.85 Toroid.in :hold @14:50:55.94 Toroid.seek :cartridge @14:50:57.81 Toroid.in :default @14:50:57.91 Toroid.seek :clear @14:51:00.06 Carousel.seek 8 @14:51:00.49 Toroid.in :hold @14:51:00.59 Toroid.seek :cartridge @14:51:02.45 Toroid.in :default @14:51:02.55 Toroid.seek :clear @14:51:04.67 Carousel.seek 9 @14:51:05.11 Toroid.in :hold @14:51:05.20 Toroid.seek :cartridge @14:51:07.07 Toroid.in :default @14:51:07.16 Toroid.seek :clear @14:51:09.27 Carousel.seek 10 @14:51:09.70 Toroid.in :hold @14:51:09.80 Toroid.seek :cartridge @14:51:11.69 Toroid.in :default @14:51:11.78 Toroid.seek :clear @14:51:13.94 Carousel.seek 11 @14:51:14.38 Toroid.in :hold @14:51:14.47 Toroid.seek :cartridge @14:51:16.33 Toroid.in :default @14:51:16.43 Toroid.seek :clear @14:51:18.56 Carousel.seek 12 @14:51:18.98 Toroid.in :hold @14:51:19.07 Toroid.seek :cartridge @14:51:21.52 Toroid.in :default @14:51:21.63 Toroid.seek :clear @14:51:23.81 Carousel.seek 13 @14:51:24.22 Toroid.in :hold @14:51:24.32 Toroid.seek :cartridge @14:51:26.18 Toroid.in :default @14:51:26.28 Toroid.seek :clear @14:51:28.40 Carousel.seek 14 @14:51:28.83 Toroid.in :hold @14:51:28.92 Toroid.seek :cartridge @14:51:30.78 Toroid.in :default @14:51:30.88 Toroid.seek :clear @14:51:33.01 Carousel.seek 15 @14:51:33.44 Toroid.in :hold @14:51:33.54 Toroid.seek :cartridge @14:51:35.39 Toroid.in :default @14:51:35.55 Toroid.seek :clear @14:51:37.73 Carousel.seek 16 @14:51:38.16 Toroid.in :hold @14:51:38.25 Toroid.seek :cartridge @14:51:40.11 Toroid.in :default @14:51:40.21 Toroid.seek :clear @14:51:42.32 Carousel.seek 17 @14:51:42.75 Toroid.in :hold @14:51:42.86 Toroid.seek :cartridge @14:51:44.72 Toroid.in :default @14:51:44.82 Toroid.seek :clear @14:51:46.94 Carousel.seek 18 @14:51:47.37 Toroid.in :hold @14:51:47.46 Toroid.seek :cartridge @14:51:49.31 Toroid.in :default @14:51:49.40 Toroid.seek :clear @14:51:52.10 Carousel.seek 19 @14:51:52.53 Toroid.in :hold @14:51:52.63 Toroid.seek :cartridge @14:51:54.49 Toroid.in :default @14:51:54.59 Toroid.seek :clear @14:51:56.72 Carousel.seek 20 @14:51:57.15 Toroid.in :hold @14:51:57.24 Toroid.seek :cartridge @14:51:59.11 Toroid.in :default @14:51:59.20 Toroid.seek :clear @14:52:01.33 Carousel.seek 21 @14:52:01.76 Toroid.in :hold @14:52:01.86 Toroid.seek :cartridge @14:52:03.72 Toroid.in :default @14:52:03.82 Toroid.seek :clear @14:52:05.97 Carousel.seek 22 @14:52:06.39 Toroid.in :hold @14:52:06.49 Toroid.seek :cartridge @14:52:08.35 Toroid.in :default @14:52:08.45 Toroid.seek :clear @14:52:10.58 Carousel.seek 23 @14:52:11.02 Toroid.in :hold @14:52:11.12 Toroid.seek :cartridge @14:52:12.97 Toroid.in :default @14:52:13.07 Toroid.seek :clear @14:52:15.19 Carousel.seek 24 @14:52:15.64 Toroid.in :hold @14:52:15.75 Toroid.seek :cartridge @14:52:17.63 Toroid.in :default @14:52:17.74 Toroid.seek :clear @14:52:19.91 Carousel.seek 25 @14:52:20.34 Toroid.in :hold @14:52:20.43 Toroid.seek :cartridge @14:52:22.73 Toroid.in :default @14:52:22.91 Toroid.seek :clear @14:52:25.04 Carousel.seek 26 @14:52:25.45 Toroid.in :hold @14:52:25.54 Toroid.seek :cartridge @14:52:27.44 Toroid.in :default @14:52:27.53 Toroid.seek :clear @14:52:29.69 Carousel.seek 27 @14:52:30.12 Toroid.in :hold @14:52:30.21 Toroid.seek :cartridge @14:52:32.07 Toroid.in :default @14:52:32.17 Toroid.seek :clear @14:52:34.29 Carousel.seek 28 @14:52:34.72 Toroid.in :hold @14:52:34.82 Toroid.seek :cartridge @14:52:36.68 Toroid.in :default @14:52:36.78 Toroid.seek :clear @14:52:38.90 Carousel.seek 29 @14:52:39.33 Toroid.in :hold @14:52:39.43 Toroid.seek :cartridge @14:52:41.29 Toroid.in :default @14:52:41.38 Toroid.seek :clear @14:52:43.51 Carousel.seek 30 @14:52:43.95 Toroid.in :hold @14:52:44.05 Toroid.seek :cartridge @14:52:45.92 Toroid.in :default @14:52:46.01 Toroid.seek :clear @14:52:48.15 Carousel.seek 31 @14:52:48.57 Toroid.in :hold @14:52:48.66 Toroid.seek :cartridge @14:52:50.51 Toroid.in :default @14:52:50.61 Toroid.seek :clear @14:52:53.17 Carousel.seek 32 @14:52:53.69 Toroid.in :hold @14:52:53.79 Toroid.seek :cartridge @14:52:55.69 Toroid.in :default @14:52:55.79 Toroid.seek :clear @14:52:57.96 Carousel.seek 33 @14:52:58.39 Toroid.in :hold @14:52:58.49 Toroid.seek :cartridge @14:53:00.34 Toroid.in :default @14:53:00.44 Toroid.seek :clear @14:53:02.56 Carousel.seek 34 @14:53:02.99 Toroid.in :hold @14:53:03.09 Toroid.seek :cartridge @14:53:04.95 Toroid.in :default @14:53:05.04 Toroid.seek :clear @14:53:07.17 Carousel.seek 35 @14:53:07.59 Toroid.in :hold @14:53:07.72 Toroid.seek :cartridge @14:53:09.58 Toroid.in :default @14:53:09.72 Toroid.seek :clear @14:53:11.88 Carousel.seek 36 @14:53:12.30 Toroid.in :hold @14:53:12.40 Toroid.seek :cartridge @14:53:14.27 Toroid.in :default @14:53:14.36 Toroid.seek :clear @14:53:16.48 Carousel.seek 37 @14:53:16.92 Toroid.in :hold @14:53:17.01 Toroid.seek :cartridge @14:53:18.87 Toroid.in :default @14:53:18.97 Toroid.seek :clear @14:53:21.09 Carousel.seek 38 @14:53:21.52 Toroid.in :hold @14:53:21.64 Toroid.seek :cartridge @14:53:23.50 Toroid.in :default @14:53:23.60 Toroid.seek :clear @14:53:26.29 Carousel.seek 39 @14:53:26.72 Toroid.in :hold @14:53:26.81 Toroid.seek :cartridge @14:53:28.68 Toroid.in :default @14:53:28.77 Toroid.seek :clear @14:53:30.91 Carousel.seek 40 @14:53:31.34 Toroid.in :hold @14:53:31.43 Toroid.seek :cartridge @14:53:33.29 Toroid.in :default @14:53:33.38 Toroid.seek :clear @14:53:35.51 Carousel.seek 41 @14:53:35.93 Toroid.in :hold @14:53:36.08 Toroid.seek :cartridge @14:53:37.94 Toroid.in :default @14:53:38.04 Toroid.seek :clear @14:53:40.18 Carousel.seek 42 @14:53:40.60 Toroid.in :hold @14:53:40.69 Toroid.seek :cartridge @14:53:42.56 Toroid.in :default @14:53:42.66 Toroid.seek :clear @14:53:44.78 Carousel.seek 43 @14:53:45.21 Toroid.in :hold @14:53:45.30 Toroid.seek :cartridge @14:53:47.16 Toroid.in :default @14:53:47.25 Toroid.seek :clear @14:53:49.38 Carousel.seek 44 @14:53:49.81 Toroid.in :hold @14:53:49.90 Toroid.seek :cartridge @14:53:51.76 Toroid.in :default @14:53:51.86 Toroid.seek :clear @14:53:53.99 Carousel.seek 45 @14:53:54.96 Toroid.in :hold @14:53:55.06 Toroid.seek :cartridge @14:53:56.91 Toroid.in :default @14:53:57.00 Toroid.seek :clear @14:53:59.12 Carousel.seek 46 @14:53:59.56 Toroid.in :hold @14:53:59.68 Toroid.seek :cartridge @14:54:01.54 Toroid.in :default @14:54:01.66 Toroid.seek :clear @14:54:03.82 Carousel.seek 47 @14:54:04.25 Toroid.in :hold @14:54:04.35 Toroid.seek :cartridge @14:54:06.21 Toroid.in :default @14:54:06.31 Toroid.seek :clear @14:54:08.43 Carousel.seek 48 @14:54:08.86 Toroid.in :hold @14:54:08.96 Toroid.seek :cartridge @14:54:10.82 Toroid.in :default @14:54:10.92 Toroid.seek :clear @14:54:13.05 Carousel.seek 49 @14:54:13.47 Toroid.in :hold @14:54:13.58 Toroid.seek :cartridge @14:54:15.46 Toroid.in :default @14:54:15.56 Toroid.seek :clear @14:54:17.73 Carousel.seek 50 @14:54:18.16 Toroid.in :hold @14:54:18.26 Toroid.seek :cartridge @14:54:20.12 Toroid.in :default @14:54:20.22 Toroid.seek :clear @14:54:22.34 Carousel.seek 51 @14:54:22.77 Toroid.in :hold @14:54:22.86 Toroid.seek :cartridge @14:54:24.72 Toroid.in :default @14:54:24.81 Toroid.seek :clear @14:54:27.49 Carousel.seek 52 @14:54:27.92 Toroid.in :hold @14:54:28.01 Toroid.seek :cartridge @14:54:29.88 Toroid.in :default @14:54:29.97 Toroid.seek :clear @14:54:32.10 Carousel.seek 53 @14:54:32.52 Toroid.in :hold @14:54:32.61 Toroid.seek :cartridge @14:54:34.48 Toroid.in :default @14:54:34.58 Toroid.seek :clear @14:54:36.70 Carousel.seek 54 @14:54:37.12 Toroid.in :hold @14:54:37.22 Toroid.seek :cartridge @14:54:39.08 Toroid.in :default @14:54:39.17 Toroid.seek :clear @14:54:41.30 Carousel.seek 55 @14:54:41.73 Toroid.in :hold @14:54:41.82 Toroid.seek :cartridge @14:54:43.70 Toroid.in :default @14:54:43.79 Toroid.seek :clear @14:54:45.95 Carousel.seek 56 @14:54:46.38 Toroid.in :hold @14:54:46.48 Toroid.seek :cartridge @14:54:48.33 Toroid.in :default @14:54:48.43 Toroid.seek :clear @14:54:50.56 Carousel.seek 57 @14:54:50.99 Toroid.in :hold @14:54:51.08 Toroid.seek :cartridge @14:54:52.94 Toroid.in :default @14:54:53.04 Toroid.seek :clear @14:54:55.15 Carousel.seek 58 @14:54:55.58 Toroid.in :hold @14:54:56.25 Toroid.seek :cartridge @14:54:58.11 Toroid.in :default @14:54:58.20 Toroid.seek :clear @14:55:00.34 Carousel.seek 59 @14:55:00.75 Toroid.in :hold @14:55:00.86 Toroid.seek :cartridge @14:55:02.71 Toroid.in :default @14:55:02.80 Toroid.seek :clear @14:55:04.95 Carousel.seek 60 @14:55:05.38 Toroid.in :hold @14:55:05.48 Toroid.seek :cartridge @14:55:07.33 Toroid.in :default @14:55:07.43 Toroid.seek :clear @14:55:09.27 Sampling 100.0ml @14:55:09.29 SamplePump.setPosition! 0ml @14:55:09.54 Pressure.in :default @14:55:09.64 Pressure.hold @14:55:09.83 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:55:10.04 SamplePump.in :sample @14:55:10.15 SamplePump.seek 100ml,8:51 @14:59:51.80 SamplePump.hold @14:59:52.03 SamplePump.stop @14:59:52.14 Intake.close @14:59:52.21 Exhaust.close @14:59:52.50 Toroid.in :hold @14:59:52.61 Toroid.seek :bypass @14:59:54.30 Toroid.in :default @14:59:54.42 Toroid.seek :clear @14:59:56.09 Pressure.stop @14:59:56.46 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:02:28.83 -> cleanToroid @15:02:28.86 Cleans Dry SC positions in slots, begin in 10% Bleach @15:02:29.69 Toroid.seek :clear @15:02:29.80 Carousel.in :default @15:02:29.96 Carousel.home.seek 60 @15:02:30.25 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:02:47.94 UserAbort in bill -- User Abort!! @15:03:22.32 -> Cartridge.load! 1..60=>:dry @15:03:22.37 Cartridge::Error in bill -- Toroid must be bypassed before loading new slots @15:03:32.59 -> TV.bypass @15:03:32.73 Toroid.in :hold @15:03:33.38 Toroid.seek :bypass @15:03:35.06 Toroid.in :default @15:03:35.15 Toroid.seek :clear Toroid Valve (TV) clear @15:03:40.11 -> Cartridge.load! 1..60=>:dry @15:03:40.15 Cartridge::Error in bill -- Toroid must be bypassed before loading new slots @15:04:15.67 -> Cartridge.load! 1..60=>:dry @15:04:15.70 Cartridge::Error in bill -- Toroid must be bypassed before loading new slots @15:04:48.19 -> slots {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:05:11.58 -> slots 1..60=>:dry {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:05:17.76 -> cleanToroid @15:05:17.78 Cleans Dry SC positions in slots, begin in 10% Bleach @15:05:18.66 Toroid.seek :clear @15:05:18.77 Carousel.in :default @15:05:18.93 Carousel.home.seek 60 @15:05:19.22 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:05:20.22 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:05:20.33 Priming sample loop w/100ml, bypass w/50ml @15:05:20.35 SamplePump.setPosition! 0ml @15:05:20.44 Bypass.open @15:05:22.04 Intake.open @15:05:23.59 Pressure.in :default @15:05:23.72 Pressure.hold @15:05:23.97 SamplePump.in :prime @15:05:24.07 SamplePump.seek 50ml,2:53 @15:06:25.04 Pressure.stop @15:06:25.12 SamplePump.setPosition! 0ml @15:06:25.20 Exhaust.open @15:06:26.69 Bypass.close @15:06:28.29 Pressure.in :default @15:06:28.38 Pressure.hold @15:06:28.72 SamplePump.in :prime @15:06:28.83 SamplePump.seek 100ml,5:16 @15:07:12.95 SamplePump.hold @15:07:13.20 SamplePump.stop @15:07:13.29 Intake.close @15:07:13.39 Exhaust.close @15:07:14.23 Pressure.stop @15:07:14.34 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:09:15.84 -> cleanToroid @15:09:15.88 Cleans Dry SC positions in slots, begin in 10% Bleach @15:09:17.29 Toroid.seek :clear @15:09:17.41 Carousel.in :default @15:09:17.56 Carousel.home.seek 60 @15:09:17.90 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:09:18.73 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:09:18.85 Priming sample loop w/100ml, bypass w/50ml @15:09:18.87 SamplePump.setPosition! 0ml @15:09:18.96 Bypass.open @15:09:20.56 Intake.open @15:09:22.11 Pressure.in :default @15:09:22.20 Pressure.hold @15:09:22.46 SamplePump.in :prime @15:09:22.57 SamplePump.seek 50ml,2:53 @15:09:45.77 Session Begun @15:09:47.26 -> slots {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:10:23.54 Pressure.stop @15:10:23.67 SamplePump.setPosition! 0ml @15:10:23.76 Exhaust.open @15:10:25.22 Bypass.close @15:10:26.79 Pressure.in :default @15:10:26.91 Pressure.hold @15:10:27.28 SamplePump.in :prime @15:10:27.40 SamplePump.seek 100ml,5:16 @15:10:40.58 SamplePump.hold @15:10:40.85 SamplePump.stop @15:10:40.95 Intake.close @15:10:41.00 Exhaust.close @15:10:41.96 Pressure.stop @15:10:42.08 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:15:55.93 -> slots 1..60=>:dry {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:15:59.72 -> cleanToroid @15:15:59.74 Cleans Dry SC positions in slots, begin in 10% Bleach @15:16:00.64 Toroid.seek :clear @15:16:00.76 Carousel.in :default @15:16:00.93 Carousel.home.seek 60 @15:16:01.27 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:16:02.20 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:16:02.33 Priming sample loop w/100ml, bypass w/50ml @15:16:02.36 SamplePump.setPosition! 0ml @15:16:02.94 Bypass.open @15:16:04.73 Intake.open @15:16:06.29 Pressure.in :default @15:16:06.40 Pressure.hold @15:16:06.69 SamplePump.in :prime @15:16:06.81 SamplePump.seek 50ml,2:53 @15:17:07.80 Pressure.stop @15:17:07.90 SamplePump.setPosition! 0ml @15:17:07.99 Exhaust.open @15:17:09.48 Bypass.close @15:17:11.05 Pressure.in :default @15:17:11.15 Pressure.hold @15:17:11.53 SamplePump.in :prime @15:17:11.67 SamplePump.seek 100ml,5:16 @15:17:29.79 SamplePump.hold @15:17:30.07 SamplePump.stop @15:17:30.19 Intake.close @15:17:30.24 Exhaust.close @15:17:31.13 Pressure.stop @15:17:31.25 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:21:28.72 -> cleanToroid @15:21:28.74 Cleans Dry SC positions in slots, begin in 10% Bleach @15:21:29.67 Toroid.seek :clear @15:21:29.81 Carousel.in :default @15:21:29.98 Carousel.home.seek 60 @15:21:30.32 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:21:32.06 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:21:32.20 Priming sample loop w/100ml, bypass w/50ml @15:21:32.23 SamplePump.setPosition! 0ml @15:21:32.33 Bypass.open @15:21:34.00 Intake.open @15:21:35.55 Pressure.in :default @15:21:35.70 Pressure.hold @15:21:36.00 SamplePump.in :prime @15:21:36.12 SamplePump.seek 50ml,2:53 @15:22:37.12 Pressure.stop @15:22:37.22 SamplePump.setPosition! 0ml @15:22:37.31 Exhaust.open @15:22:38.82 Bypass.close @15:22:41.01 Pressure.in :default @15:22:41.12 Pressure.hold @15:22:41.47 SamplePump.in :prime @15:22:41.61 SamplePump.seek 100ml,5:16 @15:23:09.97 SamplePump.hold @15:23:10.22 SamplePump.stop @15:23:10.34 Intake.close @15:23:10.46 Exhaust.close @15:23:11.32 Pressure.stop @15:23:11.44 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:26:32.95 -> slots 53..55=>:wet {53 => { Type: :microSPR_phyto, State: :wet, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :archiveHiBiomass_bac, State: :wet, TV: :open, was: :dry}, 55 => { Type: :microSPR_phyto, State: :wet, TV: :open, was: :dry}} @15:26:38.52 -> cleanToroid @15:26:38.55 Cleans Dry SC positions in slots, begin in 10% Bleach @15:26:39.42 Toroid.seek :clear @15:26:39.55 Carousel.in :default @15:26:39.76 Carousel.home.seek 60 @15:26:40.08 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:26:47.24 UserAbort in bill -- User Abort!! @15:26:52.36 -> SC.to 53 @15:26:52.42 Carousel.seek 53 Storage Carousel (SC) at 53 @15:26:57.81 -> TV.bypass @15:26:57.99 Toroid.in :hold @15:26:58.10 Toroid.seek :bypass @15:26:59.77 Toroid.in :default @15:26:59.88 Toroid.seek :clear Toroid Valve (TV) clear @15:27:19.53 -> SC.to 54 @15:27:19.57 Carousel.seek 54 Storage Carousel (SC) at 54 @15:27:24.10 -> TV.bypass @15:27:24.26 Toroid.in :hold @15:27:24.38 Toroid.seek :bypass @15:27:26.06 Toroid.in :default @15:27:26.17 Toroid.seek :clear Toroid Valve (TV) clear @15:27:34.90 -> SC.to 55 @15:27:34.93 Carousel.seek 55 Storage Carousel (SC) at 55 @15:27:37.65 -> TV.bypass @15:27:37.81 Toroid.in :hold @15:27:37.92 Toroid.seek :bypass @15:27:39.63 Toroid.in :default @15:27:39.75 Toroid.seek :clear Toroid Valve (TV) clear @15:27:44.98 -> cleanToroid @15:27:45.02 Cleans Dry SC positions in slots, begin in 10% Bleach @15:27:46.55 Toroid.seek :clear @15:27:46.68 Carousel.in :default @15:27:46.86 Carousel.home.seek 55 @15:27:47.18 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:27:48.36 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:27:48.50 Priming sample loop w/100ml, bypass w/50ml @15:27:48.53 SamplePump.setPosition! 0ml @15:27:48.64 Bypass.open @15:27:50.25 Intake.open @15:27:51.80 Pressure.in :default @15:27:51.92 Pressure.hold @15:27:52.21 SamplePump.in :prime @15:27:52.33 SamplePump.seek 50ml,2:53 @15:27:55.31 SamplePump.hold @15:27:55.56 SamplePump.stop @15:27:55.72 Intake.close @15:27:55.78 Bypass.close @15:27:56.69 Pressure.stop @15:27:56.81 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @15:29:55.08 -> cleanToroid @15:29:55.11 Cleans Dry SC positions in slots, begin in 10% Bleach @15:29:56.01 Toroid.seek :clear @15:29:56.14 Carousel.in :default @15:29:56.33 Carousel.home.seek 55 @15:29:56.65 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:29:57.54 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:29:57.72 Priming sample loop w/100ml, bypass w/50ml @15:29:57.75 SamplePump.setPosition! 0ml @15:29:57.86 Bypass.open @15:29:59.49 Intake.open @15:30:01.08 Pressure.in :default @15:30:01.19 Pressure.hold @15:30:01.49 SamplePump.seek 50ml,2:53 @15:31:02.50 Pressure.stop @15:31:02.60 SamplePump.setPosition! 0ml @15:31:02.68 Exhaust.open @15:31:04.17 Bypass.close @15:31:05.79 Pressure.in :default @15:31:05.89 Pressure.hold @15:31:06.89 SamplePump.in :prime @15:31:07.01 SamplePump.seek 100ml,5:16 @15:31:56.41 SamplePump.hold @15:31:56.64 SamplePump.stop @15:31:56.76 Intake.close @15:31:56.86 Exhaust.close @15:31:57.72 Pressure.stop @15:31:57.84 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:32:52.63 -> slots 55=>:dry {55 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:33:06.80 -> TV.cartridge @15:33:06.96 Toroid.in :hold @15:33:07.07 Toroid.seek :cartridge @15:33:08.95 Toroid.in :default @15:33:09.06 Toroid.seek :clear Toroid Valve (TV) clear @15:33:16.63 -> cleanToroid @15:33:16.65 Cleans Dry SC positions in slots, begin in 10% Bleach @15:33:17.57 Toroid.seek :clear @15:33:17.74 Carousel.in :default @15:33:17.91 Carousel.home.seek 55 @15:33:18.23 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:33:19.65 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:33:19.94 Toroid.in :hold @15:33:20.04 Toroid.seek :bypass @15:33:21.73 Toroid.in :default @15:33:21.85 Toroid.seek :clear @15:33:23.50 Priming sample loop w/100ml, bypass w/50ml @15:33:23.53 SamplePump.setPosition! 0ml @15:33:23.67 Bypass.open @15:33:25.28 Intake.open @15:33:26.86 Pressure.in :default @15:33:26.97 Pressure.hold @15:33:27.25 SamplePump.in :prime @15:33:27.38 SamplePump.seek 50ml,2:53 @15:33:54.47 SamplePump.hold @15:33:54.69 SamplePump.stop @15:33:54.80 Intake.close @15:33:54.92 Bypass.close @15:33:55.78 Pressure.stop @15:33:55.89 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @15:34:19.48 -> TV.cartridge @15:34:19.67 Toroid.in :hold @15:34:19.78 Toroid.seek :cartridge @15:34:22.30 Toroid.in :default @15:34:22.43 Toroid.seek :clear Toroid Valve (TV) clear @15:34:27.51 -> SC.to 54 @15:34:27.56 Carousel.seek 54 Storage Carousel (SC) at 54 @15:34:31.14 -> TV.bypass @15:34:31.32 Toroid.in :hold @15:34:31.42 Toroid.seek :bypass @15:34:33.11 Toroid.in :default @15:34:33.22 Toroid.seek :clear Toroid Valve (TV) clear @15:34:38.35 -> cleanToroid @15:34:38.37 Cleans Dry SC positions in slots, begin in 10% Bleach @15:34:39.25 Toroid.seek :clear @15:34:39.37 Carousel.in :default @15:34:39.56 Carousel.home.seek 54 @15:34:39.93 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:34:42.61 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:34:42.74 Priming sample loop w/100ml, bypass w/50ml @15:34:42.76 SamplePump.setPosition! 0ml @15:34:42.88 Bypass.open @15:34:44.51 Intake.open @15:34:46.02 Pressure.in :default @15:34:46.13 Pressure.hold @15:34:46.43 SamplePump.seek 50ml,2:53 @15:35:47.43 Pressure.stop @15:35:47.52 SamplePump.setPosition! 0ml @15:35:47.62 Exhaust.open @15:35:49.16 Bypass.close @15:35:50.78 Pressure.in :default @15:35:50.88 Pressure.hold @15:35:51.24 SamplePump.in :prime @15:35:51.36 SamplePump.seek 100ml,5:16 @15:36:43.23 SamplePump.hold @15:36:43.46 SamplePump.stop @15:36:43.56 Intake.close @15:36:43.72 Exhaust.close @15:36:44.55 Pressure.stop @15:36:44.66 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:39:16.22 -> slots 1..60=>:dry {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, 52..50 => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @15:39:21.39 -> SC.to 54 @15:39:21.44 Carousel.seek 54 Storage Carousel (SC) at 54 @15:39:25.91 -> TV.cartridge @15:39:26.07 Toroid.in :hold @15:39:26.18 Toroid.seek :cartridge @15:39:28.06 Toroid.in :default @15:39:28.17 Toroid.seek :clear Toroid Valve (TV) clear @15:39:33.65 -> SC.to 53 @15:39:33.69 Carousel.seek 53 Storage Carousel (SC) at 53 @15:39:39.03 -> TV.cartridge @15:39:39.20 Toroid.in :hold @15:39:39.32 Toroid.seek :cartridge @15:39:41.19 Toroid.in :default @15:39:41.30 Toroid.seek :clear Toroid Valve (TV) clear @15:39:47.67 -> SC.to 52 @15:39:47.73 Carousel.seek 52 Storage Carousel (SC) at 52 @15:39:53.70 -> cleanToroid @15:39:53.73 Cleans Dry SC positions in slots, begin in 10% Bleach @15:39:54.61 Toroid.seek :clear @15:39:54.73 Carousel.in :default @15:39:54.90 Carousel.home.seek 52 @15:39:55.20 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:39:56.77 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:39:56.93 Toroid.in :hold @15:39:57.05 Toroid.seek :bypass @15:39:58.73 Toroid.in :default @15:39:58.84 Toroid.seek :clear @15:40:00.49 Priming sample loop w/100ml, bypass w/50ml @15:40:00.51 SamplePump.setPosition! 0ml @15:40:00.63 Bypass.open @15:40:02.22 Intake.open @15:40:03.80 Pressure.in :default @15:40:03.92 Pressure.hold @15:40:04.21 SamplePump.in :prime @15:40:04.34 SamplePump.seek 50ml,2:53 @15:40:20.20 SamplePump.hold @15:40:20.43 SamplePump.stop @15:40:20.54 Intake.close @15:40:20.60 Bypass.close @15:40:21.49 Pressure.stop @15:40:21.60 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @15:40:47.16 -> cleanToroid @15:40:47.19 Cleans Dry SC positions in slots, begin in 10% Bleach @15:40:48.77 Toroid.seek :clear @15:40:48.91 Carousel.in :default @15:40:49.10 Carousel.home.seek 52 @15:40:49.42 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:40:50.50 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:40:50.63 Priming sample loop w/100ml, bypass w/50ml @15:40:50.66 SamplePump.setPosition! 0ml @15:40:50.78 Bypass.open @15:40:52.41 Intake.open @15:40:54.01 Pressure.in :default @15:40:54.12 Pressure.hold @15:40:54.42 SamplePump.seek 50ml,2:53 @15:41:33.64 SamplePump.hold @15:41:33.91 SamplePump.stop @15:41:34.02 Intake.close @15:41:34.13 Bypass.close @15:41:34.97 Pressure.stop @15:41:35.10 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @15:44:13.90 -> cleanToroid @15:44:13.93 Cleans Dry SC positions in slots, begin in 10% Bleach @15:44:14.80 Toroid.seek :clear @15:44:14.92 Carousel.in :default @15:44:15.09 Carousel.home.seek 52 @15:44:15.41 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:44:15.94 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:44:16.08 Priming sample loop w/100ml, bypass w/50ml @15:44:16.10 SamplePump.setPosition! 0ml @15:44:16.21 Bypass.open @15:44:17.85 Intake.open @15:44:19.39 Pressure.in :default @15:44:19.51 Pressure.hold @15:44:19.83 SamplePump.seek 50ml,2:53 @15:44:51.97 SamplePump.hold @15:44:52.21 SamplePump.stop @15:44:52.31 Intake.close @15:44:52.38 Bypass.close @15:44:53.26 Pressure.stop @15:44:53.38 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @15:47:16.52 -> cleanToroid @15:47:16.54 Cleans Dry SC positions in slots, begin in 10% Bleach @15:47:18.15 Toroid.seek :clear @15:47:18.27 Carousel.in :default @15:47:18.47 Carousel.home.seek 52 @15:47:18.78 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:47:18.85 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:47:18.98 Priming sample loop w/100ml, bypass w/50ml @15:47:19.01 SamplePump.setPosition! 0ml @15:47:19.11 Bypass.open @15:47:20.72 Intake.open @15:47:22.31 Pressure.in :default @15:47:22.41 Pressure.hold @15:47:22.71 SamplePump.seek 50ml,2:53 @15:48:23.71 Pressure.stop @15:48:23.86 SamplePump.setPosition! 0ml @15:48:23.95 Exhaust.open @15:48:25.43 Bypass.close @15:48:27.02 Pressure.in :default @15:48:27.13 Pressure.hold @15:48:27.49 SamplePump.in :prime @15:48:27.62 SamplePump.seek 100ml,5:16 @15:48:57.96 SamplePump.hold @15:48:58.17 SamplePump.stop @15:48:58.29 Intake.close @15:48:58.39 Exhaust.close @15:48:59.29 Pressure.stop @15:48:59.42 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:53:42.59 -> cleanToroid @15:53:42.62 Cleans Dry SC positions in slots, begin in 10% Bleach @15:53:43.41 Toroid.seek :clear @15:53:43.54 Carousel.in :default @15:53:43.75 Carousel.home.seek 52 @15:53:44.06 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:53:46.62 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:53:46.75 Priming sample loop w/100ml, bypass w/50ml @15:53:46.78 SamplePump.setPosition! 0ml @15:53:46.89 Bypass.open @15:53:48.52 Intake.open @15:53:50.17 Pressure.in :default @15:53:50.28 Pressure.hold @15:53:50.59 SamplePump.in :prime @15:53:50.72 SamplePump.seek 50ml,2:53 @15:54:51.71 Pressure.stop @15:54:51.81 SamplePump.setPosition! 0ml @15:54:51.91 Exhaust.open @15:54:53.37 Bypass.close @15:54:54.99 Pressure.in :default @15:54:55.09 Pressure.hold @15:54:55.45 SamplePump.in :prime @15:54:55.57 SamplePump.seek 100ml,5:16 @15:56:00.56 SamplePump.hold @15:56:00.78 SamplePump.stop @15:56:00.89 Intake.close @15:56:00.99 Exhaust.close @15:56:01.86 Pressure.stop @15:56:01.98 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @15:58:45.12 -> SC.to 53 @15:58:45.18 Carousel.seek 53 Storage Carousel (SC) at 53 @15:58:50.43 -> TV.cartridge @15:58:50.59 Toroid.in :hold @15:58:50.70 Toroid.seek :cartridge @15:58:52.59 Toroid.in :default @15:58:52.70 Toroid.seek :clear Toroid Valve (TV) clear @15:59:00.36 -> Cartridge.lock @15:59:00.58 Lysis1.in :fast @15:59:00.69 Lysis1.seek :locked @15:59:03.36 Plunge.seek :locked @15:59:03.57 Air.in :fast @15:59:03.73 Air.seek :locked @15:59:05.34 Carousel.reconfigure :locked @15:59:05.57 Powering cartridge bus @15:59:08.25 Gate.power :cartridge,:OFF @15:59:08.84 Mounted archive Cartridge @15:59:08.97 Cartridge::Incompatible in bill -- archive sensed when expecting microSPR_phyto in slot 53 @15:59:34.07 -> refillSampleLines @15:59:34.10 Refilling sample & bypass lines with fluid. @15:59:34.99 Toroid.seek :clear @15:59:35.12 Carousel.in :default @15:59:35.99 Carousel.home.seek 53 @15:59:36.47 Carousel.reconfigure :locked @15:59:36.69 Powering cartridge bus @15:59:39.37 Gate.power :cartridge,:OFF @15:59:39.96 Mounted archive Cartridge @15:59:40.06 Cartridge::Incompatible in bill -- archive sensed when expecting microSPR_phyto in slot 53 @16:02:47.72 -> Cartridge.unlock @16:02:47.86 Carousel.in :default @16:02:48.04 Carousel.home.seek 53 @16:02:48.38 Toroid.in :hold @16:02:48.49 Toroid.seek :bypass @16:02:50.17 Toroid.in :default @16:02:50.28 Toroid.seek :clear @16:02:52.10 Air.in :unlocking @16:02:52.31 Air.seek :clear @16:02:53.77 Lysis1.in :unlocking @16:02:53.97 Lysis1.seek :clear @16:02:54.03 Plunge.in :unlocking @16:02:54.22 Plunge.seek :clear @16:06:50.09 -> refillSampleLines @16:06:50.12 Refilling sample & bypass lines with fluid. @16:06:50.97 Toroid.seek :clear @16:06:51.10 Carousel.in :default @16:06:51.28 Carousel.home.seek 53 @16:06:51.58 Waiting for bill to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @16:06:53.46 UserAbort in bill -- User Abort!! @16:06:56.68 -> Cartridge.lock @16:06:56.90 Lysis1.in :fast @16:06:57.01 Lysis1.seek :locked @16:06:59.72 Plunge.in :default @16:06:59.85 Plunge.seek :locked @16:07:00.04 Air.in :fast @16:07:00.14 Air.seek :locked @16:07:01.74 Carousel.reconfigure :locked @16:07:01.95 Powering cartridge bus @16:07:04.63 Gate.power :cartridge,:OFF @16:07:05.22 Mounted archive Cartridge @16:07:05.33 Cartridge::Incompatible in bill -- archive sensed when expecting microSPR_phyto in slot 53 @16:07:09.33 -> refillSampleLines @16:07:09.36 Refilling sample & bypass lines with fluid. @16:07:11.01 Toroid.seek :clear @16:07:11.14 Carousel.in :default @16:07:11.32 Carousel.home.seek 53 @16:07:11.82 Carousel.reconfigure :locked @16:07:12.03 Powering cartridge bus @16:07:14.70 Gate.power :cartridge,:OFF @16:07:15.29 Mounted archive Cartridge @16:07:15.40 Cartridge::Incompatible in bill -- archive sensed when expecting microSPR_phyto in slot 53 @16:07:42.88 -> Cartridge.unlock @16:07:43.01 Carousel.in :default @16:07:43.18 Carousel.home.seek 53 @16:07:43.69 Air.in :unlocking @16:07:43.87 Air.seek :clear @16:07:45.25 Lysis1.in :unlocking @16:07:45.47 Lysis1.seek :clear @16:07:45.62 Plunge.in :unlocking @16:07:45.77 Plunge.seek :clear @16:07:50.48 -> refillSampleLines @16:07:50.52 Refilling sample & bypass lines with fluid. @16:07:51.42 Toroid.seek :clear @16:07:51.54 Carousel.in :default @16:07:51.76 Carousel.home.seek 53 @16:07:52.07 Waiting for bill to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @16:07:52.14 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @16:07:52.16 Bypass.open @16:07:52.95 Intake.open @16:07:53.69 SamplePump.setPosition! 0ml @16:07:53.85 SamplePump.in :default @16:07:53.95 SamplePump.seek 30ml @16:08:02.05 SamplePump.hold @16:08:02.28 delay 1 second @16:08:03.32 Intake.close @16:08:03.42 Bypass.close @16:08:04.27 UserAbort in bill -- Sample Pump (SP) seek to 30ml Aborted! @16:08:07.51 -> TV.cartridge @16:08:07.70 Toroid.in :hold @16:08:07.82 Toroid.seek :cartridge @16:08:09.70 Toroid.in :default @16:08:09.81 Toroid.seek :clear Toroid Valve (TV) clear @16:08:21.94 -> SC.to 52 @16:08:21.99 Carousel.seek 52 Storage Carousel (SC) at 52 @16:08:34.21 -> cleanToroid @16:08:34.23 Cleans Dry SC positions in slots, begin in 10% Bleach @16:08:35.11 Toroid.seek :clear @16:08:35.25 Carousel.in :default @16:08:35.43 Carousel.home.seek 52 @16:08:35.77 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @16:08:35.83 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @16:08:35.96 Priming sample loop w/100ml, bypass w/50ml @16:08:35.99 SamplePump.setPosition! 0ml @16:08:36.09 Bypass.open @16:08:37.66 Intake.open @16:08:38.46 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:08:41.05 Vacuum tare is 56% of full scale @16:08:41.25 Pressure.in :default @16:08:41.37 Pressure.hold @16:08:41.69 SamplePump.in :prime @16:08:41.81 SamplePump.seek 50ml,2:53 @16:09:42.78 Pressure.stop @16:09:42.88 SamplePump.setPosition! 0ml @16:09:42.96 Exhaust.open @16:09:44.44 Bypass.close @16:09:46.04 Pressure.in :default @16:09:46.15 Pressure.hold @16:09:46.51 SamplePump.in :prime @16:09:46.63 SamplePump.seek 100ml,5:16 @16:10:38.67 SamplePump.hold @16:10:38.98 SamplePump.stop @16:10:39.09 Intake.close @16:10:39.21 Exhaust.close @16:10:40.03 Pressure.stop @16:10:40.15 UserAbort in bill -- Sample Pump (SP) seek to 100ml Aborted! @16:10:44.84 -> TV.bypass @16:10:44.99 Toroid.in :hold @16:10:45.11 Toroid.seek :bypass @16:10:46.79 Toroid.in :default @16:10:46.90 Toroid.seek :clear Toroid Valve (TV) clear @16:11:13.65 -> SC.to 54 @16:11:13.69 Carousel.seek 54 Storage Carousel (SC) at 54 @16:11:24.98 -> SC.to 53 @16:11:25.02 Carousel.seek 53 Storage Carousel (SC) at 53 @16:11:30.38 -> TV.bypass @16:11:30.55 Toroid.in :hold @16:11:30.65 Toroid.seek :bypass @16:11:32.34 Toroid.in :default @16:11:32.44 Toroid.seek :clear Toroid Valve (TV) clear @16:17:02.57 -> slots 1..60=>:dry {[59,57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry, TV: :open, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, TV: :open, was: :dry}, [51,50] => { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard}, 52 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}, 60 => { Type: :microSPR_phyto, State: :dry, was: :dry}} @16:17:06.74 -> cleanToroid @16:17:06.76 Cleans Dry SC positions in slots, begin in 10% Bleach @16:17:07.74 Toroid.seek :clear @16:17:07.87 Carousel.in :default @16:17:08.06 Carousel.home.seek 53 @16:17:08.39 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @16:17:10.26 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @16:17:10.39 Priming sample loop w/100ml, bypass w/50ml @16:17:10.42 SamplePump.setPosition! 0ml @16:17:10.53 Bypass.open @16:17:12.05 Intake.open @16:17:12.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:17:15.46 Vacuum tare is 56% of full scale @16:17:15.72 Pressure.in :default @16:17:15.82 Pressure.hold @16:17:16.12 SamplePump.in :prime @16:17:16.23 SamplePump.seek 50ml,2:53 @16:18:17.23 Pressure.stop @16:18:17.33 SamplePump.setPosition! 0ml @16:18:17.42 Exhaust.open @16:18:18.90 Bypass.close @16:18:20.54 Pressure.in :default @16:18:20.64 Pressure.hold @16:18:21.01 SamplePump.in :prime @16:18:21.13 SamplePump.seek 100ml,5:16 @16:21:10.64 Pressure.stop @16:21:10.74 Opening all dry Cartridges @16:21:11.48 Ensuring (60) cartridge(s) open @16:21:11.88 Carousel.seek 52 @16:21:12.33 Toroid.in :hold @16:21:12.44 Toroid.seek :cartridge @16:21:14.33 Toroid.in :default @16:21:14.44 Toroid.seek :clear @16:21:16.59 Carousel.seek 53 @16:21:17.04 Toroid.in :hold @16:21:17.15 Toroid.seek :cartridge @16:21:19.02 Toroid.in :default @16:21:19.13 Toroid.seek :clear @16:21:21.28 Carousel.seek 60 @16:21:22.94 Toroid.in :hold @16:21:23.05 Toroid.seek :cartridge @16:21:24.93 Toroid.in :default @16:21:25.04 Toroid.seek :clear @16:21:26.89 Sampling 100.0ml @16:21:26.91 SamplePump.setPosition! 0ml @16:21:27.19 Pressure.in :default @16:21:27.31 Pressure.hold @16:21:27.50 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @16:21:27.78 SamplePump.in :sample @16:21:27.90 SamplePump.seek 100ml,8:51 @16:28:18.26 Pressure.stop @16:28:18.43 Intake.close @16:28:18.52 Exhaust.close @16:28:18.79 Toroid.in :hold @16:28:18.92 Toroid.seek :bypass @16:28:20.59 Toroid.in :default @16:28:20.70 Toroid.seek :clear @16:28:22.36 Sampled 100.0ml @16:28:22.43 Ensuring (60) cartridge(s) open @16:28:22.80 Carousel.seek 60 @16:28:22.95 Toroid.in :hold @16:28:23.06 Toroid.seek :cartridge @16:28:25.06 Toroid.in :default @16:28:25.17 Toroid.seek :clear @16:28:27.02 Sample loop filled with Bleach. Move intake to clean water source @16:28:27.05 delay 15:00 @16:43:27.20 Toroid.in :hold @16:43:27.32 Toroid.seek :bypass @16:43:29.70 Toroid.in :default @16:43:29.82 Toroid.seek :clear @16:43:31.47 Priming bypass w/50ml @16:43:31.50 SamplePump.setPosition! 0ml @16:43:31.63 Bypass.open @16:43:33.27 Intake.open @16:43:34.84 Pressure.in :default @16:43:34.95 Pressure.hold @16:43:35.29 SamplePump.in :prime @16:43:35.47 SamplePump.seek 50ml,2:53 @16:44:36.53 Pressure.stop @16:44:36.64 Ensuring (60) cartridge(s) open @16:44:36.99 Carousel.seek 60 @16:44:37.16 Toroid.in :hold @16:44:37.27 Toroid.seek :cartridge @16:44:39.14 Toroid.in :default @16:44:39.25 Toroid.seek :clear @16:44:41.10 Sampling 500.0ml @16:44:41.12 SamplePump.setPosition! 0ml @16:44:41.25 Bypass.close @16:44:41.29 Exhaust.open @16:44:42.28 Pressure.in :default @16:44:42.39 Pressure.hold @16:44:42.57 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @16:44:42.80 SamplePump.in :sample @16:44:42.92 SamplePump.seek 500ml,42:11 @16:59:50.28 Flow averaged 0.34ml/s over 15:08 @16:59:50.37 Waiting up to 9 seconds for vacuum to reduce from 16.3 to 8.0psi @16:59:52.73 Relieved excess vacuum in 2.5 seconds @16:59:52.80 SamplePump.seek 500ml,16:46 @16:59:56.27 Flow estimate reduced to 0.27ml/s @16:59:56.36 Waiting up to 11 seconds for vacuum to reduce from 17.3 to 8.0psi @16:59:58.79 Relieved excess vacuum in 2.5 seconds @16:59:58.87 SamplePump.seek 500ml,16:39 @17:00:02.84 Flow estimate reduced to 0.22ml/s @17:00:02.92 Waiting up to 10 seconds for vacuum to reduce from 16.6 to 8.0psi @17:00:05.28 Relieved excess vacuum in 2.4 seconds @17:00:05.35 SamplePump.seek 500ml,16:30 @17:09:26.08 Pressure.stop @17:09:26.27 Intake.close @17:09:26.35 Exhaust.close @17:09:26.65 Toroid.in :hold @17:09:26.76 Toroid.seek :bypass @17:09:28.45 Toroid.in :default @17:09:28.56 Toroid.seek :clear @17:09:30.22 Sampled 500.0ml @17:09:30.30 Ensuring (60) cartridge(s) open @17:09:30.67 Carousel.seek 60 @17:09:30.83 Toroid.in :hold @17:09:30.93 Toroid.seek :cartridge @17:09:32.82 Toroid.in :default @17:09:32.93 Toroid.seek :clear @17:09:34.78 Waiting for bill to answer "Rinse complete. Disconnect intake water source and attach air filter." @17:09:59.39 Session Begun @17:10:05.75 -> TV.cartridge @17:10:05.94 Toroid.in :hold @17:10:06.08 Toroid.seek :cartridge @17:10:07.98 Toroid.in :default @17:10:08.10 Toroid.seek :clear Toroid Valve (TV) clear @17:10:22.30 -> 5.times{refillSampleLines} @17:10:22.34 Refilling sample & bypass lines with fluid. @17:10:23.28 Toroid.seek :clear @17:10:23.43 Carousel.in :default @17:10:23.66 Carousel.home.seek 60 @17:10:24.00 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @17:10:25.64 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @17:10:25.67 Bypass.open @17:10:26.50 Intake.open @17:10:27.21 SamplePump.setPosition! 0ml @17:10:27.40 SamplePump.in :default @17:10:28.27 SamplePump.seek 30ml @17:11:05.16 Bypass.close @17:11:05.96 Exhaust.open @17:11:06.85 SamplePump.seek 55ml @17:11:37.67 delay 1 second @17:11:38.70 Intake.close @17:11:38.76 Exhaust.close @17:11:39.08 Toroid.in :hold @17:11:39.22 Toroid.seek :bypass @17:11:40.92 Toroid.in :default @17:11:41.06 Toroid.seek :clear @17:11:42.73 Sampling Loop filled with water @17:11:42.75 Refilling sample & bypass lines with fluid. @17:11:43.71 Toroid.seek :clear @17:11:43.86 Carousel.in :default @17:11:44.06 Carousel.home.seek 60 @17:11:44.39 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @17:11:44.48 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @17:11:44.50 Bypass.open @17:11:45.38 Intake.open @17:11:46.14 SamplePump.setPosition! 0ml @17:11:46.32 SamplePump.seek 30ml @17:12:23.19 Bypass.close @17:12:23.93 Exhaust.open @17:12:24.81 SamplePump.seek 55ml @17:12:55.65 delay 1 second @17:12:56.68 Intake.close @17:12:56.81 Exhaust.close @17:12:57.68 Sampling Loop filled with water @17:12:57.71 Refilling sample & bypass lines with fluid. @17:12:58.64 Toroid.seek :clear @17:12:58.79 Carousel.in :default @17:12:58.99 Carousel.home.seek 60 @17:12:59.32 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @17:12:59.40 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @17:12:59.41 Bypass.open @17:13:00.22 Intake.open @17:13:00.97 SamplePump.setPosition! 0ml @17:13:01.15 SamplePump.seek 30ml @17:13:38.03 Bypass.close @17:13:38.76 Exhaust.open @17:13:39.59 SamplePump.seek 55ml @17:14:10.45 delay 1 second @17:14:11.49 Intake.close @17:14:11.54 Exhaust.close @17:14:13.31 Sampling Loop filled with water @17:14:13.36 Refilling sample & bypass lines with fluid. @17:14:14.33 Toroid.seek :clear @17:14:14.48 Carousel.in :default @17:14:14.68 Carousel.home.seek 60 @17:14:15.02 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @17:14:15.08 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @17:14:15.11 Bypass.open @17:14:15.94 Intake.open @17:14:16.66 SamplePump.setPosition! 0ml @17:14:16.84 SamplePump.seek 30ml @17:14:53.72 Bypass.close @17:14:54.47 Exhaust.open @17:14:55.33 SamplePump.seek 55ml @17:15:26.15 delay 1 second @17:15:27.20 Intake.close @17:15:27.26 Exhaust.close @17:15:28.11 Sampling Loop filled with water @17:15:28.14 Refilling sample & bypass lines with fluid. @17:15:29.00 Toroid.seek :clear @17:15:29.15 Carousel.in :default @17:15:29.33 Carousel.home.seek 60 @17:15:29.71 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @17:15:29.78 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @17:15:29.80 Bypass.open @17:15:30.60 Intake.open @17:15:31.32 SamplePump.setPosition! 0ml @17:15:31.51 SamplePump.seek 30ml @17:16:08.41 Bypass.close @17:16:09.16 Exhaust.open @17:16:10.01 SamplePump.seek 55ml @17:16:40.84 delay 1 second @17:16:41.87 Intake.close @17:16:42.00 Exhaust.close @17:16:42.87 Sampling Loop filled with water 5 @17:25:00.10 Answered Rinse complete. Disconnect intake water source and attach air filter."" @17:25:00.12 SamplePump.setPosition! 0ml @17:25:00.26 Intake.open @17:25:00.97 Exhaust.open @17:25:01.76 SamplePump.in :slow @17:25:01.89 SamplePump.seek 200ml @17:33:07.63 Intake.close @17:33:08.41 Bypass.open @17:33:09.24 Waiting for bill to answer "Hook up vacuum Flask to to Exhaust line, turn on vacuum, remove Fish in slot 60, push return" @17:54:09.29 Answered Hook up vacuum Flask to to Exhaust line, turn on vacuum, remove Fish in slot 60, push return"" @17:54:09.36 delay 30 seconds @17:54:39.41 Waiting for bill to answer "replace Fish in slot 60, remove fish in slot 1" @17:55:34.89 Answered replace Fish in slot 60, remove fish in slot 1"" @17:55:34.92 delay 30 seconds @17:56:05.05 Waiting for bill to answer "replace Fish in slot 1 and release vacuum" @17:56:35.52 Answered replace Fish in slot 1 and release vacuum"" @17:56:35.56 Ensuring all (59) cartridges are bypassed @17:56:36.04 Carousel.seek 1 @17:56:48.30 Toroid.in :hold @17:56:48.43 Toroid.seek :bypass @17:56:50.12 Toroid.in :default @17:56:50.25 Toroid.seek :clear @17:56:52.22 Carousel.seek 2 @17:56:52.68 Toroid.in :hold @17:56:52.81 Toroid.seek :bypass @17:56:54.50 Toroid.in :default @17:56:54.63 Toroid.seek :clear @17:56:56.61 Carousel.seek 3 @17:56:57.08 Toroid.in :hold @17:56:57.21 Toroid.seek :bypass @17:56:58.90 Toroid.in :default @17:56:59.03 Toroid.seek :clear @17:57:01.00 Carousel.seek 4 @17:57:01.46 Toroid.in :hold @17:57:01.59 Toroid.seek :bypass @17:57:03.34 Toroid.in :default @17:57:03.48 Toroid.seek :clear @17:57:05.44 Carousel.seek 5 @17:57:05.91 Toroid.in :hold @17:57:06.05 Toroid.seek :bypass @17:57:07.74 Toroid.in :default @17:57:07.87 Toroid.seek :clear @17:57:09.88 Carousel.seek 6 @17:57:10.35 Toroid.in :hold @17:57:10.49 Toroid.seek :bypass @17:57:12.21 Toroid.in :default @17:57:12.33 Toroid.seek :clear @17:57:14.98 Carousel.seek 7 @17:57:15.49 Toroid.in :hold @17:57:15.64 Toroid.seek :bypass @17:57:17.34 Toroid.in :default @17:57:17.47 Toroid.seek :clear @17:57:19.44 Carousel.seek 8 @17:57:19.90 Toroid.in :hold @17:57:20.03 Toroid.seek :bypass @17:57:21.72 Toroid.in :default @17:57:21.86 Toroid.seek :clear @17:57:23.85 Carousel.seek 9 @17:57:24.31 Toroid.in :hold @17:57:24.45 Toroid.seek :bypass @17:57:26.14 Toroid.in :default @17:57:26.28 Toroid.seek :clear @17:57:28.25 Carousel.seek 10 @17:57:28.71 Toroid.in :hold @17:57:28.84 Toroid.seek :bypass @17:57:30.53 Toroid.in :default @17:57:30.67 Toroid.seek :clear @17:57:32.65 Carousel.seek 11 @17:57:33.12 Toroid.in :hold @17:57:33.25 Toroid.seek :bypass @17:57:34.94 Toroid.in :default @17:57:35.07 Toroid.seek :clear @17:57:37.03 Carousel.seek 12 @17:57:37.50 Toroid.in :hold @17:57:37.67 Toroid.seek :bypass @17:57:39.37 Toroid.in :default @17:57:39.50 Toroid.seek :clear @17:57:41.48 Carousel.seek 13 @17:57:41.94 Toroid.in :hold @17:57:42.08 Toroid.seek :bypass @17:57:44.40 Toroid.in :default @17:57:44.63 Toroid.seek :clear @17:57:46.62 Carousel.seek 14 @17:57:47.09 Toroid.in :hold @17:57:47.22 Toroid.seek :bypass @17:57:48.92 Toroid.in :default @17:57:49.05 Toroid.seek :clear @17:57:51.03 Carousel.seek 15 @17:57:51.49 Toroid.in :hold @17:57:51.64 Toroid.seek :bypass @17:57:53.35 Toroid.in :default @17:57:53.48 Toroid.seek :clear @17:57:55.44 Carousel.seek 16 @17:57:55.90 Toroid.in :hold @17:57:56.04 Toroid.seek :bypass @17:57:57.74 Toroid.in :default @17:57:57.87 Toroid.seek :clear @17:57:59.86 Carousel.seek 17 @17:58:00.33 Toroid.in :hold @17:58:00.47 Toroid.seek :bypass @17:58:02.16 Toroid.in :default @17:58:02.29 Toroid.seek :clear @17:58:04.25 Carousel.seek 18 @17:58:04.72 Toroid.in :hold @17:58:04.85 Toroid.seek :bypass @17:58:06.55 Toroid.in :default @17:58:06.69 Toroid.seek :clear @17:58:08.65 Carousel.seek 19 @17:58:09.12 Toroid.in :hold @17:58:09.25 Toroid.seek :bypass @17:58:10.94 Toroid.in :default @17:58:11.07 Toroid.seek :clear @17:58:13.79 Carousel.seek 20 @17:58:14.26 Toroid.in :hold @17:58:14.39 Toroid.seek :bypass @17:58:16.06 Toroid.in :default @17:58:16.19 Toroid.seek :clear @17:58:18.16 Carousel.seek 21 @17:58:18.62 Toroid.in :hold @17:58:18.75 Toroid.seek :bypass @17:58:20.45 Toroid.in :default @17:58:20.58 Toroid.seek :clear @17:58:22.56 Carousel.seek 22 @17:58:23.02 Toroid.in :hold @17:58:23.15 Toroid.seek :bypass @17:58:24.85 Toroid.in :default @17:58:24.98 Toroid.seek :clear @17:58:26.95 Carousel.seek 23 @17:58:27.41 Toroid.in :hold @17:58:27.54 Toroid.seek :bypass @17:58:29.30 Toroid.in :default @17:58:29.43 Toroid.seek :clear @17:58:31.39 Carousel.seek 24 @17:58:31.86 Toroid.in :hold @17:58:31.99 Toroid.seek :bypass @17:58:33.70 Toroid.in :default @17:58:33.83 Toroid.seek :clear @17:58:35.85 Carousel.seek 25 @17:58:36.31 Toroid.in :hold @17:58:36.44 Toroid.seek :bypass @17:58:38.14 Toroid.in :default @17:58:38.27 Toroid.seek :clear @17:58:40.22 Carousel.seek 26 @17:58:40.64 Toroid.in :hold @17:58:40.77 Toroid.seek :bypass @17:58:43.26 Toroid.in :default @17:58:43.39 Toroid.seek :clear @17:58:45.36 Carousel.seek 27 @17:58:45.82 Toroid.in :hold @17:58:45.98 Toroid.seek :bypass @17:58:47.70 Toroid.in :default @17:58:47.83 Toroid.seek :clear @17:58:49.83 Carousel.seek 28 @17:58:50.29 Toroid.in :hold @17:58:50.43 Toroid.seek :bypass @17:58:52.12 Toroid.in :default @17:58:52.25 Toroid.seek :clear @17:58:54.23 Carousel.seek 29 @17:58:54.70 Toroid.in :hold @17:58:54.83 Toroid.seek :bypass @17:58:56.52 Toroid.in :default @17:58:56.66 Toroid.seek :clear @17:58:58.64 Carousel.seek 30 @17:58:59.10 Toroid.in :hold @17:58:59.23 Toroid.seek :bypass @17:59:00.92 Toroid.in :default @17:59:01.05 Toroid.seek :clear @17:59:03.01 Carousel.seek 31 @17:59:03.48 Toroid.in :hold @17:59:03.63 Toroid.seek :bypass @17:59:05.35 Toroid.in :default @17:59:05.48 Toroid.seek :clear @17:59:07.44 Carousel.seek 32 @17:59:07.91 Toroid.in :hold @17:59:08.04 Toroid.seek :bypass @17:59:09.74 Toroid.in :default @17:59:09.87 Toroid.seek :clear @17:59:12.60 Carousel.seek 33 @17:59:13.07 Toroid.in :hold @17:59:13.21 Toroid.seek :bypass @17:59:14.89 Toroid.in :default @17:59:15.02 Toroid.seek :clear @17:59:16.98 Carousel.seek 34 @17:59:17.45 Toroid.in :hold @17:59:17.66 Toroid.seek :bypass @17:59:19.43 Toroid.in :default @17:59:19.57 Toroid.seek :clear @17:59:21.58 Carousel.seek 35 @17:59:22.08 Toroid.in :hold @17:59:22.22 Toroid.seek :bypass @17:59:23.90 Toroid.in :default @17:59:24.03 Toroid.seek :clear @17:59:25.99 Carousel.seek 36 @17:59:26.46 Toroid.in :hold @17:59:26.59 Toroid.seek :bypass @17:59:28.28 Toroid.in :default @17:59:28.41 Toroid.seek :clear @17:59:30.37 Carousel.seek 37 @17:59:30.84 Toroid.in :hold @17:59:30.97 Toroid.seek :bypass @17:59:32.67 Toroid.in :default @17:59:32.80 Toroid.seek :clear @17:59:34.77 Carousel.seek 38 @17:59:35.25 Toroid.in :hold @17:59:35.38 Toroid.seek :bypass @17:59:37.08 Toroid.in :default @17:59:37.21 Toroid.seek :clear @17:59:39.18 Carousel.seek 39 @17:59:39.68 Toroid.in :hold @17:59:40.55 Toroid.seek :bypass @17:59:42.24 Toroid.in :default @17:59:42.37 Toroid.seek :clear @17:59:44.34 Carousel.seek 40 @17:59:44.81 Toroid.in :hold @17:59:44.94 Toroid.seek :bypass @17:59:46.63 Toroid.in :default @17:59:46.76 Toroid.seek :clear @17:59:48.72 Carousel.seek 41 @17:59:49.19 Toroid.in :hold @17:59:49.32 Toroid.seek :bypass @17:59:51.01 Toroid.in :default @17:59:51.14 Toroid.seek :clear @17:59:53.11 Carousel.seek 42 @17:59:53.58 Toroid.in :hold @17:59:53.73 Toroid.seek :bypass @17:59:55.43 Toroid.in :default @17:59:55.56 Toroid.seek :clear @17:59:57.57 Carousel.seek 43 @17:59:58.09 Toroid.in :hold @17:59:58.22 Toroid.seek :bypass @17:59:59.92 Toroid.in :default @18:00:00.04 Toroid.seek :clear @18:00:02.00 Carousel.seek 44 @18:00:02.47 Toroid.in :hold @18:00:02.60 Toroid.seek :bypass @18:00:04.29 Toroid.in :default @18:00:04.42 Toroid.seek :clear @18:00:06.40 Carousel.seek 45 @18:00:06.87 Toroid.in :hold @18:00:07.00 Toroid.seek :bypass @18:00:08.69 Toroid.in :default @18:00:08.81 Toroid.seek :clear @18:00:10.88 Carousel.seek 46 @18:00:11.35 Toroid.in :hold @18:00:11.49 Toroid.seek :bypass @18:00:13.18 Toroid.in :default @18:00:13.32 Toroid.seek :clear @18:00:15.30 Carousel.seek 47 @18:00:15.77 Toroid.in :hold @18:00:15.90 Toroid.seek :bypass @18:00:17.58 Toroid.in :default @18:00:17.74 Toroid.seek :clear @18:00:19.74 Carousel.seek 48 @18:00:20.21 Toroid.in :hold @18:00:20.34 Toroid.seek :bypass @18:00:22.04 Toroid.in :default @18:00:22.17 Toroid.seek :clear @18:00:24.14 Carousel.seek 49 @18:00:24.61 Toroid.in :hold @18:00:24.74 Toroid.seek :bypass @18:00:26.43 Toroid.in :default @18:00:26.56 Toroid.seek :clear @18:00:28.52 Carousel.seek 50 @18:00:28.99 Toroid.in :hold @18:00:29.12 Toroid.seek :bypass @18:00:30.81 Toroid.in :default @18:00:30.95 Toroid.seek :clear @18:00:32.93 Carousel.seek 51 @18:00:33.40 Toroid.in :hold @18:00:33.53 Toroid.seek :bypass @18:00:35.26 Toroid.in :default @18:00:35.38 Toroid.seek :clear @18:00:37.35 Carousel.seek 52 @18:00:38.57 Toroid.in :hold @18:00:38.71 Toroid.seek :bypass @18:00:40.39 Toroid.in :default @18:00:40.52 Toroid.seek :clear @18:00:42.49 Carousel.seek 53 @18:00:42.95 Toroid.in :hold @18:00:43.08 Toroid.seek :bypass @18:00:44.77 Toroid.in :default @18:00:44.91 Toroid.seek :clear @18:00:46.88 Carousel.seek 54 @18:00:47.35 Toroid.in :hold @18:00:47.48 Toroid.seek :bypass @18:00:49.17 Toroid.in :default @18:00:49.29 Toroid.seek :clear @18:00:51.25 Carousel.seek 55 @18:00:51.71 Toroid.in :hold @18:00:51.85 Toroid.seek :bypass @18:00:53.54 Toroid.in :default @18:00:53.70 Toroid.seek :clear @18:00:55.69 Carousel.seek 56 @18:00:56.15 Toroid.in :hold @18:00:56.29 Toroid.seek :bypass @18:00:57.98 Toroid.in :default @18:00:58.10 Toroid.seek :clear @18:01:00.08 Carousel.seek 57 @18:01:00.54 Toroid.in :hold @18:01:00.68 Toroid.seek :bypass @18:01:02.36 Toroid.in :default @18:01:02.49 Toroid.seek :clear @18:01:04.45 Carousel.seek 58 @18:01:04.92 Toroid.in :hold @18:01:05.06 Toroid.seek :bypass @18:01:06.74 Toroid.in :default @18:01:07.52 Toroid.seek :clear @18:01:09.67 Carousel.seek 59 @18:01:10.13 Toroid.in :hold @18:01:10.26 Toroid.seek :bypass @18:01:11.95 Toroid.in :default @18:01:12.08 Toroid.seek :clear @18:01:13.75 All dry cartridges bypassed. Sample loop and cartridge ports cleaned and dry. @18:01:13.76 Ready to load cartridges. After cartridges loaded run vacLeakTest @18:06:51.35 -> SC.to 35 @18:06:51.39 Carousel.seek 35 Storage Carousel (SC) at 35 @18:11:41.55 -> SC.to 10 @18:11:41.58 Carousel.seek 10 Storage Carousel (SC) at 10 @18:14:04.50 -> vacToroid @18:14:04.60 Vacuuming protocol for dry cartridges @18:14:05.46 Toroid.seek :clear @18:14:05.63 Carousel.in :default @18:14:05.85 Carousel.home.seek 10 @18:14:06.20 Waiting for bill to answer "Disconnect the intake line. push return" @18:14:11.36 Answered Disconnect the intake line. push return"" @18:14:12.24 Toroid.seek :clear @18:14:12.38 Carousel.in :default @18:14:12.56 Carousel.home.seek 10 @18:14:12.89 SamplePump.setPosition! 0ml @18:14:13.00 Intake.open @18:14:13.73 SamplePump.in :default @18:14:13.85 SamplePump.seek 50ml @18:15:15.03 Exhaust.close @18:15:15.81 SamplePump.seek 75ml @18:15:46.64 Intake.close @18:15:47.38 Bypass.close @18:15:48.13 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @18:15:57.04 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @18:15:57.05 Exhaust.open @18:15:57.89 Bypass.open @18:15:58.66 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @18:16:02.38 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @18:16:02.40 delay 30 seconds @18:16:32.49 Clearing fluid from cartridge-side of Toroid @18:16:32.85 Carousel.seek 1 @18:16:34.96 Toroid.in :hold @18:16:35.08 Toroid.seek :cartridge @18:16:36.97 Toroid.in :default @18:16:37.09 Toroid.seek :clear @18:16:39.71 delay 2 seconds @18:16:41.83 Toroid.in :hold @18:16:41.96 Toroid.seek :bypass @18:16:43.69 Toroid.in :default @18:16:43.82 Toroid.seek :clear @18:16:45.83 Carousel.seek 2 @18:16:46.31 Toroid.in :hold @18:16:46.44 Toroid.seek :cartridge @18:16:48.33 Toroid.in :default @18:16:48.45 Toroid.seek :clear @18:16:50.32 delay 2 seconds @18:16:52.44 Toroid.in :hold @18:16:52.56 Toroid.seek :bypass @18:16:54.25 Toroid.in :default @18:16:54.38 Toroid.seek :clear @18:16:56.35 Carousel.seek 3 @18:16:56.81 Toroid.in :hold @18:16:56.94 Toroid.seek :cartridge @18:16:58.84 Toroid.in :default @18:16:58.96 Toroid.seek :clear @18:17:00.83 delay 2 seconds @18:17:02.96 Toroid.in :hold @18:17:03.08 Toroid.seek :bypass @18:17:04.78 Toroid.in :default @18:17:04.92 Toroid.seek :clear @18:17:06.88 Carousel.seek 4 @18:17:07.34 Toroid.in :hold @18:17:07.47 Toroid.seek :cartridge @18:17:09.36 Toroid.in :default @18:17:09.49 Toroid.seek :clear @18:17:11.35 delay 2 seconds @18:17:13.47 Toroid.in :hold @18:17:13.59 Toroid.seek :bypass @18:17:15.33 Toroid.in :default @18:17:15.46 Toroid.seek :clear @18:17:17.42 Carousel.seek 5 @18:17:17.89 Toroid.in :hold @18:17:18.02 Toroid.seek :cartridge @18:17:19.90 Toroid.in :default @18:17:20.04 Toroid.seek :clear @18:17:22.53 delay 2 seconds @18:17:24.75 Toroid.in :hold @18:17:24.88 Toroid.seek :bypass @18:17:26.57 Toroid.in :default @18:17:26.70 Toroid.seek :clear @18:17:28.68 Carousel.seek 6 @18:17:29.14 Toroid.in :hold @18:17:29.28 Toroid.seek :cartridge @18:17:31.17 Toroid.in :default @18:17:31.30 Toroid.seek :clear @18:17:33.16 delay 2 seconds @18:17:35.28 Toroid.in :hold @18:17:35.42 Toroid.seek :bypass @18:17:37.12 Toroid.in :default @18:17:37.24 Toroid.seek :clear @18:17:39.21 Carousel.seek 7 @18:17:39.70 Toroid.in :hold @18:17:39.83 Toroid.seek :cartridge @18:17:41.72 Toroid.in :default @18:17:41.85 Toroid.seek :clear @18:17:43.71 delay 2 seconds @18:17:45.84 Toroid.in :hold @18:17:45.98 Toroid.seek :bypass @18:17:47.70 Toroid.in :default @18:17:47.83 Toroid.seek :clear @18:17:49.83 Carousel.seek 8 @18:17:50.29 Toroid.in :hold @18:17:50.42 Toroid.seek :cartridge @18:17:52.30 Toroid.in :default @18:17:52.43 Toroid.seek :clear @18:17:54.30 delay 2 seconds @18:17:56.42 Toroid.in :hold @18:17:56.55 Toroid.seek :bypass @18:17:58.26 Toroid.in :default @18:17:58.38 Toroid.seek :clear @18:18:00.33 Carousel.seek 9 @18:18:00.80 Toroid.in :hold @18:18:00.93 Toroid.seek :cartridge @18:18:02.83 Toroid.in :default @18:18:02.96 Toroid.seek :clear @18:18:04.83 delay 2 seconds @18:18:07.06 Toroid.in :hold @18:18:07.19 Toroid.seek :bypass @18:18:08.89 Toroid.in :default @18:18:09.02 Toroid.seek :clear @18:18:10.99 Carousel.seek 10 @18:18:11.46 Toroid.in :hold @18:18:11.60 Toroid.seek :cartridge @18:18:13.52 Toroid.in :default @18:18:13.69 Toroid.seek :clear @18:18:15.55 delay 2 seconds @18:18:17.70 Toroid.in :hold @18:18:17.84 Toroid.seek :bypass @18:18:19.53 Toroid.in :default @18:18:19.69 Toroid.seek :clear @18:18:21.70 Carousel.seek 11 @18:18:22.16 Toroid.in :hold @18:18:22.30 Toroid.seek :cartridge @18:18:24.19 Toroid.in :default @18:18:24.32 Toroid.seek :clear @18:18:26.19 delay 2 seconds @18:18:28.31 Toroid.in :hold @18:18:28.44 Toroid.seek :bypass @18:18:30.14 Toroid.in :default @18:18:30.26 Toroid.seek :clear @18:18:32.23 Carousel.seek 12 @18:18:32.71 Toroid.in :hold @18:18:32.83 Toroid.seek :cartridge @18:18:34.73 Toroid.in :default @18:18:34.85 Toroid.seek :clear @18:18:36.71 delay 2 seconds @18:18:38.83 Toroid.in :hold @18:18:38.95 Toroid.seek :bypass @18:18:40.64 Toroid.in :default @18:18:40.77 Toroid.seek :clear @18:18:42.73 Carousel.seek 13 @18:18:43.19 Toroid.in :hold @18:18:43.33 Toroid.seek :cartridge @18:18:45.22 Toroid.in :default @18:18:45.35 Toroid.seek :clear @18:18:47.20 delay 2 seconds @18:18:50.07 Toroid.in :hold @18:18:50.21 Toroid.seek :bypass @18:18:51.89 Toroid.in :default @18:18:52.03 Toroid.seek :clear @18:18:53.99 Carousel.seek 14 @18:18:54.45 Toroid.in :hold @18:18:54.59 Toroid.seek :cartridge @18:18:56.47 Toroid.in :default @18:18:56.60 Toroid.seek :clear @18:18:58.46 delay 2 seconds @18:19:00.58 Toroid.in :hold @18:19:00.71 Toroid.seek :bypass @18:19:02.41 Toroid.in :default @18:19:02.55 Toroid.seek :clear @18:19:04.53 Carousel.seek 15 @18:19:05.00 Toroid.in :hold @18:19:05.13 Toroid.seek :cartridge @18:19:07.02 Toroid.in :default @18:19:07.15 Toroid.seek :clear @18:19:09.01 delay 2 seconds @18:19:11.13 Toroid.in :hold @18:19:11.26 Toroid.seek :bypass @18:19:12.95 Toroid.in :default @18:19:13.08 Toroid.seek :clear @18:19:15.05 Carousel.seek 16 @18:19:15.51 Toroid.in :hold @18:19:15.69 Toroid.seek :cartridge @18:19:17.58 Toroid.in :default @18:19:17.74 Toroid.seek :clear @18:19:19.60 delay 2 seconds @18:19:21.74 Toroid.in :hold @18:19:21.88 Toroid.seek :bypass @18:19:23.58 Toroid.in :default @18:19:23.74 Toroid.seek :clear @18:19:25.74 Carousel.seek 17 @18:19:26.20 Toroid.in :hold @18:19:26.34 Toroid.seek :cartridge @18:19:28.22 Toroid.in :default @18:19:28.36 Toroid.seek :clear @18:19:30.20 delay 2 seconds @18:19:33.05 Toroid.in :hold @18:19:33.18 Toroid.seek :bypass @18:19:34.89 Toroid.in :default @18:19:35.04 Toroid.seek :clear @18:19:37.01 Carousel.seek 18 @18:19:37.48 Toroid.in :hold @18:19:37.63 Toroid.seek :cartridge @18:19:39.52 Toroid.in :default @18:19:39.69 Toroid.seek :clear @18:19:41.56 delay 2 seconds @18:19:43.71 Toroid.in :hold @18:19:43.85 Toroid.seek :bypass @18:19:45.53 Toroid.in :default @18:19:45.70 Toroid.seek :clear @18:19:47.68 Carousel.seek 19 @18:19:48.15 Toroid.in :hold @18:19:48.28 Toroid.seek :cartridge @18:19:50.17 Toroid.in :default @18:19:50.31 Toroid.seek :clear @18:19:52.18 delay 2 seconds @18:19:54.30 Toroid.in :hold @18:19:54.43 Toroid.seek :bypass @18:19:56.14 Toroid.in :default @18:19:56.27 Toroid.seek :clear @18:19:58.24 Carousel.seek 20 @18:19:58.72 Toroid.in :hold @18:19:58.85 Toroid.seek :cartridge @18:20:00.73 Toroid.in :default @18:20:00.87 Toroid.seek :clear @18:20:02.72 delay 2 seconds @18:20:04.84 Toroid.in :hold @18:20:04.98 Toroid.seek :bypass @18:20:06.69 Toroid.in :default @18:20:06.82 Toroid.seek :clear @18:20:08.78 Carousel.seek 21 @18:20:09.25 Toroid.in :hold @18:20:09.39 Toroid.seek :cartridge @18:20:11.28 Toroid.in :default @18:20:11.41 Toroid.seek :clear @18:20:13.28 delay 2 seconds @18:20:16.08 Toroid.in :hold @18:20:16.30 Toroid.seek :bypass @18:20:18.01 Toroid.in :default @18:20:18.14 Toroid.seek :clear @18:20:20.11 Carousel.seek 22 @18:20:20.58 Toroid.in :hold @18:20:20.72 Toroid.seek :cartridge @18:20:22.60 Toroid.in :default @18:20:22.73 Toroid.seek :clear @18:20:24.59 delay 2 seconds @18:20:26.71 Toroid.in :hold @18:20:26.84 Toroid.seek :bypass @18:20:28.55 Toroid.in :default @18:20:28.67 Toroid.seek :clear @18:20:30.65 Carousel.seek 23 @18:20:31.13 Toroid.in :hold @18:20:31.26 Toroid.seek :cartridge @18:20:33.14 Toroid.in :default @18:20:33.27 Toroid.seek :clear @18:20:35.13 delay 2 seconds @18:20:37.25 Toroid.in :hold @18:20:37.38 Toroid.seek :bypass @18:20:39.08 Toroid.in :default @18:20:39.20 Toroid.seek :clear @18:20:41.17 Carousel.seek 24 @18:20:41.66 Toroid.in :hold @18:20:41.81 Toroid.seek :cartridge @18:20:43.71 Toroid.in :default @18:20:43.83 Toroid.seek :clear @18:20:45.70 delay 2 seconds @18:20:47.81 Toroid.in :hold @18:20:47.95 Toroid.seek :bypass @18:20:49.63 Toroid.in :default @18:20:49.77 Toroid.seek :clear @18:20:51.77 Carousel.seek 25 @18:20:52.23 Toroid.in :hold @18:20:52.36 Toroid.seek :cartridge @18:20:54.24 Toroid.in :default @18:20:54.37 Toroid.seek :clear @18:20:56.23 delay 2 seconds @18:20:58.97 Toroid.in :hold @18:20:59.20 Toroid.seek :bypass @18:21:00.89 Toroid.in :default @18:21:01.03 Toroid.seek :clear @18:21:03.00 Carousel.seek 26 @18:21:03.47 Toroid.in :hold @18:21:03.61 Toroid.seek :cartridge @18:21:05.54 Toroid.in :default @18:21:05.70 Toroid.seek :clear @18:21:07.56 delay 2 seconds @18:21:09.72 Toroid.in :hold @18:21:09.85 Toroid.seek :bypass @18:21:11.55 Toroid.in :default @18:21:11.71 Toroid.seek :clear @18:21:13.72 Carousel.seek 27 @18:21:14.18 Toroid.in :hold @18:21:14.31 Toroid.seek :cartridge @18:21:16.20 Toroid.in :default @18:21:16.33 Toroid.seek :clear @18:21:18.19 delay 2 seconds @18:21:20.31 Toroid.in :hold @18:21:20.44 Toroid.seek :bypass @18:21:22.14 Toroid.in :default @18:21:22.27 Toroid.seek :clear @18:21:24.24 Carousel.seek 28 @18:21:24.70 Toroid.in :hold @18:21:24.83 Toroid.seek :cartridge @18:21:26.71 Toroid.in :default @18:21:26.84 Toroid.seek :clear @18:21:28.71 delay 2 seconds @18:21:30.83 Toroid.in :hold @18:21:30.97 Toroid.seek :bypass @18:21:32.66 Toroid.in :default @18:21:32.79 Toroid.seek :clear @18:21:34.74 Carousel.seek 29 @18:21:35.21 Toroid.in :hold @18:21:35.34 Toroid.seek :cartridge @18:21:37.24 Toroid.in :default @18:21:37.36 Toroid.seek :clear @18:21:39.23 delay 2 seconds @18:21:41.35 Toroid.in :hold @18:21:42.23 Toroid.seek :bypass @18:21:43.92 Toroid.in :default @18:21:44.06 Toroid.seek :clear @18:21:46.04 Carousel.seek 30 @18:21:46.51 Toroid.in :hold @18:21:46.64 Toroid.seek :cartridge @18:21:48.52 Toroid.in :default @18:21:48.65 Toroid.seek :clear @18:21:50.51 delay 2 seconds @18:21:52.63 Toroid.in :hold @18:21:52.78 Toroid.seek :bypass @18:21:54.46 Toroid.in :default @18:21:54.59 Toroid.seek :clear @18:21:56.55 Carousel.seek 31 @18:21:56.99 Toroid.in :hold @18:21:57.13 Toroid.seek :cartridge @18:21:59.01 Toroid.in :default @18:21:59.14 Toroid.seek :clear @18:22:01.00 delay 2 seconds @18:22:03.12 Toroid.in :hold @18:22:03.25 Toroid.seek :bypass @18:22:04.94 Toroid.in :default @18:22:05.07 Toroid.seek :clear @18:22:07.05 Carousel.seek 32 @18:22:07.52 Toroid.in :hold @18:22:07.68 Toroid.seek :cartridge @18:22:09.58 Toroid.in :default @18:22:09.75 Toroid.seek :clear @18:22:11.66 delay 2 seconds @18:22:13.78 Toroid.in :hold @18:22:13.91 Toroid.seek :bypass @18:22:15.62 Toroid.in :default @18:22:15.78 Toroid.seek :clear @18:22:17.76 Carousel.seek 33 @18:22:18.23 Toroid.in :hold @18:22:18.36 Toroid.seek :cartridge @18:22:20.25 Toroid.in :default @18:22:20.38 Toroid.seek :clear @18:22:22.25 delay 2 seconds @18:22:24.37 Toroid.in :hold @18:22:25.23 Toroid.seek :bypass @18:22:26.92 Toroid.in :default @18:22:27.06 Toroid.seek :clear @18:22:29.03 Carousel.seek 34 @18:22:29.49 Toroid.in :hold @18:22:29.65 Toroid.seek :cartridge @18:22:31.54 Toroid.in :default @18:22:31.70 Toroid.seek :clear @18:22:33.56 delay 2 seconds @18:22:35.71 Toroid.in :hold @18:22:35.84 Toroid.seek :bypass @18:22:37.55 Toroid.in :default @18:22:37.72 Toroid.seek :clear @18:22:39.71 Carousel.seek 35 @18:22:40.18 Toroid.in :hold @18:22:40.31 Toroid.seek :cartridge @18:22:42.23 Toroid.in :default @18:22:42.36 Toroid.seek :clear @18:22:44.23 delay 2 seconds @18:22:46.35 Toroid.in :hold @18:22:46.48 Toroid.seek :bypass @18:22:48.18 Toroid.in :default @18:22:48.31 Toroid.seek :clear @18:22:50.27 Carousel.seek 36 @18:22:50.73 Toroid.in :hold @18:22:50.86 Toroid.seek :cartridge @18:22:52.75 Toroid.in :default @18:22:52.93 Toroid.seek :clear @18:22:54.85 delay 2 seconds @18:22:56.99 Toroid.in :hold @18:22:57.12 Toroid.seek :bypass @18:22:58.82 Toroid.in :default @18:22:58.95 Toroid.seek :clear @18:23:00.91 Carousel.seek 37 @18:23:01.38 Toroid.in :hold @18:23:01.51 Toroid.seek :cartridge @18:23:03.40 Toroid.in :default @18:23:03.52 Toroid.seek :clear @18:23:05.41 delay 2 seconds @18:23:07.53 Toroid.in :hold @18:23:08.33 Toroid.seek :bypass @18:23:10.13 Toroid.in :default @18:23:10.26 Toroid.seek :clear @18:23:12.22 Carousel.seek 38 @18:23:12.69 Toroid.in :hold @18:23:12.82 Toroid.seek :cartridge @18:23:14.72 Toroid.in :default @18:23:14.85 Toroid.seek :clear @18:23:16.71 delay 2 seconds @18:23:18.84 Toroid.in :hold @18:23:18.97 Toroid.seek :bypass @18:23:20.67 Toroid.in :default @18:23:20.81 Toroid.seek :clear @18:23:22.77 Carousel.seek 39 @18:23:23.24 Toroid.in :hold @18:23:23.38 Toroid.seek :cartridge @18:23:25.27 Toroid.in :default @18:23:25.41 Toroid.seek :clear @18:23:27.27 delay 2 seconds @18:23:29.39 Toroid.in :hold @18:23:29.52 Toroid.seek :bypass @18:23:31.26 Toroid.in :default @18:23:31.38 Toroid.seek :clear @18:23:33.35 Carousel.seek 40 @18:23:33.81 Toroid.in :hold @18:23:33.95 Toroid.seek :cartridge @18:23:35.84 Toroid.in :default @18:23:35.97 Toroid.seek :clear @18:23:37.83 delay 2 seconds @18:23:39.96 Toroid.in :hold @18:23:40.08 Toroid.seek :bypass @18:23:41.77 Toroid.in :default @18:23:41.90 Toroid.seek :clear @18:23:43.91 Carousel.seek 41 @18:23:44.37 Toroid.in :hold @18:23:44.51 Toroid.seek :cartridge @18:23:46.39 Toroid.in :default @18:23:46.52 Toroid.seek :clear @18:23:48.39 delay 2 seconds @18:23:50.51 Toroid.in :hold @18:23:51.29 Toroid.seek :bypass @18:23:53.09 Toroid.in :default @18:23:53.23 Toroid.seek :clear @18:23:55.19 Carousel.seek 42 @18:23:55.69 Toroid.in :hold @18:23:55.83 Toroid.seek :cartridge @18:23:57.72 Toroid.in :default @18:23:57.85 Toroid.seek :clear @18:23:59.71 delay 2 seconds @18:24:01.83 Toroid.in :hold @18:24:01.96 Toroid.seek :bypass @18:24:03.69 Toroid.in :default @18:24:03.81 Toroid.seek :clear @18:24:05.82 Carousel.seek 43 @18:24:06.29 Toroid.in :hold @18:24:06.42 Toroid.seek :cartridge @18:24:08.31 Toroid.in :default @18:24:08.45 Toroid.seek :clear @18:24:10.30 delay 2 seconds @18:24:12.41 Toroid.in :hold @18:24:12.55 Toroid.seek :bypass @18:24:14.26 Toroid.in :default @18:24:14.39 Toroid.seek :clear @18:24:16.35 Carousel.seek 44 @18:24:16.82 Toroid.in :hold @18:24:16.96 Toroid.seek :cartridge @18:24:18.84 Toroid.in :default @18:24:18.97 Toroid.seek :clear @18:24:20.83 delay 2 seconds @18:24:22.95 Toroid.in :hold @18:24:23.08 Toroid.seek :bypass @18:24:24.78 Toroid.in :default @18:24:24.91 Toroid.seek :clear @18:24:26.86 Carousel.seek 45 @18:24:27.32 Toroid.in :hold @18:24:27.46 Toroid.seek :cartridge @18:24:29.34 Toroid.in :default @18:24:29.47 Toroid.seek :clear @18:24:31.34 delay 2 seconds @18:24:33.46 Toroid.in :hold @18:24:33.60 Toroid.seek :bypass @18:24:36.12 Toroid.in :default @18:24:36.25 Toroid.seek :clear @18:24:38.22 Carousel.seek 46 @18:24:38.68 Toroid.in :hold @18:24:38.82 Toroid.seek :cartridge @18:24:40.70 Toroid.in :default @18:24:40.84 Toroid.seek :clear @18:24:42.70 delay 2 seconds @18:24:44.82 Toroid.in :hold @18:24:44.95 Toroid.seek :bypass @18:24:46.66 Toroid.in :default @18:24:46.78 Toroid.seek :clear @18:24:48.74 Carousel.seek 47 @18:24:49.21 Toroid.in :hold @18:24:49.34 Toroid.seek :cartridge @18:24:51.22 Toroid.in :default @18:24:51.36 Toroid.seek :clear @18:24:53.22 delay 2 seconds @18:24:55.34 Toroid.in :hold @18:24:55.47 Toroid.seek :bypass @18:24:57.18 Toroid.in :default @18:24:57.30 Toroid.seek :clear @18:24:59.27 Carousel.seek 48 @18:24:59.74 Toroid.in :hold @18:24:59.88 Toroid.seek :cartridge @18:25:01.75 Toroid.in :default @18:25:01.89 Toroid.seek :clear @18:25:03.75 delay 2 seconds @18:25:05.88 Toroid.in :hold @18:25:06.00 Toroid.seek :bypass @18:25:07.70 Toroid.in :default @18:25:07.83 Toroid.seek :clear @18:25:09.83 Carousel.seek 49 @18:25:10.29 Toroid.in :hold @18:25:10.43 Toroid.seek :cartridge @18:25:12.31 Toroid.in :default @18:25:12.43 Toroid.seek :clear @18:25:14.29 delay 2 seconds @18:25:16.41 Toroid.in :hold @18:25:16.55 Toroid.seek :bypass @18:25:18.99 Toroid.in :default @18:25:19.14 Toroid.seek :clear @18:25:21.11 Carousel.seek 50 @18:25:21.57 Toroid.in :hold @18:25:21.74 Toroid.seek :cartridge @18:25:23.63 Toroid.in :default @18:25:23.78 Toroid.seek :clear @18:25:25.66 delay 2 seconds @18:25:27.78 Toroid.in :hold @18:25:27.91 Toroid.seek :bypass @18:25:29.60 Toroid.in :default @18:25:29.76 Toroid.seek :clear @18:25:31.77 Carousel.seek 51 @18:25:32.23 Toroid.in :hold @18:25:32.36 Toroid.seek :cartridge @18:25:34.25 Toroid.in :default @18:25:34.38 Toroid.seek :clear @18:25:36.25 delay 2 seconds @18:25:38.37 Toroid.in :hold @18:25:38.50 Toroid.seek :bypass @18:25:40.19 Toroid.in :default @18:25:40.32 Toroid.seek :clear @18:25:42.29 Carousel.seek 52 @18:25:42.76 Toroid.in :hold @18:25:42.89 Toroid.seek :cartridge @18:25:44.78 Toroid.in :default @18:25:44.91 Toroid.seek :clear @18:25:46.76 delay 2 seconds @18:25:48.88 Toroid.in :hold @18:25:49.01 Toroid.seek :bypass @18:25:50.71 Toroid.in :default @18:25:50.84 Toroid.seek :clear @18:25:52.80 Carousel.seek 53 @18:25:53.28 Toroid.in :hold @18:25:53.40 Toroid.seek :cartridge @18:25:55.29 Toroid.in :default @18:25:55.42 Toroid.seek :clear @18:25:57.28 delay 2 seconds @18:25:59.40 Toroid.in :hold @18:25:59.53 Toroid.seek :bypass @18:26:02.00 Toroid.in :default @18:26:02.13 Toroid.seek :clear @18:26:04.12 Carousel.seek 54 @18:26:04.59 Toroid.in :hold @18:26:04.72 Toroid.seek :cartridge @18:26:06.61 Toroid.in :default @18:26:06.74 Toroid.seek :clear @18:26:08.60 delay 2 seconds @18:26:10.72 Toroid.in :hold @18:26:10.85 Toroid.seek :bypass @18:26:12.53 Toroid.in :default @18:26:12.66 Toroid.seek :clear @18:26:14.62 Carousel.seek 55 @18:26:15.09 Toroid.in :hold @18:26:15.22 Toroid.seek :cartridge @18:26:17.11 Toroid.in :default @18:26:17.24 Toroid.seek :clear @18:26:19.10 delay 2 seconds @18:26:21.23 Toroid.in :hold @18:26:21.36 Toroid.seek :bypass @18:26:23.05 Toroid.in :default @18:26:23.17 Toroid.seek :clear @18:26:25.13 Carousel.seek 56 @18:26:25.59 Toroid.in :hold @18:26:25.77 Toroid.seek :cartridge @18:26:27.69 Toroid.in :default @18:26:27.82 Toroid.seek :clear @18:26:29.68 delay 2 seconds @18:26:31.93 Toroid.in :hold @18:26:32.06 Toroid.seek :bypass @18:26:33.86 Toroid.in :default @18:26:33.99 Toroid.seek :clear @18:26:35.98 Carousel.seek 57 @18:26:36.42 Toroid.in :hold @18:26:36.55 Toroid.seek :cartridge @18:26:38.44 Toroid.in :default @18:26:38.57 Toroid.seek :clear @18:26:40.42 delay 2 seconds @18:26:42.54 Toroid.in :hold @18:26:42.68 Toroid.seek :bypass @18:26:45.12 Toroid.in :default @18:26:45.25 Toroid.seek :clear @18:26:47.22 Carousel.seek 58 @18:26:47.69 Toroid.in :hold @18:26:47.83 Toroid.seek :cartridge @18:26:49.71 Toroid.in :default @18:26:49.84 Toroid.seek :clear @18:26:51.69 delay 2 seconds @18:26:53.81 Toroid.in :hold @18:26:53.99 Toroid.seek :bypass @18:26:55.75 Toroid.in :default @18:26:55.88 Toroid.seek :clear @18:26:57.89 Carousel.seek 59 @18:26:58.36 Toroid.in :hold @18:26:58.49 Toroid.seek :cartridge @18:27:00.38 Toroid.in :default @18:27:00.51 Toroid.seek :clear @18:27:02.37 delay 2 seconds @18:27:04.49 Toroid.in :hold @18:27:04.62 Toroid.seek :bypass @18:27:06.32 Toroid.in :default @18:27:06.45 Toroid.seek :clear @18:27:08.41 Carousel.seek 60 @18:27:08.88 Toroid.in :hold @18:27:09.01 Toroid.seek :cartridge @18:27:10.89 Toroid.in :default @18:27:11.02 Toroid.seek :clear @18:27:12.88 delay 2 seconds @18:27:15.00 Toroid.in :hold @18:27:15.14 Toroid.seek :bypass @18:27:16.82 Toroid.in :default @18:27:16.95 Toroid.seek :clear @18:27:18.63 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @18:29:19.96 Session Begun @18:29:26.23 -> SC.to 10 @18:29:26.30 Carousel.seek 10 Storage Carousel (SC) at 10 @18:29:44.35 -> SC.to 50 @18:29:44.45 Carousel.seek 50 Storage Carousel (SC) at 50 @18:30:26.18 -> SC.to 55 @18:30:26.25 Carousel.seek 55 Storage Carousel (SC) at 55 @18:36:27.07 -> SC.to 44 @18:36:27.11 Carousel.seek 44 Storage Carousel (SC) at 44 @18:42:09.69 -> SC.to 31 @18:42:10.58 Carousel.seek 31 Storage Carousel (SC) at 31 @18:46:19.10 -> SC.to 18 @18:46:19.18 Carousel.seek 18 Storage Carousel (SC) at 18 @18:51:06.68 -> SC.to 5 @18:51:06.76 Carousel.seek 5 Storage Carousel (SC) at 5 @18:51:44.78 -> SC.to 10 @18:51:44.88 Carousel.seek 10 Storage Carousel (SC) at 10 @18:54:56.58 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @18:54:57.49 Toroid.seek :clear @18:54:57.70 Carousel.in :default @18:54:57.92 Carousel.home.seek 10 @18:54:58.29 Waiting for bill to answer "Turn the pump on. Push Return" @18:55:04.25 Answered Turn the pump on. Push Return"" @18:55:04.28 Vacuuming individual cartridges. @18:55:05.24 Toroid.seek :clear @18:55:05.40 Carousel.in :default @18:55:05.59 Carousel.home.seek 10 @18:55:06.29 Carousel.seek 1 @18:55:08.55 Lysis1.in :fast @18:55:08.70 Lysis1.seek :locked @18:55:11.39 Plunge.in :default @18:55:11.58 Plunge.seek :locked @18:55:11.83 Air.in :fast @18:55:11.98 Air.seek :locked @18:55:13.66 Carousel.reconfigure :locked @18:55:13.93 Powering cartridge bus @18:55:16.62 Gate.setIR 1750,5000,0,6 @18:55:16.76 Gate.setIR 0,5000,0,6 @18:55:16.89 PuckHeater.configure :default #ambient 23.8C @18:55:17.05 Gate.power :heat,:ON,limit to 5000mA @18:55:17.82 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @18:55:17.93 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:55:18.03 Vacuuming Vent Top cartridge 1 for 30 seconds @18:55:18.07 Air.in :default @18:55:18.26 Air.seek :evac @18:55:32.16 Toroid.in :hold @18:55:32.31 Toroid.seek :cartridge @18:55:34.21 Toroid.in :default @18:55:34.38 Toroid.seek :clear @18:55:36.25 Polling Can every 2:00 @18:55:36.81 PuckHeater.configure :default #ambient 24.1C @18:55:36.95 PuckHeater.seek 41C,1:00 @18:55:58.62 PuckHeater.hold 3 seconds,41C @18:56:01.79 delayUntil "18:56:05.33" @18:56:05.44 Toroid.in :hold @18:56:05.59 Toroid.seek :bypass @18:56:07.34 Toroid.in :default @18:56:07.48 Toroid.seek :clear @18:56:09.18 delay 2 seconds @18:56:11.21 Air.seek :full @18:56:25.90 Gate.power :heat,:OFF @18:56:25.93 Gate.power :cartridge,:OFF @18:56:26.14 Carousel.in :default @18:56:26.35 Carousel.home.seek 1 @18:56:26.87 Air.in :unlocking @18:56:27.11 Air.seek :clear @18:56:28.25 Lysis1.in :unlocking @18:56:28.51 Lysis1.seek :clear @18:56:28.62 Plunge.in :unlocking @18:56:28.84 Plunge.seek :clear @18:56:30.04 Carousel.seek 2 @18:56:30.68 Lysis1.in :fast @18:56:30.83 Lysis1.seek :locked @18:56:33.55 Plunge.in :default @18:56:33.77 Plunge.seek :locked @18:56:34.77 Air.in :fast @18:56:34.90 Air.seek :locked @18:56:36.54 Carousel.reconfigure :locked @18:56:36.80 Powering cartridge bus @18:56:39.49 Gate.setIR 1750,5000,0,6 @18:56:39.64 Gate.setIR 0,5000,0,6 @18:56:39.79 PuckHeater.configure :default #ambient 24.1C @18:56:39.95 Gate.power :heat,:ON,limit to 5000mA @18:56:40.69 Mounted lysis Cartridge board#Q05087 @18:56:40.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:56:40.89 Vacuuming Vent Top cartridge 2 for 30 seconds @18:56:40.98 Air.in :default @18:56:41.13 Air.seek :evac @18:56:54.28 Toroid.in :hold @18:56:54.43 Toroid.seek :cartridge @18:56:56.33 Toroid.in :default @18:56:56.48 Toroid.seek :clear @18:56:58.36 Polling Can every 2:00 @18:56:58.92 PuckHeater.configure :default #ambient 24.1C @18:56:59.05 PuckHeater.seek 41C,1:00 @18:57:20.69 PuckHeater.hold 3 seconds,41C @18:57:23.99 delayUntil "18:57:27.45" @18:57:27.56 Toroid.in :hold @18:57:27.76 Toroid.seek :bypass @18:57:29.48 Toroid.in :default @18:57:29.66 Toroid.seek :clear @18:57:31.36 delay 2 seconds @18:57:33.39 Air.seek :full @18:57:48.09 Gate.power :heat,:OFF @18:57:48.12 Gate.power :cartridge,:OFF @18:57:48.33 Carousel.in :default @18:57:48.55 Carousel.home.seek 2 @18:57:49.09 Air.in :unlocking @18:57:49.29 Air.seek :clear @18:57:51.15 Lysis1.in :unlocking @18:57:51.38 Lysis1.seek :clear @18:57:51.58 Plunge.in :unlocking @18:57:51.78 Plunge.seek :clear @18:57:52.96 Carousel.seek 3 @18:57:53.61 Lysis1.in :fast @18:57:53.78 Lysis1.seek :locked @18:57:56.49 Plunge.in :default @18:57:56.69 Plunge.seek :locked @18:57:56.90 Air.in :fast @18:57:57.04 Air.seek :locked @18:57:58.69 Carousel.reconfigure :locked @18:57:58.94 Powering cartridge bus @18:58:01.64 Gate.setIR 1750,5000,0,6 @18:58:01.78 Gate.setIR 0,5000,0,6 @18:58:01.93 Gate.power :cartridge,:OFF @18:58:02.56 Mounted archive Cartridge @18:58:02.69 Vacuuming Solid Top cartridge 3 for 30 seconds @18:58:02.88 Toroid.in :hold @18:58:03.02 Toroid.seek :cartridge @18:58:04.93 Toroid.in :default @18:58:05.08 Toroid.seek :clear @18:58:06.95 delay 30 seconds @18:58:37.07 Toroid.in :hold @18:58:37.23 Toroid.seek :bypass @18:58:38.94 Toroid.in :default @18:58:39.08 Toroid.seek :clear @18:58:41.01 Carousel.in :default @18:58:41.23 Carousel.home.seek 3 @18:58:41.80 Air.in :unlocking @18:58:42.02 Air.seek :clear @18:58:43.47 Lysis1.in :unlocking @18:58:43.76 Lysis1.seek :clear @18:58:43.82 Plunge.in :unlocking @18:58:44.09 Plunge.seek :clear @18:58:45.30 Carousel.seek 4 @18:58:45.93 Lysis1.in :fast @18:58:46.09 Lysis1.seek :locked @18:58:49.54 Plunge.in :default @18:58:49.76 Plunge.seek :locked @18:58:50.00 Air.in :fast @18:58:50.15 Air.seek :locked @18:58:51.80 Carousel.reconfigure :locked @18:58:52.08 Powering cartridge bus @18:58:54.76 Gate.setIR 1750,5000,0,6 @18:58:54.90 Gate.setIR 0,5000,0,6 @18:58:55.05 PuckHeater.configure :default #ambient 24.1C @18:58:55.20 Gate.power :heat,:ON,limit to 5000mA @18:58:55.91 Mounted lysis Cartridge board#Q05051 @18:58:56.03 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:58:56.10 Vacuuming Vent Top cartridge 4 for 30 seconds @18:58:56.16 Air.in :default @18:58:56.35 Air.seek :evac @18:59:09.48 Toroid.in :hold @18:59:09.67 Toroid.seek :cartridge @18:59:11.56 Toroid.in :default @18:59:11.75 Toroid.seek :clear @18:59:13.67 Polling Can every 2:00 @18:59:14.26 PuckHeater.configure :default #ambient 24.4C @18:59:14.40 PuckHeater.seek 40.9C,1:00 @18:59:36.09 PuckHeater.hold 3 seconds,40.9C @18:59:39.31 delayUntil "18:59:42.69" @18:59:42.79 Toroid.in :hold @18:59:42.95 Toroid.seek :bypass @18:59:44.65 Toroid.in :default @18:59:44.80 Toroid.seek :clear @18:59:46.48 delay 2 seconds @18:59:48.51 Air.seek :full @19:00:03.21 Gate.power :heat,:OFF @19:00:03.24 Gate.power :cartridge,:OFF @19:00:03.45 Carousel.in :default @19:00:03.70 Carousel.home.seek 4 @19:00:04.22 Air.in :unlocking @19:00:04.41 Air.seek :clear @19:00:06.27 Lysis1.in :unlocking @19:00:06.52 Lysis1.seek :clear @19:00:06.59 Plunge.in :unlocking @19:00:06.85 Plunge.seek :clear @19:00:08.05 Carousel.seek 5 @19:00:08.70 Lysis1.in :fast @19:00:08.85 Lysis1.seek :locked @19:00:11.56 Plunge.in :default @19:00:11.78 Plunge.seek :locked @19:00:11.99 Air.in :fast @19:00:12.14 Air.seek :locked @19:00:13.78 Carousel.reconfigure :locked @19:00:14.05 Powering cartridge bus @19:00:16.74 Gate.setIR 1750,5000,0,6 @19:00:16.88 Gate.setIR 0,5000,0,6 @19:00:17.02 Gate.power :cartridge,:OFF @19:00:17.66 Mounted archive Cartridge @19:00:17.78 Vacuuming Solid Top cartridge 5 for 30 seconds @19:00:17.96 Toroid.in :hold @19:00:18.11 Toroid.seek :cartridge @19:00:20.02 Toroid.in :default @19:00:20.16 Toroid.seek :clear @19:00:22.04 delay 30 seconds @19:00:52.16 Toroid.in :hold @19:00:52.32 Toroid.seek :bypass @19:00:54.03 Toroid.in :default @19:00:54.17 Toroid.seek :clear @19:00:56.10 Carousel.in :default @19:00:56.33 Carousel.home.seek 5 @19:00:56.87 Air.in :unlocking @19:00:57.08 Air.seek :clear @19:00:58.52 Lysis1.in :unlocking @19:00:58.76 Lysis1.seek :clear @19:00:58.96 Plunge.in :unlocking @19:00:59.13 Plunge.seek :clear @19:01:00.31 Carousel.seek 6 @19:01:00.94 Lysis1.in :fast @19:01:01.87 Lysis1.seek :locked @19:01:04.59 Plunge.in :default @19:01:04.75 Plunge.seek :locked @19:01:05.01 Air.in :fast @19:01:05.16 Air.seek :locked @19:01:06.81 Carousel.reconfigure :locked @19:01:07.06 Powering cartridge bus @19:01:09.77 Gate.setIR 1750,5000,0,6 @19:01:09.97 Gate.setIR 0,5000,0,6 @19:01:10.13 PuckHeater.configure :default #ambient 24.4C @19:01:10.26 Gate.power :heat,:ON,limit to 5000mA @19:01:10.99 Mounted lysis Cartridge board#Q05049 @19:01:11.10 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:01:11.15 Vacuuming Vent Top cartridge 6 for 30 seconds @19:01:11.23 Air.in :default @19:01:11.41 Air.seek :evac @19:01:24.55 Toroid.in :hold @19:01:24.70 Toroid.seek :cartridge @19:01:26.60 Toroid.in :default @19:01:26.75 Toroid.seek :clear @19:01:28.63 Polling Can every 2:00 @19:01:29.22 PuckHeater.configure :default #ambient 24.4C @19:01:29.35 PuckHeater.seek 40.9C,1:00 @19:01:51.03 PuckHeater.hold 3 seconds,40.9C @19:01:54.28 delayUntil "19:01:57.72" @19:01:57.84 Toroid.in :hold @19:01:58.00 Toroid.seek :bypass @19:01:59.71 Toroid.in :default @19:01:59.86 Toroid.seek :clear @19:02:01.55 delay 2 seconds @19:02:03.58 Air.seek :full @19:02:18.34 Gate.power :heat,:OFF @19:02:18.37 Gate.power :cartridge,:OFF @19:02:18.59 Carousel.in :default @19:02:18.80 Carousel.home.seek 6 @19:02:20.09 Air.in :unlocking @19:02:20.30 Air.seek :clear @19:02:21.38 Lysis1.in :unlocking @19:02:21.60 Lysis1.seek :clear @19:02:21.82 Plunge.in :unlocking @19:02:21.97 Plunge.seek :clear @19:02:23.14 Carousel.seek 7 @19:02:23.82 Lysis1.in :fast @19:02:23.96 Lysis1.seek :locked @19:02:26.67 Plunge.in :default @19:02:26.83 Plunge.seek :locked @19:02:27.08 Air.in :fast @19:02:27.23 Air.seek :locked @19:02:28.87 Carousel.reconfigure :locked @19:02:29.13 Powering cartridge bus @19:02:31.79 Gate.setIR 1750,5000,0,6 @19:02:31.93 Gate.setIR 0,5000,0,6 @19:02:32.07 Gate.power :cartridge,:OFF @19:02:32.71 Mounted archive Cartridge @19:02:32.84 Vacuuming Solid Top cartridge 7 for 30 seconds @19:02:33.02 Toroid.in :hold @19:02:33.18 Toroid.seek :cartridge @19:02:35.09 Toroid.in :default @19:02:35.24 Toroid.seek :clear @19:02:37.11 delay 30 seconds @19:03:07.24 Toroid.in :hold @19:03:07.39 Toroid.seek :bypass @19:03:09.10 Toroid.in :default @19:03:09.26 Toroid.seek :clear @19:03:11.17 Carousel.in :default @19:03:11.40 Carousel.home.seek 7 @19:03:11.93 Air.in :unlocking @19:03:12.16 Air.seek :clear @19:03:13.69 Lysis1.in :unlocking @19:03:13.95 Lysis1.seek :clear @19:03:14.02 Plunge.in :unlocking @19:03:14.29 Plunge.seek :clear @19:03:15.53 Carousel.seek 8 @19:03:16.97 Lysis1.in :fast @19:03:17.12 Lysis1.seek :locked @19:03:19.83 Plunge.in :default @19:03:20.01 Plunge.seek :locked @19:03:20.22 Air.in :fast @19:03:20.37 Air.seek :locked @19:03:22.02 Carousel.reconfigure :locked @19:03:22.29 Powering cartridge bus @19:03:24.96 Gate.setIR 1750,5000,0,6 @19:03:25.10 Gate.setIR 0,5000,0,6 @19:03:25.23 PuckHeater.configure :default #ambient 24.4C @19:03:25.38 Gate.power :heat,:ON,limit to 5000mA @19:03:26.10 Mounted lysis Cartridge board#Q05061 @19:03:26.22 Using this cartridge's heat loss curve for HeatingAccelInCarousel @19:03:26.27 Vacuuming Vent Top cartridge 8 for 30 seconds @19:03:26.34 Air.in :default @19:03:26.60 Air.seek :evac @19:03:39.79 Toroid.in :hold @19:03:39.94 Toroid.seek :cartridge @19:03:41.84 Toroid.in :default @19:03:41.98 Toroid.seek :clear @19:03:43.86 Polling Can every 2:00 @19:03:44.42 PuckHeater.configure :default #ambient 24.5C @19:03:44.56 PuckHeater.seek 41.1C,1:00 @19:04:06.23 PuckHeater.hold 3 seconds,41.1C @19:04:09.42 delayUntil "19:04:12.96" @19:04:13.07 Toroid.in :hold @19:04:13.22 Toroid.seek :bypass @19:04:14.94 Toroid.in :default @19:04:15.09 Toroid.seek :clear @19:04:16.78 delay 2 seconds @19:04:18.82 Air.seek :full @19:04:33.52 Gate.power :heat,:OFF @19:04:33.55 Gate.power :cartridge,:OFF @19:04:33.79 Carousel.in :default @19:04:34.76 Carousel.home.seek 8 @19:04:35.29 Air.in :unlocking @19:04:35.54 Air.seek :clear @19:04:36.74 Lysis1.in :unlocking @19:04:37.02 Lysis1.seek :clear @19:04:37.08 Plunge.in :unlocking @19:04:37.32 Plunge.seek :clear @19:04:38.55 Carousel.seek 9 @19:04:39.18 Lysis1.in :fast @19:04:39.33 Lysis1.seek :locked @19:04:42.03 Plunge.in :default @19:04:42.19 Plunge.seek :locked @19:04:42.44 Air.in :fast @19:04:42.59 Air.seek :locked @19:04:44.24 Carousel.reconfigure :locked @19:04:44.51 Powering cartridge bus @19:04:47.21 Gate.setIR 1750,5000,0,6 @19:04:47.35 Gate.setIR 0,5000,0,6 @19:04:47.49 Gate.power :cartridge,:OFF @19:04:48.12 Mounted archive Cartridge @19:04:48.24 Vacuuming Solid Top cartridge 9 for 30 seconds @19:04:48.43 Toroid.in :hold @19:04:48.58 Toroid.seek :cartridge @19:04:50.50 Toroid.in :default @19:04:50.66 Toroid.seek :clear @19:04:52.53 delay 30 seconds @19:05:22.66 Toroid.in :hold @19:05:22.81 Toroid.seek :bypass @19:05:24.52 Toroid.in :default @19:05:24.67 Toroid.seek :clear @19:05:26.61 Carousel.in :default @19:05:26.94 Carousel.home.seek 9 @19:05:27.48 Air.in :unlocking @19:05:27.76 Air.seek :clear @19:05:29.28 Lysis1.in :unlocking @19:05:29.53 Lysis1.seek :clear @19:05:29.68 Plunge.in :unlocking @19:05:30.68 Plunge.seek :clear @19:05:31.87 Carousel.seek 10 @19:05:32.51 Lysis1.in :fast @19:05:32.66 Lysis1.seek :locked @19:05:35.37 Plunge.in :default @19:05:35.57 Plunge.seek :locked @19:05:35.81 Air.in :fast @19:05:35.95 Air.seek :locked @19:05:37.59 Carousel.reconfigure :locked @19:05:37.90 Powering cartridge bus @19:05:40.57 Gate.setIR 1750,5000,0,6 @19:05:40.71 Gate.setIR 0,5000,0,6 @19:05:40.85 PuckHeater.configure :default #ambient 24.5C @19:05:41.01 Gate.power :heat,:ON,limit to 5000mA @19:05:41.75 Mounted lysis Cartridge board#Q05060 @19:05:41.86 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:05:41.95 Vacuuming Vent Top cartridge 10 for 30 seconds @19:05:41.99 Air.in :default @19:05:42.19 Air.seek :evac @19:05:55.31 Toroid.in :hold @19:05:55.46 Toroid.seek :cartridge @19:05:57.37 Toroid.in :default @19:05:57.52 Toroid.seek :clear @19:05:59.45 Polling Can every 2:00 @19:06:00.01 PuckHeater.configure :default #ambient 24.6C @19:06:00.15 PuckHeater.seek 40.9C,1:00 @19:06:21.82 PuckHeater.hold 3 seconds,40.9C @19:06:25.09 delayUntil "19:06:28.48" @19:06:28.59 Toroid.in :hold @19:06:28.74 Toroid.seek :bypass @19:06:30.45 Toroid.in :default @19:06:30.60 Toroid.seek :clear @19:06:32.28 delay 2 seconds @19:06:34.31 Air.seek :full @19:06:49.67 Gate.power :heat,:OFF @19:06:49.87 Gate.power :cartridge,:OFF @19:06:50.02 Carousel.in :default @19:06:50.24 Carousel.home.seek 10 @19:06:50.77 Air.in :unlocking @19:06:51.00 Air.seek :clear @19:06:52.19 Lysis1.in :unlocking @19:06:52.41 Lysis1.seek :clear @19:06:52.62 Plunge.in :unlocking @19:06:52.77 Plunge.seek :clear @19:06:53.95 Carousel.seek 11 @19:06:54.60 Lysis1.in :fast @19:06:54.75 Lysis1.seek :locked @19:06:57.45 Plunge.in :default @19:06:57.67 Plunge.seek :locked @19:06:57.90 Air.in :fast @19:06:58.05 Air.seek :locked @19:06:59.69 Carousel.reconfigure :locked @19:06:59.95 Powering cartridge bus @19:07:02.62 Gate.setIR 1750,5000,0,6 @19:07:02.76 Gate.setIR 0,5000,0,6 @19:07:02.89 Gate.power :cartridge,:OFF @19:07:03.51 Mounted archive Cartridge @19:07:03.66 Vacuuming Solid Top cartridge 11 for 30 seconds @19:07:03.85 Toroid.in :hold @19:07:04.02 Toroid.seek :cartridge @19:07:05.93 Toroid.in :default @19:07:06.09 Toroid.seek :clear @19:07:07.96 delay 30 seconds @19:07:38.09 Toroid.in :hold @19:07:38.29 Toroid.seek :bypass @19:07:40.00 Toroid.in :default @19:07:40.15 Toroid.seek :clear @19:07:42.08 Carousel.in :default @19:07:42.30 Carousel.home.seek 11 @19:07:42.83 Air.in :unlocking @19:07:43.04 Air.seek :clear @19:07:44.48 Lysis1.in :unlocking @19:07:45.51 Lysis1.seek :clear @19:07:45.58 Plunge.in :unlocking @19:07:45.89 Plunge.seek :clear @19:07:47.08 Carousel.seek 12 @19:07:47.75 Lysis1.in :fast @19:07:47.90 Lysis1.seek :locked @19:07:50.62 Plunge.in :default @19:07:50.81 Plunge.seek :locked @19:07:51.01 Air.in :fast @19:07:51.15 Air.seek :locked @19:07:52.80 Carousel.reconfigure :locked @19:07:53.05 Powering cartridge bus @19:07:55.77 Gate.setIR 1750,5000,0,6 @19:07:55.90 Gate.setIR 0,5000,0,6 @19:07:56.03 PuckHeater.configure :default #ambient 24.6C @19:07:56.18 Gate.power :heat,:ON,limit to 5000mA @19:07:56.88 Mounted lysis Cartridge board#Q05062 @19:07:56.99 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:07:57.06 Vacuuming Vent Top cartridge 12 for 30 seconds @19:07:57.11 Air.in :default @19:07:57.29 Air.seek :evac @19:08:10.42 Toroid.in :hold @19:08:10.57 Toroid.seek :cartridge @19:08:12.47 Toroid.in :default @19:08:12.62 Toroid.seek :clear @19:08:14.50 Polling Can every 2:00 @19:08:15.06 PuckHeater.configure :default #ambient 24.6C @19:08:15.19 PuckHeater.seek 40.9C,1:00 @19:08:36.88 PuckHeater.hold 3 seconds,40.9C @19:08:40.09 delayUntil "19:08:43.59" @19:08:43.73 Toroid.in :hold @19:08:43.88 Toroid.seek :bypass @19:08:45.58 Toroid.in :default @19:08:45.78 Toroid.seek :clear @19:08:47.48 delay 2 seconds @19:08:50.17 Air.seek :full @19:09:04.97 Gate.power :heat,:OFF @19:09:05.00 Gate.power :cartridge,:OFF @19:09:05.22 Carousel.in :default @19:09:05.43 Carousel.home.seek 12 @19:09:05.98 Air.in :unlocking @19:09:06.20 Air.seek :clear @19:09:07.29 Lysis1.in :unlocking @19:09:07.48 Lysis1.seek :clear @19:09:07.74 Plunge.in :unlocking @19:09:07.90 Plunge.seek :clear @19:09:09.06 Carousel.seek 13 @19:09:09.73 Lysis1.in :fast @19:09:09.89 Lysis1.seek :locked @19:09:12.61 Plunge.in :default @19:09:12.79 Plunge.seek :locked @19:09:13.01 Air.in :fast @19:09:13.14 Air.seek :locked @19:09:14.80 Carousel.reconfigure :locked @19:09:15.07 Powering cartridge bus @19:09:17.77 Gate.setIR 1750,5000,0,6 @19:09:17.90 Gate.setIR 0,5000,0,6 @19:09:18.05 Gate.power :cartridge,:OFF @19:09:18.68 Mounted archive Cartridge @19:09:18.81 Vacuuming Solid Top cartridge 13 for 30 seconds @19:09:18.99 Toroid.in :hold @19:09:19.15 Toroid.seek :cartridge @19:09:21.05 Toroid.in :default @19:09:21.20 Toroid.seek :clear @19:09:23.09 delay 30 seconds @19:09:53.22 Toroid.in :hold @19:09:53.42 Toroid.seek :bypass @19:09:55.12 Toroid.in :default @19:09:55.28 Toroid.seek :clear @19:09:57.21 Carousel.in :default @19:09:57.42 Carousel.home.seek 13 @19:09:57.96 Air.in :unlocking @19:09:58.16 Air.seek :clear @19:09:59.66 Lysis1.in :unlocking @19:09:59.86 Lysis1.seek :clear @19:10:00.05 Plunge.in :unlocking @19:10:00.21 Plunge.seek :clear @19:10:01.40 Carousel.seek 14 @19:10:02.02 Lysis1.in :fast @19:10:02.17 Lysis1.seek :locked @19:10:04.87 Plunge.in :default @19:10:05.06 Plunge.seek :locked @19:10:05.27 Air.in :fast @19:10:05.42 Air.seek :locked @19:10:07.08 Carousel.reconfigure :locked @19:10:07.36 Powering cartridge bus @19:10:10.04 Gate.setIR 1750,5000,0,6 @19:10:10.18 Gate.setIR 0,5000,0,6 @19:10:10.32 PuckHeater.configure :default #ambient 24.6C @19:10:10.48 Gate.power :heat,:ON,limit to 5000mA @19:10:11.19 Mounted lysis Cartridge board#Q05036 @19:10:11.30 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:10:11.34 Vacuuming Vent Top cartridge 14 for 30 seconds @19:10:11.42 Air.in :default @19:10:11.61 Air.seek :evac @19:10:24.77 Toroid.in :hold @19:10:24.92 Toroid.seek :cartridge @19:10:26.84 Toroid.in :default @19:10:27.00 Toroid.seek :clear @19:10:28.86 Polling Can every 2:00 @19:10:29.42 PuckHeater.configure :default #ambient 24.6C @19:10:29.56 PuckHeater.seek 40.9C,1:00 @19:10:51.21 PuckHeater.hold 3 seconds,40.9C @19:10:54.48 delayUntil "19:10:57.94" @19:10:58.05 Toroid.in :hold @19:10:58.20 Toroid.seek :bypass @19:11:00.56 Toroid.in :default @19:11:00.82 Toroid.seek :clear @19:11:02.51 delay 2 seconds @19:11:04.54 Air.seek :full @19:11:19.24 Gate.power :heat,:OFF @19:11:19.27 Gate.power :cartridge,:OFF @19:11:19.48 Carousel.in :default @19:11:19.70 Carousel.home.seek 14 @19:11:20.23 Air.in :unlocking @19:11:20.46 Air.seek :clear @19:11:21.64 Lysis1.in :unlocking @19:11:21.89 Lysis1.seek :clear @19:11:22.09 Plunge.in :unlocking @19:11:22.26 Plunge.seek :clear @19:11:23.43 Carousel.seek 15 @19:11:24.06 Lysis1.in :fast @19:11:24.22 Lysis1.seek :locked @19:11:26.91 Plunge.in :default @19:11:27.06 Plunge.seek :locked @19:11:27.31 Air.in :fast @19:11:27.46 Air.seek :locked @19:11:29.11 Carousel.reconfigure :locked @19:11:29.38 Powering cartridge bus @19:11:32.05 Gate.setIR 1750,5000,0,6 @19:11:32.19 Gate.setIR 0,5000,0,6 @19:11:32.33 Gate.power :cartridge,:OFF @19:11:32.96 Mounted archive Cartridge @19:11:33.08 Vacuuming Solid Top cartridge 15 for 30 seconds @19:11:33.27 Toroid.in :hold @19:11:33.41 Toroid.seek :cartridge @19:11:35.33 Toroid.in :default @19:11:35.48 Toroid.seek :clear @19:11:37.36 delay 30 seconds @19:12:07.48 Toroid.in :hold @19:12:07.71 Toroid.seek :bypass @19:12:09.48 Toroid.in :default @19:12:09.68 Toroid.seek :clear @19:12:11.65 Carousel.in :default @19:12:11.87 Carousel.home.seek 15 @19:12:13.18 Air.in :unlocking @19:12:13.44 Air.seek :clear @19:12:15.00 Lysis1.in :unlocking @19:12:15.27 Lysis1.seek :clear @19:12:15.37 Plunge.in :unlocking @19:12:15.60 Plunge.seek :clear @19:12:16.84 Carousel.seek 16 @19:12:17.48 Lysis1.in :fast @19:12:17.66 Lysis1.seek :locked @19:12:20.37 Plunge.in :default @19:12:20.54 Plunge.seek :locked @19:12:20.77 Air.in :fast @19:12:20.93 Air.seek :locked @19:12:22.56 Carousel.reconfigure :locked @19:12:22.82 Powering cartridge bus @19:12:25.49 Gate.setIR 1750,5000,0,6 @19:12:25.64 Gate.setIR 0,5000,0,6 @19:12:25.80 PuckHeater.configure :default #ambient 24.6C @19:12:25.94 Gate.power :heat,:ON,limit to 5000mA @19:12:26.67 Mounted lysis Cartridge board#Q05080 @19:12:26.78 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:12:26.85 Vacuuming Vent Top cartridge 16 for 30 seconds @19:12:26.95 Air.in :default @19:12:27.09 Air.seek :evac @19:12:40.24 Toroid.in :hold @19:12:40.39 Toroid.seek :cartridge @19:12:42.32 Toroid.in :default @19:12:42.47 Toroid.seek :clear @19:12:44.35 Polling Can every 2:00 @19:12:44.92 PuckHeater.seek 40.9C,1:00 @19:13:06.55 PuckHeater.hold 3 seconds,40.9C @19:13:09.79 delayUntil "19:13:13.41" @19:13:13.52 Toroid.in :hold @19:13:14.45 Toroid.seek :bypass @19:13:16.18 Toroid.in :default @19:13:16.34 Toroid.seek :clear @19:13:18.03 delay 2 seconds @19:13:20.06 Air.seek :full @19:13:34.75 Gate.power :heat,:OFF @19:13:34.78 Gate.power :cartridge,:OFF @19:13:34.99 Carousel.in :default @19:13:35.20 Carousel.home.seek 16 @19:13:35.77 Air.in :unlocking @19:13:36.00 Air.seek :clear @19:13:37.18 Lysis1.in :unlocking @19:13:37.43 Lysis1.seek :clear @19:13:37.50 Plunge.in :unlocking @19:13:37.79 Plunge.seek :clear @19:13:39.01 Carousel.seek 17 @19:13:39.67 Lysis1.in :fast @19:13:39.81 Lysis1.seek :locked @19:13:42.53 Plunge.in :default @19:13:42.68 Plunge.seek :locked @19:13:42.93 Air.in :fast @19:13:43.08 Air.seek :locked @19:13:44.72 Carousel.reconfigure :locked @19:13:44.99 Powering cartridge bus @19:13:47.69 Gate.setIR 1750,5000,0,6 @19:13:47.82 Gate.setIR 0,5000,0,6 @19:13:47.98 Gate.power :cartridge,:OFF @19:13:48.60 Mounted archive Cartridge @19:13:48.73 Vacuuming Solid Top cartridge 17 for 30 seconds @19:13:48.91 Toroid.in :hold @19:13:49.07 Toroid.seek :cartridge @19:13:50.98 Toroid.in :default @19:13:51.13 Toroid.seek :clear @19:13:53.00 delay 30 seconds @19:14:23.14 Toroid.in :hold @19:14:23.34 Toroid.seek :bypass @19:14:25.04 Toroid.in :default @19:14:25.19 Toroid.seek :clear @19:14:27.12 Carousel.in :default @19:14:27.34 Carousel.home.seek 17 @19:14:28.67 Air.in :unlocking @19:14:28.90 Air.seek :clear @19:14:30.43 Lysis1.in :unlocking @19:14:30.69 Lysis1.seek :clear @19:14:30.86 Plunge.in :unlocking @19:14:31.04 Plunge.seek :clear @19:14:32.23 Carousel.seek 18 @19:14:32.86 Lysis1.in :fast @19:14:33.00 Lysis1.seek :locked @19:14:35.82 Plunge.in :default @19:14:36.00 Plunge.seek :locked @19:14:36.06 Air.in :fast @19:14:36.29 Air.seek :locked @19:14:37.95 Carousel.reconfigure :locked @19:14:38.21 Powering cartridge bus @19:14:40.89 Gate.setIR 1750,5000,0,6 @19:14:41.07 Gate.setIR 0,5000,0,6 @19:14:41.22 PuckHeater.configure :default #ambient 24.6C @19:14:41.36 Gate.power :heat,:ON,limit to 5000mA @19:14:42.12 Mounted lysis Cartridge board#Q05092 @19:14:42.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:14:42.32 Vacuuming Vent Top cartridge 18 for 30 seconds @19:14:42.36 Air.in :default @19:14:42.58 Air.seek :evac @19:14:55.72 Toroid.in :hold @19:14:55.87 Toroid.seek :cartridge @19:14:57.77 Toroid.in :default @19:14:57.93 Toroid.seek :clear @19:14:59.81 Polling Can every 2:00 @19:15:00.37 PuckHeater.configure :default #ambient 24.6C @19:15:00.51 PuckHeater.seek 40.9C,1:00 @19:15:22.14 PuckHeater.hold 3 seconds,40.9C @19:15:26.11 delayUntil "19:15:28.89" @19:15:29.00 Toroid.in :hold @19:15:29.15 Toroid.seek :bypass @19:15:30.86 Toroid.in :default @19:15:31.02 Toroid.seek :clear @19:15:32.72 delay 2 seconds @19:15:34.76 Air.seek :full @19:15:49.45 Gate.power :heat,:OFF @19:15:49.52 Gate.power :cartridge,:OFF @19:15:49.72 Carousel.in :default @19:15:49.93 Carousel.home.seek 18 @19:15:50.45 Air.in :unlocking @19:15:50.64 Air.seek :clear @19:15:51.78 Lysis1.in :unlocking @19:15:52.03 Lysis1.seek :clear @19:15:52.10 Plunge.in :unlocking @19:15:52.35 Plunge.seek :clear @19:15:53.56 Carousel.seek 19 @19:15:54.27 Lysis1.in :fast @19:15:54.47 Lysis1.seek :locked @19:15:57.17 Plunge.in :default @19:15:57.36 Plunge.seek :locked @19:15:57.57 Air.in :fast @19:15:57.76 Air.seek :locked @19:15:59.41 Carousel.reconfigure :locked @19:15:59.71 Powering cartridge bus @19:16:02.38 Gate.setIR 1750,5000,0,6 @19:16:02.52 Gate.setIR 0,5000,0,6 @19:16:02.66 Gate.power :cartridge,:OFF @19:16:03.29 Mounted archive Cartridge @19:16:03.41 Vacuuming Solid Top cartridge 19 for 30 seconds @19:16:03.60 Toroid.in :hold @19:16:03.80 Toroid.seek :cartridge @19:16:05.70 Toroid.in :default @19:16:05.85 Toroid.seek :clear @19:16:07.73 delay 30 seconds @19:16:37.86 Toroid.in :hold @19:16:38.06 Toroid.seek :bypass @19:16:39.76 Toroid.in :default @19:16:39.91 Toroid.seek :clear @19:16:41.87 Carousel.in :default @19:16:42.84 Carousel.home.seek 19 @19:16:43.38 Air.in :unlocking @19:16:43.65 Air.seek :clear @19:16:45.15 Lysis1.in :unlocking @19:16:45.40 Lysis1.seek :clear @19:16:45.56 Plunge.in :unlocking @19:16:45.77 Plunge.seek :clear @19:16:46.97 Carousel.seek 20 @19:16:47.59 Lysis1.in :fast @19:16:47.78 Lysis1.seek :locked @19:16:50.48 Plunge.in :default @19:16:50.68 Plunge.seek :locked @19:16:50.90 Air.in :fast @19:16:51.06 Air.seek :locked @19:16:52.68 Carousel.reconfigure :locked @19:16:52.94 Powering cartridge bus @19:16:55.65 Gate.setIR 1750,5000,0,6 @19:16:55.79 Gate.setIR 0,5000,0,6 @19:16:55.94 PuckHeater.configure :default #ambient 24.6C @19:16:56.08 Gate.power :heat,:ON,limit to 5000mA @19:16:56.79 Mounted lysis Cartridge board#Q05065 @19:16:56.89 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:16:56.96 Vacuuming Vent Top cartridge 20 for 30 seconds @19:16:57.05 Air.in :default @19:16:57.21 Air.seek :evac @19:17:10.35 Toroid.in :hold @19:17:10.50 Toroid.seek :cartridge @19:17:12.40 Toroid.in :default @19:17:12.55 Toroid.seek :clear @19:17:14.45 Polling Can every 2:00 @19:17:15.01 PuckHeater.configure :default #ambient 24.6C @19:17:15.14 PuckHeater.seek 40.9C,1:00 @19:17:36.51 PuckHeater.hold 3 seconds,40.9C @19:17:39.73 delayUntil "19:17:43.52" @19:17:43.65 Toroid.in :hold @19:17:43.81 Toroid.seek :bypass @19:17:45.51 Toroid.in :default @19:17:45.72 Toroid.seek :clear @19:17:47.41 delay 2 seconds @19:17:49.44 Air.seek :full @19:18:04.14 Gate.power :heat,:OFF @19:18:04.17 Gate.power :cartridge,:OFF @19:18:04.39 Carousel.in :default @19:18:04.61 Carousel.home.seek 20 @19:18:05.13 Air.in :unlocking @19:18:05.38 Air.seek :clear @19:18:06.55 Lysis1.in :unlocking @19:18:06.77 Lysis1.seek :clear @19:18:06.96 Plunge.in :unlocking @19:18:07.12 Plunge.seek :clear @19:18:08.29 Carousel.seek 21 @19:18:08.91 Lysis1.in :fast @19:18:09.07 Lysis1.seek :locked @19:18:11.78 Plunge.in :default @19:18:11.94 Plunge.seek :locked @19:18:12.19 Air.in :fast @19:18:12.34 Air.seek :locked @19:18:13.98 Carousel.reconfigure :locked @19:18:14.25 Powering cartridge bus @19:18:16.92 Gate.setIR 1750,5000,0,6 @19:18:17.06 Gate.setIR 0,5000,0,6 @19:18:17.22 Gate.power :cartridge,:OFF @19:18:17.83 Mounted archive Cartridge @19:18:17.96 Vacuuming Solid Top cartridge 21 for 30 seconds @19:18:18.15 Toroid.in :hold @19:18:18.30 Toroid.seek :cartridge @19:18:20.20 Toroid.in :default @19:18:20.35 Toroid.seek :clear @19:18:22.23 delay 30 seconds @19:18:52.36 Toroid.in :hold @19:18:52.56 Toroid.seek :bypass @19:18:54.27 Toroid.in :default @19:18:54.42 Toroid.seek :clear @19:18:57.08 Carousel.in :default @19:18:57.31 Carousel.home.seek 21 @19:18:57.91 Air.in :unlocking @19:18:58.20 Air.seek :clear @19:18:59.68 Lysis1.in :unlocking @19:18:59.95 Lysis1.seek :clear @19:19:00.03 Plunge.in :unlocking @19:19:00.28 Plunge.seek :clear @19:19:01.50 Carousel.seek 22 @19:19:02.15 Lysis1.in :fast @19:19:02.30 Lysis1.seek :locked @19:19:05.02 Plunge.in :default @19:19:05.21 Plunge.seek :locked @19:19:05.42 Air.in :fast @19:19:05.57 Air.seek :locked @19:19:07.25 Carousel.reconfigure :locked @19:19:07.50 Powering cartridge bus @19:19:10.18 Gate.setIR 1750,5000,0,6 @19:19:10.32 Gate.setIR 0,5000,0,6 @19:19:10.45 PuckHeater.configure :default #ambient 24.6C @19:19:10.61 Gate.power :heat,:ON,limit to 5000mA @19:19:11.32 Mounted lysis Cartridge board#Q05090 @19:19:11.43 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:19:11.48 Vacuuming Vent Top cartridge 22 for 30 seconds @19:19:11.58 Air.in :default @19:19:11.78 Air.seek :evac @19:19:24.91 Toroid.in :hold @19:19:25.06 Toroid.seek :cartridge @19:19:26.96 Toroid.in :default @19:19:27.12 Toroid.seek :clear @19:19:29.00 Polling Can every 2:00 @19:19:29.56 PuckHeater.configure :default #ambient 24.6C @19:19:29.73 PuckHeater.seek 40.9C,1:00 @19:19:51.48 PuckHeater.hold 3 seconds,40.9C @19:19:54.79 delayUntil "19:19:58.08" @19:19:58.20 Toroid.in :hold @19:19:58.34 Toroid.seek :bypass @19:20:00.07 Toroid.in :default @19:20:00.22 Toroid.seek :clear @19:20:01.87 delay 2 seconds @19:20:03.90 Air.seek :full @19:20:18.61 Gate.power :heat,:OFF @19:20:18.64 Gate.power :cartridge,:OFF @19:20:18.86 Carousel.in :default @19:20:19.06 Carousel.home.seek 22 @19:20:19.60 Air.in :unlocking @19:20:19.88 Air.seek :clear @19:20:21.03 Lysis1.in :unlocking @19:20:21.25 Lysis1.seek :clear @19:20:21.45 Plunge.in :unlocking @19:20:21.65 Plunge.seek :clear @19:20:22.84 Carousel.seek 23 @19:20:23.48 Lysis1.in :fast @19:20:23.65 Lysis1.seek :locked @19:20:26.33 Plunge.in :default @19:20:26.49 Plunge.seek :locked @19:20:26.73 Air.in :fast @19:20:26.87 Air.seek :locked @19:20:28.53 Carousel.reconfigure :locked @19:20:28.79 Powering cartridge bus @19:20:31.48 Gate.setIR 1750,5000,0,6 @19:20:31.63 Gate.setIR 0,5000,0,6 @19:20:31.79 Gate.power :cartridge,:OFF @19:20:32.42 Mounted archive Cartridge @19:20:32.54 Vacuuming Solid Top cartridge 23 for 30 seconds @19:20:32.74 Toroid.in :hold @19:20:32.90 Toroid.seek :cartridge @19:20:34.80 Toroid.in :default @19:20:34.94 Toroid.seek :clear @19:20:36.81 delay 30 seconds @19:21:06.94 Toroid.in :hold @19:21:07.14 Toroid.seek :bypass @19:21:08.85 Toroid.in :default @19:21:09.68 Toroid.seek :clear @19:21:11.76 Carousel.in :default @19:21:11.99 Carousel.home.seek 23 @19:21:12.53 Air.in :unlocking @19:21:12.74 Air.seek :clear @19:21:14.21 Lysis1.in :unlocking @19:21:14.40 Lysis1.seek :clear @19:21:14.64 Plunge.in :unlocking @19:21:14.80 Plunge.seek :clear @19:21:15.98 Carousel.seek 24 @19:21:16.62 Lysis1.in :fast @19:21:16.76 Lysis1.seek :locked @19:21:19.46 Plunge.in :default @19:21:19.69 Plunge.seek :locked @19:21:19.90 Air.in :fast @19:21:20.04 Air.seek :locked @19:21:21.69 Carousel.reconfigure :locked @19:21:21.96 Powering cartridge bus @19:21:24.64 Gate.setIR 1750,5000,0,6 @19:21:24.78 Gate.setIR 0,5000,0,6 @19:21:24.91 PuckHeater.configure :default #ambient 24.6C @19:21:25.06 Gate.power :heat,:ON,limit to 5000mA @19:21:25.79 Mounted lysis Cartridge board#Q05042 @19:21:25.90 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:21:25.99 Vacuuming Vent Top cartridge 24 for 30 seconds @19:21:26.06 Air.in :default @19:21:26.22 Air.seek :evac @19:21:39.36 Toroid.in :hold @19:21:39.52 Toroid.seek :cartridge @19:21:41.45 Toroid.in :default @19:21:41.59 Toroid.seek :clear @19:21:43.52 Polling Can every 2:00 @19:21:44.08 PuckHeater.configure :default #ambient 24.6C @19:21:44.22 PuckHeater.seek 40.9C,1:00 @19:22:05.60 PuckHeater.hold 3 seconds,40.9C @19:22:08.86 delayUntil "19:22:12.54" @19:22:12.65 Toroid.in :hold @19:22:12.81 Toroid.seek :bypass @19:22:14.55 Toroid.in :default @19:22:14.70 Toroid.seek :clear @19:22:16.40 delay 2 seconds @19:22:18.43 Air.seek :full @19:22:33.12 Gate.power :heat,:OFF @19:22:33.15 Gate.power :cartridge,:OFF @19:22:33.36 Carousel.in :default @19:22:33.58 Carousel.home.seek 24 @19:22:34.15 Air.in :unlocking @19:22:34.39 Air.seek :clear @19:22:35.57 Lysis1.in :unlocking @19:22:35.87 Lysis1.seek :clear @19:22:35.95 Plunge.in :unlocking @19:22:36.18 Plunge.seek :clear @19:22:37.39 Carousel.seek 25 @19:22:38.01 Lysis1.in :fast @19:22:38.16 Lysis1.seek :locked @19:22:40.90 Plunge.in :default @19:22:41.08 Plunge.seek :locked @19:22:41.31 Air.in :fast @19:22:41.46 Air.seek :locked @19:22:43.10 Carousel.reconfigure :locked @19:22:43.36 Powering cartridge bus @19:22:46.06 Gate.setIR 1750,5000,0,6 @19:22:46.24 Gate.setIR 0,5000,0,6 @19:22:46.40 Gate.power :cartridge,:OFF @19:22:47.02 Mounted archive Cartridge @19:22:47.14 Vacuuming Solid Top cartridge 25 for 30 seconds @19:22:47.33 Toroid.in :hold @19:22:47.47 Toroid.seek :cartridge @19:22:49.40 Toroid.in :default @19:22:49.55 Toroid.seek :clear @19:22:51.47 delay 30 seconds @19:23:21.59 Toroid.in :hold @19:23:21.83 Toroid.seek :bypass @19:23:24.36 Toroid.in :default @19:23:24.51 Toroid.seek :clear @19:23:26.44 Carousel.in :default @19:23:26.67 Carousel.home.seek 25 @19:23:27.20 Air.in :unlocking @19:23:27.44 Air.seek :clear @19:23:29.01 Lysis1.in :unlocking @19:23:29.30 Lysis1.seek :clear @19:23:29.38 Plunge.in :unlocking @19:23:29.71 Plunge.seek :clear @19:23:30.93 Carousel.seek 26 @19:23:31.57 Lysis1.in :fast @19:23:31.75 Lysis1.seek :locked @19:23:34.45 Plunge.in :default @19:23:34.62 Plunge.seek :locked @19:23:34.85 Air.in :fast @19:23:35.01 Air.seek :locked @19:23:36.67 Carousel.reconfigure :locked @19:23:36.93 Powering cartridge bus @19:23:39.63 Gate.setIR 1750,5000,0,6 @19:23:39.82 Gate.setIR 0,5000,0,6 @19:23:39.98 PuckHeater.configure :default #ambient 24.6C @19:23:40.11 Gate.power :heat,:ON,limit to 5000mA @19:23:40.83 Mounted lysis Cartridge board#Q05059 @19:23:40.93 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:23:40.99 Vacuuming Vent Top cartridge 26 for 30 seconds @19:23:41.12 Air.in :default @19:23:41.26 Air.seek :evac @19:23:54.40 Toroid.in :hold @19:23:54.54 Toroid.seek :cartridge @19:23:56.46 Toroid.in :default @19:23:56.60 Toroid.seek :clear @19:23:58.48 Polling Can every 2:00 @19:23:59.05 PuckHeater.seek 40.9C,1:00 @19:24:20.39 PuckHeater.hold 3 seconds,40.9C @19:24:23.68 delayUntil "19:24:27.56" @19:24:27.71 Toroid.in :hold @19:24:27.86 Toroid.seek :bypass @19:24:29.59 Toroid.in :default @19:24:29.77 Toroid.seek :clear @19:24:31.45 delay 2 seconds @19:24:33.48 Air.seek :full @19:24:48.18 Gate.power :heat,:OFF @19:24:48.21 Gate.power :cartridge,:OFF @19:24:48.43 Carousel.in :default @19:24:48.63 Carousel.home.seek 26 @19:24:49.16 Air.in :unlocking @19:24:49.40 Air.seek :clear @19:24:50.55 Lysis1.in :unlocking @19:24:50.79 Lysis1.seek :clear @19:24:50.86 Plunge.in :unlocking @19:24:51.12 Plunge.seek :clear @19:24:52.34 Carousel.seek 27 @19:24:52.97 Lysis1.in :fast @19:24:53.13 Lysis1.seek :locked @19:24:55.82 Plunge.in :default @19:24:56.01 Plunge.seek :locked @19:24:56.23 Air.in :fast @19:24:56.37 Air.seek :locked @19:24:58.02 Carousel.reconfigure :locked @19:24:58.30 Powering cartridge bus @19:25:00.98 Gate.setIR 1750,5000,0,6 @19:25:01.11 Gate.setIR 0,5000,0,6 @19:25:01.25 Gate.power :cartridge,:OFF @19:25:01.88 Mounted archive Cartridge @19:25:02.01 Vacuuming Solid Top cartridge 27 for 30 seconds @19:25:02.20 Toroid.in :hold @19:25:02.34 Toroid.seek :cartridge @19:25:04.26 Toroid.in :default @19:25:04.40 Toroid.seek :clear @19:25:06.28 delay 30 seconds @19:25:37.03 Toroid.in :hold @19:25:37.33 Toroid.seek :bypass @19:25:39.11 Toroid.in :default @19:25:39.26 Toroid.seek :clear @19:25:41.18 Carousel.in :default @19:25:41.40 Carousel.home.seek 27 @19:25:41.93 Air.in :unlocking @19:25:42.16 Air.seek :clear @19:25:43.72 Lysis1.in :unlocking @19:25:43.99 Lysis1.seek :clear @19:25:44.07 Plunge.in :unlocking @19:25:44.36 Plunge.seek :clear @19:25:45.55 Carousel.seek 28 @19:25:46.14 Lysis1.in :fast @19:25:46.28 Lysis1.seek :locked @19:25:49.01 Plunge.in :default @19:25:49.16 Plunge.seek :locked @19:25:49.47 Air.in :fast @19:25:49.67 Air.seek :locked @19:25:51.34 Carousel.reconfigure :locked @19:25:51.64 Powering cartridge bus @19:25:54.34 Gate.setIR 1750,5000,0,6 @19:25:54.48 Gate.setIR 0,5000,0,6 @19:25:54.62 PuckHeater.configure :default #ambient 24.6C @19:25:54.77 Gate.power :heat,:ON,limit to 5000mA @19:25:55.47 Mounted lysis Cartridge board#Q05086 @19:25:55.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:25:55.67 Vacuuming Vent Top cartridge 28 for 30 seconds @19:25:55.76 Air.in :default @19:25:55.96 Air.seek :evac @19:26:09.08 Toroid.in :hold @19:26:09.24 Toroid.seek :cartridge @19:26:11.14 Toroid.in :default @19:26:11.28 Toroid.seek :clear @19:26:13.17 Polling Can every 2:00 @19:26:13.73 PuckHeater.configure :default #ambient 24.6C @19:26:13.87 PuckHeater.seek 40.9C,1:00 @19:26:35.22 PuckHeater.hold 3 seconds,40.9C @19:26:38.49 delayUntil "19:26:42.25" @19:26:42.37 Toroid.in :hold @19:26:42.52 Toroid.seek :bypass @19:26:44.23 Toroid.in :default @19:26:44.38 Toroid.seek :clear @19:26:46.07 delay 2 seconds @19:26:48.10 Air.seek :full @19:27:02.79 Gate.power :heat,:OFF @19:27:02.82 Gate.power :cartridge,:OFF @19:27:03.03 Carousel.in :default @19:27:03.22 Carousel.home.seek 28 @19:27:03.80 Air.in :unlocking @19:27:04.05 Air.seek :clear @19:27:05.22 Lysis1.in :unlocking @19:27:05.48 Lysis1.seek :clear @19:27:05.55 Plunge.in :unlocking @19:27:05.82 Plunge.seek :clear @19:27:07.03 Carousel.seek 29 @19:27:07.70 Lysis1.in :fast @19:27:07.85 Lysis1.seek :locked @19:27:10.55 Plunge.in :default @19:27:10.71 Plunge.seek :locked @19:27:10.96 Air.in :fast @19:27:11.12 Air.seek :locked @19:27:12.76 Carousel.reconfigure :locked @19:27:13.02 Powering cartridge bus @19:27:15.69 Gate.setIR 1750,5000,0,6 @19:27:15.83 Gate.setIR 0,5000,0,6 @19:27:15.98 Gate.power :cartridge,:OFF @19:27:16.61 Mounted archive Cartridge @19:27:16.73 Vacuuming Solid Top cartridge 29 for 30 seconds @19:27:16.91 Toroid.in :hold @19:27:17.07 Toroid.seek :cartridge @19:27:18.96 Toroid.in :default @19:27:19.11 Toroid.seek :clear @19:27:21.79 delay 30 seconds @19:27:51.92 Toroid.in :hold @19:27:52.13 Toroid.seek :bypass @19:27:53.83 Toroid.in :default @19:27:53.99 Toroid.seek :clear @19:27:55.93 Carousel.in :default @19:27:56.15 Carousel.home.seek 29 @19:27:56.68 Air.in :unlocking @19:27:56.89 Air.seek :clear @19:27:58.35 Lysis1.in :unlocking @19:27:58.61 Lysis1.seek :clear @19:27:58.68 Plunge.in :unlocking @19:27:58.95 Plunge.seek :clear @19:28:00.15 Carousel.seek 30 @19:28:00.78 Lysis1.in :fast @19:28:00.93 Lysis1.seek :locked @19:28:03.63 Plunge.in :default @19:28:03.81 Plunge.seek :locked @19:28:04.08 Air.in :fast @19:28:04.23 Air.seek :locked @19:28:05.86 Carousel.reconfigure :locked @19:28:06.12 Powering cartridge bus @19:28:08.79 Gate.setIR 1750,5000,0,6 @19:28:08.93 Gate.setIR 0,5000,0,6 @19:28:09.07 PuckHeater.configure :default #ambient 24.6C @19:28:09.22 Gate.power :heat,:ON,limit to 5000mA @19:28:11.94 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @19:28:12.07 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:28:12.12 Vacuuming Vent Top cartridge 30 for 30 seconds @19:28:12.23 Air.in :default @19:28:12.39 Air.seek :evac @19:28:25.52 Toroid.in :hold @19:28:25.70 Toroid.seek :cartridge @19:28:27.62 Toroid.in :default @19:28:27.80 Toroid.seek :clear @19:28:29.68 Polling Can every 2:00 @19:28:30.23 PuckHeater.configure :default #ambient 24.6C @19:28:30.37 PuckHeater.seek 40.9C,1:00 @19:28:51.73 PuckHeater.hold 3 seconds,40.9C @19:28:55.00 delayUntil "19:28:58.72" @19:28:58.83 Toroid.in :hold @19:28:58.98 Toroid.seek :bypass @19:29:00.70 Toroid.in :default @19:29:00.85 Toroid.seek :clear @19:29:02.54 delay 2 seconds @19:29:04.56 Air.seek :full @19:29:19.27 Gate.power :heat,:OFF @19:29:19.36 Gate.power :cartridge,:OFF @19:29:19.51 Carousel.in :default @19:29:19.76 Carousel.home.seek 30 @19:29:20.27 Air.in :unlocking @19:29:20.48 Air.seek :clear @19:29:21.58 Lysis1.in :unlocking @19:29:21.87 Lysis1.seek :clear @19:29:21.95 Plunge.in :unlocking @19:29:22.22 Plunge.seek :clear @19:29:23.41 Carousel.seek 31 @19:29:24.04 Lysis1.in :fast @19:29:24.20 Lysis1.seek :locked @19:29:26.92 Plunge.in :default @19:29:27.08 Plunge.seek :locked @19:29:27.33 Air.in :fast @19:29:27.48 Air.seek :locked @19:29:29.11 Carousel.reconfigure :locked @19:29:29.40 Powering cartridge bus @19:29:32.07 Gate.setIR 1750,5000,0,6 @19:29:32.21 Gate.setIR 0,5000,0,6 @19:29:32.34 Gate.power :cartridge,:OFF @19:29:32.97 Mounted archive Cartridge @19:29:33.10 Vacuuming Solid Top cartridge 31 for 30 seconds @19:29:33.28 Toroid.in :hold @19:29:33.44 Toroid.seek :cartridge @19:29:36.01 Toroid.in :default @19:29:36.26 Toroid.seek :clear @19:29:38.14 delay 30 seconds @19:30:08.27 Toroid.in :hold @19:30:08.47 Toroid.seek :bypass @19:30:10.18 Toroid.in :default @19:30:10.33 Toroid.seek :clear @19:30:12.24 Carousel.in :default @19:30:12.46 Carousel.home.seek 31 @19:30:13.01 Air.in :unlocking @19:30:13.22 Air.seek :clear @19:30:14.68 Lysis1.in :unlocking @19:30:14.95 Lysis1.seek :clear @19:30:15.03 Plunge.in :unlocking @19:30:15.31 Plunge.seek :clear @19:30:16.51 Carousel.seek 32 @19:30:17.15 Lysis1.in :fast @19:30:17.30 Lysis1.seek :locked @19:30:20.00 Plunge.in :default @19:30:20.16 Plunge.seek :locked @19:30:20.41 Air.in :fast @19:30:20.56 Air.seek :locked @19:30:22.20 Carousel.reconfigure :locked @19:30:22.47 Powering cartridge bus @19:30:25.14 Gate.setIR 1750,5000,0,6 @19:30:25.28 Gate.setIR 0,5000,0,6 @19:30:25.42 PuckHeater.configure :default #ambient 24.6C @19:30:25.57 Gate.power :heat,:ON,limit to 5000mA @19:30:26.28 Mounted lysis Cartridge board#Q05052 @19:30:26.39 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:30:26.46 Vacuuming Vent Top cartridge 32 for 30 seconds @19:30:26.51 Air.in :default @19:30:26.71 Air.seek :evac @19:30:39.84 Toroid.in :hold @19:30:40.00 Toroid.seek :cartridge @19:30:41.90 Toroid.in :default @19:30:42.05 Toroid.seek :clear @19:30:43.94 Polling Can every 2:00 @19:30:44.49 PuckHeater.configure :default #ambient 24.5C @19:30:44.63 PuckHeater.seek 40.9C,1:00 @19:31:07.03 PuckHeater.hold 3 seconds,40.9C @19:31:10.29 delayUntil "19:31:13.02" @19:31:13.13 Toroid.in :hold @19:31:13.29 Toroid.seek :bypass @19:31:15.01 Toroid.in :default @19:31:15.17 Toroid.seek :clear @19:31:16.86 delay 2 seconds @19:31:18.89 Air.seek :full @19:31:33.58 Gate.power :heat,:OFF @19:31:33.65 Gate.power :cartridge,:OFF @19:31:33.87 Carousel.in :default @19:31:34.09 Carousel.home.seek 32 @19:31:34.62 Air.in :unlocking @19:31:34.82 Air.seek :clear @19:31:35.92 Lysis1.in :unlocking @19:31:36.17 Lysis1.seek :clear @19:31:36.27 Plunge.in :unlocking @19:31:36.52 Plunge.seek :clear @19:31:37.74 Carousel.seek 33 @19:31:38.39 Lysis1.in :fast @19:31:38.54 Lysis1.seek :locked @19:31:41.26 Plunge.in :default @19:31:41.44 Plunge.seek :locked @19:31:41.70 Air.in :fast @19:31:41.86 Air.seek :locked @19:31:43.50 Carousel.reconfigure :locked @19:31:43.79 Powering cartridge bus @19:31:46.46 Gate.setIR 1750,5000,0,6 @19:31:46.60 Gate.setIR 0,5000,0,6 @19:31:46.74 Gate.power :cartridge,:OFF @19:31:47.37 Mounted archive Cartridge @19:31:47.49 Vacuuming Solid Top cartridge 33 for 30 seconds @19:31:48.46 Toroid.in :hold @19:31:48.61 Toroid.seek :cartridge @19:31:50.52 Toroid.in :default @19:31:50.67 Toroid.seek :clear @19:31:52.55 delay 30 seconds @19:32:22.68 Toroid.in :hold @19:32:22.88 Toroid.seek :bypass @19:32:24.59 Toroid.in :default @19:32:24.74 Toroid.seek :clear @19:32:26.66 Carousel.in :default @19:32:26.88 Carousel.home.seek 33 @19:32:27.40 Air.in :unlocking @19:32:27.64 Air.seek :clear @19:32:29.10 Lysis1.in :unlocking @19:32:29.32 Lysis1.seek :clear @19:32:29.51 Plunge.in :unlocking @19:32:29.70 Plunge.seek :clear @19:32:30.87 Carousel.seek 34 @19:32:31.51 Lysis1.in :fast @19:32:31.69 Lysis1.seek :locked @19:32:34.40 Plunge.in :default @19:32:34.62 Plunge.seek :locked @19:32:34.81 Air.in :fast @19:32:34.97 Air.seek :locked @19:32:36.60 Carousel.reconfigure :locked @19:32:36.88 Powering cartridge bus @19:32:39.55 Gate.setIR 1750,5000,0,6 @19:32:39.69 Gate.setIR 0,5000,0,6 @19:32:39.84 PuckHeater.configure :default #ambient 24.5C @19:32:39.98 Gate.power :heat,:ON,limit to 5000mA @19:32:40.68 Mounted lysis Cartridge board#Q05066 @19:32:40.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:32:40.87 Vacuuming Vent Top cartridge 34 for 30 seconds @19:32:40.92 Air.in :default @19:32:41.12 Air.seek :evac @19:32:54.27 Toroid.in :hold @19:32:54.43 Toroid.seek :cartridge @19:32:56.32 Toroid.in :default @19:32:56.47 Toroid.seek :clear @19:32:58.35 Polling Can every 2:00 @19:32:58.91 PuckHeater.configure :default #ambient 24.6C @19:32:59.04 PuckHeater.seek 40.9C,1:00 @19:33:20.42 PuckHeater.hold 3 seconds,40.9C @19:33:23.67 delayUntil "19:33:27.46" @19:33:27.57 Toroid.in :hold @19:33:27.76 Toroid.seek :bypass @19:33:29.48 Toroid.in :default @19:33:29.67 Toroid.seek :clear @19:33:31.37 delay 2 seconds @19:33:33.40 Air.seek :full @19:33:48.09 Gate.power :heat,:OFF @19:33:48.12 Gate.power :cartridge,:OFF @19:33:48.33 Carousel.in :default @19:33:48.53 Carousel.home.seek 34 @19:33:49.07 Air.in :unlocking @19:33:49.31 Air.seek :clear @19:33:50.46 Lysis1.in :unlocking @19:33:50.67 Lysis1.seek :clear @19:33:50.89 Plunge.in :unlocking @19:33:51.06 Plunge.seek :clear @19:33:52.23 Carousel.seek 35 @19:33:52.86 Lysis1.in :fast @19:33:53.02 Lysis1.seek :locked @19:33:55.77 Plunge.in :default @19:33:55.94 Plunge.seek :locked @19:33:56.18 Air.in :fast @19:33:56.33 Air.seek :locked @19:33:57.98 Carousel.reconfigure :locked @19:33:58.26 Powering cartridge bus @19:34:00.92 Gate.setIR 1750,5000,0,6 @19:34:01.06 Gate.setIR 0,5000,0,6 @19:34:02.02 Gate.power :cartridge,:OFF @19:34:02.64 Mounted archive Cartridge @19:34:02.76 Vacuuming Solid Top cartridge 35 for 30 seconds @19:34:02.94 Toroid.in :hold @19:34:03.09 Toroid.seek :cartridge @19:34:05.02 Toroid.in :default @19:34:05.17 Toroid.seek :clear @19:34:07.07 delay 30 seconds @19:34:37.20 Toroid.in :hold @19:34:37.35 Toroid.seek :bypass @19:34:39.06 Toroid.in :default @19:34:39.20 Toroid.seek :clear @19:34:41.14 Carousel.in :default @19:34:41.36 Carousel.home.seek 35 @19:34:41.93 Air.in :unlocking @19:34:42.19 Air.seek :clear @19:34:43.71 Lysis1.in :unlocking @19:34:43.98 Lysis1.seek :clear @19:34:44.06 Plunge.in :unlocking @19:34:44.32 Plunge.seek :clear @19:34:45.54 Carousel.seek 36 @19:34:46.22 Lysis1.in :fast @19:34:46.36 Lysis1.seek :locked @19:34:49.07 Plunge.in :default @19:34:49.26 Plunge.seek :locked @19:34:49.49 Air.in :fast @19:34:49.66 Air.seek :locked @19:34:51.32 Carousel.reconfigure :locked @19:34:51.58 Powering cartridge bus @19:34:54.25 Gate.setIR 1750,5000,0,6 @19:34:54.39 Gate.setIR 0,5000,0,6 @19:34:54.52 PuckHeater.configure :default #ambient 24.6C @19:34:54.67 Gate.power :heat,:ON,limit to 5000mA @19:34:55.38 Mounted lysis Cartridge board#Q05045 @19:34:55.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:34:55.58 Vacuuming Vent Top cartridge 36 for 30 seconds @19:34:55.71 Air.in :default @19:34:55.85 Air.seek :evac @19:35:09.00 Toroid.in :hold @19:35:09.15 Toroid.seek :cartridge @19:35:11.05 Toroid.in :default @19:35:11.20 Toroid.seek :clear @19:35:13.87 Polling Can every 2:00 @19:35:14.43 PuckHeater.configure :default #ambient 24.5C @19:35:14.57 PuckHeater.seek 40.9C,1:00 @19:35:36.25 PuckHeater.hold 3 seconds,40.9C @19:35:39.52 delayUntil "19:35:42.17" @19:35:42.28 Toroid.in :hold @19:35:42.43 Toroid.seek :bypass @19:35:44.14 Toroid.in :default @19:35:44.29 Toroid.seek :clear @19:35:45.97 delay 2 seconds @19:35:48.00 Air.seek :full @19:36:02.70 Gate.power :heat,:OFF @19:36:02.78 Gate.power :cartridge,:OFF @19:36:02.95 Carousel.in :default @19:36:03.16 Carousel.home.seek 36 @19:36:03.71 Air.in :unlocking @19:36:03.93 Air.seek :clear @19:36:05.04 Lysis1.in :unlocking @19:36:05.30 Lysis1.seek :clear @19:36:05.37 Plunge.in :unlocking @19:36:05.64 Plunge.seek :clear @19:36:06.83 Carousel.seek 37 @19:36:07.46 Lysis1.in :fast @19:36:07.64 Lysis1.seek :locked @19:36:10.37 Plunge.in :default @19:36:10.52 Plunge.seek :locked @19:36:10.76 Air.in :fast @19:36:10.93 Air.seek :locked @19:36:12.56 Carousel.reconfigure :locked @19:36:12.83 Powering cartridge bus @19:36:15.77 Gate.setIR 1750,5000,0,6 @19:36:15.91 Gate.setIR 0,5000,0,6 @19:36:16.05 Gate.power :cartridge,:OFF @19:36:16.67 Mounted archive Cartridge @19:36:16.79 Vacuuming Solid Top cartridge 37 for 30 seconds @19:36:16.98 Toroid.in :hold @19:36:17.13 Toroid.seek :cartridge @19:36:19.05 Toroid.in :default @19:36:19.19 Toroid.seek :clear @19:36:21.07 delay 30 seconds @19:36:51.20 Toroid.in :hold @19:36:51.34 Toroid.seek :bypass @19:36:53.05 Toroid.in :default @19:36:53.20 Toroid.seek :clear @19:36:55.13 Carousel.in :default @19:36:55.35 Carousel.home.seek 37 @19:36:55.93 Air.in :unlocking @19:36:56.15 Air.seek :clear @19:36:57.59 Lysis1.in :unlocking @19:36:57.91 Lysis1.seek :clear @19:36:57.99 Plunge.in :unlocking @19:36:58.27 Plunge.seek :clear @19:36:59.47 Carousel.seek 38 @19:37:00.11 Lysis1.in :fast @19:37:00.25 Lysis1.seek :locked @19:37:02.95 Plunge.in :default @19:37:03.14 Plunge.seek :locked @19:37:03.37 Air.in :fast @19:37:03.52 Air.seek :locked @19:37:05.21 Carousel.reconfigure :locked @19:37:05.48 Powering cartridge bus @19:37:08.17 Gate.setIR 1750,5000,0,6 @19:37:08.30 Gate.setIR 0,5000,0,6 @19:37:08.43 PuckHeater.configure :default #ambient 24.5C @19:37:08.59 Gate.power :heat,:ON,limit to 5000mA @19:37:09.32 Mounted lysis Cartridge board#Q05034 @19:37:09.44 Using this cartridge's heat loss curve for HeatingAccelInCarousel @19:37:09.51 Vacuuming Vent Top cartridge 38 for 30 seconds @19:37:09.57 Air.in :default @19:37:09.82 Air.seek :evac @19:37:22.94 Toroid.in :hold @19:37:23.08 Toroid.seek :cartridge @19:37:24.98 Toroid.in :default @19:37:25.90 Toroid.seek :clear @19:37:27.77 Polling Can every 2:00 @19:37:28.33 PuckHeater.seek 41.2C,1:00 @19:37:50.00 PuckHeater.hold 3 seconds,41.2C @19:37:53.32 delayUntil "19:37:56.10" @19:37:56.21 Toroid.in :hold @19:37:56.35 Toroid.seek :bypass @19:37:58.07 Toroid.in :default @19:37:58.21 Toroid.seek :clear @19:37:59.90 delay 2 seconds @19:38:01.92 Air.seek :full @19:38:16.62 Gate.power :heat,:OFF @19:38:16.65 Gate.power :cartridge,:OFF @19:38:16.88 Carousel.in :default @19:38:17.09 Carousel.home.seek 38 @19:38:17.59 Air.in :unlocking @19:38:17.84 Air.seek :clear @19:38:18.94 Lysis1.in :unlocking @19:38:19.17 Lysis1.seek :clear @19:38:19.36 Plunge.in :unlocking @19:38:19.51 Plunge.seek :clear @19:38:20.69 Carousel.seek 39 @19:38:21.32 Lysis1.in :fast @19:38:21.48 Lysis1.seek :locked @19:38:24.19 Plunge.in :default @19:38:24.34 Plunge.seek :locked @19:38:24.60 Air.in :fast @19:38:24.76 Air.seek :locked @19:38:26.39 Carousel.reconfigure :locked @19:38:27.43 Powering cartridge bus @19:38:30.10 Gate.setIR 1750,5000,0,6 @19:38:30.24 Gate.setIR 0,5000,0,6 @19:38:30.38 Gate.power :cartridge,:OFF @19:38:31.02 Mounted archive Cartridge @19:38:31.14 Vacuuming Solid Top cartridge 39 for 30 seconds @19:38:31.34 Toroid.in :hold @19:38:31.49 Toroid.seek :cartridge @19:38:33.40 Toroid.in :default @19:38:33.54 Toroid.seek :clear @19:38:35.47 delay 30 seconds @19:39:05.60 Toroid.in :hold @19:39:05.84 Toroid.seek :bypass @19:39:07.56 Toroid.in :default @19:39:07.75 Toroid.seek :clear @19:39:09.69 Carousel.in :default @19:39:09.92 Carousel.home.seek 39 @19:39:10.46 Air.in :unlocking @19:39:10.71 Air.seek :clear @19:39:12.24 Lysis1.in :unlocking @19:39:12.49 Lysis1.seek :clear @19:39:12.67 Plunge.in :unlocking @19:39:12.85 Plunge.seek :clear @19:39:14.03 Carousel.seek 40 @19:39:14.65 Lysis1.in :fast @19:39:14.79 Lysis1.seek :locked @19:39:17.51 Plunge.in :default @19:39:17.73 Plunge.seek :locked @19:39:17.94 Air.in :fast @19:39:18.09 Air.seek :locked @19:39:19.73 Carousel.reconfigure :locked @19:39:19.99 Powering cartridge bus @19:39:22.66 Gate.setIR 1750,5000,0,6 @19:39:22.81 Gate.setIR 0,5000,0,6 @19:39:22.96 PuckHeater.configure :default #ambient 24.5C @19:39:23.10 Gate.power :heat,:ON,limit to 5000mA @19:39:23.81 Mounted lysis Cartridge board#Q05058 @19:39:23.92 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:39:23.99 Vacuuming Vent Top cartridge 40 for 30 seconds @19:39:24.04 Air.in :default @19:39:24.23 Air.seek :evac @19:39:37.35 Toroid.in :hold @19:39:38.27 Toroid.seek :cartridge @19:39:40.20 Toroid.in :default @19:39:40.35 Toroid.seek :clear @19:39:42.24 Polling Can every 2:00 @19:39:42.80 PuckHeater.configure :default #ambient 24.5C @19:39:42.94 PuckHeater.seek 40.9C,1:00 @19:40:04.57 PuckHeater.hold 3 seconds,40.9C @19:40:07.80 delayUntil "19:40:11.29" @19:40:11.40 Toroid.in :hold @19:40:11.56 Toroid.seek :bypass @19:40:13.31 Toroid.in :default @19:40:13.46 Toroid.seek :clear @19:40:15.15 delay 2 seconds @19:40:17.18 Air.seek :full @19:40:31.88 Gate.power :heat,:OFF @19:40:31.91 Gate.power :cartridge,:OFF @19:40:32.12 Carousel.in :default @19:40:32.34 Carousel.home.seek 40 @19:40:32.85 Air.in :unlocking @19:40:33.06 Air.seek :clear @19:40:34.14 Lysis1.in :unlocking @19:40:34.35 Lysis1.seek :clear @19:40:34.55 Plunge.in :unlocking @19:40:34.70 Plunge.seek :clear @19:40:35.88 Carousel.seek 41 @19:40:36.51 Lysis1.in :fast @19:40:36.66 Lysis1.seek :locked @19:40:39.33 Plunge.in :default @19:40:39.52 Plunge.seek :locked @19:40:39.77 Air.in :fast @19:40:40.57 Air.seek :locked @19:40:42.33 Carousel.reconfigure :locked @19:40:42.60 Powering cartridge bus @19:40:45.27 Gate.setIR 1750,5000,0,6 @19:40:45.41 Gate.setIR 0,5000,0,6 @19:40:45.55 Gate.power :cartridge,:OFF @19:40:46.21 Mounted archive Cartridge @19:40:46.33 Vacuuming Solid Top cartridge 41 for 30 seconds @19:40:46.50 Toroid.in :hold @19:40:46.65 Toroid.seek :cartridge @19:40:48.57 Toroid.in :default @19:40:48.72 Toroid.seek :clear @19:40:50.59 delay 30 seconds @19:41:20.71 Toroid.in :hold @19:41:20.87 Toroid.seek :bypass @19:41:22.58 Toroid.in :default @19:41:22.73 Toroid.seek :clear @19:41:24.67 Carousel.in :default @19:41:24.89 Carousel.home.seek 41 @19:41:25.43 Air.in :unlocking @19:41:25.68 Air.seek :clear @19:41:27.12 Lysis1.in :unlocking @19:41:27.35 Lysis1.seek :clear @19:41:27.52 Plunge.in :unlocking @19:41:27.74 Plunge.seek :clear @19:41:28.92 Carousel.seek 42 @19:41:29.55 Lysis1.in :fast @19:41:29.73 Lysis1.seek :locked @19:41:32.45 Plunge.in :default @19:41:32.66 Plunge.seek :locked @19:41:32.87 Air.in :fast @19:41:33.02 Air.seek :locked @19:41:34.65 Carousel.reconfigure :locked @19:41:34.91 Powering cartridge bus @19:41:37.58 Gate.setIR 1750,5000,0,6 @19:41:37.73 Gate.setIR 0,5000,0,6 @19:41:37.87 PuckHeater.configure :default #ambient 24.5C @19:41:38.03 Gate.power :heat,:ON,limit to 5000mA @19:41:38.72 Mounted lysis Cartridge board#Q05064 @19:41:38.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:41:38.91 Vacuuming Vent Top cartridge 42 for 30 seconds @19:41:38.97 Air.in :default @19:41:39.86 Air.seek :evac @19:41:53.10 Toroid.in :hold @19:41:53.26 Toroid.seek :cartridge @19:41:55.15 Toroid.in :default @19:41:55.31 Toroid.seek :clear @19:41:57.20 Polling Can every 2:00 @19:41:57.76 PuckHeater.configure :default #ambient 24.5C @19:41:57.90 PuckHeater.seek 40.9C,1:00 @19:42:19.57 PuckHeater.hold 3 seconds,40.9C @19:42:22.89 delayUntil "19:42:26.28" @19:42:26.39 Toroid.in :hold @19:42:26.56 Toroid.seek :bypass @19:42:28.28 Toroid.in :default @19:42:28.44 Toroid.seek :clear @19:42:30.12 delay 2 seconds @19:42:32.15 Air.seek :full @19:42:46.85 Gate.power :heat,:OFF @19:42:46.92 Gate.power :cartridge,:OFF @19:42:47.09 Carousel.in :default @19:42:47.31 Carousel.home.seek 42 @19:42:47.88 Air.in :unlocking @19:42:48.08 Air.seek :clear @19:42:49.19 Lysis1.in :unlocking @19:42:49.45 Lysis1.seek :clear @19:42:49.66 Plunge.in :unlocking @19:42:49.84 Plunge.seek :clear @19:42:51.01 Carousel.seek 43 @19:42:51.68 Lysis1.in :fast @19:42:51.84 Lysis1.seek :locked @19:42:54.57 Plunge.in :default @19:42:55.50 Plunge.seek :locked @19:42:55.78 Air.in :fast @19:42:55.95 Air.seek :locked @19:42:57.59 Carousel.reconfigure :locked @19:42:57.90 Powering cartridge bus @19:43:00.57 Gate.setIR 1750,5000,0,6 @19:43:00.71 Gate.setIR 0,5000,0,6 @19:43:00.87 Gate.power :cartridge,:OFF @19:43:01.50 Mounted archive Cartridge @19:43:01.66 Vacuuming Solid Top cartridge 43 for 30 seconds @19:43:01.85 Toroid.in :hold @19:43:02.00 Toroid.seek :cartridge @19:43:03.92 Toroid.in :default @19:43:04.07 Toroid.seek :clear @19:43:05.95 delay 30 seconds @19:43:36.08 Toroid.in :hold @19:43:36.24 Toroid.seek :bypass @19:43:37.94 Toroid.in :default @19:43:38.10 Toroid.seek :clear @19:43:40.05 Carousel.in :default @19:43:40.28 Carousel.home.seek 43 @19:43:40.83 Air.in :unlocking @19:43:41.06 Air.seek :clear @19:43:42.50 Lysis1.in :unlocking @19:43:42.71 Lysis1.seek :clear @19:43:42.93 Plunge.in :unlocking @19:43:43.09 Plunge.seek :clear @19:43:44.27 Carousel.seek 44 @19:43:44.89 Lysis1.in :fast @19:43:45.04 Lysis1.seek :locked @19:43:47.75 Plunge.in :default @19:43:47.91 Plunge.seek :locked @19:43:48.15 Air.in :fast @19:43:48.31 Air.seek :locked @19:43:49.94 Carousel.reconfigure :locked @19:43:50.20 Powering cartridge bus @19:43:52.87 Gate.setIR 1750,5000,0,6 @19:43:53.01 Gate.setIR 0,5000,0,6 @19:43:53.16 PuckHeater.configure :default #ambient 24.5C @19:43:53.31 Gate.power :heat,:ON,limit to 5000mA @19:43:54.80 Mounted lysis Cartridge board#Q05038 @19:43:54.91 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:43:54.99 Vacuuming Vent Top cartridge 44 for 30 seconds @19:43:55.09 Air.in :default @19:43:55.24 Air.seek :evac @19:44:08.38 Toroid.in :hold @19:44:08.54 Toroid.seek :cartridge @19:44:10.44 Toroid.in :default @19:44:10.61 Toroid.seek :clear @19:44:12.50 Polling Can every 2:00 @19:44:13.06 PuckHeater.configure :default #ambient 24.5C @19:44:13.19 PuckHeater.seek 40.9C,1:00 @19:44:34.88 PuckHeater.hold 3 seconds,40.9C @19:44:38.16 delayUntil "19:44:41.56" @19:44:41.70 Toroid.in :hold @19:44:41.85 Toroid.seek :bypass @19:44:43.57 Toroid.in :default @19:44:43.75 Toroid.seek :clear @19:44:45.44 delay 2 seconds @19:44:47.47 Air.seek :full @19:45:02.18 Gate.power :heat,:OFF @19:45:02.21 Gate.power :cartridge,:OFF @19:45:02.43 Carousel.in :default @19:45:02.64 Carousel.home.seek 44 @19:45:03.16 Air.in :unlocking @19:45:03.41 Air.seek :clear @19:45:04.53 Lysis1.in :unlocking @19:45:04.74 Lysis1.seek :clear @19:45:04.96 Plunge.in :unlocking @19:45:05.12 Plunge.seek :clear @19:45:06.29 Carousel.seek 45 @19:45:06.92 Lysis1.in :fast @19:45:07.07 Lysis1.seek :locked @19:45:10.58 Plunge.in :default @19:45:10.79 Plunge.seek :locked @19:45:10.98 Air.in :fast @19:45:11.13 Air.seek :locked @19:45:12.79 Carousel.reconfigure :locked @19:45:13.05 Powering cartridge bus @19:45:15.74 Gate.setIR 1750,5000,0,6 @19:45:15.88 Gate.setIR 0,5000,0,6 @19:45:16.12 Gate.power :cartridge,:OFF @19:45:16.75 Mounted archive Cartridge @19:45:16.88 Vacuuming Solid Top cartridge 45 for 30 seconds @19:45:17.07 Toroid.in :hold @19:45:17.23 Toroid.seek :cartridge @19:45:19.13 Toroid.in :default @19:45:19.28 Toroid.seek :clear @19:45:21.16 delay 30 seconds @19:45:51.29 Toroid.in :hold @19:45:51.46 Toroid.seek :bypass @19:45:53.16 Toroid.in :default @19:45:53.32 Toroid.seek :clear @19:45:55.25 Carousel.in :default @19:45:55.47 Carousel.home.seek 45 @19:45:56.01 Air.in :unlocking @19:45:56.31 Air.seek :clear @19:45:57.77 Lysis1.in :unlocking @19:45:58.02 Lysis1.seek :clear @19:45:58.08 Plunge.in :unlocking @19:45:58.34 Plunge.seek :clear @19:45:59.55 Carousel.seek 46 @19:46:00.21 Lysis1.in :fast @19:46:00.36 Lysis1.seek :locked @19:46:03.06 Plunge.in :default @19:46:03.25 Plunge.seek :locked @19:46:03.46 Air.in :fast @19:46:03.61 Air.seek :locked @19:46:05.29 Carousel.reconfigure :locked @19:46:05.55 Powering cartridge bus @19:46:08.23 Gate.setIR 1750,5000,0,6 @19:46:08.37 Gate.setIR 0,5000,0,6 @19:46:09.27 PuckHeater.configure :default #ambient 24.5C @19:46:09.42 Gate.power :heat,:ON,limit to 5000mA @19:46:10.13 Mounted lysis Cartridge board#Q05076 @19:46:10.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:46:10.33 Vacuuming Vent Top cartridge 46 for 30 seconds @19:46:10.41 Air.in :default @19:46:10.56 Air.seek :evac @19:46:23.72 Toroid.in :hold @19:46:23.88 Toroid.seek :cartridge @19:46:25.78 Toroid.in :default @19:46:25.94 Toroid.seek :clear @19:46:27.83 Polling Can every 2:00 @19:46:28.39 PuckHeater.configure :default #ambient 24.5C @19:46:28.53 PuckHeater.seek 40.9C,1:00 @19:46:33.32 -> Can Can@19:46:28, 24.5C, 33% humidity, 14.2psia, 14.987V, 0.415A, 5.358Ah, 6.21W @19:46:50.18 PuckHeater.hold 3 seconds,40.9C @19:46:53.37 delayUntil "19:46:56.90" @19:46:57.01 Toroid.in :hold @19:46:57.16 Toroid.seek :bypass @19:46:58.89 Toroid.in :default @19:46:59.04 Toroid.seek :clear @19:47:00.74 delay 2 seconds @19:47:02.76 Air.seek :full @19:47:17.46 Gate.power :heat,:OFF @19:47:17.49 Gate.power :cartridge,:OFF @19:47:17.74 Carousel.in :default @19:47:17.95 Carousel.home.seek 46 @19:47:18.48 Air.in :unlocking @19:47:18.68 Air.seek :clear @19:47:19.77 Lysis1.in :unlocking @19:47:19.95 Lysis1.seek :clear @19:47:20.17 Plunge.in :unlocking @19:47:20.34 Plunge.seek :clear @19:47:21.52 Carousel.seek 47 @19:47:22.69 Lysis1.in :fast @19:47:22.83 Lysis1.seek :locked @19:47:25.54 Plunge.in :default @19:47:25.74 Plunge.seek :locked @19:47:26.02 Air.in :fast @19:47:26.17 Air.seek :locked @19:47:27.84 Carousel.reconfigure :locked @19:47:28.10 Powering cartridge bus @19:47:30.79 Gate.setIR 1750,5000,0,6 @19:47:30.93 Gate.setIR 0,5000,0,6 @19:47:31.09 Gate.power :cartridge,:OFF @19:47:31.70 Mounted archive Cartridge @19:47:31.83 Vacuuming Solid Top cartridge 47 for 30 seconds @19:47:32.02 Toroid.in :hold @19:47:32.18 Toroid.seek :cartridge @19:47:34.09 Toroid.in :default @19:47:34.24 Toroid.seek :clear @19:47:36.13 delay 30 seconds @19:48:06.25 Toroid.in :hold @19:48:06.41 Toroid.seek :bypass @19:48:08.12 Toroid.in :default @19:48:08.26 Toroid.seek :clear @19:48:10.20 Carousel.in :default @19:48:10.42 Carousel.home.seek 47 @19:48:10.95 Air.in :unlocking @19:48:11.17 Air.seek :clear @19:48:12.61 Lysis1.in :unlocking @19:48:12.84 Lysis1.seek :clear @19:48:12.92 Plunge.in :unlocking @19:48:13.18 Plunge.seek :clear @19:48:14.40 Carousel.seek 48 @19:48:15.03 Lysis1.in :fast @19:48:15.17 Lysis1.seek :locked @19:48:17.87 Plunge.in :default @19:48:18.03 Plunge.seek :locked @19:48:18.29 Air.in :fast @19:48:18.44 Air.seek :locked @19:48:20.07 Carousel.reconfigure :locked @19:48:20.99 Powering cartridge bus @19:48:23.76 Gate.setIR 1750,5000,0,6 @19:48:23.90 Gate.setIR 0,5000,0,6 @19:48:24.04 PuckHeater.configure :default #ambient 24.5C @19:48:24.19 Gate.power :heat,:ON,limit to 5000mA @19:48:24.99 Mounted lysis Cartridge board#Q05044 @19:48:25.11 Using this cartridge's heat loss curve for HeatingAccelInCarousel @19:48:25.20 Vacuuming Vent Top cartridge 48 for 30 seconds @19:48:25.28 Air.in :default @19:48:25.46 Air.seek :evac @19:48:38.60 Toroid.in :hold @19:48:38.75 Toroid.seek :cartridge @19:48:40.66 Toroid.in :default @19:48:40.81 Toroid.seek :clear @19:48:42.68 Polling Can every 2:00 @19:48:43.24 PuckHeater.configure :default #ambient 24.5C @19:48:43.38 PuckHeater.seek 40.9C,1:00 @19:49:05.07 PuckHeater.hold 3 seconds,40.9C @19:49:08.29 delayUntil "19:49:11.77" @19:49:11.88 Toroid.in :hold @19:49:12.04 Toroid.seek :bypass @19:49:13.76 Toroid.in :default @19:49:13.91 Toroid.seek :clear @19:49:15.59 delay 2 seconds @19:49:17.65 Air.seek :full @19:49:32.34 Gate.power :heat,:OFF @19:49:32.37 Gate.power :cartridge,:OFF @19:49:32.60 Carousel.in :default @19:49:32.83 Carousel.home.seek 48 @19:49:33.36 Air.in :unlocking @19:49:33.58 Air.seek :clear @19:49:34.69 Lysis1.in :unlocking @19:49:34.90 Lysis1.seek :clear @19:49:35.80 Plunge.in :unlocking @19:49:36.04 Plunge.seek :clear @19:49:37.22 Carousel.seek 49 @19:49:37.87 Lysis1.in :fast @19:49:38.03 Lysis1.seek :locked @19:49:40.75 Plunge.in :default @19:49:40.93 Plunge.seek :locked @19:49:41.17 Air.in :fast @19:49:41.32 Air.seek :locked @19:49:42.95 Carousel.reconfigure :locked @19:49:43.22 Powering cartridge bus @19:49:45.91 Gate.setIR 1750,5000,0,6 @19:49:46.05 Gate.setIR 0,5000,0,6 @19:49:46.21 Gate.power :cartridge,:OFF @19:49:46.84 Mounted archive Cartridge @19:49:46.96 Vacuuming Solid Top cartridge 49 for 30 seconds @19:49:47.16 Toroid.in :hold @19:49:47.31 Toroid.seek :cartridge @19:49:49.22 Toroid.in :default @19:49:49.37 Toroid.seek :clear @19:49:51.24 delay 30 seconds @19:50:21.37 Toroid.in :hold @19:50:21.53 Toroid.seek :bypass @19:50:23.26 Toroid.in :default @19:50:23.41 Toroid.seek :clear @19:50:25.33 Carousel.in :default @19:50:25.56 Carousel.home.seek 49 @19:50:26.13 Air.in :unlocking @19:50:26.34 Air.seek :clear @19:50:27.79 Lysis1.in :unlocking @19:50:27.97 Lysis1.seek :clear @19:50:28.21 Plunge.in :unlocking @19:50:28.38 Plunge.seek :clear @19:50:29.56 Carousel.seek 50 @19:50:30.23 Lysis1.in :fast @19:50:30.37 Lysis1.seek :locked @19:50:33.05 Plunge.in :default @19:50:33.24 Plunge.seek :locked @19:50:33.46 Air.in :fast @19:50:34.39 Air.seek :locked @19:50:36.05 Carousel.reconfigure :locked @19:50:36.33 Powering cartridge bus @19:50:39.01 Gate.setIR 1750,5000,0,6 @19:50:39.15 Gate.setIR 0,5000,0,6 @19:50:39.29 PuckHeater.configure :default #ambient 24.5C @19:50:39.46 Gate.power :heat,:ON,limit to 5000mA @19:50:40.09 Mounted lysis Cartridge @19:50:40.17 WARNING: Using default puck temperature sensor calibration @19:50:40.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:50:40.35 Vacuuming Vent Top cartridge 50 for 30 seconds @19:50:40.42 Air.in :default @19:50:40.63 Air.seek :evac @19:50:53.77 Toroid.in :hold @19:50:53.92 Toroid.seek :cartridge @19:50:55.82 Toroid.in :default @19:50:55.98 Toroid.seek :clear @19:50:57.88 Polling Can every 2:00 @19:50:58.45 PuckHeater.seek 40.9C,1:00 @19:51:59.80 Thermal::Error in bill -- Puck Heater could not reach 40.9C in 1:00 @19:55:10.10 -> MainThread.resume @19:55:10.19 Thread::CannotResume in n#2 -- MAIN is not suspended @19:55:15.17 -> MainThread.recover @19:55:15.24 Thread::CannotResume in n#2 -- No recoverable exceptions @19:55:42.45 -> SC Storage Carousel (SC) at 50 @19:57:30.84 Session Ended @20:00:26.04 -> MainThread.recover @20:00:26.10 Thread::CannotResume in n#2 -- No recoverable exceptions @20:04:47.25 Session Begun @20:04:54.83 -> showlog 300 @20:06:09.59 -> progress @20:06:09.75 NoMethodError in brent -- undefined method `asPastString' for nil:NilClass @20:06:32.89 -> progress "bill" @20:06:32.97 NoMethodError in brent -- undefined method `moribund?' for "bill":String @20:06:47.74 -> progress Thread["bill"] >>> Thread["bill"] is dead <<< @16:44:36 Ensuring (60) cartridge(s) open @16:44:41 Sampling 500.0ml @16:59:46 Sample Pump (SP) pressureOutOfBounds at 304.89ml @16:59:50 Waiting up to 9 seconds for vacuum to reduce from 16.3 to 8.0psi @16:59:55 Sample Pump (SP) pressureOutOfBounds at 306.4ml @16:59:56 Waiting up to 11 seconds for vacuum to reduce from 17.3 to 8.0psi @17:00:01 Sample Pump (SP) pressureOutOfBounds at 308.05ml @17:00:02 Waiting up to 10 seconds for vacuum to reduce from 16.6 to 8.0psi @17:09:30 Sampled 500.0ml @17:09:30 Ensuring (60) cartridge(s) open @17:09:34 Waiting for bill to answer "Rinse complete. Disconnect intake water source and attach air filter." @17:33:09 Waiting for bill to answer "Hook up vacuum Flask to to Exhaust line, turn on vacuum, remove Fish in slot 60, push return" @17:54:09 Waiting for bill to answer "replace Fish in slot 60, remove fish in slot 1" @17:55:34 Waiting for bill to answer "replace Fish in slot 1 and release vacuum" @17:56:35 Ensuring all (59) cartridges are bypassed @18:14:04 Vacuuming protocol for dry cartridges @18:14:06 Waiting for bill to answer "Disconnect the intake line. push return" @18:15:48 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @18:15:58 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @18:16:02 Clearing fluid from cartridge-side of Toroid @18:27:18 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @18:54:58 Waiting for bill to answer "Turn the pump on. Push Return" @18:55:04 Vacuuming individual cartridges. @19:51:59 Puck Heater could not reach 40.9C in 1:00 @20:07:46.35 Session Ended @20:08:53.39 -> Cart { Type: :microSPR_phyto, State: :dry, TV: :open, lysed: 1000, lysed2: 1000, was: :standard} @20:09:02.60 -> SC Storage Carousel (SC) at 50 @20:09:14.68 -> Heat Puck Heater at 72.7C @20:09:35.39 -> Heat.status @20:09:49.91 -> can Can@20:09:50, 24.1C, 34% humidity, 14.2psia, 14.975V, 0.408A, 5.539Ah, 6.12W @20:10:02.95 -> Heat.coast @20:10:02.98 PuckHeater.coast Puck Heater at 72.6C @20:10:05.78 -> Heat Puck Heater at 72.7C @20:11:22.22 -> Heat.hold 60, 80 @20:11:22.25 Polling Can every 2:00 @20:11:22.86 PuckHeater.configure :default #ambient 24.1C @20:11:22.98 PuckHeater.hold 1:00,82.8C # @20:11:27.50 -> Heat.status @20:11:34.97 -> Heat Puck Heater at 72.7C @20:11:37.66 -> Heat Puck Heater at 72.7C @20:11:40.59 -> Heat Puck Heater at 72.7C @20:11:47.98 -> Heat Puck Heater at 72.7C @20:11:54.56 -> Heat.coast @20:11:54.59 PuckHeater.coast Puck Heater at 72.7C @20:12:04.10 -> Cart.unlock @20:12:04.19 Gate.power :heat,:OFF @20:12:04.21 Gate.power :cartridge,:OFF @20:12:04.40 Carousel.in :default @20:12:04.59 Carousel.home.seek 50 @20:12:04.95 Toroid.in :hold @20:12:05.07 Toroid.seek :bypass @20:12:06.78 Toroid.in :default @20:12:06.91 Toroid.seek :clear @20:12:08.75 Air.in :unlocking @20:12:08.94 Air.seek :clear @20:12:13.20 Lysis1.in :unlocking @20:12:13.41 Lysis1.seek :clear @20:12:13.56 Plunge.in :unlocking @20:12:13.73 Plunge.seek :clear @20:12:20.36 -> Cart.lock @20:12:20.60 Lysis1.in :fast @20:12:20.70 Lysis1.seek :locked @20:12:23.41 Plunge.in :default @20:12:23.62 Plunge.seek :locked @20:12:23.81 Air.in :fast @20:12:23.93 Air.seek :locked @20:12:25.58 Carousel.reconfigure :locked @20:12:25.83 Powering cartridge bus @20:12:28.49 Gate.setIR 1750,5000,0,6 @20:12:28.63 Gate.setIR 0,5000,0,6 @20:12:28.75 PuckHeater.configure :default #ambient 24.1C @20:12:28.87 Gate.power :heat,:ON,limit to 5000mA @20:12:29.50 Mounted lysis Cartridge @20:12:29.57 WARNING: Using default puck temperature sensor calibration @20:12:29.63 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @20:12:39.18 -> Heat Puck Heater at 72.7C @20:12:58.23 -> Heat.hold 60, 80 @20:12:58.29 Polling Can every 2:00 @20:12:58.86 PuckHeater.configure :default #ambient 24.2C @20:12:58.98 PuckHeater.hold 1:00,82.8C # @20:13:04.76 -> Heat.status @20:13:08.04 -> Heat Puck Heater at 72.7C @20:13:10.58 -> Heat Puck Heater at 72.7C @20:13:21.26 -> Heat.coast @20:13:21.29 PuckHeater.coast Puck Heater at 72.6C @20:13:59.76 -> to 55 @20:14:00.01 Gate.power :heat,:OFF @20:14:00.04 Gate.power :cartridge,:OFF @20:14:00.23 Carousel.in :default @20:14:00.44 Carousel.home.seek 50 @20:14:00.96 Air.in :unlocking @20:14:01.19 Air.seek :clear @20:14:02.65 Lysis1.in :unlocking @20:14:02.88 Lysis1.seek :clear @20:14:03.01 Plunge.in :unlocking @20:14:03.15 Plunge.seek :clear @20:14:04.35 Carousel.seek 55 @20:14:05.78 Lysis1.in :fast @20:14:05.91 Lysis1.seek :locked @20:14:08.60 Plunge.in :default @20:14:08.74 Plunge.seek :locked @20:14:08.96 Air.in :fast @20:14:09.09 Air.seek :locked @20:14:10.72 Carousel.reconfigure :locked @20:14:10.95 Powering cartridge bus @20:14:13.62 Gate.setIR 1750,5000,0,6 @20:14:13.77 Gate.setIR 0,5000,0,6 @20:14:13.89 PuckHeater.configure :default #ambient 24.2C @20:14:14.01 Gate.power :heat,:ON,limit to 5000mA @20:14:14.73 Mounted lysis Cartridge board#Q05063 @20:14:15.28 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @20:14:19.84 -> Heat Puck Heater at 24C @20:14:44.56 -> Heat.hold 15, 40 @20:14:44.60 Polling Can every 2:00 @20:14:45.20 PuckHeater.configure :default #ambient 24.5C @20:14:45.32 PuckHeater.hold 15 seconds,40.9C # @20:14:48.12 -> Heat Puck Heater at 26C @20:14:51.27 -> Heat Puck Heater at 30.9C @20:14:54.27 -> Heat Puck Heater at 32.9C @20:14:58.75 -> Heat Puck Heater at 36C @20:15:00.62 -> Heat Puck Heater at 36.9C @20:15:02.24 -> Heat Puck Heater at 37.3C @20:15:04.23 -> Heat Puck Heater at 35.8C @20:15:06.31 -> Heat Puck Heater at 34.4C @20:15:12.39 -> Heat.to 40 @20:15:12.43 Polling Can every 2:00 @20:15:13.02 PuckHeater.configure :default #ambient 24.5C @20:15:13.14 PuckHeater.seek 40.9C Puck Heater at 39.1C @20:15:31.83 -> Heat.stop @20:15:31.86 PuckHeater.coast Puck Heater at 40.4C @20:15:42.23 -> Cart.unlock @20:15:42.31 Gate.power :heat,:OFF @20:15:42.34 Gate.power :cartridge,:OFF @20:15:42.50 Carousel.in :default @20:15:42.71 Carousel.home.seek 55 @20:15:43.22 Air.in :unlocking @20:15:43.43 Air.seek :clear @20:15:44.92 Lysis1.in :unlocking @20:15:45.15 Lysis1.seek :clear @20:15:45.22 Plunge.in :unlocking @20:15:45.43 Plunge.seek :clear @20:16:34.06 -> list :vacToroid @20:16:34.13 NoMethodError in brent -- undefined method `to_srcRef' for nil:NilClass @20:16:44.61 -> list method :vacToroid def vacToroid wait=30, dryCarts=Cartridge.dry #Vacuums toroid and each (dry) cartridge prior to deployment #Vaccum from both the top and bottom of the cartridge since PB is open. Log.record "Vacuuming protocol for dry cartridges" ESP.ready! prompt "Disconnect the intake line. push return" clearSampleLines Log.record "Clearing fluid from cartridge-side of Toroid" for cart in dryCarts Cartridge.open cart delay 2 Cartridge.bypass end if wait prompt "Turn the pump off & load the cartridges. Press Return when loaded." vacLeakTest wait, dryCarts end end @20:18:12.74 -> Cartidge.dry @20:18:12.81 NameError in brent -- uninitialized constant Cartidge @20:18:17.42 -> Cart.dry [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60] @20:19:51.34 -> list method :vacLeakTest def vacLeakTest wait=30, dryCarts=Cartridge.dry, vacWait=600 #assumes cartridges already loaded and sample loop filled with air #Individal cartridge Leak test ESP.ready! Intake.close PB.open Exhaust.open prompt "Turn the pump on. Push Return" Log.record "Vacuuming individual cartridges." vacCarts wait, dryCarts Log.record "Cartridges under vacuum. Individual Leak Test begins" prompt "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." delay vacWait prompt "if Vacuum dropped in sample bypass then recharge before proceeding" Log.record "move through each cartridge position & record end vacuum" recVac dryCarts Cartridge.unmount prompt "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." end @20:21:24.05 -> exit @20:21:24.08 Session Ended @20:24:01.53 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 52..50 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :dry, lysed: 1000, lysed2: 1000, sensed: :archive, was: :standard}} @20:25:34.30 -> Cartridge.reserve [50..53] 4 @20:25:37.66 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :dry}, 53 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, sensed: :archive, was: :dry}} @20:28:50.14 -> vacToroid 30, 54..60 @20:28:50.17 Vacuuming protocol for dry cartridges @20:28:51.07 Toroid.seek :clear @20:28:51.20 Carousel.in :default @20:28:51.37 Carousel.home.seek 55 @20:28:51.73 Waiting for n to answer "Disconnect the intake line. push return" @20:28:56.08 Answered Disconnect the intake line. push return"" @20:28:56.93 Toroid.seek :clear @20:28:57.06 Carousel.in :default @20:28:57.23 Carousel.home.seek 55 @20:28:57.55 SamplePump.setPosition! 0ml @20:28:57.67 Intake.open @20:28:58.39 SamplePump.seek 50ml @20:29:59.55 Exhaust.close @20:30:00.32 SamplePump.seek 75ml @20:30:31.13 Intake.close @20:30:31.88 Bypass.close @20:30:32.63 Waiting for n to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @20:31:04.79 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @20:31:04.81 Exhaust.open @20:31:05.63 Bypass.open @20:31:06.42 Waiting for n to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @20:31:57.43 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @20:31:57.45 delay 30 seconds @20:32:27.50 Clearing fluid from cartridge-side of Toroid @20:32:27.89 Carousel.seek 54 @20:32:28.35 Toroid.in :hold @20:32:28.46 Toroid.seek :cartridge @20:32:30.34 Toroid.in :default @20:32:30.45 Toroid.seek :clear @20:32:32.30 delay 2 seconds @20:32:34.42 Toroid.in :hold @20:32:34.53 Toroid.seek :bypass @20:32:36.21 Toroid.in :default @20:32:36.33 Toroid.seek :clear @20:32:38.82 Carousel.seek 55 @20:32:39.27 Toroid.in :hold @20:32:39.38 Toroid.seek :cartridge @20:32:41.26 Toroid.in :default @20:32:41.37 Toroid.seek :clear @20:32:43.22 delay 2 seconds @20:32:45.34 Toroid.in :hold @20:32:45.45 Toroid.seek :bypass @20:32:47.13 Toroid.in :default @20:32:47.24 Toroid.seek :clear @20:32:49.19 Carousel.seek 56 @20:32:49.67 Toroid.in :hold @20:32:49.79 Toroid.seek :cartridge @20:32:51.70 Toroid.in :default @20:32:51.81 Toroid.seek :clear @20:32:53.67 delay 2 seconds @20:32:55.79 Toroid.in :hold @20:32:55.91 Toroid.seek :bypass @20:32:57.60 Toroid.in :default @20:32:57.75 Toroid.seek :clear @20:32:59.73 Carousel.seek 57 @20:33:00.19 Toroid.in :hold @20:33:00.30 Toroid.seek :cartridge @20:33:02.18 Toroid.in :default @20:33:02.29 Toroid.seek :clear @20:33:04.13 delay 2 seconds @20:33:06.25 Toroid.in :hold @20:33:06.36 Toroid.seek :bypass @20:33:08.04 Toroid.in :default @20:33:08.16 Toroid.seek :clear @20:33:10.11 Carousel.seek 58 @20:33:10.57 Toroid.in :hold @20:33:10.68 Toroid.seek :cartridge @20:33:12.56 Toroid.in :default @20:33:12.68 Toroid.seek :clear @20:33:14.54 delay 2 seconds @20:33:16.66 Toroid.in :hold @20:33:16.77 Toroid.seek :bypass @20:33:18.45 Toroid.in :default @20:33:18.56 Toroid.seek :clear @20:33:21.01 Carousel.seek 59 @20:33:21.46 Toroid.in :hold @20:33:21.57 Toroid.seek :cartridge @20:33:23.49 Toroid.in :default @20:33:23.60 Toroid.seek :clear @20:33:25.49 delay 2 seconds @20:33:27.60 Toroid.in :hold @20:33:27.76 Toroid.seek :bypass @20:33:29.45 Toroid.in :default @20:33:29.56 Toroid.seek :clear @20:33:31.55 Carousel.seek 60 @20:33:32.03 Toroid.in :hold @20:33:32.15 Toroid.seek :cartridge @20:33:34.02 Toroid.in :default @20:33:34.13 Toroid.seek :clear @20:33:35.97 delay 2 seconds @20:33:38.09 Toroid.in :hold @20:33:38.21 Toroid.seek :bypass @20:33:39.89 Toroid.in :default @20:33:40.00 Toroid.seek :clear @20:33:41.68 Waiting for n to answer "Turn the pump off & load the cartridges. Press Return when loaded." @20:33:53.66 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @20:33:54.51 Toroid.seek :clear @20:33:54.65 Carousel.in :default @20:33:54.81 Carousel.home.seek 60 @20:33:55.12 Waiting for n to answer "Turn the pump on. Push Return" @20:34:00.51 Answered Turn the pump on. Push Return"" @20:34:00.53 Vacuuming individual cartridges. @20:34:01.44 Toroid.seek :clear @20:34:01.59 Carousel.in :default @20:34:01.80 Carousel.home.seek 60 @20:34:02.41 Carousel.seek 54 @20:34:04.04 Lysis1.in :fast @20:34:04.15 Lysis1.seek :locked @20:34:06.93 Plunge.in :default @20:34:07.05 Plunge.seek :locked @20:34:07.26 Air.in :fast @20:34:07.37 Air.seek :locked @20:34:08.99 Carousel.reconfigure :locked @20:34:09.21 Powering cartridge bus @20:34:11.88 Gate.setIR 1750,5000,0,6 @20:34:12.01 Gate.setIR 0,5000,0,6 @20:34:12.13 Gate.power :cartridge,:OFF @20:34:12.72 Mounted archive Cartridge @20:34:12.81 Vacuuming Solid Top cartridge 54 for 30 seconds @20:34:12.96 Toroid.in :hold @20:34:13.07 Toroid.seek :cartridge @20:34:14.96 Toroid.in :default @20:34:15.07 Toroid.seek :clear @20:34:16.92 delay 30 seconds @20:34:47.04 Toroid.in :hold @20:34:47.16 Toroid.seek :bypass @20:34:48.83 Toroid.in :default @20:34:48.95 Toroid.seek :clear @20:34:50.85 Carousel.in :default @20:34:51.04 Carousel.home.seek 54 @20:34:51.55 Air.in :unlocking @20:34:51.78 Air.seek :clear @20:34:53.21 Lysis1.in :unlocking @20:34:53.40 Lysis1.seek :clear @20:34:53.57 Plunge.in :unlocking @20:34:53.71 Plunge.seek :clear @20:34:54.86 Carousel.seek 55 @20:34:55.44 Lysis1.in :fast @20:34:55.56 Lysis1.seek :locked @20:34:58.25 Plunge.in :default @20:34:58.39 Plunge.seek :locked @20:34:58.57 Air.in :fast @20:34:58.67 Air.seek :locked @20:35:00.28 Carousel.reconfigure :locked @20:35:00.50 Powering cartridge bus @20:35:03.16 Gate.setIR 1750,5000,0,6 @20:35:03.29 Gate.setIR 0,5000,0,6 @20:35:03.41 PuckHeater.configure :default #ambient 24.5C @20:35:04.00 Gate.power :heat,:ON,limit to 5000mA @20:35:04.83 Mounted lysis Cartridge board#Q05063 @20:35:04.91 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:35:04.95 Vacuuming Vent Top cartridge 55 for 30 seconds @20:35:05.00 Air.in :default @20:35:05.14 Air.seek :evac @20:35:18.23 Toroid.in :hold @20:35:18.35 Toroid.seek :cartridge @20:35:20.23 Toroid.in :default @20:35:20.34 Toroid.seek :clear @20:35:22.19 Polling Can every 2:00 @20:35:22.75 PuckHeater.configure :default #ambient 24.3C @20:35:22.85 PuckHeater.seek 40.9C,1:00 @20:35:44.43 PuckHeater.hold 3 seconds,40.9C @20:35:47.66 delayUntil "20:35:51.37" @20:35:51.48 Toroid.in :hold @20:35:51.59 Toroid.seek :bypass @20:35:53.32 Toroid.in :default @20:35:53.42 Toroid.seek :clear @20:35:55.08 delay 2 seconds @20:35:57.11 Air.seek :full @20:36:11.84 Gate.power :heat,:OFF @20:36:11.87 Gate.power :cartridge,:OFF @20:36:12.02 Carousel.in :default @20:36:12.21 Carousel.home.seek 55 @20:36:12.70 Air.in :unlocking @20:36:12.91 Air.seek :clear @20:36:14.04 Lysis1.in :unlocking @20:36:14.25 Lysis1.seek :clear @20:36:14.32 Plunge.in :unlocking @20:36:14.51 Plunge.seek :clear @20:36:15.73 Carousel.seek 56 @20:36:16.75 Lysis1.in :fast @20:36:16.97 Lysis1.seek :locked @20:36:19.67 Plunge.in :default @20:36:19.79 Plunge.seek :locked @20:36:19.98 Air.in :fast @20:36:20.09 Air.seek :locked @20:36:21.68 Carousel.reconfigure :locked @20:36:21.92 Powering cartridge bus @20:36:24.60 Gate.setIR 1750,5000,0,6 @20:36:24.76 Gate.setIR 0,5000,0,6 @20:36:24.88 Gate.power :cartridge,:OFF @20:36:25.47 Mounted archive Cartridge @20:36:25.56 Vacuuming Solid Top cartridge 56 for 30 seconds @20:36:25.75 Toroid.in :hold @20:36:25.87 Toroid.seek :cartridge @20:36:27.75 Toroid.in :default @20:36:27.87 Toroid.seek :clear @20:36:29.72 delay 30 seconds @20:36:59.85 Toroid.in :hold @20:36:59.96 Toroid.seek :bypass @20:37:01.66 Toroid.in :default @20:37:01.79 Toroid.seek :clear @20:37:03.70 Carousel.in :default @20:37:03.89 Carousel.home.seek 56 @20:37:04.40 Air.in :unlocking @20:37:04.61 Air.seek :clear @20:37:06.08 Lysis1.in :unlocking @20:37:06.30 Plunge.in :unlocking @20:37:06.33 Lysis1.seek :clear @20:37:06.50 Plunge.seek :clear @20:37:07.81 Carousel.seek 57 @20:37:08.41 Lysis1.in :fast @20:37:08.52 Lysis1.seek :locked @20:37:11.19 Plunge.in :default @20:37:11.34 Plunge.seek :locked @20:37:11.51 Air.in :fast @20:37:11.61 Air.seek :locked @20:37:13.28 Carousel.reconfigure :locked @20:37:13.49 Powering cartridge bus @20:37:16.45 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :dry}, 53 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, sensed: :archive, was: :dry}} @20:37:17.03 Gate.setIR 1750,5000,0,6 @20:37:17.16 Gate.setIR 0,5000,0,6 @20:37:17.27 PuckHeater.configure :default #ambient 24.3C @20:37:17.38 Gate.power :heat,:ON,limit to 5000mA @20:37:18.10 Mounted lysis Cartridge board#Q05048 @20:37:18.17 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:37:18.22 Vacuuming Vent Top cartridge 57 for 30 seconds @20:37:18.28 Air.in :default @20:37:18.40 Air.seek :evac @20:37:31.49 Toroid.in :hold @20:37:31.61 Toroid.seek :cartridge @20:37:33.51 Toroid.in :default @20:37:33.64 Toroid.seek :clear @20:37:35.52 Polling Can every 2:00 @20:37:36.07 PuckHeater.configure :default #ambient 24.4C @20:37:36.17 PuckHeater.seek 40.9C,1:00 @20:37:57.78 PuckHeater.hold 3 seconds,40.9C @20:38:01.05 delayUntil "20:38:04.65" @20:38:04.76 Toroid.in :hold @20:38:04.87 Toroid.seek :bypass @20:38:06.56 Toroid.in :default @20:38:06.68 Toroid.seek :clear @20:38:08.32 delay 2 seconds @20:38:10.35 Air.seek :full @20:38:25.01 Gate.power :heat,:OFF @20:38:25.03 Gate.power :cartridge,:OFF @20:38:25.19 Carousel.in :default @20:38:25.36 Carousel.home.seek 57 @20:38:25.89 Air.in :unlocking @20:38:26.12 Air.seek :clear @20:38:27.26 Lysis1.in :unlocking @20:38:27.47 Lysis1.seek :clear @20:38:27.61 Plunge.in :unlocking @20:38:28.30 Plunge.seek :clear @20:38:29.45 Carousel.seek 58 @20:38:30.05 Lysis1.in :fast @20:38:30.16 Lysis1.seek :locked @20:38:32.83 Plunge.in :default @20:38:32.95 Plunge.seek :locked @20:38:33.15 Air.in :fast @20:38:33.26 Air.seek :locked @20:38:34.88 Carousel.reconfigure :locked @20:38:35.10 Powering cartridge bus @20:38:37.77 Gate.setIR 1750,5000,0,6 @20:38:37.90 Gate.setIR 0,5000,0,6 @20:38:38.01 Gate.power :cartridge,:OFF @20:38:38.59 Mounted archive Cartridge @20:38:38.69 Vacuuming Solid Top cartridge 58 for 30 seconds @20:38:38.83 Toroid.in :hold @20:38:38.95 Toroid.seek :cartridge @20:38:40.82 Toroid.in :default @20:38:40.94 Toroid.seek :clear @20:38:42.79 delay 30 seconds @20:39:12.92 Toroid.in :hold @20:39:13.03 Toroid.seek :bypass @20:39:14.71 Toroid.in :default @20:39:14.82 Toroid.seek :clear @20:39:16.71 Carousel.in :default @20:39:16.89 Carousel.home.seek 58 @20:39:17.39 Air.in :unlocking @20:39:17.56 Air.seek :clear @20:39:19.00 Lysis1.in :unlocking @20:39:19.20 Lysis1.seek :clear @20:39:19.28 Plunge.in :unlocking @20:39:19.46 Plunge.seek :clear @20:39:20.68 Carousel.seek 59 @20:39:21.27 Lysis1.in :fast @20:39:21.38 Lysis1.seek :locked @20:39:24.04 Plunge.in :default @20:39:24.16 Plunge.seek :locked @20:39:24.38 Air.in :fast @20:39:24.48 Air.seek :locked @20:39:26.63 Carousel.reconfigure :locked @20:39:26.86 Powering cartridge bus @20:39:29.52 Gate.setIR 1750,5000,0,6 @20:39:29.66 Gate.setIR 0,5000,0,6 @20:39:29.77 PuckHeater.configure :default #ambient 24.4C @20:39:29.88 Gate.power :heat,:ON,limit to 5000mA @20:39:30.59 Mounted lysis Cartridge board#Q05037 @20:39:30.65 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:39:30.73 Vacuuming Vent Top cartridge 59 for 30 seconds @20:39:30.77 Air.in :default @20:39:30.88 Air.seek :evac @20:39:43.97 Toroid.in :hold @20:39:44.08 Toroid.seek :cartridge @20:39:45.96 Toroid.in :default @20:39:46.07 Toroid.seek :clear @20:39:47.92 Polling Can every 2:00 @20:39:48.48 PuckHeater.configure :default #ambient 24.4C @20:39:48.58 PuckHeater.seek 40.9C,1:00 @20:40:10.16 PuckHeater.hold 3 seconds,40.9C @20:40:13.35 delayUntil "20:40:17.10" @20:40:17.23 Toroid.in :hold @20:40:17.34 Toroid.seek :bypass @20:40:19.02 Toroid.in :default @20:40:19.13 Toroid.seek :clear @20:40:20.79 delay 2 seconds @20:40:22.82 Air.seek :full @20:40:37.48 Gate.power :heat,:OFF @20:40:37.51 Gate.power :cartridge,:OFF @20:40:37.69 Carousel.in :default @20:40:37.86 Carousel.home.seek 59 @20:40:38.35 Air.in :unlocking @20:40:38.56 Air.seek :clear @20:40:39.72 Lysis1.in :unlocking @20:40:40.46 Lysis1.seek :clear @20:40:40.59 Plunge.in :unlocking @20:40:40.71 Plunge.seek :clear @20:40:41.90 Carousel.seek 60 @20:40:42.51 Lysis1.in :fast @20:40:42.62 Lysis1.seek :locked @20:40:45.30 Plunge.in :default @20:40:45.41 Plunge.seek :locked @20:40:45.67 Air.in :fast @20:40:45.79 Air.seek :locked @20:40:47.41 Carousel.reconfigure :locked @20:40:47.68 Powering cartridge bus @20:40:50.34 Gate.setIR 1750,5000,0,6 @20:40:50.48 Gate.setIR 0,5000,0,6 @20:40:50.58 PuckHeater.configure :default #ambient 24.4C @20:40:50.70 Gate.power :heat,:ON,limit to 5000mA @20:40:51.39 Mounted lysis Cartridge board#Q05054 @20:40:51.47 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:40:51.53 Vacuuming Vent Top cartridge 60 for 30 seconds @20:40:51.56 Air.in :default @20:40:51.74 Air.seek :evac @20:41:04.83 Toroid.in :hold @20:41:04.94 Toroid.seek :cartridge @20:41:06.82 Toroid.in :default @20:41:06.93 Toroid.seek :clear @20:41:08.76 Polling Can every 2:00 @20:41:09.32 PuckHeater.configure :default #ambient 24.4C @20:41:09.42 PuckHeater.seek 40.9C,1:00 @20:41:28.90 PuckHeater.hold 3 seconds,40.9C @20:41:32.13 delayUntil "20:41:37.96" @20:41:38.07 Toroid.in :hold @20:41:38.19 Toroid.seek :bypass @20:41:39.85 Toroid.in :default @20:41:39.96 Toroid.seek :clear @20:41:41.64 delay 2 seconds @20:41:43.68 Air.seek :full @20:41:58.16 Gate.power :heat,:OFF @20:41:58.19 Gate.power :cartridge,:OFF @20:41:58.86 Carousel.in :default @20:41:59.05 Carousel.home.seek 60 @20:41:59.54 Air.in :unlocking @20:41:59.74 Air.seek :clear @20:42:00.81 Lysis1.in :unlocking @20:42:01.05 Lysis1.seek :clear @20:42:01.28 Plunge.in :unlocking @20:42:01.40 Plunge.seek :clear @20:42:02.55 Cartridges under vacuum. Individual Leak Test begins @20:42:02.58 Waiting for n to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @20:43:35.39 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @20:43:35.41 delay 10:00 @20:53:35.46 Waiting for n to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @20:54:29.32 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @20:54:29.34 move through each cartridge position & record end vacuum @20:54:29.38 Vacuuming cartridge 54 @20:54:29.80 Carousel.seek 54 @20:54:31.40 Lysis1.in :fast @20:54:31.51 Lysis1.seek :locked @20:54:34.17 Plunge.in :default @20:54:34.36 Plunge.seek :locked @20:54:34.51 Air.in :fast @20:54:34.62 Air.seek :locked @20:54:36.23 Carousel.reconfigure :locked @20:54:36.46 Powering cartridge bus @20:54:39.12 Gate.setIR 1750,5000,0,6 @20:54:39.25 Gate.setIR 0,5000,0,6 @20:54:39.37 Gate.power :cartridge,:OFF @20:54:39.98 Mounted archive Cartridge @20:54:40.16 Toroid.in :hold @20:54:40.28 Toroid.seek :cartridge @20:54:42.16 Toroid.in :default @20:54:42.27 Toroid.seek :clear @20:54:44.12 Waiting for n to answer "record Vacuum" @20:54:57.68 Answered record Vacuum"" @20:54:57.83 Toroid.in :hold @20:54:57.94 Toroid.seek :bypass @20:54:59.64 Toroid.in :default @20:54:59.78 Toroid.seek :clear @20:55:01.45 Waiting for n to answer "recharge if needed" @20:55:17.75 Answered recharge if needed"" @20:55:17.77 Vacuuming cartridge 55 @20:55:18.07 Carousel.in :default @20:55:18.26 Carousel.home.seek 54 @20:55:19.30 Air.in :unlocking @20:55:19.46 Air.seek :clear @20:55:20.86 Lysis1.in :unlocking @20:55:21.18 Lysis1.seek :clear @20:55:21.33 Plunge.in :unlocking @20:55:21.44 Plunge.seek :clear @20:55:22.59 Carousel.seek 55 @20:55:23.20 Lysis1.in :fast @20:55:23.31 Lysis1.seek :locked @20:55:26.00 Plunge.in :default @20:55:26.15 Plunge.seek :locked @20:55:26.31 Air.in :fast @20:55:26.43 Air.seek :locked @20:55:28.05 Carousel.reconfigure :locked @20:55:28.26 Powering cartridge bus @20:55:30.93 Gate.setIR 1750,5000,0,6 @20:55:31.07 Gate.setIR 0,5000,0,6 @20:55:31.18 PuckHeater.configure :default #ambient 24.4C @20:55:31.30 Gate.power :heat,:ON,limit to 5000mA @20:55:32.04 Mounted lysis Cartridge board#Q05063 @20:55:32.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:55:32.28 Toroid.in :hold @20:55:32.41 Toroid.seek :cartridge @20:55:34.28 Toroid.in :default @20:55:34.39 Toroid.seek :clear @20:55:36.25 Waiting for n to answer "record Vacuum" @20:55:37.44 Answered record Vacuum"" @20:55:37.60 Toroid.in :hold @20:55:37.77 Toroid.seek :bypass @20:55:39.44 Toroid.in :default @20:55:39.56 Toroid.seek :clear @20:55:41.24 Waiting for n to answer "recharge if needed" @20:55:52.05 Answered recharge if needed"" @20:55:52.08 Vacuuming cartridge 56 @20:55:52.32 Gate.power :heat,:OFF @20:55:52.34 Gate.power :cartridge,:OFF @20:55:52.50 Carousel.in :default @20:55:52.67 Carousel.home.seek 55 @20:55:53.16 Air.in :unlocking @20:55:53.38 Air.seek :clear @20:55:54.84 Lysis1.in :unlocking @20:55:55.04 Lysis1.seek :clear @20:55:55.19 Plunge.in :unlocking @20:55:55.89 Plunge.seek :clear @20:55:57.05 Carousel.seek 56 @20:55:57.69 Lysis1.in :fast @20:55:57.80 Lysis1.seek :locked @20:56:00.48 Plunge.in :default @20:56:00.65 Plunge.seek :locked @20:56:00.81 Air.in :fast @20:56:00.92 Air.seek :locked @20:56:02.53 Carousel.reconfigure :locked @20:56:02.75 Powering cartridge bus @20:56:05.42 Gate.setIR 1750,5000,0,6 @20:56:05.56 Gate.setIR 0,5000,0,6 @20:56:05.71 Gate.power :cartridge,:OFF @20:56:06.30 Mounted archive Cartridge @20:56:06.48 Toroid.in :hold @20:56:06.58 Toroid.seek :cartridge @20:56:08.46 Toroid.in :default @20:56:08.58 Toroid.seek :clear @20:56:10.44 Waiting for n to answer "record Vacuum" @20:56:16.52 Answered record Vacuum"" @20:56:16.68 Toroid.in :hold @20:56:16.78 Toroid.seek :bypass @20:56:18.46 Toroid.in :default @20:56:18.57 Toroid.seek :clear @20:56:20.23 Waiting for n to answer "recharge if needed" @20:56:20.90 Answered recharge if needed"" @20:56:20.91 Vacuuming cartridge 57 @20:56:21.26 Carousel.in :default @20:56:21.45 Carousel.home.seek 56 @20:56:21.94 Air.in :unlocking @20:56:22.12 Air.seek :clear @20:56:23.50 Lysis1.in :unlocking @20:56:23.76 Lysis1.seek :clear @20:56:23.83 Plunge.in :unlocking @20:56:24.02 Plunge.seek :clear @20:56:25.23 Carousel.seek 57 @20:56:25.85 Lysis1.in :fast @20:56:25.96 Lysis1.seek :locked @20:56:28.65 Plunge.in :default @20:56:28.82 Plunge.seek :locked @20:56:28.96 Air.in :fast @20:56:29.54 Air.seek :locked @20:56:31.29 Carousel.reconfigure :locked @20:56:31.51 Powering cartridge bus @20:56:34.19 Gate.setIR 1750,5000,0,6 @20:56:34.32 Gate.setIR 0,5000,0,6 @20:56:34.42 PuckHeater.configure :default #ambient 24.4C @20:56:34.53 Gate.power :heat,:ON,limit to 5000mA @20:56:35.23 Mounted lysis Cartridge board#Q05048 @20:56:35.29 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:56:35.48 Toroid.in :hold @20:56:35.61 Toroid.seek :cartridge @20:56:37.53 Toroid.in :default @20:56:37.66 Toroid.seek :clear @20:56:39.52 Waiting for n to answer "record Vacuum" @20:56:52.26 Answered record Vacuum"" @20:56:52.42 Toroid.in :hold @20:56:52.53 Toroid.seek :bypass @20:56:54.21 Toroid.in :default @20:56:54.32 Toroid.seek :clear @20:56:55.98 Waiting for n to answer "recharge if needed" @20:56:57.11 Answered recharge if needed"" @20:56:57.12 Vacuuming cartridge 58 @20:56:57.38 Gate.power :heat,:OFF @20:56:57.43 Gate.power :cartridge,:OFF @20:56:57.55 Carousel.in :default @20:56:57.76 Carousel.home.seek 57 @20:56:58.24 Air.in :unlocking @20:56:58.44 Air.seek :clear @20:56:59.92 Lysis1.in :unlocking @20:57:00.13 Lysis1.seek :clear @20:57:00.21 Plunge.in :unlocking @20:57:00.39 Plunge.seek :clear @20:57:01.67 Carousel.seek 58 @20:57:02.27 Lysis1.in :fast @20:57:02.38 Lysis1.seek :locked @20:57:05.06 Plunge.in :default @20:57:05.18 Plunge.seek :locked @20:57:05.39 Air.in :fast @20:57:05.50 Air.seek :locked @20:57:07.12 Carousel.reconfigure :locked @20:57:07.34 Powering cartridge bus @20:57:10.04 Gate.setIR 1750,5000,0,6 @20:57:10.17 Gate.setIR 0,5000,0,6 @20:57:10.28 Gate.power :cartridge,:OFF @20:57:10.86 Mounted archive Cartridge @20:57:11.66 Toroid.in :hold @20:57:11.77 Toroid.seek :cartridge @20:57:13.68 Toroid.in :default @20:57:13.79 Toroid.seek :clear @20:57:15.69 Waiting for n to answer "record Vacuum" @20:57:20.12 Answered record Vacuum"" @20:57:20.27 Toroid.in :hold @20:57:20.38 Toroid.seek :bypass @20:57:22.06 Toroid.in :default @20:57:22.22 Toroid.seek :clear @20:57:23.89 Waiting for n to answer "recharge if needed" @20:57:25.05 Answered recharge if needed"" @20:57:25.06 Vacuuming cartridge 59 @20:57:25.39 Carousel.in :default @20:57:25.56 Carousel.home.seek 58 @20:57:26.09 Air.in :unlocking @20:57:26.30 Air.seek :clear @20:57:27.77 Lysis1.in :unlocking @20:57:27.98 Lysis1.seek :clear @20:57:28.06 Plunge.in :unlocking @20:57:28.23 Plunge.seek :clear @20:57:29.46 Carousel.seek 59 @20:57:30.05 Lysis1.in :fast @20:57:30.16 Lysis1.seek :locked @20:57:32.85 Plunge.in :default @20:57:33.00 Plunge.seek :locked @20:57:33.17 Air.in :fast @20:57:33.27 Air.seek :locked @20:57:34.89 Carousel.reconfigure :locked @20:57:35.10 Powering cartridge bus @20:57:37.78 Gate.setIR 1750,5000,0,6 @20:57:37.91 Gate.setIR 0,5000,0,6 @20:57:38.01 PuckHeater.configure :default #ambient 24.4C @20:57:38.12 Gate.power :heat,:ON,limit to 5000mA @20:57:38.85 Mounted lysis Cartridge board#Q05037 @20:57:38.91 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:57:39.10 Toroid.in :hold @20:57:39.22 Toroid.seek :cartridge @20:57:41.09 Toroid.in :default @20:57:41.20 Toroid.seek :clear @20:57:43.06 Waiting for n to answer "record Vacuum" @20:58:02.92 Answered record Vacuum"" @20:58:03.07 Toroid.in :hold @20:58:03.18 Toroid.seek :bypass @20:58:05.52 Toroid.in :default @20:58:05.65 Toroid.seek :clear @20:58:07.34 Waiting for n to answer "recharge if needed" @20:58:08.47 Answered recharge if needed"" @20:58:08.49 Vacuuming cartridge 60 @20:58:08.76 Gate.power :heat,:OFF @20:58:08.79 Gate.power :cartridge,:OFF @20:58:08.96 Carousel.in :default @20:58:09.15 Carousel.home.seek 59 @20:58:09.72 Air.in :unlocking @20:58:09.92 Air.seek :clear @20:58:11.39 Lysis1.in :unlocking @20:58:11.61 Lysis1.seek :clear @20:58:11.70 Plunge.in :unlocking @20:58:11.89 Plunge.seek :clear @20:58:13.12 Carousel.seek 60 @20:58:13.74 Lysis1.in :fast @20:58:13.85 Lysis1.seek :locked @20:58:16.52 Plunge.in :default @20:58:16.67 Plunge.seek :locked @20:58:16.83 Air.in :fast @20:58:16.94 Air.seek :locked @20:58:18.56 Carousel.reconfigure :locked @20:58:18.80 Powering cartridge bus @20:58:21.51 Gate.setIR 1750,5000,0,6 @20:58:21.66 Gate.setIR 0,5000,0,6 @20:58:21.76 PuckHeater.configure :default #ambient 24.4C @20:58:21.89 Gate.power :heat,:ON,limit to 5000mA @20:58:22.58 Mounted lysis Cartridge board#Q05054 @20:58:22.65 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:58:22.82 Toroid.in :hold @20:58:22.92 Toroid.seek :cartridge @20:58:24.80 Toroid.in :default @20:58:24.91 Toroid.seek :clear @20:58:26.77 Waiting for n to answer "record Vacuum" @20:58:32.72 Answered record Vacuum"" @20:58:32.88 Toroid.in :hold @20:58:32.98 Toroid.seek :bypass @20:58:34.66 Toroid.in :default @20:58:34.78 Toroid.seek :clear @20:58:36.44 Waiting for n to answer "recharge if needed" @20:58:38.61 Answered recharge if needed"" @20:58:38.67 Gate.power :heat,:OFF @20:58:38.69 Gate.power :cartridge,:OFF @20:58:38.83 Carousel.in :default @20:58:39.00 Carousel.home.seek 60 @20:58:40.17 Air.in :unlocking @20:58:40.35 Air.seek :clear @20:58:41.78 Lysis1.in :unlocking @20:58:42.00 Lysis1.seek :clear @20:58:42.08 Plunge.in :unlocking @20:58:42.27 Plunge.seek :clear @20:58:43.48 Waiting for n to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @20:58:51.66 UserAbort in n -- User Abort!! @20:59:31.49 -> recVac 1..49 @20:59:31.52 Vacuuming cartridge 1 @20:59:31.92 Carousel.seek 1 @20:59:44.30 Lysis1.in :fast @20:59:44.42 Lysis1.seek :locked @20:59:47.09 Plunge.in :default @20:59:47.23 Plunge.seek :locked @20:59:47.39 Air.in :fast @20:59:47.50 Air.seek :locked @20:59:49.11 Carousel.reconfigure :locked @20:59:49.33 Powering cartridge bus @20:59:52.03 Gate.setIR 1750,5000,0,6 @20:59:52.19 Gate.setIR 0,5000,0,6 @20:59:52.30 PuckHeater.configure :default #ambient 24.4C @20:59:52.42 Gate.power :heat,:ON,limit to 5000mA @20:59:53.16 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @20:59:53.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:59:53.42 Toroid.in :hold @20:59:53.54 Toroid.seek :cartridge @20:59:55.43 Toroid.in :default @20:59:55.54 Toroid.seek :clear @20:59:57.39 Waiting for n to answer "record Vacuum" @21:00:02.32 Answered record Vacuum"" @21:00:02.47 Toroid.in :hold @21:00:02.58 Toroid.seek :bypass @21:00:04.26 Toroid.in :default @21:00:04.38 Toroid.seek :clear @21:00:06.04 Waiting for n to answer "recharge if needed" @21:00:09.61 Answered recharge if needed"" @21:00:09.63 Vacuuming cartridge 2 @21:00:09.91 Gate.power :heat,:OFF @21:00:09.94 Gate.power :cartridge,:OFF @21:00:10.08 Carousel.in :default @21:00:10.26 Carousel.home.seek 1 @21:00:10.74 Air.in :unlocking @21:00:10.95 Air.seek :clear @21:00:12.39 Lysis1.in :unlocking @21:00:12.57 Lysis1.seek :clear @21:00:12.72 Plunge.in :unlocking @21:00:12.85 Plunge.seek :clear @21:00:13.99 Carousel.seek 2 @21:00:14.59 Lysis1.in :fast @21:00:14.71 Lysis1.seek :locked @21:00:17.38 Plunge.in :default @21:00:17.52 Plunge.seek :locked @21:00:17.73 Air.in :fast @21:00:17.83 Air.seek :locked @21:00:19.45 Carousel.reconfigure :locked @21:00:19.70 Powering cartridge bus @21:00:22.37 Gate.setIR 1750,5000,0,6 @21:00:22.51 Gate.setIR 0,5000,0,6 @21:00:22.61 PuckHeater.configure :default #ambient 24.4C @21:00:22.73 Gate.power :heat,:ON,limit to 5000mA @21:00:23.43 Mounted lysis Cartridge board#Q05087 @21:00:23.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:00:23.71 Toroid.in :hold @21:00:23.83 Toroid.seek :cartridge @21:00:25.71 Toroid.in :default @21:00:25.82 Toroid.seek :clear @21:00:27.69 Waiting for n to answer "record Vacuum" @21:00:32.32 Answered record Vacuum"" @21:00:32.47 Toroid.in :hold @21:00:32.58 Toroid.seek :bypass @21:00:34.26 Toroid.in :default @21:00:34.37 Toroid.seek :clear @21:00:36.04 Waiting for n to answer "recharge if needed" @21:00:39.69 Answered recharge if needed"" @21:00:39.71 Vacuuming cartridge 3 @21:00:39.96 Gate.power :heat,:OFF @21:00:39.99 Gate.power :cartridge,:OFF @21:00:40.13 Carousel.in :default @21:00:40.30 Carousel.home.seek 2 @21:00:40.79 Air.in :unlocking @21:00:40.96 Air.seek :clear @21:00:42.35 Lysis1.in :unlocking @21:00:42.62 Lysis1.seek :clear @21:00:42.66 Plunge.in :unlocking @21:00:42.84 Plunge.seek :clear @21:00:44.08 Carousel.seek 3 @21:00:45.26 Lysis1.in :fast @21:00:45.37 Lysis1.seek :locked @21:00:48.04 Plunge.in :default @21:00:48.19 Plunge.seek :locked @21:00:48.36 Air.in :fast @21:00:48.46 Air.seek :locked @21:00:50.06 Carousel.reconfigure :locked @21:00:50.30 Powering cartridge bus @21:00:52.96 Gate.setIR 1750,5000,0,6 @21:00:53.09 Gate.setIR 0,5000,0,6 @21:00:53.22 Gate.power :cartridge,:OFF @21:00:53.80 Mounted archive Cartridge @21:00:53.99 Toroid.in :hold @21:00:54.10 Toroid.seek :cartridge @21:00:55.98 Toroid.in :default @21:00:56.09 Toroid.seek :clear @21:00:57.95 Waiting for n to answer "record Vacuum" @21:01:03.35 Answered record Vacuum"" @21:01:03.51 Toroid.in :hold @21:01:03.61 Toroid.seek :bypass @21:01:05.32 Toroid.in :default @21:01:05.43 Toroid.seek :clear @21:01:07.08 Waiting for n to answer "recharge if needed" @21:01:10.93 Answered recharge if needed"" @21:01:10.95 Vacuuming cartridge 4 @21:01:11.26 Carousel.in :default @21:01:11.45 Carousel.home.seek 3 @21:01:11.95 Air.in :unlocking @21:01:12.14 Air.seek :clear @21:01:13.61 Lysis1.in :unlocking @21:01:13.83 Lysis1.seek :clear @21:01:13.99 Plunge.in :unlocking @21:01:14.10 Plunge.seek :clear @21:01:15.24 Carousel.seek 4 @21:01:15.85 Lysis1.in :fast @21:01:15.96 Lysis1.seek :locked @21:01:18.63 Plunge.in :default @21:01:18.78 Plunge.seek :locked @21:01:18.95 Air.in :fast @21:01:19.05 Air.seek :locked @21:01:20.67 Carousel.reconfigure :locked @21:01:20.88 Powering cartridge bus @21:01:23.54 Gate.setIR 1750,5000,0,6 @21:01:24.30 Gate.setIR 0,5000,0,6 @21:01:24.40 PuckHeater.configure :default #ambient 24.4C @21:01:24.50 Gate.power :heat,:ON,limit to 5000mA @21:01:25.20 Mounted lysis Cartridge board#Q05051 @21:01:25.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:01:25.45 Toroid.in :hold @21:01:25.57 Toroid.seek :cartridge @21:01:27.50 Toroid.in :default @21:01:27.63 Toroid.seek :clear @21:01:29.51 Waiting for n to answer "record Vacuum" @21:01:35.91 Answered record Vacuum"" @21:01:36.07 Toroid.in :hold @21:01:36.18 Toroid.seek :bypass @21:01:37.86 Toroid.in :default @21:01:37.97 Toroid.seek :clear @21:01:39.64 Waiting for n to answer "recharge if needed" @21:01:43.57 Answered recharge if needed"" @21:01:43.59 Vacuuming cartridge 5 @21:01:43.86 Gate.power :heat,:OFF @21:01:43.90 Gate.power :cartridge,:OFF @21:01:44.03 Carousel.in :default @21:01:44.20 Carousel.home.seek 4 @21:01:44.67 Air.in :unlocking @21:01:44.85 Air.seek :clear @21:01:46.24 Lysis1.in :unlocking @21:01:46.47 Lysis1.seek :clear @21:01:46.57 Plunge.in :unlocking @21:01:46.72 Plunge.seek :clear @21:01:47.90 Carousel.seek 5 @21:01:48.49 Lysis1.in :fast @21:01:48.61 Lysis1.seek :locked @21:01:51.28 Plunge.in :default @21:01:51.43 Plunge.seek :locked @21:01:51.60 Air.in :fast @21:01:51.74 Air.seek :locked @21:01:53.36 Carousel.reconfigure :locked @21:01:53.58 Powering cartridge bus @21:01:56.25 Gate.setIR 1750,5000,0,6 @21:01:56.39 Gate.setIR 0,5000,0,6 @21:01:56.50 Gate.power :cartridge,:OFF @21:01:57.10 Mounted archive Cartridge @21:01:57.28 Toroid.in :hold @21:01:57.40 Toroid.seek :cartridge @21:01:59.27 Toroid.in :default @21:01:59.39 Toroid.seek :clear @21:02:01.24 Waiting for n to answer "record Vacuum" @21:02:05.31 Answered record Vacuum"" @21:02:05.46 Toroid.in :hold @21:02:06.19 Toroid.seek :bypass @21:02:07.87 Toroid.in :default @21:02:07.98 Toroid.seek :clear @21:02:09.67 Waiting for n to answer "recharge if needed" @21:02:11.60 Answered recharge if needed"" @21:02:11.63 Vacuuming cartridge 6 @21:02:11.98 Carousel.in :default @21:02:12.16 Carousel.home.seek 5 @21:02:12.65 Air.in :unlocking @21:02:12.84 Air.seek :clear @21:02:14.25 Lysis1.in :unlocking @21:02:14.45 Lysis1.seek :clear @21:02:14.57 Plunge.in :unlocking @21:02:14.71 Plunge.seek :clear @21:02:15.91 Carousel.seek 6 @21:02:16.49 Lysis1.in :fast @21:02:16.60 Lysis1.seek :locked @21:02:19.28 Plunge.in :default @21:02:19.43 Plunge.seek :locked @21:02:19.59 Air.in :fast @21:02:19.72 Air.seek :locked @21:02:21.33 Carousel.reconfigure :locked @21:02:21.55 Powering cartridge bus @21:02:24.22 Gate.setIR 1750,5000,0,6 @21:02:24.36 Gate.setIR 0,5000,0,6 @21:02:24.46 PuckHeater.configure :default #ambient 24.4C @21:02:24.57 Gate.power :heat,:ON,limit to 5000mA @21:02:25.28 Mounted lysis Cartridge board#Q05049 @21:02:25.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:02:25.53 Toroid.in :hold @21:02:25.67 Toroid.seek :cartridge @21:02:27.56 Toroid.in :default @21:02:27.70 Toroid.seek :clear @21:02:29.55 Waiting for n to answer "record Vacuum" @21:02:33.87 Answered record Vacuum"" @21:02:34.03 Toroid.in :hold @21:02:34.13 Toroid.seek :bypass @21:02:35.82 Toroid.in :default @21:02:35.94 Toroid.seek :clear @21:02:37.60 Waiting for n to answer "recharge if needed" @21:02:42.27 Answered recharge if needed"" @21:02:42.28 Vacuuming cartridge 7 @21:02:42.54 Gate.power :heat,:OFF @21:02:42.58 Gate.power :cartridge,:OFF @21:02:42.72 Carousel.in :default @21:02:42.90 Carousel.home.seek 6 @21:02:43.97 Air.in :unlocking @21:02:44.18 Air.seek :clear @21:02:45.68 Lysis1.in :unlocking @21:02:45.91 Lysis1.seek :clear @21:02:46.00 Plunge.in :unlocking @21:02:46.17 Plunge.seek :clear @21:02:47.39 Carousel.seek 7 @21:02:47.97 Lysis1.in :fast @21:02:48.08 Lysis1.seek :locked @21:02:50.77 Plunge.in :default @21:02:50.92 Plunge.seek :locked @21:02:51.08 Air.in :fast @21:02:51.20 Air.seek :locked @21:02:52.82 Carousel.reconfigure :locked @21:02:53.04 Powering cartridge bus @21:02:55.70 Gate.setIR 1750,5000,0,6 @21:02:55.83 Gate.setIR 0,5000,0,6 @21:02:55.94 Gate.power :cartridge,:OFF @21:02:56.53 Mounted archive Cartridge @21:02:56.71 Toroid.in :hold @21:02:56.81 Toroid.seek :cartridge @21:02:58.69 Toroid.in :default @21:02:58.80 Toroid.seek :clear @21:03:00.65 Waiting for n to answer "record Vacuum" @21:03:04.49 Answered record Vacuum"" @21:03:04.64 Toroid.in :hold @21:03:04.74 Toroid.seek :bypass @21:03:06.43 Toroid.in :default @21:03:06.54 Toroid.seek :clear @21:03:08.20 Waiting for n to answer "recharge if needed" @21:03:14.52 Answered recharge if needed"" @21:03:14.54 Vacuuming cartridge 8 @21:03:14.85 Carousel.in :default @21:03:15.03 Carousel.home.seek 7 @21:03:15.50 Air.in :unlocking @21:03:15.76 Air.seek :clear @21:03:17.25 Lysis1.in :unlocking @21:03:17.45 Lysis1.seek :clear @21:03:17.53 Plunge.in :unlocking @21:03:17.75 Plunge.seek :clear @21:03:19.56 Carousel.seek 8 @21:03:20.24 Lysis1.in :fast @21:03:20.36 Lysis1.seek :locked @21:03:23.03 Plunge.in :default @21:03:23.18 Plunge.seek :locked @21:03:23.34 Air.in :fast @21:03:23.44 Air.seek :locked @21:03:25.06 Carousel.reconfigure :locked @21:03:25.28 Powering cartridge bus @21:03:27.95 Gate.setIR 1750,5000,0,6 @21:03:28.09 Gate.setIR 0,5000,0,6 @21:03:28.19 PuckHeater.configure :default #ambient 24.4C @21:03:28.29 Gate.power :heat,:ON,limit to 5000mA @21:03:29.00 Mounted lysis Cartridge board#Q05061 @21:03:29.08 Using this cartridge's heat loss curve for HeatingAccelInCarousel @21:03:29.24 Toroid.in :hold @21:03:29.34 Toroid.seek :cartridge @21:03:31.21 Toroid.in :default @21:03:31.32 Toroid.seek :clear @21:03:33.18 Waiting for n to answer "record Vacuum" @21:03:42.68 Answered record Vacuum"" @21:03:42.84 Toroid.in :hold @21:03:42.94 Toroid.seek :bypass @21:03:44.62 Toroid.in :default @21:03:44.73 Toroid.seek :clear @21:03:46.39 Waiting for n to answer "recharge if needed" @21:03:51.49 Answered recharge if needed"" @21:03:51.51 Vacuuming cartridge 9 @21:03:51.78 Gate.power :heat,:OFF @21:03:51.81 Gate.power :cartridge,:OFF @21:03:51.97 Carousel.in :default @21:03:52.14 Carousel.home.seek 8 @21:03:52.60 Air.in :unlocking @21:03:52.77 Air.seek :clear @21:03:54.16 Lysis1.in :unlocking @21:03:54.34 Lysis1.seek :clear @21:03:54.49 Plunge.in :unlocking @21:03:54.61 Plunge.seek :clear @21:03:55.75 Carousel.seek 9 @21:03:56.34 Lysis1.in :fast @21:03:56.46 Lysis1.seek :locked @21:03:59.15 Plunge.in :default @21:03:59.27 Plunge.seek :locked @21:03:59.46 Air.in :fast @21:04:00.22 Air.seek :locked @21:04:01.85 Carousel.reconfigure :locked @21:04:02.07 Powering cartridge bus @21:04:04.73 Gate.setIR 1750,5000,0,6 @21:04:04.87 Gate.setIR 0,5000,0,6 @21:04:04.98 Gate.power :cartridge,:OFF @21:04:05.57 Mounted archive Cartridge @21:04:05.80 Toroid.in :hold @21:04:05.91 Toroid.seek :cartridge @21:04:07.80 Toroid.in :default @21:04:07.91 Toroid.seek :clear @21:04:09.77 Waiting for n to answer "record Vacuum" @21:04:16.37 Answered record Vacuum"" @21:04:16.53 Toroid.in :hold @21:04:16.63 Toroid.seek :bypass @21:04:18.32 Toroid.in :default @21:04:18.43 Toroid.seek :clear @21:04:20.08 Waiting for n to answer "recharge if needed" @21:04:20.86 Answered recharge if needed"" @21:04:20.87 Vacuuming cartridge 10 @21:04:21.20 Carousel.in :default @21:04:21.39 Carousel.home.seek 9 @21:04:21.92 Air.in :unlocking @21:04:22.09 Air.seek :clear @21:04:23.49 Lysis1.in :unlocking @21:04:23.74 Lysis1.seek :clear @21:04:23.82 Plunge.in :unlocking @21:04:23.99 Plunge.seek :clear @21:04:25.22 Carousel.seek 10 @21:04:25.84 Lysis1.in :fast @21:04:25.95 Lysis1.seek :locked @21:04:28.64 Plunge.in :default @21:04:28.76 Plunge.seek :locked @21:04:28.96 Air.in :fast @21:04:29.07 Air.seek :locked @21:04:30.69 Carousel.reconfigure :locked @21:04:30.90 Powering cartridge bus @21:04:33.58 Gate.setIR 1750,5000,0,6 @21:04:33.72 Gate.setIR 0,5000,0,6 @21:04:33.82 PuckHeater.configure :default #ambient 24.4C @21:04:33.92 Gate.power :heat,:ON,limit to 5000mA @21:04:34.61 Mounted lysis Cartridge board#Q05060 @21:04:34.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:04:34.85 Toroid.in :hold @21:04:35.52 Toroid.seek :cartridge @21:04:37.48 Toroid.in :default @21:04:37.59 Toroid.seek :clear @21:04:39.48 Waiting for n to answer "record Vacuum" @21:04:43.12 Answered record Vacuum"" @21:04:43.30 Toroid.in :hold @21:04:43.41 Toroid.seek :bypass @21:04:45.09 Toroid.in :default @21:04:45.19 Toroid.seek :clear @21:04:46.86 Waiting for n to answer "recharge if needed" @21:04:50.68 Answered recharge if needed"" @21:04:50.69 Vacuuming cartridge 11 @21:04:50.94 Gate.power :heat,:OFF @21:04:50.97 Gate.power :cartridge,:OFF @21:04:51.12 Carousel.in :default @21:04:51.30 Carousel.home.seek 10 @21:04:51.83 Air.in :unlocking @21:04:52.01 Air.seek :clear @21:04:53.40 Lysis1.in :unlocking @21:04:53.61 Lysis1.seek :clear @21:04:53.70 Plunge.in :unlocking @21:04:53.89 Plunge.seek :clear @21:04:55.12 Carousel.seek 11 @21:04:55.75 Lysis1.in :fast @21:04:55.87 Lysis1.seek :locked @21:04:58.54 Plunge.in :default @21:04:58.69 Plunge.seek :locked @21:04:58.86 Air.in :fast @21:04:58.97 Air.seek :locked @21:05:00.57 Carousel.reconfigure :locked @21:05:00.81 Powering cartridge bus @21:05:03.49 Gate.setIR 1750,5000,0,6 @21:05:03.63 Gate.setIR 0,5000,0,6 @21:05:03.77 Gate.power :cartridge,:OFF @21:05:04.35 Mounted archive Cartridge @21:05:04.54 Toroid.in :hold @21:05:04.64 Toroid.seek :cartridge @21:05:06.51 Toroid.in :default @21:05:06.62 Toroid.seek :clear @21:05:08.48 Waiting for n to answer "record Vacuum" @21:05:14.33 Answered record Vacuum"" @21:05:14.49 Toroid.in :hold @21:05:14.59 Toroid.seek :bypass @21:05:16.27 Toroid.in :default @21:05:16.38 Toroid.seek :clear @21:05:18.04 Waiting for n to answer "recharge if needed" @21:05:18.83 Answered recharge if needed"" @21:05:18.84 Vacuuming cartridge 12 @21:05:19.79 Carousel.in :default @21:05:19.97 Carousel.home.seek 11 @21:05:20.46 Air.in :unlocking @21:05:20.65 Air.seek :clear @21:05:22.15 Lysis1.in :unlocking @21:05:22.35 Lysis1.seek :clear @21:05:22.43 Plunge.in :unlocking @21:05:22.60 Plunge.seek :clear @21:05:23.84 Carousel.seek 12 @21:05:24.43 Lysis1.in :fast @21:05:24.55 Lysis1.seek :locked @21:05:27.23 Plunge.in :default @21:05:27.39 Plunge.seek :locked @21:05:27.69 Air.in :fast @21:05:27.81 Air.seek :locked @21:05:29.42 Carousel.reconfigure :locked @21:05:29.68 Powering cartridge bus @21:05:32.35 Gate.setIR 1750,5000,0,6 @21:05:32.49 Gate.setIR 0,5000,0,6 @21:05:32.59 PuckHeater.configure :default #ambient 24.4C @21:05:32.70 Gate.power :heat,:ON,limit to 5000mA @21:05:33.41 Mounted lysis Cartridge board#Q05062 @21:05:33.48 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:05:33.68 Toroid.in :hold @21:05:33.79 Toroid.seek :cartridge @21:05:35.69 Toroid.in :default @21:05:35.80 Toroid.seek :clear @21:05:37.68 Waiting for n to answer "record Vacuum" @21:05:45.15 Answered record Vacuum"" @21:05:45.31 Toroid.in :hold @21:05:45.41 Toroid.seek :bypass @21:05:47.09 Toroid.in :default @21:05:47.20 Toroid.seek :clear @21:05:48.88 Waiting for n to answer "recharge if needed" @21:05:56.21 Answered recharge if needed"" @21:05:56.23 Vacuuming cartridge 13 @21:05:56.46 Gate.power :heat,:OFF @21:05:56.49 Gate.power :cartridge,:OFF @21:05:56.63 Carousel.in :default @21:05:56.80 Carousel.home.seek 12 @21:05:57.30 Air.in :unlocking @21:05:57.52 Air.seek :clear @21:05:59.61 Lysis1.in :unlocking @21:05:59.83 Lysis1.seek :clear @21:06:00.00 Plunge.in :unlocking @21:06:00.12 Plunge.seek :clear @21:06:01.27 Carousel.seek 13 @21:06:01.87 Lysis1.in :fast @21:06:01.98 Lysis1.seek :locked @21:06:04.65 Plunge.in :default @21:06:04.76 Plunge.seek :locked @21:06:04.99 Air.in :fast @21:06:05.09 Air.seek :locked @21:06:06.70 Carousel.reconfigure :locked @21:06:06.92 Powering cartridge bus @21:06:09.58 Gate.setIR 1750,5000,0,6 @21:06:09.71 Gate.setIR 0,5000,0,6 @21:06:09.82 Gate.power :cartridge,:OFF @21:06:10.40 Mounted archive Cartridge @21:06:10.60 Toroid.in :hold @21:06:10.70 Toroid.seek :cartridge @21:06:12.59 Toroid.in :default @21:06:12.70 Toroid.seek :clear @21:06:14.56 Waiting for n to answer "record Vacuum" @21:06:18.33 Answered record Vacuum"" @21:06:18.49 Toroid.in :hold @21:06:18.60 Toroid.seek :bypass @21:06:20.28 Toroid.in :default @21:06:20.39 Toroid.seek :clear @21:06:22.05 Waiting for n to answer "recharge if needed" @21:06:24.66 Answered recharge if needed"" @21:06:24.68 Vacuuming cartridge 14 @21:06:25.03 Carousel.in :default @21:06:25.22 Carousel.home.seek 13 @21:06:25.76 Air.in :unlocking @21:06:25.94 Air.seek :clear @21:06:27.34 Lysis1.in :unlocking @21:06:27.56 Lysis1.seek :clear @21:06:27.70 Plunge.in :unlocking @21:06:27.86 Plunge.seek :clear @21:06:29.09 Carousel.seek 14 @21:06:29.72 Lysis1.in :fast @21:06:29.83 Lysis1.seek :locked @21:06:32.51 Plunge.in :default @21:06:33.25 Plunge.seek :locked @21:06:33.45 Air.in :fast @21:06:33.57 Air.seek :locked @21:06:35.22 Carousel.reconfigure :locked @21:06:35.43 Powering cartridge bus @21:06:38.10 Gate.setIR 1750,5000,0,6 @21:06:38.23 Gate.setIR 0,5000,0,6 @21:06:38.34 PuckHeater.configure :default #ambient 24.4C @21:06:38.46 Gate.power :heat,:ON,limit to 5000mA @21:06:39.16 Mounted lysis Cartridge board#Q05036 @21:06:39.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:06:39.40 Toroid.in :hold @21:06:39.52 Toroid.seek :cartridge @21:06:41.39 Toroid.in :default @21:06:41.50 Toroid.seek :clear @21:06:43.36 Waiting for n to answer "record Vacuum" @21:06:48.13 Answered record Vacuum"" @21:06:48.28 Toroid.in :hold @21:06:48.38 Toroid.seek :bypass @21:06:50.06 Toroid.in :default @21:06:50.18 Toroid.seek :clear @21:06:51.84 Waiting for n to answer "recharge if needed" @21:06:55.51 Answered recharge if needed"" @21:06:55.53 Vacuuming cartridge 15 @21:06:55.81 Gate.power :heat,:OFF @21:06:55.86 Gate.power :cartridge,:OFF @21:06:55.99 Carousel.in :default @21:06:56.16 Carousel.home.seek 14 @21:06:56.63 Air.in :unlocking @21:06:56.81 Air.seek :clear @21:06:58.28 Lysis1.in :unlocking @21:06:58.51 Plunge.in :unlocking @21:06:58.54 Lysis1.seek :clear @21:06:58.69 Plunge.seek :clear @21:06:59.99 Carousel.seek 15 @21:07:00.59 Lysis1.in :fast @21:07:00.70 Lysis1.seek :locked @21:07:03.36 Plunge.in :default @21:07:03.47 Plunge.seek :locked @21:07:03.73 Air.in :fast @21:07:03.84 Air.seek :locked @21:07:05.45 Carousel.reconfigure :locked @21:07:05.71 Powering cartridge bus @21:07:08.38 Gate.setIR 1750,5000,0,6 @21:07:08.51 Gate.setIR 0,5000,0,6 @21:07:09.23 Gate.power :cartridge,:OFF @21:07:09.81 Mounted archive Cartridge @21:07:10.00 Toroid.in :hold @21:07:10.12 Toroid.seek :cartridge @21:07:12.01 Toroid.in :default @21:07:12.12 Toroid.seek :clear @21:07:13.98 Waiting for n to answer "record Vacuum" @21:07:18.34 Answered record Vacuum"" @21:07:18.49 Toroid.in :hold @21:07:18.59 Toroid.seek :bypass @21:07:20.26 Toroid.in :default @21:07:20.36 Toroid.seek :clear @21:07:22.03 Waiting for n to answer "recharge if needed" @21:07:29.24 Answered recharge if needed"" @21:07:29.26 Vacuuming cartridge 16 @21:07:29.58 Carousel.in :default @21:07:29.86 Carousel.home.seek 15 @21:07:30.35 Air.in :unlocking @21:07:30.53 Air.seek :clear @21:07:31.94 Lysis1.in :unlocking @21:07:32.15 Lysis1.seek :clear @21:07:32.22 Plunge.in :unlocking @21:07:32.40 Plunge.seek :clear @21:07:33.63 Carousel.seek 16 @21:07:34.25 Lysis1.in :fast @21:07:34.37 Lysis1.seek :locked @21:07:37.04 Plunge.in :default @21:07:37.22 Plunge.seek :locked @21:07:37.38 Air.in :fast @21:07:37.49 Air.seek :locked @21:07:39.10 Carousel.reconfigure :locked @21:07:39.32 Powering cartridge bus @21:07:41.99 Gate.setIR 1750,5000,0,6 @21:07:42.12 Gate.setIR 0,5000,0,6 @21:07:42.23 PuckHeater.configure :default #ambient 24.4C @21:07:42.34 Gate.power :heat,:ON,limit to 5000mA @21:07:45.03 Mounted lysis Cartridge board#Q05080 @21:07:45.08 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:07:45.27 Toroid.in :hold @21:07:45.38 Toroid.seek :cartridge @21:07:47.24 Toroid.in :default @21:07:47.35 Toroid.seek :clear @21:07:49.21 Waiting for n to answer "record Vacuum" @21:07:54.79 Answered record Vacuum"" @21:07:54.96 Toroid.in :hold @21:07:55.06 Toroid.seek :bypass @21:07:57.39 Toroid.in :default @21:07:57.50 Toroid.seek :clear @21:07:59.16 Waiting for n to answer "recharge if needed" @21:08:02.68 Answered recharge if needed"" @21:08:02.70 Vacuuming cartridge 17 @21:08:02.96 Gate.power :heat,:OFF @21:08:02.99 Gate.power :cartridge,:OFF @21:08:03.13 Carousel.in :default @21:08:03.31 Carousel.home.seek 16 @21:08:03.83 Air.in :unlocking @21:08:04.02 Air.seek :clear @21:08:05.40 Lysis1.in :unlocking @21:08:05.59 Lysis1.seek :clear @21:08:05.79 Plunge.in :unlocking @21:08:05.92 Plunge.seek :clear @21:08:07.07 Carousel.seek 17 @21:08:07.69 Lysis1.in :fast @21:08:07.81 Lysis1.seek :locked @21:08:10.47 Plunge.in :default @21:08:10.59 Plunge.seek :locked @21:08:10.79 Air.in :fast @21:08:10.90 Air.seek :locked @21:08:12.52 Carousel.reconfigure :locked @21:08:12.74 Powering cartridge bus @21:08:15.42 Gate.setIR 1750,5000,0,6 @21:08:15.56 Gate.setIR 0,5000,0,6 @21:08:15.72 Gate.power :cartridge,:OFF @21:08:16.31 Mounted archive Cartridge @21:08:16.50 Toroid.in :hold @21:08:16.61 Toroid.seek :cartridge @21:08:18.49 Toroid.in :default @21:08:18.60 Toroid.seek :clear @21:08:20.45 Waiting for n to answer "record Vacuum" @21:08:24.28 Answered record Vacuum"" @21:08:24.45 Toroid.in :hold @21:08:24.55 Toroid.seek :bypass @21:08:26.23 Toroid.in :default @21:08:26.34 Toroid.seek :clear @21:08:28.01 Waiting for n to answer "recharge if needed" @21:08:32.68 Answered recharge if needed"" @21:08:32.70 Vacuuming cartridge 18 @21:08:33.01 Carousel.in :default @21:08:33.20 Carousel.home.seek 17 @21:08:33.71 Air.in :unlocking @21:08:34.48 Air.seek :clear @21:08:35.89 Lysis1.in :unlocking @21:08:36.11 Lysis1.seek :clear @21:08:36.18 Plunge.in :unlocking @21:08:36.35 Plunge.seek :clear @21:08:37.58 Carousel.seek 18 @21:08:38.23 Lysis1.in :fast @21:08:38.34 Lysis1.seek :locked @21:08:41.02 Plunge.in :default @21:08:41.14 Plunge.seek :locked @21:08:41.35 Air.in :fast @21:08:41.46 Air.seek :locked @21:08:43.09 Carousel.reconfigure :locked @21:08:43.39 Powering cartridge bus @21:08:46.06 Gate.setIR 1750,5000,0,6 @21:08:46.20 Gate.setIR 0,5000,0,6 @21:08:46.31 PuckHeater.configure :default #ambient 24.4C @21:08:46.42 Gate.power :heat,:ON,limit to 5000mA @21:08:47.11 Mounted lysis Cartridge board#Q05092 @21:08:47.17 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:08:47.35 Toroid.in :hold @21:08:47.46 Toroid.seek :cartridge @21:08:49.32 Toroid.in :default @21:08:49.43 Toroid.seek :clear @21:08:51.29 Waiting for n to answer "record Vacuum" @21:09:01.45 Answered record Vacuum"" @21:09:01.61 Toroid.in :hold @21:09:01.76 Toroid.seek :bypass @21:09:03.44 Toroid.in :default @21:09:03.55 Toroid.seek :clear @21:09:05.22 Waiting for n to answer "recharge if needed" @21:09:14.25 Answered recharge if needed"" @21:09:14.27 Vacuuming cartridge 19 @21:09:14.51 Gate.power :heat,:OFF @21:09:14.55 Gate.power :cartridge,:OFF @21:09:14.69 Carousel.in :default @21:09:14.88 Carousel.home.seek 18 @21:09:15.35 Air.in :unlocking @21:09:15.52 Air.seek :clear @21:09:16.88 Lysis1.in :unlocking @21:09:17.07 Lysis1.seek :clear @21:09:17.24 Plunge.in :unlocking @21:09:17.35 Plunge.seek :clear @21:09:18.49 Carousel.seek 19 @21:09:19.71 Lysis1.in :fast @21:09:19.82 Lysis1.seek :locked @21:09:22.49 Plunge.in :default @21:09:22.60 Plunge.seek :locked @21:09:22.84 Air.in :fast @21:09:22.94 Air.seek :locked @21:09:24.57 Carousel.reconfigure :locked @21:09:24.78 Powering cartridge bus @21:09:27.45 Gate.setIR 1750,5000,0,6 @21:09:27.58 Gate.setIR 0,5000,0,6 @21:09:27.73 Gate.power :cartridge,:OFF @21:09:28.31 Mounted archive Cartridge @21:09:28.50 Toroid.in :hold @21:09:28.60 Toroid.seek :cartridge @21:09:30.49 Toroid.in :default @21:09:30.60 Toroid.seek :clear @21:09:32.46 Waiting for n to answer "record Vacuum" @21:09:36.06 Answered record Vacuum"" @21:09:36.25 Toroid.in :hold @21:09:36.35 Toroid.seek :bypass @21:09:38.03 Toroid.in :default @21:09:38.15 Toroid.seek :clear @21:09:39.81 Waiting for n to answer "recharge if needed" @21:09:40.66 Answered recharge if needed"" @21:09:40.68 Vacuuming cartridge 20 @21:09:41.01 Carousel.in :default @21:09:41.20 Carousel.home.seek 19 @21:09:41.74 Air.in :unlocking @21:09:41.95 Air.seek :clear @21:09:43.43 Lysis1.in :unlocking @21:09:43.67 Lysis1.seek :clear @21:09:43.74 Plunge.in :unlocking @21:09:43.92 Plunge.seek :clear @21:09:45.14 Carousel.seek 20 @21:09:45.78 Lysis1.in :fast @21:09:45.89 Lysis1.seek :locked @21:09:48.57 Plunge.in :default @21:09:48.69 Plunge.seek :locked @21:09:48.90 Air.in :fast @21:09:49.00 Air.seek :locked @21:09:50.61 Carousel.reconfigure :locked @21:09:51.40 Powering cartridge bus @21:09:54.10 Gate.setIR 1750,5000,0,6 @21:09:54.24 Gate.setIR 0,5000,0,6 @21:09:54.34 PuckHeater.configure :default #ambient 24.4C @21:09:54.46 Gate.power :heat,:ON,limit to 5000mA @21:09:55.16 Mounted lysis Cartridge board#Q05065 @21:09:55.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:09:55.41 Toroid.in :hold @21:09:55.52 Toroid.seek :cartridge @21:09:57.40 Toroid.in :default @21:09:57.52 Toroid.seek :clear @21:09:59.38 Waiting for n to answer "record Vacuum" @21:10:02.89 Answered record Vacuum"" @21:10:03.06 Toroid.in :hold @21:10:03.16 Toroid.seek :bypass @21:10:04.84 Toroid.in :default @21:10:04.95 Toroid.seek :clear @21:10:06.61 Waiting for n to answer "recharge if needed" @21:10:10.06 Answered recharge if needed"" @21:10:10.09 Vacuuming cartridge 21 @21:10:10.33 Gate.power :heat,:OFF @21:10:10.37 Gate.power :cartridge,:OFF @21:10:10.52 Carousel.in :default @21:10:10.69 Carousel.home.seek 20 @21:10:11.17 Air.in :unlocking @21:10:11.38 Air.seek :clear @21:10:12.82 Lysis1.in :unlocking @21:10:13.03 Lysis1.seek :clear @21:10:13.09 Plunge.in :unlocking @21:10:13.29 Plunge.seek :clear @21:10:14.51 Carousel.seek 21 @21:10:15.12 Lysis1.in :fast @21:10:15.24 Lysis1.seek :locked @21:10:17.91 Plunge.in :default @21:10:18.06 Plunge.seek :locked @21:10:18.21 Air.in :fast @21:10:18.33 Air.seek :locked @21:10:19.93 Carousel.reconfigure :locked @21:10:20.16 Powering cartridge bus @21:10:22.82 Gate.setIR 1750,5000,0,6 @21:10:22.95 Gate.setIR 0,5000,0,6 @21:10:23.07 Gate.power :cartridge,:OFF @21:10:23.67 Mounted archive Cartridge @21:10:23.85 Toroid.in :hold @21:10:23.97 Toroid.seek :cartridge @21:10:25.85 Toroid.in :default @21:10:25.95 Toroid.seek :clear @21:10:28.42 Waiting for n to answer "record Vacuum" @21:10:32.08 Answered record Vacuum"" @21:10:32.24 Toroid.in :hold @21:10:32.34 Toroid.seek :bypass @21:10:34.03 Toroid.in :default @21:10:34.14 Toroid.seek :clear @21:10:35.80 Waiting for n to answer "recharge if needed" @21:10:36.62 Answered recharge if needed"" @21:10:36.64 Vacuuming cartridge 22 @21:10:36.96 Carousel.in :default @21:10:37.15 Carousel.home.seek 21 @21:10:37.66 Air.in :unlocking @21:10:37.84 Air.seek :clear @21:10:39.27 Lysis1.in :unlocking @21:10:39.51 Plunge.in :unlocking @21:10:39.53 Lysis1.seek :clear @21:10:39.78 Plunge.seek :clear @21:10:41.04 Carousel.seek 22 @21:10:41.65 Lysis1.in :fast @21:10:41.77 Lysis1.seek :locked @21:10:44.45 Plunge.in :default @21:10:44.57 Plunge.seek :locked @21:10:44.77 Air.in :fast @21:10:44.87 Air.seek :locked @21:10:46.49 Carousel.reconfigure :locked @21:10:46.70 Powering cartridge bus @21:10:49.38 Gate.setIR 1750,5000,0,6 @21:10:49.51 Gate.setIR 0,5000,0,6 @21:10:49.64 PuckHeater.configure :default #ambient 24.4C @21:10:49.77 Gate.power :heat,:ON,limit to 5000mA @21:10:50.48 Mounted lysis Cartridge board#Q05090 @21:10:50.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:10:50.72 Toroid.in :hold @21:10:50.83 Toroid.seek :cartridge @21:10:52.69 Toroid.in :default @21:10:52.79 Toroid.seek :clear @21:10:54.65 Waiting for n to answer "record Vacuum" @21:10:58.50 Answered record Vacuum"" @21:10:58.65 Toroid.in :hold @21:10:58.75 Toroid.seek :bypass @21:11:00.44 Toroid.in :default @21:11:00.54 Toroid.seek :clear @21:11:02.21 Waiting for n to answer "recharge if needed" @21:11:05.54 Answered recharge if needed"" @21:11:05.57 Vacuuming cartridge 23 @21:11:05.84 Gate.power :heat,:OFF @21:11:05.90 Gate.power :cartridge,:OFF @21:11:06.64 Carousel.in :default @21:11:06.82 Carousel.home.seek 22 @21:11:07.33 Air.in :unlocking @21:11:07.50 Air.seek :clear @21:11:08.89 Lysis1.in :unlocking @21:11:09.13 Plunge.in :unlocking @21:11:09.18 Lysis1.seek :clear @21:11:09.38 Plunge.seek :clear @21:11:10.63 Carousel.seek 23 @21:11:11.23 Lysis1.in :fast @21:11:11.34 Lysis1.seek :locked @21:11:14.01 Plunge.in :default @21:11:14.19 Plunge.seek :locked @21:11:14.33 Air.in :fast @21:11:14.44 Air.seek :locked @21:11:16.06 Carousel.reconfigure :locked @21:11:16.28 Powering cartridge bus @21:11:18.94 Gate.setIR 1750,5000,0,6 @21:11:19.07 Gate.setIR 0,5000,0,6 @21:11:19.19 Gate.power :cartridge,:OFF @21:11:19.78 Mounted archive Cartridge @21:11:19.96 Toroid.in :hold @21:11:20.08 Toroid.seek :cartridge @21:11:21.96 Toroid.in :default @21:11:22.07 Toroid.seek :clear @21:11:23.93 Waiting for n to answer "record Vacuum" @21:11:27.58 Answered record Vacuum"" @21:11:27.77 Toroid.in :hold @21:11:27.89 Toroid.seek :bypass @21:11:29.56 Toroid.in :default @21:11:29.72 Toroid.seek :clear @21:11:31.41 Waiting for n to answer "recharge if needed" @21:11:34.64 Answered recharge if needed"" @21:11:34.66 Vacuuming cartridge 24 @21:11:34.99 Carousel.in :default @21:11:35.17 Carousel.home.seek 23 @21:11:35.70 Air.in :unlocking @21:11:35.90 Air.seek :clear @21:11:37.38 Lysis1.in :unlocking @21:11:37.58 Lysis1.seek :clear @21:11:38.34 Plunge.in :unlocking @21:11:38.49 Plunge.seek :clear @21:11:39.72 Carousel.seek 24 @21:11:40.31 Lysis1.in :fast @21:11:40.42 Lysis1.seek :locked @21:11:43.10 Plunge.in :default @21:11:43.25 Plunge.seek :locked @21:11:43.42 Air.in :fast @21:11:43.52 Air.seek :locked @21:11:45.15 Carousel.reconfigure :locked @21:11:45.38 Powering cartridge bus @21:11:48.05 Gate.setIR 1750,5000,0,6 @21:11:48.18 Gate.setIR 0,5000,0,6 @21:11:48.29 PuckHeater.configure :default #ambient 24.4C @21:11:48.40 Gate.power :heat,:ON,limit to 5000mA @21:11:49.10 Mounted lysis Cartridge board#Q05042 @21:11:49.17 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:11:49.35 Toroid.in :hold @21:11:49.46 Toroid.seek :cartridge @21:11:51.36 Toroid.in :default @21:11:51.47 Toroid.seek :clear @21:11:53.33 Waiting for n to answer "record Vacuum" @21:11:57.48 Answered record Vacuum"" @21:11:57.66 Toroid.in :hold @21:11:57.78 Toroid.seek :bypass @21:11:59.46 Toroid.in :default @21:11:59.58 Toroid.seek :clear @21:12:01.27 Waiting for n to answer "recharge if needed" @21:12:05.16 Answered recharge if needed"" @21:12:05.19 Vacuuming cartridge 25 @21:12:05.45 Gate.power :heat,:OFF @21:12:05.49 Gate.power :cartridge,:OFF @21:12:05.67 Carousel.in :default @21:12:05.85 Carousel.home.seek 24 @21:12:06.32 Air.in :unlocking @21:12:06.49 Air.seek :clear @21:12:07.89 Lysis1.in :unlocking @21:12:08.11 Lysis1.seek :clear @21:12:08.24 Plunge.in :unlocking @21:12:08.39 Plunge.seek :clear @21:12:09.56 Carousel.seek 25 @21:12:10.18 Lysis1.in :fast @21:12:10.30 Lysis1.seek :locked @21:12:12.96 Plunge.in :default @21:12:13.78 Plunge.seek :locked @21:12:13.94 Air.in :fast @21:12:14.04 Air.seek :locked @21:12:15.69 Carousel.reconfigure :locked @21:12:15.92 Powering cartridge bus @21:12:18.59 Gate.setIR 1750,5000,0,6 @21:12:18.73 Gate.setIR 0,5000,0,6 @21:12:18.84 Gate.power :cartridge,:OFF @21:12:19.43 Mounted archive Cartridge @21:12:19.61 Toroid.in :hold @21:12:19.76 Toroid.seek :cartridge @21:12:21.67 Toroid.in :default @21:12:21.78 Toroid.seek :clear @21:12:23.67 Waiting for n to answer "record Vacuum" @21:12:26.61 Answered record Vacuum"" @21:12:26.78 Toroid.in :hold @21:12:26.89 Toroid.seek :bypass @21:12:28.58 Toroid.in :default @21:12:28.68 Toroid.seek :clear @21:12:30.34 Waiting for n to answer "recharge if needed" @21:12:31.13 Answered recharge if needed"" @21:12:31.15 Vacuuming cartridge 26 @21:12:31.47 Carousel.in :default @21:12:31.69 Carousel.home.seek 25 @21:12:32.19 Air.in :unlocking @21:12:32.37 Air.seek :clear @21:12:33.79 Lysis1.in :unlocking @21:12:34.02 Lysis1.seek :clear @21:12:34.08 Plunge.in :unlocking @21:12:34.26 Plunge.seek :clear @21:12:35.48 Carousel.seek 26 @21:12:36.08 Lysis1.in :fast @21:12:36.19 Lysis1.seek :locked @21:12:38.87 Plunge.in :default @21:12:38.99 Plunge.seek :locked @21:12:39.20 Air.in :fast @21:12:39.30 Air.seek :locked @21:12:40.92 Carousel.reconfigure :locked @21:12:41.15 Powering cartridge bus @21:12:43.82 Gate.setIR 1750,5000,0,6 @21:12:43.95 Gate.setIR 0,5000,0,6 @21:12:44.05 PuckHeater.configure :default #ambient 24.4C @21:12:44.16 Gate.power :heat,:ON,limit to 5000mA @21:12:45.41 Mounted lysis Cartridge board#Q05059 @21:12:45.53 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:12:45.73 Toroid.in :hold @21:12:45.85 Toroid.seek :cartridge @21:12:47.73 Toroid.in :default @21:12:47.84 Toroid.seek :clear @21:12:49.70 Waiting for n to answer "record Vacuum" @21:12:52.93 Answered record Vacuum"" @21:12:53.10 Toroid.in :hold @21:12:53.22 Toroid.seek :bypass @21:12:54.90 Toroid.in :default @21:12:55.01 Toroid.seek :clear @21:12:56.66 Waiting for n to answer "recharge if needed" @21:13:02.09 Answered recharge if needed"" @21:13:02.11 Vacuuming cartridge 27 @21:13:02.36 Gate.power :heat,:OFF @21:13:02.39 Gate.power :cartridge,:OFF @21:13:02.54 Carousel.in :default @21:13:02.73 Carousel.home.seek 26 @21:13:03.21 Air.in :unlocking @21:13:03.39 Air.seek :clear @21:13:04.78 Lysis1.in :unlocking @21:13:04.97 Lysis1.seek :clear @21:13:05.12 Plunge.in :unlocking @21:13:05.25 Plunge.seek :clear @21:13:06.39 Carousel.seek 27 @21:13:06.98 Lysis1.in :fast @21:13:07.09 Lysis1.seek :locked @21:13:09.78 Plunge.in :default @21:13:09.96 Plunge.seek :locked @21:13:10.11 Air.in :fast @21:13:10.21 Air.seek :locked @21:13:11.83 Carousel.reconfigure :locked @21:13:12.06 Powering cartridge bus @21:13:14.72 Gate.setIR 1750,5000,0,6 @21:13:14.85 Gate.setIR 0,5000,0,6 @21:13:14.96 Gate.power :cartridge,:OFF @21:13:15.55 Mounted archive Cartridge @21:13:15.77 Toroid.in :hold @21:13:15.89 Toroid.seek :cartridge @21:13:17.77 Toroid.in :default @21:13:17.88 Toroid.seek :clear @21:13:19.74 Waiting for n to answer "record Vacuum" @21:13:22.80 Answered record Vacuum"" @21:13:22.99 Toroid.in :hold @21:13:23.10 Toroid.seek :bypass @21:13:24.81 Toroid.in :default @21:13:24.92 Toroid.seek :clear @21:13:26.58 Waiting for n to answer "recharge if needed" @21:13:33.17 Answered recharge if needed"" @21:13:33.18 Vacuuming cartridge 28 @21:13:33.58 Carousel.in :default @21:13:33.80 Carousel.home.seek 27 @21:13:34.33 Air.in :unlocking @21:13:34.51 Air.seek :clear @21:13:35.92 Lysis1.in :unlocking @21:13:36.15 Lysis1.seek :clear @21:13:36.23 Plunge.in :unlocking @21:13:36.40 Plunge.seek :clear @21:13:37.66 Carousel.seek 28 @21:13:38.27 Lysis1.in :fast @21:13:38.39 Lysis1.seek :locked @21:13:41.06 Plunge.in :default @21:13:41.24 Plunge.seek :locked @21:13:41.40 Air.in :fast @21:13:41.50 Air.seek :locked @21:13:43.13 Carousel.reconfigure :locked @21:13:43.36 Powering cartridge bus @21:13:46.04 Gate.setIR 1750,5000,0,6 @21:13:46.17 Gate.setIR 0,5000,0,6 @21:13:46.28 PuckHeater.configure :default #ambient 24.4C @21:13:46.39 Gate.power :heat,:ON,limit to 5000mA @21:13:47.10 Mounted lysis Cartridge board#Q05086 @21:13:47.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:13:47.36 Toroid.in :hold @21:13:47.47 Toroid.seek :cartridge @21:13:49.36 Toroid.in :default @21:13:49.47 Toroid.seek :clear @21:13:51.33 Waiting for n to answer "record Vacuum" @21:13:55.75 Answered record Vacuum"" @21:13:55.92 Toroid.in :hold @21:13:56.03 Toroid.seek :bypass @21:13:57.73 Toroid.in :default @21:13:57.84 Toroid.seek :clear @21:13:59.51 Waiting for n to answer "recharge if needed" @21:14:03.57 Answered recharge if needed"" @21:14:03.59 Vacuuming cartridge 29 @21:14:03.88 Gate.power :heat,:OFF @21:14:03.91 Gate.power :cartridge,:OFF @21:14:04.06 Carousel.in :default @21:14:04.23 Carousel.home.seek 28 @21:14:04.72 Air.in :unlocking @21:14:05.50 Air.seek :clear @21:14:07.03 Lysis1.in :unlocking @21:14:07.25 Lysis1.seek :clear @21:14:07.31 Plunge.in :unlocking @21:14:07.51 Plunge.seek :clear @21:14:08.73 Carousel.seek 29 @21:14:09.32 Lysis1.in :fast @21:14:09.43 Lysis1.seek :locked @21:14:12.09 Plunge.in :default @21:14:12.25 Plunge.seek :locked @21:14:12.42 Air.in :fast @21:14:12.53 Air.seek :locked @21:14:14.17 Carousel.reconfigure :locked @21:14:14.40 Powering cartridge bus @21:14:17.06 Gate.setIR 1750,5000,0,6 @21:14:17.20 Gate.setIR 0,5000,0,6 @21:14:17.32 Gate.power :cartridge,:OFF @21:14:17.90 Mounted archive Cartridge @21:14:18.11 Toroid.in :hold @21:14:18.23 Toroid.seek :cartridge @21:14:20.11 Toroid.in :default @21:14:20.23 Toroid.seek :clear @21:14:22.09 Waiting for n to answer "record Vacuum" @21:14:25.02 Answered record Vacuum"" @21:14:25.21 Toroid.in :hold @21:14:25.32 Toroid.seek :bypass @21:14:27.02 Toroid.in :default @21:14:27.14 Toroid.seek :clear @21:14:28.80 Waiting for n to answer "recharge if needed" @21:14:29.60 Answered recharge if needed"" @21:14:29.63 Vacuuming cartridge 30 @21:14:29.99 Carousel.in :default @21:14:30.18 Carousel.home.seek 29 @21:14:30.68 Air.in :unlocking @21:14:30.89 Air.seek :clear @21:14:32.38 Lysis1.in :unlocking @21:14:32.60 Lysis1.seek :clear @21:14:32.67 Plunge.in :unlocking @21:14:32.87 Plunge.seek :clear @21:14:34.11 Carousel.seek 30 @21:14:34.72 Lysis1.in :fast @21:14:34.83 Lysis1.seek :locked @21:14:38.12 Plunge.in :default @21:14:38.24 Plunge.seek :locked @21:14:38.48 Air.in :fast @21:14:38.59 Air.seek :locked @21:14:40.21 Carousel.reconfigure :locked @21:14:40.42 Powering cartridge bus @21:14:43.10 Gate.setIR 1750,5000,0,6 @21:14:43.23 Gate.setIR 0,5000,0,6 @21:14:43.34 PuckHeater.configure :default #ambient 24.4C @21:14:43.45 Gate.power :heat,:ON,limit to 5000mA @21:14:44.18 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @21:14:44.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:14:44.45 Toroid.in :hold @21:14:44.57 Toroid.seek :cartridge @21:14:46.47 Toroid.in :default @21:14:46.57 Toroid.seek :clear @21:14:48.42 Waiting for n to answer "record Vacuum" @21:14:51.33 Answered record Vacuum"" @21:14:51.50 Toroid.in :hold @21:14:51.65 Toroid.seek :bypass @21:14:53.36 Toroid.in :default @21:14:53.47 Toroid.seek :clear @21:14:55.13 Waiting for n to answer "recharge if needed" @21:15:01.39 Answered recharge if needed"" @21:15:01.41 Vacuuming cartridge 31 @21:15:01.69 Gate.power :heat,:OFF @21:15:01.72 Gate.power :cartridge,:OFF @21:15:01.88 Carousel.in :default @21:15:02.07 Carousel.home.seek 30 @21:15:02.55 Air.in :unlocking @21:15:02.72 Air.seek :clear @21:15:04.11 Lysis1.in :unlocking @21:15:04.33 Lysis1.seek :clear @21:15:04.39 Plunge.in :unlocking @21:15:04.58 Plunge.seek :clear @21:15:05.81 Carousel.seek 31 @21:15:06.41 Lysis1.in :fast @21:15:06.53 Lysis1.seek :locked @21:15:09.20 Plunge.in :default @21:15:09.32 Plunge.seek :locked @21:15:09.50 Air.in :fast @21:15:09.66 Air.seek :locked @21:15:11.27 Carousel.reconfigure :locked @21:15:11.50 Powering cartridge bus @21:15:14.21 Gate.setIR 1750,5000,0,6 @21:15:14.35 Gate.setIR 0,5000,0,6 @21:15:14.45 Gate.power :cartridge,:OFF @21:15:15.04 Mounted archive Cartridge @21:15:15.24 Toroid.in :hold @21:15:15.35 Toroid.seek :cartridge @21:15:17.24 Toroid.in :default @21:15:17.36 Toroid.seek :clear @21:15:19.21 Waiting for n to answer "record Vacuum" @21:15:22.28 Answered record Vacuum"" @21:15:22.46 Toroid.in :hold @21:15:22.56 Toroid.seek :bypass @21:15:24.26 Toroid.in :default @21:15:24.37 Toroid.seek :clear @21:15:26.04 Waiting for n to answer "recharge if needed" @21:15:27.87 Answered recharge if needed"" @21:15:27.89 Vacuuming cartridge 32 @21:15:28.21 Carousel.in :default @21:15:28.39 Carousel.home.seek 31 @21:15:28.88 Air.in :unlocking @21:15:29.10 Air.seek :clear @21:15:30.53 Lysis1.in :unlocking @21:15:30.73 Lysis1.seek :clear @21:15:30.81 Plunge.in :unlocking @21:15:30.99 Plunge.seek :clear @21:15:32.20 Carousel.seek 32 @21:15:32.80 Lysis1.in :fast @21:15:32.92 Lysis1.seek :locked @21:15:35.66 Plunge.in :default @21:15:35.82 Plunge.seek :locked @21:15:35.97 Air.in :fast @21:15:36.06 Air.seek :locked @21:15:37.69 Carousel.reconfigure :locked @21:15:37.91 Powering cartridge bus @21:15:40.59 Gate.setIR 1750,5000,0,6 @21:15:40.73 Gate.setIR 0,5000,0,6 @21:15:40.84 PuckHeater.configure :default #ambient 24.4C @21:15:40.95 Gate.power :heat,:ON,limit to 5000mA @21:15:41.70 Mounted lysis Cartridge board#Q05052 @21:15:41.77 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:15:41.95 Toroid.in :hold @21:15:42.06 Toroid.seek :cartridge @21:15:43.95 Toroid.in :default @21:15:44.05 Toroid.seek :clear @21:15:45.91 Waiting for n to answer "record Vacuum" @21:15:55.66 Answered record Vacuum"" @21:15:56.39 Toroid.in :hold @21:15:56.54 Toroid.seek :bypass @21:15:58.22 Toroid.in :default @21:15:58.33 Toroid.seek :clear @21:15:59.99 Waiting for n to answer "recharge if needed" @21:16:04.67 Answered recharge if needed"" @21:16:04.69 Vacuuming cartridge 33 @21:16:04.93 Gate.power :heat,:OFF @21:16:04.96 Gate.power :cartridge,:OFF @21:16:05.12 Carousel.in :default @21:16:05.29 Carousel.home.seek 32 @21:16:05.82 Air.in :unlocking @21:16:06.02 Air.seek :clear @21:16:07.50 Lysis1.in :unlocking @21:16:07.76 Lysis1.seek :clear @21:16:07.85 Plunge.in :unlocking @21:16:08.00 Plunge.seek :clear @21:16:09.22 Carousel.seek 33 @21:16:09.85 Lysis1.in :fast @21:16:09.96 Lysis1.seek :locked @21:16:12.63 Plunge.in :default @21:16:12.74 Plunge.seek :locked @21:16:12.95 Air.in :fast @21:16:13.06 Air.seek :locked @21:16:14.68 Carousel.reconfigure :locked @21:16:14.91 Powering cartridge bus @21:16:17.57 Gate.setIR 1750,5000,0,6 @21:16:17.70 Gate.setIR 0,5000,0,6 @21:16:17.81 Gate.power :cartridge,:OFF @21:16:18.39 Mounted archive Cartridge @21:16:18.58 Toroid.in :hold @21:16:18.69 Toroid.seek :cartridge @21:16:20.57 Toroid.in :default @21:16:20.68 Toroid.seek :clear @21:16:22.53 Waiting for n to answer "record Vacuum" @21:16:34.68 Answered record Vacuum"" @21:16:34.84 Toroid.in :hold @21:16:34.94 Toroid.seek :bypass @21:16:36.62 Toroid.in :default @21:16:36.73 Toroid.seek :clear @21:16:38.39 Waiting for n to answer "recharge if needed" @21:16:44.10 Answered recharge if needed"" @21:16:44.11 Vacuuming cartridge 34 @21:16:44.43 Carousel.in :default @21:16:44.62 Carousel.home.seek 33 @21:16:45.72 Air.in :unlocking @21:16:45.93 Air.seek :clear @21:16:47.38 Lysis1.in :unlocking @21:16:47.59 Lysis1.seek :clear @21:16:47.69 Plunge.in :unlocking @21:16:47.88 Plunge.seek :clear @21:16:49.10 Carousel.seek 34 @21:16:49.74 Lysis1.in :fast @21:16:49.85 Lysis1.seek :locked @21:16:52.52 Plunge.in :default @21:16:52.64 Plunge.seek :locked @21:16:52.84 Air.in :fast @21:16:52.96 Air.seek :locked @21:16:54.57 Carousel.reconfigure :locked @21:16:54.79 Powering cartridge bus @21:16:57.48 Gate.setIR 1750,5000,0,6 @21:16:57.63 Gate.setIR 0,5000,0,6 @21:16:57.76 PuckHeater.configure :default #ambient 24.4C @21:16:57.87 Gate.power :heat,:ON,limit to 5000mA @21:16:58.57 Mounted lysis Cartridge board#Q05066 @21:16:58.66 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:16:58.82 Toroid.in :hold @21:16:58.94 Toroid.seek :cartridge @21:17:00.82 Toroid.in :default @21:17:00.92 Toroid.seek :clear @21:17:02.78 Waiting for n to answer "record Vacuum" @21:17:06.52 Answered record Vacuum"" @21:17:06.68 Toroid.in :hold @21:17:06.78 Toroid.seek :bypass @21:17:08.46 Toroid.in :default @21:17:08.57 Toroid.seek :clear @21:17:10.24 Waiting for n to answer "recharge if needed" @21:17:13.38 Answered recharge if needed"" @21:17:13.39 Vacuuming cartridge 35 @21:17:13.70 Gate.power :heat,:OFF @21:17:13.72 Gate.power :cartridge,:OFF @21:17:13.89 Carousel.in :default @21:17:14.06 Carousel.home.seek 34 @21:17:14.54 Air.in :unlocking @21:17:14.75 Air.seek :clear @21:17:16.21 Lysis1.in :unlocking @21:17:16.43 Lysis1.seek :clear @21:17:16.51 Plunge.in :unlocking @21:17:16.69 Plunge.seek :clear @21:17:18.45 Carousel.seek 35 @21:17:19.04 Lysis1.in :fast @21:17:19.15 Lysis1.seek :locked @21:17:21.81 Plunge.in :default @21:17:21.99 Plunge.seek :locked @21:17:22.14 Air.in :fast @21:17:22.24 Air.seek :locked @21:17:23.87 Carousel.reconfigure :locked @21:17:24.10 Powering cartridge bus @21:17:26.76 Gate.setIR 1750,5000,0,6 @21:17:26.90 Gate.setIR 0,5000,0,6 @21:17:27.02 Gate.power :cartridge,:OFF @21:17:27.60 Mounted archive Cartridge @21:17:27.83 Toroid.in :hold @21:17:27.95 Toroid.seek :cartridge @21:17:29.83 Toroid.in :default @21:17:29.94 Toroid.seek :clear @21:17:31.79 Waiting for n to answer "record Vacuum" @21:17:35.46 Answered record Vacuum"" @21:17:35.72 Toroid.in :hold @21:17:35.82 Toroid.seek :bypass @21:17:37.50 Toroid.in :default @21:17:37.61 Toroid.seek :clear @21:17:39.31 Waiting for n to answer "recharge if needed" @21:17:40.18 Answered recharge if needed"" @21:17:40.19 Vacuuming cartridge 36 @21:17:40.52 Carousel.in :default @21:17:40.71 Carousel.home.seek 35 @21:17:41.19 Air.in :unlocking @21:17:41.36 Air.seek :clear @21:17:42.75 Lysis1.in :unlocking @21:17:42.97 Lysis1.seek :clear @21:17:43.05 Plunge.in :unlocking @21:17:43.22 Plunge.seek :clear @21:17:44.44 Carousel.seek 36 @21:17:45.02 Lysis1.in :fast @21:17:45.13 Lysis1.seek :locked @21:17:47.80 Plunge.in :default @21:17:47.94 Plunge.seek :locked @21:17:48.12 Air.in :fast @21:17:48.23 Air.seek :locked @21:17:49.85 Carousel.reconfigure :locked @21:17:50.67 Powering cartridge bus @21:17:53.34 Gate.setIR 1750,5000,0,6 @21:17:53.47 Gate.setIR 0,5000,0,6 @21:17:53.59 PuckHeater.configure :default #ambient 24.4C @21:17:53.73 Gate.power :heat,:ON,limit to 5000mA @21:17:54.44 Mounted lysis Cartridge board#Q05045 @21:17:54.52 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:17:54.68 Toroid.in :hold @21:17:54.80 Toroid.seek :cartridge @21:17:56.68 Toroid.in :default @21:17:56.79 Toroid.seek :clear @21:17:58.65 Waiting for n to answer "record Vacuum" @21:18:01.92 Answered record Vacuum"" @21:18:02.09 Toroid.in :hold @21:18:02.20 Toroid.seek :bypass @21:18:03.88 Toroid.in :default @21:18:03.99 Toroid.seek :clear @21:18:05.68 Waiting for n to answer "recharge if needed" @21:18:09.57 Answered recharge if needed"" @21:18:09.59 Vacuuming cartridge 37 @21:18:09.87 Gate.power :heat,:OFF @21:18:09.91 Gate.power :cartridge,:OFF @21:18:10.09 Carousel.in :default @21:18:10.26 Carousel.home.seek 36 @21:18:10.75 Air.in :unlocking @21:18:10.91 Air.seek :clear @21:18:12.30 Lysis1.in :unlocking @21:18:12.50 Lysis1.seek :clear @21:18:12.65 Plunge.in :unlocking @21:18:12.78 Plunge.seek :clear @21:18:13.93 Carousel.seek 37 @21:18:14.52 Lysis1.in :fast @21:18:14.64 Lysis1.seek :locked @21:18:17.31 Plunge.in :default @21:18:17.43 Plunge.seek :locked @21:18:17.63 Air.in :fast @21:18:17.77 Air.seek :locked @21:18:19.37 Carousel.reconfigure :locked @21:18:19.59 Powering cartridge bus @21:18:22.26 Gate.setIR 1750,5000,0,6 @21:18:22.40 Gate.setIR 0,5000,0,6 @21:18:22.52 Gate.power :cartridge,:OFF @21:18:23.10 Mounted archive Cartridge @21:18:23.29 Toroid.in :hold @21:18:23.40 Toroid.seek :cartridge @21:18:25.86 Toroid.in :default @21:18:26.01 Toroid.seek :clear @21:18:27.86 Waiting for n to answer "record Vacuum" @21:18:32.13 Answered record Vacuum"" @21:18:32.29 Toroid.in :hold @21:18:32.40 Toroid.seek :bypass @21:18:34.08 Toroid.in :default @21:18:34.19 Toroid.seek :clear @21:18:35.85 Waiting for n to answer "recharge if needed" @21:18:37.30 Answered recharge if needed"" @21:18:37.32 Vacuuming cartridge 38 @21:18:37.67 Carousel.in :default @21:18:37.86 Carousel.home.seek 37 @21:18:38.34 Air.in :unlocking @21:18:38.52 Air.seek :clear @21:18:39.94 Lysis1.in :unlocking @21:18:40.16 Lysis1.seek :clear @21:18:40.24 Plunge.in :unlocking @21:18:40.41 Plunge.seek :clear @21:18:41.65 Carousel.seek 38 @21:18:42.25 Lysis1.in :fast @21:18:42.36 Lysis1.seek :locked @21:18:45.04 Plunge.in :default @21:18:45.20 Plunge.seek :locked @21:18:45.37 Air.in :fast @21:18:45.47 Air.seek :locked @21:18:47.10 Carousel.reconfigure :locked @21:18:47.32 Powering cartridge bus @21:18:49.99 Gate.setIR 1750,5000,0,6 @21:18:50.12 Gate.setIR 0,5000,0,6 @21:18:50.23 PuckHeater.configure :default #ambient 24.4C @21:18:50.33 Gate.power :heat,:ON,limit to 5000mA @21:18:51.04 Mounted lysis Cartridge board#Q05034 @21:18:51.12 Using this cartridge's heat loss curve for HeatingAccelInCarousel @21:18:51.28 Toroid.in :hold @21:18:51.39 Toroid.seek :cartridge @21:18:53.26 Toroid.in :default @21:18:53.37 Toroid.seek :clear @21:18:55.22 Waiting for n to answer "record Vacuum" @21:18:58.34 Answered record Vacuum"" @21:18:58.51 Toroid.in :hold @21:18:58.61 Toroid.seek :bypass @21:19:00.29 Toroid.in :default @21:19:00.41 Toroid.seek :clear @21:19:02.07 Waiting for n to answer "recharge if needed" @21:19:06.54 Answered recharge if needed"" @21:19:06.55 Vacuuming cartridge 39 @21:19:07.41 Gate.power :heat,:OFF @21:19:07.43 Gate.power :cartridge,:OFF @21:19:07.59 Carousel.in :default @21:19:07.81 Carousel.home.seek 38 @21:19:08.30 Air.in :unlocking @21:19:08.49 Air.seek :clear @21:19:09.89 Lysis1.in :unlocking @21:19:10.10 Lysis1.seek :clear @21:19:10.18 Plunge.in :unlocking @21:19:10.36 Plunge.seek :clear @21:19:11.59 Carousel.seek 39 @21:19:12.21 Lysis1.in :fast @21:19:12.32 Lysis1.seek :locked @21:19:14.99 Plunge.in :default @21:19:15.11 Plunge.seek :locked @21:19:15.32 Air.in :fast @21:19:15.43 Air.seek :locked @21:19:17.06 Carousel.reconfigure :locked @21:19:17.29 Powering cartridge bus @21:19:19.96 Gate.setIR 1750,5000,0,6 @21:19:20.09 Gate.setIR 0,5000,0,6 @21:19:20.20 Gate.power :cartridge,:OFF @21:19:20.78 Mounted archive Cartridge @21:19:20.96 Toroid.in :hold @21:19:21.08 Toroid.seek :cartridge @21:19:22.95 Toroid.in :default @21:19:23.06 Toroid.seek :clear @21:19:24.92 Waiting for n to answer "record Vacuum" @21:19:28.04 Answered record Vacuum"" @21:19:28.21 Toroid.in :hold @21:19:28.31 Toroid.seek :bypass @21:19:29.99 Toroid.in :default @21:19:30.10 Toroid.seek :clear @21:19:31.76 Waiting for n to answer "recharge if needed" @21:19:34.01 Answered recharge if needed"" @21:19:34.03 Vacuuming cartridge 40 @21:19:34.35 Carousel.in :default @21:19:34.52 Carousel.home.seek 39 @21:19:35.00 Air.in :unlocking @21:19:35.18 Air.seek :clear @21:19:36.57 Lysis1.in :unlocking @21:19:37.46 Lysis1.seek :clear @21:19:37.67 Plunge.in :unlocking @21:19:37.78 Plunge.seek :clear @21:19:38.93 Carousel.seek 40 @21:19:39.52 Lysis1.in :fast @21:19:39.65 Lysis1.seek :locked @21:19:42.33 Plunge.in :default @21:19:42.48 Plunge.seek :locked @21:19:42.64 Air.in :fast @21:19:42.75 Air.seek :locked @21:19:44.36 Carousel.reconfigure :locked @21:19:44.58 Powering cartridge bus @21:19:47.25 Gate.setIR 1750,5000,0,6 @21:19:47.39 Gate.setIR 0,5000,0,6 @21:19:47.50 PuckHeater.configure :default #ambient 24.4C @21:19:47.61 Gate.power :heat,:ON,limit to 5000mA @21:19:48.34 Mounted lysis Cartridge board#Q05058 @21:19:48.42 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:19:48.59 Toroid.in :hold @21:19:48.71 Toroid.seek :cartridge @21:19:50.59 Toroid.in :default @21:19:50.70 Toroid.seek :clear @21:19:52.56 Waiting for n to answer "record Vacuum" @21:19:55.47 Answered record Vacuum"" @21:19:55.66 Toroid.in :hold @21:19:55.77 Toroid.seek :bypass @21:19:57.45 Toroid.in :default @21:19:57.56 Toroid.seek :clear @21:19:59.26 Waiting for n to answer "recharge if needed" @21:20:02.96 Answered recharge if needed"" @21:20:02.98 Vacuuming cartridge 41 @21:20:03.24 Gate.power :heat,:OFF @21:20:03.26 Gate.power :cartridge,:OFF @21:20:03.43 Carousel.in :default @21:20:03.62 Carousel.home.seek 40 @21:20:04.15 Air.in :unlocking @21:20:04.37 Air.seek :clear @21:20:05.84 Lysis1.in :unlocking @21:20:06.04 Lysis1.seek :clear @21:20:06.20 Plunge.in :unlocking @21:20:06.33 Plunge.seek :clear @21:20:07.48 Carousel.seek 41 @21:20:08.08 Lysis1.in :fast @21:20:08.18 Lysis1.seek :locked @21:20:11.45 Plunge.in :default @21:20:11.61 Plunge.seek :locked @21:20:11.80 Air.in :fast @21:20:11.92 Air.seek :locked @21:20:13.53 Carousel.reconfigure :locked @21:20:13.78 Powering cartridge bus @21:20:16.45 Gate.setIR 1750,5000,0,6 @21:20:16.59 Gate.setIR 0,5000,0,6 @21:20:16.69 Gate.power :cartridge,:OFF @21:20:17.29 Mounted archive Cartridge @21:20:17.47 Toroid.in :hold @21:20:17.58 Toroid.seek :cartridge @21:20:19.49 Toroid.in :default @21:20:19.60 Toroid.seek :clear @21:20:21.50 Waiting for n to answer "record Vacuum" @21:20:26.10 Answered record Vacuum"" @21:20:26.26 Toroid.in :hold @21:20:26.37 Toroid.seek :bypass @21:20:28.05 Toroid.in :default @21:20:28.16 Toroid.seek :clear @21:20:29.82 Waiting for n to answer "recharge if needed" @21:20:33.88 Answered recharge if needed"" @21:20:33.90 Vacuuming cartridge 42 @21:20:34.22 Carousel.in :default @21:20:34.40 Carousel.home.seek 41 @21:20:34.91 Air.in :unlocking @21:20:35.07 Air.seek :clear @21:20:36.47 Lysis1.in :unlocking @21:20:36.65 Lysis1.seek :clear @21:20:36.81 Plunge.in :unlocking @21:20:36.93 Plunge.seek :clear @21:20:38.08 Carousel.seek 42 @21:20:38.67 Lysis1.in :fast @21:20:38.78 Lysis1.seek :locked @21:20:41.44 Plunge.in :default @21:20:41.55 Plunge.seek :locked @21:20:41.78 Air.in :fast @21:20:41.89 Air.seek :locked @21:20:43.50 Carousel.reconfigure :locked @21:20:43.75 Powering cartridge bus @21:20:46.42 Gate.setIR 1750,5000,0,6 @21:20:46.55 Gate.setIR 0,5000,0,6 @21:20:46.67 PuckHeater.configure :default #ambient 24.4C @21:20:46.78 Gate.power :heat,:ON,limit to 5000mA @21:20:48.10 Mounted lysis Cartridge board#Q05064 @21:20:48.16 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:20:48.35 Toroid.in :hold @21:20:48.47 Toroid.seek :cartridge @21:20:50.34 Toroid.in :default @21:20:50.45 Toroid.seek :clear @21:20:52.30 Waiting for n to answer "record Vacuum" @21:20:56.32 Answered record Vacuum"" @21:20:56.48 Toroid.in :hold @21:20:56.58 Toroid.seek :bypass @21:20:58.27 Toroid.in :default @21:20:58.38 Toroid.seek :clear @21:21:00.04 Waiting for n to answer "recharge if needed" @21:21:03.58 Answered recharge if needed"" @21:21:03.60 Vacuuming cartridge 43 @21:21:03.91 Gate.power :heat,:OFF @21:21:03.93 Gate.power :cartridge,:OFF @21:21:04.09 Carousel.in :default @21:21:04.26 Carousel.home.seek 42 @21:21:04.76 Air.in :unlocking @21:21:04.95 Air.seek :clear @21:21:06.34 Lysis1.in :unlocking @21:21:06.55 Lysis1.seek :clear @21:21:06.67 Plunge.in :unlocking @21:21:06.81 Plunge.seek :clear @21:21:07.99 Carousel.seek 43 @21:21:08.59 Lysis1.in :fast @21:21:08.71 Lysis1.seek :locked @21:21:11.38 Plunge.in :default @21:21:11.52 Plunge.seek :locked @21:21:11.71 Air.in :fast @21:21:11.81 Air.seek :locked @21:21:13.43 Carousel.reconfigure :locked @21:21:13.67 Powering cartridge bus @21:21:16.35 Gate.setIR 1750,5000,0,6 @21:21:16.48 Gate.setIR 0,5000,0,6 @21:21:16.60 Gate.power :cartridge,:OFF @21:21:17.19 Mounted archive Cartridge @21:21:17.38 Toroid.in :hold @21:21:17.49 Toroid.seek :cartridge @21:21:19.37 Toroid.in :default @21:21:19.48 Toroid.seek :clear @21:21:21.34 Waiting for n to answer "record Vacuum" @21:21:23.85 Answered record Vacuum"" @21:21:24.01 Toroid.in :hold @21:21:24.11 Toroid.seek :bypass @21:21:25.78 Toroid.in :default @21:21:26.49 Toroid.seek :clear @21:21:28.18 Waiting for n to answer "recharge if needed" @21:21:35.32 Answered recharge if needed"" @21:21:35.34 Vacuuming cartridge 44 @21:21:35.67 Carousel.in :default @21:21:35.85 Carousel.home.seek 43 @21:21:36.33 Air.in :unlocking @21:21:36.54 Air.seek :clear @21:21:37.97 Lysis1.in :unlocking @21:21:38.29 Lysis1.seek :clear @21:21:38.37 Plunge.in :unlocking @21:21:38.55 Plunge.seek :clear @21:21:39.78 Carousel.seek 44 @21:21:40.40 Lysis1.in :fast @21:21:40.51 Lysis1.seek :locked @21:21:43.18 Plunge.in :default @21:21:43.33 Plunge.seek :locked @21:21:43.51 Air.in :fast @21:21:43.61 Air.seek :locked @21:21:45.26 Carousel.reconfigure :locked @21:21:45.49 Powering cartridge bus @21:21:48.15 Gate.setIR 1750,5000,0,6 @21:21:48.29 Gate.setIR 0,5000,0,6 @21:21:48.41 PuckHeater.configure :default #ambient 24.4C @21:21:48.51 Gate.power :heat,:ON,limit to 5000mA @21:21:49.21 Mounted lysis Cartridge board#Q05038 @21:21:49.28 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:21:49.45 Toroid.in :hold @21:21:49.57 Toroid.seek :cartridge @21:21:51.48 Toroid.in :default @21:21:51.59 Toroid.seek :clear @21:21:53.49 Waiting for n to answer "record Vacuum" @21:21:56.90 Answered record Vacuum"" @21:21:57.07 Toroid.in :hold @21:21:57.19 Toroid.seek :bypass @21:21:58.86 Toroid.in :default @21:21:58.98 Toroid.seek :clear @21:22:00.64 Waiting for n to answer "recharge if needed" @21:22:04.10 Answered recharge if needed"" @21:22:04.13 Vacuuming cartridge 45 @21:22:04.37 Gate.power :heat,:OFF @21:22:04.42 Gate.power :cartridge,:OFF @21:22:04.55 Carousel.in :default @21:22:04.72 Carousel.home.seek 44 @21:22:05.84 Air.in :unlocking @21:22:06.03 Air.seek :clear @21:22:07.44 Lysis1.in :unlocking @21:22:07.66 Lysis1.seek :clear @21:22:07.81 Plunge.in :unlocking @21:22:07.95 Plunge.seek :clear @21:22:09.10 Carousel.seek 45 @21:22:09.78 Lysis1.in :fast @21:22:09.89 Lysis1.seek :locked @21:22:12.57 Plunge.in :default @21:22:12.71 Plunge.seek :locked @21:22:12.88 Air.in :fast @21:22:12.98 Air.seek :locked @21:22:14.59 Carousel.reconfigure :locked @21:22:14.81 Powering cartridge bus @21:22:17.50 Gate.setIR 1750,5000,0,6 @21:22:17.64 Gate.setIR 0,5000,0,6 @21:22:17.77 Gate.power :cartridge,:OFF @21:22:18.35 Mounted archive Cartridge @21:22:18.55 Toroid.in :hold @21:22:18.66 Toroid.seek :cartridge @21:22:20.55 Toroid.in :default @21:22:20.66 Toroid.seek :clear @21:22:22.52 Waiting for n to answer "record Vacuum" @21:22:25.70 Answered record Vacuum"" @21:22:25.89 Toroid.in :hold @21:22:25.99 Toroid.seek :bypass @21:22:27.71 Toroid.in :default @21:22:27.83 Toroid.seek :clear @21:22:29.49 Waiting for n to answer "recharge if needed" @21:22:31.15 Answered recharge if needed"" @21:22:31.16 Vacuuming cartridge 46 @21:22:31.50 Carousel.in :default @21:22:31.71 Carousel.home.seek 45 @21:22:32.18 Air.in :unlocking @21:22:32.36 Air.seek :clear @21:22:33.78 Lysis1.in :unlocking @21:22:33.99 Lysis1.seek :clear @21:22:34.06 Plunge.in :unlocking @21:22:34.25 Plunge.seek :clear @21:22:35.47 Carousel.seek 46 @21:22:36.67 Lysis1.in :fast @21:22:36.78 Lysis1.seek :locked @21:22:39.45 Plunge.in :default @21:22:39.57 Plunge.seek :locked @21:22:39.80 Air.in :fast @21:22:39.93 Air.seek :locked @21:22:41.54 Carousel.reconfigure :locked @21:22:41.79 Powering cartridge bus @21:22:44.47 Gate.setIR 1750,5000,0,6 @21:22:44.63 Gate.setIR 0,5000,0,6 @21:22:44.74 PuckHeater.configure :default #ambient 24.4C @21:22:44.85 Gate.power :heat,:ON,limit to 5000mA @21:22:45.54 Mounted lysis Cartridge board#Q05076 @21:22:45.64 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:22:45.82 Toroid.in :hold @21:22:45.96 Toroid.seek :cartridge @21:22:47.85 Toroid.in :default @21:22:47.96 Toroid.seek :clear @21:22:49.81 Waiting for n to answer "record Vacuum" @21:22:54.68 Answered record Vacuum"" @21:22:54.85 Toroid.in :hold @21:22:54.95 Toroid.seek :bypass @21:22:56.64 Toroid.in :default @21:22:56.75 Toroid.seek :clear @21:22:58.41 Waiting for n to answer "recharge if needed" @21:23:03.08 Answered recharge if needed"" @21:23:03.09 Vacuuming cartridge 47 @21:23:03.35 Gate.power :heat,:OFF @21:23:03.40 Gate.power :cartridge,:OFF @21:23:03.53 Carousel.in :default @21:23:03.75 Carousel.home.seek 46 @21:23:04.24 Air.in :unlocking @21:23:04.41 Air.seek :clear @21:23:05.81 Lysis1.in :unlocking @21:23:05.99 Lysis1.seek :clear @21:23:06.15 Plunge.in :unlocking @21:23:06.27 Plunge.seek :clear @21:23:07.42 Carousel.seek 47 @21:23:08.01 Lysis1.in :fast @21:23:08.14 Lysis1.seek :locked @21:23:10.80 Plunge.in :default @21:23:10.92 Plunge.seek :locked @21:23:11.13 Air.in :fast @21:23:11.24 Air.seek :locked @21:23:12.85 Carousel.reconfigure :locked @21:23:13.71 Powering cartridge bus @21:23:16.38 Gate.setIR 1750,5000,0,6 @21:23:16.52 Gate.setIR 0,5000,0,6 @21:23:16.63 Gate.power :cartridge,:OFF @21:23:17.22 Mounted archive Cartridge @21:23:17.43 Toroid.in :hold @21:23:17.54 Toroid.seek :cartridge @21:23:19.45 Toroid.in :default @21:23:19.56 Toroid.seek :clear @21:23:21.43 Waiting for n to answer "record Vacuum" @21:23:24.17 Answered record Vacuum"" @21:23:24.33 Toroid.in :hold @21:23:24.46 Toroid.seek :bypass @21:23:26.15 Toroid.in :default @21:23:26.27 Toroid.seek :clear @21:23:27.93 Waiting for n to answer "recharge if needed" @21:23:31.85 Answered recharge if needed"" @21:23:31.87 Vacuuming cartridge 48 @21:23:32.19 Carousel.in :default @21:23:32.37 Carousel.home.seek 47 @21:23:32.86 Air.in :unlocking @21:23:33.04 Air.seek :clear @21:23:34.43 Lysis1.in :unlocking @21:23:34.65 Lysis1.seek :clear @21:23:34.78 Plunge.in :unlocking @21:23:34.89 Plunge.seek :clear @21:23:36.07 Carousel.seek 48 @21:23:36.66 Lysis1.in :fast @21:23:36.77 Lysis1.seek :locked @21:23:39.44 Plunge.in :default @21:23:39.67 Plunge.seek :locked @21:23:39.91 Air.in :fast @21:23:40.02 Air.seek :locked @21:23:41.66 Carousel.reconfigure :locked @21:23:41.89 Powering cartridge bus @21:23:44.56 Gate.setIR 1750,5000,0,6 @21:23:44.69 Gate.setIR 0,5000,0,6 @21:23:44.80 PuckHeater.configure :default #ambient 24.4C @21:23:44.92 Gate.power :heat,:ON,limit to 5000mA @21:23:45.74 Mounted lysis Cartridge board#Q05044 @21:23:45.82 Using this cartridge's heat loss curve for HeatingAccelInCarousel @21:23:46.01 Toroid.in :hold @21:23:46.13 Toroid.seek :cartridge @21:23:48.01 Toroid.in :default @21:23:48.71 Toroid.seek :clear @21:23:50.57 Waiting for n to answer "record Vacuum" @21:23:55.51 Answered record Vacuum"" @21:23:55.70 Toroid.in :hold @21:23:55.81 Toroid.seek :bypass @21:23:57.49 Toroid.in :default @21:23:57.60 Toroid.seek :clear @21:23:59.31 Waiting for n to answer "recharge if needed" @21:24:02.88 Answered recharge if needed"" @21:24:02.91 Vacuuming cartridge 49 @21:24:03.15 Gate.power :heat,:OFF @21:24:03.18 Gate.power :cartridge,:OFF @21:24:03.33 Carousel.in :default @21:24:03.51 Carousel.home.seek 48 @21:24:03.99 Air.in :unlocking @21:24:04.17 Air.seek :clear @21:24:05.56 Lysis1.in :unlocking @21:24:05.79 Lysis1.seek :clear @21:24:05.95 Plunge.in :unlocking @21:24:06.08 Plunge.seek :clear @21:24:07.22 Carousel.seek 49 @21:24:07.84 Lysis1.in :fast @21:24:07.97 Lysis1.seek :locked @21:24:10.64 Plunge.in :default @21:24:10.79 Plunge.seek :locked @21:24:10.97 Air.in :fast @21:24:11.08 Air.seek :locked @21:24:12.71 Carousel.reconfigure :locked @21:24:12.93 Powering cartridge bus @21:24:15.61 Gate.setIR 1750,5000,0,6 @21:24:15.78 Gate.setIR 0,5000,0,6 @21:24:15.89 Gate.power :cartridge,:OFF @21:24:16.47 Mounted archive Cartridge @21:24:16.64 Toroid.in :hold @21:24:16.75 Toroid.seek :cartridge @21:24:18.63 Toroid.in :default @21:24:18.74 Toroid.seek :clear @21:24:20.60 Waiting for n to answer "record Vacuum" @21:24:24.49 Answered record Vacuum"" @21:24:24.65 Toroid.in :hold @21:24:24.75 Toroid.seek :bypass @21:24:26.43 Toroid.in :default @21:24:26.53 Toroid.seek :clear @21:24:28.20 Waiting for n to answer "recharge if needed" @21:24:29.87 Answered recharge if needed"" 1..49 @21:24:52.20 -> SC.to 18 @21:24:52.25 Carousel.seek 18 @21:24:52.60 Slide::Error in n -- Storage Carousel (SC) positionErr at 48 + 101 counts (sensed 49 - 22 counts) @21:25:07.56 -> ESP.ready! @21:25:09.07 Carousel.in :default @21:25:09.19 Carousel.home.jog 0 @21:25:09.69 Toroid.seek :clear @21:25:09.82 Carousel.in :default @21:25:10.00 Carousel.home.seek 49 @21:25:10.50 Carousel.reconfigure :locked @21:25:10.72 Powering cartridge bus @21:25:13.38 Gate.setIR 1750,5000,0,6 @21:25:13.51 Gate.setIR 0,5000,0,6 @21:25:13.64 Gate.power :cartridge,:OFF @21:25:14.23 Mounted archive Cartridge Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) sealed Lysis1 = Lysis Syringe #1 (LyS1) locked Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 75ml Intake is closed Exhaust is opened Bypass = Bypass (PB) is opened Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 49 Pressure = Absolute Pressure (Leak) Sensor @0psia PinPort = Handoff Pin Port (PP) clear @21:25:22.24 -> ESP.reready! @21:25:22.31 Archive.in :default @21:25:22.40 Air.in :default @21:25:22.53 Lysis1.in :default @21:25:22.66 Lysis2.in :default @21:25:22.82 Plunge.in :default @21:25:22.91 Toroid.in :default @21:25:22.99 Archive.home.jog 0 @21:25:23.06 Carousel.in :default @21:25:23.25 PinPort.in :default @21:25:23.37 Air.home.jog 0 @21:25:23.55 Lysis2.home.jog 0 @21:25:23.68 Lysis1.home.jog 0 @21:25:23.80 Plunge.home.jog 0 @21:25:23.87 Toroid.home.jog 0 @21:25:24.04 Carousel.home.jog 0 @21:25:24.17 PinPort.home.jog 0 @21:25:25.20 Lysis2.in :homing @21:25:25.24 Air.in :homing @21:25:25.39 Archive.in :homing @21:25:25.45 Lysis1.in :homing @21:25:25.74 Lysis2.jog 1389 @21:25:25.82 Lysis1.jog 2132 @21:25:25.88 Archive.jog 1380 @21:25:25.98 Air.jog 1957 @21:25:26.36 PinPort.in :homing @21:25:26.40 Toroid.in :homing @21:25:26.45 Plunge.in :homing @21:25:26.69 Toroid.jog 619 @21:25:26.73 Plunge.jog 1556 @21:25:26.79 PinPort.jog -107 @21:25:27.79 PinPort.setRawPosition! 0 @21:25:27.83 Lysis2.setRawPosition! 50 @21:25:27.87 Air.setRawPosition! 50 @21:25:27.94 Lysis1.setRawPosition! 50 @21:25:28.00 Archive.setRawPosition! 50 @21:25:28.31 Lysis2.seek :home @21:25:28.37 PinPort.seek :home @21:25:28.43 Air.seek :home @21:25:28.48 Lysis1.seek :home @21:25:28.63 Archive.seek :home @21:25:29.07 Toroid.setRawPosition! 0 @21:25:29.14 Plunge.setRawPosition! 50 @21:25:29.18 PinPort.in :default @21:25:29.29 Lysis2.in :default @21:25:29.47 Toroid.seek :home @21:25:29.61 Plunge.seek :home @21:25:29.96 Lysis1.in :default @21:25:29.99 Air.in :default @21:25:30.07 Archive.in :default @21:25:30.17 Toroid.in :default @21:25:30.51 Plunge.in :default @21:25:30.90 Toroid.seek :clear @21:25:32.55 Carousel.in :default @21:25:32.73 Carousel.home.seek 49 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 75ml Intake is closed Exhaust is opened Bypass = Bypass (PB) is opened Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 49 Pressure = Absolute Pressure (Leak) Sensor @0psia PinPort = Handoff Pin Port (PP) clear @21:25:51.12 -> SC.to 18 @21:25:51.18 Carousel.seek 18 Storage Carousel (SC) at 18 @21:28:12.22 -> vacToroid 30, 18 @21:28:12.28 Vacuuming protocol for dry cartridges @21:28:13.11 Toroid.seek :clear @21:28:13.25 Carousel.in :default @21:28:13.43 Carousel.home.seek 18 @21:28:13.76 Waiting for n to answer "Disconnect the intake line. push return" @21:28:16.20 Answered Disconnect the intake line. push return"" @21:28:17.03 Toroid.seek :clear @21:28:17.16 Carousel.in :default @21:28:17.34 Carousel.home.seek 18 @21:28:17.69 SamplePump.setPosition! 0ml @21:28:17.79 Intake.open @21:28:18.50 SamplePump.seek 50ml @21:29:19.68 Exhaust.close @21:29:21.07 SamplePump.seek 75ml @21:29:51.87 Intake.close @21:29:52.66 Bypass.close @21:29:53.40 Waiting for n to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @21:29:57.71 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @21:29:57.72 Exhaust.open @21:29:58.53 Bypass.open @21:29:59.31 Waiting for n to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @21:30:01.66 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @21:30:01.68 delay 30 seconds @21:30:31.74 Clearing fluid from cartridge-side of Toroid @21:30:31.90 Toroid.in :hold @21:30:32.00 Toroid.seek :cartridge @21:30:33.89 Toroid.in :default @21:30:34.00 Toroid.seek :clear @21:30:35.84 delay 2 seconds @21:30:37.96 Toroid.in :hold @21:30:38.07 Toroid.seek :bypass @21:30:39.74 Toroid.in :default @21:30:39.86 Toroid.seek :clear @21:30:41.52 Waiting for n to answer "Turn the pump off & load the cartridges. Press Return when loaded." @21:30:49.21 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @21:30:50.08 Toroid.seek :clear @21:30:50.21 Carousel.in :default @21:30:50.39 Carousel.home.seek 18 @21:30:50.72 Waiting for n to answer "Turn the pump on. Push Return" @21:30:52.61 Answered Turn the pump on. Push Return"" @21:30:52.63 Vacuuming individual cartridges. @21:30:53.50 Toroid.seek :clear @21:30:53.66 Carousel.in :default @21:30:53.84 Carousel.home.seek 18 @21:30:54.49 Lysis1.in :fast @21:30:54.60 Lysis1.seek :locked @21:30:57.27 Plunge.seek :locked @21:30:57.47 Air.in :fast @21:30:57.57 Air.seek :locked @21:30:59.25 Carousel.reconfigure :locked @21:30:59.47 Powering cartridge bus @21:31:02.13 Gate.setIR 1750,5000,0,6 @21:31:02.26 Gate.setIR 0,5000,0,6 @21:31:02.37 PuckHeater.configure :default #ambient 24.4C @21:31:02.49 Gate.power :heat,:ON,limit to 5000mA @21:31:03.82 Mounted lysis Cartridge board#Q05057 @21:31:03.87 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:31:03.97 Vacuuming Vent Top cartridge 18 for 30 seconds @21:31:04.00 Air.in :default @21:31:04.13 Air.seek :evac @21:31:17.23 Toroid.in :hold @21:31:17.35 Toroid.seek :cartridge @21:31:19.22 Toroid.in :default @21:31:19.33 Toroid.seek :clear @21:31:21.18 Polling Can every 2:00 @21:31:21.74 PuckHeater.configure :default #ambient 24.3C @21:31:21.84 PuckHeater.seek 40.9C,1:00 @21:31:43.45 PuckHeater.hold 3 seconds,40.9C @21:31:46.68 delayUntil "21:31:50.37" @21:31:50.49 Toroid.in :hold @21:31:50.60 Toroid.seek :bypass @21:31:52.28 Toroid.in :default @21:31:52.44 Toroid.seek :clear @21:31:54.09 delay 2 seconds @21:31:56.12 Air.seek :full @21:32:10.63 Gate.power :heat,:OFF @21:32:10.66 Gate.power :cartridge,:OFF @21:32:10.81 Carousel.in :default @21:32:10.98 Carousel.home.seek 18 @21:32:11.45 Air.in :unlocking @21:32:11.63 Air.seek :clear @21:32:12.71 Lysis1.in :unlocking @21:32:12.89 Lysis1.seek :clear @21:32:13.04 Plunge.in :unlocking @21:32:13.16 Plunge.seek :clear @21:32:14.31 Cartridges under vacuum. Individual Leak Test begins @21:32:14.34 Waiting for n to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @21:32:23.83 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @21:32:23.85 delay 10:00 @21:39:21.50 Session Begun @21:39:23.94 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :dry}, 53 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, sensed: :archive, was: :dry}} @21:40:44.16 -> slot [53..50] =>[:microSPR_phyto, :standard, {lysed:1000,lysed2:1000}] {53..50 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @21:41:02.39 -> Cartridge.reserve 53..50 4 @21:41:05.06 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}} @21:42:23.92 Waiting for n to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @21:43:23.65 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @21:43:23.69 move through each cartridge position & record end vacuum @21:43:23.75 Vacuuming cartridge 18 @21:43:24.16 Carousel.seek 18 @21:43:24.49 Lysis1.in :fast @21:43:24.62 Lysis1.seek :locked @21:43:27.30 Plunge.in :default @21:43:27.46 Plunge.seek :locked @21:43:27.70 Air.in :fast @21:43:27.84 Air.seek :locked @21:43:29.46 Carousel.reconfigure :locked @21:43:29.73 Powering cartridge bus @21:43:32.41 Gate.setIR 1750,5000,0,6 @21:43:32.55 Gate.setIR 0,5000,0,6 @21:43:32.67 PuckHeater.configure :default #ambient 24.3C @21:43:32.79 Gate.power :heat,:ON,limit to 5000mA @21:43:33.49 Mounted lysis Cartridge board#Q05057 @21:43:33.59 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:43:33.81 Toroid.in :hold @21:43:33.95 Toroid.seek :cartridge @21:43:35.86 Toroid.in :default @21:43:35.99 Toroid.seek :clear @21:43:37.86 Waiting for n to answer "record Vacuum" @21:44:07.31 Answered record Vacuum"" @21:44:07.49 Toroid.in :hold @21:44:07.60 Toroid.seek :bypass @21:44:09.34 Toroid.in :default @21:44:09.47 Toroid.seek :clear @21:44:11.14 Waiting for n to answer "recharge if needed" @21:44:22.06 Answered recharge if needed"" @21:44:22.14 Gate.power :heat,:OFF @21:44:22.17 Gate.power :cartridge,:OFF @21:44:22.32 Carousel.in :default @21:44:22.51 Carousel.home.seek 18 @21:44:23.01 Air.in :unlocking @21:44:23.19 Air.seek :clear @21:44:24.62 Lysis1.in :unlocking @21:44:24.82 Lysis1.seek :clear @21:44:25.00 Plunge.in :unlocking @21:44:25.12 Plunge.seek :clear @21:44:26.29 Waiting for n to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @21:45:30.54 UserAbort in n -- User Abort!! @21:45:42.24 -> PB.close;Exhaust.close;Intake.close @21:45:42.27 Bypass.close @21:45:43.02 Exhaust.close Intake is closed @21:46:40.73 -> refillSampleLines @21:46:41.40 Refilling sample & bypass lines with fluid. @21:46:42.36 Toroid.seek :clear @21:46:42.50 Carousel.in :default @21:46:42.68 Carousel.home.seek 18 @21:46:43.02 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @21:46:46.14 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @21:46:46.16 Bypass.open @21:46:46.99 Intake.open @21:46:47.73 SamplePump.setPosition! 0ml @21:46:47.92 SamplePump.seek 30ml @21:47:24.79 Bypass.close @21:47:25.56 Exhaust.open @21:47:26.46 SamplePump.seek 55ml @21:47:57.26 delay 1 second @21:47:58.30 Intake.close @21:47:58.44 Exhaust.close @21:47:59.29 Sampling Loop filled with water @21:48:07.76 -> BACSampler.prime @21:48:07.80 NameError in n -- uninitialized constant BACSampler @21:48:20.28 -> BACsampler.prime @21:48:20.43 Priming sample loop w/150ml, bypass w/50ml @21:48:20.46 SamplePump.setPosition! 0ml @21:48:20.60 Bypass.open @21:48:22.15 Intake.open @21:48:22.94 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:48:25.55 Vacuum tare is 56% of full scale @21:48:25.80 Pressure.in :default @21:48:25.93 Pressure.hold @21:48:26.23 SamplePump.in :prime @21:48:26.37 SamplePump.seek 50ml,2:53 @21:49:27.37 Pressure.stop @21:49:27.47 SamplePump.setPosition! 0ml @21:49:27.57 Exhaust.open @21:49:29.09 Bypass.close @21:49:30.73 Pressure.in :default @21:49:30.85 Pressure.hold @21:49:31.23 SamplePump.in :prime @21:49:31.37 SamplePump.seek 150ml,7:39 @21:52:33.68 Pressure.stop BACsampler @21:54:25.08 -> ESP.shutdown @21:54:25.22 Session Ended @21:54:25.35 Intake.close @21:54:25.41 Exhaust.close @21:54:26.94 Gate.power :motor,:OFF @21:54:26.96 Gate.power :core,:OFF @21:54:27.02 Gate.power :sampler,:OFF @21:54:27.08 Gate.power :analytic1,:OFF @21:54:27.11 Gate.power :analytic2,:OFF @21:54:27.15 Gate.power :main,:OFF [:motor,:core,:sampler,:analytic1,:analytic2,:main] @21:54:35.92 -> MainThread.exit # @21:54:36.04 ThreadError in n -- killed thread @21:54:36.13 Session Ended @21:54:36.19 Session Ended @22:39:58.18PDT06-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @22:40:01.78 >BREAK< ESP3 Gateway boot v1.43 for core bus @22:40:01.81 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @22:40:05.14 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @22:40:05.76 Sea pressure (Abs) tare is 6% of full scale @22:40:13.63 Polling Can every 2:00 @22:40:13.69 Waiting for espclients... @22:55:16.44 Session Begun @22:55:18.76 -> slot {[60, 59, 57, 55, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry, was: :dry}, [58,56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}} @22:55:59.45 -> Cartridge.reserve [57..54] 4 @22:56:18.96 -> Cartridge.reserve [49..1] 49 @22:56:22.16 -> slot {[57,55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [56,54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 58 => { Type: :archiveHiBiomass_bac, State: :dry, was: :dry}, [60,59] => { Type: :microSPR_phyto, State: :dry, was: :dry}} @23:05:04.83 -> fire :dry, :dry, :dry @23:05:04.91 Gate.power :sampler,:OFF,limit to 3500mA @23:05:04.93 Gate.power :main,:ON,limit to 5000mA @23:05:04.98 Gate.power :analytic1,:ON,limit to 5000mA @23:05:05.00 Gate.power :analytic2,:ON,limit to 5000mA @23:05:05.19 Powering core bus @23:05:07.44 SPR Module 1, FW Rev. B, FPGA Rev. C @23:05:07.97 Archive.configure :default @23:05:08.09 Air.configure :default @23:05:08.18 Lysis1.configure :default @23:05:08.28 Lysis2.configure :default @23:05:08.40 Plunge.configure :default @23:05:08.52 SamplePump.configure :default @23:05:08.64 Toroid.configure :default @23:05:08.76 Carousel.configure :default @23:05:08.88 Pressure.configure :default @23:05:09.00 ExtraPump.configure :default @23:05:09.09 PinPort.configure :default @23:05:09.19 All elf controllers running firmware version 4.84 @23:05:09.22 Gate.power :motor,:ON,limit to 5000mA @23:05:09.43 Archive.home.jog 0 @23:05:10.32 PinPort.home.jog 0 @23:05:10.35 Toroid.home.jog 0 @23:05:10.42 Plunge.home.jog 0 @23:05:10.46 Lysis1.home.jog 0 @23:05:10.51 Air.home.jog 0 @23:05:10.55 Carousel.home.jog 0 @23:05:10.58 Lysis2.home.jog 0 @23:05:11.83 Archive.in :homing @23:05:12.19 Archive.jog 1382 @23:05:12.28 Plunge.in :homing @23:05:12.33 Lysis1.in :homing @23:05:12.39 PinPort.in :homing @23:05:12.46 Lysis2.in :homing @23:05:12.50 Air.in :homing @23:05:12.57 Toroid.in :homing @23:05:12.92 Plunge.jog 407 @23:05:12.99 PinPort.jog -106 @23:05:13.03 Lysis1.jog 1386 @23:05:13.08 Lysis2.jog 1390 @23:05:13.12 Air.jog 1360 @23:05:13.17 Toroid.jog 609 @23:05:13.58 PinPort.setRawPosition! 0 @23:05:13.68 PinPort.seek :home @23:05:13.82 Archive.setRawPosition! 50 @23:05:13.87 PinPort.in :default @23:05:14.00 Archive.seek :home @23:05:14.13 Plunge.setRawPosition! 50 @23:05:14.20 Plunge.seek :home @23:05:14.32 Archive.in :default @23:05:14.53 Plunge.in :default @23:05:14.68 Lysis2.setRawPosition! 50 @23:05:14.74 Lysis1.setRawPosition! 50 @23:05:14.81 Air.setRawPosition! 50 @23:05:14.87 Lysis2.seek :home @23:05:14.98 Lysis1.seek :home @23:05:15.07 Air.seek :home @23:05:15.32 Lysis2.in :default @23:05:15.48 Lysis1.in :default @23:05:15.53 Air.in :default @23:05:15.62 Toroid.setRawPosition! 0 @23:05:15.81 Toroid.seek :home @23:05:15.93 Toroid.in :default @23:05:16.06 Toroid.seek :clear @23:05:17.71 Carousel.in :default @23:05:17.88 Carousel.home.seek 19 @23:05:18.27 Selecting Cartridge 60 @23:05:18.62 Carousel.seek 60 @23:05:27.35 Lysis1.in :fast @23:05:27.44 Lysis1.seek :locked @23:05:30.12 Plunge.seek :locked @23:05:30.32 Air.in :fast @23:05:30.41 Air.seek :locked @23:05:32.01 Carousel.reconfigure :locked @23:05:32.20 Powering cartridge bus @23:05:34.87 Gate.setIR 1750,5000,0,6 @23:05:35.03 Gate.setIR 0,5000,0,6 @23:05:35.12 PuckHeater.configure :default @23:05:35.22 Gate.power :heat,:ON,limit to 5000mA @23:05:36.43 Mounted lysis Cartridge board#Q05054 @23:05:36.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:05:36.89 Preparing to filter cartridge 60 @23:05:37.17 Gate.power :sampler,:ON @23:05:37.30 Priming sample loop w/150ml, bypass w/50ml @23:05:37.32 SamplePump.setPosition! 0ml @23:05:37.40 Bypass.open @23:05:38.94 Intake.open @23:05:39.72 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:05:42.31 Vacuum tare is 56% of full scale @23:05:42.50 Pressure.in :default @23:05:42.59 Pressure.hold @23:05:42.85 Waiting for external sampler to finish priming... @23:05:42.87 delayUntil "23:09:07.19" @23:09:07.24 SamplePump.in :prime @23:09:07.35 SamplePump.seek 50ml,2:53 @23:10:08.34 Pressure.stop @23:10:08.47 SamplePump.setPosition! 0ml @23:10:08.55 Exhaust.open @23:10:10.05 Bypass.close @23:10:11.63 Pressure.in :default @23:10:11.73 Pressure.hold @23:10:12.06 SamplePump.in :prime @23:10:12.16 SamplePump.seek 150ml,7:39 @23:13:14.48 Pressure.stop @23:13:14.58 Bypass.open @23:13:15.31 Intake.close @23:13:16.12 Toroid.in :hold @23:13:16.21 Toroid.seek :crack @23:13:17.79 Toroid.seek :cartridge @23:13:18.93 Toroid.in :default @23:13:19.02 Toroid.seek :clear @23:13:21.07 Pressurizing cartridge to 26psi above 14.5psia @23:13:21.10 SamplePump.setPosition! 0ml @23:13:21.19 Intake.open @23:13:21.85 Bypass.close @23:13:22.57 Exhaust.close @23:13:23.42 Pressure.hold @23:13:23.73 SamplePump.in :charge @23:13:23.82 SamplePump.seek 2.3ml,42 seconds @23:13:39.01 Pressure.stop @23:13:39.10 Intake.close @23:13:39.90 Pressurized cartridge with 0.971ml to 26.1psi @23:13:39.92 Isolated @33.8psia (19.3psi) @23:13:39.96 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @23:13:40.01 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:13:44.63 Cartridge pressure fell 2% to 33.2psia @23:13:44.76 Sampling 50.0ml @23:13:44.77 SamplePump.setPosition! 0ml @23:13:44.87 Exhaust.open @23:13:45.62 Intake.open @23:13:46.50 Pressure.in :default @23:13:46.59 Pressure.hold @23:13:46.76 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @23:13:46.98 SamplePump.in :sample @23:13:47.08 SamplePump.seek 50ml,4:41 @23:13:50.12 Cartridge 60 filtering 1/50ml at 0.57ml/s with 18.4psi vacuum @23:14:50.13 Cartridge 60 filtering 35/50ml at 0.55ml/s with 29.7psi vacuum @23:15:18.03 Pressure.stop @23:15:18.21 Intake.close @23:15:18.30 Exhaust.close @23:15:18.53 Toroid.in :hold @23:15:18.63 Toroid.seek :bypass @23:15:20.29 Toroid.in :default @23:15:20.38 Toroid.seek :clear @23:15:22.02 Sampled 50.0ml @23:15:23.72 SPR.command "read_sequences" @23:15:24.00 SPR.command "stats_on" @23:15:24.18 SPR.command "start 0 1" @23:15:24.87 SPR.start /var/log/esp/spr23aug0623h.spr.bz2 @23:15:25.18 Gate.power :sampler,:OFF @23:15:27.35 Handoff.select :waste @23:15:34.09 Syringe tare is 19% of full scale @23:15:34.12 PinPort.in :locking @23:15:34.23 PinPort.seek :locked @23:15:36.82 Archive.in :fast @23:15:36.88 Lysis2.in :fast @23:15:36.98 Lysis1.seek 1350uL @23:15:37.15 Archive.seek :full @23:15:37.23 Lysis2.seek 950uL @23:15:42.01 Plunge.seek :reagent @23:15:42.95 Evacuating puck with filter bubble point of 50psi @23:15:42.98 PinPort.seek :locked @23:15:43.10 Handoff.select :waste @23:15:49.73 Air syringe pumping up to 1 strokes to reach 8psi @23:15:49.74 Air stroke #1 of 1 @23:15:49.88 Air.in :pressurize @23:15:49.97 Air.seek :pressurized @23:15:55.82 4.7psi after pressurize @23:15:55.85 Air.in :compress @23:15:55.96 Air.seek :evac @23:15:59.01 1.9psi after compress @23:16:10.61 0.6psi after compress (stable) @23:16:10.63 Air.in :default @23:16:10.73 Air.hold @23:16:11.03 at 0.5psi Opened to waste @23:16:11.05 Handoff.select :blocked @23:16:11.24 Archive syringe pumping up to 8 strokes to reach 50psi @23:16:11.26 Archive stroke #1 of 8 @23:16:11.40 Archive.in :pressurize @23:16:11.50 Archive.seek :pressurized @23:16:24.03 1.0psi after pressurize @23:16:29.68 1.1psi after pressurize (stable) @23:16:29.79 Archive.in :compress @23:16:29.92 Archive.seek :empty @23:16:44.58 13.6psi after compress @23:16:55.19 14.5psi after compress (stable) @23:16:55.21 Archive.in :gulp @23:16:55.38 Archive.seek :topVent @23:17:01.70 14.7psi after gulp @23:17:07.30 14.7psi after gulp (stable) @23:17:07.32 Archive stroke #2 of 8 @23:17:07.46 Archive.in :pressurize @23:17:07.59 Archive.seek :pressurized @23:17:20.14 14.8psi after pressurize @23:17:40.24 19.1psi after pressurize (stable) @23:17:40.29 Archive.in :compress @23:17:40.40 Archive.seek :empty @23:17:55.06 25.3psi after compress @23:18:05.67 26.3psi after compress (stable) @23:18:05.70 Archive.in :gulp @23:18:05.81 Archive.seek :topVent @23:18:12.10 26.4psi after gulp @23:18:17.71 26.4psi after gulp (stable) @23:18:17.72 Archive stroke #3 of 8 @23:18:17.87 Archive.in :pressurize @23:18:17.97 Archive.seek :pressurized @23:18:30.49 26.4psi after pressurize @23:18:46.59 29.0psi after pressurize (stable) @23:18:46.62 Archive.in :compress @23:18:46.73 Archive.seek :empty @23:19:01.37 37.6psi after compress @23:19:11.99 38.7psi after compress (stable) @23:19:12.02 Archive.in :gulp @23:19:12.14 Archive.seek :topVent @23:19:18.42 38.9psi after gulp @23:19:24.03 38.8psi after gulp (stable) @23:19:24.05 Archive stroke #4 of 8 @23:19:24.19 Archive.in :pressurize @23:19:24.29 Archive.seek :pressurized @23:19:36.81 38.8psi after pressurize @23:19:42.41 38.7psi after pressurize (stable) @23:19:42.44 Archive.in :compress @23:19:43.06 Archive.seek :empty @23:19:57.71 49.3psi after compress @23:19:58.75 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes) @23:19:58.80 Archive.in :default @23:19:58.93 Archive.hold @23:19:59.03 Handoff.select :waste @23:20:11.76 delay 1:00 @23:21:11.79 Handoff.select :blocked @23:21:34.54 Handoff.select :waste @23:21:34.69 0.4psi at evacEnd @23:21:34.70 Handoff.select :blocked @23:21:34.87 Archive.in :fast @23:21:34.96 Archive.seek :topVent @23:21:48.69 Stable @0.5psi after Handoff blocked @23:21:48.72 Archive syringe pumping up to 8 strokes to reach 31psi @23:21:48.75 Archive stroke #1 of 8 @23:21:48.89 Archive.in :pressurize @23:21:48.98 Archive.seek :pressurized @23:22:01.51 1.4psi after pressurize @23:22:01.54 Archive.in :compress @23:22:01.68 Archive.seek :empty @23:22:16.36 11.5psi after compress @23:22:26.97 12.0psi after compress (stable) @23:22:27.00 Archive.in :gulp @23:22:27.11 Archive.seek :topVent @23:22:33.40 12.1psi after gulp @23:22:39.01 12.0psi after gulp (stable) @23:22:39.02 Archive stroke #2 of 8 @23:22:39.17 Archive.in :pressurize @23:22:39.26 Archive.seek :pressurized @23:22:51.80 12.0psi after pressurize @23:22:51.86 Archive.in :compress @23:22:51.98 Archive.seek :empty @23:23:06.65 22.8psi after compress @23:23:17.25 23.3psi after compress (stable) @23:23:17.28 Archive.in :gulp @23:23:17.43 Archive.seek :topVent @23:23:23.72 23.5psi after gulp @23:23:29.32 23.4psi after gulp (stable) @23:23:29.34 Archive stroke #3 of 8 @23:23:29.48 Archive.in :pressurize @23:23:29.58 Archive.seek :pressurized @23:23:42.15 23.3psi after pressurize @23:23:42.18 Archive.in :compress @23:23:42.29 Archive.seek :empty @23:23:52.93 Reached 30.6 of 30.5psi after pumping 5103uL (2.95 strokes) @23:23:52.95 Archive.in :default @23:23:53.07 Archive.hold @23:23:53.17 Handoff.select :waste @23:24:10.94 delay 1:00 @23:25:10.96 Handoff.select :blocked @23:25:33.71 Handoff.select :waste @23:25:33.87 0.4psi at evacEnd @23:25:33.90 Air.seek :fullVent @23:25:35.35 Archive.in :fast @23:25:35.45 Archive.seek :topVent @23:25:42.30 Seawater evacuated, Pressure above/below filter released. @23:25:42.43 Lysis1.in :slow @23:25:42.52 Lysis1.seek 1050uL @23:26:12.55 delay 5 seconds @23:26:17.58 Handoff.select :blocked @23:26:17.83 Lysis1.seek 450uL @23:27:17.76 delay 3 seconds @23:27:20.86 Air.in mix with 1.40048 parts Lysis1 @23:27:21.62 Air.seek :evac @23:27:21.67 Lysis1.seek 300uL @23:27:36.84 Lysis1.seek :empty @23:28:06.96 Lysis buffer delivered @5.2psi @23:28:07.01 Polling Can every 2:00 @23:28:07.61 PuckHeater.configure :default #ambient 25.4C @23:28:07.70 PuckHeater.seek 79.6C @23:28:41.77 PuckHeater.hold 2:00,79.6C @23:30:41.66 Polling Can every 2:00 @23:30:42.22 PuckHeater.configure :default #ambient 25.5C @23:30:42.30 PuckHeater.hold 30 seconds,78.6C @23:31:12.44 Polling Can every 2:00 @23:31:12.99 PuckHeater.configure :default #ambient 25.4C @23:31:13.08 PuckHeater.hold 30 seconds,78.1C @23:31:43.26 Polling Can every 2:00 @23:31:43.82 PuckHeater.configure :default #ambient 25.4C @23:31:43.91 PuckHeater.hold 10:00,77.5C @23:41:42.35 Lysis complete. Syringe pressure @8.5psi @23:41:42.39 Archive syringe pumping up to 5 strokes to reach 40psi @23:41:42.41 Archive stroke #1 of 5 @23:41:42.55 Archive.in :pressurize @23:41:42.64 Archive.seek :pressurized @23:41:55.16 8.5psi after pressurize @23:42:00.76 8.4psi after pressurize (stable) @23:42:00.79 Archive.in :compress @23:42:00.90 Archive.seek :empty @23:42:15.55 28.7psi after compress @23:42:26.15 29.3psi after compress (stable) @23:42:26.17 Archive.in :gulp @23:42:26.29 Archive.seek :topVent @23:42:32.57 29.4psi after gulp @23:42:38.19 29.2psi after gulp (stable) @23:42:38.21 Archive stroke #2 of 5 @23:42:38.40 Archive.in :pressurize @23:42:38.54 Archive.seek :pressurized @23:42:51.07 28.9psi after pressurize @23:43:10.20 32.8psi after pressurize (stable) @23:43:10.22 Archive.in :compress @23:43:10.33 Archive.seek :empty @23:43:20.03 Reached 40.1 of 40.0psi after pumping 3352uL (1.94 strokes) @23:43:20.05 Archive.in :default @23:43:20.16 Archive.hold @23:43:20.81 delay 2 seconds @23:43:22.84 Archive.in :fast @23:43:22.93 Archive.seek :full @23:43:29.78 Lysis1.seek 138uL @23:43:43.68 delay 1 second @23:43:44.72 Archive.seek :full @23:43:44.76 Lysis1.seek :empty @23:43:58.70 Handoff.select :blocked @23:43:58.84 Initial air pressure @41.1psi @23:43:58.95 Lysis1.in :extract @23:43:59.09 Lysis1.seek 600uL,nil @23:44:04.73 Initial 600uL lysate @20.9psi @23:44:04.74 Handoff.select :blocked @23:44:04.89 Initial air pressure @21.0psi @23:44:05.01 Lysis1.seek 825uL,nil @23:44:07.51 Recovered 825uL lysate @16.9psi @23:44:07.54 Air.in :halfSpeed @23:44:07.69 Air.seek :fullVent @23:44:09.93 Puck Top Vented, bottom @9.4psi @23:44:09.95 Handoff.select :waste @23:44:10.12 delay 5 seconds @23:44:15.21 Handoff open to waste, bottom @0.3psi @23:44:15.23 Air.in :default @23:44:15.37 Air.seek :evac @23:44:16.84 Lysis2.in :DAmix @23:44:17.00 Lysis1.in :DAmix @23:44:17.65 Lysis2.seek 700uL @23:44:17.72 Lysis1.seek 575uL @23:44:52.84 delay 5 seconds @23:44:57.87 Handoff.select SPR @23:44:58.05 SPR.command "start 2 14" @23:44:59.33 SPR stabilized @23:44:59.53 delay 10 seconds @23:45:09.55 SPR.command "start 2 0" @23:45:12.70 Waiting up to 11:00 for SPR to enter state 1 @23:47:10.37 SPR.command "hold_on" @23:47:11.76 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:47:12.47 Lysis2.seek 400uL @23:47:12.50 Lysis1.seek 275uL @23:47:54.57 delay 15 seconds @23:48:09.60 SPR.command "hold_off" @23:48:12.41 Waiting up to 38:00 for SPR to enter state 7 @00:02:32.59PDT07-Aug-23 SPRsummary:-23.7RIU,-21.5RIU,-17.5RIU,-15.5RIU,high,high,high after 2987s @00:14:03.02 SPR analysis completed @00:14:11.50 Ended log spr23aug0623h.spr.bz2 @00:14:11.52 SPR.command "stats_off" @00:14:12.08 Selecting Cartridge 59 @00:14:12.30 Gate.power :heat,:OFF @00:14:12.33 Gate.power :cartridge,:OFF @00:14:12.47 PinPort.in :unlocking @00:14:12.56 PinPort.seek :clear @00:14:14.51 Carousel.in :default @00:14:14.68 Carousel.home.seek 60 @00:14:15.07 Archive.in :unlocking @00:14:15.23 Archive.seek :clear @00:14:15.37 Air.in :unlocking @00:14:15.54 Air.seek :clear @00:14:15.74 Lysis2.in :unlocking @00:14:15.91 Lysis2.seek :clear @00:14:19.80 Lysis1.in :unlocking @00:14:20.00 Lysis1.seek :clear @00:14:20.11 Plunge.in :unlocking @00:14:20.21 Plunge.seek :clear @00:14:23.94 Carousel.seek 59 @00:14:24.94 Lysis1.in :fast @00:14:25.08 Lysis1.seek :locked @00:14:27.74 Plunge.in :default @00:14:27.84 Plunge.seek :locked @00:14:28.05 Air.in :fast @00:14:28.13 Air.seek :locked @00:14:29.73 Carousel.reconfigure :locked @00:14:29.92 Powering cartridge bus @00:14:32.59 Gate.setIR 1750,5000,0,6 @00:14:32.72 Gate.setIR 0,5000,0,6 @00:14:32.81 PuckHeater.configure :default #ambient 25.4C @00:14:32.91 Gate.power :heat,:ON,limit to 5000mA @00:14:33.67 Mounted lysis Cartridge board#Q05037 @00:14:33.73 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:14:34.10 Preparing to filter cartridge 59 @00:14:34.39 Gate.power :sampler,:ON @00:14:34.52 Priming sample loop w/150ml, bypass w/50ml @00:14:34.54 SamplePump.setPosition! 0ml @00:14:34.63 Bypass.open @00:14:36.18 Intake.open @00:14:36.96 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:14:39.56 Vacuum tare is 56% of full scale @00:14:39.78 Pressure.in :default @00:14:39.87 Pressure.hold @00:14:40.13 Waiting for external sampler to finish priming... @00:14:40.15 delayUntil "00:18:04.41" @00:18:04.46 SamplePump.in :prime @00:18:04.56 SamplePump.seek 50ml,2:53 @00:19:05.62 Pressure.stop @00:19:05.71 SamplePump.setPosition! 0ml @00:19:05.79 Exhaust.open @00:19:07.25 Bypass.close @00:19:08.82 Pressure.in :default @00:19:08.91 Pressure.hold @00:19:09.24 SamplePump.in :prime @00:19:09.34 SamplePump.seek 150ml,7:39 @00:22:11.47 Pressure.stop @00:22:11.58 Bypass.open @00:22:12.35 Intake.close @00:22:13.17 Toroid.in :hold @00:22:13.27 Toroid.seek :crack @00:22:14.93 Toroid.seek :cartridge @00:22:16.08 Toroid.in :default @00:22:16.17 Toroid.seek :clear @00:22:18.23 Pressurizing cartridge to 26psi above 14.6psia @00:22:18.25 SamplePump.setPosition! 0ml @00:22:18.34 Intake.open @00:22:19.00 Bypass.close @00:22:19.73 Exhaust.close @00:22:20.59 Pressure.in :default @00:22:20.69 Pressure.hold @00:22:20.96 SamplePump.in :charge @00:22:21.06 SamplePump.seek 2.3ml,42 seconds @00:22:35.48 Pressure.stop @00:22:35.60 Intake.close @00:22:36.38 Pressurized cartridge with 0.939ml to 26.3psi @00:22:36.40 Isolated @33.8psia (19.3psi) @00:22:36.42 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @00:22:36.48 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @00:22:41.10 Cartridge pressure fell 1% to 33.5psia @00:22:41.27 Sampling 50.0ml @00:22:41.29 SamplePump.setPosition! 0ml @00:22:41.38 Exhaust.open @00:22:42.10 Intake.open @00:22:42.97 Pressure.in :default @00:22:43.06 Pressure.hold @00:22:43.24 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @00:22:43.47 SamplePump.in :sample @00:22:43.60 SamplePump.seek 50ml,4:41 @00:22:46.59 Cartridge 59 filtering 1/50ml at 0.60ml/s with 27.0psi vacuum @00:23:46.60 Cartridge 59 filtering 34/50ml at 0.48ml/s with 27.2psi vacuum @00:24:17.62 Pressure.stop @00:24:17.80 Intake.close @00:24:17.84 Exhaust.close @00:24:17.99 Handoff.select :close @00:24:18.24 Toroid.in :hold @00:24:18.33 Toroid.seek :bypass @00:24:19.99 Toroid.in :default @00:24:20.08 Toroid.seek :clear @00:24:21.73 Sampled 50.0ml @00:24:22.88 SPR.command "stats_on" @00:24:23.22 SPR.command "start 0 1" @00:24:23.90 SPR.start /var/log/esp/spr23aug0700h.spr.bz2 @00:24:24.90 Gate.power :sampler,:OFF @00:24:26.33 Handoff.select :waste @00:24:33.06 Syringe tare is 19% of full scale @00:24:33.09 PinPort.in :locking @00:24:33.21 PinPort.seek :locked @00:24:35.80 Archive.in :fast @00:24:35.89 Lysis2.in :fast @00:24:35.97 Lysis1.seek 1350uL @00:24:36.09 Archive.seek :full @00:24:36.19 Lysis2.seek 950uL @00:24:40.97 Plunge.seek :reagent @00:24:41.92 Evacuating puck with filter bubble point of 50psi @00:24:41.95 PinPort.seek :locked @00:24:42.13 Handoff.select :waste @00:24:48.75 Air syringe pumping up to 1 strokes to reach 8psi @00:24:48.77 Air stroke #1 of 1 @00:24:48.91 Air.in :pressurize @00:24:49.03 Air.seek :pressurized @00:24:54.81 1.5psi after pressurize @00:24:54.84 Air.in :compress @00:24:54.95 Air.seek :evac @00:24:58.00 1.0psi after compress @00:25:08.61 0.7psi after compress (stable) @00:25:08.63 Air.in :default @00:25:08.73 Air.hold @00:25:09.06 at 0.7psi Opened to waste @00:25:09.07 Handoff.select :blocked @00:25:09.25 Archive syringe pumping up to 8 strokes to reach 50psi @00:25:09.27 Archive stroke #1 of 8 @00:25:09.41 Archive.in :pressurize @00:25:09.50 Archive.seek :pressurized @00:25:22.04 1.3psi after pressurize @00:25:27.67 1.3psi after pressurize (stable) @00:25:27.70 Archive.in :compress @00:25:27.87 Archive.seek :empty @00:25:42.52 14.3psi after compress @00:25:53.12 15.5psi after compress (stable) @00:25:53.15 Archive.in :gulp @00:25:53.25 Archive.seek :topVent @00:25:59.58 15.7psi after gulp @00:26:05.20 15.7psi after gulp (stable) @00:26:05.22 Archive stroke #2 of 8 @00:26:05.36 Archive.in :pressurize @00:26:05.45 Archive.seek :pressurized @00:26:18.03 15.8psi after pressurize @00:26:23.71 15.8psi after pressurize (stable) @00:26:23.75 Archive.in :compress @00:26:23.87 Archive.seek :empty @00:26:38.53 27.8psi after compress @00:26:49.18 28.9psi after compress (stable) @00:26:49.22 Archive.in :gulp @00:26:49.34 Archive.seek :topVent @00:26:55.65 29.1psi after gulp @00:27:01.25 29.0psi after gulp (stable) @00:27:01.27 Archive stroke #3 of 8 @00:27:01.42 Archive.in :pressurize @00:27:01.54 Archive.seek :pressurized @00:27:14.09 29.0psi after pressurize @00:27:19.69 28.9psi after pressurize (stable) @00:27:19.72 Archive.in :compress @00:27:19.83 Archive.seek :empty @00:27:34.49 40.9psi after compress @00:27:45.14 42.4psi after compress (stable) @00:27:45.17 Archive.in :gulp @00:27:45.28 Archive.seek :topVent @00:27:51.62 42.6psi after gulp @00:27:57.27 42.5psi after gulp (stable) @00:27:57.30 Archive stroke #4 of 8 @00:27:57.44 Archive.in :pressurize @00:27:57.53 Archive.seek :pressurized @00:28:10.09 42.4psi after pressurize @00:28:15.69 42.3psi after pressurize (stable) @00:28:15.71 Archive.in :compress @00:28:15.83 Archive.seek :empty @00:28:27.99 Reached 50.2 of 50.0psi after pumping 6865uL (3.97 strokes) @00:28:28.01 Archive.in :default @00:28:28.11 Archive.hold @00:28:28.22 Handoff.select :waste @00:28:43.47 delay 1:00 @00:29:43.49 Handoff.select :blocked @00:30:06.23 Handoff.select :waste @00:30:06.39 0.4psi at evacEnd @00:30:06.41 Handoff.select :blocked @00:30:06.58 Archive.in :fast @00:30:06.67 Archive.seek :topVent @00:30:20.21 Stable @0.5psi after Handoff blocked @00:30:20.24 Archive syringe pumping up to 8 strokes to reach 31psi @00:30:20.28 Archive stroke #1 of 8 @00:30:20.42 Archive.in :pressurize @00:30:20.51 Archive.seek :pressurized @00:30:33.03 0.6psi after pressurize @00:30:33.06 Archive.in :compress @00:30:33.17 Archive.seek :empty @00:30:47.83 11.2psi after compress @00:30:58.42 12.2psi after compress (stable) @00:30:58.45 Archive.in :gulp @00:30:58.61 Archive.seek :topVent @00:31:04.90 12.4psi after gulp @00:31:10.50 12.3psi after gulp (stable) @00:31:10.52 Archive stroke #2 of 8 @00:31:10.66 Archive.in :pressurize @00:31:10.75 Archive.seek :pressurized @00:31:23.27 12.3psi after pressurize @00:31:23.30 Archive.in :compress @00:31:23.46 Archive.seek :empty @00:31:38.10 23.1psi after compress @00:31:48.83 24.0psi after compress (stable) @00:31:48.86 Archive.in :gulp @00:31:49.02 Archive.seek :topVent @00:31:55.30 24.1psi after gulp @00:32:00.90 24.1psi after gulp (stable) @00:32:00.92 Archive stroke #3 of 8 @00:32:01.05 Archive.in :pressurize @00:32:01.15 Archive.seek :pressurized @00:32:13.71 24.1psi after pressurize @00:32:13.73 Archive.in :compress @00:32:13.86 Archive.seek :empty @00:32:24.09 Reached 30.6 of 30.5psi after pumping 5094uL (2.94 strokes) @00:32:24.11 Archive.in :default @00:32:24.23 Archive.hold @00:32:24.33 Handoff.select :waste @00:32:42.57 delay 1:00 @00:33:42.59 Handoff.select :blocked @00:34:05.34 Handoff.select :waste @00:34:05.50 0.9psi at evacEnd @00:34:05.53 Air.seek :fullVent @00:34:07.04 Archive.in :fast @00:34:07.16 Archive.seek :topVent @00:34:13.97 Seawater evacuated, Pressure above/below filter released. @00:34:14.10 Lysis1.in :slow @00:34:14.19 Lysis1.seek 1050uL @00:34:44.23 delay 5 seconds @00:34:49.25 Handoff.select :blocked @00:34:49.49 Lysis1.seek 450uL @00:35:49.45 delay 3 seconds @00:35:52.55 Air.in mix with 1.40048 parts Lysis1 @00:35:53.23 Air.seek :evac @00:35:53.30 Lysis1.seek 300uL @00:36:08.45 Lysis1.seek :empty @00:36:38.58 Lysis buffer delivered @5.5psi @00:36:38.60 Polling Can every 2:00 @00:36:39.18 PuckHeater.configure :default #ambient 25.8C @00:36:39.26 PuckHeater.seek 79.6C @00:37:18.72 PuckHeater.hold 2:00,79.6C @00:39:18.40 Polling Can every 2:00 @00:39:18.95 PuckHeater.hold 30 seconds,78.6C @00:39:49.05 Polling Can every 2:00 @00:39:49.63 PuckHeater.configure :default #ambient 25.8C @00:39:49.72 PuckHeater.hold 30 seconds,78C @00:40:19.82 Polling Can every 2:00 @00:40:20.38 PuckHeater.configure :default #ambient 25.7C @00:40:20.47 PuckHeater.hold 10:00,77.5C @00:50:17.91 Lysis complete. Syringe pressure @7.4psi @00:50:17.96 Archive syringe pumping up to 5 strokes to reach 40psi @00:50:17.97 Archive stroke #1 of 5 @00:50:18.10 Archive.in :pressurize @00:50:18.20 Archive.seek :pressurized @00:50:30.79 7.4psi after pressurize @00:50:36.46 7.3psi after pressurize (stable) @00:50:36.48 Archive.in :compress @00:50:36.59 Archive.seek :empty @00:50:51.24 25.4psi after compress @00:51:01.85 26.5psi after compress (stable) @00:51:01.89 Archive.in :gulp @00:51:02.03 Archive.seek :topVent @00:51:08.31 26.4psi after gulp @00:51:13.91 26.3psi after gulp (stable) @00:51:13.93 Archive stroke #2 of 5 @00:51:14.06 Archive.in :pressurize @00:51:14.15 Archive.seek :pressurized @00:51:26.75 26.2psi after pressurize @00:51:32.35 26.0psi after pressurize (stable) @00:51:32.37 Archive.in :compress @00:51:32.50 Archive.seek :empty @00:51:46.61 Reached 40.4 of 40.0psi after pumping 3448uL (1.99 strokes) @00:51:46.63 Archive.in :default @00:51:46.73 Archive.hold @00:51:46.83 delay 2 seconds @00:51:48.86 Archive.in :fast @00:51:48.95 Archive.seek :full @00:51:56.12 Lysis1.seek 138uL @00:52:10.51 delay 1 second @00:52:11.56 Archive.seek :full @00:52:11.64 Lysis1.seek :empty @00:52:25.62 Handoff.select :blocked @00:52:25.77 Initial air pressure @42.0psi @00:52:25.87 Lysis1.in :extract @00:52:26.03 Lysis1.seek 600uL,nil @00:52:31.72 Initial 600uL lysate @23.5psi @00:52:31.77 Handoff.select :blocked @00:52:31.94 Initial air pressure @23.3psi @00:52:32.04 Lysis1.seek 825uL,nil @00:52:34.49 Recovered 825uL lysate @19.4psi @00:52:34.52 Air.in :halfSpeed @00:52:34.67 Air.seek :fullVent @00:52:37.47 Puck Top Vented, bottom @9.3psi @00:52:37.50 Handoff.select :waste @00:52:37.69 delay 5 seconds @00:52:42.78 Handoff open to waste, bottom @0.3psi @00:52:42.81 Air.in :default @00:52:42.92 Air.seek :evac @00:52:44.37 Lysis2.in :DAmix @00:52:44.53 Lysis1.in :DAmix @00:52:45.11 Lysis2.seek 700uL @00:52:45.17 Lysis1.seek 575uL @00:53:20.30 delay 5 seconds @00:53:25.33 Handoff.select SPR @00:53:25.49 SPR.command "start 2 14" @00:53:26.13 SPR stabilized @00:53:26.32 delay 10 seconds @00:53:36.34 SPR.command "start 2 0" @00:53:39.50 Waiting up to 11:00 for SPR to enter state 1 @00:55:37.17 SPR.command "hold_on" @00:55:38.55 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:55:39.25 Lysis2.seek 400uL @00:55:39.30 Lysis1.seek 275uL @00:56:21.35 delay 15 seconds @00:56:36.37 SPR.command "hold_off" @00:56:39.21 Waiting up to 38:00 for SPR to enter state 7 @01:10:59.39 SPRsummary:-18.6RIU,4e+09RIU,14.6RIU,61RIU,none,high,419ng/L after 6675s @01:22:29.83 SPR analysis completed @01:22:38.52 Ended log spr23aug0700h.spr.bz2 @01:22:38.54 SPR.command "stats_off" @01:22:38.88 Selecting Cartridge 58 @01:22:39.09 Gate.power :heat,:OFF @01:22:39.12 Gate.power :cartridge,:OFF @01:22:39.25 PinPort.in :unlocking @01:22:39.35 PinPort.seek :clear @01:22:41.30 Carousel.in :default @01:22:41.46 Carousel.home.seek 59 @01:22:41.88 Archive.in :unlocking @01:22:42.07 Archive.seek :clear @01:22:42.16 Air.in :unlocking @01:22:42.36 Air.seek :clear @01:22:42.50 Lysis2.in :unlocking @01:22:42.71 Lysis2.seek :clear @01:22:46.58 Lysis1.in :unlocking @01:22:46.78 Lysis1.seek :clear @01:22:46.83 Plunge.in :unlocking @01:22:46.98 Plunge.seek :clear @01:22:50.74 Carousel.seek 58 @01:22:51.34 Lysis1.in :fast @01:22:51.45 Lysis1.seek :locked @01:22:54.11 Plunge.in :default @01:22:54.24 Plunge.seek :locked @01:22:54.40 Air.in :fast @01:22:54.49 Air.seek :locked @01:22:56.08 Carousel.reconfigure :locked @01:22:56.29 Powering cartridge bus @01:22:58.96 Gate.setIR 1750,5000,0,6 @01:22:59.09 Gate.setIR 0,5000,0,6 @01:22:59.19 Gate.power :cartridge,:OFF @01:22:59.76 Mounted archive Cartridge @01:23:00.14 Preparing to filter cartridge 58 @01:23:00.42 Gate.power :sampler,:ON @01:23:00.54 Priming sample loop w/150ml, bypass w/50ml @01:23:00.56 SamplePump.setPosition! 0ml @01:23:00.64 Bypass.open @01:23:02.20 Intake.open @01:23:03.00 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:23:05.62 Vacuum tare is 56% of full scale @01:23:05.82 Pressure.in :default @01:23:05.90 Pressure.hold @01:23:06.15 Waiting for external sampler to finish priming... @01:23:06.17 delayUntil "01:26:30.44" @01:26:30.49 SamplePump.in :prime @01:26:30.60 SamplePump.seek 50ml,2:53 @01:27:31.62 Pressure.stop @01:27:31.70 SamplePump.setPosition! 0ml @01:27:31.78 Exhaust.open @01:27:33.29 Bypass.close @01:27:34.86 Pressure.in :default @01:27:34.95 Pressure.hold @01:27:35.29 SamplePump.in :prime @01:27:35.39 SamplePump.seek 150ml,7:39 @01:30:38.27 Pressure.stop @01:30:38.43 Bypass.open @01:30:39.18 Intake.close @01:30:39.99 Toroid.in :hold @01:30:40.09 Toroid.seek :crack @01:30:41.66 Toroid.seek :cartridge @01:30:42.80 Toroid.in :default @01:30:42.90 Toroid.seek :clear @01:30:44.95 Pressurizing cartridge to 26psi above 14.7psia @01:30:44.97 SamplePump.setPosition! 0ml @01:30:45.06 Intake.open @01:30:45.71 Bypass.close @01:30:46.43 Exhaust.close @01:30:47.27 Pressure.in :default @01:30:47.36 Pressure.hold @01:30:47.66 SamplePump.in :charge @01:30:47.76 SamplePump.seek 2.3ml,42 seconds @01:30:54.96 Pressure.stop @01:30:55.05 Intake.close @01:30:55.79 Pressurized cartridge with 0.229ml to 26.3psi @01:30:55.82 Isolated @34.6psia (19.9psi) @01:30:55.85 Verifying cartridge holds at least 0.65..0.85 of 34.6psia @01:30:55.91 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @01:31:00.53 Cartridge pressure fell 1% to 34.5psia @01:31:00.65 Sampling 1000.0ml @01:31:00.67 SamplePump.setPosition! 0ml @01:31:00.75 Exhaust.open @01:31:01.47 Intake.open @01:31:02.32 Pressure.in :default @01:31:02.41 Pressure.hold @01:31:02.59 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @01:31:02.80 SamplePump.in :sample @01:31:02.90 SamplePump.seek 1000ml,1:23:51 @01:31:05.94 Cartridge 58 filtering 1/1000ml at 0.55ml/s with 18.8psi vacuum @01:32:05.94 Cartridge 58 filtering 36/1000ml at 0.59ml/s with 22.9psi vacuum @01:33:05.94 Cartridge 58 filtering 68/1000ml at 0.54ml/s with 16.7psi vacuum 1.7m deep (17.2psia) @01:34:06.45 Cartridge 58 filtering 99/1000ml at 0.50ml/s with 24.6psi vacuum @01:35:05.94 Cartridge 58 filtering 128/1000ml at 0.50ml/s with 27.4psi vacuum @01:36:05.94 Cartridge 58 filtering 158/1000ml at 0.46ml/s with 14.2psi vacuum @01:37:05.94 Cartridge 58 filtering 187/1000ml at 0.46ml/s with 11.1psi vacuum @01:38:05.94 Cartridge 58 filtering 215/1000ml at 0.46ml/s with 10.7psi vacuum @01:39:05.94 Cartridge 58 filtering 244/1000ml at 0.43ml/s with 29.0psi vacuum @01:40:05.95 Cartridge 58 filtering 272/1000ml at 0.43ml/s with 28.3psi vacuum @01:41:05.94 Cartridge 58 filtering 299/1000ml at 0.42ml/s with 19.5psi vacuum @01:42:05.93 Cartridge 58 filtering 327/1000ml at 0.49ml/s with 25.9psi vacuum @01:43:05.94 Cartridge 58 filtering 354/1000ml at 0.44ml/s with 32.3psi vacuum @01:44:05.94 Cartridge 58 filtering 381/1000ml at 0.48ml/s with 24.6psi vacuum @01:45:05.94 Cartridge 58 filtering 408/1000ml at 0.47ml/s with 20.4psi vacuum 2.8m deep (18.8psia) @01:46:05.95 Cartridge 58 filtering 435/1000ml at 0.45ml/s with 12.2psi vacuum 3.9m deep (20.4psia) @01:47:05.94 Cartridge 58 filtering 461/1000ml at 0.40ml/s with 25.1psi vacuum @01:48:05.93 Cartridge 58 filtering 488/1000ml at 0.46ml/s with 27.5psi vacuum @01:49:05.95 Cartridge 58 filtering 514/1000ml at 0.47ml/s with 17.1psi vacuum 4.2m deep (21.0psia) @01:50:05.94 Cartridge 58 filtering 541/1000ml at 0.45ml/s with 28.5psi vacuum @01:51:05.94 Cartridge 58 filtering 567/1000ml at 0.45ml/s with 30.0psi vacuum @01:52:05.95 Cartridge 58 filtering 593/1000ml at 0.44ml/s with 28.7psi vacuum @01:53:05.94 Cartridge 58 filtering 618/1000ml at 0.39ml/s with 30.9psi vacuum @01:54:05.94 Cartridge 58 filtering 644/1000ml at 0.38ml/s with 24.0psi vacuum @01:55:05.93 Cartridge 58 filtering 670/1000ml at 0.41ml/s with 26.3psi vacuum @01:56:05.94 Cartridge 58 filtering 695/1000ml at 0.43ml/s with 23.1psi vacuum 2.3m deep (18.0psia) @01:57:05.94 Cartridge 58 filtering 720/1000ml at 0.41ml/s with 19.3psi vacuum @01:58:05.95 Cartridge 58 filtering 745/1000ml at 0.45ml/s with 27.8psi vacuum @01:59:05.94 Cartridge 58 filtering 770/1000ml at 0.43ml/s with 30.4psi vacuum @02:00:05.94 Cartridge 58 filtering 795/1000ml at 0.44ml/s with 27.6psi vacuum @02:01:05.95 Cartridge 58 filtering 820/1000ml at 0.38ml/s with 29.9psi vacuum @02:02:05.94 Cartridge 58 filtering 844/1000ml at 0.40ml/s with 25.3psi vacuum @02:03:05.94 Cartridge 58 filtering 869/1000ml at 0.41ml/s with 13.3psi vacuum 1.8m deep (17.4psia) @02:04:05.95 Cartridge 58 filtering 893/1000ml at 0.44ml/s with 24.8psi vacuum @02:05:05.94 Cartridge 58 filtering 918/1000ml at 0.39ml/s with 13.0psi vacuum @02:06:05.94 Cartridge 58 filtering 942/1000ml at 0.41ml/s with 30.6psi vacuum @02:07:06.47 Cartridge 58 filtering 966/1000ml at 0.41ml/s with 16.6psi vacuum 2.4m deep (18.2psia) @02:08:05.94 Cartridge 58 filtering 990/1000ml at 0.35ml/s with 27.5psi vacuum @02:08:31.90 Pressure.stop @02:08:32.12 Intake.close @02:08:32.18 Exhaust.close @02:08:32.28 Handoff.select :close @02:08:32.67 Toroid.in :hold @02:08:32.76 Toroid.seek :bypass @02:08:34.42 Toroid.in :default @02:08:34.51 Toroid.seek :clear @02:08:36.16 Sampled 1000.0ml @02:08:36.86 Plunge.seek :reagent @02:08:37.70 Sample loop closed. Ready to Deliver Reagents @02:08:37.72 Archive.in :default @02:08:37.84 Archive.seek :sealed @02:08:39.32 Gate.power :sampler,:OFF @02:08:41.78 Archive.seek :evac @02:08:55.85 delay 1:00 @02:09:55.89 Archive.seek :clear @02:10:13.39 RNAlater soak for 10:00 @02:20:13.43 Archive.seek :tVent @02:20:15.91 delay 1 second @02:20:16.93 Archive.seek :evac @02:20:32.46 delay 10 seconds @02:20:42.50 Archive.seek :tVent @02:20:58.03 delay 1 second @02:20:59.06 Archive.seek :evac @02:21:14.59 delay 10 seconds @02:21:24.62 Sample preserved and evacuated @02:21:24.94 Carousel.in :default @02:21:25.11 Carousel.home.seek 58 @02:21:25.48 Plunge.seek :locked @02:21:26.40 Archive.in :unlocking @02:21:26.56 Archive.seek :clear @02:21:26.70 Air.in :unlocking @02:21:26.90 Air.seek :clear @02:21:26.98 Lysis1.in :unlocking @02:21:27.21 Lysis1.seek :clear @02:21:31.15 Gate.power :motor,:OFF @02:21:31.17 Gate.power :core,:OFF @02:21:31.21 Gate.power :analytic1,:OFF @02:21:31.76 Gate.power :analytic2,:OFF @02:21:31.81 Gate.power :main,:OFF @02:21:31.85 Safely stopped and ready to power off :STOPPED @04:33:25.90 -> Cartridge.release [57,56] 2 @04:33:47.48 -> fire :dry,:dry @04:33:47.56 Gate.power :sampler,:OFF,limit to 3500mA @04:33:47.60 Gate.power :main,:ON,limit to 5000mA @04:33:47.65 Gate.power :analytic1,:ON,limit to 5000mA @04:33:47.69 Gate.power :analytic2,:ON,limit to 5000mA @04:33:47.79 Powering core bus @04:33:50.14 SPR Module 1, FW Rev. B, FPGA Rev. C @04:33:50.57 Archive.configure :default @04:33:50.70 Air.configure :default @04:33:50.79 Lysis1.configure :default @04:33:50.89 Lysis2.configure :default @04:33:51.00 Plunge.configure :default @04:33:51.13 SamplePump.configure :default @04:33:51.26 Toroid.configure :default @04:33:51.38 Carousel.configure :default @04:33:51.50 Pressure.configure :default @04:33:51.65 ExtraPump.configure :default @04:33:51.74 PinPort.configure :default @04:33:51.83 All elf controllers running firmware version 4.84 @04:33:51.86 Gate.power :motor,:ON,limit to 5000mA @04:33:52.08 Archive.home.jog 0 @04:33:52.96 PinPort.home.jog 0 @04:33:53.00 Toroid.home.jog 0 @04:33:53.07 Plunge.home.jog 0 @04:33:53.13 Lysis1.home.jog 0 @04:33:53.17 Air.home.jog 0 @04:33:53.21 Carousel.home.jog 0 @04:33:53.26 Lysis2.home.jog 0 @04:33:54.11 Archive.in :homing @04:33:54.53 Archive.jog 1390 @04:33:54.62 Toroid.in :homing @04:33:54.66 Lysis2.in :homing @04:33:54.73 Air.in :homing @04:33:54.78 Plunge.in :homing @04:33:54.84 Lysis1.in :homing @04:33:54.90 PinPort.in :homing @04:33:55.27 Toroid.jog 618 @04:33:55.34 Lysis2.jog 1388 @04:33:55.40 Air.jog 1359 @04:33:55.45 Plunge.jog 1568 @04:33:55.50 PinPort.jog -107 @04:33:55.62 Lysis1.jog 1387 @04:33:56.06 PinPort.setRawPosition! 0 @04:33:56.15 PinPort.seek :home @04:33:56.72 Archive.setRawPosition! 50 @04:33:56.84 PinPort.in :default @04:33:56.99 Archive.seek :home @04:33:57.20 Lysis2.setRawPosition! 50 @04:33:57.25 Lysis1.setRawPosition! 50 @04:33:57.28 Plunge.setRawPosition! 50 @04:33:57.33 Air.setRawPosition! 50 @04:33:57.51 Lysis2.seek :home @04:33:57.58 Lysis1.seek :home @04:33:57.77 Air.seek :home @04:33:57.82 Plunge.seek :home @04:33:58.13 Toroid.setRawPosition! 0 @04:33:58.16 Lysis2.in :default @04:33:58.21 Archive.in :default @04:33:58.41 Toroid.seek :home @04:33:58.62 Plunge.in :default @04:33:58.68 Lysis1.in :default @04:33:58.74 Toroid.in :default @04:33:58.82 Air.in :default @04:33:59.30 Toroid.seek :clear @04:34:00.94 Carousel.in :default @04:34:01.10 Carousel.home.seek 58 @04:34:01.40 Selecting Cartridge 57 @04:34:01.80 Carousel.seek 57 @04:34:02.38 Lysis1.in :fast @04:34:02.48 Lysis1.seek :locked @04:34:05.15 Plunge.seek :locked @04:34:05.36 Air.in :fast @04:34:05.46 Air.seek :locked @04:34:07.07 Carousel.reconfigure :locked @04:34:07.32 Powering cartridge bus @04:34:10.01 Gate.setIR 1750,5000,0,6 @04:34:10.14 Gate.setIR 0,5000,0,6 @04:34:10.23 PuckHeater.configure :default #ambient 25.7C @04:34:10.32 Gate.power :heat,:ON,limit to 5000mA @04:34:11.03 Mounted lysis Cartridge board#Q05048 @04:34:11.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @04:34:11.45 Preparing to filter cartridge 57 @04:34:11.76 Gate.power :sampler,:ON @04:34:11.89 Priming sample loop w/150ml, bypass w/50ml @04:34:11.91 SamplePump.setPosition! 0ml @04:34:11.99 Bypass.open @04:34:13.55 Intake.open @04:34:14.88 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:34:17.48 Vacuum tare is 56% of full scale @04:34:17.69 Pressure.in :default @04:34:17.78 Pressure.hold @04:34:18.04 Waiting for external sampler to finish priming... @04:34:18.06 delayUntil "04:37:41.78" @04:37:41.83 SamplePump.in :prime @04:37:41.94 SamplePump.seek 50ml,2:53 @04:38:42.98 Pressure.stop @04:38:43.06 SamplePump.setPosition! 0ml @04:38:43.15 Exhaust.open @04:38:44.64 Bypass.close @04:38:46.21 Pressure.in :default @04:38:46.30 Pressure.hold @04:38:46.64 SamplePump.in :prime @04:38:46.74 SamplePump.seek 150ml,7:39 @04:41:49.18 Pressure.stop @04:41:49.29 Bypass.open @04:41:50.07 Intake.close @04:41:50.88 Toroid.in :hold @04:41:50.98 Toroid.seek :crack @04:41:52.55 Toroid.seek :cartridge @04:41:53.69 Toroid.in :default @04:41:53.78 Toroid.seek :clear @04:41:55.86 Pressurizing cartridge to 26psi above 14.7psia @04:41:55.88 SamplePump.setPosition! 0ml @04:41:55.97 Intake.open @04:41:56.64 Bypass.close @04:41:57.33 Exhaust.close @04:41:58.20 Pressure.in :default @04:41:58.29 Pressure.hold @04:41:58.56 SamplePump.in :charge @04:41:58.65 SamplePump.seek 2.3ml,42 seconds @04:42:12.08 Pressure.stop @04:42:12.18 Intake.close @04:42:12.94 Pressurized cartridge with 0.812ml to 26.1psi @04:42:12.96 Isolated @34.0psia (19.3psi) @04:42:12.98 Verifying cartridge holds at least 0.65..0.85 of 34.0psia @04:42:13.03 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @04:42:17.67 Cartridge pressure fell 1% to 33.8psia @04:42:17.80 Sampling 50.0ml @04:42:17.82 SamplePump.setPosition! 0ml @04:42:17.91 Exhaust.open @04:42:18.69 Intake.open @04:42:19.54 Pressure.in :default @04:42:19.67 Pressure.hold @04:42:19.85 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @04:42:20.07 SamplePump.in :sample @04:42:20.17 SamplePump.seek 50ml,4:41 @04:42:23.20 Cartridge 57 filtering 1/50ml at 0.61ml/s with 25.9psi vacuum @04:43:23.21 Cartridge 57 filtering 36/50ml at 0.56ml/s with 22.0psi vacuum @04:43:49.52 Pressure.stop @04:43:49.74 Intake.close @04:43:49.81 Exhaust.close @04:43:50.06 Toroid.in :hold @04:43:50.16 Toroid.seek :bypass @04:43:51.83 Toroid.in :default @04:43:51.94 Toroid.seek :clear @04:43:53.61 Sampled 50.0ml @04:43:55.33 SPR.command "read_sequences" @04:43:55.40 SPR.command "stats_on" @04:43:55.58 SPR.command "start 0 1" @04:43:56.28 SPR.start /var/log/esp/spr23aug0704h.spr.bz2 @04:43:56.77 Gate.power :sampler,:OFF @04:43:58.18 Handoff.select :waste @04:44:04.91 Syringe tare is 18% of full scale @04:44:04.96 PinPort.in :locking @04:44:05.05 PinPort.seek :locked @04:44:07.65 Archive.in :fast @04:44:07.72 Lysis2.in :fast @04:44:07.85 Lysis1.seek 1350uL @04:44:07.99 Archive.seek :full @04:44:08.15 Lysis2.seek 950uL @04:44:12.90 Plunge.seek :reagent @04:44:13.90 Evacuating puck with filter bubble point of 50psi @04:44:13.93 PinPort.seek :locked @04:44:14.07 Handoff.select :waste @04:44:20.69 Air syringe pumping up to 1 strokes to reach 8psi @04:44:20.71 Air stroke #1 of 1 @04:44:20.84 Air.in :pressurize @04:44:20.93 Air.seek :pressurized @04:44:26.71 0.1psi after pressurize @04:44:26.73 Air.in :compress @04:44:26.85 Air.seek :evac @04:44:29.95 0.3psi after compress @04:44:40.61 0.2psi after compress (stable) @04:44:40.63 Air.in :default @04:44:40.74 Air.hold @04:44:40.99 at 0.3psi Opened to waste @04:44:41.01 Handoff.select :blocked @04:44:41.21 Archive syringe pumping up to 8 strokes to reach 50psi @04:44:41.24 Archive stroke #1 of 8 @04:44:41.40 Archive.in :pressurize @04:44:41.49 Archive.seek :pressurized @04:44:54.02 9.6psi after pressurize @04:44:59.65 9.6psi after pressurize (stable) @04:44:59.67 Archive.in :compress @04:44:59.79 Archive.seek :empty @04:45:14.45 25.2psi after compress @04:45:25.56 27.0psi after compress (stable) @04:45:25.62 Archive.in :gulp @04:45:25.77 Archive.seek :topVent @04:45:32.06 28.3psi after gulp @04:45:37.67 28.3psi after gulp (stable) @04:45:37.69 Archive stroke #2 of 8 @04:45:37.83 Archive.in :pressurize @04:45:37.93 Archive.seek :pressurized @04:45:50.48 27.5psi after pressurize @04:45:56.28 27.6psi after pressurize (stable) @04:45:56.31 Archive.in :compress @04:45:56.43 Archive.seek :empty @04:46:07.42 Session Begun @04:46:09.40 -> slot {[55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 56 => { Type: :archiveHiBiomass_bac, State: :dry}, 57 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug0704h.spr.bz2}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0700h.spr.bz2, was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0623h.spr.bz2, was: :dry}} @04:46:11.13 39.9psi after compress @04:46:25.28 45.5psi after compress (stable) @04:46:25.33 Archive.in :gulp @04:46:25.48 Archive.seek :topVent @04:46:31.77 46.3psi after gulp @04:46:37.37 46.1psi after gulp (stable) @04:46:37.39 Archive stroke #3 of 8 @04:46:37.58 Archive.in :pressurize @04:46:37.77 Archive.seek :pressurized @04:46:50.31 45.1psi after pressurize @04:46:55.90 45.3psi after pressurize (stable) @04:46:55.94 Archive.in :compress @04:46:56.08 Archive.seek :empty @04:47:07.02 Reached 50.3 of 50.0psi after pumping 5108uL (2.95 strokes) @04:47:07.04 Archive.in :default @04:47:07.18 Archive.hold @04:47:07.30 Handoff.select :waste @04:47:19.58 delay 1:00 @04:48:19.62 Handoff.select :blocked @04:48:42.42 Handoff.select :waste @04:48:42.61 0.2psi at evacEnd @04:48:42.62 Handoff.select :blocked @04:48:42.81 Archive.in :fast @04:48:42.93 Archive.seek :topVent @04:48:56.37 Stable @0.3psi after Handoff blocked @04:48:56.43 Archive syringe pumping up to 8 strokes to reach 30psi @04:48:56.46 Archive stroke #1 of 8 @04:48:56.66 Archive.in :pressurize @04:48:56.78 Archive.seek :pressurized @04:49:09.92 0.6psi after pressurize @04:49:09.95 Archive.in :compress @04:49:10.09 Archive.seek :empty @04:49:24.76 14.4psi after compress @04:49:35.40 15.4psi after compress (stable) @04:49:35.48 Archive.in :gulp @04:49:35.62 Archive.seek :topVent @04:49:41.92 15.6psi after gulp @04:49:47.62 15.5psi after gulp (stable) @04:49:47.66 Archive stroke #2 of 8 @04:49:47.84 Archive.in :pressurize @04:49:47.94 Archive.seek :pressurized @04:50:00.48 15.4psi after pressurize @04:50:00.52 Archive.in :compress @04:50:00.66 Archive.seek :empty @04:50:15.33 28.5psi after compress @04:50:25.93 29.5psi after compress (stable) @04:50:25.99 Archive.in :gulp @04:50:26.10 Archive.seek :topVent @04:50:32.39 29.7psi after gulp @04:50:38.00 29.7psi after gulp (stable) @04:50:38.02 Archive stroke #3 of 8 @04:50:38.18 Archive.in :pressurize @04:50:38.28 Archive.seek :pressurized @04:50:50.82 29.5psi after pressurize @04:50:50.89 Archive.in :compress @04:50:51.04 Archive.seek :empty @04:50:56.10 Reached 30.5 of 30.3psi after pumping 4981uL (2.88 strokes) @04:50:56.13 Archive.in :default @04:50:56.27 Archive.hold @04:50:56.40 Handoff.select :waste @04:51:09.24 delay 1:00 @04:52:09.28 Handoff.select :blocked @04:52:32.03 Handoff.select :waste @04:52:32.21 0.3psi at evacEnd @04:52:32.27 Air.seek :fullVent @04:52:33.75 Archive.in :fast @04:52:33.86 Archive.seek :topVent @04:52:40.26 Seawater evacuated, Pressure above/below filter released. @04:52:40.40 Lysis1.in :slow @04:52:40.51 Lysis1.seek 1050uL @04:53:10.57 delay 5 seconds @04:53:15.59 Handoff.select :blocked @04:53:15.87 Lysis1.seek 450uL @04:54:15.85 delay 3 seconds @04:54:18.95 Air.in mix with 1.40048 parts Lysis1 @04:54:19.76 Air.seek :evac @04:54:19.81 Lysis1.seek 300uL @04:54:35.00 Lysis1.seek :empty @04:55:05.15 Lysis buffer delivered @24.2psi @04:55:05.17 Polling Can every 2:00 @04:55:05.74 PuckHeater.configure :default #ambient 24.9C @04:55:05.84 PuckHeater.seek 79.6C @04:55:54.41 PuckHeater.hold 2:00,79.6C @04:57:54.53 Polling Can every 2:00 @04:57:55.08 PuckHeater.configure :default #ambient 24.9C @04:57:55.18 PuckHeater.hold 30 seconds,78.6C @04:58:25.42 Polling Can every 2:00 @04:58:25.99 PuckHeater.configure :default #ambient 24.9C @04:58:26.14 PuckHeater.hold 30 seconds,78.1C @04:58:56.38 Polling Can every 2:00 @04:58:56.94 PuckHeater.configure :default #ambient 24.9C @04:58:57.04 PuckHeater.hold 10:00,77.6C @05:08:57.40 Lysis complete. Syringe pressure @28.7psi @05:08:57.43 Archive syringe pumping up to 5 strokes to reach 40psi @05:08:57.48 Archive stroke #1 of 5 @05:08:57.66 Archive.in :pressurize @05:08:57.79 Archive.seek :pressurized @05:09:10.37 28.0psi after pressurize @05:09:15.97 27.8psi after pressurize (stable) @05:09:16.00 Archive.in :compress @05:09:16.14 Archive.seek :empty @05:09:24.49 Reached 40.8 of 40.0psi after pumping 1593uL (0.92 strokes) @05:09:24.51 Archive.in :default @05:09:24.64 Archive.hold @05:09:24.75 delay 2 seconds @05:09:26.78 Archive.in :fast @05:09:26.88 Archive.seek :full @05:09:33.66 Lysis1.seek 138uL @05:09:47.63 delay 1 second @05:09:48.66 Archive.seek :full @05:09:48.77 Lysis1.seek :empty @05:10:02.78 Handoff.select :blocked @05:10:02.95 Initial air pressure @45.6psi @05:10:03.04 Lysis1.in :extract @05:10:03.27 Lysis1.seek 600uL,nil @05:10:06.63 Archive syringe pumping up to 6 strokes to reach 25psi @05:10:06.65 Archive stroke #1 of 6 @05:10:06.87 Archive.in :pressurize @05:10:07.08 Archive.seek :pressurized @05:10:19.73 11.1psi after pressurize @05:10:25.33 11.0psi after pressurize (stable) @05:10:25.36 Archive.in :compress @05:10:25.58 Archive.seek :empty @05:10:32.45 Archive.in :default @05:10:32.53 Archive.hold @05:10:32.81 Initial 600uL lysate @16.9psi @05:10:32.83 Handoff.select :blocked @05:10:33.02 Initial air pressure @17.6psi @05:10:33.13 Lysis1.seek 825uL,nil @05:10:35.24 Archive syringe pumping up to 6 strokes to reach 17psi @05:10:35.26 Archive stroke #1 of 6 @05:10:35.43 Archive.in :compress @05:10:35.56 Archive.seek :empty @05:10:35.79 Archive.in :default @05:10:35.87 Archive.hold @05:10:36.28 Recovered 825uL lysate @14.9psi @05:10:36.31 Air.in :halfSpeed @05:10:36.44 Air.seek :fullVent @05:10:38.74 Puck Top Vented, bottom @15.2psi @05:10:38.76 Handoff.select :waste @05:10:38.98 delay 5 seconds @05:10:44.11 Handoff open to waste, bottom @0.4psi @05:10:44.14 Air.in :default @05:10:44.31 Air.seek :evac @05:10:45.77 Lysis2.in :DAmix @05:10:46.00 Lysis1.in :DAmix @05:10:46.93 Lysis2.seek 700uL @05:10:46.99 Lysis1.seek 575uL @05:11:22.15 delay 5 seconds @05:11:27.17 Handoff.select SPR @05:11:27.36 SPR.command "start 2 14" @05:11:29.17 SPR stabilized @05:11:29.40 delay 10 seconds @05:11:39.42 SPR.command "start 2 0" @05:11:42.02 Waiting up to 11:00 for SPR to enter state 1 @05:13:39.69 SPR.command "hold_on" @05:13:41.08 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:13:41.82 Lysis2.seek 400uL @05:13:41.91 Lysis1.seek 275uL @05:14:23.97 delay 15 seconds @05:14:38.99 SPR.command "hold_off" @05:14:41.73 Waiting up to 38:00 for SPR to enter state 7 @05:29:01.91 SPRsummary:-4.63RIU,5.41RIU,98.4RIU,141RIU,high,109ng/L,171ng/L after 2858s @05:40:32.35 SPR analysis completed @05:40:39.94 Ended log spr23aug0704h.spr.bz2 @05:40:39.96 SPR.command "stats_off" @05:40:40.45 Selecting Cartridge 56 @05:40:40.68 Gate.power :heat,:OFF @05:40:40.70 Gate.power :cartridge,:OFF @05:40:40.85 PinPort.in :unlocking @05:40:40.96 PinPort.seek :clear @05:40:42.93 Carousel.in :default @05:40:43.10 Carousel.home.seek 57 @05:40:43.52 Archive.in :unlocking @05:40:43.76 Archive.seek :clear @05:40:43.83 Air.in :unlocking @05:40:44.11 Air.seek :clear @05:40:44.27 Lysis2.in :unlocking @05:40:44.44 Lysis2.seek :clear @05:40:48.35 Lysis1.in :unlocking @05:40:48.53 Lysis1.seek :clear @05:40:49.16 Plunge.in :unlocking @05:40:49.28 Plunge.seek :clear @05:40:52.44 Carousel.seek 56 @05:40:53.04 Lysis1.in :fast @05:40:53.17 Lysis1.seek :locked @05:40:55.85 Plunge.in :default @05:40:55.99 Plunge.seek :locked @05:40:56.16 Air.in :fast @05:40:56.27 Air.seek :locked @05:40:57.88 Carousel.reconfigure :locked @05:40:58.10 Powering cartridge bus @05:41:00.77 Gate.setIR 1750,5000,0,6 @05:41:00.91 Gate.setIR 0,5000,0,6 @05:41:01.03 Gate.power :cartridge,:OFF @05:41:01.62 Mounted archive Cartridge @05:41:02.03 Preparing to filter cartridge 56 @05:41:02.32 Gate.power :sampler,:ON @05:41:02.47 Priming sample loop w/150ml, bypass w/50ml @05:41:02.49 SamplePump.setPosition! 0ml @05:41:02.60 Bypass.open @05:41:04.12 Intake.open @05:41:04.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:41:07.51 Vacuum tare is 56% of full scale @05:41:07.75 Pressure.in :default @05:41:07.87 Pressure.hold @05:41:08.14 Waiting for external sampler to finish priming... @05:41:08.17 delayUntil "05:44:32.35" @05:44:32.40 SamplePump.in :prime @05:44:32.52 SamplePump.seek 50ml,2:53 @05:45:33.54 Pressure.stop @05:45:33.67 SamplePump.setPosition! 0ml @05:45:33.76 Exhaust.open @05:45:35.27 Bypass.close @05:45:36.86 Pressure.in :default @05:45:36.96 Pressure.hold @05:45:37.77 SamplePump.in :prime @05:45:37.98 SamplePump.seek 150ml,7:39 @05:48:30.44 Session Ended @05:48:40.17 Pressure.stop @05:48:40.33 Bypass.open @05:48:41.07 Intake.close @05:48:41.86 Toroid.in :hold @05:48:41.96 Toroid.seek :crack @05:48:43.52 Toroid.seek :cartridge @05:48:44.70 Toroid.in :default @05:48:44.79 Toroid.seek :clear @05:48:46.85 Pressurizing cartridge to 26psi above 14.8psia @05:48:46.87 SamplePump.setPosition! 0ml @05:48:46.95 Intake.open @05:48:47.63 Bypass.close @05:48:48.37 Exhaust.close @05:48:49.23 Pressure.in :default @05:48:49.33 Pressure.hold @05:48:49.62 SamplePump.in :charge @05:48:49.73 SamplePump.seek 2.3ml,42 seconds @05:48:54.71 Pressure.stop @05:48:54.82 Intake.close @05:48:55.62 Pressurized cartridge with 0.108ml to 26.1psi @05:48:55.64 Isolated @31.9psia (17.1psi) @05:48:55.66 Verifying cartridge holds at least 0.65..0.85 of 31.9psia @05:48:55.72 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @05:49:00.35 Cartridge pressure fell 6% to 30.0psia @05:49:00.48 Sampling 1000.0ml @05:49:00.50 SamplePump.setPosition! 0ml @05:49:00.60 Exhaust.open @05:49:01.35 Intake.open @05:49:02.21 Pressure.in :default @05:49:02.30 Pressure.hold @05:49:02.47 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @05:49:02.69 SamplePump.in :sample @05:49:02.79 SamplePump.seek 1000ml,1:23:51 @05:49:05.82 Cartridge 56 filtering 1/1000ml at 0.60ml/s with 22.1psi vacuum 3.5m deep (19.9psia) @05:50:05.82 Cartridge 56 filtering 36/1000ml at 0.53ml/s with 31.3psi vacuum @05:51:05.82 Cartridge 56 filtering 68/1000ml at 0.50ml/s with 15.2psi vacuum @05:52:05.83 Cartridge 56 filtering 100/1000ml at 0.52ml/s with 25.7psi vacuum @05:53:05.82 Cartridge 56 filtering 130/1000ml at 0.46ml/s with 20.8psi vacuum @05:54:05.82 Cartridge 56 filtering 160/1000ml at 0.49ml/s with 33.2psi vacuum @05:55:05.83 Cartridge 56 filtering 190/1000ml at 0.52ml/s with 23.6psi vacuum @05:56:05.82 Cartridge 56 filtering 219/1000ml at 0.45ml/s with 30.6psi vacuum @05:57:05.82 Cartridge 56 filtering 249/1000ml at 0.48ml/s with 11.8psi vacuum 3.0m deep (19.1psia) @05:58:05.82 Cartridge 56 filtering 278/1000ml at 0.49ml/s with 16.3psi vacuum 4.4m deep (21.2psia) @05:59:05.81 Cartridge 56 filtering 307/1000ml at 0.50ml/s with 24.0psi vacuum @06:00:05.82 Cartridge 56 filtering 336/1000ml at 0.50ml/s with 25.6psi vacuum @06:01:05.83 Cartridge 56 filtering 364/1000ml at 0.44ml/s with 24.7psi vacuum @06:02:05.83 Cartridge 56 filtering 393/1000ml at 0.47ml/s with 31.0psi vacuum @06:03:05.82 Cartridge 56 filtering 421/1000ml at 0.46ml/s with 31.1psi vacuum @06:04:05.82 Cartridge 56 filtering 450/1000ml at 0.46ml/s with 18.9psi vacuum @06:05:05.82 Cartridge 56 filtering 478/1000ml at 0.50ml/s with 28.1psi vacuum @06:06:05.83 Cartridge 56 filtering 506/1000ml at 0.47ml/s with 14.8psi vacuum 4.6m deep (21.4psia) @06:07:05.82 Cartridge 56 filtering 534/1000ml at 0.46ml/s with 29.2psi vacuum @06:08:05.82 Cartridge 56 filtering 562/1000ml at 0.48ml/s with 25.8psi vacuum @06:09:05.82 Cartridge 56 filtering 590/1000ml at 0.47ml/s with 29.3psi vacuum @06:10:05.82 Cartridge 56 filtering 618/1000ml at 0.47ml/s with 29.6psi vacuum @06:11:05.82 Cartridge 56 filtering 645/1000ml at 0.47ml/s with 25.4psi vacuum @06:11:12.80 Session Begun @06:11:16.90 -> showlog @06:12:05.82 Cartridge 56 filtering 672/1000ml at 0.45ml/s with 15.0psi vacuum 2.4m deep (18.2psia) @06:13:05.82 Cartridge 56 filtering 699/1000ml at 0.46ml/s with 21.6psi vacuum @06:14:05.81 Cartridge 56 filtering 726/1000ml at 0.44ml/s with 15.6psi vacuum @06:15:05.82 Cartridge 56 filtering 753/1000ml at 0.45ml/s with 15.2psi vacuum 3.3m deep (19.6psia) @06:16:05.81 Cartridge 56 filtering 780/1000ml at 0.47ml/s with 21.7psi vacuum 1.9m deep (17.5psia) @06:17:05.82 Cartridge 56 filtering 806/1000ml at 0.44ml/s with 15.5psi vacuum 2.7m deep (18.7psia) @06:18:05.81 Cartridge 56 filtering 832/1000ml at 0.44ml/s with 28.3psi vacuum @06:19:05.82 Cartridge 56 filtering 858/1000ml at 0.41ml/s with 14.8psi vacuum @06:20:05.83 Cartridge 56 filtering 884/1000ml at 0.44ml/s with 25.9psi vacuum @06:21:05.82 Cartridge 56 filtering 909/1000ml at 0.40ml/s with 19.7psi vacuum @06:22:05.83 Cartridge 56 filtering 934/1000ml at 0.43ml/s with 20.1psi vacuum 1.9m deep (17.5psia) @06:23:06.36 Cartridge 56 filtering 959/1000ml at 0.40ml/s with 29.3psi vacuum @06:24:05.83 Cartridge 56 filtering 983/1000ml at 0.40ml/s with 15.1psi vacuum @06:24:46.50 Pressure.stop @06:24:46.71 Intake.close @06:24:46.81 Exhaust.close @06:24:46.93 Handoff.select :close @06:24:47.20 Toroid.in :hold @06:24:47.32 Toroid.seek :bypass @06:24:48.99 Toroid.in :default @06:24:49.10 Toroid.seek :clear @06:24:50.77 Sampled 1000.0ml @06:24:51.50 Plunge.seek :reagent @06:24:52.38 Sample loop closed. Ready to Deliver Reagents @06:24:52.41 Archive.in :default @06:24:52.54 Archive.seek :sealed @06:24:53.94 Gate.power :sampler,:OFF @06:24:56.51 Archive.seek :evac @06:25:10.62 delay 1:00 @06:25:44.78 Session Begun @06:25:47.20 -> slot {[55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 56 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0704h.spr.bz2}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0700h.spr.bz2, was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0623h.spr.bz2, was: :dry}} @06:26:10.65 Archive.seek :clear @06:26:28.26 RNAlater soak for 10:00 @06:36:28.30 Archive.seek :tVent @06:36:30.81 delay 1 second @06:36:31.84 Archive.seek :evac @06:36:47.41 delay 10 seconds @06:36:57.45 Archive.seek :tVent @06:37:13.02 delay 1 second @06:37:14.05 Archive.seek :evac @06:37:29.63 delay 10 seconds @06:37:39.67 Sample preserved and evacuated @06:37:40.02 Carousel.in :default @06:37:40.22 Carousel.home.seek 56 @06:37:40.62 Plunge.seek :locked @06:37:41.58 Archive.in :unlocking @06:37:41.85 Archive.seek :clear @06:37:41.91 Air.in :unlocking @06:37:42.22 Air.seek :clear @06:37:42.30 Lysis1.in :unlocking @06:37:42.62 Lysis1.seek :clear @06:37:46.57 Gate.power :motor,:OFF @06:37:46.60 Gate.power :core,:OFF @06:37:46.66 Gate.power :analytic1,:OFF @06:37:46.74 Gate.power :analytic2,:OFF @06:37:46.78 Gate.power :main,:OFF @06:37:46.83 Safely stopped and ready to power off :STOPPED @06:38:06.87 -> slot {[55,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 56 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0704h.spr.bz2}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0700h.spr.bz2, was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0623h.spr.bz2, was: :dry}} @06:38:27.06 -> Cartridge.release 55 1 @06:39:11.21 -> fire({goalVolume:1000}, :microSPR_phyto) @06:39:11.33 Gate.power :sampler,:OFF,limit to 3500mA @06:39:11.35 Gate.power :main,:ON,limit to 5000mA @06:39:11.43 Gate.power :analytic1,:ON,limit to 5000mA @06:39:11.50 Gate.power :analytic2,:ON,limit to 5000mA @06:39:11.71 Powering core bus @06:39:14.02 SPR Module 1, FW Rev. B, FPGA Rev. C @06:39:14.50 Archive.configure :default @06:39:14.66 Air.configure :default @06:39:14.79 Lysis1.configure :default @06:39:14.92 Lysis2.configure :default @06:39:15.07 Plunge.configure :default @06:39:15.24 SamplePump.configure :default @06:39:15.40 Toroid.configure :default @06:39:15.56 Carousel.configure :default @06:39:15.76 Pressure.configure :default @06:39:15.97 ExtraPump.configure :default @06:39:16.16 PinPort.configure :default @06:39:16.31 All elf controllers running firmware version 4.84 @06:39:16.37 Gate.power :motor,:ON,limit to 5000mA @06:39:16.55 Archive.home.jog 0 @06:39:17.53 Carousel.home.jog 0 @06:39:17.63 Toroid.home.jog 0 @06:39:17.72 Lysis2.home.jog 0 @06:39:17.76 Lysis1.home.jog 0 @06:39:17.84 Plunge.home.jog 0 @06:39:17.92 PinPort.home.jog 0 @06:39:17.99 Air.home.jog 0 @06:39:19.02 Archive.in :homing @06:39:19.20 Archive.jog 1389 @06:39:19.49 PinPort.in :homing @06:39:19.56 Toroid.in :homing @06:39:19.67 Lysis2.in :homing @06:39:19.77 Air.in :homing @06:39:19.83 Plunge.in :homing @06:39:19.94 Lysis1.in :homing @06:39:20.33 PinPort.jog -107 @06:39:20.37 Toroid.jog 615 @06:39:20.47 Lysis2.jog 1390 @06:39:20.51 Air.jog 1358 @06:39:20.57 Plunge.jog 1572 @06:39:20.67 Lysis1.jog 1386 @06:39:21.17 PinPort.setRawPosition! 0 @06:39:21.21 Archive.setRawPosition! 50 @06:39:21.43 PinPort.seek :home @06:39:21.47 Archive.seek :home @06:39:22.41 Plunge.setRawPosition! 50 @06:39:22.47 Lysis2.setRawPosition! 50 @06:39:22.55 Air.setRawPosition! 50 @06:39:22.61 PinPort.in :default @06:39:22.66 Lysis1.setRawPosition! 50 @06:39:22.72 Archive.in :default @06:39:23.13 Plunge.seek :home @06:39:23.19 Lysis2.seek :home @06:39:23.24 Lysis1.seek :home @06:39:23.33 Air.seek :home @06:39:23.74 Plunge.in :default @06:39:23.78 Toroid.setRawPosition! 0 @06:39:24.02 Toroid.seek :home @06:39:24.25 Lysis1.in :default @06:39:24.28 Air.in :default @06:39:24.35 Lysis2.in :default @06:39:24.45 Toroid.in :default @06:39:24.99 Toroid.seek :clear @06:39:26.67 Carousel.in :default @06:39:26.88 Carousel.home.seek 56 @06:39:27.22 Selecting Cartridge 55 @06:39:27.65 Carousel.seek 55 @06:39:28.26 Lysis1.in :fast @06:39:28.39 Lysis1.seek :locked @06:39:31.08 Plunge.seek :locked @06:39:31.32 Air.in :fast @06:39:31.45 Air.seek :locked @06:39:33.07 Carousel.reconfigure :locked @06:39:33.32 Powering cartridge bus @06:39:35.99 Gate.setIR 1750,5000,0,6 @06:39:36.13 Gate.setIR 0,5000,0,6 @06:39:36.25 PuckHeater.configure :default #ambient 24.9C @06:39:36.39 Gate.power :heat,:ON,limit to 5000mA @06:39:37.11 Mounted lysis Cartridge board#Q05063 @06:39:37.20 Using default cartridge heat loss curve for HeatingAccelInCarousel @06:39:37.28 PhytoSampler with {goalVolume: 1000} @06:39:37.74 Preparing to filter cartridge 55 @06:39:38.06 Gate.power :sampler,:ON @06:39:38.21 Priming sample loop w/150ml, bypass w/50ml @06:39:38.23 SamplePump.setPosition! 0ml @06:39:38.37 Bypass.open @06:39:39.92 Intake.open @06:39:40.75 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:39:43.35 Vacuum tare is 56% of full scale @06:39:43.62 Pressure.in :default @06:39:43.76 Pressure.hold @06:39:44.06 Waiting for external sampler to finish priming... @06:39:44.08 delayUntil "06:43:08.08" @06:43:08.13 SamplePump.in :prime @06:43:08.27 SamplePump.seek 50ml,2:53 @06:44:09.31 Pressure.stop @06:44:09.43 SamplePump.setPosition! 0ml @06:44:09.53 Exhaust.open @06:44:11.02 Bypass.close @06:44:12.66 Pressure.in :default @06:44:12.80 Pressure.hold @06:44:13.18 SamplePump.in :prime @06:44:13.32 SamplePump.seek 150ml,7:39 @06:47:15.67 Pressure.stop @06:47:15.81 Bypass.open @06:47:16.60 Intake.close @06:47:17.46 Toroid.in :hold @06:47:17.62 Toroid.seek :crack @06:47:19.21 Toroid.seek :cartridge @06:47:20.38 Toroid.in :default @06:47:20.51 Toroid.seek :clear @06:47:22.59 Pressurizing cartridge to 26psi above 15.0psia @06:47:22.62 SamplePump.setPosition! 0ml @06:47:22.76 Intake.open @06:47:23.49 Bypass.close @06:47:24.25 Exhaust.close @06:47:25.14 Pressure.in :default @06:47:25.27 Pressure.hold @06:47:25.56 SamplePump.in :charge @06:47:25.73 SamplePump.seek 2.3ml,42 seconds @06:47:39.23 Pressure.stop @06:47:39.36 Intake.close @06:47:40.17 Pressurized cartridge with 0.818ml to 26.3psi @06:47:40.19 Isolated @34.7psia (19.7psi) @06:47:40.21 Verifying cartridge holds at least 0.65..0.85 of 34.7psia @06:47:40.35 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:47:45.05 Sampling 1000.0ml @06:47:45.07 SamplePump.setPosition! 0ml @06:47:45.20 Exhaust.open @06:47:45.95 Intake.open @06:47:46.83 Pressure.in :default @06:47:46.95 Pressure.hold @06:47:47.17 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @06:47:47.41 SamplePump.in :sample @06:47:47.55 SamplePump.seek 1000ml,1:23:51 @06:47:50.54 Cartridge 55 filtering 1/1000ml at 0.61ml/s with 26.1psi vacuum @06:48:50.55 Cartridge 55 filtering 38/1000ml at 0.61ml/s with 19.6psi vacuum 2.6m deep (18.5psia) @06:49:50.56 Cartridge 55 filtering 73/1000ml at 0.60ml/s with 18.0psi vacuum 3.5m deep (19.8psia) @06:50:50.56 Cartridge 55 filtering 109/1000ml at 0.56ml/s with 25.7psi vacuum @06:51:50.56 Cartridge 55 filtering 144/1000ml at 0.60ml/s with 25.9psi vacuum @06:52:50.54 Cartridge 55 filtering 179/1000ml at 0.58ml/s with 31.1psi vacuum @06:53:50.55 Cartridge 55 filtering 213/1000ml at 0.57ml/s with 26.2psi vacuum @06:54:50.55 Cartridge 55 filtering 247/1000ml at 0.54ml/s with 18.8psi vacuum @06:55:50.56 Cartridge 55 filtering 281/1000ml at 0.53ml/s with 24.1psi vacuum @06:56:50.55 Cartridge 55 filtering 315/1000ml at 0.57ml/s with 24.7psi vacuum @06:57:50.56 Cartridge 55 filtering 348/1000ml at 0.54ml/s with 13.8psi vacuum 2.1m deep (17.8psia) @06:58:50.56 Cartridge 55 filtering 381/1000ml at 0.56ml/s with 15.7psi vacuum 4.1m deep (20.8psia) @06:59:50.56 Cartridge 55 filtering 414/1000ml at 0.54ml/s with 33.3psi vacuum @07:00:50.55 Cartridge 55 filtering 447/1000ml at 0.52ml/s with 19.5psi vacuum @07:01:50.55 Cartridge 55 filtering 480/1000ml at 0.51ml/s with 23.1psi vacuum @07:02:50.55 Cartridge 55 filtering 513/1000ml at 0.55ml/s with 17.5psi vacuum 6.7m deep (24.6psia) @07:03:50.56 Cartridge 55 filtering 546/1000ml at 0.57ml/s with 23.0psi vacuum 1.9m deep (17.5psia) @07:04:50.55 Cartridge 55 filtering 579/1000ml at 0.54ml/s with 22.5psi vacuum 2.4m deep (18.3psia) @07:05:50.55 Cartridge 55 filtering 612/1000ml at 0.54ml/s with 16.8psi vacuum 6.9m deep (24.9psia) @07:06:50.56 Cartridge 55 filtering 644/1000ml at 0.54ml/s with 24.1psi vacuum @07:07:50.55 Cartridge 55 filtering 677/1000ml at 0.57ml/s with 22.9psi vacuum @07:08:50.99 Cartridge 55 filtering 709/1000ml at 0.51ml/s with 31.8psi vacuum @07:09:50.56 Cartridge 55 filtering 742/1000ml at 0.54ml/s with 15.2psi vacuum 3.1m deep (19.3psia) @07:10:50.55 Cartridge 55 filtering 774/1000ml at 0.57ml/s with 24.1psi vacuum @07:11:50.55 Cartridge 55 filtering 807/1000ml at 0.56ml/s with 25.8psi vacuum @07:12:50.55 Cartridge 55 filtering 839/1000ml at 0.56ml/s with 30.5psi vacuum @07:13:50.54 Cartridge 55 filtering 871/1000ml at 0.54ml/s with 28.6psi vacuum @07:14:50.55 Cartridge 55 filtering 904/1000ml at 0.56ml/s with 22.4psi vacuum @07:15:50.56 Cartridge 55 filtering 936/1000ml at 0.54ml/s with 14.4psi vacuum 4.0m deep (20.5psia) @07:16:50.56 Cartridge 55 filtering 968/1000ml at 0.54ml/s with 15.8psi vacuum @07:17:49.36 Pressure.stop @07:17:49.65 Intake.close @07:17:49.84 Exhaust.close @07:17:50.11 Toroid.in :hold @07:17:50.24 Toroid.seek :bypass @07:17:51.94 Toroid.in :default @07:17:52.07 Toroid.seek :clear @07:17:53.75 Sampled 1000.0ml @07:17:55.54 SPR.command "read_sequences" @07:17:55.82 SPR.command "stats_on" @07:17:56.05 SPR.command "start 0 1" @07:17:56.75 SPR.start /var/log/esp/spr23aug0707h.spr.bz2 @07:17:56.94 Gate.power :sampler,:OFF @07:17:58.68 Handoff.select :waste @07:18:05.48 Syringe tare is 18% of full scale @07:18:05.56 PinPort.in :locking @07:18:05.71 PinPort.seek :locked @07:18:08.33 Archive.in :fast @07:18:08.39 Lysis2.in :fast @07:18:08.52 Lysis1.seek 1350uL @07:18:08.82 Lysis2.seek 950uL @07:18:09.46 Archive.seek :full @07:18:14.30 Plunge.seek :reagent @07:18:15.34 Evacuating puck with filter bubble point of 50psi @07:18:15.38 PinPort.seek :locked @07:18:15.60 Handoff.select :waste @07:18:22.23 Air syringe pumping up to 1 strokes to reach 8psi @07:18:22.25 Air stroke #1 of 1 @07:18:22.44 Air.in :pressurize @07:18:22.56 Air.seek :pressurized @07:18:28.36 0.1psi after pressurize @07:18:28.43 Air.in :compress @07:18:28.58 Air.seek :evac @07:18:31.69 0.8psi after compress @07:18:44.80 0.4psi after compress (stable) @07:18:44.82 Air.in :default @07:18:44.99 Air.hold @07:18:45.28 at 0.3psi Opened to waste @07:18:45.30 Handoff.select :blocked @07:18:45.54 Archive syringe pumping up to 8 strokes to reach 50psi @07:18:45.56 Archive stroke #1 of 8 @07:18:45.81 Archive.in :pressurize @07:18:45.94 Archive.seek :pressurized @07:18:58.51 1.2psi after pressurize @07:19:04.13 1.2psi after pressurize (stable) @07:19:04.20 Archive.in :compress @07:19:04.33 Archive.seek :empty @07:19:19.04 14.6psi after compress @07:19:29.67 15.5psi after compress (stable) @07:19:29.74 Archive.in :gulp @07:19:29.87 Archive.seek :topVent @07:19:36.23 15.7psi after gulp @07:19:41.84 15.6psi after gulp (stable) @07:19:41.86 Archive stroke #2 of 8 @07:19:42.06 Archive.in :pressurize @07:19:42.17 Archive.seek :pressurized @07:19:54.75 15.4psi after pressurize @07:20:00.36 15.4psi after pressurize (stable) @07:20:00.39 Archive.in :compress @07:20:00.57 Archive.seek :empty @07:20:15.25 28.2psi after compress @07:20:25.87 29.5psi after compress (stable) @07:20:25.95 Archive.in :gulp @07:20:26.07 Archive.seek :topVent @07:20:32.40 29.7psi after gulp @07:20:38.02 29.7psi after gulp (stable) @07:20:38.04 Archive stroke #3 of 8 @07:20:38.27 Archive.in :pressurize @07:20:38.40 Archive.seek :pressurized @07:20:50.96 29.5psi after pressurize @07:20:57.10 29.4psi after pressurize (stable) @07:20:57.18 Archive.in :compress @07:20:57.29 Archive.seek :empty @07:21:11.98 41.8psi after compress @07:21:22.63 43.2psi after compress (stable) @07:21:22.71 Archive.in :gulp @07:21:22.83 Archive.seek :topVent @07:21:29.16 43.5psi after gulp @07:21:34.77 43.5psi after gulp (stable) @07:21:34.82 Archive stroke #4 of 8 @07:21:35.04 Archive.in :pressurize @07:21:35.16 Archive.seek :pressurized @07:21:47.73 43.1psi after pressurize @07:21:53.34 43.1psi after pressurize (stable) @07:21:53.46 Archive.in :compress @07:21:53.62 Archive.seek :empty @07:22:05.20 Reached 50.2 of 50.0psi after pumping 6851uL (3.96 strokes) @07:22:05.23 Archive.in :default @07:22:05.39 Archive.hold @07:22:05.53 Handoff.select :waste @07:22:18.81 delay 1:00 @07:23:18.85 Handoff.select :blocked @07:23:41.68 Handoff.select :waste @07:23:41.86 0.6psi at evacEnd @07:23:41.88 Handoff.select :blocked @07:23:42.10 Archive.in :fast @07:23:42.21 Archive.seek :topVent @07:23:55.74 Stable @0.7psi after Handoff blocked @07:23:55.83 Archive syringe pumping up to 8 strokes to reach 31psi @07:23:55.87 Archive stroke #1 of 8 @07:23:56.60 Archive.in :pressurize @07:23:56.74 Archive.seek :pressurized @07:24:09.32 0.7psi after pressurize @07:24:09.36 Archive.in :compress @07:24:09.53 Archive.seek :empty @07:24:24.24 11.5psi after compress @07:24:34.84 12.6psi after compress (stable) @07:24:34.92 Archive.in :gulp @07:24:35.03 Archive.seek :topVent @07:24:41.33 12.8psi after gulp @07:24:46.93 12.7psi after gulp (stable) @07:24:46.95 Archive stroke #2 of 8 @07:24:47.13 Archive.in :pressurize @07:24:47.26 Archive.seek :pressurized @07:24:59.82 12.7psi after pressurize @07:24:59.89 Archive.in :compress @07:25:00.01 Archive.seek :empty @07:25:14.72 23.2psi after compress @07:25:25.32 24.2psi after compress (stable) @07:25:25.36 Archive.in :gulp @07:25:25.51 Archive.seek :topVent @07:25:32.39 24.4psi after gulp @07:25:38.00 24.3psi after gulp (stable) @07:25:38.01 Archive stroke #3 of 8 @07:25:38.21 Archive.in :pressurize @07:25:38.33 Archive.seek :pressurized @07:25:50.88 24.3psi after pressurize @07:25:50.92 Archive.in :compress @07:25:51.08 Archive.seek :empty @07:26:01.89 Reached 30.8 of 30.7psi after pumping 5105uL (2.95 strokes) @07:26:01.91 Archive.in :default @07:26:02.13 Archive.hold @07:26:02.33 Handoff.select :waste @07:26:20.60 delay 1:00 @07:27:20.62 Handoff.select :blocked @07:27:43.38 Handoff.select :waste @07:27:43.56 1.4psi at evacEnd @07:27:43.63 Air.seek :fullVent @07:27:45.19 Archive.in :fast @07:27:45.32 Archive.seek :topVent @07:27:52.17 Seawater evacuated, Pressure above/below filter released. @07:27:52.37 Lysis1.in :slow @07:27:52.49 Lysis1.seek 1050uL @07:28:22.55 delay 5 seconds @07:28:27.58 Handoff.select :blocked @07:28:27.87 Lysis1.seek 450uL @07:29:27.87 delay 3 seconds @07:29:30.98 Air.in mix with 1.40048 parts Lysis1 @07:29:31.78 Air.seek :evac @07:29:31.87 Lysis1.seek 300uL @07:29:47.06 Lysis1.seek :empty @07:30:17.21 Lysis buffer delivered @4.0psi @07:30:17.23 Polling Can every 2:00 @07:30:17.90 PuckHeater.configure :default #ambient 25.6C @07:30:18.02 PuckHeater.seek 79.6C @07:31:01.40 PuckHeater.hold 2:00,79.6C @07:33:01.06 Polling Can every 2:00 @07:33:01.10 Errno::EPIPE in bill#2's_reader -- Broken pipe @07:33:01.29 Session Ended @07:33:07.23 Thread::Aborted in SPRlogger -- "Aborted by bill#2" @07:33:07.31 Thread::ChildDied in PROCESSING -- :SPRlogger while reading Puck Heater's status @07:33:07.34 Waiting to Resume from Checkpoint "Archive Syringe (ArS) pressureOutOfBounds at 85uL (sensed 99uL)" @07:33:11.81 Session Ended @07:55:53.35 Session Begun @07:55:56.19 -> showlog @07:56:57.39 -> Resume @07:56:57.45 NameError in bill3 -- uninitialized constant Resume @07:57:13.47 -> bill.resume @07:57:13.50 NoMethodError in bill3 -- private method `resume' called for :bill:Symbol @07:57:24.94 -> trouble >>> Thread[:PROCESSING] is suspended <<< @07:18:15 Evacuating puck with filter bubble point of 50psi @07:18:22 Air syringe pumping up to 1 strokes to reach 8psi @07:18:45 Archive syringe pumping up to 8 strokes to reach 50psi @07:22:04 Archive Syringe (ArS) pressureOutOfBounds at 69uL (sensed 84uL) @07:23:55 Archive syringe pumping up to 8 strokes to reach 31psi @07:26:01 Archive Syringe (ArS) pressureOutOfBounds at 85uL (sensed 99uL) @07:58:00.14 -> MainThread.exit # @07:58:00.36 ThreadError in bill3 -- killed thread @07:58:00.45 Session Ended @07:58:00.51 Session Ended @07:58:17.06PDT07-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @07:58:18.71 >BREAK< ESP3 Gateway boot v1.43 for core bus @07:58:18.73 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @07:58:22.17 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @07:58:22.77 Sea pressure (Abs) tare is 6% of full scale @07:58:32.80 Polling Can every 2:00 @07:58:32.85 Waiting for espclients... @07:58:40.96 Session Begun @07:58:42.80 -> slot {[48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [54,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 56 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @07:59:36.62 -> Cartridge.release 54 1 @08:01:24.18 -> fire :dry @08:01:24.38 Gate.power :sampler,:OFF,limit to 3500mA @08:01:24.42 Gate.power :analytic1,:ON,limit to 5000mA @08:01:24.44 Gate.power :analytic2,:ON,limit to 5000mA @08:01:25.17 Archive.configure :default @08:01:25.27 Archive.hold @08:01:25.38 Archive.configure :default @08:01:25.58 Air.configure :default @08:01:25.71 Air.hold @08:01:25.82 Air.configure :default @08:01:25.99 Lysis1.configure :default @08:01:26.08 Lysis1.hold @08:01:26.19 Lysis1.configure :default @08:01:26.36 Lysis2.configure :default @08:01:26.71 Lysis2.hold @08:01:26.84 Lysis2.configure :default @08:01:27.05 Plunge.configure :default @08:01:27.14 Plunge.hold @08:01:27.25 Plunge.configure :default @08:01:27.44 SamplePump.configure :default @08:01:27.66 Toroid.configure :default @08:01:27.78 Toroid.hold @08:01:28.63 Toroid.configure :default @08:01:28.85 Carousel.configure :default @08:01:28.95 Carousel.hold @08:01:29.06 Carousel.configure :default @08:01:29.27 Pressure.configure :default @08:01:29.46 ExtraPump.configure :default @08:01:29.69 PinPort.configure :default @08:01:29.78 PinPort.hold @08:01:29.89 PinPort.configure :default @08:01:29.98 All elf controllers running firmware version 4.84 @08:01:30.06 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:01:30.10 AnalyticModule::Unknown in bill -- SPR @08:01:30.88 Toroid.seek :clear @08:01:31.00 Carousel.in :default @08:01:31.16 Carousel.home.seek 55 @08:01:31.66 Carousel.reconfigure :locked @08:01:31.93 Gate.setIR 1750,5000,0,6 @08:01:32.06 Gate.setIR 0,5000,0,6 @08:01:32.16 PuckHeater.configure :default @08:01:32.24 Gate.power :heat,:ON,limit to 5000mA @08:01:33.03 Mounted lysis Cartridge board#Q05063 @08:01:33.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:01:33.16 Selecting Cartridge 54 @08:01:33.36 Gate.power :heat,:OFF @08:01:33.40 Gate.power :cartridge,:OFF @08:01:33.52 PinPort.in :unlocking @08:01:33.65 PinPort.seek :clear @08:01:35.63 Carousel.in :default @08:01:35.79 Carousel.home.seek 55 @08:01:36.17 Archive.in :unlocking @08:01:36.38 Archive.seek :clear @08:01:36.49 Air.in :unlocking @08:01:36.71 Air.seek :clear @08:01:36.77 Lysis2.in :unlocking @08:01:37.05 Lysis2.seek :clear @08:01:41.05 Lysis1.in :unlocking @08:01:41.24 Lysis1.seek :clear @08:01:41.32 Plunge.in :unlocking @08:01:41.46 Plunge.seek :clear @08:01:45.77 Carousel.seek 54 @08:01:46.34 Lysis1.in :fast @08:01:46.43 Lysis1.seek :locked @08:01:49.19 Plunge.in :default @08:01:49.33 Plunge.seek :locked @08:01:49.48 Air.in :fast @08:01:49.58 Air.seek :locked @08:01:51.21 Carousel.reconfigure :locked @08:01:51.40 Powering cartridge bus @08:01:54.07 Gate.setIR 1750,5000,0,6 @08:01:54.22 Gate.setIR 0,5000,0,6 @08:01:54.32 Gate.power :cartridge,:OFF @08:01:54.89 Mounted archive Cartridge @08:01:55.27 Preparing to filter cartridge 54 @08:01:55.55 Gate.power :sampler,:ON @08:01:55.71 Priming sample loop w/150ml, bypass w/50ml @08:01:55.73 SamplePump.setPosition! 0ml @08:01:55.81 Exhaust.close @08:01:55.93 Bypass.open @08:01:57.45 Intake.open @08:01:58.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:02:00.82 Vacuum tare is 56% of full scale @08:02:01.00 Pressure.in :default @08:02:01.08 Pressure.hold @08:02:01.33 Waiting for external sampler to finish priming... @08:02:01.35 delayUntil "08:05:25.58" @08:03:02.83 Session Begun @08:03:08.07 -> showlog @08:05:25.65 SamplePump.in :prime @08:05:25.77 SamplePump.seek 50ml,2:53 @08:06:26.76 Pressure.stop @08:06:26.86 SamplePump.setPosition! 0ml @08:06:26.95 Exhaust.open @08:06:28.47 Bypass.close @08:06:30.06 Pressure.in :default @08:06:30.16 Pressure.hold @08:06:30.52 SamplePump.in :prime @08:06:30.63 SamplePump.seek 150ml,7:39 @08:09:32.65 Pressure.stop @08:09:32.77 Bypass.open @08:09:33.54 Intake.close @08:09:34.41 Toroid.in :hold @08:09:34.56 Toroid.seek :crack @08:09:36.14 Toroid.seek :cartridge @08:09:37.30 Toroid.in :default @08:09:37.41 Toroid.seek :clear @08:09:40.02 Pressurizing cartridge to 26psi above 15.0psia @08:09:40.04 SamplePump.setPosition! 0ml @08:09:40.14 Intake.open @08:09:40.84 Bypass.close @08:09:41.63 Exhaust.close @08:09:42.52 Pressure.in :default @08:09:42.63 Pressure.hold @08:09:42.91 SamplePump.in :charge @08:09:43.03 SamplePump.seek 2.3ml,42 seconds @08:09:47.26 Pressure.stop @08:09:47.37 Intake.close @08:09:48.17 Pressurized cartridge with 0.076ml to 26.1psi @08:09:48.19 Isolated @33.6psia (18.6psi) @08:09:48.21 Verifying cartridge holds at least 0.65..0.85 of 33.6psia @08:09:48.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:09:52.93 Cartridge pressure fell 2% to 32.9psia @08:09:53.07 Sampling 1000.0ml @08:09:53.09 SamplePump.setPosition! 0ml @08:09:53.19 Exhaust.open @08:09:53.98 Intake.open @08:09:54.83 Pressure.hold @08:09:55.03 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:09:55.27 SamplePump.in :sample @08:09:55.40 SamplePump.seek 1000ml,1:23:51 @08:09:58.40 Cartridge 54 filtering 1/1000ml at 0.58ml/s with 21.1psi vacuum 2.9m deep (18.9psia) @08:10:58.41 Cartridge 54 filtering 34/1000ml at 0.52ml/s with 19.0psi vacuum @08:11:58.41 Cartridge 54 filtering 67/1000ml at 0.53ml/s with 32.5psi vacuum @08:12:58.41 Cartridge 54 filtering 100/1000ml at 0.51ml/s with 28.7psi vacuum @08:13:58.41 Cartridge 54 filtering 132/1000ml at 0.56ml/s with 27.5psi vacuum @08:14:58.41 Cartridge 54 filtering 165/1000ml at 0.54ml/s with 12.7psi vacuum 6.3m deep (24.0psia) @08:15:58.40 Cartridge 54 filtering 197/1000ml at 0.53ml/s with 29.0psi vacuum @08:16:58.42 Cartridge 54 filtering 229/1000ml at 0.52ml/s with 26.2psi vacuum @08:17:58.41 Cartridge 54 filtering 261/1000ml at 0.53ml/s with 29.0psi vacuum @08:18:58.41 Cartridge 54 filtering 293/1000ml at 0.56ml/s with 28.3psi vacuum @08:19:58.41 Cartridge 54 filtering 325/1000ml at 0.53ml/s with 10.3psi vacuum 4.3m deep (21.1psia) @08:20:58.40 Cartridge 54 filtering 357/1000ml at 0.55ml/s with 31.2psi vacuum @08:21:58.92 Cartridge 54 filtering 388/1000ml at 0.55ml/s with 28.6psi vacuum @08:22:58.41 Cartridge 54 filtering 420/1000ml at 0.55ml/s with 19.1psi vacuum 5.1m deep (22.3psia) @08:23:58.41 Cartridge 54 filtering 452/1000ml at 0.51ml/s with 10.4psi vacuum 1.7m deep (17.2psia) @08:24:58.41 Cartridge 54 filtering 483/1000ml at 0.54ml/s with 19.2psi vacuum 6.6m deep (24.4psia) @08:25:58.41 Cartridge 54 filtering 515/1000ml at 0.51ml/s with 11.8psi vacuum @08:26:58.41 Cartridge 54 filtering 546/1000ml at 0.52ml/s with 9.7psi vacuum 3.6m deep (20.0psia) @08:27:58.41 Cartridge 54 filtering 578/1000ml at 0.55ml/s with 23.6psi vacuum @08:28:58.41 Cartridge 54 filtering 609/1000ml at 0.55ml/s with 26.2psi vacuum @08:29:58.41 Cartridge 54 filtering 641/1000ml at 0.55ml/s with 23.8psi vacuum @08:30:58.41 Cartridge 54 filtering 672/1000ml at 0.52ml/s with 9.9psi vacuum 3.6m deep (20.1psia) @08:31:58.41 Cartridge 54 filtering 703/1000ml at 0.55ml/s with 24.1psi vacuum @08:32:58.41 Cartridge 54 filtering 735/1000ml at 0.55ml/s with 25.6psi vacuum @08:33:58.41 Cartridge 54 filtering 766/1000ml at 0.54ml/s with 17.3psi vacuum 3.6m deep (20.1psia) @08:34:58.41 Cartridge 54 filtering 798/1000ml at 0.50ml/s with 12.8psi vacuum @08:35:58.41 Cartridge 54 filtering 829/1000ml at 0.54ml/s with 22.5psi vacuum 3.4m deep (19.7psia) @08:36:58.41 Cartridge 54 filtering 860/1000ml at 0.55ml/s with 26.1psi vacuum @08:37:58.41 Cartridge 54 filtering 892/1000ml at 0.54ml/s with 24.7psi vacuum @08:38:58.41 Cartridge 54 filtering 923/1000ml at 0.48ml/s with 21.2psi vacuum @08:39:58.40 Cartridge 54 filtering 954/1000ml at 0.52ml/s with 10.1psi vacuum 4.4m deep (21.3psia) @08:40:58.41 Cartridge 54 filtering 985/1000ml at 0.55ml/s with 23.8psi vacuum @08:41:26.77 Pressure.stop @08:41:26.97 Intake.close @08:41:27.02 Exhaust.close @08:41:27.33 Toroid.in :hold @08:41:27.44 Toroid.seek :bypass @08:41:29.13 Toroid.in :default @08:41:29.24 Toroid.seek :clear @08:41:30.90 Sampled 1000.0ml @08:41:31.66 Plunge.seek :reagent @08:41:32.49 Sample loop closed. Ready to Deliver Reagents @08:41:32.52 Archive.in :default @08:41:32.65 Archive.seek :sealed @08:41:34.07 Gate.power :sampler,:OFF @08:41:36.61 Archive.seek :evac @08:41:50.71 delay 1:00 @08:42:50.74 Archive.seek :clear @08:43:08.27 RNAlater soak for 10:00 @08:45:01.73 -> slot {[48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 56 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @08:45:35.97 -> Cartridge.release 49..6 44 @08:52:00.52 -> showlog @08:53:08.31 Archive.seek :tVent @08:53:10.81 delay 1 second @08:53:11.83 Archive.seek :evac @08:53:27.38 delay 10 seconds @08:53:37.41 Archive.seek :tVent @08:53:52.97 delay 1 second @08:53:53.99 Archive.seek :evac @08:54:09.55 delay 10 seconds @08:54:19.60 Sample preserved and evacuated @08:54:19.94 Carousel.in :default @08:54:20.11 Carousel.home.seek 54 @08:54:20.50 Plunge.seek :locked @08:54:21.48 Archive.in :unlocking @08:54:21.70 Archive.seek :clear @08:54:21.87 Air.in :unlocking @08:54:22.06 Air.seek :clear @08:54:22.22 Lysis1.in :unlocking @08:54:22.44 Lysis1.seek :clear @08:54:26.31 Gate.power :motor,:OFF @08:54:26.33 Gate.power :core,:OFF @08:54:26.38 Gate.power :analytic1,:OFF @08:54:26.42 Gate.power :analytic2,:OFF @08:54:26.49 Gate.power :main,:OFF @08:54:26.52 Safely stopped and ready to power off :STOPPED @08:57:04.91 Session Ended @08:57:24.79 Session Begun @08:57:28.09 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 56 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @08:58:09.24 -> exit @08:58:09.27 Session Ended @08:58:22.87 Session Begun @08:58:29.02 -> ESP.shutdow @08:58:29.05 NoMethodError in bill -- undefined method `shutdow' for ESP:Module @08:58:34.92 -> ESP.shutdown [] @08:58:43.54 -> MainThread.exit # @08:58:43.70 ThreadError in bill -- killed thread @08:58:43.80 Session Ended @08:58:43.87 Session Ended @11:08:02.13PDT07-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @11:08:06.04 >BREAK< ESP3 Gateway boot v1.43 for core bus @11:08:06.07 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @11:08:10.30 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @11:08:11.42 Sea pressure (Abs) tare is 6% of full scale @11:08:21.34 Polling Can every 2:00 @11:08:21.44 Waiting for espclients... @11:08:21.58 Session Begun @11:08:31.99 -> start esp @11:08:32.04 LocalJumpError in esp -- no block given # @11:08:39.30 -> ESP.ready! @11:08:39.36 Gate.power :sampler,:OFF,limit to 3500mA @11:08:39.40 Gate.power :main,:ON,limit to 5000mA @11:08:39.42 Gate.power :analytic1,:ON,limit to 5000mA @11:08:39.46 Gate.power :analytic2,:ON,limit to 5000mA @11:08:39.69 Powering core bus @11:08:41.92 SPR Module 1, FW Rev. B, FPGA Rev. C @11:08:42.44 Archive.configure :default @11:08:42.56 Air.configure :default @11:08:42.65 Lysis1.configure :default @11:08:42.73 Lysis2.configure :default @11:08:42.85 Plunge.configure :default @11:08:42.97 SamplePump.configure :default @11:08:43.10 Toroid.configure :default @11:08:43.21 Carousel.configure :default @11:08:43.34 Pressure.configure :default @11:08:43.45 ExtraPump.configure :default @11:08:43.57 PinPort.configure :default @11:08:43.66 All elf controllers running firmware version 4.84 @11:08:43.70 Gate.power :motor,:ON,limit to 5000mA @11:08:43.92 Archive.home.jog 0 @11:08:44.75 Carousel.home.jog 0 @11:08:44.80 Toroid.home.jog 0 @11:08:44.84 Lysis2.home.jog 0 @11:08:44.88 Lysis1.home.jog 0 @11:08:44.92 Plunge.home.jog 0 @11:08:44.96 Air.home.jog 0 @11:08:45.00 PinPort.home.jog 0 @11:08:46.39 Archive.in :homing @11:08:46.64 Archive.jog 1382 @11:08:46.72 Toroid.in :homing @11:08:46.77 Lysis2.in :homing @11:08:46.81 Air.in :homing @11:08:46.87 PinPort.in :homing @11:08:46.94 Lysis1.in :homing @11:08:46.99 Plunge.in :homing @11:08:47.35 Toroid.jog 615 @11:08:47.41 PinPort.jog -107 @11:08:47.46 Lysis2.jog 1387 @11:08:47.50 Air.jog 1357 @11:08:47.57 Lysis1.jog 1385 @11:08:47.65 Plunge.jog 1565 @11:08:47.99 PinPort.setRawPosition! 0 @11:08:48.06 PinPort.seek :home @11:08:48.18 PinPort.in :default @11:08:48.30 Archive.setRawPosition! 50 @11:08:48.37 Archive.seek :home @11:08:48.67 Archive.in :default @11:08:48.93 Lysis2.setRawPosition! 50 @11:08:49.01 Lysis2.seek :home @11:08:49.16 Lysis1.setRawPosition! 50 @11:08:49.19 Air.setRawPosition! 50 @11:08:49.23 Plunge.setRawPosition! 50 @11:08:49.41 Lysis1.seek :home @11:08:49.46 Air.seek :home @11:08:49.58 Plunge.seek :home @11:08:50.01 Lysis1.in :default @11:08:50.06 Air.in :default @11:08:50.16 Toroid.setRawPosition! 0 @11:08:50.22 Lysis2.in :default @11:08:50.45 Toroid.seek :home @11:08:50.59 Plunge.in :default @11:08:50.62 Toroid.in :default @11:08:51.02 Toroid.seek :clear @11:08:52.66 Carousel.in :default @11:08:52.82 Carousel.home.seek 54 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @11:09:02.94 -> ESP.ready1 @11:09:02.98 NoMethodError in n -- undefined method `ready1' for ESP:Module @11:09:09.49 -> ESP.reready! @11:09:09.56 Archive.in :default @11:09:09.65 Air.in :default @11:09:09.71 Lysis1.in :default @11:09:09.83 Lysis2.in :default @11:09:09.98 Plunge.in :default @11:09:10.13 Archive.home.jog 0 @11:09:10.19 Toroid.in :default @11:09:10.27 Carousel.in :default @11:09:10.33 Air.home.jog 0 @11:09:10.38 PinPort.in :default @11:09:10.47 Lysis1.home.jog 0 @11:09:10.58 Lysis2.home.jog 0 @11:09:10.79 Plunge.home.jog 0 @11:09:10.90 Toroid.home.jog 0 @11:09:11.03 PinPort.home.jog 0 @11:09:11.08 Carousel.home.jog 0 @11:09:12.28 Archive.in :homing @11:09:12.63 Archive.jog 1388 @11:09:12.97 PinPort.in :homing @11:09:13.00 Toroid.in :homing @11:09:13.06 Lysis2.in :homing @11:09:13.11 Air.in :homing @11:09:13.18 Plunge.in :homing @11:09:13.23 Lysis1.in :homing @11:09:13.51 PinPort.jog -108 @11:09:13.66 Toroid.jog 609 @11:09:13.70 Lysis2.jog 1393 @11:09:13.76 Plunge.jog 407 @11:09:13.81 Lysis1.jog 1381 @11:09:13.87 Air.jog 1360 @11:09:14.32 PinPort.setRawPosition! 0 @11:09:14.35 Archive.setRawPosition! 50 @11:09:14.46 PinPort.seek :home @11:09:14.54 Archive.seek :home @11:09:14.80 Plunge.setRawPosition! 50 @11:09:14.83 PinPort.in :default @11:09:14.96 Plunge.seek :home @11:09:15.09 Archive.in :default @11:09:15.24 Lysis2.setRawPosition! 50 @11:09:15.38 Plunge.in :default @11:09:15.41 Lysis1.setRawPosition! 50 @11:09:15.45 Air.setRawPosition! 50 @11:09:15.49 Lysis2.seek :home @11:09:15.74 Lysis1.seek :home @11:09:15.80 Air.seek :home @11:09:16.03 Lysis2.in :default @11:09:16.18 Toroid.setRawPosition! 0 @11:09:16.22 Lysis1.in :default @11:09:16.33 Toroid.seek :home @11:09:16.48 Air.in :default @11:09:16.60 Toroid.in :default @11:09:16.86 Toroid.seek :clear @11:09:18.49 Carousel.in :default @11:09:18.65 Carousel.home.seek 54 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @11:09:24.13 -> Sampler.psi 0.721052158287591 @11:09:28.58 -> Leak.psia 16.2025607024066 @11:09:31.52 -> Can Can@11:08:21, 24.9C, 43% humidity, 14.3psia, 14.880V, 0.085A, 0.003Ah, 1.26W @11:10:16.72 -> PB.close;Exhaust.close;Intake.close Intake is closed @11:10:22.40 -> Power.sampler :ON @11:10:22.45 Gate.power :sampler,:ON true @11:10:27.52 -> Power.sampler :OFF @11:10:27.59 Gate.power :sampler,:OFF false @11:10:41.46 Session Begun @11:11:36.98 -> -> start esp @11:11:37.04 SyntaxError in n -- compile error n:11: syntax error, unexpected '>' -> start esp ^ @11:11:37.23 -> -> espclient nadia #replace Nadia with your name @11:11:37.26 SyntaxError in n -- compile error n:12: syntax error, unexpected '>' -> espclient nadia #replace Nadia with your name ^ @11:11:37.49 -> -> Power.sampler :ON #turn on external pump for at least 20 seconds @11:11:37.58 SyntaxError in n -- compile error n:13: syntax error, unexpected '>' -> Power.sampler :ON #turn ... ^ @11:11:37.71 -> -> require ‘vacuumCart’ @11:11:37.78 SyntaxError in n -- compile error n:14: syntax error, unexpected '>' -> require ‘vacuumCart’ ^ n:14: Invalid char `\342' in expression n:14: Invalid char `\200' in expression n:14: Invalid char `\230' in expression n:14: Invalid char `\342' in expression n:14: Invalid char `\200' in expression n:14: Invalid char `\231' in expression @11:11:38.19 -> -> 3.times {refillSampleLines} #primes the lines 3 times without monitoring pressure. You will likely get a leak error if it was monitoring pressure if there is air in the lines. @11:11:38.21 SyntaxError in n -- compile error n:15: syntax error, unexpected '>' -> 3.times {refillSampleLines} ... ^ @11:11:38.59 -> -> BACsampler.prime 150, 50 #primes the lines while monitoring pressure, 50mL for the bypass loop and 150mL for the sample loop @11:11:38.62 SyntaxError in n -- compile error n:16: syntax error, unexpected '>' -> BACsampler.prime 150, 50 ... ^ @11:11:38.83 -> -> Power.sampler :OFF #turns off the external pump @11:11:38.88 SyntaxError in n -- compile error n:17: syntax error, unexpected '>' -> Power.sampler :OFF #turns off the external pump ^ @11:11:39.05 -> -> Can #check Can temperature @11:11:39.12 SyntaxError in n -- compile error n:18: syntax error, unexpected '>' -> Can #check Can temperature ^ @11:11:39.32 -> -> slots #shows the status and cartridge type of each slot @11:11:39.37 SyntaxError in n -- compile error n:19: syntax error, unexpected '>' -> slots #shows the status and cartridge type of each slot ^ @11:11:39.61 -> When ready to fire, to fire an archive then a lyse n go after: @11:11:39.64 SyntaxError in n -- compile error n:20: syntax error, unexpected tIDENTIFIER, expecting kDO or '{' or '(' When ready to fire, to fire an archive then a lyse n go after: ^ n:20: syntax error, unexpected kTHEN, expecting $end When ready to fire, to fire an archive then a lyse n go after: ^ @11:15:43.65 -> exit @11:15:43.68 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:16:06.01 Session Begun @11:16:17.92 -> ESP.reready! @11:16:17.99 Archive.in :default @11:16:18.09 Air.in :default @11:16:18.20 Lysis1.in :default @11:16:18.32 Lysis2.in :default @11:16:18.46 Plunge.in :default @11:16:18.55 Toroid.in :default @11:16:18.72 Archive.home.jog 0 @11:16:18.78 Carousel.in :default @11:16:18.89 PinPort.in :default @11:16:19.12 Air.home.jog 0 @11:16:19.22 Lysis1.home.jog 0 @11:16:19.39 Lysis2.home.jog 0 @11:16:19.46 Plunge.home.jog 0 @11:16:20.09 Toroid.home.jog 0 @11:16:20.25 Carousel.home.jog 0 @11:16:20.33 PinPort.home.jog 0 @11:16:21.43 Archive.in :homing @11:16:21.73 Archive.jog 1386 @11:16:21.81 PinPort.in :homing @11:16:21.88 Plunge.in :homing @11:16:21.94 Lysis1.in :homing @11:16:22.04 Toroid.in :homing @11:16:22.11 Air.in :homing @11:16:22.17 Lysis2.in :homing @11:16:22.58 PinPort.jog -107 @11:16:22.62 Plunge.jog 402 @11:16:22.67 Lysis1.jog 1383 @11:16:22.79 Toroid.jog 610 @11:16:22.93 Air.jog 1360 @11:16:23.03 Lysis2.jog 1391 @11:16:23.45 PinPort.setRawPosition! 0 @11:16:23.58 PinPort.seek :home @11:16:23.78 PinPort.in :default @11:16:23.81 Plunge.setRawPosition! 50 @11:16:23.88 Archive.setRawPosition! 50 @11:16:24.06 Plunge.seek :home @11:16:24.20 Archive.seek :home @11:16:24.38 Lysis1.setRawPosition! 50 @11:16:24.50 Lysis1.seek :home @11:16:24.66 Lysis2.setRawPosition! 50 @11:16:24.70 Air.setRawPosition! 50 @11:16:24.76 Archive.in :default @11:16:24.83 Plunge.in :default @11:16:25.09 Air.seek :home @11:16:25.16 Lysis2.seek :home @11:16:25.43 Lysis1.in :default @11:16:25.46 Toroid.setRawPosition! 0 @11:16:25.70 Toroid.seek :home @11:16:25.90 Toroid.in :default @11:16:25.94 Lysis2.in :default @11:16:26.07 Air.in :default @11:16:26.51 Toroid.seek :clear @11:16:28.18 Carousel.in :default @11:16:28.35 Carousel.home.seek 54 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @11:17:14.16 -> ESP.ready! @11:17:15.02 Toroid.seek :clear @11:17:15.14 Carousel.in :default @11:17:15.31 Carousel.home.seek 54 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @11:17:22.49 -> ESP.reready! @11:17:22.53 Archive.in :default @11:17:22.70 Air.in :default @11:17:22.92 Lysis2.in :default @11:17:23.05 Lysis1.in :default @11:17:23.15 Plunge.in :default @11:17:23.27 Toroid.in :default @11:17:23.34 Carousel.in :default @11:17:23.44 Archive.home.jog 0 @11:17:23.57 PinPort.in :default @11:17:23.79 Air.home.jog 0 @11:17:23.94 Lysis2.home.jog 0 @11:17:24.09 Lysis1.home.jog 0 @11:17:24.32 Plunge.home.jog 0 @11:17:24.40 PinPort.home.jog 0 @11:17:24.55 Toroid.home.jog 0 @11:17:24.64 Carousel.home.jog 0 @11:17:25.47 Archive.in :homing @11:17:25.85 PinPort.in :homing @11:17:25.98 Lysis2.in :homing @11:17:26.06 Archive.jog 1386 @11:17:26.13 Toroid.in :homing @11:17:26.18 Air.in :homing @11:17:26.25 Lysis1.in :homing @11:17:26.33 Plunge.in :homing @11:17:26.70 PinPort.jog -108 @11:17:26.74 Lysis2.jog 1393 @11:17:26.90 Toroid.jog 610 @11:17:27.02 Air.jog 1362 @11:17:27.08 Plunge.jog 405 @11:17:27.18 Lysis1.jog 1386 @11:17:27.61 PinPort.setRawPosition! 0 @11:17:27.72 PinPort.seek :home @11:17:27.86 Archive.setRawPosition! 50 @11:17:27.90 PinPort.in :default @11:17:28.07 Archive.seek :home @11:17:28.23 Plunge.setRawPosition! 50 @11:17:28.31 Plunge.seek :home @11:17:28.47 Lysis2.setRawPosition! 50 @11:17:28.49 Archive.in :default @11:17:28.67 Lysis2.seek :home @11:17:28.86 Plunge.in :default @11:17:28.89 Lysis1.setRawPosition! 50 @11:17:28.93 Air.setRawPosition! 50 @11:17:29.16 Lysis1.seek :home @11:17:29.27 Air.seek :home @11:17:29.44 Toroid.setRawPosition! 0 @11:17:29.48 Lysis2.in :default @11:17:29.71 Toroid.seek :home @11:17:29.88 Lysis1.in :default @11:17:29.93 Toroid.in :default @11:17:29.99 Air.in :default @11:17:30.48 Toroid.seek :clear @11:17:32.14 Carousel.in :default @11:17:32.33 Carousel.home.seek 54 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @11:17:40.74 -> SPR.start @11:17:41.98 SPR.command "read_sequences" @11:17:42.78 SPR.command "stats_on" # @11:17:43.68 SPR.start /var/log/esp/spr23aug0711h.spr.bz2 @11:17:51.30 -> SPR.stop @11:17:51.40 Ended log spr23aug0711h.spr.bz2 @11:17:51.42 SPR.command "stats_off" @11:20:26.44 -> exit @11:20:26.49 Session Ended @11:20:38.07 Session Begun @11:20:42.05 -> ESP.shutdown @11:20:42.28 Gate.power :motor,:OFF @11:20:42.31 Gate.power :core,:OFF @11:20:42.36 Gate.power :analytic1,:OFF @11:20:42.41 Gate.power :analytic2,:OFF @11:20:42.44 Gate.power :main,:OFF [:motor,:core,:analytic1,:analytic2,:main] @11:20:49.20 -> exit @11:20:49.25 Session Ended @12:36:36.14PDT07-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @12:36:37.88 >BREAK< ESP3 Gateway boot v1.43 for core bus @12:36:37.91 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @12:36:41.53 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @12:36:42.18 Sea pressure (Abs) tare is 6% of full scale @12:36:51.16 Polling Can every 2:00 @12:36:51.22 Waiting for espclients... @12:38:02.36 Session Begun @12:38:13.74 -> Can Can@12:36:51, 23.0C, 42% humidity, 14.3psia, 14.887V, 0.082A, 0.003Ah, 1.22W @12:39:37.42 -> ESP.shutdown [] @12:39:57.88 -> exit @12:39:57.90 Session Ended @12:57:00.44 Session Begun @12:57:32.71 -> Can Can@12:56:47, 24.9C, 37% humidity, 14.3psia, 14.887V, 0.083A, 0.030Ah, 1.24W @12:58:02.87 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @12:59:17.12 -> exit @12:59:17.16 Session Ended @13:38:02.42 Session Begun @13:38:16.06 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @13:38:38.38 -> show log @13:38:38.42 NoMethodError in errera -- undefined method `show' for CmdServer::Client:Class @13:38:55.66 -> showlog @13:39:39.32 -> Can Can@13:38:39, 24.8C, 36% humidity, 14.3psia, 14.887V, 0.081A, 0.088Ah, 1.21W @13:49:48.83 Session Begun @13:50:10.96 -> ESP.ready! @13:50:11.03 Gate.power :sampler,:OFF,limit to 3500mA @13:50:11.06 Gate.power :main,:ON,limit to 5000mA @13:50:11.10 Gate.power :analytic1,:ON,limit to 5000mA @13:50:11.14 Gate.power :analytic2,:ON,limit to 5000mA @13:50:11.35 Powering core bus @13:50:13.59 SPR Module 1, FW Rev. B, FPGA Rev. C @13:50:14.12 Archive.configure :default @13:50:14.26 Air.configure :default @13:50:14.36 Lysis1.configure :default @13:50:14.47 Lysis2.configure :default @13:50:14.59 Plunge.configure :default @13:50:14.72 SamplePump.configure :default @13:50:14.85 Toroid.configure :default @13:50:14.99 Carousel.configure :default @13:50:15.12 Pressure.configure :default @13:50:15.75 ExtraPump.configure :default @13:50:15.86 PinPort.configure :default @13:50:15.96 All elf controllers running firmware version 4.84 @13:50:16.03 Gate.power :motor,:ON,limit to 5000mA @13:50:16.42 Archive.home.jog 0 @13:50:17.05 Plunge.home.jog 0 @13:50:17.20 Carousel.home.jog 0 @13:50:17.25 Lysis2.home.jog 0 @13:50:17.33 Air.home.jog 0 @13:50:17.38 Toroid.home.jog 0 @13:50:17.44 Lysis1.home.jog 0 @13:50:17.52 PinPort.home.jog 0 @13:50:18.45 Archive.in :homing @13:50:18.72 Archive.jog 1389 @13:50:18.80 Toroid.in :homing @13:50:18.85 Lysis2.in :homing @13:50:18.93 Air.in :homing @13:50:18.99 PinPort.in :homing @13:50:19.06 Plunge.in :homing @13:50:19.11 Lysis1.in :homing @13:50:19.55 Toroid.jog 614 @13:50:19.62 Lysis2.jog 1391 @13:50:19.70 Plunge.jog 402 @13:50:19.75 Air.jog 1364 @13:50:19.84 Lysis1.jog 1387 @13:50:19.91 PinPort.jog -107 @13:50:20.38 Archive.setRawPosition! 50 @13:50:20.46 Archive.seek :home @13:50:20.59 PinPort.setRawPosition! 0 @13:50:20.71 PinPort.seek :home @13:50:20.74 Plunge.setRawPosition! 50 @13:50:20.97 PinPort.in :default @13:50:21.01 Archive.in :default @13:50:21.05 Plunge.seek :home @13:50:21.36 Lysis2.setRawPosition! 50 @13:50:21.42 Lysis1.setRawPosition! 50 @13:50:21.48 Air.setRawPosition! 50 @13:50:21.70 Lysis2.seek :home @13:50:21.76 Lysis1.seek :home @13:50:21.88 Air.seek :home @13:50:22.12 Toroid.setRawPosition! 0 @13:50:22.16 Plunge.in :default @13:50:22.32 Toroid.seek :home @13:50:22.52 Lysis2.in :default @13:50:22.58 Air.in :default @13:50:22.65 Toroid.in :default @13:50:22.69 Lysis1.in :default @13:50:23.23 Toroid.seek :clear @13:50:24.89 Carousel.in :default @13:50:25.08 Carousel.home.seek 55 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Pressure = Absolute Pressure (Leak) Sensor @14psia PinPort = Handoff Pin Port (PP) clear @13:51:09.43 -> Power.sampler :ON @13:51:09.47 Gate.power :sampler,:ON true @13:51:27.03 -> require 'vacuumCart' true @13:52:27.33 -> 3.times{refillSampleLines} @13:52:27.38 Refilling sample & bypass lines with fluid. @13:52:28.81 Toroid.seek :clear @13:52:28.94 Carousel.in :default @13:52:29.11 Carousel.home.seek 55 @13:52:29.48 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:52:29.52 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:52:29.55 Bypass.open @13:52:30.36 Intake.open @13:52:31.07 SamplePump.setPosition! 0ml @13:52:31.23 SamplePump.seek 30ml @13:53:08.12 Bypass.close @13:53:08.83 Exhaust.open @13:53:09.70 SamplePump.seek 55ml @13:53:40.53 delay 1 second @13:53:41.57 Intake.close @13:53:41.67 Exhaust.close @13:53:42.46 Sampling Loop filled with water @13:53:42.49 Refilling sample & bypass lines with fluid. @13:53:43.37 Toroid.seek :clear @13:53:43.54 Carousel.in :default @13:53:43.71 Carousel.home.seek 55 @13:53:44.04 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:53:44.10 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:53:44.11 Bypass.open @13:53:44.87 Intake.open @13:53:45.57 SamplePump.setPosition! 0ml @13:53:45.74 SamplePump.seek 30ml @13:54:22.62 Bypass.close @13:54:23.39 Exhaust.open @13:54:24.25 SamplePump.seek 55ml @13:54:55.07 delay 1 second @13:54:56.11 Intake.close @13:54:56.21 Exhaust.close @13:54:56.99 Sampling Loop filled with water @13:54:57.01 Refilling sample & bypass lines with fluid. @13:54:57.91 Toroid.seek :clear @13:54:58.04 Carousel.in :default @13:54:58.22 Carousel.home.seek 55 @13:54:58.55 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:55:07.39 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:55:07.44 Bypass.open @13:55:08.22 Intake.open @13:55:08.92 SamplePump.setPosition! 0ml @13:55:09.09 SamplePump.seek 30ml @13:55:45.98 Bypass.close @13:55:46.72 Exhaust.open @13:55:47.54 SamplePump.seek 55ml @13:56:18.35 delay 1 second @13:56:19.38 Intake.close @13:56:20.04 Exhaust.close @13:56:20.89 Sampling Loop filled with water 3 @13:56:50.03 -> BACsampler.prime @13:56:50.16 Priming sample loop w/150ml, bypass w/50ml @13:56:50.19 SamplePump.setPosition! 0ml @13:56:50.29 Bypass.open @13:56:51.82 Intake.open @13:56:52.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:56:55.20 Vacuum tare is 56% of full scale @13:56:55.45 Pressure.in :default @13:56:55.56 Pressure.hold @13:56:55.85 SamplePump.in :prime @13:56:55.97 SamplePump.seek 50ml,2:53 @13:57:57.03 Pressure.stop @13:57:57.13 SamplePump.setPosition! 0ml @13:57:57.21 Exhaust.open @13:57:58.70 Bypass.close @13:58:00.32 Pressure.in :default @13:58:00.43 Pressure.hold @13:58:00.79 SamplePump.in :prime @13:58:00.90 SamplePump.seek 150ml,7:39 @14:01:03.35 Pressure.stop BACsampler @14:01:15.10 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @14:01:15.89 -> # [56,54] => { Type: :archiveHiBiomass_bac, @14:01:16.04 -> # State: :processed, @14:01:16.15 -> # filtered: 1000}, @14:01:16.29 -> # 55 => { Type: :microSPR_phyto, @14:01:16.40 -> # State: :lysing, @14:01:16.54 -> # filtered: 1000, @14:01:16.67 -> # spr: "spr23aug0707h.spr.bz2"}, @14:01:16.80 -> # 57 => { Type: :microSPR_phyto, @14:01:16.91 -> # State: :processed, @14:01:17.02 -> # filtered: 50, @14:01:17.12 -> # lysed: 825, @14:01:17.24 -> # spr: "spr23aug0704h.spr.bz2"}, @14:01:17.39 -> # 58 => { Type: :archiveHiBiomass_bac, @14:01:17.54 -> # State: :processed, @14:01:17.65 -> # filtered: 1000, @14:01:17.77 -> # was: :dry}, @14:01:17.90 -> # 59 => { Type: :microSPR_phyto, @14:01:18.04 -> # State: :processed, @14:01:18.14 -> # filtered: 50, @14:01:18.24 -> # lysed: 825, @14:01:18.38 -> # spr: "spr23aug0700h.spr.bz2", @14:01:18.48 -> # was: :dry}, @14:01:18.62 -> # 60 => { Type: :microSPR_phyto, @14:01:18.74 -> # State: :processed, @14:01:18.85 -> # filtered: 50, @14:01:18.96 -> # lysed: 825, @14:01:19.11 -> # spr: "spr23aug0623h.spr.bz2", @14:01:19.22 -> # was: :dry}} @14:01:19.34 -> #@13:38:38.38 -> show log @14:01:19.67 -> #@13:38:38.42 NoMethodError in errera -- undefined method `show' for CmdServer:: Client:Class @14:01:19.80 -> #@13:38:55.66 -> showlog @14:01:19.93 -> @13:39:39.32 -> Can @14:01:19.96 SyntaxError in errera -- compile error errera:36: `@1' is not allowed as an instance variable name @14:01:43.35 -> # [56,54] => { Type: :archiveHiBiomass_bac, @14:01:43.51 -> # State: :processed, @14:01:43.61 -> # filtered: 1000}, @14:01:43.76 -> # 55 => { Type: :microSPR_phyto, @14:01:43.89 -> # State: :lysing, @14:01:43.99 -> # filtered: 1000, @14:01:44.14 -> # spr: "spr23aug0707h.spr.bz2"}, @14:01:44.26 -> # 57 => { Type: :microSPR_phyto, @14:01:44.38 -> # State: :processed, @14:01:44.49 -> # filtered: 50, @14:01:44.59 -> # lysed: 825, @14:01:44.72 -> # spr: "spr23aug0704h.spr.bz2"}, @14:01:44.87 -> # 58 => { Type: :archiveHiBiomass_bac, @14:01:44.98 -> # State: :processed, @14:01:45.08 -> # filtered: 1000, @14:01:45.21 -> # was: :dry}, @14:01:45.34 -> # 59 => { Type: :microSPR_phyto, @14:01:45.50 -> # State: :processed, @14:01:45.61 -> # filtered: 50, @14:01:45.72 -> # lysed: 825, @14:01:45.83 -> # spr: "spr23aug0700h.spr.bz2", @14:01:45.93 -> # was: :dry}, @14:01:46.07 -> # 60 => { Type: :microSPR_phyto, @14:01:46.19 -> # State: :processed, @14:01:46.30 -> # filtered: 50, @14:01:46.40 -> # lysed: 825, @14:01:46.55 -> # spr: "spr23aug0623h.spr.bz2", @14:01:46.66 -> # was: :dry}} @14:01:46.78 -> #@13:38:38.38 -> show log @14:01:47.06 -> #@13:38:38.42 NoMethodError in errera -- undefined method `show' for CmdServer:: Client:Class @14:01:47.16 -> #@13:38:55.66 -> showlog @14:01:47.30 -> @13:39:39.32 -> Can @14:01:47.33 SyntaxError in errera -- compile error errera:68: `@1' is not allowed as an instance variable name @14:02:11.58 -> # [56,54] => { Type: :archiveHiBiomass_bac, @14:02:11.76 -> # State: :processed, @14:02:11.87 -> # filtered: 1000}, @14:02:12.02 -> # 55 => { Type: :microSPR_phyto, @14:02:12.31 -> # State: :lysing, @14:02:12.43 -> # filtered: 1000, @14:02:12.55 -> # spr: "spr23aug0707h.spr.bz2"}, @14:02:12.67 -> # 57 => { Type: :microSPR_phyto, @14:02:12.79 -> # State: :processed, @14:02:12.90 -> # filtered: 50, @14:02:13.02 -> # lysed: 825, @14:02:13.15 -> # spr: "spr23aug0704h.spr.bz2"}, @14:02:13.29 -> # 58 => { Type: :archiveHiBiomass_bac, @14:02:13.46 -> # State: :processed, @14:02:13.58 -> # filtered: 1000, @14:02:13.69 -> # was: :dry}, @14:02:14.00 -> # 59 => { Type: :microSPR_phyto, @14:02:14.12 -> # State: :processed, @14:02:14.24 -> # filtered: 50, @14:02:14.35 -> # lysed: 825, @14:02:14.50 -> # spr: "spr23aug0700h.spr.bz2", @14:02:14.61 -> # was: :dry}, @14:02:14.75 -> # 60 => { Type: :microSPR_phyto, @14:02:14.88 -> # State: :processed, @14:02:14.98 -> # filtered: 50, @14:02:15.09 -> # lysed: 825, @14:02:15.24 -> # spr: "spr23aug0623h.spr.bz2", @14:02:15.35 -> # was: :dry}} @14:02:15.51 -> #@13:38:38.38 -> show log @14:02:15.79 -> #@13:38:38.42 NoMethodError in errera -- undefined method `show' for CmdServer:: Client:Class @14:02:15.91 -> #@13:38:55.66 -> showlog @14:02:16.04 -> @13:39:39.32 -> Can @14:02:16.08 SyntaxError in errera -- compile error errera:100: `@1' is not allowed as an instance variable name @14:03:46.51 -> exit @14:03:46.55 Session Ended @14:04:47.47 Session Begun @14:05:19.79 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @14:05:59.57 -> Can Can@14:04:34, 25.0C, 36% humidity, 14.3psia, 14.859V, 0.420A, 0.275Ah, 6.24W @14:08:34.00 Session Begun @14:09:30.43 -> fire :archiveHiBiomass_bac, :microSPR_phyto @14:09:31.36 Toroid.seek :clear @14:09:31.54 Carousel.in :default @14:09:31.74 Carousel.home.seek 55 @14:09:32.61 Selecting Cartridge 49 @14:09:33.00 Carousel.seek 49 @14:09:34.63 Lysis1.in :fast @14:09:34.76 Lysis1.seek :locked @14:09:37.48 Plunge.seek :locked @14:09:37.73 Air.in :fast @14:09:37.84 Air.seek :locked @14:09:39.47 Carousel.reconfigure :locked @14:09:39.70 Powering cartridge bus @14:09:42.37 Gate.setIR 1750,5000,0,6 @14:09:42.50 Gate.setIR 0,5000,0,6 @14:09:42.62 Gate.power :cartridge,:OFF @14:09:43.22 Mounted archive Cartridge @14:09:43.68 Preparing to filter cartridge 49 @14:09:44.00 Bypass.open @14:09:44.78 Intake.close @14:09:45.63 Toroid.in :hold @14:09:45.76 Toroid.seek :crack @14:09:47.35 Toroid.seek :cartridge @14:09:48.54 Toroid.in :default @14:09:48.66 Toroid.seek :clear @14:09:50.74 Pressurizing cartridge to 26psi above 13.8psia @14:09:50.76 SamplePump.setPosition! 0ml @14:09:50.89 Intake.open @14:09:51.58 Bypass.close @14:09:52.35 Exhaust.close @14:09:53.25 Pressure.in :default @14:09:53.36 Pressure.hold @14:09:53.70 SamplePump.in :charge @14:09:53.83 SamplePump.seek 2.3ml,42 seconds @14:09:59.67 Pressure.stop @14:09:59.80 Intake.close @14:10:00.60 Pressurized cartridge with 0.149ml to 26.1psi @14:10:00.61 Isolated @34.5psia (20.7psi) @14:10:00.67 Verifying cartridge holds at least 0.65..0.85 of 34.5psia @14:10:00.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:10:07.90 Cartridge pressure fell 9% to 31.2psia @14:10:08.07 Sampling 1000.0ml @14:10:08.10 SamplePump.setPosition! 0ml @14:10:08.24 Exhaust.open @14:10:09.00 Intake.open @14:10:09.88 Pressure.in :default @14:10:10.00 Pressure.hold @14:10:10.19 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:10:10.45 SamplePump.in :sample @14:10:10.57 SamplePump.seek 1000ml,1:23:51 @14:10:13.58 Cartridge 49 filtering 1/1000ml at 0.60ml/s with 31.3psi vacuum @14:11:13.58 Cartridge 49 filtering 35/1000ml at 0.53ml/s with 28.9psi vacuum @14:11:14.65 Session Ended @14:12:13.58 Cartridge 49 filtering 66/1000ml at 0.51ml/s with 28.3psi vacuum @14:13:13.58 Cartridge 49 filtering 95/1000ml at 0.43ml/s with 26.9psi vacuum @14:13:45.08 Session Begun @14:13:59.36 -> Power.sampler true @14:14:13.58 Cartridge 49 filtering 122/1000ml at 0.46ml/s with 13.6psi vacuum 2.5m deep (18.3psia) @14:15:13.59 Cartridge 49 filtering 148/1000ml at 0.46ml/s with 27.0psi vacuum @14:16:13.58 Cartridge 49 filtering 173/1000ml at 0.42ml/s with 30.2psi vacuum @14:17:13.58 Cartridge 49 filtering 197/1000ml at 0.40ml/s with 30.8psi vacuum @14:17:16.14 Session Ended @14:18:13.59 Cartridge 49 filtering 220/1000ml at 0.38ml/s with 20.8psi vacuum @14:19:13.58 Cartridge 49 filtering 242/1000ml at 0.32ml/s with 24.7psi vacuum @14:20:13.59 Cartridge 49 filtering 262/1000ml at 0.30ml/s with 27.3psi vacuum @14:21:13.58 Cartridge 49 filtering 283/1000ml at 0.29ml/s with 21.4psi vacuum @14:21:28.37 -> showlog -100 @14:22:13.58 Cartridge 49 filtering 302/1000ml at 0.29ml/s with 18.6psi vacuum @14:23:13.58 Cartridge 49 filtering 320/1000ml at 0.34ml/s with 16.9psi vacuum @14:24:13.59 Cartridge 49 filtering 337/1000ml at 0.25ml/s with 15.9psi vacuum @14:25:13.59 Cartridge 49 filtering 353/1000ml at 0.24ml/s with 13.5psi vacuum 2.4m deep (18.2psia) @14:26:13.58 Cartridge 49 filtering 368/1000ml at 0.21ml/s with 27.8psi vacuum @14:27:13.58 Cartridge 49 filtering 382/1000ml at 0.28ml/s with 16.6psi vacuum @14:28:13.58 Cartridge 49 filtering 396/1000ml at 0.24ml/s with 26.8psi vacuum @14:29:13.58 Cartridge 49 filtering 409/1000ml at 0.20ml/s with 25.1psi vacuum @14:29:46.01 Flow averaged 0.35ml/s over 19:36 @14:29:46.10 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @14:29:47.27 Relieved excess vacuum in 1.2 seconds @14:29:47.34 SamplePump.seek 1000ml,49:08 @14:30:13.58 Cartridge 49 filtering 422/1000ml at 0.20ml/s with 29.3psi vacuum @14:30:40.34 Flow averaged 0.22ml/s over 54 seconds @14:30:40.42 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @14:30:41.05 Relieved excess vacuum in .7 seconds @14:30:41.11 SamplePump.seek 1000ml,48:10 @14:31:03.93 Flow averaged 0.22ml/s over 23 seconds @14:31:04.01 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @14:31:04.64 Relieved excess vacuum in .7 seconds @14:31:04.72 SamplePump.seek 1000ml,47:45 @14:31:13.57 Cartridge 49 filtering 435/1000ml at 0.20ml/s with 27.8psi vacuum @14:31:23.59 Flow averaged 0.21ml/s over 19 seconds @14:31:23.68 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @14:31:24.29 Relieved excess vacuum in .7 seconds @14:31:24.37 SamplePump.seek 1000ml,47:25 @14:31:35.96 Flow averaged 0.21ml/s over 12 seconds @14:31:36.06 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @14:31:36.66 Relieved excess vacuum in .7 seconds @14:31:36.74 SamplePump.seek 1000ml,47:13 @14:31:49.94 Flow averaged 0.21ml/s over 14 seconds @14:31:50.03 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @14:31:50.65 Relieved excess vacuum in .7 seconds @14:31:50.71 SamplePump.seek 1000ml,46:59 @14:32:00.96 Flow averaged 0.21ml/s over 11 seconds @14:32:01.06 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @14:32:01.67 Relieved excess vacuum in .7 seconds @14:32:01.74 SamplePump.seek 1000ml,46:48 @14:32:09.20 Flow estimate reduced to 0.17ml/s @14:32:09.23 Intake.close @14:32:09.33 Exhaust.close @14:32:09.73 Toroid.in :hold @14:32:09.86 Toroid.seek :bypass @14:32:11.55 Toroid.in :default @14:32:11.69 Toroid.seek :clear @14:32:13.34 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:32:13.37 Pressure.stop @14:32:13.73 Sampled 446.1ml @14:32:14.50 Plunge.seek :reagent @14:32:15.35 Sample loop closed. Ready to Deliver Reagents @14:32:15.37 Archive.seek :sealed @14:32:16.93 Gate.power :sampler,:OFF @14:32:19.44 Archive.seek :evac @14:32:33.55 delay 1:00 @14:32:53.12 Session Begun @14:33:33.58 Archive.seek :clear @14:33:51.13 RNAlater soak for 10:00 @14:43:51.17 Archive.seek :tVent @14:43:53.69 delay 1 second @14:43:54.71 Archive.seek :evac @14:44:10.28 delay 10 seconds @14:44:20.32 Archive.seek :tVent @14:44:35.90 delay 1 second @14:44:36.92 Archive.seek :evac @14:44:52.50 delay 10 seconds @14:45:02.53 Sample preserved and evacuated @14:45:02.78 Selecting Cartridge 48 @14:45:03.10 Carousel.in :default @14:45:03.30 Carousel.home.seek 49 @14:45:03.73 Archive.in :unlocking @14:45:03.97 Archive.seek :clear @14:45:04.08 Air.in :unlocking @14:45:04.34 Air.seek :clear @14:45:08.66 Lysis1.in :unlocking @14:45:08.88 Lysis1.seek :clear @14:45:09.01 Plunge.in :unlocking @14:45:09.18 Plunge.seek :clear @14:45:10.65 Carousel.seek 48 @14:45:11.25 Lysis1.in :fast @14:45:11.39 Lysis1.seek :locked @14:45:14.12 Plunge.in :default @14:45:14.29 Plunge.seek :locked @14:45:14.47 Air.in :fast @14:45:14.58 Air.seek :locked @14:45:16.20 Carousel.reconfigure :locked @14:45:16.45 Powering cartridge bus @14:45:19.13 Gate.setIR 1750,5000,0,6 @14:45:19.26 Gate.setIR 0,5000,0,6 @14:45:19.39 PuckHeater.configure :default @14:45:19.56 Gate.power :heat,:ON,limit to 5000mA @14:45:20.42 Mounted lysis Cartridge board#Q05044 @14:45:20.53 Using this cartridge's heat loss curve for HeatingAccelInCarousel @14:45:21.46 Preparing to filter cartridge 48 @14:45:21.77 Gate.power :sampler,:ON @14:45:21.93 Priming sample loop w/150ml, bypass w/50ml @14:45:21.96 SamplePump.setPosition! 0ml @14:45:22.09 Bypass.open @14:45:23.66 Intake.open @14:45:24.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:45:27.09 Vacuum tare is 56% of full scale @14:45:27.32 Pressure.in :default @14:45:27.49 Pressure.hold @14:45:27.77 Waiting for external sampler to finish priming... @14:45:27.80 delayUntil "14:48:51.80" @14:48:51.85 SamplePump.in :prime @14:48:51.98 SamplePump.seek 50ml,2:53 @14:49:53.03 Pressure.stop @14:49:53.15 SamplePump.setPosition! 0ml @14:49:53.25 Exhaust.open @14:49:54.74 Bypass.close @14:49:56.35 Pressure.in :default @14:49:56.47 Pressure.hold @14:49:56.85 SamplePump.in :prime @14:49:56.99 SamplePump.seek 150ml,7:39 @14:52:59.10 Pressure.stop @14:52:59.24 Bypass.open @14:52:59.99 Intake.close @14:53:00.86 Toroid.in :hold @14:53:00.99 Toroid.seek :crack @14:53:02.58 Toroid.seek :cartridge @14:53:03.75 Toroid.in :default @14:53:03.88 Toroid.seek :clear @14:53:05.95 Pressurizing cartridge to 26psi above 13.8psia @14:53:05.98 SamplePump.setPosition! 0ml @14:53:06.11 Intake.open @14:53:06.84 Bypass.close @14:53:07.59 Exhaust.close @14:53:08.51 Pressure.in :default @14:53:08.63 Pressure.hold @14:53:09.49 SamplePump.in :charge @14:53:09.62 SamplePump.seek 2.3ml,42 seconds @14:53:21.81 Pressure.stop @14:53:21.94 Intake.close @14:53:22.71 Pressurized cartridge with 0.667ml to 26.1psi @14:53:22.73 Isolated @33.8psia (20.0psi) @14:53:22.81 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @14:53:22.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:53:27.60 Sampling 50.0ml @14:53:27.63 SamplePump.setPosition! 0ml @14:53:27.78 Exhaust.open @14:53:28.56 Intake.open @14:53:29.49 Pressure.in :default @14:53:29.62 Pressure.hold @14:53:29.83 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:53:30.09 SamplePump.in :sample @14:53:30.22 SamplePump.seek 50ml,4:41 @14:53:33.23 Cartridge 48 filtering 1/50ml at 0.56ml/s with 16.0psi vacuum 1.8m deep (17.3psia) @14:54:33.22 Cartridge 48 filtering 31/50ml at 0.48ml/s with 24.1psi vacuum @14:55:15.04 Pressure.stop @14:55:15.30 Intake.close @14:55:15.44 Exhaust.close @14:55:15.79 Toroid.in :hold @14:55:15.93 Toroid.seek :bypass @14:55:17.62 Toroid.in :default @14:55:17.75 Toroid.seek :clear @14:55:19.44 Sampled 50.0ml @14:55:21.22 SPR.command "read_sequences" @14:55:21.63 SPR.command "stats_on" @14:55:21.84 SPR.command "start 0 1" @14:55:22.54 SPR.start /var/log/esp/spr23aug0714h.spr.bz2 @14:55:22.63 Gate.power :sampler,:OFF @14:55:24.48 Handoff.select :waste @14:55:31.26 Syringe tare is 18% of full scale @14:55:31.32 PinPort.in :locking @14:55:31.49 PinPort.seek :locked @14:55:34.10 Archive.in :fast @14:55:34.17 Lysis2.in :fast @14:55:34.31 Lysis1.seek 1350uL @14:55:34.57 Archive.seek :full @14:55:34.68 Lysis2.seek 950uL @14:55:39.49 Plunge.seek :reagent @14:55:40.47 Evacuating puck with filter bubble point of 50psi @14:55:40.54 PinPort.seek :locked @14:55:40.68 Handoff.select :waste @14:55:47.30 Air syringe pumping up to 1 strokes to reach 8psi @14:55:47.31 Air stroke #1 of 1 @14:55:47.54 Air.in :pressurize @14:55:47.70 Air.seek :pressurized @14:55:53.53 2.0psi after pressurize @14:55:53.60 Air.in :compress @14:55:53.72 Air.seek :evac @14:55:56.78 1.0psi after compress @14:56:07.38 0.8psi after compress (stable) @14:56:07.42 Air.in :default @14:56:07.65 Air.hold @14:56:07.97 at 0.3psi Opened to waste @14:56:07.99 Handoff.select :blocked @14:56:08.21 Archive syringe pumping up to 8 strokes to reach 50psi @14:56:08.23 Archive stroke #1 of 8 @14:56:08.44 Archive.in :pressurize @14:56:08.60 Archive.seek :pressurized @14:56:21.15 1.7psi after pressurize @14:56:26.75 1.7psi after pressurize (stable) @14:56:26.77 Archive.in :compress @14:56:26.95 Archive.seek :empty @14:56:41.68 14.2psi after compress @14:56:52.30 15.9psi after compress (stable) @14:56:52.32 Archive.in :gulp @14:56:52.49 Archive.seek :topVent @14:56:58.80 16.0psi after gulp @14:57:04.41 16.0psi after gulp (stable) @14:57:04.42 Archive stroke #2 of 8 @14:57:04.60 Archive.in :pressurize @14:57:04.72 Archive.seek :pressurized @14:57:17.28 15.3psi after pressurize @14:57:24.38 14.4psi after pressurize (stable) @14:57:24.41 Archive.in :compress @14:57:24.58 Archive.seek :empty @14:57:39.28 25.2psi after compress @14:58:31.43 15.7psi after compress (stable) @14:58:31.50 Archive.in :gulp @14:58:31.64 Archive.seek :topVent @14:58:38.01 15.6psi after gulp @14:58:43.67 15.5psi after gulp (stable) @14:58:43.69 Archive stroke #3 of 8 @14:58:43.89 Archive.in :pressurize @14:58:44.01 Archive.seek :pressurized @14:58:56.58 15.4psi after pressurize @14:59:02.18 15.5psi after pressurize (stable) @14:59:02.21 Archive.in :compress @14:59:02.37 Archive.seek :empty @14:59:17.04 27.7psi after compress @14:59:53.15 23.5psi after compress (stable) @14:59:53.18 Archive.in :gulp @14:59:53.35 Archive.seek :topVent @14:59:59.71 23.8psi after gulp @15:00:05.32 23.8psi after gulp (stable) @15:00:05.34 Archive stroke #4 of 8 @15:00:05.57 Archive.in :pressurize @15:00:05.69 Archive.seek :pressurized @15:00:18.29 23.8psi after pressurize @15:00:23.93 23.7psi after pressurize (stable) @15:00:23.97 Archive.in :compress @15:00:24.14 Archive.seek :empty @15:00:38.82 35.2psi after compress @15:00:49.94 37.1psi after compress (stable) @15:00:50.01 Archive.in :gulp @15:00:50.15 Archive.seek :topVent @15:00:56.45 37.4psi after gulp @15:01:02.07 37.4psi after gulp (stable) @15:01:02.08 Archive stroke #5 of 8 @15:01:02.27 Archive.in :pressurize @15:01:02.39 Archive.seek :pressurized @15:01:15.01 37.3psi after pressurize @15:01:20.67 37.3psi after pressurize (stable) @15:01:20.77 Archive.in :compress @15:01:20.98 Archive.seek :empty @15:01:35.72 48.0psi after compress @15:01:39.59 Reached 49.7 of 50.0psi after pumping 8650uL (5.00 strokes) @15:01:39.62 Archive.in :default @15:01:39.78 Archive.hold @15:01:39.91 Handoff.select :waste @15:01:51.18 delay 1:00 @15:02:51.20 Handoff.select :blocked @15:03:13.99 Handoff.select :waste @15:03:14.21 0.5psi at evacEnd @15:03:14.23 Handoff.select :blocked @15:03:14.46 Archive.in :fast @15:03:14.59 Archive.seek :topVent @15:03:28.36 Stable @0.6psi after Handoff blocked @15:03:28.40 Archive syringe pumping up to 8 strokes to reach 31psi @15:03:28.45 Archive stroke #1 of 8 @15:03:28.64 Archive.in :pressurize @15:03:28.77 Archive.seek :pressurized @15:03:41.32 0.7psi after pressurize @15:03:41.44 Archive.in :compress @15:03:41.60 Archive.seek :empty @15:03:56.29 12.0psi after compress @15:04:06.91 13.6psi after compress (stable) @15:04:06.98 Archive.in :gulp @15:04:07.17 Archive.seek :topVent @15:04:13.53 13.9psi after gulp @15:04:19.14 13.8psi after gulp (stable) @15:04:19.15 Archive stroke #2 of 8 @15:04:19.40 Archive.in :pressurize @15:04:19.58 Archive.seek :pressurized @15:04:32.15 13.8psi after pressurize @15:04:32.22 Archive.in :compress @15:04:32.35 Archive.seek :empty @15:04:47.03 24.9psi after compress @15:04:57.63 26.4psi after compress (stable) @15:04:57.72 Archive.in :gulp @15:04:57.83 Archive.seek :topVent @15:05:04.14 26.6psi after gulp @15:05:09.74 26.6psi after gulp (stable) @15:05:09.75 Archive stroke #3 of 8 @15:05:09.94 Archive.in :pressurize @15:05:10.07 Archive.seek :pressurized @15:05:22.62 26.5psi after pressurize @15:05:22.73 Archive.in :compress @15:05:22.86 Archive.seek :empty @15:05:32.73 Reached 30.8 of 30.6psi after pumping 5085uL (2.94 strokes) @15:05:32.76 Archive.in :default @15:05:32.97 Archive.hold @15:05:33.21 Handoff.select :waste @15:05:47.02 delay 1:00 @15:06:47.04 Handoff.select :blocked @15:07:09.83 Handoff.select :waste @15:07:10.01 0.7psi at evacEnd @15:07:10.08 Air.seek :fullVent @15:07:11.55 Archive.in :fast @15:07:11.68 Archive.seek :topVent @15:07:18.47 Seawater evacuated, Pressure above/below filter released. @15:07:18.64 Lysis1.in :slow @15:07:18.76 Lysis1.seek 1050uL @15:07:48.83 delay 5 seconds @15:07:53.86 Handoff.select :blocked @15:07:54.16 Lysis1.seek 450uL @15:08:54.16 delay 3 seconds @15:08:57.27 Air.in mix with 1.40048 parts Lysis1 @15:08:58.04 Air.seek :evac @15:08:58.11 Lysis1.seek 300uL @15:09:13.33 Lysis1.seek :empty @15:09:43.54 Lysis buffer delivered @9.6psi @15:09:43.56 Polling Can every 2:00 @15:09:44.18 PuckHeater.configure :default #ambient 25.7C @15:09:44.29 PuckHeater.seek 80.5C @15:10:34.44 PuckHeater.hold 2:00,80.5C @15:12:34.40 Polling Can every 2:00 @15:12:34.99 PuckHeater.configure :default #ambient 25.8C @15:12:35.14 PuckHeater.hold 30 seconds,79.4C @15:13:05.34 Polling Can every 2:00 @15:13:05.90 PuckHeater.configure :default #ambient 25.8C @15:13:06.02 PuckHeater.hold 30 seconds,78.9C @15:13:36.25 Polling Can every 2:00 @15:13:36.80 PuckHeater.configure :default #ambient 25.8C @15:13:36.92 PuckHeater.hold 10:00,78.4C @15:18:34.14 -> exit @15:18:34.17 Session Ended @15:19:20.56 Session Begun @15:23:36.48 Lysis complete. Syringe pressure @13.8psi @15:23:36.55 Archive syringe pumping up to 5 strokes to reach 40psi @15:23:36.58 Archive stroke #1 of 5 @15:23:36.76 Archive.in :pressurize @15:23:36.88 Archive.seek :pressurized @15:23:49.53 13.7psi after pressurize @15:23:55.14 13.6psi after pressurize (stable) @15:23:55.17 Archive.in :compress @15:23:55.33 Archive.seek :empty @15:24:09.54 Reached 40.5 of 40.0psi after pumping 1718uL (0.99 strokes) @15:24:09.56 Archive.in :default @15:24:09.73 Archive.hold @15:24:09.85 delay 2 seconds @15:24:11.89 Archive.in :fast @15:24:12.01 Archive.seek :full @15:24:19.25 Lysis1.seek 138uL @15:24:33.18 delay 1 second @15:24:34.21 Archive.seek :full @15:24:34.36 Lysis1.seek :empty @15:24:48.32 Handoff.select :blocked @15:24:48.50 Initial air pressure @42.8psi @15:24:48.63 Lysis1.in :extract @15:24:48.78 Lysis1.seek 600uL,nil @15:24:53.55 Archive syringe pumping up to 6 strokes to reach 25psi @15:24:53.56 Archive stroke #1 of 6 @15:24:53.80 Archive.in :pressurize @15:24:53.97 Archive.seek :pressurized @15:24:54.51 Archive.in :default @15:24:54.62 Archive.hold @15:24:55.02 Initial 600uL lysate @14.6psi @15:24:55.03 Handoff.select :blocked @15:24:55.24 Initial air pressure @15.2psi @15:24:55.34 Lysis1.seek 825uL,nil @15:24:55.72 Archive syringe pumping up to 6 strokes to reach 17psi @15:24:55.73 Archive stroke #1 of 6 @15:24:55.99 Archive.in :pressurize @15:24:56.15 Archive.seek :pressurized @15:25:08.17 14.6psi after pressurize @15:25:13.78 14.5psi after pressurize (stable) @15:25:13.85 Archive.in :compress @15:25:13.99 Archive.seek :empty @15:25:22.68 Archive.in :default @15:25:22.78 Archive.hold @15:25:23.21 Recovered 825uL lysate @16.7psi @15:25:23.24 Air.in :halfSpeed @15:25:23.46 Air.seek :fullVent @15:25:25.74 Puck Top Vented, bottom @6.8psi @15:25:25.75 Handoff.select :waste @15:25:26.01 delay 5 seconds @15:25:31.10 Handoff open to waste, bottom @0.6psi @15:25:31.13 Air.in :default @15:25:31.29 Air.seek :evac @15:25:32.75 Lysis2.in :DAmix @15:25:32.94 Lysis1.in :DAmix @15:25:33.65 Lysis2.seek 700uL @15:25:33.71 Lysis1.seek 575uL @15:26:08.90 delay 5 seconds @15:26:13.93 Handoff.select SPR @15:26:14.15 SPR.command "start 2 14" @15:26:15.47 SPR stabilized @15:26:15.70 delay 10 seconds @15:26:25.73 SPR.command "start 2 0" @15:26:28.85 Waiting up to 11:00 for SPR to enter state 1 @15:28:26.51 SPR.command "hold_on" @15:28:27.91 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:28:28.63 Lysis2.seek 400uL @15:28:28.69 Lysis1.seek 275uL @15:29:10.80 delay 15 seconds @15:29:25.82 SPR.command "hold_off" @15:29:29.08 Waiting up to 38:00 for SPR to enter state 7 @15:31:26.04 -> Can Can@15:30:18, 25.7C, 36% humidity, 14.3psia, 14.851V, 0.549A, 1.382Ah, 8.15W @15:33:17.72 -> exit @15:33:17.75 Session Ended @15:39:23.50 Session Ended @15:43:49.69 SPRsummary:62.4RIU,4e+09RIU,113RIU,114RIU,none,371ng/L,high after 3071s @15:43:49.72 Errno::EPIPE in ben#3's_reader -- Broken pipe @15:43:49.81 Session Ended @15:43:49.89 Thread::Aborted in PROCESSING -- "Aborted by ben#3" @15:43:49.93 Waiting to Resume from Checkpoint "Waiting up to 38:00 for SPR to enter state 7" @15:43:53.80 Thread::Aborted in SPRlogger -- "Aborted by ben#3" @15:43:53.83 Thread::ChildDied in PROCESSING -- :SPRlogger @15:43:53.84 Waiting to Resume from Checkpoint "Waiting up to 38:00 for SPR to enter state 7" @07:50:29.35PDT08-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @07:50:33.08 >BREAK< ESP3 Gateway boot v1.43 for core bus @07:50:33.11 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @07:50:36.74 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @07:50:37.37 Sea pressure (Abs) tare is 6% of full scale @07:50:46.35 Polling Can every 2:00 @07:50:46.42 Waiting for espclients... @07:51:56.53 Session Begun @07:52:32.80 -> ESP.ready! @07:52:32.86 Gate.power :sampler,:OFF,limit to 3500mA @07:52:32.88 Gate.power :main,:ON,limit to 5000mA @07:52:32.91 Gate.power :analytic1,:ON,limit to 5000mA @07:52:32.96 Gate.power :analytic2,:ON,limit to 5000mA @07:52:33.14 Powering core bus @07:52:35.38 SPR Module 1, FW Rev. B, FPGA Rev. C @07:52:35.93 Archive.configure :default @07:52:36.05 Air.configure :default @07:52:36.14 Lysis1.configure :default @07:52:36.26 Lysis2.configure :default @07:52:36.38 Plunge.configure :default @07:52:36.51 SamplePump.configure :default @07:52:36.62 Toroid.configure :default @07:52:36.75 Carousel.configure :default @07:52:36.86 Pressure.configure :default @07:52:36.98 ExtraPump.configure :default @07:52:37.07 PinPort.configure :default @07:52:37.16 All elf controllers running firmware version 4.84 @07:52:37.22 Gate.power :motor,:ON,limit to 5000mA @07:52:37.61 Archive.home.jog 0 @07:52:38.32 PinPort.home.jog 0 @07:52:38.38 Toroid.home.jog 0 @07:52:38.42 Plunge.home.jog 0 @07:52:38.46 Lysis1.home.jog 0 @07:52:38.51 Lysis2.home.jog 0 @07:52:38.55 Air.home.jog 0 @07:52:38.59 Carousel.home.jog 0 @07:52:39.88 Archive.in :homing @07:52:40.26 Toroid.in :homing @07:52:40.36 Lysis2.in :homing @07:52:40.42 Air.in :homing @07:52:40.47 Plunge.in :homing @07:52:40.53 Archive.jog 9117 @07:52:40.59 PinPort.in :homing @07:52:40.65 Lysis1.in :homing @07:52:40.99 Toroid.jog 614 @07:52:41.06 Air.jog 8995 @07:52:41.13 Lysis2.jog 7852 @07:52:41.17 Plunge.jog 2300 @07:52:41.21 PinPort.jog -2235 @07:52:41.26 Lysis1.jog 8427 @07:52:43.07 Plunge.setRawPosition! 50 @07:52:43.14 Plunge.seek :home @07:52:43.40 Toroid.setRawPosition! 0 @07:52:43.50 Plunge.in :default @07:52:43.53 Toroid.seek :home @07:52:43.74 Toroid.in :default @07:52:44.60 PinPort.setRawPosition! 0 @07:52:44.67 PinPort.seek :home @07:52:44.78 PinPort.in :default @07:52:45.62 Lysis2.setRawPosition! 50 @07:52:45.70 Lysis2.seek :home @07:52:45.81 Archive.setRawPosition! 50 @07:52:45.88 Archive.seek :home @07:52:46.00 Lysis1.setRawPosition! 50 @07:52:46.07 Lysis1.seek :home @07:52:46.21 Lysis2.in :default @07:52:46.29 Air.setRawPosition! 50 @07:52:46.43 Archive.in :default @07:52:46.47 Air.seek :home @07:52:46.67 Lysis1.in :default @07:52:46.83 Air.in :default @07:52:47.19 Toroid.seek :clear @07:52:48.83 Carousel.in :default @07:52:48.99 Carousel.home.seek 48 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 48 Pressure = Absolute Pressure (Leak) Sensor @14psia PinPort = Handoff Pin Port (PP) clear @07:53:09.83 -> Power.sampler :ON @07:53:09.86 Gate.power :sampler,:ON true @07:58:23.79 Session Begun @07:58:38.28 -> client benji @07:58:38.32 ArgumentError in ben -- wrong number of arguments (1 for 0) @07:58:50.79 -> exit @07:58:50.82 Session Ended @08:00:05.59 -> fire dry, dry @08:00:06.54 Toroid.seek :clear @08:00:06.66 Carousel.in :default @08:00:06.83 Carousel.home.seek 48 @08:00:07.14 Selecting Cartridge 47 @08:00:07.97 Carousel.seek 47 @08:00:08.56 Lysis1.in :fast @08:00:08.67 Lysis1.seek :locked @08:00:11.34 Plunge.seek :locked @08:00:11.54 Air.in :fast @08:00:11.63 Air.seek :locked @08:00:13.24 Carousel.reconfigure :locked @08:00:13.44 Powering cartridge bus @08:00:16.12 Gate.setIR 1750,5000,0,6 @08:00:16.27 Gate.setIR 0,5000,0,6 @08:00:16.38 Gate.power :cartridge,:OFF @08:00:16.96 Mounted archive Cartridge @08:00:17.34 Preparing to filter cartridge 47 @08:00:17.69 Priming sample loop w/150ml, bypass w/50ml @08:00:17.71 SamplePump.setPosition! 0ml @08:00:17.80 Bypass.open @08:00:19.34 Intake.open @08:00:20.18 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:00:22.77 Vacuum tare is 56% of full scale @08:00:22.97 Pressure.in :default @08:00:23.05 Pressure.hold @08:00:23.33 SamplePump.in :prime @08:00:23.43 SamplePump.seek 50ml,2:53 @08:01:24.44 Pressure.stop @08:01:24.52 SamplePump.setPosition! 0ml @08:01:24.60 Exhaust.open @08:01:26.11 Bypass.close @08:01:27.71 Pressure.in :default @08:01:27.80 Pressure.hold @08:01:28.14 SamplePump.in :prime @08:01:28.24 SamplePump.seek 150ml,7:39 @08:03:06.41 Session Begun @08:03:11.13 -> Can Can@08:02:44, 23.1C, 40% humidity, 14.4psia, 14.818V, 0.915A, 0.108Ah, 13.56W @08:04:30.62 Pressure.stop @08:04:30.79 Bypass.open @08:04:31.54 Intake.close @08:04:32.88 Toroid.in :hold @08:04:32.99 Toroid.seek :crack @08:04:34.58 Toroid.seek :cartridge @08:04:35.73 Toroid.in :default @08:04:35.84 Toroid.seek :clear @08:04:37.90 Pressurizing cartridge to 26psi above 13.7psia @08:04:37.92 SamplePump.setPosition! 0ml @08:04:38.04 Intake.open @08:04:38.71 Bypass.close @08:04:39.45 Exhaust.close @08:04:40.37 Pressure.in :default @08:04:40.47 Pressure.hold @08:04:40.81 SamplePump.in :charge @08:04:40.94 SamplePump.seek 2.3ml,42 seconds @08:04:48.01 Pressure.stop @08:04:48.12 Intake.close @08:04:48.87 Pressurized cartridge with 0.221ml to 26.3psi @08:04:48.89 Isolated @34.1psia (20.4psi) @08:04:48.91 Verifying cartridge holds at least 0.65..0.85 of 34.1psia @08:04:49.02 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:04:53.62 Cartridge pressure fell 1% to 33.7psia @08:04:53.76 Sampling 1000.0ml @08:04:53.78 SamplePump.setPosition! 0ml @08:04:53.90 Exhaust.open @08:04:54.68 Intake.open @08:04:55.53 Pressure.in :default @08:04:55.63 Pressure.hold @08:04:55.82 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:04:56.06 SamplePump.in :sample @08:04:56.19 SamplePump.seek 1000ml,1:23:51 @08:04:59.20 Cartridge 47 filtering 1/1000ml at 0.59ml/s with 28.9psi vacuum @08:05:59.20 Cartridge 47 filtering 34/1000ml at 0.54ml/s with 17.6psi vacuum @08:06:59.20 Cartridge 47 filtering 65/1000ml at 0.51ml/s with 23.7psi vacuum @08:07:59.20 Cartridge 47 filtering 94/1000ml at 0.50ml/s with 30.7psi vacuum @08:08:59.20 Cartridge 47 filtering 123/1000ml at 0.45ml/s with 27.2psi vacuum @08:09:57.01 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 47 => { Type: :archiveHiBiomass_bac, State: :filtering, TV: :open}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @08:09:59.19 Cartridge 47 filtering 150/1000ml at 0.47ml/s with 18.3psi vacuum 2.1m deep (17.8psia) @08:10:59.20 Cartridge 47 filtering 176/1000ml at 0.43ml/s with 27.2psi vacuum @08:11:59.72 Cartridge 47 filtering 202/1000ml at 0.40ml/s with 29.6psi vacuum @08:12:59.21 Cartridge 47 filtering 226/1000ml at 0.42ml/s with 28.8psi vacuum @08:13:59.20 Cartridge 47 filtering 249/1000ml at 0.41ml/s with 23.3psi vacuum @08:14:59.19 Cartridge 47 filtering 272/1000ml at 0.38ml/s with 31.2psi vacuum @08:15:59.20 Cartridge 47 filtering 293/1000ml at 0.37ml/s with 30.1psi vacuum @08:16:59.20 Cartridge 47 filtering 314/1000ml at 0.35ml/s with 29.7psi vacuum @08:17:59.20 Cartridge 47 filtering 334/1000ml at 0.33ml/s with 29.4psi vacuum @08:18:59.21 Cartridge 47 filtering 353/1000ml at 0.31ml/s with 30.4psi vacuum @08:19:56.78 Session Ended @08:19:59.20 Cartridge 47 filtering 371/1000ml at 0.29ml/s with 12.3psi vacuum 4.7m deep (21.7psia) @08:20:59.20 Cartridge 47 filtering 387/1000ml at 0.28ml/s with 29.4psi vacuum @08:21:59.20 Cartridge 47 filtering 403/1000ml at 0.22ml/s with 22.5psi vacuum @08:22:59.20 Cartridge 47 filtering 418/1000ml at 0.32ml/s with 25.7psi vacuum @08:23:59.19 Cartridge 47 filtering 433/1000ml at 0.20ml/s with 32.5psi vacuum @08:24:06.61 Session Begun @08:24:11.23 -> Can Can@08:22:41, 23.8C, 40% humidity, 14.4psia, 14.826V, 0.812A, 0.384Ah, 12.04W @08:24:18.10 -> exit @08:24:18.13 Session Ended @08:24:59.19 Cartridge 47 filtering 446/1000ml at 0.20ml/s with 27.6psi vacuum @08:25:59.20 Cartridge 47 filtering 460/1000ml at 0.23ml/s with 18.8psi vacuum 3.1m deep (19.2psia) @08:26:20.77 Flow averaged 0.36ml/s over 21:25 @08:26:20.83 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @08:26:21.44 Relieved excess vacuum in .7 seconds @08:26:21.48 SamplePump.seek 1000ml,45:09 @08:26:55.86 Flow averaged 0.22ml/s over 35 seconds @08:26:55.93 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @08:26:56.53 Relieved excess vacuum in .7 seconds @08:26:56.57 SamplePump.seek 1000ml,44:32 @08:26:59.20 Cartridge 47 filtering 472/1000ml at 0.20ml/s with 28.4psi vacuum @08:27:15.27 Flow averaged 0.21ml/s over 19 seconds @08:27:15.33 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @08:27:15.96 Relieved excess vacuum in .7 seconds @08:27:16.02 SamplePump.seek 1000ml,44:12 @08:27:32.03 Flow averaged 0.21ml/s over 17 seconds @08:27:32.10 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @08:27:32.72 Relieved excess vacuum in .7 seconds @08:27:32.77 SamplePump.seek 1000ml,43:56 @08:27:40.35 Flow estimate reduced to 0.17ml/s @08:27:40.38 Intake.close @08:27:40.47 Exhaust.close @08:27:40.78 Toroid.in :hold @08:27:40.88 Toroid.seek :bypass @08:27:42.55 Toroid.in :default @08:27:42.65 Toroid.seek :clear @08:27:44.30 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:27:44.33 Pressure.stop @08:27:45.20 Sampled 480.6ml @08:27:45.93 Plunge.seek :reagent @08:27:46.77 Sample loop closed. Ready to Deliver Reagents @08:27:46.80 Archive.seek :sealed @08:27:48.37 Gate.power :sampler,:OFF @08:27:50.78 Archive.seek :evac @08:28:04.85 delay 1:00 @08:29:04.88 Archive.seek :clear @08:29:22.40 RNAlater soak for 10:00 @08:32:16.94 Session Begun @08:39:22.44 Archive.seek :tVent @08:39:24.94 delay 1 second @08:39:25.97 Archive.seek :evac @08:39:41.49 delay 10 seconds @08:39:51.53 Archive.seek :tVent @08:40:07.08 delay 1 second @08:40:08.11 Archive.seek :evac @08:40:23.66 delay 10 seconds @08:40:33.70 Sample preserved and evacuated @08:40:33.87 Selecting Cartridge 46 @08:40:34.19 Carousel.in :default @08:40:34.40 Carousel.home.seek 47 @08:40:34.81 Archive.in :unlocking @08:40:35.10 Archive.seek :clear @08:40:35.16 Air.in :unlocking @08:40:35.44 Air.seek :clear @08:40:39.76 Lysis1.in :unlocking @08:40:39.98 Lysis1.seek :clear @08:40:40.08 Plunge.in :unlocking @08:40:40.26 Plunge.seek :clear @08:40:41.73 Carousel.seek 46 @08:40:42.36 Lysis1.in :fast @08:40:42.47 Lysis1.seek :locked @08:40:45.16 Plunge.in :default @08:40:45.27 Plunge.seek :locked @08:40:45.47 Air.in :fast @08:40:45.57 Air.seek :locked @08:40:47.18 Carousel.reconfigure :locked @08:40:47.40 Powering cartridge bus @08:40:50.10 Gate.setIR 1750,5000,0,6 @08:40:50.24 Gate.setIR 0,5000,0,6 @08:40:50.38 PuckHeater.configure :default @08:40:50.49 Gate.power :heat,:ON,limit to 5000mA @08:40:51.75 Mounted lysis Cartridge board#Q05076 @08:40:51.83 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:40:52.26 Preparing to filter cartridge 46 @08:40:52.58 Gate.power :sampler,:ON @08:40:52.72 Priming sample loop w/150ml, bypass w/50ml @08:40:52.75 SamplePump.setPosition! 0ml @08:40:52.85 Bypass.open @08:40:54.42 Intake.open @08:40:55.24 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:40:57.83 Vacuum tare is 56% of full scale @08:40:58.15 Pressure.in :default @08:40:58.29 Pressure.hold @08:40:58.57 Waiting for external sampler to finish priming... @08:40:58.59 delayUntil "08:44:22.60" @08:44:22.65 SamplePump.in :prime @08:44:22.77 SamplePump.seek 50ml,2:53 @08:45:23.79 Pressure.stop @08:45:23.94 SamplePump.setPosition! 0ml @08:45:24.03 Exhaust.open @08:45:25.57 Bypass.close @08:45:27.16 Pressure.in :default @08:45:27.27 Pressure.hold @08:45:27.63 SamplePump.in :prime @08:45:27.76 SamplePump.seek 150ml,7:39 @08:48:30.11 Pressure.stop @08:48:30.24 Bypass.open @08:48:31.04 Intake.close @08:48:31.91 Toroid.in :hold @08:48:32.02 Toroid.seek :crack @08:48:33.61 Toroid.seek :cartridge @08:48:34.77 Toroid.in :default @08:48:34.88 Toroid.seek :clear @08:48:36.94 Pressurizing cartridge to 26psi above 13.6psia @08:48:36.96 SamplePump.setPosition! 0ml @08:48:37.07 Intake.open @08:48:37.79 Bypass.close @08:48:38.53 Exhaust.close @08:48:39.41 Pressure.in :default @08:48:39.51 Pressure.hold @08:48:40.35 SamplePump.in :charge @08:48:40.46 SamplePump.seek 2.3ml,42 seconds @08:48:52.62 Pressure.stop @08:48:52.73 Intake.close @08:48:53.55 Pressurized cartridge with 0.663ml to 26.1psi @08:48:53.57 Isolated @33.8psia (20.2psi) @08:48:53.61 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @08:48:53.69 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:48:58.33 Cartridge pressure fell 1% to 33.5psia @08:48:58.47 Sampling 50.0ml @08:48:58.50 SamplePump.setPosition! 0ml @08:48:58.61 Exhaust.open @08:48:59.36 Intake.open @08:49:00.20 Pressure.in :default @08:49:00.34 Pressure.hold @08:49:00.52 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:49:00.76 SamplePump.in :sample @08:49:00.89 SamplePump.seek 50ml,4:41 @08:49:03.89 Cartridge 46 filtering 1/50ml at 0.59ml/s with 28.8psi vacuum @08:50:03.90 Cartridge 46 filtering 33/50ml at 0.53ml/s with 16.9psi vacuum 5.1m deep (22.2psia) @08:50:36.60 Pressure.stop @08:50:36.79 Intake.close @08:50:36.90 Exhaust.close @08:50:37.20 Toroid.in :hold @08:50:37.31 Toroid.seek :bypass @08:50:38.98 Toroid.in :default @08:50:39.10 Toroid.seek :clear @08:50:40.75 Sampled 50.0ml @08:50:42.51 SPR.command "read_sequences" @08:50:42.60 SPR.command "stats_on" @08:50:42.79 SPR.command "start 0 1" @08:50:43.49 SPR.start /var/log/esp/spr23aug0808h.spr.bz2 @08:50:43.92 Gate.power :sampler,:OFF @08:50:45.39 Handoff.select :waste @08:50:52.21 Syringe tare is 18% of full scale @08:50:52.31 PinPort.in :locking @08:50:52.42 PinPort.seek :locked @08:50:55.05 Archive.in :fast @08:50:55.14 Lysis2.in :fast @08:50:55.24 Lysis1.seek 1350uL @08:50:55.48 Archive.seek :full @08:50:55.57 Lysis2.seek 950uL @08:51:00.33 Plunge.seek :reagent @08:51:01.29 Evacuating puck with filter bubble point of 50psi @08:51:01.34 PinPort.seek :locked @08:51:01.48 Handoff.select :waste @08:51:08.10 Air syringe pumping up to 1 strokes to reach 8psi @08:51:08.12 Air stroke #1 of 1 @08:51:08.32 Air.in :pressurize @08:51:08.43 Air.seek :pressurized @08:51:14.30 1.3psi after pressurize @08:51:14.34 Air.in :compress @08:51:14.48 Air.seek :evac @08:51:17.54 1.3psi after compress @08:51:28.14 0.2psi after compress (stable) @08:51:28.16 Air.in :default @08:51:28.33 Air.hold @08:51:28.66 at 0.2psi Opened to waste @08:51:28.68 Handoff.select :blocked @08:51:28.88 Archive syringe pumping up to 8 strokes to reach 50psi @08:51:28.90 Archive stroke #1 of 8 @08:51:29.06 Archive.in :pressurize @08:51:29.18 Archive.seek :pressurized @08:51:41.79 0.9psi after pressurize @08:52:02.39 8.3psi after pressurize (stable) @08:52:02.44 Archive.in :compress @08:52:02.61 Archive.seek :empty @08:52:17.32 15.6psi after compress @08:52:27.93 16.4psi after compress (stable) @08:52:28.42 Archive.in :gulp @08:52:28.66 Archive.seek :topVent @08:52:34.96 16.5psi after gulp @08:52:40.56 16.4psi after gulp (stable) @08:52:40.58 Archive stroke #2 of 8 @08:52:40.74 Archive.in :pressurize @08:52:40.84 Archive.seek :pressurized @08:52:53.37 16.4psi after pressurize @08:53:09.48 22.0psi after pressurize (stable) @08:53:09.51 Archive.in :compress @08:53:09.69 Archive.seek :empty @08:53:24.36 30.2psi after compress @08:53:34.97 31.3psi after compress (stable) @08:53:35.00 Archive.in :gulp @08:53:35.18 Archive.seek :topVent @08:53:41.49 31.5psi after gulp @08:53:47.10 31.4psi after gulp (stable) @08:53:47.11 Archive stroke #3 of 8 @08:53:47.28 Archive.in :pressurize @08:53:47.38 Archive.seek :pressurized @08:53:59.98 31.2psi after pressurize @08:54:14.08 33.1psi after pressurize (stable) @08:54:14.14 Archive.in :compress @08:54:14.27 Archive.seek :empty @08:54:28.93 44.0psi after compress @08:54:39.56 45.1psi after compress (stable) @08:54:39.59 Archive.in :gulp @08:54:39.72 Archive.seek :topVent @08:54:46.02 45.2psi after gulp @08:54:51.62 45.2psi after gulp (stable) @08:54:51.64 Archive stroke #4 of 8 @08:54:51.84 Archive.in :pressurize @08:54:51.96 Archive.seek :pressurized @08:55:04.50 44.9psi after pressurize @08:55:10.11 44.9psi after pressurize (stable) @08:55:10.14 Archive.in :compress @08:55:10.32 Archive.seek :empty @08:55:19.31 Reached 50.2 of 50.0psi after pumping 6796uL (3.93 strokes) @08:55:19.33 Archive.in :default @08:55:19.39 Errno::EPIPE in ben's_reader -- Broken pipe @08:55:19.48 Session Ended @08:55:19.58 Thread::Aborted in PROCESSING -- Archive Syringe (ArS) "Aborted by ben" @08:55:19.63 Handoff.select :waste @08:55:21.10 Thread::Aborted in SPRlogger -- "Aborted by ben" @08:55:21.14 Handoff.select :blocked @08:55:21.32 Thread::ChildDied in PROCESSING -- :SPRlogger @08:55:21.34 Waiting to Resume from Checkpoint "Archive Syringe (ArS) "Aborted by ben"" @08:59:32.12 Session Begun @09:02:37.79 -> showlog -20 @09:04:44.14 -> showlog -100 @09:16:49.06 -> showlog -10 @09:17:27.79 -> exit @09:17:27.81 Session Ended @09:24:11.46 Session Begun @09:24:20.54 -> showlog-20 @09:24:20.61 NoMethodError in ben -- undefined method `-' for nil:NilClass @09:24:38.16 -> showlog -50 @09:25:04.80 -> Cmd.resume @09:25:04.84 Resuming Thread after Archive Syringe (ArS) "Aborted by ben" at 08:55:19 @09:25:04.90 Archive.in :default @09:25:05.02 Archive.hold @09:25:05.15 Handoff.select :waste @09:25:14.90 delay 1:00 @09:26:14.93 Handoff.select :blocked @09:26:37.70 Handoff.select :waste @09:26:37.92 0.5psi at evacEnd @09:26:37.94 Handoff.select :blocked @09:26:38.19 Archive.in :fast @09:26:38.34 Archive.seek :topVent @09:26:51.64 Stable @0.5psi after Handoff blocked @09:26:51.67 Archive syringe pumping up to 8 strokes to reach 31psi @09:26:51.70 Archive stroke #1 of 8 @09:26:52.34 Archive.in :pressurize @09:26:52.55 Archive.seek :pressurized @09:27:05.21 2.6psi after pressurize @09:27:05.27 Archive.in :compress @09:27:05.52 Archive.seek :empty @09:27:20.33 14.1psi after compress @09:27:30.94 14.6psi after compress (stable) @09:27:30.97 Archive.in :gulp @09:27:31.12 Archive.seek :topVent @09:27:37.42 14.6psi after gulp @09:27:43.02 14.6psi after gulp (stable) @09:27:43.03 Archive stroke #2 of 8 @09:27:43.21 Archive.in :pressurize @09:27:43.32 Archive.seek :pressurized @09:27:55.86 14.6psi after pressurize @09:27:55.89 Archive.in :compress @09:27:56.03 Archive.seek :empty @09:28:10.69 27.6psi after compress @09:28:21.29 28.1psi after compress (stable) @09:28:21.32 Archive.in :gulp @09:28:21.46 Archive.seek :topVent @09:28:27.76 28.2psi after gulp @09:28:33.38 28.1psi after gulp (stable) @09:28:33.40 Archive stroke #3 of 8 @09:28:33.57 Archive.in :pressurize @09:28:33.67 Archive.seek :pressurized @09:28:46.21 28.0psi after pressurize @09:28:46.29 Archive.in :compress @09:28:46.42 Archive.seek :empty @09:28:50.02 Reached 30.6 of 30.5psi after pumping 4951uL (2.86 strokes) @09:28:50.04 Archive.in :default @09:28:50.18 Archive.hold @09:28:50.34 Handoff.select :waste @09:29:05.11 delay 1:00 @09:30:05.14 Handoff.select :blocked @09:30:27.90 Handoff.select :waste @09:30:28.08 0.7psi at evacEnd @09:30:28.11 Air.seek :fullVent @09:30:29.61 Archive.in :fast @09:30:29.72 Archive.seek :topVent @09:30:36.62 Seawater evacuated, Pressure above/below filter released. @09:30:36.76 Lysis1.in :slow @09:30:36.87 Lysis1.seek 1050uL @09:31:06.91 delay 5 seconds @09:31:11.93 Handoff.select :blocked @09:31:12.19 Lysis1.seek 450uL @09:32:12.18 delay 3 seconds @09:32:15.30 Air.in mix with 1.40048 parts Lysis1 @09:32:16.02 Air.seek :evac @09:32:16.09 Lysis1.seek 300uL @09:32:31.31 Lysis1.seek :empty @09:33:01.45 Lysis buffer delivered @13.9psi @09:33:01.47 Polling Can every 2:00 @09:33:02.10 PuckHeater.configure :default #ambient 25.2C @09:33:02.20 PuckHeater.seek 79.6C @09:33:50.70 PuckHeater.hold 2:00,79.6C @09:35:50.58 Polling Can every 2:00 @09:35:51.13 PuckHeater.configure :default #ambient 25.3C @09:35:51.24 PuckHeater.hold 30 seconds,78.6C @09:36:21.41 Polling Can every 2:00 @09:36:21.97 PuckHeater.hold 30 seconds,78.1C @09:36:52.16 Polling Can every 2:00 @09:36:52.73 PuckHeater.hold 10:00,77.5C @09:44:52.92 Session Begun @09:44:57.20 -> Can Can@09:44:27, 25.2C, 38% humidity, 14.4psia, 14.833V, 0.724A, 1.271Ah, 10.74W @09:46:51.57 Lysis complete. Syringe pressure @14.8psi @09:46:51.61 Archive syringe pumping up to 5 strokes to reach 40psi @09:46:51.65 Archive stroke #1 of 5 @09:46:51.85 Archive.in :pressurize @09:46:51.98 Archive.seek :pressurized @09:47:04.53 14.7psi after pressurize @09:47:37.15 28.9psi after pressurize (stable) @09:47:37.18 Archive.in :compress @09:47:37.34 Archive.seek :empty @09:47:45.73 Reached 40.2 of 40.0psi after pumping 1594uL (0.92 strokes) @09:47:45.75 Archive.in :default @09:47:45.92 Archive.hold @09:47:46.06 delay 2 seconds @09:47:48.10 Archive.in :fast @09:47:48.22 Archive.seek :full @09:47:55.03 Lysis1.seek 138uL @09:48:08.96 delay 1 second @09:48:10.00 Archive.seek :full @09:48:10.12 Lysis1.seek :empty @09:48:24.18 Handoff.select :blocked @09:48:24.41 Initial air pressure @41.8psi @09:48:24.56 Lysis1.in :extract @09:48:24.74 Lysis1.seek 600uL,nil @09:48:29.06 Archive syringe pumping up to 6 strokes to reach 25psi @09:48:29.08 Archive stroke #1 of 6 @09:48:29.29 Archive.in :pressurize @09:48:29.44 Archive.seek :pressurized @09:48:30.49 Archive.in :default @09:48:30.65 Archive.hold @09:48:31.01 Initial 600uL lysate @12.9psi @09:48:31.02 Handoff.select :blocked @09:48:31.24 Initial air pressure @12.9psi @09:48:31.39 Lysis1.seek 825uL,nil @09:48:31.61 Archive syringe pumping up to 6 strokes to reach 17psi @09:48:31.63 Archive stroke #1 of 6 @09:48:32.61 Archive.in :pressurize @09:48:32.76 Archive.seek :pressurized @09:48:44.20 12.9psi after pressurize @09:48:49.06 Archive.in :default @09:48:49.14 Archive.hold @09:48:49.49 Recovered 825uL lysate @14.3psi @09:48:49.54 Air.in :halfSpeed @09:48:50.15 Air.seek :fullVent @09:48:52.57 Puck Top Vented, bottom @15.0psi @09:48:52.59 Handoff.select :waste @09:48:52.81 delay 5 seconds @09:48:57.91 Handoff open to waste, bottom @0.5psi @09:48:57.94 Air.in :default @09:48:58.10 Air.seek :evac @09:48:59.58 Lysis2.in :DAmix @09:48:59.78 Lysis1.in :DAmix @09:49:00.42 Lysis2.seek 700uL @09:49:00.48 Lysis1.seek 575uL @09:49:35.62 delay 5 seconds @09:49:40.65 Handoff.select SPR @09:49:40.84 SPR.command "start 2 14" @09:49:40.96 SPR stabilized @09:49:41.18 delay 10 seconds @09:49:51.23 AnalyticModule::SPR::Error in PROCESSING -- not started @09:49:51.26 Waiting to Resume from Checkpoint "Archive Syringe (ArS) pressureOutOfBounds at 136uL (sensed 148uL)" @09:55:30.49 -> showlog -5 @09:58:24.39 -> Can Can@09:56:25, 25.3C, 38% humidity, 14.4psia, 14.856V, 0.501A, 1.411Ah, 7.45W @10:01:30.71 Session Begun @10:01:38.16 -> showlog -50 @10:02:54.75 Session Begun @10:03:06.59 -> showlog 200 @10:03:57.40 -> Syringe.PSI 0.176312533742443 @10:04:35.17 -> progress >>> Thread[:MAIN] is sleeping <<< @07:50:46 Waiting for espclients... >>> Thread[:PROCESSING] is suspended <<< @08:51:01 Evacuating puck with filter bubble point of 50psi @08:51:08 Air syringe pumping up to 1 strokes to reach 8psi @08:51:28 Archive syringe pumping up to 8 strokes to reach 50psi @08:55:18 Archive Syringe (ArS) pressureOutOfBounds at 124uL (sensed 137uL) @08:55:19 Archive Syringe (ArS) "Aborted by ben" @09:26:51 Archive syringe pumping up to 8 strokes to reach 31psi @09:28:49 Archive Syringe (ArS) pressureOutOfBounds at 239uL (sensed 250uL) @09:46:51 Archive syringe pumping up to 5 strokes to reach 40psi @09:47:40 Archive Syringe (ArS) pressureOutOfBounds at 136uL (sensed 148uL) @10:05:20.19 -> SC Storage Carousel (SC) at 46 @10:07:17.46 -> #Ben, I'm looking into this. Standby. Looks like a cartridge problem... @10:07:42.22 -> #You can message me back by prefixing each line with a '#' sign. @10:08:33.44 -> Cmd.processing.exception.last.backtrace ["/home/esp/esp2/lib/analytic/uwspr.rb:350:in `chkStarted'", "/home/esp/esp2/lib/analytic/uwspr.rb:62:in `run'", "/home/esp/esp2/lib/analytic/uwspr.rb:69:in `runExperiment'", "/home/esp/esp2/protocol/3G/spr.rb:246:in `runSPR'", "/home/esp/esp2/lib/posix/serialport.rb:148:in `each_with_index'", "/home/esp/esp2/protocol/3G/spr.rb:235:in `each'", "/home/esp/esp2/protocol/3G/spr.rb:235:in `each_with_index'", "/home/esp/esp2/protocol/3G/spr.rb:235:in `runSPR'", "/home/esp/esp2/protocol/3G/spr.rb:201:in `doSPR'", "/home/esp/esp2/protocol/3G/spr.rb:218:in `runSPR'", "/home/esp/esp2/protocol/3G/spr.rb:37:in `microSPR'", "/home/esp/esp2/protocol/3G/common3Gcfg.rb:21:in `microSPR'", "/home/esp/esp2/utils/cartridge/common.rb:379:in `call'", "/home/esp/esp2/utils/cartridge/common.rb:379:in `process'", "/home/esp/esp2/utils/auv/cmd.rb:495:in `startProcessing!'", "/home/esp/esp2/lib/schedule.rb:497:in `initialize'", "/home/esp/esp2/lib/thread.rb:736:in `start'", "/home/esp/esp2/lib/schedule.rb:490:in `initialize'", "/home/esp/esp2/lib/schedule.rb:488"] @10:08:44.08 -> #Thanks! @10:09:03.73 Session Ended @10:09:20.97 -> # I will be on standby @10:12:59.47 -> SPR.status @10:12:59.56 NoMethodError in brent -- undefined method `status' for SPR:AnalyticModule::SPR @10:13:01.91 -> SPR # @10:13:09.79 -> SPR.stopped? true @10:14:38.39 -> showlog -200 @10:18:33.65 -> #Brent, are you currently on MainThread or are you logged in as a client? @10:18:52.22 -> #client @10:19:16.36 -> #I'm the client "brent" @10:19:46.14 -> progress >>> Thread[:MAIN] is sleeping <<< @07:50:46 Waiting for espclients... >>> Thread[:PROCESSING] is suspended <<< @08:51:01 Evacuating puck with filter bubble point of 50psi @08:51:08 Air syringe pumping up to 1 strokes to reach 8psi @08:51:28 Archive syringe pumping up to 8 strokes to reach 50psi @08:55:18 Archive Syringe (ArS) pressureOutOfBounds at 124uL (sensed 137uL) @08:55:19 Archive Syringe (ArS) "Aborted by ben" @09:26:51 Archive syringe pumping up to 8 strokes to reach 31psi @09:28:49 Archive Syringe (ArS) pressureOutOfBounds at 239uL (sensed 250uL) @09:46:51 Archive syringe pumping up to 5 strokes to reach 40psi @09:47:40 Archive Syringe (ArS) pressureOutOfBounds at 136uL (sensed 148uL) @10:20:33.80 -> SPR.command "start 2 14" @10:20:33.84 SPR.command "start 2 14" @10:20:43.84 -> Cmd.resume @10:20:44.00 Resuming Thread after Archive Syringe (ArS) pressureOutOfBounds at 136uL (sensed 148uL) at 09:47:40 @10:20:44.12 Archive.in :compress @10:20:44.29 Archive.seek :empty @10:20:59.02 1.2psi after compress @10:21:09.62 1.7psi after compress (stable) @10:21:09.76 Archive.in :gulp @10:21:09.97 Archive.seek :topVent @10:21:16.35 1.8psi after gulp @10:21:21.97 1.8psi after gulp (stable) @10:21:21.98 Archive stroke #2 of 5 @10:21:22.22 Archive.in :pressurize @10:21:22.41 Archive.seek :pressurized @10:21:35.09 3.8psi after pressurize @10:21:35.60 Session Begun @10:21:41.93 -> SPR.stopped? true @10:21:47.70 9.4psi after pressurize (stable) @10:21:47.81 Archive.in :compress @10:21:47.96 Archive.seek :empty @10:21:57.06 -> Handoff Handoff Valve::Manifold selects Analytical Module @10:22:02.69 14.1psi after compress @10:22:13.49 14.5psi after compress (stable) @10:22:13.59 Archive.in :gulp @10:22:13.76 Archive.seek :topVent @10:22:20.12 14.6psi after gulp @10:22:25.73 14.6psi after gulp (stable) @10:22:25.74 Archive stroke #3 of 5 @10:22:26.05 Archive.in :pressurize @10:22:26.22 Archive.seek :pressurized @10:22:31.84 -> hidelog Log display suppressed @10:22:38.86 14.9psi after pressurize @10:22:40.92 Reached 17.1 of 40.0psi after pumping 6471uL (3.74 strokes) @10:22:40.94 Archive.in :default @10:22:41.15 Archive.hold @10:22:41.33 delay 2 seconds @10:22:43.36 Archive.in :fast @10:22:43.50 Archive.seek :full @10:22:49.66 Lysis1.in :slow @10:22:49.80 Lysis1.seek 713uL @10:23:03.73 delay 1 second @10:23:04.76 Archive.seek :full @10:23:05.01 Lysis1.seek :empty @10:23:41.82 -> #Is there a way to see a list of active clients? the first two messages you sent didn't have the client name associated with it @10:24:16.51 Handoff.select :blocked @10:24:16.80 Initial air pressure @36.1psi @10:24:16.94 Lysis1.in :extract @10:24:17.17 Lysis1.seek 600uL,nil @10:24:22.87 Initial 600uL lysate @17.4psi @10:24:22.90 Handoff.select :blocked @10:24:23.13 Initial air pressure @17.7psi @10:24:23.28 Lysis1.seek 825uL,nil @10:24:24.78 Archive syringe pumping up to 6 strokes to reach 17psi @10:24:24.80 Archive stroke #1 of 6 @10:24:25.02 Archive.in :pressurize @10:24:25.21 Archive.seek :pressurized @10:24:26.03 Archive.in :default @10:24:26.12 Archive.hold @10:24:26.58 Recovered 825uL lysate @14.3psi @10:24:26.62 Air.in :halfSpeed @10:24:26.82 Air.seek :fullVent @10:24:29.12 Puck Top Vented, bottom @13.6psi @10:24:29.14 Handoff.select :waste @10:24:29.39 delay 5 seconds @10:24:34.48 Handoff open to waste, bottom @0.6psi @10:24:34.56 Air.in :default @10:24:34.70 Air.seek :evac @10:24:36.34 Lysis1.in :DAmix @10:24:37.05 Lysis2.seek 450uL @10:24:37.20 Lysis1.seek 575uL @10:25:07.49 Session Begun @10:25:12.41 delay 5 seconds @10:25:14.28 -> Can Can@10:24:21, 25.4C, 38% humidity, 14.4psia, 14.834V, 0.752A, 1.685Ah, 11.15W @10:25:17.44 Handoff.select SPR @10:25:17.66 SPR.command "start 2 14" @10:25:18.48 SPR stabilized @10:25:18.73 delay 10 seconds @10:25:28.78 AnalyticModule::SPR::Error in PROCESSING -- not started @10:25:28.80 Waiting to Resume from Checkpoint "Archive Syringe (ArS) pressureOutOfBounds at 136uL (sensed 148uL)" @10:26:01.80 Cmd::Error in brent -- Not Processing @10:26:01.93 UserAbort in brent -- User Abort!! @10:27:06.22 -> #I cannot easily recover from this. @10:27:34.99 -> #The initial break in WiFi that started this, caused the SPR to stop logging. @10:28:04.99 -> #That's what the system is complaining about when in the "not started" error above. @10:28:25.46 -> #I'm going to unlock this cartridge. @10:28:31.06 -> # Is there a way that we can get the process to keep working if connection is lost? That will likely happen more than once. @10:29:12.38 -> #You can. @10:29:38.75 -> #I sent email on about it a few days back. @10:30:19.91 -> #Short answer is: issue your long running commands as: @10:30:45.02 -> # start {fire :dry} @10:31:38.42 -> #I see now, does that need to be start {fire :dry, dry} for both catridges i.e archive and lysis? @10:31:40.95 -> #This runs the fire command in a separate thread @10:31:54.46 -> # understood thank you! @10:31:55.79 -> #yes. Exactly. @10:32:21.87 -> ExternalValves @10:32:21.97 NameError in brent -- uninitialized constant ExternalValves @10:32:25.68 -> ExternalValve Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed @10:32:33.09 -> Cart.unlock @10:32:33.23 Gate.power :heat,:OFF @10:32:33.25 Gate.power :cartridge,:OFF @10:32:33.51 PinPort.in :unlocking @10:32:33.70 PinPort.seek :clear @10:32:35.69 Carousel.in :default @10:32:35.92 Carousel.home.seek 46 @10:32:36.44 Archive.in :unlocking @10:32:36.71 Archive.seek :clear @10:32:36.79 Air.in :unlocking @10:32:37.21 Air.seek :clear @10:32:37.28 Lysis2.in :unlocking @10:32:37.69 Lysis2.seek :clear @10:32:41.70 Lysis1.in :unlocking @10:32:41.97 Lysis1.seek :clear @10:32:42.04 Plunge.in :unlocking @10:32:42.39 Plunge.seek :clear @10:32:47.92 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50.0, lysed: 825, spr: spr23aug0808h.spr.bz2}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @10:33:08.42 -> Cmd.stop @10:33:08.64 Handoff.select :close @10:33:08.98 Carousel.in :default @10:33:09.24 Carousel.home.seek 46 @10:33:09.71 Lysis1.in :fast @10:33:09.89 Lysis1.seek :locked @10:33:12.50 Plunge.in :default @10:33:12.66 Plunge.seek :locked @10:33:14.66 Lysis1.in :unlocking @10:33:15.05 Lysis1.seek :clear @10:33:16.66 Gate.power :motor,:OFF @10:33:16.70 Gate.power :core,:OFF @10:33:16.80 Gate.power :analytic1,:OFF @10:33:16.94 Gate.power :analytic2,:OFF @10:33:17.03 Gate.power :main,:OFF @10:33:17.10 Safely stopped and ready to power off :STOPPED @10:33:34.00 -> # so the lysis is no good, is there anything we need to do in person? We will be with the unit tomorrow. @10:33:35.40 -> #OK. You are ready to fire the next cartridges @10:34:05.97 -> #I just realized something. @10:34:32.15 -> #Are you doublets archive first then DA or DA then archival? @10:34:44.04 -> # archive first @10:35:05.53 -> # the archive sample was finished before the lost connection @10:35:12.34 -> #Good. Then the next dry cart should be an archive. @10:35:25.98 -> # ok great! @10:35:46.83 -> # while I have you is there a way to see the list of active clients? @10:35:53.09 -> clients [ESP["ben"],ESP["benji"],ESP["nadia"],ESP["brent"],ESP["brent2"],ESP["errera"]] @10:36:03.88 -> clients [ESP["ben"],ESP["benji"],ESP["nadia"],ESP["brent"],ESP["brent2"],ESP["errera"]] @10:36:11.50 -> # thanks! @10:36:40.91 -> # one last question @10:37:16.08 -> #There may be some usable lysate, but I pressurized it twice, so not much will be left. @10:37:23.05 -> #ask away @10:37:53.47 -> # if the cartridge is fired in a new thread is the operating client able to exit without disrupting the process? @10:38:26.46 -> #Yes. @10:38:33.00 -> e.g. using the start {fire} as opposed to fire @10:38:33.04 SyntaxError in benji -- compile error benji:20: syntax error, unexpected tIDENTIFIER, expecting $end e.g. using the start {fire} as opposed to fire ^ @10:38:44.17 -> # awesome that is excellent thanks! @10:39:51.68 -> exit @10:39:51.72 Session Ended @10:40:19.63 -> #I'll stay on-line for a bit. When will you fire the next doublet? @10:42:46.64 -> #not sure yet let me get back to you in just a minute @10:45:42.36 Session Ended @10:52:07.88 -> # we will fire the next doublet tomorrow morning. WE ware going to complete a transect starting around 8 to 9 am. @10:52:31.76 -> #OK, then I'll sign off for now. @10:52:37.55 -> # I really appreciate all of MBARI's help with this! @10:53:02.21 -> # wait one more question if you are still there @10:53:04.21 -> #If you message me an hour before your next doublet, I'll hop on to monitor. @10:53:10.96 -> #sure... @10:53:29.02 -> # ok I will; is there a way to export the log to a .txt? @10:53:42.93 -> SPR # @10:54:07.56 -> Power {analytic1: false, analytic2: false, cartridge: false, core: false, heat: false, main: false, motor: false, sampler: false} @10:55:20.28 -> #not sure what you mean. There's a .out file that's ASCII text of everything you see on your screen. @10:55:46.37 -> #and there's a real.log that's binary. @10:56:17.41 -> #There is a tool that converts the real.log to text called "dumplog" @10:56:50.37 -> upsync #<#::NoThread:0xb6ce0e00> @10:57:14.31 -> #the upsync command sends all the logs and data to the ESPshore server. @10:57:44.15 -> #The text log will always be accessible there at the link: @10:58:04.34 -> # http://ESPshore.mbari.org/honu/esp/real.out @11:02:29.09 -> # that is gret thanks! @11:02:29.19 Errno::EPIPE in ben's_reader -- Broken pipe @11:02:29.25 Session Ended @11:02:47.54 -> exit @11:02:47.57 Session Ended @11:04:21.57 Session Begun @11:04:32.21 -> showlog -20 @11:09:14.86 Session Ended @11:11:26.90 -> exit @11:11:26.92 Session Ended @11:24:39.78 -> Time.now 11:24:39.81PDT08-Aug-23 @11:42:18.57 -> exit @11:42:18.59 Session Ended @12:06:03.74 Session Begun @12:06:11.08 -> Can Can@12:06:04, 24.7C, 39% humidity, 14.4psia, 14.888V, 0.082A, 1.905Ah, 1.21W @12:06:49.15 -> showlog -200 @12:37:16.47 -> exit @12:37:16.49 Session Ended @19:22:31.43 Session Begun @22:22:45.09EDT08-Aug-23 -> showlog 200 @22:23:39.70 -> Time.now 22:23:39.72EDT08-Aug-23 @22:26:09.41 -> def startDoublet; start {fire :archiveHiBiomass_bac, :microSPR_phyto}; end :startDoublet @22:26:14.84 -> exit @22:26:14.88 Session Ended @22:26:17.16 Session Begun @22:26:26.00 -> method :startDoublet # @22:26:36.71 -> _.source brent:3 @22:28:31.47 -> exit @22:28:31.49 Session Ended @06:25:41.71EDT09-Aug-23 Session Begun @03:25:47.16PDT09-Aug-23 -> Can Can@03:25:10, 25.9C, 41% humidity, 14.4psia, 14.884V, 0.081A, 3.150Ah, 1.21W @03:39:07.81 Session Ended @05:26:53.60 Session Begun @05:27:08.52 -> Can Can@05:26:45, 25.9C, 41% humidity, 14.4psia, 14.884V, 0.082A, 3.315Ah, 1.22W @05:33:48.29 -> start {fire :dry, dry} # @05:33:48.55 Gate.power :sampler,:OFF,limit to 3500mA @05:33:48.58 Gate.power :main,:ON,limit to 5000mA @05:33:48.62 Gate.power :analytic1,:ON,limit to 5000mA @05:33:48.66 Gate.power :analytic2,:ON,limit to 5000mA @05:33:48.75 Powering core bus @05:33:51.07 SPR Module 1, FW Rev. B, FPGA Rev. C @05:33:51.53 Archive.configure :default @05:33:51.65 Air.configure :default @05:33:51.74 Lysis1.configure :default @05:33:51.83 Lysis2.configure :default @05:33:51.95 Plunge.configure :default @05:33:52.09 SamplePump.configure :default @05:33:52.26 Toroid.configure :default @05:33:52.41 Carousel.configure :default @05:33:52.54 Pressure.configure :default @05:33:53.22 ExtraPump.configure :default @05:33:53.32 PinPort.configure :default @05:33:53.42 All elf controllers running firmware version 4.84 @05:33:53.45 Gate.power :motor,:ON,limit to 5000mA @05:33:53.88 Archive.home.jog 0 @05:33:54.11 Air.home.jog 0 @05:33:54.76 Carousel.home.jog 0 @05:33:54.85 Toroid.home.jog 0 @05:33:54.92 Lysis2.home.jog 0 @05:33:54.98 Lysis1.home.jog 0 @05:33:55.02 Plunge.home.jog 0 @05:33:55.07 PinPort.home.jog 0 @05:33:55.90 Archive.in :homing @05:33:56.02 Air.in :homing @05:33:56.19 Archive.jog 1389 @05:33:56.23 Air.jog 1371 @05:33:56.60 PinPort.in :homing @05:33:56.64 Toroid.in :homing @05:33:56.70 Lysis2.in :homing @05:33:56.74 Lysis1.in :homing @05:33:56.83 Plunge.in :homing @05:33:57.06 PinPort.jog -107 @05:33:57.18 Toroid.jog 608 @05:33:57.21 Lysis2.jog 1393 @05:33:57.27 Lysis1.jog 1389 @05:33:57.32 Plunge.jog 1565 @05:33:57.85 Air.setRawPosition! 50 @05:33:57.88 Archive.setRawPosition! 50 @05:33:57.93 PinPort.setRawPosition! 0 @05:33:58.11 Air.seek :home @05:33:58.16 PinPort.seek :home @05:33:58.22 Archive.seek :home @05:33:58.58 PinPort.in :default @05:33:58.73 Air.in :default @05:33:58.85 Lysis2.setRawPosition! 50 @05:33:58.90 Archive.in :default @05:33:59.05 Lysis2.seek :home @05:33:59.19 Lysis1.setRawPosition! 50 @05:33:59.22 Plunge.setRawPosition! 50 @05:33:59.37 Lysis1.seek :home @05:33:59.40 Plunge.seek :home @05:33:59.64 Toroid.setRawPosition! 0 @05:33:59.66 Lysis2.in :default @05:33:59.81 Toroid.seek :home @05:33:59.99 Plunge.in :default @05:34:00.04 Lysis1.in :default @05:34:00.09 Toroid.in :default @05:34:00.58 Toroid.seek :clear @05:34:02.23 Carousel.in :default @05:34:02.44 Carousel.home.seek 46 @05:34:02.74 Selecting Cartridge 45 @05:34:03.64 Carousel.seek 45 @05:34:04.23 Lysis1.in :fast @05:34:04.37 Lysis1.seek :locked @05:34:07.03 Plunge.seek :locked @05:34:07.25 Air.in :fast @05:34:07.34 Air.seek :locked @05:34:08.96 Carousel.reconfigure :locked @05:34:09.16 Powering cartridge bus @05:34:11.85 Gate.setIR 1750,5000,0,6 @05:34:11.99 Gate.setIR 0,5000,0,6 @05:34:12.09 Gate.power :cartridge,:OFF @05:34:12.66 Mounted archive Cartridge @05:34:13.04 Preparing to filter cartridge 45 @05:34:13.32 Gate.power :sampler,:ON @05:34:13.45 Priming sample loop w/150ml, bypass w/50ml @05:34:13.47 SamplePump.setPosition! 0ml @05:34:13.55 Bypass.open @05:34:15.14 Intake.open @05:34:15.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:34:18.51 Vacuum tare is 56% of full scale @05:34:18.71 Pressure.in :default @05:34:18.81 Pressure.hold @05:34:19.07 Waiting for external sampler to finish priming... @05:34:19.10 delayUntil "05:37:43.34" @05:37:43.39 SamplePump.in :prime @05:37:43.50 SamplePump.seek 50ml,2:53 @05:38:44.50 Pressure.stop @05:38:44.59 SamplePump.setPosition! 0ml @05:38:44.66 Exhaust.open @05:38:46.14 Bypass.close @05:38:47.71 Pressure.in :default @05:38:47.80 Pressure.hold @05:38:48.14 SamplePump.in :prime @05:38:48.24 SamplePump.seek 150ml,7:39 @05:41:51.14 Pressure.stop @05:41:51.24 Bypass.open @05:41:51.98 Intake.close @05:41:52.80 Toroid.in :hold @05:41:52.90 Toroid.seek :crack @05:41:54.47 Toroid.seek :cartridge @05:41:55.60 Toroid.in :default @05:41:55.69 Toroid.seek :clear @05:41:57.76 Pressurizing cartridge to 26psi above 13.9psia @05:41:57.78 SamplePump.setPosition! 0ml @05:41:57.86 Intake.open @05:41:58.55 Bypass.close @05:41:59.28 Exhaust.close @05:42:00.17 Pressure.in :default @05:42:00.28 Pressure.hold @05:42:00.56 SamplePump.in :charge @05:42:00.67 SamplePump.seek 2.3ml,42 seconds @05:42:07.66 Pressure.stop @05:42:07.76 Intake.close @05:42:08.56 Pressurized cartridge with 0.216ml to 26.0psi @05:42:08.57 Isolated @34.1psia (20.2psi) @05:42:08.61 Verifying cartridge holds at least 0.65..0.85 of 34.1psia @05:42:08.67 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @05:42:13.29 Cartridge pressure fell 2% to 33.5psia @05:42:13.43 Sampling 1000.0ml @05:42:13.44 SamplePump.setPosition! 0ml @05:42:13.54 Exhaust.open @05:42:14.32 Intake.open @05:42:15.20 Pressure.in :default @05:42:15.30 Pressure.hold @05:42:15.47 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @05:42:15.68 SamplePump.in :sample @05:42:15.78 SamplePump.seek 1000ml,1:23:51 @05:42:18.81 Cartridge 45 filtering 1/1000ml at 0.56ml/s with 31.8psi vacuum @05:43:18.83 Cartridge 45 filtering 33/1000ml at 0.47ml/s with 26.2psi vacuum @05:44:18.81 Cartridge 45 filtering 62/1000ml at 0.50ml/s with 20.9psi vacuum @05:45:18.83 Cartridge 45 filtering 90/1000ml at 0.49ml/s with 23.9psi vacuum @05:46:18.83 Cartridge 45 filtering 117/1000ml at 0.42ml/s with 14.1psi vacuum @05:47:18.82 Cartridge 45 filtering 143/1000ml at 0.45ml/s with 27.9psi vacuum @05:48:18.82 Cartridge 45 filtering 168/1000ml at 0.42ml/s with 20.5psi vacuum 1.8m deep (17.3psia) @05:49:18.83 Cartridge 45 filtering 193/1000ml at 0.43ml/s with 26.2psi vacuum @05:50:18.82 Cartridge 45 filtering 216/1000ml at 0.40ml/s with 14.3psi vacuum @05:51:18.83 Cartridge 45 filtering 239/1000ml at 0.39ml/s with 20.4psi vacuum 2.6m deep (18.6psia) @05:52:18.82 Cartridge 45 filtering 260/1000ml at 0.33ml/s with 26.9psi vacuum @05:53:18.83 Cartridge 45 filtering 281/1000ml at 0.34ml/s with 29.0psi vacuum @05:53:48.38 Session Ended @05:54:18.82 Cartridge 45 filtering 302/1000ml at 0.33ml/s with 15.8psi vacuum 4.0m deep (20.6psia) @05:55:18.82 Cartridge 45 filtering 321/1000ml at 0.34ml/s with 13.8psi vacuum 1.8m deep (17.4psia) @05:56:18.83 Cartridge 45 filtering 340/1000ml at 0.29ml/s with 24.7psi vacuum @05:57:09.45 Session Begun @05:57:18.83 Cartridge 45 filtering 358/1000ml at 0.32ml/s with 13.9psi vacuum @05:57:22.42 -> showlog -20 @05:58:18.82 Cartridge 45 filtering 376/1000ml at 0.27ml/s with 22.7psi vacuum @05:59:18.83 Cartridge 45 filtering 393/1000ml at 0.25ml/s with 25.0psi vacuum @06:00:18.81 Cartridge 45 filtering 409/1000ml at 0.26ml/s with 26.4psi vacuum @06:01:18.82 Cartridge 45 filtering 424/1000ml at 0.23ml/s with 25.3psi vacuum @06:02:18.82 Cartridge 45 filtering 439/1000ml at 0.25ml/s with 31.2psi vacuum @06:03:18.82 Cartridge 45 filtering 454/1000ml at 0.24ml/s with 18.8psi vacuum @06:04:18.83 Cartridge 45 filtering 468/1000ml at 0.23ml/s with 18.3psi vacuum @06:05:18.82 Cartridge 45 filtering 481/1000ml at 0.23ml/s with 31.1psi vacuum @06:06:18.82 Cartridge 45 filtering 494/1000ml at 0.26ml/s with 17.1psi vacuum @06:07:18.82 Cartridge 45 filtering 507/1000ml at 0.22ml/s with 27.1psi vacuum @06:07:23.89 Flow averaged 0.34ml/s over 25:09 @06:07:23.97 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @06:07:24.58 Relieved excess vacuum in .7 seconds @06:07:24.64 SamplePump.seek 1000ml,41:28 @06:08:13.29 Flow averaged 0.21ml/s over 49 seconds @06:08:13.38 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @06:08:13.99 Relieved excess vacuum in .7 seconds @06:08:14.05 SamplePump.seek 1000ml,40:36 @06:08:18.85 Cartridge 45 filtering 520/1000ml at 0.20ml/s with 24.3psi vacuum @06:08:41.50 Flow averaged 0.21ml/s over 28 seconds @06:08:41.57 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @06:08:42.68 Relieved excess vacuum in 1.2 seconds @06:08:42.74 SamplePump.seek 1000ml,40:06 @06:09:07.44 Flow averaged 0.21ml/s over 25 seconds @06:09:07.51 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @06:09:08.12 Relieved excess vacuum in .7 seconds @06:09:08.17 SamplePump.seek 1000ml,39:40 @06:09:18.84 Cartridge 45 filtering 532/1000ml at 0.22ml/s with 16.5psi vacuum @06:09:25.78 Flow averaged 0.21ml/s over 18 seconds @06:09:25.86 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @06:09:26.47 Relieved excess vacuum in .7 seconds @06:09:26.53 SamplePump.seek 1000ml,39:22 @06:09:41.21 Flow averaged 0.21ml/s over 15 seconds @06:09:41.28 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi @06:09:41.91 Relieved excess vacuum in .7 seconds @06:09:41.96 SamplePump.seek 1000ml,39:06 @06:09:53.72 Flow averaged 0.21ml/s over 12 seconds @06:09:53.78 Waiting up to 11 seconds for vacuum to reduce from 27.4 to 18.0psi @06:09:54.40 Relieved excess vacuum in .7 seconds @06:09:54.44 SamplePump.seek 1000ml,38:54 @06:10:03.47 Flow averaged 0.21ml/s over 10 seconds @06:10:03.55 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @06:10:04.17 Relieved excess vacuum in .7 seconds @06:10:04.23 SamplePump.seek 1000ml,38:45 @06:10:10.51 Flow estimate reduced to 0.16ml/s @06:10:10.54 Intake.close @06:10:10.60 Exhaust.close @06:10:10.95 Toroid.in :hold @06:10:11.05 Toroid.seek :bypass @06:10:12.73 Toroid.in :default @06:10:12.84 Toroid.seek :clear @06:10:14.49 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:10:14.52 Pressure.stop @06:10:14.81 Sampled 542.5ml @06:10:15.58 Plunge.seek :reagent @06:10:16.42 Sample loop closed. Ready to Deliver Reagents @06:10:16.45 Archive.seek :sealed @06:10:18.01 Gate.power :sampler,:OFF @06:10:20.43 Archive.seek :evac @06:10:35.06 delay 1:00 @06:11:35.10 Archive.seek :clear @06:11:52.61 RNAlater soak for 10:00 @06:16:58.19 Session Begun @06:17:10.19 -> showlog -50 @06:17:22.78 Session Ended @06:20:12.84 -> showlog -50 @06:21:52.65 Archive.seek :tVent @06:21:55.13 delay 1 second @06:21:56.17 Archive.seek :evac @06:22:11.71 delay 10 seconds @06:22:21.74 Archive.seek :tVent @06:22:37.28 delay 1 second @06:22:38.31 Archive.seek :evac @06:22:53.85 delay 10 seconds @06:23:03.89 Sample preserved and evacuated @06:23:04.04 Selecting Cartridge 44 @06:23:04.36 Carousel.in :default @06:23:04.53 Carousel.home.seek 45 @06:23:04.91 Archive.in :unlocking @06:23:05.08 Archive.seek :clear @06:23:05.24 Air.in :unlocking @06:23:05.41 Air.seek :clear @06:23:09.61 Lysis1.in :unlocking @06:23:09.82 Lysis1.seek :clear @06:23:09.86 Plunge.in :unlocking @06:23:10.02 Plunge.seek :clear @06:23:11.45 Carousel.seek 44 @06:23:12.02 Lysis1.in :fast @06:23:12.14 Lysis1.seek :locked @06:23:14.81 Plunge.in :default @06:23:14.91 Plunge.seek :locked @06:23:15.12 Air.in :fast @06:23:15.21 Air.seek :locked @06:23:16.81 Carousel.reconfigure :locked @06:23:17.00 Powering cartridge bus @06:23:19.67 Gate.setIR 1750,5000,0,6 @06:23:19.81 Gate.setIR 0,5000,0,6 @06:23:19.90 PuckHeater.configure :default #ambient 25.3C @06:23:19.99 Gate.power :heat,:ON,limit to 5000mA @06:23:20.70 Mounted lysis Cartridge board#Q05038 @06:23:20.75 Using default cartridge heat loss curve for HeatingAccelInCarousel @06:23:21.61 Preparing to filter cartridge 44 @06:23:21.89 Gate.power :sampler,:ON @06:23:22.02 Priming sample loop w/150ml, bypass w/50ml @06:23:22.04 SamplePump.setPosition! 0ml @06:23:22.14 Bypass.open @06:23:23.69 Intake.open @06:23:24.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:23:27.03 Vacuum tare is 56% of full scale @06:23:27.24 Pressure.in :default @06:23:27.33 Pressure.hold @06:23:27.59 Waiting for external sampler to finish priming... @06:23:27.61 delayUntil "06:26:51.91" @06:26:51.97 SamplePump.in :prime @06:26:52.07 SamplePump.seek 50ml,2:53 @06:27:53.11 Pressure.stop @06:27:53.19 SamplePump.setPosition! 0ml @06:27:53.28 Exhaust.open @06:27:54.72 Bypass.close @06:27:56.34 Pressure.in :default @06:27:56.44 Pressure.hold @06:27:56.79 SamplePump.in :prime @06:27:56.89 SamplePump.seek 150ml,7:39 @06:30:44.33 -> Can Can@06:30:32, 27.2C, 40% humidity, 14.4psia, 14.810V, 0.996A, 3.983Ah, 14.75W @06:30:59.08 Pressure.stop @06:30:59.20 Bypass.open @06:30:59.95 Intake.close @06:31:00.77 Toroid.in :hold @06:31:00.87 Toroid.seek :crack @06:31:02.45 Toroid.seek :cartridge @06:31:03.60 Toroid.in :default @06:31:03.69 Toroid.seek :clear @06:31:05.76 Pressurizing cartridge to 26psi above 14.1psia @06:31:05.78 SamplePump.setPosition! 0ml @06:31:05.88 Intake.open @06:31:06.56 Bypass.close @06:31:07.26 Exhaust.close @06:31:08.15 Pressure.in :default @06:31:08.24 Pressure.hold @06:31:09.05 SamplePump.in :charge @06:31:09.16 SamplePump.seek 2.3ml,42 seconds @06:31:22.55 Pressure.stop @06:31:22.64 Intake.close @06:31:23.41 Pressurized cartridge with 0.809ml to 26.0psi @06:31:23.43 Isolated @34.1psia (19.9psi) @06:31:23.45 Verifying cartridge holds at least 0.65..0.85 of 34.1psia @06:31:23.52 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:31:28.22 Sampling 50.0ml @06:31:28.24 SamplePump.setPosition! 0ml @06:31:28.38 Exhaust.open @06:31:29.14 Intake.open @06:31:30.00 Pressure.in :default @06:31:30.09 Pressure.hold @06:31:30.28 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @06:31:30.52 SamplePump.in :sample @06:31:30.63 SamplePump.seek 50ml,4:41 @06:31:33.65 Cartridge 44 filtering 1/50ml at 0.58ml/s with 17.7psi vacuum 5.4m deep (22.7psia) @06:32:33.65 Cartridge 44 filtering 32/50ml at 0.48ml/s with 24.9psi vacuum @06:33:11.11 Pressure.stop @06:33:11.29 Intake.close @06:33:11.37 Exhaust.close @06:33:11.63 Toroid.in :hold @06:33:11.72 Toroid.seek :bypass @06:33:13.38 Toroid.in :default @06:33:13.48 Toroid.seek :clear @06:33:15.12 Sampled 50.0ml @06:33:16.84 SPR.command "read_sequences" @06:33:16.92 SPR.command "stats_on" @06:33:17.80 SPR.command "start 0 1" @06:33:18.29 Gate.power :sampler,:OFF @06:33:18.50 SPR.start /var/log/esp/spr23aug0906h.spr.bz2 @06:33:20.55 Handoff.select :waste @06:33:27.30 Syringe tare is 17% of full scale @06:33:27.33 PinPort.in :locking @06:33:27.44 PinPort.seek :locked @06:33:30.00 Archive.in :fast @06:33:30.07 Lysis2.in :fast @06:33:30.18 Lysis1.seek 1350uL @06:33:30.36 Archive.seek :full @06:33:30.46 Lysis2.seek 950uL @06:33:35.27 Plunge.seek :reagent @06:33:36.27 Evacuating puck with filter bubble point of 50psi @06:33:36.34 PinPort.seek :locked @06:33:36.46 Handoff.select :waste @06:33:43.08 Air syringe pumping up to 1 strokes to reach 8psi @06:33:43.09 Air stroke #1 of 1 @06:33:43.24 Air.in :pressurize @06:33:43.33 Air.seek :pressurized @06:33:49.11 0.5psi after pressurize @06:33:49.13 Air.in :compress @06:33:49.25 Air.seek :evac @06:33:52.39 1.3psi after compress @06:34:03.49 0.1psi after compress (stable) @06:34:03.50 Air.in :default @06:34:03.67 Air.hold @06:34:03.93 at 0.2psi Opened to waste @06:34:03.95 Handoff.select :blocked @06:34:04.13 Archive syringe pumping up to 8 strokes to reach 50psi @06:34:04.14 Archive stroke #1 of 8 @06:34:04.30 Archive.in :pressurize @06:34:04.40 Archive.seek :pressurized @06:34:16.93 6.5psi after pressurize @06:34:22.57 6.4psi after pressurize (stable) @06:34:22.60 Archive.in :compress @06:34:22.72 Archive.seek :empty @06:34:37.91 20.7psi after compress @06:34:52.54 25.0psi after compress (stable) @06:34:52.56 Archive.in :gulp @06:34:52.68 Archive.seek :topVent @06:34:58.98 25.6psi after gulp @06:35:04.60 25.5psi after gulp (stable) @06:35:04.61 Archive stroke #2 of 8 @06:35:04.76 Archive.in :pressurize @06:35:04.85 Archive.seek :pressurized @06:35:17.42 24.8psi after pressurize @06:35:23.04 25.1psi after pressurize (stable) @06:35:23.09 Archive.in :compress @06:35:23.21 Archive.seek :empty @06:35:37.86 39.7psi after compress @06:35:50.89 43.5psi after compress (stable) @06:35:51.04 Archive.in :gulp @06:35:51.17 Archive.seek :topVent @06:35:57.45 44.0psi after gulp @06:36:03.06 43.8psi after gulp (stable) @06:36:03.07 Archive stroke #3 of 8 @06:36:03.22 Archive.in :pressurize @06:36:03.32 Archive.seek :pressurized @06:36:15.86 43.0psi after pressurize @06:36:21.96 43.3psi after pressurize (stable) @06:36:21.99 Archive.in :compress @06:36:22.11 Archive.seek :empty @06:36:33.33 Reached 50.2 of 50.0psi after pumping 5115uL (2.96 strokes) @06:36:33.35 Archive.in :default @06:36:33.47 Archive.hold @06:36:33.57 Handoff.select :waste @06:36:43.32 delay 1:00 @06:37:43.34 Handoff.select :blocked @06:38:06.08 Handoff.select :waste @06:38:06.24 0.1psi at evacEnd @06:38:06.26 Handoff.select :blocked @06:38:06.46 Archive.in :fast @06:38:06.55 Archive.seek :topVent @06:38:20.01 Stable @0.2psi after Handoff blocked @06:38:20.06 Archive syringe pumping up to 8 strokes to reach 30psi @06:38:20.08 Archive stroke #1 of 8 @06:38:20.21 Archive.in :pressurize @06:38:20.35 Archive.seek :pressurized @06:38:32.88 1.0psi after pressurize @06:38:32.91 Archive.in :compress @06:38:33.02 Archive.seek :empty @06:38:47.66 14.1psi after compress @06:38:58.29 15.1psi after compress (stable) @06:38:58.32 Archive.in :gulp @06:38:58.49 Archive.seek :topVent @06:39:04.78 15.2psi after gulp @06:39:10.38 15.2psi after gulp (stable) @06:39:10.39 Archive stroke #2 of 8 @06:39:10.53 Archive.in :pressurize @06:39:10.63 Archive.seek :pressurized @06:39:23.15 15.2psi after pressurize @06:39:23.18 Archive.in :compress @06:39:23.34 Archive.seek :empty @06:39:38.01 28.1psi after compress @06:39:39.09 Reached 28.1 of 30.2psi after pumping 3460uL (2.00 strokes) @06:39:39.12 Archive.in :default @06:39:39.37 Archive.hold @06:39:39.51 Handoff.select :waste @06:39:46.81 delay 1:00 @06:40:46.84 Handoff.select :blocked @06:41:09.57 Handoff.select :waste @06:41:09.73 0.2psi at evacEnd @06:41:09.75 Air.seek :fullVent @06:41:11.22 Archive.in :fast @06:41:11.32 Archive.seek :topVent @06:41:18.44 Seawater evacuated, Pressure above/below filter released. @06:41:18.57 Lysis1.in :slow @06:41:18.69 Lysis1.seek 1050uL @06:41:48.72 delay 5 seconds @06:41:53.74 Handoff.select :blocked @06:41:53.97 Lysis1.seek 450uL @06:42:53.89 delay 3 seconds @06:42:57.00 Air.in mix with 1.40048 parts Lysis1 @06:42:57.71 Air.seek :evac @06:42:57.80 Lysis1.seek 300uL @06:43:12.93 Lysis1.seek :empty @06:43:43.05 Lysis buffer delivered @52.2psi @06:43:43.08 Polling Can every 2:00 @06:43:43.64 PuckHeater.configure :default #ambient 27.4C @06:43:43.72 PuckHeater.seek 79.5C @06:44:35.70 PuckHeater.hold 2:00,79.5C @06:46:20.33 Session Ended @06:46:35.35 Polling Can every 2:00 @06:46:35.90 PuckHeater.hold 30 seconds,78.5C @06:46:54.61 Session Begun @06:47:06.02 Polling Can every 2:00 @06:47:06.60 PuckHeater.configure :default #ambient 27.4C @06:47:06.69 PuckHeater.hold 30 seconds,78C @06:47:16.59 -> showlog -20 @06:47:36.85 Polling Can every 2:00 @06:47:37.41 PuckHeater.configure :default #ambient 27.3C @06:47:37.50 PuckHeater.hold 10:00,77.5C @06:55:21.17 -> showlog -20 @06:57:35.70 Lysis complete. Syringe pressure @49.8psi @06:57:35.75 Archive syringe pumping up to 5 strokes to reach 40psi @06:57:35.80 Archive stroke #1 of 5 @06:57:35.93 Archive.in :pressurize @06:57:36.03 Archive.seek :pressurized @06:57:36.31 Reached 49.9 of 40.0psi after pumping 1uL (0.00 strokes) @06:57:36.33 Archive.in :default @06:57:36.44 Archive.hold @06:57:36.55 delay 2 seconds @06:57:38.58 Archive.in :fast @06:57:38.68 Archive.seek :full @06:57:38.96 Lysis1.seek 138uL @06:57:52.86 delay 1 second @06:57:53.89 Archive.seek :full @06:57:53.96 Lysis1.seek :empty @06:58:07.92 Handoff.select :blocked @06:58:08.11 Initial air pressure @48.9psi @06:58:08.22 Lysis1.in :extract @06:58:08.38 Lysis1.seek 600uL,nil @06:58:12.17 Archive syringe pumping up to 6 strokes to reach 25psi @06:58:12.18 Archive stroke #1 of 6 @06:58:12.43 Archive.in :pressurize @06:58:12.55 Archive.seek :pressurized @06:58:25.15 11.9psi after pressurize @06:58:30.77 11.8psi after pressurize (stable) @06:58:30.79 Archive.in :compress @06:58:30.93 Archive.seek :empty @06:58:39.72 Archive.in :default @06:58:39.80 Archive.hold @06:58:40.01 Initial 600uL lysate @19.3psi @06:58:40.04 Handoff.select :blocked @06:58:40.21 Initial air pressure @19.9psi @06:58:40.35 Lysis1.seek 825uL,nil @06:58:42.89 Recovered 825uL lysate @15.0psi @06:58:42.95 Air.in :halfSpeed @06:58:43.06 Air.seek :fullVent @06:58:45.31 Puck Top Vented, bottom @16.3psi @06:58:45.33 Handoff.select :waste @06:58:45.50 delay 5 seconds @06:58:50.59 Handoff open to waste, bottom @1.0psi @06:58:50.62 Air.in :default @06:58:50.78 Air.seek :evac @06:58:52.23 Lysis2.in :DAmix @06:58:52.46 Lysis1.in :DAmix @06:58:53.07 Lysis2.seek 700uL @06:58:53.18 Lysis1.seek 575uL @06:59:28.24 delay 5 seconds @06:59:33.31 Handoff.select SPR @06:59:33.48 SPR.command "start 2 14" @06:59:34.93 SPR stabilized @06:59:35.13 delay 10 seconds @06:59:45.15 SPR.command "start 2 0" @06:59:47.77 Waiting up to 11:00 for SPR to enter state 1 @07:00:36.24 -> showlog -20 @07:00:51.25 -> showlog -20 @07:01:01.85 -> showlog -20 @07:01:45.45 SPR.command "hold_on" @07:01:46.84 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:01:47.53 Lysis2.seek 400uL @07:01:47.58 Lysis1.seek 275uL @07:02:29.66 delay 15 seconds @07:02:44.69 SPR.command "hold_off" @07:02:47.48 Waiting up to 38:00 for SPR to enter state 7 @07:14:07.17 -> showlog -20 @07:16:42.46 Session Begun @07:16:59.55 -> showlog -20 @07:17:07.68 SPRsummary:2.19e+09RIU,4e+09RIU,5.33e+08RIU,132RIU,none,high,high after 2777s @07:21:11.50 -> showlog -20 @07:24:08.15 Session Ended @07:25:53.41 -> showlog -20 @07:26:04.93 -> showlog -20 @07:26:22.86 -> Can Can@07:26:22, 27.0C, 39% humidity, 14.4psia, 14.853V, 0.534A, 4.690Ah, 7.93W @07:27:41.25 -> showlog -20 @07:28:38.11 SPR analysis completed @07:28:44.96 Ended log spr23aug0906h.spr.bz2 @07:28:44.98 SPR.command "stats_off" @07:28:45.36 Handoff.select :close @07:28:45.54 Gate.power :heat,:OFF @07:28:45.56 Gate.power :cartridge,:OFF @07:28:45.69 PinPort.in :unlocking @07:28:45.78 PinPort.seek :clear @07:28:47.73 Carousel.in :default @07:28:47.89 Carousel.home.seek 44 @07:28:48.30 Plunge.seek :locked @07:28:49.23 Archive.in :unlocking @07:28:49.41 Archive.seek :clear @07:28:49.51 Air.in :unlocking @07:28:49.71 Air.seek :clear @07:28:49.76 Lysis1.in :unlocking @07:28:49.98 Lysis1.seek :clear @07:28:50.11 Lysis2.in :unlocking @07:28:50.22 Lysis2.seek :clear @07:28:54.15 Gate.power :motor,:OFF @07:28:54.18 Gate.power :core,:OFF @07:28:54.21 Gate.power :analytic1,:OFF @07:28:54.27 Gate.power :analytic2,:OFF @07:28:54.31 Gate.power :main,:OFF @07:28:54.34 Safely stopped and ready to power off @07:28:56.77 -> slots 44 {44 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0906h.spr.bz2}} @07:30:49.36 -> start {fire :dry, dry} # @07:30:49.59 Gate.power :sampler,:OFF,limit to 3500mA @07:30:49.62 Gate.power :main,:ON,limit to 5000mA @07:30:49.66 Gate.power :analytic1,:ON,limit to 5000mA @07:30:49.69 Gate.power :analytic2,:ON,limit to 5000mA @07:30:49.80 Powering core bus @07:30:52.15 SPR Module 1, FW Rev. B, FPGA Rev. C @07:30:52.58 Archive.configure :default @07:30:52.70 Air.configure :default @07:30:52.79 Lysis1.configure :default @07:30:52.89 Lysis2.configure :default @07:30:53.01 Plunge.configure :default @07:30:53.13 SamplePump.configure :default @07:30:53.26 Toroid.configure :default @07:30:53.38 Carousel.configure :default @07:30:53.50 Pressure.configure :default @07:30:53.62 ExtraPump.configure :default @07:30:53.71 PinPort.configure :default @07:30:53.81 All elf controllers running firmware version 4.84 @07:30:53.83 Gate.power :motor,:ON,limit to 5000mA @07:30:54.16 Archive.home.jog 0 @07:30:54.98 PinPort.home.jog 0 @07:30:55.03 Toroid.home.jog 0 @07:30:55.09 Lysis2.home.jog 0 @07:30:55.15 Lysis1.home.jog 0 @07:30:55.19 Plunge.home.jog 0 @07:30:55.24 Air.home.jog 0 @07:30:55.28 Carousel.home.jog 0 @07:30:56.04 Archive.in :homing @07:30:56.58 Archive.jog 1387 @07:30:56.66 Toroid.in :homing @07:30:56.72 Lysis2.in :homing @07:30:56.76 Air.in :homing @07:30:56.81 PinPort.in :homing @07:30:56.88 Plunge.in :homing @07:30:56.92 Lysis1.in :homing @07:30:57.27 Toroid.jog 610 @07:30:57.36 Lysis2.jog 1390 @07:30:57.44 Air.jog 1369 @07:30:57.49 PinPort.jog -107 @07:30:57.53 Plunge.jog 1571 @07:30:57.57 Lysis1.jog 1383 @07:30:58.03 PinPort.setRawPosition! 0 @07:30:58.12 PinPort.seek :home @07:30:58.19 Archive.setRawPosition! 50 @07:30:58.39 Archive.seek :home @07:30:58.51 PinPort.in :default @07:30:58.70 Archive.in :default @07:30:58.87 Lysis2.setRawPosition! 50 @07:30:58.94 Lysis2.seek :home @07:30:59.06 Air.setRawPosition! 50 @07:30:59.15 Air.seek :home @07:30:59.31 Lysis2.in :default @07:30:59.34 Lysis1.setRawPosition! 50 @07:30:59.88 Plunge.setRawPosition! 50 @07:31:00.09 Lysis1.seek :home @07:31:00.12 Plunge.seek :home @07:31:00.40 Air.in :default @07:31:00.43 Toroid.setRawPosition! 0 @07:31:00.60 Toroid.seek :home @07:31:00.76 Toroid.in :default @07:31:00.79 Plunge.in :default @07:31:00.83 Lysis1.in :default @07:31:01.32 Toroid.seek :clear @07:31:02.96 Carousel.in :default @07:31:03.13 Carousel.home.seek 44 @07:31:03.44 Selecting Cartridge 43 @07:31:03.78 Carousel.seek 43 @07:31:04.39 Lysis1.in :fast @07:31:04.49 Lysis1.seek :locked @07:31:07.16 Plunge.seek :locked @07:31:07.36 Air.in :fast @07:31:07.45 Air.seek :locked @07:31:09.05 Carousel.reconfigure :locked @07:31:09.26 Powering cartridge bus @07:31:11.93 Gate.setIR 1750,5000,0,6 @07:31:12.06 Gate.setIR 0,5000,0,6 @07:31:12.18 Gate.power :cartridge,:OFF @07:31:12.75 Mounted archive Cartridge @07:31:13.12 Preparing to filter cartridge 43 @07:31:13.40 Gate.power :sampler,:ON @07:31:13.53 Priming sample loop w/150ml, bypass w/50ml @07:31:13.54 SamplePump.setPosition! 0ml @07:31:13.63 Bypass.open @07:31:15.15 Intake.open @07:31:15.94 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:31:18.53 Vacuum tare is 56% of full scale @07:31:18.73 Pressure.in :default @07:31:18.81 Pressure.hold @07:31:19.08 Waiting for external sampler to finish priming... @07:31:19.10 delayUntil "07:34:43.42" @07:34:43.48 SamplePump.in :prime @07:34:43.58 SamplePump.seek 50ml,2:53 @07:35:44.62 Pressure.stop @07:35:44.70 SamplePump.setPosition! 0ml @07:35:44.78 Exhaust.open @07:35:46.28 Bypass.close @07:35:47.88 Pressure.in :default @07:35:47.98 Pressure.hold @07:35:48.37 SamplePump.in :prime @07:35:48.48 SamplePump.seek 150ml,7:39 @07:38:50.86 Pressure.stop @07:38:50.98 Bypass.open @07:38:51.73 Intake.close @07:38:52.52 Toroid.in :hold @07:38:52.62 Toroid.seek :crack @07:38:54.19 Toroid.seek :cartridge @07:38:55.37 Toroid.in :default @07:38:55.47 Toroid.seek :clear @07:38:57.53 Pressurizing cartridge to 26psi above 13.6psia @07:38:57.55 SamplePump.setPosition! 0ml @07:38:57.63 Intake.open @07:38:58.31 Bypass.close @07:38:59.02 Exhaust.close @07:38:59.88 Pressure.in :default @07:38:59.98 Pressure.hold @07:39:00.29 SamplePump.in :charge @07:39:00.40 SamplePump.seek 2.3ml,42 seconds @07:39:07.68 Pressure.stop @07:39:07.77 Intake.close @07:39:08.55 Pressurized cartridge with 0.233ml to 26.1psi @07:39:08.57 Isolated @34.2psia (20.6psi) @07:39:08.61 Verifying cartridge holds at least 0.65..0.85 of 34.2psia @07:39:08.67 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:39:13.29 Cartridge pressure fell 1% to 33.9psia @07:39:13.42 Sampling 1000.0ml @07:39:13.44 SamplePump.setPosition! 0ml @07:39:13.53 Exhaust.open @07:39:14.29 Intake.open @07:39:15.65 Pressure.in :default @07:39:15.75 Pressure.hold @07:39:15.92 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:39:16.15 SamplePump.in :sample @07:39:16.28 SamplePump.seek 1000ml,1:23:51 @07:39:19.29 Cartridge 43 filtering 1/1000ml at 0.54ml/s with 26.7psi vacuum @07:40:19.29 Cartridge 43 filtering 33/1000ml at 0.53ml/s with 18.7psi vacuum 2.7m deep (18.6psia) @07:41:19.30 Cartridge 43 filtering 61/1000ml at 0.48ml/s with 16.1psi vacuum 1.9m deep (17.5psia) @07:42:19.32 Cartridge 43 filtering 88/1000ml at 0.40ml/s with 26.5psi vacuum @07:43:19.30 Cartridge 43 filtering 114/1000ml at 0.42ml/s with 30.0psi vacuum @07:44:19.28 Cartridge 43 filtering 138/1000ml at 0.42ml/s with 18.2psi vacuum 2.0m deep (17.6psia) @07:45:19.30 Cartridge 43 filtering 161/1000ml at 0.39ml/s with 14.8psi vacuum 2.7m deep (18.7psia) @07:46:19.29 Cartridge 43 filtering 183/1000ml at 0.37ml/s with 19.6psi vacuum 1.9m deep (17.5psia) @07:47:19.30 Cartridge 43 filtering 205/1000ml at 0.33ml/s with 13.0psi vacuum @07:48:19.29 Cartridge 43 filtering 225/1000ml at 0.37ml/s with 16.9psi vacuum 4.0m deep (20.6psia) @07:48:25.91 Session Begun @07:48:42.32 -> showlog -20 @07:49:19.30 Cartridge 43 filtering 245/1000ml at 0.33ml/s with 14.6psi vacuum @07:50:19.29 Cartridge 43 filtering 263/1000ml at 0.29ml/s with 26.5psi vacuum @07:50:42.60 -> showlog -5 @07:51:19.29 Cartridge 43 filtering 281/1000ml at 0.25ml/s with 28.7psi vacuum @07:52:19.28 Cartridge 43 filtering 298/1000ml at 0.24ml/s with 24.5psi vacuum @07:53:19.29 Cartridge 43 filtering 314/1000ml at 0.28ml/s with 28.2psi vacuum @07:53:42.60 -> Can Can@07:52:17, 26.8C, 39% humidity, 14.4psia, 14.826V, 0.827A, 4.994Ah, 12.26W @07:54:19.30 Cartridge 43 filtering 330/1000ml at 0.30ml/s with 30.2psi vacuum @07:55:19.34 Cartridge 43 filtering 345/1000ml at 0.23ml/s with 16.5psi vacuum @07:56:19.29 Cartridge 43 filtering 359/1000ml at 0.23ml/s with 14.9psi vacuum @07:57:19.41 Cartridge 43 filtering 373/1000ml at 0.21ml/s with 29.9psi vacuum @07:57:54.01 Session Ended @07:58:19.30 Cartridge 43 filtering 386/1000ml at 0.20ml/s with 25.0psi vacuum @07:58:34.66 -> showlog -5 @07:58:51.83 Flow averaged 0.33ml/s over 19:36 @07:58:51.91 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @07:58:52.51 Relieved excess vacuum in .7 seconds @07:58:52.57 SamplePump.seek 1000ml,51:04 @07:59:19.28 Cartridge 43 filtering 399/1000ml at 0.21ml/s with 31.0psi vacuum @07:59:43.66 Flow averaged 0.22ml/s over 52 seconds @07:59:43.73 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @07:59:44.75 Relieved excess vacuum in 1.1 seconds @07:59:44.81 SamplePump.seek 1000ml,50:07 @08:00:19.29 Cartridge 43 filtering 412/1000ml at 0.20ml/s with 27.3psi vacuum @08:00:33.30 Flow averaged 0.22ml/s over 49 seconds @08:00:33.38 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @08:00:33.98 Relieved excess vacuum in .7 seconds @08:00:34.02 SamplePump.seek 1000ml,49:13 @08:01:05.01 Flow averaged 0.22ml/s over 31 seconds @08:01:05.10 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @08:01:05.71 Relieved excess vacuum in .7 seconds @08:01:05.76 SamplePump.seek 1000ml,48:39 @08:01:19.28 Cartridge 43 filtering 425/1000ml at 0.23ml/s with 17.8psi vacuum 3.1m deep (19.3psia) @08:01:28.37 Flow averaged 0.22ml/s over 23 seconds @08:01:28.45 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @08:01:29.07 Relieved excess vacuum in .7 seconds @08:01:29.12 SamplePump.seek 1000ml,48:15 @08:01:44.76 Flow averaged 0.22ml/s over 16 seconds @08:01:44.84 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @08:01:45.45 Relieved excess vacuum in .7 seconds @08:01:45.49 SamplePump.seek 1000ml,47:58 @08:01:55.64 Flow averaged 0.21ml/s over 11 seconds @08:01:55.71 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @08:01:56.37 Relieved excess vacuum in .7 seconds @08:01:56.42 SamplePump.seek 1000ml,47:47 @08:02:08.00 Flow averaged 0.21ml/s over 12 seconds @08:02:08.09 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @08:02:08.69 Relieved excess vacuum in .7 seconds @08:02:08.73 SamplePump.seek 1000ml,47:35 @08:02:18.88 Flow averaged 0.21ml/s over 11 seconds @08:02:18.95 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @08:02:19.28 Cartridge 43 filtering 437/1000ml at 0.00ml/s with 3.6psi vacuum @08:02:19.56 Relieved excess vacuum in .7 seconds @08:02:19.60 SamplePump.seek 1000ml,47:24 @08:02:27.89 Flow estimate reduced to 0.17ml/s @08:02:27.92 Intake.close @08:02:28.02 Exhaust.close @08:02:28.38 Toroid.in :hold @08:02:28.49 Toroid.seek :bypass @08:02:30.18 Toroid.in :default @08:02:30.31 Toroid.seek :clear @08:02:32.58 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:02:32.61 Pressure.stop @08:02:32.88 Sampled 439.1ml @08:02:33.64 Plunge.seek :reagent @08:02:34.48 Sample loop closed. Ready to Deliver Reagents @08:02:34.50 Archive.seek :sealed @08:02:36.05 Gate.power :sampler,:OFF @08:02:38.47 Archive.seek :evac @08:02:52.56 delay 1:00 @08:03:52.60 Archive.seek :clear @08:04:10.12 RNAlater soak for 10:00 @08:04:14.20 Session Begun @08:04:33.95 -> showlog -10 @08:08:34.60 Session Ended @08:14:10.16 Archive.seek :tVent @08:14:12.64 delay 1 second @08:14:13.67 Archive.seek :evac @08:14:29.22 delay 10 seconds @08:14:39.26 Archive.seek :tVent @08:14:54.79 delay 1 second @08:14:55.82 Archive.seek :evac @08:15:11.37 delay 10 seconds @08:15:21.41 Sample preserved and evacuated @08:15:21.56 Selecting Cartridge 42 @08:15:21.83 Carousel.in :default @08:15:22.00 Carousel.home.seek 43 @08:15:22.41 Archive.in :unlocking @08:15:22.61 Archive.seek :clear @08:15:22.69 Air.in :unlocking @08:15:22.90 Air.seek :clear @08:15:27.28 Lysis1.in :unlocking @08:15:27.48 Lysis1.seek :clear @08:15:27.52 Plunge.in :unlocking @08:15:27.68 Plunge.seek :clear @08:15:29.12 Carousel.seek 42 @08:15:29.69 Lysis1.in :fast @08:15:29.79 Lysis1.seek :locked @08:15:32.45 Plunge.in :default @08:15:32.58 Plunge.seek :locked @08:15:32.74 Air.in :fast @08:15:32.83 Air.seek :locked @08:15:34.94 Carousel.reconfigure :locked @08:15:35.15 Powering cartridge bus @08:15:37.86 Gate.setIR 1750,5000,0,6 @08:15:38.00 Gate.setIR 0,5000,0,6 @08:15:38.10 PuckHeater.configure :default #ambient 27.3C @08:15:38.19 Gate.power :heat,:ON,limit to 5000mA @08:15:38.89 Mounted lysis Cartridge board#Q05064 @08:15:38.96 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:15:39.32 Preparing to filter cartridge 42 @08:15:39.60 Gate.power :sampler,:ON @08:15:39.71 Priming sample loop w/150ml, bypass w/50ml @08:15:39.74 SamplePump.setPosition! 0ml @08:15:39.83 Bypass.open @08:15:41.36 Intake.open @08:15:42.14 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:15:44.74 Vacuum tare is 56% of full scale @08:15:44.94 Pressure.in :default @08:15:45.03 Pressure.hold @08:15:45.30 Waiting for external sampler to finish priming... @08:15:45.31 delayUntil "08:19:09.61" @08:19:09.66 SamplePump.in :prime @08:19:09.77 SamplePump.seek 50ml,2:53 @08:20:10.81 Pressure.stop @08:20:10.95 SamplePump.setPosition! 0ml @08:20:11.03 Exhaust.open @08:20:12.50 Bypass.close @08:20:14.11 Pressure.in :default @08:20:14.20 Pressure.hold @08:20:14.59 SamplePump.in :prime @08:20:14.69 SamplePump.seek 150ml,7:39 @08:23:17.02 Pressure.stop @08:23:17.13 Bypass.open @08:23:17.92 Intake.close @08:23:18.72 Toroid.in :hold @08:23:18.82 Toroid.seek :crack @08:23:20.39 Toroid.seek :cartridge @08:23:21.93 Toroid.in :default @08:23:22.13 Toroid.seek :clear @08:23:24.19 Pressurizing cartridge to 26psi above 13.7psia @08:23:24.21 SamplePump.setPosition! 0ml @08:23:24.34 Intake.open @08:23:25.05 Bypass.close @08:23:25.74 Exhaust.close @08:23:26.63 Pressure.in :default @08:23:26.72 Pressure.hold @08:23:26.99 SamplePump.in :charge @08:23:27.11 SamplePump.seek 2.3ml,42 seconds @08:23:40.41 Pressure.stop @08:23:40.51 Intake.close @08:23:41.31 Pressurized cartridge with 0.799ml to 27.1psi @08:23:41.32 Isolated @33.1psia (19.4psi) @08:23:41.36 Verifying cartridge holds at least 0.65..0.85 of 33.1psia @08:23:41.42 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:23:46.04 Cartridge pressure fell 1% to 32.9psia @08:23:46.18 Sampling 50.0ml @08:23:46.19 SamplePump.setPosition! 0ml @08:23:46.33 Exhaust.open @08:23:47.05 Intake.open @08:23:47.91 Pressure.in :default @08:23:48.00 Pressure.hold @08:23:48.18 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:23:48.44 SamplePump.in :sample @08:23:48.54 SamplePump.seek 50ml,4:41 @08:23:51.57 Cartridge 42 filtering 1/50ml at 0.56ml/s with 26.8psi vacuum @08:24:51.58 Cartridge 42 filtering 33/50ml at 0.45ml/s with 20.0psi vacuum @08:25:26.85 Pressure.stop @08:25:27.04 Intake.close @08:25:27.14 Exhaust.close @08:25:27.37 Toroid.in :hold @08:25:27.47 Toroid.seek :bypass @08:25:29.14 Toroid.in :default @08:25:29.25 Toroid.seek :clear @08:25:30.90 Sampled 50.0ml @08:25:33.19 SPR.command "read_sequences" @08:25:33.28 SPR.command "stats_on" @08:25:34.16 SPR.command "start 0 1" @08:25:34.59 Gate.power :sampler,:OFF @08:25:34.85 SPR.start /var/log/esp/spr23aug0908h.spr.bz2 @08:25:36.90 Handoff.select :waste @08:25:43.63 Syringe tare is 18% of full scale @08:25:43.66 PinPort.in :locking @08:25:43.78 PinPort.seek :locked @08:25:46.35 Archive.in :fast @08:25:46.40 Lysis2.in :fast @08:25:46.54 Lysis1.seek 1350uL @08:25:46.65 Archive.seek :full @08:25:46.78 Lysis2.seek 950uL @08:25:51.63 Plunge.seek :reagent @08:25:52.63 Evacuating puck with filter bubble point of 50psi @08:25:52.66 PinPort.seek :locked @08:25:52.81 Handoff.select :waste @08:25:59.43 Air syringe pumping up to 1 strokes to reach 8psi @08:25:59.44 Air stroke #1 of 1 @08:25:59.58 Air.in :pressurize @08:26:00.13 Air.seek :pressurized @08:26:06.03 0.8psi after pressurize @08:26:06.07 Air.in :compress @08:26:06.18 Air.seek :evac @08:26:09.23 0.9psi after compress @08:26:19.84 0.2psi after compress (stable) @08:26:19.86 Air.in :default @08:26:20.00 Air.hold @08:26:20.32 at 0.1psi Opened to waste @08:26:20.34 Handoff.select :blocked @08:26:20.52 Archive syringe pumping up to 8 strokes to reach 50psi @08:26:20.54 Archive stroke #1 of 8 @08:26:20.67 Archive.in :pressurize @08:26:20.77 Archive.seek :pressurized @08:26:33.31 8.0psi after pressurize @08:26:45.41 10.1psi after pressurize (stable) @08:26:45.44 Archive.in :compress @08:26:45.59 Archive.seek :empty @08:27:00.28 23.9psi after compress @08:27:20.89 31.3psi after compress (stable) @08:27:20.92 Archive.in :gulp @08:27:21.03 Archive.seek :topVent @08:27:27.32 31.8psi after gulp @08:27:32.92 31.4psi after gulp (stable) @08:27:32.94 Archive stroke #2 of 8 @08:27:33.09 Archive.in :pressurize @08:27:33.18 Archive.seek :pressurized @08:27:45.72 30.1psi after pressurize @08:27:52.36 30.8psi after pressurize (stable) @08:27:52.39 Archive.in :compress @08:27:52.50 Archive.seek :empty @08:28:07.16 45.7psi after compress @08:30:42.61 Session Begun @08:30:54.20 -> showlog -20 @08:31:46.34 -> showlog -50 @08:35:51.97 -> showlog -10 @08:36:35.32 -> clients [ESP["ben#2"],ESP["ben"]] @08:37:57.94 Session Ended @08:38:52.97 -> slots 43 {43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}} @08:39:04.81 -> slots 42 {42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug0908h.spr.bz2}} @08:45:51.52 -> clients [ESP["ben"]] @08:45:59.05 -> showlog -10 @08:46:46.09 -> slots 42 {42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug0908h.spr.bz2}} @08:54:32.81 -> exit @08:54:32.84 Session Ended @08:55:01.66 Session Begun @08:55:14.81 -> showlog -10 @08:55:40.37 -> clients [ESP["ben"]] @09:15:23.96 -> Can Can@08:28:10, 27.1C, 38% humidity, 14.4psia, 14.858V, 0.482A, 5.403Ah, 7.16W @09:36:50.45 Session Begun @09:36:52.46 -> clients [ESP["ben"],ESP["brent"]] @09:37:13.00 -> list method :startDoublet @09:37:13.06 Errno::ENOENT in brent -- Missing ruby source: brent:3 @09:37:33.41 -> showlog 30 @09:38:20.76 -> #Better to specify the cartridge types explicitly than :dry, :dry @09:38:50.52 -> #The new "startDoublet" command makes this easy @09:40:00.92 -> #startDoublet is start {fire :archiveHiBiomass_bac, :microSPR_phyto} @09:40:41.89 -> #guarentees that the 1st cart is an archive, followed by an SPR @09:41:18.43 Session Begun @09:41:34.69 -> showlog 10 @09:41:58.69 -> showlog -20 @09:42:06.28 Session Ended @09:42:19.82 -> showlog -100 @09:44:27.80 -> # I will use that for the next sample, thanks @09:44:47.01 -> # it looks like the previous two worked as planned @09:46:57.56 -> Can Can@08:28:10, 27.1C, 38% humidity, 14.4psia, 14.858V, 0.482A, 5.403Ah, 7.16W @12:15:59.10PDT09-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @12:16:02.77 >BREAK< ESP3 Gateway boot v1.43 for core bus @12:16:02.80 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @12:16:06.30 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @12:16:06.92 Sea pressure (Abs) tare is 6% of full scale @12:16:15.78 Polling Can every 2:00 @12:16:15.83 Waiting for espclients... @12:16:16.34 Session Begun @12:16:21.25 -> Can Can@12:16:16, 25.1C, 44% humidity, 14.3psia, 14.884V, 0.088A, 0.008Ah, 1.31W @12:17:14.55 -> exit @12:17:14.57 Session Ended @12:45:59.33 Session Begun @12:46:05.95 Session Begun @12:46:22.81 -> #hi Ben. I'm Chris @12:47:28.04 -> refillSampleLines :refillSampleLines @12:48:18.24 -> #run >require 'vacuumCart' @12:48:34.49 -> #then retry the command. @12:50:06.31 -> require 'vacuumCart' true @12:50:19.51 -> refillSampleLines @12:50:19.54 Refilling sample & bypass lines with fluid. @12:50:19.64 Gate.power :sampler,:OFF,limit to 3500mA @12:50:19.68 Gate.power :main,:ON,limit to 5000mA @12:50:19.71 Gate.power :analytic1,:ON,limit to 5000mA @12:50:19.76 Gate.power :analytic2,:ON,limit to 5000mA @12:50:19.97 Powering core bus @12:50:22.23 SPR Module 1, FW Rev. B, FPGA Rev. C @12:50:22.74 Archive.configure :default @12:50:22.88 Air.configure :default @12:50:22.98 Lysis1.configure :default @12:50:23.09 Lysis2.configure :default @12:50:23.23 Plunge.configure :default @12:50:23.36 SamplePump.configure :default @12:50:23.50 Toroid.configure :default @12:50:23.63 Carousel.configure :default @12:50:23.76 Pressure.configure :default @12:50:23.91 ExtraPump.configure :default @12:50:24.00 PinPort.configure :default @12:50:24.12 All elf controllers running firmware version 4.84 @12:50:24.21 Gate.power :motor,:ON,limit to 5000mA @12:50:24.98 Archive.home.jog 0 @12:50:25.82 Carousel.home.jog 0 @12:50:25.87 Toroid.home.jog 0 @12:50:25.93 Lysis2.home.jog 0 @12:50:25.99 Air.home.jog 0 @12:50:26.06 PinPort.home.jog 0 @12:50:26.09 Lysis1.home.jog 0 @12:50:26.15 Plunge.home.jog 0 @12:50:27.07 Archive.in :homing @12:50:27.36 Archive.jog 9711 @12:50:27.44 PinPort.in :homing @12:50:27.48 Plunge.in :homing @12:50:27.55 Lysis1.in :homing @12:50:27.63 Toroid.in :homing @12:50:27.69 Air.in :homing @12:50:27.75 Lysis2.in :homing @12:50:28.21 PinPort.jog -2235 @12:50:28.23 Plunge.jog 2301 @12:50:28.31 Lysis1.jog 3133 @12:50:28.38 Toroid.jog 604 @12:50:28.43 Air.jog 8970 @12:50:28.50 Lysis2.jog 5158 @12:50:30.24 Plunge.setRawPosition! 50 @12:50:30.34 Plunge.seek :home @12:50:30.67 Lysis1.setRawPosition! 50 @12:50:30.70 Plunge.in :default @12:50:30.87 Lysis1.seek :home @12:50:31.01 Toroid.setRawPosition! 0 @12:50:31.11 Toroid.seek :home @12:50:31.28 Lysis1.in :default @12:50:31.31 Toroid.in :default @12:50:31.71 PinPort.setRawPosition! 0 @12:50:31.81 PinPort.seek :home @12:50:31.95 Lysis2.setRawPosition! 50 @12:50:32.08 PinPort.in :default @12:50:32.11 Lysis2.seek :home @12:50:32.52 Lysis2.in :default @12:50:32.93 Archive.setRawPosition! 50 @12:50:33.02 Archive.seek :home @12:50:33.34 Archive.in :default @12:50:33.48 Air.setRawPosition! 50 @12:50:33.57 Air.seek :home @12:50:33.89 Air.in :default @12:50:34.26 Toroid.seek :clear @12:50:35.92 Carousel.in :default @12:50:36.10 Carousel.home.seek 42 @12:50:36.47 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @12:51:04.59 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @12:51:04.61 Bypass.open @12:51:05.39 Intake.open @12:51:06.11 SamplePump.setPosition! 0ml @12:51:06.30 SamplePump.seek 30ml @12:51:36.99 -> SP.rate 0.822470663019764 @12:51:39.71 -> SP.rate 0.822470663019764 @12:51:43.18 Bypass.close @12:51:43.99 Exhaust.open @12:51:44.84 SamplePump.seek 55ml @12:52:01.01 -> SP.rate 0.822470663019764 @12:52:15.67 delay 1 second @12:52:16.70 Intake.close @12:52:16.77 Exhaust.close @12:52:18.17 Sampling Loop filled with water @13:01:05.51 Session Ended @13:01:24.18 Session Begun @13:02:14.43 -> BACsampler.prime @13:02:14.57 Priming sample loop w/150ml, bypass w/50ml @13:02:14.60 SamplePump.setPosition! 0ml @13:02:14.70 Bypass.open @13:02:16.28 Intake.open @13:02:17.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:02:19.70 Vacuum tare is 56% of full scale @13:02:19.95 Pressure.in :default @13:02:20.06 Pressure.hold @13:02:20.33 Gate.power :sampler,:ON @13:02:20.37 Waiting for external sampler to finish priming... @13:02:20.41 delayUntil "13:05:50.36" @13:05:50.41 SamplePump.in :prime @13:05:50.54 SamplePump.seek 50ml,2:53 @13:06:26.15 -> SP.rate 0.822470663019764 @13:06:51.58 Pressure.stop @13:06:51.68 SamplePump.setPosition! 0ml @13:06:51.76 Exhaust.open @13:06:53.24 Bypass.close @13:06:54.84 Pressure.in :default @13:06:54.96 Pressure.hold @13:06:55.32 SamplePump.in :prime @13:06:55.43 SamplePump.seek 150ml,7:39 @13:06:58.56 SamplePump.stop @13:06:58.72 Intake.close @13:06:58.81 Exhaust.close @13:06:59.71 Pressure.stop @13:06:59.85 Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made only 6.2psi vacuum after 2.3ml @13:08:45.18 -> #Hi Chris, you can message me here also if you are on @13:08:58.49 -> BACsampler.prime @13:08:58.62 Priming sample loop w/150ml, bypass w/50ml @13:08:58.64 SamplePump.setPosition! 0ml @13:08:58.75 Bypass.open @13:09:00.32 Intake.open @13:09:01.12 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:09:03.72 Vacuum tare is 56% of full scale @13:09:03.96 Pressure.in :default @13:09:04.07 Pressure.hold @13:09:04.35 SamplePump.in :prime @13:09:04.47 SamplePump.seek 50ml,2:53 @13:09:21.53 -> SP.rate 0.822470663019764 @13:09:44.35 -> #Hi Ben. Nice to meet you. @13:09:57.62 -> #Is the external pump still running. @13:10:05.49 Pressure.stop @13:10:05.59 SamplePump.setPosition! 0ml @13:10:05.68 Exhaust.open @13:10:07.15 Bypass.close @13:10:09.23 Pressure.in :default @13:10:09.34 Pressure.hold @13:10:09.68 SamplePump.in :prime @13:10:09.82 SamplePump.seek 150ml,7:39 @13:10:20.55 -> SP.rate 0.822470663019764 @13:10:24.35 -> SP.rate 0.822470663019764 @13:10:29.06 -> SP.rate 0.822470663019764 @13:11:06.85 -> #Ben I'm sure you caught on... SP.rate gives the ml per second @13:11:20.25 -> #useful if the ESP isn't reporting it. @13:12:53.80 Session Begun @13:13:01.07 -> SP.rate 0.822470663019764 @13:13:12.17 Pressure.stop BACsampler @13:13:12.41 -> # I di see that thnak you! @13:13:30.78 -> #looks good to me. I think you are good to go @13:14:12.73 -> # great thank you, we are going to move to the site and will report back when sampling @13:14:31.25 -> exit @13:14:31.27 Session Ended @13:14:35.12 -> #One thing: close thebbulkheads @13:14:39.14 -> exit @13:14:39.17 Session Ended @13:14:48.69 -> Intake.close @13:14:48.71 Intake.close Intake is closed @13:14:52.03 -> Exhaust.close @13:14:52.06 Exhaust.close Exhaust is closed @13:15:01.43 -> exit @13:15:01.45 Session Ended @13:24:01.35 Slide::Error in core -- Storage Carousel (SC) positionErr at 42 (sensed 42 + 24 counts) @13:52:19.11 Session Begun @13:53:31.38 -> ESP.reready! @13:53:31.43 Archive.in :default @13:53:31.50 Air.in :default @13:53:31.60 Lysis1.in :default @13:53:31.71 Lysis2.in :default @13:53:31.91 Plunge.in :default @13:53:32.03 Archive.home.jog 0 @13:53:32.13 Toroid.in :default @13:53:32.19 Carousel.in :default @13:53:32.32 PinPort.in :default @13:53:32.39 Air.home.jog 0 @13:53:32.43 Lysis1.home.jog 0 @13:53:32.60 Lysis2.home.jog 0 @13:53:32.64 Plunge.home.jog 0 @13:53:33.05 PinPort.home.jog 0 @13:53:33.16 Carousel.home.jog 0 @13:53:33.23 Toroid.home.jog 0 @13:53:33.91 Archive.in :homing @13:53:34.31 Archive.jog 1388 @13:53:34.39 Toroid.in :homing @13:53:34.43 Lysis2.in :homing @13:53:34.47 Air.in :homing @13:53:34.54 PinPort.in :homing @13:53:34.59 Plunge.in :homing @13:53:34.63 Lysis1.in :homing @13:53:35.01 Lysis2.jog 1395 @13:53:35.07 Toroid.jog 599 @13:53:35.11 Plunge.jog 407 @13:53:35.17 PinPort.jog -108 @13:53:35.21 Air.jog 1366 @13:53:35.26 Lysis1.jog 1390 @13:53:35.75 PinPort.setRawPosition! 0 @13:53:35.87 PinPort.seek :home @13:53:35.98 Archive.setRawPosition! 50 @13:53:36.07 Archive.seek :home @13:53:36.19 PinPort.in :default @13:53:36.23 Plunge.setRawPosition! 50 @13:53:36.41 Plunge.seek :home @13:53:36.56 Lysis2.setRawPosition! 50 @13:53:36.58 Archive.in :default @13:53:36.75 Lysis2.seek :home @13:53:36.89 Lysis1.setRawPosition! 50 @13:53:36.92 Air.setRawPosition! 50 @13:53:36.96 Plunge.in :default @13:53:37.15 Lysis1.seek :home @13:53:37.19 Air.seek :home @13:53:37.44 Toroid.setRawPosition! 0 @13:53:37.46 Lysis2.in :default @13:53:37.60 Toroid.seek :home @13:53:38.33 Toroid.in :default @13:53:38.37 Lysis1.in :default @13:53:38.41 Air.in :default @13:53:38.89 Toroid.seek :clear @13:53:40.51 Carousel.in :default @13:53:40.68 Carousel.home.seek 42 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 150ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Pressure = Absolute Pressure (Leak) Sensor @14psia PinPort = Handoff Pin Port (PP) clear @13:53:46.34 -> Cmd.stop @13:53:46.59 Carousel.in :default @13:53:46.74 Carousel.home.seek 42 @13:53:47.11 Lysis1.in :fast @13:53:47.21 Lysis1.seek :locked @13:53:49.76 Plunge.seek :locked @13:53:51.18 Lysis1.in :unlocking @13:53:51.38 Lysis1.seek :clear @13:53:52.91 Gate.power :motor,:OFF @13:53:52.93 Gate.power :core,:OFF @13:53:52.97 Gate.power :sampler,:OFF @13:53:53.01 Gate.power :analytic1,:OFF @13:53:53.04 Gate.power :analytic2,:OFF @13:53:53.09 Gate.power :main,:OFF @13:53:53.11 Safely stopped and ready to power off :STOPPED @13:53:55.69 -> exit @13:53:55.72 Session Ended @14:17:09.24 Session Begun @14:17:13.77 -> clients [ESP["ben"]] @14:18:53.22 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @14:18:53.48 Gate.power :sampler,:OFF,limit to 3500mA @14:18:53.51 Gate.power :main,:ON,limit to 5000mA @14:18:53.56 Gate.power :analytic1,:ON,limit to 5000mA @14:18:53.59 Gate.power :analytic2,:ON,limit to 5000mA @14:18:53.69 Powering core bus @14:18:56.10 SPR Module 1, FW Rev. B, FPGA Rev. C @14:18:56.47 Archive.configure :default @14:18:56.63 Air.configure :default @14:18:56.72 Lysis1.configure :default @14:18:56.81 Lysis2.configure :default @14:18:56.92 Plunge.configure :default @14:18:57.06 SamplePump.configure :default @14:18:57.30 Toroid.configure :default @14:18:57.47 Carousel.configure :default @14:18:57.59 Pressure.configure :default @14:18:57.71 ExtraPump.configure :default @14:18:57.84 PinPort.configure :default @14:18:57.93 All elf controllers running firmware version 4.84 @14:18:57.97 Gate.power :motor,:ON,limit to 5000mA @14:18:58.40 Air.home.jog 0 @14:18:58.48 Archive.home.jog 0 @14:18:59.23 Plunge.home.jog 0 @14:18:59.37 Carousel.home.jog 0 @14:18:59.43 Toroid.home.jog 0 @14:18:59.50 Lysis1.home.jog 0 @14:18:59.64 Lysis2.home.jog 0 @14:18:59.72 PinPort.home.jog 0 @14:19:01.30 Archive.in :homing @14:19:01.43 Air.in :homing @14:19:01.58 Archive.jog 1388 @14:19:01.64 Air.jog 1368 @14:19:01.97 Toroid.in :homing @14:19:02.02 Plunge.in :homing @14:19:02.15 Lysis1.in :homing @14:19:02.34 PinPort.in :homing @14:19:02.44 Lysis2.in :homing @14:19:02.88 Toroid.jog 603 @14:19:02.93 Plunge.jog 1565 @14:19:03.35 PinPort.jog -107 @14:19:03.49 Lysis1.jog 1388 @14:19:03.52 Lysis2.jog 1396 @14:19:03.89 PinPort.setRawPosition! 0 @14:19:03.96 Air.setRawPosition! 50 @14:19:04.03 Archive.setRawPosition! 50 @14:19:04.37 Air.seek :home @14:19:04.47 PinPort.seek :home @14:19:04.51 Archive.seek :home @14:19:04.92 Plunge.setRawPosition! 50 @14:19:04.97 Lysis2.setRawPosition! 50 @14:19:05.01 PinPort.in :default @14:19:05.04 Lysis1.setRawPosition! 50 @14:19:05.31 Plunge.seek :home @14:19:05.42 Lysis2.seek :home @14:19:05.47 Lysis1.seek :home @14:19:05.85 Toroid.setRawPosition! 0 @14:19:05.89 Air.in :default @14:19:05.99 Archive.in :default @14:19:06.09 Toroid.seek :home @14:19:06.36 Plunge.in :default @14:19:06.40 Lysis2.in :default @14:19:06.45 Toroid.in :default @14:19:06.53 Lysis1.in :default @14:19:07.07 Toroid.seek :clear @14:19:08.71 Carousel.in :default @14:19:08.93 Carousel.home.seek 42 @14:19:09.27 Selecting Cartridge 41 @14:19:09.63 Carousel.seek 41 @14:19:10.21 Lysis1.in :fast @14:19:10.31 Lysis1.seek :locked @14:19:13.14 Plunge.seek :locked @14:19:13.36 Air.in :fast @14:19:13.45 Air.seek :locked @14:19:15.09 Carousel.reconfigure :locked @14:19:15.41 Powering cartridge bus @14:19:18.07 Gate.setIR 1750,5000,0,6 @14:19:18.20 Gate.setIR 0,5000,0,6 @14:19:18.30 Gate.power :cartridge,:OFF @14:19:18.86 Mounted archive Cartridge @14:19:19.24 Preparing to filter cartridge 41 @14:19:20.06 Gate.power :sampler,:ON @14:19:20.14 Bypass.open @14:19:20.98 Toroid.in :hold @14:19:21.08 Toroid.seek :crack @14:19:22.68 Toroid.seek :cartridge @14:19:23.88 Toroid.in :default @14:19:23.99 Toroid.seek :clear @14:19:25.92 Exhaust.open @14:19:26.98 Pressurizing cartridge to 26psi above 13.9psia @14:19:27.00 SamplePump.setPosition! 0ml @14:19:27.08 Intake.open @14:19:27.84 Bypass.close @14:19:28.60 Exhaust.close @14:19:29.49 Pressure.in :default @14:19:29.59 Pressure.hold @14:19:29.90 Waiting for external sampler to finish priming... @14:19:29.92 delayUntil "14:22:50.09" @14:22:10.35 Session Begun @14:22:19.07 -> showlog -20 @14:22:50.14 SamplePump.in :charge @14:22:50.27 SamplePump.seek 2.3ml,42 seconds @14:23:03.17 Pressure.stop @14:23:03.28 Intake.close @14:23:04.09 Pressurized cartridge with 0.748ml to 26.0psi @14:23:04.11 Isolated @34.0psia (20.0psi) @14:23:04.17 Verifying cartridge holds at least 0.65..0.85 of 34.0psia @14:23:04.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:23:08.85 Cartridge pressure fell 1% to 33.7psia @14:23:08.99 Sampling 1000.0ml @14:23:09.02 SamplePump.setPosition! 0ml @14:23:09.15 Exhaust.open @14:23:09.96 Intake.open @14:23:10.85 Pressure.in :default @14:23:10.95 Pressure.hold @14:23:11.15 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:23:11.39 SamplePump.in :sample @14:23:11.51 SamplePump.seek 1000ml,1:23:51 @14:23:14.52 Cartridge 41 filtering 1/1000ml at 0.61ml/s with 24.2psi vacuum @14:23:45.55 -> clients [ESP["ben"],ESP["ben#2"]] @14:24:14.51 Cartridge 41 filtering 33/1000ml at 0.53ml/s with 25.7psi vacuum @14:25:14.52 Cartridge 41 filtering 63/1000ml at 0.49ml/s with 20.6psi vacuum @14:26:14.51 Cartridge 41 filtering 92/1000ml at 0.43ml/s with 28.7psi vacuum @14:27:15.03 Cartridge 41 filtering 119/1000ml at 0.46ml/s with 27.0psi vacuum @14:28:14.52 Cartridge 41 filtering 145/1000ml at 0.45ml/s with 26.4psi vacuum @14:28:53.12 Session Ended @14:29:14.52 Cartridge 41 filtering 169/1000ml at 0.41ml/s with 27.3psi vacuum @14:30:14.52 Cartridge 41 filtering 192/1000ml at 0.35ml/s with 29.5psi vacuum @14:31:14.52 Cartridge 41 filtering 214/1000ml at 0.38ml/s with 27.3psi vacuum @14:32:14.52 Cartridge 41 filtering 235/1000ml at 0.35ml/s with 28.7psi vacuum @14:33:14.53 Cartridge 41 filtering 255/1000ml at 0.30ml/s with 22.5psi vacuum @14:34:14.51 Cartridge 41 filtering 273/1000ml at 0.31ml/s with 17.7psi vacuum @14:35:14.52 Cartridge 41 filtering 291/1000ml at 0.34ml/s with 21.1psi vacuum @14:36:14.52 Cartridge 41 filtering 308/1000ml at 0.24ml/s with 21.4psi vacuum @14:37:14.52 Cartridge 41 filtering 324/1000ml at 0.22ml/s with 25.7psi vacuum @14:38:14.52 Cartridge 41 filtering 339/1000ml at 0.23ml/s with 26.9psi vacuum @14:39:14.51 Cartridge 41 filtering 354/1000ml at 0.27ml/s with 20.5psi vacuum @14:40:14.51 Cartridge 41 filtering 367/1000ml at 0.25ml/s with 28.7psi vacuum @14:41:14.51 Cartridge 41 filtering 381/1000ml at 0.26ml/s with 21.5psi vacuum @14:41:43.59 Flow averaged 0.35ml/s over 18:33 @14:41:43.66 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @14:41:44.26 Relieved excess vacuum in .7 seconds @14:41:44.32 SamplePump.seek 1000ml,51:34 @14:42:14.51 Cartridge 41 filtering 394/1000ml at 0.22ml/s with 27.5psi vacuum @14:43:14.51 Cartridge 41 filtering 409/1000ml at 0.21ml/s with 29.0psi vacuum @14:43:49.09 Session Ended @14:44:14.51 Cartridge 41 filtering 424/1000ml at 0.20ml/s with 26.6psi vacuum @14:45:14.51 Cartridge 41 filtering 438/1000ml at 0.20ml/s with 25.3psi vacuum @14:46:14.51 Cartridge 41 filtering 452/1000ml at 0.20ml/s with 23.9psi vacuum @14:47:14.51 Cartridge 41 filtering 465/1000ml at 0.20ml/s with 29.5psi vacuum @14:47:44.53 Flow averaged 0.23ml/s over 6:01 @14:47:44.57 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @14:47:45.18 Relieved excess vacuum in .7 seconds @14:47:45.21 SamplePump.seek 1000ml,44:33 @14:48:14.51 Cartridge 41 filtering 478/1000ml at 0.22ml/s with 24.9psi vacuum @14:48:16.66 Flow averaged 0.22ml/s over 32 seconds @14:48:16.71 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi @14:48:17.31 Relieved excess vacuum in .7 seconds @14:48:17.34 SamplePump.seek 1000ml,43:59 @14:48:51.68 Flow averaged 0.22ml/s over 35 seconds @14:48:51.72 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @14:48:52.32 Relieved excess vacuum in .7 seconds @14:48:52.35 SamplePump.seek 1000ml,43:22 @14:49:06.93 Flow averaged 0.21ml/s over 15 seconds @14:49:06.98 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @14:49:08.02 Relieved excess vacuum in 1.1 seconds @14:49:08.06 SamplePump.seek 1000ml,43:07 @14:49:14.51 Cartridge 41 filtering 490/1000ml at 0.22ml/s with 21.7psi vacuum @14:49:22.53 Flow averaged 0.21ml/s over 15 seconds @14:49:22.58 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @14:49:23.18 Relieved excess vacuum in .7 seconds @14:49:23.22 SamplePump.seek 1000ml,42:52 @14:49:34.97 Flow averaged 0.21ml/s over 12 seconds @14:49:35.01 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @14:49:35.62 Relieved excess vacuum in .7 seconds @14:49:35.65 SamplePump.seek 1000ml,42:39 @14:49:43.09 Flow estimate reduced to 0.17ml/s @14:49:43.12 Intake.close @14:49:43.17 Exhaust.close @14:49:43.48 Toroid.in :hold @14:49:43.56 Toroid.seek :bypass @14:49:45.21 Toroid.in :default @14:49:45.28 Toroid.seek :clear @14:49:46.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:49:46.92 Pressure.stop @14:49:47.15 Sampled 495.8ml @14:49:47.86 Plunge.seek :reagent @14:49:48.68 Sample loop closed. Ready to Deliver Reagents @14:49:48.70 Archive.seek :sealed @14:49:50.28 Gate.power :sampler,:OFF @14:49:52.63 Archive.seek :evac @14:50:06.66 delay 1:00 @14:51:06.70 Archive.seek :clear @14:51:24.18 RNAlater soak for 10:00 @14:58:34.99 Session Begun @14:58:46.90 -> showlog -20 @14:59:06.43 -> Can Can@14:57:45, 27.0C, 38% humidity, 14.3psia, 14.866V, 0.358A, 1.042Ah, 5.32W @15:01:24.22 Archive.seek :tVent @15:01:26.71 delay 1 second @15:01:27.74 Archive.seek :evac @15:01:43.32 delay 10 seconds @15:01:53.36 Archive.seek :tVent @15:02:08.92 delay 1 second @15:02:09.95 Archive.seek :evac @15:02:25.50 delay 10 seconds @15:02:35.54 Sample preserved and evacuated @15:02:35.70 Selecting Cartridge 40 @15:02:36.01 Carousel.in :default @15:02:36.17 Carousel.home.seek 41 @15:02:36.56 Archive.in :unlocking @15:02:36.73 Archive.seek :clear @15:02:36.86 Air.in :unlocking @15:02:37.03 Air.seek :clear @15:02:41.27 Lysis1.in :unlocking @15:02:41.47 Lysis1.seek :clear @15:02:41.57 Plunge.in :unlocking @15:02:41.70 Plunge.seek :clear @15:02:43.13 Carousel.seek 40 @15:02:43.71 Lysis1.in :fast @15:02:43.84 Lysis1.seek :locked @15:02:46.50 Plunge.in :default @15:02:46.60 Plunge.seek :locked @15:02:46.80 Air.in :fast @15:02:46.90 Air.seek :locked @15:02:48.49 Carousel.reconfigure :locked @15:02:48.69 Powering cartridge bus @15:02:51.38 Gate.setIR 1750,5000,0,6 @15:02:51.52 Gate.setIR 0,5000,0,6 @15:02:51.61 PuckHeater.configure :default @15:02:51.70 Gate.power :heat,:ON,limit to 5000mA @15:02:54.45 Mounted lysis Cartridge board#Q05058 @15:02:54.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:02:54.88 Preparing to filter cartridge 40 @15:02:55.14 Gate.power :sampler,:ON @15:02:55.28 Priming sample loop w/150ml, bypass w/50ml @15:02:55.30 SamplePump.setPosition! 0ml @15:02:55.38 Bypass.open @15:02:56.91 Intake.open @15:02:57.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:03:00.28 Vacuum tare is 56% of full scale @15:03:00.47 Pressure.in :default @15:03:00.56 Pressure.hold @15:03:00.82 Waiting for external sampler to finish priming... @15:03:00.84 delayUntil "15:06:25.16" @15:06:25.22 SamplePump.in :prime @15:06:25.32 SamplePump.seek 50ml,2:53 @15:07:26.30 Pressure.stop @15:07:26.39 SamplePump.setPosition! 0ml @15:07:26.46 Exhaust.open @15:07:27.99 Bypass.close @15:07:29.61 Pressure.in :default @15:07:29.70 Pressure.hold @15:07:30.06 SamplePump.in :prime @15:07:30.17 SamplePump.seek 150ml,7:39 @15:10:32.46 Pressure.stop @15:10:32.57 Bypass.open @15:10:33.31 Intake.close @15:10:34.11 Toroid.in :hold @15:10:34.21 Toroid.seek :crack @15:10:35.81 Toroid.seek :cartridge @15:10:36.96 Toroid.in :default @15:10:37.06 Toroid.seek :clear @15:10:39.11 Pressurizing cartridge to 26psi above 13.3psia @15:10:39.13 SamplePump.setPosition! 0ml @15:10:39.23 Intake.open @15:10:39.87 Bypass.close @15:10:40.59 Exhaust.close @15:10:41.45 Pressure.in :default @15:10:41.54 Pressure.hold @15:10:41.85 SamplePump.in :charge @15:10:41.95 SamplePump.seek 2.3ml,42 seconds @15:10:56.13 Pressure.stop @15:10:56.23 Intake.close @15:10:57.00 Pressurized cartridge with 0.908ml to 26.0psi @15:10:57.02 Isolated @33.2psia (20.0psi) @15:10:57.06 Verifying cartridge holds at least 0.65..0.85 of 33.2psia @15:10:57.11 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:11:01.84 Sampling 50.0ml @15:11:01.86 SamplePump.setPosition! 0ml @15:11:01.95 Exhaust.open @15:11:02.69 Intake.open @15:11:03.52 Pressure.in :default @15:11:03.61 Pressure.hold @15:11:03.80 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @15:11:04.58 SamplePump.in :sample @15:11:04.69 SamplePump.seek 50ml,4:41 @15:11:07.71 Cartridge 40 filtering 1/50ml at 0.62ml/s with 22.9psi vacuum @15:12:07.72 Cartridge 40 filtering 37/50ml at 0.57ml/s with 26.8psi vacuum @15:12:32.76 Pressure.stop @15:12:32.94 Intake.close @15:12:32.98 Exhaust.close @15:12:33.28 Toroid.in :hold @15:12:33.38 Toroid.seek :bypass @15:12:35.05 Toroid.in :default @15:12:35.15 Toroid.seek :clear @15:12:36.80 Sampled 50.0ml @15:12:38.53 SPR.command "read_sequences" @15:12:39.29 SPR.command "stats_on" @15:12:39.47 SPR.command "start 0 1" @15:12:39.96 Gate.power :sampler,:OFF @15:12:40.18 SPR.start /var/log/esp/spr23aug0915h.spr.bz2 @15:12:42.15 Handoff.select :waste @15:12:48.89 Syringe tare is 18% of full scale @15:12:48.93 PinPort.in :locking @15:12:49.04 PinPort.seek :locked @15:12:51.62 Archive.in :fast @15:12:51.70 Lysis2.in :fast @15:12:51.76 Lysis1.seek 1350uL @15:12:52.02 Archive.seek :full @15:12:52.09 Lysis2.seek 950uL @15:12:56.88 Plunge.seek :reagent @15:12:58.42 Evacuating puck with filter bubble point of 50psi @15:12:58.46 PinPort.seek :locked @15:12:58.60 Handoff.select :waste @15:13:05.23 Air syringe pumping up to 1 strokes to reach 8psi @15:13:05.24 Air stroke #1 of 1 @15:13:05.42 Air.in :pressurize @15:13:05.52 Air.seek :pressurized @15:13:11.30 1.6psi after pressurize @15:13:11.32 Air.in :compress @15:13:11.43 Air.seek :evac @15:13:14.48 0.9psi after compress @15:13:32.09 0.4psi after compress (stable) @15:13:32.11 Air.in :default @15:13:32.22 Air.hold @15:13:32.47 at 0.3psi Opened to waste @15:13:32.49 Handoff.select :blocked @15:13:32.67 Archive syringe pumping up to 8 strokes to reach 50psi @15:13:32.69 Archive stroke #1 of 8 @15:13:32.85 Archive.in :pressurize @15:13:32.95 Archive.seek :pressurized @15:13:45.48 1.2psi after pressurize @15:13:54.58 1.2psi after pressurize (stable) @15:13:54.61 Archive.in :compress @15:13:54.73 Archive.seek :empty @15:14:09.39 7.4psi after compress @15:14:26.99 15.3psi after compress (stable) @15:14:27.02 Archive.in :gulp @15:14:27.13 Archive.seek :topVent @15:14:33.42 15.6psi after gulp @15:14:39.55 15.6psi after gulp (stable) @15:14:39.56 Archive stroke #2 of 8 @15:14:39.71 Archive.in :pressurize @15:14:39.84 Archive.seek :pressurized @15:14:52.42 15.6psi after pressurize @15:14:58.02 15.5psi after pressurize (stable) @15:14:58.05 Archive.in :compress @15:14:58.15 Archive.seek :empty @15:15:12.81 24.4psi after compress @15:15:24.43 26.0psi after compress (stable) @15:15:24.48 Archive.in :gulp @15:15:24.59 Archive.seek :topVent @15:15:30.88 26.1psi after gulp @15:15:36.48 26.1psi after gulp (stable) @15:15:36.50 Archive stroke #3 of 8 @15:15:36.67 Archive.in :pressurize @15:15:36.78 Archive.seek :pressurized @15:15:49.33 26.0psi after pressurize @15:15:54.93 26.0psi after pressurize (stable) @15:15:54.96 Archive.in :compress @15:15:55.06 Archive.seek :empty @15:16:09.71 36.3psi after compress @15:16:20.32 37.9psi after compress (stable) @15:16:20.35 Archive.in :gulp @15:16:20.45 Archive.seek :topVent @15:16:26.74 37.9psi after gulp @15:16:32.34 37.9psi after gulp (stable) @15:16:32.35 Archive stroke #4 of 8 @15:16:32.51 Archive.in :pressurize @15:16:32.60 Archive.seek :pressurized @15:16:45.12 37.7psi after pressurize @15:16:50.72 37.7psi after pressurize (stable) @15:16:50.76 Archive.in :compress @15:16:50.87 Archive.seek :empty @15:17:05.53 47.6psi after compress @15:17:16.65 49.3psi after compress (stable) @15:17:16.68 Archive.in :gulp @15:17:16.85 Archive.seek :topVent @15:17:18.92 Reached 49.4 of 50.0psi after pumping 6920uL (4.00 strokes) @15:17:18.94 Archive.in :default @15:17:19.06 Archive.hold @15:17:19.16 Handoff.select :waste @15:17:34.93 delay 1:00 @15:18:34.97 Handoff.select :blocked @15:18:57.70 Handoff.select :waste @15:18:57.88 0.3psi at evacEnd @15:18:57.90 Handoff.select :blocked @15:18:58.07 Archive.in :fast @15:18:58.20 Archive.seek :topVent @15:19:10.30 Stable @0.3psi after Handoff blocked @15:19:10.33 Archive syringe pumping up to 8 strokes to reach 30psi @15:19:10.37 Archive stroke #1 of 8 @15:19:10.54 Archive.in :pressurize @15:19:10.64 Archive.seek :pressurized @15:19:23.17 0.4psi after pressurize @15:19:23.20 Archive.in :compress @15:19:23.32 Archive.seek :empty @15:19:37.97 9.3psi after compress @15:19:48.57 10.5psi after compress (stable) @15:19:48.60 Archive.in :gulp @15:19:48.71 Archive.seek :topVent @15:19:55.49 10.6psi after gulp @15:20:01.09 10.6psi after gulp (stable) @15:20:01.11 Archive stroke #2 of 8 @15:20:01.27 Archive.in :pressurize @15:20:01.36 Archive.seek :pressurized @15:20:13.94 10.6psi after pressurize @15:20:13.97 Archive.in :compress @15:20:14.09 Archive.seek :empty @15:20:28.75 18.7psi after compress @15:20:39.88 20.3psi after compress (stable) @15:20:39.91 Archive.in :gulp @15:20:40.02 Archive.seek :topVent @15:20:46.31 20.3psi after gulp @15:20:51.91 20.3psi after gulp (stable) @15:20:51.92 Archive stroke #3 of 8 @15:20:52.07 Archive.in :pressurize @15:20:52.16 Archive.seek :pressurized @15:21:04.68 20.3psi after pressurize @15:21:04.72 Archive.in :compress @15:21:04.84 Archive.seek :empty @15:21:19.52 28.3psi after compress @15:21:30.62 30.0psi after compress (stable) @15:21:30.65 Archive.in :gulp @15:21:30.82 Archive.seek :topVent @15:21:37.14 30.0psi after gulp @15:21:42.80 30.0psi after gulp (stable) @15:21:42.81 Archive stroke #4 of 8 @15:21:42.97 Archive.in :pressurize @15:21:43.06 Archive.seek :pressurized @15:21:55.59 29.9psi after pressurize @15:21:55.72 Archive.in :compress @15:21:55.85 Archive.seek :empty @15:22:02.27 Reached 30.4 of 30.3psi after pumping 6741uL (3.90 strokes) @15:22:02.30 Archive.in :default @15:22:02.41 Archive.hold @15:22:02.52 Handoff.select :waste @15:22:22.31 delay 1:00 @15:23:22.33 Handoff.select :blocked @15:23:45.07 Handoff.select :waste @15:23:45.22 0.5psi at evacEnd @15:23:45.25 Air.seek :fullVent @15:23:46.71 Archive.in :fast @15:23:46.81 Archive.seek :topVent @15:23:53.31 Seawater evacuated, Pressure above/below filter released. @15:23:53.44 Lysis1.in :slow @15:23:53.53 Lysis1.seek 1050uL @15:24:23.56 delay 5 seconds @15:24:28.58 Handoff.select :blocked @15:24:28.82 Lysis1.seek 450uL @15:25:28.79 delay 3 seconds @15:25:31.90 Air.in mix with 1.40048 parts Lysis1 @15:25:32.55 Air.seek :evac @15:25:32.62 Lysis1.seek 300uL @15:25:47.79 Lysis1.seek :empty @15:26:17.94 Lysis buffer delivered @3.8psi @15:26:17.98 Polling Can every 2:00 @15:26:18.55 PuckHeater.configure :default #ambient 27.4C @15:26:18.63 PuckHeater.seek 79.5C @15:27:07.72 PuckHeater.hold 2:00,79.5C @15:29:07.84 Polling Can every 2:00 @15:29:08.39 PuckHeater.configure :default #ambient 27.4C @15:29:08.48 PuckHeater.hold 30 seconds,78.5C @15:29:38.70 Polling Can every 2:00 @15:29:39.26 PuckHeater.configure :default #ambient 27.4C @15:29:39.34 PuckHeater.hold 30 seconds,78C @15:30:09.60 Polling Can every 2:00 @15:30:10.17 PuckHeater.hold 10:00,77.5C @15:31:26.84 -> Can Can@15:30:10, 27.4C, 38% humidity, 14.3psia, 14.858V, 0.469A, 1.505Ah, 6.96W @15:40:10.01 Lysis complete. Syringe pressure @6.7psi @15:40:10.06 Archive syringe pumping up to 5 strokes to reach 40psi @15:40:10.07 Archive stroke #1 of 5 @15:40:10.21 Archive.in :pressurize @15:40:10.36 Archive.seek :pressurized @15:40:22.89 6.7psi after pressurize @15:40:28.50 6.6psi after pressurize (stable) @15:40:28.53 Archive.in :compress @15:40:28.63 Archive.seek :empty @15:40:43.29 19.2psi after compress @15:40:53.90 20.6psi after compress (stable) @15:40:53.93 Archive.in :gulp @15:40:54.10 Archive.seek :topVent @15:41:00.50 20.7psi after gulp @15:41:06.14 20.7psi after gulp (stable) @15:41:06.17 Archive stroke #2 of 5 @15:41:06.32 Archive.in :pressurize @15:41:06.42 Archive.seek :pressurized @15:41:18.94 20.5psi after pressurize @15:41:24.54 20.4psi after pressurize (stable) @15:41:25.01 Archive.in :compress @15:41:25.23 Archive.seek :empty @15:41:39.90 31.7psi after compress @15:41:50.51 33.1psi after compress (stable) @15:41:50.56 Archive.in :gulp @15:41:50.69 Archive.seek :topVent @15:41:56.98 33.2psi after gulp @15:42:02.58 33.2psi after gulp (stable) @15:42:02.60 Archive stroke #3 of 5 @15:42:02.74 Archive.in :pressurize @15:42:02.86 Archive.seek :pressurized @15:42:15.39 33.0psi after pressurize @15:42:20.99 32.9psi after pressurize (stable) @15:42:21.02 Archive.in :compress @15:42:21.12 Archive.seek :empty @15:42:32.96 Reached 40.2 of 40.0psi after pumping 5128uL (2.96 strokes) @15:42:32.98 Archive.in :default @15:42:33.09 Archive.hold @15:42:33.23 delay 2 seconds @15:42:35.26 Archive.in :fast @15:42:35.35 Archive.seek :full @15:42:42.35 Lysis1.seek 138uL @15:42:56.25 delay 1 second @15:42:57.29 Archive.seek :full @15:42:57.33 Lysis1.seek :empty @15:43:11.29 Handoff.select :blocked @15:43:11.44 Initial air pressure @42.4psi @15:43:11.52 Lysis1.in :extract @15:43:11.68 Lysis1.seek 600uL,nil @15:43:17.39 Initial 600uL lysate @24.6psi @15:43:17.41 Handoff.select :blocked @15:43:17.58 Initial air pressure @25.0psi @15:43:17.70 Lysis1.seek 825uL,nil @15:43:20.20 Recovered 825uL lysate @21.3psi @15:43:20.23 Air.in :halfSpeed @15:43:20.33 Air.seek :fullVent @15:43:22.61 Puck Top Vented, bottom @23.7psi @15:43:22.63 Handoff.select :waste @15:43:22.80 delay 5 seconds @15:43:27.89 Handoff open to waste, bottom @0.5psi @15:43:27.92 Air.in :default @15:43:28.07 Air.seek :evac @15:43:29.51 Lysis2.in :DAmix @15:43:29.68 Lysis1.in :DAmix @15:43:30.44 Lysis2.seek 700uL @15:43:30.54 Lysis1.seek 575uL @15:44:05.59 delay 5 seconds @15:44:10.61 Handoff.select SPR @15:44:10.77 SPR.command "start 2 14" @15:44:11.92 SPR stabilized @15:44:12.12 delay 10 seconds @15:44:22.14 SPR.command "start 2 0" @15:44:25.83 Waiting up to 11:00 for SPR to enter state 1 @15:46:23.89 SPR.command "hold_on" @15:46:25.29 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:46:26.00 Lysis2.seek 400uL @15:46:26.06 Lysis1.seek 275uL @15:47:08.11 delay 15 seconds @15:47:23.13 SPR.command "hold_off" @15:47:25.94 Waiting up to 38:00 for SPR to enter state 7 @15:48:34.63 -> Can Can@15:47:35, 27.3C, 39% humidity, 14.3psia, 14.850V, 0.552A, 1.758Ah, 8.20W @15:51:31.07 -> exit @15:51:31.10 Session Ended @16:01:46.11 SPRsummary:90.3RIU,4e+09RIU,500RIU,482RIU,none,none,none after 3112s @16:13:16.56 SPR analysis completed @16:13:26.14 Ended log spr23aug0915h.spr.bz2 @16:13:26.16 SPR.command "stats_off" @16:13:26.63 Handoff.select :close @16:13:26.77 Gate.power :heat,:OFF @16:13:26.79 Gate.power :cartridge,:OFF @16:13:26.88 PinPort.in :unlocking @16:13:26.94 PinPort.seek :clear @16:13:28.86 Carousel.in :default @16:13:28.99 Carousel.home.seek 40 @16:13:29.33 Plunge.seek :locked @16:13:30.24 Archive.in :unlocking @16:13:30.42 Archive.seek :clear @16:13:30.47 Air.in :unlocking @16:13:30.69 Air.seek :clear @16:13:30.80 Lysis1.in :unlocking @16:13:30.97 Lysis1.seek :clear @16:13:31.04 Lysis2.in :unlocking @16:13:31.15 Lysis2.seek :clear @16:13:35.05 Gate.power :motor,:OFF @16:13:35.07 Gate.power :core,:OFF @16:13:35.09 Gate.power :analytic1,:OFF @16:13:35.10 Gate.power :analytic2,:OFF @16:13:35.11 Gate.power :main,:OFF @16:13:35.12 Safely stopped and ready to power off @05:06:37.10PDT10-Aug-23 Session Begun @05:06:41.19 -> Can Can@05:05:26, 23.7C, 43% humidity, 14.2psia, 14.884V, 0.081A, 3.032Ah, 1.21W @05:07:21.73 -> exit @05:07:21.75 Session Ended @07:43:52.77 Session Begun @07:43:58.40 -> Can Can@07:43:01, 23.2C, 43% humidity, 14.3psia, 14.885V, 0.081A, 3.244Ah, 1.21W @07:48:47.97 Session Begun @07:49:14.73 -> Can Can@07:49:00, 23.2C, 43% humidity, 14.3psia, 14.887V, 0.081A, 3.252Ah, 1.21W @07:53:46.97 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0915h.spr.bz2}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @07:54:23.60 -> slots 41:40 @07:54:23.66 SyntaxError in ben -- compile error ben:3: syntax error, unexpected ':', expecting $end slots 41:40 ^ @07:54:34.16 -> slots 40:41 @07:54:34.19 SyntaxError in ben -- compile error ben:4: syntax error, unexpected ':', expecting $end slots 40:41 ^ @07:54:45.70 -> slots 40 {40 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0915h.spr.bz2}} @07:55:00.39 -> slots 39 {39 => { Type: :archiveHiBiomass_bac, State: :dry}} @07:55:39.33 Session Begun @07:56:24.16 -> # hi every one @08:01:44.56 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @08:01:44.99 Gate.power :sampler,:OFF,limit to 3500mA @08:01:45.05 Gate.power :main,:ON,limit to 5000mA @08:01:45.10 Gate.power :analytic1,:ON,limit to 5000mA @08:01:45.19 Gate.power :analytic2,:ON,limit to 5000mA @08:01:45.32 Powering core bus @08:01:47.64 SPR Module 1, FW Rev. B, FPGA Rev. C @08:01:48.62 Archive.configure :default @08:01:48.78 Air.configure :default @08:01:48.89 Lysis1.configure :default @08:01:49.02 Lysis2.configure :default @08:01:49.16 Plunge.configure :default @08:01:49.33 SamplePump.configure :default @08:01:49.48 Toroid.configure :default @08:01:49.69 Carousel.configure :default @08:01:49.87 Pressure.configure :default @08:01:50.02 ExtraPump.configure :default @08:01:50.14 PinPort.configure :default @08:01:50.27 All elf controllers running firmware version 4.84 @08:01:50.30 Gate.power :motor,:ON,limit to 5000mA @08:01:50.81 Archive.home.jog 0 @08:01:51.44 Carousel.home.jog 0 @08:01:51.50 Toroid.home.jog 0 @08:01:51.59 Plunge.home.jog 0 @08:01:51.66 Lysis1.home.jog 0 @08:01:51.78 PinPort.home.jog 0 @08:01:51.88 Air.home.jog 0 @08:01:52.04 Lysis2.home.jog 0 @08:01:52.97 Archive.in :homing @08:01:53.28 Archive.jog 1393 @08:01:53.37 PinPort.in :homing @08:01:53.43 Plunge.in :homing @08:01:53.48 Lysis1.in :homing @08:01:53.58 Toroid.in :homing @08:01:53.69 Air.in :homing @08:01:53.77 Lysis2.in :homing @08:01:54.22 Plunge.jog 1571 @08:01:54.27 PinPort.jog -107 @08:01:54.39 Lysis1.jog 1388 @08:01:54.46 Toroid.jog 597 @08:01:54.57 Lysis2.jog 1394 @08:01:54.69 Air.jog 1371 @08:01:55.17 Archive.setRawPosition! 50 @08:01:55.20 PinPort.setRawPosition! 0 @08:01:55.39 Archive.seek :home @08:01:55.43 PinPort.seek :home @08:01:55.73 PinPort.in :default @08:01:55.94 Plunge.setRawPosition! 50 @08:01:56.04 Archive.in :default @08:01:56.20 Plunge.seek :home @08:01:56.40 Lysis1.setRawPosition! 50 @08:01:56.42 Air.setRawPosition! 50 @08:01:56.51 Lysis2.setRawPosition! 50 @08:01:56.75 Lysis1.seek :home @08:01:56.80 Lysis2.seek :home @08:01:56.86 Air.seek :home @08:01:57.24 Toroid.setRawPosition! 0 @08:01:57.25 Plunge.in :default @08:01:57.47 Toroid.seek :home @08:01:57.70 Lysis2.in :default @08:01:57.74 Lysis1.in :default @08:01:57.85 Toroid.in :default @08:01:57.95 Air.in :default @08:01:59.02 Toroid.seek :clear @08:02:00.70 Carousel.in :default @08:02:00.91 Carousel.home.seek 40 @08:02:01.25 Selecting Cartridge 39 @08:02:01.63 Carousel.seek 39 @08:02:02.24 Lysis1.in :fast @08:02:02.37 Lysis1.seek :locked @08:02:05.04 Plunge.seek :locked @08:02:05.27 Air.in :fast @08:02:05.40 Air.seek :locked @08:02:07.03 Carousel.reconfigure :locked @08:02:07.27 Powering cartridge bus @08:02:09.93 Gate.setIR 1750,5000,0,6 @08:02:10.06 Gate.setIR 0,5000,0,6 @08:02:10.19 Gate.power :cartridge,:OFF @08:02:10.80 Mounted archive Cartridge @08:02:11.22 Preparing to filter cartridge 39 @08:02:11.52 Gate.power :sampler,:ON @08:02:11.69 Priming sample loop w/150ml, bypass w/50ml @08:02:11.73 SamplePump.setPosition! 0ml @08:02:11.91 Bypass.open @08:02:13.47 Intake.open @08:02:14.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:02:16.88 Vacuum tare is 56% of full scale @08:02:17.12 Pressure.in :default @08:02:17.26 Pressure.hold @08:02:17.55 Waiting for external sampler to finish priming... @08:02:17.58 delayUntil "08:05:41.55" @08:04:39.50 -> slots 42 {42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}} @08:05:41.59 SamplePump.in :prime @08:05:41.74 SamplePump.seek 50ml,2:53 @08:06:42.81 Pressure.stop @08:06:42.93 SamplePump.setPosition! 0ml @08:06:43.04 Exhaust.open @08:06:44.55 Bypass.close @08:06:46.74 Pressure.in :default @08:06:46.86 Pressure.hold @08:06:47.25 SamplePump.in :prime @08:06:47.39 SamplePump.seek 150ml,7:39 @08:09:49.84 Pressure.stop @08:09:49.98 Bypass.open @08:09:50.74 Intake.close @08:09:51.57 Toroid.in :hold @08:09:51.70 Toroid.seek :crack @08:09:53.58 Toroid.seek :cartridge @08:09:54.76 Toroid.in :default @08:09:54.89 Toroid.seek :clear @08:09:56.96 Pressurizing cartridge to 26psi above 13.6psia @08:09:56.99 SamplePump.setPosition! 0ml @08:09:57.15 Intake.open @08:09:57.86 Bypass.close @08:09:58.63 Exhaust.close @08:09:59.55 Pressure.in :default @08:09:59.68 Pressure.hold @08:10:00.03 SamplePump.in :charge @08:10:00.18 SamplePump.seek 2.3ml,42 seconds @08:10:07.67 Pressure.stop @08:10:07.84 Intake.close @08:10:08.64 Pressurized cartridge with 0.246ml to 26.2psi @08:10:08.66 Isolated @33.8psia (20.2psi) @08:10:08.73 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @08:10:08.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:10:13.48 Cartridge pressure fell 8% to 31.0psia @08:10:13.70 Sampling 1000.0ml @08:10:13.73 SamplePump.setPosition! 0ml @08:10:13.98 Exhaust.open @08:10:14.78 Intake.open @08:10:15.65 Pressure.in :default @08:10:15.79 Pressure.hold @08:10:16.02 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:10:16.81 SamplePump.in :sample @08:10:16.96 SamplePump.seek 1000ml,1:23:51 @08:10:19.95 Cartridge 39 filtering 1/1000ml at 0.59ml/s with 31.0psi vacuum @08:11:19.97 Cartridge 39 filtering 36/1000ml at 0.53ml/s with 17.3psi vacuum @08:12:19.96 Cartridge 39 filtering 68/1000ml at 0.48ml/s with 16.5psi vacuum @08:13:19.95 Cartridge 39 filtering 99/1000ml at 0.51ml/s with 31.7psi vacuum @08:13:46.17 -> exit @08:13:46.20 Session Ended @08:14:19.95 Cartridge 39 filtering 129/1000ml at 0.53ml/s with 28.3psi vacuum @08:15:19.96 Cartridge 39 filtering 158/1000ml at 0.51ml/s with 29.1psi vacuum @08:16:19.96 Cartridge 39 filtering 187/1000ml at 0.43ml/s with 18.1psi vacuum @08:17:19.96 Cartridge 39 filtering 215/1000ml at 0.42ml/s with 28.7psi vacuum @08:18:19.96 Cartridge 39 filtering 242/1000ml at 0.42ml/s with 15.9psi vacuum @08:19:19.96 Cartridge 39 filtering 269/1000ml at 0.45ml/s with 30.5psi vacuum @08:20:19.96 Cartridge 39 filtering 294/1000ml at 0.45ml/s with 30.3psi vacuum @08:21:19.96 Cartridge 39 filtering 320/1000ml at 0.37ml/s with 14.1psi vacuum @08:21:48.11 -> Can Can@08:20:55, 23.9C, 43% humidity, 14.4psia, 14.823V, 0.843A, 3.521Ah, 12.49W @08:22:19.95 Cartridge 39 filtering 344/1000ml at 0.43ml/s with 18.3psi vacuum @08:23:19.96 Cartridge 39 filtering 368/1000ml at 0.42ml/s with 21.5psi vacuum @08:24:19.96 Cartridge 39 filtering 391/1000ml at 0.38ml/s with 30.2psi vacuum @08:25:19.96 Cartridge 39 filtering 413/1000ml at 0.31ml/s with 24.9psi vacuum @08:26:19.96 Cartridge 39 filtering 434/1000ml at 0.32ml/s with 19.9psi vacuum @08:27:19.96 Cartridge 39 filtering 454/1000ml at 0.37ml/s with 18.1psi vacuum 1.7m deep (17.1psia) @08:28:19.96 Cartridge 39 filtering 474/1000ml at 0.32ml/s with 19.2psi vacuum @08:29:19.96 Cartridge 39 filtering 493/1000ml at 0.31ml/s with 18.1psi vacuum @08:30:19.96 Cartridge 39 filtering 511/1000ml at 0.27ml/s with 27.7psi vacuum @08:31:19.96 Cartridge 39 filtering 528/1000ml at 0.32ml/s with 30.6psi vacuum @08:32:19.96 Cartridge 39 filtering 545/1000ml at 0.29ml/s with 21.3psi vacuum @08:33:19.96 Cartridge 39 filtering 562/1000ml at 0.29ml/s with 24.3psi vacuum @08:34:19.96 Cartridge 39 filtering 577/1000ml at 0.26ml/s with 14.1psi vacuum @08:35:19.96 Cartridge 39 filtering 592/1000ml at 0.22ml/s with 18.7psi vacuum @08:36:19.96 Cartridge 39 filtering 606/1000ml at 0.25ml/s with 15.3psi vacuum @08:37:19.96 Cartridge 39 filtering 620/1000ml at 0.26ml/s with 31.6psi vacuum @08:38:19.96 Cartridge 39 filtering 633/1000ml at 0.22ml/s with 17.8psi vacuum @08:38:55.22 Flow averaged 0.37ml/s over 28:39 @08:38:55.36 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @08:38:56.39 Relieved excess vacuum in 1.2 seconds @08:38:56.45 SamplePump.seek 1000ml,30:24 @08:39:19.95 Cartridge 39 filtering 647/1000ml at 0.27ml/s with 17.3psi vacuum @08:39:59.53 Flow averaged 0.22ml/s over 1:04 @08:39:59.63 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi @08:40:00.23 Relieved excess vacuum in .7 seconds @08:40:00.30 SamplePump.seek 1000ml,29:13 @08:40:19.95 Cartridge 39 filtering 660/1000ml at 0.20ml/s with 27.6psi vacuum @08:40:27.84 Flow averaged 0.22ml/s over 28 seconds @08:40:27.94 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @08:40:28.55 Relieved excess vacuum in .7 seconds @08:40:28.61 SamplePump.seek 1000ml,28:43 @08:40:58.29 Flow averaged 0.22ml/s over 30 seconds @08:40:58.43 Waiting up to 14 seconds for vacuum to reduce from 28.3 to 18.0psi @08:40:59.04 Relieved excess vacuum in .8 seconds @08:40:59.11 SamplePump.seek 1000ml,28:10 @08:41:19.96 Cartridge 39 filtering 673/1000ml at 0.26ml/s with 23.1psi vacuum @08:41:21.57 Flow averaged 0.22ml/s over 23 seconds @08:41:21.66 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @08:41:22.27 Relieved excess vacuum in .7 seconds @08:41:22.34 SamplePump.seek 1000ml,27:45 @08:41:36.53 Flow averaged 0.22ml/s over 15 seconds @08:41:36.62 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @08:41:37.23 Relieved excess vacuum in .7 seconds @08:41:37.27 SamplePump.seek 1000ml,27:30 @08:41:47.42 Flow averaged 0.21ml/s over 11 seconds @08:41:47.51 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @08:41:48.13 Relieved excess vacuum in .7 seconds @08:41:48.20 SamplePump.seek 1000ml,27:19 @08:42:00.59 Flow averaged 0.21ml/s over 13 seconds @08:42:00.69 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @08:42:01.30 Relieved excess vacuum in .7 seconds @08:42:01.36 SamplePump.seek 1000ml,27:06 @08:42:12.84 Flow averaged 0.22ml/s over 12 seconds @08:42:12.94 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @08:42:13.54 Relieved excess vacuum in .7 seconds @08:42:13.61 SamplePump.seek 1000ml,26:53 @08:42:19.95 Cartridge 39 filtering 685/1000ml at 0.20ml/s with 31.2psi vacuum @08:42:23.24 Flow averaged 0.21ml/s over 10 seconds @08:42:23.47 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @08:42:24.08 Relieved excess vacuum in 1.3 seconds @08:42:24.15 SamplePump.seek 1000ml,26:44 @08:42:35.75 Flow averaged 0.21ml/s over 12 seconds @08:42:35.89 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @08:42:36.49 Relieved excess vacuum in .8 seconds @08:42:36.56 SamplePump.seek 1000ml,26:31 @08:42:43.40 Flow estimate reduced to 0.17ml/s @08:42:43.43 Intake.close @08:42:43.53 Exhaust.close @08:42:43.94 Toroid.in :hold @08:42:44.06 Toroid.seek :bypass @08:42:45.78 Toroid.in :default @08:42:45.92 Toroid.seek :clear @08:42:47.58 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:42:47.62 Pressure.stop @08:42:47.98 Sampled 689.3ml @08:42:48.69 Plunge.seek :reagent @08:42:49.54 Sample loop closed. Ready to Deliver Reagents @08:42:49.57 Archive.seek :sealed @08:42:51.15 Gate.power :sampler,:OFF @08:42:53.58 Archive.seek :evac @08:43:07.69 delay 1:00 @08:44:07.72 Archive.seek :clear @08:44:25.32 RNAlater soak for 10:00 @08:54:25.36 Archive.seek :tVent @08:54:27.86 delay 1 second @08:54:28.89 Archive.seek :evac @08:54:44.44 delay 10 seconds @08:54:54.48 Archive.seek :tVent @08:55:10.01 delay 1 second @08:55:11.03 Archive.seek :evac @08:55:26.60 delay 10 seconds @08:55:36.64 Sample preserved and evacuated @08:55:36.83 Selecting Cartridge 38 @08:55:37.12 Carousel.in :default @08:55:37.30 Carousel.home.seek 39 @08:55:37.71 Archive.in :unlocking @08:55:37.95 Archive.seek :clear @08:55:38.00 Air.in :unlocking @08:55:38.31 Air.seek :clear @08:55:42.67 Lysis1.in :unlocking @08:55:42.90 Lysis1.seek :clear @08:55:43.02 Plunge.in :unlocking @08:55:43.15 Plunge.seek :clear @08:55:44.62 Carousel.seek 38 @08:55:45.23 Lysis1.in :fast @08:55:45.34 Lysis1.seek :locked @08:55:48.01 Plunge.in :default @08:55:48.13 Plunge.seek :locked @08:55:48.35 Air.in :fast @08:55:48.45 Air.seek :locked @08:55:50.06 Carousel.reconfigure :locked @08:55:50.28 Powering cartridge bus @08:55:52.95 Gate.setIR 1750,5000,0,6 @08:55:53.09 Gate.setIR 0,5000,0,6 @08:55:53.19 PuckHeater.configure :default #ambient 27.4C @08:55:53.30 Gate.power :heat,:ON,limit to 5000mA @08:55:54.02 Mounted lysis Cartridge board#Q05034 @08:55:54.10 Using this cartridge's heat loss curve for HeatingAccelInCarousel @08:55:54.48 Preparing to filter cartridge 38 @08:55:54.77 Gate.power :sampler,:ON @08:55:54.91 Priming sample loop w/150ml, bypass w/50ml @08:55:54.93 SamplePump.setPosition! 0ml @08:55:55.04 Bypass.open @08:55:56.60 Intake.open @08:55:57.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:55:59.98 Vacuum tare is 56% of full scale @08:56:00.20 Pressure.in :default @08:56:00.31 Pressure.hold @08:56:00.59 Waiting for external sampler to finish priming... @08:56:00.60 delayUntil "08:59:24.79" @08:59:20.98 Session Begun @08:59:24.86 SamplePump.in :prime @08:59:25.01 SamplePump.seek 50ml,2:53 @09:00:05.69 -> Can Can@08:59:24, 24.8C, 42% humidity, 14.4psia, 14.852V, 0.413A, 3.948Ah, 6.14W @09:00:26.05 Pressure.stop @09:00:26.18 SamplePump.setPosition! 0ml @09:00:26.81 Exhaust.open @09:00:28.33 Bypass.close @09:00:29.99 Pressure.in :default @09:00:30.12 Pressure.hold @09:00:30.52 SamplePump.in :prime @09:00:30.66 SamplePump.seek 150ml,7:39 @09:00:36.40 -> Can Can@08:59:24, 24.8C, 42% humidity, 14.4psia, 14.852V, 0.413A, 3.948Ah, 6.14W @09:00:49.26 -> Can Can@09:00:48, 24.9C, 42% humidity, 14.4psia, 14.809V, 1.007A, 3.970Ah, 14.92W @09:03:33.02 Pressure.stop @09:03:33.17 Bypass.open @09:03:33.94 Intake.close @09:03:34.78 Toroid.in :hold @09:03:34.92 Toroid.seek :crack @09:03:36.52 Toroid.seek :cartridge @09:03:37.69 Toroid.in :default @09:03:37.86 Toroid.seek :clear @09:03:39.97 Pressurizing cartridge to 26psi above 13.2psia @09:03:40.01 SamplePump.setPosition! 0ml @09:03:40.13 Intake.open @09:03:40.84 Bypass.close @09:03:41.61 Exhaust.close @09:03:42.48 Pressure.in :default @09:03:42.61 Pressure.hold @09:03:42.92 SamplePump.in :charge @09:03:43.06 SamplePump.seek 2.3ml,42 seconds @09:03:56.86 Pressure.stop @09:03:56.98 Intake.close @09:03:57.79 Pressurized cartridge with 0.858ml to 26.0psi @09:03:57.82 Isolated @33.1psia (19.9psi) @09:03:57.88 Verifying cartridge holds at least 0.65..0.85 of 33.1psia @09:03:57.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:04:02.60 Cartridge pressure fell 4% to 31.9psia @09:04:02.76 Sampling 50.0ml @09:04:02.79 SamplePump.setPosition! 0ml @09:04:02.94 Exhaust.open @09:04:03.67 Intake.open @09:04:04.56 Pressure.in :default @09:04:04.69 Pressure.hold @09:04:05.44 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @09:04:05.71 SamplePump.in :sample @09:04:05.89 SamplePump.seek 50ml,4:41 @09:04:08.84 Cartridge 38 filtering 1/50ml at 0.55ml/s with 18.3psi vacuum @09:05:08.83 Cartridge 38 filtering 35/50ml at 0.56ml/s with 26.6psi vacuum @09:05:37.54 Pressure.stop @09:05:37.76 Intake.close @09:05:37.86 Exhaust.close @09:05:38.23 Toroid.in :hold @09:05:38.36 Toroid.seek :bypass @09:05:40.06 Toroid.in :default @09:05:40.20 Toroid.seek :clear @09:05:41.88 Sampled 50.0ml @09:05:43.66 SPR.command "read_sequences" @09:05:43.81 SPR.command "stats_on" @09:05:44.04 SPR.command "start 0 1" @09:05:44.73 SPR.start /var/log/esp/spr23aug1009h.spr.bz2 @09:05:45.07 Gate.power :sampler,:OFF @09:05:46.60 Handoff.select :waste @09:05:53.39 Syringe tare is 18% of full scale @09:05:53.43 PinPort.in :locking @09:05:53.59 PinPort.seek :locked @09:05:56.21 Archive.in :fast @09:05:56.36 Lysis2.in :fast @09:05:56.45 Lysis1.seek 1350uL @09:05:56.53 Archive.seek :full @09:05:56.82 Lysis2.seek 950uL @09:06:02.09 Plunge.seek :reagent @09:06:03.22 Evacuating puck with filter bubble point of 50psi @09:06:03.26 PinPort.seek :locked @09:06:03.46 Handoff.select :waste @09:06:10.08 Air syringe pumping up to 1 strokes to reach 8psi @09:06:10.10 Air stroke #1 of 1 @09:06:10.30 Air.in :pressurize @09:06:10.43 Air.seek :pressurized @09:06:16.24 2.8psi after pressurize @09:06:16.28 Air.in :compress @09:06:16.48 Air.seek :evac @09:06:19.58 3.3psi after compress @09:06:32.73 0.8psi after compress (stable) @09:06:32.75 Air.in :default @09:06:32.92 Air.hold @09:06:33.23 at 0.8psi Opened to waste @09:06:33.25 Handoff.select :blocked @09:06:33.52 Archive syringe pumping up to 8 strokes to reach 50psi @09:06:33.54 Archive stroke #1 of 8 @09:06:33.72 Archive.in :pressurize @09:06:33.89 Archive.seek :pressurized @09:06:46.45 1.3psi after pressurize @09:06:52.05 1.4psi after pressurize (stable) @09:06:52.12 Archive.in :compress @09:06:52.25 Archive.seek :empty @09:07:06.97 15.2psi after compress @09:07:17.57 16.3psi after compress (stable) @09:07:17.65 Archive.in :gulp @09:07:17.79 Archive.seek :topVent @09:07:24.16 16.4psi after gulp @09:07:29.76 16.4psi after gulp (stable) @09:07:29.79 Archive stroke #2 of 8 @09:07:30.00 Archive.in :pressurize @09:07:30.17 Archive.seek :pressurized @09:07:42.75 16.3psi after pressurize @09:07:48.35 16.2psi after pressurize (stable) @09:07:48.43 Archive.in :compress @09:07:48.56 Archive.seek :empty @09:08:03.28 27.9psi after compress @09:08:13.91 29.1psi after compress (stable) @09:08:13.95 Archive.in :gulp @09:08:14.12 Archive.seek :topVent @09:08:20.44 29.1psi after gulp @09:08:26.05 29.0psi after gulp (stable) @09:08:26.07 Archive stroke #3 of 8 @09:08:26.28 Archive.in :pressurize @09:08:26.41 Archive.seek :pressurized @09:08:38.96 28.9psi after pressurize @09:08:44.57 28.8psi after pressurize (stable) @09:08:44.64 Archive.in :compress @09:08:44.76 Archive.seek :empty @09:08:59.48 40.7psi after compress @09:09:10.08 41.6psi after compress (stable) @09:09:10.16 Archive.in :gulp @09:09:10.30 Archive.seek :topVent @09:09:16.64 41.7psi after gulp @09:09:22.24 41.7psi after gulp (stable) @09:09:22.26 Archive stroke #4 of 8 @09:09:22.46 Archive.in :pressurize @09:09:22.59 Archive.seek :pressurized @09:09:35.17 41.6psi after pressurize @09:09:40.77 41.4psi after pressurize (stable) @09:09:40.84 Archive.in :compress @09:09:40.96 Archive.seek :empty @09:09:53.32 Reached 50.1 of 50.0psi after pumping 6869uL (3.97 strokes) @09:09:53.35 Archive.in :default @09:09:53.51 Archive.hold @09:09:53.66 Handoff.select :waste @09:10:06.93 delay 1:00 @09:11:06.95 Handoff.select :blocked @09:11:29.73 Handoff.select :waste @09:11:29.95 0.5psi at evacEnd @09:11:29.96 Handoff.select :blocked @09:11:30.19 Archive.in :fast @09:11:30.33 Archive.seek :topVent @09:11:43.94 Stable @0.6psi after Handoff blocked @09:11:43.98 Archive syringe pumping up to 8 strokes to reach 31psi @09:11:44.05 Archive stroke #1 of 8 @09:11:44.23 Archive.in :pressurize @09:11:44.37 Archive.seek :pressurized @09:11:56.99 0.7psi after pressurize @09:11:57.03 Archive.in :compress @09:11:57.20 Archive.seek :empty @09:12:11.92 13.4psi after compress @09:12:22.52 13.8psi after compress (stable) @09:12:22.56 Archive.in :gulp @09:12:22.75 Archive.seek :topVent @09:12:28.00 Session Begun @09:12:29.15 13.9psi after gulp @09:12:34.65 -> showlog -200 @09:12:35.27 13.8psi after gulp (stable) @09:12:35.32 Archive stroke #2 of 8 @09:12:36.03 Archive.in :pressurize @09:12:36.28 Archive.seek :pressurized @09:12:48.90 13.7psi after pressurize @09:12:49.01 Archive.in :compress @09:12:49.16 Archive.seek :empty @09:13:03.92 24.7psi after compress @09:13:14.52 25.5psi after compress (stable) @09:13:14.55 Archive.in :gulp @09:13:14.76 Archive.seek :topVent @09:13:21.17 25.6psi after gulp @09:13:26.81 25.6psi after gulp (stable) @09:13:26.83 Archive stroke #3 of 8 @09:13:27.05 Archive.in :pressurize @09:13:27.20 Archive.seek :pressurized @09:13:39.78 25.5psi after pressurize @09:13:39.92 Archive.in :compress @09:13:40.10 Archive.seek :empty @09:13:48.59 Reached 30.8 of 30.6psi after pumping 5054uL (2.92 strokes) @09:13:48.61 Archive.in :default @09:13:48.95 Archive.hold @09:13:49.12 Handoff.select :waste @09:14:03.41 delay 1:00 @09:15:03.43 Handoff.select :blocked @09:15:26.23 Handoff.select :waste @09:15:26.45 0.7psi at evacEnd @09:15:26.52 Air.seek :fullVent @09:15:28.06 Archive.in :fast @09:15:28.20 Archive.seek :topVent @09:15:34.92 Seawater evacuated, Pressure above/below filter released. @09:15:35.10 Lysis1.in :slow @09:15:35.25 Lysis1.seek 1050uL @09:16:05.34 delay 5 seconds @09:16:10.37 Handoff.select :blocked @09:16:10.64 Lysis1.seek 450uL @09:17:10.67 delay 3 seconds @09:17:13.87 Air.in mix with 1.40048 parts Lysis1 @09:17:14.67 Air.seek :evac @09:17:14.73 Lysis1.seek 300uL @09:17:30.02 Lysis1.seek :empty @09:18:00.23 Lysis buffer delivered @10.2psi @09:18:00.27 Polling Can every 2:00 @09:18:00.95 PuckHeater.configure :default #ambient 25.4C @09:18:01.12 PuckHeater.seek 80.5C @09:18:56.33 PuckHeater.hold 2:00,80.5C @09:20:56.66 Polling Can every 2:00 @09:20:57.22 PuckHeater.configure :default #ambient 25.5C @09:20:57.36 PuckHeater.hold 30 seconds,79.5C @09:21:27.65 Polling Can every 2:00 @09:21:28.21 PuckHeater.configure :default #ambient 25.5C @09:21:28.36 PuckHeater.hold 30 seconds,78.9C @09:21:58.65 Polling Can every 2:00 @09:21:59.21 PuckHeater.configure :default #ambient 25.4C @09:21:59.38 PuckHeater.hold 10:00,78.4C @09:32:00.28 Lysis complete. Syringe pressure @13.0psi @09:32:00.31 Archive syringe pumping up to 5 strokes to reach 40psi @09:32:00.37 Archive stroke #1 of 5 @09:32:00.58 Archive.in :pressurize @09:32:00.74 Archive.seek :pressurized @09:32:13.36 12.9psi after pressurize @09:32:19.00 12.8psi after pressurize (stable) @09:32:19.10 Archive.in :compress @09:32:19.24 Archive.seek :empty @09:32:31.90 Reached 41.6 of 40.0psi after pumping 1672uL (0.97 strokes) @09:32:31.92 Archive.in :default @09:32:32.16 Archive.hold @09:32:32.35 delay 2 seconds @09:32:34.38 Archive.in :fast @09:32:34.52 Archive.seek :full @09:32:41.56 Lysis1.seek 138uL @09:32:55.55 delay 1 second @09:32:56.58 Archive.seek :full @09:32:56.73 Lysis1.seek :empty @09:33:10.74 Handoff.select :blocked @09:33:11.00 Initial air pressure @41.8psi @09:33:11.14 Lysis1.in :extract @09:33:11.35 Lysis1.seek 600uL,nil @09:33:17.11 Initial 600uL lysate @16.0psi @09:33:17.15 Handoff.select :blocked @09:33:17.37 Initial air pressure @16.0psi @09:33:17.53 Lysis1.seek 825uL,nil @09:33:20.17 Archive syringe pumping up to 6 strokes to reach 17psi @09:33:20.19 Archive stroke #1 of 6 @09:33:20.54 Archive.in :pressurize @09:33:20.68 Archive.seek :pressurized @09:33:33.37 13.3psi after pressurize @09:33:48.62 Archive.in :default @09:33:48.70 Archive.hold @09:33:49.16 Recovered 825uL lysate @15.4psi @09:33:49.20 Air.in :halfSpeed @09:33:49.40 Air.seek :fullVent @09:33:51.73 Puck Top Vented, bottom @13.6psi @09:33:51.75 Handoff.select :waste @09:33:52.04 delay 5 seconds @09:33:57.13 Handoff open to waste, bottom @0.8psi @09:33:57.21 Air.in :default @09:33:57.42 Air.seek :evac @09:33:58.93 Lysis2.in :DAmix @09:33:59.18 Lysis1.in :DAmix @09:33:59.91 Lysis2.seek 700uL @09:34:00.08 Lysis1.seek 575uL @09:34:35.23 delay 5 seconds @09:34:40.26 Handoff.select SPR @09:34:40.47 SPR.command "start 2 14" @09:34:41.25 SPR stabilized @09:34:41.50 delay 10 seconds @09:34:51.53 SPR.command "start 2 0" @09:34:55.15 Waiting up to 11:00 for SPR to enter state 1 @09:36:53.23 SPR.command "hold_on" @09:36:55.13 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:36:55.92 Lysis2.seek 400uL @09:36:56.08 Lysis1.seek 275uL @09:37:38.18 delay 15 seconds @09:37:53.20 SPR.command "hold_off" @09:37:56.73 Waiting up to 38:00 for SPR to enter state 7 @09:40:44.80 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50.0, lysed: 825, spr: spr23aug1009h.spr.bz2}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0915h.spr.bz2}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @09:52:16.39 SPRsummary:-6.63e+03RIU,4e+09RIU,186RIU,60.1RIU,none,none,none after 2949s @10:03:46.83 SPR analysis completed @10:03:57.04 Ended log spr23aug1009h.spr.bz2 @10:03:57.07 SPR.command "stats_off" @10:03:57.88 Handoff.select :close @10:03:58.11 Gate.power :heat,:OFF @10:03:58.18 Gate.power :cartridge,:OFF @10:03:58.36 PinPort.in :unlocking @10:03:58.50 PinPort.seek :clear @10:04:00.50 Carousel.in :default @10:04:00.71 Carousel.home.seek 38 @10:04:01.13 Plunge.seek :locked @10:04:02.12 Archive.in :unlocking @10:04:02.32 Archive.seek :clear @10:04:02.51 Air.in :unlocking @10:04:02.76 Air.seek :clear @10:04:02.83 Lysis1.in :unlocking @10:04:03.18 Lysis1.seek :clear @10:04:03.26 Lysis2.in :unlocking @10:04:03.50 Lysis2.seek :clear @10:04:07.45 Gate.power :motor,:OFF @10:04:07.47 Gate.power :core,:OFF @10:04:07.55 Gate.power :analytic1,:OFF @10:04:07.64 Gate.power :analytic2,:OFF @10:04:07.70 Gate.power :main,:OFF @10:04:07.80 Safely stopped and ready to power off @10:05:27.50 Session Ended @10:23:34.58 -> upsync #<#::NoThread:0xb6ce6e00> @10:26:38.13 -> upsync #<#::NoThread:0xb6ce6e00> @10:29:44.36 -> upsync #<#::NoThread:0xb6ce6e00> @10:32:44.68 -> exit @10:32:44.71 Session Ended @10:32:59.66 Session Begun @10:33:07.61 -> upsync #<#::NoThread:0xb6ce6e00> @10:33:13.61 -> Can Can@10:32:30, 25.3C, 42% humidity, 14.4psia, 14.887V, 0.081A, 4.824Ah, 1.21W @10:33:43.29 -> # Hi Nadia :) @10:34:01.05 -> clients [ESP["ben"],ESP["nadia"],ESP["ben#2"]] @10:40:17.03 -> #Hey Benji! @11:51:05.13 Session Begun @11:53:25.08 -> Can Can@11:52:14, 24.9C, 42% humidity, 14.3psia, 14.887V, 0.081A, 4.931Ah, 1.20W @11:53:46.79 -> exit @11:53:46.81 Session Ended @12:26:35.09 Session Begun @12:26:40.73 -> showlog -300 @12:27:25.61 -> exit @12:27:25.64 Session Ended @13:13:54.69 Session Ended @13:14:53.34 Session Ended @14:10:01.43 -> exit @14:10:01.45 Session Ended @15:09:36.28 Session Begun @15:09:38.49 -> can Can@15:09:38, 23.9C, 43% humidity, 14.3psia, 14.875V, 0.088A, 5.198Ah, 1.31W @15:09:57.51 -> exit @15:09:57.53 Session Ended @17:55:56.14 Session Begun @17:55:59.92 -> can Can@17:55:59, 24.2C, 43% humidity, 14.3psia, 14.874V, 0.088A, 5.422Ah, 1.31W @17:57:03.44 -> exit @17:57:03.46 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @18:55:22.95 Session Begun @18:55:30.25 Session Begun @18:55:35.49 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1009h.spr.bz2}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0915h.spr.bz2}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 53..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @18:56:07.06 -> Cartridge.release 53 1 @18:56:40.07 -> processAll --options=extraDilutions:[1,1,1] :standard @18:56:40.12 SyntaxError in bill -- compile error bill:3: syntax error, unexpected tLABEL processAll --options=extraDilutions:[1,1,1] :standard ^ bill:3: syntax error, unexpected ':', expecting $end processAll --options=extraDilutions:[1,1,1] :standard ^ @18:57:46.18 -> exit @18:57:46.20 Session Ended @18:57:51.39 -> exit @18:57:51.41 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @18:58:48.41 Session Begun @18:59:21.78 -> showlog @19:00:01.67 -> showlog @19:01:41.88 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1009h.spr.bz2}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug0915h.spr.bz2}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @19:02:40.42 -> MainThread.exit # @19:02:40.51 ThreadError in bill -- killed thread @19:02:40.57 Session Ended @19:03:56.38PDT10-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @19:03:58.03 >BREAK< ESP3 Gateway boot v1.43 for core bus @19:03:58.06 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @19:04:01.50 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @19:04:02.14 Sea pressure (Abs) tare is 6% of full scale @19:04:11.19 Polling Can every 2:00 @19:04:11.24 Waiting for espclients... @19:04:30.57 Session Begun @19:05:12.02 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @19:05:41.14 -> processAll --options=extraDilutions:[1,1,1] :standard @19:05:41.18 SyntaxError in bill2 -- compile error bill2:2: syntax error, unexpected tLABEL processAll --options=extraDilutions:[1,1,1] :standard ^ bill2:2: syntax error, unexpected ':', expecting $end processAll --options=extraDilutions:[1,1,1] :standard ^ @19:05:57.70 -> exit @19:05:57.72 Session Ended @21:03:52.55 Session Begun @21:03:55.78 -> can Can@21:03:55, 24.9C, 43% humidity, 14.3psia, 14.870V, 0.088A, 5.676Ah, 1.31W @21:04:30.03 Session Begun @21:04:48.38 -> processAll '--help' 1 @21:05:22.30 -> Cart.standard [53] @21:05:37.61 -> exit @21:05:37.64 Session Ended @21:05:55.30 -> showlog 200 @21:06:31.29 -> processAll '--options=extraDilutions:[1,1,1] :standard' @21:06:31.38 Sea pressure (Abs) tare is 6% of full scale @21:06:31.40 Sampling default goal volumes @21:06:31.45 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @21:06:31.56 Gate.power :sampler,:OFF,limit to 3500mA @21:06:31.58 Gate.power :main,:ON,limit to 5000mA @21:06:31.63 Gate.power :analytic1,:ON,limit to 5000mA @21:06:31.66 Gate.power :analytic2,:ON,limit to 5000mA @21:06:31.90 Powering core bus @21:06:34.12 SPR Module 1, FW Rev. B, FPGA Rev. C @21:06:34.68 Archive.configure :default @21:06:34.81 Air.configure :default @21:06:34.90 Lysis1.configure :default @21:06:35.00 Lysis2.configure :default @21:06:35.12 Plunge.configure :default @21:06:35.25 SamplePump.configure :default @21:06:35.38 Toroid.configure :default @21:06:35.51 Carousel.configure :default @21:06:35.64 Pressure.configure :default @21:06:35.77 ExtraPump.configure :default @21:06:35.90 PinPort.configure :default @21:06:36.00 All elf controllers running firmware version 4.84 @21:06:36.04 Gate.power :motor,:ON,limit to 5000mA @21:06:36.22 Archive.home.jog 0 @21:06:37.17 PinPort.home.jog 0 @21:06:37.20 Carousel.home.jog 0 @21:06:37.25 Plunge.home.jog 0 @21:06:37.29 Lysis2.home.jog 0 @21:06:37.33 Air.home.jog 0 @21:06:37.38 Toroid.home.jog 0 @21:06:37.43 Lysis1.home.jog 0 @21:06:38.32 Archive.in :homing @21:06:38.61 Archive.jog 1389 @21:06:38.68 PinPort.in :homing @21:06:38.72 Plunge.in :homing @21:06:38.79 Lysis1.in :homing @21:06:38.84 Toroid.in :homing @21:06:38.90 Air.in :homing @21:06:38.95 Lysis2.in :homing @21:06:39.30 PinPort.jog -107 @21:06:39.34 Plunge.jog 1573 @21:06:39.40 Lysis1.jog 1388 @21:06:39.45 Toroid.jog 591 @21:06:39.49 Air.jog 1370 @21:06:39.53 Lysis2.jog 1397 @21:06:39.99 PinPort.setRawPosition! 0 @21:06:40.06 PinPort.seek :home @21:06:40.20 PinPort.in :default @21:06:40.22 Archive.setRawPosition! 50 @21:06:40.37 Archive.seek :home @21:06:40.68 Archive.in :default @21:06:40.88 Lysis1.setRawPosition! 50 @21:06:40.96 Lysis1.seek :home @21:06:41.10 Plunge.setRawPosition! 50 @21:06:41.16 Lysis2.setRawPosition! 50 @21:06:41.19 Air.setRawPosition! 50 @21:06:41.32 Plunge.seek :home @21:06:41.51 Lysis2.seek :home @21:06:41.53 Air.seek :home @21:06:41.82 Plunge.in :default @21:06:41.84 Lysis1.in :default @21:06:42.46 Toroid.setRawPosition! 0 @21:06:42.59 Lysis2.in :default @21:06:42.63 Air.in :default @21:06:42.77 Toroid.seek :home @21:06:42.95 Toroid.in :default @21:06:43.11 Toroid.seek :clear @21:06:44.77 Carousel.in :default @21:06:44.93 Carousel.home.seek 38 @21:06:45.27 Selecting Cartridge 53 @21:06:45.63 Carousel.seek 53 @21:06:49.06 Lysis1.in :fast @21:06:49.18 Lysis1.seek :locked @21:06:51.88 Plunge.seek :locked @21:06:52.08 Air.in :fast @21:06:52.17 Air.seek :locked @21:06:53.77 Carousel.reconfigure :locked @21:06:54.01 Powering cartridge bus @21:06:56.68 Gate.setIR 1750,5000,0,6 @21:06:56.82 Gate.setIR 0,5000,0,6 @21:06:56.92 PuckHeater.configure :default @21:06:57.01 Gate.power :heat,:ON,limit to 5000mA @21:06:57.69 Mounted lysis Cartridge @21:06:57.73 WARNING: Using default puck temperature sensor calibration @21:06:57.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:06:59.44 SPR.command "read_sequences" @21:06:59.51 SPR.command "stats_on" @21:06:59.69 SPR.command "start 0 1" @21:07:00.38 SPR.start /var/log/esp/spr23aug1021h.spr.bz2 @21:07:02.36 Waiting for baseline SPR stabilization @21:07:02.38 Handoff.select :waste @21:07:13.64 Syringe tare is 18% of full scale @21:07:13.68 PinPort.in :locking @21:07:13.82 PinPort.seek :locked @21:07:16.40 Archive.in :fast @21:07:16.51 Lysis2.in :fast @21:07:16.60 Lysis1.seek 1000uL @21:07:16.64 Archive.seek :full @21:07:16.86 Lysis2.seek 1000uL @21:07:21.41 delayUntil "21:17:01.89" @21:17:01.91 Lysis1.in :DAmix @21:17:02.12 Lysis2.in :DAmix @21:17:02.78 Lysis1.seek 750uL @21:17:02.83 Lysis2.seek 750uL @21:17:37.97 delay 5 seconds @21:17:42.99 Handoff.select SPR @21:17:43.17 SPR.command "start 2 14" @21:17:44.58 SPR stabilized @21:17:44.77 delay 10 seconds @21:17:54.79 SPR.command "start 2 0" @21:17:57.95 Waiting up to 11:00 for SPR to enter state 1 @21:19:53.13 Session Begun @21:19:55.21 -> showlog @21:19:55.59 SPR.command "hold_on" @21:19:57.00 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:19:57.68 Lysis1.seek 450uL @21:19:57.74 Lysis2.seek 450uL @21:20:39.91 delay 15 seconds @21:20:54.93 SPR.command "hold_off" @21:20:58.14 Waiting up to 38:00 for SPR to enter state 7 @21:35:18.74 SPRsummary:-37.7RIU,2.67e+09RIU,131RIU,2.67e+09RIU after 1793s @21:46:49.34 delay 10 seconds @21:46:59.37 SPR.command "start 2 0" @21:47:02.06 Waiting up to 11:00 for SPR to enter state 1 @21:48:59.70 SPR.command "hold_on" @21:49:01.61 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:49:02.30 Lysis1.seek 300uL @21:49:02.41 Lysis2.seek 300uL @21:49:23.52 delay 15 seconds @21:49:38.54 SPR.command "hold_off" @21:49:41.90 Waiting up to 38:00 for SPR to enter state 7 @22:14:46.82 Session Begun @22:14:49.85 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr23aug1021h.spr.bz2}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @22:15:31.72 delay 10 seconds @22:15:41.74 SPR.command "start 2 0" @22:15:44.42 Waiting up to 11:00 for SPR to enter state 1 @22:17:42.08 SPR.command "hold_on" @22:17:43.47 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:17:44.19 Lysis1.seek 150uL @22:17:44.24 Lysis2.seek 150uL @22:18:05.44 delay 15 seconds @22:18:20.46 SPR.command "hold_off" @22:18:23.86 Waiting up to 38:00 for SPR to enter state 7 @22:44:15.03 delay 10 seconds @22:44:25.05 SPR.command "start 2 0" @22:44:27.73 Waiting up to 11:00 for SPR to enter state 1 @22:46:25.40 SPR.command "hold_on" @22:46:26.79 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:46:27.45 Lysis1.seek 0uL @22:46:27.51 Lysis2.seek 0uL @22:46:48.70 delay 15 seconds @22:47:03.73 SPR.command "hold_off" @22:47:06.63 Waiting up to 38:00 for SPR to enter state 7 @23:12:57.25 SPR analysis completed @23:13:01.59 Ended log spr23aug1021h.spr.bz2 @23:13:01.62 SPR.command "stats_off" @23:13:02.90 Handoff.select :close @23:13:03.11 Gate.power :heat,:OFF @23:13:03.14 Gate.power :cartridge,:OFF @23:13:03.34 PinPort.in :unlocking @23:13:03.46 PinPort.seek :clear @23:13:05.44 Carousel.in :default @23:13:05.64 Carousel.home.seek 53 @23:13:06.06 Plunge.seek :locked @23:13:06.32 Archive.in :unlocking @23:13:06.55 Archive.seek :clear @23:13:06.62 Air.in :unlocking @23:13:06.92 Air.seek :clear @23:13:07.09 Lysis1.in :unlocking @23:13:07.35 Lysis1.seek :clear @23:13:07.42 Lysis2.in :unlocking @23:13:07.62 Lysis2.seek :clear @23:13:12.28 Gate.power :motor,:OFF @23:13:12.31 Gate.power :core,:OFF @23:13:12.39 Gate.power :analytic1,:OFF @23:13:12.44 Gate.power :analytic2,:OFF @23:13:12.49 Gate.power :main,:OFF @23:13:12.55 Safely stopped and ready to power off @23:13:13.12 Cartridge::None in brent -- Not enough standard cartridges remain @23:13:13.28 -> #not quite done yet @23:13:32.60 -> cd /var/log/esp @23:13:32.63 SyntaxError in bill -- compile error bill:2: unknown regexp options - lg @23:13:50.70 -> exit @23:13:50.73 Session Ended @23:14:48.47 -> MainThead.exit @23:14:48.52 NameError in bill#2 -- uninitialized constant MainThead @23:14:57.99 -> MainThread.exit # @23:14:58.11 ThreadError in bill#2 -- killed thread @23:14:58.21 Session Ended @23:14:58.26 Session Ended @23:15:12.32PDT10-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @23:15:14.04 >BREAK< ESP3 Gateway boot v1.43 for core bus @23:15:14.06 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @23:15:17.65 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @23:15:18.33 Sea pressure (Abs) tare is 6% of full scale @23:15:28.10 Polling Can every 2:00 @23:15:28.15 Waiting for espclients... @23:15:28.55 Session Begun @23:15:34.23 -> sowlog :sowlog @23:15:36.71 -> showlog @23:15:42.14 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @23:15:56.46 -> ESP.ready! @23:15:56.52 Gate.power :sampler,:OFF,limit to 3500mA @23:15:56.54 Gate.power :main,:ON,limit to 5000mA @23:15:56.57 Gate.power :analytic1,:ON,limit to 5000mA @23:15:56.61 Gate.power :analytic2,:ON,limit to 5000mA @23:15:56.79 Powering core bus @23:15:59.06 SPR Module 1, FW Rev. B, FPGA Rev. C @23:15:59.55 Archive.configure :default @23:15:59.67 Air.configure :default @23:15:59.75 Lysis1.configure :default @23:15:59.88 Lysis2.configure :default @23:16:00.00 Plunge.configure :default @23:16:00.11 SamplePump.configure :default @23:16:00.24 Toroid.configure :default @23:16:00.36 Carousel.configure :default @23:16:00.48 Pressure.configure :default @23:16:00.59 ExtraPump.configure :default @23:16:00.69 PinPort.configure :default @23:16:01.24 All elf controllers running firmware version 4.84 @23:16:01.29 Gate.power :motor,:ON,limit to 5000mA @23:16:01.64 Archive.home.jog 0 @23:16:02.39 PinPort.home.jog 0 @23:16:02.41 Carousel.home.jog 0 @23:16:02.46 Plunge.home.jog 0 @23:16:02.50 Lysis2.home.jog 0 @23:16:02.54 Lysis1.home.jog 0 @23:16:02.58 Air.home.jog 0 @23:16:02.61 Toroid.home.jog 0 @23:16:03.52 Archive.in :homing @23:16:03.85 Toroid.in :homing @23:16:03.93 Lysis2.in :homing @23:16:04.00 Air.in :homing @23:16:04.07 PinPort.in :homing @23:16:04.12 Plunge.in :homing @23:16:04.17 Archive.jog 1387 @23:16:04.23 Lysis1.in :homing @23:16:04.54 Toroid.jog 587 @23:16:04.61 Lysis2.jog 1390 @23:16:04.67 Air.jog 1361 @23:16:04.71 PinPort.jog -107 @23:16:04.76 Lysis1.jog 1386 @23:16:04.84 Plunge.jog 1565 @23:16:05.26 PinPort.setRawPosition! 0 @23:16:05.34 PinPort.seek :home @23:16:05.45 PinPort.in :default @23:16:05.62 Archive.setRawPosition! 50 @23:16:05.70 Archive.seek :home @23:16:06.00 Archive.in :default @23:16:06.16 Air.setRawPosition! 50 @23:16:06.17 Lysis2.setRawPosition! 50 @23:16:06.31 Air.seek :home @23:16:06.40 Lysis2.seek :home @23:16:06.58 Lysis1.setRawPosition! 50 @23:16:06.61 Plunge.setRawPosition! 50 @23:16:06.73 Lysis1.seek :home @23:16:06.79 Plunge.seek :home @23:16:07.00 Toroid.setRawPosition! 0 @23:16:07.06 Air.in :default @23:16:07.12 Lysis2.in :default @23:16:07.27 Toroid.seek :home @23:16:07.47 Plunge.in :default @23:16:07.50 Lysis1.in :default @23:16:07.68 Toroid.in :default @23:16:07.94 Toroid.seek :clear @23:16:09.59 Carousel.in :default @23:16:09.74 Carousel.home.seek 53 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 53 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @23:16:22.15 -> SPR.start @23:16:23.33 SPR.command "read_sequences" @23:16:23.50 SPR.command "stats_on" # @23:16:24.37 SPR.start /var/log/esp/spr23aug1023h.spr.bz2 @23:16:41.26 -> SPR.command "start 3 0" @23:16:41.29 SPR.command "start 3 0" @00:03:05.42PDT11-Aug-23 -> SPR.command "start 2 14" @00:03:05.45 SPR.command "start 2 14" @00:03:14.07 -> SPR.stop @00:03:17.28 Ended log spr23aug1023h.spr.bz2 @00:03:17.30 SPR.command "stats_off" @00:32:15.49 -> Cmd.stop @00:32:15.71 Carousel.in :default @00:32:15.91 Carousel.home.seek 53 @00:32:16.28 Lysis1.in :fast @00:32:16.37 Lysis1.seek :locked @00:32:18.91 Plunge.seek :locked @00:32:20.30 Lysis1.in :unlocking @00:32:20.48 Lysis1.seek :clear @00:32:22.01 Gate.power :motor,:OFF @00:32:22.04 Gate.power :core,:OFF @00:32:22.07 Gate.power :analytic1,:OFF @00:32:22.11 Gate.power :analytic2,:OFF @00:32:22.14 Gate.power :main,:OFF @00:32:22.19 Safely stopped and ready to power off :STOPPED @00:32:33.98 -> exit @00:32:34.00 Session Ended @06:39:09.45 Session Begun @06:39:15.23 -> Can Can@06:37:57, 24.7C, 43% humidity, 14.4psia, 14.885V, 0.082A, 7.976Ah, 1.22W @06:42:23.91 -> exit @06:42:23.94 Session Ended @06:42:54.06 Session Begun @06:49:41.15 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @06:55:19.15 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @06:55:19.38 Gate.power :sampler,:OFF,limit to 3500mA @06:55:19.43 Gate.power :main,:ON,limit to 5000mA @06:55:19.47 Gate.power :analytic1,:ON,limit to 5000mA @06:55:19.51 Gate.power :analytic2,:ON,limit to 5000mA @06:55:19.60 Powering core bus @06:55:21.95 SPR Module 1, FW Rev. B, FPGA Rev. C @06:55:22.38 Archive.configure :default @06:55:22.51 Air.configure :default @06:55:22.60 Lysis1.configure :default @06:55:22.69 Lysis2.configure :default @06:55:22.81 Plunge.configure :default @06:55:22.94 SamplePump.configure :default @06:55:23.07 Toroid.configure :default @06:55:23.19 Carousel.configure :default @06:55:23.30 Pressure.configure :default @06:55:23.42 ExtraPump.configure :default @06:55:23.51 PinPort.configure :default @06:55:24.08 All elf controllers running firmware version 4.84 @06:55:24.23 Gate.power :motor,:ON,limit to 5000mA @06:55:24.68 Archive.home.jog 0 @06:55:25.31 Carousel.home.jog 0 @06:55:25.36 Toroid.home.jog 0 @06:55:25.40 Lysis2.home.jog 0 @06:55:25.45 Air.home.jog 0 @06:55:25.51 PinPort.home.jog 0 @06:55:25.55 Plunge.home.jog 0 @06:55:25.61 Lysis1.home.jog 0 @06:55:26.45 Archive.in :homing @06:55:26.78 Archive.jog 1383 @06:55:27.08 PinPort.in :homing @06:55:27.15 Toroid.in :homing @06:55:27.19 Lysis2.in :homing @06:55:27.25 Air.in :homing @06:55:27.32 Plunge.in :homing @06:55:27.38 Lysis1.in :homing @06:55:27.66 Toroid.jog 610 @06:55:27.73 PinPort.jog -106 @06:55:27.77 Lysis2.jog 1390 @06:55:27.86 Plunge.jog 1563 @06:55:27.89 Lysis1.jog 1383 @06:55:27.93 Air.jog 1363 @06:55:28.41 PinPort.setRawPosition! 0 @06:55:28.43 Archive.setRawPosition! 50 @06:55:28.56 Archive.seek :home @06:55:28.62 PinPort.seek :home @06:55:28.83 PinPort.in :default @06:55:28.98 Archive.in :default @06:55:29.30 Lysis2.setRawPosition! 50 @06:55:29.45 Lysis1.setRawPosition! 50 @06:55:29.49 Air.setRawPosition! 50 @06:55:29.53 Lysis2.seek :home @06:55:29.58 Plunge.setRawPosition! 50 @06:55:29.86 Plunge.seek :home @06:55:29.90 Air.seek :home @06:55:29.96 Lysis1.seek :home @06:55:30.24 Toroid.setRawPosition! 0 @06:55:30.27 Lysis2.in :default @06:55:30.44 Toroid.seek :home @06:55:30.60 Plunge.in :default @06:55:30.64 Lysis1.in :default @06:55:30.68 Toroid.in :default @06:55:30.75 Air.in :default @06:55:31.28 Toroid.seek :clear @06:55:32.92 Carousel.in :default @06:55:33.08 Carousel.home.seek 53 @06:55:33.38 Selecting Cartridge 37 @06:55:33.72 Carousel.seek 37 @06:55:37.35 Lysis1.in :fast @06:55:37.45 Lysis1.seek :locked @06:55:40.50 Plunge.seek :locked @06:55:40.84 Air.in :fast @06:55:40.99 Air.seek :locked @06:55:42.60 Carousel.reconfigure :locked @06:55:42.80 Powering cartridge bus @06:55:45.46 Gate.setIR 1750,5000,0,6 @06:55:45.59 Gate.setIR 0,5000,0,6 @06:55:45.69 Gate.power :cartridge,:OFF @06:55:46.25 Mounted archive Cartridge @06:55:46.62 Preparing to filter cartridge 37 @06:55:46.90 Gate.power :sampler,:ON @06:55:47.02 Priming sample loop w/150ml, bypass w/50ml @06:55:47.05 SamplePump.setPosition! 0ml @06:55:47.14 Bypass.open @06:55:48.70 Intake.open @06:55:49.49 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:55:52.08 Vacuum tare is 56% of full scale @06:55:52.29 Pressure.in :default @06:55:52.38 Pressure.hold @06:55:52.64 Waiting for external sampler to finish priming... @06:55:52.66 delayUntil "06:59:16.92" @06:59:16.97 SamplePump.in :prime @06:59:17.08 SamplePump.seek 50ml,2:53 @06:59:44.36 -> Can Can@06:59:16, 24.7C, 43% humidity, 14.4psia, 14.852V, 0.363A, 8.029Ah, 5.39W @07:00:18.11 Pressure.stop @07:00:18.19 SamplePump.setPosition! 0ml @07:00:18.27 Exhaust.open @07:00:19.74 Bypass.close @07:00:21.39 Pressure.in :default @07:00:21.49 Pressure.hold @07:00:21.86 SamplePump.in :prime @07:00:21.96 SamplePump.seek 150ml,7:39 @07:03:24.33 Pressure.stop @07:03:24.44 Bypass.open @07:03:25.22 Intake.close @07:03:26.02 Toroid.in :hold @07:03:26.12 Toroid.seek :crack @07:03:27.68 Toroid.seek :cartridge @07:03:28.82 Toroid.in :default @07:03:28.91 Toroid.seek :clear @07:03:30.96 Pressurizing cartridge to 26psi above 13.6psia @07:03:30.99 SamplePump.setPosition! 0ml @07:03:31.06 Intake.open @07:03:32.26 Bypass.close @07:03:33.02 Exhaust.close @07:03:33.94 Pressure.in :default @07:03:34.03 Pressure.hold @07:03:34.31 SamplePump.in :charge @07:03:34.41 SamplePump.seek 2.3ml,42 seconds @07:03:41.87 Pressure.stop @07:03:41.96 Intake.close @07:03:42.74 Pressurized cartridge with 0.245ml to 26.6psi @07:03:42.76 Isolated @34.5psia (20.9psi) @07:03:42.78 Verifying cartridge holds at least 0.65..0.85 of 34.5psia @07:03:42.84 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:03:48.47 Cartridge pressure fell 9% to 31.4psia @07:03:48.60 Sampling 1000.0ml @07:03:48.61 SamplePump.setPosition! 0ml @07:03:48.70 Exhaust.open @07:03:49.49 Intake.open @07:03:50.34 Pressure.in :default @07:03:50.44 Pressure.hold @07:03:50.61 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:03:50.83 SamplePump.in :sample @07:03:50.94 SamplePump.seek 1000ml,1:23:51 @07:03:53.98 Cartridge 37 filtering 1/1000ml at 0.62ml/s with 23.0psi vacuum @07:04:53.97 Cartridge 37 filtering 31/1000ml at 0.50ml/s with 19.1psi vacuum @07:05:53.97 Cartridge 37 filtering 57/1000ml at 0.38ml/s with 15.0psi vacuum @07:06:53.96 Cartridge 37 filtering 79/1000ml at 0.36ml/s with 16.2psi vacuum @07:07:53.97 Cartridge 37 filtering 99/1000ml at 0.28ml/s with 24.1psi vacuum @07:08:53.96 Cartridge 37 filtering 115/1000ml at 0.31ml/s with 25.7psi vacuum @07:09:48.74 Flow averaged 0.36ml/s over 5:58 @07:09:48.81 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @07:09:49.42 Relieved excess vacuum in .7 seconds @07:09:49.47 SamplePump.seek 1000ml,1:13:15 @07:09:53.98 Cartridge 37 filtering 128/1000ml at 0.20ml/s with 22.5psi vacuum @07:10:05.13 Flow averaged 0.22ml/s over 16 seconds @07:10:05.20 Waiting up to 14 seconds for vacuum to reduce from 28.4 to 18.0psi @07:10:05.84 Relieved excess vacuum in .7 seconds @07:10:06.34 SamplePump.seek 1000ml,1:12:59 @07:10:17.91 Flow averaged 0.21ml/s over 13 seconds @07:10:17.99 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @07:10:18.60 Relieved excess vacuum in .7 seconds @07:10:18.66 SamplePump.seek 1000ml,1:12:46 @07:10:27.29 Flow estimate reduced to 0.17ml/s @07:10:27.31 Intake.close @07:10:27.40 Exhaust.close @07:10:27.72 Toroid.in :hold @07:10:27.84 Toroid.seek :bypass @07:10:29.53 Toroid.in :default @07:10:29.63 Toroid.seek :clear @07:10:31.28 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @07:10:31.31 Pressure.stop @07:10:31.60 Sampled 134.8ml @07:10:32.33 Plunge.seek :reagent @07:10:33.17 Sample loop closed. Ready to Deliver Reagents @07:10:33.20 Archive.seek :sealed @07:10:34.75 Gate.power :sampler,:OFF @07:10:37.19 Archive.seek :evac @07:10:51.25 delay 1:00 @07:11:51.29 Archive.seek :clear @07:12:08.81 RNAlater soak for 10:00 @07:22:08.84 Archive.seek :tVent @07:22:11.32 delay 1 second @07:22:12.35 Archive.seek :evac @07:22:27.89 delay 10 seconds @07:22:37.93 Archive.seek :tVent @07:22:53.48 delay 1 second @07:22:54.51 Archive.seek :evac @07:23:10.06 delay 10 seconds @07:23:20.10 Sample preserved and evacuated @07:23:20.27 Selecting Cartridge 36 @07:23:20.54 Carousel.in :default @07:23:20.70 Carousel.home.seek 37 @07:23:21.09 Archive.in :unlocking @07:23:21.29 Archive.seek :clear @07:23:21.39 Air.in :unlocking @07:23:21.58 Air.seek :clear @07:23:25.95 Lysis1.in :unlocking @07:23:26.15 Lysis1.seek :clear @07:23:26.22 Plunge.in :unlocking @07:23:26.37 Plunge.seek :clear @07:23:27.81 Carousel.seek 36 @07:23:28.87 Lysis1.in :fast @07:23:29.00 Lysis1.seek :locked @07:23:31.66 Plunge.in :default @07:23:31.81 Plunge.seek :locked @07:23:31.98 Air.in :fast @07:23:32.08 Air.seek :locked @07:23:33.68 Carousel.reconfigure :locked @07:23:33.92 Powering cartridge bus @07:23:36.59 Gate.setIR 1750,5000,0,6 @07:23:36.72 Gate.setIR 0,5000,0,6 @07:23:36.81 PuckHeater.configure :default @07:23:36.90 Gate.power :heat,:ON,limit to 5000mA @07:23:37.66 Mounted lysis Cartridge board#Q05045 @07:23:37.73 Using default cartridge heat loss curve for HeatingAccelInCarousel @07:23:38.14 Preparing to filter cartridge 36 @07:23:38.42 Gate.power :sampler,:ON @07:23:38.54 Priming sample loop w/150ml, bypass w/50ml @07:23:38.56 SamplePump.setPosition! 0ml @07:23:38.64 Bypass.open @07:23:40.17 Intake.open @07:23:40.98 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:23:43.57 Vacuum tare is 56% of full scale @07:23:43.77 Pressure.in :default @07:23:43.91 Pressure.hold @07:23:44.17 Waiting for external sampler to finish priming... @07:23:44.20 delayUntil "07:27:08.44" @07:27:08.49 SamplePump.in :prime @07:27:08.60 SamplePump.seek 50ml,2:53 @07:28:09.62 Pressure.stop @07:28:09.71 SamplePump.setPosition! 0ml @07:28:09.78 Exhaust.open @07:28:11.29 Bypass.close @07:28:12.89 Pressure.in :default @07:28:12.99 Pressure.hold @07:28:13.34 SamplePump.in :prime @07:28:13.44 SamplePump.seek 150ml,7:39 @07:31:15.84 Pressure.stop @07:31:15.94 Bypass.open @07:31:16.68 Intake.close @07:31:17.53 Toroid.in :hold @07:31:17.62 Toroid.seek :crack @07:31:19.20 Toroid.seek :cartridge @07:31:20.44 Toroid.in :default @07:31:20.54 Toroid.seek :clear @07:31:22.59 Pressurizing cartridge to 26psi above 13.6psia @07:31:22.61 SamplePump.setPosition! 0ml @07:31:22.70 Intake.open @07:31:23.40 Bypass.close @07:31:24.15 Exhaust.close @07:31:25.01 Pressure.in :default @07:31:25.11 Pressure.hold @07:31:25.38 SamplePump.in :charge @07:31:25.48 SamplePump.seek 2.3ml,42 seconds @07:31:39.00 Pressure.stop @07:31:39.09 Intake.close @07:31:39.93 Pressurized cartridge with 0.823ml to 26.1psi @07:31:39.94 Isolated @33.8psia (20.2psi) @07:31:39.97 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @07:31:40.03 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:31:44.65 Cartridge pressure fell 2% to 33.3psia @07:31:44.78 Sampling 50.0ml @07:31:44.80 SamplePump.setPosition! 0ml @07:31:44.89 Exhaust.open @07:31:45.62 Intake.open @07:31:46.50 Pressure.in :default @07:31:46.59 Pressure.hold @07:31:46.76 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:31:46.98 SamplePump.in :sample @07:31:47.08 SamplePump.seek 50ml,4:41 @07:31:50.12 Cartridge 36 filtering 1/50ml at 0.48ml/s with 26.9psi vacuum @07:32:50.12 Cartridge 36 filtering 28/50ml at 0.43ml/s with 23.7psi vacuum @07:33:48.63 Pressure.stop @07:33:48.81 Intake.close @07:33:48.86 Exhaust.close @07:33:49.18 Toroid.in :hold @07:33:49.28 Toroid.seek :bypass @07:33:50.94 Toroid.in :default @07:33:51.04 Toroid.seek :clear @07:33:52.68 Sampled 50.0ml @07:33:54.96 SPR.command "read_sequences" @07:33:55.32 SPR.command "stats_on" @07:33:55.52 SPR.command "start 0 1" @07:33:55.84 Gate.power :sampler,:OFF @07:33:56.21 SPR.start /var/log/esp/spr23aug1107h.spr.bz2 @07:33:58.17 Handoff.select :waste @07:34:04.92 Syringe tare is 18% of full scale @07:34:04.95 PinPort.in :locking @07:34:05.07 PinPort.seek :locked @07:34:07.64 Archive.in :fast @07:34:07.76 Lysis2.in :fast @07:34:07.88 Lysis1.seek 1350uL @07:34:07.92 Archive.seek :full @07:34:08.15 Lysis2.seek 950uL @07:34:12.91 Plunge.seek :reagent @07:34:13.93 Evacuating puck with filter bubble point of 50psi @07:34:13.96 PinPort.seek :locked @07:34:14.12 Handoff.select :waste @07:34:20.73 Air syringe pumping up to 1 strokes to reach 8psi @07:34:20.75 Air stroke #1 of 1 @07:34:20.89 Air.in :pressurize @07:34:20.99 Air.seek :pressurized @07:34:26.76 0.7psi after pressurize @07:34:26.79 Air.in :compress @07:34:26.90 Air.seek :evac @07:34:29.97 1.0psi after compress @07:34:40.57 0.7psi after compress (stable) @07:34:40.59 Air.in :default @07:34:40.70 Air.hold @07:34:40.95 at 0.4psi Opened to waste @07:34:40.98 Handoff.select :blocked @07:34:41.17 Archive syringe pumping up to 8 strokes to reach 50psi @07:34:41.19 Archive stroke #1 of 8 @07:34:41.35 Archive.in :pressurize @07:34:41.46 Archive.seek :pressurized @07:34:54.00 1.6psi after pressurize @07:34:59.60 1.6psi after pressurize (stable) @07:34:59.63 Archive.in :compress @07:34:59.73 Archive.seek :empty @07:35:14.42 15.0psi after compress @07:35:25.52 16.8psi after compress (stable) @07:35:25.55 Archive.in :gulp @07:35:25.70 Archive.seek :topVent @07:35:31.98 17.4psi after gulp @07:35:37.58 17.1psi after gulp (stable) @07:35:37.59 Archive stroke #2 of 8 @07:35:37.73 Archive.in :pressurize @07:35:37.86 Archive.seek :pressurized @07:35:50.39 17.1psi after pressurize @07:35:56.01 17.0psi after pressurize (stable) @07:35:56.05 Archive.in :compress @07:35:56.16 Archive.seek :empty @07:36:10.83 30.2psi after compress @07:36:29.44 26.9psi after compress (stable) @07:36:29.47 Archive.in :gulp @07:36:29.58 Archive.seek :topVent @07:36:35.91 25.8psi after gulp @07:36:59.51 19.0psi after gulp (stable) @07:36:59.53 Archive stroke #3 of 8 @07:36:59.68 Archive.in :pressurize @07:36:59.77 Archive.seek :pressurized @07:37:12.34 17.8psi after pressurize @07:37:17.94 17.8psi after pressurize (stable) @07:37:17.97 Archive.in :compress @07:37:18.08 Archive.seek :empty @07:37:32.74 26.1psi after compress @07:37:52.97 21.4psi after compress (stable) @07:37:53.01 Archive.in :gulp @07:37:53.11 Archive.seek :topVent @07:37:59.43 17.3psi after gulp @07:38:05.03 16.7psi after gulp (stable) @07:38:05.05 Archive stroke #4 of 8 @07:38:05.19 Archive.in :pressurize @07:38:05.29 Archive.seek :pressurized @07:38:18.00 14.3psi after pressurize @07:38:30.12 16.7psi after pressurize (stable) @07:38:30.15 Archive.in :compress @07:38:30.28 Archive.seek :empty @07:38:44.94 22.2psi after compress @07:39:28.05 14.2psi after compress (stable) @07:39:28.08 Archive.in :gulp @07:39:28.19 Archive.seek :topVent @07:39:34.53 13.8psi after gulp @07:39:40.15 13.5psi after gulp (stable) @07:39:40.18 Archive stroke #5 of 8 @07:39:40.33 Archive.in :pressurize @07:39:40.43 Archive.seek :pressurized @07:39:52.97 12.9psi after pressurize @07:40:05.70 16.4psi after pressurize (stable) @07:40:05.73 Archive.in :compress @07:40:05.87 Archive.seek :empty @07:40:20.54 22.4psi after compress @07:40:53.14 17.5psi after compress (stable) @07:40:53.17 Archive.in :gulp @07:40:53.29 Archive.seek :topVent @07:41:00.15 14.5psi after gulp @07:41:05.75 13.8psi after gulp (stable) @07:41:05.77 Archive stroke #6 of 8 @07:41:05.94 Archive.in :pressurize @07:41:06.04 Archive.seek :pressurized @07:41:18.58 13.3psi after pressurize @07:41:32.18 16.1psi after pressurize (stable) @07:41:32.21 Archive.in :compress @07:41:32.32 Archive.seek :empty @07:41:46.98 22.6psi after compress @07:42:12.09 19.7psi after compress (stable) @07:42:12.12 Archive.in :gulp @07:42:12.23 Archive.seek :topVent @07:42:18.53 16.4psi after gulp @07:42:37.63 11.9psi after gulp (stable) @07:42:37.64 Archive stroke #7 of 8 @07:42:37.81 Archive.in :pressurize @07:42:37.98 Archive.seek :pressurized @07:42:50.52 10.3psi after pressurize @07:43:05.12 13.9psi after pressurize (stable) @07:43:05.16 Archive.in :compress @07:43:05.28 Archive.seek :empty @07:43:19.97 20.3psi after compress @07:43:30.57 20.1psi after compress (stable) @07:43:30.61 Archive.in :gulp @07:43:30.72 Archive.seek :topVent @07:43:37.02 17.2psi after gulp @07:43:49.62 15.3psi after gulp (stable) @07:43:49.63 Archive stroke #8 of 8 @07:43:49.82 Archive.in :pressurize @07:43:49.95 Archive.seek :pressurized @07:44:02.50 13.3psi after pressurize @07:44:16.60 16.1psi after pressurize (stable) @07:44:16.63 Archive.in :compress @07:44:16.75 Archive.seek :empty @07:44:31.45 22.7psi after compress @07:45:04.58 18.5psi after compress (stable) @07:45:04.60 Archive.in :default @07:45:04.72 Archive.hold @07:45:04.91 Handoff.select :blocked @07:45:04.98 Underpressure in PROCESSING -- Reached only 18.2 of 50.0psi after pumping 13840uL @07:45:05.00 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @07:47:28.97 -> showlog -20 @07:50:24.66 -> Can Can@07:49:44, 25.7C, 42% humidity, 14.4psia, 14.858V, 0.463A, 8.629Ah, 6.87W @07:55:14.64 Ended log spr23aug1107h.spr.bz2 @07:55:14.66 SPR.command "stats_off" @07:55:29.42 Session Begun @07:55:45.95 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug1107h.spr.bz2}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @08:04:36.50 -> exit @08:04:36.53 Session Ended @08:10:55.20 -> showlog -20 @08:14:15.85 -> Power {analytic1: true, analytic2: true, cartridge: true, core: true, heat: true, main: true, motor: true, sampler: false} @08:27:21.82

Session Begun @08:27:29.84 -> Air Air Syringe (AirS) at evac @08:27:37.06 -> Handoff Handoff Valve::Manifold selects blocked @08:27:40.57 -> Syringe.psi 4.74705242692233 @08:28:19.22 -> Lysis @08:28:19.25 NameError in p -- uninitialized constant Lysis @08:28:21.36 -> Lysis1 Lysis Syringe #1 (LyS1) at 1350uL @08:28:23.92 -> Lysis2 Lysis Syringe #2 (LyS2) at 950uL @08:28:28.09 -> ArS Archive Syringe (ArS) empty @08:28:34.16 -> PP Handoff Pin Port (PP) cartridge @08:29:34.59 Session Begun @08:29:49.29

-> Syringe.pumpTo 20 @08:29:49.35 NoMethodError in p -- undefined method `pumpTo' for Syringe:Class @08:29:58.06 -> Air.pumpTo 20 @08:29:58.12 Air syringe pumping up to 8 strokes to reach 20psi @08:29:58.60 Air stroke #1 of 8 @08:29:58.84 Air.in :compress @08:29:58.97 Air.seek :evac @08:29:59.19 4.8psi after compress @08:30:09.81 4.8psi after compress (stable) @08:30:09.86 Air.in :gulp @08:30:10.01 Air.seek :topVent @08:30:15.93 4.8psi after gulp @08:30:21.54 4.9psi after gulp (stable) @08:30:21.56 Air stroke #2 of 8 @08:30:21.76 Air.in :pressurize @08:30:21.92 Air.seek :pressurized @08:30:26.12 -> Syringe.psi 4.92145267721854 @08:30:28.48

4.9psi after pressurize @08:30:28.52 Air.in :compress @08:30:28.67 Air.seek :evac @08:30:31.76 13.3psi after compress @08:30:34.35 -> Syringe.psi 15.2997973966001 @08:30:37.60 -> Syringe.psi 15.4680783398684 @08:30:42.86

15.5psi after compress (stable) @08:30:42.90 Air.in :gulp @08:30:43.06 Air.seek :topVent @08:30:46.88 -> Syringe.psi 15.2676710347035 @08:30:48.97

15.3psi after gulp @08:30:54.57 15.2psi after gulp (stable) @08:30:54.59 Air stroke #3 of 8 @08:30:54.89 Air.in :pressurize @08:30:55.03 Air.seek :pressurized @08:31:01.58 14.9psi after pressurize @08:31:01.60 Air.in :compress @08:31:01.76 Air.seek :evac @08:31:03.77 Reached 20.2 of 20.0psi after pumping 4716uL (2.96 strokes) @08:31:03.79 Air.in :default @08:31:04.00 Air.hold 4716.17092866756 @08:31:06.38 -> Syringe.psi 22.4440883517169 @08:31:17.86

-> Air.pumpTo 30 @08:31:17.92 Air syringe pumping up to 8 strokes to reach 30psi @08:31:17.96 Air stroke #1 of 8 @08:31:18.14 Air.in :compress @08:31:18.27 Air.seek :evac @08:31:19.62 22.7psi after compress @08:31:23.41 -> Syringe.psi 23.9188413454498 @08:31:30.42 -> SC Storage Carousel (SC) at 36 @08:31:30.73

24.0psi after compress (stable) @08:31:30.77 Air.in :gulp @08:31:30.94 Air.seek :topVent @08:31:33.91 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug1107h.spr.bz2}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @08:31:37.37

22.7psi after gulp @08:31:42.97 22.4psi after gulp (stable) @08:31:42.99 Air stroke #2 of 8 @08:31:43.20 Air.in :pressurize @08:31:43.33 Air.seek :pressurized @08:31:49.90 21.3psi after pressurize @08:31:49.97 Air.in :compress @08:31:50.10 Air.seek :evac @08:31:53.19 28.1psi after compress @08:31:54.92 Reached 30.1 of 30.0psi after pumping 3188uL (2.00 strokes) @08:31:54.94 Air.in :default @08:31:55.10 Air.hold 3187.57738896366 @08:33:54.80 -> Cartridge.sampler..evac2waste @08:33:54.84 ArgumentError in p2 -- bad value for range @08:34:02.08 -> Cartridge.sampler.evac2waste @08:34:02.10 Handoff.select :waste @08:34:08.44 -> Can Can@08:33:34, 25.9C, 42% humidity, 14.4psia, 14.856V, 0.474A, 8.983Ah, 7.04W @08:34:10.41 delay 1:00 @08:35:10.43 Handoff.select :blocked @08:35:33.20 Handoff.select :waste @08:35:33.38 0.3psi at evacEnd @08:37:16.64

-> Handoff.blocked @08:37:16.67 Handoff.select :blocked Handoff Valve::Manifold selects blocked @08:37:20.08 -> Air Air Syringe (AirS) at evac @08:37:25.28 -> Syringe.psi 0.246302107874477 @08:38:10.40 -> Air.pumpTo 30; Cartridge.sampler.evac2waste; Air.fVent @08:38:10.46 Air syringe pumping up to 8 strokes to reach 30psi @08:38:10.50 Air stroke #1 of 8 @08:38:10.69 Air.in :compress @08:38:10.82 Air.seek :evac @08:38:11.03 0.3psi after compress @08:38:21.63 0.3psi after compress (stable) @08:38:21.66 Air.in :gulp @08:38:21.84 Air.seek :topVent @08:38:27.73 0.6psi after gulp @08:38:33.33 0.6psi after gulp (stable) @08:38:33.35 Air stroke #2 of 8 @08:38:33.55 Air.in :pressurize @08:38:33.68 Air.seek :pressurized @08:38:40.24 2.3psi after pressurize @08:38:40.32 Air.in :compress @08:38:40.44 Air.seek :evac @08:38:43.54 10.0psi after compress @08:38:54.64 12.0psi after compress (stable) @08:38:54.72 Air.in :gulp @08:38:54.83 Air.seek :topVent @08:39:00.72 12.2psi after gulp @08:39:06.33 12.1psi after gulp (stable) @08:39:06.35 Air stroke #3 of 8 @08:39:06.53 Air.in :pressurize @08:39:06.66 Air.seek :pressurized @08:39:13.20 12.4psi after pressurize @08:39:13.24 Air.in :compress @08:39:13.41 Air.seek :evac @08:39:16.49 20.2psi after compress @08:39:27.09 22.1psi after compress (stable) @08:39:27.23 Air.in :gulp @08:39:27.33 Air.seek :topVent @08:39:33.30 21.1psi after gulp @08:39:38.92 21.0psi after gulp (stable) @08:39:38.94 Air stroke #4 of 8 @08:39:39.13 Air.in :pressurize @08:39:39.27 Air.seek :pressurized @08:39:42.75 -> exit @08:39:42.78 Session Ended @08:39:45.85

20.4psi after pressurize @08:39:45.88 Air.in :compress @08:39:46.00 Air.seek :evac @08:39:49.07 27.4psi after compress @08:39:59.67 29.4psi after compress (stable) @08:39:59.70 Air.in :gulp @08:39:59.87 Air.seek :topVent @08:40:05.74 26.8psi after gulp @08:40:36.34 19.3psi after gulp (stable) @08:40:36.35 Air stroke #5 of 8 @08:40:36.52 Air.in :pressurize @08:40:36.62 Air.seek :pressurized @08:40:43.17 18.8psi after pressurize @08:40:43.20 Air.in :compress @08:40:43.33 Air.seek :evac @08:40:46.39 25.8psi after compress @08:40:57.49 28.0psi after compress (stable) @08:40:57.52 Air.in :gulp @08:40:57.65 Air.seek :topVent @08:41:03.53 25.7psi after gulp @08:41:04.82 Reached 24.5 of 30.0psi after pumping 7969uL (5.00 strokes) @08:41:04.84 Air.in :default @08:41:04.97 Air.hold @08:41:05.09 Handoff.select :waste @08:41:12.35 delay 1:00 @08:42:12.38 Handoff.select :blocked @08:42:35.13 Handoff.select :waste @08:42:35.30 0.4psi at evacEnd @08:42:35.33 Air.seek :fVent Air Syringe (AirS) at fVent @08:44:22.96 -> Cartridge.sampler.deliverLysis; padProfile; Cartridge.sampler.recoverLysate @08:44:23.11 Lysis1.in :slow @08:44:23.21 Lysis1.seek 1050uL @08:44:53.26 delay 5 seconds @08:44:58.28 Handoff.select :blocked @08:44:58.53 Lysis1.seek 450uL @08:45:58.48 delay 3 seconds @08:46:01.59 Air.in mix with 1.40048 parts Lysis1 @08:46:02.36 Air.seek :evac @08:46:02.42

Lysis1.seek 300uL @08:46:17.60 Lysis1.seek :empty @08:46:47.74 Lysis buffer delivered @29.6psi @08:46:47.76 Polling Can every 2:00 @08:46:48.38 PuckHeater.configure :default #ambient 26.0C @08:46:48.48 PuckHeater.seek 79.6C @08:47:37.91 PuckHeater.hold 2:00,79.6C @08:49:37.67 Polling Can every 2:00 @08:49:38.22 PuckHeater.configure :default #ambient 26.1C @08:49:38.37 PuckHeater.hold 30 seconds,78.6C @08:50:08.56 Polling Can every 2:00 @08:50:09.11 PuckHeater.configure :default #ambient 26.1C @08:50:09.22 PuckHeater.hold 30 seconds,78C @08:50:39.35 Polling Can every 2:00 @08:50:39.90 PuckHeater.configure :default #ambient 26.1C @08:50:40.01 PuckHeater.hold 10:00,77.5C @09:00:37.66 Archive syringe pumping up to 5 strokes to reach 40psi @09:00:37.68 Archive stroke #1 of 5 @09:00:37.88 Archive.in :compress @09:00:37.98 Archive.seek :empty @09:00:38.74 28.5psi after compress @09:00:49.34 28.4psi after compress (stable) @09:00:49.37 Archive.in :gulp @09:00:49.50 Archive.seek :topVent @09:00:55.84 28.5psi after gulp @09:01:01.44 28.3psi after gulp (stable) @09:01:01.47 Archive stroke #2 of 5 @09:01:01.62 Archive.in :pressurize @09:01:01.72 Archive.seek :pressurized @09:01:14.29 27.3psi after pressurize @09:01:19.91 27.3psi after pressurize (stable) @09:01:19.94 Archive.in :compress @09:01:20.08 Archive.seek :empty @09:01:27.96 Reached 41.0 of 40.0psi after pumping 3312uL (1.91 strokes) @09:01:27.98 Archive.in :default @09:01:28.12 Archive.hold @09:01:28.26 delay 2 seconds @09:01:30.29 Archive.in :fast @09:01:30.40 Archive.seek :full @09:01:37.11 Lysis1.seek 138uL @09:01:51.04 delay 1 second @09:01:52.08 Archive.seek :full @09:01:52.19

Lysis1.seek :empty @09:02:06.28 Handoff.select :blocked @09:02:06.47 Initial air pressure @48.1psi @09:02:06.58 Lysis1.in :extract @09:02:06.72 Lysis1.seek 600uL,nil @09:02:09.25 Archive syringe pumping up to 6 strokes to reach 25psi @09:02:09.27 Archive stroke #1 of 6 @09:02:09.43 Archive.in :pressurize @09:02:09.56 Archive.seek :pressurized @09:02:22.14 14.0psi after pressurize @09:02:39.05 14.4psi after pressurize (stable) @09:02:39.08 Archive.in :compress @09:02:39.21 Archive.seek :empty @09:02:41.49 Archive.in :default @09:02:41.57 Archive.hold @09:02:41.84

Initial 600uL lysate @15.0psi @09:02:41.85 Handoff.select :blocked @09:02:42.02 Initial air pressure @15.3psi @09:02:42.15 Lysis1.seek 825uL,nil @09:02:42.37 Archive syringe pumping up to 6 strokes to reach 17psi @09:02:42.39 Archive stroke #1 of 6 @09:02:42.61 Archive.in :compress @09:02:42.75 Archive.seek :empty @09:02:51.39 Archive.in :default @09:02:51.48 Archive.hold @09:02:51.85

Recovered 825uL lysate @18.0psi @09:02:51.89 Air.in :halfSpeed @09:02:52.01 Air.seek :fullVent @09:02:54.27 Puck Top Vented, bottom @13.6psi @09:02:54.28 Handoff.select :waste @09:02:54.48 delay 5 seconds @09:02:59.57 Handoff open to waste, bottom @0.4psi @09:02:59.60 Air.in :default @09:02:59.78 Air.seek :evac 825 @09:04:04.00 -> Handoff Handoff Valve::Manifold selects waste @09:04:44.96 -> Cmd.stop @09:04:45.11 Handoff.select :close @09:04:45.30

Gate.power :heat,:OFF @09:04:45.87 Gate.power :cartridge,:OFF @09:04:46.07 PinPort.in :unlocking @09:04:46.18 PinPort.seek :clear @09:04:48.14 Carousel.in :default @09:04:48.35 Carousel.home.seek 36 @09:04:48.74 Plunge.seek :locked @09:04:49.73 Archive.in :unlocking @09:04:50.02 Archive.seek :clear @09:04:50.07 Air.in :unlocking @09:04:50.40 Air.seek :clear @09:04:50.49 Lysis1.in :unlocking @09:04:50.83 Lysis1.seek :clear @09:04:50.88 Lysis2.in :unlocking @09:04:51.09 Lysis2.seek :clear @09:04:54.87

Gate.power :motor,:OFF @09:04:54.93 Gate.power :core,:OFF @09:04:54.96 Gate.power :analytic1,:OFF @09:04:55.06 Gate.power :analytic2,:OFF @09:04:55.10 Gate.power :main,:OFF @09:04:55.15 Safely stopped and ready to power off :STOPPED @09:04:59.21 -> exit @09:04:59.24 Session Ended @09:06:34.24 -> Can Can@09:05:28, 26.1C, 42% humidity, 14.4psia, 14.888V, 0.081A, 9.399Ah, 1.21W @09:07:05.48 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @09:07:05.81 Gate.power :sampler,:OFF,limit to 3500mA @09:07:05.86 Gate.power :main,:ON,limit to 5000mA @09:07:05.90 Gate.power :analytic1,:ON,limit to 5000mA @09:07:05.93 Gate.power :analytic2,:ON,limit to 5000mA @09:07:06.05 Powering core bus @09:07:08.39 SPR Module 1, FW Rev. B, FPGA Rev. C @09:07:08.83 Archive.configure :default @09:07:08.95 Air.configure :default @09:07:09.04 Lysis1.configure :default @09:07:09.14 Lysis2.configure :default @09:07:09.27 Plunge.configure :default @09:07:09.39 SamplePump.configure :default @09:07:09.51 Toroid.configure :default @09:07:09.63 Carousel.configure :default @09:07:09.75 Pressure.configure :default @09:07:09.91 ExtraPump.configure :default @09:07:10.00 PinPort.configure :default @09:07:10.09 All elf controllers running firmware version 4.84 @09:07:10.12 Gate.power :motor,:ON,limit to 5000mA @09:07:10.41 Archive.home.jog 0 @09:07:11.21 PinPort.home.jog 0 @09:07:11.23 Carousel.home.jog 0 @09:07:11.28 Toroid.home.jog 0 @09:07:11.32 Lysis2.home.jog 0 @09:07:11.36 Lysis1.home.jog 0 @09:07:11.41 Air.home.jog 0 @09:07:11.51 Plunge.home.jog 0 @09:07:12.98 Archive.in :homing @09:07:13.40 Archive.jog 1391 @09:07:13.49 Toroid.in :homing @09:07:13.54 Lysis1.in :homing @09:07:13.62 Plunge.in :homing @09:07:13.65 Lysis2.in :homing @09:07:13.71 PinPort.in :homing @09:07:13.78 Air.in :homing @09:07:14.17 Toroid.jog 607 @09:07:14.21 Plunge.jog 1580 @09:07:14.30 Lysis1.jog 1388 @09:07:14.37 Lysis2.jog 1394 @09:07:14.46 PinPort.jog -107 @09:07:14.50 Air.jog 1371 @09:07:14.93 PinPort.setRawPosition! 0 @09:07:15.01 PinPort.seek :home @09:07:15.13 Archive.setRawPosition! 50 @09:07:15.21 Archive.seek :home @09:07:15.32 PinPort.in :default @09:07:15.51 Archive.in :default @09:07:15.86 Lysis1.setRawPosition! 50 @09:07:15.98 Lysis2.setRawPosition! 50 @09:07:16.03 Air.setRawPosition! 50 @09:07:16.06 Plunge.setRawPosition! 50 @09:07:16.09 Lysis1.seek :home @09:07:16.31 Lysis2.seek :home @09:07:16.38 Air.seek :home @09:07:16.44 Plunge.seek :home @09:07:16.75 Lysis1.in :default @09:07:16.80 Toroid.setRawPosition! 0 @09:07:16.94 Toroid.seek :home @09:07:17.08 Plunge.in :default @09:07:17.13 Lysis2.in :default @09:07:17.19 Toroid.in :default @09:07:17.24 Air.in :default @09:07:17.75 Toroid.seek :clear @09:07:19.40 Carousel.in :default @09:07:19.56 Carousel.home.seek 36 @09:07:19.89 Selecting Cartridge 35 @09:07:20.25 Carousel.seek 35 @09:07:20.83 Lysis1.in :fast @09:07:20.93 Lysis1.seek :locked @09:07:23.57 Plunge.seek :locked @09:07:23.77 Air.in :fast @09:07:23.91 Air.seek :locked @09:07:25.51 Carousel.reconfigure :locked @09:07:25.72 Powering cartridge bus @09:07:28.38 Gate.setIR 1750,5000,0,6 @09:07:28.51 Gate.setIR 0,5000,0,6 @09:07:29.00 Gate.power :cartridge,:OFF @09:07:29.67 Mounted archive Cartridge @09:07:30.08 Preparing to filter cartridge 35 @09:07:30.36 Gate.power :sampler,:ON @09:07:30.48 Priming sample loop w/150ml, bypass w/50ml @09:07:30.51 SamplePump.setPosition! 0ml @09:07:30.58 Bypass.open @09:07:32.14 Intake.open @09:07:32.94 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:07:35.53 Vacuum tare is 56% of full scale @09:07:35.74 Pressure.in :default @09:07:35.86 Pressure.hold @09:07:36.11 Waiting for external sampler to finish priming... @09:07:36.13 delayUntil "09:11:00.38" @09:11:00.43 SamplePump.in :prime @09:11:00.54 SamplePump.seek 50ml,2:53 @09:12:01.58 Pressure.stop @09:12:01.66 SamplePump.setPosition! 0ml @09:12:01.75 Exhaust.open @09:12:03.20 Bypass.close @09:12:04.79 Pressure.in :default @09:12:04.89 Pressure.hold @09:12:05.22 SamplePump.in :prime @09:12:05.33 SamplePump.seek 150ml,7:39 @09:15:07.75 Pressure.stop @09:15:07.90 Bypass.open @09:15:08.65 Intake.close @09:15:09.47 Toroid.in :hold @09:15:09.56 Toroid.seek :crack @09:15:11.13 Toroid.seek :cartridge @09:15:12.28 Toroid.in :default @09:15:12.38 Toroid.seek :clear @09:15:14.43 Pressurizing cartridge to 26psi above 13.4psia @09:15:14.45 SamplePump.setPosition! 0ml @09:15:14.54 Intake.open @09:15:15.22 Bypass.close @09:15:15.93 Exhaust.close @09:15:17.32 Pressure.in :default @09:15:17.42 Pressure.hold @09:15:17.69 SamplePump.in :charge @09:15:17.81 SamplePump.seek 2.3ml,42 seconds @09:15:28.35 Pressure.stop @09:15:28.44 Intake.close @09:15:29.20 Pressurized cartridge with 0.497ml to 26.4psi @09:15:29.22 Isolated @33.0psia (19.6psi) @09:15:29.25 Verifying cartridge holds at least 0.65..0.85 of 33.0psia @09:15:29.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:15:33.92 Cartridge pressure fell 1% to 32.7psia @09:15:34.05 Sampling 1000.0ml @09:15:34.07 SamplePump.setPosition! 0ml @09:15:34.16 Exhaust.open @09:15:34.89 Intake.open @09:15:35.73 Pressure.in :default @09:15:35.85 Pressure.hold @09:15:36.03 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @09:15:36.26 SamplePump.in :sample @09:15:36.37 SamplePump.seek 1000ml,1:23:51 @09:15:39.39 Cartridge 35 filtering 1/1000ml at 0.59ml/s with 30.9psi vacuum @09:16:39.40 Cartridge 35 filtering 32/1000ml at 0.49ml/s with 21.7psi vacuum @09:17:39.40 Cartridge 35 filtering 59/1000ml at 0.46ml/s with 26.5psi vacuum @09:18:39.40 Cartridge 35 filtering 82/1000ml at 0.39ml/s with 24.8psi vacuum @09:19:39.40 Cartridge 35 filtering 101/1000ml at 0.24ml/s with 27.0psi vacuum @09:20:39.40 Cartridge 35 filtering 116/1000ml at 0.23ml/s with 29.2psi vacuum @09:21:21.93 Flow averaged 0.36ml/s over 5:46 @09:21:22.01 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @09:21:22.61 Relieved excess vacuum in .7 seconds @09:21:22.66 SamplePump.seek 1000ml,1:13:20 @09:21:37.63 Flow averaged 0.23ml/s over 15 seconds @09:21:37.71 Waiting up to 17 seconds for vacuum to reduce from 29.5 to 18.0psi @09:21:38.31 Relieved excess vacuum in .7 seconds @09:21:38.36 SamplePump.seek 1000ml,1:13:04 @09:21:39.90 Cartridge 35 filtering 130/1000ml at 0.27ml/s with 28.5psi vacuum @09:21:48.25 Flow averaged 0.22ml/s over 10 seconds @09:21:48.33 Waiting up to 18 seconds for vacuum to reduce from 29.9 to 18.0psi @09:21:48.95 Relieved excess vacuum in .7 seconds @09:21:49.00 SamplePump.seek 1000ml,1:12:53 @09:21:56.26 Flow estimate reduced to 0.18ml/s @09:21:56.28 Intake.close @09:21:56.35 Exhaust.close @09:21:56.70 Toroid.in :hold @09:21:56.80 Toroid.seek :bypass @09:21:58.48 Toroid.in :default @09:21:58.59 Toroid.seek :clear @09:22:00.24 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:22:00.27 Pressure.stop @09:22:00.56 Sampled 133.2ml @09:22:01.08 ThreadError in detached from ben -- PROCESSING thread died holding # @09:22:01.18 Cmd::Error in detached from ben#2 -- Cannot load cartridge while in PROCESSING state @09:22:01.24 Waiting to Resume from Checkpoint "Selecting Cartridge 35" @09:22:01.41 Carousel.in :default @09:22:01.57 Carousel.home.seek 35 @09:22:01.98 Plunge.seek :locked @09:22:02.27 Air.in :unlocking @09:22:02.46 Air.seek :clear @09:22:02.58 Lysis1.in :unlocking @09:22:02.77 Lysis1.seek :clear @09:22:03.72 Gate.power :sampler,:OFF @09:22:04.30 Gate.power :motor,:OFF @09:22:04.33 Gate.power :core,:OFF @09:22:04.36 Gate.power :analytic1,:OFF @09:22:04.39 Gate.power :analytic2,:OFF @09:22:04.42 Gate.power :main,:OFF @09:22:04.46 Safely stopped and ready to power off @09:45:10.80 -> archiveEvac2AUV @09:45:10.84 Cartridge::Error in ben -- No Cartridge mounted @09:45:29.41 Session Begun @09:45:32.96 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 35 => { Type: :archiveHiBiomass_bac, State: :paused, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, lysed: 825, spr: spr23aug1107h.spr.bz2}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @09:46:26.17 -> Cartridge.log @09:46:26.23 NoMethodError in ben -- undefined method `log' for Cartridge @09:46:50.72 -> exit @09:46:50.75 Session Ended @09:47:24.28 -> MainThread.resume @09:47:24.33 Thread::CannotResume in ben -- MAIN is not suspended @09:48:45.14 -> md.stop @09:48:45.19 NoMethodError in ben -- private method `stop' called for :md:Symbol @09:48:57.20 -> Cmd.stop @09:48:57.26 ready to power off :STOPPED @09:49:20.48 -> ESP.ready! @09:49:20.54 Gate.power :sampler,:OFF,limit to 3500mA @09:49:20.56 Gate.power :main,:ON,limit to 5000mA @09:49:20.59 Gate.power :analytic1,:ON,limit to 5000mA @09:49:20.63 Gate.power :analytic2,:ON,limit to 5000mA @09:49:20.73 Powering core bus @09:49:23.08 SPR Module 1, FW Rev. B, FPGA Rev. C @09:49:23.52 Archive.configure :default @09:49:23.64 Air.configure :default @09:49:24.33 Lysis1.configure :default @09:49:24.42 Lysis2.configure :default @09:49:24.53 Plunge.configure :default @09:49:24.65 SamplePump.configure :default @09:49:24.77 Toroid.configure :default @09:49:24.88 Carousel.configure :default @09:49:25.00 Pressure.configure :default @09:49:25.12 ExtraPump.configure :default @09:49:25.21 PinPort.configure :default @09:49:25.30 All elf controllers running firmware version 4.84 @09:49:25.33 Gate.power :motor,:ON,limit to 5000mA @09:49:25.82 Archive.home.jog 0 @09:49:25.95 Air.home.jog 0 @09:49:26.63 Carousel.home.jog 0 @09:49:26.69 Plunge.home.jog 0 @09:49:26.74 Lysis2.home.jog 0 @09:49:26.78 Toroid.home.jog 0 @09:49:26.83 Lysis1.home.jog 0 @09:49:26.89 PinPort.home.jog 0 @09:49:27.63 Air.in :homing @09:49:27.85 Archive.in :homing @09:49:28.16 Air.jog 1364 @09:49:28.25 Toroid.in :homing @09:49:28.29 Lysis2.in :homing @09:49:28.33 Archive.jog 1384 @09:49:28.41 Plunge.in :homing @09:49:28.47 Lysis1.in :homing @09:49:28.51 PinPort.in :homing @09:49:28.83 Toroid.jog 581 @09:49:28.96 Lysis2.jog 1394 @09:49:29.11 Lysis1.jog 1390 @09:49:29.17 Plunge.jog 1565 @09:49:29.22 PinPort.jog -108 @09:49:29.64 PinPort.setRawPosition! 0 @09:49:29.72 PinPort.seek :home @09:49:29.89 Air.setRawPosition! 50 @09:49:29.93 PinPort.in :default @09:49:30.08 Air.seek :home @09:49:30.21 Archive.setRawPosition! 50 @09:49:30.28 Archive.seek :home @09:49:30.40 Air.in :default @09:49:30.55 Lysis2.setRawPosition! 50 @09:49:30.57 Lysis1.setRawPosition! 50 @09:49:30.72 Lysis2.seek :home @09:49:30.74 Lysis1.seek :home @09:49:30.98 Toroid.setRawPosition! 0 @09:49:31.02 Plunge.setRawPosition! 50 @09:49:31.06 Archive.in :default @09:49:31.26 Toroid.seek :home @09:49:31.30 Plunge.seek :home @09:49:31.54 Lysis2.in :default @09:49:31.57 Lysis1.in :default @09:49:31.61 Toroid.in :default @09:49:31.90 Plunge.in :default @09:49:32.26 Toroid.seek :clear @09:49:34.38 Carousel.in :default @09:49:34.54 Carousel.home.seek 35 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 35 Pressure = Absolute Pressure (Leak) Sensor @16psia PinPort = Handoff Pin Port (PP) clear @09:50:12.97 -> Cartridge.lock @09:50:13.16 Lysis1.in :fast @09:50:13.25 Lysis1.seek :locked @09:50:15.93 Plunge.seek :locked @09:50:16.13 Air.in :fast @09:50:16.22 Air.seek :locked @09:50:17.86 Carousel.reconfigure :locked @09:50:18.06 Powering cartridge bus @09:50:20.71 Gate.setIR 1750,5000,0,6 @09:50:20.84 Gate.setIR 0,5000,0,6 @09:50:20.94 Gate.power :cartridge,:OFF @09:50:21.50 Mounted archive Cartridge :archiveHiBiomass_bac @09:52:08.34 -> SC Storage Carousel (SC) at 35 @09:52:41.12 -> archiveEvac2AUV @09:52:41.26 Plunge.seek :reagent @09:52:42.11 Sample loop closed. Ready to Deliver Reagents @09:52:42.13 Archive.seek :sealed @09:52:46.13 Archive.seek :evac @09:53:00.21 delay 1:00 @09:54:00.25 Archive.seek :clear @09:54:17.78 RNAlater soak for 10:00 @10:01:36.49 Session Begun @10:01:45.68 -> clients [ESP["ben"],ESP["benji"]] @10:02:01.44 -> Can Can@10:01:15, 26.2C, 42% humidity, 14.4psia, 14.865V, 0.359A, 9.710Ah, 5.34W @10:04:17.84 Archive.seek :tVent @10:04:20.34 delay 1 second @10:04:21.37 Archive.seek :evac @10:04:36.94 delay 10 seconds @10:04:46.97 Archive.seek :tVent @10:05:02.53 delay 1 second @10:05:03.55 Archive.seek :evac @10:05:19.14 delay 10 seconds @10:05:29.18 Sample preserved and evacuated @10:07:18.53 -> Cmd.stop @10:07:18.77 Carousel.in :default @10:07:18.94 Carousel.home.seek 35 @10:07:19.34 Plunge.seek :locked @10:07:20.31 Archive.in :unlocking @10:07:20.50 Archive.seek :clear @10:07:21.13 Air.in :unlocking @10:07:21.34 Air.seek :clear @10:07:21.41 Lysis1.in :unlocking @10:07:21.67 Lysis1.seek :clear @10:07:25.77 Gate.power :motor,:OFF @10:07:25.84 Gate.power :core,:OFF @10:07:25.89 Gate.power :analytic1,:OFF @10:07:25.94 Gate.power :analytic2,:OFF @10:07:25.97 Gate.power :main,:OFF @10:07:26.02 Safely stopped and ready to power off :STOPPED @10:07:39.71 Session Ended @10:08:02.40 -> MainThread.exit # @10:08:02.51 ThreadError in ben -- killed thread @10:08:02.59 Session Ended @10:14:38.12PDT11-Aug-23

Environmental Sample Processor built 24-Jul-23 (df8af88d) @10:14:41.92 >BREAK< ESP3 Gateway boot v1.43 for core bus @10:14:41.95 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @10:14:45.53 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @10:14:46.13 Sea pressure (Abs) tare is 6% of full scale @10:14:55.29 Polling Can every 2:00 @10:14:55.36 Waiting for espclients... @10:14:55.96 Session Begun @10:15:15.36 -> ESP.ready! @10:15:15.43 Gate.power :sampler,:OFF,limit to 3500mA @10:15:15.45 Gate.power :main,:ON,limit to 5000mA @10:15:15.49 Gate.power :analytic1,:ON,limit to 5000mA @10:15:15.52 Gate.power :analytic2,:ON,limit to 5000mA @10:15:15.70 Powering core bus @10:15:17.96 SPR Module 1, FW Rev. B, FPGA Rev. C @10:15:18.49 Archive.configure :default @10:15:18.62 Air.configure :default @10:15:18.71 Lysis1.configure :default @10:15:18.79 Lysis2.configure :default @10:15:18.91 Plunge.configure :default @10:15:19.02 SamplePump.configure :default @10:15:19.13 Toroid.configure :default @10:15:19.26 Carousel.configure :default @10:15:19.37 Pressure.configure :default @10:15:19.49 ExtraPump.configure :default @10:15:19.58 PinPort.configure :default @10:15:19.66 All elf controllers running firmware version 4.84 @10:15:19.70 Gate.power :motor,:ON,limit to 5000mA @10:15:20.40 Archive.home.jog 0 @10:15:21.23 Carousel.home.jog 0 @10:15:21.26 Plunge.home.jog 0 @10:15:21.32 Lysis2.home.jog 0 @10:15:21.36 Air.home.jog 0 @10:15:21.41 PinPort.home.jog 0 @10:15:21.45 Toroid.home.jog 0 @10:15:21.48 Lysis1.home.jog 0 @10:15:22.43 Archive.in :homing @10:15:22.70 Archive.jog 1390 @10:15:22.78 Toroid.in :homing @10:15:22.82 Lysis2.in :homing @10:15:22.86 Air.in :homing @10:15:22.92 PinPort.in :homing @10:15:22.98 Plunge.in :homing @10:15:23.03 Lysis1.in :homing @10:15:23.37 Lysis2.jog 1393 @10:15:23.45 Toroid.jog 584 @10:15:23.49 PinPort.jog -107 @10:15:23.53 Air.jog 1364 @10:15:23.58 Plunge.jog 1572 @10:15:23.63 Lysis1.jog 1388 @10:15:24.01 PinPort.setRawPosition! 0 @10:15:24.09 PinPort.seek :home @10:15:24.19 PinPort.in :default @10:15:24.35 Archive.setRawPosition! 50 @10:15:24.42 Archive.seek :home @10:15:24.72 Archive.in :default @10:15:24.86 Lysis2.setRawPosition! 50 @10:15:24.93 Lysis2.seek :home @10:15:25.01 Air.setRawPosition! 50 @10:15:25.16 Plunge.setRawPosition! 50 @10:15:25.21 Lysis1.setRawPosition! 50 @10:15:25.26 Air.seek :home @10:15:25.39 Plunge.seek :home @10:15:25.48 Lysis1.seek :home @10:15:25.73 Toroid.setRawPosition! 0 @10:15:25.77 Lysis2.in :default @10:15:25.81 Air.in :default @10:15:26.01 Toroid.seek :home @10:15:26.16 Toroid.in :default @10:15:26.20 Plunge.in :default @10:15:26.24 Lysis1.in :default @10:15:26.81 Toroid.seek :clear @10:15:28.45 Carousel.in :default @10:15:28.61 Carousel.home.seek 35 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 35 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @10:18:32.48 -> start {fire :microSPR_phyto} # @10:18:33.98 Toroid.seek :clear @10:18:34.10 Carousel.in :default @10:18:34.27 Carousel.home.seek 35 @10:18:34.61 Selecting Cartridge 34 @10:18:34.96 Carousel.seek 34 @10:18:35.54 Lysis1.in :fast @10:18:35.63 Lysis1.seek :locked @10:18:38.29 Plunge.seek :locked @10:18:38.52 Air.in :fast @10:18:38.61 Air.seek :locked @10:18:40.22 Carousel.reconfigure :locked @10:18:40.45 Powering cartridge bus @10:18:43.11 Gate.setIR 1750,5000,0,6 @10:18:43.24 Gate.setIR 0,5000,0,6 @10:18:43.34 PuckHeater.configure :default @10:18:43.42 Gate.power :heat,:ON,limit to 5000mA @10:18:44.18 Mounted lysis Cartridge board#Q05066 @10:18:44.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:18:44.64 Preparing to filter cartridge 34 @10:18:44.92 Gate.power :sampler,:ON @10:18:45.03 Priming sample loop w/150ml, bypass w/50ml @10:18:45.05 SamplePump.setPosition! 0ml @10:18:45.13 Bypass.open @10:18:46.63 Intake.open @10:18:47.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:18:50.02 Vacuum tare is 56% of full scale @10:18:50.22 Pressure.in :default @10:18:50.33 Pressure.hold @10:18:50.59 Waiting for external sampler to finish priming... @10:18:50.62 delayUntil "10:22:14.94" @10:22:14.99 SamplePump.in :prime @10:22:15.09 SamplePump.seek 50ml,2:53 @10:23:16.12 Pressure.stop @10:23:16.20 SamplePump.setPosition! 0ml @10:23:16.28 Exhaust.open @10:23:17.80 Bypass.close @10:23:19.39 Pressure.in :default @10:23:19.96 Pressure.hold @10:23:20.36 SamplePump.in :prime @10:23:20.46 SamplePump.seek 150ml,7:39 @10:23:32.19 -> Can Can@10:22:54, 26.2C, 42% humidity, 14.4psia, 14.810V, 1.006A, 0.070Ah, 14.91W @10:26:22.74 Pressure.stop @10:26:22.86 Bypass.open @10:26:23.61 Intake.close @10:26:24.46 Toroid.in :hold @10:26:24.56 Toroid.seek :crack @10:26:26.13 Toroid.seek :cartridge @10:26:27.28 Toroid.in :default @10:26:27.37 Toroid.seek :clear @10:26:29.44 Pressurizing cartridge to 26psi above 13.6psia @10:26:29.46 SamplePump.setPosition! 0ml @10:26:29.55 Intake.open @10:26:30.24 Bypass.close @10:26:30.98 Exhaust.close @10:26:31.83 Pressure.in :default @10:26:31.93 Pressure.hold @10:26:32.19 SamplePump.in :charge @10:26:32.30 SamplePump.seek 2.3ml,42 seconds @10:26:45.40 Pressure.stop @10:26:45.50 Intake.close @10:26:46.26 Pressurized cartridge with 0.769ml to 26.1psi @10:26:46.27 Isolated @33.5psia (19.9psi) @10:26:46.34 Verifying cartridge holds at least 0.65..0.85 of 33.5psia @10:26:46.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:26:51.02 Cartridge pressure fell 1% to 33.3psia @10:26:51.15 Sampling 50.0ml @10:26:51.18 SamplePump.setPosition! 0ml @10:26:51.27 Exhaust.open @10:26:52.03 Intake.open @10:26:52.84 Pressure.in :default @10:26:52.94 Pressure.hold @10:26:53.11 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @10:26:53.33 SamplePump.in :sample @10:26:53.43 SamplePump.seek 50ml,4:41 @10:26:56.47 Cartridge 34 filtering 1/50ml at 0.62ml/s with 20.2psi vacuum @10:27:56.47 Cartridge 34 filtering 32/50ml at 0.46ml/s with 19.4psi vacuum @10:28:34.50 Pressure.stop @10:28:35.20 Intake.close @10:28:35.28 Exhaust.close @10:28:35.54 Toroid.in :hold @10:28:35.64 Toroid.seek :bypass @10:28:37.31 Toroid.in :default @10:28:37.40 Toroid.seek :clear @10:28:39.05 Sampled 50.0ml @10:28:40.79 SPR.command "read_sequences" @10:28:40.87 SPR.command "stats_on" @10:28:41.06 SPR.command "start 0 1" @10:28:41.75 SPR.start /var/log/esp/spr23aug1110h.spr.bz2 @10:28:42.23 Gate.power :sampler,:OFF @10:28:43.69 Handoff.select :waste @10:28:50.43 Syringe tare is 18% of full scale @10:28:50.47 PinPort.in :locking @10:28:50.58 PinPort.seek :locked @10:28:53.16 Archive.in :fast @10:28:53.22 Lysis2.in :fast @10:28:53.33 Lysis1.seek 1350uL @10:28:53.52 Archive.seek :full @10:28:53.64 Lysis2.seek 950uL @10:28:58.42 Plunge.seek :reagent @10:28:59.43 Evacuating puck with filter bubble point of 50psi @10:28:59.46 PinPort.seek :locked @10:28:59.60 Handoff.select :waste @10:29:06.23 Air syringe pumping up to 1 strokes to reach 8psi @10:29:06.25 Air stroke #1 of 1 @10:29:06.43 Air.in :pressurize @10:29:06.53 Air.seek :pressurized @10:29:12.35 0.7psi after pressurize @10:29:12.38 Air.in :compress @10:29:12.60 Air.seek :evac @10:29:15.72 1.1psi after compress @10:29:26.35 0.1psi after compress (stable) @10:29:26.37 Air.in :default @10:29:26.48 Air.hold @10:29:26.78 at -0.1psi Opened to waste @10:29:26.80 Handoff.select :blocked @10:29:26.97 Archive syringe pumping up to 8 strokes to reach 50psi @10:29:26.99 Archive stroke #1 of 8 @10:29:27.12 Archive.in :pressurize @10:29:27.21 Archive.seek :pressurized @10:29:39.75 1.8psi after pressurize @10:29:45.35 1.9psi after pressurize (stable) @10:29:45.38 Archive.in :compress @10:29:45.49 Archive.seek :empty @10:30:00.15 16.6psi after compress @10:30:11.75 19.1psi after compress (stable) @10:30:11.77 Archive.in :gulp @10:30:11.88 Archive.seek :topVent @10:30:18.18 19.8psi after gulp @10:30:23.78 19.7psi after gulp (stable) @10:30:23.79 Archive stroke #2 of 8 @10:30:23.94 Archive.in :pressurize @10:30:24.03 Archive.seek :pressurized @10:30:36.57 18.5psi after pressurize @10:30:42.17 18.4psi after pressurize (stable) @10:30:42.20 Archive.in :compress @10:30:42.36 Archive.seek :empty @10:30:57.06 31.4psi after compress @10:31:08.17 33.8psi after compress (stable) @10:31:08.20 Archive.in :gulp @10:31:08.33 Archive.seek :topVent @10:31:14.66 34.4psi after gulp @10:31:20.33 34.3psi after gulp (stable) @10:31:20.35 Archive stroke #3 of 8 @10:31:20.51 Archive.in :pressurize @10:31:20.60 Archive.seek :pressurized @10:31:33.13 34.0psi after pressurize @10:31:39.14 33.7psi after pressurize (stable) @10:31:39.28 Archive.in :compress @10:31:39.41 Archive.seek :empty @10:31:54.07 46.2psi after compress @10:31:56.41 Reached 48.1 of 50.0psi after pumping 5190uL (3.00 strokes) @10:31:56.43 Archive.in :default @10:31:56.55 Archive.hold @10:31:56.66 Handoff.select :waste @10:32:06.41 delay 1:00 @10:33:06.43 Handoff.select :blocked @10:33:29.17 Handoff.select :waste @10:33:29.33 0.6psi at evacEnd @10:33:29.35 Handoff.select :blocked @10:33:29.51 Archive.in :fast @10:33:29.61 Archive.seek :topVent @10:33:43.33 Stable @0.8psi after Handoff blocked @10:33:43.38 Archive syringe pumping up to 8 strokes to reach 31psi @10:33:43.39 Archive stroke #1 of 8 @10:33:43.53 Archive.in :pressurize @10:33:43.62 Archive.seek :pressurized @10:33:56.17 0.9psi after pressurize @10:33:56.20 Archive.in :compress @10:33:56.39 Archive.seek :empty @10:34:11.05 13.8psi after compress @10:34:21.65 15.4psi after compress (stable) @10:34:21.69 Archive.in :gulp @10:34:21.82 Archive.seek :topVent @10:34:28.14 14.8psi after gulp @10:34:33.74 14.4psi after gulp (stable) @10:34:33.76 Archive stroke #2 of 8 @10:34:33.90 Archive.in :pressurize @10:34:33.99 Archive.seek :pressurized @10:34:46.52 13.4psi after pressurize @10:34:46.55 Archive.in :compress @10:34:47.07 Archive.seek :empty @10:35:01.82 25.9psi after compress @10:35:12.42 27.5psi after compress (stable) @10:35:12.45 Archive.in :gulp @10:35:12.57 Archive.seek :topVent @10:35:18.86 26.5psi after gulp @10:35:29.01 25.0psi after gulp (stable) @10:35:29.04 Archive stroke #3 of 8 @10:35:29.20 Archive.in :pressurize @10:35:29.30 Archive.seek :pressurized @10:35:41.83 23.5psi after pressurize @10:35:41.86 Archive.in :compress @10:35:41.98 Archive.seek :empty @10:35:53.65 Reached 31.0 of 30.8psi after pumping 5124uL (2.96 strokes) @10:35:53.67 Archive.in :default @10:35:53.79 Archive.hold @10:35:53.90 Handoff.select :waste @10:36:03.13 delay 1:00 @10:37:03.15 Handoff.select :blocked @10:37:25.90 Handoff.select :waste @10:37:26.10 0.2psi at evacEnd @10:37:26.13 Air.seek :fullVent @10:37:27.74 Archive.in :fast @10:37:27.85 Archive.seek :topVent @10:37:34.77 Seawater evacuated, Pressure above/below filter released. @10:37:34.93 Lysis1.in :slow @10:37:35.02 Lysis1.seek 1050uL @10:37:38.60 Session Ended @10:38:05.07 delay 5 seconds @10:38:10.09 Handoff.select :blocked @10:38:10.32 Lysis1.seek 450uL @10:39:10.68 delay 3 seconds @10:39:13.78 Air.in mix with 1.40048 parts Lysis1 @10:39:14.47 Air.seek :evac @10:39:14.54 Lysis1.seek 300uL @10:39:29.67 Lysis1.seek :empty @10:39:59.75 Lysis buffer delivered @28.0psi @10:39:59.77 Polling Can every 2:00 @10:40:00.34 PuckHeater.configure :default #ambient 26.7C @10:40:00.42 PuckHeater.seek 79.6C @10:40:52.55 PuckHeater.hold 2:00,79.6C @10:42:52.28 Polling Can every 2:00 @10:42:52.83 PuckHeater.configure :default #ambient 26.8C @10:42:52.88 PuckHeater.hold 30 seconds,78.5C @10:43:23.00 Polling Can every 2:00 @10:43:23.55 PuckHeater.configure :default #ambient 26.8C @10:43:23.60 PuckHeater.hold 30 seconds,78C @10:43:53.64 Session Begun @10:43:53.80 Polling Can every 2:00 @10:43:54.37 PuckHeater.configure :default #ambient 26.8C @10:43:54.46 PuckHeater.hold 10:00,77.5C @10:44:04.00 -> clients [ESP["espclient"]] @10:44:19.35 -> exit @10:44:19.37 Session Ended @10:44:29.76 Session Begun @10:44:42.18 -> showlog -20 @10:53:52.72 Lysis complete. Syringe pressure @31.9psi @10:53:52.79 Archive syringe pumping up to 5 strokes to reach 40psi @10:53:52.83 Archive stroke #1 of 5 @10:53:52.97 Archive.in :pressurize @10:53:53.07 Archive.seek :pressurized @10:54:06.08 30.7psi after pressurize @10:54:11.72 30.4psi after pressurize (stable) @10:54:11.77 Archive.in :compress @10:54:11.87 Archive.seek :empty @10:54:20.46 Reached 41.2 of 40.0psi after pumping 1598uL (0.92 strokes) @10:54:20.49 Archive.in :default @10:54:20.59 Archive.hold @10:54:20.71 delay 2 seconds @10:54:22.73 Archive.in :fast @10:54:22.83 Archive.seek :full @10:54:29.58 Lysis1.seek 138uL @10:54:43.49 delay 1 second @10:54:44.53 Archive.seek :full @10:54:44.60 Lysis1.seek :empty @10:54:58.52 Handoff.select :blocked @10:54:58.67 Initial air pressure @46.7psi @10:54:58.76 Lysis1.in :extract @10:54:58.90 Lysis1.seek 600uL,nil @10:55:01.96 Archive syringe pumping up to 6 strokes to reach 25psi @10:55:01.97 Archive stroke #1 of 6 @10:55:02.11 Archive.in :pressurize @10:55:02.23 Archive.seek :pressurized @10:55:14.89 11.6psi after pressurize @10:55:20.49 11.5psi after pressurize (stable) @10:55:20.52 Archive.in :compress @10:55:20.65 Archive.seek :empty @10:55:29.47 Archive.in :default @10:55:29.56 Archive.hold @10:55:29.76 Initial 600uL lysate @17.4psi @10:55:29.78 Handoff.select :blocked @10:55:29.94 Initial air pressure @17.8psi @10:55:30.04 Lysis1.seek 825uL,nil @10:55:31.92 Archive syringe pumping up to 6 strokes to reach 17psi @10:55:31.94 Archive stroke #1 of 6 @10:55:32.11 Archive.in :compress @10:55:32.26 Archive.seek :empty @10:55:33.24 Archive.in :default @10:55:33.33 Archive.hold @10:55:33.71 Recovered 825uL lysate @14.7psi @10:55:33.74 Air.in :halfSpeed @10:55:33.85 Air.seek :fullVent @10:55:36.10 Puck Top Vented, bottom @4.9psi @10:55:36.11 Handoff.select :waste @10:55:36.29 delay 5 seconds @10:55:41.40 Handoff open to waste, bottom @0.0psi @10:55:41.42 Air.in :default @10:55:41.56 Air.seek :evac @10:55:43.02 Lysis2.in :DAmix @10:55:43.19 Lysis1.in :DAmix @10:55:43.83 Lysis2.seek 700uL @10:55:43.86 Lysis1.seek 575uL @10:56:18.98 delay 5 seconds @10:56:24.00 Handoff.select SPR @10:56:24.19 SPR.command "start 2 14" @10:56:25.51 SPR stabilized @10:56:25.71 delay 10 seconds @10:56:35.73 SPR.command "start 2 0" @10:56:38.88 Waiting up to 11:00 for SPR to enter state 1 @10:58:36.56 SPR.command "hold_on" @10:58:37.95 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @10:58:38.70 Lysis2.seek 400uL @10:58:38.75 Lysis1.seek 275uL @10:59:20.81 delay 15 seconds @10:59:35.83 SPR.command "hold_off" @10:59:38.59 Waiting up to 38:00 for SPR to enter state 7 @11:13:58.78 SPRsummary:-2.8RIU,-29.9RIU,114RIU,115RIU,high,69.5ng/L,246ng/L after 2870s @11:15:33.11 Session Ended @11:16:21.23 Session Begun @11:16:34.02 -> showlog -20 @11:18:53.71 -> Can Can@11:18:42, 26.8C, 42% humidity, 14.3psia, 14.852V, 0.529A, 0.816Ah, 7.85W @11:19:49.78 -> exit @11:19:49.81 Session Ended @11:21:10.73 Session Begun @11:21:17.21 -> showlog -20 @11:25:29.22 SPR analysis completed @11:25:36.99 Ended log spr23aug1110h.spr.bz2 @11:25:37.02 SPR.command "stats_off" @11:25:37.40 Handoff.select :close @11:25:37.58 Gate.power :heat,:OFF @11:25:37.62 Gate.power :cartridge,:OFF @11:25:37.73 PinPort.in :unlocking @11:25:37.82 PinPort.seek :clear @11:25:39.77 Carousel.in :default @11:25:39.97 Carousel.home.seek 34 @11:25:40.38 Plunge.seek :locked @11:25:41.31 Archive.in :unlocking @11:25:41.50 Archive.seek :clear @11:25:41.66 Air.in :unlocking @11:25:41.82 Air.seek :clear @11:25:41.97 Lysis1.in :unlocking @11:25:42.08 Lysis1.seek :clear @11:25:42.23 Lysis2.in :unlocking @11:25:42.37 Lysis2.seek :clear @11:25:46.22 Gate.power :motor,:OFF @11:25:46.24 Gate.power :core,:OFF @11:25:46.28 Gate.power :analytic1,:OFF @11:25:46.35 Gate.power :analytic2,:OFF @11:25:46.39 Gate.power :main,:OFF @11:25:46.41 Safely stopped and ready to power off @11:27:48.92 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @11:27:49.15 Gate.power :sampler,:OFF,limit to 3500mA @11:27:49.20 Gate.power :main,:ON,limit to 5000mA @11:27:49.25 Gate.power :analytic1,:ON,limit to 5000mA @11:27:49.28 Gate.power :analytic2,:ON,limit to 5000mA @11:27:49.39 Powering core bus @11:27:51.73 SPR Module 1, FW Rev. B, FPGA Rev. C @11:27:52.18 Archive.configure :default @11:27:52.33 Air.configure :default @11:27:52.43 Lysis1.configure :default @11:27:52.54 Lysis2.configure :default @11:27:52.67 Plunge.configure :default @11:27:52.80 SamplePump.configure :default @11:27:52.92 Toroid.configure :default @11:27:53.06 Carousel.configure :default @11:27:53.19 Pressure.configure :default @11:27:53.32 ExtraPump.configure :default @11:27:53.41 PinPort.configure :default @11:27:53.50 All elf controllers running firmware version 4.84 @11:27:53.53 Gate.power :motor,:ON,limit to 5000mA @11:27:53.93 Archive.home.jog 0 @11:27:54.10 Air.home.jog 0 @11:27:54.41 Lysis1.home.jog 0 @11:27:54.64 Lysis2.home.jog 0 @11:27:54.69 Plunge.home.jog 0 @11:27:54.84 Toroid.home.jog 0 @11:27:56.02 Carousel.home.jog 0 @11:27:56.18 PinPort.home.jog 0 @11:27:56.75 Plunge.in :homing @11:27:56.82 Lysis1.in :homing @11:27:56.87 Archive.in :homing @11:27:56.94 Toroid.in :homing @11:27:57.00 Lysis2.in :homing @11:27:57.04 Air.in :homing @11:27:57.38 Plunge.jog 1574 @11:27:57.43 Lysis1.jog 1390 @11:27:57.47 Archive.jog 1388 @11:27:57.52 Toroid.jog 596 @11:27:57.57 Lysis2.jog 1395 @11:27:57.61 Air.jog 1371 @11:27:58.05 PinPort.in :homing @11:27:58.15 PinPort.jog -107 @11:27:58.42 PinPort.setRawPosition! 0 @11:27:58.49 PinPort.seek :home @11:27:58.62 PinPort.in :default @11:27:58.87 Lysis1.setRawPosition! 50 @11:27:58.97 Lysis1.seek :home @11:27:59.13 Lysis2.setRawPosition! 50 @11:27:59.20 Air.setRawPosition! 50 @11:27:59.24 Plunge.setRawPosition! 50 @11:27:59.30 Archive.setRawPosition! 50 @11:27:59.48 Lysis2.seek :home @11:27:59.52 Air.seek :home @11:27:59.60 Plunge.seek :home @11:27:59.66 Archive.seek :home @11:28:00.06 Lysis2.in :default @11:28:00.11 Lysis1.in :default @11:28:00.17 Toroid.setRawPosition! 0 @11:28:00.41 Toroid.seek :home @11:28:00.59 Plunge.in :default @11:28:00.62 Air.in :default @11:28:00.68 Toroid.in :default @11:28:00.73 Archive.in :default @11:28:01.25 Toroid.seek :clear @11:28:02.90 Carousel.in :default @11:28:03.06 Carousel.home.seek 34 @11:28:03.37 Selecting Cartridge 33 @11:28:03.71 Carousel.seek 33 @11:28:04.33 Lysis1.in :fast @11:28:04.43 Lysis1.seek :locked @11:28:07.07 Plunge.seek :locked @11:28:07.27 Air.in :fast @11:28:07.36 Air.seek :locked @11:28:08.98 Carousel.reconfigure :locked @11:28:09.20 Powering cartridge bus @11:28:11.88 Gate.setIR 1750,5000,0,6 @11:28:12.01 Gate.setIR 0,5000,0,6 @11:28:12.12 Gate.power :cartridge,:OFF @11:28:12.70 Mounted archive Cartridge @11:28:13.10 Preparing to filter cartridge 33 @11:28:13.89 Gate.power :sampler,:ON @11:28:14.02 Priming sample loop w/150ml, bypass w/50ml @11:28:14.05 SamplePump.setPosition! 0ml @11:28:14.13 Bypass.open @11:28:15.70 Intake.open @11:28:16.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:28:19.10 Vacuum tare is 56% of full scale @11:28:19.30 Pressure.in :default @11:28:19.40 Pressure.hold @11:28:19.67 Waiting for external sampler to finish priming... @11:28:19.69 delayUntil "11:31:43.91" @11:29:42.26 Session Begun @11:29:53.07 -> upsync #<#::NoThread:0xb6cc2e00> @11:29:58.81 -> exit @11:29:58.84 Session Ended @11:31:43.97 SamplePump.in :prime @11:31:44.07 SamplePump.seek 50ml,2:53 @11:32:45.15 Pressure.stop @11:32:45.24 SamplePump.setPosition! 0ml @11:32:45.31 Exhaust.open @11:32:46.80 Bypass.close @11:32:48.44 Pressure.in :default @11:32:48.54 Pressure.hold @11:32:48.87 SamplePump.in :prime @11:32:48.97 SamplePump.seek 150ml,7:39 @11:32:52.10 SamplePump.stop @11:32:52.20 Intake.close @11:32:52.35 Exhaust.close @11:32:53.17 Pressure.stop @11:32:53.47 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.3ml @11:32:53.49 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.34ml" @11:32:56.35 Gate.power :sampler,:OFF @11:34:07.65 Session Begun @11:34:19.36 -> showlog -20 @11:41:03.08 Session Ended @11:41:43.79 -> showlog -20 @11:41:59.80 -> Power {analytic1: true, analytic2: true, cartridge: false, core: true, heat: false, main: true, motor: true, sampler: false} @11:46:08.98 -> SP Sample Pump (SP) at 2.35ml @11:54:27.02 Session Begun @11:55:54.83 -> #Ben are you there. Do you know if the pump is still running? @11:56:43.37 Session Begun @11:57:30.43 -> # my other terminal froze, pump is not currently powered @11:58:32.07 -> #I was going to try Cmd.resume, but we diceded to see what you thought first @11:59:02.24 -> #I don't think the resume command will turn the pump on. Do you know how to turn it on manually? @11:59:49.19 -> # Power.sampler :ON? @12:00:05.27 -> #yes! except the ? haha @12:00:17.99 -> #I'll run it. @12:00:21.12 -> Power.sampler :ON @12:00:21.15 Gate.power :sampler,:ON true @12:00:29.03 -> #Thanks! @12:00:30.16 -> #Its on @12:01:11.43 -> Intake.open; PB.open; SP.setPosition=0; SP.pump 30, :slow @12:01:11.46 Intake.open @12:01:12.26 Bypass.open @12:01:13.14 NoMethodError in preston -- undefined method `setPosition=' for SamplePump:Pump @12:01:22.72 -> Intake.open; PB.open; SP.position=0; SP.pump 30, :slow @12:01:22.75 SamplePump.setPosition! 0ml @12:01:22.95 SamplePump.in :slow @12:01:23.10 SamplePump.seek 30ml @12:01:37.35 -> # I have bad reception right now so it is taking a while for messages to get through Sample Pump (SP) at 30ml @12:02:45.94 -> PB.close; Exhaust.open @12:02:45.98 Bypass.close @12:02:46.77 Exhaust.open Exhaust is opened @12:03:07.94 -> SP.pump 80 @12:03:08.09 SamplePump.in :default @12:03:08.21 SamplePump.seek 110ml Sample Pump (SP) at 110ml @12:05:04.32 -> BACsampler.prime @12:05:04.49 Priming sample loop w/150ml, bypass w/50ml @12:05:04.51 SamplePump.setPosition! 0ml @12:05:04.63 Exhaust.close @12:05:05.37 Bypass.open @12:05:07.06 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:05:09.67 Vacuum tare is 56% of full scale @12:05:09.90 Pressure.in :default @12:05:10.02 Pressure.hold @12:05:10.36 SamplePump.in :prime @12:05:10.49 SamplePump.seek 50ml,2:53 @12:05:13.95 SamplePump.stop @12:05:14.07 Intake.close @12:05:14.17 Bypass.close @12:05:15.08 Pressure.stop @12:05:15.21 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.6ml @12:05:38.63 -> Intake.open; PB.open @12:05:38.66 Intake.open @12:05:39.44 Bypass.open Bypass (PB) is opened @12:05:50.98 Session Begun @12:05:56.97 -> Sampler.psi -0.153664807320411 @12:06:10.79 -> Exhaust.open @12:06:10.82 Exhaust.open Exhaust is opened @12:06:15.11 -> Sampler.calibrate @12:06:15.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:06:17.89 Vacuum tare is 56% of full scale 0.009039106(PSI/count)-329.5 @12:06:35.51 -> Power.status {analytic1: ON with current limited to 5000mA, analytic2: ON with current limited to 5000mA, cartridge: off, core: ON, heat: off with current limited to 5000mA, main: ON with current limited to 5000mA, motor: ON with current limited to 5000mA, sampler: ON with current limited to 3500mA} @12:06:47.68 -> Exhaust.close @12:06:47.71 Exhaust.close Exhaust is closed @12:07:05.99 -> SP.pump 30 @12:07:06.74 SamplePump.in :default @12:07:06.88 SamplePump.seek 32.56ml @12:07:07.43 -> loop {Sampler.psi} @12:07:17.12 UserAbort in preston2 -- User Abort!! @12:07:25.29 -> loop {puts Sampler.psi} @12:07:38.11 UserAbort in preston2 -- User Abort!! Sample Pump (SP) at 32.56ml @12:08:15.04 -> BACsampler.bypassVacuum @12:08:15.08 NoMethodError in preston -- undefined method `bypassVacuum' for BACsampler:Cartridge::Sampler @12:08:22.11 -> BACsampler.xray @12:09:15.36 -> BACsampler.prime @12:09:15.53 Priming sample loop w/150ml, bypass w/50ml @12:09:15.57 SamplePump.setPosition! 0ml @12:09:16.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:09:19.20 Vacuum tare is 56% of full scale @12:09:19.45 Pressure.in :default @12:09:19.60 Pressure.hold @12:09:20.42 SamplePump.in :prime @12:09:20.68 SamplePump.seek 50ml,2:53 @12:09:50.63 -> loop {puts Sampler.psi} @12:09:56.97 UserAbort in preston2 -- User Abort!! @12:10:21.77 Pressure.stop @12:10:21.91 SamplePump.setPosition! 0ml @12:10:22.01 Exhaust.open @12:10:23.52 Bypass.close @12:10:25.23 Pressure.in :default @12:10:25.37 Pressure.hold @12:10:25.77 SamplePump.in :prime @12:10:25.92 SamplePump.seek 150ml,7:39 @12:11:27.45 -> loop {puts Sampler.psi} @12:11:30.57 UserAbort in preston2 -- User Abort!! @12:13:28.41 Pressure.stop BACsampler @12:14:09.80 -> Intake Intake is opened @12:14:16.99 -> Exhaust Exhaust is opened @12:15:34.83 -> Thread[:FILTERING].resume @12:15:34.93 Resuming Thread after Sample Pump (SP) pressureOutOfBounds at 2.34ml at 11:32:51 # @12:15:35.03 SamplePump.seek 150ml,7:39 @12:15:35.27 Pressure.stop @12:15:35.42 Bypass.open @12:15:36.22 Intake.close @12:15:37.12 Toroid.in :hold @12:15:37.28 Toroid.seek :crack @12:15:38.91 Toroid.seek :cartridge @12:15:40.10 Toroid.in :default @12:15:40.25 Toroid.seek :clear @12:15:42.45 Pressurizing cartridge to 26psi above 13.4psia @12:15:42.49 SamplePump.setPosition! 0ml @12:15:42.67 Intake.open @12:15:43.39 Bypass.close @12:15:44.16 Exhaust.close @12:15:45.09 Pressure.in :default @12:15:45.25 Pressure.hold @12:15:45.57 SamplePump.in :charge @12:15:45.72 SamplePump.seek 2.3ml,42 seconds @12:15:54.45 Pressure.stop @12:15:54.61 Intake.close @12:15:55.43 Pressurized cartridge with 0.336ml to 26.1psi @12:15:55.45 Isolated @34.1psia (20.8psi) @12:15:55.53 Verifying cartridge holds at least 0.65..0.85 of 34.1psia @12:15:55.63 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:16:00.36 Cartridge pressure fell 3% to 33.0psia @12:16:00.55 Sampling 1000.0ml @12:16:00.58 SamplePump.setPosition! 0ml @12:16:00.74 Exhaust.open @12:16:01.53 Intake.open @12:16:03.06 Pressure.in :default @12:16:03.23 Pressure.hold @12:16:03.46 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:16:03.74 SamplePump.in :sample @12:16:03.90 SamplePump.seek 1000ml,1:23:51 @12:16:06.87 Cartridge 33 filtering 1/1000ml at 0.62ml/s with 24.1psi vacuum @12:16:30.88 -> exit @12:16:30.91 Session Ended @12:17:06.88 Cartridge 33 filtering 32/1000ml at 0.43ml/s with 27.5psi vacuum @12:17:32.87 -> exit @12:17:32.90 Session Ended @12:18:06.88 Cartridge 33 filtering 60/1000ml at 0.46ml/s with 23.1psi vacuum @12:19:06.88 Cartridge 33 filtering 84/1000ml at 0.42ml/s with 28.8psi vacuum @12:20:06.88 Cartridge 33 filtering 106/1000ml at 0.35ml/s with 29.7psi vacuum @12:21:06.87 Cartridge 33 filtering 125/1000ml at 0.30ml/s with 21.5psi vacuum @12:21:55.11 -> Can Can@12:20:29, 26.9C, 42% humidity, 14.3psia, 14.824V, 0.847A, 1.329Ah, 12.56W @12:22:06.86 Cartridge 33 filtering 142/1000ml at 0.23ml/s with 15.9psi vacuum @12:23:06.88 Cartridge 33 filtering 156/1000ml at 0.20ml/s with 27.0psi vacuum @12:23:33.03 Flow averaged 0.36ml/s over 7:30 @12:23:33.12 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @12:23:33.73 Relieved excess vacuum in .7 seconds @12:23:33.80 SamplePump.seek 1000ml,1:10:20 @12:23:49.18 Flow averaged 0.22ml/s over 16 seconds @12:23:49.28 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @12:23:49.89 Relieved excess vacuum in .7 seconds @12:23:49.95 SamplePump.seek 1000ml,1:10:03 @12:23:59.91 Flow averaged 0.22ml/s over 10 seconds @12:24:00.00 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @12:24:00.62 Relieved excess vacuum in .7 seconds @12:24:00.69 SamplePump.seek 1000ml,1:09:52 @12:24:06.87 Cartridge 33 filtering 169/1000ml at 0.28ml/s with 21.6psi vacuum @12:24:09.30 Flow estimate reduced to 0.17ml/s @12:24:09.34 Intake.close @12:24:09.41 Exhaust.close @12:24:09.77 Toroid.in :hold @12:24:09.89 Toroid.seek :bypass @12:24:11.59 Toroid.in :default @12:24:11.70 Toroid.seek :clear @12:24:13.36 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @12:24:13.41 Pressure.stop @12:24:13.70 Sampled 169.6ml @12:24:14.98 Plunge.seek :reagent @12:24:15.83 Sample loop closed. Ready to Deliver Reagents @12:24:15.86 Archive.seek :sealed @12:24:16.87 Gate.power :sampler,:OFF @12:24:19.87 Archive.seek :evac @12:24:33.95 delay 1:00 @12:25:33.99 Archive.seek :clear @12:25:51.53 RNAlater soak for 10:00 @12:35:51.56 Archive.seek :tVent @12:35:54.06 delay 1 second @12:35:55.09 Archive.seek :evac @12:36:10.65 delay 10 seconds @12:36:20.69 Archive.seek :tVent @12:36:29.90 -> exit @12:36:29.93 Session Ended @12:36:36.25 delay 1 second @12:36:37.30 Archive.seek :evac @12:36:52.85 delay 10 seconds @12:37:02.89 Sample preserved and evacuated @12:37:03.05 Selecting Cartridge 32 @12:37:03.33 Carousel.in :default @12:37:03.49 Carousel.home.seek 33 @12:37:03.88 Archive.in :unlocking @12:37:04.06 Archive.seek :clear @12:37:04.17 Air.in :unlocking @12:37:04.42 Air.seek :clear @12:37:08.79 Lysis1.in :unlocking @12:37:08.98 Lysis1.seek :clear @12:37:09.04 Plunge.in :unlocking @12:37:09.19 Plunge.seek :clear @12:37:10.63 Carousel.seek 32 @12:37:11.22 Lysis1.in :fast @12:37:11.32 Lysis1.seek :locked @12:37:13.96 Plunge.in :default @12:37:14.09 Plunge.seek :locked @12:37:14.25 Air.in :fast @12:37:14.37 Air.seek :locked @12:37:15.97 Carousel.reconfigure :locked @12:37:16.17 Powering cartridge bus @12:37:18.83 Gate.setIR 1750,5000,0,6 @12:37:18.96 Gate.setIR 0,5000,0,6 @12:37:19.06 PuckHeater.configure :default #ambient 26.8C @12:37:19.14 Gate.power :heat,:ON,limit to 5000mA /home/esp/esp2/lib/log.rb:470: [BUG] gc_sweep(): unknown data type 0x0(0xb6897850) ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] @13:41:46.25PDT11-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @13:41:47.83 >BREAK< ESP3 Gateway boot v1.43 for core bus @13:41:47.86 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @13:41:51.27 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @13:41:51.86 Sea pressure (Abs) tare is 6% of full scale @13:42:00.87 Polling Can every 2:00 @13:42:00.91 Waiting for espclients... @05:01:44.55PDT12-Aug-23 Session Begun @05:01:52.97 -> can Can@05:01:53, 22.3C, 48% humidity, 14.2psia, 14.874V, 0.087A, 2.614Ah, 1.30W @06:52:15.91 -> can Can@06:52:15, 22.8C, 47% humidity, 14.2psia, 14.875V, 0.088A, 2.762Ah, 1.31W @06:55:28.73 Session Begun @06:56:02.46 -> Can Can@06:55:10, 22.8C, 47% humidity, 14.2psia, 14.887V, 0.081A, 2.766Ah, 1.20W @07:11:25.07 -> show log -20 @07:11:25.10 -20 @07:11:25.16 NoMethodError in beadle -- undefined method `show' for CmdServer::Client:Class @07:11:35.08 -> sho log -20 @07:11:35.10 -20 @07:11:35.16 NoMethodError in beadle -- undefined method `sho' for CmdServer::Client:Class @07:11:40.14 -> showlog -20 @07:11:48.38 -> showlog -120 @07:16:48.85 Session Begun @07:17:08.12 -> exit @07:17:08.15 Session Ended @07:21:53.72 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @07:37:40.92 Session Ended @07:38:40.45 Session Begun @07:43:46.31 -> Cartridge.reserve 32 1 @07:43:50.50 -> slots {[32,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @07:55:48.53 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @07:55:49.31 Gate.power :sampler,:OFF,limit to 3500mA @07:55:49.45 Gate.power :main,:ON,limit to 5000mA @07:55:49.50 Gate.power :analytic1,:ON,limit to 5000mA @07:55:49.56 Gate.power :analytic2,:ON,limit to 5000mA @07:55:49.75 Powering core bus @07:55:52.02 SPR Module 1, FW Rev. B, FPGA Rev. C @07:55:52.53 Archive.configure :default @07:55:52.68 Air.configure :default @07:55:52.79 Lysis1.configure :default @07:55:52.91 Lysis2.configure :default @07:55:53.08 Plunge.configure :default @07:55:53.22 SamplePump.configure :default @07:55:53.35 Toroid.configure :default @07:55:53.50 Carousel.configure :default @07:55:53.65 Pressure.configure :default @07:55:53.79 ExtraPump.configure :default @07:55:53.90 PinPort.configure :default @07:55:54.01 All elf controllers running firmware version 4.84 @07:55:54.06 Gate.power :motor,:ON,limit to 5000mA @07:55:54.28 Archive.home.jog 0 @07:55:55.18 PinPort.home.jog 0 @07:55:55.23 Carousel.home.jog 0 @07:55:55.28 Plunge.home.jog 0 @07:55:55.36 Lysis1.home.jog 0 @07:55:55.41 Air.home.jog 0 @07:55:55.47 Toroid.home.jog 0 @07:55:55.53 Lysis2.home.jog 0 @07:55:56.28 Archive.in :homing @07:55:56.73 Lysis1.in :homing @07:55:56.81 Archive.jog 1389 @07:55:56.88 Toroid.in :homing @07:55:56.98 Lysis2.in :homing @07:55:57.07 Air.in :homing @07:55:57.15 Plunge.in :homing @07:55:57.22 PinPort.in :homing @07:55:57.58 Lysis1.jog 1386 @07:55:57.71 Lysis2.jog 1393 @07:55:57.74 Toroid.jog 596 @07:55:57.80 Air.jog 1372 @07:55:57.88 Plunge.jog 1566 @07:55:57.94 PinPort.jog -107 @07:55:58.45 PinPort.setRawPosition! 0 @07:55:58.56 PinPort.seek :home @07:55:58.72 Archive.setRawPosition! 50 @07:55:58.75 PinPort.in :default @07:55:58.91 Archive.seek :home @07:55:59.20 Lysis1.setRawPosition! 50 @07:55:59.32 Lysis1.seek :home @07:55:59.51 Plunge.setRawPosition! 50 @07:55:59.54 Lysis2.setRawPosition! 50 @07:55:59.59 Archive.in :default @07:55:59.65 Air.setRawPosition! 50 @07:55:59.85 Plunge.seek :home @07:56:00.02 Lysis2.seek :home @07:56:00.08 Air.seek :home @07:56:00.39 Toroid.setRawPosition! 0 @07:56:00.44 Plunge.in :default @07:56:00.56 Lysis1.in :default @07:56:00.69 Toroid.seek :home @07:56:01.00 Air.in :default @07:56:01.05 Toroid.in :default @07:56:01.11 Lysis2.in :default @07:56:01.61 Toroid.seek :clear @07:56:03.45 Carousel.in :default @07:56:03.64 Carousel.home.seek 32 @07:56:03.98 Selecting Cartridge 31 @07:56:04.36 Carousel.seek 31 @07:56:04.99 Lysis1.in :fast @07:56:05.11 Lysis1.seek :locked @07:56:07.77 Plunge.seek :locked @07:56:07.99 Air.in :fast @07:56:08.13 Air.seek :locked @07:56:09.74 Carousel.reconfigure :locked @07:56:09.96 Powering cartridge bus @07:56:12.62 Gate.setIR 1750,5000,0,6 @07:56:12.75 Gate.setIR 0,5000,0,6 @07:56:12.86 Gate.power :cartridge,:OFF @07:56:13.45 Mounted archive Cartridge @07:56:13.83 Preparing to filter cartridge 31 @07:56:14.13 Gate.power :sampler,:ON @07:56:14.27 Priming sample loop w/150ml, bypass w/50ml @07:56:14.29 SamplePump.setPosition! 0ml @07:56:14.40 Bypass.open @07:56:15.98 Intake.open @07:56:16.75 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:56:19.36 Vacuum tare is 56% of full scale @07:56:19.57 Pressure.in :default @07:56:19.68 Pressure.hold @07:56:19.94 Waiting for external sampler to finish priming... @07:56:19.96 delayUntil "07:59:44.15" @07:59:44.20 SamplePump.in :prime @07:59:44.38 SamplePump.seek 50ml,2:53 @08:00:45.47 Pressure.stop @08:00:45.57 SamplePump.setPosition! 0ml @08:00:45.66 Exhaust.open @08:00:47.12 Bypass.close @08:00:48.78 Pressure.in :default @08:00:48.88 Pressure.hold @08:00:49.29 SamplePump.in :prime @08:00:49.43 SamplePump.seek 150ml,7:39 @08:01:47.79 -> exit @08:01:47.91 Session Ended @08:03:51.84 Pressure.stop @08:03:51.96 Bypass.open @08:03:52.74 Intake.close @08:03:53.54 Toroid.in :hold @08:03:53.64 Toroid.seek :crack @08:03:55.21 Toroid.seek :cartridge @08:03:56.36 Toroid.in :default @08:03:56.45 Toroid.seek :clear @08:03:58.51 Pressurizing cartridge to 26psi above 13.6psia @08:03:58.53 SamplePump.setPosition! 0ml @08:03:58.62 Intake.open @08:03:59.31 Bypass.close @08:04:00.05 Exhaust.close @08:04:00.94 Pressure.in :default @08:04:01.07 Pressure.hold @08:04:01.35 SamplePump.in :charge @08:04:01.46 SamplePump.seek 2.3ml,42 seconds @08:04:09.67 Pressure.stop @08:04:09.77 Intake.close @08:04:10.55 Pressurized cartridge with 0.300ml to 26.1psi @08:04:10.56 Isolated @34.9psia (21.3psi) @08:04:10.59 Verifying cartridge holds at least 0.65..0.85 of 34.9psia @08:04:10.66 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:04:15.28 Cartridge pressure fell 2% to 34.4psia @08:04:15.41 Sampling 1000.0ml @08:04:15.43 SamplePump.setPosition! 0ml @08:04:15.51 Exhaust.open @08:04:16.25 Intake.open @08:04:17.14 Pressure.in :default @08:04:17.24 Pressure.hold @08:04:17.41 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:04:17.63 SamplePump.in :sample @08:04:17.74 SamplePump.seek 1000ml,1:23:51 @08:04:20.76 Cartridge 31 filtering 1/1000ml at 0.66ml/s with 24.2psi vacuum @08:05:20.76 Cartridge 31 filtering 35/1000ml at 0.52ml/s with 32.1psi vacuum @08:06:20.77 Cartridge 31 filtering 66/1000ml at 0.45ml/s with 14.0psi vacuum @08:07:20.77 Cartridge 31 filtering 94/1000ml at 0.49ml/s with 28.0psi vacuum @08:08:20.76 Cartridge 31 filtering 122/1000ml at 0.48ml/s with 28.7psi vacuum @08:09:20.76 Cartridge 31 filtering 148/1000ml at 0.49ml/s with 23.2psi vacuum @08:10:21.33 Cartridge 31 filtering 173/1000ml at 0.42ml/s with 24.2psi vacuum @08:11:20.77 Cartridge 31 filtering 197/1000ml at 0.34ml/s with 28.0psi vacuum @08:12:20.77 Cartridge 31 filtering 219/1000ml at 0.41ml/s with 18.3psi vacuum 2.6m deep (18.5psia) @08:13:20.77 Cartridge 31 filtering 241/1000ml at 0.38ml/s with 31.0psi vacuum @08:14:20.77 Cartridge 31 filtering 261/1000ml at 0.30ml/s with 24.5psi vacuum @08:15:20.77 Cartridge 31 filtering 279/1000ml at 0.36ml/s with 21.7psi vacuum @08:16:20.77 Cartridge 31 filtering 297/1000ml at 0.27ml/s with 20.6psi vacuum @08:17:20.77 Cartridge 31 filtering 313/1000ml at 0.24ml/s with 27.8psi vacuum @08:18:20.77 Cartridge 31 filtering 328/1000ml at 0.27ml/s with 14.1psi vacuum @08:19:20.77 Cartridge 31 filtering 343/1000ml at 0.22ml/s with 28.7psi vacuum @08:20:20.77 Cartridge 31 filtering 356/1000ml at 0.20ml/s with 28.8psi vacuum @08:20:45.76 Flow averaged 0.37ml/s over 16:29 @08:20:45.84 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @08:20:46.46 Relieved excess vacuum in .7 seconds @08:20:46.50 SamplePump.seek 1000ml,53:42 @08:21:18.53 Flow averaged 0.22ml/s over 33 seconds @08:21:18.59 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @08:21:19.28 Relieved excess vacuum in .8 seconds @08:21:19.32 SamplePump.seek 1000ml,53:06 @08:21:20.76 Cartridge 31 filtering 369/1000ml at 0.20ml/s with 23.4psi vacuum @08:21:37.74 Flow averaged 0.22ml/s over 19 seconds @08:21:37.82 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @08:21:38.43 Relieved excess vacuum in .7 seconds @08:21:38.47 SamplePump.seek 1000ml,52:46 @08:21:55.61 Flow averaged 0.22ml/s over 18 seconds @08:21:55.69 Waiting up to 9 seconds for vacuum to reduce from 26.6 to 18.0psi @08:21:56.30 Relieved excess vacuum in .7 seconds @08:21:56.34 SamplePump.seek 1000ml,52:28 @08:22:07.90 Flow averaged 0.21ml/s over 12 seconds @08:22:07.99 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @08:22:08.60 Relieved excess vacuum in .7 seconds @08:22:08.64 SamplePump.seek 1000ml,52:15 @08:22:17.35 Flow estimate reduced to 0.17ml/s @08:22:17.39 Intake.close @08:22:17.48 Exhaust.close @08:22:17.78 Toroid.in :hold @08:22:17.88 Toroid.seek :bypass @08:22:19.56 Toroid.in :default @08:22:19.67 Toroid.seek :clear @08:22:20.77 Cartridge 31 filtering 381/1000ml at 0.00ml/s with 0.1psi vacuum @08:22:21.32 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:22:21.35 Pressure.stop @08:22:21.63 Sampled 380.9ml @08:22:22.96 Plunge.seek :reagent @08:22:23.82 Sample loop closed. Ready to Deliver Reagents @08:22:23.85 Archive.seek :sealed @08:22:25.39 Gate.power :sampler,:OFF @08:22:27.83 Archive.seek :evac @08:22:41.89 delay 1:00 @08:23:41.93 Archive.seek :clear @08:23:59.47 RNAlater soak for 10:00 @08:33:59.50 Archive.seek :tVent @08:34:01.99 delay 1 second @08:34:03.02 Archive.seek :evac @08:34:18.57 delay 10 seconds @08:34:28.61 Archive.seek :tVent @08:34:44.21 delay 1 second @08:34:45.24 Archive.seek :evac @08:35:00.79 delay 10 seconds @08:35:10.83 Sample preserved and evacuated @08:35:11.02 Selecting Cartridge 30 @08:35:11.31 Carousel.in :default @08:35:11.48 Carousel.home.seek 31 @08:35:11.87 Archive.in :unlocking @08:35:12.07 Archive.seek :clear @08:35:12.22 Air.in :unlocking @08:35:12.37 Air.seek :clear @08:35:16.66 Lysis1.in :unlocking @08:35:16.84 Lysis1.seek :clear @08:35:16.89 Plunge.in :unlocking @08:35:17.08 Plunge.seek :clear @08:35:18.52 Carousel.seek 30 @08:35:19.12 Lysis1.in :fast @08:35:19.23 Lysis1.seek :locked @08:35:21.90 Plunge.in :default @08:35:22.03 Plunge.seek :locked @08:35:22.18 Air.in :fast @08:35:22.27 Air.seek :locked @08:35:23.87 Carousel.reconfigure :locked @08:35:24.07 Powering cartridge bus @08:35:26.73 Gate.setIR 1750,5000,0,6 @08:35:26.86 Gate.setIR 0,5000,0,6 @08:35:26.99 PuckHeater.configure :default @08:35:27.09 Gate.power :heat,:ON,limit to 5000mA @08:35:27.87 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @08:35:27.94 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:35:28.83 Preparing to filter cartridge 30 @08:35:29.14 Gate.power :sampler,:ON @08:35:29.27 Priming sample loop w/150ml, bypass w/50ml @08:35:29.29 SamplePump.setPosition! 0ml @08:35:29.38 Bypass.open @08:35:30.93 Intake.open @08:35:31.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:35:34.33 Vacuum tare is 56% of full scale @08:35:34.52 Pressure.in :default @08:35:34.61 Pressure.hold @08:35:34.88 Waiting for external sampler to finish priming... @08:35:34.90 delayUntil "08:38:59.15" @08:38:59.21 SamplePump.in :prime @08:38:59.32 SamplePump.seek 50ml,2:53 @08:40:00.35 Pressure.stop @08:40:00.51 SamplePump.setPosition! 0ml @08:40:00.60 Exhaust.open @08:40:02.07 Bypass.close @08:40:03.67 Pressure.in :default @08:40:03.77 Pressure.hold @08:40:04.11 SamplePump.in :prime @08:40:04.22 SamplePump.seek 150ml,7:39 @08:43:06.67 Pressure.stop @08:43:06.84 Bypass.open @08:43:07.61 Intake.close @08:43:08.43 Toroid.in :hold @08:43:08.52 Toroid.seek :crack @08:43:10.09 Toroid.seek :cartridge @08:43:11.23 Toroid.in :default @08:43:11.33 Toroid.seek :clear @08:43:13.39 Pressurizing cartridge to 26psi above 13.4psia @08:43:13.41 SamplePump.setPosition! 0ml @08:43:13.49 Intake.open @08:43:14.16 Bypass.close @08:43:14.91 Exhaust.close @08:43:15.81 Pressure.in :default @08:43:15.90 Pressure.hold @08:43:16.17 SamplePump.in :charge @08:43:16.30 SamplePump.seek 2.3ml,42 seconds @08:43:29.88 Pressure.stop @08:43:30.50 Intake.close @08:43:31.30 Pressurized cartridge with 0.834ml to 26.2psi @08:43:31.31 Isolated @33.9psia (20.4psi) @08:43:31.35 Verifying cartridge holds at least 0.65..0.85 of 33.9psia @08:43:31.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:43:36.02 Cartridge pressure fell 1% to 33.6psia @08:43:36.14 Sampling 50.0ml @08:43:36.16 SamplePump.setPosition! 0ml @08:43:36.26 Exhaust.open @08:43:37.01 Intake.open @08:43:37.90 Pressure.in :default @08:43:37.99 Pressure.hold @08:43:38.16 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:43:38.39 SamplePump.in :sample @08:43:38.50 SamplePump.seek 50ml,4:41 @08:43:41.51 Cartridge 30 filtering 1/50ml at 0.58ml/s with 25.0psi vacuum @08:44:41.53 Cartridge 30 filtering 35/50ml at 0.50ml/s with 27.2psi vacuum @08:45:08.98 Pressure.stop @08:45:09.22 Intake.close @08:45:09.27 Exhaust.close @08:45:09.62 Toroid.in :hold @08:45:09.72 Toroid.seek :bypass @08:45:11.38 Toroid.in :default @08:45:11.48 Toroid.seek :clear @08:45:13.13 Sampled 50.0ml @08:45:14.87 SPR.command "read_sequences" @08:45:15.13 SPR.command "stats_on" @08:45:15.31 SPR.command "start 0 1" @08:45:16.01 SPR.start /var/log/esp/spr23aug1208h.spr.bz2 @08:45:16.31 Gate.power :sampler,:OFF @08:45:17.98 Handoff.select :waste @08:45:24.72 Syringe tare is 18% of full scale @08:45:24.75 PinPort.in :locking @08:45:24.86 PinPort.seek :locked @08:45:27.43 Archive.in :fast @08:45:27.55 Lysis2.in :fast @08:45:27.64 Lysis1.seek 1350uL @08:45:27.68 Archive.seek :full @08:45:27.92 Lysis2.seek 950uL @08:45:32.68 Plunge.seek :reagent @08:45:33.69 Evacuating puck with filter bubble point of 50psi @08:45:33.72 PinPort.seek :locked @08:45:33.88 Handoff.select :waste @08:45:40.49 Air syringe pumping up to 1 strokes to reach 8psi @08:45:40.51 Air stroke #1 of 1 @08:45:40.66 Air.in :pressurize @08:45:40.75 Air.seek :pressurized @08:45:46.54 0.6psi after pressurize @08:45:46.58 Air.in :compress @08:45:46.68 Air.seek :evac @08:45:49.77 0.6psi after compress @08:46:00.37 0.0psi after compress (stable) @08:46:00.39 Air.in :default @08:46:00.50 Air.hold @08:46:00.76 at 0.0psi Opened to waste @08:46:00.78 Handoff.select :blocked @08:46:01.01 Archive syringe pumping up to 8 strokes to reach 50psi @08:46:01.04 Archive stroke #1 of 8 @08:46:01.19 Archive.in :pressurize @08:46:01.30 Archive.seek :pressurized @08:46:13.84 5.8psi after pressurize @08:46:21.44 6.8psi after pressurize (stable) @08:46:21.47 Archive.in :compress @08:46:21.58 Archive.seek :empty @08:46:36.25 23.6psi after compress @08:46:49.85 28.6psi after compress (stable) @08:46:49.88 Archive.in :gulp @08:46:49.99 Archive.seek :topVent @08:46:56.29 29.0psi after gulp @08:47:01.89 29.0psi after gulp (stable) @08:47:01.90 Archive stroke #2 of 8 @08:47:02.04 Archive.in :pressurize @08:47:02.14 Archive.seek :pressurized @08:47:14.69 28.0psi after pressurize @08:47:20.29 28.2psi after pressurize (stable) @08:47:20.32 Archive.in :compress @08:47:20.46 Archive.seek :empty @08:47:35.15 42.3psi after compress @08:47:46.75 45.6psi after compress (stable) @08:47:46.79 Archive.in :gulp @08:47:46.90 Archive.seek :topVent @08:47:53.27 45.9psi after gulp @08:47:58.99 45.9psi after gulp (stable) @08:47:59.00 Archive stroke #3 of 8 @08:47:59.15 Archive.in :pressurize @08:47:59.27 Archive.seek :pressurized @08:47:59.52 Reached 46.0 of 50.0psi after pumping 3460uL (2.00 strokes) @08:47:59.54 Archive.in :default @08:47:59.65 Archive.hold @08:47:59.97 Handoff.select :waste @08:48:11.22 delay 1:00 @08:49:11.24 Handoff.select :blocked @08:49:33.98 Handoff.select :waste @08:49:34.13 0.5psi at evacEnd @08:49:34.15 Handoff.select :blocked @08:49:34.34 Archive.in :fast @08:49:34.43 Archive.seek :topVent @08:49:41.89 Stable @0.5psi after Handoff blocked @08:49:41.92 Archive syringe pumping up to 8 strokes to reach 30psi @08:49:41.94 Archive stroke #1 of 8 @08:49:42.10 Archive.in :pressurize @08:49:42.19 Archive.seek :pressurized @08:49:54.75 1.8psi after pressurize @08:49:54.78 Archive.in :compress @08:49:54.89 Archive.seek :empty @08:50:09.58 15.2psi after compress @08:50:20.74 15.9psi after compress (stable) @08:50:20.78 Archive.in :gulp @08:50:20.91 Archive.seek :topVent @08:50:27.24 15.9psi after gulp @08:50:32.85 15.9psi after gulp (stable) @08:50:32.86 Archive stroke #2 of 8 @08:50:33.04 Archive.in :pressurize @08:50:33.15 Archive.seek :pressurized @08:50:45.71 15.8psi after pressurize @08:50:45.77 Archive.in :compress @08:50:45.88 Archive.seek :empty @08:51:00.54 30.0psi after compress @08:51:02.27 Reached 30.5 of 30.5psi after pumping 3460uL (2.00 strokes) @08:51:02.30 Archive.in :default @08:51:02.40 Archive.hold @08:51:02.51 Handoff.select :waste @08:51:09.74 delay 1:00 @08:52:09.76 Handoff.select :blocked @08:52:32.51 Handoff.select :waste @08:52:32.69 0.2psi at evacEnd @08:52:32.72 Air.seek :fullVent @08:52:34.18 Archive.in :fast @08:52:34.28 Archive.seek :topVent @08:52:41.41 Seawater evacuated, Pressure above/below filter released. @08:52:41.55 Lysis1.in :slow @08:52:41.64 Lysis1.seek 1050uL @08:53:11.70 delay 5 seconds @08:53:16.72 Handoff.select :blocked @08:53:17.01 Lysis1.seek 450uL @08:54:17.06 delay 3 seconds @08:54:20.16 Air.in mix with 1.40048 parts Lysis1 @08:54:20.88 Air.seek :evac @08:54:21.00 Lysis1.seek 300uL @08:54:36.15 Lysis1.seek :empty @08:55:06.32 Lysis buffer delivered @30.3psi @08:55:06.34 Polling Can every 2:00 @08:55:06.91 PuckHeater.configure :default #ambient 25.0C @08:55:07.03 PuckHeater.seek 79.6C @08:56:02.13 PuckHeater.hold 2:00,79.6C @08:58:02.13 Polling Can every 2:00 @08:58:02.70 PuckHeater.configure :default #ambient 25.0C @08:58:02.81 PuckHeater.hold 30 seconds,78.6C @08:58:33.03 Polling Can every 2:00 @08:58:33.59 PuckHeater.configure :default #ambient 25.1C @08:58:33.68 PuckHeater.hold 30 seconds,78.1C @08:59:03.86 Polling Can every 2:00 @08:59:04.42 PuckHeater.configure :default #ambient 25.1C @08:59:04.50 PuckHeater.hold 10:00,77.6C @08:59:44.47 Session Begun @09:09:03.93 Lysis complete. Syringe pressure @36.7psi @09:09:04.05 Archive syringe pumping up to 5 strokes to reach 40psi @09:09:04.07 Archive stroke #1 of 5 @09:09:04.30 Archive.in :pressurize @09:09:04.42 Archive.seek :pressurized @09:09:16.99 35.3psi after pressurize @09:09:22.60 35.0psi after pressurize (stable) @09:09:22.63 Archive.in :compress @09:09:22.82 Archive.seek :empty @09:09:29.43 Reached 43.0 of 40.0psi after pumping 1554uL (0.90 strokes) @09:09:29.45 Archive.in :default @09:09:29.59 Archive.hold @09:09:29.72 delay 2 seconds @09:09:31.75 Archive.in :fast @09:09:31.86 Archive.seek :full @09:09:38.47 Lysis1.seek 138uL @09:09:52.40 delay 1 second @09:09:53.44 Archive.seek :full @09:09:53.57 Lysis1.seek :empty @09:10:07.59 Handoff.select :blocked @09:10:07.75 Initial air pressure @48.9psi @09:10:07.88 Lysis1.in :extract @09:10:08.03 Lysis1.seek 600uL,nil @09:10:10.65 Archive syringe pumping up to 6 strokes to reach 25psi @09:10:10.66 Archive stroke #1 of 6 @09:10:10.84 Archive.in :pressurize @09:10:10.96 Archive.seek :pressurized @09:10:23.62 10.9psi after pressurize @09:10:41.80 Archive.in :default @09:10:41.88 Archive.hold @09:10:42.11 Initial 600uL lysate @16.5psi @09:10:42.13 Handoff.select :blocked @09:10:42.34 Initial air pressure @16.5psi @09:10:42.46 Lysis1.seek 825uL,nil @09:10:46.30 Syringe::LysatePlungerStuck in PROCESSING -- at < 709uL @09:10:46.36 Lysis1.in :default @09:10:46.50 Lysis1.seek 674uL @09:10:48.44 Lysis1.in :extract @09:10:48.57 Lysis1.seek 825uL,nil @09:10:51.00 Recovered 825uL lysate @17.5psi @09:10:51.03 Air.in :halfSpeed @09:10:51.17 Air.seek :fullVent @09:10:53.45 Puck Top Vented, bottom @12.8psi @09:10:53.47 Handoff.select :waste @09:10:53.69 delay 5 seconds @09:10:58.79 Handoff open to waste, bottom @0.3psi @09:10:58.82 Air.in :default @09:10:58.96 Air.seek :evac @09:11:00.44 Lysis2.in :DAmix @09:11:00.63 Lysis1.in :DAmix @09:11:01.38 Lysis2.seek 700uL @09:11:01.43 Lysis1.seek 575uL @09:11:36.63 delay 5 seconds @09:11:41.65 Handoff.select SPR @09:11:41.84 SPR.command "start 2 14" @09:11:43.20 SPR stabilized @09:11:43.43 delay 10 seconds @09:11:53.45 SPR.command "start 2 0" @09:11:57.06 Waiting up to 11:00 for SPR to enter state 1 @09:13:55.16 SPR.command "hold_on" @09:13:57.06 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:13:57.75 Lysis2.seek 400uL @09:13:57.81 Lysis1.seek 275uL @09:14:39.94 delay 15 seconds @09:14:54.97 SPR.command "hold_off" @09:14:58.17 Waiting up to 38:00 for SPR to enter state 7 @09:29:17.39 SPRsummary:49.2RIU,32.7RIU,146RIU,184RIU,high,129ng/L,197ng/L after 2790s @09:40:47.83 SPR analysis completed @09:40:54.87 Ended log spr23aug1208h.spr.bz2 @09:40:54.90 SPR.command "stats_off" @09:40:55.30 Handoff.select :close @09:40:55.49 Gate.power :heat,:OFF @09:40:55.52 Gate.power :cartridge,:OFF @09:40:55.67 PinPort.in :unlocking @09:40:55.78 PinPort.seek :clear @09:40:57.75 Carousel.in :default @09:40:57.92 Carousel.home.seek 30 @09:40:58.32 Plunge.seek :locked @09:40:59.27 Archive.in :unlocking @09:40:59.47 Archive.seek :clear @09:40:59.59 Air.in :unlocking @09:40:59.80 Air.seek :clear @09:40:59.88 Lysis1.in :unlocking @09:41:00.16 Lysis1.seek :clear @09:41:00.23 Lysis2.in :unlocking @09:41:00.42 Lysis2.seek :clear @09:41:04.34 Gate.power :motor,:OFF @09:41:04.37 Gate.power :core,:OFF @09:41:04.41 Gate.power :analytic1,:OFF @09:41:04.46 Gate.power :analytic2,:OFF @09:41:04.51 Gate.power :main,:OFF @09:41:04.54 Safely stopped and ready to power off @09:42:11.57 Session Ended @09:43:00.28 Session Begun @10:01:05.65 Session Begun @10:02:52.80 -> ESP.ready!; SPR.start; delay 2; SPR.command “start 3 0”; delay ’15:00’; SPR.command “start 2 14”; SPR.stop; delay 10; Cmd.stop @10:02:52.87 SyntaxError in preston -- compile error preston:1: Invalid char `\342' in expression preston:1: Invalid char `\200' in expression preston:1: Invalid char `\234' in expression preston:1: syntax error, unexpected tINTEGER, expecting $end ...ay 2; SPR.command “start 3 0”; delay ’15:00’; SPR.co... ^ @10:03:15.43 -> ESP.ready! @10:03:15.51 Gate.power :sampler,:OFF,limit to 3500mA @10:03:15.59 Gate.power :main,:ON,limit to 5000mA @10:03:15.66 Gate.power :analytic1,:ON,limit to 5000mA @10:03:15.71 Gate.power :analytic2,:ON,limit to 5000mA @10:03:15.87 Powering core bus @10:03:18.20 SPR Module 1, FW Rev. B, FPGA Rev. C @10:03:18.66 Archive.configure :default @10:03:18.82 Air.configure :default @10:03:18.95 Lysis1.configure :default @10:03:19.10 Lysis2.configure :default @10:03:19.26 Plunge.configure :default @10:03:19.43 SamplePump.configure :default @10:03:19.58 Toroid.configure :default @10:03:19.79 Carousel.configure :default @10:03:19.93 Pressure.configure :default @10:03:20.09 ExtraPump.configure :default @10:03:20.22 PinPort.configure :default @10:03:20.34 All elf controllers running firmware version 4.84 @10:03:20.42 Gate.power :motor,:ON,limit to 5000mA @10:03:20.92 Archive.home.jog 0 @10:03:21.56 Carousel.home.jog 0 @10:03:21.63 Toroid.home.jog 0 @10:03:21.73 Plunge.home.jog 0 @10:03:21.79 Lysis2.home.jog 0 @10:03:21.85 Lysis1.home.jog 0 @10:03:21.89 Air.home.jog 0 @10:03:22.03 PinPort.home.jog 0 @10:03:22.99 Archive.in :homing @10:03:23.37 Archive.jog 1389 @10:03:23.71 Toroid.in :homing @10:03:23.77 Plunge.in :homing @10:03:23.85 Lysis1.in :homing @10:03:23.93 PinPort.in :homing @10:03:24.01 Air.in :homing @10:03:24.07 Lysis2.in :homing @10:03:24.46 Toroid.jog 589 @10:03:24.49 Plunge.jog 1577 @10:03:24.57 Lysis1.jog 1386 @10:03:24.71 PinPort.jog -107 @10:03:24.90 Air.jog 1371 @10:03:25.09 Lysis2.jog 1395 @10:03:25.46 Archive.setRawPosition! 50 @10:03:25.58 Archive.seek :home @10:03:25.76 PinPort.setRawPosition! 0 @10:03:25.86 PinPort.seek :home @10:03:26.04 Archive.in :default @10:03:26.08 PinPort.in :default @10:03:26.36 Plunge.setRawPosition! 50 @10:03:26.40 Lysis1.setRawPosition! 50 @10:03:26.60 Plunge.seek :home @10:03:26.68 Lysis1.seek :home @10:03:26.93 Toroid.setRawPosition! 0 @10:03:27.02 Lysis2.setRawPosition! 50 @10:03:27.10 Air.setRawPosition! 50 @10:03:27.81 Toroid.seek :home @10:03:27.85 Lysis2.seek :home @10:03:28.03 Air.seek :home @10:03:28.34 Plunge.in :default @10:03:28.37 Lysis1.in :default @10:03:28.51 Toroid.in :default @10:03:28.83 Air.in :default @10:03:28.86 Lysis2.in :default @10:03:29.37 Toroid.seek :clear @10:03:31.05 Carousel.in :default @10:03:31.26 Carousel.home.seek 30 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 30 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @10:03:39.68 -> SPR.start @10:03:40.91 SPR.command "read_sequences" @10:03:41.66 SPR.command "stats_on" # @10:03:42.58 SPR.start /var/log/esp/spr23aug1210h.spr.bz2 @10:03:59.36 -> SPR.command "start 3 0" @10:03:59.39 SPR.command "start 3 0" @10:05:07.14 -> delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @10:05:07.18 delay 15:00 @10:05:45.95 -> can Can@10:05:46, 25.2C, 46% humidity, 14.4psia, 14.843V, 0.458A, 4.164Ah, 6.80W @10:19:07.02 Session Ended @10:20:07.27 SPR.command "start 2 14" @10:20:14.15 Ended log spr23aug1210h.spr.bz2 @10:20:14.17 SPR.command "stats_off" @10:20:14.79 delay 10 seconds @10:20:25.03 Carousel.in :default @10:20:25.22 Carousel.home.seek 30 @10:20:25.62 Lysis1.in :fast @10:20:25.73 Lysis1.seek :locked @10:20:28.30 Plunge.seek :locked @10:20:29.69 Lysis1.in :unlocking @10:20:29.92 Lysis1.seek :clear @10:20:31.47 Gate.power :motor,:OFF @10:20:31.50 Gate.power :core,:OFF @10:20:31.56 Gate.power :analytic1,:OFF @10:20:31.61 Gate.power :analytic2,:OFF @10:20:31.65 Gate.power :main,:OFF @10:20:31.70 Safely stopped and ready to power off :STOPPED @10:22:16.24 -> exit @10:22:16.27 Session Ended @10:50:22.11 Session Ended @10:50:54.87 Session Begun @10:54:02.64 -> slot {[32,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1208h.spr.bz2}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @10:56:24.71 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @10:56:24.98 Gate.power :sampler,:OFF,limit to 3500mA @10:56:25.05 Gate.power :main,:ON,limit to 5000mA @10:56:25.08 Gate.power :analytic1,:ON,limit to 5000mA @10:56:25.12 Gate.power :analytic2,:ON,limit to 5000mA @10:56:25.22 Powering core bus @10:56:27.58 SPR Module 1, FW Rev. B, FPGA Rev. C @10:56:28.00 Archive.configure :default @10:56:28.63 Air.configure :default @10:56:28.73 Lysis1.configure :default @10:56:28.82 Lysis2.configure :default @10:56:28.98 Plunge.configure :default @10:56:29.10 SamplePump.configure :default @10:56:29.22 Toroid.configure :default @10:56:29.33 Carousel.configure :default @10:56:29.46 Pressure.configure :default @10:56:29.57 ExtraPump.configure :default @10:56:29.68 PinPort.configure :default @10:56:29.78 All elf controllers running firmware version 4.84 @10:56:29.81 Gate.power :motor,:ON,limit to 5000mA @10:56:30.25 Archive.home.jog 0 @10:56:30.92 PinPort.home.jog 0 @10:56:31.00 Toroid.home.jog 0 @10:56:31.08 Plunge.home.jog 0 @10:56:31.13 Lysis1.home.jog 0 @10:56:31.18 Air.home.jog 0 @10:56:31.24 Lysis2.home.jog 0 @10:56:31.30 Carousel.home.jog 0 @10:56:32.24 Archive.in :homing @10:56:32.57 Plunge.in :homing @10:56:32.69 Archive.jog 1389 @10:56:32.74 Toroid.in :homing @10:56:32.79 Lysis1.in :homing @10:56:32.84 Air.in :homing @10:56:32.92 PinPort.in :homing @10:56:33.01 Lysis2.in :homing @10:56:33.36 Plunge.jog 1569 @10:56:33.42 Toroid.jog 588 @10:56:33.47 Lysis1.jog 1387 @10:56:33.52 Air.jog 1367 @10:56:33.58 Lysis2.jog 1393 @10:56:33.68 PinPort.jog -107 @10:56:34.17 PinPort.setRawPosition! 0 @10:56:34.25 PinPort.seek :home @10:56:34.37 Archive.setRawPosition! 50 @10:56:34.46 Archive.seek :home @10:56:34.58 PinPort.in :default @10:56:34.77 Archive.in :default @10:56:34.91 Plunge.setRawPosition! 50 @10:56:35.05 Plunge.seek :home @10:56:35.18 Lysis1.setRawPosition! 50 @10:56:35.23 Air.setRawPosition! 50 @10:56:35.28 Lysis2.setRawPosition! 50 @10:56:35.43 Lysis1.seek :home @10:56:35.50 Air.seek :home @10:56:35.57 Lysis2.seek :home @10:56:35.88 Toroid.setRawPosition! 0 @10:56:35.91 Plunge.in :default @10:56:36.08 Toroid.seek :home @10:56:36.23 Lysis2.in :default @10:56:36.27 Lysis1.in :default @10:56:36.34 Toroid.in :default @10:56:36.41 Air.in :default @10:56:37.42 Toroid.seek :clear @10:56:39.07 Carousel.in :default @10:56:39.30 Carousel.home.seek 30 @10:56:39.61 Selecting Cartridge 29 @10:56:39.97 Carousel.seek 29 @10:56:40.54 Lysis1.in :fast @10:56:40.64 Lysis1.seek :locked @10:56:43.30 Plunge.seek :locked @10:56:43.50 Air.in :fast @10:56:43.59 Air.seek :locked @10:56:45.20 Carousel.reconfigure :locked @10:56:45.41 Powering cartridge bus @10:56:48.07 Gate.setIR 1750,5000,0,6 @10:56:48.20 Gate.setIR 0,5000,0,6 @10:56:48.30 Gate.power :cartridge,:OFF @10:56:48.87 Mounted archive Cartridge @10:56:49.28 Preparing to filter cartridge 29 @10:56:49.56 Gate.power :sampler,:ON @10:56:49.69 Priming sample loop w/150ml, bypass w/50ml @10:56:49.71 SamplePump.setPosition! 0ml @10:56:49.80 Bypass.open @10:56:51.34 Intake.open @10:56:52.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:56:54.70 Vacuum tare is 56% of full scale @10:56:54.92 Pressure.in :default @10:56:55.06 Pressure.hold @10:56:55.32 Waiting for external sampler to finish priming... @10:56:55.34 delayUntil "11:00:19.58" @11:00:19.63 SamplePump.in :prime @11:00:19.74 SamplePump.seek 50ml,2:53 @11:01:20.78 Pressure.stop @11:01:20.86 SamplePump.setPosition! 0ml @11:01:20.97 Exhaust.open @11:01:22.42 Bypass.close @11:01:24.53 Pressure.in :default @11:01:24.63 Pressure.hold @11:01:25.00 SamplePump.in :prime @11:01:25.10 SamplePump.seek 150ml,7:39 @11:04:27.46 Pressure.stop @11:04:27.57 Bypass.open @11:04:28.37 Intake.close @11:04:29.16 Toroid.in :hold @11:04:29.26 Toroid.seek :crack @11:04:30.83 Toroid.seek :cartridge @11:04:31.97 Toroid.in :default @11:04:32.08 Toroid.seek :clear @11:04:34.12 Pressurizing cartridge to 26psi above 13.5psia @11:04:34.15 SamplePump.setPosition! 0ml @11:04:34.25 Intake.open @11:04:34.92 Bypass.close @11:04:35.67 Exhaust.close @11:04:36.54 Pressure.in :default @11:04:36.64 Pressure.hold @11:04:36.91 SamplePump.in :charge @11:04:37.05 SamplePump.seek 2.3ml,42 seconds @11:04:45.47 Pressure.stop @11:04:45.56 Intake.close @11:04:46.33 Pressurized cartridge with 0.315ml to 26.2psi @11:04:46.34 Isolated @34.7psia (21.2psi) @11:04:46.36 Verifying cartridge holds at least 0.65..0.85 of 34.7psia @11:04:46.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:04:51.04 Cartridge pressure fell 3% to 33.8psia @11:04:51.18 Sampling 1000.0ml @11:04:51.20 SamplePump.setPosition! 0ml @11:04:51.29 Exhaust.open @11:04:52.05 Intake.open @11:04:52.90 Pressure.in :default @11:04:53.03 Pressure.hold @11:04:53.20 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:04:53.43 SamplePump.in :sample @11:04:54.05 SamplePump.seek 1000ml,1:23:51 @11:04:56.55 Cartridge 29 filtering 1/1000ml at 0.62ml/s with 23.9psi vacuum @11:05:56.56 Cartridge 29 filtering 34/1000ml at 0.52ml/s with 21.9psi vacuum @11:06:56.57 Cartridge 29 filtering 63/1000ml at 0.51ml/s with 16.4psi vacuum @11:07:56.56 Cartridge 29 filtering 91/1000ml at 0.43ml/s with 15.1psi vacuum @11:08:56.56 Cartridge 29 filtering 118/1000ml at 0.43ml/s with 15.8psi vacuum @11:09:56.56 Cartridge 29 filtering 143/1000ml at 0.39ml/s with 13.8psi vacuum @11:10:56.56 Cartridge 29 filtering 167/1000ml at 0.40ml/s with 28.1psi vacuum @11:11:56.56 Cartridge 29 filtering 189/1000ml at 0.34ml/s with 13.4psi vacuum @11:12:56.56 Cartridge 29 filtering 211/1000ml at 0.29ml/s with 20.6psi vacuum @11:13:56.56 Cartridge 29 filtering 231/1000ml at 0.37ml/s with 16.8psi vacuum @11:14:56.56 Cartridge 29 filtering 250/1000ml at 0.36ml/s with 25.1psi vacuum @11:15:56.56 Cartridge 29 filtering 268/1000ml at 0.24ml/s with 24.9psi vacuum @11:16:56.56 Cartridge 29 filtering 285/1000ml at 0.23ml/s with 25.9psi vacuum @11:17:56.56 Cartridge 29 filtering 301/1000ml at 0.26ml/s with 14.3psi vacuum @11:18:56.56 Cartridge 29 filtering 315/1000ml at 0.27ml/s with 29.8psi vacuum @11:19:56.56 Cartridge 29 filtering 329/1000ml at 0.25ml/s with 24.0psi vacuum @11:20:56.57 Cartridge 29 filtering 342/1000ml at 0.23ml/s with 15.6psi vacuum @11:21:18.63 Flow averaged 0.35ml/s over 16:26 @11:21:18.70 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @11:21:19.30 Relieved excess vacuum in .7 seconds @11:21:19.35 SamplePump.seek 1000ml,54:54 @11:21:56.55 Cartridge 29 filtering 355/1000ml at 0.20ml/s with 28.7psi vacuum @11:21:58.59 Flow averaged 0.22ml/s over 40 seconds @11:21:58.66 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @11:21:59.27 Relieved excess vacuum in .7 seconds @11:21:59.31 SamplePump.seek 1000ml,54:11 @11:22:19.09 Flow averaged 0.22ml/s over 20 seconds @11:22:19.17 Waiting up to 18 seconds for vacuum to reduce from 30.1 to 18.0psi @11:22:19.77 Relieved excess vacuum in .7 seconds @11:22:19.83 SamplePump.seek 1000ml,53:50 @11:22:30.03 Flow averaged 0.21ml/s over 11 seconds @11:22:30.10 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @11:22:30.71 Relieved excess vacuum in .7 seconds @11:22:30.75 SamplePump.seek 1000ml,53:39 @11:22:45.29 Flow averaged 0.21ml/s over 15 seconds @11:22:45.36 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @11:22:46.57 Relieved excess vacuum in 1.3 seconds @11:22:46.63 SamplePump.seek 1000ml,53:24 @11:22:56.56 Cartridge 29 filtering 368/1000ml at 0.20ml/s with 28.6psi vacuum @11:22:59.53 Flow averaged 0.21ml/s over 13 seconds @11:22:59.61 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @11:23:00.22 Relieved excess vacuum in .7 seconds @11:23:00.26 SamplePump.seek 1000ml,53:10 @11:23:07.55 Flow estimate reduced to 0.17ml/s @11:23:07.58 Intake.close @11:23:07.66 Exhaust.close @11:23:08.00 Toroid.in :hold @11:23:08.10 Toroid.seek :bypass @11:23:09.78 Toroid.in :default @11:23:09.89 Toroid.seek :clear @11:23:11.54 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:23:11.57 Pressure.stop @11:23:11.86 Sampled 369.8ml @11:23:12.56 Plunge.seek :reagent @11:23:13.39 Sample loop closed. Ready to Deliver Reagents @11:23:13.42 Archive.seek :sealed @11:23:15.02 Gate.power :sampler,:OFF @11:23:17.42 Archive.seek :evac @11:23:31.49 delay 1:00 @11:24:31.53 Archive.seek :clear @11:24:49.06 RNAlater soak for 10:00 @11:34:49.09 Archive.seek :tVent @11:34:51.57 delay 1 second @11:34:52.61 Archive.seek :evac @11:35:08.16 delay 10 seconds @11:35:18.19 Archive.seek :tVent @11:35:33.75 delay 1 second @11:35:34.78 Archive.seek :evac @11:35:50.33 delay 10 seconds @11:36:00.38 Sample preserved and evacuated @11:36:00.54 Selecting Cartridge 28 @11:36:00.82 Carousel.in :default @11:36:01.02 Carousel.home.seek 29 @11:36:01.42 Archive.in :unlocking @11:36:01.60 Archive.seek :clear @11:36:01.66 Air.in :unlocking @11:36:02.40 Air.seek :clear @11:36:06.23 Lysis1.in :unlocking @11:36:06.44 Plunge.in :unlocking @11:36:06.46 Lysis1.seek :clear @11:36:06.62 Plunge.seek :clear @11:36:08.09 Carousel.seek 28 @11:36:08.67 Lysis1.in :fast @11:36:08.77 Lysis1.seek :locked @11:36:11.43 Plunge.in :default @11:36:11.57 Plunge.seek :locked @11:36:11.73 Air.in :fast @11:36:11.83 Air.seek :locked @11:36:13.44 Carousel.reconfigure :locked @11:36:13.64 Powering cartridge bus @11:36:16.31 Gate.setIR 1750,5000,0,6 @11:36:16.44 Gate.setIR 0,5000,0,6 @11:36:16.53 PuckHeater.configure :default #ambient 25.1C @11:36:16.62 Gate.power :heat,:ON,limit to 5000mA @11:36:17.32 Mounted lysis Cartridge board#Q05086 @11:36:17.38 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:36:17.75 Preparing to filter cartridge 28 @11:36:18.03 Gate.power :sampler,:ON @11:36:18.16 Priming sample loop w/150ml, bypass w/50ml @11:36:18.19 SamplePump.setPosition! 0ml @11:36:18.28 Bypass.open @11:36:19.84 Intake.open @11:36:20.56 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:36:23.15 Vacuum tare is 56% of full scale @11:36:23.34 Pressure.in :default @11:36:23.43 Pressure.hold @11:36:23.70 Waiting for external sampler to finish priming... @11:36:23.72 delayUntil "11:39:48.05" @11:39:48.10 SamplePump.in :prime @11:39:48.21 SamplePump.seek 50ml,2:53 @11:40:49.24 Pressure.stop @11:40:49.32 SamplePump.setPosition! 0ml @11:40:49.40 Exhaust.open @11:40:51.41 Bypass.close @11:40:53.09 Pressure.in :default @11:40:53.18 Pressure.hold @11:40:53.54 SamplePump.in :prime @11:40:53.65 SamplePump.seek 150ml,7:39 @11:43:56.04 Pressure.stop @11:43:56.20 Bypass.open @11:43:57.00 Intake.close @11:43:57.81 Toroid.in :hold @11:43:57.91 Toroid.seek :crack @11:43:59.48 Toroid.seek :cartridge @11:44:00.63 Toroid.in :default @11:44:00.72 Toroid.seek :clear @11:44:02.77 Pressurizing cartridge to 26psi above 13.5psia @11:44:02.79 SamplePump.setPosition! 0ml @11:44:02.89 Intake.open @11:44:03.62 Bypass.close @11:44:04.35 Exhaust.close @11:44:05.22 Pressure.in :default @11:44:05.32 Pressure.hold @11:44:05.58 SamplePump.in :charge @11:44:05.69 SamplePump.seek 2.3ml,42 seconds @11:44:18.97 Pressure.stop @11:44:19.08 Intake.close @11:44:19.87 Pressurized cartridge with 0.792ml to 26.2psi @11:44:19.89 Isolated @32.7psia (19.2psi) @11:44:19.95 Verifying cartridge holds at least 0.65..0.85 of 32.7psia @11:44:20.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:44:24.69 Sampling 50.0ml @11:44:24.71 SamplePump.setPosition! 0ml @11:44:24.80 Exhaust.open @11:44:25.54 Intake.open @11:44:26.42 Pressure.in :default @11:44:26.51 Pressure.hold @11:44:26.69 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:44:27.48 SamplePump.in :sample @11:44:27.59 SamplePump.seek 50ml,4:41 @11:44:30.61 Cartridge 28 filtering 1/50ml at 0.63ml/s with 25.3psi vacuum @11:45:30.62 Cartridge 28 filtering 35/50ml at 0.56ml/s with 24.5psi vacuum @11:45:57.65 Pressure.stop @11:45:57.88 Intake.close @11:45:58.06 Exhaust.close @11:45:58.27 Toroid.in :hold @11:45:58.37 Toroid.seek :bypass @11:46:00.03 Toroid.in :default @11:46:00.13 Toroid.seek :clear @11:46:01.78 Sampled 50.0ml @11:46:03.51 SPR.command "read_sequences" @11:46:03.97 SPR.command "stats_on" @11:46:04.16 SPR.command "start 0 1" @11:46:04.86 SPR.start /var/log/esp/spr23aug1211h.spr.bz2 @11:46:04.96 Gate.power :sampler,:OFF @11:46:06.83 Handoff.select :waste @11:46:13.58 Syringe tare is 18% of full scale @11:46:13.62 PinPort.in :locking @11:46:13.73 PinPort.seek :locked @11:46:16.30 Archive.in :fast @11:46:16.43 Lysis2.in :fast @11:46:16.51 Lysis1.seek 1350uL @11:46:16.54 Archive.seek :full @11:46:16.80 Lysis2.seek 950uL @11:46:21.56 Plunge.seek :reagent @11:46:23.12 Evacuating puck with filter bubble point of 50psi @11:46:23.15 PinPort.seek :locked @11:46:23.31 Handoff.select :waste @11:46:29.97 Air syringe pumping up to 1 strokes to reach 8psi @11:46:30.00 Air stroke #1 of 1 @11:46:30.16 Air.in :pressurize @11:46:30.26 Air.seek :pressurized @11:46:36.05 0.4psi after pressurize @11:46:36.08 Air.in :compress @11:46:36.19 Air.seek :evac @11:46:39.24 0.7psi after compress @11:46:49.87 0.0psi after compress (stable) @11:46:49.90 Air.in :default @11:46:50.01 Air.hold @11:46:50.28 at 0.0psi Opened to waste @11:46:50.30 Handoff.select :blocked @11:46:50.47 Archive syringe pumping up to 8 strokes to reach 50psi @11:46:50.48 Archive stroke #1 of 8 @11:46:51.17 Archive.in :pressurize @11:46:51.28 Archive.seek :pressurized @11:47:03.83 6.2psi after pressurize @11:47:09.43 6.3psi after pressurize (stable) @11:47:09.46 Archive.in :compress @11:47:09.57 Archive.seek :empty @11:47:24.24 22.0psi after compress @11:47:37.34 24.9psi after compress (stable) @11:47:37.37 Archive.in :gulp @11:47:37.49 Archive.seek :topVent @11:47:43.82 25.7psi after gulp @11:47:49.45 25.6psi after gulp (stable) @11:47:49.49 Archive stroke #2 of 8 @11:47:49.64 Archive.in :pressurize @11:47:49.74 Archive.seek :pressurized @11:48:02.29 24.8psi after pressurize @11:48:07.89 24.9psi after pressurize (stable) @11:48:07.92 Archive.in :compress @11:48:08.05 Archive.seek :empty @11:48:22.72 38.4psi after compress @11:48:34.83 42.1psi after compress (stable) @11:48:34.88 Archive.in :gulp @11:48:35.03 Archive.seek :topVent @11:48:41.33 43.2psi after gulp @11:48:46.93 43.1psi after gulp (stable) @11:48:46.96 Archive stroke #3 of 8 @11:48:47.15 Archive.in :pressurize @11:48:47.27 Archive.seek :pressurized @11:48:59.82 42.1psi after pressurize @11:49:05.43 42.2psi after pressurize (stable) @11:49:05.46 Archive.in :compress @11:49:05.57 Archive.seek :empty @11:49:17.56 Reached 50.2 of 50.0psi after pumping 5132uL (2.97 strokes) @11:49:17.58 Archive.in :default @11:49:17.69 Archive.hold @11:49:17.80 Handoff.select :waste @11:49:28.53 delay 1:00 @11:50:28.55 Handoff.select :blocked @11:50:51.33 Handoff.select :waste @11:50:51.48 0.4psi at evacEnd @11:50:51.50 Handoff.select :blocked @11:50:51.67 Archive.in :fast @11:50:51.76 Archive.seek :topVent @11:51:05.30 Stable @0.4psi after Handoff blocked @11:51:05.33 Archive syringe pumping up to 8 strokes to reach 30psi @11:51:05.36 Archive stroke #1 of 8 @11:51:05.52 Archive.in :pressurize @11:51:05.61 Archive.seek :pressurized @11:51:18.17 0.5psi after pressurize @11:51:18.20 Archive.in :compress @11:51:18.31 Archive.seek :empty @11:51:33.02 14.4psi after compress @11:51:43.63 15.3psi after compress (stable) @11:51:43.66 Archive.in :gulp @11:51:43.77 Archive.seek :topVent @11:51:50.07 15.4psi after gulp @11:51:59.23 15.4psi after gulp (stable) @11:51:59.24 Archive stroke #2 of 8 @11:51:59.40 Archive.in :pressurize @11:51:59.49 Archive.seek :pressurized @11:52:12.60 15.2psi after pressurize @11:52:12.63 Archive.in :compress @11:52:12.75 Archive.seek :empty @11:52:27.43 28.6psi after compress @11:52:38.05 29.8psi after compress (stable) @11:52:38.08 Archive.in :gulp @11:52:38.19 Archive.seek :topVent @11:52:44.49 30.1psi after gulp @11:52:50.57 30.1psi after gulp (stable) @11:52:50.69 Archive stroke #3 of 8 @11:52:50.83 Archive.in :pressurize @11:52:50.93 Archive.seek :pressurized @11:52:52.67 Reached 29.5 of 30.4psi after pumping 3626uL (2.10 strokes) @11:52:52.70 Archive.in :default @11:52:52.80 Archive.hold @11:52:52.91 Handoff.select :waste @11:53:00.23 delay 1:00 @11:54:00.25 Handoff.select :blocked @11:54:23.04 Handoff.select :waste @11:54:23.18 0.4psi at evacEnd @11:54:23.22 Air.seek :fullVent @11:54:24.68 Archive.in :fast @11:54:24.87 Archive.seek :topVent @11:54:26.53 Seawater evacuated, Pressure above/below filter released. @11:54:26.69 Lysis1.in :slow @11:54:26.81 Lysis1.seek 1050uL @11:54:56.87 delay 5 seconds @11:55:01.89 Handoff.select :blocked @11:55:02.15 Lysis1.seek 450uL @11:56:02.17 delay 3 seconds @11:56:05.28 Air.in mix with 1.40048 parts Lysis1 @11:56:06.03 Air.seek :evac @11:56:06.09 Lysis1.seek 300uL @11:56:21.28 Lysis1.seek :empty @11:56:51.50 Lysis buffer delivered @37.9psi @11:56:51.52 Polling Can every 2:00 @11:56:52.09 PuckHeater.configure :default #ambient 26.1C @11:56:52.17 PuckHeater.seek 79.6C @11:57:37.98 PuckHeater.hold 2:00,79.6C @11:59:37.73 Polling Can every 2:00 @11:59:38.29 PuckHeater.configure :default #ambient 26.2C @11:59:38.37 PuckHeater.hold 30 seconds,78.5C @12:00:08.48 Polling Can every 2:00 @12:00:09.04 PuckHeater.configure :default #ambient 26.2C @12:00:09.17 PuckHeater.hold 30 seconds,78C @12:00:39.28 Polling Can every 2:00 @12:00:39.84 PuckHeater.hold 10:00,77.5C @12:10:38.28 Lysis complete. Syringe pressure @46.5psi @12:10:38.31 Archive syringe pumping up to 5 strokes to reach 40psi @12:10:38.35 Archive stroke #1 of 5 @12:10:38.49 Archive.in :pressurize @12:10:38.59 Archive.seek :pressurized @12:10:38.81 Reached 46.5 of 40.0psi after pumping 0uL (0.00 strokes) @12:10:38.83 Archive.in :default @12:10:38.97 Archive.hold @12:10:39.12 delay 2 seconds @12:10:41.15 Archive.in :fast @12:10:41.25 Archive.seek :full @12:10:41.51 Lysis1.seek 138uL @12:10:55.42 delay 1 second @12:10:56.45 Archive.seek :full @12:10:56.49 Lysis1.seek :empty @12:11:10.46 Handoff.select :blocked @12:11:10.60 Initial air pressure @45.6psi @12:11:10.69 Lysis1.in :extract @12:11:10.86 Lysis1.seek 600uL,nil @12:11:12.86 Archive syringe pumping up to 6 strokes to reach 25psi @12:11:12.91 Archive stroke #1 of 6 @12:11:13.14 Archive.in :pressurize @12:11:13.26 Archive.seek :pressurized @12:11:25.83 10.1psi after pressurize @12:11:31.83 10.0psi after pressurize (stable) @12:11:31.86 Archive.in :compress @12:11:32.04 Archive.seek :empty @12:11:42.27 Archive.in :default @12:11:42.37 Archive.hold @12:11:42.66 Initial 600uL lysate @18.4psi @12:11:42.68 Handoff.select :blocked @12:11:42.83 Initial air pressure @19.1psi @12:11:42.94 Lysis1.seek 825uL,nil @12:11:44.93 Archive syringe pumping up to 6 strokes to reach 17psi @12:11:44.99 Archive stroke #1 of 6 @12:11:45.18 Archive.in :compress @12:11:45.32 Archive.seek :empty @12:11:45.51 Archive.in :default @12:11:45.59 Archive.hold @12:11:45.93 Recovered 825uL lysate @14.2psi @12:11:45.96 Air.in :halfSpeed @12:11:46.67 Air.seek :fullVent @12:11:48.93 Puck Top Vented, bottom @4.7psi @12:11:48.96 Handoff.select :waste @12:11:49.19 delay 5 seconds @12:11:54.28 Handoff open to waste, bottom @0.2psi @12:11:54.31 Air.in :default @12:11:54.42 Air.seek :evac @12:11:55.87 Lysis2.in :DAmix @12:11:56.04 Lysis1.in :DAmix @12:11:56.64 Lysis2.seek 700uL @12:11:56.73 Lysis1.seek 575uL @12:12:31.88 delay 5 seconds @12:12:36.90 Handoff.select SPR @12:12:37.10 SPR.command "start 2 14" @12:12:38.23 SPR stabilized @12:12:38.42 delay 10 seconds @12:12:48.45 SPR.command "start 2 0" @12:12:51.09 Waiting up to 11:00 for SPR to enter state 1 @12:14:48.74 SPR.command "hold_on" @12:14:50.14 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:14:50.82 Lysis2.seek 400uL @12:14:50.88 Lysis1.seek 275uL @12:15:33.03 delay 15 seconds @12:15:48.06 SPR.command "hold_off" @12:15:50.79 Waiting up to 38:00 for SPR to enter state 7 @12:30:11.00 SPRsummary:4.78RIU,13.5RIU,86.6RIU,110RIU,high,183ng/L,288ng/L after 2795s @12:41:41.40 SPR analysis completed @12:41:47.94 Ended log spr23aug1211h.spr.bz2 @12:41:47.96 SPR.command "stats_off" @12:41:48.69 Handoff.select :close @12:41:48.86 Gate.power :heat,:OFF @12:41:48.89 Gate.power :cartridge,:OFF @12:41:49.05 PinPort.in :unlocking @12:41:49.14 PinPort.seek :clear @12:41:51.12 Carousel.in :default @12:41:51.28 Carousel.home.seek 28 @12:41:51.65 Plunge.seek :locked @12:41:52.58 Archive.in :unlocking @12:41:52.77 Archive.seek :clear @12:41:52.85 Air.in :unlocking @12:41:53.09 Air.seek :clear @12:41:53.24 Lysis1.in :unlocking @12:41:53.43 Lysis1.seek :clear @12:41:53.49 Lysis2.in :unlocking @12:41:53.67 Lysis2.seek :clear @12:41:57.58 Gate.power :motor,:OFF @12:41:57.60 Gate.power :core,:OFF @12:41:57.64 Gate.power :analytic1,:OFF @12:41:57.68 Gate.power :analytic2,:OFF @12:41:57.71 Gate.power :main,:OFF @12:41:57.73 Safely stopped and ready to power off @12:44:32.66 -> exit @12:44:32.69 Session Ended @12:46:30.17 Session Begun @12:46:31.88 -> can Can@12:46:31, 26.1C, 44% humidity, 14.4psia, 14.876V, 0.088A, 5.602Ah, 1.31W @12:47:43.27 -> exit @12:47:43.30 Session Ended @14:30:31.04 Session Begun @14:30:41.73 -> ESP.ready! @14:30:41.79 Gate.power :sampler,:OFF,limit to 3500mA @14:30:41.81 Gate.power :main,:ON,limit to 5000mA @14:30:41.85 Gate.power :analytic1,:ON,limit to 5000mA @14:30:41.88 Gate.power :analytic2,:ON,limit to 5000mA @14:30:41.97 Powering core bus @14:30:44.32 SPR Module 1, FW Rev. B, FPGA Rev. C @14:30:44.74 Archive.configure :default @14:30:44.86 Air.configure :default @14:30:44.98 Lysis1.configure :default @14:30:45.07 Lysis2.configure :default @14:30:45.18 Plunge.configure :default @14:30:45.30 SamplePump.configure :default @14:30:45.42 Toroid.configure :default @14:30:45.53 Carousel.configure :default @14:30:45.66 Pressure.configure :default @14:30:45.77 ExtraPump.configure :default @14:30:45.86 PinPort.configure :default @14:30:45.95 All elf controllers running firmware version 4.84 @14:30:45.98 Gate.power :motor,:ON,limit to 5000mA @14:30:46.40 Archive.home.jog 0 @14:30:46.99 Carousel.home.jog 0 @14:30:47.06 Toroid.home.jog 0 @14:30:47.11 Plunge.home.jog 0 @14:30:47.16 Lysis1.home.jog 0 @14:30:47.21 PinPort.home.jog 0 @14:30:47.25 Air.home.jog 0 @14:30:47.39 Lysis2.home.jog 0 @14:30:48.16 Archive.in :homing @14:30:48.48 Archive.jog 1388 @14:30:48.78 PinPort.in :homing @14:30:48.85 Plunge.in :homing @14:30:48.91 Lysis1.in :homing @14:30:48.98 Lysis2.in :homing @14:30:49.08 Air.in :homing @14:30:49.13 Toroid.in :homing @14:30:49.46 Plunge.jog 1576 @14:30:49.54 PinPort.jog -107 @14:30:49.58 Lysis1.jog 1389 @14:30:49.65 Lysis2.jog 1395 @14:30:49.69 Toroid.jog 591 @14:30:49.73 Air.jog 1371 @14:30:50.21 Archive.setRawPosition! 50 @14:30:50.24 PinPort.setRawPosition! 0 @14:30:50.37 Archive.seek :home @14:30:50.41 PinPort.seek :home @14:30:50.64 PinPort.in :default @14:30:50.76 Archive.in :default @14:30:51.05 Plunge.setRawPosition! 50 @14:30:51.18 Lysis2.setRawPosition! 50 @14:30:51.21 Lysis1.setRawPosition! 50 @14:30:51.69 Plunge.seek :home @14:30:51.93 Lysis2.seek :home @14:30:52.02 Lysis1.seek :home @14:30:52.27 Toroid.setRawPosition! 0 @14:30:52.31 Plunge.in :default @14:30:52.39 Air.setRawPosition! 50 @14:30:52.52 Toroid.seek :home @14:30:52.62 Air.seek :home @14:30:52.81 Lysis2.in :default @14:30:52.84 Lysis1.in :default @14:30:52.88 Toroid.in :default @14:30:53.20 Air.in :default @14:30:53.56 Toroid.seek :clear @14:30:55.21 Carousel.in :default @14:30:55.37 Carousel.home.seek 28 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 28 Pressure = Absolute Pressure (Leak) Sensor @14psia PinPort = Handoff Pin Port (PP) clear @14:31:08.84 -> SPR.start @14:31:10.05 SPR.command "read_sequences" @14:31:10.66 SPR.command "stats_on" # @14:31:11.54 SPR.start /var/log/esp/spr23aug1214h.spr.bz2 @14:31:19.34 -> delay2 :delay2 @14:31:42.14 -> SPR.command "start 3 0" @14:31:42.17 SPR.command "start 3 0" @14:31:55.01 -> delay '15:00' @14:31:55.05 delay 15:00 @14:47:31.41 -> SPR.command "start 2 14" @14:47:31.44 SPR.command "start 2 14" @14:47:40.90 -> SPR.stop @14:47:46.91 Ended log spr23aug1214h.spr.bz2 @14:47:46.96 SPR.command "stats_off" @14:47:49.40 -> delay 10 @14:47:49.43 delay 10 seconds @14:48:05.39 -> Cmd.stop @14:48:05.60 Carousel.in :default @14:48:05.76 Carousel.home.seek 28 @14:48:06.14 Lysis1.in :fast @14:48:06.23 Lysis1.seek :locked @14:48:08.79 Plunge.seek :locked @14:48:10.16 Lysis1.in :unlocking @14:48:10.37 Lysis1.seek :clear @14:48:11.89 Gate.power :motor,:OFF @14:48:11.92 Gate.power :core,:OFF @14:48:11.96 Gate.power :analytic1,:OFF @14:48:11.99 Gate.power :analytic2,:OFF @14:48:12.03 Gate.power :main,:OFF @14:48:12.06 Safely stopped and ready to power off :STOPPED @14:48:17.71 -> exit @14:48:17.74 Session Ended @16:29:02.25 Session Begun @16:29:12.55 -> espclient errera @16:29:12.58 NoMethodError in espclient -- undefined method `espclient' for CmdServer::Client:Class @16:29:17.68 -> can Can@16:29:17, 24.6C, 45% humidity, 14.3psia, 14.874V, 0.094A, 6.019Ah, 1.39W @16:30:04.15 -> exit @16:30:04.17 Session Ended @04:38:06.23PDT13-Aug-23 Session Begun @04:38:08.82 -> can Can@04:38:08, 25.7C, 46% humidity, 14.4psia, 14.871V, 0.088A, 7.004Ah, 1.31W @04:39:39.11 -> exit @04:39:39.14 Session Ended @06:50:07.98 Session Begun @06:50:09.80 -> can Can@06:50:09, 25.2C, 45% humidity, 14.4psia, 14.871V, 0.088A, 7.182Ah, 1.31W @06:58:12.48 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @06:58:12.86 Gate.power :sampler,:OFF,limit to 3500mA @06:58:12.97 Gate.power :main,:ON,limit to 5000mA @06:58:13.08 Gate.power :analytic1,:ON,limit to 5000mA @06:58:13.14 Gate.power :analytic2,:ON,limit to 5000mA @06:58:13.27 Powering core bus @06:58:15.61 SPR Module 1, FW Rev. B, FPGA Rev. C @06:58:16.08 Archive.configure :default @06:58:16.25 Air.configure :default @06:58:16.41 Lysis1.configure :default @06:58:16.53 Lysis2.configure :default @06:58:16.68 Plunge.configure :default @06:58:16.84 SamplePump.configure :default @06:58:17.01 Toroid.configure :default @06:58:17.19 Carousel.configure :default @06:58:17.87 Pressure.configure :default @06:58:18.18 ExtraPump.configure :default @06:58:18.29 PinPort.configure :default @06:58:18.40 All elf controllers running firmware version 4.84 @06:58:18.44 Gate.power :motor,:ON,limit to 5000mA @06:58:18.97 Air.home.jog 0 @06:58:19.14 Lysis1.home.jog 0 @06:58:19.22 Archive.home.jog 0 @06:58:20.11 Carousel.home.jog 0 @06:58:20.16 Plunge.home.jog 0 @06:58:20.26 Toroid.home.jog 0 @06:58:20.33 Lysis2.home.jog 0 @06:58:20.40 PinPort.home.jog 0 @06:58:21.13 Air.in :homing @06:58:21.27 Lysis1.in :homing @06:58:21.37 Archive.in :homing @06:58:21.82 Air.jog 1369 @06:58:21.84 Archive.jog 1387 @06:58:21.91 Lysis1.jog 1388 @06:58:22.30 Toroid.in :homing @06:58:22.35 Plunge.in :homing @06:58:22.40 PinPort.in :homing @06:58:22.49 Lysis2.in :homing @06:58:22.74 PinPort.jog -108 @06:58:22.78 Plunge.jog 1571 @06:58:22.85 Toroid.jog 587 @06:58:22.89 Lysis2.jog 1394 @06:58:23.37 PinPort.setRawPosition! 0 @06:58:23.42 Lysis1.setRawPosition! 50 @06:58:23.45 Archive.setRawPosition! 50 @06:58:23.50 Air.setRawPosition! 50 @06:58:23.73 PinPort.seek :home @06:58:23.78 Lysis1.seek :home @06:58:23.83 Archive.seek :home @06:58:23.91 Air.seek :home @06:58:24.30 PinPort.in :default @06:58:24.49 Lysis2.setRawPosition! 50 @06:58:24.56 Lysis1.in :default @06:58:24.59 Archive.in :default @06:58:24.65 Plunge.setRawPosition! 50 @06:58:24.70 Air.in :default @06:58:24.92 Lysis2.seek :home @06:58:25.18 Plunge.seek :home @06:58:25.37 Toroid.setRawPosition! 0 @06:58:25.45 Toroid.seek :home @06:58:25.63 Plunge.in :default @06:58:25.66 Lysis2.in :default @06:58:25.71 Toroid.in :default @06:58:26.22 Toroid.seek :clear @06:58:27.87 Carousel.in :default @06:58:28.04 Carousel.home.seek 28 @06:58:28.37 Selecting Cartridge 27 @06:58:29.33 Carousel.seek 27 @06:58:29.95 Lysis1.in :fast @06:58:30.04 Lysis1.seek :locked @06:58:32.71 Plunge.seek :locked @06:58:32.91 Air.in :fast @06:58:33.01 Air.seek :locked @06:58:34.63 Carousel.reconfigure :locked @06:58:34.82 Powering cartridge bus @06:58:37.50 Gate.setIR 1750,5000,0,6 @06:58:37.64 Gate.setIR 0,5000,0,6 @06:58:37.75 Gate.power :cartridge,:OFF @06:58:38.33 Mounted archive Cartridge @06:58:38.78 Preparing to filter cartridge 27 @06:58:39.08 Gate.power :sampler,:ON @06:58:39.21 Priming sample loop w/150ml, bypass w/50ml @06:58:39.24 SamplePump.setPosition! 0ml @06:58:39.33 Bypass.open @06:58:40.93 Intake.open @06:58:41.78 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:58:44.39 Vacuum tare is 56% of full scale @06:58:44.61 Pressure.in :default @06:58:44.72 Pressure.hold @06:58:45.00 Waiting for external sampler to finish priming... @06:58:45.04 delayUntil "07:02:09.10" @07:02:09.16 SamplePump.in :prime @07:02:09.28 SamplePump.seek 50ml,2:53 @07:03:10.31 Pressure.stop @07:03:10.40 SamplePump.setPosition! 0ml @07:03:10.47 Exhaust.open @07:03:11.92 Bypass.close @07:03:13.54 Pressure.in :default @07:03:13.63 Pressure.hold @07:03:13.98 SamplePump.in :prime @07:03:14.09 SamplePump.seek 150ml,7:39 @07:06:17.05 Pressure.stop @07:06:17.17 Bypass.open @07:06:17.93 Intake.close @07:06:18.78 Toroid.in :hold @07:06:18.88 Toroid.seek :crack @07:06:20.46 Toroid.seek :cartridge @07:06:21.60 Toroid.in :default @07:06:21.70 Toroid.seek :clear @07:06:23.77 Pressurizing cartridge to 26psi above 13.9psia @07:06:23.80 SamplePump.setPosition! 0ml @07:06:23.88 Intake.open @07:06:24.57 Bypass.close @07:06:25.29 Exhaust.close @07:06:26.15 Pressure.in :default @07:06:26.26 Pressure.hold @07:06:26.53 SamplePump.in :charge @07:06:26.66 SamplePump.seek 2.3ml,42 seconds @07:06:35.57 Pressure.stop @07:06:35.67 Intake.close @07:06:36.41 Pressurized cartridge with 0.353ml to 26.2psi @07:06:36.43 Isolated @33.6psia (19.6psi) @07:06:36.45 Verifying cartridge holds at least 0.65..0.85 of 33.6psia @07:06:36.51 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:06:41.23 Sampling 1000.0ml @07:06:41.25 SamplePump.setPosition! 0ml @07:06:41.36 Exhaust.open @07:06:42.12 Intake.open @07:06:42.96 Pressure.in :default @07:06:43.09 Pressure.hold @07:06:43.27 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:06:43.50 SamplePump.in :sample @07:06:43.61 SamplePump.seek 1000ml,1:23:51 @07:06:46.64 Cartridge 27 filtering 1/1000ml at 0.62ml/s with 23.2psi vacuum @07:07:46.64 Cartridge 27 filtering 33/1000ml at 0.52ml/s with 21.0psi vacuum @07:08:46.64 Cartridge 27 filtering 62/1000ml at 0.48ml/s with 19.9psi vacuum @07:09:46.65 Cartridge 27 filtering 89/1000ml at 0.43ml/s with 14.3psi vacuum @07:10:46.64 Cartridge 27 filtering 115/1000ml at 0.44ml/s with 20.5psi vacuum @07:11:46.65 Cartridge 27 filtering 139/1000ml at 0.38ml/s with 12.4psi vacuum @07:12:46.64 Cartridge 27 filtering 161/1000ml at 0.41ml/s with 22.7psi vacuum @07:13:46.65 Cartridge 27 filtering 182/1000ml at 0.32ml/s with 28.9psi vacuum @07:14:46.67 Cartridge 27 filtering 202/1000ml at 0.27ml/s with 24.8psi vacuum @07:15:46.63 Cartridge 27 filtering 220/1000ml at 0.30ml/s with 29.6psi vacuum @07:16:46.64 Cartridge 27 filtering 237/1000ml at 0.30ml/s with 18.7psi vacuum 1.6m deep (17.0psia) @07:17:46.64 Cartridge 27 filtering 254/1000ml at 0.24ml/s with 27.9psi vacuum @07:18:46.78 Cartridge 27 filtering 269/1000ml at 0.24ml/s with 23.0psi vacuum @07:19:46.64 Cartridge 27 filtering 283/1000ml at 0.22ml/s with 18.2psi vacuum @07:20:46.64 Cartridge 27 filtering 296/1000ml at 0.21ml/s with 19.6psi vacuum @07:20:52.53 Flow averaged 0.35ml/s over 14:10 @07:20:52.60 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @07:20:53.22 Relieved excess vacuum in .7 seconds @07:20:53.25 SamplePump.seek 1000ml,59:03 @07:21:34.91 Flow averaged 0.22ml/s over 42 seconds @07:21:34.99 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @07:21:35.63 Relieved excess vacuum in .7 seconds @07:21:35.67 SamplePump.seek 1000ml,58:16 @07:21:46.63 Cartridge 27 filtering 309/1000ml at 0.20ml/s with 26.5psi vacuum @07:21:55.41 Flow averaged 0.22ml/s over 20 seconds @07:21:55.49 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @07:21:56.10 Relieved excess vacuum in .7 seconds @07:21:56.14 SamplePump.seek 1000ml,57:55 @07:22:13.14 Flow averaged 0.22ml/s over 18 seconds @07:22:13.22 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @07:22:13.82 Relieved excess vacuum in .7 seconds @07:22:13.89 SamplePump.seek 1000ml,57:36 @07:22:26.79 Flow averaged 0.22ml/s over 13 seconds @07:22:26.87 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @07:22:27.47 Relieved excess vacuum in .7 seconds @07:22:27.52 SamplePump.seek 1000ml,57:22 @07:22:37.78 Flow averaged 0.21ml/s over 11 seconds @07:22:37.86 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi @07:22:38.47 Relieved excess vacuum in .7 seconds @07:22:38.52 SamplePump.seek 1000ml,57:11 @07:22:46.72 Cartridge 27 filtering 321/1000ml at 0.26ml/s with 15.5psi vacuum @07:22:49.23 Flow averaged 0.20ml/s over 11 seconds @07:22:49.31 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @07:22:49.93 Relieved excess vacuum in .7 seconds @07:22:49.97 SamplePump.seek 1000ml,57:01 @07:22:57.29 Flow estimate reduced to 0.16ml/s @07:22:57.33 Intake.close @07:22:57.39 Exhaust.close @07:22:57.74 Toroid.in :hold @07:22:57.84 Toroid.seek :bypass @07:22:59.53 Toroid.in :default @07:22:59.64 Toroid.seek :clear @07:23:01.27 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @07:23:01.30 Pressure.stop @07:23:01.58 Sampled 323.6ml @07:23:02.29 Plunge.seek :reagent @07:23:03.13 Sample loop closed. Ready to Deliver Reagents @07:23:03.16 Archive.seek :sealed @07:23:04.74 Gate.power :sampler,:OFF @07:23:07.15 Archive.seek :evac @07:23:21.23 delay 1:00 @07:24:21.27 Archive.seek :clear @07:24:38.78 RNAlater soak for 10:00 @07:34:38.82 Archive.seek :tVent @07:34:41.30 delay 1 second @07:34:42.33 Archive.seek :evac @07:34:57.88 delay 10 seconds @07:35:07.92 Archive.seek :tVent @07:35:23.47 delay 1 second @07:35:24.50 Archive.seek :evac @07:35:40.05 delay 10 seconds @07:35:50.09 Sample preserved and evacuated @07:35:50.26 Selecting Cartridge 26 @07:35:50.53 Carousel.in :default @07:35:50.69 Carousel.home.seek 27 @07:35:51.13 Archive.in :unlocking @07:35:51.33 Archive.seek :clear @07:35:51.44 Air.in :unlocking @07:35:51.65 Air.seek :clear @07:35:55.89 Lysis1.in :unlocking @07:35:56.59 Lysis1.seek :clear @07:35:56.70 Plunge.in :unlocking @07:35:56.81 Plunge.seek :clear @07:35:58.25 Carousel.seek 26 @07:35:58.84 Lysis1.in :fast @07:35:58.94 Lysis1.seek :locked @07:36:01.65 Plunge.in :default @07:36:01.78 Plunge.seek :locked @07:36:01.93 Air.in :fast @07:36:02.03 Air.seek :locked @07:36:03.63 Carousel.reconfigure :locked @07:36:03.84 Powering cartridge bus @07:36:06.50 Gate.setIR 1750,5000,0,6 @07:36:06.64 Gate.setIR 0,5000,0,6 @07:36:06.73 PuckHeater.configure :default #ambient 26.2C @07:36:06.82 Gate.power :heat,:ON,limit to 5000mA @07:36:07.51 Mounted lysis Cartridge board#Q05059 @07:36:07.57 Using default cartridge heat loss curve for HeatingAccelInCarousel @07:36:07.94 Preparing to filter cartridge 26 @07:36:08.21 Gate.power :sampler,:ON @07:36:08.35 Priming sample loop w/150ml, bypass w/50ml @07:36:08.37 SamplePump.setPosition! 0ml @07:36:08.45 Bypass.open @07:36:09.99 Intake.open @07:36:10.79 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:36:10.92 Cartridge::Sampler::Warning in FILTERING -- Delta Pressure @-8.8psi @07:36:13.39 Vacuum tare is 56% of full scale @07:36:13.59 Pressure.in :default @07:36:13.69 Pressure.hold @07:36:13.95 Waiting for external sampler to finish priming... @07:36:13.97 delayUntil "07:39:38.24" @07:39:38.29 SamplePump.in :prime @07:39:38.39 SamplePump.seek 50ml,2:53 @07:40:39.43 Pressure.stop @07:40:39.52 SamplePump.setPosition! 0ml @07:40:39.59 Exhaust.open @07:40:41.08 Bypass.close @07:40:43.23 Pressure.in :default @07:40:43.33 Pressure.hold @07:40:43.67 SamplePump.in :prime @07:40:43.78 SamplePump.seek 150ml,7:39 @07:43:46.06 Pressure.stop @07:43:46.17 Bypass.open @07:43:46.94 Intake.close @07:43:47.80 Toroid.in :hold @07:43:47.90 Toroid.seek :crack @07:43:49.48 Toroid.seek :cartridge @07:43:50.62 Toroid.in :default @07:43:50.73 Toroid.seek :clear @07:43:52.79 Pressurizing cartridge to 26psi above 13.6psia @07:43:52.81 SamplePump.setPosition! 0ml @07:43:52.90 Intake.open @07:43:53.59 Bypass.close @07:43:54.30 Exhaust.close @07:43:55.19 Pressure.in :default @07:43:55.29 Pressure.hold @07:43:55.55 SamplePump.in :charge @07:43:55.67 SamplePump.seek 2.3ml,42 seconds @07:44:09.59 Pressure.stop @07:44:09.68 Intake.close @07:44:10.47 Pressurized cartridge with 0.874ml to 26.6psi @07:44:10.48 Isolated @34.1psia (20.6psi) @07:44:10.51 Verifying cartridge holds at least 0.65..0.85 of 34.1psia @07:44:10.57 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:44:15.27 Sampling 50.0ml @07:44:15.29 SamplePump.setPosition! 0ml @07:44:15.38 Exhaust.open @07:44:16.12 Intake.open @07:44:16.98 Pressure.in :default @07:44:17.16 Pressure.hold @07:44:17.40 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:44:17.62 SamplePump.in :sample @07:44:18.16 SamplePump.seek 50ml,4:41 @07:44:20.75 Cartridge 26 filtering 1/50ml at 0.61ml/s with 18.3psi vacuum @07:45:20.76 Cartridge 26 filtering 32/50ml at 0.50ml/s with 22.6psi vacuum @07:45:57.91 Pressure.stop @07:45:58.09 Intake.close @07:45:58.19 Exhaust.close @07:45:58.41 Toroid.in :hold @07:45:58.51 Toroid.seek :bypass @07:46:00.17 Toroid.in :default @07:46:00.27 Toroid.seek :clear @07:46:01.92 Sampled 50.0ml @07:46:03.67 SPR.command "read_sequences" @07:46:04.15 SPR.command "stats_on" @07:46:04.35 SPR.command "start 0 1" @07:46:05.04 SPR.start /var/log/esp/spr23aug1307h.spr.bz2 @07:46:05.11 Gate.power :sampler,:OFF @07:46:07.50 Handoff.select :waste @07:46:14.24 Syringe tare is 17% of full scale @07:46:14.28 PinPort.in :locking @07:46:14.39 PinPort.seek :locked @07:46:16.98 Archive.in :fast @07:46:17.09 Lysis2.in :fast @07:46:17.19 Lysis1.seek 1350uL @07:46:17.29 Archive.seek :full @07:46:17.47 Lysis2.seek 950uL @07:46:22.21 Plunge.seek :reagent @07:46:23.15 Evacuating puck with filter bubble point of 50psi @07:46:23.18 PinPort.seek :locked @07:46:23.32 Handoff.select :waste @07:46:29.94 Air syringe pumping up to 1 strokes to reach 8psi @07:46:29.95 Air stroke #1 of 1 @07:46:30.10 Air.in :pressurize @07:46:30.22 Air.seek :pressurized @07:46:36.00 0.9psi after pressurize @07:46:36.03 Air.in :compress @07:46:36.14 Air.seek :evac @07:46:39.18 1.0psi after compress @07:46:49.80 0.9psi after compress (stable) @07:46:49.82 Air.in :default @07:46:49.93 Air.hold @07:46:50.24 at 0.8psi Opened to waste @07:46:50.26 Handoff.select :blocked @07:46:50.44 Archive syringe pumping up to 8 strokes to reach 50psi @07:46:50.45 Archive stroke #1 of 8 @07:46:50.60 Archive.in :pressurize @07:46:50.69 Archive.seek :pressurized @07:47:03.21 2.4psi after pressurize @07:47:08.81 2.3psi after pressurize (stable) @07:47:08.84 Archive.in :compress @07:47:09.03 Archive.seek :empty @07:47:23.69 16.5psi after compress @07:47:36.97 19.0psi after compress (stable) @07:47:37.02 Archive.in :gulp @07:47:37.16 Archive.seek :topVent @07:47:43.45 19.3psi after gulp @07:47:49.09 19.3psi after gulp (stable) @07:47:49.10 Archive stroke #2 of 8 @07:47:49.25 Archive.in :pressurize @07:47:49.35 Archive.seek :pressurized @07:48:01.90 19.0psi after pressurize @07:48:13.05 23.2psi after pressurize (stable) @07:48:13.09 Archive.in :compress @07:48:13.62 Archive.seek :empty @07:48:28.44 33.3psi after compress @07:48:39.56 34.8psi after compress (stable) @07:48:39.59 Archive.in :gulp @07:48:39.71 Archive.seek :topVent @07:48:46.05 35.0psi after gulp @07:48:51.65 34.9psi after gulp (stable) @07:48:51.66 Archive stroke #3 of 8 @07:48:51.82 Archive.in :pressurize @07:48:51.91 Archive.seek :pressurized @07:49:04.46 34.4psi after pressurize @07:49:20.57 36.7psi after pressurize (stable) @07:49:20.59 Archive.in :compress @07:49:20.71 Archive.seek :empty @07:49:35.36 47.4psi after compress @07:49:38.90 Reached 47.9 of 50.0psi after pumping 5190uL (3.00 strokes) @07:49:38.92 Archive.in :default @07:49:39.07 Archive.hold @07:49:39.17 Handoff.select :waste @07:49:49.92 delay 1:00 @07:50:49.95 Handoff.select :blocked @07:51:13.16 Handoff.select :waste @07:51:13.32 0.5psi at evacEnd @07:51:13.33 Handoff.select :blocked @07:51:13.50 Archive.in :fast @07:51:13.60 Archive.seek :topVent @07:51:27.32 Stable @0.5psi after Handoff blocked @07:51:27.36 Archive syringe pumping up to 8 strokes to reach 31psi @07:51:27.39 Archive stroke #1 of 8 @07:51:27.53 Archive.in :pressurize @07:51:27.62 Archive.seek :pressurized @07:51:40.15 3.0psi after pressurize @07:51:40.19 Archive.in :compress @07:51:40.30 Archive.seek :empty @07:51:54.97 14.2psi after compress @07:52:05.61 14.6psi after compress (stable) @07:52:05.64 Archive.in :gulp @07:52:05.76 Archive.seek :topVent @07:52:12.06 14.7psi after gulp @07:52:17.66 14.6psi after gulp (stable) @07:52:17.68 Archive stroke #2 of 8 @07:52:17.83 Archive.in :pressurize @07:52:17.92 Archive.seek :pressurized @07:52:30.45 14.6psi after pressurize @07:52:30.49 Archive.in :compress @07:52:30.62 Archive.seek :empty @07:52:39.20 Reached 23.6 of 30.5psi after pumping 3328uL (1.92 strokes) @07:52:39.22 Archive.in :default @07:52:39.34 Archive.hold @07:52:39.45 Handoff.select :waste @07:52:51.19 delay 1:00 @07:53:51.21 Handoff.select :blocked @07:54:13.96 Handoff.select :waste @07:54:14.12 0.3psi at evacEnd @07:54:14.14 Air.seek :fullVent @07:54:15.62 Archive.in :fast @07:54:15.71 Archive.seek :topVent @07:54:22.38 Seawater evacuated, Pressure above/below filter released. @07:54:22.52 Lysis1.in :slow @07:54:22.61 Lysis1.seek 1050uL @07:54:52.94 delay 5 seconds @07:54:57.97 Handoff.select :blocked @07:54:58.24 Lysis1.seek 450uL @07:55:58.21 delay 3 seconds @07:56:01.32 Air.in mix with 1.40048 parts Lysis1 @07:56:02.08 Air.seek :evac @07:56:02.15 Lysis1.seek 300uL @07:56:17.30 Lysis1.seek :empty @07:56:47.43 Lysis buffer delivered @9.5psi @07:56:47.45 Polling Can every 2:00 @07:56:48.04 PuckHeater.configure :default #ambient 25.9C @07:56:48.12 PuckHeater.seek 79.6C @07:57:34.88 PuckHeater.hold 2:00,79.6C @07:59:34.86 Polling Can every 2:00 @07:59:35.42 PuckHeater.configure :default #ambient 25.9C @07:59:35.50 PuckHeater.hold 30 seconds,78.6C @08:00:05.70 Polling Can every 2:00 @08:00:06.25 PuckHeater.configure :default #ambient 25.9C @08:00:06.34 PuckHeater.hold 30 seconds,78C @08:00:36.58 Polling Can every 2:00 @08:00:37.13 PuckHeater.configure :default #ambient 25.9C @08:00:37.22 PuckHeater.hold 10:00,77.5C @08:01:14.24 Session Begun @08:01:29.87 -> showlog -200 @08:05:58.54 -> exit @08:05:58.57 Session Ended @08:10:36.60 Lysis complete. Syringe pressure @13.7psi @08:10:36.63 Archive syringe pumping up to 5 strokes to reach 40psi @08:10:36.66 Archive stroke #1 of 5 @08:10:36.81 Archive.in :pressurize @08:10:36.90 Archive.seek :pressurized @08:10:49.43 13.7psi after pressurize @08:11:28.54 26.1psi after pressurize (stable) @08:11:28.57 Archive.in :compress @08:11:28.69 Archive.seek :empty @08:11:39.12 Reached 40.4 of 40.0psi after pumping 1637uL (0.95 strokes) @08:11:39.15 Archive.in :default @08:11:39.26 Archive.hold @08:11:39.36 delay 2 seconds @08:11:41.39 Archive.in :fast @08:11:41.49 Archive.seek :full @08:11:48.38 Lysis1.seek 138uL @08:12:02.29 delay 1 second @08:12:03.33 Archive.seek :full @08:12:03.41 Lysis1.seek :empty @08:12:17.36 Handoff.select :blocked @08:12:17.52 Initial air pressure @40.2psi @08:12:17.65 Lysis1.in :extract @08:12:17.84 Lysis1.seek 600uL,nil @08:12:23.29 Archive syringe pumping up to 6 strokes to reach 25psi @08:12:23.31 Archive stroke #1 of 6 @08:12:23.52 Archive.in :default @08:12:23.59 Archive.hold @08:12:23.86 Initial 600uL lysate @15.2psi @08:12:23.88 Handoff.select :blocked @08:12:24.02 Initial air pressure @15.2psi @08:12:24.11 Lysis1.seek 825uL,nil @08:12:24.31 Archive syringe pumping up to 6 strokes to reach 17psi @08:12:24.33 Archive stroke #1 of 6 @08:12:24.59 Archive.in :pressurize @08:12:24.74 Archive.seek :pressurized @08:12:34.36 Reached 14.5 of 17.0psi after pumping 1080uL (0.62 strokes) @08:12:34.39 Archive.in :default @08:12:34.50 Archive.hold @08:12:34.90 Archive syringe pumping up to 6 strokes to reach 17psi @08:12:34.91 Archive stroke #1 of 6 @08:12:35.16 Archive.in :pressurize @08:12:35.28 Archive.seek :pressurized @08:12:37.16 Archive.in :default @08:12:37.25 Archive.hold @08:12:37.76 Recovered 827uL lysate @14.4psi @08:12:37.80 Air.in :halfSpeed @08:12:37.90 Air.seek :fullVent @08:12:40.15 Puck Top Vented, bottom @5.2psi @08:12:40.17 Handoff.select :waste @08:12:40.35 delay 5 seconds @08:12:45.44 Handoff open to waste, bottom @0.4psi @08:12:45.47 Air.in :default @08:12:45.58 Air.seek :evac @08:12:47.06 Lysis2.in :DAmix @08:12:47.22 Lysis1.in :DAmix @08:12:47.85 Lysis2.seek 700uL @08:12:47.92 Lysis1.seek 577uL @08:13:23.08 delay 5 seconds @08:13:28.10 Handoff.select SPR @08:13:28.26 SPR.command "start 2 14" @08:13:28.97 SPR stabilized @08:13:29.21 delay 10 seconds @08:13:39.23 SPR.command "start 2 0" @08:13:42.85 Waiting up to 11:00 for SPR to enter state 1 @08:15:40.94 SPR.command "hold_on" @08:15:42.34 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @08:15:43.07 Lysis2.seek 400uL @08:15:43.11 Lysis1.seek 277uL @08:16:25.20 delay 15 seconds @08:16:40.23 SPR.command "hold_off" @08:16:43.00 Waiting up to 38:00 for SPR to enter state 7 @08:31:03.17 SPRsummary:19.4RIU,21.2RIU,142RIU,198RIU,high,52.4ng/L,106ng/L after 2850s @08:42:33.61 SPR analysis completed @08:42:40.95 Ended log spr23aug1307h.spr.bz2 @08:42:40.98 SPR.command "stats_off" @08:42:41.70 Archive.stop @08:42:41.89 Handoff.select :close @08:42:42.06 Gate.power :heat,:OFF @08:42:42.08 Gate.power :cartridge,:OFF @08:42:42.21 PinPort.in :unlocking @08:42:42.30 PinPort.seek :clear @08:42:44.25 Carousel.in :default @08:42:44.41 Carousel.home.seek 26 @08:42:44.80 Plunge.seek :locked @08:42:45.73 Archive.in :unlocking @08:42:45.92 Archive.seek :clear @08:42:45.99 Air.in :unlocking @08:42:46.23 Air.seek :clear @08:42:46.36 Lysis1.in :unlocking @08:42:46.55 Lysis1.seek :clear @08:42:46.60 Lysis2.in :unlocking @08:42:46.76 Lysis2.seek :clear @08:42:51.17 Gate.power :motor,:OFF @08:42:51.22 Gate.power :core,:OFF @08:42:51.26 Gate.power :analytic1,:OFF @08:42:51.29 Gate.power :analytic2,:OFF @08:42:51.32 Gate.power :main,:OFF @08:42:51.36 Safely stopped and ready to power off @08:44:01.32 -> ESP.ready!; SPR.start; delay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.command “start 2 14"; SPR.stop; delay 10; Cmd.stop @08:44:01.36 SyntaxError in errera -- compile error errera:3: Invalid char `\342' in expression errera:3: Invalid char `\200' in expression errera:3: Invalid char `\234' in expression errera:3: syntax error, unexpected tINTEGER, expecting $end ...ay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.co... ^ @08:45:54.58 -> ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay '15:00';SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @08:45:54.66 Gate.power :sampler,:OFF,limit to 3500mA @08:45:54.69 Gate.power :main,:ON,limit to 5000mA @08:45:54.73 Gate.power :analytic1,:ON,limit to 5000mA @08:45:54.76 Gate.power :analytic2,:ON,limit to 5000mA @08:45:54.87 Powering core bus @08:45:57.21 SPR Module 1, FW Rev. B, FPGA Rev. C @08:45:57.65 Archive.configure :default @08:45:57.77 Air.configure :default @08:45:57.86 Lysis1.configure :default @08:45:57.94 Lysis2.configure :default @08:45:58.07 Plunge.configure :default @08:45:58.19 SamplePump.configure :default @08:45:58.30 Toroid.configure :default @08:45:58.42 Carousel.configure :default @08:45:58.54 Pressure.configure :default @08:45:58.66 ExtraPump.configure :default @08:45:58.75 PinPort.configure :default @08:45:58.84 All elf controllers running firmware version 4.84 @08:45:58.87 Gate.power :motor,:ON,limit to 5000mA @08:45:59.27 Archive.home.jog 0 @08:45:59.36 Air.home.jog 0 @08:46:00.16 Carousel.home.jog 0 @08:46:00.26 Toroid.home.jog 0 @08:46:00.29 Lysis2.home.jog 0 @08:46:00.34 Lysis1.home.jog 0 @08:46:00.38 PinPort.home.jog 0 @08:46:00.42 Plunge.home.jog 0 @08:46:01.23 Archive.in :homing @08:46:01.42 Air.in :homing @08:46:01.56 Archive.jog 1387 @08:46:01.61 Air.jog 1370 @08:46:01.91 Toroid.in :homing @08:46:01.97 Plunge.in :homing @08:46:02.01 Lysis1.in :homing @08:46:02.10 PinPort.in :homing @08:46:02.14 Lysis2.in :homing @08:46:02.41 Plunge.jog 1576 @08:46:02.51 Toroid.jog 587 @08:46:02.54 Lysis1.jog 1390 @08:46:02.59 PinPort.jog -107 @08:46:02.63 Lysis2.jog 1393 @08:46:03.07 Archive.setRawPosition! 50 @08:46:03.10 Air.setRawPosition! 50 @08:46:03.23 Archive.seek :home @08:46:03.27 Air.seek :home @08:46:03.47 PinPort.setRawPosition! 0 @08:46:03.56 PinPort.seek :home @08:46:03.72 Air.in :default @08:46:03.75 Archive.in :default @08:46:03.79 PinPort.in :default @08:46:04.05 Plunge.setRawPosition! 50 @08:46:04.09 Lysis1.setRawPosition! 50 @08:46:04.21 Plunge.seek :home @08:46:04.68 Lysis1.seek :home @08:46:05.05 Lysis2.setRawPosition! 50 @08:46:05.07 Toroid.setRawPosition! 0 @08:46:05.24 Toroid.seek :home @08:46:05.26 Lysis2.seek :home @08:46:05.55 Toroid.in :default @08:46:05.58 Plunge.in :default @08:46:05.74 Lysis1.in :default @08:46:05.85 Lysis2.in :default @08:46:06.21 Toroid.seek :clear @08:46:07.86 Carousel.in :default @08:46:08.02 Carousel.home.seek 26 @08:46:09.46 SPR.command "read_sequences" @08:46:09.53 SPR.command "stats_on" @08:46:10.32 delay 2 seconds @08:46:11.11 SPR.start /var/log/esp/spr23aug1308h.spr.bz2 @08:46:12.34 SPR.command "start 3 0" @08:46:13.17 delay 15:00 @09:01:13.18 SPR.command "start 2 14" @09:01:19.87 Ended log spr23aug1308h.spr.bz2 @09:01:19.89 SPR.command "stats_off" @09:01:20.21 delay 10 seconds @09:01:30.41 Carousel.in :default @09:01:30.56 Carousel.home.seek 26 @09:01:31.40 Lysis1.in :fast @09:01:31.50 Lysis1.seek :locked @09:01:34.05 Plunge.seek :locked @09:01:35.45 Lysis1.in :unlocking @09:01:35.64 Lysis1.seek :clear @09:01:37.19 Gate.power :motor,:OFF @09:01:37.22 Gate.power :core,:OFF @09:01:37.25 Gate.power :analytic1,:OFF @09:01:37.29 Gate.power :analytic2,:OFF @09:01:37.31 Gate.power :main,:OFF @09:01:37.36 Safely stopped and ready to power off :STOPPED @09:03:24.52 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @09:03:24.75 Gate.power :sampler,:OFF,limit to 3500mA @09:03:24.80 Gate.power :main,:ON,limit to 5000mA @09:03:24.83 Gate.power :analytic1,:ON,limit to 5000mA @09:03:24.87 Gate.power :analytic2,:ON,limit to 5000mA @09:03:24.97 Powering core bus @09:03:27.33 SPR Module 1, FW Rev. B, FPGA Rev. C @09:03:27.77 Archive.configure :default @09:03:27.90 Air.configure :default @09:03:27.99 Lysis1.configure :default @09:03:28.09 Lysis2.configure :default @09:03:28.22 Plunge.configure :default @09:03:28.34 SamplePump.configure :default @09:03:28.47 Toroid.configure :default @09:03:28.59 Carousel.configure :default @09:03:28.70 Pressure.configure :default @09:03:28.83 ExtraPump.configure :default @09:03:28.92 PinPort.configure :default @09:03:29.05 All elf controllers running firmware version 4.84 @09:03:29.08 Gate.power :motor,:ON,limit to 5000mA @09:03:29.31 Archive.home.jog 0 @09:03:30.20 Carousel.home.jog 0 @09:03:30.26 Toroid.home.jog 0 @09:03:30.32 Lysis2.home.jog 0 @09:03:30.35 Air.home.jog 0 @09:03:30.40 PinPort.home.jog 0 @09:03:30.44 Plunge.home.jog 0 @09:03:30.48 Lysis1.home.jog 0 @09:03:31.51 Archive.in :homing @09:03:31.80 Archive.jog 1387 @09:03:31.88 Toroid.in :homing @09:03:31.93 Lysis2.in :homing @09:03:31.97 Air.in :homing @09:03:32.07 PinPort.in :homing @09:03:32.11 Plunge.in :homing @09:03:32.19 Lysis1.in :homing @09:03:32.55 Toroid.jog 581 @09:03:32.63 Lysis2.jog 1395 @09:03:32.64 Air.jog 1365 @09:03:32.69 Plunge.jog 1568 @09:03:32.75 PinPort.jog -107 @09:03:32.83 Lysis1.jog 1386 @09:03:33.81 Archive.setRawPosition! 50 @09:03:33.89 Archive.seek :home @09:03:34.03 PinPort.setRawPosition! 0 @09:03:34.12 PinPort.seek :home @09:03:34.27 Lysis2.setRawPosition! 50 @09:03:34.32 Air.setRawPosition! 50 @09:03:34.35 PinPort.in :default @09:03:34.39 Archive.in :default @09:03:34.64 Lysis2.seek :home @09:03:34.70 Air.seek :home @09:03:34.95 Toroid.setRawPosition! 0 @09:03:34.97 Plunge.setRawPosition! 50 @09:03:35.06 Lysis1.setRawPosition! 50 @09:03:35.24 Plunge.seek :home @09:03:35.30 Toroid.seek :home @09:03:35.35 Lysis1.seek :home @09:03:35.68 Lysis2.in :default @09:03:35.71 Air.in :default @09:03:35.78 Toroid.in :default @09:03:36.05 Plunge.in :default @09:03:36.07 Lysis1.in :default @09:03:36.50 Toroid.seek :clear @09:03:38.14 Carousel.in :default @09:03:38.31 Carousel.home.seek 26 @09:03:38.60 Selecting Cartridge 25 @09:03:38.95 Carousel.seek 25 @09:03:39.58 Lysis1.in :fast @09:03:39.68 Lysis1.seek :locked @09:03:42.34 Plunge.seek :locked @09:03:42.53 Air.in :fast @09:03:42.62 Air.seek :locked @09:03:44.23 Carousel.reconfigure :locked @09:03:44.43 Powering cartridge bus @09:03:47.11 Gate.setIR 1750,5000,0,6 @09:03:47.24 Gate.setIR 0,5000,0,6 @09:03:47.34 Gate.power :cartridge,:OFF @09:03:47.92 Mounted archive Cartridge @09:03:48.30 Preparing to filter cartridge 25 @09:03:48.57 Gate.power :sampler,:ON @09:03:48.69 Priming sample loop w/150ml, bypass w/50ml @09:03:48.72 SamplePump.setPosition! 0ml @09:03:48.79 Bypass.open @09:03:50.31 Intake.open @09:03:51.13 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:03:53.72 Vacuum tare is 56% of full scale @09:03:53.92 Pressure.in :default @09:03:54.01 Pressure.hold @09:03:54.27 Waiting for external sampler to finish priming... @09:03:54.30 delayUntil "09:07:18.59" @09:07:18.64 SamplePump.in :prime @09:07:18.75 SamplePump.seek 50ml,2:53 @09:08:19.80 Pressure.stop @09:08:19.89 SamplePump.setPosition! 0ml @09:08:19.96 Exhaust.open @09:08:21.46 Bypass.close @09:08:23.10 Pressure.in :default @09:08:23.19 Pressure.hold @09:08:23.53 SamplePump.in :prime @09:08:23.64 SamplePump.seek 150ml,7:39 @09:11:26.05 Pressure.stop @09:11:26.17 Bypass.open @09:11:26.95 Intake.close @09:11:27.84 Toroid.in :hold @09:11:27.94 Toroid.seek :crack @09:11:29.54 Toroid.seek :cartridge @09:11:30.69 Toroid.in :default @09:11:30.78 Toroid.seek :clear @09:11:32.83 Pressurizing cartridge to 26psi above 13.5psia @09:11:32.86 SamplePump.setPosition! 0ml @09:11:32.95 Intake.open @09:11:33.65 Bypass.close @09:11:34.37 Exhaust.close @09:11:35.23 Pressure.in :default @09:11:35.33 Pressure.hold @09:11:35.60 SamplePump.in :charge @09:11:35.70 SamplePump.seek 2.3ml,42 seconds @09:11:44.42 Pressure.stop @09:11:44.52 Intake.close @09:11:45.28 Pressurized cartridge with 0.336ml to 26.1psi @09:11:45.30 Isolated @34.4psia (20.9psi) @09:11:45.33 Verifying cartridge holds at least 0.65..0.85 of 34.4psia @09:11:45.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:11:50.01 Cartridge pressure fell 1% to 34.1psia @09:11:50.14 Sampling 1000.0ml @09:11:50.17 SamplePump.setPosition! 0ml @09:11:50.26 Exhaust.open @09:11:51.04 Intake.open @09:11:51.97 Pressure.in :default @09:11:52.06 Pressure.hold @09:11:52.24 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @09:11:52.47 SamplePump.in :sample @09:11:52.58 SamplePump.seek 1000ml,1:23:51 @09:11:55.60 Cartridge 25 filtering 1/1000ml at 0.59ml/s with 31.3psi vacuum @09:12:55.61 Cartridge 25 filtering 34/1000ml at 0.54ml/s with 25.9psi vacuum @09:13:55.61 Cartridge 25 filtering 63/1000ml at 0.48ml/s with 29.6psi vacuum @09:14:55.61 Cartridge 25 filtering 90/1000ml at 0.38ml/s with 24.5psi vacuum @09:15:55.61 Cartridge 25 filtering 117/1000ml at 0.39ml/s with 14.2psi vacuum @09:16:55.61 Cartridge 25 filtering 141/1000ml at 0.43ml/s with 19.4psi vacuum @09:17:55.61 Cartridge 25 filtering 165/1000ml at 0.33ml/s with 19.2psi vacuum @09:18:55.61 Cartridge 25 filtering 187/1000ml at 0.40ml/s with 29.9psi vacuum @09:19:55.61 Cartridge 25 filtering 207/1000ml at 0.32ml/s with 23.9psi vacuum @09:20:55.61 Cartridge 25 filtering 227/1000ml at 0.29ml/s with 14.5psi vacuum @09:21:55.61 Cartridge 25 filtering 245/1000ml at 0.24ml/s with 23.4psi vacuum @09:22:55.61 Cartridge 25 filtering 262/1000ml at 0.25ml/s with 27.6psi vacuum @09:23:08.41 SamplePump.stop @09:23:08.51 Intake.close @09:23:08.64 Exhaust.close @09:23:08.89 Toroid.in :hold @09:23:09.00 Toroid.seek :bypass @09:23:10.71 Toroid.in :default @09:23:10.81 Toroid.seek :clear @09:23:12.45 Pressure.stop @09:23:12.97 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only -0.4psi vacuum after 268.4ml @09:23:13.02 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 268.39ml" @09:23:15.84 Gate.power :sampler,:OFF @09:27:36.22 Session Begun @09:27:42.16 -> showlog -200 @09:32:29.21 Session Begun @09:33:14.26 -> loop{puts Sampler.psi} @09:33:20.64 -> Cmd.resume @09:33:20.74 Resuming Thread (suspended since 09:03:48) @09:33:20.80 Preparing to filter cartridge 25 @09:33:21.41 Gate.power :sampler,:ON @09:33:21.78 Toroid.in :hold @09:33:21.95 Toroid.seek :cartridge @09:33:24.17 Toroid.in :default @09:33:24.37 Toroid.seek :clear @09:33:26.40 Sampling 731.6ml @09:33:26.47 SamplePump.setPosition! 0ml @09:33:26.77 Exhaust.open @09:33:27.62 Intake.open @09:33:28.91 Pressure.in :default @09:33:29.21 Pressure.hold @09:33:29.69 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @09:33:30.18 Waiting for external sampler to finish priming... @09:33:30.27 delayUntil "09:36:51.47" @09:33:35.73 UserAbort in n -- User Abort!! @09:36:51.54 SamplePump.seek 731.6ml,1:01:29 @09:36:54.66 Cartridge 25 filtering 1/732ml at 0.42ml/s with 23.9psi vacuum @09:37:05.48 -> loop{puts Sampler.psi} @09:37:36.25 SamplePump.stop @09:37:36.42 Intake.close @09:37:36.48 Exhaust.close @09:37:36.94 Toroid.in :hold @09:37:37.18 Toroid.seek :bypass @09:37:39.19 Toroid.in :default @09:37:39.39 Toroid.seek :clear @09:37:41.22 Pressure.stop @09:37:42.32 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only -0.0psi vacuum after 17.9ml @09:37:42.36 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 17.87ml" @09:37:45.12 Gate.power :sampler,:OFF @09:37:45.68 UserAbort in n -- User Abort!! @09:49:00.75 -> exit @09:49:00.78 Session Ended @09:49:50.83 -> can Can@09:49:50, 26.6C, 44% humidity, 14.4psia, 14.855V, 0.367A, 9.008Ah, 5.45W @09:50:16.44 -> Can.query! @09:50:16.50 NoMethodError in errera -- undefined method `query!' for Can:Class @09:50:39.84 Session Begun @09:50:45.55 -> Power.sampler :ON @09:50:45.61 Gate.power :sampler,:ON true @09:51:45.32 -> BACsampler.prime @09:51:45.46 Priming sample loop w/150ml, bypass w/50ml @09:51:45.49 SamplePump.setPosition! 0ml @09:51:45.64 Bypass.open @09:51:47.28 Intake.open @09:51:48.83 Pressure.in :default @09:51:48.96 Pressure.hold @09:51:49.31 Waiting for external sampler to finish priming... @09:51:49.34 delayUntil "09:54:15.65" @09:54:15.71 SamplePump.in :prime @09:54:15.84 SamplePump.seek 50ml,2:53 @09:54:19.04 SamplePump.stop @09:54:19.17 Intake.close @09:54:19.29 Bypass.close @09:54:20.18 Pressure.stop @09:54:20.32 Cartridge::Sampler::Leaky in n -- Serious Leak or Stall -- 0.82ml/s made only -0.0psi vacuum after 2.3ml @09:54:27.56 -> >can @09:54:27.62 SyntaxError in errera -- compile error errera:8: syntax error, unexpected '>' @09:54:50.32 -> Can Can@09:54:19, 26.7C, 44% humidity, 14.4psia, 14.842V, 0.607A, 9.035Ah, 9.01W @09:55:30.79 -> Power.sampler true @09:56:53.04 -> require 'vacuumCart' true @09:56:57.36 -> refillSampleLines @09:56:57.42 Refilling sample & bypass lines with fluid. @09:56:58.40 Toroid.seek :clear @09:56:58.55 Carousel.in :default @09:56:58.91 Carousel.home.seek 25 @09:56:59.80 Carousel.reconfigure :locked @09:57:00.10 Session Begun @09:57:00.40 Powering cartridge bus @09:57:03.08 Gate.setIR 1750,5000,0,6 @09:57:03.26 Gate.setIR 0,5000,0,6 @09:57:03.45 Gate.power :cartridge,:OFF @09:57:04.08 Mounted archive Cartridge @09:57:04.21 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @09:57:04.29 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @09:57:04.31 Bypass.open @09:57:05.21 Intake.open @09:57:05.99 SamplePump.setPosition! 0ml @09:57:06.18 SamplePump.in :default @09:57:06.33 SamplePump.seek 30ml @09:57:07.79 -> Can.query! @09:57:07.88 NoMethodError in brent -- undefined method `query!' for Can:Class @09:57:25.22 -> can! @09:57:25.27 Polling Can every 2:00 Can@09:57:25, 26.7C, 44% humidity, 14.4psia, 14.810V, 0.966A, 9.057Ah, 14.31W @09:57:43.24 Bypass.close @09:57:44.02 Exhaust.open @09:57:44.89 SamplePump.seek 55ml @09:57:49.24 -> upsync #<#::NoThread:0xb6ceddc0> @09:57:56.19 -> _.finish #<#::NoThread:0xb6ceddc0> @09:58:14.18 -> list method :upsync def upsync Log.upload.finish end @09:58:15.77 delay 1 second @09:58:16.80 Intake.close @09:58:16.94 Exhaust.close @09:58:17.88 Sampling Loop filled with water @09:58:23.04 -> exit @09:58:23.07 Session Ended @09:58:30.39 -> Power.sampler true @09:58:38.43 Session Begun @09:58:39.71 -> 2.times{refillSampleLines} @09:58:39.77 Refilling sample & bypass lines with fluid. @09:58:40.70 Toroid.seek :clear @09:58:40.86 Carousel.in :default @09:58:41.11 Carousel.home.seek 25 @09:58:41.62 Carousel.reconfigure :locked @09:58:41.88 Powering cartridge bus @09:58:44.58 Gate.setIR 1750,5000,0,6 @09:58:44.72 Gate.setIR 0,5000,0,6 @09:58:44.87 Gate.power :cartridge,:OFF @09:58:45.48 Mounted archive Cartridge @09:58:45.62 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @09:58:46.74 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @09:58:46.76 Bypass.open @09:58:47.64 Intake.open @09:58:48.39 SamplePump.setPosition! 0ml @09:58:48.58 SamplePump.seek 30ml @09:59:00.19 -> Gate.queryCan! @09:59:00.23 Polling Can every 2:00 Can@09:59:00, 26.7C, 44% humidity, 14.4psia, 14.812V, 0.969A, 9.077Ah, 14.35W @09:59:25.47 Bypass.close @09:59:26.27 Exhaust.open @09:59:27.13 SamplePump.seek 55ml @09:59:57.96 delay 1 second @09:59:59.00 Intake.close @09:59:59.14 Exhaust.close @10:00:00.00 Sampling Loop filled with water @10:00:00.03 Refilling sample & bypass lines with fluid. @10:00:01.08 Toroid.seek :clear @10:00:01.25 Carousel.in :default @10:00:01.46 Carousel.home.seek 25 @10:00:01.95 Carousel.reconfigure :locked @10:00:02.80 Powering cartridge bus @10:00:05.47 Gate.setIR 1750,5000,0,6 @10:00:05.61 Gate.setIR 0,5000,0,6 @10:00:05.76 Gate.power :cartridge,:OFF @10:00:06.37 Mounted archive Cartridge @10:00:06.50 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @10:00:06.56 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @10:00:06.61 Bypass.open @10:00:07.50 Intake.open @10:00:08.26 SamplePump.setPosition! 0ml @10:00:08.46 SamplePump.seek 30ml @10:00:45.37 Bypass.close @10:00:46.13 Exhaust.open @10:00:47.04 SamplePump.seek 55ml @10:01:17.87 delay 1 second @10:01:18.89 Intake.close @10:01:18.95 Exhaust.close @10:01:19.86 Sampling Loop filled with water 2 @10:01:26.11 -> BACsampler.prime @10:01:26.30 Priming sample loop w/150ml, bypass w/50ml @10:01:26.33 SamplePump.setPosition! 0ml @10:01:26.51 Bypass.open @10:01:28.14 Intake.open @10:01:29.82 Pressure.in :default @10:01:30.02 Pressure.hold @10:01:30.35 SamplePump.in :prime @10:01:30.50 SamplePump.seek 50ml,2:53 @10:02:31.54 Pressure.stop @10:02:31.66 SamplePump.setPosition! 0ml @10:02:31.77 Exhaust.open @10:02:33.25 Bypass.close @10:02:34.93 Pressure.in :default @10:02:35.10 Pressure.hold @10:02:35.50 SamplePump.in :prime @10:02:35.65 SamplePump.seek 150ml,7:39 @10:05:37.98 Pressure.stop BACsampler @10:06:37.55 -> Power {analytic1: true, analytic2: true, cartridge: false, core: true, heat: false, main: true, motor: true, sampler: true} @10:07:00.84 -> archiveEvac2AUV @10:07:01.09 Plunge.seek :reagent @10:07:01.99 Sample loop closed. Ready to Deliver Reagents @10:07:02.07 Archive.seek :sealed @10:07:06.08 Archive.seek :evac @10:07:12.75 Cmd::Error in nadia -- Not Processing @10:07:12.84 UserAbort in nadia -- User Abort!! @10:07:20.23 delay 1:00 @10:07:44.35 -> Power.sampler :OFF @10:07:44.39 Gate.power :sampler,:OFF false @10:08:20.26 Archive.seek :clear @10:08:37.88 RNAlater soak for 10:00 @10:10:11.87 -> progress >>> Thread[:MAIN] is sleeping <<< @13:42:00PDT11-Aug-23 Waiting for espclients... >>> Thread["detached from errera"] is sleeping <<< @09:03:38 Selecting Cartridge 25 >>> Thread[:FILTERING] is suspended <<< @09:03:48 Preparing to filter cartridge 25 @09:03:48 Priming sample loop w/150ml, bypass w/50ml @09:03:51 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:11:32 Pressurizing cartridge to 26psi above 13.5psia @09:11:38 Sample Pump (SP) pressureOutOfBounds at 0.34ml @09:11:45 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:11:50 Sampling 1000.0ml @09:23:05 Sample Pump (SP) pressureOutOfBounds at 268.39ml @09:33:26 Sampling 731.6ml @09:37:36 Sample Pump (SP) pressureOutOfBounds at 17.87ml >>> Thread["nadia"] is sleeping <<< @10:07:09 User Abort!! >>> Thread["n"] is sleeping <<< @09:51:45 Priming sample loop w/150ml, bypass w/50ml @09:54:15 Sample Pump (SP) pressureOutOfBounds at 2.33ml @09:56:57 Refilling sample & bypass lines with fluid. @09:57:04 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @09:58:17 Sampling Loop filled with water @09:58:39 Refilling sample & bypass lines with fluid. @09:58:45 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @09:59:59 Sampling Loop filled with water @10:00:00 Refilling sample & bypass lines with fluid. @10:00:06 Waiting for n to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @10:01:19 Sampling Loop filled with water @10:01:26 Priming sample loop w/150ml, bypass w/50ml @10:07:01 Sample loop closed. Ready to Deliver Reagents @10:08:37 RNAlater soak for 10:00 @10:11:29.00 -> upsync #<#::NoThread:0xb6ceddc0> @10:11:52.35 -> exit @10:11:52.38 Session Ended @10:14:08.04 Session Begun @10:14:16.35 -> Log.uploadCmd = "upsync" upsync @10:14:33.00 -> upsync.finish @10:14:35.64 upsync @10:14:35.68 NameError in uploadLog -- uninitialized constant Class::Email @10:14:35.83 Thread::ChildDied in brent -- :uploadLog @10:15:13.64 -> Log.uploadCmd=nil @10:16:58.27 -> Cart.type :archiveHiBiomass_bac @10:18:37.92 Archive.seek :tVent @10:18:40.47 delay 1 second @10:18:41.50 Archive.seek :evac @10:18:57.15 delay 10 seconds @10:19:07.19 Archive.seek :tVent @10:19:22.77 delay 1 second @10:19:23.80 Archive.seek :evac @10:19:39.39 delay 10 seconds @10:19:49.43 Sample preserved and evacuated @10:20:25.64 -> exit @10:20:25.67 Session Ended @10:20:45.49 Session Ended @10:21:34.90 -> MainThread.exit @10:21:35.06 Exhaust.close @10:21:35.14 Intake.close # @10:21:36.06 Session Ended @10:21:36.10 Session Ended nohup: {fire: No such file or directory @10:24:51.16PDT13-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @10:24:53.30 >BREAK< ESP3 Gateway boot v1.43 for core bus @10:24:53.32 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @10:24:56.74 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @10:24:57.38 Sea pressure (Abs) tare is 6% of full scale @10:25:06.69 Polling Can every 2:00 @10:25:06.74 Waiting for espclients... @10:25:07.36 Session Begun @10:25:44.52 -> start {fire :microSPR_phyto} # @10:25:44.89 Gate.power :sampler,:OFF,limit to 3500mA @10:25:44.91 Gate.power :analytic1,:ON,limit to 5000mA @10:25:44.93 Gate.power :analytic2,:ON,limit to 5000mA @10:25:45.57 SPR Module 1, FW Rev. B, FPGA Rev. C @10:25:45.79 Archive.configure :default @10:25:45.87 Archive.hold @10:25:46.87 Archive.configure :default @10:25:47.36 Air.configure :default @10:25:47.53 Session Begun @10:25:47.62 Air.hold @10:25:47.86 Air.configure :default @10:25:48.15 Lysis1.configure :default @10:25:48.27 Lysis1.hold @10:25:48.38 Lysis1.configure :default @10:25:48.56 Lysis2.configure :default @10:25:48.77 Plunge.configure :default @10:25:48.88 Plunge.hold @10:25:49.03 Plunge.configure :default @10:25:49.26 SamplePump.configure :default @10:25:49.48 Toroid.configure :default @10:25:49.60 Toroid.hold @10:25:49.72 Toroid.configure :default @10:25:49.92 Carousel.configure :default @10:25:50.04 Carousel.hold @10:25:50.15 Carousel.configure :default @10:25:50.36 Pressure.configure :default @10:25:50.57 ExtraPump.configure :default @10:25:50.75 PinPort.configure :default @10:25:50.85 All elf controllers running firmware version 4.84 @10:25:51.80 Toroid.seek :clear @10:25:51.93 Carousel.in :default @10:25:52.11 Carousel.home.seek 25 @10:25:52.57 Carousel.reconfigure :locked @10:25:52.80 Powering cartridge bus @10:25:55.46 Gate.setIR 1750,5000,0,6 @10:25:55.60 Gate.setIR 0,5000,0,6 @10:25:55.71 Gate.power :cartridge,:OFF @10:25:56.30 Mounted archive Cartridge @10:25:56.41 Selecting Cartridge 24 @10:25:56.70 Carousel.in :default @10:25:56.88 Carousel.home.seek 25 @10:25:57.28 Archive.in :unlocking @10:25:57.48 Archive.seek :clear @10:25:57.55 Air.in :unlocking @10:25:57.82 Air.seek :clear @10:26:02.14 Lysis1.in :unlocking @10:26:02.35 Lysis1.seek :clear @10:26:02.47 Plunge.in :unlocking @10:26:03.09 Plunge.seek :clear @10:26:04.55 Carousel.seek 24 @10:26:05.19 Lysis1.in :fast @10:26:05.30 Lysis1.seek :locked @10:26:07.98 Plunge.in :default @10:26:08.12 Plunge.seek :locked @10:26:08.31 Air.in :fast @10:26:08.42 Air.seek :locked @10:26:10.04 Carousel.reconfigure :locked @10:26:10.26 Powering cartridge bus @10:26:12.94 Gate.setIR 1750,5000,0,6 @10:26:13.07 Gate.setIR 0,5000,0,6 @10:26:13.17 PuckHeater.configure :default @10:26:13.30 Gate.power :heat,:ON,limit to 5000mA @10:26:14.06 Mounted lysis Cartridge board#Q05042 @10:26:14.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:26:14.53 Preparing to filter cartridge 24 @10:26:14.82 Gate.power :sampler,:ON @10:26:14.96 Priming sample loop w/150ml, bypass w/50ml @10:26:14.98 SamplePump.setPosition! 0ml @10:26:15.12 Exhaust.close @10:26:15.26 Bypass.open @10:26:16.86 Intake.open @10:26:17.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:26:20.27 Vacuum tare is 56% of full scale @10:26:20.49 Pressure.in :default @10:26:20.61 Pressure.hold @10:26:20.89 Waiting for external sampler to finish priming... @10:26:20.92 delayUntil "10:29:44.85" @10:27:03.80 Session Begun @10:27:10.19 -> Log.uploadCmd=nil @10:27:17.64 -> showlog -100 @10:29:44.91 SamplePump.in :prime @10:29:45.08 SamplePump.seek 50ml,2:53 @10:30:46.10 Pressure.stop @10:30:46.23 SamplePump.setPosition! 0ml @10:30:46.33 Exhaust.open @10:30:47.86 Bypass.close @10:30:49.52 Pressure.in :default @10:30:49.66 Pressure.hold @10:30:50.03 SamplePump.in :prime @10:30:50.67 SamplePump.seek 150ml,7:39 @10:33:53.02 Pressure.stop @10:33:53.17 Bypass.open @10:33:53.97 Intake.close @10:33:54.88 Toroid.in :hold @10:33:55.04 Toroid.seek :crack @10:33:56.64 Toroid.seek :cartridge @10:33:57.80 Toroid.in :default @10:33:57.93 Toroid.seek :clear @10:34:00.00 Pressurizing cartridge to 26psi above 13.5psia @10:34:00.03 SamplePump.setPosition! 0ml @10:34:00.17 Intake.open @10:34:00.92 Bypass.close @10:34:01.73 Exhaust.close @10:34:02.60 Pressure.in :default @10:34:02.73 Pressure.hold @10:34:03.07 SamplePump.in :charge @10:34:03.22 SamplePump.seek 2.3ml,42 seconds @10:34:16.46 Pressure.stop @10:34:16.59 Intake.close @10:34:17.43 Pressurized cartridge with 0.788ml to 26.1psi @10:34:17.45 Isolated @33.9psia (20.4psi) @10:34:17.50 Verifying cartridge holds at least 0.65..0.85 of 33.9psia @10:34:17.63 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:34:22.24 Cartridge pressure fell 1% to 33.7psia @10:34:22.40 Sampling 50.0ml @10:34:22.44 SamplePump.setPosition! 0ml @10:34:22.59 Exhaust.open @10:34:23.37 Intake.open @10:34:24.26 Pressure.in :default @10:34:24.39 Pressure.hold @10:34:24.61 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @10:34:24.88 SamplePump.in :sample @10:34:25.05 SamplePump.seek 50ml,4:41 @10:34:28.00 Cartridge 24 filtering 1/50ml at 0.62ml/s with 19.3psi vacuum @10:35:28.01 Cartridge 24 filtering 33/50ml at 0.52ml/s with 22.6psi vacuum @10:36:03.33 Pressure.stop @10:36:03.54 Intake.close @10:36:03.64 Exhaust.close @10:36:03.96 Toroid.in :hold @10:36:04.09 Toroid.seek :bypass @10:36:06.25 Toroid.in :default @10:36:06.39 Toroid.seek :clear @10:36:08.05 Sampled 50.0ml @10:36:09.84 SPR.command "read_sequences" @10:36:10.57 SPR.command "stats_on" @10:36:10.79 SPR.command "start 0 1" @10:36:11.26 Gate.power :sampler,:OFF @10:36:11.49 SPR.start /var/log/esp/spr23aug1310h.spr.bz2 @10:36:13.42 Handoff.select :waste @10:36:20.21 Syringe tare is 18% of full scale @10:36:20.29 PinPort.in :locking @10:36:20.42 PinPort.seek :locked @10:36:23.06 Archive.in :fast @10:36:23.13 Lysis2.in :fast @10:36:23.39 Lysis1.seek 1350uL @10:36:23.48 Archive.seek :full @10:36:23.67 Lysis2.seek 950uL @10:36:28.51 Plunge.seek :reagent @10:36:29.50 Evacuating puck with filter bubble point of 50psi @10:36:29.54 PinPort.seek :locked @10:36:29.74 Handoff.select :waste @10:36:36.36 Air syringe pumping up to 1 strokes to reach 8psi @10:36:36.38 Air stroke #1 of 1 @10:36:37.21 Air.in :pressurize @10:36:37.35 Air.seek :pressurized @10:36:43.15 1.7psi after pressurize @10:36:43.20 Air.in :compress @10:36:43.39 Air.seek :evac @10:36:46.46 1.1psi after compress @10:36:57.09 0.9psi after compress (stable) @10:36:57.11 Air.in :default @10:36:57.28 Air.hold @10:36:57.64 at 0.8psi Opened to waste @10:36:57.66 Handoff.select :blocked @10:36:57.90 Archive syringe pumping up to 8 strokes to reach 50psi @10:36:57.91 Archive stroke #1 of 8 @10:36:58.10 Archive.in :pressurize @10:36:58.27 Archive.seek :pressurized @10:37:10.85 1.8psi after pressurize @10:37:16.46 1.8psi after pressurize (stable) @10:37:16.54 Archive.in :compress @10:37:16.66 Archive.seek :empty @10:37:31.36 16.2psi after compress @10:37:41.97 17.3psi after compress (stable) @10:37:42.05 Archive.in :gulp @10:37:42.18 Archive.seek :topVent @10:37:48.60 17.8psi after gulp @10:37:54.25 17.8psi after gulp (stable) @10:37:54.26 Archive stroke #2 of 8 @10:37:54.47 Archive.in :pressurize @10:37:54.57 Archive.seek :pressurized @10:38:07.18 17.7psi after pressurize @10:38:12.79 17.6psi after pressurize (stable) @10:38:12.83 Archive.in :compress @10:38:13.02 Archive.seek :empty @10:38:27.78 31.1psi after compress @10:38:41.88 32.4psi after compress (stable) @10:38:41.95 Archive.in :gulp @10:38:42.09 Archive.seek :topVent @10:38:48.42 33.0psi after gulp @10:38:54.02 33.0psi after gulp (stable) @10:38:54.04 Archive stroke #3 of 8 @10:38:54.23 Archive.in :pressurize @10:38:54.37 Archive.seek :pressurized @10:39:06.94 32.6psi after pressurize @10:39:12.56 32.5psi after pressurize (stable) @10:39:12.65 Archive.in :compress @10:39:12.79 Archive.seek :empty @10:39:27.49 45.0psi after compress @10:39:31.40 Reached 45.1 of 50.0psi after pumping 5190uL (3.00 strokes) @10:39:31.44 Archive.in :default @10:39:31.65 Archive.hold @10:39:31.81 Handoff.select :waste @10:39:42.08 delay 1:00 @10:40:42.11 Handoff.select :blocked @10:41:04.89 Handoff.select :waste @10:41:05.11 0.5psi at evacEnd @10:41:05.13 Handoff.select :blocked @10:41:05.39 Archive.in :fast @10:41:05.52 Archive.seek :topVent @10:41:19.28 Stable @0.8psi after Handoff blocked @10:41:19.31 Archive syringe pumping up to 8 strokes to reach 31psi @10:41:19.34 Archive stroke #1 of 8 @10:41:19.60 Archive.in :pressurize @10:41:19.75 Archive.seek :pressurized @10:41:32.32 0.9psi after pressurize @10:41:32.39 Archive.in :compress @10:41:32.52 Archive.seek :empty @10:41:47.27 13.7psi after compress @10:41:58.41 14.8psi after compress (stable) @10:41:58.50 Archive.in :gulp @10:41:58.62 Archive.seek :topVent @10:42:04.96 15.1psi after gulp @10:42:10.60 15.0psi after gulp (stable) @10:42:10.61 Archive stroke #2 of 8 @10:42:10.82 Archive.in :pressurize @10:42:10.96 Archive.seek :pressurized @10:42:23.57 14.9psi after pressurize @10:42:23.63 Archive.in :compress @10:42:23.82 Archive.seek :empty @10:42:38.55 27.5psi after compress @10:42:49.15 28.4psi after compress (stable) @10:42:49.19 Archive.in :gulp @10:42:49.41 Archive.seek :topVent @10:42:55.74 28.9psi after gulp @10:43:01.10 Reached 28.3 of 30.8psi after pumping 3460uL (2.00 strokes) @10:43:01.12 Archive.in :default @10:43:01.29 Archive.hold @10:43:01.45 Handoff.select :waste @10:43:13.74 delay 1:00 @10:44:13.77 Handoff.select :blocked @10:44:36.54 Handoff.select :waste @10:44:36.73 0.9psi at evacEnd @10:44:36.76 Air.seek :fullVent @10:44:38.29 Archive.in :fast @10:44:38.43 Archive.seek :topVent @10:44:38.56 Seawater evacuated, Pressure above/below filter released. @10:44:38.73 Lysis1.in :slow @10:44:38.85 Lysis1.seek 1050uL @10:45:08.96 delay 5 seconds @10:45:14.00 Handoff.select :blocked @10:45:14.28 Lysis1.seek 450uL @10:46:14.31 delay 3 seconds @10:46:17.46 Air.in mix with 1.40048 parts Lysis1 @10:46:18.17 Air.seek :evac @10:46:18.24 Lysis1.seek 300uL @10:46:33.51 Lysis1.seek :empty @10:47:03.74 Lysis buffer delivered @13.3psi @10:47:03.76 Polling Can every 2:00 @10:47:04.40 PuckHeater.configure :default #ambient 27.4C @10:47:04.52 PuckHeater.seek 79.5C @10:47:44.96 PuckHeater.hold 2:00,79.5C @10:49:45.09 Polling Can every 2:00 @10:49:45.65 PuckHeater.configure :default #ambient 27.5C @10:49:45.77 PuckHeater.hold 30 seconds,78.5C @10:50:16.00 Polling Can every 2:00 @10:50:16.56 PuckHeater.hold 30 seconds,78C @10:50:46.82 Polling Can every 2:00 @10:50:47.39 PuckHeater.hold 10:00,77.5C @10:57:48.64 -> exit @10:57:48.66 Session Ended @11:00:47.22 Lysis complete. Syringe pressure @16.6psi @11:00:47.26 Archive syringe pumping up to 5 strokes to reach 40psi @11:00:47.30 Archive stroke #1 of 5 @11:00:47.46 Archive.in :pressurize @11:00:47.62 Archive.seek :pressurized @11:01:00.16 16.4psi after pressurize @11:01:05.76 16.3psi after pressurize (stable) @11:01:05.79 Archive.in :compress @11:01:05.91 Archive.seek :empty @11:01:16.77 Reached 40.4 of 40.0psi after pumping 1647uL (0.95 strokes) @11:01:16.79 Archive.in :default @11:01:16.98 Archive.hold @11:01:17.17 delay 2 seconds @11:01:19.19 Archive.in :fast @11:01:19.31 Archive.seek :full @11:01:26.24 Lysis1.seek 138uL @11:01:40.18 delay 1 second @11:01:41.22 Archive.seek :full @11:01:41.26 Lysis1.seek :empty @11:01:55.30 Handoff.select :blocked @11:01:55.47 Initial air pressure @44.0psi @11:01:55.55 Lysis1.in :extract @11:01:56.30 Lysis1.seek 600uL,nil @11:02:01.28 Archive syringe pumping up to 6 strokes to reach 25psi @11:02:01.31 Archive stroke #1 of 6 @11:02:01.56 Archive.in :pressurize @11:02:01.69 Archive.seek :pressurized @11:02:01.88 Archive.in :default @11:02:01.95 Archive.hold @11:02:02.37 Initial 600uL lysate @14.1psi @11:02:02.39 Handoff.select :blocked @11:02:02.56 Initial air pressure @14.1psi @11:02:02.68 Lysis1.seek 825uL,nil @11:02:02.97 Archive syringe pumping up to 6 strokes to reach 17psi @11:02:03.00 Archive stroke #1 of 6 @11:02:03.30 Archive.in :pressurize @11:02:03.44 Archive.seek :pressurized @11:02:15.81 14.0psi after pressurize @11:02:21.43 14.1psi after pressurize (stable) @11:02:21.47 Archive.in :compress @11:02:21.61 Archive.seek :empty @11:02:28.77 Archive.in :default @11:02:28.84 Archive.hold @11:02:29.21 Recovered 825uL lysate @15.2psi @11:02:29.24 Air.in :halfSpeed @11:02:29.37 Air.seek :fullVent @11:02:31.68 Puck Top Vented, bottom @17.4psi @11:02:31.70 Handoff.select :waste @11:02:31.89 delay 5 seconds @11:02:37.07 Handoff open to waste, bottom @0.4psi @11:02:37.13 Air.in :default @11:02:37.32 Air.seek :evac @11:02:38.77 Lysis2.in :DAmix @11:02:38.95 Lysis1.in :DAmix @11:02:39.67 Lysis2.seek 700uL @11:02:39.71 Lysis1.seek 575uL @11:03:00.73 Session Begun @11:03:02.52 -> exit @11:03:02.55 Session Ended @11:03:13.01 Session Begun @11:03:14.84 delay 5 seconds @11:03:19.86 Handoff.select SPR @11:03:20.04 SPR.command "start 2 14" @11:03:21.28 -> exit @11:03:21.31 Session Ended @11:03:21.72 SPR stabilized @11:03:21.94 delay 10 seconds @11:03:31.96 SPR.command "start 2 0" @11:03:35.09 Waiting up to 11:00 for SPR to enter state 1 @11:05:33.18 SPR.command "hold_on" @11:05:34.57 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:05:35.39 Lysis2.seek 400uL @11:05:35.46 Lysis1.seek 275uL @11:06:17.56 delay 15 seconds @11:06:32.59 SPR.command "hold_off" @11:06:35.22 Waiting up to 38:00 for SPR to enter state 7 @11:07:19.81 Session Ended @11:09:25.30 Session Begun @11:09:29.61 -> showlog 10 @11:20:55.40 SPRsummary:11.7RIU,4e+09RIU,113RIU,156RIU,none,114ng/L,174ng/L after 2835s @11:32:25.84 SPR analysis completed @11:32:32.53 Ended log spr23aug1310h.spr.bz2 @11:32:32.56 SPR.command "stats_off" @11:32:33.10 Handoff.select :close @11:32:33.31 Gate.power :heat,:OFF @11:32:33.34 Gate.power :cartridge,:OFF @11:32:33.49 PinPort.in :unlocking @11:32:33.60 PinPort.seek :clear @11:32:35.56 Carousel.in :default @11:32:35.74 Carousel.home.seek 24 @11:32:36.14 Plunge.seek :locked @11:32:37.13 Archive.in :unlocking @11:32:37.36 Archive.seek :clear @11:32:37.44 Air.in :unlocking @11:32:37.71 Air.seek :clear @11:32:37.84 Lysis1.in :unlocking @11:32:38.05 Lysis1.seek :clear @11:32:38.15 Lysis2.in :unlocking @11:32:38.31 Lysis2.seek :clear @11:32:42.22 Gate.power :motor,:OFF @11:32:42.24 Gate.power :core,:OFF @11:32:42.31 Gate.power :analytic1,:OFF @11:32:42.34 Gate.power :analytic2,:OFF @11:32:42.37 Gate.power :main,:OFF @11:32:42.43 Safely stopped and ready to power off @11:33:30.32 -> ESP.ready!; SPR.start; delay 2; SPRcommand "start 3 0"; delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @11:33:30.40 Gate.power :sampler,:OFF,limit to 3500mA @11:33:30.43 Gate.power :main,:ON,limit to 5000mA @11:33:30.47 Gate.power :analytic1,:ON,limit to 5000mA @11:33:30.51 Gate.power :analytic2,:ON,limit to 5000mA @11:33:30.65 Powering core bus @11:33:33.04 SPR Module 1, FW Rev. B, FPGA Rev. C @11:33:33.70 Archive.configure :default @11:33:33.83 Air.configure :default @11:33:33.94 Lysis1.configure :default @11:33:34.04 Lysis2.configure :default @11:33:34.19 Plunge.configure :default @11:33:34.33 SamplePump.configure :default @11:33:34.47 Toroid.configure :default @11:33:34.61 Carousel.configure :default @11:33:34.75 Pressure.configure :default @11:33:34.88 ExtraPump.configure :default @11:33:35.00 PinPort.configure :default @11:33:35.13 All elf controllers running firmware version 4.84 @11:33:35.16 Gate.power :motor,:ON,limit to 5000mA @11:33:35.45 Archive.home.jog 0 @11:33:36.27 PinPort.home.jog 0 @11:33:36.32 Toroid.home.jog 0 @11:33:36.37 Plunge.home.jog 0 @11:33:36.44 Lysis1.home.jog 0 @11:33:36.51 Lysis2.home.jog 0 @11:33:36.56 Air.home.jog 0 @11:33:36.64 Carousel.home.jog 0 @11:33:37.53 Archive.in :homing @11:33:37.93 Archive.jog 1387 @11:33:38.01 PinPort.in :homing @11:33:38.06 Plunge.in :homing @11:33:38.12 Lysis1.in :homing @11:33:38.23 Toroid.in :homing @11:33:38.27 Air.in :homing @11:33:38.33 Lysis2.in :homing @11:33:38.74 Plunge.jog 1578 @11:33:38.78 PinPort.jog -107 @11:33:38.86 Lysis1.jog 1388 @11:33:38.94 Toroid.jog 584 @11:33:39.10 Lysis2.jog 1392 @11:33:39.15 Air.jog 1370 @11:33:39.58 PinPort.setRawPosition! 0 @11:33:39.68 PinPort.seek :home @11:33:39.82 Archive.setRawPosition! 50 @11:33:39.93 Archive.seek :home @11:33:40.06 PinPort.in :default @11:33:40.24 Archive.in :default @11:33:40.38 Plunge.setRawPosition! 50 @11:33:40.47 Plunge.seek :home @11:33:40.64 Lysis1.setRawPosition! 50 @11:33:40.68 Air.setRawPosition! 50 @11:33:40.73 Lysis2.setRawPosition! 50 @11:33:40.93 Lysis1.seek :home @11:33:41.03 Lysis2.seek :home @11:33:41.15 Air.seek :home @11:33:41.43 Toroid.setRawPosition! 0 @11:33:41.47 Plunge.in :default @11:33:41.53 Lysis1.in :default @11:33:41.75 Toroid.seek :home @11:33:41.95 Toroid.in :default @11:33:41.99 Lysis2.in :default @11:33:42.03 Air.in :default @11:33:42.55 Toroid.seek :clear @11:33:44.21 Carousel.in :default @11:33:44.39 Carousel.home.seek 24 @11:33:45.81 SPR.command "read_sequences" @11:33:47.02 SPR.command "stats_on" @11:33:47.12 delay 2 seconds @11:33:47.91 SPR.start /var/log/esp/spr23aug1311h.spr.bz2 @11:33:49.15 NoMethodError in errera -- undefined method `SPRcommand' for CmdServer::Client:Class @11:34:17.03 -> SPR.command "start 2 14" @11:34:17.06 SPR.command "start 2 14" @11:34:26.92 -> SPR.stop @11:34:27.34 Ended log spr23aug1311h.spr.bz2 @11:34:27.36 SPR.command "stats_off" @11:34:42.96 -> delay 10 @11:34:43.00 delay 10 seconds @11:35:18.15 -> Cmd.stop @11:35:18.40 Carousel.in :default @11:35:18.57 Carousel.home.seek 24 @11:35:18.97 Lysis1.in :fast @11:35:19.11 Lysis1.seek :locked @11:35:21.68 Plunge.seek :locked @11:35:23.10 Lysis1.in :unlocking @11:35:23.31 Lysis1.seek :clear @11:35:24.84 Gate.power :motor,:OFF @11:35:24.87 Gate.power :core,:OFF @11:35:24.92 Gate.power :analytic1,:OFF @11:35:24.96 Gate.power :analytic2,:OFF @11:35:25.05 Gate.power :main,:OFF @11:35:25.10 Safely stopped and ready to power off :STOPPED @11:37:04.24 -> ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @11:37:04.31 Gate.power :sampler,:OFF,limit to 3500mA @11:37:04.35 Gate.power :main,:ON,limit to 5000mA @11:37:04.41 Gate.power :analytic1,:ON,limit to 5000mA @11:37:04.43 Gate.power :analytic2,:ON,limit to 5000mA @11:37:04.56 Powering core bus @11:37:06.90 SPR Module 1, FW Rev. B, FPGA Rev. C @11:37:07.35 Archive.configure :default @11:37:07.49 Air.configure :default @11:37:07.60 Lysis1.configure :default @11:37:07.71 Lysis2.configure :default @11:37:07.85 Plunge.configure :default @11:37:07.99 SamplePump.configure :default @11:37:08.12 Toroid.configure :default @11:37:08.26 Carousel.configure :default @11:37:08.41 Pressure.configure :default @11:37:08.55 ExtraPump.configure :default @11:37:08.66 PinPort.configure :default @11:37:08.77 All elf controllers running firmware version 4.84 @11:37:08.82 Gate.power :motor,:ON,limit to 5000mA @11:37:09.29 Archive.home.jog 0 @11:37:10.01 PinPort.home.jog 0 @11:37:10.06 Toroid.home.jog 0 @11:37:10.13 Plunge.home.jog 0 @11:37:10.17 Lysis1.home.jog 0 @11:37:10.25 Air.home.jog 0 @11:37:10.32 Carousel.home.jog 0 @11:37:10.40 Lysis2.home.jog 0 @11:37:11.91 Archive.in :homing @11:37:12.29 Archive.jog 1387 @11:37:12.37 PinPort.in :homing @11:37:12.42 Plunge.in :homing @11:37:12.50 Lysis1.in :homing @11:37:12.58 Lysis2.in :homing @11:37:12.64 Air.in :homing @11:37:12.70 Toroid.in :homing @11:37:13.12 PinPort.jog -107 @11:37:13.21 Plunge.jog 1568 @11:37:13.27 Lysis1.jog 1389 @11:37:13.35 Lysis2.jog 1391 @11:37:13.40 Air.jog 1365 @11:37:13.49 Toroid.jog 576 @11:37:13.95 PinPort.setRawPosition! 0 @11:37:14.05 PinPort.seek :home @11:37:14.20 Archive.setRawPosition! 50 @11:37:14.23 PinPort.in :default @11:37:14.39 Archive.seek :home @11:37:14.73 Archive.in :default @11:37:14.88 Lysis1.setRawPosition! 50 @11:37:14.98 Lysis1.seek :home @11:37:15.16 Plunge.setRawPosition! 50 @11:37:15.19 Lysis2.setRawPosition! 50 @11:37:15.26 Air.setRawPosition! 50 @11:37:15.46 Plunge.seek :home @11:37:15.50 Lysis2.seek :home @11:37:15.56 Air.seek :home @11:37:15.89 Lysis1.in :default @11:37:15.92 Toroid.setRawPosition! 0 @11:37:16.11 Toroid.seek :home @11:37:16.29 Plunge.in :default @11:37:16.34 Lysis2.in :default @11:37:16.39 Toroid.in :default @11:37:16.46 Air.in :default @11:37:17.03 Toroid.seek :clear @11:37:18.69 Carousel.in :default @11:37:18.87 Carousel.home.seek 24 @11:37:20.32 SPR.command "read_sequences" @11:37:20.92 SPR.command "stats_on" @11:37:21.07 delay 2 seconds @11:37:21.86 SPR.start /var/log/esp/spr23aug1311h#2.spr.bz2 @11:37:23.09 SPR.command "start 3 0" @11:37:24.30 delay 15:00 @11:52:24.32 SPR.command "start 2 14" @11:52:31.21 Ended log spr23aug1311h#2.spr.bz2 @11:52:31.24 SPR.command "stats_off" @11:52:31.77 delay 10 seconds @11:52:41.96 Carousel.in :default @11:52:42.13 Carousel.home.seek 24 @11:52:42.52 Lysis1.in :fast @11:52:42.63 Lysis1.seek :locked @11:52:45.20 Plunge.seek :locked @11:52:46.64 Lysis1.in :unlocking @11:52:46.86 Lysis1.seek :clear @11:52:48.40 Gate.power :motor,:OFF @11:52:48.43 Gate.power :core,:OFF @11:52:48.47 Gate.power :analytic1,:OFF @11:52:48.55 Gate.power :analytic2,:OFF @11:52:48.58 Gate.power :main,:OFF @11:52:48.62 Safely stopped and ready to power off :STOPPED @11:56:12.26 -> start {fire :archiveHiBiomass_bac; :microSPR_phyto} # @11:56:12.53 Gate.power :sampler,:OFF,limit to 3500mA @11:56:12.56 Gate.power :main,:ON,limit to 5000mA @11:56:12.61 Gate.power :analytic1,:ON,limit to 5000mA @11:56:12.66 Gate.power :analytic2,:ON,limit to 5000mA @11:56:12.80 Powering core bus @11:56:15.14 SPR Module 1, FW Rev. B, FPGA Rev. C @11:56:15.59 Archive.configure :default @11:56:15.74 Air.configure :default @11:56:15.85 Lysis1.configure :default @11:56:15.97 Lysis2.configure :default @11:56:16.10 Plunge.configure :default @11:56:16.25 SamplePump.configure :default @11:56:16.39 Toroid.configure :default @11:56:16.53 Carousel.configure :default @11:56:16.67 Pressure.configure :default @11:56:16.83 ExtraPump.configure :default @11:56:16.94 PinPort.configure :default @11:56:17.10 All elf controllers running firmware version 4.84 @11:56:17.13 Gate.power :motor,:ON,limit to 5000mA @11:56:17.52 Archive.home.jog 0 @11:56:18.79 PinPort.home.jog 0 @11:56:18.83 Toroid.home.jog 0 @11:56:18.87 Plunge.home.jog 0 @11:56:18.96 Lysis1.home.jog 0 @11:56:19.06 Lysis2.home.jog 0 @11:56:19.12 Air.home.jog 0 @11:56:19.17 Carousel.home.jog 0 @11:56:20.06 Archive.in :homing @11:56:20.50 Archive.jog 1386 @11:56:20.57 PinPort.in :homing @11:56:20.64 Plunge.in :homing @11:56:20.73 Lysis1.in :homing @11:56:20.81 Toroid.in :homing @11:56:20.87 Air.in :homing @11:56:20.93 Lysis2.in :homing @11:56:21.38 PinPort.jog -108 @11:56:21.43 Plunge.jog 1565 @11:56:21.50 Lysis1.jog 1388 @11:56:21.57 Air.jog 1363 @11:56:21.65 Toroid.jog 574 @11:56:21.70 Lysis2.jog 1393 @11:56:22.19 PinPort.setRawPosition! 0 @11:56:22.29 PinPort.seek :home @11:56:22.48 Archive.setRawPosition! 50 @11:56:22.51 PinPort.in :default @11:56:22.68 Archive.seek :home @11:56:23.07 Archive.in :default @11:56:23.11 Lysis1.setRawPosition! 50 @11:56:23.32 Lysis1.seek :home @11:56:23.47 Plunge.setRawPosition! 50 @11:56:23.53 Lysis2.setRawPosition! 50 @11:56:23.58 Air.setRawPosition! 50 @11:56:23.79 Lysis2.seek :home @11:56:23.87 Plunge.seek :home @11:56:23.92 Air.seek :home @11:56:24.20 Toroid.setRawPosition! 0 @11:56:24.26 Lysis1.in :default @11:56:24.45 Toroid.seek :home @11:56:24.65 Toroid.in :default @11:56:24.70 Plunge.in :default @11:56:24.77 Air.in :default @11:56:24.82 Lysis2.in :default @11:56:25.44 Toroid.seek :clear @11:56:27.11 Carousel.in :default @11:56:27.29 Carousel.home.seek 24 @11:56:27.65 Selecting Cartridge 23 @11:56:28.01 Carousel.seek 23 @11:56:28.60 Lysis1.in :fast @11:56:28.72 Lysis1.seek :locked @11:56:31.64 Plunge.seek :locked @11:56:31.86 Air.in :fast @11:56:31.98 Air.seek :locked @11:56:33.61 Carousel.reconfigure :locked @11:56:33.84 Powering cartridge bus @11:56:36.50 Gate.setIR 1750,5000,0,6 @11:56:36.63 Gate.setIR 0,5000,0,6 @11:56:36.76 Gate.power :cartridge,:OFF @11:56:37.35 Mounted archive Cartridge @11:56:37.74 Preparing to filter cartridge 23 @11:56:38.04 Gate.power :sampler,:ON @11:56:38.19 Priming sample loop w/150ml, bypass w/50ml @11:56:38.21 SamplePump.setPosition! 0ml @11:56:38.32 Bypass.open @11:56:39.87 Intake.open @11:56:40.66 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:56:43.26 Vacuum tare is 56% of full scale @11:56:43.48 Pressure.in :default @11:56:43.59 Pressure.hold @11:56:43.87 Waiting for external sampler to finish priming... @11:56:43.89 delayUntil "12:00:08.06" @12:00:08.11 SamplePump.in :prime @12:00:08.24 SamplePump.seek 50ml,2:53 @12:01:09.28 Pressure.stop @12:01:09.38 SamplePump.setPosition! 0ml @12:01:09.47 Exhaust.open @12:01:10.95 Bypass.close @12:01:12.60 Pressure.in :default @12:01:12.71 Pressure.hold @12:01:13.11 SamplePump.in :prime @12:01:13.25 SamplePump.seek 150ml,7:39 @12:04:15.66 Pressure.stop @12:04:15.79 Bypass.open @12:04:16.55 Intake.close @12:04:17.36 Toroid.in :hold @12:04:17.48 Toroid.seek :crack @12:04:19.06 Toroid.seek :cartridge @12:04:20.33 Toroid.in :default @12:04:20.45 Toroid.seek :clear @12:04:22.52 Pressurizing cartridge to 26psi above 13.4psia @12:04:22.54 SamplePump.setPosition! 0ml @12:04:22.65 Intake.open @12:04:23.33 Bypass.close @12:04:24.10 Exhaust.close @12:04:25.00 Pressure.in :default @12:04:25.14 Pressure.hold @12:04:25.44 SamplePump.in :charge @12:04:25.56 SamplePump.seek 2.3ml,42 seconds @12:04:33.94 Pressure.stop @12:04:34.06 Intake.close @12:04:34.84 Pressurized cartridge with 0.312ml to 26.3psi @12:04:34.86 Isolated @34.4psia (21.0psi) @12:04:34.92 Verifying cartridge holds at least 0.65..0.85 of 34.4psia @12:04:35.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:04:41.16 Cartridge pressure fell 8% to 31.6psia @12:04:41.32 Sampling 1000.0ml @12:04:41.34 SamplePump.setPosition! 0ml @12:04:41.46 Exhaust.open @12:04:42.24 Intake.open @12:04:43.13 Pressure.in :default @12:04:43.24 Pressure.hold @12:04:43.43 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:04:43.68 SamplePump.in :sample @12:04:43.81 SamplePump.seek 1000ml,1:23:51 @12:04:46.81 Cartridge 23 filtering 1/1000ml at 0.59ml/s with 31.7psi vacuum @12:05:46.81 Cartridge 23 filtering 33/1000ml at 0.52ml/s with 20.3psi vacuum @12:06:46.81 Cartridge 23 filtering 63/1000ml at 0.52ml/s with 24.4psi vacuum @12:07:46.81 Cartridge 23 filtering 91/1000ml at 0.49ml/s with 28.6psi vacuum @12:08:46.81 Cartridge 23 filtering 118/1000ml at 0.37ml/s with 28.4psi vacuum @12:09:44.92 SamplePump.stop @12:09:45.16 Intake.close @12:09:45.21 Exhaust.close @12:09:45.58 Toroid.in :hold @12:09:45.71 Toroid.seek :bypass @12:09:46.81 Cartridge 23 filtering 144/1000ml at 0.00ml/s with -1.1psi vacuum @12:09:47.41 Toroid.in :default @12:09:47.53 Toroid.seek :clear @12:09:49.20 Pressure.stop @12:09:49.77 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 0.0psi vacuum after 143.5ml @12:09:49.79 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 143.53ml" @12:09:52.64 Gate.power :sampler,:OFF @12:19:29.70 -> Power.sampler :on @12:19:29.73 Gate.power :sampler,:ON true @12:20:12.90 -> BACsampler.prime @12:20:13.08 Priming sample loop w/150ml, bypass w/50ml @12:20:13.10 SamplePump.setPosition! 0ml @12:20:13.21 Bypass.open @12:20:14.88 Intake.open @12:20:16.41 Pressure.in :default @12:20:16.52 Pressure.hold @12:20:16.80 Waiting for external sampler to finish priming... @12:20:16.82 delayUntil "12:22:59.76" @12:20:52.65 SamplePump.stop @12:20:52.82 Intake.close @12:20:52.88 Bypass.close @12:20:53.80 Pressure.stop @12:20:53.91 UserAbort in brent -- User Abort!! @12:21:03.55 -> Toroid Toroid Valve (TV) clear @12:21:15.72 -> Toroid.bypass @12:21:15.89 Toroid.in :hold @12:21:15.99 Toroid.seek :bypass @12:21:17.68 Toroid.in :default @12:21:17.80 Toroid.seek :clear Toroid Valve (TV) clear @12:21:21.69 -> BACsampler.prime @12:21:21.84 Priming sample loop w/150ml, bypass w/50ml @12:21:21.86 SamplePump.setPosition! 0ml @12:21:21.96 Bypass.open @12:21:23.57 Intake.open @12:21:25.13 Pressure.in :default @12:21:25.24 Pressure.hold @12:21:25.51 Waiting for external sampler to finish priming... @12:21:25.53 delayUntil "12:22:59.76" @12:22:59.82 SamplePump.in :prime @12:22:59.94 SamplePump.seek 50ml,2:53 @12:24:00.95 Pressure.stop @12:24:01.08 SamplePump.setPosition! 0ml @12:24:01.17 Exhaust.open @12:24:03.24 Bypass.close @12:24:04.92 Pressure.in :default @12:24:05.06 Pressure.hold @12:24:05.42 SamplePump.in :prime @12:24:05.54 SamplePump.seek 150ml,7:39 @12:26:42.93 Session Begun @12:26:45.47 -> SP Sample Pump (SP) at 131.68ml @12:27:06.06 -> hidelog Log display suppressed @12:27:07.99 Pressure.stop BACsampler @12:27:18.69 -> Power.sampler true @12:27:34.35 -> progress >>> Thread[:MAIN] is sleeping <<< @10:25:06 Waiting for espclients... >>> Thread["brent"] is running <<< @12:20:13 Priming sample loop w/150ml, bypass w/50ml @12:21:21 Priming sample loop w/150ml, bypass w/50ml >>> Thread["detached from errera"] is sleeping <<< @11:56:27 Selecting Cartridge 23 >>> Thread[:FILTERING] is suspended <<< @11:56:37 Preparing to filter cartridge 23 @11:56:38 Priming sample loop w/150ml, bypass w/50ml @11:56:40 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:04:22 Pressurizing cartridge to 26psi above 13.4psia @12:04:28 Sample Pump (SP) pressureOutOfBounds at 0.31ml @12:04:34 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:04:41 Sampling 1000.0ml @12:09:36 Sample Pump (SP) pressureOutOfBounds at 143.53ml @12:28:06.43 -> start {Cmd.resume} @12:28:06.61 Resuming Thread (suspended since 11:56:37) # @12:28:06.67 Preparing to filter cartridge 23 @12:28:07.12 Toroid.in :hold @12:28:07.24 Toroid.seek :cartridge @12:28:09.11 Toroid.in :default @12:28:09.21 Toroid.seek :clear @12:28:11.06 Sampling 856.5ml @12:28:11.09 SamplePump.setPosition! 0ml @12:28:11.39 Pressure.in :default @12:28:11.50 Pressure.hold @12:28:11.70 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:28:11.93 SamplePump.in :sample @12:28:12.06 SamplePump.seek 856.5ml,1:11:53 @12:28:15.09 Cartridge 23 filtering 1/856ml at 0.40ml/s with 32.3psi vacuum @12:29:15.09 Cartridge 23 filtering 25/856ml at 0.38ml/s with 13.6psi vacuum 2.7m deep (18.8psia) @12:30:15.10 Cartridge 23 filtering 46/856ml at 0.28ml/s with 26.6psi vacuum @12:30:47.72 SamplePump.stop @12:30:47.84 Intake.close @12:30:47.90 Exhaust.close @12:30:48.26 Toroid.in :hold @12:30:48.38 Toroid.seek :bypass @12:30:50.08 Toroid.in :default @12:30:50.20 Toroid.seek :clear @12:30:51.87 Pressure.stop @12:30:52.42 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 0.0psi vacuum after 59.1ml @12:30:52.44 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 59.05ml" @12:30:55.29 Gate.power :sampler,:OFF @12:31:35.63 -> progress >>> Thread[:MAIN] is sleeping <<< @10:25:06 Waiting for espclients... >>> Thread["brent"] is running <<< @12:20:13 Priming sample loop w/150ml, bypass w/50ml @12:21:21 Priming sample loop w/150ml, bypass w/50ml >>> Thread["detached from errera"] is sleeping <<< @11:56:27 Selecting Cartridge 23 >>> Thread[:FILTERING] is suspended <<< @11:56:37 Preparing to filter cartridge 23 @11:56:38 Priming sample loop w/150ml, bypass w/50ml @11:56:40 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:04:22 Pressurizing cartridge to 26psi above 13.4psia @12:04:28 Sample Pump (SP) pressureOutOfBounds at 0.31ml @12:04:34 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:04:41 Sampling 1000.0ml @12:09:36 Sample Pump (SP) pressureOutOfBounds at 143.53ml @12:28:11 Sampling 856.5ml @12:30:45 Sample Pump (SP) pressureOutOfBounds at 59.05ml @12:32:13.07 -> Cart.prime 50,50 @12:32:13.12 ArgumentError in brent -- wrong number of arguments (3 for 2) @12:32:37.71 -> BACsampler.prime 50,50 @12:32:37.85 Priming sample loop w/50ml, bypass w/50ml @12:32:37.87 SamplePump.setPosition! 0ml @12:32:37.98 Bypass.open @12:32:40.23 Intake.open @12:32:41.80 Pressure.in :default @12:32:41.91 Pressure.hold @12:32:42.18 Gate.power :sampler,:ON @12:32:42.21 Waiting for external sampler to finish priming... @12:32:42.26 delayUntil "12:36:12.20" @12:35:18.59 -> BACsampler.goalVolume 1000 @12:35:26.67 -> Cart.sampler.goalVolume 1000 @12:36:12.26 SamplePump.in :prime @12:36:12.39 SamplePump.seek 50ml,2:53 @12:37:13.38 Pressure.stop @12:37:13.48 SamplePump.setPosition! 0ml @12:37:13.56 Exhaust.open @12:37:15.07 Bypass.close @12:37:16.69 Pressure.in :default @12:37:16.79 Pressure.hold @12:37:17.19 SamplePump.in :prime @12:37:17.31 SamplePump.seek 50ml,2:53 @12:37:19.31 -> Crt @12:37:19.34 NameError in brent2 -- uninitialized constant Crt @12:37:21.15 -> Cart { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 202.6} @12:38:17.17 SamplePump.stop @12:38:17.27 Intake.close @12:38:17.33 Exhaust.close @12:38:18.25 Pressure.stop @12:38:18.37 Cartridge::Sampler::Leaky in brent -- Serious Leak or Stall -- 0.82ml/s made only -0.3psi vacuum after 49.1ml @12:39:32.75 -> progress >>> Thread[:MAIN] is sleeping <<< @10:25:06 Waiting for espclients... >>> Thread["brent"] is running <<< @12:20:13 Priming sample loop w/150ml, bypass w/50ml @12:21:21 Priming sample loop w/150ml, bypass w/50ml @12:32:37 Priming sample loop w/50ml, bypass w/50ml @12:38:11 Sample Pump (SP) pressureOutOfBounds at 49.08ml >>> Thread["detached from errera"] is sleeping <<< @11:56:27 Selecting Cartridge 23 >>> Thread[:FILTERING] is suspended <<< @11:56:37 Preparing to filter cartridge 23 @11:56:38 Priming sample loop w/150ml, bypass w/50ml @11:56:40 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:04:22 Pressurizing cartridge to 26psi above 13.4psia @12:04:28 Sample Pump (SP) pressureOutOfBounds at 0.31ml @12:04:34 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:04:41 Sampling 1000.0ml @12:09:36 Sample Pump (SP) pressureOutOfBounds at 143.53ml @12:28:11 Sampling 856.5ml @12:30:45 Sample Pump (SP) pressureOutOfBounds at 59.05ml @12:39:56.43 -> start {Cmd.resume} @12:39:56.62 Resuming Thread (suspended since 11:56:37) @12:39:56.65 # Preparing to filter cartridge 23 @12:39:57.13 Toroid.in :hold @12:39:57.25 Toroid.seek :cartridge @12:39:59.12 Toroid.in :default @12:39:59.24 Toroid.seek :clear @12:40:01.12 Sampling 797.4ml @12:40:01.16 SamplePump.setPosition! 0ml @12:40:01.32 Exhaust.open @12:40:02.11 Intake.open @12:40:03.05 Pressure.in :default @12:40:03.17 Pressure.hold @12:40:03.36 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:40:03.61 SamplePump.in :sample @12:40:03.75 SamplePump.seek 797.4ml,1:06:58 @12:40:06.74 Cartridge 23 filtering 1/797ml at 0.39ml/s with 27.2psi vacuum @12:40:07.95 -> Intake.close @12:40:07.99 Intake.close Intake is closed @12:40:09.60 Flow estimate reduced to 0.66ml/s @12:40:09.68 Waiting up to 4:40 for vacuum to reduce from 134.8 to 18.0psi @12:40:13.09 Relieved excess vacuum in 3.5 seconds @12:40:13.13 SamplePump.seek 797.4ml,1:06:49 @12:40:14.54 Flow estimate reduced to 0.53ml/s @12:40:14.64 Waiting up to 2:46 for vacuum to reduce from 89.2 to 18.0psi @12:40:17.75 Relieved excess vacuum in 3.2 seconds @12:40:17.81 SamplePump.seek 797.4ml,1:06:48 @12:40:19.19 Flow estimate reduced to 0.42ml/s @12:40:19.28 Waiting up to 3:05 for vacuum to reduce from 96.8 to 18.0psi @12:40:28.13 Relieved excess vacuum in 8.9 seconds @12:40:28.21 SamplePump.seek 797.4ml,1:06:47 @12:40:29.60 Flow estimate reduced to 0.34ml/s @12:40:29.70 Waiting up to 3:03 for vacuum to reduce from 96.0 to 18.0psi @12:40:38.02 Relieved excess vacuum in 8.4 seconds @12:40:38.08 SamplePump.seek 797.4ml,1:06:46 @12:40:39.49 Flow estimate reduced to 0.27ml/s @12:40:39.58 Waiting up to 3:15 for vacuum to reduce from 100.9 to 18.0psi @12:40:43.43 Relieved excess vacuum in 3.9 seconds @12:40:43.51 SamplePump.seek 797.4ml,1:06:45 @12:40:44.85 Flow estimate reduced to 0.22ml/s @12:40:44.91 Waiting up to 2:47 for vacuum to reduce from 89.6 to 18.0psi @12:40:49.79 Relieved excess vacuum in 4.9 seconds @12:40:49.88 SamplePump.seek 797.4ml,1:06:44 @12:40:51.23 Flow estimate reduced to 0.17ml/s @12:40:51.30 Exhaust.close @12:40:51.57 Toroid.in :hold @12:40:51.70 Toroid.seek :bypass @12:40:53.40 Toroid.in :default @12:40:53.53 Toroid.seek :clear @12:40:55.76 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @12:40:55.80 Pressure.stop @12:40:56.12 Sampled 3.0ml @12:40:56.85 Plunge.seek :reagent @12:40:57.71 Sample loop closed. Ready to Deliver Reagents @12:40:57.75 Archive.seek :sealed @12:40:59.30 Gate.power :sampler,:OFF @12:41:01.75 Archive.seek :evac @12:41:15.84 delay 1:00 @12:42:15.88 Archive.seek :clear @12:42:33.42 RNAlater soak for 10:00 @12:52:33.46 Archive.seek :tVent @12:52:35.96 delay 1 second @12:52:37.00 Archive.seek :evac @12:52:52.59 delay 10 seconds @12:53:02.63 Archive.seek :tVent @12:53:18.18 delay 1 second @12:53:19.21 Archive.seek :evac @12:53:34.77 delay 10 seconds @12:53:44.81 Sample preserved and evacuated @12:53:45.18 Carousel.in :default @12:53:45.36 Carousel.home.seek 23 @12:53:45.78 Plunge.seek :locked @12:53:46.73 Archive.in :unlocking @12:53:46.91 Archive.seek :clear @12:53:47.09 Air.in :unlocking @12:53:47.31 Air.seek :clear @12:53:47.39 Lysis1.in :unlocking @12:53:47.63 Lysis1.seek :clear @12:53:51.53 Gate.power :motor,:OFF @12:53:51.56 Gate.power :core,:OFF @12:53:51.61 Gate.power :analytic1,:OFF @12:53:51.65 Gate.power :analytic2,:OFF @12:53:51.70 Gate.power :main,:OFF @12:53:51.73 Safely stopped and ready to power off @12:54:15.88 -> eit :eit @12:54:17.39 -> exit @12:54:17.41 Session Ended @12:54:19.25 Session Begun @12:54:55.64 -> exit @12:54:55.67 Session Ended @12:56:42.37 -> start {fire :microSPR_phyto} # @12:56:42.65 Gate.power :sampler,:OFF,limit to 3500mA @12:56:42.69 Gate.power :main,:ON,limit to 5000mA @12:56:42.73 Gate.power :analytic1,:ON,limit to 5000mA @12:56:42.78 Gate.power :analytic2,:ON,limit to 5000mA @12:56:42.91 Powering core bus @12:56:45.26 SPR Module 1, FW Rev. B, FPGA Rev. C @12:56:45.70 Archive.configure :default @12:56:45.84 Air.configure :default @12:56:45.95 Lysis1.configure :default @12:56:46.06 Lysis2.configure :default @12:56:46.20 Plunge.configure :default @12:56:46.92 SamplePump.configure :default @12:56:47.09 Toroid.configure :default @12:56:47.27 Carousel.configure :default @12:56:47.41 Pressure.configure :default @12:56:47.56 ExtraPump.configure :default @12:56:47.68 PinPort.configure :default @12:56:47.80 All elf controllers running firmware version 4.84 @12:56:47.83 Gate.power :motor,:ON,limit to 5000mA @12:56:48.21 Archive.home.jog 0 @12:56:48.96 PinPort.home.jog 0 @12:56:49.06 Toroid.home.jog 0 @12:56:49.12 Plunge.home.jog 0 @12:56:49.18 Lysis1.home.jog 0 @12:56:49.25 Air.home.jog 0 @12:56:49.33 Carousel.home.jog 0 @12:56:49.38 Lysis2.home.jog 0 @12:56:50.43 Archive.in :homing @12:56:50.71 Archive.jog 1390 @12:56:50.79 Toroid.in :homing @12:56:50.86 Lysis2.in :homing @12:56:50.91 Air.in :homing @12:56:51.03 PinPort.in :homing @12:56:51.12 Lysis1.in :homing @12:56:51.22 Plunge.in :homing @12:56:51.55 Toroid.jog 581 @12:56:51.61 Lysis2.jog 1397 @12:56:51.74 Air.jog 1362 @12:56:51.86 PinPort.jog -108 @12:56:51.93 Plunge.jog 1573 @12:56:52.00 Lysis1.jog 1387 @12:56:52.49 PinPort.setRawPosition! 0 @12:56:52.52 Archive.setRawPosition! 50 @12:56:52.71 Archive.seek :home @12:56:52.74 PinPort.seek :home @12:56:53.05 PinPort.in :default @12:56:53.19 Archive.in :default @12:56:53.35 Air.setRawPosition! 50 @12:56:53.39 Lysis2.setRawPosition! 50 @12:56:53.56 Air.seek :home @12:56:53.67 Lysis2.seek :home @12:56:53.84 Plunge.setRawPosition! 50 @12:56:53.91 Lysis1.setRawPosition! 50 @12:56:54.00 Toroid.setRawPosition! 0 @12:56:54.15 Plunge.seek :home @12:56:54.26 Lysis1.seek :home @12:56:54.35 Toroid.seek :home @12:56:54.70 Plunge.in :default @12:56:54.75 Lysis2.in :default @12:56:54.81 Toroid.in :default @12:56:54.87 Air.in :default @12:56:55.23 Lysis1.in :default @12:56:55.61 Toroid.seek :clear @12:56:57.28 Carousel.in :default @12:56:57.95 Carousel.home.seek 23 @12:56:58.26 Selecting Cartridge 22 @12:56:58.63 Carousel.seek 22 @12:56:59.24 Lysis1.in :fast @12:56:59.35 Lysis1.seek :locked @12:57:02.02 Plunge.seek :locked @12:57:02.25 Air.in :fast @12:57:02.35 Air.seek :locked @12:57:03.98 Carousel.reconfigure :locked @12:57:04.20 Powering cartridge bus @12:57:06.87 Gate.setIR 1750,5000,0,6 @12:57:07.01 Gate.setIR 0,5000,0,6 @12:57:07.14 PuckHeater.configure :default #ambient 27.5C @12:57:07.26 Gate.power :heat,:ON,limit to 5000mA @12:57:07.96 Mounted lysis Cartridge board#Q05090 @12:57:08.03 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:57:08.42 Preparing to filter cartridge 22 @12:57:08.71 Gate.power :sampler,:ON @12:57:08.86 Priming sample loop w/150ml, bypass w/50ml @12:57:08.89 SamplePump.setPosition! 0ml @12:57:09.03 Bypass.open @12:57:10.58 Intake.open @12:57:11.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:57:14.01 Vacuum tare is 56% of full scale @12:57:14.22 Pressure.in :default @12:57:14.33 Pressure.hold @12:57:14.62 Waiting for external sampler to finish priming... @12:57:14.64 delayUntil "13:00:38.74" @12:59:10.55 -> Cmd.stop @12:59:10.59 Pressure.stop @12:59:10.66 Intake.close @12:59:10.76 Bypass.close @12:59:12.11 Gate.power :heat,:OFF @12:59:12.13 Gate.power :cartridge,:OFF @12:59:12.86 Carousel.in :default @12:59:13.15 Cartridge::Error in detached from errera -- Cannot process dry cartridge 22 @12:59:13.20 Carousel.home.seek 22 @12:59:13.23 Waiting to Resume from Checkpoint "Selecting Cartridge 22" @12:59:13.66 Plunge.seek :locked @12:59:13.98 Air.in :unlocking @12:59:14.17 Air.seek :clear @12:59:14.32 Lysis1.in :unlocking @12:59:14.52 Lysis1.seek :clear @12:59:14.74 Gate.power :sampler,:OFF @12:59:16.06 Gate.power :motor,:OFF @12:59:16.09 Gate.power :core,:OFF @12:59:16.14 Gate.power :analytic1,:OFF @12:59:16.18 Gate.power :analytic2,:OFF @12:59:16.23 Gate.power :main,:OFF @12:59:16.28 Safely stopped and ready to power off :STOPPED @12:59:50.71 Session Begun @13:00:07.32 -> list method :startDoublet require 'mail' def startDoublet start {fire :archiveHiBiomass_bac, :microSPR_phyto} end class Sampler unless @@default.key? :recoveryVolume #circulate water before starting to prime internal lines assign :PumpPrimeDelay, -3.5.minutes accelTweaks = { bypassVolume: 50, #override default bypass priming volume primeVolume: 150, #override default sample loop priming volume evacPSIrise: 30, #psi rise required during 2nd evac finalLysingPressure: 40, #pressure applied after cooking lysate (PSI) recoveryBubblePt: 16, #lysate recovery bubble point (PSI) recoveryPressure: 17..25, #pressure maintained during recovery (PSI) recoveryVolume: 600..825, #lysate recovered (uL) @13:01:00.28 -> slot {[32,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1310h.spr.bz2}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @13:01:17.32 -> require 'vacuumCart'3.times{refillsampleLines} BACsampler.prime 300,100 @13:01:17.36 SyntaxError in errera -- compile error errera:11: syntax error, unexpected tINTEGER, expecting $end require 'vacuumCart'3.times{refillsampleLines} BACs... ^ @13:02:55.08 -> require 'vacuumCart' true @13:03:13.80 -> 3.times[refillSampleLines} @13:03:13.85 SyntaxError in errera -- compile error errera:13: syntax error, unexpected '}', expecting ']' 3.times[refillSampleLines} ^ @13:03:24.04 -> #what are you trying to do? @13:03:47.32 -> 3.times{refillSampleLines} @13:03:47.35 Refilling sample & bypass lines with fluid. @13:03:47.48 Gate.power :sampler,:OFF,limit to 3500mA @13:03:47.51 Gate.power :main,:ON,limit to 5000mA @13:03:47.56 Gate.power :analytic1,:ON,limit to 5000mA @13:03:47.63 Gate.power :analytic2,:ON,limit to 5000mA @13:03:47.79 Powering core bus @13:03:50.14 SPR Module 1, FW Rev. B, FPGA Rev. C @13:03:50.58 Archive.configure :default @13:03:50.76 Air.configure :default @13:03:50.93 Lysis1.configure :default @13:03:51.15 Lysis2.configure :default @13:03:51.35 Plunge.configure :default @13:03:51.53 SamplePump.configure :default @13:03:51.70 Toroid.configure :default @13:03:51.87 Carousel.configure :default @13:03:52.02 Pressure.configure :default @13:03:52.18 ExtraPump.configure :default @13:03:52.30 PinPort.configure :default @13:03:52.44 All elf controllers running firmware version 4.84 @13:03:52.48 Gate.power :motor,:ON,limit to 5000mA @13:03:52.86 Archive.home.jog 0 @13:03:53.69 PinPort.home.jog 0 @13:03:53.78 Toroid.home.jog 0 @13:03:53.84 Plunge.home.jog 0 @13:03:53.89 Lysis1.home.jog 0 @13:03:54.00 Air.home.jog 0 @13:03:54.07 Lysis2.home.jog 0 @13:03:54.16 Carousel.home.jog 0 @13:03:55.74 Archive.in :homing @13:03:56.12 Archive.jog 1387 @13:03:56.22 Toroid.in :homing @13:03:56.28 Lysis2.in :homing @13:03:56.33 Air.in :homing @13:03:56.45 Plunge.in :homing @13:03:56.54 Lysis1.in :homing @13:03:56.65 PinPort.in :homing @13:03:57.11 Lysis2.jog 1394 @13:03:57.17 Toroid.jog 579 @13:03:57.25 Plunge.jog 1564 @13:03:57.29 Air.jog 1370 @13:03:57.37 PinPort.jog -107 @13:03:57.46 Lysis1.jog 1386 @13:03:58.01 Archive.setRawPosition! 50 @13:03:58.12 Archive.seek :home @13:03:58.28 PinPort.setRawPosition! 0 @13:03:58.38 PinPort.seek :home @13:03:58.55 Archive.in :default @13:03:58.58 PinPort.in :default @13:03:58.84 Lysis2.setRawPosition! 50 @13:03:58.98 Lysis2.seek :home @13:03:59.18 Plunge.setRawPosition! 50 @13:03:59.24 Lysis1.setRawPosition! 50 @13:03:59.41 Air.setRawPosition! 50 @13:03:59.60 Plunge.seek :home @13:03:59.76 Lysis1.seek :home @13:03:59.88 Air.seek :home @13:04:00.21 Toroid.setRawPosition! 0 @13:04:00.24 Plunge.in :default @13:04:00.33 Lysis2.in :default @13:04:00.57 Toroid.seek :home @13:04:00.82 Air.in :default @13:04:00.89 Lysis1.in :default @13:04:01.16 Toroid.in :default @13:04:01.44 Toroid.seek :clear @13:04:03.11 Carousel.in :default @13:04:03.31 Carousel.home.seek 22 @13:04:03.65 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:04:06.92 -> Power.sampler false @13:04:10.36 -> Power.sampler :ON @13:04:10.38 Gate.power :sampler,:ON true @13:04:17.16 -> #YES!! @13:04:28.03 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:04:28.07 Bypass.open @13:04:28.90 Intake.open @13:04:29.69 SamplePump.setPosition! 0ml @13:04:29.87 SamplePump.seek 30ml @13:05:06.79 Bypass.close @13:05:07.56 Exhaust.open @13:05:08.46 SamplePump.seek 55ml @13:05:39.30 delay 1 second @13:05:40.33 Intake.close @13:05:40.44 Exhaust.close @13:05:41.23 Sampling Loop filled with water @13:05:41.26 Refilling sample & bypass lines with fluid. @13:05:42.66 Toroid.seek :clear @13:05:42.81 Carousel.in :default @13:05:43.04 Carousel.home.seek 22 @13:05:43.36 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:05:47.08 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:05:47.10 Bypass.open @13:05:47.96 Intake.open @13:05:48.70 SamplePump.setPosition! 0ml @13:05:48.87 SamplePump.seek 30ml @13:06:25.76 Bypass.close @13:06:26.53 Exhaust.open @13:06:27.37 SamplePump.seek 55ml @13:06:58.20 delay 1 second @13:06:59.23 Intake.close @13:06:59.35 Exhaust.close @13:07:00.23 Sampling Loop filled with water @13:07:00.26 Refilling sample & bypass lines with fluid. @13:07:01.20 Toroid.seek :clear @13:07:01.36 Carousel.in :default @13:07:01.55 Carousel.home.seek 22 @13:07:01.89 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:07:04.53 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:07:04.54 Bypass.open @13:07:05.36 Intake.open @13:07:06.12 SamplePump.setPosition! 0ml @13:07:06.30 SamplePump.seek 30ml @13:07:43.19 Bypass.close @13:07:43.98 Exhaust.open @13:07:44.82 SamplePump.seek 55ml @13:08:15.64 delay 1 second @13:08:16.68 Intake.close @13:08:16.79 Exhaust.close @13:08:17.76 Sampling Loop filled with water 3 @13:08:39.23 -> BACsampler.prime 300,100 @13:08:39.39 Priming sample loop w/300ml, bypass w/100ml @13:08:39.41 SamplePump.setPosition! 0ml @13:08:39.55 Bypass.open @13:08:41.15 Intake.open @13:08:41.98 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:08:44.58 Vacuum tare is 56% of full scale @13:08:44.81 Pressure.in :default @13:08:44.93 Pressure.hold @13:08:45.82 SamplePump.in :prime @13:08:45.96 SamplePump.seek 100ml,5:16 @13:10:47.73 Pressure.stop @13:10:47.85 SamplePump.setPosition! 0ml @13:10:47.95 Exhaust.open @13:10:49.44 Bypass.close @13:10:51.12 Pressure.in :default @13:10:51.26 Pressure.hold @13:10:51.63 SamplePump.in :prime @13:10:51.77 SamplePump.seek 300ml,14:48 @13:11:44.56 -> loop{puts Sampler.psi} @13:11:45.11 -> exit @13:11:45.18 Session Ended @13:16:56.36 Pressure.stop BACsampler @13:17:00.80 UserAbort in nadia -- User Abort!! @13:18:38.23 -> start {fire :microSPR_phyto} # @13:18:39.29 Toroid.seek :clear @13:18:39.48 Carousel.in :default @13:18:39.71 Carousel.home.seek 22 @13:18:40.02 Selecting Cartridge 22 @13:18:40.39 Carousel.seek 22 @13:18:40.69 Lysis1.in :fast @13:18:40.80 Lysis1.seek :locked @13:18:43.48 Plunge.seek :locked @13:18:43.71 Air.in :fast @13:18:43.81 Air.seek :locked @13:18:45.43 Carousel.reconfigure :locked @13:18:45.64 Powering cartridge bus @13:18:48.31 Gate.setIR 1750,5000,0,6 @13:18:48.44 Gate.setIR 0,5000,0,6 @13:18:48.57 PuckHeater.configure :default #ambient 27.5C @13:18:48.68 Gate.power :heat,:ON,limit to 5000mA @13:18:51.39 Mounted lysis Cartridge board#Q05090 @13:18:51.46 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:18:51.86 Preparing to filter cartridge 22 @13:18:52.19 Bypass.open @13:18:52.95 Intake.close @13:18:54.42 Toroid.in :hold @13:18:54.53 Toroid.seek :crack @13:18:56.14 Toroid.seek :cartridge @13:18:57.31 Toroid.in :default @13:18:57.42 Toroid.seek :clear @13:18:59.50 Pressurizing cartridge to 26psi above 13.4psia @13:18:59.53 SamplePump.setPosition! 0ml @13:18:59.66 Intake.open @13:19:00.34 Bypass.close @13:19:01.12 Exhaust.close @13:19:02.03 Pressure.in :default @13:19:02.14 Pressure.hold @13:19:02.44 SamplePump.in :charge @13:19:02.56 SamplePump.seek 2.3ml,42 seconds @13:19:15.82 Pressure.stop @13:19:15.94 Intake.close @13:19:16.71 Pressurized cartridge with 0.790ml to 26.1psi @13:19:16.73 Isolated @33.8psia (20.4psi) @13:19:16.78 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @13:19:16.86 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:19:26.49 Cartridge pressure fell 19% to 27.4psia @13:19:26.69 Toroid.in :hold @13:19:26.80 Toroid.seek :bypass @13:19:28.50 Toroid.in :default @13:19:28.64 Toroid.seek :clear @13:19:30.43 Cartridge::Sampler::Leaky in FILTERING -- Retained only 27.4psia @13:19:30.45 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @13:19:33.30 Gate.power :sampler,:OFF @13:25:46.23 -> Power.sampler :ON @13:25:46.26 Gate.power :sampler,:ON true @13:27:08.30 -> require 'vacuumCart'; 3.times{refillSampleLines}; BACsampler.prime 300,100 @13:27:08.34 Refilling sample & bypass lines with fluid. @13:27:09.91 Toroid.seek :clear @13:27:10.03 Carousel.in :default @13:27:10.20 Carousel.home.seek 22 @13:27:10.69 Carousel.reconfigure :locked @13:27:10.97 Gate.setIR 1750,5000,0,6 @13:27:11.12 Gate.setIR 0,5000,0,6 @13:27:11.23 PuckHeater.configure :default #ambient 27.5C @13:27:11.34 Gate.power :heat,:ON,limit to 5000mA @13:27:14.03 Mounted lysis Cartridge board#Q05090 @13:27:14.10 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:27:14.18 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:27:16.95 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:27:16.97 Bypass.open @13:27:17.83 Intake.open @13:27:18.53 SamplePump.setPosition! 0ml @13:27:18.69 SamplePump.in :default @13:27:18.80 SamplePump.seek 30ml @13:27:55.67 Bypass.close @13:27:56.43 Exhaust.open @13:27:57.28 SamplePump.seek 55ml @13:28:13.91 -> Can Can@13:26:28, 27.8C, 43% humidity, 14.4psia, 14.845V, 0.575A, 11.205Ah, 8.54W @13:28:28.09 delay 1 second @13:28:29.11 Intake.close @13:28:29.22 Exhaust.close @13:28:30.08 Sampling Loop filled with water @13:28:30.10 Refilling sample & bypass lines with fluid. @13:28:30.98 Toroid.seek :clear @13:28:31.14 Carousel.in :default @13:28:31.31 Carousel.home.seek 22 @13:28:31.77 Carousel.reconfigure :locked @13:28:32.05 Gate.setIR 1750,5000,0,6 @13:28:32.19 Gate.setIR 0,5000,0,6 @13:28:32.29 PuckHeater.configure :default #ambient 27.5C @13:28:32.40 Gate.power :heat,:ON,limit to 5000mA @13:28:33.15 Mounted lysis Cartridge board#Q05090 @13:28:33.22 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:28:33.30 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:28:43.10 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:28:43.13 Bypass.open @13:28:43.93 Intake.open @13:28:44.63 SamplePump.setPosition! 0ml @13:28:44.79 SamplePump.seek 30ml @13:29:21.68 Bypass.close @13:29:22.45 Exhaust.open @13:29:23.29 SamplePump.seek 55ml @13:29:54.68 delay 1 second @13:29:55.72 Intake.close @13:29:55.80 Exhaust.close @13:29:56.60 Sampling Loop filled with water @13:29:56.63 Refilling sample & bypass lines with fluid. @13:29:57.54 Toroid.seek :clear @13:29:57.68 Carousel.in :default @13:29:57.85 Carousel.home.seek 22 @13:29:58.32 Carousel.reconfigure :locked @13:29:58.60 Gate.setIR 1750,5000,0,6 @13:29:58.73 Gate.setIR 0,5000,0,6 @13:29:58.83 PuckHeater.configure :default #ambient 27.5C @13:29:58.95 Gate.power :heat,:ON,limit to 5000mA @13:29:59.66 Mounted lysis Cartridge board#Q05090 @13:29:59.75 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:29:59.81 Waiting for errera to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @13:30:05.59 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @13:30:05.62 Bypass.open @13:30:06.46 Intake.open @13:30:07.14 SamplePump.setPosition! 0ml @13:30:07.31 SamplePump.seek 30ml @13:30:44.16 Bypass.close @13:30:44.97 Exhaust.open @13:30:45.82 SamplePump.seek 55ml @13:31:16.63 delay 1 second @13:31:17.67 Intake.close @13:31:17.77 Exhaust.close @13:31:18.59 Sampling Loop filled with water @13:31:18.73 Priming sample loop w/300ml, bypass w/100ml @13:31:18.75 SamplePump.setPosition! 0ml @13:31:18.84 Bypass.open @13:31:20.49 Intake.open @13:31:22.02 Pressure.hold @13:31:22.32 SamplePump.in :prime @13:31:22.44 SamplePump.seek 100ml,5:16 @13:31:30.71 -> Can Can@13:30:27, 27.9C, 42% humidity, 14.4psia, 14.809V, 1.029A, 11.262Ah, 15.24W @13:33:05.42 -> loop{puts Sampler.psi;Delay.sleep .5} @13:33:24.23 Pressure.stop @13:33:24.34 SamplePump.setPosition! 0ml @13:33:24.42 Exhaust.open @13:33:25.94 Bypass.close @13:33:27.55 Pressure.in :default @13:33:27.65 Pressure.hold @13:33:28.12 SamplePump.in :prime @13:33:28.24 SamplePump.seek 300ml,14:48 @13:39:31.82 Pressure.stop BACsampler @13:39:48.31 UserAbort in nadia -- User Abort!! @13:40:27.67 -> start {fire :microSPR_phyto} # @13:40:27.92 Selecting Cartridge 20 @13:40:28.19 Gate.power :heat,:OFF @13:40:28.22 Gate.power :cartridge,:OFF @13:40:28.38 Carousel.in :default @13:40:28.56 Carousel.home.seek 22 @13:40:29.09 Air.in :unlocking @13:40:29.30 Air.seek :clear @13:40:30.76 Lysis1.in :unlocking @13:40:30.97 Lysis1.seek :clear @13:40:31.05 Plunge.in :unlocking @13:40:31.25 Plunge.seek :clear @13:40:32.47 Carousel.seek 20 @13:40:33.28 Lysis1.in :fast @13:40:33.39 Lysis1.seek :locked @13:40:36.04 Plunge.in :default @13:40:36.17 Plunge.seek :locked @13:40:36.37 Air.in :fast @13:40:36.49 Air.seek :locked @13:40:38.10 Carousel.reconfigure :locked @13:40:38.33 Powering cartridge bus @13:40:41.18 Gate.setIR 1750,5000,0,6 @13:40:41.32 Gate.setIR 0,5000,0,6 @13:40:41.43 PuckHeater.configure :default #ambient 27.5C @13:40:41.54 Gate.power :heat,:ON,limit to 5000mA @13:40:42.25 Mounted lysis Cartridge board#Q05065 @13:40:42.33 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:40:42.71 Preparing to filter cartridge 20 @13:40:42.75 Cmd::Error in detached from errera#2 -- Cannot process while in READY state @13:40:42.87 Waiting to Resume from Checkpoint "Selecting Cartridge 22" @13:40:43.20 Bypass.open @13:40:43.99 Intake.close @13:40:44.82 Toroid.in :hold @13:40:44.93 Toroid.seek :crack @13:40:46.51 Toroid.seek :cartridge @13:40:47.67 Toroid.in :default @13:40:47.78 Toroid.seek :clear @13:40:49.86 Pressurizing cartridge to 26psi above 13.4psia @13:40:49.88 SamplePump.setPosition! 0ml @13:40:50.00 Intake.open @13:40:50.71 Bypass.close @13:40:51.46 Exhaust.close @13:40:52.34 Pressure.in :default @13:40:52.45 Pressure.hold @13:40:52.74 SamplePump.in :charge @13:40:52.86 SamplePump.seek 2.3ml,42 seconds @13:41:06.12 Pressure.stop @13:41:06.24 Intake.close @13:41:07.10 Pressurized cartridge with 0.789ml to 26.2psi @13:41:07.12 Isolated @33.4psia (19.9psi) @13:41:07.17 Verifying cartridge holds at least 0.65..0.85 of 33.4psia @13:41:07.26 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:41:11.94 Sampling 50.0ml @13:41:11.96 SamplePump.setPosition! 0ml @13:41:12.08 Exhaust.open @13:41:12.83 Intake.open @13:41:13.70 Pressure.in :default @13:41:13.81 Pressure.hold @13:41:14.64 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @13:41:14.88 SamplePump.in :sample @13:41:15.03 SamplePump.seek 50ml,4:41 @13:41:18.01 Cartridge 20 filtering 1/50ml at 0.51ml/s with 26.3psi vacuum @13:42:18.02 Cartridge 20 filtering 29/50ml at 0.40ml/s with 20.4psi vacuum @13:43:09.50 Pressure.stop @13:43:09.71 Intake.close @13:43:09.76 Exhaust.close @13:43:10.10 Toroid.in :hold @13:43:10.22 Toroid.seek :bypass @13:43:11.91 Toroid.in :default @13:43:12.03 Toroid.seek :clear @13:43:13.69 Sampled 50.0ml @13:43:15.45 SPR.command "read_sequences" @13:43:15.54 SPR.command "stats_on" @13:43:15.74 SPR.command "start 0 1" @13:43:16.43 SPR.start /var/log/esp/spr23aug1313h.spr.bz2 @13:43:16.87 Gate.power :sampler,:OFF @13:43:18.33 Handoff.select :waste @13:43:25.10 Syringe tare is 18% of full scale @13:43:25.17 PinPort.in :locking @13:43:25.28 PinPort.seek :locked @13:43:27.87 Archive.in :fast @13:43:27.93 Lysis2.in :fast @13:43:28.04 Lysis1.seek 1350uL @13:43:28.29 Archive.seek :full @13:43:28.42 Lysis2.seek 950uL @13:43:33.19 Plunge.seek :reagent @13:43:34.81 Evacuating puck with filter bubble point of 50psi @13:43:34.85 PinPort.seek :locked @13:43:35.07 Handoff.select :waste @13:43:41.69 Air syringe pumping up to 1 strokes to reach 8psi @13:43:41.70 Air stroke #1 of 1 @13:43:41.86 Air.in :pressurize @13:43:41.97 Air.seek :pressurized @13:43:47.77 0.8psi after pressurize @13:43:47.80 Air.in :compress @13:43:47.94 Air.seek :evac @13:43:51.10 1.1psi after compress @13:44:01.70 0.9psi after compress (stable) @13:44:01.72 Air.in :default @13:44:01.85 Air.hold @13:44:02.13 at 0.8psi Opened to waste @13:44:02.68 Handoff.select :blocked @13:44:03.01 Archive syringe pumping up to 8 strokes to reach 50psi @13:44:03.04 Archive stroke #1 of 8 @13:44:03.23 Archive.in :pressurize @13:44:03.38 Archive.seek :pressurized @13:44:15.93 5.3psi after pressurize @13:44:21.53 5.5psi after pressurize (stable) @13:44:21.57 Archive.in :compress @13:44:21.72 Archive.seek :empty @13:44:36.43 21.2psi after compress @13:44:42.44 Session Begun @13:44:48.16 -> slot 22 {22 => { Type: :microSPR_phyto, State: :leaky}} @13:44:50.56 -> slot 21 @13:44:50.60 25.9psi after compress (stable) {21 => { Type: :archiveHiBiomass_bac, State: :dry}} @13:44:50.75 Archive.in :gulp @13:44:50.96 Archive.seek :topVent @13:44:57.32 26.4psi after gulp @13:45:02.94 26.4psi after gulp (stable) @13:45:02.96 Archive stroke #2 of 8 @13:45:03.21 Archive.in :pressurize @13:45:03.35 Archive.seek :pressurized @13:45:15.90 26.2psi after pressurize @13:45:21.51 26.2psi after pressurize (stable) @13:45:21.54 Archive.in :compress @13:45:21.81 Archive.seek :empty @13:45:22.31 -> slot 20 {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug1313h.spr.bz2}} @13:45:36.51 40.4psi after compress @13:45:50.15 44.6psi after compress (stable) @13:45:50.21 Archive.in :gulp @13:45:50.38 Archive.seek :topVent @13:45:56.71 44.9psi after gulp @13:46:00.81 -> slots {[32,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :microSPR_phyto, State: :lysing, filtered: 50.0, spr: spr23aug1313h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1310h.spr.bz2}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @13:46:03.53 45.0psi after gulp (stable) @13:46:03.55 Archive stroke #3 of 8 @13:46:03.77 Archive.in :pressurize @13:46:03.91 Archive.seek :pressurized @13:46:16.47 44.8psi after pressurize @13:46:22.09 44.8psi after pressurize (stable) @13:46:22.16 Archive.in :compress @13:46:22.32 Archive.seek :empty @13:46:33.43 Reached 50.3 of 50.0psi after pumping 5111uL (2.95 strokes) @13:46:33.45 Archive.in :default @13:46:33.64 Archive.hold @13:46:33.82 Handoff.select :waste @13:46:45.12 delay 1:00 @13:47:45.15 Handoff.select :blocked @13:48:07.92 Handoff.select :waste @13:48:08.12 0.2psi at evacEnd @13:48:08.14 Handoff.select :blocked @13:48:08.42 Archive.in :fast @13:48:08.55 Archive.seek :topVent @13:48:22.03 Stable @0.4psi after Handoff blocked @13:48:22.06 Archive syringe pumping up to 8 strokes to reach 30psi @13:48:22.10 Archive stroke #1 of 8 @13:48:22.33 Archive.in :pressurize @13:48:22.51 Archive.seek :pressurized @13:48:35.11 1.1psi after pressurize @13:48:35.18 Archive.in :compress @13:48:35.32 Archive.seek :empty @13:48:50.01 14.8psi after compress @13:49:00.62 15.7psi after compress (stable) @13:49:00.69 Archive.in :gulp @13:49:00.83 Archive.seek :topVent @13:49:07.17 15.8psi after gulp @13:49:12.78 15.8psi after gulp (stable) @13:49:12.80 Archive stroke #2 of 8 @13:49:13.04 Archive.in :pressurize @13:49:13.17 Archive.seek :pressurized @13:49:26.42 15.7psi after pressurize @13:49:26.46 Archive.in :compress @13:49:26.63 Archive.seek :empty @13:49:41.35 29.4psi after compress @13:49:51.95 30.3psi after compress (stable) @13:49:52.02 Archive.in :gulp @13:49:52.16 Archive.seek :topVent @13:49:52.50 Reached 30.3 of 30.4psi after pumping 3460uL (2.00 strokes) @13:49:52.52 Archive.in :default @13:49:52.68 Archive.hold @13:49:52.84 Handoff.select :waste @13:50:02.27 delay 1:00 @13:51:02.30 Handoff.select :blocked @13:51:25.10 Handoff.select :waste @13:51:25.41 0.3psi at evacEnd @13:51:25.45 Air.seek :fullVent @13:51:26.97 Archive.in :fast @13:51:27.15 Archive.seek :topVent @13:51:34.31 Seawater evacuated, Pressure above/below filter released. @13:51:34.52 Lysis1.in :slow @13:51:34.66 Lysis1.seek 1050uL @13:52:04.74 delay 5 seconds @13:52:09.77 Handoff.select :blocked @13:52:10.05 Lysis1.seek 450uL @13:53:10.05 delay 3 seconds @13:53:13.16 Air.in mix with 1.40048 parts Lysis1 @13:53:14.05 Air.seek :evac @13:53:14.11 Lysis1.seek 300uL @13:53:29.35 Lysis1.seek :empty @13:53:59.53 Lysis buffer delivered @31.9psi @13:53:59.56 Polling Can every 2:00 @13:54:00.18 PuckHeater.configure :default #ambient 28.2C @13:54:00.30 PuckHeater.seek 79.5C @13:54:49.52 PuckHeater.hold 2:00,79.5C @13:56:49.35 Polling Can every 2:00 @13:56:49.93 PuckHeater.configure :default #ambient 28.2C @13:56:50.06 PuckHeater.hold 30 seconds,78.5C @13:57:20.22 Polling Can every 2:00 @13:57:20.78 PuckHeater.configure :default #ambient 28.2C @13:57:20.90 PuckHeater.hold 30 seconds,77.9C @13:57:37.97 -> can Can@13:57:38, 28.2C, 42% humidity, 14.4psia, 14.820V, 0.769A, 11.708Ah, 11.40W @13:57:51.09 Polling Can every 2:00 @13:57:51.64 PuckHeater.configure :default #ambient 28.2C @13:57:51.77 PuckHeater.hold 10:00,77.4C @13:58:13.10 -> can Can@13:58:13, 28.2C, 42% humidity, 14.4psia, 14.821V, 0.760A, 11.715Ah, 11.27W @13:58:16.07 -> Heat Puck Heater at 77.4C @14:07:50.10 Lysis complete. Syringe pressure @25.7psi @14:07:50.20 Archive syringe pumping up to 5 strokes to reach 40psi @14:07:50.23 Archive stroke #1 of 5 @14:07:50.41 Archive.in :pressurize @14:07:50.57 Archive.seek :pressurized @14:08:03.13 24.4psi after pressurize @14:08:08.73 24.2psi after pressurize (stable) @14:08:08.81 Archive.in :compress @14:08:08.93 Archive.seek :empty @14:08:14.35 Reached 40.8 of 40.0psi after pumping 1528uL (0.88 strokes) @14:08:14.37 Archive.in :default @14:08:14.53 Archive.hold @14:08:14.67 delay 2 seconds @14:08:16.70 Archive.in :fast @14:08:16.84 Archive.seek :full @14:08:23.37 Lysis1.seek 138uL @14:08:37.30 delay 1 second @14:08:38.35 Archive.seek :full @14:08:38.42 Lysis1.seek :empty @14:08:52.45 Handoff.select :blocked @14:08:52.64 Initial air pressure @55.0psi @14:08:52.77 Lysis1.in :extract @14:08:53.02 Lysis1.seek 600uL,nil @14:08:55.64 Archive syringe pumping up to 6 strokes to reach 25psi @14:08:55.66 Archive stroke #1 of 6 @14:08:55.91 Archive.in :pressurize @14:08:56.07 Archive.seek :pressurized @14:09:08.65 13.1psi after pressurize @14:09:20.21 Archive.in :default @14:09:20.30 Archive.hold @14:09:20.61 Initial 600uL lysate @15.1psi @14:09:20.68 Handoff.select :blocked @14:09:20.84 Initial air pressure @15.7psi @14:09:20.99 Lysis1.seek 825uL,nil @14:09:22.50 Archive syringe pumping up to 6 strokes to reach 17psi @14:09:22.52 Archive stroke #1 of 6 @14:09:22.73 Archive.in :compress @14:09:22.90 Archive.seek :empty @14:09:32.07 Archive.in :default @14:09:32.15 Archive.hold @14:09:32.66 Recovered 825uL lysate @15.7psi @14:09:32.75 Air.in :halfSpeed @14:09:32.89 Air.seek :fullVent @14:09:35.17 Puck Top Vented, bottom @12.4psi @14:09:35.19 Handoff.select :waste @14:09:35.42 delay 5 seconds @14:09:40.51 Handoff open to waste, bottom @0.5psi @14:09:40.57 Air.in :default @14:09:40.70 Air.seek :evac @14:09:42.19 Lysis2.in :DAmix @14:09:42.40 Lysis1.in :DAmix @14:09:43.17 Lysis2.seek 700uL @14:09:43.23 Lysis1.seek 575uL @14:10:18.42 delay 5 seconds @14:10:23.44 Handoff.select SPR @14:10:23.65 SPR.command "start 2 14" @14:10:24.21 SPR stabilized @14:10:24.45 delay 10 seconds @14:10:30.88 -> exit @14:10:30.91 Session Ended @14:10:34.48 SPR.command "start 2 0" @14:10:37.58 Waiting up to 11:00 for SPR to enter state 1 @14:12:35.24 SPR.command "hold_on" @14:12:37.16 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:12:37.97 Lysis2.seek 400uL @14:12:38.02 Lysis1.seek 275uL @14:13:20.10 delay 15 seconds @14:13:35.12 SPR.command "hold_off" @14:13:38.75 Waiting up to 38:00 for SPR to enter state 7 @14:27:58.42 SPRsummary:-10.1RIU,2.72RIU,88.1RIU,118RIU,high,124ng/L,216ng/L after 2833s @14:31:59.11 Session Ended @14:39:28.85 SPR analysis completed @14:39:35.95 Ended log spr23aug1313h.spr.bz2 @14:39:35.97 SPR.command "stats_off" @14:39:37.08 Handoff.select :close @14:39:37.26 Gate.power :heat,:OFF @14:39:37.28 Gate.power :cartridge,:OFF @14:39:37.41 PinPort.in :unlocking @14:39:37.50 PinPort.seek :clear @14:39:39.46 Carousel.in :default @14:39:39.62 Carousel.home.seek 20 @14:39:39.99 Plunge.seek :locked @14:39:40.91 Archive.in :unlocking @14:39:41.12 Archive.seek :clear @14:39:41.26 Air.in :unlocking @14:39:41.44 Air.seek :clear @14:39:41.51 Lysis1.in :unlocking @14:39:41.74 Lysis1.seek :clear @14:39:41.80 Lysis2.in :unlocking @14:39:41.96 Lysis2.seek :clear @14:39:45.88 Gate.power :motor,:OFF @14:39:45.89 Gate.power :core,:OFF @14:39:45.94 Gate.power :analytic1,:OFF @14:39:45.98 Gate.power :analytic2,:OFF @14:39:46.01 Gate.power :main,:OFF @14:39:46.03 Safely stopped and ready to power off @14:39:58.20 -> ESP.ready!; SPR.start; delay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.command “start 2 14"; SPR.stop; delay 10; Cmd.stop @14:39:58.24 SyntaxError in errera -- compile error errera:26: Invalid char `\342' in expression errera:26: Invalid char `\200' in expression errera:26: Invalid char `\234' in expression errera:26: syntax error, unexpected tINTEGER, expecting $end ...ay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.co... ^ @14:41:51.64 -> ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @14:41:51.71 Gate.power :sampler,:OFF,limit to 3500mA @14:41:51.74 Gate.power :main,:ON,limit to 5000mA @14:41:51.77 Gate.power :analytic1,:ON,limit to 5000mA @14:41:51.80 Gate.power :analytic2,:ON,limit to 5000mA @14:41:52.57 Powering core bus @14:41:54.25 SPR Module 1, FW Rev. B, FPGA Rev. C @14:41:55.34 Archive.configure :default @14:41:55.46 Air.configure :default @14:41:55.55 Lysis1.configure :default @14:41:55.64 Lysis2.configure :default @14:41:55.76 Plunge.configure :default @14:41:55.88 SamplePump.configure :default @14:41:56.01 Toroid.configure :default @14:41:56.12 Carousel.configure :default @14:41:56.24 Pressure.configure :default @14:41:56.36 ExtraPump.configure :default @14:41:56.45 PinPort.configure :default @14:41:56.55 All elf controllers running firmware version 4.84 @14:41:56.58 Gate.power :motor,:ON,limit to 5000mA @14:41:56.79 Archive.home.jog 0 @14:41:57.68 PinPort.home.jog 0 @14:41:57.73 Toroid.home.jog 0 @14:41:57.80 Plunge.home.jog 0 @14:41:57.84 Lysis1.home.jog 0 @14:41:57.91 Lysis2.home.jog 0 @14:41:57.93 Air.home.jog 0 @14:41:57.98 Carousel.home.jog 0 @14:41:58.77 Archive.in :homing @14:41:59.12 Archive.jog 1387 @14:41:59.40 PinPort.in :homing @14:41:59.43 Toroid.in :homing @14:41:59.48 Plunge.in :homing @14:41:59.53 Lysis2.in :homing @14:41:59.58 Air.in :homing @14:41:59.66 Lysis1.in :homing @14:41:59.94 PinPort.jog -107 @14:42:00.03 Plunge.jog 1577 @14:42:00.10 Toroid.jog 589 @14:42:00.15 Lysis2.jog 1394 @14:42:00.20 Air.jog 1365 @14:42:00.25 Lysis1.jog 1388 @14:42:00.73 PinPort.setRawPosition! 0 @14:42:00.75 Archive.setRawPosition! 50 @14:42:00.88 PinPort.seek :home @14:42:00.93 Archive.seek :home @14:42:01.17 PinPort.in :default @14:42:01.36 Archive.in :default @14:42:01.64 Lysis2.setRawPosition! 50 @14:42:01.68 Air.setRawPosition! 50 @14:42:01.79 Lysis2.seek :home @14:42:01.90 Air.seek :home @14:42:02.07 Plunge.setRawPosition! 50 @14:42:02.09 Lysis1.setRawPosition! 50 @14:42:02.22 Plunge.seek :home @14:42:02.29 Lysis1.seek :home @14:42:02.53 Toroid.setRawPosition! 0 @14:42:02.56 Lysis2.in :default @14:42:02.60 Air.in :default @14:42:03.41 Toroid.seek :home @14:42:03.59 Toroid.in :default @14:42:03.63 Plunge.in :default @14:42:03.67 Lysis1.in :default @14:42:04.16 Toroid.seek :clear @14:42:05.81 Carousel.in :default @14:42:05.97 Carousel.home.seek 20 @14:42:07.43 SPR.command "read_sequences" @14:42:07.50 SPR.command "stats_on" @14:42:08.32 delay 2 seconds @14:42:09.12 SPR.start /var/log/esp/spr23aug1314h.spr.bz2 @14:42:10.34 SPR.command "start 3 0" @14:42:11.16 delay 15:00 @14:57:11.18 SPR.command "start 2 14" @14:57:18.10 Ended log spr23aug1314h.spr.bz2 @14:57:18.12 SPR.command "stats_off" @14:57:18.23 delay 10 seconds @14:57:28.42 Carousel.in :default @14:57:28.58 Carousel.home.seek 20 @14:57:28.95 Lysis1.in :fast @14:57:29.08 Lysis1.seek :locked @14:57:31.62 Plunge.seek :locked @14:57:32.99 Lysis1.in :unlocking @14:57:33.78 Lysis1.seek :clear @14:57:35.32 Gate.power :motor,:OFF @14:57:35.36 Gate.power :core,:OFF @14:57:35.38 Gate.power :analytic1,:OFF @14:57:35.42 Gate.power :analytic2,:OFF @14:57:35.45 Gate.power :main,:OFF @14:57:35.49 Safely stopped and ready to power off :STOPPED @15:01:24.91 -> Cartridge.release 32 1 @15:01:28.26 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1313h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1310h.spr.bz2}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @15:02:24.43 -> Cartridge.release 32 0 @15:02:29.22 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1313h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1310h.spr.bz2}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @15:03:41.62 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1313h.spr.bz2}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1310h.spr.bz2}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @15:05:35.19 -> start {fire :archiveHiBiomass_bac, :microSPR_phyto} # @15:05:35.49 Gate.power :sampler,:OFF,limit to 3500mA @15:05:35.52 Gate.power :main,:ON,limit to 5000mA @15:05:35.56 Gate.power :analytic1,:ON,limit to 5000mA @15:05:35.60 Gate.power :analytic2,:ON,limit to 5000mA @15:05:35.70 Powering core bus @15:05:38.05 SPR Module 1, FW Rev. B, FPGA Rev. C @15:05:38.48 Archive.configure :default @15:05:38.60 Air.configure :default @15:05:38.70 Lysis1.configure :default @15:05:38.79 Lysis2.configure :default @15:05:38.91 Plunge.configure :default @15:05:39.06 SamplePump.configure :default @15:05:39.19 Toroid.configure :default @15:05:39.31 Carousel.configure :default @15:05:39.43 Pressure.configure :default @15:05:39.56 ExtraPump.configure :default @15:05:39.66 PinPort.configure :default @15:05:39.75 All elf controllers running firmware version 4.84 @15:05:39.78 Gate.power :motor,:ON,limit to 5000mA @15:05:40.31 Archive.home.jog 0 @15:05:40.48 Air.home.jog 0 @15:05:40.60 Lysis1.home.jog 0 @15:05:40.71 Lysis2.home.jog 0 @15:05:40.93 Plunge.home.jog 0 @15:05:40.98 Toroid.home.jog 0 @15:05:41.70 PinPort.home.jog 0 @15:05:41.88 Carousel.home.jog 0 @15:05:42.45 Toroid.in :homing @15:05:42.52 Lysis2.in :homing @15:05:42.60 Air.in :homing @15:05:42.66 Plunge.in :homing @15:05:42.71 Archive.in :homing @15:05:42.76 Lysis1.in :homing @15:05:43.12 Toroid.jog 584 @15:05:43.19 Lysis2.jog 1394 @15:05:43.27 Air.jog 1365 @15:05:43.32 Plunge.jog 1574 @15:05:43.41 Lysis1.jog 1388 @15:05:43.48 Archive.jog 1386 @15:05:44.50 PinPort.in :homing @15:05:44.59 PinPort.jog -107 @15:05:44.74 Lysis2.setRawPosition! 50 @15:05:44.84 Lysis2.seek :home @15:05:44.97 Air.setRawPosition! 50 @15:05:45.01 PinPort.setRawPosition! 0 @15:05:45.19 PinPort.seek :home @15:05:45.22 Air.seek :home @15:05:45.55 PinPort.in :default @15:05:45.59 Toroid.setRawPosition! 0 @15:05:45.65 Lysis1.setRawPosition! 50 @15:05:45.69 Archive.setRawPosition! 50 @15:05:45.73 Lysis2.in :default @15:05:46.00 Toroid.seek :home @15:05:46.04 Lysis1.seek :home @15:05:46.12 Archive.seek :home @15:05:46.42 Plunge.setRawPosition! 50 @15:05:46.45 Air.in :default @15:05:46.50 Toroid.in :default @15:05:46.68 Plunge.seek :home @15:05:46.89 Archive.in :default @15:05:46.92 Lysis1.in :default @15:05:47.15 Plunge.in :default @15:05:47.53 Toroid.seek :clear @15:05:49.18 Carousel.in :default @15:05:49.34 Carousel.home.seek 20 @15:05:49.65 Selecting Cartridge 21 @15:05:49.99 Carousel.seek 21 @15:05:50.58 Lysis1.in :fast @15:05:50.67 Lysis1.seek :locked @15:05:53.33 Plunge.seek :locked @15:05:53.53 Air.in :fast @15:05:53.62 Air.seek :locked @15:05:55.22 Carousel.reconfigure :locked @15:05:55.42 Powering cartridge bus @15:05:58.09 Gate.setIR 1750,5000,0,6 @15:05:58.22 Gate.setIR 0,5000,0,6 @15:05:58.32 Gate.power :cartridge,:OFF @15:05:58.89 Mounted archive Cartridge @15:05:59.30 Preparing to filter cartridge 21 @15:05:59.57 Gate.power :sampler,:ON @15:05:59.69 Priming sample loop w/150ml, bypass w/50ml @15:05:59.72 SamplePump.setPosition! 0ml @15:05:59.80 Bypass.open @15:06:01.31 Intake.open @15:06:02.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:06:05.31 Vacuum tare is 56% of full scale @15:06:05.50 Pressure.in :default @15:06:05.59 Pressure.hold @15:06:05.86 Waiting for external sampler to finish priming... @15:06:05.88 delayUntil "15:09:29.59" @15:09:29.64 SamplePump.in :prime @15:09:29.75 SamplePump.seek 50ml,2:53 @15:10:30.77 Pressure.stop @15:10:30.85 SamplePump.setPosition! 0ml @15:10:30.93 Exhaust.open @15:10:32.44 Bypass.close @15:10:34.04 Pressure.in :default @15:10:34.14 Pressure.hold @15:10:34.48 SamplePump.in :prime @15:10:34.59 SamplePump.seek 150ml,7:39 @15:13:36.86 Pressure.stop @15:13:36.97 Bypass.open @15:13:37.77 Intake.close @15:13:38.60 Toroid.in :hold @15:13:38.70 Toroid.seek :crack @15:13:40.27 Toroid.seek :cartridge @15:13:41.42 Toroid.in :default @15:13:41.51 Toroid.seek :clear @15:13:43.56 Pressurizing cartridge to 26psi above 13.6psia @15:13:43.58 SamplePump.setPosition! 0ml @15:13:43.67 Intake.open @15:13:44.34 Bypass.close @15:13:45.07 Exhaust.close @15:13:45.93 Pressure.in :default @15:13:46.02 Pressure.hold @15:13:46.29 SamplePump.in :charge @15:13:46.38 SamplePump.seek 2.3ml,42 seconds @15:13:55.39 Pressure.stop @15:13:55.48 Intake.close @15:13:56.27 Pressurized cartridge with 0.360ml to 26.7psi @15:13:56.29 Isolated @33.8psia (20.2psi) @15:13:56.87 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @15:13:56.98 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:14:01.64 Cartridge pressure fell 1% to 33.6psia @15:14:01.77 Sampling 1000.0ml @15:14:01.79 SamplePump.setPosition! 0ml @15:14:01.88 Exhaust.open @15:14:02.62 Intake.open @15:14:03.53 Pressure.in :default @15:14:03.62 Pressure.hold @15:14:03.79 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @15:14:04.02 SamplePump.in :sample @15:14:04.13 SamplePump.seek 1000ml,1:23:51 @15:14:07.17 Cartridge 21 filtering 1/1000ml at 0.53ml/s with 24.1psi vacuum @15:15:07.17 Cartridge 21 filtering 31/1000ml at 0.44ml/s with 28.8psi vacuum @15:16:07.18 Cartridge 21 filtering 60/1000ml at 0.50ml/s with 24.7psi vacuum @15:17:07.18 Cartridge 21 filtering 87/1000ml at 0.46ml/s with 25.0psi vacuum @15:18:07.18 Cartridge 21 filtering 112/1000ml at 0.46ml/s with 27.3psi vacuum @15:19:07.19 Cartridge 21 filtering 136/1000ml at 0.41ml/s with 24.5psi vacuum @15:20:07.18 Cartridge 21 filtering 160/1000ml at 0.39ml/s with 16.4psi vacuum @15:21:07.18 Cartridge 21 filtering 182/1000ml at 0.31ml/s with 27.9psi vacuum @15:22:07.17 Cartridge 21 filtering 203/1000ml at 0.37ml/s with 27.4psi vacuum @15:23:07.18 Cartridge 21 filtering 223/1000ml at 0.33ml/s with 15.3psi vacuum @15:24:07.18 Cartridge 21 filtering 242/1000ml at 0.28ml/s with 27.3psi vacuum @15:25:07.18 Cartridge 21 filtering 260/1000ml at 0.25ml/s with 25.9psi vacuum @15:26:07.18 Cartridge 21 filtering 277/1000ml at 0.25ml/s with 26.6psi vacuum @15:27:07.17 Cartridge 21 filtering 293/1000ml at 0.22ml/s with 24.4psi vacuum @15:28:07.18 Cartridge 21 filtering 308/1000ml at 0.20ml/s with 24.7psi vacuum @15:29:07.17 Cartridge 21 filtering 323/1000ml at 0.20ml/s with 25.6psi vacuum @15:30:07.17 Cartridge 21 filtering 336/1000ml at 0.22ml/s with 15.3psi vacuum @15:30:19.97 Flow averaged 0.35ml/s over 16:16 @15:30:20.04 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @15:30:20.64 Relieved excess vacuum in .7 seconds @15:30:21.35 SamplePump.seek 1000ml,55:35 @15:31:07.17 Cartridge 21 filtering 350/1000ml at 0.27ml/s with 28.3psi vacuum @15:31:21.83 Flow averaged 0.23ml/s over 1:02 @15:31:21.90 Waiting up to 13 seconds for vacuum to reduce from 28.0 to 18.0psi @15:31:22.51 Relieved excess vacuum in .7 seconds @15:31:22.55 SamplePump.seek 1000ml,54:26 @15:31:38.11 Flow averaged 0.22ml/s over 16 seconds @15:31:38.19 Waiting up to 12 seconds for vacuum to reduce from 27.4 to 18.0psi @15:31:38.80 Relieved excess vacuum in .7 seconds @15:31:38.86 SamplePump.seek 1000ml,54:09 @15:31:50.29 Flow averaged 0.22ml/s over 12 seconds @15:31:50.36 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @15:31:50.97 Relieved excess vacuum in .7 seconds @15:31:51.06 SamplePump.seek 1000ml,53:56 @15:32:02.47 Flow averaged 0.22ml/s over 12 seconds @15:32:02.55 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @15:32:03.17 Relieved excess vacuum in .7 seconds @15:32:03.21 SamplePump.seek 1000ml,53:44 @15:32:07.17 Cartridge 21 filtering 362/1000ml at 0.20ml/s with 30.0psi vacuum @15:32:13.27 Flow averaged 0.21ml/s over 11 seconds @15:32:13.34 Waiting up to 17 seconds for vacuum to reduce from 29.5 to 18.0psi @15:32:13.94 Relieved excess vacuum in .7 seconds @15:32:13.99 SamplePump.seek 1000ml,53:33 @15:32:22.70 Flow estimate reduced to 0.17ml/s @15:32:22.73 Intake.close @15:32:22.85 Exhaust.close @15:32:23.15 Toroid.in :hold @15:32:23.26 Toroid.seek :bypass @15:32:24.95 Toroid.in :default @15:32:25.10 Toroid.seek :clear @15:32:26.75 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @15:32:26.77 Pressure.stop @15:32:27.11 Sampled 365.4ml @15:32:27.80 Plunge.seek :reagent @15:32:28.64 Sample loop closed. Ready to Deliver Reagents @15:32:28.66 Archive.seek :sealed @15:32:30.27 Gate.power :sampler,:OFF @15:32:32.64 Archive.seek :evac @15:32:46.70 delay 1:00 @15:33:47.24 Archive.seek :clear @15:34:04.85 RNAlater soak for 10:00 @15:35:35.15 -> exit @15:35:35.17 Session Ended @15:39:48.08 Session Begun @15:44:04.88 Archive.seek :tVent @15:44:07.36 delay 1 second @15:44:08.39 Archive.seek :evac @15:44:23.93 delay 10 seconds @15:44:33.97 Archive.seek :tVent @15:44:49.51 delay 1 second @15:44:50.54 Archive.seek :evac @15:45:06.08 delay 10 seconds @15:45:16.11 Sample preserved and evacuated @15:45:16.27 Selecting Cartridge 32 @15:45:16.55 Carousel.in :default @15:45:16.71 Carousel.home.seek 21 @15:45:17.13 Archive.in :unlocking @15:45:17.38 Archive.seek :clear @15:45:17.43 Air.in :unlocking @15:45:17.64 Air.seek :clear @15:45:22.03 Lysis1.in :unlocking @15:45:22.22 Lysis1.seek :clear @15:45:22.33 Plunge.in :unlocking @15:45:22.43 Plunge.seek :clear @15:45:23.88 Carousel.seek 32 @15:45:26.48 Lysis1.in :fast @15:45:26.57 Lysis1.seek :locked @15:45:29.27 Plunge.in :default @15:45:29.43 Plunge.seek :locked @15:45:29.82 Air.in :fast @15:45:29.98 Air.seek :locked @15:45:31.64 Carousel.reconfigure :locked @15:45:31.85 Powering cartridge bus @15:45:34.52 Gate.setIR 1750,5000,0,6 @15:45:34.65 Gate.setIR 0,5000,0,6 @15:45:34.74 PuckHeater.configure :default #ambient 28.2C @15:45:34.84 Gate.power :heat,:ON,limit to 5000mA @15:45:35.54 Mounted lysis Cartridge board#Q05052 @15:45:35.60 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:45:35.98 Preparing to filter cartridge 32 @15:45:36.28 Gate.power :sampler,:ON @15:45:36.92 Priming sample loop w/150ml, bypass w/50ml @15:45:36.94 SamplePump.setPosition! 0ml @15:45:37.07 Bypass.open @15:45:38.60 Intake.open @15:45:39.44 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:45:42.04 Vacuum tare is 56% of full scale @15:45:42.23 Pressure.in :default @15:45:42.32 Pressure.hold @15:45:42.57 Waiting for external sampler to finish priming... @15:45:42.59 delayUntil "15:49:06.30" @15:49:06.35 SamplePump.in :prime @15:49:06.46 SamplePump.seek 50ml,2:53 @15:50:07.50 Pressure.stop @15:50:07.59 SamplePump.setPosition! 0ml @15:50:07.68 Exhaust.open @15:50:09.13 Bypass.close @15:50:10.74 Pressure.in :default @15:50:10.84 Pressure.hold @15:50:11.20 SamplePump.in :prime @15:50:11.30 SamplePump.seek 150ml,7:39 @15:53:13.67 Pressure.stop @15:53:13.77 Bypass.open @15:53:14.53 Intake.close @15:53:15.38 Toroid.in :hold @15:53:15.47 Toroid.seek :crack @15:53:17.06 Toroid.seek :cartridge @15:53:18.21 Toroid.in :default @15:53:18.30 Toroid.seek :clear @15:53:20.36 Pressurizing cartridge to 26psi above 13.5psia @15:53:20.38 SamplePump.setPosition! 0ml @15:53:20.47 Intake.open @15:53:21.15 Bypass.close @15:53:21.90 Exhaust.close @15:53:22.77 Pressure.in :default @15:53:22.86 Pressure.hold @15:53:23.17 SamplePump.in :charge @15:53:23.28 SamplePump.seek 2.3ml,42 seconds @15:53:37.20 Pressure.stop @15:53:37.30 Intake.close @15:53:38.04 Pressurized cartridge with 0.860ml to 26.1psi @15:53:38.05 Isolated @33.8psia (20.2psi) @15:53:38.08 Verifying cartridge holds at least 0.65..0.85 of 33.8psia @15:53:38.15 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:53:42.86 Sampling 50.0ml @15:53:42.88 SamplePump.setPosition! 0ml @15:53:42.96 Exhaust.open @15:53:43.75 Intake.open @15:53:44.58 Pressure.in :default @15:53:44.68 Pressure.hold @15:53:44.86 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @15:53:45.11 SamplePump.in :sample @15:53:45.23 SamplePump.seek 50ml,4:41 @15:53:48.24 Cartridge 32 filtering 1/50ml at 0.56ml/s with 27.5psi vacuum @15:54:48.25 Cartridge 32 filtering 33/50ml at 0.46ml/s with 20.1psi vacuum @15:55:22.99 Pressure.stop @15:55:23.22 Intake.close @15:55:23.31 Exhaust.close @15:55:23.58 Toroid.in :hold @15:55:23.68 Toroid.seek :bypass @15:55:25.34 Toroid.in :default @15:55:25.44 Toroid.seek :clear @15:55:27.09 Sampled 50.0ml @15:55:28.80 SPR.command "read_sequences" @15:55:29.58 SPR.command "stats_on" @15:55:29.75 SPR.command "start 0 1" @15:55:30.24 Gate.power :sampler,:OFF @15:55:30.46 SPR.start /var/log/esp/spr23aug1315h.spr.bz2 @15:55:32.43 Handoff.select :waste @15:55:39.18 Syringe tare is 19% of full scale @15:55:39.22 PinPort.in :locking @15:55:39.32 PinPort.seek :locked @15:55:41.89 Archive.in :fast @15:55:41.94 Lysis2.in :fast @15:55:42.06 Lysis1.seek 1350uL @15:55:42.19 Archive.seek :full @15:55:42.30 Lysis2.seek 950uL @15:55:47.13 Plunge.seek :reagent @15:55:48.31 Evacuating puck with filter bubble point of 50psi @15:55:48.34 PinPort.seek :locked @15:55:48.47 Handoff.select :waste @15:55:55.12 Air syringe pumping up to 1 strokes to reach 8psi @15:55:55.13 Air stroke #1 of 1 @15:55:55.28 Air.in :pressurize @15:55:55.37 Air.seek :pressurized @15:56:01.15 1.0psi after pressurize @15:56:01.18 Air.in :compress @15:56:01.36 Air.seek :evac @15:56:04.41 1.0psi after compress @15:56:15.03 0.7psi after compress (stable) @15:56:15.06 Air.in :default @15:56:15.21 Air.hold @15:56:15.58 at 0.8psi Opened to waste @15:56:15.60 Handoff.select :blocked @15:56:15.78 Archive syringe pumping up to 8 strokes to reach 50psi @15:56:15.80 Archive stroke #1 of 8 @15:56:15.93 Archive.in :pressurize @15:56:16.02 Archive.seek :pressurized @15:56:28.56 3.2psi after pressurize @15:56:34.16 3.2psi after pressurize (stable) @15:56:34.19 Archive.in :compress @15:56:34.30 Archive.seek :empty @15:56:48.95 17.1psi after compress @15:56:59.56 18.3psi after compress (stable) @15:56:59.59 Archive.in :gulp @15:56:59.70 Archive.seek :topVent @15:57:05.99 18.4psi after gulp @15:57:11.59 18.4psi after gulp (stable) @15:57:11.60 Archive stroke #2 of 8 @15:57:11.75 Archive.in :pressurize @15:57:11.84 Archive.seek :pressurized @15:57:24.37 18.3psi after pressurize @15:57:29.97 18.4psi after pressurize (stable) @15:57:30.00 Archive.in :compress @15:57:30.12 Archive.seek :empty @15:57:44.80 32.2psi after compress @15:57:55.40 33.3psi after compress (stable) @15:57:55.43 Archive.in :gulp @15:57:55.54 Archive.seek :topVent @15:58:01.82 33.4psi after gulp @15:58:07.43 33.4psi after gulp (stable) @15:58:07.45 Archive stroke #3 of 8 @15:58:08.11 Archive.in :pressurize @15:58:08.23 Archive.seek :pressurized @15:58:20.76 33.2psi after pressurize @15:58:26.36 33.1psi after pressurize (stable) @15:58:26.39 Archive.in :compress @15:58:26.50 Archive.seek :empty @15:58:41.19 45.9psi after compress @15:58:51.79 47.0psi after compress (stable) @15:58:51.82 Archive.in :gulp @15:58:51.93 Archive.seek :topVent @15:58:58.78 47.4psi after gulp @15:59:04.38 47.4psi after gulp (stable) @15:59:04.39 Archive stroke #4 of 8 @15:59:04.54 Archive.in :pressurize @15:59:04.63 Archive.seek :pressurized @15:59:17.20 47.1psi after pressurize @15:59:22.85 47.0psi after pressurize (stable) @15:59:22.88 Archive.in :compress @15:59:23.01 Archive.seek :empty @15:59:31.17 Reached 50.3 of 50.0psi after pumping 6778uL (3.92 strokes) @15:59:31.19 Archive.in :default @15:59:31.35 Archive.hold @15:59:31.46 Handoff.select :waste @15:59:45.22 delay 1:00 @16:00:45.24 Handoff.select :blocked @16:01:08.00 Handoff.select :waste @16:01:08.16 0.4psi at evacEnd @16:01:08.18 Handoff.select :blocked @16:01:08.36 Archive.in :fast @16:01:08.45 Archive.seek :topVent @16:01:09.71 -> exit @16:01:09.74 Session Ended @16:01:21.67 Stable @0.5psi after Handoff blocked @16:01:21.70 Archive syringe pumping up to 8 strokes to reach 31psi @16:01:21.71 Archive stroke #1 of 8 @16:01:21.80 Archive.in :pressurize @16:01:21.86 Archive.seek :pressurized @16:01:34.39 1.3psi after pressurize @16:01:34.41 Archive.in :compress @16:01:34.47 Archive.seek :empty @16:01:49.12 13.4psi after compress @16:01:59.73 14.3psi after compress (stable) @16:01:59.78 Archive.in :gulp @16:01:59.87 Archive.seek :topVent @16:02:06.14 14.4psi after gulp @16:02:11.74 14.3psi after gulp (stable) @16:02:11.75 Archive stroke #2 of 8 @16:02:11.84 Archive.in :pressurize @16:02:11.91 Archive.seek :pressurized @16:02:24.47 14.3psi after pressurize @16:02:24.49 Archive.in :compress @16:02:24.56 Archive.seek :empty @16:02:39.18 27.1psi after compress @16:02:49.78 27.8psi after compress (stable) @16:02:49.81 Archive.in :gulp @16:02:49.88 Archive.seek :topVent @16:02:56.18 27.9psi after gulp @16:03:01.78 27.9psi after gulp (stable) @16:03:01.79 Archive stroke #3 of 8 @16:03:01.88 Archive.in :pressurize @16:03:01.95 Archive.seek :pressurized @16:03:14.45 27.6psi after pressurize @16:03:14.51 Archive.in :compress @16:03:14.59 Archive.seek :empty @16:03:19.67 Reached 30.7 of 30.5psi after pumping 4983uL (2.88 strokes) @16:03:19.69 Archive.in :default @16:03:19.75 Archive.hold @16:03:19.83 Handoff.select :waste @16:03:34.54 delay 1:00 @16:04:34.56 Handoff.select :blocked @16:04:57.27 Handoff.select :waste @16:04:57.39 0.5psi at evacEnd @16:04:57.41 Air.seek :fullVent @16:04:58.83 Archive.in :fast @16:04:58.90 Archive.seek :topVent @16:05:05.27 Seawater evacuated, Pressure above/below filter released. @16:05:05.37 Lysis1.in :slow @16:05:05.44 Lysis1.seek 1050uL @16:05:35.44 delay 5 seconds @16:05:40.47 Handoff.select :blocked @16:05:40.68 Lysis1.seek 450uL @16:06:40.56 delay 3 seconds @16:06:43.66 Air.in mix with 1.40048 parts Lysis1 @16:06:44.33 Air.seek :evac @16:06:44.42 Lysis1.seek 300uL @16:06:59.54 Lysis1.seek :empty @16:07:29.65 Lysis buffer delivered @10.7psi @16:07:29.67 Polling Can every 2:00 @16:07:30.21 PuckHeater.configure :default #ambient 27.6C @16:07:30.27 PuckHeater.seek 79.5C @16:08:19.15 PuckHeater.hold 2:00,79.5C @16:10:18.98 Polling Can every 2:00 @16:10:19.55 PuckHeater.configure :default #ambient 27.7C @16:10:19.61 PuckHeater.hold 30 seconds,78.5C @16:10:49.76 Polling Can every 2:00 @16:10:50.31 PuckHeater.configure :default #ambient 27.6C @16:10:50.38 PuckHeater.hold 30 seconds,78C @16:11:20.56 Polling Can every 2:00 @16:11:21.10 PuckHeater.configure :default #ambient 27.6C @16:11:21.16 PuckHeater.hold 10:00,77.4C @16:21:20.02 Lysis complete. Syringe pressure @13.9psi @16:21:20.05 Archive syringe pumping up to 5 strokes to reach 40psi @16:21:20.06 Archive stroke #1 of 5 @16:21:20.14 Archive.in :pressurize @16:21:20.21 Archive.seek :pressurized @16:21:32.72 13.7psi after pressurize @16:21:38.36 13.6psi after pressurize (stable) @16:21:38.38 Archive.in :compress @16:21:38.45 Archive.seek :empty @16:21:50.40 Reached 40.4 of 40.0psi after pumping 1672uL (0.97 strokes) @16:21:50.42 Archive.in :default @16:21:50.48 Archive.hold @16:21:50.59 delay 2 seconds @16:21:52.63 Archive.in :fast @16:21:53.23 Archive.seek :full @16:22:00.22 Lysis1.seek 138uL @16:22:14.08 delay 1 second @16:22:15.12 Archive.seek :full @16:22:15.18 Lysis1.seek :empty @16:22:29.12 Handoff.select :blocked @16:22:29.25 Initial air pressure @41.5psi @16:22:29.36 Lysis1.in :extract @16:22:29.48 Lysis1.seek 600uL,nil @16:22:35.06 Archive syringe pumping up to 6 strokes to reach 25psi @16:22:35.11 Archive stroke #1 of 6 @16:22:35.28 Archive.in :default @16:22:35.35 Archive.hold @16:22:35.53 Initial 600uL lysate @15.3psi @16:22:35.54 Handoff.select :blocked @16:22:35.64 Initial air pressure @15.3psi @16:22:35.74 Lysis1.seek 825uL,nil @16:22:35.84 Archive syringe pumping up to 6 strokes to reach 17psi @16:22:35.85 Archive stroke #1 of 6 @16:22:36.58 Archive.in :pressurize @16:22:36.66 Archive.seek :pressurized @16:22:49.26 15.3psi after pressurize @16:22:56.68 Archive.in :default @16:22:56.76 Archive.hold @16:22:57.09 Recovered 825uL lysate @13.1psi @16:22:57.11 Air.in :halfSpeed @16:22:57.17 Air.seek :fullVent @16:22:59.40 Puck Top Vented, bottom @14.0psi @16:22:59.41 Handoff.select :waste @16:22:59.54 delay 5 seconds @16:23:04.62 Handoff open to waste, bottom @0.5psi @16:23:04.64 Air.in :default @16:23:04.71 Air.seek :evac @16:23:06.12 Lysis2.in :DAmix @16:23:06.29 Lysis1.in :DAmix @16:23:06.94 Lysis2.seek 700uL @16:23:06.98 Lysis1.seek 575uL @16:23:42.08 delay 5 seconds @16:23:47.10 Handoff.select SPR @16:23:47.23 SPR.command "start 2 14" @16:23:48.31 SPR stabilized @16:23:48.47 delay 10 seconds @16:23:58.49 SPR.command "start 2 0" @16:24:01.68 Waiting up to 11:00 for SPR to enter state 1 @16:25:59.35 SPR.command "hold_on" @16:26:01.26 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:26:01.88 Lysis2.seek 400uL @16:26:01.93 Lysis1.seek 275uL @16:26:43.93 delay 15 seconds @16:26:58.95 SPR.command "hold_off" @16:27:02.32 Waiting up to 38:00 for SPR to enter state 7 @16:41:21.56 SPRsummary:143RIU,4e+09RIU,87.4RIU,118RIU,none,high,high after 2906s @16:52:52.00 SPR analysis completed @16:52:59.55 Ended log spr23aug1315h.spr.bz2 @16:52:59.57 SPR.command "stats_off" @16:53:00.18 Handoff.select :close @16:53:00.33 Gate.power :heat,:OFF @16:53:00.35 Gate.power :cartridge,:OFF @16:53:00.43 PinPort.in :unlocking @16:53:00.50 PinPort.seek :clear @16:53:02.42 Carousel.in :default @16:53:02.55 Carousel.home.seek 32 @16:53:02.89 Plunge.seek :locked @16:53:03.79 Archive.in :unlocking @16:53:03.94 Archive.seek :clear @16:53:04.08 Air.in :unlocking @16:53:04.19 Air.seek :clear @16:53:04.24 Lysis1.in :unlocking @16:53:04.43 Lysis1.seek :clear @16:53:04.54 Lysis2.in :unlocking @16:53:05.11 Lysis2.seek :clear @16:53:08.92 Gate.power :motor,:OFF @16:53:08.94 Gate.power :core,:OFF @16:53:08.95 Gate.power :analytic1,:OFF @16:53:08.96 Gate.power :analytic2,:OFF @16:53:08.98 Gate.power :main,:OFF @16:53:08.99 Safely stopped and ready to power off @20:07:01.63 Session Begun @20:07:05.70 -> clients [ESP["ben"]] @20:07:15.30 -> exit @20:07:15.33 Session Ended @20:10:28.38 Session Begun @20:10:41.06 -> Power {analytic1: false, analytic2: false, cartridge: false, core: false, heat: false, main: false, motor: false, sampler: false} @20:14:36.90 -> exit @20:14:36.93 Session Ended @20:48:51.56 Session Begun @20:48:59.96 -> Log.uploadCmd @20:49:08.27 -> showlog 20 @20:49:26.16 -> clients [ESP["brent"]] @20:49:37.71 -> ESP.main.exit # @20:49:37.81 ThreadError in brent -- killed thread @20:49:37.88 Session Ended @20:50:22.48PDT13-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @20:50:24.06 >BREAK< ESP3 Gateway boot v1.43 for core bus @20:50:24.09 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @20:50:27.49 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @20:50:28.10 Sea pressure (Abs) tare is 6% of full scale @20:50:29.48 SyntaxError in MAIN -- /home/esp/esp2/mission/phasecfg.rb:23: syntax error, unexpected tOP_ASGN wardens = |= 'kyle.beadle@noaa.gov','reaga... ^ /home/esp/esp2/mission/phasecfg.rb:23: syntax error, unexpected ',', expecting $end ...s = |= 'kyle.beadle@noaa.gov','reagan.errera@noaa.gov','benj... ^ in /home/esp/esp2/mission/phasecfg.rb @20:50:39.65 Polling Can every 2:00 @20:50:39.69 Waiting for espclients... @20:50:40.45 Session Begun @20:50:50.07 -> Log.uploadCmd @20:51:00.59 -> Email Email @20:51:14.27 -> Email.recipients @20:51:14.29 NoMethodError in brent -- undefined method `recipients' for Email:Class @20:52:37.14 -> GoodNews.recipients @20:52:37.17 NameError in brent -- uninitialized constant GoodNews @20:52:53.65 -> quit @20:52:53.67 Session Ended @20:53:20.82 Session Begun @20:53:32.30 -> showlog 30 @20:53:49.78 -> MainThread.exit # @20:53:49.91 Session Ended @20:54:52.25PDT13-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @20:54:53.82 >BREAK< ESP3 Gateway boot v1.43 for core bus @20:54:53.85 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @20:54:57.14 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @20:54:57.73 Sea pressure (Abs) tare is 6% of full scale @20:54:58.90 SyntaxError in MAIN -- /home/esp/esp2/mission/phasecfg.rb:23: syntax error, unexpected ',', expecting $end ...ens |= 'kyle.beadle@noaa.gov','reagan.errera@noaa.gov','benj... ^ in /home/esp/esp2/mission/phasecfg.rb @20:55:08.52 Polling Can every 2:00 @20:55:08.57 Waiting for espclients... @20:55:09.22 Session Begun @20:55:19.14 -> GoodNews.recipients @20:55:19.18 NameError in brent -- uninitialized constant GoodNews @20:55:21.06 -> GoodNews.recipient @20:55:21.10 NameError in brent -- uninitialized constant GoodNews @20:55:28.38 -> showlog 30 @20:55:40.19 -> exit @20:55:40.22 Session Ended @20:55:42.65 Session Begun @20:55:53.42 -> ESP.main.exit # @20:55:53.54 ThreadError in brent -- killed thread @20:55:53.60 Session Ended @20:56:30.71PDT13-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @20:56:32.37 >BREAK< ESP3 Gateway boot v1.43 for core bus @20:56:32.40 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @20:56:35.84 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @20:56:36.43 Sea pressure (Abs) tare is 6% of full scale @20:56:47.51 Polling Can every 2:00 @20:56:47.56 Waiting for espclients... @20:56:59.25 Session Begun @20:57:05.47 -> GoodNews.recipients ["brent", "nadia", "preston", "methane", "kyle.beadle@noaa.gov", "reagan.errera@noaa.gov", "benjamin.downing@noaa.gov"] @20:57:31.95 -> Log.uploadCmd upsync @20:58:32.74 -> GoodNews.mail "You will get status emails from ESPhonu" Email @20:58:32.81 GoodNews.email "You will get status emails from ESPhonu" @20:58:38.97 upsync @21:03:26.59 -> exit @21:03:26.62 Session Ended @04:44:35.28PDT14-Aug-23 Session Begun @04:44:38.01 -> can Can@04:44:38, 27.0C, 48% humidity, 14.2psia, 14.870V, 0.088A, 14.591Ah, 1.31W @04:45:03.09 -> exit @04:45:03.11 Session Ended @05:32:31.92 Session Begun @05:33:45.88 -> ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @05:33:45.94 Gate.power :sampler,:OFF,limit to 3500mA @05:33:45.96 Gate.power :main,:ON,limit to 5000mA @05:33:46.00 Gate.power :analytic1,:ON,limit to 5000mA @05:33:46.03 Gate.power :analytic2,:ON,limit to 5000mA @05:33:46.22 Powering core bus @05:33:48.48 SPR Module 1, FW Rev. B, FPGA Rev. C @05:33:49.00 Archive.configure :default @05:33:49.16 Air.configure :default @05:33:49.25 Lysis1.configure :default @05:33:49.34 Lysis2.configure :default @05:33:49.46 Plunge.configure :default @05:33:50.10 SamplePump.configure :default @05:33:50.22 Toroid.configure :default @05:33:50.34 Carousel.configure :default @05:33:50.46 Pressure.configure :default @05:33:50.58 ExtraPump.configure :default @05:33:50.68 PinPort.configure :default @05:33:50.76 All elf controllers running firmware version 4.84 @05:33:50.80 Gate.power :motor,:ON,limit to 5000mA @05:33:51.12 Archive.home.jog 0 @05:33:51.81 PinPort.home.jog 0 @05:33:51.86 Carousel.home.jog 0 @05:33:51.90 Toroid.home.jog 0 @05:33:51.94 Lysis2.home.jog 0 @05:33:51.98 Lysis1.home.jog 0 @05:33:52.07 Air.home.jog 0 @05:33:52.14 Plunge.home.jog 0 @05:33:52.94 Archive.in :homing @05:33:53.32 Archive.jog 1392 @05:33:53.40 Toroid.in :homing @05:33:53.44 Lysis2.in :homing @05:33:53.48 Air.in :homing @05:33:53.54 Plunge.in :homing @05:33:53.60 Lysis1.in :homing @05:33:53.68 PinPort.in :homing @05:33:54.01 Lysis2.jog 1391 @05:33:54.07 Toroid.jog 594 @05:33:54.11 Air.jog 1370 @05:33:54.17 Lysis1.jog 1391 @05:33:54.21 Plunge.jog 1572 @05:33:54.26 PinPort.jog -107 @05:33:54.77 PinPort.setRawPosition! 0 @05:33:54.84 PinPort.seek :home @05:33:54.97 Archive.setRawPosition! 50 @05:33:55.00 PinPort.in :default @05:33:55.17 Archive.seek :home @05:33:55.47 Lysis2.setRawPosition! 50 @05:33:55.55 Lysis2.seek :home @05:33:55.73 Lysis1.setRawPosition! 50 @05:33:55.75 Air.setRawPosition! 50 @05:33:55.78 Archive.in :default @05:33:55.96 Lysis1.seek :home @05:33:56.03 Air.seek :home @05:33:56.22 Lysis2.in :default @05:33:56.27 Plunge.setRawPosition! 50 @05:33:56.43 Plunge.seek :home @05:33:56.56 Lysis1.in :default @05:33:56.59 Air.in :default @05:33:56.64 Toroid.setRawPosition! 0 @05:33:56.84 Toroid.seek :home @05:33:56.98 Plunge.in :default @05:33:57.02 Toroid.in :default @05:33:57.44 Toroid.seek :clear @05:33:59.08 Carousel.in :default @05:33:59.24 Carousel.home.seek 32 @05:34:00.66 SPR.command "read_sequences" @05:34:00.73 SPR.command "stats_on" @05:34:00.80 delay 2 seconds @05:34:01.60 SPR.start /var/log/esp/spr23aug1405h.spr.bz2 @05:34:02.82 SPR.command "start 3 0" @05:34:03.49 delay 15:00 @05:49:03.51 SPR.command "start 2 14" @05:49:10.48 Ended log spr23aug1405h.spr.bz2 @05:49:10.50 SPR.command "stats_off" @05:49:10.61 delay 10 seconds @05:49:20.82 Carousel.in :default @05:49:20.98 Carousel.home.seek 32 @05:49:21.37 Lysis1.in :fast @05:49:21.47 Lysis1.seek :locked @05:49:24.00 Plunge.seek :locked @05:49:25.40 Lysis1.in :unlocking @05:49:25.58 Lysis1.seek :clear @05:49:27.14 Gate.power :motor,:OFF @05:49:27.17 Gate.power :core,:OFF @05:49:27.21 Gate.power :analytic1,:OFF @05:49:27.24 Gate.power :analytic2,:OFF @05:49:27.27 Gate.power :main,:OFF @05:49:27.30 Safely stopped and ready to power off :STOPPED @05:50:38.50 -> can Can@05:50:38, 27.3C, 47% humidity, 14.2psia, 14.872V, 0.088A, 14.785Ah, 1.31W @05:54:44.15 -> exit @05:54:44.18 Session Ended @07:28:22.76 Session Begun @07:28:29.76 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @07:30:01.24 -> Can Can@07:28:40, 25.1C, 47% humidity, 14.2psia, 14.885V, 0.081A, 14.917Ah, 1.20W @07:58:11.57 -> clients [ESP["ben"]] @07:59:32.21 -> Can Can@07:58:35, 24.7C, 47% humidity, 14.2psia, 14.884V, 0.081A, 14.958Ah, 1.20W @08:00:44.00 -> startDoublet # @08:00:44.24 Gate.power :sampler,:OFF,limit to 3500mA @08:00:44.26 Gate.power :main,:ON,limit to 5000mA @08:00:44.30 Gate.power :analytic1,:ON,limit to 5000mA @08:00:44.34 Gate.power :analytic2,:ON,limit to 5000mA @08:00:44.43 Powering core bus @08:00:46.78 SPR Module 1, FW Rev. B, FPGA Rev. C @08:00:47.74 Archive.configure :default @08:00:47.86 Air.configure :default @08:00:47.95 Lysis1.configure :default @08:00:48.04 Lysis2.configure :default @08:00:48.16 Plunge.configure :default @08:00:48.28 SamplePump.configure :default @08:00:48.40 Toroid.configure :default @08:00:48.52 Carousel.configure :default @08:00:48.64 Pressure.configure :default @08:00:48.77 ExtraPump.configure :default @08:00:48.85 PinPort.configure :default @08:00:48.94 All elf controllers running firmware version 4.84 @08:00:48.97 Gate.power :motor,:ON,limit to 5000mA @08:00:49.26 Archive.home.jog 0 @08:00:50.06 PinPort.home.jog 0 @08:00:50.09 Toroid.home.jog 0 @08:00:50.19 Plunge.home.jog 0 @08:00:50.22 Lysis1.home.jog 0 @08:00:50.26 Air.home.jog 0 @08:00:50.31 Carousel.home.jog 0 @08:00:50.36 Lysis2.home.jog 0 @08:00:51.19 Archive.in :homing @08:00:51.52 Archive.jog 1382 @08:00:51.77 Plunge.in :homing @08:00:51.83 PinPort.in :homing @08:00:51.88 Lysis2.in :homing @08:00:51.94 Lysis1.in :homing @08:00:52.01 Toroid.in :homing @08:00:52.05 Air.in :homing @08:00:52.35 PinPort.jog -107 @08:00:52.39 Plunge.jog 1568 @08:00:52.62 Toroid.jog 595 @08:00:52.67 Lysis1.jog 1383 @08:00:52.73 Air.jog 1359 @08:00:52.79 Lysis2.jog 1390 @08:00:53.13 PinPort.setRawPosition! 0 @08:00:53.15 Archive.setRawPosition! 50 @08:00:53.29 PinPort.seek :home @08:00:53.32 Archive.seek :home @08:00:53.55 PinPort.in :default @08:00:53.70 Archive.in :default @08:00:53.84 Plunge.setRawPosition! 50 @08:00:53.91 Plunge.seek :home @08:00:54.09 Air.setRawPosition! 50 @08:00:54.13 Lysis1.setRawPosition! 50 @08:00:54.29 Lysis1.seek :home @08:00:54.30 Air.seek :home @08:00:54.53 Plunge.in :default @08:00:54.55 Lysis2.setRawPosition! 50 @08:00:54.69 Lysis2.seek :home @08:00:54.87 Lysis1.in :default @08:00:54.89 Air.in :default @08:00:55.12 Toroid.setRawPosition! 0 @08:00:55.16 Lysis2.in :default @08:00:55.30 Toroid.seek :home @08:00:55.45 Toroid.in :default @08:00:55.62 Toroid.seek :clear @08:00:57.26 Carousel.in :default @08:00:57.42 Carousel.home.seek 32 @08:00:58.22 Selecting Cartridge 19 @08:00:58.57 Carousel.seek 19 @08:01:01.59 Lysis1.in :fast @08:01:01.69 Lysis1.seek :locked @08:01:04.34 Plunge.seek :locked @08:01:04.54 Air.in :fast @08:01:04.64 Air.seek :locked @08:01:06.24 Carousel.reconfigure :locked @08:01:06.43 Powering cartridge bus @08:01:09.11 Gate.setIR 1750,5000,0,6 @08:01:09.24 Gate.setIR 0,5000,0,6 @08:01:09.34 Gate.power :cartridge,:OFF @08:01:09.90 Mounted archive Cartridge @08:01:09.99 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 19" @08:01:10.37 Preparing to filter cartridge 19 @08:01:10.70 Gate.power :sampler,:ON @08:01:10.85 Priming sample loop w/150ml, bypass w/50ml @08:01:10.87 SamplePump.setPosition! 0ml @08:01:10.97 Bypass.open @08:01:12.61 Intake.open @08:01:13.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:01:16.07 Vacuum tare is 56% of full scale @08:01:16.23 upsync @08:01:16.35 Pressure.in :default @08:01:16.44 Pressure.hold @08:01:16.72 Waiting for external sampler to finish priming... @08:01:16.73 delayUntil "08:04:40.72" @08:04:40.77 SamplePump.in :prime @08:04:40.88 SamplePump.seek 50ml,2:53 @08:05:41.91 Pressure.stop @08:05:42.00 SamplePump.setPosition! 0ml @08:05:42.08 Exhaust.open @08:05:43.58 Bypass.close @08:05:45.76 Pressure.in :default @08:05:45.86 Pressure.hold @08:05:46.20 SamplePump.in :prime @08:05:46.30 SamplePump.seek 150ml,7:39 @08:08:16.85 -> Thread Thread @08:08:23.73 -> Power {analytic1: true, analytic2: true, cartridge: false, core: true, heat: false, main: true, motor: true, sampler: true} @08:08:48.63 Pressure.stop @08:08:48.74 Bypass.open @08:08:49.54 Intake.close @08:08:50.39 Toroid.in :hold @08:08:50.49 Toroid.seek :crack @08:08:52.09 Toroid.seek :cartridge @08:08:53.50 Toroid.in :default @08:08:53.60 Toroid.seek :clear @08:08:55.65 Pressurizing cartridge to 26psi above 14.1psia @08:08:55.68 SamplePump.setPosition! 0ml @08:08:55.76 Intake.open @08:08:56.45 Bypass.close @08:08:57.20 Exhaust.close @08:08:58.04 Pressure.in :default @08:08:58.13 Pressure.hold @08:08:58.39 SamplePump.in :charge @08:08:58.49 SamplePump.seek 2.3ml,42 seconds @08:09:07.31 Pressure.stop @08:09:07.40 Intake.close @08:09:08.16 Pressurized cartridge with 0.345ml to 26.1psi @08:09:08.18 Isolated @34.4psia (20.3psi) @08:09:08.22 Verifying cartridge holds at least 0.65..0.85 of 34.4psia @08:09:08.28 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:09:12.88 Cartridge pressure fell 1% to 34.2psia @08:09:13.04 Sampling 1000.0ml @08:09:13.06 SamplePump.setPosition! 0ml @08:09:13.15 Exhaust.open @08:09:13.88 Intake.open @08:09:14.77 Pressure.in :default @08:09:14.87 Pressure.hold @08:09:15.07 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:09:15.30 SamplePump.in :sample @08:09:15.41 SamplePump.seek 1000ml,1:23:51 @08:09:18.42 Cartridge 19 filtering 1/1000ml at 0.59ml/s with 23.2psi vacuum @08:10:00.41 -> help 'Tthread' @08:10:00.55 NoMethodError in ben -- undefined method `help' for CmdServer::Client:Class @08:10:18.42 Cartridge 19 filtering 33/1000ml at 0.52ml/s with 30.7psi vacuum @08:11:18.44 Cartridge 19 filtering 61/1000ml at 0.44ml/s with 17.6psi vacuum @08:11:47.74 -> help 'Thread @08:11:47.76 SyntaxError in ben -- compile error ben:9: unterminated string meets end of file @08:11:57.17 -> help 'Thread' @08:11:57.21 NoMethodError in ben -- undefined method `help' for CmdServer::Client:Class @08:12:18.43 Cartridge 19 filtering 88/1000ml at 0.46ml/s with 19.7psi vacuum @08:13:18.43 Cartridge 19 filtering 114/1000ml at 0.43ml/s with 29.3psi vacuum @08:14:18.43 Cartridge 19 filtering 138/1000ml at 0.39ml/s with 30.3psi vacuum @08:15:18.43 Cartridge 19 filtering 161/1000ml at 0.38ml/s with 14.8psi vacuum @08:16:18.43 Cartridge 19 filtering 183/1000ml at 0.29ml/s with 28.5psi vacuum @08:17:18.43 Cartridge 19 filtering 203/1000ml at 0.27ml/s with 24.1psi vacuum @08:18:18.43 Cartridge 19 filtering 222/1000ml at 0.32ml/s with 14.4psi vacuum @08:19:18.43 Cartridge 19 filtering 239/1000ml at 0.33ml/s with 28.8psi vacuum @08:19:43.33 -> Can Can@08:18:31, 25.1C, 47% humidity, 14.2psia, 14.823V, 0.810A, 15.183Ah, 12.01W @08:20:18.43 Cartridge 19 filtering 255/1000ml at 0.26ml/s with 27.4psi vacuum @08:21:18.43 Cartridge 19 filtering 270/1000ml at 0.20ml/s with 23.0psi vacuum @08:22:18.43 Cartridge 19 filtering 284/1000ml at 0.21ml/s with 28.5psi vacuum @08:22:55.46 Flow averaged 0.36ml/s over 13:41 @08:22:55.53 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @08:22:56.13 Relieved excess vacuum in .7 seconds @08:22:56.19 SamplePump.seek 1000ml,59:30 @08:23:18.43 Cartridge 19 filtering 297/1000ml at 0.24ml/s with 24.2psi vacuum @08:23:20.45 Flow averaged 0.22ml/s over 25 seconds @08:23:20.52 Waiting up to 11 seconds for vacuum to reduce from 27.4 to 18.0psi @08:23:21.12 Relieved excess vacuum in .7 seconds @08:23:21.18 SamplePump.seek 1000ml,59:04 @08:23:32.93 Flow averaged 0.21ml/s over 12 seconds @08:23:33.04 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @08:23:33.65 Relieved excess vacuum in .7 seconds @08:23:33.69 SamplePump.seek 1000ml,58:51 @08:23:45.42 Flow averaged 0.21ml/s over 12 seconds @08:23:45.49 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @08:23:46.09 Relieved excess vacuum in .7 seconds @08:23:46.15 SamplePump.seek 1000ml,58:39 @08:23:53.65 Flow estimate reduced to 0.17ml/s @08:23:53.68 Intake.close @08:23:53.77 Exhaust.close @08:23:54.10 Toroid.in :hold @08:23:54.21 Toroid.seek :bypass @08:23:55.89 Toroid.in :default @08:23:56.00 Toroid.seek :clear @08:23:57.64 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:23:57.67 Pressure.stop @08:23:57.95 Sampled 303.9ml @08:23:58.95 GoodNews.email "After filtering 303.9ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 19" @08:23:59.64 Plunge.seek :reagent @08:24:00.50 Sample loop closed. Ready to Deliver Reagents @08:24:00.53 Archive.seek :sealed @08:24:01.11 Gate.power :sampler,:OFF @08:24:04.52 Archive.seek :evac @08:24:04.77 upsync @08:24:18.61 delay 1:00 @08:25:18.65 Archive.seek :clear @08:25:36.17 RNAlater soak for 10:00 @08:35:36.21 Archive.seek :tVent @08:35:38.69 delay 1 second @08:35:39.72 Archive.seek :evac @08:35:55.27 delay 10 seconds @08:36:05.32 Archive.seek :tVent @08:36:20.88 delay 1 second @08:36:21.91 Archive.seek :evac @08:36:37.45 delay 10 seconds @08:36:47.48 Sample preserved and evacuated @08:36:47.65 Selecting Cartridge 18 @08:36:47.92 Carousel.in :default @08:36:48.09 Carousel.home.seek 19 @08:36:48.49 Archive.in :unlocking @08:36:48.67 Archive.seek :clear @08:36:48.74 Air.in :unlocking @08:36:48.98 Air.seek :clear @08:36:53.36 Lysis1.in :unlocking @08:36:53.54 Lysis1.seek :clear @08:36:53.60 Plunge.in :unlocking @08:36:53.76 Plunge.seek :clear @08:36:55.19 Carousel.seek 18 @08:36:55.78 Lysis1.in :fast @08:36:55.87 Lysis1.seek :locked @08:36:58.53 Plunge.in :default @08:36:58.65 Plunge.seek :locked @08:36:59.30 Air.in :fast @08:36:59.49 Air.seek :locked @08:37:01.08 Carousel.reconfigure :locked @08:37:01.30 Powering cartridge bus @08:37:03.96 Gate.setIR 1750,5000,0,6 @08:37:04.09 Gate.setIR 0,5000,0,6 @08:37:04.19 PuckHeater.configure :default @08:37:04.28 Gate.power :heat,:ON,limit to 5000mA @08:37:05.07 Mounted lysis Cartridge board#Q05057 @08:37:05.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:37:05.19 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 18" @08:37:05.57 Preparing to filter cartridge 18 @08:37:05.91 Gate.power :sampler,:ON @08:37:06.05 Priming sample loop w/150ml, bypass w/50ml @08:37:06.09 SamplePump.setPosition! 0ml @08:37:06.19 Bypass.open @08:37:07.79 Intake.open @08:37:08.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:37:11.18 Vacuum tare is 56% of full scale @08:37:11.40 Pressure.in :default @08:37:11.51 Pressure.hold @08:37:11.76 upsync @08:37:11.85 Waiting for external sampler to finish priming... @08:37:11.87 delayUntil "08:40:35.94" @08:40:35.99 SamplePump.in :prime @08:40:36.10 SamplePump.seek 50ml,2:53 @08:41:37.13 Pressure.stop @08:41:37.22 SamplePump.setPosition! 0ml @08:41:37.31 Exhaust.open @08:41:38.76 Bypass.close @08:41:40.36 Pressure.in :default @08:41:40.45 Pressure.hold @08:41:40.79 SamplePump.in :prime @08:41:40.90 SamplePump.seek 150ml,7:39 @08:44:43.43 Pressure.stop @08:44:44.13 Bypass.open @08:44:44.85 Intake.close @08:44:45.71 Toroid.in :hold @08:44:45.80 Toroid.seek :crack @08:44:47.38 Toroid.seek :cartridge @08:44:48.51 Toroid.in :default @08:44:48.61 Toroid.seek :clear @08:44:50.65 Pressurizing cartridge to 26psi above 14.1psia @08:44:50.67 SamplePump.setPosition! 0ml @08:44:50.76 Intake.open @08:44:51.45 Bypass.close @08:44:52.18 Exhaust.close @08:44:53.07 Pressure.in :default @08:44:53.16 Pressure.hold @08:44:53.42 SamplePump.in :charge @08:44:53.52 SamplePump.seek 2.3ml,42 seconds @08:45:06.59 Pressure.stop @08:45:06.69 Intake.close @08:45:07.47 Pressurized cartridge with 0.770ml to 26.3psi @08:45:07.49 Isolated @34.7psia (20.7psi) @08:45:07.52 Verifying cartridge holds at least 0.65..0.85 of 34.7psia @08:45:07.58 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:45:12.26 Sampling 50.0ml @08:45:12.28 SamplePump.setPosition! 0ml @08:45:12.38 Exhaust.open @08:45:13.11 Intake.open @08:45:13.98 Pressure.in :default @08:45:14.07 Pressure.hold @08:45:14.24 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:45:14.45 SamplePump.in :sample @08:45:14.55 SamplePump.seek 50ml,4:41 @08:45:17.58 Cartridge 18 filtering 1/50ml at 0.56ml/s with 19.8psi vacuum @08:46:17.59 Cartridge 18 filtering 32/50ml at 0.49ml/s with 21.7psi vacuum @08:46:54.89 Pressure.stop @08:46:55.15 Intake.close @08:46:55.19 Exhaust.close @08:46:55.53 Toroid.in :hold @08:46:55.64 Toroid.seek :bypass @08:46:57.30 Toroid.in :default @08:46:57.40 Toroid.seek :clear @08:46:59.60 Sampled 50.0ml @08:47:00.10 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 18" @08:47:01.84 SPR.command "read_sequences" @08:47:02.66 SPR.command "stats_on" @08:47:02.85 Gate.power :sampler,:OFF @08:47:02.94 SPR.command "start 0 1" @08:47:03.58 SPR.start /var/log/esp/spr23aug1408h.spr.bz2 @08:47:05.51 Handoff.select :waste @08:47:05.79 upsync @08:47:12.27 Syringe tare is 17% of full scale @08:47:12.30 PinPort.in :locking @08:47:12.42 PinPort.seek :locked @08:47:14.97 Archive.in :fast @08:47:15.07 Lysis2.in :fast @08:47:15.16 Lysis1.seek 1350uL @08:47:15.35 Archive.seek :full @08:47:15.48 Lysis2.seek 950uL @08:47:20.23 Plunge.seek :reagent @08:47:21.21 Evacuating puck with filter bubble point of 50psi @08:47:21.24 PinPort.seek :locked @08:47:21.38 Handoff.select :waste @08:47:28.00 Air syringe pumping up to 1 strokes to reach 8psi @08:47:28.01 Air stroke #1 of 1 @08:47:28.16 Air.in :pressurize @08:47:28.25 Air.seek :pressurized @08:47:34.03 1.2psi after pressurize @08:47:34.06 Air.in :compress @08:47:34.16 Air.seek :evac @08:47:37.25 0.9psi after compress @08:47:47.87 0.1psi after compress (stable) @08:47:47.89 Air.in :default @08:47:48.00 Air.hold @08:47:48.25 at 0.0psi Opened to waste @08:47:48.26 Handoff.select :blocked @08:47:48.44 Archive syringe pumping up to 8 strokes to reach 50psi @08:47:48.46 Archive stroke #1 of 8 @08:47:48.62 Archive.in :pressurize @08:47:48.72 Archive.seek :pressurized @08:48:01.67 6.4psi after pressurize @08:48:07.39 6.4psi after pressurize (stable) @08:48:07.44 Archive.in :compress @08:48:07.57 Archive.seek :empty @08:48:22.24 20.2psi after compress @08:48:35.89 24.9psi after compress (stable) @08:48:35.92 Archive.in :gulp @08:48:36.03 Archive.seek :topVent @08:48:42.33 26.2psi after gulp @08:48:47.93 26.1psi after gulp (stable) @08:48:47.95 Archive stroke #2 of 8 @08:48:48.09 Archive.in :pressurize @08:48:48.24 Archive.seek :pressurized @08:49:00.77 25.2psi after pressurize @08:49:06.37 25.4psi after pressurize (stable) @08:49:06.39 Archive.in :compress @08:49:06.50 Archive.seek :empty @08:49:21.15 37.7psi after compress @08:49:34.25 41.9psi after compress (stable) @08:49:34.28 Archive.in :gulp @08:49:34.41 Archive.seek :topVent @08:49:40.72 43.3psi after gulp @08:49:46.32 43.3psi after gulp (stable) @08:49:46.34 Archive stroke #3 of 8 @08:49:46.48 Archive.in :pressurize @08:49:46.57 Archive.seek :pressurized @08:49:59.18 42.4psi after pressurize @08:50:04.82 42.6psi after pressurize (stable) @08:50:04.85 Archive.in :compress @08:50:04.97 Archive.seek :empty @08:50:16.87 Reached 50.2 of 50.0psi after pumping 5129uL (2.96 strokes) @08:50:16.89 Archive.in :default @08:50:17.04 Archive.hold @08:50:17.19 Handoff.select :waste @08:50:28.92 delay 1:00 @08:51:28.94 Handoff.select :blocked @08:51:51.72 Handoff.select :waste @08:51:51.87 0.6psi at evacEnd @08:51:51.88 Handoff.select :blocked @08:51:52.05 Archive.in :fast @08:51:52.14 Archive.seek :topVent @08:52:05.65 Stable @0.7psi after Handoff blocked @08:52:05.68 Archive syringe pumping up to 8 strokes to reach 31psi @08:52:05.72 Archive stroke #1 of 8 @08:52:05.90 Archive.in :pressurize @08:52:06.00 Archive.seek :pressurized @08:52:18.54 0.8psi after pressurize @08:52:18.57 Archive.in :compress @08:52:18.68 Archive.seek :empty @08:52:33.35 14.0psi after compress @08:52:43.95 15.5psi after compress (stable) @08:52:43.98 Archive.in :gulp @08:52:44.10 Archive.seek :topVent @08:52:50.39 15.8psi after gulp @08:52:56.03 15.8psi after gulp (stable) @08:52:56.05 Archive stroke #2 of 8 @08:52:56.20 Archive.in :pressurize @08:52:56.29 Archive.seek :pressurized @08:53:08.83 15.6psi after pressurize @08:53:08.85 Archive.in :compress @08:53:09.05 Archive.seek :empty @08:53:23.77 28.2psi after compress @08:53:34.37 29.5psi after compress (stable) @08:53:34.40 Archive.in :gulp @08:53:34.51 Archive.seek :topVent @08:53:40.78 30.0psi after gulp @08:53:46.38 30.0psi after gulp (stable) @08:53:46.39 Archive stroke #3 of 8 @08:53:46.54 Archive.in :pressurize @08:53:46.64 Archive.seek :pressurized @08:53:59.18 29.7psi after pressurize @08:53:59.21 Archive.in :compress @08:53:59.36 Archive.seek :empty @08:54:07.53 Reached 30.9 of 30.7psi after pumping 5040uL (2.91 strokes) @08:54:07.55 Archive.in :default @08:54:07.67 Archive.hold @08:54:07.78 Handoff.select :waste @08:54:20.55 delay 1:00 @08:55:20.58 Handoff.select :blocked @08:55:43.98 Handoff.select :waste @08:55:44.12 0.5psi at evacEnd @08:55:44.15 Air.seek :fullVent @08:55:45.61 Archive.in :fast @08:55:45.71 Archive.seek :topVent @08:55:52.32 Seawater evacuated, Pressure above/below filter released. @08:55:52.45 Lysis1.in :slow @08:55:52.54 Lysis1.seek 1050uL @08:56:22.59 delay 5 seconds @08:56:27.62 Handoff.select :blocked @08:56:27.91 Lysis1.seek 450uL @08:57:27.87 delay 3 seconds @08:57:31.44 Air.in mix with 1.40048 parts Lysis1 @08:57:32.20 Air.seek :evac @08:57:32.28 Lysis1.seek 300uL @08:57:47.43 Lysis1.seek :empty @08:58:17.62 Lysis buffer delivered @21.2psi @08:58:17.65 Polling Can every 2:00 @08:58:18.21 PuckHeater.configure :default #ambient 25.6C @08:58:18.30 PuckHeater.seek 79.6C @08:59:20.24 PuckHeater.hold 2:00,79.6C @09:01:20.35 Polling Can every 2:00 @09:01:20.90 PuckHeater.configure :default #ambient 25.6C @09:01:21.04 PuckHeater.hold 30 seconds,78.6C @09:01:51.29 Polling Can every 2:00 @09:01:51.86 PuckHeater.configure :default #ambient 25.6C @09:01:52.47 PuckHeater.hold 30 seconds,78C @09:02:22.70 Polling Can every 2:00 @09:02:23.08 PuckHeater.hold 10:00,77.5C @09:12:22.85 Lysis complete. Syringe pressure @25.6psi @09:12:22.88 Archive syringe pumping up to 5 strokes to reach 40psi @09:12:22.91 Archive stroke #1 of 5 @09:12:23.10 Archive.in :pressurize @09:12:23.22 Archive.seek :pressurized @09:12:35.75 24.9psi after pressurize @09:12:41.77 24.7psi after pressurize (stable) @09:12:41.88 Archive.in :compress @09:12:42.00 Archive.seek :empty @09:12:51.66 Reached 40.7 of 40.0psi after pumping 1621uL (0.94 strokes) @09:12:51.68 Archive.in :default @09:12:51.79 Archive.hold @09:12:51.90 delay 2 seconds @09:12:53.93 Archive.in :fast @09:12:54.02 Archive.seek :full @09:13:00.85 Lysis1.seek 138uL @09:13:14.76 delay 1 second @09:13:15.80 Archive.seek :full @09:13:15.88 Lysis1.seek :empty @09:13:29.85 Handoff.select :blocked @09:13:30.00 Initial air pressure @46.6psi @09:13:30.07 Lysis1.in :extract @09:13:30.26 Lysis1.seek 600uL,nil @09:13:33.17 Archive syringe pumping up to 6 strokes to reach 25psi @09:13:33.19 Archive stroke #1 of 6 @09:13:33.42 Archive.in :pressurize @09:13:33.54 Archive.seek :pressurized @09:13:46.09 13.7psi after pressurize @09:13:51.72 13.7psi after pressurize (stable) @09:13:51.75 Archive.in :compress @09:13:52.47 Archive.seek :empty @09:14:02.20 Archive.in :default @09:14:02.29 Archive.hold @09:14:02.55 Initial 600uL lysate @16.2psi @09:14:02.57 Handoff.select :blocked @09:14:02.73 Initial air pressure @17.4psi @09:14:02.84 Lysis1.seek 825uL,nil @09:14:04.19 Archive syringe pumping up to 6 strokes to reach 17psi @09:14:04.21 Archive stroke #1 of 6 @09:14:04.38 Archive.in :compress @09:14:04.48 Archive.seek :empty @09:14:06.64 Reached 17.1 of 17.0psi after pumping 1669uL (0.96 strokes) @09:14:06.66 Archive.in :default @09:14:06.77 Archive.hold @09:14:07.85 Recovered 825uL lysate @16.3psi @09:14:07.99 Air.in :halfSpeed @09:14:08.17 Air.seek :fullVent @09:14:10.48 Puck Top Vented, bottom @18.6psi @09:14:10.50 Handoff.select :waste @09:14:10.67 delay 5 seconds @09:14:15.76 Handoff open to waste, bottom @0.6psi @09:14:15.79 Air.in :default @09:14:15.90 Air.seek :evac @09:14:17.35 Lysis2.in :DAmix @09:14:17.54 Lysis1.in :DAmix @09:14:18.17 Lysis2.seek 700uL @09:14:18.27 Lysis1.seek 575uL @09:14:53.33 delay 5 seconds @09:14:58.35 Handoff.select SPR @09:14:58.51 SPR.command "start 2 14" @09:15:00.14 SPR stabilized @09:15:00.33 delay 10 seconds @09:15:10.35 SPR.command "start 2 0" @09:15:12.99 Waiting up to 11:00 for SPR to enter state 1 @09:17:10.67 SPR.command "hold_on" @09:17:12.06 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:17:12.74 Lysis2.seek 400uL @09:17:12.79 Lysis1.seek 275uL @09:17:54.87 delay 15 seconds @09:18:09.89 SPR.command "hold_off" @09:18:12.71 Waiting up to 38:00 for SPR to enter state 7 @09:32:32.89 SPRsummary:-17.2RIU,-0.748RIU,68.2RIU,86.2RIU,high,168ng/L,297ng/L after 2883s @09:44:03.32 SPR analysis completed @09:44:10.35 Ended log spr23aug1408h.spr.bz2 @09:44:10.37 SPR.command "stats_off" @09:44:10.69 Handoff.select :close @09:44:10.87 Gate.power :heat,:OFF @09:44:10.89 Gate.power :cartridge,:OFF @09:44:11.05 PinPort.in :unlocking @09:44:11.15 PinPort.seek :clear @09:44:13.09 Carousel.in :default @09:44:13.25 Carousel.home.seek 18 @09:44:13.61 Plunge.seek :locked @09:44:14.52 Archive.in :unlocking @09:44:14.70 Archive.seek :clear @09:44:14.83 Air.in :unlocking @09:44:14.99 Air.seek :clear @09:44:15.12 Lysis1.in :unlocking @09:44:15.37 Lysis1.seek :clear @09:44:15.43 Lysis2.in :unlocking @09:44:15.59 Lysis2.seek :clear @09:44:19.87 Gate.power :motor,:OFF @09:44:19.89 Gate.power :core,:OFF @09:44:19.92 Gate.power :analytic1,:OFF @09:44:19.96 Gate.power :analytic2,:OFF @09:44:19.99 Gate.power :main,:OFF @09:44:20.03 Safely stopped and ready to power off @09:45:59.60 -> exit @09:45:59.63 Session Ended @09:46:09.85 Session Begun @09:47:24.59 -> exit @09:47:24.61 Session Ended @09:47:34.73 Session Begun @09:47:41.05 -> clients [ESP["ben"]] @09:48:22.15 -> ESP.ready!; SPR.start; delay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.command “start 2 14"; SPR.stop; delay 10; Cmd.stop @09:48:22.20 SyntaxError in ben -- compile error ben:2: Invalid char `\342' in expression ben:2: Invalid char `\200' in expression ben:2: Invalid char `\234' in expression ben:2: syntax error, unexpected tINTEGER, expecting $end ...ay 2; SPR.command “start 3 0”; delay ‘15:00’; SPR.co... ^ @09:55:05.60 -> Can Can@09:54:13, 25.2C, 47% humidity, 14.2psia, 14.887V, 0.082A, 16.213Ah, 1.21W @09:57:02.00 -> ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay '15:00'; SPR.command "start 2 14"; SPR.stop; delay 10; Cmd.stop @09:57:02.06 Gate.power :sampler,:OFF,limit to 3500mA @09:57:02.08 Gate.power :main,:ON,limit to 5000mA @09:57:02.11 Gate.power :analytic1,:ON,limit to 5000mA @09:57:02.15 Gate.power :analytic2,:ON,limit to 5000mA @09:57:02.26 Powering core bus @09:57:04.60 SPR Module 1, FW Rev. B, FPGA Rev. C @09:57:05.07 Archive.configure :default @09:57:05.19 Air.configure :default @09:57:05.27 Lysis1.configure :default @09:57:05.36 Lysis2.configure :default @09:57:05.49 Plunge.configure :default @09:57:05.60 SamplePump.configure :default @09:57:05.73 Toroid.configure :default @09:57:05.85 Carousel.configure :default @09:57:05.98 Pressure.configure :default @09:57:06.10 ExtraPump.configure :default @09:57:06.19 PinPort.configure :default @09:57:06.28 All elf controllers running firmware version 4.84 @09:57:06.31 Gate.power :motor,:ON,limit to 5000mA @09:57:06.70 Air.home.jog 0 @09:57:06.94 Archive.home.jog 0 @09:57:07.59 Carousel.home.jog 0 @09:57:07.66 Toroid.home.jog 0 @09:57:07.70 Lysis2.home.jog 0 @09:57:07.73 Lysis1.home.jog 0 @09:57:07.79 Plunge.home.jog 0 @09:57:07.86 PinPort.home.jog 0 @09:57:08.58 Air.in :homing @09:57:08.71 Archive.in :homing @09:57:08.85 Air.jog 1371 @09:57:08.88 Archive.jog 1389 @09:57:09.22 Plunge.in :homing @09:57:09.28 PinPort.in :homing @09:57:09.33 Lysis2.in :homing @09:57:09.40 Toroid.in :homing @09:57:09.44 Lysis1.in :homing @09:57:09.69 Plunge.jog 1577 @09:57:09.76 PinPort.jog -107 @09:57:09.83 Lysis2.jog 1395 @09:57:09.93 Lysis1.jog 1390 @09:57:10.03 Toroid.jog 591 @09:57:10.40 Air.setRawPosition! 50 @09:57:10.42 Archive.setRawPosition! 50 @09:57:10.47 PinPort.setRawPosition! 0 @09:57:10.63 Air.seek :home @09:57:10.68 PinPort.seek :home @09:57:10.72 Archive.seek :home @09:57:11.64 Plunge.setRawPosition! 50 @09:57:11.70 Lysis2.setRawPosition! 50 @09:57:11.74 Air.in :default @09:57:11.78 PinPort.in :default @09:57:11.83 Archive.in :default @09:57:11.88 Lysis1.setRawPosition! 50 @09:57:12.14 Plunge.seek :home @09:57:12.18 Lysis2.seek :home @09:57:12.41 Lysis1.seek :home @09:57:12.67 Toroid.setRawPosition! 0 @09:57:12.69 Plunge.in :default @09:57:12.82 Toroid.seek :home @09:57:13.06 Toroid.in :default @09:57:13.09 Lysis2.in :default @09:57:13.13 Lysis1.in :default @09:57:13.60 Toroid.seek :clear @09:57:15.25 Carousel.in :default @09:57:15.42 Carousel.home.seek 18 @09:57:16.83 SPR.command "read_sequences" @09:57:16.90 SPR.command "stats_on" @09:57:17.71 delay 2 seconds @09:57:18.51 SPR.start /var/log/esp/spr23aug1409h.spr.bz2 @09:57:19.74 SPR.command "start 3 0" @09:57:20.55 delay 15:00 @10:12:19.44 Session Begun @10:12:20.57 SPR.command "start 2 14" @10:12:27.27 Ended log spr23aug1409h.spr.bz2 @10:12:27.32 SPR.command "stats_off" @10:12:27.59 delay 10 seconds @10:12:37.80 Carousel.in :default @10:12:37.97 Carousel.home.seek 18 @10:12:38.82 Lysis1.in :fast @10:12:38.93 Lysis1.seek :locked @10:12:41.50 Plunge.seek :locked @10:12:42.92 Lysis1.in :unlocking @10:12:43.16 Lysis1.seek :clear @10:12:44.72 Gate.power :motor,:OFF @10:12:44.75 Gate.power :core,:OFF @10:12:44.80 Gate.power :analytic1,:OFF @10:12:44.85 Gate.power :analytic2,:OFF @10:12:44.89 Gate.power :main,:OFF @10:12:44.93 Safely stopped and ready to power off @10:12:45.32 -> exit @10:12:45.56 Session Ended :STOPPED @10:43:38.79 -> Power {analytic1: false, analytic2: false, cartridge: false, core: false, heat: false, main: false, motor: false, sampler: false} @11:11:27.46 -> startDoublet # @11:11:27.70 Gate.power :sampler,:OFF,limit to 3500mA @11:11:27.73 Gate.power :main,:ON,limit to 5000mA @11:11:27.76 Gate.power :analytic1,:ON,limit to 5000mA @11:11:27.80 Gate.power :analytic2,:ON,limit to 5000mA @11:11:27.90 Powering core bus @11:11:30.24 SPR Module 1, FW Rev. B, FPGA Rev. C @11:11:30.69 Archive.configure :default @11:11:30.81 Air.configure :default @11:11:30.90 Lysis1.configure :default @11:11:31.00 Lysis2.configure :default @11:11:31.16 Plunge.configure :default @11:11:31.29 SamplePump.configure :default @11:11:31.41 Toroid.configure :default @11:11:31.53 Carousel.configure :default @11:11:31.65 Pressure.configure :default @11:11:31.77 ExtraPump.configure :default @11:11:31.86 PinPort.configure :default @11:11:31.95 All elf controllers running firmware version 4.84 @11:11:31.98 Gate.power :motor,:ON,limit to 5000mA @11:11:32.28 Archive.home.jog 0 @11:11:33.06 PinPort.home.jog 0 @11:11:33.11 Toroid.home.jog 0 @11:11:33.17 Plunge.home.jog 0 @11:11:33.22 Lysis2.home.jog 0 @11:11:33.27 Lysis1.home.jog 0 @11:11:33.32 Air.home.jog 0 @11:11:33.38 Carousel.home.jog 0 @11:11:34.16 Archive.in :homing @11:11:34.57 Archive.jog 1389 @11:11:34.65 Toroid.in :homing @11:11:34.70 Lysis2.in :homing @11:11:34.74 Air.in :homing @11:11:34.82 Plunge.in :homing @11:11:34.87 Lysis1.in :homing @11:11:35.40 PinPort.in :homing @11:11:35.84 Lysis2.jog 1393 @11:11:35.92 Toroid.jog 580 @11:11:35.94 Plunge.jog 1571 @11:11:36.01 Air.jog 1364 @11:11:36.06 PinPort.jog -108 @11:11:36.09 Lysis1.jog 1386 @11:11:36.60 PinPort.setRawPosition! 0 @11:11:36.68 PinPort.seek :home @11:11:36.81 Archive.setRawPosition! 50 @11:11:36.84 PinPort.in :default @11:11:36.97 Archive.seek :home @11:11:37.35 Lysis2.setRawPosition! 50 @11:11:37.37 Archive.in :default @11:11:37.56 Lysis2.seek :home @11:11:37.72 Lysis1.setRawPosition! 50 @11:11:37.75 Air.setRawPosition! 50 @11:11:37.79 Plunge.setRawPosition! 50 @11:11:37.97 Lysis1.seek :home @11:11:38.01 Plunge.seek :home @11:11:38.06 Air.seek :home @11:11:38.33 Toroid.setRawPosition! 0 @11:11:38.39 Lysis2.in :default @11:11:38.53 Toroid.seek :home @11:11:38.72 Toroid.in :default @11:11:38.76 Plunge.in :default @11:11:38.80 Air.in :default @11:11:38.86 Lysis1.in :default @11:11:39.44 Toroid.seek :clear @11:11:41.09 Carousel.in :default @11:11:41.25 Carousel.home.seek 18 @11:11:41.56 Selecting Cartridge 17 @11:11:41.90 Carousel.seek 17 @11:11:42.46 Lysis1.in :fast @11:11:42.57 Lysis1.seek :locked @11:11:45.23 Plunge.seek :locked @11:11:45.41 Air.in :fast @11:11:45.50 Air.seek :locked @11:11:47.10 Carousel.reconfigure :locked @11:11:47.31 Powering cartridge bus @11:11:49.96 Gate.setIR 1750,5000,0,6 @11:11:50.09 Gate.setIR 0,5000,0,6 @11:11:50.19 Gate.power :cartridge,:OFF @11:11:50.76 Mounted archive Cartridge @11:11:50.85 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 17" @11:11:51.79 Preparing to filter cartridge 17 @11:11:52.10 Gate.power :sampler,:ON @11:11:52.25 Priming sample loop w/150ml, bypass w/50ml @11:11:52.28 SamplePump.setPosition! 0ml @11:11:52.36 Bypass.open @11:11:53.93 Intake.open @11:11:54.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:11:57.55 Vacuum tare is 56% of full scale @11:11:57.84 Pressure.in :default @11:11:57.94 Pressure.hold @11:11:58.21 upsync @11:11:58.28 Waiting for external sampler to finish priming... @11:11:58.30 delayUntil "11:15:22.13" @11:14:43.34 Session Begun @11:14:50.73 -> showlog 20 @11:15:04.52 -> def doubleRegen; ESP.ready!; SPR.start; delay 2; SPR.command "start 3 0"; delay 15.minutes; SPR.command "start 2 14"; ensure; SPR.stop; delay 10; Cmd.stop; end :doubleRegen @11:15:20.97 -> exit @11:15:21.00 Session Ended @11:15:22.18 SamplePump.in :prime @11:15:22.30 SamplePump.seek 50ml,2:53 @11:16:23.35 Pressure.stop @11:16:23.43 SamplePump.setPosition! 0ml @11:16:23.51 Exhaust.open @11:16:24.96 Bypass.close @11:16:26.59 Pressure.in :default @11:16:26.68 Pressure.hold @11:16:27.05 SamplePump.in :prime @11:16:27.16 SamplePump.seek 150ml,7:39 @11:19:29.36 Pressure.stop @11:19:29.47 Bypass.open @11:19:30.25 Intake.close @11:19:31.09 Toroid.in :hold @11:19:31.19 Toroid.seek :crack @11:19:32.74 Toroid.seek :cartridge @11:19:33.88 Toroid.in :default @11:19:33.98 Toroid.seek :clear @11:19:36.10 Pressurizing cartridge to 26psi above 14.0psia @11:19:36.12 SamplePump.setPosition! 0ml @11:19:36.21 Intake.open @11:19:36.88 Bypass.close @11:19:37.63 Exhaust.close @11:19:38.51 Pressure.in :default @11:19:38.60 Pressure.hold @11:19:38.87 SamplePump.in :charge @11:19:38.98 SamplePump.seek 2.3ml,42 seconds @11:19:47.97 Pressure.stop @11:19:48.07 Intake.close @11:19:48.84 Pressurized cartridge with 0.358ml to 26.4psi @11:19:48.85 Isolated @35.4psia (21.3psi) @11:19:48.88 Verifying cartridge holds at least 0.65..0.85 of 35.4psia @11:19:48.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:19:53.56 Cartridge pressure fell 2% to 34.7psia @11:19:53.69 Sampling 1000.0ml @11:19:53.72 SamplePump.setPosition! 0ml @11:19:53.81 Exhaust.open @11:19:54.57 Intake.open @11:19:55.43 Pressure.in :default @11:19:55.52 Pressure.hold @11:19:55.70 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:19:55.93 SamplePump.in :sample @11:19:56.04 SamplePump.seek 1000ml,1:23:51 @11:19:59.09 Cartridge 17 filtering 1/1000ml at 0.57ml/s with 29.2psi vacuum @11:20:59.10 Cartridge 17 filtering 31/1000ml at 0.51ml/s with 25.0psi vacuum @11:21:59.10 Cartridge 17 filtering 58/1000ml at 0.47ml/s with 27.3psi vacuum @11:22:59.10 Cartridge 17 filtering 83/1000ml at 0.39ml/s with 31.3psi vacuum @11:23:59.09 Cartridge 17 filtering 105/1000ml at 0.31ml/s with 27.8psi vacuum @11:24:59.10 Cartridge 17 filtering 126/1000ml at 0.37ml/s with 19.7psi vacuum 1.9m deep (17.5psia) @11:25:59.10 Cartridge 17 filtering 144/1000ml at 0.26ml/s with 16.0psi vacuum @11:26:59.10 Cartridge 17 filtering 161/1000ml at 0.30ml/s with 30.3psi vacuum @11:27:59.10 Cartridge 17 filtering 175/1000ml at 0.20ml/s with 21.8psi vacuum @11:28:33.76 Flow averaged 0.35ml/s over 8:38 @11:28:33.83 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @11:28:34.43 Relieved excess vacuum in .7 seconds @11:28:34.49 SamplePump.seek 1000ml,1:08:36 @11:28:56.97 Flow averaged 0.22ml/s over 23 seconds @11:28:57.07 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @11:28:57.69 Relieved excess vacuum in .7 seconds @11:28:57.74 SamplePump.seek 1000ml,1:08:11 @11:28:59.10 Cartridge 17 filtering 188/1000ml at 0.20ml/s with 26.3psi vacuum @11:29:10.65 Flow averaged 0.21ml/s over 13 seconds @11:29:10.73 Waiting up to 13 seconds for vacuum to reduce from 28.1 to 18.0psi @11:29:11.34 Relieved excess vacuum in .7 seconds @11:29:11.38 SamplePump.seek 1000ml,1:07:57 @11:29:20.25 Flow estimate reduced to 0.17ml/s @11:29:20.27 Intake.close @11:29:20.34 Exhaust.close @11:29:20.70 Toroid.in :hold @11:29:20.80 Toroid.seek :bypass @11:29:22.49 Toroid.in :default @11:29:22.60 Toroid.seek :clear @11:29:24.26 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:29:24.28 Pressure.stop @11:29:24.58 Sampled 192.6ml @11:29:25.10 GoodNews.email "After filtering 192.6ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 17" @11:29:25.73 Plunge.seek :reagent @11:29:26.57 Sample loop closed. Ready to Deliver Reagents @11:29:26.60 Archive.seek :sealed @11:29:27.75 Gate.power :sampler,:OFF @11:29:30.62 Archive.seek :evac @11:29:30.76 upsync @11:29:44.71 delay 1:00 @11:30:44.75 Archive.seek :clear @11:31:02.83 RNAlater soak for 10:00 @11:41:02.86 Archive.seek :tVent @11:41:05.34 delay 1 second @11:41:06.37 Archive.seek :evac @11:41:21.91 delay 10 seconds @11:41:31.95 Archive.seek :tVent @11:41:47.50 delay 1 second @11:41:48.52 Archive.seek :evac @11:42:04.08 delay 10 seconds @11:42:14.11 Sample preserved and evacuated @11:42:14.27 Selecting Cartridge 16 @11:42:14.54 Carousel.in :default @11:42:14.70 Carousel.home.seek 17 @11:42:15.13 Archive.in :unlocking @11:42:15.28 Archive.seek :clear @11:42:15.42 Air.in :unlocking @11:42:15.61 Air.seek :clear @11:42:19.81 Lysis1.in :unlocking @11:42:19.99 Lysis1.seek :clear @11:42:20.05 Plunge.in :unlocking @11:42:20.20 Plunge.seek :clear @11:42:21.64 Carousel.seek 16 @11:42:22.22 Lysis1.in :fast @11:42:22.31 Lysis1.seek :locked @11:42:24.94 Plunge.in :default @11:42:25.06 Plunge.seek :locked @11:42:25.25 Air.in :fast @11:42:25.35 Air.seek :locked @11:42:26.94 Carousel.reconfigure :locked @11:42:27.16 Powering cartridge bus @11:42:29.82 Gate.setIR 1750,5000,0,6 @11:42:29.96 Gate.setIR 0,5000,0,6 @11:42:30.05 PuckHeater.configure :default #ambient 25.6C @11:42:30.13 Gate.power :heat,:ON,limit to 5000mA @11:42:30.83 Mounted lysis Cartridge board#Q05080 @11:42:30.89 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:42:30.97 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 16" @11:42:31.40 Preparing to filter cartridge 16 @11:42:31.72 Gate.power :sampler,:ON @11:42:32.38 Priming sample loop w/150ml, bypass w/50ml @11:42:32.40 SamplePump.setPosition! 0ml @11:42:32.49 Bypass.open @11:42:34.10 Intake.open @11:42:34.86 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:42:37.47 Vacuum tare is 56% of full scale @11:42:37.62 upsync @11:42:37.75 Pressure.in :default @11:42:37.84 Pressure.hold @11:42:38.11 Waiting for external sampler to finish priming... @11:42:38.13 delayUntil "11:46:01.74" @11:44:56.33 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1408h.spr.bz2}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @11:46:01.80 SamplePump.in :prime @11:46:01.91 SamplePump.seek 50ml,2:53 @11:47:02.95 Pressure.stop @11:47:03.07 SamplePump.setPosition! 0ml @11:47:03.15 Exhaust.open @11:47:04.62 Bypass.close @11:47:06.22 Pressure.in :default @11:47:06.32 Pressure.hold @11:47:06.66 SamplePump.in :prime @11:47:06.77 SamplePump.seek 150ml,7:39 @11:50:09.33 Pressure.stop @11:50:09.45 Bypass.open @11:50:10.21 Intake.close @11:50:11.07 Toroid.in :hold @11:50:11.17 Toroid.seek :crack @11:50:12.74 Toroid.seek :cartridge @11:50:13.88 Toroid.in :default @11:50:13.98 Toroid.seek :clear @11:50:16.03 Pressurizing cartridge to 26psi above 14.1psia @11:50:16.06 SamplePump.setPosition! 0ml @11:50:16.69 Intake.open @11:50:17.37 Bypass.close @11:50:18.10 Exhaust.close @11:50:18.97 Pressure.in :default @11:50:19.11 Pressure.hold @11:50:19.38 SamplePump.in :charge @11:50:19.48 SamplePump.seek 2.3ml,42 seconds @11:50:31.94 Pressure.stop @11:50:32.03 Intake.close @11:50:32.79 Pressurized cartridge with 0.699ml to 26.2psi @11:50:32.81 Isolated @34.7psia (20.6psi) @11:50:32.84 Verifying cartridge holds at least 0.65..0.85 of 34.7psia @11:50:32.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:50:37.51 Cartridge pressure fell 1% to 34.5psia @11:50:37.65 Sampling 50.0ml @11:50:37.67 SamplePump.setPosition! 0ml @11:50:37.75 Exhaust.open @11:50:38.46 Intake.open @11:50:39.32 Pressure.in :default @11:50:39.43 Pressure.hold @11:50:39.60 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:50:39.82 SamplePump.in :sample @11:50:39.92 SamplePump.seek 50ml,4:41 @11:50:42.94 Cartridge 16 filtering 1/50ml at 0.48ml/s with 26.9psi vacuum @11:51:42.94 Cartridge 16 filtering 28/50ml at 0.38ml/s with 18.3psi vacuum @11:52:39.13 Pressure.stop @11:52:39.31 Intake.close @11:52:39.39 Exhaust.close @11:52:39.64 Toroid.in :hold @11:52:39.74 Toroid.seek :bypass @11:52:41.40 Toroid.in :default @11:52:41.51 Toroid.seek :clear @11:52:43.15 Sampled 50.0ml @11:52:43.69 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 16" @11:52:45.85 SPR.command "read_sequences" @11:52:46.29 SPR.command "stats_on" @11:52:46.36 Gate.power :sampler,:OFF @11:52:46.54 SPR.command "start 0 1" @11:52:47.25 SPR.start /var/log/esp/spr23aug1411h.spr.bz2 @11:52:49.15 Handoff.select :waste @11:52:49.71 upsync @11:52:55.92 Syringe tare is 18% of full scale @11:52:55.96 PinPort.in :locking @11:52:56.07 PinPort.seek :locked @11:52:58.65 Archive.in :fast @11:52:58.71 Lysis2.in :fast @11:52:58.79 Lysis1.seek 1350uL @11:52:58.97 Archive.seek :full @11:52:59.14 Lysis2.seek 950uL @11:53:03.91 Plunge.seek :reagent @11:53:04.89 Evacuating puck with filter bubble point of 50psi @11:53:04.92 PinPort.seek :locked @11:53:05.09 Handoff.select :waste /home/esp/esp2/lib/thread.rb:808: [BUG] gc_sweep(): unknown data type 0x0(0xb694a2c0) ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] @12:15:21.45PDT14-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @12:15:25.05 >BREAK< ESP3 Gateway boot v1.43 for core bus @12:15:25.08 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @12:15:28.69 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @12:15:29.33 Sea pressure (Abs) tare is 6% of full scale @12:15:40.63 Polling Can every 2:00 @12:15:40.70 Waiting for espclients... @12:15:41.21 Session Begun @12:16:41.35 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 16 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1411h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @12:17:38.15 -> showlog -20 @12:17:48.95 -> showlog -100 @12:18:34.71 -> showlog -200 @12:24:37.26 Session Begun @12:24:41.91 -> showlog -100 @12:28:16.62 -> showlog -300 @12:34:07.56 -> LyS1 @12:34:07.64 Gate.power :sampler,:OFF,limit to 3500mA @12:34:07.66 Gate.power :main,:ON,limit to 5000mA @12:34:07.73 Gate.power :analytic1,:ON,limit to 5000mA @12:34:07.76 Gate.power :analytic2,:ON,limit to 5000mA @12:34:07.97 Powering core bus @12:34:10.22 SPR Module 1, FW Rev. B, FPGA Rev. C @12:34:10.75 Archive.configure :default @12:34:10.89 Air.configure :default @12:34:11.00 Lysis1.configure :default @12:34:11.14 Lysis2.configure :default @12:34:11.28 Plunge.configure :default @12:34:11.42 SamplePump.configure :default @12:34:11.56 Toroid.configure :default @12:34:11.71 Carousel.configure :default @12:34:11.84 Pressure.configure :default @12:34:11.98 ExtraPump.configure :default @12:34:12.09 PinPort.configure :default @12:34:12.20 All elf controllers running firmware version 4.84 @12:34:12.27 Gate.power :motor,:ON,limit to 5000mA @12:34:12.43 Slide::Error in bill -- Lysis Syringe #1 (LyS1) Not yet Homed! @12:34:44.03 -> SPR.start @12:34:45.21 SPR.command "read_sequences" @12:34:46.00 SPR.command "stats_on" # @12:34:46.90 SPR.start /var/log/esp/spr23aug1412h.spr.bz2 @12:34:56.52 -> SPR.command "start 2 14" @12:34:56.54 SPR.command "start 2 14" @12:35:04.18 -> SPR.stop @12:35:04.36 Ended log spr23aug1412h.spr.bz2 @12:35:04.38 SPR.command "stats_off" @12:36:13.18 -> exit @12:36:13.21 Session Ended @12:37:23.14 Session Begun @12:38:25.55 -> #Hey Bill, I just saw your message on slack, let me know if there is anything I can help with @12:38:56.84 -> #I'm waiting to see what Chris is typing... @12:42:56.67 -> exit @12:42:56.69 Session Ended @12:51:36.38 Session Begun @12:51:44.21 -> Cartridge.dry [6,7,8,9,10,11,12,13,14,15] @12:52:00.77 -> Cartridge.dry [6,7,8,9,10,11,12,13,14,15] @12:52:30.61 -> cd esp2local @12:52:30.67 NoMethodError in bill -- undefined method `cd' for CmdServer::Client:Class @12:52:33.33 -> exit @12:52:33.36 Session Ended @12:53:49.73 -> exit @12:53:49.75 Session Ended @12:57:59.20 Session Begun @13:04:38.16 Session Begun @13:04:48.08 -> ESP.configure @13:04:48.29 Gate.power :sampler,:OFF,limit to 3500mA @13:04:48.31 Gate.power :analytic1,:ON,limit to 5000mA @13:04:48.39 Gate.power :analytic2,:ON,limit to 5000mA @13:04:48.71 Archive.configure :default @13:04:48.91 Air.configure :default @13:04:49.08 Lysis1.configure :default @13:04:49.31 Lysis2.configure :default @13:04:49.52 Plunge.configure :default @13:04:49.72 SamplePump.configure :default @13:04:49.93 Toroid.configure :default @13:04:50.13 Carousel.configure :default @13:04:50.33 Pressure.configure :default @13:04:50.53 ExtraPump.configure :default @13:04:50.71 PinPort.configure :default @13:04:50.85 All elf controllers running firmware version 4.84 @13:06:09.75 -> ESP Archive = Archive Syringe (ArS) Not yet Homed! Air = Air Syringe (AirS) Not yet Homed! Lysis1 = Lysis Syringe #1 (LyS1) Not yet Homed! Lysis2 = Lysis Syringe #2 (LyS2) Not yet Homed! Plunge = Plunge Valve (PV) Not yet Homed! Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) Not yet Homed! Carousel = Storage Carousel (SC) Not yet Homed! Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) Not yet Homed! @13:06:11.82 Session Begun @13:06:25.11 -> slots @13:06:25.17 -> Archive.home.jog 0 @13:06:25.30 Archive.home.jog 0 {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 16 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1411h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} Archive Syringe (ArS) at 1724uL @13:06:37.46 -> Lysis1.home.jog 0 @13:06:37.50 Lysis1.home.jog 0 Lysis Syringe #1 (LyS1) at 1350uL @13:06:46.90 -> Lysis2.home.jog 0 @13:06:46.94 Lysis2.home.jog 0 Lysis Syringe #2 (LyS2) at 950uL @13:07:09.94 -> #Ben, I'm trying to manually recover. Please stand by... @13:07:30.34 -> Pinport.home.jog 0 @13:07:30.42 NameError in brent -- uninitialized constant Pinport @13:07:36.11 -> PP.home.jog 0 @13:07:36.18 PinPort.home.jog 0 Handoff Pin Port (PP) at cartridge - 3 counts @13:07:48.35 -> ESP Archive = Archive Syringe (ArS) at 1724uL Air = Air Syringe (AirS) Not yet Homed! Lysis1 = Lysis Syringe #1 (LyS1) at 1350uL Lysis2 = Lysis Syringe #2 (LyS2) at 950uL Plunge = Plunge Valve (PV) Not yet Homed! Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) Not yet Homed! Carousel = Storage Carousel (SC) Not yet Homed! Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) at cartridge - 3 counts @13:07:55.72 -> Air.home.jog 0 @13:07:55.78 Air.home.jog 0 Air Syringe (AirS) at 1562uL @13:08:04.84 -> Plunge.home.jog 0 @13:08:04.92 Plunge.home.jog 0 Plunge Valve (PV) at reagentLG + 16 counts @13:08:12.66 -> Handoff.waste @13:08:12.69 Handoff.select :waste Handoff Valve::Manifold selects waste @13:09:07.38 -> Toroid.bypass @13:09:07.55 Slide::Error in brent -- Storage Carousel (SC) Not yet Homed! @13:09:14.74 -> ESP Archive = Archive Syringe (ArS) at 1724uL Air = Air Syringe (AirS) at 1562uL Lysis1 = Lysis Syringe #1 (LyS1) at 1350uL Lysis2 = Lysis Syringe #2 (LyS2) at 950uL Plunge = Plunge Valve (PV) at reagentLG + 16 counts Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) Not yet Homed! Carousel = Storage Carousel (SC) Not yet Homed! Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) at cartridge - 3 counts @13:09:54.42 -> Toroid.home.jog 0 @13:09:54.46 Toroid.home.jog 0 Toroid Valve (TV) at clear + 21 counts @13:09:59.07 -> Toroid.clear @13:09:59.19 Toroid.seek :clear Toroid Valve (TV) clear @13:10:16.42 -> Carousel.home.jog 0 @13:10:16.46 Carousel.home.jog 0 Storage Carousel (SC) at 16 - 2 counts @13:10:35.56 -> Carousel.align @13:10:35.64 NoMethodError in brent -- undefined method `align' for Carousel:Slide @13:11:31.39 -> SC.to 16 @13:11:31.43 Carousel.seek 16 Storage Carousel (SC) at 16 @13:11:39.07 -> ESP Archive = Archive Syringe (ArS) at 1724uL Air = Air Syringe (AirS) at 1562uL Lysis1 = Lysis Syringe #1 (LyS1) at 1350uL Lysis2 = Lysis Syringe #2 (LyS2) at 950uL Plunge = Plunge Valve (PV) at reagentLG + 16 counts Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 16 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) at cartridge - 3 counts @13:12:04.83 -> PP.to :cartridge @13:12:04.89 PinPort.seek :cartridge Handoff Pin Port (PP) cartridge @13:12:59.56 -> Lysis2.legend {-11650 => "extended", -8415 => ["empty",0,:end], -1020 => ["locked",1730,:lock,:cartridge,:full], 0 => ["clear",:home,:free,:begin], 50 => :limit} @13:13:40.75 -> Cartridge.mount @13:13:41.23 Air.in :fast @13:13:41.36 Air.seek :locked @13:13:41.58 Carousel.reconfigure :locked @13:13:41.80 Powering cartridge bus @13:13:44.46 Gate.setIR 1750,5000,0,6 @13:13:44.60 Gate.setIR 0,5000,0,6 @13:13:44.73 PuckHeater.configure :default @13:13:44.86 Gate.power :heat,:ON,limit to 5000mA @13:13:45.61 Mounted lysis Cartridge board#Q05080 @13:13:45.71 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @13:14:02.88 -> Cartridge.type :microSPR_phyto @13:14:15.39 -> Syringe.tare @13:14:22.00 Syringe tare is 18% of full scale 0.001529827(PSI/count)-17.8 @13:15:06.66 -> Archive Archive Syringe (ArS) at 1724uL @13:19:15.66 -> Cartridge.processingOptions {} @13:19:45.85 -> microSPR @13:19:45.92 SPR.command "stats_on" @13:19:46.48 SPR.command "start 0 1" @13:19:47.18 SPR.start /var/log/esp/spr23aug1413h.spr.bz2 @13:19:49.21 Handoff.select :waste @13:19:55.04 Session Begun @13:19:55.84 Syringe tare is 18% of full scale @13:19:55.95 PinPort.in :locking @13:19:56.08 PinPort.seek :locked @13:19:56.28 Archive.in :fast @13:19:56.36 Lysis2.in :fast @13:19:56.52 Lysis1.in :fast @13:19:56.68 Archive.seek :full @13:19:56.99 Lysis2.seek 950uL @13:19:57.27 Lysis1.seek 1350uL @13:19:57.59 Plunge.seek :reagent @13:19:57.96 Evacuating puck with filter bubble point of 50psi @13:19:58.03 PinPort.seek :locked @13:19:58.25 Handoff.select :waste @13:20:04.96 Air syringe pumping up to 1 strokes to reach 8psi @13:20:04.97 Air stroke #1 of 1 @13:20:05.27 Air.in :pressurize @13:20:05.41 Air.seek :pressurized @13:20:11.26 0.3psi after pressurize @13:20:11.37 Air.in :compress @13:20:11.60 Air.seek :evac @13:20:14.72 0.8psi after compress @13:20:25.33 0.4psi after compress (stable) @13:20:25.41 Air.in :default @13:20:25.56 Air.hold @13:20:25.95 at 0.2psi Opened to waste @13:20:25.97 Handoff.select :blocked @13:20:26.22 Archive syringe pumping up to 8 strokes to reach 50psi @13:20:26.24 Archive stroke #1 of 8 @13:20:26.47 Archive.in :pressurize @13:20:26.69 Archive.seek :pressurized @13:20:39.29 4.1psi after pressurize @13:21:05.44 13.1psi after pressurize (stable) @13:21:05.51 Archive.in :compress @13:21:05.70 Archive.seek :empty @13:21:20.43 13.5psi after compress @13:21:31.05 19.2psi after compress (stable) @13:21:31.16 Archive.in :gulp @13:21:31.33 Archive.seek :topVent @13:21:37.68 19.2psi after gulp @13:21:43.38 19.2psi after gulp (stable) @13:21:43.42 Archive stroke #2 of 8 @13:21:43.72 Archive.in :pressurize @13:21:43.88 Archive.seek :pressurized @13:21:56.54 19.3psi after pressurize @13:22:15.64 24.0psi after pressurize (stable) @13:22:15.73 Archive.in :compress @13:22:15.88 Archive.seek :empty @13:22:30.61 33.3psi after compress @13:22:36.55 Session Begun @13:22:39.86 -> hidelog Log display suppressed @13:22:41.22 34.9psi after compress (stable) @13:22:41.32 Archive.in :gulp @13:22:41.46 Archive.seek :topVent @13:22:47.81 35.1psi after gulp @13:22:53.44 35.1psi after gulp (stable) @13:22:53.47 Archive stroke #3 of 8 @13:22:53.69 Archive.in :pressurize @13:22:53.85 Archive.seek :pressurized @13:23:06.44 35.1psi after pressurize @13:23:12.06 35.1psi after pressurize (stable) @13:23:12.19 Archive.in :compress @13:23:12.37 Archive.seek :empty @13:23:27.08 48.4psi after compress @13:23:37.74 49.7psi after compress (stable) @13:23:37.84 Archive.in :gulp @13:23:37.99 Archive.seek :topVent @13:23:44.40 49.8psi after gulp @13:23:50.05 49.7psi after gulp (stable) @13:23:50.06 Archive stroke #4 of 8 @13:23:50.31 Archive.in :pressurize @13:23:50.46 Archive.seek :pressurized @13:24:03.06 49.9psi after pressurize @13:24:08.66 49.9psi after pressurize (stable) @13:24:08.70 Archive.in :compress @13:24:08.95 Archive.seek :empty @13:24:09.29 Reached 50.1 of 50.0psi after pumping 6608uL (3.82 strokes) @13:24:09.32 Archive.in :default @13:24:09.52 Archive.hold @13:24:09.70 Handoff.select :waste @13:24:33.01 delay 1:00 @13:25:33.05 Handoff.select :blocked @13:25:55.90 Handoff.select :waste @13:25:56.11 0.6psi at evacEnd @13:25:56.14 Handoff.select :blocked @13:25:56.42 Archive.in :fast @13:25:56.56 Archive.seek :topVent @13:26:09.31 Stable @0.8psi after Handoff blocked @13:26:09.44 Archive syringe pumping up to 8 strokes to reach 31psi @13:26:09.48 Archive stroke #1 of 8 @13:26:09.80 Archive.in :pressurize @13:26:10.00 Archive.seek :pressurized @13:26:22.60 1.3psi after pressurize @13:26:22.62 Archive.in :compress @13:26:22.83 Archive.seek :empty @13:26:37.55 12.3psi after compress @13:26:48.17 12.9psi after compress (stable) @13:26:48.25 Archive.in :gulp @13:26:48.41 Archive.seek :topVent @13:26:54.77 13.0psi after gulp @13:27:00.38 13.0psi after gulp (stable) @13:27:00.39 Archive stroke #2 of 8 @13:27:00.64 Archive.in :pressurize @13:27:00.80 Archive.seek :pressurized @13:27:13.41 13.0psi after pressurize @13:27:13.51 Archive.in :compress @13:27:13.65 Archive.seek :empty @13:27:28.39 24.1psi after compress @13:27:39.02 24.7psi after compress (stable) @13:27:39.09 Archive.in :gulp @13:27:39.29 Archive.seek :topVent @13:27:45.64 24.8psi after gulp @13:27:51.25 24.7psi after gulp (stable) @13:27:51.26 Archive stroke #3 of 8 @13:27:51.49 Archive.in :pressurize @13:27:51.66 Archive.seek :pressurized @13:28:04.24 24.7psi after pressurize @13:28:04.33 Archive.in :compress @13:28:04.55 Archive.seek :empty @13:28:14.12 Reached 30.9 of 30.8psi after pumping 5078uL (2.94 strokes) @13:28:14.15 Archive.in :default @13:28:14.47 Archive.hold @13:28:14.64 Handoff.select :waste @13:28:35.47 delay 1:00 @13:29:35.49 Handoff.select :blocked @13:29:58.30 Handoff.select :waste @13:29:58.50 0.9psi at evacEnd @13:29:58.54 Air.seek :fullVent @13:30:00.12 Archive.in :fast @13:30:00.27 Archive.seek :topVent @13:30:07.04 Seawater evacuated, Pressure above/below filter released. @13:30:07.26 Lysis1.in :slow @13:30:07.47 Lysis1.seek 1050uL @13:30:37.55 delay 5 seconds @13:30:42.67 Handoff.select :blocked @13:30:42.96 Lysis1.seek 450uL @13:31:42.98 delay 3 seconds @13:31:46.09 Air.in mix with 1.40048 parts Lysis1 @13:31:46.89 Air.seek :evac @13:31:47.07 Lysis1.seek 300uL @13:32:02.29 Lysis1.seek :empty @13:32:32.49 Lysis buffer delivered @6.5psi @13:32:32.50 Polling Can every 2:00 @13:32:33.16 PuckHeater.configure :default #ambient 25.2C @13:32:33.31 PuckHeater.seek 79.6C @13:33:24.66 PuckHeater.hold 2:00,79.6C @13:35:24.54 Polling Can every 2:00 @13:35:25.18 PuckHeater.configure :default #ambient 25.2C @13:35:25.34 PuckHeater.hold 30 seconds,78.6C @13:35:55.54 Polling Can every 2:00 @13:35:56.10 PuckHeater.hold 30 seconds,78.1C @13:36:05.15 -> list method :startDoublet def startDoublet start {fire :archiveHiBiomass_bac, :microSPR_phyto} end def doubleRegen #2x Regen "hail-Mary" intended to remove residual oil from SPR ESP.ready! SPR.start delay 2 SPR.command "start 3 0" delay 15.minutes SPR.command "start 2 14" ensure SPR.stop delay 10 Cmd.stop end class Sampler unless @@default.key? :recoveryVolume @13:36:26.32 Polling Can every 2:00 @13:36:26.88 PuckHeater.hold 10:00,77.5C @13:46:25.39 Lysis complete. Syringe pressure @8.7psi @13:46:25.41 Archive syringe pumping up to 5 strokes to reach 40psi @13:46:25.48 Archive stroke #1 of 5 @13:46:25.72 Archive.in :pressurize @13:46:25.87 Archive.seek :pressurized @13:46:38.46 8.7psi after pressurize @13:46:44.07 8.6psi after pressurize (stable) @13:46:44.14 Archive.in :compress @13:46:44.34 Archive.seek :empty @13:46:59.06 27.3psi after compress @13:47:11.19 30.8psi after compress (stable) @13:47:11.27 Archive.in :gulp @13:47:11.43 Archive.seek :topVent @13:47:17.78 30.8psi after gulp @13:47:23.38 30.6psi after gulp (stable) @13:47:23.39 Archive stroke #2 of 5 @13:47:23.63 Archive.in :pressurize @13:47:23.78 Archive.seek :pressurized @13:47:36.40 30.4psi after pressurize @13:47:42.03 30.3psi after pressurize (stable) @13:47:42.13 Archive.in :compress @13:47:42.25 Archive.seek :empty @13:47:52.49 Reached 40.2 of 40.0psi after pumping 3362uL (1.94 strokes) @13:47:52.53 Archive.in :default @13:47:52.72 Archive.hold @13:47:52.89 delay 2 seconds @13:47:54.92 Archive.in :fast @13:47:55.07 Archive.seek :full @13:48:02.08 Lysis1.seek 138uL @13:48:16.04 delay 1 second @13:48:17.08 Archive.seek :full @13:48:17.25 Lysis1.seek :empty @13:48:31.28 Handoff.select :blocked @13:48:31.47 Initial air pressure @44.2psi @13:48:31.64 Lysis1.in :extract @13:48:31.83 Lysis1.seek 600uL,nil @13:48:37.55 Initial 600uL lysate @21.6psi @13:48:37.57 Handoff.select :blocked @13:48:37.84 Initial air pressure @21.8psi @13:48:38.01 Lysis1.seek 825uL,nil @13:48:40.52 Recovered 825uL lysate @18.3psi @13:48:40.56 Air.in :halfSpeed @13:48:40.79 Air.seek :fullVent @13:48:43.57 Puck Top Vented, bottom @8.7psi @13:48:43.60 Handoff.select :waste @13:48:43.86 delay 5 seconds @13:48:48.95 Handoff open to waste, bottom @0.7psi @13:48:49.03 Air.in :default @13:48:49.28 Air.seek :evac @13:48:50.79 Lysis2.in :DAmix @13:48:51.06 Lysis1.in :DAmix @13:48:51.77 Lysis2.seek 700uL @13:48:51.93 Lysis1.seek 575uL @13:49:27.09 delay 5 seconds @13:49:32.12 Handoff.select SPR @13:49:32.32 SPR.command "start 2 14" @13:49:33.07 SPR stabilized @13:49:33.36 delay 10 seconds @13:49:43.38 SPR.command "start 2 0" @13:49:46.43 Waiting up to 11:00 for SPR to enter state 1 @13:51:44.10 SPR.command "hold_on" @13:51:46.01 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @13:51:46.74 Lysis2.seek 400uL @13:51:46.95 Lysis1.seek 275uL @13:52:29.04 delay 15 seconds @13:52:44.06 SPR.command "hold_off" @13:52:47.09 Waiting up to 38:00 for SPR to enter state 7 @14:02:30.91 -> exit @14:02:30.94 Session Ended @14:07:06.32 SPRsummary:-428RIU,2.67e+09RIU,17.4RIU,39.7RIU after 3018s @14:18:36.76 SPR analysis completed @14:18:44.58 Ended log spr23aug1413h.spr.bz2 @14:18:44.60 SPR.command "stats_off" @14:23:23.31 Session Begun @14:23:25.79 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: spr23aug1413h.spr.bz2}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 52..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @14:24:17.45 -> Cartridge.release 50 1 @14:28:33.47 -> exit @14:28:33.50 Session Ended @14:28:44.27 -> Cmd.stop @14:28:44.45 Handoff.select :close @14:28:44.67 Gate.power :heat,:OFF @14:28:44.69 Gate.power :cartridge,:OFF @14:28:44.88 PinPort.in :unlocking @14:28:45.00 PinPort.seek :clear @14:28:47.01 Carousel.in :default @14:28:47.24 Carousel.home.seek 16 @14:28:47.64 Plunge.seek :locked @14:28:48.60 Archive.in :unlocking @14:28:48.84 Archive.seek :clear @14:28:48.92 Air.in :unlocking @14:28:49.25 Air.seek :clear @14:28:49.44 Lysis1.in :unlocking @14:28:49.66 Lysis1.seek :clear @14:28:49.85 Lysis2.in :unlocking @14:28:49.98 Lysis2.seek :clear @14:28:53.89 Gate.power :motor,:OFF @14:28:53.92 Gate.power :core,:OFF @14:28:53.99 Gate.power :analytic1,:OFF @14:28:54.04 Gate.power :analytic2,:OFF @14:28:54.11 Gate.power :main,:OFF @14:28:54.16 Safely stopped and ready to power off :STOPPED @14:29:02.59 -> MainThread.exit # @14:29:02.77 ThreadError in bill -- killed thread @14:29:02.85 Session Ended @14:29:02.94 Session Ended @14:29:03.02 Session Ended @14:29:03.11 Session Ended @14:31:08.19PDT14-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @14:31:10.30 >BREAK< ESP3 Gateway boot v1.43 for core bus @14:31:10.32 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @14:31:13.77 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @14:31:14.38 Sea pressure (Abs) tare is 6% of full scale @14:31:25.57 Polling Can every 2:00 @14:31:25.62 Loading /home/esp/esp2/bin/processAll mission @14:31:25.84 Sea pressure (Abs) tare is 6% of full scale @14:31:25.86 Sampling default goal volumes @14:31:25.88 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @14:31:25.94 Gate.power :sampler,:OFF,limit to 3500mA @14:31:25.95 Gate.power :main,:ON,limit to 5000mA @14:31:25.97 Gate.power :analytic1,:ON,limit to 5000mA @14:31:25.98 Gate.power :analytic2,:ON,limit to 5000mA @14:31:26.14 Powering core bus @14:31:26.38 Session Begun @14:31:28.41 SPR Module 1, FW Rev. B, FPGA Rev. C @14:31:28.98
Archive.configure :default @14:31:29.12 Air.configure :default @14:31:29.22 Lysis1.configure :default @14:31:29.31 Lysis2.configure :default @14:31:29.42 Plunge.configure :default @14:31:29.54 SamplePump.configure :default @14:31:29.66 Toroid.configure :default @14:31:29.78 Carousel.configure :default @14:31:29.89 Pressure.configure :default @14:31:30.01 ExtraPump.configure :default @14:31:30.10 PinPort.configure :default @14:31:30.18 All elf controllers running firmware version 4.84 @14:31:30.21 Gate.power :motor,:ON,limit to 5000mA @14:31:30.42 Archive.home.jog 0 @14:31:31.22 Carousel.home.jog 0 @14:31:31.44 Plunge.home.jog 0 @14:31:31.50 Lysis2.home.jog 0 @14:31:31.55 Lysis1.home.jog 0 @14:31:31.59 Air.home.jog 0 @14:31:31.69 Toroid.home.jog 0 @14:31:31.72 PinPort.home.jog 0 @14:31:32.41 Archive.in :homing @14:31:32.81 Archive.jog 1373 @14:31:32.88 PinPort.in :homing @14:31:32.93 Plunge.in :homing @14:31:32.99 Lysis1.in :homing @14:31:33.03 Toroid.in :homing @14:31:33.11 Air.in :homing @14:31:33.19 Lysis2.in :homing @14:31:33.53 PinPort.jog -107 @14:31:33.58 Plunge.jog 1593 @14:31:33.67 Toroid.jog 616 @14:31:33.71 Lysis1.jog 1383 @14:31:33.79 Air.jog 1382 @14:31:33.84 Lysis2.jog 1396 @14:31:34.67 Archive.setRawPosition! 50 @14:31:34.70 PinPort.setRawPosition! 0 @14:31:34.81 Archive.seek :home @14:31:34.86 PinPort.seek :home @14:31:35.05 PinPort.in :default @14:31:35.25 Plunge.setRawPosition! 50 @14:31:35.28 Lysis1.setRawPosition! 50 @14:31:35.34 Archive.in :default @14:31:35.44 Plunge.seek :home @14:31:35.53 Lysis1.seek :home @14:31:35.79 Air.setRawPosition! 50 @14:31:35.82 Lysis2.setRawPosition! 50 @14:31:35.97 Lysis2.seek :home @14:31:36.00 Air.seek :home @14:31:36.25 Toroid.setRawPosition! 0 @14:31:36.29 Plunge.in :default @14:31:36.37 Lysis1.in :default @14:31:36.47 Toroid.seek :home @14:31:36.70 Toroid.in :default @14:31:36.75 Lysis2.in :default @14:31:36.80 Air.in :default @14:31:37.27
Toroid.seek :clear @14:31:38.89 Carousel.in :default @14:31:39.05 Carousel.home.seek 16 @14:31:39.37 Selecting Cartridge 50 @14:31:39.71 Carousel.seek 50 @14:31:47.00 Lysis1.in :fast @14:31:47.12 Lysis1.seek :locked @14:31:49.78 Plunge.seek :locked @14:31:49.98 Air.in :fast @14:31:50.07 Air.seek :locked @14:31:51.67
Carousel.reconfigure :locked @14:31:51.87 Powering cartridge bus @14:31:54.53 Gate.setIR 1750,5000,0,6 @14:31:54.66 Gate.setIR 0,5000,0,6 @14:31:54.76 PuckHeater.configure :default @14:31:54.85 Gate.power :heat,:ON,limit to 5000mA @14:31:55.53 Mounted lysis Cartridge @14:31:55.56 WARNING: Using default puck temperature sensor calibration @14:31:55.59 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:31:57.21 SPR.command "read_sequences" @14:31:57.61 SPR.command "stats_on" @14:31:57.78 SPR.command "start 0 1" @14:31:58.49 SPR.start /var/log/esp/spr23aug1414h.spr.bz2 @14:32:00.46 Waiting for baseline SPR stabilization @14:32:00.48 Handoff.select :waste @14:32:07.23 Syringe tare is 18% of full scale @14:32:07.25 PinPort.in :locking @14:32:07.37 PinPort.seek :locked @14:32:09.93 Archive.in :fast @14:32:09.98 Lysis2.in :fast @14:32:10.10 Lysis1.seek 1000uL @14:32:10.21 Archive.seek :full @14:32:10.38 Lysis2.seek 1000uL @14:32:15.00 delayUntil "14:42:00.01" @14:42:00.02 Lysis2.in :DAmix @14:42:00.18 Lysis1.in :DAmix @14:42:00.74 Lysis2.seek 750uL @14:42:00.77 Lysis1.seek 750uL @14:42:35.90 delay 5 seconds @14:42:40.93 Handoff.select SPR @14:42:41.14 SPR.command "start 2 14" @14:42:41.74 SPR stabilized @14:42:41.92 delay 10 seconds @14:42:51.94 SPR.command "start 2 0" @14:42:55.13 Waiting up to 11:00 for SPR to enter state 1 @14:44:52.77 SPR.command "hold_on" @14:44:54.16 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:44:54.80 Lysis2.seek 450uL @14:44:54.88 Lysis1.seek 450uL @14:45:36.94 delay 15 seconds @14:45:51.97 SPR.command "hold_off" @14:45:54.82 Waiting up to 38:00 for SPR to enter state 7 @15:00:14.99 SPRsummary:10.9RIU,5.96RIU,71.9RIU,92.4RIU after 1791s @15:11:45.57 delay 10 seconds @15:11:55.59 SPR.command "start 2 0" @15:11:58.28 Waiting up to 11:00 for SPR to enter state 1 @15:13:55.93 SPR.command "hold_on" @15:13:57.33 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @15:13:57.91 Lysis2.seek 300uL @15:13:57.98 Lysis1.seek 300uL @15:14:19.11 delay 15 seconds @15:14:34.15 SPR.command "hold_off" @15:14:37.17 Waiting up to 38:00 for SPR to enter state 7 @15:40:27.93 delay 10 seconds @15:40:37.95 SPR.command "start 2 0" @15:40:40.63 Waiting up to 11:00 for SPR to enter state 1 @15:42:38.29 SPR.command "hold_on" @15:42:40.22 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @15:42:40.88 Lysis2.seek 150uL @15:42:40.99 Lysis1.seek 150uL @15:43:02.06 delay 15 seconds @15:43:17.08 SPR.command "hold_off" @15:43:20.48 Waiting up to 38:00 for SPR to enter state 7 @16:09:10.29 delay 10 seconds @16:09:20.32 SPR.command "start 2 0" @16:09:22.99 Waiting up to 11:00 for SPR to enter state 1 @16:11:20.67 SPR.command "hold_on" @16:11:22.05 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:11:22.75 Lysis2.seek 0uL @16:11:22.82 Lysis1.seek 0uL @16:11:43.95 delay 15 seconds @16:11:58.97 SPR.command "hold_off" @16:12:02.42 Waiting up to 38:00 for SPR to enter state 7 @16:37:53.46 SPR analysis completed @16:37:57.52 Ended log spr23aug1414h.spr.bz2 @16:37:57.54 SPR.command "stats_off" @16:37:57.85 Handoff.select :close @16:37:58.04
Gate.power :heat,:OFF @16:37:58.06 Gate.power :cartridge,:OFF @16:37:58.18 PinPort.in :unlocking @16:37:58.27 PinPort.seek :clear @16:38:00.22 Carousel.in :default @16:38:00.37 Carousel.home.seek 50 @16:38:00.73 Plunge.seek :locked @16:38:00.94 Archive.in :unlocking @16:38:01.15 Archive.seek :clear @16:38:01.21 Air.in :unlocking @16:38:01.47 Air.seek :clear @16:38:01.61 Lysis1.in :unlocking @16:38:02.29 Lysis1.seek :clear @16:38:02.38 Lysis2.in :unlocking @16:38:02.51 Lysis2.seek :clear @16:38:07.15
Gate.power :motor,:OFF @16:38:07.17 Gate.power :core,:OFF @16:38:07.21 Gate.power :analytic1,:OFF @16:38:07.24 Gate.power :analytic2,:OFF @16:38:07.28 Gate.power :main,:OFF @16:38:07.30 Safely stopped and ready to power off @16:38:07.84 Cartridge::None in MAIN -- Not enough standard cartridges remain @16:38:07.93 Session Ended @16:40:12.56PDT14-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @16:40:14.21 >BREAK< ESP3 Gateway boot v1.43 for core bus @16:40:14.24 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @16:40:18.10 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @16:40:18.69 Sea pressure (Abs) tare is 6% of full scale @16:40:29.79 Polling Can every 2:00 @16:40:29.83 Waiting for espclients... @16:40:30.57 Session Begun @16:40:35.11 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, [52,51] => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @16:41:28.94 -> Cartridge.release 52 1 @16:41:40.70 -> MainThread.exit # @16:41:40.80 ThreadError in bill -- killed thread @16:41:40.83 Session Ended @16:42:03.22PDT14-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @16:42:05.41 >BREAK< ESP3 Gateway boot v1.43 for core bus @16:42:05.43 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @16:42:08.81 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @16:42:09.45 Sea pressure (Abs) tare is 6% of full scale @16:42:20.55 Polling Can every 2:00 @16:42:20.59 Loading /home/esp/esp2/bin/processAll mission @16:42:20.81 Sea pressure (Abs) tare is 6% of full scale @16:42:20.82 Sampling default goal volumes @16:42:20.84 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @16:42:20.94 Gate.power :sampler,:OFF,limit to 3500mA @16:42:21.05 Gate.power :main,:ON,limit to 5000mA @16:42:21.13 Gate.power :analytic1,:ON,limit to 5000mA @16:42:21.20 Session Begun @16:42:21.28
Gate.power :analytic2,:ON,limit to 5000mA @16:42:21.59 Powering core bus @16:42:23.74 SPR Module 1, FW Rev. B, FPGA Rev. C @16:42:24.36
Archive.configure :default @16:42:24.48 Air.configure :default @16:42:24.57 Lysis1.configure :default @16:42:24.66 Lysis2.configure :default @16:42:24.78 Plunge.configure :default @16:42:24.89 SamplePump.configure :default @16:42:25.01 Toroid.configure :default @16:42:25.15 Carousel.configure :default @16:42:25.27 Pressure.configure :default @16:42:25.39 ExtraPump.configure :default @16:42:25.47 PinPort.configure :default @16:42:25.57 All elf controllers running firmware version 4.84 @16:42:25.60 Gate.power :motor,:ON,limit to 5000mA @16:42:25.95 Archive.home.jog 0 @16:42:26.56 Carousel.home.jog 0 @16:42:26.71 Toroid.home.jog 0 @16:42:26.78 Lysis2.home.jog 0 @16:42:26.82 Air.home.jog 0 @16:42:26.85 Lysis1.home.jog 0 @16:42:26.93 PinPort.home.jog 0 @16:42:26.98 Plunge.home.jog 0 @16:42:27.77 Archive.in :homing @16:42:27.96 Archive.jog 1387 @16:42:28.20 PinPort.in :homing @16:42:28.27 Toroid.in :homing @16:42:28.31 Lysis1.in :homing @16:42:28.37 Plunge.in :homing @16:42:28.43 Air.in :homing @16:42:28.48 Lysis2.in :homing @16:42:28.74 PinPort.jog -107 @16:42:28.80 Toroid.jog 591 @16:42:28.99 Lysis2.jog 1393 @16:42:29.05 Air.jog 1364 @16:42:29.13 Plunge.jog 1570 @16:42:29.19 Lysis1.jog 1385 @16:42:29.95 PinPort.setRawPosition! 0 @16:42:29.97 Archive.setRawPosition! 50 @16:42:30.11 PinPort.seek :home @16:42:30.15 Archive.seek :home @16:42:30.36 PinPort.in :default @16:42:30.49 Lysis2.setRawPosition! 50 @16:42:30.59 Lysis2.seek :home @16:42:30.71 Archive.in :default @16:42:30.90 Lysis2.in :default @16:42:31.09 Toroid.setRawPosition! 0 @16:42:31.13 Plunge.setRawPosition! 50 @16:42:31.17 Air.setRawPosition! 50 @16:42:31.22 Lysis1.setRawPosition! 50 @16:42:31.40 Toroid.seek :home @16:42:31.47 Plunge.seek :home @16:42:31.53 Air.seek :home @16:42:31.62 Lysis1.seek :home @16:42:31.92 Toroid.in :default @16:42:32.09 Lysis1.in :default @16:42:32.12 Air.in :default @16:42:32.17 Plunge.in :default @16:42:32.66
Toroid.seek :clear @16:42:34.30 Carousel.in :default @16:42:34.46 Carousel.home.seek 50 @16:42:34.77 Selecting Cartridge 52 @16:42:35.12 Carousel.seek 52 @16:42:35.91 Lysis1.in :fast @16:42:36.01 Lysis1.seek :locked @16:42:38.65 Plunge.seek :locked @16:42:38.84 Air.in :fast @16:42:38.93 Air.seek :locked @16:42:40.52
Carousel.reconfigure :locked @16:42:40.73 Powering cartridge bus @16:42:43.40 Gate.setIR 1750,5000,0,6 @16:42:43.52 Gate.setIR 0,5000,0,6 @16:42:43.62 PuckHeater.configure :default @16:42:43.71 Gate.power :heat,:ON,limit to 5000mA @16:42:44.39 Mounted lysis Cartridge @16:42:44.42 WARNING: Using default puck temperature sensor calibration @16:42:44.45 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:42:46.07 SPR.command "read_sequences" @16:42:46.23 SPR.command "stats_on" @16:42:46.40 SPR.command "start 0 1" @16:42:47.12 SPR.start /var/log/esp/spr23aug1416h.spr.bz2 @16:42:49.58 Waiting for baseline SPR stabilization @16:42:49.60 Handoff.select :waste @16:42:56.35 Syringe tare is 19% of full scale @16:42:56.38 PinPort.in :locking @16:42:56.49 PinPort.seek :locked @16:42:59.05 Archive.in :fast @16:42:59.16 Lysis2.in :fast @16:42:59.27 Lysis1.seek 1000uL @16:42:59.39 Archive.seek :full @16:42:59.50 Lysis2.seek 1000uL @16:43:04.12 delayUntil "16:52:49.55" @16:52:49.56 Lysis2.in :DAmix @16:52:49.72 Lysis1.in :DAmix @16:52:50.27 Lysis2.seek 750uL @16:52:50.34 Lysis1.seek 750uL @16:53:25.47 delay 5 seconds @16:53:30.49 Handoff.select SPR @16:53:30.65 SPR.command "start 2 14" @16:53:31.30 SPR stabilized @16:53:31.51 delay 10 seconds @16:53:41.53 SPR.command "start 2 0" @16:53:44.67 Waiting up to 11:00 for SPR to enter state 1 @16:55:42.33 SPR.command "hold_on" @16:55:44.25 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:55:44.87 Lysis2.seek 450uL @16:55:44.98 Lysis1.seek 450uL @16:56:27.00 delay 15 seconds @16:56:42.02 SPR.command "hold_off" @16:56:45.32 Waiting up to 38:00 for SPR to enter state 7 @17:11:04.56 SPRsummary:9.59RIU,17.3RIU,112RIU,155RIU after 1792s @17:22:35.64 delay 10 seconds @17:22:45.67 SPR.command "start 2 0" @17:22:48.78 Waiting up to 11:00 for SPR to enter state 1 @17:24:46.45 SPR.command "hold_on" @17:24:47.84 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:24:48.44 Lysis2.seek 300uL @17:24:48.49 Lysis1.seek 300uL @17:25:09.62 delay 15 seconds @17:25:24.65 SPR.command "hold_off" @17:25:27.68 Waiting up to 38:00 for SPR to enter state 7 @17:42:06.97 Session Ended @17:51:18.44 delay 10 seconds @17:51:28.46 SPR.command "start 2 0" @17:51:31.18 Waiting up to 11:00 for SPR to enter state 1 @17:53:28.81 SPR.command "hold_on" @17:53:30.20 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:53:30.87 Lysis2.seek 150uL @17:53:30.92 Lysis1.seek 150uL @17:53:52.02 delay 15 seconds @17:54:07.04 SPR.command "hold_off" @17:54:10.04 Waiting up to 38:00 for SPR to enter state 7 @18:20:00.80 delay 10 seconds @18:20:10.82 SPR.command "start 2 0" @18:20:13.50 Waiting up to 11:00 for SPR to enter state 1 @18:22:11.17 SPR.command "hold_on" @18:22:12.55 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:22:13.16 Lysis2.seek 0uL @18:22:13.22 Lysis1.seek 0uL @18:22:34.30 delay 15 seconds @18:22:49.32 SPR.command "hold_off" @18:22:52.39 Waiting up to 38:00 for SPR to enter state 7 @18:48:43.00 SPR analysis completed @18:48:47.28 Ended log spr23aug1416h.spr.bz2 @18:48:47.30 SPR.command "stats_off" @18:48:47.90 Handoff.select :close @18:48:48.06
Gate.power :heat,:OFF @18:48:48.07 Gate.power :cartridge,:OFF @18:48:48.16 PinPort.in :unlocking @18:48:48.22 PinPort.seek :clear @18:48:50.58 Carousel.in :default @18:48:50.71 Carousel.home.seek 52 @18:48:51.05 Plunge.seek :locked @18:48:51.26 Archive.in :unlocking @18:48:51.42 Archive.seek :clear @18:48:51.51 Air.in :unlocking @18:48:51.68 Air.seek :clear @18:48:51.81 Lysis1.in :unlocking @18:48:51.96 Lysis1.seek :clear @18:48:52.01 Lysis2.in :unlocking @18:48:52.13 Lysis2.seek :clear @18:48:56.73
Gate.power :motor,:OFF @18:48:56.75 Gate.power :core,:OFF @18:48:56.76 Gate.power :analytic1,:OFF @18:48:56.77 Gate.power :analytic2,:OFF @18:48:56.79 Gate.power :main,:OFF @18:48:56.80 Safely stopped and ready to power off @18:48:57.31 Cartridge::None in MAIN -- Not enough standard cartridges remain @23:10:26.83PDT14-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @23:10:28.37 >BREAK< ESP3 Gateway boot v1.43 for core bus @23:10:28.40 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @23:10:31.64 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @23:10:32.22 Sea pressure (Abs) tare is 6% of full scale @23:10:43.18 Polling Can every 2:00 @23:10:43.23 Waiting for espclients... @04:14:10.14PDT15-Aug-23 Session Begun @04:14:11.75 -> can Can@04:14:11, 26.7C, 49% humidity, 14.1psia, 14.870V, 0.088A, 4.351Ah, 1.31W @04:15:18.52 -> exit @04:15:18.56 Session Ended @07:58:10.44 Session Begun @08:00:05.04 -> startDoublet # @08:00:05.33 Gate.power :sampler,:OFF,limit to 3500mA @08:00:05.35 Gate.power :main,:ON,limit to 5000mA @08:00:05.39 Gate.power :analytic1,:ON,limit to 5000mA @08:00:05.42 Gate.power :analytic2,:ON,limit to 5000mA @08:00:05.60 Powering core bus @08:00:07.88 SPR Module 1, FW Rev. B, FPGA Rev. C @08:00:08.36 Archive.configure :default @08:00:08.48 Air.configure :default @08:00:08.57 Lysis1.configure :default @08:00:08.67 Lysis2.configure :default @08:00:08.78 Plunge.configure :default @08:00:08.90 SamplePump.configure :default @08:00:09.02 Toroid.configure :default @08:00:09.16 Carousel.configure :default @08:00:09.29 Pressure.configure :default @08:00:09.41 ExtraPump.configure :default @08:00:09.54 PinPort.configure :default @08:00:09.63 All elf controllers running firmware version 4.84 @08:00:09.66 Gate.power :motor,:ON,limit to 5000mA @08:00:10.01 Archive.home.jog 0 @08:00:11.24 Carousel.home.jog 0 @08:00:11.31 Plunge.home.jog 0 @08:00:11.35 Lysis1.home.jog 0 @08:00:11.38 PinPort.home.jog 0 @08:00:11.43 Lysis2.home.jog 0 @08:00:11.47 Air.home.jog 0 @08:00:11.52 Toroid.home.jog 0 @08:00:12.22 Archive.in :homing @08:00:12.64 Archive.jog 1385 @08:00:12.72 Toroid.in :homing @08:00:12.77 Lysis2.in :homing @08:00:12.83 Air.in :homing @08:00:12.87 PinPort.in :homing @08:00:12.95 Plunge.in :homing @08:00:12.99 Lysis1.in :homing @08:00:13.36 Lysis2.jog 1396 @08:00:13.43 Toroid.jog 600 @08:00:13.49 PinPort.jog -107 @08:00:13.53 Air.jog 1361 @08:00:13.57 Plunge.jog 1569 @08:00:13.63 Lysis1.jog 1389 @08:00:14.05 PinPort.setRawPosition! 0 @08:00:14.13 PinPort.seek :home @08:00:14.26 Archive.setRawPosition! 50 @08:00:14.29 PinPort.in :default @08:00:14.43 Archive.seek :home @08:00:14.75 Archive.in :default @08:00:14.88 Lysis2.setRawPosition! 50 @08:00:14.95 Lysis2.seek :home @08:00:15.10 Lysis1.setRawPosition! 50 @08:00:15.15 Air.setRawPosition! 50 @08:00:15.33 Lysis1.seek :home @08:00:15.35 Air.seek :home @08:00:15.57 Lysis2.in :default @08:00:15.59 Plunge.setRawPosition! 50 @08:00:15.77 Plunge.seek :home @08:00:15.90 Toroid.setRawPosition! 0 @08:00:15.95 Lysis1.in :default @08:00:15.99 Air.in :default @08:00:16.18 Toroid.seek :home @08:00:16.34 Plunge.in :default @08:00:16.37 Toroid.in :default @08:00:16.80 Toroid.seek :clear @08:00:18.44 Carousel.in :default @08:00:18.59 Carousel.home.seek 52 @08:00:18.91 Selecting Cartridge 15 @08:00:19.30 Carousel.seek 15 @08:00:27.24 Lysis1.in :fast @08:00:27.34 Lysis1.seek :locked @08:00:30.01 Plunge.seek :locked @08:00:30.20 Air.in :fast @08:00:30.29 Air.seek :locked @08:00:31.90 Carousel.reconfigure :locked @08:00:32.11 Powering cartridge bus @08:00:35.02 Gate.setIR 1750,5000,0,6 @08:00:35.16 Gate.setIR 0,5000,0,6 @08:00:35.27 Gate.power :cartridge,:OFF @08:00:35.86 Mounted archive Cartridge @08:00:35.99 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 15" @08:00:36.38 Preparing to filter cartridge 15 @08:00:36.72 Gate.power :sampler,:ON @08:00:36.87 Priming sample loop w/150ml, bypass w/50ml @08:00:36.89 SamplePump.setPosition! 0ml @08:00:36.98 Bypass.open @08:00:38.55 Intake.open @08:00:39.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:00:41.77 upsync @08:00:41.98 Vacuum tare is 56% of full scale @08:00:42.19 Pressure.in :default @08:00:42.28 Pressure.hold @08:00:42.55 Waiting for external sampler to finish priming... @08:00:42.57 delayUntil "08:04:06.74" @08:04:06.79 SamplePump.in :prime @08:04:06.90 SamplePump.seek 50ml,2:53 @08:05:07.92 Pressure.stop @08:05:08.05 SamplePump.setPosition! 0ml @08:05:08.14 Exhaust.open @08:05:09.66 Bypass.close @08:05:11.30 Pressure.in :default @08:05:11.40 Pressure.hold @08:05:11.74 SamplePump.in :prime @08:05:11.85 SamplePump.seek 150ml,7:39 @08:08:14.23 Pressure.stop @08:08:14.35 Bypass.open @08:08:15.10 Intake.close @08:08:15.96 Toroid.in :hold @08:08:16.05 Toroid.seek :crack @08:08:17.63 Toroid.seek :cartridge @08:08:18.78 Toroid.in :default @08:08:18.87 Toroid.seek :clear @08:08:21.51 Pressurizing cartridge to 26psi above 14.1psia @08:08:21.53 SamplePump.setPosition! 0ml @08:08:21.62 Intake.open @08:08:22.31 Bypass.close @08:08:23.05 Exhaust.close @08:08:23.93 Pressure.in :default @08:08:24.03 Pressure.hold @08:08:24.31 SamplePump.in :charge @08:08:24.41 SamplePump.seek 2.3ml,42 seconds @08:08:33.77 Pressure.stop @08:08:33.87 Intake.close @08:08:34.64 Pressurized cartridge with 0.392ml to 26.1psi @08:08:34.66 Isolated @35.4psia (21.2psi) @08:08:34.68 Verifying cartridge holds at least 0.65..0.85 of 35.4psia @08:08:34.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:08:39.37 Cartridge pressure fell 2% to 34.7psia @08:08:39.50 Sampling 1000.0ml @08:08:39.52 SamplePump.setPosition! 0ml @08:08:39.61 Exhaust.open @08:08:40.34 Intake.open @08:08:41.24 Pressure.in :default @08:08:41.33 Pressure.hold @08:08:41.51 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:08:41.73 SamplePump.in :sample @08:08:41.84 SamplePump.seek 1000ml,1:23:51 @08:08:44.86 Cartridge 15 filtering 1/1000ml at 0.60ml/s with 28.1psi vacuum @08:09:44.87 Cartridge 15 filtering 33/1000ml at 0.51ml/s with 30.6psi vacuum @08:10:44.87 Cartridge 15 filtering 62/1000ml at 0.50ml/s with 27.6psi vacuum @08:11:44.86 Cartridge 15 filtering 90/1000ml at 0.48ml/s with 21.5psi vacuum @08:12:44.86 Cartridge 15 filtering 116/1000ml at 0.42ml/s with 31.2psi vacuum @08:13:44.86 Cartridge 15 filtering 141/1000ml at 0.42ml/s with 15.3psi vacuum @08:14:44.86 Cartridge 15 filtering 165/1000ml at 0.42ml/s with 29.1psi vacuum @08:15:44.86 Cartridge 15 filtering 188/1000ml at 0.42ml/s with 26.0psi vacuum @08:16:44.88 Cartridge 15 filtering 210/1000ml at 0.29ml/s with 25.9psi vacuum @08:17:44.86 Cartridge 15 filtering 230/1000ml at 0.40ml/s with 21.9psi vacuum @08:18:44.86 Cartridge 15 filtering 250/1000ml at 0.30ml/s with 27.8psi vacuum @08:19:44.86 Cartridge 15 filtering 269/1000ml at 0.27ml/s with 19.6psi vacuum @08:20:44.97 Cartridge 15 filtering 286/1000ml at 0.23ml/s with 28.3psi vacuum @08:21:44.87 Cartridge 15 filtering 303/1000ml at 0.21ml/s with 22.8psi vacuum @08:22:44.87 Cartridge 15 filtering 319/1000ml at 0.34ml/s with 25.5psi vacuum @08:23:44.87 Cartridge 15 filtering 334/1000ml at 0.24ml/s with 30.5psi vacuum @08:24:44.87 Cartridge 15 filtering 348/1000ml at 0.20ml/s with 21.8psi vacuum @08:25:44.87 Cartridge 15 filtering 362/1000ml at 0.20ml/s with 25.1psi vacuum @08:26:13.75 Flow averaged 0.35ml/s over 17:32 @08:26:13.82 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @08:26:14.43 Relieved excess vacuum in .7 seconds @08:26:14.48 SamplePump.seek 1000ml,53:11 @08:26:38.77 Flow averaged 0.21ml/s over 25 seconds @08:26:38.85 Waiting up to 22 seconds for vacuum to reduce from 31.8 to 18.0psi @08:26:39.45 Relieved excess vacuum in .7 seconds @08:26:39.49 SamplePump.seek 1000ml,52:45 @08:26:44.86 Cartridge 15 filtering 374/1000ml at 0.20ml/s with 32.2psi vacuum @08:26:51.38 Flow averaged 0.21ml/s over 12 seconds @08:26:51.46 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @08:26:52.06 Relieved excess vacuum in .7 seconds @08:26:52.10 SamplePump.seek 1000ml,52:33 @08:27:05.31 Flow averaged 0.21ml/s over 14 seconds @08:27:05.39 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @08:27:05.99 Relieved excess vacuum in .7 seconds @08:27:06.03 SamplePump.seek 1000ml,52:19 @08:27:16.43 Flow averaged 0.21ml/s over 11 seconds @08:27:16.51 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi @08:27:17.11 Relieved excess vacuum in .7 seconds @08:27:17.18 SamplePump.seek 1000ml,52:08 @08:27:26.01 Flow estimate reduced to 0.17ml/s @08:27:26.04 Intake.close @08:27:26.16 Exhaust.close @08:27:26.45 Toroid.in :hold @08:27:26.55 Toroid.seek :bypass @08:27:28.23 Toroid.in :default @08:27:28.34 Toroid.seek :clear @08:27:29.98 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:27:30.01 Pressure.stop @08:27:30.31 Sampled 382.4ml @08:27:30.78 GoodNews.email "After filtering 382.4ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 15" @08:27:31.99 Plunge.seek :reagent @08:27:32.86 Sample loop closed. Ready to Deliver Reagents @08:27:32.88 Archive.seek :sealed @08:27:33.46 Gate.power :sampler,:OFF @08:27:36.90 Archive.seek :evac @08:27:37.02 upsync @08:27:50.96 delay 1:00 @08:28:51.00 Archive.seek :clear @08:29:08.50 RNAlater soak for 10:00 @08:39:08.53 Archive.seek :tVent @08:39:11.01 delay 1 second @08:39:12.04 Archive.seek :evac @08:39:27.56 delay 10 seconds @08:39:37.60 Archive.seek :tVent @08:39:53.13 delay 1 second @08:39:54.17 Archive.seek :evac @08:40:09.71 delay 10 seconds @08:40:19.74 Sample preserved and evacuated @08:40:19.91 Selecting Cartridge 14 @08:40:20.19 Carousel.in :default @08:40:20.36 Carousel.home.seek 15 @08:40:20.76 Archive.in :unlocking @08:40:20.94 Archive.seek :clear @08:40:21.09 Air.in :unlocking @08:40:21.30 Air.seek :clear @08:40:25.60 Lysis1.in :unlocking @08:40:25.80 Lysis1.seek :clear @08:40:25.88 Plunge.in :unlocking @08:40:26.03 Plunge.seek :clear @08:40:27.46 Carousel.seek 14 @08:40:28.04 Lysis1.in :fast @08:40:28.14 Lysis1.seek :locked @08:40:30.80 Plunge.in :default @08:40:30.90 Plunge.seek :locked @08:40:31.10 Air.in :fast @08:40:31.24 Air.seek :locked @08:40:32.85 Carousel.reconfigure :locked @08:40:33.04 Powering cartridge bus @08:40:35.71 Gate.setIR 1750,5000,0,6 @08:40:35.84 Gate.setIR 0,5000,0,6 @08:40:35.94 PuckHeater.configure :default @08:40:36.03 Gate.power :heat,:ON,limit to 5000mA @08:40:37.35 Mounted lysis Cartridge board#Q05036 @08:40:37.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:40:37.46 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 14" @08:40:37.86 Preparing to filter cartridge 14 @08:40:38.19 Gate.power :sampler,:ON @08:40:38.36 Priming sample loop w/150ml, bypass w/50ml @08:40:38.38 SamplePump.setPosition! 0ml @08:40:38.50 Bypass.open @08:40:40.05 Intake.open @08:40:40.82 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:40:43.26 upsync @08:40:43.42 Vacuum tare is 56% of full scale @08:40:43.62 Pressure.in :default @08:40:43.72 Pressure.hold @08:40:43.99 Waiting for external sampler to finish priming... @08:40:44.00 delayUntil "08:44:08.21" @08:44:08.27 SamplePump.in :prime @08:44:08.38 SamplePump.seek 50ml,2:53 @08:45:09.39 Pressure.stop @08:45:09.47 SamplePump.setPosition! 0ml @08:45:09.54 Exhaust.open @08:45:11.01 Bypass.close @08:45:12.62 Pressure.in :default @08:45:12.72 Pressure.hold @08:45:13.06 SamplePump.in :prime @08:45:13.19 SamplePump.seek 150ml,7:39 @08:48:15.75 Pressure.stop @08:48:15.91 Bypass.open @08:48:16.67 Intake.close @08:48:17.49 Toroid.in :hold @08:48:17.59 Toroid.seek :crack @08:48:19.15 Toroid.seek :cartridge @08:48:20.32 Toroid.in :default @08:48:20.87 Toroid.seek :clear @08:48:23.01 Pressurizing cartridge to 26psi above 14.2psia @08:48:23.03 SamplePump.setPosition! 0ml @08:48:23.13 Intake.open @08:48:23.82 Bypass.close @08:48:24.57 Exhaust.close @08:48:25.46 Pressure.in :default @08:48:25.55 Pressure.hold @08:48:25.82 SamplePump.in :charge @08:48:25.91 SamplePump.seek 2.3ml,42 seconds @08:48:39.03 Pressure.stop @08:48:39.12 Intake.close @08:48:39.93 Pressurized cartridge with 0.776ml to 26.9psi @08:48:39.95 Isolated @34.7psia (20.5psi) @08:48:39.97 Verifying cartridge holds at least 0.65..0.85 of 34.7psia @08:48:40.02 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:48:44.73 Sampling 50.0ml @08:48:44.75 SamplePump.setPosition! 0ml @08:48:44.84 Exhaust.open @08:48:45.57 Intake.open @08:48:46.45 Pressure.in :default @08:48:46.55 Pressure.hold @08:48:46.72 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:48:46.95 SamplePump.in :sample @08:48:47.04 SamplePump.seek 50ml,4:41 @08:48:50.07 Cartridge 14 filtering 1/50ml at 0.57ml/s with 25.4psi vacuum @08:49:50.08 Cartridge 14 filtering 32/50ml at 0.50ml/s with 22.9psi vacuum @08:50:27.54 Pressure.stop @08:50:27.77 Intake.close @08:50:27.84 Exhaust.close @08:50:28.16 Toroid.in :hold @08:50:28.26 Toroid.seek :bypass @08:50:29.92 Toroid.in :default @08:50:30.02 Toroid.seek :clear @08:50:31.66 Sampled 50.0ml @08:50:32.17 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 14" @08:50:34.43 SPR.command "read_sequences" @08:50:34.51 SPR.command "stats_on" @08:50:34.83 Gate.power :sampler,:OFF @08:50:35.46 SPR.command "start 0 1" @08:50:36.16 SPR.start /var/log/esp/spr23aug1508h.spr.bz2 @08:50:38.20 Handoff.select :waste @08:50:38.24 upsync @08:50:45.01 Syringe tare is 17% of full scale @08:50:45.04 PinPort.in :locking @08:50:45.18 PinPort.seek :locked @08:50:47.76 Archive.in :fast @08:50:47.82 Lysis2.in :fast @08:50:47.90 Lysis1.seek 1350uL @08:50:48.17 Archive.seek :full @08:50:48.22 Lysis2.seek 950uL @08:50:52.99 Plunge.seek :reagent @08:50:53.92 Evacuating puck with filter bubble point of 50psi @08:50:53.95 PinPort.seek :locked @08:50:54.09 Handoff.select :waste @08:51:00.71 Air syringe pumping up to 1 strokes to reach 8psi @08:51:00.72 Air stroke #1 of 1 @08:51:00.86 Air.in :pressurize @08:51:00.95 Air.seek :pressurized @08:51:06.72 1.8psi after pressurize @08:51:06.75 Air.in :compress @08:51:06.86 Air.seek :evac @08:51:09.96 1.7psi after compress @08:51:20.56 0.6psi after compress (stable) @08:51:20.58 Air.in :default @08:51:20.70 Air.hold @08:51:20.96 at 0.4psi Opened to waste @08:51:20.97 Handoff.select :blocked @08:51:21.15 Archive syringe pumping up to 8 strokes to reach 50psi @08:51:21.21 Archive stroke #1 of 8 @08:51:21.36 Archive.in :pressurize @08:51:21.46 Archive.seek :pressurized @08:51:33.97 1.6psi after pressurize @08:51:39.58 1.7psi after pressurize (stable) @08:51:39.61 Archive.in :compress @08:51:39.72 Archive.seek :empty @08:51:54.42 15.2psi after compress @08:52:05.01 16.2psi after compress (stable) @08:52:05.04 Archive.in :gulp @08:52:05.18 Archive.seek :topVent @08:52:11.47 16.3psi after gulp @08:52:17.65 16.3psi after gulp (stable) @08:52:17.66 Archive stroke #2 of 8 @08:52:17.80 Archive.in :pressurize @08:52:17.89 Archive.seek :pressurized @08:52:30.45 16.2psi after pressurize @08:52:36.05 16.1psi after pressurize (stable) @08:52:36.08 Archive.in :compress @08:52:36.19 Archive.seek :empty @08:52:50.84 29.8psi after compress @08:53:01.47 31.0psi after compress (stable) @08:53:01.53 Archive.in :gulp @08:53:01.65 Archive.seek :topVent @08:53:08.45 31.1psi after gulp @08:53:14.05 31.1psi after gulp (stable) @08:53:14.06 Archive stroke #3 of 8 @08:53:14.21 Archive.in :pressurize @08:53:14.30 Archive.seek :pressurized @08:53:26.82 30.9psi after pressurize @08:53:32.43 30.8psi after pressurize (stable) @08:53:32.46 Archive.in :compress @08:53:32.57 Archive.seek :empty @08:53:47.24 43.6psi after compress @08:53:57.85 44.6psi after compress (stable) @08:53:57.87 Archive.in :gulp @08:53:57.98 Archive.seek :topVent @08:54:04.27 44.8psi after gulp @08:54:09.87 44.8psi after gulp (stable) @08:54:09.88 Archive stroke #4 of 8 @08:54:10.03 Archive.in :pressurize @08:54:10.12 Archive.seek :pressurized @08:54:22.62 44.6psi after pressurize @08:54:28.24 44.5psi after pressurize (stable) @08:54:28.27 Archive.in :compress @08:54:28.81 Archive.seek :empty @08:54:38.83 Reached 50.1 of 50.0psi after pumping 6816uL (3.94 strokes) @08:54:38.85 Archive.in :default @08:54:38.99 Archive.hold @08:54:39.09 Handoff.select :waste @08:54:52.37 delay 1:00 @08:55:52.39 Handoff.select :blocked @08:56:15.72 Handoff.select :waste @08:56:15.87 0.6psi at evacEnd @08:56:15.88 Handoff.select :blocked @08:56:16.05 Archive.in :fast @08:56:16.15 Archive.seek :topVent @08:56:29.51 Stable @0.6psi after Handoff blocked @08:56:29.58 Archive syringe pumping up to 8 strokes to reach 31psi @08:56:29.61 Archive stroke #1 of 8 @08:56:29.75 Archive.in :pressurize @08:56:29.86 Archive.seek :pressurized @08:56:42.39 0.9psi after pressurize @08:56:42.41 Archive.in :compress @08:56:42.52 Archive.seek :empty @08:56:57.20 12.8psi after compress @08:57:07.91 13.6psi after compress (stable) @08:57:07.94 Archive.in :gulp @08:57:08.06 Archive.seek :topVent @08:57:14.34 13.7psi after gulp @08:57:19.94 13.6psi after gulp (stable) @08:57:19.96 Archive stroke #2 of 8 @08:57:20.10 Archive.in :pressurize @08:57:20.19 Archive.seek :pressurized @08:57:32.72 13.6psi after pressurize @08:57:32.75 Archive.in :compress @08:57:32.87 Archive.seek :empty @08:57:47.55 25.6psi after compress @08:57:58.16 26.5psi after compress (stable) @08:57:58.19 Archive.in :gulp @08:57:58.31 Archive.seek :topVent @08:58:04.58 26.6psi after gulp @08:58:10.18 26.6psi after gulp (stable) @08:58:10.20 Archive stroke #3 of 8 @08:58:10.34 Archive.in :pressurize @08:58:10.43 Archive.seek :pressurized @08:58:22.95 26.4psi after pressurize @08:58:22.98 Archive.in :compress @08:58:23.14 Archive.seek :empty @08:58:30.26 Reached 30.7 of 30.6psi after pumping 5026uL (2.91 strokes) @08:58:30.28 Archive.in :default @08:58:30.39 Archive.hold @08:58:30.50 Handoff.select :waste @08:58:44.25 delay 1:00 @08:59:44.27 Handoff.select :blocked @09:00:07.02 Handoff.select :waste @09:00:07.20 0.5psi at evacEnd @09:00:07.22 Air.seek :fullVent @09:00:08.69 Archive.in :fast @09:00:08.79 Archive.seek :topVent @09:00:15.35 Seawater evacuated, Pressure above/below filter released. @09:00:15.49 Lysis1.in :slow @09:00:15.61 Lysis1.seek 1050uL @09:00:45.68 delay 5 seconds @09:00:50.71 Handoff.select :blocked @09:00:50.94 Lysis1.seek 450uL @09:01:50.88 delay 3 seconds @09:01:54.02 Air.in mix with 1.40048 parts Lysis1 @09:01:54.77 Air.seek :evac @09:01:54.84 Lysis1.seek 300uL @09:02:10.00 Lysis1.seek :empty @09:02:40.15 Lysis buffer delivered @6.8psi @09:02:40.17 Polling Can every 2:00 @09:02:40.73 PuckHeater.configure :default #ambient 24.8C @09:02:40.81 PuckHeater.seek 79.6C @09:03:31.49 PuckHeater.hold 2:00,79.6C @09:05:31.55 Polling Can every 2:00 @09:05:32.12 PuckHeater.configure :default #ambient 24.8C @09:05:32.21 PuckHeater.hold 30 seconds,78.6C @09:06:02.39 Polling Can every 2:00 @09:06:02.95 PuckHeater.configure :default #ambient 24.8C @09:06:03.03 PuckHeater.hold 30 seconds,78.1C @09:06:33.21 Polling Can every 2:00 @09:06:33.77 PuckHeater.configure :default #ambient 24.8C @09:06:33.86 PuckHeater.hold 10:00,77.6C @09:16:33.08 Lysis complete. Syringe pressure @9.9psi @09:16:33.13 Archive syringe pumping up to 5 strokes to reach 40psi @09:16:33.17 Archive stroke #1 of 5 @09:16:33.32 Archive.in :pressurize @09:16:33.41 Archive.seek :pressurized @09:16:45.98 9.7psi after pressurize @09:16:51.61 9.7psi after pressurize (stable) @09:16:51.65 Archive.in :compress @09:16:51.78 Archive.seek :empty @09:17:05.71 Reached 40.3 of 40.0psi after pumping 1714uL (0.99 strokes) @09:17:05.77 Archive.in :default @09:17:05.89 Archive.hold @09:17:05.99 delay 2 seconds @09:17:08.02 Archive.in :fast @09:17:08.11 Archive.seek :full @09:17:15.34 Lysis1.seek 138uL @09:17:29.23 delay 1 second @09:17:30.27 Archive.seek :full @09:17:30.30 Lysis1.seek :empty @09:17:44.33 Handoff.select :blocked @09:17:44.49 Initial air pressure @40.0psi @09:17:44.61 Lysis1.in :extract @09:17:44.77 Lysis1.seek 600uL,nil @09:17:50.25 Archive syringe pumping up to 6 strokes to reach 25psi @09:17:50.27 Archive stroke #1 of 6 @09:17:50.53 Archive.in :default @09:17:50.60 Archive.hold @09:17:50.81 Initial 600uL lysate @16.1psi @09:17:50.83 Handoff.select :blocked @09:17:50.97 Initial air pressure @16.2psi @09:17:51.05 Lysis1.seek 825uL,nil @09:17:53.09 Archive syringe pumping up to 6 strokes to reach 17psi @09:17:53.12 Archive stroke #1 of 6 @09:17:53.37 Archive.in :pressurize @09:17:53.46 Archive.seek :pressurized @09:18:05.98 13.0psi after pressurize @09:18:16.13 Archive.in :default @09:18:16.27 Archive.hold @09:18:16.62 Recovered 825uL lysate @15.1psi @09:18:16.64 Air.in :halfSpeed @09:18:16.79 Air.seek :fullVent @09:18:19.03 Puck Top Vented, bottom @10.7psi @09:18:19.05 Handoff.select :waste @09:18:19.26 delay 5 seconds @09:18:24.35 Handoff open to waste, bottom @0.4psi @09:18:24.38 Air.in :default @09:18:24.49 Air.seek :evac @09:18:25.95 Lysis2.in :DAmix @09:18:26.11 Lysis1.in :DAmix @09:18:26.86 Lysis2.seek 700uL @09:18:26.90 Lysis1.seek 575uL @09:19:01.99 delay 5 seconds @09:19:07.02 Handoff.select SPR @09:19:07.18 SPR.command "start 2 14" @09:19:08.26 SPR stabilized @09:19:08.46 delay 10 seconds @09:19:18.48 SPR.command "start 2 0" @09:19:21.62 Waiting up to 11:00 for SPR to enter state 1 @09:21:19.27 SPR.command "hold_on" @09:21:20.68 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:21:21.41 Lysis2.seek 400uL @09:21:21.47 Lysis1.seek 275uL @09:22:03.53 delay 15 seconds @09:22:18.55 SPR.command "hold_off" @09:22:21.32 Waiting up to 38:00 for SPR to enter state 7 @09:30:46.06 Session Ended @09:36:41.50 SPRsummary:76.7RIU,1.76e+09RIU,66.9RIU,96.2RIU,none,high,high after 2921s @09:48:11.94 SPR analysis completed @09:48:20.82 Ended log spr23aug1508h.spr.bz2 @09:48:20.84 SPR.command "stats_off" @09:48:21.13 Handoff.select :close @09:48:21.32 Gate.power :heat,:OFF @09:48:21.33 Gate.power :cartridge,:OFF @09:48:21.42 PinPort.in :unlocking @09:48:21.48 PinPort.seek :clear @09:48:23.40 Carousel.in :default @09:48:23.53 Carousel.home.seek 14 @09:48:23.88 Plunge.seek :locked @09:48:24.77 Archive.in :unlocking @09:48:24.92 Archive.seek :clear @09:48:25.01 Air.in :unlocking @09:48:25.20 Air.seek :clear @09:48:25.31 Lysis1.in :unlocking @09:48:25.48 Lysis1.seek :clear @09:48:25.54 Lysis2.in :unlocking @09:48:25.66 Lysis2.seek :clear @09:48:30.03 Gate.power :motor,:OFF @09:48:30.08 Gate.power :core,:OFF @09:48:30.09 Gate.power :analytic1,:OFF @09:48:30.11 Gate.power :analytic2,:OFF @09:48:30.12 Gate.power :main,:OFF @09:48:30.13 Safely stopped and ready to power off @10:23:01.45 Session Begun @10:23:08.07 -> exit @10:23:08.10 Session Ended @10:52:49.30 Session Begun @10:52:53.54 -> Can Can@10:52:36, 24.1C, 50% humidity, 14.2psia, 14.885V, 0.081A, 6.049Ah, 1.21W @10:57:12.22 -> Power {analytic1: false, analytic2: false, cartridge: false, core: false, heat: false, main: false, motor: false, sampler: false} @10:57:21.17 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1508h.spr.bz2}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @11:00:08.02 -> startDoublet # @11:00:08.25 Gate.power :sampler,:OFF,limit to 3500mA @11:00:08.27 Gate.power :main,:ON,limit to 5000mA @11:00:08.30 Gate.power :analytic1,:ON,limit to 5000mA @11:00:08.35 Gate.power :analytic2,:ON,limit to 5000mA @11:00:08.45 Powering core bus @11:00:10.78 SPR Module 1, FW Rev. B, FPGA Rev. C @11:00:11.25 Archive.configure :default @11:00:11.37 Air.configure :default @11:00:11.46 Lysis1.configure :default @11:00:11.55 Lysis2.configure :default @11:00:11.67 Plunge.configure :default @11:00:11.79 SamplePump.configure :default @11:00:11.92 Toroid.configure :default @11:00:12.04 Carousel.configure :default @11:00:12.16 Pressure.configure :default @11:00:12.28 ExtraPump.configure :default @11:00:12.37 PinPort.configure :default @11:00:12.46 All elf controllers running firmware version 4.84 @11:00:12.50 Gate.power :motor,:ON,limit to 5000mA @11:00:12.81 Archive.home.jog 0 @11:00:13.52 Plunge.home.jog 0 @11:00:13.64 Carousel.home.jog 0 @11:00:13.70 Toroid.home.jog 0 @11:00:13.76 Lysis2.home.jog 0 @11:00:13.80 Air.home.jog 0 @11:00:13.85 PinPort.home.jog 0 @11:00:13.90 Lysis1.home.jog 0 @11:00:14.87 Archive.in :homing @11:00:15.13 Archive.jog 1388 @11:00:15.23 Plunge.in :homing @11:00:15.29 Air.in :homing @11:00:15.36 PinPort.in :homing @11:00:15.41 Toroid.in :homing @11:00:15.48 Lysis2.in :homing @11:00:15.52 Lysis1.in :homing @11:00:15.87 Plunge.jog 1574 @11:00:15.93 Air.jog 1371 @11:00:15.98 PinPort.jog -107 @11:00:16.03 Toroid.jog 603 @11:00:16.09 Lysis2.jog 1393 @11:00:16.13 Lysis1.jog 1388 @11:00:17.14 PinPort.setRawPosition! 0 @11:00:17.19 Archive.setRawPosition! 50 @11:00:17.35 PinPort.seek :home @11:00:17.37 Archive.seek :home @11:00:17.66 PinPort.in :default @11:00:17.68 Plunge.setRawPosition! 50 @11:00:17.74 Air.setRawPosition! 50 @11:00:17.94 Air.seek :home @11:00:17.97 Plunge.seek :home @11:00:18.21 Lysis2.setRawPosition! 50 @11:00:18.25 Lysis1.setRawPosition! 50 @11:00:18.28 Archive.in :default @11:00:18.48 Lysis2.seek :home @11:00:18.51 Lysis1.seek :home @11:00:18.73 Plunge.in :default @11:00:18.77 Air.in :default @11:00:19.01 Toroid.setRawPosition! 0 @11:00:19.06 Lysis2.in :default @11:00:19.17 Lysis1.in :default @11:00:19.28 Toroid.seek :home @11:00:19.50 Toroid.in :default @11:00:19.68 Toroid.seek :clear @11:00:21.32 Carousel.in :default @11:00:21.48 Carousel.home.seek 14 @11:00:21.78 Selecting Cartridge 13 @11:00:22.13 Carousel.seek 13 @11:00:22.70 Lysis1.in :fast @11:00:22.80 Lysis1.seek :locked @11:00:25.46 Plunge.seek :locked @11:00:25.65 Air.in :fast @11:00:25.74 Air.seek :locked @11:00:27.34 Carousel.reconfigure :locked @11:00:27.53 Powering cartridge bus @11:00:30.20 Gate.setIR 1750,5000,0,6 @11:00:30.33 Gate.setIR 0,5000,0,6 @11:00:30.44 Gate.power :cartridge,:OFF @11:00:31.01 Mounted archive Cartridge @11:00:31.18 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 13" @11:00:31.52 Preparing to filter cartridge 13 @11:00:31.83 Gate.power :sampler,:ON @11:00:32.00 Priming sample loop w/150ml, bypass w/50ml @11:00:32.02 SamplePump.setPosition! 0ml @11:00:32.14 Bypass.open @11:00:33.86 Intake.open @11:00:34.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:00:37.40 Vacuum tare is 56% of full scale @11:00:37.79 Pressure.in :default @11:00:37.96 Pressure.hold @11:00:38.62 Waiting for external sampler to finish priming... @11:00:38.64 delayUntil "11:04:01.85" @11:00:39.49 upsync @11:04:01.90 SamplePump.in :prime @11:04:02.01 SamplePump.seek 50ml,2:53 @11:05:03.06 Pressure.stop @11:05:03.18 SamplePump.setPosition! 0ml @11:05:03.26 Exhaust.open @11:05:04.75 Bypass.close @11:05:06.36 Pressure.in :default @11:05:06.46 Pressure.hold @11:05:06.81 SamplePump.in :prime @11:05:06.91 SamplePump.seek 150ml,7:39 @11:08:09.35 Pressure.stop @11:08:09.46 Bypass.open @11:08:10.24 Intake.close @11:08:11.06 Toroid.in :hold @11:08:11.17 Toroid.seek :crack @11:08:12.75 Toroid.seek :cartridge @11:08:13.90 Toroid.in :default @11:08:14.00 Toroid.seek :clear @11:08:16.05 Pressurizing cartridge to 26psi above 14.1psia @11:08:16.07 SamplePump.setPosition! 0ml @11:08:16.16 Intake.open @11:08:16.87 Bypass.close @11:08:17.63 Exhaust.close @11:08:18.51 Pressure.in :default @11:08:19.05 Pressure.hold @11:08:19.46 SamplePump.in :charge @11:08:19.56 SamplePump.seek 2.3ml,42 seconds @11:08:28.24 Pressure.stop @11:08:28.33 Intake.close @11:08:29.13 Pressurized cartridge with 0.335ml to 26.3psi @11:08:29.17 Isolated @35.5psia (21.4psi) @11:08:29.22 Verifying cartridge holds at least 0.65..0.85 of 35.5psia @11:08:29.28 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:08:33.89 Cartridge pressure fell 1% to 35.1psia @11:08:34.02 Sampling 1000.0ml @11:08:34.03 SamplePump.setPosition! 0ml @11:08:34.13 Exhaust.open @11:08:34.88 Intake.open @11:08:35.74 Pressure.in :default @11:08:35.83 Pressure.hold @11:08:36.01 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:08:36.23 SamplePump.in :sample @11:08:36.34 SamplePump.seek 1000ml,1:23:51 @11:08:39.35 Cartridge 13 filtering 1/1000ml at 0.59ml/s with 24.3psi vacuum @11:09:39.36 Cartridge 13 filtering 33/1000ml at 0.47ml/s with 18.7psi vacuum @11:10:39.35 Cartridge 13 filtering 61/1000ml at 0.51ml/s with 25.5psi vacuum @11:11:39.36 Cartridge 13 filtering 89/1000ml at 0.43ml/s with 15.1psi vacuum @11:12:39.36 Cartridge 13 filtering 115/1000ml at 0.44ml/s with 25.1psi vacuum @11:13:39.36 Cartridge 13 filtering 140/1000ml at 0.46ml/s with 26.4psi vacuum @11:14:39.36 Cartridge 13 filtering 163/1000ml at 0.36ml/s with 28.7psi vacuum @11:15:39.36 Cartridge 13 filtering 186/1000ml at 0.39ml/s with 14.7psi vacuum @11:16:39.36 Cartridge 13 filtering 208/1000ml at 0.31ml/s with 24.9psi vacuum @11:17:39.36 Cartridge 13 filtering 230/1000ml at 0.30ml/s with 20.6psi vacuum @11:18:39.36 Cartridge 13 filtering 250/1000ml at 0.39ml/s with 23.5psi vacuum @11:19:39.37 Cartridge 13 filtering 270/1000ml at 0.30ml/s with 24.1psi vacuum @11:20:39.36 Cartridge 13 filtering 288/1000ml at 0.29ml/s with 24.8psi vacuum @11:21:39.36 Cartridge 13 filtering 306/1000ml at 0.24ml/s with 23.3psi vacuum @11:22:39.89 Cartridge 13 filtering 322/1000ml at 0.28ml/s with 14.4psi vacuum 4.3m deep (21.0psia) @11:23:39.36 Cartridge 13 filtering 338/1000ml at 0.25ml/s with 17.5psi vacuum @11:24:39.35 Cartridge 13 filtering 354/1000ml at 0.24ml/s with 19.4psi vacuum @11:24:56.09 -> Can Can@11:24:30, 24.6C, 49% humidity, 14.2psia, 14.823V, 0.808A, 6.370Ah, 11.98W @11:25:39.36 Cartridge 13 filtering 368/1000ml at 0.25ml/s with 23.7psi vacuum @11:26:39.37 Cartridge 13 filtering 382/1000ml at 0.21ml/s with 29.7psi vacuum @11:27:39.36 Cartridge 13 filtering 396/1000ml at 0.21ml/s with 21.1psi vacuum @11:28:34.39 Flow averaged 0.34ml/s over 19:59 @11:28:34.46 Waiting up to 15 seconds for vacuum to reduce from 28.9 to 18.0psi @11:28:35.08 Relieved excess vacuum in .7 seconds @11:28:35.15 SamplePump.seek 1000ml,49:53 @11:28:39.35 Cartridge 13 filtering 408/1000ml at 0.20ml/s with 25.1psi vacuum @11:29:05.43 Flow averaged 0.21ml/s over 31 seconds @11:29:05.49 Waiting up to 15 seconds for vacuum to reduce from 28.8 to 18.0psi @11:29:06.16 Relieved excess vacuum in .7 seconds @11:29:06.22 SamplePump.seek 1000ml,49:20 @11:29:19.52 Flow averaged 0.21ml/s over 14 seconds @11:29:19.60 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @11:29:20.21 Relieved excess vacuum in .7 seconds @11:29:20.25 SamplePump.seek 1000ml,49:06 @11:29:29.10 Flow estimate reduced to 0.17ml/s @11:29:29.13 Intake.close @11:29:29.22 Exhaust.close @11:29:29.72 Toroid.in :hold @11:29:29.83 Toroid.seek :bypass @11:29:31.64 Toroid.in :default @11:29:31.75 Toroid.seek :clear @11:29:33.39 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:29:33.42 Pressure.stop @11:29:33.70 Sampled 418.7ml @11:29:34.23 GoodNews.email "After filtering 418.7ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 13" @11:29:34.86 Plunge.seek :reagent @11:29:35.73 Sample loop closed. Ready to Deliver Reagents @11:29:35.76 Archive.seek :sealed @11:29:36.87 Gate.power :sampler,:OFF @11:29:40.33 Archive.seek :evac @11:29:40.45 upsync @11:29:54.44 delay 1:00 @11:30:54.49 Archive.seek :clear @11:31:12.03 RNAlater soak for 10:00 @11:41:12.06 Archive.seek :tVent @11:41:14.54 delay 1 second @11:41:15.57 Archive.seek :evac @11:41:31.12 delay 10 seconds @11:41:41.16 Archive.seek :tVent @11:41:56.74 delay 1 second @11:41:57.77 Archive.seek :evac @11:42:13.32 delay 10 seconds @11:42:23.35 Sample preserved and evacuated @11:42:23.52 Selecting Cartridge 12 @11:42:23.80 Carousel.in :default @11:42:23.97 Carousel.home.seek 13 @11:42:24.37 Archive.in :unlocking @11:42:24.56 Archive.seek :clear @11:42:24.61 Air.in :unlocking @11:42:24.83 Air.seek :clear @11:42:29.24 Lysis1.in :unlocking @11:42:29.40 Lysis1.seek :clear @11:42:29.47 Plunge.in :unlocking @11:42:29.62 Plunge.seek :clear @11:42:31.06 Carousel.seek 12 @11:42:31.64 Lysis1.in :fast @11:42:31.73 Lysis1.seek :locked @11:42:34.40 Plunge.in :default @11:42:34.50 Plunge.seek :locked @11:42:34.69 Air.in :fast @11:42:34.78 Air.seek :locked @11:42:36.39 Carousel.reconfigure :locked @11:42:36.58 Powering cartridge bus @11:42:39.26 Gate.setIR 1750,5000,0,6 @11:42:39.39 Gate.setIR 0,5000,0,6 @11:42:39.49 PuckHeater.configure :default #ambient 24.8C @11:42:39.58 Gate.power :heat,:ON,limit to 5000mA @11:42:40.79 Mounted lysis Cartridge board#Q05062 @11:42:40.85 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:42:40.90 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 12" @11:42:41.36 Preparing to filter cartridge 12 @11:42:41.70 Gate.power :sampler,:ON @11:42:41.82 Priming sample loop w/150ml, bypass w/50ml @11:42:41.87 SamplePump.setPosition! 0ml @11:42:41.96 Bypass.open @11:42:43.53 Intake.open @11:42:44.34 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:42:46.73 upsync @11:42:46.95 Vacuum tare is 56% of full scale @11:42:47.19 Pressure.in :default @11:42:47.29 Pressure.hold @11:42:47.56 Waiting for external sampler to finish priming... @11:42:47.58 delayUntil "11:46:11.72" @11:46:11.78 SamplePump.in :prime @11:46:11.88 SamplePump.seek 50ml,2:53 @11:47:12.90 Pressure.stop @11:47:12.99 SamplePump.setPosition! 0ml @11:47:13.06 Exhaust.open @11:47:14.53 Bypass.close @11:47:16.14 Pressure.in :default @11:47:16.23 Pressure.hold @11:47:16.57 SamplePump.in :prime @11:47:16.68 SamplePump.seek 150ml,7:39 @11:50:19.02 Pressure.stop @11:50:19.15 Bypass.open @11:50:19.94 Intake.close @11:50:20.79 Toroid.in :hold @11:50:20.89 Toroid.seek :crack @11:50:23.03 Toroid.seek :cartridge @11:50:24.19 Toroid.in :default @11:50:24.29 Toroid.seek :clear @11:50:26.36 Pressurizing cartridge to 26psi above 14.1psia @11:50:26.38 SamplePump.setPosition! 0ml @11:50:26.47 Intake.open @11:50:27.20 Bypass.close @11:50:27.94 Exhaust.close @11:50:28.81 Pressure.in :default @11:50:28.91 Pressure.hold @11:50:29.20 SamplePump.in :charge @11:50:29.32 SamplePump.seek 2.3ml,42 seconds @11:50:42.38 Pressure.stop @11:50:42.46 Intake.close @11:50:43.28 Pressurized cartridge with 0.767ml to 26.3psi @11:50:43.30 Isolated @34.2psia (20.2psi) @11:50:43.33 Verifying cartridge holds at least 0.65..0.85 of 34.2psia @11:50:43.38 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:50:48.01 Cartridge pressure fell 1% to 33.9psia @11:50:48.14 Sampling 50.0ml @11:50:48.16 SamplePump.setPosition! 0ml @11:50:48.26 Exhaust.open @11:50:49.03 Intake.open @11:50:49.92 Pressure.in :default @11:50:50.01 Pressure.hold @11:50:50.18 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:50:50.41 SamplePump.in :sample @11:50:50.51 SamplePump.seek 50ml,4:41 @11:50:53.53 Cartridge 12 filtering 1/50ml at 0.49ml/s with 23.6psi vacuum @11:51:53.53 Cartridge 12 filtering 29/50ml at 0.42ml/s with 27.0psi vacuum @11:52:41.45 Pressure.stop @11:52:41.63 Intake.close @11:52:41.70 Exhaust.close @11:52:41.94 Toroid.in :hold @11:52:42.04 Toroid.seek :bypass @11:52:44.20 Toroid.in :default @11:52:44.32 Toroid.seek :clear @11:52:45.96 Sampled 50.0ml @11:52:46.46 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 12" @11:52:48.18 SPR.command "read_sequences" @11:52:48.79 SPR.command "stats_on" @11:52:49.03 SPR.command "start 0 1" @11:52:49.13 Gate.power :sampler,:OFF @11:52:49.71 SPR.start /var/log/esp/spr23aug1511h.spr.bz2 @11:52:51.65 Handoff.select :waste @11:52:52.63 upsync @11:52:58.42 Syringe tare is 18% of full scale @11:52:58.46 PinPort.in :locking @11:52:58.63 PinPort.seek :locked @11:53:01.28 Archive.in :fast @11:53:01.43 Lysis2.in :fast @11:53:01.48 Lysis1.seek 1350uL @11:53:01.54 Archive.seek :full @11:53:01.81 Lysis2.seek 950uL @11:53:06.55 Plunge.seek :reagent @11:53:07.50 Evacuating puck with filter bubble point of 50psi @11:53:07.53 PinPort.seek :locked @11:53:08.21 Handoff.select :waste @11:53:14.86 Air syringe pumping up to 1 strokes to reach 8psi @11:53:14.89 Air stroke #1 of 1 @11:53:15.04 Air.in :pressurize @11:53:15.17 Air.seek :pressurized @11:53:20.95 2.2psi after pressurize @11:53:20.98 Air.in :compress @11:53:21.10 Air.seek :evac @11:53:24.19 2.5psi after compress @11:53:36.80 0.6psi after compress (stable) @11:53:36.81 Air.in :default @11:53:36.92 Air.hold @11:53:37.77 at 0.4psi Opened to waste @11:53:37.79 Handoff.select :blocked @11:53:37.97 Archive syringe pumping up to 8 strokes to reach 50psi @11:53:37.98 Archive stroke #1 of 8 @11:53:38.12 Archive.in :pressurize @11:53:38.22 Archive.seek :pressurized @11:53:50.77 0.7psi after pressurize @11:53:56.43 0.7psi after pressurize (stable) @11:53:56.49 Archive.in :compress @11:53:56.60 Archive.seek :empty @11:54:11.28 17.5psi after compress @11:54:22.02 18.9psi after compress (stable) @11:54:22.05 Archive.in :gulp @11:54:22.17 Archive.seek :topVent @11:54:28.46 19.5psi after gulp @11:54:34.07 19.7psi after gulp (stable) @11:54:34.08 Archive stroke #2 of 8 @11:54:34.22 Archive.in :pressurize @11:54:34.37 Archive.seek :pressurized @11:54:46.90 19.6psi after pressurize @11:54:52.50 19.6psi after pressurize (stable) @11:54:52.53 Archive.in :compress @11:54:52.64 Archive.seek :empty @11:55:07.29 33.3psi after compress @11:55:17.89 34.9psi after compress (stable) @11:55:17.92 Archive.in :gulp @11:55:18.03 Archive.seek :topVent @11:55:24.31 35.2psi after gulp @11:55:29.91 35.3psi after gulp (stable) @11:55:29.93 Archive stroke #3 of 8 @11:55:30.10 Archive.in :pressurize @11:55:30.22 Archive.seek :pressurized @11:55:42.74 35.2psi after pressurize @11:55:48.34 35.1psi after pressurize (stable) @11:55:48.37 Archive.in :compress @11:55:48.48 Archive.seek :empty @11:56:03.19 49.4psi after compress @11:56:03.80 Reached 50.1 of 50.0psi after pumping 5190uL (3.00 strokes) @11:56:03.82 Archive.in :default @11:56:03.94 Archive.hold @11:56:04.04 Handoff.select :waste @11:56:13.84 delay 1:00 @11:57:13.86 Handoff.select :blocked @11:57:36.65 Handoff.select :waste @11:57:36.81 0.2psi at evacEnd @11:57:36.83 Handoff.select :blocked @11:57:37.00 Archive.in :fast @11:57:37.09 Archive.seek :topVent @11:57:50.84 Stable @0.5psi after Handoff blocked @11:57:50.89 Archive syringe pumping up to 8 strokes to reach 31psi @11:57:50.91 Archive stroke #1 of 8 @11:57:51.08 Archive.in :pressurize @11:57:51.22 Archive.seek :pressurized @11:58:03.75 0.6psi after pressurize @11:58:03.79 Archive.in :compress @11:58:03.90 Archive.seek :empty @11:58:18.55 14.2psi after compress @11:58:29.19 15.3psi after compress (stable) @11:58:29.22 Archive.in :gulp @11:58:29.34 Archive.seek :topVent @11:58:35.62 15.5psi after gulp @11:58:41.25 15.5psi after gulp (stable) @11:58:41.26 Archive stroke #2 of 8 @11:58:41.41 Archive.in :pressurize @11:58:41.51 Archive.seek :pressurized @11:58:54.04 15.4psi after pressurize @11:58:54.07 Archive.in :compress @11:58:54.19 Archive.seek :empty @11:59:08.83 29.1psi after compress @11:59:19.44 30.2psi after compress (stable) @11:59:19.47 Archive.in :gulp @11:59:19.64 Archive.seek :topVent @11:59:25.98 30.5psi after gulp @11:59:31.58 30.4psi after gulp (stable) @11:59:31.60 Archive stroke #3 of 8 @11:59:31.74 Archive.in :pressurize @11:59:31.84 Archive.seek :pressurized @11:59:32.09 Reached 30.5 of 30.5psi after pumping 3461uL (2.00 strokes) @11:59:32.11 Archive.in :default @11:59:32.28 Archive.hold @11:59:32.38 Handoff.select :waste @11:59:39.62 delay 1:00 @12:00:39.64 Handoff.select :blocked @12:01:02.37 Handoff.select :waste @12:01:02.53 0.4psi at evacEnd @12:01:02.55 Air.seek :fullVent @12:01:04.01 Archive.in :fast @12:01:04.11 Archive.seek :topVent @12:01:04.30 Seawater evacuated, Pressure above/below filter released. @12:01:04.43 Lysis1.in :slow @12:01:04.52 Lysis1.seek 1050uL @12:01:34.56 delay 5 seconds @12:01:39.58 Handoff.select :blocked @12:01:39.85 Lysis1.seek 450uL @12:02:39.82 delay 3 seconds @12:02:42.93 Air.in mix with 1.40048 parts Lysis1 @12:02:43.69 Air.seek :evac @12:02:43.80 Lysis1.seek 300uL @12:02:58.89 Lysis1.seek :empty @12:03:29.02 Lysis buffer delivered @42.0psi @12:03:29.04 Polling Can every 2:00 @12:03:29.63 PuckHeater.configure :default #ambient 25.2C @12:03:29.75 PuckHeater.seek 79.6C @12:04:28.07 PuckHeater.hold 2:00,79.6C @12:06:28.14 Polling Can every 2:00 @12:06:28.71 PuckHeater.configure :default #ambient 25.3C @12:06:28.79 PuckHeater.hold 30 seconds,78.6C @12:06:58.97 Polling Can every 2:00 @12:06:59.53 PuckHeater.configure :default #ambient 25.3C @12:06:59.66 PuckHeater.hold 30 seconds,78.1C @12:07:29.86 Polling Can every 2:00 @12:07:30.42 PuckHeater.configure :default #ambient 25.3C @12:07:30.51 PuckHeater.hold 10:00,77.5C @12:17:29.99 Lysis complete. Syringe pressure @51.4psi @12:17:30.04 Archive syringe pumping up to 5 strokes to reach 40psi @12:17:30.06 Archive stroke #1 of 5 @12:17:30.19 Archive.in :pressurize @12:17:30.29 Archive.seek :pressurized @12:17:30.55 Reached 51.4 of 40.0psi after pumping 1uL (0.00 strokes) @12:17:30.57 Archive.in :default @12:17:30.69 Archive.hold @12:17:30.80 delay 2 seconds @12:17:32.83 Archive.in :fast @12:17:32.92 Archive.seek :full @12:17:33.22 Lysis1.seek 138uL @12:17:47.15 delay 1 second @12:17:48.20 Archive.seek :full @12:17:48.24 Lysis1.seek :empty @12:18:02.21 Handoff.select :blocked @12:18:02.36 Initial air pressure @50.3psi @12:18:03.00 Lysis1.in :extract @12:18:03.20 Lysis1.seek 600uL,nil @12:18:04.98 Archive syringe pumping up to 6 strokes to reach 25psi @12:18:04.99 Archive stroke #1 of 6 @12:18:05.24 Archive.in :pressurize @12:18:05.36 Archive.seek :pressurized @12:18:17.94 10.9psi after pressurize @12:18:23.59 10.9psi after pressurize (stable) @12:18:23.62 Archive.in :compress @12:18:23.73 Archive.seek :empty @12:18:33.27 Archive.in :default @12:18:33.35 Archive.hold @12:18:33.55 Initial 600uL lysate @17.9psi @12:18:33.57 Handoff.select :blocked @12:18:33.72 Initial air pressure @19.2psi @12:18:33.83 Lysis1.seek 825uL,nil @12:18:35.71 Archive syringe pumping up to 6 strokes to reach 17psi @12:18:35.77 Archive stroke #1 of 6 @12:18:35.98 Archive.in :compress @12:18:36.08 Archive.seek :empty @12:18:37.45 Archive.in :default @12:18:37.53 Archive.hold @12:18:37.88 Recovered 825uL lysate @15.7psi @12:18:37.90 Air.in :halfSpeed @12:18:38.02 Air.seek :fullVent @12:18:40.27 Puck Top Vented, bottom @3.9psi @12:18:40.29 Handoff.select :waste @12:18:40.46 delay 5 seconds @12:18:45.55 Handoff open to waste, bottom @0.3psi @12:18:45.58 Air.in :default @12:18:45.69 Air.seek :evac @12:18:47.14 Lysis2.in :DAmix @12:18:47.33 Lysis1.in :DAmix @12:18:47.97 Lysis2.seek 700uL @12:18:48.05 Lysis1.seek 575uL @12:19:23.23 delay 5 seconds @12:19:28.25 Handoff.select SPR @12:19:28.41 SPR.command "start 2 14" @12:19:29.19 SPR stabilized @12:19:29.40 delay 10 seconds @12:19:39.42 SPR.command "start 2 0" @12:19:42.53 Waiting up to 11:00 for SPR to enter state 1 @12:21:40.18 SPR.command "hold_on" @12:21:41.57 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:21:42.27 Lysis2.seek 400uL @12:21:42.35 Lysis1.seek 275uL @12:22:24.39 delay 15 seconds @12:22:39.41 SPR.command "hold_off" @12:22:42.24 Waiting up to 38:00 for SPR to enter state 7 @12:37:02.41 SPRsummary:4.43RIU,-2.95e+03RIU,73.8RIU,91.2RIU,high,240ng/L,375ng/L after 2802s @12:45:04.27 Session Ended @12:48:32.84 SPR analysis completed @12:48:40.51 Ended log spr23aug1511h.spr.bz2 @12:48:40.52 SPR.command "stats_off" @12:48:41.02 Handoff.select :close @12:48:41.19 Gate.power :heat,:OFF @12:48:41.21 Gate.power :cartridge,:OFF @12:48:41.30 PinPort.in :unlocking @12:48:41.36 PinPort.seek :clear @12:48:43.28 Carousel.in :default @12:48:43.42 Carousel.home.seek 12 @12:48:43.75 Plunge.seek :locked @12:48:44.65 Archive.in :unlocking @12:48:44.82 Archive.seek :clear @12:48:44.91 Air.in :unlocking @12:48:45.07 Air.seek :clear @12:48:45.22 Lysis1.in :unlocking @12:48:45.36 Lysis1.seek :clear @12:48:45.42 Lysis2.in :unlocking @12:48:45.55 Lysis2.seek :clear @12:48:49.51 Gate.power :motor,:OFF @12:48:49.53 Gate.power :core,:OFF @12:48:49.55 Gate.power :analytic1,:OFF @12:48:49.56 Gate.power :analytic2,:OFF @12:48:49.57 Gate.power :main,:OFF @12:48:49.59 Safely stopped and ready to power off @15:22:55.24 Session Begun @15:23:00.30 -> showlog 20 @15:23:24.16 -> exit @15:23:24.18 Session Ended @16:01:20.23 Session Begun @16:01:22.57 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [10,8,6] => { Type: :microSPR_phyto, State: :dry}, [11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1511h.spr.bz2}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1508h.spr.bz2}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @16:11:52.75 -> doubleRegen @16:11:52.82 Gate.power :sampler,:OFF,limit to 3500mA @16:11:52.83 Gate.power :main,:ON,limit to 5000mA @16:11:52.87 Gate.power :analytic1,:ON,limit to 5000mA @16:11:52.91 Gate.power :analytic2,:ON,limit to 5000mA @16:11:53.01 Powering core bus @16:11:55.35 SPR Module 1, FW Rev. B, FPGA Rev. C @16:11:55.78 Archive.configure :default @16:11:55.90 Air.configure :default @16:11:56.00 Lysis1.configure :default @16:11:56.09 Lysis2.configure :default @16:11:56.21 Plunge.configure :default @16:11:56.34 SamplePump.configure :default @16:11:56.47 Toroid.configure :default @16:11:56.59 Carousel.configure :default @16:11:56.71 Pressure.configure :default @16:11:56.83 ExtraPump.configure :default @16:11:56.92 PinPort.configure :default @16:11:57.01 All elf controllers running firmware version 4.84 @16:11:57.04 Gate.power :motor,:ON,limit to 5000mA @16:11:57.33 Archive.home.jog 0 @16:11:58.17 Carousel.home.jog 0 @16:11:58.22 Plunge.home.jog 0 @16:11:58.28 Lysis2.home.jog 0 @16:11:58.32 Air.home.jog 0 @16:11:58.36 PinPort.home.jog 0 @16:11:58.41 Toroid.home.jog 0 @16:11:58.45 Lysis1.home.jog 0 @16:11:59.37 Archive.in :homing @16:11:59.69 Archive.jog 1390 @16:11:59.77 Toroid.in :homing @16:11:59.82 Lysis2.in :homing @16:11:59.86 Air.in :homing @16:11:59.94 Plunge.in :homing @16:11:59.99 Lysis1.in :homing @16:12:00.03 PinPort.in :homing @16:12:00.37 Lysis2.jog 1396 @16:12:00.44 Toroid.jog 599 @16:12:00.52 Air.jog 1369 @16:12:00.62 PinPort.jog -106 @16:12:00.66 Plunge.jog 1574 @16:12:00.72 Lysis1.jog 1389 @16:12:01.66 PinPort.setRawPosition! 0 @16:12:01.68 Archive.setRawPosition! 50 @16:12:01.79 PinPort.seek :home @16:12:01.88 Archive.seek :home @16:12:02.15 Lysis2.setRawPosition! 50 @16:12:02.21 Lysis1.setRawPosition! 50 @16:12:02.26 PinPort.in :default @16:12:02.29 Air.setRawPosition! 50 @16:12:02.51 Lysis2.seek :home @16:12:02.56 Lysis1.seek :home @16:12:02.60 Air.seek :home @16:12:02.90 Toroid.setRawPosition! 0 @16:12:02.96 Plunge.setRawPosition! 50 @16:12:02.99 Archive.in :default @16:12:03.24 Plunge.seek :home @16:12:03.27 Toroid.seek :home @16:12:03.54 Lysis2.in :default @16:12:03.58 Lysis1.in :default @16:12:03.66 Toroid.in :default @16:12:03.70 Air.in :default @16:12:04.00 Plunge.in :default @16:12:04.36 Toroid.seek :clear @16:12:06.00 Carousel.in :default @16:12:06.16 Carousel.home.seek 12 @16:12:07.57 SPR.command "read_sequences" @16:12:07.65 SPR.command "stats_on" @16:12:08.47 delay 2 seconds @16:12:09.27 SPR.start /var/log/esp/spr23aug1516h.spr.bz2 @16:12:10.49 SPR.command "start 3 0" @16:12:11.32 delay 15:00 @16:27:11.34 SPR.command "start 2 14" @16:27:18.18 Ended log spr23aug1516h.spr.bz2 @16:27:18.20 SPR.command "stats_off" @16:27:18.36 delay 10 seconds @16:27:28.55 Carousel.in :default @16:27:28.71 Carousel.home.seek 12 @16:27:29.09 Lysis1.in :fast @16:27:29.22 Lysis1.seek :locked @16:27:31.76 Plunge.seek :locked @16:27:33.15 Lysis1.in :unlocking @16:27:33.35 Lysis1.seek :clear @16:27:34.84 Gate.power :motor,:OFF @16:27:34.86 Gate.power :core,:OFF @16:27:34.90 Gate.power :analytic1,:OFF @16:27:34.94 Gate.power :analytic2,:OFF @16:27:34.97 Gate.power :main,:OFF @16:27:35.00 Safely stopped and ready to power off @16:28:24.66 -> Cartridge.release 51 1 @16:28:46.43 -> MainThread.exit # @16:28:46.56 Session Ended @16:29:23.69PDT15-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @16:29:25.34 >BREAK< ESP3 Gateway boot v1.43 for core bus @16:29:25.37 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @16:29:28.73 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @16:29:29.36 Sea pressure (Abs) tare is 6% of full scale @16:29:40.62 Polling Can every 2:00 @16:29:40.67 Loading /home/esp/esp2/bin/processAll mission @16:29:40.88 Sea pressure (Abs) tare is 6% of full scale @16:29:40.89 Sampling default goal volumes @16:29:40.91 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @16:29:40.97 Gate.power :sampler,:OFF,limit to 3500mA @16:29:40.98 Gate.power :main,:ON,limit to 5000mA @16:29:41.00 Gate.power :analytic1,:ON,limit to 5000mA @16:29:41.01 Gate.power :analytic2,:ON,limit to 5000mA @16:29:41.21 Powering core bus @16:29:43.43 SPR Module 1, FW Rev. B, FPGA Rev. C @16:29:43.97
Archive.configure :default @16:29:44.07 Air.configure :default @16:29:44.13 Lysis1.configure :default @16:29:44.19 Lysis2.configure :default @16:29:44.28 Plunge.configure :default @16:29:44.37 SamplePump.configure :default @16:29:44.46 Toroid.configure :default @16:29:44.56 Carousel.configure :default @16:29:44.65 Pressure.configure :default @16:29:44.74 ExtraPump.configure :default @16:29:44.80 PinPort.configure :default @16:29:44.86 All elf controllers running firmware version 4.84 @16:29:44.89 Gate.power :motor,:ON,limit to 5000mA @16:29:45.12 Archive.home.jog 0 @16:29:45.91 PinPort.home.jog 0 @16:29:45.95 Carousel.home.jog 0 @16:29:46.00 Plunge.home.jog 0 @16:29:46.02 Lysis2.home.jog 0 @16:29:46.04 Air.home.jog 0 @16:29:46.06 Toroid.home.jog 0 @16:29:46.09 Lysis1.home.jog 0 @16:29:46.85 Archive.in :homing @16:29:47.12 Archive.jog 1387 @16:29:47.23 Toroid.in :homing @16:29:47.26 Lysis2.in :homing @16:29:47.29 PinPort.in :homing @16:29:47.35 Plunge.in :homing @16:29:47.38 Air.in :homing @16:29:47.43 Lysis1.in :homing @16:29:47.68 Toroid.jog 594 @16:29:47.79 PinPort.jog -107 @16:29:47.88 Lysis2.jog 1398 @16:29:47.91 Air.jog 1366 @16:29:48.43 Plunge.jog 1573 @16:29:48.46 Lysis1.jog 1389 @16:29:48.80 PinPort.setRawPosition! 0 @16:29:48.82 Archive.setRawPosition! 50 @16:29:48.90 PinPort.seek :home @16:29:48.95 Archive.seek :home @16:29:49.10 PinPort.in :default @16:29:49.30 Archive.in :default @16:29:49.70 Lysis2.setRawPosition! 50 @16:29:49.76 Lysis2.seek :home @16:29:49.82 Air.setRawPosition! 50 @16:29:49.93 Air.seek :home @16:29:50.02 Plunge.setRawPosition! 50 @16:29:50.04 Lysis1.setRawPosition! 50 @16:29:50.15 Plunge.seek :home @16:29:50.19 Lysis1.seek :home @16:29:50.36 Lysis2.in :default @16:29:50.40 Air.in :default @16:29:50.43 Toroid.setRawPosition! 0 @16:29:50.61 Toroid.seek :home @16:29:50.73 Plunge.in :default @16:29:50.77 Lysis1.in :default @16:29:50.82 Toroid.in :default @16:29:51.24
Toroid.seek :clear @16:29:52.86 Carousel.in :default @16:29:52.98 Carousel.home.seek 12 @16:29:53.29 Selecting Cartridge 51 @16:29:53.60 Carousel.seek 51 @16:30:01.89 Lysis1.in :fast @16:30:01.95 Lysis1.seek :locked @16:30:04.57 Plunge.seek :locked @16:30:04.73 Air.in :fast @16:30:04.80 Air.seek :locked @16:30:06.37
Carousel.reconfigure :locked @16:30:06.54 Powering cartridge bus @16:30:09.23 Gate.setIR 1750,5000,0,6 @16:30:09.35 Gate.setIR 0,5000,0,6 @16:30:09.42 PuckHeater.configure :default @16:30:09.48 Gate.power :heat,:ON,limit to 5000mA @16:30:10.15 Mounted lysis Cartridge @16:30:10.17 WARNING: Using default puck temperature sensor calibration @16:30:10.19 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:30:11.76 SPR.command "read_sequences" @16:30:11.82 SPR.command "stats_on" @16:30:11.97 SPR.command "start 0 1" @16:30:12.68 SPR.start /var/log/esp/spr23aug1516h#2.spr.bz2 @16:30:14.54 Waiting for baseline SPR stabilization @16:30:14.56 Handoff.select :waste @16:30:21.97 Syringe tare is 18% of full scale @16:30:22.00 PinPort.in :locking @16:30:22.06 PinPort.seek :locked @16:30:24.60 Archive.in :fast @16:30:24.67 Lysis2.in :fast @16:30:24.71 Lysis1.seek 1000uL @16:30:24.90 Archive.seek :full @16:30:24.97 Lysis2.seek 1000uL @16:30:29.51 delayUntil "16:40:14.08" @16:40:14.09 Lysis1.in :DAmix @16:40:14.22 Lysis2.in :DAmix @16:40:14.74 Lysis1.seek 750uL @16:40:14.81 Lysis2.seek 750uL @16:40:49.89 delay 5 seconds @16:40:54.91 Handoff.select SPR @16:40:55.04 SPR.command "start 2 14" @16:40:56.31 SPR stabilized @16:40:56.46 delay 10 seconds @16:41:06.49 SPR.command "start 2 0" @16:41:09.19 Waiting up to 11:00 for SPR to enter state 1 @16:43:06.83 SPR.command "hold_on" @16:43:08.24 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:43:08.84 Lysis1.seek 450uL @16:43:08.91 Lysis2.seek 450uL @16:43:50.91 delay 15 seconds @16:44:05.93 SPR.command "hold_off" @16:44:09.38 Waiting up to 38:00 for SPR to enter state 7 @16:58:29.99 SPRsummary:-3.3RIU,9.93RIU,107RIU,153RIU after 1792s @17:10:00.57 delay 10 seconds @17:10:10.59 SPR.command "start 2 0" @17:10:13.30 Waiting up to 11:00 for SPR to enter state 1 @17:12:10.94 SPR.command "hold_on" @17:12:12.85 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:12:13.45 Lysis1.seek 300uL @17:12:13.50 Lysis2.seek 300uL @17:12:34.61 delay 15 seconds @17:12:49.63 SPR.command "hold_off" @17:12:52.20 Waiting up to 38:00 for SPR to enter state 7 @17:38:42.00 delay 10 seconds @17:38:52.02 SPR.command "start 2 0" @17:38:55.67 Waiting up to 11:00 for SPR to enter state 1 @17:40:53.31 SPR.command "hold_on" @17:40:55.24 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:40:55.76 Lysis1.seek 150uL @17:40:55.81 Lysis2.seek 150uL @17:41:16.91 delay 15 seconds @17:41:31.95 SPR.command "hold_off" @17:41:34.56 Waiting up to 38:00 for SPR to enter state 7 @18:07:24.37 delay 10 seconds @18:07:34.39 SPR.command "start 2 0" @18:07:37.08 Waiting up to 11:00 for SPR to enter state 1 @18:09:34.74 SPR.command "hold_on" @18:09:36.65 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:09:37.23 Lysis1.seek 0uL @18:09:37.32 Lysis2.seek 0uL @18:09:58.37 delay 15 seconds @18:10:13.40 SPR.command "hold_off" @18:10:15.98 Waiting up to 38:00 for SPR to enter state 7 @18:36:05.64 SPR analysis completed @18:36:09.80 Ended log spr23aug1516h#2.spr.bz2 @18:36:09.81 SPR.command "stats_off" @18:36:10.54 Handoff.select :close @18:36:10.70
Gate.power :heat,:OFF @18:36:10.71 Gate.power :cartridge,:OFF @18:36:10.79 PinPort.in :unlocking @18:36:10.86 PinPort.seek :clear @18:36:13.24 Carousel.in :default @18:36:13.38 Carousel.home.seek 51 @18:36:13.73 Plunge.seek :locked @18:36:13.90 Archive.in :unlocking @18:36:14.08 Archive.seek :clear @18:36:14.16 Air.in :unlocking @18:36:14.34 Air.seek :clear @18:36:14.41 Lysis1.in :unlocking @18:36:14.59 Lysis1.seek :clear @18:36:14.71 Lysis2.in :unlocking @18:36:14.80 Lysis2.seek :clear @18:36:19.40
Gate.power :motor,:OFF @18:36:19.42 Gate.power :core,:OFF @18:36:19.43 Gate.power :analytic1,:OFF @18:36:19.44 Gate.power :analytic2,:OFF @18:36:19.46 Gate.power :main,:OFF @18:36:19.47 Safely stopped and ready to power off @18:36:19.98 Cartridge::None in MAIN -- Not enough standard cartridges remain @21:09:51.01PDT15-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @21:09:52.63 >BREAK< ESP3 Gateway boot v1.43 for core bus @21:09:52.66 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @21:09:55.97 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @21:09:56.57 Sea pressure (Abs) tare is 6% of full scale @21:10:09.42 Polling Can every 2:00 @21:10:09.48 Waiting for espclients... @21:10:15.04 Session Begun @21:10:16.94 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [10,8,6] => { Type: :microSPR_phyto, State: :dry}, [11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @21:10:53.61 -> exit @21:10:53.63 Session Ended @03:18:17.36PDT16-Aug-23 Session Begun @03:18:49.88 -> fireDoublet :fireDoublet @03:19:05.35 -> starDoublet :starDoublet @03:19:18.62 -> startDoublet # @03:19:18.87 Gate.power :sampler,:OFF,limit to 3500mA @03:19:18.89 Gate.power :main,:ON,limit to 5000mA @03:19:18.93 Gate.power :analytic1,:ON,limit to 5000mA @03:19:18.97 Gate.power :analytic2,:ON,limit to 5000mA @03:19:19.16 Powering core bus @03:19:21.41 SPR Module 1, FW Rev. B, FPGA Rev. C @03:19:21.94 Archive.configure :default @03:19:22.06 Air.configure :default @03:19:22.15 Lysis1.configure :default @03:19:22.24 Lysis2.configure :default @03:19:22.36 Plunge.configure :default @03:19:22.48 SamplePump.configure :default @03:19:22.61 Toroid.configure :default @03:19:22.74 Carousel.configure :default @03:19:22.86 Pressure.configure :default @03:19:22.98 ExtraPump.configure :default @03:19:23.07 PinPort.configure :default @03:19:23.19 All elf controllers running firmware version 4.84 @03:19:23.24 Gate.power :motor,:ON,limit to 5000mA @03:19:23.60 Archive.home.jog 0 @03:19:24.70 Carousel.home.jog 0 @03:19:24.85 Plunge.home.jog 0 @03:19:24.90 Lysis2.home.jog 0 @03:19:24.94 PinPort.home.jog 0 @03:19:24.98 Toroid.home.jog 0 @03:19:25.02 Air.home.jog 0 @03:19:25.06 Lysis1.home.jog 0 @03:19:25.84 Archive.in :homing @03:19:26.21 Toroid.in :homing @03:19:26.26 Lysis2.in :homing @03:19:26.33 Archive.jog 1383 @03:19:26.37 PinPort.in :homing @03:19:26.43 Plunge.in :homing @03:19:26.70 Toroid.jog 597 @03:19:26.76 Lysis2.jog 1394 @03:19:26.80 PinPort.jog -108 @03:19:26.86 Plunge.jog 1572 @03:19:27.23 Air.in :homing @03:19:27.27 Lysis1.in :homing @03:19:27.42 Air.jog 1361 @03:19:27.47 Lysis1.jog 1390 @03:19:27.65 PinPort.setRawPosition! 0 @03:19:27.73 PinPort.seek :home @03:19:27.83 Archive.setRawPosition! 50 @03:19:27.94 Archive.seek :home @03:19:28.06 PinPort.in :default @03:19:28.18 Lysis2.setRawPosition! 50 @03:19:28.26 Lysis2.seek :home @03:19:28.40 Archive.in :default @03:19:28.42 Plunge.setRawPosition! 50 @03:19:28.60 Plunge.seek :home @03:19:28.76 Lysis1.setRawPosition! 50 @03:19:28.79 Air.setRawPosition! 50 @03:19:28.83 Lysis2.in :default @03:19:29.03 Lysis1.seek :home @03:19:29.08 Air.seek :home @03:19:29.35 Plunge.in :default @03:19:29.38 Toroid.setRawPosition! 0 @03:19:29.54 Toroid.seek :home @03:19:29.71 Toroid.in :default @03:19:29.74 Lysis1.in :default @03:19:29.82 Air.in :default @03:19:30.27 Toroid.seek :clear @03:19:31.92 Carousel.in :default @03:19:32.08 Carousel.home.seek 51 @03:19:32.39 Selecting Cartridge 11 @03:19:32.75 Carousel.seek 11 @03:19:41.31 Lysis1.in :fast @03:19:41.40 Lysis1.seek :locked @03:19:44.05 Plunge.seek :locked @03:19:44.26 Air.in :fast @03:19:44.36 Air.seek :locked @03:19:45.96 Carousel.reconfigure :locked @03:19:46.17 Powering cartridge bus @03:19:48.83 Gate.setIR 1750,5000,0,6 @03:19:48.96 Gate.setIR 0,5000,0,6 @03:19:49.06 Gate.power :cartridge,:OFF @03:19:50.03 Mounted archive Cartridge @03:19:50.22 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 11" @03:19:50.66 Preparing to filter cartridge 11 @03:19:51.01 Gate.power :sampler,:ON @03:19:51.20 Priming sample loop w/150ml, bypass w/50ml @03:19:51.24 SamplePump.setPosition! 0ml @03:19:51.33 Bypass.open @03:19:52.90 Intake.open @03:19:53.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:19:55.88 upsync @03:19:56.32 Vacuum tare is 56% of full scale @03:19:56.52 Pressure.in :default @03:19:56.62 Pressure.hold @03:19:56.89 Waiting for external sampler to finish priming... @03:19:56.91 delayUntil "03:23:21.04" @03:23:21.10 SamplePump.in :prime @03:23:21.24 SamplePump.seek 50ml,2:53 @03:24:22.27 Pressure.stop @03:24:22.36 SamplePump.setPosition! 0ml @03:24:22.44 Exhaust.open @03:24:23.92 Bypass.close @03:24:25.53 Pressure.in :default @03:24:25.63 Pressure.hold @03:24:25.97 SamplePump.in :prime @03:24:26.07 SamplePump.seek 150ml,7:39 @03:25:36.18 -> Can Can@03:25:00, 25.2C, 50% humidity, 14.1psia, 14.809V, 0.906A, 9.700Ah, 13.42W @03:27:28.70 Pressure.stop @03:27:28.81 Bypass.open @03:27:29.57 Intake.close @03:27:30.37 Toroid.in :hold @03:27:30.46 Toroid.seek :crack @03:27:32.04 Toroid.seek :cartridge @03:27:33.21 Toroid.in :default @03:27:33.31 Toroid.seek :clear @03:27:35.91 Pressurizing cartridge to 26psi above 14.6psia @03:27:35.93 SamplePump.setPosition! 0ml @03:27:36.02 Intake.open @03:27:36.69 Bypass.close @03:27:37.43 Exhaust.close @03:27:38.34 Pressure.in :default @03:27:38.43 Pressure.hold @03:27:38.70 SamplePump.in :charge @03:27:38.81 SamplePump.seek 2.3ml,42 seconds @03:27:48.24 Pressure.stop @03:27:48.34 Intake.close @03:27:49.10 Pressurized cartridge with 0.397ml to 26.4psi @03:27:49.12 Isolated @35.6psia (21.0psi) @03:27:49.18 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @03:27:49.26 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @03:27:53.87 Cartridge pressure fell 1% to 35.3psia @03:27:54.00 Sampling 1000.0ml @03:27:54.02 SamplePump.setPosition! 0ml @03:27:54.11 Exhaust.open @03:27:54.89 Intake.open @03:27:55.76 Pressure.in :default @03:27:55.86 Pressure.hold @03:27:56.02 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @03:27:56.26 SamplePump.in :sample @03:27:56.36 SamplePump.seek 1000ml,1:23:51 @03:27:59.38 Cartridge 11 filtering 1/1000ml at 0.56ml/s with 29.1psi vacuum @03:28:59.41 Cartridge 11 filtering 34/1000ml at 0.49ml/s with 19.4psi vacuum @03:29:59.39 Cartridge 11 filtering 65/1000ml at 0.55ml/s with 24.9psi vacuum @03:30:59.38 Cartridge 11 filtering 95/1000ml at 0.43ml/s with 22.5psi vacuum @03:31:59.39 Cartridge 11 filtering 123/1000ml at 0.47ml/s with 15.1psi vacuum @03:32:59.38 Cartridge 11 filtering 151/1000ml at 0.46ml/s with 27.8psi vacuum @03:33:59.39 Cartridge 11 filtering 179/1000ml at 0.46ml/s with 16.4psi vacuum @03:34:59.38 Cartridge 11 filtering 205/1000ml at 0.38ml/s with 26.3psi vacuum @03:35:59.39 Cartridge 11 filtering 231/1000ml at 0.39ml/s with 30.3psi vacuum @03:36:59.38 Cartridge 11 filtering 257/1000ml at 0.46ml/s with 19.0psi vacuum @03:37:53.08 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [10,8,6] => { Type: :microSPR_phyto, State: :dry}, [9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 11 => { Type: :archiveHiBiomass_bac, State: :filtering, TV: :open}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, [56,54] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @03:37:59.38 Cartridge 11 filtering 282/1000ml at 0.46ml/s with 28.5psi vacuum @03:38:59.92 Cartridge 11 filtering 307/1000ml at 0.45ml/s with 29.7psi vacuum @03:39:59.39 Cartridge 11 filtering 332/1000ml at 0.36ml/s with 21.6psi vacuum @03:40:59.39 Cartridge 11 filtering 356/1000ml at 0.42ml/s with 14.7psi vacuum 2.0m deep (17.7psia) @03:41:59.39 Cartridge 11 filtering 380/1000ml at 0.42ml/s with 15.7psi vacuum 2.4m deep (18.3psia) @03:42:59.38 Cartridge 11 filtering 403/1000ml at 0.33ml/s with 26.4psi vacuum @03:43:59.39 Cartridge 11 filtering 426/1000ml at 0.43ml/s with 23.1psi vacuum @03:44:59.38 Cartridge 11 filtering 448/1000ml at 0.42ml/s with 23.1psi vacuum @03:45:59.39 Cartridge 11 filtering 471/1000ml at 0.41ml/s with 18.3psi vacuum 2.2m deep (17.9psia) @03:46:38.57 Session Begun @03:46:59.39 Cartridge 11 filtering 492/1000ml at 0.31ml/s with 26.7psi vacuum @03:47:59.38 Cartridge 11 filtering 514/1000ml at 0.38ml/s with 15.0psi vacuum @03:48:59.39 Cartridge 11 filtering 535/1000ml at 0.38ml/s with 27.4psi vacuum @03:49:59.39 Cartridge 11 filtering 555/1000ml at 0.38ml/s with 26.9psi vacuum @03:50:59.39 Cartridge 11 filtering 576/1000ml at 0.33ml/s with 13.1psi vacuum @03:51:13.96 Session Ended @03:51:59.38 Cartridge 11 filtering 595/1000ml at 0.32ml/s with 25.8psi vacuum @03:52:59.38 Cartridge 11 filtering 615/1000ml at 0.28ml/s with 29.2psi vacuum @03:53:59.39 Cartridge 11 filtering 634/1000ml at 0.31ml/s with 23.6psi vacuum @03:54:59.39 Cartridge 11 filtering 653/1000ml at 0.35ml/s with 23.5psi vacuum @03:55:59.39 Cartridge 11 filtering 671/1000ml at 0.27ml/s with 19.3psi vacuum @03:56:59.38 Cartridge 11 filtering 689/1000ml at 0.32ml/s with 29.4psi vacuum @03:57:59.39 Cartridge 11 filtering 707/1000ml at 0.24ml/s with 24.0psi vacuum @03:58:59.38 Cartridge 11 filtering 724/1000ml at 0.32ml/s with 30.3psi vacuum @03:59:59.39 Cartridge 11 filtering 741/1000ml at 0.27ml/s with 20.7psi vacuum @04:00:59.38 Cartridge 11 filtering 758/1000ml at 0.26ml/s with 26.8psi vacuum @04:01:59.39 Cartridge 11 filtering 774/1000ml at 0.24ml/s with 19.8psi vacuum @04:02:59.39 Cartridge 11 filtering 790/1000ml at 0.29ml/s with 30.9psi vacuum @04:03:59.39 Cartridge 11 filtering 805/1000ml at 0.24ml/s with 23.6psi vacuum @04:04:59.39 Cartridge 11 filtering 820/1000ml at 0.22ml/s with 28.8psi vacuum @04:05:59.39 Cartridge 11 filtering 835/1000ml at 0.21ml/s with 21.8psi vacuum @04:06:14.98 -> Can Can@04:04:52, 25.9C, 50% humidity, 14.1psia, 14.818V, 0.815A, 10.247Ah, 12.08W @04:06:59.38 Cartridge 11 filtering 850/1000ml at 0.25ml/s with 17.0psi vacuum @04:07:59.38 Cartridge 11 filtering 864/1000ml at 0.25ml/s with 22.8psi vacuum @04:08:59.38 Cartridge 11 filtering 878/1000ml at 0.21ml/s with 28.2psi vacuum @04:09:59.39 Cartridge 11 filtering 891/1000ml at 0.20ml/s with 24.1psi vacuum @04:10:59.39 Cartridge 11 filtering 905/1000ml at 0.20ml/s with 23.4psi vacuum @04:11:59.39 Cartridge 11 filtering 918/1000ml at 0.20ml/s with 27.4psi vacuum @04:12:59.39 Cartridge 11 filtering 931/1000ml at 0.23ml/s with 32.5psi vacuum @04:13:59.39 Cartridge 11 filtering 944/1000ml at 0.21ml/s with 19.7psi vacuum @04:14:23.61 Flow averaged 0.34ml/s over 46:28 @04:14:23.68 Waiting up to 13 seconds for vacuum to reduce from 27.9 to 18.0psi @04:14:24.29 Relieved excess vacuum in .7 seconds @04:14:24.35 SamplePump.seek 1000ml,4:44 @04:14:59.39 Cartridge 11 filtering 957/1000ml at 0.21ml/s with 19.2psi vacuum @04:15:37.33 Flow averaged 0.22ml/s over 1:13 @04:15:37.40 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @04:15:38.01 Relieved excess vacuum in .7 seconds @04:15:38.06 SamplePump.seek 1000ml,3:25 @04:15:59.38 Cartridge 11 filtering 970/1000ml at 0.20ml/s with 24.8psi vacuum @04:16:17.11 Flow averaged 0.21ml/s over 40 seconds @04:16:17.23 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @04:16:17.84 Relieved excess vacuum in .7 seconds @04:16:17.90 SamplePump.seek 1000ml,2:44 @04:16:47.00 Flow averaged 0.21ml/s over 30 seconds @04:16:47.08 Waiting up to 11 seconds for vacuum to reduce from 27.0 to 18.0psi @04:16:47.68 Relieved excess vacuum in .7 seconds @04:16:47.72 SamplePump.seek 1000ml,2:13 @04:16:59.38 Cartridge 11 filtering 982/1000ml at 0.20ml/s with 30.0psi vacuum @04:17:06.83 Flow averaged 0.21ml/s over 20 seconds @04:17:06.90 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @04:17:07.52 Relieved excess vacuum in .7 seconds @04:17:07.56 SamplePump.seek 1000ml,1:53 @04:17:23.65 Flow averaged 0.21ml/s over 17 seconds @04:17:23.73 Waiting up to 16 seconds for vacuum to reduce from 29.0 to 18.0psi @04:17:24.35 Relieved excess vacuum in .7 seconds @04:17:24.40 SamplePump.seek 1000ml,1:36 @04:17:39.28 Flow averaged 0.21ml/s over 15 seconds @04:17:39.36 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @04:17:39.96 Relieved excess vacuum in .7 seconds @04:17:40.02 SamplePump.seek 1000ml,1:20 @04:17:54.69 Flow averaged 0.21ml/s over 15 seconds @04:17:54.77 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @04:17:55.38 Relieved excess vacuum in .7 seconds @04:17:55.43 SamplePump.seek 1000ml,1:05 @04:17:59.38 Cartridge 11 filtering 994/1000ml at 0.20ml/s with 24.9psi vacuum @04:18:07.27 Flow averaged 0.21ml/s over 12 seconds @04:18:07.35 Waiting up to 17 seconds for vacuum to reduce from 29.5 to 18.0psi @04:18:07.95 Relieved excess vacuum in .7 seconds @04:18:08.01 SamplePump.seek 1000ml,53 seconds @04:18:19.98 Flow averaged 0.21ml/s over 12 seconds @04:18:20.05 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @04:18:21.33 Relieved excess vacuum in 1.4 seconds @04:18:21.38 SamplePump.seek 1000ml,40 seconds @04:18:30.85 Pressure.stop @04:18:31.03 Intake.close @04:18:31.18 Exhaust.close @04:18:31.42 Toroid.in :hold @04:18:31.52 Toroid.seek :bypass @04:18:33.21 Toroid.in :default @04:18:33.31 Toroid.seek :clear @04:18:34.95 Sampled 1000.0ml @04:18:35.45 GoodNews.email "After filtering 1000.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 11" @04:18:36.06 Plunge.seek :reagent @04:18:36.91 Sample loop closed. Ready to Deliver Reagents @04:18:36.95 Archive.seek :sealed @04:18:38.11 Gate.power :sampler,:OFF @04:18:40.94 Archive.seek :evac @04:18:48.27 upsync @04:18:55.01 delay 1:00 @04:19:55.05 Archive.seek :clear @04:20:12.56 RNAlater soak for 10:00 @04:30:12.60 Archive.seek :tVent @04:30:15.08 delay 1 second @04:30:16.11 Archive.seek :evac @04:30:31.64 delay 10 seconds @04:30:41.68 Archive.seek :tVent @04:30:57.21 delay 1 second @04:30:58.24 Archive.seek :evac @04:31:13.77 delay 10 seconds @04:31:23.81 Sample preserved and evacuated @04:31:23.98 Selecting Cartridge 10 @04:31:24.27 Carousel.in :default @04:31:24.43 Carousel.home.seek 11 @04:31:24.83 Archive.in :unlocking @04:31:25.02 Archive.seek :clear @04:31:25.19 Air.in :unlocking @04:31:25.35 Air.seek :clear @04:31:29.72 Lysis1.in :unlocking @04:31:30.47 Lysis1.seek :clear @04:31:30.53 Plunge.in :unlocking @04:31:30.70 Plunge.seek :clear @04:31:32.14 Carousel.seek 10 @04:31:32.71 Lysis1.in :fast @04:31:32.81 Lysis1.seek :locked @04:31:35.46 Plunge.in :default @04:31:35.62 Plunge.seek :locked @04:31:35.75 Air.in :fast @04:31:35.84 Air.seek :locked @04:31:37.43 Carousel.reconfigure :locked @04:31:37.65 Powering cartridge bus @04:31:40.31 Gate.setIR 1750,5000,0,6 @04:31:40.44 Gate.setIR 0,5000,0,6 @04:31:40.54 PuckHeater.configure :default @04:31:40.63 Gate.power :heat,:ON,limit to 5000mA @04:31:41.40 Mounted lysis Cartridge board#Q05060 @04:31:41.46 Using default cartridge heat loss curve for HeatingAccelInCarousel @04:31:41.50 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 10" @04:31:41.93 Preparing to filter cartridge 10 @04:31:42.26 Gate.power :sampler,:ON @04:31:42.39 Priming sample loop w/150ml, bypass w/50ml @04:31:42.40 SamplePump.setPosition! 0ml @04:31:42.49 Bypass.open @04:31:44.09 Intake.open @04:31:44.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:31:47.58 Vacuum tare is 56% of full scale @04:31:47.81 Pressure.in :default @04:31:47.92 Pressure.hold @04:31:48.14 upsync @04:31:48.27 Waiting for external sampler to finish priming... @04:31:48.29 delayUntil "04:35:12.28" @04:35:12.33 SamplePump.in :prime @04:35:12.43 SamplePump.seek 50ml,2:53 @04:35:23.91 Session Ended @04:36:13.42 Pressure.stop @04:36:13.48 SamplePump.setPosition! 0ml @04:36:13.53 Exhaust.open @04:36:14.97 Bypass.close @04:36:16.56 Pressure.in :default @04:36:16.62 Pressure.hold @04:36:16.93 SamplePump.in :prime @04:36:17.00 SamplePump.seek 150ml,7:39 @04:39:20.18 Pressure.stop @04:39:20.26 Bypass.open @04:39:21.03 Intake.close @04:39:21.81 Toroid.in :hold @04:39:21.88 Toroid.seek :crack @04:39:23.42 Toroid.seek :cartridge @04:39:24.53 Toroid.in :default @04:39:24.60 Toroid.seek :clear @04:39:26.62 Pressurizing cartridge to 26psi above 15.0psia @04:39:26.64 SamplePump.setPosition! 0ml @04:39:26.68 Intake.open @04:39:27.32 Bypass.close @04:39:28.04 Exhaust.close @04:39:28.88 Pressure.in :default @04:39:28.95 Pressure.hold @04:39:29.22 SamplePump.in :charge @04:39:29.29 SamplePump.seek 2.3ml,42 seconds @04:39:41.88 Pressure.stop @04:39:41.94 Intake.close @04:39:42.69 Pressurized cartridge with 0.718ml to 26.0psi @04:39:42.71 Isolated @35.6psia (20.6psi) @04:39:42.72 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @04:39:42.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @04:39:47.34 Cartridge pressure fell 1% to 35.4psia @04:39:47.45 Sampling 50.0ml @04:39:47.46 SamplePump.setPosition! 0ml @04:39:47.52 Exhaust.open @04:39:48.25 Intake.open @04:39:49.08 Pressure.in :default @04:39:49.17 Pressure.hold @04:39:49.34 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @04:39:49.54 SamplePump.in :sample @04:39:49.61 SamplePump.seek 50ml,4:41 @04:39:52.66 Cartridge 10 filtering 1/50ml at 0.63ml/s with 28.8psi vacuum @04:40:52.68 Cartridge 10 filtering 30/50ml at 0.43ml/s with 25.7psi vacuum @04:41:37.68 Pressure.stop @04:41:37.83 Intake.close @04:41:37.89 Exhaust.close @04:41:38.14 Toroid.in :hold @04:41:38.21 Toroid.seek :bypass @04:41:39.85 Toroid.in :default @04:41:39.92 Toroid.seek :clear @04:41:41.53 Sampled 50.0ml @04:41:42.02 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 10" @04:41:43.67 SPR.command "read_sequences" @04:41:44.19 SPR.command "stats_on" @04:41:44.44 SPR.command "start 0 1" @04:41:44.67 Gate.power :sampler,:OFF @04:41:45.10 SPR.start /var/log/esp/spr23aug1604h.spr.bz2 @04:41:47.05 Handoff.select :waste @04:41:47.88 upsync @04:41:53.86 Syringe tare is 18% of full scale @04:41:53.89 PinPort.in :locking @04:41:53.95 PinPort.seek :locked @04:41:56.49 Archive.in :fast @04:41:56.55 Lysis2.in :fast @04:41:56.60 Lysis1.seek 1350uL @04:41:56.77 Archive.seek :full @04:41:57.48 Lysis2.seek 950uL @04:42:02.19 Plunge.seek :reagent @04:42:03.10 Evacuating puck with filter bubble point of 50psi @04:42:03.13 PinPort.seek :locked @04:42:03.24 Handoff.select :waste @04:42:09.86 Air syringe pumping up to 1 strokes to reach 8psi @04:42:09.87 Air stroke #1 of 1 @04:42:09.96 Air.in :pressurize @04:42:10.03 Air.seek :pressurized @04:42:15.77 0.6psi after pressurize @04:42:15.80 Air.in :compress @04:42:15.86 Air.seek :evac @04:42:18.92 0.9psi after compress @04:42:29.52 0.1psi after compress (stable) @04:42:29.54 Air.in :default @04:42:30.01 Air.hold @04:42:30.41 at 0.0psi Opened to waste @04:42:30.42 Handoff.select :blocked @04:42:30.56 Archive syringe pumping up to 8 strokes to reach 50psi @04:42:30.57 Archive stroke #1 of 8 @04:42:30.66 Archive.in :pressurize @04:42:30.73 Archive.seek :pressurized @04:42:43.27 4.5psi after pressurize @04:42:51.39 5.1psi after pressurize (stable) @04:42:51.41 Archive.in :compress @04:42:51.48 Archive.seek :empty @04:43:06.15 15.8psi after compress @04:43:18.24 19.3psi after compress (stable) @04:43:18.27 Archive.in :gulp @04:43:18.40 Archive.seek :topVent @04:43:24.66 16.0psi after gulp @04:44:38.26 1.1psi after gulp (stable) @04:44:38.28 Archive stroke #2 of 8 @04:44:38.37 Archive.in :pressurize @04:44:38.44 Archive.seek :pressurized @04:44:50.94 0.9psi after pressurize @04:44:56.54 0.9psi after pressurize (stable) @04:44:56.56 Archive.in :compress @04:44:56.63 Archive.seek :empty @04:45:11.28 11.3psi after compress @04:45:36.88 5.2psi after compress (stable) @04:45:36.91 Archive.in :gulp @04:45:36.97 Archive.seek :topVent @04:45:43.27 4.4psi after gulp @04:45:56.88 1.2psi after gulp (stable) @04:45:56.89 Archive stroke #3 of 8 @04:45:56.98 Archive.in :pressurize @04:45:57.05 Archive.seek :pressurized @04:46:09.60 3.3psi after pressurize @04:46:19.70 3.9psi after pressurize (stable) @04:46:19.73 Archive.in :compress @04:46:19.79 Archive.seek :empty @04:46:34.42 6.7psi after compress @04:46:54.07 2.8psi after compress (stable) @04:46:54.09 Archive.in :gulp @04:46:54.16 Archive.seek :topVent @04:47:00.42 1.5psi after gulp @04:47:06.01 1.4psi after gulp (stable) @04:47:06.03 Archive stroke #4 of 8 @04:47:06.12 Archive.in :pressurize @04:47:06.19 Archive.seek :pressurized @04:47:18.71 1.3psi after pressurize @04:47:29.31 3.5psi after pressurize (stable) @04:47:29.33 Archive.in :compress @04:47:29.39 Archive.seek :empty @04:47:44.02 6.0psi after compress @04:48:04.56 2.8psi after compress (stable) @04:48:04.59 Archive.in :gulp @04:48:04.65 Archive.seek :topVent @04:48:10.91 2.4psi after gulp @04:48:19.02 1.7psi after gulp (stable) @04:48:19.03 Archive stroke #5 of 8 @04:48:19.20 Archive.in :pressurize @04:48:19.27 Archive.seek :pressurized @04:48:31.78 1.7psi after pressurize @04:48:42.02 3.6psi after pressurize (stable) @04:48:42.04 Archive.in :compress @04:48:42.11 Archive.seek :empty @04:48:56.75 6.4psi after compress @04:49:20.35 1.8psi after compress (stable) @04:49:20.37 Archive.in :gulp @04:49:20.43 Archive.seek :topVent @04:49:26.69 1.4psi after gulp @04:49:32.29 1.2psi after gulp (stable) @04:49:32.31 Archive stroke #6 of 8 @04:49:32.40 Archive.in :pressurize @04:49:32.47 Archive.seek :pressurized @04:49:44.97 1.1psi after pressurize @04:49:55.59 3.5psi after pressurize (stable) @04:49:55.63 Archive.in :compress @04:49:55.71 Archive.seek :empty @04:50:10.34 6.2psi after compress @04:50:33.94 1.9psi after compress (stable) @04:50:33.96 Archive.in :gulp @04:50:34.03 Archive.seek :topVent @04:50:40.28 1.1psi after gulp @04:50:45.88 1.0psi after gulp (stable) @04:50:45.90 Archive stroke #7 of 8 @04:50:45.99 Archive.in :pressurize @04:50:46.06 Archive.seek :pressurized @04:50:58.56 0.9psi after pressurize @04:51:09.67 3.3psi after pressurize (stable) @04:51:09.69 Archive.in :compress @04:51:09.75 Archive.seek :empty @04:51:24.38 5.9psi after compress @04:51:46.98 1.9psi after compress (stable) @04:51:47.00 Archive.in :gulp @04:51:47.07 Archive.seek :topVent @04:51:53.33 1.6psi after gulp @04:52:02.42 0.9psi after gulp (stable) @04:52:02.44 Archive stroke #8 of 8 @04:52:02.53 Archive.in :pressurize @04:52:02.60 Archive.seek :pressurized @04:52:15.10 0.9psi after pressurize @04:52:26.70 3.4psi after pressurize (stable) @04:52:26.72 Archive.in :compress @04:52:26.78 Archive.seek :empty @04:52:40.59 Session Begun @04:52:41.41 6.1psi after compress @04:52:47.51 -> exit @04:52:47.57 Session Ended @04:52:56.41 Session Begun @04:53:04.52 1.9psi after compress (stable) @04:53:04.54 Archive.in :default @04:53:04.66 Archive.hold @04:53:04.90 Handoff.select :blocked @04:53:04.97 Underpressure in PROCESSING -- Reached only 1.7 of 50.0psi after pumping 13840uL @04:53:04.99 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @04:53:05.03 BadNews.email "Reached only 1.7 of 50.0psi after pumping 13840uL",Subject: "Underpressure in PROCESSING" @04:53:11.68 upsync @04:56:11.26 -> exit @04:56:11.28 Session Ended @05:02:17.84 Session Begun @05:03:14.84 Ended log spr23aug1604h.spr.bz2 @05:03:14.91 SPR.command "stats_off" @05:03:54.81 Session Begun @05:04:00.18 -> Handoff Handoff Valve::Manifold selects blocked @05:04:05.71 -> Air Air Syringe (AirS) at evac @05:04:10.75 -> ArS Archive Syringe (ArS) empty @05:04:18.79 -> Syringe.psi 0.769885315452356 @05:04:41.47 -> Air.full; Air.evac @05:04:41.53 Air.seek :full @05:04:56.03 Air.seek :evac Air Syringe (AirS) at evac @05:05:20.11 -> Syringe.psi 5.27828476828513 @05:05:34.03 -> ArS.full; ArS.evac @05:05:34.08 Archive.seek :full @05:05:49.66 Archive.seek :evac Archive Syringe (ArS) empty @05:06:12.28 -> Syringe.psi 7.90040782975625 @05:06:35.65 -> Syringe.psi 3.91367930105513 @05:06:59.43 -> PV Plunge Valve (PV) at reagentLG @05:07:23.98 -> Air.pumpTo 20 @05:07:24.04 Air syringe pumping up to 8 strokes to reach 20psi @05:07:24.06 Air stroke #1 of 8 @05:07:24.21 Air.in :compress @05:07:24.32 Air.seek :evac @05:07:24.53 2.4psi after compress @05:07:35.16 2.1psi after compress (stable) @05:07:35.20 Air.in :gulp @05:07:35.33 Air.seek :topVent @05:07:41.24 2.3psi after gulp @05:07:46.84 2.1psi after gulp (stable) @05:07:46.86 Air stroke #2 of 8 @05:07:47.02 Air.in :pressurize @05:07:47.13 Air.seek :pressurized @05:07:54.23 2.4psi after pressurize @05:07:54.25 Air.in :compress @05:07:54.39 Air.seek :evac @05:07:57.46 11.3psi after compress @05:08:04.02 Session Begun @05:08:16.57 10.8psi after compress (stable) @05:08:16.60 Air.in :gulp @05:08:16.77 Air.seek :topVent @05:08:21.87 -> Syringe.psi 8.06868877302453 @05:08:22.65 7.9psi after gulp @05:08:33.65 -> Syringe.psi 5.48328155372103 @05:08:40.29 -> Syringe.psi 3.89991086024227 @05:08:41.26 3.9psi after gulp (stable) @05:08:41.28 Air stroke #3 of 8 @05:08:41.51 Air.in :pressurize @05:08:41.64 Air.seek :pressurized @05:08:48.19 4.4psi after pressurize @05:08:48.23 Air.in :compress @05:08:48.38 Air.seek :evac @05:08:50.92 Air.hold @05:08:51.19 Air.in :default @05:08:51.32 Air.hold @05:08:51.46 UserAbort in preston -- Air Syringe (AirS) seek to evac Aborted! @05:08:57.23 -> Air Air Syringe (AirS) at 152uL @05:09:01.87 -> Air.evac @05:09:01.95 Air.seek :evac Air Syringe (AirS) at evac @05:09:27.24 -> Handoff.waste @05:09:27.27 Handoff.select :waste Handoff Valve::Manifold selects waste @05:09:33.62 -> Syringe.psi 1.33439138877957 @05:09:39.06 -> Syringe.psi 0.623021946781861 @05:09:40.74 -> Syringe.psi 0.667386922734408 @05:09:48.74 -> exit @05:09:48.78 Session Ended @05:10:04.50 -> MainThread.exit @05:10:04.64 Handoff.select :close # @05:10:04.94 Session Ended @05:10:05.01 Session Ended @05:42:38.83PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @05:42:40.43 >BREAK< ESP3 Gateway boot v1.43 for core bus @05:42:40.45 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @05:42:43.73 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @05:42:44.32 Sea pressure (Abs) tare is 6% of full scale @05:42:55.39 Polling Can every 2:00 @05:42:55.44 Waiting for espclients... @05:49:23.02PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @05:49:26.78 >BREAK< ESP3 Gateway boot v1.43 for core bus @05:49:26.81 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @05:49:30.38 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @05:49:30.99 Sea pressure (Abs) tare is 6% of full scale @05:49:42.56 Polling Can every 2:00 @05:49:42.62 Waiting for espclients... Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @05:55:49.91PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @05:55:53.58 >BREAK< ESP3 Gateway boot v1.43 for core bus @05:55:53.61 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @05:55:57.10 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @05:55:57.70 Sea pressure (Abs) tare is 6% of full scale @05:56:09.09 Polling Can every 2:00 @05:56:09.15 Waiting for espclients... @05:56:31.92 Session Begun @05:59:20.18 -> Can Can@05:58:09, 24.8C, 51% humidity, 14.2psia, 14.885V, 0.081A, 0.006Ah, 1.21W @06:01:21.70 -> startDoublet # @06:01:21.93 Gate.power :sampler,:OFF,limit to 3500mA @06:01:21.96 Gate.power :main,:ON,limit to 5000mA @06:01:22.00 Gate.power :analytic1,:ON,limit to 5000mA @06:01:22.04 Gate.power :analytic2,:ON,limit to 5000mA @06:01:22.24 Powering core bus @06:01:24.48 SPR Module 1, FW Rev. B, FPGA Rev. C @06:01:25.03 Archive.configure :default @06:01:25.17 Air.configure :default @06:01:25.26 Lysis1.configure :default @06:01:25.37 Lysis2.configure :default @06:01:25.49 Plunge.configure :default @06:01:25.61 SamplePump.configure :default @06:01:25.77 Toroid.configure :default @06:01:25.90 Carousel.configure :default @06:01:26.02 Pressure.configure :default @06:01:26.14 ExtraPump.configure :default @06:01:26.23 PinPort.configure :default @06:01:26.33 All elf controllers running firmware version 4.84 @06:01:26.38 Gate.power :motor,:ON,limit to 5000mA @06:01:26.64 Archive.home.jog 0 @06:01:27.58 PinPort.home.jog 0 @06:01:27.64 Toroid.home.jog 0 @06:01:27.70 Plunge.home.jog 0 @06:01:27.74 Lysis1.home.jog 0 @06:01:27.79 Lysis2.home.jog 0 @06:01:27.84 Air.home.jog 0 @06:01:27.88 Carousel.home.jog 0 @06:01:28.65 Archive.in :homing @06:01:28.98 Archive.jog 1388 @06:01:29.09 Plunge.in :homing @06:01:29.15 Lysis1.in :homing @06:01:29.19 PinPort.in :homing @06:01:29.26 Air.in :homing @06:01:29.31 Toroid.in :homing @06:01:29.37 Lysis2.in :homing @06:01:29.77 Lysis1.jog 1387 @06:01:29.82 Plunge.jog 1564 @06:01:29.88 PinPort.jog -107 @06:01:29.92 Air.jog 1365 @06:01:29.96 Lysis2.jog 1392 @06:01:30.02 Toroid.jog 606 @06:01:30.39 PinPort.setRawPosition! 0 @06:01:30.48 PinPort.seek :home @06:01:31.01 PinPort.in :default @06:01:31.24 Lysis1.setRawPosition! 50 @06:01:31.28 Archive.setRawPosition! 50 @06:01:31.44 Lysis1.seek :home @06:01:31.47 Archive.seek :home @06:01:31.69 Plunge.setRawPosition! 50 @06:01:31.76 Lysis2.setRawPosition! 50 @06:01:31.86 Air.setRawPosition! 50 @06:01:32.00 Plunge.seek :home @06:01:32.03 Lysis2.seek :home @06:01:32.15 Air.seek :home @06:01:32.42 Toroid.setRawPosition! 0 @06:01:32.46 Lysis1.in :default @06:01:32.52 Plunge.in :default @06:01:32.57 Archive.in :default @06:01:32.80 Toroid.seek :home @06:01:32.97 Lysis2.in :default @06:01:33.02 Air.in :default @06:01:33.09 Toroid.in :default @06:01:33.56 Toroid.seek :clear @06:01:35.20 Carousel.in :default @06:01:35.37 Carousel.home.seek 10 @06:01:35.72 Selecting Cartridge 9 @06:01:36.08 Carousel.seek 9 @06:01:36.65 Lysis1.in :fast @06:01:36.76 Lysis1.seek :locked @06:01:39.42 Plunge.seek :locked @06:01:39.60 Air.in :fast @06:01:39.70 Air.seek :locked @06:01:41.30 Carousel.reconfigure :locked @06:01:41.50 Powering cartridge bus @06:01:44.17 Gate.setIR 1750,5000,0,6 @06:01:44.30 Gate.setIR 0,5000,0,6 @06:01:44.41 Gate.power :cartridge,:OFF @06:01:44.98 Mounted archive Cartridge @06:01:45.10 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 9" @06:01:45.51 Preparing to filter cartridge 9 @06:01:45.81 Gate.power :sampler,:ON @06:01:45.98 Priming sample loop w/150ml, bypass w/50ml @06:01:46.01 SamplePump.setPosition! 0ml @06:01:46.11 Bypass.open @06:01:47.78 Intake.open @06:01:48.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:01:51.18 Vacuum tare is 56% of full scale @06:01:51.60 Pressure.in :default @06:01:51.83 Pressure.hold @06:01:52.14 Waiting for external sampler to finish priming... @06:01:52.17 delayUntil "06:05:15.83" @06:01:52.33 upsync @06:05:15.88 SamplePump.in :prime @06:05:15.99 SamplePump.seek 50ml,2:53 @06:06:17.02 Pressure.stop @06:06:17.13 SamplePump.setPosition! 0ml @06:06:17.20 Exhaust.open @06:06:18.71 Bypass.close @06:06:20.31 Pressure.in :default @06:06:20.41 Pressure.hold @06:06:20.75 SamplePump.in :prime @06:06:20.85 SamplePump.seek 150ml,7:39 @06:09:23.81 Pressure.stop @06:09:23.92 Bypass.open @06:09:24.68 Intake.close @06:09:25.51 Toroid.in :hold @06:09:25.60 Toroid.seek :crack @06:09:27.19 Toroid.seek :cartridge @06:09:28.34 Toroid.in :default @06:09:28.44 Toroid.seek :clear @06:09:30.49 Pressurizing cartridge to 26psi above 14.5psia @06:09:30.51 SamplePump.setPosition! 0ml @06:09:30.59 Intake.open @06:09:31.26 Bypass.close @06:09:31.99 Exhaust.close @06:09:32.86 Pressure.in :default @06:09:32.95 Pressure.hold @06:09:33.26 SamplePump.in :charge @06:09:33.38 SamplePump.seek 2.3ml,42 seconds @06:09:42.29 Pressure.stop @06:09:42.39 Intake.close @06:09:43.24 Pressurized cartridge with 0.352ml to 26.1psi @06:09:43.26 Isolated @35.6psia (21.1psi) @06:09:43.28 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @06:09:43.35 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:09:48.05 Sampling 1000.0ml @06:09:48.07 SamplePump.setPosition! 0ml @06:09:48.22 Exhaust.open @06:09:49.06 Intake.open @06:09:49.90 Pressure.in :default @06:09:50.00 Pressure.hold @06:09:50.17 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @06:09:50.39 SamplePump.in :sample @06:09:50.50 SamplePump.seek 1000ml,1:23:51 @06:09:53.53 Cartridge 9 filtering 1/1000ml at 0.53ml/s with 28.0psi vacuum @06:10:53.54 Cartridge 9 filtering 30/1000ml at 0.49ml/s with 25.9psi vacuum @06:11:53.53 Cartridge 9 filtering 57/1000ml at 0.46ml/s with 27.2psi vacuum @06:12:53.63 Cartridge 9 filtering 83/1000ml at 0.41ml/s with 27.1psi vacuum @06:13:53.53 Cartridge 9 filtering 106/1000ml at 0.43ml/s with 28.5psi vacuum @06:14:53.63 Cartridge 9 filtering 130/1000ml at 0.33ml/s with 21.3psi vacuum @06:15:23.45 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [8,6] => { Type: :microSPR_phyto, State: :dry}, 7 => { Type: :archiveHiBiomass_bac, State: :dry}, 9 => { Type: :archiveHiBiomass_bac, State: :filtering, TV: :open}, 10 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1604h.spr.bz2"}, [56,54,11] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @06:15:53.54 Cartridge 9 filtering 152/1000ml at 0.35ml/s with 27.1psi vacuum @06:16:53.53 Cartridge 9 filtering 173/1000ml at 0.30ml/s with 21.6psi vacuum @06:17:53.53 Cartridge 9 filtering 194/1000ml at 0.37ml/s with 16.6psi vacuum @06:18:53.53 Cartridge 9 filtering 213/1000ml at 0.26ml/s with 22.1psi vacuum @06:19:53.53 Cartridge 9 filtering 231/1000ml at 0.36ml/s with 19.9psi vacuum 2.0m deep (17.7psia) @06:20:53.53 Cartridge 9 filtering 248/1000ml at 0.26ml/s with 28.0psi vacuum @06:21:53.53 Cartridge 9 filtering 263/1000ml at 0.25ml/s with 27.8psi vacuum @06:22:53.54 Cartridge 9 filtering 278/1000ml at 0.27ml/s with 16.7psi vacuum @06:23:53.53 Cartridge 9 filtering 292/1000ml at 0.20ml/s with 22.4psi vacuum @06:24:25.70 Flow averaged 0.34ml/s over 14:36 @06:24:25.78 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @06:24:26.39 Relieved excess vacuum in .7 seconds @06:24:26.45 SamplePump.seek 1000ml,58:57 @06:24:53.52 Cartridge 9 filtering 305/1000ml at 0.21ml/s with 20.8psi vacuum @06:24:54.23 Flow averaged 0.21ml/s over 28 seconds @06:24:54.30 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @06:24:54.91 Relieved excess vacuum in .7 seconds @06:24:54.96 SamplePump.seek 1000ml,58:28 @06:25:06.75 Flow averaged 0.21ml/s over 12 seconds @06:25:06.82 Waiting up to 23 seconds for vacuum to reduce from 31.9 to 18.0psi @06:25:07.43 Relieved excess vacuum in .7 seconds @06:25:08.07 SamplePump.seek 1000ml,58:15 @06:25:18.28 Flow averaged 0.19ml/s over 11 seconds @06:25:18.31 Intake.close @06:25:18.43 Exhaust.close @06:25:18.74 Toroid.in :hold @06:25:18.84 Toroid.seek :bypass @06:25:20.52 Toroid.in :default @06:25:20.62 Toroid.seek :clear @06:25:22.27 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @06:25:22.30 Pressure.stop @06:25:22.59 Sampled 309.2ml @06:25:23.11 GoodNews.email "After filtering 309.2ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 9" @06:25:23.78 Plunge.seek :reagent @06:25:24.65 Sample loop closed. Ready to Deliver Reagents @06:25:24.68 Archive.seek :sealed @06:25:25.75 Gate.power :sampler,:OFF @06:25:28.69 Archive.seek :evac @06:25:28.82 upsync @06:25:42.76 delay 1:00 @06:26:42.80 Archive.seek :clear @06:27:00.32 RNAlater soak for 10:00 @06:37:00.36 Archive.seek :tVent @06:37:02.84 delay 1 second @06:37:03.87 Archive.seek :evac @06:37:19.40 delay 10 seconds @06:37:29.44 Archive.seek :tVent @06:37:45.06 delay 1 second @06:37:46.09 Archive.seek :evac @06:38:01.63 delay 10 seconds @06:38:11.68 Sample preserved and evacuated @06:38:11.85 Selecting Cartridge 8 @06:38:12.13 Carousel.in :default @06:38:12.30 Carousel.home.seek 9 @06:38:12.70 Archive.in :unlocking @06:38:12.90 Archive.seek :clear @06:38:13.06 Air.in :unlocking @06:38:13.27 Air.seek :clear @06:38:18.09 Lysis1.in :unlocking @06:38:18.29 Lysis1.seek :clear @06:38:18.33 Plunge.in :unlocking @06:38:18.50 Plunge.seek :clear @06:38:19.93 Carousel.seek 8 @06:38:20.51 Lysis1.in :fast @06:38:20.61 Lysis1.seek :locked @06:38:23.26 Plunge.in :default @06:38:23.37 Plunge.seek :locked @06:38:23.55 Air.in :fast @06:38:23.64 Air.seek :locked @06:38:25.26 Carousel.reconfigure :locked @06:38:25.45 Powering cartridge bus @06:38:28.10 Gate.setIR 1750,5000,0,6 @06:38:28.24 Gate.setIR 0,5000,0,6 @06:38:28.33 PuckHeater.configure :default @06:38:28.42 Gate.power :heat,:ON,limit to 5000mA @06:38:29.22 Mounted lysis Cartridge board#Q05061 @06:38:29.29 Using this cartridge's heat loss curve for HeatingAccelInCarousel @06:38:29.33 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 8" @06:38:29.73 Preparing to filter cartridge 8 @06:38:30.08 Gate.power :sampler,:ON @06:38:30.22 Priming sample loop w/150ml, bypass w/50ml @06:38:30.25 SamplePump.setPosition! 0ml @06:38:30.34 Bypass.open @06:38:31.92 Intake.open @06:38:32.72 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:38:35.34 Vacuum tare is 56% of full scale @06:38:35.36 upsync @06:38:35.59 Pressure.in :default @06:38:35.69 Pressure.hold @06:38:35.96 Waiting for external sampler to finish priming... @06:38:35.98 delayUntil "06:42:00.09" @06:42:00.15 SamplePump.in :prime @06:42:00.82 SamplePump.seek 50ml,2:53 @06:43:01.85 Pressure.stop @06:43:01.93 SamplePump.setPosition! 0ml @06:43:02.00 Exhaust.open @06:43:03.45 Bypass.close @06:43:05.11 Pressure.in :default @06:43:05.21 Pressure.hold @06:43:05.56 SamplePump.in :prime @06:43:05.66 SamplePump.seek 150ml,7:39 @06:46:08.69 Pressure.stop @06:46:08.86 Bypass.open @06:46:09.60 Intake.close @06:46:10.45 Toroid.in :hold @06:46:10.54 Toroid.seek :crack @06:46:12.10 Toroid.seek :cartridge @06:46:13.24 Toroid.in :default @06:46:13.33 Toroid.seek :clear @06:46:15.38 Pressurizing cartridge to 26psi above 14.6psia @06:46:15.41 SamplePump.setPosition! 0ml @06:46:15.49 Intake.open @06:46:16.20 Bypass.close @06:46:16.93 Exhaust.close @06:46:17.81 Pressure.in :default @06:46:17.90 Pressure.hold @06:46:18.16 SamplePump.in :charge @06:46:18.26 SamplePump.seek 2.3ml,42 seconds @06:46:31.32 Pressure.stop @06:46:31.42 Intake.close @06:46:32.19 Pressurized cartridge with 0.768ml to 26.2psi @06:46:32.21 Isolated @35.6psia (21.0psi) @06:46:32.25 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @06:46:32.31 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:46:36.91 Cartridge pressure fell 1% to 35.3psia @06:46:37.08 Sampling 50.0ml @06:46:37.10 SamplePump.setPosition! 0ml @06:46:37.19 Exhaust.open @06:46:37.96 Intake.open @06:46:38.78 Pressure.in :default @06:46:38.88 Pressure.hold @06:46:39.09 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @06:46:39.87 SamplePump.in :sample @06:46:39.98 SamplePump.seek 50ml,4:41 @06:46:42.99 Cartridge 8 filtering 1/50ml at 0.51ml/s with 22.2psi vacuum @06:47:42.99 Cartridge 8 filtering 31/50ml at 0.45ml/s with 26.4psi vacuum @06:48:24.45 Pressure.stop @06:48:24.63 Intake.close @06:48:24.70 Exhaust.close @06:48:24.96 Toroid.in :hold @06:48:25.09 Toroid.seek :bypass @06:48:26.76 Toroid.in :default @06:48:26.86 Toroid.seek :clear @06:48:28.49 Sampled 50.0ml @06:48:28.97 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 8" @06:48:30.72 SPR.command "read_sequences" @06:48:31.44 SPR.command "stats_on" @06:48:31.69 SPR.command "start 0 1" @06:48:31.80 Gate.power :sampler,:OFF @06:48:32.34 SPR.start /var/log/esp/spr23aug1606h.spr.bz2 @06:48:34.28 Handoff.select :waste @06:48:34.73 upsync @06:48:41.06 Syringe tare is 18% of full scale @06:48:41.09 PinPort.in :locking @06:48:41.21 PinPort.seek :locked @06:48:43.78 Archive.in :fast @06:48:44.29 Lysis2.in :fast @06:48:44.50 Lysis1.seek 1350uL @06:48:44.72 Lysis2.seek 950uL @06:48:44.74 Archive.seek :full @06:48:49.48 Plunge.seek :reagent @06:48:50.41 Evacuating puck with filter bubble point of 50psi @06:48:50.44 PinPort.seek :locked @06:48:50.57 Handoff.select :waste @06:48:57.18 Air syringe pumping up to 1 strokes to reach 8psi @06:48:57.19 Air stroke #1 of 1 @06:48:57.34 Air.in :pressurize @06:48:57.48 Air.seek :pressurized @06:49:03.25 0.7psi after pressurize @06:49:03.28 Air.in :compress @06:49:03.38 Air.seek :evac @06:49:06.43 2.1psi after compress @06:49:17.06 1.0psi after compress (stable) @06:49:17.09 Air.in :default @06:49:17.19 Air.hold @06:49:17.52 at 0.7psi Opened to waste @06:49:17.54 Handoff.select :blocked @06:49:17.72 Archive syringe pumping up to 8 strokes to reach 50psi @06:49:17.74 Archive stroke #1 of 8 @06:49:17.88 Archive.in :pressurize @06:49:17.98 Archive.seek :pressurized @06:49:30.50 1.6psi after pressurize @06:49:36.11 1.6psi after pressurize (stable) @06:49:36.14 Archive.in :compress @06:49:36.29 Archive.seek :empty @06:49:50.93 15.3psi after compress @06:50:01.64 16.5psi after compress (stable) @06:50:01.67 Archive.in :gulp @06:50:01.81 Archive.seek :topVent @06:50:08.10 16.8psi after gulp @06:50:13.70 16.7psi after gulp (stable) @06:50:13.72 Archive stroke #2 of 8 @06:50:13.87 Archive.in :pressurize @06:50:13.96 Archive.seek :pressurized @06:50:26.53 16.6psi after pressurize @06:50:32.13 16.6psi after pressurize (stable) @06:50:32.16 Archive.in :compress @06:50:32.27 Archive.seek :empty @06:50:46.93 30.3psi after compress @06:50:57.58 31.4psi after compress (stable) @06:50:57.62 Archive.in :gulp @06:50:57.74 Archive.seek :topVent @06:51:04.04 31.6psi after gulp @06:51:09.64 31.5psi after gulp (stable) @06:51:09.66 Archive stroke #3 of 8 @06:51:09.81 Archive.in :pressurize @06:51:09.95 Archive.seek :pressurized @06:51:22.48 31.3psi after pressurize @06:51:28.11 31.2psi after pressurize (stable) @06:51:28.14 Archive.in :compress @06:51:28.27 Archive.seek :empty @06:51:42.92 44.1psi after compress @06:51:53.54 45.3psi after compress (stable) @06:51:53.58 Archive.in :gulp @06:51:53.69 Archive.seek :topVent @06:52:00.00 45.5psi after gulp @06:52:05.62 45.4psi after gulp (stable) @06:52:05.64 Archive stroke #4 of 8 @06:52:05.82 Archive.in :pressurize @06:52:05.92 Archive.seek :pressurized @06:52:18.45 45.2psi after pressurize @06:52:24.05 45.1psi after pressurize (stable) @06:52:24.08 Archive.in :compress @06:52:24.18 Archive.seek :empty @06:52:34.20 Reached 50.2 of 50.0psi after pumping 6819uL (3.94 strokes) @06:52:34.22 Archive.in :default @06:52:34.38 Archive.hold @06:52:34.54 Handoff.select :waste @06:52:46.29 delay 1:00 @06:53:46.34 Handoff.select :blocked @06:54:09.10 Handoff.select :waste @06:54:09.25 0.6psi at evacEnd @06:54:09.27 Handoff.select :blocked @06:54:09.44 Archive.in :fast @06:54:09.55 Archive.seek :topVent @06:54:22.98 Stable @0.6psi after Handoff blocked @06:54:23.04 Archive syringe pumping up to 8 strokes to reach 31psi @06:54:23.06 Archive stroke #1 of 8 @06:54:23.21 Archive.in :pressurize @06:54:23.31 Archive.seek :pressurized @06:54:35.83 0.8psi after pressurize @06:54:35.86 Archive.in :compress @06:54:35.97 Archive.seek :empty @06:54:50.65 12.5psi after compress @06:55:01.82 13.4psi after compress (stable) @06:55:01.85 Archive.in :gulp @06:55:01.96 Archive.seek :topVent @06:55:08.32 13.5psi after gulp @06:55:13.96 13.5psi after gulp (stable) @06:55:13.99 Archive stroke #2 of 8 @06:55:14.13 Archive.in :pressurize @06:55:14.22 Archive.seek :pressurized @06:55:26.76 13.5psi after pressurize @06:55:26.79 Archive.in :compress @06:55:26.90 Archive.seek :empty @06:55:41.56 24.7psi after compress @06:55:52.16 25.9psi after compress (stable) @06:55:52.19 Archive.in :gulp @06:55:52.30 Archive.seek :topVent @06:55:58.59 26.0psi after gulp @06:56:04.19 26.1psi after gulp (stable) @06:56:04.21 Archive stroke #3 of 8 @06:56:04.35 Archive.in :pressurize @06:56:04.44 Archive.seek :pressurized @06:56:16.97 26.0psi after pressurize @06:56:17.01 Archive.in :compress @06:56:17.15 Archive.seek :empty @06:56:25.90 Reached 30.8 of 30.6psi after pumping 5061uL (2.93 strokes) @06:56:25.93 Archive.in :default @06:56:26.04 Archive.hold @06:56:26.14 Handoff.select :waste @06:56:40.38 delay 1:00 @06:57:40.40 Handoff.select :blocked @06:58:03.14 Handoff.select :waste @06:58:03.85 0.8psi at evacEnd @06:58:03.88 Air.seek :fullVent @06:58:05.35 Archive.in :fast @06:58:05.44 Archive.seek :topVent @06:58:12.12 Seawater evacuated, Pressure above/below filter released. @06:58:12.25 Lysis1.in :slow @06:58:12.35 Lysis1.seek 1050uL @06:58:42.38 delay 5 seconds @06:58:47.40 Handoff.select :blocked @06:58:47.68 Lysis1.seek 450uL @06:59:47.63 delay 3 seconds @06:59:50.74 Air.in mix with 1.40048 parts Lysis1 @06:59:51.47 Air.seek :evac @06:59:51.53 Lysis1.seek 300uL @07:00:06.69 Lysis1.seek :empty @07:00:36.82 Lysis buffer delivered @7.1psi @07:00:36.84 Polling Can every 2:00 @07:00:37.42 PuckHeater.configure :default #ambient 25.5C @07:00:37.50 PuckHeater.seek 80.4C @07:01:24.96 PuckHeater.hold 2:00,80.4C @07:03:24.81 Polling Can every 2:00 @07:03:25.38 PuckHeater.configure :default #ambient 25.6C @07:03:25.46 PuckHeater.hold 30 seconds,79.4C @07:03:55.61 Polling Can every 2:00 @07:03:56.16 PuckHeater.configure :default #ambient 25.6C @07:03:56.25 PuckHeater.hold 30 seconds,78.8C @07:04:26.40 Polling Can every 2:00 @07:04:26.96 PuckHeater.hold 10:00,78.3C @07:14:25.84 Lysis complete. Syringe pressure @11.1psi @07:14:25.87 Archive syringe pumping up to 5 strokes to reach 40psi @07:14:25.92 Archive stroke #1 of 5 @07:14:26.07 Archive.in :pressurize @07:14:26.16 Archive.seek :pressurized @07:14:38.70 10.9psi after pressurize @07:14:44.30 10.8psi after pressurize (stable) @07:14:44.33 Archive.in :compress @07:14:44.44 Archive.seek :empty @07:14:59.16 34.2psi after compress @07:15:09.76 34.7psi after compress (stable) @07:15:09.79 Archive.in :gulp @07:15:09.89 Archive.seek :topVent @07:15:16.18 34.6psi after gulp @07:15:21.83 34.4psi after gulp (stable) @07:15:22.37 Archive stroke #2 of 5 @07:15:22.51 Archive.in :pressurize @07:15:22.60 Archive.seek :pressurized @07:15:35.14 34.0psi after pressurize @07:15:40.75 33.8psi after pressurize (stable) @07:15:40.78 Archive.in :compress @07:15:40.89 Archive.seek :empty @07:15:48.01 Reached 40.2 of 40.0psi after pumping 3297uL (1.91 strokes) @07:15:48.04 Archive.in :default @07:15:48.14 Archive.hold @07:15:48.28 delay 2 seconds @07:15:50.32 Archive.in :fast @07:15:50.41 Archive.seek :full @07:15:57.09 Lysis1.seek 138uL @07:16:10.98 delay 1 second @07:16:12.03 Archive.seek :full @07:16:12.10 Lysis1.seek :empty @07:16:26.07 Handoff.select :blocked @07:16:26.76 Initial air pressure @42.9psi @07:16:26.86 Lysis1.in :extract @07:16:27.07 Lysis1.seek 600uL,nil @07:16:32.79 Initial 600uL lysate @19.6psi @07:16:32.81 Handoff.select :blocked @07:16:32.96 Initial air pressure @19.8psi @07:16:33.11 Lysis1.seek 825uL,nil @07:16:35.08 Archive syringe pumping up to 6 strokes to reach 17psi @07:16:35.09 Archive stroke #1 of 6 @07:16:35.24 Archive.in :pressurize @07:16:35.35 Archive.seek :pressurized @07:16:35.54 Archive.in :default @07:16:35.62 Archive.hold @07:16:36.12 Recovered 825uL lysate @15.3psi @07:16:36.15 Air.in :halfSpeed @07:16:36.26 Air.seek :fullVent @07:16:38.50 Puck Top Vented, bottom @15.3psi @07:16:38.52 Handoff.select :waste @07:16:38.69 delay 5 seconds @07:16:43.78 Handoff open to waste, bottom @1.1psi @07:16:43.81 Air.in :default @07:16:43.91 Air.seek :evac @07:16:45.36 Lysis2.in :DAmix @07:16:46.12 Lysis1.in :DAmix @07:16:46.67 Lysis2.seek 700uL @07:16:46.71 Lysis1.seek 575uL @07:17:21.85 delay 5 seconds @07:17:26.89 Handoff.select SPR @07:17:27.05 SPR.command "start 2 14" @07:17:27.99 SPR stabilized @07:17:28.19 delay 10 seconds @07:17:38.22 SPR.command "start 2 0" @07:17:41.36 Waiting up to 11:00 for SPR to enter state 1 @07:19:39.05 SPR.command "hold_on" @07:19:40.42 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:19:41.16 Lysis2.seek 400uL @07:19:41.21 Lysis1.seek 275uL @07:20:23.25 delay 15 seconds @07:20:38.28 SPR.command "hold_off" @07:20:41.08 Waiting up to 38:00 for SPR to enter state 7 @07:35:01.25 SPRsummary:-498RIU,4e+09RIU,110RIU,148RIU,none,none,none after 2946s @07:46:31.68 SPR analysis completed @07:46:40.12 Ended log spr23aug1606h.spr.bz2 @07:46:40.15 SPR.command "stats_off" @07:46:40.74 Archive.stop @07:46:40.93 Handoff.select :close @07:46:41.12 Gate.power :heat,:OFF @07:46:41.15 Gate.power :cartridge,:OFF @07:46:41.28 PinPort.in :unlocking @07:46:41.36 PinPort.seek :clear @07:46:43.31 Carousel.in :default @07:46:43.48 Carousel.home.seek 8 @07:46:43.85 Plunge.seek :locked @07:46:44.78 Archive.in :unlocking @07:46:44.97 Archive.seek :clear @07:46:45.06 Air.in :unlocking @07:46:45.29 Air.seek :clear @07:46:45.42 Lysis1.in :unlocking @07:46:45.61 Lysis1.seek :clear @07:46:45.73 Lysis2.in :unlocking @07:46:45.87 Lysis2.seek :clear @07:46:49.73 Gate.power :motor,:OFF @07:46:49.76 Gate.power :core,:OFF @07:46:49.80 Gate.power :analytic1,:OFF @07:46:49.83 Gate.power :analytic2,:OFF @07:46:49.85 Gate.power :main,:OFF @07:46:49.88 Safely stopped and ready to power off @07:50:15.13 -> startDoublet # @07:50:15.38 Gate.power :sampler,:OFF,limit to 3500mA @07:50:15.41 Gate.power :main,:ON,limit to 5000mA @07:50:15.45 Gate.power :analytic1,:ON,limit to 5000mA @07:50:15.48 Gate.power :analytic2,:ON,limit to 5000mA @07:50:15.58 Powering core bus @07:50:17.93 SPR Module 1, FW Rev. B, FPGA Rev. C @07:50:18.35 Archive.configure :default @07:50:18.48 Air.configure :default @07:50:18.57 Lysis1.configure :default @07:50:18.66 Lysis2.configure :default @07:50:18.79 Plunge.configure :default @07:50:18.91 SamplePump.configure :default @07:50:19.07 Toroid.configure :default @07:50:19.19 Carousel.configure :default @07:50:19.31 Pressure.configure :default @07:50:19.43 ExtraPump.configure :default @07:50:19.52 PinPort.configure :default @07:50:19.61 All elf controllers running firmware version 4.84 @07:50:19.65 Gate.power :motor,:ON,limit to 5000mA @07:50:19.91 Archive.home.jog 0 @07:50:21.24 Carousel.home.jog 0 @07:50:21.31 Toroid.home.jog 0 @07:50:21.37 Plunge.home.jog 0 @07:50:21.42 Lysis1.home.jog 0 @07:50:21.46 Air.home.jog 0 @07:50:21.50 PinPort.home.jog 0 @07:50:21.56 Lysis2.home.jog 0 @07:50:22.55 Archive.in :homing @07:50:22.82 Archive.jog 1393 @07:50:22.90 Toroid.in :homing @07:50:22.96 Lysis2.in :homing @07:50:23.04 Air.in :homing @07:50:23.12 PinPort.in :homing @07:50:23.17 Plunge.in :homing @07:50:23.21 Lysis1.in :homing @07:50:23.55 Lysis2.jog 1393 @07:50:23.63 Toroid.jog 604 @07:50:23.72 Air.jog 1368 @07:50:23.80 PinPort.jog -107 @07:50:23.84 Plunge.jog 1572 @07:50:23.97 Lysis1.jog 1383 @07:50:24.36 PinPort.setRawPosition! 0 @07:50:24.44 PinPort.seek :home @07:50:24.58 Archive.setRawPosition! 50 @07:50:24.60 PinPort.in :default @07:50:24.75 Archive.seek :home @07:50:25.09 Archive.in :default @07:50:25.12 Lysis2.setRawPosition! 50 @07:50:25.30 Lysis2.seek :home @07:50:25.45 Lysis1.setRawPosition! 50 @07:50:25.49 Plunge.setRawPosition! 50 @07:50:25.54 Air.setRawPosition! 50 @07:50:25.70 Lysis1.seek :home @07:50:25.76 Plunge.seek :home @07:50:25.84 Air.seek :home @07:50:26.05 Toroid.setRawPosition! 0 @07:50:26.10 Lysis2.in :default @07:50:26.24 Toroid.seek :home @07:50:26.42 Plunge.in :default @07:50:26.47 Lysis1.in :default @07:50:26.51 Toroid.in :default @07:50:26.58 Air.in :default @07:50:27.09 Toroid.seek :clear @07:50:28.74 Carousel.in :default @07:50:28.91 Carousel.home.seek 8 @07:50:29.21 Selecting Cartridge 7 @07:50:29.55 Carousel.seek 7 @07:50:30.12 Lysis1.in :fast @07:50:30.23 Lysis1.seek :locked @07:50:33.38 Plunge.seek :locked @07:50:33.58 Air.in :fast @07:50:33.67 Air.seek :locked @07:50:35.30 Carousel.reconfigure :locked @07:50:35.49 Powering cartridge bus @07:50:38.16 Gate.setIR 1750,5000,0,6 @07:50:38.29 Gate.setIR 0,5000,0,6 @07:50:38.38 Gate.power :cartridge,:OFF @07:50:38.95 Mounted archive Cartridge @07:50:39.08 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 7" @07:50:39.47 Preparing to filter cartridge 7 @07:50:39.80 Gate.power :sampler,:ON @07:50:39.94 Priming sample loop w/150ml, bypass w/50ml @07:50:39.98 SamplePump.setPosition! 0ml @07:50:40.06 Bypass.open @07:50:41.63 Intake.open @07:50:42.45 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:50:45.19 Vacuum tare is 56% of full scale @07:50:45.59 Pressure.in :default @07:50:45.71 Pressure.hold @07:50:46.02 Waiting for external sampler to finish priming... @07:50:46.05 upsync @07:50:46.09 delayUntil "07:54:09.83" @07:54:09.88 SamplePump.in :prime @07:54:09.99 SamplePump.seek 50ml,2:53 @07:55:11.06 Pressure.stop @07:55:11.15 SamplePump.setPosition! 0ml @07:55:11.22 Exhaust.open @07:55:12.70 Bypass.close @07:55:14.29 Pressure.in :default @07:55:14.38 Pressure.hold @07:55:15.29 SamplePump.in :prime @07:55:15.39 SamplePump.seek 150ml,7:39 @07:57:33.83 -> Can Can@07:55:47, 25.4C, 50% humidity, 14.4psia, 14.813V, 0.910A, 1.324Ah, 13.47W @07:58:18.19 Pressure.stop @07:58:18.31 Bypass.open @07:58:19.06 Intake.close @07:58:19.92 Toroid.in :hold @07:58:20.01 Toroid.seek :crack @07:58:21.61 Toroid.seek :cartridge @07:58:22.76 Toroid.in :default @07:58:22.86 Toroid.seek :clear @07:58:24.91 Pressurizing cartridge to 26psi above 14.2psia @07:58:24.93 SamplePump.setPosition! 0ml @07:58:25.05 Intake.open @07:58:25.73 Bypass.close @07:58:26.47 Exhaust.close @07:58:27.33 Pressure.in :default @07:58:27.43 Pressure.hold @07:58:27.69 SamplePump.in :charge @07:58:27.79 SamplePump.seek 2.3ml,42 seconds @07:58:37.21 Pressure.stop @07:58:37.30 Intake.close @07:58:38.09 Pressurized cartridge with 0.396ml to 26.1psi @07:58:38.11 Isolated @35.3psia (21.1psi) @07:58:38.14 Verifying cartridge holds at least 0.65..0.85 of 35.3psia @07:58:38.18 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:58:42.89 Sampling 1000.0ml @07:58:42.91 SamplePump.setPosition! 0ml @07:58:43.03 Exhaust.open @07:58:43.77 Intake.open @07:58:44.66 Pressure.in :default @07:58:44.75 Pressure.hold @07:58:44.92 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @07:58:45.17 SamplePump.in :sample @07:58:45.28 SamplePump.seek 1000ml,1:23:51 @07:58:48.84 Cartridge 7 filtering 1/1000ml at 0.57ml/s with 24.3psi vacuum @07:59:48.31 Cartridge 7 filtering 31/1000ml at 0.48ml/s with 27.3psi vacuum @08:00:48.31 Cartridge 7 filtering 58/1000ml at 0.38ml/s with 26.8psi vacuum @08:01:48.31 Cartridge 7 filtering 82/1000ml at 0.35ml/s with 24.6psi vacuum @08:02:48.31 Cartridge 7 filtering 106/1000ml at 0.34ml/s with 19.3psi vacuum @08:03:48.30 Cartridge 7 filtering 127/1000ml at 0.41ml/s with 24.4psi vacuum @08:04:48.31 Cartridge 7 filtering 148/1000ml at 0.33ml/s with 29.0psi vacuum @08:05:48.30 Cartridge 7 filtering 167/1000ml at 0.29ml/s with 21.6psi vacuum @08:06:48.32 Cartridge 7 filtering 184/1000ml at 0.26ml/s with 18.7psi vacuum @08:07:48.31 Cartridge 7 filtering 200/1000ml at 0.26ml/s with 29.9psi vacuum @08:08:48.31 Cartridge 7 filtering 214/1000ml at 0.20ml/s with 30.1psi vacuum @08:09:17.84 Flow averaged 0.35ml/s over 10:33 @08:09:17.92 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @08:09:18.52 Relieved excess vacuum in .7 seconds @08:09:18.58 SamplePump.seek 1000ml,1:05:26 @08:09:32.22 Flow averaged 0.21ml/s over 14 seconds @08:09:32.30 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @08:09:32.91 Relieved excess vacuum in .7 seconds @08:09:32.95 SamplePump.seek 1000ml,1:05:12 @08:09:44.34 Flow averaged 0.21ml/s over 12 seconds @08:09:44.42 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @08:09:45.07 Relieved excess vacuum in .7 seconds @08:09:45.11 SamplePump.seek 1000ml,1:05:00 @08:09:48.30 Cartridge 7 filtering 227/1000ml at 0.20ml/s with 26.1psi vacuum @08:09:51.61 Flow estimate reduced to 0.17ml/s @08:09:51.64 Intake.close @08:09:51.75 Exhaust.close @08:09:52.05 Toroid.in :hold @08:09:52.15 Toroid.seek :bypass @08:09:53.84 Toroid.in :default @08:09:53.94 Toroid.seek :clear @08:09:55.58 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @08:09:55.61 Pressure.stop @08:09:55.90 Sampled 227.5ml @08:09:56.38 GoodNews.email "After filtering 227.5ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 7" @08:09:57.59 Plunge.seek :reagent @08:09:58.48 Sample loop closed. Ready to Deliver Reagents @08:09:58.52 Archive.seek :sealed @08:09:59.06 Gate.power :sampler,:OFF @08:10:02.51 Archive.seek :evac @08:10:04.52 upsync @08:10:16.58 delay 1:00 @08:11:16.61 Archive.seek :clear @08:11:34.19 RNAlater soak for 10:00 @08:21:34.22 Archive.seek :tVent @08:21:36.70 delay 1 second @08:21:37.73 Archive.seek :evac @08:21:53.29 delay 10 seconds @08:22:03.32 Archive.seek :tVent @08:22:18.86 delay 1 second @08:22:19.89 Archive.seek :evac @08:22:35.49 delay 10 seconds @08:22:45.52 Sample preserved and evacuated @08:22:45.68 Selecting Cartridge 6 @08:22:45.96 Carousel.in :default @08:22:46.12 Carousel.home.seek 7 @08:22:46.51 Archive.in :unlocking @08:22:46.70 Archive.seek :clear @08:22:46.81 Air.in :unlocking @08:22:47.06 Air.seek :clear @08:22:51.39 Lysis1.in :unlocking @08:22:51.57 Lysis1.seek :clear @08:22:51.64 Plunge.in :unlocking @08:22:51.78 Plunge.seek :clear @08:22:53.22 Carousel.seek 6 @08:22:53.79 Lysis1.in :fast @08:22:53.89 Lysis1.seek :locked @08:22:56.56 Plunge.in :default @08:22:56.73 Plunge.seek :locked @08:22:56.86 Air.in :fast @08:22:56.95 Air.seek :locked @08:22:59.10 Carousel.reconfigure :locked @08:22:59.31 Powering cartridge bus @08:23:01.98 Gate.setIR 1750,5000,0,6 @08:23:02.11 Gate.setIR 0,5000,0,6 @08:23:02.20 PuckHeater.configure :default #ambient 25.6C @08:23:02.29 Gate.power :heat,:ON,limit to 5000mA @08:23:03.05 Mounted lysis Cartridge board#Q05049 @08:23:03.10 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:23:03.18 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 6" @08:23:03.57 Preparing to filter cartridge 6 @08:23:03.91 Gate.power :sampler,:ON @08:23:04.04 Priming sample loop w/150ml, bypass w/50ml @08:23:04.06 SamplePump.setPosition! 0ml @08:23:04.17 Bypass.open @08:23:05.75 Intake.open @08:23:06.59 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:23:09.18 Vacuum tare is 56% of full scale @08:23:09.39 Pressure.in :default @08:23:09.44 upsync @08:23:09.56 Pressure.hold @08:23:09.83 Waiting for external sampler to finish priming... @08:23:09.85 delayUntil "08:26:33.94" @08:25:43.58 -> Can Can@08:25:40, 25.9C, 49% humidity, 14.4psia, 14.859V, 0.417A, 1.635Ah, 6.20W @08:26:33.99 SamplePump.in :prime @08:26:34.09 SamplePump.seek 50ml,2:53 @08:27:35.15 Pressure.stop @08:27:35.23 SamplePump.setPosition! 0ml @08:27:35.32 Exhaust.open @08:27:36.79 Bypass.close @08:27:38.43 Pressure.in :default @08:27:38.53 Pressure.hold @08:27:39.45 SamplePump.in :prime @08:27:39.55 SamplePump.seek 150ml,7:39 @08:30:42.11 Pressure.stop @08:30:42.22 Bypass.open @08:30:43.02 Intake.close @08:30:43.88 Toroid.in :hold @08:30:43.98 Toroid.seek :crack @08:30:45.55 Toroid.seek :cartridge @08:30:46.69 Toroid.in :default @08:30:46.78 Toroid.seek :clear @08:30:48.84 Pressurizing cartridge to 26psi above 14.1psia @08:30:48.86 SamplePump.setPosition! 0ml @08:30:48.96 Intake.open @08:30:49.68 Bypass.close @08:30:50.40 Exhaust.close @08:30:51.26 Pressure.in :default @08:30:51.35 Pressure.hold @08:30:51.60 SamplePump.in :charge @08:30:51.71 SamplePump.seek 2.3ml,42 seconds @08:31:04.75 Pressure.stop @08:31:04.85 Intake.close @08:31:05.66 Pressurized cartridge with 0.765ml to 26.3psi @08:31:05.68 Isolated @34.9psia (20.8psi) @08:31:05.70 Verifying cartridge holds at least 0.65..0.85 of 34.9psia @08:31:05.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:31:10.46 Sampling 50.0ml @08:31:10.48 SamplePump.setPosition! 0ml @08:31:10.58 Exhaust.open @08:31:11.30 Intake.open @08:31:12.16 Pressure.in :default @08:31:12.26 Pressure.hold @08:31:12.42 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @08:31:12.63 SamplePump.in :sample @08:31:12.75 SamplePump.seek 50ml,4:41 @08:31:15.76 Cartridge 6 filtering 1/50ml at 0.56ml/s with 28.6psi vacuum @08:32:16.22 Cartridge 6 filtering 31/50ml at 0.46ml/s with 26.6psi vacuum @08:32:55.09 Pressure.stop @08:32:55.28 Intake.close @08:32:55.35 Exhaust.close @08:32:55.60 Toroid.in :hold @08:32:55.70 Toroid.seek :bypass @08:32:57.37 Toroid.in :default @08:32:57.46 Toroid.seek :clear @08:32:59.11 Sampled 50.0ml @08:32:59.61 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 6" @08:33:01.33 SPR.command "read_sequences" @08:33:01.95 SPR.command "stats_on" @08:33:02.14 SPR.command "start 0 1" @08:33:02.28 Gate.power :sampler,:OFF @08:33:02.84 SPR.start /var/log/esp/spr23aug1608h.spr.bz2 @08:33:05.30 Handoff.select :waste @08:33:12.04 Syringe tare is 18% of full scale @08:33:12.07 PinPort.in :locking @08:33:12.19 PinPort.seek :locked @08:33:14.77 Archive.in :fast @08:33:14.86 Lysis2.in :fast @08:33:14.95 Lysis1.seek 1350uL @08:33:15.22 Archive.seek :full @08:33:15.25 Lysis2.seek 950uL @08:33:20.03 Plunge.seek :reagent @08:33:20.98 Evacuating puck with filter bubble point of 50psi @08:33:21.04 PinPort.seek :locked @08:33:21.19 Handoff.select :waste @08:33:27.81 Air syringe pumping up to 1 strokes to reach 8psi @08:33:27.82 Air stroke #1 of 1 @08:33:27.99 Air.in :pressurize @08:33:28.11 Air.seek :pressurized @08:33:33.89 1.5psi after pressurize @08:33:33.91 Air.in :compress @08:33:34.03 Air.seek :evac @08:33:37.10 0.9psi after compress @08:33:47.71 0.9psi after compress (stable) @08:33:47.73 Air.in :default @08:33:48.29 Air.hold @08:33:48.69 at 1.2psi Opened to waste @08:33:48.71 Handoff.select :blocked @08:33:48.92 Archive syringe pumping up to 8 strokes to reach 50psi @08:33:48.94 Archive stroke #1 of 8 @08:33:49.10 Archive.in :pressurize @08:33:49.19 Archive.seek :pressurized @08:34:01.73 2.7psi after pressurize @08:34:07.33 2.7psi after pressurize (stable) @08:34:07.35 Archive.in :compress @08:34:07.47 Archive.seek :empty @08:34:22.14 13.9psi after compress @08:34:22.44 upsync @08:34:32.75 14.7psi after compress (stable) @08:34:32.78 Archive.in :gulp @08:34:32.89 Archive.seek :topVent @08:34:39.18 14.9psi after gulp @08:34:44.79 15.0psi after gulp (stable) @08:34:44.80 Archive stroke #2 of 8 @08:34:44.95 Archive.in :pressurize @08:34:45.07 Archive.seek :pressurized @08:34:57.60 15.0psi after pressurize @08:35:13.70 20.1psi after pressurize (stable) @08:35:13.73 Archive.in :compress @08:35:13.85 Archive.seek :empty @08:35:28.53 28.0psi after compress @08:35:39.13 29.2psi after compress (stable) @08:35:39.16 Archive.in :gulp @08:35:39.27 Archive.seek :topVent @08:35:45.53 29.3psi after gulp @08:35:51.13 29.3psi after gulp (stable) @08:35:51.15 Archive stroke #3 of 8 @08:35:51.30 Archive.in :pressurize @08:35:51.39 Archive.seek :pressurized @08:36:03.91 29.2psi after pressurize @08:36:17.04 31.1psi after pressurize (stable) @08:36:17.08 Archive.in :compress @08:36:17.19 Archive.seek :empty @08:36:31.86 40.8psi after compress @08:36:42.46 42.1psi after compress (stable) @08:36:42.49 Archive.in :gulp @08:36:42.59 Archive.seek :topVent @08:36:48.88 42.3psi after gulp @08:36:54.48 42.3psi after gulp (stable) @08:36:54.49 Archive stroke #4 of 8 @08:36:54.63 Archive.in :pressurize @08:36:54.73 Archive.seek :pressurized @08:37:07.25 42.2psi after pressurize @08:37:12.85 42.1psi after pressurize (stable) @08:37:12.88 Archive.in :compress @08:37:13.03 Archive.seek :empty @08:37:24.56 Reached 50.2 of 50.0psi after pumping 6850uL (3.96 strokes) @08:37:24.58 Archive.in :default @08:37:24.70 Archive.hold @08:37:24.82 Handoff.select :waste @08:37:41.56 delay 1:00 @08:38:41.58 Handoff.select :blocked @08:39:04.31 Handoff.select :waste @08:39:04.47 1.5psi at evacEnd @08:39:04.49 Handoff.select :blocked @08:39:04.65 Archive.in :fast @08:39:04.79 Archive.seek :topVent @08:39:18.25 Stable @1.6psi after Handoff blocked @08:39:18.30 Archive syringe pumping up to 8 strokes to reach 32psi @08:39:18.32 Archive stroke #1 of 8 @08:39:18.45 Archive.in :pressurize @08:39:18.54 Archive.seek :pressurized @08:39:31.10 2.7psi after pressurize @08:39:31.13 Archive.in :compress @08:39:31.27 Archive.seek :empty @08:39:45.92 11.4psi after compress @08:39:56.53 12.0psi after compress (stable) @08:39:56.56 Archive.in :gulp @08:39:56.76 Archive.seek :topVent @08:40:03.13 12.0psi after gulp @08:40:08.73 12.0psi after gulp (stable) @08:40:08.75 Archive stroke #2 of 8 @08:40:08.90 Archive.in :pressurize @08:40:09.02 Archive.seek :pressurized @08:40:21.59 12.0psi after pressurize @08:40:21.62 Archive.in :compress @08:40:21.74 Archive.seek :empty @08:40:36.39 21.7psi after compress @08:40:46.99 22.2psi after compress (stable) @08:40:47.05 Archive.in :gulp @08:40:47.16 Archive.seek :topVent @08:40:53.46 22.3psi after gulp @08:40:59.12 22.2psi after gulp (stable) @08:40:59.15 Archive stroke #3 of 8 @08:40:59.34 Archive.in :pressurize @08:40:59.43 Archive.seek :pressurized @08:41:11.95 22.2psi after pressurize @08:41:11.98 Archive.in :compress @08:41:12.10 Archive.seek :empty @08:41:26.73 Reached 31.6 of 31.6psi after pumping 5189uL (3.00 strokes) @08:41:26.76 Archive.in :default @08:41:26.87 Archive.hold @08:41:26.98 Handoff.select :waste @08:41:51.27 delay 1:00 @08:42:51.30 Handoff.select :blocked @08:43:14.03 Handoff.select :waste @08:43:14.19 0.7psi at evacEnd @08:43:14.21 Air.seek :fullVent @08:43:15.68 Archive.in :fast @08:43:15.78 Archive.seek :topVent @08:43:22.92 Seawater evacuated, Pressure above/below filter released. @08:43:23.11 Lysis1.in :slow @08:43:23.20 Lysis1.seek 1050uL @08:43:53.23 delay 5 seconds @08:43:58.26 Handoff.select :blocked @08:43:58.50 Lysis1.seek 450uL @08:44:58.43 delay 3 seconds @08:45:01.55 Air.in mix with 1.40048 parts Lysis1 @08:45:02.32 Air.seek :evac @08:45:02.41 Lysis1.seek 300uL @08:45:17.55 Lysis1.seek :empty @08:45:47.71 Lysis buffer delivered @3.9psi @08:45:47.73 Polling Can every 2:00 @08:45:48.30 PuckHeater.configure :default #ambient 26.3C @08:45:48.38 PuckHeater.seek 79.6C @08:46:34.21 PuckHeater.hold 2:00,79.6C @08:48:33.77 Polling Can every 2:00 @08:48:34.32 PuckHeater.configure :default #ambient 26.4C @08:48:34.40 PuckHeater.hold 30 seconds,78.5C @08:49:04.46 Polling Can every 2:00 @08:49:05.05 PuckHeater.hold 30 seconds,78C @08:49:35.12 Polling Can every 2:00 @08:49:35.67 PuckHeater.configure :default #ambient 26.4C @08:49:35.76 PuckHeater.hold 10:00,77.5C @08:59:32.60 Lysis complete. Syringe pressure @6.3psi @08:59:32.63 Archive syringe pumping up to 5 strokes to reach 40psi @08:59:32.71 Archive stroke #1 of 5 @08:59:32.90 Archive.in :pressurize @08:59:33.00 Archive.seek :pressurized @08:59:45.57 6.4psi after pressurize @08:59:55.67 8.1psi after pressurize (stable) @08:59:55.70 Archive.in :compress @08:59:55.80 Archive.seek :empty @09:00:10.45 15.1psi after compress @09:00:21.08 15.7psi after compress (stable) @09:00:21.11 Archive.in :gulp @09:00:21.22 Archive.seek :topVent @09:00:27.52 15.7psi after gulp @09:00:33.12 15.7psi after gulp (stable) @09:00:33.13 Archive stroke #2 of 5 @09:00:33.28 Archive.in :pressurize @09:00:33.37 Archive.seek :pressurized @09:00:45.90 15.7psi after pressurize @09:00:57.01 19.3psi after pressurize (stable) @09:00:57.06 Archive.in :compress @09:00:57.18 Archive.seek :empty @09:01:11.84 24.8psi after compress @09:01:22.44 25.3psi after compress (stable) @09:01:22.46 Archive.in :gulp @09:01:22.57 Archive.seek :topVent @09:01:28.86 25.4psi after gulp @09:01:34.48 25.4psi after gulp (stable) @09:01:34.50 Archive stroke #3 of 5 @09:01:34.70 Archive.in :pressurize @09:01:34.85 Archive.seek :pressurized @09:01:47.36 25.3psi after pressurize @09:01:59.99 27.7psi after pressurize (stable) @09:02:00.03 Archive.in :compress @09:02:00.14 Archive.seek :empty @09:02:14.78 34.2psi after compress @09:02:25.38 34.6psi after compress (stable) @09:02:25.41 Archive.in :gulp @09:02:25.55 Archive.seek :topVent @09:02:31.83 34.7psi after gulp @09:02:37.44 34.7psi after gulp (stable) @09:02:37.45 Archive stroke #4 of 5 @09:02:37.60 Archive.in :pressurize @09:02:37.68 Archive.seek :pressurized @09:02:50.22 34.6psi after pressurize @09:02:55.83 34.5psi after pressurize (stable) @09:02:55.86 Archive.in :compress @09:02:56.42 Archive.seek :empty @09:03:06.63 Reached 40.1 of 40.0psi after pumping 6822uL (3.94 strokes) @09:03:06.66 Archive.in :default @09:03:06.76 Archive.hold @09:03:06.87 delay 2 seconds @09:03:08.90 Archive.in :fast @09:03:09.06 Archive.seek :full @09:03:15.92 Lysis1.seek 138uL @09:03:29.83 delay 1 second @09:03:30.89 Archive.seek :full @09:03:30.92 Lysis1.seek :empty @09:03:44.93 Handoff.select :blocked @09:03:45.14 Initial air pressure @41.2psi @09:03:45.26 Lysis1.in :extract @09:03:45.36 Lysis1.seek 600uL,nil @09:03:51.34 Initial 600uL lysate @28.0psi @09:03:51.36 Handoff.select :blocked @09:03:51.51 Initial air pressure @28.1psi @09:03:51.66 Lysis1.seek 825uL,nil @09:03:54.13 Recovered 825uL lysate @24.7psi @09:03:54.15 Air.in :halfSpeed @09:03:54.27 Air.seek :fullVent @09:03:56.52 Puck Top Vented, bottom @26.4psi @09:03:56.54 Handoff.select :waste @09:03:56.70 delay 5 seconds @09:04:01.80 Handoff open to waste, bottom @0.8psi @09:04:01.83 Air.in :default @09:04:01.95 Air.seek :evac @09:04:03.42 Lysis2.in :DAmix @09:04:03.59 Lysis1.in :DAmix @09:04:04.26 Lysis2.seek 700uL @09:04:04.34 Lysis1.seek 575uL @09:04:39.43 delay 5 seconds @09:04:44.45 Handoff.select SPR @09:04:44.63 SPR.command "start 2 14" @09:04:45.92 SPR stabilized @09:04:46.12 delay 10 seconds @09:04:56.14 SPR.command "start 2 0" @09:04:59.29 Waiting up to 11:00 for SPR to enter state 1 @09:06:56.96 SPR.command "hold_on" @09:06:58.34 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:06:59.09 Lysis2.seek 400uL @09:06:59.15 Lysis1.seek 275uL @09:07:41.20 delay 15 seconds @09:07:56.22 SPR.command "hold_off" @09:07:58.99 Waiting up to 38:00 for SPR to enter state 7 @09:22:19.18 SPRsummary:-139RIU,2.67e+09RIU,59.6RIU,133RIU,none,none,none after 3123s @09:26:59.33 -> Can Can@09:25:27, 26.5C, 49% humidity, 14.5psia, 14.849V, 0.526A, 2.442Ah, 7.82W @09:33:49.62 SPR analysis completed @09:33:58.90 Ended log spr23aug1608h.spr.bz2 @09:33:58.92 SPR.command "stats_off" @09:33:59.70 Handoff.select :close @09:33:59.87 Gate.power :heat,:OFF @09:33:59.89 Gate.power :cartridge,:OFF @09:34:00.01 PinPort.in :unlocking @09:34:00.10 PinPort.seek :clear @09:34:02.04 Carousel.in :default @09:34:02.20 Carousel.home.seek 6 @09:34:02.58 Plunge.seek :locked @09:34:03.50 Archive.in :unlocking @09:34:03.69 Archive.seek :clear @09:34:03.81 Air.in :unlocking @09:34:03.99 Air.seek :clear @09:34:04.12 Lysis1.in :unlocking @09:34:04.30 Lysis1.seek :clear @09:34:04.36 Lysis2.in :unlocking @09:34:04.52 Lysis2.seek :clear @09:34:08.45 Gate.power :motor,:OFF @09:34:08.48 Gate.power :core,:OFF @09:34:08.52 Gate.power :analytic1,:OFF @09:34:08.54 Gate.power :analytic2,:OFF @09:34:08.58 Gate.power :main,:OFF @09:34:08.61 Safely stopped and ready to power off @09:37:00.12 Session Ended @09:40:12.09 Session Begun @09:40:31.85 -> exit @09:40:31.87 Session Ended @09:42:22.37 Session Begun @09:43:09.99 -> doubleRegen @09:43:10.04 Gate.power :sampler,:OFF,limit to 3500mA @09:43:10.06 Gate.power :main,:ON,limit to 5000mA @09:43:10.09 Gate.power :analytic1,:ON,limit to 5000mA @09:43:10.14 Gate.power :analytic2,:ON,limit to 5000mA @09:43:10.24 Powering core bus @09:43:12.62 SPR Module 1, FW Rev. B, FPGA Rev. C @09:43:13.07 Archive.configure :default @09:43:13.20 Air.configure :default @09:43:13.29 Lysis1.configure :default @09:43:13.38 Lysis2.configure :default @09:43:13.49 Plunge.configure :default @09:43:13.61 SamplePump.configure :default @09:43:13.73 Toroid.configure :default @09:43:13.85 Carousel.configure :default @09:43:13.97 Pressure.configure :default @09:43:14.08 ExtraPump.configure :default @09:43:14.17 PinPort.configure :default @09:43:14.27 All elf controllers running firmware version 4.84 @09:43:14.31 Gate.power :motor,:ON,limit to 5000mA @09:43:14.55 Archive.home.jog 0 @09:43:15.37 PinPort.home.jog 0 @09:43:15.42 Toroid.home.jog 0 @09:43:15.48 Lysis2.home.jog 0 @09:43:15.52 Air.home.jog 0 @09:43:15.56 Carousel.home.jog 0 @09:43:15.61 Plunge.home.jog 0 @09:43:15.66 Lysis1.home.jog 0 @09:43:16.48 Archive.in :homing @09:43:16.79 Archive.jog 1389 @09:43:16.87 Toroid.in :homing @09:43:16.92 Lysis2.in :homing @09:43:16.97 Air.in :homing @09:43:17.04 Plunge.in :homing @09:43:17.10 Lysis1.in :homing @09:43:17.17 PinPort.in :homing @09:43:17.46 Toroid.jog 602 @09:43:17.52 Lysis2.jog 1395 @09:43:17.67 PinPort.jog -108 @09:43:17.72 Lysis1.jog 1386 @09:43:17.77 Plunge.jog 1568 @09:43:17.86 Air.jog 1366 @09:43:18.20 PinPort.setRawPosition! 0 @09:43:18.29 PinPort.seek :home @09:43:18.41 PinPort.in :default @09:43:18.53 Archive.setRawPosition! 50 @09:43:18.60 Archive.seek :home @09:43:18.90 Archive.in :default @09:43:19.05 Lysis2.setRawPosition! 50 @09:43:19.13 Lysis2.seek :home @09:43:19.26 Lysis1.setRawPosition! 50 @09:43:19.31 Air.setRawPosition! 50 @09:43:19.44 Lysis1.seek :home @09:43:19.48 Air.seek :home @09:43:19.69 Plunge.setRawPosition! 50 @09:43:19.72 Lysis2.in :default @09:43:19.86 Plunge.seek :home @09:43:20.00 Toroid.setRawPosition! 0 @09:43:20.05 Lysis1.in :default @09:43:20.08 Air.in :default @09:43:20.77 Toroid.seek :home @09:43:20.97 Plunge.in :default @09:43:21.01 Toroid.in :default @09:43:21.45 Toroid.seek :clear @09:43:23.09 Carousel.in :default @09:43:23.25 Carousel.home.seek 6 @09:43:24.77 SPR.command "read_sequences" @09:43:24.85 SPR.command "stats_on" @09:43:24.93 delay 2 seconds @09:43:25.73 SPR.start /var/log/esp/spr23aug1609h.spr.bz2 @09:43:26.95 SPR.command "start 3 0" @09:43:27.61 delay 15:00 @09:55:21.33 Session Begun @09:55:23.81 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 6 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1608h.spr.bz2}, 7 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 227.5}, 8 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr23aug1606h.spr.bz2}, 9 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 309.2}, 10 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1604h.spr.bz2"}, [56,54,11] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @09:55:55.61 -> exit @09:55:55.63 Session Ended @09:56:49.34 Session Begun @09:56:54.55 -> Cmd.stop @09:56:54.81 Carousel.in :default @09:56:55.07 Carousel.home.seek 6 @09:56:55.47 Lysis1.in :fast @09:56:55.58 Lysis1.seek :locked @09:56:58.14 Plunge.seek :locked @09:56:59.54 Lysis1.in :unlocking @09:56:59.76 Lysis1.seek :clear @09:57:05.80 Ended log spr23aug1609h.spr.bz2 @09:57:05.83 SPR.command "stats_off" @09:57:06.83 Gate.power :motor,:OFF @09:57:06.86 Gate.power :core,:OFF @09:57:06.91 Gate.power :analytic1,:OFF @09:57:06.94 Gate.power :analytic2,:OFF @09:57:06.99 Gate.power :main,:OFF @09:57:07.07 Safely stopped and ready to power off :STOPPED @09:58:06.15 -> exit @09:58:06.18 Session Ended @09:58:27.63 SPR.command "start 2 14" @09:58:27.69 AnalyticModule::SPR::Error in ben -- not started @11:42:09.15PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @11:42:10.80 >BREAK< ESP3 Gateway boot v1.43 for core bus @11:42:10.83 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @11:42:14.24 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @11:42:14.87 Sea pressure (Abs) tare is 6% of full scale @11:42:26.57 Polling Can every 2:00 @11:42:26.61 Loading /home/esp/esp2/bin/processAll mission @11:42:26.84 Sea pressure (Abs) tare is 6% of full scale @11:42:26.85 Sampling default goal volumes @11:42:26.86 Cartridge group: dry @11:42:26.93 Gate.power :sampler,:OFF,limit to 3500mA @11:42:26.94 Gate.power :main,:ON,limit to 5000mA @11:42:26.95 Gate.power :analytic1,:ON,limit to 5000mA @11:42:26.97 Gate.power :analytic2,:ON,limit to 5000mA @11:42:27.13 Powering core bus @11:42:29.38 SPR Module 1, FW Rev. B, FPGA Rev. C @11:42:29.92
Archive.configure :default @11:42:30.02 Air.configure :default @11:42:30.08 Lysis1.configure :default @11:42:30.14 Lysis2.configure :default @11:42:30.23 Plunge.configure :default @11:42:30.32 SamplePump.configure :default @11:42:30.41 Toroid.configure :default @11:42:30.50 Carousel.configure :default @11:42:30.59 Pressure.configure :default @11:42:30.69 ExtraPump.configure :default @11:42:30.75 PinPort.configure :default @11:42:30.81 All elf controllers running firmware version 4.84 @11:42:30.84 Gate.power :motor,:ON,limit to 5000mA @11:42:31.07 Archive.home.jog 0 @11:42:31.90 Toroid.home.jog 0 @11:42:31.95 Lysis2.home.jog 0 @11:42:31.99 Air.home.jog 0 @11:42:32.01 PinPort.home.jog 0 @11:42:32.03 Carousel.home.jog 0 @11:42:32.05 Lysis1.home.jog 0 @11:42:32.08 Plunge.home.jog 0 @11:42:32.76 Archive.in :homing @11:42:33.01 Archive.jog 1386 @11:42:33.21 Toroid.in :homing @11:42:33.28 Plunge.in :homing @11:42:33.31 Lysis1.in :homing @11:42:33.79 PinPort.in :homing @11:42:33.92 Lysis2.in :homing @11:42:33.95 Air.in :homing @11:42:34.25 Plunge.jog 1574 @11:42:34.28 Lysis1.jog 1387 @11:42:34.31 Toroid.jog 601 @11:42:34.34 Lysis2.jog 1389 @11:42:34.37 PinPort.jog -109 @11:42:34.41 Air.jog 1367 @11:42:34.77 Archive.setRawPosition! 50 @11:42:34.83 Archive.seek :home @11:42:34.89 PinPort.setRawPosition! 0 @11:42:35.00 PinPort.seek :home @11:42:35.09 PinPort.in :default @11:42:35.19 Archive.in :default @11:42:35.61 Lysis1.setRawPosition! 50 @11:42:35.67 Lysis1.seek :home @11:42:35.77 Plunge.setRawPosition! 50 @11:42:35.84 Lysis2.setRawPosition! 50 @11:42:35.98 Plunge.seek :home @11:42:36.01 Lysis2.seek :home @11:42:36.17 Lysis1.in :default @11:42:36.20 Air.setRawPosition! 50 @11:42:36.34 Air.seek :home @11:42:36.44 Lysis2.in :default @11:42:36.46 Plunge.in :default @11:42:36.66 Air.in :default @11:42:36.69 Toroid.setRawPosition! 0 @11:42:36.81 Toroid.seek :home @11:42:36.91 Toroid.in :default @11:42:37.07
Toroid.seek :clear @11:42:38.69 Carousel.in :default @11:42:38.82 Carousel.home.seek 6 @11:42:39.09 Selecting Cartridge 5 @11:42:39.40 Carousel.seek 5 @11:42:39.95 Lysis1.in :fast @11:42:40.01 Lysis1.seek :locked @11:42:42.64 Plunge.seek :locked @11:42:42.81 Air.in :fast @11:42:42.88 Air.seek :locked @11:42:44.45
Carousel.reconfigure :locked @11:42:44.62 Powering cartridge bus @11:42:47.27 Gate.setIR 1750,5000,0,6 @11:42:47.39 Gate.setIR 0,5000,0,6 @11:42:47.47 Gate.power :cartridge,:OFF @11:42:48.02 Mounted archive Cartridge @11:42:48.35 Preparing to filter cartridge 5 @11:42:48.60 Gate.power :sampler,:ON @11:42:48.69 Priming sample loop w/150ml, bypass w/50ml @11:42:48.72 SamplePump.setPosition! 0ml @11:42:48.76 Bypass.open @11:42:50.68 Intake.open @11:42:51.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:42:54.02 Vacuum tare is 56% of full scale @11:42:54.18 Pressure.in :default @11:42:54.24 Pressure.hold @11:42:54.47 Waiting for external sampler to finish priming... @11:42:54.48 delayUntil "11:46:18.61" @11:46:18.67 SamplePump.in :prime @11:46:18.74 SamplePump.seek 50ml,2:53 @11:47:19.70 Pressure.stop @11:47:19.78 SamplePump.setPosition! 0ml @11:47:19.83 Exhaust.open @11:47:21.28 Bypass.close @11:47:22.87 Pressure.in :default @11:47:22.93 Pressure.hold @11:47:23.24 SamplePump.in :prime @11:47:23.31 SamplePump.seek 150ml,7:39 @11:50:25.30 Pressure.stop @11:50:25.38 Bypass.open @11:50:26.13 Intake.close @11:50:26.92 Toroid.in :hold @11:50:26.98 Toroid.seek :crack @11:50:28.54 Toroid.seek :cartridge @11:50:29.66 Toroid.in :default @11:50:29.76 Toroid.seek :clear @11:50:31.81 Pressurizing cartridge to 26psi above 14.7psia @11:50:31.83 SamplePump.setPosition! 0ml @11:50:31.87 Intake.open @11:50:32.56 Bypass.close @11:50:33.27 Exhaust.close @11:50:34.12 Pressure.in :default @11:50:34.18 Pressure.hold @11:50:34.42 SamplePump.in :charge @11:50:34.49 SamplePump.seek 2.3ml,42 seconds @11:50:43.89 Pressure.stop @11:50:43.95 Intake.close @11:50:44.74 Pressurized cartridge with 0.398ml to 26.2psi @11:50:44.75 Isolated @35.2psia (20.5psi) @11:50:44.76 Verifying cartridge holds at least 0.65..0.85 of 35.2psia @11:50:44.79 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:50:49.46 Sampling 1000.0ml @11:50:49.48 SamplePump.setPosition! 0ml @11:50:49.53 Exhaust.open @11:50:50.26 Intake.open @11:50:51.11 Pressure.in :default @11:50:51.18 Pressure.hold @11:50:51.32 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @11:50:51.51 SamplePump.in :sample @11:50:51.58 SamplePump.seek 1000ml,1:23:51 @11:50:54.63 Cartridge 5 filtering 1/1000ml at 0.54ml/s with 21.7psi vacuum @11:51:54.64 Cartridge 5 filtering 35/1000ml at 0.56ml/s with 24.8psi vacuum @11:52:54.64 Cartridge 5 filtering 68/1000ml at 0.55ml/s with 22.5psi vacuum @11:53:54.64 Cartridge 5 filtering 101/1000ml at 0.55ml/s with 26.9psi vacuum @11:54:54.64 Cartridge 5 filtering 134/1000ml at 0.55ml/s with 26.2psi vacuum @11:55:54.64 Cartridge 5 filtering 166/1000ml at 0.53ml/s with 21.5psi vacuum @11:56:54.65 Cartridge 5 filtering 198/1000ml at 0.50ml/s with 22.7psi vacuum @11:57:54.65 Cartridge 5 filtering 229/1000ml at 0.52ml/s with 27.3psi vacuum @11:58:54.64 Cartridge 5 filtering 261/1000ml at 0.54ml/s with 24.6psi vacuum @11:59:54.64 Cartridge 5 filtering 292/1000ml at 0.51ml/s with 20.7psi vacuum @12:00:54.64 Cartridge 5 filtering 323/1000ml at 0.53ml/s with 25.2psi vacuum @12:01:54.63 Cartridge 5 filtering 354/1000ml at 0.54ml/s with 24.0psi vacuum @12:02:54.63 Cartridge 5 filtering 385/1000ml at 0.54ml/s with 24.3psi vacuum @12:03:54.64 Cartridge 5 filtering 416/1000ml at 0.54ml/s with 25.9psi vacuum @12:04:54.65 Cartridge 5 filtering 447/1000ml at 0.54ml/s with 24.7psi vacuum @12:05:54.64 Cartridge 5 filtering 478/1000ml at 0.49ml/s with 27.9psi vacuum @12:06:54.64 Cartridge 5 filtering 509/1000ml at 0.48ml/s with 26.0psi vacuum @12:07:54.64 Cartridge 5 filtering 540/1000ml at 0.49ml/s with 28.6psi vacuum @12:08:54.64 Cartridge 5 filtering 570/1000ml at 0.54ml/s with 20.9psi vacuum @12:09:54.64 Cartridge 5 filtering 601/1000ml at 0.54ml/s with 22.8psi vacuum @12:10:55.16 Cartridge 5 filtering 631/1000ml at 0.51ml/s with 18.6psi vacuum @12:11:54.64 Cartridge 5 filtering 662/1000ml at 0.54ml/s with 25.5psi vacuum @12:12:54.65 Cartridge 5 filtering 692/1000ml at 0.54ml/s with 25.8psi vacuum @12:13:54.64 Cartridge 5 filtering 723/1000ml at 0.54ml/s with 25.8psi vacuum @12:14:54.63 Cartridge 5 filtering 753/1000ml at 0.48ml/s with 21.3psi vacuum @12:15:54.64 Cartridge 5 filtering 784/1000ml at 0.51ml/s with 28.0psi vacuum @12:16:54.65 Cartridge 5 filtering 814/1000ml at 0.51ml/s with 28.7psi vacuum @12:17:54.64 Cartridge 5 filtering 844/1000ml at 0.54ml/s with 23.8psi vacuum @12:18:54.65 Cartridge 5 filtering 875/1000ml at 0.52ml/s with 19.2psi vacuum @12:19:54.65 Cartridge 5 filtering 905/1000ml at 0.48ml/s with 17.7psi vacuum @12:20:54.65 Cartridge 5 filtering 936/1000ml at 0.53ml/s with 26.7psi vacuum @12:21:54.65 Cartridge 5 filtering 966/1000ml at 0.52ml/s with 20.7psi vacuum @12:22:54.65 Cartridge 5 filtering 996/1000ml at 0.49ml/s with 27.7psi vacuum @12:23:02.94 Pressure.stop @12:23:03.08 Intake.close @12:23:03.12 Exhaust.close @12:23:03.37 Toroid.in :hold @12:23:03.44 Toroid.seek :bypass @12:23:05.08 Toroid.in :default @12:23:05.14 Toroid.seek :clear @12:23:06.76 Sampled 1000.0ml @12:23:07.42 Plunge.seek :reagent @12:23:08.23 Sample loop closed. Ready to Deliver Reagents @12:23:08.25 Archive.seek :sealed @12:23:09.89 Gate.power :sampler,:OFF @12:23:12.18 Archive.seek :evac @12:23:26.21 delay 1:00 @12:24:26.25 Archive.seek :clear @12:24:43.76 RNAlater soak for 10:00 @12:34:43.79 Archive.seek :tVent @12:34:46.24 delay 1 second @12:34:47.27 Archive.seek :evac @12:35:02.78 delay 10 seconds @12:35:12.81 Archive.seek :tVent @12:35:28.32 delay 1 second @12:35:29.34 Archive.seek :evac @12:35:44.85 delay 10 seconds @12:35:54.88 Sample preserved and evacuated @12:35:55.15
Carousel.in :default @12:35:55.29 Carousel.home.seek 5 @12:35:56.15 Plunge.seek :locked @12:35:57.06 Archive.in :unlocking @12:35:57.24 Archive.seek :clear @12:35:57.29 Air.in :unlocking @12:35:57.49 Air.seek :clear @12:35:57.63 Lysis1.in :unlocking @12:35:57.79 Lysis1.seek :clear @12:36:01.90
Gate.power :motor,:OFF @12:36:01.92 Gate.power :core,:OFF @12:36:01.93 Gate.power :analytic1,:OFF @12:36:01.95 Gate.power :analytic2,:OFF @12:36:01.96 Gate.power :main,:OFF @12:36:01.97 Safely stopped and ready to power off @12:36:02.46 Gate.power :sampler,:OFF,limit to 3500mA @12:36:05.75 Gate.power :main,:ON,limit to 5000mA @12:36:05.78 Gate.power :analytic1,:ON,limit to 5000mA @12:36:05.85 Gate.power :analytic2,:ON,limit to 5000mA @12:36:05.92 Powering core bus @12:36:08.28 SPR Module 1, FW Rev. B, FPGA Rev. C @12:36:08.70
Archive.configure :default @12:36:08.79 Air.configure :default @12:36:08.86 Lysis1.configure :default @12:36:08.93 Lysis2.configure :default @12:36:09.02 Plunge.configure :default @12:36:09.11 SamplePump.configure :default @12:36:09.21 Toroid.configure :default @12:36:09.30 Carousel.configure :default @12:36:09.40 Pressure.configure :default @12:36:09.49 ExtraPump.configure :default @12:36:09.55 PinPort.configure :default @12:36:09.62 All elf controllers running firmware version 4.84 @12:36:09.64 Gate.power :motor,:ON,limit to 5000mA @12:36:09.91 Archive.home.jog 0 @12:36:10.72 PinPort.home.jog 0 @12:36:10.77 Toroid.home.jog 0 @12:36:10.80 Lysis2.home.jog 0 @12:36:10.83 Lysis1.home.jog 0 @12:36:10.88 Plunge.home.jog 0 @12:36:10.91 Air.home.jog 0 @12:36:10.94 Carousel.home.jog 0 @12:36:11.68 Archive.in :homing @12:36:12.14 Toroid.in :homing @12:36:12.21 Plunge.in :homing @12:36:12.24 Lysis1.in :homing @12:36:12.30 Archive.jog 1387 @12:36:12.33 Lysis2.in :homing @12:36:12.37 PinPort.in :homing @12:36:12.41 Air.in :homing @12:36:12.72 Plunge.jog 1570 @12:36:12.79 Toroid.jog 606 @12:36:12.82 Lysis1.jog 1388 @12:36:12.86 Lysis2.jog 1393 @12:36:12.89 PinPort.jog -107 @12:36:12.92 Air.jog 1361 @12:36:13.97 PinPort.setRawPosition! 0 @12:36:13.99 Archive.setRawPosition! 50 @12:36:14.08 PinPort.seek :home @12:36:14.12 Archive.seek :home @12:36:14.38 Plunge.setRawPosition! 50 @12:36:14.45 Lysis2.setRawPosition! 50 @12:36:14.47 Air.setRawPosition! 50 @12:36:14.49 Lysis1.setRawPosition! 50 @12:36:14.52 PinPort.in :default @12:36:14.66 Plunge.seek :home @12:36:14.78 Lysis2.seek :home @12:36:14.83 Lysis1.seek :home @12:36:14.86 Air.seek :home @12:36:15.22 Archive.in :default @12:36:15.24 Toroid.setRawPosition! 0 @12:36:15.38 Toroid.seek :home @12:36:15.52 Toroid.in :default @12:36:15.58 Plunge.in :default @12:36:15.62 Lysis1.in :default @12:36:15.66 Lysis2.in :default @12:36:15.71 Air.in :default @12:36:16.25
Toroid.seek :clear @12:36:17.86 Carousel.in :default @12:36:18.01 Carousel.home.seek 5 @12:36:18.28 Selecting Cartridge 4 @12:36:18.60 Carousel.seek 4 @12:36:19.14 Lysis1.in :fast @12:36:19.20 Lysis1.seek :locked @12:36:21.86 Plunge.seek :locked @12:36:22.03 Air.in :fast @12:36:22.09 Air.seek :locked @12:36:23.67
Carousel.reconfigure :locked @12:36:23.86 Powering cartridge bus @12:36:26.52 Gate.setIR 1750,5000,0,6 @12:36:26.64 Gate.setIR 0,5000,0,6 @12:36:26.71 PuckHeater.configure :default @12:36:26.77 Gate.power :heat,:ON,limit to 5000mA @12:36:27.52 Mounted lysis Cartridge board#Q05051 @12:36:27.55 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:36:27.90 Preparing to filter cartridge 4 @12:36:28.16 Gate.power :sampler,:ON @12:36:28.25 Priming sample loop w/150ml, bypass w/50ml @12:36:28.27 SamplePump.setPosition! 0ml @12:36:28.31 Bypass.open @12:36:29.83 Intake.open @12:36:30.59 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:36:33.18 Vacuum tare is 56% of full scale @12:36:33.35 Pressure.in :default @12:36:33.42 Pressure.hold @12:36:33.64 Waiting for external sampler to finish priming... @12:36:33.66 delayUntil "12:39:58.17" @12:39:58.22 SamplePump.in :prime @12:39:58.29 SamplePump.seek 50ml,2:53 @12:40:59.28 Pressure.stop @12:40:59.34 SamplePump.setPosition! 0ml @12:40:59.39 Exhaust.open @12:41:00.83 Bypass.close @12:41:02.39 Pressure.in :default @12:41:02.45 Pressure.hold @12:41:02.76 SamplePump.in :prime @12:41:02.83 SamplePump.seek 150ml,7:39 @12:44:05.36 Pressure.stop @12:44:05.44 Bypass.open @12:44:06.18 Intake.close @12:44:06.96 Toroid.in :hold @12:44:07.03 Toroid.seek :crack @12:44:08.57 Toroid.seek :cartridge @12:44:09.68 Toroid.in :default @12:44:09.78 Toroid.seek :clear @12:44:11.84 Pressurizing cartridge to 26psi above 15.1psia @12:44:11.86 SamplePump.setPosition! 0ml @12:44:11.91 Intake.open @12:44:12.56 Bypass.close @12:44:13.28 Exhaust.close @12:44:14.11 Pressure.in :default @12:44:14.18 Pressure.hold @12:44:14.42 SamplePump.in :charge @12:44:14.50 SamplePump.seek 2.3ml,42 seconds @12:44:28.02 Pressure.stop @12:44:28.08 Intake.close @12:44:28.86 Pressurized cartridge with 0.830ml to 26.2psi @12:44:28.87 Isolated @35.6psia (20.5psi) @12:44:28.88 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @12:44:28.91 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:44:33.58 Sampling 50.0ml @12:44:33.60 SamplePump.setPosition! 0ml @12:44:33.65 Exhaust.open @12:44:34.36 Intake.open @12:44:35.21 Pressure.in :default @12:44:35.28 Pressure.hold @12:44:35.43 Sea 1.7m deep (17.1psia) @12:44:35.45 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @12:44:35.63 SamplePump.in :sample @12:44:35.70 SamplePump.seek 50ml,4:41 @12:44:38.75 Cartridge 4 filtering 2/50ml at 0.56ml/s with 25.8psi vacuum @12:45:38.76 Cartridge 4 filtering 36/50ml at 0.57ml/s with 22.5psi vacuum @12:46:02.99 Pressure.stop @12:46:03.13 Intake.close @12:46:03.17 Exhaust.close @12:46:03.43 Toroid.in :hold @12:46:03.49 Toroid.seek :bypass @12:46:05.12 Toroid.in :default @12:46:05.19 Toroid.seek :clear @12:46:06.80 Sampled 50.0ml @12:46:08.50 SPR.command "read_sequences" @12:46:08.75 SPR.command "stats_on" @12:46:08.92 SPR.command "start 0 1" @12:46:09.62 SPR.start /var/log/esp/spr23aug1612h.spr.bz2 @12:46:09.94 Gate.power :sampler,:OFF @12:46:11.60 Handoff.select :waste @12:46:18.91 Syringe tare is 18% of full scale @12:46:18.96 PinPort.in :locking @12:46:19.03 PinPort.seek :locked @12:46:21.58 Archive.in :fast @12:46:21.69 Lysis2.in :fast @12:46:21.79 Lysis1.seek 1350uL @12:46:21.82 Archive.seek :full @12:46:21.97 Lysis2.seek 950uL @12:46:26.67 Plunge.seek :reagent @12:46:27.57 Evacuating puck with filter bubble point of 50psi @12:46:27.60 PinPort.seek :locked @12:46:27.68 Handoff.select :waste @12:46:34.29 Air syringe pumping up to 1 strokes to reach 8psi @12:46:34.30 Air stroke #1 of 1 @12:46:34.40 Air.in :pressurize @12:46:34.47 Air.seek :pressurized @12:46:40.22 0.5psi after pressurize @12:46:40.24 Air.in :compress @12:46:40.30 Air.seek :evac @12:46:43.37 0.6psi after compress @12:46:53.98 0.2psi after compress (stable) @12:46:54.00 Air.in :default @12:46:54.06 Air.hold @12:46:54.28 at 0.1psi Opened to waste @12:46:54.30 Handoff.select :blocked @12:46:54.43 Archive syringe pumping up to 8 strokes to reach 50psi @12:46:54.45 Archive stroke #1 of 8 @12:46:54.54 Archive.in :pressurize @12:46:54.62 Archive.seek :pressurized @12:47:07.15 8.3psi after pressurize @12:47:12.76 8.4psi after pressurize (stable) @12:47:12.78 Archive.in :compress @12:47:12.84 Archive.seek :empty @12:47:27.47 22.9psi after compress @12:47:38.07 24.1psi after compress (stable) @12:47:38.09 Archive.in :gulp @12:47:38.20 Archive.seek :topVent @12:47:44.47 24.8psi after gulp @12:47:50.06 25.0psi after gulp (stable) @12:47:50.08 Archive stroke #2 of 8 @12:47:50.17 Archive.in :pressurize @12:47:50.23 Archive.seek :pressurized @12:48:02.76 24.7psi after pressurize @12:48:08.39 24.8psi after pressurize (stable) @12:48:08.45 Archive.in :compress @12:48:08.54 Archive.seek :empty @12:48:23.17 39.4psi after compress @12:48:33.79 40.8psi after compress (stable) @12:48:33.82 Archive.in :gulp @12:48:33.88 Archive.seek :topVent @12:48:40.16 41.7psi after gulp @12:48:45.78 41.3psi after gulp (stable) @12:48:45.80 Archive stroke #3 of 8 @12:48:45.90 Archive.in :pressurize @12:48:45.96 Archive.seek :pressurized @12:48:58.46 40.7psi after pressurize @12:49:04.06 40.6psi after pressurize (stable) @12:49:04.09 Archive.in :compress @12:49:04.15 Archive.seek :empty @12:49:16.34 Reached 50.2 of 50.0psi after pumping 5137uL (2.97 strokes) @12:49:16.36 Archive.in :default @12:49:16.42 Archive.hold @12:49:16.53 Handoff.select :waste @12:49:28.76 delay 1:00 @12:50:28.78 Handoff.select :blocked @12:50:51.48 Handoff.select :waste @12:50:51.61 0.2psi at evacEnd @12:50:51.62 Handoff.select :blocked @12:50:51.76 Archive.in :fast @12:50:51.83 Archive.seek :topVent @12:51:05.38 Stable @0.9psi after Handoff blocked @12:51:05.40 Archive syringe pumping up to 8 strokes to reach 31psi @12:51:05.41 Archive stroke #1 of 8 @12:51:05.52 Archive.in :pressurize @12:51:05.59 Archive.seek :pressurized @12:51:18.09 1.1psi after pressurize @12:51:18.11 Archive.in :compress @12:51:18.17 Archive.seek :empty @12:51:32.84 14.4psi after compress @12:51:43.94 16.0psi after compress (stable) @12:51:43.97 Archive.in :gulp @12:51:44.03 Archive.seek :topVent @12:51:50.34 16.2psi after gulp @12:51:55.94 16.2psi after gulp (stable) @12:51:55.95 Archive stroke #2 of 8 @12:51:56.04 Archive.in :pressurize @12:51:56.11 Archive.seek :pressurized @12:52:08.61 16.1psi after pressurize @12:52:08.65 Archive.in :compress @12:52:08.76 Archive.seek :empty @12:52:23.39 28.2psi after compress @12:52:34.49 29.9psi after compress (stable) @12:52:34.51 Archive.in :gulp @12:52:34.58 Archive.seek :topVent @12:52:40.87 30.1psi after gulp @12:52:46.46 30.0psi after gulp (stable) @12:52:46.49 Archive stroke #3 of 8 @12:52:46.62 Archive.in :pressurize @12:52:46.70 Archive.seek :pressurized @12:52:59.20 29.7psi after pressurize @12:52:59.22 Archive.in :compress @12:52:59.29 Archive.seek :empty @12:53:05.35 Reached 31.1 of 30.9psi after pumping 5005uL (2.89 strokes) @12:53:05.38 Archive.in :default @12:53:05.48 Archive.hold @12:53:05.56 Handoff.select :waste @12:53:17.82 delay 1:00 @12:54:17.84 Handoff.select :blocked @12:54:40.56 Handoff.select :waste @12:54:40.68 0.2psi at evacEnd @12:54:40.70 Air.seek :fullVent @12:54:42.12 Archive.in :fast @12:54:42.19 Archive.seek :topVent @12:54:48.65 Seawater evacuated, Pressure above/below filter released. @12:54:48.75 Lysis1.in :slow @12:54:48.82 Lysis1.seek 1050uL @12:55:18.84 delay 5 seconds @12:55:23.86 Handoff.select :blocked @12:55:24.07 Lysis1.seek 450uL @12:56:24.00 delay 3 seconds @12:56:27.11 Air.in mix with 1.40048 parts Lysis1 @12:56:27.87 Air.seek :evac @12:56:27.91 Lysis1.seek 300uL @12:56:43.05 Lysis1.seek :empty @12:57:13.15 Lysis buffer delivered @18.0psi @12:57:13.16 Polling Can every 2:00 @12:57:13.71 PuckHeater.configure :default #ambient 25.4C @12:57:13.79 PuckHeater.seek 79.6C @12:58:05.94 PuckHeater.hold 2:00,79.6C @13:00:05.99 Polling Can every 2:00 @13:00:06.53 PuckHeater.configure :default #ambient 25.5C @13:00:06.59 PuckHeater.hold 30 seconds,78.6C @13:00:36.76 Polling Can every 2:00 @13:00:37.31 PuckHeater.configure :default #ambient 25.5C @13:00:37.37 PuckHeater.hold 30 seconds,78.1C @13:01:07.55 Polling Can every 2:00 @13:01:08.11 PuckHeater.hold 10:00,77.5C @13:11:07.93 Lysis complete. Syringe pressure @17.0psi @13:11:07.95 Archive syringe pumping up to 5 strokes to reach 40psi @13:11:07.97 Archive stroke #1 of 5 @13:11:08.06 Archive.in :pressurize @13:11:08.12 Archive.seek :pressurized @13:11:20.62 16.1psi after pressurize @13:11:26.22 16.3psi after pressurize (stable) @13:11:26.25 Archive.in :compress @13:11:26.31 Archive.seek :empty @13:11:37.13 Reached 40.5 of 40.0psi after pumping 1647uL (0.95 strokes) @13:11:37.15 Archive.in :default @13:11:37.21 Archive.hold @13:11:37.28 delay 2 seconds @13:11:39.31 Archive.in :fast @13:11:39.37 Archive.seek :full @13:11:46.33 Lysis1.seek 138uL @13:12:00.20 delay 1 second @13:12:01.24 Archive.seek :full @13:12:01.27 Lysis1.seek :empty @13:12:15.22 Handoff.select :blocked @13:12:15.34 Initial air pressure @43.9psi @13:12:15.43 Lysis1.in :extract @13:12:15.56 Lysis1.seek 600uL,nil @13:12:17.36 Archive syringe pumping up to 6 strokes to reach 25psi @13:12:17.38 Archive stroke #1 of 6 @13:12:17.51 Archive.in :pressurize @13:12:17.58 Archive.seek :pressurized @13:12:30.15 14.5psi after pressurize @13:13:29.80 Archive.in :default @13:13:29.87 Archive.hold @13:13:30.04 Initial 600uL lysate @15.0psi @13:13:30.06 Handoff.select :blocked @13:13:30.16 Initial air pressure @15.0psi @13:13:30.27 Lysis1.seek 825uL,nil @13:13:30.39 Archive syringe pumping up to 6 strokes to reach 17psi @13:13:30.40 Archive stroke #1 of 6 @13:13:30.58 Archive.in :compress @13:13:30.66 Archive.seek :empty @13:13:39.45 Archive.in :default @13:13:39.53 Archive.hold @13:13:39.84 Recovered 825uL lysate @17.0psi @13:13:39.86 Air.in :halfSpeed @13:13:39.93 Air.seek :fullVent @13:13:42.15 Puck Top Vented, bottom @17.1psi @13:13:42.16 Handoff.select :waste @13:13:42.29 delay 5 seconds @13:13:47.38 Handoff open to waste, bottom @0.9psi @13:13:47.40 Air.in :default @13:13:47.46 Air.seek :evac @13:13:48.89 Lysis2.in :DAmix @13:13:49.02 Lysis1.in :DAmix @13:13:49.65 Lysis2.seek 700uL @13:13:49.71 Lysis1.seek 575uL @13:14:24.81 delay 5 seconds @13:14:29.84 Handoff.select SPR @13:14:30.00 SPR.command "start 2 14" @13:14:31.26 SPR stabilized @13:14:31.43 delay 10 seconds @13:14:41.45 SPR.command "start 2 0" @13:14:44.63 Waiting up to 11:00 for SPR to enter state 1 @13:16:42.29 SPR.command "hold_on" @13:16:43.68 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @13:16:44.36 Lysis2.seek 400uL @13:16:44.41 Lysis1.seek 275uL @13:17:26.44 delay 15 seconds @13:17:41.46 SPR.command "hold_off" @13:17:44.34 Waiting up to 38:00 for SPR to enter state 7 @13:32:04.53 SPRsummary:-17.1RIU,-19.1RIU,84.4RIU,110RIU,high,114ng/L,216ng/L after 2910s @13:43:34.96 SPR analysis completed @13:43:42.49 Ended log spr23aug1612h.spr.bz2 @13:43:42.51 SPR.command "stats_off" @13:43:43.15 Handoff.select :close @13:43:43.31
Gate.power :heat,:OFF @13:43:43.33 Gate.power :cartridge,:OFF @13:43:43.41 PinPort.in :unlocking @13:43:43.47 PinPort.seek :clear @13:43:45.39 Carousel.in :default @13:43:45.52 Carousel.home.seek 4 @13:43:45.90 Plunge.seek :locked @13:43:46.81 Archive.in :unlocking @13:43:46.97 Archive.seek :clear @13:43:47.04 Air.in :unlocking @13:43:47.24 Air.seek :clear @13:43:47.33 Lysis1.in :unlocking @13:43:47.51 Lysis1.seek :clear @13:43:47.56 Lysis2.in :unlocking @13:43:47.70 Lysis2.seek :clear @13:43:51.66
Gate.power :motor,:OFF @13:43:51.67 Gate.power :core,:OFF @13:43:51.69 Gate.power :analytic1,:OFF @13:43:51.70 Gate.power :analytic2,:OFF @13:43:51.71 Gate.power :main,:OFF @13:43:51.75 Safely stopped and ready to power off @13:43:51.94 Cartridge::None in MAIN -- Not enough dry cartridges remain @14:22:06.75PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @14:22:08.36 >BREAK< ESP3 Gateway boot v1.43 for core bus @14:22:08.39 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @14:22:11.59 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @14:22:12.22 Sea pressure (Abs) tare is 6% of full scale @14:22:23.29 Polling Can every 2:00 @14:22:23.33 Loading /home/esp/esp2/bin/processAll mission @14:22:23.54 Sea pressure (Abs) tare is 6% of full scale @14:22:23.55 Sampling maximum of 100.0ml per cartridge @14:22:23.57 Cartridge group: dry @14:22:23.63 Gate.power :sampler,:OFF,limit to 3500mA @14:22:23.64 Gate.power :main,:ON,limit to 5000mA @14:22:23.65 Gate.power :analytic1,:ON,limit to 5000mA @14:22:23.67 Gate.power :analytic2,:ON,limit to 5000mA @14:22:23.85 Powering core bus @14:22:26.09 SPR Module 1, FW Rev. B, FPGA Rev. C @14:22:26.61
Archive.configure :default @14:22:26.70 Air.configure :default @14:22:26.76 Lysis1.configure :default @14:22:26.82 Lysis2.configure :default @14:22:26.91 Plunge.configure :default @14:22:27.00 SamplePump.configure :default @14:22:27.09 Toroid.configure :default @14:22:27.18 Carousel.configure :default @14:22:27.26 Pressure.configure :default @14:22:27.35 ExtraPump.configure :default @14:22:27.42 PinPort.configure :default @14:22:27.49 All elf controllers running firmware version 4.84 @14:22:27.51 Gate.power :motor,:ON,limit to 5000mA @14:22:27.85 Archive.home.jog 0 @14:22:28.11 Air.home.jog 0 @14:22:28.25 Lysis1.home.jog 0 @14:22:28.36 Lysis2.home.jog 0 @14:22:28.50 Plunge.home.jog 0 @14:22:28.62 Toroid.home.jog 0 @14:22:28.79 Carousel.home.jog 0 @14:22:28.91 PinPort.home.jog 0 @14:22:29.45 Archive.in :homing @14:22:29.59 Lysis1.in :homing @14:22:29.69 Air.in :homing @14:22:29.77 Lysis2.in :homing @14:22:30.44 Archive.jog 1387 @14:22:30.52 Lysis1.jog 1387 @14:22:30.57 Air.jog 1367 @14:22:30.61 Lysis2.jog 1391 @14:22:30.94 PinPort.in :homing @14:22:30.97 Toroid.in :homing @14:22:31.01 Plunge.in :homing @14:22:31.15 PinPort.jog -107 @14:22:31.21 Plunge.jog 1568 @14:22:31.23 Toroid.jog 604 @14:22:31.48 PinPort.setRawPosition! 0 @14:22:31.54 PinPort.seek :home @14:22:31.62 PinPort.in :default @14:22:31.81 Archive.setRawPosition! 50 @14:22:31.87 Archive.seek :home @14:22:31.98 Lysis2.setRawPosition! 50 @14:22:32.01 Lysis1.setRawPosition! 50 @14:22:32.03 Air.setRawPosition! 50 @14:22:32.12 Lysis2.seek :home @14:22:32.24 Lysis1.seek :home @14:22:32.29 Air.seek :home @14:22:32.49 Archive.in :default @14:22:32.51 Lysis2.in :default @14:22:32.68 Plunge.setRawPosition! 50 @14:22:32.73 Lysis1.in :default @14:22:32.77 Air.in :default @14:22:32.85 Plunge.seek :home @14:22:33.21 Plunge.in :default @14:22:33.40 Toroid.setRawPosition! 0 @14:22:33.46 Toroid.seek :home @14:22:33.54 Toroid.in :default @14:22:33.63
Toroid.seek :clear @14:22:35.24 Carousel.in :default @14:22:35.37 Carousel.home.seek 4 @14:22:35.63 Selecting Cartridge 3 @14:22:35.97 Carousel.seek 3 @14:22:36.52 Lysis1.in :fast @14:22:36.58 Lysis1.seek :locked @14:22:39.20 Plunge.seek :locked @14:22:39.36 Air.in :fast @14:22:39.42 Air.seek :locked @14:22:41.00
Carousel.reconfigure :locked @14:22:41.16 Powering cartridge bus @14:22:43.81 Gate.setIR 1750,5000,0,6 @14:22:43.93 Gate.setIR 0,5000,0,6 @14:22:44.00 Gate.power :cartridge,:OFF @14:22:44.55 Mounted archive Cartridge @14:22:44.89 Preparing to filter cartridge 3 @14:22:45.14 Gate.power :sampler,:ON @14:22:45.23 Priming sample loop w/150ml, bypass w/50ml @14:22:45.25 SamplePump.setPosition! 0ml @14:22:45.82 Bypass.open @14:22:47.34 Intake.open @14:22:48.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:22:50.70 Vacuum tare is 56% of full scale @14:22:50.85 Pressure.in :default @14:22:50.92 Pressure.hold @14:22:51.13 Waiting for external sampler to finish priming... @14:22:51.15 delayUntil "14:26:15.16" @14:26:15.21 SamplePump.in :prime @14:26:15.28 SamplePump.seek 50ml,2:53 @14:27:16.21 Pressure.stop @14:27:16.25 SamplePump.setPosition! 0ml @14:27:16.31 Exhaust.open @14:27:17.72 Bypass.close @14:27:19.33 Pressure.in :default @14:27:19.39 Pressure.hold @14:27:19.70 SamplePump.in :prime @14:27:19.80 SamplePump.seek 150ml,7:39 @14:30:21.73 Pressure.stop @14:30:21.82 Bypass.open @14:30:22.55 Intake.close @14:30:23.36 Toroid.in :hold @14:30:23.43 Toroid.seek :crack @14:30:24.97 Toroid.seek :cartridge @14:30:26.08 Toroid.in :default @14:30:26.15 Toroid.seek :clear @14:30:28.16 Pressurizing cartridge to 26psi above 14.8psia @14:30:28.18 SamplePump.setPosition! 0ml @14:30:28.22 Intake.open @14:30:28.89 Bypass.close @14:30:29.61 Exhaust.close @14:30:30.45 Pressure.in :default @14:30:30.51 Pressure.hold @14:30:30.74 SamplePump.in :charge @14:30:30.82 SamplePump.seek 2.3ml,42 seconds @14:30:39.62 Pressure.stop @14:30:39.68 Intake.close @14:30:40.85 Pressurized cartridge with 0.348ml to 26.5psi @14:30:40.96 Isolated @35.5psia (20.7psi) @14:30:40.97 Verifying cartridge holds at least 0.65..0.85 of 35.5psia @14:30:41.00 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:30:45.59 Cartridge pressure fell 1% to 35.3psia @14:30:45.68 Sampling 100.0ml @14:30:45.70 SamplePump.setPosition! 0ml @14:30:45.78 Exhaust.open @14:30:46.51 Intake.open @14:30:47.33 Pressure.in :default @14:30:47.40 Pressure.hold @14:30:47.53 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @14:30:47.73 SamplePump.in :sample @14:30:47.83 SamplePump.seek 100ml,8:51 @14:30:50.84 Cartridge 3 filtering 1/100ml at 0.55ml/s with 27.6psi vacuum @14:31:50.86 Cartridge 3 filtering 31/100ml at 0.50ml/s with 22.8psi vacuum @14:32:50.85 Cartridge 3 filtering 58/100ml at 0.39ml/s with 19.5psi vacuum @14:33:50.86 Cartridge 3 filtering 84/100ml at 0.40ml/s with 27.6psi vacuum @14:34:33.03 Pressure.stop @14:34:33.17 Intake.close @14:34:33.22 Exhaust.close @14:34:33.46 Toroid.in :hold @14:34:33.53 Toroid.seek :bypass @14:34:35.16 Toroid.in :default @14:34:35.23 Toroid.seek :clear @14:34:36.84 Sampled 100.0ml @14:34:37.49 Plunge.seek :reagent @14:34:38.29 Sample loop closed. Ready to Deliver Reagents @14:34:38.31 Archive.seek :sealed @14:34:39.97 Gate.power :sampler,:OFF @14:34:42.24 Archive.seek :evac @14:34:56.27 delay 1:00 @14:35:56.31 Archive.seek :clear @14:36:13.79 RNAlater soak for 10:00 @14:46:13.82 Archive.seek :tVent @14:46:16.27 delay 1 second @14:46:17.29 Archive.seek :evac @14:46:32.79 delay 10 seconds @14:46:42.83 Archive.seek :tVent @14:46:58.33 delay 1 second @14:46:59.84 Archive.seek :evac @14:47:15.35 delay 10 seconds @14:47:25.37 Sample preserved and evacuated @14:47:25.66
Carousel.in :default @14:47:25.81 Carousel.home.seek 3 @14:47:26.14 Plunge.seek :locked @14:47:27.03 Archive.in :unlocking @14:47:27.20 Archive.seek :clear @14:47:27.25 Air.in :unlocking @14:47:27.46 Air.seek :clear @14:47:27.57 Lysis1.in :unlocking @14:47:27.77 Lysis1.seek :clear @14:47:31.87
Gate.power :motor,:OFF @14:47:31.89 Gate.power :core,:OFF @14:47:31.90 Gate.power :analytic1,:OFF @14:47:31.91 Gate.power :analytic2,:OFF @14:47:31.92 Gate.power :main,:OFF @14:47:31.93 Safely stopped and ready to power off @14:47:32.38 Cartridge::None in MAIN -- Not enough dry cartridges remain @14:54:43.05PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @14:54:44.66 >BREAK< ESP3 Gateway boot v1.43 for core bus @14:54:44.68 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @14:54:48.30 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @14:54:48.90 Sea pressure (Abs) tare is 6% of full scale @14:55:00.33 Polling Can every 2:00 @14:55:00.39 Loading /home/esp/esp2/bin/processAll mission @14:55:00.60 Sea pressure (Abs) tare is 6% of full scale @14:55:00.61 Sampling default goal volumes @14:55:00.63 Cartridge group: dry @14:55:00.69 Gate.power :sampler,:OFF,limit to 3500mA @14:55:00.70 Gate.power :main,:ON,limit to 5000mA @14:55:00.72 Gate.power :analytic1,:ON,limit to 5000mA @14:55:00.73 Gate.power :analytic2,:ON,limit to 5000mA @14:55:00.89 Powering core bus @14:55:03.15 SPR Module 1, FW Rev. B, FPGA Rev. C @14:55:03.66
Archive.configure :default @14:55:03.78 Air.configure :default @14:55:03.84 Lysis1.configure :default @14:55:03.91 Lysis2.configure :default @14:55:04.00 Plunge.configure :default @14:55:04.09 SamplePump.configure :default @14:55:04.18 Toroid.configure :default @14:55:04.27 Carousel.configure :default @14:55:04.36 Pressure.configure :default @14:55:04.45 ExtraPump.configure :default @14:55:04.52 PinPort.configure :default @14:55:04.59 All elf controllers running firmware version 4.84 @14:55:04.61 Gate.power :motor,:ON,limit to 5000mA @14:55:04.83 Archive.home.jog 0 @14:55:05.57 PinPort.home.jog 0 @14:55:05.63 Toroid.home.jog 0 @14:55:05.66 Plunge.home.jog 0 @14:55:05.68 Lysis1.home.jog 0 @14:55:05.70 Air.home.jog 0 @14:55:05.76 Lysis2.home.jog 0 @14:55:05.78 Carousel.home.jog 0 @14:55:06.49 Archive.in :homing @14:55:06.81 Archive.jog 1388 @14:55:07.34 Toroid.in :homing @14:55:07.37 Lysis2.in :homing @14:55:07.41 Plunge.in :homing @14:55:07.45 Lysis1.in :homing @14:55:07.49 Air.in :homing @14:55:07.52 PinPort.in :homing @14:55:07.86 Toroid.jog 602 @14:55:08.01 PinPort.jog -108 @14:55:08.07 Plunge.jog 1569 @14:55:08.10 Lysis1.jog 1383 @14:55:08.14 Air.jog 1359 @14:55:08.18 Lysis2.jog 1391 @14:55:08.53 PinPort.setRawPosition! 0 @14:55:08.58 PinPort.seek :home @14:55:08.68 PinPort.in :default @14:55:08.78 Archive.setRawPosition! 50 @14:55:08.83 Archive.seek :home @14:55:09.11 Archive.in :default @14:55:09.50 Lysis1.setRawPosition! 50 @14:55:09.57 Lysis1.seek :home @14:55:09.72 Plunge.setRawPosition! 50 @14:55:09.87 Lysis2.setRawPosition! 50 @14:55:09.91 Air.setRawPosition! 50 @14:55:10.04 Plunge.seek :home @14:55:10.08 Lysis2.seek :home @14:55:10.12 Air.seek :home @14:55:10.34 Lysis1.in :default @14:55:10.37 Toroid.setRawPosition! 0 @14:55:10.47 Toroid.seek :home @14:55:10.66 Toroid.in :default @14:55:10.71 Plunge.in :default @14:55:10.74 Lysis2.in :default @14:55:10.78 Air.in :default @14:55:11.26
Toroid.seek :clear @14:55:12.87 Carousel.in :default @14:55:13.01 Carousel.home.seek 3 @14:55:13.28 Selecting Cartridge 2 @14:55:13.59 Carousel.seek 2 @14:55:14.14 Lysis1.in :fast @14:55:14.20 Lysis1.seek :locked @14:55:16.82 Plunge.seek :locked @14:55:16.99 Air.in :fast @14:55:17.05 Air.seek :locked @14:55:18.63
Carousel.reconfigure :locked @14:55:18.79 Powering cartridge bus @14:55:21.45 Gate.setIR 1750,5000,0,6 @14:55:21.57 Gate.setIR 0,5000,0,6 @14:55:21.64 PuckHeater.configure :default @14:55:21.70 Gate.power :heat,:ON,limit to 5000mA @14:55:22.46 Mounted lysis Cartridge board#Q05087 @14:55:22.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:55:23.32 Preparing to filter cartridge 2 @14:55:23.57 Gate.power :sampler,:ON @14:55:23.67 Priming sample loop w/150ml, bypass w/50ml @14:55:23.69 SamplePump.setPosition! 0ml @14:55:23.76 Bypass.open @14:55:25.29 Intake.open @14:55:26.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:55:28.61 Vacuum tare is 56% of full scale @14:55:28.78 Pressure.in :default @14:55:28.84 Pressure.hold @14:55:29.07 Waiting for external sampler to finish priming... @14:55:29.08 delayUntil "14:58:53.58" @14:58:53.63 SamplePump.in :prime @14:58:53.71 SamplePump.seek 50ml,2:53 @14:59:54.73 Pressure.stop @14:59:54.78 SamplePump.setPosition! 0ml @14:59:54.84 Exhaust.open @14:59:56.30 Bypass.close @14:59:57.92 Pressure.in :default @14:59:57.99 Pressure.hold @14:59:58.29 SamplePump.in :prime @14:59:58.36 SamplePump.seek 150ml,7:39 @15:03:00.43 Pressure.stop @15:03:00.50 Bypass.open @15:03:01.26 Intake.close @15:03:02.03 Toroid.in :hold @15:03:02.10 Toroid.seek :crack @15:03:03.64 Toroid.seek :cartridge @15:03:04.75 Toroid.in :default @15:03:04.82 Toroid.seek :clear @15:03:06.84 Pressurizing cartridge to 26psi above 14.8psia @15:03:06.86 SamplePump.setPosition! 0ml @15:03:06.90 Intake.open @15:03:07.56 Bypass.close @15:03:08.28 Exhaust.close @15:03:09.10 Pressure.in :default @15:03:09.16 Pressure.hold @15:03:09.40 SamplePump.in :charge @15:03:10.00 SamplePump.seek 2.3ml,42 seconds @15:03:23.83 Pressure.stop @15:03:23.89 Intake.close @15:03:24.68 Pressurized cartridge with 0.869ml to 26.1psi @15:03:24.69 Isolated @35.2psia (20.4psi) @15:03:24.70 Verifying cartridge holds at least 0.65..0.85 of 35.2psia @15:03:24.73 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:03:29.41 Sampling 50.0ml @15:03:29.43 SamplePump.setPosition! 0ml @15:03:29.48 Exhaust.open @15:03:30.19 Intake.open @15:03:31.06 Pressure.in :default @15:03:31.12 Pressure.hold @15:03:31.26 Sea 1.7m deep (17.2psia) @15:03:31.28 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @15:03:31.48 SamplePump.in :sample @15:03:31.55 SamplePump.seek 50ml,4:41 @15:03:34.61 Cartridge 2 filtering 2/50ml at 0.55ml/s with 28.6psi vacuum @15:04:34.61 Cartridge 2 filtering 30/50ml at 0.46ml/s with 24.4psi vacuum @15:05:21.29 Pressure.stop @15:05:21.43 Intake.close @15:05:21.51 Exhaust.close @15:05:21.70 Toroid.in :hold @15:05:21.80 Toroid.seek :bypass @15:05:23.43 Toroid.in :default @15:05:23.50 Toroid.seek :clear @15:05:25.12 Sampled 50.0ml @15:05:26.83 SPR.command "read_sequences" @15:05:27.28 SPR.command "stats_on" @15:05:27.44 SPR.command "start 0 1" @15:05:28.14 SPR.start /var/log/esp/spr23aug1615h.spr.bz2 @15:05:28.25 Gate.power :sampler,:OFF @15:05:30.64 Handoff.select :waste @15:05:37.35 Syringe tare is 18% of full scale @15:05:37.37 PinPort.in :locking @15:05:37.44 PinPort.seek :locked @15:05:39.97 Archive.in :fast @15:05:40.07 Lysis1.seek 1350uL @15:05:40.15 Lysis2.in :fast @15:05:40.30 Archive.seek :full @15:05:40.37 Lysis2.seek 950uL @15:05:45.09 Plunge.seek :reagent @15:05:46.00 Evacuating puck with filter bubble point of 50psi @15:05:46.02 PinPort.seek :locked @15:05:46.11 Handoff.select :waste @15:05:52.72 Air syringe pumping up to 1 strokes to reach 8psi @15:05:52.74 Air stroke #1 of 1 @15:05:52.83 Air.in :pressurize @15:05:52.89 Air.seek :pressurized @15:05:58.64 1.2psi after pressurize @15:05:58.66 Air.in :compress @15:05:58.72 Air.seek :evac @15:06:01.76 0.9psi after compress @15:06:12.36 0.1psi after compress (stable) @15:06:12.38 Air.in :default @15:06:12.44 Air.hold @15:06:12.66 at 0.0psi Opened to waste @15:06:12.68 Handoff.select :blocked @15:06:12.81 Archive syringe pumping up to 8 strokes to reach 50psi @15:06:12.83 Archive stroke #1 of 8 @15:06:12.92 Archive.in :pressurize @15:06:12.98 Archive.seek :pressurized @15:06:25.50 5.4psi after pressurize @15:06:32.62 6.2psi after pressurize (stable) @15:06:32.64 Archive.in :compress @15:06:32.71 Archive.seek :empty @15:06:47.35 20.0psi after compress @15:07:00.48 23.5psi after compress (stable) @15:07:00.52 Archive.in :gulp @15:07:00.59 Archive.seek :topVent @15:07:06.85 24.1psi after gulp @15:07:12.97 24.1psi after gulp (stable) @15:07:13.00 Archive stroke #2 of 8 @15:07:13.09 Archive.in :pressurize @15:07:13.16 Archive.seek :pressurized @15:07:25.67 23.3psi after pressurize @15:07:31.27 23.6psi after pressurize (stable) @15:07:31.29 Archive.in :compress @15:07:31.35 Archive.seek :empty @15:07:45.98 38.2psi after compress @15:07:58.59 41.0psi after compress (stable) @15:07:58.61 Archive.in :gulp @15:07:58.67 Archive.seek :topVent @15:08:04.93 41.6psi after gulp @15:08:10.54 41.4psi after gulp (stable) @15:08:10.55 Archive stroke #3 of 8 @15:08:10.66 Archive.in :pressurize @15:08:10.72 Archive.seek :pressurized @15:08:23.22 40.5psi after pressurize @15:08:28.82 40.9psi after pressurize (stable) @15:08:28.85 Archive.in :compress @15:08:28.91 Archive.seek :empty @15:08:39.49 Reached 50.1 of 50.0psi after pumping 5102uL (2.95 strokes) @15:08:39.51 Archive.in :default @15:08:39.57 Archive.hold @15:08:39.65 Handoff.select :waste @15:08:53.87 delay 1:00 @15:09:53.89 Handoff.select :blocked @15:10:16.59 Handoff.select :waste @15:10:16.71 0.9psi at evacEnd @15:10:16.73 Handoff.select :blocked @15:10:16.85 Archive.in :fast @15:10:16.92 Archive.seek :topVent @15:10:30.30 Stable @0.9psi after Handoff blocked @15:10:30.32 Archive syringe pumping up to 8 strokes to reach 31psi @15:10:30.33 Archive stroke #1 of 8 @15:10:30.54 Archive.in :pressurize @15:10:30.62 Archive.seek :pressurized @15:10:43.11 1.6psi after pressurize @15:10:43.14 Archive.in :compress @15:10:43.20 Archive.seek :empty @15:10:57.87 14.1psi after compress @15:11:08.50 15.0psi after compress (stable) @15:11:08.52 Archive.in :gulp @15:11:08.59 Archive.seek :topVent @15:11:14.84 15.2psi after gulp @15:11:20.44 15.2psi after gulp (stable) @15:11:20.46 Archive stroke #2 of 8 @15:11:20.58 Archive.in :pressurize @15:11:20.68 Archive.seek :pressurized @15:11:33.18 15.1psi after pressurize @15:11:33.20 Archive.in :compress @15:11:33.79 Archive.seek :empty @15:11:48.45 27.9psi after compress @15:11:59.05 28.9psi after compress (stable) @15:11:59.07 Archive.in :gulp @15:11:59.14 Archive.seek :topVent @15:12:05.41 29.2psi after gulp @15:12:11.41 29.1psi after gulp (stable) @15:12:11.51 Archive stroke #3 of 8 @15:12:11.63 Archive.in :pressurize @15:12:11.75 Archive.seek :pressurized @15:12:24.27 28.8psi after pressurize @15:12:24.29 Archive.in :compress @15:12:24.35 Archive.seek :empty @15:12:29.69 Reached 31.1 of 30.9psi after pumping 4988uL (2.88 strokes) @15:12:29.71 Archive.in :default @15:12:29.81 Archive.hold @15:12:29.89 Handoff.select :waste @15:12:46.15 delay 1:00 @15:13:46.18 Handoff.select :blocked @15:14:08.96 Handoff.select :waste @15:14:09.08 0.4psi at evacEnd @15:14:09.10 Air.seek :fullVent @15:14:10.52 Archive.in :fast @15:14:10.58 Archive.seek :topVent @15:14:16.99 Seawater evacuated, Pressure above/below filter released. @15:14:17.08 Lysis1.in :slow @15:14:17.15 Lysis1.seek 1050uL @15:14:47.15 delay 5 seconds @15:14:52.17 Handoff.select :blocked @15:14:52.89 Lysis1.seek 450uL @15:15:52.84 delay 3 seconds @15:15:55.94 Air.in mix with 1.40048 parts Lysis1 @15:15:56.58 Air.seek :evac @15:15:56.62 Lysis1.seek 300uL @15:16:11.79 Lysis1.seek :empty @15:16:41.88 Lysis buffer delivered @13.0psi @15:16:41.90 Polling Can every 2:00 @15:16:42.44 PuckHeater.configure :default #ambient 25.3C @15:16:42.49 PuckHeater.seek 79.6C @15:17:33.57 PuckHeater.hold 2:00,79.6C @15:19:33.63 Polling Can every 2:00 @15:19:34.17 PuckHeater.configure :default #ambient 25.4C @15:19:34.23 PuckHeater.hold 30 seconds,78.6C @15:20:04.48 Polling Can every 2:00 @15:20:05.03 PuckHeater.hold 30 seconds,78.1C @15:20:35.25 Polling Can every 2:00 @15:20:35.82 PuckHeater.hold 10:00,77.5C @15:30:35.97 Lysis complete. Syringe pressure @17.0psi @15:30:35.99 Archive syringe pumping up to 5 strokes to reach 40psi @15:30:36.00 Archive stroke #1 of 5 @15:30:36.09 Archive.in :pressurize @15:30:36.16 Archive.seek :pressurized @15:30:48.67 16.8psi after pressurize @15:30:54.28 16.7psi after pressurize (stable) @15:30:54.30 Archive.in :compress @15:30:54.40 Archive.seek :empty @15:31:04.64 Reached 40.5 of 40.0psi after pumping 1635uL (0.95 strokes) @15:31:04.66 Archive.in :default @15:31:04.72 Archive.hold @15:31:04.85 delay 2 seconds @15:31:06.88 Archive.in :fast @15:31:06.94 Archive.seek :full @15:31:13.82 Lysis1.seek 138uL @15:31:27.70 delay 1 second @15:31:28.73 Archive.seek :full @15:31:28.77 Lysis1.seek :empty @15:31:42.71 Handoff.select :blocked @15:31:42.82 Initial air pressure @43.1psi @15:31:42.89 Lysis1.in :extract @15:31:43.02 Lysis1.seek 600uL,nil @15:31:47.17 Archive syringe pumping up to 6 strokes to reach 25psi @15:31:47.18 Archive stroke #1 of 6 @15:31:47.35 Archive.in :pressurize @15:31:47.47 Archive.seek :pressurized @15:31:48.69 Archive.in :default @15:31:48.77 Archive.hold @15:31:49.09 Initial 600uL lysate @13.0psi @15:31:49.11 Handoff.select :blocked @15:31:49.23 Initial air pressure @13.0psi @15:31:49.34 Lysis1.seek 825uL,nil @15:31:49.46 Archive syringe pumping up to 6 strokes to reach 17psi @15:31:49.47 Archive stroke #1 of 6 @15:31:50.21 Archive.in :pressurize @15:31:50.32 Archive.seek :pressurized @15:32:01.65 13.0psi after pressurize @15:32:23.81 15.9psi after pressurize (stable) @15:32:23.83 Archive.in :compress @15:32:23.92 Archive.seek :empty @15:32:30.98 Reached 17.1 of 17.0psi after pumping 1564uL (0.90 strokes) @15:32:31.00 Archive.in :default @15:32:31.06 Archive.hold @15:32:33.95 Recovered 825uL lysate @18.6psi @15:32:33.97 Air.in :halfSpeed @15:32:34.03 Air.seek :fullVent @15:32:36.26 Puck Top Vented, bottom @12.7psi @15:32:36.27 Handoff.select :waste @15:32:36.39 delay 5 seconds @15:32:41.48 Handoff open to waste, bottom @0.4psi @15:32:41.50 Air.in :default @15:32:41.56 Air.seek :evac @15:32:42.99 Lysis2.in :DAmix @15:32:43.15 Lysis1.in :DAmix @15:32:43.84 Lysis2.seek 700uL @15:32:43.87 Lysis1.seek 575uL @15:33:18.97 delay 5 seconds @15:33:23.99 Handoff.select SPR @15:33:24.12 SPR.command "start 2 14" @15:33:25.20 SPR stabilized @15:33:25.37 delay 10 seconds @15:33:35.39 SPR.command "start 2 0" @15:33:38.57 Waiting up to 11:00 for SPR to enter state 1 @15:35:36.24 SPR.command "hold_on" @15:35:37.63 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:35:38.33 Lysis2.seek 400uL @15:35:38.36 Lysis1.seek 275uL @15:36:20.38 delay 15 seconds @15:36:35.40 SPR.command "hold_off" @15:36:38.28 Waiting up to 38:00 for SPR to enter state 7 @15:50:58.47 SPRsummary:-17.2RIU,8.18RIU,62RIU,67.2RIU,high,193ng/L,388ng/L after 2884s @16:02:28.90 SPR analysis completed @16:02:36.02 Ended log spr23aug1615h.spr.bz2 @16:02:36.04 SPR.command "stats_off" @16:02:36.35 Handoff.select :close @16:02:36.50
Gate.power :heat,:OFF @16:02:36.52 Gate.power :cartridge,:OFF @16:02:36.60 PinPort.in :unlocking @16:02:36.67 PinPort.seek :clear @16:02:38.59 Carousel.in :default @16:02:38.73 Carousel.home.seek 2 @16:02:39.06 Plunge.seek :locked @16:02:39.96 Archive.in :unlocking @16:02:40.11 Archive.seek :clear @16:02:40.23 Air.in :unlocking @16:02:40.35 Air.seek :clear @16:02:40.47 Lysis1.in :unlocking @16:02:40.63 Lysis1.seek :clear @16:02:40.71 Lysis2.in :unlocking @16:02:40.82 Lysis2.seek :clear @16:02:44.75
Gate.power :motor,:OFF @16:02:44.76 Gate.power :core,:OFF @16:02:44.77 Gate.power :analytic1,:OFF @16:02:44.79 Gate.power :analytic2,:OFF @16:02:44.80 Gate.power :main,:OFF @16:02:44.81 Safely stopped and ready to power off @16:02:45.29 Gate.power :sampler,:OFF,limit to 3500mA @16:02:48.58 Gate.power :main,:ON,limit to 5000mA @16:02:48.60 Gate.power :analytic1,:ON,limit to 5000mA @16:02:48.61 Gate.power :analytic2,:ON,limit to 5000mA @16:02:48.69 Powering core bus @16:02:51.05 SPR Module 1, FW Rev. B, FPGA Rev. C @16:02:51.47
Archive.configure :default @16:02:51.56 Air.configure :default @16:02:51.62 Lysis1.configure :default @16:02:51.70 Lysis2.configure :default @16:02:51.82 Plunge.configure :default @16:02:51.91 SamplePump.configure :default @16:02:52.01 Toroid.configure :default @16:02:52.10 Carousel.configure :default @16:02:52.19 Pressure.configure :default @16:02:52.29 ExtraPump.configure :default @16:02:52.35 PinPort.configure :default @16:02:52.41 All elf controllers running firmware version 4.84 @16:02:52.43 Gate.power :motor,:ON,limit to 5000mA @16:02:52.77 Archive.home.jog 0 @16:02:53.99 PinPort.home.jog 0 @16:02:54.05 Carousel.home.jog 0 @16:02:54.08 Toroid.home.jog 0 @16:02:54.12 Lysis2.home.jog 0 @16:02:54.15 Lysis1.home.jog 0 @16:02:54.21 Plunge.home.jog 0 @16:02:54.26 Air.home.jog 0 @16:02:55.14 Archive.in :homing @16:02:55.35 Lysis2.in :homing @16:02:55.45 Archive.jog 1386 @16:02:55.48 PinPort.in :homing @16:02:55.51 Plunge.in :homing @16:02:55.55 Lysis1.in :homing @16:02:55.59 Toroid.in :homing @16:02:55.63 Air.in :homing @16:02:56.00 Lysis2.jog 1391 @16:02:56.04 PinPort.jog -107 @16:02:56.11 Plunge.jog 1573 @16:02:56.14 Lysis1.jog 1383 @16:02:56.19 Air.jog 1366 @16:02:56.25 Toroid.jog 609 @16:02:56.60 PinPort.setRawPosition! 0 @16:02:56.64 PinPort.seek :home @16:02:56.75 PinPort.in :default @16:02:56.97 Archive.setRawPosition! 50 @16:02:57.03 Archive.seek :home @16:02:57.32 Archive.in :default @16:02:57.39 Lysis2.setRawPosition! 50 @16:02:57.45 Lysis2.seek :home @16:02:57.56 Plunge.setRawPosition! 50 @16:02:57.64 Lysis1.setRawPosition! 50 @16:02:57.68 Air.setRawPosition! 50 @16:02:57.71 Plunge.seek :home @16:02:57.91 Lysis1.seek :home @16:02:57.94 Air.seek :home @16:02:58.10 Lysis2.in :default @16:02:58.21 Plunge.in :default @16:02:58.33 Lysis1.in :default @16:02:58.37 Air.in :default @16:02:58.53 Toroid.setRawPosition! 0 @16:02:58.58 Toroid.seek :home @16:02:58.68 Toroid.in :default @16:02:58.79
Toroid.seek :clear @16:03:00.40 Carousel.in :default @16:03:00.53 Carousel.home.seek 2 @16:03:00.80 Selecting Cartridge 1 @16:03:01.13 Carousel.seek 1 @16:03:01.66 Lysis1.in :fast @16:03:01.75 Lysis1.seek :locked @16:03:04.39 Plunge.seek :locked @16:03:04.56 Air.in :fast @16:03:04.63 Air.seek :locked @16:03:06.71
Carousel.reconfigure :locked @16:03:06.88 Powering cartridge bus @16:03:09.54 Gate.setIR 1750,5000,0,6 @16:03:09.66 Gate.setIR 0,5000,0,6 @16:03:09.76 PuckHeater.configure :default #ambient 25.4C @16:03:09.83 Gate.power :heat,:ON,limit to 5000mA @16:03:10.55 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @16:03:10.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:03:10.93 Preparing to filter cartridge 1 @16:03:11.17 Gate.power :sampler,:ON @16:03:11.26 Priming sample loop w/150ml, bypass w/50ml @16:03:11.28 SamplePump.setPosition! 0ml @16:03:11.32 Bypass.open @16:03:12.86 Intake.open @16:03:13.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:03:16.17 Vacuum tare is 56% of full scale @16:03:16.33 Pressure.in :default @16:03:16.39 Pressure.hold @16:03:16.62 Waiting for external sampler to finish priming... @16:03:16.63 delayUntil "16:06:41.19" @16:06:41.24 SamplePump.in :prime @16:06:41.31 SamplePump.seek 50ml,2:53 @16:07:42.29 Pressure.stop @16:07:42.34 SamplePump.setPosition! 0ml @16:07:42.40 Exhaust.open @16:07:43.86 Bypass.close @16:07:45.40 Pressure.in :default @16:07:45.47 Pressure.hold @16:07:45.80 SamplePump.in :prime @16:07:45.87 SamplePump.seek 150ml,7:39 @16:10:47.93 Pressure.stop @16:10:48.01 Bypass.open @16:10:48.72 Intake.close @16:10:49.50 Toroid.in :hold @16:10:49.57 Toroid.seek :crack @16:10:51.11 Toroid.seek :cartridge @16:10:52.22 Toroid.in :default @16:10:52.69 Toroid.seek :clear @16:10:54.81 Pressurizing cartridge to 26psi above 14.6psia @16:10:54.83 SamplePump.setPosition! 0ml @16:10:54.87 Intake.open @16:10:55.56 Bypass.close @16:10:56.26 Exhaust.close @16:10:57.10 Pressure.hold @16:10:57.35 SamplePump.in :charge @16:10:57.42 SamplePump.seek 2.3ml,42 seconds @16:11:11.65 Pressure.stop @16:11:11.71 Intake.close @16:11:12.50 Pressurized cartridge with 0.918ml to 26.2psi @16:11:12.51 Isolated @35.2psia (20.5psi) @16:11:12.53 Verifying cartridge holds at least 0.65..0.85 of 35.2psia @16:11:12.55 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:11:17.23 Sampling 50.0ml @16:11:17.25 SamplePump.setPosition! 0ml @16:11:17.30 Exhaust.open @16:11:18.01 Intake.open @16:11:18.83 Pressure.in :default @16:11:18.89 Pressure.hold @16:11:19.04 Cannot pump 0.85ml/s -- Limiting to 0.82ml/s @16:11:19.24 SamplePump.in :sample @16:11:19.31 SamplePump.seek 50ml,4:41 @16:11:22.36 Cartridge 1 filtering 2/50ml at 0.59ml/s with 29.4psi vacuum @16:12:22.36 Cartridge 1 filtering 32/50ml at 0.50ml/s with 23.9psi vacuum @16:13:00.00 Pressure.stop @16:13:00.15 Intake.close @16:13:00.21 Exhaust.close @16:13:00.43 Toroid.in :hold @16:13:00.49 Toroid.seek :bypass @16:13:02.13 Toroid.in :default @16:13:02.20 Toroid.seek :clear @16:13:04.32 Sampled 50.0ml @16:13:06.04 SPR.command "read_sequences" @16:13:06.66 SPR.command "stats_on" @16:13:06.83 SPR.command "start 0 1" @16:13:07.45 Gate.power :sampler,:OFF @16:13:07.53 SPR.start /var/log/esp/spr23aug1616h.spr.bz2 @16:13:09.50 Handoff.select :waste @16:13:16.22 Syringe tare is 18% of full scale @16:13:16.25 PinPort.in :locking @16:13:16.31 PinPort.seek :locked @16:13:18.85 Archive.in :fast @16:13:18.92 Lysis2.in :fast @16:13:18.96 Lysis1.seek 1350uL @16:13:19.14 Archive.seek :full @16:13:19.25 Lysis2.seek 950uL @16:13:23.95 Plunge.seek :reagent @16:13:24.86 Evacuating puck with filter bubble point of 50psi @16:13:24.89 PinPort.seek :locked @16:13:24.97 Handoff.select :waste @16:13:31.58 Air syringe pumping up to 1 strokes to reach 8psi @16:13:31.59 Air stroke #1 of 1 @16:13:31.68 Air.in :pressurize @16:13:31.81 Air.seek :pressurized @16:13:37.55 1.7psi after pressurize @16:13:37.57 Air.in :compress @16:13:37.64 Air.seek :evac @16:13:40.66 1.4psi after compress @16:13:51.26 0.2psi after compress (stable) @16:13:51.28 Air.in :default @16:13:51.34 Air.hold @16:13:51.57 at 0.2psi Opened to waste @16:13:51.59 Handoff.select :blocked @16:13:51.77 Archive syringe pumping up to 8 strokes to reach 50psi @16:13:51.78 Archive stroke #1 of 8 @16:13:51.87 Archive.in :pressurize @16:13:51.94 Archive.seek :pressurized @16:14:04.84 10.8psi after pressurize @16:14:13.54 12.1psi after pressurize (stable) @16:14:13.57 Archive.in :compress @16:14:13.63 Archive.seek :empty @16:14:28.25 29.3psi after compress @16:14:41.86 33.5psi after compress (stable) @16:14:41.88 Archive.in :gulp @16:14:41.95 Archive.seek :topVent @16:14:48.21 34.0psi after gulp @16:14:53.83 33.8psi after gulp (stable) @16:14:53.84 Archive stroke #2 of 8 @16:14:53.94 Archive.in :pressurize @16:14:54.01 Archive.seek :pressurized @16:15:06.56 33.0psi after pressurize @16:15:13.27 33.6psi after pressurize (stable) @16:15:13.29 Archive.in :compress @16:15:13.35 Archive.seek :empty @16:15:27.97 Reached 50.2 of 50.0psi after pumping 3459uL (2.00 strokes) @16:15:27.99 Archive.in :default @16:15:28.05 Archive.hold @16:15:28.19 Handoff.select :waste @16:15:38.90 delay 1:00 @16:16:38.92 Handoff.select :blocked @16:17:01.64 Handoff.select :waste @16:17:01.79 0.7psi at evacEnd @16:17:01.80 Handoff.select :blocked @16:17:01.94 Archive.in :fast @16:17:02.00 Archive.seek :topVent @16:17:15.68 Stable @0.8psi after Handoff blocked @16:17:15.71 Archive syringe pumping up to 8 strokes to reach 31psi @16:17:15.72 Archive stroke #1 of 8 @16:17:15.84 Archive.in :pressurize @16:17:15.92 Archive.seek :pressurized @16:17:28.45 2.3psi after pressurize @16:17:28.48 Archive.in :compress @16:17:28.54 Archive.seek :empty @16:17:43.58 15.5psi after compress @16:17:54.29 16.2psi after compress (stable) @16:17:54.32 Archive.in :gulp @16:17:54.39 Archive.seek :topVent @16:18:00.64 16.3psi after gulp @16:18:06.24 16.3psi after gulp (stable) @16:18:06.26 Archive stroke #2 of 8 @16:18:06.36 Archive.in :pressurize @16:18:06.42 Archive.seek :pressurized @16:18:18.92 16.2psi after pressurize @16:18:18.95 Archive.in :compress @16:18:19.01 Archive.seek :empty @16:18:33.63 30.0psi after compress @16:18:37.95 Reached 30.8 of 30.8psi after pumping 3460uL (2.00 strokes) @16:18:37.97 Archive.in :default @16:18:38.03 Archive.hold @16:18:38.10 Handoff.select :waste @16:18:45.33 delay 1:00 @16:19:45.36 Handoff.select :blocked @16:20:08.11 Handoff.select :waste @16:20:08.25 0.4psi at evacEnd @16:20:08.28 Air.seek :fullVent @16:20:09.70 Archive.in :fast @16:20:09.79 Archive.seek :topVent @16:20:16.88 Seawater evacuated, Pressure above/below filter released. @16:20:16.98 Lysis1.in :slow @16:20:17.05 Lysis1.seek 1050uL @16:20:47.05 delay 5 seconds @16:20:52.07 Handoff.select :blocked @16:20:52.26 Lysis1.seek 450uL @16:21:52.18 delay 3 seconds @16:21:55.29 Air.in mix with 1.40048 parts Lysis1 @16:21:55.95 Air.seek :evac @16:21:56.05 Lysis1.seek 300uL @16:22:11.13 Lysis1.seek :empty @16:22:41.22 Lysis buffer delivered @38.3psi @16:22:41.24 Polling Can every 2:00 @16:22:41.79 PuckHeater.configure :default #ambient 26.0C @16:22:41.85 PuckHeater.seek 79.6C @16:23:23.07 PuckHeater.hold 2:00,79.6C @16:25:22.76 Polling Can every 2:00 @16:25:23.31 PuckHeater.configure :default #ambient 26.1C @16:25:23.36 PuckHeater.hold 30 seconds,78.6C @16:25:53.47 Polling Can every 2:00 @16:25:54.02 PuckHeater.hold 30 seconds,78C @16:26:24.13 Polling Can every 2:00 @16:26:24.68 PuckHeater.configure :default #ambient 26.0C @16:26:24.75 PuckHeater.hold 10:00,77.5C @16:36:23.01 Lysis complete. Syringe pressure @44.6psi @16:36:23.03 Archive syringe pumping up to 5 strokes to reach 40psi @16:36:23.05 Archive stroke #1 of 5 @16:36:23.21 Archive.in :pressurize @16:36:23.31 Archive.seek :pressurized @16:36:23.56 Reached 44.6 of 40.0psi after pumping 0uL (0.00 strokes) @16:36:23.58 Archive.in :default @16:36:23.64 Archive.hold @16:36:23.75 delay 2 seconds @16:36:25.79 Archive.in :fast @16:36:25.85 Archive.seek :full @16:36:26.13 Lysis1.seek 138uL @16:36:40.02 delay 1 second @16:36:41.05 Archive.seek :full @16:36:41.09 Lysis1.seek :empty @16:36:55.04 Handoff.select :blocked @16:36:55.17 Initial air pressure @47.4psi @16:36:55.26 Lysis1.in :extract @16:36:55.37 Lysis1.seek 600uL,nil @16:36:57.11 Archive syringe pumping up to 6 strokes to reach 25psi @16:36:57.12 Archive stroke #1 of 6 @16:36:57.23 Archive.in :pressurize @16:36:57.35 Archive.seek :pressurized @16:37:09.91 10.9psi after pressurize @16:37:15.51 10.8psi after pressurize (stable) @16:37:15.53 Archive.in :compress @16:37:15.60 Archive.seek :empty @16:37:24.59 Archive.in :default @16:37:24.66 Archive.hold @16:37:24.89 Initial 600uL lysate @17.8psi @16:37:24.90 Handoff.select :blocked @16:37:25.00 Initial air pressure @18.2psi @16:37:25.09 Lysis1.seek 825uL,nil @16:37:26.40 Archive syringe pumping up to 6 strokes to reach 17psi @16:37:26.43 Archive stroke #1 of 6 @16:37:26.54 Archive.in :compress @16:37:26.67 Archive.seek :empty @16:37:28.40 Archive.in :default @16:37:28.49 Archive.hold @16:37:28.82 Recovered 826uL lysate @15.5psi @16:37:28.84 Air.in :halfSpeed @16:37:28.91 Air.seek :fullVent @16:37:31.13 Puck Top Vented, bottom @9.6psi @16:37:31.14 Handoff.select :waste @16:37:31.27 delay 5 seconds @16:37:36.37 Handoff open to waste, bottom @0.1psi @16:37:36.39 Air.in :default @16:37:36.45 Air.seek :evac @16:37:37.87 Lysis2.in :DAmix @16:37:38.06 Lysis1.in :DAmix @16:37:38.62 Lysis2.seek 700uL @16:37:38.67 Lysis1.seek 576uL @16:38:13.76 delay 5 seconds @16:38:18.79 Handoff.select SPR @16:38:18.92 SPR.command "start 2 14" @16:38:20.00 SPR stabilized @16:38:20.17 delay 10 seconds @16:38:30.19 SPR.command "start 2 0" @16:38:33.37 Waiting up to 11:00 for SPR to enter state 1 @16:40:31.04 SPR.command "hold_on" @16:40:32.43 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:40:33.08 Lysis2.seek 400uL @16:40:33.18 Lysis1.seek 276uL @16:41:15.14 delay 15 seconds @16:41:30.16 SPR.command "hold_off" @16:41:33.08 Waiting up to 38:00 for SPR to enter state 7 @16:55:53.27 SPRsummary:-3.82RIU,-12.3RIU,79.1RIU,97.9RIU,high,179ng/L,304ng/L after 2710s @17:07:23.69 SPR analysis completed @17:07:30.30 Ended log spr23aug1616h.spr.bz2 @17:07:30.31 SPR.command "stats_off" @17:07:30.93 Handoff.select :close @17:07:31.09
Gate.power :heat,:OFF @17:07:31.10 Gate.power :cartridge,:OFF @17:07:31.19 PinPort.in :unlocking @17:07:31.26 PinPort.seek :clear @17:07:33.17 Carousel.in :default @17:07:33.31 Carousel.home.seek 1 @17:07:33.66 Plunge.seek :locked @17:07:34.57 Archive.in :unlocking @17:07:34.73 Archive.seek :clear @17:07:34.80 Air.in :unlocking @17:07:35.01 Air.seek :clear @17:07:35.08 Lysis1.in :unlocking @17:07:35.27 Lysis1.seek :clear @17:07:35.33 Lysis2.in :unlocking @17:07:35.47 Lysis2.seek :clear @17:07:39.41
Gate.power :motor,:OFF @17:07:39.42 Gate.power :core,:OFF @17:07:39.43 Gate.power :analytic1,:OFF @17:07:39.45 Gate.power :analytic2,:OFF @17:07:39.46 Gate.power :main,:OFF @17:07:39.47 Safely stopped and ready to power off @17:07:39.67 Cartridge::None in MAIN -- Not enough dry cartridges remain @19:04:58.21PDT16-Aug-23
Environmental Sample Processor built 24-Jul-23 (df8af88d) @19:04:59.86 >BREAK< ESP3 Gateway boot v1.43 for core bus @19:04:59.89 >BREAK< ESP3 Gateway boot v1.43 for cartridge bus @19:05:03.28 Configuration for honu by nadia@mbari.org 7/20/23 /home/esp/esp2/type/auv/honu/configure.rb @19:05:03.93 Sea pressure (Abs) tare is 6% of full scale @19:05:17.12 Polling Can every 2:00 @19:05:17.16 Waiting for espclients... @19:05:24.33 Session Begun @19:05:26.20 -> slot {1 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 826, spr: "spr23aug1616h.spr.bz2"}, 2 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1615h.spr.bz2"}, 3 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 100}, 4 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1612h.spr.bz2"}, [56,54,11,5] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 6 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1608h.spr.bz2"}, 7 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 227.5}, 8 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1606h.spr.bz2"}, 9 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 309.2}, 10 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1604h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @19:24:50.88 -> ESP.ready! @19:24:50.94 Gate.power :sampler,:OFF,limit to 3500mA @19:24:50.96 Gate.power :main,:ON,limit to 5000mA @19:24:51.00 Gate.power :analytic1,:ON,limit to 5000mA @19:24:51.04 Gate.power :analytic2,:ON,limit to 5000mA @19:24:51.22 Powering core bus @19:24:53.49 SPR Module 1, FW Rev. B, FPGA Rev. C @19:24:54.00 Archive.configure :default @19:24:54.12 Air.configure :default @19:24:54.21 Lysis1.configure :default @19:24:54.30 Lysis2.configure :default @19:24:54.42 Plunge.configure :default @19:24:54.54 SamplePump.configure :default @19:24:54.67 Toroid.configure :default @19:24:54.79 Carousel.configure :default @19:24:54.91 Pressure.configure :default @19:24:55.02 ExtraPump.configure :default @19:24:55.11 PinPort.configure :default @19:24:55.20 All elf controllers running firmware version 4.84 @19:24:55.23 Gate.power :motor,:ON,limit to 5000mA @19:24:55.54 Archive.home.jog 0 @19:24:56.33 Carousel.home.jog 0 @19:24:56.39 Toroid.home.jog 0 @19:24:56.43 Plunge.home.jog 0 @19:24:56.46 Lysis2.home.jog 0 @19:24:56.50 Air.home.jog 0 @19:24:56.54 PinPort.home.jog 0 @19:24:56.57 Lysis1.home.jog 0 @19:24:57.40 Archive.in :homing @19:24:57.67 Archive.jog 1385 @19:24:57.79 Toroid.in :homing @19:24:57.84 Lysis1.in :homing @19:24:57.90 PinPort.in :homing @19:24:57.96 Air.in :homing @19:24:58.02 Lysis2.in :homing @19:24:58.08 Plunge.in :homing @19:24:58.89 Lysis1.jog 1384 @19:24:58.97 Toroid.jog 611 @19:24:59.01 PinPort.jog -108 @19:24:59.06 Air.jog 1367 @19:24:59.10 Plunge.jog 1570 @19:24:59.15 Lysis2.jog 1392 @19:24:59.58 Archive.setRawPosition! 50 @19:24:59.61 PinPort.setRawPosition! 0 @19:24:59.78 Archive.seek :home @19:24:59.82 PinPort.seek :home @19:25:00.04 PinPort.in :default @19:25:00.16 Archive.in :default @19:25:00.37 Lysis1.setRawPosition! 50 @19:25:00.44 Lysis1.seek :home @19:25:00.57 Air.setRawPosition! 50 @19:25:00.66 Air.seek :home @19:25:00.82 Lysis2.setRawPosition! 50 @19:25:00.86 Plunge.setRawPosition! 50 @19:25:00.89 Lysis1.in :default @19:25:01.08 Lysis2.seek :home @19:25:01.15 Plunge.seek :home @19:25:01.37 Toroid.setRawPosition! 0 @19:25:01.40 Air.in :default @19:25:01.58 Toroid.seek :home @19:25:01.76 Plunge.in :default @19:25:01.79 Toroid.in :default @19:25:01.84 Lysis2.in :default @19:25:02.32 Toroid.seek :clear @19:25:03.95 Carousel.in :default @19:25:04.11 Carousel.home.seek 1 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 1 Pressure = Absolute Pressure (Leak) Sensor @15psia PinPort = Handoff Pin Port (PP) clear @19:25:30.64 -> SC.to 36 @19:25:30.68 Carousel.seek 36 Storage Carousel (SC) at 36 @19:25:47.29 -> Cartridge.lock @19:25:47.49 Lysis1.in :fast @19:25:47.58 Lysis1.seek :locked @19:25:50.24 Plunge.seek :locked @19:25:50.43 Air.in :fast @19:25:50.52 Air.seek :locked @19:25:52.12 Carousel.reconfigure :locked @19:25:52.31 Powering cartridge bus @19:25:54.98 Gate.setIR 1750,5000,0,6 @19:25:55.11 Gate.setIR 0,5000,0,6 @19:25:55.20 PuckHeater.configure :default @19:25:55.28 Gate.power :heat,:ON,limit to 5000mA @19:25:56.03 Mounted lysis Cartridge board#Q05045 @19:25:56.09 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @19:26:01.25 -> PP Handoff Pin Port (PP) clear @19:26:07.12 -> LyS1 Lysis Syringe #1 (LyS1) locked @19:26:17.69 -> LyS1.to 825 @19:26:17.74 Lysis1.in :default @19:26:17.86 Lysis1.seek 825uL Lysis Syringe #1 (LyS1) at 825uL @19:26:33.37 -> PP.cartridge @19:26:33.42 PinPort.in :locking @19:26:33.50 PinPort.seek :locked Handoff Pin Port (PP) cartridge @19:26:40.07 -> Handoff Handoff Valve::Manifold selects blocked @19:26:46.63 -> Handoff.waste @19:26:46.65 Handoff.select :waste Handoff Valve::Manifold selects waste @19:26:51.93 -> LyS2 Lysis Syringe #2 (LyS2) clear @19:26:58.00 -> LyS2.to 1000 @19:26:58.04 Lysis2.seek 1000uL Lysis Syringe #2 (LyS2) at 1000uL @19:34:54.79 Session Begun @19:34:57.43 -> slot 36 {36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: "spr23aug1107h.spr.bz2"}} @19:48:56.26 -> reload method :microSPRmanual /home/esp/esp2/protocol/3G/spr.rb @20:07:29.21 Session Ended @20:08:28.66 -> Cartridge.processingOptions {} @20:08:55.97 -> Lysis2 Lysis Syringe #2 (LyS2) at 1000uL @21:49:34.15 Session Begun @21:49:48.92 -> SPR.start; delay 2 @21:49:50.13 SPR.command "read_sequences" @21:49:50.31 SPR.command "stats_on" @21:49:50.41 delay 2 seconds @21:49:51.21 SPR.start /var/log/esp/spr23aug1621h.spr.bz2 @21:50:32.09 -> SPR.command "start 3 0"; delay '15:00' @21:50:32.12 SPR.command "start 3 0" @21:50:33.41 delay 15:00 @22:05:33.47 Errno::EPIPE in bill's_reader -- Broken pipe @22:05:39.48 Thread::Aborted in SPRlogger -- "Aborted by bill" @22:05:39.55 BadNews.email "\"Aborted by bill\"",Subject: "Thread::Aborted in SPRlogger" @22:05:49.46 upsync @22:08:54.42 Session Begun @22:09:00.91 -> who :who @22:09:22.70 -> exit @22:09:22.73 Session Ended @22:09:58.61 Session Begun @22:10:02.43 -> exit @22:10:02.46 Session Ended @22:10:06.52 Session Begun @22:10:19.56 -> SPR.stop @22:10:19.59 AnalyticModule::SPR::Error in bill -- not started @22:11:00.52 -> LyS1 Lysis Syringe #1 (LyS1) at 825uL @22:16:49.34 -> microSPRmanual @22:16:49.44 SPR.command "stats_on" @22:16:49.84 SPR.command "start 0 1" @22:16:50.49 SPR.start /var/log/esp/spr23aug1622h.spr.bz2 @22:16:51.48 Waiting for baseline SPR stabilization @22:16:51.50 Handoff.select :waste @22:16:58.13 Syringe tare is 19% of full scale @22:16:58.17 PinPort.seek :locked @22:16:58.34 Archive.in :fast @22:16:58.40 Lysis2.in :fast @22:16:58.51 Lysis1.in :fast @22:16:58.80 Lysis1.seek 825uL @22:16:58.83 Lysis2.seek :full @22:16:58.91 Archive.seek :full @22:17:02.71 delayUntil "22:26:51.05" @22:18:31.60 Session Begun @22:18:52.84 -> SPR.command "start 2 14" @22:18:52.86 SPR.command "start 2 14" @22:19:02.76 -> SPR.stop @22:19:03.48 Ended log spr23aug1622h.spr.bz2 @22:19:03.50 SPR.command "stats_off" @22:19:22.75 -> LyS2 Lysis Syringe #2 (LyS2) locked @22:19:32.20 -> LyS2.to 1000 @22:19:32.24 Lysis2.in :default @22:19:32.85 Lysis2.seek 1000uL Lysis Syringe #2 (LyS2) at 1000uL @22:20:40.88 -> microSPRmanual lysed2:Lysis2.volume @22:20:41.15 SPR.command "stats_on" @22:20:41.65 SPR.command "start 0 1" @22:20:42.33 SPR.start /var/log/esp/spr23aug1622h#2.spr.bz2 @22:20:44.16 Waiting for baseline SPR stabilization @22:20:44.18 Handoff.select :waste @22:20:50.85 Syringe tare is 19% of full scale @22:20:50.93 PinPort.seek :locked @22:20:51.09 Archive.seek :full @22:20:51.25 Lysis2.in :fast @22:20:51.37 Lysis1.seek 825uL @22:20:51.59 Lysis2.seek 1000uL @22:20:51.85 delayUntil "22:30:43.73" @22:26:51.06 Lysis2.in :DAmix @22:26:51.31 Lysis1.in :DAmix @22:26:52.06 Lysis2.seek 750uL @22:26:52.15 Lysis1.seek 575uL @22:27:27.28 delay 5 seconds @22:27:32.31 Handoff.select SPR @22:27:32.52 SPR.command "start 2 14" @22:27:33.71 SPR stabilized @22:27:33.97 delay 10 seconds @22:27:43.99 SPR.command "start 2 0" @22:27:47.07 Waiting up to 11:00 for SPR to enter state 1 @22:29:44.75 SPR.command "hold_on" @22:29:46.13 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:29:46.84 Lysis2.seek 450uL @22:29:46.92 Lysis1.seek 275uL @22:30:29.02 delay 15 seconds @22:30:44.36 Lysis2.seek 200uL @22:30:44.41 Lysis1.seek 25uL @22:30:44.64 SPR.command "hold_off" @22:30:47.73 Waiting up to 38:00 for SPR to enter state 7 @22:31:19.56 delay 5 seconds @22:31:24.58 Handoff.select SPR @22:31:24.61 SPR.command "start 2 14" @22:31:25.44 SPR stabilized @22:31:25.60 Additional SPR Analyses Skipped due to Insufficient Volume @22:31:26.90 SPR analysis completed @22:31:30.99 Ended log spr23aug1622h#2.spr.bz2 @22:31:31.01 SPR.command "stats_off" @22:32:55.99 -> SPR.start @22:32:56.12 SPR.command "stats_on" # @22:32:57.18 SPR.start /var/log/esp/spr23aug1622h#3.spr.bz2 @22:41:12.87 -> SPR.command "start 2 5" @22:41:12.89 SPR.command "start 2 5" @22:41:28.51 Ended log spr23aug1622h#3.spr.bz2 @22:41:28.53 SPR.command "stats_off" @22:41:47.11 -> SPR.start @22:41:47.21 SPR.command "stats_on" # @22:41:48.59 SPR.start /var/log/esp/spr23aug1622h#4.spr.bz2 @22:42:07.25 -> SPR.command "start 2 6" @22:42:07.28 SPR.command "start 2 6" @23:13:59.98 Session Ended @23:14:23.38 -> SPR.command "2 14" @23:14:23.41 SPR.command "2 14" @23:14:25.27 AnalyticModule::SPR::Error in bill -- response to command "2 14" was "%\"2 14\": invalid command" (try 1 of 3) @23:14:27.57 AnalyticModule::SPR::Error in bill -- response to command "2 14" was "%\"2 14\": invalid command" (try 2 of 3) @23:14:30.41 AnalyticModule::SPR::Error in bill -- response to command "2 14" was "%\"2 14\": invalid command" @23:14:36.22 -> SPR.stop @23:14:51.02 Ended log spr23aug1622h#4.spr.bz2 @23:14:51.04 SPR.command "stats_off" @23:15:00.73 -> SPR.start @23:15:00.81 SPR.command "stats_on" # @23:15:02.41 SPR.start /var/log/esp/spr23aug1623h.spr.bz2 @23:15:14.62 -> SPR.command "start 2 14" @23:15:14.64 SPR.command "start 2 14" @23:15:19.68 -> SPR.stop @23:15:19.89 Ended log spr23aug1623h.spr.bz2 @23:15:19.93 SPR.command "stats_off" @23:15:32.96 -> exit @23:15:32.99 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @23:16:58.36 Session Begun @23:17:02.31 -> who :who @23:17:11.58 -> slot {1 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 826, spr: "spr23aug1616h.spr.bz2"}, 2 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1615h.spr.bz2"}, 3 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 100}, 4 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1612h.spr.bz2"}, [56,54,11,5] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 6 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1608h.spr.bz2"}, 7 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 227.5}, 8 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1606h.spr.bz2"}, 9 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 309.2}, 10 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug1604h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1511h.spr.bz2"}, 13 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 418.7}, 14 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1508h.spr.bz2"}, 15 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 382.4}, 16 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug1413h.spr.bz2"}, 17 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 192.6}, 18 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1408h.spr.bz2"}, 19 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 303.9}, 20 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1313h.spr.bz2"}, 21 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 365.4}, 22 => { Type: :microSPR_phyto, State: :leaky}, 23 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 205.6}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1310h.spr.bz2"}, 25 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 286.3}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 827, spr: "spr23aug1307h.spr.bz2"}, 27 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 323.6}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1211h.spr.bz2"}, 29 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 369.8}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1208h.spr.bz2"}, 31 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 380.9}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1315h.spr.bz2"}, 33 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 169.6}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1110h.spr.bz2"}, 35 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 133.2}, 36 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, lysed: 825, spr: spr23aug1622h#2.spr.bz2}, 37 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 134.8}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug1009h.spr.bz2"}, 39 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 689.3}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0915h.spr.bz2"}, 41 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 495.8}, 42 => { Type: :microSPR_phyto, State: :lysing, filtered: 50, spr: "spr23aug0908h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 439.1}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0906h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 542.5}, 46 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0808h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 480.6}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 50, lysed: 825, spr: "spr23aug0714h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 446.1}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1414h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1516h#2.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1416h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23aug1021h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr23aug0707h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0704h.spr.bz2"}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000, was: :dry}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0700h.spr.bz2", was: :dry}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr23aug0623h.spr.bz2", was: :dry}} @23:17:40.44 -> Cmd.stop @23:17:40.61 Handoff.select :close @23:17:40.81 Gate.power :heat,:OFF @23:17:40.84 Gate.power :cartridge,:OFF @23:17:40.99 PinPort.in :unlocking @23:17:41.10 PinPort.seek :clear @23:17:43.07 Carousel.in :default @23:17:43.80 Carousel.home.seek 36 @23:17:44.23 Plunge.seek :locked @23:17:44.47 Archive.in :unlocking @23:17:44.66 Archive.seek :clear @23:17:44.88 Air.in :unlocking @23:17:45.11 Air.seek :clear @23:17:45.26 Lysis1.in :unlocking @23:17:45.50 Lysis1.seek :clear @23:17:45.61 Lysis2.in :unlocking @23:17:45.81 Lysis2.seek :clear @23:17:50.16 Gate.power :motor,:OFF @23:17:50.23 Gate.power :core,:OFF @23:17:50.25 Gate.power :analytic1,:OFF @23:17:50.29 Gate.power :analytic2,:OFF @23:17:50.34 Gate.power :main,:OFF @23:17:50.40 Safely stopped and ready to power off :STOPPED @23:18:05.93 -> MainThread.exit # @23:18:06.10 Session Ended @23:18:06.16 Session Ended