@12:19:53.54PDT16-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:19:55.26 >BREAK< ESP3 Gateway v1.43 for core bus @12:19:55.28 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:19:59.54 Configuration for honu by nadia@mbari.org 7/3/24 /home/esp/esp2/type/auv/honu/configure.rb @12:20:00.23 Sea pressure (Abs) tare is 6% of full scale @12:20:03.75 Polling Can every 2:00 @12:20:03.80 Waiting for espclients... @12:20:03.94 Session Begun @12:20:12.37 -> Cartridge.sampler.source @12:20:12.41 Cartridge::Error in n -- No Cartridge mounted @12:20:16.65 -> Cartridge.lock @12:20:16.70 Gate.power :heat,:OFF @12:20:17.08 Carousel.reconfigure :locked @12:20:17.31 Gate.power :sampler,:OFF,limit to 3500mA @12:20:17.34 Gate.power :skimmer,:OFF,limit to 3500mA @12:20:17.38 Gate.power :analytic1,:ON,limit to 4000mA @12:20:17.85 Gate.power :cartridge,:OFF @12:20:18.18 Plunge.configure :default @12:20:18.29 Plunge.hold @12:20:18.40 Plunge.configure :default @12:20:18.58 SamplePump.configure :default @12:20:18.76 Toroid.configure :default @12:20:18.84 Toroid.hold @12:20:18.95 Toroid.configure :default @12:20:19.13 Carousel.hold @12:20:19.25 Carousel.configure :locked @12:20:19.67 Abs.configure :default @12:20:19.84 PhaseSeparator.configure :default @12:20:20.03 Archive.configure :default @12:20:20.24 Air.configure :default @12:20:20.32 Air.hold @12:20:20.45 Air.configure :default @12:20:20.60 Lysis1.configure :default @12:20:20.68 Lysis1.hold @12:20:20.80 Lysis1.configure :default @12:20:20.95 Lysis2.configure :default @12:20:21.38 PinPort.configure :default @12:20:21.48 All elf controllers running firmware version 4.92 @12:20:22.88 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:20:22.91 AnalyticModule::Unknown in n -- SPR @12:20:23.09 Powering cartridge bus @12:20:26.75 Gate.setIR 1750,5000,0,6 @12:20:26.87 Gate.setIR 0,5000,0,6 @12:20:26.97 PuckHeater.configure :default @12:20:27.06 Gate.power :heat,:ON,limit to 5000mA @12:20:27.83 Mounted lysis Cartridge board#Q05092 @12:20:27.88 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:20:29.88 -> Cartridge.sampler.source :sampler @12:20:41.22 -> Cartridge.sampler.source=nil @12:20:48.71 -> slots {[54,43,34,28,15,6] => { Type: :lyseHold_phyto, State: :dry}, [31,30] => { Type: :archive_bac, State: :missing}} @12:21:04.11 -> to 53 @12:21:04.34 Gate.power :heat,:OFF @12:21:04.38 Gate.power :cartridge,:OFF @12:21:04.48 Carousel.in :default @12:21:04.64 Carousel.home.seek 54 @12:21:05.10 Air.in :unlocking @12:21:05.24 Air.seek :clear @12:21:06.27 Lysis1.in :unlocking @12:21:06.45 Lysis1.seek :clear @12:21:07.01 Plunge.in :unlocking @12:21:07.16 Plunge.seek :clear @12:21:08.41 Carousel.seek 53 @12:21:08.98 Lysis1.in :fast @12:21:09.06 Lysis1.seek :locked @12:21:11.74 Plunge.in :default @12:21:11.87 Plunge.seek :locked @12:21:12.02 Air.in :fast @12:21:12.12 Air.seek :locked @12:21:13.13 Carousel.reconfigure :locked @12:21:13.31 Powering cartridge bus @12:21:15.99 Gate.setIR 1750,5000,0,6 @12:21:16.12 Gate.setIR 0,5000,0,6 @12:21:16.23 Gate.power :cartridge,:OFF @12:21:16.79 Mounted archive Cartridge :archive_bac @12:21:25.31 -> Cartridge.sampler.source=nil @12:21:31.44 -> Cartridge.unlock @12:21:31.56 Carousel.in :default @12:21:31.70 Carousel.home.seek 53 @12:21:32.16 Air.in :unlocking @12:21:32.33 Air.seek :clear @12:21:33.39 Lysis1.in :unlocking @12:21:33.56 Lysis1.seek :clear @12:21:33.66 Plunge.in :unlocking @12:21:33.77 Plunge.seek :clear @12:21:48.01 -> fire lysis @12:21:48.22 Cartridge::None in detached from n -- No dry microSPR_phyto cartridges remain # @12:21:48.32 BadNews.email "No dry microSPR_phyto cartridges remain",Subject: "Cartridge::None in detached from n" @12:21:52.24 upsync @12:22:26.17 -> fire lyseHold_phyto # @12:22:27.20 Toroid.seek :clear @12:22:27.40 Carousel.in :default @12:22:27.55 Carousel.home.seek 53 @12:22:27.82 Selecting Cartridge 54 @12:22:28.20 Carousel.seek 54 @12:22:29.21 Lysis1.in :fast @12:22:29.30 Lysis1.seek :locked @12:22:32.04 Plunge.in :default @12:22:32.16 Plunge.seek :locked @12:22:32.35 Air.in :fast @12:22:32.43 Air.seek :locked @12:22:33.43 Carousel.reconfigure :locked @12:22:33.62 Powering cartridge bus @12:22:36.28 Gate.setIR 1750,5000,0,6 @12:22:36.41 Gate.setIR 0,5000,0,6 @12:22:36.51 PuckHeater.configure :default @12:22:36.61 Gate.power :heat,:ON,limit to 5000mA @12:22:37.30 Mounted lysis Cartridge board#Q05092 @12:22:37.36 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:22:37.41 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 54" @12:22:37.81 Preparing to filter cartridge 54 @12:22:38.26 Air.in :default @12:22:38.45 Air.seek :sealed @12:22:38.62 Priming sample loop w/150ml, bypass w/50ml @12:22:38.66 SamplePump.setPosition! 0ml @12:22:38.84 Exhaust.close @12:22:39.02 Bypass.open @12:22:40.35 upsync @12:22:40.60 Intake.open @12:22:41.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:22:44.03 Vacuum tare is 52% of full scale at 22C (drifted 1%) @12:22:44.27 Abs.in :default @12:22:44.35 Abs.hold @12:22:44.64 SamplePump.in :start @12:22:44.75 SamplePump.seek 50ml,3:50 @12:22:45.34 SamplePump.configure :prime @12:23:46.16 Abs.stop @12:23:46.25 SamplePump.setPosition! 0ml @12:23:46.34 Exhaust.open @12:23:47.75 Bypass.close @12:23:49.30 Abs.in :default @12:23:49.41 Abs.hold @12:23:50.34 SamplePump.in :start @12:23:50.44 SamplePump.seek 150ml,10:30 @12:23:51.03 SamplePump.configure :prime @12:26:53.85 Abs.stop @12:26:53.97 Bypass.open @12:26:54.65 Intake.close @12:26:55.46 Toroid.in :hold @12:26:55.55 Toroid.seek :crack @12:26:57.14 Toroid.seek :cartridge @12:26:58.29 Toroid.in :default @12:26:58.38 Toroid.seek :clear @12:27:00.46 Pressurizing cartridge to 26psi above 15.7psia @12:27:00.49 SamplePump.setPosition! 0ml @12:27:00.58 Intake.open @12:27:01.25 Bypass.close @12:27:01.92 Exhaust.close @12:27:02.72 Abs.in :default @12:27:02.80 Abs.hold @12:27:03.06 SamplePump.in :charge @12:27:03.17 SamplePump.seek 2.3ml,42 seconds @12:27:15.29 SamplePump.stop @12:27:15.39 Intake.close @12:27:15.64 Toroid.in :hold @12:27:15.75 Toroid.seek :bypass @12:27:17.43 Toroid.in :default @12:27:17.53 Toroid.seek :clear @12:27:19.19 Abs.stop @12:27:19.49 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only 0.7psi vacuum after 0.7ml @12:27:19.52 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @12:27:19.57 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only 0.7psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @12:27:22.20 upsync @12:48:53.14 -> Cartridge.unlock @12:48:53.20 Gate.power :heat,:OFF @12:48:53.22 Gate.power :cartridge,:OFF @12:48:53.35 Carousel.in :default @12:48:53.50 Carousel.home.seek 54 @12:48:53.98 Air.in :unlocking @12:48:54.17 Air.seek :clear @12:48:56.25 Lysis1.in :unlocking @12:48:56.41 Lysis1.seek :clear @12:48:56.54 Plunge.in :unlocking @12:48:56.63 Plunge.seek :clear @12:50:05.26 -> fire lyseHold_phyto @12:50:05.45 Selecting Cartridge 43 # @12:50:05.83 Carousel.seek 43 @12:50:08.44 Lysis1.in :fast @12:50:08.53 Lysis1.seek :locked @12:50:11.19 Plunge.in :default @12:50:11.35 Plunge.seek :locked @12:50:11.48 Air.in :fast @12:50:11.58 Air.seek :locked @12:50:12.59 Carousel.reconfigure :locked @12:50:12.77 Powering cartridge bus @12:50:15.43 Gate.setIR 1750,5000,0,6 @12:50:15.57 Gate.setIR 0,5000,0,6 @12:50:15.66 PuckHeater.configure :default @12:50:15.76 Gate.power :heat,:ON,limit to 5000mA @12:50:16.48 Mounted lysis Cartridge board#Q05075 @12:50:16.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:50:16.59 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 43" @12:50:17.03 Preparing to filter cartridge 43 @12:50:17.74 Air.in :default @12:50:17.90 Bypass.open @12:50:18.05 Air.seek :sealed @12:50:18.30 Cmd::Error in detached from n -- Cannot process while in PRIMING state @12:50:18.33 Waiting to Resume from Checkpoint "Selecting Cartridge 54" @12:50:18.79 Toroid.in :hold @12:50:18.87 Toroid.seek :crack @12:50:19.26 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 54" @12:50:20.26 upsync @12:50:21.12 Toroid.seek :cartridge @12:50:21.42 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n" @12:50:22.19 upsync @12:50:22.49 Toroid.in :default @12:50:22.62 Toroid.seek :clear @12:50:23.91 upsync @12:50:24.48 Exhaust.open @12:50:25.43 Pressurizing cartridge to 26psi above 15.6psia @12:50:25.46 SamplePump.setPosition! 0ml @12:50:25.56 Intake.open @12:50:26.24 Bypass.close @12:50:26.89 Exhaust.close @12:50:27.70 Abs.in :default @12:50:27.79 Abs.hold @12:50:28.06 SamplePump.seek 2.3ml,42 seconds @12:50:44.33 Abs.stop @12:50:44.44 Intake.close @12:50:45.16 Pressurized cartridge with 1.187ml to 26.0psi @12:50:45.19 Isolated @38.6psia (23.1psi) @12:50:45.23 Verifying cartridge holds at least 0.65..0.85 of 38.6psia @12:50:45.29 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:50:49.92 Cartridge pressure fell 3% to 37.3psia @12:50:50.06 Sampling 50.0ml @12:50:50.09 SamplePump.setPosition! 0ml @12:50:50.24 Exhaust.open @12:50:50.90 Intake.open @12:50:51.76 Abs.in :default @12:50:51.85 Abs.hold @12:50:52.27 SamplePump.in :start @12:50:52.38 SamplePump.seek 50ml,4:40 @12:50:52.96 SamplePump.configure :sample @12:50:55.37 Cartridge 43 filtering 2/50ml at 0.74ml/s with 22.2psi vacuum 4.4m deep (21.2psia) @12:51:55.43 Cartridge 43 filtering 40/50ml at 0.64ml/s with 14.0psi vacuum @12:52:10.73 Abs.stop @12:52:11.47 Intake.close @12:52:11.58 Exhaust.close @12:52:11.82 Toroid.in :hold @12:52:11.92 Toroid.seek :bypass @12:52:13.59 Toroid.in :default @12:52:13.70 Toroid.seek :clear @12:52:15.36 Sampled 50.0ml @12:52:15.81 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 43" @12:52:16.46 Handoff.select :waste @12:52:16.69 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:52:18.25 upsync @12:52:23.34 Syringe tare is 52% of full scale at 21C (drifted 1%) @12:52:23.37 PinPort.in :locking @12:52:23.48 PinPort.seek :locked @12:52:24.00 Archive.in :fast @12:52:24.05 Lysis2.in :fast @12:52:24.19 Lysis1.seek 1350uL @12:52:24.33 Archive.seek :full @12:52:24.47 Lysis2.seek :empty @12:52:32.53 Plunge.seek :reagent @12:52:33.47 Evacuating puck with filter bubble point of 50psi @12:52:33.50 PinPort.seek :locked @12:52:33.65 Handoff.select :waste @12:52:33.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:52:40.31 Air syringe pumping up to 1 strokes to reach 8psi @12:52:40.33 Air stroke #1 of 1 @12:52:40.95 Air.in :pressurize @12:52:41.03 Air.seek :pressurized @12:52:46.67 2.8psi after pressurize @12:52:46.70 Air.in :compress @12:52:46.82 Air.seek :evac @12:52:49.85 2.4psi after compress @12:52:51.18 Reached 8.0 of 8.0psi after pumping 1594uL (1.00 strokes) @12:52:51.20 Air.in :default @12:52:51.29 Air.hold @12:52:51.46 Handoff.select :blocked @12:52:51.64 Overpressure in PROCESSING -- Waste port clogged (7.6psi) @12:52:51.67 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 8psi" @12:52:51.70 BadNews.email "Waste port clogged (7.6psi)",Subject: "Overpressure in PROCESSING" @12:52:53.98 upsync @12:53:01.80 -> slots {[34,28,15,6] => { Type: :lyseHold_phyto, State: :dry}, [53,31,30] => { Type: :archive_bac, State: :missing}, 43 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50.0}, 54 => { Type: :lyseHold_phyto, State: :leaky}} @12:54:09.77 -> Syringe.psi 8.00817342279382 @12:54:25.86 -> Handoff Handoff Valve::Manifold selects blocked @12:54:31.31 -> Handoff.waste @12:54:31.34 Handoff.select :waste Handoff Valve::Manifold selects waste @12:54:38.24 -> Syringe.psi 0.573448119010038 @12:55:20.16 -> Thread[PROCESSING].resume @12:55:20.19 NameError in n -- uninitialized constant PROCESSING @12:55:24.96 -> Thread[:PROCESSING].resume # @12:55:25.04 Resuming Thread (suspended since 12:52:40) @12:55:25.08 Air syringe pumping up to 1 strokes to reach 8psi @12:55:25.11 Air stroke #1 of 1 @12:55:25.30 Air.in :compress @12:55:25.40 Air.seek :evac @12:55:25.58 0.2psi after compress @12:55:36.23 -159.9psi after compress (stable) @12:55:36.25 Air.in :default @12:55:36.37 Air.hold @12:55:36.66 at 0.3psi Opened to waste @12:55:36.67 Handoff.select :blocked @12:55:36.89 Archive syringe pumping up to 8 strokes to reach 50psi @12:55:36.90 Archive stroke #1 of 8 @12:55:37.06 Archive.in :pressurize @12:55:37.18 Archive.seek :pressurized @12:55:37.68 -> Handoff.blocked @12:55:37.72 Handoff.select :blocked Handoff Valve::Manifold selects blocked @12:55:49.63 0.9psi after pressurize @12:55:55.29 -159.9psi after pressurize (stable) @12:55:55.32 Archive.in :compress @12:55:55.43 Archive.seek :empty @12:56:08.97 1.6psi after compress @12:56:12.98 -> Air Air Syringe (AirS) at evac @12:56:16.59 -> Syringe.psi 0.332245198849137 @12:56:19.56 -159.9psi after compress (stable) @12:56:19.59 Archive.in :gulp @12:56:19.72 Archive.seek :topVent @12:56:25.89 0.9psi after gulp @12:56:31.48 -159.9psi after gulp (stable) @12:56:31.50 Archive stroke #2 of 8 @12:56:31.65 Archive.in :pressurize @12:56:31.73 Archive.seek :pressurized @12:56:38.46 -> Air.stop @12:56:38.49 Air.stop Air Syringe (AirS) at evac @12:56:43.47 -> Archive.stop @12:56:43.52 Archive.stop @12:56:43.73 Archive.in :default @12:56:43.85 Archive.hold Archive Syringe (ArS) at 355uL @12:56:44.02 Handoff.select :blocked @12:56:44.10 Slide::Error in PROCESSING -- Archive Syringe (ArS) aborted at 355uL (sensed 360uL) @12:56:44.15 Waiting to Resume from Checkpoint "Archive Syringe (ArS) aborted at 355uL (sensed 360uL)" @12:56:44.20 BadNews.email "Archive Syringe (ArS) aborted at 355uL (sensed 360uL)",Subject: "Slide::Error in PROCESSING" @12:56:46.87 upsync @13:12:58.62 -> Syringe.psi 0.384269358099516 @13:14:41.04 -> Handoff.blocked @13:14:41.07 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:14:46.42 -> Handoff.waste @13:14:46.45 Handoff.select :waste Handoff Valve::Manifold selects waste @13:14:53.01 -> Handoff.blocked @13:14:53.03 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:14:57.82 -> Handoff.waste @13:14:57.86 Handoff.select :waste Handoff Valve::Manifold selects waste @13:14:59.97 -> Handoff.blocked @13:15:00.00 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:15:09.11 -> Handoff.waste @13:15:09.16 Handoff.select :waste Handoff Valve::Manifold selects waste @13:15:11.79 -> Handoff.blocked @13:15:11.83 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:15:35.86 -> Thread[:PROCESSING].resume @13:15:35.92 Resuming Thread after Archive Syringe (ArS) aborted at 355uL (sensed 360uL) at 12:56:41 # @13:15:35.99 Archive.in :pressurize @13:15:36.13 Archive.seek :pressurized @13:15:36.88 0.8psi after pressurize @13:15:42.48 -159.9psi after pressurize (stable) @13:15:42.51 Archive.in :compress @13:15:42.62 Archive.seek :empty @13:15:56.20 1.4psi after compress @13:16:06.79 -159.9psi after compress (stable) @13:16:06.82 Archive.in :gulp @13:16:07.05 Archive.seek :topVent @13:16:13.22 0.9psi after gulp @13:16:18.81 -159.9psi after gulp (stable) @13:16:18.83 Archive stroke #3 of 8 @13:16:19.47 Archive.in :pressurize @13:16:19.55 Archive.seek :pressurized @13:16:32.00 1.0psi after pressurize @13:16:38.13 -159.9psi after pressurize (stable) @13:16:38.16 Archive.in :compress @13:16:38.27 Archive.seek :empty @13:16:51.85 1.4psi after compress @13:16:55.34 -> Archive.stop @13:16:55.39 Archive.stop Archive Syringe (ArS) empty @13:17:02.44 -159.9psi after compress (stable) @13:17:02.48 Archive.in :gulp @13:17:02.59 Archive.seek :topVent @13:17:08.76 1.0psi after gulp @13:17:08.85 -> Archive.stop @13:17:08.91 Archive.stop Archive Syringe (ArS) full @13:17:14.36 -159.9psi after gulp (stable) @13:17:14.38 Archive stroke #4 of 8 @13:17:14.52 Archive.in :pressurize @13:17:14.62 Archive.seek :pressurized @13:17:27.06 1.1psi after pressurize @13:17:33.18 -159.9psi after pressurize (stable) @13:17:33.21 Archive.in :compress @13:17:33.31 Archive.seek :empty @13:17:46.86 1.5psi after compress @13:17:57.45 -159.9psi after compress (stable) @13:17:57.48 Archive.in :gulp @13:17:57.58 Archive.seek :topVent @13:18:03.75 1.1psi after gulp @13:18:09.84 -159.9psi after gulp (stable) @13:18:09.86 Archive stroke #5 of 8 @13:18:10.01 Archive.in :pressurize @13:18:10.11 Archive.seek :pressurized @13:18:16.62 -> Thread[:PROCESSING].stop @13:18:21.89 UserAbort in n -- User Abort!! @13:18:22.56 1.1psi after pressurize @13:18:28.20 -159.9psi after pressurize (stable) @13:18:28.23 Archive.in :compress @13:18:28.93 Archive.seek :empty @13:18:42.49 1.5psi after compress @13:18:43.63 -> Archive.stop @13:18:43.65 Archive.stop Archive Syringe (ArS) empty @13:18:50.63 -> Archvie.stop @13:18:50.73 NameError in n -- uninitialized constant Archvie @13:18:53.10 -159.9psi after compress (stable) @13:18:53.13 Archive.in :gulp @13:18:53.26 Archive.seek :topVent @13:18:54.24 -> Archive.stop @13:18:54.27 Archive.stop @13:18:54.51 Archive.in :default @13:18:54.62 Archive.hold Archive Syringe (ArS) at 280uL @13:18:54.80 Handoff.select :blocked @13:18:54.84 Slide::Error in PROCESSING -- Archive Syringe (ArS) aborted at 279uL (sensed 282uL) @13:18:54.90 BadNews.email "Archive Syringe (ArS) aborted at 279uL (sensed 282uL)",Subject: "Slide::Error in PROCESSING" @13:18:54.94 Waiting to Resume from Checkpoint "Archive Syringe (ArS) aborted at 279uL (sensed 282uL)" @13:18:57.05 upsync @13:19:13.45 -> Handoff.waste @13:19:13.48 Handoff.select :waste Handoff Valve::Manifold selects waste @13:19:42.74 -> ArS.empty @13:19:42.79 Archive.seek :empty Archive Syringe (ArS) empty @13:20:02.04 -> ArS.jog -50 @13:20:02.07 Archive.jog -50 Archive Syringe (ArS) at -12uL @13:20:18.47 -> ArS.jog -50 @13:20:18.52 Archive.jog -50 Archive Syringe (ArS) at -24uL @13:20:26.55 -> ArS.full @13:20:26.59 Archive.seek :full Archive Syringe (ArS) full @13:21:23.98 -> Handoff.blocked @13:21:24.00 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:21:28.91 -> Thread[:PROCESSING].stop @13:21:35.52 UserAbort in n -- User Abort!! @13:22:11.36 -> ArS.empty; ArS.jog -100; delay 4; Syringe.psi @13:22:11.40 Archive.seek :empty @13:22:26.70 Archive.jog -100 @13:22:27.37 delay 4 seconds 14.1659420904315 @13:23:54.91 -> Syringe.psi 13.9531159844072 @13:26:35.38 -> ArS.full;ArS.empty; ArS.jog -100; delay 4; Syringe.psi @13:26:35.41 Archive.seek :full @13:26:50.91 Archive.seek :empty @13:27:06.18 Archive.jog -100 @13:27:06.84 delay 4 seconds 26.4436436735248 @13:27:29.14 -> ArS.full;ArS.empty; ArS.jog -100; delay 4; Syringe.psi @13:27:29.19 Archive.seek :full @13:27:44.64 Archive.seek :empty @13:27:59.91 Archive.jog -100 @13:28:00.59 delay 4 seconds 38.6125674690945 @13:28:20.65 -> ArS.full;ArS.empty; ArS.jog -100; delay 4; Syringe.psi @13:28:20.70 Archive.seek :full @13:28:36.16 Archive.seek :empty @13:28:51.41 Archive.jog -100 @13:28:52.12 delay 4 seconds 49.9443752476351 @13:29:06.54 -> Handoff.waste @13:29:06.56 Handoff.select :waste Handoff Valve::Manifold selects waste @13:29:18.86 -> AirS.fvent @13:29:18.92 Air.seek :fvent Air Syringe (AirS) at fVent @13:29:40.66 -> ArS.full @13:29:40.72 Archive.seek :full Archive Syringe (ArS) full @13:30:31.42 -> Air.evac @13:30:31.47 Air.seek :evac Air Syringe (AirS) at evac @13:30:46.06 -> ArS.empty;AirS.jog -100 @13:30:46.13 Archive.seek :empty @13:31:01.42 Air.jog -100 Air Syringe (AirS) at 113uL @13:31:07.95 -> ArS.jog -100 @13:31:07.98 Archive.jog -100 Archive Syringe (ArS) at -24uL @13:31:16.74 -> ArS.jog -50 @13:31:16.78 Archive.jog -50 Archive Syringe (ArS) at -36uL @13:31:27.89 -> ArS.status.position -8380 @13:31:34.53 -> ArS.jog -50 @13:31:34.57 Archive.jog -50 Archive Syringe (ArS) at -48uL @13:31:38.55 -> ArS.jog -50 @13:31:38.58 Archive.jog -50 Archive Syringe (ArS) at -60uL @13:31:48.93 -> ArS.jog -50 @13:31:48.96 Archive.jog -50 Archive Syringe (ArS) at -72uL @13:31:59.69 -> ArS.status.position -8530 @13:34:15.56 -> Air.fvent @13:34:15.59 Air.seek :fvent Air Syringe (AirS) at fVent @13:34:24.58 -> Air.clear;ArS.clear @13:34:24.64 Air.seek :clear @13:34:41.91 Archive.seek :clear Archive Syringe (ArS) clear @13:35:06.40 -> Cartridge.unlock @13:35:06.47 Gate.power :heat,:OFF @13:35:06.49 Gate.power :cartridge,:OFF @13:35:06.62 PinPort.in :unlocking @13:35:06.70 PinPort.seek :clear @13:35:07.20 Carousel.in :default @13:35:07.35 Carousel.home.seek 43 @13:35:07.87 Lysis2.in :unlocking @13:35:07.95 Lysis2.seek :clear @13:35:12.53 Lysis1.in :unlocking @13:35:12.67 Lysis1.seek :clear @13:35:12.79 Plunge.in :unlocking @13:35:12.90 Plunge.seek :clear @13:35:16.87 -> exit @13:35:16.91 Session Ended @13:35:25.82 Session Begun @13:35:32.59 -> MainThread.exit # @13:35:33.37 Handoff.select :close @13:35:33.61 Session Ended @13:35:43.45PDT16-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:35:45.12 >BREAK< ESP3 Gateway v1.43 for core bus @13:35:45.15 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:35:48.56 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @13:35:49.22 Sea pressure (Abs) tare is 6% of full scale @13:35:52.76 Polling Can every 2:00 @13:35:52.82 Waiting for espclients... @13:35:53.86 Session Begun @13:36:27.15 -> ESP.ready! @13:36:27.34 Gate.power :sampler,:OFF,limit to 3500mA @13:36:27.37 Gate.power :skimmer,:OFF,limit to 3500mA @13:36:27.40 Gate.power :analytic1,:ON,limit to 4000mA @13:36:28.15 Plunge.configure :default @13:36:28.25 Plunge.hold @13:36:28.35 Plunge.configure :default @13:36:28.51 SamplePump.configure :default @13:36:28.69 Toroid.configure :default @13:36:28.78 Toroid.hold @13:36:28.89 Toroid.configure :default @13:36:29.05 Carousel.configure :default @13:36:29.15 Carousel.hold @13:36:29.26 Carousel.configure :default @13:36:29.69 Abs.configure :default @13:36:29.85 PhaseSeparator.configure :default @13:36:30.02 Archive.configure :default @13:36:30.12 Archive.hold @13:36:30.25 Archive.configure :default @13:36:30.42 Air.configure :default @13:36:30.51 Air.hold @13:36:30.62 Air.configure :default @13:36:30.76 Lysis1.configure :default @13:36:30.84 Lysis1.hold @13:36:30.94 Lysis1.configure :default @13:36:31.09 Lysis2.configure :default @13:36:31.43 Lysis2.hold @13:36:31.54 Lysis2.configure :default @13:36:31.70 PinPort.configure :default @13:36:31.79 PinPort.hold @13:36:31.89 PinPort.configure :default @13:36:31.98 All elf controllers running firmware version 4.92 @13:36:32.93 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:36:32.96 AnalyticModule::Unknown in n -- SPR @13:36:33.79 Toroid.seek :clear @13:36:34.01 Carousel.in :default @13:36:34.18 Carousel.home.seek 43 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 50ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 43 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:36:42.64 -> ESP.reready! @13:36:42.81 Plunge.in :default @13:36:42.88 Toroid.in :default @13:36:43.00 Carousel.in :default @13:36:43.65 Archive.in :default @13:36:43.75 Plunge.home.jog 0 @13:36:43.83 Air.in :default @13:36:43.90 Lysis1.in :default @13:36:43.94 Lysis2.in :default @13:36:44.00 Toroid.home.jog 0 @13:36:44.11 PinPort.in :default @13:36:44.20 Carousel.home.jog 0 @13:36:44.44 Archive.home.jog 0 @13:36:44.68 Air.home.jog 0 @13:36:44.76 Lysis2.home.jog 0 @13:36:44.88 Lysis1.home.jog 0 @13:36:44.96 PinPort.home.jog 0 @13:36:45.81 Toroid.in :homing @13:36:45.87 Plunge.in :homing @13:36:46.12 Toroid.jog 635 @13:36:46.14 Plunge.jog 165 @13:36:46.45 Lysis2.in :homing @13:36:46.51 Lysis1.in :homing @13:36:46.56 Archive.in :homing @13:36:46.60 PinPort.in :homing @13:36:46.70 Air.in :homing @13:36:46.86 Lysis2.jog 1408 @13:36:46.92 Lysis1.jog 1395 @13:36:46.99 PinPort.jog -106 @13:36:47.04 Archive.jog 1387 @13:36:47.12 Air.jog 1370 @13:36:47.52 Plunge.setRawPosition! 50 @13:36:47.61 Plunge.seek :home @13:36:47.75 PinPort.setRawPosition! 0 @13:36:47.84 PinPort.seek :home @13:36:47.97 Plunge.in :default @13:36:48.11 PinPort.in :default @13:36:48.32 Lysis2.setRawPosition! 50 @13:36:48.41 Lysis2.seek :home @13:36:48.56 Lysis1.setRawPosition! 50 @13:36:48.61 Air.setRawPosition! 50 @13:36:48.65 Archive.setRawPosition! 50 @13:36:48.70 Toroid.setRawPosition! 0 @13:36:48.94 Lysis1.seek :home @13:36:48.98 Air.seek :home @13:36:49.03 Archive.seek :home @13:36:49.10 Toroid.seek :home @13:36:49.50 Lysis2.in :default @13:36:49.54 Lysis1.in :default @13:36:49.59 Toroid.in :default @13:36:49.82 Archive.in :default @13:36:49.85 Air.in :default @13:36:50.27 Toroid.seek :clear @13:36:52.01 Carousel.in :default @13:36:52.19 Carousel.home.seek 43 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 50ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 43 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:37:27.09 -> Cartridge.lock @13:37:27.27 Lysis1.in :fast @13:37:27.35 Lysis1.seek :locked @13:37:30.40 Plunge.seek :locked @13:37:30.70 Air.in :fast @13:37:30.78 Air.seek :locked @13:37:31.79 Carousel.reconfigure :locked @13:37:31.98 Powering cartridge bus @13:37:34.64 Gate.setIR 1750,5000,0,6 @13:37:34.77 Gate.setIR 0,5000,0,6 @13:37:34.86 PuckHeater.configure :default @13:37:34.95 Gate.power :heat,:ON,limit to 5000mA @13:37:35.69 Mounted lysis Cartridge board#Q05075 @13:37:35.74 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @13:38:11.65 -> SC Storage Carousel (SC) at 43 @13:38:17.08 -> slot 43=>nil {43 => nil} @13:38:26.60 -> slot 43=>[:dry,:lyseHold_bac] {43 => { Type: :lyseHold_bac, State: :dry}} @13:38:37.14 -> fire lyseHold_bac # @13:38:38.14 Toroid.seek :clear @13:38:38.36 Carousel.in :default @13:38:38.51 Carousel.home.seek 43 @13:38:38.91 Carousel.reconfigure :locked @13:38:39.16 Gate.setIR 1750,5000,0,6 @13:38:39.29 Gate.setIR 0,5000,0,6 @13:38:39.39 PuckHeater.configure :default @13:38:39.49 Gate.power :heat,:ON,limit to 5000mA @13:38:40.20 Mounted lysis Cartridge board#Q05075 @13:38:40.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:38:40.34 Selecting Cartridge 43 @13:38:40.73 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling lyseHold_bac cartridge in slot 43" @13:38:41.19 Preparing to filter cartridge 43 @13:38:41.55 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @13:38:43.35 upsync @13:38:48.26 Intake.close @13:38:48.31 Exhaust.close @13:38:48.43 Bypass.close @13:38:48.83 Scale::Skew::CannotTare in FILTERING -- Intake Head Pressure sensor @31.3% scale out of usable range @13:38:48.86 BadNews.email "Intake Head Pressure sensor @31.3% scale out of usable range",Subject: "Scale::Skew::CannotTare in FILTERING" @13:38:48.91 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi" @13:38:51.04 upsync @13:40:25.71 -> Cartridge.sampler.source=nil @13:40:32.22 -> Cartridge.lock @13:40:32.27 Gate.power :heat,:OFF @13:40:32.30 Gate.power :cartridge,:OFF @13:40:33.88 Powering cartridge bus @13:40:36.54 Gate.setIR 1750,5000,0,6 @13:40:36.68 Gate.setIR 0,5000,0,6 @13:40:36.77 PuckHeater.configure :default @13:40:36.86 Gate.power :heat,:ON,limit to 5000mA @13:40:37.57 Mounted lysis Cartridge board#Q05075 @13:40:37.63 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_bac @13:40:41.41 -> Cartridge.sampler.source=nil @13:40:49.33 -> fire lyseHold_bac @13:40:49.52 Selecting Cartridge 43 # @13:40:50.00 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling lyseHold_bac cartridge in slot 43" @13:40:50.49 Preparing to filter cartridge 43 @13:40:51.26 Air.in :default @13:40:51.58 Air.seek :sealed @13:40:51.72 Priming sample loop w/150ml, bypass w/50ml @13:40:51.76 SamplePump.setPosition! 0ml @13:40:51.80 Cmd::Error in detached from n -- Cannot process while in PRIMING state @13:40:51.92 Waiting to Resume from Checkpoint "Selecting Cartridge 43" @13:40:51.98 Bypass.open @13:40:52.27 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_bac cartridge in slot 43" @13:40:52.99 upsync @13:40:53.77 Intake.open @13:40:54.48 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n" @13:40:55.10 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:40:55.67 upsync @13:40:57.57 upsync @13:40:57.79 Vacuum tare is 52% of full scale at 22C (drifted 1%) @13:40:57.99 Abs.in :default @13:40:58.10 Abs.hold @13:40:58.40 SamplePump.in :start @13:40:58.50 SamplePump.seek 50ml,3:50 @13:40:59.10 SamplePump.configure :prime @13:41:59.89 Abs.stop @13:41:59.98 SamplePump.setPosition! 0ml @13:42:00.06 Exhaust.open @13:42:01.51 Bypass.close @13:42:03.04 Abs.in :default @13:42:03.13 Abs.hold @13:42:03.46 SamplePump.in :start @13:42:03.56 SamplePump.seek 150ml,10:30 @13:42:04.15 SamplePump.configure :prime @13:45:06.89 Abs.stop @13:45:07.10 Bypass.open @13:45:07.83 Intake.close @13:45:08.59 Toroid.in :hold @13:45:08.69 Toroid.seek :crack @13:45:10.26 Toroid.seek :cartridge @13:45:11.40 Toroid.in :default @13:45:11.50 Toroid.seek :clear @13:45:13.57 Pressurizing cartridge to 26psi above 15.6psia @13:45:13.59 SamplePump.setPosition! 0ml @13:45:13.69 Intake.open @13:45:14.37 Bypass.close @13:45:15.07 Exhaust.close @13:45:15.84 Abs.in :default @13:45:15.93 Abs.hold @13:45:16.21 SamplePump.in :charge @13:45:16.38 SamplePump.seek 2.3ml,42 seconds @13:45:30.44 Abs.stop @13:45:30.55 Intake.close @13:45:31.31 Pressurized cartridge with 0.885ml to 28.5psi @13:45:31.33 Isolated @38.4psia (22.8psi) @13:45:31.37 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @13:45:31.44 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:45:36.06 Cartridge pressure fell 2% to 37.6psia @13:45:36.22 Sampling 1000.0ml @13:45:36.24 SamplePump.setPosition! 0ml @13:45:36.34 Exhaust.open @13:45:36.98 Intake.open @13:45:37.84 Abs.in :default @13:45:37.91 Abs.hold @13:45:38.30 SamplePump.in :start @13:45:38.40 SamplePump.seek 1000ml,1:23:50 @13:45:38.99 SamplePump.configure :sample @13:45:41.40 Cartridge 43 filtering 2/1000ml at 0.82ml/s with 13.9psi vacuum 4.9m deep (21.9psia) @13:46:41.45 Cartridge 43 filtering 39/1000ml at 0.63ml/s with 18.9psi vacuum 5.4m deep (22.6psia) @13:47:41.46 Cartridge 43 filtering 77/1000ml at 0.62ml/s with 21.4psi vacuum @13:48:41.45 Cartridge 43 filtering 114/1000ml at 0.62ml/s with 19.6psi vacuum @13:49:41.45 Cartridge 43 filtering 151/1000ml at 0.62ml/s with 22.2psi vacuum @13:50:41.45 Cartridge 43 filtering 188/1000ml at 0.61ml/s with 14.6psi vacuum @13:51:05.86 -> Cmds.top @13:51:05.89 NameError in n -- uninitialized constant Cmds @13:51:08.28 -> Cmd.stop @13:51:08.31 Abs.stop @13:51:08.52 Intake.close @13:51:08.59 Exhaust.close @13:51:08.87 Toroid.in :hold @13:51:08.94 Toroid.seek :bypass @13:51:10.59 Toroid.in :default @13:51:10.66 Toroid.seek :clear @13:51:13.17 Gate.power :heat,:OFF @13:51:13.19 Gate.power :cartridge,:OFF @13:51:13.35 Carousel.in :default @13:51:13.53 GoodNews.email "After filtering 204.5ml",Subject: "Processing cartridge in slot 43" @13:51:13.71 Cartridge::Error in detached from n#2 -- Not Mounted @13:51:13.77 Waiting to Resume from Checkpoint "Selecting Cartridge 43" @13:51:13.85 Carousel.home.seek 43 @13:51:14.37 Plunge.seek :locked @13:51:14.81 Air.in :unlocking @13:51:15.05 Air.seek :clear @13:51:15.14 Lysis1.in :unlocking @13:51:15.45 Lysis1.seek :clear @13:51:15.92 BadNews.email "Not Mounted",Subject: "Cartridge::Error in detached from n#2" @13:51:16.83 upsync @13:51:17.14 Gate.power :analytic1,:OFF @13:51:17.18 Gate.power :motor,:OFF @13:51:17.26 Gate.power :core,:OFF @13:51:17.31 Gate.power :main,:OFF @13:51:17.40 Safely stopped and ready to power off :STOPPED @13:51:19.13 upsync @13:51:29.56 -> slot 43=>[:dry,:lyseHold_phyto] {43 => { Type: :lyseHold_phyto, State: :dry}} @13:52:00.03 -> PhytoSampler.bypassVolume=10; PhytoSampler.primeVolume=10 10 @13:52:08.12 -> fire lyseHold_phyto # @13:52:08.36 Gate.power :sampler,:OFF,limit to 3500mA @13:52:08.41 Gate.power :skimmer,:OFF,limit to 3500mA @13:52:08.45 Gate.power :main,:ON,limit to 5000mA @13:52:08.50 Gate.power :analytic1,:ON,limit to 4000mA @13:52:08.63 Powering core bus @13:52:11.42 Plunge.configure :default @13:52:11.54 SamplePump.configure :default @13:52:11.65 Toroid.configure :default @13:52:11.75 Carousel.configure :default @13:52:11.87 Abs.configure :default @13:52:11.98 PhaseSeparator.configure :default @13:52:12.11 Archive.configure :default @13:52:12.23 Air.configure :default @13:52:12.32 Lysis1.configure :default @13:52:12.68 Lysis2.configure :default @13:52:12.79 PinPort.configure :default @13:52:12.87 All elf controllers running firmware version 4.92 @13:52:12.91 Gate.power :motor,:ON,limit to 5000mA @13:52:15.89 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:52:15.91 AnalyticModule::Unknown in detached from n#3 -- SPR @13:52:16.37 Plunge.home.jog 0 @13:52:16.93 Lysis2.home.jog 0 @13:52:16.99 Lysis1.home.jog 0 @13:52:17.06 Air.home.jog 0 @13:52:17.12 Archive.home.jog 0 @13:52:17.18 Carousel.home.jog 0 @13:52:17.23 Toroid.home.jog 0 @13:52:17.72 PinPort.home.jog 0 @13:52:18.72 Plunge.in :homing @13:52:19.02 Plunge.jog 1614 @13:52:19.25 Lysis2.in :homing @13:52:19.30 Lysis1.in :homing @13:52:19.34 Archive.in :homing @13:52:19.39 PinPort.in :homing @13:52:19.46 Air.in :homing @13:52:19.51 Toroid.in :homing @13:52:19.79 Lysis2.jog 1406 @13:52:19.84 Lysis1.jog 1394 @13:52:20.17 Archive.jog 1402 @13:52:20.22 PinPort.jog -107 @13:52:20.27 Air.jog 1371 @13:52:20.33 Toroid.jog 617 @13:52:20.73 PinPort.setRawPosition! 0 @13:52:20.76 Plunge.setRawPosition! 50 @13:52:20.89 PinPort.seek :home @13:52:21.01 Plunge.seek :home @13:52:21.21 PinPort.in :default @13:52:21.35 Lysis2.setRawPosition! 50 @13:52:21.44 Lysis1.setRawPosition! 50 @13:52:21.51 Lysis2.seek :home @13:52:21.64 Lysis1.seek :home @13:52:21.84 Air.setRawPosition! 50 @13:52:21.89 Archive.setRawPosition! 50 @13:52:22.00 Plunge.in :default @13:52:22.13 Air.seek :home @13:52:22.31 Archive.seek :home @13:52:22.47 Lysis1.in :default @13:52:22.49 Lysis2.in :default @13:52:22.74 Archive.in :default @13:52:22.77 Toroid.setRawPosition! 0 @13:52:22.83 Air.in :default @13:52:23.03 Toroid.seek :home @13:52:23.19 Toroid.in :default @13:52:23.34 Toroid.seek :clear @13:52:25.06 Carousel.in :default @13:52:25.21 Carousel.home.seek 43 @13:52:25.49 Selecting Cartridge 43 @13:52:25.80 Carousel.seek 43 @13:52:26.15 Lysis1.in :fast @13:52:26.24 Lysis1.seek :locked @13:52:28.90 Plunge.seek :locked @13:52:29.10 Air.in :fast @13:52:29.19 Air.seek :locked @13:52:30.19 Carousel.reconfigure :locked @13:52:30.38 Powering cartridge bus @13:52:33.05 Gate.setIR 1750,5000,0,6 @13:52:33.17 Gate.setIR 0,5000,0,6 @13:52:33.27 PuckHeater.configure :default @13:52:33.78 Gate.power :heat,:ON,limit to 5000mA @13:52:34.58 Mounted lysis Cartridge board#Q05075 @13:52:34.64 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:52:34.69 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 43" @13:52:35.15 Preparing to filter cartridge 43 @13:52:35.46 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @13:52:37.39 upsync @13:52:42.20 Scale::Skew::CannotTare in FILTERING -- Intake Head Pressure sensor @31.3% scale out of usable range @13:52:42.22 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi" @13:52:42.27 BadNews.email "Intake Head Pressure sensor @31.3% scale out of usable range",Subject: "Scale::Skew::CannotTare in FILTERING" @13:52:44.32 upsync @13:53:37.42 -> Cartridge.sampler.source=nil @13:53:40.19 -> fire lyseHold_phyto @13:53:40.40 Selecting Cartridge 43 # @13:53:40.87 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 43" @13:53:41.36 Preparing to filter cartridge 43 @13:53:42.88 Air.in :default @13:53:43.14 Air.seek :sealed @13:53:43.29 Priming sample loop w/10ml, bypass w/10ml @13:53:43.31 Cmd::Error in detached from n#3 -- Cannot process while in PRIMING state @13:53:43.37 SamplePump.setPosition! 0ml @13:53:43.41 Waiting to Resume from Checkpoint "Selecting Cartridge 43" @13:53:43.67 Bypass.open @13:53:43.72 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 43" @13:53:44.66 upsync @13:53:45.52 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n#3" @13:53:45.88 Intake.open @13:53:46.58 upsync @13:53:46.98 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:53:48.14 upsync @13:53:49.62 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:53:49.82 Abs.in :default @13:53:49.91 Abs.hold @13:53:50.24 SamplePump.in :start @13:53:50.35 SamplePump.seek 10ml,1:10 @13:53:50.93 SamplePump.configure :prime @13:54:02.94 Abs.stop @13:54:03.04 SamplePump.setPosition! 0ml @13:54:03.13 Exhaust.open @13:54:04.47 Bypass.close @13:54:06.05 Abs.hold @13:54:06.45 SamplePump.in :start @13:54:06.55 SamplePump.seek 10ml,1:10 @13:54:07.14 SamplePump.configure :prime @13:54:19.14 Abs.stop @13:54:19.25 Bypass.open @13:54:19.94 Intake.close @13:54:20.73 Toroid.in :hold @13:54:20.81 Toroid.seek :crack @13:54:22.39 Toroid.seek :cartridge @13:54:23.54 Toroid.in :default @13:54:23.63 Toroid.seek :clear @13:54:25.77 Pressurizing cartridge to 26psi above 15.6psia @13:54:25.79 SamplePump.setPosition! 0ml @13:54:25.88 Intake.open @13:54:26.59 Bypass.close @13:54:27.27 Exhaust.close @13:54:28.05 Abs.in :default @13:54:28.18 Abs.hold @13:54:28.44 SamplePump.in :charge @13:54:28.54 SamplePump.seek 2.3ml,42 seconds @13:54:38.65 Abs.stop @13:54:38.76 Intake.close @13:54:39.54 Pressurized cartridge with 0.454ml to 26.8psi @13:54:39.56 Isolated @39.9psia (24.3psi) @13:54:39.60 Verifying cartridge holds at least 0.65..0.85 of 39.9psia @13:54:39.66 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:54:44.28 Cartridge pressure fell 2% to 39.0psia @13:54:44.42 Sampling 50.0ml @13:54:44.44 SamplePump.setPosition! 0ml @13:54:44.54 Exhaust.open @13:54:45.20 Intake.open @13:54:46.08 Abs.in :default @13:54:46.19 Abs.hold @13:54:46.55 SamplePump.in :start @13:54:46.65 SamplePump.seek 50ml,4:40 @13:54:47.24 SamplePump.configure :sample @13:54:49.64 Cartridge 43 filtering 2/50ml at 0.54ml/s with 20.5psi vacuum @13:55:49.70 Cartridge 43 filtering 39/50ml at 0.62ml/s with 33.9psi vacuum @13:56:08.21 Abs.stop @13:56:08.40 Intake.close @13:56:08.47 Exhaust.close @13:56:08.74 Toroid.in :hold @13:56:08.82 Toroid.seek :bypass @13:56:10.48 Toroid.in :default @13:56:10.57 Toroid.seek :clear @13:56:12.78 Sampled 50.0ml @13:56:13.24 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 43" @13:56:13.75 Handoff.select :waste @13:56:13.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:56:16.10 upsync @13:56:20.62 Syringe tare is 52% of full scale at 22C (drifted 1%) @13:56:20.66 PinPort.in :locking @13:56:20.76 PinPort.seek :locked @13:56:21.28 Archive.in :fast @13:56:21.39 Lysis2.in :fast @13:56:21.46 Lysis1.seek 1350uL @13:56:21.51 Archive.seek :full @13:56:21.70 Lysis2.seek :empty @13:56:29.80 Plunge.seek :reagent @13:56:30.74 Evacuating puck with filter bubble point of 50psi @13:56:30.77 PinPort.seek :locked @13:56:30.91 Handoff.select :waste @13:56:30.95 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:56:37.56 Air syringe pumping up to 1 strokes to reach 8psi @13:56:37.57 Air stroke #1 of 1 @13:56:37.71 Air.in :pressurize @13:56:37.79 Air.seek :pressurized @13:56:43.45 3.8psi after pressurize @13:56:43.48 Air.in :compress @13:56:43.58 Air.seek :evac @13:56:46.62 2.6psi after compress @13:56:57.21 -159.8psi after compress (stable) @13:56:57.23 Air.in :default @13:56:57.34 Air.hold @13:56:57.66 at 0.9psi Opened to waste @13:56:57.68 Handoff.select :blocked @13:56:57.86 Archive syringe pumping up to 8 strokes to reach 50psi @13:56:57.87 Archive stroke #1 of 8 @13:56:58.00 Archive.in :pressurize @13:56:58.12 Archive.seek :pressurized @13:57:10.59 1.4psi after pressurize @13:57:16.20 -159.8psi after pressurize (stable) @13:57:16.23 Archive.in :compress @13:57:16.33 Archive.seek :empty @13:57:32.10 2.0psi after compress @13:57:42.70 -159.8psi after compress (stable) @13:57:42.72 Archive.in :gulp @13:57:42.82 Archive.seek :topVent @13:57:49.60 1.3psi after gulp @13:57:55.20 -159.8psi after gulp (stable) @13:57:55.21 Archive stroke #2 of 8 @13:57:55.35 Archive.in :pressurize @13:57:55.43 Archive.seek :pressurized @13:57:57.85 -> Handoff.blocked @13:57:57.87 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:58:07.90 1.6psi after pressurize @13:58:13.99 -159.8psi after pressurize (stable) @13:58:14.02 Archive.in :compress @13:58:14.15 Archive.seek :empty @13:58:29.90 1.3psi after compress @13:58:40.49 -159.8psi after compress (stable) @13:58:40.52 Archive.in :gulp @13:58:40.62 Archive.seek :topVent @13:58:46.96 0.9psi after gulp @13:58:53.05 -159.8psi after gulp (stable) @13:58:53.07 Archive stroke #3 of 8 @13:58:53.21 Archive.in :pressurize @13:58:53.42 Archive.seek :pressurized @13:58:53.45 -> Handoff.blocked @13:58:53.53 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:59:05.93 0.9psi after pressurize @13:59:11.52 -159.9psi after pressurize (stable) @13:59:11.55 Archive.in :compress @13:59:11.67 Archive.seek :empty @13:59:27.43 1.4psi after compress @13:59:34.19 -> Handoff.waste @13:59:34.23 Handoff.select :waste Handoff Valve::Manifold selects waste @13:59:38.02 -159.9psi after compress (stable) @13:59:38.07 Archive.in :gulp @13:59:38.25 Archive.seek :topVent @13:59:38.84 -> Handoff.blocked @13:59:38.87 Handoff.select :blocked Handoff Valve::Manifold selects blocked @13:59:44.60 0.9psi after gulp @13:59:50.19 -159.8psi after gulp (stable) @13:59:50.21 Archive stroke #4 of 8 @13:59:50.34 Archive.in :pressurize @13:59:50.86 Archive.seek :pressurized @14:00:03.44 0.9psi after pressurize @14:00:09.53 -159.8psi after pressurize (stable) @14:00:09.56 Archive.in :compress @14:00:09.66 Archive.seek :empty @14:00:25.41 1.6psi after compress @14:00:36.00 -159.8psi after compress (stable) @14:00:36.03 Archive.in :gulp @14:00:36.18 Archive.seek :topVent @14:00:42.53 1.0psi after gulp @14:00:45.38 -> Archive.stop @14:00:45.42 Archive.stop Archive Syringe (ArS) full @14:00:48.15 -159.8psi after gulp (stable) @14:00:48.17 Archive stroke #5 of 8 @14:00:48.31 Archive.in :pressurize @14:00:48.40 Archive.seek :pressurized @14:00:52.32 -> Archive.stop @14:00:52.34 Archive.stop @14:00:52.58 Archive.in :default @14:00:52.72 Archive.hold Archive Syringe (ArS) at 1284uL @14:00:52.89 Handoff.select :blocked @14:00:52.94 Slide::Error in PROCESSING -- Archive Syringe (ArS) aborted at 1284uL (sensed 1289uL) @14:00:53.53 Waiting to Resume from Checkpoint "Archive Syringe (ArS) aborted at 1284uL (sensed 1289uL)" @14:00:53.57 BadNews.email "Archive Syringe (ArS) aborted at 1284uL (sensed 1289uL)",Subject: "Slide::Error in PROCESSING" @14:00:56.39 upsync @14:01:04.58 -> Air.fvent @14:01:04.62 Air.seek :fvent Air Syringe (AirS) at fVent @14:01:14.30 -> Handoff.waste @14:01:14.34 Handoff.select :waste Handoff Valve::Manifold selects waste @14:01:18.02 -> Cartridge.unlock @14:01:18.12 Gate.power :heat,:OFF @14:01:18.14 Gate.power :cartridge,:OFF @14:01:18.28 PinPort.in :unlocking @14:01:18.37 PinPort.seek :clear @14:01:18.88 Carousel.in :default @14:01:19.02 Carousel.home.seek 43 @14:01:19.42 Archive.in :unlocking @14:01:19.59 Archive.seek :clear @14:01:19.72 Air.in :unlocking @14:01:19.86 Air.seek :clear @14:01:19.99 Lysis2.in :unlocking @14:01:20.10 Lysis2.seek :clear @14:01:24.71 Lysis1.in :unlocking @14:01:24.86 Lysis1.seek :clear @14:01:24.94 Plunge.in :unlocking @14:01:25.08 Plunge.seek :clear @14:02:22.50 -> Cartridge.lock @14:02:22.69 Lysis1.in :fast @14:02:22.77 Lysis1.seek :locked @14:02:25.40 Plunge.in :default @14:02:25.48 Plunge.seek :locked @14:02:25.69 Air.in :fast @14:02:25.77 Air.seek :locked @14:02:26.78 Carousel.reconfigure :locked @14:02:26.96 Powering cartridge bus @14:02:29.66 Gate.setIR 1750,5000,0,6 @14:02:29.79 Gate.setIR 0,5000,0,6 @14:02:29.89 PuckHeater.configure :default @14:02:29.98 Gate.power :heat,:ON,limit to 5000mA @14:02:30.68 Mounted lysis Cartridge board#Q05075 @14:02:30.73 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @14:03:05.28 -> Lysis1.to 1300 @14:03:05.33 Lysis1.in :default @14:03:05.42 Lysis1.seek 1300uL Lysis Syringe #1 (LyS1) at 1300uL @14:03:16.10 -> PP.connect @14:03:16.19 PinPort.in :locking @14:03:16.74 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:03:35.58 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2235 => ["cartridge",:end,:insert,:inserted,:locked]} @14:03:55.72 -> Handoff.waste @14:03:55.75 Handoff.select :waste Handoff Valve::Manifold selects waste @14:04:18.27 -> Sampler.psi -0.0611429779145283 @14:04:58.68 -> PP.cartridge @14:04:58.71 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:05:11.02 -> LyS2.empty @14:05:11.06 Lysis2.in :default @14:05:11.15 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @14:05:39.32 -> LyS2.full @14:05:39.38 Lysis2.seek :full Lysis Syringe #2 (LyS2) locked @14:06:02.17 -> PP.jog -50 @14:06:02.23 PinPort.in :default @14:06:02.29 PinPort.jog -50 Handoff Pin Port (PP) at cartridge - 50 counts @14:06:20.42 -> LyS2.empty @14:06:20.48 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @14:06:59.41 -> MainThread.exit # @14:06:59.61 Handoff.select :close @14:06:59.84 Session Ended @14:08:53.08PDT16-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @14:08:54.72 >BREAK< ESP3 Gateway v1.43 for core bus @14:08:54.75 >BREAK< ESP3 Gateway v1.43 for cartridge bus @14:08:58.30 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @14:08:59.02 Sea pressure (Abs) tare is 6% of full scale @14:09:02.65 Polling Can every 2:00 @14:09:02.69 Waiting for espclients... @14:09:03.75 Session Begun @14:09:09.37 -> Cartridge.activate @14:09:09.59 Gate.setIR 1750,5000,0,6 @14:09:09.72 Gate.setIR 0,5000,0,6 @14:09:09.81 PuckHeater.configure :default @14:09:09.90 Gate.power :heat,:ON,limit to 5000mA @14:09:10.65 Mounted lysis Cartridge board#Q05075 @14:09:10.70 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @14:09:46.91 -> PhytoSampler.lyseHold @14:09:46.93 Handoff.select :waste @14:09:47.14 Gate.power :sampler,:OFF,limit to 3500mA @14:09:47.16 Gate.power :skimmer,:OFF,limit to 3500mA @14:09:47.20 Gate.power :analytic1,:ON,limit to 4000mA @14:09:47.90 Plunge.configure :default @14:09:47.99 Plunge.hold @14:09:48.16 Plunge.configure :default @14:09:48.35 SamplePump.configure :default @14:09:48.55 Toroid.configure :default @14:09:48.65 Toroid.hold @14:09:48.75 Toroid.configure :default @14:09:48.93 Carousel.configure :default @14:09:49.02 Carousel.hold @14:09:49.12 Carousel.configure :default @14:09:49.56 Abs.configure :default @14:09:49.73 PhaseSeparator.configure :default @14:09:49.90 Archive.configure :default @14:09:49.99 Archive.hold @14:09:50.14 Archive.configure :default @14:09:50.31 Air.configure :default @14:09:50.40 Air.hold @14:09:50.50 Air.configure :default @14:09:50.65 Lysis1.configure :default @14:09:50.74 Lysis1.hold @14:09:50.86 Lysis1.configure :default @14:09:51.27 Lysis2.configure :default @14:09:51.37 Lysis2.hold @14:09:51.47 Lysis2.configure :default @14:09:51.65 PinPort.configure :default @14:09:51.74 PinPort.hold @14:09:51.85 PinPort.configure :default @14:09:51.94 WARNING: Multiple controller firmware versions in use [:ambient0, :valve1, :carousel0, :carousel1, :syringe0, :sampler0, :syringe1, :sampler1, :valve0]=>4.92, [:Cartridge]=>4.44 @14:09:52.77 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @14:09:52.80 AnalyticModule::Unknown in n -- SPR @14:09:52.97 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:09:59.60 Syringe tare is 52% of full scale at 22C (drifted 1%) @14:09:59.65 PinPort.in :locking @14:10:00.15 PinPort.seek :locked @14:10:00.39 Archive.in :fast @14:10:00.45 Lysis2.in :fast @14:10:00.54 Lysis1.in :fast @14:10:00.66 Archive.seek :full @14:10:00.81 Lysis2.seek :empty @14:10:00.87 Lysis1.seek 1350uL @14:10:02.71 Plunge.seek :reagent @14:10:03.63 Evacuating puck with filter bubble point of 50psi @14:10:03.66 PinPort.seek :locked @14:10:03.81 Handoff.select :waste @14:10:03.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:10:10.46 Air syringe pumping up to 1 strokes to reach 8psi @14:10:10.47 Air stroke #1 of 1 @14:10:10.61 Air.in :pressurize @14:10:10.69 Air.seek :pressurized @14:10:17.21 2.8psi after pressurize @14:10:17.24 Air.in :compress @14:10:17.34 Air.seek :evac @14:10:20.39 2.6psi after compress @14:10:30.98 -160.1psi after compress (stable) @14:10:31.00 Air.in :default @14:10:31.09 Air.hold @14:10:31.34 at 0.9psi Opened to waste @14:10:31.35 Handoff.select :blocked @14:10:31.54 Archive syringe pumping up to 8 strokes to reach 50psi @14:10:31.56 Archive stroke #1 of 8 @14:10:31.69 Archive.in :pressurize @14:10:31.78 Archive.seek :pressurized @14:10:44.23 1.7psi after pressurize @14:10:49.82 -160.1psi after pressurize (stable) @14:10:49.85 Archive.in :compress @14:10:49.95 Archive.seek :empty @14:11:05.82 15.9psi after compress @14:11:16.41 -160.1psi after compress (stable) @14:11:16.44 Archive.in :gulp @14:11:16.55 Archive.seek :topVent @14:11:22.89 15.9psi after gulp @14:11:28.49 -160.1psi after gulp (stable) @14:11:28.50 Archive stroke #2 of 8 @14:11:28.66 Archive.in :pressurize @14:11:28.74 Archive.seek :pressurized @14:11:41.20 16.0psi after pressurize @14:11:55.81 -160.0psi after pressurize (stable) @14:11:55.84 Archive.in :compress @14:11:55.94 Archive.seek :empty @14:12:11.70 28.9psi after compress @14:12:22.29 -160.0psi after compress (stable) @14:12:22.32 Archive.in :gulp @14:12:22.44 Archive.seek :topVent @14:12:28.78 29.0psi after gulp @14:12:34.87 -160.0psi after gulp (stable) @14:12:34.88 Archive stroke #3 of 8 @14:12:35.13 Archive.in :pressurize @14:12:35.22 Archive.seek :pressurized @14:12:47.69 28.7psi after pressurize @14:12:53.28 -160.0psi after pressurize (stable) @14:12:53.31 Archive.in :compress @14:12:53.41 Archive.seek :empty @14:13:09.15 41.3psi after compress @14:13:19.74 -159.9psi after compress (stable) @14:13:19.77 Archive.in :gulp @14:13:19.89 Archive.seek :topVent @14:13:26.23 41.5psi after gulp @14:13:32.32 -159.9psi after gulp (stable) @14:13:32.34 Archive stroke #4 of 8 @14:13:32.48 Archive.in :pressurize @14:13:32.56 Archive.seek :pressurized @14:13:45.02 41.4psi after pressurize @14:13:50.61 -159.9psi after pressurize (stable) @14:13:50.64 Archive.in :compress @14:13:50.73 Archive.seek :empty @14:14:03.45 Reached 50.0 of 50.0psi after pumping 6845uL (3.96 strokes) @14:14:03.58 Archive.in :default @14:14:03.69 Archive.hold @14:14:03.80 Handoff.select :waste @14:14:03.97 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:14:14.07 delay 1:00 @14:15:14.12 Handoff.select :blocked @14:15:14.29 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:15:36.88 Handoff.select :waste @14:15:37.04 -160.1psi at evacEnd @14:15:37.05 Handoff.select :blocked @14:15:37.23 Archive.in :fast @14:15:37.30 Archive.seek :topVent @14:15:44.34 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:15:50.93 Stable @-160.1psi after Handoff blocked @14:15:50.96 Archive syringe pumping up to 8 strokes to reach 30psi @14:15:51.00 Archive stroke #1 of 8 @14:15:51.13 Archive.in :pressurize @14:15:51.21 Archive.seek :pressurized @14:16:03.68 2.3psi after pressurize @14:16:03.71 Archive.in :compress @14:16:03.82 Archive.seek :empty @14:16:19.55 13.6psi after compress @14:16:30.17 -160.1psi after compress (stable) @14:16:30.20 Archive.in :gulp @14:16:30.30 Archive.seek :topVent @14:16:36.64 13.4psi after gulp @14:16:42.23 -160.1psi after gulp (stable) @14:16:42.25 Archive stroke #2 of 8 @14:16:42.38 Archive.in :pressurize @14:16:42.48 Archive.seek :pressurized @14:16:54.93 13.4psi after pressurize @14:16:54.96 Archive.in :compress @14:16:55.06 Archive.seek :empty @14:17:10.79 25.5psi after compress @14:17:21.38 -160.0psi after compress (stable) @14:17:21.41 Archive.in :gulp @14:17:21.58 Archive.seek :topVent @14:17:27.95 25.4psi after gulp @14:17:33.54 -160.0psi after gulp (stable) @14:17:33.56 Archive stroke #3 of 8 @14:17:33.69 Archive.in :pressurize @14:17:33.77 Archive.seek :pressurized @14:17:46.22 25.1psi after pressurize @14:17:46.25 Archive.in :compress @14:17:46.35 Archive.seek :empty @14:17:52.94 Reached 29.8 of 30.0psi after pumping 4994uL (2.89 strokes) @14:17:52.97 Archive.in :default @14:17:53.07 Archive.hold @14:17:53.18 Handoff.select :waste @14:17:53.35 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:18:06.93 delay 1:00 @14:19:06.96 Handoff.select :blocked @14:19:07.26 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:19:29.85 Handoff.select :waste @14:19:30.00 -160.1psi at evacEnd @14:19:30.02 Air.seek :fullVent @14:19:31.61 Archive.in :fast @14:19:31.69 Archive.seek :topVent @14:19:38.20 Seawater evacuated, Pressure above/below filter released. @14:19:38.32 Lysis1.in :slow @14:19:38.51 Lysis1.seek 1050uL @14:20:08.15 delay 5 seconds @14:20:13.18 Handoff.select :blocked @14:20:13.41 Lysis1.seek 450uL @14:21:12.57 delay 3 seconds @14:21:15.66 Air.in mix with 1.26318 parts Lysis1 @14:21:16.36 Air.seek :evac @14:21:16.40 Lysis1.seek 300uL @14:21:31.42 Lysis1.seek :empty @14:22:01.13 Lysis buffer delivered @5.3psi @14:22:01.15 Polling Can every 2:00 @14:22:01.70 PuckHeater.configure :default #ambient 21.4C @14:22:01.79 PuckHeater.seek 79.8C @14:22:50.12 PuckHeater.hold 2:00,79.8C @14:24:49.60 Polling Can every 2:00 @14:24:50.15 PuckHeater.configure :default #ambient 21.3C @14:24:50.24 PuckHeater.hold 30 seconds,78.7C @14:25:20.33 Polling Can every 2:00 @14:25:20.88 PuckHeater.configure :default #ambient 21.3C @14:25:20.96 PuckHeater.hold 30 seconds,78.2C @14:25:51.06 Polling Can every 2:00 @14:25:51.61 PuckHeater.hold 10:00,77.7C @14:35:48.49 Lysis complete. Syringe pressure @6.8psi @14:35:48.51 Archive syringe pumping up to 5 strokes to reach 40psi @14:35:48.55 Archive stroke #1 of 5 @14:35:48.67 Archive.in :pressurize @14:35:48.74 Archive.seek :pressurized @14:36:01.64 7.5psi after pressurize @14:36:07.73 -160.1psi after pressurize (stable) @14:36:07.76 Archive.in :compress @14:36:07.86 Archive.seek :empty @14:36:23.60 20.2psi after compress @14:37:08.69 -160.1psi after compress (stable) @14:37:08.72 Archive.in :gulp @14:37:08.82 Archive.seek :topVent @14:37:15.16 1.6psi after gulp @14:37:20.75 -160.1psi after gulp (stable) @14:37:20.76 Archive stroke #2 of 5 @14:37:20.92 Archive.in :pressurize @14:37:21.01 Archive.seek :pressurized @14:37:33.46 4.0psi after pressurize @14:37:54.55 -160.1psi after pressurize (stable) @14:37:54.58 Archive.in :compress @14:37:54.68 Archive.seek :empty @14:38:10.41 2.2psi after compress @14:38:21.00 -160.1psi after compress (stable) @14:38:21.03 Archive.in :gulp @14:38:21.13 Archive.seek :topVent @14:38:27.46 1.2psi after gulp @14:38:33.56 -160.1psi after gulp (stable) @14:38:33.57 Archive stroke #3 of 5 @14:38:33.71 Archive.in :pressurize @14:38:33.80 Archive.seek :pressurized @14:38:46.24 3.8psi after pressurize @14:39:01.34 -160.1psi after pressurize (stable) @14:39:01.37 Archive.in :compress @14:39:01.47 Archive.seek :empty @14:39:17.24 2.2psi after compress @14:39:27.84 -160.1psi after compress (stable) @14:39:28.35 Archive.in :gulp @14:39:28.48 Archive.seek :topVent @14:39:34.82 1.0psi after gulp @14:39:40.41 -160.1psi after gulp (stable) @14:39:40.42 Archive stroke #4 of 5 @14:39:40.56 Archive.in :pressurize @14:39:40.65 Archive.seek :pressurized @14:39:53.10 3.7psi after pressurize @14:40:07.71 -160.1psi after pressurize (stable) @14:40:07.73 Archive.in :compress @14:40:07.84 Archive.seek :empty @14:40:23.56 2.0psi after compress @14:40:34.17 -160.1psi after compress (stable) @14:40:34.20 Archive.in :gulp @14:40:34.31 Archive.seek :topVent @14:40:40.64 0.8psi after gulp @14:40:46.23 -160.1psi after gulp (stable) @14:40:46.24 Archive stroke #5 of 5 @14:40:46.38 Archive.in :pressurize @14:40:46.46 Archive.seek :pressurized @14:40:58.91 2.6psi after pressurize @14:41:12.31 Archive.in :default @14:41:12.44 Archive.hold @14:41:12.72 UserAbort in n -- User Abort!! @14:43:08.42 -> Cartridge.unlcok @14:43:08.47 NoMethodError in n -- undefined method `unlcok' for Cartridge @14:43:26.09 -> Handoff.waste @14:43:26.15 Handoff.select :waste Handoff Valve::Manifold selects waste @14:43:30.46 -> Cartridge.unlock @14:43:30.53 Gate.power :heat,:OFF @14:43:30.55 Gate.power :cartridge,:OFF @14:43:30.68 PinPort.in :unlocking @14:43:30.75 PinPort.seek :clear @14:43:31.26 Carousel.in :default @14:43:31.41 Carousel.home.seek 43 @14:43:31.79 Archive.in :unlocking @14:43:31.93 Archive.seek :clear @14:43:32.07 Air.in :unlocking @14:43:32.74 Air.seek :clear @14:43:33.03 Lysis2.in :unlocking @14:43:33.12 Lysis2.seek :clear @14:43:37.70 Lysis1.in :unlocking @14:43:37.86 Lysis1.seek :clear @14:43:37.96 Plunge.in :unlocking @14:43:38.06 Plunge.seek :clear @14:47:14.21 -> to 41 @14:47:14.55 Carousel.seek 41 @14:47:15.29 Lysis1.in :fast @14:47:15.37 Lysis1.seek :locked @14:47:18.02 Plunge.in :default @14:47:18.12 Plunge.seek :locked @14:47:18.30 Air.in :fast @14:47:18.39 Air.seek :locked @14:47:19.39 Carousel.reconfigure :locked @14:47:19.58 Powering cartridge bus @14:47:22.24 Gate.setIR 1750,5000,0,6 @14:47:22.37 Gate.setIR 0,5000,0,6 @14:47:22.47 Gate.power :cartridge,:OFF @14:47:23.05 Mounted archive Cartridge :archive_bac @14:47:59.63 -> BACsampler.primeVolume=BACsampler.bypassVolume=10 10 @14:48:07.96 -> BACsampler.goalVolume=100 100 @14:48:14.88 -> Cartrige.sampler.source=ni @14:48:14.92 NameError in n -- uninitialized constant Cartrige @14:48:17.43 -> Cartrige.sampler.source=nil @14:48:17.46 NameError in n -- uninitialized constant Cartrige @14:48:24.45 -> Cartridge.sampler.source=nil @14:49:02.69 -> BACsampler.sample 100 @14:49:02.71 Sampling 100.0ml @14:49:02.76 SamplePump.setPosition! 0ml @14:49:02.86 Bypass.close @14:49:02.95 Exhaust.open @14:49:03.65 Intake.open @14:49:04.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:49:07.02 Vacuum tare is 53% of full scale at 21C (drifted 1%) @14:49:07.18 Abs.in :default @14:49:07.28 Abs.hold @14:49:07.62 SamplePump.in :start @14:49:07.73 SamplePump.seek 100ml,8:50 @14:49:08.44 SamplePump.configure :sample @14:51:42.66 Abs.stop @14:51:42.82 Intake.close @14:51:42.87 Exhaust.close @14:51:43.00 Handoff.select :close @14:51:43.65 Sampled 100.0ml 99.9989785495404 @14:53:47.57 -> archiveAUV @14:53:47.73 Plunge.seek :reagent @14:53:48.58 Sample loop closed. Ready to Deliver Reagents @14:53:48.61 Archive.in :default @14:53:48.72 Archive.seek :sealed @14:53:52.70 Archive.seek :evac @14:54:06.98 delay 1:00 @14:54:57.94 UserAbort in n -- User Abort!! @14:55:06.28 -> Cartridge.unlock @14:55:06.40 Carousel.in :default @14:55:06.55 Carousel.home.seek 41 @14:55:06.96 Archive.in :unlocking @14:55:07.14 Archive.seek :clear @14:55:07.20 Air.in :unlocking @14:55:07.44 Air.seek :clear @14:55:11.83 Lysis1.in :unlocking @14:55:12.01 Lysis1.seek :clear @14:55:12.10 Plunge.in :unlocking @14:55:12.24 Plunge.seek :clear @14:58:32.70 -> to 40 @14:58:33.01 Carousel.seek 40 @14:58:33.57 Lysis1.in :fast @14:58:33.66 Lysis1.seek :locked @14:58:36.31 Plunge.in :default @14:58:36.44 Plunge.seek :locked @14:58:36.58 Air.in :fast @14:58:36.67 Air.seek :locked @14:58:37.65 Carousel.reconfigure :locked @14:58:37.85 Powering cartridge bus @14:58:40.51 Gate.setIR 1750,5000,0,6 @14:58:40.64 Gate.setIR 0,5000,0,6 @14:58:40.74 Gate.power :cartridge,:OFF @14:58:41.32 Mounted archive Cartridge :archive_bac @14:58:47.12 -> BACsampler.sample 100 @14:58:47.17 Sampling 100.0ml @14:58:47.19 SamplePump.setPosition! 0ml @14:58:47.30 Exhaust.open @14:58:47.99 Intake.open @14:58:48.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:58:51.39 Vacuum tare is 53% of full scale at 21C (drifted 1%) @14:58:51.57 Abs.in :default @14:58:51.65 Abs.hold @14:58:52.61 SamplePump.in :start @14:58:52.72 SamplePump.seek 100ml,8:50 @14:58:53.30 SamplePump.configure :sample @15:01:27.97 Abs.stop @15:01:28.15 Intake.close @15:01:28.23 Exhaust.close @15:01:29.00 Sampled 100.0ml 99.9989785495404 @15:05:00.88 -> Sampler.psi -0.102414488006815 @15:05:12.60 -> Syringe.stabilize @15:05:12.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi 160.375112194645 @15:06:22.40 -> archiveRNAl @15:06:22.45 Archive.in :default @15:06:22.54 Archive.seek :sealed @15:06:26.50 Archive.seek :evac @15:06:28.70 Slide::Error in n -- Archive Syringe (ArS) positionErr at 1357uL (sensed 1388uL) @15:06:28.73 Retry #1 of 2 @15:06:28.77 Archive.hold @15:06:29.15 Archive.in :default @15:06:29.25 Archive.home.seek :evac @15:06:29.90 Slide::Error in n -- Archive Syringe (ArS) positionErr at 1357uL (sensed 1388uL) @15:06:29.92 Retry #2 of 2 @15:06:29.96 Archive.in :default @15:06:30.12 Archive.hold @15:06:30.34 Archive.in :default @15:06:30.43 Archive.home.seek :evac @15:06:31.09 Slide::Error in n -- IGNORED Archive Syringe (ArS) positionErr at 1357uL (sensed 1388uL) @15:06:31.12 Archive.in :default @15:06:31.23 Archive.hold @15:06:31.52 Archive.in :default @15:06:31.60 Archive.home.jog 0 @15:06:31.75 ArchiveError in n -- 1356ul RNAlater undelivered @15:06:36.53 -> archiveAUV @15:06:36.66 Plunge.seek :reagent @15:06:37.50 Sample loop closed. Ready to Deliver Reagents @15:06:37.53 Archive.seek :sealed @15:06:39.65 Archive.seek :evac @15:06:53.92 delay 1:00 @15:07:53.95 Archive.seek :clear @15:08:11.66 Sample preserved. Puck Full. @15:54:47.25 -> archiveEvac 2 @15:54:47.30 Archive.seek :tVent @15:54:49.85 delay 1 second @15:54:50.88 Archive.seek :evac @15:55:06.54 delay 10 seconds @15:55:16.58 Archive.seek :tVent @15:55:32.24 delay 1 second @15:55:33.27 Archive.seek :evac @15:55:48.94 delay 10 seconds 2 @16:18:27.17 -> Cartridge.unlock @16:18:27.30 Carousel.in :default @16:18:27.47 Carousel.home.seek 40 @16:18:27.85 Archive.in :unlocking @16:18:28.68 Archive.seek :clear @16:18:28.75 Air.in :unlocking @16:18:29.00 Air.seek :clear @16:18:33.37 Lysis1.in :unlocking @16:18:33.55 Lysis1.seek :clear @16:18:33.65 Plunge.in :unlocking @16:18:33.77 Plunge.seek :clear @16:19:16.32 -> slots {[34,28,15,6] => { Type: :lyseHold_phyto, State: :dry}, [53,31,30] => { Type: :archive_bac, State: :missing}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 54 => { Type: :lyseHold_phyto, State: :leaky}} @16:19:31.69 Session Begun @16:19:41.81 -> slots [53,31,30]=>nil @16:19:41.92 -> Syringe {[53,31,30] => nil} Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear @16:19:47.83 -> Syringe.psi -0.286132875877172 @16:20:59.63 -> SC.to 54 @16:20:59.66 Carousel.seek 54 Storage Carousel (SC) at 54 @16:21:30.98 -> Syringe.stabilize @16:21:31.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi 160.512266796305 @16:21:43.38 -> Syringe.psi -0.333427566104803 @16:22:58.58 -> Syringe::PressureSkes @16:22:58.61 NameError in brent -- uninitialized constant Syringe::PressureSkes @16:23:00.57 -> Syringe::PressureSkew 0.004729469(PSI/count)-160.9 @16:23:07.96 -> Syringe::PressureSkew.bias -160.894171419893 @16:23:25.25 -> Syringe::PressureSkew.bias-=50 -210.894171419893 @16:23:29.91 -> Syringe.psi -50.2861328758772 @16:23:37.64 -> Syringe.stabilize @16:23:37.68 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi 160.635232990897 @16:23:51.10 -> Syringe.psi -50.309780220991 @16:23:59.72 -> Syringe.tare @16:23:59.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:24:06.42 Syringe tare is 52% of full scale at 21C (drifted 1%) 0.004729469(PSI/count)-160.6 @16:24:14.24 -> Syringe.psi -0.0508417919946851 @16:47:37.36 Session Ended @16:52:10.11 -> to 48 @16:52:10.45 Carousel.seek 48 @16:52:12.62 Lysis1.in :fast @16:52:12.69 Lysis1.seek :locked @16:52:15.37 Plunge.in :default @16:52:15.44 Plunge.seek :locked @16:52:15.64 Air.in :fast @16:52:15.72 Air.seek :locked @16:52:16.72 Carousel.reconfigure :locked @16:52:16.90 Powering cartridge bus @16:52:19.57 Gate.setIR 1750,5000,0,6 @16:52:19.70 Gate.setIR 0,5000,0,6 @16:52:19.80 PuckHeater.configure :default #ambient 21.3C @16:52:19.89 Gate.power :heat,:ON,limit to 5000mA @16:52:20.59 Mounted lysis Cartridge board#Q05075 @16:52:20.65 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @16:52:48.28 -> PhytoSampler.prepareLGcart 0,0 @16:52:48.31 Handoff.select :waste @16:52:48.50 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:52:55.12 Syringe tare is 52% of full scale at 21C (drifted 1%) @16:52:55.15 PinPort.in :locking @16:52:55.26 PinPort.seek :locked @16:52:55.76 Archive.in :fast @16:52:55.82 Lysis2.in :fast @16:52:55.93 Lysis1.seek :empty @16:52:56.02 Archive.seek :full @16:52:56.20 Lysis2.seek :empty Archive Syringe (ArS) full @16:53:14.52 -> delay 5; Handoff.blocked @16:53:14.55 delay 5 seconds @16:53:19.60 Handoff.select :blocked Handoff Valve::Manifold selects blocked @16:53:42.74 -> Air.evac @16:53:42.79 Air.in :default @16:53:42.87 Air.seek :evac Air Syringe (AirS) at evac @16:54:06.09 -> Syringe.psi 13.2555193035496 @16:54:15.52 -> ArS.pumpTo 40 @16:54:15.56 Archive syringe pumping up to 8 strokes to reach 40psi @16:54:15.59 Archive stroke #1 of 8 @16:54:15.73 Archive.in :pressurize @16:54:15.82 Archive.seek :pressurized @16:54:28.27 13.8psi after pressurize @16:54:44.86 -159.7psi after pressurize (stable) @16:54:44.89 Archive.in :compress @16:54:45.10 Archive.seek :empty @16:55:00.82 29.2psi after compress @16:55:11.41 -159.7psi after compress (stable) @16:55:11.44 Archive.in :gulp @16:55:11.54 Archive.seek :topVent @16:55:17.86 29.3psi after gulp @16:55:23.45 -159.7psi after gulp (stable) @16:55:23.46 Archive stroke #2 of 8 @16:55:23.60 Archive.in :pressurize @16:55:23.69 Archive.seek :pressurized @16:55:36.17 28.9psi after pressurize @16:55:56.27 -159.7psi after pressurize (stable) @16:55:56.35 Archive.in :compress @16:55:56.45 Archive.seek :empty @16:56:09.23 Reached 40.1 of 40.0psi after pumping 3396uL (1.96 strokes) @16:56:09.25 Archive.in :default @16:56:09.34 Archive.hold 3396.43472409152 @16:57:10.64 -> Syringe.psi 40.3222705208234 @16:57:59.85 -> Handoff.waste @16:57:59.87 Handoff.select :waste Handoff Valve::Manifold selects waste @16:59:38.00 -> Handoff.blocked @16:59:38.02 Handoff.select :blocked Handoff Valve::Manifold selects blocked @16:59:41.28 -> ArS.pumpTo 40 @16:59:41.32 Archive syringe pumping up to 8 strokes to reach 40psi @16:59:41.36 Archive stroke #1 of 8 @16:59:41.49 Archive.in :compress @16:59:41.59 Archive.seek :empty @16:59:44.75 1.9psi after compress @16:59:55.50 -159.8psi after compress (stable) @16:59:55.57 Archive.in :gulp @16:59:55.68 Archive.seek :topVent @17:00:02.01 1.7psi after gulp @17:00:07.60 -159.8psi after gulp (stable) @17:00:07.62 Archive stroke #2 of 8 @17:00:07.75 Archive.in :pressurize @17:00:07.82 Archive.seek :pressurized @17:00:20.26 3.3psi after pressurize @17:00:32.85 -159.8psi after pressurize (stable) @17:00:32.87 Archive.in :compress @17:00:32.98 Archive.seek :empty @17:00:48.71 15.6psi after compress @17:00:59.49 -159.8psi after compress (stable) @17:00:59.54 Archive.in :gulp @17:00:59.65 Archive.seek :topVent @17:01:05.98 15.1psi after gulp @17:01:11.59 -159.8psi after gulp (stable) @17:01:11.60 Archive stroke #3 of 8 @17:01:11.73 Archive.in :pressurize @17:01:11.81 Archive.seek :pressurized @17:01:24.24 15.3psi after pressurize @17:01:38.83 -159.7psi after pressurize (stable) @17:01:38.86 Archive.in :compress @17:01:38.96 Archive.seek :empty @17:01:54.69 28.6psi after compress @17:02:05.29 -159.7psi after compress (stable) @17:02:05.41 Archive.in :gulp @17:02:05.51 Archive.seek :topVent @17:02:11.85 28.2psi after gulp @17:02:17.44 -159.7psi after gulp (stable) @17:02:17.45 Archive stroke #4 of 8 @17:02:17.58 Archive.in :pressurize @17:02:17.65 Archive.seek :pressurized @17:02:30.11 28.0psi after pressurize @17:02:45.21 -159.7psi after pressurize (stable) @17:02:45.24 Archive.in :compress @17:02:45.34 Archive.seek :empty @17:03:00.21 Reached 40.0 of 40.0psi after pumping 6901uL (3.99 strokes) @17:03:00.23 Archive.in :default @17:03:00.32 Archive.hold 6900.67429340511 @17:03:40.00 -> 5.times {puts Syringe.psi; Delay.sleep 60] @17:03:40.03 SyntaxError in n -- compile error n:36: syntax error, unexpected ']', expecting '}' 5.times {puts Syringe.psi; Delay.sleep 60] ^ @17:03:46.75 -> 5.times {puts Syringe.psi; Delay.sleep 60} 5 @17:09:42.24 -> Handoff.waste @17:09:42.27 Handoff.select :waste Handoff Valve::Manifold selects waste @01:34:45.70PDT17-Jul-24 Session Begun @01:35:02.89 -> reload PressSensor [/home/esp/esp2/lib/sensor.rb] @01:35:18.91 -> Syringe.stabilize @01:35:18.95 Waiting up to 3:00 for Syringe to stabilize +/-42.29psi 33943.25 @01:37:56.18 -> reload PressSensor [/home/esp/esp2/lib/sensor.rb] @01:38:00.12 -> Syringe.stabilize @01:38:00.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi 33931.75 @01:38:19.22 -> Syringe.psi -0.0342886504150499 @01:38:24.62 -> Syringe.tare @01:38:24.68 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:38:31.32 Syringe tare is 52% of full scale at 20C (drifted 1%) 0.004729469(PSI/count)-160.4 @01:38:40.77 -> Syringe.psi 0.016553141579692 @01:38:47.28 -> Syringe.tare @01:38:47.34 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:38:53.98 Syringe tare is 52% of full scale at 20C (drifted 1%) 0.004729469(PSI/count)-160.5 @01:38:58.87 -> Syringe.psi 0.0922246459438725 @01:40:55.62 -> Handoff.blocked @01:40:55.65 Handoff.select :blocked Handoff Valve::Manifold selects blocked @01:41:14.76 -> Archive.pumpTo 40 @01:41:14.82 Archive syringe pumping up to 8 strokes to reach 40psi @01:41:14.84 Archive stroke #1 of 8 @01:41:14.99 Archive.in :compress @01:41:15.09 Archive.seek :empty @01:41:16.20 1.5psi after compress @01:41:26.80 0.5psi after compress (stable) @01:41:26.83 Archive.in :gulp @01:41:26.96 Archive.seek :topVent @01:41:33.30 1.1psi after gulp @01:41:38.89 0.5psi after gulp (stable) @01:41:39.01 Archive stroke #2 of 8 @01:41:39.18 Archive.in :pressurize @01:41:39.28 Archive.seek :pressurized @01:41:51.73 1.9psi after pressurize @01:42:06.84 7.2psi after pressurize (stable) @01:42:06.87 Archive.in :compress @01:42:07.03 Archive.seek :empty @01:42:22.77 13.7psi after compress @01:42:33.36 12.9psi after compress (stable) @01:42:33.39 Archive.in :gulp @01:42:33.53 Archive.seek :topVent @01:42:39.97 13.8psi after gulp @01:42:40.05 Errno::EPIPE in brent's_reader -- Broken pipe @01:42:40.15 Archive.in :default @01:42:40.22 Archive.hold @01:42:40.34 Session Ended @01:46:21.45 Session Begun @01:46:28.19 -> showlog 30 @01:46:46.15 -> Archive.pumpTo 40 @01:46:46.19 Archive syringe pumping up to 8 strokes to reach 40psi @01:46:46.24 Archive stroke #1 of 8 @01:46:46.41 Archive.in :pressurize @01:46:46.52 Archive.seek :pressurized @01:46:58.98 13.4psi after pressurize @01:47:04.58 12.8psi after pressurize (stable) @01:47:04.65 Archive.in :compress @01:47:04.74 Archive.seek :empty @01:47:20.49 26.3psi after compress @01:47:31.08 25.6psi after compress (stable) @01:47:31.11 Archive.in :gulp @01:47:31.24 Archive.seek :topVent @01:47:37.57 26.6psi after gulp @01:47:43.17 25.8psi after gulp (stable) @01:47:43.18 Archive stroke #2 of 8 @01:47:43.34 Archive.in :pressurize @01:47:43.44 Archive.seek :pressurized @01:47:55.89 26.2psi after pressurize @01:48:01.48 25.5psi after pressurize (stable) @01:48:01.60 Archive.in :compress @01:48:01.78 Archive.seek :empty @01:48:17.52 38.6psi after compress @01:48:28.14 37.8psi after compress (stable) @01:48:28.17 Archive.in :gulp @01:48:28.30 Archive.seek :topVent @01:48:34.64 38.8psi after gulp @01:48:40.24 38.0psi after gulp (stable) @01:48:40.26 Archive stroke #3 of 8 @01:48:40.43 Archive.in :pressurize @01:48:40.53 Archive.seek :pressurized @01:48:52.98 38.6psi after pressurize @01:48:58.57 37.8psi after pressurize (stable) @01:48:58.60 Archive.in :compress @01:48:58.74 Archive.seek :empty @01:49:04.26 Reached 40.1 of 40.0psi after pumping 4971uL (2.87 strokes) @01:49:04.29 Archive.in :default @01:49:04.43 Archive.hold 4970.89771197847 @01:49:32.66 -> Syringe.psi 42.2980062050827 @01:49:39.85 -> Syringe.counts 42879 @01:55:31.86 -> Handoff.waste @01:55:31.89 Handoff.select :waste Handoff Valve::Manifold selects waste @01:55:36.71 -> Syringe.psi -0.0354710176707158 @01:55:41.34 -> exit @01:55:41.37 Session Ended @07:46:57.05 -> MainThread.exit @07:46:57.21 Handoff.select :close # @07:46:57.47 Session Ended @08:12:01.99PDT17-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @08:12:04.40 >BREAK< ESP3 Gateway v1.43 for core bus @08:12:04.42 >BREAK< ESP3 Gateway v1.43 for cartridge bus @08:12:08.14 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @08:12:08.82 Sea pressure (Abs) tare is 6% of full scale @08:12:12.67 Polling Can every 2:00 @08:12:12.74 Waiting for espclients... @08:12:13.58 Session Begun @08:12:24.23 -> to 54 @08:12:24.55 Carousel.configure :default @08:12:24.68 Carousel.seek 54 @08:12:26.26 Lysis1.configure :fast @08:12:26.35 Lysis1.seek :locked @08:12:28.99 Plunge.configure :default @08:12:29.16 Plunge.seek :locked @08:12:29.28 Air.configure :fast @08:12:29.38 Air.seek :locked @08:12:30.37 Carousel.reconfigure :locked @08:12:30.61 Gate.power :sampler,:OFF,limit to 3500mA @08:12:30.64 Gate.power :skimmer,:OFF,limit to 3500mA @08:12:30.68 Gate.power :analytic1,:ON,limit to 4000mA @08:12:31.39 Plunge.hold @08:12:31.51 Plunge.configure :default @08:12:31.67 SamplePump.configure :default @08:12:31.86 Toroid.configure :default @08:12:31.96 Toroid.hold @08:12:32.10 Toroid.configure :default @08:12:32.32 Carousel.hold @08:12:32.44 Carousel.configure :locked @08:12:32.62 Abs.configure :default @08:12:33.06 PhaseSeparator.configure :default @08:12:33.26 Archive.configure :default @08:12:33.35 Archive.hold @08:12:33.46 Archive.configure :default @08:12:33.64 Air.in :default @08:12:33.72 Air.hold @08:12:33.85 Air.configure :default @08:12:34.00 Lysis1.in :default @08:12:34.12 Lysis1.hold @08:12:34.24 Lysis1.configure :default @08:12:34.42 Lysis2.configure :default @08:12:34.78 Lysis2.hold @08:12:34.89 Lysis2.configure :default @08:12:35.07 PinPort.configure :default @08:12:35.17 PinPort.hold @08:12:35.27 PinPort.configure :default @08:12:35.37 All elf controllers running firmware version 4.92 @08:12:36.20 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:12:36.23 AnalyticModule::Unknown in preston -- SPR @08:12:36.41 Powering cartridge bus @08:12:39.16 Gate.setIR 1750,5000,0,6 @08:12:39.29 Gate.setIR 0,5000,0,6 @08:12:39.47 PuckHeater.configure :default @08:12:39.56 Gate.power :heat,:ON,limit to 5000mA @08:12:40.34 Mounted lysis Cartridge board#Q05092 @08:12:40.41 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @08:12:49.26 -> Cartridge.sampler.source=nil @08:12:58.08 -> fire lyseHold_phyto # @08:12:59.09 Toroid.seek :clear @08:12:59.30 Carousel.in :default @08:12:59.44 Carousel.home.seek 54 @08:12:59.90 Carousel.reconfigure :locked @08:13:00.20 Gate.setIR 1750,5000,0,6 @08:13:00.33 Gate.setIR 0,5000,0,6 @08:13:00.42 PuckHeater.configure :default @08:13:00.52 Gate.power :heat,:ON,limit to 5000mA @08:13:03.20 Mounted lysis Cartridge board#Q05092 @08:13:03.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:13:03.34 Selecting Cartridge 54 @08:13:03.77 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 54" @08:13:04.15 Preparing to filter cartridge 54 @08:13:04.55 Air.seek :sealed @08:13:04.75 Priming sample loop w/150ml, bypass w/50ml @08:13:04.79 SamplePump.setPosition! 0ml @08:13:04.94 Exhaust.close @08:13:05.17 Bypass.open @08:13:06.73 Intake.open @08:13:07.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:13:08.21 upsync @08:13:10.32 Vacuum tare is 52% of full scale at 21C (drifted 1%) @08:13:10.50 Abs.in :default @08:13:11.06 Abs.hold @08:13:11.41 SamplePump.in :start @08:13:11.51 SamplePump.seek 50ml,3:50 @08:13:12.11 SamplePump.configure :prime @08:13:35.05 -> PhytoSampler.primeVolume=PhytoSampler.bypassVolume=10 10 @08:14:12.91 Abs.stop @08:14:13.01 SamplePump.setPosition! 0ml @08:14:13.08 Exhaust.open @08:14:14.45 Bypass.close @08:14:16.02 Abs.in :default @08:14:16.16 Abs.hold @08:14:16.50 SamplePump.in :start @08:14:16.61 SamplePump.seek 150ml,10:30 @08:14:17.20 SamplePump.configure :prime @08:17:20.49 Abs.stop @08:17:20.59 Bypass.open @08:17:21.31 Intake.close @08:17:22.06 Toroid.in :hold @08:17:22.20 Toroid.seek :crack @08:17:23.78 Toroid.seek :cartridge @08:17:24.90 Toroid.in :default @08:17:25.00 Toroid.seek :clear @08:17:27.07 Pressurizing cartridge to 26psi above 15.7psia @08:17:27.09 SamplePump.setPosition! 0ml @08:17:27.20 Intake.open @08:17:27.87 Bypass.close @08:17:28.57 Exhaust.close @08:17:29.38 Abs.in :default @08:17:29.47 Abs.hold @08:17:29.73 SamplePump.in :charge @08:17:29.82 SamplePump.seek 2.3ml,42 seconds @08:17:46.91 Abs.stop @08:17:47.01 Intake.close @08:17:47.75 Pressurized cartridge with 1.315ml to 27.6psi @08:17:47.77 Isolated @28.2psia (12.4psi) @08:17:47.81 Verifying cartridge holds at least 0.65..0.85 of 28.2psia @08:17:47.87 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:17:52.59 Toroid.in :hold @08:17:52.69 Toroid.seek :bypass @08:17:54.37 Toroid.in :default @08:17:54.47 Toroid.seek :clear @08:17:56.74 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 36% to 18.0psia @08:17:56.77 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @08:17:56.82 BadNews.email "Isolated cartridge pressure fell by 36% to 18.0psia",Subject: "Cartridge::Sampler::Leaky in FILTERING" @08:17:59.37 upsync @08:19:50.45 Session Begun @08:20:00.14 -> fire lyseHold_phyto @08:20:00.35 Selecting Cartridge 34 # @08:20:00.66 Gate.power :heat,:OFF @08:20:00.69 Gate.power :cartridge,:OFF @08:20:00.88 Carousel.in :default @08:20:01.09 Carousel.home.seek 54 @08:20:01.56 Air.in :unlocking @08:20:01.76 Air.seek :clear @08:20:03.28 Lysis1.in :unlocking @08:20:03.46 Lysis1.seek :clear @08:20:03.58 Plunge.in :unlocking @08:20:03.72 Plunge.seek :clear @08:20:04.91 Carousel.seek 34 @08:20:09.36 Lysis1.in :fast @08:20:09.47 Lysis1.seek :locked @08:20:12.19 Plunge.in :default @08:20:12.33 Plunge.seek :locked @08:20:12.50 Air.in :fast @08:20:12.61 Air.seek :locked @08:20:13.63 Carousel.reconfigure :locked @08:20:13.85 Powering cartridge bus @08:20:16.51 Gate.setIR 1750,5000,0,6 @08:20:16.64 Gate.setIR 0,5000,0,6 @08:20:16.76 PuckHeater.configure :default @08:20:16.88 Gate.power :heat,:ON,limit to 5000mA @08:20:17.58 Mounted lysis Cartridge board#Q05087 @08:20:17.66 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:20:17.72 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 34" @08:20:18.31 Preparing to filter cartridge 34 @08:20:19.58 Air.in :default @08:20:19.69 Bypass.open @08:20:19.95 Air.seek :sealed @08:20:20.23 Cmd::Error in detached from preston -- Cannot process while in PRIMING state @08:20:20.26 Waiting to Resume from Checkpoint "Selecting Cartridge 54" @08:20:20.51 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 54" @08:20:21.14 Toroid.in :hold @08:20:21.48 Toroid.seek :crack @08:20:21.69 upsync @08:20:22.81 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from preston" @08:20:23.21 Toroid.seek :cartridge @08:20:23.83 upsync @08:20:24.53 Toroid.in :default @08:20:24.67 Toroid.seek :clear @08:20:25.78 upsync @08:20:26.68 Exhaust.open @08:20:27.62 Pressurizing cartridge to 26psi above 15.6psia @08:20:27.64 SamplePump.setPosition! 0ml @08:20:27.76 Intake.open @08:20:28.46 Bypass.close @08:20:29.17 Exhaust.close @08:20:29.96 Abs.in :default @08:20:30.07 Abs.hold @08:20:30.40 SamplePump.seek 2.3ml,42 seconds @08:20:44.19 Abs.stop @08:20:44.31 Intake.close @08:20:45.07 Pressurized cartridge with 0.851ml to 26.9psi @08:20:45.09 Isolated @38.7psia (23.0psi) @08:20:45.13 Verifying cartridge holds at least 0.65..0.85 of 38.7psia @08:20:45.27 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:20:49.95 Sampling 50.0ml @08:20:49.98 SamplePump.setPosition! 0ml @08:20:50.15 Exhaust.open @08:20:50.83 Intake.open @08:20:51.72 Abs.in :default @08:20:51.81 Abs.hold @08:20:52.23 SamplePump.in :start @08:20:52.35 SamplePump.seek 50ml,4:40 @08:20:52.92 SamplePump.configure :sample @08:20:55.34 Cartridge 34 filtering 2/50ml at 0.42ml/s with 19.5psi vacuum @08:21:55.39 Cartridge 34 filtering 38/50ml at 0.61ml/s with 17.0psi vacuum @08:22:15.37 Abs.stop @08:22:15.59 Intake.close @08:22:15.72 Exhaust.close @08:22:15.99 Toroid.in :hold @08:22:16.13 Toroid.seek :bypass @08:22:17.82 Toroid.in :default @08:22:17.94 Toroid.seek :clear @08:22:19.62 Sampled 50.0ml @08:22:20.14 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 34" @08:22:20.70 Handoff.select :waste @08:22:20.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:22:22.94 upsync @08:22:27.56 Syringe tare is 52% of full scale at 22C (drifted 1%) @08:22:27.59 PinPort.in :locking @08:22:27.75 PinPort.seek :locked @08:22:28.30 Archive.in :fast @08:22:28.97 Lysis2.in :fast @08:22:29.07 Lysis1.seek 1350uL @08:22:29.12 Archive.seek :full @08:22:29.40 Lysis2.seek :empty @08:22:37.49 Plunge.seek :reagent @08:22:38.45 Evacuating puck with filter bubble point of 50psi @08:22:38.48 PinPort.seek :locked @08:22:38.67 Handoff.select :waste @08:22:38.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:22:45.35 Air syringe pumping up to 1 strokes to reach 8psi @08:22:45.37 Air stroke #1 of 1 @08:22:45.55 Air.in :pressurize @08:22:45.67 Air.seek :pressurized @08:22:51.34 1.1psi after pressurize @08:22:51.37 Air.in :compress @08:22:51.52 Air.seek :evac @08:22:54.54 3.6psi after compress @08:23:05.13 0.4psi after compress (stable) @08:23:05.15 Air.in :default @08:23:05.31 Air.hold @08:23:05.57 at 0.3psi Opened to waste @08:23:05.59 Handoff.select :blocked @08:23:05.80 Archive syringe pumping up to 8 strokes to reach 50psi @08:23:05.81 Archive stroke #1 of 8 @08:23:05.99 Archive.in :pressurize @08:23:06.11 Archive.seek :pressurized @08:23:18.60 1.6psi after pressurize @08:23:24.19 1.0psi after pressurize (stable) @08:23:24.22 Archive.in :compress @08:23:24.37 Archive.seek :empty @08:23:40.18 2.4psi after compress @08:23:50.76 1.0psi after compress (stable) @08:23:50.79 Archive.in :gulp @08:23:50.94 Archive.seek :topVent @08:23:57.29 2.0psi after gulp @08:24:02.88 1.3psi after gulp (stable) @08:24:02.90 Archive stroke #2 of 8 @08:24:03.09 Archive.in :pressurize @08:24:03.20 Archive.seek :pressurized @08:24:15.66 2.1psi after pressurize @08:24:21.25 1.4psi after pressurize (stable) @08:24:21.27 Archive.in :compress @08:24:21.42 Archive.seek :empty @08:24:37.21 2.8psi after compress @08:24:47.80 1.4psi after compress (stable) @08:24:47.82 Archive.in :gulp @08:24:47.98 Archive.seek :topVent @08:24:48.41 -> Archive.stop @08:24:48.43 Archive.stop @08:24:48.78 Archive.in :default @08:24:48.92 Archive.hold Archive Syringe (ArS) at 105uL @08:24:49.15 Handoff.select :blocked @08:24:49.20 Slide::Error in PROCESSING -- Archive Syringe (ArS) aborted at 105uL (sensed 107uL) @08:24:49.26 Waiting to Resume from Checkpoint "Archive Syringe (ArS) aborted at 105uL (sensed 107uL)" @08:24:49.32 BadNews.email "Archive Syringe (ArS) aborted at 105uL (sensed 107uL)",Subject: "Slide::Error in PROCESSING" @08:24:51.71 upsync @08:24:58.74 -> ArS.empty @08:24:58.81 Archive.seek :empty Archive Syringe (ArS) empty @08:25:06.95 -> ArS.jog -100 @08:25:07.00 Archive.jog -100 Archive Syringe (ArS) at -23uL @08:25:15.23 -> ArS.full @08:25:15.29 Archive.seek :full @08:25:27.95 Archive.hold @08:25:28.64 UserAbort in preston -- Archive Syringe (ArS) seek to full Aborted! @08:25:36.52 -> ArS.empty @08:25:36.58 Archive.seek :empty Archive Syringe (ArS) empty @08:25:56.92 -> ArS.jog -100 @08:25:57.01 Archive.jog -100 Archive Syringe (ArS) at -23uL @08:26:31.43 -> ArS.full @08:26:31.49 Archive.seek :full Archive Syringe (ArS) full @08:27:14.97 -> ArS.empty; ArS.jog -100; ArS.full :fast @08:27:15.00 Archive.seek :empty @08:27:30.74 Archive.jog -100 @08:27:31.45 Archive.in :fast @08:27:31.55 Archive.seek :full Archive Syringe (ArS) full @08:27:58.46 -> Thread[:PROCESSING].resume @08:27:58.52 Resuming Thread after Archive Syringe (ArS) aborted at 105uL (sensed 107uL) at 08:24:48 # @08:27:58.62 Archive.in :gulp @08:27:58.81 Archive.seek :topVent @08:27:59.01 1.9psi after gulp @08:28:04.61 1.7psi after gulp (stable) @08:28:04.63 Archive stroke #3 of 8 @08:28:04.84 Archive.in :pressurize @08:28:04.95 Archive.seek :pressurized @08:28:06.19 Archive.in :default @08:28:06.30 Archive.hold @08:28:06.44 Handoff.select :blocked @08:28:06.58 Archive.setRawPosition! -1457 @08:28:06.70 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 1611uL (sensed 1635uL) @08:28:06.73 Waiting to Resume from Checkpoint "Archive Syringe (ArS) positionErr at 1611uL (sensed 1635uL)" @08:28:06.82 BadNews.email "Archive Syringe (ArS) positionErr at 1611uL (sensed 1635uL)",Subject: "Slide::Error in PROCESSING" @08:28:08.96 upsync @08:28:21.40 -> ArS.home.jog 0 @08:28:21.44 Archive.in :default @08:28:21.57 Archive.home.jog 0 Archive Syringe (ArS) at 1624uL @08:28:24.37 -> Thread[:PROCESSING].resume # @08:28:24.47 Resuming Thread after Archive Syringe (ArS) positionErr at 1611uL (sensed 1635uL) at 08:28:05 @08:28:24.55 Archive.in :pressurize @08:28:24.72 Archive.seek :pressurized @08:28:36.28 2.5psi after pressurize @08:28:55.89 8.8psi after pressurize (stable) @08:28:55.92 Archive.in :compress @08:28:56.08 Archive.seek :empty @08:29:11.90 16.3psi after compress @08:29:22.49 16.0psi after compress (stable) @08:29:22.52 Archive.in :gulp @08:29:22.73 Archive.seek :topVent @08:29:29.09 17.0psi after gulp @08:29:34.68 16.4psi after gulp (stable) @08:29:34.69 Archive stroke #4 of 8 @08:29:34.87 Archive.in :pressurize @08:29:34.95 Archive.seek :pressurized @08:29:47.42 17.0psi after pressurize @08:29:53.03 16.4psi after pressurize (stable) @08:29:53.09 Archive.in :compress @08:29:53.21 Archive.seek :empty @08:30:04.83 -> ArS.stop @08:30:04.86 Archive.stop @08:30:05.11 Archive.in :default @08:30:05.23 Archive.hold @08:30:05.40 Handoff.select :blocked Archive Syringe (ArS) at 84uL @08:30:05.50 Slide::Error in PROCESSING -- Archive Syringe (ArS) aborted at 84uL (sensed 86uL) @08:30:05.56 Waiting to Resume from Checkpoint "Archive Syringe (ArS) aborted at 84uL (sensed 86uL)" @08:30:05.66 BadNews.email "Archive Syringe (ArS) aborted at 84uL (sensed 86uL)",Subject: "Slide::Error in PROCESSING" @08:30:08.63 upsync @08:30:17.35 -> ArS.empty, ArS.jog -100 @08:30:17.41 SyntaxError in preston -- compile error preston:17: syntax error, unexpected tUMINUS_NUM, expecting tCOLON2 or '[' or '.' ArS.empty, ArS.jog -100 ^ @08:30:25.84 -> ArS.empty; ArS.jog -100 @08:30:25.91 Archive.seek :empty @08:30:27.20 Archive.jog -100 Archive Syringe (ArS) at -23uL @08:30:38.76 -> ArS.full :fast @08:30:38.82 Archive.in :fast @08:30:38.97 Archive.seek :full Archive Syringe (ArS) full @08:30:58.08 -> Thread[:PROCESSING].resume @08:30:58.20 Resuming Thread after Archive Syringe (ArS) aborted at 84uL (sensed 86uL) at 08:30:04 # @08:30:58.30 Archive.in :compress @08:30:58.47 Archive.seek :empty @08:32:19.54 29.0psi after compress @08:32:30.16 28.8psi after compress (stable) @08:32:30.19 Archive.in :gulp @08:32:30.36 Archive.seek :topVent @08:32:36.71 29.5psi after gulp @08:32:42.30 28.7psi after gulp (stable) @08:32:42.32 Archive stroke #5 of 8 @08:32:42.51 Archive.in :pressurize @08:32:42.62 Archive.seek :pressurized @08:32:55.10 28.3psi after pressurize @08:33:01.69 28.5psi after pressurize (stable) @08:33:01.72 Archive.in :compress @08:33:01.88 Archive.seek :empty @08:33:17.63 40.9psi after compress @08:33:28.22 40.9psi after compress (stable) @08:33:28.25 Archive.in :gulp @08:33:28.39 Archive.seek :topVent @08:33:34.74 41.5psi after gulp @08:33:36.13 -> slots {[28,15,6] => { Type: :lyseHold_phyto, State: :dry}, 34 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50.0}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}, 54 => { Type: :lyseHold_phyto, State: :leaky}} @08:33:40.32 40.5psi after gulp (stable) @08:33:40.34 Archive stroke #6 of 8 @08:33:40.53 Archive.in :pressurize @08:33:40.61 Archive.seek :pressurized @08:33:53.11 39.8psi after pressurize @08:34:00.14 39.8psi after pressurize (stable) @08:34:00.22 Archive.in :compress @08:34:00.35 Archive.seek :empty @08:34:14.32 Reached 48.8 of 50.0psi after pumping 10339uL (5.98 strokes) @08:34:14.36 Archive.in :default @08:34:14.51 Archive.hold @08:34:14.65 Handoff.select :waste @08:34:14.83 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:34:26.92 delay 1:00 @08:35:26.95 Handoff.select :blocked @08:35:27.15 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @08:35:49.74 Handoff.select :waste @08:35:49.93 1.2psi at evacEnd @08:35:49.95 Handoff.select :blocked @08:35:50.15 Archive.in :fast @08:35:50.25 Archive.seek :topVent @08:35:57.42 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @08:36:04.01 Stable @1.1psi after Handoff blocked @08:36:04.04 Archive syringe pumping up to 8 strokes to reach 31psi @08:36:04.12 Archive stroke #1 of 8 @08:36:04.31 Archive.in :pressurize @08:36:04.41 Archive.seek :pressurized @08:36:16.88 2.0psi after pressurize @08:36:16.91 Archive.in :compress @08:36:17.07 Archive.seek :empty @08:36:32.82 15.0psi after compress @08:36:43.42 14.4psi after compress (stable) @08:36:43.45 Archive.in :gulp @08:36:43.60 Archive.seek :topVent @08:36:49.96 15.2psi after gulp @08:36:55.63 14.5psi after gulp (stable) @08:36:55.64 Archive stroke #2 of 8 @08:36:55.83 Archive.in :pressurize @08:36:55.93 Archive.seek :pressurized @08:37:08.39 14.8psi after pressurize @08:37:08.42 Archive.in :compress @08:37:08.57 Archive.seek :empty @08:37:24.33 27.1psi after compress @08:37:34.93 26.5psi after compress (stable) @08:37:34.96 Archive.in :gulp @08:37:35.12 Archive.seek :topVent @08:37:41.47 27.3psi after gulp @08:37:47.06 26.7psi after gulp (stable) @08:37:47.07 Archive stroke #3 of 8 @08:37:47.25 Archive.in :pressurize @08:37:47.36 Archive.seek :pressurized @08:37:59.82 26.4psi after pressurize @08:37:59.86 Archive.in :compress @08:37:59.99 Archive.seek :empty @08:38:07.75 Reached 31.1 of 31.1psi after pumping 5018uL (2.90 strokes) @08:38:07.79 Archive.in :default @08:38:07.92 Archive.hold @08:38:08.60 Handoff.select :waste @08:38:08.89 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:38:18.99 delay 1:00 @08:39:19.01 Handoff.select :blocked @08:39:19.22 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @08:39:42.33 Handoff.select :waste @08:39:42.52 0.9psi at evacEnd @08:39:42.55 Air.seek :fullVent @08:39:44.19 Archive.in :fast @08:39:44.32 Archive.seek :topVent @08:39:50.93 Seawater evacuated, Pressure above/below filter released. @08:39:51.08 Lysis1.in :slow @08:39:51.28 Lysis1.seek 1050uL @08:40:20.95 delay 5 seconds @08:40:26.00 Handoff.select :blocked @08:40:26.30 Lysis1.seek 450uL @08:41:25.47 delay 3 seconds @08:41:28.57 Air.in mix with 1.26318 parts Lysis1 @08:41:29.31 Air.seek :evac @08:41:29.39 Lysis1.seek 300uL @08:41:44.40 Lysis1.seek :empty @08:42:14.15 Lysis buffer delivered @8.8psi @08:42:14.17 Polling Can every 2:00 @08:42:14.76 PuckHeater.configure :default #ambient 21.6C @08:42:14.87 PuckHeater.seek 79.8C @08:43:08.57 PuckHeater.hold 2:00,79.8C @08:45:08.80 Polling Can every 2:00 @08:45:09.35 PuckHeater.configure :default #ambient 21.6C @08:45:09.46 PuckHeater.hold 30 seconds,78.7C @08:45:39.73 Polling Can every 2:00 @08:45:40.28 PuckHeater.configure :default #ambient 21.6C @08:45:40.39 PuckHeater.hold 30 seconds,78.2C @08:46:10.66 Polling Can every 2:00 @08:46:11.21 PuckHeater.configure :default #ambient 21.6C @08:46:11.38 PuckHeater.hold 10:00,77.7C @08:56:11.87 Lysis complete. Syringe pressure @3.8psi @08:56:12.00 Archive syringe pumping up to 5 strokes to reach 40psi @08:56:12.07 Archive stroke #1 of 5 @08:56:12.28 Archive.in :pressurize @08:56:12.39 Archive.seek :pressurized @08:56:24.86 6.2psi after pressurize @08:56:42.45 17.7psi after pressurize (stable) @08:56:42.48 Archive.in :compress @08:56:42.63 Archive.seek :empty @08:56:58.40 36.1psi after compress @08:57:08.99 35.3psi after compress (stable) @08:57:09.02 Archive.in :gulp @08:57:09.18 Archive.seek :topVent @08:57:15.54 35.8psi after gulp @08:57:21.14 35.3psi after gulp (stable) @08:57:21.15 Archive stroke #2 of 5 @08:57:21.34 Archive.in :pressurize @08:57:21.44 Archive.seek :pressurized @08:57:33.90 35.1psi after pressurize @08:57:39.49 34.4psi after pressurize (stable) @08:57:39.58 Archive.in :compress @08:57:39.74 Archive.seek :empty @08:57:44.71 Reached 40.1 of 40.0psi after pumping 3229uL (1.87 strokes) @08:57:44.73 Archive.in :default @08:57:44.87 Archive.hold @08:57:45.01 delay 2 seconds @08:57:47.04 Archive.in :fast @08:57:47.14 Archive.seek :full @08:57:53.61 Lysis1.seek 16uL @08:57:55.31 delay 1 second @08:57:56.38 Archive.seek :full @08:57:56.43 Lysis1.seek :empty @08:57:58.23 Handoff.select :blocked @08:57:58.47 Initial air pressure @42.0psi @08:57:58.61 Lysis1.in :extract @08:57:58.80 Lysis1.seek 600uL,nil @08:58:04.48 Initial 600uL lysate @17.3psi @08:58:04.51 Handoff.select :blocked @08:58:04.68 Initial air pressure @17.2psi @08:58:04.78 Lysis1.seek 825uL,nil @08:58:06.24 Archive syringe pumping up to 6 strokes to reach 17psi @08:58:06.26 Archive stroke #1 of 6 @08:58:06.45 Archive.in :pressurize @08:58:06.59 Archive.seek :pressurized @08:58:08.17 Archive.in :default @08:58:08.30 Archive.hold @08:58:08.87 Recovered 825uL lysate @15.5psi @08:58:08.90 Air.in :halfSpeed @08:58:09.05 Air.seek :fullVent @08:58:11.53 Puck Top Vented, bottom @5.8psi @08:58:11.55 Handoff.select :waste @08:58:11.75 delay 5 seconds @08:58:16.83 Handoff open to waste, bottom @0.4psi @08:58:16.85 Air.in :default @08:58:17.00 Air.seek :evac @08:58:18.67 Archive.stop @08:58:18.87 Handoff.select :close @08:58:19.07 Gate.power :heat,:OFF @08:58:19.10 Gate.power :cartridge,:OFF @08:58:19.28 PinPort.in :unlocking @08:58:19.38 PinPort.seek :clear @08:58:19.90 Carousel.in :default @08:58:20.07 Carousel.home.seek 34 @08:58:20.49 Plunge.seek :locked @08:58:21.44 Archive.in :unlocking @08:58:21.58 Archive.seek :clear @08:58:21.76 Air.in :unlocking @08:58:21.92 Air.seek :clear @08:58:22.12 Lysis1.in :unlocking @08:58:22.28 Lysis1.seek :clear @08:58:22.45 Lysis2.in :unlocking @08:58:22.58 Lysis2.seek :clear @08:58:27.23 Gate.power :analytic1,:OFF @08:58:27.26 Gate.power :motor,:OFF @08:58:27.32 Gate.power :core,:OFF @08:58:27.35 Gate.power :main,:OFF @08:58:27.40 Safely stopped and ready to power off @09:10:45.26 -> exit @09:10:45.87 Session Ended @09:15:11.06 Session Begun @09:15:16.95 -> MainThread.exit # @09:15:17.14 ThreadError in tp -- killed thread @09:15:17.28 Session Ended @09:15:17.35 Session Ended @09:15:28.14PDT17-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @09:15:30.61 >BREAK< ESP3 Gateway v1.43 for core bus @09:15:30.64 >BREAK< ESP3 Gateway v1.43 for cartridge bus @09:15:34.53 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @09:15:35.27 Sea pressure (Abs) tare is 6% of full scale @09:15:39.32 Polling Can every 2:00 @09:15:39.37 Waiting for espclients... @09:15:39.92 Session Begun @09:15:40.29 -> PhytoSampler.xray @09:16:07.10 -> slots {[28,15,6] => { Type: :lyseHold_phyto, State: :dry}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}, 54 => { Type: :lyseHold_phyto, State: :leaky}} @09:16:40.76 -> exit @09:16:40.78 Session Ended @09:31:55.34 Session Begun Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @09:32:46.90 -> slots {[28,15,6] => { Type: :lyseHold_phyto, State: :dry}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}, 54 => { Type: :lyseHold_phyto, State: :leaky}} @09:33:18.85 -> fire lyseHold_phyto @09:33:19.05 Gate.power :sampler,:OFF,limit to 3500mA # @09:33:19.09 Gate.power :skimmer,:OFF,limit to 3500mA @09:33:19.16 Gate.power :main,:ON,limit to 5000mA @09:33:19.21 Gate.power :analytic1,:ON,limit to 4000mA @09:33:19.41 Powering core bus @09:33:22.22 Plunge.configure :default @09:33:22.33 SamplePump.configure :default @09:33:22.44 Toroid.configure :default @09:33:22.59 Carousel.configure :default @09:33:22.71 Abs.configure :default @09:33:22.82 PhaseSeparator.configure :default @09:33:22.93 Archive.configure :default @09:33:23.04 Air.configure :default @09:33:23.38 Lysis1.configure :default @09:33:23.46 Lysis2.configure :default @09:33:23.57 PinPort.configure :default @09:33:23.65 All elf controllers running firmware version 4.92 @09:33:23.69 Gate.power :motor,:ON,limit to 5000mA @09:33:26.58 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @09:33:26.61 AnalyticModule::Unknown in detached from n -- SPR @09:33:26.81 Plunge.home.jog 0 @09:33:27.61 PinPort.home.jog 0 @09:33:27.66 Lysis1.home.jog 0 @09:33:27.71 Carousel.home.jog 0 @09:33:27.77 Air.home.jog 0 @09:33:27.81 Archive.home.jog 0 @09:33:27.86 Toroid.home.jog 0 @09:33:27.90 Lysis2.home.jog 0 @09:33:28.84 Plunge.in :homing @09:33:28.98 Plunge.jog 1612 @09:33:29.20 Lysis2.in :homing @09:33:29.26 Lysis1.in :homing @09:33:29.30 Archive.in :homing @09:33:29.36 PinPort.in :homing @09:33:29.41 Air.in :homing @09:33:29.46 Toroid.in :homing @09:33:29.78 Lysis2.jog 1404 @09:33:29.82 Archive.jog 1376 @09:33:29.90 Lysis1.jog 1394 @09:33:29.97 PinPort.jog -105 @09:33:30.01 Air.jog 1372 @09:33:30.17 Toroid.jog 633 @09:33:30.54 Plunge.setRawPosition! 50 @09:33:30.62 Plunge.seek :home @09:33:30.74 PinPort.setRawPosition! 0 @09:33:30.82 PinPort.seek :home @09:33:30.98 PinPort.in :default @09:33:31.00 Plunge.in :default @09:33:31.29 Lysis2.setRawPosition! 50 @09:33:31.38 Lysis2.seek :home @09:33:31.91 Lysis1.setRawPosition! 50 @09:33:31.97 Archive.setRawPosition! 50 @09:33:32.16 Lysis1.seek :home @09:33:32.18 Archive.seek :home @09:33:32.51 Lysis2.in :default @09:33:32.58 Air.setRawPosition! 50 @09:33:32.72 Air.seek :home @09:33:32.87 Lysis1.in :default @09:33:32.90 Archive.in :default @09:33:32.94 Toroid.setRawPosition! 0 @09:33:33.16 Toroid.seek :home @09:33:33.31 Toroid.in :default @09:33:33.34 Air.in :default @09:33:33.79 Toroid.seek :clear @09:33:35.48 Carousel.in :default @09:33:35.63 Carousel.home.seek 34 @09:33:35.93 Selecting Cartridge 28 @09:33:36.28 Carousel.seek 28 @09:33:37.88 Lysis1.in :fast @09:33:37.97 Lysis1.seek :locked @09:33:40.62 Plunge.seek :locked @09:33:40.81 Air.in :fast @09:33:40.90 Air.seek :locked @09:33:41.91 Carousel.reconfigure :locked @09:33:42.10 Powering cartridge bus @09:33:44.78 Gate.setIR 1750,5000,0,6 @09:33:44.91 Gate.setIR 0,5000,0,6 @09:33:45.01 PuckHeater.configure :default @09:33:45.10 Gate.power :heat,:ON,limit to 5000mA @09:33:45.87 Mounted lysis Cartridge board#Q05061 @09:33:45.94 Using this cartridge's heat loss curve for HeatingAccelInCarousel @09:33:46.03 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 28" @09:33:46.46 Preparing to filter cartridge 28 @09:33:46.84 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @09:33:49.92 upsync @09:33:53.61 Scale::Skew::CannotTare in FILTERING -- Intake Head Pressure sensor @31.3% scale out of usable range @09:33:53.63 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi" @09:33:53.68 BadNews.email "Intake Head Pressure sensor @31.3% scale out of usable range",Subject: "Scale::Skew::CannotTare in FILTERING" @09:33:56.01 upsync @09:34:46.15 -> Cartridge.lock @09:34:46.22 Gate.power :heat,:OFF @09:34:46.24 Gate.power :cartridge,:OFF @09:34:47.82 Powering cartridge bus @09:34:50.51 Gate.setIR 1750,5000,0,6 @09:34:50.65 Gate.setIR 0,5000,0,6 @09:34:50.74 PuckHeater.configure :default @09:34:50.83 Gate.power :heat,:ON,limit to 5000mA @09:34:51.56 Mounted lysis Cartridge board#Q05061 @09:34:51.62 Using this cartridge's heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @09:34:53.82 -> Cartridge.sampler.source=nil @09:34:58.77 -> fire lyseHold_phyto @09:34:58.94 Selecting Cartridge 28 # @09:34:59.42 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 28" @09:34:59.96 Preparing to filter cartridge 28 @09:35:00.66 Air.in :default @09:35:00.96 Air.seek :sealed @09:35:01.14 Priming sample loop w/150ml, bypass w/50ml @09:35:01.19 Cmd::Error in detached from n -- Cannot process while in PRIMING state @09:35:01.26 SamplePump.setPosition! 0ml @09:35:01.28 Waiting to Resume from Checkpoint "Selecting Cartridge 28" @09:35:01.41 Bypass.open @09:35:01.63 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 28" @09:35:02.45 upsync @09:35:03.27 Intake.open @09:35:04.00 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n" @09:35:04.56 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:35:05.14 upsync @09:35:06.95 upsync @09:35:07.25 Vacuum tare is 53% of full scale at 21C (drifted 1%) @09:35:07.46 Abs.in :default @09:35:07.55 Abs.hold @09:35:07.83 SamplePump.in :start @09:35:07.92 SamplePump.seek 50ml,3:50 @09:35:08.52 SamplePump.configure :prime @09:36:09.31 Abs.stop @09:36:09.41 SamplePump.setPosition! 0ml @09:36:09.51 Exhaust.open @09:36:10.92 Bypass.close @09:36:12.44 Abs.in :default @09:36:12.53 Abs.hold @09:36:12.88 SamplePump.in :start @09:36:12.99 SamplePump.seek 150ml,10:30 @09:36:13.57 SamplePump.configure :prime @09:39:16.88 Abs.stop @09:39:16.98 Bypass.open @09:39:17.70 Intake.close @09:39:18.50 Toroid.in :hold @09:39:18.59 Toroid.seek :crack @09:39:20.17 Toroid.seek :cartridge @09:39:21.32 Toroid.in :default @09:39:21.41 Toroid.seek :clear @09:39:23.49 Pressurizing cartridge to 26psi above 15.7psia @09:39:23.51 SamplePump.setPosition! 0ml @09:39:23.62 Intake.open @09:39:24.31 Bypass.close @09:39:25.00 Exhaust.close @09:39:25.76 Abs.in :default @09:39:25.85 Abs.hold @09:39:26.13 SamplePump.in :charge @09:39:26.30 SamplePump.seek 2.3ml,42 seconds @09:39:38.42 SamplePump.stop @09:39:38.52 Intake.close @09:39:38.78 Toroid.in :hold @09:39:38.88 Toroid.seek :bypass @09:39:40.56 Toroid.in :default @09:39:40.66 Toroid.seek :clear @09:39:42.31 Abs.stop @09:39:42.60 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only 0.5psi vacuum after 0.7ml @09:39:42.63 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @09:39:42.67 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only 0.5psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @09:39:44.85 upsync @09:40:52.59 -> SC Storage Carousel (SC) at 28 @09:40:58.82 -> slot 28=>:dry {28 => { Type: :lyseHold_phyto, State: :dry}} @09:41:04.17 -> fire lyseHold_phyto @09:41:04.37 Selecting Cartridge 28 # @09:41:04.85 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 28" @09:41:05.28 Preparing to filter cartridge 28 @09:41:05.93 Air.seek :sealed @09:41:06.10 Bypass.open @09:41:06.44 Cmd::Error in detached from n#2 -- Cannot process while in PRIMING state @09:41:06.47 Waiting to Resume from Checkpoint "Selecting Cartridge 28" @09:41:07.06 Toroid.in :hold @09:41:07.31 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 28" @09:41:07.37 Toroid.seek :crack @09:41:08.66 upsync @09:41:09.46 Toroid.seek :cartridge @09:41:09.89 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n#2" @09:41:10.71 Toroid.in :default @09:41:10.87 upsync @09:41:10.93 Toroid.seek :clear @09:41:12.47 upsync @09:41:12.82 Exhaust.open @09:41:13.76 Pressurizing cartridge to 26psi above 15.7psia @09:41:13.79 SamplePump.setPosition! 0ml @09:41:13.89 Intake.open @09:41:14.55 Bypass.close @09:41:15.22 Exhaust.close @09:41:16.04 Abs.in :default @09:41:16.16 Abs.hold @09:41:16.43 SamplePump.seek 2.3ml,42 seconds @09:41:28.54 SamplePump.stop @09:41:28.65 Intake.close @09:41:28.92 Toroid.in :hold @09:41:29.02 Toroid.seek :bypass @09:41:30.70 Toroid.in :default @09:41:30.81 Toroid.seek :clear @09:41:32.46 Abs.stop @09:41:32.77 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only 0.5psi vacuum after 0.7ml @09:41:32.80 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @09:41:32.86 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only 0.5psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @09:41:35.10 upsync @09:42:22.12 -> fire lyseHold_phyto @09:42:22.35 Selecting Cartridge 15 # @09:42:22.61 Gate.power :heat,:OFF @09:42:22.63 Gate.power :cartridge,:OFF @09:42:23.29 Carousel.in :default @09:42:23.44 Carousel.home.seek 28 @09:42:23.90 Air.in :unlocking @09:42:24.04 Air.seek :clear @09:42:25.55 Lysis1.in :unlocking @09:42:25.72 Lysis1.seek :clear @09:42:25.85 Plunge.in :unlocking @09:42:25.96 Plunge.seek :clear @09:42:27.11 Carousel.seek 15 @09:42:30.16 Lysis1.in :fast @09:42:30.25 Lysis1.seek :locked @09:42:32.91 Plunge.in :default @09:42:33.05 Plunge.seek :locked @09:42:33.20 Air.in :fast @09:42:33.30 Air.seek :locked @09:42:34.31 Carousel.reconfigure :locked @09:42:34.50 Powering cartridge bus @09:42:37.17 Gate.setIR 1750,5000,0,6 @09:42:37.31 Gate.setIR 0,5000,0,6 @09:42:37.41 PuckHeater.configure :default @09:42:37.51 Gate.power :heat,:ON,limit to 5000mA @09:42:38.27 Mounted lysis Cartridge board#Q05067 @09:42:38.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:42:38.39 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 15" @09:42:38.90 Preparing to filter cartridge 15 @09:42:39.47 Air.in :default @09:42:39.66 Bypass.open @09:42:39.74 Air.seek :sealed @09:42:40.05 Cmd::Error in detached from n#3 -- Cannot process while in PRIMING state @09:42:40.07 Waiting to Resume from Checkpoint "Selecting Cartridge 28" @09:42:40.60 Toroid.in :hold @09:42:40.82 Toroid.seek :crack @09:42:40.88 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 28" @09:42:42.32 upsync @09:42:43.21 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from n#3" @09:42:43.53 Toroid.seek :cartridge @09:42:44.22 upsync @09:42:44.81 Toroid.in :default @09:42:44.90 Toroid.seek :clear @09:42:45.76 upsync @09:42:46.77 Exhaust.open @09:42:47.68 Pressurizing cartridge to 26psi above 15.7psia @09:42:47.71 SamplePump.setPosition! 0ml @09:42:47.82 Intake.open @09:42:48.51 Bypass.close @09:42:49.19 Exhaust.close @09:42:49.97 Abs.in :default @09:42:50.05 Abs.hold @09:42:50.35 SamplePump.seek 2.3ml,42 seconds @09:43:04.97 Abs.stop @09:43:05.07 Intake.close @09:43:05.83 Pressurized cartridge with 0.960ml to 26.0psi @09:43:05.85 Isolated @36.8psia (21.1psi) @09:43:05.89 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @09:43:05.95 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:43:10.58 Cartridge pressure fell 7% to 34.4psia @09:43:10.71 Sampling 50.0ml @09:43:10.73 SamplePump.setPosition! 0ml @09:43:10.83 Exhaust.open @09:43:11.49 Intake.open @09:43:12.36 Abs.in :default @09:43:12.45 Abs.hold @09:43:12.80 SamplePump.in :start @09:43:12.91 SamplePump.seek 50ml,4:40 @09:43:13.49 SamplePump.configure :sample @09:43:16.48 Cartridge 15 filtering 2/50ml at 0.65ml/s with 19.3psi vacuum 4.0m deep (20.6psia) @09:44:16.53 Cartridge 15 filtering 39/50ml at 0.62ml/s with 25.9psi vacuum @09:44:33.67 Abs.stop @09:44:33.86 Intake.close @09:44:33.91 Exhaust.close @09:44:34.24 Toroid.in :hold @09:44:34.33 Toroid.seek :bypass @09:44:36.00 Toroid.in :default @09:44:36.13 Toroid.seek :clear @09:44:37.79 Sampled 50.0ml @09:44:38.28 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 15" @09:44:38.90 Handoff.select :waste @09:44:39.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:44:40.71 upsync @09:44:45.76 Syringe tare is 52% of full scale at 21C (drifted 1%) @09:44:45.79 PinPort.in :locking @09:44:45.91 PinPort.seek :locked @09:44:46.44 Archive.in :fast @09:44:46.50 Lysis2.in :fast @09:44:46.61 Lysis1.seek 1350uL @09:44:46.76 Archive.seek :full @09:44:46.92 Lysis2.seek :empty @09:44:54.99 Plunge.seek :reagent @09:44:55.92 Evacuating puck with filter bubble point of 24psi @09:44:55.96 PinPort.seek :locked @09:44:56.14 Handoff.select :waste @09:44:56.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:45:02.86 Air syringe pumping up to 1 strokes to reach 4psi @09:45:02.88 Air stroke #1 of 1 @09:45:03.14 Air.in :pressurize @09:45:03.22 Air.seek :pressurized @09:45:08.85 0.9psi after pressurize @09:45:08.88 Air.in :compress @09:45:09.01 Air.seek :evac @09:45:12.06 2.0psi after compress @09:45:22.65 0.0psi after compress (stable) @09:45:22.67 Air.in :default @09:45:22.78 Air.hold @09:45:23.03 at 0.1psi Opened to waste @09:45:23.05 Handoff.select :blocked @09:45:23.21 Archive syringe pumping up to 8 strokes to reach 24psi @09:45:23.23 Archive stroke #1 of 8 @09:45:23.37 Archive.in :pressurize @09:45:23.46 Archive.seek :pressurized @09:45:35.92 9.6psi after pressurize @09:45:41.52 9.0psi after pressurize (stable) @09:45:41.55 Archive.in :compress @09:45:41.67 Archive.seek :empty @09:45:57.41 21.4psi after compress @09:46:01.47 Reached 24.1 of 24.0psi after pumping 1730uL (1.00 strokes) @09:46:01.50 Archive.in :default @09:46:01.60 Archive.hold @09:46:01.70 Handoff.select :waste @09:46:02.41 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:46:15.51 delay 1:00 @09:47:15.54 Handoff.select :blocked @09:47:15.76 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @09:47:38.36 Handoff.select :waste @09:47:38.52 0.3psi at evacEnd @09:47:38.54 Handoff.select :blocked @09:47:38.72 Archive.in :fast @09:47:38.81 Archive.seek :topVent @09:47:46.12 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @09:47:52.71 Stable @0.6psi after Handoff blocked @09:47:52.74 Archive syringe pumping up to 8 strokes to reach 25psi @09:47:52.79 Archive stroke #1 of 8 @09:47:52.93 Archive.in :pressurize @09:47:53.02 Archive.seek :pressurized @09:48:05.49 0.9psi after pressurize @09:48:05.52 Archive.in :compress @09:48:05.62 Archive.seek :empty @09:48:21.38 14.7psi after compress @09:48:31.97 14.2psi after compress (stable) @09:48:32.00 Archive.in :gulp @09:48:32.15 Archive.seek :topVent @09:48:38.48 15.5psi after gulp @09:48:44.07 15.0psi after gulp (stable) @09:48:44.09 Archive stroke #2 of 8 @09:48:44.27 Archive.in :pressurize @09:48:44.37 Archive.seek :pressurized @09:48:56.82 15.1psi after pressurize @09:48:56.85 Archive.in :compress @09:48:56.96 Archive.seek :empty @09:49:07.27 Reached 24.4 of 24.6psi after pumping 3341uL (1.93 strokes) @09:49:07.29 Archive.in :default @09:49:07.40 Archive.hold @09:49:07.50 Handoff.select :waste @09:49:07.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:49:15.25 delay 1:00 @09:50:15.28 Handoff.select :blocked @09:50:15.45 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @09:50:38.04 Handoff.select :waste @09:50:38.24 0.6psi at evacEnd @09:50:38.26 Air.seek :fullVent @09:50:39.86 Archive.in :fast @09:50:39.95 Archive.seek :topVent @09:50:46.73 Seawater evacuated, Pressure above/below filter released. @09:50:46.86 Lysis1.in :slow @09:50:47.03 Lysis1.seek 1050uL @09:51:16.67 delay 5 seconds @09:51:21.70 Handoff.select :blocked @09:51:21.93 Lysis1.seek 450uL @09:52:21.08 delay 3 seconds @09:52:24.20 Air.in mix with 1.26318 parts Lysis1 @09:52:24.92 Air.seek :evac @09:52:24.99 Lysis1.seek 300uL @09:52:39.96 Lysis1.seek :empty @09:53:09.67 Lysis buffer delivered @15.1psi @09:53:09.69 Polling Can every 2:00 @09:53:10.27 PuckHeater.configure :default #ambient 21.2C @09:53:10.36 PuckHeater.seek 79.8C @09:54:00.80 PuckHeater.hold 2:00,79.8C @09:56:00.75 Polling Can every 2:00 @09:56:01.29 PuckHeater.configure :default #ambient 21.2C @09:56:01.39 PuckHeater.hold 30 seconds,78.7C @09:56:31.56 Polling Can every 2:00 @09:56:32.15 PuckHeater.hold 30 seconds,78.2C @09:57:02.34 Polling Can every 2:00 @09:57:02.89 PuckHeater.configure :default #ambient 21.3C @09:57:02.99 PuckHeater.hold 10:00,77.7C @10:07:01.92 Lysis complete. Syringe pressure @19.1psi @10:07:01.95 Archive syringe pumping up to 5 strokes to reach 40psi @10:07:01.99 Archive stroke #1 of 5 @10:07:02.15 Archive.in :pressurize @10:07:02.76 Archive.seek :pressurized @10:07:15.19 18.0psi after pressurize @10:07:20.78 17.9psi after pressurize (stable) @10:07:20.81 Archive.in :compress @10:07:20.91 Archive.seek :empty @10:07:28.58 Reached 40.3 of 40.0psi after pumping 1555uL (0.90 strokes) @10:07:28.60 Archive.in :default @10:07:28.71 Archive.hold @10:07:28.82 delay 2 seconds @10:07:30.85 Archive.in :fast @10:07:30.94 Archive.seek :full @10:07:37.58 Lysis1.seek 16uL @10:07:39.29 delay 1 second @10:07:40.33 Archive.seek :full @10:07:40.37 Lysis1.seek :empty @10:07:42.16 Handoff.select :blocked @10:07:42.37 Initial air pressure @44.8psi @10:07:42.47 Lysis1.in :extract @10:07:42.61 Lysis1.seek 600uL,nil @10:07:43.92 Archive syringe pumping up to 6 strokes to reach 25psi @10:07:43.93 Archive stroke #1 of 6 @10:07:44.18 Archive.in :pressurize @10:07:44.30 Archive.seek :pressurized @10:07:56.80 17.5psi after pressurize @10:07:57.10 Archive.in :default @10:07:57.18 Archive.hold @10:07:57.37 Initial 600uL lysate @17.1psi @10:07:57.39 Handoff.select :blocked @10:07:57.64 Initial air pressure @17.6psi @10:07:57.75 Lysis1.seek 825uL,nil @10:07:58.49 Archive syringe pumping up to 6 strokes to reach 17psi @10:07:58.51 Archive stroke #1 of 6 @10:07:58.66 Archive.in :compress @10:07:58.78 Archive.seek :empty @10:07:59.13 Reached 17.5 of 17.0psi after pumping 1398uL (0.81 strokes) @10:07:59.16 Archive.in :default @10:07:59.28 Archive.hold @10:08:00.22 Archive syringe pumping up to 6 strokes to reach 17psi @10:08:00.24 Archive stroke #1 of 6 @10:08:00.39 Archive.in :compress @10:08:00.50 Archive.seek :empty @10:08:00.85 Reached 18.2 of 17.0psi after pumping 1398uL (0.81 strokes) @10:08:00.88 Archive.in :default @10:08:00.97 Archive.hold @10:08:01.95 Archive syringe pumping up to 6 strokes to reach 17psi @10:08:01.97 Archive stroke #1 of 6 @10:08:02.14 Archive.in :compress @10:08:02.25 Archive.seek :empty @10:08:02.63 Archive.in :default @10:08:02.70 Archive.hold @10:08:03.01 Recovered 825uL lysate @17.2psi @10:08:03.04 Air.in :halfSpeed @10:08:03.14 Air.seek :fullVent @10:08:05.59 Puck Top Vented, bottom @3.7psi @10:08:05.61 Handoff.select :waste @10:08:05.79 delay 5 seconds @10:08:10.86 Handoff open to waste, bottom @-0.0psi @10:08:10.89 Air.in :default @10:08:11.00 Air.seek :evac @10:08:12.74 Handoff.select :close @10:08:12.92 Gate.power :heat,:OFF @10:08:12.95 Gate.power :cartridge,:OFF @10:08:13.08 PinPort.in :unlocking @10:08:13.16 PinPort.seek :clear @10:08:13.66 Carousel.in :default @10:08:14.40 Carousel.home.seek 15 @10:08:14.77 Plunge.seek :locked @10:08:15.70 Archive.in :unlocking @10:08:15.84 Archive.seek :clear @10:08:15.98 Air.in :unlocking @10:08:16.16 Air.seek :clear @10:08:16.31 Lysis1.in :unlocking @10:08:16.48 Lysis1.seek :clear @10:08:16.54 Lysis2.in :unlocking @10:08:16.70 Lysis2.seek :clear @10:08:21.37 Gate.power :analytic1,:OFF @10:08:21.40 Gate.power :motor,:OFF @10:08:21.46 Gate.power :core,:OFF @10:08:21.48 Gate.power :main,:OFF @10:08:21.50 Safely stopped and ready to power off @10:24:09.53 -> Cartridge.unlock @10:24:09.62 BusOff in n -- core bus was not powered while reading Handoff Pin Port (PP)'s status @10:24:13.70 -> ESP.ready! @10:24:13.77 Gate.power :sampler,:OFF,limit to 3500mA @10:24:13.80 Gate.power :skimmer,:OFF,limit to 3500mA @10:24:13.84 Gate.power :main,:ON,limit to 5000mA @10:24:13.90 Gate.power :analytic1,:ON,limit to 4000mA @10:24:14.01 Powering core bus @10:24:16.79 Plunge.configure :default @10:24:16.90 SamplePump.configure :default @10:24:17.01 Toroid.configure :default @10:24:17.12 Carousel.configure :default @10:24:17.24 Abs.configure :default @10:24:17.35 PhaseSeparator.configure :default @10:24:17.46 Archive.configure :default @10:24:17.56 Air.configure :default @10:24:17.64 Lysis1.configure :default @10:24:17.72 Lysis2.configure :default @10:24:18.09 PinPort.configure :default @10:24:18.21 All elf controllers running firmware version 4.92 @10:24:18.24 Gate.power :motor,:ON,limit to 5000mA @10:24:21.26 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:24:21.29 AnalyticModule::Unknown in n -- SPR @10:24:21.54 Plunge.home.jog 0 @10:24:22.42 PinPort.home.jog 0 @10:24:22.47 Lysis1.home.jog 0 @10:24:22.53 Archive.home.jog 0 @10:24:22.57 Carousel.home.jog 0 @10:24:22.62 Toroid.home.jog 0 @10:24:22.68 Lysis2.home.jog 0 @10:24:22.74 Air.home.jog 0 @10:24:23.60 Plunge.in :homing @10:24:23.88 Plunge.jog 1616 @10:24:24.13 Lysis1.in :homing @10:24:24.18 Air.in :homing @10:24:24.24 Toroid.in :homing @10:24:24.29 Lysis2.in :homing @10:24:24.33 Archive.in :homing @10:24:24.37 PinPort.in :homing @10:24:24.65 Lysis1.jog 1394 @10:24:25.34 Air.jog 1371 @10:24:25.38 Lysis2.jog 1406 @10:24:25.43 Archive.jog 1405 @10:24:25.48 PinPort.jog -105 @10:24:25.55 Toroid.jog 616 @10:24:26.04 PinPort.setRawPosition! 0 @10:24:26.07 Plunge.setRawPosition! 50 @10:24:26.26 PinPort.seek :home @10:24:26.28 Plunge.seek :home @10:24:26.53 PinPort.in :default @10:24:26.70 Plunge.in :default @10:24:26.82 Lysis1.setRawPosition! 50 @10:24:26.91 Lysis1.seek :home @10:24:27.06 Lysis2.setRawPosition! 50 @10:24:27.10 Air.setRawPosition! 50 @10:24:27.15 Archive.setRawPosition! 50 @10:24:27.34 Lysis2.seek :home @10:24:27.39 Air.seek :home @10:24:27.42 Archive.seek :home @10:24:27.74 Lysis1.in :default @10:24:27.88 Lysis2.in :default @10:24:27.92 Air.in :default @10:24:28.13 Archive.in :default @10:24:28.17 Toroid.setRawPosition! 0 @10:24:28.33 Toroid.seek :home @10:24:28.45 Toroid.in :default @10:24:28.60 Toroid.seek :clear @10:24:30.32 Carousel.in :default @10:24:30.48 Carousel.home.seek 15 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 15 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:24:32.46 -> SC.to 28 @10:24:32.52 Carousel.seek 28 Storage Carousel (SC) at 28 @10:40:49.17 -> slots {6 => { Type: :lyseHold_phyto, State: :dry}, 15 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @10:40:57.81 -> Cartridge.unlock @10:40:57.94 Carousel.in :default @10:40:58.12 Carousel.home.seek 28 @10:42:11.06 -> SC.to 15 @10:42:11.10 Carousel.seek 15 Storage Carousel (SC) at 15 @10:42:40.75 -> SC.to 28 @10:42:40.81 Carousel.seek 28 Storage Carousel (SC) at 28 @10:44:01.82 -> slots {6 => { Type: :lyseHold_phyto, State: :dry}, 15 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @10:44:53.98 -> fire lyseHold_phyto # @10:44:55.53 Toroid.seek :clear @10:44:55.72 Carousel.in :default @10:44:55.86 Carousel.home.seek 28 @10:44:56.20 Selecting Cartridge 6 @10:44:56.53 Carousel.seek 6 @10:45:01.35 Lysis1.in :fast @10:45:01.44 Lysis1.seek :locked @10:45:04.10 Plunge.seek :locked @10:45:04.30 Air.in :fast @10:45:04.40 Air.seek :locked @10:45:05.41 Carousel.reconfigure :locked @10:45:05.59 Powering cartridge bus @10:45:08.26 Gate.setIR 1750,5000,0,6 @10:45:08.40 Gate.setIR 0,5000,0,6 @10:45:08.50 Gate.power :cartridge,:OFF @10:45:09.07 Mounted archive Cartridge @10:45:09.19 Cartridge::Incompatible in detached from n#3 -- archive sensed when expecting lyseHold_phyto in slot 6 @10:45:09.21 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @10:45:09.25 BadNews.email "archive sensed when expecting lyseHold_phyto in slot 6",Subject: "Cartridge::Incompatible in detached from n#3" @10:45:12.38 upsync @10:46:48.84 -> slot {6 => { Type: :lyseHold_phyto, State: :incompatible, sensed: :archive, was: :dry}, 15 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @10:48:00.00 -> Cartridge.unlock @10:48:00.16 Carousel.in :default @10:48:00.31 Carousel.home.seek 6 @10:48:00.76 Air.in :unlocking @10:48:00.93 Air.seek :clear @10:48:01.96 Lysis1.in :unlocking @10:48:02.16 Lysis1.seek :clear @10:48:02.24 Plunge.in :unlocking @10:48:02.40 Plunge.seek :clear @10:49:34.82 -> slot 6=>:dry {6 => { Type: :lyseHold_phyto, State: :dry, sensed: :archive, was: :dry}} @10:49:37.45 -> slot {6 => { Type: :lyseHold_phyto, State: :dry, sensed: :archive, was: :dry}, 15 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @10:50:04.32 -> slot 6=>nil {6 => nil} @10:50:28.69 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @10:50:31.48 -> slot {6 => { Type: :lyseHold_phyto, State: :dry}, 15 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, 34 => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @10:51:00.13 -> fire lyseHold_phyto # @10:51:01.13 Toroid.seek :clear @10:51:01.34 Carousel.in :default @10:51:01.48 Carousel.home.seek 6 @10:51:01.77 Selecting Cartridge 6 @10:51:02.14 Carousel.seek 6 @10:51:02.42 Lysis1.in :fast @10:51:02.51 Lysis1.seek :locked @10:51:05.18 Plunge.in :default @10:51:05.32 Plunge.seek :locked @10:51:05.48 Air.in :fast @10:51:05.57 Air.seek :locked @10:51:06.57 Carousel.reconfigure :locked @10:51:06.76 Powering cartridge bus @10:51:09.44 Gate.setIR 1750,5000,0,6 @10:51:09.57 Gate.setIR 0,5000,0,6 @10:51:09.67 PuckHeater.configure :default #ambient 21.3C @10:51:09.77 Gate.power :heat,:ON,limit to 5000mA @10:51:10.48 Mounted lysis Cartridge board#Q05031 @10:51:10.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:51:10.64 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @10:51:11.07 Preparing to filter cartridge 6 @10:51:11.44 Air.in :default @10:51:11.69 Air.seek :sealed @10:51:11.78 Priming sample loop w/150ml, bypass w/50ml @10:51:11.90 SamplePump.setPosition! 0ml @10:51:12.12 Bypass.open @10:51:13.20 upsync @10:51:13.63 Intake.open @10:51:15.03 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:51:17.65 Vacuum tare is 52% of full scale at 21C (drifted 1%) @10:51:17.84 Abs.in :default @10:51:17.93 Abs.hold @10:51:18.24 SamplePump.in :start @10:51:18.35 SamplePump.seek 50ml,3:50 @10:51:18.93 SamplePump.configure :prime @10:52:19.71 Abs.stop @10:52:19.79 SamplePump.setPosition! 0ml @10:52:19.88 Exhaust.open @10:52:21.26 Bypass.close @10:52:22.81 Abs.in :default @10:52:22.92 Abs.hold @10:52:23.25 SamplePump.in :start @10:52:23.36 SamplePump.seek 150ml,10:30 @10:52:23.94 SamplePump.configure :prime @10:55:27.01 Abs.stop @10:55:27.12 Bypass.open @10:55:27.82 Intake.close @10:55:28.63 Toroid.in :hold @10:55:28.74 Toroid.seek :crack @10:55:30.33 Toroid.seek :cartridge @10:55:31.47 Toroid.in :default @10:55:31.56 Toroid.seek :clear @10:55:33.62 Pressurizing cartridge to 26psi above 15.7psia @10:55:33.64 SamplePump.setPosition! 0ml @10:55:33.75 Intake.open @10:55:34.41 Bypass.close @10:55:35.12 Exhaust.close @10:55:35.92 Abs.hold @10:55:36.23 SamplePump.in :charge @10:55:36.32 SamplePump.seek 2.3ml,42 seconds @10:55:48.43 SamplePump.stop @10:55:48.54 Intake.close @10:55:49.38 Toroid.in :hold @10:55:49.49 Toroid.seek :bypass @10:55:51.17 Toroid.in :default @10:55:51.30 Toroid.seek :clear @10:55:52.96 Abs.stop @10:55:53.27 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only 0.6psi vacuum after 0.7ml @10:55:53.30 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @10:55:53.36 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only 0.6psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @10:55:59.74 upsync @11:06:06.78 -> Thread[:FILTERING].resuem @11:06:06.83 NoMethodError in n -- undefined method `resuem' for # @11:06:09.95 -> Thread[:FILTERING].resume @11:06:09.99 Resuming Thread after Sample Pump (SP) pressureOutOfBounds at 0.66ml at 10:55:39 # @11:06:10.14 SamplePump.seek 2.3ml,42 seconds @11:06:11.40 Abs.stop @11:06:11.56 Pressurized cartridge with 0.661ml to 26.9psi @11:06:11.57 Isolated @15.6psia (-0.1psi) @11:06:11.60 Verifying cartridge holds at least 0.65..0.85 of 15.6psia @11:06:11.66 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:06:16.42 Toroid.in :hold @11:06:16.52 Toroid.seek :cartridge @11:06:18.38 Toroid.in :default @11:06:18.48 Toroid.seek :clear @11:06:20.33 Sampling 50.0ml @11:06:20.35 SamplePump.setPosition! 0ml @11:06:20.48 Exhaust.open @11:06:21.14 Intake.open @11:06:22.05 Abs.in :default @11:06:22.18 Abs.hold @11:06:22.53 SamplePump.in :start @11:06:22.64 SamplePump.seek 50ml,4:40 @11:06:23.22 SamplePump.configure :sample @11:06:25.63 Cartridge 6 filtering 1/50ml at 0.25ml/s with 1.5psi vacuum @11:06:27.78 SamplePump.stop @11:06:27.88 Intake.close @11:06:27.98 Exhaust.close @11:06:28.91 Toroid.in :hold @11:06:29.01 Toroid.seek :bypass @11:06:30.71 Toroid.in :default @11:06:30.82 Toroid.seek :clear @11:06:32.47 Abs.stop @11:06:32.98 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.25ml/s made only 0.9psi vacuum after 1.2ml @11:06:33.00 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 1.2ml" @11:06:33.05 BadNews.email "Serious Leak or Stall -- 0.25ml/s made only 0.9psi vacuum after 1.2ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @11:06:35.32 upsync @11:07:05.98 -> Cartridge.unlock @11:07:06.04 Gate.power :heat,:OFF @11:07:06.07 Gate.power :cartridge,:OFF @11:07:06.23 Carousel.in :default @11:07:06.40 Carousel.home.seek 6 @11:07:06.85 Air.in :unlocking @11:07:07.01 Air.seek :clear @11:07:08.47 Lysis1.in :unlocking @11:07:08.64 Lysis1.seek :clear @11:07:08.74 Plunge.in :unlocking @11:07:08.85 Plunge.seek :clear @11:12:40.55 -> Cartridge.lock @11:12:40.75 Lysis1.in :fast @11:12:40.84 Lysis1.seek :locked @11:12:43.49 Plunge.in :default @11:12:43.61 Plunge.seek :locked @11:12:43.79 Air.in :fast @11:12:43.88 Air.seek :locked @11:12:44.89 Carousel.reconfigure :locked @11:12:45.08 Powering cartridge bus @11:12:47.76 Gate.setIR 1750,5000,0,6 @11:12:47.89 Gate.setIR 0,5000,0,6 @11:12:47.99 PuckHeater.configure :default #ambient 21.3C @11:12:48.11 Gate.power :heat,:ON,limit to 5000mA @11:12:48.83 Mounted lysis Cartridge board#Q05031 @11:12:48.89 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @11:12:51.78 -> Thread[:FILTERING].resume # @11:12:51.85 Resuming Thread after Sample Pump (SP) pressureOutOfBounds at 1.2ml at 11:06:25 @11:12:51.94 SamplePump.seek 50ml,4:40 @11:12:53.78 Flow averaged 0.00ml/s over 6:32 @11:12:54.57 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @11:12:54.60 Abs.stop @11:12:54.88 TypeError in FILTERING -- can't convert nil into Float @11:12:54.91 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 1.22ml" @11:12:54.96 BadNews.email "can't convert nil into Float",Subject: "TypeError in FILTERING" @11:12:58.25 upsync @11:13:04.24 -> MainThread.exit # @11:13:04.52 Session Ended @11:13:32.43PDT17-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @11:13:34.11 >BREAK< ESP3 Gateway v1.43 for core bus @11:13:34.15 >BREAK< ESP3 Gateway v1.43 for cartridge bus @11:13:37.53 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @11:13:38.22 Sea pressure (Abs) tare is 6% of full scale @11:13:42.02 Polling Can every 2:00 @11:13:42.10 Waiting for espclients... @11:13:42.64 Session Begun @11:13:48.56 -> slots {6 => { Type: :lyseHold_phyto, State: :leaky, filtered: 1.2}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @11:14:18.01 -> slot 6=>:dry {6 => { Type: :lyseHold_phyto, State: :dry, filtered: 1.2}} @11:14:36.90 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @11:14:38.76 -> to 6 @11:14:38.89 Gate.power :heat,:OFF @11:14:39.00 Carousel.configure :default @11:14:39.30 Gate.power :sampler,:OFF,limit to 3500mA @11:14:39.33 Gate.power :skimmer,:OFF,limit to 3500mA @11:14:39.38 Gate.power :analytic1,:ON,limit to 4000mA @11:14:39.89 Gate.power :cartridge,:OFF @11:14:40.26 Plunge.configure :default @11:14:40.36 Plunge.hold @11:14:40.47 Plunge.configure :default @11:14:40.67 SamplePump.configure :default @11:14:40.87 Toroid.configure :default @11:14:40.99 Toroid.hold @11:14:41.12 Toroid.configure :default @11:14:41.33 Carousel.hold @11:14:41.70 Carousel.configure :default @11:14:41.89 Abs.configure :default @11:14:42.14 PhaseSeparator.configure :default @11:14:42.35 Archive.configure :default @11:14:42.55 Air.configure :default @11:14:42.64 Air.hold @11:14:42.80 Air.configure :default @11:14:42.99 Lysis1.configure :default @11:14:43.10 Lysis1.hold @11:14:43.48 Lysis1.configure :default @11:14:43.64 Lysis2.configure :default @11:14:43.83 PinPort.configure :default @11:14:43.93 All elf controllers running firmware version 4.92 @11:14:44.92 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:14:44.98 AnalyticModule::Unknown in preston -- SPR @11:14:45.01 Carousel.seek 6 @11:14:45.42 Air.in :unlocking @11:14:45.61 Air.seek :clear @11:14:46.66 Lysis1.in :unlocking @11:14:46.80 Lysis1.seek :clear @11:14:47.42 Plunge.in :unlocking @11:14:47.52 Plunge.seek :clear @11:14:48.65 Carousel.seek 6 @11:14:48.92 Lysis1.in :fast @11:14:49.00 Lysis1.seek :locked @11:14:51.66 Plunge.in :default @11:14:51.79 Plunge.seek :locked @11:14:51.92 Air.in :fast @11:14:52.01 Air.seek :locked @11:14:53.02 Carousel.reconfigure :locked @11:14:53.21 Powering cartridge bus @11:14:56.87 Gate.setIR 1750,5000,0,6 @11:14:57.00 Gate.setIR 0,5000,0,6 @11:14:57.10 PuckHeater.configure :default @11:14:57.19 Gate.power :heat,:ON,limit to 5000mA @11:14:57.96 Mounted lysis Cartridge board#Q05031 @11:14:58.02 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @11:14:58.28 -> CCartridgesampler.source=nil @11:14:58.30 NameError in preston -- uninitialized constant CCartridgesampler @11:15:11.08 -> Cartridge.sampler.source=nil @11:15:17.47 -> slots {6 => { Type: :lyseHold_phyto, State: :dry}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @11:15:32.00 -> fire lyseHold_phyto # @11:15:33.02 Toroid.seek :clear @11:15:33.22 Carousel.in :default @11:15:33.36 Carousel.home.seek 6 @11:15:33.77 Carousel.reconfigure :locked @11:15:34.01 Gate.setIR 1750,5000,0,6 @11:15:34.14 Gate.setIR 0,5000,0,6 @11:15:34.24 PuckHeater.configure :default @11:15:34.34 Gate.power :heat,:ON,limit to 5000mA @11:15:35.04 Mounted lysis Cartridge board#Q05031 @11:15:35.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:15:35.18 Selecting Cartridge 6 @11:15:35.65 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @11:15:36.02 Preparing to filter cartridge 6 @11:15:36.42 Air.in :default @11:15:36.68 Air.seek :sealed @11:15:36.73 Priming sample loop w/150ml, bypass w/50ml @11:15:37.28 SamplePump.setPosition! 0ml @11:15:37.51 Exhaust.close @11:15:37.68 Bypass.open @11:15:38.89 upsync @11:15:39.23 Intake.open @11:15:40.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:15:42.65 Vacuum tare is 52% of full scale at 21C (drifted 1%) @11:15:42.83 Abs.in :default @11:15:42.91 Abs.hold @11:15:43.19 SamplePump.in :start @11:15:43.29 SamplePump.seek 50ml,3:50 @11:15:43.89 SamplePump.configure :prime @11:16:44.67 Abs.stop @11:16:44.76 SamplePump.setPosition! 0ml @11:16:44.84 Exhaust.open @11:16:46.24 Bypass.close @11:16:47.73 Abs.in :default @11:16:47.81 Abs.hold @11:16:48.18 SamplePump.in :start @11:16:48.28 SamplePump.seek 150ml,10:30 @11:16:48.87 SamplePump.configure :prime @11:19:51.60 Abs.stop @11:19:51.71 Bypass.open @11:19:52.36 Intake.close @11:19:53.12 Toroid.in :hold @11:19:53.21 Toroid.seek :crack @11:19:55.02 Toroid.seek :cartridge @11:19:56.18 Toroid.in :default @11:19:56.26 Toroid.seek :clear @11:19:58.36 Pressurizing cartridge to 26psi above 15.7psia @11:19:58.39 SamplePump.setPosition! 0ml @11:19:58.48 Intake.open @11:19:59.15 Bypass.close @11:19:59.79 Exhaust.close @11:20:00.60 Abs.in :default @11:20:00.69 Abs.hold @11:20:00.97 SamplePump.in :charge @11:20:01.07 SamplePump.seek 2.3ml,42 seconds @11:20:13.72 SamplePump.stop @11:20:13.83 Intake.close @11:20:14.11 Toroid.in :hold @11:20:14.23 Toroid.seek :bypass @11:20:15.93 Toroid.in :default @11:20:16.04 Toroid.seek :clear @11:20:17.72 Abs.stop @11:20:18.01 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only 0.2psi vacuum after 0.7ml @11:20:18.04 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @11:20:18.09 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only 0.2psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @11:20:20.17 upsync @11:43:23.19 -> PhytoSampler.primeVolume=PhytoSampler.bypassVolume=10 10 @11:43:31.16 -> Cartridge.unlock @11:43:31.23 Gate.power :heat,:OFF @11:43:31.25 Gate.power :cartridge,:OFF @11:43:31.37 Carousel.in :default @11:43:31.51 Carousel.home.seek 6 @11:43:31.96 Air.in :unlocking @11:43:32.12 Air.seek :clear @11:43:33.55 Lysis1.in :unlocking @11:43:33.69 Lysis1.seek :clear @11:43:33.83 Plunge.in :unlocking @11:43:33.93 Plunge.seek :clear @11:45:07.81 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @11:45:11.92 -> fire lyseHold_phyto @11:45:12.16 Selecting Cartridge 6 # @11:45:12.53 Carousel.seek 6 @11:45:12.80 Lysis1.in :fast @11:45:12.90 Lysis1.seek :locked @11:45:15.57 Plunge.in :default @11:45:15.67 Plunge.seek :locked @11:45:15.88 Air.in :fast @11:45:15.97 Air.seek :locked @11:45:16.98 Carousel.reconfigure :locked @11:45:17.17 Powering cartridge bus @11:45:19.82 Gate.setIR 1750,5000,0,6 @11:45:19.95 Gate.setIR 0,5000,0,6 @11:45:20.08 PuckHeater.configure :default @11:45:20.22 Gate.power :heat,:ON,limit to 5000mA @11:45:20.91 Mounted lysis Cartridge board#Q05031 @11:45:20.96 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:45:21.08 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @11:45:21.50 Preparing to filter cartridge 6 @11:45:22.30 Air.in :default @11:45:22.41 Bypass.open @11:45:22.57 Air.seek :sealed @11:45:22.84 Cmd::Error in detached from preston -- Cannot process while in PRIMING state @11:45:22.86 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @11:45:23.41 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @11:45:23.76 Toroid.in :hold @11:45:23.96 Toroid.seek :crack @11:45:24.42 upsync @11:45:25.39 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from preston" @11:45:25.99 Toroid.seek :cartridge @11:45:26.36 upsync @11:45:27.26 Toroid.in :default @11:45:27.44 Toroid.seek :clear @11:45:27.99 upsync @11:45:29.33 Exhaust.open @11:45:30.29 Pressurizing cartridge to 26psi above 15.6psia @11:45:30.31 SamplePump.setPosition! 0ml @11:45:30.41 Intake.open @11:45:31.09 Bypass.close @11:45:31.81 Exhaust.close @11:45:33.04 Abs.in :default @11:45:33.13 Abs.hold @11:45:33.45 SamplePump.seek 2.3ml,42 seconds @11:45:48.75 Abs.stop @11:45:48.85 Intake.close @11:45:49.60 Pressurized cartridge with 1.052ml to 26.9psi @11:45:49.62 Isolated @39.2psia (23.6psi) @11:45:49.66 Verifying cartridge holds at least 0.65..0.85 of 39.2psia @11:45:49.73 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:45:54.35 Cartridge pressure fell 4% to 37.8psia @11:45:54.48 Sampling 50.0ml @11:45:54.51 SamplePump.setPosition! 0ml @11:45:54.63 Exhaust.open @11:45:55.29 Intake.open @11:45:56.18 Abs.in :default @11:45:56.28 Abs.hold @11:45:56.63 SamplePump.in :start @11:45:56.73 SamplePump.seek 50ml,4:40 @11:45:57.32 SamplePump.configure :sample @11:45:59.73 Cartridge 6 filtering 2/50ml at 0.72ml/s with 17.6psi vacuum @11:46:59.79 Cartridge 6 filtering 39/50ml at 0.62ml/s with 13.8psi vacuum 5.5m deep (22.9psia) @11:47:18.23 Abs.stop @11:47:18.41 Intake.close @11:47:18.49 Exhaust.close @11:47:18.74 Toroid.in :hold @11:47:18.84 Toroid.seek :bypass @11:47:20.51 Toroid.in :default @11:47:20.61 Toroid.seek :clear @11:47:22.26 Sampled 50.0ml @11:47:22.72 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @11:47:23.26 Handoff.select :waste @11:47:23.46 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:47:25.61 upsync @11:47:30.10 Syringe tare is 52% of full scale at 21C (drifted 1%) @11:47:30.17 PinPort.in :locking @11:47:30.25 PinPort.seek :locked @11:47:30.76 Archive.in :fast @11:47:30.87 Lysis2.in :fast @11:47:30.92 Lysis1.seek 1350uL @11:47:31.11 Archive.seek :full @11:47:31.20 Lysis2.seek :empty @11:47:39.30 Plunge.seek :reagent @11:47:40.24 Evacuating puck with filter bubble point of 24psi @11:47:40.26 PinPort.seek :locked @11:47:40.42 Handoff.select :waste @11:47:40.45 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:47:47.07 Air syringe pumping up to 1 strokes to reach 4psi @11:47:47.09 Air stroke #1 of 1 @11:47:47.24 Air.in :pressurize @11:47:47.32 Air.seek :pressurized @11:47:52.96 2.3psi after pressurize @11:47:52.99 Air.in :compress @11:47:53.09 Air.seek :evac @11:47:56.17 1.9psi after compress @11:47:58.05 Reached 5.9 of 4.0psi after pumping 1594uL (1.00 strokes) @11:47:58.08 Air.in :default @11:47:58.21 Air.hold @11:47:58.37 Handoff.select :blocked @11:47:58.55 Overpressure in PROCESSING -- Waste port clogged (5.7psi) @11:47:58.57 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 4psi" @11:47:58.61 BadNews.email "Waste port clogged (5.7psi)",Subject: "Overpressure in PROCESSING" @11:48:02.06 upsync @11:53:03.11 -> Syringe.pis @11:53:03.16 NoMethodError in preston -- undefined method `pis' for Syringe:Class @11:53:06.62 -> Syringe.psi 8.10867463952752 @11:53:30.14 -> Handoff.waste @11:53:30.17 Handoff.select :waste Handoff Valve::Manifold selects waste @11:55:27.31 -> Thread[:PROCESSING].resume @11:55:27.38 Resuming Thread (suspended since 11:47:47) # @11:55:27.42 Air syringe pumping up to 1 strokes to reach 4psi @11:55:27.45 Air stroke #1 of 1 @11:55:27.64 Air.in :compress @11:55:27.73 Air.seek :evac @11:55:27.92 0.2psi after compress @11:55:38.51 0.2psi after compress (stable) @11:55:38.53 Air.in :default @11:55:38.64 Air.hold @11:55:38.88 at 0.2psi Opened to waste @11:55:38.90 Handoff.select :blocked @11:55:39.08 Archive syringe pumping up to 8 strokes to reach 24psi @11:55:39.10 Archive stroke #1 of 8 @11:55:39.24 Archive.in :pressurize @11:55:39.32 Archive.seek :pressurized @11:55:51.77 1.4psi after pressurize @11:56:18.36 8.2psi after pressurize (stable) @11:56:18.39 Archive.in :compress @11:56:18.49 Archive.seek :empty @11:56:34.23 13.0psi after compress @11:56:44.82 12.6psi after compress (stable) @11:56:44.85 Archive.in :gulp @11:56:44.95 Archive.seek :topVent @11:56:51.28 12.4psi after gulp @11:56:56.87 11.7psi after gulp (stable) @11:56:56.88 Archive stroke #2 of 8 @11:56:57.45 Archive.in :pressurize @11:56:57.55 Archive.seek :pressurized @11:57:10.00 12.5psi after pressurize @11:57:16.10 11.8psi after pressurize (stable) @11:57:16.15 Archive.in :compress @11:57:16.35 Archive.seek :empty @11:57:28.76 Reached 24.1 of 24.0psi after pumping 3388uL (1.96 strokes) @11:57:28.79 Archive.in :default @11:57:28.89 Archive.hold @11:57:29.01 Handoff.select :waste @11:57:29.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:57:39.27 delay 1:00 @11:58:39.30 Handoff.select :blocked @11:58:39.48 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:59:02.07 Handoff.select :waste @11:59:02.26 1.0psi at evacEnd @11:59:02.28 Handoff.select :blocked @11:59:02.46 Archive.in :fast @11:59:02.53 Archive.seek :topVent @11:59:09.56 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @11:59:16.17 Stable @0.6psi after Handoff blocked @11:59:16.20 Archive syringe pumping up to 8 strokes to reach 25psi @11:59:16.24 Archive stroke #1 of 8 @11:59:16.39 Archive.in :pressurize @11:59:16.48 Archive.seek :pressurized @11:59:28.94 1.6psi after pressurize @11:59:28.97 Archive.in :compress @11:59:29.09 Archive.seek :empty @11:59:44.82 14.5psi after compress @11:59:55.41 13.3psi after compress (stable) @11:59:55.44 Archive.in :gulp @11:59:55.57 Archive.seek :topVent @12:00:01.90 14.3psi after gulp @12:00:07.49 13.7psi after gulp (stable) @12:00:07.51 Archive stroke #2 of 8 @12:00:07.66 Archive.in :pressurize @12:00:07.75 Archive.seek :pressurized @12:00:20.21 14.1psi after pressurize @12:00:20.24 Archive.in :compress @12:00:20.37 Archive.seek :empty @12:00:32.31 Reached 24.4 of 24.6psi after pumping 3376uL (1.95 strokes) @12:00:32.35 Archive.in :default @12:00:32.45 Archive.hold @12:00:32.55 Handoff.select :waste @12:00:32.73 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:00:39.82 delay 1:00 @12:01:39.85 Handoff.select :blocked @12:01:40.02 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:02:02.61 Handoff.select :waste @12:02:02.77 0.7psi at evacEnd @12:02:02.80 Air.seek :fullVent @12:02:04.38 Archive.in :fast @12:02:04.48 Archive.seek :topVent @12:02:11.37 Seawater evacuated, Pressure above/below filter released. @12:02:11.49 Lysis1.in :slow @12:02:11.63 Lysis1.seek 1050uL @12:02:41.29 delay 5 seconds @12:02:46.31 Handoff.select :blocked @12:02:46.55 Lysis1.seek 450uL @12:03:45.68 delay 3 seconds @12:03:48.77 Air.in mix with 1.26318 parts Lysis1 @12:03:49.56 Air.seek :evac @12:03:49.66 Lysis1.seek 300uL @12:04:04.60 Lysis1.seek :empty @12:04:34.31 Lysis buffer delivered @18.0psi @12:04:34.33 Polling Can every 2:00 @12:04:34.92 PuckHeater.configure :default #ambient 21.1C @12:04:35.00 PuckHeater.seek 79.8C @12:08:57.32 PuckHeater.hold 2:00,79.8C @12:10:57.22 Polling Can every 2:00 @12:10:57.77 PuckHeater.configure :default #ambient 21.1C @12:10:57.87 PuckHeater.hold 30 seconds,78.7C @12:11:28.02 Polling Can every 2:00 @12:11:28.57 PuckHeater.configure :default #ambient 21.1C @12:11:28.66 PuckHeater.hold 30 seconds,78.2C @12:11:58.82 Polling Can every 2:00 @12:11:59.37 PuckHeater.configure :default #ambient 21.1C @12:11:59.46 PuckHeater.hold 10:00,77.7C @12:21:58.26 Lysis complete. Syringe pressure @18.2psi @12:21:58.29 Archive syringe pumping up to 5 strokes to reach 40psi @12:21:58.33 Archive stroke #1 of 5 @12:21:58.45 Archive.in :pressurize @12:21:58.53 Archive.seek :pressurized @12:22:11.48 18.0psi after pressurize @12:22:17.08 17.5psi after pressurize (stable) @12:22:17.11 Archive.in :compress @12:22:17.21 Archive.seek :empty @12:22:26.79 Reached 40.0 of 40.0psi after pumping 1596uL (0.92 strokes) @12:22:26.81 Archive.in :default @12:22:26.91 Archive.hold @12:22:27.03 delay 2 seconds @12:22:29.06 Archive.in :fast @12:22:29.14 Archive.seek :full @12:22:35.91 Lysis1.seek 16uL @12:22:37.61 delay 1 second @12:22:38.65 Archive.seek :full @12:22:38.69 Lysis1.seek :empty @12:22:40.46 Handoff.select :blocked @12:22:40.65 Initial air pressure @48.7psi @12:22:40.74 Lysis1.in :extract @12:22:40.88 Lysis1.seek 600uL,nil @12:22:43.96 Archive syringe pumping up to 6 strokes to reach 25psi @12:22:43.98 Archive stroke #1 of 6 @12:22:44.16 Archive.in :pressurize @12:22:44.25 Archive.seek :pressurized @12:22:47.23 Archive.in :default @12:22:47.32 Archive.hold @12:22:47.73 Initial 600uL lysate @17.6psi @12:22:47.75 Handoff.select :blocked @12:22:47.92 Initial air pressure @16.9psi @12:22:48.02 Lysis1.seek 825uL,nil @12:22:49.39 Archive syringe pumping up to 6 strokes to reach 17psi @12:22:49.41 Archive stroke #1 of 6 @12:22:49.56 Archive.in :pressurize @12:22:50.24 Archive.seek :pressurized @12:22:58.58 Archive.in :default @12:22:58.66 Archive.hold @12:22:59.10 Recovered 825uL lysate @15.7psi @12:22:59.13 Air.in :halfSpeed @12:22:59.24 Air.seek :fullVent @12:23:01.69 Puck Top Vented, bottom @11.4psi @12:23:01.71 Handoff.select :waste @12:23:01.89 delay 5 seconds @12:23:06.97 Handoff open to waste, bottom @0.0psi @12:23:07.00 Air.in :default @12:23:07.10 Air.seek :evac @12:23:08.79 Archive.stop @12:23:08.96 Handoff.select :close @12:23:09.14 Gate.power :heat,:OFF @12:23:09.16 Gate.power :cartridge,:OFF @12:23:09.29 PinPort.in :unlocking @12:23:09.37 PinPort.seek :clear @12:23:09.87 Carousel.in :default @12:23:10.02 Carousel.home.seek 6 @12:23:10.39 Plunge.seek :locked @12:23:11.32 Archive.in :unlocking @12:23:11.48 Archive.seek :clear @12:23:11.61 Air.in :unlocking @12:23:11.77 Air.seek :clear @12:23:11.86 Lysis1.in :unlocking @12:23:12.04 Lysis1.seek :clear @12:23:12.22 Lysis2.in :unlocking @12:23:12.32 Lysis2.seek :clear @12:23:16.96 Gate.power :analytic1,:OFF @12:23:16.98 Gate.power :motor,:OFF @12:23:17.05 Gate.power :core,:OFF @12:23:17.08 Gate.power :main,:OFF @12:23:17.11 Safely stopped and ready to power off @12:23:18.84 -> ESP.reay! @12:23:18.89 NoMethodError in preston -- undefined method `reay!' for ESP:Module @12:23:22.02 -> ESP.ready! @12:23:22.12 Gate.power :sampler,:OFF,limit to 3500mA @12:23:22.14 Gate.power :skimmer,:OFF,limit to 3500mA @12:23:22.22 Gate.power :main,:ON,limit to 5000mA @12:23:22.24 Gate.power :analytic1,:ON,limit to 4000mA @12:23:22.35 Powering core bus @12:23:25.76 Plunge.configure :default @12:23:25.88 SamplePump.configure :default @12:23:25.99 Toroid.configure :default @12:23:26.36 Carousel.configure :default @12:23:26.47 Abs.configure :default @12:23:26.58 PhaseSeparator.configure :default @12:23:26.69 Archive.configure :default @12:23:26.80 Air.configure :default @12:23:26.88 Lysis1.configure :default @12:23:26.97 Lysis2.configure :default @12:23:27.07 PinPort.configure :default @12:23:27.17 All elf controllers running firmware version 4.92 @12:23:27.20 Gate.power :motor,:ON,limit to 5000mA @12:23:29.61 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:23:29.64 AnalyticModule::Unknown in preston -- SPR @12:23:30.15 Toroid.home.jog 0 @12:23:30.26 Plunge.home.jog 0 @12:23:30.88 Lysis2.home.jog 0 @12:23:30.91 Air.home.jog 0 @12:23:30.98 Carousel.home.jog 0 @12:23:31.03 PinPort.home.jog 0 @12:23:31.08 Lysis1.home.jog 0 @12:23:31.12 Archive.home.jog 0 @12:23:31.81 Toroid.in :homing @12:23:31.89 Plunge.in :homing @12:23:32.25 Toroid.jog 630 @12:23:32.33 Plunge.jog 1610 @12:23:32.62 Lysis2.in :homing @12:23:32.69 Lysis1.in :homing @12:23:32.73 Archive.in :homing @12:23:32.77 PinPort.in :homing @12:23:32.82 Air.in :homing @12:23:33.09 PinPort.jog -104 @12:23:33.14 Lysis1.jog 1394 @12:23:33.21 Archive.jog 1403 @12:23:33.32 Lysis2.jog 1406 @12:23:33.42 Air.jog 1374 @12:23:33.73 PinPort.setRawPosition! 0 @12:23:33.75 Plunge.setRawPosition! 50 @12:23:33.91 PinPort.seek :home @12:23:33.93 Plunge.seek :home @12:23:34.18 PinPort.in :default @12:23:34.36 Plunge.in :default @12:23:34.55 Toroid.setRawPosition! 0 @12:23:34.64 Toroid.seek :home @12:23:34.81 Lysis2.setRawPosition! 50 @12:23:34.86 Lysis1.setRawPosition! 50 @12:23:34.91 Air.setRawPosition! 50 @12:23:34.95 Archive.setRawPosition! 50 @12:23:34.99 Toroid.in :default @12:23:35.26 Lysis1.seek :home @12:23:35.32 Air.seek :home @12:23:35.40 Lysis2.seek :home @12:23:35.52 Archive.seek :home @12:23:35.78 Lysis1.in :default @12:23:35.94 Lysis2.in :default @12:23:35.98 Air.in :default @12:23:36.07 Archive.in :default @12:23:36.53 Toroid.seek :clear @12:23:38.21 Carousel.in :default @12:23:38.36 Carousel.home.seek 6 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 6 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:23:41.83 -> Cartridge.lock @12:23:42.02 Lysis1.in :fast @12:23:42.12 Lysis1.seek :locked @12:23:44.79 Plunge.seek :locked @12:23:44.99 Air.in :fast @12:23:45.07 Air.seek :locked @12:23:46.07 Carousel.reconfigure :locked @12:23:46.29 Powering cartridge bus @12:23:48.96 Gate.setIR 1750,5000,0,6 @12:23:49.10 Gate.setIR 0,5000,0,6 @12:23:49.19 PuckHeater.configure :default #ambient 21.1C @12:23:49.28 Gate.power :heat,:ON,limit to 5000mA @12:23:49.98 Mounted lysis Cartridge board#Q05031 @12:23:50.04 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:24:22.54 -> Cartride.sampler.source @12:24:22.59 NameError in preston -- uninitialized constant Cartride @12:24:28.47 -> Cartridge.sampler.source @12:24:31.92 -> Cartridge.sampler.source @12:24:49.58 -> PhytoSampler.filter 100 @12:24:49.83 Air.in :default @12:24:49.97 Air.seek :sealed @12:24:50.09 Priming sample loop w/10ml, bypass w/10ml @12:24:50.14 SamplePump.setPosition! 0ml @12:24:50.26 Bypass.open @12:24:51.71 Intake.open @12:24:52.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:24:55.10 Vacuum tare is 53% of full scale at 21C (drifted 1%) @12:24:55.27 Abs.in :default @12:24:55.35 Abs.hold @12:24:55.62 SamplePump.in :start @12:24:55.73 SamplePump.seek 10ml,1:10 @12:24:56.31 SamplePump.configure :prime @12:25:08.29 Abs.stop @12:25:08.38 SamplePump.setPosition! 0ml @12:25:08.46 Exhaust.open @12:25:09.79 Bypass.close @12:25:11.74 Abs.in :default @12:25:11.91 Abs.hold @12:25:12.30 SamplePump.in :start @12:25:12.41 SamplePump.seek 10ml,1:10 @12:25:12.99 SamplePump.configure :prime @12:25:24.99 Abs.stop @12:25:25.15 Bypass.open @12:25:25.84 Intake.close @12:25:26.63 Toroid.in :hold @12:25:26.72 Toroid.seek :crack @12:25:28.30 Toroid.seek :cartridge @12:25:29.45 Toroid.in :default @12:25:29.54 Toroid.seek :clear @12:25:31.62 Pressurizing cartridge to 26psi above 15.6psia @12:25:31.64 SamplePump.setPosition! 0ml @12:25:31.73 Intake.open @12:25:32.41 Bypass.close @12:25:33.11 Exhaust.close @12:25:33.91 Abs.in :default @12:25:33.99 Abs.hold @12:25:34.28 SamplePump.in :charge @12:25:34.38 SamplePump.seek 2.3ml,42 seconds @12:25:48.43 Abs.stop @12:25:48.52 Intake.close @12:25:49.30 Pressurized cartridge with 0.887ml to 26.6psi @12:25:49.32 Isolated @31.2psia (15.6psi) @12:25:49.36 Verifying cartridge holds at least 0.65..0.85 of 31.2psia @12:25:49.44 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:25:55.05 Cartridge pressure fell 24% to 23.8psia @12:25:55.36 Toroid.in :hold @12:25:55.46 Toroid.seek :bypass @12:25:57.35 Toroid.in :default @12:25:57.44 Toroid.seek :clear @12:25:59.09 Cartridge::Sampler::PossibleLeak in preston -- Retained only 23.8psia @12:28:33.42 -> PhytoSampler.filter 100 @12:28:34.22 Air.seek :sealed @12:28:34.44 Bypass.open @12:28:35.23 Toroid.in :hold @12:28:35.32 Toroid.seek :crack @12:28:36.90 Toroid.seek :cartridge @12:28:38.04 Toroid.in :default @12:28:38.17 Toroid.seek :clear @12:28:40.00 Exhaust.open @12:28:40.90 Pressurizing cartridge to 26psi above 15.5psia @12:28:40.92 SamplePump.setPosition! 0ml @12:28:41.02 Intake.open @12:28:41.74 Bypass.close @12:28:42.40 Exhaust.close @12:28:43.19 Abs.in :default @12:28:43.26 Abs.hold @12:28:43.52 SamplePump.seek 2.3ml,42 seconds @12:28:59.34 Intake.close @12:28:59.63 Toroid.in :hold @12:28:59.73 Toroid.seek :bypass @12:29:01.42 Toroid.in :default @12:29:01.52 Toroid.seek :clear @12:29:03.17 Abs.stop @12:29:03.36 UserAbort in preston -- User Abort!! @12:29:07.24 -> Cartridge.unlock @12:29:07.31 Gate.power :heat,:OFF @12:29:07.33 Gate.power :cartridge,:OFF @12:29:07.45 Carousel.in :default @12:29:07.59 Carousel.home.seek 6 @12:29:08.02 Air.in :unlocking @12:29:08.20 Air.seek :clear @12:29:09.62 Lysis1.in :unlocking @12:29:09.78 Lysis1.seek :clear @12:29:09.88 Plunge.in :unlocking @12:29:09.99 Plunge.seek :clear @12:30:26.07 -> to 6 @12:30:26.42 Carousel.seek 6 @12:30:26.69 Lysis1.in :fast @12:30:26.78 Lysis1.seek :locked @12:30:29.90 Plunge.in :default @12:30:30.14 Plunge.seek :locked @12:30:30.36 Air.in :fast @12:30:30.45 Air.seek :locked @12:30:31.45 Carousel.reconfigure :locked @12:30:31.64 Powering cartridge bus @12:30:34.33 Gate.setIR 1750,5000,0,6 @12:30:34.46 Gate.setIR 0,5000,0,6 @12:30:34.57 PuckHeater.configure :default #ambient 21.1C @12:30:34.68 Gate.power :heat,:ON,limit to 5000mA @12:30:35.39 Mounted lysis Cartridge board#Q05092 @12:30:35.45 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:30:38.33 -> PhytoSampler.filter 100 @12:30:38.62 Air.in :default @12:30:38.77 Air.seek :sealed @12:30:38.89 Bypass.open @12:30:39.64 Toroid.in :hold @12:30:39.73 Toroid.seek :crack @12:30:41.31 Toroid.seek :cartridge @12:30:42.45 Toroid.in :default @12:30:42.54 Toroid.seek :clear @12:30:44.37 Exhaust.open @12:30:45.24 Pressurizing cartridge to 26psi above 15.5psia @12:30:45.27 SamplePump.setPosition! 0ml @12:30:45.37 Intake.open @12:30:46.04 Bypass.close @12:30:46.73 Exhaust.close @12:30:47.53 Abs.in :default @12:30:47.63 Abs.hold @12:30:47.89 SamplePump.seek 2.3ml,42 seconds @12:31:02.13 Abs.stop @12:31:02.23 Intake.close @12:31:02.96 Pressurized cartridge with 0.911ml to 26.7psi @12:31:02.97 Isolated @38.4psia (22.9psi) @12:31:03.00 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @12:31:03.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:31:07.70 Cartridge pressure fell 1% to 38.0psia @12:31:07.84 Sampling 100.0ml @12:31:07.86 SamplePump.setPosition! 0ml @12:31:08.55 Exhaust.open @12:31:09.25 Intake.open @12:31:10.23 Abs.in :default @12:31:10.33 Abs.hold @12:31:10.67 SamplePump.in :start @12:31:10.77 SamplePump.seek 100ml,8:50 @12:31:11.36 SamplePump.configure :sample @12:31:13.77 Cartridge 6 filtering 2/100ml at 0.45ml/s with 36.1psi vacuum @12:31:52.89 SamplePump.hold @12:31:53.14 Intake.close @12:31:53.25 Exhaust.close @12:31:53.53 Toroid.in :hold @12:31:53.63 Toroid.seek :bypass @12:31:55.32 Toroid.in :default @12:31:55.43 Toroid.seek :clear @12:31:57.08 Abs.stop @12:31:57.55 UserAbort in preston -- Sample Pump (SP) seek to 100ml Aborted! @12:32:05.71 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @12:32:11.56 -> fire lyseHold_phyto # @12:32:12.58 Toroid.seek :clear @12:32:12.79 Carousel.in :default @12:32:12.93 Carousel.home.seek 6 @12:32:13.34 Carousel.reconfigure :locked @12:32:13.58 Gate.setIR 1750,5000,0,6 @12:32:13.71 Gate.setIR 0,5000,0,6 @12:32:13.81 PuckHeater.configure :default #ambient 21.1C @12:32:13.92 Gate.power :heat,:ON,limit to 5000mA @12:32:16.61 Mounted lysis Cartridge board#Q05092 @12:32:16.66 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:32:16.73 Selecting Cartridge 6 @12:32:17.13 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @12:32:18.14 Preparing to filter cartridge 6 @12:32:18.50 Air.seek :sealed @12:32:18.82 Priming sample loop w/10ml, bypass w/10ml @12:32:18.85 SamplePump.setPosition! 0ml @12:32:19.01 Bypass.open @12:32:20.66 Intake.open @12:32:21.05 upsync @12:32:21.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:32:24.16 Vacuum tare is 52% of full scale at 21C (drifted 1%) @12:32:24.36 Abs.in :default @12:32:24.44 Abs.hold @12:32:24.71 SamplePump.in :start @12:32:24.82 SamplePump.seek 10ml,1:10 @12:32:25.40 SamplePump.configure :prime @12:32:37.40 Abs.stop @12:32:37.49 SamplePump.setPosition! 0ml @12:32:37.57 Exhaust.open @12:32:38.98 Bypass.close @12:32:40.51 Abs.in :default @12:32:40.62 Abs.hold @12:32:40.96 SamplePump.in :start @12:32:41.07 SamplePump.seek 10ml,1:10 @12:32:41.65 SamplePump.configure :prime @12:32:53.63 Abs.stop @12:32:53.74 Bypass.open @12:32:54.43 Intake.close @12:32:55.24 Toroid.in :hold @12:32:55.33 Toroid.seek :crack @12:32:56.91 Toroid.seek :cartridge @12:32:58.05 Toroid.in :default @12:32:58.17 Toroid.seek :clear @12:33:00.89 Pressurizing cartridge to 26psi above 15.6psia @12:33:00.91 SamplePump.setPosition! 0ml @12:33:01.02 Intake.open @12:33:01.69 Bypass.close @12:33:02.37 Exhaust.close @12:33:03.15 Abs.in :default @12:33:03.23 Abs.hold @12:33:03.49 SamplePump.in :charge @12:33:03.58 SamplePump.seek 2.3ml,42 seconds @12:33:14.70 Abs.stop @12:33:14.81 Intake.close @12:33:15.55 Pressurized cartridge with 0.552ml to 27.7psi @12:33:15.56 Isolated @38.8psia (23.2psi) @12:33:15.59 Verifying cartridge holds at least 0.65..0.85 of 38.8psia @12:33:15.68 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:33:20.31 Cartridge pressure fell 1% to 38.5psia @12:33:20.44 Sampling 50.0ml @12:33:20.46 SamplePump.setPosition! 0ml @12:33:20.56 Exhaust.open @12:33:21.21 Intake.open @12:33:22.07 Abs.in :default @12:33:22.21 Abs.hold @12:33:22.56 SamplePump.in :start @12:33:22.66 SamplePump.seek 50ml,4:40 @12:33:23.25 SamplePump.configure :sample @12:33:25.66 Cartridge 6 filtering 2/50ml at 0.63ml/s with 13.7psi vacuum 6.2m deep (23.8psia) @12:34:25.72 Cartridge 6 filtering 39/50ml at 0.62ml/s with 12.5psi vacuum 7.8m deep (26.2psia) @12:34:44.01 Abs.stop @12:34:44.23 Intake.close @12:34:44.32 Exhaust.close @12:34:44.57 Toroid.in :hold @12:34:44.67 Toroid.seek :bypass @12:34:46.34 Toroid.in :default @12:34:46.44 Toroid.seek :clear @12:34:48.11 Sampled 50.0ml @12:34:49.09 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @12:34:49.73 Handoff.select :waste @12:34:49.94 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:34:51.86 upsync @12:34:56.59 Syringe tare is 52% of full scale at 21C (drifted 1%) @12:34:56.63 PinPort.in :locking @12:34:56.73 PinPort.seek :locked @12:34:57.24 Archive.in :fast @12:34:57.29 Lysis2.in :fast @12:34:57.39 Lysis1.seek 1350uL @12:34:57.63 Archive.seek :full @12:34:57.65 Lysis2.seek :empty @12:35:05.79 Plunge.seek :reagent @12:35:06.73 Evacuating puck with filter bubble point of 24psi @12:35:06.77 PinPort.seek :locked @12:35:06.92 Handoff.select :waste @12:35:06.96 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:35:13.58 Air syringe pumping up to 1 strokes to reach 4psi @12:35:13.60 Air stroke #1 of 1 @12:35:13.75 Air.in :pressurize @12:35:13.83 Air.seek :pressurized @12:35:14.99 -> Syringe.psi 0.57108338449865 @12:35:17.71 -> Syringe.psi 0.660943295931162 @12:35:19.52 1.1psi after pressurize @12:35:19.55 Air.in :compress @12:35:19.64 Air.seek :evac @12:35:20.29 -> Syringe.psi 2.0183009054642 @12:35:22.81 Reached 3.4 of 4.0psi after pumping 1564uL (0.98 strokes) @12:35:22.84 Air.in :default @12:35:22.97 Air.hold @12:35:23.16 Handoff.select :blocked @12:35:23.34 Overpressure in PROCESSING -- Waste port clogged (0.7psi) @12:35:23.36 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 166uL (sensed 163uL)" @12:35:23.41 BadNews.email "Waste port clogged (0.7psi)",Subject: "Overpressure in PROCESSING" @12:35:23.54 -> Syringe.psi 2.58583718819577 @12:35:25.75 upsync @12:35:36.05 -> Syringe.psi 4.86071178814487 @12:36:38.22 -> Handoff.waste @12:36:38.25 Handoff.select :waste Handoff Valve::Manifold selects waste @12:37:04.29 -> Cartridge.unlock @12:37:04.35 Gate.power :heat,:OFF @12:37:04.37 Gate.power :cartridge,:OFF @12:37:04.52 PinPort.in :unlocking @12:37:04.61 PinPort.seek :clear @12:37:05.10 Carousel.in :default @12:37:05.25 Carousel.home.seek 6 @12:37:05.62 Archive.in :unlocking @12:37:05.76 Archive.seek :clear @12:37:05.94 Air.in :unlocking @12:37:06.15 Air.seek :clear @12:37:06.21 Lysis2.in :unlocking @12:37:06.36 Lysis2.seek :clear @12:37:10.94 Lysis1.in :unlocking @12:37:11.10 Lysis1.seek :clear @12:37:11.23 Plunge.in :unlocking @12:37:11.32 Plunge.seek :clear @12:37:47.27 -> Cartridge.lock @12:37:47.47 Lysis1.in :fast @12:37:47.55 Lysis1.seek :locked @12:37:50.24 Plunge.in :default @12:37:50.32 Plunge.seek :locked @12:37:50.51 Air.in :fast @12:37:50.60 Air.seek :locked @12:37:51.60 Carousel.reconfigure :locked @12:37:51.80 Powering cartridge bus @12:37:54.50 Gate.setIR 1750,5000,0,6 @12:37:54.62 Gate.setIR 0,5000,0,6 @12:37:54.72 PuckHeater.configure :default #ambient 21.1C @12:37:54.84 Gate.power :heat,:ON,limit to 5000mA @12:37:56.15 Mounted lysis Cartridge board#Q05092 @12:37:56.30 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:37:57.71 -> PP.cartridge @12:37:57.75 PinPort.in :locking @12:37:57.87 PinPort.seek :locked Handoff Pin Port (PP) cartridge @12:40:26.39 -> MainThread.exit # @12:40:26.57 Handoff.select :close @12:40:26.82 Session Ended @12:57:55.01PDT17-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:57:56.75 >BREAK< ESP3 Gateway v1.43 for core bus @12:57:56.77 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:58:00.33 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @12:58:01.00 Sea pressure (Abs) tare is 6% of full scale @12:58:05.10 Polling Can every 2:00 @12:58:05.15 Waiting for espclients... @12:58:05.36 Session Begun @12:58:58.60 -> PhytoSampler.bypassVolume=PhytoSampler.primeVolume=10 10 @12:59:01.66 -> to 6 @12:59:01.79 Gate.power :heat,:OFF @12:59:01.90 PinPort.configure :unlocking @12:59:02.04 PinPort.seek :clear @12:59:02.56 Carousel.configure :default @12:59:02.87 Gate.power :sampler,:OFF,limit to 3500mA @12:59:02.90 Gate.power :skimmer,:OFF,limit to 3500mA @12:59:02.94 Gate.power :analytic1,:ON,limit to 4000mA @12:59:03.15 Gate.power :cartridge,:OFF @12:59:03.75 Plunge.configure :default @12:59:03.85 Plunge.hold @12:59:03.97 Plunge.configure :default @12:59:04.18 SamplePump.configure :default @12:59:04.38 Toroid.configure :default @12:59:04.47 Toroid.hold @12:59:04.59 Toroid.configure :default @12:59:04.78 Carousel.hold @12:59:04.91 Carousel.configure :default @12:59:05.36 Abs.configure :default @12:59:05.55 PhaseSeparator.configure :default @12:59:05.74 Archive.configure :default @12:59:05.84 Archive.hold @12:59:05.96 Archive.configure :default @12:59:06.18 Air.configure :default @12:59:06.27 Air.hold @12:59:06.40 Air.configure :default @12:59:06.55 Lysis1.configure :default @12:59:06.65 Lysis1.hold @12:59:07.03 Lysis1.configure :default @12:59:07.61 Lysis2.configure :default @12:59:07.71 Lysis2.hold @12:59:07.81 Lysis2.configure :default @12:59:08.02 PinPort.in :default @12:59:08.15 PinPort.hold @12:59:08.27 PinPort.configure :default @12:59:08.37 All elf controllers running firmware version 4.92 @12:59:08.53 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:59:08.58 AnalyticModule::Unknown in preston -- SPR @12:59:08.61 Carousel.seek 6 @12:59:09.00 Air.in :unlocking @12:59:09.15 Air.seek :clear @12:59:10.23 Lysis1.in :unlocking @12:59:10.39 Lysis1.seek :clear @12:59:10.48 Plunge.in :unlocking @12:59:10.60 Plunge.seek :clear @12:59:11.84 Carousel.seek 6 @12:59:12.16 Lysis1.in :fast @12:59:12.25 Lysis1.seek :locked @12:59:14.90 Plunge.in :default @12:59:15.02 Plunge.seek :locked @12:59:15.16 Air.in :fast @12:59:15.24 Air.seek :locked @12:59:16.25 Carousel.reconfigure :locked @12:59:16.42 Powering cartridge bus @12:59:20.10 Gate.setIR 1750,5000,0,6 @12:59:20.26 Gate.setIR 0,5000,0,6 @12:59:20.36 PuckHeater.configure :default @12:59:20.45 Gate.power :heat,:ON,limit to 5000mA @12:59:23.19 Mounted lysis Cartridge board#Q05092 @12:59:23.25 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:59:45.47 -> Cartridge.sampler.source=nil @12:59:47.91 -> slots {6 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:00:14.99 -> slot 6=> [:dry, lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:00:33.94 -> #run with PP change. First burkert only @13:00:39.85 -> fire lyseHold_phyto # @13:00:40.86 Toroid.seek :clear @13:00:41.07 Carousel.in :default @13:00:41.22 Carousel.home.seek 6 @13:00:41.65 Carousel.reconfigure :locked @13:00:41.90 Gate.setIR 1750,5000,0,6 @13:00:42.04 Gate.setIR 0,5000,0,6 @13:00:42.17 PuckHeater.configure :default @13:00:42.26 Gate.power :heat,:ON,limit to 5000mA @13:00:43.34 Mounted lysis Cartridge board#Q05092 @13:00:43.40 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:00:43.56 Selecting Cartridge 6 @13:00:43.97 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:00:44.45 Preparing to filter cartridge 6 @13:00:44.84 Air.in :default @13:00:45.06 Air.seek :sealed @13:00:45.14 Priming sample loop w/10ml, bypass w/10ml @13:00:45.22 SamplePump.setPosition! 0ml @13:00:45.37 Exhaust.close @13:00:45.57 Bypass.open @13:00:46.85 upsync @13:00:47.08 Intake.open @13:00:47.89 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:00:50.52 Vacuum tare is 52% of full scale at 21C (drifted 1%) @13:00:50.71 Abs.in :default @13:00:50.79 Abs.hold @13:00:51.07 SamplePump.in :start @13:00:51.17 SamplePump.seek 10ml,1:10 @13:00:51.76 SamplePump.configure :prime @13:01:03.72 Abs.stop @13:01:03.81 SamplePump.setPosition! 0ml @13:01:03.89 Exhaust.open @13:01:05.29 Bypass.close @13:01:06.79 Abs.in :default @13:01:06.89 Abs.hold @13:01:07.23 SamplePump.in :start @13:01:07.34 SamplePump.seek 10ml,1:10 @13:01:07.92 SamplePump.configure :prime @13:01:19.92 Abs.stop @13:01:20.04 Bypass.open @13:01:20.73 Intake.close @13:01:21.52 Toroid.in :hold @13:01:22.04 Toroid.seek :crack @13:01:23.73 Toroid.seek :cartridge @13:01:24.88 Toroid.in :default @13:01:24.97 Toroid.seek :clear @13:01:27.06 Pressurizing cartridge to 26psi above 15.6psia @13:01:27.08 SamplePump.setPosition! 0ml @13:01:27.19 Intake.open @13:01:27.90 Bypass.close @13:01:28.56 Exhaust.close @13:01:29.33 Abs.in :default @13:01:29.42 Abs.hold @13:01:29.67 SamplePump.in :charge @13:01:29.78 SamplePump.seek 2.3ml,42 seconds @13:01:42.97 Abs.stop @13:01:43.09 Intake.close @13:01:43.86 Pressurized cartridge with 0.779ml to 26.8psi @13:01:43.88 Isolated @38.1psia (22.5psi) @13:01:43.92 Verifying cartridge holds at least 0.65..0.85 of 38.1psia @13:01:43.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:01:48.60 Cartridge pressure fell 6% to 35.9psia @13:01:48.74 Sampling 50.0ml @13:01:48.76 SamplePump.setPosition! 0ml @13:01:48.86 Exhaust.open @13:01:49.50 Intake.open @13:01:50.43 Abs.in :default @13:01:50.52 Abs.hold @13:01:50.88 SamplePump.in :start @13:01:50.98 SamplePump.seek 50ml,4:40 @13:01:51.57 SamplePump.configure :sample @13:01:53.99 Cartridge 6 filtering 2/50ml at 0.45ml/s with 20.8psi vacuum @13:02:54.04 Cartridge 6 filtering 39/50ml at 0.61ml/s with 22.5psi vacuum @13:03:12.66 Abs.stop @13:03:12.85 Intake.close @13:03:12.95 Exhaust.close @13:03:13.17 Toroid.in :hold @13:03:13.26 Toroid.seek :bypass @13:03:14.93 Toroid.in :default @13:03:15.03 Toroid.seek :clear @13:03:16.69 Sampled 50.0ml @13:03:17.54 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @13:03:18.31 Handoff.select :waste @13:03:18.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:03:20.22 upsync @13:03:25.12 Syringe tare is 52% of full scale at 21C (drifted 1%) @13:03:25.15 PinPort.in :locking @13:03:25.27 PinPort.seek :locked @13:03:25.78 Archive.in :fast @13:03:25.85 Lysis2.in :fast @13:03:25.92 Lysis1.seek 1350uL @13:03:26.12 Archive.seek :full @13:03:26.23 Lysis2.seek :empty @13:03:34.36 Plunge.seek :reagent @13:03:35.30 Evacuating puck with filter bubble point of 24psi @13:03:35.33 PinPort.seek :locked @13:03:35.48 Handoff.select :waste @13:03:35.52 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:03:37.73 -> Syringe.psi 0.189178760910522 @13:03:40.24 -> Syringe.psi 0.0378357521821044 @13:03:42.20 Air syringe pumping up to 1 strokes to reach 4psi @13:03:42.22 -> Syringe.psi @13:03:42.28 Air stroke #1 of 1 0.0662125663186828 @13:03:42.52 Air.in :pressurize @13:03:42.63 Air.seek :pressurized @13:03:45.12 -> Syringe.psi 0.65266672514133 @13:03:47.63 -> Syringe.psi 0.77563291973317 @13:03:48.30 0.9psi after pressurize @13:03:48.33 Air.in :compress @13:03:48.44 Air.seek :evac @13:03:50.14 Reached 5.5 of 4.0psi after pumping 1512uL (0.95 strokes) @13:03:50.18 -> Syringe.psi @13:03:50.63 Air.in :default 0.704690884391709 @13:03:50.89 Air.hold @13:03:51.10 Handoff.select :blocked @13:03:51.36 Overpressure in PROCESSING -- Waste port clogged (0.7psi) @13:03:51.39 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 218uL (sensed 215uL)" @13:03:51.43 BadNews.email "Waste port clogged (0.7psi)",Subject: "Overpressure in PROCESSING" @13:03:53.50 upsync @13:03:55.83 -> Syringe.psi 3.97275397912108 @13:03:58.97 -> Syringe.psi 4.30381681071452 @13:04:08.29 -> Syringe.psi 4.58758495208031 @13:04:12.61 -> Syringe.psi 4.7436574298315 @13:04:17.57 -> Syirnge.psi @13:04:17.61 NameError in preston -- uninitialized constant Syirnge @13:04:22.81 -> Syringe.psi 4.71528061569492 @13:04:33.34 -> Syringe.psi 4.84770574833229 @13:04:34.17 -> Syringe.psi 4.84770574833229 @13:04:34.87 -> Syringe.psi 4.82405840321846 @13:04:35.47 -> Syringe.psi 4.83351734126398 @13:04:36.11 -> Syringe.psi 4.85243521735504 @13:04:37.10 -> Syringe.psi 4.84770574833229 @13:05:11.13 -> Syringe.psi 4.73892796080872 @13:05:14.16 -> Syringe.psi 4.80041105810466 @13:05:15.53 -> Syringe.psi 4.87608256246887 @13:06:22.71 -> Syringe.psi 0.903328583347786 @13:06:31.10 -> Cartridge.lock @13:06:31.17 Gate.power :heat,:OFF @13:06:31.19 Gate.power :cartridge,:OFF @13:06:32.77 Powering cartridge bus @13:06:35.43 Gate.setIR 1750,5000,0,6 @13:06:35.56 Gate.setIR 0,5000,0,6 @13:06:35.65 PuckHeater.configure :default @13:06:35.75 Gate.power :heat,:ON,limit to 5000mA @13:06:36.46 Mounted lysis Cartridge board#Q05092 @13:06:36.52 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @13:06:51.33 -> Cartridge.unlock @13:06:51.39 Gate.power :heat,:OFF @13:06:51.42 Gate.power :cartridge,:OFF @13:06:51.55 PinPort.in :unlocking @13:06:51.64 PinPort.seek :clear @13:06:52.14 Carousel.in :default @13:06:52.31 Carousel.home.seek 6 @13:06:52.68 Archive.in :unlocking @13:06:52.84 Archive.seek :clear @13:06:52.91 Air.in :unlocking @13:06:53.16 Air.seek :clear @13:06:53.24 Lysis2.in :unlocking @13:06:53.37 Lysis2.seek :clear @13:06:57.95 Lysis1.in :unlocking @13:06:58.12 Lysis1.seek :clear @13:06:58.26 Plunge.in :unlocking @13:06:58.36 Plunge.seek :clear @13:07:16.99 -> Syringe.psi 0.179719822864996 @13:07:28.99 -> exit @13:07:29.01 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @13:08:59.67 Session Begun @13:09:02.85 -> to 6 @13:09:03.17 Carousel.seek 6 @13:09:04.00 Lysis1.in :fast @13:09:04.09 Lysis1.seek :locked @13:09:06.79 Plunge.in :default @13:09:06.88 Plunge.seek :locked @13:09:07.11 Air.in :fast @13:09:07.19 Air.seek :locked @13:09:08.19 Carousel.reconfigure :locked @13:09:08.37 Powering cartridge bus @13:09:11.05 Gate.setIR 1750,5000,0,6 @13:09:11.17 Gate.setIR 0,5000,0,6 @13:09:11.27 PuckHeater.configure :default @13:09:11.36 Gate.power :heat,:ON,limit to 5000mA @13:09:12.06 Mounted lysis Cartridge board#Q05092 @13:09:12.15 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @13:09:12.74 -> Cartridge.sampler.source=nil @13:09:42.09 -> slots {6 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50.0}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:09:55.98 -> slot 6=>[:dry, :lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:13:10.56 -> PhytoSampler.bypassVolume=PhytoSampler.primeVolume=nil @13:13:24.13 -> fire lyseHold_phyto @13:13:24.31 Selecting Cartridge 6 # @13:13:24.77 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:13:25.19 Cmd::Error in detached from preston#2 -- Cannot filter a lysing cartridge @13:13:25.22 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @13:13:26.66 BadNews.email "Cannot filter a lysing cartridge",Subject: "Cmd::Error in detached from preston#2" @13:13:27.50 upsync @13:13:28.84 upsync @13:13:49.86 -> slots {6 => { Type: :lyseHold_phyto, State: :dry}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:14:09.71 -> Cartridge.unlock @13:14:09.77 Gate.power :heat,:OFF @13:14:09.79 Gate.power :cartridge,:OFF @13:14:09.92 Carousel.in :default @13:14:10.06 Carousel.home.seek 6 @13:14:10.55 Air.in :unlocking @13:14:11.17 Air.seek :clear @13:14:12.31 Lysis1.in :unlocking @13:14:12.44 Lysis1.seek :clear @13:14:12.59 Plunge.in :unlocking @13:14:12.67 Plunge.seek :clear @13:14:20.26 -> fire lyseHold_phyto @13:14:20.44 Selecting Cartridge 6 # @13:14:20.82 Carousel.seek 6 @13:14:21.10 Lysis1.in :fast @13:14:21.19 Lysis1.seek :locked @13:14:23.84 Plunge.in :default @13:14:23.97 Plunge.seek :locked @13:14:24.15 Air.in :fast @13:14:24.23 Air.seek :locked @13:14:25.24 Carousel.reconfigure :locked @13:14:25.45 Powering cartridge bus @13:14:28.15 Gate.setIR 1750,5000,0,6 @13:14:28.29 Gate.setIR 0,5000,0,6 @13:14:28.39 PuckHeater.configure :default @13:14:28.49 Gate.power :heat,:ON,limit to 5000mA @13:14:29.18 Mounted lysis Cartridge board#Q05092 @13:14:29.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:14:29.30 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:14:29.69 Preparing to filter cartridge 6 @13:14:30.04 Air.in :default @13:14:30.18 Bypass.open @13:14:30.24 Air.seek :sealed @13:14:31.10 Toroid.in :hold @13:14:31.20 Toroid.seek :crack @13:14:32.10 upsync @13:14:32.91 Toroid.seek :cartridge @13:14:34.07 Toroid.in :default @13:14:34.19 Toroid.seek :clear @13:14:36.04 Exhaust.open @13:14:36.81 Intake.open @13:14:37.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:14:40.23 Vacuum tare is 52% of full scale at 21C (drifted 1%) @13:14:40.49 Pressurizing cartridge to 26psi above 15.6psia @13:14:40.51 SamplePump.setPosition! 0ml @13:14:40.62 Bypass.close @13:14:41.27 Exhaust.close @13:14:42.04 Abs.in :default @13:14:42.18 Abs.hold @13:14:42.44 SamplePump.in :charge @13:14:42.55 SamplePump.seek 2.3ml,42 seconds @13:14:55.03 Abs.stop @13:14:55.14 Intake.close @13:14:55.93 Pressurized cartridge with 0.697ml to 27.3psi @13:14:55.95 Isolated @38.3psia (22.7psi) @13:14:55.98 Verifying cartridge holds at least 0.65..0.85 of 38.3psia @13:14:56.05 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:15:00.72 Cartridge pressure fell 4% to 36.9psia @13:15:00.85 Sampling 50.0ml @13:15:00.88 SamplePump.setPosition! 0ml @13:15:00.97 Exhaust.open @13:15:01.65 Intake.open @13:15:02.52 Abs.in :default @13:15:02.61 Abs.hold @13:15:02.97 SamplePump.in :start @13:15:03.08 SamplePump.seek 50ml,4:40 @13:15:03.66 SamplePump.configure :sample @13:15:06.07 Cartridge 6 filtering 2/50ml at 0.80ml/s with 18.8psi vacuum @13:16:06.14 Cartridge 6 filtering 38/50ml at 0.61ml/s with 23.4psi vacuum @13:16:25.27 Abs.stop @13:16:25.46 Intake.close @13:16:25.51 Exhaust.close @13:16:25.81 Toroid.in :hold @13:16:25.89 Toroid.seek :bypass @13:16:27.56 Toroid.in :default @13:16:27.66 Toroid.seek :clear @13:16:29.31 Sampled 50.0ml @13:16:29.78 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" /home/esp/esp2/utils/auv/cmd.rb:487: warning: Object#type is deprecated; use Object#class @13:16:31.27 Cartridge::Error in PROCESSING -- No Cartridge mounted @13:16:31.34 Thread::ChildDied in detached from preston#2 -- :PROCESSING @13:16:31.42 Waiting to Resume from Checkpoint ":PROCESSING" @13:16:31.53 Gate.power :heat,:OFF @13:16:31.55 Gate.power :cartridge,:OFF @13:16:31.71 Carousel.in :default @13:16:31.85 Carousel.home.seek 6 @13:16:32.12 BadNews.email "No Cartridge mounted",Subject: "Cartridge::Error in PROCESSING" @13:16:32.91 Plunge.seek :locked @13:16:33.21 upsync @13:16:33.57 Air.in :unlocking @13:16:33.77 Air.seek :clear @13:16:33.99 Lysis1.in :unlocking @13:16:34.20 Lysis1.seek :clear @13:16:34.48 BadNews.email ":PROCESSING",Subject: "Thread::ChildDied in detached from preston#2" @13:16:35.32 upsync @13:16:35.88 Gate.power :analytic1,:OFF @13:16:35.90 Gate.power :motor,:OFF @13:16:35.95 Gate.power :core,:OFF @13:16:36.01 Gate.power :main,:OFF @13:16:36.03 Safely stopped and ready to power off @13:16:37.59 upsync @13:27:29.36 -> slots {6 => { Type: :lyseHold_phyto, State: :failedProcessing, filtered: 50.0}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:31:16.93 -> fire lyseHold_phyto @13:31:17.10 Cartridge::None in detached from preston -- No dry lyseHold_phyto cartridges remain # @13:31:17.19 BadNews.email "No dry lyseHold_phyto cartridges remain",Subject: "Cartridge::None in detached from preston" @13:31:20.07 upsync @13:31:26.68 -> slots {6 => { Type: :lyseHold_phyto, State: :failedProcessing, filtered: 50.0}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:31:45.45 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:31:49.01 -> fire lyseHold_phyto # @13:31:49.22 Gate.power :sampler,:OFF,limit to 3500mA @13:31:49.28 Gate.power :skimmer,:OFF,limit to 3500mA @13:31:49.33 Gate.power :main,:ON,limit to 5000mA @13:31:49.37 Gate.power :analytic1,:ON,limit to 4000mA @13:31:49.49 Powering core bus @13:31:52.29 Plunge.configure :default @13:31:52.40 SamplePump.configure :default @13:31:52.52 Toroid.configure :default @13:31:52.64 Carousel.configure :default @13:31:52.75 Abs.configure :default @13:31:52.87 PhaseSeparator.configure :default @13:31:52.98 Archive.configure :default @13:31:53.10 Air.configure :default @13:31:53.18 Lysis1.configure :default @13:31:53.52 Lysis2.configure :default @13:31:53.64 PinPort.configure :default @13:31:53.72 All elf controllers running firmware version 4.92 @13:31:53.75 Gate.power :motor,:ON,limit to 5000mA @13:31:56.74 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:31:56.77 AnalyticModule::Unknown in detached from preston -- SPR @13:31:57.02 Plunge.home.jog 0 @13:31:57.77 PinPort.home.jog 0 @13:31:57.80 Lysis2.home.jog 0 @13:31:57.84 Lysis1.home.jog 0 @13:31:57.89 Archive.home.jog 0 @13:31:57.95 Toroid.home.jog 0 @13:31:58.02 Carousel.home.jog 0 @13:31:58.14 Air.home.jog 0 @13:31:59.02 Plunge.in :homing @13:31:59.30 Plunge.jog 1608 @13:31:59.60 PinPort.in :homing @13:31:59.65 Lysis2.in :homing @13:31:59.70 Air.in :homing @13:31:59.75 Toroid.in :homing @13:31:59.78 Lysis1.in :homing @13:31:59.89 Archive.in :homing @13:32:00.17 PinPort.jog -105 @13:32:00.24 Lysis2.jog 1401 @13:32:00.36 Air.jog 1371 @13:32:00.46 Lysis1.jog 1393 @13:32:00.47 Archive.jog 1404 @13:32:00.56 Toroid.jog 632 @13:32:01.03 PinPort.setRawPosition! 0 @13:32:01.05 Plunge.setRawPosition! 50 @13:32:01.21 PinPort.seek :home @13:32:01.25 Plunge.seek :home @13:32:01.48 PinPort.in :default @13:32:01.65 Plunge.in :default @13:32:01.86 Air.setRawPosition! 50 @13:32:01.90 Lysis2.setRawPosition! 50 @13:32:02.02 Air.seek :home @13:32:02.15 Lysis2.seek :home @13:32:02.36 Lysis1.setRawPosition! 50 @13:32:02.40 Archive.setRawPosition! 50 @13:32:02.53 Lysis1.seek :home @13:32:02.65 Archive.seek :home @13:32:02.83 Air.in :default @13:32:02.86 Lysis2.in :default @13:32:03.10 Archive.in :default @13:32:03.13 Toroid.setRawPosition! 0 @13:32:03.17 Lysis1.in :default @13:32:03.38 Toroid.seek :home @13:32:03.96 Toroid.in :default @13:32:04.23 Toroid.seek :clear @13:32:05.93 Carousel.in :default @13:32:06.10 Carousel.home.seek 6 @13:32:06.42 Selecting Cartridge 6 @13:32:06.74 Carousel.seek 6 @13:32:07.01 Lysis1.in :fast @13:32:07.09 Lysis1.seek :locked @13:32:09.76 Plunge.seek :locked @13:32:09.94 Air.in :fast @13:32:10.03 Air.seek :locked @13:32:11.04 Carousel.reconfigure :locked @13:32:11.23 Powering cartridge bus @13:32:13.90 Gate.setIR 1750,5000,0,6 @13:32:14.04 Gate.setIR 0,5000,0,6 @13:32:14.16 PuckHeater.configure :default @13:32:14.25 Gate.power :heat,:ON,limit to 5000mA @13:32:14.95 Mounted lysis Cartridge board#Q05092 @13:32:15.00 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:32:15.15 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:32:15.47 Preparing to filter cartridge 6 @13:32:15.81 Air.in :default @13:32:15.95 Bypass.open @13:32:16.03 Air.seek :sealed @13:32:16.88 Toroid.in :hold @13:32:17.09 Toroid.seek :crack @13:32:17.89 upsync @13:32:18.74 Toroid.seek :cartridge @13:32:19.90 Toroid.in :default @13:32:20.00 Toroid.seek :clear @13:32:21.82 Exhaust.open @13:32:22.57 Intake.open @13:32:23.32 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:32:25.94 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:32:26.25 Pressurizing cartridge to 26psi above 15.6psia @13:32:26.27 SamplePump.setPosition! 0ml @13:32:26.38 Bypass.close @13:32:27.05 Exhaust.close @13:32:27.83 Abs.in :default @13:32:27.92 Abs.hold @13:32:28.21 SamplePump.in :charge @13:32:28.32 SamplePump.seek 2.3ml,42 seconds @13:32:39.17 Abs.stop @13:32:39.26 Intake.close @13:32:40.01 Pressurized cartridge with 0.525ml to 26.5psi @13:32:40.03 Isolated @38.0psia (22.4psi) @13:32:40.08 Verifying cartridge holds at least 0.65..0.85 of 38.0psia @13:32:40.19 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:32:44.80 Cartridge pressure fell 1% to 37.8psia @13:32:44.93 Sampling 50.0ml @13:32:44.95 SamplePump.setPosition! 0ml @13:32:45.06 Exhaust.open @13:32:45.73 Intake.open @13:32:46.57 Abs.in :default @13:32:46.67 Abs.hold @13:32:47.02 SamplePump.in :start @13:32:47.13 SamplePump.seek 50ml,4:40 @13:32:47.71 SamplePump.configure :sample @13:32:50.15 Cartridge 6 filtering 2/50ml at 0.78ml/s with 18.9psi vacuum @13:33:50.21 Cartridge 6 filtering 38/50ml at 0.61ml/s with 20.5psi vacuum @13:34:09.23 Abs.stop @13:34:09.41 Intake.close @13:34:09.48 Exhaust.close @13:34:09.76 Toroid.in :hold @13:34:09.85 Toroid.seek :bypass @13:34:11.51 Toroid.in :default @13:34:11.61 Toroid.seek :clear @13:34:13.84 Sampled 50.0ml @13:34:14.34 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @13:34:14.80 Handoff.select :waste @13:34:15.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:34:17.66 upsync @13:34:21.63 Syringe tare is 52% of full scale at 21C (drifted 1%) @13:34:21.67 PinPort.in :locking @13:34:21.76 PinPort.seek :locked @13:34:22.27 Archive.in :fast @13:34:22.33 Lysis2.in :fast @13:34:22.48 Lysis1.seek 1350uL @13:34:22.62 Archive.seek :full @13:34:22.68 Lysis2.seek :empty @13:34:30.81 Plunge.seek :reagent @13:34:31.75 Evacuating puck with filter bubble point of 24psi @13:34:31.79 PinPort.seek :locked @13:34:31.92 Handoff.select :waste @13:34:31.97 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:34:39.58 Air syringe pumping up to 1 strokes to reach 4psi @13:34:39.59 Air stroke #1 of 1 @13:34:39.75 Air.in :pressurize @13:34:39.84 Air.seek :pressurized @13:34:45.49 0.9psi after pressurize @13:34:45.61 Air.in :compress @13:34:45.72 Air.seek :evac @13:34:48.04 Reached 3.7 of 4.0psi after pumping 1551uL (0.97 strokes) @13:34:48.06 Air.in :default @13:34:48.20 Air.hold @13:34:48.39 Handoff.select :blocked @13:34:48.56 Overpressure in PROCESSING -- Waste port clogged (1.7psi) @13:34:48.59 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 179uL (sensed 175uL)" @13:34:48.63 BadNews.email "Waste port clogged (1.7psi)",Subject: "Overpressure in PROCESSING" @13:34:52.03 upsync @13:35:51.93 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:37:26.90 -> fire lyseHold_phyto @13:37:27.10 Selecting Cartridge 6 # @13:37:27.56 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:37:28.06 Cmd::Error in detached from preston#3 -- Cannot filter a lysing cartridge @13:37:28.10 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @13:37:28.98 BadNews.email "Cannot filter a lysing cartridge",Subject: "Cmd::Error in detached from preston#3" @13:37:29.77 upsync @13:37:31.26 upsync @13:38:13.18 -> slots {6 => { Type: :lyseHold_phyto, State: :dry}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:38:16.51 -> Cartridge.unlock @13:38:16.56 Gate.power :heat,:OFF @13:38:16.59 Gate.power :cartridge,:OFF @13:38:16.71 PinPort.in :unlocking @13:38:16.79 PinPort.seek :clear @13:38:17.25 Carousel.in :default @13:38:17.40 Carousel.home.seek 6 @13:38:17.79 Archive.in :unlocking @13:38:17.98 Archive.seek :clear @13:38:18.05 Air.in :unlocking @13:38:18.84 Air.seek :clear @13:38:18.97 Lysis2.in :unlocking @13:38:19.06 Lysis2.seek :clear @13:38:23.64 Lysis1.in :unlocking @13:38:23.79 Lysis1.seek :clear @13:38:23.92 Plunge.in :unlocking @13:38:24.01 Plunge.seek :clear @13:38:29.71 -> fire lyseHold_phyto @13:38:29.92 Selecting Cartridge 6 # @13:38:30.33 Carousel.seek 6 @13:38:30.60 Lysis1.in :fast @13:38:30.70 Lysis1.seek :locked @13:38:33.35 Plunge.in :default @13:38:33.47 Plunge.seek :locked @13:38:33.64 Air.in :fast @13:38:33.73 Air.seek :locked @13:38:34.74 Carousel.reconfigure :locked @13:38:34.94 Powering cartridge bus @13:38:37.60 Gate.setIR 1750,5000,0,6 @13:38:37.73 Gate.setIR 0,5000,0,6 @13:38:37.83 PuckHeater.configure :default @13:38:37.92 Gate.power :heat,:ON,limit to 5000mA @13:38:38.63 Mounted lysis Cartridge board#Q05092 @13:38:38.69 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:38:38.76 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:38:39.24 Preparing to filter cartridge 6 @13:38:39.66 Air.in :default @13:38:39.80 Bypass.open @13:38:39.85 Air.seek :sealed @13:38:40.82 Toroid.in :hold @13:38:41.00 Toroid.seek :crack @13:38:41.50 upsync @13:38:42.66 Toroid.seek :cartridge @13:38:44.37 Toroid.in :default @13:38:44.55 Toroid.seek :clear @13:38:46.40 Exhaust.open @13:38:47.13 Intake.open @13:38:47.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:38:50.50 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:38:50.68 Pressurizing cartridge to 26psi above 15.7psia @13:38:50.71 SamplePump.setPosition! 0ml @13:38:50.81 Bypass.close @13:38:51.44 Exhaust.close @13:38:52.24 Abs.in :default @13:38:52.33 Abs.hold @13:38:52.59 SamplePump.in :charge @13:38:52.69 SamplePump.seek 2.3ml,42 seconds @13:38:55.00 Abs.stop @13:38:55.10 Intake.close @13:38:55.85 Pressurized cartridge with 0.021ml to 26.3psi @13:38:55.87 Isolated @34.2psia (18.5psi) @13:38:55.91 Verifying cartridge holds at least 0.65..0.85 of 34.2psia @13:38:55.99 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:39:01.60 Cartridge pressure fell 28% to 24.5psia @13:39:01.77 Toroid.in :hold @13:39:01.87 Toroid.seek :bypass @13:39:03.55 Toroid.in :default @13:39:03.65 Toroid.seek :clear @13:39:05.40 Cartridge::Sampler::Leaky in FILTERING -- Retained only 24.5psia @13:39:05.43 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @13:39:05.47 BadNews.email "Retained only 24.5psia",Subject: "Cartridge::Sampler::Leaky in FILTERING" @13:39:07.81 upsync @13:42:50.97 -> Thread[:filtering].resume @13:42:51.14 NoMethodError in preston -- private method `resume' called for nil:NilClass @13:43:00.01 -> Thread[:Filtering].resume @13:43:00.20 NoMethodError in preston -- private method `resume' called for nil:NilClass @13:43:09.57 -> Thread[:filtering].recover @13:43:09.73 NoMethodError in preston -- private method `recover' called for nil:NilClass @13:43:17.65 -> fire lyseHold_phyto @13:43:17.83 Cartridge::None in detached from preston#4 -- No dry lyseHold_phyto cartridges remain # @13:43:17.91 BadNews.email "No dry lyseHold_phyto cartridges remain",Subject: "Cartridge::None in detached from preston#4" @13:43:20.03 upsync @13:43:29.04 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:43:31.21 -> fire lyseHold_phyto @13:43:31.41 Selecting Cartridge 6 # @13:43:31.90 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:43:32.43 Cmd::Error in detached from preston#4 -- Cannot filter a leaky cartridge @13:43:32.47 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @13:43:33.41 BadNews.email "Cannot filter a leaky cartridge",Subject: "Cmd::Error in detached from preston#4" @13:43:34.19 upsync @13:43:35.64 upsync @13:44:04.94 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:44:16.59 -> Cartridge.activate @13:44:16.82 Gate.setIR 1750,5000,0,6 @13:44:16.95 Gate.setIR 0,5000,0,6 @13:44:17.05 PuckHeater.configure :default @13:44:17.14 Gate.power :heat,:ON,limit to 5000mA @13:44:19.82 Mounted lysis Cartridge board#Q05092 @13:44:19.89 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @13:44:23.22 -> fire lyseHold_phyto @13:44:23.41 Selecting Cartridge 6 # @13:44:23.92 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @13:44:25.05 Preparing to filter cartridge 6 @13:44:25.79 Air.seek :sealed @13:44:26.05 Bypass.open @13:44:26.33 Cmd::Error in detached from preston#3 -- Cannot process while in PRIMING state @13:44:26.35 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @13:44:26.68 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @13:44:27.27 Toroid.in :hold @13:44:27.60 Toroid.seek :crack @13:44:27.80 upsync @13:44:28.74 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from preston#3" @13:44:29.55 upsync @13:44:29.63 Toroid.seek :cartridge @13:44:30.94 Toroid.in :default @13:44:31.13 Toroid.seek :clear @13:44:31.59 upsync @13:44:33.01 Exhaust.open @13:44:33.93 Pressurizing cartridge to 26psi above 15.5psia @13:44:33.96 SamplePump.setPosition! 0ml @13:44:34.05 Intake.open @13:44:34.80 Bypass.close @13:44:35.49 Exhaust.close @13:44:36.33 Abs.in :default @13:44:36.41 Abs.hold @13:44:36.69 SamplePump.seek 2.3ml,42 seconds @13:44:38.78 Abs.stop @13:44:39.45 Intake.close @13:44:40.21 Pressurized cartridge with 0.018ml to 26.5psi @13:44:40.23 Isolated @29.2psia (13.7psi) @13:44:40.27 Verifying cartridge holds at least 0.65..0.85 of 29.2psia @13:44:40.34 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:44:44.94 Cartridge pressure fell 5% to 27.9psia @13:44:45.08 Sampling 50.0ml @13:44:45.10 SamplePump.setPosition! 0ml @13:44:45.20 Exhaust.open @13:44:45.91 Intake.open @13:44:46.80 Abs.in :default @13:44:46.89 Abs.hold @13:44:47.05 Sea 7.7m deep (26.0psia) @13:44:47.29 SamplePump.in :start @13:44:47.40 SamplePump.seek 50ml,4:40 @13:44:47.98 SamplePump.configure :sample @13:44:50.40 Cartridge 6 filtering 0/50ml at 0.00ml/s with 26.8psi vacuum @13:44:50.53 Flow estimate reduced to 0.65ml/s @13:44:50.61 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 12.0psi @13:44:58.71 Intake.close @13:44:58.85 Exhaust.close @13:44:59.11 Toroid.in :hold @13:44:59.21 Toroid.seek :bypass @13:45:00.90 Toroid.in :default @13:45:00.99 Toroid.seek :clear @13:45:02.65 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:45:02.69 Abs.stop @13:45:02.97 Sampled 0.0ml @13:45:03.41 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" /home/esp/esp2/utils/auv/cmd.rb:487: warning: Object#type is deprecated; use Object#class @13:45:04.35 Cartridge::Error in PROCESSING -- No Cartridge mounted @13:45:04.44 Thread::ChildDied in detached from preston#5 -- :PROCESSING @13:45:04.47 Waiting to Resume from Checkpoint ":PROCESSING" @13:45:04.55 Gate.power :heat,:OFF @13:45:04.60 Gate.power :cartridge,:OFF @13:45:04.81 Carousel.in :default @13:45:04.97 Carousel.home.seek 6 @13:45:05.40 BadNews.email "No Cartridge mounted",Subject: "Cartridge::Error in PROCESSING" @13:45:05.87 Plunge.seek :locked @13:45:06.42 upsync @13:45:06.71 Air.in :unlocking @13:45:07.44 Air.seek :clear @13:45:07.74 Lysis1.in :unlocking @13:45:07.93 Lysis1.seek :clear @13:45:08.31 BadNews.email ":PROCESSING",Subject: "Thread::ChildDied in detached from preston#5" @13:45:09.15 upsync @13:45:09.57 Gate.power :analytic1,:OFF @13:45:09.59 Gate.power :motor,:OFF @13:45:09.64 Gate.power :core,:OFF @13:45:09.68 Gate.power :main,:OFF @13:45:09.70 Safely stopped and ready to power off @13:47:24.55 -> fire lyseHold_phyto @13:47:24.75 Cartridge::None in detached from preston -- No dry lyseHold_phyto cartridges remain # @13:47:30.11 -> slots {6 => { Type: :lyseHold_phyto, State: :failedProcessing}, [34,15] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, [54,28] => { Type: :lyseHold_phyto, State: :leaky}, [41,40] => { Type: :archive_bac, State: :preserving}, 43 => { Type: :lyseHold_phyto, State: :analyzing, filtered: 50, lysed: 0}, 48 => { Type: :lyseHold_phyto, State: :missing}} @13:47:39.00 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @13:47:42.66 -> fire lyseHold_phyto # @13:47:42.89 Gate.power :sampler,:OFF,limit to 3500mA @13:47:42.94 Gate.power :skimmer,:OFF,limit to 3500mA @13:47:43.00 Gate.power :main,:ON,limit to 5000mA @13:47:43.04 Gate.power :analytic1,:ON,limit to 4000mA @13:47:43.16 Powering core bus @13:47:45.94 Plunge.configure :default @13:47:46.07 SamplePump.configure :default @13:47:46.22 Toroid.configure :default @13:47:46.33 Carousel.configure :default @13:47:46.43 Abs.configure :default @13:47:46.55 PhaseSeparator.configure :default @13:47:46.65 Archive.configure :default @13:47:46.77 Air.configure :default @13:47:46.85 Lysis1.configure :default @13:47:47.20 Lysis2.configure :default @13:47:47.30 PinPort.configure :default @13:47:47.38 All elf controllers running firmware version 4.92 @13:47:47.41 Gate.power :motor,:ON,limit to 5000mA @13:47:50.41 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:47:50.44 AnalyticModule::Unknown in detached from preston -- SPR @13:47:50.97 Plunge.home.jog 0 @13:47:51.20 Toroid.home.jog 0 @13:47:51.24 Carousel.home.jog 0 @13:47:51.83 Lysis2.home.jog 0 @13:47:51.89 Air.home.jog 0 @13:47:52.00 Lysis1.home.jog 0 @13:47:52.05 Archive.home.jog 0 @13:47:52.15 PinPort.home.jog 0 @13:47:52.82 Toroid.in :homing @13:47:52.98 Plunge.in :homing @13:47:53.70 Plunge.jog 1611 @13:47:53.73 Toroid.jog 625 @13:47:54.05 PinPort.in :homing @13:47:54.12 Lysis2.in :homing @13:47:54.19 Air.in :homing @13:47:54.23 Archive.in :homing @13:47:54.29 Lysis1.in :homing @13:47:54.53 PinPort.jog -106 @13:47:54.60 Lysis2.jog 1406 @13:47:54.79 Lysis1.jog 1394 @13:47:54.85 Air.jog 1374 @13:47:54.89 Archive.jog 1404 @13:47:55.29 PinPort.setRawPosition! 0 @13:47:55.31 Plunge.setRawPosition! 50 @13:47:55.46 PinPort.seek :home @13:47:55.50 Plunge.seek :home @13:47:55.77 PinPort.in :default @13:47:55.90 Plunge.in :default @13:47:56.02 Toroid.setRawPosition! 0 @13:47:56.17 Toroid.seek :home @13:47:56.35 Lysis1.setRawPosition! 50 @13:47:56.41 Air.setRawPosition! 50 @13:47:56.45 Archive.setRawPosition! 50 @13:47:56.52 Lysis2.setRawPosition! 50 @13:47:56.55 Toroid.in :default @13:47:56.84 Air.seek :home @13:47:56.90 Lysis1.seek :home @13:47:56.94 Archive.seek :home @13:47:57.01 Lysis2.seek :home @13:47:57.44 Air.in :default @13:47:57.46 Lysis1.in :default @13:47:57.70 Archive.in :default @13:47:57.73 Lysis2.in :default @13:47:58.16 Toroid.seek :clear @13:47:59.86 Carousel.in :default @13:48:00.02 Carousel.home.seek 6 @13:48:00.29 Selecting Cartridge 6 @13:48:00.62 Carousel.seek 6 @13:48:00.90 Lysis1.in :fast @13:48:01.00 Lysis1.seek :locked @13:48:03.67 Plunge.seek :locked @13:48:03.87 Air.in :fast @13:48:03.95 Air.seek :locked @13:48:04.95 Carousel.reconfigure :locked @13:48:05.13 Powering cartridge bus @13:48:07.79 Gate.setIR 1750,5000,0,6 @13:48:07.92 Gate.setIR 0,5000,0,6 @13:48:08.02 PuckHeater.configure :default @13:48:08.15 Gate.power :heat,:ON,limit to 5000mA @13:48:09.29 Mounted lysis Cartridge board#Q05092 @13:48:09.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:48:09.79 Preparing to filter cartridge 6 @13:48:10.07 Air.in :default @13:48:10.23 Bypass.open @13:48:10.26 Air.seek :sealed @13:48:11.08 Toroid.in :hold @13:48:11.16 Toroid.seek :crack @13:48:12.74 Toroid.seek :cartridge @13:48:13.89 Toroid.in :default @13:48:13.99 Toroid.seek :clear @13:48:15.83 Exhaust.open @13:48:16.54 Intake.open @13:48:17.34 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:48:19.96 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:48:20.18 Pressurizing cartridge to 26psi above 15.6psia @13:48:20.20 SamplePump.setPosition! 0ml @13:48:20.29 Bypass.close @13:48:20.93 Exhaust.close @13:48:21.69 Abs.in :default @13:48:21.78 Abs.hold @13:48:22.04 SamplePump.in :charge @13:48:22.19 SamplePump.seek 2.3ml,42 seconds @13:48:24.52 Abs.stop @13:48:24.63 Intake.close @13:48:25.39 Pressurized cartridge with 0.021ml to 26.1psi @13:48:25.41 Isolated @33.4psia (17.8psi) @13:48:25.45 Verifying cartridge holds at least 0.65..0.85 of 33.4psia @13:48:25.51 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:48:31.13 Cartridge pressure fell 26% to 24.7psia @13:48:31.29 Toroid.in :hold @13:48:31.39 Toroid.seek :bypass @13:48:33.68 Toroid.in :default @13:48:33.79 Toroid.seek :clear @13:48:35.53 Cartridge::Sampler::Leaky in FILTERING -- Retained only 24.7psia @13:48:35.56 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @13:49:19.98 -> fire lyseHold_phyto @13:49:20.20 Cartridge::None in detached from preston#6 -- No dry lyseHold_phyto cartridges remain # @13:49:33.70 -> Cartridge.unlock @13:49:33.77 Gate.power :heat,:OFF @13:49:33.78 Gate.power :cartridge,:OFF @13:49:33.91 Carousel.in :default @13:49:34.07 Carousel.home.seek 6 @13:49:34.55 Air.in :unlocking @13:49:34.70 Air.seek :clear @13:49:36.17 Lysis1.in :unlocking @13:49:36.34 Lysis1.seek :clear @13:49:36.45 Plunge.in :unlocking @13:49:36.56 Plunge.seek :clear /suscript/connectNetwork: No such file or directory @13:50:08.53 Email::SendTimeout in Qmail -- no response after 5:00 @13:50:08.57 Retry #1 of 10 after 10:00 @14:00:10.98 BadNews.email "No dry lyseHold_phyto cartridges remain",Subject: "Cartridge::None in detached from preston" @14:00:11.95 upsync @14:00:12.59 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @14:00:13.39 upsync @14:00:15.03 BadNews.email "Retained only 24.7psia",Subject: "Cartridge::Sampler::Leaky in FILTERING" @14:00:15.90 upsync @14:00:17.37 BadNews.email "No dry lyseHold_phyto cartridges remain",Subject: "Cartridge::None in detached from preston#6" @14:00:18.23 upsync @14:00:20.29 upsync @14:08:14.40 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @14:08:17.75 -> fire lyseHold_phyto @14:08:17.97 Selecting Cartridge 6 # @14:08:18.40 Carousel.seek 6 @14:08:18.69 Lysis1.in :fast @14:08:18.79 Lysis1.seek :locked @14:08:21.45 Plunge.in :default @14:08:21.56 Plunge.seek :locked @14:08:21.76 Air.in :fast @14:08:21.85 Air.seek :locked @14:08:22.85 Carousel.reconfigure :locked @14:08:23.05 Powering cartridge bus @14:08:25.72 Gate.setIR 1750,5000,0,6 @14:08:25.86 Gate.setIR 0,5000,0,6 @14:08:25.95 PuckHeater.configure :default @14:08:26.05 Gate.power :heat,:ON,limit to 5000mA @14:08:26.76 Mounted lysis Cartridge board#Q05092 @14:08:26.82 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:08:26.87 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @14:08:27.33 Preparing to filter cartridge 6 @14:08:28.08 Air.in :default @14:08:28.23 Bypass.open @14:08:28.37 Air.seek :sealed @14:08:29.13 Cmd::Error in detached from preston -- Cannot process while in PRIMING state @14:08:29.30 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @14:08:29.43 Toroid.in :hold @14:08:29.52 Toroid.seek :crack @14:08:29.81 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @14:08:30.77 upsync @14:08:31.24 Toroid.seek :cartridge @14:08:31.46 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from preston" @14:08:32.24 upsync @14:08:32.63 Toroid.in :default @14:08:32.76 Toroid.seek :clear @14:08:34.14 upsync @14:08:34.66 Exhaust.open @14:08:35.59 Pressurizing cartridge to 26psi above 15.4psia @14:08:35.61 SamplePump.setPosition! 0ml @14:08:35.73 Intake.open @14:08:36.42 Bypass.close @14:08:37.11 Exhaust.close @14:08:37.93 Abs.in :default @14:08:38.01 Abs.hold @14:08:38.32 SamplePump.seek 2.3ml,42 seconds @14:08:40.96 Abs.stop @14:08:41.07 Intake.close @14:08:41.80 Pressurized cartridge with 0.029ml to 26.3psi @14:08:41.82 Isolated @32.3psia (16.9psi) @14:08:41.84 Verifying cartridge holds at least 0.65..0.85 of 32.3psia @14:08:41.92 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:08:46.71 Cartridge pressure fell 12% to 28.6psia @14:08:46.85 Sampling 50.0ml @14:08:46.87 SamplePump.setPosition! 0ml @14:08:46.97 Exhaust.open @14:08:47.68 Intake.open @14:08:48.55 Abs.in :default @14:08:48.64 Abs.hold @14:08:48.83 Sea 7.6m deep (26.0psia) @14:08:49.07 SamplePump.in :start @14:08:49.17 SamplePump.seek 50ml,4:40 @14:08:49.76 SamplePump.configure :sample @14:08:52.20 Cartridge 6 filtering 0/50ml at 0.00ml/s with 25.4psi vacuum @14:09:07.92 -> Cmd.stop @14:09:07.96 Abs.stop @14:09:08.19 Intake.close @14:09:08.27 Exhaust.close @14:09:08.56 Toroid.in :hold @14:09:08.62 Toroid.seek :bypass @14:09:10.27 Toroid.in :default @14:09:10.36 Toroid.seek :clear @14:09:12.19 Toroid.stop @14:09:12.66 Gate.power :heat,:OFF @14:09:12.69 Gate.power :cartridge,:OFF @14:09:12.86 GoodNews.email "After filtering 0.1ml",Subject: "Processing cartridge in slot 6" @14:09:12.90 Carousel.in :default @14:09:13.00 Cartridge::Error in detached from preston#6 -- Not Mounted @14:09:13.10 Waiting to Resume from Checkpoint "Selecting Cartridge 6" @14:09:13.38 Carousel.home.seek 6 @14:09:13.92 Plunge.seek :locked @14:09:14.46 Air.in :unlocking @14:09:14.64 Air.seek :clear @14:09:14.83 Lysis1.in :unlocking @14:09:15.14 BadNews.email "Not Mounted",Subject: "Cartridge::Error in detached from preston#6" @14:09:15.31 Lysis1.seek :clear @14:09:16.48 upsync @14:09:17.07 Gate.power :analytic1,:OFF @14:09:17.14 Gate.power :motor,:OFF @14:09:17.24 Gate.power :core,:OFF @14:09:17.29 Gate.power :main,:OFF @14:09:17.37 Safely stopped and ready to power off :STOPPED @14:09:19.97 upsync @14:09:33.99 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @14:09:36.24 -> fire lyseHold_phyto # @14:09:36.50 Gate.power :sampler,:OFF,limit to 3500mA @14:09:36.57 Gate.power :skimmer,:OFF,limit to 3500mA @14:09:36.62 Gate.power :main,:ON,limit to 5000mA @14:09:36.68 Gate.power :analytic1,:ON,limit to 4000mA @14:09:36.78 Powering core bus @14:09:39.59 Plunge.configure :default @14:09:39.70 SamplePump.configure :default @14:09:39.81 Toroid.configure :default @14:09:40.03 Carousel.configure :default @14:09:40.17 Abs.configure :default @14:09:40.30 PhaseSeparator.configure :default @14:09:40.41 Archive.configure :default @14:09:40.79 Air.configure :default @14:09:40.89 Lysis1.configure :default @14:09:40.97 Lysis2.configure :default @14:09:41.08 PinPort.configure :default @14:09:41.18 All elf controllers running firmware version 4.92 @14:09:41.21 Gate.power :motor,:ON,limit to 5000mA @14:09:44.04 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @14:09:44.07 AnalyticModule::Unknown in detached from preston#7 -- SPR @14:09:44.54 Toroid.home.jog 0 @14:09:44.73 Plunge.home.jog 0 @14:09:45.34 Lysis2.home.jog 0 @14:09:45.39 Air.home.jog 0 @14:09:45.43 Carousel.home.jog 0 @14:09:45.49 PinPort.home.jog 0 @14:09:45.54 Archive.home.jog 0 @14:09:45.60 Lysis1.home.jog 0 @14:09:46.48 Plunge.in :homing @14:09:46.56 Toroid.in :homing @14:09:46.73 Plunge.jog 1608 @14:09:46.78 Toroid.jog 623 @14:09:47.09 Lysis2.in :homing @14:09:47.14 Lysis1.in :homing @14:09:47.17 Archive.in :homing @14:09:47.22 PinPort.in :homing @14:09:47.27 Air.in :homing @14:09:47.49 Lysis2.jog 1405 @14:09:47.70 PinPort.jog -104 @14:09:47.74 Lysis1.jog 1389 @14:09:47.82 Archive.jog 1397 @14:09:47.87 Air.jog 1374 @14:09:48.85 PinPort.setRawPosition! 0 @14:09:48.89 Lysis2.setRawPosition! 50 @14:09:48.96 Plunge.setRawPosition! 50 @14:09:49.13 Lysis2.seek :home @14:09:49.20 PinPort.seek :home @14:09:49.25 Plunge.seek :home @14:09:49.64 Lysis2.in :default @14:09:49.69 PinPort.in :default @14:09:49.76 Toroid.setRawPosition! 0 @14:09:49.95 Toroid.seek :home @14:09:50.17 Lysis1.setRawPosition! 50 @14:09:50.23 Air.setRawPosition! 50 @14:09:50.28 Plunge.in :default @14:09:50.33 Archive.setRawPosition! 50 @14:09:50.38 Toroid.in :default @14:09:50.69 Air.seek :home @14:09:50.74 Lysis1.seek :home @14:09:50.78 Archive.seek :home @14:09:51.14 Air.in :default @14:09:51.28 Archive.in :default @14:09:51.31 Lysis1.in :default @14:09:51.74 Toroid.seek :clear @14:09:53.41 Carousel.in :default @14:09:53.58 Carousel.home.seek 6 @14:09:53.88 Selecting Cartridge 6 @14:09:54.25 Carousel.seek 6 @14:09:54.52 Lysis1.in :fast @14:09:54.64 Lysis1.seek :locked @14:09:57.30 Plunge.seek :locked @14:09:57.52 Air.in :fast @14:09:57.59 Air.seek :locked @14:09:58.60 Carousel.reconfigure :locked @14:09:58.78 Powering cartridge bus @14:10:01.44 Gate.setIR 1750,5000,0,6 @14:10:01.57 Gate.setIR 0,5000,0,6 @14:10:01.67 PuckHeater.configure :default @14:10:01.77 Gate.power :heat,:ON,limit to 5000mA @14:10:02.48 Mounted lysis Cartridge board#Q05092 @14:10:02.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:10:02.60 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @14:10:03.10 Preparing to filter cartridge 6 @14:10:04.07 Air.in :default @14:10:04.26 Bypass.open @14:10:04.33 Air.seek :sealed @14:10:05.47 Toroid.in :hold @14:10:05.66 Toroid.seek :crack @14:10:06.25 upsync @14:10:07.35 Toroid.seek :cartridge @14:10:08.51 Toroid.in :default @14:10:08.61 Toroid.seek :clear @14:10:10.44 Exhaust.open @14:10:11.17 Intake.open @14:10:11.92 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:10:14.55 Vacuum tare is 53% of full scale at 21C (drifted 1%) @14:10:14.74 Pressurizing cartridge to 26psi above 15.5psia @14:10:14.76 SamplePump.setPosition! 0ml @14:10:14.86 Bypass.close @14:10:15.55 Exhaust.close @14:10:16.31 Abs.in :default @14:10:16.40 Abs.hold @14:10:16.66 SamplePump.in :charge @14:10:16.77 SamplePump.seek 2.3ml,42 seconds @14:10:29.54 Abs.stop @14:10:29.64 Intake.close @14:10:30.39 Pressurized cartridge with 0.730ml to 27.0psi @14:10:30.41 Isolated @37.9psia (22.3psi) @14:10:30.45 Verifying cartridge holds at least 0.65..0.85 of 37.9psia @14:10:30.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:10:35.13 Cartridge pressure fell 3% to 36.7psia @14:10:35.26 Sampling 50.0ml @14:10:35.29 SamplePump.setPosition! 0ml @14:10:35.41 Exhaust.open @14:10:36.02 Intake.open @14:10:37.45 Abs.in :default @14:10:37.63 Abs.hold @14:10:38.00 SamplePump.in :start @14:10:38.13 SamplePump.seek 50ml,4:40 @14:10:38.69 SamplePump.configure :sample @14:10:41.09 Cartridge 6 filtering 1/50ml at 0.67ml/s with 22.7psi vacuum @14:11:41.15 Cartridge 6 filtering 29/50ml at 0.46ml/s with 11.3psi vacuum 5.6m deep (22.9psia) @14:12:26.23 Abs.stop @14:12:26.41 Intake.close @14:12:26.50 Exhaust.close @14:12:26.75 Toroid.in :hold @14:12:26.85 Toroid.seek :bypass @14:12:28.52 Toroid.in :default @14:12:28.63 Toroid.seek :clear @14:12:30.29 Sampled 50.0ml @14:12:30.75 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @14:12:31.27 Handoff.select :waste @14:12:31.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:12:33.26 upsync @14:12:38.14 Syringe tare is 52% of full scale at 21C (drifted 1%) @14:12:38.18 PinPort.in :locking @14:12:38.31 PinPort.seek :locked @14:12:38.82 Archive.in :fast @14:12:38.87 Lysis2.in :fast @14:12:38.98 Lysis1.seek 1350uL @14:12:39.14 Archive.seek :full @14:12:39.21 Lysis2.seek :empty @14:12:47.90 Plunge.seek :reagent @14:12:48.85 Evacuating puck with filter bubble point of 24psi @14:12:48.88 PinPort.seek :locked @14:12:49.02 Handoff.select :waste @14:12:49.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:12:55.69 Air syringe pumping up to 1 strokes to reach 4psi @14:12:55.71 Air stroke #1 of 1 @14:12:55.85 Air.in :pressurize @14:12:55.93 Air.seek :pressurized @14:13:01.58 1.1psi after pressurize @14:13:01.61 Air.in :compress @14:13:01.72 Air.seek :evac @14:13:04.77 1.5psi after compress @14:13:15.37 0.1psi after compress (stable) @14:13:15.39 Air.in :default @14:13:15.49 Air.hold @14:13:15.74 at 0.0psi Opened to waste @14:13:15.76 Handoff.select :blocked @14:13:15.94 Archive syringe pumping up to 8 strokes to reach 24psi @14:13:15.96 Archive stroke #1 of 8 @14:13:16.13 Archive.in :pressurize @14:13:16.22 Archive.seek :pressurized @14:13:28.70 1.0psi after pressurize @14:13:34.30 0.4psi after pressurize (stable) @14:13:34.42 Archive.in :compress @14:13:34.54 Archive.seek :empty @14:13:50.30 8.1psi after compress @14:14:02.89 8.5psi after compress (stable) @14:14:02.92 Archive.in :gulp @14:14:03.05 Archive.seek :topVent @14:14:09.40 9.9psi after gulp @14:14:14.99 9.4psi after gulp (stable) @14:14:15.00 Archive stroke #2 of 8 @14:14:15.15 Archive.in :pressurize @14:14:15.23 Archive.seek :pressurized @14:14:27.70 10.0psi after pressurize @14:14:33.29 9.3psi after pressurize (stable) @14:14:33.32 Archive.in :compress @14:14:33.44 Archive.seek :empty @14:14:49.69 21.5psi after compress @14:14:51.91 Reached 24.1 of 24.0psi after pumping 3460uL (2.00 strokes) @14:14:51.94 Archive.in :default @14:14:52.05 Archive.hold @14:14:52.21 Handoff.select :waste @14:14:52.37 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:15:04.47 delay 1:00 @14:16:04.50 Handoff.select :blocked @14:16:04.67 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:16:27.27 Handoff.select :waste @14:16:27.50 1.7psi at evacEnd @14:16:27.52 Handoff.select :blocked @14:16:27.69 Archive.in :fast @14:16:27.78 Archive.seek :topVent @14:16:35.00 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:16:41.60 Stable @1.3psi after Handoff blocked @14:16:41.63 Archive syringe pumping up to 8 strokes to reach 25psi @14:16:41.66 Archive stroke #1 of 8 @14:16:41.80 Archive.in :pressurize @14:16:41.89 Archive.seek :pressurized @14:16:54.37 2.4psi after pressurize @14:16:54.41 Archive.in :compress @14:16:54.52 Archive.seek :empty @14:17:10.27 5.8psi after compress @14:17:46.86 13.1psi after compress (stable) @14:17:46.90 Archive.in :gulp @14:17:47.02 Archive.seek :topVent @14:17:53.36 15.0psi after gulp @14:17:58.96 14.8psi after gulp (stable) @14:17:59.06 Archive stroke #2 of 8 @14:17:59.32 Archive.in :pressurize @14:17:59.41 Archive.seek :pressurized @14:18:11.88 16.1psi after pressurize @14:18:11.91 Archive.in :compress @14:18:12.02 Archive.seek :empty @14:18:27.79 19.7psi after compress @14:18:51.12 Reached 25.3 of 25.3psi after pumping 3460uL (2.00 strokes) @14:18:51.14 Archive.in :default @14:18:51.26 Archive.hold @14:18:51.37 Handoff.select :waste @14:18:51.53 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:18:58.16 delay 1:00 @14:19:58.19 Handoff.select :blocked @14:19:58.38 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:20:20.97 Handoff.select :waste @14:20:21.23 3.2psi at evacEnd @14:20:21.26 Air.seek :fullVent @14:20:22.85 Archive.in :fast @14:20:22.93 Archive.seek :topVent @14:20:30.14 Seawater evacuated, Pressure above/below filter released. @14:20:30.28 Lysis1.in :slow @14:20:30.37 Lysis1.seek 1050uL @14:21:00.05 delay 5 seconds @14:21:05.08 Handoff.select :blocked @14:21:05.31 Lysis1.seek 450uL @14:22:04.51 delay 3 seconds @14:22:07.60 Air.in mix with 1.26318 parts Lysis1 @14:22:08.40 Air.seek :evac @14:22:08.45 Lysis1.seek 300uL @14:22:23.45 Lysis1.seek :empty @14:22:53.18 Lysis buffer delivered @37.7psi @14:22:53.20 Polling Can every 2:00 @14:22:53.78 PuckHeater.configure :default #ambient 21.2C @14:22:53.87 PuckHeater.seek 79.8C @14:23:45.39 PuckHeater.hold 2:00,79.8C @14:25:45.27 Polling Can every 2:00 @14:25:45.82 PuckHeater.configure :default #ambient 21.2C @14:25:45.92 PuckHeater.hold 30 seconds,78.7C @14:26:16.10 Polling Can every 2:00 @14:26:16.67 PuckHeater.configure :default #ambient 21.2C @14:26:16.77 PuckHeater.hold 30 seconds,78.2C @14:26:46.95 Polling Can every 2:00 @14:26:47.50 PuckHeater.configure :default #ambient 21.2C @14:26:47.59 PuckHeater.hold 10:00,77.7C @14:36:46.72 Lysis complete. Syringe pressure @29.9psi @14:36:46.84 Archive syringe pumping up to 5 strokes to reach 40psi @14:36:46.87 Archive stroke #1 of 5 @14:36:46.99 Archive.in :pressurize @14:36:47.07 Archive.seek :pressurized @14:36:59.54 29.4psi after pressurize @14:37:05.63 28.4psi after pressurize (stable) @14:37:05.66 Archive.in :compress @14:37:05.77 Archive.seek :empty @14:37:13.10 Reached 40.8 of 40.0psi after pumping 1550uL (0.90 strokes) @14:37:13.12 Archive.in :default @14:37:13.28 Archive.hold @14:37:13.46 delay 2 seconds @14:37:15.49 Archive.in :fast @14:37:15.57 Archive.seek :full @14:37:22.21 Lysis1.seek 16uL @14:37:23.91 delay 1 second @14:37:24.95 Archive.seek :full @14:37:25.05 Lysis1.seek :empty @14:37:26.83 Handoff.select :blocked @14:37:27.03 Initial air pressure @47.0psi @14:37:27.12 Lysis1.in :extract @14:37:27.24 Lysis1.seek 600uL,nil @14:37:29.70 Archive syringe pumping up to 6 strokes to reach 25psi @14:37:29.72 Archive stroke #1 of 6 @14:37:29.88 Archive.in :pressurize @14:37:30.01 Archive.seek :pressurized @14:37:33.96 Archive.in :default @14:37:34.60 Archive.hold @14:37:35.09 Initial 600uL lysate @14.3psi @14:37:35.11 Handoff.select :blocked @14:37:35.26 Initial air pressure @13.5psi @14:37:35.35 Lysis1.seek 825uL,nil @14:37:35.51 Archive syringe pumping up to 6 strokes to reach 17psi @14:37:35.54 Archive stroke #1 of 6 @14:37:35.77 Archive.in :pressurize @14:37:35.89 Archive.seek :pressurized @14:37:43.74 14.0psi after pressurize @14:37:49.83 13.2psi after pressurize (stable) @14:37:49.86 Archive.in :compress @14:37:50.04 Archive.seek :empty @14:37:51.90 Reached 17.1 of 17.0psi after pumping 1429uL (0.83 strokes) @14:37:51.93 Archive.in :default @14:37:52.06 Archive.hold @14:37:53.39 Syringe::LysatePlungerStuck in PROCESSING -- at < 630uL during Lysis Syringe #1 (LyS1) move @14:37:53.45 Lysis1.in :default @14:37:53.55 Lysis1.seek 595uL @14:37:56.29 Lysis1.in :extract @14:37:56.38 Lysis1.seek 825uL,nil @14:37:58.18 Syringe::LysatePlungerStuck in PROCESSING -- at < 611uL during Lysis Syringe #1 (LyS1) move @14:37:58.24 Lysis1.in :default @14:37:58.34 Lysis1.seek 576uL @14:38:01.25 Lysis1.in :extract @14:38:01.35 Lysis1.seek 825uL,nil @14:38:03.14 Syringe::LysatePlungerStuck in PROCESSING -- at < 595uL during Lysis Syringe #1 (LyS1) move @14:38:03.21 Lysis1.in :default @14:38:03.33 Lysis1.seek 560uL @14:38:06.22 Lysis1.in :extract @14:38:06.31 Lysis1.seek 825uL,nil @14:38:08.55 Syringe::LysatePlungerStuck in PROCESSING -- at < 565uL during Lysis Syringe #1 (LyS1) move @14:38:08.60 Lysis1.in :default @14:38:08.72 Lysis1.seek 530uL @14:38:12.22 Lysis1.in :extract @14:38:12.30 Lysis1.seek 825uL,nil @14:38:14.13 Syringe::LysatePlungerStuck in PROCESSING -- at < 551uL during Lysis Syringe #1 (LyS1) move @14:38:14.20 Lysis1.in :default @14:38:14.31 Lysis1.seek 516uL @14:38:17.22 Lysis1.in :extract @14:38:17.31 Lysis1.seek 825uL,nil @14:38:19.12 Syringe::LysatePlungerStuck in PROCESSING -- at < 535uL during Lysis Syringe #1 (LyS1) move @14:38:19.17 Lysis1.in :default @14:38:19.27 Lysis1.seek 500uL @14:38:22.14 Lysis1.in :extract @14:38:22.22 Lysis1.seek 825uL,nil @14:38:23.86 Syringe::LysatePlungerStuck in PROCESSING -- at < 506uL during Lysis Syringe #1 (LyS1) move @14:38:23.90 Lysis1.in :default @14:38:24.01 Lysis1.seek 471uL @14:38:27.45 Lysis1.in :extract @14:38:27.66 Lysis1.seek 825uL,nil @14:38:29.42 Syringe::LysatePlungerStuck in PROCESSING -- at < 485uL during Lysis Syringe #1 (LyS1) move @14:38:29.49 Lysis1.in :default @14:38:29.59 Lysis1.seek 450uL @14:38:32.45 Lysis1.in :extract @14:38:32.54 Lysis1.seek 825uL,nil @14:38:34.19 Syringe::LysatePlungerStuck in PROCESSING -- at < 457uL during Lysis Syringe #1 (LyS1) move @14:38:34.23 Lysis1.in :default @14:38:34.35 Lysis1.seek 421uL @14:38:37.18 Lysis1.in :extract @14:38:37.28 Lysis1.seek 825uL,nil @14:38:39.43 Syringe::LysatePlungerStuck in PROCESSING -- at < 488uL during Lysis Syringe #1 (LyS1) move @14:38:39.48 Lysis1.in :default @14:38:39.58 Lysis1.seek 453uL @14:38:42.41 Lysis1.in :extract @14:38:42.49 Lysis1.seek 825uL,nil @14:38:44.21 Syringe::LysatePlungerStuck in PROCESSING -- at < 462uL during Lysis Syringe #1 (LyS1) move @14:38:45.07 Lysis1.in :default @14:38:45.18 Lysis1.seek 427uL @14:38:48.97 Lysis1.in :extract @14:38:49.06 Lysis1.seek 825uL,nil @14:38:50.66 Syringe::LysatePlungerStuck in PROCESSING -- at < 434uL during Lysis Syringe #1 (LyS1) move @14:38:50.72 Lysis1.in :default @14:38:50.84 Lysis1.seek 398uL @14:38:53.69 Lysis1.in :extract @14:38:53.79 Lysis1.seek 825uL,nil @14:38:55.42 Syringe::LysatePlungerStuck in PROCESSING -- at < 404uL during Lysis Syringe #1 (LyS1) move @14:38:55.47 Lysis1.in :default @14:38:55.57 Lysis1.seek 369uL @14:38:58.38 Lysis1.in :extract @14:38:58.46 Lysis1.seek 825uL,nil @14:39:00.21 Syringe::LysatePlungerStuck in PROCESSING -- at < 380uL during Lysis Syringe #1 (LyS1) move @14:39:00.27 Lysis1.in :default @14:39:00.37 Lysis1.seek 345uL @14:39:03.25 Lysis1.in :extract @14:39:03.35 Lysis1.seek 825uL,nil @14:39:05.24 Syringe::LysatePlungerStuck in PROCESSING -- at < 345uL during Lysis Syringe #1 (LyS1) move @14:39:05.29 Lysis1.in :default @14:39:05.40 Lysis1.seek 310uL @14:39:08.58 Lysis1.in :extract @14:39:08.68 Lysis1.seek 825uL,nil @14:39:10.46 Syringe::LysatePlungerStuck in PROCESSING -- at < 323uL during Lysis Syringe #1 (LyS1) move @14:39:10.51 Lysis1.in :default @14:39:10.63 Lysis1.seek 288uL @14:39:13.50 Lysis1.in :extract @14:39:13.66 Lysis1.seek 825uL,nil @14:39:15.69 Archive syringe pumping up to 6 strokes to reach 17psi @14:39:15.71 Archive stroke #1 of 6 @14:39:15.92 Archive.in :compress @14:39:16.03 Archive.seek :empty @14:39:19.30 Reached 17.2 of 17.0psi after pumping 1492uL (0.86 strokes) @14:39:19.32 Archive.in :default @14:39:19.43 Archive.hold @14:39:19.97 Recovered 825uL lysate @16.5psi @14:39:20.00 Air.in :halfSpeed @14:39:20.13 Air.seek :fullVent @14:39:23.38 Puck Top Vented, bottom @11.1psi @14:39:23.42 Handoff.select :waste @14:39:23.60 delay 5 seconds @14:39:28.68 Handoff open to waste, bottom @0.1psi @14:39:28.71 Air.in :default @14:39:28.81 Air.seek :evac @14:39:30.54 Handoff.select :close @14:39:30.74 Gate.power :heat,:OFF @14:39:30.76 Gate.power :cartridge,:OFF @14:39:30.90 PinPort.in :unlocking @14:39:30.98 PinPort.seek :clear @14:39:31.46 Carousel.in :default @14:39:31.61 Carousel.home.seek 6 @14:39:31.97 Plunge.seek :locked @14:39:32.90 Archive.in :unlocking @14:39:33.04 Archive.seek :clear @14:39:33.18 Air.in :unlocking @14:39:33.33 Air.seek :clear @14:39:33.47 Lysis1.in :unlocking @14:39:33.65 Lysis1.seek :clear @14:39:33.75 Lysis2.in :unlocking @14:39:33.87 Lysis2.seek :clear @14:39:38.51 Gate.power :analytic1,:OFF @14:39:38.53 Gate.power :motor,:OFF @14:39:38.59 Gate.power :core,:OFF @14:39:38.63 Gate.power :main,:OFF @14:39:38.66 Safely stopped and ready to power off @14:39:59.62 -> Cartridge.unlock @14:39:59.70 BusOff in preston -- core bus was not powered while reading Handoff Pin Port (PP)'s status @14:40:05.73 -> ESP.reready! @14:40:05.78 Gate.power :sampler,:OFF,limit to 3500mA @14:40:05.83 Gate.power :skimmer,:OFF,limit to 3500mA @14:40:05.87 Gate.power :main,:ON,limit to 5000mA @14:40:05.91 Gate.power :analytic1,:ON,limit to 4000mA @14:40:06.03 Powering core bus @14:40:08.81 Plunge.configure :default @14:40:08.93 SamplePump.configure :default @14:40:09.03 Toroid.configure :default @14:40:09.15 Carousel.configure :default @14:40:09.27 Abs.configure :default @14:40:09.37 PhaseSeparator.configure :default @14:40:09.48 Archive.configure :default @14:40:09.60 Air.configure :default @14:40:09.69 Lysis1.configure :default @14:40:10.03 Lysis2.configure :default @14:40:10.17 PinPort.configure :default @14:40:10.27 All elf controllers running firmware version 4.92 @14:40:10.30 Gate.power :motor,:ON,limit to 5000mA @14:40:13.29 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @14:40:13.32 AnalyticModule::Unknown in preston -- SPR @14:40:13.39 Plunge.home.jog 0 @14:40:13.45 Toroid.home.jog 0 @14:40:13.60 Carousel.home.jog 0 @14:40:13.70 Archive.home.jog 0 @14:40:13.84 Air.home.jog 0 @14:40:13.92 Lysis1.home.jog 0 @14:40:14.17 Lysis2.home.jog 0 @14:40:14.23 PinPort.home.jog 0 @14:40:15.42 Plunge.in :homing @14:40:15.77 Plunge.jog 1608 @14:40:16.19 Air.in :homing @14:40:16.25 Archive.in :homing @14:40:16.30 Toroid.in :homing @14:40:16.36 Lysis1.in :homing @14:40:16.59 Archive.jog 1406 @14:40:16.63 Air.jog 1371 @14:40:16.68 Toroid.jog 631 @14:40:16.74 Lysis1.jog 1395 @14:40:17.13 Lysis2.in :homing @14:40:17.17 PinPort.in :homing @14:40:17.30 Lysis2.jog 1406 @14:40:17.34 PinPort.jog -105 @14:40:17.56 Plunge.setRawPosition! 50 @14:40:17.63 Plunge.seek :home @14:40:17.77 PinPort.setRawPosition! 0 @14:40:17.84 PinPort.seek :home @14:40:18.00 Archive.setRawPosition! 50 @14:40:18.05 PinPort.in :default @14:40:18.16 Plunge.in :default @14:40:18.34 Archive.seek :home @14:40:18.48 Lysis1.setRawPosition! 50 @14:40:18.53 Air.setRawPosition! 50 @14:40:18.68 Lysis1.seek :home @14:40:18.72 Air.seek :home @14:40:18.95 Lysis2.setRawPosition! 50 @14:40:18.97 Archive.in :default @14:40:19.15 Lysis2.seek :home @14:40:19.32 Air.in :default @14:40:19.35 Toroid.setRawPosition! 0 @14:40:19.40 Lysis1.in :default @14:40:19.59 Toroid.seek :home @14:40:19.75 Toroid.in :default @14:40:19.78 Lysis2.in :default @14:40:20.21 Toroid.seek :clear @14:40:21.95 Carousel.in :default @14:40:22.13 Carousel.home.seek 6 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 6 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:42:51.45 -> slot 6=>[:dry,:lyseHold_phyto] {6 => { Type: :lyseHold_phyto, State: :dry}} @14:42:58.96 -> fire lyseHold_phyto # @14:42:59.98 Toroid.seek :clear @14:43:00.65 Carousel.in :default @14:43:00.91 Carousel.home.seek 6 @14:43:01.20 Selecting Cartridge 6 @14:43:01.53 Carousel.seek 6 @14:43:01.82 Lysis1.in :fast @14:43:01.89 Lysis1.seek :locked @14:43:04.57 Plunge.seek :locked @14:43:04.76 Air.in :fast @14:43:04.87 Air.seek :locked @14:43:05.87 Carousel.reconfigure :locked @14:43:06.06 Powering cartridge bus @14:43:08.72 Gate.setIR 1750,5000,0,6 @14:43:08.86 Gate.setIR 0,5000,0,6 @14:43:08.95 PuckHeater.configure :default #ambient 21.2C @14:43:09.05 Gate.power :heat,:ON,limit to 5000mA @14:43:09.76 Mounted lysis Cartridge board#Q05061 @14:43:09.83 Using this cartridge's heat loss curve for HeatingAccelInCarousel @14:43:09.90 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 6" @14:43:10.35 Preparing to filter cartridge 6 @14:43:10.74 Air.in :default @14:43:10.87 Bypass.open @14:43:11.06 Air.seek :sealed @14:43:11.77 Toroid.in :hold @14:43:11.99 Toroid.seek :crack @14:43:13.36 upsync @14:43:13.75 Toroid.seek :cartridge @14:43:14.90 Toroid.in :default @14:43:14.99 Toroid.seek :clear @14:43:16.83 Exhaust.open @14:43:17.54 Intake.open @14:43:18.33 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:43:20.95 Vacuum tare is 52% of full scale at 21C (drifted 1%) @14:43:21.24 Pressurizing cartridge to 26psi above 15.5psia @14:43:21.26 SamplePump.setPosition! 0ml @14:43:21.36 Bypass.close @14:43:22.04 Exhaust.close @14:43:22.81 Abs.in :default @14:43:22.89 Abs.hold @14:43:23.16 SamplePump.in :charge @14:43:23.27 SamplePump.seek 2.3ml,42 seconds @14:43:37.56 Abs.stop @14:43:37.66 Intake.close @14:43:38.45 Pressurized cartridge with 0.915ml to 27.9psi @14:43:38.47 Isolated @38.4psia (22.8psi) @14:43:38.49 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @14:43:38.59 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:43:43.19 Cartridge pressure fell 1% to 38.0psia @14:43:43.32 Sampling 50.0ml @14:43:43.34 SamplePump.setPosition! 0ml @14:43:43.45 Exhaust.open @14:43:44.15 Intake.open @14:43:45.06 Abs.in :default @14:43:45.15 Abs.hold @14:43:45.51 SamplePump.in :start @14:43:45.61 SamplePump.seek 50ml,4:40 @14:43:46.20 SamplePump.configure :sample @14:43:48.61 Cartridge 6 filtering 2/50ml at 0.44ml/s with 29.2psi vacuum @14:44:48.66 Cartridge 6 filtering 39/50ml at 0.61ml/s with 31.6psi vacuum @14:45:07.42 Abs.stop @14:45:07.61 Intake.close @14:45:07.72 Exhaust.close @14:45:07.97 Toroid.in :hold @14:45:08.06 Toroid.seek :bypass @14:45:09.78 Toroid.in :default @14:45:09.87 Toroid.seek :clear @14:45:11.52 Sampled 50.0ml @14:45:12.61 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 6" @14:45:13.13 Handoff.select :waste @14:45:13.31 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:45:15.76 upsync @14:45:19.95 Syringe tare is 52% of full scale at 21C (drifted 1%) @14:45:19.99 PinPort.in :locking @14:45:20.09 PinPort.seek :locked @14:45:20.62 Archive.in :fast @14:45:20.70 Lysis2.in :fast @14:45:20.81 Lysis1.seek 1350uL @14:45:20.83 Archive.seek :full @14:45:21.03 Lysis2.seek :empty @14:45:29.16 Plunge.seek :reagent @14:45:30.17 Evacuating puck with filter bubble point of 24psi @14:45:30.21 PinPort.seek :locked @14:45:30.36 Handoff.select :waste @14:45:30.40 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:45:37.00 Air syringe pumping up to 1 strokes to reach 4psi @14:45:37.02 Air stroke #1 of 1 @14:45:37.16 Air.in :pressurize @14:45:37.24 Air.seek :pressurized @14:45:42.89 0.7psi after pressurize @14:45:42.92 Air.in :compress @14:45:43.04 Air.seek :evac @14:45:46.66 0.1psi after compress @14:45:57.30 0.1psi after compress (stable) @14:45:57.32 Air.in :default @14:45:57.43 Air.hold @14:45:57.67 at 0.1psi Opened to waste @14:45:57.69 Handoff.select :blocked @14:45:57.87 Archive syringe pumping up to 8 strokes to reach 24psi @14:45:57.88 Archive stroke #1 of 8 @14:45:58.04 Archive.in :pressurize @14:45:58.18 Archive.seek :pressurized @14:46:10.64 0.8psi after pressurize @14:46:16.23 0.2psi after pressurize (stable) @14:46:16.27 Archive.in :compress @14:46:16.39 Archive.seek :empty @14:46:32.17 1.2psi after compress @14:46:42.76 0.1psi after compress (stable) @14:46:42.89 Archive.in :gulp @14:46:43.01 Archive.seek :topVent @14:46:49.35 0.8psi after gulp @14:46:54.94 0.2psi after gulp (stable) @14:46:54.96 Archive stroke #2 of 8 @14:46:55.12 Archive.in :pressurize @14:46:55.21 Archive.seek :pressurized @14:47:07.68 0.9psi after pressurize @14:47:13.27 0.1psi after pressurize (stable) @14:47:13.30 Archive.in :compress @14:47:13.40 Archive.seek :empty @14:47:29.15 1.4psi after compress @14:47:39.74 0.1psi after compress (stable) @14:47:39.77 Archive.in :gulp @14:47:39.90 Archive.seek :topVent @14:47:46.25 0.8psi after gulp @14:47:51.84 0.2psi after gulp (stable) @14:47:51.95 Archive stroke #3 of 8 @14:47:52.12 Archive.in :pressurize @14:47:52.23 Archive.seek :pressurized @14:48:04.69 0.9psi after pressurize @14:48:10.28 0.1psi after pressurize (stable) @14:48:10.32 Archive.in :compress @14:48:10.43 Archive.seek :empty @14:48:26.21 1.4psi after compress @14:48:36.80 0.1psi after compress (stable) @14:48:36.83 Archive.in :gulp @14:48:36.96 Archive.seek :topVent @14:48:43.30 0.9psi after gulp @14:48:49.87 0.2psi after gulp (stable) @14:48:50.00 Archive stroke #4 of 8 @14:48:50.18 Archive.in :pressurize @14:48:50.28 Archive.seek :pressurized @14:49:02.74 1.0psi after pressurize @14:49:08.34 0.3psi after pressurize (stable) @14:49:08.37 Archive.in :compress @14:49:08.49 Archive.seek :empty @14:49:21.60 Archive.in :default @14:49:21.70 Archive.hold @14:49:21.82 Handoff.select :blocked @14:49:21.94 Archive.setRawPosition! -8095 @14:49:22.04 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 56uL (sensed 81uL) @14:49:22.07 Waiting to Resume from Checkpoint "Archive Syringe (ArS) positionErr at 56uL (sensed 81uL)" @14:49:22.15 BadNews.email "Archive Syringe (ArS) positionErr at 56uL (sensed 81uL)",Subject: "Slide::Error in PROCESSING" @14:49:25.06 upsync @14:49:56.58 -> Syringe.psi 0.321603893547888 @14:51:39.64 -> PP.disconnect @14:51:39.67 PinPort.in :unlocking @14:51:39.79 PinPort.seek :clear Handoff Pin Port (PP) clear @14:52:00.05 -> ArS.jog -100 @14:52:00.11 Archive.jog -100 @14:52:00.94 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 55uL (sensed 78uL) while reading Archive Syringe (ArS)'s status @14:52:19.19 -> PP.connect @14:52:19.24 PinPort.in :locking @14:52:19.34 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:52:22.87 -> Syringe.psi 0.17026088481947 @14:52:55.82 -> PP.connect @14:52:55.87 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:53:03.90 -> ArS.jog -100 @14:53:03.95 Archive.jog -100 @14:53:04.71 Slide::Error in preston -- Archive Syringe (ArS) Not yet Homed! @14:53:35.93 Session Begun @14:53:38.39 -> threads [#, #, #, #, #, #, #, #, #, #, #, #, #] @14:53:49.86 -> #hi Brent! @14:54:10.05 -> #good afternoon @14:54:12.02 -> #Honu is being a problem child, this is nadia @14:54:27.81 -> #Yes, I see the carnage @14:54:36.08 -> #What are the issues? @14:55:32.32 -> #Anything I can help with? I'm free for about an hour @14:56:22.58 -> #the cartridge isn't able to release pressure now. We are working through isolating the fluidic connections. It seems to be a handoff issue. When engaging the PP. I see no pressure, but the Archive syringe is stalling because the pressure in the cartridge. @14:56:54.49 -> #I think I have a handle on honu for now, but trying to run betty to run calcar but the SC is angry. @14:57:07.95 -> #Doesn't sound like I can be much use. Sorry :-( @14:57:46.61 -> #Running calcar on betty is a good idea. @14:58:05.09 -> #would you mind checking betty out real quick. I can't ready the SC for some reason. @14:58:13.41 -> PP.connect @14:58:13.49 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:58:18.36 -> Handoff.waste @14:58:19.03 Handoff.select :waste Handoff Valve::Manifold selects waste @14:58:23.40 -> #I'll to play with Betty then. @14:58:32.02 -> #thanks brent! @14:58:37.90 -> ArS.jog -100 @14:58:37.95 Archive.jog -100 @14:58:38.73 Slide::Error in preston -- Archive Syringe (ArS) Not yet Homed! @14:58:50.25 -> Cartridge.unlock @14:58:50.32 Gate.power :heat,:OFF @14:58:50.35 Gate.power :cartridge,:OFF @14:58:50.51 PinPort.in :unlocking @14:58:50.62 PinPort.seek :clear @14:58:51.13 Carousel.in :default @14:58:51.30 Carousel.home.seek 6 @14:58:51.69 Slide::Error in preston -- Archive Syringe (ArS) Not yet Homed! @14:59:03.42 -> ESP.reready! @14:59:03.50 Plunge.in :default @14:59:03.66 Toroid.in :default @14:59:03.76 Carousel.in :default @14:59:03.86 Archive.in :default @14:59:03.92 Air.in :default @14:59:04.02 Plunge.home.jog 0 @14:59:04.15 Lysis1.in :default @14:59:04.29 Lysis2.in :default @14:59:04.37 PinPort.in :default @14:59:04.47 Toroid.home.jog 0 @14:59:04.71 Carousel.home.jog 0 @14:59:04.83 Archive.home.jog 0 @14:59:04.89 Air.home.jog 0 @14:59:05.03 Lysis1.home.jog 0 @14:59:05.10 Lysis2.home.jog 0 @14:59:05.21 PinPort.home.jog 0 @14:59:06.01 Plunge.in :homing @14:59:06.45 Plunge.jog 2331 @14:59:06.53 Lysis2.in :homing @14:59:06.60 Air.in :homing @14:59:06.65 Toroid.in :homing @14:59:06.73 PinPort.in :homing @14:59:06.77 Lysis1.in :homing @14:59:06.85 Archive.in :homing @14:59:07.23 Lysis2.jog 9845 @14:59:07.32 Air.jog 9020 @14:59:07.38 Toroid.jog 619 @14:59:07.48 PinPort.jog -105 @14:59:07.54 Lysis1.jog 3317 @14:59:07.60 Archive.jog 9457 @14:59:08.14 PinPort.setRawPosition! 0 @14:59:08.24 PinPort.seek :home @14:59:08.39 PinPort.in :default @14:59:08.55 Plunge.setRawPosition! 50 @14:59:08.65 Plunge.seek :home @14:59:08.97 Plunge.in :default @14:59:09.94 Toroid.setRawPosition! 0 @14:59:10.06 Lysis1.setRawPosition! 50 @14:59:10.13 Toroid.seek :home @14:59:10.34 Lysis1.seek :home @14:59:10.48 Toroid.in :default @14:59:10.67 Lysis1.in :default @14:59:12.47 Air.setRawPosition! 50 @14:59:12.56 Air.seek :home @14:59:12.80 Lysis2.setRawPosition! 50 @14:59:13.40 Lysis2.seek :home @14:59:13.67 Archive.setRawPosition! 50 @14:59:13.70 Air.in :default @14:59:13.86 Archive.seek :home @14:59:14.03 Lysis2.in :default @14:59:14.22 Archive.in :default @14:59:14.60 Toroid.seek :clear @14:59:16.34 Carousel.in :default @14:59:16.52 Carousel.home.seek 6 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) at 50ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 6 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:59:29.03 -> ArS.goHome! @14:59:29.09 Archive.in :default @14:59:29.21 Archive.home.jog 0 @14:59:29.43 Archive.in :homing @14:59:29.54 Archive.jog 9278 @14:59:34.45 Archive.setRawPosition! 50 @14:59:34.55 Archive.seek :home @14:59:34.88 Archive.in :default Archive Syringe (ArS) clear @15:15:26.67 Session Ended @16:01:04.89 -> Handoff.waste @16:01:04.91 Handoff.select :waste Handoff Valve::Manifold selects waste @16:01:09.84 -> Handoff.blocked @16:01:09.86 Handoff.select :blocked Handoff Valve::Manifold selects blocked @16:01:13.34 -> Handoff.waste @16:01:13.37 Handoff.select :waste Handoff Valve::Manifold selects waste @16:01:15.54 -> Handoff.blocked @16:01:15.58 Handoff.select :blocked Handoff Valve::Manifold selects blocked @16:01:34.29 -> delay 5;Handoff.waste;delay 2;Handoff.blocked @16:01:34.33 delay 5 seconds @16:01:39.37 Handoff.select :waste @16:01:39.53 delay 2 seconds @16:01:41.55 Handoff.select :blocked Handoff Valve::Manifold selects blocked @16:57:41.43 -> SC.to 1 @16:57:41.47 Carousel.seek 1 Storage Carousel (SC) at 1 @16:57:59.63 -> PP.connect @16:57:59.68 Cartridge::TypeUnknown in preston -- No cartridge is mounted @16:58:08.99 -> PP.jog 100 @16:58:09.04 PinPort.jog 100 Handoff Pin Port (PP) at washStation + 23 counts @16:58:14.76 -> PP.cartridge @16:58:14.80 Cartridge::TypeUnknown in preston -- No cartridge is mounted @16:58:29.24 -> PP.clear @16:58:29.28 PinPort.in :unlocking @16:58:29.40 PinPort.seek :clear Handoff Pin Port (PP) clear @16:58:32.88 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2085 => ["cartridge",:end,:insert,:inserted,:locked]} @16:58:52.66 -> PP.jog 2085 @16:58:52.71 PinPort.in :default @16:58:52.77 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @16:59:31.29 -> PP.clear @16:59:31.33 PinPort.in :unlocking @16:59:31.43 PinPort.seek :clear Handoff Pin Port (PP) clear @16:59:37.02 -> SC.to 5 @16:59:37.05 Carousel.seek 5 Storage Carousel (SC) at 5 @16:59:42.74 -> PP.jog 2085 @16:59:42.78 PinPort.in :default @16:59:42.87 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @16:59:54.12 -> PP.clear @16:59:54.20 PinPort.in :unlocking @16:59:54.28 PinPort.seek :clear Handoff Pin Port (PP) clear @17:00:00.42 -> SC.to 10 @17:00:00.46 Carousel.seek 10 Storage Carousel (SC) at 10 @17:00:06.58 -> PP.jog 2085 @17:00:06.62 PinPort.in :default @17:00:06.72 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:00:24.90 -> PP.clear @17:00:24.96 PinPort.in :unlocking @17:00:25.05 PinPort.seek :clear Handoff Pin Port (PP) clear @17:00:28.84 -> SC.to 15 @17:00:28.90 Carousel.seek 15 Storage Carousel (SC) at 15 @17:00:32.96 -> PP.jog 2085 @17:00:33.00 PinPort.in :default @17:00:33.12 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:00:41.97 -> PP.clear @17:00:42.02 PinPort.in :unlocking @17:00:42.15 PinPort.seek :clear Handoff Pin Port (PP) clear @17:00:45.28 -> SC.to 20 @17:00:45.32 Carousel.seek 20 Storage Carousel (SC) at 20 @17:00:50.53 -> PP.jog 2085 @17:00:50.56 PinPort.in :default @17:00:50.66 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:00:57.30 -> PP.clear @17:00:57.33 PinPort.in :unlocking @17:00:57.43 PinPort.seek :clear Handoff Pin Port (PP) clear @17:01:02.94 -> SC.to 25 @17:01:02.97 Carousel.seek 25 Storage Carousel (SC) at 25 @17:01:09.13 -> PP.jog 2085 @17:01:09.17 PinPort.in :default @17:01:09.28 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:01:15.45 -> PP.clear @17:01:15.50 PinPort.in :unlocking @17:01:15.58 PinPort.seek :clear Handoff Pin Port (PP) clear @17:01:21.01 -> SC.to 30 @17:01:21.06 Carousel.seek 30 Storage Carousel (SC) at 30 @17:01:31.46 -> PP.jog 2085 @17:01:31.50 PinPort.in :default @17:01:31.59 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:01:38.88 -> PP.clear @17:01:38.91 PinPort.in :unlocking @17:01:39.02 PinPort.seek :clear Handoff Pin Port (PP) clear @17:01:41.62 -> SC.to 35 @17:01:41.66 Carousel.seek 35 Storage Carousel (SC) at 35 @17:01:45.30 -> PP.jog 2085 @17:01:45.34 PinPort.in :default @17:01:45.44 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:01:55.33 -> PP.clear @17:01:55.37 PinPort.in :unlocking @17:01:55.47 PinPort.seek :clear Handoff Pin Port (PP) clear @17:02:02.71 -> SC.to 15 @17:02:02.77 Carousel.seek 15 Storage Carousel (SC) at 15 @17:02:10.89 -> PP.jog 2085 @17:02:10.93 PinPort.in :default @17:02:11.03 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:02:23.71 -> PP.clear @17:02:23.75 PinPort.in :unlocking @17:02:23.82 PinPort.seek :clear Handoff Pin Port (PP) clear @17:02:30.08 -> SC.to 40 @17:02:30.18 Carousel.seek 40 Storage Carousel (SC) at 40 @17:02:41.91 -> PP.jog 2085 @17:02:41.96 PinPort.in :default @17:02:42.03 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:02:55.26 -> PP.clear @17:02:55.32 PinPort.in :unlocking @17:02:55.40 PinPort.seek :clear Handoff Pin Port (PP) clear @17:03:09.86 -> SC.to 45 @17:03:09.92 Carousel.seek 45 Storage Carousel (SC) at 45 @17:03:13.86 -> PP.jog 208 @17:03:13.89 PinPort.in :default @17:03:14.01 PinPort.jog 208 Handoff Pin Port (PP) at washStation + 131 counts @17:03:17.93 -> PP.clear @17:03:17.97 PinPort.in :unlocking @17:03:18.07 PinPort.seek :clear Handoff Pin Port (PP) clear @17:03:21.83 -> PP.jog 2085 @17:03:21.86 PinPort.in :default @17:03:21.96 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:03:29.75 -> PP.clear @17:03:29.79 PinPort.in :unlocking @17:03:29.89 PinPort.seek :clear Handoff Pin Port (PP) clear @17:03:37.42 -> SC.to 50 @17:03:37.47 Carousel.seek 50 Storage Carousel (SC) at 50 @17:03:40.79 -> PP.clear @17:03:40.93 PinPort.seek :clear Handoff Pin Port (PP) clear @17:03:44.62 -> PP.jog 2085 @17:03:44.65 PinPort.in :default @17:03:44.75 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:03:51.70 -> PP.clear @17:03:51.76 PinPort.in :unlocking @17:03:51.84 PinPort.seek :clear Handoff Pin Port (PP) clear @17:03:56.72 -> SC.to 55 @17:03:56.78 Carousel.seek 55 Storage Carousel (SC) at 55 @17:04:00.18 -> PP.jog 2085 @17:04:00.21 PinPort.in :default @17:04:00.31 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:04:06.77 -> PP.clear @17:04:06.83 PinPort.in :unlocking @17:04:06.91 PinPort.seek :clear Handoff Pin Port (PP) clear @17:04:10.92 -> SC.to 60 @17:04:10.96 Carousel.seek 60 Storage Carousel (SC) at 60 @17:04:14.82 -> PP.jog 2085 @17:04:14.85 PinPort.in :default @17:04:14.95 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:04:30.98 -> PP.clear @17:04:31.02 PinPort.in :unlocking @17:04:31.13 PinPort.seek :clear Handoff Pin Port (PP) clear @17:04:44.44 -> SC.to 15 @17:04:44.49 Carousel.seek 15 Storage Carousel (SC) at 15 @17:05:03.40 -> PV.reagent @17:05:03.45 Plunge.seek :reagent Plunge Valve (PV) at reagentLG @17:05:10.32 -> PP.jog 2085 @17:05:10.35 PinPort.in :default @17:05:10.46 PinPort.jog 2085 Handoff Pin Port (PP) cartridge @17:05:18.16 -> Cartridge.lock @17:05:18.35 Lysis1.in :fast @17:05:18.44 Lysis1.seek :locked @17:05:21.20 Air.in :fast @17:05:21.29 Air.seek :locked @17:05:22.29 Carousel.reconfigure :locked @17:05:22.48 Powering cartridge bus @17:05:25.15 Gate.setIR 1750,5000,0,6 @17:05:25.28 Gate.setIR 0,5000,0,6 @17:05:25.38 PuckHeater.configure :default #ambient 21.2C @17:05:25.47 Gate.power :heat,:ON,limit to 5000mA @17:05:26.21 Mounted lysis Cartridge board#Q05067 @17:05:26.27 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @17:05:33.75 -> Handoff.waste @17:05:33.79 Handoff.select :waste Handoff Valve::Manifold selects waste @17:05:38.14 -> PV.reagent @17:05:38.20 Plunge.seek :reagent Plunge Valve (PV) at reagentLG @17:06:25.92 -> Air.evac @17:06:25.96 Air.in :default @17:06:26.06 Air.seek :evac Air Syringe (AirS) at evac @17:07:21.90 -> LyS1.empty @17:07:21.96 Lysis1.in :default @17:07:22.05 Lysis1.seek :empty Lysis Syringe #1 (LyS1) empty @17:07:52.50 -> LyS1.clear @17:07:52.54 Lysis1.seek :clear Lysis Syringe #1 (LyS1) clear @17:08:10.44 -> Cartridge.unlock @17:08:10.49 Gate.power :heat,:OFF @17:08:10.51 Gate.power :cartridge,:OFF @17:08:10.63 PinPort.in :unlocking @17:08:10.73 PinPort.seek :clear @17:08:11.21 Carousel.in :default @17:08:11.36 Carousel.home.seek 15 @17:08:11.83 Air.in :unlocking @17:08:11.98 Air.seek :clear @17:08:16.29 Plunge.in :unlocking @17:08:16.38 Plunge.seek :clear @08:00:20.42PDT18-Jul-24 -> to 14 @08:00:20.75 Carousel.seek 14 @08:00:21.30 Lysis1.in :fast @08:00:21.39 Lysis1.seek :locked @08:00:24.05 Plunge.in :default @08:00:24.20 Plunge.seek :locked @08:00:24.35 Air.in :fast @08:00:24.43 Air.seek :locked @08:00:25.42 Carousel.reconfigure :locked @08:00:25.61 Powering cartridge bus @08:00:28.27 Gate.setIR 1750,5000,0,6 @08:00:29.01 Gate.setIR 0,5000,0,6 @08:00:29.18 PuckHeater.configure :default #ambient 21.2C @08:00:29.28 Gate.power :heat,:ON,limit to 5000mA @08:00:31.97 Mounted lysis Cartridge board#Q05061 @08:00:32.04 Using this cartridge's heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @08:09:19.19 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2085 => ["cartridge",:end,:insert,:inserted,:locked]} @08:09:27.94 -> PP.status.position 0 @08:09:36.19 -> Handoff.blocked @08:09:36.21 Handoff.select :blocked Handoff Valve::Manifold selects blocked @08:09:38.65 -> Syringe.psi 0.212826106024352 @08:10:13.25 -> PP.jog 1000 @08:10:13.31 PinPort.in :default @08:10:13.39 PinPort.jog 1000 Handoff Pin Port (PP) at washStation + 923 counts @08:10:26.08 -> PP.jog 500 @08:10:26.15 PinPort.jog 500 Handoff Pin Port (PP) at cartridge - 585 counts @08:10:51.82 -> PP.status.position 1500 @08:11:01.07 -> PP.jog 100; PP.status.position @08:11:01.13 PinPort.jog 100 1600 @08:11:12.30 -> PP.jog 100; PP.status.position @08:11:12.34 PinPort.jog 100 1700 @08:11:25.23 -> PP.jog 100; PP.status.position @08:11:25.27 PinPort.jog 100 1800 @08:11:26.16 -> PP.jog 100; PP.status.position @08:11:26.21 PinPort.jog 100 1900 @08:11:45.71 -> PP.jog 100; PP.status.position @08:11:45.74 PinPort.jog 100 2000 @08:12:08.59 -> Handoff.waste @08:12:08.63 Handoff.select :waste Handoff Valve::Manifold selects waste @08:12:35.75 -> Lysis1.push 100 @08:12:35.87 Lysis1.in :default @08:12:35.96 Lysis1.seek 1488uL Lysis Syringe #1 (LyS1) at 1488uL @08:12:50.47 -> delay 3; Lysis1.push 200 @08:12:50.50 delay 3 seconds @08:12:53.61 Lysis1.seek 1288uL Lysis Syringe #1 (LyS1) at 1288uL @08:13:15.42 -> PP Handoff Pin Port (PP) at cartridge - 85 counts @08:13:22.50 -> PP.status.position 2000 @08:13:29.60 -> Syringe.psi 0.208096637001574 @08:13:42.99 -> delay 3; Lysis1.push 200 @08:13:43.02 delay 3 seconds @08:13:46.16 Lysis1.seek 1088uL Lysis Syringe #1 (LyS1) at 1088uL @08:14:41.61 -> PP.clear @08:14:41.66 PinPort.in :unlocking @08:14:41.74 PinPort.seek :clear Handoff Pin Port (PP) clear @08:16:27.83 -> PP.jog 2000 @08:16:27.86 PinPort.in :default @08:16:27.96 PinPort.jog 2000 Handoff Pin Port (PP) at cartridge - 85 counts @08:16:41.45 -> PP.jog 50 @08:16:41.50 PinPort.jog 50 Handoff Pin Port (PP) at cartridge - 35 counts @08:16:45.20 -> Syringe.psi 0.174990353842247 @08:17:10.47 -> delay 3; Lysis1.push 200 @08:17:10.50 delay 3 seconds @08:17:13.62 Lysis1.seek 888uL Lysis Syringe #1 (LyS1) at 888uL @08:17:42.29 -> Handoff Handoff Valve::Manifold selects waste @08:18:01.58 -> delay 3; Lysis1.push 200 @08:18:01.61 delay 3 seconds @08:18:04.73 Lysis1.seek 688uL Lysis Syringe #1 (LyS1) at 688uL @08:18:21.80 -> PP.jog 50 @08:18:21.83 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 15 counts @08:18:40.93 -> delay 2; PP.jog 50 @08:18:40.96 delay 2 seconds @08:18:43.01 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 65 counts @08:18:59.27 -> delay 2; PP.jog 50 @08:18:59.30 delay 2 seconds @08:19:01.35 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 115 counts @08:19:23.84 -> delay 2; PP.jog 50 @08:19:23.88 delay 2 seconds @08:19:25.93 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 165 counts @08:20:16.47 -> PP.status.position 2250 @08:20:31.03 -> delay 2; PP.jog 50 @08:20:31.06 delay 2 seconds @08:20:33.11 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 215 counts @08:20:39.75 -> delay 3; Lysis1.push 200 @08:20:39.78 delay 3 seconds @08:20:42.89 Lysis1.seek 488uL Lysis Syringe #1 (LyS1) at 488uL @08:21:01.02 -> delay 2; PP.jog 50 @08:21:01.05 delay 2 seconds @08:21:03.10 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 265 counts @08:21:07.11 -> delay 3; Lysis1.push 200 @08:21:07.16 delay 3 seconds @08:21:10.25 Lysis1.seek 288uL Lysis Syringe #1 (LyS1) at 288uL @08:21:24.15 -> Lysis1.empty @08:21:24.18 Lysis1.seek :empty Lysis Syringe #1 (LyS1) empty @08:21:33.97 -> Lysis2.empty @08:21:34.02 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @08:22:14.89 -> PP.status.position 2350 @08:22:29.61 -> exit @08:22:29.64 Session Ended @08:23:22.23

Session Begun @08:23:25.33 -> MainThread.exit # @08:23:25.52 Handoff.select :close @08:23:25.76

Session Ended @10:53:14.04PDT18-Jul-24

Environmental Sample Processor built 01-Jul-24 (3743e013) @10:53:15.75 >BREAK< ESP3 Gateway v1.43 for core bus @10:53:15.78 >BREAK< ESP3 Gateway v1.43 for cartridge bus @10:53:19.30 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @10:53:19.97 Sea pressure (Abs) tare is 6% of full scale @10:53:24.57 Polling Can every 2:00 @10:53:24.62 Waiting for espclients... @10:53:25.36 Session Begun @10:53:35.94 -> ESP.ready! @10:53:36.02 Gate.power :core,:OFF @10:53:36.04 Gate.power :sampler,:OFF,limit to 3500mA @10:53:36.08 Gate.power :skimmer,:OFF,limit to 3500mA @10:53:39.85 Gate.power :main,:ON,limit to 5000mA @10:53:39.87 Gate.power :analytic1,:ON,limit to 4000mA @10:53:40.06 Powering core bus @10:53:42.84 Plunge.configure :default @10:53:42.95 SamplePump.configure :default @10:53:43.07 Toroid.configure :default @10:53:43.18 Carousel.configure :default @10:53:43.29 Abs.configure :default @10:53:43.39 PhaseSeparator.configure :default @10:53:43.50 Archive.configure :default @10:53:43.61 Air.configure :default @10:53:43.70 Lysis1.configure :default @10:53:44.05 Lysis2.configure :default @10:53:44.21 PinPort.configure :default @10:53:44.30 All elf controllers running firmware version 4.92 @10:53:44.35 Gate.power :motor,:ON,limit to 5000mA @10:53:47.24 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:53:47.27 AnalyticModule::Unknown in preston -- SPR @10:53:47.48 Plunge.home.jog 0 @10:53:48.29 PinPort.home.jog 0 @10:53:48.32 Lysis2.home.jog 0 @10:53:48.37 Air.home.jog 0 @10:53:48.41 Archive.home.jog 0 @10:53:48.48 Carousel.home.jog 0 @10:53:48.51 Toroid.home.jog 0 @10:53:48.57 Lysis1.home.jog 0 @10:53:49.36 Plunge.in :homing @10:53:49.71 Plunge.jog 1592 @10:53:49.78 Lysis2.in :homing @10:53:49.84 Air.in :homing @10:53:49.87 Toroid.in :homing @10:53:49.93 PinPort.in :homing @10:53:49.97 Lysis1.in :homing @10:53:50.01 Archive.in :homing @10:53:50.39 Lysis2.jog 1406 @10:53:50.44 Air.jog 2106 @10:53:50.48 Toroid.jog 600 @10:53:50.54 PinPort.jog -105 @10:53:51.01 Archive.jog 1400 @10:53:51.14 Lysis1.jog 2128 @10:53:51.57 PinPort.setRawPosition! 0 @10:53:51.59 Plunge.setRawPosition! 50 @10:53:51.75 Plunge.seek :home @10:53:51.78 PinPort.seek :home @10:53:51.99 PinPort.in :default @10:53:52.13 Plunge.in :default @10:53:52.32 Lysis2.setRawPosition! 50 @10:53:52.41 Lysis2.seek :home @10:53:52.55 Archive.setRawPosition! 50 @10:53:52.63 Archive.seek :home @10:53:52.79 Lysis2.in :default @10:53:52.80 Air.setRawPosition! 50 @10:53:52.99 Air.seek :home @10:53:53.12 Archive.in :default @10:53:53.14 Lysis1.setRawPosition! 50 @10:53:53.31 Lysis1.seek :home @10:53:53.45 Toroid.setRawPosition! 0 @10:53:53.47 Air.in :default @10:53:53.69 Toroid.seek :home @10:53:53.86 Toroid.in :default @10:53:53.89 Lysis1.in :default @10:53:54.31 Toroid.seek :clear @10:53:56.00 Carousel.in :default @10:53:56.18 Carousel.home.seek 25 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 25 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:54:02.95 -> to 25 @10:54:03.25 Carousel.seek 25 @10:54:03.52 Lysis1.in :fast @10:54:03.59 Lysis1.seek :locked @10:54:06.28 Plunge.seek :locked @10:54:06.50 Air.in :fast @10:54:06.59 Air.seek :locked @10:54:07.60 Carousel.reconfigure :locked @10:54:07.78 Powering cartridge bus @10:54:10.47 Gate.setIR 1750,5000,0,6 @10:54:10.60 Gate.setIR 0,5000,0,6 @10:54:10.70 PuckHeater.configure :default @10:54:10.79 Gate.power :heat,:ON,limit to 5000mA @10:54:11.56 Mounted lysis Cartridge board#Q05075 @10:54:11.62 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @10:54:11.78 -> Cartridge.sampler.source=ni @10:54:11.82 GenericSampler::InvalidExternalPump in preston -- :ni is not :sampler, :skimmer or nil @10:54:13.80 -> Cartridge.sampler.source=nil @10:54:32.50 -> PhytoSampler.primeVolume=PhytoSampler.bypassVolume=10 10 @10:54:35.83 -> slots {[45,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 25 => { Type: :lyseHold_phyto, State: :dry}} @10:54:51.12 -> fire lyseHold_phyto # @10:54:52.09 Toroid.seek :clear @10:54:52.33 Carousel.in :default @10:54:52.48 Carousel.home.seek 25 @10:54:52.90 Carousel.reconfigure :locked @10:54:53.14 Gate.setIR 1750,5000,0,6 @10:54:53.27 Gate.setIR 0,5000,0,6 @10:54:53.38 PuckHeater.configure :default @10:54:53.48 Gate.power :heat,:ON,limit to 5000mA @10:54:56.20 Mounted lysis Cartridge board#Q05075 @10:54:56.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:54:56.32 Selecting Cartridge 25 @10:54:56.78 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 25" @10:54:57.11 Preparing to filter cartridge 25 @10:54:57.46 Air.in :default @10:54:57.68 Priming sample loop w/10ml, bypass w/10ml @10:54:57.71 Air.seek :sealed @10:54:57.79 SamplePump.setPosition! 0ml @10:54:57.99 Bypass.open @10:54:59.59 Intake.open @10:54:59.73 upsync @10:55:00.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:55:03.02 Vacuum tare is 53% of full scale at 21C (drifted 1%) @10:55:03.20 Abs.in :default @10:55:03.28 Abs.hold @10:55:04.15 SamplePump.in :start @10:55:04.26 SamplePump.seek 10ml,1:10 @10:55:04.82 SamplePump.configure :prime @10:55:16.81 Abs.stop @10:55:16.91 SamplePump.setPosition! 0ml @10:55:16.99 Exhaust.open @10:55:18.34 Bypass.close @10:55:19.88 Abs.hold @10:55:20.28 SamplePump.in :start @10:55:20.39 SamplePump.seek 10ml,1:10 @10:55:20.97 SamplePump.configure :prime @10:55:32.97 Abs.stop @10:55:33.08 Bypass.open @10:55:33.77 Intake.close @10:55:34.58 Toroid.in :hold @10:55:34.66 Toroid.seek :crack @10:55:36.24 Toroid.seek :cartridge @10:55:37.38 Toroid.in :default @10:55:37.47 Toroid.seek :clear @10:55:39.54 Pressurizing cartridge to 26psi above 15.8psia @10:55:39.56 SamplePump.setPosition! 0ml @10:55:39.67 Intake.open @10:55:40.35 Bypass.close @10:55:41.03 Exhaust.close @10:55:41.80 Abs.in :default @10:55:41.89 Abs.hold @10:55:42.18 SamplePump.in :charge @10:55:42.28 SamplePump.seek 2.3ml,42 seconds @10:55:50.43 Abs.stop @10:55:50.52 Intake.close @10:55:51.27 Pressurized cartridge with 0.292ml to 26.5psi @10:55:51.29 Isolated @36.4psia (20.7psi) @10:55:51.33 Verifying cartridge holds at least 0.65..0.85 of 36.4psia @10:55:51.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:55:56.02 Cartridge pressure fell 2% to 35.9psia @10:55:56.19 Sampling 50.0ml @10:55:56.21 SamplePump.setPosition! 0ml @10:55:56.30 Exhaust.open @10:55:56.98 Intake.open @10:55:57.85 Abs.in :default @10:55:57.95 Abs.hold @10:55:58.88 SamplePump.in :start @10:55:58.99 SamplePump.seek 50ml,4:40 @10:55:59.57 SamplePump.configure :sample @10:56:01.98 Cartridge 25 filtering 2/50ml at 0.43ml/s with 15.6psi vacuum 5.4m deep (22.7psia) @10:57:02.03 Cartridge 25 filtering 37/50ml at 0.59ml/s with 10.5psi vacuum 11.7m deep (32.0psia) @10:57:24.78 Abs.stop @10:57:24.96 Intake.close @10:57:25.06 Exhaust.close @10:57:25.31 Toroid.in :hold @10:57:25.41 Toroid.seek :bypass @10:57:27.08 Toroid.in :default @10:57:27.17 Toroid.seek :clear @10:57:28.80 Sampled 50.0ml @10:57:29.28 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 25" @10:57:29.80 Handoff.select :waste @10:57:30.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:57:32.20 upsync @10:57:36.66 Syringe tare is 52% of full scale at 21C (drifted 1%) @10:57:36.70 PinPort.in :locking @10:57:36.80 PinPort.seek :locked @10:57:37.33 Archive.in :fast @10:57:37.40 Lysis2.in :fast @10:57:37.50 Lysis1.seek 1350uL @10:57:37.61 Archive.seek :full @10:57:37.74 Lysis2.seek :empty @10:57:45.87 Plunge.seek :reagent @10:57:46.81 Evacuating puck with filter bubble point of 24psi @10:57:46.85 PinPort.seek :locked @10:57:46.98 Handoff.select :waste @10:57:47.02 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:57:53.68 Air syringe pumping up to 1 strokes to reach 4psi @10:57:53.69 Air stroke #1 of 1 @10:57:53.83 Air.in :pressurize @10:57:53.92 Air.seek :pressurized @10:57:59.58 2.2psi after pressurize @10:57:59.61 Air.in :compress @10:57:59.72 Air.seek :evac @10:58:02.76 2.3psi after compress @10:58:13.35 0.6psi after compress (stable) @10:58:13.37 Air.in :default @10:58:13.47 Air.hold @10:58:13.71 at 0.4psi Opened to waste @10:58:13.73 Handoff.select :blocked @10:58:13.92 Archive syringe pumping up to 8 strokes to reach 24psi @10:58:13.93 Archive stroke #1 of 8 @10:58:14.08 Archive.in :pressurize @10:58:14.20 Archive.seek :pressurized @10:58:26.67 1.7psi after pressurize @10:58:32.26 1.2psi after pressurize (stable) @10:58:32.29 Archive.in :compress @10:58:32.40 Archive.seek :empty @10:58:48.18 17.6psi after compress @10:58:58.77 17.1psi after compress (stable) @10:58:58.80 Archive.in :gulp @10:58:58.92 Archive.seek :topVent @10:59:05.26 18.3psi after gulp @10:59:10.85 17.8psi after gulp (stable) @10:59:10.86 Archive stroke #2 of 8 @10:59:11.00 Archive.in :pressurize @10:59:11.09 Archive.seek :pressurized @10:59:23.61 18.2psi after pressurize @10:59:29.20 17.4psi after pressurize (stable) @10:59:29.23 Archive.in :compress @10:59:29.36 Archive.seek :empty @10:59:35.80 Reached 24.2 of 24.0psi after pumping 3261uL (1.88 strokes) @10:59:35.83 Archive.in :default @10:59:36.05 Archive.hold @10:59:36.20 Handoff.select :waste @10:59:36.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:59:49.95 delay 1:00 @11:00:49.98 Handoff.select :blocked @11:00:50.24 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:01:12.83 Handoff.select :waste @11:01:13.00 0.5psi at evacEnd @11:01:13.01 Handoff.select :blocked @11:01:13.20 Archive.in :fast @11:01:13.28 Archive.seek :topVent @11:01:19.78 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @11:01:26.37 Stable @0.1psi after Handoff blocked @11:01:26.51 Archive syringe pumping up to 8 strokes to reach 24psi @11:01:26.55 Archive stroke #1 of 8 @11:01:26.70 Archive.in :pressurize @11:01:26.79 Archive.seek :pressurized @11:01:39.25 5.0psi after pressurize @11:01:39.27 Archive.in :compress @11:01:39.49 Archive.seek :empty @11:01:55.24 15.8psi after compress @11:02:05.83 14.9psi after compress (stable) @11:02:05.86 Archive.in :gulp @11:02:05.99 Archive.seek :topVent @11:02:12.32 16.0psi after gulp @11:02:17.91 15.4psi after gulp (stable) @11:02:17.92 Archive stroke #2 of 8 @11:02:18.07 Archive.in :pressurize @11:02:18.19 Archive.seek :pressurized @11:02:30.65 15.7psi after pressurize @11:02:30.68 Archive.in :compress @11:02:30.80 Archive.seek :empty @11:02:38.99 Reached 24.2 of 24.1psi after pumping 3298uL (1.91 strokes) @11:02:39.01 Archive.in :default @11:02:39.57 Archive.hold @11:02:39.68 Handoff.select :waste @11:02:39.96 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:02:46.59 -> Cmd.stop @11:02:46.83 Handoff.select :close @11:02:46.98 Handoff.select :close @11:02:47.09 Gate.power :heat,:OFF @11:02:47.11 Gate.power :cartridge,:OFF @11:02:47.29 PinPort.in :unlocking @11:02:47.45 PinPort.seek :clear @11:02:47.48 PinPort.seek :clear @11:02:47.74 Slide::Error in preston -- Handoff Pin Port (PP) aborted at cartridge - 7 counts (sensed cartridge + 2 counts) @11:02:47.77 Retry #1 of 2 @11:02:47.82 PinPort.stop @11:02:48.07 Slide::Error in detached from preston -- Handoff Pin Port (PP) aborted at washStation + 552 counts (sensed washStation + 549 counts) @11:02:48.11 Retry #1 of 2 @11:02:48.31 Gate.power :analytic1,:OFF @11:02:48.34 Gate.power :motor,:OFF @11:02:48.39 Gate.power :core,:OFF @11:02:48.45 Gate.power :main,:OFF @11:02:48.52 Gate.power :sampler,:OFF,limit to 3500mA @11:02:48.54 Gate.power :skimmer,:OFF,limit to 3500mA @11:02:48.65 Gate.power :sampler,:OFF,limit to 3500mA @11:02:48.68 Gate.power :skimmer,:OFF,limit to 3500mA @11:02:52.25 Gate.power :main,:ON,limit to 5000mA @11:02:52.27 Gate.power :analytic1,:ON,limit to 4000mA @11:02:52.39 Powering core bus @11:02:52.44 Gate.power :analytic1,:ON,limit to 4000mA @11:02:54.64 Gate.power :core,:OFF @11:02:54.68 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2142 !! @11:02:54.79 Powering core bus @11:02:55.06 BusOff in detached from preston -- core bus was not powered while configuring HandoffValves @11:02:55.08 Retry #2 of 2 @11:02:55.19 Gate.power :analytic1,:OFF @11:02:55.21 Gate.power :core,:OFF @11:02:55.26 Gate.power :main,:OFF @11:02:55.34 Gate.power :sampler,:OFF,limit to 3500mA @11:02:55.37 Gate.power :skimmer,:OFF,limit to 3500mA @11:02:55.45 Powering core bus @11:02:58.32 Gate.power :motor,:ON,limit to 5000mA @11:02:58.37 AnalyticModule::Unknown in preston -- SPR @11:02:58.52 Slide::Error in preston -- Handoff Pin Port (PP) Not yet Homed! @11:02:58.55 Retry #2 of 2 @11:02:58.67 Gate.power :motor,:OFF @11:02:58.73 Gate.power :sampler,:OFF,limit to 3500mA @11:02:58.76 Gate.power :skimmer,:OFF,limit to 3500mA @11:02:59.06 Gate.power :main,:ON,limit to 5000mA @11:02:59.09 Gate.power :analytic1,:ON,limit to 4000mA @11:02:59.23 Gate.power :analytic1,:ON,limit to 4000mA @11:02:59.51 Plunge.configure :default @11:02:59.65 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:02:59.76 SamplePump.configure :default @11:02:59.95 Toroid.configure :default @11:03:00.17 Carousel.configure :locked @11:03:00.36 Abs.configure :default @11:03:00.55 PhaseSeparator.configure :default @11:03:00.74 Archive.configure :default @11:03:00.93 Air.configure :default @11:03:01.10 Lysis1.configure :default @11:03:01.56 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2142 !! @11:03:01.63 Gate.power :core,:OFF @11:03:01.71 Lysis2.configure :default @11:03:01.82 BusOff in preston -- Lysis Syringe #2 (LyS2) core bus was not powered @11:03:01.87 Powering core bus @11:03:01.97 Gate.power :analytic1,:OFF @11:03:02.01 Gate.power :core,:OFF @11:03:02.03 Gate.power :main,:OFF @11:03:02.08 ready to power off :STOPPED @11:03:02.89 Powering core bus @11:03:06.39 Gate.power :motor,:ON,limit to 5000mA @11:03:06.43 AnalyticModule::Unknown in detached from preston -- SPR @11:03:06.53 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:03:06.65 Slide::Error in detached from preston -- Handoff Pin Port (PP) Not yet Homed! @11:03:06.75 Gate.power :motor,:OFF @11:03:06.77 ready to power off @11:03:19.81 -> Cartridge.activate @11:03:19.96 Slide::Error in preston -- Storage Carousel (SC) Not yet Homed! @11:03:27.01 -> ESP.ready! @11:03:27.12 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @11:03:27.24 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @11:03:27.28 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @11:03:27.35 Slide::Error in preston -- Handoff Pin Port (PP) Not yet Homed! @11:03:29.69 upsync @11:03:44.65 -> ESP.reready! @11:03:44.79 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @11:03:44.85 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @11:03:44.92 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @11:03:44.99 Slide::Error in preston -- Handoff Pin Port (PP) Not yet Homed! @11:03:47.19 upsync @11:04:15.79 -> MainThread.exit # @11:04:15.99 Thread::Aborted in clearToroid -- "Aborted by preston" @11:04:16.01 Session Ended @11:04:16.03 Thread::Aborted in clearToroid -- "Aborted by preston" @11:04:16.12 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @11:04:16.15 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @11:04:16.18 ThreadError in clearToroid -- killed thread @11:04:16.20 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @11:04:25.48PDT18-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @11:04:27.17 >BREAK< ESP3 Gateway v1.43 for core bus @11:04:27.19 >BREAK< ESP3 Gateway v1.43 for cartridge bus @11:04:30.74 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @11:04:31.40 Sea pressure (Abs) tare is 6% of full scale @11:04:36.79 Polling Can every 2:00 @11:04:36.84 Waiting for espclients... @11:04:37.95 Session Begun @11:05:21.73 -> ESP.ready! @11:05:21.80 Gate.power :core,:OFF @11:05:21.83 Gate.power :sampler,:OFF,limit to 3500mA @11:05:21.87 Gate.power :skimmer,:OFF,limit to 3500mA @11:05:25.63 Gate.power :main,:ON,limit to 5000mA @11:05:25.65 Gate.power :analytic1,:ON,limit to 4000mA @11:05:25.85 Powering core bus @11:05:28.65 Plunge.configure :default @11:05:28.78 SamplePump.configure :default @11:05:28.91 Toroid.configure :default @11:05:29.02 Carousel.configure :default @11:05:29.13 Abs.configure :default @11:05:29.24 PhaseSeparator.configure :default @11:05:29.36 Archive.configure :default @11:05:29.48 Air.configure :default @11:05:29.83 Lysis1.configure :default @11:05:29.92 Lysis2.configure :default @11:05:30.03 PinPort.configure :default @11:05:30.13 All elf controllers running firmware version 4.92 @11:05:30.19 Gate.power :motor,:ON,limit to 5000mA @11:05:33.03 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:05:33.06 AnalyticModule::Unknown in preston -- SPR @11:05:33.51 Plunge.home.jog 0 @11:05:33.66 Toroid.home.jog 0 @11:05:33.77 Carousel.home.jog 0 @11:05:34.30 PinPort.home.jog 0 @11:05:34.39 Lysis2.home.jog 0 @11:05:34.42 Air.home.jog 0 @11:05:34.50 Lysis1.home.jog 0 @11:05:34.59 Archive.home.jog 0 @11:05:35.24 Toroid.in :homing @11:05:35.37 Plunge.in :homing @11:05:35.49 Toroid.jog 603 @11:05:35.57 Plunge.jog 2332 @11:05:35.81 PinPort.in :homing @11:05:35.87 Lysis2.in :homing @11:05:35.91 Air.in :homing @11:05:35.98 Archive.in :homing @11:05:36.07 Lysis1.in :homing @11:05:36.26 PinPort.jog -105 @11:05:36.33 Lysis2.jog 9841 @11:05:36.40 Air.jog 9003 @11:05:36.96 Archive.jog 9039 @11:05:37.02 Lysis1.jog 3318 @11:05:37.40 PinPort.setRawPosition! 0 @11:05:37.42 Plunge.setRawPosition! 50 @11:05:37.57 PinPort.seek :home @11:05:37.60 Plunge.seek :home @11:05:37.86 Toroid.setRawPosition! 0 @11:05:37.89 PinPort.in :default @11:05:38.03 Toroid.seek :home @11:05:38.22 Plunge.in :default @11:05:38.25 Toroid.in :default @11:05:39.30 Lysis1.setRawPosition! 50 @11:05:39.39 Lysis1.seek :home @11:05:39.71 Lysis1.in :default @11:05:41.92 Air.setRawPosition! 50 @11:05:42.05 Air.seek :home @11:05:42.28 Archive.setRawPosition! 50 @11:05:42.31 Lysis2.setRawPosition! 50 @11:05:42.46 Lysis2.seek :home @11:05:42.48 Archive.seek :home @11:05:42.68 Air.in :default @11:05:42.83 Lysis2.in :default @11:05:42.94 Archive.in :default @11:05:43.31 Toroid.seek :clear @11:05:44.98 Carousel.in :default @11:05:45.13 Carousel.home.seek 25 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 25 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:06:36.65 -> Cmd.stop @11:06:36.85 Carousel.in :default @11:06:37.03 Carousel.home.seek 25 @11:06:37.40 Lysis1.in :fast @11:06:37.50 Lysis1.seek :locked @11:06:40.05 Plunge.seek :locked @11:06:41.45 Lysis1.in :unlocking @11:06:41.61 Lysis1.seek :clear @11:06:43.14 Gate.power :analytic1,:OFF @11:06:43.18 Gate.power :motor,:OFF @11:06:43.20 Gate.power :core,:OFF @11:06:43.24 Gate.power :main,:OFF @11:06:43.28 Safely stopped and ready to power off :STOPPED @11:40:22.21 -> PhytoSampler.bypassVolume=PhytoSampler.primeVoume=10 @11:40:22.25 NoMethodError in preston -- undefined method `primeVoume=' for PhytoSampler:Cartridge::Sampler @11:40:30.78 -> PhytoSampler.bypassVolume=PhytoSampler.primeVolume=10 10 @11:40:47.75 -> ESP.ready! @11:40:47.82 Gate.power :sampler,:OFF,limit to 3500mA @11:40:47.86 Gate.power :skimmer,:OFF,limit to 3500mA @11:40:47.90 Gate.power :main,:ON,limit to 5000mA @11:40:47.94 Gate.power :analytic1,:ON,limit to 4000mA @11:40:48.05 Powering core bus @11:40:51.00 Plunge.configure :default @11:40:51.11 SamplePump.configure :default @11:40:51.23 Toroid.configure :default @11:40:51.34 Carousel.configure :default @11:40:51.44 Abs.configure :default @11:40:51.56 PhaseSeparator.configure :default @11:40:51.67 Archive.configure :default @11:40:51.79 Air.configure :default @11:40:51.88 Lysis1.configure :default @11:40:52.23 Lysis2.configure :default @11:40:52.35 PinPort.configure :default @11:40:52.45 All elf controllers running firmware version 4.92 @11:40:52.48 Gate.power :motor,:ON,limit to 5000mA @11:40:55.42 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:40:55.45 AnalyticModule::Unknown in preston -- SPR @11:40:55.78 Plunge.home.jog 0 @11:40:56.52 Lysis2.home.jog 0 @11:40:56.56 Air.home.jog 0 @11:40:56.62 Carousel.home.jog 0 @11:40:56.66 Lysis1.home.jog 0 @11:40:56.70 Archive.home.jog 0 @11:40:56.78 Toroid.home.jog 0 @11:40:56.83 PinPort.home.jog 0 @11:40:57.82 Plunge.in :homing @11:40:57.93 Plunge.jog 1597 @11:40:58.16 Lysis2.in :homing @11:40:58.26 Lysis1.in :homing @11:40:58.30 Toroid.in :homing @11:40:58.35 PinPort.in :homing @11:40:58.42 Air.in :homing @11:40:58.44 Archive.in :homing @11:40:58.71 Lysis2.jog 1406 @11:40:58.77 Lysis1.jog 1396 @11:40:58.84 PinPort.jog -110 @11:40:58.88 Toroid.jog 593 @11:40:58.94 Archive.jog 1400 @11:40:59.02 Air.jog 1369 @11:40:59.52 Plunge.setRawPosition! 50 @11:40:59.55 PinPort.setRawPosition! 0 @11:40:59.72 PinPort.seek :home @11:40:59.74 Plunge.seek :home @11:40:59.98 PinPort.in :default @11:41:00.16 Plunge.in :default @11:41:00.26 Lysis2.setRawPosition! 50 @11:41:00.35 Lysis2.seek :home @11:41:00.52 Lysis1.setRawPosition! 50 @11:41:00.54 Archive.setRawPosition! 50 @11:41:00.68 Lysis1.seek :home @11:41:00.73 Archive.seek :home @11:41:00.98 Air.setRawPosition! 50 @11:41:01.01 Lysis2.in :default @11:41:01.15 Air.seek :home @11:41:01.34 Lysis1.in :default @11:41:01.38 Archive.in :default @11:41:01.41 Toroid.setRawPosition! 0 @11:41:01.61 Toroid.seek :home @11:41:01.75 Air.in :default @11:41:01.88 Toroid.in :default @11:41:02.16 Toroid.seek :clear @11:41:03.90 Carousel.in :default @11:41:04.05 Carousel.home.seek 25 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 25 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.17psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:42:29.29 -> slots {[45,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 25 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50}} @11:42:48.31 -> slots 25=>[:dry,:lyseHold_phyto @11:42:48.34 SyntaxError in preston -- compile error preston:7: syntax error, unexpected $end, expecting ']' slots 25=>[:dry,:lyseHold_phyto ^ @11:42:50.72 -> slots 25=>[:dry,:lyseHold_phyto] {25 => { Type: :lyseHold_phyto, State: :dry}} @11:42:53.79 -> slots {[45,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 25 => { Type: :lyseHold_phyto, State: :dry}} @11:43:01.91 -> to 25 @11:43:02.29 Carousel.seek 25 @11:43:02.59 Lysis1.in :fast @11:43:02.68 Lysis1.seek :locked @11:43:05.35 Plunge.seek :locked @11:43:05.57 Air.in :fast @11:43:05.65 Air.seek :locked @11:43:06.65 Carousel.reconfigure :locked @11:43:06.83 Powering cartridge bus @11:43:09.50 Gate.setIR 1750,5000,0,6 @11:43:09.64 Gate.setIR 0,5000,0,6 @11:43:09.74 PuckHeater.configure :default @11:43:09.84 Gate.power :heat,:ON,limit to 5000mA @11:43:10.58 Mounted lysis Cartridge board#Q05075 @11:43:10.63 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @11:43:10.79 -> Cartridge.sampler.source=nil @11:43:26.50 -> fire lyseHold_phyto # @11:43:27.46 Toroid.seek :clear @11:43:27.68 Carousel.in :default @11:43:27.83 Carousel.home.seek 25 @11:43:28.27 Carousel.reconfigure :locked @11:43:28.52 Gate.setIR 1750,5000,0,6 @11:43:28.67 Gate.setIR 0,5000,0,6 @11:43:28.77 PuckHeater.configure :default @11:43:28.87 Gate.power :heat,:ON,limit to 5000mA @11:43:29.56 Mounted lysis Cartridge board#Q05075 @11:43:29.61 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:43:29.72 Selecting Cartridge 25 @11:43:30.18 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 25" @11:43:30.60 Preparing to filter cartridge 25 @11:43:31.47 Air.in :default @11:43:31.61 Air.seek :sealed @11:43:31.89 Priming sample loop w/10ml, bypass w/10ml @11:43:31.92 SamplePump.setPosition! 0ml @11:43:32.04 Bypass.open @11:43:33.57 upsync @11:43:33.70 Intake.open @11:43:34.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:43:37.13 Vacuum tare is 53% of full scale at 21C (drifted 1%) @11:43:37.32 Abs.in :default @11:43:37.41 Abs.hold @11:43:37.70 SamplePump.in :start @11:43:37.81 SamplePump.seek 10ml,1:10 @11:43:38.39 SamplePump.configure :prime @11:43:50.38 Abs.stop @11:43:50.47 SamplePump.setPosition! 0ml @11:43:50.56 Exhaust.open @11:43:51.95 Bypass.close @11:43:53.49 Abs.in :default @11:43:53.59 Abs.hold @11:43:53.93 SamplePump.in :start @11:43:54.04 SamplePump.seek 10ml,1:10 @11:43:54.62 SamplePump.configure :prime @11:44:06.66 Abs.stop @11:44:06.78 Bypass.open @11:44:07.49 Intake.close @11:44:08.28 Toroid.in :hold @11:44:08.38 Toroid.seek :crack @11:44:09.95 Toroid.seek :cartridge @11:44:11.10 Toroid.in :default @11:44:11.19 Toroid.seek :clear @11:44:13.27 Pressurizing cartridge to 26psi above 15.8psia @11:44:13.29 SamplePump.setPosition! 0ml @11:44:13.39 Intake.open @11:44:14.05 Bypass.close @11:44:14.76 Exhaust.close @11:44:16.03 Abs.in :default @11:44:16.14 Abs.hold @11:44:16.51 SamplePump.in :charge @11:44:16.60 SamplePump.seek 2.3ml,42 seconds @11:44:19.61 Abs.stop @11:44:19.72 Intake.close @11:44:20.47 Pressurized cartridge with 0.038ml to 26.1psi @11:44:20.49 Isolated @32.4psia (16.7psi) @11:44:20.54 Verifying cartridge holds at least 0.65..0.85 of 32.4psia @11:44:20.61 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:44:25.73 Cartridge pressure fell 21% to 25.6psia @11:44:25.90 Toroid.in :hold @11:44:26.00 Toroid.seek :bypass @11:44:27.68 Toroid.in :default @11:44:27.79 Toroid.seek :clear @11:44:29.55 Cartridge::Sampler::Leaky in FILTERING -- Retained only 25.6psia @11:44:29.58 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @11:44:29.63 BadNews.email "Retained only 25.6psia",Subject: "Cartridge::Sampler::Leaky in FILTERING" @11:44:31.80 upsync @11:44:56.90 -> Cartridge.unlock @11:44:56.97 Gate.power :heat,:OFF @11:44:57.02 Gate.power :cartridge,:OFF @11:44:57.13 Carousel.in :default @11:44:57.28 Carousel.home.seek 25 @11:44:57.74 Air.in :unlocking @11:44:57.91 Air.seek :clear @11:44:59.38 Lysis1.in :unlocking @11:44:59.56 Lysis1.seek :clear @11:44:59.67 Plunge.in :unlocking @11:44:59.80 Plunge.seek :clear @11:45:20.48 -> Cartridge.lock @11:45:20.68 Lysis1.in :fast @11:45:20.77 Lysis1.seek :locked @11:45:23.43 Plunge.in :default @11:45:23.53 Plunge.seek :locked @11:45:23.73 Air.in :fast @11:45:23.82 Air.seek :locked @11:45:24.82 Carousel.reconfigure :locked @11:45:25.48 Powering cartridge bus @11:45:28.17 Gate.setIR 1750,5000,0,6 @11:45:28.31 Gate.setIR 0,5000,0,6 @11:45:28.50 PuckHeater.configure :default @11:45:28.58 Gate.power :heat,:ON,limit to 5000mA @11:45:29.27 Mounted lysis Cartridge board#Q05075 @11:45:29.32 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @11:45:41.35 -> Thread[:FILTERING].resume # @11:45:41.41 Resuming Thread (suspended since 11:44:20) @11:45:41.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:45:41.81 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 49% to 16.4psia @11:45:41.83 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @11:45:41.88 BadNews.email "Isolated cartridge pressure fell by 49% to 16.4psia",Subject: "Cartridge::Sampler::Leaky in FILTERING" @11:45:44.16 upsync @11:46:02.64 -> Cmd.stop @11:46:02.77 SamplePump.stop @11:46:02.89 Gate.power :heat,:OFF @11:46:02.92 Gate.power :cartridge,:OFF @11:46:03.06 Carousel.in :default @11:46:03.21 Carousel.home.seek 25 @11:46:03.60 Plunge.seek :locked @11:46:03.89 Air.in :unlocking @11:46:04.06 Air.seek :clear @11:46:04.16 Lysis1.in :unlocking @11:46:04.37 Lysis1.seek :clear @11:46:05.91 Gate.power :analytic1,:OFF @11:46:05.93 Gate.power :motor,:OFF @11:46:05.98 Gate.power :core,:OFF @11:46:06.02 Gate.power :main,:OFF @11:46:06.05 Safely stopped and ready to power off :STOPPED @11:46:08.33 -> slots {[45,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 25 => { Type: :lyseHold_phyto, State: :leaky}} @11:46:17.11 -> slots 25=>[:dry,:lyseHold_phyto] {25 => { Type: :lyseHold_phyto, State: :dry}} @11:46:23.55 -> fire lyseHold_phyto # @11:46:23.76 Gate.power :sampler,:OFF,limit to 3500mA @11:46:23.82 Gate.power :skimmer,:OFF,limit to 3500mA @11:46:23.88 Gate.power :main,:ON,limit to 5000mA @11:46:23.92 Gate.power :analytic1,:ON,limit to 4000mA @11:46:24.05 Powering core bus @11:46:26.85 Plunge.configure :default @11:46:26.97 SamplePump.configure :default @11:46:27.08 Toroid.configure :default @11:46:27.20 Carousel.configure :default @11:46:27.30 Abs.configure :default @11:46:27.41 PhaseSeparator.configure :default @11:46:27.52 Archive.configure :default @11:46:27.64 Air.configure :default @11:46:27.73 Lysis1.configure :default @11:46:28.07 Lysis2.configure :default @11:46:28.22 PinPort.configure :default @11:46:28.31 All elf controllers running firmware version 4.92 @11:46:28.34 Gate.power :motor,:ON,limit to 5000mA @11:46:31.31 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:46:31.34 AnalyticModule::Unknown in detached from preston#2 -- SPR @11:46:31.71 Plunge.home.jog 0 @11:46:32.44 PinPort.home.jog 0 @11:46:32.47 Lysis1.home.jog 0 @11:46:33.02 Carousel.home.jog 0 @11:46:33.10 Air.home.jog 0 @11:46:33.15 Archive.home.jog 0 @11:46:33.19 Lysis2.home.jog 0 @11:46:33.24 Toroid.home.jog 0 @11:46:34.34 Plunge.in :homing @11:46:34.59 Plunge.jog 1594 @11:46:34.67 Lysis2.in :homing @11:46:34.72 Air.in :homing @11:46:34.78 Toroid.in :homing @11:46:34.84 PinPort.in :homing @11:46:34.89 Lysis1.in :homing @11:46:34.94 Archive.in :homing @11:46:35.31 Lysis2.jog 1404 @11:46:35.40 Air.jog 1375 @11:46:35.44 Toroid.jog 594 @11:46:35.50 PinPort.jog -106 @11:46:35.58 Lysis1.jog 1396 @11:46:35.62 Archive.jog 1402 @11:46:36.17 PinPort.setRawPosition! 0 @11:46:36.26 PinPort.seek :home @11:46:36.42 Plunge.setRawPosition! 50 @11:46:36.44 PinPort.in :default @11:46:36.58 Plunge.seek :home @11:46:36.95 Lysis2.setRawPosition! 50 @11:46:36.99 Air.setRawPosition! 50 @11:46:37.03 Plunge.in :default @11:46:37.26 Air.seek :home @11:46:37.29 Lysis2.seek :home @11:46:37.53 Lysis1.setRawPosition! 50 @11:46:37.55 Archive.setRawPosition! 50 @11:46:37.69 Lysis1.seek :home @11:46:37.76 Archive.seek :home @11:46:38.01 Air.in :default @11:46:38.04 Toroid.setRawPosition! 0 @11:46:38.09 Lysis2.in :default @11:46:38.32 Toroid.seek :home @11:46:38.50 Archive.in :default @11:46:38.55 Lysis1.in :default @11:46:38.59 Toroid.in :default @11:46:39.06 Toroid.seek :clear @11:46:40.81 Carousel.in :default @11:46:40.99 Carousel.home.seek 25 @11:46:41.28 Selecting Cartridge 25 @11:46:41.60 Carousel.seek 25 @11:46:41.88 Lysis1.in :fast @11:46:41.98 Lysis1.seek :locked @11:46:44.64 Plunge.seek :locked @11:46:44.84 Air.in :fast @11:46:44.92 Air.seek :locked @11:46:45.95 Carousel.reconfigure :locked @11:46:46.17 Powering cartridge bus @11:46:48.84 Gate.setIR 1750,5000,0,6 @11:46:48.97 Gate.setIR 0,5000,0,6 @11:46:49.07 PuckHeater.configure :default @11:46:49.64 Gate.power :heat,:ON,limit to 5000mA @11:46:50.45 Mounted lysis Cartridge board#Q05075 @11:46:50.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:46:50.56 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 25" @11:46:51.06 Preparing to filter cartridge 25 @11:46:51.45 Air.in :default @11:46:51.61 Air.seek :sealed @11:46:51.69 Bypass.open @11:46:52.81 Toroid.in :hold @11:46:53.01 Toroid.seek :crack @11:46:53.53 upsync @11:46:54.67 Toroid.seek :cartridge @11:46:55.82 Toroid.in :default @11:46:55.92 Toroid.seek :clear @11:46:57.75 Exhaust.open @11:46:58.70 Pressurizing cartridge to 26psi above 15.6psia @11:46:58.72 SamplePump.setPosition! 0ml @11:46:58.82 Intake.open @11:46:59.50 Bypass.close @11:47:00.16 Exhaust.close @11:47:00.94 Abs.in :default @11:47:01.03 Abs.hold @11:47:01.29 SamplePump.in :charge @11:47:01.39 SamplePump.seek 2.3ml,42 seconds @11:47:15.35 Abs.stop @11:47:15.46 Intake.close @11:47:16.23 Pressurized cartridge with 0.872ml to 26.4psi @11:47:16.25 Isolated @37.0psia (21.4psi) @11:47:16.30 Verifying cartridge holds at least 0.65..0.85 of 37.0psia @11:47:16.37 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:47:20.99 Cartridge pressure fell 1% to 36.8psia @11:47:21.14 Sampling 50.0ml @11:47:21.16 SamplePump.setPosition! 0ml @11:47:21.26 Exhaust.open @11:47:21.93 Intake.open @11:47:23.36 Abs.in :default @11:47:23.45 Abs.hold @11:47:23.89 SamplePump.in :start @11:47:23.99 SamplePump.seek 50ml,4:40 @11:47:24.59 SamplePump.configure :sample @11:47:26.99 Cartridge 25 filtering 2/50ml at 0.70ml/s with 23.3psi vacuum @11:48:27.05 Cartridge 25 filtering 37/50ml at 0.58ml/s with 29.9psi vacuum @11:48:49.65 Abs.stop @11:48:49.84 Intake.close @11:48:49.93 Exhaust.close @11:48:50.22 Toroid.in :hold @11:48:50.32 Toroid.seek :bypass @11:48:51.97 Toroid.in :default @11:48:52.06 Toroid.seek :clear @11:48:53.77 Sampled 50.0ml @11:48:54.26 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 25" @11:48:54.88 Handoff.select :waste @11:48:55.11 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:48:56.77 upsync @11:49:01.76 Syringe tare is 52% of full scale at 21C (drifted 1%) @11:49:01.80 PinPort.in :locking @11:49:01.92 PinPort.seek :locked @11:49:02.43 Archive.in :fast @11:49:02.49 Lysis2.in :fast @11:49:02.64 Lysis1.seek 1350uL @11:49:02.77 Archive.seek :full @11:49:02.84 Lysis2.seek :empty @11:49:10.99 Plunge.seek :reagent @11:49:11.92 Evacuating puck with filter bubble point of 24psi @11:49:12.49 PinPort.seek :locked @11:49:12.64 Handoff.select :waste @11:49:12.78 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:49:19.39 Air syringe pumping up to 1 strokes to reach 4psi @11:49:19.40 Air stroke #1 of 1 @11:49:19.55 Air.in :pressurize @11:49:19.64 Air.seek :pressurized @11:49:25.30 1.6psi after pressurize @11:49:25.33 Air.in :compress @11:49:25.45 Air.seek :evac @11:49:28.50 2.5psi after compress @11:49:39.09 0.7psi after compress (stable) @11:49:39.11 Air.in :default @11:49:39.21 Air.hold @11:49:39.46 at 0.5psi Opened to waste @11:49:39.48 Handoff.select :blocked @11:49:39.67 Archive syringe pumping up to 8 strokes to reach 24psi @11:49:39.68 Archive stroke #1 of 8 @11:49:39.82 Archive.in :pressurize @11:49:39.92 Archive.seek :pressurized @11:49:52.39 1.4psi after pressurize @11:50:12.49 8.7psi after pressurize (stable) @11:50:12.52 Archive.in :compress @11:50:12.62 Archive.seek :empty @11:50:28.36 17.6psi after compress @11:50:38.96 16.7psi after compress (stable) @11:50:38.99 Archive.in :gulp @11:50:39.09 Archive.seek :topVent @11:50:45.40 18.1psi after gulp @11:50:50.99 17.3psi after gulp (stable) @11:50:51.01 Archive stroke #2 of 8 @11:50:51.15 Archive.in :pressurize @11:50:51.74 Archive.seek :pressurized @11:51:04.22 17.6psi after pressurize @11:51:09.81 17.0psi after pressurize (stable) @11:51:09.84 Archive.in :compress @11:51:09.95 Archive.seek :empty @11:51:16.16 Reached 23.9 of 24.0psi after pumping 3253uL (1.88 strokes) @11:51:16.18 Archive.in :default @11:51:16.30 Archive.hold @11:51:16.41 Handoff.select :waste @11:51:16.58 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:51:29.18 delay 1:00 @11:52:29.21 Handoff.select :blocked @11:52:29.43 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:52:52.02 Handoff.select :waste @11:52:52.28 0.6psi at evacEnd @11:52:52.29 Handoff.select :blocked @11:52:52.48 Archive.in :fast @11:52:52.58 Archive.seek :topVent @11:52:59.07 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @11:53:05.66 Stable @0.3psi after Handoff blocked @11:53:05.70 Archive syringe pumping up to 8 strokes to reach 24psi @11:53:05.74 Archive stroke #1 of 8 @11:53:05.86 Archive.in :pressurize @11:53:05.95 Archive.seek :pressurized @11:53:18.41 4.7psi after pressurize @11:53:18.44 Archive.in :compress @11:53:18.56 Archive.seek :empty @11:53:34.29 15.5psi after compress @11:53:44.89 14.7psi after compress (stable) @11:53:45.02 Archive.in :gulp @11:53:45.14 Archive.seek :topVent @11:53:51.49 16.0psi after gulp @11:53:57.09 15.2psi after gulp (stable) @11:53:57.10 Archive stroke #2 of 8 @11:53:57.26 Archive.in :pressurize @11:53:57.35 Archive.seek :pressurized @11:54:09.80 15.6psi after pressurize @11:54:09.83 Archive.in :compress @11:54:09.95 Archive.seek :empty @11:54:18.81 Reached 24.5 of 24.3psi after pumping 3312uL (1.91 strokes) @11:54:18.83 Archive.in :default @11:54:18.92 Archive.hold @11:54:19.04 Handoff.select :waste @11:54:19.22 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:54:26.31 delay 1:00 @11:55:26.34 Handoff.select :blocked @11:55:26.52 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:55:49.11 Handoff.select :waste @11:55:49.31 0.5psi at evacEnd @11:55:49.34 Air.seek :fullVent @11:55:50.91 Archive.in :fast @11:55:51.01 Archive.seek :topVent @11:55:57.70 Seawater evacuated, Pressure above/below filter released. @11:55:57.83 Lysis1.in :slow @11:55:57.91 Lysis1.seek 1050uL @11:56:27.58 delay 5 seconds @11:56:32.61 Handoff.select :blocked @11:56:32.84 Lysis1.seek 450uL @11:57:32.01 delay 3 seconds @11:57:35.11 Air.in mix with 1.26318 parts Lysis1 @11:57:35.96 Air.seek :evac @11:57:36.00 Lysis1.seek 300uL @11:57:51.03 Lysis1.seek :empty @11:58:20.75 Lysis buffer delivered @11.4psi @11:58:20.77 Polling Can every 2:00 @11:58:21.35 PuckHeater.configure :default #ambient 21.3C @11:58:21.45 PuckHeater.seek 79.8C @11:59:16.34 PuckHeater.hold 2:00,79.8C @12:01:15.84 Polling Can every 2:00 @12:01:16.39 PuckHeater.configure :default #ambient 21.3C @12:01:16.49 PuckHeater.hold 30 seconds,78.7C @12:01:46.56 Polling Can every 2:00 @12:01:47.11 PuckHeater.configure :default #ambient 21.2C @12:01:47.20 PuckHeater.hold 30 seconds,78.2C @12:02:17.29 Polling Can every 2:00 @12:02:17.94 PuckHeater.configure :default #ambient 21.3C @12:02:18.04 PuckHeater.hold 10:00,77.7C @12:12:15.04 Lysis complete. Syringe pressure @16.0psi @12:12:15.07 Archive syringe pumping up to 5 strokes to reach 40psi @12:12:15.11 Archive stroke #1 of 5 @12:12:15.26 Archive.in :pressurize @12:12:15.35 Archive.seek :pressurized @12:12:27.81 16.0psi after pressurize @12:12:33.90 15.2psi after pressurize (stable) @12:12:33.93 Archive.in :compress @12:12:34.06 Archive.seek :empty @12:12:42.83 Reached 40.3 of 40.0psi after pumping 1579uL (0.91 strokes) @12:12:42.86 Archive.in :default @12:12:42.96 Archive.hold @12:12:43.08 delay 2 seconds @12:12:45.11 Archive.in :fast @12:12:45.20 Archive.seek :full @12:12:51.94 Lysis1.seek 16uL @12:12:53.64 delay 1 second @12:12:54.68 Archive.seek :full @12:12:54.79 Lysis1.seek :empty @12:12:56.54 Handoff.select :blocked @12:12:56.74 Initial air pressure @41.4psi @12:12:56.86 Lysis1.in :extract @12:12:57.00 Lysis1.seek 600uL,nil @12:13:01.27 Archive syringe pumping up to 6 strokes to reach 25psi @12:13:01.29 Archive stroke #1 of 6 @12:13:01.49 Archive.in :pressurize @12:13:01.59 Archive.seek :pressurized @12:13:02.66 Archive.in :default @12:13:02.84 Archive.hold @12:13:03.20 Initial 600uL lysate @13.7psi @12:13:03.22 Handoff.select :blocked @12:13:03.37 Initial air pressure @13.1psi @12:13:03.49 Lysis1.seek 825uL,nil @12:13:03.63 Archive syringe pumping up to 6 strokes to reach 17psi @12:13:03.65 Archive stroke #1 of 6 @12:13:03.87 Archive.in :pressurize @12:13:03.98 Archive.seek :pressurized @12:13:15.34 13.8psi after pressurize @12:13:16.85 Reached 17.0 of 17.0psi after pumping 1397uL (0.81 strokes) @12:13:16.88 Archive.in :default @12:13:16.98 Archive.hold @12:13:18.39 Archive syringe pumping up to 6 strokes to reach 17psi @12:13:18.41 Archive stroke #1 of 6 @12:13:18.57 Archive.in :compress @12:13:18.70 Archive.seek :empty @12:13:19.67 Archive.in :default @12:13:19.75 Archive.hold @12:13:20.08 Recovered 825uL lysate @16.2psi @12:13:20.15 Air.in :halfSpeed @12:13:20.27 Air.seek :fullVent @12:13:22.73 Puck Top Vented, bottom @6.2psi @12:13:22.75 Handoff.select :waste @12:13:22.94 delay 5 seconds @12:13:28.02 Handoff open to waste, bottom @0.2psi @12:13:28.05 Air.in :default @12:13:28.18 Air.seek :evac @12:13:29.93 Handoff.select :close @12:13:30.15 Gate.power :heat,:OFF @12:13:30.18 Gate.power :cartridge,:OFF @12:13:30.30 PinPort.in :unlocking @12:13:30.40 PinPort.seek :clear @12:13:30.92 Carousel.in :default @12:13:31.08 Carousel.home.seek 25 @12:13:31.45 Plunge.seek :locked @12:13:32.38 Archive.in :unlocking @12:13:32.56 Archive.seek :clear @12:13:32.62 Air.in :unlocking @12:13:32.88 Air.seek :clear @12:13:32.99 Lysis1.in :unlocking @12:13:33.21 Lysis1.seek :clear @12:13:33.26 Lysis2.in :unlocking @12:13:33.44 Lysis2.seek :clear @12:13:38.11 Gate.power :analytic1,:OFF @12:13:38.15 Gate.power :motor,:OFF @12:13:38.30 Gate.power :core,:OFF @12:13:38.34 Gate.power :main,:OFF @12:13:38.39 Safely stopped and ready to power off @12:14:00.11 -> ESP.ready! @12:14:00.19 Gate.power :sampler,:OFF,limit to 3500mA @12:14:00.25 Gate.power :skimmer,:OFF,limit to 3500mA @12:14:00.28 Gate.power :main,:ON,limit to 5000mA @12:14:00.33 Gate.power :analytic1,:ON,limit to 4000mA @12:14:00.44 Powering core bus @12:14:03.22 Plunge.configure :default @12:14:03.35 SamplePump.configure :default @12:14:03.46 Toroid.configure :default @12:14:03.56 Carousel.configure :default @12:14:03.66 Abs.configure :default @12:14:03.78 PhaseSeparator.configure :default @12:14:03.89 Archive.configure :default @12:14:04.01 Air.configure :default @12:14:04.10 Lysis1.configure :default @12:14:04.49 Lysis2.configure :default @12:14:04.60 PinPort.configure :default @12:14:04.69 All elf controllers running firmware version 4.92 @12:14:04.72 Gate.power :motor,:ON,limit to 5000mA @12:14:07.70 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:14:07.72 AnalyticModule::Unknown in preston -- SPR @12:14:08.15 Plunge.home.jog 0 @12:14:09.30 Lysis2.home.jog 0 @12:14:09.41 Air.home.jog 0 @12:14:09.52 Carousel.home.jog 0 @12:14:09.60 PinPort.home.jog 0 @12:14:09.64 Lysis1.home.jog 0 @12:14:09.67 Toroid.home.jog 0 @12:14:09.78 Archive.home.jog 0 @12:14:10.51 Plunge.in :homing @12:14:10.93 Plunge.jog 1609 @12:14:11.22 Lysis2.in :homing @12:14:11.25 Lysis1.in :homing @12:14:11.30 Archive.in :homing @12:14:11.36 PinPort.in :homing @12:14:11.40 Air.in :homing @12:14:11.45 Toroid.in :homing @12:14:11.74 Lysis2.jog 1407 @12:14:11.79 Lysis1.jog 1395 @12:14:11.84 PinPort.jog -108 @12:14:11.92 Archive.jog 1404 @12:14:11.98 Air.jog 1376 @12:14:12.02 Toroid.jog 593 @12:14:12.56 Plunge.setRawPosition! 50 @12:14:12.59 PinPort.setRawPosition! 0 @12:14:12.76 PinPort.seek :home @12:14:12.79 Plunge.seek :home @12:14:13.04 PinPort.in :default @12:14:13.18 Plunge.in :default @12:14:13.30 Lysis2.setRawPosition! 50 @12:14:13.38 Lysis2.seek :home @12:14:13.54 Lysis1.setRawPosition! 50 @12:14:13.56 Air.setRawPosition! 50 @12:14:13.60 Archive.setRawPosition! 50 @12:14:13.83 Lysis1.seek :home @12:14:13.86 Air.seek :home @12:14:13.91 Archive.seek :home @12:14:14.31 Lysis2.in :default @12:14:14.35 Lysis1.in :default @12:14:14.57 Air.in :default @12:14:14.59 Archive.in :default @12:14:14.64 Toroid.setRawPosition! 0 @12:14:14.82 Toroid.seek :home @12:14:14.94 Toroid.in :default @12:14:15.11 Toroid.seek :clear @12:14:16.84 Carousel.in :default @12:14:17.00 Carousel.home.seek 25 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 25 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:14:39.59 -> slots {[45,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 25 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}} @12:15:03.02 -> SC.to 37 @12:15:03.07 Carousel.seek 37 Storage Carousel (SC) at 37 @12:25:30.83 -> #with biomass @12:25:41.34 -> slots 37=>[:dry,:lyseHold_phyto] {37 => { Type: :lyseHold_phyto, State: :dry}} @12:25:53.78 -> fire lyseHold_phyto # @12:25:54.81 Toroid.seek :clear @12:25:55.02 Carousel.in :default @12:25:55.18 Carousel.home.seek 37 @12:25:55.47 Selecting Cartridge 37 @12:25:55.80 Carousel.seek 37 @12:25:56.08 Lysis1.in :fast @12:25:56.21 Lysis1.seek :locked @12:25:58.89 Plunge.seek :locked @12:25:59.21 Air.in :fast @12:25:59.30 Air.seek :locked @12:26:00.31 Carousel.reconfigure :locked @12:26:00.50 Powering cartridge bus @12:26:03.17 Gate.setIR 1750,5000,0,6 @12:26:03.31 Gate.setIR 0,5000,0,6 @12:26:03.41 PuckHeater.configure :default #ambient 21.3C @12:26:03.51 Gate.power :heat,:ON,limit to 5000mA @12:26:04.25 Mounted lysis Cartridge board#Q05092 @12:26:04.31 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:26:04.37 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 37" @12:26:04.79 Preparing to filter cartridge 37 @12:26:05.17 Air.in :default @12:26:05.33 Air.seek :sealed @12:26:05.59 Priming sample loop w/10ml, bypass w/10ml @12:26:05.61 SamplePump.setPosition! 0ml @12:26:05.79 Bypass.open @12:26:08.08 upsync @12:26:08.18 Intake.open @12:26:08.96 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:26:11.59 Vacuum tare is 53% of full scale at 21C (drifted 1%) @12:26:11.77 Abs.in :default @12:26:11.85 Abs.hold @12:26:12.17 SamplePump.in :start @12:26:12.28 SamplePump.seek 10ml,1:10 @12:26:12.86 SamplePump.configure :prime @12:26:24.86 Abs.stop @12:26:24.95 SamplePump.setPosition! 0ml @12:26:25.04 Exhaust.open @12:26:26.46 Bypass.close @12:26:28.03 Abs.in :default @12:26:28.15 Abs.hold @12:26:28.48 SamplePump.in :start @12:26:28.59 SamplePump.seek 10ml,1:10 @12:26:29.17 SamplePump.configure :prime @12:26:41.18 Abs.stop @12:26:41.30 Bypass.open @12:26:42.02 Intake.close @12:26:42.80 Toroid.in :hold @12:26:42.89 Toroid.seek :crack @12:26:44.47 Toroid.seek :cartridge @12:26:45.61 Toroid.in :default @12:26:45.71 Toroid.seek :clear @12:26:47.78 Pressurizing cartridge to 26psi above 15.8psia @12:26:47.81 SamplePump.setPosition! 0ml @12:26:47.91 Intake.open @12:26:48.57 Bypass.close @12:26:49.29 Exhaust.close @12:26:50.12 Abs.in :default @12:26:50.22 Abs.hold @12:26:50.49 SamplePump.in :charge @12:26:50.58 SamplePump.seek 2.3ml,42 seconds @12:27:05.90 Abs.stop @12:27:06.60 Intake.close @12:27:07.37 Pressurized cartridge with 1.052ml to 26.1psi @12:27:07.39 Isolated @38.4psia (22.7psi) @12:27:07.41 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @12:27:07.50 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:27:12.14 Cartridge pressure fell 3% to 37.4psia @12:27:12.28 Sampling 50.0ml @12:27:12.30 SamplePump.setPosition! 0ml @12:27:12.42 Exhaust.open @12:27:13.11 Intake.open @12:27:13.99 Abs.in :default @12:27:14.07 Abs.hold @12:27:14.49 SamplePump.in :start @12:27:14.60 SamplePump.seek 50ml,4:40 @12:27:15.18 SamplePump.configure :sample @12:27:17.58 Cartridge 37 filtering 2/50ml at 0.72ml/s with 29.9psi vacuum @12:28:17.64 Cartridge 37 filtering 38/50ml at 0.59ml/s with 24.5psi vacuum @12:28:39.16 Abs.stop @12:28:39.34 Intake.close @12:28:39.46 Exhaust.close @12:28:39.70 Toroid.in :hold @12:28:39.79 Toroid.seek :bypass @12:28:41.46 Toroid.in :default @12:28:41.55 Toroid.seek :clear @12:28:43.18 Sampled 50.0ml @12:28:43.62 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 37" @12:28:44.25 Handoff.select :waste @12:28:44.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:28:48.09 upsync @12:28:51.14 Syringe tare is 52% of full scale at 21C (drifted 1%) @12:28:51.18 PinPort.in :locking @12:28:51.28 PinPort.seek :locked @12:28:51.81 Archive.in :fast @12:28:51.87 Lysis2.in :fast @12:28:51.97 Lysis1.seek 1350uL @12:28:52.19 Archive.seek :full @12:28:52.25 Lysis2.seek :empty @12:29:00.40 Plunge.seek :reagent @12:29:01.33 Evacuating puck with filter bubble point of 24psi @12:29:01.36 PinPort.seek :locked @12:29:01.52 Handoff.select :waste @12:29:01.56 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:29:08.20 Air syringe pumping up to 1 strokes to reach 4psi @12:29:08.21 Air stroke #1 of 1 @12:29:08.38 Air.in :pressurize @12:29:08.47 Air.seek :pressurized @12:29:14.17 3.0psi after pressurize @12:29:14.20 Air.in :compress @12:29:14.31 Air.seek :evac @12:29:17.36 3.7psi after compress @12:29:29.95 1.1psi after compress (stable) @12:29:30.06 Air.in :default @12:29:30.21 Air.hold @12:29:30.46 at 1.0psi Opened to waste @12:29:30.48 Handoff.select :blocked @12:29:30.66 Archive syringe pumping up to 8 strokes to reach 24psi @12:29:30.67 Archive stroke #1 of 8 @12:29:30.81 Archive.in :pressurize @12:29:30.90 Archive.seek :pressurized @12:29:43.36 2.3psi after pressurize @12:29:48.95 1.7psi after pressurize (stable) @12:29:48.98 Archive.in :compress @12:29:49.08 Archive.seek :empty @12:30:04.82 20.2psi after compress @12:30:15.41 20.1psi after compress (stable) @12:30:15.44 Archive.in :gulp @12:30:15.57 Archive.seek :topVent @12:30:21.92 21.4psi after gulp @12:30:27.51 20.8psi after gulp (stable) @12:30:27.62 Archive stroke #2 of 8 @12:30:27.78 Archive.in :pressurize @12:30:27.86 Archive.seek :pressurized @12:30:40.33 21.3psi after pressurize @12:30:45.92 20.6psi after pressurize (stable) @12:30:45.96 Archive.in :compress @12:30:46.07 Archive.seek :empty @12:30:53.02 Reached 24.2 of 24.0psi after pumping 3270uL (1.89 strokes) @12:30:53.04 Archive.in :default @12:30:53.15 Archive.hold @12:30:53.26 Handoff.select :waste @12:30:53.42 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:31:05.01 delay 1:00 @12:32:05.04 Handoff.select :blocked @12:32:05.20 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:32:27.79 Handoff.select :waste @12:32:28.07 0.7psi at evacEnd @12:32:28.09 Handoff.select :blocked @12:32:28.29 Archive.in :fast @12:32:28.37 Archive.seek :topVent @12:32:34.90 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @12:32:41.49 Stable @0.5psi after Handoff blocked @12:32:41.53 Archive syringe pumping up to 8 strokes to reach 25psi @12:32:41.56 Archive stroke #1 of 8 @12:32:41.70 Archive.in :pressurize @12:32:41.79 Archive.seek :pressurized @12:32:54.26 2.3psi after pressurize @12:32:54.29 Archive.in :compress @12:32:54.41 Archive.seek :empty @12:33:10.21 16.2psi after compress @12:33:20.80 15.5psi after compress (stable) @12:33:20.92 Archive.in :gulp @12:33:21.03 Archive.seek :topVent @12:33:27.37 16.4psi after gulp @12:33:32.97 15.8psi after gulp (stable) @12:33:32.99 Archive stroke #2 of 8 @12:33:33.13 Archive.in :pressurize @12:33:33.20 Archive.seek :pressurized @12:33:45.67 16.2psi after pressurize @12:33:45.70 Archive.in :compress @12:33:45.80 Archive.seek :empty @12:33:56.15 Reached 24.5 of 24.5psi after pumping 3344uL (1.93 strokes) @12:33:56.18 Archive.in :default @12:33:56.29 Archive.hold @12:33:56.40 Handoff.select :waste @12:33:56.57 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:34:03.67 delay 1:00 @12:35:03.71 Handoff.select :blocked @12:35:03.88 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:35:26.47 Handoff.select :waste @12:35:26.67 0.5psi at evacEnd @12:35:26.70 Air.seek :fullVent @12:35:28.29 Archive.in :fast @12:35:28.38 Archive.seek :topVent @12:35:35.17 Seawater evacuated, Pressure above/below filter released. @12:35:35.30 Lysis1.in :slow @12:35:35.40 Lysis1.seek 1050uL @12:36:05.07 delay 5 seconds @12:36:10.11 Handoff.select :blocked @12:36:10.36 Lysis1.seek 450uL @12:37:09.55 delay 3 seconds @12:37:12.65 Air.in mix with 1.26318 parts Lysis1 @12:37:13.54 Air.seek :evac @12:37:13.58 Lysis1.seek 300uL @12:37:28.59 Lysis1.seek :empty @12:37:58.31 Lysis buffer delivered @16.7psi @12:37:58.33 Polling Can every 2:00 @12:37:58.91 PuckHeater.configure :default #ambient 21.3C @12:37:58.99 PuckHeater.seek 79.8C @12:38:50.58 PuckHeater.hold 2:00,79.8C @12:40:50.49 Polling Can every 2:00 @12:40:51.04 PuckHeater.configure :default #ambient 21.2C @12:40:51.13 PuckHeater.hold 30 seconds,78.7C @12:41:21.31 Polling Can every 2:00 @12:41:21.86 PuckHeater.configure :default #ambient 21.2C @12:41:21.95 PuckHeater.hold 30 seconds,78.2C @12:41:52.16 Polling Can every 2:00 @12:41:52.71 PuckHeater.configure :default #ambient 21.2C @12:41:52.80 PuckHeater.hold 10:00,77.7C @12:51:51.85 Lysis complete. Syringe pressure @18.8psi @12:51:51.98 Archive syringe pumping up to 5 strokes to reach 40psi @12:51:52.00 Archive stroke #1 of 5 @12:51:52.16 Archive.in :pressurize @12:51:52.25 Archive.seek :pressurized @12:52:04.71 18.8psi after pressurize @12:52:10.80 18.3psi after pressurize (stable) @12:52:10.83 Archive.in :compress @12:52:10.94 Archive.seek :empty @12:52:18.67 Reached 40.3 of 40.0psi after pumping 1558uL (0.90 strokes) @12:52:18.69 Archive.in :default @12:52:18.80 Archive.hold @12:52:18.92 delay 2 seconds @12:52:20.97 Archive.in :fast @12:52:21.05 Archive.seek :full @12:52:27.71 Lysis1.seek 16uL @12:52:29.52 delay 1 second @12:52:30.56 Archive.seek :full @12:52:30.66 Lysis1.seek :empty @12:52:32.42 Handoff.select :blocked @12:52:32.62 Initial air pressure @41.4psi @12:52:32.74 Lysis1.in :extract @12:52:32.87 Lysis1.seek 600uL,nil @12:52:36.54 Archive syringe pumping up to 6 strokes to reach 25psi @12:52:36.55 Archive stroke #1 of 6 @12:52:36.71 Archive.in :pressurize @12:52:36.82 Archive.seek :pressurized @12:52:49.31 12.3psi after pressurize @12:52:55.18 Archive.in :default @12:52:55.25 Archive.hold @12:52:55.43 Initial 600uL lysate @14.4psi @12:52:55.45 Handoff.select :blocked @12:52:55.60 Initial air pressure @14.7psi @12:52:55.71 Lysis1.seek 825uL,nil @12:52:55.84 Archive syringe pumping up to 6 strokes to reach 17psi @12:52:55.86 Archive stroke #1 of 6 @12:52:56.08 Archive.in :compress @12:52:56.82 Archive.seek :empty @12:52:57.37 Reached 15.9 of 17.0psi after pumping 1398uL (0.81 strokes) @12:52:57.40 Archive.in :default @12:52:57.54 Archive.hold @12:52:57.86 Archive syringe pumping up to 6 strokes to reach 17psi @12:52:57.90 Archive stroke #1 of 6 @12:52:58.14 Archive.in :compress @12:52:58.31 Archive.seek :empty @12:53:05.17 Archive.in :default @12:53:05.24 Archive.hold @12:53:05.53 Recovered 825uL lysate @17.1psi @12:53:05.56 Air.in :halfSpeed @12:53:05.67 Air.seek :fullVent @12:53:08.15 Puck Top Vented, bottom @4.2psi @12:53:08.17 Handoff.select :waste @12:53:08.36 delay 5 seconds @12:53:13.45 Handoff open to waste, bottom @0.1psi @12:53:13.47 Air.in :default @12:53:13.58 Air.seek :evac @12:53:15.32 Handoff.select :close @12:53:15.51 Gate.power :heat,:OFF @12:53:15.54 Gate.power :cartridge,:OFF @12:53:15.68 PinPort.in :unlocking @12:53:15.76 PinPort.seek :clear @12:53:16.28 Carousel.in :default @12:53:16.44 Carousel.home.seek 37 @12:53:16.79 Plunge.seek :locked @12:53:17.72 Archive.in :unlocking @12:53:17.86 Archive.seek :clear @12:53:18.01 Air.in :unlocking @12:53:18.21 Air.seek :clear @12:53:18.27 Lysis1.in :unlocking @12:53:19.00 Lysis1.seek :clear @12:53:19.20 Lysis2.in :unlocking @12:53:19.34 Lysis2.seek :clear @12:53:24.00 Gate.power :analytic1,:OFF @12:53:24.02 Gate.power :motor,:OFF @12:53:24.06 Gate.power :core,:OFF @12:53:24.14 Gate.power :main,:OFF @12:53:24.17 Safely stopped and ready to power off @12:53:36.55 -> Cartridge.unlock @12:53:36.65 BusOff in preston -- core bus was not powered while reading Handoff Pin Port (PP)'s status @12:53:42.19 -> ESP.ready! @12:53:42.26 Gate.power :sampler,:OFF,limit to 3500mA @12:53:42.29 Gate.power :skimmer,:OFF,limit to 3500mA @12:53:42.33 Gate.power :main,:ON,limit to 5000mA @12:53:42.37 Gate.power :analytic1,:ON,limit to 4000mA @12:53:42.50 Powering core bus @12:53:45.29 Plunge.configure :default @12:53:45.42 SamplePump.configure :default @12:53:45.53 Toroid.configure :default @12:53:45.64 Carousel.configure :default @12:53:45.76 Abs.configure :default @12:53:45.87 PhaseSeparator.configure :default @12:53:45.97 Archive.configure :default @12:53:46.11 Air.configure :default @12:53:46.22 Lysis1.configure :default @12:53:46.58 Lysis2.configure :default @12:53:46.69 PinPort.configure :default @12:53:46.76 All elf controllers running firmware version 4.92 @12:53:46.79 Gate.power :motor,:ON,limit to 5000mA @12:53:49.76 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @12:53:49.79 AnalyticModule::Unknown in preston -- SPR @12:53:50.23 Plunge.home.jog 0 @12:53:50.81 PinPort.home.jog 0 @12:53:50.88 Lysis1.home.jog 0 @12:53:50.92 Archive.home.jog 0 @12:53:50.97 Toroid.home.jog 0 @12:53:51.03 Air.home.jog 0 @12:53:51.08 Carousel.home.jog 0 @12:53:51.15 Lysis2.home.jog 0 @12:53:52.07 Plunge.in :homing @12:53:52.39 Plunge.jog 1602 @12:53:52.62 Lysis2.in :homing @12:53:52.70 Lysis1.in :homing @12:53:52.77 Toroid.in :homing @12:53:52.82 PinPort.in :homing @12:53:52.87 Archive.in :homing @12:53:52.96 Air.in :homing @12:53:53.18 Lysis2.jog 1404 @12:53:53.26 Lysis1.jog 1395 @12:53:53.34 PinPort.jog -108 @12:53:53.45 Toroid.jog 601 @12:53:53.57 Air.jog 1374 @12:53:53.63 Archive.jog 1402 @12:53:54.07 PinPort.setRawPosition! 0 @12:53:54.08 Plunge.setRawPosition! 50 @12:53:54.26 Plunge.seek :home @12:53:54.32 PinPort.seek :home @12:53:54.55 PinPort.in :default @12:53:55.17 Plunge.in :default @12:53:55.44 Lysis2.setRawPosition! 50 @12:53:55.49 Lysis1.setRawPosition! 50 @12:53:55.54 Air.setRawPosition! 50 @12:53:55.59 Archive.setRawPosition! 50 @12:53:55.82 Lysis2.seek :home @12:53:55.89 Lysis1.seek :home @12:53:55.93 Air.seek :home @12:53:56.00 Archive.seek :home @12:53:56.41 Toroid.setRawPosition! 0 @12:53:56.51 Toroid.seek :home @12:53:56.74 Lysis1.in :default @12:53:56.78 Air.in :default @12:53:56.83 Lysis2.in :default @12:53:56.86 Archive.in :default @12:53:56.89 Toroid.in :default @12:53:57.46 Toroid.seek :clear @12:53:59.19 Carousel.in :default @12:53:59.34 Carousel.home.seek 37 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 37 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:55:02.97 -> Cartridge.lock @12:55:03.16 Lysis1.in :fast @12:55:03.24 Lysis1.seek :locked @12:55:05.90 Plunge.seek :locked @12:55:06.10 Air.in :fast @12:55:06.21 Air.seek :locked @12:55:07.22 Carousel.reconfigure :locked @12:55:07.41 Powering cartridge bus @12:55:10.08 Gate.setIR 1750,5000,0,6 @12:55:10.23 Gate.setIR 0,5000,0,6 @12:55:10.34 Gate.power :cartridge,:OFF @12:55:10.92 Mounted archive Cartridge @12:55:11.02 Cartridge::Incompatible in preston -- archive sensed when expecting lyseHold_phyto in slot 37 @12:55:25.82 -> to 36 @12:55:26.07 Carousel.in :default @12:55:26.27 Carousel.home.seek 37 @12:55:26.72 Air.in :unlocking @12:55:26.88 Air.seek :clear @12:55:27.91 Lysis1.in :unlocking @12:55:28.05 Lysis1.seek :clear @12:55:28.23 Plunge.in :unlocking @12:55:28.33 Plunge.seek :clear @12:55:30.00 Carousel.seek 36 @12:55:30.65 Lysis1.in :fast @12:55:30.75 Lysis1.seek :locked @12:55:33.44 Plunge.in :default @12:55:33.53 Plunge.seek :locked @12:55:33.75 Air.in :fast @12:55:33.83 Air.seek :locked @12:55:34.85 Carousel.reconfigure :locked @12:55:35.05 Powering cartridge bus @12:55:37.71 Gate.setIR 1750,5000,0,6 @12:55:37.84 Gate.setIR 0,5000,0,6 @12:55:37.94 PuckHeater.configure :default #ambient 21.2C @12:55:38.05 Gate.power :heat,:ON,limit to 5000mA @12:55:38.75 Mounted lysis Cartridge board#Q05075 @12:55:38.81 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @12:55:38.99 -> PhytoSampler.prepareLGcart 0,0 @12:55:39.02 Handoff.select :waste @12:55:39.20 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:55:45.83 Syringe tare is 52% of full scale at 21C (drifted 1%) @12:55:45.86 PinPort.in :locking @12:55:45.98 PinPort.seek :locked @12:55:46.51 Archive.in :fast @12:55:46.62 Lysis2.in :fast @12:55:46.68 Lysis1.seek :empty @12:55:46.77 Archive.seek :full @12:55:46.88 Lysis2.seek :empty Archive Syringe (ArS) full @12:56:03.82 -> Air.eva @12:56:03.86 NoMethodError in preston -- undefined method `eva' for Air:Syringe @12:56:05.52 -> Air.evac @12:56:05.57 Air.in :default @12:56:05.65 Air.seek :evac Air Syringe (AirS) at evac @12:56:48.46 -> delay 3; Air.fVent @12:56:48.48 delay 3 seconds @12:56:51.54 Air.seek :fVent Air Syringe (AirS) at fVent @12:57:21.03 -> delay 2; 3.times {Air.evac; Air.fVent} @12:57:21.06 delay 2 seconds @12:57:23.11 Air.seek :evac @12:57:24.67 Air.seek :fVent @12:57:26.23 Air.seek :evac @12:57:27.78 Air.seek :fVent @12:57:29.34 Air.seek :evac @12:57:30.90 Air.seek :fVent 3 @12:57:54.73 -> Air.legend {-11650 => "extended", -8285 => ["empty",0,:end], -8210 => [:fVent,:fullVent], -7700 => :evac, -7000 => "pressurized", -1715 => "sealed", -855 => ["full",1730,:topVent,:tVent,:cartridge,:locked,:lock], 0 => ["clear",:home,:begin], 50 => :limit} @12:58:13.45 -> Air.jog 50 @12:58:13.48 Air.jog 50 Air Syringe (AirS) at 29uL @13:00:45.06 -> AirS.evac @13:00:45.12 Air.seek :evac Air Syringe (AirS) at evac @13:00:52.53 -> AirS.jog -100 @13:00:52.56 Air.jog -100 Air Syringe (AirS) at 113uL @13:00:55.41 -> AirS.jog -100 @13:00:55.46 Air.jog -100 Air Syringe (AirS) at 90uL @13:00:57.87 -> AirS.jog -100 @13:00:57.90 Air.jog -100 Air Syringe (AirS) at 66uL @13:01:00.19 -> AirS.jog -100 @13:01:00.24 Air.jog -100 Air Syringe (AirS) at 43uL @13:01:06.86 -> Air.rawPosition -8100 @13:01:13.29 -> Air.legend {-11650 => "extended", -8285 => ["empty",0,:end], -8210 => [:fVent,:fullVent], -7700 => :evac, -7000 => "pressurized", -1715 => "sealed", -855 => ["full",1730,:topVent,:tVent,:cartridge,:locked,:lock], 0 => ["clear",:home,:begin], 50 => :limit} @13:02:31.23 -> exit @13:02:31.26 Session Ended @13:02:45.32 Session Begun @13:02:48.88 -> Cartridge.unlock @13:02:48.94 Gate.power :heat,:OFF @13:02:48.96 Gate.power :cartridge,:OFF @13:02:49.10 PinPort.in :unlocking @13:02:49.20 PinPort.seek :clear @13:02:49.69 Carousel.in :default @13:02:49.84 Carousel.home.seek 36 @13:02:50.26 Archive.in :unlocking @13:02:50.41 Archive.seek :clear @13:02:50.59 Air.in :unlocking @13:02:50.75 Air.seek :clear @13:02:50.88 Lysis2.in :unlocking @13:02:51.00 Lysis2.seek :clear @13:02:55.59 Lysis1.in :unlocking @13:02:55.75 Lysis1.seek :clear @13:02:55.88 Plunge.in :unlocking @13:02:55.96 Plunge.seek :clear @13:03:01.80 -> MainThread.exit # @13:03:01.97 Handoff.select :close @13:03:02.24 Session Ended @13:03:15.15PDT18-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:03:16.85 >BREAK< ESP3 Gateway v1.43 for core bus @13:03:16.88 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:03:20.40 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @13:03:21.08 Sea pressure (Abs) tare is 6% of full scale @13:03:25.84 Polling Can every 2:00 @13:03:25.89 Waiting for espclients... @13:03:26.80 Session Begun @13:03:28.15 -> ESP.ready! @13:03:28.35 Gate.power :sampler,:OFF,limit to 3500mA @13:03:28.37 Gate.power :skimmer,:OFF,limit to 3500mA @13:03:28.42 Gate.power :analytic1,:ON,limit to 4000mA @13:03:29.15 Plunge.configure :default @13:03:29.25 Plunge.hold @13:03:29.35 Plunge.configure :default @13:03:29.52 SamplePump.configure :default @13:03:29.70 Toroid.configure :default @13:03:29.79 Toroid.hold @13:03:29.89 Toroid.configure :default @13:03:30.06 Carousel.configure :default @13:03:30.19 Carousel.hold @13:03:30.30 Carousel.configure :default @13:03:30.74 Abs.configure :default @13:03:30.92 PhaseSeparator.configure :default @13:03:31.09 Archive.configure :default @13:03:31.18 Archive.hold @13:03:31.32 Archive.configure :default @13:03:31.49 Air.configure :default @13:03:31.60 Air.hold @13:03:31.70 Air.configure :default @13:03:31.85 Lysis1.configure :default @13:03:31.95 Lysis1.hold @13:03:32.06 Lysis1.configure :default @13:03:32.52 Lysis2.configure :default @13:03:32.61 Lysis2.hold @13:03:32.72 Lysis2.configure :default @13:03:32.90 PinPort.configure :default @13:03:32.99 PinPort.hold @13:03:33.10 PinPort.configure :default @13:03:33.20 All elf controllers running firmware version 4.92 @13:03:33.94 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:03:33.97 AnalyticModule::Unknown in preston -- SPR @13:03:35.20 Toroid.seek :clear @13:03:35.38 Carousel.in :default @13:03:35.53 Carousel.home.seek 36 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 36 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:11:21.60 -> slots {[45,25,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}} @13:11:42.12 -> SC.to 55 @13:11:42.22 Carousel.seek 55 Storage Carousel (SC) at 55 @13:12:11.48 -> Air.legend {-11650 => "extended", -8285 => ["empty",0,:end], -8110 => [:fVent,:fullVent], -7700 => :evac, -7000 => "pressurized", -1715 => "sealed", -855 => ["full",1730,:topVent,:tVent,:cartridge,:locked,:lock], 0 => ["clear",:home,:begin], 50 => :limit} @13:12:24.55 -> SC Storage Carousel (SC) at 55 @13:12:37.41 -> slot 55= [:dry,:lyseHold_phyto] @13:12:37.45 SyntaxError in preston -- compile error preston:6: syntax error, unexpected '=', expecting $end slot 55= [:dry,:lyseHold_phyto] ^ @13:12:44.81 -> slot 55=> [:dry,:lyseHold_phyto] {55 => { Type: :lyseHold_phyto, State: :dry}} @13:13:22.04 -> PhytoSampler.primeVolume=PhytoSampler.bypassVolume=10 10 @13:13:30.14 -> Cartridge.lock @13:13:30.34 Lysis1.in :fast @13:13:30.43 Lysis1.seek :locked @13:13:33.08 Plunge.seek :locked @13:13:33.27 Air.in :fast @13:13:33.37 Air.seek :locked @13:13:34.38 Carousel.reconfigure :locked @13:13:34.55 Powering cartridge bus @13:13:37.24 Gate.setIR 1750,5000,0,6 @13:13:37.38 Gate.setIR 0,5000,0,6 @13:13:37.48 PuckHeater.configure :default @13:13:37.58 Gate.power :heat,:ON,limit to 5000mA @13:13:40.36 Mounted lysis Cartridge board#Q05067 @13:13:40.42 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @13:13:43.84 -> Cartridge.sampler.source=nil @13:14:08.24 -> fire lyseHold_phyto # @13:14:09.21 Toroid.seek :clear @13:14:09.41 Carousel.in :default @13:14:09.54 Carousel.home.seek 55 @13:14:10.48 Carousel.reconfigure :locked @13:14:10.73 Gate.setIR 1750,5000,0,6 @13:14:10.86 Gate.setIR 0,5000,0,6 @13:14:10.96 PuckHeater.configure :default @13:14:11.06 Gate.power :heat,:ON,limit to 5000mA @13:14:13.74 Mounted lysis Cartridge board#Q05067 @13:14:13.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:14:13.86 Selecting Cartridge 55 @13:14:14.32 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 55" @13:14:14.72 Preparing to filter cartridge 55 @13:14:15.13 Air.in :default @13:14:15.34 Air.seek :sealed @13:14:15.52 Priming sample loop w/10ml, bypass w/10ml @13:14:15.55 SamplePump.setPosition! 0ml @13:14:15.72 Exhaust.close @13:14:15.90 Bypass.open @13:14:17.44 Intake.open @13:14:18.46 upsync @13:14:18.49 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:14:21.15 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:14:21.35 Abs.in :default @13:14:21.42 Abs.hold @13:14:21.71 SamplePump.in :start @13:14:21.82 SamplePump.seek 10ml,1:10 @13:14:22.40 SamplePump.configure :prime @13:14:34.38 Abs.stop @13:14:34.47 SamplePump.setPosition! 0ml @13:14:34.55 Exhaust.open @13:14:35.93 Bypass.close @13:14:37.46 Abs.in :default @13:14:37.56 Abs.hold @13:14:37.92 SamplePump.in :start @13:14:38.04 SamplePump.seek 10ml,1:10 @13:14:38.63 SamplePump.configure :prime @13:14:51.02 Abs.stop @13:14:51.13 Bypass.open @13:14:51.80 Intake.close @13:14:52.63 Toroid.in :hold @13:14:52.73 Toroid.seek :crack @13:14:54.31 Toroid.seek :cartridge @13:14:55.45 Toroid.in :default @13:14:55.56 Toroid.seek :clear @13:14:57.63 Pressurizing cartridge to 26psi above 15.8psia @13:14:57.65 SamplePump.setPosition! 0ml @13:14:57.75 Intake.open @13:14:58.47 Bypass.close @13:14:59.14 Exhaust.close @13:14:59.91 Abs.hold @13:15:00.23 SamplePump.in :charge @13:15:00.33 SamplePump.seek 2.3ml,42 seconds @13:15:15.47 Abs.stop @13:15:15.58 Intake.close @13:15:16.36 Pressurized cartridge with 1.027ml to 26.1psi @13:15:16.38 Isolated @39.0psia (23.2psi) @13:15:16.42 Verifying cartridge holds at least 0.65..0.85 of 39.0psia @13:15:16.49 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:15:21.11 Cartridge pressure fell 1% to 38.8psia @13:15:21.25 Sampling 50.0ml @13:15:21.27 SamplePump.setPosition! 0ml @13:15:21.37 Exhaust.open @13:15:22.02 Intake.open @13:15:22.89 Abs.in :default @13:15:22.98 Abs.hold @13:15:23.34 SamplePump.in :start @13:15:23.45 SamplePump.seek 50ml,4:40 @13:15:24.03 SamplePump.configure :sample @13:15:26.44 Cartridge 55 filtering 2/50ml at 0.59ml/s with 20.0psi vacuum @13:16:26.49 Cartridge 55 filtering 35/50ml at 0.56ml/s with 10.3psi vacuum 8.1m deep (26.6psia) @13:16:54.50 Abs.stop @13:16:54.69 Intake.close @13:16:54.74 Exhaust.close @13:16:55.03 Toroid.in :hold @13:16:55.12 Toroid.seek :bypass @13:16:57.38 Toroid.in :default @13:16:57.49 Toroid.seek :clear @13:16:59.14 Sampled 50.0ml @13:16:59.63 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 55" @13:17:00.25 Handoff.select :waste @13:17:00.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:17:02.16 upsync @13:17:07.12 Syringe tare is 52% of full scale at 21C (drifted 1%) @13:17:07.17 PinPort.in :locking @13:17:07.26 PinPort.seek :locked @13:17:07.80 Archive.in :fast @13:17:07.91 Lysis2.in :fast @13:17:07.97 Lysis1.seek 1350uL @13:17:08.09 Archive.seek :full @13:17:08.19 Lysis2.seek :empty @13:17:16.37 Plunge.seek :reagent @13:17:17.30 Evacuating puck with filter bubble point of 24psi @13:17:17.34 PinPort.seek :locked @13:17:17.48 Handoff.select :waste @13:17:17.52 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:17:24.17 Air syringe pumping up to 1 strokes to reach 4psi @13:17:24.21 Air stroke #1 of 1 @13:17:24.36 Air.in :pressurize @13:17:24.44 Air.seek :pressurized @13:17:30.10 0.9psi after pressurize @13:17:30.16 Air.in :compress @13:17:30.26 Air.seek :evac @13:17:33.77 1.8psi after compress @13:17:44.37 0.2psi after compress (stable) @13:17:44.39 Air.in :default @13:17:44.48 Air.hold @13:17:44.79 at 0.1psi Opened to waste @13:17:44.81 Handoff.select :blocked @13:17:45.00 Archive syringe pumping up to 8 strokes to reach 24psi @13:17:45.01 Archive stroke #1 of 8 @13:17:45.16 Archive.in :pressurize @13:17:45.25 Archive.seek :pressurized @13:17:57.71 10.0psi after pressurize @13:18:10.81 11.8psi after pressurize (stable) @13:18:10.84 Archive.in :compress @13:18:10.95 Archive.seek :empty @13:18:24.39 Reached 24.4 of 24.0psi after pumping 1681uL (0.97 strokes) @13:18:24.42 Archive.in :default @13:18:24.53 Archive.hold @13:18:24.64 Handoff.select :waste @13:18:24.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:18:43.90 delay 1:00 @13:19:43.93 Handoff.select :blocked @13:19:44.17 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @13:20:06.76 Handoff.select :waste @13:20:06.92 0.9psi at evacEnd @13:20:06.93 Handoff.select :blocked @13:20:07.12 Archive.in :fast @13:20:07.20 Archive.seek :topVent @13:20:14.24 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @13:20:20.83 Stable @0.8psi after Handoff blocked @13:20:20.87 Archive syringe pumping up to 8 strokes to reach 25psi @13:20:20.91 Archive stroke #1 of 8 @13:20:21.05 Archive.in :pressurize @13:20:21.13 Archive.seek :pressurized @13:20:33.58 1.4psi after pressurize @13:20:33.61 Archive.in :compress @13:20:33.74 Archive.seek :empty @13:20:49.48 16.5psi after compress @13:21:00.08 15.9psi after compress (stable) @13:21:00.13 Archive.in :gulp @13:21:00.25 Archive.seek :topVent @13:21:07.03 16.5psi after gulp @13:21:12.62 16.5psi after gulp (stable) @13:21:12.64 Archive stroke #2 of 8 @13:21:12.78 Archive.in :pressurize @13:21:12.88 Archive.seek :pressurized @13:21:25.34 16.8psi after pressurize @13:21:25.37 Archive.in :compress @13:21:25.46 Archive.seek :empty @13:21:35.79 Reached 24.7 of 24.8psi after pumping 3342uL (1.93 strokes) @13:21:35.82 Archive.in :default @13:21:35.92 Archive.hold @13:21:36.03 Handoff.select :waste @13:21:36.24 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:21:43.33 delay 1:00 @13:22:43.37 Handoff.select :blocked @13:22:43.53 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @13:23:06.15 Handoff.select :waste @13:23:06.30 0.4psi at evacEnd @13:23:06.32 Air.seek :fullVent @13:23:07.71 Archive.in :fast @13:23:07.81 Archive.seek :topVent @13:23:14.66 Seawater evacuated, Pressure above/below filter released. @13:23:14.79 Lysis1.in :slow @13:23:14.87 Lysis1.seek 1050uL @13:23:44.52 delay 5 seconds @13:23:49.54 Handoff.select :blocked @13:23:49.79 Lysis1.seek 450uL @13:24:48.92 delay 3 seconds @13:24:52.02 Air.in mix with 1.57127 parts Lysis1 @13:24:52.80 Air.seek :evac @13:24:52.87 Lysis1.seek 300uL @13:25:07.83 Lysis1.seek :empty @13:25:37.52 Lysis buffer delivered @28.0psi @13:25:37.55 Polling Can every 2:00 @13:25:38.15 PuckHeater.configure :default #ambient 21.3C @13:25:38.24 PuckHeater.seek 79.8C @13:26:30.86 PuckHeater.hold 2:00,79.8C @13:28:30.76 Polling Can every 2:00 @13:28:31.31 PuckHeater.hold 30 seconds,78.7C @13:29:01.50 Polling Can every 2:00 @13:29:02.05 PuckHeater.configure :default #ambient 21.3C @13:29:02.17 PuckHeater.hold 30 seconds,78.2C @13:29:32.35 Polling Can every 2:00 @13:29:32.91 PuckHeater.hold 10:00,77.7C @13:36:03.41 -> Syringe.psi 37.3982762975 @13:39:32.25 Lysis complete. Syringe pressure @37.4psi @13:39:32.39 Archive syringe pumping up to 5 strokes to reach 40psi @13:39:32.41 Archive stroke #1 of 5 @13:39:32.54 Archive.in :pressurize @13:39:32.62 Archive.seek :pressurized @13:39:45.06 36.9psi after pressurize @13:39:50.65 36.0psi after pressurize (stable) @13:39:50.69 Archive.in :compress @13:39:50.80 Archive.seek :empty @13:39:57.79 Reached 42.1 of 40.0psi after pumping 1543uL (0.89 strokes) @13:39:57.82 Archive.in :default @13:39:57.93 Archive.hold @13:39:58.03 delay 2 seconds @13:40:00.07 Archive.in :fast @13:40:00.19 Archive.seek :full @13:40:06.79 Lysis1.seek 16uL @13:40:08.49 delay 1 second @13:40:09.54 Archive.seek :full @13:40:09.64 Lysis1.seek :empty @13:40:11.42 Handoff.select :blocked @13:40:11.62 Initial air pressure @49.6psi @13:40:11.71 Lysis1.in :extract @13:40:11.89 Lysis1.seek 600uL,nil @13:40:14.35 Archive syringe pumping up to 6 strokes to reach 25psi @13:40:14.37 Archive stroke #1 of 6 @13:40:14.58 Archive.in :pressurize @13:40:14.67 Archive.seek :pressurized @13:40:27.13 15.3psi after pressurize @13:40:32.79 14.9psi after pressurize (stable) @13:40:32.81 Archive.in :compress @13:40:32.93 Archive.seek :empty @13:40:41.37 Archive.in :default @13:40:41.44 Archive.hold @13:40:41.73 Initial 600uL lysate @16.5psi @13:40:41.75 Handoff.select :blocked @13:40:41.91 Initial air pressure @17.8psi @13:40:42.04 Lysis1.seek 825uL,nil @13:40:42.84 Archive syringe pumping up to 6 strokes to reach 17psi @13:40:42.86 Archive stroke #1 of 6 @13:40:43.07 Archive.in :compress @13:40:43.17 Archive.seek :empty @13:40:46.79 Archive.in :default @13:40:46.88 Archive.hold @13:40:47.19 Recovered 825uL lysate @16.4psi @13:40:47.22 Air.in :halfSpeed @13:40:47.34 Air.seek :fullVent @13:40:49.38 Puck Top Vented, bottom @21.9psi @13:40:49.39 Handoff.select :waste @13:40:49.57 delay 5 seconds @13:40:54.65 Handoff open to waste, bottom @0.5psi @13:40:54.68 Air.in :default @13:40:54.80 Air.seek :evac @13:40:56.37 Handoff.select :close @13:40:56.56 Gate.power :heat,:OFF @13:40:56.59 Gate.power :cartridge,:OFF @13:40:56.72 PinPort.in :unlocking @13:40:56.80 PinPort.seek :clear @13:40:57.32 Carousel.in :default @13:40:57.47 Carousel.home.seek 55 @13:40:57.83 Plunge.seek :locked @13:40:58.76 Archive.in :unlocking @13:40:58.91 Archive.seek :clear @13:40:59.05 Air.in :unlocking @13:40:59.20 Air.seek :clear @13:40:59.35 Lysis1.in :unlocking @13:40:59.50 Lysis1.seek :clear @13:40:59.64 Lysis2.in :unlocking @13:40:59.74 Lysis2.seek :clear @13:41:04.37 Gate.power :analytic1,:OFF @13:41:04.40 Gate.power :motor,:OFF @13:41:04.44 Gate.power :core,:OFF @13:41:04.47 Gate.power :main,:OFF @13:41:04.51 Safely stopped and ready to power off @13:41:35.46 -> ESP.ready! @13:41:35.54 Gate.power :sampler,:OFF,limit to 3500mA @13:41:35.56 Gate.power :skimmer,:OFF,limit to 3500mA @13:41:35.60 Gate.power :main,:ON,limit to 5000mA @13:41:35.65 Gate.power :analytic1,:ON,limit to 4000mA @13:41:35.76 Powering core bus @13:41:39.12 Plunge.configure :default @13:41:39.25 SamplePump.configure :default @13:41:39.36 Toroid.configure :default @13:41:39.47 Carousel.configure :default @13:41:39.85 Abs.configure :default @13:41:39.97 PhaseSeparator.configure :default @13:41:40.08 Archive.configure :default @13:41:40.23 Air.configure :default @13:41:40.31 Lysis1.configure :default @13:41:40.39 Lysis2.configure :default @13:41:40.50 PinPort.configure :default @13:41:40.60 All elf controllers running firmware version 4.92 @13:41:40.64 Gate.power :motor,:ON,limit to 5000mA @13:41:43.03 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:41:43.06 AnalyticModule::Unknown in preston -- SPR @13:41:43.46 Plunge.home.jog 0 @13:41:43.64 Toroid.home.jog 0 @13:41:43.76 Carousel.home.jog 0 @13:41:44.33 Lysis1.home.jog 0 @13:41:44.42 Air.home.jog 0 @13:41:44.49 Lysis2.home.jog 0 @13:41:44.56 Archive.home.jog 0 @13:41:44.70 PinPort.home.jog 0 @13:41:45.37 Plunge.in :homing @13:41:45.48 Toroid.in :homing @13:41:45.70 Plunge.jog 1603 @13:41:45.73 Toroid.jog 620 @13:41:46.02 Lysis2.in :homing @13:41:46.07 Lysis1.in :homing @13:41:46.17 Archive.in :homing @13:41:46.22 PinPort.in :homing @13:41:46.26 Air.in :homing @13:41:46.49 Lysis2.jog 1401 @13:41:46.63 Lysis1.jog 1395 @13:41:46.73 PinPort.jog -107 @13:41:46.77 Archive.jog 1402 @13:41:46.82 Air.jog 1371 @13:41:47.19 PinPort.setRawPosition! 0 @13:41:47.25 Plunge.setRawPosition! 50 @13:41:47.31 PinPort.seek :home @13:41:47.46 Plunge.seek :home @13:41:47.63 PinPort.in :default @13:41:47.76 Lysis2.setRawPosition! 50 @13:41:47.84 Lysis2.seek :home @13:41:47.99 Toroid.setRawPosition! 0 @13:41:48.02 Plunge.in :default @13:41:48.24 Toroid.seek :home @13:41:48.41 Lysis1.setRawPosition! 50 @13:41:48.47 Air.setRawPosition! 50 @13:41:48.51 Lysis2.in :default @13:41:48.55 Archive.setRawPosition! 50 @13:41:48.59 Toroid.in :default @13:41:48.87 Lysis1.seek :home @13:41:48.90 Air.seek :home @13:41:48.94 Archive.seek :home @13:41:49.33 Lysis1.in :default @13:41:49.48 Air.in :default @13:41:49.50 Archive.in :default @13:41:49.93 Toroid.seek :clear @13:41:51.62 Carousel.in :default @13:41:51.79 Carousel.home.seek 55 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:42:05.41 -> slots {[45,25,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}} @13:42:58.27 -> SC.to 1 @13:42:58.32 Carousel.seek 1 Storage Carousel (SC) at 1 @13:43:41.66 -> slot 1=>[:dry,:lyseHold_phyto] {1 => { Type: :lyseHold_phyto, State: :dry}} @13:43:51.79 -> fire lyseHold_phyto # @13:43:52.79 Toroid.seek :clear @13:43:53.00 Carousel.in :default @13:43:53.15 Carousel.home.seek 1 @13:43:53.44 Selecting Cartridge 1 @13:43:53.77 Carousel.seek 1 @13:43:54.06 Lysis1.in :fast @13:43:54.19 Lysis1.seek :locked @13:43:56.85 Plunge.seek :locked @13:43:57.05 Air.in :fast @13:43:57.14 Air.seek :locked @13:43:58.16 Carousel.reconfigure :locked @13:43:58.34 Powering cartridge bus @13:44:01.01 Gate.setIR 1750,5000,0,6 @13:44:01.13 Gate.setIR 0,5000,0,6 @13:44:01.23 PuckHeater.configure :default #ambient 21.3C @13:44:01.32 Gate.power :heat,:ON,limit to 5000mA @13:44:02.04 Mounted lysis Cartridge board#Q05061 @13:44:02.15 Using this cartridge's heat loss curve for HeatingAccelInCarousel @13:44:02.20 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 1" @13:44:02.69 Preparing to filter cartridge 1 @13:44:03.07 Air.in :default @13:44:03.32 Air.seek :sealed @13:44:03.47 Priming sample loop w/10ml, bypass w/10ml @13:44:03.49 SamplePump.setPosition! 0ml @13:44:03.67 Bypass.open @13:44:05.86 Intake.open @13:44:05.89 upsync @13:44:06.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:44:09.37 Vacuum tare is 53% of full scale at 21C (drifted 1%) @13:44:09.56 Abs.in :default @13:44:09.64 Abs.hold @13:44:09.93 SamplePump.in :start @13:44:10.04 SamplePump.seek 10ml,1:10 @13:44:10.62 SamplePump.configure :prime @13:44:22.66 Abs.stop @13:44:22.76 SamplePump.setPosition! 0ml @13:44:22.84 Exhaust.open @13:44:24.19 Bypass.close @13:44:25.68 Abs.in :default @13:44:25.77 Abs.hold @13:44:26.15 SamplePump.in :start @13:44:26.26 SamplePump.seek 10ml,1:10 @13:44:26.84 SamplePump.configure :prime @13:44:38.85 Abs.stop @13:44:38.97 Bypass.open @13:44:39.62 Intake.close @13:44:40.42 Toroid.in :hold @13:44:40.50 Toroid.seek :crack @13:44:42.05 Toroid.seek :cartridge @13:44:43.21 Toroid.in :default @13:44:43.30 Toroid.seek :clear @13:44:45.37 Pressurizing cartridge to 26psi above 15.7psia @13:44:45.39 SamplePump.setPosition! 0ml @13:44:45.48 Intake.open @13:44:46.16 Bypass.close @13:44:46.83 Exhaust.close @13:44:47.60 Abs.in :default @13:44:47.70 Abs.hold @13:44:47.95 SamplePump.in :charge @13:44:48.05 SamplePump.seek 2.3ml,42 seconds @13:45:00.22 SamplePump.stop @13:45:00.32 Intake.close @13:45:00.57 Toroid.in :hold @13:45:00.67 Toroid.seek :bypass @13:45:02.47 Toroid.in :default @13:45:02.56 Toroid.seek :clear @13:45:04.22 Abs.stop @13:45:04.51 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.11ml/s made only -0.4psi vacuum after 0.7ml @13:45:04.54 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 0.66ml" @13:45:04.59 BadNews.email "Serious Leak or Stall -- 0.11ml/s made only -0.4psi vacuum after 0.7ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @13:45:06.78 upsync @13:46:51.95 -> Cartridge.unlock @13:46:52.03 Gate.power :heat,:OFF @13:46:52.07 Gate.power :cartridge,:OFF @13:46:52.22 Carousel.in :default @13:46:52.38 Carousel.home.seek 1 @13:46:52.84 Air.in :unlocking @13:46:53.02 Air.seek :clear @13:46:54.47 Lysis1.in :unlocking @13:46:54.66 Lysis1.seek :clear @13:46:54.71 Plunge.in :unlocking @13:46:54.88 Plunge.seek :clear @13:48:13.87 -> slot 1=>[:dry,:lyseHold_phyto] {1 => { Type: :lyseHold_phyto, State: :dry}} @13:48:20.32 -> fire lyseHold_phyto @13:48:20.50 Selecting Cartridge 1 # @13:48:20.89 Carousel.seek 1 @13:48:21.16 Lysis1.in :fast @13:48:21.24 Lysis1.seek :locked @13:48:23.90 Plunge.in :default @13:48:24.01 Plunge.seek :locked @13:48:24.24 Air.in :fast @13:48:24.32 Air.seek :locked @13:48:25.34 Carousel.reconfigure :locked @13:48:25.54 Powering cartridge bus @13:48:28.23 Gate.setIR 1750,5000,0,6 @13:48:28.36 Gate.setIR 0,5000,0,6 @13:48:28.45 PuckHeater.configure :default #ambient 21.3C @13:48:28.56 Gate.power :heat,:ON,limit to 5000mA @13:48:29.26 Mounted lysis Cartridge board#Q05061 @13:48:29.33 Using this cartridge's heat loss curve for HeatingAccelInCarousel @13:48:29.39 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 1" @13:48:30.31 Preparing to filter cartridge 1 @13:48:31.20 Cmd::Error in detached from preston -- Cannot process while in PRIMING state @13:48:31.28 Air.in :default @13:48:31.35 Waiting to Resume from Checkpoint "Selecting Cartridge 1" @13:48:31.43 Bypass.open @13:48:31.67 Air.seek :sealed @13:48:32.26 GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 1" @13:48:32.84 Toroid.in :hold @13:48:33.13 Toroid.seek :crack @13:48:33.38 upsync @13:48:34.28 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from preston" @13:48:34.89 Toroid.seek :cartridge @13:48:35.21 upsync @13:48:36.26 Toroid.in :default @13:48:36.42 Toroid.seek :clear @13:48:36.88 upsync @13:48:38.36 Exhaust.open @13:48:39.31 Pressurizing cartridge to 26psi above 15.8psia @13:48:39.33 SamplePump.setPosition! 0ml @13:48:39.43 Intake.open @13:48:40.07 Bypass.close @13:48:40.85 Exhaust.close @13:48:41.67 Abs.in :default @13:48:41.77 Abs.hold @13:48:42.03 SamplePump.seek 2.3ml,42 seconds @13:49:02.13 -> Cmd.stop @13:49:02.26 Abs.stop @13:49:02.34 Intake.close @13:49:02.59 Toroid.in :hold @13:49:02.67 Toroid.seek :bypass @13:49:04.33 Toroid.in :default @13:49:04.40 Toroid.seek :clear @13:49:06.25 SamplePump.stop @13:49:06.50 Gate.power :heat,:OFF @13:49:06.54 Gate.power :cartridge,:OFF @13:49:06.72 Carousel.in :default @13:49:06.87 GoodNews.email "After filtering 0.0ml",Subject: "Processing cartridge in slot 1" @13:49:06.98 Cartridge::Error in detached from preston#2 -- Not Mounted @13:49:07.04 Waiting to Resume from Checkpoint "Selecting Cartridge 1" @13:49:07.17 Carousel.home.seek 1 @13:49:07.65 Plunge.seek :locked @13:49:08.12 Air.in :unlocking @13:49:08.38 Air.seek :clear @13:49:08.57 Lysis1.in :unlocking @13:49:08.78 Lysis1.seek :clear @13:49:09.02 BadNews.email "Not Mounted",Subject: "Cartridge::Error in detached from preston#2" @13:49:09.83 upsync @13:49:10.48 Gate.power :analytic1,:OFF @13:49:10.50 Gate.power :motor,:OFF @13:49:10.56 Gate.power :core,:OFF @13:49:10.63 Gate.power :main,:OFF @13:49:10.68 Safely stopped and ready to power off :STOPPED @13:49:11.66 upsync @13:49:34.25 -> ESP.ready! @13:49:34.31 Gate.power :sampler,:OFF,limit to 3500mA @13:49:34.35 Gate.power :skimmer,:OFF,limit to 3500mA @13:49:34.37 Gate.power :main,:ON,limit to 5000mA @13:49:34.42 Gate.power :analytic1,:ON,limit to 4000mA @13:49:34.54 Powering core bus @13:49:37.33 Plunge.configure :default @13:49:37.46 SamplePump.configure :default @13:49:37.60 Toroid.configure :default @13:49:37.74 Carousel.configure :default @13:49:38.72 Abs.configure :default @13:49:38.82 PhaseSeparator.configure :default @13:49:38.93 Archive.configure :default @13:49:39.05 Air.configure :default @13:49:39.13 Lysis1.configure :default @13:49:39.21 Lysis2.configure :default @13:49:39.33 PinPort.configure :default @13:49:39.42 All elf controllers running firmware version 4.92 @13:49:39.46 Gate.power :motor,:ON,limit to 5000mA @13:49:41.80 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:49:41.83 AnalyticModule::Unknown in preston -- SPR @13:49:42.19 Plunge.home.jog 0 @13:49:42.93 Lysis2.home.jog 0 @13:49:42.98 Air.home.jog 0 @13:49:43.05 Carousel.home.jog 0 @13:49:43.10 Lysis1.home.jog 0 @13:49:43.14 Archive.home.jog 0 @13:49:43.19 Toroid.home.jog 0 @13:49:43.25 PinPort.home.jog 0 @13:49:44.04 Plunge.in :homing @13:49:44.49 Plunge.jog 1597 @13:49:44.56 PinPort.in :homing @13:49:44.62 Lysis1.in :homing @13:49:44.67 Archive.in :homing @13:49:44.72 Lysis2.in :homing @13:49:44.76 Toroid.in :homing @13:49:44.80 Air.in :homing @13:49:45.16 PinPort.jog -107 @13:49:45.23 Lysis2.jog 1405 @13:49:45.30 Lysis1.jog 1394 @13:49:45.33 Archive.jog 1396 @13:49:45.41 Air.jog 1376 @13:49:45.45 Toroid.jog 635 @13:49:45.94 PinPort.setRawPosition! 0 @13:49:46.03 PinPort.seek :home @13:49:46.22 PinPort.in :default @13:49:46.28 Plunge.setRawPosition! 50 @13:49:46.41 Plunge.seek :home @13:49:46.71 Plunge.in :default @13:49:46.85 Lysis2.setRawPosition! 50 @13:49:46.87 Lysis1.setRawPosition! 50 @13:49:47.00 Lysis2.seek :home @13:49:47.05 Lysis1.seek :home @13:49:47.28 Air.setRawPosition! 50 @13:49:47.30 Archive.setRawPosition! 50 @13:49:47.48 Air.seek :home @13:49:47.49 Archive.seek :home @13:49:47.74 Lysis1.in :default @13:49:47.77 Lysis2.in :default @13:49:48.00 Archive.in :default @13:49:48.04 Toroid.setRawPosition! 0 @13:49:48.09 Air.in :default @13:49:48.33 Toroid.seek :home @13:49:48.46 Toroid.in :default @13:49:48.62 Toroid.seek :clear @13:49:50.86 Carousel.in :default @13:49:51.01 Carousel.home.seek 1 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 1 Abs = Absolute Pressure (Leak) Sensor @19psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:51:51.85 -> slot 1=>[:dry,:lyseHold_phyto] {1 => { Type: :lyseHold_phyto, State: :dry}} @13:51:53.44 -> slots {1 => { Type: :lyseHold_phyto, State: :dry}, [45,25,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}} @13:51:59.18 -> fire lyseHold_phyto # @13:52:00.21 Toroid.seek :clear @13:52:00.42 Carousel.in :default @13:52:00.55 Carousel.home.seek 1 @13:52:00.86 Selecting Cartridge 1 @13:52:01.18 Carousel.seek 1 @13:52:01.47 Lysis1.in :fast @13:52:01.56 Lysis1.seek :locked @13:52:04.24 Plunge.seek :locked @13:52:04.47 Air.in :fast @13:52:04.56 Air.seek :locked @13:52:05.57 Carousel.reconfigure :locked @13:52:05.77 Powering cartridge bus @13:52:08.43 Gate.setIR 1750,5000,0,6 @13:52:08.57 Gate.setIR 0,5000,0,6 @13:52:08.66 PuckHeater.configure :default #ambient 21.3C @13:52:08.76 Gate.power :heat,:ON,limit to 5000mA @13:52:09.48 Mounted lysis Cartridge board#Q05061 @13:52:09.55 Using this cartridge's heat loss curve for HeatingAccelInCarousel @13:52:09.60 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 1" @13:52:10.01 Preparing to filter cartridge 1 @13:52:10.99 Air.in :default @13:52:11.12 Bypass.open @13:52:11.20 Air.seek :sealed @13:52:12.35 Toroid.in :hold @13:52:12.55 Toroid.seek :crack @13:52:13.17 upsync @13:52:14.20 Toroid.seek :cartridge @13:52:15.34 Toroid.in :default @13:52:15.43 Toroid.seek :clear @13:52:17.27 Exhaust.open @13:52:18.24 Pressurizing cartridge to 26psi above 15.8psia @13:52:18.26 SamplePump.setPosition! 0ml @13:52:18.35 Intake.open @13:52:19.04 Bypass.close @13:52:19.72 Exhaust.close @13:52:20.51 Abs.in :default @13:52:20.60 Abs.hold @13:52:20.86 SamplePump.in :charge @13:52:20.97 SamplePump.seek 2.3ml,42 seconds @13:52:35.92 Abs.stop @13:52:36.03 Intake.close @13:52:36.82 Pressurized cartridge with 1.007ml to 26.2psi @13:52:36.83 Isolated @40.2psia (24.5psi) @13:52:36.86 Verifying cartridge holds at least 0.65..0.85 of 40.2psia @13:52:36.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:52:41.57 Cartridge pressure fell 1% to 40.0psia @13:52:41.70 Sampling 50.0ml @13:52:41.72 SamplePump.setPosition! 0ml @13:52:41.84 Exhaust.open @13:52:42.50 Intake.open @13:52:43.38 Abs.in :default @13:52:43.46 Abs.hold @13:52:43.83 SamplePump.in :start @13:52:43.93 SamplePump.seek 50ml,4:40 @13:52:44.57 SamplePump.configure :sample @13:52:46.93 Cartridge 1 filtering 2/50ml at 0.68ml/s with 22.6psi vacuum @13:53:46.99 Cartridge 1 filtering 41/50ml at 0.65ml/s with 23.2psi vacuum @13:54:01.26 Abs.stop @13:54:01.46 Intake.close @13:54:01.53 Exhaust.close @13:54:01.81 Toroid.in :hold @13:54:01.91 Toroid.seek :bypass @13:54:03.59 Toroid.in :default @13:54:03.70 Toroid.seek :clear @13:54:05.33 Sampled 50.0ml @13:54:05.82 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 1" @13:54:06.45 Handoff.select :waste @13:54:06.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:54:08.38 upsync @13:54:13.29 Syringe tare is 52% of full scale at 21C (drifted 1%) @13:54:13.33 PinPort.in :locking @13:54:13.43 PinPort.seek :locked @13:54:13.97 Archive.in :fast @13:54:14.07 Lysis2.in :fast @13:54:14.20 Archive.seek :full @13:54:14.27 Lysis1.seek 1350uL @13:54:14.50 Lysis2.seek :empty @13:54:22.58 Plunge.seek :reagent @13:54:23.53 Evacuating puck with filter bubble point of 24psi @13:54:23.56 PinPort.seek :locked @13:54:23.71 Handoff.select :waste @13:54:23.75 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:54:30.44 Air syringe pumping up to 1 strokes to reach 4psi @13:54:30.46 Air stroke #1 of 1 @13:54:30.61 Air.in :pressurize @13:54:30.69 Air.seek :pressurized @13:54:36.33 2.0psi after pressurize @13:54:36.36 Air.in :compress @13:54:36.49 Air.seek :evac @13:54:39.54 2.4psi after compress @13:54:50.16 0.4psi after compress (stable) @13:54:50.19 Air.in :default @13:54:50.29 Air.hold @13:54:50.54 at 0.3psi Opened to waste @13:54:50.56 Handoff.select :blocked @13:54:50.75 Archive syringe pumping up to 8 strokes to reach 24psi @13:54:50.76 Archive stroke #1 of 8 @13:54:50.90 Archive.in :pressurize @13:54:50.99 Archive.seek :pressurized @13:55:03.46 2.3psi after pressurize @13:55:19.05 7.9psi after pressurize (stable) @13:55:19.18 Archive.in :compress @13:55:19.28 Archive.seek :empty @13:55:35.02 19.0psi after compress @13:55:45.62 18.9psi after compress (stable) @13:55:45.65 Archive.in :gulp @13:55:45.75 Archive.seek :topVent @13:55:52.08 19.8psi after gulp @13:55:57.69 19.2psi after gulp (stable) @13:55:57.71 Archive stroke #2 of 8 @13:55:57.86 Archive.in :pressurize @13:55:57.94 Archive.seek :pressurized @13:56:10.40 19.6psi after pressurize @13:56:32.49 22.7psi after pressurize (stable) @13:56:32.56 Archive.in :compress @13:56:32.67 Archive.seek :empty @13:56:35.31 Reached 24.0 of 24.0psi after pumping 3179uL (1.84 strokes) @13:56:35.33 Archive.in :default @13:56:35.46 Archive.hold @13:56:35.58 Handoff.select :waste @13:56:35.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:56:52.85 delay 1:00 @13:57:52.97 Handoff.select :blocked @13:57:53.14 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @13:58:15.72 Handoff.select :waste @13:58:15.88 0.3psi at evacEnd @13:58:15.89 Handoff.select :blocked @13:58:16.07 Archive.in :fast @13:58:16.19 Archive.seek :topVent @13:58:23.01 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @13:58:29.61 Stable @0.5psi after Handoff blocked @13:58:29.65 Archive syringe pumping up to 8 strokes to reach 24psi @13:58:29.68 Archive stroke #1 of 8 @13:58:29.84 Archive.in :pressurize @13:58:29.92 Archive.seek :pressurized @13:58:42.38 2.0psi after pressurize @13:58:42.41 Archive.in :compress @13:58:42.52 Archive.seek :empty @13:58:58.27 15.9psi after compress @13:59:08.86 15.2psi after compress (stable) @13:59:08.90 Archive.in :gulp @13:59:09.07 Archive.seek :topVent @13:59:15.40 15.8psi after gulp @13:59:20.99 15.4psi after gulp (stable) @13:59:21.01 Archive stroke #2 of 8 @13:59:21.62 Archive.in :pressurize @13:59:21.80 Archive.seek :pressurized @13:59:34.28 15.7psi after pressurize @13:59:34.31 Archive.in :compress @13:59:34.43 Archive.seek :empty @13:59:44.70 Reached 24.6 of 24.5psi after pumping 3342uL (1.93 strokes) @13:59:44.72 Archive.in :default @13:59:44.84 Archive.hold @13:59:44.95 Handoff.select :waste @13:59:45.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:59:52.21 delay 1:00 @14:00:52.25 Handoff.select :blocked @14:00:52.42 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:01:15.01 Handoff.select :waste @14:01:15.32 0.3psi at evacEnd @14:01:15.36 Air.seek :fullVent @14:01:16.73 Archive.in :fast @14:01:16.82 Archive.seek :topVent @14:01:23.61 Seawater evacuated, Pressure above/below filter released. @14:01:23.74 Lysis1.in :slow @14:01:23.84 Lysis1.seek 1050uL @14:01:53.51 delay 5 seconds @14:01:58.54 Handoff.select :blocked @14:01:58.78 Lysis1.seek 450uL @14:02:57.97 delay 3 seconds @14:03:01.06 Air.in mix with 1.57127 parts Lysis1 @14:03:01.78 Air.seek :evac @14:03:01.82 Lysis1.seek 300uL @14:03:16.84 Lysis1.seek :empty @14:03:46.62 Lysis buffer delivered @21.8psi @14:03:46.64 Polling Can every 2:00 @14:03:47.19 PuckHeater.configure :default #ambient 21.3C @14:03:47.28 PuckHeater.seek 80.6C @14:04:43.06 PuckHeater.hold 2:00,80.6C @14:06:43.04 Polling Can every 2:00 @14:06:43.58 PuckHeater.configure :default #ambient 21.2C @14:06:43.68 PuckHeater.hold 30 seconds,79.6C @14:07:13.85 Polling Can every 2:00 @14:07:14.40 PuckHeater.configure :default #ambient 21.2C @14:07:15.07 PuckHeater.hold 30 seconds,79.1C @14:07:45.25 Polling Can every 2:00 @14:07:45.80 PuckHeater.configure :default #ambient 21.2C @14:07:45.89 PuckHeater.hold 10:00,78.5C @14:17:44.87 Lysis complete. Syringe pressure @27.8psi @14:17:44.90 Archive syringe pumping up to 5 strokes to reach 40psi @14:17:44.94 Archive stroke #1 of 5 @14:17:45.08 Archive.in :pressurize @14:17:45.17 Archive.seek :pressurized @14:17:57.63 27.8psi after pressurize @14:18:03.22 27.2psi after pressurize (stable) @14:18:03.25 Archive.in :compress @14:18:03.38 Archive.seek :empty @14:18:09.60 Reached 40.4 of 40.0psi after pumping 1526uL (0.88 strokes) @14:18:09.63 Archive.in :default @14:18:09.82 Archive.hold @14:18:09.94 delay 2 seconds @14:18:11.97 Archive.in :fast @14:18:12.05 Archive.seek :full @14:18:18.63 Lysis1.seek 16uL @14:18:20.33 delay 1 second @14:18:21.38 Archive.seek :full @14:18:21.42 Lysis1.seek :empty @14:18:23.24 Handoff.select :blocked @14:18:23.44 Initial air pressure @47.6psi @14:18:23.55 Lysis1.in :extract @14:18:23.69 Lysis1.seek 600uL,nil @14:18:27.38 Archive syringe pumping up to 6 strokes to reach 25psi @14:18:27.39 Archive stroke #1 of 6 @14:18:27.55 Archive.in :pressurize @14:18:27.64 Archive.seek :pressurized @14:18:40.16 12.1psi after pressurize @14:18:46.81 Archive.in :default @14:18:46.87 Archive.hold @14:18:47.62 Initial 600uL lysate @14.7psi @14:18:47.76 Handoff.select :blocked @14:18:47.92 Initial air pressure @15.4psi @14:18:48.02 Lysis1.seek 825uL,nil @14:18:48.28 Archive syringe pumping up to 6 strokes to reach 17psi @14:18:48.35 Archive stroke #1 of 6 @14:18:48.56 Archive.in :compress @14:18:48.72 Archive.seek :empty @14:18:49.08 Reached 17.3 of 17.0psi after pumping 1398uL (0.81 strokes) @14:18:49.12 Archive.in :default @14:18:49.21 Archive.hold @14:18:50.20 Archive syringe pumping up to 6 strokes to reach 17psi @14:18:50.22 Archive stroke #1 of 6 @14:18:50.37 Archive.in :compress @14:18:50.51 Archive.seek :empty @14:18:57.41 Archive.in :default @14:18:57.50 Archive.hold @14:18:57.79 Recovered 825uL lysate @16.2psi @14:18:57.82 Air.in :halfSpeed @14:18:57.91 Air.seek :fullVent @14:18:59.95 Puck Top Vented, bottom @16.7psi @14:18:59.96 Handoff.select :waste @14:19:00.14 delay 5 seconds @14:19:05.22 Handoff open to waste, bottom @0.3psi @14:19:05.25 Air.in :default @14:19:05.34 Air.seek :evac @14:19:06.88 Handoff.select :close @14:19:07.07 Gate.power :heat,:OFF @14:19:07.09 Gate.power :cartridge,:OFF @14:19:07.23 PinPort.in :unlocking @14:19:07.32 PinPort.seek :clear @14:19:07.84 Carousel.in :default @14:19:08.00 Carousel.home.seek 1 @14:19:08.36 Plunge.seek :locked @14:19:09.41 Archive.in :unlocking @14:19:09.56 Archive.seek :clear @14:19:09.71 Air.in :unlocking @14:19:09.90 Air.seek :clear @14:19:09.97 Lysis1.in :unlocking @14:19:10.24 Lysis1.seek :clear @14:19:10.41 Lysis2.in :unlocking @14:19:10.51 Lysis2.seek :clear @14:19:15.16 Gate.power :analytic1,:OFF @14:19:15.18 Gate.power :motor,:OFF @14:19:15.21 Gate.power :core,:OFF @14:19:15.26 Gate.power :main,:OFF @14:19:15.30 Safely stopped and ready to power off @14:20:44.23 -> ESP.ready! @14:20:44.30 Gate.power :sampler,:OFF,limit to 3500mA @14:20:44.33 Gate.power :skimmer,:OFF,limit to 3500mA @14:20:44.40 Gate.power :main,:ON,limit to 5000mA @14:20:44.42 Gate.power :analytic1,:ON,limit to 4000mA @14:20:44.55 Powering core bus @14:20:47.35 Plunge.configure :default @14:20:47.46 SamplePump.configure :default @14:20:47.58 Toroid.configure :default @14:20:47.71 Carousel.configure :default @14:20:47.82 Abs.configure :default @14:20:47.94 PhaseSeparator.configure :default @14:20:48.06 Archive.configure :default @14:20:48.21 Air.configure :default @14:20:48.57 Lysis1.configure :default @14:20:48.66 Lysis2.configure :default @14:20:48.77 PinPort.configure :default @14:20:48.86 All elf controllers running firmware version 4.92 @14:20:48.89 Gate.power :motor,:ON,limit to 5000mA @14:20:51.80 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @14:20:51.83 AnalyticModule::Unknown in preston -- SPR @14:20:52.08 Plunge.home.jog 0 @14:20:52.90 PinPort.home.jog 0 @14:20:52.94 Lysis1.home.jog 0 @14:20:53.00 Carousel.home.jog 0 @14:20:53.05 Air.home.jog 0 @14:20:53.11 Archive.home.jog 0 @14:20:53.17 Lysis2.home.jog 0 @14:20:53.23 Toroid.home.jog 0 @14:20:54.00 Plunge.in :homing @14:20:54.36 Plunge.jog 1605 @14:20:54.57 Air.in :homing @14:20:54.68 Lysis2.in :homing @14:20:54.72 Lysis1.in :homing @14:20:54.77 Toroid.in :homing @14:20:54.84 PinPort.in :homing @14:20:54.89 Archive.in :homing @14:20:55.13 Lysis2.jog 1406 @14:20:55.20 Air.jog 1376 @14:20:55.31 Lysis1.jog 1396 @14:20:55.36 PinPort.jog -111 @14:20:55.45 Archive.jog 1403 @14:20:55.60 Toroid.jog 623 @14:20:56.62 PinPort.setRawPosition! 0 @14:20:56.64 Plunge.setRawPosition! 50 @14:20:56.79 PinPort.seek :home @14:20:56.85 Plunge.seek :home @14:20:57.13 PinPort.in :default @14:20:57.16 Lysis2.setRawPosition! 50 @14:20:57.20 Air.setRawPosition! 50 @14:20:57.37 Lysis2.seek :home @14:20:57.53 Air.seek :home @14:20:57.66 Lysis1.setRawPosition! 50 @14:20:57.73 Plunge.in :default @14:20:57.77 Archive.setRawPosition! 50 @14:20:57.96 Lysis1.seek :home @14:20:58.01 Archive.seek :home @14:20:58.30 Lysis2.in :default @14:20:58.33 Air.in :default @14:20:58.56 Archive.in :default @14:20:58.60 Toroid.setRawPosition! 0 @14:20:58.67 Lysis1.in :default @14:20:58.87 Toroid.seek :home @14:20:59.01 Toroid.in :default @14:20:59.15 Toroid.seek :clear @14:21:00.89 Carousel.in :default @14:21:01.06 Carousel.home.seek 1 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 1 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:24:29.71 -> SC.to 11 @14:24:29.74 Carousel.seek 11 Storage Carousel (SC) at 11 @14:25:00.84 -> SC.to 33 @14:25:00.87 Carousel.seek 33 Storage Carousel (SC) at 33 @14:25:32.36 -> exit @14:25:32.40 Session Ended @14:25:37.94

Session Begun @14:26:18.44 -> PhytoSampler.primeVolume=150; PhytoSampler.bypassVolume=50 50 @14:27:08.35 -> slots [11,33]=>[:dry,:lyseHold_phyto] {[33,11] => { Type: :lyseHold_phyto, State: :dry}} @14:27:10.26 -> to 11 @14:27:10.64 Carousel.seek 11 @14:27:15.48 Lysis1.in :fast @14:27:15.57 Lysis1.seek :locked @14:27:18.25 Plunge.seek :locked @14:27:18.44 Air.in :fast @14:27:18.53 Air.seek :locked @14:27:19.91

Carousel.reconfigure :locked @14:27:20.11 Powering cartridge bus @14:27:22.80 Gate.setIR 1750,5000,0,6 @14:27:22.94 Gate.setIR 0,5000,0,6 @14:27:23.03 PuckHeater.configure :default #ambient 21.2C @14:27:23.12 Gate.power :heat,:ON,limit to 5000mA @14:27:23.81 Mounted lysis Cartridge board#Q05075 @14:27:23.88 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @14:32:57.79 -> to 7 @14:32:57.98 Gate.power :heat,:OFF @14:32:58.01 Gate.power :cartridge,:OFF @14:32:58.17 Carousel.in :default @14:32:58.32 Carousel.home.seek 11 @14:32:58.78 Air.in :unlocking @14:32:58.95 Air.seek :clear @14:33:00.00 Lysis1.in :unlocking @14:33:00.17 Lysis1.seek :clear @14:33:00.33 Plunge.in :unlocking @14:33:00.44 Plunge.seek :clear @14:33:01.59

Carousel.seek 7 @14:33:02.76 Lysis1.in :fast @14:33:02.85 Lysis1.seek :locked @14:33:05.51 Plunge.in :default @14:33:05.66 Plunge.seek :locked @14:33:05.78 Air.in :fast @14:33:05.87 Air.seek :locked @14:33:06.88

Carousel.reconfigure :locked @14:33:07.06 Powering cartridge bus @14:33:09.73 Gate.setIR 1750,5000,0,6 @14:33:09.86 Gate.setIR 0,5000,0,6 @14:33:09.96 Gate.power :cartridge,:OFF @14:33:10.55 Mounted archive Cartridge :archive_bac @14:33:19.80 -> delay 5; Lysis1.full @14:33:19.84 delay 5 seconds @14:33:24.89 Lysis1.in :default @14:33:24.98 Lysis1.seek :full Lysis Syringe #1 (LyS1) full @14:33:37.76 -> Cartridge.unlock @14:33:37.90 Carousel.in :default @14:33:38.04 Carousel.home.seek 7 @14:33:38.50 Air.in :unlocking @14:33:38.67 Air.seek :clear @14:33:39.72 Lysis1.in :unlocking @14:33:39.88 Lysis1.seek :clear @14:33:39.97 Plunge.in :unlocking @14:33:40.64 Plunge.seek :clear @14:36:34.80

-> Cartridge.lock @14:36:34.99 Lysis1.in :fast @14:36:35.08 Lysis1.seek :locked @14:36:37.74 Plunge.in :default @14:36:37.88 Plunge.seek :locked @14:36:38.02 Air.in :fast @14:36:38.14 Air.seek :locked @14:36:39.17

Carousel.reconfigure :locked @14:36:39.36 Powering cartridge bus @14:36:42.03 Gate.setIR 1750,5000,0,6 @14:36:42.16 Gate.setIR 0,5000,0,6 @14:36:42.27 Gate.power :cartridge,:OFF @14:36:42.86 Mounted archive Cartridge :archive_bac @14:36:47.73 -> Lysis1.full; Lysis2.full @14:36:47.77 Lysis1.in :default @14:36:47.88 Lysis1.seek :full @14:36:49.62 Lysis2.seek :full Lysis Syringe #2 (LyS2) locked @14:37:34.52 -> PV.clear @14:37:34.57 Plunge.seek :clear Plunge Valve (PV) clear @14:38:10.54 -> Air.clear @14:38:10.59 Air.in :default @14:38:10.69 Air.seek :clear Air Syringe (AirS) clear @14:38:29.57 -> Cartridge.unlock @14:38:29.68 Carousel.in :default @14:38:29.83 Carousel.home.seek 7 @14:38:30.37 Lysis2.in :unlocking @14:38:30.46 Lysis2.seek :clear @14:38:31.64 Lysis1.in :unlocking @14:38:31.78 Lysis1.seek :clear @14:38:59.90

-> slots {[55,1] => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [45,25,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 7 => { Type: :archive_bac, State: :missing}, [33,11] => { Type: :lyseHold_phyto, State: :dry}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}} @14:41:45.45 -> fire lyseHold_phyto, lyseHold_phyto # @14:41:46.48 Toroid.seek :clear @14:41:46.69 Carousel.in :default @14:41:46.85 Carousel.home.seek 7 @14:41:47.14 Selecting Cartridge 33 @14:41:47.47 Carousel.seek 33 @14:41:53.51 Lysis1.in :fast @14:41:53.71 Lysis1.seek :locked @14:41:56.37 Plunge.seek :locked @14:41:56.57 Air.in :fast @14:41:56.66 Air.seek :locked @14:41:57.67 Carousel.reconfigure :locked @14:41:57.85 Powering cartridge bus @14:42:00.53 Gate.setIR 1750,5000,0,6 @14:42:00.66 Gate.setIR 0,5000,0,6 @14:42:00.76 PuckHeater.configure :default #ambient 21.2C @14:42:00.87 Gate.power :heat,:ON,limit to 5000mA @14:42:01.57 Mounted lysis Cartridge board#Q05087 @14:42:01.62 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:42:01.70 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 33" @14:42:02.08 Preparing to filter cartridge 33 @14:42:02.53 Air.in :default @14:42:02.72 Air.seek :sealed @14:42:02.93 Priming sample loop w/150ml, bypass w/50ml @14:42:02.96 SamplePump.setPosition! 0ml @14:42:03.14 Bypass.open @14:42:04.65 Intake.open @14:42:05.42 upsync @14:42:05.57 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:42:08.24 Vacuum tare is 53% of full scale at 21C (drifted 1%) @14:42:08.44 Abs.in :default @14:42:08.54 Abs.hold @14:42:08.82 SamplePump.in :start @14:42:08.93 SamplePump.seek 50ml,3:50 @14:42:09.51 SamplePump.configure :prime @14:43:10.37 Abs.stop @14:43:10.46 SamplePump.setPosition! 0ml @14:43:10.55 Exhaust.open @14:43:12.53 Bypass.close @14:43:14.01 Abs.in :default @14:43:14.11 Abs.hold @14:43:14.48 SamplePump.in :start @14:43:14.59 SamplePump.seek 150ml,10:30 @14:43:15.17 SamplePump.configure :prime @14:46:18.08 Abs.stop @14:46:18.22 Bypass.open @14:46:18.88 Intake.close @14:46:19.66 Toroid.in :hold @14:46:19.75 Toroid.seek :crack @14:46:21.32 Toroid.seek :cartridge @14:46:22.47 Toroid.in :default @14:46:22.56 Toroid.seek :clear @14:46:24.63 Pressurizing cartridge to 26psi above 15.7psia @14:46:24.65 SamplePump.setPosition! 0ml @14:46:24.75 Intake.open @14:46:25.42 Bypass.close @14:46:26.09 Exhaust.close @14:46:26.91 Abs.in :default @14:46:26.99 Abs.hold @14:46:27.24 SamplePump.in :charge @14:46:27.35 SamplePump.seek 2.3ml,42 seconds @14:46:33.91 Abs.stop @14:46:34.02 Intake.close @14:46:34.81 Pressurized cartridge with 0.189ml to 26.2psi @14:46:34.83 Isolated @38.1psia (22.3psi) @14:46:34.87 Verifying cartridge holds at least 0.65..0.85 of 38.1psia @14:46:34.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:46:40.56 Cartridge pressure fell 14% to 32.8psia @14:46:40.69 Sampling 50.0ml @14:46:40.71 SamplePump.setPosition! 0ml @14:46:40.81 Exhaust.open @14:46:41.45 Intake.open @14:46:42.35 Abs.in :default @14:46:42.88 Abs.hold @14:46:43.16 Sea 10.8m deep (30.7psia) @14:46:43.40 SamplePump.in :start @14:46:43.51 SamplePump.seek 50ml,4:40 @14:46:44.09 SamplePump.configure :sample @14:46:45.26 Flow estimate reduced to 0.65ml/s @14:46:45.34 Waiting up to 19 seconds for vacuum to reduce from 30.6 to 12.0psi @14:46:46.60 Cartridge 33 filtering 0/50ml at 0.00ml/s with 29.2psi vacuum @14:47:03.95 Intake.close @14:47:04.05 Exhaust.close @14:47:04.37 Toroid.in :hold @14:47:04.47 Toroid.seek :bypass @14:47:06.19 Toroid.in :default @14:47:06.29 Toroid.seek :clear @14:47:07.95 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:47:07.99 Abs.stop @14:47:08.30 Sampled 0.1ml @14:47:08.78 GoodNews.email "After filtering 0.1ml",Subject: "Processing lyseHold_phyto cartridge in slot 33" @14:47:09.98 Handoff.select :waste @14:47:10.24 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:47:11.71 upsync @14:47:16.87 Syringe tare is 52% of full scale at 21C (drifted 1%) @14:47:16.91 PinPort.in :locking @14:47:17.01 PinPort.seek :locked @14:47:17.54 Archive.in :fast @14:47:17.60 Lysis2.in :fast @14:47:17.71 Lysis1.seek 1350uL @14:47:17.86 Archive.seek :full @14:47:18.00 Lysis2.seek :empty @14:47:26.14 Plunge.seek :reagent @14:47:27.07 Evacuating puck with filter bubble point of 24psi @14:47:27.11 PinPort.seek :locked @14:47:27.25 Handoff.select :waste @14:47:27.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:47:33.91 Air syringe pumping up to 1 strokes to reach 4psi @14:47:33.92 Air stroke #1 of 1 @14:47:34.07 Air.in :pressurize @14:47:34.18 Air.seek :pressurized @14:47:39.83 2.4psi after pressurize @14:47:39.87 Air.in :compress @14:47:39.99 Air.seek :evac @14:47:43.04 1.3psi after compress @14:47:53.64 0.1psi after compress (stable) @14:47:53.73 Air.in :default @14:47:53.84 Air.hold @14:47:54.08 at 0.2psi Opened to waste @14:47:54.11 Handoff.select :blocked @14:47:54.33 Archive syringe pumping up to 8 strokes to reach 24psi @14:47:54.34 Archive stroke #1 of 8 @14:47:54.49 Archive.in :pressurize @14:47:54.58 Archive.seek :pressurized @14:48:07.05 0.8psi after pressurize @14:48:21.14 8.0psi after pressurize (stable) @14:48:21.17 Archive.in :compress @14:48:21.30 Archive.seek :empty @14:48:37.05 15.7psi after compress @14:48:47.64 14.4psi after compress (stable) @14:48:47.76 Archive.in :gulp @14:48:47.87 Archive.seek :topVent @14:48:54.22 15.3psi after gulp @14:48:59.81 14.6psi after gulp (stable) @14:48:59.83 Archive stroke #2 of 8 @14:49:00.08 Archive.in :pressurize @14:49:00.21 Archive.seek :pressurized @14:49:12.66 15.0psi after pressurize @14:49:18.25 14.2psi after pressurize (stable) @14:49:18.28 Archive.in :compress @14:49:18.40 Archive.seek :empty @14:49:27.21 Reached 24.1 of 24.0psi after pumping 3311uL (1.91 strokes) @14:49:27.24 Archive.in :default @14:49:27.33 Archive.hold @14:49:27.45 Handoff.select :waste @14:49:27.62 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:49:34.71 delay 1:00 @14:50:34.85 Handoff.select :blocked @14:50:35.01 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:50:57.60 Handoff.select :waste @14:50:57.76 0.5psi at evacEnd @14:50:57.78 Handoff.select :blocked @14:50:57.95 Archive.in :fast @14:50:58.04 Archive.seek :topVent @14:51:04.74 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:51:11.32 Stable @0.2psi after Handoff blocked @14:51:11.91 Archive syringe pumping up to 8 strokes to reach 24psi @14:51:11.94 Archive stroke #1 of 8 @14:51:12.09 Archive.in :pressurize @14:51:12.20 Archive.seek :pressurized @14:51:24.68 6.0psi after pressurize @14:51:24.71 Archive.in :compress @14:51:24.82 Archive.seek :empty @14:51:40.57 15.7psi after compress @14:51:51.17 14.4psi after compress (stable) @14:51:51.20 Archive.in :gulp @14:51:51.31 Archive.seek :topVent @14:51:57.65 15.2psi after gulp @14:52:03.25 14.6psi after gulp (stable) @14:52:03.26 Archive stroke #2 of 8 @14:52:03.41 Archive.in :pressurize @14:52:03.50 Archive.seek :pressurized @14:52:15.96 15.1psi after pressurize @14:52:15.99 Archive.in :compress @14:52:16.10 Archive.seek :empty @14:52:24.75 Reached 24.3 of 24.2psi after pumping 3307uL (1.91 strokes) @14:52:24.78 Archive.in :default @14:52:24.88 Archive.hold @14:52:24.99 Handoff.select :waste @14:52:25.17 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:52:32.26 delay 1:00 @14:53:32.38 Handoff.select :blocked @14:53:32.55 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:53:55.14 Handoff.select :waste @14:53:55.30 0.3psi at evacEnd @14:53:55.33 Air.seek :fullVent @14:53:56.70 Archive.in :fast @14:53:56.81 Archive.seek :topVent @14:54:03.46 Seawater evacuated, Pressure above/below filter released. @14:54:03.59 Lysis1.in :slow @14:54:03.69 Lysis1.seek 1050uL @14:54:33.36 delay 5 seconds @14:54:38.39 Handoff.select :blocked @14:54:39.20 Lysis1.seek 450uL @14:55:38.39 delay 3 seconds @14:55:41.49 Air.in mix with 1.57127 parts Lysis1 @14:55:42.19 Air.seek :evac @14:55:42.23 Lysis1.seek 300uL @14:55:57.24 Lysis1.seek :empty @14:56:26.96 Lysis buffer delivered @12.3psi @14:56:26.98 Polling Can every 2:00 @14:56:27.56 PuckHeater.configure :default #ambient 21.3C @14:56:27.65 PuckHeater.seek 79.8C @14:56:47.04

-> Syringe.psi 11.7373597472426 @14:56:57.58 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2350 => ["cartridge",:end,:insert,:inserted,:locked]} @14:57:06.95 -> PP.jog 50 @14:57:06.98 PinPort.in :default @14:57:07.08 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 50 counts @14:57:10.80 -> Syringe.psi 12.5650168262261 @14:57:21.75 PuckHeater.hold 2:00,79.8C @14:57:32.16

-> Syringe.psi 13.2697077106179 @14:57:42.87 -> PP.jog -50 @14:57:42.90 PinPort.jog -50 Handoff Pin Port (PP) cartridge @14:57:45.16 -> Syringe.psi 13.3359202769365 @14:57:50.91 -> PP.jog 50 @14:57:50.94 PinPort.jog 50 Handoff Pin Port (PP) at cartridge + 50 counts @14:57:53.55 -> Syringe.psi 13.6055000112341 @14:59:21.95 Polling Can every 2:00 @14:59:22.50 PuckHeater.configure :default #ambient 21.4C @14:59:22.59 PuckHeater.hold 30 seconds,78.7C @14:59:52.84 Polling Can every 2:00 @14:59:53.39 PuckHeater.hold 30 seconds,78.2C @15:00:23.65 Polling Can every 2:00 @15:00:24.20 PuckHeater.configure :default #ambient 21.4C @15:00:24.30 PuckHeater.hold 10:00,77.7C @15:10:24.80 Lysis complete. Syringe pressure @13.6psi @15:10:24.84 Archive syringe pumping up to 5 strokes to reach 40psi @15:10:24.86 Archive stroke #1 of 5 @15:10:25.00 Archive.in :pressurize @15:10:25.08 Archive.seek :pressurized @15:10:37.54 14.0psi after pressurize @15:10:43.64 13.2psi after pressurize (stable) @15:10:43.67 Archive.in :compress @15:10:43.77 Archive.seek :empty @15:10:52.89 Reached 40.3 of 40.0psi after pumping 1588uL (0.92 strokes) @15:10:52.91 Archive.in :default @15:10:53.02 Archive.hold @15:10:53.13 delay 2 seconds @15:10:55.16 Archive.in :fast @15:10:55.24 Archive.seek :full @15:11:01.98 Lysis1.seek 16uL @15:11:03.68 delay 1 second @15:11:04.72 Archive.seek :full @15:11:04.81 Lysis1.seek :empty @15:11:06.58 Handoff.select :blocked @15:11:06.78 Initial air pressure @41.0psi @15:11:06.89 Lysis1.in :extract @15:11:07.03 Lysis1.seek 600uL,nil @15:11:11.79 Archive syringe pumping up to 6 strokes to reach 25psi @15:11:11.81 Archive stroke #1 of 6 @15:11:11.97 Archive.in :pressurize @15:11:12.08 Archive.seek :pressurized @15:11:12.61 Archive.in :default @15:11:12.69 Archive.hold @15:11:13.11 Initial 600uL lysate @14.6psi @15:11:13.13 Handoff.select :blocked @15:11:13.27 Initial air pressure @14.1psi @15:11:13.36 Lysis1.seek 825uL,nil @15:11:13.53 Archive syringe pumping up to 6 strokes to reach 17psi @15:11:13.55 Archive stroke #1 of 6 @15:11:13.75 Archive.in :pressurize @15:11:13.86 Archive.seek :pressurized @15:11:25.91 14.7psi after pressurize @15:11:26.82 Reached 17.2 of 17.0psi after pumping 1397uL (0.81 strokes) @15:11:26.84 Archive.in :default @15:11:27.57 Archive.hold @15:11:29.07 Archive syringe pumping up to 6 strokes to reach 17psi @15:11:29.10 Archive stroke #1 of 6 @15:11:29.35 Archive.in :compress @15:11:29.48 Archive.seek :empty @15:11:29.64 Archive.in :default @15:11:29.71 Archive.hold @15:11:30.06 Recovered 826uL lysate @16.3psi @15:11:30.09 Air.in :halfSpeed @15:11:30.23 Air.seek :fullVent @15:11:32.25 Puck Top Vented, bottom @12.9psi @15:11:32.27 Handoff.select :waste @15:11:32.46 delay 5 seconds @15:11:37.55 Handoff open to waste, bottom @-0.0psi @15:11:37.57 Air.in :default @15:11:37.69 Air.seek :evac @15:11:39.17 Selecting Cartridge 11 @15:11:39.39 Gate.power :heat,:OFF @15:11:39.42 Gate.power :cartridge,:OFF @15:11:39.55 PinPort.in :unlocking @15:11:39.64 PinPort.seek :clear @15:11:40.16 Carousel.in :default @15:11:40.33 Carousel.home.seek 33 @15:11:40.72 Archive.in :unlocking @15:11:40.90 Archive.seek :clear @15:11:41.06 Air.in :unlocking @15:11:41.20 Air.seek :clear @15:11:41.30 Lysis2.in :unlocking @15:11:41.44 Lysis2.seek :clear @15:11:46.01 Lysis1.in :unlocking @15:11:46.18 Lysis1.seek :clear @15:11:46.33 Plunge.in :unlocking @15:11:46.44 Plunge.seek :clear @15:11:49.06 Carousel.seek 11 @15:11:53.92 Lysis1.in :fast @15:11:54.00 Lysis1.seek :locked @15:11:56.66 Plunge.in :default @15:11:56.74 Plunge.seek :locked @15:11:56.94 Air.in :fast @15:11:57.03 Air.seek :locked @15:11:58.03 Carousel.reconfigure :locked @15:11:58.79 Powering cartridge bus @15:12:01.47 Gate.setIR 1750,5000,0,6 @15:12:01.60 Gate.setIR 0,5000,0,6 @15:12:01.71 PuckHeater.configure :default #ambient 21.4C @15:12:01.81 Gate.power :heat,:ON,limit to 5000mA @15:12:02.52 Mounted lysis Cartridge board#Q05075 @15:12:02.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:12:02.64 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11" @15:12:03.08 Preparing to filter cartridge 11 @15:12:03.48 Air.in :default @15:12:03.72 Priming sample loop w/150ml, bypass w/50ml @15:12:03.77 Air.seek :sealed @15:12:03.85 SamplePump.setPosition! 0ml @15:12:04.06 Bypass.open @15:12:05.81 Intake.open @15:12:05.93 upsync @15:12:06.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:12:09.27 Vacuum tare is 53% of full scale at 21C (drifted 1%) @15:12:09.47 Abs.in :default @15:12:09.55 Abs.hold @15:12:09.83 SamplePump.in :start @15:12:09.94 SamplePump.seek 50ml,3:50 @15:12:10.52 SamplePump.configure :prime @15:13:11.35 Abs.stop @15:13:11.54 SamplePump.setPosition! 0ml @15:13:11.65 Exhaust.open @15:13:13.04 Bypass.close @15:13:14.56 Abs.in :default @15:13:14.66 Abs.hold @15:13:15.02 SamplePump.in :start @15:13:15.67 SamplePump.seek 150ml,10:30 @15:13:15.78 SamplePump.configure :prime @15:16:18.97 Abs.stop @15:16:19.09 Bypass.open @15:16:19.76 Intake.close @15:16:20.57 Toroid.in :hold @15:16:20.67 Toroid.seek :crack @15:16:22.28 Toroid.seek :cartridge @15:16:23.44 Toroid.in :default @15:16:23.53 Toroid.seek :clear @15:16:25.58 Pressurizing cartridge to 26psi above 15.7psia @15:16:25.60 SamplePump.setPosition! 0ml @15:16:25.71 Intake.open @15:16:26.39 Bypass.close @15:16:27.08 Exhaust.close @15:16:27.87 Abs.in :default @15:16:27.95 Abs.hold @15:16:28.24 SamplePump.in :charge @15:16:28.35 SamplePump.seek 2.3ml,42 seconds @15:16:43.06 Abs.stop @15:16:43.17 Intake.close @15:16:43.92 Pressurized cartridge with 0.973ml to 26.4psi @15:16:43.94 Isolated @37.7psia (22.1psi) @15:16:43.96 Verifying cartridge holds at least 0.65..0.85 of 37.7psia @15:16:44.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:16:49.68 Cartridge pressure fell 9% to 34.4psia @15:16:49.81 Sampling 50.0ml @15:16:49.84 SamplePump.setPosition! 0ml @15:16:49.96 Exhaust.open @15:16:50.65 Intake.open @15:16:51.54 Abs.in :default @15:16:51.63 Abs.hold @15:16:51.99 SamplePump.in :start @15:16:52.11 SamplePump.seek 50ml,4:40 @15:16:52.68 SamplePump.configure :sample @15:16:55.63 Cartridge 11 filtering 2/50ml at 0.74ml/s with 19.8psi vacuum @15:17:55.15 Cartridge 11 filtering 42/50ml at 0.66ml/s with 17.7psi vacuum 5.3m deep (22.5psia) @15:18:07.68 Abs.stop @15:18:07.87 Intake.close @15:18:07.92 Exhaust.close @15:18:08.09 Handoff.select :close @15:18:08.47 Toroid.in :hold @15:18:08.57 Toroid.seek :bypass @15:18:10.24 Toroid.in :default @15:18:10.35 Toroid.seek :clear @15:18:12.00 Sampled 50.0ml @15:18:12.55 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11" @15:18:13.10 Handoff.select :waste @15:18:13.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:18:16.00 upsync @15:18:19.94 Syringe tare is 52% of full scale at 21C (drifted 1%) @15:18:19.98 PinPort.in :locking @15:18:20.08 PinPort.seek :locked @15:18:20.66 Archive.in :fast @15:18:20.79 Lysis2.in :fast @15:18:20.84 Lysis1.seek 1350uL @15:18:20.88 Archive.seek :full @15:18:21.13 Lysis2.seek :empty @15:18:29.19 Plunge.seek :reagent @15:18:30.17 Evacuating puck with filter bubble point of 24psi @15:18:30.21 PinPort.seek :locked @15:18:30.35 Handoff.select :waste @15:18:30.40 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:18:37.59 Air syringe pumping up to 1 strokes to reach 4psi @15:18:37.61 Air stroke #1 of 1 @15:18:37.74 Air.in :pressurize @15:18:37.83 Air.seek :pressurized @15:18:43.47 2.6psi after pressurize @15:18:43.51 Air.in :compress @15:18:43.63 Air.seek :evac @15:18:45.39 Reached 5.7 of 4.0psi after pumping 1518uL (0.95 strokes) @15:18:45.41 Air.in :default @15:18:45.53 Air.hold @15:18:45.71 Handoff.select :blocked @15:18:45.88 Overpressure in PROCESSING -- Waste port clogged (4.2psi) @15:18:45.90 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 212uL (sensed 210uL)" @15:18:45.95 BadNews.email "Waste port clogged (4.2psi)",Subject: "Overpressure in PROCESSING" @15:18:48.08 upsync @15:29:21.49

-> Handoff Handoff Valve::Manifold selects blocked @15:29:27.25 -> Syringe.psi 8.9836264087387 @15:29:35.15 -> Handoff.waste @15:29:35.17 Handoff.select :waste Handoff Valve::Manifold selects waste @15:30:21.51 -> Cartridge.unlock @15:30:21.58 Gate.power :heat,:OFF @15:30:21.60 Gate.power :cartridge,:OFF @15:30:21.76 PinPort.in :unlocking @15:30:21.85 PinPort.seek :clear @15:30:22.36 Carousel.in :default @15:30:22.52 Carousel.home.seek 11 @15:30:22.92 Archive.in :unlocking @15:30:23.08 Archive.seek :clear @15:30:23.23 Air.in :unlocking @15:30:23.38 Air.seek :clear @15:30:23.54 Lysis2.in :unlocking @15:30:23.63 Lysis2.seek :clear @15:30:28.24 Lysis1.in :unlocking @15:30:28.40 Lysis1.seek :clear @15:30:28.50 Plunge.in :unlocking @15:30:28.63 Plunge.seek :clear @15:30:47.81

-> SC Storage Carousel (SC) at 11 @15:30:49.04 -> to 11 @15:30:49.84 Carousel.seek 11 @15:30:50.25 Lysis1.in :fast @15:30:50.33 Lysis1.seek :locked @15:30:53.02 Plunge.in :default @15:30:53.14 Plunge.seek :locked @15:30:53.29 Air.in :fast @15:30:53.38 Air.seek :locked @15:30:54.38

Carousel.reconfigure :locked @15:30:54.68 Powering cartridge bus @15:30:57.34 Gate.setIR 1750,5000,0,6 @15:30:57.48 Gate.setIR 0,5000,0,6 @15:30:57.59 PuckHeater.configure :default #ambient 21.4C @15:30:57.70 Gate.power :heat,:ON,limit to 5000mA @15:30:58.45 Mounted lysis Cartridge board#Q05075 @15:30:58.52 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @15:31:00.74 -> PhytoSampler.filter 100 @15:31:00.98 Air.in :default @15:31:01.14 Air.seek :sealed @15:31:01.26

Priming sample loop w/150ml, bypass w/50ml @15:31:01.28 SamplePump.setPosition! 0ml @15:31:01.38 Bypass.open @15:31:02.87 Intake.open @15:31:03.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:31:06.32 Vacuum tare is 52% of full scale at 21C (drifted 1%) @15:31:06.49 Abs.in :default @15:31:06.58 Abs.hold @15:31:06.86 SamplePump.in :start @15:31:06.96 SamplePump.seek 50ml,3:50 @15:31:07.55 SamplePump.configure :prime @15:32:08.36

Abs.stop @15:32:08.45 SamplePump.setPosition! 0ml @15:32:08.54 Exhaust.open @15:32:09.90 Bypass.close @15:32:11.42

Abs.in :default @15:32:11.51 Abs.hold @15:32:11.86 SamplePump.in :start @15:32:11.96 SamplePump.seek 150ml,10:30 @15:32:12.55 SamplePump.configure :prime @15:32:31.85

SamplePump.hold @15:32:32.13 Intake.close @15:32:32.26 Exhaust.close @15:32:32.37 Handoff.select :close @15:32:33.10

Abs.stop @15:32:33.31 UserAbort in p -- Sample Pump (SP) seek to 150ml Aborted! @15:32:38.43 -> Cartridge.unlock @15:32:38.50 Gate.power :heat,:OFF @15:32:38.52 Gate.power :cartridge,:OFF @15:32:38.64 Carousel.in :default @15:32:38.79 Carousel.home.seek 11 @15:32:39.25 Air.in :unlocking @15:32:39.39 Air.seek :clear @15:32:40.85 Lysis1.in :unlocking @15:32:41.01 Lysis1.seek :clear @15:32:41.11 Plunge.in :unlocking @15:32:41.23 Plunge.seek :clear @15:38:06.01

-> to 33 @15:38:06.38 Carousel.seek 33 @15:38:11.24 Lysis1.in :fast @15:38:11.31 Lysis1.seek :locked @15:38:13.99 Plunge.in :default @15:38:14.08 Plunge.seek :locked @15:38:14.33 Air.in :fast @15:38:14.42 Air.seek :locked @15:38:15.41

Carousel.reconfigure :locked @15:38:15.61 Powering cartridge bus @15:38:18.27 Gate.setIR 1750,5000,0,6 @15:38:18.41 Gate.setIR 0,5000,0,6 @15:38:18.51 PuckHeater.configure :default #ambient 21.4C @15:38:18.61 Gate.power :heat,:ON,limit to 5000mA @15:38:19.30 Mounted lysis Cartridge board#Q05087 @15:38:19.37 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @15:38:19.52 -> Cartridge.lock @15:38:19.57 Gate.power :heat,:OFF @15:38:19.59 Gate.power :cartridge,:OFF @15:38:21.17 Powering cartridge bus @15:38:23.83 Gate.setIR 1750,5000,0,6 @15:38:23.96 Gate.setIR 0,5000,0,6 @15:38:24.06 PuckHeater.configure :default #ambient 21.4C @15:38:24.18 Gate.power :heat,:ON,limit to 5000mA @15:38:24.87 Mounted lysis Cartridge board#Q05087 @15:38:24.92 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @15:40:44.30 -> Handoff.PP @15:40:44.35 Handoff.select :PP @15:40:44.42 Valve::Manifold::Error in p -- Handoff Valve::Manifold has no selection: PP @15:40:50.89 -> PP.cartridge @15:40:50.95 PinPort.in :locking @15:40:51.04 PinPort.seek :locked Handoff Pin Port (PP) cartridge @15:40:55.56 -> Handoff.waste @15:40:55.59 Handoff.select :waste Handoff Valve::Manifold selects waste @15:41:28.46 -> Lysis1.to 825 @15:41:28.52 Lysis1.in :default @15:41:28.60 Lysis1.seek 825uL Lysis Syringe #1 (LyS1) at 825uL @15:41:50.27 -> Cartridge.unlock @15:41:50.34 Gate.power :heat,:OFF @15:41:50.36 Gate.power :cartridge,:OFF @15:41:50.50 PinPort.in :unlocking @15:41:50.58 PinPort.seek :clear @15:41:51.10 Carousel.in :default @15:41:51.37 Carousel.home.seek 33 @15:41:51.83 Air.in :unlocking @15:41:51.98 Air.seek :clear @15:41:53.00 Lysis1.in :unlocking @15:41:53.16 Lysis1.seek :clear @15:41:53.26 Plunge.in :unlocking @15:41:53.38 Plunge.seek :clear @15:42:54.76

-> Cartridge.lock @15:42:54.96 Lysis1.in :fast @15:42:55.04 Lysis1.seek :locked @15:42:57.70 Plunge.in :default @15:42:57.80 Plunge.seek :locked @15:42:58.01 Air.in :fast @15:42:58.12 Air.seek :locked @15:42:59.15

Carousel.reconfigure :locked @15:42:59.33 Powering cartridge bus @15:43:02.00 Gate.setIR 1750,5000,0,6 @15:43:02.14 Gate.setIR 0,5000,0,6 @15:43:02.23 PuckHeater.configure :default #ambient 21.4C @15:43:02.35 Gate.power :heat,:ON,limit to 5000mA @15:43:03.05 Mounted lysis Cartridge board#Q05087 @15:43:03.15 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @15:43:09.97 -> Handoff.cartridge @15:43:10.00 Handoff.select :cartridge @15:43:10.05 Valve::Manifold::Error in p -- Handoff Valve::Manifold has no selection: cartridge @15:49:11.74 -> PP.cartridge @15:49:11.80 PinPort.in :locking @15:49:11.88 PinPort.seek :locked Handoff Pin Port (PP) cartridge @15:49:15.13 -> Handoff.waste @15:49:15.17 Handoff.select :waste Handoff Valve::Manifold selects waste @15:49:25.13 -> Lysis1.to 825 @15:49:25.18 Lysis1.in :default @15:49:25.26 Lysis1.seek 825uL Lysis Syringe #1 (LyS1) at 825uL @15:49:32.48 -> Lysis2. empty @15:49:32.54 Lysis2.in :default @15:49:32.61 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @15:50:05.37 -> delay 5; Lysis1.push 125, :slow @15:50:05.40 delay 5 seconds @15:50:10.54 Lysis1.in :slow @15:50:10.74 Lysis1.seek 700uL Lysis Syringe #1 (LyS1) at 700uL @15:50:50.70 -> delay 5; Lysis1.push 125, :slow @15:50:50.74 delay 5 seconds @15:50:55.84 Lysis1.seek 575uL Lysis Syringe #1 (LyS1) at 575uL @15:51:18.56 -> delay 5; Lysis1.push 125, :slow @15:51:18.60 delay 5 seconds @15:51:23.70 Lysis1.seek 450uL Lysis Syringe #1 (LyS1) at 450uL @15:52:01.47 -> delay 5; Lysis1.push 125, :slow @15:52:01.49 delay 5 seconds @15:52:06.62 Lysis1.seek 325uL Lysis Syringe #1 (LyS1) at 325uL @16:06:04.63 -> Cartridge.unlock @16:06:04.71 Gate.power :heat,:OFF @16:06:04.73 Gate.power :cartridge,:OFF @16:06:04.87 PinPort.in :unlocking @16:06:04.96 PinPort.seek :clear @16:06:05.48 Carousel.in :default @16:06:05.64 Carousel.home.seek 33 @16:06:06.09 Air.in :unlocking @16:06:06.28 Air.seek :clear @16:06:06.42 Lysis2.in :unlocking @16:06:06.52 Lysis2.seek :clear @16:06:11.08 Lysis1.in :unlocking @16:06:11.26 Lysis1.seek :clear @16:06:11.31 Plunge.in :unlocking @16:06:11.48 Plunge.seek :clear @16:07:18.47

-> SC.to 11 @16:07:18.52 Carousel.seek 11 Storage Carousel (SC) at 11 @16:08:39.46 -> slots {[55,1] => { Type: :lyseHold_phyto, State: :processed, filtered: 50.0, lysed: 825}, [45,25,5] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 7 => { Type: :archive_bac, State: :missing}, 11 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50.0}, 33 => { Type: :lyseHold_phyto, State: :processed, filtered: 0.1, lysed: 826}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}} @16:09:04.40 -> slot 11 => [:dry,:lyseHold_phyto] {11 => { Type: :lyseHold_phyto, State: :dry}} @16:09:19.15 -> fire lyseHold_phyto @16:09:19.34 Selecting Cartridge 11 # @16:09:19.72 Carousel.seek 11 @16:09:19.99 Lysis1.in :fast @16:09:20.07 Lysis1.seek :locked @16:09:22.76 Plunge.in :default @16:09:22.86 Plunge.seek :locked @16:09:23.06 Air.in :fast @16:09:23.15 Air.seek :locked @16:09:24.16 Carousel.reconfigure :locked @16:09:24.36 Powering cartridge bus @16:09:27.02 Gate.setIR 1750,5000,0,6 @16:09:27.16 Gate.setIR 0,5000,0,6 @16:09:27.25 PuckHeater.configure :default #ambient 21.4C @16:09:27.80 Gate.power :heat,:ON,limit to 5000mA @16:09:28.60 Mounted lysis Cartridge board#Q05075 @16:09:28.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:09:28.72 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11" @16:09:29.16 Preparing to filter cartridge 11 @16:09:29.58 Air.in :default @16:09:29.78 Air.seek :sealed @16:09:29.95 Priming sample loop w/150ml, bypass w/50ml @16:09:29.98 SamplePump.setPosition! 0ml @16:09:30.15 Bypass.open @16:09:31.75 upsync @16:09:31.88 Intake.open @16:09:32.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:09:35.27 Vacuum tare is 52% of full scale at 21C (drifted 1%) @16:09:35.46 Abs.in :default @16:09:35.55 Abs.hold @16:09:35.83 SamplePump.in :start @16:09:35.93 SamplePump.seek 50ml,3:50 @16:09:36.52 SamplePump.configure :prime @16:10:37.34 Abs.stop @16:10:37.44 SamplePump.setPosition! 0ml @16:10:37.53 Exhaust.open @16:10:38.89 Bypass.close @16:10:40.43 Abs.in :default @16:10:40.52 Abs.hold @16:10:40.87 SamplePump.in :start @16:10:40.97 SamplePump.seek 150ml,10:30 @16:10:41.56 SamplePump.configure :prime @16:13:44.42 Abs.stop @16:13:44.53 Bypass.open @16:13:45.18 Intake.close @16:13:46.49 Toroid.in :hold @16:13:46.67 Toroid.seek :crack @16:13:48.25 Toroid.seek :cartridge @16:13:49.40 Toroid.in :default @16:13:49.51 Toroid.seek :clear @16:13:51.58 Pressurizing cartridge to 26psi above 15.7psia @16:13:51.60 SamplePump.setPosition! 0ml @16:13:51.71 Intake.open @16:13:52.38 Bypass.close @16:13:53.05 Exhaust.close @16:13:53.86 Abs.in :default @16:13:53.94 Abs.hold @16:13:54.23 SamplePump.in :charge @16:13:54.34 SamplePump.seek 2.3ml,42 seconds @16:14:07.69 Abs.stop @16:14:07.79 Intake.close @16:14:08.56 Pressurized cartridge with 0.798ml to 27.7psi @16:14:08.58 Isolated @36.4psia (20.7psi) @16:14:08.63 Verifying cartridge holds at least 0.65..0.85 of 36.4psia @16:14:08.70 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:14:13.30 Cartridge pressure fell 3% to 35.4psia @16:14:13.43 Sampling 50.0ml @16:14:13.45 SamplePump.setPosition! 0ml @16:14:13.56 Exhaust.open @16:14:14.22 Intake.open @16:14:15.12 Abs.in :default @16:14:15.20 Abs.hold @16:14:15.69 SamplePump.in :start @16:14:15.80 SamplePump.seek 50ml,4:40 @16:14:16.38 SamplePump.configure :sample @16:14:18.79 Cartridge 11 filtering 2/50ml at 0.57ml/s with 24.8psi vacuum @16:15:18.84 Cartridge 11 filtering 41/50ml at 0.65ml/s with 19.6psi vacuum 5.0m deep (22.0psia) @16:15:33.16 Abs.stop @16:15:33.34 Intake.close @16:15:33.45 Exhaust.close @16:15:33.58 Handoff.select :close @16:15:34.41 Toroid.in :hold @16:15:34.59 Toroid.seek :bypass @16:15:36.27 Toroid.in :default @16:15:36.36 Toroid.seek :clear @16:15:38.03 Sampled 50.0ml @16:15:38.52 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11" @16:15:39.26 Gate.power :heat,:OFF @16:15:39.29 Gate.power :cartridge,:OFF @16:15:39.52 Carousel.in :default @16:15:39.85 Carousel.home.seek 11 @16:15:40.18 Carousel.home.seek 11 @16:15:40.88 Plunge.seek :locked @16:15:40.93 Plunge.seek :locked @16:15:41.84 upsync @16:15:41.97 Air.in :unlocking @16:15:42.01 Air.in :unlocking @16:15:42.33 Air.seek :clear @16:15:42.37 Air.seek :clear @16:15:42.70 Lysis1.in :unlocking @16:15:42.76 Lysis1.in :unlocking @16:15:43.00 Lysis1.seek :clear @16:15:43.04 Lysis1.seek :clear @16:15:43.78 Slide::Error in detached from p -- Lysis Syringe #1 (LyS1) aborted at 1589uL (sensed 1595uL) @16:15:43.81 Retry #1 of 2 @16:15:43.86 Lysis1.stop @16:15:44.08 Slide::Error in detached from p#2 -- Air Syringe (AirS) aborted at sealed (sensed 1532uL) @16:15:44.15 Retry #1 of 2 @16:15:44.44 Gate.power :analytic1,:OFF @16:15:44.47 Gate.power :motor,:OFF @16:15:44.52 Gate.power :core,:OFF @16:15:44.56 Gate.power :main,:OFF @16:15:44.64 Gate.power :sampler,:OFF,limit to 3500mA @16:15:44.67 Gate.power :skimmer,:OFF,limit to 3500mA @16:15:44.76 Gate.power :sampler,:OFF,limit to 3500mA @16:15:44.79 Gate.power :skimmer,:OFF,limit to 3500mA @16:15:48.37 Gate.power :main,:ON,limit to 5000mA @16:15:48.40 Gate.power :analytic1,:ON,limit to 4000mA @16:15:49.10 Gate.power :analytic1,:ON,limit to 4000mA @16:15:49.13 Powering core bus @16:15:51.25 Gate.power :core,:OFF @16:15:51.28 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2226 !! @16:15:51.37 Powering core bus @16:15:51.82 BusOff in detached from p -- core bus was not powered while configuring HandoffValves @16:15:51.84 Retry #2 of 2 @16:15:51.92 Gate.power :analytic1,:OFF @16:15:51.95 Gate.power :core,:OFF @16:15:51.99 Gate.power :main,:OFF @16:15:52.07 Gate.power :sampler,:OFF,limit to 3500mA @16:15:52.13 Gate.power :skimmer,:OFF,limit to 3500mA @16:15:52.22 Powering core bus @16:15:54.92 Gate.power :motor,:ON,limit to 5000mA @16:15:54.97 AnalyticModule::Unknown in detached from p#2 -- SPR @16:15:55.12 Slide::Error in detached from p#2 -- Handoff Pin Port (PP) Not yet Homed! @16:15:55.14 Retry #2 of 2 @16:15:55.26 Gate.power :motor,:OFF @16:15:55.32 Gate.power :sampler,:OFF,limit to 3500mA @16:15:55.36 Gate.power :skimmer,:OFF,limit to 3500mA @16:15:55.79 Gate.power :main,:ON,limit to 5000mA @16:15:55.81 Gate.power :analytic1,:ON,limit to 4000mA @16:15:55.93 Gate.power :analytic1,:ON,limit to 4000mA @16:15:56.27 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:15:56.35 Plunge.configure :default @16:15:56.53 SamplePump.configure :default @16:15:56.72 Toroid.configure :default @16:15:56.91 Carousel.configure :default @16:15:57.09 Abs.configure :default @16:15:57.27 PhaseSeparator.configure :default @16:15:57.46 Archive.configure :default @16:15:57.63 Air.configure :default @16:15:57.79 Lysis1.configure :default @16:15:58.24 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2226 !! @16:15:58.34 Gate.power :core,:OFF @16:15:58.37 Lysis2.configure :default @16:15:58.48 BusOff in detached from p#2 -- Lysis Syringe #2 (LyS2) core bus was not powered @16:15:58.55 Powering core bus @16:15:58.64 Gate.power :analytic1,:OFF @16:15:58.66 Gate.power :core,:OFF @16:15:58.70 Gate.power :main,:OFF @16:15:58.75 ready to power off @16:15:59.51 Powering core bus @16:16:02.20 Gate.power :motor,:ON,limit to 5000mA @16:16:02.25 AnalyticModule::Unknown in detached from p -- SPR @16:16:02.41 Slide::Error in detached from p -- Handoff Pin Port (PP) Not yet Homed! @16:16:02.51 Gate.power :motor,:OFF @16:16:02.53 ready to power off @16:16:03.21 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:21:34.34

-> ESP.shutdown [] @16:21:38.84 -> ESP.ready! @16:21:38.92 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @16:21:39.01 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @16:21:39.08 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @16:21:39.17 Slide::Error in p -- Handoff Pin Port (PP) Not yet Homed! @16:21:41.32 upsync @16:21:50.85

-> exit @16:21:50.91 Thread::Aborted in clearToroid -- "Aborted by p" @16:21:50.94 Session Ended @16:21:50.95 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @16:22:02.01 Session Begun @16:22:05.79 -> ESP.ready! @16:22:05.87 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @16:22:05.98 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @16:22:06.03 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @16:22:06.19 Slide::Error in prest -- Handoff Pin Port (PP) Not yet Homed! @16:22:08.71 upsync @16:22:32.99 -> ESP.ready! @16:22:33.07 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @16:22:33.12 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @16:22:33.18 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @16:22:33.38 Slide::Error in prest -- Handoff Pin Port (PP) Not yet Homed! @16:23:30.23 -> MainThread.exit # @16:23:35.15 -> MainThread.exit @16:23:35.17

Sending Email # @16:23:37.95 -> exit @16:23:37.99 Thread::Aborted in clearToroid -- "Aborted by prest" @16:23:38.05 Thread::Aborted in clearToroid -- "Aborted by prest" @16:23:38.07 Session Ended @16:23:38.08 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @16:23:38.15 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @16:23:41.69

Session Begun @16:23:45.16 -> MainThread.exit @16:23:45.19 Email::Error in MAIN -- flush stuck! # @16:23:45.32 ThreadError in p -- killed thread @16:23:45.40 Session Ended @16:23:58.55PDT18-Jul-24

Environmental Sample Processor built 01-Jul-24 (3743e013) @16:24:00.23 >BREAK< ESP3 Gateway v1.43 for core bus @16:24:00.26 >BREAK< ESP3 Gateway v1.43 for cartridge bus @16:24:03.71 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @16:24:04.40 Sea pressure (Abs) tare is 6% of full scale @16:24:09.26 Polling Can every 2:00 @16:24:09.32 Waiting for espclients... @16:24:09.63 Session Begun @16:24:14.63 -> to 11 @16:24:14.76 Slide::Error in preston -- Plunge Valve (PV) Not yet Homed! @16:24:22.22 -> ESP.ready! @16:24:22.28 Gate.power :core,:OFF @16:24:22.31 Gate.power :sampler,:OFF,limit to 3500mA @16:24:22.37 Gate.power :skimmer,:OFF,limit to 3500mA @16:24:26.12 Gate.power :main,:ON,limit to 5000mA @16:24:26.16 Gate.power :analytic1,:ON,limit to 4000mA @16:24:26.36 Powering core bus @16:24:29.14 Plunge.configure :default @16:24:29.25 SamplePump.configure :default @16:24:29.36 Toroid.configure :default @16:24:29.46 Carousel.configure :default @16:24:29.57 Abs.configure :default @16:24:29.69 PhaseSeparator.configure :default @16:24:29.81 Archive.configure :default @16:24:29.92 Air.configure :default @16:24:30.26 Lysis1.configure :default @16:24:30.35 Lysis2.configure :default @16:24:30.46 PinPort.configure :default @16:24:30.54 All elf controllers running firmware version 4.92 @16:24:30.58 Gate.power :motor,:ON,limit to 5000mA @16:24:33.53 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:24:33.56 AnalyticModule::Unknown in preston -- SPR @16:24:34.11 Plunge.home.jog 0 @16:24:34.67 Lysis2.home.jog 0 @16:24:34.70 Air.home.jog 0 @16:24:34.74 Carousel.home.jog 0 @16:24:34.79 Lysis1.home.jog 0 @16:24:34.84 Archive.home.jog 0 @16:24:34.88 Toroid.home.jog 0 @16:24:34.92 PinPort.home.jog 0 @16:24:36.32 Plunge.in :homing @16:24:36.65 Plunge.jog 1593 @16:24:36.74 Lysis2.in :homing @16:24:36.80 Air.in :homing @16:24:36.84 Toroid.in :homing @16:24:36.89 PinPort.in :homing @16:24:36.94 Archive.in :homing @16:24:36.99 Lysis1.in :homing @16:24:37.38 Lysis2.jog 1404 @16:24:37.43 Air.jog 1372 @16:24:37.50 PinPort.jog -109 @16:24:37.54 Toroid.jog 624 @16:24:37.60 Archive.jog 1397 @16:24:37.67 Lysis1.jog 1401 @16:24:38.09 PinPort.setRawPosition! 0 @16:24:38.20 PinPort.seek :home @16:24:38.32 PinPort.in :default @16:24:38.43 Plunge.setRawPosition! 50 @16:24:38.52 Plunge.seek :home @16:24:38.82 Plunge.in :default @16:24:38.93 Lysis2.setRawPosition! 50 @16:24:39.10 Lysis1.setRawPosition! 50 @16:24:39.15 Air.setRawPosition! 50 @16:24:39.19 Lysis2.seek :home @16:24:39.24 Archive.setRawPosition! 50 @16:24:39.46 Lysis1.seek :home @16:24:39.53 Air.seek :home @16:24:39.56 Archive.seek :home @16:24:39.88 Lysis2.in :default @16:24:40.07 Air.in :default @16:24:40.11 Archive.in :default @16:24:40.17 Lysis1.in :default @16:24:40.22 Toroid.setRawPosition! 0 @16:24:40.47 Toroid.seek :home @16:24:40.61 Toroid.in :default @16:24:40.77 Toroid.seek :clear @16:24:42.45 Carousel.in :default @16:24:42.61 Carousel.home.seek 11 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 11 Abs = Absolute Pressure (Leak) Sensor @19psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:24:48.47 -> to 11 @16:24:48.79 Carousel.seek 11 @16:24:49.06 Lysis1.in :fast @16:24:49.14 Lysis1.seek :locked @16:24:51.80 Plunge.seek :locked @16:24:52.02 Air.in :fast @16:24:52.15 Air.seek :locked @16:24:53.16 Carousel.reconfigure :locked @16:24:53.36 Powering cartridge bus @16:24:56.03 Gate.setIR 1750,5000,0,6 @16:24:56.17 Gate.setIR 0,5000,0,6 @16:24:56.26 PuckHeater.configure :default @16:24:56.88 Gate.power :heat,:ON,limit to 5000mA @16:24:57.64 Mounted lysis Cartridge board#Q05075 @16:24:57.70 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @16:25:17.64 -> PhytoSampler.source=nil; PhytoSampler.primeVolume=nil; PhytoSampler.bypassVolume=nil @16:25:25.17 -> Cartridge.dry [] @16:25:47.09 -> slot 11=>[:dry, :lyseHold_phyto] {11 => { Type: :lyseHold_phyto, State: :dry}} @16:25:55.14 -> fire lyseHold_phyto # @16:25:56.19 Toroid.seek :clear @16:25:56.40 Carousel.in :default @16:25:56.55 Carousel.home.seek 11 @16:25:57.04 Carousel.reconfigure :locked @16:25:57.38 Gate.setIR 1750,5000,0,6 @16:25:57.51 Gate.setIR 0,5000,0,6 @16:25:57.61 PuckHeater.configure :default @16:25:57.71 Gate.power :heat,:ON,limit to 5000mA @16:25:58.40 Mounted lysis Cartridge board#Q05075 @16:25:58.46 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:25:58.52 Selecting Cartridge 11 @16:25:58.94 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11" @16:25:59.50 Preparing to filter cartridge 11 @16:25:59.94 Air.in :default @16:26:00.05 Bypass.open @16:26:00.19 Air.seek :sealed @16:26:01.17 Toroid.in :hold @16:26:01.36 Toroid.seek :crack @16:26:01.90 upsync @16:26:03.00 Toroid.seek :cartridge @16:26:04.19 Toroid.in :default @16:26:04.28 Toroid.seek :clear @16:26:06.14 Exhaust.open @16:26:06.84 Intake.open @16:26:07.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:26:10.24 Vacuum tare is 53% of full scale at 21C (drifted 1%) @16:26:10.50 Pressurizing cartridge to 26psi above 15.7psia @16:26:10.52 SamplePump.setPosition! 0ml @16:26:10.65 Bypass.close @16:26:11.36 Exhaust.close @16:26:12.17 Abs.in :default @16:26:12.27 Abs.hold @16:26:12.53 SamplePump.in :charge @16:26:12.64 SamplePump.seek 2.3ml,42 seconds @16:26:22.41 Abs.stop @16:26:22.51 Intake.close @16:26:23.26 Pressurized cartridge with 0.426ml to 26.3psi @16:26:23.27 Isolated @38.4psia (22.7psi) @16:26:23.30 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @16:26:23.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:26:28.00 Cartridge pressure fell 3% to 37.2psia @16:26:28.17 Sampling 50.0ml @16:26:28.19 SamplePump.setPosition! 0ml @16:26:28.39 Exhaust.open @16:26:29.05 Intake.open @16:26:29.92 Abs.in :default @16:26:30.01 Abs.hold @16:26:30.40 SamplePump.in :start @16:26:30.50 SamplePump.seek 50ml,4:40 @16:26:31.09 SamplePump.configure :sample @16:26:33.50 Cartridge 11 filtering 2/50ml at 0.55ml/s with 22.5psi vacuum @16:27:33.56 Cartridge 11 filtering 41/50ml at 0.65ml/s with 27.8psi vacuum @16:27:48.15 Abs.stop @16:27:48.34 Intake.close @16:27:48.46 Exhaust.close @16:27:48.70 Toroid.in :hold @16:27:48.79 Toroid.seek :bypass @16:27:50.47 Toroid.in :default @16:27:50.55 Toroid.seek :clear @16:27:52.21 Sampled 50.0ml @16:27:52.65 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11" @16:27:53.78 Handoff.select :waste @16:27:54.01 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:27:56.50 upsync @16:28:00.65 Syringe tare is 52% of full scale at 21C (drifted 1%) @16:28:00.68 PinPort.in :locking @16:28:00.79 PinPort.seek :locked @16:28:01.33 Archive.in :fast @16:28:01.38 Lysis2.in :fast @16:28:01.51 Lysis1.seek 1350uL @16:28:01.66 Archive.seek :full @16:28:01.80 Lysis2.seek :empty @16:28:09.89 Plunge.seek :reagent @16:28:10.83 Evacuating puck with filter bubble point of 24psi @16:28:10.86 PinPort.seek :locked @16:28:11.01 Handoff.select :waste @16:28:11.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:28:17.66 Air syringe pumping up to 1 strokes to reach 4psi @16:28:17.68 Air stroke #1 of 1 @16:28:17.82 Air.in :pressurize @16:28:17.90 Air.seek :pressurized @16:28:23.56 2.2psi after pressurize @16:28:23.60 Air.in :compress @16:28:23.71 Air.seek :evac @16:28:26.33 Reached 4.3 of 4.0psi after pumping 1568uL (0.98 strokes) @16:28:26.36 Air.in :default @16:28:26.46 Air.hold @16:28:26.63 Handoff.select :blocked @16:28:26.81 Overpressure in PROCESSING -- Waste port clogged (2.5psi) @16:28:26.83 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 162uL (sensed 159uL)" @16:28:26.87 BadNews.email "Waste port clogged (2.5psi)",Subject: "Overpressure in PROCESSING" @16:28:30.95 upsync @16:29:43.72 -> Cartridge.unlock @16:29:43.78 Gate.power :heat,:OFF @16:29:43.80 Gate.power :cartridge,:OFF @16:29:43.95 PinPort.in :unlocking @16:29:44.04 PinPort.seek :clear @16:29:44.58 Carousel.in :default @16:29:44.74 Carousel.home.seek 11 @16:29:45.14 Archive.in :unlocking @16:29:45.31 Archive.seek :clear @16:29:45.38 Air.in :unlocking @16:29:45.61 Air.seek :clear @16:29:45.72 Lysis2.in :unlocking @16:29:45.85 Lysis2.seek :clear @16:29:50.42 Lysis1.in :unlocking @16:29:50.58 Lysis1.seek :clear @16:29:50.68 Plunge.in :unlocking @16:29:50.80 Plunge.seek :clear @16:30:57.15 -> slot 11=>[:dry, :lyseHold_phyto] {11 => { Type: :lyseHold_phyto, State: :dry}} @16:31:06.99 -> Cmd.stop @16:31:07.19 Carousel.in :default @16:31:07.34 Carousel.home.seek 11 @16:31:07.70 Lysis1.in :fast @16:31:07.78 Lysis1.seek :locked @16:31:10.33 Plunge.in :default @16:31:10.44 Plunge.seek :locked @16:31:11.80 Lysis1.in :unlocking @16:31:11.94 Lysis1.seek :clear @16:31:13.46 Gate.power :analytic1,:OFF @16:31:13.49 Gate.power :motor,:OFF @16:31:13.52 Gate.power :core,:OFF @16:31:13.59 Gate.power :main,:OFF @16:31:13.63 Safely stopped and ready to power off :STOPPED @16:31:24.00 -> fire lyseHold_phyto # @16:31:24.26 Gate.power :sampler,:OFF,limit to 3500mA @16:31:24.33 Gate.power :skimmer,:OFF,limit to 3500mA @16:31:24.38 Gate.power :main,:ON,limit to 5000mA @16:31:24.40 Gate.power :analytic1,:ON,limit to 4000mA @16:31:24.50 Powering core bus @16:31:27.28 Plunge.configure :default @16:31:27.41 SamplePump.configure :default @16:31:27.52 Toroid.configure :default @16:31:27.64 Carousel.configure :default @16:31:27.75 Abs.configure :default @16:31:27.85 PhaseSeparator.configure :default @16:31:27.96 Archive.configure :default @16:31:28.06 Air.configure :default @16:31:28.19 Lysis1.configure :default @16:31:28.55 Lysis2.configure :default @16:31:28.65 PinPort.configure :default @16:31:28.75 All elf controllers running firmware version 4.92 @16:31:28.78 Gate.power :motor,:ON,limit to 5000mA @16:31:31.76 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:31:31.79 AnalyticModule::Unknown in detached from preston#2 -- SPR @16:31:32.21 Plunge.home.jog 0 @16:31:33.45 PinPort.home.jog 0 @16:31:33.52 Lysis2.home.jog 0 @16:31:33.60 Air.home.jog 0 @16:31:33.63 Carousel.home.jog 0 @16:31:33.74 Toroid.home.jog 0 @16:31:33.79 Archive.home.jog 0 @16:31:33.86 Lysis1.home.jog 0 @16:31:34.69 Plunge.in :homing @16:31:34.99 Plunge.jog 1604 @16:31:35.21 Lysis2.in :homing @16:31:35.27 Lysis1.in :homing @16:31:35.33 Archive.in :homing @16:31:35.38 PinPort.in :homing @16:31:35.42 Toroid.in :homing @16:31:35.48 Air.in :homing @16:31:35.74 Lysis2.jog 1407 @16:31:35.78 Lysis1.jog 1395 @16:31:35.94 Archive.jog 1400 @16:31:36.18 Air.jog 1376 @16:31:36.23 Toroid.jog 623 @16:31:36.29 PinPort.jog -109 @16:31:36.68 Plunge.setRawPosition! 50 @16:31:36.77 Plunge.seek :home @16:31:36.91 PinPort.setRawPosition! 0 @16:31:37.01 PinPort.seek :home @16:31:37.20 PinPort.in :default @16:31:37.25 Lysis2.setRawPosition! 50 @16:31:37.29 Plunge.in :default @16:31:37.34 Lysis1.setRawPosition! 50 @16:31:37.55 Lysis2.seek :home @16:31:37.62 Lysis1.seek :home @16:31:37.86 Air.setRawPosition! 50 @16:31:37.88 Archive.setRawPosition! 50 @16:31:38.02 Air.seek :home @16:31:38.08 Archive.seek :home @16:31:38.38 Lysis1.in :default @16:31:38.40 Lysis2.in :default @16:31:38.63 Air.in :default @16:31:38.67 Archive.in :default @16:31:38.76 Toroid.setRawPosition! 0 @16:31:38.92 Toroid.seek :home @16:31:39.05 Toroid.in :default @16:31:39.20 Toroid.seek :clear @16:31:40.92 Carousel.in :default @16:31:41.07 Carousel.home.seek 11 @16:31:41.36 Selecting Cartridge 11 @16:31:41.69 Carousel.seek 11 @16:31:41.98 Lysis1.in :fast @16:31:42.06 Lysis1.seek :locked @16:31:44.74 Plunge.seek :locked @16:31:44.95 Air.in :fast @16:31:45.04 Air.seek :locked @16:31:46.49 Carousel.reconfigure :locked @16:31:46.69 Powering cartridge bus @16:31:49.35 Gate.setIR 1750,5000,0,6 @16:31:49.48 Gate.setIR 0,5000,0,6 @16:31:49.57 PuckHeater.configure :default @16:31:49.68 Gate.power :heat,:ON,limit to 5000mA @16:31:50.38 Mounted lysis Cartridge board#Q05087 @16:31:50.43 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:31:50.51 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11" @16:31:50.95 Preparing to filter cartridge 11 @16:31:51.34 Air.in :default @16:31:51.51 Bypass.open @16:31:51.59 Air.seek :sealed @16:31:52.66 Toroid.in :hold @16:31:52.82 Toroid.seek :crack @16:31:53.38 upsync @16:31:54.52 Toroid.seek :cartridge @16:31:55.69 Toroid.in :default @16:31:55.80 Toroid.seek :clear @16:31:57.62 Exhaust.open @16:31:58.33 Intake.open @16:31:59.08 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:32:01.70 Vacuum tare is 53% of full scale at 22C (drifted 1%) @16:32:01.89 Pressurizing cartridge to 26psi above 15.6psia @16:32:01.92 SamplePump.setPosition! 0ml @16:32:02.02 Bypass.close @16:32:02.71 Exhaust.close @16:32:03.49 Abs.in :default @16:32:03.58 Abs.hold @16:32:03.84 SamplePump.in :charge @16:32:03.94 SamplePump.seek 2.3ml,42 seconds @16:32:17.45 Abs.stop @16:32:17.56 Intake.close @16:32:18.35 Pressurized cartridge with 0.816ml to 26.4psi @16:32:18.37 Isolated @37.0psia (21.3psi) @16:32:18.40 Verifying cartridge holds at least 0.65..0.85 of 37.0psia @16:32:18.50 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:32:23.13 Cartridge pressure fell 2% to 36.2psia @16:32:23.27 Sampling 50.0ml @16:32:23.29 SamplePump.setPosition! 0ml @16:32:23.39 Exhaust.open @16:32:24.07 Intake.open @16:32:25.00 Abs.in :default @16:32:25.09 Abs.hold @16:32:25.45 SamplePump.in :start @16:32:25.56 SamplePump.seek 50ml,4:40 @16:32:26.14 SamplePump.configure :sample @16:32:28.56 Cartridge 11 filtering 2/50ml at 0.73ml/s with 17.1psi vacuum 3.9m deep (20.5psia) @16:33:28.61 Cartridge 11 filtering 41/50ml at 0.65ml/s with 21.7psi vacuum @16:33:42.86 Abs.stop @16:33:43.16 Intake.close @16:33:43.23 Exhaust.close @16:33:43.52 Toroid.in :hold @16:33:43.61 Toroid.seek :bypass @16:33:45.30 Toroid.in :default @16:33:45.41 Toroid.seek :clear @16:33:47.06 Sampled 50.0ml @16:33:47.54 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11" @16:33:48.29 Gate.power :heat,:OFF @16:33:48.32 Gate.power :cartridge,:OFF @16:33:48.51 Carousel.in :default @16:33:48.89 Carousel.home.seek 11 @16:33:49.17 Carousel.home.seek 11 @16:33:49.58 Plunge.seek :locked @16:33:49.60 Plunge.seek :locked @16:33:51.37 Air.in :unlocking @16:33:51.47 Air.in :unlocking @16:33:51.70 upsync @16:33:51.80 Air.seek :clear @16:33:51.84 Air.seek :clear @16:33:52.25 Lysis1.in :unlocking @16:33:52.30 Lysis1.in :unlocking @16:33:52.49 Lysis1.seek :clear @16:33:52.52 Lysis1.seek :clear @16:33:52.81 Slide::Error in detached from preston#2 -- Air Syringe (AirS) aborted at 1530uL (sensed 1532uL) @16:33:52.83 Retry #1 of 2 @16:33:52.89 Air.stop @16:33:52.99 Lysis1.stop @16:33:53.27 Slide::Error in detached from preston -- Air Syringe (AirS) aborted at 1895uL (sensed 1896uL) @16:33:53.30 Retry #1 of 2 @16:33:53.48 Gate.power :analytic1,:OFF @16:33:53.51 Gate.power :motor,:OFF @16:33:53.56 Gate.power :core,:OFF @16:33:53.60 Gate.power :main,:OFF @16:33:53.69 Gate.power :sampler,:OFF,limit to 3500mA @16:33:53.71 Gate.power :skimmer,:OFF,limit to 3500mA @16:33:53.84 Gate.power :sampler,:OFF,limit to 3500mA @16:33:53.88 Gate.power :skimmer,:OFF,limit to 3500mA @16:33:57.41 Gate.power :main,:ON,limit to 5000mA @16:33:57.46 Gate.power :analytic1,:ON,limit to 4000mA @16:33:57.56 Powering core bus @16:33:57.60 Gate.power :analytic1,:ON,limit to 4000mA @16:33:59.80 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2380 !! @16:33:59.84 Gate.power :core,:OFF @16:33:59.95 Powering core bus @16:34:00.25 BusOff in detached from preston -- core bus was not powered while configuring HandoffValves @16:34:00.27 Retry #2 of 2 @16:34:00.38 Gate.power :analytic1,:OFF @16:34:00.40 Gate.power :core,:OFF @16:34:00.44 Gate.power :main,:OFF @16:34:00.53 Gate.power :sampler,:OFF,limit to 3500mA @16:34:00.55 Gate.power :skimmer,:OFF,limit to 3500mA @16:34:00.64 Powering core bus @16:34:03.48 Gate.power :motor,:ON,limit to 5000mA @16:34:03.52 AnalyticModule::Unknown in detached from preston#2 -- SPR @16:34:03.68 Slide::Error in detached from preston#2 -- Handoff Pin Port (PP) Not yet Homed! @16:34:03.71 Retry #2 of 2 @16:34:03.82 Gate.power :motor,:OFF @16:34:03.88 Gate.power :sampler,:OFF,limit to 3500mA @16:34:03.92 Gate.power :skimmer,:OFF,limit to 3500mA @16:34:04.25 Gate.power :main,:ON,limit to 5000mA @16:34:04.28 Gate.power :analytic1,:ON,limit to 4000mA @16:34:04.40 Gate.power :analytic1,:ON,limit to 4000mA @16:34:04.71 Plunge.configure :default @16:34:04.81 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:34:04.95 SamplePump.configure :default @16:34:05.13 Toroid.configure :default @16:34:05.30 Carousel.configure :default @16:34:05.49 Abs.configure :default @16:34:05.66 PhaseSeparator.configure :default @16:34:05.84 Archive.configure :default @16:34:06.02 Air.configure :unlocking @16:34:06.20 Lysis1.configure :default @16:34:06.36 Lysis2.configure :default @16:34:06.77 Gate.power :core,:OFF @16:34:06.86 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 2380 !! @16:34:06.95 Powering core bus @16:34:07.01 BusOff in detached from preston#2 -- core bus was not powered while reading Handoff Pin Port (PP)'s status @16:34:07.09 Gate.power :analytic1,:OFF @16:34:07.12 Gate.power :core,:OFF @16:34:07.18 Gate.power :main,:OFF @16:34:07.20 ready to power off @16:34:07.42 Powering core bus @16:34:10.39 Gate.power :motor,:ON,limit to 5000mA @16:34:10.43 AnalyticModule::Unknown in detached from preston -- SPR @16:34:10.59 Slide::Error in detached from preston -- Handoff Pin Port (PP) Not yet Homed! @16:34:10.69 Gate.power :motor,:OFF @16:34:10.71 ready to power off @16:34:11.76 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:35:14.00 -> MainThread.exti @16:35:14.04 NoMethodError in preston -- undefined method `exti' for # @16:35:16.54 -> MainThread.exit # @16:35:16.70 Session Ended @16:35:27.79PDT18-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @16:35:29.45 >BREAK< ESP3 Gateway v1.43 for core bus @16:35:29.48 >BREAK< ESP3 Gateway v1.43 for cartridge bus @16:35:32.98 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @16:35:33.67 Sea pressure (Abs) tare is 6% of full scale @16:35:38.70 Polling Can every 2:00 @16:35:38.75 Waiting for espclients... @16:35:39.19 Session Begun @16:35:41.60 -> slots {[55,45,25,5,1] => { Type: :lyseHold_phyto, State: :processed, filtered: 50, lysed: 825}, 7 => { Type: :archive_bac, State: :missing}, 11 => { Type: :lyseHold_phyto, State: :paused, filtered: 50}, 33 => { Type: :lyseHold_phyto, State: :processed, filtered: 0.1, lysed: 826}, 36 => { Type: :lyseHold_phyto, State: :missing}, 37 => { Type: :lyseHold_phyto, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, was: :processed}} @16:36:20.98 -> slot 11=>[:dry,:lyseHold_phyto] {11 => { Type: :lyseHold_phyto, State: :dry}} @16:36:26.87 -> PP.status @16:36:32.82 -> to 11 @16:36:32.94 Slide::Error in preston -- Plunge Valve (PV) Not yet Homed! @16:36:38.33 -> ESP.ready! @16:36:38.41 Gate.power :core,:OFF @16:36:38.45 Gate.power :sampler,:OFF,limit to 3500mA @16:36:38.47 Gate.power :skimmer,:OFF,limit to 3500mA @16:36:42.24 Gate.power :main,:ON,limit to 5000mA @16:36:42.26 Gate.power :analytic1,:ON,limit to 4000mA @16:36:42.47 Powering core bus @16:36:45.24 Plunge.configure :default @16:36:45.37 SamplePump.configure :default @16:36:45.49 Toroid.configure :default @16:36:45.61 Carousel.configure :default @16:36:45.72 Abs.configure :default @16:36:45.83 PhaseSeparator.configure :default @16:36:45.95 Archive.configure :default @16:36:46.05 Air.configure :default @16:36:46.40 Lysis1.configure :default @16:36:46.49 Lysis2.configure :default @16:36:46.60 PinPort.configure :default @16:36:46.69 All elf controllers running firmware version 4.92 @16:36:46.72 Gate.power :motor,:ON,limit to 5000mA @16:36:49.64 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @16:36:49.67 AnalyticModule::Unknown in preston -- SPR @16:36:50.21 Plunge.home.jog 0 @16:36:50.79 PinPort.home.jog 0 @16:36:50.85 Lysis1.home.jog 0 @16:36:50.89 Carousel.home.jog 0 @16:36:50.94 Air.home.jog 0 @16:36:50.99 Archive.home.jog 0 @16:36:51.03 Lysis2.home.jog 0 @16:36:51.08 Toroid.home.jog 0 @16:36:52.58 Plunge.in :homing @16:36:52.83 Plunge.jog 1600 @16:36:52.91 Lysis2.in :homing @16:36:52.97 Air.in :homing @16:36:53.02 Toroid.in :homing @16:36:53.06 PinPort.in :homing @16:36:53.10 Archive.in :homing @16:36:53.16 Lysis1.in :homing @16:36:53.48 Lysis2.jog 1408 @16:36:53.55 Air.jog 1376 @16:36:53.61 Toroid.jog 609 @16:36:53.67 PinPort.jog -110 @16:36:53.72 Lysis1.jog 1058 @16:36:53.83 Archive.jog 1402 @16:36:54.25 PinPort.setRawPosition! 0 @16:36:54.33 PinPort.seek :home @16:36:54.46 PinPort.in :default @16:36:54.57 Plunge.setRawPosition! 50 @16:36:54.65 Plunge.seek :home @16:36:54.96 Lysis2.setRawPosition! 50 @16:36:55.04 Lysis2.seek :home @16:36:55.18 Lysis1.setRawPosition! 50 @16:36:55.24 Plunge.in :default @16:36:55.29 Air.setRawPosition! 50 @16:36:55.49 Air.seek :home @16:36:55.52 Lysis1.seek :home @16:36:55.76 Lysis2.in :default @16:36:55.78 Archive.setRawPosition! 50 @16:36:55.95 Archive.seek :home @16:36:56.13 Air.in :default @16:36:56.19 Lysis1.in :default @16:36:56.24 Toroid.setRawPosition! 0 @16:36:56.44 Toroid.seek :home @16:36:56.59 Archive.in :default @16:36:56.62 Toroid.in :default @16:36:57.05 Toroid.seek :clear @16:36:58.79 Carousel.in :default @16:36:58.94 Carousel.home.seek 11 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 11 Abs = Absolute Pressure (Leak) Sensor @19psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:38:06.86 -> PhytoSampler.source=PhytoSampler.bypassVolume=PhytoSampler.primeVolume=nil @16:38:15.81 -> fire lyseHold_phyto # @16:38:16.81 Toroid.seek :clear @16:38:17.01 Carousel.in :default @16:38:17.56 Carousel.home.seek 11 @16:38:17.98 Selecting Cartridge 11 @16:38:18.33 Carousel.seek 11 @16:38:18.62 Lysis1.in :fast @16:38:18.70 Lysis1.seek :locked @16:38:21.38 Plunge.seek :locked @16:38:21.59 Air.in :fast @16:38:21.68 Air.seek :locked @16:38:22.68 Carousel.reconfigure :locked @16:38:22.87 Powering cartridge bus @16:38:25.53 Gate.setIR 1750,5000,0,6 @16:38:25.67 Gate.setIR 0,5000,0,6 @16:38:25.77 PuckHeater.configure :default @16:38:25.87 Gate.power :heat,:ON,limit to 5000mA @16:38:26.66 Mounted lysis Cartridge board#Q05087 @16:38:26.72 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:38:26.80 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11" @16:38:27.31 Preparing to filter cartridge 11 @16:38:27.77 Air.in :default @16:38:27.83 Bypass.open @16:38:28.01 Air.seek :sealed @16:38:28.92 Toroid.in :hold @16:38:29.11 Toroid.seek :crack @16:38:29.70 upsync @16:38:30.78 Toroid.seek :cartridge @16:38:31.94 Toroid.in :default @16:38:32.03 Toroid.seek :clear @16:38:33.87 Exhaust.open @16:38:34.64 Intake.open @16:38:35.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:38:38.03 Vacuum tare is 53% of full scale at 21C (drifted 1%) @16:38:38.26 Pressurizing cartridge to 26psi above 15.7psia @16:38:38.86 SamplePump.setPosition! 0ml @16:38:38.96 Bypass.close @16:38:39.62 Exhaust.close @16:38:40.45 Abs.in :default @16:38:40.54 Abs.hold @16:38:40.81 SamplePump.in :charge @16:38:40.90 SamplePump.seek 2.3ml,42 seconds @16:38:53.77 Abs.stop @16:38:53.87 Intake.close @16:38:54.63 Pressurized cartridge with 0.740ml to 26.0psi @16:38:54.65 Isolated @38.0psia (22.3psi) @16:38:54.67 Verifying cartridge holds at least 0.65..0.85 of 38.0psia @16:38:54.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:38:59.37 Cartridge pressure fell 4% to 36.4psia @16:38:59.50 Sampling 50.0ml @16:38:59.52 SamplePump.setPosition! 0ml @16:38:59.62 Exhaust.open @16:39:00.26 Intake.open @16:39:01.15 Abs.in :default @16:39:01.23 Abs.hold @16:39:01.60 SamplePump.in :start @16:39:01.71 SamplePump.seek 50ml,4:40 @16:39:02.29 SamplePump.configure :sample @16:39:04.70 Cartridge 11 filtering 2/50ml at 0.55ml/s with 27.7psi vacuum @16:40:04.76 Cartridge 11 filtering 41/50ml at 0.65ml/s with 20.9psi vacuum @16:40:19.33 Abs.stop @16:40:19.52 Intake.close @16:40:19.59 Exhaust.close @16:40:19.89 Toroid.in :hold @16:40:19.99 Toroid.seek :bypass @16:40:21.65 Toroid.in :default @16:40:21.74 Toroid.seek :clear @16:40:23.40 Sampled 50.0ml @16:40:23.85 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11" @16:40:25.11 Handoff.select :waste @16:40:25.35 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:40:26.84 upsync @16:40:31.98 Syringe tare is 52% of full scale at 22C (drifted 1%) @16:40:32.02 PinPort.in :locking @16:40:32.16 PinPort.seek :locked @16:40:32.70 Archive.in :fast @16:40:32.75 Lysis2.in :fast @16:40:32.87 Lysis1.seek 1350uL @16:40:32.97 Archive.seek :full @16:40:33.10 Lysis2.seek :empty @16:40:41.25 Plunge.seek :reagent @16:40:42.23 Evacuating puck with filter bubble point of 24psi @16:40:42.26 PinPort.seek :locked @16:40:42.40 Handoff.select :waste @16:40:42.44 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:40:50.06 Air syringe pumping up to 1 strokes to reach 4psi @16:40:50.07 Air stroke #1 of 1 @16:40:50.24 Air.in :pressurize @16:40:50.33 Air.seek :pressurized @16:40:55.99 1.6psi after pressurize @16:40:56.02 Air.in :compress @16:40:56.16 Air.seek :evac @16:40:56.46 Reached 5.0 of 4.0psi after pumping 1435uL (0.90 strokes) @16:40:56.48 Air.in :default @16:40:56.59 Air.hold @16:40:56.75 Handoff.select :blocked @16:40:56.93 Overpressure in PROCESSING -- Waste port clogged (3.1psi) @16:40:56.96 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 295uL (sensed 294uL)" @16:40:57.01 BadNews.email "Waste port clogged (3.1psi)",Subject: "Overpressure in PROCESSING" @16:41:00.38 upsync @16:43:06.02 -> MainThread.exit # @16:43:06.32 Session Ended @16:43:27.90PDT18-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @16:43:29.60 >BREAK< ESP3 Gateway v1.43 for core bus @16:43:29.63 >BREAK< ESP3 Gateway v1.43 for cartridge bus @16:43:33.11 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @16:43:33.79 Sea pressure (Abs) tare is 6% of full scale @16:43:38.86 Polling Can every 2:00 @16:43:38.90 Waiting for espclients... @16:43:39.94
 Session Begun
@16:45:55.50 -> Cartridge.unlock
@16:45:55.56 Gate.power :heat,:OFF
@16:45:55.69 PinPort.configure :unlocking
@16:45:55.83 PinPort.seek :clear
@16:45:56.34 Carousel.configure :default
@16:45:56.65 Gate.power :sampler,:OFF,limit to 3500mA
@16:45:56.69 Gate.power :skimmer,:OFF,limit to 3500mA
@16:45:56.73 Gate.power :analytic1,:ON,limit to 4000mA
@16:45:57.20 Gate.power :cartridge,:OFF
@16:45:57.52 Plunge.configure :default
@16:45:57.62 Plunge.hold
@16:45:57.74 Plunge.configure :default
@16:45:57.92 SamplePump.configure :default
@16:45:58.14 Toroid.configure :default
@16:45:58.24 Toroid.hold
@16:45:58.36 Toroid.configure :default
@16:45:58.55 Carousel.hold
@16:45:58.68 Carousel.configure :default
@16:45:59.12 Abs.configure :default
@16:45:59.31 PhaseSeparator.configure :default
@16:45:59.50 Archive.configure :default
@16:46:00.02 Archive.hold
@16:46:00.18 Archive.configure :default
@16:46:00.38 Air.configure :default
@16:46:00.74 Air.hold
@16:46:00.84 Air.configure :default
@16:46:01.03 Lysis1.configure :default
@16:46:01.12 Lysis1.hold
@16:46:01.24 Lysis1.configure :default
@16:46:01.39 Lysis2.configure :default
@16:46:01.49 Lysis2.hold
@16:46:01.61 Lysis2.configure :default
@16:46:01.80 PinPort.in :default
@16:46:01.91 PinPort.hold
@16:46:02.05 PinPort.configure :default
@16:46:02.18 All elf controllers running firmware version 4.92
@16:46:02.25 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK
@16:46:02.30 AnalyticModule::Unknown in pre -- SPR
@16:46:02.33 Carousel.seek 11
@16:46:02.63  Archive.in :unlocking
@16:46:02.76 Archive.seek :clear
@16:46:02.90  Air.in :unlocking
@16:46:03.05 Air.seek :clear
@16:46:03.19  Lysis2.in :unlocking
@16:46:03.28 Lysis2.seek :clear
@16:46:07.85  Lysis1.in :unlocking
@16:46:07.98 Lysis1.seek :clear
@16:46:08.15  Plunge.in :unlocking
@16:46:08.24 Plunge.seek :clear

@16:46:41.54 
 -> Cartridge.lock
@16:46:41.73 Lysis1.in :fast
@16:46:41.82 Lysis1.seek :locked
@16:46:44.48  Plunge.in :default
@16:46:44.61 Plunge.seek :locked
@16:46:44.78  Air.in :fast
@16:46:44.87 Air.seek :locked
@16:46:45.89 
 Carousel.reconfigure :locked
@16:46:46.07 Powering cartridge bus
@16:46:49.73 Gate.setIR 1750,5000,0,6
@16:46:49.85 Gate.setIR 0,5000,0,6
@16:46:49.96 PuckHeater.configure :default
@16:46:50.06 Gate.power :heat,:ON,limit to 5000mA
@16:46:52.83 Mounted lysis Cartridge board#Q05087
@16:46:52.89 Using default cartridge heat loss curve for HeatingAccelInCarousel
:lyseHold_phyto
@16:47:10.29 -> PhytoSampler.source=PhytoSampler.primevolume=PhytoSampler.bypassVolume=nil
@16:47:10.32 NoMethodError in pre -- undefined method `primevolume=' for PhytoSampler:Cartridge::Sampler
@16:47:34.65 -> slot 11=>[:dry, :lyseHold_phyto]
{11 => { Type: :lyseHold_phyto,
  State: :dry}}
@16:47:41.02 -> Cartridge.lock
@16:47:41.08 Gate.power :heat,:OFF
@16:47:41.11 Gate.power :cartridge,:OFF
@16:47:42.69 Powering cartridge bus
@16:47:45.35 Gate.setIR 1750,5000,0,6
@16:47:45.50 Gate.setIR 0,5000,0,6
@16:47:45.59 PuckHeater.configure :default
@16:47:45.68 Gate.power :heat,:ON,limit to 5000mA
@16:47:46.91 Mounted lysis Cartridge board#Q05087
@16:47:46.97 Using default cartridge heat loss curve for HeatingAccelInCarousel
:lyseHold_phyto
@16:47:51.79 -> fire lyseHold_phyto
#
@16:47:52.87  Toroid.seek :clear
@16:47:53.07  Carousel.in :default
@16:47:53.22 Carousel.home.seek 11
@16:47:53.64 Carousel.reconfigure :locked
@16:47:53.89 Gate.setIR 1750,5000,0,6
@16:47:54.02 Gate.setIR 0,5000,0,6
@16:47:54.16 PuckHeater.configure :default
@16:47:54.26 Gate.power :heat,:ON,limit to 5000mA
@16:47:56.95 Mounted lysis Cartridge board#Q05087
@16:47:57.02 Using default cartridge heat loss curve for HeatingAccelInCarousel
@16:47:57.08 Selecting Cartridge 11
@16:47:57.53  GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11"
@16:47:57.90  Preparing to filter cartridge 11
@16:47:58.34 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi
@16:48:00.47  upsync
@16:48:04.96  Intake.close
@16:48:05.06  Exhaust.close
@16:48:05.15  Bypass.close
@16:48:05.49 Scale::Skew::CannotTare in FILTERING -- Intake Head Pressure sensor @31.3% scale out of usable range
@16:48:05.51  Waiting to Resume from Checkpoint "Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi"
@16:48:05.56  BadNews.email "Intake Head Pressure sensor @31.3% scale out of usable range",Subject: "Scale::Skew::CannotTare in FILTERING"
@16:48:08.16  upsync
@16:48:59.88 
 -> PhytoSampler.source=PhytoSampler.primevolume=PhytoSampler.bypassVolume=nil
@16:48:59.93 NoMethodError in pre -- undefined method `primevolume=' for PhytoSampler:Cartridge::Sampler
@16:49:12.09 -> PhytoSampler.source=PhytoSampler.primeVolume=PhytoSampler.bypassVolume=nil

@16:49:14.74 -> fire lyseHold_phyto
@16:49:14.92  Selecting Cartridge 11
#
@16:49:15.41  GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 11"
@16:49:15.85  Preparing to filter cartridge 11
@16:49:16.66  Air.in :default
@16:49:16.75  Bypass.open
@16:49:16.92  Air.seek :sealed
@16:49:17.15 Cmd::Error in detached from pre -- Cannot process while in PRIMING state
@16:49:17.18  Waiting to Resume from Checkpoint "Selecting Cartridge 11"
@16:49:17.75  GoodNews.email "After filtering 0.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11"
@16:49:18.20  Toroid.in :hold
@16:49:18.54 Toroid.seek :crack
@16:49:18.98  upsync
@16:49:19.58  BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from pre"
@16:49:20.25  Toroid.seek :cartridge
@16:49:20.50  upsync
@16:49:21.58  Toroid.in :default
@16:49:21.77 Toroid.seek :clear
@16:49:22.22  upsync
@16:49:23.71  Exhaust.open
@16:49:24.90 Intake.open
@16:49:25.72 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@16:49:28.35 Vacuum tare is 53% of full scale at 21C (drifted 1%)
@16:49:28.53 Pressurizing cartridge to 26psi above 15.6psia
@16:49:28.55 SamplePump.setPosition! 0ml
@16:49:28.66 Bypass.close
@16:49:29.32 Exhaust.close
@16:49:30.14 Abs.in :default
@16:49:30.21 Abs.hold
@16:49:30.50 SamplePump.in :charge
@16:49:30.60 SamplePump.seek 2.3ml,42 seconds
@16:49:43.52 Abs.stop
@16:49:43.62 Intake.close
@16:49:44.38 Pressurized cartridge with 0.746ml to 27.0psi
@16:49:44.39 Isolated @38.5psia (22.9psi)
@16:49:44.42 Verifying cartridge holds at least 0.65..0.85 of 38.5psia
@16:49:44.51 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@16:49:49.12 Cartridge pressure fell 3% to 37.5psia
@16:49:49.26 Sampling 50.0ml
@16:49:49.28 SamplePump.setPosition! 0ml
@16:49:49.40 Exhaust.open
@16:49:50.09 Intake.open
@16:49:51.03 Abs.in :default
@16:49:51.13 Abs.hold
@16:49:51.48 SamplePump.in :start
@16:49:51.59 SamplePump.seek 50ml,4:40
@16:49:52.17  SamplePump.configure :sample
@16:49:54.58  Cartridge 11 filtering 2/50ml at 0.69ml/s with 25.4psi vacuum
@16:50:54.64 Cartridge 11 filtering 40/50ml at 0.64ml/s with 16.2psi vacuum 5.6m deep (23.0psia)
@16:51:09.95  Abs.stop
@16:51:10.16  Intake.close
@16:51:10.21  Exhaust.close
@16:51:10.52  Toroid.in :hold
@16:51:10.63 Toroid.seek :bypass
@16:51:12.30 Toroid.in :default
@16:51:12.41 Toroid.seek :clear
@16:51:14.07 Sampled  50.0ml
@16:51:15.14  GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 11"
@16:51:15.76  Handoff.select :waste
@16:51:15.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:51:17.71  upsync
@16:51:22.63  Syringe tare is 52% of full scale at 22C (drifted 1%)
@16:51:22.66 PinPort.in :locking
@16:51:22.78 PinPort.seek :locked
@16:51:23.32  Archive.in :fast
@16:51:23.38  Lysis2.in :fast
@16:51:23.55  Lysis1.seek 1350uL
@16:51:23.59  Lysis2.seek :empty
@16:51:23.71  Archive.seek :full
@16:51:31.83  Plunge.seek :reagent
@16:51:32.76 Evacuating puck with filter bubble point of 24psi
@16:51:32.80 PinPort.seek :locked
@16:51:32.94 Handoff.select :waste
@16:51:32.98 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:51:39.60 Air syringe pumping up to 1 strokes to reach 8psi
@16:51:39.62 Air stroke #1 of 1
@16:51:39.76 Air.in :pressurize
@16:51:39.83 Air.seek :pressurized
@16:51:45.46 1.3psi after pressurize
@16:51:45.49 Air.in :compress
@16:51:45.61 Air.seek :evac
@16:51:48.66 4.1psi after compress
@16:52:03.25 0.5psi after compress (stable)
@16:52:03.27 Air.in :default
@16:52:03.37 Air.hold
@16:52:03.66 at 0.2psi Opened to waste
@16:52:03.68 Handoff.select :blocked
@16:52:03.86 Archive syringe pumping up to 8 strokes to reach 24psi
@16:52:03.87 Archive stroke #1 of 8
@16:52:04.01 Archive.in :pressurize
@16:52:04.09 Archive.seek :pressurized
@16:52:16.60 1.2psi after pressurize
@16:52:38.69 9.3psi after pressurize (stable)
@16:52:38.72 Archive.in :compress
@16:52:38.83 Archive.seek :empty
@16:52:54.56 18.0psi after compress
@16:53:05.15 16.8psi after compress (stable)
@16:53:05.18 Archive.in :gulp
@16:53:05.33 Archive.seek :topVent
@16:53:11.67 17.8psi after gulp
@16:53:17.26 17.0psi after gulp (stable)
@16:53:17.28 Archive stroke #2 of 8
@16:53:17.42 Archive.in :pressurize
@16:53:17.50 Archive.seek :pressurized
@16:53:29.96 17.7psi after pressurize
@16:53:35.55 17.0psi after pressurize (stable)
@16:53:35.58 Archive.in :compress
@16:53:35.71 Archive.seek :empty
@16:53:42.56 Reached 22.9 of 24.0psi after pumping 3267uL (1.89 strokes)
@16:53:42.58 Archive.in :default
@16:53:42.70 Archive.hold
@16:53:42.80 Handoff.select :waste
@16:53:42.98 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:53:57.60 delay 1:00
@16:54:57.63 Handoff.select :blocked
@16:54:57.90 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@16:55:20.49 Handoff.select :waste
@16:55:20.66 0.6psi at evacEnd
@16:55:20.67 Handoff.select :blocked
@16:55:20.85 Archive.in :fast
@16:55:20.93 Archive.seek :topVent
@16:55:27.50 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi
@16:55:34.10 Stable @0.5psi after Handoff blocked
@16:55:34.17 Archive syringe pumping up to 8 strokes to reach 24psi
@16:55:34.20 Archive stroke #1 of 8
@16:55:34.34 Archive.in :pressurize
@16:55:34.44 Archive.seek :pressurized
@16:55:46.90 5.4psi after pressurize
@16:55:46.93 Archive.in :compress
@16:55:47.04 Archive.seek :empty
@16:56:02.79 16.5psi after compress
@16:56:13.39 15.3psi after compress (stable)
@16:56:13.42 Archive.in :gulp
@16:56:14.00 Archive.seek :topVent
@16:56:20.35 16.2psi after gulp
@16:56:25.94 15.7psi after gulp (stable)
@16:56:25.95 Archive stroke #2 of 8
@16:56:26.12 Archive.in :pressurize
@16:56:26.23 Archive.seek :pressurized
@16:56:38.69 16.1psi after pressurize
@16:56:38.72 Archive.in :compress
@16:56:38.84 Archive.seek :empty
@16:56:47.29 Reached 24.5 of 24.5psi after pumping 3302uL (1.91 strokes)
@16:56:47.32 Archive.in :default
@16:56:47.43 Archive.hold
@16:56:47.54 Handoff.select :waste
@16:56:47.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi
@16:56:56.31 delay 1:00
@16:57:56.34 Handoff.select :blocked
@16:57:56.52 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi
@16:58:19.11 Handoff.select :waste
@16:58:19.27 0.6psi at evacEnd
@16:58:19.30 Air.seek :fullVent
@16:58:20.67 Archive.in :fast
@16:58:20.77 Archive.seek :topVent
@16:58:27.40 Seawater evacuated, Pressure above/below filter released.
@16:58:27.52 Lysis1.in :slow
@16:58:27.68 Lysis1.seek 1050uL
@16:58:57.34 delay 5 seconds
@16:59:02.36 Handoff.select :blocked
@16:59:02.62 Lysis1.seek 450uL
@17:00:01.78 delay 3 seconds
@17:00:04.88 Air.in mix with 1.57127 parts Lysis1
@17:00:05.66  Air.seek :evac
@17:00:05.70  Lysis1.seek 300uL
@17:00:20.71 Lysis1.seek :empty
@17:00:50.45 Lysis buffer delivered @12.8psi
@17:00:50.47 Polling Can every 2:00
@17:00:51.05 PuckHeater.configure :default #ambient 21.4C
@17:00:51.14 PuckHeater.seek 79.8C
@17:01:45.86 PuckHeater.hold 2:00,79.8C
@17:03:46.02 Polling Can every 2:00
@17:03:46.57 PuckHeater.configure :default #ambient 21.4C
@17:03:46.67 PuckHeater.hold 30 seconds,78.7C
@17:04:16.91 Polling Can every 2:00
@17:04:17.46 PuckHeater.configure :default #ambient 21.4C
@17:04:17.55 PuckHeater.hold 30 seconds,78.2C
@17:04:47.79 Polling Can every 2:00
@17:04:48.35 PuckHeater.hold 10:00,77.7C
@17:14:48.74 Lysis complete. Syringe pressure @16.9psi
@17:14:48.87 Archive syringe pumping up to 5 strokes to reach 40psi
@17:14:48.89 Archive stroke #1 of 5
@17:14:49.04 Archive.in :pressurize
@17:14:49.12 Archive.seek :pressurized
@17:15:01.58 17.2psi after pressurize
@17:15:07.69 16.3psi after pressurize (stable)
@17:15:07.72 Archive.in :compress
@17:15:07.82 Archive.seek :empty
@17:15:16.28 Reached 40.2 of 40.0psi after pumping 1574uL (0.91 strokes)
@17:15:16.31 Archive.in :default
@17:15:16.41 Archive.hold
@17:15:16.52 delay 2 seconds
@17:15:18.55 Archive.in :fast
@17:15:18.62 Archive.seek :full
@17:15:25.35 Lysis1.seek 16uL
@17:15:27.06 delay 1 second
@17:15:28.11  Archive.seek :full
@17:15:28.18  Lysis1.seek :empty
@17:15:29.96 Handoff.select :blocked
@17:15:30.18 Initial air pressure @42.1psi
@17:15:30.31 Lysis1.in :extract
@17:15:30.43 Lysis1.seek 600uL,nil
@17:15:34.82  Archive syringe pumping up to 6 strokes to reach 25psi
@17:15:34.83 Archive stroke #1 of 6
@17:15:34.98 Archive.in :pressurize
@17:15:35.10 Archive.seek :pressurized
@17:15:36.02 Syringe::LysatePlungerStuck in PROCESSING -- at < 506uL
@17:15:36.07  Lysis1.in :default
@17:15:36.22 Lysis1.seek 471uL
@17:15:47.59  16.8psi after pressurize
@17:16:01.69 24.7psi after pressurize (stable)
@17:16:01.72 Archive.in :compress
@17:16:01.90 Archive.seek :empty
@17:16:02.18 Reached 25.9 of 25.0psi after pumping 1398uL (0.81 strokes)
@17:16:02.20 Archive.in :default
@17:16:02.29 Archive.hold
@17:16:02.48  Lysis1.in :extract
@17:16:02.64 Lysis1.seek 600uL,nil
@17:16:04.23 Initial 600uL lysate @21.3psi
@17:16:04.25 Handoff.select :blocked
@17:16:04.40 Initial air pressure @21.2psi
@17:16:04.51 Lysis1.seek 825uL,nil
@17:16:06.93 Recovered 825uL lysate @16.0psi
@17:16:06.96 Air.in :halfSpeed
@17:16:07.07 Air.seek :fullVent
@17:16:09.61 Puck Top Vented, bottom @12.1psi
@17:16:09.62 Handoff.select :waste
@17:16:09.82 delay 5 seconds
@17:16:14.90 Handoff open to waste, bottom @0.3psi
@17:16:14.93 Air.in :default
@17:16:15.03 Air.seek :evac
@17:16:16.68  Handoff.select :close
@17:16:16.87  Gate.power :heat,:OFF
@17:16:16.89 Gate.power :cartridge,:OFF
@17:16:17.03 PinPort.in :unlocking
@17:16:17.12 PinPort.seek :clear
@17:16:17.64 Carousel.in :default
@17:16:17.79 Carousel.home.seek 11
@17:16:18.18 Plunge.seek :locked
@17:16:19.11  Archive.in :unlocking
@17:16:19.24 Archive.seek :clear
@17:16:19.41  Air.in :unlocking
@17:16:19.58 Air.seek :clear
@17:16:19.65  Lysis1.in :unlocking
@17:16:19.85 Lysis1.seek :clear
@17:16:20.00  Lysis2.in :unlocking
@17:16:20.12 Lysis2.seek :clear
@17:16:24.78  Gate.power :analytic1,:OFF
@17:16:24.80 Gate.power :motor,:OFF
@17:16:24.85 Gate.power :core,:OFF
@17:16:24.88 Gate.power :main,:OFF
@17:16:24.91 Safely stopped and ready to power off
@17:16:42.56 
 -> ESP.ready!
@17:16:42.63 Gate.power :sampler,:OFF,limit to 3500mA
@17:16:42.65 Gate.power :skimmer,:OFF,limit to 3500mA
@17:16:42.69 Gate.power :main,:ON,limit to 5000mA
@17:16:42.74 Gate.power :analytic1,:ON,limit to 4000mA
@17:16:42.87 Powering core bus
@17:16:45.66 Plunge.configure :default
@17:16:45.77 SamplePump.configure :default
@17:16:45.89 Toroid.configure :default
@17:16:46.02 Carousel.configure :default
@17:16:46.16 Abs.configure :default
@17:16:46.26 PhaseSeparator.configure :default
@17:16:46.38 Archive.configure :default
@17:16:46.50 Air.configure :default
@17:16:46.85 Lysis1.configure :default
@17:16:46.94 Lysis2.configure :default
@17:16:47.05 PinPort.configure :default
@17:16:47.13 All elf controllers running firmware version 4.92
@17:16:47.16 Gate.power :motor,:ON,limit to 5000mA
@17:16:50.13 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK
@17:16:50.17 AnalyticModule::Unknown in pre -- SPR
@17:16:50.59  Toroid.home.jog 0
@17:16:50.69  Plunge.home.jog 0
@17:16:51.40  Lysis2.home.jog 0
@17:16:51.45  Air.home.jog 0
@17:16:51.48  Carousel.home.jog 0
@17:16:51.56  Lysis1.home.jog 0
@17:16:51.60  Archive.home.jog 0
@17:16:51.65  PinPort.home.jog 0
@17:16:52.54  Toroid.in :homing
@17:16:52.60  Plunge.in :homing
@17:16:52.80 Plunge.jog 1596
@17:16:52.83  Toroid.jog 612
@17:16:53.61  PinPort.in :homing
@17:16:53.67  Lysis2.in :homing
@17:16:53.72  Air.in :homing
@17:16:53.77  Archive.in :homing
@17:16:53.82  Lysis1.in :homing
@17:16:54.20  PinPort.jog -109
@17:16:54.24  Air.jog 1375
@17:16:54.36  Lysis2.jog 1404
@17:16:54.40  Lysis1.jog 1400
@17:16:54.46  Archive.jog 1405
@17:16:54.89  PinPort.setRawPosition! 0
@17:16:54.92  Plunge.setRawPosition! 50
@17:16:55.08  PinPort.seek :home
@17:16:55.10  Plunge.seek :home
@17:16:55.38  PinPort.in :default
@17:16:55.40  Toroid.setRawPosition! 0
@17:16:55.54 Toroid.seek :home
@17:16:55.69  Plunge.in :default
@17:16:55.71  Toroid.in :default
@17:16:55.94  Lysis1.setRawPosition! 50
@17:16:56.00  Air.setRawPosition! 50
@17:16:56.04  Archive.setRawPosition! 50
@17:16:56.07  Lysis2.setRawPosition! 50
@17:16:56.33  Lysis1.seek :home
@17:16:56.40  Air.seek :home
@17:16:56.46  Archive.seek :home
@17:16:56.65  Lysis2.seek :home
@17:16:56.94  Air.in :default
@17:16:56.97  Lysis1.in :default
@17:16:57.19  Archive.in :default
@17:16:57.21  Lysis2.in :default
@17:16:57.64  Toroid.seek :clear
@17:16:59.32 
 Carousel.in :default
@17:16:59.47 Carousel.home.seek 11
Plunge = Plunge Valve (PV) clear
Handoff Valve::Manifold selects blocked
SamplePump = Sample Pump (SP) Not yet Homed!
Intake is closed
Exhaust is closed
Bypass = Bypass (PB) is closed
Toroid = Toroid Valve (TV) clear
Carousel = Storage Carousel (SC) at 11
Abs = Absolute Pressure (Leak) Sensor @19psia
PhaseSeparator = Intake Head Pressure @2.18psi
Archive = Archive Syringe (ArS) clear
Air = Air Syringe (AirS) clear
Lysis1 = Lysis Syringe #1 (LyS1) clear
Lysis2 = Lysis Syringe #2 (LyS2) clear
PinPort = Handoff Pin Port (PP) clear
@08:32:48.62PDT22-Jul-24 
Environmental Sample Processor built 01-Jul-24 (3743e013) @08:32:50.39 >BREAK< ESP3 Gateway v1.43 for core bus @08:32:50.41 >BREAK< ESP3 Gateway v1.43 for cartridge bus @08:32:53.85 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @08:32:54.68 Sea pressure (Abs) tare is 6% of full scale @08:32:59.64 Polling Can every 2:00 @08:32:59.70 Waiting for espclients... @08:33:00.49 Session Begun @08:33:08.67 -> to 8 @08:33:08.99 Carousel.configure :default @08:33:09.09 Carousel.seek 8 @08:33:10.05 Lysis1.configure :fast @08:33:10.15 Lysis1.seek :locked @08:33:12.79 Plunge.configure :default @08:33:12.96 Plunge.seek :locked @08:33:13.07 Air.configure :fast @08:33:13.17 Air.seek :locked @08:33:14.17 Carousel.reconfigure :locked @08:33:14.45 Gate.power :sampler,:OFF,limit to 3500mA @08:33:14.47 Gate.power :skimmer,:OFF,limit to 3500mA @08:33:14.52 Gate.power :analytic1,:ON,limit to 4000mA @08:33:15.24 Plunge.hold @08:33:15.36 Plunge.configure :default @08:33:15.53 SamplePump.configure :default @08:33:15.72 Toroid.configure :default @08:33:15.82 Toroid.hold @08:33:15.93 Toroid.configure :default @08:33:16.89 Carousel.hold @08:33:17.03 Carousel.configure :locked @08:33:17.22 Abs.configure :default @08:33:17.40 PhaseSeparator.configure :default @08:33:17.58 Archive.configure :default @08:33:17.76 Air.in :default @08:33:17.85 Air.hold @08:33:17.99 Air.configure :default @08:33:18.14 Lysis1.in :default @08:33:18.24 Lysis1.hold @08:33:18.64 Lysis1.configure :default @08:33:18.79 Lysis2.configure :default @08:33:18.97 PinPort.configure :default @08:33:19.06 All elf controllers running firmware version 4.92 @08:33:20.09 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:33:20.12 AnalyticModule::Unknown in preston -- SPR @08:33:20.36 Powering cartridge bus @08:33:23.03 Gate.setIR 1750,5000,0,6 @08:33:23.17 Gate.setIR 0,5000,0,6 @08:33:23.28 PuckHeater.configure :default @08:33:23.38 Gate.power :heat,:ON,limit to 5000mA @08:33:24.17 Mounted lysis Cartridge board#Q05092 @08:33:24.26 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @08:33:35.91 -> PhytoSampler.primeVolume=BACsampler.bypassVolume=30 30 @08:33:41.99 -> PhytoSampler.source=nil @08:33:49.54 -> fire lyseHold_phyto # @08:33:50.50 Toroid.seek :clear @08:33:50.70 Carousel.in :default @08:33:50.85 Carousel.home.seek 8 @08:33:51.27 Carousel.reconfigure :locked @08:33:51.51 Gate.setIR 1750,5000,0,6 @08:33:51.66 Gate.setIR 0,5000,0,6 @08:33:51.75 PuckHeater.configure :default @08:33:51.85 Gate.power :heat,:ON,limit to 5000mA @08:33:54.53 Mounted lysis Cartridge board#Q05092 @08:33:54.60 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:33:54.70 Selecting Cartridge 8 @08:33:55.09 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 8" @08:33:55.46 Preparing to filter cartridge 8 @08:33:56.37 Air.seek :sealed @08:33:56.60 Priming sample loop w/30ml, bypass w/50ml @08:33:56.66 SamplePump.setPosition! 0ml @08:33:56.79 Exhaust.close @08:33:56.99 Bypass.open @08:33:58.64 Intake.open @08:33:59.32 upsync @08:33:59.52 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:34:02.16 Vacuum tare is 52% of full scale at 19C (drifted 0%) @08:34:02.40 Abs.in :default @08:34:02.49 Abs.hold @08:34:02.77 SamplePump.in :start @08:34:02.88 SamplePump.seek 50ml,3:50 @08:34:03.46 SamplePump.configure :prime @08:35:04.29 Abs.stop @08:35:04.44 SamplePump.setPosition! 0ml @08:35:04.59 Exhaust.open @08:35:06.01 Bypass.close @08:35:07.53 Abs.in :default @08:35:07.62 Abs.hold @08:35:07.97 SamplePump.in :start @08:35:08.07 SamplePump.seek 30ml,2:30 @08:35:08.66 SamplePump.configure :prime @08:35:45.06 Abs.stop @08:35:45.18 Bypass.open @08:35:45.88 Intake.close @08:35:46.73 Toroid.in :hold @08:35:46.81 Toroid.seek :crack @08:35:48.40 Toroid.seek :cartridge @08:35:49.56 Toroid.in :default @08:35:49.66 Toroid.seek :clear @08:35:51.73 Pressurizing cartridge to 26psi above 15.8psia @08:35:51.75 SamplePump.setPosition! 0ml @08:35:51.84 Intake.open @08:35:52.56 Bypass.close @08:35:53.24 Exhaust.close @08:35:54.03 Abs.in :default @08:35:54.59 Abs.hold @08:35:54.85 SamplePump.in :charge @08:35:55.04 SamplePump.seek 2.3ml,42 seconds @08:36:09.79 Abs.stop @08:36:09.89 Intake.close @08:36:10.66 Pressurized cartridge with 0.978ml to 27.3psi @08:36:10.68 Isolated @36.9psia (21.1psi) @08:36:10.70 Verifying cartridge holds at least 0.65..0.85 of 36.9psia @08:36:10.78 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:36:15.40 Cartridge pressure fell 4% to 35.6psia @08:36:15.53 Sampling 50.0ml @08:36:15.55 SamplePump.setPosition! 0ml @08:36:15.66 Exhaust.open @08:36:16.34 Intake.open @08:36:17.20 Abs.in :default @08:36:17.29 Abs.hold @08:36:17.63 SamplePump.in :start @08:36:17.75 SamplePump.seek 50ml,4:40 @08:36:18.32 SamplePump.configure :sample @08:36:20.73 Cartridge 8 filtering 2/50ml at 0.57ml/s with 25.3psi vacuum @08:36:56.40 -> Handoff.waste @08:36:56.44 Handoff.select :waste Handoff Valve::Manifold selects waste @08:37:20.78 Cartridge 8 filtering 43/50ml at 0.68ml/s with 17.9psi vacuum @08:37:31.52 Abs.stop @08:37:31.71 Intake.close @08:37:31.79 Exhaust.close @08:37:31.90 Handoff.select :close @08:37:32.17 Toroid.in :hold @08:37:32.30 Toroid.seek :bypass @08:37:33.98 Toroid.in :default @08:37:34.07 Toroid.seek :clear @08:37:35.70 Sampled 50.0ml @08:37:36.18 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 8" @08:37:36.73 Handoff.select :waste @08:37:36.91 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:37:40.08 upsync @08:37:43.53 Syringe tare is 51% of full scale at 20C (drifted -0%) @08:37:43.56 PinPort.in :locking @08:37:43.67 PinPort.seek :locked @08:37:44.19 Archive.in :fast @08:37:44.30 Lysis2.in :fast @08:37:44.41 Lysis1.in :fast @08:37:44.55 Archive.seek :full @08:37:44.62 Lysis2.seek :empty @08:37:44.67 Lysis1.seek 1350uL @08:37:52.84 Plunge.seek :reagent @08:37:53.77 Evacuating puck with filter bubble point of 24psi @08:37:53.80 PinPort.seek :locked @08:37:53.96 Handoff.select :waste @08:37:54.01 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:38:00.62 Air syringe pumping up to 1 strokes to reach 8psi @08:38:00.63 Air stroke #1 of 1 @08:38:00.78 Air.in :pressurize @08:38:00.86 Air.seek :pressurized @08:38:06.50 1.9psi after pressurize @08:38:06.53 Air.in :compress @08:38:06.65 Air.seek :evac @08:38:09.68 2.3psi after compress @08:38:29.27 0.1psi after compress (stable) @08:38:29.29 Air.in :default @08:38:29.41 Air.hold @08:38:29.71 at -0.0psi Opened to waste @08:38:29.73 Handoff.select :blocked @08:38:29.92 Archive syringe pumping up to 8 strokes to reach 24psi @08:38:29.93 Archive stroke #1 of 8 @08:38:30.08 Archive.in :pressurize @08:38:30.18 Archive.seek :pressurized @08:38:42.68 0.9psi after pressurize @08:38:48.30 0.3psi after pressurize (stable) @08:38:48.32 Archive.in :compress @08:38:48.45 Archive.seek :empty @08:39:04.20 16.7psi after compress @08:39:15.27 15.8psi after compress (stable) @08:39:15.29 Archive.in :gulp @08:39:15.52 Archive.seek :topVent @08:39:21.87 16.8psi after gulp @08:39:27.45 16.0psi after gulp (stable) @08:39:27.47 Archive stroke #2 of 8 @08:39:27.62 Archive.in :pressurize @08:39:27.70 Archive.seek :pressurized @08:39:40.16 16.6psi after pressurize @08:39:49.75 20.0psi after pressurize (stable) @08:39:49.77 Archive.in :compress @08:39:49.88 Archive.seek :empty @08:39:56.36 Reached 24.1 of 24.0psi after pumping 3260uL (1.88 strokes) @08:39:56.38 Archive.in :default @08:39:56.50 Archive.hold @08:39:56.62 Handoff.select :waste @08:39:56.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:40:15.88 delay 1:00 @08:41:15.91 Handoff.select :blocked @08:41:16.26 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @08:41:38.85 Handoff.select :waste @08:41:39.01 0.4psi at evacEnd @08:41:39.02 Handoff.select :blocked @08:41:39.20 Archive.in :fast @08:41:39.29 Archive.seek :topVent @08:41:45.90 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @08:41:52.49 Stable @0.1psi after Handoff blocked @08:41:52.52 Archive syringe pumping up to 8 strokes to reach 24psi @08:41:52.57 Archive stroke #1 of 8 @08:41:52.71 Archive.in :pressurize @08:41:52.79 Archive.seek :pressurized @08:42:05.25 7.3psi after pressurize @08:42:05.28 Archive.in :compress @08:42:05.39 Archive.seek :empty @08:42:21.14 15.8psi after compress @08:42:31.73 14.6psi after compress (stable) @08:42:31.75 Archive.in :gulp @08:42:31.88 Archive.seek :topVent @08:42:38.20 15.4psi after gulp @08:42:43.83 14.7psi after gulp (stable) @08:42:43.84 Archive stroke #2 of 8 @08:42:43.98 Archive.in :pressurize @08:42:44.07 Archive.seek :pressurized @08:42:56.53 15.0psi after pressurize @08:42:57.00 Archive.in :compress @08:42:57.13 Archive.seek :empty @08:43:06.39 Reached 22.9 of 24.1psi after pumping 3318uL (1.92 strokes) @08:43:06.41 Archive.in :default @08:43:06.54 Archive.hold @08:43:06.65 Handoff.select :waste @08:43:06.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:43:13.46 delay 1:00 @08:44:13.49 Handoff.select :blocked @08:44:13.66 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @08:44:36.28 Handoff.select :waste @08:44:36.43 0.4psi at evacEnd @08:44:36.92 Air.seek :fullVent @08:44:38.29 Archive.in :fast @08:44:38.38 Archive.seek :topVent @08:44:45.07 Seawater evacuated, Pressure above/below filter released. @08:44:45.19 Lysis1.in :slow @08:44:45.35 Lysis1.seek 1050uL @08:45:14.99 delay 5 seconds @08:45:20.02 Handoff.select :blocked @08:45:20.30 Lysis1.seek 450uL @08:46:19.46 delay 3 seconds @08:46:22.56 Air.in mix with 1.57127 parts Lysis1 @08:46:23.22 Air.seek :evac @08:46:23.33 Lysis1.seek 300uL @08:46:38.28 Lysis1.seek :empty @08:47:07.98 Lysis buffer delivered @28.0psi @08:47:07.99 Polling Can every 2:00 @08:47:08.58 PuckHeater.configure :default #ambient 20.7C @08:47:08.66 PuckHeater.seek 79.8C @08:48:02.30 PuckHeater.hold 2:00,79.8C @08:50:02.21 Polling Can every 2:00 @08:50:02.78 PuckHeater.configure :default #ambient 20.8C @08:50:02.87 PuckHeater.hold 30 seconds,78.7C @08:50:33.07 Polling Can every 2:00 @08:50:33.62 PuckHeater.configure :default #ambient 20.8C @08:50:33.72 PuckHeater.hold 30 seconds,78.2C @08:51:03.91 Polling Can every 2:00 @08:51:04.46 PuckHeater.configure :default #ambient 20.8C @08:51:04.56 PuckHeater.hold 10:00,77.7C @08:51:34.03 -> Syringe.psi 34.2874680477775 @08:57:09.34 -> Syringe.psi 34.457728932597 @08:58:22.31 -> Syringe.psi 34.4198931804149 @09:01:03.82 Lysis complete. Syringe pressure @34.3psi @09:01:03.86 Archive syringe pumping up to 5 strokes to reach 40psi @09:01:03.89 Archive stroke #1 of 5 @09:01:04.04 Archive.in :pressurize @09:01:04.11 Archive.seek :pressurized @09:01:16.58 33.9psi after pressurize @09:01:22.17 33.2psi after pressurize (stable) @09:01:22.19 Archive.in :compress @09:01:22.33 Archive.seek :empty @09:01:26.72 Reached 41.8 of 40.0psi after pumping 1486uL (0.86 strokes) @09:01:26.74 Archive.in :default @09:01:26.85 Archive.hold @09:01:26.94 delay 2 seconds @09:01:28.97 Archive.in :fast @09:01:29.06 Archive.seek :full @09:01:35.46 Lysis1.seek 16uL @09:01:37.16 delay 1 second @09:01:38.20 Archive.seek :full @09:01:38.32 Lysis1.seek :empty @09:01:40.07 Handoff.select :blocked @09:01:40.31 Initial air pressure @50.0psi @09:01:40.43 Lysis1.in :extract @09:01:40.51 Lysis1.seek 600uL,nil @09:01:42.40 Archive syringe pumping up to 6 strokes to reach 25psi @09:01:42.42 Archive stroke #1 of 6 @09:01:42.57 Archive.in :pressurize @09:01:42.68 Archive.seek :pressurized @09:01:55.16 17.8psi after pressurize @09:02:00.66 Archive.in :default @09:02:00.73 Archive.hold @09:02:00.97 Initial 600uL lysate @16.1psi @09:02:00.99 Handoff.select :blocked @09:02:01.14 Initial air pressure @17.2psi @09:02:01.26 Lysis1.seek 825uL,nil @09:02:02.01 Archive syringe pumping up to 6 strokes to reach 17psi @09:02:02.02 Archive stroke #1 of 6 @09:02:02.18 Archive.in :compress @09:02:02.31 Archive.seek :empty @09:02:09.18 Archive.in :default @09:02:09.25 Archive.hold @09:02:09.53 Recovered 825uL lysate @15.3psi @09:02:09.56 Air.in :halfSpeed @09:02:09.67 Air.seek :fullVent @09:02:11.71 Puck Top Vented, bottom @16.9psi @09:02:11.72 Handoff.select :waste @09:02:11.89 delay 5 seconds @09:02:16.97 Handoff open to waste, bottom @-0.0psi @09:02:16.99 Air.in :default @09:02:17.11 Air.seek :evac @09:02:18.73 Handoff.select :close @09:02:18.92 Gate.power :heat,:OFF @09:02:18.94 Gate.power :cartridge,:OFF @09:02:19.08 PinPort.in :unlocking @09:02:19.17 PinPort.seek :clear @09:02:19.68 Carousel.in :default @09:02:19.83 Carousel.home.seek 8 @09:02:20.18 Plunge.seek :locked @09:02:21.14 Archive.in :unlocking @09:02:21.28 Archive.seek :clear @09:02:21.41 Air.in :unlocking @09:02:21.59 Air.seek :clear @09:02:21.69 Lysis1.in :unlocking @09:02:21.87 Lysis1.seek :clear @09:02:22.01 Lysis2.in :unlocking @09:02:22.11 Lysis2.seek :clear @09:02:26.73 Gate.power :analytic1,:OFF @09:02:26.77 Gate.power :motor,:OFF @09:02:26.80 Gate.power :core,:OFF @09:02:26.84 Gate.power :main,:OFF @09:02:26.89 Safely stopped and ready to power off @09:02:33.14 -> Cartridge.unlock @09:02:33.22 BusOff in preston -- core bus was not powered while reading Handoff Pin Port (PP)'s status @09:02:40.22 -> ESP.ready! @09:02:40.31 Gate.power :sampler,:OFF,limit to 3500mA @09:02:40.33 Gate.power :skimmer,:OFF,limit to 3500mA @09:02:40.37 Gate.power :main,:ON,limit to 5000mA @09:02:40.40 Gate.power :analytic1,:ON,limit to 4000mA @09:02:40.52 Powering core bus @09:02:43.30 Plunge.configure :default @09:02:43.42 SamplePump.configure :default @09:02:43.54 Toroid.configure :default @09:02:43.65 Carousel.configure :default @09:02:43.77 Abs.configure :default @09:02:43.87 PhaseSeparator.configure :default @09:02:43.98 Archive.configure :default @09:02:44.10 Air.configure :default @09:02:44.17 Lysis1.configure :default @09:02:44.54 Lysis2.configure :default @09:02:44.65 PinPort.configure :default @09:02:44.74 All elf controllers running firmware version 4.92 @09:02:44.77 Gate.power :motor,:ON,limit to 5000mA @09:02:47.78 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @09:02:47.81 AnalyticModule::Unknown in preston -- SPR @09:02:48.46 Carousel.home.jog 0 @09:02:48.51 Plunge.home.jog 0 @09:02:48.55 Archive.home.jog 0 @09:02:48.64 Toroid.home.jog 0 @09:02:49.99 Lysis2.home.jog 0 @09:02:50.03 Air.home.jog 0 @09:02:50.09 Lysis1.home.jog 0 @09:02:50.15 PinPort.home.jog 0 @09:02:50.91 Toroid.in :homing @09:02:50.98 Plunge.in :homing @09:02:51.02 Archive.in :homing @09:02:51.18 Toroid.jog 620 @09:02:51.22 Plunge.jog 1622 @09:02:51.26 Archive.jog 1403 @09:02:51.62 Lysis2.in :homing @09:02:51.67 Lysis1.in :homing @09:02:51.70 PinPort.in :homing @09:02:51.73 Air.in :homing @09:02:51.97 Lysis2.jog 1405 @09:02:52.02 Lysis1.jog 1399 @09:02:52.09 PinPort.jog -108 @09:02:52.27 Air.jog 1371 @09:02:52.57 PinPort.setRawPosition! 0 @09:02:52.66 PinPort.seek :home @09:02:52.81 PinPort.in :default @09:02:52.84 Archive.setRawPosition! 50 @09:02:52.87 Plunge.setRawPosition! 50 @09:02:53.08 Archive.seek :home @09:02:53.11 Plunge.seek :home @09:02:53.33 Lysis2.setRawPosition! 50 @09:02:53.41 Lysis2.seek :home @09:02:53.61 Air.setRawPosition! 50 @09:02:53.66 Archive.in :default @09:02:53.70 Plunge.in :default @09:02:53.75 Lysis1.setRawPosition! 50 @09:02:53.79 Toroid.setRawPosition! 0 @09:02:54.02 Air.seek :home @09:02:54.25 Toroid.seek :home @09:02:54.35 Lysis1.seek :home @09:02:54.55 Lysis2.in :default @09:02:54.59 Air.in :default @09:02:54.62 Toroid.in :default @09:02:54.87 Lysis1.in :default @09:02:55.23 Toroid.seek :clear @09:02:56.96 Carousel.in :default @09:02:57.11 Carousel.home.seek 8 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 8 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @09:03:56.27 -> to 8 @09:03:56.58 Carousel.seek 8 @09:03:56.84 Lysis1.in :fast @09:03:56.93 Lysis1.seek :locked @09:03:59.99 Plunge.seek :locked @09:04:00.32 Air.in :fast @09:04:00.41 Air.seek :locked @09:04:01.43 Carousel.reconfigure :locked @09:04:01.60 Powering cartridge bus @09:04:04.29 Gate.setIR 1750,5000,0,6 @09:04:04.44 Gate.setIR 0,5000,0,6 @09:04:04.54 PuckHeater.configure :default #ambient 20.8C @09:04:04.64 Gate.power :heat,:ON,limit to 5000mA @09:04:05.33 Mounted lysis Cartridge board#Q05092 @09:04:05.37 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @09:04:28.50 -> PhytoSampler.prepareLGcart 0,0 @09:04:28.53 Handoff.select :waste @09:04:28.73 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:04:35.36 Syringe tare is 51% of full scale at 21C (drifted -0%) @09:04:35.39 PinPort.in :locking @09:04:35.50 PinPort.seek :locked @09:04:36.02 Archive.in :fast @09:04:36.13 Lysis2.in :fast @09:04:36.17 Lysis1.seek :empty @09:04:36.35 Archive.seek :full @09:04:36.41 Lysis2.seek :empty Archive Syringe (ArS) full @09:04:53.47 -> Air.evac @09:04:53.53 Air.in :default @09:04:53.61 Air.seek :evac Air Syringe (AirS) at evac @09:05:57.10 -> Handoff.blocked @09:05:57.12 Handoff.select :blocked Handoff Valve::Manifold selects blocked @09:06:36.16 -> ArS.pumpTo 50; 10.times {puts Syringe.psi; Delay.sleep 60}; Syringe.psi @09:06:36.20 Archive syringe pumping up to 8 strokes to reach 50psi @09:06:36.26 Archive stroke #1 of 8 @09:06:36.40 Archive.in :pressurize @09:06:36.49 Archive.seek :pressurized @09:06:48.94 3.4psi after pressurize @09:07:03.53 9.3psi after pressurize (stable) @09:07:03.56 Archive.in :compress @09:07:03.67 Archive.seek :empty @09:07:19.39 15.8psi after compress @09:07:29.98 15.5psi after compress (stable) @09:07:30.01 Archive.in :gulp @09:07:30.12 Archive.seek :topVent @09:07:36.44 16.6psi after gulp @09:07:42.53 15.8psi after gulp (stable) @09:07:42.55 Archive stroke #2 of 8 @09:07:42.69 Archive.in :pressurize @09:07:42.76 Archive.seek :pressurized @09:07:55.21 16.3psi after pressurize @09:08:07.79 21.3psi after pressurize (stable) @09:08:07.82 Archive.in :compress @09:08:07.92 Archive.seek :empty @09:08:23.65 32.1psi after compress @09:08:34.27 31.6psi after compress (stable) @09:08:34.30 Archive.in :gulp @09:08:34.41 Archive.seek :topVent @09:08:40.74 32.7psi after gulp @09:08:46.33 31.9psi after gulp (stable) @09:08:46.35 Archive stroke #3 of 8 @09:08:46.48 Archive.in :pressurize @09:08:46.57 Archive.seek :pressurized @09:08:59.03 32.1psi after pressurize @09:09:05.12 31.3psi after pressurize (stable) @09:09:05.15 Archive.in :compress @09:09:05.25 Archive.seek :empty @09:09:21.00 47.3psi after compress @09:09:31.59 46.7psi after compress (stable) @09:09:31.62 Archive.in :gulp @09:09:31.73 Archive.seek :topVent @09:09:38.07 48.1psi after gulp @09:09:44.16 47.3psi after gulp (stable) @09:09:44.17 Archive stroke #4 of 8 @09:09:44.35 Archive.in :pressurize @09:09:44.42 Archive.seek :pressurized @09:09:56.89 47.2psi after pressurize @09:10:02.97 46.3psi after pressurize (stable) @09:10:03.00 Archive.in :compress @09:10:03.11 Archive.seek :empty @09:10:08.51 Reached 50.1 of 50.0psi after pumping 6697uL (3.87 strokes) @09:10:08.53 Archive.in :default @09:10:08.63 Archive.hold 49.007940381128 @09:21:54.39 -> Handoff.waste @09:21:54.41 Handoff.select :waste Handoff Valve::Manifold selects waste @09:22:20.26 -> Air.fVent @09:22:20.29 Air.seek :fVent Air Syringe (AirS) at fVent @09:22:24.80 -> Syringe.psi 0.171443252075164 @09:22:28.83 -> Cartridge.unlock @09:22:28.88 Gate.power :heat,:OFF @09:22:28.91 Gate.power :cartridge,:OFF @09:22:29.04 PinPort.in :unlocking @09:22:29.13 PinPort.seek :clear @09:22:30.07 Carousel.in :default @09:22:30.38 Carousel.home.seek 8 @09:22:30.75 Archive.in :unlocking @09:22:30.93 Archive.seek :clear @09:22:31.03 Air.in :unlocking @09:22:31.21 Air.seek :clear @09:22:31.35 Lysis2.in :unlocking @09:22:31.46 Lysis2.seek :clear @09:22:36.03 Lysis1.in :unlocking @09:22:36.19 Lysis1.seek :clear @09:22:36.30 Plunge.in :unlocking @09:22:36.42 Plunge.seek :clear @09:22:52.30 -> SC.to 40 @09:22:52.32 Carousel.seek 40 Storage Carousel (SC) at 40 @09:26:46.14 -> SC.to 15 @09:26:46.20 Carousel.seek 15 Storage Carousel (SC) at 15 @09:27:35.12 -> slots 15,40 =>[lyseHold_phyto] {15 => nil, 40 => { Type: :lyseHold_phyto, State: :dry}} @09:27:48.66 -> slots [15,40] =>[lyseHold_phyto] {[40,15] => { Type: :lyseHold_phyto, State: :dry}} @09:28:02.37 -> fire lyseHold_phyto, lyseHold_phyto # @09:28:03.34 Toroid.seek :clear @09:28:03.55 Carousel.in :default @09:28:03.68 Carousel.home.seek 15 @09:28:04.00 Selecting Cartridge 40 @09:28:04.35 Carousel.seek 40 @09:28:09.80 Lysis1.in :fast @09:28:09.88 Lysis1.seek :locked @09:28:12.53 Plunge.in :default @09:28:12.63 Plunge.seek :locked @09:28:12.81 Air.in :fast @09:28:12.90 Air.seek :locked @09:28:13.89 Carousel.reconfigure :locked @09:28:14.08 Powering cartridge bus @09:28:16.73 Gate.setIR 1750,5000,0,6 @09:28:16.86 Gate.setIR 0,5000,0,6 @09:28:16.96 PuckHeater.configure :default #ambient 20.8C @09:28:17.06 Gate.power :heat,:ON,limit to 5000mA @09:28:18.15 Mounted lysis Cartridge board#Q05075 @09:28:18.35 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:28:18.40 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 40" @09:28:18.89 Preparing to filter cartridge 40 @09:28:19.22 Air.in :default @09:28:19.38 Air.seek :sealed @09:28:19.58 Priming sample loop w/30ml, bypass w/50ml @09:28:19.60 SamplePump.setPosition! 0ml @09:28:19.74 Bypass.open @09:28:21.49 Intake.open @09:28:21.93 upsync @09:28:22.35 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:28:24.98 Vacuum tare is 52% of full scale at 21C (drifted 1%) @09:28:25.17 Abs.in :default @09:28:25.26 Abs.hold @09:28:25.54 SamplePump.in :start @09:28:25.65 SamplePump.seek 50ml,3:50 @09:28:26.25 SamplePump.configure :prime @09:28:59.88 -> PhytoSampler.bypassVolume=10 10 @09:29:27.03 Abs.stop @09:29:27.13 SamplePump.setPosition! 0ml @09:29:27.21 Exhaust.open @09:29:28.60 Bypass.close @09:29:30.15 Abs.in :default @09:29:30.28 Abs.hold @09:29:30.62 SamplePump.in :start @09:29:30.73 SamplePump.seek 30ml,2:30 @09:29:31.31 SamplePump.configure :prime @09:30:07.71 Abs.stop @09:30:07.82 Bypass.open @09:30:08.51 Intake.close @09:30:09.27 Toroid.in :hold @09:30:09.81 Toroid.seek :crack @09:30:11.49 Toroid.seek :cartridge @09:30:12.63 Toroid.in :default @09:30:12.72 Toroid.seek :clear @09:30:14.80 Pressurizing cartridge to 26psi above 15.9psia @09:30:14.81 SamplePump.setPosition! 0ml @09:30:14.93 Intake.open @09:30:15.60 Bypass.close @09:30:16.27 Exhaust.close @09:30:17.06 Abs.in :default @09:30:17.14 Abs.hold @09:30:17.39 SamplePump.in :charge @09:30:17.49 SamplePump.seek 2.3ml,42 seconds @09:30:32.16 Abs.stop @09:30:32.30 Intake.close @09:30:33.07 Pressurized cartridge with 0.966ml to 26.1psi @09:30:33.09 Isolated @38.4psia (22.4psi) @09:30:33.11 Verifying cartridge holds at least 0.65..0.85 of 38.4psia @09:30:33.22 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:30:37.82 Cartridge pressure fell 2% to 37.4psia @09:30:37.95 Sampling 50.0ml @09:30:37.97 SamplePump.setPosition! 0ml @09:30:38.08 Exhaust.open @09:30:38.75 Intake.open @09:30:39.63 Abs.in :default @09:30:39.70 Abs.hold @09:30:40.06 SamplePump.in :start @09:30:40.17 SamplePump.seek 50ml,4:40 @09:30:40.75 SamplePump.configure :sample @09:30:43.16 Cartridge 40 filtering 2/50ml at 0.56ml/s with 30.9psi vacuum @09:31:43.22 Cartridge 40 filtering 41/50ml at 0.66ml/s with 13.4psi vacuum 10.3m deep (29.9psia) @09:31:56.14 Abs.stop @09:31:56.33 Intake.close @09:31:56.38 Exhaust.close @09:31:56.48 Handoff.select :close @09:31:56.79 Toroid.in :hold @09:31:57.48 Toroid.seek :bypass @09:31:59.17 Toroid.in :default @09:31:59.27 Toroid.seek :clear @09:32:00.92 Sampled 50.0ml @09:32:01.38 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 40" @09:32:01.91 Handoff.select :waste @09:32:02.10 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:32:04.15 upsync @09:32:08.73 Syringe tare is 51% of full scale at 21C (drifted -0%) @09:32:08.76 PinPort.in :locking @09:32:08.89 PinPort.seek :locked @09:32:09.43 Archive.in :fast @09:32:09.49 Lysis2.in :fast @09:32:09.56 Lysis1.seek 1350uL @09:32:09.77 Archive.seek :full @09:32:09.85 Lysis2.seek :empty @09:32:17.97 Plunge.seek :reagent @09:32:18.90 Evacuating puck with filter bubble point of 24psi @09:32:18.93 PinPort.seek :locked @09:32:19.08 Handoff.select :waste @09:32:19.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:32:25.74 Air syringe pumping up to 1 strokes to reach 8psi @09:32:25.75 Air stroke #1 of 1 @09:32:26.49 Air.in :pressurize @09:32:26.58 Air.seek :pressurized @09:32:32.27 1.1psi after pressurize @09:32:32.29 Air.in :compress @09:32:32.42 Air.seek :evac @09:32:35.48 1.9psi after compress @09:32:46.07 0.3psi after compress (stable) @09:32:46.09 Air.in :default @09:32:46.20 Air.hold @09:32:46.47 at 0.2psi Opened to waste @09:32:46.49 Handoff.select :blocked @09:32:46.69 Archive syringe pumping up to 8 strokes to reach 24psi @09:32:46.70 Archive stroke #1 of 8 @09:32:46.83 Archive.in :pressurize @09:32:46.92 Archive.seek :pressurized @09:32:59.38 1.2psi after pressurize @09:33:04.98 0.5psi after pressurize (stable) @09:33:05.00 Archive.in :compress @09:33:05.10 Archive.seek :empty @09:33:20.84 17.3psi after compress @09:33:31.43 16.1psi after compress (stable) @09:33:31.55 Archive.in :gulp @09:33:31.67 Archive.seek :topVent @09:33:38.02 17.6psi after gulp @09:33:43.61 16.8psi after gulp (stable) @09:33:43.63 Archive stroke #2 of 8 @09:33:43.82 Archive.in :pressurize @09:33:43.96 Archive.seek :pressurized @09:33:56.43 17.2psi after pressurize @09:34:02.02 16.5psi after pressurize (stable) @09:34:02.05 Archive.in :compress @09:34:02.15 Archive.seek :empty @09:34:10.69 Reached 24.2 of 24.0psi after pumping 3306uL (1.91 strokes) @09:34:10.71 Archive.in :default @09:34:10.83 Archive.hold @09:34:10.95 Handoff.select :waste @09:34:11.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:34:21.21 delay 1:00 @09:35:21.34 Handoff.select :blocked @09:35:21.51 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @09:35:44.10 Handoff.select :waste @09:35:44.39 0.4psi at evacEnd @09:35:44.41 Handoff.select :blocked @09:35:44.60 Archive.in :fast @09:35:44.69 Archive.seek :topVent @09:35:51.35 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @09:35:57.94 Stable @0.2psi after Handoff blocked @09:35:58.56 Archive syringe pumping up to 8 strokes to reach 24psi @09:35:58.60 Archive stroke #1 of 8 @09:35:58.74 Archive.in :pressurize @09:35:58.83 Archive.seek :pressurized @09:36:11.27 3.2psi after pressurize @09:36:11.30 Archive.in :compress @09:36:11.42 Archive.seek :empty @09:36:27.17 15.6psi after compress @09:36:37.76 14.5psi after compress (stable) @09:36:37.78 Archive.in :gulp @09:36:37.91 Archive.seek :topVent @09:36:44.29 15.7psi after gulp @09:36:49.88 14.9psi after gulp (stable) @09:36:49.89 Archive stroke #2 of 8 @09:36:50.02 Archive.in :pressurize @09:36:50.10 Archive.seek :pressurized @09:37:02.57 15.2psi after pressurize @09:37:02.60 Archive.in :compress @09:37:02.70 Archive.seek :empty @09:37:12.44 Reached 24.5 of 24.2psi after pumping 3331uL (1.93 strokes) @09:37:12.46 Archive.in :default @09:37:12.56 Archive.hold @09:37:12.66 Handoff.select :waste @09:37:12.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:37:19.91 delay 1:00 @09:38:18.25 -> Syringe.psi 0.00354710176708295 @09:38:20.01 Handoff.select :blocked @09:38:20.19 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @09:38:28.44 -> Syringe.psi 0.358257278474326 @09:38:36.69 -> Syringe.psi 0.405551968701957 @09:38:42.78 Handoff.select :waste @09:38:42.94 0.4psi at evacEnd @09:38:42.97 Air.seek :fullVent @09:38:44.34 Archive.in :fast @09:38:44.44 Archive.seek :topVent @09:38:51.17 Seawater evacuated, Pressure above/below filter released. @09:38:51.78 Lysis1.in :slow @09:38:51.98 Lysis1.seek 1050uL @09:39:21.61 delay 5 seconds @09:39:26.63 Handoff.select :blocked @09:39:26.86 Lysis1.seek 450uL @09:40:26.04 delay 3 seconds @09:40:29.13 Air.in mix with 1.57127 parts Lysis1 @09:40:29.80 Air.seek :evac @09:40:29.91 Lysis1.seek 300uL @09:40:44.87 Lysis1.seek :empty @09:41:14.59 Lysis buffer delivered @12.8psi @09:41:14.61 Polling Can every 2:00 @09:41:15.18 PuckHeater.configure :default #ambient 21.4C @09:41:15.27 PuckHeater.seek 79.8C @09:42:13.13 PuckHeater.hold 2:00,79.8C @09:43:38.72 -> Syringe.psi 13.4541570025056 @09:43:40.74 -> Syringe.psi 13.4021328432552 @09:43:42.79 -> Syringe.psi 13.326461338891 @09:44:12.65 Polling Can every 2:00 @09:44:13.20 PuckHeater.hold 30 seconds,78.7C @09:44:43.28 Polling Can every 2:00 @09:44:43.83 PuckHeater.configure :default #ambient 21.3C @09:44:43.93 PuckHeater.hold 30 seconds,78.2C @09:45:13.99 Polling Can every 2:00 @09:45:14.55 PuckHeater.configure :default #ambient 21.3C @09:45:14.63 PuckHeater.hold 10:00,77.7C @09:55:11.53 Lysis complete. Syringe pressure @14.1psi @09:55:11.56 Archive syringe pumping up to 5 strokes to reach 40psi @09:55:11.58 Archive stroke #1 of 5 @09:55:11.71 Archive.in :pressurize @09:55:11.79 Archive.seek :pressurized @09:55:24.30 14.6psi after pressurize @09:55:29.89 13.6psi after pressurize (stable) @09:55:29.92 Archive.in :compress @09:55:30.03 Archive.seek :empty @09:55:39.58 Reached 40.4 of 40.0psi after pumping 1597uL (0.92 strokes) @09:55:39.60 Archive.in :default @09:55:39.72 Archive.hold @09:55:39.83 delay 2 seconds @09:55:41.87 Archive.in :fast @09:55:41.95 Archive.seek :full @09:55:48.74 Lysis1.seek 16uL @09:55:50.45 delay 1 second @09:55:51.49 Archive.seek :full @09:55:51.55 Lysis1.seek :empty @09:55:53.34 Handoff.select :blocked @09:55:53.54 Initial air pressure @42.2psi @09:55:53.63 Lysis1.in :extract @09:55:53.79 Lysis1.seek 600uL,nil @09:55:59.10 Archive syringe pumping up to 6 strokes to reach 25psi @09:55:59.12 Archive stroke #1 of 6 @09:55:59.41 Archive.in :default @09:55:59.49 Archive.hold @09:55:59.68 Initial 600uL lysate @13.6psi @09:55:59.69 Handoff.select :blocked @09:55:59.84 Initial air pressure @13.6psi @09:55:59.96 Lysis1.seek 825uL,nil @09:56:00.09 Archive syringe pumping up to 6 strokes to reach 17psi @09:56:00.11 Archive stroke #1 of 6 @09:56:00.34 Archive.in :pressurize @09:56:00.45 Archive.seek :pressurized @09:56:13.02 14.1psi after pressurize @09:56:16.61 Reached 17.1 of 17.0psi after pumping 1397uL (0.81 strokes) @09:56:16.73 Archive.in :default @09:56:16.82 Archive.hold @09:56:17.10 Archive syringe pumping up to 6 strokes to reach 17psi @09:56:17.16 Archive stroke #1 of 6 @09:56:17.34 Archive.in :compress @09:56:17.43 Archive.seek :empty @09:56:18.95 Archive.in :default @09:56:19.03 Archive.hold @09:56:19.33 Recovered 825uL lysate @15.7psi @09:56:19.35 Air.in :halfSpeed @09:56:19.46 Air.seek :fullVent @09:56:21.49 Puck Top Vented, bottom @18.9psi @09:56:21.51 Handoff.select :waste @09:56:21.67 delay 5 seconds @09:56:26.75 Handoff open to waste, bottom @0.4psi @09:56:26.77 Air.in :default @09:56:26.88 Air.seek :evac @09:56:28.37 Selecting Cartridge 15 @09:56:28.57 Gate.power :heat,:OFF @09:56:28.60 Gate.power :cartridge,:OFF @09:56:28.74 PinPort.in :unlocking @09:56:28.83 PinPort.seek :clear @09:56:29.34 Carousel.in :default @09:56:29.50 Carousel.home.seek 40 @09:56:29.86 Archive.in :unlocking @09:56:30.04 Archive.seek :clear @09:56:30.14 Air.in :unlocking @09:56:30.36 Air.seek :clear @09:56:30.49 Lysis2.in :unlocking @09:56:30.60 Lysis2.seek :clear @09:56:35.17 Lysis1.in :unlocking @09:56:35.34 Lysis1.seek :clear @09:56:35.42 Plunge.in :unlocking @09:56:35.57 Plunge.seek :clear @09:56:38.29 Carousel.seek 15 @09:56:43.72 Lysis1.in :fast @09:56:43.81 Lysis1.seek :locked @09:56:46.46 Plunge.in :default @09:56:46.63 Plunge.seek :locked @09:56:46.74 Air.in :fast @09:56:46.82 Air.seek :locked @09:56:47.82 Carousel.reconfigure :locked @09:56:48.58 Powering cartridge bus @09:56:51.26 Gate.setIR 1750,5000,0,6 @09:56:51.39 Gate.setIR 0,5000,0,6 @09:56:51.48 PuckHeater.configure :default #ambient 21.3C @09:56:51.58 Gate.power :heat,:ON,limit to 5000mA @09:56:52.29 Mounted lysis Cartridge board#Q05092 @09:56:52.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:56:52.42 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling lyseHold_phyto cartridge in slot 15" @09:56:52.82 Preparing to filter cartridge 15 @09:56:53.20 Air.in :default @09:56:53.39 Air.seek :sealed @09:56:53.63 Priming sample loop w/30ml, bypass w/10ml @09:56:53.67 SamplePump.setPosition! 0ml @09:56:53.81 Bypass.open @09:56:55.37 upsync @09:56:55.41 Intake.open @09:56:56.28 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:56:58.90 Vacuum tare is 52% of full scale at 21C (drifted 1%) @09:56:59.10 Abs.in :default @09:56:59.19 Abs.hold @09:56:59.48 SamplePump.in :start @09:56:59.58 SamplePump.seek 10ml,1:10 @09:57:00.17 SamplePump.configure :prime @09:57:12.15 Abs.stop @09:57:12.38 SamplePump.setPosition! 0ml @09:57:12.47 Exhaust.open @09:57:13.83 Bypass.close @09:57:15.39 Abs.in :default @09:57:15.48 Abs.hold @09:57:15.83 SamplePump.in :start @09:57:16.53 SamplePump.seek 30ml,2:30 @09:57:16.63 SamplePump.configure :prime @09:57:53.33 Abs.stop @09:57:53.45 Bypass.open @09:57:54.12 Intake.close @09:57:54.87 Toroid.in :hold @09:57:54.96 Toroid.seek :crack @09:57:56.55 Toroid.seek :cartridge @09:57:57.70 Toroid.in :default @09:57:57.79 Toroid.seek :clear @09:57:59.85 Pressurizing cartridge to 26psi above 15.8psia @09:57:59.87 SamplePump.setPosition! 0ml @09:57:59.99 Intake.open @09:58:00.67 Bypass.close @09:58:01.31 Exhaust.close @09:58:02.12 Abs.in :default @09:58:02.21 Abs.hold @09:58:02.51 SamplePump.in :charge @09:58:02.61 SamplePump.seek 2.3ml,42 seconds @09:58:16.68 Abs.stop @09:58:16.78 Intake.close @09:58:17.54 Pressurized cartridge with 0.891ml to 28.7psi @09:58:17.56 Isolated @37.9psia (22.1psi) @09:58:17.58 Verifying cartridge holds at least 0.65..0.85 of 37.9psia @09:58:17.68 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:58:22.31 Cartridge pressure fell 3% to 37.0psia @09:58:22.44 Sampling 50.0ml @09:58:22.46 SamplePump.setPosition! 0ml @09:58:22.57 Exhaust.open @09:58:23.26 Intake.open @09:58:24.14 Abs.in :default @09:58:24.26 Abs.hold @09:58:24.61 SamplePump.in :start @09:58:24.71 SamplePump.seek 50ml,4:40 @09:58:25.30 SamplePump.configure :sample @09:58:27.70 Cartridge 15 filtering 2/50ml at 0.66ml/s with 19.2psi vacuum @09:59:28.22 Cartridge 15 filtering 40/50ml at 0.64ml/s with 21.3psi vacuum @09:59:42.85 Abs.stop @09:59:43.04 Intake.close @09:59:43.10 Exhaust.close @09:59:43.18 Handoff.select :close @09:59:43.55 Toroid.in :hold @09:59:43.65 Toroid.seek :bypass @09:59:45.32 Toroid.in :default @09:59:45.42 Toroid.seek :clear @09:59:47.08 Sampled 50.0ml @09:59:47.50 GoodNews.email "After filtering 50.0ml",Subject: "Processing lyseHold_phyto cartridge in slot 15" @09:59:48.10 Handoff.select :waste @09:59:48.31 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:59:50.14 upsync @09:59:54.94 Syringe tare is 51% of full scale at 21C (drifted 0%) @09:59:54.97 PinPort.in :locking @09:59:55.08 PinPort.seek :locked @09:59:55.61 Archive.in :fast @09:59:55.67 Lysis2.in :fast @09:59:55.77 Lysis1.seek 1350uL @09:59:55.97 Lysis2.seek :empty @09:59:56.04 Archive.seek :full @10:00:04.12 Plunge.seek :reagent @10:00:05.06 Evacuating puck with filter bubble point of 24psi @10:00:05.09 PinPort.seek :locked @10:00:05.24 Handoff.select :waste @10:00:05.27 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:00:15.50 Air syringe pumping up to 1 strokes to reach 8psi @10:00:15.52 Air stroke #1 of 1 @10:00:15.66 Air.in :pressurize @10:00:15.74 Air.seek :pressurized @10:00:21.40 0.8psi after pressurize @10:00:21.43 Air.in :compress @10:00:21.55 Air.seek :evac @10:00:24.59 1.7psi after compress @10:00:35.18 -0.3psi after compress (stable) @10:00:35.19 Air.in :default @10:00:35.31 Air.hold @10:00:35.55 at -0.2psi Opened to waste @10:00:35.57 Handoff.select :blocked @10:00:35.75 Archive syringe pumping up to 8 strokes to reach 24psi @10:00:35.77 Archive stroke #1 of 8 @10:00:35.90 Archive.in :pressurize @10:00:35.99 Archive.seek :pressurized @10:00:48.44 0.8psi after pressurize @10:01:06.03 7.9psi after pressurize (stable) @10:01:06.06 Archive.in :compress @10:01:06.16 Archive.seek :empty @10:01:21.93 17.3psi after compress @10:01:32.52 16.3psi after compress (stable) @10:01:32.55 Archive.in :gulp @10:01:32.77 Archive.seek :topVent @10:01:39.11 17.2psi after gulp @10:01:44.71 16.4psi after gulp (stable) @10:01:44.72 Archive stroke #2 of 8 @10:01:44.86 Archive.in :pressurize @10:01:44.94 Archive.seek :pressurized @10:01:57.83 17.0psi after pressurize @10:02:03.52 16.2psi after pressurize (stable) @10:02:03.55 Archive.in :compress @10:02:03.67 Archive.seek :empty @10:02:10.72 Reached 24.0 of 24.0psi after pumping 3273uL (1.89 strokes) @10:02:10.74 Archive.in :default @10:02:10.86 Archive.hold @10:02:10.96 Handoff.select :waste @10:02:11.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:02:19.72 delay 1:00 @10:03:19.75 Handoff.select :blocked @10:03:19.92 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @10:03:42.50 Handoff.select :waste @10:03:42.80 0.3psi at evacEnd @10:03:42.81 Handoff.select :blocked @10:03:43.01 Archive.in :fast @10:03:43.10 Archive.seek :topVent @10:03:49.65 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @10:03:56.27 Stable @-0.0psi after Handoff blocked @10:03:56.30 Archive syringe pumping up to 8 strokes to reach 24psi @10:03:56.34 Archive stroke #1 of 8 @10:03:56.49 Archive.in :pressurize @10:03:56.58 Archive.seek :pressurized @10:04:09.04 4.7psi after pressurize @10:04:09.07 Archive.in :compress @10:04:09.18 Archive.seek :empty @10:04:24.92 16.6psi after compress @10:04:35.52 15.3psi after compress (stable) @10:04:35.65 Archive.in :gulp @10:04:35.77 Archive.seek :topVent @10:04:42.12 16.3psi after gulp @10:04:47.72 15.6psi after gulp (stable) @10:04:47.73 Archive stroke #2 of 8 @10:04:47.88 Archive.in :pressurize @10:04:47.97 Archive.seek :pressurized @10:05:00.43 16.0psi after pressurize @10:05:00.45 Archive.in :compress @10:05:00.55 Archive.seek :empty @10:05:08.38 Reached 24.2 of 24.0psi after pumping 3290uL (1.90 strokes) @10:05:08.40 Archive.in :default @10:05:08.50 Archive.hold @10:05:08.63 Handoff.select :waste @10:05:08.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:05:15.89 delay 1:00 @10:06:15.92 Handoff.select :blocked @10:06:16.09 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @10:06:38.68 Handoff.select :waste @10:06:38.90 0.1psi at evacEnd @10:06:38.93 Air.seek :fullVent @10:06:40.29 Archive.in :fast @10:06:40.38 Archive.seek :topVent @10:06:46.99 Seawater evacuated, Pressure above/below filter released. @10:06:47.11 Lysis1.in :slow @10:06:47.19 Lysis1.seek 1050uL @10:07:16.84 delay 5 seconds @10:07:21.88 Handoff.select :blocked @10:07:22.12 Lysis1.seek 450uL @10:08:21.30 delay 3 seconds @10:08:24.41 Air.in mix with 1.57127 parts Lysis1 @10:08:25.18 Air.seek :evac @10:08:25.28 Lysis1.seek 300uL @10:08:40.27 Lysis1.seek :empty @10:09:09.99 Lysis buffer delivered @17.4psi @10:09:10.00 Polling Can every 2:00 @10:09:10.58 PuckHeater.configure :default #ambient 21.5C @10:09:10.66 PuckHeater.seek 79.8C @10:10:02.07 PuckHeater.hold 2:00,79.8C @10:10:29.09 -> Syringe.psi 19.3967348296076 @10:12:02.07 Polling Can every 2:00 @10:12:02.62 PuckHeater.configure :default #ambient 21.5C @10:12:02.81 PuckHeater.hold 30 seconds,78.7C @10:12:33.01 Polling Can every 2:00 @10:12:33.55 PuckHeater.configure :default #ambient 21.4C @10:12:33.64 PuckHeater.hold 30 seconds,78.2C @10:13:03.82 Polling Can every 2:00 @10:13:04.37 PuckHeater.configure :default #ambient 21.5C @10:13:05.05 PuckHeater.hold 10:00,77.7C @10:23:04.30 Lysis complete. Syringe pressure @20.7psi @10:23:04.33 Archive syringe pumping up to 5 strokes to reach 40psi @10:23:04.36 Archive stroke #1 of 5 @10:23:04.49 Archive.in :pressurize @10:23:04.58 Archive.seek :pressurized @10:23:17.04 20.9psi after pressurize @10:23:22.64 20.3psi after pressurize (stable) @10:23:22.66 Archive.in :compress @10:23:22.78 Archive.seek :empty @10:23:28.52 Reached 40.3 of 40.0psi after pumping 1516uL (0.88 strokes) @10:23:28.54 Archive.in :default @10:23:28.64 Archive.hold @10:23:28.75 delay 2 seconds @10:23:30.78 Archive.in :fast @10:23:30.86 Archive.seek :full @10:23:37.37 Lysis1.seek 16uL @10:23:39.07 delay 1 second @10:23:40.12 Archive.seek :full @10:23:40.19 Lysis1.seek :empty @10:23:42.01 Handoff.select :blocked @10:23:42.20 Initial air pressure @42.4psi @10:23:42.37 Lysis1.in :extract @10:23:42.48 Lysis1.seek 600uL,nil @10:23:46.19 Archive syringe pumping up to 6 strokes to reach 25psi @10:23:46.21 Archive stroke #1 of 6 @10:23:46.43 Archive.in :pressurize @10:23:46.54 Archive.seek :pressurized @10:23:58.99 12.8psi after pressurize @10:24:01.06 Archive.in :default @10:24:01.13 Archive.hold @10:24:01.33 Initial 600uL lysate @16.9psi @10:24:01.35 Handoff.select :blocked @10:24:01.48 Initial air pressure @17.0psi @10:24:01.60 Lysis1.seek 825uL,nil @10:24:03.49 Archive syringe pumping up to 6 strokes to reach 17psi @10:24:03.51 Archive stroke #1 of 6 @10:24:03.66 Archive.in :compress @10:24:03.78 Archive.seek :empty @10:24:04.38 Archive.in :default @10:24:04.46 Archive.hold @10:24:04.76 Recovered 825uL lysate @13.9psi @10:24:04.78 Air.in :halfSpeed @10:24:04.89 Air.seek :fullVent @10:24:07.51 Puck Top Vented, bottom @1.6psi @10:24:07.55 Handoff.select :waste @10:24:07.74 delay 5 seconds @10:24:12.82 Handoff open to waste, bottom @-0.1psi @10:24:12.85 Air.in :default @10:24:12.96 Air.seek :evac @10:24:14.51 Handoff.select :close @10:24:14.69 Gate.power :heat,:OFF @10:24:14.71 Gate.power :cartridge,:OFF @10:24:14.84 PinPort.in :unlocking @10:24:14.92 PinPort.seek :clear @10:24:15.44 Carousel.in :default @10:24:15.58 Carousel.home.seek 15 @10:24:15.93 Plunge.seek :locked @10:24:16.86 Archive.in :unlocking @10:24:17.01 Archive.seek :clear @10:24:17.19 Air.in :unlocking @10:24:17.34 Air.seek :clear @10:24:17.47 Lysis1.in :unlocking @10:24:17.62 Lysis1.seek :clear @10:24:17.76 Lysis2.in :unlocking @10:24:17.87 Lysis2.seek :clear @10:24:22.51 Gate.power :analytic1,:OFF @10:24:22.53 Gate.power :motor,:OFF @10:24:22.59 Gate.power :core,:OFF @10:24:22.61 Gate.power :main,:OFF @10:24:22.65 Safely stopped and ready to power off @10:28:04.42 -> ESP.ready! @10:28:04.49 Gate.power :sampler,:OFF,limit to 3500mA @10:28:04.53 Gate.power :skimmer,:OFF,limit to 3500mA @10:28:04.56 Gate.power :main,:ON,limit to 5000mA @10:28:04.61 Gate.power :analytic1,:ON,limit to 4000mA @10:28:04.73 Powering core bus @10:28:07.52 Plunge.configure :default @10:28:07.63 SamplePump.configure :default @10:28:07.75 Toroid.configure :default @10:28:07.87 Carousel.configure :default @10:28:07.97 Abs.configure :default @10:28:08.08 PhaseSeparator.configure :default @10:28:08.19 Archive.configure :default @10:28:08.34 Air.configure :default @10:28:08.42 Lysis1.configure :default @10:28:08.77 Lysis2.configure :default @10:28:08.88 PinPort.configure :default @10:28:08.97 All elf controllers running firmware version 4.92 @10:28:09.00 Gate.power :motor,:ON,limit to 5000mA @10:28:11.98 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:28:12.01 AnalyticModule::Unknown in preston -- SPR @10:28:12.32 Plunge.home.jog 0 @10:28:13.05 PinPort.home.jog 0 @10:28:13.12 Lysis2.home.jog 0 @10:28:13.15 Air.home.jog 0 @10:28:13.20 Carousel.home.jog 0 @10:28:13.25 Lysis1.home.jog 0 @10:28:13.34 Toroid.home.jog 0 @10:28:13.38 Archive.home.jog 0 @10:28:14.90 Plunge.in :homing @10:28:15.08 Plunge.jog 1608 @10:28:15.36 PinPort.in :homing @10:28:15.40 Lysis2.in :homing @10:28:15.45 Air.in :homing @10:28:15.50 Toroid.in :homing @10:28:15.54 Lysis1.in :homing @10:28:15.64 Archive.in :homing @10:28:15.81 PinPort.jog -109 @10:28:15.88 Lysis2.jog 1406 @10:28:15.95 Air.jog 1373 @10:28:16.09 Toroid.jog 623 @10:28:16.12 Lysis1.jog 1395 @10:28:16.16 Archive.jog 1405 @10:28:16.65 Plunge.setRawPosition! 50 @10:28:16.68 PinPort.setRawPosition! 0 @10:28:16.84 PinPort.seek :home @10:28:16.88 Plunge.seek :home @10:28:17.12 PinPort.in :default @10:28:17.29 Plunge.in :default @10:28:17.51 Lysis2.setRawPosition! 50 @10:28:17.62 Air.setRawPosition! 50 @10:28:17.66 Lysis2.seek :home @10:28:17.79 Air.seek :home @10:28:17.95 Archive.setRawPosition! 50 @10:28:17.98 Lysis1.setRawPosition! 50 @10:28:18.13 Lysis1.seek :home @10:28:18.16 Archive.seek :home @10:28:18.42 Air.in :default @10:28:18.45 Lysis2.in :default @10:28:18.68 Lysis1.in :default @10:28:18.72 Archive.in :default @10:28:18.76 Toroid.setRawPosition! 0 @10:28:18.97 Toroid.seek :home @10:28:19.10 Toroid.in :default @10:28:19.24 Toroid.seek :clear @10:28:20.99 Carousel.in :default @10:28:21.13 Carousel.home.seek 15 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 15 Abs = Absolute Pressure (Leak) Sensor @20psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:28:37.27 -> Handoff.waste @10:28:37.29 Handoff.select :waste Handoff Valve::Manifold selects waste @10:48:28.02 -> BACsampler.prime 500, 100 @10:48:28.13 Priming sample loop w/500ml, bypass w/100ml @10:48:28.15 SamplePump.setPosition! 0ml @10:48:28.28 Bypass.open @10:48:29.70 Intake.open @10:48:31.00 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:48:33.62 Vacuum tare is 52% of full scale at 21C (drifted 1%) @10:48:33.80 Abs.in :default @10:48:33.87 Abs.hold @10:48:33.98 PhaseSeparator.in :full @10:48:34.07 PhaseSeparator.hold @10:48:34.37 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @10:48:40.96 SamplePump.stop @10:48:41.06 Intake.close @10:48:41.14 Bypass.close @10:48:41.25 Handoff.select :close @10:48:42.01 Abs.stop @10:48:42.10 PhaseSeparator.stop @10:48:42.21 Scale::Skew::CannotTare in preston -- Intake Head Pressure sensor @31.6% scale out of usable range @10:49:00.78 -> PhytoSampler.prime 500, 100 @10:49:00.90 Priming sample loop w/500ml, bypass w/100ml @10:49:00.92 SamplePump.setPosition! 0ml @10:49:01.02 Bypass.open @10:49:02.47 Intake.open @10:49:03.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:49:05.85 Vacuum tare is 52% of full scale at 21C (drifted 1%) @10:49:06.02 Abs.in :default @10:49:06.11 Abs.hold @10:49:06.41 SamplePump.in :start @10:49:06.50 SamplePump.seek 100ml,7:10 @10:49:07.09 SamplePump.configure :prime @10:51:08.91 Abs.stop @10:51:09.00 SamplePump.setPosition! 0ml @10:51:09.59 Exhaust.open @10:51:11.00 Bypass.close @10:51:12.51 Abs.in :default @10:51:12.58 Abs.hold @10:51:12.91 SamplePump.in :start @10:51:13.00 SamplePump.seek 500ml,33:50 @10:51:13.60 SamplePump.configure :prime @11:01:23.39 Abs.stop PhytoSampler @11:50:02.11 -> SC.coast @11:50:02.13 Carousel.coast Storage Carousel (SC) at 15 @12:23:21.82 -> require 'vacuumCart' true @12:32:38.60 -> cleanToroid @12:32:38.63 Cleans Dry SC positions in slots, begin in 10% Bleach @12:32:39.43 Toroid.seek :clear @12:32:39.65 Carousel.in :default @12:32:39.79 Carousel.home.seek 15 @12:32:41.97 Waiting for preston to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @12:32:45.52 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @12:32:45.65 Priming sample loop w/100ml, bypass w/50ml @12:32:45.67 SamplePump.setPosition! 0ml @12:32:45.76 Exhaust.close @12:32:46.41 Bypass.open @12:32:48.03 Abs.in :default @12:32:48.12 Abs.hold @12:32:48.25 PhaseSeparator.hold @12:32:48.53 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:32:55.59 SamplePump.stop @12:32:55.78 Intake.close @12:32:55.90 Bypass.close @12:32:56.70 Abs.stop @12:32:56.78 PhaseSeparator.stop @12:32:56.89 Scale::Skew::CannotTare in preston -- Intake Head Pressure sensor @31.6% scale out of usable range @12:34:17.82 -> CleanSampler.source=nil @12:34:25.14 -> cleanToroid @12:34:25.17 Cleans Dry SC positions in slots, begin in 10% Bleach @12:34:25.93 Toroid.seek :clear @12:34:26.14 Carousel.in :default @12:34:26.32 Carousel.home.seek 15 @12:34:26.61 Waiting for preston to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @12:34:40.63 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @12:34:40.74 Priming sample loop w/100ml, bypass w/50ml @12:34:40.76 SamplePump.setPosition! 0ml @12:34:40.86 Bypass.open @12:34:42.41 Intake.open @12:34:43.94 Abs.hold @12:34:44.06 PhaseSeparator.hold @12:34:44.36 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:34:50.96 SamplePump.stop @12:34:51.05 Intake.close @12:34:51.16 Bypass.close @12:34:51.95 Abs.stop @12:34:52.03 PhaseSeparator.stop @12:34:52.14 Scale::Skew::CannotTare in preston -- Intake Head Pressure sensor @31.7% scale out of usable range @12:37:10.90 -> CleanSampler.source @12:37:20.72 -> CleanSampler.source.nil @12:37:20.74 NoMethodError in preston -- undefined method `nil' for nil:NilClass @12:37:31.22 -> CleanSampler.source=nil @12:37:39.60 -> CleanSampler.prime @12:37:39.72 Priming sample loop w/100ml, bypass w/10ml @12:37:39.74 SamplePump.setPosition! 0ml @12:37:39.84 Bypass.open @12:37:41.38 Intake.open @12:37:42.89 Abs.in :default @12:37:42.96 Abs.hold @12:37:43.24 SamplePump.in :start @12:37:43.34 SamplePump.seek 10ml,1:10 @12:37:43.93 SamplePump.configure :prime @12:37:45.63 SamplePump.hold @12:37:45.88 Intake.close @12:37:45.98 Bypass.close @12:37:46.80 Abs.stop @12:37:46.91 UserAbort in preston -- Sample Pump (SP) seek to 10ml Aborted! @12:38:02.41 -> BACsampler.source=nil @12:38:11.97 -> cleanToroid @12:38:11.99 Cleans Dry SC positions in slots, begin in 10% Bleach @12:38:12.85 Toroid.seek :clear @12:38:13.07 Carousel.in :default @12:38:13.22 Carousel.home.seek 15 @12:38:13.50 Waiting for preston to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @12:38:13.55 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @12:38:13.67 Priming sample loop w/100ml, bypass w/50ml @12:38:13.68 SamplePump.setPosition! 0ml @12:38:13.78 Bypass.open @12:38:15.35 Intake.open @12:38:16.86 Abs.in :default @12:38:16.94 Abs.hold @12:38:17.21 SamplePump.in :start @12:38:17.30 SamplePump.seek 50ml,3:50 @12:38:17.90 SamplePump.configure :prime @12:38:21.98 SamplePump.hold @12:38:22.21 Intake.close @12:38:22.35 Bypass.close @12:38:23.17 Abs.stop @12:38:23.86 UserAbort in preston -- Sample Pump (SP) seek to 50ml Aborted! @12:38:29.95 -> slots 1..60=>nil {60..1 => nil} @12:38:35.93 -> slots 1..60=>:dry {60..1 => { Type: :unknown, State: :dry}} @12:38:39.25 -> cleanToroid @12:38:39.28 Cleans Dry SC positions in slots, begin in 10% Bleach @12:38:40.12 Toroid.seek :clear @12:38:40.39 Carousel.in :default @12:38:40.54 Carousel.home.seek 15 @12:38:40.82 Waiting for preston to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @12:38:43.09 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @12:38:43.21 Priming sample loop w/100ml, bypass w/50ml @12:38:43.23 SamplePump.setPosition! 0ml @12:38:43.33 Bypass.open @12:38:44.86 Intake.open @12:38:46.41 Abs.in :default @12:38:46.48 Abs.hold @12:38:46.75 SamplePump.in :start @12:38:46.84 SamplePump.seek 50ml,3:50 @12:38:47.44 SamplePump.configure :prime @12:39:48.27 Abs.stop @12:39:48.36 SamplePump.setPosition! 0ml @12:39:48.45 Exhaust.open @12:39:49.79 Bypass.close @12:39:51.30 Abs.in :default @12:39:51.40 Abs.hold @12:39:51.72 SamplePump.in :start @12:39:51.82 SamplePump.seek 100ml,7:10 @12:39:52.41 SamplePump.configure :prime @12:41:54.19 Abs.stop @12:41:54.30 Opening all dry Cartridges @12:41:54.37 Ensuring (60) cartridge(s) open @12:41:54.67 Carousel.seek 1 @12:41:57.76 Toroid.in :hold @12:41:57.84 Toroid.seek :cartridge @12:41:59.71 Toroid.in :default @12:41:59.80 Toroid.seek :clear @12:42:01.90 Carousel.seek 2 @12:42:02.34 Toroid.in :hold @12:42:02.43 Toroid.seek :cartridge @12:42:04.31 Toroid.in :default @12:42:04.49 Toroid.seek :clear @12:42:06.61 Carousel.seek 3 @12:42:07.04 Toroid.in :hold @12:42:07.13 Toroid.seek :cartridge @12:42:08.99 Toroid.in :default @12:42:09.07 Toroid.seek :clear @12:42:11.15 Carousel.seek 4 @12:42:11.59 Toroid.in :hold @12:42:11.67 Toroid.seek :cartridge @12:42:13.53 Toroid.in :default @12:42:13.62 Toroid.seek :clear @12:42:15.71 Carousel.seek 5 @12:42:16.14 Toroid.in :hold @12:42:16.25 Toroid.seek :cartridge @12:42:18.13 Toroid.in :default @12:42:18.24 Toroid.seek :clear @12:42:20.39 Carousel.seek 6 @12:42:20.81 Toroid.in :hold @12:42:20.89 Toroid.seek :cartridge @12:42:22.76 Toroid.in :default @12:42:22.84 Toroid.seek :clear @12:42:24.94 Carousel.seek 7 @12:42:25.37 Toroid.in :hold @12:42:25.47 Toroid.seek :cartridge @12:42:27.33 Toroid.in :default @12:42:27.42 Toroid.seek :clear @12:42:29.54 Carousel.seek 8 @12:42:29.97 Toroid.in :hold @12:42:30.06 Toroid.seek :cartridge @12:42:31.93 Toroid.in :default @12:42:32.01 Toroid.seek :clear @12:42:34.64 Carousel.seek 9 @12:42:35.17 Toroid.in :hold @12:42:35.26 Toroid.seek :cartridge @12:42:37.13 Toroid.in :default @12:42:37.21 Toroid.seek :clear @12:42:39.33 Carousel.seek 10 @12:42:39.77 Toroid.in :hold @12:42:39.87 Toroid.seek :cartridge @12:42:41.71 Toroid.in :default @12:42:41.80 Toroid.seek :clear @12:42:43.88 Carousel.seek 11 @12:42:44.31 Toroid.in :hold @12:42:44.40 Toroid.seek :cartridge @12:42:46.30 Toroid.in :default @12:42:46.39 Toroid.seek :clear @12:42:48.53 Carousel.seek 12 @12:42:48.96 Toroid.in :hold @12:42:49.06 Toroid.seek :cartridge @12:42:50.91 Toroid.in :default @12:42:51.00 Toroid.seek :clear @12:42:53.10 Carousel.seek 13 @12:42:53.53 Toroid.in :hold @12:42:53.63 Toroid.seek :cartridge @12:42:55.49 Toroid.in :default @12:42:55.58 Toroid.seek :clear @12:42:57.67 Carousel.seek 14 @12:42:58.10 Toroid.in :hold @12:42:58.18 Toroid.seek :cartridge @12:43:00.06 Toroid.in :default @12:43:00.15 Toroid.seek :clear @12:43:02.30 Carousel.seek 15 @12:43:02.73 Toroid.in :hold @12:43:02.82 Toroid.seek :cartridge @12:43:04.68 Toroid.in :default @12:43:04.77 Toroid.seek :clear @12:43:07.40 Carousel.seek 16 @12:43:07.93 Toroid.in :hold @12:43:08.01 Toroid.seek :cartridge @12:43:09.87 Toroid.in :default @12:43:09.96 Toroid.seek :clear @12:43:12.06 Carousel.seek 17 @12:43:12.49 Toroid.in :hold @12:43:12.59 Toroid.seek :cartridge @12:43:14.45 Toroid.in :default @12:43:14.55 Toroid.seek :clear @12:43:16.66 Carousel.seek 18 @12:43:17.09 Toroid.in :hold @12:43:17.18 Toroid.seek :cartridge @12:43:19.05 Toroid.in :default @12:43:19.13 Toroid.seek :clear @12:43:21.24 Carousel.seek 19 @12:43:21.68 Toroid.in :hold @12:43:21.76 Toroid.seek :cartridge @12:43:23.62 Toroid.in :default @12:43:23.70 Toroid.seek :clear @12:43:25.81 Carousel.seek 20 @12:43:26.25 Toroid.in :hold @12:43:26.36 Toroid.seek :cartridge @12:43:28.24 Toroid.in :default @12:43:28.34 Toroid.seek :clear @12:43:30.47 Carousel.seek 21 @12:43:30.90 Toroid.in :hold @12:43:30.99 Toroid.seek :cartridge @12:43:32.86 Toroid.in :default @12:43:32.95 Toroid.seek :clear @12:43:35.03 Carousel.seek 22 @12:43:35.98 Toroid.in :hold @12:43:36.17 Toroid.seek :cartridge @12:43:38.05 Toroid.in :default @12:43:38.14 Toroid.seek :clear @12:43:40.27 Carousel.seek 23 @12:43:40.70 Toroid.in :hold @12:43:40.80 Toroid.seek :cartridge @12:43:42.66 Toroid.in :default @12:43:42.75 Toroid.seek :clear @12:43:44.84 Carousel.seek 24 @12:43:45.27 Toroid.in :hold @12:43:45.36 Toroid.seek :cartridge @12:43:47.23 Toroid.in :default @12:43:47.31 Toroid.seek :clear @12:43:49.42 Carousel.seek 25 @12:43:49.85 Toroid.in :hold @12:43:49.94 Toroid.seek :cartridge @12:43:51.81 Toroid.in :default @12:43:51.88 Toroid.seek :clear @12:43:54.01 Carousel.seek 26 @12:43:54.45 Toroid.in :hold @12:43:54.54 Toroid.seek :cartridge @12:43:56.40 Toroid.in :default @12:43:56.49 Toroid.seek :clear @12:43:58.59 Carousel.seek 27 @12:43:59.02 Toroid.in :hold @12:43:59.11 Toroid.seek :cartridge @12:44:00.99 Toroid.in :default @12:44:01.08 Toroid.seek :clear @12:44:03.17 Carousel.seek 28 @12:44:03.60 Toroid.in :hold @12:44:03.79 Toroid.seek :cartridge @12:44:05.67 Toroid.in :default @12:44:05.76 Toroid.seek :clear @12:44:08.39 Carousel.seek 29 @12:44:08.92 Toroid.in :hold @12:44:09.01 Toroid.seek :cartridge @12:44:10.90 Toroid.in :default @12:44:10.99 Toroid.seek :clear @12:44:13.10 Carousel.seek 30 @12:44:13.54 Toroid.in :hold @12:44:13.63 Toroid.seek :cartridge @12:44:15.50 Toroid.in :default @12:44:15.59 Toroid.seek :clear @12:44:17.70 Carousel.seek 31 @12:44:18.15 Toroid.in :hold @12:44:18.25 Toroid.seek :cartridge @12:44:20.14 Toroid.in :default @12:44:20.25 Toroid.seek :clear @12:44:22.38 Carousel.seek 32 @12:44:22.83 Toroid.in :hold @12:44:22.92 Toroid.seek :cartridge @12:44:24.80 Toroid.in :default @12:44:24.88 Toroid.seek :clear @12:44:26.97 Carousel.seek 33 @12:44:27.42 Toroid.in :hold @12:44:27.51 Toroid.seek :cartridge @12:44:29.40 Toroid.in :default @12:44:29.48 Toroid.seek :clear @12:44:31.58 Carousel.seek 34 @12:44:32.02 Toroid.in :hold @12:44:32.11 Toroid.seek :cartridge @12:44:33.98 Toroid.in :default @12:44:34.07 Toroid.seek :clear @12:44:36.17 Carousel.seek 35 @12:44:36.63 Toroid.in :hold @12:44:36.72 Toroid.seek :cartridge @12:44:39.11 Toroid.in :default @12:44:39.30 Toroid.seek :clear @12:44:41.41 Carousel.seek 36 @12:44:41.85 Toroid.in :hold @12:44:41.94 Toroid.seek :cartridge @12:44:43.81 Toroid.in :default @12:44:43.91 Toroid.seek :clear @12:44:46.01 Carousel.seek 37 @12:44:46.45 Toroid.in :hold @12:44:46.53 Toroid.seek :cartridge @12:44:48.40 Toroid.in :default @12:44:48.49 Toroid.seek :clear @12:44:50.59 Carousel.seek 38 @12:44:51.04 Toroid.in :hold @12:44:51.14 Toroid.seek :cartridge @12:44:53.00 Toroid.in :default @12:44:53.08 Toroid.seek :clear @12:44:55.20 Carousel.seek 39 @12:44:55.65 Toroid.in :hold @12:44:55.74 Toroid.seek :cartridge @12:44:57.62 Toroid.in :default @12:44:57.71 Toroid.seek :clear @12:44:59.82 Carousel.seek 40 @12:45:00.28 Toroid.in :hold @12:45:00.37 Toroid.seek :cartridge @12:45:02.25 Toroid.in :default @12:45:02.35 Toroid.seek :clear @12:45:04.47 Carousel.seek 41 @12:45:04.89 Toroid.in :hold @12:45:04.98 Toroid.seek :cartridge @12:45:06.84 Toroid.in :default @12:45:06.93 Toroid.seek :clear @12:45:09.55 Carousel.seek 42 @12:45:10.08 Toroid.in :hold @12:45:10.16 Toroid.seek :cartridge @12:45:12.04 Toroid.in :default @12:45:12.12 Toroid.seek :clear @12:45:14.27 Carousel.seek 43 @12:45:14.71 Toroid.in :hold @12:45:14.80 Toroid.seek :cartridge @12:45:16.66 Toroid.in :default @12:45:16.75 Toroid.seek :clear @12:45:18.86 Carousel.seek 44 @12:45:19.30 Toroid.in :hold @12:45:19.39 Toroid.seek :cartridge @12:45:21.26 Toroid.in :default @12:45:21.35 Toroid.seek :clear @12:45:23.45 Carousel.seek 45 @12:45:23.88 Toroid.in :hold @12:45:23.98 Toroid.seek :cartridge @12:45:25.85 Toroid.in :default @12:45:25.95 Toroid.seek :clear @12:45:28.04 Carousel.seek 46 @12:45:28.47 Toroid.in :hold @12:45:28.56 Toroid.seek :cartridge @12:45:30.43 Toroid.in :default @12:45:30.53 Toroid.seek :clear @12:45:32.65 Carousel.seek 47 @12:45:33.09 Toroid.in :hold @12:45:33.19 Toroid.seek :cartridge @12:45:35.06 Toroid.in :default @12:45:35.15 Toroid.seek :clear @12:45:37.25 Carousel.seek 48 @12:45:37.67 Toroid.in :hold @12:45:37.75 Toroid.seek :cartridge @12:45:39.62 Toroid.in :default @12:45:40.25 Toroid.seek :clear @12:45:42.49 Carousel.seek 49 @12:45:42.93 Toroid.in :hold @12:45:43.01 Toroid.seek :cartridge @12:45:44.88 Toroid.in :default @12:45:44.97 Toroid.seek :clear @12:45:47.06 Carousel.seek 50 @12:45:47.50 Toroid.in :hold @12:45:47.58 Toroid.seek :cartridge @12:45:49.45 Toroid.in :default @12:45:49.54 Toroid.seek :clear @12:45:51.66 Carousel.seek 51 @12:45:52.09 Toroid.in :hold @12:45:52.18 Toroid.seek :cartridge @12:45:54.09 Toroid.in :default @12:45:54.18 Toroid.seek :clear @12:45:56.30 Carousel.seek 52 @12:45:56.73 Toroid.in :hold @12:45:56.82 Toroid.seek :cartridge @12:45:58.70 Toroid.in :default @12:45:58.79 Toroid.seek :clear @12:46:00.88 Carousel.seek 53 @12:46:01.31 Toroid.in :hold @12:46:01.40 Toroid.seek :cartridge @12:46:03.27 Toroid.in :default @12:46:03.36 Toroid.seek :clear @12:46:05.46 Carousel.seek 54 @12:46:05.94 Toroid.in :hold @12:46:06.03 Toroid.seek :cartridge @12:46:07.90 Toroid.in :default @12:46:07.99 Toroid.seek :clear @12:46:10.09 Carousel.seek 55 @12:46:10.78 Toroid.in :hold @12:46:10.97 Toroid.seek :cartridge @12:46:12.85 Toroid.in :default @12:46:12.93 Toroid.seek :clear @12:46:15.04 Carousel.seek 56 @12:46:15.48 Toroid.in :hold @12:46:15.57 Toroid.seek :cartridge @12:46:17.44 Toroid.in :default @12:46:17.52 Toroid.seek :clear @12:46:19.61 Carousel.seek 57 @12:46:20.04 Toroid.in :hold @12:46:20.13 Toroid.seek :cartridge @12:46:22.00 Toroid.in :default @12:46:22.09 Toroid.seek :clear @12:46:24.18 Carousel.seek 58 @12:46:24.65 Toroid.in :hold @12:46:24.75 Toroid.seek :cartridge @12:46:26.62 Toroid.in :default @12:46:26.71 Toroid.seek :clear @12:46:28.82 Carousel.seek 59 @12:46:29.25 Toroid.in :hold @12:46:29.34 Toroid.seek :cartridge @12:46:31.20 Toroid.in :default @12:46:31.29 Toroid.seek :clear @12:46:33.39 Carousel.seek 60 @12:46:33.82 Toroid.in :hold @12:46:33.91 Toroid.seek :cartridge @12:46:35.78 Toroid.in :default @12:46:35.87 Toroid.seek :clear @12:46:37.70 Sampling 100.0ml @12:46:37.72 SamplePump.setPosition! 0ml @12:46:37.99 Abs.in :default @12:46:38.08 Abs.hold @12:46:38.45 SamplePump.in :start @12:46:38.55 SamplePump.seek 100ml,8:50 @12:46:39.67 SamplePump.configure :sample @12:53:24.38 Abs.stop @12:53:24.54 Intake.close @12:53:24.64 Exhaust.close @12:53:24.88 Toroid.in :hold @12:53:24.97 Toroid.seek :bypass @12:53:26.63 Toroid.in :default @12:53:26.72 Toroid.seek :clear @12:53:28.37 Sampled 100.0ml @12:53:28.44 Ensuring (60) cartridge(s) open @12:53:28.75 Carousel.seek 60 @12:53:28.89 Toroid.in :hold @12:53:28.98 Toroid.seek :cartridge @12:53:30.85 Toroid.in :default @12:53:30.94 Toroid.seek :clear @12:53:32.78 Sample loop filled with Bleach. Move intake to clean water source @12:53:32.79 delay 15:00 @13:08:32.93 Toroid.in :hold @13:08:33.01 Toroid.seek :bypass @13:08:34.67 Toroid.in :default @13:08:34.76 Toroid.seek :clear @13:08:36.41 Priming bypass w/50ml @13:08:36.43 SamplePump.setPosition! 0ml @13:08:36.54 Bypass.open @13:08:37.97 Intake.open @13:08:38.73 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:08:41.35 Vacuum tare is 52% of full scale at 21C (drifted 1%) @13:08:41.52 Abs.in :default @13:08:41.59 Abs.hold @13:08:41.86 SamplePump.in :start @13:08:41.96 SamplePump.seek 50ml,3:50 @13:08:42.55 SamplePump.configure :prime @13:09:43.31 Abs.stop @13:09:43.42 Ensuring (60) cartridge(s) open @13:09:43.74 Carousel.seek 60 @13:09:43.88 Toroid.in :hold @13:09:43.97 Toroid.seek :cartridge @13:09:45.83 Toroid.in :default @13:09:45.92 Toroid.seek :clear @13:09:47.77 Sampling 500.0ml @13:09:47.78 SamplePump.setPosition! 0ml @13:09:47.89 Bypass.close @13:09:47.97 Exhaust.open @13:09:49.46 Abs.in :default @13:09:49.56 Abs.hold @13:09:49.88 SamplePump.in :start @13:09:49.97 SamplePump.seek 500ml,42:10 @13:09:50.58 SamplePump.configure :sample @13:42:37.79 Abs.stop @13:42:37.94 Intake.close @13:42:38.01 Exhaust.close @13:42:38.32 Toroid.in :hold @13:42:38.41 Toroid.seek :bypass @13:42:40.07 Toroid.in :default @13:42:40.16 Toroid.seek :clear @13:42:41.83 Sampled 500.0ml @13:42:41.89 Ensuring (60) cartridge(s) open @13:42:42.25 Carousel.seek 60 @13:42:42.40 Toroid.in :hold @13:42:42.47 Toroid.seek :cartridge @13:42:44.33 Toroid.in :default @13:42:44.42 Toroid.seek :clear @13:42:46.29 Waiting for preston to answer "Rinse complete. Disconnect intake water source and attach air filter." @13:45:21.32 Answered Rinse complete. Disconnect intake water source and attach air filter."" @13:45:21.35 SamplePump.setPosition! 0ml @13:45:21.43 Intake.open @13:45:22.10 Exhaust.open @13:45:22.77 SamplePump.in :slow @13:45:22.85 SamplePump.seek 200ml @13:45:26.02 SamplePump.hold @13:45:26.25 UserAbort in preston -- Sample Pump (SP) seek to 200ml Aborted! @13:51:21.01 -> Thread.resume @13:51:21.07 Resuming after Sample Pump (SP) seek to 200ml Aborted! at 13:45:26 @13:51:21.09 SamplePump.seek 200ml @13:59:26.10 Intake.close @13:59:26.79 Bypass.open @13:59:27.51 Waiting for preston to answer "Hook up vacuum Flask to to Exhaust line, turn on vacuum, remove Fish in slot 60, push return" @14:01:04.63 Session Begun @14:01:09.37 -> SC.to 60 @14:01:09.44 Carousel.seek 60 Storage Carousel (SC) at 60 @14:01:14.34 -> SC Storage Carousel (SC) at 60 @14:02:12.23 Answered Hook up vacuum Flask to to Exhaust line, turn on vacuum, remove Fish in slot 60, push return"" @14:02:12.29 delay 30 seconds @14:02:42.36 Waiting for preston to answer "replace Fish in slot 60, remove fish in slot 1" @14:03:37.91 Answered replace Fish in slot 60, remove fish in slot 1"" @14:03:37.94 delay 30 seconds @14:04:08.03 Waiting for preston to answer "replace Fish in slot 1 and release vacuum" @14:05:05.11 Answered replace Fish in slot 1 and release vacuum"" @14:05:05.15 Ensuring all (60) cartridges are bypassed @14:05:05.60 Carousel.seek 1 @14:05:17.86 Toroid.in :hold @14:05:18.00 Toroid.seek :bypass @14:05:19.71 Toroid.in :default @14:05:19.82 Toroid.seek :clear @14:05:22.38 Carousel.seek 2 @14:05:22.85 Toroid.in :hold @14:05:22.96 Toroid.seek :bypass @14:05:24.64 Toroid.in :default @14:05:24.76 Toroid.seek :clear @14:05:26.69 Carousel.seek 3 @14:05:27.15 Toroid.in :hold @14:05:27.26 Toroid.seek :bypass @14:05:28.94 Toroid.in :default @14:05:29.04 Toroid.seek :clear @14:05:30.98 Carousel.seek 4 @14:05:31.43 Toroid.in :hold @14:05:31.56 Toroid.seek :bypass @14:05:33.24 Toroid.in :default @14:05:33.35 Toroid.seek :clear @14:05:35.29 Carousel.seek 5 @14:05:35.74 Toroid.in :hold @14:05:35.85 Toroid.seek :bypass @14:05:37.53 Toroid.in :default @14:05:37.65 Toroid.seek :clear @14:05:39.58 Carousel.seek 6 @14:05:40.03 Toroid.in :hold @14:05:40.13 Toroid.seek :bypass @14:05:41.83 Toroid.in :default @14:05:41.94 Toroid.seek :clear @14:05:43.84 Carousel.seek 7 @14:05:44.32 Toroid.in :hold @14:05:44.42 Toroid.seek :bypass @14:05:46.12 Toroid.in :default @14:05:46.24 Toroid.seek :clear @14:05:48.17 Carousel.seek 8 @14:05:48.66 Toroid.in :hold @14:05:49.30 Toroid.seek :bypass @14:05:51.08 Toroid.in :default @14:05:51.19 Toroid.seek :clear @14:05:53.13 Carousel.seek 9 @14:05:53.58 Toroid.in :hold @14:05:53.69 Toroid.seek :bypass @14:05:55.37 Toroid.in :default @14:05:55.48 Toroid.seek :clear @14:05:57.42 Carousel.seek 10 @14:05:57.87 Toroid.in :hold @14:05:57.99 Toroid.seek :bypass @14:05:59.68 Toroid.in :default @14:05:59.79 Toroid.seek :clear @14:06:01.71 Carousel.seek 11 @14:06:02.16 Toroid.in :hold @14:06:02.30 Toroid.seek :bypass @14:06:03.99 Toroid.in :default @14:06:04.10 Toroid.seek :clear @14:06:06.02 Carousel.seek 12 @14:06:06.47 Toroid.in :hold @14:06:06.59 Toroid.seek :bypass @14:06:08.31 Toroid.in :default @14:06:08.42 Toroid.seek :clear @14:06:10.38 Carousel.seek 13 @14:06:10.83 Toroid.in :hold @14:06:10.93 Toroid.seek :bypass @14:06:12.62 Toroid.in :default @14:06:12.73 Toroid.seek :clear @14:06:14.66 Carousel.seek 14 @14:06:15.10 Toroid.in :hold @14:06:15.21 Toroid.seek :bypass @14:06:16.89 Toroid.in :default @14:06:17.00 Toroid.seek :clear @14:06:19.45 Carousel.seek 15 @14:06:20.00 Toroid.in :hold @14:06:20.11 Toroid.seek :bypass @14:06:21.79 Toroid.in :default @14:06:21.91 Toroid.seek :clear @14:06:23.83 Carousel.seek 16 @14:06:24.31 Toroid.in :hold @14:06:24.42 Toroid.seek :bypass @14:06:26.10 Toroid.in :default @14:06:26.20 Toroid.seek :clear @14:06:28.16 Carousel.seek 17 @14:06:28.65 Toroid.in :hold @14:06:28.76 Toroid.seek :bypass @14:06:30.42 Toroid.in :default @14:06:30.52 Toroid.seek :clear @14:06:32.49 Carousel.seek 18 @14:06:32.94 Toroid.in :hold @14:06:33.07 Toroid.seek :bypass @14:06:34.76 Toroid.in :default @14:06:34.87 Toroid.seek :clear @14:06:36.78 Carousel.seek 19 @14:06:37.22 Toroid.in :hold @14:06:37.34 Toroid.seek :bypass @14:06:39.03 Toroid.in :default @14:06:39.14 Toroid.seek :clear @14:06:41.05 Carousel.seek 20 @14:06:41.50 Toroid.in :hold @14:06:41.61 Toroid.seek :bypass @14:06:43.30 Toroid.in :default @14:06:43.41 Toroid.seek :clear @14:06:45.33 Carousel.seek 21 @14:06:45.78 Toroid.in :hold @14:06:46.46 Toroid.seek :bypass @14:06:48.27 Toroid.in :default @14:06:48.38 Toroid.seek :clear @14:06:50.34 Carousel.seek 22 @14:06:50.79 Toroid.in :hold @14:06:50.90 Toroid.seek :bypass @14:06:52.59 Toroid.in :default @14:06:52.69 Toroid.seek :clear @14:06:54.61 Carousel.seek 23 @14:06:55.08 Toroid.in :hold @14:06:55.20 Toroid.seek :bypass @14:06:56.91 Toroid.in :default @14:06:57.02 Toroid.seek :clear @14:06:58.96 Carousel.seek 24 @14:06:59.42 Toroid.in :hold @14:06:59.53 Toroid.seek :bypass @14:07:01.23 Toroid.in :default @14:07:01.34 Toroid.seek :clear @14:07:03.26 Carousel.seek 25 @14:07:03.71 Toroid.in :hold @14:07:03.82 Toroid.seek :bypass @14:07:05.51 Toroid.in :default @14:07:05.63 Toroid.seek :clear @14:07:07.56 Carousel.seek 26 @14:07:08.02 Toroid.in :hold @14:07:08.13 Toroid.seek :bypass @14:07:09.86 Toroid.in :default @14:07:09.97 Toroid.seek :clear @14:07:11.89 Carousel.seek 27 @14:07:12.35 Toroid.in :hold @14:07:12.45 Toroid.seek :bypass @14:07:14.14 Toroid.in :default @14:07:14.28 Toroid.seek :clear @14:07:16.76 Carousel.seek 28 @14:07:17.31 Toroid.in :hold @14:07:17.42 Toroid.seek :bypass @14:07:19.10 Toroid.in :default @14:07:19.21 Toroid.seek :clear @14:07:21.13 Carousel.seek 29 @14:07:21.57 Toroid.in :hold @14:07:21.68 Toroid.seek :bypass @14:07:23.36 Toroid.in :default @14:07:23.47 Toroid.seek :clear @14:07:25.39 Carousel.seek 30 @14:07:25.86 Toroid.in :hold @14:07:25.96 Toroid.seek :bypass @14:07:27.64 Toroid.in :default @14:07:27.75 Toroid.seek :clear @14:07:29.67 Carousel.seek 31 @14:07:30.09 Toroid.in :hold @14:07:30.20 Toroid.seek :bypass @14:07:31.91 Toroid.in :default @14:07:32.02 Toroid.seek :clear @14:07:33.93 Carousel.seek 32 @14:07:34.38 Toroid.in :hold @14:07:34.49 Toroid.seek :bypass @14:07:36.17 Toroid.in :default @14:07:36.32 Toroid.seek :clear @14:07:38.30 Carousel.seek 33 @14:07:38.75 Toroid.in :hold @14:07:38.86 Toroid.seek :bypass @14:07:40.54 Toroid.in :default @14:07:40.66 Toroid.seek :clear @14:07:42.58 Carousel.seek 34 @14:07:43.03 Toroid.in :hold @14:07:43.73 Toroid.seek :bypass @14:07:45.47 Toroid.in :default @14:07:45.58 Toroid.seek :clear @14:07:47.51 Carousel.seek 35 @14:07:47.98 Toroid.in :hold @14:07:48.09 Toroid.seek :bypass @14:07:49.78 Toroid.in :default @14:07:49.89 Toroid.seek :clear @14:07:51.80 Carousel.seek 36 @14:07:52.29 Toroid.in :hold @14:07:52.40 Toroid.seek :bypass @14:07:54.10 Toroid.in :default @14:07:54.23 Toroid.seek :clear @14:07:56.18 Carousel.seek 37 @14:07:56.67 Toroid.in :hold @14:07:56.78 Toroid.seek :bypass @14:07:58.48 Toroid.in :default @14:07:58.59 Toroid.seek :clear @14:08:00.54 Carousel.seek 38 @14:08:01.00 Toroid.in :hold @14:08:01.10 Toroid.seek :bypass @14:08:02.78 Toroid.in :default @14:08:02.87 Toroid.seek :clear @14:08:04.80 Carousel.seek 39 @14:08:05.26 Toroid.in :hold @14:08:05.37 Toroid.seek :bypass @14:08:07.06 Toroid.in :default @14:08:07.17 Toroid.seek :clear @14:08:09.08 Carousel.seek 40 @14:08:09.53 Toroid.in :hold @14:08:09.64 Toroid.seek :bypass @14:08:11.32 Toroid.in :default @14:08:11.43 Toroid.seek :clear @14:08:13.88 Carousel.seek 41 @14:08:14.42 Toroid.in :hold @14:08:14.54 Toroid.seek :bypass @14:08:16.24 Toroid.in :default @14:08:16.36 Toroid.seek :clear @14:08:18.29 Carousel.seek 42 @14:08:18.74 Toroid.in :hold @14:08:18.85 Toroid.seek :bypass @14:08:20.54 Toroid.in :default @14:08:20.66 Toroid.seek :clear @14:08:22.58 Carousel.seek 43 @14:08:23.05 Toroid.in :hold @14:08:23.16 Toroid.seek :bypass @14:08:24.84 Toroid.in :default @14:08:24.95 Toroid.seek :clear @14:08:26.87 Carousel.seek 44 @14:08:27.32 Toroid.in :hold @14:08:27.44 Toroid.seek :bypass @14:08:29.12 Toroid.in :default @14:08:29.24 Toroid.seek :clear @14:08:31.16 Carousel.seek 45 @14:08:31.61 Toroid.in :hold @14:08:31.71 Toroid.seek :bypass @14:08:33.40 Toroid.in :default @14:08:33.51 Toroid.seek :clear @14:08:35.43 Carousel.seek 46 @14:08:35.87 Toroid.in :hold @14:08:35.97 Toroid.seek :bypass @14:08:37.67 Toroid.in :default @14:08:37.78 Toroid.seek :clear @14:08:39.70 Carousel.seek 47 @14:08:40.73 Toroid.in :hold @14:08:40.94 Toroid.seek :bypass @14:08:42.63 Toroid.in :default @14:08:42.74 Toroid.seek :clear @14:08:44.68 Carousel.seek 48 @14:08:45.13 Toroid.in :hold @14:08:45.24 Toroid.seek :bypass @14:08:46.93 Toroid.in :default @14:08:47.04 Toroid.seek :clear @14:08:48.96 Carousel.seek 49 @14:08:49.42 Toroid.in :hold @14:08:49.53 Toroid.seek :bypass @14:08:51.21 Toroid.in :default @14:08:51.32 Toroid.seek :clear @14:08:53.24 Carousel.seek 50 @14:08:53.70 Toroid.in :hold @14:08:53.80 Toroid.seek :bypass @14:08:55.51 Toroid.in :default @14:08:55.62 Toroid.seek :clear @14:08:57.51 Carousel.seek 51 @14:08:57.96 Toroid.in :hold @14:08:58.07 Toroid.seek :bypass @14:08:59.77 Toroid.in :default @14:08:59.88 Toroid.seek :clear @14:09:01.80 Carousel.seek 52 @14:09:02.29 Toroid.in :hold @14:09:02.41 Toroid.seek :bypass @14:09:04.10 Toroid.in :default @14:09:04.21 Toroid.seek :clear @14:09:06.17 Carousel.seek 53 @14:09:06.65 Toroid.in :hold @14:09:06.77 Toroid.seek :bypass @14:09:08.45 Toroid.in :default @14:09:08.56 Toroid.seek :clear @14:09:11.05 Carousel.seek 54 @14:09:11.59 Toroid.in :hold @14:09:11.70 Toroid.seek :bypass @14:09:13.39 Toroid.in :default @14:09:13.51 Toroid.seek :clear @14:09:15.43 Carousel.seek 55 @14:09:15.86 Toroid.in :hold @14:09:15.97 Toroid.seek :bypass @14:09:17.65 Toroid.in :default @14:09:17.76 Toroid.seek :clear @14:09:19.69 Carousel.seek 56 @14:09:20.14 Toroid.in :hold @14:09:20.28 Toroid.seek :bypass @14:09:21.97 Toroid.in :default @14:09:22.08 Toroid.seek :clear @14:09:24.00 Carousel.seek 57 @14:09:24.45 Toroid.in :hold @14:09:24.57 Toroid.seek :bypass @14:09:26.29 Toroid.in :default @14:09:26.40 Toroid.seek :clear @14:09:28.35 Carousel.seek 58 @14:09:28.80 Toroid.in :hold @14:09:28.91 Toroid.seek :bypass @14:09:30.59 Toroid.in :default @14:09:30.70 Toroid.seek :clear @14:09:32.62 Carousel.seek 59 @14:09:33.08 Toroid.in :hold @14:09:33.19 Toroid.seek :bypass @14:09:34.87 Toroid.in :default @14:09:34.98 Toroid.seek :clear @14:09:36.90 Carousel.seek 60 @14:09:37.90 Toroid.in :hold @14:09:38.12 Toroid.seek :bypass @14:09:39.80 Toroid.in :default @14:09:39.91 Toroid.seek :clear @14:09:41.56 All dry cartridges bypassed. Sample loop and cartridge ports cleaned and dry. @14:09:41.57 Ready to load cartridges. After cartridges loaded run vacLeakTest @14:11:55.20 -> SC.coast @14:11:55.22 Carousel.coast Storage Carousel (SC) at 60 @08:25:51.67PDT23-Jul-24 -> ESP.reready! @08:25:51.86 Plunge.in :default @08:25:51.99 Toroid.in :default @08:25:52.06 Carousel.in :default @08:25:52.14 Plunge.home.jog 0 @08:25:52.39 Archive.in :default @08:25:52.57 Lysis1.in :default @08:25:52.63 Air.in :default @08:25:52.69 Lysis2.in :default @08:25:52.79 Toroid.home.jog 0 @08:25:52.88 PinPort.in :default @08:25:52.95 Carousel.home.jog 0 @08:25:53.16 Archive.home.jog 0 @08:25:53.51 Lysis1.home.jog 0 @08:25:53.68 Air.home.jog 0 @08:25:53.74 PinPort.home.jog 0 @08:25:53.86 Lysis2.home.jog 0 @08:25:54.73 Plunge.in :homing @08:25:55.00 Plunge.jog 156 @08:25:55.10 PinPort.in :homing @08:25:55.17 Lysis1.in :homing @08:25:55.25 Archive.in :homing @08:25:55.33 Air.in :homing @08:25:55.39 Toroid.in :homing @08:25:55.46 Lysis2.in :homing @08:25:55.80 PinPort.jog -110 @08:25:55.89 Lysis1.jog 1390 @08:25:56.11 Lysis2.jog 1400 @08:25:56.16 Air.jog 1363 @08:25:56.22 Archive.jog 1394 @08:25:56.35 Toroid.jog 624 @08:25:56.84 PinPort.setRawPosition! 0 @08:25:56.87 Plunge.setRawPosition! 50 @08:25:57.07 Plunge.seek :home @08:25:57.10 PinPort.seek :home @08:25:57.36 PinPort.in :default @08:25:57.49 Plunge.in :default @08:25:57.64 Lysis1.setRawPosition! 50 @08:25:57.75 Lysis1.seek :home @08:25:57.89 Lysis2.setRawPosition! 50 @08:25:57.95 Air.setRawPosition! 50 @08:25:58.02 Archive.setRawPosition! 50 @08:25:58.26 Air.seek :home @08:25:58.34 Lysis2.seek :home @08:25:58.52 Archive.seek :home @08:25:58.81 Lysis2.in :default @08:25:58.86 Lysis1.in :default @08:25:58.91 Air.in :default @08:25:58.99 Toroid.setRawPosition! 0 @08:25:59.26 Toroid.seek :home @08:25:59.43 Toroid.in :default @08:25:59.45 Archive.in :default @08:25:59.92 Toroid.seek :clear @08:26:01.69 Carousel.in :default @08:26:02.45 Carousel.home.seek 6 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 200ml Intake is closed Exhaust is opened Bypass = Bypass (PB) is opened Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 6 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @08:26:05.23 -> MainThread.exit @08:26:05.43 Exhaust.close @08:26:05.47 Bypass.close # @08:26:06.37 Session Ended @08:26:06.44 Session Ended @06:09:14.68PDT30-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @06:09:16.54 >BREAK< ESP3 Gateway v1.43 for core bus @06:09:16.57 >BREAK< ESP3 Gateway v1.43 for cartridge bus @06:09:21.08 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @06:09:21.76 Sea pressure (Abs) tare is 6% of full scale @06:09:28.29 Polling Can every 2:00 @06:09:28.34 Waiting for espclients... @06:09:28.75 Session Begun @06:09:42.49 -> ESP.ready! @06:09:42.71 Gate.power :sampler,:OFF,limit to 3500mA @06:09:42.73 Gate.power :skimmer,:OFF,limit to 3500mA @06:09:42.76 Gate.power :analytic1,:ON,limit to 4000mA @06:09:43.53 Plunge.configure :default @06:09:43.71 SamplePump.configure :default @06:09:43.89 Toroid.configure :default @06:09:43.99 Toroid.hold @06:09:44.59 Toroid.configure :default @06:09:45.05 Carousel.configure :default @06:09:45.15 Carousel.hold @06:09:45.25 Carousel.configure :default @06:09:45.42 Abs.configure :default @06:09:45.61 PhaseSeparator.configure :default @06:09:45.79 Archive.configure :default @06:09:45.97 Air.configure :default @06:09:46.13 Lysis1.configure :default @06:09:46.28 Lysis2.configure :default @06:09:46.50 PinPort.configure :default @06:09:46.84 All elf controllers running firmware version 4.92 @06:09:48.32 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @06:09:48.35 AnalyticModule::Unknown in ben -- SPR @06:09:49.16 Toroid.seek :clear @06:09:49.39 Carousel.in :default @06:09:49.56 Carousel.home.seek 45 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 150ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 45 Abs = Absolute Pressure (Leak) Sensor @14psia PhaseSeparator = Intake Head Pressure @0.13psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @06:10:24.65 -> require 'vacuumCart' true @06:10:46.82 -> Power.skimmer :on @06:10:46.85 Gate.power :skimmer,:ON true @06:12:03.01 -> 3.times{refillSampleLines} @06:12:03.04 Refilling sample & bypass lines with fluid. @06:12:03.90 Toroid.seek :clear @06:12:04.12 Carousel.in :default @06:12:04.26 Carousel.home.seek 45 @06:12:04.57 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:12:06.51 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:12:06.52 Bypass.open @06:12:07.27 Intake.open @06:12:07.95 SamplePump.setPosition! 0ml @06:12:08.12 SamplePump.seek 30ml @06:12:45.32 Bypass.close @06:12:46.02 Exhaust.open @06:12:46.74 SamplePump.seek 55ml @06:13:17.85 delay 1 second @06:13:18.88 Intake.close @06:13:18.93 Exhaust.close @06:13:19.71 Sampling Loop filled with water @06:13:19.73 Refilling sample & bypass lines with fluid. @06:13:21.16 Toroid.seek :clear @06:13:21.35 Carousel.in :default @06:13:21.51 Carousel.home.seek 45 @06:13:21.81 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:13:21.87 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:13:21.88 Bypass.open @06:13:22.63 Intake.open @06:13:23.35 SamplePump.setPosition! 0ml @06:13:23.49 SamplePump.seek 30ml @06:14:00.74 Bypass.close @06:14:01.44 Exhaust.open @06:14:02.25 SamplePump.seek 55ml @06:14:33.37 delay 1 second @06:14:34.43 Intake.close @06:14:34.53 Exhaust.close @06:14:35.36 Sampling Loop filled with water @06:14:35.38 Refilling sample & bypass lines with fluid. @06:14:36.23 Toroid.seek :clear @06:14:36.48 Carousel.in :default @06:14:36.62 Carousel.home.seek 45 @06:14:36.91 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:14:36.97 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:14:36.99 Bypass.open @06:14:37.67 Intake.open @06:14:38.43 SamplePump.setPosition! 0ml @06:14:38.60 SamplePump.seek 30ml @06:15:15.80 Bypass.close @06:15:16.50 Exhaust.open @06:15:17.25 SamplePump.seek 55ml @06:15:48.37 delay 1 second @06:15:49.40 Intake.close @06:15:49.51 Exhaust.close @06:15:50.24 Sampling Loop filled with water 3 @06:16:06.83 -> BACsampler.source = skimmer :skimmer @06:16:39.43 -> BACsampler.prime @06:16:39.56 Priming sample loop w/150ml, bypass w/50ml @06:16:39.59 SamplePump.setPosition! 0ml @06:16:39.70 Bypass.open @06:16:41.15 Intake.open @06:16:41.94 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:16:44.58 Vacuum tare is 52% of full scale at 24C (drifted 0%) @06:16:44.77 Abs.in :default @06:16:44.85 Abs.hold @06:16:44.97 PhaseSeparator.in :full @06:16:45.05 PhaseSeparator.hold @06:16:45.34 SamplePump.in :start @06:16:45.43 SamplePump.seek 50ml,3:50 @06:16:46.03 SamplePump.configure :prime @06:17:46.80 Abs.stop @06:17:47.34 PhaseSeparator.stop @06:17:47.53 SamplePump.setPosition! 0ml @06:17:47.63 Exhaust.open @06:17:49.02 Bypass.close @06:17:50.58 Abs.in :default @06:17:50.66 Abs.hold @06:17:50.77 PhaseSeparator.hold @06:17:51.13 SamplePump.in :start @06:17:51.22 SamplePump.seek 150ml,10:30 @06:17:51.82 SamplePump.configure :prime @06:20:54.57 Abs.stop @06:20:54.65 PhaseSeparator.stop BACsampler @06:21:07.50 -> Power.skimmer :off @06:21:07.52 Gate.power :skimmer,:OFF false @06:21:54.63 -> Power.sampler :on @06:21:54.68 Gate.power :sampler,:ON true @06:22:21.96 -> refillSampleLines @06:22:21.98 Refilling sample & bypass lines with fluid. @06:22:22.88 Toroid.seek :clear @06:22:23.09 Carousel.in :default @06:22:23.23 Carousel.home.seek 45 @06:22:23.53 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:22:27.05 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:22:27.09 Bypass.open @06:22:27.82 SamplePump.setPosition! 0ml @06:22:27.97 SamplePump.in :default @06:22:28.05 SamplePump.seek 30ml @06:23:05.26 Bypass.close @06:23:06.02 SamplePump.seek 55ml @06:23:37.12 delay 1 second @06:23:38.15 Intake.close @06:23:38.24 Exhaust.close @06:23:39.11 Sampling Loop filled with water @06:24:07.63 -> BACsampler.source=sampler :sampler @06:24:19.79 -> BACsampler.prime @06:24:19.93 Priming sample loop w/150ml, bypass w/50ml @06:24:19.95 SamplePump.setPosition! 0ml @06:24:20.05 Bypass.open @06:24:21.59 Intake.open @06:24:23.14 Abs.in :default @06:24:23.21 Abs.hold @06:24:23.33 PhaseSeparator.hold @06:24:23.63 SamplePump.in :start @06:24:23.73 SamplePump.seek 50ml,3:50 @06:24:24.33 SamplePump.configure :prime @06:25:25.12 Abs.stop @06:25:25.21 PhaseSeparator.stop @06:25:25.29 SamplePump.setPosition! 0ml @06:25:25.37 Exhaust.open @06:25:26.75 Bypass.close @06:25:28.34 Abs.in :default @06:25:28.47 Abs.hold @06:25:28.59 PhaseSeparator.hold @06:25:29.53 SamplePump.in :start @06:25:29.63 SamplePump.seek 150ml,10:30 @06:25:30.21 SamplePump.configure :prime @06:28:33.03 Abs.stop @06:28:33.11 PhaseSeparator.stop BACsampler @06:29:34.56 -> Tweaks.clear {} @06:31:28.35 -> fire({source:deep}, archiveNull_bac, {source:scum}, archiveNull_bac) # @06:31:29.46 Toroid.seek :clear @06:31:29.70 Carousel.in :default @06:31:29.86 Carousel.home.seek 45 @06:31:30.15 Selecting Cartridge 35 @06:31:30.51 Carousel.seek 35 @06:31:32.91 Lysis1.in :fast @06:31:33.01 Lysis1.seek :locked @06:31:35.68 Plunge.seek :locked @06:31:35.88 Air.in :fast @06:31:35.99 Air.seek :locked @06:31:36.99 Carousel.reconfigure :locked @06:31:37.17 Powering cartridge bus @06:31:39.86 Gate.power :cartridge,:OFF @06:31:40.45 Mounted archive Cartridge @06:31:40.55 BACsampler with {source: :sampler} @06:31:40.62 GoodNews.email "BACsampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling archiveNull_bac cartridge in slot 35" @06:31:41.17 Preparing to filter cartridge 35 @06:31:41.52 Gate.power :sampler,:OFF @06:31:41.56 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:31:47.05 upsync @06:32:23.21 Intake Head Pressure tare is 3% of full scale at 24C (drifted 2%) @06:32:23.23 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:32:23.32 PhaseSeparator.in :filling @06:32:23.39 PhaseSeparator.hold @06:32:23.51 Gate.power :sampler,:ON @06:32:31.87 Head pressure reached 1.19psi in 9 seconds @06:32:31.94 Bypass.open @06:32:32.63 Intake.close @06:32:33.45 Toroid.in :hold @06:32:33.55 Toroid.seek :crack @06:32:35.15 Toroid.seek :cartridge @06:32:36.30 Toroid.in :default @06:32:36.39 Toroid.seek :clear @06:32:38.55 Pressurizing cartridge to 26psi above 13.8psia @06:32:38.57 SamplePump.setPosition! 0ml @06:32:38.67 Intake.open @06:32:39.38 Bypass.close @06:32:40.10 Exhaust.close @06:32:40.91 Abs.in :default @06:32:41.00 Abs.hold @06:32:41.12 PhaseSeparator.in :full @06:32:41.21 PhaseSeparator.hold @06:32:41.49 Waiting for sampler to finish priming... @06:32:41.51 delayUntil "06:33:53.53" @06:33:53.59 SamplePump.in :charge @06:33:53.69 SamplePump.seek 2.3ml,42 seconds @06:34:08.81 Abs.stop @06:34:08.91 PhaseSeparator.stop @06:34:09.02 Intake.close @06:34:09.81 Pressurized cartridge with 1.026ml to 26.1psi @06:34:09.83 Isolated @37.8psia (24.0psi) @06:34:09.85 Verifying cartridge holds at least 0.65..0.85 of 37.8psia @06:34:09.96 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:34:14.56 Cartridge pressure fell 6% to 35.6psia @06:34:14.69 Sampling 1000.0ml @06:34:14.71 SamplePump.setPosition! 0ml @06:34:14.83 Exhaust.open @06:34:15.46 Intake.open @06:34:16.36 Abs.in :default @06:34:17.09 Abs.hold @06:34:17.21 PhaseSeparator.hold @06:34:17.66 SamplePump.in :start @06:34:17.78 SamplePump.seek 1000ml,1:23:50 @06:34:18.35 SamplePump.configure :sample @06:34:20.76 Cartridge 35 filtering 1/1000ml at 0.61ml/s with 13.0psi vacuum 6.5m deep (24.3psia) @06:35:20.83 Cartridge 35 filtering 30/1000ml at 0.47ml/s with 17.0psi vacuum 6.3m deep (24.0psia) @06:36:20.82 Cartridge 35 filtering 57/1000ml at 0.46ml/s with 22.8psi vacuum @06:37:20.81 Cartridge 35 filtering 84/1000ml at 0.45ml/s with 23.5psi vacuum @06:38:20.82 Cartridge 35 filtering 111/1000ml at 0.44ml/s with 12.0psi vacuum 11.6m deep (31.8psia) @06:38:39.15 -> Cmd.stop @06:38:39.18 Abs.stop @06:38:39.26 PhaseSeparator.stop @06:38:39.48 Exhaust.close @06:38:39.64 Intake.close @06:38:39.83 Toroid.in :hold @06:38:39.92 Toroid.seek :bypass @06:38:41.57 Toroid.in :default @06:38:41.65 Toroid.seek :clear @06:38:43.85 Carousel.in :default @06:38:44.09 GoodNews.email "After filtering 118.6ml",Subject: "Processing cartridge in slot 35" @06:38:44.18 Cartridge::Error in detached from ben -- Not Mounted @06:38:44.22 Carousel.home.seek 35 @06:38:44.27 Waiting to Resume from Checkpoint "Selecting Cartridge 35" @06:38:44.71 Plunge.seek :locked @06:38:45.05 Air.in :unlocking @06:38:45.23 Air.seek :clear @06:38:45.39 Lysis1.in :unlocking @06:38:45.56 Lysis1.seek :clear @06:38:46.45 Gate.power :sampler,:OFF @06:38:47.11 Gate.power :analytic1,:OFF @06:38:47.13 Gate.power :motor,:OFF @06:38:47.17 Gate.power :core,:OFF @06:38:47.21 Gate.power :main,:OFF @06:38:47.29 Safely stopped and ready to power off @06:38:47.64 BadNews.email "Not Mounted",Subject: "Cartridge::Error in detached from ben" :STOPPED @06:38:50.38 upsync @06:38:54.12 upsync @06:44:23.45 -> slot 35=>[:dry,:archiveNull_bac] {35 => { Type: :archiveNull_bac, State: :dry}} @06:44:57.02 -> fire({source:deep}, archiveNull_bac, archiveNull_bac, {source:scum}, archiveNull_bac, archiveNull_bac) # @06:44:57.25 Gate.power :sampler,:OFF,limit to 3500mA @06:44:57.31 Gate.power :skimmer,:OFF,limit to 3500mA @06:44:57.36 Gate.power :main,:ON,limit to 5000mA @06:44:57.40 Gate.power :analytic1,:ON,limit to 4000mA @06:44:57.53 Powering core bus @06:45:00.33 Plunge.configure :default @06:45:00.48 SamplePump.configure :default @06:45:00.61 Toroid.configure :default @06:45:00.73 Carousel.configure :default @06:45:00.85 Abs.configure :default @06:45:00.95 PhaseSeparator.configure :full @06:45:01.06 Archive.configure :default @06:45:01.18 Air.configure :default @06:45:01.53 Lysis1.configure :default @06:45:01.62 Lysis2.configure :default @06:45:01.73 PinPort.configure :default @06:45:01.81 All elf controllers running firmware version 4.92 @06:45:01.85 Gate.power :motor,:ON,limit to 5000mA @06:45:04.77 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @06:45:04.80 AnalyticModule::Unknown in detached from ben#2 -- SPR @06:45:05.05 Plunge.home.jog 0 @06:45:05.89 Lysis2.home.jog 0 @06:45:05.95 Air.home.jog 0 @06:45:05.99 Carousel.home.jog 0 @06:45:06.08 Lysis1.home.jog 0 @06:45:06.12 Toroid.home.jog 0 @06:45:06.17 PinPort.home.jog 0 @06:45:06.23 Archive.home.jog 0 @06:45:07.05 Plunge.in :homing @06:45:07.37 Plunge.jog 1604 @06:45:07.65 PinPort.in :homing @06:45:07.74 Lysis2.in :homing @06:45:07.80 Air.in :homing @06:45:07.83 Toroid.in :homing @06:45:07.92 Lysis1.in :homing @06:45:08.02 Archive.in :homing @06:45:08.19 PinPort.jog -110 @06:45:08.34 Lysis2.jog 1404 @06:45:08.48 Lysis1.jog 1396 @06:45:08.56 Air.jog 1365 @06:45:08.61 Archive.jog 1395 @06:45:08.70 Toroid.jog 621 @06:45:09.14 Plunge.setRawPosition! 50 @06:45:09.17 PinPort.setRawPosition! 0 @06:45:09.34 PinPort.seek :home @06:45:09.37 Plunge.seek :home @06:45:09.62 PinPort.in :default @06:45:09.80 Plunge.in :default @06:45:09.93 Lysis2.setRawPosition! 50 @06:45:10.03 Lysis2.seek :home @06:45:10.20 Air.setRawPosition! 50 @06:45:10.24 Archive.setRawPosition! 50 @06:45:10.29 Lysis1.setRawPosition! 50 @06:45:10.52 Air.seek :home @06:45:10.58 Lysis1.seek :home @06:45:10.64 Archive.seek :home @06:45:11.74 Lysis1.in :default @06:45:11.80 Air.in :default @06:45:11.85 Toroid.setRawPosition! 0 @06:45:11.90 Lysis2.in :default @06:45:11.94 Archive.in :default @06:45:12.28 Toroid.seek :home @06:45:12.48 Toroid.in :default @06:45:12.64 Toroid.seek :clear @06:45:14.43 Carousel.in :default @06:45:14.62 Carousel.home.seek 35 @06:45:14.96 Selecting Cartridge 35 @06:45:15.33 Carousel.seek 35 @06:45:15.63 Lysis1.in :fast @06:45:15.72 Lysis1.seek :locked @06:45:18.41 Plunge.seek :locked @06:45:18.64 Air.in :fast @06:45:18.74 Air.seek :locked @06:45:19.75 Carousel.reconfigure :locked @06:45:19.99 Powering cartridge bus @06:45:22.69 Gate.power :cartridge,:OFF @06:45:23.28 Mounted archive Cartridge @06:45:23.37 BACsampler with {source: :sampler} @06:45:23.39 GoodNews.email "BACsampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling archiveNull_bac cartridge in slot 35" @06:45:23.79 Preparing to filter cartridge 35 @06:45:24.15 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:45:28.77 upsync @06:45:30.79 Intake Head Pressure tare is 3% of full scale at 24C (drifted 2%) @06:45:30.81 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:45:30.91 PhaseSeparator.in :filling @06:45:30.99 PhaseSeparator.hold @06:45:31.66 Gate.power :sampler,:ON @06:45:39.86 Head pressure reached 1.11psi in 9 seconds @06:45:39.98 Priming sample loop w/150ml, bypass w/50ml @06:45:40.00 SamplePump.setPosition! 0ml @06:45:40.11 Bypass.open @06:45:41.61 Intake.open @06:45:42.47 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:45:45.10 Vacuum tare is 52% of full scale at 24C (drifted 0%) @06:45:45.29 Abs.in :default @06:45:45.38 Abs.hold @06:45:45.49 PhaseSeparator.in :full @06:45:45.58 PhaseSeparator.hold @06:45:45.87 Waiting for sampler to finish priming... @06:45:45.89 delayUntil "06:47:01.69" @06:47:01.75 SamplePump.in :start @06:47:01.85 SamplePump.seek 50ml,3:50 @06:47:02.45 SamplePump.configure :prime @06:48:03.25 Abs.stop @06:48:03.35 PhaseSeparator.stop @06:48:03.44 SamplePump.setPosition! 0ml @06:48:03.52 Exhaust.open @06:48:04.92 Bypass.close @06:48:06.53 Abs.in :default @06:48:06.62 Abs.hold @06:48:06.74 PhaseSeparator.hold @06:48:07.12 SamplePump.in :start @06:48:07.23 SamplePump.seek 150ml,10:30 @06:48:07.81 SamplePump.configure :prime @06:50:58.07 -> BACsampler.goalVolume=100 100 @06:51:10.65 Abs.stop @06:51:10.75 PhaseSeparator.stop @06:51:10.85 Bypass.open @06:51:11.58 Intake.close @06:51:12.45 Toroid.in :hold @06:51:12.53 Toroid.seek :crack @06:51:14.12 Toroid.seek :cartridge @06:51:15.26 Toroid.in :default @06:51:15.37 Toroid.seek :clear @06:51:17.46 Pressurizing cartridge to 26psi above 13.9psia @06:51:17.48 SamplePump.setPosition! 0ml @06:51:17.59 Intake.open @06:51:18.28 Bypass.close @06:51:18.98 Exhaust.close @06:51:19.77 Abs.in :default @06:51:19.85 Abs.hold @06:51:19.96 PhaseSeparator.hold @06:51:20.81 SamplePump.in :charge @06:51:21.01 SamplePump.seek 2.3ml,42 seconds @06:51:31.98 Abs.stop @06:51:32.07 PhaseSeparator.stop @06:51:32.17 Intake.close @06:51:32.96 Pressurized cartridge with 0.537ml to 26.5psi @06:51:32.98 Isolated @38.2psia (24.3psi) @06:51:33.02 Verifying cartridge holds at least 0.65..0.85 of 38.2psia @06:51:33.09 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @06:51:37.80 Sampling 1000.0ml @06:51:37.82 SamplePump.setPosition! 0ml @06:51:37.93 Exhaust.open @06:51:38.56 Intake.open @06:51:39.48 Abs.in :default @06:51:39.57 Abs.hold @06:51:39.69 PhaseSeparator.hold @06:51:40.07 SamplePump.in :start @06:51:40.19 SamplePump.seek 1000ml,1:23:50 @06:51:40.76 SamplePump.configure :sample @06:51:43.18 Cartridge 35 filtering 1/1000ml at 0.35ml/s with 28.1psi vacuum @06:52:43.25 Cartridge 35 filtering 29/1000ml at 0.45ml/s with 25.6psi vacuum @06:53:43.23 Cartridge 35 filtering 55/1000ml at 0.44ml/s with 21.4psi vacuum @06:54:43.23 Cartridge 35 filtering 62/1000ml at 0.11ml/s with 23.9psi vacuum @06:54:43.31 SamplePump.stop @06:54:43.44 Intake.close @06:54:43.56 Exhaust.close @06:54:43.83 Toroid.in :hold @06:54:43.94 Toroid.seek :bypass @06:54:45.62 Toroid.in :default @06:54:45.75 Toroid.seek :clear @06:54:47.40 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @06:54:47.42 Abs.stop @06:54:47.53 PhaseSeparator.stop @06:54:47.80 Sampled 61.6ml @06:54:48.31 GoodNews.email "After filtering 61.6ml",Subject: "Processing archiveNull_bac cartridge in slot 35" @06:54:49.53 Plunge.seek :reagent @06:54:50.43 Sample loop closed. Ready to Deliver Reagents @06:54:50.63 Selecting Cartridge 25 @06:54:50.93 Carousel.in :default @06:54:51.14 Carousel.home.seek 35 @06:54:51.25 Gate.power :sampler,:OFF @06:54:51.61 Air.in :unlocking @06:54:51.76 Air.seek :clear @06:54:52.85 Lysis1.in :unlocking @06:54:53.07 Lysis1.seek :clear @06:54:53.15 Plunge.in :unlocking @06:54:53.32 Plunge.seek :clear @06:54:54.59 upsync @06:54:54.78 Carousel.seek 25 @06:54:57.23 Lysis1.in :fast @06:54:57.35 Lysis1.seek :locked @06:55:00.02 Plunge.in :default @06:55:00.12 Plunge.seek :locked @06:55:00.35 Air.in :fast @06:55:00.47 Air.seek :locked @06:55:01.48 Carousel.reconfigure :locked @06:55:01.67 Powering cartridge bus @06:55:04.36 Gate.power :cartridge,:OFF @06:55:04.97 Mounted archive Cartridge @06:55:05.06 BACsampler with {source: :sampler} @06:55:05.08 GoodNews.email "BACsampler with {source: :sampler} filtering up to 100 ml",Subject: "Sampling archiveNull_bac cartridge in slot 25" @06:55:05.49 Preparing to filter cartridge 25 @06:55:05.87 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:55:11.09 upsync @06:55:12.54 Intake Head Pressure tare is 2% of full scale at 24C (drifted 2%) @06:55:12.56 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:55:12.63 PhaseSeparator.in :filling @06:55:12.70 PhaseSeparator.hold @06:55:12.82 Gate.power :sampler,:ON @06:55:20.85 Head pressure reached 1.25psi in 9 seconds @06:55:20.97 Priming sample loop w/150ml, bypass w/50ml @06:55:21.00 SamplePump.setPosition! 0ml @06:55:21.11 Bypass.open @06:55:22.55 Intake.open @06:55:23.32 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:55:25.95 Vacuum tare is 52% of full scale at 24C (drifted 0%) @06:55:26.13 Abs.in :default @06:55:26.21 Abs.hold @06:55:26.34 PhaseSeparator.in :full @06:55:26.47 PhaseSeparator.hold @06:55:26.74 Waiting for sampler to finish priming... @06:55:26.76 delayUntil "06:56:42.84" @06:56:42.92 SamplePump.in :start @06:56:43.03 SamplePump.seek 50ml,3:50 @06:56:43.60 SamplePump.configure :prime @06:57:44.46 Abs.stop @06:57:44.55 PhaseSeparator.stop @06:57:44.65 SamplePump.setPosition! 0ml @06:57:44.75 Exhaust.open @06:57:46.14 Bypass.close @06:57:47.70 Abs.in :default @06:57:47.82 Abs.hold @06:57:47.95 PhaseSeparator.hold @06:57:48.33 SamplePump.in :start @06:57:48.45 SamplePump.seek 150ml,10:30 @06:57:49.02 SamplePump.configure :prime @07:00:51.90 Abs.stop @07:00:51.99 PhaseSeparator.stop @07:00:52.10 Bypass.open @07:00:52.81 Intake.close @07:00:53.61 Toroid.in :hold @07:00:53.70 Toroid.seek :crack @07:00:55.29 Toroid.seek :cartridge @07:00:57.00 Toroid.in :default @07:00:57.20 Toroid.seek :clear @07:00:59.27 Pressurizing cartridge to 26psi above 14.0psia @07:00:59.30 SamplePump.setPosition! 0ml @07:00:59.42 Intake.open @07:01:00.10 Bypass.close @07:01:00.82 Exhaust.close @07:01:01.63 Abs.hold @07:01:01.75 PhaseSeparator.hold @07:01:02.05 SamplePump.in :charge @07:01:02.16 SamplePump.seek 2.3ml,42 seconds @07:01:17.27 Abs.stop @07:01:17.37 PhaseSeparator.stop @07:01:17.47 Intake.close @07:01:18.31 Pressurized cartridge with 1.028ml to 26.4psi @07:01:18.33 Isolated @37.9psia (23.9psi) @07:01:18.39 Verifying cartridge holds at least 0.65..0.85 of 37.9psia @07:01:18.50 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:01:23.20 Sampling 100.0ml @07:01:23.22 SamplePump.setPosition! 0ml @07:01:23.33 Exhaust.open @07:01:24.00 Intake.open @07:01:24.93 Abs.in :default @07:01:25.01 Abs.hold @07:01:25.14 PhaseSeparator.hold @07:01:25.52 SamplePump.in :start @07:01:25.62 SamplePump.seek 100ml,8:50 @07:01:26.21 SamplePump.configure :sample @07:01:28.62 Cartridge 25 filtering 1/100ml at 0.41ml/s with 21.6psi vacuum @07:02:28.69 Cartridge 25 filtering 30/100ml at 0.47ml/s with 25.4psi vacuum @07:03:28.70 Cartridge 25 filtering 57/100ml at 0.46ml/s with 24.7psi vacuum @07:04:28.68 Cartridge 25 filtering 84/100ml at 0.45ml/s with 23.0psi vacuum @07:05:04.72 Abs.stop @07:05:04.81 PhaseSeparator.stop @07:05:05.00 Intake.close @07:05:05.69 Exhaust.close @07:05:06.01 Toroid.in :hold @07:05:06.11 Toroid.seek :bypass @07:05:07.79 Toroid.in :default @07:05:07.88 Toroid.seek :clear @07:05:09.54 Sampled 100.0ml @07:05:10.02 GoodNews.email "After filtering 100.0ml",Subject: "Processing archiveNull_bac cartridge in slot 25" @07:05:10.68 Plunge.seek :reagent @07:05:11.58 Sample loop closed. Ready to Deliver Reagents @07:05:11.76 Selecting Cartridge 15 @07:05:12.09 Carousel.in :default @07:05:12.28 Carousel.home.seek 25 @07:05:12.81 Gate.power :sampler,:OFF @07:05:12.91 Air.in :unlocking @07:05:13.08 Air.seek :clear @07:05:14.18 Lysis1.in :unlocking @07:05:14.36 Lysis1.seek :clear @07:05:14.47 Plunge.in :unlocking @07:05:14.67 Plunge.seek :clear @07:05:15.43 upsync @07:05:16.13 Carousel.seek 15 @07:05:18.57 Lysis1.in :fast @07:05:18.66 Lysis1.seek :locked @07:05:21.30 Plunge.in :default @07:05:21.39 Plunge.seek :locked @07:05:21.60 Air.in :fast @07:05:21.70 Air.seek :locked @07:05:22.69 Carousel.reconfigure :locked @07:05:22.90 Powering cartridge bus @07:05:25.72 Gate.power :cartridge,:OFF @07:05:26.29 Mounted archive Cartridge @07:05:26.35 BACsampler with {source: :skimmer} @07:05:26.48 GoodNews.email "BACsampler with {source: :skimmer} filtering up to 100 ml",Subject: "Sampling archiveNull_bac cartridge in slot 15" @07:05:26.86 Preparing to filter cartridge 15 @07:05:27.21 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:05:32.52 upsync @07:05:39.86 Intake Head Pressure tare is 3% of full scale at 24C (drifted 2%) @07:05:39.88 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @07:05:39.97 PhaseSeparator.in :filling @07:05:40.06 PhaseSeparator.hold @07:05:40.17 Gate.power :skimmer,:ON @07:05:55.20 Gate.power :skimmer,:OFF @07:05:55.59 Gate.power :skimmer,:ON @07:06:10.62 Gate.power :skimmer,:OFF @07:06:11.00 Gate.power :skimmer,:ON @07:06:26.03 Gate.power :skimmer,:OFF @07:06:26.42 Gate.power :skimmer,:ON @07:06:41.48 Gate.power :skimmer,:OFF @07:06:41.86 Gate.power :skimmer,:ON @07:06:56.90 Gate.power :skimmer,:OFF @07:06:57.27 Gate.power :skimmer,:ON @07:07:12.31 Gate.power :skimmer,:OFF @07:07:12.69 Gate.power :skimmer,:ON @07:07:27.72 Gate.power :skimmer,:OFF @07:07:28.11 Gate.power :skimmer,:ON @07:07:40.24 PhaseSeparator.stop @07:07:40.35 Cartridge::Sampler::HeadPressureLow in FILTERING -- sufficient, but only reached 0.63psi in 2:01 @07:07:40.52 Priming sample loop w/150ml, bypass w/50ml @07:07:40.54 SamplePump.setPosition! 0ml @07:07:40.63 Bypass.open @07:07:42.15 Intake.open @07:07:42.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:07:45.56 Vacuum tare is 52% of full scale at 24C (drifted 0%) @07:07:45.85 Abs.in :default @07:07:45.94 Abs.hold @07:07:46.06 PhaseSeparator.in :full @07:07:46.14 PhaseSeparator.hold @07:07:46.44 Waiting for skimmer to finish priming... @07:07:46.48 delayUntil "07:08:58.13" @07:08:58.21 SamplePump.in :start @07:08:58.32 SamplePump.seek 50ml,3:50 @07:08:58.89 SamplePump.configure :prime @07:09:03.65 -> BACsampler.goalVolume=1000 1000 @07:09:59.75 Abs.stop @07:09:59.84 PhaseSeparator.stop @07:09:59.93 SamplePump.setPosition! 0ml @07:10:00.03 Exhaust.open @07:10:01.39 Bypass.close @07:10:02.94 Abs.in :default @07:10:03.05 Abs.hold @07:10:03.20 PhaseSeparator.hold @07:10:03.61 SamplePump.in :start @07:10:03.71 SamplePump.seek 150ml,10:30 @07:10:04.29 SamplePump.configure :prime @07:13:07.13 Abs.stop @07:13:07.23 PhaseSeparator.stop @07:13:07.34 Bypass.open @07:13:08.02 Intake.close @07:13:08.81 Toroid.in :hold @07:13:08.92 Toroid.seek :crack @07:13:10.50 Toroid.seek :cartridge @07:13:11.65 Toroid.in :default @07:13:11.76 Toroid.seek :clear @07:13:13.85 Pressurizing cartridge to 26psi above 13.9psia @07:13:13.87 SamplePump.setPosition! 0ml @07:13:13.98 Intake.open @07:13:14.68 Bypass.close @07:13:15.39 Exhaust.close @07:13:16.16 Abs.in :default @07:13:16.25 Abs.hold @07:13:16.37 PhaseSeparator.hold @07:13:17.28 SamplePump.in :charge @07:13:17.49 SamplePump.seek 2.3ml,42 seconds @07:13:32.76 Abs.stop @07:13:32.87 PhaseSeparator.stop @07:13:32.96 Intake.close @07:13:33.77 Pressurized cartridge with 1.048ml to 26.1psi @07:13:33.80 Isolated @36.4psia (22.5psi) @07:13:33.82 Verifying cartridge holds at least 0.65..0.85 of 36.4psia @07:13:33.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:13:38.61 Sampling 100.0ml @07:13:38.63 SamplePump.setPosition! 0ml @07:13:38.75 Exhaust.open @07:13:39.41 Intake.open @07:13:40.36 Abs.in :default @07:13:40.49 Abs.hold @07:13:40.61 PhaseSeparator.hold @07:13:41.00 SamplePump.in :start @07:13:41.11 SamplePump.seek 100ml,8:50 @07:13:41.69 SamplePump.configure :sample @07:13:44.10 Cartridge 15 filtering 2/100ml at 0.40ml/s with 23.5psi vacuum @07:14:44.18 Cartridge 15 filtering 31/100ml at 0.48ml/s with 23.9psi vacuum @07:15:44.16 Cartridge 15 filtering 49/100ml at 0.30ml/s with 23.8psi vacuum @07:16:44.16 Cartridge 15 filtering 55/100ml at 0.11ml/s with 22.6psi vacuum @07:16:44.22 SamplePump.stop @07:16:44.35 Intake.close @07:16:44.46 Exhaust.close @07:16:44.78 Toroid.in :hold @07:16:44.89 Toroid.seek :bypass @07:16:46.57 Toroid.in :default @07:16:46.67 Toroid.seek :clear @07:16:48.30 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @07:16:48.33 Abs.stop @07:16:48.47 PhaseSeparator.stop @07:16:48.75 Sampled 55.5ml @07:16:49.32 GoodNews.email "After filtering 55.5ml",Subject: "Processing archiveNull_bac cartridge in slot 15" @07:16:50.63 Plunge.seek :reagent @07:16:51.51 Sample loop closed. Ready to Deliver Reagents @07:16:51.72 Selecting Cartridge 1 /home/esp/esp2/lib/thread.rb:984: [BUG] Segmentation fault ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] @07:19:13.74PDT30-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @07:19:15.51 >BREAK< ESP3 Gateway v1.43 for core bus @07:19:15.54 >BREAK< ESP3 Gateway v1.43 for cartridge bus @07:19:19.31 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @07:19:20.01 Sea pressure (Abs) tare is 6% of full scale @07:19:27.97 Polling Can every 2:00 @07:19:28.03 Waiting for espclients... @07:19:28.89 Session Begun @07:19:47.68 -> ESP.ready! @07:19:47.88 Gate.power :sampler,:OFF,limit to 3500mA @07:19:47.90 Gate.power :skimmer,:OFF,limit to 3500mA @07:19:47.94 Gate.power :analytic1,:ON,limit to 4000mA @07:19:48.70 Plunge.configure :default @07:19:48.80 Plunge.hold @07:19:49.37 Plunge.configure :default @07:19:49.57 SamplePump.configure :default @07:19:49.76 Toroid.configure :default @07:19:49.86 Toroid.hold @07:19:50.23 Toroid.configure :default @07:19:50.44 Carousel.configure :default @07:19:50.54 Carousel.hold @07:19:50.66 Carousel.configure :default @07:19:50.83 Abs.configure :default @07:19:51.00 PhaseSeparator.configure :default @07:19:51.18 Archive.configure :default @07:19:51.37 Air.configure :default @07:19:51.46 Air.hold @07:19:51.57 Air.configure :default @07:19:51.98 Lysis1.configure :default @07:19:52.07 Lysis1.hold @07:19:52.17 Lysis1.configure :default @07:19:52.32 Lysis2.configure :default @07:19:52.53 PinPort.configure :default @07:19:52.62 All elf controllers running firmware version 4.92 @07:19:53.49 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @07:19:53.52 AnalyticModule::Unknown in ben -- SPR @07:19:54.28 Toroid.seek :clear @07:19:54.54 Carousel.in :default @07:19:54.68 Carousel.home.seek 15 @07:19:55.11 Carousel.reconfigure :locked @07:19:55.30 Powering cartridge bus @07:19:58.00 Gate.power :cartridge,:OFF @07:19:58.57 Mounted archive Cartridge Plunge = Plunge Valve (PV) at reagentLG Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 55.51ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 15 Abs = Absolute Pressure (Leak) Sensor @20psia PhaseSeparator = Intake Head Pressure @0.10psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) full Lysis1 = Lysis Syringe #1 (LyS1) locked Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @07:20:26.26 -> slots {1 => { Type: :archiveNull_bac, State: :dry}, 15 => { Type: :archiveNull_bac, State: :processed, filtered: 55.5}, 25 => { Type: :archiveNull_bac, State: :processed, filtered: 100}, 35 => { Type: :archiveNull_bac, State: :processed, filtered: 61.6}, 45 => { Type: :archiveNull_bac, State: :processed, filtered: 47.9}} @07:21:51.06 -> scum;fire archiveNull_bac # @07:21:52.70 Toroid.seek :clear @07:21:52.92 Carousel.in :default @07:21:53.07 Carousel.home.seek 15 @07:21:53.50 Carousel.reconfigure :locked @07:21:53.68 Powering cartridge bus @07:21:56.38 Gate.power :cartridge,:OFF @07:21:56.97 Mounted archive Cartridge @07:21:57.06 Selecting Cartridge 1 @07:21:57.32 Carousel.in :default @07:21:57.49 Carousel.home.seek 15 @07:21:57.94 Air.in :unlocking @07:21:58.11 Air.seek :clear @07:21:59.17 Lysis1.in :unlocking @07:21:59.31 Lysis1.seek :clear @07:21:59.49 Plunge.in :unlocking @07:21:59.57 Plunge.seek :clear @07:22:01.01 Carousel.seek 1 @07:22:04.24 Lysis1.in :fast @07:22:04.33 Lysis1.seek :locked @07:22:07.00 Plunge.in :default @07:22:07.14 Plunge.seek :locked @07:22:07.31 Air.in :fast @07:22:07.38 Air.seek :locked @07:22:08.41 Carousel.reconfigure :locked @07:22:08.62 Powering cartridge bus @07:22:11.31 Gate.power :cartridge,:OFF @07:22:11.88 Mounted archive Cartridge @07:22:11.96 BACsampler with {source: :skimmer} @07:22:12.07 GoodNews.email "BACsampler with {source: :skimmer} filtering up to 1000 ml",Subject: "Sampling archiveNull_bac cartridge in slot 1" @07:22:12.43 Preparing to filter cartridge 1 @07:22:12.77 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:22:18.12 upsync @07:22:20.92 Intake Head Pressure tare is 3% of full scale at 25C (drifted 2%) @07:22:20.94 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @07:22:21.01 PhaseSeparator.in :filling @07:22:21.09 PhaseSeparator.hold @07:22:21.29 Gate.power :skimmer,:ON @07:22:36.32 Gate.power :skimmer,:OFF @07:22:36.70 Gate.power :skimmer,:ON @07:22:51.73 Gate.power :skimmer,:OFF @07:22:52.11 Gate.power :skimmer,:ON @07:23:07.14 Gate.power :skimmer,:OFF @07:23:07.52 Gate.power :skimmer,:ON @07:23:22.56 Gate.power :skimmer,:OFF @07:23:22.94 Gate.power :skimmer,:ON @07:23:38.00 Gate.power :skimmer,:OFF @07:23:38.38 Gate.power :skimmer,:ON @07:23:53.42 Gate.power :skimmer,:OFF @07:23:53.80 Gate.power :skimmer,:ON @07:24:08.86 Gate.power :skimmer,:OFF @07:24:09.24 Gate.power :skimmer,:ON @07:24:11.56 -> SC.status.deltaPress 7763 @07:24:17.22 -> Head Intake Head Pressure @0.63psi @07:24:19.58 -> Head Intake Head Pressure @0.67psi @07:24:21.36 PhaseSeparator.stop @07:24:21.48 Cartridge::Sampler::HeadPressureLow in FILTERING -- sufficient, but only reached 0.63psi in 2:01 @07:24:21.60 Priming sample loop w/150ml, bypass w/50ml @07:24:21.62 SamplePump.setPosition! 0ml @07:24:21.73 Exhaust.close @07:24:21.85 Bypass.open @07:24:23.33 Intake.open @07:24:24.10 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:24:26.73 Vacuum tare is 52% of full scale at 25C (drifted 1%) @07:24:26.93 Abs.in :default @07:24:27.01 Abs.hold @07:24:27.14 PhaseSeparator.in :full @07:24:27.23 PhaseSeparator.hold @07:24:27.49 Waiting for skimmer to finish priming... @07:24:27.52 delayUntil "07:25:39.26" @07:25:39.32 SamplePump.in :start @07:25:39.43 SamplePump.seek 50ml,3:50 @07:25:40.02 SamplePump.configure :prime @07:26:40.82 Abs.stop @07:26:40.91 PhaseSeparator.stop @07:26:40.99 SamplePump.setPosition! 0ml @07:26:41.07 Exhaust.open @07:26:42.47 Bypass.close @07:26:44.00 Abs.in :default @07:26:44.10 Abs.hold @07:26:44.21 PhaseSeparator.hold @07:26:44.60 SamplePump.in :start @07:26:44.71 SamplePump.seek 150ml,10:30 @07:26:45.30 SamplePump.configure :prime @07:28:50.25 -> Head Intake Head Pressure @0.58psi @07:29:48.10 Abs.stop @07:29:48.19 PhaseSeparator.stop @07:29:48.32 Bypass.open @07:29:49.01 Intake.close @07:29:49.79 Toroid.in :hold @07:29:49.89 Toroid.seek :crack @07:29:51.92 Toroid.seek :cartridge @07:29:53.07 Toroid.in :default @07:29:53.16 Toroid.seek :clear @07:29:55.32 Pressurizing cartridge to 26psi above 13.9psia @07:29:55.34 SamplePump.setPosition! 0ml @07:29:55.42 Intake.open @07:29:56.12 Bypass.close @07:29:56.80 Exhaust.close @07:29:57.61 Abs.in :default @07:29:57.69 Abs.hold @07:29:57.81 PhaseSeparator.hold @07:29:58.09 SamplePump.in :charge @07:29:58.20 SamplePump.seek 2.3ml,42 seconds @07:30:00.32 -> Head Intake Head Pressure @1.32psi @07:30:13.67 Abs.stop @07:30:13.77 PhaseSeparator.stop @07:30:13.87 Intake.close @07:30:14.64 Pressurized cartridge with 1.076ml to 26.4psi @07:30:14.67 Isolated @38.3psia (24.5psi) @07:30:14.71 Verifying cartridge holds at least 0.65..0.85 of 38.3psia @07:30:14.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:30:19.46 Sampling 1000.0ml @07:30:19.49 SamplePump.setPosition! 0ml @07:30:19.62 Exhaust.open @07:30:20.26 Intake.open @07:30:21.15 Abs.in :default @07:30:21.24 Abs.hold @07:30:21.35 PhaseSeparator.hold @07:30:21.74 SamplePump.in :start @07:30:21.85 SamplePump.seek 1000ml,1:23:50 @07:30:22.44 SamplePump.configure :sample @07:30:24.83 Cartridge 1 filtering 2/1000ml at 0.52ml/s with 24.1psi vacuum @07:31:24.89 Cartridge 1 filtering 12/1000ml at 0.17ml/s with 28.3psi vacuum @07:31:24.96 SamplePump.stop @07:31:25.07 Intake.close @07:31:25.18 Exhaust.close @07:31:25.45 Toroid.in :hold @07:31:25.55 Toroid.seek :bypass @07:31:27.24 Toroid.in :default @07:31:27.34 Toroid.seek :clear @07:31:29.01 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @07:31:29.60 Abs.stop @07:31:29.72 PhaseSeparator.stop @07:31:29.98 Sampled 12.0ml @07:31:30.55 GoodNews.email "After filtering 12.0ml",Subject: "Processing archiveNull_bac cartridge in slot 1" @07:31:31.12 Plunge.seek :reagent @07:31:31.98 Sample loop closed. Ready to Deliver Reagents @07:31:32.37 Carousel.in :default @07:31:32.60 Carousel.home.seek 1 @07:31:33.04 Plunge.seek :locked @07:31:34.46 Air.in :unlocking @07:31:34.77 Air.seek :clear @07:31:34.92 Lysis1.in :unlocking @07:31:35.13 Lysis1.seek :clear @07:31:36.68 Gate.power :analytic1,:OFF @07:31:36.74 upsync @07:31:36.79 Gate.power :skimmer,:OFF @07:31:36.83 Gate.power :motor,:OFF @07:31:36.85 Gate.power :core,:OFF @07:31:36.92 Gate.power :main,:OFF @07:31:36.97 Safely stopped and ready to power off @07:32:41.71 -> can Can@07:32:38, 24.6C, 46% humidity, 14.2psia, 14.872V, 0.083A, 1.965Ah, 1.24W @07:33:52.24 -> can Can@07:33:52, 24.5C, 46% humidity, 14.2psia, 14.872V, 0.083A, 1.967Ah, 1.24W @07:33:54.53 -> can Can@07:33:52, 24.5C, 46% humidity, 14.2psia, 14.872V, 0.083A, 1.967Ah, 1.24W @07:35:01.13 -> Power.skimmer :on;loop{puts Head;Delay.sleep .5} @07:35:01.16 Gate.power :skimmer,:ON @07:35:01.24 BusOff in ben -- core bus was not powered while reading Intake Head Pressure's status @07:35:06.66 -> ESP.ready! @07:35:06.75 Gate.power :skimmer,:OFF @07:35:06.77 Gate.power :sampler,:OFF,limit to 3500mA @07:35:06.83 Gate.power :skimmer,:OFF,limit to 3500mA @07:35:06.86 Gate.power :main,:ON,limit to 5000mA @07:35:06.90 Gate.power :analytic1,:ON,limit to 4000mA @07:35:07.01 Powering core bus @07:35:09.79 Plunge.configure :default @07:35:09.91 SamplePump.configure :default @07:35:10.02 Toroid.configure :default @07:35:10.13 Carousel.configure :default @07:35:10.24 Abs.configure :default @07:35:10.35 PhaseSeparator.configure :full @07:35:10.51 Archive.configure :default @07:35:10.62 Air.configure :default @07:35:10.71 Lysis1.configure :default @07:35:11.05 Lysis2.configure :default @07:35:11.17 PinPort.configure :default @07:35:11.25 All elf controllers running firmware version 4.92 @07:35:11.29 Gate.power :motor,:ON,limit to 5000mA @07:35:14.27 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @07:35:14.30 AnalyticModule::Unknown in ben -- SPR @07:35:14.75 Plunge.home.jog 0 @07:35:15.33 Lysis2.home.jog 0 @07:35:15.94 Air.home.jog 0 @07:35:16.00 Carousel.home.jog 0 @07:35:16.06 Lysis1.home.jog 0 @07:35:16.11 Toroid.home.jog 0 @07:35:16.16 PinPort.home.jog 0 @07:35:16.34 Archive.home.jog 0 @07:35:17.15 Plunge.in :homing @07:35:17.49 Plunge.jog 1610 @07:35:17.71 Lysis2.in :homing @07:35:17.79 Lysis1.in :homing @07:35:17.85 Archive.in :homing @07:35:17.91 PinPort.in :homing @07:35:17.95 Air.in :homing @07:35:18.05 Toroid.in :homing @07:35:18.20 Lysis2.jog 1404 @07:35:18.38 Lysis1.jog 1392 @07:35:18.52 PinPort.jog -111 @07:35:18.57 Air.jog 1363 @07:35:18.62 Archive.jog 1391 @07:35:18.66 Toroid.jog 634 @07:35:19.11 Plunge.setRawPosition! 50 @07:35:19.14 PinPort.setRawPosition! 0 @07:35:19.29 PinPort.seek :home @07:35:19.33 Plunge.seek :home @07:35:19.58 PinPort.in :default @07:35:19.68 Lysis2.setRawPosition! 50 @07:35:19.79 Lysis2.seek :home @07:35:19.93 Plunge.in :default @07:35:20.07 Air.setRawPosition! 50 @07:35:20.11 Archive.setRawPosition! 50 @07:35:20.15 Lysis1.setRawPosition! 50 @07:35:20.31 Air.seek :home @07:35:20.43 Archive.seek :home @07:35:20.50 Lysis1.seek :home @07:35:20.78 Lysis2.in :default @07:35:20.94 Air.in :default @07:35:20.97 Archive.in :default @07:35:21.18 Toroid.setRawPosition! 0 @07:35:21.21 Lysis1.in :default @07:35:21.36 Toroid.seek :home @07:35:21.51 Toroid.in :default @07:35:21.64 Toroid.seek :clear @07:35:23.38 Carousel.in :default @07:35:23.54 Carousel.home.seek 1 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 1 Abs = Absolute Pressure (Leak) Sensor @19psia PhaseSeparator = Intake Head Pressure @0.03psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @07:35:29.92 -> Power.skimmer :on;loop{puts Head;Delay.sleep .5} @07:35:29.95 Gate.power :skimmer,:ON @07:35:30.08 Slide::Error in ben -- Intake Head Pressure Not yet Homed! @07:35:42.68 -> Head Intake Head Pressure @1.36psi @07:35:54.21 -> Power.skimmer :off @07:35:54.26 Gate.power :skimmer,:OFF false @07:36:33.28 -> Power.skimmer :on @07:36:33.30 Gate.power :skimmer,:ON true @07:36:34.40 -> Power.skimmer :on @07:36:34.45 Gate.power :skimmer,:ON true @07:36:37.32 -> Head Intake Head Pressure @2.19psi @07:36:38.30 -> Head Intake Head Pressure @1.36psi @07:36:39.32 -> Head Intake Head Pressure @1.38psi @07:36:40.10 -> Head Intake Head Pressure @1.36psi @07:36:40.78 -> Head Intake Head Pressure @1.40psi @07:36:48.87 -> Power.skimmer off @07:36:48.89 Gate.power :skimmer,:OFF false @07:36:56.52 -> Head Intake Head Pressure @0.04psi @07:36:58.00 -> Head Intake Head Pressure @0.02psi @07:37:10.23 -> Power.skimmer :on;Head @07:37:10.26 Gate.power :skimmer,:ON Intake Head Pressure @-0.01psi @07:37:13.58 -> Head Intake Head Pressure @1.81psi @07:37:15.31 -> Head Intake Head Pressure @1.35psi @07:37:16.70 -> Head Intake Head Pressure @1.28psi @07:37:17.93 -> Head Intake Head Pressure @1.33psi @07:37:19.29 -> Head Intake Head Pressure @1.28psi @07:37:20.70 -> Head Intake Head Pressure @1.31psi @07:38:09.64 -> Power.skimmer :off @07:38:09.66 Gate.power :skimmer,:OFF false @07:38:30.22 -> Power.skimmer :on;Head @07:38:30.25 Gate.power :skimmer,:ON Intake Head Pressure @-0.00psi @07:38:31.38 -> Head Intake Head Pressure @0.05psi @07:38:33.05 -> Head Intake Head Pressure @0.04psi @07:38:34.29 -> Head Intake Head Pressure @0.03psi @07:38:35.97 -> Head Intake Head Pressure @0.03psi @07:38:36.80 -> Head Intake Head Pressure @0.83psi @07:38:37.57 -> Head Intake Head Pressure @0.85psi @07:38:38.22 -> Head Intake Head Pressure @0.83psi @07:38:38.80 -> Head Intake Head Pressure @0.84psi @07:38:39.41 -> Head Intake Head Pressure @0.86psi @07:38:39.97 -> Head Intake Head Pressure @0.84psi @07:38:40.50 -> Head Intake Head Pressure @0.83psi @07:38:41.01 -> Head Intake Head Pressure @0.85psi @07:38:41.55 -> Head Intake Head Pressure @0.82psi @07:38:42.13 -> Head Intake Head Pressure @0.87psi @07:38:42.77 -> Head Intake Head Pressure @0.85psi @07:38:43.33 -> Head Intake Head Pressure @0.84psi @07:38:43.93 -> Head Intake Head Pressure @0.83psi @07:39:02.09 -> Power.sampler :off @07:39:02.11 Gate.power :sampler,:OFF false @07:39:09.87 -> Power.skimmer :off @07:39:09.89 Gate.power :skimmer,:OFF false @07:50:37.91 -> SC.coast @07:50:37.93 Carousel.coast Storage Carousel (SC) at 1 @07:55:31.99 -> ESP.shutdown @07:55:32.21 Gate.power :analytic1,:OFF @07:55:32.23 Gate.power :motor,:OFF @07:55:32.27 Gate.power :core,:OFF @07:55:32.31 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @08:10:27.95PDT30-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @08:10:31.91 >BREAK< ESP3 Gateway v1.43 for core bus @08:10:31.94 >BREAK< ESP3 Gateway v1.43 for cartridge bus @08:10:36.18 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @08:10:37.03 Sea pressure (Abs) tare is 6% of full scale @08:10:44.60 Polling Can every 2:00 @08:10:44.68 Waiting for espclients... @08:10:45.29 Session Begun @08:11:05.39 -> ESP.ready! @08:11:05.45 Gate.power :sampler,:OFF,limit to 3500mA @08:11:05.51 Gate.power :skimmer,:OFF,limit to 3500mA @08:11:05.53 Gate.power :main,:ON,limit to 5000mA @08:11:05.56 Gate.power :analytic1,:ON,limit to 4000mA @08:11:05.79 Powering core bus @08:11:08.63 Plunge.configure :default @08:11:08.75 SamplePump.configure :default @08:11:09.39 Toroid.configure :default @08:11:09.78 Carousel.configure :default @08:11:09.89 Abs.configure :default @08:11:10.01 PhaseSeparator.configure :default @08:11:10.12 Archive.configure :default @08:11:10.24 Air.configure :default @08:11:10.36 Lysis1.configure :default @08:11:10.47 Lysis2.configure :default @08:11:10.60 PinPort.configure :default @08:11:10.69 All elf controllers running firmware version 4.92 @08:11:10.74 Gate.power :motor,:ON,limit to 5000mA @08:11:12.96 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @08:11:13.00 AnalyticModule::Unknown in na -- SPR @08:11:13.47 Plunge.home.jog 0 @08:11:14.06 Air.home.jog 0 @08:11:14.13 Archive.home.jog 0 @08:11:14.17 Lysis2.home.jog 0 @08:11:14.22 Toroid.home.jog 0 @08:11:14.35 Lysis1.home.jog 0 @08:11:14.40 PinPort.home.jog 0 @08:11:14.47 Carousel.home.jog 0 @08:11:15.15 Plunge.in :homing @08:11:15.70 PinPort.in :homing @08:11:15.77 Plunge.jog 158 @08:11:15.82 Lysis1.in :homing @08:11:15.87 Archive.in :homing @08:11:15.92 Toroid.in :homing @08:11:15.97 Lysis2.in :homing @08:11:16.11 Air.in :homing @08:11:16.36 PinPort.jog -111 @08:11:16.47 Lysis1.jog 1395 @08:11:16.53 Archive.jog 1389 @08:11:16.60 Lysis2.jog 1402 @08:11:16.64 Toroid.jog 621 @08:11:16.76 Air.jog 1337 @08:11:17.22 Plunge.setRawPosition! 50 @08:11:17.25 PinPort.setRawPosition! 0 @08:11:17.37 Plunge.seek :home @08:11:17.48 PinPort.seek :home @08:11:17.67 PinPort.in :default @08:11:17.79 Plunge.in :default @08:11:18.05 Lysis1.setRawPosition! 50 @08:11:18.17 Lysis1.seek :home @08:11:18.38 Lysis2.setRawPosition! 50 @08:11:18.41 Air.setRawPosition! 50 @08:11:18.57 Archive.setRawPosition! 50 @08:11:18.69 Lysis2.seek :home @08:11:18.78 Air.seek :home @08:11:18.83 Archive.seek :home @08:11:19.22 Lysis1.in :default @08:11:19.27 Toroid.setRawPosition! 0 @08:11:19.44 Toroid.seek :home @08:11:19.61 Lysis2.in :default @08:11:19.71 Air.in :default @08:11:19.76 Archive.in :default @08:11:19.81 Toroid.in :default @08:11:20.31 Toroid.seek :clear @08:11:22.01 Carousel.in :default @08:11:22.17 Carousel.home.seek 54 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @0.15psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @08:12:47.32 -> ESP.ready! @08:12:48.26 Toroid.seek :clear @08:12:48.49 Carousel.in :default @08:12:48.64 Carousel.home.seek 54 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @0.12psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @08:12:52.22 -> SC.to 25 @08:12:52.31 Carousel.seek 25 Storage Carousel (SC) at 25 @08:13:42.74 Session Begun @08:13:56.52 -> SC Storage Carousel (SC) at 25 @08:14:10.69 -> BACsampler.filter 1000 @08:14:10.86 Cartridge::Error in preston -- Not Locked @08:14:18.03 -> to 25 @08:14:18.44 Carousel.seek 25 @08:14:18.75 Lysis1.in :fast @08:14:18.87 Lysis1.seek :locked @08:14:21.55 Plunge.seek :locked @08:14:21.79 Air.in :fast @08:14:21.86 Air.seek :locked @08:14:22.91 Carousel.reconfigure :locked @08:14:23.15 Powering cartridge bus @08:14:25.92 Gate.power :cartridge,:OFF @08:14:26.58 Mounted archive Cartridge :archiveNull_bac @08:14:28.95 -> BACsampler.filter 1000 @08:14:29.26 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @08:14:50.40 Intake Head Pressure tare is 3% of full scale at 20C (drifted 2%) @08:14:50.43 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @08:14:50.54 PhaseSeparator.in :filling @08:14:50.67 PhaseSeparator.hold @08:14:50.82 Gate.power :sampler,:ON @08:15:05.85 Gate.power :sampler,:OFF @08:15:06.29 Gate.power :sampler,:ON @08:15:08.58

Session Begun @08:15:12.00 -> Sampler.psi 2.01673545540109 @08:15:21.33 Gate.power :sampler,:OFF @08:15:21.72 Gate.power :sampler,:ON @08:15:36.76 Gate.power :sampler,:OFF @08:15:37.14 Gate.power :sampler,:ON @08:15:52.18 Gate.power :sampler,:OFF @08:15:52.57 Gate.power :sampler,:ON @08:16:07.62 Gate.power :sampler,:OFF @08:16:08.00 Gate.power :sampler,:ON @08:16:23.05 Gate.power :sampler,:OFF @08:16:23.43 Gate.power :sampler,:ON @08:16:27.90 Head pressure reached 0.91psi in 1:38 @08:16:28.09 Priming sample loop w/150ml, bypass w/50ml @08:16:28.12 SamplePump.setPosition! 0ml @08:16:28.35 Bypass.open @08:16:29.87 Intake.open @08:16:30.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:16:33.36 Vacuum tare is 52% of full scale at 20C (drifted 0%) @08:16:33.59 Abs.in :default @08:16:33.72 Abs.hold @08:16:33.86 PhaseSeparator.in :full @08:16:34.00 PhaseSeparator.hold @08:16:34.36 Waiting for sampler to finish priming... @08:16:34.43 delayUntil "08:17:53.45" @08:16:41.67

-> Sampler.psi -0.0519715312273377 @08:16:48.42 -> Sampler.psi -0.0565572545709472 @08:16:55.69 -> Sampler.psi -0.285843421750371 @08:17:53.54 SamplePump.in :start @08:17:53.69 SamplePump.seek 50ml,3:50 @08:17:54.24 SamplePump.configure :prime @08:17:57.62

-> Sampler.psi 2.16293284372597 @08:17:58.73 -> Sampler.psi 3.18554914934626 @08:18:02.04 -> Sampler.psi 4.25402268840242 @08:18:03.49 -> Sampler.psi -0.519715312273405 @08:18:07.05 -> Sampler.psi 2.52061926452589 @08:18:09.08 -> Sampler.psi 2.29133309734647 @08:18:12.47 -> Sampler.psi -0.671044182611809 @08:18:14.32 -> Sampler.psi -0.473858078837509 @08:18:15.97 -> Sampler.psi 0.301129166228975 @08:18:18.49 -> Sampler.psi 2.34636177746953 @08:18:20.82 -> Sampler.psi 1.59430314912098 @08:18:45.61 -> Sampler.psi 3.94677922438197 @08:18:48.49 -> Sampler.psi 1.99326108001321 @08:18:51.07 -> Sampler.psi 2.75449115504892 @08:18:53.18 -> Sampler.psi 2.52979071121308 @08:18:55.12 Abs.stop @08:18:55.26 PhaseSeparator.stop @08:18:55.36

-> Sampler.psi @08:18:55.45 SamplePump.setPosition! 0ml -0.533472482304148 @08:18:55.80 Exhaust.open @08:18:57.31 Bypass.close @08:18:58.94 Abs.in :default @08:18:59.08 Abs.hold @08:18:59.25 PhaseSeparator.hold @08:18:59.69 SamplePump.in :start @08:18:59.82 SamplePump.seek 150ml,10:30 @08:19:00.37 SamplePump.configure :prime @08:22:03.28 Abs.stop @08:22:03.40 PhaseSeparator.stop @08:22:03.60 Bypass.open @08:22:04.35 Intake.close @08:22:05.15 Toroid.in :hold @08:22:05.29 Toroid.seek :crack @08:22:06.91 Toroid.seek :cartridge @08:22:08.07 Toroid.in :default @08:22:08.20 Toroid.seek :clear @08:22:10.39 Pressurizing cartridge to 26psi above 15.1psia @08:22:10.42 SamplePump.setPosition! 0ml @08:22:10.59 Intake.open @08:22:11.28 Bypass.close @08:22:12.00 Exhaust.close @08:22:12.86 Abs.in :default @08:22:12.98 Abs.hold @08:22:13.13 PhaseSeparator.hold @08:22:13.46 SamplePump.in :charge @08:22:13.59 SamplePump.seek 2.3ml,42 seconds @08:22:24.20 Abs.stop @08:22:24.36 PhaseSeparator.stop @08:22:24.49 Intake.close @08:22:25.31 Pressurized cartridge with 0.499ml to 26.2psi @08:22:25.32 Isolated @40.1psia (25.1psi) @08:22:25.40 Verifying cartridge holds at least 0.65..0.85 of 40.1psia @08:22:25.55 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:22:30.27 Sampling 1000.0ml @08:22:30.28 SamplePump.setPosition! 0ml @08:22:30.46 Exhaust.open @08:22:31.18 Intake.open @08:22:32.21 Abs.in :default @08:22:32.37 Abs.hold @08:22:32.54 PhaseSeparator.hold @08:22:32.99 SamplePump.in :start @08:22:33.15 SamplePump.seek 1000ml,1:23:50 @08:22:33.68 SamplePump.configure :sample @08:22:36.10 Cartridge 25 filtering 1/1000ml at 0.44ml/s with 21.8psi vacuum @08:23:36.17 Cartridge 25 filtering 30/1000ml at 0.48ml/s with 25.7psi vacuum @08:24:36.15 Cartridge 25 filtering 41/1000ml at 0.18ml/s with 22.2psi vacuum @08:24:36.31 SamplePump.stop @08:24:36.45 Intake.close @08:24:36.52 Exhaust.close @08:24:36.94 Toroid.in :hold @08:24:37.08 Toroid.seek :bypass @08:24:38.81 Toroid.in :default @08:24:38.94 Toroid.seek :clear @08:24:40.64 Cartridge::Sampler::Stuffed in preston -- Cartridge clogged @08:24:40.66 Abs.stop @08:24:40.84 PhaseSeparator.stop @08:24:41.15 Sampled 41.4ml 41.3626149131767 @08:24:44.44 Gate.power :sampler,:OFF @08:28:29.05

-> BACsampler.prime 100, nil @08:28:29.22 Priming sample loop w/100ml @08:28:29.25 SamplePump.setPosition! 0ml @08:28:29.41 Bypass.open @08:28:31.05 Exhaust.open @08:28:32.49 Bypass.close @08:28:32.53

Intake.open @08:28:34.32 Abs.in :default @08:28:34.43 Abs.hold @08:28:34.57 PhaseSeparator.hold @08:28:35.00 SamplePump.stop @08:28:35.19 Cartridge::Sampler::LostHeadPressure in p -- Head Pressure Fell to 0.03psi @08:28:35.22 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @08:28:35.36 PhaseSeparator.in :filling @08:28:35.49 PhaseSeparator.hold @08:28:35.65 Gate.power :sampler,:ON @08:28:44.35 Head pressure reached 0.93psi in 10 seconds @08:28:44.37 Abs.hold @08:28:44.61 PhaseSeparator.in :full @08:28:45.32 PhaseSeparator.hold @08:28:45.73 Waiting for sampler to finish priming... @08:28:45.74 delayUntil "08:30:05.68" @08:30:05.77 SamplePump.in :start @08:30:05.90 SamplePump.seek 100ml,7:10 @08:30:06.46 SamplePump.configure :prime @08:31:10.90 Session Ended @08:31:30.30 -> loop {puts Sampler.psi} @08:31:39.18 UserAbort in preston -- User Abort!! @08:31:42.89

SamplePump.hold @08:31:43.21 Intake.close @08:31:43.29 Exhaust.close @08:31:44.17

Abs.stop @08:31:44.96 PhaseSeparator.stop @08:31:45.10 UserAbort in p -- Sample Pump (SP) seek to 100ml Aborted! @08:32:25.92 -> TV.cartridge @08:32:26.09 Toroid.in :hold @08:32:26.22 Toroid.seek :cartridge @08:32:28.14 Toroid.in :default @08:32:28.30 Toroid.seek :clear Toroid Valve (TV) clear @08:32:34.90 -> TV.bypass @08:32:35.08 Toroid.in :hold @08:32:35.19 Toroid.seek :bypass @08:32:36.89 Toroid.in :default @08:32:36.99 Toroid.seek :clear Toroid Valve (TV) clear @08:33:07.82 -> TV.bypass @08:33:07.98 Toroid.in :hold @08:33:08.09 Toroid.seek :bypass @08:33:09.76 Toroid.in :default @08:33:09.88 Toroid.seek :clear Toroid Valve (TV) clear @08:38:42.05 -> to 20 @08:38:42.40 Carousel.in :default @08:38:42.59 Carousel.home.seek 25 @08:38:43.11 Air.in :unlocking @08:38:43.30 Air.seek :clear @08:38:44.40 Lysis1.in :unlocking @08:38:44.58 Lysis1.seek :clear @08:38:44.75 Plunge.in :unlocking @08:38:44.87 Plunge.seek :clear @08:38:46.03

Carousel.seek 20 @08:38:47.44 Lysis1.in :fast @08:38:47.55 Lysis1.seek :locked @08:38:50.25 Plunge.in :default @08:38:50.42 Plunge.seek :locked @08:38:50.62 Air.in :fast @08:38:50.72 Air.seek :locked @08:38:51.75

Carousel.reconfigure :locked @08:38:51.96 Powering cartridge bus @08:38:54.68 Gate.power :cartridge,:OFF @08:38:55.28 Mounted archive Cartridge :archive_bac @08:39:30.61 -> BACsampler.primeVolume=BACsampler.bypassVolume=20 20 @08:39:44.83 -> BACsampler.filter 100 @08:39:45.12 Gate.power :sampler,:OFF @08:39:45.17 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @08:40:16.34 Intake Head Pressure tare is 3% of full scale at 21C (drifted 2%) @08:40:16.36 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @08:40:16.48 PhaseSeparator.in :filling @08:40:16.57 PhaseSeparator.hold @08:40:16.70 Gate.power :sampler,:ON @08:40:21.26 -> Head Intake Head Pressure @0.02psi @08:40:25.62

Head pressure reached 0.88psi in 10 seconds @08:40:25.75 Priming sample loop w/20ml, bypass w/20ml @08:40:25.79 SamplePump.setPosition! 0ml @08:40:25.92 Bypass.open @08:40:27.43 Intake.open @08:40:28.31 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:40:30.94 Vacuum tare is 52% of full scale at 21C (drifted 1%) @08:40:31.20 Abs.in :default @08:40:31.30 Abs.hold @08:40:31.46 PhaseSeparator.in :full @08:40:31.57 PhaseSeparator.hold @08:40:31.91 Waiting for sampler to finish priming... @08:40:31.93 delayUntil "08:41:46.73" @08:41:05.55 -> loop {puts Sampler.psi; puts Head; Delay.Sleep 1} @08:41:05.85 Slide::Error in preston -- Intake Head Pressure Not yet Homed! @08:41:18.98 -> Head Intake Head Pressure @0.93psi @08:41:21.72 -> loop {puts Sampler.psi; puts Head; Delay.Sleep 1} @08:41:22.00 Slide::Error in preston -- Intake Head Pressure Not yet Homed! @08:41:39.17 -> loop {puts Sampler.psi; puts Head.psia; Delay.Sleep 1} @08:41:39.31 NoMethodError in preston -- undefined method `psia' for PhaseSeparator:PressureSensor @08:41:46.81

SamplePump.in :start @08:41:46.94 SamplePump.seek 20ml,1:50 @08:41:47.50 SamplePump.configure :prime @08:41:55.97 -> loop {puts Sampler.psi; Delay.Sleep 1} @08:41:56.11 NoMethodError in preston -- undefined method `Sleep' for Delay:Class @08:42:03.01 -> loop {puts Sampler.ps} @08:42:03.04 NoMethodError in preston -- undefined method `ps' for Cartridge::Sampler:Class @08:42:06.61 -> loop {puts Sampler.psi} @08:42:11.83

Abs.stop @08:42:12.03 PhaseSeparator.stop @08:42:12.88 SamplePump.setPosition! 0ml @08:42:13.00 Exhaust.open @08:42:14.61 Bypass.close @08:42:16.62

Abs.in :default @08:42:16.83 Abs.hold @08:42:17.03 PhaseSeparator.hold @08:42:18.09 SamplePump.in :start @08:42:18.38 SamplePump.seek 20ml,1:50 @08:42:18.86 SamplePump.configure :prime @08:42:43.45

Abs.stop @08:42:43.63 PhaseSeparator.stop @08:42:43.86 Bypass.open @08:42:44.76 Intake.close @08:42:46.00 Toroid.in :hold @08:42:46.14 Toroid.seek :crack @08:42:48.07 Toroid.seek :cartridge @08:42:50.02 Toroid.in :default @08:42:50.22 Toroid.seek :clear @08:42:53.03 Pressurizing cartridge to 26psi above 15.0psia @08:42:53.14 SamplePump.setPosition! 0ml @08:42:53.27 Intake.open @08:42:54.02 Bypass.close @08:42:54.93 Exhaust.close @08:42:56.20 Abs.in :default @08:42:56.48 Abs.hold @08:42:56.76 PhaseSeparator.hold @08:42:57.50 SamplePump.in :charge @08:42:57.69 SamplePump.seek 2.3ml,42 seconds @08:43:06.10 Abs.stop @08:43:06.22 PhaseSeparator.stop @08:43:06.36 Intake.close @08:43:07.27 Pressurized cartridge with 0.290ml to 26.2psi @08:43:07.35 Isolated @39.6psia (24.6psi) @08:43:07.43 Verifying cartridge holds at least 0.65..0.85 of 39.6psia @08:43:07.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:43:12.36 Cartridge pressure fell 1% to 39.4psia @08:43:12.60 Sampling 100.0ml @08:43:12.68 SamplePump.setPosition! 0ml @08:43:12.83 Exhaust.open @08:43:13.64 Intake.open @08:43:15.20 Abs.in :default @08:43:15.36 Abs.hold @08:43:15.70 PhaseSeparator.hold @08:43:16.61 SamplePump.in :start @08:43:16.75 SamplePump.seek 100ml,8:50 @08:43:17.45 SamplePump.configure :sample @08:43:19.82 Cartridge 20 filtering 2/100ml at 0.72ml/s with 25.8psi vacuum @08:44:20.09 Cartridge 20 filtering 45/100ml at 0.72ml/s with 11.3psi vacuum 4.3m deep (21.0psia) @08:45:19.98 Cartridge 20 filtering 88/100ml at 0.72ml/s with 22.0psi vacuum 4.5m deep (21.3psia) @08:45:35.97

Abs.stop @08:45:36.14 PhaseSeparator.stop @08:45:36.48 Intake.close @08:45:36.57 Exhaust.close @08:45:36.97

Toroid.in :hold @08:45:37.14 Toroid.seek :bypass @08:45:38.95 Toroid.in :default @08:45:39.10 Toroid.seek :clear @08:45:40.94 Sampled 100.0ml 99.9997446373851 @08:45:41.50 UserAbort in preston -- User Abort!! @08:45:44.19 Gate.power :sampler,:OFF @08:46:01.95

-> BACsampler.filter 1000 @08:46:02.99 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @08:49:00.26 PressureError in p -- Pressure Failed to Stabilize @08:54:45.22 -> BACsampler.filter 1000 @08:54:45.50 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @08:55:57.63 Intake Head Pressure tare is 3% of full scale at 21C (drifted 2%) @08:55:57.64 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @08:55:57.74 PhaseSeparator.in :filling @08:55:57.82 PhaseSeparator.hold @08:55:57.96 Gate.power :sampler,:ON @08:56:06.62 Head pressure reached 1.01psi in 9 seconds @08:56:06.78 Toroid.in :hold @08:56:06.88 Toroid.seek :cartridge @08:56:08.77 Toroid.in :default @08:56:08.88 Toroid.seek :clear @08:56:10.73 Sampling 1000.0ml @08:56:10.75 SamplePump.setPosition! 0ml @08:56:10.88 Exhaust.open @08:56:12.31 Intake.open @08:56:13.28 Abs.in :default @08:56:13.38 Abs.hold @08:56:13.51 PhaseSeparator.in :full @08:56:13.62 PhaseSeparator.hold @08:56:13.97 Waiting for sampler to finish priming... @08:56:13.99 delayUntil "08:57:27.98" @08:57:28.06 SamplePump.in :start @08:57:28.19 SamplePump.seek 1000ml,1:23:50 @08:57:28.75 SamplePump.configure :sample @08:57:31.15 Cartridge 20 filtering 2/1000ml at 0.57ml/s with 20.0psi vacuum @08:58:31.22 Cartridge 20 filtering 45/1000ml at 0.72ml/s with 11.4psi vacuum @08:59:31.22 Cartridge 20 filtering 88/1000ml at 0.72ml/s with 19.7psi vacuum @09:00:31.21 Cartridge 20 filtering 132/1000ml at 0.72ml/s with 27.2psi vacuum @09:01:31.21 Cartridge 20 filtering 175/1000ml at 0.72ml/s with 24.7psi vacuum @09:02:31.22 Cartridge 20 filtering 218/1000ml at 0.72ml/s with 19.7psi vacuum @09:03:31.22 Cartridge 20 filtering 261/1000ml at 0.72ml/s with 27.7psi vacuum @09:04:31.21 Cartridge 20 filtering 304/1000ml at 0.72ml/s with 17.8psi vacuum @09:05:31.22 Cartridge 20 filtering 347/1000ml at 0.72ml/s with 28.8psi vacuum @09:06:31.22 Cartridge 20 filtering 391/1000ml at 0.72ml/s with 20.6psi vacuum 4.4m deep (21.3psia) @09:07:31.21 Cartridge 20 filtering 434/1000ml at 0.72ml/s with 18.4psi vacuum @09:08:31.21 Cartridge 20 filtering 477/1000ml at 0.72ml/s with 14.8psi vacuum 5.6m deep (22.9psia) @09:09:31.21 Cartridge 20 filtering 520/1000ml at 0.72ml/s with 24.8psi vacuum @09:10:31.22 Cartridge 20 filtering 562/1000ml at 0.71ml/s with 16.5psi vacuum @09:11:31.21 Cartridge 20 filtering 605/1000ml at 0.71ml/s with 9.5psi vacuum @09:12:31.22 Cartridge 20 filtering 648/1000ml at 0.71ml/s with 16.5psi vacuum @09:13:31.22 Cartridge 20 filtering 691/1000ml at 0.72ml/s with 25.9psi vacuum @09:14:31.21 Cartridge 20 filtering 734/1000ml at 0.72ml/s with 24.0psi vacuum @09:14:43.07 Session Begun @09:14:48.03 -> Power.sampler :off @09:14:48.05 Gate.power :sampler,:OFF false @09:14:48.46

SamplePump.stop @09:14:48.72 Cartridge::Sampler::LostHeadPressure in p -- Head Pressure Fell to -0.01psi @09:14:48.78 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @09:14:48.89 PhaseSeparator.in :filling @09:14:49.02 PhaseSeparator.hold @09:14:49.18 Gate.power :sampler,:ON @09:14:54.33 Head pressure reached 0.90psi in 6 seconds @09:14:54.40 Abs.hold @09:14:54.56 PhaseSeparator.in :full @09:14:54.69 PhaseSeparator.hold @09:14:55.12 SamplePump.in :start @09:14:55.25 SamplePump.seek 1000ml,21:35 @09:14:55.81 SamplePump.configure :sample @09:14:58.21 Cartridge 20 filtering 749/1000ml at 0.69ml/s with 26.4psi vacuum @09:15:15.86 -> Power.sampler :on @09:15:15.88 Gate.power :sampler,:ON true @09:15:58.29 Cartridge 20 filtering 792/1000ml at 0.71ml/s with 27.2psi vacuum @09:16:58.98 Cartridge 20 filtering 834/1000ml at 0.71ml/s with 24.6psi vacuum @09:17:51.99 -> SP Sample Pump (SP) at 872.34ml @09:17:58.30 Cartridge 20 filtering 877/1000ml at 0.71ml/s with 21.3psi vacuum @09:18:58.31 Cartridge 20 filtering 920/1000ml at 0.71ml/s with 26.5psi vacuum @09:19:58.31 Cartridge 20 filtering 962/1000ml at 0.72ml/s with 27.6psi vacuum @09:20:50.65

Abs.stop @09:20:50.76 PhaseSeparator.stop @09:20:50.97 Intake.close @09:20:51.04 Exhaust.close @09:20:51.39

Toroid.in :hold @09:20:51.51 Toroid.seek :bypass @09:20:53.21 Toroid.in :default @09:20:53.34 Toroid.seek :clear @09:20:55.03 Sampled 1000.0ml 999.999744637385 @09:20:58.20 Gate.power :sampler,:OFF @09:35:29.14 -> exit @09:35:29.16 Session Ended @09:35:32.18

-> exot :exot @09:35:33.55 -> exit @09:35:33.58 Session Ended @09:35:43.17 Session Begun @09:35:53.67 -> exit @09:35:53.69 Session Ended @10:27:04.33 -> ESP,ready! @10:27:04.36 SyntaxError in n -- compile error n:3: syntax error, unexpected $end, expecting tCOLON2 or '[' or '.' ESP,ready! ^ @10:27:10.99 -> ESP.ready! @10:27:11.80 Toroid.seek :clear @10:27:12.02 Carousel.in :default @10:27:12.15 Carousel.home.seek 20 @10:27:12.59 Carousel.reconfigure :locked @10:27:12.76 Powering cartridge bus @10:27:15.44 Gate.power :cartridge,:OFF @10:27:16.03 Mounted archive Cartridge Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 1000ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 20 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.00psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) full Lysis1 = Lysis Syringe #1 (LyS1) locked Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:29:06.88 -> slots [55,5,11,19]=>[:dry,:archiveNull_bac] {[55,19,11,5] => { Type: :archiveNull_bac, State: :dry}} @10:29:24.46 -> scum;fire arhciveNull_bac # @10:29:24.66 Cartridge::TypeUnknown in detached from n -- :arhciveNull_bac is not aRNAl_bac, aRNAl_phyto, aRNAl_zoo, archiveHiBiomass_bac, archiveNull_bac, archive_bac, archive_phyto, archive_zoo, blank_bac, blank_phyto, daSPR_bac, daSPR_phyto, daSPR_phyto9, daSPR_zoo, lyseHold_bac, lyseHold_phyto, lyseHold_zoo, lyseNull_bac, microSPR_bac, microSPR_phyto, microSPR_zoo or unknown @10:29:24.77 BadNews.email ":arhciveNull_bac is not aRNAl_bac, aRNAl_phyto, aRNAl_zoo, archiveHiBiomass_bac, archiveNull_bac, archive_bac, archive_phyto, archive_zoo, blank_bac, blank_phyto, daSPR_bac, daSPR_phyto, daSPR_phyto9, daSPR_zoo, lyseHold_bac, lyseHold_phyto, lyseHold_zoo, lyseNull_bac, microSPR_bac, microSPR_phyto, microSPR_zoo or unknown",Subject: "Cartridge::TypeUnknown in detached from n" @10:29:32.42 upsync @10:29:41.30 -> scum;fire archiveNull_bac # @10:29:42.43 Toroid.seek :clear @10:29:42.64 Carousel.in :default @10:29:42.81 Carousel.home.seek 20 @10:29:43.24 Carousel.reconfigure :locked @10:29:43.43 Powering cartridge bus @10:29:46.13 Gate.power :cartridge,:OFF @10:29:46.70 Mounted archive Cartridge @10:29:46.79 Selecting Cartridge 55 @10:29:47.05 Carousel.in :default @10:29:47.20 Carousel.home.seek 20 @10:29:47.65 Air.in :unlocking @10:29:47.82 Air.seek :clear @10:29:48.86 Lysis1.in :unlocking @10:29:49.03 Lysis1.seek :clear @10:29:49.12 Plunge.in :unlocking @10:29:49.27 Plunge.seek :clear @10:29:50.51 Carousel.seek 55 @10:29:53.62 -> Cmd.stop @10:29:53.64 Carousel.stop @10:29:53.91 Slide::Error in detached from n -- Storage Carousel (SC) aborted at 35 + 39 counts (sensed 35 + 42 counts) @10:29:53.94 Waiting to Resume from Checkpoint "Storage Carousel (SC) aborted at 35 + 39 counts (sensed 35 + 42 counts)" @10:29:53.98 BadNews.email "Storage Carousel (SC) aborted at 35 + 39 counts (sensed 35 + 42 counts)",Subject: "Slide::Error in detached from n" @10:29:54.19 Carousel.in :default @10:29:54.39 Carousel.home.seek 35 @10:29:54.95 Lysis1.in :fast @10:29:55.04 Lysis1.seek :locked @10:29:57.60 Plunge.in :default @10:29:57.72 Plunge.seek :locked @10:29:59.26 Lysis1.in :unlocking @10:29:59.49 Lysis1.seek :clear @10:30:00.10 upsync @10:30:01.03 Gate.power :analytic1,:OFF @10:30:01.05 Gate.power :motor,:OFF @10:30:01.11 Gate.power :core,:OFF @10:30:01.13 Gate.power :main,:OFF @10:30:01.16 Safely stopped and ready to power off :STOPPED @10:30:06.89 -> ESP.ready! @10:30:06.95 Gate.power :sampler,:OFF,limit to 3500mA @10:30:07.00 Gate.power :skimmer,:OFF,limit to 3500mA @10:30:07.07 Gate.power :main,:ON,limit to 5000mA @10:30:07.09 Gate.power :analytic1,:ON,limit to 4000mA @10:30:07.20 Powering core bus @10:30:09.99 Plunge.configure :default @10:30:10.10 SamplePump.configure :default @10:30:10.21 Toroid.configure :default @10:30:10.35 Carousel.configure :default @10:30:10.46 Abs.configure :default @10:30:10.57 PhaseSeparator.configure :full @10:30:10.67 Archive.configure :default @10:30:10.78 Air.configure :default @10:30:10.86 Lysis1.configure :default @10:30:11.21 Lysis2.configure :default @10:30:11.32 PinPort.configure :default @10:30:11.40 All elf controllers running firmware version 4.92 @10:30:11.43 Gate.power :motor,:ON,limit to 5000mA @10:30:14.48 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:30:14.51 AnalyticModule::Unknown in n -- SPR @10:30:14.79 Plunge.home.jog 0 @10:30:15.47 PinPort.home.jog 0 @10:30:15.55 Lysis2.home.jog 0 @10:30:15.60 Air.home.jog 0 @10:30:15.64 Carousel.home.jog 0 @10:30:15.74 Archive.home.jog 0 @10:30:15.79 Toroid.home.jog 0 @10:30:15.87 Lysis1.home.jog 0 @10:30:16.54 Plunge.in :homing @10:30:16.96 Plunge.jog 1612 @10:30:17.18 PinPort.in :homing @10:30:17.26 Lysis2.in :homing @10:30:17.30 Lysis1.in :homing @10:30:17.35 Archive.in :homing @10:30:17.40 Air.in :homing @10:30:17.46 Toroid.in :homing @10:30:17.69 PinPort.jog -108 @10:30:17.80 Lysis1.jog 1396 @10:30:17.87 Lysis2.jog 1407 @10:30:17.91 Air.jog 1362 @10:30:17.95 Archive.jog 1394 @10:30:18.08 Toroid.jog 609 @10:30:19.06 PinPort.setRawPosition! 0 @10:30:19.10 Plunge.setRawPosition! 50 @10:30:19.27 PinPort.seek :home @10:30:19.30 Plunge.seek :home @10:30:19.60 Lysis2.setRawPosition! 50 @10:30:19.66 Lysis1.setRawPosition! 50 @10:30:19.71 Air.setRawPosition! 50 @10:30:19.77 PinPort.in :default @10:30:19.79 Archive.setRawPosition! 50 @10:30:20.05 Lysis1.seek :home @10:30:20.14 Lysis2.seek :home @10:30:20.19 Air.seek :home @10:30:20.29 Archive.seek :home @10:30:20.65 Toroid.setRawPosition! 0 @10:30:20.68 Plunge.in :default @10:30:20.84 Toroid.seek :home @10:30:21.02 Lysis1.in :default @10:30:21.08 Air.in :default @10:30:21.12 Toroid.in :default @10:30:21.18 Archive.in :default @10:30:21.21 Lysis2.in :default @10:30:21.76 Toroid.seek :clear @10:30:23.49 Carousel.in :default @10:30:23.63 Carousel.home.seek 35 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 35 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @-0.02psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:30:25.46 -> slots {1 => { Type: :archiveNull_bac, State: :processed, filtered: 12}, [19,11,5] => { Type: :archiveNull_bac, State: :dry}, 15 => { Type: :archiveNull_bac, State: :processed, filtered: 55.5}, 20 => { Type: :archive_bac, State: :filtering}, 25 => { Type: :archiveNull_bac, State: :paused, filtered: 100}, 35 => { Type: :archiveNull_bac, State: :processed, filtered: 61.6}, 45 => { Type: :archiveNull_bac, State: :processed, filtered: 47.9}, 55 => { Type: :archiveNull_bac, State: :failedLoading}} @10:30:36.01 -> slots 20=>nil {20 => nil} @10:30:39.73 -> scum;fire archiveNull_bac # @10:30:40.77 Toroid.seek :clear @10:30:40.97 Carousel.in :default @10:30:41.11 Carousel.home.seek 35 @10:30:41.40 Selecting Cartridge 19 @10:30:41.74 Carousel.seek 19 @10:30:45.39 Lysis1.in :fast @10:30:45.47 Lysis1.seek :locked @10:30:48.14 Plunge.seek :locked @10:30:48.79 Air.in :fast @10:30:48.98 Air.seek :locked @10:30:49.99 Carousel.reconfigure :locked @10:30:50.20 Powering cartridge bus @10:30:50.65 -> Cmd.stop @10:30:50.85 Gate.power :cartridge,:OFF @10:30:50.96 Carousel.in :default @10:30:51.11 Carousel.home.seek 19 @10:30:51.24 Powering cartridge bus @10:30:51.48 Plunge.seek :locked @10:30:51.83 Air.in :unlocking @10:30:52.01 Air.seek :clear @10:30:52.09 Lysis1.in :unlocking @10:30:52.32 Lysis1.seek :clear @10:30:53.88 Gate.power :analytic1,:OFF @10:30:53.90 Gate.power :motor,:OFF @10:30:53.94 Gate.power :core,:OFF @10:30:53.98 Gate.power :main,:OFF @10:30:54.01 Safely stopped and ready to power off :STOPPED @10:30:54.92 Gate.power :cartridge,:OFF @10:30:54.97 Gate.power :sampler,:OFF,limit to 3500mA @10:30:55.01 -> slots @10:30:55.08 Gate.power :skimmer,:OFF,limit to 3500mA {1 => { Type: :archiveNull_bac, State: :processed, filtered: 12}, [19,11,5] => { Type: :archiveNull_bac, State: :dry}, 15 => { Type: :archiveNull_bac, State: :processed, filtered: 55.5}, 25 => { Type: :archiveNull_bac, State: :paused, filtered: 100}, 35 => { Type: :archiveNull_bac, State: :processed, filtered: 61.6}, 45 => { Type: :archiveNull_bac, State: :processed, filtered: 47.9}, 55 => { Type: :archiveNull_bac, State: :failedLoading}} @10:30:58.78 Gate.power :main,:ON,limit to 5000mA @10:30:58.82 Gate.power :analytic1,:ON,limit to 4000mA @10:30:58.93 Powering core bus @10:31:01.74 Plunge.configure :default @10:31:01.86 SamplePump.configure :default @10:31:01.98 Toroid.configure :default @10:31:02.11 Carousel.configure :default @10:31:02.25 Abs.configure :default @10:31:02.40 PhaseSeparator.configure :full @10:31:02.52 Archive.configure :default @10:31:02.91 Air.configure :default @10:31:03.01 Lysis1.configure :default @10:31:03.11 Lysis2.configure :default @10:31:03.22 PinPort.configure :default @10:31:03.32 All elf controllers running firmware version 4.92 @10:31:03.35 Gate.power :motor,:ON,limit to 5000mA @10:31:06.17 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:31:06.24 AnalyticModule::Unknown in detached from n#2 -- SPR @10:31:06.32 NoMethodError in detached from n#2 -- undefined method `>=' for nil:NilClass @10:31:06.34 Waiting to Resume from Checkpoint "Selecting Cartridge 19" @10:31:06.37 BadNews.email "undefined method `>=' for nil:NilClass",Subject: "NoMethodError in detached from n#2" @10:31:11.27 upsync @10:31:39.13 -> ESP.ready! @10:31:39.51 Plunge.home.jog 0 @10:31:40.36 Lysis2.home.jog 0 @10:31:40.42 Air.home.jog 0 @10:31:40.46 Carousel.home.jog 0 @10:31:40.52 Lysis1.home.jog 0 @10:31:40.56 Toroid.home.jog 0 @10:31:40.62 PinPort.home.jog 0 @10:31:40.68 Archive.home.jog 0 @10:31:41.49 Plunge.in :homing @10:31:41.76 Plunge.jog 1605 @10:31:42.01 PinPort.in :homing @10:31:42.06 Lysis2.in :homing @10:31:42.10 Air.in :homing @10:31:42.15 Toroid.in :homing @10:31:42.19 Lysis1.in :homing @10:31:42.27 Archive.in :homing @10:31:42.56 PinPort.jog -109 @10:31:42.61 Air.jog 1364 @10:31:42.66 Lysis2.jog 1405 @10:31:42.71 Lysis1.jog 1396 @10:31:42.76 Toroid.jog 592 @10:31:42.82 Archive.jog 1392 @10:31:43.32 PinPort.setRawPosition! 0 @10:31:43.35 Plunge.setRawPosition! 50 @10:31:43.50 PinPort.seek :home @10:31:43.53 Plunge.seek :home @10:31:43.77 PinPort.in :default @10:31:43.91 Plunge.in :default @10:31:44.08 Air.setRawPosition! 50 @10:31:44.16 Air.seek :home @10:31:44.33 Lysis1.setRawPosition! 50 @10:31:44.46 Lysis1.seek :home @10:31:44.61 Lysis2.setRawPosition! 50 @10:31:44.67 Air.in :default @10:31:44.70 Archive.setRawPosition! 50 @10:31:44.85 Lysis2.seek :home @10:31:45.03 Archive.seek :home @10:31:45.18 Lysis1.in :default @10:31:45.21 Toroid.setRawPosition! 0 @10:31:45.37 Toroid.seek :home @10:31:45.51 Archive.in :default @10:31:45.55 Toroid.in :default @10:31:45.60 Lysis2.in :default @10:31:46.06 Toroid.seek :clear @10:31:47.80 Carousel.in :default @10:31:47.95 Carousel.home.seek 19 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 19 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.01psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:31:50.32 -> slots {1 => { Type: :archiveNull_bac, State: :processed, filtered: 12}, [19,11,5] => { Type: :archiveNull_bac, State: :dry}, 15 => { Type: :archiveNull_bac, State: :processed, filtered: 55.5}, 25 => { Type: :archiveNull_bac, State: :paused, filtered: 100}, 35 => { Type: :archiveNull_bac, State: :processed, filtered: 61.6}, 45 => { Type: :archiveNull_bac, State: :processed, filtered: 47.9}, 55 => { Type: :archiveNull_bac, State: :failedLoading}} @10:32:20.56 -> slots 1..60=>nil {60..1 => nil} @10:32:43.74 -> slots [5,11,19,55]=>[:dry,:archiveNull_bac] {[55,19,11,5] => { Type: :archiveNull_bac, State: :dry}} @10:32:54.14 -> scum;fire archiveNull_bac # @10:32:55.16 Toroid.seek :clear @10:32:55.37 Carousel.in :default @10:32:55.51 Carousel.home.seek 19 @10:32:55.79 Selecting Cartridge 55 @10:32:56.12 Carousel.seek 55 @10:33:03.82 Lysis1.in :fast @10:33:03.91 Lysis1.seek :locked @10:33:06.56 Plunge.seek :locked @10:33:06.74 Air.in :fast @10:33:06.82 Air.seek :locked @10:33:07.82 Carousel.reconfigure :locked @10:33:08.00 Powering cartridge bus @10:33:10.68 Gate.power :cartridge,:OFF @10:33:11.26 Mounted archive Cartridge @10:33:11.34 BACsampler with {source: :skimmer} @10:33:11.36 GoodNews.email "BACsampler with {source: :skimmer} filtering up to 1000 ml",Subject: "Sampling archiveNull_bac cartridge in slot 55" @10:33:11.75 Preparing to filter cartridge 55 @10:33:12.09 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @10:33:16.47 upsync @10:33:21.22 Intake Head Pressure tare is 3% of full scale at 20C (drifted 2%) @10:33:21.24 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @10:33:21.32 PhaseSeparator.in :filling @10:33:21.39 PhaseSeparator.hold @10:33:21.52 Gate.power :skimmer,:ON @10:33:31.30 Head pressure reached 0.82psi in 10 seconds @10:33:31.41 Priming sample loop w/20ml, bypass w/20ml @10:33:31.43 SamplePump.setPosition! 0ml @10:33:31.54 Bypass.open @10:33:33.06 Intake.open @10:33:33.84 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:33:36.46 Vacuum tare is 52% of full scale at 20C (drifted 1%) @10:33:36.66 Abs.in :default @10:33:36.74 Abs.hold @10:33:36.84 PhaseSeparator.in :full @10:33:36.92 PhaseSeparator.hold @10:33:37.19 Waiting for skimmer to finish priming... @10:33:37.20 delayUntil "10:34:51.54" @10:34:51.61 SamplePump.in :start @10:34:51.70 SamplePump.seek 20ml,1:50 @10:34:52.29 SamplePump.configure :prime @10:35:16.49 Abs.stop @10:35:16.57 PhaseSeparator.stop @10:35:16.67 SamplePump.setPosition! 0ml @10:35:16.75 Exhaust.open @10:35:18.09 Bypass.close @10:35:19.66 Abs.in :default @10:35:19.76 Abs.hold @10:35:19.88 PhaseSeparator.hold @10:35:20.29 SamplePump.in :start @10:35:20.39 SamplePump.seek 20ml,1:50 @10:35:21.45 SamplePump.configure :prime @10:35:45.18 Abs.stop @10:35:45.28 PhaseSeparator.stop @10:35:45.41 Bypass.open @10:35:46.17 Intake.close @10:35:47.01 Toroid.in :hold @10:35:47.12 Toroid.seek :crack @10:35:48.71 Toroid.seek :cartridge @10:35:49.84 Toroid.in :default @10:35:49.93 Toroid.seek :clear @10:35:52.01 Pressurizing cartridge to 26psi above 14.9psia @10:35:52.03 SamplePump.setPosition! 0ml @10:35:52.13 Intake.open @10:35:52.81 Bypass.close @10:35:53.51 Exhaust.close @10:35:54.32 Abs.in :default @10:35:54.40 Abs.hold @10:35:54.51 PhaseSeparator.hold @10:35:54.80 SamplePump.in :charge @10:35:54.89 SamplePump.seek 2.3ml,42 seconds @10:36:09.12 Abs.stop @10:36:09.22 PhaseSeparator.stop @10:36:09.31 Intake.close @10:36:10.11 Pressurized cartridge with 0.908ml to 26.1psi @10:36:10.13 Isolated @39.3psia (24.5psi) @10:36:10.15 Verifying cartridge holds at least 0.65..0.85 of 39.3psia @10:36:10.22 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:36:14.88 Cartridge pressure fell 1% to 39.0psia @10:36:15.00 Sampling 1000.0ml @10:36:15.02 SamplePump.setPosition! 0ml @10:36:15.13 Exhaust.open @10:36:15.77 Intake.open @10:36:16.66 Abs.in :default @10:36:16.75 Abs.hold @10:36:16.86 PhaseSeparator.hold @10:36:17.25 SamplePump.in :start @10:36:17.37 SamplePump.seek 1000ml,1:23:50 @10:36:17.94 SamplePump.configure :sample @10:36:20.51 Cartridge 55 filtering 2/1000ml at 0.82ml/s with 15.2psi vacuum @10:37:20.57 Cartridge 55 filtering 43/1000ml at 0.69ml/s with 25.7psi vacuum @10:38:20.56 Cartridge 55 filtering 83/1000ml at 0.66ml/s with 22.1psi vacuum @10:39:20.56 Cartridge 55 filtering 122/1000ml at 0.66ml/s with 21.2psi vacuum @10:40:20.56 Cartridge 55 filtering 161/1000ml at 0.65ml/s with 25.6psi vacuum @10:41:20.57 Cartridge 55 filtering 200/1000ml at 0.65ml/s with 30.8psi vacuum @10:42:20.56 Cartridge 55 filtering 239/1000ml at 0.64ml/s with 12.2psi vacuum @10:43:20.56 Cartridge 55 filtering 277/1000ml at 0.64ml/s with 13.8psi vacuum 3.8m deep (20.3psia) @10:44:20.56 Cartridge 55 filtering 315/1000ml at 0.63ml/s with 25.7psi vacuum @10:45:20.57 Cartridge 55 filtering 353/1000ml at 0.63ml/s with 30.3psi vacuum @10:46:20.56 Cartridge 55 filtering 391/1000ml at 0.63ml/s with 24.7psi vacuum @10:47:20.56 Cartridge 55 filtering 429/1000ml at 0.63ml/s with 10.8psi vacuum @10:48:20.57 Cartridge 55 filtering 466/1000ml at 0.62ml/s with 20.6psi vacuum 5.0m deep (22.1psia) @10:49:20.56 Cartridge 55 filtering 503/1000ml at 0.62ml/s with 18.7psi vacuum 7.7m deep (26.1psia) @10:50:20.55 Cartridge 55 filtering 541/1000ml at 0.62ml/s with 24.3psi vacuum @10:51:20.57 Cartridge 55 filtering 578/1000ml at 0.62ml/s with 32.0psi vacuum @10:52:20.56 Cartridge 55 filtering 615/1000ml at 0.61ml/s with 28.6psi vacuum @10:53:20.56 Cartridge 55 filtering 651/1000ml at 0.61ml/s with 22.6psi vacuum @10:54:20.58 Cartridge 55 filtering 688/1000ml at 0.61ml/s with 30.1psi vacuum @10:55:20.56 Cartridge 55 filtering 724/1000ml at 0.61ml/s with 14.9psi vacuum @10:56:20.56 Cartridge 55 filtering 760/1000ml at 0.61ml/s with 15.5psi vacuum 9.8m deep (29.1psia) @10:57:20.57 Cartridge 55 filtering 796/1000ml at 0.60ml/s with 28.0psi vacuum @10:58:20.56 Cartridge 55 filtering 832/1000ml at 0.60ml/s with 20.8psi vacuum 6.4m deep (24.1psia) @10:59:20.56 Cartridge 55 filtering 868/1000ml at 0.59ml/s with 28.7psi vacuum @11:00:20.56 Cartridge 55 filtering 903/1000ml at 0.59ml/s with 26.6psi vacuum @11:01:20.57 Cartridge 55 filtering 939/1000ml at 0.59ml/s with 28.7psi vacuum @11:02:20.55 Cartridge 55 filtering 974/1000ml at 0.58ml/s with 23.4psi vacuum 5.1m deep (22.2psia) @11:03:05.72 Abs.stop @11:03:05.80 PhaseSeparator.stop @11:03:05.98 Intake.close @11:03:06.03 Exhaust.close @11:03:06.39 Toroid.in :hold @11:03:06.48 Toroid.seek :bypass @11:03:08.16 Toroid.in :default @11:03:08.26 Toroid.seek :clear @11:03:09.94 Sampled 1000.0ml @11:03:11.03 GoodNews.email "After filtering 1000.0ml",Subject: "Processing archiveNull_bac cartridge in slot 55" @11:03:11.61 Plunge.seek :reagent @11:03:12.45 Sample loop closed. Ready to Deliver Reagents @11:03:12.83 Carousel.in :default @11:03:13.00 Carousel.home.seek 55 @11:03:13.39 Plunge.seek :locked @11:03:14.43 Air.in :unlocking @11:03:14.59 Air.seek :clear @11:03:14.89 Lysis1.in :unlocking @11:03:15.27 Lysis1.seek :clear @11:03:16.90 Gate.power :analytic1,:OFF @11:03:16.92 Gate.power :skimmer,:OFF @11:03:16.98 Gate.power :motor,:OFF @11:03:17.00 Gate.power :core,:OFF @11:03:17.06 Gate.power :main,:OFF @11:03:17.08 Safely stopped and ready to power off @11:03:17.39 upsync @11:08:30.96 -> slot 38=>[:dry,:archiveNull_bac] {38 => { Type: :archiveNull_bac, State: :dry}} @11:08:33.38 -> slots {[38,19,11,5] => { Type: :archiveNull_bac, State: :dry}, 55 => { Type: :archiveNull_bac, State: :processed, filtered: 1000.0}} @11:08:50.35 -> BACsampler.goalvolume=300 @11:08:50.39 NoMethodError in n -- undefined method `goalvolume=' for BACsampler:Cartridge::Sampler @11:08:57.43 -> BACsampler.goalVolume=300 300 @11:09:10.22 -> deep;fire archiveNull_bac @11:09:10.46 Gate.power :sampler,:OFF,limit to 3500mA # @11:09:10.52 Gate.power :skimmer,:OFF,limit to 3500mA @11:09:10.59 Gate.power :main,:ON,limit to 5000mA @11:09:10.63 Gate.power :analytic1,:ON,limit to 4000mA @11:09:10.75 Powering core bus @11:09:13.53 Plunge.configure :default @11:09:13.65 SamplePump.configure :default @11:09:13.76 Toroid.configure :default @11:09:13.87 Carousel.configure :default @11:09:13.98 Abs.configure :default @11:09:14.09 PhaseSeparator.configure :full @11:09:14.19 Archive.configure :default @11:09:14.33 Air.configure :default @11:09:14.41 Lysis1.configure :default @11:09:14.76 Lysis2.configure :default @11:09:14.87 PinPort.configure :default @11:09:14.96 All elf controllers running firmware version 4.92 @11:09:14.99 Gate.power :motor,:ON,limit to 5000mA @11:09:17.99 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:09:18.02 AnalyticModule::Unknown in detached from n#3 -- SPR @11:09:18.33 Plunge.home.jog 0 @11:09:19.71 PinPort.home.jog 0 @11:09:19.75 Lysis1.home.jog 0 @11:09:19.86 Archive.home.jog 0 @11:09:19.89 Toroid.home.jog 0 @11:09:19.92 Lysis2.home.jog 0 @11:09:19.99 Air.home.jog 0 @11:09:20.03 Carousel.home.jog 0 @11:09:20.90 Plunge.in :homing @11:09:21.21 Plunge.jog 1615 @11:09:21.44 Lysis2.in :homing @11:09:21.54 Lysis1.in :homing @11:09:21.59 Archive.in :homing @11:09:21.64 Air.in :homing @11:09:21.69 PinPort.in :homing @11:09:21.74 Toroid.in :homing @11:09:22.06 Lysis2.jog 1408 @11:09:22.11 Lysis1.jog 1394 @11:09:22.17 Air.jog 1362 @11:09:22.24 Archive.jog 1395 @11:09:22.32 PinPort.jog -109 @11:09:22.41 Toroid.jog 610 @11:09:22.85 Plunge.setRawPosition! 50 @11:09:22.95 Plunge.seek :home @11:09:23.09 PinPort.setRawPosition! 0 @11:09:23.19 PinPort.seek :home @11:09:23.32 Plunge.in :default @11:09:23.45 PinPort.in :default @11:09:23.57 Lysis2.setRawPosition! 50 @11:09:23.64 Lysis2.seek :home @11:09:23.78 Lysis1.setRawPosition! 50 @11:09:23.81 Air.setRawPosition! 50 @11:09:23.98 Lysis1.seek :home @11:09:24.02 Air.seek :home @11:09:24.30 Archive.setRawPosition! 50 @11:09:24.33 Lysis2.in :default @11:09:24.50 Archive.seek :home @11:09:24.66 Air.in :default @11:09:24.68 Lysis1.in :default @11:09:24.88 Toroid.setRawPosition! 0 @11:09:24.91 Archive.in :default @11:09:25.06 Toroid.seek :home @11:09:25.21 Toroid.in :default @11:09:25.35 Toroid.seek :clear @11:09:27.05 Carousel.in :default @11:09:27.20 Carousel.home.seek 55 @11:09:27.49 Selecting Cartridge 38 @11:09:27.82 Carousel.seek 38 @11:09:31.65 Lysis1.in :fast @11:09:31.84 Lysis1.seek :locked @11:09:34.50 Plunge.seek :locked @11:09:34.70 Air.in :fast @11:09:34.79 Air.seek :locked @11:09:35.79 Carousel.reconfigure :locked @11:09:35.98 Powering cartridge bus @11:09:38.69 Gate.power :cartridge,:OFF @11:09:39.26 Mounted archive Cartridge @11:09:39.35 BACsampler with {source: :sampler} @11:09:39.37 GoodNews.email "BACsampler with {source: :sampler} filtering up to 300 ml",Subject: "Sampling archiveNull_bac cartridge in slot 38" @11:09:39.76 Preparing to filter cartridge 38 @11:09:40.09 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:09:44.74 upsync @11:09:49.23 Intake Head Pressure tare is 3% of full scale at 20C (drifted 2%) @11:09:49.24 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @11:09:49.33 PhaseSeparator.in :filling @11:09:49.41 PhaseSeparator.hold @11:09:49.52 Gate.power :sampler,:ON @11:09:57.85 Head pressure reached 0.98psi in 9 seconds @11:09:57.97 Priming sample loop w/20ml, bypass w/20ml @11:09:57.99 SamplePump.setPosition! 0ml @11:09:58.09 Bypass.open @11:09:59.56 Intake.open @11:10:00.37 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:10:03.00 Vacuum tare is 52% of full scale at 20C (drifted 1%) @11:10:03.28 Abs.in :default @11:10:03.36 Abs.hold @11:10:03.48 PhaseSeparator.in :full @11:10:03.55 PhaseSeparator.hold @11:10:03.81 Waiting for sampler to finish priming... @11:10:03.83 delayUntil "11:11:19.54" @11:11:19.60 SamplePump.in :start @11:11:19.71 SamplePump.seek 20ml,1:50 @11:11:20.30 SamplePump.configure :prime @11:11:44.51 Abs.stop @11:11:44.59 PhaseSeparator.stop @11:11:44.68 SamplePump.setPosition! 0ml @11:11:44.76 Exhaust.open @11:11:46.15 Bypass.close @11:11:47.69 Abs.in :default @11:11:47.78 Abs.hold @11:11:47.90 PhaseSeparator.hold @11:11:48.29 SamplePump.in :start @11:11:48.39 SamplePump.seek 20ml,1:50 @11:11:48.97 SamplePump.configure :prime @11:12:13.18 Abs.stop @11:12:13.28 PhaseSeparator.stop @11:12:13.39 Bypass.open @11:12:14.08 Intake.close @11:12:14.85 Toroid.in :hold @11:12:14.94 Toroid.seek :crack @11:12:16.51 Toroid.seek :cartridge @11:12:17.66 Toroid.in :default @11:12:17.76 Toroid.seek :clear @11:12:19.83 Pressurizing cartridge to 26psi above 15.2psia @11:12:19.85 SamplePump.setPosition! 0ml @11:12:19.95 Intake.open @11:12:20.63 Bypass.close @11:12:21.32 Exhaust.close @11:12:22.14 Abs.in :default @11:12:22.22 Abs.hold @11:12:22.36 PhaseSeparator.hold @11:12:22.63 SamplePump.in :charge @11:12:23.31 SamplePump.seek 2.3ml,42 seconds @11:12:36.73 Abs.stop @11:12:36.81 PhaseSeparator.stop @11:12:36.91 Intake.close @11:12:37.68 Pressurized cartridge with 0.806ml to 26.2psi @11:12:37.70 Isolated @39.7psia (24.6psi) @11:12:37.72 Verifying cartridge holds at least 0.65..0.85 of 39.7psia @11:12:37.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:12:42.42 Cartridge pressure fell 2% to 39.0psia @11:12:42.56 Sampling 300.0ml @11:12:42.59 SamplePump.setPosition! 0ml @11:12:42.70 Exhaust.open @11:12:43.38 Intake.open @11:12:44.30 Abs.in :default @11:12:44.39 Abs.hold @11:12:44.51 PhaseSeparator.hold @11:12:44.69 Sea 5.1m deep (22.3psia) @11:12:44.93 SamplePump.in :start @11:12:45.03 SamplePump.seek 300ml,25:30 @11:12:45.63 SamplePump.configure :sample @11:12:48.03 Cartridge 38 filtering 1/300ml at 0.55ml/s with 25.6psi vacuum @11:13:48.10 Cartridge 38 filtering 21/300ml at 0.32ml/s with 24.0psi vacuum @11:14:48.09 Cartridge 38 filtering 36/300ml at 0.26ml/s with 24.9psi vacuum @11:15:48.09 Cartridge 38 filtering 49/300ml at 0.21ml/s with 28.3psi vacuum @11:16:48.09 Cartridge 38 filtering 59/300ml at 0.17ml/s with 23.4psi vacuum @11:16:48.17 SamplePump.stop @11:16:48.29 Intake.close @11:16:48.41 Exhaust.close @11:16:48.69 Toroid.in :hold @11:16:48.80 Toroid.seek :bypass @11:16:50.49 Toroid.in :default @11:16:50.60 Toroid.seek :clear @11:16:52.28 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @11:16:52.31 Abs.stop @11:16:52.42 PhaseSeparator.stop @11:16:52.69 Sampled 59.2ml @11:16:53.14 GoodNews.email "After filtering 59.2ml",Subject: "Processing archiveNull_bac cartridge in slot 38" @11:16:54.36 Plunge.seek :reagent @11:16:55.25 Sample loop closed. Ready to Deliver Reagents @11:16:55.67 Carousel.in :default @11:16:55.83 Carousel.home.seek 38 @11:16:55.97 Gate.power :sampler,:OFF @11:16:56.34 Plunge.seek :locked @11:16:57.59 Air.in :unlocking @11:16:57.91 Air.seek :clear @11:16:58.14 Lysis1.in :unlocking @11:16:58.35 Lysis1.seek :clear @11:16:59.90 Gate.power :analytic1,:OFF @11:16:59.93 Gate.power :motor,:OFF @11:16:59.98 upsync @11:17:00.02 Gate.power :core,:OFF @11:17:00.08 Gate.power :main,:OFF @11:17:00.12 Safely stopped and ready to power off @11:24:25.97 -> Cartridge.unlock @11:24:26.06 BusOff in n -- core bus was not powered while reading Handoff Pin Port (PP)'s status @11:32:29.44 -> slot 32=>[:dry,:archiveNull_bac] {32 => { Type: :archiveNull_bac, State: :dry}} @11:32:38.54 -> slots {[32,19,11,5] => { Type: :archiveNull_bac, State: :dry}, 38 => { Type: :archiveNull_bac, State: :processed, filtered: 59.2}, 55 => { Type: :archiveNull_bac, State: :processed, filtered: 1000.0}} @11:33:02.66 -> deep;fire archiveNull_bac @11:33:02.87 Gate.power :sampler,:OFF,limit to 3500mA # @11:33:02.95 Gate.power :skimmer,:OFF,limit to 3500mA @11:33:03.01 Gate.power :main,:ON,limit to 5000mA @11:33:03.03 Gate.power :analytic1,:ON,limit to 4000mA @11:33:03.15 Powering core bus @11:33:05.96 Plunge.configure :default @11:33:06.07 SamplePump.configure :default @11:33:06.18 Toroid.configure :default @11:33:06.31 Carousel.configure :default @11:33:06.42 Abs.configure :default @11:33:06.53 PhaseSeparator.configure :full @11:33:06.64 Archive.configure :default @11:33:06.75 Air.configure :default @11:33:06.83 Lysis1.configure :default @11:33:07.18 Lysis2.configure :default @11:33:07.30 PinPort.configure :default @11:33:07.38 All elf controllers running firmware version 4.92 @11:33:07.41 Gate.power :motor,:ON,limit to 5000mA @11:33:10.41 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:33:10.44 AnalyticModule::Unknown in detached from n#3 -- SPR @11:33:10.76 Plunge.home.jog 0 @11:33:11.92 PinPort.home.jog 0 @11:33:12.05 Lysis2.home.jog 0 @11:33:12.10 Lysis1.home.jog 0 @11:33:12.16 Archive.home.jog 0 @11:33:12.20 Carousel.home.jog 0 @11:33:12.31 Air.home.jog 0 @11:33:12.37 Toroid.home.jog 0 @11:33:13.26 Plunge.in :homing @11:33:13.49 Plunge.jog 373 @11:33:13.72 Lysis2.in :homing @11:33:13.79 Lysis1.in :homing @11:33:13.84 Archive.in :homing @11:33:13.89 PinPort.in :homing @11:33:13.94 Air.in :homing @11:33:13.99 Toroid.in :homing @11:33:14.31 Lysis2.jog 1408 @11:33:14.37 Lysis1.jog 1379 @11:33:14.42 PinPort.jog -109 @11:33:14.50 Archive.jog 1387 @11:33:14.54 Air.jog 1360 @11:33:14.63 Toroid.jog 627 @11:33:15.09 PinPort.setRawPosition! 0 @11:33:15.12 Plunge.setRawPosition! 50 @11:33:15.28 PinPort.seek :home @11:33:15.31 Plunge.seek :home @11:33:15.56 PinPort.in :default @11:33:15.73 Plunge.in :default @11:33:15.84 Lysis2.setRawPosition! 50 @11:33:15.93 Lysis2.seek :home @11:33:16.09 Lysis1.setRawPosition! 50 @11:33:16.14 Air.setRawPosition! 50 @11:33:16.22 Archive.setRawPosition! 50 @11:33:16.37 Lysis1.seek :home @11:33:16.47 Air.seek :home @11:33:16.54 Archive.seek :home @11:33:16.84 Lysis2.in :default @11:33:16.98 Lysis1.in :default @11:33:17.04 Air.in :default @11:33:17.09 Archive.in :default @11:33:17.33 Toroid.setRawPosition! 0 @11:33:17.42 Toroid.seek :home @11:33:17.55 Toroid.in :default @11:33:17.70 Toroid.seek :clear @11:33:19.43 Carousel.in :default @11:33:19.59 Carousel.home.seek 39 @11:33:19.90 Selecting Cartridge 32 @11:33:20.22 Carousel.seek 32 @11:33:22.04 Lysis1.in :fast @11:33:22.13 Lysis1.seek :locked @11:33:24.88 Plunge.seek :locked @11:33:25.08 Air.in :fast @11:33:25.16 Air.seek :locked @11:33:26.16 Carousel.reconfigure :locked @11:33:26.38 Powering cartridge bus @11:33:29.06 Gate.power :cartridge,:OFF @11:33:29.63 Mounted archive Cartridge @11:33:29.71 BACsampler with {source: :sampler} @11:33:29.73 GoodNews.email "BACsampler with {source: :sampler} filtering up to 300 ml",Subject: "Sampling archiveNull_bac cartridge in slot 32" @11:33:30.13 Preparing to filter cartridge 32 @11:33:30.50 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:33:35.15 upsync @11:33:37.12 Intake Head Pressure tare is 3% of full scale at 19C (drifted 2%) @11:33:37.14 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @11:33:37.23 PhaseSeparator.in :filling @11:33:37.31 PhaseSeparator.hold @11:33:37.43 Gate.power :sampler,:ON @11:33:45.72 Head pressure reached 1.08psi in 9 seconds @11:33:45.84 Priming sample loop w/20ml, bypass w/20ml @11:33:45.87 SamplePump.setPosition! 0ml @11:33:45.96 Bypass.open @11:33:47.46 Intake.open @11:33:48.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:33:50.93 Vacuum tare is 52% of full scale at 19C (drifted 1%) @11:33:51.12 Abs.in :default @11:33:51.21 Abs.hold @11:33:51.82 PhaseSeparator.in :full @11:33:52.00 PhaseSeparator.hold @11:33:52.29 Waiting for sampler to finish priming... @11:33:52.30 delayUntil "11:35:07.45" @11:35:07.52 SamplePump.in :start @11:35:07.63 SamplePump.seek 20ml,1:50 @11:35:08.22 SamplePump.configure :prime @11:35:32.41 Abs.stop @11:35:32.50 PhaseSeparator.stop @11:35:32.59 SamplePump.setPosition! 0ml @11:35:32.67 Exhaust.open @11:35:34.07 Bypass.close @11:35:35.66 Abs.in :default @11:35:35.75 Abs.hold @11:35:35.88 PhaseSeparator.hold @11:35:36.32 SamplePump.in :start @11:35:36.43 SamplePump.seek 20ml,1:50 @11:35:37.01 SamplePump.configure :prime @11:36:01.21 Abs.stop @11:36:01.29 PhaseSeparator.stop @11:36:01.40 Bypass.open @11:36:02.06 Intake.close @11:36:02.87 Toroid.in :hold @11:36:02.95 Toroid.seek :crack @11:36:04.53 Toroid.seek :cartridge @11:36:05.67 Toroid.in :default @11:36:05.76 Toroid.seek :clear @11:36:07.84 Pressurizing cartridge to 26psi above 15.1psia @11:36:07.87 SamplePump.setPosition! 0ml @11:36:07.97 Intake.open @11:36:08.69 Bypass.close @11:36:09.36 Exhaust.close @11:36:10.17 Abs.in :default @11:36:10.28 Abs.hold @11:36:10.40 PhaseSeparator.hold @11:36:10.67 SamplePump.in :charge @11:36:10.78 SamplePump.seek 2.3ml,42 seconds @11:36:15.65 Abs.stop @11:36:15.74 PhaseSeparator.stop @11:36:15.84 Intake.close @11:36:16.65 Pressurized cartridge with 0.103ml to 26.1psi @11:36:16.66 Isolated @37.7psia (22.6psi) @11:36:16.70 Verifying cartridge holds at least 0.65..0.85 of 37.7psia @11:36:16.76 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:36:21.37 Cartridge pressure fell 2% to 37.0psia @11:36:21.51 Sampling 300.0ml @11:36:21.52 SamplePump.setPosition! 0ml @11:36:21.63 Exhaust.open @11:36:22.32 Intake.open @11:36:23.25 Abs.in :default @11:36:23.34 Abs.hold @11:36:23.45 PhaseSeparator.hold @11:36:23.63 Sea 12.8m deep (33.5psia) @11:36:23.87 SamplePump.in :start @11:36:23.97 SamplePump.seek 300ml,25:30 @11:36:24.55 SamplePump.configure :sample @11:36:26.96 Cartridge 32 filtering 0/300ml at 0.03ml/s with 22.8psi vacuum @11:37:27.01 Cartridge 32 filtering 0/300ml at 0.00ml/s with 23.6psi vacuum @11:37:27.07 SamplePump.stop @11:37:27.20 Intake.close @11:37:27.24 Exhaust.close @11:37:27.58 Toroid.in :hold @11:37:27.67 Toroid.seek :bypass @11:37:29.35 Toroid.in :default @11:37:29.45 Toroid.seek :clear @11:37:31.10 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @11:37:31.13 Abs.stop @11:37:31.22 PhaseSeparator.stop @11:37:31.49 Sampled 0.1ml @11:37:31.95 GoodNews.email "After filtering 0.1ml",Subject: "Processing archiveNull_bac cartridge in slot 32" @11:37:32.62 Plunge.seek :reagent @11:37:33.48 Sample loop closed. Ready to Deliver Reagents @11:37:34.44 Carousel.in :default @11:37:34.63 Carousel.home.seek 32 @11:37:34.74 Gate.power :sampler,:OFF @11:37:35.06 Plunge.seek :locked @11:37:36.25 Air.in :unlocking @11:37:36.49 Air.seek :clear @11:37:36.64 Lysis1.in :unlocking @11:37:36.78 Lysis1.seek :clear @11:37:37.78 upsync @11:37:38.36 Gate.power :analytic1,:OFF @11:37:38.38 Gate.power :motor,:OFF @11:37:38.42 Gate.power :core,:OFF @11:37:38.46 Gate.power :main,:OFF @11:37:38.51 Safely stopped and ready to power off @11:40:24.04 -> slot 32=>:dry {32 => { Type: :archiveNull_bac, State: :dry, filtered: 0.1}} @11:40:40.87 -> slot 32=>[:dry,:archiveNull_bac] {32 => { Type: :archiveNull_bac, State: :dry}} @11:40:44.02 -> deep;fire archiveNull_bac # @11:40:44.31 Gate.power :sampler,:OFF,limit to 3500mA @11:40:44.36 Gate.power :skimmer,:OFF,limit to 3500mA @11:40:44.40 Gate.power :main,:ON,limit to 5000mA @11:40:44.45 Gate.power :analytic1,:ON,limit to 4000mA @11:40:44.58 Powering core bus @11:40:47.37 Plunge.configure :default @11:40:47.49 SamplePump.configure :default @11:40:47.60 Toroid.configure :default @11:40:47.71 Carousel.configure :default @11:40:47.81 Abs.configure :default @11:40:47.92 PhaseSeparator.configure :full @11:40:48.01 Archive.configure :default @11:40:48.12 Air.configure :default @11:40:48.20 Lysis1.configure :default @11:40:48.58 Lysis2.configure :default @11:40:48.69 PinPort.configure :default @11:40:48.77 All elf controllers running firmware version 4.92 @11:40:48.80 Gate.power :motor,:ON,limit to 5000mA @11:40:51.82 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:40:51.85 AnalyticModule::Unknown in detached from n#3 -- SPR @11:40:52.21 Toroid.home.jog 0 @11:40:52.41 Plunge.home.jog 0 @11:40:53.04 Carousel.home.jog 0 @11:40:53.15 Lysis2.home.jog 0 @11:40:53.22 Air.home.jog 0 @11:40:53.36 Lysis1.home.jog 0 @11:40:53.38 Archive.home.jog 0 @11:40:53.43 PinPort.home.jog 0 @11:40:54.22 Plunge.in :homing @11:40:54.32 Toroid.in :homing @11:40:54.49 Plunge.jog 1610 @11:40:54.55 Toroid.jog 607 @11:40:55.32 PinPort.in :homing @11:40:55.47 Lysis2.in :homing @11:40:55.52 Air.in :homing @11:40:55.57 Archive.in :homing @11:40:55.61 Lysis1.in :homing @11:40:55.87 PinPort.jog -109 @11:40:55.93 Lysis2.jog 1408 @11:40:56.01 Air.jog 1367 @11:40:56.06 Archive.jog 1395 @11:40:56.15 Lysis1.jog 1397 @11:40:56.57 PinPort.setRawPosition! 0 @11:40:56.60 Plunge.setRawPosition! 50 @11:40:56.77 PinPort.seek :home @11:40:56.80 Plunge.seek :home @11:40:57.08 PinPort.in :default @11:40:57.14 Toroid.setRawPosition! 0 @11:40:57.26 Toroid.seek :home @11:40:57.40 Plunge.in :default @11:40:57.44 Toroid.in :default @11:40:57.66 Lysis1.setRawPosition! 50 @11:40:57.70 Air.setRawPosition! 50 @11:40:57.75 Lysis2.setRawPosition! 50 @11:40:57.79 Archive.setRawPosition! 50 @11:40:58.01 Lysis1.seek :home @11:40:58.09 Air.seek :home @11:40:58.15 Lysis2.seek :home @11:40:58.26 Archive.seek :home @11:40:58.60 Lysis1.in :default @11:40:58.77 Lysis2.in :default @11:40:58.83 Air.in :default @11:40:58.90 Archive.in :default @11:40:59.34 Toroid.seek :clear @11:41:01.08 Carousel.in :default @11:41:01.23 Carousel.home.seek 32 @11:41:01.53 Selecting Cartridge 32 @11:41:01.85 Carousel.seek 32 @11:41:02.12 Lysis1.in :fast @11:41:02.21 Lysis1.seek :locked @11:41:04.90 Plunge.seek :locked @11:41:05.10 Air.in :fast @11:41:05.18 Air.seek :locked @11:41:06.18 Carousel.reconfigure :locked @11:41:06.39 Powering cartridge bus @11:41:09.08 Gate.power :cartridge,:OFF @11:41:09.65 Mounted archive Cartridge @11:41:09.74 BACsampler with {source: :sampler} @11:41:09.76 GoodNews.email "BACsampler with {source: :sampler} filtering up to 300 ml",Subject: "Sampling archiveNull_bac cartridge in slot 32" @11:41:10.13 Preparing to filter cartridge 32 @11:41:11.04 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:41:15.54 upsync @11:41:21.67 Intake Head Pressure tare is 3% of full scale at 20C (drifted 2%) @11:41:21.69 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @11:41:21.76 PhaseSeparator.in :filling @11:41:21.84 PhaseSeparator.hold @11:41:21.95 Gate.power :sampler,:ON @11:41:30.20 Head pressure reached 0.96psi in 9 seconds @11:41:30.35 Priming sample loop w/20ml, bypass w/20ml @11:41:30.37 SamplePump.setPosition! 0ml @11:41:30.46 Bypass.open @11:41:31.93 Intake.open @11:41:32.66 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:41:35.29 Vacuum tare is 52% of full scale at 20C (drifted 1%) @11:41:35.49 Abs.in :default @11:41:35.58 Abs.hold @11:41:35.69 PhaseSeparator.in :full @11:41:35.76 PhaseSeparator.hold @11:41:36.02 Waiting for sampler to finish priming... @11:41:36.04 delayUntil "11:42:51.97" @11:42:52.03 SamplePump.in :start @11:42:52.14 SamplePump.seek 20ml,1:50 @11:42:52.73 SamplePump.configure :prime @11:43:17.42 Abs.stop @11:43:17.60 PhaseSeparator.stop @11:43:17.69 SamplePump.setPosition! 0ml @11:43:17.76 Exhaust.open @11:43:19.15 Bypass.close @11:43:20.71 Abs.in :default @11:43:20.81 Abs.hold @11:43:20.92 PhaseSeparator.hold @11:43:21.27 SamplePump.in :start @11:43:21.37 SamplePump.seek 20ml,1:50 @11:43:21.96 SamplePump.configure :prime @11:43:46.15 Abs.stop @11:43:46.26 PhaseSeparator.stop @11:43:46.39 Bypass.open @11:43:47.10 Intake.close @11:43:47.88 Toroid.in :hold @11:43:47.96 Toroid.seek :crack @11:43:49.53 Toroid.seek :cartridge @11:43:50.67 Toroid.in :default @11:43:50.76 Toroid.seek :clear @11:43:52.83 Pressurizing cartridge to 26psi above 15.1psia @11:43:52.85 SamplePump.setPosition! 0ml @11:43:52.94 Intake.open @11:43:53.65 Bypass.close @11:43:54.34 Exhaust.close @11:43:55.14 Abs.in :default @11:43:55.22 Abs.hold @11:43:55.34 PhaseSeparator.hold @11:43:55.61 SamplePump.in :charge @11:43:55.71 SamplePump.seek 2.3ml,42 seconds @11:44:08.98 Abs.stop @11:44:09.08 PhaseSeparator.stop @11:44:09.18 Intake.close @11:44:09.99 Pressurized cartridge with 0.788ml to 26.9psi @11:44:10.00 Isolated @39.4psia (24.3psi) @11:44:10.05 Verifying cartridge holds at least 0.65..0.85 of 39.4psia @11:44:10.11 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:44:15.45 Sampling 300.0ml @11:44:15.48 SamplePump.setPosition! 0ml @11:44:15.59 Exhaust.open @11:44:16.29 Intake.open @11:44:17.23 Abs.in :default @11:44:17.32 Abs.hold @11:44:17.43 PhaseSeparator.hold @11:44:17.84 SamplePump.in :start @11:44:17.94 SamplePump.seek 300ml,25:30 @11:44:18.52 SamplePump.configure :sample @11:44:20.93 Cartridge 32 filtering 1/300ml at 0.25ml/s with 23.1psi vacuum @11:45:20.98 Cartridge 32 filtering 8/300ml at 0.11ml/s with 22.8psi vacuum @11:45:21.03 SamplePump.stop @11:45:21.18 Intake.close @11:45:21.29 Exhaust.close @11:45:21.56 Toroid.in :hold @11:45:21.65 Toroid.seek :bypass @11:45:23.32 Toroid.in :default @11:45:23.42 Toroid.seek :clear @11:45:25.08 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @11:45:25.10 Abs.stop @11:45:25.22 PhaseSeparator.stop @11:45:25.49 Sampled 7.8ml @11:45:25.95 GoodNews.email "After filtering 7.8ml",Subject: "Processing archiveNull_bac cartridge in slot 32" @11:45:26.62 Plunge.seek :reagent @11:45:27.50 Sample loop closed. Ready to Deliver Reagents @11:45:27.85 Carousel.in :default @11:45:28.03 Carousel.home.seek 32 @11:45:28.47 Plunge.seek :locked @11:45:28.65 Gate.power :sampler,:OFF @11:45:30.47 Air.in :unlocking @11:45:30.79 Air.seek :clear @11:45:30.91 Lysis1.in :unlocking @11:45:31.11 Lysis1.seek :clear @11:45:32.43 upsync @11:45:32.67 Gate.power :analytic1,:OFF @11:45:32.72 Gate.power :motor,:OFF @11:45:32.75 Gate.power :core,:OFF @11:45:32.79 Gate.power :main,:OFF @11:45:32.83 Safely stopped and ready to power off @11:52:48.67 -> Cartridge.unlock @11:52:48.75 BusOff in n -- core bus was not powered while reading Handoff Pin Port (PP)'s status @11:54:51.74 -> ESP.ready! @11:54:51.81 Gate.power :sampler,:OFF,limit to 3500mA @11:54:51.83 Gate.power :skimmer,:OFF,limit to 3500mA @11:54:51.90 Gate.power :main,:ON,limit to 5000mA @11:54:51.93 Gate.power :analytic1,:ON,limit to 4000mA @11:54:52.05 Powering core bus @11:54:54.83 Plunge.configure :default @11:54:54.96 SamplePump.configure :default @11:54:55.07 Toroid.configure :default @11:54:55.17 Carousel.configure :default @11:54:55.28 Abs.configure :default @11:54:55.40 PhaseSeparator.configure :full @11:54:55.52 Archive.configure :default @11:54:55.63 Air.configure :default @11:54:55.72 Lysis1.configure :default @11:54:56.07 Lysis2.configure :default @11:54:56.18 PinPort.configure :default @11:54:56.31 All elf controllers running firmware version 4.92 @11:54:56.35 Gate.power :motor,:ON,limit to 5000mA @11:54:59.31 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:54:59.34 AnalyticModule::Unknown in n -- SPR @11:54:59.60 Plunge.home.jog 0 @11:55:00.35 Lysis1.home.jog 0 @11:55:00.43 Air.home.jog 0 @11:55:00.47 Carousel.home.jog 0 @11:55:00.52 Toroid.home.jog 0 @11:55:00.58 Lysis2.home.jog 0 @11:55:00.62 Archive.home.jog 0 @11:55:00.70 PinPort.home.jog 0 @11:55:01.54 Plunge.in :homing @11:55:01.97 Plunge.jog 1614 @11:55:02.06 Lysis2.in :homing @11:55:02.12 Air.in :homing @11:55:02.17 Toroid.in :homing @11:55:02.21 PinPort.in :homing @11:55:02.29 Archive.in :homing @11:55:02.34 Lysis1.in :homing @11:55:02.74 Lysis2.jog 1408 @11:55:02.77 PinPort.jog -110 @11:55:02.83 Toroid.jog 620 @11:55:02.88 Air.jog 1366 @11:55:02.94 Lysis1.jog 1396 @11:55:02.99 Archive.jog 1394 @11:55:03.47 PinPort.setRawPosition! 0 @11:55:04.13 PinPort.seek :home @11:55:04.38 Lysis2.setRawPosition! 50 @11:55:04.44 Air.setRawPosition! 50 @11:55:04.48 Plunge.setRawPosition! 50 @11:55:04.54 PinPort.in :default @11:55:04.72 Air.seek :home @11:55:04.80 Lysis2.seek :home @11:55:04.86 Plunge.seek :home @11:55:05.17 Lysis1.setRawPosition! 50 @11:55:05.21 Archive.setRawPosition! 50 @11:55:05.37 Lysis1.seek :home @11:55:05.40 Archive.seek :home @11:55:05.65 Air.in :default @11:55:05.70 Toroid.setRawPosition! 0 @11:55:05.76 Lysis2.in :default @11:55:05.81 Plunge.in :default @11:55:05.99 Toroid.seek :home @11:55:06.21 Lysis1.in :default @11:55:06.26 Archive.in :default @11:55:06.36 Toroid.in :default @11:55:06.80 Toroid.seek :clear @11:55:08.51 Carousel.in :default @11:55:08.66 Carousel.home.seek 32 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 32 Abs = Absolute Pressure (Leak) Sensor @24psia PhaseSeparator = Intake Head Pressure @-0.01psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:55:15.54 -> BACsampler.source=skimmer :skimmer @11:55:19.37 -> BACsampler.prime @11:55:19.49 Priming sample loop w/20ml, bypass w/20ml @11:55:19.51 SamplePump.setPosition! 0ml @11:55:19.60 Bypass.open @11:55:21.11 Intake.open @11:55:21.92 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:55:24.54 Vacuum tare is 52% of full scale at 20C (drifted 1%) @11:55:24.72 Abs.in :default @11:55:24.80 Abs.hold @11:55:24.91 PhaseSeparator.hold @11:55:25.17 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:55:25.33 SamplePump.stop @11:55:25.48 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.04psi @11:55:25.51 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @11:55:25.59 PhaseSeparator.in :filling @11:55:25.67 PhaseSeparator.hold @11:55:25.78 Gate.power :skimmer,:ON @11:55:35.62 Head pressure reached 0.80psi in 11 seconds @11:55:35.64 Abs.hold @11:55:36.22 PhaseSeparator.in :full @11:55:36.45 PhaseSeparator.hold @11:55:36.71 Waiting for skimmer to finish priming... @11:55:36.72 delayUntil "11:56:55.80" @11:56:55.88 SamplePump.in :start @11:56:55.97 SamplePump.seek 20ml,1:50 @11:56:56.56 SamplePump.configure :prime @11:57:20.77 Abs.stop @11:57:20.84 PhaseSeparator.stop @11:57:20.93 SamplePump.setPosition! 0ml @11:57:21.00 Exhaust.open @11:57:22.40 Bypass.close @11:57:23.91 Abs.in :default @11:57:24.00 Abs.hold @11:57:24.11 PhaseSeparator.hold @11:57:24.52 SamplePump.in :start @11:57:24.61 SamplePump.seek 20ml,1:50 @11:57:25.21 SamplePump.configure :prime @11:57:49.41 Abs.stop @11:57:49.49 PhaseSeparator.stop BACsampler @12:11:04.47 -> Power.sampler :off @12:11:04.50 Gate.power :sampler,:OFF false @12:14:03.45 -> Power.skimmer :off;BACsampler.soure=sampler;BACsampler.prime;delay 10.minutes;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime;delay 10.minutes @12:14:03.48 Gate.power :skimmer,:OFF @12:14:03.54 NoMethodError in n -- undefined method `soure=' for BACsampler:Cartridge::Sampler @12:14:20.13 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;delay 10.minutes;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime;delay 10.minutes @12:14:20.15 Gate.power :skimmer,:OFF @12:14:20.32 Priming sample loop w/20ml, bypass w/20ml @12:14:20.34 SamplePump.setPosition! 0ml @12:14:20.43 Exhaust.close @12:14:21.08 Bypass.open @12:14:22.70 Abs.in :default @12:14:22.78 Abs.hold @12:14:22.88 PhaseSeparator.hold @12:14:23.15 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:14:23.30 SamplePump.stop @12:14:23.46 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.02psi @12:14:23.49 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @12:14:23.57 PhaseSeparator.in :filling @12:14:23.66 PhaseSeparator.hold @12:14:23.78 Gate.power :sampler,:ON @12:14:32.03 Head pressure reached 0.87psi in 9 seconds @12:14:32.05 Abs.hold @12:14:32.19 PhaseSeparator.in :full @12:14:32.31 PhaseSeparator.hold @12:14:32.58 Waiting for sampler to finish priming... @12:14:32.59 delayUntil "12:15:53.80" @12:14:37.09 Intake.close @12:14:37.23 Bypass.close @12:14:38.00 Abs.stop @12:14:38.08 PhaseSeparator.stop @12:14:38.17 UserAbort in n -- User Abort!! @12:14:43.19 -> BACsampler.primeVolume 20 @12:14:47.76 -> BACsampler.primeVolume=150 150 @12:14:55.42 -> BACsampler.bypassVolume=50 50 @12:14:59.16 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;delay 10.minutes;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime;delay 10.minutes @12:14:59.18 Gate.power :skimmer,:OFF @12:14:59.32 Priming sample loop w/150ml, bypass w/50ml @12:14:59.34 SamplePump.setPosition! 0ml @12:14:59.44 Bypass.open @12:15:01.00 Intake.open @12:15:02.53 Abs.in :default @12:15:02.62 Abs.hold @12:15:02.73 PhaseSeparator.hold @12:15:02.98 Waiting for sampler to finish priming... @12:15:03.00 delayUntil "12:15:53.80" @12:15:53.87 SamplePump.in :start @12:15:53.95 SamplePump.seek 50ml,3:50 @12:15:54.56 SamplePump.configure :prime @12:16:55.37 Abs.stop @12:16:55.45 PhaseSeparator.stop @12:16:55.54 SamplePump.setPosition! 0ml @12:16:55.63 Exhaust.open @12:16:57.01 Bypass.close @12:16:58.58 Abs.in :default @12:16:58.66 Abs.hold @12:16:58.77 PhaseSeparator.hold @12:16:59.12 SamplePump.in :start @12:16:59.21 SamplePump.seek 150ml,10:30 @12:16:59.81 SamplePump.configure :prime @12:20:02.75 Abs.stop @12:20:02.83 PhaseSeparator.stop @12:20:02.91 delay 10:00 @12:30:02.94 Gate.power :sampler,:OFF @12:30:03.06 Priming sample loop w/150ml, bypass w/50ml @12:30:03.08 SamplePump.setPosition! 0ml @12:30:03.17 Exhaust.close @12:30:03.82 Bypass.open @12:30:05.48 Abs.in :default @12:30:05.56 Abs.hold @12:30:05.67 PhaseSeparator.hold @12:30:05.93 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:30:06.09 SamplePump.stop @12:30:06.24 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to -0.04psi @12:30:06.29 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @12:30:06.36 PhaseSeparator.in :filling @12:30:06.44 PhaseSeparator.hold @12:30:06.55 Gate.power :skimmer,:ON @12:30:16.74 Head pressure reached 0.77psi in 11 seconds @12:30:16.76 Abs.hold @12:30:16.90 PhaseSeparator.in :full @12:30:16.97 PhaseSeparator.hold @12:30:17.23 Waiting for skimmer to finish priming... @12:30:17.24 delayUntil "12:31:36.57" @12:31:36.65 SamplePump.in :start @12:31:37.34 SamplePump.seek 50ml,3:50 @12:31:37.44 SamplePump.configure :prime @12:32:38.57 Abs.stop @12:32:38.65 PhaseSeparator.stop @12:32:38.73 SamplePump.setPosition! 0ml @12:32:38.81 Exhaust.open @12:32:40.16 Bypass.close @12:32:41.69 Abs.in :default @12:32:41.77 Abs.hold @12:32:41.88 PhaseSeparator.hold @12:32:42.24 SamplePump.in :start @12:32:42.35 SamplePump.seek 150ml,10:30 @12:32:42.92 SamplePump.configure :prime @12:35:45.80 Abs.stop @12:35:45.88 PhaseSeparator.stop @12:35:45.96 delay 10:00 @12:54:32.78 -> Power.sampler :off @12:54:32.80 Gate.power :sampler,:OFF false @12:54:36.59 -> Power.skimmer :off @12:54:36.61 Gate.power :skimmer,:OFF false @13:01:53.80 -> Power.skimmer :on @13:01:53.83 Gate.power :skimmer,:ON true @13:02:07.96 -> BACsampler.prime @13:02:08.08 Priming sample loop w/150ml, bypass w/50ml @13:02:08.10 SamplePump.setPosition! 0ml @13:02:08.20 Exhaust.close @13:02:08.88 Bypass.open @13:02:10.51 Abs.in :default @13:02:10.59 Abs.hold @13:02:10.69 PhaseSeparator.hold @13:02:10.95 Waiting for skimmer to finish priming... @13:02:10.97 delayUntil "13:03:23.86" @13:02:53.33 Intake.close @13:02:53.40 Bypass.close @13:02:54.29 Abs.stop @13:02:54.37 PhaseSeparator.stop @13:02:54.45 UserAbort in n -- User Abort!! @13:03:00.66 -> BACsampler.source=skimmer :skimmer @13:03:05.79 -> BACsampler.prime @13:03:05.92 Priming sample loop w/150ml, bypass w/50ml @13:03:05.94 SamplePump.setPosition! 0ml @13:03:06.04 Bypass.open @13:03:07.62 Intake.open @13:03:09.15 Abs.in :default @13:03:09.23 Abs.hold @13:03:09.34 PhaseSeparator.hold @13:03:09.59 Waiting for skimmer to finish priming... @13:03:09.60 delayUntil "13:03:23.86" @13:03:23.94 SamplePump.in :start @13:03:24.04 SamplePump.seek 50ml,3:50 @13:03:24.63 SamplePump.configure :prime @13:04:25.44 Abs.stop @13:04:25.52 PhaseSeparator.stop @13:04:26.20 SamplePump.setPosition! 0ml @13:04:26.32 Exhaust.open @13:04:27.67 Bypass.close @13:04:29.24 Abs.in :default @13:04:29.33 Abs.hold @13:04:29.43 PhaseSeparator.hold @13:04:29.79 SamplePump.in :start @13:04:29.88 SamplePump.seek 150ml,10:30 @13:04:30.48 SamplePump.configure :prime @13:07:33.33 Abs.stop @13:07:33.40 PhaseSeparator.stop BACsampler @13:08:25.72 -> Power.skimmer :off @13:08:25.75 Gate.power :skimmer,:OFF false @13:19:29.12 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime @13:19:29.14 Gate.power :skimmer,:OFF @13:19:29.27 Priming sample loop w/150ml, bypass w/50ml @13:19:29.29 SamplePump.setPosition! 0ml @13:19:29.38 Exhaust.close @13:19:30.01 Bypass.open @13:19:31.65 Abs.in :default @13:19:31.73 Abs.hold @13:19:31.84 PhaseSeparator.hold @13:19:32.10 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @13:19:32.29 SamplePump.stop @13:19:32.44 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to -0.01psi @13:19:32.49 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:19:32.55 PhaseSeparator.in :filling @13:19:32.63 PhaseSeparator.hold @13:19:32.75 Gate.power :sampler,:ON @13:19:41.11 Head pressure reached 1.02psi in 9 seconds @13:19:41.13 Abs.hold @13:19:41.25 PhaseSeparator.in :full @13:19:41.33 PhaseSeparator.hold @13:19:41.60 Waiting for sampler to finish priming... @13:19:41.61 delayUntil "13:21:02.78" @13:21:02.85 SamplePump.in :start @13:21:02.94 SamplePump.seek 50ml,3:50 @13:21:03.54 SamplePump.configure :prime @13:22:04.38 Abs.stop @13:22:04.45 PhaseSeparator.stop @13:22:04.53 SamplePump.setPosition! 0ml @13:22:04.62 Exhaust.open @13:22:05.96 Bypass.close @13:22:07.48 Abs.in :default @13:22:07.56 Abs.hold @13:22:07.67 PhaseSeparator.hold @13:22:08.01 SamplePump.in :start @13:22:08.11 SamplePump.seek 150ml,10:30 @13:22:08.83 SamplePump.configure :prime @13:25:12.14 Abs.stop @13:25:12.22 PhaseSeparator.stop @13:25:12.34 Gate.power :sampler,:OFF @13:25:12.46 Priming sample loop w/150ml, bypass w/50ml @13:25:12.49 SamplePump.setPosition! 0ml @13:25:12.58 Exhaust.close @13:25:13.23 Bypass.open @13:25:14.91 Abs.in :default @13:25:15.00 Abs.hold @13:25:15.11 PhaseSeparator.hold @13:25:15.40 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @13:25:15.54 SamplePump.stop @13:25:15.70 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.07psi @13:25:15.73 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:25:15.80 PhaseSeparator.in :filling @13:25:15.89 PhaseSeparator.hold @13:25:16.01 Gate.power :skimmer,:ON @13:25:31.06 Gate.power :skimmer,:OFF @13:25:31.44 Gate.power :skimmer,:ON @13:25:35.19 Head pressure reached 0.80psi in 20 seconds @13:25:35.21 Abs.hold @13:25:35.34 PhaseSeparator.in :full @13:25:35.43 PhaseSeparator.hold @13:25:35.69 Waiting for skimmer to finish priming... @13:25:35.71 delayUntil "13:27:01.46" @13:27:01.52 SamplePump.in :start @13:27:01.62 SamplePump.seek 50ml,3:50 @13:27:02.22 SamplePump.configure :prime @13:28:03.03 Abs.stop @13:28:03.12 PhaseSeparator.stop @13:28:03.20 SamplePump.setPosition! 0ml @13:28:03.29 Exhaust.open @13:28:04.67 Bypass.close @13:28:06.22 Abs.in :default @13:28:06.34 Abs.hold @13:28:06.45 PhaseSeparator.hold @13:28:06.79 SamplePump.in :start @13:28:06.89 SamplePump.seek 150ml,10:30 @13:28:07.49 SamplePump.configure :prime @13:31:10.40 Abs.stop @13:31:10.49 PhaseSeparator.stop BACsampler @13:31:56.38 -> Power.skimmer :off @13:31:56.40 Gate.power :skimmer,:OFF false @13:32:49.28 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime @13:32:49.30 Gate.power :skimmer,:OFF @13:32:49.43 Priming sample loop w/150ml, bypass w/50ml @13:32:49.45 SamplePump.setPosition! 0ml @13:32:49.54 Exhaust.close @13:32:50.22 Bypass.open @13:32:51.93 Abs.in :default @13:32:52.00 Abs.hold @13:32:52.10 PhaseSeparator.hold @13:32:53.00 SamplePump.stop @13:32:53.15 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.01psi @13:32:53.17 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:32:53.26 PhaseSeparator.in :filling @13:32:53.35 PhaseSeparator.hold @13:32:53.46 Gate.power :sampler,:ON @13:33:02.13 Head pressure reached 0.92psi in 9 seconds @13:33:02.15 Abs.hold @13:33:02.34 PhaseSeparator.in :full @13:33:02.42 PhaseSeparator.hold @13:33:02.68 Waiting for sampler to finish priming... @13:33:02.70 delayUntil "13:34:23.48" @13:34:21.10 SamplePump.stop @13:34:21.24 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.42psi @13:34:21.27 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:34:21.35 PhaseSeparator.in :filling @13:34:21.43 PhaseSeparator.hold @13:34:21.55 Gate.power :sampler,:OFF @13:34:21.95 Gate.power :sampler,:ON @13:34:36.98 Gate.power :sampler,:OFF @13:34:37.36 Gate.power :sampler,:ON @13:34:52.40 Gate.power :sampler,:OFF @13:34:52.77 Gate.power :sampler,:ON @13:35:02.51 Head pressure reached 0.80psi in 42 seconds @13:35:02.53 Abs.hold @13:35:02.66 PhaseSeparator.in :full @13:35:02.74 PhaseSeparator.hold @13:35:02.99 Waiting for sampler to finish priming... @13:35:03.00 delayUntil "13:36:22.81" @13:35:40.59 SamplePump.stop @13:35:40.74 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.29psi @13:35:40.79 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:35:40.85 PhaseSeparator.in :filling @13:35:40.93 PhaseSeparator.hold @13:35:41.05 Gate.power :sampler,:OFF @13:35:41.43 Gate.power :sampler,:ON @13:35:56.49 Gate.power :sampler,:OFF @13:35:56.87 Gate.power :sampler,:ON @13:36:11.90 Gate.power :sampler,:OFF @13:36:12.28 Gate.power :sampler,:ON @13:36:27.32 Gate.power :sampler,:OFF @13:36:27.69 Gate.power :sampler,:ON @13:36:36.69 PhaseSeparator.stop @13:36:36.81 Abs.stop @13:36:36.91 UserAbort in n -- User Abort!! @13:36:42.79 -> Cmd.stop @13:36:42.91 Intake.close @13:36:42.96 Bypass.close @13:36:43.85 Carousel.in :default @13:36:44.00 Carousel.home.seek 32 @13:36:44.40 Lysis1.in :fast @13:36:44.49 Lysis1.seek :locked @13:36:47.05 Plunge.seek :locked @13:36:48.46 Lysis1.in :unlocking @13:36:48.63 Lysis1.seek :clear @13:36:50.16 Gate.power :analytic1,:OFF @13:36:50.20 Gate.power :sampler,:OFF @13:36:50.26 Gate.power :motor,:OFF @13:36:50.30 Gate.power :core,:OFF @13:36:50.33 Gate.power :main,:OFF @13:36:50.37 Safely stopped and ready to power off :STOPPED @13:42:17.39 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime @13:42:17.42 Gate.power :skimmer,:OFF @13:42:17.51 BusOff in n -- core bus was not powered while reading Storage Carousel (SC)'s status @13:42:26.22 -> ESP.ready! @13:42:26.34 Gate.power :sampler,:OFF,limit to 3500mA @13:42:26.36 Gate.power :skimmer,:OFF,limit to 3500mA @13:42:26.40 Gate.power :main,:ON,limit to 5000mA @13:42:26.44 Gate.power :analytic1,:ON,limit to 4000mA @13:42:26.55 Powering core bus @13:42:29.25 Plunge.configure :default @13:42:29.36 SamplePump.configure :default @13:42:29.47 Toroid.configure :default @13:42:29.58 Carousel.configure :default @13:42:29.69 Abs.configure :default @13:42:29.79 PhaseSeparator.configure :filling @13:42:29.90 Archive.configure :default @13:42:30.01 Air.configure :default @13:42:30.10 Lysis1.configure :default @13:42:30.18 Lysis2.configure :default @13:42:30.58 PinPort.configure :default @13:42:30.66 All elf controllers running firmware version 4.92 @13:42:30.69 Gate.power :motor,:ON,limit to 5000mA @13:42:33.84 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:42:33.87 AnalyticModule::Unknown in n -- SPR @13:42:34.28 Plunge.home.jog 0 @13:42:34.94 PinPort.home.jog 0 @13:42:35.00 Lysis1.home.jog 0 @13:42:35.06 Archive.home.jog 0 @13:42:35.09 Toroid.home.jog 0 @13:42:35.13 Lysis2.home.jog 0 @13:42:35.18 Air.home.jog 0 @13:42:35.25 Carousel.home.jog 0 @13:42:36.02 Plunge.in :homing @13:42:36.35 Plunge.jog 1612 @13:42:36.66 Lysis2.in :homing @13:42:36.72 Lysis1.in :homing @13:42:36.77 Archive.in :homing @13:42:36.81 Toroid.in :homing @13:42:36.88 Air.in :homing @13:42:36.97 PinPort.in :homing @13:42:37.10 Lysis2.jog 1407 @13:42:37.24 Lysis1.jog 1395 @13:42:37.39 Archive.jog 1395 @13:42:37.45 Air.jog 1362 @13:42:37.50 Toroid.jog 619 @13:42:37.55 PinPort.jog -108 @13:42:37.96 Plunge.setRawPosition! 50 @13:42:38.05 Plunge.seek :home @13:42:38.18 PinPort.setRawPosition! 0 @13:42:38.28 PinPort.seek :home @13:42:38.43 Plunge.in :default @13:42:38.51 PinPort.in :default @13:42:38.66 Lysis2.setRawPosition! 50 @13:42:38.73 Lysis2.seek :home @13:42:38.85 Lysis1.setRawPosition! 50 @13:42:38.95 Lysis1.seek :home @13:42:39.12 Lysis2.in :default @13:42:39.14 Air.setRawPosition! 50 @13:42:39.17 Archive.setRawPosition! 50 @13:42:39.35 Air.seek :home @13:42:39.41 Archive.seek :home @13:42:39.62 Lysis1.in :default @13:42:40.35 Air.in :default @13:42:40.57 Toroid.setRawPosition! 0 @13:42:40.61 Archive.in :default @13:42:40.74 Toroid.seek :home @13:42:40.90 Toroid.in :default @13:42:41.06 Toroid.seek :clear @13:42:42.75 Carousel.in :default @13:42:42.90 Carousel.home.seek 32 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 32 Abs = Absolute Pressure (Leak) Sensor @14psia PhaseSeparator = Intake Head Pressure @-0.01psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:42:45.77 -> Power.skimmer :off;BACsampler.source=sampler;BACsampler.prime;Power.sampler :off;BACsampler.source=skimmer;BACsampler.prime @13:42:45.80 Gate.power :skimmer,:OFF @13:42:45.93 Priming sample loop w/150ml, bypass w/50ml @13:42:45.95 SamplePump.setPosition! 0ml @13:42:46.05 Bypass.open @13:42:47.60 Intake.open @13:42:49.17 Abs.in :default @13:42:49.25 Abs.hold @13:42:49.36 PhaseSeparator.in :full @13:42:49.43 PhaseSeparator.hold @13:42:49.69 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @13:42:49.84 SamplePump.stop @13:42:50.00 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.03psi @13:42:50.03 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:42:50.10 PhaseSeparator.in :filling @13:42:50.19 PhaseSeparator.hold @13:42:50.34 Gate.power :sampler,:ON @13:42:59.18 Head pressure reached 0.95psi in 10 seconds @13:42:59.20 Abs.hold @13:42:59.34 PhaseSeparator.in :full @13:42:59.43 PhaseSeparator.hold @13:42:59.68 Waiting for sampler to finish priming... @13:42:59.69 delayUntil "13:44:20.36" @13:44:20.44 SamplePump.in :start @13:44:20.54 SamplePump.seek 50ml,3:50 @13:44:21.12 SamplePump.configure :prime @13:44:35.21 SamplePump.stop @13:44:35.38 Cartridge::Sampler::LostHeadPressure in n -- Head Pressure Fell to 0.28psi @13:44:35.40 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @13:44:35.49 PhaseSeparator.in :filling @13:44:35.56 PhaseSeparator.hold @13:44:35.67 Gate.power :sampler,:OFF @13:44:36.05 Gate.power :sampler,:ON @13:44:51.08 Gate.power :sampler,:OFF @13:44:51.46 Gate.power :sampler,:ON @13:45:06.49 Gate.power :sampler,:OFF @13:45:06.89 Gate.power :sampler,:ON @13:45:21.92 Gate.power :sampler,:OFF @13:45:22.30 Gate.power :sampler,:ON @13:45:37.34 Gate.power :sampler,:OFF @13:45:37.72 Gate.power :sampler,:ON @13:45:52.75 Gate.power :sampler,:OFF @13:45:53.14 Gate.power :sampler,:ON @13:46:08.17 Gate.power :sampler,:OFF @13:46:08.57 Gate.power :sampler,:ON @13:46:23.60 Gate.power :sampler,:OFF @13:46:23.98 Gate.power :sampler,:ON @13:46:36.49 PhaseSeparator.stop @13:46:36.56 Abs.stop @13:46:36.66 Cartridge::Sampler::HeadPressureTooLow in n -- Only reached -0.00psi in 2:01 @13:49:45.63 -> Power.sampler :off @13:49:45.66 Gate.power :sampler,:OFF false @13:51:58.91 -> ESP.shutdown @13:51:59.03 Intake.close @13:51:59.13 Bypass.close @13:51:59.93 Gate.power :analytic1,:OFF @13:51:59.95 Gate.power :motor,:OFF @13:51:59.99 Gate.power :core,:OFF @13:52:00.03 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @13:52:17.42 -> MainThread.exit # @13:52:17.55 Session Ended @10:45:25.32PDT31-Jul-24

Environmental Sample Processor built 01-Jul-24 (3743e013) @10:45:26.93 >BREAK< ESP3 Gateway v1.43 for core bus @10:45:26.96 >BREAK< ESP3 Gateway v1.43 for cartridge bus @10:45:30.44 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @10:45:31.13 Sea pressure (Abs) tare is 6% of full scale @10:45:38.55 Polling Can every 2:00 @10:45:38.60 Waiting for espclients... @10:45:39.66 Session Begun @10:45:57.12 -> Sampler.primeVolume @10:45:57.16 NoMethodError in n -- undefined method `primeVolume' for Cartridge::Sampler:Class @10:46:11.00 -> Cartridge.sampler.primeVolume @10:46:11.04 Cartridge::Error in n -- No Cartridge mounted @10:46:37.28 -> BACsampler.primeVolume=100 100 @10:47:36.16 -> BACsampler.bypassVolume=50 50 Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @10:49:16.96 Session Begun @10:49:22.00 -> ESP.ready! @10:49:22.20 Gate.power :sampler,:OFF,limit to 3500mA @10:49:22.23 Gate.power :skimmer,:OFF,limit to 3500mA @10:49:22.27 Gate.power :analytic1,:ON,limit to 4000mA @10:49:23.00 Plunge.configure :default @10:49:23.15 Plunge.hold @10:49:23.25 Plunge.configure :default @10:49:23.45 SamplePump.configure :default @10:49:23.63 Toroid.configure :default @10:49:23.72 Toroid.hold @10:49:23.84 Toroid.configure :default @10:49:24.02 Carousel.configure :default @10:49:24.13 Carousel.hold @10:49:24.25 Carousel.configure :default @10:49:24.69 Abs.configure :default @10:49:24.87 PhaseSeparator.configure :default @10:49:25.09 Archive.configure :default @10:49:25.28 Air.configure :default @10:49:25.37 Air.hold @10:49:25.49 Air.configure :default @10:49:25.64 Lysis1.configure :default @10:49:25.75 Lysis1.hold @10:49:25.86 Lysis1.configure :default @10:49:26.02 Lysis2.configure :default @10:49:26.46 PinPort.configure :default @10:49:26.55 All elf controllers running firmware version 4.92 @10:49:27.80 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:49:27.84 AnalyticModule::Unknown in n#2 -- SPR @10:49:28.63 Toroid.seek :clear @10:49:28.84 Carousel.in :default @10:49:28.99 Carousel.home.seek 30 @10:49:29.46 Carousel.reconfigure :locked @10:49:29.64 Powering cartridge bus @10:49:32.31 Gate.setIR 1750,5000,0,6 @10:49:32.44 Gate.setIR 0,5000,0,6 @10:49:32.56 Gate.power :cartridge,:OFF @10:49:33.15 Mounted archive Cartridge Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 7.76ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 30 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.16psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) full Lysis1 = Lysis Syringe #1 (LyS1) locked Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:49:42.52 -> BACsampler.primeVolume=100 100 @10:49:50.34 -> BACsampler.bypassVolume=50 50 @10:50:01.06 -> PhytoSampler.bypassVolume=50 50 @10:50:11.31 -> PhytoSampler.primeVolume=100 100 @10:50:14.86 -> BACsampler.xray @10:50:31.29 -> slots {28..26 => { Type: :lyseHold_phyto, State: :dry}, 29 => { Type: :archiveHiBiomass_bac, State: :dry}, 30 => { Type: :archiveHiBiomass_bac, State: :leakyToroid}} @10:50:43.65 -> slot 30=>:dry {30 => { Type: :archiveHiBiomass_bac, State: :dry}} @10:50:49.34 -> Cartridge.lock @10:50:50.31 Powering cartridge bus @10:50:52.98 Gate.setIR 1750,5000,0,6 @10:50:53.11 Gate.setIR 0,5000,0,6 @10:50:53.23 Gate.power :cartridge,:OFF @10:50:53.82 Mounted archive Cartridge :archiveHiBiomass_bac @10:50:59.07 -> Cartridge.sampler.source :sampler @10:51:03.78 -> Cartridge.sampler.source=nil @10:51:08.27 -> Cartridge.unlock @10:51:08.39 Carousel.in :default @10:51:08.56 Carousel.home.seek 30 @10:51:09.01 Air.in :unlocking @10:51:09.25 Air.seek :clear @10:51:10.29 Lysis1.in :unlocking @10:51:10.45 Lysis1.seek :clear @10:51:10.58 Plunge.in :unlocking @10:51:10.69 Plunge.seek :clear @10:51:24.56 -> to 28 @10:51:24.88 Carousel.seek 28 @10:51:25.66 Lysis1.in :fast @10:51:25.76 Lysis1.seek :locked @10:51:28.43 Plunge.in :default @10:51:28.57 Plunge.seek :locked @10:51:28.72 Air.in :fast @10:51:28.82 Air.seek :locked @10:51:29.82 Carousel.reconfigure :locked @10:51:30.02 Powering cartridge bus @10:51:32.68 Gate.setIR 1750,5000,0,6 @10:51:32.81 Gate.setIR 0,5000,0,6 @10:51:32.92 PuckHeater.configure :default @10:51:33.02 Gate.power :heat,:ON,limit to 5000mA @10:51:33.80 Mounted lysis Cartridge board#Q05067 @10:51:33.87 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @10:51:41.22 -> Cartridge.sampler.source=nil @10:51:47.96 -> Cartridge.unlock @10:51:48.03 Gate.power :heat,:OFF @10:51:48.04 Gate.power :cartridge,:OFF @10:51:48.18 Carousel.in :default @10:51:48.34 Carousel.home.seek 28 @10:51:48.80 Air.in :unlocking @10:51:48.91 Air.seek :clear @10:51:49.97 Lysis1.in :unlocking @10:51:50.12 Lysis1.seek :clear @10:51:50.26 Plunge.in :unlocking @10:51:50.36 Plunge.seek :clear @10:52:14.50 -> 2.times{refillSampleLines};fire archive,archive # @10:52:16.06 Toroid.seek :clear @10:52:16.28 Carousel.in :default @10:52:16.44 Carousel.home.seek 28 @10:52:16.73 Selecting Cartridge 30 @10:52:17.10 Carousel.seek 30 @10:52:17.88 Lysis1.in :fast @10:52:17.98 Lysis1.seek :locked @10:52:20.64 Plunge.in :default @10:52:20.73 Plunge.seek :locked @10:52:20.93 Air.in :fast @10:52:21.05 Air.seek :locked @10:52:22.07 Carousel.reconfigure :locked @10:52:22.26 Powering cartridge bus @10:52:24.94 Gate.setIR 1750,5000,0,6 @10:52:25.07 Gate.setIR 0,5000,0,6 @10:52:25.21 Gate.power :cartridge,:OFF @10:52:25.80 Mounted archive Cartridge @10:52:25.93 -> Cmd.stop @10:52:25.99 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 30" @10:52:26.34 Intake.close @10:52:26.44 Exhaust.close @10:52:26.55 Bypass.close @10:52:27.10 Carousel.in :default @10:52:27.16 Cmd::Error in detached from n#2 -- Cannot filter a missing cartridge @10:52:27.25 Waiting to Resume from Checkpoint "Selecting Cartridge 30" @10:52:27.44 Carousel.home.seek 30 @10:52:27.85 Plunge.seek :locked @10:52:28.21 Air.in :unlocking @10:52:28.36 Air.seek :clear @10:52:28.55 Lysis1.in :unlocking @10:52:28.68 Lysis1.seek :clear @10:52:30.19 BadNews.email "Cannot filter a missing cartridge",Subject: "Cmd::Error in detached from n#2" @10:52:30.66 Gate.power :analytic1,:OFF @10:52:30.72 Gate.power :motor,:OFF @10:52:30.79 Gate.power :core,:OFF @10:52:30.84 Gate.power :main,:OFF @10:52:30.91 Safely stopped and ready to power off :STOPPED @10:52:32.82 upsync @10:52:34.88 -> ESP.ready! @10:52:35.07 Gate.power :sampler,:OFF,limit to 3500mA @10:52:35.12 Gate.power :skimmer,:OFF,limit to 3500mA @10:52:35.24 Gate.power :main,:ON,limit to 5000mA @10:52:35.34 Gate.power :analytic1,:ON,limit to 4000mA @10:52:35.49 Powering core bus @10:52:36.90 upsync @10:52:38.30 Plunge.configure :default @10:52:38.42 SamplePump.configure :default @10:52:38.55 Toroid.configure :default @10:52:38.68 Carousel.configure :default @10:52:38.80 Abs.configure :default @10:52:38.93 PhaseSeparator.configure :default @10:52:39.08 Archive.configure :default @10:52:39.47 Air.configure :default @10:52:39.58 Lysis1.configure :default @10:52:39.67 Lysis2.configure :default @10:52:39.80 PinPort.configure :default @10:52:39.89 All elf controllers running firmware version 4.92 @10:52:39.93 Gate.power :motor,:ON,limit to 5000mA @10:52:42.72 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @10:52:42.74 AnalyticModule::Unknown in n#2 -- SPR @10:52:43.31 Plunge.home.jog 0 @10:52:43.78 Carousel.home.jog 0 @10:52:43.88 Lysis2.home.jog 0 @10:52:43.98 Air.home.jog 0 @10:52:44.02 Toroid.home.jog 0 @10:52:44.07 Lysis1.home.jog 0 @10:52:44.13 Archive.home.jog 0 @10:52:44.19 PinPort.home.jog 0 @10:52:45.10 Plunge.in :homing @10:52:45.22 Plunge.jog 1607 @10:52:45.43 PinPort.in :homing @10:52:45.50 Lysis2.in :homing @10:52:45.57 Air.in :homing @10:52:45.62 Toroid.in :homing @10:52:45.67 Lysis1.in :homing @10:52:45.72 Archive.in :homing @10:52:46.02 Lysis2.jog 1407 @10:52:46.10 PinPort.jog -110 @10:52:46.15 Air.jog 1361 @10:52:46.19 Toroid.jog 623 @10:52:46.27 Lysis1.jog 1394 @10:52:46.36 Archive.jog 1392 @10:52:46.78 PinPort.setRawPosition! 0 @10:52:46.80 Plunge.setRawPosition! 50 @10:52:46.96 PinPort.seek :home @10:52:47.00 Plunge.seek :home @10:52:47.28 PinPort.in :default @10:52:47.46 Plunge.in :default @10:52:47.59 Lysis2.setRawPosition! 50 @10:52:47.70 Lysis2.seek :home @10:52:47.87 Lysis1.setRawPosition! 50 @10:52:47.93 Air.setRawPosition! 50 @10:52:48.01 Archive.setRawPosition! 50 @10:52:48.10 Lysis1.seek :home @10:52:48.20 Air.seek :home @10:52:48.35 Archive.seek :home @10:52:48.68 Lysis2.in :default @10:52:49.33 Air.in :default @10:52:49.35 Archive.in :default @10:52:49.41 Lysis1.in :default @10:52:49.46 Toroid.setRawPosition! 0 @10:52:49.72 Toroid.seek :home @10:52:49.95 Toroid.in :default @10:52:50.14 Toroid.seek :clear @10:52:51.86 Carousel.in :default @10:52:52.03 Carousel.home.seek 30 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 30 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:52:55.79 -> require 'vacuumCart' true @10:53:10.20 -> 2.times{refillSampleLines};fire archive,archive @10:53:10.23 Refilling sample & bypass lines with fluid. @10:53:11.10 Toroid.seek :clear @10:53:11.32 Carousel.in :default @10:53:11.48 Carousel.home.seek 30 @10:53:11.76 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @10:53:11.82 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @10:53:11.85 Bypass.open @10:53:12.58 Intake.open @10:53:13.32 SamplePump.setPosition! 0ml @10:53:13.48 SamplePump.seek 30ml @10:53:50.71 Bypass.close @10:53:51.45 Exhaust.open @10:53:52.21 SamplePump.seek 55ml @10:54:23.33 delay 1 second @10:54:24.37 Intake.close @10:54:24.41 Exhaust.close @10:54:25.22 Sampling Loop filled with water @10:54:25.24 Refilling sample & bypass lines with fluid. @10:54:26.07 Toroid.seek :clear @10:54:26.30 Carousel.in :default @10:54:26.46 Carousel.home.seek 30 @10:54:26.76 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @10:54:26.82 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @10:54:26.83 Bypass.open @10:54:27.56 Intake.open @10:54:28.28 SamplePump.setPosition! 0ml @10:54:28.45 SamplePump.seek 30ml @10:55:05.67 Bypass.close @10:55:06.37 Exhaust.open @10:55:07.13 SamplePump.seek 55ml @10:55:38.24 delay 1 second @10:55:39.80 Intake.close @10:55:39.91 Exhaust.close @10:55:40.61 Sampling Loop filled with water # @10:55:41.76 Toroid.seek :clear @10:55:42.00 Carousel.in :default @10:55:42.15 Carousel.home.seek 30 @10:55:42.45 Selecting Cartridge 30 @10:55:42.78 Carousel.seek 30 @10:55:43.10 Lysis1.in :fast @10:55:43.21 Lysis1.seek :locked @10:55:45.88 Plunge.seek :locked @10:55:46.10 Air.in :fast @10:55:46.19 Air.seek :locked @10:55:47.20 Carousel.reconfigure :locked @10:55:47.41 Powering cartridge bus @10:55:50.08 Gate.setIR 1750,5000,0,6 @10:55:50.21 Gate.setIR 0,5000,0,6 @10:55:50.33 Gate.power :cartridge,:OFF @10:55:50.90 Mounted archive Cartridge @10:55:51.04 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 30" @10:55:51.41 Preparing to filter cartridge 30 @10:55:51.77 Priming sample loop w/100ml, bypass w/50ml @10:55:51.82 SamplePump.setPosition! 0ml @10:55:51.92 Bypass.open @10:55:53.43 Intake.open @10:55:54.23 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:55:56.87 Vacuum tare is 51% of full scale at 22C (drifted 0%) @10:55:57.72 Abs.in :default @10:55:57.81 Abs.hold @10:55:58.17 SamplePump.in :start @10:55:58.29 SamplePump.seek 50ml,3:50 @10:55:58.87 SamplePump.configure :prime @10:56:00.90 upsync @10:56:05.11 -> PhytoSampler.bypassVolume=10;PhytoSampler.bypassVolume=10 10 @10:56:18.70 -> BACsampler.bypassVolume=10 10 @10:56:59.71 Abs.stop @10:56:59.81 SamplePump.setPosition! 0ml @10:56:59.90 Exhaust.open @10:57:01.28 Bypass.close @10:57:02.83 Abs.in :default @10:57:02.94 Abs.hold @10:57:03.33 SamplePump.in :start @10:57:03.44 SamplePump.seek 100ml,7:10 @10:57:04.03 SamplePump.configure :prime @10:58:01.62 Session Ended @10:59:05.91 Abs.stop @10:59:06.02 Bypass.open @10:59:06.69 Intake.close @10:59:07.51 Toroid.in :hold @10:59:07.59 Toroid.seek :crack @10:59:09.15 Toroid.seek :cartridge @10:59:10.29 Toroid.in :default @10:59:10.37 Toroid.seek :clear @10:59:12.43 Pressurizing cartridge to 26psi above 17.0psia @10:59:12.45 SamplePump.setPosition! 0ml @10:59:12.54 Intake.open @10:59:13.26 Bypass.close @10:59:13.95 Exhaust.close @10:59:14.72 Abs.in :default @10:59:14.81 Abs.hold @10:59:15.09 SamplePump.in :charge @10:59:15.18 SamplePump.seek 2.3ml,42 seconds @10:59:21.29 Abs.stop @10:59:21.38 Intake.close @10:59:22.14 Pressurized cartridge with 0.162ml to 26.2psi @10:59:22.15 Isolated @31.8psia (14.8psi) @10:59:22.18 Verifying cartridge holds at least 0.65..0.85 of 31.8psia @10:59:22.26 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:59:26.86 Cartridge pressure fell 8% to 29.2psia @10:59:27.00 Sampling 1000.0ml @10:59:27.02 SamplePump.setPosition! 0ml @10:59:27.13 Exhaust.open @10:59:27.79 Intake.open @10:59:29.15 Abs.in :default @10:59:29.23 Abs.hold @10:59:29.67 SamplePump.in :start @10:59:29.77 SamplePump.seek 1000ml,1:23:50 @10:59:30.36 SamplePump.configure :sample @10:59:32.76 Cartridge 30 filtering 1/1000ml at 0.46ml/s with 14.6psi vacuum 7.6m deep (25.9psia) @11:00:32.82 Cartridge 30 filtering 28/1000ml at 0.45ml/s with 28.2psi vacuum @11:01:32.81 Cartridge 30 filtering 54/1000ml at 0.42ml/s with 20.5psi vacuum 3.9m deep (20.5psia) @11:02:32.82 Cartridge 30 filtering 77/1000ml at 0.39ml/s with 20.8psi vacuum 4.1m deep (20.8psia) @11:03:32.81 Cartridge 30 filtering 98/1000ml at 0.36ml/s with 29.7psi vacuum 4.2m deep (20.8psia) @11:04:32.83 Cartridge 30 filtering 118/1000ml at 0.32ml/s with 21.0psi vacuum @11:05:32.84 Cartridge 30 filtering 135/1000ml at 0.29ml/s with 22.7psi vacuum 8.2m deep (26.9psia) @11:06:32.82 Cartridge 30 filtering 151/1000ml at 0.27ml/s with 19.2psi vacuum @11:07:32.82 Cartridge 30 filtering 166/1000ml at 0.24ml/s with 31.3psi vacuum @11:08:32.82 Cartridge 30 filtering 179/1000ml at 0.22ml/s with 22.4psi vacuum 5.8m deep (23.2psia) @11:09:32.82 Cartridge 30 filtering 191/1000ml at 0.20ml/s with 19.4psi vacuum @11:09:32.89 SamplePump.stop @11:09:32.98 Intake.close @11:09:33.13 Exhaust.close @11:09:33.35 Toroid.in :hold @11:09:33.44 Toroid.seek :bypass @11:09:35.12 Toroid.in :default @11:09:35.22 Toroid.seek :clear @11:09:36.86 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @11:09:36.89 Abs.stop @11:09:37.19 Sampled 190.6ml @11:09:37.65 GoodNews.email "After filtering 190.6ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 30" @11:09:38.23 Plunge.seek :reagent @11:09:39.10 Sample loop closed. Ready to Deliver Reagents @11:09:39.13 Archive.seek :sealed @11:09:43.21 upsync @11:09:43.43 Archive.seek :evac @11:09:50.99 -> Cmd.stop @11:09:51.06 Archive.stop @11:09:51.14 Cmd::Error in detached from n#2#2 -- Cannot load cartridge while in PROCESSING state @11:09:51.19 Waiting to Resume from Checkpoint "Selecting Cartridge 30" @11:09:51.28 BadNews.email "Cannot load cartridge while in PROCESSING state",Subject: "Cmd::Error in detached from n#2#2" @11:09:51.56 Carousel.in :default @11:09:51.72 Carousel.home.seek 30 @11:09:52.14 Plunge.seek :locked @11:09:53.17 Archive.in :unlocking @11:09:53.37 Archive.seek :clear @11:09:53.43 Air.in :unlocking @11:09:53.67 Air.seek :clear @11:09:53.88 Lysis1.in :unlocking @11:09:54.04 Lysis1.seek :clear @11:09:56.51 upsync @11:09:56.75 Gate.power :analytic1,:OFF @11:09:56.77 Gate.power :motor,:OFF @11:09:56.80 Gate.power :core,:OFF @11:09:56.84 Gate.power :main,:OFF @11:09:56.89 Safely stopped and ready to power off :STOPPED @11:10:08.80 -> Cartridge.unlock @11:10:08.87 BusOff in n#2 -- core bus was not powered while reading Handoff Pin Port (PP)'s status @11:10:18.87 -> ESP.ready! @11:10:18.93 Gate.power :sampler,:OFF,limit to 3500mA @11:10:18.96 Gate.power :skimmer,:OFF,limit to 3500mA @11:10:18.99 Gate.power :main,:ON,limit to 5000mA @11:10:19.04 Gate.power :analytic1,:ON,limit to 4000mA @11:10:19.17 Powering core bus @11:10:21.95 Plunge.configure :default @11:10:22.06 SamplePump.configure :default @11:10:22.17 Toroid.configure :default @11:10:22.27 Carousel.configure :default @11:10:22.37 Abs.configure :default @11:10:22.48 PhaseSeparator.configure :default @11:10:22.58 Archive.configure :default @11:10:22.69 Air.configure :default @11:10:22.78 Lysis1.configure :default @11:10:22.86 Lysis2.configure :default @11:10:23.23 PinPort.configure :default @11:10:23.31 All elf controllers running firmware version 4.92 @11:10:23.34 Gate.power :motor,:ON,limit to 5000mA @11:10:26.44 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:10:26.47 AnalyticModule::Unknown in n#2 -- SPR @11:10:26.85 Toroid.home.jog 0 @11:10:27.12 Plunge.home.jog 0 @11:10:27.72 PinPort.home.jog 0 @11:10:27.76 Lysis2.home.jog 0 @11:10:27.81 Air.home.jog 0 @11:10:27.85 Archive.home.jog 0 @11:10:27.91 Carousel.home.jog 0 @11:10:27.98 Lysis1.home.jog 0 @11:10:29.28 Plunge.in :homing @11:10:29.36 Toroid.in :homing @11:10:29.60 Plunge.jog 1605 @11:10:29.65 Toroid.jog 621 @11:10:29.90 Lysis2.in :homing @11:10:29.97 Lysis1.in :homing @11:10:30.01 Archive.in :homing @11:10:30.04 PinPort.in :homing @11:10:30.11 Air.in :homing @11:10:30.30 Lysis2.jog 1405 @11:10:30.38 Lysis1.jog 1395 @11:10:30.43 Archive.jog 1401 @11:10:30.47 PinPort.jog -109 @11:10:30.58 Air.jog 1364 @11:10:30.96 Plunge.setRawPosition! 50 @11:10:31.07 Plunge.seek :home @11:10:31.20 PinPort.setRawPosition! 0 @11:10:31.27 PinPort.seek :home @11:10:31.41 Plunge.in :default @11:10:31.50 PinPort.in :default @11:10:31.75 Lysis2.setRawPosition! 50 @11:10:31.84 Lysis2.seek :home @11:10:32.00 Air.setRawPosition! 50 @11:10:32.05 Archive.setRawPosition! 50 @11:10:32.09 Toroid.setRawPosition! 0 @11:10:32.16 Lysis1.setRawPosition! 50 @11:10:32.32 Air.seek :home @11:10:32.39 Archive.seek :home @11:10:32.46 Toroid.seek :home @11:10:32.51 Lysis1.seek :home @11:10:32.92 Air.in :default @11:10:32.97 Archive.in :default @11:10:33.01 Lysis2.in :default @11:10:33.08 Toroid.in :default @11:10:33.35 Lysis1.in :default @11:10:33.70 Toroid.seek :clear @11:10:35.39 Carousel.in :default @11:10:35.54 Carousel.home.seek 30 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 30 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:11:12.13 -> ESP.shutdown @11:11:12.32 Gate.power :analytic1,:OFF @11:11:12.34 Gate.power :motor,:OFF @11:11:12.37 Gate.power :core,:OFF @11:11:12.41 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @11:24:01.46 -> slot {28..26 => { Type: :lyseHold_phyto, State: :dry}, 29 => { Type: :archiveHiBiomass_bac, State: :dry}, 30 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 190.6}} @11:24:59.41 -> slot [29..28] =>nil {[29,28] => nil} @11:25:02.63 -> slot {[27,26] => { Type: :lyseHold_phyto, State: :dry}, 30 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 190.6}} @11:25:28.48 -> reqiure 'vacuumCart' @11:25:28.51 NoMethodError in n#2 -- undefined method `reqiure' for CmdServer::Client:Class @11:25:35.39 -> ESP.configure @11:25:35.44 Gate.power :sampler,:OFF,limit to 3500mA @11:25:35.47 Gate.power :skimmer,:OFF,limit to 3500mA @11:25:35.50 Gate.power :main,:ON,limit to 5000mA @11:25:35.54 Gate.power :analytic1,:ON,limit to 4000mA @11:25:35.64 Powering core bus @11:25:38.39 Plunge.configure :default @11:25:38.55 SamplePump.configure :default @11:25:38.70 Toroid.configure :default @11:25:38.81 Carousel.configure :default @11:25:38.92 Abs.configure :default @11:25:39.02 PhaseSeparator.configure :default @11:25:39.15 Archive.configure :default @11:25:39.25 Air.configure :default @11:25:39.33 Lysis1.configure :default @11:25:39.67 Lysis2.configure :default @11:25:39.77 PinPort.configure :default @11:25:39.84 All elf controllers running firmware version 4.92 @11:25:39.87 Gate.power :motor,:ON,limit to 5000mA @11:25:42.91 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @11:25:42.94 AnalyticModule::Unknown in n#2 -- SPR @11:25:48.02 -> reqiure 'vacuumCart' @11:25:48.05 NoMethodError in n#2 -- undefined method `reqiure' for CmdServer::Client:Class @11:26:00.19 -> require 'bvacuumCart' @11:26:00.77 LoadError in n#2 -- no such file to load -- bvacuumCart @11:26:08.88 -> require 'vacuumCart' false @11:26:13.74 -> require 'vacuumCart' false @11:26:22.21 -> ESP.ready! @11:26:22.38 Plunge.home.jog 0 @11:26:23.25 PinPort.home.jog 0 @11:26:23.30 Lysis1.home.jog 0 @11:26:23.35 Archive.home.jog 0 @11:26:23.39 Toroid.home.jog 0 @11:26:23.44 Lysis2.home.jog 0 @11:26:23.49 Air.home.jog 0 @11:26:23.52 Carousel.home.jog 0 @11:26:24.45 Plunge.in :homing @11:26:24.71 Plunge.jog 153 @11:26:24.79 Lysis2.in :homing @11:26:24.83 Air.in :homing @11:26:24.89 Toroid.in :homing @11:26:24.94 PinPort.in :homing @11:26:24.99 Lysis1.in :homing @11:26:25.04 Archive.in :homing @11:26:25.44 Lysis2.jog 1404 @11:26:25.48 PinPort.jog -110 @11:26:25.53 Toroid.jog 618 @11:26:25.59 Air.jog 1357 @11:26:25.63 Lysis1.jog 1392 @11:26:25.68 Archive.jog 1388 @11:26:26.15 PinPort.setRawPosition! 0 @11:26:26.17 Plunge.setRawPosition! 50 @11:26:26.32 PinPort.seek :home @11:26:26.35 Plunge.seek :home @11:26:26.55 PinPort.in :default @11:26:26.73 Plunge.in :default @11:26:26.91 Lysis2.setRawPosition! 50 @11:26:27.00 Lysis2.seek :home @11:26:27.18 Lysis1.setRawPosition! 50 @11:26:27.20 Air.setRawPosition! 50 @11:26:27.25 Archive.setRawPosition! 50 @11:26:27.43 Lysis1.seek :home @11:26:27.48 Air.seek :home @11:26:27.57 Archive.seek :home @11:26:27.82 Lysis2.in :default @11:26:27.96 Lysis1.in :default @11:26:28.09 Air.in :default @11:26:28.13 Archive.in :default @11:26:28.21 Toroid.setRawPosition! 0 @11:26:28.34 Toroid.seek :home @11:26:28.46 Toroid.in :default @11:26:28.61 Toroid.seek :clear @11:26:30.28 Carousel.in :default @11:26:30.43 Carousel.home.seek 30 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 30 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:26:42.41 -> require 'vacuumCart' false @11:26:51.79 -> require 'vacuumCarts' @11:26:51.84 LoadError in n#2 -- no such file to load -- vacuumCarts @11:26:56.28 -> require 'vacuumCart' false @11:27:02.88 -> vacToroid @11:27:02.91 Vacuuming protocol for dry cartridges @11:27:03.71 Toroid.seek :clear @11:27:03.92 Carousel.in :default @11:27:04.06 Carousel.home.seek 30 @11:27:04.35 Waiting for n#2 to answer "Disconnect the intake line. push return" @11:27:07.50 Answered Disconnect the intake line. push return"" @11:27:08.30 Toroid.seek :clear @11:27:08.49 Carousel.in :default @11:27:08.63 Carousel.home.seek 30 @11:27:08.91 SamplePump.setPosition! 0ml @11:27:08.97 Intake.open @11:27:09.67 Exhaust.open @11:27:10.35 SamplePump.seek 50ml @11:28:11.98 Exhaust.close @11:28:12.65 Bypass.open @11:28:13.35 SamplePump.seek 75ml @11:28:44.47 Intake.close @11:28:45.14 Bypass.close @11:28:45.81 Waiting for n#2 to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @11:28:51.73 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @11:28:51.75 Exhaust.open @11:28:52.46 Bypass.open @11:28:53.14 Waiting for n#2 to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @11:30:25.84 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @11:30:25.87 delay 30 seconds @11:30:55.92 Clearing fluid from cartridge-side of Toroid @11:30:56.23 Carousel.seek 26 @11:30:57.27 Toroid.in :hold @11:30:57.35 Toroid.seek :cartridge @11:30:59.21 Toroid.in :default @11:30:59.30 Toroid.seek :clear @11:31:01.14 delay 2 seconds @11:31:03.25 Toroid.in :hold @11:31:03.34 Toroid.seek :bypass @11:31:05.01 Toroid.in :default @11:31:05.14 Toroid.seek :clear @11:31:07.52 Carousel.seek 27 @11:31:08.05 Toroid.in :hold @11:31:08.13 Toroid.seek :cartridge @11:31:10.00 Toroid.in :default @11:31:10.08 Toroid.seek :clear @11:31:11.91 delay 2 seconds @11:31:14.03 Toroid.in :hold @11:31:14.12 Toroid.seek :bypass @11:31:15.78 Toroid.in :default @11:31:15.86 Toroid.seek :clear @11:31:17.50 Waiting for n#2 to answer "Turn the pump off & load the cartridges. Press Return when loaded." @11:32:28.92 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @11:32:29.69 Toroid.seek :clear @11:32:29.88 Carousel.in :default @11:32:30.02 Carousel.home.seek 27 @11:32:30.31 Waiting for n#2 to answer "Turn the pump on. Push Return" @11:32:34.57 Answered Turn the pump on. Push Return"" @11:32:34.59 Vacuuming individual cartridges. @11:32:35.45 Toroid.seek :clear @11:32:35.66 Carousel.in :default @11:32:35.80 Carousel.home.seek 27 @11:32:36.35 Carousel.seek 26 @11:32:36.91 Lysis1.in :fast @11:32:36.97 Lysis1.seek :locked @11:32:39.66 Plunge.seek :locked @11:32:39.85 Air.in :fast @11:32:39.93 Air.seek :locked @11:32:40.92 Carousel.reconfigure :locked @11:32:41.13 Powering cartridge bus @11:32:43.80 Gate.setIR 1750,5000,0,6 @11:32:43.94 Gate.setIR 0,5000,0,6 @11:32:44.03 PuckHeater.configure :default @11:32:44.11 Gate.power :heat,:ON,limit to 5000mA @11:32:45.31 Mounted lysis Cartridge board#Q05087 @11:32:45.46 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:32:45.50 Vacuuming Vent Top cartridge 26 for 30 seconds @11:32:45.54 Air.in :default @11:32:45.64 Air.seek :evac @11:33:00.21 Toroid.in :hold @11:33:00.29 Toroid.seek :cartridge @11:33:02.15 Toroid.in :default @11:33:02.23 Toroid.seek :clear @11:33:04.07 Polling Can every 2:00 @11:33:04.62 PuckHeater.configure :default #ambient 21.4C @11:33:04.71 PuckHeater.seek 41.1C,1:00 @11:33:26.24 PuckHeater.hold 3 seconds,41.1C @11:33:29.47 delayUntil "11:33:33.31" @11:33:33.41 Toroid.in :hold @11:33:33.50 Toroid.seek :bypass @11:33:35.16 Toroid.in :default @11:33:35.25 Toroid.seek :clear @11:33:36.89 delay 2 seconds @11:33:38.92 Air.seek :full @11:33:53.55 Gate.power :heat,:OFF @11:33:53.58 Gate.power :cartridge,:OFF @11:33:53.69 Carousel.in :default @11:33:53.82 Carousel.home.seek 26 @11:33:54.27 Air.in :unlocking @11:33:54.40 Air.seek :clear @11:33:55.43 Lysis1.in :unlocking @11:33:55.59 Lysis1.seek :clear @11:33:55.68 Plunge.in :unlocking @11:33:55.78 Plunge.seek :clear @11:33:57.00 Carousel.seek 27 @11:33:57.61 Lysis1.in :fast @11:33:57.69 Lysis1.seek :locked @11:34:00.81 Plunge.in :default @11:34:00.99 Plunge.seek :locked @11:34:01.22 Air.in :fast @11:34:01.30 Air.seek :locked @11:34:02.29 Carousel.reconfigure :locked @11:34:02.47 Powering cartridge bus @11:34:05.17 Gate.setIR 1750,5000,0,6 @11:34:05.31 Gate.setIR 0,5000,0,6 @11:34:05.43 PuckHeater.configure :default #ambient 21.4C @11:34:05.52 Gate.power :heat,:ON,limit to 5000mA @11:34:06.23 Mounted lysis Cartridge board#Q05049 @11:34:06.28 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:34:06.36 Vacuuming Vent Top cartridge 27 for 30 seconds @11:34:06.40 Air.in :default @11:34:06.48 Air.seek :evac @11:34:21.08 Toroid.in :hold @11:34:21.22 Toroid.seek :cartridge @11:34:23.12 Toroid.in :default @11:34:23.20 Toroid.seek :clear @11:34:25.07 Polling Can every 2:00 @11:34:25.62 PuckHeater.configure :default #ambient 21.4C @11:34:25.71 PuckHeater.seek 41.1C,1:00 @11:34:47.27 PuckHeater.hold 3 seconds,41.1C @11:34:50.48 delayUntil "11:34:54.24" @11:34:54.35 Toroid.in :hold @11:34:54.43 Toroid.seek :bypass @11:34:56.10 Toroid.in :default @11:34:56.18 Toroid.seek :clear @11:34:57.82 delay 2 seconds @11:34:59.85 Air.seek :full @11:35:14.34 Gate.power :heat,:OFF @11:35:14.36 Gate.power :cartridge,:OFF @11:35:14.48 Carousel.in :default @11:35:14.62 Carousel.home.seek 27 @11:35:15.11 Air.in :unlocking @11:35:15.22 Air.seek :clear @11:35:16.81 Lysis1.in :unlocking @11:35:16.96 Lysis1.seek :clear @11:35:17.12 Plunge.in :unlocking @11:35:17.20 Plunge.seek :clear @11:35:18.34 Cartridges under vacuum. Individual Leak Test begins @11:35:18.37 Waiting for n#2 to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @11:35:33.76 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @11:35:33.79 delay 10:00 @11:45:33.84 Waiting for n#2 to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @11:46:33.01 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @11:46:33.07 move through each cartridge position & record end vacuum @11:46:33.10 Vacuuming cartridge 26 @11:46:33.42 Carousel.seek 26 @11:46:33.97 Lysis1.in :fast @11:46:34.06 Lysis1.seek :locked @11:46:36.72 Plunge.in :default @11:46:36.85 Plunge.seek :locked @11:46:36.98 Air.in :fast @11:46:37.09 Air.seek :locked @11:46:38.09 Carousel.reconfigure :locked @11:46:38.28 Powering cartridge bus @11:46:40.95 Gate.setIR 1750,5000,0,6 @11:46:41.09 Gate.setIR 0,5000,0,6 @11:46:41.18 PuckHeater.configure :default #ambient 21.4C @11:46:41.27 Gate.power :heat,:ON,limit to 5000mA @11:46:41.98 Mounted lysis Cartridge board#Q05087 @11:46:42.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:46:42.19 Toroid.in :hold @11:46:42.27 Toroid.seek :cartridge @11:46:44.13 Toroid.in :default @11:46:44.22 Toroid.seek :clear @11:46:46.07 Waiting for n#2 to answer "record Vacuum" @11:46:59.84 Answered record Vacuum"" @11:46:59.97 Toroid.in :hold @11:47:00.07 Toroid.seek :bypass @11:47:01.74 Toroid.in :default @11:47:01.84 Toroid.seek :clear @11:47:03.48 Waiting for n#2 to answer "recharge if needed" @11:47:11.20 Answered recharge if needed"" @11:47:11.22 Vacuuming cartridge 27 @11:47:11.43 Gate.power :heat,:OFF @11:47:11.46 Gate.power :cartridge,:OFF @11:47:11.57 Carousel.in :default @11:47:11.71 Carousel.home.seek 26 @11:47:12.14 Air.in :unlocking @11:47:12.28 Air.seek :clear @11:47:13.30 Lysis1.in :unlocking @11:47:13.45 Lysis1.seek :clear @11:47:14.18 Plunge.in :unlocking @11:47:14.28 Plunge.seek :clear @11:47:15.41 Carousel.seek 27 @11:47:15.97 Lysis1.in :fast @11:47:16.05 Lysis1.seek :locked @11:47:18.70 Plunge.in :default @11:47:18.79 Plunge.seek :locked @11:47:18.98 Air.in :fast @11:47:19.07 Air.seek :locked @11:47:20.07 Carousel.reconfigure :locked @11:47:20.25 Powering cartridge bus @11:47:22.92 Gate.setIR 1750,5000,0,6 @11:47:23.07 Gate.setIR 0,5000,0,6 @11:47:23.16 PuckHeater.configure :default #ambient 21.4C @11:47:23.26 Gate.power :heat,:ON,limit to 5000mA @11:47:24.03 Mounted lysis Cartridge board#Q05049 @11:47:24.08 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:47:24.23 Toroid.in :hold @11:47:24.31 Toroid.seek :cartridge @11:47:26.17 Toroid.in :default @11:47:26.26 Toroid.seek :clear @11:47:28.09 Waiting for n#2 to answer "record Vacuum" @11:47:43.25 Answered record Vacuum"" @11:47:43.39 Toroid.in :hold @11:47:43.48 Toroid.seek :bypass @11:47:45.16 Toroid.in :default @11:47:45.24 Toroid.seek :clear @11:47:46.88 Waiting for n#2 to answer "recharge if needed" @11:47:48.68 Answered recharge if needed"" @11:47:48.73 Gate.power :heat,:OFF @11:47:48.75 Gate.power :cartridge,:OFF @11:47:48.86 Carousel.in :default @11:47:49.00 Carousel.home.seek 27 @11:47:49.46 Air.in :unlocking @11:47:49.62 Air.seek :clear @11:47:50.66 Lysis1.in :unlocking @11:47:50.79 Lysis1.seek :clear @11:47:50.93 Plunge.in :unlocking @11:47:51.00 Plunge.seek :clear @11:47:52.18 Waiting for n#2 to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @11:47:57.85 UserAbort in n#2 -- User Abort!! @11:49:09.73 -> refillSampleLines @11:49:09.76 Refilling sample & bypass lines with fluid. @11:49:10.58 Toroid.seek :clear @11:49:11.32 Carousel.in :default @11:49:11.55 Carousel.home.seek 27 @11:49:11.84 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @11:49:15.01 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @11:49:15.02 Intake.open @11:49:15.76 SamplePump.setPosition! 0ml @11:49:15.91 SamplePump.seek 30ml @11:49:53.13 Bypass.close @11:49:53.99 SamplePump.seek 55ml @11:50:25.09 delay 1 second @11:50:26.13 Intake.close @11:50:26.22 Exhaust.close @11:50:26.98 Sampling Loop filled with water @11:50:36.61 -> refillSampleLines @11:50:36.64 Refilling sample & bypass lines with fluid. @11:50:37.49 Toroid.seek :clear @11:50:37.68 Carousel.in :default @11:50:37.82 Carousel.home.seek 27 @11:50:38.11 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @11:50:41.32 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @11:50:41.34 Bypass.open @11:50:42.03 Intake.open @11:50:42.72 SamplePump.setPosition! 0ml @11:50:42.86 SamplePump.seek 30ml @11:51:20.06 Bypass.close @11:51:20.76 Exhaust.open @11:51:21.48 SamplePump.seek 55ml @11:51:52.58 delay 1 second @11:51:53.61 Intake.close @11:51:53.73 Exhaust.close @11:51:54.48 Sampling Loop filled with water @11:58:09.01 -> refillSampleLines @11:58:09.09 Refilling sample & bypass lines with fluid. @11:58:09.93 Toroid.seek :clear @11:58:10.13 Carousel.in :default @11:58:10.26 Carousel.home.seek 27 @11:58:10.56 Waiting for n#2 to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @11:58:46.08 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @11:58:46.10 Bypass.open @11:58:46.80 Intake.open @11:58:47.51 SamplePump.setPosition! 0ml @11:58:47.64 SamplePump.seek 30ml @11:59:24.84 Bypass.close @11:59:25.53 Exhaust.open @11:59:26.26 SamplePump.seek 55ml @11:59:57.95 delay 1 second @11:59:59.00 Intake.close @11:59:59.13 Exhaust.close @11:59:59.82 Sampling Loop filled with water @12:04:36.50 -> slot {[27,26] => { Type: :lyseHold_phyto, State: :dry}, 30 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 190.6}} @12:04:53.24 -> slot [60..1] =>nil {60..1 => nil} @12:05:25.23 -> slot [28..26] =>[:dry, :microSPR_phyto] {28..26 => { Type: :microSPR_phyto, State: :dry}} @12:05:39.00 -> PhytoSampler PhytoSampler @12:05:50.52 -> PhytoSampler.sampler @12:05:50.56 NoMethodError in n#2 -- undefined method `sampler' for PhytoSampler:Cartridge::Sampler @12:06:07.48 -> PhytoSampler.goalVolume 50 @12:06:23.30 -> PhytoSampler.goalVolumn=25 @12:06:23.35 NoMethodError in n#2 -- undefined method `goalVolumn=' for PhytoSampler:Cartridge::Sampler @12:06:35.33 -> PhytoSampler.goalVolume=25 25 @12:10:39.88 -> ESP.reready! @12:10:39.94 Plunge.in :default @12:10:40.03 Toroid.in :default @12:10:40.13 Carousel.in :default @12:10:40.23 Archive.in :default @12:10:40.37 Plunge.home.jog 0 @12:10:40.41 Air.in :default @12:10:40.48 Lysis1.in :default @12:10:40.56 Lysis2.in :default @12:10:40.60 Toroid.home.jog 0 @12:10:40.63 PinPort.in :default @12:10:40.80 Carousel.home.jog 0 @12:10:41.05 Archive.home.jog 0 @12:10:41.20 Air.home.jog 0 @12:10:41.37 Lysis1.home.jog 0 @12:10:41.49 Lysis2.home.jog 0 @12:10:41.81 PinPort.home.jog 0 @12:10:42.29 Plunge.in :homing @12:10:42.71 Plunge.jog 166 @12:10:42.79 PinPort.in :homing @12:10:42.84 Lysis1.in :homing @12:10:42.88 Archive.in :homing @12:10:42.92 Air.in :homing @12:10:42.97 Toroid.in :homing @12:10:43.01 Lysis2.in :homing @12:10:43.40 Lysis1.jog 1395 @12:10:43.43 PinPort.jog -111 @12:10:43.50 Air.jog 1370 @12:10:43.53 Archive.jog 1391 @12:10:43.58 Lysis2.jog 1403 @12:10:43.62 Toroid.jog 612 @12:10:44.07 PinPort.setRawPosition! 0 @12:10:44.09 Plunge.setRawPosition! 50 @12:10:44.21 PinPort.seek :home @12:10:44.27 Plunge.seek :home @12:10:44.50 PinPort.in :default @12:10:44.64 Plunge.in :default @12:10:44.85 Lysis1.setRawPosition! 50 @12:10:44.95 Lysis1.seek :home @12:10:45.16 Air.setRawPosition! 50 @12:10:45.18 Archive.setRawPosition! 50 @12:10:45.22 Lysis2.setRawPosition! 50 @12:10:45.37 Air.seek :home @12:10:45.44 Archive.seek :home @12:10:45.50 Lysis2.seek :home @12:10:45.79 Lysis1.in :default @12:10:45.91 Air.in :default @12:10:46.05 Archive.in :default @12:10:46.08 Lysis2.in :default @12:10:46.24 Toroid.setRawPosition! 0 @12:10:46.31 Toroid.seek :home @12:10:47.08 Toroid.in :default @12:10:47.21 Toroid.seek :clear @12:10:48.91 Carousel.in :default @12:10:49.09 Carousel.home.seek 27 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 55ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 27 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:11:05.49 -> ESP.ready! @12:11:06.30 Toroid.seek :clear @12:11:06.49 Carousel.in :default @12:11:06.63 Carousel.home.seek 27 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 55ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 27 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:11:28.19 -> exit @12:11:28.22 Session Ended @12:33:38.97PDT31-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:33:40.68 >BREAK< ESP3 Gateway v1.43 for core bus @12:33:40.71 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:33:44.28 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @12:33:44.98 Sea pressure (Abs) tare is 6% of full scale @12:33:53.00 Polling Can every 2:00 @12:33:53.09 Waiting for espclients... @12:34:11.24 Session Begun @12:34:20.13 -> MainThread.exit # @12:34:20.26 ThreadError in bill -- killed thread @12:34:20.36 Session Ended @12:35:11.46PDT31-Jul-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:35:14.60 >BREAK< ESP3 Gateway v1.43 for core bus @12:35:14.63 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:35:18.12 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @12:35:18.82 Sea pressure (Abs) tare is 6% of full scale @12:35:26.44 Polling Can every 2:00 @12:35:26.49 Waiting for espclients... @12:35:26.68 Session Begun @12:35:34.51 -> slot {28..26 => { Type: :microSPR_phyto, State: :dry}} @12:36:55.73 -> PhytoSampler.xray @12:37:16.41 -> PhytoSampler.primeVolume=10 10 @12:37:35.46 -> PhytoSampler.bypassVolume=100 100 @12:38:04.17 -> PhytoSampler.bypassVolume=10 10 @12:38:42.27 -> PhytoSampler.primeVolume=100 100 @12:38:55.46 -> PhytoSampler.goalVolume=25 25 @12:39:04.75 -> SC @12:39:04.81 BusOff in bill -- core bus was not powered while reading Storage Carousel (SC)'s status @12:39:13.79 -> ESP.ready! @12:39:13.85 Gate.power :sampler,:OFF,limit to 3500mA @12:39:13.87 Gate.power :skimmer,:OFF,limit to 3500mA @12:39:13.90 Gate.power :main,:ON,limit to 5000mA @12:39:13.94 Gate.power :analytic1,:ON,limit to 4000mA @12:39:14.13 Powering core bus @12:39:16.40 SPR Module 1, FW Rev. B, FPGA Rev. C @12:39:16.91 Plunge.configure :default @12:39:17.02 SamplePump.configure :default @12:39:17.13 Toroid.configure :default @12:39:17.24 Carousel.configure :default @12:39:17.34 Abs.configure :default @12:39:17.45 PhaseSeparator.configure :default @12:39:17.55 Archive.configure :default @12:39:17.66 Air.configure :default @12:39:17.73 Lysis1.configure :default @12:39:17.80 Lysis2.configure :default @12:39:17.90 PinPort.configure :default @12:39:17.97 All elf controllers running firmware version 4.92 @12:39:18.01 Gate.power :motor,:ON,limit to 5000mA @12:39:18.39 Plunge.home.jog 0 @12:39:19.06 Lysis2.home.jog 0 @12:39:19.12 Air.home.jog 0 @12:39:19.15 Carousel.home.jog 0 @12:39:19.20 Lysis1.home.jog 0 @12:39:19.25 Archive.home.jog 0 @12:39:19.29 PinPort.home.jog 0 @12:39:19.32 Toroid.home.jog 0 @12:39:20.14 Plunge.in :homing @12:39:20.46 Plunge.jog 1594 @12:39:20.56 Lysis1.in :homing @12:39:20.60 Archive.in :homing @12:39:20.65 Lysis2.in :homing @12:39:20.70 Toroid.in :homing @12:39:20.74 PinPort.in :homing @12:39:20.77 Air.in :homing @12:39:21.08 Lysis1.jog 1395 @12:39:21.22 Archive.jog 1391 @12:39:21.27 PinPort.jog -111 @12:39:21.31 Air.jog 1369 @12:39:21.35 Toroid.jog 616 @12:39:21.43 Lysis2.jog 1406 @12:39:21.83 PinPort.setRawPosition! 0 @12:39:21.91 PinPort.seek :home @12:39:22.02 PinPort.in :default @12:39:22.12 Plunge.setRawPosition! 50 @12:39:22.20 Plunge.seek :home @12:39:22.51 Plunge.in :default @12:39:22.64 Lysis1.setRawPosition! 50 @12:39:22.73 Lysis1.seek :home @12:39:22.87 Air.setRawPosition! 50 @12:39:22.90 Archive.setRawPosition! 50 @12:39:23.02 Air.seek :home @12:39:23.07 Archive.seek :home @12:39:23.30 Lysis1.in :default @12:39:23.33 Lysis2.setRawPosition! 50 @12:39:23.49 Lysis2.seek :home @12:39:23.63 Archive.in :default @12:39:23.66 Air.in :default @12:39:24.26 Toroid.setRawPosition! 0 @12:39:24.28 Lysis2.in :default @12:39:24.44 Toroid.seek :home @12:39:24.61 Toroid.in :default @12:39:24.82 Toroid.seek :clear @12:39:26.51 Carousel.in :default @12:39:26.66 Carousel.home.seek 28 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 28 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:39:31.07 -> SC Storage Carousel (SC) at 28 @12:39:43.20 -> PhytoSampler.xray @12:40:22.78 -> PhytoSampler.source = nil @12:40:36.42 -> fire lysis # @12:40:37.40 Toroid.seek :clear @12:40:37.61 Carousel.in :default @12:40:37.75 Carousel.home.seek 28 @12:40:38.06 Selecting Cartridge 28 @12:40:38.43 Carousel.seek 28 @12:40:38.71 Lysis1.in :fast @12:40:38.81 Lysis1.seek :locked @12:40:41.44 Plunge.seek :locked @12:40:41.65 Air.in :fast @12:40:41.73 Air.seek :locked @12:40:42.74 Carousel.reconfigure :locked @12:40:42.92 Powering cartridge bus @12:40:45.59 Gate.setIR 1750,5000,0,6 @12:40:45.72 Gate.setIR 0,5000,0,6 @12:40:45.81 PuckHeater.configure :default @12:40:45.91 Gate.power :heat,:ON,limit to 5000mA @12:40:46.67 Mounted lysis Cartridge board#Q05067 @12:40:46.73 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:40:46.86 GoodNews.email "PhytoSampler filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 28" @12:40:47.22 Preparing to filter cartridge 28 @12:40:48.11 Air.in :default @12:40:48.24 Air.seek :sealed @12:40:48.48 Priming sample loop w/100ml, bypass w/10ml @12:40:48.52 SamplePump.setPosition! 0ml @12:40:48.61 Bypass.open @12:40:50.12 Intake.open @12:40:50.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:40:52.76 upsync @12:40:55.12 Vacuum tare is 52% of full scale at 22C (drifted 1%) @12:40:55.32 Abs.in :default @12:40:55.39 Abs.hold @12:40:55.68 SamplePump.in :start @12:40:55.78 SamplePump.seek 10ml,1:10 @12:40:56.39 SamplePump.configure :prime @12:41:08.37 Abs.stop @12:41:08.48 SamplePump.setPosition! 0ml @12:41:08.56 Exhaust.open @12:41:09.98 Bypass.close @12:41:11.55 Abs.in :default @12:41:11.65 Abs.hold @12:41:11.99 SamplePump.in :start @12:41:12.09 SamplePump.seek 100ml,7:10 @12:41:12.69 SamplePump.configure :prime @12:43:14.54 Abs.stop @12:43:14.65 Bypass.open @12:43:15.38 Intake.close @12:43:16.21 Toroid.in :hold @12:43:16.29 Toroid.seek :crack @12:43:17.91 Toroid.seek :cartridge @12:43:19.06 Toroid.in :default @12:43:19.15 Toroid.seek :clear @12:43:21.23 Pressurizing cartridge to 26psi above 14.7psia @12:43:21.25 SamplePump.setPosition! 0ml @12:43:21.35 Intake.open @12:43:22.05 Bypass.close @12:43:23.28 Exhaust.close @12:43:24.08 Abs.in :default @12:43:24.17 Abs.hold @12:43:24.44 SamplePump.in :charge @12:43:24.61 SamplePump.seek 2.3ml,42 seconds @12:43:39.26 Abs.stop @12:43:39.36 Intake.close @12:43:40.12 Pressurized cartridge with 0.964ml to 26.2psi @12:43:40.14 Isolated @39.0psia (24.3psi) @12:43:40.18 Verifying cartridge holds at least 0.65..0.85 of 39.0psia @12:43:40.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:43:44.85 Cartridge pressure fell 8% to 35.7psia @12:43:44.98 Sampling 25.0ml @12:43:45.00 SamplePump.setPosition! 0ml @12:43:45.09 Exhaust.open @12:43:45.79 Intake.open @12:43:46.70 Abs.in :default @12:43:46.78 Abs.hold @12:43:47.14 SamplePump.in :start @12:43:47.23 SamplePump.seek 25ml,2:35 @12:43:47.83 SamplePump.configure :sample @12:43:50.24 Cartridge 28 filtering 2/25ml at 0.61ml/s with 21.3psi vacuum @12:44:24.01 Abs.stop @12:44:24.19 Intake.close @12:44:24.24 Exhaust.close @12:44:24.58 Toroid.in :hold @12:44:24.66 Toroid.seek :bypass @12:44:26.32 Toroid.in :default @12:44:26.45 Toroid.seek :clear @12:44:28.10 Sampled 25.0ml @12:44:28.63 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 28" @12:44:30.74 SPR.command "read_sequences" @12:44:31.23 SPR.command "stats_on" @12:44:31.49 SPR.command "start 0 1" @12:44:32.12 SPR.start /var/log/esp/spr24jul3112h.spr.bz2 @12:44:34.08 Handoff.select :waste @12:44:34.27 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:44:34.44 upsync @12:44:41.40 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:44:41.44 PinPort.in :locking @12:44:41.58 PinPort.seek :locked @12:44:42.14 Archive.in :fast @12:44:42.19 Lysis2.in :fast @12:44:42.27 Lysis1.seek 1350uL @12:44:42.47 Lysis2.seek 950uL @12:44:42.55 Archive.seek :full @12:44:47.42 Plunge.seek :reagent @12:44:48.37 Evacuating puck with filter bubble point of 24psi @12:44:48.43 PinPort.seek :locked @12:44:48.57 Handoff.select :waste @12:44:48.62 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:44:55.22 Air syringe pumping up to 1 strokes to reach 8psi @12:44:55.24 Air stroke #1 of 1 @12:44:55.37 Air.in :pressurize @12:44:55.45 Air.seek :pressurized @12:45:01.15 1.3psi after pressurize @12:45:01.18 Air.in :compress @12:45:01.28 Air.seek :evac @12:45:04.33 2.1psi after compress @12:45:28.94 0.1psi after compress (stable) @12:45:28.96 Air.in :default @12:45:29.06 Air.hold @12:45:29.31 at -0.2psi Opened to waste @12:45:29.33 Handoff.select :blocked @12:45:29.55 Archive syringe pumping up to 8 strokes to reach 24psi @12:45:29.57 Archive stroke #1 of 8 @12:45:29.69 Archive.in :pressurize @12:45:29.77 Archive.seek :pressurized @12:45:42.23 3.7psi after pressurize @12:46:02.33 8.2psi after pressurize (stable) @12:46:02.40 Archive.in :compress @12:46:02.51 Archive.seek :empty @12:46:18.28 14.6psi after compress @12:46:28.87 13.7psi after compress (stable) @12:46:28.91 Archive.in :gulp @12:46:29.03 Archive.seek :topVent @12:46:35.37 14.6psi after gulp @12:46:41.03 13.8psi after gulp (stable) @12:46:41.05 Archive stroke #2 of 8 @12:46:41.19 Archive.in :pressurize @12:46:41.28 Archive.seek :pressurized @12:46:53.77 14.2psi after pressurize @12:47:46.86 18.5psi after pressurize (stable) @12:47:46.89 Archive.in :compress @12:47:47.00 Archive.seek :empty @12:47:59.05 Reached 24.2 of 24.0psi after pumping 3380uL (1.95 strokes) @12:47:59.08 Archive.in :default @12:47:59.19 Archive.hold @12:47:59.29 Handoff.select :waste @12:47:59.47 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:48:12.09 delay 1:00 @12:49:12.13 Handoff.select :blocked @12:49:12.29 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:49:34.89 Handoff.select :waste @12:49:35.04 0.5psi at evacEnd @12:49:35.06 Handoff.select :blocked @12:49:35.23 Archive.in :fast @12:49:35.34 Archive.seek :topVent @12:49:42.27 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @12:49:48.86 Stable @0.3psi after Handoff blocked @12:49:48.90 Archive syringe pumping up to 8 strokes to reach 24psi @12:49:48.93 Archive stroke #1 of 8 @12:49:49.06 Archive.in :pressurize @12:49:49.14 Archive.seek :pressurized @12:50:01.60 2.9psi after pressurize @12:50:01.64 Archive.in :compress @12:50:01.74 Archive.seek :empty @12:50:17.51 15.4psi after compress @12:50:28.11 14.0psi after compress (stable) @12:50:28.14 Archive.in :gulp @12:50:28.25 Archive.seek :topVent @12:50:34.60 14.5psi after gulp @12:50:45.20 12.9psi after gulp (stable) @12:50:45.21 Archive stroke #2 of 8 @12:50:45.40 Archive.in :pressurize @12:50:45.48 Archive.seek :pressurized @12:50:57.96 12.8psi after pressurize @12:50:57.99 Archive.in :compress @12:50:58.09 Archive.seek :empty @12:51:12.69 Reached 24.4 of 24.3psi after pumping 3435uL (1.99 strokes) @12:51:12.73 Archive.in :default @12:51:12.85 Archive.hold @12:51:12.98 Handoff.select :waste @12:51:13.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:51:20.74 delay 1:00 @12:52:20.76 Handoff.select :blocked @12:52:20.96 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:52:43.61 Handoff.select :waste @12:52:43.77 0.4psi at evacEnd @12:52:43.80 Air.seek :fullVent @12:52:45.16 Archive.in :fast @12:52:45.26 Archive.seek :topVent @12:52:52.39 Seawater evacuated, Pressure above/below filter released. @12:52:52.52 Lysis1.in :slow @12:52:52.60 Lysis1.seek 1050uL @12:53:22.28 delay 5 seconds @12:53:27.32 Handoff.select :blocked @12:53:27.57 Lysis1.seek 450uL @12:54:26.78 delay 3 seconds @12:54:29.88 Air.in mix with 1.57127 parts Lysis1 @12:54:30.61 Air.seek :evac @12:54:30.65 Lysis1.seek 300uL @12:54:45.64 Lysis1.seek :empty @12:55:15.38 Lysis buffer delivered @11.0psi @12:55:15.40 Polling Can every 2:00 @12:55:15.98 PuckHeater.configure :default #ambient 21.9C @12:55:16.06 PuckHeater.seek 79.7C @12:56:04.74 PuckHeater.hold 2:00,79.7C @12:58:04.54 Polling Can every 2:00 @12:58:05.12 PuckHeater.configure :default #ambient 22.0C @12:58:05.24 PuckHeater.hold 30 seconds,78.7C @12:58:35.40 Polling Can every 2:00 @12:58:35.98 PuckHeater.configure :default #ambient 22.0C @12:58:36.06 PuckHeater.hold 30 seconds,78.2C @12:59:06.21 Polling Can every 2:00 @12:59:06.76 PuckHeater.configure :default #ambient 22.0C @12:59:06.85 PuckHeater.hold 10:00,77.7C @13:09:05.52 Lysis complete. Syringe pressure @3.1psi @13:09:05.55 Archive syringe pumping up to 5 strokes to reach 40psi @13:09:05.63 Archive stroke #1 of 5 @13:09:05.77 Archive.in :pressurize @13:09:05.92 Archive.seek :pressurized @13:09:18.40 3.6psi after pressurize @13:09:46.49 20.4psi after pressurize (stable) @13:09:46.52 Archive.in :compress @13:09:46.63 Archive.seek :empty @13:09:59.21 Reached 40.4 of 40.0psi after pumping 1662uL (0.96 strokes) @13:09:59.26 Archive.in :default @13:09:59.35 Archive.hold @13:09:59.45 delay 2 seconds @13:10:01.48 Archive.in :fast @13:10:01.56 Archive.seek :full @13:10:08.59 Lysis1.seek 138uL @13:10:22.32 delay 1 second @13:10:23.36 Archive.seek :full @13:10:23.44 Lysis1.seek :empty @13:10:37.21 Handoff.select :blocked @13:10:37.35 Initial air pressure @37.2psi @13:10:37.45 Lysis1.in :extract @13:10:37.57 Lysis1.seek 600uL,nil @13:10:41.84 Archive syringe pumping up to 6 strokes to reach 25psi @13:10:41.86 Archive stroke #1 of 6 @13:10:42.01 Archive.in :pressurize @13:10:42.10 Archive.seek :pressurized @13:10:43.15 Archive.in :default @13:10:43.22 Archive.hold @13:10:43.59 Initial 600uL lysate @12.6psi @13:10:43.61 Handoff.select :blocked @13:10:43.78 Initial air pressure @12.0psi @13:10:43.90 Lysis1.seek 825uL,nil @13:10:44.09 Archive syringe pumping up to 6 strokes to reach 17psi @13:10:44.11 Archive stroke #1 of 6 @13:10:44.30 Archive.in :pressurize @13:10:44.41 Archive.seek :pressurized @13:10:55.85 12.6psi after pressurize @13:11:14.85 Archive.in :default @13:11:14.93 Archive.hold @13:11:15.24 Recovered 825uL lysate @14.4psi @13:11:15.27 Air.in :halfSpeed @13:11:15.37 Air.seek :fullVent @13:11:17.94 Puck Top Vented, bottom @3.5psi @13:11:17.96 Handoff.select :waste @13:11:18.20 delay 5 seconds @13:11:23.28 Handoff open to waste, bottom @0.1psi @13:11:23.31 Air.in :default @13:11:23.44 Air.seek :evac @13:11:24.79 Lysis2.in :DAmix @13:11:24.95 Lysis1.in :DAmix @13:11:25.58 Lysis2.seek 700uL @13:11:25.68 Lysis1.seek 575uL @13:12:00.32 delay 5 seconds @13:12:05.36 Handoff.select SPR @13:12:05.53 SPR.command "start 2 14" @13:12:06.95 SPR stabilized @13:12:07.13 delay 10 seconds @13:12:17.15 SPR.command "start 2 0" @13:12:19.81 Waiting up to 11:00 for SPR to enter state 1 @13:14:17.46 SPR.command "hold_on" @13:14:19.37 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @13:14:20.07 Lysis2.seek 400uL @13:14:20.11 Lysis1.seek 275uL @13:15:01.67 delay 15 seconds @13:15:16.70 SPR.command "hold_off" @13:15:19.51 Waiting up to 1:00:00 for SPR to enter state 7 @13:31:34.13 SPRsummary:4.07RIU,269RIU,284RIU,5.17RIU,none,none,high after 2981s @13:45:51.05 SPR analysis completed @13:46:00.95 Ended log spr24jul3112h.spr.bz2 @13:46:00.97 SPR.command "stats_off" @13:46:01.28 Handoff.select :close @13:46:01.46 Gate.power :heat,:OFF @13:46:01.49 Gate.power :cartridge,:OFF @13:46:01.61 PinPort.in :unlocking @13:46:01.69 PinPort.seek :clear @13:46:02.19 Carousel.in :default @13:46:02.35 Carousel.home.seek 28 @13:46:02.74 Plunge.seek :locked @13:46:03.68 Archive.in :unlocking @13:46:04.30 Archive.seek :clear @13:46:04.49 Air.in :unlocking @13:46:04.63 Air.seek :clear @13:46:04.78 Lysis1.in :unlocking @13:46:04.92 Lysis1.seek :clear @13:46:05.08 Lysis2.in :unlocking @13:46:05.16 Lysis2.seek :clear @13:46:09.05 Gate.power :analytic1,:OFF @13:46:09.07 Gate.power :motor,:OFF @13:46:09.12 Gate.power :core,:OFF @13:46:09.15 Gate.power :main,:OFF @13:46:09.18 Safely stopped and ready to power off @13:56:10.71 -> PhytoSampler.goalVolume=75 75 @13:57:28.98 -> fire lysis @13:57:29.18 Gate.power :sampler,:OFF,limit to 3500mA # @13:57:29.26 Gate.power :skimmer,:OFF,limit to 3500mA @13:57:29.30 Gate.power :main,:ON,limit to 5000mA @13:57:29.34 Gate.power :analytic1,:ON,limit to 4000mA @13:57:29.44 Powering core bus @13:57:31.79 SPR Module 1, FW Rev. B, FPGA Rev. C @13:57:32.23 Plunge.configure :default @13:57:32.42 SamplePump.configure :default @13:57:32.53 Toroid.configure :default @13:57:32.70 Carousel.configure :default @13:57:32.81 Abs.configure :default @13:57:32.91 PhaseSeparator.configure :default @13:57:33.02 Archive.configure :default @13:57:33.13 Air.configure :default @13:57:33.21 Lysis1.configure :default @13:57:33.28 Lysis2.configure :default @13:57:33.38 PinPort.configure :default @13:57:33.45 All elf controllers running firmware version 4.92 @13:57:33.48 Gate.power :motor,:ON,limit to 5000mA @13:57:33.73 Plunge.home.jog 0 @13:57:34.57 PinPort.home.jog 0 @13:57:34.63 Lysis1.home.jog 0 @13:57:34.69 Archive.home.jog 0 @13:57:34.72 Carousel.home.jog 0 @13:57:34.78 Air.home.jog 0 @13:57:34.81 Toroid.home.jog 0 @13:57:34.87 Lysis2.home.jog 0 @13:57:35.68 Plunge.in :homing @13:57:36.01 Plunge.jog 1607 @13:57:36.28 Lysis1.in :homing @13:57:36.42 Air.in :homing @13:57:36.45 Toroid.in :homing @13:57:36.50 Lysis2.in :homing @13:57:36.54 Archive.in :homing @13:57:36.59 PinPort.in :homing @13:57:36.81 Air.jog 1371 @13:57:36.85 Lysis1.jog 1392 @13:57:37.06 Toroid.jog 619 @13:57:37.14 PinPort.jog -110 @13:57:37.22 Archive.jog 1395 @13:57:37.29 Lysis2.jog 1406 @13:57:37.68 PinPort.setRawPosition! 0 @13:57:37.70 Plunge.setRawPosition! 50 @13:57:37.85 PinPort.seek :home @13:57:37.87 Plunge.seek :home @13:57:38.12 PinPort.in :default @13:57:38.26 Air.setRawPosition! 50 @13:57:38.39 Air.seek :home @13:57:39.01 Lysis1.setRawPosition! 50 @13:57:39.06 Archive.setRawPosition! 50 @13:57:39.10 Lysis2.setRawPosition! 50 @13:57:39.15 Plunge.in :default @13:57:39.35 Lysis1.seek :home @13:57:39.39 Archive.seek :home @13:57:39.46 Lysis2.seek :home @13:57:39.89 Toroid.setRawPosition! 0 @13:57:39.93 Air.in :default @13:57:40.05 Toroid.seek :home @13:57:40.27 Lysis2.in :default @13:57:40.32 Lysis1.in :default @13:57:40.38 Archive.in :default @13:57:40.45 Toroid.in :default @13:57:40.92 Toroid.seek :clear @13:57:42.65 Carousel.in :default @13:57:42.80 Carousel.home.seek 28 @13:57:43.11 Selecting Cartridge 27 @13:57:43.43 Carousel.seek 27 @13:57:43.99 Lysis1.in :fast @13:57:44.08 Lysis1.seek :locked @13:57:46.73 Plunge.seek :locked @13:57:46.93 Air.in :fast @13:57:47.01 Air.seek :locked @13:57:48.01 Carousel.reconfigure :locked @13:57:48.20 Powering cartridge bus @13:57:50.86 Gate.setIR 1750,5000,0,6 @13:57:50.99 Gate.setIR 0,5000,0,6 @13:57:51.08 PuckHeater.configure :default #ambient 22.0C @13:57:51.18 Gate.power :heat,:ON,limit to 5000mA @13:57:51.89 Mounted lysis Cartridge board#Q05049 @13:57:51.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:57:52.00 GoodNews.email "PhytoSampler filtering up to 75 ml",Subject: "Sampling microSPR_phyto cartridge in slot 27" @13:57:52.42 Preparing to filter cartridge 27 @13:57:52.72 Air.in :default @13:57:52.86 Air.seek :sealed @13:57:53.01 Priming sample loop w/100ml, bypass w/10ml @13:57:53.04 SamplePump.setPosition! 0ml @13:57:53.14 Bypass.open @13:57:54.66 Intake.open @13:57:55.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:57:58.65 upsync @13:57:59.30 Vacuum tare is 52% of full scale at 22C (drifted 0%) @13:57:59.47 Abs.in :default @13:57:59.55 Abs.hold @13:57:59.90 SamplePump.in :start @13:58:00.00 SamplePump.seek 10ml,1:10 @13:58:00.59 SamplePump.configure :prime @13:58:12.57 Abs.stop @13:58:12.66 SamplePump.setPosition! 0ml @13:58:12.74 Exhaust.open @13:58:14.12 Bypass.close @13:58:15.66 Abs.in :default @13:58:15.75 Abs.hold @13:58:16.09 SamplePump.in :start @13:58:16.19 SamplePump.seek 100ml,7:10 @13:58:16.78 SamplePump.configure :prime @14:00:18.57 Abs.stop @14:00:18.68 Bypass.open @14:00:19.33 Intake.close @14:00:20.15 Toroid.in :hold @14:00:20.25 Toroid.seek :crack @14:00:21.81 Toroid.seek :cartridge @14:00:22.95 Toroid.in :default @14:00:23.04 Toroid.seek :clear @14:00:25.10 Pressurizing cartridge to 26psi above 15.0psia @14:00:25.12 SamplePump.setPosition! 0ml @14:00:25.22 Intake.open @14:00:25.91 Bypass.close @14:00:26.61 Exhaust.close @14:00:27.40 Abs.in :default @14:00:27.48 Abs.hold @14:00:27.73 SamplePump.in :charge @14:00:27.82 SamplePump.seek 2.3ml,42 seconds @14:00:43.26 Abs.stop @14:00:43.37 Intake.close @14:00:44.63 Pressurized cartridge with 1.073ml to 26.3psi @14:00:44.75 Isolated @34.3psia (19.3psi) @14:00:44.78 Verifying cartridge holds at least 0.65..0.85 of 34.3psia @14:00:44.84 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:00:49.45 Cartridge pressure fell 3% to 33.1psia @14:00:49.57 Sampling 75.0ml @14:00:49.59 SamplePump.setPosition! 0ml @14:00:49.69 Exhaust.open @14:00:50.33 Intake.open @14:00:51.27 Abs.in :default @14:00:51.36 Abs.hold @14:00:51.72 SamplePump.in :start @14:00:51.83 SamplePump.seek 75ml,6:45 @14:00:52.41 SamplePump.configure :sample @14:00:54.83 Cartridge 27 filtering 2/75ml at 0.56ml/s with 23.3psi vacuum @14:01:54.88 Cartridge 27 filtering 40/75ml at 0.64ml/s with 28.8psi vacuum @14:02:49.79 Abs.stop @14:02:49.98 Intake.close @14:02:50.03 Exhaust.close @14:02:50.30 Toroid.in :hold @14:02:50.41 Toroid.seek :bypass @14:02:52.08 Toroid.in :default @14:02:52.17 Toroid.seek :clear @14:02:53.81 Sampled 75.0ml @14:02:54.27 GoodNews.email "After filtering 75.0ml",Subject: "Processing microSPR_phyto cartridge in slot 27" @14:02:55.93 SPR.command "read_sequences" @14:02:56.10 SPR.command "stats_on" @14:02:56.32 SPR.command "start 0 1" @14:02:57.11 SPR.start /var/log/esp/spr24jul3114h.spr.bz2 @14:02:59.23 upsync @14:02:59.47 Handoff.select :waste @14:02:59.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:03:06.28 Syringe tare is 51% of full scale at 22C (drifted 0%) @14:03:06.32 PinPort.in :locking @14:03:06.45 PinPort.seek :locked @14:03:07.00 Archive.in :fast @14:03:07.06 Lysis2.in :fast @14:03:07.17 Lysis1.seek 1350uL @14:03:07.33 Archive.seek :full @14:03:07.42 Lysis2.seek 950uL @14:03:12.28 Plunge.seek :reagent @14:03:13.25 Evacuating puck with filter bubble point of 24psi @14:03:13.29 PinPort.seek :locked @14:03:13.42 Handoff.select :waste @14:03:13.46 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:03:20.07 Air syringe pumping up to 1 strokes to reach 8psi @14:03:20.09 Air stroke #1 of 1 @14:03:20.23 Air.in :pressurize @14:03:20.34 Air.seek :pressurized @14:03:26.03 1.6psi after pressurize @14:03:26.06 Air.in :compress @14:03:26.17 Air.seek :evac @14:03:29.21 2.1psi after compress @14:03:42.81 1.5psi after compress (stable) @14:03:42.83 Air.in :default @14:03:42.94 Air.hold @14:03:43.23 at 1.2psi Opened to waste @14:03:43.25 Handoff.select :blocked @14:03:43.44 Archive syringe pumping up to 8 strokes to reach 24psi @14:03:43.46 Archive stroke #1 of 8 @14:03:43.58 Archive.in :pressurize @14:03:43.68 Archive.seek :pressurized @14:03:56.16 2.3psi after pressurize @14:04:21.79 7.7psi after pressurize (stable) @14:04:21.83 Archive.in :compress @14:04:21.92 Archive.seek :empty @14:04:37.68 12.1psi after compress @14:04:48.77 14.8psi after compress (stable) @14:04:48.80 Archive.in :gulp @14:04:48.91 Archive.seek :topVent @14:04:55.25 16.0psi after gulp @14:05:00.84 15.1psi after gulp (stable) @14:05:00.86 Archive stroke #2 of 8 @14:05:01.00 Archive.in :pressurize @14:05:01.08 Archive.seek :pressurized @14:05:13.54 15.5psi after pressurize @14:05:19.13 14.7psi after pressurize (stable) @14:05:19.16 Archive.in :compress @14:05:19.27 Archive.seek :empty @14:05:30.85 Reached 23.8 of 24.0psi after pumping 3369uL (1.95 strokes) @14:05:30.88 Archive.in :default @14:05:30.99 Archive.hold @14:05:31.11 Handoff.select :waste @14:05:31.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:05:42.89 delay 1:00 @14:06:42.91 Handoff.select :blocked @14:06:43.08 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:07:05.67 Handoff.select :waste @14:07:05.83 0.7psi at evacEnd @14:07:05.85 Handoff.select :blocked @14:07:06.01 Archive.in :fast @14:07:06.09 Archive.seek :topVent @14:07:12.97 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @14:07:19.61 Stable @0.3psi after Handoff blocked @14:07:19.65 Archive syringe pumping up to 8 strokes to reach 24psi @14:07:19.67 Archive stroke #1 of 8 @14:07:19.81 Archive.in :pressurize @14:07:19.90 Archive.seek :pressurized @14:07:32.39 1.9psi after pressurize @14:07:32.42 Archive.in :compress @14:07:32.53 Archive.seek :empty @14:07:48.28 14.6psi after compress @14:07:58.88 13.3psi after compress (stable) @14:07:58.91 Archive.in :gulp @14:07:59.00 Archive.seek :topVent @14:08:05.33 14.3psi after gulp @14:08:10.92 13.7psi after gulp (stable) @14:08:10.94 Archive stroke #2 of 8 @14:08:11.08 Archive.in :pressurize @14:08:11.16 Archive.seek :pressurized @14:08:23.63 14.2psi after pressurize @14:08:23.66 Archive.in :compress @14:08:23.76 Archive.seek :empty @14:08:36.39 Reached 24.2 of 24.3psi after pumping 3392uL (1.96 strokes) @14:08:36.42 Archive.in :default @14:08:36.53 Archive.hold @14:08:36.63 Handoff.select :waste @14:08:36.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:08:43.89 delay 1:00 @14:09:43.91 Handoff.select :blocked @14:09:44.08 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:10:06.67 Handoff.select :waste @14:10:06.83 0.5psi at evacEnd @14:10:06.86 Air.seek :fullVent @14:10:08.22 Archive.in :fast @14:10:08.31 Archive.seek :topVent @14:10:15.29 Seawater evacuated, Pressure above/below filter released. @14:10:15.41 Lysis1.in :slow @14:10:15.54 Lysis1.seek 1050uL @14:10:45.20 delay 5 seconds @14:10:50.22 Handoff.select :blocked @14:10:50.48 Lysis1.seek 450uL @14:11:49.67 delay 3 seconds @14:11:52.76 Air.in mix with 1.57127 parts Lysis1 @14:11:53.58 Air.seek :evac @14:11:53.68 Lysis1.seek 300uL @14:12:08.63 Lysis1.seek :empty @14:12:38.44 Lysis buffer delivered @10.5psi @14:12:38.46 Polling Can every 2:00 @14:12:39.08 PuckHeater.configure :default #ambient 22.1C @14:12:39.16 PuckHeater.seek 79.7C @14:13:29.07 PuckHeater.hold 2:00,79.7C @14:15:28.59 Polling Can every 2:00 @14:15:29.14 PuckHeater.configure :default #ambient 22.1C @14:15:29.22 PuckHeater.hold 30 seconds,78.7C @14:15:59.27 Polling Can every 2:00 @14:15:59.83 PuckHeater.hold 30 seconds,78.2C @14:16:30.39 Polling Can every 2:00 @14:16:31.03 PuckHeater.configure :default #ambient 22.1C @14:16:31.12 PuckHeater.hold 10:00,77.6C @14:26:28.09 Lysis complete. Syringe pressure @13.7psi @14:26:28.12 Archive syringe pumping up to 5 strokes to reach 40psi @14:26:28.15 Archive stroke #1 of 5 @14:26:28.28 Archive.in :pressurize @14:26:28.37 Archive.seek :pressurized @14:26:40.81 14.2psi after pressurize @14:26:46.43 13.5psi after pressurize (stable) @14:26:46.46 Archive.in :compress @14:26:46.55 Archive.seek :empty @14:26:57.86 Reached 40.6 of 40.0psi after pumping 1635uL (0.94 strokes) @14:26:57.88 Archive.in :default @14:26:57.99 Archive.hold @14:26:58.61 delay 2 seconds @14:27:00.73 Archive.in :fast @14:27:00.81 Archive.seek :full @14:27:07.74 Lysis1.seek 138uL @14:27:21.46 delay 1 second @14:27:22.50 Archive.seek :full @14:27:22.54 Lysis1.seek :empty @14:27:36.40 Handoff.select :blocked @14:27:36.54 Initial air pressure @41.3psi @14:27:36.65 Lysis1.in :extract @14:27:36.79 Lysis1.seek 600uL,nil @14:27:41.68 Archive syringe pumping up to 6 strokes to reach 25psi @14:27:41.70 Archive stroke #1 of 6 @14:27:41.84 Archive.in :pressurize @14:27:41.95 Archive.seek :pressurized @14:27:42.41 Archive.in :default @14:27:42.49 Archive.hold @14:27:42.88 Initial 600uL lysate @14.3psi @14:27:42.91 Handoff.select :blocked @14:27:43.57 Initial air pressure @14.0psi @14:27:43.77 Lysis1.seek 825uL,nil @14:27:44.00 Archive syringe pumping up to 6 strokes to reach 17psi @14:27:44.03 Archive stroke #1 of 6 @14:27:44.22 Archive.in :pressurize @14:27:44.42 Archive.seek :pressurized @14:27:56.44 14.3psi after pressurize @14:28:02.09 13.5psi after pressurize (stable) @14:28:02.12 Archive.in :compress @14:28:02.22 Archive.seek :empty @14:28:05.88 Reached 17.1 of 17.0psi after pumping 1471uL (0.85 strokes) @14:28:05.91 Archive.in :default @14:28:06.01 Archive.hold @14:28:08.05 Archive syringe pumping up to 6 strokes to reach 17psi @14:28:08.09 Archive stroke #1 of 6 @14:28:08.25 Archive.in :compress @14:28:08.33 Archive.seek :empty @14:28:08.93 Archive.in :default @14:28:09.01 Archive.hold @14:28:09.35 Recovered 825uL lysate @16.0psi @14:28:09.38 Air.in :halfSpeed @14:28:09.48 Air.seek :fullVent @14:28:11.52 Puck Top Vented, bottom @18.2psi @14:28:11.53 Handoff.select :waste @14:28:11.71 delay 5 seconds @14:28:16.79 Handoff open to waste, bottom @0.2psi @14:28:16.81 Air.in :default @14:28:16.92 Air.seek :evac @14:28:18.26 Lysis2.in :DAmix @14:28:18.48 Lysis1.in :DAmix @14:28:19.26 Lysis2.seek 700uL @14:28:19.39 Lysis1.seek 575uL @14:28:53.96 delay 5 seconds @14:28:58.98 Handoff.select SPR @14:28:59.15 SPR.command "start 2 14" @14:29:00.52 SPR stabilized @14:29:00.72 delay 10 seconds @14:29:10.74 SPR.command "start 2 0" @14:29:13.89 Waiting up to 11:00 for SPR to enter state 1 @14:31:11.56 SPR.command "hold_on" @14:31:13.47 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:31:14.17 Lysis2.seek 400uL @14:31:14.21 Lysis1.seek 275uL @14:31:55.77 delay 15 seconds @14:32:10.79 SPR.command "hold_off" @14:32:14.12 Waiting up to 1:00:00 for SPR to enter state 7 @14:48:29.17 SPRsummary:-394RIU,65.8RIU,827RIU,3.1RIU,none,none,none after 2886s @14:55:31.50 -> PhytoSampler.xray @14:55:46.58 -> PytoSampler.goalVolume=50 @14:55:46.61 NameError in bill -- uninitialized constant PytoSampler @14:56:00.71 -> PhytoSampler.goalVolume=50 50 @14:56:05.53 -> fire lysis @14:56:05.76 Cmd::Error in detached from bill#2 -- Cannot load cartridge while in PROCESSING state # @14:56:05.84 BadNews.email "Cannot load cartridge while in PROCESSING state",Subject: "Cmd::Error in detached from bill#2" @14:56:11.85 upsync @15:02:46.07 SPR analysis completed @15:02:54.82 Ended log spr24jul3114h.spr.bz2 @15:02:54.85 SPR.command "stats_off" @15:02:55.27 Handoff.select :close @15:02:55.44 Gate.power :heat,:OFF @15:02:55.47 Gate.power :cartridge,:OFF @15:02:55.59 PinPort.in :unlocking @15:02:55.67 PinPort.seek :clear @15:02:56.18 Carousel.in :default @15:02:56.84 Carousel.home.seek 27 @15:02:57.23 Plunge.seek :locked @15:02:58.16 Archive.in :unlocking @15:02:58.32 Archive.seek :clear @15:02:58.44 Air.in :unlocking @15:02:58.64 Air.seek :clear @15:02:58.78 Lysis1.in :unlocking @15:02:58.94 Lysis1.seek :clear @15:02:59.08 Lysis2.in :unlocking @15:02:59.17 Lysis2.seek :clear @15:03:03.03 Gate.power :analytic1,:OFF @15:03:03.06 Gate.power :motor,:OFF @15:03:03.10 Gate.power :core,:OFF @15:03:03.13 Gate.power :main,:OFF @15:03:03.16 Safely stopped and ready to power off @15:03:30.93 -> PhytoSampler.goalVolume 50 @15:03:43.00 -> fire lysis # @15:03:43.22 Gate.power :sampler,:OFF,limit to 3500mA @15:03:43.28 Gate.power :skimmer,:OFF,limit to 3500mA @15:03:43.32 Gate.power :main,:ON,limit to 5000mA @15:03:43.36 Gate.power :analytic1,:ON,limit to 4000mA @15:03:43.47 Powering core bus @15:03:45.82 SPR Module 1, FW Rev. B, FPGA Rev. C @15:03:46.26 Plunge.configure :default @15:03:46.40 SamplePump.configure :default @15:03:46.50 Toroid.configure :default @15:03:46.59 Carousel.configure :default @15:03:46.70 Abs.configure :default @15:03:46.81 PhaseSeparator.configure :default @15:03:46.91 Archive.configure :default @15:03:47.03 Air.configure :default @15:03:47.09 Lysis1.configure :default @15:03:47.17 Lysis2.configure :default @15:03:47.28 PinPort.configure :default @15:03:47.35 All elf controllers running firmware version 4.92 @15:03:47.38 Gate.power :motor,:ON,limit to 5000mA @15:03:47.70 Plunge.home.jog 0 @15:03:48.40 Lysis2.home.jog 0 @15:03:48.48 Lysis1.home.jog 0 @15:03:48.53 Archive.home.jog 0 @15:03:48.61 Carousel.home.jog 0 @15:03:48.68 PinPort.home.jog 0 @15:03:48.72 Air.home.jog 0 @15:03:48.78 Toroid.home.jog 0 @15:03:49.72 Plunge.in :homing @15:03:49.99 Plunge.jog 1608 @15:03:50.07 Lysis2.in :homing @15:03:50.13 Air.in :homing @15:03:50.16 Toroid.in :homing @15:03:50.22 PinPort.in :homing @15:03:50.27 Lysis1.in :homing @15:03:50.30 Archive.in :homing @15:03:51.32 PinPort.jog -109 @15:03:51.38 Lysis2.jog 1406 @15:03:51.42 Lysis1.jog 1392 @15:03:51.47 Air.jog 1370 @15:03:51.54 Archive.jog 1397 @15:03:51.58 Toroid.jog 615 @15:03:52.09 PinPort.setRawPosition! 0 @15:03:52.11 Plunge.setRawPosition! 50 @15:03:52.28 PinPort.seek :home @15:03:52.30 Plunge.seek :home @15:03:52.60 PinPort.in :default @15:03:52.71 Plunge.in :default @15:03:52.86 Lysis1.setRawPosition! 50 @15:03:52.96 Lysis1.seek :home @15:03:53.13 Air.setRawPosition! 50 @15:03:53.18 Archive.setRawPosition! 50 @15:03:53.22 Lysis2.setRawPosition! 50 @15:03:53.39 Archive.seek :home @15:03:53.45 Air.seek :home @15:03:53.53 Lysis2.seek :home @15:03:53.81 Archive.in :default @15:03:53.84 Lysis1.in :default @15:03:54.06 Air.in :default @15:03:54.09 Toroid.setRawPosition! 0 @15:03:54.13 Lysis2.in :default @15:03:54.33 Toroid.seek :home @15:03:54.49 Toroid.in :default @15:03:54.63 Toroid.seek :clear @15:03:56.39 Carousel.in :default @15:03:56.54 Carousel.home.seek 27 @15:03:56.83 Selecting Cartridge 26 @15:03:57.16 Carousel.seek 26 @15:03:57.73 Lysis1.in :fast @15:03:57.81 Lysis1.seek :locked @15:04:00.48 Plunge.seek :locked @15:04:00.68 Air.in :fast @15:04:00.77 Air.seek :locked @15:04:01.76 Carousel.reconfigure :locked @15:04:01.95 Powering cartridge bus @15:04:04.62 Gate.setIR 1750,5000,0,6 @15:04:04.74 Gate.setIR 0,5000,0,6 @15:04:04.83 PuckHeater.configure :default #ambient 22.1C @15:04:04.93 Gate.power :heat,:ON,limit to 5000mA @15:04:05.64 Mounted lysis Cartridge board#Q05087 @15:04:05.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:04:05.76 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 26" @15:04:06.71 Preparing to filter cartridge 26 @15:04:07.06 Air.in :default @15:04:07.25 Air.seek :sealed @15:04:07.31 Priming sample loop w/100ml, bypass w/10ml @15:04:07.41 SamplePump.setPosition! 0ml @15:04:07.50 Bypass.open @15:04:09.01 Intake.open @15:04:10.01 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:04:12.66 Vacuum tare is 53% of full scale at 22C (drifted 1%) @15:04:12.85 Abs.in :default @15:04:12.93 Abs.hold @15:04:13.21 SamplePump.in :start @15:04:13.31 SamplePump.seek 10ml,1:10 @15:04:13.91 SamplePump.configure :prime @15:04:25.89 Abs.stop @15:04:25.98 SamplePump.setPosition! 0ml @15:04:26.06 Exhaust.open @15:04:27.49 Bypass.close @15:04:28.50 upsync @15:04:29.04 Abs.in :default @15:04:29.12 Abs.hold @15:04:29.47 SamplePump.in :start @15:04:29.56 SamplePump.seek 100ml,7:10 @15:04:30.16 SamplePump.configure :prime @15:06:31.94 Abs.stop @15:06:32.07 Bypass.open @15:06:32.78 Intake.close @15:06:33.59 Toroid.in :hold @15:06:33.68 Toroid.seek :crack @15:06:35.26 Toroid.seek :cartridge @15:06:36.43 Toroid.in :default @15:06:36.98 Toroid.seek :clear @15:06:39.13 Pressurizing cartridge to 26psi above 15.0psia @15:06:39.15 SamplePump.setPosition! 0ml @15:06:39.26 Intake.open @15:06:39.95 Bypass.close @15:06:40.66 Exhaust.close @15:06:41.47 Abs.in :default @15:06:41.56 Abs.hold @15:06:41.81 SamplePump.in :charge @15:06:41.90 SamplePump.seek 2.3ml,42 seconds @15:06:55.54 Abs.stop @15:06:55.64 Intake.close @15:06:56.42 Pressurized cartridge with 0.836ml to 27.6psi @15:06:56.43 Isolated @37.3psia (22.3psi) @15:06:56.46 Verifying cartridge holds at least 0.65..0.85 of 37.3psia @15:06:56.55 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:07:01.22 Sampling 50.0ml @15:07:01.24 SamplePump.setPosition! 0ml @15:07:01.34 Exhaust.open @15:07:01.99 Intake.open @15:07:02.86 Abs.in :default @15:07:02.94 Abs.hold @15:07:03.29 SamplePump.in :start @15:07:03.39 SamplePump.seek 50ml,4:40 @15:07:03.98 SamplePump.configure :sample @15:07:06.42 Cartridge 26 filtering 2/50ml at 0.67ml/s with 23.7psi vacuum @15:08:06.47 Cartridge 26 filtering 42/50ml at 0.67ml/s with 19.9psi vacuum @15:08:18.30 Abs.stop @15:08:18.51 Intake.close @15:08:18.58 Exhaust.close @15:08:18.84 Toroid.in :hold @15:08:18.92 Toroid.seek :bypass @15:08:20.59 Toroid.in :default @15:08:20.69 Toroid.seek :clear @15:08:22.34 Sampled 50.0ml @15:08:23.37 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 26" @15:08:25.05 SPR.command "read_sequences" @15:08:25.67 SPR.command "stats_on" @15:08:25.89 SPR.command "start 0 1" @15:08:26.60 SPR.start /var/log/esp/spr24jul3115h.spr.bz2 @15:08:29.05 Handoff.select :waste @15:08:29.23 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:08:29.32 upsync @15:08:35.87 Syringe tare is 51% of full scale at 22C (drifted 0%) @15:08:35.91 PinPort.in :locking @15:08:36.01 PinPort.seek :locked @15:08:36.55 Archive.in :fast @15:08:36.71 Lysis2.in :fast @15:08:36.76 Lysis1.seek 1350uL @15:08:36.81 Archive.seek :full @15:08:37.02 Lysis2.seek 950uL @15:08:41.83 Plunge.seek :reagent @15:08:42.78 Evacuating puck with filter bubble point of 24psi @15:08:42.82 PinPort.seek :locked @15:08:42.96 Handoff.select :waste @15:08:43.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:08:49.61 Air syringe pumping up to 1 strokes to reach 8psi @15:08:49.62 Air stroke #1 of 1 @15:08:49.76 Air.in :pressurize @15:08:49.87 Air.seek :pressurized @15:08:55.59 1.1psi after pressurize @15:08:55.62 Air.in :compress @15:08:55.72 Air.seek :evac @15:08:58.76 2.2psi after compress @15:09:09.35 1.0psi after compress (stable) @15:09:09.37 Air.in :default @15:09:09.53 Air.hold @15:09:09.88 at 0.7psi Opened to waste @15:09:09.91 Handoff.select :blocked @15:09:10.09 Archive syringe pumping up to 8 strokes to reach 24psi @15:09:10.10 Archive stroke #1 of 8 @15:09:10.24 Archive.in :pressurize @15:09:10.32 Archive.seek :pressurized @15:09:22.82 2.1psi after pressurize @15:09:34.45 3.2psi after pressurize (stable) @15:09:34.48 Archive.in :compress @15:09:34.58 Archive.seek :empty @15:09:50.34 13.8psi after compress @15:10:00.99 13.7psi after compress (stable) @15:10:01.02 Archive.in :gulp @15:10:01.13 Archive.seek :topVent @15:10:07.50 14.6psi after gulp @15:10:13.12 14.0psi after gulp (stable) @15:10:13.16 Archive stroke #2 of 8 @15:10:13.30 Archive.in :pressurize @15:10:13.38 Archive.seek :pressurized @15:10:25.84 14.5psi after pressurize @15:10:31.45 13.7psi after pressurize (stable) @15:10:31.48 Archive.in :compress @15:10:31.58 Archive.seek :empty @15:10:45.12 Reached 24.3 of 24.0psi after pumping 3412uL (1.97 strokes) @15:10:45.14 Archive.in :default @15:10:45.27 Archive.hold @15:10:45.91 Handoff.select :waste @15:10:46.08 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:10:58.68 delay 1:00 @15:11:58.70 Handoff.select :blocked @15:11:58.87 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @15:12:21.97 Handoff.select :waste @15:12:22.13 0.6psi at evacEnd @15:12:22.14 Handoff.select :blocked @15:12:22.31 Archive.in :fast @15:12:22.42 Archive.seek :topVent @15:12:29.99 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @15:12:36.58 Stable @0.5psi after Handoff blocked @15:12:36.61 Archive syringe pumping up to 8 strokes to reach 24psi @15:12:36.64 Archive stroke #1 of 8 @15:12:36.77 Archive.in :pressurize @15:12:36.91 Archive.seek :pressurized @15:12:49.36 1.2psi after pressurize @15:12:49.39 Archive.in :compress @15:12:49.50 Archive.seek :empty @15:13:05.29 13.9psi after compress @15:13:15.89 13.5psi after compress (stable) @15:13:15.92 Archive.in :gulp @15:13:16.08 Archive.seek :topVent @15:13:22.45 14.3psi after gulp @15:13:28.05 13.7psi after gulp (stable) @15:13:28.06 Archive stroke #2 of 8 @15:13:28.21 Archive.in :pressurize @15:13:28.29 Archive.seek :pressurized @15:13:40.76 14.2psi after pressurize @15:13:40.79 Archive.in :compress @15:13:40.90 Archive.seek :empty @15:13:55.36 Reached 24.5 of 24.5psi after pumping 3431uL (1.98 strokes) @15:13:55.38 Archive.in :default @15:13:55.49 Archive.hold @15:13:55.61 Handoff.select :waste @15:13:55.75 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:14:07.85 delay 1:00 @15:15:07.87 Handoff.select :blocked @15:15:08.04 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @15:15:30.64 Handoff.select :waste @15:15:30.79 0.7psi at evacEnd @15:15:30.84 Air.seek :fullVent @15:15:32.22 Archive.in :fast @15:15:32.30 Archive.seek :topVent @15:15:39.40 Seawater evacuated, Pressure above/below filter released. @15:15:39.53 Lysis1.in :slow @15:15:39.62 Lysis1.seek 1050uL @15:16:09.28 delay 5 seconds @15:16:14.30 Handoff.select :blocked @15:16:14.62 Lysis1.seek 450uL @15:17:13.80 delay 3 seconds @15:17:16.90 Air.in mix with 1.57127 parts Lysis1 @15:17:17.65 Air.seek :evac @15:17:17.69 Lysis1.seek 300uL @15:17:32.73 Lysis1.seek :empty @15:18:02.48 Lysis buffer delivered @9.0psi @15:18:02.50 Polling Can every 2:00 @15:18:03.08 PuckHeater.configure :default #ambient 21.9C @15:18:03.21 PuckHeater.seek 79.7C @15:18:53.21 PuckHeater.hold 2:00,79.7C @15:20:53.44 Polling Can every 2:00 @15:20:53.99 PuckHeater.configure :default #ambient 22.0C @15:20:54.09 PuckHeater.hold 30 seconds,78.7C @15:21:24.34 Polling Can every 2:00 @15:21:24.92 PuckHeater.configure :default #ambient 22.0C @15:21:25.00 PuckHeater.hold 30 seconds,78.2C @15:21:55.41 Polling Can every 2:00 @15:21:56.01 PuckHeater.configure :default #ambient 21.9C @15:21:56.10 PuckHeater.hold 10:00,77.7C @15:31:56.65 Lysis complete. Syringe pressure @11.9psi @15:31:56.68 Archive syringe pumping up to 5 strokes to reach 40psi @15:31:56.72 Archive stroke #1 of 5 @15:31:56.87 Archive.in :pressurize @15:31:56.95 Archive.seek :pressurized @15:32:09.40 12.5psi after pressurize @15:32:26.00 13.8psi after pressurize (stable) @15:32:26.03 Archive.in :compress @15:32:26.13 Archive.seek :empty @15:32:40.98 Reached 40.6 of 40.0psi after pumping 1709uL (0.99 strokes) @15:32:41.00 Archive.in :default @15:32:41.11 Archive.hold @15:32:41.22 delay 2 seconds @15:32:43.25 Archive.in :fast @15:32:43.33 Archive.seek :full @15:32:50.52 Lysis1.seek 138uL @15:33:04.25 delay 1 second @15:33:05.28 Archive.seek :full @15:33:05.38 Lysis1.seek :empty @15:33:19.16 Handoff.select :blocked @15:33:19.30 Initial air pressure @41.7psi @15:33:19.40 Lysis1.in :extract @15:33:20.14 Lysis1.seek 600uL,nil @15:33:25.05 Archive syringe pumping up to 6 strokes to reach 25psi @15:33:25.07 Archive stroke #1 of 6 @15:33:25.27 Archive.in :pressurize @15:33:25.40 Archive.seek :pressurized @15:33:25.67 Archive.in :default @15:33:25.74 Archive.hold @15:33:26.13 Initial 600uL lysate @15.5psi @15:33:26.15 Handoff.select :blocked @15:33:26.32 Initial air pressure @15.4psi @15:33:26.47 Lysis1.seek 825uL,nil @15:33:26.68 Archive syringe pumping up to 6 strokes to reach 17psi @15:33:26.70 Archive stroke #1 of 6 @15:33:26.91 Archive.in :pressurize @15:33:26.99 Archive.seek :pressurized @15:33:27.40 Reached 16.9 of 17.0psi after pumping 39uL (0.02 strokes) @15:33:27.43 Archive.in :default @15:33:27.53 Archive.hold @15:33:27.82 Archive syringe pumping up to 6 strokes to reach 17psi @15:33:27.86 Archive stroke #1 of 6 @15:33:28.05 Archive.in :pressurize @15:33:28.16 Archive.seek :pressurized @15:33:40.36 14.2psi after pressurize @15:33:45.98 13.5psi after pressurize (stable) @15:33:46.01 Archive.in :compress @15:33:46.15 Archive.seek :empty @15:33:54.16 Archive.in :default @15:33:54.27 Archive.hold @15:33:54.61 Recovered 825uL lysate @16.6psi @15:33:54.66 Air.in :halfSpeed @15:33:54.78 Air.seek :fullVent @15:33:56.83 Puck Top Vented, bottom @17.6psi @15:33:56.85 Handoff.select :waste @15:33:57.03 delay 5 seconds @15:34:02.11 Handoff open to waste, bottom @0.8psi @15:34:02.14 Air.in :default @15:34:02.26 Air.seek :evac @15:34:03.61 Lysis2.in :DAmix @15:34:03.76 Lysis1.in :DAmix @15:34:04.52 Lysis2.seek 700uL @15:34:04.62 Lysis1.seek 575uL @15:34:39.24 delay 5 seconds @15:34:44.27 Handoff.select SPR @15:34:44.45 SPR.command "start 2 14" @15:34:45.23 SPR stabilized @15:34:45.43 delay 10 seconds @15:34:55.45 SPR.command "start 2 0" @15:34:58.60 Waiting up to 11:00 for SPR to enter state 1 @15:36:56.26 SPR.command "hold_on" @15:36:58.17 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:36:58.92 Lysis2.seek 400uL @15:36:58.97 Lysis1.seek 275uL @15:37:40.51 delay 15 seconds @15:37:55.53 SPR.command "hold_off" @15:37:58.31 Waiting up to 1:00:00 for SPR to enter state 7 @15:54:12.93 SPRsummary:2.64RIU,76.9RIU,90RIU,3.08RIU,229ng/L,161ng/L,high after 2901s @16:08:29.82 SPR analysis completed @16:08:38.87 Ended log spr24jul3115h.spr.bz2 @16:08:38.90 SPR.command "stats_off" @16:08:39.92 Handoff.select :close @16:08:40.10 Gate.power :heat,:OFF @16:08:40.12 Gate.power :cartridge,:OFF @16:08:40.24 PinPort.in :unlocking @16:08:40.31 PinPort.seek :clear @16:08:40.86 Carousel.in :default @16:08:41.00 Carousel.home.seek 26 @16:08:41.36 Plunge.seek :locked @16:08:42.29 Archive.in :unlocking @16:08:42.47 Archive.seek :clear @16:08:42.61 Air.in :unlocking @16:08:42.77 Air.seek :clear @16:08:42.90 Lysis1.in :unlocking @16:08:43.57 Lysis1.seek :clear @16:08:43.71 Lysis2.in :unlocking @16:08:43.81 Lysis2.seek :clear @16:08:47.74 Gate.power :analytic1,:OFF @16:08:47.76 Gate.power :motor,:OFF @16:08:47.81 Gate.power :core,:OFF @16:08:47.84 Gate.power :main,:OFF @16:08:47.87 Safely stopped and ready to power off @16:08:59.46 -> Handoff Handoff Valve::Manifold selects blocked @16:09:07.22 -> Handoff.waste @16:09:07.24 Handoff.select :waste @16:09:07.28 BusOff in bill -- Handoff valve is not powered! @16:09:16.05 -> ESP.ready! @16:09:16.11 Gate.power :sampler,:OFF,limit to 3500mA @16:09:16.14 Gate.power :skimmer,:OFF,limit to 3500mA @16:09:16.18 Gate.power :main,:ON,limit to 5000mA @16:09:16.22 Gate.power :analytic1,:ON,limit to 4000mA @16:09:16.33 Powering core bus @16:09:18.68 SPR Module 1, FW Rev. B, FPGA Rev. C @16:09:19.14 Plunge.configure :default @16:09:19.25 SamplePump.configure :default @16:09:19.35 Toroid.configure :default @16:09:19.45 Carousel.configure :default @16:09:19.55 Abs.configure :default @16:09:19.67 PhaseSeparator.configure :default @16:09:19.77 Archive.configure :default @16:09:19.88 Air.configure :default @16:09:19.96 Lysis1.configure :default @16:09:20.03 Lysis2.configure :default @16:09:20.13 PinPort.configure :default @16:09:20.21 All elf controllers running firmware version 4.92 @16:09:20.25 Gate.power :motor,:ON,limit to 5000mA @16:09:20.58 Plunge.home.jog 0 @16:09:21.27 Carousel.home.jog 0 @16:09:21.37 Lysis2.home.jog 0 @16:09:21.41 Air.home.jog 0 @16:09:21.47 Toroid.home.jog 0 @16:09:21.52 PinPort.home.jog 0 @16:09:21.56 Lysis1.home.jog 0 @16:09:21.61 Archive.home.jog 0 @16:09:22.54 Plunge.in :homing @16:09:22.98 Plunge.jog 1603 @16:09:23.06 PinPort.in :homing @16:09:23.11 Lysis1.in :homing @16:09:23.15 Air.in :homing @16:09:23.18 Toroid.in :homing @16:09:23.23 Lysis2.in :homing @16:09:23.28 Archive.in :homing @16:09:23.63 Lysis1.jog 1391 @16:09:23.67 PinPort.jog -110 @16:09:23.76 Air.jog 1368 @16:09:23.82 Lysis2.jog 1406 @16:09:23.86 Toroid.jog 621 @16:09:23.98 Archive.jog 1397 @16:09:24.39 PinPort.setRawPosition! 0 @16:09:24.47 PinPort.seek :home @16:09:24.61 PinPort.in :default @16:09:24.63 Plunge.setRawPosition! 50 @16:09:24.78 Plunge.seek :home @16:09:25.48 Lysis2.setRawPosition! 50 @16:09:25.57 Lysis1.setRawPosition! 50 @16:09:25.60 Air.setRawPosition! 50 @16:09:25.66 Archive.setRawPosition! 50 @16:09:25.89 Lysis2.seek :home @16:09:25.95 Lysis1.seek :home @16:09:26.00 Air.seek :home @16:09:26.04 Archive.seek :home @16:09:26.60 Lysis1.in :default @16:09:26.65 Air.in :default @16:09:26.68 Plunge.in :default @16:09:26.71 Lysis2.in :default @16:09:26.75 Toroid.setRawPosition! 0 @16:09:27.04 Toroid.seek :home @16:09:27.19 Toroid.in :default @16:09:27.22 Archive.in :default @16:09:27.62 Toroid.seek :clear @16:09:29.31 Carousel.in :default @16:09:29.46 Carousel.home.seek 26 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 26 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:09:36.33 -> Handoff.waste @16:09:36.37 Handoff.select :waste Handoff Valve::Manifold selects waste @16:11:30.49 -> Handoff.AM @16:11:30.51 Handoff.select :AM Handoff Valve::Manifold selects Analytical Module @16:13:16.96 -> Cmd.stop @16:13:17.10 Handoff.select :close @16:13:17.35 Carousel.in :default @16:13:17.50 Carousel.home.seek 26 @16:13:17.87 Lysis1.in :fast @16:13:17.95 Lysis1.seek :locked @16:13:20.50 Plunge.seek :locked @16:13:21.87 Lysis1.in :unlocking @16:13:22.05 Lysis1.seek :clear @16:13:23.57 Gate.power :analytic1,:OFF @16:13:23.59 Gate.power :motor,:OFF @16:13:23.64 Gate.power :core,:OFF @16:13:23.67 Gate.power :main,:OFF @16:13:23.71 Safely stopped and ready to power off :STOPPED @16:13:30.29 -> exit @16:13:30.32 Session Ended @03:23:39.84PDT01-Aug-24 Session Begun @03:23:43.19 -> slot {26 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24jul3115h.spr.bz2}, 27 => { Type: :microSPR_phyto, State: :processed, filtered: 75.0, lysed: 825, spr: spr24jul3114h.spr.bz2}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 825, spr: spr24jul3112h.spr.bz2}} @03:24:11.67 -> slot [60..1] =>nil {60..1 => nil} @03:24:23.51 -> ls :ls @03:24:29.60 -> exit @03:24:29.63 Session Ended @04:56:58.78 Session Begun @04:57:06.00 -> ESP.ready! @04:57:06.06 Gate.power :sampler,:OFF,limit to 3500mA @04:57:06.09 Gate.power :skimmer,:OFF,limit to 3500mA @04:57:06.14 Gate.power :main,:ON,limit to 5000mA @04:57:06.17 Gate.power :analytic1,:ON,limit to 4000mA @04:57:06.28 Powering core bus @04:57:08.62 SPR Module 1, FW Rev. B, FPGA Rev. C @04:57:09.06 Plunge.configure :default @04:57:09.17 SamplePump.configure :default @04:57:09.27 Toroid.configure :default @04:57:09.39 Carousel.configure :default @04:57:09.49 Abs.configure :default @04:57:09.60 PhaseSeparator.configure :default @04:57:09.70 Archive.configure :default @04:57:09.81 Air.configure :default @04:57:09.89 Lysis1.configure :default @04:57:09.96 Lysis2.configure :default @04:57:10.06 PinPort.configure :default @04:57:10.13 All elf controllers running firmware version 4.92 @04:57:10.17 Gate.power :motor,:ON,limit to 5000mA @04:57:10.60 Plunge.home.jog 0 @04:57:11.19 Carousel.home.jog 0 @04:57:11.87 Lysis2.home.jog 0 @04:57:11.99 Air.home.jog 0 @04:57:12.04 Lysis1.home.jog 0 @04:57:12.08 Archive.home.jog 0 @04:57:12.14 Toroid.home.jog 0 @04:57:12.18 PinPort.home.jog 0 @04:57:13.17 Plunge.in :homing @04:57:13.49 Plunge.jog 1597 @04:57:13.58 PinPort.in :homing @04:57:13.63 Lysis1.in :homing @04:57:13.67 Toroid.in :homing @04:57:13.72 Lysis2.in :homing @04:57:13.77 Archive.in :homing @04:57:13.81 Air.in :homing @04:57:14.11 PinPort.jog -109 @04:57:14.15 Lysis1.jog 1393 @04:57:14.27 Lysis2.jog 1403 @04:57:14.31 Toroid.jog 618 @04:57:14.43 Air.jog 1363 @04:57:14.54 Archive.jog 1397 @04:57:14.94 PinPort.setRawPosition! 0 @04:57:15.01 PinPort.seek :home @04:57:15.17 PinPort.in :default @04:57:15.18 Plunge.setRawPosition! 50 @04:57:15.33 Plunge.seek :home @04:57:15.64 Plunge.in :default @04:57:15.79 Lysis1.setRawPosition! 50 @04:57:15.90 Lysis2.setRawPosition! 50 @04:57:15.92 Lysis1.seek :home @04:57:16.10 Lysis2.seek :home @04:57:16.24 Air.setRawPosition! 50 @04:57:16.29 Archive.setRawPosition! 50 @04:57:16.49 Archive.seek :home @04:57:16.52 Air.seek :home @04:57:16.75 Lysis2.in :default @04:57:16.77 Lysis1.in :default @04:57:17.00 Air.in :default @04:57:17.04 Archive.in :default @04:57:17.07 Toroid.setRawPosition! 0 @04:57:17.27 Toroid.seek :home @04:57:17.40 Toroid.in :default @04:57:17.56 Toroid.seek :clear @04:57:19.29 Carousel.in :default @04:57:19.45 Carousel.home.seek 26 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 26 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.17psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @04:57:37.79 -> ESP.shutdown @04:57:37.98 Gate.power :analytic1,:OFF @04:57:38.52 Gate.power :motor,:OFF @04:57:38.61 Gate.power :core,:OFF @04:57:38.65 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @05:04:34.47 -> ESP.ready! @05:04:34.53 Gate.power :sampler,:OFF,limit to 3500mA @05:04:34.55 Gate.power :skimmer,:OFF,limit to 3500mA @05:04:34.58 Gate.power :main,:ON,limit to 5000mA @05:04:34.63 Gate.power :analytic1,:ON,limit to 4000mA @05:04:34.73 Powering core bus @05:04:37.51 Plunge.configure :default @05:04:37.62 SamplePump.configure :default @05:04:37.73 Toroid.configure :default @05:04:37.84 Carousel.configure :default @05:04:37.95 Abs.configure :default @05:04:38.06 PhaseSeparator.configure :default @05:04:38.16 Archive.configure :default @05:04:38.28 Air.configure :default @05:04:38.40 Lysis1.configure :default @05:04:38.75 Lysis2.configure :default @05:04:38.86 PinPort.configure :default @05:04:38.93 All elf controllers running firmware version 4.92 @05:04:38.96 Gate.power :motor,:ON,limit to 5000mA @05:04:41.99 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @05:04:42.02 AnalyticModule::Unknown in bill -- SPR @05:04:42.52 Plunge.home.jog 0 @05:04:42.74 Toroid.home.jog 0 @05:04:43.26 Lysis2.home.jog 0 @05:04:43.29 Air.home.jog 0 @05:04:43.34 Carousel.home.jog 0 @05:04:43.44 PinPort.home.jog 0 @05:04:43.49 Archive.home.jog 0 @05:04:43.53 Lysis1.home.jog 0 @05:04:44.40 Toroid.in :homing @05:04:44.48 Plunge.in :homing @05:04:44.65 Toroid.jog 598 @05:04:44.68 Plunge.jog 159 @05:04:44.96 PinPort.in :homing @05:04:45.01 Lysis2.in :homing @05:04:45.05 Air.in :homing @05:04:45.10 Archive.in :homing @05:04:45.13 Lysis1.in :homing @05:04:45.38 PinPort.jog -106 @05:04:45.41 Lysis2.jog 1408 @05:04:45.48 Air.jog 1365 @05:04:45.52 Archive.jog 1397 @05:04:45.62 Lysis1.jog 1396 @05:04:46.01 PinPort.setRawPosition! 0 @05:04:46.04 Plunge.setRawPosition! 50 @05:04:46.19 Plunge.seek :home @05:04:46.22 PinPort.seek :home @05:04:46.49 PinPort.in :default @05:04:46.61 Plunge.in :default @05:04:46.75 Toroid.setRawPosition! 0 @05:04:46.84 Toroid.seek :home @05:04:46.92 Lysis2.setRawPosition! 50 @05:04:47.10 Lysis2.seek :home @05:04:47.27 Lysis1.setRawPosition! 50 @05:04:47.30 Air.setRawPosition! 50 @05:04:47.34 Archive.setRawPosition! 50 @05:04:47.39 Toroid.in :default @05:04:47.99 Air.seek :home @05:04:48.15 Lysis1.seek :home @05:04:48.19 Archive.seek :home @05:04:48.50 Lysis2.in :default @05:04:48.66 Lysis1.in :default @05:04:48.69 Air.in :default @05:04:48.86 Archive.in :default @05:04:49.23 Toroid.seek :clear @05:04:50.87 Carousel.in :default @05:04:51.02 Carousel.home.seek 26 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 26 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @05:11:04.65 -> CleanSampler.source :sampler @05:11:09.72 -> CleanSampler.source=nil @05:11:21.98 -> BACsampler.primeVolume 150 @05:11:26.37 -> BACsampler.primeVolume=100 100 @05:11:42.46 -> BACsampler.bypassVolume=10 10 @05:11:51.15 -> require 'vacuumCart' true @05:12:37.18 -> BACsampler.sourece=nil @05:12:37.22 NoMethodError in bill -- undefined method `sourece=' for BACsampler:Cartridge::Sampler @05:12:48.02 -> BACsampler.source=nil @05:12:54.42 -> slots 1..60=>nil {60..1 => nil} @05:13:01.94 -> slots 1..60=>:dry {60..1 => { Type: :unknown, State: :dry}} @05:16:58.22 -> SC.to 5 @05:16:58.26 Carousel.seek 5 Storage Carousel (SC) at 5 @05:24:54.64 -> SC.to 50 @05:24:54.67 Carousel.seek 50 Storage Carousel (SC) at 50 @05:25:17.17 -> SC.to 55 @05:25:17.22 Carousel.seek 55 Storage Carousel (SC) at 55 @05:25:39.12 -> SC.to 45 @05:25:39.16 Carousel.seek 45 Storage Carousel (SC) at 45 @05:32:43.21 -> slots 60..1=>nil {60..1 => nil} @05:33:16.91 -> slots 60..1=>dry {60..1 => { Type: :unknown, State: :dry}} @05:33:48.44 -> require 'vacuumCart' false @05:48:10.37 -> cleanToroid @05:48:10.43 Cleans Dry SC positions in slots, begin in 10% Bleach @05:48:11.25 Toroid.seek :clear @05:48:11.44 Carousel.in :default @05:48:11.57 Carousel.home.seek 45 @05:48:11.85 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @05:48:20.69 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @05:48:20.80 Priming sample loop w/100ml, bypass w/50ml @05:48:20.83 SamplePump.setPosition! 0ml @05:48:20.92 Bypass.open @05:48:22.40 Intake.open @05:48:23.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @05:48:26.30 Vacuum tare is 52% of full scale at 20C (drifted 1%) @05:48:26.50 Abs.in :default @05:48:26.58 Abs.hold @05:48:26.86 SamplePump.in :start @05:48:26.98 SamplePump.seek 50ml,3:50 @05:48:27.55 SamplePump.configure :prime @05:49:28.39 Abs.stop @05:49:28.47 SamplePump.setPosition! 0ml @05:49:28.54 Exhaust.open @05:49:29.90 Bypass.close @05:49:31.43 Abs.in :default @05:49:31.51 Abs.hold @05:49:31.84 SamplePump.in :start @05:49:31.94 SamplePump.seek 100ml,7:10 @05:49:32.53 SamplePump.configure :prime @05:51:34.32 Abs.stop @05:51:34.42 Opening all dry Cartridges @05:51:34.48 Ensuring (60) cartridge(s) open @05:51:34.79 Carousel.seek 1 @05:51:43.99 Toroid.in :hold @05:51:44.07 Toroid.seek :cartridge @05:51:45.93 Toroid.in :default @05:51:46.01 Toroid.seek :clear @05:51:48.13 Carousel.seek 2 @05:51:48.56 Toroid.in :hold @05:51:48.64 Toroid.seek :cartridge @05:51:50.51 Toroid.in :default @05:51:50.60 Toroid.seek :clear @05:51:52.70 Carousel.seek 3 @05:51:53.13 Toroid.in :hold @05:51:53.21 Toroid.seek :cartridge @05:51:55.07 Toroid.in :default @05:51:55.16 Toroid.seek :clear @05:51:57.67 Carousel.seek 4 @05:51:58.19 Toroid.in :hold @05:51:58.28 Toroid.seek :cartridge @05:52:00.16 Toroid.in :default @05:52:00.24 Toroid.seek :clear @05:52:02.49 Carousel.seek 5 @05:52:02.92 Toroid.in :hold @05:52:03.01 Toroid.seek :cartridge @05:52:04.86 Toroid.in :default @05:52:04.95 Toroid.seek :clear @05:52:07.06 Carousel.seek 6 @05:52:07.48 Toroid.in :hold @05:52:07.56 Toroid.seek :cartridge @05:52:09.42 Toroid.in :default @05:52:09.50 Toroid.seek :clear @05:52:11.60 Carousel.seek 7 @05:52:12.03 Toroid.in :hold @05:52:12.11 Toroid.seek :cartridge @05:52:13.97 Toroid.in :default @05:52:14.05 Toroid.seek :clear @05:52:16.17 Carousel.seek 8 @05:52:16.59 Toroid.in :hold @05:52:16.67 Toroid.seek :cartridge @05:52:18.53 Toroid.in :default @05:52:18.61 Toroid.seek :clear @05:52:20.72 Carousel.seek 9 @05:52:21.15 Toroid.in :hold @05:52:21.23 Toroid.seek :cartridge @05:52:23.09 Toroid.in :default @05:52:23.17 Toroid.seek :clear @05:52:25.27 Carousel.seek 10 @05:52:25.69 Toroid.in :hold @05:52:25.77 Toroid.seek :cartridge @05:52:28.06 Toroid.in :default @05:52:28.23 Toroid.seek :clear @05:52:30.35 Carousel.seek 11 @05:52:30.80 Toroid.in :hold @05:52:30.89 Toroid.seek :cartridge @05:52:32.75 Toroid.in :default @05:52:32.83 Toroid.seek :clear @05:52:34.93 Carousel.seek 12 @05:52:35.35 Toroid.in :hold @05:52:35.43 Toroid.seek :cartridge @05:52:37.30 Toroid.in :default @05:52:37.39 Toroid.seek :clear @05:52:39.49 Carousel.seek 13 @05:52:39.92 Toroid.in :hold @05:52:40.00 Toroid.seek :cartridge @05:52:41.86 Toroid.in :default @05:52:41.94 Toroid.seek :clear @05:52:44.06 Carousel.seek 14 @05:52:44.48 Toroid.in :hold @05:52:44.57 Toroid.seek :cartridge @05:52:46.43 Toroid.in :default @05:52:46.52 Toroid.seek :clear @05:52:48.64 Carousel.seek 15 @05:52:49.06 Toroid.in :hold @05:52:49.16 Toroid.seek :cartridge @05:52:51.00 Toroid.in :default @05:52:51.08 Toroid.seek :clear @05:52:53.19 Carousel.seek 16 @05:52:53.62 Toroid.in :hold @05:52:53.70 Toroid.seek :cartridge @05:52:55.57 Toroid.in :default @05:52:55.65 Toroid.seek :clear @05:52:58.15 Carousel.seek 17 @05:52:58.67 Toroid.in :hold @05:52:58.76 Toroid.seek :cartridge @05:53:00.62 Toroid.in :default @05:53:00.71 Toroid.seek :clear @05:53:02.83 Carousel.seek 18 @05:53:03.25 Toroid.in :hold @05:53:03.34 Toroid.seek :cartridge @05:53:05.19 Toroid.in :default @05:53:05.28 Toroid.seek :clear @05:53:07.41 Carousel.seek 19 @05:53:07.84 Toroid.in :hold @05:53:07.92 Toroid.seek :cartridge @05:53:09.78 Toroid.in :default @05:53:09.86 Toroid.seek :clear @05:53:11.95 Carousel.seek 20 @05:53:12.41 Toroid.in :hold @05:53:12.50 Toroid.seek :cartridge @05:53:14.39 Toroid.in :default @05:53:14.48 Toroid.seek :clear @05:53:16.62 Carousel.seek 21 @05:53:17.05 Toroid.in :hold @05:53:17.14 Toroid.seek :cartridge @05:53:18.99 Toroid.in :default @05:53:19.07 Toroid.seek :clear @05:53:21.18 Carousel.seek 22 @05:53:21.60 Toroid.in :hold @05:53:21.69 Toroid.seek :cartridge @05:53:23.55 Toroid.in :default @05:53:23.63 Toroid.seek :clear @05:53:25.75 Carousel.seek 23 @05:53:26.17 Toroid.in :hold @05:53:26.26 Toroid.seek :cartridge @05:53:28.12 Toroid.in :default @05:53:28.65 Toroid.seek :clear @05:53:30.84 Carousel.seek 24 @05:53:31.27 Toroid.in :hold @05:53:31.35 Toroid.seek :cartridge @05:53:33.19 Toroid.in :default @05:53:33.27 Toroid.seek :clear @05:53:35.38 Carousel.seek 25 @05:53:35.81 Toroid.in :hold @05:53:35.89 Toroid.seek :cartridge @05:53:37.75 Toroid.in :default @05:53:37.84 Toroid.seek :clear @05:53:39.95 Carousel.seek 26 @05:53:40.40 Toroid.in :hold @05:53:40.48 Toroid.seek :cartridge @05:53:42.35 Toroid.in :default @05:53:42.45 Toroid.seek :clear @05:53:44.61 Carousel.seek 27 @05:53:45.04 Toroid.in :hold @05:53:45.12 Toroid.seek :cartridge @05:53:46.98 Toroid.in :default @05:53:47.06 Toroid.seek :clear @05:53:49.16 Carousel.seek 28 @05:53:49.58 Toroid.in :hold @05:53:49.67 Toroid.seek :cartridge @05:53:51.53 Toroid.in :default @05:53:51.61 Toroid.seek :clear @05:53:53.72 Carousel.seek 29 @05:53:54.14 Toroid.in :hold @05:53:54.23 Toroid.seek :cartridge @05:53:56.09 Toroid.in :default @05:53:56.18 Toroid.seek :clear @05:53:58.28 Carousel.seek 30 @05:53:58.86 Toroid.in :hold @05:53:59.02 Toroid.seek :cartridge @05:54:00.88 Toroid.in :default @05:54:00.97 Toroid.seek :clear @05:54:03.09 Carousel.seek 31 @05:54:03.52 Toroid.in :hold @05:54:03.60 Toroid.seek :cartridge @05:54:05.46 Toroid.in :default @05:54:05.55 Toroid.seek :clear @05:54:07.66 Carousel.seek 32 @05:54:08.08 Toroid.in :hold @05:54:08.18 Toroid.seek :cartridge @05:54:10.03 Toroid.in :default @05:54:10.11 Toroid.seek :clear @05:54:12.22 Carousel.seek 33 @05:54:12.65 Toroid.in :hold @05:54:12.74 Toroid.seek :cartridge @05:54:14.60 Toroid.in :default @05:54:14.69 Toroid.seek :clear @05:54:16.79 Carousel.seek 34 @05:54:17.22 Toroid.in :hold @05:54:17.31 Toroid.seek :cartridge @05:54:19.17 Toroid.in :default @05:54:19.26 Toroid.seek :clear @05:54:21.36 Carousel.seek 35 @05:54:21.79 Toroid.in :hold @05:54:21.87 Toroid.seek :cartridge @05:54:23.73 Toroid.in :default @05:54:23.82 Toroid.seek :clear @05:54:25.95 Carousel.seek 36 @05:54:26.41 Toroid.in :hold @05:54:26.49 Toroid.seek :cartridge @05:54:28.37 Toroid.in :default @05:54:28.46 Toroid.seek :clear @05:54:31.00 Carousel.seek 37 @05:54:31.51 Toroid.in :hold @05:54:31.61 Toroid.seek :cartridge @05:54:33.47 Toroid.in :default @05:54:33.55 Toroid.seek :clear @05:54:35.65 Carousel.seek 38 @05:54:36.08 Toroid.in :hold @05:54:36.17 Toroid.seek :cartridge @05:54:38.02 Toroid.in :default @05:54:38.11 Toroid.seek :clear @05:54:40.21 Carousel.seek 39 @05:54:40.63 Toroid.in :hold @05:54:40.72 Toroid.seek :cartridge @05:54:42.58 Toroid.in :default @05:54:42.66 Toroid.seek :clear @05:54:44.77 Carousel.seek 40 @05:54:45.20 Toroid.in :hold @05:54:45.29 Toroid.seek :cartridge @05:54:47.15 Toroid.in :default @05:54:47.23 Toroid.seek :clear @05:54:49.33 Carousel.seek 41 @05:54:49.75 Toroid.in :hold @05:54:49.84 Toroid.seek :cartridge @05:54:51.69 Toroid.in :default @05:54:51.78 Toroid.seek :clear @05:54:53.88 Carousel.seek 42 @05:54:54.31 Toroid.in :hold @05:54:54.42 Toroid.seek :cartridge @05:54:56.28 Toroid.in :default @05:54:56.38 Toroid.seek :clear @05:54:58.53 Carousel.seek 43 @05:54:58.96 Toroid.in :hold @05:54:59.05 Toroid.seek :cartridge @05:55:01.42 Toroid.in :default @05:55:01.50 Toroid.seek :clear @05:55:03.61 Carousel.seek 44 @05:55:04.04 Toroid.in :hold @05:55:04.12 Toroid.seek :cartridge @05:55:05.99 Toroid.in :default @05:55:06.07 Toroid.seek :clear @05:55:08.18 Carousel.seek 45 @05:55:08.61 Toroid.in :hold @05:55:08.69 Toroid.seek :cartridge @05:55:10.55 Toroid.in :default @05:55:10.63 Toroid.seek :clear @05:55:12.74 Carousel.seek 46 @05:55:13.17 Toroid.in :hold @05:55:13.25 Toroid.seek :cartridge @05:55:15.11 Toroid.in :default @05:55:15.19 Toroid.seek :clear @05:55:17.30 Carousel.seek 47 @05:55:17.72 Toroid.in :hold @05:55:17.81 Toroid.seek :cartridge @05:55:19.67 Toroid.in :default @05:55:19.75 Toroid.seek :clear @05:55:21.84 Carousel.seek 48 @05:55:22.26 Toroid.in :hold @05:55:22.37 Toroid.seek :cartridge @05:55:24.24 Toroid.in :default @05:55:24.32 Toroid.seek :clear @05:55:26.47 Carousel.seek 49 @05:55:26.90 Toroid.in :hold @05:55:26.99 Toroid.seek :cartridge @05:55:28.84 Toroid.in :default @05:55:28.93 Toroid.seek :clear @05:55:31.43 Carousel.seek 50 @05:55:31.95 Toroid.in :hold @05:55:32.04 Toroid.seek :cartridge @05:55:33.90 Toroid.in :default @05:55:33.98 Toroid.seek :clear @05:55:36.08 Carousel.seek 51 @05:55:36.51 Toroid.in :hold @05:55:36.59 Toroid.seek :cartridge @05:55:38.45 Toroid.in :default @05:55:38.53 Toroid.seek :clear @05:55:40.68 Carousel.seek 52 @05:55:41.10 Toroid.in :hold @05:55:41.19 Toroid.seek :cartridge @05:55:43.04 Toroid.in :default @05:55:43.12 Toroid.seek :clear @05:55:45.23 Carousel.seek 53 @05:55:45.66 Toroid.in :hold @05:55:45.75 Toroid.seek :cartridge @05:55:47.59 Toroid.in :default @05:55:47.67 Toroid.seek :clear @05:55:49.77 Carousel.seek 54 @05:55:50.20 Toroid.in :hold @05:55:50.29 Toroid.seek :cartridge @05:55:52.18 Toroid.in :default @05:55:52.26 Toroid.seek :clear @05:55:54.39 Carousel.seek 55 @05:55:54.82 Toroid.in :hold @05:55:54.90 Toroid.seek :cartridge @05:55:56.75 Toroid.in :default @05:55:56.83 Toroid.seek :clear @05:55:58.94 Carousel.seek 56 @05:55:59.37 Toroid.in :hold @05:55:59.45 Toroid.seek :cartridge @05:56:01.31 Toroid.in :default @05:56:01.79 Toroid.seek :clear @05:56:03.99 Carousel.seek 57 @05:56:04.43 Toroid.in :hold @05:56:04.52 Toroid.seek :cartridge @05:56:06.41 Toroid.in :default @05:56:06.49 Toroid.seek :clear @05:56:08.64 Carousel.seek 58 @05:56:09.07 Toroid.in :hold @05:56:09.15 Toroid.seek :cartridge @05:56:11.01 Toroid.in :default @05:56:11.10 Toroid.seek :clear @05:56:13.20 Carousel.seek 59 @05:56:13.63 Toroid.in :hold @05:56:13.71 Toroid.seek :cartridge @05:56:15.63 Toroid.in :default @05:56:15.72 Toroid.seek :clear @05:56:17.82 Carousel.seek 60 @05:56:18.25 Toroid.in :hold @05:56:18.35 Toroid.seek :cartridge @05:56:20.22 Toroid.in :default @05:56:20.30 Toroid.seek :clear @05:56:22.17 Sampling 100.0ml @05:56:22.19 SamplePump.setPosition! 0ml @05:56:22.47 Abs.in :default @05:56:22.55 Abs.hold @05:56:22.88 SamplePump.in :start @05:56:22.98 SamplePump.seek 100ml,8:50 @05:56:23.57 SamplePump.configure :sample @06:02:22.41 Abs.stop @06:02:22.56 Intake.close @06:02:22.63 Exhaust.close @06:02:22.87 Toroid.in :hold @06:02:22.94 Toroid.seek :bypass @06:02:24.61 Toroid.in :default @06:02:24.69 Toroid.seek :clear @06:02:26.33 Sampled 100.0ml @06:02:26.42 Ensuring (60) cartridge(s) open @06:02:27.16 Carousel.seek 60 @06:02:27.39 Toroid.in :hold @06:02:27.47 Toroid.seek :cartridge @06:02:29.35 Toroid.in :default @06:02:29.43 Toroid.seek :clear @06:02:31.26 Sample loop filled with Bleach. Move intake to clean water source @06:02:31.29 delay 15:00 @06:03:17.99 UserAbort in bill -- User Abort!! @06:17:58.75 -> cleanToroid @06:17:58.77 Cleans Dry SC positions in slots, begin in 10% Bleach @06:17:59.60 Toroid.seek :clear @06:17:59.80 Carousel.in :default @06:17:59.94 Carousel.home.seek 60 @06:18:00.25 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @06:18:02.64 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @06:18:02.77 Toroid.in :hold @06:18:02.85 Toroid.seek :bypass @06:18:04.52 Toroid.in :default @06:18:04.60 Toroid.seek :clear @06:18:06.25 Priming sample loop w/100ml, bypass w/50ml @06:18:06.27 SamplePump.setPosition! 0ml @06:18:06.46 Bypass.open @06:18:08.02 Intake.open @06:18:09.56 Abs.in :default @06:18:09.64 Abs.hold @06:18:09.92 SamplePump.in :start @06:18:10.01 SamplePump.seek 50ml,3:50 @06:18:10.61 SamplePump.configure :prime @06:19:11.39 Abs.stop @06:19:11.48 SamplePump.setPosition! 0ml @06:19:11.56 Exhaust.open @06:19:12.95 Bypass.close @06:19:14.52 Abs.in :default @06:19:14.60 Abs.hold @06:19:14.94 SamplePump.in :start @06:19:15.04 SamplePump.seek 100ml,7:10 @06:19:15.64 SamplePump.configure :prime @06:21:54.32 Abs.stop @06:21:54.42 Opening all dry Cartridges @06:21:54.48 Ensuring (60) cartridge(s) open @06:21:54.79 Carousel.seek 60 @06:21:55.39 Toroid.in :hold @06:21:55.56 Toroid.seek :cartridge @06:21:57.42 Toroid.in :default @06:21:57.51 Toroid.seek :clear @06:21:59.35 Sampling 100.0ml @06:21:59.38 SamplePump.setPosition! 0ml @06:21:59.66 Abs.in :default @06:21:59.74 Abs.hold @06:22:00.07 SamplePump.in :start @06:22:00.17 SamplePump.seek 100ml,8:50 @06:22:00.77 SamplePump.configure :sample @06:28:03.97 Abs.stop @06:28:04.13 Intake.close @06:28:04.25 Exhaust.close @06:28:04.61 Toroid.in :hold @06:28:04.71 Toroid.seek :bypass @06:28:06.39 Toroid.in :default @06:28:06.49 Toroid.seek :clear @06:28:08.14 Sampled 100.0ml @06:28:08.20 Ensuring (60) cartridge(s) open @06:28:08.56 Carousel.seek 60 @06:28:08.70 Toroid.in :hold @06:28:08.78 Toroid.seek :cartridge @06:28:10.65 Toroid.in :default @06:28:10.73 Toroid.seek :clear @06:28:12.56 Sample loop filled with Bleach. Move intake to clean water source @06:28:12.59 delay 15:00 @06:43:12.72 Toroid.in :hold @06:43:12.80 Toroid.seek :bypass @06:43:14.46 Toroid.in :default @06:43:14.55 Toroid.seek :clear @06:43:16.19 Priming bypass w/50ml @06:43:16.21 SamplePump.setPosition! 0ml @06:43:16.31 Bypass.open @06:43:17.91 Intake.open @06:43:19.47 Abs.in :default @06:43:19.57 Abs.hold @06:43:19.85 SamplePump.in :start @06:43:19.94 SamplePump.seek 50ml,3:50 @06:43:20.54 SamplePump.configure :prime @06:44:21.31 Abs.stop @06:44:21.46 Ensuring (60) cartridge(s) open @06:44:21.79 Carousel.seek 60 @06:44:22.44 Toroid.in :hold @06:44:22.62 Toroid.seek :cartridge @06:44:24.48 Toroid.in :default @06:44:24.55 Toroid.seek :clear @06:44:26.41 Sampling 500.0ml @06:44:26.43 SamplePump.setPosition! 0ml @06:44:26.54 Bypass.close @06:44:26.63 Exhaust.open @06:44:27.47 Abs.in :default @06:44:27.56 Abs.hold @06:44:27.90 SamplePump.in :start @06:44:28.00 SamplePump.seek 500ml,42:10 @06:44:28.58 SamplePump.configure :sample @07:00:25.57 SamplePump.stop @07:00:25.66 Intake.close @07:00:25.76 Exhaust.close @07:00:26.04 Toroid.in :hold @07:00:26.13 Toroid.seek :bypass @07:00:27.81 Toroid.in :default @07:00:27.90 Toroid.seek :clear @07:00:29.54 Abs.stop @07:00:29.80 Cartridge::Sampler::Leaky in bill -- Serious Leak or Stall -- 0.25ml/s made only -36.1psi vacuum after 283.1ml @07:01:34.58 -> Leak.psi @07:01:34.64 NoMethodError in bill -- undefined method `psi' for Abs:PressureSensor @07:01:38.23 -> Leak.psia 14.1263919910542 @07:04:30.98 -> BACsampler.prime 100,nil;loop{puts Leak.psia;Delay.sleep 0.5} @07:04:31.09 Priming sample loop w/100ml @07:04:31.11 SamplePump.setPosition! 0ml @07:04:31.21 Bypass.open @07:04:32.79 Exhaust.open @07:04:34.18 Bypass.close @07:04:34.21 Intake.open @07:04:35.83 Abs.in :default @07:04:35.91 Abs.hold @07:04:36.26 SamplePump.in :start @07:04:36.38 SamplePump.seek 100ml,7:10 @07:04:36.95 SamplePump.configure :prime @07:05:07.31 Session Begun @07:05:18.11 -> loop{puts Leak.psia;Delay.sleep 0.5} @07:07:14.54 UserAbort in n -- User Abort!! @07:07:16.64 Abs.stop @07:07:34.51 -> Intake.open;Exhaust.open Exhaust is opened @07:07:45.21 -> SP.volume=0 @07:07:45.24 SamplePump.setPosition! 0ml 0 @07:08:12.38 -> SP.pump 100,slow;loop{puts Leak.psia;Delay.sleep 0.5} @07:08:12.60 SamplePump.in :slow @07:08:12.69 SamplePump.seek 100ml @07:15:19.76 UserAbort in n -- User Abort!! @07:15:24.67 UserAbort in bill -- User Abort!! @07:17:03.03 -> slots 1..60=>dry {59..1 => { Type: :unknown, State: :dry, TV: :open}, 60 => { Type: :unknown, State: :dry}} @07:18:33.13 -> vacToroid @07:18:33.21 Vacuuming protocol for dry cartridges @07:18:33.95 Toroid.seek :clear @07:18:34.17 Carousel.in :default @07:18:34.32 Carousel.home.seek 60 @07:18:34.67 Waiting for bill to answer "Disconnect the intake line. push return" @07:18:46.64 Answered Disconnect the intake line. push return"" @07:18:47.42 Toroid.seek :clear @07:18:47.65 Carousel.in :default @07:18:47.79 Carousel.home.seek 60 @07:18:48.09 SamplePump.setPosition! 0ml @07:18:48.17 SamplePump.in :default @07:18:48.27 SamplePump.seek 50ml @07:19:49.91 Exhaust.close @07:19:50.62 Bypass.open @07:19:51.37 SamplePump.seek 75ml @07:20:22.50 Intake.close @07:20:23.25 Bypass.close @07:20:23.96 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @07:21:05.59 -> PB.open @07:21:05.62 Bypass.open Bypass (PB) is opened @07:21:10.51 -> PB.close @07:21:10.54 Bypass.close Bypass (PB) is closed @07:21:16.40 -> PB.open @07:21:16.43 Bypass.open Bypass (PB) is opened @07:21:19.88 -> PB.close @07:21:19.91 Bypass.close Bypass (PB) is closed @07:21:40.82 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @07:21:40.85 Exhaust.open @07:21:41.59 Bypass.open @07:21:42.31 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @07:21:48.81 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @07:21:48.85 delay 30 seconds @07:22:18.99 Clearing fluid from cartridge-side of Toroid @07:22:19.02 delay 2 seconds @07:22:21.15 delay 2 seconds @07:22:23.24 delay 2 seconds @07:22:25.33 delay 2 seconds @07:22:27.42 delay 2 seconds @07:22:29.51 delay 2 seconds @07:22:31.60 delay 2 seconds @07:22:33.69 delay 2 seconds @07:22:35.77 delay 2 seconds @07:22:37.86 delay 2 seconds @07:22:39.95 delay 2 seconds @07:22:42.04 delay 2 seconds @07:22:44.13 delay 2 seconds @07:22:46.22 delay 2 seconds @07:22:48.32 delay 2 seconds @07:22:50.45 delay 2 seconds @07:22:53.14 delay 2 seconds @07:22:55.27 delay 2 seconds @07:22:57.36 delay 2 seconds @07:22:59.46 delay 2 seconds @07:23:01.54 delay 2 seconds @07:23:03.63 delay 2 seconds @07:23:05.72 delay 2 seconds @07:23:07.81 delay 2 seconds @07:23:09.90 delay 2 seconds @07:23:11.99 delay 2 seconds @07:23:14.08 delay 2 seconds @07:23:16.16 delay 2 seconds @07:23:18.25 delay 2 seconds @07:23:20.35 delay 2 seconds @07:23:22.45 delay 2 seconds @07:23:24.54 delay 2 seconds @07:23:26.63 delay 2 seconds @07:23:28.74 delay 2 seconds @07:23:30.85 delay 2 seconds @07:23:32.94 delay 2 seconds @07:23:35.03 delay 2 seconds @07:23:37.12 delay 2 seconds @07:23:39.21 delay 2 seconds @07:23:41.29 delay 2 seconds @07:23:43.38 delay 2 seconds @07:23:45.47 delay 2 seconds @07:23:47.56 delay 2 seconds @07:23:49.66 delay 2 seconds @07:23:51.75 delay 2 seconds @07:23:53.84 delay 2 seconds @07:23:55.92 delay 2 seconds @07:23:58.01 delay 2 seconds @07:24:00.10 delay 2 seconds @07:24:02.19 delay 2 seconds @07:24:04.28 delay 2 seconds @07:24:06.40 delay 2 seconds @07:24:08.48 delay 2 seconds @07:24:10.57 delay 2 seconds @07:24:12.66 delay 2 seconds @07:24:14.75 delay 2 seconds @07:24:16.84 delay 2 seconds @07:24:18.92 delay 2 seconds @07:24:21.01 delay 2 seconds @07:24:23.36 Carousel.seek 60 @07:24:23.52 Toroid.in :hold @07:24:23.61 Toroid.seek :cartridge @07:24:25.49 Toroid.in :default @07:24:25.59 Toroid.seek :clear @07:24:27.43 delay 2 seconds @07:24:29.54 Toroid.in :hold @07:24:29.64 Toroid.seek :bypass @07:24:31.31 Toroid.in :default @07:24:31.42 Toroid.seek :clear @07:24:33.07 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @07:29:21.19 UserAbort in bill -- User Abort!! @07:31:55.69 -> TV.cartridge @07:31:55.85 Toroid.in :hold @07:31:55.96 Toroid.seek :cartridge @07:31:57.85 Toroid.in :default @07:31:57.94 Toroid.seek :clear Toroid Valve (TV) clear @07:32:01.91 -> SC Storage Carousel (SC) at 60 @07:32:22.09 -> Exhaust.open;PB.open Bypass (PB) is opened @07:32:30.66 -> Leak.psia 2.05929386738796 @07:32:47.53 -> PB.close;Exhaust.close @07:32:47.56 Bypass.close @07:32:48.29 Exhaust.close Exhaust is closed @07:37:50.55 -> SC.to 40 @07:37:50.60 Carousel.seek 40 Storage Carousel (SC) at 40 @07:40:24.25 -> SC.to 22 @07:40:24.29 Carousel.seek 22 Storage Carousel (SC) at 22 @07:40:42.89 -> BACsampler.prime 150,50 @07:40:43.05 Toroid.in :hold @07:40:43.15 Toroid.seek :bypass @07:40:44.83 Toroid.in :default @07:40:44.93 Toroid.seek :clear @07:40:46.59 Priming sample loop w/150ml, bypass w/50ml @07:40:46.62 SamplePump.setPosition! 0ml @07:40:46.74 Bypass.open @07:40:48.29 Intake.open @07:40:49.90 Abs.in :default @07:40:49.99 Abs.hold @07:40:50.28 SamplePump.in :start @07:40:50.42 SamplePump.seek 50ml,3:50 @07:40:50.97 SamplePump.configure :prime @07:40:59.70 -> loop{puts Leak.psia;Delay.sleep .5} @07:41:33.56 UserAbort in n -- User Abort!! @07:41:36.12 SamplePump.hold @07:41:36.41 Intake.close @07:41:36.51 Bypass.close @07:41:37.35 Abs.stop @07:41:37.49 UserAbort in bill -- Sample Pump (SP) seek to 50ml Aborted! @07:41:53.85 -> SC.to 60 @07:41:53.91 Carousel.seek 60 Storage Carousel (SC) at 60 @07:42:17.75 -> SC.to 22 @07:42:17.79 Carousel.seek 22 Storage Carousel (SC) at 22 @07:42:50.42 -> Cartridge.bypassAll @07:42:50.48 Ensuring all (59) cartridges are bypassed @07:42:50.84 Carousel.seek 1 @07:42:55.37 Toroid.in :hold @07:42:55.48 Toroid.seek :bypass @07:42:57.16 Toroid.in :default @07:42:57.27 Toroid.seek :clear @07:42:59.20 Carousel.seek 2 @07:42:59.66 Toroid.in :hold @07:42:59.76 Toroid.seek :bypass @07:43:01.43 Toroid.in :default @07:43:01.54 Toroid.seek :clear @07:43:03.46 Carousel.seek 3 @07:43:03.90 Toroid.in :hold @07:43:03.99 Toroid.seek :bypass @07:43:05.67 Toroid.in :default @07:43:05.78 Toroid.seek :clear @07:43:08.40 Carousel.seek 4 @07:43:08.85 Toroid.in :hold @07:43:08.96 Toroid.seek :bypass @07:43:10.62 Toroid.in :default @07:43:10.73 Toroid.seek :clear @07:43:12.68 Carousel.seek 5 @07:43:13.13 Toroid.in :hold @07:43:13.24 Toroid.seek :bypass @07:43:14.91 Toroid.in :default @07:43:15.02 Toroid.seek :clear @07:43:16.95 Carousel.seek 6 @07:43:17.40 Toroid.in :hold @07:43:17.51 Toroid.seek :bypass @07:43:19.18 Toroid.in :default @07:43:19.29 Toroid.seek :clear @07:43:21.22 Carousel.seek 7 @07:43:21.68 Toroid.in :hold @07:43:21.79 Toroid.seek :bypass @07:43:23.48 Toroid.in :default @07:43:23.59 Toroid.seek :clear @07:43:25.54 Carousel.seek 8 @07:43:26.00 Toroid.in :hold @07:43:26.10 Toroid.seek :bypass @07:43:27.78 Toroid.in :default @07:43:27.89 Toroid.seek :clear @07:43:29.81 Carousel.seek 9 @07:43:30.26 Toroid.in :hold @07:43:30.39 Toroid.seek :bypass @07:43:32.07 Toroid.in :default @07:43:32.17 Toroid.seek :clear @07:43:34.10 Carousel.seek 10 @07:43:34.55 Toroid.in :hold @07:43:34.65 Toroid.seek :bypass @07:43:36.87 Toroid.in :default @07:43:37.08 Toroid.seek :clear @07:43:39.00 Carousel.seek 11 @07:43:39.46 Toroid.in :hold @07:43:39.56 Toroid.seek :bypass @07:43:41.25 Toroid.in :default @07:43:41.35 Toroid.seek :clear @07:43:43.26 Carousel.seek 12 @07:43:43.71 Toroid.in :hold @07:43:43.82 Toroid.seek :bypass @07:43:45.50 Toroid.in :default @07:43:45.62 Toroid.seek :clear @07:43:47.54 Carousel.seek 13 @07:43:47.99 Toroid.in :hold @07:43:48.09 Toroid.seek :bypass @07:43:49.78 Toroid.in :default @07:43:49.88 Toroid.seek :clear @07:43:51.81 Carousel.seek 14 @07:43:52.26 Toroid.in :hold @07:43:52.39 Toroid.seek :bypass @07:43:54.08 Toroid.in :default @07:43:54.17 Toroid.seek :clear @07:43:56.12 Carousel.seek 15 @07:43:56.57 Toroid.in :hold @07:43:56.67 Toroid.seek :bypass @07:43:58.44 Toroid.in :default @07:43:58.55 Toroid.seek :clear @07:44:00.49 Carousel.seek 16 @07:44:00.95 Toroid.in :hold @07:44:01.06 Toroid.seek :bypass @07:44:02.74 Toroid.in :default @07:44:02.84 Toroid.seek :clear @07:44:05.32 Carousel.seek 17 @07:44:05.87 Toroid.in :hold @07:44:05.97 Toroid.seek :bypass @07:44:07.68 Toroid.in :default @07:44:07.78 Toroid.seek :clear @07:44:09.73 Carousel.seek 18 @07:44:10.19 Toroid.in :hold @07:44:10.30 Toroid.seek :bypass @07:44:12.01 Toroid.in :default @07:44:12.13 Toroid.seek :clear @07:44:14.07 Carousel.seek 19 @07:44:14.49 Toroid.in :hold @07:44:14.60 Toroid.seek :bypass @07:44:16.28 Toroid.in :default @07:44:16.41 Toroid.seek :clear @07:44:18.37 Carousel.seek 20 @07:44:18.83 Toroid.in :hold @07:44:18.94 Toroid.seek :bypass @07:44:20.63 Toroid.in :default @07:44:20.74 Toroid.seek :clear @07:44:22.69 Carousel.seek 21 @07:44:23.14 Toroid.in :hold @07:44:23.24 Toroid.seek :bypass @07:44:24.92 Toroid.in :default @07:44:25.04 Toroid.seek :clear @07:44:26.97 Carousel.seek 23 @07:44:27.62 Toroid.in :hold @07:44:27.73 Toroid.seek :bypass @07:44:29.40 Toroid.in :default @07:44:29.51 Toroid.seek :clear @07:44:31.44 Carousel.seek 24 @07:44:31.90 Toroid.in :hold @07:44:32.01 Toroid.seek :bypass @07:44:33.69 Toroid.in :default @07:44:34.33 Toroid.seek :clear @07:44:36.44 Carousel.seek 25 @07:44:36.89 Toroid.in :hold @07:44:36.99 Toroid.seek :bypass @07:44:38.67 Toroid.in :default @07:44:38.77 Toroid.seek :clear @07:44:40.71 Carousel.seek 26 @07:44:41.16 Toroid.in :hold @07:44:41.27 Toroid.seek :bypass @07:44:42.95 Toroid.in :default @07:44:43.04 Toroid.seek :clear @07:44:44.98 Carousel.seek 27 @07:44:45.43 Toroid.in :hold @07:44:45.54 Toroid.seek :bypass @07:44:47.22 Toroid.in :default @07:44:47.32 Toroid.seek :clear @07:44:49.25 Carousel.seek 28 @07:44:49.70 Toroid.in :hold @07:44:49.80 Toroid.seek :bypass @07:44:51.48 Toroid.in :default @07:44:51.59 Toroid.seek :clear @07:44:53.53 Carousel.seek 29 @07:44:53.98 Toroid.in :hold @07:44:54.08 Toroid.seek :bypass @07:44:55.77 Toroid.in :default @07:44:55.88 Toroid.seek :clear @07:44:57.81 Carousel.seek 30 @07:44:58.24 Toroid.in :hold @07:44:58.36 Toroid.seek :bypass @07:45:00.06 Toroid.in :default @07:45:00.18 Toroid.seek :clear @07:45:02.10 Carousel.seek 31 @07:45:03.15 Toroid.in :hold @07:45:03.35 Toroid.seek :bypass @07:45:05.03 Toroid.in :default @07:45:05.13 Toroid.seek :clear @07:45:07.07 Carousel.seek 32 @07:45:07.51 Toroid.in :hold @07:45:07.63 Toroid.seek :bypass @07:45:09.30 Toroid.in :default @07:45:09.41 Toroid.seek :clear @07:45:11.35 Carousel.seek 33 @07:45:11.80 Toroid.in :hold @07:45:11.90 Toroid.seek :bypass @07:45:13.58 Toroid.in :default @07:45:13.68 Toroid.seek :clear @07:45:15.61 Carousel.seek 34 @07:45:16.06 Toroid.in :hold @07:45:16.16 Toroid.seek :bypass @07:45:17.86 Toroid.in :default @07:45:17.95 Toroid.seek :clear @07:45:19.86 Carousel.seek 35 @07:45:20.32 Toroid.in :hold @07:45:20.45 Toroid.seek :bypass @07:45:22.14 Toroid.in :default @07:45:22.24 Toroid.seek :clear @07:45:24.17 Carousel.seek 36 @07:45:24.62 Toroid.in :hold @07:45:24.72 Toroid.seek :bypass @07:45:26.44 Toroid.in :default @07:45:26.55 Toroid.seek :clear @07:45:28.51 Carousel.seek 37 @07:45:28.96 Toroid.in :hold @07:45:29.07 Toroid.seek :bypass @07:45:30.75 Toroid.in :default @07:45:30.85 Toroid.seek :clear @07:45:33.35 Carousel.seek 38 @07:45:33.89 Toroid.in :hold @07:45:33.99 Toroid.seek :bypass @07:45:35.67 Toroid.in :default @07:45:35.78 Toroid.seek :clear @07:45:37.71 Carousel.seek 39 @07:45:38.16 Toroid.in :hold @07:45:38.26 Toroid.seek :bypass @07:45:39.94 Toroid.in :default @07:45:40.06 Toroid.seek :clear @07:45:41.99 Carousel.seek 40 @07:45:42.45 Toroid.in :hold @07:45:42.55 Toroid.seek :bypass @07:45:44.23 Toroid.in :default @07:45:44.33 Toroid.seek :clear @07:45:46.30 Carousel.seek 41 @07:45:46.73 Toroid.in :hold @07:45:46.84 Toroid.seek :bypass @07:45:48.52 Toroid.in :default @07:45:48.61 Toroid.seek :clear @07:45:50.59 Carousel.seek 42 @07:45:51.04 Toroid.in :hold @07:45:51.13 Toroid.seek :bypass @07:45:52.82 Toroid.in :default @07:45:52.92 Toroid.seek :clear @07:45:54.86 Carousel.seek 43 @07:45:55.31 Toroid.in :hold @07:45:55.42 Toroid.seek :bypass @07:45:57.09 Toroid.in :default @07:45:57.20 Toroid.seek :clear @07:45:59.18 Carousel.seek 44 @07:45:59.64 Toroid.in :hold @07:46:00.29 Toroid.seek :bypass @07:46:02.10 Toroid.in :default @07:46:02.20 Toroid.seek :clear @07:46:04.14 Carousel.seek 45 @07:46:04.59 Toroid.in :hold @07:46:04.70 Toroid.seek :bypass @07:46:06.40 Toroid.in :default @07:46:06.52 Toroid.seek :clear @07:46:08.50 Carousel.seek 46 @07:46:08.95 Toroid.in :hold @07:46:09.07 Toroid.seek :bypass @07:46:10.75 Toroid.in :default @07:46:10.86 Toroid.seek :clear @07:46:12.81 Carousel.seek 47 @07:46:13.26 Toroid.in :hold @07:46:13.36 Toroid.seek :bypass @07:46:15.05 Toroid.in :default @07:46:15.15 Toroid.seek :clear @07:46:17.10 Carousel.seek 48 @07:46:17.56 Toroid.in :hold @07:46:17.68 Toroid.seek :bypass @07:46:19.34 Toroid.in :default @07:46:19.45 Toroid.seek :clear @07:46:21.41 Carousel.seek 49 @07:46:21.86 Toroid.in :hold @07:46:21.97 Toroid.seek :bypass @07:46:23.66 Toroid.in :default @07:46:23.77 Toroid.seek :clear @07:46:25.69 Carousel.seek 50 @07:46:26.14 Toroid.in :hold @07:46:26.25 Toroid.seek :bypass @07:46:27.93 Toroid.in :default @07:46:28.04 Toroid.seek :clear @07:46:30.52 Carousel.seek 51 @07:46:31.07 Toroid.in :hold @07:46:31.16 Toroid.seek :bypass @07:46:32.85 Toroid.in :default @07:46:32.96 Toroid.seek :clear @07:46:34.90 Carousel.seek 52 @07:46:35.35 Toroid.in :hold @07:46:35.45 Toroid.seek :bypass @07:46:37.14 Toroid.in :default @07:46:37.25 Toroid.seek :clear @07:46:39.18 Carousel.seek 53 @07:46:39.64 Toroid.in :hold @07:46:39.73 Toroid.seek :bypass @07:46:41.42 Toroid.in :default @07:46:41.51 Toroid.seek :clear @07:46:43.43 Carousel.seek 54 @07:46:43.88 Toroid.in :hold @07:46:43.99 Toroid.seek :bypass @07:46:45.66 Toroid.in :default @07:46:45.76 Toroid.seek :clear @07:46:47.68 Carousel.seek 55 @07:46:48.14 Toroid.in :hold @07:46:48.24 Toroid.seek :bypass @07:46:49.93 Toroid.in :default @07:46:50.03 Toroid.seek :clear @07:46:51.95 Carousel.seek 56 @07:46:52.43 Toroid.in :hold @07:46:52.54 Toroid.seek :bypass @07:46:54.23 Toroid.in :default @07:46:54.36 Toroid.seek :clear @07:46:56.31 Carousel.seek 57 @07:46:56.80 Toroid.in :hold @07:46:56.91 Toroid.seek :bypass @07:46:59.10 Toroid.in :default @07:46:59.31 Toroid.seek :clear @07:47:01.26 Carousel.seek 58 @07:47:01.70 Toroid.in :hold @07:47:01.80 Toroid.seek :bypass @07:47:03.48 Toroid.in :default @07:47:03.58 Toroid.seek :clear @07:47:05.49 Carousel.seek 59 @07:47:05.94 Toroid.in :hold @07:47:06.05 Toroid.seek :bypass @07:47:07.73 Toroid.in :default @07:47:07.84 Toroid.seek :clear @07:47:09.77 Carousel.seek 60 @07:47:10.22 Toroid.in :hold @07:47:10.33 Toroid.seek :bypass @07:47:12.05 Toroid.in :default @07:47:12.15 Toroid.seek :clear [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60] @07:47:50.79 -> BACsampler.prime 100,20;PhytoSampler.prime 100,20 @07:47:50.92 Priming sample loop w/100ml, bypass w/20ml @07:47:50.94 SamplePump.setPosition! 0ml @07:47:51.06 Bypass.open @07:47:52.66 Intake.open @07:47:54.25 Abs.in :default @07:47:54.37 Abs.hold @07:47:54.66 SamplePump.in :start @07:47:54.77 SamplePump.seek 20ml,1:50 @07:47:55.35 SamplePump.configure :prime @07:48:19.55 Abs.stop @07:48:19.64 SamplePump.setPosition! 0ml @07:48:19.74 Exhaust.open @07:48:21.12 Bypass.close @07:48:22.73 Abs.in :default @07:48:22.82 Abs.hold @07:48:23.18 SamplePump.in :start @07:48:23.29 SamplePump.seek 100ml,7:10 @07:48:23.86 SamplePump.configure :prime @07:49:40.05 -> loop{puts Leak.psia;Delay.sleep 0.5} @07:50:25.65 Abs.stop @07:50:25.83 Priming sample loop w/100ml, bypass w/20ml @07:50:25.85 SamplePump.setPosition! 0ml @07:50:25.96 Exhaust.close @07:50:26.63 Bypass.open @07:50:28.44 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:50:31.18 Vacuum tare is 52% of full scale at 21C (drifted 1%) @07:50:31.37 Abs.in :default @07:50:31.46 Abs.hold @07:50:31.89 SamplePump.in :start @07:50:32.01 SamplePump.seek 20ml,1:50 @07:50:32.58 SamplePump.configure :prime @07:50:56.79 Abs.stop @07:50:56.89 SamplePump.setPosition! 0ml @07:50:56.97 Exhaust.open @07:50:58.38 Bypass.close @07:51:00.26 Abs.in :default @07:51:00.39 Abs.hold @07:51:00.90 SamplePump.in :start @07:51:01.02 SamplePump.seek 100ml,7:10 @07:51:01.59 SamplePump.configure :prime @07:53:03.41 Abs.stop PhytoSampler @07:53:18.11 UserAbort in n -- User Abort!! @07:54:26.82 -> vacToroird :vacToroird @07:54:40.01 -> vacToroid @07:54:40.09 Vacuuming protocol for dry cartridges @07:54:40.99 Toroid.seek :clear @07:54:41.21 Carousel.in :default @07:54:41.38 Carousel.home.seek 60 @07:54:41.68 Waiting for bill to answer "Disconnect the intake line. push return" @07:54:46.66 Answered Disconnect the intake line. push return"" @07:54:47.51 Toroid.seek :clear @07:54:47.73 Carousel.in :default @07:54:47.88 Carousel.home.seek 60 @07:54:48.18 SamplePump.setPosition! 0ml @07:54:48.26 SamplePump.in :default @07:54:48.37 SamplePump.seek 50ml @07:55:50.01 Exhaust.close @07:55:50.67 Bypass.open @07:55:51.40 SamplePump.seek 75ml @07:56:22.52 Intake.close @07:56:23.30 Bypass.close @07:56:24.00 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @07:56:44.84 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @07:56:44.85 Exhaust.open @07:56:45.57 Bypass.open @07:56:46.26 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @07:56:54.26 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @07:56:54.28 delay 30 seconds @07:57:24.38 Clearing fluid from cartridge-side of Toroid @07:57:24.71 Carousel.seek 1 @07:57:36.93 Toroid.in :hold @07:57:37.04 Toroid.seek :cartridge @07:57:38.92 Toroid.in :default @07:57:39.03 Toroid.seek :clear @07:57:40.88 delay 2 seconds @07:57:42.99 Toroid.in :hold @07:57:43.09 Toroid.seek :bypass @07:57:44.77 Toroid.in :default @07:57:44.88 Toroid.seek :clear @07:57:46.80 Carousel.seek 2 @07:57:47.25 Toroid.in :hold @07:57:47.35 Toroid.seek :cartridge @07:57:49.23 Toroid.in :default @07:57:49.33 Toroid.seek :clear @07:57:51.84 delay 2 seconds @07:57:53.96 Toroid.in :hold @07:57:54.07 Toroid.seek :bypass @07:57:55.74 Toroid.in :default @07:57:55.84 Toroid.seek :clear @07:57:57.77 Carousel.seek 3 @07:57:58.22 Toroid.in :hold @07:57:58.32 Toroid.seek :cartridge @07:58:00.23 Toroid.in :default @07:58:00.34 Toroid.seek :clear @07:58:02.22 delay 2 seconds @07:58:04.34 Toroid.in :hold @07:58:04.48 Toroid.seek :bypass @07:58:06.15 Toroid.in :default @07:58:06.25 Toroid.seek :clear @07:58:08.19 Carousel.seek 4 @07:58:08.64 Toroid.in :hold @07:58:08.74 Toroid.seek :cartridge @07:58:10.61 Toroid.in :default @07:58:10.71 Toroid.seek :clear @07:58:12.57 delay 2 seconds @07:58:14.70 Toroid.in :hold @07:58:14.79 Toroid.seek :bypass @07:58:16.45 Toroid.in :default @07:58:16.55 Toroid.seek :clear @07:58:18.52 Carousel.seek 5 @07:58:18.98 Toroid.in :hold @07:58:19.08 Toroid.seek :cartridge @07:58:20.95 Toroid.in :default @07:58:21.06 Toroid.seek :clear @07:58:22.91 delay 2 seconds @07:58:25.03 Toroid.in :hold @07:58:25.13 Toroid.seek :bypass @07:58:26.80 Toroid.in :default @07:58:26.90 Toroid.seek :clear @07:58:28.83 Carousel.seek 6 @07:58:29.28 Toroid.in :hold @07:58:29.39 Toroid.seek :cartridge @07:58:31.27 Toroid.in :default @07:58:31.38 Toroid.seek :clear @07:58:33.23 delay 2 seconds @07:58:35.43 Toroid.in :hold @07:58:35.54 Toroid.seek :bypass @07:58:37.20 Toroid.in :default @07:58:37.31 Toroid.seek :clear @07:58:39.21 Carousel.seek 7 @07:58:39.66 Toroid.in :hold @07:58:39.77 Toroid.seek :cartridge @07:58:41.64 Toroid.in :default @07:58:41.74 Toroid.seek :clear @07:58:43.58 delay 2 seconds @07:58:45.70 Toroid.in :hold @07:58:45.80 Toroid.seek :bypass @07:58:47.48 Toroid.in :default @07:58:47.58 Toroid.seek :clear @07:58:49.51 Carousel.seek 8 @07:58:49.96 Toroid.in :hold @07:58:50.06 Toroid.seek :cartridge @07:58:51.94 Toroid.in :default @07:58:52.05 Toroid.seek :clear @07:58:53.90 delay 2 seconds @07:58:56.01 Toroid.in :hold @07:58:56.11 Toroid.seek :bypass @07:58:57.79 Toroid.in :default @07:58:57.89 Toroid.seek :clear @07:58:59.79 Carousel.seek 9 @07:59:00.26 Toroid.in :hold @07:59:00.42 Toroid.seek :cartridge @07:59:02.32 Toroid.in :default @07:59:02.46 Toroid.seek :clear @07:59:04.30 delay 2 seconds @07:59:06.45 Toroid.in :hold @07:59:06.55 Toroid.seek :bypass @07:59:08.21 Toroid.in :default @07:59:08.32 Toroid.seek :clear @07:59:10.28 Carousel.seek 10 @07:59:10.77 Toroid.in :hold @07:59:10.86 Toroid.seek :cartridge @07:59:12.74 Toroid.in :default @07:59:12.84 Toroid.seek :clear @07:59:14.68 delay 2 seconds @07:59:17.34 Toroid.in :hold @07:59:17.53 Toroid.seek :bypass @07:59:19.20 Toroid.in :default @07:59:19.30 Toroid.seek :clear @07:59:21.25 Carousel.seek 11 @07:59:21.71 Toroid.in :hold @07:59:21.83 Toroid.seek :cartridge @07:59:23.70 Toroid.in :default @07:59:23.79 Toroid.seek :clear @07:59:25.65 delay 2 seconds @07:59:27.76 Toroid.in :hold @07:59:27.86 Toroid.seek :bypass @07:59:29.54 Toroid.in :default @07:59:29.64 Toroid.seek :clear @07:59:31.57 Carousel.seek 12 @07:59:32.00 Toroid.in :hold @07:59:32.11 Toroid.seek :cartridge @07:59:33.99 Toroid.in :default @07:59:34.08 Toroid.seek :clear @07:59:35.94 delay 2 seconds @07:59:38.05 Toroid.in :hold @07:59:38.15 Toroid.seek :bypass @07:59:39.82 Toroid.in :default @07:59:39.93 Toroid.seek :clear @07:59:41.85 Carousel.seek 13 @07:59:42.29 Toroid.in :hold @07:59:42.44 Toroid.seek :cartridge @07:59:44.31 Toroid.in :default @07:59:44.45 Toroid.seek :clear @07:59:46.29 delay 2 seconds @07:59:48.44 Toroid.in :hold @07:59:48.55 Toroid.seek :bypass @07:59:50.22 Toroid.in :default @07:59:50.32 Toroid.seek :clear @07:59:52.30 Carousel.seek 14 @07:59:52.78 Toroid.in :hold @07:59:52.88 Toroid.seek :cartridge @07:59:54.74 Toroid.in :default @07:59:54.85 Toroid.seek :clear @07:59:56.70 delay 2 seconds @07:59:59.36 Toroid.in :hold @07:59:59.55 Toroid.seek :bypass @08:00:01.25 Toroid.in :default @08:00:01.36 Toroid.seek :clear @08:00:03.29 Carousel.seek 15 @08:00:03.75 Toroid.in :hold @08:00:03.85 Toroid.seek :cartridge @08:00:05.72 Toroid.in :default @08:00:05.82 Toroid.seek :clear @08:00:07.67 delay 2 seconds @08:00:09.78 Toroid.in :hold @08:00:09.89 Toroid.seek :bypass @08:00:11.57 Toroid.in :default @08:00:11.68 Toroid.seek :clear @08:00:13.60 Carousel.seek 16 @08:00:14.05 Toroid.in :hold @08:00:14.15 Toroid.seek :cartridge @08:00:16.02 Toroid.in :default @08:00:16.13 Toroid.seek :clear @08:00:17.97 delay 2 seconds @08:00:20.09 Toroid.in :hold @08:00:20.19 Toroid.seek :bypass @08:00:21.87 Toroid.in :default @08:00:21.97 Toroid.seek :clear @08:00:23.90 Carousel.seek 17 @08:00:24.43 Toroid.in :hold @08:00:24.53 Toroid.seek :cartridge @08:00:26.42 Toroid.in :default @08:00:26.53 Toroid.seek :clear @08:00:28.41 delay 2 seconds @08:00:30.52 Toroid.in :hold @08:00:30.64 Toroid.seek :bypass @08:00:32.32 Toroid.in :default @08:00:32.47 Toroid.seek :clear @08:00:34.42 Carousel.seek 18 @08:00:34.87 Toroid.in :hold @08:00:34.98 Toroid.seek :cartridge @08:00:36.86 Toroid.in :default @08:00:36.97 Toroid.seek :clear @08:00:38.81 delay 2 seconds @08:00:40.93 Toroid.in :hold @08:00:41.60 Toroid.seek :bypass @08:00:43.38 Toroid.in :default @08:00:43.47 Toroid.seek :clear @08:00:45.40 Carousel.seek 19 @08:00:45.85 Toroid.in :hold @08:00:45.95 Toroid.seek :cartridge @08:00:47.81 Toroid.in :default @08:00:47.91 Toroid.seek :clear @08:00:49.76 delay 2 seconds @08:00:51.88 Toroid.in :hold @08:00:51.97 Toroid.seek :bypass @08:00:53.65 Toroid.in :default @08:00:53.76 Toroid.seek :clear @08:00:55.69 Carousel.seek 20 @08:00:56.15 Toroid.in :hold @08:00:56.25 Toroid.seek :cartridge @08:00:58.13 Toroid.in :default @08:00:58.23 Toroid.seek :clear @08:01:00.08 delay 2 seconds @08:01:02.19 Toroid.in :hold @08:01:02.29 Toroid.seek :bypass @08:01:04.01 Toroid.in :default @08:01:04.12 Toroid.seek :clear @08:01:06.04 Carousel.seek 21 @08:01:06.48 Toroid.in :hold @08:01:06.59 Toroid.seek :cartridge @08:01:08.47 Toroid.in :default @08:01:08.56 Toroid.seek :clear @08:01:10.41 delay 2 seconds @08:01:12.52 Toroid.in :hold @08:01:12.63 Toroid.seek :bypass @08:01:14.30 Toroid.in :default @08:01:14.44 Toroid.seek :clear @08:01:16.41 Carousel.seek 22 @08:01:16.86 Toroid.in :hold @08:01:16.96 Toroid.seek :cartridge @08:01:18.84 Toroid.in :default @08:01:18.93 Toroid.seek :clear @08:01:20.78 delay 2 seconds @08:01:22.89 Toroid.in :hold @08:01:23.00 Toroid.seek :bypass @08:01:25.24 Toroid.in :default @08:01:25.43 Toroid.seek :clear @08:01:27.36 Carousel.seek 23 @08:01:27.81 Toroid.in :hold @08:01:27.91 Toroid.seek :cartridge @08:01:29.79 Toroid.in :default @08:01:29.89 Toroid.seek :clear @08:01:31.73 delay 2 seconds @08:01:33.85 Toroid.in :hold @08:01:33.93 Toroid.seek :bypass @08:01:35.61 Toroid.in :default @08:01:35.71 Toroid.seek :clear @08:01:37.65 Carousel.seek 24 @08:01:38.10 Toroid.in :hold @08:01:38.21 Toroid.seek :cartridge @08:01:40.08 Toroid.in :default @08:01:40.18 Toroid.seek :clear @08:01:42.03 delay 2 seconds @08:01:44.15 Toroid.in :hold @08:01:44.26 Toroid.seek :bypass @08:01:45.93 Toroid.in :default @08:01:46.04 Toroid.seek :clear @08:01:47.96 Carousel.seek 25 @08:01:48.43 Toroid.in :hold @08:01:48.53 Toroid.seek :cartridge @08:01:50.44 Toroid.in :default @08:01:50.54 Toroid.seek :clear @08:01:52.41 delay 2 seconds @08:01:54.53 Toroid.in :hold @08:01:54.64 Toroid.seek :bypass @08:01:56.31 Toroid.in :default @08:01:56.46 Toroid.seek :clear @08:01:58.39 Carousel.seek 26 @08:01:58.83 Toroid.in :hold @08:01:58.94 Toroid.seek :cartridge @08:02:00.82 Toroid.in :default @08:02:00.92 Toroid.seek :clear @08:02:02.77 delay 2 seconds @08:02:04.88 Toroid.in :hold @08:02:04.98 Toroid.seek :bypass @08:02:06.66 Toroid.in :default @08:02:06.86 Toroid.seek :clear @08:02:08.80 Carousel.seek 27 @08:02:09.25 Toroid.in :hold @08:02:09.35 Toroid.seek :cartridge @08:02:11.23 Toroid.in :default @08:02:11.33 Toroid.seek :clear @08:02:13.18 delay 2 seconds @08:02:15.30 Toroid.in :hold @08:02:15.40 Toroid.seek :bypass @08:02:17.08 Toroid.in :default @08:02:17.19 Toroid.seek :clear @08:02:19.11 Carousel.seek 28 @08:02:19.56 Toroid.in :hold @08:02:19.66 Toroid.seek :cartridge @08:02:21.54 Toroid.in :default @08:02:21.64 Toroid.seek :clear @08:02:23.49 delay 2 seconds @08:02:25.61 Toroid.in :hold @08:02:25.71 Toroid.seek :bypass @08:02:27.38 Toroid.in :default @08:02:27.47 Toroid.seek :clear @08:02:29.39 Carousel.seek 29 @08:02:29.85 Toroid.in :hold @08:02:29.94 Toroid.seek :cartridge @08:02:31.82 Toroid.in :default @08:02:31.92 Toroid.seek :clear @08:02:33.76 delay 2 seconds @08:02:35.88 Toroid.in :hold @08:02:35.99 Toroid.seek :bypass @08:02:37.66 Toroid.in :default @08:02:37.76 Toroid.seek :clear @08:02:39.68 Carousel.seek 30 @08:02:40.12 Toroid.in :hold @08:02:40.24 Toroid.seek :cartridge @08:02:42.11 Toroid.in :default @08:02:42.20 Toroid.seek :clear @08:02:44.05 delay 2 seconds @08:02:46.16 Toroid.in :hold @08:02:46.27 Toroid.seek :bypass @08:02:47.97 Toroid.in :default @08:02:48.63 Toroid.seek :clear @08:02:50.71 Carousel.seek 31 @08:02:51.16 Toroid.in :hold @08:02:51.26 Toroid.seek :cartridge @08:02:53.15 Toroid.in :default @08:02:53.26 Toroid.seek :clear @08:02:55.11 delay 2 seconds @08:02:57.22 Toroid.in :hold @08:02:57.33 Toroid.seek :bypass @08:02:59.01 Toroid.in :default @08:02:59.12 Toroid.seek :clear @08:03:01.04 Carousel.seek 32 @08:03:01.50 Toroid.in :hold @08:03:01.60 Toroid.seek :cartridge @08:03:03.47 Toroid.in :default @08:03:03.57 Toroid.seek :clear @08:03:05.42 delay 2 seconds @08:03:07.53 Toroid.in :hold @08:03:07.62 Toroid.seek :bypass @08:03:09.31 Toroid.in :default @08:03:09.42 Toroid.seek :clear @08:03:11.38 Carousel.seek 33 @08:03:11.84 Toroid.in :hold @08:03:11.94 Toroid.seek :cartridge @08:03:13.83 Toroid.in :default @08:03:13.96 Toroid.seek :clear @08:03:14.37 -> slots {33 => { Type: :unknown, State: :dry, TV: :open}, [60..34,32..1] => { Type: :unknown, State: :dry}} @08:03:15.81 delay 2 seconds @08:03:17.93 Toroid.in :hold @08:03:18.03 Toroid.seek :bypass @08:03:19.70 Toroid.in :default @08:03:19.81 Toroid.seek :clear @08:03:21.73 Carousel.seek 34 @08:03:22.18 Toroid.in :hold @08:03:22.28 Toroid.seek :cartridge @08:03:24.20 Toroid.in :default @08:03:24.30 Toroid.seek :clear @08:03:26.16 delay 2 seconds @08:03:28.29 Toroid.in :hold @08:03:28.44 Toroid.seek :bypass @08:03:30.12 Toroid.in :default @08:03:30.89 Toroid.seek :clear @08:03:32.83 Carousel.seek 35 @08:03:33.27 Toroid.in :hold @08:03:33.37 Toroid.seek :cartridge @08:03:33.53 -> slots 1..60=>:dry {35 => { Type: :unknown, State: :dry, TV: :"?"}, [60..36,34..1] => { Type: :unknown, State: :dry}} @08:03:35.33 Toroid.in :default @08:03:35.44 Toroid.seek :clear @08:03:37.29 delay 2 seconds @08:03:39.41 Toroid.in :hold @08:03:39.51 Toroid.seek :bypass @08:03:41.19 Toroid.in :default @08:03:41.30 Toroid.seek :clear @08:03:41.49 -> slots {60..1 => { Type: :unknown, State: :dry}} @08:03:43.24 Carousel.seek 36 @08:03:43.70 Toroid.in :hold @08:03:43.81 Toroid.seek :cartridge @08:03:45.69 Toroid.in :default @08:03:45.80 Toroid.seek :clear @08:03:47.65 delay 2 seconds @08:03:49.76 Toroid.in :hold @08:03:49.86 Toroid.seek :bypass @08:03:51.52 Toroid.in :default @08:03:51.63 Toroid.seek :clear @08:03:53.55 Carousel.seek 37 @08:03:53.99 Toroid.in :hold @08:03:54.09 Toroid.seek :cartridge @08:03:55.97 Toroid.in :default @08:03:56.07 Toroid.seek :clear @08:03:57.90 delay 2 seconds @08:04:00.02 Toroid.in :hold @08:04:00.12 Toroid.seek :bypass @08:04:01.80 Toroid.in :default @08:04:01.90 Toroid.seek :clear @08:04:03.82 Carousel.seek 38 @08:04:04.29 Toroid.in :hold @08:04:04.43 Toroid.seek :cartridge @08:04:06.30 Toroid.in :default @08:04:06.43 Toroid.seek :clear @08:04:08.28 delay 2 seconds @08:04:10.42 Toroid.in :hold @08:04:10.53 Toroid.seek :bypass @08:04:12.77 Toroid.in :default @08:04:12.97 Toroid.seek :clear @08:04:14.92 Carousel.seek 39 @08:04:15.36 Toroid.in :hold @08:04:15.47 Toroid.seek :cartridge @08:04:17.34 Toroid.in :default @08:04:17.43 Toroid.seek :clear @08:04:19.28 delay 2 seconds @08:04:21.40 Toroid.in :hold @08:04:21.51 Toroid.seek :bypass @08:04:23.20 Toroid.in :default @08:04:23.31 Toroid.seek :clear @08:04:25.25 Carousel.seek 40 @08:04:25.70 Toroid.in :hold @08:04:25.85 Toroid.seek :cartridge @08:04:27.73 Toroid.in :default @08:04:27.83 Toroid.seek :clear @08:04:29.65 delay 2 seconds @08:04:31.77 Toroid.in :hold @08:04:31.87 Toroid.seek :bypass @08:04:33.54 Toroid.in :default @08:04:33.64 Toroid.seek :clear @08:04:35.57 Carousel.seek 41 @08:04:35.68 -> slots[55..52]=>nil @08:04:35.73 SyntaxError in n -- compile error n:14: syntax error, unexpected tASSOC, expecting $end slots[55..52]=>nil ^ @08:04:36.04 Toroid.in :hold @08:04:36.15 Toroid.seek :cartridge @08:04:38.03 Toroid.in :default @08:04:38.13 Toroid.seek :clear @08:04:39.97 delay 2 seconds @08:04:42.09 Toroid.in :hold @08:04:42.19 Toroid.seek :bypass @08:04:43.87 Toroid.in :default @08:04:43.98 Toroid.seek :clear @08:04:45.92 Carousel.seek 42 @08:04:46.42 Toroid.in :hold @08:04:46.53 Toroid.seek :cartridge @08:04:48.43 Toroid.in :default @08:04:48.53 Toroid.seek :clear @08:04:50.41 delay 2 seconds @08:04:51.34 -> slots [55..52]=>nil {55..52 => nil} @08:04:52.52 Toroid.in :hold @08:04:52.62 Toroid.seek :bypass @08:04:54.94 Toroid.in :default @08:04:55.05 -> slots @08:04:55.08 Toroid.seek :clear {[60..56,51..1] => { Type: :unknown, State: :dry}} @08:04:57.13 Carousel.seek 43 @08:04:57.60 Toroid.in :hold @08:04:57.69 Toroid.seek :cartridge @08:04:59.57 Toroid.in :default @08:04:59.68 Toroid.seek :clear @08:05:01.53 delay 2 seconds @08:05:03.64 Toroid.in :hold @08:05:03.74 Toroid.seek :bypass @08:05:05.42 Toroid.in :default @08:05:05.52 Toroid.seek :clear @08:05:07.44 Carousel.seek 44 @08:05:07.89 Toroid.in :hold @08:05:08.00 Toroid.seek :cartridge @08:05:09.87 Toroid.in :default @08:05:09.97 Toroid.seek :clear @08:05:11.82 delay 2 seconds @08:05:13.93 Toroid.in :hold @08:05:14.04 Toroid.seek :bypass @08:05:15.72 Toroid.in :default @08:05:15.82 Toroid.seek :clear @08:05:17.75 Carousel.seek 45 @08:05:18.19 Toroid.in :hold @08:05:18.30 Toroid.seek :cartridge @08:05:20.21 Toroid.in :default @08:05:20.31 Toroid.seek :clear @08:05:22.19 delay 2 seconds @08:05:24.30 Toroid.in :hold @08:05:24.44 Toroid.seek :bypass @08:05:26.11 Toroid.in :default @08:05:26.21 Toroid.seek :clear @08:05:28.14 Carousel.seek 46 @08:05:28.59 Toroid.in :hold @08:05:28.71 Toroid.seek :cartridge @08:05:30.58 Toroid.in :default @08:05:30.69 Toroid.seek :clear @08:05:32.53 delay 2 seconds @08:05:34.64 Toroid.in :hold @08:05:34.74 Toroid.seek :bypass @08:05:37.07 Toroid.in :default @08:05:37.17 Toroid.seek :clear @08:05:39.12 Carousel.seek 47 @08:05:39.57 Toroid.in :hold @08:05:39.67 Toroid.seek :cartridge @08:05:41.54 Toroid.in :default @08:05:41.64 Toroid.seek :clear @08:05:43.49 delay 2 seconds @08:05:45.60 Toroid.in :hold @08:05:45.70 Toroid.seek :bypass @08:05:47.38 Toroid.in :default @08:05:47.48 Toroid.seek :clear @08:05:49.41 Carousel.seek 48 @08:05:49.86 Toroid.in :hold @08:05:49.97 Toroid.seek :cartridge @08:05:51.83 Toroid.in :default @08:05:51.93 Toroid.seek :clear @08:05:53.78 delay 2 seconds @08:05:55.90 Toroid.in :hold @08:05:56.00 Toroid.seek :bypass @08:05:57.68 Toroid.in :default @08:05:57.78 Toroid.seek :clear @08:05:59.72 Carousel.seek 49 @08:06:00.18 Toroid.in :hold @08:06:00.28 Toroid.seek :cartridge @08:06:02.18 Toroid.in :default @08:06:02.28 Toroid.seek :clear @08:06:04.15 delay 2 seconds @08:06:06.27 Toroid.in :hold @08:06:06.41 Toroid.seek :bypass @08:06:08.08 Toroid.in :default @08:06:08.17 Toroid.seek :clear @08:06:10.11 Carousel.seek 50 @08:06:10.53 Toroid.in :hold @08:06:10.64 Toroid.seek :cartridge @08:06:12.51 Toroid.in :default @08:06:12.62 Toroid.seek :clear @08:06:14.47 delay 2 seconds @08:06:16.58 Toroid.in :hold @08:06:16.69 Toroid.seek :bypass @08:06:18.93 Toroid.in :default @08:06:19.12 Toroid.seek :clear @08:06:21.06 Carousel.seek 51 @08:06:21.52 Toroid.in :hold @08:06:21.62 Toroid.seek :cartridge @08:06:23.49 Toroid.in :default @08:06:23.59 Toroid.seek :clear @08:06:25.44 delay 2 seconds @08:06:27.56 Toroid.in :hold @08:06:27.65 Toroid.seek :bypass @08:06:29.32 Toroid.in :default @08:06:29.43 Toroid.seek :clear @08:06:31.37 Carousel.seek 52 @08:06:31.82 Toroid.in :hold @08:06:31.92 Toroid.seek :cartridge @08:06:33.80 Toroid.in :default @08:06:33.92 Toroid.seek :clear @08:06:35.78 delay 2 seconds @08:06:37.89 Toroid.in :hold @08:06:38.00 Toroid.seek :bypass @08:06:39.70 Toroid.in :default @08:06:39.80 Toroid.seek :clear @08:06:41.73 Carousel.seek 53 @08:06:42.18 Toroid.in :hold @08:06:42.27 Toroid.seek :cartridge @08:06:44.17 Toroid.in :default @08:06:44.28 Toroid.seek :clear @08:06:46.14 delay 2 seconds @08:06:48.25 Toroid.in :hold @08:06:48.39 Toroid.seek :bypass @08:06:50.07 Toroid.in :default @08:06:50.18 Toroid.seek :clear @08:06:52.11 Carousel.seek 54 @08:06:52.56 Toroid.in :hold @08:06:52.67 Toroid.seek :cartridge @08:06:54.54 Toroid.in :default @08:06:54.65 Toroid.seek :clear @08:06:56.49 delay 2 seconds @08:06:58.60 Toroid.in :hold @08:06:58.71 Toroid.seek :bypass @08:07:00.97 Toroid.in :default @08:07:01.17 Toroid.seek :clear @08:07:03.10 Carousel.seek 55 @08:07:03.56 Toroid.in :hold @08:07:03.66 Toroid.seek :cartridge @08:07:05.53 Toroid.in :default @08:07:05.62 Toroid.seek :clear @08:07:07.47 delay 2 seconds @08:07:09.59 Toroid.in :hold @08:07:09.69 Toroid.seek :bypass @08:07:11.37 Toroid.in :default @08:07:11.49 Toroid.seek :clear @08:07:13.41 Carousel.seek 56 @08:07:13.86 Toroid.in :hold @08:07:13.96 Toroid.seek :cartridge @08:07:15.84 Toroid.in :default @08:07:15.95 Toroid.seek :clear @08:07:17.79 delay 2 seconds @08:07:19.91 Toroid.in :hold @08:07:20.02 Toroid.seek :bypass @08:07:21.70 Toroid.in :default @08:07:21.81 Toroid.seek :clear @08:07:23.73 Carousel.seek 57 @08:07:24.18 Toroid.in :hold @08:07:24.28 Toroid.seek :cartridge @08:07:26.18 Toroid.in :default @08:07:26.29 Toroid.seek :clear @08:07:28.17 delay 2 seconds @08:07:30.28 Toroid.in :hold @08:07:30.42 Toroid.seek :bypass @08:07:32.08 Toroid.in :default @08:07:32.19 Toroid.seek :clear @08:07:34.11 Carousel.seek 58 @08:07:34.56 Toroid.in :hold @08:07:34.67 Toroid.seek :cartridge @08:07:36.54 Toroid.in :default @08:07:36.65 Toroid.seek :clear @08:07:38.49 delay 2 seconds @08:07:40.60 Toroid.in :hold @08:07:40.70 Toroid.seek :bypass @08:07:42.42 Toroid.in :default @08:07:43.08 Toroid.seek :clear @08:07:45.11 Carousel.seek 59 @08:07:45.56 Toroid.in :hold @08:07:45.67 Toroid.seek :cartridge @08:07:47.55 Toroid.in :default @08:07:47.65 Toroid.seek :clear @08:07:49.50 delay 2 seconds @08:07:51.61 Toroid.in :hold @08:07:51.71 Toroid.seek :bypass @08:07:53.39 Toroid.in :default @08:07:53.50 Toroid.seek :clear @08:07:55.40 Carousel.seek 60 @08:07:55.85 Toroid.in :hold @08:07:55.96 Toroid.seek :cartridge @08:07:57.84 Toroid.in :default @08:07:57.93 Toroid.seek :clear @08:07:59.76 delay 2 seconds @08:08:01.88 Toroid.in :hold @08:08:01.98 Toroid.seek :bypass @08:08:03.66 Toroid.in :default @08:08:03.75 Toroid.seek :clear @08:08:05.41 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @08:08:51.28 UserAbort in bill -- User Abort!! @08:09:07.12 -> vacToroid @08:09:07.20 Vacuuming protocol for dry cartridges @08:09:08.02 Toroid.seek :clear @08:09:08.24 Carousel.in :default @08:09:08.43 Carousel.home.seek 60 @08:09:08.72 Waiting for bill to answer "Disconnect the intake line. push return" @08:09:13.47 Answered Disconnect the intake line. push return"" @08:09:14.32 Toroid.seek :clear @08:09:14.57 Carousel.in :default @08:09:14.75 Carousel.home.seek 60 @08:09:15.05 SamplePump.setPosition! 0ml @08:09:15.13 Intake.open @08:09:15.87 SamplePump.seek 50ml @08:09:51.12 -> slots {55..52 => { Type: :unknown, State: :missing}, [60..56,51..1] => { Type: :unknown, State: :dry}} @08:10:17.49 Exhaust.close @08:10:18.79 SamplePump.seek 75ml @08:10:49.91 Intake.close @08:10:50.60 Bypass.close @08:10:51.33 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @08:10:51.40 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @08:10:51.42 Exhaust.open @08:10:52.11 Bypass.open @08:10:52.82 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @08:11:38.17 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @08:11:38.19 delay 30 seconds @08:12:08.25 Clearing fluid from cartridge-side of Toroid @08:12:08.63 Carousel.seek 1 @08:12:18.32 -> slots {55..52 => { Type: :unknown, State: :missing}, [60..56,51..1] => { Type: :unknown, State: :dry}} @08:12:20.90 Toroid.in :hold @08:12:21.00 Toroid.seek :cartridge @08:12:22.89 Toroid.in :default @08:12:23.00 Toroid.seek :clear @08:12:24.84 delay 2 seconds @08:12:26.95 Toroid.in :hold @08:12:27.05 Toroid.seek :bypass @08:12:28.72 Toroid.in :default @08:12:28.81 Toroid.seek :clear @08:12:30.75 Carousel.seek 2 @08:12:31.21 Toroid.in :hold @08:12:31.33 Toroid.seek :cartridge @08:12:33.24 Toroid.in :default @08:12:33.34 Toroid.seek :clear @08:12:35.22 delay 2 seconds @08:12:37.34 Toroid.in :hold @08:12:37.44 Toroid.seek :bypass @08:12:39.12 Toroid.in :default @08:12:39.22 Toroid.seek :clear @08:12:41.14 Carousel.seek 3 @08:12:41.60 Toroid.in :hold @08:12:41.71 Toroid.seek :cartridge @08:12:43.58 Toroid.in :default @08:12:43.68 Toroid.seek :clear @08:12:45.53 delay 2 seconds @08:12:47.65 Toroid.in :hold @08:12:47.75 Toroid.seek :bypass @08:12:49.43 Toroid.in :default @08:12:49.54 Toroid.seek :clear @08:12:50.05 -> Cartridge.reserve 55..52 0 @08:12:51.48 Carousel.seek 4 @08:12:51.93 Toroid.in :hold @08:12:52.04 Toroid.seek :cartridge @08:12:52.98 -> slots {4 => { Type: :unknown, State: :dry, TV: :"?"}, 55..52 => { Type: :unknown, State: :missing}, [60..56,51..5,3..1] => { Type: :unknown, State: :dry}} @08:12:53.91 Toroid.in :default @08:12:54.02 Toroid.seek :clear @08:12:55.86 delay 2 seconds @08:12:57.97 Toroid.in :hold @08:12:58.07 Toroid.seek :bypass @08:12:59.75 Toroid.in :default @08:12:59.84 Toroid.seek :clear @08:13:01.77 Carousel.seek 5 @08:13:02.22 Toroid.in :hold @08:13:02.33 Toroid.seek :cartridge @08:13:04.25 Toroid.in :default @08:13:04.36 Toroid.seek :clear @08:13:06.23 delay 2 seconds @08:13:08.37 Toroid.in :hold @08:13:08.50 Toroid.seek :bypass @08:13:10.18 Toroid.in :default @08:13:10.29 Toroid.seek :clear @08:13:12.23 Carousel.seek 6 @08:13:12.68 Toroid.in :hold @08:13:12.79 Toroid.seek :cartridge @08:13:14.67 Toroid.in :default @08:13:14.77 Toroid.seek :clear @08:13:16.62 delay 2 seconds @08:13:18.74 Toroid.in :hold @08:13:18.84 Toroid.seek :bypass @08:13:20.51 Toroid.in :default @08:13:20.61 Toroid.seek :clear @08:13:22.57 Carousel.seek 7 @08:13:23.02 Toroid.in :hold @08:13:23.13 Toroid.seek :cartridge @08:13:25.01 Toroid.in :default @08:13:25.10 Toroid.seek :clear @08:13:26.95 delay 2 seconds @08:13:29.07 Toroid.in :hold @08:13:29.17 Toroid.seek :bypass @08:13:30.84 Toroid.in :default @08:13:30.95 Toroid.seek :clear @08:13:32.87 Carousel.seek 8 @08:13:33.29 Toroid.in :hold @08:13:33.39 Toroid.seek :cartridge @08:13:35.83 Toroid.in :default @08:13:36.03 Toroid.seek :clear @08:13:37.85 delay 2 seconds @08:13:39.97 Toroid.in :hold @08:13:40.08 Toroid.seek :bypass @08:13:41.75 Toroid.in :default @08:13:41.86 Toroid.seek :clear @08:13:43.80 Carousel.seek 9 @08:13:44.25 Toroid.in :hold @08:13:44.38 Toroid.seek :cartridge @08:13:46.26 Toroid.in :default @08:13:46.41 Toroid.seek :clear @08:13:48.26 delay 2 seconds @08:13:50.41 Toroid.in :hold @08:13:50.51 Toroid.seek :bypass @08:13:52.19 Toroid.in :default @08:13:52.29 Toroid.seek :clear @08:13:54.27 Carousel.seek 10 @08:13:54.72 Toroid.in :hold @08:13:54.83 Toroid.seek :cartridge @08:13:56.70 Toroid.in :default @08:13:56.80 Toroid.seek :clear @08:13:58.65 delay 2 seconds @08:14:00.76 Toroid.in :hold @08:14:00.87 Toroid.seek :bypass @08:14:02.55 Toroid.in :default @08:14:02.68 Toroid.seek :clear @08:14:04.64 Carousel.seek 11 @08:14:05.09 Toroid.in :hold @08:14:05.20 Toroid.seek :cartridge @08:14:07.07 Toroid.in :default @08:14:07.16 Toroid.seek :clear @08:14:09.02 delay 2 seconds @08:14:11.13 Toroid.in :hold @08:14:11.23 Toroid.seek :bypass @08:14:12.91 Toroid.in :default @08:14:13.01 Toroid.seek :clear @08:14:14.93 Carousel.seek 12 @08:14:15.38 Toroid.in :hold @08:14:15.49 Toroid.seek :cartridge @08:14:17.90 Toroid.in :default @08:14:18.09 Toroid.seek :clear @08:14:19.94 delay 2 seconds @08:14:22.05 Toroid.in :hold @08:14:22.15 Toroid.seek :bypass @08:14:23.82 Toroid.in :default @08:14:23.92 Toroid.seek :clear @08:14:25.86 Carousel.seek 13 @08:14:26.32 Toroid.in :hold @08:14:26.46 Toroid.seek :cartridge @08:14:28.33 Toroid.in :default @08:14:28.47 Toroid.seek :clear @08:14:30.31 delay 2 seconds @08:14:32.47 Toroid.in :hold @08:14:32.58 Toroid.seek :bypass @08:14:34.26 Toroid.in :default @08:14:34.38 Toroid.seek :clear @08:14:36.32 Carousel.seek 14 @08:14:36.80 Toroid.in :hold @08:14:36.89 Toroid.seek :cartridge @08:14:38.77 Toroid.in :default @08:14:38.87 Toroid.seek :clear @08:14:40.72 delay 2 seconds @08:14:42.84 Toroid.in :hold @08:14:42.94 Toroid.seek :bypass @08:14:44.62 Toroid.in :default @08:14:44.72 Toroid.seek :clear @08:14:46.69 Carousel.seek 15 @08:14:47.14 Toroid.in :hold @08:14:47.24 Toroid.seek :cartridge @08:14:49.12 Toroid.in :default @08:14:49.22 Toroid.seek :clear @08:14:51.07 delay 2 seconds @08:14:53.18 Toroid.in :hold @08:14:53.34 Toroid.seek :bypass @08:14:55.02 Toroid.in :default @08:14:55.12 Toroid.seek :clear @08:14:57.03 Carousel.seek 16 @08:14:57.48 Toroid.in :hold @08:14:57.58 Toroid.seek :cartridge @08:14:59.46 Toroid.in :default @08:15:00.10 Toroid.seek :clear @08:15:02.06 delay 2 seconds @08:15:04.18 Toroid.in :hold @08:15:04.28 Toroid.seek :bypass @08:15:05.96 Toroid.in :default @08:15:06.06 Toroid.seek :clear @08:15:07.99 Carousel.seek 17 @08:15:08.44 Toroid.in :hold @08:15:08.55 Toroid.seek :cartridge @08:15:10.44 Toroid.in :default @08:15:10.54 Toroid.seek :clear @08:15:12.42 delay 2 seconds @08:15:14.54 Toroid.in :hold @08:15:14.64 Toroid.seek :bypass @08:15:16.33 Toroid.in :default @08:15:16.48 Toroid.seek :clear @08:15:18.44 Carousel.seek 18 @08:15:18.89 Toroid.in :hold @08:15:18.99 Toroid.seek :cartridge @08:15:20.87 Toroid.in :default @08:15:20.97 Toroid.seek :clear @08:15:22.83 delay 2 seconds @08:15:24.94 Toroid.in :hold @08:15:25.04 Toroid.seek :bypass @08:15:26.74 Toroid.in :default @08:15:26.85 Toroid.seek :clear @08:15:28.77 Carousel.seek 19 @08:15:29.22 Toroid.in :hold @08:15:29.32 Toroid.seek :cartridge @08:15:31.19 Toroid.in :default @08:15:31.30 Toroid.seek :clear @08:15:33.12 delay 2 seconds @08:15:35.23 Toroid.in :hold @08:15:35.34 Toroid.seek :bypass @08:15:37.02 Toroid.in :default @08:15:37.13 Toroid.seek :clear @08:15:39.08 Carousel.seek 20 @08:15:39.54 Toroid.in :hold @08:15:39.65 Toroid.seek :cartridge @08:15:41.52 Toroid.in :default @08:15:41.62 Toroid.seek :clear @08:15:44.02 delay 2 seconds @08:15:46.23 Toroid.in :hold @08:15:46.34 Toroid.seek :bypass @08:15:48.07 Toroid.in :default @08:15:48.17 Toroid.seek :clear @08:15:50.11 Carousel.seek 21 @08:15:50.56 Toroid.in :hold @08:15:50.68 Toroid.seek :cartridge @08:15:52.55 Toroid.in :default @08:15:52.66 Toroid.seek :clear @08:15:54.50 delay 2 seconds @08:15:56.62 Toroid.in :hold @08:15:56.72 Toroid.seek :bypass @08:15:58.42 Toroid.in :default @08:15:58.54 Toroid.seek :clear @08:16:00.49 Carousel.seek 22 @08:16:00.94 Toroid.in :hold @08:16:01.04 Toroid.seek :cartridge @08:16:02.92 Toroid.in :default @08:16:03.02 Toroid.seek :clear @08:16:04.86 delay 2 seconds @08:16:06.97 Toroid.in :hold @08:16:07.08 Toroid.seek :bypass @08:16:08.75 Toroid.in :default @08:16:08.86 Toroid.seek :clear @08:16:10.80 Carousel.seek 23 @08:16:11.25 Toroid.in :hold @08:16:11.36 Toroid.seek :cartridge @08:16:13.23 Toroid.in :default @08:16:13.33 Toroid.seek :clear @08:16:15.18 delay 2 seconds @08:16:17.29 Toroid.in :hold @08:16:17.39 Toroid.seek :bypass @08:16:19.05 Toroid.in :default @08:16:19.16 Toroid.seek :clear @08:16:21.08 Carousel.seek 24 @08:16:21.53 Toroid.in :hold @08:16:21.62 Toroid.seek :cartridge @08:16:23.50 Toroid.in :default @08:16:23.61 Toroid.seek :clear @08:16:26.00 delay 2 seconds @08:16:28.22 Toroid.in :hold @08:16:28.32 Toroid.seek :bypass @08:16:30.05 Toroid.in :default @08:16:30.15 Toroid.seek :clear @08:16:32.09 Carousel.seek 25 @08:16:32.54 Toroid.in :hold @08:16:32.65 Toroid.seek :cartridge @08:16:34.51 Toroid.in :default @08:16:34.62 Toroid.seek :clear @08:16:36.46 delay 2 seconds @08:16:38.58 Toroid.in :hold @08:16:38.70 Toroid.seek :bypass @08:16:40.39 Toroid.in :default @08:16:40.50 Toroid.seek :clear @08:16:42.45 Carousel.seek 26 @08:16:42.89 Toroid.in :hold @08:16:42.99 Toroid.seek :cartridge @08:16:44.87 Toroid.in :default @08:16:44.97 Toroid.seek :clear @08:16:46.82 delay 2 seconds @08:16:48.95 Toroid.in :hold @08:16:49.05 Toroid.seek :bypass @08:16:50.73 Toroid.in :default @08:16:50.84 Toroid.seek :clear @08:16:52.75 Carousel.seek 27 @08:16:53.21 Toroid.in :hold @08:16:53.31 Toroid.seek :cartridge @08:16:55.18 Toroid.in :default @08:16:55.28 Toroid.seek :clear @08:16:57.13 delay 2 seconds @08:16:59.25 Toroid.in :hold @08:16:59.36 Toroid.seek :bypass @08:17:01.04 Toroid.in :default @08:17:01.15 Toroid.seek :clear @08:17:03.07 Carousel.seek 28 @08:17:03.51 Toroid.in :hold @08:17:03.61 Toroid.seek :cartridge @08:17:05.48 Toroid.in :default @08:17:05.58 Toroid.seek :clear @08:17:07.43 delay 2 seconds @08:17:10.09 Toroid.in :hold @08:17:10.28 Toroid.seek :bypass @08:17:11.99 Toroid.in :default @08:17:12.09 Toroid.seek :clear @08:17:14.03 Carousel.seek 29 @08:17:14.48 Toroid.in :hold @08:17:14.59 Toroid.seek :cartridge @08:17:16.45 Toroid.in :default @08:17:16.56 Toroid.seek :clear @08:17:18.41 delay 2 seconds @08:17:20.52 Toroid.in :hold @08:17:20.65 Toroid.seek :bypass @08:17:22.33 Toroid.in :default @08:17:22.47 Toroid.seek :clear @08:17:24.41 Carousel.seek 30 @08:17:24.86 Toroid.in :hold @08:17:24.97 Toroid.seek :cartridge @08:17:26.85 Toroid.in :default @08:17:26.95 Toroid.seek :clear @08:17:28.80 delay 2 seconds @08:17:30.91 Toroid.in :hold @08:17:31.01 Toroid.seek :bypass @08:17:32.69 Toroid.in :default @08:17:32.80 Toroid.seek :clear @08:17:34.75 Carousel.seek 31 @08:17:35.21 Toroid.in :hold @08:17:35.31 Toroid.seek :cartridge @08:17:37.19 Toroid.in :default @08:17:37.29 Toroid.seek :clear @08:17:39.13 delay 2 seconds @08:17:41.25 Toroid.in :hold @08:17:41.35 Toroid.seek :bypass @08:17:43.03 Toroid.in :default @08:17:43.13 Toroid.seek :clear @08:17:45.05 Carousel.seek 32 @08:17:45.46 Toroid.in :hold @08:17:45.57 Toroid.seek :cartridge @08:17:47.44 Toroid.in :default @08:17:47.54 Toroid.seek :clear @08:17:49.38 delay 2 seconds @08:17:51.49 Toroid.in :hold @08:17:52.13 Toroid.seek :bypass @08:17:53.92 Toroid.in :default @08:17:54.03 Toroid.seek :clear @08:17:55.96 Carousel.seek 33 @08:17:56.43 Toroid.in :hold @08:17:56.53 Toroid.seek :cartridge @08:17:58.43 Toroid.in :default @08:17:58.54 Toroid.seek :clear @08:18:00.42 delay 2 seconds @08:18:02.53 Toroid.in :hold @08:18:02.65 Toroid.seek :bypass @08:18:04.32 Toroid.in :default @08:18:04.47 Toroid.seek :clear @08:18:06.44 Carousel.seek 34 @08:18:06.89 Toroid.in :hold @08:18:06.99 Toroid.seek :cartridge @08:18:08.87 Toroid.in :default @08:18:08.98 Toroid.seek :clear @08:18:10.83 delay 2 seconds @08:18:12.94 Toroid.in :hold @08:18:13.05 Toroid.seek :bypass @08:18:14.73 Toroid.in :default @08:18:14.83 Toroid.seek :clear @08:18:16.77 Carousel.seek 35 @08:18:17.22 Toroid.in :hold @08:18:17.31 Toroid.seek :cartridge @08:18:19.19 Toroid.in :default @08:18:19.29 Toroid.seek :clear @08:18:21.14 delay 2 seconds @08:18:23.26 Toroid.in :hold @08:18:23.36 Toroid.seek :bypass @08:18:25.03 Toroid.in :default @08:18:25.14 Toroid.seek :clear @08:18:27.06 Carousel.seek 36 @08:18:27.50 Toroid.in :hold @08:18:27.61 Toroid.seek :cartridge @08:18:29.48 Toroid.in :default @08:18:29.57 Toroid.seek :clear @08:18:31.43 delay 2 seconds @08:18:33.54 Toroid.in :hold @08:18:33.65 Toroid.seek :bypass @08:18:36.01 Toroid.in :default @08:18:36.11 Toroid.seek :clear @08:18:38.05 Carousel.seek 37 @08:18:38.50 Toroid.in :hold @08:18:38.61 Toroid.seek :cartridge @08:18:40.48 Toroid.in :default @08:18:40.60 Toroid.seek :clear @08:18:42.43 delay 2 seconds @08:18:44.56 Toroid.in :hold @08:18:44.67 Toroid.seek :bypass @08:18:46.35 Toroid.in :default @08:18:46.48 Toroid.seek :clear @08:18:48.44 Carousel.seek 38 @08:18:48.88 Toroid.in :hold @08:18:48.99 Toroid.seek :cartridge @08:18:50.86 Toroid.in :default @08:18:50.97 Toroid.seek :clear @08:18:52.81 delay 2 seconds @08:18:54.92 Toroid.in :hold @08:18:55.03 Toroid.seek :bypass @08:18:56.70 Toroid.in :default @08:18:56.80 Toroid.seek :clear @08:18:58.72 Carousel.seek 39 @08:18:59.17 Toroid.in :hold @08:18:59.27 Toroid.seek :cartridge @08:19:01.15 Toroid.in :default @08:19:01.26 Toroid.seek :clear @08:19:03.10 delay 2 seconds @08:19:05.21 Toroid.in :hold @08:19:05.31 Toroid.seek :bypass @08:19:06.99 Toroid.in :default @08:19:07.10 Toroid.seek :clear @08:19:09.01 Carousel.seek 40 @08:19:09.46 Toroid.in :hold @08:19:09.57 Toroid.seek :cartridge @08:19:11.45 Toroid.in :default @08:19:11.54 Toroid.seek :clear @08:19:13.39 delay 2 seconds @08:19:15.50 Toroid.in :hold @08:19:15.60 Toroid.seek :bypass @08:19:17.28 Toroid.in :default @08:19:17.48 Toroid.seek :clear @08:19:19.41 Carousel.seek 41 @08:19:19.87 Toroid.in :hold @08:19:19.98 Toroid.seek :cartridge @08:19:21.85 Toroid.in :default @08:19:21.95 Toroid.seek :clear @08:19:23.80 delay 2 seconds @08:19:25.91 Toroid.in :hold @08:19:26.00 Toroid.seek :bypass @08:19:27.68 Toroid.in :default @08:19:27.78 Toroid.seek :clear @08:19:29.71 Carousel.seek 42 @08:19:30.15 Toroid.in :hold @08:19:30.25 Toroid.seek :cartridge @08:19:32.13 Toroid.in :default @08:19:32.22 Toroid.seek :clear @08:19:34.07 delay 2 seconds @08:19:36.18 Toroid.in :hold @08:19:36.29 Toroid.seek :bypass @08:19:38.00 Toroid.in :default @08:19:38.11 Toroid.seek :clear @08:19:40.03 Carousel.seek 43 @08:19:40.47 Toroid.in :hold @08:19:40.56 Toroid.seek :cartridge @08:19:42.44 Toroid.in :default @08:19:42.55 Toroid.seek :clear @08:19:44.41 delay 2 seconds @08:19:46.52 Toroid.in :hold @08:19:46.63 Toroid.seek :bypass @08:19:48.30 Toroid.in :default @08:19:48.42 Toroid.seek :clear @08:19:50.40 Carousel.seek 44 @08:19:50.86 Toroid.in :hold @08:19:50.97 Toroid.seek :cartridge @08:19:52.84 Toroid.in :default @08:19:52.95 Toroid.seek :clear @08:19:54.79 delay 2 seconds @08:19:56.90 Toroid.in :hold @08:19:57.01 Toroid.seek :bypass @08:19:58.67 Toroid.in :default @08:19:59.41 Toroid.seek :clear @08:20:01.34 Carousel.seek 45 @08:20:01.80 Toroid.in :hold @08:20:01.92 Toroid.seek :cartridge @08:20:03.79 Toroid.in :default @08:20:03.90 Toroid.seek :clear @08:20:05.74 delay 2 seconds @08:20:07.85 Toroid.in :hold @08:20:07.96 Toroid.seek :bypass @08:20:09.65 Toroid.in :default @08:20:09.74 Toroid.seek :clear @08:20:11.68 Carousel.seek 46 @08:20:12.13 Toroid.in :hold @08:20:12.24 Toroid.seek :cartridge @08:20:14.11 Toroid.in :default @08:20:14.22 Toroid.seek :clear @08:20:16.06 delay 2 seconds @08:20:18.18 Toroid.in :hold @08:20:18.28 Toroid.seek :bypass @08:20:19.96 Toroid.in :default @08:20:20.06 Toroid.seek :clear @08:20:22.00 Carousel.seek 47 @08:20:22.45 Toroid.in :hold @08:20:22.56 Toroid.seek :cartridge @08:20:24.43 Toroid.in :default @08:20:24.54 Toroid.seek :clear @08:20:26.41 delay 2 seconds @08:20:28.53 Toroid.in :hold @08:20:28.63 Toroid.seek :bypass @08:20:30.30 Toroid.in :default @08:20:30.43 Toroid.seek :clear @08:20:32.39 Carousel.seek 48 @08:20:32.84 Toroid.in :hold @08:20:32.95 Toroid.seek :cartridge @08:20:34.82 Toroid.in :default @08:20:34.92 Toroid.seek :clear @08:20:36.76 delay 2 seconds @08:20:38.88 Toroid.in :hold @08:20:38.99 Toroid.seek :bypass @08:20:40.68 Toroid.in :default @08:20:41.33 Toroid.seek :clear @08:20:43.38 Carousel.seek 49 @08:20:43.84 Toroid.in :hold @08:20:43.95 Toroid.seek :cartridge @08:20:45.83 Toroid.in :default @08:20:45.93 Toroid.seek :clear @08:20:47.78 delay 2 seconds @08:20:49.89 Toroid.in :hold @08:20:50.00 Toroid.seek :bypass @08:20:51.68 Toroid.in :default @08:20:51.80 Toroid.seek :clear @08:20:53.75 Carousel.seek 50 @08:20:54.20 Toroid.in :hold @08:20:54.30 Toroid.seek :cartridge @08:20:56.22 Toroid.in :default @08:20:56.33 Toroid.seek :clear @08:20:58.22 delay 2 seconds @08:21:00.33 Toroid.in :hold @08:21:00.47 Toroid.seek :bypass @08:21:02.15 Toroid.in :default @08:21:02.25 Toroid.seek :clear @08:21:04.18 Carousel.seek 51 @08:21:04.63 Toroid.in :hold @08:21:04.73 Toroid.seek :cartridge @08:21:06.61 Toroid.in :default @08:21:06.72 Toroid.seek :clear @08:21:08.58 delay 2 seconds @08:21:10.69 Toroid.in :hold @08:21:10.80 Toroid.seek :bypass @08:21:12.47 Toroid.in :default @08:21:12.59 Toroid.seek :clear @08:21:14.56 Carousel.seek 56 @08:21:15.82 Toroid.in :hold @08:21:15.93 Toroid.seek :cartridge @08:21:17.79 Toroid.in :default @08:21:17.90 Toroid.seek :clear @08:21:19.74 delay 2 seconds @08:21:21.85 Toroid.in :hold @08:21:21.95 Toroid.seek :bypass @08:21:23.66 Toroid.in :default @08:21:23.77 Toroid.seek :clear @08:21:26.28 Carousel.seek 57 @08:21:26.87 Toroid.in :hold @08:21:26.97 Toroid.seek :cartridge @08:21:28.86 Toroid.in :default @08:21:28.96 Toroid.seek :clear @08:21:30.81 delay 2 seconds @08:21:32.95 Toroid.in :hold @08:21:33.06 Toroid.seek :bypass @08:21:34.75 Toroid.in :default @08:21:34.85 Toroid.seek :clear @08:21:36.78 Carousel.seek 58 @08:21:37.25 Toroid.in :hold @08:21:37.35 Toroid.seek :cartridge @08:21:39.24 Toroid.in :default @08:21:39.35 Toroid.seek :clear @08:21:41.24 delay 2 seconds @08:21:43.36 Toroid.in :hold @08:21:43.46 Toroid.seek :bypass @08:21:45.13 Toroid.in :default @08:21:45.24 Toroid.seek :clear @08:21:47.16 Carousel.seek 59 @08:21:47.61 Toroid.in :hold @08:21:47.72 Toroid.seek :cartridge @08:21:49.58 Toroid.in :default @08:21:49.68 Toroid.seek :clear @08:21:51.53 delay 2 seconds @08:21:53.66 Toroid.in :hold @08:21:53.77 Toroid.seek :bypass @08:21:55.44 Toroid.in :default @08:21:55.54 Toroid.seek :clear @08:21:57.46 Carousel.seek 60 @08:21:57.90 Toroid.in :hold @08:21:58.01 Toroid.seek :cartridge @08:21:59.88 Toroid.in :default @08:21:59.98 Toroid.seek :clear @08:22:01.83 delay 2 seconds @08:22:03.94 Toroid.in :hold @08:22:04.04 Toroid.seek :bypass @08:22:05.69 Toroid.in :default @08:22:05.80 Toroid.seek :clear @08:22:08.09 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @08:30:13.00 -> SC.to 43 @08:30:13.06 Carousel.seek 43 Storage Carousel (SC) at 43 @08:35:05.46 -> SC.to 24 @08:35:05.52 Carousel.seek 24 Storage Carousel (SC) at 24 @08:40:33.09 -> SC.to 8 @08:40:33.16 Carousel.seek 8 Storage Carousel (SC) at 8 @08:44:03.57 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @08:44:04.46 Toroid.seek :clear @08:44:04.67 Carousel.in :default @08:44:04.84 Carousel.home.seek 8 @08:44:05.14 Waiting for bill to answer "Turn the pump on. Push Return" @08:45:21.28 Answered Turn the pump on. Push Return"" @08:45:21.31 Vacuuming individual cartridges. @08:45:22.18 Toroid.seek :clear @08:45:22.43 Carousel.in :default @08:45:22.59 Carousel.home.seek 8 @08:45:23.18 Carousel.seek 1 @08:45:24.97 Lysis1.in :fast @08:45:25.08 Lysis1.seek :locked @08:45:27.75 Plunge.seek :locked @08:45:27.96 Air.in :fast @08:45:28.06 Air.seek :locked @08:45:29.08 Carousel.reconfigure :locked @08:45:29.28 Powering cartridge bus @08:45:31.96 Gate.setIR 1750,5000,0,6 @08:45:32.10 Gate.setIR 0,5000,0,6 @08:45:32.21 PuckHeater.configure :default #ambient 21.9C @08:45:32.32 Gate.power :heat,:ON,limit to 5000mA @08:45:33.03 Mounted lysis Cartridge board#Q05059 @08:45:33.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:45:33.18 Vacuuming Vent Top cartridge 1 for 30 seconds @08:45:33.21 Air.in :default @08:45:33.33 Air.seek :evac @08:45:47.93 Toroid.in :hold @08:45:48.66 Toroid.seek :cartridge @08:45:50.59 Toroid.in :default @08:45:50.70 Toroid.seek :clear @08:45:52.55 Polling Can every 2:00 @08:45:53.10 PuckHeater.configure :default #ambient 21.1C @08:45:53.20 PuckHeater.seek 41.1C,1:00 @08:46:15.89 PuckHeater.hold 3 seconds,41.1C @08:46:19.03 delayUntil "08:46:21.71" @08:46:21.82 Toroid.in :hold @08:46:21.93 Toroid.seek :bypass @08:46:23.60 Toroid.in :default @08:46:23.71 Toroid.seek :clear @08:46:25.37 delay 2 seconds @08:46:27.40 Air.seek :full @08:46:42.05 Gate.power :heat,:OFF @08:46:42.08 Gate.power :cartridge,:OFF @08:46:42.23 Carousel.in :default @08:46:42.42 Carousel.home.seek 1 @08:46:42.90 Air.in :unlocking @08:46:43.06 Air.seek :clear @08:46:44.11 Lysis1.in :unlocking @08:46:44.24 Lysis1.seek :clear @08:46:44.45 Plunge.in :unlocking @08:46:44.55 Plunge.seek :clear @08:46:45.69 Carousel.seek 2 @08:46:46.28 Lysis1.in :fast @08:46:46.41 Lysis1.seek :locked @08:46:49.07 Plunge.in :default @08:46:49.21 Plunge.seek :locked @08:46:49.38 Air.in :fast @08:46:49.49 Air.seek :locked @08:46:51.15 Carousel.reconfigure :locked @08:46:51.38 Powering cartridge bus @08:46:54.06 Gate.setIR 1750,5000,0,6 @08:46:54.19 Gate.setIR 0,5000,0,6 @08:46:54.30 PuckHeater.configure :default #ambient 21.1C @08:46:54.45 Gate.power :heat,:ON,limit to 5000mA @08:46:55.15 Mounted lysis Cartridge board#Q05058 @08:46:55.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:46:55.28 Vacuuming Vent Top cartridge 2 for 30 seconds @08:46:55.33 Air.in :default @08:46:55.44 Air.seek :evac @08:47:10.02 Toroid.in :hold @08:47:10.13 Toroid.seek :cartridge @08:47:12.00 Toroid.in :default @08:47:12.11 Toroid.seek :clear @08:47:13.95 Polling Can every 2:00 @08:47:14.51 PuckHeater.configure :default #ambient 21.2C @08:47:14.62 PuckHeater.seek 41.1C,1:00 @08:47:37.29 PuckHeater.hold 3 seconds,41.1C @08:47:40.52 delayUntil "08:47:43.15" @08:47:43.25 Toroid.in :hold @08:47:43.35 Toroid.seek :bypass @08:47:45.03 Toroid.in :default @08:47:45.13 Toroid.seek :clear @08:47:46.79 delay 2 seconds @08:47:48.82 Air.seek :full @08:48:03.47 Gate.power :heat,:OFF @08:48:03.50 Gate.power :cartridge,:OFF @08:48:03.64 Carousel.in :default @08:48:03.80 Carousel.home.seek 2 @08:48:04.26 Air.in :unlocking @08:48:04.45 Air.seek :clear @08:48:05.50 Lysis1.in :unlocking @08:48:06.21 Lysis1.seek :clear @08:48:06.50 Plunge.in :unlocking @08:48:06.61 Plunge.seek :clear @08:48:07.77 Carousel.seek 3 @08:48:08.42 Lysis1.in :fast @08:48:08.51 Lysis1.seek :locked @08:48:11.18 Plunge.in :default @08:48:11.32 Plunge.seek :locked @08:48:11.50 Air.in :fast @08:48:11.61 Air.seek :locked @08:48:12.62 Carousel.reconfigure :locked @08:48:12.82 Powering cartridge bus @08:48:15.49 Gate.setIR 1750,5000,0,6 @08:48:15.62 Gate.setIR 0,5000,0,6 @08:48:15.74 Gate.power :cartridge,:OFF @08:48:16.33 Mounted archive Cartridge @08:48:16.47 Vacuuming Solid Top cartridge 3 for 30 seconds @08:48:16.61 Toroid.in :hold @08:48:16.71 Toroid.seek :cartridge @08:48:18.58 Toroid.in :default @08:48:18.68 Toroid.seek :clear @08:48:20.52 delay 30 seconds @08:48:50.65 Toroid.in :hold @08:48:50.75 Toroid.seek :bypass @08:48:52.44 Toroid.in :default @08:48:52.54 Toroid.seek :clear @08:48:54.45 Carousel.in :default @08:48:54.61 Carousel.home.seek 3 @08:48:55.08 Air.in :unlocking @08:48:55.27 Air.seek :clear @08:48:56.33 Lysis1.in :unlocking @08:48:56.54 Lysis1.seek :clear @08:48:56.69 Plunge.in :unlocking @08:48:56.80 Plunge.seek :clear @08:48:57.95 Carousel.seek 4 @08:48:58.57 Lysis1.in :fast @08:48:58.66 Lysis1.seek :locked @08:49:01.34 Plunge.in :default @08:49:01.49 Plunge.seek :locked @08:49:02.20 Air.in :fast @08:49:02.43 Air.seek :locked @08:49:03.45 Carousel.reconfigure :locked @08:49:03.66 Powering cartridge bus @08:49:06.33 Gate.setIR 1750,5000,0,6 @08:49:06.48 Gate.setIR 0,5000,0,6 @08:49:06.59 PuckHeater.configure :default #ambient 21.2C @08:49:06.70 Gate.power :heat,:ON,limit to 5000mA @08:49:07.40 Mounted lysis Cartridge board#Q05092 @08:49:07.48 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:49:07.53 Vacuuming Vent Top cartridge 4 for 30 seconds @08:49:07.57 Air.in :default @08:49:07.69 Air.seek :evac @08:49:22.26 Toroid.in :hold @08:49:22.39 Toroid.seek :cartridge @08:49:24.26 Toroid.in :default @08:49:24.40 Toroid.seek :clear @08:49:26.25 Polling Can every 2:00 @08:49:26.81 PuckHeater.configure :default #ambient 21.4C @08:49:26.92 PuckHeater.seek 41.1C,1:00 @08:49:49.58 PuckHeater.hold 3 seconds,41.1C @08:49:52.83 delayUntil "08:49:55.41" @08:49:55.51 Toroid.in :hold @08:49:55.62 Toroid.seek :bypass @08:49:57.29 Toroid.in :default @08:49:57.40 Toroid.seek :clear @08:49:59.05 delay 2 seconds @08:50:01.08 Air.seek :full @08:50:15.72 Gate.power :heat,:OFF @08:50:15.75 Gate.power :cartridge,:OFF @08:50:15.90 Carousel.in :default @08:50:16.05 Carousel.home.seek 4 @08:50:16.56 Air.in :unlocking @08:50:16.73 Air.seek :clear @08:50:18.47 Lysis1.in :unlocking @08:50:18.67 Lysis1.seek :clear @08:50:18.78 Plunge.in :unlocking @08:50:18.91 Plunge.seek :clear @08:50:20.09 Carousel.seek 5 @08:50:20.70 Lysis1.in :fast @08:50:20.81 Lysis1.seek :locked @08:50:23.46 Plunge.in :default @08:50:23.61 Plunge.seek :locked @08:50:23.79 Air.in :fast @08:50:23.90 Air.seek :locked @08:50:24.91 Carousel.reconfigure :locked @08:50:25.11 Powering cartridge bus @08:50:27.78 Gate.setIR 1750,5000,0,6 @08:50:27.91 Gate.setIR 0,5000,0,6 @08:50:28.03 Gate.power :cartridge,:OFF @08:50:28.62 Mounted archive Cartridge @08:50:28.71 Vacuuming Solid Top cartridge 5 for 30 seconds @08:50:28.85 Toroid.in :hold @08:50:28.96 Toroid.seek :cartridge @08:50:30.84 Toroid.in :default @08:50:30.94 Toroid.seek :clear @08:50:32.80 delay 30 seconds @08:51:02.91 Toroid.in :hold @08:51:03.03 Toroid.seek :bypass @08:51:04.70 Toroid.in :default @08:51:04.81 Toroid.seek :clear @08:51:06.70 Carousel.in :default @08:51:06.87 Carousel.home.seek 5 @08:51:07.34 Air.in :unlocking @08:51:07.50 Air.seek :clear @08:51:08.55 Lysis1.in :unlocking @08:51:08.76 Lysis1.seek :clear @08:51:08.98 Plunge.in :unlocking @08:51:09.10 Plunge.seek :clear @08:51:10.25 Carousel.seek 6 @08:51:10.83 Lysis1.in :fast @08:51:10.93 Lysis1.seek :locked @08:51:13.59 Plunge.in :default @08:51:14.46 Plunge.seek :locked @08:51:14.60 Air.in :fast @08:51:14.69 Air.seek :locked @08:51:15.69 Carousel.reconfigure :locked @08:51:15.90 Powering cartridge bus @08:51:18.57 Gate.setIR 1750,5000,0,6 @08:51:18.70 Gate.setIR 0,5000,0,6 @08:51:18.82 PuckHeater.configure :default #ambient 21.4C @08:51:18.93 Gate.power :heat,:ON,limit to 5000mA @08:51:19.64 Mounted lysis Cartridge board#Q05031 @08:51:19.73 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:51:19.77 Vacuuming Vent Top cartridge 6 for 30 seconds @08:51:19.83 Air.in :default @08:51:19.95 Air.seek :evac @08:51:34.52 Toroid.in :hold @08:51:34.63 Toroid.seek :cartridge @08:51:36.51 Toroid.in :default @08:51:36.62 Toroid.seek :clear @08:51:38.47 Polling Can every 2:00 @08:51:39.03 PuckHeater.configure :default #ambient 21.5C @08:51:39.13 PuckHeater.seek 41.1C,1:00 @08:52:01.85 PuckHeater.hold 3 seconds,41.1C @08:52:05.08 delayUntil "08:52:07.65" @08:52:07.75 Toroid.in :hold @08:52:07.86 Toroid.seek :bypass @08:52:09.54 Toroid.in :default @08:52:09.66 Toroid.seek :clear @08:52:11.31 delay 2 seconds @08:52:13.34 Air.seek :full @08:52:27.97 Gate.power :heat,:OFF @08:52:28.00 Gate.power :cartridge,:OFF @08:52:28.14 Carousel.in :default @08:52:28.30 Carousel.home.seek 6 @08:52:28.76 Air.in :unlocking @08:52:28.93 Air.seek :clear @08:52:30.65 Lysis1.in :unlocking @08:52:30.86 Lysis1.seek :clear @08:52:30.92 Plunge.in :unlocking @08:52:31.10 Plunge.seek :clear @08:52:32.34 Carousel.seek 7 @08:52:32.98 Lysis1.in :fast @08:52:33.08 Lysis1.seek :locked @08:52:35.75 Plunge.in :default @08:52:35.84 Plunge.seek :locked @08:52:36.04 Air.in :fast @08:52:36.14 Air.seek :locked @08:52:37.16 Carousel.reconfigure :locked @08:52:37.36 Powering cartridge bus @08:52:40.03 Gate.setIR 1750,5000,0,6 @08:52:40.17 Gate.setIR 0,5000,0,6 @08:52:40.30 Gate.power :cartridge,:OFF @08:52:40.92 Mounted archive Cartridge @08:52:41.01 Vacuuming Solid Top cartridge 7 for 30 seconds @08:52:41.14 Toroid.in :hold @08:52:41.25 Toroid.seek :cartridge @08:52:43.12 Toroid.in :default @08:52:43.24 Toroid.seek :clear @08:52:45.09 delay 30 seconds @08:53:15.23 Toroid.in :hold @08:53:15.39 Toroid.seek :bypass @08:53:17.06 Toroid.in :default @08:53:17.17 Toroid.seek :clear @08:53:19.04 Carousel.in :default @08:53:19.21 Carousel.home.seek 7 @08:53:19.68 Air.in :unlocking @08:53:19.84 Air.seek :clear @08:53:20.95 Lysis1.in :unlocking @08:53:21.08 Lysis1.seek :clear @08:53:21.29 Plunge.in :unlocking @08:53:21.40 Plunge.seek :clear @08:53:22.54 Carousel.seek 8 @08:53:23.13 Lysis1.in :fast @08:53:23.23 Lysis1.seek :locked @08:53:26.48 Plunge.in :default @08:53:26.73 Plunge.seek :locked @08:53:26.88 Air.in :fast @08:53:26.98 Air.seek :locked @08:53:27.98 Carousel.reconfigure :locked @08:53:28.19 Powering cartridge bus @08:53:30.86 Gate.setIR 1750,5000,0,6 @08:53:31.00 Gate.setIR 0,5000,0,6 @08:53:31.11 PuckHeater.configure :default #ambient 21.5C @08:53:31.22 Gate.power :heat,:ON,limit to 5000mA @08:53:31.93 Mounted lysis Cartridge board#Q05061 @08:53:32.02 Using this cartridge's heat loss curve for HeatingAccelInCarousel @08:53:32.08 Vacuuming Vent Top cartridge 8 for 30 seconds @08:53:32.11 Air.in :default @08:53:32.22 Air.seek :evac @08:53:46.81 Toroid.in :hold @08:53:46.92 Toroid.seek :cartridge @08:53:48.80 Toroid.in :default @08:53:48.90 Toroid.seek :clear @08:53:50.75 Polling Can every 2:00 @08:53:51.30 PuckHeater.configure :default #ambient 21.6C @08:53:51.41 PuckHeater.seek 41.3C,1:00 @08:54:14.07 PuckHeater.hold 3 seconds,41.3C @08:54:17.24 delayUntil "08:54:19.94" @08:54:20.04 Toroid.in :hold @08:54:20.15 Toroid.seek :bypass @08:54:21.83 Toroid.in :default @08:54:21.93 Toroid.seek :clear @08:54:23.58 delay 2 seconds @08:54:25.61 Air.seek :full @08:54:40.25 Gate.power :heat,:OFF @08:54:40.27 Gate.power :cartridge,:OFF @08:54:40.45 Carousel.in :default @08:54:40.61 Carousel.home.seek 8 @08:54:41.73 Air.in :unlocking @08:54:41.90 Air.seek :clear @08:54:42.94 Lysis1.in :unlocking @08:54:43.09 Lysis1.seek :clear @08:54:43.24 Plunge.in :unlocking @08:54:43.34 Plunge.seek :clear @08:54:44.50 Carousel.seek 9 @08:54:45.07 Lysis1.in :fast @08:54:45.17 Lysis1.seek :locked @08:54:47.86 Plunge.in :default @08:54:48.01 Plunge.seek :locked @08:54:48.15 Air.in :fast @08:54:48.25 Air.seek :locked @08:54:49.27 Carousel.reconfigure :locked @08:54:49.47 Powering cartridge bus @08:54:52.14 Gate.setIR 1750,5000,0,6 @08:54:52.28 Gate.setIR 0,5000,0,6 @08:54:52.42 Gate.power :cartridge,:OFF @08:54:53.01 Mounted archive Cartridge @08:54:53.09 Vacuuming Solid Top cartridge 9 for 30 seconds @08:54:53.24 Toroid.in :hold @08:54:53.34 Toroid.seek :cartridge @08:54:55.21 Toroid.in :default @08:54:55.32 Toroid.seek :clear @08:54:57.14 delay 30 seconds @08:55:27.26 Toroid.in :hold @08:55:27.37 Toroid.seek :bypass @08:55:29.06 Toroid.in :default @08:55:29.16 Toroid.seek :clear @08:55:31.02 Carousel.in :default @08:55:31.20 Carousel.home.seek 9 @08:55:31.73 Air.in :unlocking @08:55:31.94 Air.seek :clear @08:55:33.01 Lysis1.in :unlocking @08:55:33.18 Lysis1.seek :clear @08:55:33.34 Plunge.in :unlocking @08:55:33.42 Plunge.seek :clear @08:55:34.60 Carousel.seek 10 @08:55:35.18 Lysis1.in :fast @08:55:35.27 Lysis1.seek :locked @08:55:38.61 Plunge.in :default @08:55:38.73 Plunge.seek :locked @08:55:38.93 Air.in :fast @08:55:39.01 Air.seek :locked @08:55:40.03 Carousel.reconfigure :locked @08:55:40.23 Powering cartridge bus @08:55:42.90 Gate.setIR 1750,5000,0,6 @08:55:43.04 Gate.setIR 0,5000,0,6 @08:55:43.15 PuckHeater.configure :default #ambient 21.6C @08:55:43.27 Gate.power :heat,:ON,limit to 5000mA @08:55:43.98 Mounted lysis Cartridge board#Q05080 @08:55:44.06 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:55:44.10 Vacuuming Vent Top cartridge 10 for 30 seconds @08:55:44.17 Air.in :default @08:55:44.28 Air.seek :evac @08:55:58.89 Toroid.in :hold @08:55:58.99 Toroid.seek :cartridge @08:56:00.87 Toroid.in :default @08:56:00.97 Toroid.seek :clear @08:56:02.79 Polling Can every 2:00 @08:56:03.35 PuckHeater.configure :default #ambient 21.7C @08:56:03.44 PuckHeater.seek 41.1C,1:00 @08:56:26.12 PuckHeater.hold 3 seconds,41.1C @08:56:29.27 delayUntil "08:56:32.01" @08:56:32.11 Toroid.in :hold @08:56:32.21 Toroid.seek :bypass @08:56:33.88 Toroid.in :default @08:56:33.96 Toroid.seek :clear @08:56:35.61 delay 2 seconds @08:56:37.64 Air.seek :full @08:56:52.29 Gate.power :heat,:OFF @08:56:52.32 Gate.power :cartridge,:OFF @08:56:52.49 Carousel.in :default @08:56:52.64 Carousel.home.seek 10 @08:56:53.75 Air.in :unlocking @08:56:53.90 Air.seek :clear @08:56:54.96 Lysis1.in :unlocking @08:56:55.16 Lysis1.seek :clear @08:56:55.26 Plunge.in :unlocking @08:56:55.38 Plunge.seek :clear @08:56:56.57 Carousel.seek 11 @08:56:57.16 Lysis1.in :fast @08:56:57.25 Lysis1.seek :locked @08:56:59.93 Plunge.in :default @08:57:00.07 Plunge.seek :locked @08:57:00.22 Air.in :fast @08:57:00.32 Air.seek :locked @08:57:01.35 Carousel.reconfigure :locked @08:57:01.54 Powering cartridge bus @08:57:04.22 Gate.setIR 1750,5000,0,6 @08:57:04.37 Gate.setIR 0,5000,0,6 @08:57:04.49 Gate.power :cartridge,:OFF @08:57:05.08 Mounted archive Cartridge @08:57:05.16 Vacuuming Solid Top cartridge 11 for 30 seconds @08:57:05.30 Toroid.in :hold @08:57:05.40 Toroid.seek :cartridge @08:57:07.28 Toroid.in :default @08:57:07.37 Toroid.seek :clear @08:57:09.19 delay 30 seconds @08:57:39.36 Toroid.in :hold @08:57:39.46 Toroid.seek :bypass @08:57:41.13 Toroid.in :default @08:57:41.23 Toroid.seek :clear @08:57:43.08 Carousel.in :default @08:57:43.24 Carousel.home.seek 11 @08:57:43.69 Air.in :unlocking @08:57:43.87 Air.seek :clear @08:57:44.92 Lysis1.in :unlocking @08:57:45.05 Lysis1.seek :clear @08:57:45.22 Plunge.in :unlocking @08:57:45.32 Plunge.seek :clear @08:57:46.49 Carousel.seek 12 @08:57:47.60 Lysis1.in :fast @08:57:47.80 Lysis1.seek :locked @08:57:50.49 Plunge.in :default @08:57:50.60 Plunge.seek :locked @08:57:50.81 Air.in :fast @08:57:50.90 Air.seek :locked @08:57:51.91 Carousel.reconfigure :locked @08:57:52.10 Powering cartridge bus @08:57:54.77 Gate.setIR 1750,5000,0,6 @08:57:54.90 Gate.setIR 0,5000,0,6 @08:57:55.01 PuckHeater.configure :default #ambient 21.7C @08:57:55.12 Gate.power :heat,:ON,limit to 5000mA @08:57:55.83 Mounted lysis Cartridge board#Q05038 @08:57:55.90 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:57:55.95 Vacuuming Vent Top cartridge 12 for 30 seconds @08:57:56.00 Air.in :default @08:57:56.10 Air.seek :evac @08:58:10.69 Toroid.in :hold @08:58:10.79 Toroid.seek :cartridge @08:58:12.66 Toroid.in :default @08:58:12.75 Toroid.seek :clear @08:58:14.60 Polling Can every 2:00 @08:58:15.16 PuckHeater.configure :default #ambient 21.7C @08:58:15.26 PuckHeater.seek 41.1C,1:00 @08:58:37.93 PuckHeater.hold 3 seconds,41.1C @08:58:41.07 delayUntil "08:58:43.81" @08:58:43.91 Toroid.in :hold @08:58:44.01 Toroid.seek :bypass @08:58:45.68 Toroid.in :default @08:58:45.78 Toroid.seek :clear @08:58:47.43 delay 2 seconds @08:58:49.46 Air.seek :full @08:59:04.10 Gate.power :heat,:OFF @08:59:04.13 Gate.power :cartridge,:OFF @08:59:04.26 Carousel.in :default @08:59:04.45 Carousel.home.seek 12 @08:59:05.45 Air.in :unlocking @08:59:05.61 Air.seek :clear @08:59:06.65 Lysis1.in :unlocking @08:59:06.81 Lysis1.seek :clear @08:59:06.96 Plunge.in :unlocking @08:59:07.06 Plunge.seek :clear @08:59:08.22 Carousel.seek 13 @08:59:08.82 Lysis1.in :fast @08:59:08.92 Lysis1.seek :locked @08:59:11.60 Plunge.in :default @08:59:11.70 Plunge.seek :locked @08:59:11.89 Air.in :fast @08:59:11.98 Air.seek :locked @08:59:12.97 Carousel.reconfigure :locked @08:59:13.15 Powering cartridge bus @08:59:15.82 Gate.setIR 1750,5000,0,6 @08:59:15.95 Gate.setIR 0,5000,0,6 @08:59:16.07 Gate.power :cartridge,:OFF @08:59:16.66 Mounted archive Cartridge @08:59:16.74 Vacuuming Solid Top cartridge 13 for 30 seconds @08:59:16.87 Toroid.in :hold @08:59:16.96 Toroid.seek :cartridge @08:59:18.83 Toroid.in :default @08:59:18.94 Toroid.seek :clear @08:59:20.78 delay 30 seconds @08:59:50.90 Toroid.in :hold @08:59:50.99 Toroid.seek :bypass @08:59:52.67 Toroid.in :default @08:59:52.77 Toroid.seek :clear @08:59:54.63 Carousel.in :default @08:59:54.79 Carousel.home.seek 13 @08:59:55.25 Air.in :unlocking @08:59:55.39 Air.seek :clear @08:59:56.49 Lysis1.in :unlocking @08:59:56.66 Lysis1.seek :clear @08:59:56.73 Plunge.in :unlocking @08:59:56.90 Plunge.seek :clear @08:59:58.13 Carousel.seek 14 @08:59:59.24 Lysis1.in :fast @08:59:59.43 Lysis1.seek :locked @09:00:02.10 Plunge.in :default @09:00:02.21 Plunge.seek :locked @09:00:02.45 Air.in :fast @09:00:02.54 Air.seek :locked @09:00:03.55 Carousel.reconfigure :locked @09:00:03.76 Powering cartridge bus @09:00:06.43 Gate.setIR 1750,5000,0,6 @09:00:06.57 Gate.setIR 0,5000,0,6 @09:00:06.67 PuckHeater.configure :default #ambient 21.7C @09:00:06.78 Gate.power :heat,:ON,limit to 5000mA @09:00:07.49 Mounted lysis Cartridge board#Q05037 @09:00:07.56 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:00:07.61 Vacuuming Vent Top cartridge 14 for 30 seconds @09:00:07.67 Air.in :default @09:00:07.77 Air.seek :evac @09:00:22.39 Toroid.in :hold @09:00:22.49 Toroid.seek :cartridge @09:00:24.40 Toroid.in :default @09:00:24.50 Toroid.seek :clear @09:00:26.38 Polling Can every 2:00 @09:00:26.94 PuckHeater.configure :default #ambient 21.7C @09:00:27.03 PuckHeater.seek 41.1C,1:00 @09:00:49.70 PuckHeater.hold 3 seconds,41.1C @09:00:52.93 delayUntil "09:00:55.51" @09:00:55.63 Toroid.in :hold @09:00:55.73 Toroid.seek :bypass @09:00:57.40 Toroid.in :default @09:00:57.51 Toroid.seek :clear @09:00:59.17 delay 2 seconds @09:01:01.20 Air.seek :full @09:01:15.85 Gate.power :heat,:OFF @09:01:15.87 Gate.power :cartridge,:OFF @09:01:16.01 Carousel.in :default @09:01:16.76 Carousel.home.seek 14 @09:01:17.33 Air.in :unlocking @09:01:17.52 Air.seek :clear @09:01:18.56 Lysis1.in :unlocking @09:01:18.75 Lysis1.seek :clear @09:01:18.84 Plunge.in :unlocking @09:01:19.02 Plunge.seek :clear @09:01:20.24 Carousel.seek 15 @09:01:20.84 Lysis1.in :fast @09:01:20.95 Lysis1.seek :locked @09:01:23.63 Plunge.in :default @09:01:23.73 Plunge.seek :locked @09:01:23.94 Air.in :fast @09:01:24.04 Air.seek :locked @09:01:25.05 Carousel.reconfigure :locked @09:01:25.24 Powering cartridge bus @09:01:27.95 Gate.setIR 1750,5000,0,6 @09:01:28.08 Gate.setIR 0,5000,0,6 @09:01:28.20 Gate.power :cartridge,:OFF @09:01:28.80 Mounted archive Cartridge @09:01:28.89 Vacuuming Solid Top cartridge 15 for 30 seconds @09:01:29.03 Toroid.in :hold @09:01:29.13 Toroid.seek :cartridge @09:01:31.01 Toroid.in :default @09:01:31.11 Toroid.seek :clear @09:01:32.94 delay 30 seconds @09:02:03.06 Toroid.in :hold @09:02:03.17 Toroid.seek :bypass @09:02:04.85 Toroid.in :default @09:02:04.97 Toroid.seek :clear @09:02:06.83 Carousel.in :default @09:02:06.99 Carousel.home.seek 15 @09:02:07.46 Air.in :unlocking @09:02:07.61 Air.seek :clear @09:02:08.66 Lysis1.in :unlocking @09:02:08.83 Lysis1.seek :clear @09:02:08.97 Plunge.in :unlocking @09:02:09.07 Plunge.seek :clear @09:02:10.83 Carousel.seek 16 @09:02:11.51 Lysis1.in :fast @09:02:11.62 Lysis1.seek :locked @09:02:14.28 Plunge.in :default @09:02:14.40 Plunge.seek :locked @09:02:14.59 Air.in :fast @09:02:14.69 Air.seek :locked @09:02:15.70 Carousel.reconfigure :locked @09:02:15.91 Powering cartridge bus @09:02:18.58 Gate.setIR 1750,5000,0,6 @09:02:18.72 Gate.setIR 0,5000,0,6 @09:02:18.83 PuckHeater.configure :default #ambient 21.7C @09:02:18.94 Gate.power :heat,:ON,limit to 5000mA @09:02:19.65 Mounted lysis Cartridge board#Q05054 @09:02:19.72 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:02:19.79 Vacuuming Vent Top cartridge 16 for 30 seconds @09:02:19.82 Air.in :default @09:02:19.94 Air.seek :evac @09:02:34.53 Toroid.in :hold @09:02:34.64 Toroid.seek :cartridge @09:02:36.51 Toroid.in :default @09:02:36.62 Toroid.seek :clear @09:02:38.46 Polling Can every 2:00 @09:02:39.02 PuckHeater.configure :default #ambient 21.8C @09:02:39.12 PuckHeater.seek 41.1C,1:00 @09:03:01.81 PuckHeater.hold 3 seconds,41.1C @09:03:05.08 delayUntil "09:03:07.66" @09:03:07.76 Toroid.in :hold @09:03:07.87 Toroid.seek :bypass @09:03:09.55 Toroid.in :default @09:03:09.66 Toroid.seek :clear @09:03:11.31 delay 2 seconds @09:03:13.34 Air.seek :full @09:03:27.99 Gate.power :heat,:OFF @09:03:28.02 Gate.power :cartridge,:OFF @09:03:28.74 Carousel.in :default @09:03:29.00 Carousel.home.seek 16 @09:03:29.48 Air.in :unlocking @09:03:29.67 Air.seek :clear @09:03:30.72 Lysis1.in :unlocking @09:03:30.94 Lysis1.seek :clear @09:03:31.01 Plunge.in :unlocking @09:03:31.17 Plunge.seek :clear @09:03:32.42 Carousel.seek 17 @09:03:33.03 Lysis1.in :fast @09:03:33.13 Lysis1.seek :locked @09:03:35.79 Plunge.in :default @09:03:35.91 Plunge.seek :locked @09:03:36.08 Air.in :fast @09:03:36.18 Air.seek :locked @09:03:37.19 Carousel.reconfigure :locked @09:03:37.39 Powering cartridge bus @09:03:40.06 Gate.setIR 1750,5000,0,6 @09:03:40.19 Gate.setIR 0,5000,0,6 @09:03:40.31 Gate.power :cartridge,:OFF @09:03:40.94 Mounted archive Cartridge @09:03:41.03 Vacuuming Solid Top cartridge 17 for 30 seconds @09:03:41.17 Toroid.in :hold @09:03:41.29 Toroid.seek :cartridge @09:03:43.16 Toroid.in :default @09:03:43.27 Toroid.seek :clear @09:03:45.12 delay 30 seconds @09:04:15.23 Toroid.in :hold @09:04:15.33 Toroid.seek :bypass @09:04:17.01 Toroid.in :default @09:04:17.10 Toroid.seek :clear @09:04:18.98 Carousel.in :default @09:04:19.15 Carousel.home.seek 17 @09:04:19.63 Air.in :unlocking @09:04:19.78 Air.seek :clear @09:04:20.82 Lysis1.in :unlocking @09:04:21.00 Lysis1.seek :clear @09:04:21.08 Plunge.in :unlocking @09:04:21.22 Plunge.seek :clear @09:04:22.94 Carousel.seek 18 @09:04:23.53 Lysis1.in :fast @09:04:23.64 Lysis1.seek :locked @09:04:26.30 Plunge.in :default @09:04:26.44 Plunge.seek :locked @09:04:26.67 Air.in :fast @09:04:26.77 Air.seek :locked @09:04:27.79 Carousel.reconfigure :locked @09:04:28.00 Powering cartridge bus @09:04:30.67 Gate.setIR 1750,5000,0,6 @09:04:30.80 Gate.setIR 0,5000,0,6 @09:04:30.91 PuckHeater.configure :default #ambient 21.8C @09:04:31.02 Gate.power :heat,:ON,limit to 5000mA @09:04:31.74 Mounted lysis Cartridge board#Q05063 @09:04:31.82 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:04:31.87 Vacuuming Vent Top cartridge 18 for 30 seconds @09:04:31.95 Air.in :default @09:04:32.06 Air.seek :evac @09:04:46.65 Toroid.in :hold @09:04:46.76 Toroid.seek :cartridge @09:04:48.64 Toroid.in :default @09:04:48.75 Toroid.seek :clear @09:04:50.60 Polling Can every 2:00 @09:04:51.16 PuckHeater.configure :default #ambient 21.9C @09:04:51.26 PuckHeater.seek 41.1C,1:00 @09:05:13.91 PuckHeater.hold 3 seconds,41.1C @09:05:17.13 delayUntil "09:05:19.78" @09:05:19.88 Toroid.in :hold @09:05:19.98 Toroid.seek :bypass @09:05:21.66 Toroid.in :default @09:05:21.76 Toroid.seek :clear @09:05:23.41 delay 2 seconds @09:05:25.44 Air.seek :full @09:05:40.66 Gate.power :heat,:OFF @09:05:40.79 Gate.power :cartridge,:OFF @09:05:40.94 Carousel.in :default @09:05:41.09 Carousel.home.seek 18 @09:05:41.55 Air.in :unlocking @09:05:41.73 Air.seek :clear @09:05:42.80 Lysis1.in :unlocking @09:05:42.98 Lysis1.seek :clear @09:05:43.21 Plunge.in :unlocking @09:05:43.34 Plunge.seek :clear @09:05:44.51 Carousel.seek 19 @09:05:45.10 Lysis1.in :fast @09:05:45.21 Lysis1.seek :locked @09:05:47.89 Plunge.in :default @09:05:48.05 Plunge.seek :locked @09:05:48.21 Air.in :fast @09:05:48.31 Air.seek :locked @09:05:49.36 Carousel.reconfigure :locked @09:05:49.56 Powering cartridge bus @09:05:52.23 Gate.setIR 1750,5000,0,6 @09:05:52.38 Gate.setIR 0,5000,0,6 @09:05:52.50 Gate.power :cartridge,:OFF @09:05:53.09 Mounted archive Cartridge @09:05:53.19 Vacuuming Solid Top cartridge 19 for 30 seconds @09:05:53.32 Toroid.in :hold @09:05:53.41 Toroid.seek :cartridge @09:05:55.29 Toroid.in :default @09:05:55.40 Toroid.seek :clear @09:05:57.25 delay 30 seconds @09:06:27.37 Toroid.in :hold @09:06:27.48 Toroid.seek :bypass @09:06:29.15 Toroid.in :default @09:06:29.26 Toroid.seek :clear @09:06:31.12 Carousel.in :default @09:06:31.29 Carousel.home.seek 19 @09:06:31.75 Air.in :unlocking @09:06:31.88 Air.seek :clear @09:06:32.96 Lysis1.in :unlocking @09:06:33.16 Lysis1.seek :clear @09:06:33.83 Plunge.in :unlocking @09:06:34.04 Plunge.seek :clear @09:06:35.19 Carousel.seek 20 @09:06:35.79 Lysis1.in :fast @09:06:35.90 Lysis1.seek :locked @09:06:38.57 Plunge.in :default @09:06:38.70 Plunge.seek :locked @09:06:38.88 Air.in :fast @09:06:38.98 Air.seek :locked @09:06:39.99 Carousel.reconfigure :locked @09:06:40.21 Powering cartridge bus @09:06:42.88 Gate.setIR 1750,5000,0,6 @09:06:43.02 Gate.setIR 0,5000,0,6 @09:06:43.13 PuckHeater.configure :default #ambient 21.9C @09:06:43.25 Gate.power :heat,:ON,limit to 5000mA @09:06:43.96 Mounted lysis Cartridge board#Q05042 @09:06:44.03 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:06:44.11 Vacuuming Vent Top cartridge 20 for 30 seconds @09:06:44.14 Air.in :default @09:06:44.27 Air.seek :evac @09:06:58.87 Toroid.in :hold @09:06:58.98 Toroid.seek :cartridge @09:07:00.86 Toroid.in :default @09:07:00.96 Toroid.seek :clear @09:07:02.80 Polling Can every 2:00 @09:07:03.36 PuckHeater.configure :default #ambient 22.0C @09:07:03.46 PuckHeater.seek 41.1C,1:00 @09:07:26.13 PuckHeater.hold 3 seconds,41.1C @09:07:29.31 delayUntil "09:07:32.00" @09:07:32.10 Toroid.in :hold @09:07:32.21 Toroid.seek :bypass @09:07:33.90 Toroid.in :default @09:07:34.00 Toroid.seek :clear @09:07:35.64 delay 2 seconds @09:07:37.67 Air.seek :full @09:07:52.32 Gate.power :heat,:OFF @09:07:52.46 Gate.power :cartridge,:OFF @09:07:52.62 Carousel.in :default @09:07:52.78 Carousel.home.seek 20 @09:07:53.24 Air.in :unlocking @09:07:53.40 Air.seek :clear @09:07:54.50 Lysis1.in :unlocking @09:07:54.69 Lysis1.seek :clear @09:07:54.76 Plunge.in :unlocking @09:07:54.94 Plunge.seek :clear @09:07:56.18 Carousel.seek 21 @09:07:56.77 Lysis1.in :fast @09:07:56.86 Lysis1.seek :locked @09:07:59.54 Plunge.in :default @09:07:59.63 Plunge.seek :locked @09:07:59.83 Air.in :fast @09:07:59.94 Air.seek :locked @09:08:00.95 Carousel.reconfigure :locked @09:08:01.15 Powering cartridge bus @09:08:03.81 Gate.setIR 1750,5000,0,6 @09:08:03.94 Gate.setIR 0,5000,0,6 @09:08:04.06 Gate.power :cartridge,:OFF @09:08:04.65 Mounted archive Cartridge @09:08:04.76 Vacuuming Solid Top cartridge 21 for 30 seconds @09:08:04.90 Toroid.in :hold @09:08:05.00 Toroid.seek :cartridge @09:08:06.88 Toroid.in :default @09:08:06.98 Toroid.seek :clear @09:08:08.83 delay 30 seconds @09:08:38.94 Toroid.in :hold @09:08:39.05 Toroid.seek :bypass @09:08:40.74 Toroid.in :default @09:08:40.85 Toroid.seek :clear @09:08:42.74 Carousel.in :default @09:08:42.91 Carousel.home.seek 21 @09:08:43.37 Air.in :unlocking @09:08:43.58 Air.seek :clear @09:08:44.63 Lysis1.in :unlocking @09:08:45.37 Lysis1.seek :clear @09:08:45.61 Plunge.in :unlocking @09:08:45.70 Plunge.seek :clear @09:08:46.89 Carousel.seek 22 @09:08:47.49 Lysis1.in :fast @09:08:47.59 Lysis1.seek :locked @09:08:50.27 Plunge.in :default @09:08:50.40 Plunge.seek :locked @09:08:50.59 Air.in :fast @09:08:50.69 Air.seek :locked @09:08:51.67 Carousel.reconfigure :locked @09:08:51.88 Powering cartridge bus @09:08:54.55 Gate.setIR 1750,5000,0,6 @09:08:54.68 Gate.setIR 0,5000,0,6 @09:08:54.78 PuckHeater.configure :default #ambient 22.0C @09:08:54.88 Gate.power :heat,:ON,limit to 5000mA @09:08:55.59 Mounted lysis Cartridge board#Q05090 @09:08:55.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:08:55.73 Vacuuming Vent Top cartridge 22 for 30 seconds @09:08:55.76 Air.in :default @09:08:55.88 Air.seek :evac @09:09:10.49 Toroid.in :hold @09:09:10.60 Toroid.seek :cartridge @09:09:12.47 Toroid.in :default @09:09:12.58 Toroid.seek :clear @09:09:14.43 Polling Can every 2:00 @09:09:14.99 PuckHeater.configure :default #ambient 22.1C @09:09:15.08 PuckHeater.seek 41.1C,1:00 @09:09:37.81 PuckHeater.hold 3 seconds,41.1C @09:09:41.02 delayUntil "09:09:43.62" @09:09:43.72 Toroid.in :hold @09:09:43.83 Toroid.seek :bypass @09:09:45.52 Toroid.in :default @09:09:45.62 Toroid.seek :clear @09:09:47.28 delay 2 seconds @09:09:49.90 Air.seek :full @09:10:04.66 Gate.power :heat,:OFF @09:10:04.68 Gate.power :cartridge,:OFF @09:10:04.84 Carousel.in :default @09:10:04.99 Carousel.home.seek 22 @09:10:05.48 Air.in :unlocking @09:10:05.64 Air.seek :clear @09:10:06.70 Lysis1.in :unlocking @09:10:06.89 Lysis1.seek :clear @09:10:06.96 Plunge.in :unlocking @09:10:07.15 Plunge.seek :clear @09:10:08.41 Carousel.seek 23 @09:10:09.00 Lysis1.in :fast @09:10:09.11 Lysis1.seek :locked @09:10:11.77 Plunge.in :default @09:10:11.93 Plunge.seek :locked @09:10:12.08 Air.in :fast @09:10:12.18 Air.seek :locked @09:10:13.20 Carousel.reconfigure :locked @09:10:13.41 Powering cartridge bus @09:10:16.07 Gate.setIR 1750,5000,0,6 @09:10:16.20 Gate.setIR 0,5000,0,6 @09:10:16.32 Gate.power :cartridge,:OFF @09:10:16.95 Mounted archive Cartridge @09:10:17.05 Vacuuming Solid Top cartridge 23 for 30 seconds @09:10:17.18 Toroid.in :hold @09:10:17.27 Toroid.seek :cartridge @09:10:19.13 Toroid.in :default @09:10:19.23 Toroid.seek :clear @09:10:21.07 delay 30 seconds @09:10:51.19 Toroid.in :hold @09:10:51.30 Toroid.seek :bypass @09:10:52.98 Toroid.in :default @09:10:53.08 Toroid.seek :clear @09:10:54.95 Carousel.in :default @09:10:55.12 Carousel.home.seek 23 @09:10:55.59 Air.in :unlocking @09:10:55.77 Air.seek :clear @09:10:57.48 Lysis1.in :unlocking @09:10:57.68 Lysis1.seek :clear @09:10:57.79 Plunge.in :unlocking @09:10:57.93 Plunge.seek :clear @09:10:59.13 Carousel.seek 24 @09:10:59.71 Lysis1.in :fast @09:10:59.80 Lysis1.seek :locked @09:11:02.49 Plunge.in :default @09:11:02.62 Plunge.seek :locked @09:11:02.79 Air.in :fast @09:11:02.90 Air.seek :locked @09:11:03.91 Carousel.reconfigure :locked @09:11:04.11 Powering cartridge bus @09:11:06.78 Gate.setIR 1750,5000,0,6 @09:11:06.91 Gate.setIR 0,5000,0,6 @09:11:07.02 PuckHeater.configure :default #ambient 22.1C @09:11:07.14 Gate.power :heat,:ON,limit to 5000mA @09:11:07.85 Mounted lysis Cartridge board#Q05076 @09:11:07.92 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:11:07.98 Vacuuming Vent Top cartridge 24 for 30 seconds @09:11:08.02 Air.in :default @09:11:08.15 Air.seek :evac @09:11:22.74 Toroid.in :hold @09:11:22.85 Toroid.seek :cartridge @09:11:24.72 Toroid.in :default @09:11:24.83 Toroid.seek :clear @09:11:26.68 Polling Can every 2:00 @09:11:27.23 PuckHeater.configure :default #ambient 22.2C @09:11:27.33 PuckHeater.seek 41.1C,1:00 @09:11:49.96 PuckHeater.hold 3 seconds,41.1C @09:11:53.17 delayUntil "09:11:55.87" @09:11:55.97 Toroid.in :hold @09:11:56.08 Toroid.seek :bypass @09:11:57.75 Toroid.in :default @09:11:58.44 Toroid.seek :clear @09:12:00.20 delay 2 seconds @09:12:02.23 Air.seek :full @09:12:16.89 Gate.power :heat,:OFF @09:12:16.91 Gate.power :cartridge,:OFF @09:12:17.07 Carousel.in :default @09:12:17.23 Carousel.home.seek 24 @09:12:17.71 Air.in :unlocking @09:12:17.87 Air.seek :clear @09:12:18.91 Lysis1.in :unlocking @09:12:19.08 Lysis1.seek :clear @09:12:19.24 Plunge.in :unlocking @09:12:19.33 Plunge.seek :clear @09:12:20.50 Carousel.seek 25 @09:12:21.08 Lysis1.in :fast @09:12:21.18 Lysis1.seek :locked @09:12:23.86 Plunge.in :default @09:12:23.95 Plunge.seek :locked @09:12:24.15 Air.in :fast @09:12:24.26 Air.seek :locked @09:12:25.27 Carousel.reconfigure :locked @09:12:25.46 Powering cartridge bus @09:12:28.13 Gate.setIR 1750,5000,0,6 @09:12:28.26 Gate.setIR 0,5000,0,6 @09:12:28.41 Gate.power :cartridge,:OFF @09:12:29.00 Mounted archive Cartridge @09:12:29.09 Vacuuming Solid Top cartridge 25 for 30 seconds @09:12:29.22 Toroid.in :hold @09:12:29.31 Toroid.seek :cartridge @09:12:31.20 Toroid.in :default @09:12:31.31 Toroid.seek :clear @09:12:33.17 delay 30 seconds @09:13:03.29 Toroid.in :hold @09:13:03.39 Toroid.seek :bypass @09:13:05.07 Toroid.in :default @09:13:05.17 Toroid.seek :clear @09:13:07.04 Carousel.in :default @09:13:07.20 Carousel.home.seek 25 @09:13:07.66 Air.in :unlocking @09:13:07.85 Air.seek :clear @09:13:09.61 Lysis1.in :unlocking @09:13:09.77 Lysis1.seek :clear @09:13:09.92 Plunge.in :unlocking @09:13:10.04 Plunge.seek :clear @09:13:11.21 Carousel.seek 26 @09:13:11.80 Lysis1.in :fast @09:13:11.90 Lysis1.seek :locked @09:13:14.57 Plunge.in :default @09:13:14.74 Plunge.seek :locked @09:13:14.87 Air.in :fast @09:13:14.97 Air.seek :locked @09:13:15.99 Carousel.reconfigure :locked @09:13:16.21 Powering cartridge bus @09:13:18.87 Gate.setIR 1750,5000,0,6 @09:13:19.00 Gate.setIR 0,5000,0,6 @09:13:19.11 PuckHeater.configure :default #ambient 22.2C @09:13:19.23 Gate.power :heat,:ON,limit to 5000mA @09:13:19.94 Mounted lysis Cartridge board#Q05057 @09:13:20.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:13:20.07 Vacuuming Vent Top cartridge 26 for 30 seconds @09:13:20.12 Air.in :default @09:13:20.25 Air.seek :evac @09:13:34.84 Toroid.in :hold @09:13:34.93 Toroid.seek :cartridge @09:13:36.80 Toroid.in :default @09:13:36.91 Toroid.seek :clear @09:13:38.76 Polling Can every 2:00 @09:13:39.32 PuckHeater.configure :default #ambient 22.2C @09:13:39.42 PuckHeater.seek 41.1C,1:00 @09:14:02.09 PuckHeater.hold 3 seconds,41.1C @09:14:05.31 delayUntil "09:14:07.95" @09:14:08.06 Toroid.in :hold @09:14:08.16 Toroid.seek :bypass @09:14:09.84 Toroid.in :default @09:14:10.04 Toroid.seek :clear @09:14:11.66 delay 2 seconds @09:14:13.70 Air.seek :full @09:14:28.35 Gate.power :heat,:OFF @09:14:28.39 Gate.power :cartridge,:OFF @09:14:28.55 Carousel.in :default @09:14:28.71 Carousel.home.seek 26 @09:14:29.19 Air.in :unlocking @09:14:29.35 Air.seek :clear @09:14:30.42 Lysis1.in :unlocking @09:14:30.62 Lysis1.seek :clear @09:14:30.69 Plunge.in :unlocking @09:14:30.88 Plunge.seek :clear @09:14:32.13 Carousel.seek 27 @09:14:32.72 Lysis1.in :fast @09:14:32.82 Lysis1.seek :locked @09:14:35.49 Plunge.in :default @09:14:35.64 Plunge.seek :locked @09:14:35.78 Air.in :fast @09:14:35.87 Air.seek :locked @09:14:36.88 Carousel.reconfigure :locked @09:14:37.09 Powering cartridge bus @09:14:39.76 Gate.setIR 1750,5000,0,6 @09:14:39.90 Gate.setIR 0,5000,0,6 @09:14:40.01 Gate.power :cartridge,:OFF @09:14:40.62 Mounted archive Cartridge @09:14:40.72 Vacuuming Solid Top cartridge 27 for 30 seconds @09:14:40.86 Toroid.in :hold @09:14:40.97 Toroid.seek :cartridge @09:14:42.85 Toroid.in :default @09:14:42.95 Toroid.seek :clear @09:14:44.80 delay 30 seconds @09:15:14.91 Toroid.in :hold @09:15:15.02 Toroid.seek :bypass @09:15:16.71 Toroid.in :default @09:15:16.83 Toroid.seek :clear @09:15:18.70 Carousel.in :default @09:15:18.87 Carousel.home.seek 27 @09:15:19.34 Air.in :unlocking @09:15:20.03 Air.seek :clear @09:15:21.18 Lysis1.in :unlocking @09:15:21.39 Lysis1.seek :clear @09:15:21.50 Plunge.in :unlocking @09:15:21.64 Plunge.seek :clear @09:15:22.83 Carousel.seek 28 @09:15:23.41 Lysis1.in :fast @09:15:23.51 Lysis1.seek :locked @09:15:26.19 Plunge.in :default @09:15:26.30 Plunge.seek :locked @09:15:26.52 Air.in :fast @09:15:26.61 Air.seek :locked @09:15:27.62 Carousel.reconfigure :locked @09:15:27.84 Powering cartridge bus @09:15:30.51 Gate.setIR 1750,5000,0,6 @09:15:30.64 Gate.setIR 0,5000,0,6 @09:15:30.75 PuckHeater.configure :default #ambient 22.2C @09:15:30.87 Gate.power :heat,:ON,limit to 5000mA @09:15:31.58 Mounted lysis Cartridge board#Q05045 @09:15:31.66 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:15:31.70 Vacuuming Vent Top cartridge 28 for 30 seconds @09:15:31.76 Air.in :default @09:15:31.87 Air.seek :evac @09:15:46.48 Toroid.in :hold @09:15:46.58 Toroid.seek :cartridge @09:15:48.46 Toroid.in :default @09:15:48.55 Toroid.seek :clear @09:15:50.41 Polling Can every 2:00 @09:15:50.97 PuckHeater.configure :default #ambient 22.3C @09:15:51.07 PuckHeater.seek 41.1C,1:00 @09:16:13.84 PuckHeater.hold 3 seconds,41.1C @09:16:16.99 delayUntil "09:16:19.60" @09:16:19.70 Toroid.in :hold @09:16:20.35 Toroid.seek :bypass @09:16:22.15 Toroid.in :default @09:16:22.25 Toroid.seek :clear @09:16:23.91 delay 2 seconds @09:16:25.94 Air.seek :full @09:16:40.59 Gate.power :heat,:OFF @09:16:40.62 Gate.power :cartridge,:OFF @09:16:40.78 Carousel.in :default @09:16:40.93 Carousel.home.seek 28 @09:16:41.39 Air.in :unlocking @09:16:41.54 Air.seek :clear @09:16:42.59 Lysis1.in :unlocking @09:16:42.76 Lysis1.seek :clear @09:16:42.92 Plunge.in :unlocking @09:16:43.03 Plunge.seek :clear @09:16:44.17 Carousel.seek 29 @09:16:44.76 Lysis1.in :fast @09:16:44.86 Lysis1.seek :locked @09:16:47.53 Plunge.in :default @09:16:47.67 Plunge.seek :locked @09:16:47.83 Air.in :fast @09:16:47.94 Air.seek :locked @09:16:48.94 Carousel.reconfigure :locked @09:16:49.14 Powering cartridge bus @09:16:51.82 Gate.setIR 1750,5000,0,6 @09:16:51.96 Gate.setIR 0,5000,0,6 @09:16:52.07 Gate.power :cartridge,:OFF @09:16:52.68 Mounted archive Cartridge @09:16:52.78 Vacuuming Solid Top cartridge 29 for 30 seconds @09:16:52.93 Toroid.in :hold @09:16:53.01 Toroid.seek :cartridge @09:16:54.89 Toroid.in :default @09:16:55.00 Toroid.seek :clear @09:16:56.85 delay 30 seconds @09:17:26.97 Toroid.in :hold @09:17:27.07 Toroid.seek :bypass @09:17:28.75 Toroid.in :default @09:17:28.86 Toroid.seek :clear @09:17:30.72 Carousel.in :default @09:17:30.89 Carousel.home.seek 29 @09:17:32.01 Air.in :unlocking @09:17:32.21 Air.seek :clear @09:17:33.26 Lysis1.in :unlocking @09:17:33.42 Lysis1.seek :clear @09:17:33.57 Plunge.in :unlocking @09:17:33.68 Plunge.seek :clear @09:17:34.84 Carousel.seek 30 @09:17:35.42 Lysis1.in :fast @09:17:35.52 Lysis1.seek :locked @09:17:38.18 Plunge.in :default @09:17:38.34 Plunge.seek :locked @09:17:38.50 Air.in :fast @09:17:38.57 Air.seek :locked @09:17:39.59 Carousel.reconfigure :locked @09:17:39.79 Powering cartridge bus @09:17:42.50 Gate.setIR 1750,5000,0,6 @09:17:42.63 Gate.setIR 0,5000,0,6 @09:17:42.74 PuckHeater.configure :default #ambient 22.3C @09:17:42.87 Gate.power :heat,:ON,limit to 5000mA @09:17:43.57 Mounted lysis Cartridge board#Q05064 @09:17:43.65 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:17:43.70 Vacuuming Vent Top cartridge 30 for 30 seconds @09:17:43.75 Air.in :default @09:17:43.85 Air.seek :evac @09:17:58.48 Toroid.in :hold @09:17:58.59 Toroid.seek :cartridge @09:18:00.48 Toroid.in :default @09:18:00.59 Toroid.seek :clear @09:18:02.43 Polling Can every 2:00 @09:18:02.99 PuckHeater.configure :default #ambient 22.2C @09:18:03.10 PuckHeater.seek 41.1C,1:00 @09:18:25.79 PuckHeater.hold 3 seconds,41.1C @09:18:28.93 delayUntil "09:18:31.61" @09:18:31.73 Toroid.in :hold @09:18:31.93 Toroid.seek :bypass @09:18:33.62 Toroid.in :default @09:18:33.72 Toroid.seek :clear @09:18:35.38 delay 2 seconds @09:18:37.41 Air.seek :full @09:18:52.08 Gate.power :heat,:OFF @09:18:52.10 Gate.power :cartridge,:OFF @09:18:52.25 Carousel.in :default @09:18:52.44 Carousel.home.seek 30 @09:18:52.92 Air.in :unlocking @09:18:53.08 Air.seek :clear @09:18:54.13 Lysis1.in :unlocking @09:18:54.30 Lysis1.seek :clear @09:18:54.50 Plunge.in :unlocking @09:18:54.60 Plunge.seek :clear @09:18:55.75 Carousel.seek 31 @09:18:56.38 Lysis1.in :fast @09:18:56.50 Lysis1.seek :locked @09:18:59.15 Plunge.in :default @09:18:59.25 Plunge.seek :locked @09:18:59.47 Air.in :fast @09:18:59.57 Air.seek :locked @09:19:00.58 Carousel.reconfigure :locked @09:19:00.78 Powering cartridge bus @09:19:03.45 Gate.setIR 1750,5000,0,6 @09:19:03.59 Gate.setIR 0,5000,0,6 @09:19:03.70 Gate.power :cartridge,:OFF @09:19:04.29 Mounted archive Cartridge @09:19:04.43 Vacuuming Solid Top cartridge 31 for 30 seconds @09:19:04.58 Toroid.in :hold @09:19:04.69 Toroid.seek :cartridge @09:19:06.57 Toroid.in :default @09:19:06.67 Toroid.seek :clear @09:19:08.52 delay 30 seconds @09:19:38.65 Toroid.in :hold @09:19:38.75 Toroid.seek :bypass @09:19:40.44 Toroid.in :default @09:19:40.56 Toroid.seek :clear @09:19:42.46 Carousel.in :default @09:19:42.63 Carousel.home.seek 31 @09:19:43.75 Air.in :unlocking @09:19:43.93 Air.seek :clear @09:19:44.99 Lysis1.in :unlocking @09:19:45.15 Lysis1.seek :clear @09:19:45.31 Plunge.in :unlocking @09:19:45.41 Plunge.seek :clear @09:19:46.58 Carousel.seek 32 @09:19:47.18 Lysis1.in :fast @09:19:47.28 Lysis1.seek :locked @09:19:49.94 Plunge.in :default @09:19:50.07 Plunge.seek :locked @09:19:50.24 Air.in :fast @09:19:50.35 Air.seek :locked @09:19:51.39 Carousel.reconfigure :locked @09:19:51.58 Powering cartridge bus @09:19:54.25 Gate.setIR 1750,5000,0,6 @09:19:54.39 Gate.setIR 0,5000,0,6 @09:19:54.49 PuckHeater.configure :default #ambient 22.2C @09:19:54.59 Gate.power :heat,:ON,limit to 5000mA @09:19:55.31 Mounted lysis Cartridge board#Q05034 @09:19:55.40 Using this cartridge's heat loss curve for HeatingAccelInCarousel @09:19:55.45 Vacuuming Vent Top cartridge 32 for 30 seconds @09:19:55.50 Air.in :default @09:19:55.61 Air.seek :evac @09:20:10.19 Toroid.in :hold @09:20:10.30 Toroid.seek :cartridge @09:20:12.20 Toroid.in :default @09:20:12.32 Toroid.seek :clear @09:20:14.20 Polling Can every 2:00 @09:20:14.76 PuckHeater.configure :default #ambient 22.1C @09:20:14.86 PuckHeater.seek 41.3C,1:00 @09:20:38.17 PuckHeater.hold 3 seconds,41.3C @09:20:41.35 delayUntil "09:20:43.31" @09:20:43.42 Toroid.in :hold @09:20:43.53 Toroid.seek :bypass @09:20:45.22 Toroid.in :default @09:20:45.32 Toroid.seek :clear @09:20:46.98 delay 2 seconds @09:20:49.01 Air.seek :full @09:21:03.67 Gate.power :heat,:OFF @09:21:03.70 Gate.power :cartridge,:OFF @09:21:03.85 Carousel.in :default @09:21:04.01 Carousel.home.seek 32 @09:21:04.51 Air.in :unlocking @09:21:04.69 Air.seek :clear @09:21:05.74 Lysis1.in :unlocking @09:21:05.90 Lysis1.seek :clear @09:21:06.05 Plunge.in :unlocking @09:21:06.16 Plunge.seek :clear @09:21:07.32 Carousel.seek 33 @09:21:07.89 Lysis1.in :fast @09:21:08.00 Lysis1.seek :locked @09:21:10.67 Plunge.in :default @09:21:10.81 Plunge.seek :locked @09:21:10.95 Air.in :fast @09:21:11.06 Air.seek :locked @09:21:12.07 Carousel.reconfigure :locked @09:21:12.28 Powering cartridge bus @09:21:14.95 Gate.setIR 1750,5000,0,6 @09:21:15.08 Gate.setIR 0,5000,0,6 @09:21:15.20 Gate.power :cartridge,:OFF @09:21:15.78 Mounted archive Cartridge @09:21:15.89 Vacuuming Solid Top cartridge 33 for 30 seconds @09:21:16.03 Toroid.in :hold @09:21:16.14 Toroid.seek :cartridge @09:21:18.01 Toroid.in :default @09:21:18.11 Toroid.seek :clear @09:21:19.96 delay 30 seconds @09:21:50.08 Toroid.in :hold @09:21:50.19 Toroid.seek :bypass @09:21:51.86 Toroid.in :default @09:21:51.97 Toroid.seek :clear @09:21:53.85 Carousel.in :default @09:21:54.60 Carousel.home.seek 33 @09:21:55.17 Air.in :unlocking @09:21:55.29 Air.seek :clear @09:21:56.42 Lysis1.in :unlocking @09:21:56.59 Lysis1.seek :clear @09:21:56.76 Plunge.in :unlocking @09:21:56.86 Plunge.seek :clear @09:21:58.02 Carousel.seek 34 @09:21:58.61 Lysis1.in :fast @09:21:58.72 Lysis1.seek :locked @09:22:01.39 Plunge.in :default @09:22:01.52 Plunge.seek :locked @09:22:01.71 Air.in :fast @09:22:01.81 Air.seek :locked @09:22:02.83 Carousel.reconfigure :locked @09:22:03.02 Powering cartridge bus @09:22:05.69 Gate.setIR 1750,5000,0,6 @09:22:05.83 Gate.setIR 0,5000,0,6 @09:22:05.94 PuckHeater.configure :default #ambient 22.1C @09:22:06.06 Gate.power :heat,:ON,limit to 5000mA @09:22:06.75 Mounted lysis Cartridge board#Q05052 @09:22:06.83 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:22:06.89 Vacuuming Vent Top cartridge 34 for 30 seconds @09:22:06.94 Air.in :default @09:22:07.06 Air.seek :evac @09:22:21.67 Toroid.in :hold @09:22:21.77 Toroid.seek :cartridge @09:22:23.64 Toroid.in :default @09:22:23.75 Toroid.seek :clear @09:22:25.59 Polling Can every 2:00 @09:22:26.15 PuckHeater.configure :default #ambient 22.1C @09:22:26.24 PuckHeater.seek 41.1C,1:00 @09:22:48.55 PuckHeater.hold 3 seconds,41.1C @09:22:51.73 delayUntil "09:22:54.78" @09:22:54.89 Toroid.in :hold @09:22:55.00 Toroid.seek :bypass @09:22:56.69 Toroid.in :default @09:22:56.79 Toroid.seek :clear @09:22:58.44 delay 2 seconds @09:23:00.47 Air.seek :full @09:23:15.13 Gate.power :heat,:OFF @09:23:15.16 Gate.power :cartridge,:OFF @09:23:15.30 Carousel.in :default @09:23:15.46 Carousel.home.seek 34 @09:23:15.94 Air.in :unlocking @09:23:16.08 Air.seek :clear @09:23:17.14 Lysis1.in :unlocking @09:23:17.31 Lysis1.seek :clear @09:23:17.39 Plunge.in :unlocking @09:23:17.56 Plunge.seek :clear @09:23:18.80 Carousel.seek 35 @09:23:19.38 Lysis1.in :fast @09:23:19.47 Lysis1.seek :locked @09:23:22.15 Plunge.in :default @09:23:22.29 Plunge.seek :locked @09:23:22.49 Air.in :fast @09:23:22.57 Air.seek :locked @09:23:23.59 Carousel.reconfigure :locked @09:23:23.79 Powering cartridge bus @09:23:26.51 Gate.setIR 1750,5000,0,6 @09:23:26.66 Gate.setIR 0,5000,0,6 @09:23:26.78 Gate.power :cartridge,:OFF @09:23:27.37 Mounted archive Cartridge @09:23:27.47 Vacuuming Solid Top cartridge 35 for 30 seconds @09:23:27.60 Toroid.in :hold @09:23:27.70 Toroid.seek :cartridge @09:23:29.59 Toroid.in :default @09:23:29.69 Toroid.seek :clear @09:23:31.53 delay 30 seconds @09:24:01.65 Toroid.in :hold @09:24:01.76 Toroid.seek :bypass @09:24:03.43 Toroid.in :default @09:24:03.54 Toroid.seek :clear @09:24:05.41 Carousel.in :default @09:24:06.13 Carousel.home.seek 35 @09:24:06.71 Air.in :unlocking @09:24:06.87 Air.seek :clear @09:24:07.93 Lysis1.in :unlocking @09:24:08.11 Lysis1.seek :clear @09:24:08.22 Plunge.in :unlocking @09:24:08.39 Plunge.seek :clear @09:24:09.56 Carousel.seek 36 @09:24:10.19 Lysis1.in :fast @09:24:10.30 Lysis1.seek :locked @09:24:13.01 Plunge.in :default @09:24:13.12 Plunge.seek :locked @09:24:13.31 Air.in :fast @09:24:13.42 Air.seek :locked @09:24:14.43 Carousel.reconfigure :locked @09:24:14.64 Powering cartridge bus @09:24:17.30 Gate.setIR 1750,5000,0,6 @09:24:17.44 Gate.setIR 0,5000,0,6 @09:24:17.54 PuckHeater.configure :default #ambient 22.1C @09:24:17.65 Gate.power :heat,:ON,limit to 5000mA @09:24:18.38 Mounted lysis Cartridge board#Q05066 @09:24:18.45 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:24:18.54 Vacuuming Vent Top cartridge 36 for 30 seconds @09:24:18.59 Air.in :default @09:24:18.71 Air.seek :evac @09:24:33.30 Toroid.in :hold @09:24:33.41 Toroid.seek :cartridge @09:24:35.28 Toroid.in :default @09:24:35.39 Toroid.seek :clear @09:24:37.23 Polling Can every 2:00 @09:24:37.78 PuckHeater.configure :default #ambient 22.1C @09:24:37.88 PuckHeater.seek 41.1C,1:00 @09:25:00.11 PuckHeater.hold 3 seconds,41.1C @09:25:03.34 delayUntil "09:25:06.43" @09:25:06.53 Toroid.in :hold @09:25:06.67 Toroid.seek :bypass @09:25:08.41 Toroid.in :default @09:25:08.51 Toroid.seek :clear @09:25:10.17 delay 2 seconds @09:25:12.20 Air.seek :full @09:25:26.86 Gate.power :heat,:OFF @09:25:26.89 Gate.power :cartridge,:OFF @09:25:27.03 Carousel.in :default @09:25:27.18 Carousel.home.seek 36 @09:25:27.65 Air.in :unlocking @09:25:27.85 Air.seek :clear @09:25:28.91 Lysis1.in :unlocking @09:25:29.10 Lysis1.seek :clear @09:25:29.16 Plunge.in :unlocking @09:25:29.36 Plunge.seek :clear @09:25:30.60 Carousel.seek 37 @09:25:31.19 Lysis1.in :fast @09:25:31.29 Lysis1.seek :locked @09:25:33.96 Plunge.in :default @09:25:34.05 Plunge.seek :locked @09:25:34.25 Air.in :fast @09:25:34.37 Air.seek :locked @09:25:35.40 Carousel.reconfigure :locked @09:25:35.60 Powering cartridge bus @09:25:38.27 Gate.setIR 1750,5000,0,6 @09:25:38.40 Gate.setIR 0,5000,0,6 @09:25:38.52 Gate.power :cartridge,:OFF @09:25:39.11 Mounted archive Cartridge @09:25:39.31 Vacuuming Solid Top cartridge 37 for 30 seconds @09:25:39.59 Toroid.in :hold @09:25:39.74 Toroid.seek :cartridge @09:25:41.64 Toroid.in :default @09:25:41.76 Toroid.seek :clear @09:25:43.61 delay 30 seconds @09:26:13.72 Toroid.in :hold @09:26:13.88 Toroid.seek :bypass @09:26:15.55 Toroid.in :default @09:26:15.65 Toroid.seek :clear @09:26:17.52 Carousel.in :default @09:26:18.30 Carousel.home.seek 37 @09:26:18.83 Air.in :unlocking @09:26:18.98 Air.seek :clear @09:26:20.00 Lysis1.in :unlocking @09:26:20.16 Lysis1.seek :clear @09:26:20.31 Plunge.in :unlocking @09:26:20.47 Plunge.seek :clear @09:26:21.62 Carousel.seek 38 @09:26:22.20 Lysis1.in :fast @09:26:22.31 Lysis1.seek :locked @09:26:25.01 Plunge.in :default @09:26:25.15 Plunge.seek :locked @09:26:25.31 Air.in :fast @09:26:25.42 Air.seek :locked @09:26:26.42 Carousel.reconfigure :locked @09:26:26.64 Powering cartridge bus @09:26:29.31 Gate.setIR 1750,5000,0,6 @09:26:29.44 Gate.setIR 0,5000,0,6 @09:26:29.55 PuckHeater.configure :default #ambient 22.1C @09:26:29.66 Gate.power :heat,:ON,limit to 5000mA @09:26:30.45 Mounted lysis Cartridge board#Q05044 @09:26:30.54 Using this cartridge's heat loss curve for HeatingAccelInCarousel @09:26:30.60 Vacuuming Vent Top cartridge 38 for 30 seconds @09:26:30.63 Air.in :default @09:26:30.75 Air.seek :evac @09:26:45.34 Toroid.in :hold @09:26:45.44 Toroid.seek :cartridge @09:26:47.32 Toroid.in :default @09:26:47.42 Toroid.seek :clear @09:26:49.27 Polling Can every 2:00 @09:26:49.83 PuckHeater.configure :default #ambient 22.1C @09:26:49.92 PuckHeater.seek 41.1C,1:00 @09:27:12.20 PuckHeater.hold 3 seconds,41.1C @09:27:15.43 delayUntil "09:27:18.46" @09:27:18.56 Toroid.in :hold @09:27:18.67 Toroid.seek :bypass @09:27:20.34 Toroid.in :default @09:27:20.49 Toroid.seek :clear @09:27:22.15 delay 2 seconds @09:27:24.18 Air.seek :full @09:27:38.83 Gate.power :heat,:OFF @09:27:38.85 Gate.power :cartridge,:OFF @09:27:39.00 Carousel.in :default @09:27:39.15 Carousel.home.seek 38 @09:27:39.62 Air.in :unlocking @09:27:39.80 Air.seek :clear @09:27:40.85 Lysis1.in :unlocking @09:27:41.00 Lysis1.seek :clear @09:27:41.15 Plunge.in :unlocking @09:27:41.27 Plunge.seek :clear @09:27:42.45 Carousel.seek 39 @09:27:43.03 Lysis1.in :fast @09:27:43.13 Lysis1.seek :locked @09:27:45.79 Plunge.in :default @09:27:45.95 Plunge.seek :locked @09:27:46.08 Air.in :fast @09:27:46.19 Air.seek :locked @09:27:47.20 Carousel.reconfigure :locked @09:27:47.40 Powering cartridge bus @09:27:50.07 Gate.setIR 1750,5000,0,6 @09:27:50.20 Gate.setIR 0,5000,0,6 @09:27:50.32 Gate.power :cartridge,:OFF @09:27:50.95 Mounted archive Cartridge @09:27:51.05 Vacuuming Solid Top cartridge 39 for 30 seconds @09:27:51.19 Toroid.in :hold @09:27:51.28 Toroid.seek :cartridge @09:27:53.16 Toroid.in :default @09:27:53.27 Toroid.seek :clear @09:27:55.11 delay 30 seconds @09:28:25.23 Toroid.in :hold @09:28:25.38 Toroid.seek :bypass @09:28:27.06 Toroid.in :default @09:28:27.16 Toroid.seek :clear @09:28:29.58 Carousel.in :default @09:28:29.84 Carousel.home.seek 39 @09:28:30.32 Air.in :unlocking @09:28:30.54 Air.seek :clear @09:28:31.62 Lysis1.in :unlocking @09:28:31.81 Lysis1.seek :clear @09:28:31.87 Plunge.in :unlocking @09:28:32.05 Plunge.seek :clear @09:28:33.27 Carousel.seek 40 @09:28:33.86 Lysis1.in :fast @09:28:33.97 Lysis1.seek :locked @09:28:36.64 Plunge.in :default @09:28:36.78 Plunge.seek :locked @09:28:36.95 Air.in :fast @09:28:37.04 Air.seek :locked @09:28:38.06 Carousel.reconfigure :locked @09:28:38.26 Powering cartridge bus @09:28:40.93 Gate.setIR 1750,5000,0,6 @09:28:41.06 Gate.setIR 0,5000,0,6 @09:28:41.17 PuckHeater.configure :default #ambient 22.1C @09:28:41.29 Gate.power :heat,:ON,limit to 5000mA @09:28:41.99 Mounted lysis Cartridge board#Q05041 @09:28:42.07 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:28:42.13 Vacuuming Vent Top cartridge 40 for 30 seconds @09:28:42.17 Air.in :default @09:28:42.28 Air.seek :evac @09:28:56.91 Toroid.in :hold @09:28:57.00 Toroid.seek :cartridge @09:28:58.86 Toroid.in :default @09:28:58.96 Toroid.seek :clear @09:29:00.81 Polling Can every 2:00 @09:29:01.36 PuckHeater.configure :default #ambient 22.1C @09:29:01.47 PuckHeater.seek 41.1C,1:00 @09:29:23.71 PuckHeater.hold 3 seconds,41.1C @09:29:26.94 delayUntil "09:29:30.02" @09:29:30.13 Toroid.in :hold @09:29:30.24 Toroid.seek :bypass @09:29:31.92 Toroid.in :default @09:29:32.03 Toroid.seek :clear @09:29:33.68 delay 2 seconds @09:29:35.71 Air.seek :full @09:29:50.41 Gate.power :heat,:OFF @09:29:50.44 Gate.power :cartridge,:OFF @09:29:50.58 Carousel.in :default @09:29:50.74 Carousel.home.seek 40 @09:29:51.20 Air.in :unlocking @09:29:51.37 Air.seek :clear @09:29:52.46 Lysis1.in :unlocking @09:29:52.65 Lysis1.seek :clear @09:29:52.77 Plunge.in :unlocking @09:29:52.90 Plunge.seek :clear @09:29:54.10 Carousel.seek 41 @09:29:54.68 Lysis1.in :fast @09:29:54.78 Lysis1.seek :locked @09:29:57.46 Plunge.in :default @09:29:57.58 Plunge.seek :locked @09:29:57.76 Air.in :fast @09:29:57.86 Air.seek :locked @09:29:58.87 Carousel.reconfigure :locked @09:29:59.07 Powering cartridge bus @09:30:01.74 Gate.setIR 1750,5000,0,6 @09:30:01.87 Gate.setIR 0,5000,0,6 @09:30:02.00 Gate.power :cartridge,:OFF @09:30:02.60 Mounted archive Cartridge @09:30:02.71 Vacuuming Solid Top cartridge 41 for 30 seconds @09:30:02.85 Toroid.in :hold @09:30:02.94 Toroid.seek :cartridge @09:30:04.82 Toroid.in :default @09:30:04.92 Toroid.seek :clear @09:30:06.76 delay 30 seconds @09:30:36.88 Toroid.in :hold @09:30:37.04 Toroid.seek :bypass @09:30:38.71 Toroid.in :default @09:30:38.81 Toroid.seek :clear @09:30:41.23 Carousel.in :default @09:30:41.50 Carousel.home.seek 41 @09:30:42.01 Air.in :unlocking @09:30:42.17 Air.seek :clear @09:30:43.22 Lysis1.in :unlocking @09:30:43.39 Lysis1.seek :clear @09:30:43.50 Plunge.in :unlocking @09:30:43.64 Plunge.seek :clear @09:30:44.83 Carousel.seek 42 @09:30:45.41 Lysis1.in :fast @09:30:45.51 Lysis1.seek :locked @09:30:48.17 Plunge.in :default @09:30:48.31 Plunge.seek :locked @09:30:48.49 Air.in :fast @09:30:48.58 Air.seek :locked @09:30:49.60 Carousel.reconfigure :locked @09:30:49.80 Powering cartridge bus @09:30:52.51 Gate.setIR 1750,5000,0,6 @09:30:52.64 Gate.setIR 0,5000,0,6 @09:30:52.75 PuckHeater.configure :default #ambient 22.1C @09:30:52.87 Gate.power :heat,:ON,limit to 5000mA @09:30:53.61 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @09:30:53.69 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:30:53.74 Vacuuming Vent Top cartridge 42 for 30 seconds @09:30:53.79 Air.in :default @09:30:53.91 Air.seek :evac @09:31:08.52 Toroid.in :hold @09:31:08.63 Toroid.seek :cartridge @09:31:10.51 Toroid.in :default @09:31:10.63 Toroid.seek :clear @09:31:12.47 Polling Can every 2:00 @09:31:13.03 PuckHeater.configure :default #ambient 22.1C @09:31:13.13 PuckHeater.seek 41.1C,1:00 @09:31:35.36 PuckHeater.hold 3 seconds,41.1C @09:31:38.61 delayUntil "09:31:41.65" @09:31:41.76 Toroid.in :hold @09:31:41.87 Toroid.seek :bypass @09:31:43.54 Toroid.in :default @09:31:43.65 Toroid.seek :clear @09:31:45.31 delay 2 seconds @09:31:47.35 Air.seek :full @09:32:02.00 Gate.power :heat,:OFF @09:32:02.03 Gate.power :cartridge,:OFF @09:32:02.18 Carousel.in :default @09:32:02.35 Carousel.home.seek 42 @09:32:02.86 Air.in :unlocking @09:32:03.06 Air.seek :clear @09:32:04.11 Lysis1.in :unlocking @09:32:04.27 Lysis1.seek :clear @09:32:04.45 Plunge.in :unlocking @09:32:04.56 Plunge.seek :clear @09:32:05.70 Carousel.seek 43 @09:32:06.29 Lysis1.in :fast @09:32:06.44 Lysis1.seek :locked @09:32:09.11 Plunge.in :default @09:32:09.21 Plunge.seek :locked @09:32:09.42 Air.in :fast @09:32:09.52 Air.seek :locked @09:32:10.53 Carousel.reconfigure :locked @09:32:10.73 Powering cartridge bus @09:32:13.40 Gate.setIR 1750,5000,0,6 @09:32:13.54 Gate.setIR 0,5000,0,6 @09:32:13.66 Gate.power :cartridge,:OFF @09:32:14.25 Mounted archive Cartridge @09:32:14.39 Vacuuming Solid Top cartridge 43 for 30 seconds @09:32:14.54 Toroid.in :hold @09:32:14.63 Toroid.seek :cartridge @09:32:16.51 Toroid.in :default @09:32:16.61 Toroid.seek :clear @09:32:18.46 delay 30 seconds @09:32:48.58 Toroid.in :hold @09:32:48.75 Toroid.seek :bypass @09:32:50.48 Toroid.in :default @09:32:50.58 Toroid.seek :clear @09:32:53.03 Carousel.in :default @09:32:53.30 Carousel.home.seek 43 @09:32:53.80 Air.in :unlocking @09:32:54.00 Air.seek :clear @09:32:55.08 Lysis1.in :unlocking @09:32:55.28 Lysis1.seek :clear @09:32:55.34 Plunge.in :unlocking @09:32:55.51 Plunge.seek :clear @09:32:56.74 Carousel.seek 44 @09:32:57.34 Lysis1.in :fast @09:32:57.44 Lysis1.seek :locked @09:33:00.10 Plunge.in :default @09:33:00.21 Plunge.seek :locked @09:33:00.44 Air.in :fast @09:33:00.52 Air.seek :locked @09:33:01.54 Carousel.reconfigure :locked @09:33:01.74 Powering cartridge bus @09:33:04.43 Gate.setIR 1750,5000,0,6 @09:33:04.57 Gate.setIR 0,5000,0,6 @09:33:04.68 PuckHeater.configure :default #ambient 22.1C @09:33:04.79 Gate.power :heat,:ON,limit to 5000mA @09:33:05.50 Mounted lysis Cartridge board#Q05065 @09:33:05.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:33:05.65 Vacuuming Vent Top cartridge 44 for 30 seconds @09:33:05.69 Air.in :default @09:33:05.83 Air.seek :evac @09:33:20.43 Toroid.in :hold @09:33:20.54 Toroid.seek :cartridge @09:33:22.44 Toroid.in :default @09:33:22.54 Toroid.seek :clear @09:33:24.41 Polling Can every 2:00 @09:33:24.97 PuckHeater.configure :default #ambient 22.1C @09:33:25.08 PuckHeater.seek 41.1C,1:00 @09:33:47.32 PuckHeater.hold 3 seconds,41.1C @09:33:50.52 delayUntil "09:33:53.56" @09:33:53.67 Toroid.in :hold @09:33:53.77 Toroid.seek :bypass @09:33:55.45 Toroid.in :default @09:33:55.55 Toroid.seek :clear @09:33:57.21 delay 2 seconds @09:33:59.24 Air.seek :full @09:34:13.90 Gate.power :heat,:OFF @09:34:13.93 Gate.power :cartridge,:OFF @09:34:14.07 Carousel.in :default @09:34:14.23 Carousel.home.seek 44 @09:34:14.71 Air.in :unlocking @09:34:14.89 Air.seek :clear @09:34:15.95 Lysis1.in :unlocking @09:34:16.11 Lysis1.seek :clear @09:34:16.28 Plunge.in :unlocking @09:34:16.42 Plunge.seek :clear @09:34:17.57 Carousel.seek 45 @09:34:18.16 Lysis1.in :fast @09:34:18.25 Lysis1.seek :locked @09:34:20.92 Plunge.in :default @09:34:21.07 Plunge.seek :locked @09:34:21.21 Air.in :fast @09:34:21.32 Air.seek :locked @09:34:22.33 Carousel.reconfigure :locked @09:34:22.57 Powering cartridge bus @09:34:25.24 Gate.setIR 1750,5000,0,6 @09:34:25.37 Gate.setIR 0,5000,0,6 @09:34:25.49 Gate.power :cartridge,:OFF @09:34:26.09 Mounted archive Cartridge @09:34:26.18 Vacuuming Solid Top cartridge 45 for 30 seconds @09:34:26.31 Toroid.in :hold @09:34:26.46 Toroid.seek :cartridge @09:34:28.36 Toroid.in :default @09:34:28.48 Toroid.seek :clear @09:34:30.33 delay 30 seconds @09:35:00.47 Toroid.in :hold @09:35:00.63 Toroid.seek :bypass @09:35:02.31 Toroid.in :default @09:35:02.46 Toroid.seek :clear @09:35:04.32 Carousel.in :default @09:35:04.61 Carousel.home.seek 45 @09:35:05.07 Air.in :unlocking @09:35:05.24 Air.seek :clear @09:35:06.30 Lysis1.in :unlocking @09:35:06.48 Lysis1.seek :clear @09:35:06.66 Plunge.in :unlocking @09:35:06.77 Plunge.seek :clear @09:35:07.92 Carousel.seek 46 @09:35:08.55 Lysis1.in :fast @09:35:08.66 Lysis1.seek :locked @09:35:11.33 Plunge.in :default @09:35:11.45 Plunge.seek :locked @09:35:11.64 Air.in :fast @09:35:11.75 Air.seek :locked @09:35:12.76 Carousel.reconfigure :locked @09:35:12.98 Powering cartridge bus @09:35:15.65 Gate.setIR 1750,5000,0,6 @09:35:15.78 Gate.setIR 0,5000,0,6 @09:35:15.89 PuckHeater.configure :default #ambient 22.1C @09:35:16.00 Gate.power :heat,:ON,limit to 5000mA @09:35:16.71 Mounted lysis Cartridge board#Q05046 @09:35:16.79 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:35:16.84 Vacuuming Vent Top cartridge 46 for 30 seconds @09:35:16.89 Air.in :default @09:35:17.01 Air.seek :evac @09:35:31.61 Toroid.in :hold @09:35:31.72 Toroid.seek :cartridge @09:35:33.58 Toroid.in :default @09:35:33.69 Toroid.seek :clear @09:35:35.51 Polling Can every 2:00 @09:35:36.07 PuckHeater.configure :default #ambient 22.1C @09:35:36.17 PuckHeater.seek 41.1C,1:00 @09:35:59.49 PuckHeater.hold 3 seconds,41.1C @09:36:02.74 delayUntil "09:36:04.74" @09:36:04.84 Toroid.in :hold @09:36:04.95 Toroid.seek :bypass @09:36:06.64 Toroid.in :default @09:36:06.75 Toroid.seek :clear @09:36:08.42 delay 2 seconds @09:36:10.45 Air.seek :full @09:36:25.11 Gate.power :heat,:OFF @09:36:25.14 Gate.power :cartridge,:OFF @09:36:25.28 Carousel.in :default @09:36:25.43 Carousel.home.seek 46 @09:36:25.90 Air.in :unlocking @09:36:26.07 Air.seek :clear @09:36:27.11 Lysis1.in :unlocking @09:36:27.26 Lysis1.seek :clear @09:36:27.44 Plunge.in :unlocking @09:36:27.54 Plunge.seek :clear @09:36:28.70 Carousel.seek 47 @09:36:29.28 Lysis1.in :fast @09:36:29.39 Lysis1.seek :locked @09:36:32.05 Plunge.in :default @09:36:32.14 Plunge.seek :locked @09:36:32.37 Air.in :fast @09:36:32.47 Air.seek :locked @09:36:33.48 Carousel.reconfigure :locked @09:36:33.68 Powering cartridge bus @09:36:36.38 Gate.setIR 1750,5000,0,6 @09:36:36.52 Gate.setIR 0,5000,0,6 @09:36:36.64 Gate.power :cartridge,:OFF @09:36:37.23 Mounted archive Cartridge @09:36:37.33 Vacuuming Solid Top cartridge 47 for 30 seconds @09:36:37.47 Toroid.in :hold @09:36:37.55 Toroid.seek :cartridge @09:36:39.44 Toroid.in :default @09:36:39.54 Toroid.seek :clear @09:36:41.40 delay 30 seconds @09:37:11.52 Toroid.in :hold @09:37:11.67 Toroid.seek :bypass @09:37:13.35 Toroid.in :default @09:37:13.99 Toroid.seek :clear @09:37:15.97 Carousel.in :default @09:37:16.14 Carousel.home.seek 47 @09:37:16.65 Air.in :unlocking @09:37:16.85 Air.seek :clear @09:37:17.90 Lysis1.in :unlocking @09:37:18.05 Lysis1.seek :clear @09:37:18.20 Plunge.in :unlocking @09:37:18.32 Plunge.seek :clear @09:37:19.52 Carousel.seek 48 @09:37:20.11 Lysis1.in :fast @09:37:20.21 Lysis1.seek :locked @09:37:22.89 Plunge.in :default @09:37:23.04 Plunge.seek :locked @09:37:23.20 Air.in :fast @09:37:23.30 Air.seek :locked @09:37:24.31 Carousel.reconfigure :locked @09:37:24.55 Powering cartridge bus @09:37:27.22 Gate.setIR 1750,5000,0,6 @09:37:27.36 Gate.setIR 0,5000,0,6 @09:37:27.47 PuckHeater.configure :default #ambient 22.1C @09:37:27.58 Gate.power :heat,:ON,limit to 5000mA @09:37:28.29 Mounted lysis Cartridge board#Q05062 @09:37:28.40 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:37:28.46 Vacuuming Vent Top cartridge 48 for 30 seconds @09:37:28.50 Air.in :default @09:37:28.61 Air.seek :evac @09:37:43.20 Toroid.in :hold @09:37:43.31 Toroid.seek :cartridge @09:37:45.19 Toroid.in :default @09:37:45.29 Toroid.seek :clear @09:37:47.14 Polling Can every 2:00 @09:37:47.70 PuckHeater.configure :default #ambient 22.1C @09:37:47.79 PuckHeater.seek 41.1C,1:00 @09:38:10.07 PuckHeater.hold 3 seconds,41.1C @09:38:13.28 delayUntil "09:38:16.33" @09:38:16.46 Toroid.in :hold @09:38:16.57 Toroid.seek :bypass @09:38:18.25 Toroid.in :default @09:38:18.39 Toroid.seek :clear @09:38:20.04 delay 2 seconds @09:38:22.07 Air.seek :full @09:38:36.72 Gate.power :heat,:OFF @09:38:36.74 Gate.power :cartridge,:OFF @09:38:36.90 Carousel.in :default @09:38:37.05 Carousel.home.seek 48 @09:38:37.52 Air.in :unlocking @09:38:37.71 Air.seek :clear @09:38:38.76 Lysis1.in :unlocking @09:38:38.91 Lysis1.seek :clear @09:38:39.07 Plunge.in :unlocking @09:38:39.18 Plunge.seek :clear @09:38:40.33 Carousel.seek 49 @09:38:40.95 Lysis1.in :fast @09:38:41.05 Lysis1.seek :locked @09:38:43.73 Plunge.in :default @09:38:43.85 Plunge.seek :locked @09:38:44.03 Air.in :fast @09:38:44.12 Air.seek :locked @09:38:45.14 Carousel.reconfigure :locked @09:38:45.34 Powering cartridge bus @09:38:48.02 Gate.setIR 1750,5000,0,6 @09:38:48.15 Gate.setIR 0,5000,0,6 @09:38:48.27 Gate.power :cartridge,:OFF @09:38:48.87 Mounted archive Cartridge @09:38:48.96 Vacuuming Solid Top cartridge 49 for 30 seconds @09:38:49.10 Toroid.in :hold @09:38:49.20 Toroid.seek :cartridge @09:38:51.07 Toroid.in :default @09:38:51.18 Toroid.seek :clear @09:38:53.02 delay 30 seconds @09:39:23.14 Toroid.in :hold @09:39:23.29 Toroid.seek :bypass @09:39:25.51 Toroid.in :default @09:39:25.72 Toroid.seek :clear @09:39:27.59 Carousel.in :default @09:39:27.76 Carousel.home.seek 49 @09:39:28.24 Air.in :unlocking @09:39:28.41 Air.seek :clear @09:39:29.49 Lysis1.in :unlocking @09:39:29.64 Lysis1.seek :clear @09:39:29.79 Plunge.in :unlocking @09:39:29.90 Plunge.seek :clear @09:39:31.06 Carousel.seek 50 @09:39:31.65 Lysis1.in :fast @09:39:31.76 Lysis1.seek :locked @09:39:34.47 Plunge.in :default @09:39:34.58 Plunge.seek :locked @09:39:34.76 Air.in :fast @09:39:34.86 Air.seek :locked @09:39:35.87 Carousel.reconfigure :locked @09:39:36.09 Powering cartridge bus @09:39:38.79 Gate.setIR 1750,5000,0,6 @09:39:38.93 Gate.setIR 0,5000,0,6 @09:39:39.04 PuckHeater.configure :default #ambient 22.1C @09:39:39.16 Gate.power :heat,:ON,limit to 5000mA @09:39:39.87 Mounted lysis Cartridge board#Q05053 @09:39:39.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:39:40.00 Vacuuming Vent Top cartridge 50 for 30 seconds @09:39:40.05 Air.in :default @09:39:40.17 Air.seek :evac @09:39:54.76 Toroid.in :hold @09:39:54.87 Toroid.seek :cartridge @09:39:56.74 Toroid.in :default @09:39:56.85 Toroid.seek :clear @09:39:58.70 Polling Can every 2:00 @09:39:59.25 PuckHeater.configure :default #ambient 22.1C @09:39:59.35 PuckHeater.seek 41.1C,1:00 @09:40:22.66 PuckHeater.hold 3 seconds,41.1C @09:40:25.83 delayUntil "09:40:27.89" @09:40:28.01 Toroid.in :hold @09:40:28.12 Toroid.seek :bypass @09:40:29.80 Toroid.in :default @09:40:29.91 Toroid.seek :clear @09:40:31.56 delay 2 seconds @09:40:33.59 Air.seek :full @09:40:48.25 Gate.power :heat,:OFF @09:40:48.27 Gate.power :cartridge,:OFF @09:40:48.45 Carousel.in :default @09:40:48.60 Carousel.home.seek 50 @09:40:49.06 Air.in :unlocking @09:40:49.25 Air.seek :clear @09:40:50.31 Lysis1.in :unlocking @09:40:50.53 Lysis1.seek :clear @09:40:50.65 Plunge.in :unlocking @09:40:50.78 Plunge.seek :clear @09:40:51.96 Carousel.seek 51 @09:40:52.56 Lysis1.in :fast @09:40:52.64 Lysis1.seek :locked @09:40:55.31 Plunge.in :default @09:40:55.46 Plunge.seek :locked @09:40:55.61 Air.in :fast @09:40:55.71 Air.seek :locked @09:40:56.72 Carousel.reconfigure :locked @09:40:56.94 Powering cartridge bus @09:40:59.60 Gate.setIR 1750,5000,0,6 @09:40:59.74 Gate.setIR 0,5000,0,6 @09:40:59.85 Gate.power :cartridge,:OFF @09:41:00.44 Mounted archive Cartridge @09:41:00.55 Vacuuming Solid Top cartridge 51 for 30 seconds @09:41:00.70 Toroid.in :hold @09:41:00.79 Toroid.seek :cartridge @09:41:02.68 Toroid.in :default @09:41:02.78 Toroid.seek :clear @09:41:04.63 delay 30 seconds @09:41:34.75 Toroid.in :hold @09:41:34.90 Toroid.seek :bypass @09:41:37.30 Toroid.in :default @09:41:37.39 Toroid.seek :clear @09:41:39.27 Carousel.in :default @09:41:39.44 Carousel.home.seek 51 @09:41:39.93 Air.in :unlocking @09:41:40.11 Air.seek :clear @09:41:41.16 Lysis1.in :unlocking @09:41:41.30 Lysis1.seek :clear @09:41:41.52 Plunge.in :unlocking @09:41:41.63 Plunge.seek :clear @09:41:42.78 Carousel.seek 56 @09:41:44.19 Lysis1.in :fast @09:41:44.29 Lysis1.seek :locked @09:41:46.99 Plunge.in :default @09:41:47.08 Plunge.seek :locked @09:41:47.29 Air.in :fast @09:41:47.39 Air.seek :locked @09:41:48.41 Carousel.reconfigure :locked @09:41:48.61 Powering cartridge bus @09:41:51.28 Gate.setIR 1750,5000,0,6 @09:41:51.42 Gate.setIR 0,5000,0,6 @09:41:51.53 Gate.power :cartridge,:OFF @09:41:52.12 Mounted archive Cartridge @09:41:52.21 Vacuuming Solid Top cartridge 56 for 30 seconds @09:41:52.38 Toroid.in :hold @09:41:52.48 Toroid.seek :cartridge @09:41:54.37 Toroid.in :default @09:41:54.49 Toroid.seek :clear @09:41:56.36 delay 30 seconds @09:42:26.49 Toroid.in :hold @09:42:26.60 Toroid.seek :bypass @09:42:28.28 Toroid.in :default @09:42:28.41 Toroid.seek :clear @09:42:30.28 Carousel.in :default @09:42:30.48 Carousel.home.seek 56 @09:42:30.95 Air.in :unlocking @09:42:31.10 Air.seek :clear @09:42:32.84 Lysis1.in :unlocking @09:42:33.03 Lysis1.seek :clear @09:42:33.10 Plunge.in :unlocking @09:42:33.28 Plunge.seek :clear @09:42:34.50 Carousel.seek 57 @09:42:35.08 Lysis1.in :fast @09:42:35.19 Lysis1.seek :locked @09:42:37.85 Plunge.in :default @09:42:37.99 Plunge.seek :locked @09:42:38.16 Air.in :fast @09:42:38.27 Air.seek :locked @09:42:39.29 Carousel.reconfigure :locked @09:42:39.50 Powering cartridge bus @09:42:42.17 Gate.setIR 1750,5000,0,6 @09:42:42.31 Gate.setIR 0,5000,0,6 @09:42:42.45 PuckHeater.configure :default #ambient 22.1C @09:42:42.56 Gate.power :heat,:ON,limit to 5000mA @09:42:43.25 Mounted lysis Cartridge board#Q05075 @09:42:43.33 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:42:43.39 Vacuuming Vent Top cartridge 57 for 30 seconds @09:42:43.44 Air.in :default @09:42:43.55 Air.seek :evac @09:42:58.14 Toroid.in :hold @09:42:58.24 Toroid.seek :cartridge @09:43:00.13 Toroid.in :default @09:43:00.24 Toroid.seek :clear @09:43:02.08 Polling Can every 2:00 @09:43:02.64 PuckHeater.configure :default #ambient 22.0C @09:43:02.75 PuckHeater.seek 41.1C,1:00 @09:43:25.44 PuckHeater.hold 3 seconds,41.1C @09:43:28.66 delayUntil "09:43:31.26" @09:43:31.37 Toroid.in :hold @09:43:31.47 Toroid.seek :bypass @09:43:33.16 Toroid.in :default @09:43:33.37 Toroid.seek :clear @09:43:35.02 delay 2 seconds @09:43:37.05 Air.seek :full @09:43:51.71 Gate.power :heat,:OFF @09:43:51.73 Gate.power :cartridge,:OFF @09:43:51.89 Carousel.in :default @09:43:52.04 Carousel.home.seek 57 @09:43:52.55 Air.in :unlocking @09:43:52.72 Air.seek :clear @09:43:53.77 Lysis1.in :unlocking @09:43:53.95 Lysis1.seek :clear @09:43:54.02 Plunge.in :unlocking @09:43:54.21 Plunge.seek :clear @09:43:55.44 Carousel.seek 58 @09:43:56.02 Lysis1.in :fast @09:43:56.12 Lysis1.seek :locked @09:43:58.81 Plunge.in :default @09:43:58.93 Plunge.seek :locked @09:43:59.11 Air.in :fast @09:43:59.22 Air.seek :locked @09:44:00.23 Carousel.reconfigure :locked @09:44:00.47 Powering cartridge bus @09:44:03.14 Gate.setIR 1750,5000,0,6 @09:44:03.27 Gate.setIR 0,5000,0,6 @09:44:03.39 Gate.power :cartridge,:OFF @09:44:03.98 Mounted archive Cartridge @09:44:04.08 Vacuuming Solid Top cartridge 58 for 30 seconds @09:44:04.21 Toroid.in :hold @09:44:04.31 Toroid.seek :cartridge @09:44:06.24 Toroid.in :default @09:44:06.37 Toroid.seek :clear @09:44:08.22 delay 30 seconds @09:44:38.34 Toroid.in :hold @09:44:38.48 Toroid.seek :bypass @09:44:40.16 Toroid.in :default @09:44:40.27 Toroid.seek :clear @09:44:42.12 Carousel.in :default @09:44:42.29 Carousel.home.seek 58 @09:44:42.79 Air.in :unlocking @09:44:43.51 Air.seek :clear @09:44:44.66 Lysis1.in :unlocking @09:44:44.84 Lysis1.seek :clear @09:44:44.99 Plunge.in :unlocking @09:44:45.08 Plunge.seek :clear @09:44:46.27 Carousel.seek 59 @09:44:46.90 Lysis1.in :fast @09:44:47.01 Lysis1.seek :locked @09:44:49.68 Plunge.in :default @09:44:49.77 Plunge.seek :locked @09:44:49.97 Air.in :fast @09:44:50.06 Air.seek :locked @09:44:51.07 Carousel.reconfigure :locked @09:44:51.29 Powering cartridge bus @09:44:53.96 Gate.setIR 1750,5000,0,6 @09:44:54.10 Gate.setIR 0,5000,0,6 @09:44:54.21 PuckHeater.configure :default #ambient 22.0C @09:44:54.33 Gate.power :heat,:ON,limit to 5000mA @09:44:55.05 Mounted lysis Cartridge board#Q05086 @09:44:55.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:44:55.19 Vacuuming Vent Top cartridge 59 for 30 seconds @09:44:55.22 Air.in :default @09:44:55.34 Air.seek :evac @09:45:09.93 Toroid.in :hold @09:45:10.04 Toroid.seek :cartridge @09:45:11.92 Toroid.in :default @09:45:12.03 Toroid.seek :clear @09:45:13.88 Polling Can every 2:00 @09:45:14.43 PuckHeater.configure :default #ambient 22.0C @09:45:14.53 PuckHeater.seek 41.1C,1:00 @09:45:37.17 PuckHeater.hold 3 seconds,41.1C @09:45:40.47 delayUntil "09:45:43.06" @09:45:43.16 Toroid.in :hold @09:45:43.81 Toroid.seek :bypass @09:45:45.59 Toroid.in :default @09:45:45.70 Toroid.seek :clear @09:45:47.35 delay 2 seconds @09:45:49.38 Air.seek :full @09:46:04.04 Gate.power :heat,:OFF @09:46:04.06 Gate.power :cartridge,:OFF @09:46:04.21 Carousel.in :default @09:46:04.40 Carousel.home.seek 59 @09:46:04.87 Air.in :unlocking @09:46:05.05 Air.seek :clear @09:46:06.12 Lysis1.in :unlocking @09:46:06.30 Lysis1.seek :clear @09:46:06.42 Plunge.in :unlocking @09:46:06.60 Plunge.seek :clear @09:46:07.83 Carousel.seek 60 @09:46:08.45 Lysis1.in :fast @09:46:08.56 Lysis1.seek :locked @09:46:11.23 Plunge.in :default @09:46:11.39 Plunge.seek :locked @09:46:11.54 Air.in :fast @09:46:11.65 Air.seek :locked @09:46:12.66 Carousel.reconfigure :locked @09:46:12.86 Powering cartridge bus @09:46:15.53 Gate.setIR 1750,5000,0,6 @09:46:15.66 Gate.setIR 0,5000,0,6 @09:46:15.77 PuckHeater.configure :default #ambient 22.0C @09:46:15.89 Gate.power :heat,:ON,limit to 5000mA @09:46:16.61 Mounted lysis Cartridge board#Q05051 @09:46:16.69 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:46:16.74 Vacuuming Vent Top cartridge 60 for 30 seconds @09:46:16.79 Air.in :default @09:46:16.91 Air.seek :evac @09:46:31.52 Toroid.in :hold @09:46:31.63 Toroid.seek :cartridge @09:46:33.50 Toroid.in :default @09:46:33.61 Toroid.seek :clear @09:46:35.45 Polling Can every 2:00 @09:46:36.01 PuckHeater.configure :default #ambient 22.0C @09:46:36.11 PuckHeater.seek 41.1C,1:00 @09:46:58.33 PuckHeater.hold 3 seconds,41.1C @09:47:01.54 delayUntil "09:47:04.65" @09:47:04.75 Toroid.in :hold @09:47:04.86 Toroid.seek :bypass @09:47:06.55 Toroid.in :default @09:47:06.65 Toroid.seek :clear @09:47:08.31 delay 2 seconds @09:47:10.34 Air.seek :full @09:47:24.89 Gate.power :heat,:OFF @09:47:24.92 Gate.power :cartridge,:OFF @09:47:25.06 Carousel.in :default @09:47:25.22 Carousel.home.seek 60 @09:47:25.69 Air.in :unlocking @09:47:25.86 Air.seek :clear @09:47:26.90 Lysis1.in :unlocking @09:47:27.07 Lysis1.seek :clear @09:47:27.22 Plunge.in :unlocking @09:47:27.33 Plunge.seek :clear @09:47:28.48 Cartridges under vacuum. Individual Leak Test begins @09:47:28.52 Waiting for bill to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @09:47:52.72 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @09:47:52.75 delay 10:00 @09:57:52.83 Waiting for bill to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @09:58:37.32 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @09:58:37.35 move through each cartridge position & record end vacuum @09:58:37.40 Vacuuming cartridge 1 @09:58:37.76 Carousel.seek 1 @09:58:50.13 Lysis1.in :fast @09:58:50.24 Lysis1.seek :locked @09:58:52.90 Plunge.in :default @09:58:53.01 Plunge.seek :locked @09:58:53.21 Air.in :fast @09:58:53.31 Air.seek :locked @09:58:54.30 Carousel.reconfigure :locked @09:58:54.53 Powering cartridge bus @09:58:57.21 Gate.setIR 1750,5000,0,6 @09:58:57.34 Gate.setIR 0,5000,0,6 @09:58:57.45 PuckHeater.configure :default #ambient 22.0C @09:58:57.56 Gate.power :heat,:ON,limit to 5000mA @09:58:58.96 Mounted lysis Cartridge board#Q05059 @09:58:59.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:58:59.20 Toroid.in :hold @09:58:59.29 Toroid.seek :cartridge @09:59:01.16 Toroid.in :default @09:59:01.27 Toroid.seek :clear @09:59:03.12 Waiting for bill to answer "record Vacuum" @09:59:12.34 Answered record Vacuum"" @09:59:12.53 Toroid.in :hold @09:59:12.63 Toroid.seek :bypass @09:59:14.31 Toroid.in :default @09:59:14.45 Toroid.seek :clear @09:59:16.12 Waiting for bill to answer "recharge if needed" @10:00:28.68 Answered recharge if needed"" @10:00:28.70 Vacuuming cartridge 2 @10:00:28.95 Gate.power :heat,:OFF @10:00:28.98 Gate.power :cartridge,:OFF @10:00:29.19 Carousel.in :default @10:00:29.35 Carousel.home.seek 1 @10:00:29.82 Air.in :unlocking @10:00:29.97 Air.seek :clear @10:00:31.01 Lysis1.in :unlocking @10:00:31.17 Lysis1.seek :clear @10:00:31.32 Plunge.in :unlocking @10:00:31.43 Plunge.seek :clear @10:00:32.59 Carousel.seek 2 @10:00:33.18 Lysis1.in :fast @10:00:33.28 Lysis1.seek :locked @10:00:35.96 Plunge.in :default @10:00:36.10 Plunge.seek :locked @10:00:36.25 Air.in :fast @10:00:36.38 Air.seek :locked @10:00:37.40 Carousel.reconfigure :locked @10:00:37.61 Powering cartridge bus @10:00:40.28 Gate.setIR 1750,5000,0,6 @10:00:40.41 Gate.setIR 0,5000,0,6 @10:00:40.52 PuckHeater.configure :default #ambient 22.0C @10:00:40.62 Gate.power :heat,:ON,limit to 5000mA @10:00:41.35 Mounted lysis Cartridge board#Q05058 @10:00:41.43 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:00:41.59 Toroid.in :hold @10:00:41.69 Toroid.seek :cartridge @10:00:43.56 Toroid.in :default @10:00:43.65 Toroid.seek :clear @10:00:45.51 Waiting for bill to answer "record Vacuum" @10:00:58.43 Answered record Vacuum"" @10:00:59.21 Toroid.in :hold @10:00:59.34 Toroid.seek :bypass @10:01:01.01 Toroid.in :default @10:01:01.12 Toroid.seek :clear @10:01:02.77 Waiting for bill to answer "recharge if needed" @10:01:33.66 Answered recharge if needed"" @10:01:33.68 Vacuuming cartridge 3 @10:01:33.92 Gate.power :heat,:OFF @10:01:33.95 Gate.power :cartridge,:OFF @10:01:34.09 Carousel.in :default @10:01:34.26 Carousel.home.seek 2 @10:01:34.74 Air.in :unlocking @10:01:34.91 Air.seek :clear @10:01:35.95 Lysis1.in :unlocking @10:01:36.09 Lysis1.seek :clear @10:01:36.26 Plunge.in :unlocking @10:01:36.42 Plunge.seek :clear @10:01:37.55 Carousel.seek 3 @10:01:38.15 Lysis1.in :fast @10:01:38.25 Lysis1.seek :locked @10:01:40.92 Plunge.in :default @10:01:41.03 Plunge.seek :locked @10:01:41.22 Air.in :fast @10:01:41.32 Air.seek :locked @10:01:42.34 Carousel.reconfigure :locked @10:01:42.58 Powering cartridge bus @10:01:45.28 Gate.setIR 1750,5000,0,6 @10:01:45.41 Gate.setIR 0,5000,0,6 @10:01:45.53 Gate.power :cartridge,:OFF @10:01:46.12 Mounted archive Cartridge @10:01:46.31 Toroid.in :hold @10:01:46.45 Toroid.seek :cartridge @10:01:48.34 Toroid.in :default @10:01:48.48 Toroid.seek :clear @10:01:50.35 Waiting for bill to answer "record Vacuum" @10:01:57.73 Answered record Vacuum"" @10:01:57.87 Toroid.in :hold @10:01:57.97 Toroid.seek :bypass @10:01:59.65 Toroid.in :default @10:01:59.75 Toroid.seek :clear @10:02:01.42 Waiting for bill to answer "recharge if needed" @10:02:01.48 Answered recharge if needed"" @10:02:01.51 Vacuuming cartridge 4 @10:02:01.81 Carousel.in :default @10:02:01.97 Carousel.home.seek 3 @10:02:03.13 Air.in :unlocking @10:02:03.29 Air.seek :clear @10:02:04.33 Lysis1.in :unlocking @10:02:04.53 Lysis1.seek :clear @10:02:04.68 Plunge.in :unlocking @10:02:04.81 Plunge.seek :clear @10:02:05.95 Carousel.seek 4 @10:02:06.57 Lysis1.in :fast @10:02:06.67 Lysis1.seek :locked @10:02:09.33 Plunge.in :default @10:02:09.48 Plunge.seek :locked @10:02:09.63 Air.in :fast @10:02:09.74 Air.seek :locked @10:02:10.75 Carousel.reconfigure :locked @10:02:10.96 Powering cartridge bus @10:02:13.63 Gate.setIR 1750,5000,0,6 @10:02:13.76 Gate.setIR 0,5000,0,6 @10:02:13.87 PuckHeater.configure :default #ambient 22.0C @10:02:13.98 Gate.power :heat,:ON,limit to 5000mA @10:02:14.72 Mounted lysis Cartridge board#Q05092 @10:02:14.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:02:14.96 Toroid.in :hold @10:02:15.08 Toroid.seek :cartridge @10:02:16.95 Toroid.in :default @10:02:17.06 Toroid.seek :clear @10:02:18.91 Waiting for bill to answer "record Vacuum" @10:02:28.34 Answered record Vacuum"" @10:02:28.52 Toroid.in :hold @10:02:28.62 Toroid.seek :bypass @10:02:30.30 Toroid.in :default @10:02:30.50 Toroid.seek :clear @10:02:32.16 Waiting for bill to answer "recharge if needed" @10:02:42.57 Answered recharge if needed"" @10:02:42.58 Vacuuming cartridge 5 @10:02:42.81 Gate.power :heat,:OFF @10:02:42.84 Gate.power :cartridge,:OFF @10:02:42.96 Carousel.in :default @10:02:43.11 Carousel.home.seek 4 @10:02:43.59 Air.in :unlocking @10:02:43.77 Air.seek :clear @10:02:44.82 Lysis1.in :unlocking @10:02:44.98 Lysis1.seek :clear @10:02:45.14 Plunge.in :unlocking @10:02:45.24 Plunge.seek :clear @10:02:47.04 Carousel.seek 5 @10:02:47.65 Lysis1.in :fast @10:02:47.75 Lysis1.seek :locked @10:02:50.47 Plunge.in :default @10:02:50.58 Plunge.seek :locked @10:02:50.78 Air.in :fast @10:02:50.86 Air.seek :locked @10:02:51.87 Carousel.reconfigure :locked @10:02:52.07 Powering cartridge bus @10:02:54.77 Gate.setIR 1750,5000,0,6 @10:02:54.90 Gate.setIR 0,5000,0,6 @10:02:55.02 Gate.power :cartridge,:OFF @10:02:55.61 Mounted archive Cartridge @10:02:55.80 Toroid.in :hold @10:02:55.90 Toroid.seek :cartridge @10:02:57.78 Toroid.in :default @10:02:57.89 Toroid.seek :clear @10:02:59.75 Waiting for bill to answer "record Vacuum" @10:03:07.35 Answered record Vacuum"" @10:03:07.51 Toroid.in :hold @10:03:07.60 Toroid.seek :bypass @10:03:09.29 Toroid.in :default @10:03:09.39 Toroid.seek :clear @10:03:11.06 Waiting for bill to answer "recharge if needed" @10:03:19.75 Answered recharge if needed"" @10:03:19.76 Vacuuming cartridge 6 @10:03:20.06 Carousel.in :default @10:03:20.22 Carousel.home.seek 5 @10:03:20.69 Air.in :unlocking @10:03:20.87 Air.seek :clear @10:03:21.91 Lysis1.in :unlocking @10:03:22.09 Lysis1.seek :clear @10:03:22.16 Plunge.in :unlocking @10:03:22.37 Plunge.seek :clear @10:03:23.59 Carousel.seek 6 @10:03:24.18 Lysis1.in :fast @10:03:24.29 Lysis1.seek :locked @10:03:26.97 Plunge.in :default @10:03:27.12 Plunge.seek :locked @10:03:27.28 Air.in :fast @10:03:27.37 Air.seek :locked @10:03:28.39 Carousel.reconfigure :locked @10:03:28.60 Powering cartridge bus @10:03:31.28 Gate.setIR 1750,5000,0,6 @10:03:31.41 Gate.setIR 0,5000,0,6 @10:03:31.52 PuckHeater.configure :default #ambient 22.0C @10:03:31.64 Gate.power :heat,:ON,limit to 5000mA @10:03:32.40 Mounted lysis Cartridge board#Q05031 @10:03:32.48 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:03:32.66 Toroid.in :hold @10:03:32.77 Toroid.seek :cartridge @10:03:34.66 Toroid.in :default @10:03:34.75 Toroid.seek :clear @10:03:36.61 Waiting for bill to answer "record Vacuum" @10:03:46.77 Answered record Vacuum"" @10:03:46.93 Toroid.in :hold @10:03:47.03 Toroid.seek :bypass @10:03:48.71 Toroid.in :default @10:03:48.81 Toroid.seek :clear @10:03:50.47 Waiting for bill to answer "recharge if needed" @10:04:02.65 Answered recharge if needed"" @10:04:02.66 Vacuuming cartridge 7 @10:04:02.90 Gate.power :heat,:OFF @10:04:02.93 Gate.power :cartridge,:OFF @10:04:03.07 Carousel.in :default @10:04:03.22 Carousel.home.seek 6 @10:04:03.69 Air.in :unlocking @10:04:03.88 Air.seek :clear @10:04:04.95 Lysis1.in :unlocking @10:04:05.14 Lysis1.seek :clear @10:04:05.23 Plunge.in :unlocking @10:04:05.38 Plunge.seek :clear @10:04:06.63 Carousel.seek 7 @10:04:07.22 Lysis1.in :fast @10:04:07.33 Lysis1.seek :locked @10:04:09.99 Plunge.in :default @10:04:10.12 Plunge.seek :locked @10:04:10.31 Air.in :fast @10:04:10.44 Air.seek :locked @10:04:11.46 Carousel.reconfigure :locked @10:04:11.66 Powering cartridge bus @10:04:14.35 Gate.setIR 1750,5000,0,6 @10:04:14.50 Gate.setIR 0,5000,0,6 @10:04:14.62 Gate.power :cartridge,:OFF @10:04:15.21 Mounted archive Cartridge @10:04:15.39 Toroid.in :hold @10:04:15.49 Toroid.seek :cartridge @10:04:18.01 Toroid.in :default @10:04:18.14 Toroid.seek :clear @10:04:19.99 Waiting for bill to answer "record Vacuum" @10:04:27.12 Answered record Vacuum"" @10:04:27.26 Toroid.in :hold @10:04:27.36 Toroid.seek :bypass @10:04:29.03 Toroid.in :default @10:04:29.13 Toroid.seek :clear @10:04:30.86 Waiting for bill to answer "recharge if needed" @10:04:35.42 Answered recharge if needed"" @10:04:35.43 Vacuuming cartridge 8 @10:04:35.73 Carousel.in :default @10:04:35.90 Carousel.home.seek 7 @10:04:36.40 Air.in :unlocking @10:04:36.56 Air.seek :clear @10:04:37.61 Lysis1.in :unlocking @10:04:37.81 Lysis1.seek :clear @10:04:37.87 Plunge.in :unlocking @10:04:38.04 Plunge.seek :clear @10:04:39.27 Carousel.seek 8 @10:04:39.85 Lysis1.in :fast @10:04:39.96 Lysis1.seek :locked @10:04:42.63 Plunge.in :default @10:04:42.72 Plunge.seek :locked @10:04:42.91 Air.in :fast @10:04:43.02 Air.seek :locked @10:04:44.03 Carousel.reconfigure :locked @10:04:44.23 Powering cartridge bus @10:04:46.90 Gate.setIR 1750,5000,0,6 @10:04:47.03 Gate.setIR 0,5000,0,6 @10:04:47.14 PuckHeater.configure :default #ambient 22.0C @10:04:47.24 Gate.power :heat,:ON,limit to 5000mA @10:04:47.97 Mounted lysis Cartridge board#Q05061 @10:04:48.05 Using this cartridge's heat loss curve for HeatingAccelInCarousel @10:04:48.21 Toroid.in :hold @10:04:48.32 Toroid.seek :cartridge @10:04:50.23 Toroid.in :default @10:04:50.33 Toroid.seek :clear @10:04:52.22 Waiting for bill to answer "record Vacuum" @10:05:00.30 Answered record Vacuum"" @10:05:00.48 Toroid.in :hold @10:05:00.58 Toroid.seek :bypass @10:05:02.26 Toroid.in :default @10:05:02.37 Toroid.seek :clear @10:05:04.73 Waiting for bill to answer "recharge if needed" @10:05:13.00 Answered recharge if needed"" @10:05:13.01 Vacuuming cartridge 9 @10:05:13.24 Gate.power :heat,:OFF @10:05:13.27 Gate.power :cartridge,:OFF @10:05:13.41 Carousel.in :default @10:05:13.56 Carousel.home.seek 8 @10:05:14.03 Air.in :unlocking @10:05:14.18 Air.seek :clear @10:05:15.23 Lysis1.in :unlocking @10:05:15.42 Lysis1.seek :clear @10:05:15.53 Plunge.in :unlocking @10:05:15.67 Plunge.seek :clear @10:05:16.85 Carousel.seek 9 @10:05:17.42 Lysis1.in :fast @10:05:17.52 Lysis1.seek :locked @10:05:20.19 Plunge.in :default @10:05:20.31 Plunge.seek :locked @10:05:20.54 Air.in :fast @10:05:20.64 Air.seek :locked @10:05:21.65 Carousel.reconfigure :locked @10:05:21.84 Powering cartridge bus @10:05:24.51 Gate.setIR 1750,5000,0,6 @10:05:24.64 Gate.setIR 0,5000,0,6 @10:05:24.77 Gate.power :cartridge,:OFF @10:05:25.35 Mounted archive Cartridge @10:05:25.54 Toroid.in :hold @10:05:25.63 Toroid.seek :cartridge @10:05:27.52 Toroid.in :default @10:05:27.62 Toroid.seek :clear @10:05:29.46 Waiting for bill to answer "record Vacuum" @10:05:35.52 Answered record Vacuum"" @10:05:35.68 Toroid.in :hold @10:05:35.78 Toroid.seek :bypass @10:05:37.47 Toroid.in :default @10:05:37.57 Toroid.seek :clear @10:05:39.23 Waiting for bill to answer "recharge if needed" @10:05:44.64 Answered recharge if needed"" @10:05:44.65 Vacuuming cartridge 10 @10:05:44.97 Carousel.in :default @10:05:45.14 Carousel.home.seek 9 @10:05:45.62 Air.in :unlocking @10:05:45.79 Air.seek :clear @10:05:47.52 Lysis1.in :unlocking @10:05:47.71 Lysis1.seek :clear @10:05:47.86 Plunge.in :unlocking @10:05:47.97 Plunge.seek :clear @10:05:49.12 Carousel.seek 10 @10:05:49.72 Lysis1.in :fast @10:05:49.83 Lysis1.seek :locked @10:05:52.52 Plunge.in :default @10:05:52.64 Plunge.seek :locked @10:05:52.82 Air.in :fast @10:05:52.92 Air.seek :locked @10:05:53.93 Carousel.reconfigure :locked @10:05:54.13 Powering cartridge bus @10:05:56.80 Gate.setIR 1750,5000,0,6 @10:05:56.93 Gate.setIR 0,5000,0,6 @10:05:57.04 PuckHeater.configure :default #ambient 22.0C @10:05:57.15 Gate.power :heat,:ON,limit to 5000mA @10:05:57.86 Mounted lysis Cartridge board#Q05080 @10:05:57.94 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:05:58.10 Toroid.in :hold @10:05:58.20 Toroid.seek :cartridge @10:06:00.08 Toroid.in :default @10:06:00.18 Toroid.seek :clear @10:06:02.05 Waiting for bill to answer "record Vacuum" @10:06:10.96 Answered record Vacuum"" @10:06:11.11 Toroid.in :hold @10:06:11.21 Toroid.seek :bypass @10:06:12.89 Toroid.in :default @10:06:12.99 Toroid.seek :clear @10:06:14.65 Waiting for bill to answer "recharge if needed" @10:06:25.80 Answered recharge if needed"" @10:06:25.82 Vacuuming cartridge 11 @10:06:26.06 Gate.power :heat,:OFF @10:06:26.08 Gate.power :cartridge,:OFF @10:06:26.24 Carousel.in :default @10:06:26.44 Carousel.home.seek 10 @10:06:26.91 Air.in :unlocking @10:06:27.08 Air.seek :clear @10:06:28.12 Lysis1.in :unlocking @10:06:28.29 Lysis1.seek :clear @10:06:28.48 Plunge.in :unlocking @10:06:28.58 Plunge.seek :clear @10:06:29.73 Carousel.seek 11 @10:06:30.33 Lysis1.in :fast @10:06:31.17 Lysis1.seek :locked @10:06:33.85 Plunge.in :default @10:06:34.01 Plunge.seek :locked @10:06:34.16 Air.in :fast @10:06:34.27 Air.seek :locked @10:06:35.29 Carousel.reconfigure :locked @10:06:35.50 Powering cartridge bus @10:06:38.20 Gate.setIR 1750,5000,0,6 @10:06:38.33 Gate.setIR 0,5000,0,6 @10:06:38.48 Gate.power :cartridge,:OFF @10:06:39.06 Mounted archive Cartridge @10:06:39.23 Toroid.in :hold @10:06:39.34 Toroid.seek :cartridge @10:06:41.22 Toroid.in :default @10:06:41.33 Toroid.seek :clear @10:06:43.16 Waiting for bill to answer "record Vacuum" @10:06:48.65 Answered record Vacuum"" @10:06:48.80 Toroid.in :hold @10:06:48.89 Toroid.seek :bypass @10:06:50.58 Toroid.in :default @10:06:50.68 Toroid.seek :clear @10:06:52.38 Waiting for bill to answer "recharge if needed" @10:06:53.91 Answered recharge if needed"" @10:06:53.93 Vacuuming cartridge 12 @10:06:54.27 Carousel.in :default @10:06:54.46 Carousel.home.seek 11 @10:06:54.94 Air.in :unlocking @10:06:55.07 Air.seek :clear @10:06:56.17 Lysis1.in :unlocking @10:06:56.38 Lysis1.seek :clear @10:06:56.54 Plunge.in :unlocking @10:06:56.64 Plunge.seek :clear @10:06:57.82 Carousel.seek 12 @10:06:58.44 Lysis1.in :fast @10:06:58.54 Lysis1.seek :locked @10:07:01.21 Plunge.in :default @10:07:01.32 Plunge.seek :locked @10:07:01.52 Air.in :fast @10:07:01.63 Air.seek :locked @10:07:02.64 Carousel.reconfigure :locked @10:07:02.84 Powering cartridge bus @10:07:05.51 Gate.setIR 1750,5000,0,6 @10:07:05.64 Gate.setIR 0,5000,0,6 @10:07:06.49 PuckHeater.configure :default #ambient 22.0C @10:07:06.60 Gate.power :heat,:ON,limit to 5000mA @10:07:07.31 Mounted lysis Cartridge board#Q05038 @10:07:07.39 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:07:07.54 Toroid.in :hold @10:07:07.66 Toroid.seek :cartridge @10:07:09.54 Toroid.in :default @10:07:09.64 Toroid.seek :clear @10:07:11.50 Waiting for bill to answer "record Vacuum" @10:07:21.00 Answered record Vacuum"" @10:07:21.15 Toroid.in :hold @10:07:21.24 Toroid.seek :bypass @10:07:22.94 Toroid.in :default @10:07:23.03 Toroid.seek :clear @10:07:24.70 Waiting for bill to answer "recharge if needed" @10:07:32.26 Answered recharge if needed"" @10:07:32.28 Vacuuming cartridge 13 @10:07:32.56 Gate.power :heat,:OFF @10:07:32.61 Gate.power :cartridge,:OFF @10:07:32.74 Carousel.in :default @10:07:32.90 Carousel.home.seek 12 @10:07:33.36 Air.in :unlocking @10:07:33.55 Air.seek :clear @10:07:34.60 Lysis1.in :unlocking @10:07:34.80 Lysis1.seek :clear @10:07:34.87 Plunge.in :unlocking @10:07:35.04 Plunge.seek :clear @10:07:36.28 Carousel.seek 13 @10:07:36.87 Lysis1.in :fast @10:07:36.98 Lysis1.seek :locked @10:07:39.66 Plunge.in :default @10:07:39.81 Plunge.seek :locked @10:07:39.97 Air.in :fast @10:07:40.06 Air.seek :locked @10:07:41.07 Carousel.reconfigure :locked @10:07:41.29 Powering cartridge bus @10:07:43.96 Gate.setIR 1750,5000,0,6 @10:07:44.09 Gate.setIR 0,5000,0,6 @10:07:44.21 Gate.power :cartridge,:OFF @10:07:44.81 Mounted archive Cartridge @10:07:45.00 Toroid.in :hold @10:07:45.10 Toroid.seek :cartridge @10:07:46.98 Toroid.in :default @10:07:47.08 Toroid.seek :clear @10:07:48.95 Waiting for bill to answer "record Vacuum" @10:07:54.48 Answered record Vacuum"" @10:07:54.63 Toroid.in :hold @10:07:55.40 Toroid.seek :bypass @10:07:57.08 Toroid.in :default @10:07:57.18 Toroid.seek :clear @10:07:58.83 Waiting for bill to answer "recharge if needed" @10:08:00.44 Answered recharge if needed"" @10:08:00.46 Vacuuming cartridge 14 @10:08:00.79 Carousel.in :default @10:08:00.96 Carousel.home.seek 13 @10:08:01.45 Air.in :unlocking @10:08:01.63 Air.seek :clear @10:08:02.70 Lysis1.in :unlocking @10:08:02.86 Lysis1.seek :clear @10:08:03.01 Plunge.in :unlocking @10:08:03.13 Plunge.seek :clear @10:08:04.27 Carousel.seek 14 @10:08:04.88 Lysis1.in :fast @10:08:04.98 Lysis1.seek :locked @10:08:07.65 Plunge.in :default @10:08:07.80 Plunge.seek :locked @10:08:07.94 Air.in :fast @10:08:08.04 Air.seek :locked @10:08:09.06 Carousel.reconfigure :locked @10:08:09.27 Powering cartridge bus @10:08:11.96 Gate.setIR 1750,5000,0,6 @10:08:12.09 Gate.setIR 0,5000,0,6 @10:08:12.20 PuckHeater.configure :default #ambient 22.0C @10:08:12.32 Gate.power :heat,:ON,limit to 5000mA @10:08:13.04 Mounted lysis Cartridge board#Q05037 @10:08:13.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:08:13.28 Toroid.in :hold @10:08:13.38 Toroid.seek :cartridge @10:08:15.25 Toroid.in :default @10:08:15.36 Toroid.seek :clear @10:08:17.22 Waiting for bill to answer "record Vacuum" @10:08:23.66 Answered record Vacuum"" @10:08:23.81 Toroid.in :hold @10:08:23.91 Toroid.seek :bypass @10:08:25.59 Toroid.in :default @10:08:25.70 Toroid.seek :clear @10:08:27.36 Waiting for bill to answer "recharge if needed" @10:08:44.57 Answered recharge if needed"" @10:08:44.59 Vacuuming cartridge 15 @10:08:44.83 Gate.power :heat,:OFF @10:08:44.86 Gate.power :cartridge,:OFF @10:08:45.00 Carousel.in :default @10:08:45.15 Carousel.home.seek 14 @10:08:46.28 Air.in :unlocking @10:08:46.51 Air.seek :clear @10:08:47.56 Lysis1.in :unlocking @10:08:47.71 Lysis1.seek :clear @10:08:47.86 Plunge.in :unlocking @10:08:47.97 Plunge.seek :clear @10:08:49.12 Carousel.seek 15 @10:08:49.72 Lysis1.in :fast @10:08:49.82 Lysis1.seek :locked @10:08:52.51 Plunge.in :default @10:08:52.61 Plunge.seek :locked @10:08:52.84 Air.in :fast @10:08:52.94 Air.seek :locked @10:08:53.95 Carousel.reconfigure :locked @10:08:54.15 Powering cartridge bus @10:08:56.82 Gate.setIR 1750,5000,0,6 @10:08:56.96 Gate.setIR 0,5000,0,6 @10:08:57.08 Gate.power :cartridge,:OFF @10:08:57.68 Mounted archive Cartridge @10:08:57.86 Toroid.in :hold @10:08:57.97 Toroid.seek :cartridge @10:08:59.85 Toroid.in :default @10:08:59.96 Toroid.seek :clear @10:09:01.81 Waiting for bill to answer "record Vacuum" @10:09:07.49 Answered record Vacuum"" @10:09:07.64 Toroid.in :hold @10:09:07.73 Toroid.seek :bypass @10:09:09.42 Toroid.in :default @10:09:09.53 Toroid.seek :clear @10:09:11.19 Waiting for bill to answer "recharge if needed" @10:09:14.66 Answered recharge if needed"" @10:09:14.69 Vacuuming cartridge 16 @10:09:14.98 Carousel.in :default @10:09:15.15 Carousel.home.seek 15 @10:09:15.61 Air.in :unlocking @10:09:15.79 Air.seek :clear @10:09:16.84 Lysis1.in :unlocking @10:09:17.01 Lysis1.seek :clear @10:09:17.17 Plunge.in :unlocking @10:09:17.28 Plunge.seek :clear @10:09:19.10 Carousel.seek 16 @10:09:19.74 Lysis1.in :fast @10:09:19.84 Lysis1.seek :locked @10:09:22.51 Plunge.in :default @10:09:22.65 Plunge.seek :locked @10:09:22.81 Air.in :fast @10:09:22.92 Air.seek :locked @10:09:23.92 Carousel.reconfigure :locked @10:09:24.14 Powering cartridge bus @10:09:26.81 Gate.setIR 1750,5000,0,6 @10:09:26.95 Gate.setIR 0,5000,0,6 @10:09:27.06 PuckHeater.configure :default #ambient 22.0C @10:09:27.18 Gate.power :heat,:ON,limit to 5000mA @10:09:27.89 Mounted lysis Cartridge board#Q05054 @10:09:27.97 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:09:28.12 Toroid.in :hold @10:09:28.22 Toroid.seek :cartridge @10:09:30.10 Toroid.in :default @10:09:30.21 Toroid.seek :clear @10:09:32.07 Waiting for bill to answer "record Vacuum" @10:09:42.51 Answered record Vacuum"" @10:09:42.66 Toroid.in :hold @10:09:42.75 Toroid.seek :bypass @10:09:44.44 Toroid.in :default @10:09:44.54 Toroid.seek :clear @10:09:46.22 Waiting for bill to answer "recharge if needed" @10:09:57.87 Answered recharge if needed"" @10:09:57.89 Vacuuming cartridge 17 @10:09:58.12 Gate.power :heat,:OFF @10:09:58.15 Gate.power :cartridge,:OFF @10:09:58.30 Carousel.in :default @10:09:58.48 Carousel.home.seek 16 @10:09:58.95 Air.in :unlocking @10:09:59.11 Air.seek :clear @10:10:00.15 Lysis1.in :unlocking @10:10:00.30 Lysis1.seek :clear @10:10:00.50 Plunge.in :unlocking @10:10:00.62 Plunge.seek :clear @10:10:01.77 Carousel.seek 17 @10:10:02.37 Lysis1.in :fast @10:10:02.49 Lysis1.seek :locked @10:10:05.15 Plunge.in :default @10:10:05.25 Plunge.seek :locked @10:10:06.11 Air.in :fast @10:10:06.25 Air.seek :locked @10:10:07.26 Carousel.reconfigure :locked @10:10:07.45 Powering cartridge bus @10:10:10.13 Gate.setIR 1750,5000,0,6 @10:10:10.27 Gate.setIR 0,5000,0,6 @10:10:10.42 Gate.power :cartridge,:OFF @10:10:11.00 Mounted archive Cartridge @10:10:11.18 Toroid.in :hold @10:10:11.27 Toroid.seek :cartridge @10:10:13.15 Toroid.in :default @10:10:13.26 Toroid.seek :clear @10:10:15.09 Waiting for bill to answer "record Vacuum" @10:10:18.15 Answered record Vacuum"" @10:10:18.30 Toroid.in :hold @10:10:18.43 Toroid.seek :bypass @10:10:20.11 Toroid.in :default @10:10:20.20 Toroid.seek :clear @10:10:21.86 Waiting for bill to answer "recharge if needed" @10:10:24.40 Answered recharge if needed"" @10:10:24.42 Vacuuming cartridge 18 @10:10:24.71 Carousel.in :default @10:10:24.89 Carousel.home.seek 17 @10:10:25.36 Air.in :unlocking @10:10:25.52 Air.seek :clear @10:10:26.55 Lysis1.in :unlocking @10:10:26.72 Lysis1.seek :clear @10:10:26.87 Plunge.in :unlocking @10:10:26.98 Plunge.seek :clear @10:10:28.13 Carousel.seek 18 @10:10:28.72 Lysis1.in :fast @10:10:28.82 Lysis1.seek :locked @10:10:31.49 Plunge.in :default @10:10:31.67 Plunge.seek :locked @10:10:31.82 Air.in :fast @10:10:31.92 Air.seek :locked @10:10:32.94 Carousel.reconfigure :locked @10:10:33.15 Powering cartridge bus @10:10:35.82 Gate.setIR 1750,5000,0,6 @10:10:35.95 Gate.setIR 0,5000,0,6 @10:10:36.07 PuckHeater.configure :default #ambient 22.0C @10:10:36.18 Gate.power :heat,:ON,limit to 5000mA @10:10:36.91 Mounted lysis Cartridge board#Q05063 @10:10:37.00 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:10:37.83 Toroid.in :hold @10:10:37.97 Toroid.seek :cartridge @10:10:39.85 Toroid.in :default @10:10:39.96 Toroid.seek :clear @10:10:41.81 Waiting for bill to answer "record Vacuum" @10:10:53.58 Answered record Vacuum"" @10:10:53.74 Toroid.in :hold @10:10:53.84 Toroid.seek :bypass @10:10:55.52 Toroid.in :default @10:10:55.62 Toroid.seek :clear @10:10:57.29 Waiting for bill to answer "recharge if needed" @10:11:03.51 Answered recharge if needed"" @10:11:03.52 Vacuuming cartridge 19 @10:11:03.75 Gate.power :heat,:OFF @10:11:03.78 Gate.power :cartridge,:OFF @10:11:03.92 Carousel.in :default @10:11:04.07 Carousel.home.seek 18 @10:11:04.60 Air.in :unlocking @10:11:04.81 Air.seek :clear @10:11:05.86 Lysis1.in :unlocking @10:11:06.01 Lysis1.seek :clear @10:11:06.17 Plunge.in :unlocking @10:11:06.28 Plunge.seek :clear @10:11:07.45 Carousel.seek 19 @10:11:08.04 Lysis1.in :fast @10:11:08.14 Lysis1.seek :locked @10:11:10.80 Plunge.in :default @10:11:10.89 Plunge.seek :locked @10:11:11.09 Air.in :fast @10:11:11.20 Air.seek :locked @10:11:12.22 Carousel.reconfigure :locked @10:11:12.45 Powering cartridge bus @10:11:15.12 Gate.setIR 1750,5000,0,6 @10:11:15.25 Gate.setIR 0,5000,0,6 @10:11:15.37 Gate.power :cartridge,:OFF @10:11:15.96 Mounted archive Cartridge @10:11:16.14 Toroid.in :hold @10:11:16.24 Toroid.seek :cartridge @10:11:18.12 Toroid.in :default @10:11:18.23 Toroid.seek :clear @10:11:20.09 Waiting for bill to answer "record Vacuum" @10:11:26.04 Answered record Vacuum"" @10:11:26.18 Toroid.in :hold @10:11:26.28 Toroid.seek :bypass @10:11:27.98 Toroid.in :default @10:11:28.09 Toroid.seek :clear @10:11:29.75 Waiting for bill to answer "recharge if needed" @10:11:32.18 Answered recharge if needed"" @10:11:32.19 Vacuuming cartridge 20 @10:11:33.19 Carousel.in :default @10:11:33.40 Carousel.home.seek 19 @10:11:33.89 Air.in :unlocking @10:11:34.07 Air.seek :clear @10:11:35.13 Lysis1.in :unlocking @10:11:35.33 Lysis1.seek :clear @10:11:35.43 Plunge.in :unlocking @10:11:35.58 Plunge.seek :clear @10:11:36.76 Carousel.seek 20 @10:11:37.34 Lysis1.in :fast @10:11:37.44 Lysis1.seek :locked @10:11:40.12 Plunge.in :default @10:11:40.23 Plunge.seek :locked @10:11:40.47 Air.in :fast @10:11:40.56 Air.seek :locked @10:11:41.55 Carousel.reconfigure :locked @10:11:41.75 Powering cartridge bus @10:11:44.43 Gate.setIR 1750,5000,0,6 @10:11:44.57 Gate.setIR 0,5000,0,6 @10:11:44.67 PuckHeater.configure :default #ambient 22.0C @10:11:44.78 Gate.power :heat,:ON,limit to 5000mA @10:11:45.49 Mounted lysis Cartridge board#Q05042 @10:11:45.57 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:11:45.74 Toroid.in :hold @10:11:45.83 Toroid.seek :cartridge @10:11:47.70 Toroid.in :default @10:11:47.81 Toroid.seek :clear @10:11:49.66 Waiting for bill to answer "record Vacuum" @10:11:56.37 Answered record Vacuum"" @10:11:56.55 Toroid.in :hold @10:11:56.64 Toroid.seek :bypass @10:11:58.32 Toroid.in :default @10:11:58.46 Toroid.seek :clear @10:12:00.12 Waiting for bill to answer "recharge if needed" @10:12:07.31 Answered recharge if needed"" @10:12:07.32 Vacuuming cartridge 21 @10:12:07.55 Gate.power :heat,:OFF @10:12:07.58 Gate.power :cartridge,:OFF @10:12:07.73 Carousel.in :default @10:12:07.88 Carousel.home.seek 20 @10:12:08.38 Air.in :unlocking @10:12:08.55 Air.seek :clear @10:12:10.36 Lysis1.in :unlocking @10:12:10.55 Lysis1.seek :clear @10:12:10.68 Plunge.in :unlocking @10:12:10.79 Plunge.seek :clear @10:12:11.97 Carousel.seek 21 @10:12:12.59 Lysis1.in :fast @10:12:12.69 Lysis1.seek :locked @10:12:15.36 Plunge.in :default @10:12:15.51 Plunge.seek :locked @10:12:15.67 Air.in :fast @10:12:15.77 Air.seek :locked @10:12:16.79 Carousel.reconfigure :locked @10:12:16.99 Powering cartridge bus @10:12:19.66 Gate.setIR 1750,5000,0,6 @10:12:19.80 Gate.setIR 0,5000,0,6 @10:12:19.92 Gate.power :cartridge,:OFF @10:12:20.51 Mounted archive Cartridge @10:12:20.69 Toroid.in :hold @10:12:20.79 Toroid.seek :cartridge @10:12:22.66 Toroid.in :default @10:12:22.77 Toroid.seek :clear @10:12:24.62 Waiting for bill to answer "record Vacuum" @10:12:31.47 Answered record Vacuum"" @10:12:31.62 Toroid.in :hold @10:12:31.72 Toroid.seek :bypass @10:12:33.40 Toroid.in :default @10:12:33.51 Toroid.seek :clear @10:12:35.18 Waiting for bill to answer "recharge if needed" @10:12:38.64 Answered recharge if needed"" @10:12:38.67 Vacuuming cartridge 22 @10:12:38.96 Carousel.in :default @10:12:39.13 Carousel.home.seek 21 @10:12:39.60 Air.in :unlocking @10:12:39.77 Air.seek :clear @10:12:40.81 Lysis1.in :unlocking @10:12:40.98 Lysis1.seek :clear @10:12:41.12 Plunge.in :unlocking @10:12:41.23 Plunge.seek :clear @10:12:42.41 Carousel.seek 22 @10:12:43.00 Lysis1.in :fast @10:12:43.11 Lysis1.seek :locked @10:12:45.79 Plunge.in :default @10:12:45.95 Plunge.seek :locked @10:12:46.11 Air.in :fast @10:12:46.20 Air.seek :locked @10:12:47.20 Carousel.reconfigure :locked @10:12:47.40 Powering cartridge bus @10:12:50.07 Gate.setIR 1750,5000,0,6 @10:12:50.21 Gate.setIR 0,5000,0,6 @10:12:50.31 PuckHeater.configure :default #ambient 22.0C @10:12:50.45 Gate.power :heat,:ON,limit to 5000mA @10:12:51.16 Mounted lysis Cartridge board#Q05090 @10:12:51.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:12:51.40 Toroid.in :hold @10:12:51.49 Toroid.seek :cartridge @10:12:53.36 Toroid.in :default @10:12:53.47 Toroid.seek :clear @10:12:55.32 Waiting for bill to answer "record Vacuum" @10:13:02.50 Answered record Vacuum"" @10:13:02.66 Toroid.in :hold @10:13:02.75 Toroid.seek :bypass @10:13:04.44 Toroid.in :default @10:13:04.54 Toroid.seek :clear @10:13:06.20 Waiting for bill to answer "recharge if needed" @10:13:13.05 Answered recharge if needed"" @10:13:13.06 Vacuuming cartridge 23 @10:13:13.29 Gate.power :heat,:OFF @10:13:13.32 Gate.power :cartridge,:OFF @10:13:13.46 Carousel.in :default @10:13:13.61 Carousel.home.seek 22 @10:13:14.07 Air.in :unlocking @10:13:14.22 Air.seek :clear @10:13:15.28 Lysis1.in :unlocking @10:13:15.49 Lysis1.seek :clear @10:13:15.54 Plunge.in :unlocking @10:13:15.72 Plunge.seek :clear @10:13:16.97 Carousel.seek 23 @10:13:17.56 Lysis1.in :fast @10:13:17.67 Lysis1.seek :locked @10:13:20.33 Plunge.in :default @10:13:20.48 Plunge.seek :locked @10:13:20.68 Air.in :fast @10:13:20.76 Air.seek :locked @10:13:21.77 Carousel.reconfigure :locked @10:13:21.97 Powering cartridge bus @10:13:24.75 Gate.setIR 1750,5000,0,6 @10:13:24.91 Gate.setIR 0,5000,0,6 @10:13:25.03 Gate.power :cartridge,:OFF @10:13:25.62 Mounted archive Cartridge @10:13:25.81 Toroid.in :hold @10:13:25.92 Toroid.seek :cartridge @10:13:27.80 Toroid.in :default @10:13:27.90 Toroid.seek :clear @10:13:29.76 Waiting for bill to answer "record Vacuum" @10:13:43.67 Answered record Vacuum"" @10:13:43.82 Toroid.in :hold @10:13:43.91 Toroid.seek :bypass @10:13:45.60 Toroid.in :default @10:13:45.69 Toroid.seek :clear @10:13:47.36 Waiting for bill to answer "recharge if needed" @10:13:50.63 Answered recharge if needed"" @10:13:50.65 Vacuuming cartridge 24 @10:13:50.95 Carousel.in :default @10:13:51.12 Carousel.home.seek 23 @10:13:51.58 Air.in :unlocking @10:13:51.73 Air.seek :clear @10:13:52.77 Lysis1.in :unlocking @10:13:52.94 Lysis1.seek :clear @10:13:53.09 Plunge.in :unlocking @10:13:53.20 Plunge.seek :clear @10:13:54.39 Carousel.seek 24 @10:13:54.97 Lysis1.in :fast @10:13:55.08 Lysis1.seek :locked @10:13:57.75 Plunge.in :default @10:13:57.91 Plunge.seek :locked @10:13:58.04 Air.in :fast @10:13:58.15 Air.seek :locked @10:13:59.16 Carousel.reconfigure :locked @10:13:59.36 Powering cartridge bus @10:14:02.03 Gate.setIR 1750,5000,0,6 @10:14:02.16 Gate.setIR 0,5000,0,6 @10:14:02.27 PuckHeater.configure :default #ambient 22.0C @10:14:02.42 Gate.power :heat,:ON,limit to 5000mA @10:14:03.12 Mounted lysis Cartridge board#Q05076 @10:14:03.19 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:14:03.36 Toroid.in :hold @10:14:03.45 Toroid.seek :cartridge @10:14:05.33 Toroid.in :default @10:14:05.44 Toroid.seek :clear @10:14:07.29 Waiting for bill to answer "record Vacuum" @10:14:12.64 Answered record Vacuum"" @10:14:13.57 Toroid.in :hold @10:14:13.72 Toroid.seek :bypass @10:14:15.45 Toroid.in :default @10:14:15.56 Toroid.seek :clear @10:14:17.23 Waiting for bill to answer "recharge if needed" @10:14:28.82 Answered recharge if needed"" @10:14:28.84 Vacuuming cartridge 25 @10:14:29.08 Gate.power :heat,:OFF @10:14:29.11 Gate.power :cartridge,:OFF @10:14:29.25 Carousel.in :default @10:14:29.40 Carousel.home.seek 24 @10:14:29.88 Air.in :unlocking @10:14:30.03 Air.seek :clear @10:14:31.10 Lysis1.in :unlocking @10:14:31.30 Lysis1.seek :clear @10:14:31.36 Plunge.in :unlocking @10:14:31.54 Plunge.seek :clear @10:14:32.78 Carousel.seek 25 @10:14:33.37 Lysis1.in :fast @10:14:33.47 Lysis1.seek :locked @10:14:36.15 Plunge.in :default @10:14:36.29 Plunge.seek :locked @10:14:36.49 Air.in :fast @10:14:36.58 Air.seek :locked @10:14:37.59 Carousel.reconfigure :locked @10:14:37.79 Powering cartridge bus @10:14:40.49 Gate.setIR 1750,5000,0,6 @10:14:40.62 Gate.setIR 0,5000,0,6 @10:14:40.79 Gate.power :cartridge,:OFF @10:14:41.39 Mounted archive Cartridge @10:14:41.60 Toroid.in :hold @10:14:41.70 Toroid.seek :cartridge @10:14:43.60 Toroid.in :default @10:14:43.70 Toroid.seek :clear @10:14:45.55 Waiting for bill to answer "record Vacuum" @10:14:51.04 Answered record Vacuum"" @10:14:51.21 Toroid.in :hold @10:14:51.30 Toroid.seek :bypass @10:14:52.99 Toroid.in :default @10:14:53.10 Toroid.seek :clear @10:14:54.76 Waiting for bill to answer "recharge if needed" @10:15:03.74 Answered recharge if needed"" @10:15:03.76 Vacuuming cartridge 26 @10:15:04.05 Carousel.in :default @10:15:04.23 Carousel.home.seek 25 @10:15:05.36 Air.in :unlocking @10:15:05.53 Air.seek :clear @10:15:06.57 Lysis1.in :unlocking @10:15:06.74 Lysis1.seek :clear @10:15:06.88 Plunge.in :unlocking @10:15:07.00 Plunge.seek :clear @10:15:08.14 Carousel.seek 26 @10:15:08.76 Lysis1.in :fast @10:15:08.87 Lysis1.seek :locked @10:15:11.55 Plunge.in :default @10:15:11.72 Plunge.seek :locked @10:15:11.86 Air.in :fast @10:15:11.96 Air.seek :locked @10:15:12.98 Carousel.reconfigure :locked @10:15:13.19 Powering cartridge bus @10:15:15.86 Gate.setIR 1750,5000,0,6 @10:15:15.99 Gate.setIR 0,5000,0,6 @10:15:16.10 PuckHeater.configure :default #ambient 22.0C @10:15:16.21 Gate.power :heat,:ON,limit to 5000mA @10:15:16.92 Mounted lysis Cartridge board#Q05057 @10:15:17.00 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:15:17.17 Toroid.in :hold @10:15:17.27 Toroid.seek :cartridge @10:15:19.16 Toroid.in :default @10:15:19.26 Toroid.seek :clear @10:15:21.11 Waiting for bill to answer "record Vacuum" @10:15:26.28 Answered record Vacuum"" @10:15:26.47 Toroid.in :hold @10:15:26.56 Toroid.seek :bypass @10:15:28.25 Toroid.in :default @10:15:28.37 Toroid.seek :clear @10:15:30.06 Waiting for bill to answer "recharge if needed" @10:15:37.12 Answered recharge if needed"" @10:15:37.14 Vacuuming cartridge 27 @10:15:37.38 Gate.power :heat,:OFF @10:15:37.41 Gate.power :cartridge,:OFF @10:15:37.54 Carousel.in :default @10:15:37.69 Carousel.home.seek 26 @10:15:38.18 Air.in :unlocking @10:15:38.35 Air.seek :clear @10:15:39.46 Lysis1.in :unlocking @10:15:39.64 Lysis1.seek :clear @10:15:39.70 Plunge.in :unlocking @10:15:39.87 Plunge.seek :clear @10:15:41.74 Carousel.seek 27 @10:15:42.37 Lysis1.in :fast @10:15:42.49 Lysis1.seek :locked @10:15:45.16 Plunge.in :default @10:15:45.29 Plunge.seek :locked @10:15:45.46 Air.in :fast @10:15:45.56 Air.seek :locked @10:15:46.58 Carousel.reconfigure :locked @10:15:46.79 Powering cartridge bus @10:15:49.46 Gate.setIR 1750,5000,0,6 @10:15:49.60 Gate.setIR 0,5000,0,6 @10:15:49.73 Gate.power :cartridge,:OFF @10:15:50.32 Mounted archive Cartridge @10:15:50.56 Toroid.in :hold @10:15:50.66 Toroid.seek :cartridge @10:15:52.56 Toroid.in :default @10:15:52.66 Toroid.seek :clear @10:15:54.50 Waiting for bill to answer "record Vacuum" @10:15:57.51 Answered record Vacuum"" @10:15:57.70 Toroid.in :hold @10:15:57.81 Toroid.seek :bypass @10:15:59.50 Toroid.in :default @10:15:59.61 Toroid.seek :clear @10:16:01.27 Waiting for bill to answer "recharge if needed" @10:16:08.87 Answered recharge if needed"" @10:16:08.89 Vacuuming cartridge 28 @10:16:09.19 Carousel.in :default @10:16:09.35 Carousel.home.seek 27 @10:16:09.81 Air.in :unlocking @10:16:09.97 Air.seek :clear @10:16:11.02 Lysis1.in :unlocking @10:16:11.19 Lysis1.seek :clear @10:16:11.36 Plunge.in :unlocking @10:16:11.47 Plunge.seek :clear @10:16:12.62 Carousel.seek 28 @10:16:13.20 Lysis1.in :fast @10:16:13.30 Lysis1.seek :locked @10:16:15.96 Plunge.in :default @10:16:16.04 Plunge.seek :locked @10:16:16.24 Air.in :fast @10:16:16.34 Air.seek :locked @10:16:17.40 Carousel.reconfigure :locked @10:16:17.61 Powering cartridge bus @10:16:20.29 Gate.setIR 1750,5000,0,6 @10:16:20.43 Gate.setIR 0,5000,0,6 @10:16:20.53 PuckHeater.configure :default #ambient 22.0C @10:16:20.66 Gate.power :heat,:ON,limit to 5000mA @10:16:21.39 Mounted lysis Cartridge board#Q05045 @10:16:21.47 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:16:21.88 Toroid.in :hold @10:16:21.96 Toroid.seek :cartridge @10:16:23.85 Toroid.in :default @10:16:23.95 Toroid.seek :clear @10:16:25.80 Waiting for bill to answer "record Vacuum" @10:16:37.44 Answered record Vacuum"" @10:16:37.59 Toroid.in :hold @10:16:37.69 Toroid.seek :bypass @10:16:39.37 Toroid.in :default @10:16:39.48 Toroid.seek :clear @10:16:41.18 Waiting for bill to answer "recharge if needed" @10:16:47.88 Answered recharge if needed"" @10:16:47.90 Vacuuming cartridge 29 @10:16:48.14 Gate.power :heat,:OFF @10:16:48.16 Gate.power :cartridge,:OFF @10:16:48.32 Carousel.in :default @10:16:48.53 Carousel.home.seek 28 @10:16:49.00 Air.in :unlocking @10:16:49.17 Air.seek :clear @10:16:50.22 Lysis1.in :unlocking @10:16:50.49 Lysis1.seek :clear @10:16:50.72 Plunge.in :unlocking @10:16:50.81 Plunge.seek :clear @10:16:51.96 Carousel.seek 29 @10:16:52.56 Lysis1.in :fast @10:16:52.67 Lysis1.seek :locked @10:16:55.34 Plunge.in :default @10:16:55.51 Plunge.seek :locked @10:16:55.68 Air.in :fast @10:16:55.78 Air.seek :locked @10:16:56.80 Carousel.reconfigure :locked @10:16:56.99 Powering cartridge bus @10:16:59.66 Gate.setIR 1750,5000,0,6 @10:16:59.79 Gate.setIR 0,5000,0,6 @10:16:59.91 Gate.power :cartridge,:OFF @10:17:00.51 Mounted archive Cartridge @10:17:00.70 Toroid.in :hold @10:17:00.80 Toroid.seek :cartridge @10:17:03.35 Toroid.in :default @10:17:03.48 Toroid.seek :clear @10:17:05.33 Waiting for bill to answer "record Vacuum" @10:17:13.90 Answered record Vacuum"" @10:17:14.05 Toroid.in :hold @10:17:14.15 Toroid.seek :bypass @10:17:15.83 Toroid.in :default @10:17:15.93 Toroid.seek :clear @10:17:17.59 Waiting for bill to answer "recharge if needed" @10:17:21.68 Answered recharge if needed"" @10:17:21.70 Vacuuming cartridge 30 @10:17:21.99 Carousel.in :default @10:17:22.16 Carousel.home.seek 29 @10:17:22.66 Air.in :unlocking @10:17:22.86 Air.seek :clear @10:17:23.92 Lysis1.in :unlocking @10:17:24.07 Lysis1.seek :clear @10:17:24.22 Plunge.in :unlocking @10:17:24.33 Plunge.seek :clear @10:17:25.51 Carousel.seek 30 @10:17:26.10 Lysis1.in :fast @10:17:26.19 Lysis1.seek :locked @10:17:28.86 Plunge.in :default @10:17:29.01 Plunge.seek :locked @10:17:29.17 Air.in :fast @10:17:29.26 Air.seek :locked @10:17:30.28 Carousel.reconfigure :locked @10:17:30.52 Powering cartridge bus @10:17:33.19 Gate.setIR 1750,5000,0,6 @10:17:33.33 Gate.setIR 0,5000,0,6 @10:17:33.43 PuckHeater.configure :default #ambient 22.0C @10:17:33.55 Gate.power :heat,:ON,limit to 5000mA @10:17:34.26 Mounted lysis Cartridge board#Q05064 @10:17:34.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:17:34.54 Toroid.in :hold @10:17:34.63 Toroid.seek :cartridge @10:17:36.51 Toroid.in :default @10:17:36.62 Toroid.seek :clear @10:17:38.48 Waiting for bill to answer "record Vacuum" @10:17:49.42 Answered record Vacuum"" @10:17:49.58 Toroid.in :hold @10:17:49.68 Toroid.seek :bypass @10:17:51.37 Toroid.in :default @10:17:51.47 Toroid.seek :clear @10:17:53.79 Waiting for bill to answer "recharge if needed" @10:18:05.19 Answered recharge if needed"" @10:18:05.21 Vacuuming cartridge 31 @10:18:05.44 Gate.power :heat,:OFF @10:18:05.49 Gate.power :cartridge,:OFF @10:18:05.62 Carousel.in :default @10:18:05.78 Carousel.home.seek 30 @10:18:06.26 Air.in :unlocking @10:18:06.45 Air.seek :clear @10:18:07.49 Lysis1.in :unlocking @10:18:07.67 Lysis1.seek :clear @10:18:07.82 Plunge.in :unlocking @10:18:07.92 Plunge.seek :clear @10:18:09.08 Carousel.seek 31 @10:18:09.67 Lysis1.in :fast @10:18:09.77 Lysis1.seek :locked @10:18:12.47 Plunge.in :default @10:18:12.61 Plunge.seek :locked @10:18:12.75 Air.in :fast @10:18:12.84 Air.seek :locked @10:18:13.85 Carousel.reconfigure :locked @10:18:14.05 Powering cartridge bus @10:18:16.73 Gate.setIR 1750,5000,0,6 @10:18:16.87 Gate.setIR 0,5000,0,6 @10:18:16.98 Gate.power :cartridge,:OFF @10:18:17.58 Mounted archive Cartridge @10:18:17.77 Toroid.in :hold @10:18:17.87 Toroid.seek :cartridge @10:18:19.76 Toroid.in :default @10:18:19.87 Toroid.seek :clear @10:18:21.72 Waiting for bill to answer "record Vacuum" @10:18:29.16 Answered record Vacuum"" @10:18:29.30 Toroid.in :hold @10:18:29.40 Toroid.seek :bypass @10:18:31.08 Toroid.in :default @10:18:31.19 Toroid.seek :clear @10:18:32.85 Waiting for bill to answer "recharge if needed" @10:18:35.46 Answered recharge if needed"" @10:18:35.47 Vacuuming cartridge 32 @10:18:35.79 Carousel.in :default @10:18:35.97 Carousel.home.seek 31 @10:18:36.49 Air.in :unlocking @10:18:36.65 Air.seek :clear @10:18:38.41 Lysis1.in :unlocking @10:18:38.59 Lysis1.seek :clear @10:18:38.75 Plunge.in :unlocking @10:18:38.86 Plunge.seek :clear @10:18:40.01 Carousel.seek 32 @10:18:40.60 Lysis1.in :fast @10:18:40.71 Lysis1.seek :locked @10:18:43.37 Plunge.in :default @10:18:43.53 Plunge.seek :locked @10:18:43.69 Air.in :fast @10:18:43.77 Air.seek :locked @10:18:44.79 Carousel.reconfigure :locked @10:18:44.99 Powering cartridge bus @10:18:47.66 Gate.setIR 1750,5000,0,6 @10:18:47.80 Gate.setIR 0,5000,0,6 @10:18:47.90 PuckHeater.configure :default #ambient 22.0C @10:18:48.02 Gate.power :heat,:ON,limit to 5000mA @10:18:48.76 Mounted lysis Cartridge board#Q05034 @10:18:48.85 Using this cartridge's heat loss curve for HeatingAccelInCarousel @10:18:49.02 Toroid.in :hold @10:18:49.13 Toroid.seek :cartridge @10:18:51.01 Toroid.in :default @10:18:51.12 Toroid.seek :clear @10:18:52.97 Waiting for bill to answer "record Vacuum" @10:19:01.52 Answered record Vacuum"" @10:19:01.67 Toroid.in :hold @10:19:01.77 Toroid.seek :bypass @10:19:03.45 Toroid.in :default @10:19:03.55 Toroid.seek :clear @10:19:05.25 Waiting for bill to answer "recharge if needed" @10:19:12.27 Answered recharge if needed"" @10:19:12.28 Vacuuming cartridge 33 @10:19:12.55 Gate.power :heat,:OFF @10:19:12.57 Gate.power :cartridge,:OFF @10:19:12.72 Carousel.in :default @10:19:12.87 Carousel.home.seek 32 @10:19:13.35 Air.in :unlocking @10:19:13.50 Air.seek :clear @10:19:14.53 Lysis1.in :unlocking @10:19:14.74 Lysis1.seek :clear @10:19:14.85 Plunge.in :unlocking @10:19:14.98 Plunge.seek :clear @10:19:16.17 Carousel.seek 33 @10:19:16.75 Lysis1.in :fast @10:19:17.51 Lysis1.seek :locked @10:19:20.22 Plunge.in :default @10:19:20.41 Plunge.seek :locked @10:19:20.58 Air.in :fast @10:19:20.69 Air.seek :locked @10:19:21.70 Carousel.reconfigure :locked @10:19:21.90 Powering cartridge bus @10:19:24.57 Gate.setIR 1750,5000,0,6 @10:19:24.70 Gate.setIR 0,5000,0,6 @10:19:24.82 Gate.power :cartridge,:OFF @10:19:25.41 Mounted archive Cartridge @10:19:25.60 Toroid.in :hold @10:19:25.69 Toroid.seek :cartridge @10:19:27.57 Toroid.in :default @10:19:27.67 Toroid.seek :clear @10:19:29.53 Waiting for bill to answer "record Vacuum" @10:19:34.69 Answered record Vacuum"" @10:19:34.85 Toroid.in :hold @10:19:34.94 Toroid.seek :bypass @10:19:36.62 Toroid.in :default @10:19:36.72 Toroid.seek :clear @10:19:38.42 Waiting for bill to answer "recharge if needed" @10:19:42.04 Answered recharge if needed"" @10:19:42.06 Vacuuming cartridge 34 @10:19:42.43 Carousel.in :default @10:19:42.60 Carousel.home.seek 33 @10:19:43.08 Air.in :unlocking @10:19:43.25 Air.seek :clear @10:19:44.29 Lysis1.in :unlocking @10:19:44.53 Lysis1.seek :clear @10:19:44.67 Plunge.in :unlocking @10:19:44.76 Plunge.seek :clear @10:19:45.95 Carousel.seek 34 @10:19:46.58 Lysis1.in :fast @10:19:46.68 Lysis1.seek :locked @10:19:49.36 Plunge.in :default @10:19:49.50 Plunge.seek :locked @10:19:49.67 Air.in :fast @10:19:49.77 Air.seek :locked @10:19:50.79 Carousel.reconfigure :locked @10:19:51.00 Powering cartridge bus @10:19:53.67 Gate.setIR 1750,5000,0,6 @10:19:53.80 Gate.setIR 0,5000,0,6 @10:19:54.63 PuckHeater.configure :default #ambient 22.0C @10:19:54.73 Gate.power :heat,:ON,limit to 5000mA @10:19:55.44 Mounted lysis Cartridge board#Q05052 @10:19:55.52 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:19:55.68 Toroid.in :hold @10:19:55.77 Toroid.seek :cartridge @10:19:57.67 Toroid.in :default @10:19:57.77 Toroid.seek :clear @10:19:59.63 Waiting for bill to answer "record Vacuum" @10:20:04.49 Answered record Vacuum"" @10:20:04.64 Toroid.in :hold @10:20:04.73 Toroid.seek :bypass @10:20:06.44 Toroid.in :default @10:20:06.55 Toroid.seek :clear @10:20:08.21 Waiting for bill to answer "recharge if needed" @10:20:17.09 Answered recharge if needed"" @10:20:17.11 Vacuuming cartridge 35 @10:20:17.34 Gate.power :heat,:OFF @10:20:17.36 Gate.power :cartridge,:OFF @10:20:17.51 Carousel.in :default @10:20:17.65 Carousel.home.seek 34 @10:20:18.15 Air.in :unlocking @10:20:18.32 Air.seek :clear @10:20:19.40 Lysis1.in :unlocking @10:20:19.59 Lysis1.seek :clear @10:20:19.70 Plunge.in :unlocking @10:20:19.82 Plunge.seek :clear @10:20:21.02 Carousel.seek 35 @10:20:21.61 Lysis1.in :fast @10:20:21.71 Lysis1.seek :locked @10:20:24.42 Plunge.in :default @10:20:24.56 Plunge.seek :locked @10:20:24.74 Air.in :fast @10:20:24.83 Air.seek :locked @10:20:25.85 Carousel.reconfigure :locked @10:20:26.07 Powering cartridge bus @10:20:28.77 Gate.setIR 1750,5000,0,6 @10:20:28.90 Gate.setIR 0,5000,0,6 @10:20:29.02 Gate.power :cartridge,:OFF @10:20:29.61 Mounted archive Cartridge @10:20:29.79 Toroid.in :hold @10:20:29.89 Toroid.seek :cartridge @10:20:31.76 Toroid.in :default @10:20:31.87 Toroid.seek :clear @10:20:33.73 Waiting for bill to answer "record Vacuum" @10:20:38.90 Answered record Vacuum"" @10:20:39.05 Toroid.in :hold @10:20:39.78 Toroid.seek :bypass @10:20:41.50 Toroid.in :default @10:20:41.61 Toroid.seek :clear @10:20:43.33 Waiting for bill to answer "recharge if needed" @10:20:45.19 Answered recharge if needed"" @10:20:45.21 Vacuuming cartridge 36 @10:20:45.53 Carousel.in :default @10:20:45.70 Carousel.home.seek 35 @10:20:46.20 Air.in :unlocking @10:20:46.41 Air.seek :clear @10:20:47.47 Lysis1.in :unlocking @10:20:47.66 Lysis1.seek :clear @10:20:47.74 Plunge.in :unlocking @10:20:47.91 Plunge.seek :clear @10:20:49.16 Carousel.seek 36 @10:20:49.75 Lysis1.in :fast @10:20:49.86 Lysis1.seek :locked @10:20:52.52 Plunge.in :default @10:20:52.61 Plunge.seek :locked @10:20:52.81 Air.in :fast @10:20:52.90 Air.seek :locked @10:20:53.90 Carousel.reconfigure :locked @10:20:54.11 Powering cartridge bus @10:20:56.79 Gate.setIR 1750,5000,0,6 @10:20:56.92 Gate.setIR 0,5000,0,6 @10:20:57.04 PuckHeater.configure :default #ambient 22.0C @10:20:57.15 Gate.power :heat,:ON,limit to 5000mA @10:20:59.85 Mounted lysis Cartridge board#Q05066 @10:20:59.93 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:21:00.10 Toroid.in :hold @10:21:00.21 Toroid.seek :cartridge @10:21:02.09 Toroid.in :default @10:21:02.20 Toroid.seek :clear @10:21:04.03 Waiting for bill to answer "record Vacuum" @10:21:15.88 Answered record Vacuum"" @10:21:16.04 Toroid.in :hold @10:21:16.13 Toroid.seek :bypass @10:21:17.82 Toroid.in :default @10:21:17.93 Toroid.seek :clear @10:21:19.59 Waiting for bill to answer "recharge if needed" @10:21:25.89 Answered recharge if needed"" @10:21:25.91 Vacuuming cartridge 37 @10:21:26.17 Gate.power :heat,:OFF @10:21:26.19 Gate.power :cartridge,:OFF @10:21:26.34 Carousel.in :default @10:21:26.57 Carousel.home.seek 36 @10:21:27.77 Air.in :unlocking @10:21:27.90 Air.seek :clear @10:21:28.94 Lysis1.in :unlocking @10:21:29.15 Lysis1.seek :clear @10:21:29.26 Plunge.in :unlocking @10:21:29.39 Plunge.seek :clear @10:21:30.57 Carousel.seek 37 @10:21:31.17 Lysis1.in :fast @10:21:31.28 Lysis1.seek :locked @10:21:33.95 Plunge.in :default @10:21:34.07 Plunge.seek :locked @10:21:34.26 Air.in :fast @10:21:34.38 Air.seek :locked @10:21:35.40 Carousel.reconfigure :locked @10:21:35.60 Powering cartridge bus @10:21:38.27 Gate.setIR 1750,5000,0,6 @10:21:38.41 Gate.setIR 0,5000,0,6 @10:21:38.52 Gate.power :cartridge,:OFF @10:21:39.10 Mounted archive Cartridge @10:21:39.28 Toroid.in :hold @10:21:39.38 Toroid.seek :cartridge @10:21:41.24 Toroid.in :default @10:21:41.35 Toroid.seek :clear @10:21:43.20 Waiting for bill to answer "record Vacuum" @10:21:48.43 Answered record Vacuum"" @10:21:48.57 Toroid.in :hold @10:21:48.67 Toroid.seek :bypass @10:21:50.36 Toroid.in :default @10:21:50.49 Toroid.seek :clear @10:21:52.14 Waiting for bill to answer "recharge if needed" @10:21:53.90 Answered recharge if needed"" @10:21:53.91 Vacuuming cartridge 38 @10:21:54.24 Carousel.in :default @10:21:54.45 Carousel.home.seek 37 @10:21:54.92 Air.in :unlocking @10:21:55.09 Air.seek :clear @10:21:56.13 Lysis1.in :unlocking @10:21:56.36 Lysis1.seek :clear @10:21:56.43 Plunge.in :unlocking @10:21:56.63 Plunge.seek :clear @10:21:58.53 Carousel.seek 38 @10:21:59.12 Lysis1.in :fast @10:21:59.22 Lysis1.seek :locked @10:22:01.89 Plunge.in :default @10:22:02.01 Plunge.seek :locked @10:22:02.21 Air.in :fast @10:22:02.32 Air.seek :locked @10:22:03.37 Carousel.reconfigure :locked @10:22:03.59 Powering cartridge bus @10:22:06.26 Gate.setIR 1750,5000,0,6 @10:22:06.40 Gate.setIR 0,5000,0,6 @10:22:06.51 PuckHeater.configure :default #ambient 22.0C @10:22:06.62 Gate.power :heat,:ON,limit to 5000mA @10:22:07.40 Mounted lysis Cartridge board#Q05044 @10:22:07.49 Using this cartridge's heat loss curve for HeatingAccelInCarousel @10:22:07.65 Toroid.in :hold @10:22:07.76 Toroid.seek :cartridge @10:22:09.63 Toroid.in :default @10:22:09.74 Toroid.seek :clear @10:22:11.59 Waiting for bill to answer "record Vacuum" @10:22:18.74 Answered record Vacuum"" @10:22:18.88 Toroid.in :hold @10:22:18.98 Toroid.seek :bypass @10:22:20.65 Toroid.in :default @10:22:20.75 Toroid.seek :clear @10:22:22.42 Waiting for bill to answer "recharge if needed" @10:22:29.08 Answered recharge if needed"" @10:22:29.10 Vacuuming cartridge 39 @10:22:29.33 Gate.power :heat,:OFF @10:22:29.36 Gate.power :cartridge,:OFF @10:22:29.51 Carousel.in :default @10:22:29.67 Carousel.home.seek 38 @10:22:30.14 Air.in :unlocking @10:22:30.33 Air.seek :clear @10:22:31.43 Lysis1.in :unlocking @10:22:31.57 Lysis1.seek :clear @10:22:31.78 Plunge.in :unlocking @10:22:31.90 Plunge.seek :clear @10:22:33.05 Carousel.seek 39 @10:22:33.63 Lysis1.in :fast @10:22:33.74 Lysis1.seek :locked @10:22:36.45 Plunge.in :default @10:22:36.56 Plunge.seek :locked @10:22:37.39 Air.in :fast @10:22:37.51 Air.seek :locked @10:22:38.52 Carousel.reconfigure :locked @10:22:38.76 Powering cartridge bus @10:22:41.45 Gate.setIR 1750,5000,0,6 @10:22:41.59 Gate.setIR 0,5000,0,6 @10:22:41.71 Gate.power :cartridge,:OFF @10:22:42.31 Mounted archive Cartridge @10:22:42.52 Toroid.in :hold @10:22:42.62 Toroid.seek :cartridge @10:22:44.51 Toroid.in :default @10:22:44.61 Toroid.seek :clear @10:22:46.47 Waiting for bill to answer "record Vacuum" @10:22:52.63 Answered record Vacuum"" @10:22:52.80 Toroid.in :hold @10:22:52.90 Toroid.seek :bypass @10:22:54.59 Toroid.in :default @10:22:54.69 Toroid.seek :clear @10:22:56.40 Waiting for bill to answer "recharge if needed" @10:22:58.36 Answered recharge if needed"" @10:22:58.39 Vacuuming cartridge 40 @10:22:58.72 Carousel.in :default @10:22:58.89 Carousel.home.seek 39 @10:22:59.35 Air.in :unlocking @10:22:59.54 Air.seek :clear @10:23:00.59 Lysis1.in :unlocking @10:23:00.79 Lysis1.seek :clear @10:23:00.86 Plunge.in :unlocking @10:23:01.03 Plunge.seek :clear @10:23:02.27 Carousel.seek 40 @10:23:02.88 Lysis1.in :fast @10:23:02.97 Lysis1.seek :locked @10:23:05.65 Plunge.in :default @10:23:05.79 Plunge.seek :locked @10:23:05.93 Air.in :fast @10:23:06.00 Air.seek :locked @10:23:07.02 Carousel.reconfigure :locked @10:23:07.22 Powering cartridge bus @10:23:09.89 Gate.setIR 1750,5000,0,6 @10:23:10.03 Gate.setIR 0,5000,0,6 @10:23:10.14 PuckHeater.configure :default #ambient 22.0C @10:23:10.26 Gate.power :heat,:ON,limit to 5000mA @10:23:10.97 Mounted lysis Cartridge board#Q05041 @10:23:11.05 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:23:11.88 Toroid.in :hold @10:23:12.01 Toroid.seek :cartridge @10:23:13.90 Toroid.in :default @10:23:14.00 Toroid.seek :clear @10:23:15.86 Waiting for bill to answer "record Vacuum" @10:23:22.33 Answered record Vacuum"" @10:23:22.53 Toroid.in :hold @10:23:22.62 Toroid.seek :bypass @10:23:24.30 Toroid.in :default @10:23:24.43 Toroid.seek :clear @10:23:26.09 Waiting for bill to answer "recharge if needed" @10:23:33.90 Answered recharge if needed"" @10:23:33.92 Vacuuming cartridge 41 @10:23:34.15 Gate.power :heat,:OFF @10:23:34.18 Gate.power :cartridge,:OFF @10:23:34.33 Carousel.in :default @10:23:34.53 Carousel.home.seek 40 @10:23:35.02 Air.in :unlocking @10:23:35.22 Air.seek :clear @10:23:36.29 Lysis1.in :unlocking @10:23:36.48 Lysis1.seek :clear @10:23:36.64 Plunge.in :unlocking @10:23:36.75 Plunge.seek :clear @10:23:37.90 Carousel.seek 41 @10:23:38.52 Lysis1.in :fast @10:23:38.63 Lysis1.seek :locked @10:23:41.30 Plunge.in :default @10:23:41.39 Plunge.seek :locked @10:23:41.61 Air.in :fast @10:23:41.73 Air.seek :locked @10:23:42.75 Carousel.reconfigure :locked @10:23:42.96 Powering cartridge bus @10:23:45.63 Gate.setIR 1750,5000,0,6 @10:23:45.77 Gate.setIR 0,5000,0,6 @10:23:45.88 Gate.power :cartridge,:OFF @10:23:46.47 Mounted archive Cartridge @10:23:46.66 Toroid.in :hold @10:23:46.75 Toroid.seek :cartridge @10:23:48.64 Toroid.in :default @10:23:48.74 Toroid.seek :clear @10:23:50.59 Waiting for bill to answer "record Vacuum" @10:23:57.45 Answered record Vacuum"" @10:23:57.60 Toroid.in :hold @10:23:57.70 Toroid.seek :bypass @10:23:59.38 Toroid.in :default @10:23:59.48 Toroid.seek :clear @10:24:01.15 Waiting for bill to answer "recharge if needed" @10:24:06.46 Answered recharge if needed"" @10:24:06.52 Vacuuming cartridge 42 @10:24:06.82 Carousel.in :default @10:24:06.99 Carousel.home.seek 41 @10:24:07.47 Air.in :unlocking @10:24:07.63 Air.seek :clear @10:24:08.70 Lysis1.in :unlocking @10:24:08.88 Lysis1.seek :clear @10:24:08.99 Plunge.in :unlocking @10:24:09.10 Plunge.seek :clear @10:24:10.30 Carousel.seek 42 @10:24:10.92 Lysis1.in :fast @10:24:11.02 Lysis1.seek :locked @10:24:13.68 Plunge.in :default @10:24:13.80 Plunge.seek :locked @10:24:13.99 Air.in :fast @10:24:14.09 Air.seek :locked @10:24:15.10 Carousel.reconfigure :locked @10:24:15.31 Powering cartridge bus @10:24:17.98 Gate.setIR 1750,5000,0,6 @10:24:18.11 Gate.setIR 0,5000,0,6 @10:24:18.22 PuckHeater.configure :default #ambient 22.0C @10:24:18.33 Gate.power :heat,:ON,limit to 5000mA @10:24:19.11 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @10:24:19.19 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:24:19.35 Toroid.in :hold @10:24:19.45 Toroid.seek :cartridge @10:24:21.33 Toroid.in :default @10:24:21.44 Toroid.seek :clear @10:24:23.30 Waiting for bill to answer "record Vacuum" @10:24:27.04 Answered record Vacuum"" @10:24:27.19 Toroid.in :hold @10:24:27.28 Toroid.seek :bypass @10:24:28.95 Toroid.in :default @10:24:29.05 Toroid.seek :clear @10:24:30.71 Waiting for bill to answer "recharge if needed" @10:24:46.70 Answered recharge if needed"" @10:24:46.77 Vacuuming cartridge 43 @10:24:47.01 Gate.power :heat,:OFF @10:24:47.04 Gate.power :cartridge,:OFF @10:24:47.25 Carousel.in :default @10:24:47.43 Carousel.home.seek 42 @10:24:47.90 Air.in :unlocking @10:24:48.73 Air.seek :clear @10:24:49.80 Lysis1.in :unlocking @10:24:49.99 Lysis1.seek :clear @10:24:50.10 Plunge.in :unlocking @10:24:50.23 Plunge.seek :clear @10:24:51.41 Carousel.seek 43 @10:24:52.00 Lysis1.in :fast @10:24:52.11 Lysis1.seek :locked @10:24:54.78 Plunge.in :default @10:24:54.87 Plunge.seek :locked @10:24:55.07 Air.in :fast @10:24:55.17 Air.seek :locked @10:24:56.19 Carousel.reconfigure :locked @10:24:56.42 Powering cartridge bus @10:24:59.09 Gate.setIR 1750,5000,0,6 @10:24:59.22 Gate.setIR 0,5000,0,6 @10:24:59.34 Gate.power :cartridge,:OFF @10:24:59.93 Mounted archive Cartridge @10:25:00.11 Toroid.in :hold @10:25:00.21 Toroid.seek :cartridge @10:25:02.10 Toroid.in :default @10:25:02.20 Toroid.seek :clear @10:25:04.04 Waiting for bill to answer "record Vacuum" @10:25:13.13 Answered record Vacuum"" @10:25:13.29 Toroid.in :hold @10:25:13.39 Toroid.seek :bypass @10:25:15.08 Toroid.in :default @10:25:15.18 Toroid.seek :clear @10:25:16.84 Waiting for bill to answer "recharge if needed" @10:25:33.91 Answered recharge if needed"" @10:25:33.93 Vacuuming cartridge 44 @10:25:34.22 Carousel.in :default @10:25:34.42 Carousel.home.seek 43 @10:25:34.90 Air.in :unlocking @10:25:35.08 Air.seek :clear @10:25:36.13 Lysis1.in :unlocking @10:25:36.28 Lysis1.seek :clear @10:25:36.49 Plunge.in :unlocking @10:25:36.59 Plunge.seek :clear @10:25:37.75 Carousel.seek 44 @10:25:38.33 Lysis1.in :fast @10:25:38.48 Lysis1.seek :locked @10:25:41.85 Plunge.in :default @10:25:41.96 Plunge.seek :locked @10:25:42.16 Air.in :fast @10:25:42.26 Air.seek :locked @10:25:43.28 Carousel.reconfigure :locked @10:25:43.48 Powering cartridge bus @10:25:46.15 Gate.setIR 1750,5000,0,6 @10:25:46.28 Gate.setIR 0,5000,0,6 @10:25:46.42 PuckHeater.configure :default #ambient 22.0C @10:25:46.53 Gate.power :heat,:ON,limit to 5000mA @10:25:47.23 Mounted lysis Cartridge board#Q05065 @10:25:47.32 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:25:47.49 Toroid.in :hold @10:25:47.59 Toroid.seek :cartridge @10:25:49.47 Toroid.in :default @10:25:49.58 Toroid.seek :clear @10:25:51.43 Waiting for bill to answer "record Vacuum" @10:25:55.62 Answered record Vacuum"" @10:25:55.78 Toroid.in :hold @10:25:55.88 Toroid.seek :bypass @10:25:57.59 Toroid.in :default @10:25:57.70 Toroid.seek :clear @10:25:59.35 Waiting for bill to answer "recharge if needed" @10:26:08.73 Answered recharge if needed"" @10:26:08.74 Vacuuming cartridge 45 @10:26:08.97 Gate.power :heat,:OFF @10:26:09.00 Gate.power :cartridge,:OFF @10:26:09.16 Carousel.in :default @10:26:09.31 Carousel.home.seek 44 @10:26:09.78 Air.in :unlocking @10:26:09.92 Air.seek :clear @10:26:10.98 Lysis1.in :unlocking @10:26:11.14 Lysis1.seek :clear @10:26:11.30 Plunge.in :unlocking @10:26:11.39 Plunge.seek :clear @10:26:12.55 Carousel.seek 45 @10:26:13.13 Lysis1.in :fast @10:26:13.24 Lysis1.seek :locked @10:26:15.90 Plunge.in :default @10:26:16.05 Plunge.seek :locked @10:26:16.19 Air.in :fast @10:26:16.29 Air.seek :locked @10:26:17.34 Carousel.reconfigure :locked @10:26:17.53 Powering cartridge bus @10:26:20.10 Gate.setIR 1750,5000,0,6 @10:26:20.26 Gate.setIR 0,5000,0,6 @10:26:20.43 Gate.power :cartridge,:OFF @10:26:21.02 Mounted archive Cartridge @10:26:21.22 Toroid.in :hold @10:26:21.34 Toroid.seek :cartridge @10:26:23.22 Toroid.in :default @10:26:23.33 Toroid.seek :clear @10:26:25.19 Waiting for bill to answer "record Vacuum" @10:26:28.80 Answered record Vacuum"" @10:26:28.97 Toroid.in :hold @10:26:29.07 Toroid.seek :bypass @10:26:30.76 Toroid.in :default @10:26:30.86 Toroid.seek :clear @10:26:32.51 Waiting for bill to answer "recharge if needed" @10:26:36.16 Answered recharge if needed"" @10:26:36.17 Vacuuming cartridge 46 @10:26:36.52 Carousel.in :default @10:26:36.68 Carousel.home.seek 45 @10:26:37.16 Air.in :unlocking @10:26:37.31 Air.seek :clear @10:26:38.36 Lysis1.in :unlocking @10:26:38.67 Lysis1.seek :clear @10:26:38.74 Plunge.in :unlocking @10:26:38.95 Plunge.seek :clear @10:26:40.20 Carousel.seek 46 @10:26:40.79 Lysis1.in :fast @10:26:40.90 Lysis1.seek :locked @10:26:43.57 Plunge.in :default @10:26:43.66 Plunge.seek :locked @10:26:43.87 Air.in :fast @10:26:43.98 Air.seek :locked @10:26:45.00 Carousel.reconfigure :locked @10:26:45.20 Powering cartridge bus @10:26:47.87 Gate.setIR 1750,5000,0,6 @10:26:48.00 Gate.setIR 0,5000,0,6 @10:26:48.16 PuckHeater.configure :default #ambient 22.0C @10:26:48.28 Gate.power :heat,:ON,limit to 5000mA @10:26:48.99 Mounted lysis Cartridge board#Q05046 @10:26:49.08 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:26:49.25 Toroid.in :hold @10:26:49.34 Toroid.seek :cartridge @10:26:51.23 Toroid.in :default @10:26:51.32 Toroid.seek :clear @10:26:53.18 Waiting for bill to answer "record Vacuum" @10:26:58.29 Answered record Vacuum"" @10:26:58.49 Toroid.in :hold @10:26:58.59 Toroid.seek :bypass @10:27:00.28 Toroid.in :default @10:27:00.41 Toroid.seek :clear @10:27:02.05 Waiting for bill to answer "recharge if needed" @10:27:13.14 Answered recharge if needed"" @10:27:13.15 Vacuuming cartridge 47 @10:27:13.39 Gate.power :heat,:OFF @10:27:13.44 Gate.power :cartridge,:OFF @10:27:13.57 Carousel.in :default @10:27:13.73 Carousel.home.seek 46 @10:27:14.21 Air.in :unlocking @10:27:14.32 Air.seek :clear @10:27:15.47 Lysis1.in :unlocking @10:27:15.64 Lysis1.seek :clear @10:27:15.72 Plunge.in :unlocking @10:27:15.89 Plunge.seek :clear @10:27:17.12 Carousel.seek 47 @10:27:17.72 Lysis1.in :fast @10:27:17.82 Lysis1.seek :locked @10:27:20.52 Plunge.in :default @10:27:20.62 Plunge.seek :locked @10:27:20.84 Air.in :fast @10:27:20.94 Air.seek :locked @10:27:21.95 Carousel.reconfigure :locked @10:27:22.15 Powering cartridge bus @10:27:24.82 Gate.setIR 1750,5000,0,6 @10:27:24.95 Gate.setIR 0,5000,0,6 @10:27:25.07 Gate.power :cartridge,:OFF @10:27:25.66 Mounted archive Cartridge @10:27:25.86 Toroid.in :hold @10:27:25.97 Toroid.seek :cartridge @10:27:27.85 Toroid.in :default @10:27:27.96 Toroid.seek :clear @10:27:29.82 Waiting for bill to answer "record Vacuum" @10:27:33.21 Answered record Vacuum"" @10:27:33.38 Toroid.in :hold @10:27:33.48 Toroid.seek :bypass @10:27:35.17 Toroid.in :default @10:27:35.26 Toroid.seek :clear @10:27:36.92 Waiting for bill to answer "recharge if needed" @10:27:40.99 Answered recharge if needed"" @10:27:41.01 Vacuuming cartridge 48 @10:27:41.33 Carousel.in :default @10:27:41.50 Carousel.home.seek 47 @10:27:42.65 Air.in :unlocking @10:27:42.81 Air.seek :clear @10:27:43.84 Lysis1.in :unlocking @10:27:44.00 Lysis1.seek :clear @10:27:44.16 Plunge.in :unlocking @10:27:44.27 Plunge.seek :clear @10:27:45.42 Carousel.seek 48 @10:27:46.00 Lysis1.in :fast @10:27:46.11 Lysis1.seek :locked @10:27:48.79 Plunge.in :default @10:27:48.88 Plunge.seek :locked @10:27:49.09 Air.in :fast @10:27:49.20 Air.seek :locked @10:27:50.23 Carousel.reconfigure :locked @10:27:50.47 Powering cartridge bus @10:27:53.14 Gate.setIR 1750,5000,0,6 @10:27:53.28 Gate.setIR 0,5000,0,6 @10:27:53.38 PuckHeater.configure :default #ambient 22.0C @10:27:53.50 Gate.power :heat,:ON,limit to 5000mA @10:27:54.19 Mounted lysis Cartridge board#Q05062 @10:27:54.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:27:54.48 Toroid.in :hold @10:27:54.59 Toroid.seek :cartridge @10:27:56.47 Toroid.in :default @10:27:56.58 Toroid.seek :clear @10:27:58.44 Waiting for bill to answer "record Vacuum" @10:28:03.21 Answered record Vacuum"" @10:28:03.37 Toroid.in :hold @10:28:03.47 Toroid.seek :bypass @10:28:05.15 Toroid.in :default @10:28:05.26 Toroid.seek :clear @10:28:06.91 Waiting for bill to answer "recharge if needed" @10:28:14.88 Answered recharge if needed"" @10:28:14.90 Vacuuming cartridge 49 @10:28:15.13 Gate.power :heat,:OFF @10:28:15.16 Gate.power :cartridge,:OFF @10:28:15.29 Carousel.in :default @10:28:15.45 Carousel.home.seek 48 @10:28:15.93 Air.in :unlocking @10:28:16.10 Air.seek :clear @10:28:17.15 Lysis1.in :unlocking @10:28:17.31 Lysis1.seek :clear @10:28:17.47 Plunge.in :unlocking @10:28:18.24 Plunge.seek :clear @10:28:19.39 Carousel.seek 49 @10:28:20.00 Lysis1.in :fast @10:28:20.11 Lysis1.seek :locked @10:28:22.78 Plunge.in :default @10:28:22.94 Plunge.seek :locked @10:28:23.10 Air.in :fast @10:28:23.18 Air.seek :locked @10:28:24.18 Carousel.reconfigure :locked @10:28:24.42 Powering cartridge bus @10:28:27.09 Gate.setIR 1750,5000,0,6 @10:28:27.22 Gate.setIR 0,5000,0,6 @10:28:27.34 Gate.power :cartridge,:OFF @10:28:27.93 Mounted archive Cartridge @10:28:28.11 Toroid.in :hold @10:28:28.21 Toroid.seek :cartridge @10:28:30.09 Toroid.in :default @10:28:30.20 Toroid.seek :clear @10:28:32.06 Waiting for bill to answer "record Vacuum" @10:28:35.30 Answered record Vacuum"" @10:28:35.46 Toroid.in :hold @10:28:35.57 Toroid.seek :bypass @10:28:37.25 Toroid.in :default @10:28:37.34 Toroid.seek :clear @10:28:39.00 Waiting for bill to answer "recharge if needed" @10:28:43.05 Answered recharge if needed"" @10:28:43.06 Vacuuming cartridge 50 @10:28:43.36 Carousel.in :default @10:28:43.53 Carousel.home.seek 49 @10:28:44.00 Air.in :unlocking @10:28:44.17 Air.seek :clear @10:28:45.23 Lysis1.in :unlocking @10:28:45.41 Lysis1.seek :clear @10:28:45.57 Plunge.in :unlocking @10:28:45.67 Plunge.seek :clear @10:28:46.83 Carousel.seek 50 @10:28:47.41 Lysis1.in :fast @10:28:47.52 Lysis1.seek :locked @10:28:50.17 Plunge.in :default @10:28:50.32 Plunge.seek :locked @10:28:50.50 Air.in :fast @10:28:50.59 Air.seek :locked @10:28:52.27 Carousel.reconfigure :locked @10:28:52.49 Powering cartridge bus @10:28:55.17 Gate.setIR 1750,5000,0,6 @10:28:55.31 Gate.setIR 0,5000,0,6 @10:28:55.42 PuckHeater.configure :default #ambient 22.0C @10:28:55.54 Gate.power :heat,:ON,limit to 5000mA @10:28:56.23 Mounted lysis Cartridge board#Q05053 @10:28:56.32 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:28:56.52 Toroid.in :hold @10:28:56.62 Toroid.seek :cartridge @10:28:58.50 Toroid.in :default @10:28:58.60 Toroid.seek :clear @10:29:00.47 Waiting for bill to answer "record Vacuum" @10:29:05.27 Answered record Vacuum"" @10:29:05.41 Toroid.in :hold @10:29:05.51 Toroid.seek :bypass @10:29:07.19 Toroid.in :default @10:29:07.29 Toroid.seek :clear @10:29:08.96 Waiting for bill to answer "recharge if needed" @10:29:17.55 Answered recharge if needed"" @10:29:17.57 Vacuuming cartridge 51 @10:29:17.81 Gate.power :heat,:OFF @10:29:17.84 Gate.power :cartridge,:OFF @10:29:17.98 Carousel.in :default @10:29:18.14 Carousel.home.seek 50 @10:29:18.66 Air.in :unlocking @10:29:18.85 Air.seek :clear @10:29:19.92 Lysis1.in :unlocking @10:29:20.08 Lysis1.seek :clear @10:29:20.24 Plunge.in :unlocking @10:29:20.38 Plunge.seek :clear @10:29:21.53 Carousel.seek 51 @10:29:22.12 Lysis1.in :fast @10:29:22.23 Lysis1.seek :locked @10:29:24.89 Plunge.in :default @10:29:24.97 Plunge.seek :locked @10:29:25.17 Air.in :fast @10:29:25.27 Air.seek :locked @10:29:26.28 Carousel.reconfigure :locked @10:29:26.52 Powering cartridge bus @10:29:29.19 Gate.setIR 1750,5000,0,6 @10:29:29.32 Gate.setIR 0,5000,0,6 @10:29:29.44 Gate.power :cartridge,:OFF @10:29:30.03 Mounted archive Cartridge @10:29:30.22 Toroid.in :hold @10:29:31.02 Toroid.seek :cartridge @10:29:32.93 Toroid.in :default @10:29:33.04 Toroid.seek :clear @10:29:34.89 Waiting for bill to answer "record Vacuum" @10:29:38.06 Answered record Vacuum"" @10:29:38.23 Toroid.in :hold @10:29:38.36 Toroid.seek :bypass @10:29:40.06 Toroid.in :default @10:29:40.17 Toroid.seek :clear @10:29:41.84 Waiting for bill to answer "recharge if needed" @10:29:46.18 Answered recharge if needed"" @10:29:46.20 Vacuuming cartridge 56 @10:29:46.54 Carousel.in :default @10:29:46.71 Carousel.home.seek 51 @10:29:47.18 Air.in :unlocking @10:29:47.33 Air.seek :clear @10:29:48.41 Lysis1.in :unlocking @10:29:48.59 Lysis1.seek :clear @10:29:48.71 Plunge.in :unlocking @10:29:48.83 Plunge.seek :clear @10:29:50.02 Carousel.seek 56 @10:29:51.45 Lysis1.in :fast @10:29:51.55 Lysis1.seek :locked @10:29:54.22 Plunge.in :default @10:29:54.39 Plunge.seek :locked @10:29:54.57 Air.in :fast @10:29:54.66 Air.seek :locked @10:29:55.69 Carousel.reconfigure :locked @10:29:55.89 Powering cartridge bus @10:29:58.56 Gate.setIR 1750,5000,0,6 @10:29:58.69 Gate.setIR 0,5000,0,6 @10:29:58.81 Gate.power :cartridge,:OFF @10:29:59.40 Mounted archive Cartridge @10:29:59.59 Toroid.in :hold @10:29:59.69 Toroid.seek :cartridge @10:30:01.58 Toroid.in :default @10:30:01.69 Toroid.seek :clear @10:30:03.54 Waiting for bill to answer "record Vacuum" @10:30:10.70 Answered record Vacuum"" @10:30:10.84 Toroid.in :hold @10:30:10.94 Toroid.seek :bypass @10:30:12.62 Toroid.in :default @10:30:12.72 Toroid.seek :clear @10:30:14.42 Waiting for bill to answer "recharge if needed" @10:30:21.25 Answered recharge if needed"" @10:30:21.27 Vacuuming cartridge 57 @10:30:22.20 Carousel.in :default @10:30:22.44 Carousel.home.seek 56 @10:30:23.00 Air.in :unlocking @10:30:23.20 Air.seek :clear @10:30:24.26 Lysis1.in :unlocking @10:30:24.48 Lysis1.seek :clear @10:30:24.54 Plunge.in :unlocking @10:30:24.72 Plunge.seek :clear @10:30:25.98 Carousel.seek 57 @10:30:26.61 Lysis1.in :fast @10:30:26.71 Lysis1.seek :locked @10:30:29.39 Plunge.in :default @10:30:29.56 Plunge.seek :locked @10:30:29.70 Air.in :fast @10:30:29.80 Air.seek :locked @10:30:30.81 Carousel.reconfigure :locked @10:30:31.02 Powering cartridge bus @10:30:33.69 Gate.setIR 1750,5000,0,6 @10:30:33.82 Gate.setIR 0,5000,0,6 @10:30:33.93 PuckHeater.configure :default #ambient 22.0C @10:30:34.03 Gate.power :heat,:ON,limit to 5000mA @10:30:34.72 Mounted lysis Cartridge board#Q05075 @10:30:34.79 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:30:34.96 Toroid.in :hold @10:30:35.05 Toroid.seek :cartridge @10:30:36.93 Toroid.in :default @10:30:37.04 Toroid.seek :clear @10:30:38.87 Waiting for bill to answer "record Vacuum" @10:30:41.85 Answered record Vacuum"" @10:30:42.00 Toroid.in :hold @10:30:42.11 Toroid.seek :bypass @10:30:43.80 Toroid.in :default @10:30:43.89 Toroid.seek :clear @10:30:45.56 Waiting for bill to answer "recharge if needed" @10:31:01.49 Answered recharge if needed"" @10:31:01.50 Vacuuming cartridge 58 @10:31:01.73 Gate.power :heat,:OFF @10:31:01.76 Gate.power :cartridge,:OFF @10:31:01.91 Carousel.in :default @10:31:02.06 Carousel.home.seek 57 @10:31:02.59 Air.in :unlocking @10:31:02.76 Air.seek :clear @10:31:04.50 Lysis1.in :unlocking @10:31:04.66 Lysis1.seek :clear @10:31:04.81 Plunge.in :unlocking @10:31:04.92 Plunge.seek :clear @10:31:06.06 Carousel.seek 58 @10:31:06.66 Lysis1.in :fast @10:31:06.77 Lysis1.seek :locked @10:31:09.44 Plunge.in :default @10:31:09.58 Plunge.seek :locked @10:31:09.72 Air.in :fast @10:31:09.82 Air.seek :locked @10:31:10.83 Carousel.reconfigure :locked @10:31:11.02 Powering cartridge bus @10:31:13.70 Gate.setIR 1750,5000,0,6 @10:31:13.84 Gate.setIR 0,5000,0,6 @10:31:13.96 Gate.power :cartridge,:OFF @10:31:14.55 Mounted archive Cartridge @10:31:14.74 Toroid.in :hold @10:31:14.84 Toroid.seek :cartridge @10:31:16.74 Toroid.in :default @10:31:16.85 Toroid.seek :clear @10:31:18.67 Waiting for bill to answer "record Vacuum" @10:31:21.75 Answered record Vacuum"" @10:31:21.93 Toroid.in :hold @10:31:22.04 Toroid.seek :bypass @10:31:23.72 Toroid.in :default @10:31:23.82 Toroid.seek :clear @10:31:25.48 Waiting for bill to answer "recharge if needed" @10:31:31.90 Answered recharge if needed"" @10:31:31.92 Vacuuming cartridge 59 @10:31:32.23 Carousel.in :default @10:31:32.44 Carousel.home.seek 58 @10:31:32.91 Air.in :unlocking @10:31:33.07 Air.seek :clear @10:31:34.11 Lysis1.in :unlocking @10:31:34.27 Lysis1.seek :clear @10:31:34.44 Plunge.in :unlocking @10:31:34.53 Plunge.seek :clear @10:31:35.68 Carousel.seek 59 @10:31:36.27 Lysis1.in :fast @10:31:36.42 Lysis1.seek :locked @10:31:39.73 Plunge.in :default @10:31:39.91 Plunge.seek :locked @10:31:40.08 Air.in :fast @10:31:40.18 Air.seek :locked @10:31:41.20 Carousel.reconfigure :locked @10:31:41.41 Powering cartridge bus @10:31:44.09 Gate.setIR 1750,5000,0,6 @10:31:44.22 Gate.setIR 0,5000,0,6 @10:31:44.33 PuckHeater.configure :default #ambient 22.0C @10:31:44.47 Gate.power :heat,:ON,limit to 5000mA @10:31:45.18 Mounted lysis Cartridge board#Q05086 @10:31:45.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:31:45.42 Toroid.in :hold @10:31:45.52 Toroid.seek :cartridge @10:31:47.39 Toroid.in :default @10:31:47.49 Toroid.seek :clear @10:31:49.34 Waiting for bill to answer "record Vacuum" @10:31:54.43 Answered record Vacuum"" @10:31:54.58 Toroid.in :hold @10:31:54.69 Toroid.seek :bypass @10:31:56.41 Toroid.in :default @10:31:56.51 Toroid.seek :clear @10:31:58.18 Waiting for bill to answer "recharge if needed" @10:32:07.13 Answered recharge if needed"" @10:32:07.15 Vacuuming cartridge 60 @10:32:07.40 Gate.power :heat,:OFF @10:32:07.43 Gate.power :cartridge,:OFF @10:32:07.56 Carousel.in :default @10:32:07.73 Carousel.home.seek 59 @10:32:08.20 Air.in :unlocking @10:32:08.42 Air.seek :clear @10:32:09.49 Lysis1.in :unlocking @10:32:09.69 Lysis1.seek :clear @10:32:09.74 Plunge.in :unlocking @10:32:09.92 Plunge.seek :clear @10:32:11.16 Carousel.seek 60 @10:32:11.77 Lysis1.in :fast @10:32:11.88 Lysis1.seek :locked @10:32:14.54 Plunge.in :default @10:32:14.65 Plunge.seek :locked @10:32:14.84 Air.in :fast @10:32:14.93 Air.seek :locked @10:32:15.93 Carousel.reconfigure :locked @10:32:16.14 Powering cartridge bus @10:32:19.43 Gate.setIR 1750,5000,0,6 @10:32:19.61 Gate.setIR 0,5000,0,6 @10:32:19.72 PuckHeater.configure :default #ambient 22.0C @10:32:19.84 Gate.power :heat,:ON,limit to 5000mA @10:32:20.55 Mounted lysis Cartridge board#Q05051 @10:32:20.64 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:32:20.82 Toroid.in :hold @10:32:20.93 Toroid.seek :cartridge @10:32:22.80 Toroid.in :default @10:32:22.91 Toroid.seek :clear @10:32:24.76 Waiting for bill to answer "record Vacuum" @10:32:33.75 Answered record Vacuum"" @10:32:33.91 Toroid.in :hold @10:32:34.00 Toroid.seek :bypass @10:32:35.69 Toroid.in :default @10:32:35.79 Toroid.seek :clear @10:32:37.46 Waiting for bill to answer "recharge if needed" @10:32:43.89 Answered recharge if needed"" @10:32:43.95 Gate.power :heat,:OFF @10:32:43.98 Gate.power :cartridge,:OFF @10:32:44.11 Carousel.in :default @10:32:44.26 Carousel.home.seek 60 @10:32:44.72 Air.in :unlocking @10:32:44.90 Air.seek :clear @10:32:45.96 Lysis1.in :unlocking @10:32:46.13 Lysis1.seek :clear @10:32:46.20 Plunge.in :unlocking @10:32:46.41 Plunge.seek :clear @10:32:47.62 Waiting for bill to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @10:32:56.67 UserAbort in bill -- User Abort!! @10:33:27.60 -> SC 2 @10:33:27.63 NoMethodError in bill -- undefined method `SC' for CmdServer::Client:Class @10:33:37.58 -> SC.to 2 @10:33:37.62 Carousel.seek 2 Storage Carousel (SC) at 2 @10:35:59.99 -> SC.to 16 @10:36:00.03 Carousel.seek 16 Storage Carousel (SC) at 16 @10:40:05.83 -> SC.to 18 @10:40:05.87 Carousel.seek 18 Storage Carousel (SC) at 18 @10:42:54.52 -> SC.to 28 @10:42:54.56 Carousel.seek 28 Storage Carousel (SC) at 28 @10:51:47.80 -> SC.to 30 @10:51:47.85 Carousel.seek 30 Storage Carousel (SC) at 30 @10:55:04.92 -> slot {[60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :lyseHold_phyto, State: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archive_bac, State: :dry}, 55..52 => { Type: :unknown, State: :missing}} @10:55:20.56 -> slot [60..1] =>ni @10:55:20.61 Cartridge::InvalidTypeOrState in bill -- :ni is neither type nor state @10:55:25.49 -> slot [60..1] =>nil {60..1 => nil} @10:56:11.17 -> slot [30, 28, 18, 16, 2] =>[:dry] {[30,28,18,16,2] => { Type: :unknown, State: :dry}} @10:56:29.40 -> vacToroid @10:56:29.46 Vacuuming protocol for dry cartridges @10:56:30.28 Toroid.seek :clear @10:56:31.24 Carousel.in :default @10:56:31.41 Carousel.home.seek 30 @10:56:31.74 Waiting for bill to answer "Disconnect the intake line. push return" @10:56:36.66 Answered Disconnect the intake line. push return"" @10:56:37.47 Toroid.seek :clear @10:56:37.70 Carousel.in :default @10:56:37.86 Carousel.home.seek 30 @10:56:38.18 SamplePump.setPosition! 0ml @10:56:38.26 Intake.open @10:56:38.96 SamplePump.seek 50ml @10:57:40.61 Exhaust.close @10:57:41.28 SamplePump.seek 75ml @10:58:12.41 Intake.close @10:58:13.13 Bypass.close @10:58:13.86 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @10:58:29.17 UserAbort in bill -- User Abort!! @10:58:34.48 -> vacToroid @10:58:34.55 Vacuuming protocol for dry cartridges @10:58:35.39 Toroid.seek :clear @10:58:35.63 Carousel.in :default @10:58:35.80 Carousel.home.seek 30 @10:58:36.12 Waiting for bill to answer "Disconnect the intake line. push return" @10:58:42.11 Answered Disconnect the intake line. push return"" @10:58:42.96 Toroid.seek :clear @10:58:43.18 Carousel.in :default @10:58:43.32 Carousel.home.seek 30 @10:58:43.63 SamplePump.setPosition! 0ml @10:58:43.72 Intake.open @10:58:44.45 Exhaust.open @10:58:45.11 SamplePump.seek 50ml @10:59:46.75 Exhaust.close @10:59:47.43 Bypass.open @10:59:48.16 SamplePump.seek 75ml @11:00:19.29 Intake.close @11:00:20.03 Bypass.close @11:00:20.75 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @11:00:30.96 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @11:00:30.98 Exhaust.open @11:00:31.70 Bypass.open @11:00:33.08 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @11:01:51.87 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @11:01:51.90 delay 30 seconds @11:02:21.96 Clearing fluid from cartridge-side of Toroid @11:02:22.30 Carousel.seek 2 @11:02:28.27 Toroid.in :hold @11:02:28.40 Toroid.seek :cartridge @11:02:30.27 Toroid.in :default @11:02:30.41 Toroid.seek :clear @11:02:32.24 delay 2 seconds @11:02:34.37 Toroid.in :hold @11:02:34.49 Toroid.seek :bypass @11:02:36.19 Toroid.in :default @11:02:36.30 Toroid.seek :clear @11:02:38.29 Carousel.seek 16 @11:02:41.43 Toroid.in :hold @11:02:41.54 Toroid.seek :cartridge @11:02:43.41 Toroid.in :default @11:02:43.50 Toroid.seek :clear @11:02:45.35 delay 2 seconds @11:02:47.47 Toroid.in :hold @11:02:47.57 Toroid.seek :bypass @11:02:49.24 Toroid.in :default @11:02:49.35 Toroid.seek :clear @11:02:51.28 Carousel.seek 18 @11:02:51.94 Toroid.in :hold @11:02:52.04 Toroid.seek :cartridge @11:02:53.91 Toroid.in :default @11:02:54.02 Toroid.seek :clear @11:02:55.86 delay 2 seconds @11:02:57.97 Toroid.in :hold @11:02:58.07 Toroid.seek :bypass @11:02:59.75 Toroid.in :default @11:02:59.85 Toroid.seek :clear @11:03:01.78 Carousel.seek 28 @11:03:04.07 Toroid.in :hold @11:03:04.17 Toroid.seek :cartridge @11:03:06.10 Toroid.in :default @11:03:06.20 Toroid.seek :clear @11:03:08.04 delay 2 seconds @11:03:10.16 Toroid.in :hold @11:03:10.27 Toroid.seek :bypass @11:03:11.94 Toroid.in :default @11:03:12.04 Toroid.seek :clear @11:03:14.69 Carousel.seek 30 @11:03:15.36 Toroid.in :hold @11:03:15.46 Toroid.seek :cartridge @11:03:17.34 Toroid.in :default @11:03:17.44 Toroid.seek :clear @11:03:19.28 delay 2 seconds @11:03:21.41 Toroid.in :hold @11:03:21.52 Toroid.seek :bypass @11:03:23.18 Toroid.in :default @11:03:23.28 Toroid.seek :clear @11:03:24.94 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @11:03:31.08 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @11:03:31.90 Toroid.seek :clear @11:03:32.11 Carousel.in :default @11:03:32.26 Carousel.home.seek 30 @11:03:32.62 Waiting for bill to answer "Turn the pump on. Push Return" @11:03:36.34 Answered Turn the pump on. Push Return"" @11:03:36.39 Vacuuming individual cartridges. @11:03:37.38 Toroid.seek :clear @11:03:37.62 Carousel.in :default @11:03:37.78 Carousel.home.seek 30 @11:03:38.40 Carousel.seek 2 @11:03:44.50 Lysis1.in :fast @11:03:44.60 Lysis1.seek :locked @11:03:47.25 Plunge.in :default @11:03:47.40 Plunge.seek :locked @11:03:47.57 Air.in :fast @11:03:47.65 Air.seek :locked @11:03:48.67 Carousel.reconfigure :locked @11:03:48.87 Powering cartridge bus @11:03:51.55 Gate.setIR 1750,5000,0,6 @11:03:51.69 Gate.setIR 0,5000,0,6 @11:03:51.80 PuckHeater.configure :default #ambient 22.0C @11:03:51.92 Gate.power :heat,:ON,limit to 5000mA @11:03:52.85 Mounted lysis Cartridge board#Q05058 @11:03:52.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:03:53.00 Vacuuming Vent Top cartridge 2 for 30 seconds @11:03:53.06 Air.in :default @11:03:53.19 Air.seek :evac @11:04:07.78 Toroid.in :hold @11:04:07.89 Toroid.seek :cartridge @11:04:09.76 Toroid.in :default @11:04:09.87 Toroid.seek :clear @11:04:11.72 Polling Can every 2:00 @11:04:12.28 PuckHeater.configure :default #ambient 22.0C @11:04:12.41 PuckHeater.seek 41.1C,1:00 @11:04:33.99 PuckHeater.hold 3 seconds,41.1C @11:04:37.14 delayUntil "11:04:40.91" @11:04:41.01 Toroid.in :hold @11:04:41.11 Toroid.seek :bypass @11:04:42.80 Toroid.in :default @11:04:42.89 Toroid.seek :clear @11:04:44.55 delay 2 seconds @11:04:46.58 Air.seek :full @11:05:01.22 Gate.power :heat,:OFF @11:05:01.25 Gate.power :cartridge,:OFF @11:05:01.40 Carousel.in :default @11:05:01.55 Carousel.home.seek 2 @11:05:02.04 Air.in :unlocking @11:05:02.20 Air.seek :clear @11:05:03.25 Lysis1.in :unlocking @11:05:03.43 Lysis1.seek :clear @11:05:03.56 Plunge.in :unlocking @11:05:03.65 Plunge.seek :clear @11:05:04.83 Carousel.seek 16 @11:05:08.05 Lysis1.in :fast @11:05:08.74 Lysis1.seek :locked @11:05:11.53 Plunge.in :default @11:05:11.69 Plunge.seek :locked @11:05:11.86 Air.in :fast @11:05:11.96 Air.seek :locked @11:05:13.00 Carousel.reconfigure :locked @11:05:13.21 Powering cartridge bus @11:05:15.88 Gate.setIR 1750,5000,0,6 @11:05:16.01 Gate.setIR 0,5000,0,6 @11:05:16.13 PuckHeater.configure :default #ambient 22.0C @11:05:16.24 Gate.power :heat,:ON,limit to 5000mA @11:05:16.95 Mounted lysis Cartridge board#Q05054 @11:05:17.03 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:05:17.08 Vacuuming Vent Top cartridge 16 for 30 seconds @11:05:17.13 Air.in :default @11:05:17.23 Air.seek :evac @11:05:31.83 Toroid.in :hold @11:05:31.92 Toroid.seek :cartridge @11:05:33.80 Toroid.in :default @11:05:33.90 Toroid.seek :clear @11:05:35.74 Polling Can every 2:00 @11:05:36.30 PuckHeater.configure :default #ambient 22.1C @11:05:36.44 PuckHeater.seek 41.1C,1:00 @11:05:58.08 PuckHeater.hold 3 seconds,41.1C @11:06:01.26 delayUntil "11:06:04.94" @11:06:05.04 Toroid.in :hold @11:06:05.14 Toroid.seek :bypass @11:06:06.82 Toroid.in :default @11:06:06.93 Toroid.seek :clear @11:06:08.59 delay 2 seconds @11:06:10.62 Air.seek :full @11:06:25.27 Gate.power :heat,:OFF @11:06:25.33 Gate.power :cartridge,:OFF @11:06:25.44 Carousel.in :default @11:06:25.60 Carousel.home.seek 16 @11:06:26.77 Air.in :unlocking @11:06:26.95 Air.seek :clear @11:06:28.02 Lysis1.in :unlocking @11:06:28.23 Lysis1.seek :clear @11:06:28.36 Plunge.in :unlocking @11:06:28.52 Plunge.seek :clear @11:06:29.67 Carousel.seek 18 @11:06:30.49 Lysis1.in :fast @11:06:30.60 Lysis1.seek :locked @11:06:33.27 Plunge.in :default @11:06:33.42 Plunge.seek :locked @11:06:33.60 Air.in :fast @11:06:33.70 Air.seek :locked @11:06:34.72 Carousel.reconfigure :locked @11:06:34.94 Powering cartridge bus @11:06:37.61 Gate.setIR 1750,5000,0,6 @11:06:37.74 Gate.setIR 0,5000,0,6 @11:06:37.85 PuckHeater.configure :default #ambient 22.1C @11:06:37.96 Gate.power :heat,:ON,limit to 5000mA @11:06:38.71 Mounted lysis Cartridge board#Q05063 @11:06:38.79 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:06:38.85 Vacuuming Vent Top cartridge 18 for 30 seconds @11:06:38.88 Air.in :default @11:06:39.01 Air.seek :evac @11:06:53.60 Toroid.in :hold @11:06:53.71 Toroid.seek :cartridge @11:06:55.58 Toroid.in :default @11:06:55.68 Toroid.seek :clear @11:06:57.54 Polling Can every 2:00 @11:06:58.09 PuckHeater.configure :default #ambient 22.2C @11:06:58.19 PuckHeater.seek 41.1C,1:00 @11:07:19.82 PuckHeater.hold 3 seconds,41.1C @11:07:22.99 delayUntil "11:07:26.73" @11:07:27.38 Toroid.in :hold @11:07:27.57 Toroid.seek :bypass @11:07:29.25 Toroid.in :default @11:07:29.35 Toroid.seek :clear @11:07:31.01 delay 2 seconds @11:07:33.04 Air.seek :full @11:07:47.68 Gate.power :heat,:OFF @11:07:47.71 Gate.power :cartridge,:OFF @11:07:47.85 Carousel.in :default @11:07:48.01 Carousel.home.seek 18 @11:07:48.49 Air.in :unlocking @11:07:48.69 Air.seek :clear @11:07:49.76 Lysis1.in :unlocking @11:07:49.95 Lysis1.seek :clear @11:07:50.06 Plunge.in :unlocking @11:07:50.20 Plunge.seek :clear @11:07:51.38 Carousel.seek 28 @11:07:53.79 Lysis1.in :fast @11:07:53.90 Lysis1.seek :locked @11:07:56.58 Plunge.in :default @11:07:56.69 Plunge.seek :locked @11:07:56.88 Air.in :fast @11:07:56.98 Air.seek :locked @11:07:58.01 Carousel.reconfigure :locked @11:07:58.21 Powering cartridge bus @11:08:00.87 Gate.setIR 1750,5000,0,6 @11:08:01.00 Gate.setIR 0,5000,0,6 @11:08:01.11 PuckHeater.configure :default #ambient 22.2C @11:08:01.24 Gate.power :heat,:ON,limit to 5000mA @11:08:01.94 Mounted lysis Cartridge board#Q05045 @11:08:02.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:08:02.07 Vacuuming Vent Top cartridge 28 for 30 seconds @11:08:02.12 Air.in :default @11:08:02.24 Air.seek :evac @11:08:16.81 Toroid.in :hold @11:08:16.92 Toroid.seek :cartridge @11:08:18.79 Toroid.in :default @11:08:18.89 Toroid.seek :clear @11:08:20.74 Polling Can every 2:00 @11:08:21.30 PuckHeater.configure :default #ambient 22.3C @11:08:21.40 PuckHeater.seek 41.1C,1:00 @11:08:42.55 PuckHeater.hold 3 seconds,41.1C @11:08:45.78 delayUntil "11:08:49.94" @11:08:50.04 Toroid.in :hold @11:08:50.15 Toroid.seek :bypass @11:08:51.84 Toroid.in :default @11:08:51.96 Toroid.seek :clear @11:08:53.60 delay 2 seconds @11:08:55.63 Air.seek :full @11:09:10.28 Gate.power :heat,:OFF @11:09:10.31 Gate.power :cartridge,:OFF @11:09:10.48 Carousel.in :default @11:09:10.64 Carousel.home.seek 28 @11:09:11.10 Air.in :unlocking @11:09:11.27 Air.seek :clear @11:09:12.33 Lysis1.in :unlocking @11:09:12.54 Lysis1.seek :clear @11:09:12.61 Plunge.in :unlocking @11:09:12.80 Plunge.seek :clear @11:09:14.03 Carousel.seek 30 @11:09:14.83 Lysis1.in :fast @11:09:14.93 Lysis1.seek :locked @11:09:17.60 Plunge.in :default @11:09:17.68 Plunge.seek :locked @11:09:17.89 Air.in :fast @11:09:18.00 Air.seek :locked @11:09:19.01 Carousel.reconfigure :locked @11:09:19.21 Powering cartridge bus @11:09:21.88 Gate.setIR 1750,5000,0,6 @11:09:22.02 Gate.setIR 0,5000,0,6 @11:09:22.12 PuckHeater.configure :default #ambient 22.3C @11:09:22.24 Gate.power :heat,:ON,limit to 5000mA @11:09:22.96 Mounted lysis Cartridge board#Q05064 @11:09:23.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:09:23.10 Vacuuming Vent Top cartridge 30 for 30 seconds @11:09:23.15 Air.in :default @11:09:23.25 Air.seek :evac @11:09:38.44 Toroid.in :hold @11:09:38.65 Toroid.seek :cartridge @11:09:40.53 Toroid.in :default @11:09:40.64 Toroid.seek :clear @11:09:42.50 Polling Can every 2:00 @11:09:43.06 PuckHeater.configure :default #ambient 22.3C @11:09:43.16 PuckHeater.seek 41C,1:00 @11:10:04.80 PuckHeater.hold 3 seconds,41C @11:10:07.94 delayUntil "11:10:11.67" @11:10:11.78 Toroid.in :hold @11:10:11.88 Toroid.seek :bypass @11:10:13.56 Toroid.in :default @11:10:13.66 Toroid.seek :clear @11:10:15.31 delay 2 seconds @11:10:17.34 Air.seek :full @11:10:31.85 Gate.power :heat,:OFF @11:10:31.88 Gate.power :cartridge,:OFF @11:10:32.02 Carousel.in :default @11:10:32.17 Carousel.home.seek 30 @11:10:32.64 Air.in :unlocking @11:10:32.83 Air.seek :clear @11:10:33.89 Lysis1.in :unlocking @11:10:34.09 Lysis1.seek :clear @11:10:34.14 Plunge.in :unlocking @11:10:34.31 Plunge.seek :clear @11:10:35.53 Cartridges under vacuum. Individual Leak Test begins @11:10:35.56 Waiting for bill to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @11:10:58.88 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @11:10:58.91 delay 10:00 @11:20:58.97 Waiting for bill to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @11:24:12.89 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @11:24:12.92 move through each cartridge position & record end vacuum @11:24:12.96 Vacuuming cartridge 2 @11:24:13.33 Carousel.seek 2 @11:24:19.40 Lysis1.in :fast @11:24:19.51 Lysis1.seek :locked @11:24:22.83 Plunge.in :default @11:24:22.92 Plunge.seek :locked @11:24:23.15 Air.in :fast @11:24:23.23 Air.seek :locked @11:24:24.25 Carousel.reconfigure :locked @11:24:24.49 Powering cartridge bus @11:24:27.16 Gate.setIR 1750,5000,0,6 @11:24:27.29 Gate.setIR 0,5000,0,6 @11:24:27.40 PuckHeater.configure :default #ambient 22.3C @11:24:27.52 Gate.power :heat,:ON,limit to 5000mA @11:24:28.22 Mounted lysis Cartridge board#Q05058 @11:24:28.31 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:24:28.49 Toroid.in :hold @11:24:28.60 Toroid.seek :cartridge @11:24:30.48 Toroid.in :default @11:24:30.59 Toroid.seek :clear @11:24:32.45 Waiting for bill to answer "record Vacuum" @11:24:48.32 Answered record Vacuum"" @11:24:48.50 Toroid.in :hold @11:24:48.60 Toroid.seek :bypass @11:24:50.28 Toroid.in :default @11:24:50.42 Toroid.seek :clear @11:24:52.08 Waiting for bill to answer "recharge if needed" @11:26:02.97 Answered recharge if needed"" @11:26:02.98 Vacuuming cartridge 16 @11:26:03.21 Gate.power :heat,:OFF @11:26:03.24 Gate.power :cartridge,:OFF @11:26:03.39 Carousel.in :default @11:26:03.55 Carousel.home.seek 2 @11:26:04.01 Air.in :unlocking @11:26:04.17 Air.seek :clear @11:26:05.23 Lysis1.in :unlocking @11:26:05.45 Lysis1.seek :clear @11:26:05.50 Plunge.in :unlocking @11:26:05.70 Plunge.seek :clear @11:26:06.95 Carousel.seek 16 @11:26:10.18 Lysis1.in :fast @11:26:10.28 Lysis1.seek :locked @11:26:12.99 Plunge.in :default @11:26:13.14 Plunge.seek :locked @11:26:13.28 Air.in :fast @11:26:13.39 Air.seek :locked @11:26:14.41 Carousel.reconfigure :locked @11:26:14.62 Powering cartridge bus @11:26:17.40 Gate.setIR 1750,5000,0,6 @11:26:17.55 Gate.setIR 0,5000,0,6 @11:26:17.65 PuckHeater.configure :default #ambient 22.3C @11:26:17.76 Gate.power :heat,:ON,limit to 5000mA @11:26:18.49 Mounted lysis Cartridge board#Q05054 @11:26:18.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:26:18.73 Toroid.in :hold @11:26:18.84 Toroid.seek :cartridge @11:26:20.71 Toroid.in :default @11:26:20.83 Toroid.seek :clear @11:26:22.66 Waiting for bill to answer "record Vacuum" @11:26:30.31 Answered record Vacuum"" @11:26:30.50 Toroid.in :hold @11:26:30.60 Toroid.seek :bypass @11:26:32.28 Toroid.in :default @11:26:32.41 Toroid.seek :clear @11:26:34.07 Waiting for bill to answer "recharge if needed" @11:26:42.00 Answered recharge if needed"" @11:26:42.02 Vacuuming cartridge 18 @11:26:42.25 Gate.power :heat,:OFF @11:26:42.27 Gate.power :cartridge,:OFF @11:26:42.49 Carousel.in :default @11:26:42.65 Carousel.home.seek 16 @11:26:43.13 Air.in :unlocking @11:26:43.30 Air.seek :clear @11:26:44.40 Lysis1.in :unlocking @11:26:44.61 Lysis1.seek :clear @11:26:44.69 Plunge.in :unlocking @11:26:44.85 Plunge.seek :clear @11:26:46.10 Carousel.seek 18 @11:26:46.89 Lysis1.in :fast @11:26:46.99 Lysis1.seek :locked @11:26:49.67 Plunge.in :default @11:26:49.82 Plunge.seek :locked @11:26:49.96 Air.in :fast @11:26:50.08 Air.seek :locked @11:26:51.09 Carousel.reconfigure :locked @11:26:51.30 Powering cartridge bus @11:26:53.97 Gate.setIR 1750,5000,0,6 @11:26:54.10 Gate.setIR 0,5000,0,6 @11:26:54.21 PuckHeater.configure :default #ambient 22.3C @11:26:54.33 Gate.power :heat,:ON,limit to 5000mA @11:26:55.08 Mounted lysis Cartridge board#Q05063 @11:26:55.16 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:26:55.32 Toroid.in :hold @11:26:55.42 Toroid.seek :cartridge @11:26:57.95 Toroid.in :default @11:26:58.08 Toroid.seek :clear @11:26:59.93 Waiting for bill to answer "record Vacuum" @11:27:06.56 Answered record Vacuum"" @11:27:06.71 Toroid.in :hold @11:27:06.81 Toroid.seek :bypass @11:27:08.49 Toroid.in :default @11:27:08.60 Toroid.seek :clear @11:27:10.26 Waiting for bill to answer "recharge if needed" @11:27:19.36 Answered recharge if needed"" @11:27:19.38 Vacuuming cartridge 28 @11:27:19.61 Gate.power :heat,:OFF @11:27:19.64 Gate.power :cartridge,:OFF @11:27:19.78 Carousel.in :default @11:27:19.93 Carousel.home.seek 18 @11:27:20.44 Air.in :unlocking @11:27:20.61 Air.seek :clear @11:27:21.66 Lysis1.in :unlocking @11:27:21.86 Lysis1.seek :clear @11:27:21.99 Plunge.in :unlocking @11:27:22.12 Plunge.seek :clear @11:27:23.31 Carousel.seek 28 @11:27:25.71 Lysis1.in :fast @11:27:25.82 Lysis1.seek :locked @11:27:28.50 Plunge.in :default @11:27:28.59 Plunge.seek :locked @11:27:28.79 Air.in :fast @11:27:28.88 Air.seek :locked @11:27:29.88 Carousel.reconfigure :locked @11:27:30.13 Powering cartridge bus @11:27:32.81 Gate.setIR 1750,5000,0,6 @11:27:32.95 Gate.setIR 0,5000,0,6 @11:27:33.05 PuckHeater.configure :default #ambient 22.3C @11:27:33.17 Gate.power :heat,:ON,limit to 5000mA @11:27:33.88 Mounted lysis Cartridge board#Q05045 @11:27:33.96 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:27:34.12 Toroid.in :hold @11:27:34.23 Toroid.seek :cartridge @11:27:36.11 Toroid.in :default @11:27:36.21 Toroid.seek :clear @11:27:38.07 Waiting for bill to answer "record Vacuum" @11:27:41.94 Answered record Vacuum"" @11:27:42.10 Toroid.in :hold @11:27:42.20 Toroid.seek :bypass @11:27:43.88 Toroid.in :default @11:27:43.98 Toroid.seek :clear @11:27:46.29 Waiting for bill to answer "recharge if needed" @11:27:54.48 Answered recharge if needed"" @11:27:54.49 Vacuuming cartridge 30 @11:27:54.74 Gate.power :heat,:OFF @11:27:54.79 Gate.power :cartridge,:OFF @11:27:54.91 Carousel.in :default @11:27:55.07 Carousel.home.seek 28 @11:27:55.54 Air.in :unlocking @11:27:55.69 Air.seek :clear @11:27:56.73 Lysis1.in :unlocking @11:27:56.91 Lysis1.seek :clear @11:27:57.00 Plunge.in :unlocking @11:27:57.17 Plunge.seek :clear @11:27:58.42 Carousel.seek 30 @11:27:59.22 Lysis1.in :fast @11:27:59.33 Lysis1.seek :locked @11:28:02.00 Plunge.in :default @11:28:02.15 Plunge.seek :locked @11:28:02.33 Air.in :fast @11:28:02.46 Air.seek :locked @11:28:03.46 Carousel.reconfigure :locked @11:28:03.67 Powering cartridge bus @11:28:06.33 Gate.setIR 1750,5000,0,6 @11:28:06.49 Gate.setIR 0,5000,0,6 @11:28:06.60 PuckHeater.configure :default #ambient 22.3C @11:28:06.71 Gate.power :heat,:ON,limit to 5000mA @11:28:07.42 Mounted lysis Cartridge board#Q05064 @11:28:07.50 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:28:07.67 Toroid.in :hold @11:28:07.76 Toroid.seek :cartridge @11:28:09.63 Toroid.in :default @11:28:09.74 Toroid.seek :clear @11:28:11.60 Waiting for bill to answer "record Vacuum" @11:28:17.73 Answered record Vacuum"" @11:28:17.87 Toroid.in :hold @11:28:17.97 Toroid.seek :bypass @11:28:19.65 Toroid.in :default @11:28:19.76 Toroid.seek :clear @11:28:21.42 Waiting for bill to answer "recharge if needed" @11:28:32.38 Answered recharge if needed"" @11:28:32.46 Gate.power :heat,:OFF @11:28:32.48 Gate.power :cartridge,:OFF @11:28:32.60 Carousel.in :default @11:28:32.79 Carousel.home.seek 30 @11:28:33.24 Air.in :unlocking @11:28:33.36 Air.seek :clear @11:28:34.50 Lysis1.in :unlocking @11:28:34.70 Lysis1.seek :clear @11:28:34.78 Plunge.in :unlocking @11:28:34.96 Plunge.seek :clear @11:28:36.21 Waiting for bill to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @11:28:39.83 UserAbort in bill -- User Abort!! @11:29:20.92 -> SC.to 2 @11:29:20.97 Carousel.seek 2 Storage Carousel (SC) at 2 @11:31:37.84 -> SC.to 16 @11:31:37.90 Carousel.seek 16 Storage Carousel (SC) at 16 @11:33:30.99 -> slot {[30,28,18,16,2] => { Type: :lyseHold_phyto, State: :dry}} @11:33:52.80 -> slot [30, 28, 18] =>nil {[30,28,18] => nil} @11:33:55.07 -> slot {[16,2] => { Type: :lyseHold_phyto, State: :dry}} @11:34:09.35 -> vacToroid @11:34:09.41 Vacuuming protocol for dry cartridges @11:34:10.26 Toroid.seek :clear @11:34:10.52 Carousel.in :default @11:34:10.68 Carousel.home.seek 16 @11:34:11.00 Waiting for bill to answer "Disconnect the intake line. push return" @11:34:17.05 Answered Disconnect the intake line. push return"" @11:34:17.86 Toroid.seek :clear @11:34:18.09 Carousel.in :default @11:34:18.24 Carousel.home.seek 16 @11:34:18.55 SamplePump.setPosition! 0ml @11:34:18.64 Intake.open @11:34:19.37 SamplePump.seek 50ml @11:35:20.99 Exhaust.close @11:35:21.69 SamplePump.seek 75ml @11:35:52.82 Intake.close @11:35:53.58 Bypass.close @11:35:54.32 Waiting for bill to answer "Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return" @11:37:48.30 Answered Hook up vacuum pump to the Exhaust Port. Open valve on Flask. Push Return"" @11:37:48.32 Exhaust.open @11:37:49.06 Bypass.open @11:37:49.76 Waiting for bill to answer "Turn on vacuum to remove residual fluid in sample path. Press Return" @11:37:54.70 Answered Turn on vacuum to remove residual fluid in sample path. Press Return"" @11:37:54.73 delay 30 seconds @11:38:24.79 Clearing fluid from cartridge-side of Toroid @11:38:25.11 Carousel.seek 2 @11:38:28.20 Toroid.in :hold @11:38:28.31 Toroid.seek :cartridge @11:38:30.21 Toroid.in :default @11:38:30.33 Toroid.seek :clear @11:38:32.21 delay 2 seconds @11:38:34.32 Toroid.in :hold @11:38:35.10 Toroid.seek :bypass @11:38:36.76 Toroid.in :default @11:38:36.87 Toroid.seek :clear @11:38:38.80 Carousel.seek 16 @11:38:41.90 Toroid.in :hold @11:38:42.01 Toroid.seek :cartridge @11:38:43.89 Toroid.in :default @11:38:43.99 Toroid.seek :clear @11:38:45.84 delay 2 seconds @11:38:47.96 Toroid.in :hold @11:38:48.07 Toroid.seek :bypass @11:38:49.75 Toroid.in :default @11:38:49.85 Toroid.seek :clear @11:38:51.50 Waiting for bill to answer "Turn the pump off & load the cartridges. Press Return when loaded." @11:38:54.65 Answered Turn the pump off & load the cartridges. Press Return when loaded."" @11:38:55.50 Toroid.seek :clear @11:38:55.72 Carousel.in :default @11:38:55.87 Carousel.home.seek 16 @11:38:56.17 Waiting for bill to answer "Turn the pump on. Push Return" @11:38:59.31 Answered Turn the pump on. Push Return"" @11:38:59.32 Vacuuming individual cartridges. @11:39:00.25 Toroid.seek :clear @11:39:00.50 Carousel.in :default @11:39:00.66 Carousel.home.seek 16 @11:39:01.27 Carousel.seek 2 @11:39:04.53 Lysis1.in :fast @11:39:04.63 Lysis1.seek :locked @11:39:07.30 Plunge.in :default @11:39:07.45 Plunge.seek :locked @11:39:07.61 Air.in :fast @11:39:07.72 Air.seek :locked @11:39:08.74 Carousel.reconfigure :locked @11:39:08.94 Powering cartridge bus @11:39:11.72 Gate.setIR 1750,5000,0,6 @11:39:11.88 Gate.setIR 0,5000,0,6 @11:39:11.99 PuckHeater.configure :default #ambient 22.3C @11:39:12.11 Gate.power :heat,:ON,limit to 5000mA @11:39:12.85 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @11:39:12.94 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:39:13.01 Vacuuming Vent Top cartridge 2 for 30 seconds @11:39:13.04 Air.in :default @11:39:13.16 Air.seek :evac @11:39:27.75 Toroid.in :hold @11:39:27.86 Toroid.seek :cartridge @11:39:29.73 Toroid.in :default @11:39:29.85 Toroid.seek :clear @11:39:31.69 Polling Can every 2:00 @11:39:32.25 PuckHeater.configure :default #ambient 22.1C @11:39:32.39 PuckHeater.seek 41.1C,1:00 @11:39:53.99 PuckHeater.hold 3 seconds,41.1C @11:39:57.14 delayUntil "11:40:00.88" @11:40:00.99 Toroid.in :hold @11:40:01.09 Toroid.seek :bypass @11:40:02.78 Toroid.in :default @11:40:02.88 Toroid.seek :clear @11:40:04.54 delay 2 seconds @11:40:06.57 Air.seek :full @11:40:21.21 Gate.power :heat,:OFF @11:40:21.24 Gate.power :cartridge,:OFF @11:40:21.39 Carousel.in :default @11:40:21.54 Carousel.home.seek 2 @11:40:21.99 Air.in :unlocking @11:40:22.12 Air.seek :clear @11:40:23.19 Lysis1.in :unlocking @11:40:23.36 Lysis1.seek :clear @11:40:23.50 Plunge.in :unlocking @11:40:23.60 Plunge.seek :clear @11:40:24.76 Carousel.seek 16 @11:40:28.55 Lysis1.in :fast @11:40:28.74 Lysis1.seek :locked @11:40:31.42 Plunge.in :default @11:40:31.50 Plunge.seek :locked @11:40:31.75 Air.in :fast @11:40:31.84 Air.seek :locked @11:40:32.86 Carousel.reconfigure :locked @11:40:33.07 Powering cartridge bus @11:40:35.74 Gate.setIR 1750,5000,0,6 @11:40:35.88 Gate.setIR 0,5000,0,6 @11:40:35.98 PuckHeater.configure :default #ambient 22.1C @11:40:36.09 Gate.power :heat,:ON,limit to 5000mA @11:40:36.79 Mounted lysis Cartridge board#Q05048 @11:40:36.87 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:40:36.97 Vacuuming Vent Top cartridge 16 for 30 seconds @11:40:37.00 Air.in :default @11:40:37.11 Air.seek :evac @11:40:51.72 Toroid.in :hold @11:40:51.83 Toroid.seek :cartridge @11:40:53.71 Toroid.in :default @11:40:53.81 Toroid.seek :clear @11:40:55.66 Polling Can every 2:00 @11:40:56.22 PuckHeater.configure :default #ambient 22.3C @11:40:56.33 PuckHeater.seek 41.1C,1:00 @11:41:17.97 PuckHeater.hold 3 seconds,41.1C @11:41:21.12 delayUntil "11:41:24.85" @11:41:24.95 Toroid.in :hold @11:41:25.06 Toroid.seek :bypass @11:41:26.74 Toroid.in :default @11:41:26.85 Toroid.seek :clear @11:41:28.50 delay 2 seconds @11:41:30.53 Air.seek :full @11:41:45.06 Gate.power :heat,:OFF @11:41:45.11 Gate.power :cartridge,:OFF @11:41:45.22 Carousel.in :default @11:41:45.38 Carousel.home.seek 16 @11:41:46.54 Air.in :unlocking @11:41:46.73 Air.seek :clear @11:41:47.79 Lysis1.in :unlocking @11:41:47.96 Lysis1.seek :clear @11:41:48.13 Plunge.in :unlocking @11:41:48.25 Plunge.seek :clear @11:41:49.40 Cartridges under vacuum. Individual Leak Test begins @11:41:49.45 Waiting for bill to answer "Close valve on vacuum flask & turn off vacuum. Record Start Vacuum." @11:42:00.61 Answered Close valve on vacuum flask & turn off vacuum. Record Start Vacuum."" @11:42:00.69 delay 10:00 @11:52:00.75 Waiting for bill to answer "if Vacuum dropped in sample bypass then recharge before proceeding" @11:52:25.67 Answered if Vacuum dropped in sample bypass then recharge before proceeding"" @11:52:25.69 move through each cartridge position & record end vacuum @11:52:25.74 Vacuuming cartridge 2 @11:52:26.10 Carousel.seek 2 @11:52:29.34 Lysis1.in :fast @11:52:29.48 Lysis1.seek :locked @11:52:32.14 Plunge.in :default @11:52:32.29 Plunge.seek :locked @11:52:32.47 Air.in :fast @11:52:32.57 Air.seek :locked @11:52:33.59 Carousel.reconfigure :locked @11:52:33.79 Powering cartridge bus @11:52:36.49 Gate.setIR 1750,5000,0,6 @11:52:36.62 Gate.setIR 0,5000,0,6 @11:52:36.73 PuckHeater.configure :default #ambient 22.3C @11:52:36.84 Gate.power :heat,:ON,limit to 5000mA @11:52:37.58 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @11:52:37.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:52:37.84 Toroid.in :hold @11:52:37.95 Toroid.seek :cartridge @11:52:39.82 Toroid.in :default @11:52:39.93 Toroid.seek :clear @11:52:41.78 Waiting for bill to answer "record Vacuum" @11:52:45.02 Answered record Vacuum"" @11:52:45.17 Toroid.in :hold @11:52:45.27 Toroid.seek :bypass @11:52:46.96 Toroid.in :default @11:52:47.05 Toroid.seek :clear @11:52:48.71 Waiting for bill to answer "recharge if needed" @11:55:06.33 Answered recharge if needed"" @11:55:06.35 Vacuuming cartridge 16 @11:55:06.61 Gate.power :heat,:OFF @11:55:06.64 Gate.power :cartridge,:OFF @11:55:06.79 Carousel.in :default @11:55:06.95 Carousel.home.seek 2 @11:55:07.39 Air.in :unlocking @11:55:08.22 Air.seek :clear @11:55:09.29 Lysis1.in :unlocking @11:55:09.45 Lysis1.seek :clear @11:55:09.63 Plunge.in :unlocking @11:55:09.73 Plunge.seek :clear @11:55:10.89 Carousel.seek 16 @11:55:14.11 Lysis1.in :fast @11:55:14.21 Lysis1.seek :locked @11:55:16.87 Plunge.in :default @11:55:17.02 Plunge.seek :locked @11:55:17.19 Air.in :fast @11:55:17.29 Air.seek :locked @11:55:18.30 Carousel.reconfigure :locked @11:55:18.54 Powering cartridge bus @11:55:21.21 Gate.setIR 1750,5000,0,6 @11:55:21.35 Gate.setIR 0,5000,0,6 @11:55:21.45 PuckHeater.configure :default #ambient 22.3C @11:55:21.59 Gate.power :heat,:ON,limit to 5000mA @11:55:22.29 Mounted lysis Cartridge board#Q05048 @11:55:22.37 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:55:22.58 Toroid.in :hold @11:55:22.68 Toroid.seek :cartridge @11:55:24.57 Toroid.in :default @11:55:24.66 Toroid.seek :clear @11:55:26.51 Waiting for bill to answer "record Vacuum" @11:55:38.39 Answered record Vacuum"" @11:55:38.55 Toroid.in :hold @11:55:38.64 Toroid.seek :bypass @11:55:40.32 Toroid.in :default @11:55:40.46 Toroid.seek :clear @11:55:42.13 Waiting for bill to answer "recharge if needed" @11:55:52.10 Answered recharge if needed"" @11:55:52.16 Gate.power :heat,:OFF @11:55:52.19 Gate.power :cartridge,:OFF @11:55:52.32 Carousel.in :default @11:55:52.52 Carousel.home.seek 16 @11:55:52.98 Air.in :unlocking @11:55:53.12 Air.seek :clear @11:55:54.16 Lysis1.in :unlocking @11:55:54.32 Lysis1.seek :clear @11:55:54.51 Plunge.in :unlocking @11:55:54.61 Plunge.seek :clear @11:55:55.77 Waiting for bill to answer "Record End Vacuum. If all cartridges passed, run refillSampleLines, else repeat protocol." @11:56:02.21 UserAbort in bill -- User Abort!! @11:58:27.23 -> ESP.shutdown @11:58:27.37 Exhaust.close @11:58:27.44 Bypass.close @11:58:29.05 Gate.power :analytic1,:OFF @11:58:29.08 Gate.power :motor,:OFF @11:58:29.15 Gate.power :core,:OFF @11:58:29.19 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @13:48:26.68PDT01-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:48:28.44 >BREAK< ESP3 Gateway v1.43 for core bus @13:48:28.47 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:48:32.11 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @13:48:32.79 Sea pressure (Abs) tare is 6% of full scale @13:48:46.20 Polling Can every 2:00 @13:48:46.26 Waiting for espclients... @13:48:53.58 Session Begun @13:48:56.89 -> slot {[16,2] => { Type: :lyseHold_phyto, State: :dry}} @13:49:07.24 -> slot [60..1] =>nil {60..1 => nil} @13:51:26.44 -> slot [58, 56, 51, 49, 45, 43, 41, 39, 37, 35, 33, 31, 29, 27, 25, 23, 21, 19, 17, 15, 13, 11, 9, 7, 5, 3] =>[:dry, :archiveHiBiomass_bac] {[58,56,51,49,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry}} @13:52:26.62 -> slot 47 =>[:dry, :archiveHiBiomass_bac] {47 => { Type: :archiveHiBiomass_bac, State: :dry}} @13:52:30.02 -> slot {[58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry}} @13:54:14.51 -> slot [60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 14, 12, 10, 8, 6, 4, 2, 1] =>[:dry, :microSPR_phyto] {[60,59,57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}} @13:55:17.83 -> slot 16 =>[:dry, :microSPR_phyto] {16 => { Type: :microSPR_phyto, State: :dry}} @13:55:22.88 -> slot {[60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry}} @13:57:22.68 -> slot [55..52] =>[:microSPRphyto, :standard, {lysed:1100, lysed2:1100}] @13:57:22.76 Cartridge::TypeUnknown in bill -- :microSPRphyto is not aRNAl_bac, aRNAl_phyto, aRNAl_zoo, archiveHiBiomass_bac, archiveNull_bac, archive_bac, archive_phyto, archive_zoo, blank_bac, blank_phyto, daSPR_bac, daSPR_phyto, daSPR_phyto9, daSPR_zoo, lyseHold_bac, lyseHold_phyto, lyseHold_zoo, lyseNull_bac, microSPR_bac, microSPR_phyto, microSPR_zoo or unknown @13:57:44.03 -> slot [55..52] =>[:microSPR_phyto, :standard, {lysed:1100, lysed2:1100}] {55..52 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @13:57:55.30 -> slot {[60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 55..52 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @14:00:02.40 -> Intake.open @14:00:02.42 Intake.open @14:00:02.46 BusOff in bill -- Intake is not powered! @14:00:08.17 -> intake.open @14:00:08.22 NoMethodError in bill -- private method `open' called for :intake:Symbol @14:00:16.15 -> ESP.ready! @14:00:16.19 Gate.power :sampler,:OFF,limit to 3500mA @14:00:16.21 Gate.power :skimmer,:OFF,limit to 3500mA @14:00:16.25 Gate.power :main,:ON,limit to 5000mA @14:00:16.28 Gate.power :analytic1,:ON,limit to 4000mA @14:00:16.49 Powering core bus @14:00:18.72 SPR Module 1, FW Rev. B, FPGA Rev. C @14:00:19.27 Plunge.configure :default @14:00:19.37 SamplePump.configure :default @14:00:19.47 Toroid.configure :default @14:00:19.57 Carousel.configure :default @14:00:19.67 Abs.configure :default @14:00:19.77 PhaseSeparator.configure :default @14:00:19.88 Archive.configure :default @14:00:19.98 Air.configure :default @14:00:20.06 Lysis1.configure :default @14:00:20.13 Lysis2.configure :default @14:00:20.24 PinPort.configure :default @14:00:20.31 All elf controllers running firmware version 4.92 @14:00:20.34 Gate.power :motor,:ON,limit to 5000mA @14:00:20.67 Plunge.home.jog 0 @14:00:21.42 Lysis2.home.jog 0 @14:00:21.48 Air.home.jog 0 @14:00:21.53 Carousel.home.jog 0 @14:00:21.58 Lysis1.home.jog 0 @14:00:21.61 Archive.home.jog 0 @14:00:21.64 PinPort.home.jog 0 @14:00:21.71 Toroid.home.jog 0 @14:00:22.50 Plunge.in :homing @14:00:22.53 Lysis2.in :default @14:00:22.61 Archive.in :default @14:00:22.77 Plunge.jog 165 @14:00:22.87 Archive.home.jog 0 @14:00:22.92 Lysis2.home.jog 0 @14:00:23.27 PinPort.in :homing @14:00:23.36 Air.in :homing @14:00:23.40 Toroid.in :homing @14:00:23.45 Lysis2.in :homing @14:00:23.49 Archive.in :homing @14:00:23.53 Lysis1.in :homing @14:00:24.28 PinPort.jog -106 @14:00:24.36 Air.jog 1386 @14:00:24.42 Toroid.jog 617 @14:00:24.46 Lysis2.jog 11700 @14:00:24.51 Lysis1.jog 1393 @14:00:24.59 Archive.jog 11700 @14:00:25.04 PinPort.setRawPosition! 0 @14:00:25.07 Plunge.setRawPosition! 50 @14:00:25.20 PinPort.seek :home @14:00:25.24 Plunge.seek :home @14:00:25.46 PinPort.in :default @14:00:25.61 Plunge.in :default @14:00:25.90 Air.setRawPosition! 50 @14:00:25.98 Air.seek :home @14:00:26.10 Lysis1.setRawPosition! 50 @14:00:26.18 Lysis1.seek :home @14:00:26.32 Air.in :default @14:00:26.50 Lysis1.in :default @14:00:26.84 Toroid.setRawPosition! 0 @14:00:26.91 Toroid.seek :home @14:00:27.07 Toroid.in :default @14:00:30.74 Lysis2.setRawPosition! 50 @14:00:30.83 Archive.setRawPosition! 50 @14:00:30.87 Lysis2.seek :home @14:00:31.03 Archive.seek :home @14:00:31.24 Lysis2.in :default @14:00:31.35 Archive.in :default @14:00:31.70 Toroid.seek :clear @14:00:33.39 Carousel.in :default @14:00:33.54 Carousel.home.seek 13 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 13 Abs = Absolute Pressure (Leak) Sensor @14psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:00:39.26 -> Intake.open @14:00:39.28 Intake.open Intake is opened @14:00:56.28 -> Intake.closed @14:00:56.31 NoMethodError in bill -- undefined method `closed' for Intake:ExternalValve @14:01:02.77 -> Intake.close @14:01:02.79 Intake.close Intake is closed @14:01:09.23 -> Exhaust.open @14:01:09.26 Exhaust.open Exhaust is opened @14:01:16.87 -> Exhaust.xlose @14:01:16.90 NoMethodError in bill -- undefined method `xlose' for Exhaust:ExternalValve @14:01:22.92 -> Exhaust.close @14:01:22.94 Exhaust.close Exhaust is closed @14:01:26.65 -> PB.oprn @14:01:26.69 NoMethodError in bill -- undefined method `oprn' for Bypass:ExternalValve @14:01:30.45 -> PB.open @14:01:30.47 Bypass.open Bypass (PB) is opened @14:01:36.85 -> PB.close @14:01:36.89 Bypass.close Bypass (PB) is closed @14:01:42.99 -> PB.open @14:01:43.03 Bypass.open Bypass (PB) is opened @14:01:50.10 -> PB.close @14:01:50.12 Bypass.close Bypass (PB) is closed @14:02:04.65 -> SC.to 60 @14:02:04.70 Carousel.seek 60 Storage Carousel (SC) at 60 @14:02:19.89 -> SC.to 36 @14:02:19.94 Carousel.seek 36 Storage Carousel (SC) at 36 @14:02:34.02 -> SC.to 1 @14:02:34.05 Carousel.seek 1 Storage Carousel (SC) at 1 @14:05:03.22 -> SC.to 60 @14:05:03.25 Carousel.seek 60 Storage Carousel (SC) at 60 @14:05:28.05 -> SC.to 58 @14:05:28.09 Carousel.seek 58 Storage Carousel (SC) at 58 @14:05:34.39 -> Cartridge.lock @14:05:34.57 Lysis1.in :fast @14:05:34.66 Lysis1.seek :locked @14:05:37.79 Plunge.seek :locked @14:05:37.99 Air.in :fast @14:05:38.06 Air.seek :locked @14:05:39.06 Carousel.reconfigure :locked @14:05:39.24 Powering cartridge bus @14:05:41.91 Gate.setIR 1750,5000,0,6 @14:05:42.05 Gate.setIR 0,5000,0,6 @14:05:42.15 Gate.power :cartridge,:OFF @14:05:42.72 Mounted archive Cartridge :archiveHiBiomass_bac @14:05:56.86 -> Cartridge.unlock @14:05:56.97 Carousel.in :default @14:05:57.15 Carousel.home.seek 58 @14:05:57.59 Air.in :unlocking @14:05:57.75 Air.seek :clear @14:05:58.80 Lysis1.in :unlocking @14:05:58.93 Lysis1.seek :clear @14:05:59.09 Plunge.in :unlocking @14:05:59.19 Plunge.seek :clear @14:06:11.60 -> Cartridge.lock @14:06:11.78 Lysis1.in :fast @14:06:11.87 Lysis1.seek :locked @14:06:14.52 Plunge.in :default @14:06:14.64 Plunge.seek :locked @14:06:14.80 Air.in :fast @14:06:14.87 Air.seek :locked @14:06:15.87 Carousel.reconfigure :locked @14:06:16.06 Powering cartridge bus @14:06:18.72 Gate.setIR 1750,5000,0,6 @14:06:18.85 Gate.setIR 0,5000,0,6 @14:06:18.95 Gate.power :cartridge,:OFF @14:06:19.56 Mounted archive Cartridge :archiveHiBiomass_bac @14:06:27.20 -> Cartridge.unlock @14:06:27.32 Carousel.in :default @14:06:27.47 Carousel.home.seek 58 @14:06:27.90 Air.in :unlocking @14:06:28.06 Air.seek :clear @14:06:29.12 Lysis1.in :unlocking @14:06:29.28 Lysis1.seek :clear @14:06:29.37 Plunge.in :unlocking @14:06:29.50 Plunge.seek :clear @14:12:39.26 -> to 1; to 15; to 34; to 48; to 60 @14:12:39.57 Carousel.seek 1 @14:12:51.53 Lysis1.in :fast @14:12:51.60 Lysis1.seek :locked @14:12:54.24 Plunge.in :default @14:12:54.37 Plunge.seek :locked @14:12:54.90 Air.in :fast @14:12:55.12 Air.seek :locked @14:12:56.11 Carousel.reconfigure :locked @14:12:56.29 Powering cartridge bus @14:12:58.96 Gate.setIR 1750,5000,0,6 @14:12:59.11 Gate.setIR 0,5000,0,6 @14:12:59.20 PuckHeater.configure :default @14:12:59.29 Gate.power :heat,:ON,limit to 5000mA @14:13:00.04 Mounted lysis Cartridge board#Q05059 @14:13:00.08 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:13:00.31 Gate.power :heat,:OFF @14:13:00.33 Gate.power :cartridge,:OFF @14:13:00.45 Carousel.in :default @14:13:00.60 Carousel.home.seek 1 @14:13:01.08 Air.in :unlocking @14:13:01.24 Air.seek :clear @14:13:02.28 Lysis1.in :unlocking @14:13:02.42 Lysis1.seek :clear @14:13:02.56 Plunge.in :unlocking @14:13:02.64 Plunge.seek :clear @14:13:03.80 Carousel.seek 15 @14:13:07.03 Lysis1.in :fast @14:13:07.11 Lysis1.seek :locked @14:13:09.76 Plunge.in :default @14:13:09.88 Plunge.seek :locked @14:13:10.03 Air.in :fast @14:13:10.12 Air.seek :locked @14:13:11.12 Carousel.reconfigure :locked @14:13:11.30 Powering cartridge bus @14:13:13.97 Gate.setIR 1750,5000,0,6 @14:13:14.10 Gate.setIR 0,5000,0,6 @14:13:14.20 Gate.power :cartridge,:OFF @14:13:14.76 Mounted archive Cartridge @14:13:15.08 Carousel.in :default @14:13:15.22 Carousel.home.seek 15 @14:13:15.68 Air.in :unlocking @14:13:15.82 Air.seek :clear @14:13:16.84 Lysis1.in :unlocking @14:13:17.00 Lysis1.seek :clear @14:13:17.14 Plunge.in :unlocking @14:13:17.23 Plunge.seek :clear @14:13:18.39 Carousel.seek 34 @14:13:22.62 Lysis1.in :fast @14:13:22.70 Lysis1.seek :locked @14:13:25.87 Plunge.in :default @14:13:26.00 Plunge.seek :locked @14:13:26.15 Air.in :fast @14:13:26.24 Air.seek :locked @14:13:27.24 Carousel.reconfigure :locked @14:13:27.42 Powering cartridge bus @14:13:30.08 Gate.setIR 1750,5000,0,6 @14:13:30.23 Gate.setIR 0,5000,0,6 @14:13:30.32 PuckHeater.configure :default @14:13:30.41 Gate.power :heat,:ON,limit to 5000mA @14:13:31.14 Mounted lysis Cartridge board#Q05052 @14:13:31.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:13:31.42 Gate.power :heat,:OFF @14:13:31.44 Gate.power :cartridge,:OFF @14:13:31.57 Carousel.in :default @14:13:31.73 Carousel.home.seek 34 @14:13:32.18 Air.in :unlocking @14:13:32.33 Air.seek :clear @14:13:33.36 Lysis1.in :unlocking @14:13:33.55 Lysis1.seek :clear @14:13:33.60 Plunge.in :unlocking @14:13:33.75 Plunge.seek :clear @14:13:35.03 Carousel.seek 48 @14:13:38.23 Lysis1.in :fast @14:13:38.32 Lysis1.seek :locked @14:13:40.96 Plunge.in :default @14:13:41.08 Plunge.seek :locked @14:13:41.26 Air.in :fast @14:13:41.33 Air.seek :locked @14:13:42.34 Carousel.reconfigure :locked @14:13:42.51 Powering cartridge bus @14:13:45.18 Gate.setIR 1750,5000,0,6 @14:13:45.31 Gate.setIR 0,5000,0,6 @14:13:45.39 PuckHeater.configure :default @14:13:45.48 Gate.power :heat,:ON,limit to 5000mA @14:13:46.18 Mounted lysis Cartridge board#Q05062 @14:13:46.24 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:13:46.46 Gate.power :heat,:OFF @14:13:46.48 Gate.power :cartridge,:OFF @14:13:46.59 Carousel.in :default @14:13:46.73 Carousel.home.seek 48 @14:13:47.22 Air.in :unlocking @14:13:47.36 Air.seek :clear @14:13:48.39 Lysis1.in :unlocking @14:13:48.55 Lysis1.seek :clear @14:13:48.62 Plunge.in :unlocking @14:13:49.20 Plunge.seek :clear @14:13:50.31 Carousel.seek 60 @14:13:53.14 Lysis1.in :fast @14:13:53.21 Lysis1.seek :locked @14:13:55.96 Plunge.in :default @14:13:56.02 Plunge.seek :locked @14:13:56.21 Air.in :fast @14:13:56.28 Air.seek :locked @14:13:57.28 Carousel.reconfigure :locked @14:13:57.47 Powering cartridge bus @14:14:00.13 Gate.setIR 1750,5000,0,6 @14:14:00.28 Gate.setIR 0,5000,0,6 @14:14:00.37 PuckHeater.configure :default @14:14:00.46 Gate.power :heat,:ON,limit to 5000mA @14:14:01.18 Mounted lysis Cartridge board#Q05051 @14:14:01.23 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @14:15:03.34 -> Exhaust.open; PB.open; TV.bypass; Archive.full @14:15:03.37 Exhaust.open @14:15:04.07 Bypass.open @14:15:04.84 Toroid.in :hold @14:15:04.93 Toroid.seek :bypass @14:15:06.63 Toroid.in :default @14:15:06.73 Toroid.seek :clear @14:15:08.41 Archive.seek :full @14:15:08.61 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1962uL @14:15:42.91 -> ArS @14:15:43.08 Slide::Error in bill -- Archive Syringe (ArS) Not yet Homed! @14:15:53.50 -> ArS.goHome false @14:16:05.39 -> ArS.clear @14:16:05.44 Archive.seek :clear @14:16:05.67 Slide::Error in bill -- Archive Syringe (ArS) notReady at 1961uL @14:16:20.77 -> ESP.ready! @14:16:21.63 Archive.in :default @14:16:21.72 Archive.home.jog 0 @14:16:21.85 Archive.in :default @14:16:21.93 Archive.home.jog 0 @14:16:22.07 Archive.in :homing @14:16:22.14 Archive.jog 11700 @14:16:28.14 Archive.setRawPosition! 50 @14:16:28.21 Archive.seek :home @14:16:28.51 Archive.in :default @14:16:28.87 Toroid.seek :clear @14:16:29.12 Carousel.in :default @14:16:29.26 Carousel.home.seek 60 @14:16:29.69 Carousel.reconfigure :locked @14:16:29.93 Gate.setIR 1750,5000,0,6 @14:16:30.06 Gate.setIR 0,5000,0,6 @14:16:30.15 PuckHeater.configure :default @14:16:30.23 Gate.power :heat,:ON,limit to 5000mA @14:16:30.93 Mounted lysis Cartridge board#Q05051 @14:16:31.02 Using default cartridge heat loss curve for HeatingAccelInCarousel Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is opened Bypass = Bypass (PB) is opened Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.16psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) full Lysis1 = Lysis Syringe #1 (LyS1) locked Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear PuckHeater = Puck Heater at 23.5C @14:16:39.05 -> Sampler.psi 3.53002415878535 @14:17:19.73 -> to 60 :microSPR_phyto @14:17:27.96 -> PP.cartridge @14:17:28.00 PinPort.in :locking @14:17:28.09 PinPort.seek :locked Handoff Pin Port (PP) cartridge @14:17:35.85 -> Handoff.blocked @14:17:35.87 Handoff.select :blocked Handoff Valve::Manifold selects blocked @14:17:43.29 -> Handoff.spr @14:17:43.32 Handoff.select :spr Handoff Valve::Manifold selects Analytical Module @14:17:48.87 -> Handoff.waste @14:17:48.90 Handoff.select :waste Handoff Valve::Manifold selects waste @14:18:06.21 -> Cmd.stop @14:18:06.34 Exhaust.close @14:18:06.38 Bypass.close @14:18:06.54 Handoff.select :close @14:18:07.24 Gate.power :heat,:OFF @14:18:07.26 Gate.power :cartridge,:OFF @14:18:07.39 PinPort.in :unlocking @14:18:07.47 PinPort.seek :clear @14:18:07.99 Carousel.in :default @14:18:08.14 Carousel.home.seek 60 @14:18:08.50 Plunge.seek :locked @14:18:08.78 Air.in :unlocking @14:18:08.92 Air.seek :clear @14:18:09.11 Lysis1.in :unlocking @14:18:09.28 Lysis1.seek :clear @14:18:10.81 Gate.power :analytic1,:OFF @14:18:10.83 Gate.power :motor,:OFF @14:18:10.87 Gate.power :core,:OFF @14:18:10.90 Gate.power :main,:OFF @14:18:10.94 Safely stopped and ready to power off :STOPPED @14:18:17.40 -> slot {[60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 55..52 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @14:22:56.81 -> PhytoSampler.xray @14:23:56.51 -> PhytoSampler.source=nil @14:24:15.25 -> PhytoSampler.bypassVolume=10 10 @14:31:53.77 -> PhytoSample.primeVolume=100 @14:31:53.80 NameError in bill -- uninitialized constant PhytoSample @14:32:08.56 -> PhytoSampler.primeVolume=100 100 @14:34:44.28 -> PhytoSampler.goalVolume=50 50 @14:35:04.25 -> Cartridge.reserve [60..1] 60 @14:35:08.71 -> slot {[60, 59, 57, 50, 48, 46, 44, 42, 40, 38, 36, 34, 32, 30, 28, 26, 24, 22, 20, 18, 16, 14, 12, 10, 8, 6, 4, 2, 1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}} @14:35:57.04 -> BACSampler.source=nil @14:35:57.08 NameError in bill -- uninitialized constant BACSampler @14:36:09.88 -> BACsampler.source=nil @14:36:42.79 -> BACsampler.bypassVolume=10 10 @14:36:57.17 -> BACsampler.primeVolume=100 100 @14:37:59.74 -> PhytoSampler.xray @14:38:34.34 -> BACsampler.xray @14:39:20.92 -> Cartridge.release [60,59] 2 @14:39:26.80 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @14:53:20.57 -> fire lysis # @14:53:20.80 Gate.power :sampler,:OFF,limit to 3500mA @14:53:20.83 Gate.power :skimmer,:OFF,limit to 3500mA @14:53:20.88 Gate.power :main,:ON,limit to 5000mA @14:53:20.92 Gate.power :analytic1,:ON,limit to 4000mA @14:53:21.07 Powering core bus @14:53:23.40 SPR Module 1, FW Rev. B, FPGA Rev. C @14:53:23.84 Plunge.configure :default @14:53:23.95 SamplePump.configure :default @14:53:24.06 Toroid.configure :default @14:53:24.17 Carousel.configure :default @14:53:24.86 Abs.configure :default @14:53:24.96 PhaseSeparator.configure :default @14:53:25.09 Archive.configure :default @14:53:25.20 Air.configure :default @14:53:25.28 Lysis1.configure :default @14:53:25.35 Lysis2.configure :default @14:53:25.46 PinPort.configure :default @14:53:25.54 All elf controllers running firmware version 4.92 @14:53:25.57 Gate.power :motor,:ON,limit to 5000mA @14:53:25.89 Plunge.home.jog 0 @14:53:26.67 PinPort.home.jog 0 @14:53:26.70 Lysis1.home.jog 0 @14:53:26.77 Air.home.jog 0 @14:53:26.81 Archive.home.jog 0 @14:53:26.85 Toroid.home.jog 0 @14:53:26.90 Lysis2.home.jog 0 @14:53:26.95 Carousel.home.jog 0 @14:53:27.83 Plunge.in :homing @14:53:27.86 Lysis2.in :default @14:53:28.14 Lysis2.home.jog 0 @14:53:28.18 Plunge.jog 1607 @14:53:28.53 Lysis2.in :homing @14:53:28.59 Air.in :homing @14:53:28.64 Toroid.in :homing @14:53:28.69 Archive.in :homing @14:53:28.77 PinPort.in :homing @14:53:28.87 Lysis1.in :homing @14:53:29.10 Lysis2.jog 11700 @14:53:29.16 Air.jog 1371 @14:53:29.23 Toroid.jog 619 @14:53:29.30 PinPort.jog -107 @14:53:29.34 Archive.jog 1392 @14:53:29.43 Lysis1.jog 1396 @14:53:29.92 PinPort.setRawPosition! 0 @14:53:29.94 Slide::Error in Archive_home -- Archive Syringe (ArS) hitLimit at 1969uL while jogging Archive Syringe (ArS) @14:53:30.00 Plunge.setRawPosition! 50 @14:53:30.12 Archive.in :default @14:53:30.20 PinPort.seek :home @14:53:30.28 Plunge.seek :home @14:53:30.38 Archive.home.jog 0 @14:53:30.71 Air.setRawPosition! 50 @14:53:30.76 PinPort.in :default @14:53:30.90 Archive.in :default @14:53:31.08 Air.seek :home @14:53:31.15 Archive.home.jog 0 @14:53:31.40 Lysis1.setRawPosition! 50 @14:53:31.49 Plunge.in :default @14:53:31.59 Archive.in :fullPowerHoming @14:53:31.62 Lysis1.seek :home @14:53:31.86 Archive.jog 11700 @14:53:32.01 Air.in :default @14:53:32.05 Toroid.setRawPosition! 0 @14:53:32.22 Lysis1.in :default @14:53:32.24 Toroid.seek :home @14:53:32.45 Toroid.in :default @14:53:35.37 Lysis2.setRawPosition! 50 @14:53:35.45 Lysis2.seek :home @14:53:35.76 Lysis2.in :default @14:53:56.28 Archive.setRawPosition! 50 @14:53:56.38 Archive.seek :home @14:53:56.88 Archive.in :default @14:53:57.02 Toroid.seek :clear @14:53:58.75 Carousel.in :default @14:53:59.35 Carousel.home.seek 60 @14:53:59.75 Selecting Cartridge 60 @14:54:00.10 Carousel.seek 60 @14:54:00.38 Lysis1.in :fast @14:54:00.48 Lysis1.seek :locked @14:54:03.14 Plunge.seek :locked @14:54:03.34 Air.in :fast @14:54:03.41 Air.seek :locked @14:54:04.41 Carousel.reconfigure :locked @14:54:04.59 Powering cartridge bus @14:54:07.26 Gate.setIR 1750,5000,0,6 @14:54:07.39 Gate.setIR 0,5000,0,6 @14:54:07.48 PuckHeater.configure :default @14:54:07.57 Gate.power :heat,:ON,limit to 5000mA @14:54:08.27 Mounted lysis Cartridge board#Q05051 @14:54:08.33 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:54:08.42 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 60" @14:54:08.79 Preparing to filter cartridge 60 @14:54:09.15 Air.in :default @14:54:09.35 Air.seek :sealed @14:54:09.39 Priming sample loop w/100ml, bypass w/10ml @14:54:09.48 SamplePump.setPosition! 0ml @14:54:09.60 Bypass.open @14:54:11.12 Intake.open @14:54:12.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:54:14.92 Vacuum tare is 52% of full scale at 23C (drifted 1%) @14:54:15.50 Abs.in :default @14:54:15.73 Abs.hold @14:54:17.96 SamplePump.in :start @14:54:18.18 SamplePump.seek 10ml,1:10 @14:54:18.61 SamplePump.configure :prime @14:54:21.49 SamplePump.stop @14:54:21.87 Intake.close @14:54:21.98 Bypass.close @14:54:23.14 Abs.stop @14:54:23.55 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.2ml @14:54:23.59 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.25ml" @14:54:23.69 BadNews.email "Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.2ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @14:54:23.74 upsync @14:54:34.11 upsync @14:55:49.88 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 59 => { Type: :microSPR_phyto, State: :dry}, 60 => { Type: :microSPR_phyto, State: :leakyToroid}} @14:56:23.51 -> slot 60 =>[:dry, :microSPR_phyto] {60 => { Type: :microSPR_phyto, State: :dry}} @14:56:27.84 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @14:56:40.19 -> fire lysis # @14:56:40.41 Selecting Cartridge 60 @14:56:40.84 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 60" @14:56:41.32 Preparing to filter cartridge 60 @14:56:41.95 Air.seek :sealed @14:56:42.20 Cmd::Error in detached from bill -- Cannot process while in PRIMING state @14:56:42.24 Priming sample loop w/100ml, bypass w/10ml @14:56:42.28 Waiting to Resume from Checkpoint "Selecting Cartridge 60" @14:56:42.32 SamplePump.setPosition! 0ml @14:56:42.44 Bypass.open @14:56:43.89 Intake.open @14:56:44.11 GoodNews.email "After filtering 0.0ml",Subject: "Processing microSPR_phyto cartridge in slot 60" @14:56:45.46 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:56:46.04 upsync @14:56:47.67 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from bill" @14:56:48.24 Vacuum tare is 53% of full scale at 24C (drifted 1%) @14:56:48.50 Abs.in :default @14:56:48.64 Abs.hold @14:56:49.08 SamplePump.in :start @14:56:49.20 SamplePump.seek 10ml,1:10 @14:56:49.70 SamplePump.configure :prime @14:56:49.76 upsync @14:56:52.87 upsync @14:57:01.80 Abs.stop @14:57:01.90 SamplePump.setPosition! 0ml @14:57:01.98 Exhaust.open @14:57:03.35 Bypass.close @14:57:04.93 Abs.in :default @14:57:05.04 Abs.hold @14:57:05.39 SamplePump.in :start @14:57:05.49 SamplePump.seek 100ml,7:10 @14:57:06.08 SamplePump.configure :prime @14:57:08.61 SamplePump.stop @14:57:08.70 Intake.close @14:57:08.80 Exhaust.close @14:57:09.63 Abs.stop @14:57:09.93 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 3.9psi vacuum after 2.2ml @14:57:09.95 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.24ml" @14:57:09.99 BadNews.email "Serious Leak or Stall -- 0.82ml/s made only 3.9psi vacuum after 2.2ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" @14:57:15.64 upsync @14:59:30.19 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 59 => { Type: :microSPR_phyto, State: :dry}, 60 => { Type: :microSPR_phyto, State: :leakyToroid}} @14:59:45.53 -> fire lysis # @14:59:45.73 Selecting Cartridge 59 @14:59:45.96 Gate.power :heat,:OFF @14:59:45.99 Gate.power :cartridge,:OFF @14:59:46.10 Carousel.in :default @14:59:46.25 Carousel.home.seek 60 @14:59:46.69 Air.in :unlocking @14:59:46.83 Air.seek :clear @14:59:48.33 Lysis1.in :unlocking @14:59:48.59 Lysis1.seek :clear @14:59:48.64 Plunge.in :unlocking @14:59:48.85 Plunge.seek :clear @14:59:50.07 Carousel.seek 59 @14:59:50.64 Lysis1.in :fast @14:59:50.72 Lysis1.seek :locked @14:59:53.37 Plunge.in :default @14:59:53.57 Plunge.seek :locked @14:59:53.70 Air.in :fast @14:59:53.78 Air.seek :locked @14:59:54.85 Carousel.reconfigure :locked @14:59:55.11 Powering cartridge bus @14:59:57.84 Gate.setIR 1750,5000,0,6 @14:59:57.99 Gate.setIR 0,5000,0,6 @14:59:58.11 PuckHeater.configure :default @14:59:58.26 Gate.power :heat,:ON,limit to 5000mA @14:59:59.13 Mounted lysis Cartridge board#Q05086 @14:59:59.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:59:59.37 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 59" @14:59:59.88 Preparing to filter cartridge 59 @15:00:00.72 Air.in :default @15:00:01.82 Priming sample loop w/100ml, bypass w/10ml @15:00:01.85 Air.seek :sealed @15:00:01.94 Cmd::Error in detached from bill#2 -- Cannot process while in PRIMING state @15:00:02.02 SamplePump.setPosition! 0ml @15:00:02.09 Waiting to Resume from Checkpoint "Selecting Cartridge 60" @15:00:02.24 Bypass.open @15:00:03.76 Intake.open @15:00:04.59 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:00:07.23 Vacuum tare is 52% of full scale at 24C (drifted 1%) @15:00:07.57 GoodNews.email "After filtering 0.0ml",Subject: "Processing microSPR_phyto cartridge in slot 60" @15:00:07.80 Abs.in :default @15:00:07.98 Abs.hold @15:00:08.48 SamplePump.in :start @15:00:08.58 SamplePump.seek 10ml,1:10 @15:00:09.16 SamplePump.configure :prime @15:00:12.86 upsync @15:00:14.50 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from bill#2" @15:00:18.02 upsync @15:00:20.99 upsync @15:00:21.16 Abs.stop @15:00:21.25 SamplePump.setPosition! 0ml @15:00:21.33 Exhaust.open @15:00:22.74 Bypass.close @15:00:24.29 Abs.in :default @15:00:24.40 Abs.hold @15:00:25.31 SamplePump.in :start @15:00:25.41 SamplePump.seek 100ml,7:10 @15:00:26.00 SamplePump.configure :prime @15:00:28.53 SamplePump.stop @15:00:28.62 Intake.close @15:00:28.73 Exhaust.close @15:00:29.48 Abs.stop @15:00:29.78 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.82ml/s made only 19.7psi vacuum after 2.2ml @15:00:29.80 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.24ml" @15:00:29.84 BadNews.email "Serious Leak or Stall -- 0.82ml/s made only 19.7psi vacuum after 2.2ml",Subject: "Cartridge::Sampler::Leaky in FILTERING" /suscript/connectNetwork: No such file or directory @15:00:46.56 Errno::ECONNRESET in Qmail -- Connection reset by peer @15:00:46.60 Retry #1 of 10 after 10:00 @15:00:46.67 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :leakyToroid}} @15:03:12.68 -> slot [60, 59] =>[:dry, :microSPR_phyto] {[60,59] => { Type: :microSPR_phyto, State: :dry}} @15:03:16.36 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @15:03:27.37 -> exit @15:03:27.39 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @15:04:09.24 Session Begun @15:04:14.52 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @15:04:30.85 -> fire lysis # @15:04:31.10 Selecting Cartridge 60 @15:04:31.34 Gate.power :heat,:OFF @15:04:31.37 Gate.power :cartridge,:OFF @15:04:31.49 Carousel.in :default @15:04:31.65 Carousel.home.seek 59 @15:04:32.12 Air.in :unlocking @15:04:32.28 Air.seek :clear @15:04:33.78 Lysis1.in :unlocking @15:04:33.96 Lysis1.seek :clear @15:04:34.09 Plunge.in :unlocking @15:04:34.24 Plunge.seek :clear @15:04:35.43 Carousel.seek 60 @15:04:36.00 Lysis1.in :fast @15:04:36.09 Lysis1.seek :locked @15:04:38.75 Plunge.in :default @15:04:38.84 Plunge.seek :locked @15:04:39.65 Air.in :fast @15:04:39.74 Air.seek :locked @15:04:40.74 Carousel.reconfigure :locked @15:04:40.93 Powering cartridge bus @15:04:43.60 Gate.setIR 1750,5000,0,6 @15:04:43.73 Gate.setIR 0,5000,0,6 @15:04:43.82 PuckHeater.configure :default @15:04:43.91 Gate.power :heat,:ON,limit to 5000mA @15:04:44.63 Mounted lysis Cartridge board#Q05051 @15:04:44.69 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:04:45.11 Preparing to filter cartridge 60 @15:04:45.59 Air.in :default @15:04:45.71 Air.seek :sealed @15:04:45.87 Priming sample loop w/100ml, bypass w/10ml @15:04:45.95 SamplePump.setPosition! 0ml @15:04:46.07 Bypass.open @15:04:46.20 Cmd::Error in detached from bill#3 -- Cannot process while in PRIMING state @15:04:46.22 Waiting to Resume from Checkpoint "Selecting Cartridge 59" @15:04:47.56 Intake.open @15:04:48.32 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:04:50.94 Vacuum tare is 53% of full scale at 24C (drifted 1%) @15:04:51.16 Abs.in :default @15:04:51.25 Abs.hold @15:04:51.52 SamplePump.in :start @15:04:51.62 SamplePump.seek 10ml,1:10 @15:04:52.21 SamplePump.configure :prime @15:05:04.17 Abs.stop @15:05:04.25 SamplePump.setPosition! 0ml @15:05:04.33 Exhaust.open @15:05:05.71 Bypass.close @15:05:07.28 Abs.in :default @15:05:07.37 Abs.hold @15:05:07.71 SamplePump.in :start @15:05:07.79 SamplePump.seek 100ml,7:10 @15:05:08.40 SamplePump.configure :prime @15:07:10.56 Abs.stop @15:07:10.76 Bypass.open @15:07:11.47 Intake.close @15:07:12.24 Toroid.in :hold @15:07:12.33 Toroid.seek :crack @15:07:13.92 Toroid.seek :cartridge @15:07:15.07 Toroid.in :default @15:07:15.16 Toroid.seek :clear @15:07:17.27 Pressurizing cartridge to 26psi above 14.9psia @15:07:17.29 SamplePump.setPosition! 0ml @15:07:17.38 Intake.open @15:07:18.08 Bypass.close @15:07:18.80 Exhaust.close @15:07:19.58 Abs.in :default @15:07:19.67 Abs.hold @15:07:19.93 SamplePump.in :charge @15:07:20.03 SamplePump.seek 2.3ml,42 seconds @15:07:34.31 Abs.stop @15:07:34.41 Intake.close @15:07:35.20 Pressurized cartridge with 0.917ml to 26.8psi @15:07:35.21 Isolated @39.7psia (24.8psi) @15:07:35.25 Verifying cartridge holds at least 0.65..0.85 of 39.7psia @15:07:35.32 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:07:39.95 Cartridge pressure fell 1% to 39.5psia @15:07:40.08 Sampling 50.0ml @15:07:40.10 SamplePump.setPosition! 0ml @15:07:40.19 Exhaust.open @15:07:40.87 Intake.open @15:07:41.80 Abs.in :default @15:07:41.89 Abs.hold @15:07:42.26 SamplePump.in :start @15:07:42.36 SamplePump.seek 50ml,4:40 @15:07:42.95 SamplePump.configure :sample @15:07:45.35 Cartridge 60 filtering 2/50ml at 0.56ml/s with 25.3psi vacuum @15:08:45.41 Cartridge 60 filtering 45/50ml at 0.72ml/s with 20.3psi vacuum 5.5m deep (22.8psia) @15:08:52.46 Abs.stop @15:08:53.21 Intake.close @15:08:53.32 Exhaust.close @15:08:53.60 Toroid.in :hold @15:08:53.70 Toroid.seek :bypass @15:08:55.36 Toroid.in :default @15:08:55.45 Toroid.seek :clear @15:08:57.10 Sampled 50.0ml @15:08:59.11 SPR.command "read_sequences" @15:08:59.62 SPR.command "stats_on" @15:08:59.81 SPR.command "start 0 1" @15:09:00.50 SPR.start /var/log/esp/spr24aug0115h.spr.bz2 @15:09:02.48 Handoff.select :waste @15:09:02.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:09:09.29 Syringe tare is 51% of full scale at 24C (drifted 0%) @15:09:09.32 PinPort.in :locking @15:09:09.44 PinPort.seek :locked @15:09:09.96 Archive.in :fast @15:09:10.03 Lysis2.in :fast @15:09:10.18 Lysis1.seek 1350uL @15:09:10.27 Lysis2.seek 950uL @15:09:10.33 Archive.seek :full @15:09:10.76 Slide::Error in posLyS2 -- IGNORED Lysis Syringe #2 (LyS2) hitLimit at 2002uL @15:09:10.79 Slide::Error in posArchive -- IGNORED Archive Syringe (ArS) hitLimit at 1962uL @15:09:10.85 Lysis2.in :default @15:09:10.89 Archive.in :default @15:09:11.05 Lysis2.hold @15:09:11.16 Archive.hold @15:09:11.35 Lysis2.in :default @15:09:11.39 Archive.in :default @15:09:11.55 Lysis2.home.jog 0 @15:09:11.61 Archive.home.jog 0 @15:09:11.87 Slide::Error in posLyS2 -- Lysis Syringe #2 (LyS2) hitLimit at 2002uL @15:09:11.92 Slide::Error in posArchive -- Archive Syringe (ArS) hitLimit at 1961uL @15:09:11.97 Waiting to Resume from Checkpoint "Lysis Syringe #2 (LyS2) hitLimit at 2002uL" @15:09:11.99 Waiting to Resume from Checkpoint "Archive Syringe (ArS) hitLimit at 1961uL" @15:10:50.30 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 60" @15:10:52.33 upsync @15:10:53.94 GoodNews.email "After filtering 0.0ml",Subject: "Processing microSPR_phyto cartridge in slot 59" @15:10:55.95 upsync @15:10:56.80 BadNews.email "Cannot process while in PRIMING state",Subject: "Cmd::Error in detached from bill#3" @15:10:58.73 upsync @15:10:59.97 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 60" @15:11:01.91 upsync @15:11:03.09 BadNews.email "Lysis Syringe #2 (LyS2) hitLimit at 2002uL",Subject: "Slide::Error in posLyS2" @15:11:05.04 upsync @15:11:06.22 BadNews.email "Archive Syringe (ArS) hitLimit at 1961uL",Subject: "Slide::Error in posArchive" @15:11:08.07 upsync @15:11:11.13 upsync @15:18:00.56 -> ArS @15:18:00.67 Slide::Error in bill -- Archive Syringe (ArS) Not yet Homed! @15:19:03.45 -> ArS.home.to :full @15:19:03.48 Archive.in :default @15:19:03.65 Archive.home.seek :full @15:19:03.79 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1961uL @15:19:25.38 -> ArS.home.to :full @15:19:25.43 Archive.in :default @15:19:25.51 Archive.home.seek :full @15:19:25.64 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1961uL @15:19:46.24 -> ArS.home.to :full @15:19:46.27 Archive.in :default @15:19:46.36 Archive.home.seek :full @15:19:46.50 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1961uL @15:20:45.59 -> ArS.goHome false @15:20:55.45 -> ArS.goHome! @15:20:55.48 Archive.in :default @15:20:55.58 Archive.home.jog 0 @15:20:55.71 Archive.in :default @15:20:55.81 Archive.home.jog 0 @15:20:55.95 Archive.in :homing @15:20:56.03 Archive.jog 11700 @15:21:02.06 Archive.setRawPosition! 50 @15:21:02.15 Archive.seek :home @15:21:02.46 Archive.in :default Archive Syringe (ArS) clear @15:21:16.68 -> ArS.full @15:21:16.73 Archive.seek :full @15:21:16.99 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1962uL @15:21:52.08 -> ArS.jog -100 @15:21:52.11 Archive.jog -100 @15:21:52.91 Slide::Error in bill -- Archive Syringe (ArS) Not yet Homed! @15:22:06.53 -> ArS.goHome! @15:22:06.57 Archive.in :default @15:22:06.65 Archive.home.jog 0 @15:22:06.79 Archive.in :default @15:22:06.88 Archive.home.jog 0 @15:22:07.11 Archive.in :homing @15:22:07.21 Archive.jog 11700 @15:22:13.21 Archive.setRawPosition! 50 @15:22:13.30 Archive.seek :home @15:22:13.60 Archive.in :default Archive Syringe (ArS) clear @15:22:43.10 -> ArS Archive Syringe (ArS) clear @15:23:19.44 -> ArS.goHome! @15:23:19.49 Archive.in :default @15:23:19.56 Archive.home.jog 0 @15:23:19.70 Archive.in :default @15:23:19.79 Archive.home.jog 0 @15:23:20.53 Archive.in :homing @15:23:20.62 Archive.jog 11700 @15:23:26.63 Archive.setRawPosition! 50 @15:23:26.71 Archive.seek :home @15:23:27.03 Archive.in :default Archive Syringe (ArS) clear @15:25:18.56 -> ArS.goHome! @15:25:18.60 Archive.in :default @15:25:18.69 Archive.home.jog 0 @15:25:19.35 Archive.in :default @15:25:19.55 Archive.home.jog 0 @15:25:19.69 Archive.in :homing @15:25:19.77 Archive.jog 11700 @15:25:25.77 Archive.setRawPosition! 50 @15:25:25.86 Archive.seek :home @15:25:26.16 Archive.in :default Archive Syringe (ArS) clear @15:25:47.51 -> ArS.goHome! @15:25:47.55 Archive.in :default @15:25:47.64 Archive.home.jog 0 @15:25:47.77 Archive.in :default @15:25:47.86 Archive.home.jog 0 @15:25:48.01 Archive.in :homing @15:25:48.10 Archive.jog 11700 @15:25:54.09 Archive.setRawPosition! 50 @15:25:54.18 Archive.seek :home @15:25:54.48 Archive.in :default Archive Syringe (ArS) clear @15:26:06.62 -> ArS.jog -100 @15:26:06.67 Archive.jog -100 @15:26:06.85 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1962uL @15:26:23.96 -> ArS.jog -100 @15:26:24.00 Archive.jog -100 @15:26:24.74 Slide::Error in bill -- Archive Syringe (ArS) Not yet Homed! @15:26:37.28 -> ArS.goHome! @15:26:37.32 Archive.in :default @15:26:37.40 Archive.home.jog 0 @15:26:37.57 Archive.in :default @15:26:37.69 Archive.home.jog 0 @15:26:38.42 Archive.in :homing @15:26:38.54 Archive.jog 11700 @15:26:44.54 Archive.setRawPosition! 50 @15:26:44.62 Archive.seek :home @15:26:44.92 Archive.in :default Archive Syringe (ArS) clear @15:26:52.63 -> ArS.jog -200 @15:26:52.69 Archive.jog -200 @15:26:52.91 Slide::Error in bill -- Archive Syringe (ArS) hitLimit at 1962uL @15:36:43.44 -> Cmd.stop @15:36:43.67 Handoff.select :close @15:36:43.87 Handoff.select :close @15:36:44.00 Gate.power :heat,:OFF @15:36:44.03 Gate.power :cartridge,:OFF @15:36:44.15 PinPort.in :unlocking @15:36:44.24 PinPort.seek :clear @15:36:44.43 PinPort.seek :clear @15:36:44.57 Slide::Error in detached from bill#4 -- Handoff Pin Port (PP) aborted at cartridge - 567 counts (sensed cartridge - 567 counts) @15:36:44.60 Retry #1 of 2 @15:36:57.49 Ended log spr24aug0115h.spr.bz2 @15:36:57.51 SPR.command "stats_off" @15:36:57.61 Carousel.in :default @15:36:57.82 Carousel.home.seek 60 @15:36:58.18 Plunge.seek :locked @15:36:58.38 Slide::Error in bill -- Archive Syringe (ArS) Not yet Homed! @15:36:58.41 Retry #1 of 2 @15:36:58.76 Gate.power :analytic1,:OFF @15:36:58.78 Gate.power :motor,:OFF @15:36:58.82 Gate.power :core,:OFF @15:36:58.87 Gate.power :main,:OFF @15:36:58.93 Gate.power :sampler,:OFF,limit to 3500mA @15:36:58.95 Gate.power :skimmer,:OFF,limit to 3500mA @15:36:59.08 Gate.power :sampler,:OFF,limit to 3500mA @15:36:59.10 Gate.power :skimmer,:OFF,limit to 3500mA @15:37:02.67 Gate.power :main,:ON,limit to 5000mA @15:37:02.70 Gate.power :analytic1,:ON,limit to 4000mA @15:37:02.81 Gate.power :analytic1,:ON,limit to 4000mA @15:37:03.45 Powering core bus @15:37:05.11 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 983 !! @15:37:05.16 Gate.power :core,:OFF @15:37:05.22 SPR Module 1, FW Rev. B, FPGA Rev. C @15:37:05.30 Powering core bus @15:37:05.67 BusOff in bill -- core bus was not powered while configuring HandoffValves @15:37:05.70 Retry #2 of 2 @15:37:05.77 Gate.power :analytic1,:OFF @15:37:05.80 Gate.power :core,:OFF @15:37:05.84 Gate.power :main,:OFF @15:37:05.90 Gate.power :sampler,:OFF,limit to 3500mA @15:37:05.93 Gate.power :skimmer,:OFF,limit to 3500mA @15:37:06.01 Powering core bus @15:37:08.77 Gate.power :motor,:ON,limit to 5000mA @15:37:08.80 AnalyticModule::Unknown in detached from bill#4 -- SPR @15:37:08.95 Slide::Error in detached from bill#4 -- Handoff Pin Port (PP) Not yet Homed! @15:37:08.98 Retry #2 of 2 @15:37:09.12 Gate.power :motor,:OFF @15:37:09.17 Gate.power :sampler,:OFF,limit to 3500mA @15:37:09.19 Gate.power :skimmer,:OFF,limit to 3500mA @15:37:09.64 Gate.power :main,:ON,limit to 5000mA @15:37:09.66 Gate.power :analytic1,:ON,limit to 4000mA @15:37:09.76 Gate.power :analytic1,:ON,limit to 4000mA @15:37:10.07 Plunge.configure :default @15:37:10.25 SamplePump.configure :default @15:37:10.42 Toroid.configure :default @15:37:10.60 Carousel.configure :default @15:37:10.77 Abs.configure :default @15:37:10.95 PhaseSeparator.configure :default @15:37:11.17 Archive.configure :default @15:37:11.34 Air.configure :default @15:37:11.50 Lysis1.configure :default @15:37:11.65 Lysis2.configure :default @15:37:12.17 Posix::Error in Probe-AMspr -- serial port AMspr already locked by process 983 !! @15:37:12.28 Gate.power :core,:OFF @15:37:12.31 SPR Module 1, FW Rev. B, FPGA Rev. C @15:37:12.39 Powering core bus @15:37:12.43 PinPort.configure :unlocking @15:37:12.51 BusOff in detached from bill#4 -- Handoff Pin Port (PP) core bus was not powered @15:37:12.58 Gate.power :analytic1,:OFF @15:37:12.60 Gate.power :core,:OFF @15:37:12.63 Gate.power :main,:OFF @15:37:12.66 ready to power off @15:37:12.88 Powering core bus @15:37:15.65 Gate.power :motor,:ON,limit to 5000mA @15:37:15.70 AnalyticModule::Unknown in bill -- SPR @15:37:15.84 Slide::Error in bill -- Handoff Pin Port (PP) Not yet Homed! @15:37:15.95 Gate.power :motor,:OFF @15:37:15.97 ready to power off :STOPPED @15:37:25.87 -> ls :ls @15:37:36.62 -> ESP.ready! @15:37:36.67 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @15:37:36.72 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @15:37:36.77 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @15:37:36.93 Slide::Error in bill -- Handoff Pin Port (PP) Not yet Homed! @15:37:41.99 upsync @15:37:42.69 -> ESP.reready! @15:37:42.80 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @15:37:42.88 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @15:37:42.96 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @15:37:43.07 Slide::Error in bill -- Handoff Pin Port (PP) Not yet Homed! @15:37:48.32 upsync @15:38:02.21 -> ESP.readay! @15:38:02.25 NoMethodError in bill -- undefined method `readay!' for ESP:Module @15:38:10.45 -> ESP.reready! @15:38:10.52 BusOff in clearToroid -- Toroid Valve (TV) is not powered! @15:38:10.61 Waiting to Resume from Checkpoint "Toroid Valve (TV) is not powered!" @15:38:10.69 BadNews.email "Toroid Valve (TV) is not powered!",Subject: "BusOff in clearToroid" @15:38:10.80 Slide::Error in bill -- Handoff Pin Port (PP) Not yet Homed! @15:38:15.34 upsync @15:38:25.26 -> PP.toHome! @15:38:25.30 Cartridge::TypeUnknown in bill -- No cartridge is mounted Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @18:30:55.66PDT01-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @18:30:57.44 >BREAK< ESP3 Gateway v1.43 for core bus @18:30:57.47 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:31:02.19 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @18:31:03.34 Sea pressure (Abs) tare is 6% of full scale @18:31:17.35 Polling Can every 2:00 @18:31:17.40 Waiting for espclients... @18:31:17.70 Session Begun @18:31:20.18 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 59 => { Type: :microSPR_phyto, State: :dry}, 60 => { Type: :lyseHold_phyto, State: :dry}} @18:31:59.50 -> slot 60 =>[:dry, :microSPR_phyto] {60 => { Type: :microSPR_phyto, State: :dry}} @18:32:04.18 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [58,56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @18:34:09.09 -> Cartridge.release 58 1 @18:34:11.96 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 58 => { Type: :archiveHiBiomass_bac, State: :dry}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @18:34:54.94 -> BACsampler.source=nil @18:35:11.59 -> PhytoSampler.source=nil @18:35:29.79 -> BACsampler.bypassVolume=10 10 @18:35:56.08 -> PhytoSampler.bypassVolume=10 10 @18:36:11.40 -> BACsampler.primeVolume=100 100 @18:36:22.58 -> PhytoSampler.primeVolume=100 100 @18:36:35.36 -> PhytoSampler.goalVolume=50 50 @18:36:47.38 -> BACsapler.xray @18:36:47.40 NameError in bill -- uninitialized constant BACsapler @18:36:55.58 -> BACsampler.xray @18:37:31.31 -> PhytoSampler.xray @18:37:56.15 -> ESP.ready! @18:37:56.23 Gate.power :sampler,:OFF,limit to 3500mA @18:37:56.25 Gate.power :skimmer,:OFF,limit to 3500mA @18:37:56.28 Gate.power :main,:ON,limit to 5000mA @18:37:56.31 Gate.power :analytic1,:ON,limit to 4000mA @18:37:56.53 Powering core bus @18:37:58.76 SPR Module 1, FW Rev. B, FPGA Rev. C @18:37:59.30 Plunge.configure :default @18:37:59.41 SamplePump.configure :default @18:37:59.52 Toroid.configure :default @18:37:59.63 Carousel.configure :default @18:37:59.74 Abs.configure :default @18:37:59.84 PhaseSeparator.configure :default @18:37:59.95 Archive.configure :default @18:38:00.05 Air.configure :default @18:38:00.16 Lysis1.configure :default @18:38:00.26 Lysis2.configure :default @18:38:00.37 PinPort.configure :default @18:38:00.46 All elf controllers running firmware version 4.92 @18:38:00.49 Gate.power :motor,:ON,limit to 5000mA @18:38:00.78 Plunge.home.jog 0 @18:38:01.47 Lysis2.home.jog 0 @18:38:01.54 Lysis1.home.jog 0 @18:38:01.59 Archive.home.jog 0 @18:38:01.63 Toroid.home.jog 0 @18:38:01.66 PinPort.home.jog 0 @18:38:01.69 Carousel.home.jog 0 @18:38:01.78 Air.home.jog 0 @18:38:03.27 Plunge.in :homing @18:38:03.48 Plunge.jog 1603 @18:38:03.56 PinPort.in :homing @18:38:03.61 Lysis2.in :homing @18:38:03.65 Air.in :homing @18:38:03.69 Toroid.in :homing @18:38:03.73 Lysis1.in :homing @18:38:03.78 Archive.in :homing @18:38:04.17 PinPort.jog -110 @18:38:04.23 Lysis2.jog 1399 @18:38:04.29 Lysis1.jog 1392 @18:38:04.33 Archive.jog 1389 @18:38:04.37 Toroid.jog 620 @18:38:04.41 Air.jog 1367 @18:38:04.85 PinPort.setRawPosition! 0 @18:38:04.93 PinPort.seek :home @18:38:05.04 PinPort.in :default @18:38:05.13 Plunge.setRawPosition! 50 @18:38:05.22 Plunge.seek :home @18:38:05.52 Plunge.in :default @18:38:05.70 Lysis2.setRawPosition! 50 @18:38:05.80 Lysis2.seek :home @18:38:05.94 Air.setRawPosition! 50 @18:38:05.97 Archive.setRawPosition! 50 @18:38:06.00 Lysis1.setRawPosition! 50 @18:38:06.20 Air.seek :home @18:38:06.28 Archive.seek :home @18:38:06.39 Lysis1.seek :home @18:38:06.63 Lysis2.in :default @18:38:06.69 Air.in :default @18:38:06.88 Archive.in :default @18:38:06.93 Toroid.setRawPosition! 0 @18:38:06.97 Lysis1.in :default @18:38:07.16 Toroid.seek :home @18:38:07.28 Toroid.in :default @18:38:07.42 Toroid.seek :clear @18:38:09.10 Carousel.in :default @18:38:09.25 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:38:55.11 -> to 1; to 15; to 34; to 48; to 60 @18:38:55.42 Carousel.seek 1 @18:39:07.78 Lysis1.in :fast @18:39:07.86 Lysis1.seek :locked @18:39:10.52 Plunge.seek :locked @18:39:10.71 Air.in :fast @18:39:10.78 Air.seek :locked @18:39:11.78 Carousel.reconfigure :locked @18:39:11.96 Powering cartridge bus @18:39:15.04 Gate.setIR 1750,5000,0,6 @18:39:15.17 Gate.setIR 0,5000,0,6 @18:39:15.36 PuckHeater.configure :default @18:39:15.45 Gate.power :heat,:ON,limit to 5000mA @18:39:16.26 Mounted lysis Cartridge board#Q05059 @18:39:16.32 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:39:16.56 Gate.power :heat,:OFF @18:39:16.58 Gate.power :cartridge,:OFF @18:39:16.69 Carousel.in :default @18:39:16.84 Carousel.home.seek 1 @18:39:17.30 Air.in :unlocking @18:39:17.44 Air.seek :clear @18:39:18.46 Lysis1.in :unlocking @18:39:18.63 Lysis1.seek :clear @18:39:18.68 Plunge.in :unlocking @18:39:18.85 Plunge.seek :clear @18:39:20.08 Carousel.seek 15 @18:39:23.30 Lysis1.in :fast @18:39:23.39 Lysis1.seek :locked @18:39:26.04 Plunge.in :default @18:39:26.12 Plunge.seek :locked @18:39:26.34 Air.in :fast @18:39:26.42 Air.seek :locked @18:39:27.43 Carousel.reconfigure :locked @18:39:27.60 Powering cartridge bus @18:39:30.30 Gate.setIR 1750,5000,0,6 @18:39:30.43 Gate.setIR 0,5000,0,6 @18:39:30.53 Gate.power :cartridge,:OFF @18:39:31.10 Mounted archive Cartridge @18:39:31.38 Carousel.in :default @18:39:31.52 Carousel.home.seek 15 @18:39:31.95 Air.in :unlocking @18:39:32.12 Air.seek :clear @18:39:33.19 Lysis1.in :unlocking @18:39:33.37 Lysis1.seek :clear @18:39:33.42 Plunge.in :unlocking @18:39:33.59 Plunge.seek :clear @18:39:34.83 Carousel.seek 34 @18:39:39.05 Lysis1.in :fast @18:39:39.13 Lysis1.seek :locked @18:39:41.77 Plunge.in :default @18:39:41.86 Plunge.seek :locked @18:39:42.04 Air.in :fast @18:39:42.12 Air.seek :locked @18:39:43.54 Carousel.reconfigure :locked @18:39:43.83 Powering cartridge bus @18:39:46.49 Gate.setIR 1750,5000,0,6 @18:39:46.62 Gate.setIR 0,5000,0,6 @18:39:46.71 PuckHeater.configure :default @18:39:46.80 Gate.power :heat,:ON,limit to 5000mA @18:39:47.50 Mounted lysis Cartridge board#Q05052 @18:39:47.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:39:47.77 Gate.power :heat,:OFF @18:39:47.81 Gate.power :cartridge,:OFF @18:39:47.90 Carousel.in :default @18:39:48.06 Carousel.home.seek 34 @18:39:48.50 Air.in :unlocking @18:39:48.66 Air.seek :clear @18:39:49.70 Lysis1.in :unlocking @18:39:49.84 Lysis1.seek :clear @18:39:49.97 Plunge.in :unlocking @18:39:50.07 Plunge.seek :clear @18:39:51.22 Carousel.seek 48 @18:39:54.42 Lysis1.in :fast @18:39:54.50 Lysis1.seek :locked @18:39:57.15 Plunge.in :default @18:39:57.26 Plunge.seek :locked @18:39:57.42 Air.in :fast @18:39:57.50 Air.seek :locked @18:39:58.50 Carousel.reconfigure :locked @18:39:58.67 Powering cartridge bus @18:40:01.34 Gate.setIR 1750,5000,0,6 @18:40:01.49 Gate.setIR 0,5000,0,6 @18:40:01.58 PuckHeater.configure :default @18:40:01.66 Gate.power :heat,:ON,limit to 5000mA @18:40:02.36 Mounted lysis Cartridge board#Q05062 @18:40:02.40 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:40:02.63 Gate.power :heat,:OFF @18:40:02.65 Gate.power :cartridge,:OFF @18:40:02.77 Carousel.in :default @18:40:02.92 Carousel.home.seek 48 @18:40:03.37 Air.in :unlocking @18:40:03.50 Air.seek :clear @18:40:04.51 Lysis1.in :unlocking @18:40:04.69 Lysis1.seek :clear @18:40:04.79 Plunge.in :unlocking @18:40:04.91 Plunge.seek :clear @18:40:06.10 Carousel.seek 60 @18:40:08.92 Lysis1.in :fast @18:40:09.01 Lysis1.seek :locked @18:40:12.20 Plunge.in :default @18:40:12.33 Plunge.seek :locked @18:40:12.48 Air.in :fast @18:40:12.55 Air.seek :locked @18:40:13.55 Carousel.reconfigure :locked @18:40:13.72 Powering cartridge bus @18:40:16.40 Gate.setIR 1750,5000,0,6 @18:40:16.53 Gate.setIR 0,5000,0,6 @18:40:16.62 PuckHeater.configure :default @18:40:16.70 Gate.power :heat,:ON,limit to 5000mA @18:40:17.40 Mounted lysis Cartridge board#Q05086 @18:40:17.46 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @18:41:07.10 -> Exhaust.open; PB.open; TV.bypas; Archive.full @18:41:07.12 Exhaust.open @18:41:07.80 Bypass.open @18:41:08.51 NoMethodError in bill -- undefined method `bypas' for Toroid:Slide @18:41:22.36 -> TV.bypass; Archive.full @18:41:22.52 Toroid.in :hold @18:41:22.62 Toroid.seek :bypass @18:41:24.30 Toroid.in :default @18:41:24.39 Toroid.seek :clear @18:41:26.03 Archive.seek :full Archive Syringe (ArS) full @18:41:38.43 -> Leak.psia 14.5874710678318 @18:42:00.21 -> Sampler.psi 3.56212422219048 @18:42:13.96 -> Intake.close; Exhaust.close; PB.close @18:42:13.99 Exhaust.close @18:42:14.66 Bypass.close Bypass (PB) is closed @18:42:20.67 -> Cmd.stop @18:42:20.80 Gate.power :heat,:OFF @18:42:20.83 Gate.power :cartridge,:OFF @18:42:20.96 Carousel.in :default @18:42:21.10 Carousel.home.seek 60 @18:42:21.48 Plunge.seek :locked @18:42:21.69 Archive.in :unlocking @18:42:21.86 Archive.seek :clear @18:42:21.94 Air.in :unlocking @18:42:22.17 Air.seek :clear @18:42:22.31 Lysis1.in :unlocking @18:42:22.51 Lysis1.seek :clear @18:42:24.03 Gate.power :analytic1,:OFF @18:42:24.06 Gate.power :motor,:OFF @18:42:24.10 Gate.power :core,:OFF @18:42:24.13 Gate.power :main,:OFF @18:42:24.18 Safely stopped and ready to power off :STOPPED @18:42:33.06 -> ESP.ready! @18:42:33.11 Gate.power :sampler,:OFF,limit to 3500mA @18:42:33.13 Gate.power :skimmer,:OFF,limit to 3500mA @18:42:33.20 Gate.power :main,:ON,limit to 5000mA @18:42:33.22 Gate.power :analytic1,:ON,limit to 4000mA @18:42:33.33 Powering core bus @18:42:35.67 SPR Module 1, FW Rev. B, FPGA Rev. C @18:42:36.15 Plunge.configure :default @18:42:36.28 SamplePump.configure :default @18:42:36.37 Toroid.configure :default @18:42:36.48 Carousel.configure :default @18:42:36.58 Abs.configure :default @18:42:36.69 PhaseSeparator.configure :default @18:42:36.79 Archive.configure :default @18:42:36.89 Air.configure :default @18:42:36.97 Lysis1.configure :default @18:42:37.05 Lysis2.configure :default @18:42:37.15 PinPort.configure :default @18:42:37.23 All elf controllers running firmware version 4.92 @18:42:37.25 Gate.power :motor,:ON,limit to 5000mA @18:42:37.45 Plunge.home.jog 0 @18:42:38.89 Lysis2.home.jog 0 @18:42:38.96 Air.home.jog 0 @18:42:39.00 Carousel.home.jog 0 @18:42:39.06 Archive.home.jog 0 @18:42:39.10 Toroid.home.jog 0 @18:42:39.14 Lysis1.home.jog 0 @18:42:39.19 PinPort.home.jog 0 @18:42:39.99 Plunge.in :homing @18:42:40.32 Plunge.jog 1596 @18:42:40.59 Lysis2.in :homing @18:42:40.65 Lysis1.in :homing @18:42:40.70 Archive.in :homing @18:42:40.73 Toroid.in :homing @18:42:40.78 PinPort.in :homing @18:42:40.82 Air.in :homing @18:42:41.10 Lysis2.jog 1404 @18:42:41.14 Lysis1.jog 1395 @18:42:41.20 Archive.jog 1397 @18:42:41.25 Toroid.jog 625 @18:42:41.30 PinPort.jog -109 @18:42:41.37 Air.jog 1368 @18:42:41.81 Plunge.setRawPosition! 50 @18:42:41.89 Plunge.seek :home @18:42:42.00 PinPort.setRawPosition! 0 @18:42:42.08 PinPort.seek :home @18:42:42.26 PinPort.in :default @18:42:42.30 Plunge.in :default @18:42:42.53 Lysis2.setRawPosition! 50 @18:42:42.61 Lysis2.seek :home @18:42:42.74 Lysis1.setRawPosition! 50 @18:42:42.79 Archive.setRawPosition! 50 @18:42:42.94 Lysis1.seek :home @18:42:42.98 Archive.seek :home @18:42:43.19 Air.setRawPosition! 50 @18:42:43.22 Lysis2.in :default @18:42:43.37 Air.seek :home @18:42:43.52 Lysis1.in :default @18:42:43.55 Archive.in :default @18:42:43.75 Air.in :default @18:42:43.88 Toroid.setRawPosition! 0 @18:42:43.95 Toroid.seek :home @18:42:44.07 Toroid.in :default @18:42:44.23 Toroid.seek :clear @18:42:45.96 Carousel.in :default @18:42:46.11 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:42:58.21 -> to 60 @18:42:58.52 Carousel.seek 60 @18:42:59.20 Lysis1.in :fast @18:42:59.28 Lysis1.seek :locked @18:43:02.03 Plunge.seek :locked @18:43:02.24 Air.in :fast @18:43:02.32 Air.seek :locked @18:43:03.33 Carousel.reconfigure :locked @18:43:03.51 Powering cartridge bus @18:43:06.20 Gate.setIR 1750,5000,0,6 @18:43:06.33 Gate.setIR 0,5000,0,6 @18:43:06.42 PuckHeater.configure :default @18:43:06.51 Gate.power :heat,:ON,limit to 5000mA @18:43:07.20 Mounted lysis Cartridge board#Q05086 @18:43:07.26 Using default cartridge heat loss curve for HeatingAccelInCarousel :microSPR_phyto @18:43:19.44 -> PP.cartridge @18:43:19.47 PinPort.in :locking @18:43:19.57 PinPort.seek :locked Handoff Pin Port (PP) cartridge @18:43:25.50 -> Handoff.blocked @18:43:25.53 Handoff.select :blocked Handoff Valve::Manifold selects blocked @18:43:30.79 -> Handoff.spr @18:43:30.84 Handoff.select :spr Handoff Valve::Manifold selects Analytical Module @18:43:36.22 -> Handoff.waste @18:43:36.24 Handoff.select :waste Handoff Valve::Manifold selects waste @18:43:40.80 -> Cmd.stop @18:43:40.93 Handoff.select :close @18:43:41.11 Gate.power :heat,:OFF @18:43:41.14 Gate.power :cartridge,:OFF @18:43:41.28 PinPort.in :unlocking @18:43:41.36 PinPort.seek :clear @18:43:41.87 Carousel.in :default @18:43:42.02 Carousel.home.seek 60 @18:43:42.38 Plunge.seek :locked @18:43:42.65 Air.in :unlocking @18:43:42.80 Air.seek :clear @18:43:42.94 Lysis1.in :unlocking @18:43:43.08 Lysis1.seek :clear @18:43:44.61 Gate.power :analytic1,:OFF @18:43:44.65 Gate.power :motor,:OFF @18:43:44.67 Gate.power :core,:OFF @18:43:44.72 Gate.power :main,:OFF @18:43:44.74 Safely stopped and ready to power off :STOPPED @18:45:22.24 -> slot {[57,50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [56,51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 58 => { Type: :archiveHiBiomass_bac, State: :dry}, [60,59] => { Type: :microSPR_phyto, State: :dry}} @19:05:53.61 -> fire lysis # @19:05:53.84 Gate.power :sampler,:OFF,limit to 3500mA @19:05:53.88 Gate.power :skimmer,:OFF,limit to 3500mA @19:05:53.92 Gate.power :main,:ON,limit to 5000mA @19:05:53.96 Gate.power :analytic1,:ON,limit to 4000mA @19:05:54.06 Powering core bus @19:05:56.40 SPR Module 1, FW Rev. B, FPGA Rev. C @19:05:56.84 Plunge.configure :default @19:05:56.94 SamplePump.configure :default @19:05:57.06 Toroid.configure :default @19:05:57.17 Carousel.configure :default @19:05:57.27 Abs.configure :default @19:05:57.38 PhaseSeparator.configure :default @19:05:57.47 Archive.configure :default @19:05:57.58 Air.configure :default @19:05:57.65 Lysis1.configure :default @19:05:57.73 Lysis2.configure :default @19:05:57.83 PinPort.configure :default @19:05:57.90 All elf controllers running firmware version 4.92 @19:05:57.93 Gate.power :motor,:ON,limit to 5000mA @19:05:58.87 Plunge.home.jog 0 @19:05:59.59 Lysis2.home.jog 0 @19:05:59.65 Air.home.jog 0 @19:05:59.69 Carousel.home.jog 0 @19:05:59.74 Archive.home.jog 0 @19:05:59.80 Toroid.home.jog 0 @19:05:59.85 PinPort.home.jog 0 @19:05:59.90 Lysis1.home.jog 0 @19:06:00.74 Plunge.in :homing @19:06:01.15 Plunge.jog 1597 @19:06:01.24 Lysis2.in :homing @19:06:01.29 Air.in :homing @19:06:01.34 Toroid.in :homing @19:06:01.38 PinPort.in :homing @19:06:01.43 Archive.in :homing @19:06:01.47 Lysis1.in :homing @19:06:01.80 Lysis2.jog 1404 @19:06:01.92 Air.jog 1366 @19:06:01.95 Toroid.jog 625 @19:06:01.99 Lysis1.jog 1390 @19:06:02.05 PinPort.jog -109 @19:06:02.11 Archive.jog 1393 @19:06:02.65 PinPort.setRawPosition! 0 @19:06:02.73 PinPort.seek :home @19:06:02.87 Plunge.setRawPosition! 50 @19:06:02.90 PinPort.in :default @19:06:03.04 Plunge.seek :home @19:06:03.35 Plunge.in :default @19:06:03.48 Lysis2.setRawPosition! 50 @19:06:03.50 Air.setRawPosition! 50 @19:06:03.66 Air.seek :home @19:06:03.69 Lysis2.seek :home @19:06:03.90 Archive.setRawPosition! 50 @19:06:03.96 Lysis1.setRawPosition! 50 @19:06:04.10 Archive.seek :home @19:06:04.15 Lysis1.seek :home @19:06:04.42 Lysis2.in :default @19:06:04.44 Air.in :default @19:06:04.67 Archive.in :default @19:06:04.70 Toroid.setRawPosition! 0 @19:06:04.77 Lysis1.in :default @19:06:04.94 Toroid.seek :home @19:06:05.07 Toroid.in :default @19:06:05.22 Toroid.seek :clear @19:06:06.95 Carousel.in :default @19:06:07.11 Carousel.home.seek 60 @19:06:07.42 Selecting Cartridge 60 @19:06:07.75 Carousel.seek 60 @19:06:08.01 Lysis1.in :fast @19:06:08.11 Lysis1.seek :locked @19:06:10.79 Plunge.seek :locked @19:06:10.99 Air.in :fast @19:06:11.08 Air.seek :locked @19:06:12.52 Carousel.reconfigure :locked @19:06:12.71 Powering cartridge bus @19:06:15.38 Gate.setIR 1750,5000,0,6 @19:06:15.53 Gate.setIR 0,5000,0,6 @19:06:15.62 PuckHeater.configure :default @19:06:15.73 Gate.power :heat,:ON,limit to 5000mA @19:06:16.43 Mounted lysis Cartridge board#Q05086 @19:06:16.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:06:16.58 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 60" @19:06:16.98 Preparing to filter cartridge 60 @19:06:17.29 Air.in :default @19:06:17.49 Air.seek :sealed @19:06:17.55 Priming sample loop w/100ml, bypass w/10ml @19:06:17.64 SamplePump.setPosition! 0ml @19:06:17.76 Bypass.open @19:06:19.24 Intake.open @19:06:20.13 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:06:22.80 Vacuum tare is 53% of full scale at 23C (drifted 1%) @19:06:22.91 upsync @19:06:23.05 Abs.in :default @19:06:23.13 Abs.hold @19:06:23.41 SamplePump.in :start @19:06:23.50 SamplePump.seek 10ml,1:10 @19:06:24.09 SamplePump.configure :prime @19:06:36.08 Abs.stop @19:06:36.19 SamplePump.setPosition! 0ml @19:06:36.26 Exhaust.open @19:06:37.67 Bypass.close @19:06:39.19 Abs.in :default @19:06:39.27 Abs.hold @19:06:39.61 SamplePump.in :start @19:06:39.71 SamplePump.seek 100ml,7:10 @19:06:40.30 SamplePump.configure :prime @19:08:42.10 Abs.stop @19:08:42.24 Bypass.open @19:08:43.40 Intake.close @19:08:44.18 Toroid.in :hold @19:08:44.28 Toroid.seek :crack @19:08:45.85 Toroid.seek :cartridge @19:08:46.98 Toroid.in :default @19:08:47.06 Toroid.seek :clear @19:08:49.18 Pressurizing cartridge to 26psi above 14.7psia @19:08:49.20 SamplePump.setPosition! 0ml @19:08:49.29 Intake.open @19:08:49.96 Bypass.close @19:08:50.62 Exhaust.close @19:08:51.44 Abs.in :default @19:08:51.52 Abs.hold @19:08:51.79 SamplePump.in :charge @19:08:51.88 SamplePump.seek 2.3ml,42 seconds @19:09:05.37 Abs.stop @19:09:05.48 Intake.close @19:09:06.26 Pressurized cartridge with 0.816ml to 26.1psi @19:09:06.28 Isolated @38.1psia (23.4psi) @19:09:06.31 Verifying cartridge holds at least 0.65..0.85 of 38.1psia @19:09:06.38 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:09:11.08 Sampling 50.0ml @19:09:11.09 SamplePump.setPosition! 0ml @19:09:11.21 Exhaust.open @19:09:11.90 Intake.open @19:09:12.78 Abs.in :default @19:09:12.86 Abs.hold @19:09:13.20 SamplePump.in :start @19:09:13.31 SamplePump.seek 50ml,4:40 @19:09:13.90 SamplePump.configure :sample @19:09:16.30 Cartridge 60 filtering 2/50ml at 0.80ml/s with 23.3psi vacuum @19:10:16.36 Cartridge 60 filtering 46/50ml at 0.73ml/s with 25.4psi vacuum @19:10:22.17 Abs.stop @19:10:22.37 Intake.close @19:10:22.44 Exhaust.close @19:10:22.68 Toroid.in :hold @19:10:22.77 Toroid.seek :bypass @19:10:25.04 Toroid.in :default @19:10:25.12 Toroid.seek :clear @19:10:26.77 Sampled 50.0ml @19:10:27.23 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 60" @19:10:28.86 SPR.command "read_sequences" @19:10:29.64 SPR.command "stats_on" @19:10:29.83 SPR.command "start 0 1" @19:10:30.54 SPR.start /var/log/esp/spr24aug0119h.spr.bz2 @19:10:32.17 upsync @19:10:32.49 Handoff.select :waste @19:10:32.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:10:39.29 Syringe tare is 51% of full scale at 24C (drifted 0%) @19:10:39.33 PinPort.in :locking @19:10:39.44 PinPort.seek :locked @19:10:39.96 Archive.in :fast @19:10:40.02 Lysis2.in :fast @19:10:40.15 Lysis1.seek 1350uL @19:10:40.26 Archive.seek :full @19:10:40.41 Lysis2.seek 950uL @19:10:45.33 Plunge.seek :reagent @19:10:46.35 Evacuating puck with filter bubble point of 24psi @19:10:46.38 PinPort.seek :locked @19:10:46.54 Handoff.select :waste @19:10:46.60 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:10:53.67 Air syringe pumping up to 1 strokes to reach 8psi @19:10:53.68 Air stroke #1 of 1 @19:10:53.88 Air.in :pressurize @19:10:53.96 Air.seek :pressurized @19:10:59.60 0.7psi after pressurize @19:10:59.62 Air.in :compress @19:10:59.73 Air.seek :evac @19:11:02.78 1.7psi after compress @19:11:13.37 0.2psi after compress (stable) @19:11:13.39 Air.in :default @19:11:13.50 Air.hold @19:11:13.80 at 0.1psi Opened to waste @19:11:13.82 Handoff.select :blocked @19:11:14.01 Archive syringe pumping up to 8 strokes to reach 24psi @19:11:14.02 Archive stroke #1 of 8 @19:11:14.17 Archive.in :pressurize @19:11:14.27 Archive.seek :pressurized @19:11:26.72 6.9psi after pressurize @19:11:32.32 6.3psi after pressurize (stable) @19:11:32.35 Archive.in :compress @19:11:32.47 Archive.seek :empty @19:11:48.26 20.2psi after compress @19:12:00.35 21.7psi after compress (stable) @19:12:00.38 Archive.in :gulp @19:12:00.96 Archive.seek :topVent @19:12:07.40 22.9psi after gulp @19:12:12.99 22.4psi after gulp (stable) @19:12:13.01 Archive stroke #2 of 8 @19:12:13.16 Archive.in :pressurize @19:12:13.26 Archive.seek :pressurized @19:12:25.72 22.5psi after pressurize @19:12:31.31 21.8psi after pressurize (stable) @19:12:31.34 Archive.in :compress @19:12:31.45 Archive.seek :empty @19:12:40.13 Reached 24.2 of 24.0psi after pumping 3309uL (1.91 strokes) @19:12:40.18 Archive.in :default @19:12:40.29 Archive.hold @19:12:40.40 Handoff.select :waste @19:12:40.58 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:12:52.68 delay 1:00 @19:13:52.70 Handoff.select :blocked @19:13:52.87 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:14:15.48 Handoff.select :waste @19:14:15.65 0.3psi at evacEnd @19:14:15.67 Handoff.select :blocked @19:14:15.84 Archive.in :fast @19:14:15.92 Archive.seek :topVent @19:14:22.58 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @19:14:29.17 Stable @0.2psi after Handoff blocked @19:14:29.21 Archive syringe pumping up to 8 strokes to reach 24psi @19:14:29.23 Archive stroke #1 of 8 @19:14:29.39 Archive.in :pressurize @19:14:29.51 Archive.seek :pressurized @19:14:41.97 1.1psi after pressurize @19:14:42.00 Archive.in :compress @19:14:42.10 Archive.seek :empty @19:14:57.92 15.2psi after compress @19:15:08.51 14.3psi after compress (stable) @19:15:08.54 Archive.in :gulp @19:15:08.65 Archive.seek :topVent @19:15:15.50 14.6psi after gulp @19:15:21.09 14.5psi after gulp (stable) @19:15:21.11 Archive stroke #2 of 8 @19:15:21.25 Archive.in :pressurize @19:15:21.33 Archive.seek :pressurized @19:15:33.85 15.0psi after pressurize @19:15:33.88 Archive.in :compress @19:15:33.99 Archive.seek :empty @19:15:46.50 Reached 24.4 of 24.2psi after pumping 3390uL (1.96 strokes) @19:15:46.52 Archive.in :default @19:15:46.63 Archive.hold @19:15:46.73 Handoff.select :waste @19:15:46.89 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:15:54.00 delay 1:00 @19:16:54.02 Handoff.select :blocked @19:16:54.20 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:17:16.79 Handoff.select :waste @19:17:16.97 0.5psi at evacEnd @19:17:17.00 Air.seek :fullVent @19:17:18.36 Archive.in :fast @19:17:18.45 Archive.seek :topVent @19:17:25.39 Seawater evacuated, Pressure above/below filter released. @19:17:25.54 Lysis1.in :slow @19:17:25.63 Lysis1.seek 1050uL @19:17:55.28 delay 5 seconds @19:18:00.30 Handoff.select :blocked @19:18:00.56 Lysis1.seek 450uL @19:18:59.71 delay 3 seconds @19:19:02.85 Air.in mix with 1.57127 parts Lysis1 @19:19:03.57 Air.seek :evac @19:19:03.62 Lysis1.seek 300uL @19:19:18.65 Lysis1.seek :empty @19:19:48.35 Lysis buffer delivered @12.4psi @19:19:48.37 Polling Can every 2:00 @19:19:48.95 PuckHeater.configure :default #ambient 24.0C @19:19:49.03 PuckHeater.seek 79.7C @19:20:37.02 PuckHeater.hold 2:00,79.7C @19:22:36.69 Polling Can every 2:00 @19:22:37.24 PuckHeater.configure :default #ambient 24.0C @19:22:37.33 PuckHeater.hold 30 seconds,78.6C @19:23:07.44 Polling Can every 2:00 @19:23:07.68 PuckHeater.hold 30 seconds,78.1C @19:23:37.83 Polling Can every 2:00 @19:23:38.38 PuckHeater.configure :default #ambient 24.0C @19:23:39.03 PuckHeater.hold 10:00,77.6C @19:33:37.26 Lysis complete. Syringe pressure @16.5psi @19:33:37.29 Archive syringe pumping up to 5 strokes to reach 40psi @19:33:37.33 Archive stroke #1 of 5 @19:33:37.46 Archive.in :pressurize @19:33:37.54 Archive.seek :pressurized @19:33:50.00 16.8psi after pressurize @19:33:55.64 16.2psi after pressurize (stable) @19:33:55.68 Archive.in :compress @19:33:55.82 Archive.seek :empty @19:34:06.90 Reached 40.5 of 40.0psi after pumping 1630uL (0.94 strokes) @19:34:06.92 Archive.in :default @19:34:07.03 Archive.hold @19:34:07.65 delay 2 seconds @19:34:09.77 Archive.in :fast @19:34:09.85 Archive.seek :full @19:34:16.79 Lysis1.seek 138uL @19:34:30.51 delay 1 second @19:34:31.54 Archive.seek :full @19:34:31.65 Lysis1.seek :empty @19:34:45.44 Handoff.select :blocked @19:34:45.60 Initial air pressure @42.8psi @19:34:45.70 Lysis1.in :extract @19:34:45.82 Lysis1.seek 600uL,nil @19:34:50.44 Archive syringe pumping up to 6 strokes to reach 25psi @19:34:50.46 Archive stroke #1 of 6 @19:34:50.68 Archive.in :pressurize @19:34:50.78 Archive.seek :pressurized @19:34:51.38 Archive.in :default @19:34:51.46 Archive.hold @19:34:51.87 Initial 600uL lysate @14.2psi @19:34:51.88 Handoff.select :blocked @19:34:52.03 Initial air pressure @13.6psi @19:34:52.80 Lysis1.seek 825uL,nil @19:34:52.95 Archive syringe pumping up to 6 strokes to reach 17psi @19:34:52.96 Archive stroke #1 of 6 @19:34:53.21 Archive.in :pressurize @19:34:53.34 Archive.seek :pressurized @19:35:05.17 14.1psi after pressurize @19:35:10.81 13.2psi after pressurize (stable) @19:35:10.87 Archive.in :compress @19:35:11.03 Archive.seek :empty @19:35:16.27 Reached 15.8 of 17.0psi after pumping 1503uL (0.87 strokes) @19:35:16.29 Archive.in :default @19:35:16.37 Archive.hold @19:35:16.72 Archive syringe pumping up to 6 strokes to reach 17psi @19:35:16.74 Archive stroke #1 of 6 @19:35:16.89 Archive.in :compress @19:35:17.00 Archive.seek :empty @19:35:20.44 Archive.in :default @19:35:20.52 Archive.hold @19:35:20.87 Recovered 825uL lysate @16.5psi @19:35:20.90 Air.in :halfSpeed @19:35:21.00 Air.seek :fullVent @19:35:23.04 Puck Top Vented, bottom @17.9psi @19:35:23.05 Handoff.select :waste @19:35:23.22 delay 5 seconds @19:35:28.30 Handoff open to waste, bottom @0.4psi @19:35:28.32 Air.in :default @19:35:28.46 Air.seek :evac @19:35:30.36 Lysis2.in :DAmix @19:35:30.66 Lysis1.in :DAmix @19:35:31.26 Lysis2.seek 700uL @19:35:31.34 Lysis1.seek 575uL @19:36:05.97 delay 5 seconds @19:36:10.99 Handoff.select SPR @19:36:11.15 SPR.command "start 2 14" @19:36:12.37 SPR stabilized @19:36:12.55 delay 10 seconds @19:36:22.57 SPR.command "start 2 0" @19:36:25.73 Waiting up to 11:00 for SPR to enter state 1 @19:38:23.39 SPR.command "hold_on" @19:38:24.79 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:38:25.51 Lysis2.seek 400uL @19:38:25.57 Lysis1.seek 275uL @19:39:07.11 delay 15 seconds @19:39:22.14 SPR.command "hold_off" @19:39:25.95 Waiting up to 1:00:00 for SPR to enter state 7 @19:55:41.94 SPRsummary:-1.09e+03RIU,918RIU,128RIU,-3.45RIU,none,none,none after 2864s @20:09:58.84 SPR analysis completed @20:10:07.35 Ended log spr24aug0119h.spr.bz2 @20:10:07.38 SPR.command "stats_off" @20:10:07.99 Handoff.select :close @20:10:08.20 Gate.power :heat,:OFF @20:10:08.23 Gate.power :cartridge,:OFF @20:10:08.34 PinPort.in :unlocking @20:10:08.41 PinPort.seek :clear @20:10:08.92 Carousel.in :default @20:10:09.06 Carousel.home.seek 60 @20:10:09.42 Plunge.seek :locked @20:10:10.33 Archive.in :unlocking @20:10:10.49 Archive.seek :clear @20:10:10.62 Air.in :unlocking @20:10:10.77 Air.seek :clear @20:10:10.91 Lysis1.in :unlocking @20:10:11.08 Lysis1.seek :clear @20:10:11.66 Lysis2.in :unlocking @20:10:11.81 Lysis2.seek :clear @20:10:15.78 Gate.power :analytic1,:OFF @20:10:15.80 Gate.power :motor,:OFF @20:10:15.84 Gate.power :core,:OFF @20:10:15.88 Gate.power :main,:OFF @20:10:15.91 Safely stopped and ready to power off @20:10:26.42 -> fire lysis, archive # @20:10:26.65 Gate.power :sampler,:OFF,limit to 3500mA @20:10:26.71 Gate.power :skimmer,:OFF,limit to 3500mA @20:10:26.75 Gate.power :main,:ON,limit to 5000mA @20:10:26.78 Gate.power :analytic1,:ON,limit to 4000mA @20:10:26.90 Powering core bus @20:10:29.25 SPR Module 1, FW Rev. B, FPGA Rev. C @20:10:29.68 Plunge.configure :default @20:10:29.79 SamplePump.configure :default @20:10:29.90 Toroid.configure :default @20:10:30.01 Carousel.configure :default @20:10:30.11 Abs.configure :default @20:10:30.26 PhaseSeparator.configure :default @20:10:30.37 Archive.configure :default @20:10:30.48 Air.configure :default @20:10:30.56 Lysis1.configure :default @20:10:30.64 Lysis2.configure :default @20:10:30.75 PinPort.configure :default @20:10:30.83 All elf controllers running firmware version 4.92 @20:10:30.87 Gate.power :motor,:ON,limit to 5000mA @20:10:31.09 Plunge.home.jog 0 @20:10:31.95 Lysis2.home.jog 0 @20:10:32.00 Air.home.jog 0 @20:10:32.06 Carousel.home.jog 0 @20:10:32.11 Toroid.home.jog 0 @20:10:32.18 Lysis1.home.jog 0 @20:10:32.23 Archive.home.jog 0 @20:10:32.27 PinPort.home.jog 0 @20:10:33.20 Plunge.in :homing @20:10:33.43 Plunge.jog 1607 @20:10:33.65 Toroid.in :homing @20:10:33.73 Lysis2.in :homing @20:10:33.78 Air.in :homing @20:10:33.82 PinPort.in :homing @20:10:33.88 Archive.in :homing @20:10:33.92 Lysis1.in :homing @20:10:34.20 Toroid.jog 616 @20:10:34.33 Lysis2.jog 1403 @20:10:34.48 Air.jog 1365 @20:10:34.52 Archive.jog 1400 @20:10:34.59 PinPort.jog -109 @20:10:34.64 Lysis1.jog 1397 @20:10:35.04 Plunge.setRawPosition! 50 @20:10:35.13 Plunge.seek :home @20:10:35.25 PinPort.setRawPosition! 0 @20:10:35.34 PinPort.seek :home @20:10:35.50 Plunge.in :default @20:10:35.53 PinPort.in :default @20:10:36.27 Lysis2.setRawPosition! 50 @20:10:36.39 Lysis1.setRawPosition! 50 @20:10:36.44 Archive.setRawPosition! 50 @20:10:36.48 Air.setRawPosition! 50 @20:10:36.71 Lysis2.seek :home @20:10:36.79 Lysis1.seek :home @20:10:36.83 Archive.seek :home @20:10:36.88 Air.seek :home @20:10:37.24 Toroid.setRawPosition! 0 @20:10:37.36 Toroid.seek :home @20:10:37.57 Lysis1.in :default @20:10:37.61 Air.in :default @20:10:37.64 Toroid.in :default @20:10:37.69 Lysis2.in :default @20:10:37.73 Archive.in :default @20:10:38.28 Toroid.seek :clear @20:10:39.98 Carousel.in :default @20:10:40.16 Carousel.home.seek 60 @20:10:40.46 Selecting Cartridge 59 @20:10:40.78 Carousel.seek 59 @20:10:41.34 Lysis1.in :fast @20:10:41.43 Lysis1.seek :locked @20:10:44.08 Plunge.seek :locked @20:10:44.31 Air.in :fast @20:10:44.39 Air.seek :locked @20:10:45.39 Carousel.reconfigure :locked @20:10:45.56 Powering cartridge bus @20:10:48.22 Gate.setIR 1750,5000,0,6 @20:10:48.36 Gate.setIR 0,5000,0,6 @20:10:48.45 PuckHeater.configure :default #ambient 24.0C @20:10:48.54 Gate.power :heat,:ON,limit to 5000mA @20:10:49.24 Mounted lysis Cartridge board#Q05060 @20:10:49.30 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:10:49.39 GoodNews.email "PhytoSampler filtering up to 50 ml",Subject: "Sampling microSPR_phyto cartridge in slot 59" @20:10:49.75 Preparing to filter cartridge 59 @20:10:50.01 Air.in :default @20:10:50.20 Air.seek :sealed @20:10:50.38 Priming sample loop w/100ml, bypass w/10ml @20:10:50.40 SamplePump.setPosition! 0ml @20:10:50.51 Bypass.open @20:10:52.44 Intake.open @20:10:53.40 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:10:55.95 upsync @20:10:58.54 Vacuum tare is 53% of full scale at 24C (drifted 1%) @20:10:58.71 Abs.in :default @20:10:58.79 Abs.hold @20:10:59.07 SamplePump.in :start @20:10:59.17 SamplePump.seek 10ml,1:10 @20:10:59.76 SamplePump.configure :prime @20:11:11.73 Abs.stop @20:11:11.82 SamplePump.setPosition! 0ml @20:11:11.89 Exhaust.open @20:11:13.28 Bypass.close @20:11:14.85 Abs.in :default @20:11:14.94 Abs.hold @20:11:15.28 SamplePump.in :start @20:11:15.38 SamplePump.seek 100ml,7:10 @20:11:15.97 SamplePump.configure :prime @20:13:17.77 Abs.stop @20:13:17.89 Bypass.open @20:13:18.60 Intake.close @20:13:19.40 Toroid.in :hold @20:13:19.48 Toroid.seek :crack @20:13:21.06 Toroid.seek :cartridge @20:13:22.23 Toroid.in :default @20:13:22.32 Toroid.seek :clear @20:13:24.42 Pressurizing cartridge to 26psi above 15.2psia @20:13:24.45 SamplePump.setPosition! 0ml @20:13:24.54 Intake.open @20:13:25.21 Bypass.close @20:13:25.91 Exhaust.close @20:13:26.81 Abs.in :default @20:13:26.89 Abs.hold @20:13:27.16 SamplePump.in :charge @20:13:27.25 SamplePump.seek 2.3ml,42 seconds @20:13:41.84 Abs.stop @20:13:41.94 Intake.close @20:13:42.69 Pressurized cartridge with 0.954ml to 26.6psi @20:13:42.71 Isolated @39.1psia (23.9psi) @20:13:42.73 Verifying cartridge holds at least 0.65..0.85 of 39.1psia @20:13:42.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:13:47.41 Cartridge pressure fell 1% to 38.6psia @20:13:47.54 Sampling 50.0ml @20:13:47.56 SamplePump.setPosition! 0ml @20:13:47.65 Exhaust.open @20:13:48.31 Intake.open @20:13:49.16 Abs.in :default @20:13:49.25 Abs.hold @20:13:49.59 SamplePump.in :start @20:13:49.70 SamplePump.seek 50ml,4:40 @20:13:50.29 SamplePump.configure :sample @20:13:52.70 Cartridge 59 filtering 2/50ml at 0.72ml/s with 19.6psi vacuum @20:14:52.74 Cartridge 59 filtering 44/50ml at 0.71ml/s with 18.5psi vacuum @20:15:00.73 Abs.stop @20:15:00.91 Intake.close @20:15:01.02 Exhaust.close @20:15:01.26 Toroid.in :hold @20:15:01.35 Toroid.seek :bypass @20:15:03.02 Toroid.in :default @20:15:03.11 Toroid.seek :clear @20:15:04.76 Sampled 50.0ml @20:15:05.21 GoodNews.email "After filtering 50.0ml",Subject: "Processing microSPR_phyto cartridge in slot 59" @20:15:07.51 SPR.command "read_sequences" @20:15:08.16 SPR.command "stats_on" @20:15:08.36 SPR.command "start 0 1" @20:15:09.08 SPR.start /var/log/esp/spr24aug0120h.spr.bz2 @20:15:10.49 upsync @20:15:11.01 Handoff.select :waste @20:15:11.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:15:17.82 Syringe tare is 51% of full scale at 25C (drifted 0%) @20:15:17.85 PinPort.in :locking @20:15:17.96 PinPort.seek :locked @20:15:18.49 Archive.in :fast @20:15:18.56 Lysis2.in :fast @20:15:18.63 Lysis1.seek 1350uL @20:15:18.82 Archive.seek :full @20:15:18.92 Lysis2.seek 950uL @20:15:23.82 Plunge.seek :reagent @20:15:24.82 Evacuating puck with filter bubble point of 24psi @20:15:24.86 PinPort.seek :locked @20:15:25.00 Handoff.select :waste @20:15:25.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:15:31.66 Air syringe pumping up to 1 strokes to reach 8psi @20:15:31.67 Air stroke #1 of 1 @20:15:31.81 Air.in :pressurize @20:15:31.89 Air.seek :pressurized @20:15:37.53 1.4psi after pressurize @20:15:37.56 Air.in :compress @20:15:37.66 Air.seek :evac @20:15:40.71 3.7psi after compress @20:15:56.30 0.5psi after compress (stable) @20:15:56.31 Air.in :default @20:15:56.42 Air.hold @20:15:56.67 at 0.5psi Opened to waste @20:15:56.68 Handoff.select :blocked @20:15:56.87 Archive syringe pumping up to 8 strokes to reach 24psi @20:15:56.90 Archive stroke #1 of 8 @20:15:57.04 Archive.in :pressurize @20:15:57.13 Archive.seek :pressurized @20:16:09.58 4.8psi after pressurize @20:16:21.67 8.5psi after pressurize (stable) @20:16:21.70 Archive.in :compress @20:16:21.82 Archive.seek :empty @20:16:37.58 15.6psi after compress @20:16:48.23 14.7psi after compress (stable) @20:16:48.26 Archive.in :gulp @20:16:48.37 Archive.seek :topVent @20:16:54.71 15.4psi after gulp @20:17:00.34 14.4psi after gulp (stable) @20:17:00.36 Archive stroke #2 of 8 @20:17:00.51 Archive.in :pressurize @20:17:00.59 Archive.seek :pressurized @20:17:13.04 15.2psi after pressurize @20:17:24.15 19.5psi after pressurize (stable) @20:17:24.21 Archive.in :compress @20:17:24.31 Archive.seek :empty @20:17:33.74 Reached 24.1 of 24.0psi after pumping 3324uL (1.92 strokes) @20:17:33.76 Archive.in :default @20:17:33.86 Archive.hold @20:17:33.97 Handoff.select :waste @20:17:34.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:17:45.75 delay 1:00 @20:18:45.77 Handoff.select :blocked @20:18:46.00 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @20:19:08.76 Handoff.select :waste @20:19:08.92 0.8psi at evacEnd @20:19:08.93 Handoff.select :blocked @20:19:09.10 Archive.in :fast @20:19:09.19 Archive.seek :topVent @20:19:15.91 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @20:19:22.50 Stable @0.4psi after Handoff blocked @20:19:22.54 Archive syringe pumping up to 8 strokes to reach 24psi @20:19:22.56 Archive stroke #1 of 8 @20:19:22.71 Archive.in :pressurize @20:19:22.80 Archive.seek :pressurized @20:19:35.25 4.3psi after pressurize @20:19:35.28 Archive.in :compress @20:19:35.41 Archive.seek :empty @20:19:51.15 15.2psi after compress @20:20:01.75 14.1psi after compress (stable) @20:20:01.78 Archive.in :gulp @20:20:01.92 Archive.seek :topVent @20:20:08.27 14.9psi after gulp @20:20:13.86 14.1psi after gulp (stable) @20:20:13.87 Archive stroke #2 of 8 @20:20:14.03 Archive.in :pressurize @20:20:14.12 Archive.seek :pressurized @20:20:26.64 14.7psi after pressurize @20:20:26.68 Archive.in :compress @20:20:26.78 Archive.seek :empty @20:20:38.11 Reached 23.5 of 24.4psi after pumping 3354uL (1.94 strokes) @20:20:38.25 Archive.in :default @20:20:38.36 Archive.hold @20:20:38.46 Handoff.select :waste @20:20:38.63 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:20:45.73 delay 1:00 @20:21:45.76 Handoff.select :blocked @20:21:45.94 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @20:22:08.53 Handoff.select :waste @20:22:08.72 0.6psi at evacEnd @20:22:08.75 Air.seek :fullVent @20:22:10.10 Archive.in :fast @20:22:10.23 Archive.seek :topVent @20:22:17.05 Seawater evacuated, Pressure above/below filter released. @20:22:17.21 Lysis1.in :slow @20:22:17.31 Lysis1.seek 1050uL @20:22:46.97 delay 5 seconds @20:22:51.99 Handoff.select :blocked @20:22:52.25 Lysis1.seek 450uL @20:23:51.42 delay 3 seconds @20:23:54.54 Air.in mix with 1.57127 parts Lysis1 @20:23:55.33 Air.seek :evac @20:23:55.46 Lysis1.seek 300uL @20:24:10.44 Lysis1.seek :empty @20:24:40.21 Lysis buffer delivered @12.4psi @20:24:40.23 Polling Can every 2:00 @20:24:40.80 PuckHeater.configure :default #ambient 24.8C @20:24:40.91 PuckHeater.seek 79.6C @20:25:30.99 PuckHeater.hold 2:00,79.6C @20:27:30.63 Polling Can every 2:00 @20:27:31.18 PuckHeater.configure :default #ambient 24.9C @20:27:31.27 PuckHeater.hold 30 seconds,78.6C @20:28:01.40 Polling Can every 2:00 @20:28:01.97 PuckHeater.hold 30 seconds,78.1C @20:28:32.08 Polling Can every 2:00 @20:28:33.20 PuckHeater.hold 10:00,77.6C @20:38:30.93 Lysis complete. Syringe pressure @4.5psi @20:38:30.97 Archive syringe pumping up to 5 strokes to reach 40psi @20:38:31.01 Archive stroke #1 of 5 @20:38:31.14 Archive.in :pressurize @20:38:31.22 Archive.seek :pressurized @20:38:43.73 8.1psi after pressurize @20:39:07.33 21.7psi after pressurize (stable) @20:39:07.36 Archive.in :compress @20:39:07.46 Archive.seek :empty @20:39:19.03 Reached 40.5 of 40.0psi after pumping 1640uL (0.95 strokes) @20:39:19.06 Archive.in :default @20:39:19.16 Archive.hold @20:39:19.26 delay 2 seconds @20:39:21.29 Archive.in :fast @20:39:21.37 Archive.seek :full @20:39:28.34 Lysis1.seek 138uL @20:39:42.59 delay 1 second @20:39:43.74 Archive.seek :full @20:39:43.83 Lysis1.seek :empty @20:39:57.62 Handoff.select :blocked @20:39:57.75 Initial air pressure @39.9psi @20:39:57.84 Lysis1.in :extract @20:39:57.98 Lysis1.seek 600uL,nil @20:40:02.25 Archive syringe pumping up to 6 strokes to reach 25psi @20:40:02.27 Archive stroke #1 of 6 @20:40:02.47 Archive.in :pressurize @20:40:02.59 Archive.seek :pressurized @20:40:03.73 Archive.in :default @20:40:03.81 Archive.hold @20:40:04.23 Initial 600uL lysate @13.8psi @20:40:04.25 Handoff.select :blocked @20:40:04.41 Initial air pressure @13.2psi @20:40:04.53 Lysis1.seek 825uL,nil @20:40:04.72 Archive syringe pumping up to 6 strokes to reach 17psi @20:40:04.74 Archive stroke #1 of 6 @20:40:04.96 Archive.in :pressurize @20:40:05.07 Archive.seek :pressurized @20:40:16.42 16.6psi after pressurize @20:40:16.85 Reached 17.3 of 17.0psi after pumping 1397uL (0.81 strokes) @20:40:16.87 Archive.in :default @20:40:16.97 Archive.hold @20:40:17.82 Archive syringe pumping up to 6 strokes to reach 17psi @20:40:17.83 Archive stroke #1 of 6 @20:40:17.96 Archive.in :compress @20:40:18.06 Archive.seek :empty @20:40:18.62 Reached 17.1 of 17.0psi after pumping 1402uL (0.81 strokes) @20:40:18.64 Archive.in :default @20:40:18.74 Archive.hold @20:40:19.35 Recovered 825uL lysate @16.3psi @20:40:19.38 Air.in :halfSpeed @20:40:19.49 Air.seek :fullVent @20:40:21.53 Puck Top Vented, bottom @18.0psi @20:40:21.55 Handoff.select :waste @20:40:21.73 delay 5 seconds @20:40:26.80 Handoff open to waste, bottom @0.4psi @20:40:26.83 Air.in :default @20:40:26.93 Air.seek :evac @20:40:28.27 Lysis2.in :DAmix @20:40:29.02 Lysis1.in :DAmix @20:40:29.65 Lysis2.seek 700uL @20:40:29.70 Lysis1.seek 575uL @20:41:04.34 delay 5 seconds @20:41:09.36 Handoff.select SPR @20:41:09.52 SPR.command "start 2 14" @20:41:11.20 SPR stabilized @20:41:11.39 delay 10 seconds @20:41:21.41 SPR.command "start 2 0" @20:41:24.56 Waiting up to 11:00 for SPR to enter state 1 @20:43:22.65 SPR.command "hold_on" @20:43:24.05 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:43:24.80 Lysis2.seek 400uL @20:43:24.86 Lysis1.seek 275uL @20:44:06.39 delay 15 seconds @20:44:21.41 SPR.command "hold_off" @20:44:24.70 Waiting up to 1:00:00 for SPR to enter state 7 @21:00:40.27 SPRsummary:4.39RIU,144RIU,150RIU,4.62RIU,44ng/L,none,high after 2885s @21:14:57.18 SPR analysis completed @21:15:06.29 Ended log spr24aug0120h.spr.bz2 @21:15:06.33 SPR.command "stats_off" @21:15:07.18 Selecting Cartridge 58 @21:15:07.38 Gate.power :heat,:OFF @21:15:07.40 Gate.power :cartridge,:OFF @21:15:07.52 PinPort.in :unlocking @21:15:07.61 PinPort.seek :clear @21:15:08.11 Carousel.in :default @21:15:08.30 Carousel.home.seek 59 @21:15:08.67 Archive.in :unlocking @21:15:08.86 Air.in :unlocking @21:15:08.89 Archive.seek :clear @21:15:09.07 Air.seek :clear @21:15:09.24 Lysis2.in :unlocking @21:15:09.33 Lysis2.seek :clear @21:15:13.82 Lysis1.in :unlocking @21:15:13.97 Lysis1.seek :clear @21:15:14.10 Plunge.in :unlocking @21:15:14.22 Plunge.seek :clear @21:15:17.92 Carousel.seek 58 @21:15:18.49 Lysis1.in :fast @21:15:18.56 Lysis1.seek :locked @21:15:21.20 Plunge.in :default @21:15:21.33 Plunge.seek :locked @21:15:21.48 Air.in :fast @21:15:21.56 Air.seek :locked @21:15:22.56 Carousel.reconfigure :locked @21:15:22.73 Powering cartridge bus @21:15:25.40 Gate.setIR 1750,5000,0,6 @21:15:25.54 Gate.setIR 0,5000,0,6 @21:15:25.64 Gate.power :cartridge,:OFF @21:15:26.22 Mounted archive Cartridge @21:15:26.30 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 58" @21:15:26.67 Preparing to filter cartridge 58 @21:15:27.04 Priming sample loop w/100ml, bypass w/10ml @21:15:27.09 SamplePump.setPosition! 0ml @21:15:27.18 Bypass.open @21:15:28.70 Intake.open @21:15:29.64 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:15:32.18 upsync @21:15:34.86 Vacuum tare is 53% of full scale at 25C (drifted 2%) @21:15:35.04 Abs.in :default @21:15:35.62 Abs.hold @21:15:35.98 SamplePump.in :start @21:15:36.09 SamplePump.seek 10ml,1:10 @21:15:36.67 SamplePump.configure :prime @21:15:48.69 Abs.stop @21:15:48.78 SamplePump.setPosition! 0ml @21:15:48.86 Exhaust.open @21:15:50.29 Bypass.close @21:15:51.85 Abs.in :default @21:15:51.94 Abs.hold @21:15:52.32 SamplePump.in :start @21:15:52.43 SamplePump.seek 100ml,7:10 @21:15:53.01 SamplePump.configure :prime @21:17:54.75 Abs.stop @21:17:54.85 Bypass.open @21:17:55.55 Intake.close @21:17:56.33 Toroid.in :hold @21:17:56.42 Toroid.seek :crack @21:17:57.99 Toroid.seek :cartridge @21:17:59.14 Toroid.in :default @21:17:59.22 Toroid.seek :clear @21:18:01.27 Pressurizing cartridge to 26psi above 15.2psia @21:18:01.30 SamplePump.setPosition! 0ml @21:18:01.39 Intake.open @21:18:02.05 Bypass.close @21:18:02.74 Exhaust.close @21:18:03.51 Abs.in :default @21:18:03.59 Abs.hold @21:18:03.84 SamplePump.in :charge @21:18:03.94 SamplePump.seek 2.3ml,42 seconds @21:18:13.05 Abs.stop @21:18:13.15 Intake.close @21:18:13.89 Pressurized cartridge with 0.370ml to 26.8psi @21:18:13.91 Isolated @40.4psia (25.3psi) @21:18:13.93 Verifying cartridge holds at least 0.65..0.85 of 40.4psia @21:18:14.02 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:18:18.78 Cartridge pressure fell 1% to 40.1psia @21:18:18.90 Sampling 1000.0ml @21:18:18.92 SamplePump.setPosition! 0ml @21:18:19.01 Exhaust.open @21:18:19.64 Intake.open @21:18:20.51 Abs.in :default @21:18:20.58 Abs.hold @21:18:20.94 SamplePump.in :start @21:18:21.04 SamplePump.seek 1000ml,1:23:50 @21:18:21.63 SamplePump.configure :sample @21:18:24.03 Cartridge 58 filtering 2/1000ml at 0.59ml/s with 26.4psi vacuum @21:19:24.09 Cartridge 58 filtering 42/1000ml at 0.67ml/s with 23.4psi vacuum @21:20:24.09 Cartridge 58 filtering 81/1000ml at 0.65ml/s with 28.8psi vacuum @21:21:24.09 Cartridge 58 filtering 119/1000ml at 0.64ml/s with 20.0psi vacuum @21:22:24.09 Cartridge 58 filtering 157/1000ml at 0.63ml/s with 21.8psi vacuum 3.8m deep (20.4psia) @21:23:24.08 Cartridge 58 filtering 194/1000ml at 0.62ml/s with 20.2psi vacuum @21:24:24.09 Cartridge 58 filtering 231/1000ml at 0.62ml/s with 18.1psi vacuum 5.9m deep (23.5psia) @21:25:24.09 Cartridge 58 filtering 268/1000ml at 0.61ml/s with 19.6psi vacuum @21:26:24.09 Cartridge 58 filtering 304/1000ml at 0.61ml/s with 29.9psi vacuum @21:27:24.09 Cartridge 58 filtering 340/1000ml at 0.60ml/s with 22.3psi vacuum @21:28:24.09 Cartridge 58 filtering 376/1000ml at 0.60ml/s with 27.6psi vacuum @21:29:24.09 Cartridge 58 filtering 412/1000ml at 0.59ml/s with 25.9psi vacuum @21:30:24.09 Cartridge 58 filtering 447/1000ml at 0.59ml/s with 30.7psi vacuum @21:31:24.09 Cartridge 58 filtering 482/1000ml at 0.58ml/s with 21.8psi vacuum @21:32:24.09 Cartridge 58 filtering 516/1000ml at 0.58ml/s with 22.3psi vacuum 5.8m deep (23.3psia) @21:33:24.08 Cartridge 58 filtering 551/1000ml at 0.57ml/s with 22.3psi vacuum 6.5m deep (24.3psia) @21:34:24.09 Cartridge 58 filtering 585/1000ml at 0.57ml/s with 26.8psi vacuum @21:35:24.08 Cartridge 58 filtering 619/1000ml at 0.57ml/s with 28.4psi vacuum @21:36:24.09 Cartridge 58 filtering 653/1000ml at 0.56ml/s with 30.8psi vacuum @21:37:24.08 Cartridge 58 filtering 687/1000ml at 0.56ml/s with 25.2psi vacuum @21:38:24.09 Cartridge 58 filtering 720/1000ml at 0.56ml/s with 30.8psi vacuum @21:39:24.09 Cartridge 58 filtering 753/1000ml at 0.55ml/s with 30.7psi vacuum @21:40:24.10 Cartridge 58 filtering 785/1000ml at 0.54ml/s with 23.3psi vacuum @21:41:24.09 Cartridge 58 filtering 818/1000ml at 0.54ml/s with 22.4psi vacuum @21:42:24.09 Cartridge 58 filtering 850/1000ml at 0.54ml/s with 20.9psi vacuum @21:43:24.08 Cartridge 58 filtering 883/1000ml at 0.54ml/s with 14.8psi vacuum 6.8m deep (24.7psia) @21:44:24.08 Cartridge 58 filtering 915/1000ml at 0.54ml/s with 26.2psi vacuum @21:45:24.09 Cartridge 58 filtering 947/1000ml at 0.53ml/s with 26.5psi vacuum @21:46:24.09 Cartridge 58 filtering 979/1000ml at 0.54ml/s with 31.5psi vacuum @21:47:03.07 Abs.stop @21:47:03.24 Intake.close @21:47:03.36 Exhaust.close @21:47:03.45 Handoff.select :close @21:47:03.68 Toroid.in :hold @21:47:03.77 Toroid.seek :bypass @21:47:05.43 Toroid.in :default @21:47:05.52 Toroid.seek :clear @21:47:07.16 Sampled 1000.0ml @21:47:07.62 GoodNews.email "After filtering 1000.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 58" @21:47:08.22 Plunge.seek :reagent @21:47:09.08 Sample loop closed. Ready to Deliver Reagents @21:47:09.11 Archive.in :default @21:47:09.22 Archive.seek :sealed @21:47:12.57 upsync @21:47:13.18 Archive.seek :evac @21:47:27.46 delay 1:00 @21:48:27.50 Archive.seek :clear @21:48:45.22 RNAlater soak for 10:00 @21:58:45.26 Archive.seek :tVent @21:58:47.81 delay 1 second @21:58:48.84 Archive.seek :evac @21:59:04.51 delay 10 seconds @21:59:14.55 Archive.seek :tVent @21:59:30.21 delay 1 second @21:59:31.24 Archive.seek :evac @21:59:46.92 delay 10 seconds @21:59:56.95 Sample preserved and evacuated @21:59:57.30 Carousel.in :default @21:59:57.50 Carousel.home.seek 58 @21:59:58.38 Plunge.seek :locked @21:59:59.40 Archive.in :unlocking @21:59:59.54 Archive.seek :clear @21:59:59.68 Air.in :unlocking @21:59:59.83 Air.seek :clear @21:59:59.96 Lysis1.in :unlocking @22:00:00.10 Lysis1.seek :clear @22:00:04.19 Gate.power :analytic1,:OFF @22:00:04.23 Gate.power :motor,:OFF @22:00:04.26 Gate.power :core,:OFF @22:00:04.29 Gate.power :main,:OFF @22:00:04.32 Safely stopped and ready to power off @22:00:23.52 -> Cartridge.release 57 1 @22:00:33.89 -> Cartridge.release 56 1 @22:00:37.62 -> slot {[50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 56 => { Type: :archiveHiBiomass_bac, State: :dry}, 57 => { Type: :microSPR_phyto, State: :dry}, 58 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0120h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0119h.spr.bz2}} @22:01:30.39 -> PhytoSampler.goalVolume=1000 1000 @22:01:44.70 -> PhytoSampler.xray @22:02:37.18 -> fire lysis, archive # @22:02:37.43 Gate.power :sampler,:OFF,limit to 3500mA @22:02:37.45 Gate.power :skimmer,:OFF,limit to 3500mA @22:02:37.50 Gate.power :main,:ON,limit to 5000mA @22:02:37.53 Gate.power :analytic1,:ON,limit to 4000mA @22:02:37.64 Powering core bus @22:02:39.98 SPR Module 1, FW Rev. B, FPGA Rev. C @22:02:40.42 Plunge.configure :default @22:02:40.53 SamplePump.configure :default @22:02:40.64 Toroid.configure :default @22:02:40.74 Carousel.configure :default @22:02:40.86 Abs.configure :default @22:02:40.97 PhaseSeparator.configure :default @22:02:41.07 Archive.configure :default @22:02:41.17 Air.configure :default @22:02:41.25 Lysis1.configure :default @22:02:41.33 Lysis2.configure :default @22:02:41.44 PinPort.configure :default @22:02:41.52 All elf controllers running firmware version 4.92 @22:02:41.55 Gate.power :motor,:ON,limit to 5000mA @22:02:41.75 Plunge.home.jog 0 @22:02:42.52 Archive.home.jog 0 @22:02:42.67 Lysis2.home.jog 0 @22:02:42.73 Air.home.jog 0 @22:02:42.78 Toroid.home.jog 0 @22:02:42.84 Lysis1.home.jog 0 @22:02:42.88 Carousel.home.jog 0 @22:02:42.94 PinPort.home.jog 0 @22:02:43.83 Plunge.in :homing @22:02:44.11 Plunge.jog 1597 @22:02:44.22 Lysis2.in :homing @22:02:44.28 Lysis1.in :homing @22:02:44.33 PinPort.in :homing @22:02:44.38 Archive.in :homing @22:02:44.42 Toroid.in :homing @22:02:44.48 Air.in :homing @22:02:45.38 Lysis2.jog 1406 @22:02:45.41 Lysis1.jog 1395 @22:02:45.52 PinPort.jog -111 @22:02:45.58 Archive.jog 1395 @22:02:45.72 Air.jog 1359 @22:02:45.81 Toroid.jog 626 @22:02:46.21 Plunge.setRawPosition! 50 @22:02:46.24 PinPort.setRawPosition! 0 @22:02:46.37 Plunge.seek :home @22:02:46.43 PinPort.seek :home @22:02:46.68 PinPort.in :default @22:02:46.81 Plunge.in :default @22:02:46.91 Lysis2.setRawPosition! 50 @22:02:46.96 Lysis1.setRawPosition! 50 @22:02:47.13 Lysis1.seek :home @22:02:47.16 Lysis2.seek :home @22:02:47.38 Archive.setRawPosition! 50 @22:02:47.42 Air.setRawPosition! 50 @22:02:47.58 Air.seek :home @22:02:47.61 Archive.seek :home @22:02:47.88 Lysis1.in :default @22:02:47.90 Lysis2.in :default @22:02:48.09 Air.in :default @22:02:48.18 Archive.in :default @22:02:48.36 Toroid.setRawPosition! 0 @22:02:48.43 Toroid.seek :home @22:02:48.57 Toroid.in :default @22:02:48.71 Toroid.seek :clear @22:02:50.45 Carousel.in :default @22:02:50.61 Carousel.home.seek 58 @22:02:50.89 Selecting Cartridge 57 @22:02:51.21 Carousel.seek 57 @22:02:51.79 Lysis1.in :fast @22:02:51.88 Lysis1.seek :locked @22:02:54.53 Plunge.seek :locked @22:02:54.72 Air.in :fast @22:02:54.79 Air.seek :locked @22:02:55.79 Carousel.reconfigure :locked @22:02:55.96 Powering cartridge bus @22:02:58.62 Gate.setIR 1750,5000,0,6 @22:02:58.75 Gate.setIR 0,5000,0,6 @22:02:58.84 PuckHeater.configure :default #ambient 24.9C @22:02:58.94 Gate.power :heat,:ON,limit to 5000mA @22:02:59.62 Mounted lysis Cartridge board#Q05075 @22:02:59.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:02:59.77 GoodNews.email "PhytoSampler filtering up to 1000 ml",Subject: "Sampling microSPR_phyto cartridge in slot 57" @22:03:00.78 Preparing to filter cartridge 57 @22:03:01.11 Air.in :default @22:03:01.26 Air.seek :sealed @22:03:01.38 Priming sample loop w/100ml, bypass w/10ml @22:03:01.42 SamplePump.setPosition! 0ml @22:03:01.51 Bypass.open @22:03:03.00 Intake.open @22:03:03.84 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:03:05.20 upsync @22:03:06.46 Vacuum tare is 53% of full scale at 26C (drifted 2%) @22:03:06.65 Abs.in :default @22:03:06.73 Abs.hold @22:03:07.02 SamplePump.in :start @22:03:07.12 SamplePump.seek 10ml,1:10 @22:03:07.71 SamplePump.configure :prime @22:03:19.69 Abs.stop @22:03:19.79 SamplePump.setPosition! 0ml @22:03:19.87 Exhaust.open @22:03:21.22 Bypass.close @22:03:22.80 Abs.in :default @22:03:22.88 Abs.hold @22:03:23.24 SamplePump.in :start @22:03:23.34 SamplePump.seek 100ml,7:10 @22:03:23.92 SamplePump.configure :prime @22:05:25.75 Abs.stop @22:05:25.86 Bypass.open @22:05:26.56 Intake.close @22:05:27.36 Toroid.in :hold @22:05:27.45 Toroid.seek :crack @22:05:29.03 Toroid.seek :cartridge @22:05:30.20 Toroid.in :default @22:05:30.29 Toroid.seek :clear @22:05:32.90 Pressurizing cartridge to 26psi above 15.6psia @22:05:32.92 SamplePump.setPosition! 0ml @22:05:33.02 Intake.open @22:05:33.69 Bypass.close @22:05:34.36 Exhaust.close @22:05:35.13 Abs.in :default @22:05:35.21 Abs.hold @22:05:35.48 SamplePump.in :charge @22:05:35.58 SamplePump.seek 2.3ml,42 seconds @22:05:49.37 Abs.stop @22:05:49.47 Intake.close @22:05:50.24 Pressurized cartridge with 0.854ml to 26.3psi @22:05:50.26 Isolated @38.9psia (23.3psi) @22:05:50.30 Verifying cartridge holds at least 0.65..0.85 of 38.9psia @22:05:50.36 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @22:05:54.97 Cartridge pressure fell 1% to 38.4psia @22:05:55.10 Sampling 1000.0ml @22:05:55.12 SamplePump.setPosition! 0ml @22:05:55.22 Exhaust.open @22:05:55.83 Intake.open @22:05:56.72 Abs.in :default @22:05:56.80 Abs.hold @22:05:57.16 SamplePump.in :start @22:05:57.27 SamplePump.seek 1000ml,1:23:50 @22:05:57.85 SamplePump.configure :sample @22:06:00.29 Cartridge 57 filtering 2/1000ml at 0.57ml/s with 23.6psi vacuum @22:07:00.34 Cartridge 57 filtering 42/1000ml at 0.67ml/s with 22.5psi vacuum @22:08:00.35 Cartridge 57 filtering 82/1000ml at 0.67ml/s with 20.0psi vacuum @22:09:00.35 Cartridge 57 filtering 122/1000ml at 0.66ml/s with 18.5psi vacuum 5.8m deep (23.3psia) @22:10:00.35 Cartridge 57 filtering 162/1000ml at 0.66ml/s with 20.6psi vacuum @22:11:00.35 Cartridge 57 filtering 201/1000ml at 0.66ml/s with 33.1psi vacuum @22:12:00.35 Cartridge 57 filtering 241/1000ml at 0.66ml/s with 21.7psi vacuum @22:12:41.04 Session Ended @22:13:00.35 Cartridge 57 filtering 280/1000ml at 0.66ml/s with 30.7psi vacuum @22:14:00.80 Cartridge 57 filtering 320/1000ml at 0.66ml/s with 22.3psi vacuum @22:15:00.35 Cartridge 57 filtering 359/1000ml at 0.66ml/s with 29.6psi vacuum @22:16:00.35 Cartridge 57 filtering 399/1000ml at 0.66ml/s with 20.7psi vacuum @22:17:00.35 Cartridge 57 filtering 438/1000ml at 0.66ml/s with 13.9psi vacuum 3.6m deep (20.0psia) @22:18:00.35 Cartridge 57 filtering 478/1000ml at 0.66ml/s with 15.2psi vacuum 4.6m deep (21.5psia) @22:19:00.36 Cartridge 57 filtering 517/1000ml at 0.66ml/s with 22.4psi vacuum @22:20:00.34 Cartridge 57 filtering 557/1000ml at 0.66ml/s with 29.5psi vacuum @22:21:00.35 Cartridge 57 filtering 596/1000ml at 0.66ml/s with 29.5psi vacuum @22:22:00.34 Cartridge 57 filtering 635/1000ml at 0.65ml/s with 24.4psi vacuum @22:23:00.35 Cartridge 57 filtering 674/1000ml at 0.65ml/s with 11.4psi vacuum 9.5m deep (28.8psia) @22:24:00.35 Cartridge 57 filtering 714/1000ml at 0.65ml/s with 21.2psi vacuum 4.3m deep (21.1psia) @22:25:00.34 Cartridge 57 filtering 753/1000ml at 0.65ml/s with 21.4psi vacuum @22:26:00.34 Cartridge 57 filtering 792/1000ml at 0.65ml/s with 25.9psi vacuum @22:27:00.34 Cartridge 57 filtering 831/1000ml at 0.65ml/s with 24.4psi vacuum @22:28:00.35 Cartridge 57 filtering 870/1000ml at 0.65ml/s with 17.5psi vacuum 8.3m deep (27.0psia) @22:29:00.34 Cartridge 57 filtering 909/1000ml at 0.65ml/s with 22.1psi vacuum @22:30:00.35 Cartridge 57 filtering 948/1000ml at 0.65ml/s with 15.0psi vacuum 5.2m deep (22.4psia) @22:31:00.35 Cartridge 57 filtering 987/1000ml at 0.65ml/s with 20.8psi vacuum @22:31:20.76 Abs.stop @22:31:20.90 Intake.close @22:31:20.97 Exhaust.close @22:31:21.21 Toroid.in :hold @22:31:21.27 Toroid.seek :bypass @22:31:22.90 Toroid.in :default @22:31:22.95 Toroid.seek :clear @22:31:24.57 Sampled 1000.0ml @22:31:25.03 GoodNews.email "After filtering 1000.0ml",Subject: "Processing microSPR_phyto cartridge in slot 57" @22:31:26.56 SPR.command "read_sequences" @22:31:26.96 SPR.command "stats_on" @22:31:27.12 SPR.command "start 0 1" @22:31:27.84 SPR.start /var/log/esp/spr24aug0122h.spr.bz2 @22:31:29.82 Handoff.select :waste @22:31:29.96 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:31:32.31 upsync @22:31:36.59 Syringe tare is 51% of full scale at 26C (drifted 0%) @22:31:36.62 PinPort.in :locking @22:31:36.67 PinPort.seek :locked @22:31:37.16 Archive.in :fast @22:31:37.25 Lysis2.in :fast @22:31:37.31 Lysis1.seek 1350uL @22:31:37.35 Archive.seek :full @22:31:37.53 Lysis2.seek 950uL @22:31:42.27 Plunge.seek :reagent @22:31:43.19 Evacuating puck with filter bubble point of 24psi @22:31:43.21 PinPort.seek :locked @22:31:43.29 Handoff.select :waste @22:31:43.33 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:31:49.92 Air syringe pumping up to 1 strokes to reach 8psi @22:31:49.94 Air stroke #1 of 1 @22:31:50.03 Air.in :pressurize @22:31:50.08 Air.seek :pressurized @22:31:55.70 1.3psi after pressurize @22:31:55.72 Air.in :compress @22:31:55.77 Air.seek :evac @22:31:58.80 3.7psi after compress @22:32:09.39 1.6psi after compress (stable) @22:32:09.40 Air.in :default @22:32:09.45 Air.hold @22:32:09.67 at 1.7psi Opened to waste @22:32:09.68 Handoff.select :blocked @22:32:09.82 Archive syringe pumping up to 8 strokes to reach 24psi @22:32:09.83 Archive stroke #1 of 8 @22:32:09.91 Archive.in :pressurize @22:32:09.96 Archive.seek :pressurized @22:32:22.45 2.5psi after pressurize @22:32:28.54 2.0psi after pressurize (stable) @22:32:28.56 Archive.in :compress @22:32:28.61 Archive.seek :empty @22:32:44.33 16.4psi after compress @22:32:54.92 15.5psi after compress (stable) @22:32:54.95 Archive.in :gulp @22:32:55.00 Archive.seek :topVent @22:33:01.30 16.5psi after gulp @22:33:06.89 15.8psi after gulp (stable) @22:33:06.90 Archive stroke #2 of 8 @22:33:06.99 Archive.in :pressurize @22:33:07.05 Archive.seek :pressurized @22:33:19.47 16.6psi after pressurize @22:33:25.56 15.8psi after pressurize (stable) @22:33:25.58 Archive.in :compress @22:33:25.63 Archive.seek :empty @22:33:34.12 Reached 24.1 of 24.0psi after pumping 3305uL (1.91 strokes) @22:33:34.18 Archive.in :default @22:33:34.28 Archive.hold @22:33:34.37 Handoff.select :waste @22:33:34.52 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:33:46.11 delay 1:00 @22:34:46.13 Handoff.select :blocked @22:34:46.30 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @22:35:08.89 Handoff.select :waste @22:35:09.01 0.9psi at evacEnd @22:35:09.02 Handoff.select :blocked @22:35:09.15 Archive.in :fast @22:35:09.20 Archive.seek :topVent @22:35:15.83 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @22:35:22.42 Stable @0.5psi after Handoff blocked @22:35:22.45 Archive syringe pumping up to 8 strokes to reach 24psi @22:35:22.46 Archive stroke #1 of 8 @22:35:22.54 Archive.in :pressurize @22:35:22.60 Archive.seek :pressurized @22:35:35.02 4.0psi after pressurize @22:35:35.04 Archive.in :compress @22:35:35.09 Archive.seek :empty @22:35:50.80 16.1psi after compress @22:36:01.39 14.7psi after compress (stable) @22:36:01.42 Archive.in :gulp @22:36:01.47 Archive.seek :topVent @22:36:07.76 15.9psi after gulp @22:36:13.86 15.1psi after gulp (stable) @22:36:13.88 Archive stroke #2 of 8 @22:36:13.96 Archive.in :pressurize @22:36:14.03 Archive.seek :pressurized @22:36:26.50 15.8psi after pressurize @22:36:26.53 Archive.in :compress @22:36:26.58 Archive.seek :empty @22:36:36.61 Reached 24.5 of 24.5psi after pumping 3338uL (1.93 strokes) @22:36:36.63 Archive.in :default @22:36:36.68 Archive.hold @22:36:36.78 Handoff.select :waste @22:36:36.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:36:44.03 delay 1:00 @22:37:44.05 Handoff.select :blocked @22:37:44.19 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @22:38:06.78 Handoff.select :waste @22:38:06.90 0.8psi at evacEnd @22:38:06.92 Air.seek :fullVent @22:38:08.24 Archive.in :fast @22:38:08.29 Archive.seek :topVent @22:38:15.01 Seawater evacuated, Pressure above/below filter released. @22:38:15.12 Lysis1.in :slow @22:38:15.21 Lysis1.seek 1050uL @22:38:45.41 delay 5 seconds @22:38:50.45 Handoff.select :blocked @22:38:50.65 Lysis1.seek 450uL @22:39:49.75 delay 3 seconds @22:39:52.84 Air.in mix with 1.57127 parts Lysis1 @22:39:53.59 Air.seek :evac @22:39:53.64 Lysis1.seek 300uL @22:40:08.58 Lysis1.seek :empty @22:40:38.30 Lysis buffer delivered @11.6psi @22:40:38.32 Polling Can every 2:00 @22:40:38.88 PuckHeater.configure :default #ambient 26.0C @22:40:38.97 PuckHeater.seek 79.6C @22:41:32.86 PuckHeater.hold 2:00,79.6C @22:43:32.18 Polling Can every 2:00 @22:43:32.73 PuckHeater.configure :default #ambient 26.0C @22:43:32.78 PuckHeater.hold 30 seconds,78.6C @22:44:02.80 Polling Can every 2:00 @22:44:03.35 PuckHeater.hold 30 seconds,78C @22:44:33.38 Polling Can every 2:00 @22:44:33.92 PuckHeater.configure :default #ambient 26.0C @22:44:33.98 PuckHeater.hold 10:00,77.5C @22:54:30.45 Lysis complete. Syringe pressure @13.4psi @22:54:30.48 Archive syringe pumping up to 5 strokes to reach 40psi @22:54:30.49 Archive stroke #1 of 5 @22:54:30.60 Archive.in :pressurize @22:54:30.65 Archive.seek :pressurized @22:54:43.07 13.8psi after pressurize @22:54:49.18 13.1psi after pressurize (stable) @22:54:49.23 Archive.in :compress @22:54:49.28 Archive.seek :empty @22:54:59.93 Reached 40.4 of 40.0psi after pumping 1621uL (0.94 strokes) @22:54:59.94 Archive.in :default @22:54:59.99 Archive.hold @22:55:00.07 delay 2 seconds @22:55:02.10 Archive.in :fast @22:55:02.17 Archive.seek :full @22:55:09.04 Lysis1.seek 138uL @22:55:22.75 delay 1 second @22:55:23.78 Archive.seek :full @22:55:23.86 Lysis1.seek :empty @22:55:37.60 Handoff.select :blocked @22:55:37.70 Initial air pressure @40.3psi @22:55:37.79 Lysis1.in :extract @22:55:37.87 Lysis1.seek 600uL,nil @22:55:42.60 Archive syringe pumping up to 6 strokes to reach 25psi @22:55:42.63 Archive stroke #1 of 6 @22:55:42.72 Archive.in :pressurize @22:55:42.78 Archive.seek :pressurized @22:55:43.36 Archive.in :default @22:55:43.42 Archive.hold @22:55:43.83 Initial 600uL lysate @14.2psi @22:55:43.84 Handoff.select :blocked @22:55:43.94 Initial air pressure @13.7psi @22:55:44.03 Lysis1.seek 825uL,nil @22:55:44.77 Archive syringe pumping up to 6 strokes to reach 17psi @22:55:44.79 Archive stroke #1 of 6 @22:55:45.05 Archive.in :pressurize @22:55:45.14 Archive.seek :pressurized @22:55:56.97 14.0psi after pressurize @22:56:02.78 Archive.in :default @22:56:02.85 Archive.hold @22:56:03.16 Recovered 825uL lysate @13.8psi @22:56:03.18 Air.in :halfSpeed @22:56:03.24 Air.seek :fullVent @22:56:05.25 Puck Top Vented, bottom @14.4psi @22:56:05.26 Handoff.select :waste @22:56:05.38 delay 5 seconds @22:56:10.46 Handoff open to waste, bottom @0.3psi @22:56:10.48 Air.in :default @22:56:10.57 Air.seek :evac @22:56:11.89 Lysis2.in :DAmix @22:56:12.00 Lysis1.in :DAmix @22:56:12.67 Lysis2.seek 700uL @22:56:12.71 Lysis1.seek 575uL @22:56:47.32 delay 5 seconds @22:56:52.34 Handoff.select SPR @22:56:52.49 SPR.command "start 2 14" @22:56:53.55 SPR stabilized @22:56:53.72 delay 10 seconds @22:57:03.74 SPR.command "start 2 0" @22:57:06.92 Waiting up to 11:00 for SPR to enter state 1 @22:59:04.60 SPR.command "hold_on" @22:59:05.99 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:59:06.70 Lysis2.seek 400uL @22:59:06.76 Lysis1.seek 275uL @22:59:48.24 delay 15 seconds @23:00:03.26 SPR.command "hold_off" @23:00:07.15 Waiting up to 1:00:00 for SPR to enter state 7 @23:16:23.15 SPRsummary:-38.5RIU,2.67e+09RIU,242RIU,3.25RIU,none,none,high after 2847s @23:30:40.07 SPR analysis completed @23:30:48.81 Ended log spr24aug0122h.spr.bz2 @23:30:48.82 SPR.command "stats_off" @23:30:49.12 Selecting Cartridge 56 @23:30:49.30 Gate.power :heat,:OFF @23:30:49.31 Gate.power :cartridge,:OFF @23:30:49.39 PinPort.in :unlocking @23:30:49.44 PinPort.seek :clear @23:30:49.92 Carousel.in :default @23:30:50.04 Carousel.home.seek 57 @23:30:50.38 Archive.in :unlocking @23:30:50.46 Archive.seek :clear @23:30:50.59 Air.in :unlocking @23:30:50.70 Air.seek :clear @23:30:50.81 Lysis2.in :unlocking @23:30:50.88 Lysis2.seek :clear @23:30:54.91 Lysis1.in :unlocking @23:30:55.03 Lysis1.seek :clear @23:30:55.13 Plunge.in :unlocking @23:30:55.22 Plunge.seek :clear @23:30:58.95 Carousel.seek 56 @23:30:59.50 Lysis1.in :fast @23:30:59.55 Lysis1.seek :locked @23:31:02.19 Plunge.in :default @23:31:02.30 Plunge.seek :locked @23:31:02.42 Air.in :fast @23:31:02.48 Air.seek :locked @23:31:03.46 Carousel.reconfigure :locked @23:31:03.61 Powering cartridge bus @23:31:06.29 Gate.setIR 1750,5000,0,6 @23:31:06.42 Gate.setIR 0,5000,0,6 @23:31:06.49 Gate.power :cartridge,:OFF @23:31:07.05 Mounted archive Cartridge @23:31:07.14 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 56" @23:31:07.45 Preparing to filter cartridge 56 @23:31:07.76 Priming sample loop w/100ml, bypass w/10ml @23:31:07.78 SamplePump.setPosition! 0ml @23:31:07.83 Bypass.open @23:31:09.26 Intake.open @23:31:10.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:31:12.69 Vacuum tare is 53% of full scale at 26C (drifted 2%) @23:31:12.94 Abs.in :default @23:31:13.04 Abs.hold @23:31:13.53 SamplePump.in :start @23:31:13.72 SamplePump.seek 10ml,1:10 @23:31:14.65 upsync @23:31:14.69 SamplePump.configure :prime @23:31:26.33 Abs.stop @23:31:26.39 SamplePump.setPosition! 0ml @23:31:26.44 Exhaust.open @23:31:27.79 Bypass.close @23:31:29.30 Abs.in :default @23:31:29.35 Abs.hold @23:31:29.66 SamplePump.in :start @23:31:29.73 SamplePump.seek 100ml,7:10 @23:31:30.36 SamplePump.configure :prime @23:33:32.00 Abs.stop @23:33:32.08 Bypass.open @23:33:32.76 Intake.close @23:33:33.50 Toroid.in :hold @23:33:33.56 Toroid.seek :crack @23:33:35.11 Toroid.seek :cartridge @23:33:36.22 Toroid.in :default @23:33:36.28 Toroid.seek :clear @23:33:38.34 Pressurizing cartridge to 26psi above 15.4psia @23:33:38.36 SamplePump.setPosition! 0ml @23:33:38.41 Intake.open @23:33:39.04 Bypass.close @23:33:39.71 Exhaust.close @23:33:40.45 Abs.in :default @23:33:40.51 Abs.hold @23:33:40.73 SamplePump.in :charge @23:33:40.79 SamplePump.seek 2.3ml,42 seconds @23:33:47.23 Abs.stop @23:33:47.29 Intake.close @23:33:47.99 Pressurized cartridge with 0.185ml to 26.3psi @23:33:48.01 Isolated @36.9psia (21.5psi) @23:33:48.02 Verifying cartridge holds at least 0.65..0.85 of 36.9psia @23:33:48.05 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:33:52.66 Cartridge pressure fell 1% to 36.5psia @23:33:52.75 Sampling 1000.0ml @23:33:52.76 SamplePump.setPosition! 0ml @23:33:52.82 Exhaust.open @23:33:53.43 Intake.open @23:33:54.76 Abs.in :default @23:33:54.90 Abs.hold @23:33:55.21 SamplePump.in :start @23:33:55.28 SamplePump.seek 1000ml,1:23:50 @23:33:55.90 SamplePump.configure :sample @23:33:58.31 Cartridge 56 filtering 2/1000ml at 0.79ml/s with 12.7psi vacuum 12.3m deep (32.8psia) @23:34:58.37 Cartridge 56 filtering 41/1000ml at 0.65ml/s with 24.4psi vacuum @23:35:58.36 Cartridge 56 filtering 79/1000ml at 0.64ml/s with 21.7psi vacuum @23:36:58.37 Cartridge 56 filtering 118/1000ml at 0.64ml/s with 25.8psi vacuum @23:37:58.35 Cartridge 56 filtering 156/1000ml at 0.64ml/s with 22.8psi vacuum @23:38:58.36 Cartridge 56 filtering 194/1000ml at 0.63ml/s with 17.7psi vacuum 4.2m deep (20.8psia) @23:39:58.38 Cartridge 56 filtering 232/1000ml at 0.63ml/s with 28.8psi vacuum @23:40:58.36 Cartridge 56 filtering 270/1000ml at 0.63ml/s with 20.5psi vacuum @23:41:58.36 Cartridge 56 filtering 308/1000ml at 0.63ml/s with 17.6psi vacuum @23:42:58.37 Cartridge 56 filtering 346/1000ml at 0.63ml/s with 31.8psi vacuum @23:43:58.36 Cartridge 56 filtering 383/1000ml at 0.63ml/s with 20.0psi vacuum @23:44:58.38 Cartridge 56 filtering 421/1000ml at 0.63ml/s with 22.1psi vacuum @23:45:58.37 Cartridge 56 filtering 459/1000ml at 0.63ml/s with 13.2psi vacuum 13.4m deep (34.5psia) @23:46:58.37 Cartridge 56 filtering 496/1000ml at 0.62ml/s with 21.9psi vacuum @23:47:58.38 Cartridge 56 filtering 534/1000ml at 0.62ml/s with 21.7psi vacuum 5.2m deep (22.3psia) @23:48:58.36 Cartridge 56 filtering 571/1000ml at 0.62ml/s with 27.5psi vacuum @23:49:58.36 Cartridge 56 filtering 608/1000ml at 0.62ml/s with 21.8psi vacuum @23:50:58.36 Cartridge 56 filtering 646/1000ml at 0.62ml/s with 18.3psi vacuum @23:51:58.37 Cartridge 56 filtering 683/1000ml at 0.62ml/s with 12.8psi vacuum 12.6m deep (33.4psia) @23:52:58.36 Cartridge 56 filtering 720/1000ml at 0.62ml/s with 35.2psi vacuum @23:53:58.37 Cartridge 56 filtering 757/1000ml at 0.62ml/s with 36.0psi vacuum @23:54:58.36 Cartridge 56 filtering 795/1000ml at 0.62ml/s with 16.2psi vacuum 6.4m deep (24.2psia) @23:55:58.38 Cartridge 56 filtering 832/1000ml at 0.62ml/s with 30.6psi vacuum @23:56:58.36 Cartridge 56 filtering 869/1000ml at 0.62ml/s with 28.8psi vacuum @23:57:58.36 Cartridge 56 filtering 907/1000ml at 0.62ml/s with 9.5psi vacuum 8.2m deep (26.8psia) @23:58:58.37 Cartridge 56 filtering 944/1000ml at 0.62ml/s with 13.4psi vacuum 5.7m deep (23.1psia) @23:59:58.36 Cartridge 56 filtering 981/1000ml at 0.62ml/s with 34.8psi vacuum @00:00:28.81PDT02-Aug-24 Abs.stop @00:00:29.52 Intake.close @00:00:29.60 Exhaust.close @00:00:29.70 Handoff.select :close @00:00:29.92 Toroid.in :hold @00:00:29.98 Toroid.seek :bypass @00:00:31.61 Toroid.in :default @00:00:31.67 Toroid.seek :clear @00:00:33.28 Sampled 1000.0ml @00:00:33.71 GoodNews.email "After filtering 1000.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 56" @00:00:34.22 Plunge.seek :reagent @00:00:35.06 Sample loop closed. Ready to Deliver Reagents @00:00:35.08 Archive.in :default @00:00:35.13 Archive.seek :sealed @00:00:39.07 Archive.seek :evac @00:00:41.17 upsync @00:00:53.32 delay 1:00 @00:01:53.36 Archive.seek :clear @00:02:11.05 RNAlater soak for 10:00 @00:12:11.09 Archive.seek :tVent @00:12:13.62 delay 1 second @00:12:14.65 Archive.seek :evac @00:12:30.29 delay 10 seconds @00:12:40.33 Archive.seek :tVent @00:12:55.97 delay 1 second @00:12:57.00 Archive.seek :evac @00:13:12.63 delay 10 seconds @00:13:22.67 Sample preserved and evacuated @00:13:22.94 Carousel.in :default @00:13:23.06 Carousel.home.seek 56 @00:13:23.38 Plunge.seek :locked @00:13:24.29 Archive.in :unlocking @00:13:24.40 Archive.seek :clear @00:13:24.52 Air.in :unlocking @00:13:24.66 Air.seek :clear @00:13:24.78 Lysis1.in :unlocking @00:13:25.39 Lysis1.seek :clear @00:13:29.01 Gate.power :analytic1,:OFF @00:13:29.03 Gate.power :motor,:OFF @00:13:29.04 Gate.power :core,:OFF @00:13:29.05 Gate.power :main,:OFF @00:13:29.07 Safely stopped and ready to power off @05:29:46.93 Session Begun @05:29:50.13 -> slot {[50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000.0, lysed: 825, spr: spr24aug0122h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0120h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0119h.spr.bz2}} @05:31:22.64 -> Cartrige.release 51..6 @05:31:22.67 NameError in bill -- uninitialized constant Cartrige @05:31:35.45 -> Cartridge.release 51..6 46 @05:31:39.28 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [50,48,46,44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [51,49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 55..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000.0, lysed: 825, spr: spr24aug0122h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0120h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50.0, lysed: 825, spr: spr24aug0119h.spr.bz2}} @05:32:18.77 -> PhytoSmpler.xray @05:32:18.80 NameError in bill -- uninitialized constant PhytoSmpler @05:32:26.13 -> PhytoSampler.xray @05:33:01.14 -> Cmd.stop @05:33:01.20 ready to power off :STOPPED @05:33:05.83 -> l :l @05:33:11.70 -> exit @05:33:11.73 Session Ended @09:01:18.86PDT02-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @09:01:22.65 >BREAK< ESP3 Gateway v1.43 for core bus @09:01:22.68 >BREAK< ESP3 Gateway v1.43 for cartridge bus @09:01:26.48 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @09:01:27.22 Sea pressure (Abs) tare is 6% of full scale @09:01:40.28 Polling Can every 2:00 @09:01:40.35 Waiting for espclients... @09:02:19.40 Session Begun @09:02:42.66 -> ESP.ready! @09:02:42.71 Gate.power :sampler,:OFF,limit to 3500mA @09:02:42.74 Gate.power :skimmer,:OFF,limit to 3500mA @09:02:42.78 Gate.power :main,:ON,limit to 5000mA @09:02:42.81 Gate.power :analytic1,:ON,limit to 4000mA @09:02:43.01 Powering core bus @09:02:45.23 SPR Module 1, FW Rev. B, FPGA Rev. C @09:02:45.79 Plunge.configure :default @09:02:45.90 SamplePump.configure :default @09:02:46.00 Toroid.configure :default @09:02:46.11 Carousel.configure :default @09:02:46.21 Abs.configure :default @09:02:46.33 PhaseSeparator.configure :default @09:02:46.44 Archive.configure :default @09:02:46.55 Air.configure :default @09:02:46.63 Lysis1.configure :default @09:02:46.71 Lysis2.configure :default @09:02:46.82 PinPort.configure :default @09:02:46.90 All elf controllers running firmware version 4.92 @09:02:46.94 Gate.power :motor,:ON,limit to 5000mA @09:02:47.24 Plunge.home.jog 0 @09:02:48.05 PinPort.home.jog 0 @09:02:48.10 Lysis1.home.jog 0 @09:02:48.14 Archive.home.jog 0 @09:02:48.18 Toroid.home.jog 0 @09:02:48.22 Lysis2.home.jog 0 @09:02:48.27 Air.home.jog 0 @09:02:48.31 Carousel.home.jog 0 @09:02:49.69 Plunge.in :homing @09:02:49.90 Plunge.jog 1594 @09:02:49.97 PinPort.in :homing @09:02:50.02 Lysis1.in :homing @09:02:50.06 Archive.in :homing @09:02:50.11 Lysis2.in :homing @09:02:50.17 Air.in :homing @09:02:50.23 Toroid.in :homing @09:02:50.56 PinPort.jog -108 @09:02:50.61 Lysis1.jog 1395 @09:02:50.71 Archive.jog 1392 @09:02:50.76 Lysis2.jog 1399 @09:02:50.85 Air.jog 1356 @09:02:50.90 Toroid.jog 624 @09:02:51.31 PinPort.setRawPosition! 0 @09:02:51.38 PinPort.seek :home @09:02:51.49 PinPort.in :default @09:02:51.60 Plunge.setRawPosition! 50 @09:02:51.68 Plunge.seek :home @09:02:51.99 Plunge.in :default @09:02:52.13 Lysis1.setRawPosition! 50 @09:02:52.22 Lysis1.seek :home @09:02:52.38 Lysis2.setRawPosition! 50 @09:02:52.41 Air.setRawPosition! 50 @09:02:52.49 Archive.setRawPosition! 50 @09:02:52.57 Lysis2.seek :home @09:02:52.65 Air.seek :home @09:02:52.81 Archive.seek :home @09:02:53.03 Lysis1.in :default @09:02:53.23 Lysis2.in :default @09:02:53.26 Air.in :default @09:02:53.29 Archive.in :default @09:02:53.49 Toroid.setRawPosition! 0 @09:02:53.57 Toroid.seek :home @09:02:53.70 Toroid.in :default @09:02:53.83 Toroid.seek :clear @09:02:55.52 Carousel.in :default @09:02:55.67 Carousel.home.seek 56 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 56 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.19psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @09:03:00.66 -> can Can@09:02:58, 21.7C, 50% humidity, 14.2psia, 14.836V, 0.513A, 0.009Ah, 7.62W @09:03:28.79 -> exit @09:03:28.82 Session Ended @09:04:56.82 Session Begun @09:05:03.29 -> power :power @09:05:45.52 -> ESP.shutdown @09:05:45.73 Gate.power :analytic1,:OFF @09:05:45.76 Gate.power :motor,:OFF @09:05:45.79 Gate.power :core,:OFF @09:05:45.83 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @09:05:55.61 -> exit @09:05:55.62 Session Ended @06:23:28.85PDT05-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @06:23:33.37 >BREAK< ESP3 Gateway v1.43 for core bus @06:23:33.40 >BREAK< ESP3 Gateway v1.43 for cartridge bus @06:23:37.59 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @06:23:38.31 Sea pressure (Abs) tare is 6% of full scale @06:23:52.54 Polling Can every 2:00 @06:23:52.72 Waiting for espclients... @06:23:53.75 Session Begun @06:23:56.94 -> ESP.ready! @06:23:57.01 Gate.power :sampler,:OFF,limit to 3500mA @06:23:57.03 Gate.power :skimmer,:OFF,limit to 3500mA @06:23:57.06 Gate.power :main,:ON,limit to 5000mA @06:23:57.10 Gate.power :analytic1,:ON,limit to 4000mA @06:23:57.34 Powering core bus @06:23:59.54 SPR Module 1, FW Rev. B, FPGA Rev. C @06:24:00.12 Plunge.configure :default @06:24:00.24 SamplePump.configure :default @06:24:00.35 Toroid.configure :default @06:24:00.51 Carousel.configure :default @06:24:00.63 Abs.configure :default @06:24:00.78 PhaseSeparator.configure :default @06:24:00.88 Archive.configure :default @06:24:00.98 Air.configure :default @06:24:01.08 Lysis1.configure :default @06:24:01.16 Lysis2.configure :default @06:24:01.25 PinPort.configure :default @06:24:01.35 All elf controllers running firmware version 4.92 @06:24:01.40 Gate.power :motor,:ON,limit to 5000mA @06:24:02.15 Plunge.home.jog 0 @06:24:02.23 Toroid.home.jog 0 @06:24:02.43 Carousel.home.jog 0 @06:24:02.58 Archive.home.jog 0 @06:24:02.80 Air.home.jog 0 @06:24:02.92 Lysis1.home.jog 0 @06:24:03.08 Lysis2.home.jog 0 @06:24:03.20 PinPort.home.jog 0 @06:24:04.74 Air.in :homing @06:24:04.97 Plunge.in :homing @06:24:05.01 Lysis1.in :homing @06:24:05.04 Toroid.in :homing @06:24:05.14 Archive.in :homing @06:24:05.31 Air.jog 1366 @06:24:05.42 Plunge.jog 158 @06:24:05.46 Lysis1.jog 1398 @06:24:05.53 Toroid.jog 619 @06:24:05.64 Archive.jog 1397 @06:24:06.00 PinPort.in :homing @06:24:06.06 Lysis2.in :homing @06:24:06.30 PinPort.jog -108 @06:24:06.33 Lysis2.jog 1404 @06:24:06.53 Plunge.setRawPosition! 50 @06:24:06.60 Plunge.seek :home @06:24:06.81 PinPort.setRawPosition! 0 @06:24:06.93 Lysis1.setRawPosition! 50 @06:24:06.98 Air.setRawPosition! 50 @06:24:07.04 PinPort.seek :home @06:24:07.34 Lysis1.seek :home @06:24:07.38 Air.seek :home @06:24:07.69 PinPort.in :default @06:24:07.73 Lysis2.setRawPosition! 50 @06:24:07.77 Plunge.in :default @06:24:07.81 Archive.setRawPosition! 50 @06:24:08.01 Lysis2.seek :home @06:24:08.09 Archive.seek :home @06:24:08.31 Lysis1.in :default @06:24:08.39 Air.in :default @06:24:08.43 Toroid.setRawPosition! 0 @06:24:08.65 Toroid.seek :home @06:24:08.83 Archive.in :default @06:24:08.86 Toroid.in :default @06:24:08.89 Lysis2.in :default @06:24:09.36 Toroid.seek :clear @06:24:11.11 Carousel.in :default @06:24:11.25 Carousel.home.seek 59 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 59 Abs = Absolute Pressure (Leak) Sensor @14psia PhaseSeparator = Intake Head Pressure @0.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @06:24:23.20 -> SC.to 1 @06:24:23.23 Carousel.seek 1 Storage Carousel (SC) at 1 @06:24:41.20 -> SC.to 60 @06:24:41.25 Carousel.seek 60 Storage Carousel (SC) at 60 @06:25:10.03 -> Power.sampler :ON @06:25:10.05 Gate.power :sampler,:ON true @06:25:30.48 -> Power.sampler :OFF @06:25:30.50 Gate.power :sampler,:OFF false @06:26:04.88 -> Power.sampler :ON @06:26:04.93 Gate.power :sampler,:ON true @06:26:36.33 -> require 'vacuumCart' true @06:26:41.87 -> refillSampleLineas :refillSampleLineas @06:27:12.42 -> refillSampleLines @06:27:12.47 Refilling sample & bypass lines with fluid. @06:27:13.75 Toroid.seek :clear @06:27:14.04 Carousel.in :default @06:27:14.29 Carousel.home.seek 60 @06:27:14.72 Waiting for nadia to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:27:15.89 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:27:15.91 Bypass.open @06:27:16.68 Intake.open @06:27:18.06 SamplePump.setPosition! 0ml @06:27:18.22 SamplePump.seek 30ml @06:27:55.47 Bypass.close @06:27:56.15 Exhaust.open @06:27:56.91 SamplePump.seek 55ml @06:28:28.06 delay 1 second @06:28:29.09 Intake.close @06:28:29.17 Exhaust.close @06:28:29.97 Sampling Loop filled with water @06:28:32.00 -> Power.sampler :off @06:28:32.03 Gate.power :sampler,:OFF false @06:29:06.26 -> Power.skimmer :on;delay 15;refillSampleLines @06:29:06.29 Gate.power :skimmer,:ON @06:29:06.36 delay 15 seconds @06:29:21.42 Refilling sample & bypass lines with fluid. @06:29:22.37 Toroid.seek :clear @06:29:22.65 Carousel.in :default @06:29:22.84 Carousel.home.seek 60 @06:29:23.17 Waiting for nadia to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @06:31:19.52 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @06:31:19.54 Bypass.open @06:31:20.23 Intake.open @06:31:20.93 SamplePump.setPosition! 0ml @06:31:21.07 SamplePump.seek 30ml @06:31:58.30 Bypass.close @06:31:58.99 Exhaust.open @06:31:59.72 SamplePump.seek 55ml @06:32:27.20 SamplePump.hold @06:32:27.41 delay 1 second @06:32:28.45 Intake.close @06:32:28.55 Exhaust.close @06:32:29.42 UserAbort in nadia -- Sample Pump (SP) seek to 55ml Aborted! @06:32:41.12 -> BACsampler.prime 100 @06:32:41.22 Priming sample loop w/100ml, bypass w/50ml @06:32:41.24 SamplePump.setPosition! 0ml @06:32:41.33 Bypass.open @06:32:42.78 Intake.open @06:32:43.57 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:32:46.20 Vacuum tare is 51% of full scale at 21C (drifted 0%) @06:32:46.41 Abs.in :default @06:32:46.48 Abs.hold @06:32:46.59 PhaseSeparator.in :full @06:32:46.70 PhaseSeparator.hold @06:32:46.94 Gate.power :skimmer,:OFF @06:32:46.99 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:32:47.27 SamplePump.stop @06:32:47.43 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.23psi @06:32:47.46 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:32:47.52 PhaseSeparator.in :filling @06:32:47.61 PhaseSeparator.hold @06:32:47.72 Gate.power :sampler,:ON @06:32:56.31 Head pressure reached 1.14psi in 9 seconds @06:32:56.34 Abs.hold @06:32:56.46 PhaseSeparator.in :full @06:32:56.54 PhaseSeparator.hold @06:32:57.37 Waiting for sampler to finish priming... @06:32:57.39 delayUntil "06:34:17.75" @06:33:01.68 Intake.close @06:33:01.80 Bypass.close @06:33:02.61 Abs.stop @06:33:02.73 PhaseSeparator.stop @06:33:02.82 UserAbort in nadia -- User Abort!! @06:33:12.72 -> Power.skimmer :off @06:33:12.74 Gate.power :skimmer,:OFF false @06:33:21.58 -> BACsampler.prime @06:33:21.69 Priming sample loop w/150ml, bypass w/50ml @06:33:21.71 SamplePump.setPosition! 0ml @06:33:21.79 Bypass.open @06:33:23.28 Intake.open @06:33:24.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:33:26.71 Vacuum tare is 51% of full scale at 21C (drifted -0%) @06:33:26.90 Abs.in :default @06:33:26.98 Abs.hold @06:33:27.07 PhaseSeparator.hold @06:33:27.33 Waiting for sampler to finish priming... @06:33:27.35 delayUntil "06:34:17.75" @06:33:35.29 Intake.close @06:33:35.34 Bypass.close @06:33:36.34 Abs.stop @06:33:36.45 PhaseSeparator.stop @06:33:36.55 UserAbort in nadia -- User Abort!! @06:33:50.98 -> Power.sampler :off @06:33:51.00 Gate.power :sampler,:OFF false @06:40:16.98 -> BACsampler.prime;Power.sampler :off @06:40:17.10 Priming sample loop w/150ml, bypass w/50ml @06:40:17.12 SamplePump.setPosition! 0ml @06:40:17.20 Bypass.open @06:40:18.68 Intake.open @06:40:19.46 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:40:22.09 Vacuum tare is 51% of full scale at 22C (drifted -0%) @06:40:22.30 Abs.in :default @06:40:22.39 Abs.hold @06:40:22.52 PhaseSeparator.hold @06:40:22.84 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:40:23.01 SamplePump.stop @06:40:23.16 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.12psi @06:40:23.20 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:40:23.27 PhaseSeparator.in :filling @06:40:23.35 PhaseSeparator.hold @06:40:23.47 Gate.power :sampler,:ON @06:40:31.51 Head pressure reached 1.29psi in 9 seconds @06:40:31.53 Abs.hold @06:40:31.66 PhaseSeparator.in :full @06:40:31.74 PhaseSeparator.hold @06:40:31.99 Waiting for sampler to finish priming... @06:40:32.00 delayUntil "06:41:53.49" @06:41:53.56 SamplePump.in :start @06:41:53.67 SamplePump.seek 50ml,3:50 @06:41:54.41 SamplePump.configure :prime @06:42:55.09 Abs.stop @06:42:55.18 PhaseSeparator.stop @06:42:55.25 SamplePump.setPosition! 0ml @06:42:55.32 Exhaust.open @06:42:56.69 Bypass.close @06:42:58.22 Abs.in :default @06:42:58.31 Abs.hold @06:42:58.41 PhaseSeparator.hold @06:42:58.80 SamplePump.in :start @06:42:58.89 SamplePump.seek 150ml,10:30 @06:42:59.49 SamplePump.configure :prime @06:45:58.08 SamplePump.stop @06:45:58.24 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.27psi @06:45:58.28 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:45:58.33 PhaseSeparator.in :filling @06:45:58.40 PhaseSeparator.hold @06:45:58.51 Gate.power :sampler,:OFF @06:45:58.91 Gate.power :sampler,:ON @06:46:10.45 PhaseSeparator.stop @06:46:10.56 Abs.stop @06:46:10.69 UserAbort in nadia -- User Abort!! @06:47:23.05 -> Power.sampler :off @06:47:23.06 Gate.power :sampler,:OFF false @06:52:53.70 -> BACsampler.source=skimmer :skimmer @06:52:59.76 -> BACsampler.prime @06:52:59.87 Priming sample loop w/150ml, bypass w/50ml @06:52:59.90 SamplePump.setPosition! 0ml @06:52:59.98 Exhaust.close @06:53:00.61 Bypass.open @06:53:02.19 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @06:53:04.81 Vacuum tare is 51% of full scale at 23C (drifted -0%) @06:53:04.99 Abs.in :default @06:53:05.07 Abs.hold @06:53:05.17 PhaseSeparator.in :full @06:53:05.26 PhaseSeparator.hold @06:53:05.52 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @06:53:05.67 SamplePump.stop @06:53:05.81 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.11psi @06:53:05.84 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:53:05.92 PhaseSeparator.in :filling @06:53:05.99 PhaseSeparator.hold @06:53:06.11 Gate.power :skimmer,:ON @06:53:15.19 Head pressure reached 1.41psi in 10 seconds @06:53:15.21 Abs.hold @06:53:15.34 PhaseSeparator.in :full @06:53:15.42 PhaseSeparator.hold @06:53:15.67 Waiting for skimmer to finish priming... @06:53:15.69 delayUntil "06:54:36.14" @06:54:36.21 SamplePump.in :start @06:54:36.30 SamplePump.seek 50ml,3:50 @06:54:36.90 SamplePump.configure :prime @06:55:37.70 Abs.stop @06:55:37.78 PhaseSeparator.stop @06:55:37.85 SamplePump.setPosition! 0ml @06:55:37.91 Exhaust.open @06:55:39.26 Bypass.close @06:55:40.82 Abs.in :default @06:55:40.89 Abs.hold @06:55:41.60 PhaseSeparator.hold @06:55:41.98 SamplePump.in :start @06:55:42.08 SamplePump.seek 150ml,10:30 @06:55:42.68 SamplePump.configure :prime @06:58:01.34 SamplePump.stop @06:58:01.56 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.07psi @06:58:01.60 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @06:58:01.70 PhaseSeparator.in :filling @06:58:01.78 PhaseSeparator.hold @06:58:01.90 Gate.power :skimmer,:OFF @06:58:02.28 Gate.power :skimmer,:ON @06:58:17.31 Gate.power :skimmer,:OFF @06:58:17.69 Gate.power :skimmer,:ON @06:58:22.79 Head pressure reached 1.42psi in 22 seconds @06:58:22.81 Abs.hold @06:58:22.93 PhaseSeparator.in :full @06:58:23.00 PhaseSeparator.hold @06:58:23.35 SamplePump.in :start @06:58:23.44 SamplePump.seek 150ml,2:55 @06:58:24.04 SamplePump.configure :prime @06:59:07.84 Abs.stop @06:59:07.92 PhaseSeparator.stop BACsampler @07:00:00.98 -> Power.skimmer :off @07:00:01.00 Gate.power :skimmer,:OFF false @07:20:00.62 -> Power.sampler :On @07:20:00.67 Gate.power :sampler,:ON true @07:20:16.45 -> Power.sampler :Off @07:20:16.48 Gate.power :sampler,:OFF false @07:20:47.97 -> Power.skimmer :on @07:20:48.00 Gate.power :skimmer,:ON true @07:21:05.18 -> Power.skimmer :off @07:21:05.19 Gate.power :skimmer,:OFF false @07:46:23.25 Session Ended @08:04:24.44 Slide::Error in core -- Storage Carousel (SC) positionErr at 60 (sensed end - 8 counts) Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @08:45:10.11 Session Begun @08:45:15.74 -> ESP.ready! @08:45:16.56 Carousel.in :default @08:45:16.66 Carousel.home.jog 0 @08:45:17.17 Toroid.seek :clear @08:45:17.37 Carousel.in :default @08:45:17.51 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 150ml Intake is opened Exhaust is opened Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.16psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @08:45:20.69 -> can Can@08:45:20, 24.1C, 46% humidity, 14.4psia, 14.846V, 0.487A, 1.474Ah, 7.22W @08:46:06.85 -> BACsampler.source :skimmer @08:46:22.08 -> BACsampler.source = sampler :sampler @08:46:35.26 -> BACsampler.prime @08:46:35.36 Priming sample loop w/150ml, bypass w/50ml @08:46:35.38 SamplePump.setPosition! 0ml @08:46:35.47 Exhaust.close @08:46:36.11 Bypass.open @08:46:37.77 Abs.in :default @08:46:37.84 Abs.hold @08:46:37.95 PhaseSeparator.hold @08:46:38.76 SamplePump.stop @08:46:38.91 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.15psi @08:46:38.94 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @08:46:39.01 PhaseSeparator.in :filling @08:46:39.09 PhaseSeparator.hold @08:46:39.22 Gate.power :sampler,:ON @08:46:46.21 Head pressure reached 2.15psi in 8 seconds @08:46:46.24 Abs.hold @08:46:46.36 PhaseSeparator.in :full @08:46:46.44 PhaseSeparator.hold @08:46:46.73 Waiting for sampler to finish priming... @08:46:46.75 delayUntil "08:48:09.24" @08:48:09.31 SamplePump.in :start @08:48:09.41 SamplePump.seek 50ml,3:50 @08:48:10.00 SamplePump.configure :prime @08:49:10.86 Abs.stop @08:49:10.95 PhaseSeparator.stop @08:49:11.03 SamplePump.setPosition! 0ml @08:49:11.12 Exhaust.open @08:49:12.50 Bypass.close @08:49:14.08 Abs.in :default @08:49:14.15 Abs.hold @08:49:14.26 PhaseSeparator.hold @08:49:14.70 SamplePump.in :start @08:49:14.80 SamplePump.seek 150ml,10:30 @08:49:15.35 SamplePump.configure :prime @08:52:18.40 Abs.stop @08:52:18.49 PhaseSeparator.stop BACsampler @08:58:51.83 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @10:15:08.26 Session Begun @10:15:14.90 -> can Can@10:15:08, 24.8C, 45% humidity, 14.4psia, 14.805V, 0.990A, 3.127Ah, 14.65W @10:23:43.14 -> exit @10:23:43.17 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @10:47:09.70 Session Begun @10:47:23.68 -> ESP.ready! @10:47:24.50 Toroid.seek :clear @10:47:24.73 Carousel.in :default @10:47:24.88 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 150ml Intake is opened Exhaust is opened Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.04psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:47:59.41 -> deep: fire lysis @10:47:59.44 SyntaxError in ben -- compile error ben:2: syntax error, unexpected tLABEL deep: fire lysis ^ @10:48:38.73 -> deep; fire lysis # @10:48:40.29 Toroid.seek :clear @10:48:40.55 Carousel.in :default @10:48:40.73 Carousel.home.seek 60 @10:48:41.02 Selecting Cartridge 50 @10:48:41.34 Carousel.seek 50 @10:48:43.73 Lysis1.in :fast @10:48:43.84 Lysis1.seek :locked @10:48:46.48 Plunge.seek :locked @10:48:46.71 Air.in :fast @10:48:46.80 Air.seek :locked @10:48:47.80 Carousel.reconfigure :locked @10:48:47.98 Powering cartridge bus @10:48:50.67 Gate.setIR 1750,5000,0,6 @10:48:50.82 Gate.setIR 0,5000,0,6 @10:48:50.91 PuckHeater.configure :default @10:48:51.01 Gate.power :heat,:ON,limit to 5000mA @10:48:51.77 Mounted lysis Cartridge board#Q05053 @10:48:51.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:48:51.89 PhytoSampler with {source: :sampler} @10:48:51.93 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 50" @10:48:52.33 Preparing to filter cartridge 50 @10:48:52.60 Gate.power :sampler,:OFF @10:48:52.71 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @10:48:58.37 upsync @10:49:25.33 Intake Head Pressure tare is 4% of full scale at 25C (drifted 3%) @10:49:25.35 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @10:49:25.42 PhaseSeparator.in :filling @10:49:25.49 PhaseSeparator.hold @10:49:25.60 Gate.power :sampler,:ON @10:49:31.81 Head pressure reached 1.89psi in 7 seconds @10:49:31.85 Air.in :default @10:49:31.92 Bypass.open @10:49:32.06 Air.seek :sealed @10:49:32.71 Intake.close @10:49:33.52 Toroid.in :hold @10:49:33.60 Toroid.seek :crack @10:49:35.17 Toroid.seek :cartridge @10:49:36.84 Toroid.in :default @10:49:36.94 Toroid.seek :clear @10:49:38.85 Intake.open @10:49:39.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:49:42.27 Vacuum tare is 51% of full scale at 25C (drifted -0%) @10:49:42.46 Pressurizing cartridge to 26psi above 14.9psia @10:49:42.48 SamplePump.setPosition! 0ml @10:49:42.57 Bypass.close @10:49:43.27 Exhaust.close @10:49:44.08 Abs.in :default @10:49:44.17 Abs.hold @10:49:44.27 PhaseSeparator.in :full @10:49:44.36 PhaseSeparator.hold @10:49:44.62 Waiting for sampler to finish priming... @10:49:44.66 delayUntil "10:50:55.63" @10:50:55.70 SamplePump.in :charge @10:50:55.80 SamplePump.seek 2.3ml,42 seconds @10:51:08.83 Abs.stop @10:51:08.92 PhaseSeparator.stop @10:51:09.01 Intake.close @10:51:09.81 Pressurized cartridge with 0.761ml to 26.3psi @10:51:09.83 Isolated @36.7psia (21.8psi) @10:51:09.87 Verifying cartridge holds at least 0.65..0.85 of 36.7psia @10:51:09.93 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:51:14.55 Cartridge pressure fell 1% to 36.5psia @10:51:14.72 Sampling 25.0ml @10:51:14.74 SamplePump.setPosition! 0ml @10:51:14.85 Exhaust.open @10:51:15.53 Intake.open @10:51:16.46 Abs.in :default @10:51:16.55 Abs.hold @10:51:16.66 PhaseSeparator.hold @10:51:17.06 SamplePump.in :start @10:51:17.17 SamplePump.seek 25ml,2:35 @10:51:17.75 SamplePump.configure :sample @10:51:20.16 Cartridge 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum @10:51:58.29 Abs.stop @10:51:58.37 PhaseSeparator.stop @10:51:58.54 Intake.close @10:51:58.67 Exhaust.close @10:51:59.55 Toroid.in :hold @10:51:59.65 Toroid.seek :bypass @10:52:01.32 Toroid.in :default @10:52:01.43 Toroid.seek :clear @10:52:03.07 Sampled 25.0ml @10:52:03.57 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 50" @10:52:05.19 SPR.command "read_sequences" @10:52:05.30 SPR.command "stats_on" @10:52:05.50 SPR.command "start 0 1" @10:52:06.18 SPR.start /var/log/esp/spr24aug0510h.spr.bz2 @10:52:06.24 Gate.power :sampler,:OFF @10:52:08.10 Handoff.select :waste @10:52:08.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:52:08.74 upsync @10:52:14.94 Syringe tare is 51% of full scale at 25C (drifted 0%) @10:52:14.97 PinPort.in :locking @10:52:15.08 PinPort.seek :locked @10:52:15.63 Archive.in :fast @10:52:15.70 Lysis2.in :fast @10:52:15.81 Lysis1.seek 1350uL @10:52:15.96 Archive.seek :full @10:52:16.00 Lysis2.seek 950uL @10:52:21.00 Plunge.seek :reagent @10:52:21.94 Evacuating puck with filter bubble point of 24psi @10:52:21.97 PinPort.seek :locked @10:52:22.60 Handoff.select :waste @10:52:22.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:52:29.41 Air syringe pumping up to 1 strokes to reach 8psi @10:52:29.43 Air stroke #1 of 1 @10:52:29.58 Air.in :pressurize @10:52:29.67 Air.seek :pressurized @10:52:35.32 1.1psi after pressurize @10:52:35.35 Air.in :compress @10:52:35.46 Air.seek :evac @10:52:38.50 1.3psi after compress @10:52:49.09 0.0psi after compress (stable) @10:52:49.11 Air.in :default @10:52:49.27 Air.hold @10:52:49.56 at -0.0psi Opened to waste @10:52:49.58 Handoff.select :blocked @10:52:49.76 Archive syringe pumping up to 8 strokes to reach 24psi @10:52:49.77 Archive stroke #1 of 8 @10:52:49.90 Archive.in :pressurize @10:52:49.99 Archive.seek :pressurized @10:53:02.48 10.7psi after pressurize @10:53:08.10 10.0psi after pressurize (stable) @10:53:08.21 Archive.in :compress @10:53:08.30 Archive.seek :empty @10:53:24.04 21.7psi after compress @10:53:29.26 -> cube@10.1.20.12's password: @10:53:29.30 SyntaxError in ben -- compile error ben:4: `@1' is not allowed as an instance variable name @10:53:42.63 23.3psi after compress (stable) @10:53:42.70 Archive.in :gulp @10:53:42.81 Archive.seek :topVent @10:53:43.05 Reached 23.9 of 24.0psi after pumping 1730uL (1.00 strokes) @10:53:43.08 Archive.in :default @10:53:43.23 Archive.hold @10:53:43.35 Handoff.select :waste @10:53:43.51 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:53:57.62 delay 1:00 @10:54:57.64 Handoff.select :blocked @10:54:57.84 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @10:54:58.63 -> 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum @10:54:58.70 SyntaxError in ben -- compile error ben:5: syntax error, unexpected tIDENTIFIER, expecting $end 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum ^ @10:54:58.89 -> @10:51:58.29 Abs.stop @10:54:58.93 SyntaxError in ben -- compile error ben:6: `@1' is not allowed as an instance variable name @10:54:59.12 -> @10:51:58.37 PhaseSeparator.stop @10:54:59.17 SyntaxError in ben -- compile error ben:7: `@1' is not allowed as an instance variable name @10:54:59.33 -> @10:51:58.54 Intake.close @10:54:59.36 SyntaxError in ben -- compile error ben:8: `@1' is not allowed as an instance variable name @10:54:59.59 -> @10:51:58.67 Exhaust.close @10:54:59.64 SyntaxError in ben -- compile error ben:9: `@1' is not allowed as an instance variable name @10:54:59.83 -> @10:51:59.55 Toroid.in :hold @10:54:59.86 SyntaxError in ben -- compile error ben:10: `@1' is not allowed as an instance variable name @10:55:00.04 -> @10:51:59.65 Toroid.seek :bypass @10:55:00.14 SyntaxError in ben -- compile error ben:11: `@1' is not allowed as an instance variable name @10:55:00.35 -> @10:52:01.32 Toroid.in :default @10:55:00.39 SyntaxError in ben -- compile error ben:12: `@1' is not allowed as an instance variable name @10:55:00.56 -> @10:52:01.43 Toroid.seek :clear @10:55:00.63 SyntaxError in ben -- compile error ben:13: `@1' is not allowed as an instance variable name @10:55:00.86 -> @10:52:03.07 Sampled 25.0ml @10:55:00.89 SyntaxError in ben -- compile error ben:14: `@1' is not allowed as an instance variable name @10:55:01.28 -> @10:52:03.57 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 50" @10:55:01.32 SyntaxError in ben -- compile error ben:15: `@1' is not allowed as an instance variable name @10:55:01.53 -> @10:52:05.19 SPR.command "read_sequences" @10:55:01.59 SyntaxError in ben -- compile error ben:16: `@1' is not allowed as an instance variable name @10:55:01.81 -> @10:52:05.30 SPR.command "stats_on" @10:55:01.84 SyntaxError in ben -- compile error ben:17: `@1' is not allowed as an instance variable name @10:55:02.03 -> @10:52:05.50 SPR.command "start 0 1" @10:55:02.09 SyntaxError in ben -- compile error ben:18: `@1' is not allowed as an instance variable name @10:55:02.27 -> @10:52:06.18 SPR.start /var/log/esp/spr24aug0510h.spr.bz2 @10:55:02.31 SyntaxError in ben -- compile error ben:19: `@1' is not allowed as an instance variable name @10:55:02.60 -> @10:52:06.24 Gate.power :sampler,:OFF @10:55:02.66 SyntaxError in ben -- compile error ben:20: `@1' is not allowed as an instance variable name @10:55:02.87 -> @10:52:08.10 Handoff.select :waste @10:55:02.89 SyntaxError in ben -- compile error ben:21: `@1' is not allowed as an instance variable name @10:55:03.17 -> @10:52:08.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:55:03.22 SyntaxError in ben -- compile error ben:22: `@1' is not allowed as an instance variable name @10:55:03.88 -> @10:52:08.74 upsync @10:55:03.95 SyntaxError in ben -- compile error ben:23: `@1' is not allowed as an instance variable name @10:55:04.26 -> @10:52:14.94 Syringe tare is 51% of full scale at 25C (drifted 0%) @10:55:04.34 SyntaxError in ben -- compile error ben:24: `@1' is not allowed as an instance variable name @10:55:04.54 -> @10:52:14.97 PinPort.in :locking @10:55:04.62 SyntaxError in ben -- compile error ben:25: `@1' is not allowed as an instance variable name @10:55:04.84 -> @10:52:15.08 PinPort.seek :locked @10:55:04.88 SyntaxError in ben -- compile error ben:26: `@1' is not allowed as an instance variable name @10:55:05.13 -> @10:52:15.63 Archive.in :fast @10:55:05.18 SyntaxError in ben -- compile error ben:27: `@1' is not allowed as an instance variable name @10:55:05.34 -> @10:52:15.70 Lysis2.in :fast @10:55:05.38 SyntaxError in ben -- compile error ben:28: `@1' is not allowed as an instance variable name @10:55:05.65 -> @10:52:15.81 Lysis1.seek 1350uL @10:55:05.72 SyntaxError in ben -- compile error ben:29: `@1' is not allowed as an instance variable name @10:55:05.87 -> @10:52:15.96 Archive.seek :full @10:55:05.89 SyntaxError in ben -- compile error ben:30: `@1' is not allowed as an instance variable name @10:55:06.08 -> @10:52:16.00 Lysis2.seek 950uL @10:55:06.15 SyntaxError in ben -- compile error ben:31: `@1' is not allowed as an instance variable name @10:55:06.28 -> @10:52:21.00 Plunge.seek :reagent @10:55:06.32 SyntaxError in ben -- compile error ben:32: `@1' is not allowed as an instance variable name @10:55:06.59 -> @10:52:21.94 Evacuating puck with filter bubble point of 24psi @10:55:06.67 SyntaxError in ben -- compile error ben:33: `@1' is not allowed as an instance variable name @10:55:06.81 -> @10:52:21.97 PinPort.seek :locked @10:55:06.85 SyntaxError in ben -- compile error ben:34: `@1' is not allowed as an instance variable name @10:55:07.05 -> @10:52:22.60 Handoff.select :waste @10:55:07.13 SyntaxError in ben -- compile error ben:35: `@1' is not allowed as an instance variable name @10:55:07.44 -> @10:52:22.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:55:07.56 SyntaxError in ben -- compile error ben:36: `@1' is not allowed as an instance variable name @10:55:07.77 -> @10:52:29.41 Air syringe pumping up to 1 strokes to reach 8psi @10:55:07.80 SyntaxError in ben -- compile error ben:37: `@1' is not allowed as an instance variable name @10:55:07.96 -> @10:52:29.43 Air stroke #1 of 1 @10:55:08.03 SyntaxError in ben -- compile error ben:38: `@1' is not allowed as an instance variable name @10:55:08.20 -> @10:52:29.58 Air.in :pressurize @10:55:08.23 SyntaxError in ben -- compile error ben:39: `@1' is not allowed as an instance variable name @10:55:08.45 -> @10:52:29.67 Air.seek :pressurized @10:55:08.55 SyntaxError in ben -- compile error ben:40: `@1' is not allowed as an instance variable name @10:55:08.71 -> @10:52:35.32 1.1psi after pressurize @10:55:08.75 SyntaxError in ben -- compile error ben:41: `@1' is not allowed as an instance variable name @10:55:08.85 -> @10:52:35.35 Air.in :compress @10:55:08.89 SyntaxError in ben -- compile error ben:42: `@1' is not allowed as an instance variable name @10:55:08.99 -> @10:52:35.46 Air.seek :evac @10:55:09.12 SyntaxError in ben -- compile error ben:43: `@1' is not allowed as an instance variable name @10:55:09.30 -> @10:52:38.50 1.3psi after compress @10:55:09.33 SyntaxError in ben -- compile error ben:44: `@1' is not allowed as an instance variable name @10:55:09.51 -> @10:52:49.09 0.0psi after compress (stable) @10:55:09.57 SyntaxError in ben -- compile error ben:45: `@1' is not allowed as an instance variable name @10:55:09.71 -> @10:52:49.11 Air.in :default @10:55:09.74 SyntaxError in ben -- compile error ben:46: `@1' is not allowed as an instance variable name @10:55:09.83 -> @10:52:49.27 Air.hold @10:55:09.87 SyntaxError in ben -- compile error ben:47: `@1' is not allowed as an instance variable name @10:55:10.05 -> @10:52:49.56 at -0.0psi Opened to waste @10:55:10.10 SyntaxError in ben -- compile error ben:48: `@1' is not allowed as an instance variable name @10:55:10.27 -> @10:52:49.58 Handoff.select :blocked @10:55:10.31 SyntaxError in ben -- compile error ben:49: `@1' is not allowed as an instance variable name @10:55:10.52 -> @10:52:49.76 Archive syringe pumping up to 8 strokes to reach 24psi @10:55:10.58 SyntaxError in ben -- compile error ben:50: `@1' is not allowed as an instance variable name @10:55:10.76 -> @10:52:49.77 Archive stroke #1 of 8 @10:55:10.79 SyntaxError in ben -- compile error ben:51: `@1' is not allowed as an instance variable name @10:55:20.95 Handoff.select :waste @10:55:21.21 0.4psi at evacEnd @10:55:21.23 Handoff.select :blocked @10:55:21.40 Archive.in :fast @10:55:21.52 Archive.seek :topVent @10:55:28.75 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @10:55:35.35 Stable @0.2psi after Handoff blocked @10:55:35.38 Archive syringe pumping up to 8 strokes to reach 24psi @10:55:35.42 Archive stroke #1 of 8 @10:55:35.56 Archive.in :pressurize @10:55:35.65 Archive.seek :pressurized @10:55:48.16 0.8psi after pressurize @10:55:48.20 Archive.in :compress @10:55:48.29 Archive.seek :empty @10:56:04.11 15.3psi after compress @10:56:14.74 14.3psi after compress (stable) @10:56:14.77 Archive.in :gulp @10:56:14.88 Archive.seek :topVent @10:56:21.22 15.2psi after gulp @10:56:26.86 14.4psi after gulp (stable) @10:56:26.88 Archive stroke #2 of 8 @10:56:27.03 Archive.in :pressurize @10:56:27.11 Archive.seek :pressurized @10:56:39.57 15.0psi after pressurize @10:56:39.60 Archive.in :compress @10:56:39.71 Archive.seek :empty @10:56:52.05 Reached 23.4 of 24.2psi after pumping 3382uL (1.95 strokes) @10:56:52.08 Archive.in :default @10:56:52.19 Archive.hold @10:56:52.34 Handoff.select :waste @10:56:52.53 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:56:59.66 delay 1:00 @10:57:20.42 -> timezone :timezone @10:57:59.68 Handoff.select :blocked @10:57:59.86 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @10:58:22.46 Handoff.select :waste @10:58:22.62 0.7psi at evacEnd @10:58:22.66 Air.seek :fullVent @10:58:24.08 Archive.in :fast @10:58:24.18 Archive.seek :topVent @10:58:31.10 Seawater evacuated, Pressure above/below filter released. @10:58:31.25 Lysis1.in :slow @10:58:32.00 Lysis1.seek 1050uL @10:59:01.65 delay 5 seconds @10:59:06.68 Handoff.select :blocked @10:59:06.91 Lysis1.seek 450uL @11:00:06.08 delay 3 seconds @11:00:09.18 Air.in mix with 1.57127 parts Lysis1 @11:00:10.40 Air.seek :evac @11:00:10.47 Lysis1.seek 300uL @11:00:25.48 Lysis1.seek :empty @11:00:55.21 Lysis buffer delivered @24.5psi @11:00:55.23 Polling Can every 2:00 @11:00:55.45 PuckHeater.configure :default #ambient 25.1C @11:00:55.53 PuckHeater.seek 79.6C @11:01:51.83 PuckHeater.hold 2:00,79.6C @14:03:51.72EDT05-Aug-24 Polling Can every 2:00 @14:03:52.31 PuckHeater.configure :default #ambient 25.1C @14:03:52.46 PuckHeater.hold 30 seconds,78.6C @14:04:22.66 Polling Can every 2:00 @14:04:23.23 PuckHeater.configure :default #ambient 25.1C @14:04:23.32 PuckHeater.hold 30 seconds,78.1C @14:04:53.49 Polling Can every 2:00 @14:04:54.05 PuckHeater.configure :default #ambient 25.1C @14:04:54.15 PuckHeater.hold 10:00,77.5C @14:09:56.89 -> timezone --list No source file corresponds to nil @14:09:56.94 NoMethodError in ben -- undefined method `-@' for nil:NilClass @14:14:42.49 -> 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum @18:14:42.56UTC05-Aug-24 SyntaxError in ben -- compile error ben:54: syntax error, unexpected tIDENTIFIER, expecting $end 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum ^ @18:14:42.73 -> @10:51:58.29 Abs.stop @18:14:42.75 SyntaxError in ben -- compile error ben:55: `@1' is not allowed as an instance variable name @18:14:42.90 -> @10:51:58.37 PhaseSeparator.stop @18:14:42.93 SyntaxError in ben -- compile error ben:56: `@1' is not allowed as an instance variable name @18:14:43.09 -> @10:51:58.54 Intake.close @18:14:43.14 SyntaxError in ben -- compile error ben:57: `@1' is not allowed as an instance variable name @18:14:43.25 -> @10:51:58.67 Exhaust.close @18:14:43.28 SyntaxError in ben -- compile error ben:58: `@1' is not allowed as an instance variable name @18:14:43.42 -> @10:51:59.55 Toroid.in :hold @18:14:43.44 SyntaxError in ben -- compile error ben:59: `@1' is not allowed as an instance variable name @18:14:43.57 -> @10:51:59.65 Toroid.seek :bypass @18:14:43.59 SyntaxError in ben -- compile error ben:60: `@1' is not allowed as an instance variable name @18:14:43.71 -> @10:52:01.32 Toroid.in :default @18:14:43.75 SyntaxError in ben -- compile error ben:61: `@1' is not allowed as an instance variable name @18:14:43.85 -> @10:52:01.43 Toroid.seek :clear @18:14:43.88 SyntaxError in ben -- compile error ben:62: `@1' is not allowed as an instance variable name @18:14:44.07 -> @10:52:03.07 Sampled 25.0ml @18:14:44.11 SyntaxError in ben -- compile error ben:63: `@1' is not allowed as an instance variable name @18:14:44.40 -> @10:52:03.57 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 50" @18:14:44.43 SyntaxError in ben -- compile error ben:64: `@1' is not allowed as an instance variable name @18:14:44.61 -> @10:52:05.19 SPR.command "read_sequences" @18:14:44.63 SyntaxError in ben -- compile error ben:65: `@1' is not allowed as an instance variable name @18:14:44.81 -> @10:52:05.30 SPR.command "stats_on" @18:14:44.84 SyntaxError in ben -- compile error ben:66: `@1' is not allowed as an instance variable name @18:14:45.05 -> @10:52:05.50 SPR.command "start 0 1" @18:14:45.09 SyntaxError in ben -- compile error ben:67: `@1' is not allowed as an instance variable name @18:14:45.27 -> @10:52:06.18 SPR.start /var/log/esp/spr24aug0510h.spr.bz2 @18:14:45.31 SyntaxError in ben -- compile error ben:68: `@1' is not allowed as an instance variable name @18:14:45.44 -> @10:52:06.24 Gate.power :sampler,:OFF @18:14:45.46 SyntaxError in ben -- compile error ben:69: `@1' is not allowed as an instance variable name @18:14:45.61 -> @10:52:08.10 Handoff.select :waste @18:14:45.65 SyntaxError in ben -- compile error ben:70: `@1' is not allowed as an instance variable name @18:14:45.83 -> @10:52:08.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:14:45.88 SyntaxError in ben -- compile error ben:71: `@1' is not allowed as an instance variable name @18:14:46.02 -> @10:52:08.74 upsync @18:14:46.05 SyntaxError in ben -- compile error ben:72: `@1' is not allowed as an instance variable name @18:14:46.23 -> @10:52:14.94 Syringe tare is 51% of full scale at 25C (drifted 0%) @18:14:46.25 SyntaxError in ben -- compile error ben:73: `@1' is not allowed as an instance variable name @18:14:46.37 -> @10:52:14.97 PinPort.in :locking @18:14:46.41 SyntaxError in ben -- compile error ben:74: `@1' is not allowed as an instance variable name @18:14:46.55 -> @10:52:15.08 PinPort.seek :locked @18:14:46.57 SyntaxError in ben -- compile error ben:75: `@1' is not allowed as an instance variable name @18:14:46.77 -> @10:52:15.63 Archive.in :fast @18:14:47.37 SyntaxError in ben -- compile error ben:76: `@1' is not allowed as an instance variable name @18:14:47.49 -> @10:52:15.70 Lysis2.in :fast @18:14:47.53 SyntaxError in ben -- compile error ben:77: `@1' is not allowed as an instance variable name @18:14:47.67 -> @10:52:15.81 Lysis1.seek 1350uL @18:14:47.75 SyntaxError in ben -- compile error ben:78: `@1' is not allowed as an instance variable name @18:14:47.91 -> @10:52:15.96 Archive.seek :full @18:14:47.95 SyntaxError in ben -- compile error ben:79: `@1' is not allowed as an instance variable name @18:14:48.08 -> @10:52:16.00 Lysis2.seek 950uL @18:14:48.12 SyntaxError in ben -- compile error ben:80: `@1' is not allowed as an instance variable name @18:14:48.26 -> @10:52:21.00 Plunge.seek :reagent @18:14:48.30 SyntaxError in ben -- compile error ben:81: `@1' is not allowed as an instance variable name @18:14:48.44 -> @10:52:21.94 Evacuating puck with filter bubble point of 24psi @18:14:48.47 SyntaxError in ben -- compile error ben:82: `@1' is not allowed as an instance variable name @18:14:48.59 -> @10:52:21.97 PinPort.seek :locked @18:14:48.63 SyntaxError in ben -- compile error ben:83: `@1' is not allowed as an instance variable name @18:14:48.84 -> @10:52:22.60 Handoff.select :waste @18:14:48.88 SyntaxError in ben -- compile error ben:84: `@1' is not allowed as an instance variable name @18:14:49.05 -> @10:52:22.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:14:49.09 SyntaxError in ben -- compile error ben:85: `@1' is not allowed as an instance variable name @18:14:49.26 -> @10:52:29.41 Air syringe pumping up to 1 strokes to reach 8psi @18:14:49.29 SyntaxError in ben -- compile error ben:86: `@1' is not allowed as an instance variable name @18:14:49.39 -> @10:52:29.43 Air stroke #1 of 1 @18:14:49.42 SyntaxError in ben -- compile error ben:87: `@1' is not allowed as an instance variable name @18:14:49.55 -> @10:52:29.58 Air.in :pressurize @18:14:49.62 SyntaxError in ben -- compile error ben:88: `@1' is not allowed as an instance variable name @18:14:49.75 -> @10:52:29.67 Air.seek :pressurized @18:14:49.78 SyntaxError in ben -- compile error ben:89: `@1' is not allowed as an instance variable name @18:14:49.90 -> @10:52:35.32 1.1psi after pressurize @18:14:49.94 SyntaxError in ben -- compile error ben:90: `@1' is not allowed as an instance variable name @18:14:50.03 -> @10:52:35.35 Air.in :compress @18:14:50.07 SyntaxError in ben -- compile error ben:91: `@1' is not allowed as an instance variable name @18:14:50.16 -> @10:52:35.46 Air.seek :evac @18:14:50.21 SyntaxError in ben -- compile error ben:92: `@1' is not allowed as an instance variable name @18:14:50.35 -> @10:52:38.50 1.3psi after compress @18:14:50.39 SyntaxError in ben -- compile error ben:93: `@1' is not allowed as an instance variable name @18:14:50.51 -> @10:52:49.09 0.0psi after compress (stable) @18:14:50.55 SyntaxError in ben -- compile error ben:94: `@1' is not allowed as an instance variable name @18:14:50.75 -> @10:52:49.11 Air.in :default @18:14:50.78 SyntaxError in ben -- compile error ben:95: `@1' is not allowed as an instance variable name @18:14:50.87 -> @10:52:49.27 Air.hold @18:14:50.89 SyntaxError in ben -- compile error ben:96: `@1' is not allowed as an instance variable name @18:14:51.00 -> @10:52:49.56 at -0.0psi Opened to waste @18:14:51.02 SyntaxError in ben -- compile error ben:97: `@1' is not allowed as an instance variable name @18:14:51.15 -> @10:52:49.58 Handoff.select :blocked @18:14:51.19 SyntaxError in ben -- compile error ben:98: `@1' is not allowed as an instance variable name @18:14:51.37 -> @10:52:49.76 Archive syringe pumping up to 8 strokes to reach 24psi @18:14:51.39 SyntaxError in ben -- compile error ben:99: `@1' is not allowed as an instance variable name @18:14:51.55 -> @10:52:49.77 Archive stroke #1 of 8 @18:14:51.61 SyntaxError in ben -- compile error ben:100: `@1' is not allowed as an instance variable name @18:14:53.18 Lysis complete. Syringe pressure @30.7psi @18:14:53.22 Archive syringe pumping up to 5 strokes to reach 40psi @18:14:53.25 Archive stroke #1 of 5 @18:14:53.41 Archive.in :pressurize @18:14:53.52 Archive.seek :pressurized @18:15:05.98 30.6psi after pressurize @18:15:11.59 29.7psi after pressurize (stable) @18:15:11.62 Archive.in :compress @18:15:11.73 Archive.seek :empty @18:15:20.03 Reached 41.6 of 40.0psi after pumping 1570uL (0.91 strokes) @18:15:20.06 Archive.in :default @18:15:20.16 Archive.hold @18:15:20.27 delay 2 seconds @18:15:22.30 Archive.in :fast @18:15:22.38 Archive.seek :full @18:15:29.08 Lysis1.seek 138uL @18:15:42.79 delay 1 second @18:15:43.84 Archive.seek :full @18:15:43.87 Lysis1.seek :empty @18:15:57.72 Handoff.select :blocked @18:15:57.90 Initial air pressure @43.4psi @18:15:58.00 Lysis1.in :extract @18:15:58.09 Lysis1.seek 600uL,nil @18:16:01.22 Archive syringe pumping up to 6 strokes to reach 25psi @18:16:01.24 Archive stroke #1 of 6 @18:16:01.38 Archive.in :pressurize @18:16:01.50 Archive.seek :pressurized @18:16:03.71 Archive.in :default @18:16:03.78 Archive.hold @18:16:04.15 Initial 600uL lysate @14.7psi @18:16:04.18 Handoff.select :blocked @18:16:04.36 Initial air pressure @14.2psi @18:16:04.47 Lysis1.seek 825uL,nil @18:16:04.75 Archive syringe pumping up to 6 strokes to reach 17psi @18:16:04.76 Archive stroke #1 of 6 @18:16:05.56 Archive.in :pressurize @18:16:05.76 Archive.seek :pressurized @18:16:09.35 Archive.in :default @18:16:09.43 Archive.hold @18:16:09.95 Recovered 825uL lysate @14.4psi @18:16:10.02 Air.in :halfSpeed @18:16:10.13 Air.seek :fullVent @18:16:12.18 Puck Top Vented, bottom @14.1psi @18:16:12.20 Handoff.select :waste @18:16:12.38 delay 5 seconds @18:16:17.46 Handoff open to waste, bottom @0.4psi @18:16:17.50 Air.in :default @18:16:17.63 Air.seek :evac @18:16:18.97 Lysis2.in :DAmix @18:16:19.13 Lysis1.in :DAmix @18:16:19.85 Lysis2.seek 700uL @18:16:19.95 Lysis1.seek 575uL @18:16:54.52 delay 5 seconds @18:16:59.54 Handoff.select SPR @18:16:59.70 SPR.command "start 2 14" @18:17:00.59 SPR stabilized @18:17:00.82 delay 10 seconds @18:17:10.85 SPR.command "start 2 0" @18:17:13.96 Waiting up to 11:00 for SPR to enter state 1 @18:19:11.63 SPR.command "hold_on" @18:19:13.03 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:19:13.80 Lysis2.seek 400uL @18:19:13.86 Lysis1.seek 275uL @18:19:55.39 delay 15 seconds @18:20:10.41 SPR.command "hold_off" @18:20:13.68 Waiting up to 1:00:00 for SPR to enter state 7 @18:36:25.42 -> 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum @18:36:25.50 SyntaxError in ben -- compile error ben:101: syntax error, unexpected tIDENTIFIER, expecting $end 50 filtering 2/25ml at 0.59ml/s with 25.9psi vacuum ^ @18:36:25.65 -> @10:51:58.29 Abs.stop @18:36:25.68 SyntaxError in ben -- compile error ben:102: `@1' is not allowed as an instance variable name @18:36:25.81 -> @10:51:58.37 PhaseSeparator.stop @18:36:25.84 SyntaxError in ben -- compile error ben:103: `@1' is not allowed as an instance variable name @18:36:25.97 -> @10:51:58.54 Intake.close @18:36:26.00 SyntaxError in ben -- compile error ben:104: `@1' is not allowed as an instance variable name @18:36:26.14 -> @10:51:58.67 Exhaust.close @18:36:26.17 SyntaxError in ben -- compile error ben:105: `@1' is not allowed as an instance variable name @18:36:26.30 -> @10:51:59.55 Toroid.in :hold @18:36:26.38 SyntaxError in ben -- compile error ben:106: `@1' is not allowed as an instance variable name @18:36:26.52 -> @10:51:59.65 Toroid.seek :bypass @18:36:26.55 SyntaxError in ben -- compile error ben:107: `@1' is not allowed as an instance variable name @18:36:26.68 -> @10:52:01.32 Toroid.in :default @18:36:26.74 SyntaxError in ben -- compile error ben:108: `@1' is not allowed as an instance variable name @18:36:26.86 -> @10:52:01.43 Toroid.seek :clear @18:36:26.89 SyntaxError in ben -- compile error ben:109: `@1' is not allowed as an instance variable name @18:36:27.00 -> @10:52:03.07 Sampled 25.0ml @18:36:27.04 SyntaxError in ben -- compile error ben:110: `@1' is not allowed as an instance variable name @18:36:27.32 -> @10:52:03.57 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 50" @18:36:27.36 SyntaxError in ben -- compile error ben:111: `@1' is not allowed as an instance variable name @18:36:27.53 -> @10:52:05.19 SPR.command "read_sequences" @18:36:27.56 SyntaxError in ben -- compile error ben:112: `@1' is not allowed as an instance variable name @18:36:27.68 -> @10:52:05.30 SPR.command "stats_on" @18:36:27.72 SyntaxError in ben -- compile error ben:113: `@1' is not allowed as an instance variable name @18:36:27.84 -> @10:52:05.50 SPR.command "start 0 1" @18:36:27.86 SyntaxError in ben -- compile error ben:114: `@1' is not allowed as an instance variable name @18:36:28.03 -> @10:52:06.18 SPR.start /var/log/esp/spr24aug0510h.spr.bz2 @18:36:28.06 SyntaxError in ben -- compile error ben:115: `@1' is not allowed as an instance variable name @18:36:28.24 -> @10:52:06.24 Gate.power :sampler,:OFF @18:36:28.29 SyntaxError in ben -- compile error ben:116: `@1' is not allowed as an instance variable name @18:36:28.48 -> @10:52:08.10 Handoff.select :waste @18:36:28.51 SyntaxError in ben -- compile error ben:117: `@1' is not allowed as an instance variable name @18:36:28.72 -> @10:52:08.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:36:28.76 SyntaxError in ben -- compile error ben:118: `@1' is not allowed as an instance variable name @18:36:28.86 -> @10:52:08.74 upsync @18:36:28.89 SyntaxError in ben -- compile error ben:119: `@1' is not allowed as an instance variable name @18:36:29.08 -> @10:52:14.94 Syringe tare is 51% of full scale at 25C (drifted 0%) @18:36:29.11 SyntaxError in ben -- compile error ben:120: `@1' is not allowed as an instance variable name @18:36:29.28 -> @10:52:14.97 PinPort.in :locking @18:36:29.33 SyntaxError in ben -- compile error ben:121: `@1' is not allowed as an instance variable name @18:36:29.39 SPRsummary:-863RIU,2.29e+09RIU,-542RIU,3.29RIU,none,none,none after 2813s @18:36:29.55 -> @10:52:15.08 PinPort.seek :locked @18:36:29.57 SyntaxError in ben -- compile error ben:122: `@1' is not allowed as an instance variable name @18:36:29.71 -> @10:52:15.63 Archive.in :fast @18:36:29.73 SyntaxError in ben -- compile error ben:123: `@1' is not allowed as an instance variable name @18:36:29.87 -> @10:52:15.70 Lysis2.in :fast @18:36:29.90 SyntaxError in ben -- compile error ben:124: `@1' is not allowed as an instance variable name @18:36:30.04 -> @10:52:15.81 Lysis1.seek 1350uL @18:36:30.09 SyntaxError in ben -- compile error ben:125: `@1' is not allowed as an instance variable name @18:36:30.28 -> @10:52:15.96 Archive.seek :full @18:36:30.32 SyntaxError in ben -- compile error ben:126: `@1' is not allowed as an instance variable name @18:36:30.44 -> @10:52:16.00 Lysis2.seek 950uL @18:36:30.48 SyntaxError in ben -- compile error ben:127: `@1' is not allowed as an instance variable name @18:36:30.63 -> @10:52:21.00 Plunge.seek :reagent @18:36:30.70 SyntaxError in ben -- compile error ben:128: `@1' is not allowed as an instance variable name @18:36:30.86 -> @10:52:21.94 Evacuating puck with filter bubble point of 24psi @18:36:30.91 SyntaxError in ben -- compile error ben:129: `@1' is not allowed as an instance variable name @18:36:31.02 -> @10:52:21.97 PinPort.seek :locked @18:36:31.06 SyntaxError in ben -- compile error ben:130: `@1' is not allowed as an instance variable name @18:36:31.23 -> @10:52:22.60 Handoff.select :waste @18:36:31.26 SyntaxError in ben -- compile error ben:131: `@1' is not allowed as an instance variable name @18:36:31.43 -> @10:52:22.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:36:31.46 SyntaxError in ben -- compile error ben:132: `@1' is not allowed as an instance variable name @18:36:31.62 -> @10:52:29.41 Air syringe pumping up to 1 strokes to reach 8psi @18:36:31.64 SyntaxError in ben -- compile error ben:133: `@1' is not allowed as an instance variable name @18:36:31.76 -> @10:52:29.43 Air stroke #1 of 1 @18:36:31.78 SyntaxError in ben -- compile error ben:134: `@1' is not allowed as an instance variable name @18:36:31.92 -> @10:52:29.58 Air.in :pressurize @18:36:31.95 SyntaxError in ben -- compile error ben:135: `@1' is not allowed as an instance variable name @18:36:32.10 -> @10:52:29.67 Air.seek :pressurized @18:36:32.13 SyntaxError in ben -- compile error ben:136: `@1' is not allowed as an instance variable name @18:36:32.25 -> @10:52:35.32 1.1psi after pressurize @18:36:32.29 SyntaxError in ben -- compile error ben:137: `@1' is not allowed as an instance variable name @18:36:32.39 -> @10:52:35.35 Air.in :compress @18:36:32.43 SyntaxError in ben -- compile error ben:138: `@1' is not allowed as an instance variable name @18:36:32.52 -> @10:52:35.46 Air.seek :evac @18:36:32.56 SyntaxError in ben -- compile error ben:139: `@1' is not allowed as an instance variable name @18:36:32.73 -> @10:52:38.50 1.3psi after compress @18:36:32.75 SyntaxError in ben -- compile error ben:140: `@1' is not allowed as an instance variable name @18:36:32.89 -> @10:52:49.09 0.0psi after compress (stable) @18:36:32.92 SyntaxError in ben -- compile error ben:141: `@1' is not allowed as an instance variable name @18:36:33.10 -> @10:52:49.11 Air.in :default @18:36:33.13 SyntaxError in ben -- compile error ben:142: `@1' is not allowed as an instance variable name @18:36:33.24 -> @10:52:49.27 Air.hold @18:36:33.28 SyntaxError in ben -- compile error ben:143: `@1' is not allowed as an instance variable name @18:36:33.42 -> @10:52:49.56 at -0.0psi Opened to waste @18:36:33.45 SyntaxError in ben -- compile error ben:144: `@1' is not allowed as an instance variable name @18:36:33.57 -> @10:52:49.58 Handoff.select :blocked @18:36:33.59 SyntaxError in ben -- compile error ben:145: `@1' is not allowed as an instance variable name @18:36:33.75 -> @10:52:49.76 Archive syringe pumping up to 8 strokes to reach 24psi @18:36:33.78 SyntaxError in ben -- compile error ben:146: `@1' is not allowed as an instance variable name @18:36:33.93 -> @10:52:49.77 Archive stroke #1 of 8 @18:36:33.99 SyntaxError in ben -- compile error ben:147: `@1' is not allowed as an instance variable name @18:36:58.56 -> can Can@18:36:58, 25.4C, 45% humidity, 14.4psia, 14.836V, 0.612A, 4.378Ah, 9.08W @18:37:22.52 -> @18:16:17.63 Air.seek :evac @18:37:22.55 SyntaxError in ben -- compile error ben:149: `@1' is not allowed as an instance variable name @18:37:22.68 -> @18:16:18.97 Lysis2.in :DAmix @18:37:22.72 SyntaxError in ben -- compile error ben:150: `@1' is not allowed as an instance variable name @18:37:22.83 -> @18:16:19.13 Lysis1.in :DAmix @18:37:22.87 SyntaxError in ben -- compile error ben:151: `@1' is not allowed as an instance variable name @18:37:23.00 -> @18:16:19.85 Lysis2.seek 700uL @18:37:23.02 SyntaxError in ben -- compile error ben:152: `@1' is not allowed as an instance variable name @18:37:23.24 -> @18:16:19.95 Lysis1.seek 575uL @18:37:23.27 SyntaxError in ben -- compile error ben:153: `@1' is not allowed as an instance variable name @18:37:23.39 -> @18:16:54.52 delay 5 seconds @18:37:23.44 SyntaxError in ben -- compile error ben:154: `@1' is not allowed as an instance variable name @18:37:23.58 -> @18:16:59.54 Handoff.select SPR @18:37:23.62 SyntaxError in ben -- compile error ben:155: `@1' is not allowed as an instance variable name @18:37:23.78 -> @18:16:59.70 SPR.command "start 2 14" @18:37:23.82 SyntaxError in ben -- compile error ben:156: `@1' is not allowed as an instance variable name @18:37:23.94 -> @18:17:00.59 SPR stabilized @18:37:23.97 SyntaxError in ben -- compile error ben:157: `@1' is not allowed as an instance variable name @18:37:24.14 -> @18:17:00.82 delay 10 seconds @18:37:24.18 SyntaxError in ben -- compile error ben:158: `@1' is not allowed as an instance variable name @18:37:24.31 -> @18:17:10.85 SPR.command "start 2 0" @18:37:24.34 SyntaxError in ben -- compile error ben:159: `@1' is not allowed as an instance variable name @18:37:24.50 -> @18:17:13.96 Waiting up to 11:00 for SPR to enter state 1 @18:37:24.54 SyntaxError in ben -- compile error ben:160: `@1' is not allowed as an instance variable name @18:37:24.67 -> @18:19:11.63 SPR.command "hold_on" @18:37:24.74 SyntaxError in ben -- compile error ben:161: `@1' is not allowed as an instance variable name @18:50:46.16 SPR analysis completed @18:50:54.90 Ended log spr24aug0510h.spr.bz2 @18:50:54.93 SPR.command "stats_off" @18:50:55.20 Archive.stop @18:50:55.86 Handoff.select :close @18:50:56.04 Gate.power :heat,:OFF @18:50:56.07 Gate.power :cartridge,:OFF @18:50:56.22 PinPort.in :unlocking @18:50:56.31 PinPort.seek :clear @18:50:56.82 Carousel.in :default @18:50:56.99 Carousel.home.seek 50 @18:50:57.41 Plunge.seek :locked @18:50:58.36 Archive.in :unlocking @18:50:58.52 Archive.seek :clear @18:50:58.70 Air.in :unlocking @18:50:58.87 Air.seek :clear @18:50:58.96 Lysis1.in :unlocking @18:50:59.17 Lysis1.seek :clear @18:50:59.37 Lysis2.in :unlocking @18:50:59.45 Lysis2.seek :clear @18:51:03.38 Gate.power :analytic1,:OFF @18:51:03.40 Gate.power :motor,:OFF @18:51:03.44 Gate.power :core,:OFF @18:51:03.47 Gate.power :main,:OFF @18:51:03.50 Safely stopped and ready to power off @19:04:11.94 -> MainThread.exit # @19:04:12.10 Session Ended @19:05:15.13UTC05-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @19:05:16.94 >BREAK< ESP3 Gateway v1.43 for core bus @19:05:16.96 >BREAK< ESP3 Gateway v1.43 for cartridge bus @19:05:21.17 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @19:05:21.93 Sea pressure (Abs) tare is 6% of full scale @19:05:36.95 Polling Can every 2:00 @19:05:37.01 Waiting for espclients... @19:05:38.02 Session Begun Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @19:10:14.57 Session Begun @19:10:37.59 -> showlog @19:11:21.90 -> MainThread.exit # @19:11:22.05 ThreadError in ben#2 -- killed thread @19:11:22.16 Session Ended @19:11:22.21 Session Ended @19:12:02.91UTC05-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @19:12:04.87 >BREAK< ESP3 Gateway v1.43 for core bus @19:12:04.90 >BREAK< ESP3 Gateway v1.43 for cartridge bus @19:12:09.05 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @19:12:09.87 Sea pressure (Abs) tare is 6% of full scale @19:12:25.23 Polling Can every 2:00 @19:12:25.29 Waiting for espclients... @19:12:26.05 Session Begun @19:12:51.71 -> showlog @19:15:05.62 -> exit @19:15:05.65 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @19:24:49.79 Session Begun @19:25:31.11 -> deep; fire lysis # @19:25:31.36 Gate.power :sampler,:OFF,limit to 3500mA @19:25:31.39 Gate.power :skimmer,:OFF,limit to 3500mA @19:25:31.43 Gate.power :main,:ON,limit to 5000mA @19:25:31.47 Gate.power :analytic1,:ON,limit to 4000mA @19:25:31.67 Powering core bus @19:25:33.92 SPR Module 1, FW Rev. B, FPGA Rev. C @19:25:34.46 Plunge.configure :default @19:25:34.57 SamplePump.configure :default @19:25:34.70 Toroid.configure :default @19:25:34.81 Carousel.configure :default @19:25:34.92 Abs.configure :default @19:25:35.02 PhaseSeparator.configure :default @19:25:35.13 Archive.configure :default @19:25:35.23 Air.configure :default @19:25:35.31 Lysis1.configure :default @19:25:35.38 Lysis2.configure :default @19:25:35.49 PinPort.configure :default @19:25:35.59 All elf controllers running firmware version 4.92 @19:25:35.64 Gate.power :motor,:ON,limit to 5000mA @19:25:36.02 Plunge.home.jog 0 @19:25:36.72 Lysis2.home.jog 0 @19:25:36.79 Archive.home.jog 0 @19:25:36.83 Lysis1.home.jog 0 @19:25:36.86 Air.home.jog 0 @19:25:36.91 Carousel.home.jog 0 @19:25:36.95 PinPort.home.jog 0 @19:25:36.99 Toroid.home.jog 0 @19:25:38.33 Plunge.in :homing @19:25:38.58 PinPort.in :homing @19:25:38.65 Plunge.jog 1611 @19:25:38.74 Lysis1.in :homing @19:25:38.77 Archive.in :homing @19:25:38.83 Air.in :homing @19:25:38.90 Toroid.in :homing @19:25:38.97 Lysis2.in :homing @19:25:39.15 PinPort.jog -108 @19:25:39.31 Lysis1.jog 1398 @19:25:39.35 Air.jog 1365 @19:25:39.42 Archive.jog 1398 @19:25:39.57 Lysis2.jog 1405 @19:25:39.74 Toroid.jog 630 @19:25:39.94 PinPort.setRawPosition! 0 @19:25:40.02 PinPort.seek :home @19:25:40.14 PinPort.in :default @19:25:40.27 Plunge.setRawPosition! 50 @19:25:40.35 Plunge.seek :home @19:25:40.69 Plunge.in :default @19:25:40.81 Lysis1.setRawPosition! 50 @19:25:40.89 Lysis1.seek :home @19:25:41.04 Air.setRawPosition! 50 @19:25:41.07 Archive.setRawPosition! 50 @19:25:41.11 Lysis2.setRawPosition! 50 @19:25:41.32 Air.seek :home @19:25:41.36 Archive.seek :home @19:25:41.41 Lysis2.seek :home @19:25:41.69 Lysis1.in :default @19:25:41.86 Archive.in :default @19:25:41.88 Air.in :default @19:25:42.08 Lysis2.in :default @19:25:42.10 Toroid.setRawPosition! 0 @19:25:42.25 Toroid.seek :home @19:25:42.38 Toroid.in :default @19:25:42.52 Toroid.seek :clear @19:25:44.29 Carousel.in :default @19:25:44.44 Carousel.home.seek 50 @19:25:44.79 Selecting Cartridge 48 @19:25:45.11 Carousel.seek 48 @19:25:45.88 Lysis1.in :fast @19:25:46.01 Lysis1.seek :locked @19:25:48.70 Plunge.seek :locked @19:25:48.89 Air.in :fast @19:25:48.96 Air.seek :locked @19:25:49.97 Carousel.reconfigure :locked @19:25:50.15 Powering cartridge bus @19:25:52.82 Gate.setIR 1750,5000,0,6 @19:25:52.95 Gate.setIR 0,5000,0,6 @19:25:53.04 PuckHeater.configure :default @19:25:53.13 Gate.power :heat,:ON,limit to 5000mA @19:25:53.90 Mounted lysis Cartridge board#Q05062 @19:25:53.96 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:25:54.51 PhytoSampler with {source: :sampler} @19:25:54.60 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 48" @19:25:55.01 Preparing to filter cartridge 48 @19:25:55.31 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @19:25:59.78 upsync @19:26:25.95 Intake Head Pressure tare is 4% of full scale at 25C (drifted 3%) @19:26:25.98 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @19:26:26.04 PhaseSeparator.in :filling @19:26:26.11 PhaseSeparator.hold @19:26:26.21 Gate.power :sampler,:ON @19:26:33.25 Head pressure reached 1.78psi in 8 seconds @19:26:33.30 Air.in :default @19:26:33.49 Air.seek :sealed @19:26:33.53 Priming sample loop w/150ml, bypass w/50ml @19:26:33.61 SamplePump.setPosition! 0ml @19:26:33.71 Bypass.open @19:26:35.20 Intake.open @19:26:36.02 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:26:38.66 Vacuum tare is 51% of full scale at 25C (drifted 0%) @19:26:38.85 Abs.in :default @19:26:38.94 Abs.hold @19:26:39.05 PhaseSeparator.in :full @19:26:39.13 PhaseSeparator.hold @19:26:39.39 Waiting for sampler to finish priming... @19:26:39.41 delayUntil "19:27:56.23" @19:27:56.30 SamplePump.in :start @19:27:56.45 SamplePump.seek 50ml,3:50 @19:27:56.99 SamplePump.configure :prime @19:28:57.86 Abs.stop @19:28:57.94 PhaseSeparator.stop @19:28:58.03 SamplePump.setPosition! 0ml @19:28:58.11 Exhaust.open @19:28:59.46 Bypass.close @19:29:00.99 Abs.in :default @19:29:01.07 Abs.hold @19:29:01.19 PhaseSeparator.hold @19:29:01.55 SamplePump.in :start @19:29:01.64 SamplePump.seek 150ml,10:30 @19:29:02.24 SamplePump.configure :prime @19:32:05.15 Abs.stop @19:32:05.29 PhaseSeparator.stop @19:32:05.39 Bypass.open @19:32:06.57 Intake.close @19:32:07.41 Toroid.in :hold @19:32:07.49 Toroid.seek :crack @19:32:09.06 Toroid.seek :cartridge @19:32:10.21 Toroid.in :default @19:32:10.30 Toroid.seek :clear @19:32:12.46 Pressurizing cartridge to 26psi above 13.9psia @19:32:12.48 SamplePump.setPosition! 0ml @19:32:12.57 Intake.open @19:32:13.28 Bypass.close @19:32:13.94 Exhaust.close @19:32:14.76 Abs.in :default @19:32:14.85 Abs.hold @19:32:14.95 PhaseSeparator.hold @19:32:15.23 SamplePump.in :charge @19:32:15.32 SamplePump.seek 2.3ml,42 seconds @19:32:28.95 Abs.stop @19:32:29.04 PhaseSeparator.stop @19:32:29.12 Intake.close @19:32:29.89 Pressurized cartridge with 0.833ml to 27.1psi @19:32:29.90 Isolated @36.7psia (22.8psi) @19:32:29.94 Verifying cartridge holds at least 0.65..0.85 of 36.7psia @19:32:30.01 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:32:34.62 Cartridge pressure fell 1% to 36.4psia @19:32:34.79 Sampling 25.0ml @19:32:34.80 SamplePump.setPosition! 0ml @19:32:34.90 Exhaust.open @19:32:35.55 Intake.open @19:32:36.42 Abs.in :default @19:32:36.51 Abs.hold @19:32:36.60 PhaseSeparator.hold @19:32:37.02 SamplePump.in :start @19:32:37.12 SamplePump.seek 25ml,2:35 @19:32:37.71 SamplePump.configure :sample @19:32:40.11 Cartridge 48 filtering 2/25ml at 0.76ml/s with 24.6psi vacuum @19:33:18.37 Abs.stop @19:33:18.45 PhaseSeparator.stop @19:33:18.64 Intake.close @19:33:18.72 Exhaust.close @19:33:19.02 Toroid.in :hold @19:33:19.10 Toroid.seek :bypass @19:33:21.29 Toroid.in :default @19:33:21.37 Toroid.seek :clear @19:33:23.12 Sampled 25.0ml @19:33:23.60 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 48" @19:33:25.33 SPR.command "read_sequences" @19:33:25.79 SPR.command "stats_on" @19:33:26.00 SPR.command "start 0 1" @19:33:26.28 Gate.power :sampler,:OFF @19:33:26.71 SPR.start /var/log/esp/spr24aug0519h.spr.bz2 @19:33:28.71 Handoff.select :waste @19:33:28.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:33:29.34 upsync @19:33:35.55 Syringe tare is 51% of full scale at 25C (drifted 0%) @19:33:35.61 PinPort.in :locking @19:33:35.72 PinPort.seek :locked @19:33:36.27 Archive.in :fast @19:33:36.36 Lysis2.in :fast @19:33:36.51 Lysis1.seek 1350uL @19:33:36.56 Archive.seek :full @19:33:36.61 Lysis2.seek 950uL @19:33:41.57 Plunge.seek :reagent @19:33:42.53 Evacuating puck with filter bubble point of 24psi @19:33:42.56 PinPort.seek :locked @19:33:42.74 Handoff.select :waste @19:33:42.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:33:49.40 Air syringe pumping up to 1 strokes to reach 8psi @19:33:49.41 Air stroke #1 of 1 @19:33:49.56 Air.in :pressurize @19:33:49.65 Air.seek :pressurized @19:33:55.30 0.8psi after pressurize @19:33:55.34 Air.in :compress @19:33:55.42 Air.seek :evac @19:33:58.52 1.7psi after compress @19:34:12.12 1.6psi after compress (stable) @19:34:12.14 Air.in :default @19:34:12.25 Air.hold @19:34:12.49 at 1.1psi Opened to waste @19:34:12.51 Handoff.select :blocked @19:34:12.74 Archive syringe pumping up to 8 strokes to reach 24psi @19:34:12.75 Archive stroke #1 of 8 @19:34:12.88 Archive.in :pressurize @19:34:12.98 Archive.seek :pressurized @19:34:25.95 3.1psi after pressurize @19:34:31.61 2.6psi after pressurize (stable) @19:34:31.65 Archive.in :compress @19:34:31.76 Archive.seek :empty @19:34:47.53 15.3psi after compress @19:34:58.12 15.0psi after compress (stable) @19:34:58.15 Archive.in :gulp @19:34:58.25 Archive.seek :topVent @19:35:04.63 15.9psi after gulp @19:35:10.31 15.3psi after gulp (stable) @19:35:10.34 Archive stroke #2 of 8 @19:35:10.49 Archive.in :pressurize @19:35:10.59 Archive.seek :pressurized @19:35:23.03 15.9psi after pressurize @19:35:28.63 15.2psi after pressurize (stable) @19:35:28.70 Archive.in :compress @19:35:28.82 Archive.seek :empty @19:35:40.55 Reached 24.3 of 24.0psi after pumping 3373uL (1.95 strokes) @19:35:40.59 Archive.in :default @19:35:40.74 Archive.hold @19:35:40.84 Handoff.select :waste @19:35:41.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:35:54.60 delay 1:00 @19:36:54.62 Handoff.select :blocked @19:36:54.83 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:37:17.43 Handoff.select :waste @19:37:17.59 0.7psi at evacEnd @19:37:17.61 Handoff.select :blocked @19:37:17.78 Archive.in :fast @19:37:17.86 Archive.seek :topVent @19:37:24.76 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @19:37:31.36 Stable @0.3psi after Handoff blocked @19:37:31.39 Archive syringe pumping up to 8 strokes to reach 24psi @19:37:31.42 Archive stroke #1 of 8 @19:37:31.56 Archive.in :pressurize @19:37:31.65 Archive.seek :pressurized @19:37:44.11 1.1psi after pressurize @19:37:44.14 Archive.in :compress @19:37:44.25 Archive.seek :empty @19:37:59.99 15.3psi after compress @19:38:10.58 14.4psi after compress (stable) @19:38:10.63 Archive.in :gulp @19:38:10.76 Archive.seek :topVent @19:38:17.11 15.4psi after gulp @19:38:22.74 14.8psi after gulp (stable) @19:38:22.75 Archive stroke #2 of 8 @19:38:22.90 Archive.in :pressurize @19:38:22.99 Archive.seek :pressurized @19:38:35.44 15.3psi after pressurize @19:38:35.47 Archive.in :compress @19:38:35.61 Archive.seek :empty @19:38:47.39 Reached 24.4 of 24.3psi after pumping 3375uL (1.95 strokes) @19:38:47.42 Archive.in :default @19:38:47.53 Archive.hold @19:38:47.63 Handoff.select :waste @19:38:47.79 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:38:55.42 delay 1:00 @19:39:55.47 Handoff.select :blocked @19:39:55.64 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:40:18.24 Handoff.select :waste @19:40:18.40 0.7psi at evacEnd @19:40:18.42 Air.seek :fullVent @19:40:19.78 Archive.in :fast @19:40:19.88 Archive.seek :topVent @19:40:26.76 Seawater evacuated, Pressure above/below filter released. @19:40:26.89 Lysis1.in :slow @19:40:26.98 Lysis1.seek 1050uL @19:40:56.62 delay 5 seconds @19:41:01.64 Handoff.select :blocked @19:41:01.88 Lysis1.seek 450uL @19:42:01.08 delay 3 seconds @19:42:04.18 Air.in mix with 1.57127 parts Lysis1 @19:42:04.92 Air.seek :evac @19:42:05.05 Lysis1.seek 300uL @19:42:19.99 Lysis1.seek :empty @19:42:49.72 Lysis buffer delivered @20.8psi @19:42:49.74 Polling Can every 2:00 @19:42:50.37 PuckHeater.configure :default #ambient 25.7C @19:42:50.45 PuckHeater.seek 79.6C @19:43:32.35 Session Begun @19:43:36.56 -> showlog @19:43:45.85 PuckHeater.hold 2:00,79.6C @19:44:07.71 -> can Can@19:44:07, 25.7C, 45% humidity, 14.5psia, 14.778V, 1.272A, 4.947Ah, 18.80W @19:45:45.90 Polling Can every 2:00 @19:45:46.47 PuckHeater.configure :default #ambient 25.8C @19:45:46.58 PuckHeater.hold 30 seconds,78.6C @19:46:16.85 Polling Can every 2:00 @19:46:17.40 PuckHeater.configure :default #ambient 25.8C @19:46:17.51 PuckHeater.hold 30 seconds,78C @19:46:47.75 Polling Can every 2:00 @19:46:48.31 PuckHeater.configure :default #ambient 25.8C @19:46:48.51 PuckHeater.hold 10:00,77.5C @19:56:48.21 Lysis complete. Syringe pressure @29.1psi @19:56:48.24 Archive syringe pumping up to 5 strokes to reach 40psi @19:56:48.29 Archive stroke #1 of 5 @19:56:48.45 Archive.in :pressurize @19:56:48.55 Archive.seek :pressurized @19:57:01.02 29.0psi after pressurize @19:57:06.62 28.4psi after pressurize (stable) @19:57:06.66 Archive.in :compress @19:57:06.82 Archive.seek :empty @19:57:16.03 Reached 41.5 of 40.0psi after pumping 1590uL (0.92 strokes) @19:57:16.05 Archive.in :default @19:57:16.18 Archive.hold @19:57:16.30 delay 2 seconds @19:57:18.33 Archive.in :fast @19:57:18.43 Archive.seek :full @19:57:25.22 Lysis1.seek 138uL @19:57:27.82 Session Ended @19:57:38.95 delay 1 second @19:57:40.01 Archive.seek :full @19:57:40.07 Lysis1.seek :empty @19:57:53.85 Handoff.select :blocked @19:57:54.01 Initial air pressure @50.7psi @19:57:54.09 Lysis1.in :extract @19:57:54.24 Lysis1.seek 600uL,nil @19:57:56.81 Archive syringe pumping up to 6 strokes to reach 25psi @19:57:56.83 Archive stroke #1 of 6 @19:57:56.98 Archive.in :pressurize @19:57:57.07 Archive.seek :pressurized @19:58:09.54 15.5psi after pressurize @19:58:15.16 15.1psi after pressurize (stable) @19:58:15.19 Archive.in :compress @19:58:15.29 Archive.seek :empty @19:58:25.04 Archive.in :default @19:58:25.11 Archive.hold @19:58:25.32 Initial 600uL lysate @16.9psi @19:58:25.35 Handoff.select :blocked @19:58:25.53 Initial air pressure @17.9psi @19:58:25.65 Lysis1.seek 825uL,nil @19:58:26.45 Archive syringe pumping up to 6 strokes to reach 17psi @19:58:26.47 Archive stroke #1 of 6 @19:58:26.62 Archive.in :compress @19:58:26.75 Archive.seek :empty @19:58:30.29 Archive.in :default @19:58:30.37 Archive.hold @19:58:30.85 Recovered 825uL lysate @16.9psi @19:58:30.87 Air.in :halfSpeed @19:58:30.98 Air.seek :fullVent @19:58:33.06 Puck Top Vented, bottom @23.6psi @19:58:33.08 Handoff.select :waste @19:58:33.25 delay 5 seconds @19:58:38.33 Handoff open to waste, bottom @0.6psi @19:58:38.35 Air.in :default @19:58:38.47 Air.seek :evac @19:58:39.81 Lysis2.in :DAmix @19:58:39.96 Lysis1.in :DAmix @19:58:41.20 Lysis2.seek 700uL @19:58:41.25 Lysis1.seek 575uL @19:59:15.90 delay 5 seconds @19:59:20.92 Handoff.select SPR @19:59:21.09 SPR.command "start 2 14" @19:59:21.69 SPR stabilized @19:59:21.89 delay 10 seconds @19:59:31.91 SPR.command "start 2 0" @19:59:35.06 Waiting up to 11:00 for SPR to enter state 1 @20:01:32.72 SPR.command "hold_on" @20:01:34.12 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:01:34.88 Lysis2.seek 400uL @20:01:34.95 Lysis1.seek 275uL @20:02:16.47 delay 15 seconds @20:02:31.49 SPR.command "hold_off" @20:02:34.76 Waiting up to 1:00:00 for SPR to enter state 7 @20:18:50.34 SPRsummary:46.7RIU,306RIU,133RIU,38.6RIU,none,332ng/L,high after 2877s @20:33:07.24 SPR analysis completed @20:33:16.30 Ended log spr24aug0519h.spr.bz2 @20:33:16.32 SPR.command "stats_off" @20:33:17.31 Handoff.select :close @20:33:17.49 Gate.power :heat,:OFF @20:33:17.51 Gate.power :cartridge,:OFF @20:33:17.64 PinPort.in :unlocking @20:33:17.72 PinPort.seek :clear @20:33:18.23 Carousel.in :default @20:33:18.38 Carousel.home.seek 48 @20:33:18.77 Plunge.seek :locked @20:33:19.69 Archive.in :unlocking @20:33:19.85 Archive.seek :clear @20:33:19.99 Air.in :unlocking @20:33:20.67 Air.seek :clear @20:33:20.82 Lysis1.in :unlocking @20:33:20.99 Lysis1.seek :clear @20:33:21.13 Lysis2.in :unlocking @20:33:21.24 Lysis2.seek :clear @20:33:25.12 Gate.power :analytic1,:OFF @20:33:25.14 Gate.power :motor,:OFF @20:33:25.17 Gate.power :core,:OFF @20:33:25.22 Gate.power :main,:OFF @20:33:25.25 Safely stopped and ready to power off @20:34:03.91 -> cd var/log/esp @20:34:03.95 NoMethodError in bill -- undefined method `/' for :var:Symbol @20:34:08.06 -> exit @20:34:08.09 Session Ended @20:56:01.38 Session Begun @20:56:16.69 -> Cartridge.release55 @20:56:16.74 NoMethodError in bill -- undefined method `release55' for Cartridge @20:56:34.29 -> Cartridge.release 55 1 @20:56:40.36 -> runStandard # @20:56:40.60 Gate.power :sampler,:OFF,limit to 3500mA @20:56:40.63 Gate.power :skimmer,:OFF,limit to 3500mA @20:56:40.74 Gate.power :main,:ON,limit to 5000mA @20:56:40.79 Gate.power :analytic1,:ON,limit to 4000mA @20:56:40.90 Powering core bus @20:56:43.25 SPR Module 1, FW Rev. B, FPGA Rev. C @20:56:43.69 Plunge.configure :default @20:56:43.80 SamplePump.configure :default @20:56:43.90 Toroid.configure :default @20:56:44.01 Carousel.configure :default @20:56:44.10 Abs.configure :default @20:56:44.21 PhaseSeparator.configure :full @20:56:44.32 Archive.configure :default @20:56:44.43 Air.configure :default @20:56:44.50 Lysis1.configure :default @20:56:44.58 Lysis2.configure :default @20:56:44.72 PinPort.configure :default @20:56:44.81 All elf controllers running firmware version 4.92 @20:56:44.83 Gate.power :motor,:ON,limit to 5000mA @20:56:45.20 Plunge.home.jog 0 @20:56:45.84 Lysis2.home.jog 0 @20:56:45.92 Lysis1.home.jog 0 @20:56:45.97 Air.home.jog 0 @20:56:46.02 Archive.home.jog 0 @20:56:46.06 Toroid.home.jog 0 @20:56:46.11 Carousel.home.jog 0 @20:56:46.15 PinPort.home.jog 0 @20:56:47.24 Plunge.in :homing @20:56:47.54 Plunge.jog 1607 @20:56:47.62 Lysis2.in :homing @20:56:47.67 Air.in :homing @20:56:47.71 Toroid.in :homing @20:56:47.76 PinPort.in :homing @20:56:47.79 Archive.in :homing @20:56:47.83 Lysis1.in :homing @20:56:48.21 Lysis2.jog 1404 @20:56:48.27 Air.jog 1372 @20:56:48.31 PinPort.jog -108 @20:56:48.38 Toroid.jog 616 @20:56:48.42 Lysis1.jog 1400 @20:56:48.48 Archive.jog 1401 @20:56:48.97 PinPort.setRawPosition! 0 @20:56:49.06 PinPort.seek :home @20:56:49.21 Plunge.setRawPosition! 50 @20:56:49.23 PinPort.in :default @20:56:49.39 Plunge.seek :home @20:56:49.71 Plunge.in :default @20:56:49.82 Air.setRawPosition! 50 @20:56:49.87 Lysis2.setRawPosition! 50 @20:56:50.01 Air.seek :home @20:56:50.07 Lysis2.seek :home @20:56:50.80 Lysis2.in :default @20:56:50.84 Lysis1.setRawPosition! 50 @20:56:50.87 Archive.setRawPosition! 50 @20:56:50.93 Air.in :default @20:56:51.12 Lysis1.seek :home @20:56:51.22 Archive.seek :home @20:56:51.53 Toroid.setRawPosition! 0 @20:56:51.65 Toroid.seek :home @20:56:51.83 Archive.in :default @20:56:51.86 Toroid.in :default @20:56:51.90 Lysis1.in :default @20:56:52.34 Toroid.seek :clear @20:56:54.08 Carousel.in :default @20:56:54.25 Carousel.home.seek 48 @20:56:54.54 Selecting Cartridge 55 @20:56:54.89 Carousel.seek 55 @20:56:56.72 Lysis1.in :fast @20:56:56.81 Lysis1.seek :locked @20:56:59.46 Plunge.seek :locked @20:56:59.66 Air.in :fast @20:56:59.75 Air.seek :locked @20:57:00.75 Carousel.reconfigure :locked @20:57:00.93 Powering cartridge bus @20:57:03.60 Gate.setIR 1750,5000,0,6 @20:57:03.73 Gate.setIR 0,5000,0,6 @20:57:03.83 PuckHeater.configure :default #ambient 25.8C @20:57:03.92 Gate.power :heat,:ON,limit to 5000mA @20:57:04.54 Mounted lysis Cartridge @20:57:04.60 WARNING: Using default puck temperature sensor calibration @20:57:04.62 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:57:05.04 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 55" @20:57:06.65 SPR.command "read_sequences" @20:57:06.79 SPR.command "stats_on" @20:57:06.99 SPR.command "start 0 1" @20:57:07.70 SPR.start /var/log/esp/spr24aug0520h.spr.bz2 @20:57:10.14 Waiting for baseline SPR stabilization @20:57:10.16 Handoff.select :waste @20:57:10.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:57:10.40 upsync @20:57:17.01 Syringe tare is 52% of full scale at 26C (drifted 0%) @20:57:17.05 PinPort.in :locking @20:57:17.15 PinPort.seek :locked @20:57:17.70 Archive.in :fast @20:57:17.84 Lysis2.in :fast @20:57:17.91 Lysis1.seek 1100uL @20:57:17.97 Archive.seek :full @20:57:18.10 Lysis2.seek 1100uL @20:57:22.45 delayUntil "21:07:10.10" @21:07:10.12 Lysis2.in :DAmix @21:07:10.26 Lysis1.in :DAmix @21:07:10.90 Lysis2.seek 850uL @21:07:10.97 Lysis1.seek 850uL @21:07:45.65 delay 5 seconds @21:07:50.68 Handoff.select SPR @21:07:50.84 SPR.command "start 2 14" @21:07:51.86 SPR stabilized @21:07:52.05 delay 10 seconds @21:08:02.07 SPR.command "start 2 0" @21:08:05.73 Waiting up to 11:00 for SPR to enter state 1 @21:10:03.83 SPR.command "hold_on" @21:10:05.23 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:10:05.90 Lysis2.seek 550uL @21:10:06.01 Lysis1.seek 550uL @21:10:47.51 delay 15 seconds @21:11:02.53 SPR.command "hold_off" @21:11:06.39 Waiting up to 1:00:00 for SPR to enter state 7 @21:27:22.39 SPRsummary:-7.98RIU,175RIU,185RIU,-10.3RIU after 1916s @21:41:39.44 delay 10 seconds @21:41:49.46 SPR.command "start 2 0" @21:41:52.15 Waiting up to 11:00 for SPR to enter state 1 @21:43:49.81 SPR.command "hold_on" @21:43:51.21 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:43:51.92 Lysis2.seek 400uL @21:43:52.02 Lysis1.seek 400uL @21:44:12.87 delay 15 seconds @21:44:27.89 SPR.command "hold_off" @21:44:31.56 Waiting up to 1:00:00 for SPR to enter state 7 @22:15:04.60 delay 10 seconds @22:15:14.62 SPR.command "start 2 0" @22:15:17.31 Waiting up to 11:00 for SPR to enter state 1 @22:17:14.97 SPR.command "hold_on" @22:17:16.37 SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:17:17.09 Lysis2.seek 250uL @22:17:17.21 Lysis1.seek 250uL @22:17:38.04 delay 15 seconds @22:17:53.06 SPR.command "hold_off" @22:17:56.21 Waiting up to 1:00:00 for SPR to enter state 7 @22:31:18.61 Session Ended @22:48:28.71 SPR analysis completed @22:48:44.81 Ended log spr24aug0520h.spr.bz2 @22:48:44.82 SPR.command "stats_off" @23:00:08.98 Session Begun @23:00:11.41 -> sowlog :sowlog @23:00:15.71 -> showlog @23:00:24.77 -> showlog -50 @23:00:50.22 -> exit @23:00:50.24 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @00:05:28.77UTC06-Aug-24 Session Begun @00:05:41.16 -> ESP.ready! @00:05:41.99 Toroid.seek :clear @00:05:42.19 Carousel.in :default @00:05:42.32 Carousel.home.seek 55 @00:05:42.78 Carousel.reconfigure :locked @00:05:43.03 Gate.setIR 1750,5000,0,6 @00:05:43.17 Gate.setIR 0,5000,0,6 @00:05:43.26 PuckHeater.configure :default #ambient 25.8C @00:05:43.36 Gate.power :heat,:ON,limit to 5000mA @00:05:43.98 Mounted lysis Cartridge @00:05:44.49 WARNING: Using default puck temperature sensor calibration @00:05:44.54 Using default cartridge heat loss curve for HeatingAccelInCarousel Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects Analytical Module SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.00psi Archive = Archive Syringe (ArS) full Air = Air Syringe (AirS) full Lysis1 = Lysis Syringe #1 (LyS1) at 250uL Lysis2 = Lysis Syringe #2 (LyS2) at 250uL PinPort = Handoff Pin Port (PP) cartridge PuckHeater = Puck Heater at 72.6C @00:06:55.31 -> require 'vacuumCart' true @00:07:15.39 -> Power.sampler :ON @00:07:15.40 Gate.power :sampler,:ON true @00:07:49.23 -> 3.times{refillSampleLines} @00:07:49.26 Refilling sample & bypass lines with fluid. @00:07:50.12 Toroid.seek :clear @00:07:50.33 Carousel.in :default @00:07:50.47 Carousel.home.seek 55 @00:07:50.92 Carousel.reconfigure :locked @00:07:51.17 Gate.setIR 1750,5000,0,6 @00:07:51.30 Gate.setIR 0,5000,0,6 @00:07:51.40 PuckHeater.configure :default #ambient 25.8C @00:07:51.49 Gate.power :heat,:ON,limit to 5000mA @00:07:52.11 Mounted lysis Cartridge @00:07:52.16 WARNING: Using default puck temperature sensor calibration @00:07:52.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:07:52.27 Waiting for regs to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @00:08:08.03 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @00:08:08.05 Bypass.open @00:08:08.76 Intake.open @00:08:09.44 SamplePump.setPosition! 0ml @00:08:09.58 SamplePump.seek 30ml @00:08:46.79 Bypass.close @00:08:47.48 Exhaust.open @00:08:48.26 SamplePump.seek 55ml @00:09:19.37 delay 1 second @00:09:20.40 Intake.close @00:09:20.45 Exhaust.close @00:09:20.61 Handoff.select :close @00:09:21.25 Sampling Loop filled with water @00:09:21.28 Refilling sample & bypass lines with fluid. @00:09:22.00 Toroid.seek :clear @00:09:22.22 Carousel.in :default @00:09:22.38 Carousel.home.seek 55 @00:09:22.84 Carousel.reconfigure :locked @00:09:23.50 Gate.setIR 1750,5000,0,6 @00:09:23.74 Gate.setIR 0,5000,0,6 @00:09:23.85 PuckHeater.configure :default #ambient 25.8C @00:09:23.95 Gate.power :heat,:ON,limit to 5000mA @00:09:24.57 Mounted lysis Cartridge @00:09:24.63 WARNING: Using default puck temperature sensor calibration @00:09:24.67 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:09:24.76 Waiting for regs to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @00:09:45.72 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @00:09:45.74 Bypass.open @00:09:46.48 Intake.open @00:09:47.18 SamplePump.setPosition! 0ml @00:09:47.32 SamplePump.seek 30ml @00:10:24.52 Bypass.close @00:10:25.23 Exhaust.open @00:10:25.99 SamplePump.seek 55ml @00:10:57.10 delay 1 second @00:10:58.14 Intake.close @00:10:58.22 Exhaust.close @00:10:59.01 Sampling Loop filled with water @00:10:59.03 Refilling sample & bypass lines with fluid. @00:10:59.97 Toroid.seek :clear @00:11:00.19 Carousel.in :default @00:11:00.34 Carousel.home.seek 55 @00:11:00.78 Carousel.reconfigure :locked @00:11:01.03 Gate.setIR 1750,5000,0,6 @00:11:01.16 Gate.setIR 0,5000,0,6 @00:11:01.25 PuckHeater.configure :default #ambient 25.8C @00:11:01.35 Gate.power :heat,:ON,limit to 5000mA @00:11:01.97 Mounted lysis Cartridge @00:11:02.03 WARNING: Using default puck temperature sensor calibration @00:11:02.05 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:11:02.14 Waiting for regs to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @00:11:08.84 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @00:11:08.85 Bypass.open @00:11:09.56 Intake.open @00:11:10.29 SamplePump.setPosition! 0ml @00:11:10.42 SamplePump.seek 30ml @00:11:47.63 Bypass.close @00:11:48.28 Exhaust.open @00:11:49.04 SamplePump.seek 55ml @00:12:20.15 delay 1 second @00:12:21.18 Intake.close @00:12:21.23 Exhaust.close @00:12:22.02 Sampling Loop filled with water 3 @00:12:38.44 -> BACsampler.source = sampler :sampler @00:12:50.60 -> BACsampler.prime n,n @00:12:50.74 Priming sample loop w/nml, bypass w/nml @00:12:50.76 SamplePump.setPosition! 0ml @00:12:50.86 Bypass.open @00:12:52.31 Intake.open @00:12:53.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:12:55.82 Vacuum tare is 52% of full scale at 26C (drifted 1%) @00:12:56.00 Abs.in :default @00:12:56.08 Abs.hold @00:12:56.20 PhaseSeparator.hold @00:12:56.45 SamplePump.stop @00:12:56.53 Intake.close @00:12:56.70 Bypass.close @00:12:57.49 Abs.stop @00:12:57.57 PhaseSeparator.stop @00:12:57.67 NoMethodError in regs -- undefined method `-' for :n:Symbol @00:13:28.78 -> Power.sampler :OFF @00:13:28.80 Gate.power :sampler,:OFF false @00:13:55.63 -> deep; fire lysis # @00:13:55.84 Selecting Cartridge 46 @00:13:56.08 Gate.power :heat,:OFF @00:13:56.10 Gate.power :cartridge,:OFF @00:13:56.23 PinPort.in :unlocking @00:13:56.32 PinPort.seek :clear @00:13:56.83 Carousel.in :default @00:13:57.00 Carousel.home.seek 55 @00:13:57.37 Archive.in :unlocking @00:13:57.52 Archive.seek :clear @00:13:57.65 Air.in :unlocking @00:13:57.82 Air.seek :clear @00:13:57.94 Lysis2.in :unlocking @00:13:58.06 Lysis2.seek :clear @00:14:02.14 Lysis1.in :unlocking @00:14:02.30 Lysis1.seek :clear @00:14:02.42 Plunge.in :unlocking @00:14:02.51 Plunge.seek :clear @00:14:06.28 Carousel.seek 46 @00:14:08.47 Lysis1.in :fast @00:14:08.55 Lysis1.seek :locked @00:14:11.20 Plunge.in :default @00:14:11.32 Plunge.seek :locked @00:14:11.48 Air.in :fast @00:14:11.56 Air.seek :locked @00:14:12.57 Carousel.reconfigure :locked @00:14:12.79 Powering cartridge bus @00:14:15.46 Gate.setIR 1750,5000,0,6 @00:14:15.59 Gate.setIR 0,5000,0,6 @00:14:15.68 PuckHeater.configure :default #ambient 25.8C @00:14:15.77 Gate.power :heat,:ON,limit to 5000mA @00:14:16.96 Mounted lysis Cartridge board#Q05046 @00:14:17.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:14:17.17 PhytoSampler with {source: :sampler} @00:14:17.19 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 46" @00:14:17.58 Preparing to filter cartridge 46 @00:14:17.88 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @00:14:23.16 upsync @00:15:03.51 Intake Head Pressure tare is 3% of full scale at 26C (drifted 3%) @00:15:03.54 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @00:15:03.67 PhaseSeparator.in :filling @00:15:03.75 PhaseSeparator.hold @00:15:03.91 Gate.power :sampler,:ON @00:15:10.71 Head pressure reached 1.89psi in 8 seconds @00:15:10.74 Air.in :default @00:15:10.92 Air.seek :sealed @00:15:10.96 Priming sample loop w/150ml, bypass w/50ml @00:15:11.03 SamplePump.setPosition! 0ml @00:15:11.15 Bypass.open @00:15:12.62 Intake.open @00:15:14.04 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:15:16.69 Vacuum tare is 52% of full scale at 26C (drifted 1%) @00:15:16.88 Abs.in :default @00:15:16.97 Abs.hold @00:15:17.07 PhaseSeparator.in :full @00:15:17.17 PhaseSeparator.hold @00:15:17.45 Waiting for sampler to finish priming... @00:15:17.47 delayUntil "00:16:33.94" @00:16:34.01 SamplePump.in :start @00:16:34.10 SamplePump.seek 50ml,3:50 @00:16:34.70 SamplePump.configure :prime @00:17:35.47 Abs.stop @00:17:35.57 PhaseSeparator.stop @00:17:35.65 SamplePump.setPosition! 0ml @00:17:35.73 Exhaust.open @00:17:37.12 Bypass.close @00:17:38.68 Abs.in :default @00:17:38.78 Abs.hold @00:17:38.87 PhaseSeparator.hold @00:17:39.24 SamplePump.in :start @00:17:39.34 SamplePump.seek 150ml,10:30 @00:17:39.93 SamplePump.configure :prime @00:20:42.75 Abs.stop @00:20:42.85 PhaseSeparator.stop @00:20:42.94 Bypass.open @00:20:43.62 Intake.close @00:20:44.42 Toroid.in :hold @00:20:44.51 Toroid.seek :crack @00:20:46.08 Toroid.seek :cartridge @00:20:47.23 Toroid.in :default @00:20:47.31 Toroid.seek :clear @00:20:49.39 Pressurizing cartridge to 26psi above 13.8psia @00:20:49.41 SamplePump.setPosition! 0ml @00:20:49.50 Intake.open @00:20:50.22 Bypass.close @00:20:50.89 Exhaust.close @00:20:51.68 Abs.in :default @00:20:51.75 Abs.hold @00:20:51.87 PhaseSeparator.hold @00:20:52.63 SamplePump.in :charge @00:20:52.85 SamplePump.seek 2.3ml,42 seconds @00:21:07.41 Abs.stop @00:21:07.50 PhaseSeparator.stop @00:21:07.60 Intake.close @00:21:08.38 Pressurized cartridge with 0.952ml to 26.5psi @00:21:08.40 Isolated @36.2psia (22.5psi) @00:21:08.44 Verifying cartridge holds at least 0.65..0.85 of 36.2psia @00:21:08.49 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @00:21:13.11 Cartridge pressure fell 5% to 34.3psia @00:21:13.24 Sampling 25.0ml @00:21:13.26 SamplePump.setPosition! 0ml @00:21:13.36 Exhaust.open @00:21:14.06 Intake.open @00:21:14.97 Abs.in :default @00:21:15.07 Abs.hold @00:21:15.17 PhaseSeparator.hold @00:21:15.55 SamplePump.in :start @00:21:15.66 SamplePump.seek 25ml,2:35 @00:21:16.25 SamplePump.configure :sample @00:21:18.68 Cartridge 46 filtering 2/25ml at 0.65ml/s with 19.7psi vacuum @00:21:55.70 Abs.stop @00:21:55.79 PhaseSeparator.stop @00:21:55.95 Intake.close @00:21:56.03 Exhaust.close @00:21:56.33 Toroid.in :hold @00:21:56.42 Toroid.seek :bypass @00:21:58.09 Toroid.in :default @00:21:58.17 Toroid.seek :clear @00:21:59.82 Sampled 25.0ml @00:22:00.29 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 46" @00:22:00.86 SPR.command "stats_on" @00:22:01.94 SPR.command "start 0 1" @00:22:02.65 SPR.start /var/log/esp/spr24aug0600h.spr.bz2 @00:22:02.99 Gate.power :sampler,:OFF @00:22:04.54 Handoff.select :waste @00:22:04.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:22:05.96 upsync @00:22:11.40 Syringe tare is 52% of full scale at 26C (drifted 1%) @00:22:11.45 PinPort.in :locking @00:22:11.53 PinPort.seek :locked @00:22:12.09 Archive.in :fast @00:22:12.23 Lysis2.in :fast @00:22:12.28 Lysis1.seek 1350uL @00:22:12.32 Archive.seek :full @00:22:12.50 Lysis2.seek 950uL @00:22:17.44 Plunge.seek :reagent @00:22:18.39 Evacuating puck with filter bubble point of 24psi @00:22:18.42 PinPort.seek :locked @00:22:18.55 Handoff.select :waste @00:22:18.61 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:22:25.22 Air syringe pumping up to 1 strokes to reach 8psi @00:22:25.23 Air stroke #1 of 1 @00:22:25.37 Air.in :pressurize @00:22:26.07 Air.seek :pressurized @00:22:31.71 0.8psi after pressurize @00:22:31.74 Air.in :compress @00:22:31.85 Air.seek :evac @00:22:34.89 1.7psi after compress @00:22:53.01 0.7psi after compress (stable) @00:22:53.03 Air.in :default @00:22:53.13 Air.hold @00:22:53.44 at 0.3psi Opened to waste @00:22:53.45 Handoff.select :blocked @00:22:53.64 Archive syringe pumping up to 8 strokes to reach 24psi @00:22:53.65 Archive stroke #1 of 8 @00:22:53.78 Archive.in :pressurize @00:22:53.88 Archive.seek :pressurized @00:23:06.34 1.5psi after pressurize @00:23:21.93 3.1psi after pressurize (stable) @00:23:21.97 Archive.in :compress @00:23:22.12 Archive.seek :empty @00:23:37.93 6.4psi after compress @00:23:51.52 9.2psi after compress (stable) @00:23:51.56 Archive.in :gulp @00:23:51.67 Archive.seek :topVent @00:23:58.01 9.8psi after gulp @00:24:03.62 9.3psi after gulp (stable) @00:24:03.63 Archive stroke #2 of 8 @00:24:03.77 Archive.in :pressurize @00:24:03.85 Archive.seek :pressurized @00:24:16.32 9.9psi after pressurize @00:24:21.91 9.3psi after pressurize (stable) @00:24:21.94 Archive.in :compress @00:24:22.04 Archive.seek :empty @00:24:37.79 23.5psi after compress @00:24:48.38 22.4psi after compress (stable) @00:24:48.42 Archive.in :gulp @00:24:48.52 Archive.seek :topVent @00:24:54.85 23.3psi after gulp @00:25:00.81 22.5psi after gulp (stable) @00:25:00.83 Archive stroke #3 of 8 @00:25:01.00 Archive.in :pressurize @00:25:01.10 Archive.seek :pressurized @00:25:13.56 23.1psi after pressurize @00:25:19.16 22.6psi after pressurize (stable) @00:25:19.19 Archive.in :compress @00:25:19.30 Archive.seek :empty @00:25:29.55 Reached 23.5 of 24.0psi after pumping 5072uL (2.93 strokes) @00:25:29.57 Archive.in :default @00:25:29.72 Archive.hold @00:25:29.82 Handoff.select :waste @00:25:30.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:25:47.65 delay 1:00 @00:26:47.67 Handoff.select :blocked @00:26:47.84 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @00:27:10.44 Handoff.select :waste @00:27:10.62 0.8psi at evacEnd @00:27:10.63 Handoff.select :blocked @00:27:10.84 Archive.in :fast @00:27:10.92 Archive.seek :topVent @00:27:17.72 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @00:27:24.31 Stable @0.3psi after Handoff blocked @00:27:24.34 Archive syringe pumping up to 8 strokes to reach 24psi @00:27:24.36 Archive stroke #1 of 8 @00:27:24.51 Archive.in :pressurize @00:27:24.60 Archive.seek :pressurized @00:27:37.13 0.8psi after pressurize @00:27:37.16 Archive.in :compress @00:27:37.26 Archive.seek :empty @00:27:53.01 15.0psi after compress @00:28:03.64 14.4psi after compress (stable) @00:28:03.68 Archive.in :gulp @00:28:03.78 Archive.seek :topVent @00:28:10.17 15.4psi after gulp @00:28:15.82 14.7psi after gulp (stable) @00:28:15.85 Archive stroke #2 of 8 @00:28:15.99 Archive.in :pressurize @00:28:16.09 Archive.seek :pressurized @00:28:28.56 15.2psi after pressurize @00:28:28.59 Archive.in :compress @00:28:28.74 Archive.seek :empty @00:28:41.78 Reached 24.3 of 24.3psi after pumping 3402uL (1.97 strokes) @00:28:41.81 Archive.in :default @00:28:41.91 Archive.hold @00:28:42.02 Handoff.select :waste @00:28:42.18 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:28:49.28 delay 1:00 @00:29:49.31 Handoff.select :blocked @00:29:49.55 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @00:30:12.20 Handoff.select :waste @00:30:12.35 0.6psi at evacEnd @00:30:12.38 Air.seek :fullVent @00:30:13.75 Archive.in :fast @00:30:13.84 Archive.seek :topVent @00:30:20.82 Seawater evacuated, Pressure above/below filter released. @00:30:20.95 Lysis1.in :slow @00:30:21.04 Lysis1.seek 1050uL @00:30:50.71 delay 5 seconds @00:30:55.73 Handoff.select :blocked @00:30:55.97 Lysis1.seek 450uL @00:31:55.16 delay 3 seconds @00:31:58.29 Air.in mix with 1.57127 parts Lysis1 @00:31:59.09 Air.seek :evac @00:31:59.23 Lysis1.seek 300uL @00:32:14.16 Lysis1.seek :empty @00:32:43.90 Lysis buffer delivered @22.3psi @00:32:43.92 Polling Can every 2:00 @00:32:44.50 PuckHeater.configure :default #ambient 26.5C @00:32:44.58 PuckHeater.seek 79.6C @00:33:39.19 PuckHeater.hold 2:00,79.6C @00:35:39.07 Polling Can every 2:00 @00:35:39.62 PuckHeater.configure :default #ambient 26.5C @00:35:39.72 PuckHeater.hold 30 seconds,78.5C @00:36:09.87 Polling Can every 2:00 @00:36:10.42 PuckHeater.configure :default #ambient 26.5C @00:36:10.51 PuckHeater.hold 30 seconds,78C @00:36:40.72 Polling Can every 2:00 @00:36:41.27 PuckHeater.configure :default #ambient 26.5C @00:36:41.36 PuckHeater.hold 10:00,77.5C @00:46:40.18 Lysis complete. Syringe pressure @31.4psi @00:46:40.22 Archive syringe pumping up to 5 strokes to reach 40psi @00:46:40.25 Archive stroke #1 of 5 @00:46:40.38 Archive.in :pressurize @00:46:40.48 Archive.seek :pressurized @00:46:52.98 31.0psi after pressurize @00:46:58.57 30.4psi after pressurize (stable) @00:46:58.59 Archive.in :compress @00:46:58.74 Archive.seek :empty @00:47:08.30 Reached 40.9 of 40.0psi after pumping 1597uL (0.92 strokes) @00:47:08.33 Archive.in :default @00:47:08.42 Archive.hold @00:47:08.53 delay 2 seconds @00:47:10.56 Archive.in :fast @00:47:10.67 Archive.seek :full @00:47:17.50 Lysis1.seek 138uL @00:47:31.22 delay 1 second @00:47:32.26 Archive.seek :full @00:47:32.30 Lysis1.seek :empty @00:47:46.08 Handoff.select :blocked @00:47:46.22 Initial air pressure @48.1psi @00:47:46.32 Lysis1.in :extract @00:47:46.45 Lysis1.seek 600uL,nil @00:47:48.76 Archive syringe pumping up to 6 strokes to reach 25psi @00:47:48.78 Archive stroke #1 of 6 @00:47:48.93 Archive.in :pressurize @00:47:49.04 Archive.seek :pressurized @00:48:01.55 15.2psi after pressurize @00:48:07.15 14.7psi after pressurize (stable) @00:48:07.20 Archive.in :compress @00:48:07.28 Archive.seek :empty @00:48:23.04 16.4psi after compress @00:48:33.63 14.9psi after compress (stable) @00:48:38.85 Recovering 2.7uL/s @00:48:44.01 Archive.in :gulp @00:48:44.09 Archive.seek :topVent @00:48:51.11 15.4psi after gulp @00:48:56.83 15.0psi after gulp (stable) @00:48:56.84 Archive stroke #2 of 6 @00:48:57.00 Archive.in :pressurize @00:48:57.12 Archive.seek :pressurized @00:49:09.70 15.1psi after pressurize @00:49:15.30 14.5psi after pressurize (stable) @00:49:15.34 Archive.in :compress @00:49:15.49 Archive.seek :empty @00:49:25.23 Archive.in :default @00:49:25.30 Archive.hold @00:49:25.50 Initial 600uL lysate @15.7psi @00:49:25.52 Handoff.select :blocked @00:49:25.67 Initial air pressure @15.2psi @00:49:25.75 Lysis1.seek 825uL,nil @00:49:25.92 Archive syringe pumping up to 6 strokes to reach 17psi @00:49:25.93 Archive stroke #1 of 6 @00:49:26.17 Archive.in :compress @00:49:26.29 Archive.seek :empty @00:49:26.68 Reached 17.3 of 17.0psi after pumping 1604uL (0.93 strokes) @00:49:26.78 Archive.in :default @00:49:26.91 Archive.hold @00:49:27.32 Archive syringe pumping up to 6 strokes to reach 17psi @00:49:27.34 Archive stroke #1 of 6 @00:49:27.55 Archive.in :compress @00:49:27.66 Archive.seek :empty @00:49:30.82 Archive.in :default @00:49:30.89 Archive.hold @00:49:31.90 Recovered 825uL lysate @17.4psi @00:49:31.96 Air.in :halfSpeed @00:49:32.08 Air.seek :fullVent @00:49:34.14 Puck Top Vented, bottom @10.6psi @00:49:34.15 Handoff.select :waste @00:49:34.33 delay 5 seconds @00:49:39.41 Handoff open to waste, bottom @0.0psi @00:49:39.44 Air.in :default @00:49:39.54 Air.seek :evac @00:49:40.89 Lysis2.in :DAmix @00:49:41.05 Lysis1.in :DAmix @00:49:41.79 Lysis2.seek 700uL @00:49:41.84 Lysis1.seek 575uL @00:50:16.46 delay 5 seconds @00:50:21.49 Handoff.select SPR @00:50:21.65 SPR.command "start 2 14" @00:50:22.27 SPR stabilized @00:50:22.47 delay 10 seconds @00:50:32.49 SPR.command "start 2 0" @00:50:35.65 Waiting up to 11:00 for SPR to enter state 1 @00:52:33.31 SPR.command "hold_on" @00:52:34.71 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:52:35.52 Lysis2.seek 400uL @00:52:35.57 Lysis1.seek 275uL @00:53:17.10 delay 15 seconds @00:53:32.12 SPR.command "hold_off" @00:53:35.36 Waiting up to 1:00:00 for SPR to enter state 7 @01:09:50.93 SPRsummary:-0.179RIU,49.1RIU,48.5RIU,0.863RIU,743ng/L,781ng/L,high after 10094s @01:24:07.83 SPR analysis completed @01:24:17.81 Ended log spr24aug0600h.spr.bz2 @01:24:17.83 SPR.command "stats_off" @01:24:18.87 Handoff.select :close @01:24:19.05 Gate.power :heat,:OFF @01:24:19.07 Gate.power :cartridge,:OFF @01:24:19.19 PinPort.in :unlocking @01:24:19.27 PinPort.seek :clear @01:24:19.78 Carousel.in :default @01:24:19.92 Carousel.home.seek 46 @01:24:20.29 Plunge.seek :locked @01:24:21.21 Archive.in :unlocking @01:24:21.36 Archive.seek :clear @01:24:21.49 Air.in :unlocking @01:24:21.64 Air.seek :clear @01:24:21.77 Lysis1.in :unlocking @01:24:21.93 Lysis1.seek :clear @01:24:22.63 Lysis2.in :unlocking @01:24:22.75 Lysis2.seek :clear @01:24:26.61 Gate.power :analytic1,:OFF @01:24:26.63 Gate.power :motor,:OFF @01:24:26.71 Gate.power :core,:OFF @01:24:26.74 Gate.power :main,:OFF @01:24:26.78 Safely stopped and ready to power off @02:06:14.06 -> exit @02:06:14.09 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @12:04:37.32 Session Begun @12:04:40.48 -> can Can@12:04:37, 22.9C, 49% humidity, 14.4psia, 14.877V, 0.088A, 9.526Ah, 1.32W @12:13:57.72 -> can Can@12:13:57, 23.2C, 48% humidity, 14.4psia, 14.877V, 0.088A, 9.539Ah, 1.31W @12:14:55.73 -> exit @12:14:55.75 Session Ended @13:44:23.20 Session Begun @13:44:26.54 -> can Can@13:44:23, 24.4C, 47% humidity, 14.4psia, 14.872V, 0.088A, 9.661Ah, 1.31W @13:44:31.89 -> exit @13:44:31.91 Session Ended @13:53:49.74 Session Begun @13:53:53.36 -> can Can@13:53:50, 24.5C, 47% humidity, 14.4psia, 14.873V, 0.088A, 9.673Ah, 1.31W @13:54:15.48 -> exit @13:54:15.51 Session Ended @16:04:08.35 Session Begun @16:04:11.25 -> can Can@16:04:08, 25.3C, 47% humidity, 14.4psia, 14.873V, 0.088A, 9.849Ah, 1.31W @17:55:53.42 -> can Can@17:55:53, 25.8C, 47% humidity, 14.4psia, 14.874V, 0.088A, 10.000Ah, 1.31W @17:56:59.48 -> exit @17:56:59.50 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @17:57:13.50 Session Begun @17:57:32.90 -> ESP.ready! @17:57:32.96 Gate.power :sampler,:OFF,limit to 3500mA @17:57:32.98 Gate.power :skimmer,:OFF,limit to 3500mA @17:57:33.01 Gate.power :main,:ON,limit to 5000mA @17:57:33.05 Gate.power :analytic1,:ON,limit to 4000mA @17:57:33.14 Powering core bus @17:57:35.50 SPR Module 1, FW Rev. B, FPGA Rev. C @17:57:35.92 Plunge.configure :default @17:57:36.03 SamplePump.configure :default @17:57:36.14 Toroid.configure :default @17:57:36.25 Carousel.configure :default @17:57:36.36 Abs.configure :default @17:57:36.45 PhaseSeparator.configure :full @17:57:36.56 Archive.configure :default @17:57:36.70 Air.configure :default @17:57:36.78 Lysis1.configure :default @17:57:36.86 Lysis2.configure :default @17:57:36.97 PinPort.configure :default @17:57:37.05 All elf controllers running firmware version 4.92 @17:57:37.08 Gate.power :motor,:ON,limit to 5000mA @17:57:37.45 Plunge.home.jog 0 @17:57:38.10 Lysis2.home.jog 0 @17:57:38.17 Lysis1.home.jog 0 @17:57:38.22 Archive.home.jog 0 @17:57:38.26 Toroid.home.jog 0 @17:57:38.34 Air.home.jog 0 @17:57:38.41 PinPort.home.jog 0 @17:57:38.46 Carousel.home.jog 0 @17:57:39.33 Plunge.in :homing @17:57:39.63 Plunge.jog 1604 @17:57:39.87 PinPort.in :homing @17:57:39.92 Lysis2.in :homing @17:57:39.95 Air.in :homing @17:57:40.01 Toroid.in :homing @17:57:40.04 Lysis1.in :homing @17:57:40.12 Archive.in :homing @17:57:40.31 PinPort.jog -107 @17:57:40.38 Lysis2.jog 1407 @17:57:40.50 Air.jog 1372 @17:57:40.59 Toroid.jog 621 @17:57:40.70 Lysis1.jog 1397 @17:57:40.74 Archive.jog 1397 @17:57:41.16 Plunge.setRawPosition! 50 @17:57:41.19 PinPort.setRawPosition! 0 @17:57:41.33 Plunge.seek :home @17:57:41.40 PinPort.seek :home @17:57:41.58 PinPort.in :default @17:57:41.72 Plunge.in :default @17:57:41.98 Lysis2.setRawPosition! 50 @17:57:42.64 Lysis1.setRawPosition! 50 @17:57:42.72 Air.setRawPosition! 50 @17:57:42.76 Lysis2.seek :home @17:57:42.80 Archive.setRawPosition! 50 @17:57:43.04 Lysis1.seek :home @17:57:43.07 Air.seek :home @17:57:43.33 Archive.seek :home @17:57:43.53 Lysis1.in :default @17:57:43.56 Lysis2.in :default @17:57:43.60 Toroid.setRawPosition! 0 @17:57:43.81 Toroid.seek :home @17:57:43.97 Archive.in :default @17:57:44.01 Toroid.in :default @17:57:44.07 Air.in :default @17:57:44.51 Toroid.seek :clear @17:57:46.29 Carousel.in :default @17:57:46.44 Carousel.home.seek 46 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 46 Abs = Absolute Pressure (Leak) Sensor @14psia PhaseSeparator = Intake Head Pressure @-0.08psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:58:14.84 -> deep; fire archive, lysis # @17:58:15.83 Toroid.seek :clear @17:58:16.03 Carousel.in :default @17:58:16.17 Carousel.home.seek 46 @17:58:16.46 Selecting Cartridge 51 @17:58:16.82 Carousel.seek 51 @17:58:18.19 Lysis1.in :fast @17:58:18.28 Lysis1.seek :locked @17:58:20.93 Plunge.seek :locked @17:58:21.12 Air.in :fast @17:58:21.21 Air.seek :locked @17:58:22.22 Carousel.reconfigure :locked @17:58:22.40 Powering cartridge bus @17:58:25.08 Gate.setIR 1750,5000,0,6 @17:58:25.21 Gate.setIR 0,5000,0,6 @17:58:25.73 Gate.power :cartridge,:OFF @17:58:26.41 Mounted archive Cartridge @17:58:26.49 BACsampler with {source: :sampler} @17:58:26.51 GoodNews.email "BACsampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 51" @17:58:26.93 Preparing to filter cartridge 51 @17:58:27.23 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @17:58:32.71 upsync @17:58:49.81 Intake Head Pressure tare is 3% of full scale at 26C (drifted 2%) @17:58:49.93 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @17:58:49.99 PhaseSeparator.in :filling @17:58:50.07 PhaseSeparator.hold @17:58:50.16 Gate.power :sampler,:ON @17:58:57.01 Head pressure reached 2.08psi in 8 seconds @17:58:57.12 Priming sample loop w/150ml, bypass w/50ml @17:58:57.15 SamplePump.setPosition! 0ml @17:58:57.24 Bypass.open @17:58:58.73 Intake.open @17:58:59.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:59:02.17 Vacuum tare is 52% of full scale at 26C (drifted 0%) @17:59:02.36 Abs.in :default @17:59:02.44 Abs.hold @17:59:02.56 PhaseSeparator.in :full @17:59:02.63 PhaseSeparator.hold @17:59:02.94 Waiting for sampler to finish priming... @17:59:02.95 delayUntil "18:00:20.18" @18:00:20.27 SamplePump.in :start @18:00:20.41 SamplePump.seek 50ml,3:50 @18:00:20.96 SamplePump.configure :prime @18:01:21.81 Abs.stop @18:01:21.89 PhaseSeparator.stop @18:01:21.98 SamplePump.setPosition! 0ml @18:01:22.05 Exhaust.open @18:01:23.47 Bypass.close @18:01:25.02 Abs.in :default @18:01:25.11 Abs.hold @18:01:25.21 PhaseSeparator.hold @18:01:25.57 SamplePump.in :start @18:01:25.67 SamplePump.seek 150ml,10:30 @18:01:26.26 SamplePump.configure :prime @18:04:11.21 -> exit @18:04:11.23 Session Ended @18:04:29.10 Abs.stop @18:04:29.16 PhaseSeparator.stop @18:04:29.24 Bypass.open @18:04:29.93 Intake.close @18:04:30.74 Toroid.in :hold @18:04:30.80 Toroid.seek :crack @18:04:32.35 Toroid.seek :cartridge @18:04:33.46 Toroid.in :default @18:04:33.52 Toroid.seek :clear @18:04:36.09 Pressurizing cartridge to 26psi above 14.4psia @18:04:36.10 SamplePump.setPosition! 0ml @18:04:36.15 Intake.open @18:04:36.84 Bypass.close @18:04:37.52 Exhaust.close @18:04:38.30 Abs.in :default @18:04:38.35 Abs.hold @18:04:38.44 PhaseSeparator.hold @18:04:38.73 SamplePump.in :charge @18:04:38.79 SamplePump.seek 2.3ml,42 seconds @18:04:46.94 Abs.stop @18:04:46.99 PhaseSeparator.stop @18:04:47.05 Intake.close @18:04:47.79 Pressurized cartridge with 0.297ml to 27.2psi @18:04:47.80 Isolated @36.7psia (22.3psi) @18:04:47.82 Verifying cartridge holds at least 0.65..0.85 of 36.7psia @18:04:47.84 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:04:52.44 Cartridge pressure fell 3% to 35.6psia @18:04:52.53 Sampling 1000.0ml @18:04:52.55 SamplePump.setPosition! 0ml @18:04:52.60 Exhaust.open @18:04:53.27 Intake.open @18:04:54.14 Abs.in :default @18:04:54.19 Abs.hold @18:04:54.27 PhaseSeparator.hold @18:04:54.60 SamplePump.in :start @18:04:54.70 SamplePump.seek 1000ml,1:23:50 @18:04:55.29 SamplePump.configure :sample @18:04:57.70 Cartridge 51 filtering 2/1000ml at 0.72ml/s with 17.7psi vacuum @18:05:57.75 Cartridge 51 filtering 35/1000ml at 0.56ml/s with 32.9psi vacuum @18:06:57.76 Cartridge 51 filtering 66/1000ml at 0.52ml/s with 24.9psi vacuum @18:07:57.76 Cartridge 51 filtering 96/1000ml at 0.49ml/s with 23.8psi vacuum @18:08:57.76 Cartridge 51 filtering 124/1000ml at 0.46ml/s with 24.7psi vacuum @18:09:57.76 Cartridge 51 filtering 149/1000ml at 0.43ml/s with 19.8psi vacuum @18:10:57.75 Cartridge 51 filtering 173/1000ml at 0.39ml/s with 23.0psi vacuum @18:11:57.77 Cartridge 51 filtering 194/1000ml at 0.35ml/s with 21.3psi vacuum 4.3m deep (21.1psia) @18:12:58.21 Cartridge 51 filtering 213/1000ml at 0.31ml/s with 21.8psi vacuum @18:13:36.70 Session Begun @18:13:40.32 -> showlog @18:13:57.76 Cartridge 51 filtering 229/1000ml at 0.27ml/s with 21.8psi vacuum @18:14:57.76 Cartridge 51 filtering 243/1000ml at 0.23ml/s with 24.7psi vacuum @18:15:57.76 Cartridge 51 filtering 255/1000ml at 0.20ml/s with 28.3psi vacuum @18:15:57.84 SamplePump.stop @18:15:57.95 Intake.close @18:15:58.01 Exhaust.close @18:15:58.33 Toroid.in :hold @18:15:58.42 Toroid.seek :bypass @18:16:00.10 Toroid.in :default @18:16:00.20 Toroid.seek :clear @18:16:01.86 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @18:16:01.89 Abs.stop @18:16:01.99 PhaseSeparator.stop @18:16:02.31 Sampled 254.6ml @18:16:02.82 GoodNews.email "After filtering 254.6ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 51" @18:16:03.42 Plunge.seek :reagent @18:16:04.28 Sample loop closed. Ready to Deliver Reagents @18:16:04.31 Archive.seek :sealed @18:16:05.48 Gate.power :sampler,:OFF @18:16:08.33 Archive.seek :evac @18:16:08.70 upsync @18:16:22.61 delay 1:00 @18:17:22.67 Archive.seek :clear @18:17:40.41 RNAlater soak for 10:00 @18:27:40.45 Archive.seek :tVent @18:27:43.01 delay 1 second @18:27:44.04 Archive.seek :evac @18:27:59.71 delay 10 seconds @18:28:09.75 Archive.seek :tVent @18:28:25.43 delay 1 second @18:28:26.46 Archive.seek :evac @18:28:42.13 delay 10 seconds @18:28:52.17 Sample preserved and evacuated @18:28:52.36 Selecting Cartridge 44 @18:28:52.62 Carousel.in :default @18:28:53.31 Carousel.home.seek 51 @18:28:53.76 Archive.in :unlocking @18:28:53.91 Archive.seek :clear @18:28:54.05 Air.in :unlocking @18:28:54.22 Air.seek :clear @18:28:58.47 Lysis1.in :unlocking @18:28:58.64 Lysis1.seek :clear @18:28:58.74 Plunge.in :unlocking @18:28:58.89 Plunge.seek :clear @18:29:00.31 Carousel.seek 44 @18:29:02.12 Lysis1.in :fast @18:29:02.21 Lysis1.seek :locked @18:29:04.86 Plunge.in :default @18:29:04.93 Plunge.seek :locked @18:29:05.12 Air.in :fast @18:29:05.21 Air.seek :locked @18:29:06.22 Carousel.reconfigure :locked @18:29:06.42 Powering cartridge bus @18:29:09.09 Gate.setIR 1750,5000,0,6 @18:29:09.22 Gate.setIR 0,5000,0,6 @18:29:09.31 PuckHeater.configure :default #ambient 26.5C @18:29:09.40 Gate.power :heat,:ON,limit to 5000mA @18:29:10.11 Mounted lysis Cartridge board#Q05065 @18:29:10.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:29:10.24 PhytoSampler with {source: :sampler} @18:29:10.26 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 44" @18:29:10.69 Preparing to filter cartridge 44 @18:29:11.04 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @18:29:15.67 upsync @18:29:21.18 Intake Head Pressure tare is 3% of full scale at 27C (drifted 2%) @18:29:21.20 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @18:29:21.27 PhaseSeparator.in :filling @18:29:21.35 PhaseSeparator.hold @18:29:21.46 Gate.power :sampler,:ON @18:29:28.32 Head pressure reached 1.92psi in 8 seconds @18:29:28.37 Air.in :default @18:29:28.55 Priming sample loop w/150ml, bypass w/50ml @18:29:28.57 Air.seek :sealed @18:29:28.62 SamplePump.setPosition! 0ml @18:29:28.81 Bypass.open @18:29:30.28 Intake.open @18:29:31.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:29:33.67 Vacuum tare is 52% of full scale at 27C (drifted 0%) @18:29:33.86 Abs.in :default @18:29:33.94 Abs.hold @18:29:34.05 PhaseSeparator.in :full @18:29:34.13 PhaseSeparator.hold @18:29:34.38 Waiting for sampler to finish priming... @18:29:34.39 delayUntil "18:30:51.48" @18:30:51.55 SamplePump.in :start @18:30:51.65 SamplePump.seek 50ml,3:50 @18:30:52.24 SamplePump.configure :prime @18:31:53.03 Abs.stop @18:31:53.12 PhaseSeparator.stop @18:31:53.20 SamplePump.setPosition! 0ml @18:31:53.27 Exhaust.open @18:31:54.63 Bypass.close @18:31:56.22 Abs.in :default @18:31:56.31 Abs.hold @18:31:56.41 PhaseSeparator.hold @18:31:56.81 SamplePump.in :start @18:31:56.91 SamplePump.seek 150ml,10:30 @18:31:57.51 SamplePump.configure :prime @18:35:00.61 Abs.stop @18:35:01.28 PhaseSeparator.stop @18:35:01.42 Bypass.open @18:35:02.15 Intake.close @18:35:02.97 Toroid.in :hold @18:35:03.06 Toroid.seek :crack @18:35:04.63 Toroid.seek :cartridge @18:35:05.82 Toroid.in :default @18:35:05.90 Toroid.seek :clear @18:35:07.96 Pressurizing cartridge to 26psi above 14.5psia @18:35:07.99 SamplePump.setPosition! 0ml @18:35:08.07 Intake.open @18:35:08.79 Bypass.close @18:35:09.51 Exhaust.close @18:35:10.32 Abs.in :default @18:35:10.40 Abs.hold @18:35:10.50 PhaseSeparator.hold @18:35:10.82 SamplePump.in :charge @18:35:10.93 SamplePump.seek 2.3ml,42 seconds @18:35:24.63 Abs.stop @18:35:24.77 PhaseSeparator.stop @18:35:24.85 Intake.close @18:35:25.65 Pressurized cartridge with 0.843ml to 27.4psi @18:35:25.66 Isolated @36.5psia (22.0psi) @18:35:25.71 Verifying cartridge holds at least 0.65..0.85 of 36.5psia @18:35:25.77 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:35:30.38 Cartridge pressure fell 4% to 35.2psia @18:35:30.50 Sampling 25.0ml @18:35:30.52 SamplePump.setPosition! 0ml @18:35:30.61 Exhaust.open @18:35:31.36 Intake.open @18:35:32.25 Abs.in :default @18:35:32.32 Abs.hold @18:35:32.43 PhaseSeparator.hold @18:35:32.85 SamplePump.in :start @18:35:32.96 SamplePump.seek 25ml,2:35 @18:35:33.54 SamplePump.configure :sample @18:35:35.94 Cartridge 44 filtering 2/25ml at 0.61ml/s with 20.1psi vacuum @18:36:13.38 Abs.stop @18:36:13.55 PhaseSeparator.stop @18:36:13.71 Intake.close @18:36:13.79 Exhaust.close @18:36:14.10 Toroid.in :hold @18:36:14.20 Toroid.seek :bypass @18:36:15.86 Toroid.in :default @18:36:15.95 Toroid.seek :clear @18:36:17.60 Sampled 25.0ml @18:36:18.13 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 44" @18:36:19.78 SPR.command "read_sequences" @18:36:20.24 SPR.command "stats_on" @18:36:20.44 SPR.command "start 0 1" @18:36:20.77 Gate.power :sampler,:OFF @18:36:21.14 SPR.start /var/log/esp/spr24aug0618h.spr.bz2 @18:36:23.10 Handoff.select :waste @18:36:23.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:36:24.34 upsync @18:36:29.93 Syringe tare is 51% of full scale at 27C (drifted 0%) @18:36:29.98 PinPort.in :locking @18:36:30.07 PinPort.seek :locked @18:36:30.59 Archive.in :fast @18:36:30.74 Lysis2.in :fast @18:36:30.84 Lysis1.seek 1350uL @18:36:30.92 Archive.seek :full @18:36:31.13 Lysis2.seek 950uL @18:36:35.97 Plunge.seek :reagent @18:36:36.96 Evacuating puck with filter bubble point of 24psi @18:36:36.99 PinPort.seek :locked @18:36:37.15 Handoff.select :waste @18:36:37.20 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:36:43.81 Air syringe pumping up to 1 strokes to reach 8psi @18:36:43.82 Air stroke #1 of 1 @18:36:43.96 Air.in :pressurize @18:36:44.04 Air.seek :pressurized @18:36:49.69 0.8psi after pressurize @18:36:49.71 Air.in :compress @18:36:49.82 Air.seek :evac @18:36:52.89 1.3psi after compress @18:37:03.51 0.2psi after compress (stable) @18:37:03.52 Air.in :default @18:37:03.67 Air.hold @18:37:04.58 at 0.2psi Opened to waste @18:37:04.60 Handoff.select :blocked @18:37:04.81 Archive syringe pumping up to 8 strokes to reach 24psi @18:37:04.83 Archive stroke #1 of 8 @18:37:04.98 Archive.in :pressurize @18:37:05.06 Archive.seek :pressurized @18:37:17.55 8.7psi after pressurize @18:37:23.17 8.4psi after pressurize (stable) @18:37:23.22 Archive.in :compress @18:37:23.33 Archive.seek :empty @18:37:39.08 19.0psi after compress @18:37:54.18 21.1psi after compress (stable) @18:37:54.21 Archive.in :gulp @18:37:54.32 Archive.seek :topVent @18:38:00.74 22.4psi after gulp @18:38:06.34 21.8psi after gulp (stable) @18:38:06.35 Archive stroke #2 of 8 @18:38:06.49 Archive.in :pressurize @18:38:06.58 Archive.seek :pressurized @18:38:19.07 21.9psi after pressurize @18:38:26.19 Reached 24.0 of 24.0psi after pumping 3127uL (1.81 strokes) @18:38:26.22 Archive.in :default @18:38:26.32 Archive.hold @18:38:26.43 Handoff.select :waste @18:38:26.61 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:38:44.74 delay 1:00 @18:39:44.77 Handoff.select :blocked @18:39:44.94 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @18:40:07.54 Handoff.select :waste @18:40:07.73 0.7psi at evacEnd @18:40:07.77 Handoff.select :blocked @18:40:07.96 Archive.in :fast @18:40:08.04 Archive.seek :topVent @18:40:14.08 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @18:40:20.71 Stable @0.5psi after Handoff blocked @18:40:20.73 Archive syringe pumping up to 8 strokes to reach 24psi @18:40:20.76 Archive stroke #1 of 8 @18:40:20.91 Archive.in :pressurize @18:40:21.01 Archive.seek :pressurized @18:40:33.49 8.0psi after pressurize @18:40:33.52 Archive.in :compress @18:40:33.63 Archive.seek :empty @18:40:49.42 14.0psi after compress @18:41:00.02 12.7psi after compress (stable) @18:41:00.05 Archive.in :gulp @18:41:00.16 Archive.seek :topVent @18:41:06.51 13.5psi after gulp @18:41:12.14 12.7psi after gulp (stable) @18:41:12.16 Archive stroke #2 of 8 @18:41:12.31 Archive.in :pressurize @18:41:12.40 Archive.seek :pressurized @18:41:24.86 13.5psi after pressurize @18:41:24.89 Archive.in :compress @18:41:25.00 Archive.seek :empty @18:41:38.26 Reached 24.6 of 24.5psi after pumping 3406uL (1.97 strokes) @18:41:38.29 Archive.in :default @18:41:38.39 Archive.hold @18:41:38.49 Handoff.select :waste @18:41:38.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:41:51.87 delay 1:00 @18:42:51.89 Handoff.select :blocked @18:42:52.06 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @18:43:14.69 Handoff.select :waste @18:43:15.32 0.9psi at evacEnd @18:43:15.44 Air.seek :fullVent @18:43:16.81 Archive.in :fast @18:43:16.93 Archive.seek :topVent @18:43:23.96 Seawater evacuated, Pressure above/below filter released. @18:43:24.09 Lysis1.in :slow @18:43:24.18 Lysis1.seek 1050uL @18:43:53.84 delay 5 seconds @18:43:58.86 Handoff.select :blocked @18:43:59.09 Lysis1.seek 450uL @18:44:58.28 delay 3 seconds @18:45:01.38 Air.in mix with 1.57127 parts Lysis1 @18:45:02.64 Air.seek :evac @18:45:02.80 Lysis1.seek 300uL @18:45:17.77 Lysis1.seek :empty @18:45:47.50 Lysis buffer delivered @6.5psi @18:45:47.51 Polling Can every 2:00 @18:45:48.07 PuckHeater.configure :default #ambient 27.5C @18:45:48.15 PuckHeater.seek 79.5C @18:46:34.14 PuckHeater.hold 2:00,79.5C @18:48:33.91 Polling Can every 2:00 @18:48:34.46 PuckHeater.configure :default #ambient 27.6C @18:48:34.55 PuckHeater.hold 30 seconds,78.5C @18:49:04.72 Polling Can every 2:00 @18:49:05.28 PuckHeater.configure :default #ambient 27.6C @18:49:05.37 PuckHeater.hold 30 seconds,78C @18:49:35.53 Polling Can every 2:00 @18:49:36.08 PuckHeater.configure :default #ambient 27.6C @18:49:36.20 PuckHeater.hold 10:00,77.4C @18:59:34.70 Lysis complete. Syringe pressure @9.7psi @18:59:34.75 Archive syringe pumping up to 5 strokes to reach 40psi @18:59:34.77 Archive stroke #1 of 5 @18:59:34.94 Archive.in :pressurize @18:59:35.05 Archive.seek :pressurized @18:59:47.50 10.3psi after pressurize @19:00:01.60 20.3psi after pressurize (stable) @19:00:01.63 Archive.in :compress @19:00:01.74 Archive.seek :empty @19:00:17.53 36.5psi after compress @19:00:28.12 35.3psi after compress (stable) @19:00:28.16 Archive.in :gulp @19:00:28.26 Archive.seek :topVent @19:00:34.61 35.8psi after gulp @19:00:40.26 35.0psi after gulp (stable) @19:00:40.27 Archive stroke #2 of 5 @19:00:40.41 Archive.in :pressurize @19:00:40.49 Archive.seek :pressurized @19:00:52.95 35.3psi after pressurize @19:00:59.05 34.5psi after pressurize (stable) @19:00:59.10 Archive.in :compress @19:00:59.22 Archive.seek :empty @19:01:03.41 Reached 40.4 of 40.0psi after pumping 3212uL (1.86 strokes) @19:01:03.43 Archive.in :default @19:01:03.53 Archive.hold @19:01:03.64 delay 2 seconds @19:01:05.68 Archive.in :fast @19:01:05.80 Archive.seek :full @19:01:12.21 Lysis1.seek 138uL @19:01:25.93 delay 1 second @19:01:26.97 Archive.seek :full @19:01:27.05 Lysis1.seek :empty @19:01:40.82 Handoff.select :blocked @19:01:40.95 Initial air pressure @40.3psi @19:01:41.54 Lysis1.in :extract @19:01:41.81 Lysis1.seek 600uL,nil @19:01:47.42 Initial 600uL lysate @18.9psi @19:01:47.44 Handoff.select :blocked @19:01:47.60 Initial air pressure @18.8psi @19:01:47.72 Lysis1.seek 825uL,nil @19:01:49.63 Archive syringe pumping up to 6 strokes to reach 17psi @19:01:49.64 Archive stroke #1 of 6 @19:01:49.86 Archive.in :pressurize @19:01:49.95 Archive.seek :pressurized @19:01:50.15 Archive.in :default @19:01:50.27 Archive.hold @19:01:50.75 Recovered 826uL lysate @15.3psi @19:01:50.78 Air.in :halfSpeed @19:01:50.87 Air.seek :fullVent @19:01:52.91 Puck Top Vented, bottom @15.5psi @19:01:52.92 Handoff.select :waste @19:01:53.13 delay 5 seconds @19:01:58.21 Handoff open to waste, bottom @0.7psi @19:01:58.24 Air.in :default @19:01:58.35 Air.seek :evac @19:01:59.69 Lysis2.in :DAmix @19:01:59.85 Lysis1.in :DAmix @19:02:01.10 Lysis2.seek 700uL @19:02:01.16 Lysis1.seek 576uL @19:02:35.81 delay 5 seconds @19:02:40.83 Handoff.select SPR @19:02:41.00 SPR.command "start 2 14" @19:02:41.68 SPR stabilized @19:02:41.88 delay 10 seconds @19:02:51.91 SPR.command "start 2 0" @19:02:55.58 Waiting up to 11:00 for SPR to enter state 1 @19:04:53.67 SPR.command "hold_on" @19:04:55.58 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:04:56.31 Lysis2.seek 400uL @19:04:56.37 Lysis1.seek 276uL @19:05:37.92 delay 15 seconds @19:05:52.94 SPR.command "hold_off" @19:05:56.24 Waiting up to 1:00:00 for SPR to enter state 7 @19:22:11.29 SPRsummary:4.26RIU,428RIU,99.1RIU,5.69RIU,none,270ng/L,high after 2905s @19:36:28.17 SPR analysis completed @19:36:37.59 Ended log spr24aug0618h.spr.bz2 @19:36:37.61 SPR.command "stats_off" @19:36:37.66 ThreadError in PROCESSING -- SPRlogger thread died holding # @19:36:38.16 Archive.stop @19:36:38.33 Handoff.select :close @19:36:38.52 Gate.power :heat,:OFF @19:36:38.54 Gate.power :cartridge,:OFF @19:36:38.70 PinPort.in :unlocking @19:36:38.79 PinPort.seek :clear @19:36:39.29 Carousel.in :default @19:36:39.44 Carousel.home.seek 44 @19:36:40.30 Plunge.seek :locked @19:36:41.23 Archive.in :unlocking @19:36:41.39 Archive.seek :clear @19:36:41.55 Air.in :unlocking @19:36:41.71 Air.seek :clear @19:36:41.78 Lysis1.in :unlocking @19:36:41.98 Lysis1.seek :clear @19:36:42.12 Lysis2.in :unlocking @19:36:42.21 Lysis2.seek :clear @19:36:46.16 Gate.power :analytic1,:OFF @19:36:46.19 Gate.power :motor,:OFF @19:36:46.23 Gate.power :core,:OFF @19:36:46.26 Gate.power :main,:OFF @19:36:46.29 Safely stopped and ready to power off @19:38:41.93 -> exit @19:38:41.97 Session Ended @23:15:09.03 Session Begun @23:15:15.78 -> can Can@23:15:09, 23.5C, 51% humidity, 14.4psia, 14.877V, 0.088A, 11.810Ah, 1.30W @23:15:22.98 -> exit @23:15:23.01 Session Ended @11:37:01.38UTC07-Aug-24 Session Begun @11:37:03.72 -> CAn @11:37:03.75 NameError in bill -- uninitialized constant CAn @11:37:05.96 -> Can Can@11:36:45, 26.3C, 49% humidity, 14.4psia, 14.881V, 0.089A, 12.812Ah, 1.32W @11:37:54.21 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 826, spr: spr24aug0618h.spr.bz2}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 825, spr: spr24aug0600h.spr.bz2}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 825, spr: spr24aug0519h.spr.bz2}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, 54..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug0520h.spr.bz2}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0120h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:51:31.34 Session Begun @11:51:47.57 -> exit @11:51:47.59 Session Ended @11:54:43.72 -> espclient bill @11:54:43.77 NoMethodError in bill -- undefined method `espclient' for CmdServer::Client:Class @11:54:55.57 -> esp :esp @11:55:05.81 -> espclient bill @11:55:05.85 NoMethodError in bill -- undefined method `espclient' for CmdServer::Client:Class @11:55:24.70 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [49,47,45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 826, spr: spr24aug0618h.spr.bz2}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 825, spr: spr24aug0600h.spr.bz2}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25.0, lysed: 825, spr: spr24aug0519h.spr.bz2}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, 54..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug0520h.spr.bz2}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0120h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @11:55:37.49 -> PhytoSampler.xray @11:56:24.85 -> PhytoSampler.recoveryVolume=600..780 600..780 @11:56:29.97 -> PhytoSampler.xray @11:56:46.29 -> fire lysis # @11:56:46.54 Gate.power :sampler,:OFF,limit to 3500mA @11:56:46.57 Gate.power :skimmer,:OFF,limit to 3500mA @11:56:46.61 Gate.power :main,:ON,limit to 5000mA @11:56:46.64 Gate.power :analytic1,:ON,limit to 4000mA @11:56:46.79 Powering core bus @11:56:49.13 SPR Module 1, FW Rev. B, FPGA Rev. C @11:56:49.58 Plunge.configure :default @11:56:49.69 SamplePump.configure :default @11:56:49.79 Toroid.configure :default @11:56:49.90 Carousel.configure :default @11:56:50.01 Abs.configure :default @11:56:50.12 PhaseSeparator.configure :full @11:56:50.23 Archive.configure :default @11:56:50.34 Air.configure :default @11:56:50.43 Lysis1.configure :default @11:56:50.50 Lysis2.configure :default @11:56:50.60 PinPort.configure :default @11:56:50.73 All elf controllers running firmware version 4.92 @11:56:50.76 Gate.power :motor,:ON,limit to 5000mA @11:56:51.23 Plunge.home.jog 0 @11:56:51.84 PinPort.home.jog 0 @11:56:51.87 Lysis2.home.jog 0 @11:56:51.93 Air.home.jog 0 @11:56:51.97 Carousel.home.jog 0 @11:56:52.03 Lysis1.home.jog 0 @11:56:52.14 Archive.home.jog 0 @11:56:52.18 Toroid.home.jog 0 @11:56:53.03 Plunge.in :homing @11:56:53.93 Lysis2.in :homing @11:56:54.10 Plunge.jog 1597 @11:56:54.16 Air.in :homing @11:56:54.28 Toroid.in :homing @11:56:54.32 PinPort.in :homing @11:56:54.43 Lysis1.in :homing @11:56:54.49 Archive.in :homing @11:56:54.83 Lysis2.jog 1405 @11:56:54.88 Air.jog 1367 @11:56:54.98 Toroid.jog 623 @11:56:55.06 Lysis1.jog 1392 @11:56:55.14 Archive.jog 1395 @11:56:55.19 PinPort.jog -109 @11:56:55.73 PinPort.setRawPosition! 0 @11:56:55.81 PinPort.seek :home @11:56:55.88 Plunge.setRawPosition! 50 @11:56:56.02 Plunge.seek :home @11:56:56.15 PinPort.in :default @11:56:56.25 Lysis2.setRawPosition! 50 @11:56:56.40 Plunge.in :default @11:56:56.48 Lysis2.seek :home @11:56:56.64 Lysis1.setRawPosition! 50 @11:56:56.74 Air.setRawPosition! 50 @11:56:56.78 Archive.setRawPosition! 50 @11:56:56.98 Lysis1.seek :home @11:56:57.02 Air.seek :home @11:56:57.07 Archive.seek :home @11:56:57.36 Lysis2.in :default @11:56:57.52 Lysis1.in :default @11:56:57.57 Air.in :default @11:56:57.60 Archive.in :default @11:56:57.65 Toroid.setRawPosition! 0 @11:56:57.90 Toroid.seek :home @11:56:58.02 Toroid.in :default @11:56:58.18 Toroid.seek :clear @11:56:59.94 Carousel.in :default @11:57:00.12 Carousel.home.seek 44 @11:57:00.47 Selecting Cartridge 42 @11:57:00.84 Carousel.seek 42 @11:57:01.61 Lysis1.in :fast @11:57:01.69 Lysis1.seek :locked @11:57:04.35 Plunge.seek :locked @11:57:04.53 Air.in :fast @11:57:04.62 Air.seek :locked @11:57:05.65 Carousel.reconfigure :locked @11:57:05.83 Powering cartridge bus @11:57:08.53 Gate.setIR 1750,5000,0,6 @11:57:08.66 Gate.setIR 0,5000,0,6 @11:57:08.79 PuckHeater.configure :default #ambient 27.6C @11:57:08.89 Gate.power :heat,:ON,limit to 5000mA @11:57:10.03 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @11:57:10.20 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:57:10.25 PhytoSampler with {source: :sampler} @11:57:10.28 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 25 ml",Subject: "Sampling microSPR_phyto cartridge in slot 42" @11:57:10.73 Preparing to filter cartridge 42 @11:57:11.03 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:57:15.98 upsync @11:58:39.58 Session Begun @11:58:48.26 -> Syringe.psi -0.683408273789269 @11:59:34.66 Intake Head Pressure tare is 4% of full scale at 26C (drifted 3%) @11:59:34.83 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @11:59:34.93 PhaseSeparator.in :filling @11:59:35.02 PhaseSeparator.hold @11:59:35.15 Gate.power :sampler,:ON @11:59:42.04 Head pressure reached 1.99psi in 8 seconds @11:59:42.07 Air.in :default @11:59:42.30 Priming sample loop w/150ml, bypass w/50ml @11:59:42.33 SamplePump.setPosition! 0ml @11:59:42.39 Air.seek :sealed @11:59:42.54 Bypass.open @11:59:44.14 Intake.open @11:59:44.96 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:59:47.58 Vacuum tare is 51% of full scale at 26C (drifted 0%) @11:59:47.77 Abs.in :default @11:59:47.90 Abs.hold @11:59:48.04 PhaseSeparator.in :full @11:59:48.13 PhaseSeparator.hold @11:59:48.42 Waiting for sampler to finish priming... @11:59:48.45 delayUntil "12:01:05.17" @12:00:44.35 -> Syringe.psi -0.593548362356785 @12:01:05.25 SamplePump.in :start @12:01:05.42 SamplePump.seek 50ml,3:50 @12:01:05.94 SamplePump.configure :prime @12:02:06.88 Abs.stop @12:02:06.98 PhaseSeparator.stop @12:02:07.09 SamplePump.setPosition! 0ml @12:02:07.18 Exhaust.open @12:02:08.60 Bypass.close @12:02:10.27 Abs.in :default @12:02:10.36 Abs.hold @12:02:10.50 PhaseSeparator.hold @12:02:10.92 SamplePump.in :start @12:02:11.04 SamplePump.seek 150ml,10:30 @12:02:11.61 SamplePump.configure :prime @12:05:14.92 Abs.stop @12:05:15.06 PhaseSeparator.stop @12:05:15.18 Bypass.open @12:05:15.98 Intake.close @12:05:16.82 Toroid.in :hold @12:05:16.94 Toroid.seek :crack @12:05:18.52 Toroid.seek :cartridge @12:05:20.16 Toroid.in :default @12:05:20.36 Toroid.seek :clear @12:05:22.46 Pressurizing cartridge to 26psi above 14.6psia @12:05:22.49 SamplePump.setPosition! 0ml @12:05:22.61 Intake.open @12:05:23.36 Bypass.close @12:05:24.07 Exhaust.close @12:05:24.90 Abs.in :default @12:05:25.00 Abs.hold @12:05:25.11 PhaseSeparator.hold @12:05:25.41 SamplePump.in :charge @12:05:25.52 SamplePump.seek 2.3ml,42 seconds @12:05:38.51 Abs.stop @12:05:38.63 PhaseSeparator.stop @12:05:38.77 Intake.close @12:05:39.58 Pressurized cartridge with 0.756ml to 26.1psi @12:05:39.60 Isolated @34.3psia (19.7psi) @12:05:39.66 Verifying cartridge holds at least 0.65..0.85 of 34.3psia @12:05:39.72 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:05:45.36 Cartridge pressure fell 12% to 30.1psia @12:05:45.51 Sampling 25.0ml @12:05:45.53 SamplePump.setPosition! 0ml @12:05:45.65 Exhaust.open @12:05:46.34 Intake.open @12:05:47.27 Abs.in :default @12:05:47.38 Abs.hold @12:05:47.50 PhaseSeparator.hold @12:05:47.91 SamplePump.in :start @12:05:48.03 SamplePump.seek 25ml,2:35 @12:05:48.60 SamplePump.configure :sample @12:05:51.00 Cartridge 42 filtering 2/25ml at 0.64ml/s with 26.7psi vacuum @12:06:30.91 Abs.stop @12:06:31.01 PhaseSeparator.stop @12:06:31.21 Intake.close @12:06:31.33 Exhaust.close @12:06:32.23 Toroid.in :hold @12:06:32.35 Toroid.seek :bypass @12:06:34.03 Toroid.in :default @12:06:34.14 Toroid.seek :clear @12:06:35.80 Sampled 25.0ml @12:06:36.32 GoodNews.email "After filtering 25.0ml",Subject: "Processing microSPR_phyto cartridge in slot 42" @12:06:38.02 SPR.command "read_sequences" @12:06:38.27 SPR.command "stats_on" @12:06:38.51 SPR.command "start 0 1" @12:06:38.98 Gate.power :sampler,:OFF @12:06:39.21 SPR.start /var/log/esp/spr24aug0712h.spr.bz2 @12:06:41.16 Handoff.select :waste @12:06:41.37 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:06:41.96 upsync @12:06:48.01 Syringe tare is 51% of full scale at 26C (drifted 0%) @12:06:48.09 PinPort.in :locking @12:06:48.19 PinPort.seek :locked @12:06:48.76 Archive.in :fast @12:06:48.91 Lysis2.in :fast @12:06:48.97 Lysis1.seek 1350uL @12:06:49.04 Archive.seek :full @12:06:49.30 Lysis2.seek 950uL @12:06:54.17 Plunge.seek :reagent @12:06:55.14 Evacuating puck with filter bubble point of 24psi @12:06:55.71 PinPort.seek :locked @12:06:55.93 Handoff.select :waste @12:06:56.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:07:02.61 Air syringe pumping up to 1 strokes to reach 8psi @12:07:02.63 Air stroke #1 of 1 @12:07:02.82 Air.in :pressurize @12:07:02.93 Air.seek :pressurized @12:07:08.59 1.9psi after pressurize @12:07:08.65 Air.in :compress @12:07:08.80 Air.seek :evac @12:07:11.88 2.3psi after compress @12:07:22.50 1.2psi after compress (stable) @12:07:22.52 Air.in :default @12:07:22.73 Air.hold @12:07:23.07 at 0.7psi Opened to waste @12:07:23.09 Handoff.select :blocked @12:07:23.30 Archive syringe pumping up to 8 strokes to reach 24psi @12:07:23.32 Archive stroke #1 of 8 @12:07:23.48 Archive.in :pressurize @12:07:24.03 Archive.seek :pressurized @12:07:36.67 2.0psi after pressurize @12:07:42.30 1.5psi after pressurize (stable) @12:07:42.32 Archive.in :compress @12:07:42.46 Archive.seek :empty @12:07:58.27 14.3psi after compress @12:08:08.87 14.1psi after compress (stable) @12:08:08.93 Archive.in :gulp @12:08:09.04 Archive.seek :topVent @12:08:15.40 14.9psi after gulp @12:08:20.99 14.4psi after gulp (stable) @12:08:21.01 Archive stroke #2 of 8 @12:08:21.18 Archive.in :pressurize @12:08:21.29 Archive.seek :pressurized @12:08:33.77 14.8psi after pressurize @12:08:39.37 14.1psi after pressurize (stable) @12:08:39.41 Archive.in :compress @12:08:39.54 Archive.seek :empty @12:08:52.63 Reached 24.1 of 24.0psi after pumping 3402uL (1.97 strokes) @12:08:52.66 Archive.in :default @12:08:52.83 Archive.hold @12:08:52.95 Handoff.select :waste @12:08:53.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:09:06.26 delay 1:00 @12:10:06.31 Handoff.select :blocked @12:10:06.49 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:10:29.09 Handoff.select :waste @12:10:29.29 0.5psi at evacEnd @12:10:29.31 Handoff.select :blocked @12:10:29.54 Archive.in :fast @12:10:29.69 Archive.seek :topVent @12:10:36.73 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @12:10:43.32 Stable @0.3psi after Handoff blocked @12:10:43.39 Archive syringe pumping up to 8 strokes to reach 24psi @12:10:43.41 Archive stroke #1 of 8 @12:10:43.56 Archive.in :pressurize @12:10:43.67 Archive.seek :pressurized @12:10:56.20 1.0psi after pressurize @12:10:56.24 Archive.in :compress @12:10:56.36 Archive.seek :empty @12:11:12.17 14.9psi after compress @12:11:22.77 13.9psi after compress (stable) @12:11:22.80 Archive.in :gulp @12:11:22.93 Archive.seek :topVent @12:11:29.78 14.7psi after gulp @12:11:35.39 13.9psi after gulp (stable) @12:11:35.41 Archive stroke #2 of 8 @12:11:35.57 Archive.in :pressurize @12:11:35.67 Archive.seek :pressurized @12:11:48.18 14.4psi after pressurize @12:11:48.23 Archive.in :compress @12:11:48.36 Archive.seek :empty @12:12:01.90 Reached 24.3 of 24.3psi after pumping 3412uL (1.97 strokes) @12:12:01.93 Archive.in :default @12:12:02.07 Archive.hold @12:12:02.20 Handoff.select :waste @12:12:02.44 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:12:10.05 delay 1:00 @12:13:10.16 Handoff.select :blocked @12:13:10.38 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:13:22.82 -> Syringe.psi 0.327515729826359 @12:13:32.98 Handoff.select :waste @12:13:33.21 0.4psi at evacEnd @12:13:33.24 Air.seek :fullVent @12:13:34.64 Archive.in :fast @12:13:34.78 Archive.seek :topVent @12:13:41.81 Seawater evacuated, Pressure above/below filter released. @12:13:41.96 Lysis1.in :slow @12:13:42.06 Lysis1.seek 1050uL @12:14:11.73 delay 5 seconds @12:14:16.75 Handoff.select :blocked @12:14:17.00 Lysis1.seek 450uL @12:15:16.20 delay 3 seconds @12:15:19.29 Air.in mix with 1.57127 parts Lysis1 @12:15:20.03 Air.seek :evac @12:15:20.13 Lysis1.seek 300uL @12:15:24.43 -> Syringe.psi 5.47317802659273 @12:15:35.14 Lysis1.seek :empty @12:15:37.06 -> Syringe.psi 9.37498997037238 @12:15:43.94 -> Syringe.psi 12.7943960738302 @12:15:50.02 -> Syringe.psi 16.7955268670878 @12:16:00.65 -> Syringe.psi 28.117875707583 @12:16:04.92 Lysis buffer delivered @34.8psi @12:16:04.94 Polling Can every 2:00 @12:16:05.53 PuckHeater.configure :default #ambient 26.1C @12:16:05.64 PuckHeater.seek 79.6C @12:16:06.59 -> Syringe.psi 34.0060646409231 @12:16:40.92 -> Syringe.psi 36.2762097718495 @12:16:54.80 PuckHeater.hold 2:00,79.6C @12:17:21.22 -> Syringe.psi 39.6104854328975 @12:18:54.64 Polling Can every 2:00 @12:18:55.25 PuckHeater.configure :default #ambient 26.1C @12:18:55.35 PuckHeater.hold 30 seconds,78.5C @12:19:25.51 Polling Can every 2:00 @12:19:26.07 PuckHeater.hold 30 seconds,78C @12:19:56.24 Polling Can every 2:00 @12:19:56.80 PuckHeater.configure :default #ambient 26.1C @12:19:56.92 PuckHeater.hold 10:00,77.5C @12:20:55.32 -> Syringe.psi 41.7529349002093 @12:25:50.37 -> Syringe.psi 41.9610315372108 @12:29:09.09 -> Syringe.psi 41.9941378203702 @12:29:55.44 Lysis complete. Syringe pressure @41.9psi @12:29:55.48 Archive syringe pumping up to 5 strokes to reach 40psi @12:29:55.53 Archive stroke #1 of 5 @12:29:55.67 Archive.in :pressurize @12:29:55.78 Archive.seek :pressurized @12:29:56.05 Reached 42.5 of 40.0psi after pumping 0uL (0.00 strokes) @12:29:56.08 Archive.in :default @12:29:56.20 Archive.hold @12:29:56.33 delay 2 seconds @12:29:58.36 Archive.in :fast @12:29:58.46 Archive.seek :full @12:29:58.77 Lysis1.seek 138uL @12:30:01.86 -> Syringe.psi 37.0140069394005 @12:30:12.41 -> Syringe.psi @12:30:12.56 delay 1 second 23.5350202245254 @12:30:13.60 Archive.seek :full @12:30:13.71 Lysis1.seek :empty @12:30:27.45 -> Syringe.psi 40.9158188831802 @12:30:28.01 Handoff.select :blocked @12:30:28.17 Initial air pressure @41.9psi @12:30:28.29 Lysis1.in :extract @12:30:28.40 Lysis1.seek 600uL,nil @12:30:30.95 Archive syringe pumping up to 6 strokes to reach 25psi @12:30:30.96 Archive stroke #1 of 6 @12:30:31.12 Archive.in :pressurize @12:30:31.24 Archive.seek :pressurized @12:30:43.74 11.4psi after pressurize @12:30:43.89 -> Syringe.psi 10.7891012081786 @12:30:49.34 10.9psi after pressurize (stable) @12:30:49.37 Archive.in :compress @12:30:49.51 Archive.seek :empty @12:30:58.83 Archive.in :default @12:30:59.00 Archive.hold @12:30:59.31 Initial 600uL lysate @18.4psi @12:30:59.33 Handoff.select :blocked @12:30:59.51 Initial air pressure @18.9psi @12:30:59.61 Lysis1.seek 780uL,nil @12:31:01.61 Archive syringe pumping up to 6 strokes to reach 17psi @12:31:01.63 Archive stroke #1 of 6 @12:31:01.81 Archive.hold @12:31:02.20 Recovered 780uL lysate @15.6psi @12:31:02.24 Air.in :halfSpeed @12:31:02.37 Air.seek :fullVent @12:31:04.42 Puck Top Vented, bottom @17.0psi @12:31:04.44 Handoff.select :waste @12:31:04.64 delay 5 seconds @12:31:08.44 -> Syringe.psi 1.10787811858228 @12:31:09.72 Handoff open to waste, bottom @1.2psi @12:31:09.76 Air.in :default @12:31:09.88 Air.seek :evac @12:31:11.25 Lysis2.in :DAmix @12:31:11.46 Lysis1.in :DAmix @12:31:12.20 Lysis2.seek 700uL @12:31:12.27 Lysis1.seek 530uL @12:31:12.43 -> Syringe.psi 1.50042404747163 @12:31:15.56 -> Syringe.psi 1.94499413561138 @12:31:21.07 -> Syringe.psi 1.80311006492849 @12:31:24.89 -> Syringe.psi 1.80783953395124 @12:31:28.09 -> Syringe.psi 2.61184926782099 @12:31:31.31 -> Syringe.psi 1.94972360463413 @12:31:34.49 -> Syringe.psi 1.99701829486176 @12:31:37.69 -> Syringe.psi 2.14836130359021 @12:31:40.90 -> Syringe.psi 1.95445307365691 @12:31:44.09 -> Syringe.psi 1.89769944538375 @12:31:47.07 delay 5 seconds @12:31:47.70 -> Syringe.psi 0.455211393440976 @12:31:50.67 -> Syringe.psi 0.554530242918986 @12:31:52.11 Handoff.select SPR @12:31:52.30 SPR.command "start 2 14" @12:31:53.51 SPR stabilized @12:31:53.73 delay 10 seconds @12:31:54.42 -> Syringe.psi 0.54507130487346 @12:31:58.82 -> Syringe.psi 0.767356348943338 @12:32:02.65 -> Syringe.psi 0.743709003829537 @12:32:03.75 SPR.command "start 2 0" @12:32:06.89 Waiting up to 11:00 for SPR to enter state 1 @12:32:11.30 -> Syringe.psi 0.895052012557954 @12:32:20.26 -> Syringe.psi 0.951805640831111 @12:32:26.32 -> Syringe.psi 0.885593074512428 @12:32:42.95 -> Syringe.psi 1.01801820714979 @12:32:55.26 -> Syringe.psi 0.965994047899386 @12:33:08.87 -> Syringe.psi 0.975452985944912 @12:33:26.96 -> Syringe.psi 0.899781481580703 @12:33:38.98 -> Syringe.psi 1.12679599467333 @12:33:50.73 -> Syringe.psi 0.923428826694533 @12:34:04.55 SPR.command "hold_on" @12:34:06.42 -> Syringe.psi 0.606554402169394 @12:34:06.65 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:34:07.52 Lysis2.seek 400uL @12:34:07.65 Lysis1.seek 230uL @12:34:10.46 -> Syringe.psi 2.5456367015023 @12:34:14.62 -> Syringe.psi 2.74900386948113 @12:34:17.82 -> Syringe.psi 2.93345316136887 @12:34:20.72 -> Syringe.psi 2.93345316136887 @12:34:24.54 -> Syringe.psi 2.96182997550545 @12:34:28.38 -> Syringe.psi 2.85778165700467 @12:34:35.43 -> Syringe.psi 2.78683962166323 @12:34:43.12 -> Syringe.psi 2.69697971023072 @12:34:49.14 delay 15 seconds @12:34:50.68 -> Syringe.psi 1.47677670235782 @12:35:03.98 -> Syringe.psi 0.729520596761233 @12:35:04.20 SPR.command "hold_off" @12:35:07.54 Waiting up to 1:00:00 for SPR to enter state 7 @12:35:09.10 -> Syringe.psi 0.852486791353073 @12:35:12.52 -> Syringe.psi 0.658578561419773 @12:35:16.71 -> Syringe.psi 0.805192101125442 @12:35:20.21 -> Syringe.psi 0.843027853307547 @12:35:23.10 -> Syringe.psi 0.66330803044255 @12:35:30.45 -> Syringe.psi 0.828839446239272 @12:35:35.89 -> Syringe.psi 0.634931216305972 @12:35:40.37 -> Syringe.psi 0.795733163079916 @12:35:43.88 -> Syringe.psi 0.696414313601878 @12:51:22.16 SPRsummary:-1.08RIU,4.8e+08RIU,2.13e+08RIU,1.44e+09RIU,none,none,none after 2834s @13:05:23.32 -> Syringe.psi 0.318056791780833 @13:05:39.07 SPR analysis completed @13:05:48.04 Ended log spr24aug0712h.spr.bz2 @13:05:48.07 SPR.command "stats_off" @13:05:49.64 Handoff.select :close @13:05:49.83 Gate.power :heat,:OFF @13:05:49.86 Gate.power :cartridge,:OFF @13:05:50.00 PinPort.in :unlocking @13:05:50.08 PinPort.seek :clear @13:05:50.60 Carousel.in :default @13:05:50.80 Carousel.home.seek 42 @13:05:51.17 Plunge.seek :locked @13:05:52.11 Archive.in :unlocking @13:05:52.29 Archive.seek :clear @13:05:52.43 Air.in :unlocking @13:05:52.58 Air.seek :clear @13:05:52.77 Lysis1.in :unlocking @13:05:52.93 Lysis1.seek :clear @13:05:53.08 Lysis2.in :unlocking @13:05:53.19 Lysis2.seek :clear @13:05:57.14 Gate.power :analytic1,:OFF @13:05:57.16 Gate.power :motor,:OFF @13:05:57.22 Gate.power :core,:OFF @13:05:57.27 Gate.power :main,:OFF @13:05:57.31 Safely stopped and ready to power off @13:06:03.00 -> exit @13:06:03.03 Session Ended @13:12:27.74 Session Ended @16:45:17.15 Session Begun @16:45:51.48 -> PhytoSampler.xray @16:49:36.72 -> PhytoSampler.goalVolume=1000 1000 @17:04:31.57 -> deep; fire lysis # @17:04:32.42 Gate.power :sampler,:OFF,limit to 3500mA @17:04:32.49 Gate.power :skimmer,:OFF,limit to 3500mA @17:04:32.53 Gate.power :main,:ON,limit to 5000mA @17:04:32.57 Gate.power :analytic1,:ON,limit to 4000mA @17:04:32.69 Powering core bus @17:04:35.02 SPR Module 1, FW Rev. B, FPGA Rev. C @17:04:35.49 Plunge.configure :default @17:04:35.61 SamplePump.configure :default @17:04:35.71 Toroid.configure :default @17:04:35.82 Carousel.configure :default @17:04:35.94 Abs.configure :default @17:04:36.03 PhaseSeparator.configure :full @17:04:36.15 Archive.configure :default @17:04:36.26 Air.configure :default @17:04:36.34 Lysis1.configure :default @17:04:36.43 Lysis2.configure :default @17:04:36.53 PinPort.configure :default @17:04:36.63 All elf controllers running firmware version 4.92 @17:04:36.66 Gate.power :motor,:ON,limit to 5000mA @17:04:37.04 Plunge.home.jog 0 @17:04:37.78 PinPort.home.jog 0 @17:04:37.82 Lysis1.home.jog 0 @17:04:37.87 Carousel.home.jog 0 @17:04:37.93 Air.home.jog 0 @17:04:37.96 Archive.home.jog 0 @17:04:38.01 Lysis2.home.jog 0 @17:04:38.05 Toroid.home.jog 0 @17:04:39.15 Plunge.in :homing @17:04:39.31 Plunge.jog 1601 @17:04:39.57 PinPort.in :homing @17:04:39.61 Lysis2.in :homing @17:04:39.66 Air.in :homing @17:04:39.69 Toroid.in :homing @17:04:39.73 Lysis1.in :homing @17:04:39.78 Archive.in :homing @17:04:40.09 PinPort.jog -111 @17:04:40.14 Lysis2.jog 1402 @17:04:40.20 Lysis1.jog 1393 @17:04:40.26 Air.jog 1367 @17:04:40.30 Toroid.jog 620 @17:04:40.35 Archive.jog 1397 @17:04:40.91 PinPort.setRawPosition! 0 @17:04:40.93 Plunge.setRawPosition! 50 @17:04:41.08 PinPort.seek :home @17:04:41.11 Plunge.seek :home @17:04:41.36 PinPort.in :default @17:04:41.50 Plunge.in :default @17:04:41.75 Lysis2.setRawPosition! 50 @17:04:41.81 Lysis1.setRawPosition! 50 @17:04:41.88 Air.setRawPosition! 50 @17:04:41.95 Lysis2.seek :home @17:04:42.08 Lysis1.seek :home @17:04:42.25 Air.seek :home @17:04:42.92 Lysis2.in :default @17:04:43.04 Archive.setRawPosition! 50 @17:04:43.19 Archive.seek :home @17:04:43.36 Air.in :default @17:04:43.38 Toroid.setRawPosition! 0 @17:04:43.42 Lysis1.in :default @17:04:43.65 Toroid.seek :home @17:04:43.80 Archive.in :default @17:04:43.84 Toroid.in :default @17:04:44.22 Toroid.seek :clear @17:04:45.98 Carousel.in :default @17:04:46.14 Carousel.home.seek 42 @17:04:46.46 Selecting Cartridge 40 @17:04:46.83 Carousel.seek 40 @17:04:47.60 Lysis1.in :fast @17:04:47.68 Lysis1.seek :locked @17:04:50.34 Plunge.seek :locked @17:04:50.52 Air.in :fast @17:04:50.60 Air.seek :locked @17:04:51.61 Carousel.reconfigure :locked @17:04:51.80 Powering cartridge bus @17:04:54.47 Gate.setIR 1750,5000,0,6 @17:04:54.60 Gate.setIR 0,5000,0,6 @17:04:54.73 PuckHeater.configure :default #ambient 26.1C @17:04:54.82 Gate.power :heat,:ON,limit to 5000mA @17:04:55.53 Mounted lysis Cartridge board#Q05041 @17:04:55.59 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:04:55.64 PhytoSampler with {source: :sampler} @17:04:55.66 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling microSPR_phyto cartridge in slot 40" @17:04:56.06 Preparing to filter cartridge 40 @17:04:56.37 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @17:05:01.52 upsync @17:05:16.50 Intake Head Pressure tare is 4% of full scale at 23C (drifted 3%) @17:05:16.53 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @17:05:16.59 PhaseSeparator.in :filling @17:05:16.66 PhaseSeparator.hold @17:05:16.81 Gate.power :sampler,:ON @17:05:23.68 Head pressure reached 1.88psi in 8 seconds @17:05:23.71 Air.in :default @17:05:23.91 Priming sample loop w/150ml, bypass w/50ml @17:05:23.93 Air.seek :sealed @17:05:23.98 SamplePump.setPosition! 0ml @17:05:24.12 Bypass.open @17:05:25.60 Intake.open @17:05:26.38 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:05:29.00 Vacuum tare is 51% of full scale at 23C (drifted 0%) @17:05:29.20 Abs.in :default @17:05:29.29 Abs.hold @17:05:29.39 PhaseSeparator.in :full @17:05:29.48 PhaseSeparator.hold @17:05:29.75 Waiting for sampler to finish priming... @17:05:29.76 delayUntil "17:06:46.83" @17:06:10.63 -> exit @17:06:11.18 Session Ended @17:06:46.90 SamplePump.in :start @17:06:46.96 SamplePump.seek 50ml,3:50 @17:06:47.59 SamplePump.configure :prime @17:07:48.37 Abs.stop @17:07:48.42 PhaseSeparator.stop @17:07:48.47 SamplePump.setPosition! 0ml @17:07:48.52 Exhaust.open @17:07:49.85 Bypass.close @17:07:51.40 Abs.in :default @17:07:51.46 Abs.hold @17:07:51.55 PhaseSeparator.hold @17:07:51.88 SamplePump.in :start @17:07:51.94 SamplePump.seek 150ml,10:30 @17:07:52.57 SamplePump.configure :prime @17:10:55.77 Abs.stop @17:10:55.81 PhaseSeparator.stop @17:10:55.96 Bypass.open @17:10:56.65 Intake.close @17:10:57.45 Toroid.in :hold @17:10:57.50 Toroid.seek :crack @17:10:59.04 Toroid.seek :cartridge @17:11:00.16 Toroid.in :default @17:11:00.21 Toroid.seek :clear @17:11:02.27 Pressurizing cartridge to 26psi above 14.7psia @17:11:02.29 SamplePump.setPosition! 0ml @17:11:02.34 Intake.open @17:11:02.99 Bypass.close @17:11:03.65 Exhaust.close @17:11:04.38 Abs.in :default @17:11:04.43 Abs.hold @17:11:04.51 PhaseSeparator.hold @17:11:04.78 SamplePump.in :charge @17:11:04.84 SamplePump.seek 2.3ml,42 seconds @17:11:17.70 Abs.stop @17:11:17.76 PhaseSeparator.stop @17:11:17.82 Intake.close @17:11:18.53 Pressurized cartridge with 0.743ml to 27.4psi @17:11:18.55 Isolated @35.2psia (20.6psi) @17:11:18.56 Verifying cartridge holds at least 0.65..0.85 of 35.2psia @17:11:18.59 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:11:23.18 Cartridge pressure fell 3% to 34.0psia @17:11:23.29 Sampling 1000.0ml @17:11:23.31 SamplePump.setPosition! 0ml @17:11:23.36 Exhaust.open @17:11:23.98 Intake.open @17:11:24.86 Abs.in :default @17:11:24.91 Abs.hold @17:11:24.99 PhaseSeparator.hold @17:11:25.34 SamplePump.in :start @17:11:25.40 SamplePump.seek 1000ml,1:23:50 @17:11:26.03 SamplePump.configure :sample @17:11:28.44 Cartridge 40 filtering 2/1000ml at 0.61ml/s with 21.7psi vacuum @17:12:28.49 Cartridge 40 filtering 33/1000ml at 0.52ml/s with 30.1psi vacuum @17:13:28.49 Cartridge 40 filtering 60/1000ml at 0.46ml/s with 22.0psi vacuum @17:14:28.49 Cartridge 40 filtering 85/1000ml at 0.42ml/s with 20.8psi vacuum @17:15:28.50 Cartridge 40 filtering 108/1000ml at 0.37ml/s with 21.7psi vacuum @17:16:28.50 Cartridge 40 filtering 127/1000ml at 0.32ml/s with 19.7psi vacuum @17:17:28.49 Cartridge 40 filtering 143/1000ml at 0.27ml/s with 23.3psi vacuum @17:18:28.50 Cartridge 40 filtering 156/1000ml at 0.22ml/s with 20.3psi vacuum @17:19:28.50 Cartridge 40 filtering 167/1000ml at 0.18ml/s with 14.5psi vacuum 5.3m deep (22.5psia) @17:19:28.55 SamplePump.stop @17:19:28.62 Intake.close @17:19:28.74 Exhaust.close @17:19:28.97 Toroid.in :hold @17:19:29.03 Toroid.seek :bypass @17:19:30.69 Toroid.in :default @17:19:30.76 Toroid.seek :clear @17:19:32.38 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @17:19:32.40 Abs.stop @17:19:32.44 PhaseSeparator.stop @17:19:32.69 Sampled 167.3ml @17:19:33.17 GoodNews.email "After filtering 167.3ml",Subject: "Processing microSPR_phyto cartridge in slot 40" @17:19:34.81 SPR.command "read_sequences" @17:19:35.34 SPR.command "stats_on" @17:19:35.53 SPR.command "start 0 1" @17:19:35.85 Gate.power :sampler,:OFF @17:19:36.21 SPR.start /var/log/esp/spr24aug0717h.spr.bz2 @17:19:38.73 Handoff.select :waste @17:19:38.89 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:19:39.09 upsync @17:19:45.54 Syringe tare is 51% of full scale at 24C (drifted 0%) @17:19:45.56 PinPort.in :locking @17:19:45.61 PinPort.seek :locked @17:19:46.12 Archive.in :fast @17:19:46.24 Lysis2.in :fast @17:19:46.33 Lysis1.seek 1350uL @17:19:46.36 Archive.seek :full @17:19:46.51 Lysis2.seek 950uL @17:19:51.29 Plunge.seek :reagent @17:19:52.20 Evacuating puck with filter bubble point of 24psi @17:19:52.22 PinPort.seek :locked @17:19:52.32 Handoff.select :waste @17:19:52.35 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:19:59.50 Air syringe pumping up to 1 strokes to reach 8psi @17:19:59.52 Air stroke #1 of 1 @17:19:59.66 Air.in :pressurize @17:19:59.72 Air.seek :pressurized @17:20:05.34 0.6psi after pressurize @17:20:05.37 Air.in :compress @17:20:05.42 Air.seek :evac @17:20:08.44 1.8psi after compress @17:20:19.03 0.1psi after compress (stable) @17:20:19.05 Air.in :default @17:20:19.11 Air.hold @17:20:19.36 at 0.1psi Opened to waste @17:20:19.39 Handoff.select :blocked @17:20:19.52 Archive syringe pumping up to 8 strokes to reach 24psi @17:20:19.54 Archive stroke #1 of 8 @17:20:19.62 Archive.in :pressurize @17:20:19.67 Archive.seek :pressurized @17:20:32.11 7.3psi after pressurize @17:20:46.77 9.3psi after pressurize (stable) @17:20:46.81 Archive.in :compress @17:20:46.86 Archive.seek :empty @17:21:01.54 Reached 24.3 of 24.0psi after pumping 1707uL (0.99 strokes) @17:21:01.56 Archive.in :default @17:21:01.61 Archive.hold @17:21:01.69 Handoff.select :waste @17:21:01.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:21:19.97 delay 1:00 @17:22:19.99 Handoff.select :blocked @17:22:20.13 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:22:42.75 Handoff.select :waste @17:22:42.88 0.9psi at evacEnd @17:22:42.89 Handoff.select :blocked @17:22:43.02 Archive.in :fast @17:22:43.07 Archive.seek :topVent @17:22:50.21 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @17:22:56.81 Stable @0.7psi after Handoff blocked @17:22:56.84 Archive syringe pumping up to 8 strokes to reach 25psi @17:22:56.85 Archive stroke #1 of 8 @17:22:56.94 Archive.in :pressurize @17:22:57.00 Archive.seek :pressurized @17:23:09.44 1.4psi after pressurize @17:23:09.47 Archive.in :compress @17:23:09.52 Archive.seek :empty @17:23:25.29 17.0psi after compress @17:23:35.88 16.0psi after compress (stable) @17:23:35.91 Archive.in :gulp @17:23:35.96 Archive.seek :topVent @17:23:42.31 17.3psi after gulp @17:23:47.99 16.7psi after gulp (stable) @17:23:48.01 Archive stroke #2 of 8 @17:23:48.10 Archive.in :pressurize @17:23:48.15 Archive.seek :pressurized @17:24:00.60 17.1psi after pressurize @17:24:00.66 Archive.in :compress @17:24:00.78 Archive.seek :empty @17:24:11.87 Reached 24.9 of 24.7psi after pumping 3360uL (1.94 strokes) @17:24:11.89 Archive.in :default @17:24:11.94 Archive.hold @17:24:12.05 Handoff.select :waste @17:24:12.21 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:24:23.81 delay 1:00 @17:25:23.83 Handoff.select :blocked @17:25:24.00 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:25:46.64 Handoff.select :waste @17:25:46.81 0.5psi at evacEnd @17:25:46.83 Air.seek :fullVent @17:25:48.15 Archive.in :fast @17:25:48.21 Archive.seek :topVent @17:25:55.02 Seawater evacuated, Pressure above/below filter released. @17:25:55.15 Lysis1.in :slow @17:25:55.22 Lysis1.seek 1050uL @17:26:24.89 delay 5 seconds @17:26:29.91 Handoff.select :blocked @17:26:30.15 Lysis1.seek 450uL @17:27:29.37 delay 3 seconds @17:27:32.47 Air.in mix with 1.57127 parts Lysis1 @17:27:33.28 Air.seek :evac @17:27:33.33 Lysis1.seek 300uL @17:27:48.30 Lysis1.seek :empty @17:28:18.03 Lysis buffer delivered @18.6psi @17:28:18.05 Polling Can every 2:00 @17:28:18.60 PuckHeater.configure :default #ambient 24.2C @17:28:18.65 PuckHeater.seek 79.6C @17:29:16.43 PuckHeater.hold 2:00,79.6C @17:31:16.34 Polling Can every 2:00 @17:31:16.89 PuckHeater.configure :default #ambient 24.3C @17:31:16.95 PuckHeater.hold 30 seconds,78.6C @17:31:47.12 Polling Can every 2:00 @17:31:47.67 PuckHeater.hold 30 seconds,78.1C @17:32:17.81 Polling Can every 2:00 @17:32:18.36 PuckHeater.hold 10:00,77.6C @17:42:17.54 Lysis complete. Syringe pressure @24.8psi @17:42:17.56 Archive syringe pumping up to 5 strokes to reach 40psi @17:42:17.58 Archive stroke #1 of 5 @17:42:17.66 Archive.in :pressurize @17:42:17.72 Archive.seek :pressurized @17:42:30.20 24.9psi after pressurize @17:42:35.82 24.1psi after pressurize (stable) @17:42:35.86 Archive.in :compress @17:42:35.92 Archive.seek :empty @17:42:44.42 Reached 40.9 of 40.0psi after pumping 1564uL (0.90 strokes) @17:42:44.44 Archive.in :default @17:42:44.50 Archive.hold @17:42:44.59 delay 2 seconds @17:42:46.61 Archive.in :fast @17:42:46.66 Archive.seek :full @17:42:53.36 Lysis1.seek 138uL @17:43:07.06 delay 1 second @17:43:08.10 Archive.seek :full @17:43:08.16 Lysis1.seek :empty @17:43:21.93 Handoff.select :blocked @17:43:22.07 Initial air pressure @40.4psi @17:43:22.18 Lysis1.in :extract @17:43:22.28 Lysis1.seek 600uL,nil @17:43:25.38 Archive syringe pumping up to 6 strokes to reach 25psi @17:43:25.39 Archive stroke #1 of 6 @17:43:25.48 Archive.in :pressurize @17:43:25.54 Archive.seek :pressurized @17:43:37.97 12.3psi after pressurize @17:43:43.60 11.8psi after pressurize (stable) @17:43:43.65 Archive.in :compress @17:43:43.72 Archive.seek :empty @17:43:49.53 Archive.in :default @17:43:49.60 Archive.hold @17:43:49.81 Initial 600uL lysate @17.6psi @17:43:49.83 Handoff.select :blocked @17:43:49.92 Initial air pressure @16.8psi @17:43:50.02 Lysis1.seek 780uL,nil @17:43:51.25 Archive syringe pumping up to 6 strokes to reach 17psi @17:43:51.27 Archive stroke #1 of 6 @17:43:51.41 Archive.in :compress @17:43:51.51 Archive.seek :empty @17:43:52.62 Archive.in :default @17:43:52.72 Archive.hold @17:43:53.00 Recovered 780uL lysate @15.6psi @17:43:53.02 Air.in :halfSpeed @17:43:53.07 Air.seek :fullVent @17:43:55.08 Puck Top Vented, bottom @16.7psi @17:43:55.09 Handoff.select :waste @17:43:55.24 delay 5 seconds @17:44:00.34 Handoff open to waste, bottom @0.5psi @17:44:00.36 Air.in :default @17:44:00.41 Air.seek :evac @17:44:01.73 Lysis2.in :DAmix @17:44:01.88 Lysis1.in :DAmix @17:44:02.64 Lysis2.seek 700uL @17:44:02.70 Lysis1.seek 530uL @17:44:37.37 delay 5 seconds @17:44:42.39 Handoff.select SPR @17:44:42.54 SPR.command "start 2 14" @17:44:43.95 SPR stabilized @17:44:44.12 delay 10 seconds @17:44:54.14 SPR.command "start 2 0" @17:44:57.33 Waiting up to 11:00 for SPR to enter state 1 @17:46:54.99 SPR.command "hold_on" @17:46:56.39 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:46:57.15 Lysis2.seek 400uL @17:46:57.19 Lysis1.seek 230uL @17:47:38.68 delay 15 seconds @17:47:53.71 SPR.command "hold_off" @17:47:57.04 Waiting up to 1:00:00 for SPR to enter state 7 @18:04:12.60 SPRsummary:-0.904RIU,-121RIU,113RIU,-3.44RIU,high,22ng/L,high after 2827s @18:18:29.51 SPR analysis completed @18:18:37.59 Ended log spr24aug0717h.spr.bz2 @18:18:37.61 SPR.command "stats_off" @18:18:37.88 Handoff.select :close @18:18:38.04 Gate.power :heat,:OFF @18:18:38.06 Gate.power :cartridge,:OFF @18:18:38.14 PinPort.in :unlocking @18:18:38.19 PinPort.seek :clear @18:18:38.69 Carousel.in :default @18:18:38.83 Carousel.home.seek 40 @18:18:39.16 Plunge.seek :locked @18:18:40.07 Archive.in :unlocking @18:18:40.20 Archive.seek :clear @18:18:40.29 Air.in :unlocking @18:18:40.42 Air.seek :clear @18:18:40.54 Lysis1.in :unlocking @18:18:40.67 Lysis1.seek :clear @18:18:40.81 Lysis2.in :unlocking @18:18:40.88 Lysis2.seek :clear @18:18:45.20 Gate.power :analytic1,:OFF @18:18:45.22 Gate.power :motor,:OFF @18:18:45.24 Gate.power :core,:OFF @18:18:45.25 Gate.power :main,:OFF @18:18:45.27 Safely stopped and ready to power off @18:28:15.41 Session Begun @18:28:20.96 -> upsync @18:28:23.76 upsync @18:28:27.28 -> exit @18:28:27.31 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @23:16:19.45 Session Begun @23:16:26.97 -> ESP.ready! @23:16:27.03 Gate.power :sampler,:OFF,limit to 3500mA @23:16:27.06 Gate.power :skimmer,:OFF,limit to 3500mA @23:16:27.09 Gate.power :main,:ON,limit to 5000mA @23:16:27.12 Gate.power :analytic1,:ON,limit to 4000mA @23:16:27.23 Powering core bus @23:16:29.58 SPR Module 1, FW Rev. B, FPGA Rev. C @23:16:30.02 Plunge.configure :default @23:16:30.12 SamplePump.configure :default @23:16:30.24 Toroid.configure :default @23:16:30.35 Carousel.configure :default @23:16:30.46 Abs.configure :default @23:16:30.57 PhaseSeparator.configure :full @23:16:30.70 Archive.configure :default @23:16:30.83 Air.configure :default @23:16:30.92 Lysis1.configure :default @23:16:30.99 Lysis2.configure :default @23:16:31.11 PinPort.configure :default @23:16:31.18 All elf controllers running firmware version 4.92 @23:16:31.21 Gate.power :motor,:ON,limit to 5000mA @23:16:31.43 Plunge.home.jog 0 @23:16:32.22 Lysis2.home.jog 0 @23:16:32.29 Lysis1.home.jog 0 @23:16:32.35 Archive.home.jog 0 @23:16:32.39 Toroid.home.jog 0 @23:16:32.43 PinPort.home.jog 0 @23:16:32.48 Carousel.home.jog 0 @23:16:32.55 Air.home.jog 0 @23:16:33.52 Plunge.in :homing @23:16:33.87 Plunge.jog 1607 @23:16:33.95 Lysis2.in :homing @23:16:34.01 Air.in :homing @23:16:34.05 Toroid.in :homing @23:16:34.10 PinPort.in :homing @23:16:34.14 Lysis1.in :homing @23:16:34.19 Archive.in :homing @23:16:35.19 Lysis2.jog 1407 @23:16:35.29 Air.jog 1371 @23:16:35.34 PinPort.jog -110 @23:16:35.39 Toroid.jog 607 @23:16:35.43 Lysis1.jog 1398 @23:16:35.53 Archive.jog 1401 @23:16:36.01 PinPort.setRawPosition! 0 @23:16:36.03 Plunge.setRawPosition! 50 @23:16:36.15 PinPort.seek :home @23:16:36.21 Plunge.seek :home @23:16:36.46 PinPort.in :default @23:16:36.59 Plunge.in :default @23:16:36.74 Lysis2.setRawPosition! 50 @23:16:36.83 Lysis2.seek :home @23:16:36.97 Lysis1.setRawPosition! 50 @23:16:37.01 Air.setRawPosition! 50 @23:16:37.05 Archive.setRawPosition! 50 @23:16:37.20 Lysis1.seek :home @23:16:37.35 Archive.seek :home @23:16:37.44 Air.seek :home @23:16:37.64 Lysis2.in :default @23:16:37.67 Lysis1.in :default @23:16:37.90 Archive.in :default @23:16:37.92 Toroid.setRawPosition! 0 @23:16:37.96 Air.in :default @23:16:38.18 Toroid.seek :home @23:16:38.31 Toroid.in :default @23:16:38.45 Toroid.seek :clear @23:16:40.15 Carousel.in :default @23:16:40.35 Carousel.home.seek 40 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @-0.06psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @23:16:44.81 -> can Can@23:16:43, 23.0C, 51% humidity, 14.4psia, 14.837V, 0.513A, 15.707Ah, 7.62W @23:17:44.82 -> upsynv :upsynv @23:17:52.02 -> upsync @23:17:54.42 upsync @23:19:04.02 -> exit @23:19:04.04 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @02:06:47.43UTC08-Aug-24 Session Begun @02:06:54.06 -> ESP.ready! @02:06:55.52 Toroid.seek :clear @02:06:55.72 Carousel.in :default @02:06:55.86 Carousel.home.seek 40 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @-0.03psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @02:07:03.59 -> can Can@02:06:58, 26.5C, 49% humidity, 14.4psia, 14.838V, 0.491A, 17.078Ah, 7.29W @02:08:35.02 -> exit @02:08:35.05 Session Ended @16:48:27.99 Session Begun @16:48:31.75 -> can Can@16:48:28, 27.9C, 44% humidity, 14.5psia, 14.840V, 0.509A, 25.483Ah, 7.55W @16:49:03.90 -> exit @16:49:03.93 Session Ended @17:12:25.14 Slide::Error in core -- Storage Carousel (SC) positionErr at 40 (sensed 40 + 34 counts) @21:31:15.60UTC08-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @21:31:19.46 >BREAK< ESP3 Gateway v1.43 for core bus @21:31:19.49 >BREAK< ESP3 Gateway v1.43 for cartridge bus @21:31:23.91 Configuration for honu by nadia@mbari.org 7/16/24 /home/esp/esp2/type/auv/honu/configure.rb @21:31:25.00 Sea pressure (Abs) tare is 6% of full scale @21:31:40.38 Polling Can every 2:00 @21:31:40.45 Waiting for espclients... @21:31:40.80 Session Begun @21:32:59.47 -> ESP.ready! @21:32:59.54 Gate.power :sampler,:OFF,limit to 3500mA @21:32:59.57 Gate.power :skimmer,:OFF,limit to 3500mA @21:32:59.60 Gate.power :main,:ON,limit to 5000mA @21:32:59.63 Gate.power :analytic1,:ON,limit to 4000mA @21:32:59.83 Powering core bus @21:33:02.07 SPR Module 1, FW Rev. B, FPGA Rev. C @21:33:02.61 Plunge.configure :default @21:33:02.74 SamplePump.configure :default @21:33:02.84 Toroid.configure :default @21:33:02.94 Carousel.configure :default @21:33:03.04 Abs.configure :default @21:33:03.14 PhaseSeparator.configure :default @21:33:03.25 Archive.configure :default @21:33:03.36 Air.configure :default @21:33:03.44 Lysis1.configure :default @21:33:03.51 Lysis2.configure :default @21:33:03.62 PinPort.configure :default @21:33:03.70 All elf controllers running firmware version 4.92 @21:33:03.74 Gate.power :motor,:ON,limit to 5000mA @21:33:04.15 Plunge.home.jog 0 @21:33:04.83 Lysis2.home.jog 0 @21:33:04.89 Air.home.jog 0 @21:33:04.94 Carousel.home.jog 0 @21:33:04.97 Lysis1.home.jog 0 @21:33:05.02 Archive.home.jog 0 @21:33:05.05 PinPort.home.jog 0 @21:33:05.12 Toroid.home.jog 0 @21:33:06.39 Plunge.in :homing @21:33:06.76 Plunge.jog 156 @21:33:06.85 PinPort.in :homing @21:33:06.91 Lysis1.in :homing @21:33:06.95 Toroid.in :homing @21:33:06.99 Lysis2.in :homing @21:33:07.04 Archive.in :homing @21:33:07.12 Air.in :homing @21:33:07.38 PinPort.jog -107 @21:33:07.41 Toroid.jog 602 @21:33:07.49 Lysis1.jog 1399 @21:33:07.64 Lysis2.jog 1403 @21:33:07.73 Air.jog 1368 @21:33:07.77 Archive.jog 1397 @21:33:08.10 Plunge.setRawPosition! 50 @21:33:08.13 PinPort.setRawPosition! 0 @21:33:08.26 Plunge.seek :home @21:33:08.30 PinPort.seek :home @21:33:08.49 PinPort.in :default @21:33:08.64 Plunge.in :default @21:33:09.01 Lysis1.setRawPosition! 50 @21:33:09.10 Lysis1.seek :home @21:33:09.24 Lysis2.setRawPosition! 50 @21:33:09.28 Air.setRawPosition! 50 @21:33:09.31 Archive.setRawPosition! 50 @21:33:09.52 Air.seek :home @21:33:09.59 Lysis2.seek :home @21:33:09.65 Archive.seek :home @21:33:09.91 Lysis1.in :default @21:33:09.95 Toroid.setRawPosition! 0 @21:33:10.12 Lysis2.in :default @21:33:10.19 Air.in :default @21:33:10.25 Toroid.seek :home @21:33:10.47 Archive.in :default @21:33:10.49 Toroid.in :default @21:33:10.94 Toroid.seek :clear @21:33:12.65 Carousel.in :default @21:33:12.81 Carousel.home.seek 40 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @13psia PhaseSeparator = Intake Head Pressure @0.20psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @21:33:31.97 -> require 'vacuumCart' true @21:33:45.06 -> Power.skimmer :on @21:33:45.09 Gate.power :skimmer,:ON true @21:34:11.23 -> 3.times {refillSampleLines} @21:34:11.26 Refilling sample & bypass lines with fluid. @21:34:12.05 Toroid.seek :clear @21:34:12.27 Carousel.in :default @21:34:12.41 Carousel.home.seek 40 @21:34:13.21 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @21:34:25.42 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @21:34:25.44 Bypass.open @21:34:26.18 Intake.open @21:34:26.90 SamplePump.setPosition! 0ml @21:34:27.05 SamplePump.seek 30ml @21:35:04.27 Bypass.close @21:35:05.01 Exhaust.open @21:35:05.81 SamplePump.seek 55ml @21:35:36.94 delay 1 second @21:35:37.97 Intake.close @21:35:38.07 Exhaust.close @21:35:38.87 Sampling Loop filled with water @21:35:38.89 Refilling sample & bypass lines with fluid. @21:35:39.77 Toroid.seek :clear @21:35:40.01 Carousel.in :default @21:35:40.16 Carousel.home.seek 40 @21:35:40.48 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @21:35:40.52 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @21:35:40.54 Bypass.open @21:35:41.32 Intake.open @21:35:42.07 SamplePump.setPosition! 0ml @21:35:42.22 SamplePump.seek 30ml @21:36:19.45 Bypass.close @21:36:20.16 Exhaust.open @21:36:20.89 SamplePump.seek 55ml @21:36:52.02 delay 1 second @21:36:53.05 Intake.close @21:36:53.16 Exhaust.close @21:36:53.90 Sampling Loop filled with water @21:36:53.92 Refilling sample & bypass lines with fluid. @21:36:54.75 Toroid.seek :clear @21:36:54.93 Carousel.in :default @21:36:55.08 Carousel.home.seek 40 @21:36:55.37 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @21:36:55.41 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @21:36:55.43 Bypass.open @21:36:56.15 Intake.open @21:36:56.84 SamplePump.setPosition! 0ml @21:36:56.98 SamplePump.seek 30ml @21:37:34.21 Bypass.close @21:37:34.91 Exhaust.open @21:37:35.65 SamplePump.seek 55ml @21:38:06.77 delay 1 second @21:38:07.81 Intake.close @21:38:07.90 Exhaust.close @21:38:08.61 Sampling Loop filled with water 3 @21:38:47.89 -> BACsampler.prime @21:38:47.99 Priming sample loop w/150ml, bypass w/50ml @21:38:48.01 SamplePump.setPosition! 0ml @21:38:48.10 Bypass.open @21:38:49.61 Intake.open @21:38:50.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:38:53.04 Vacuum tare is 52% of full scale at 27C (drifted 1%) @21:38:53.21 Abs.in :default @21:38:53.29 Abs.hold @21:38:53.39 PhaseSeparator.in :full @21:38:53.47 PhaseSeparator.hold @21:38:53.72 Gate.power :skimmer,:OFF @21:38:53.86 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:38:53.99 SamplePump.stop @21:38:54.14 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.21psi @21:38:54.17 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @21:38:54.23 PhaseSeparator.in :filling @21:38:54.31 PhaseSeparator.hold @21:38:54.43 Gate.power :sampler,:ON @21:39:00.87 Head pressure reached 1.85psi in 7 seconds @21:39:00.89 Abs.hold @21:39:01.01 PhaseSeparator.in :full @21:39:01.09 PhaseSeparator.hold @21:39:01.34 Waiting for sampler to finish priming... @21:39:01.35 delayUntil "21:40:24.45" @21:40:24.53 SamplePump.in :start @21:40:24.67 SamplePump.seek 50ml,3:50 @21:40:25.21 SamplePump.configure :prime @21:40:27.78 SamplePump.stop @21:40:27.89 Intake.close @21:40:28.00 Bypass.close @21:40:28.86 Abs.stop @21:40:28.96 PhaseSeparator.stop @21:40:29.06 Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.2ml @21:40:56.79 -> BACsampler.source :sampler @21:41:28.21 -> Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.2ml @21:41:28.23 SyntaxError in ben -- compile error ben:10: syntax error, unexpected kIN Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall ... ^ ben:10: syntax error, unexpected tCONSTANT, expecting kDO or '{' or '(' ...::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made onl... ^ ben:10: syntax error, unexpected tIDENTIFIER, expecting $end ...erious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum aft... ^ @21:41:59.32 -> BACsampler.source = skimmer :skimmer @21:42:08.08 -> BACsampler.prime @21:42:08.18 Priming sample loop w/150ml, bypass w/50ml @21:42:08.20 SamplePump.setPosition! 0ml @21:42:08.28 Bypass.open @21:42:09.77 Intake.open @21:42:10.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:42:13.17 Vacuum tare is 52% of full scale at 27C (drifted 1%) @21:42:13.36 Abs.in :default @21:42:13.43 Abs.hold @21:42:13.53 PhaseSeparator.hold @21:42:13.78 Gate.power :sampler,:OFF @21:42:13.83 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:42:14.11 SamplePump.stop @21:42:14.26 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.12psi @21:42:14.29 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @21:42:14.35 PhaseSeparator.in :filling @21:42:14.43 PhaseSeparator.hold @21:42:14.55 Gate.power :skimmer,:ON @21:42:29.60 Gate.power :skimmer,:OFF @21:42:29.98 Gate.power :skimmer,:ON @21:42:45.02 Gate.power :skimmer,:OFF @21:42:45.39 Gate.power :skimmer,:ON @21:43:00.42 Gate.power :skimmer,:OFF @21:43:00.80 Gate.power :skimmer,:ON @21:43:15.83 Gate.power :skimmer,:OFF @21:43:16.21 Gate.power :skimmer,:ON @21:43:31.25 Gate.power :skimmer,:OFF @21:43:31.62 Gate.power :skimmer,:ON @21:43:46.65 Gate.power :skimmer,:OFF @21:43:47.04 Gate.power :skimmer,:ON @21:44:02.08 Gate.power :skimmer,:OFF @21:44:02.46 Gate.power :skimmer,:ON @21:44:14.69 PhaseSeparator.stop @21:44:14.77 Abs.stop @21:44:14.85 Cartridge::Sampler::HeadPressureTooLow in ben -- Only reached 0.08psi in 2:01 @21:45:40.19 Session Begun @21:45:45.20 -> showlog -100 @21:47:19.45 -> Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum after 2.2ml @21:47:19.48 SyntaxError in ben -- compile error ben:13: syntax error, unexpected kIN Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall ... ^ ben:13: syntax error, unexpected tCONSTANT, expecting kDO or '{' or '(' ...::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made onl... ^ ben:13: syntax error, unexpected tIDENTIFIER, expecting $end ...erious Leak or Stall -- 0.82ml/s made only 0.3psi vacuum aft... ^ @21:49:06.16 -> showlog -200 @21:50:14.52 -> BACsampler.sourvce @21:50:14.57 NoMethodError in nadia -- undefined method `sourvce' for BACsampler:Cartridge::Sampler @21:50:17.65 -> BACsampler.source :skimmer @21:50:29.30 -> BACsampler.prime @21:50:29.43 Priming sample loop w/150ml, bypass w/50ml @21:50:29.46 SamplePump.setPosition! 0ml @21:50:30.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:50:33.03 Vacuum tare is 52% of full scale at 27C (drifted 1%) @21:50:33.35 Abs.in :default @21:50:33.45 Abs.hold @21:50:33.57 PhaseSeparator.in :full @21:50:33.67 PhaseSeparator.hold @21:50:33.95 SamplePump.in :start @21:50:34.12 SamplePump.stop @21:50:34.28 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.08psi @21:50:34.33 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @21:50:34.43 PhaseSeparator.in :filling @21:50:34.52 PhaseSeparator.hold @21:50:34.69 SamplePump.configure :prime @21:50:34.78 Gate.power :skimmer,:OFF @21:50:35.20 Gate.power :skimmer,:ON @21:50:50.23 Gate.power :skimmer,:OFF @21:50:50.62 Gate.power :skimmer,:ON @21:51:05.65 Gate.power :skimmer,:OFF @21:51:06.03 Gate.power :skimmer,:ON @21:51:21.08 Gate.power :skimmer,:OFF @21:51:21.47 Gate.power :skimmer,:ON @21:51:36.50 Gate.power :skimmer,:OFF @21:51:36.92 Gate.power :skimmer,:ON @21:51:51.96 Gate.power :skimmer,:OFF @21:51:52.34 Gate.power :skimmer,:ON @21:52:07.37 Gate.power :skimmer,:OFF @21:52:07.76 Gate.power :skimmer,:ON @21:52:22.79 Gate.power :skimmer,:OFF @21:52:23.17 Gate.power :skimmer,:ON @21:52:34.84 PhaseSeparator.stop @21:52:34.99 Abs.stop @21:52:35.09 Cartridge::Sampler::HeadPressureTooLow in ben -- Only reached 0.05psi in 2:01 @21:55:48.59 -> require 'vacuumCart' false @21:56:09.21 -> require 'vacuumCart' false @21:56:22.19 -> Power.sampler :on @21:56:22.22 Gate.power :sampler,:ON true @21:56:38.52 -> BACsampler.source = sampler :sampler @21:56:55.57 -> BACsampler.prime @21:56:55.70 Priming sample loop w/150ml, bypass w/50ml @21:56:55.73 SamplePump.setPosition! 0ml @21:56:56.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:56:59.33 Vacuum tare is 52% of full scale at 27C (drifted 1%) @21:56:59.53 Abs.hold @21:56:59.66 PhaseSeparator.in :full @21:56:59.75 PhaseSeparator.hold @21:57:00.01 Waiting for sampler to finish priming... @21:57:00.03 delayUntil "21:57:52.27" @21:57:00.15 SamplePump.stop @21:57:00.32 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.08psi @21:57:00.37 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @21:57:00.45 PhaseSeparator.in :filling @21:57:00.56 PhaseSeparator.hold @21:57:00.76 Gate.power :sampler,:OFF @21:57:01.15 Gate.power :sampler,:ON @21:57:16.18 Gate.power :sampler,:OFF @21:57:16.56 Gate.power :sampler,:ON @21:57:31.61 Gate.power :sampler,:OFF @21:57:31.99 Gate.power :sampler,:ON @21:57:47.03 Gate.power :sampler,:OFF @21:57:47.41 Gate.power :sampler,:ON @21:58:02.44 Gate.power :sampler,:OFF @21:58:02.83 Gate.power :sampler,:ON @21:58:17.87 Gate.power :sampler,:OFF @21:58:18.25 Gate.power :sampler,:ON @21:58:33.29 Gate.power :sampler,:OFF @21:58:33.68 Gate.power :sampler,:ON @21:58:48.71 Gate.power :sampler,:OFF @21:58:49.10 Gate.power :sampler,:ON @21:59:00.87 PhaseSeparator.stop @21:59:00.95 Abs.stop @21:59:01.70 Cartridge::Sampler::HeadPressureTooLow in ben -- Only reached 0.05psi in 2:01 @22:01:01.83 -> BACsampler.source = sampler :sampler @22:01:20.77 -> BACsampler.prime @22:01:20.91 Priming sample loop w/150ml, bypass w/50ml @22:01:20.92 SamplePump.setPosition! 0ml @22:01:21.88 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:01:24.50 Vacuum tare is 52% of full scale at 27C (drifted 1%) @22:01:24.73 Abs.in :default @22:01:24.83 Abs.hold @22:01:24.94 PhaseSeparator.in :full @22:01:25.04 PhaseSeparator.hold @22:01:25.34 SamplePump.in :start @22:01:25.52 SamplePump.stop @22:01:25.68 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.05psi @22:01:25.74 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @22:01:25.82 PhaseSeparator.in :filling @22:01:25.93 PhaseSeparator.hold @22:01:26.06 Gate.power :sampler,:OFF @22:01:26.12 SamplePump.configure :prime @22:01:26.45 Gate.power :sampler,:ON @22:01:41.48 Gate.power :sampler,:OFF @22:01:41.86 Gate.power :sampler,:ON @22:01:56.90 Gate.power :sampler,:OFF @22:01:57.28 Gate.power :sampler,:ON @22:02:12.32 Gate.power :sampler,:OFF @22:02:12.70 Gate.power :sampler,:ON @22:02:27.73 Gate.power :sampler,:OFF @22:02:28.12 Gate.power :sampler,:ON @22:02:43.15 Gate.power :sampler,:OFF @22:02:43.53 Gate.power :sampler,:ON @22:02:58.57 Gate.power :sampler,:OFF @22:02:59.01 Gate.power :sampler,:ON @22:03:07.22 -> head :head @22:03:11.61 -> Head Intake Head Pressure @0.06psi @22:03:14.04 Gate.power :sampler,:OFF @22:03:14.43 Gate.power :sampler,:ON @22:03:26.18 PhaseSeparator.stop @22:03:26.27 Abs.stop @22:03:26.37 Cartridge::Sampler::HeadPressureTooLow in ben -- Only reached 0.05psi in 2:01 @22:14:58.42 -> BACsampler.source = sampler :sampler @22:15:07.53 -> BACsampler.prime @22:15:07.66 Priming sample loop w/150ml, bypass w/50ml @22:15:07.69 SamplePump.setPosition! 0ml @22:15:08.71 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:15:11.32 Vacuum tare is 52% of full scale at 27C (drifted 1%) @22:15:11.53 Abs.in :default @22:15:11.62 Abs.hold @22:15:11.74 PhaseSeparator.in :full @22:15:11.83 PhaseSeparator.hold @22:15:12.14 SamplePump.in :start @22:15:12.31 SamplePump.stop @22:15:12.48 Cartridge::Sampler::LostHeadPressure in ben -- Head Pressure Fell to 0.09psi @22:15:12.51 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @22:15:12.66 PhaseSeparator.in :filling @22:15:12.76 PhaseSeparator.hold @22:15:12.89 Gate.power :sampler,:OFF @22:15:12.96 SamplePump.configure :prime @22:15:13.28 Gate.power :sampler,:ON @22:15:28.31 Gate.power :sampler,:OFF @22:15:28.70 Gate.power :sampler,:ON @22:15:43.74 Gate.power :sampler,:OFF @22:15:44.12 Gate.power :sampler,:ON @22:15:59.15 Gate.power :sampler,:OFF @22:15:59.53 Gate.power :sampler,:ON @22:16:14.58 Gate.power :sampler,:OFF @22:16:15.00 Gate.power :sampler,:ON @22:16:30.03 Gate.power :sampler,:OFF @22:16:30.42 Gate.power :sampler,:ON @22:16:45.45 Gate.power :sampler,:OFF @22:16:45.83 Gate.power :sampler,:ON @22:17:00.87 Gate.power :sampler,:OFF @22:17:01.25 Gate.power :sampler,:ON @22:17:13.00 PhaseSeparator.stop @22:17:13.10 Abs.stop @22:17:13.20 Cartridge::Sampler::HeadPressureTooLow in ben -- Only reached 0.05psi in 2:01 @22:19:39.70 -> BACsampler.source = sampler :sampler @22:19:49.94 -> BACsampler.prime @22:19:50.08 Priming sample loop w/150ml, bypass w/50ml @22:19:50.11 SamplePump.setPosition! 0ml @22:19:51.77 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:19:54.39 Vacuum tare is 52% of full scale at 27C (drifted 0%) @22:19:54.63 Abs.in :default @22:19:54.74 Abs.hold @22:19:54.86 PhaseSeparator.in :full @22:19:54.94 PhaseSeparator.hold @22:19:55.27 SamplePump.in :start @22:19:55.38 SamplePump.seek 50ml,3:50 @22:19:55.96 SamplePump.configure :prime @22:20:49.67 SamplePump.stop @22:20:49.78 Intake.close @22:20:49.88 Bypass.close @22:20:50.79 Abs.stop @22:20:50.88 PhaseSeparator.stop @22:20:50.99 Cartridge::Sampler::Leaky in ben -- Serious Leak or Stall -- 0.82ml/s made only 0.2psi vacuum after 44.1ml @22:21:30.11 -> Power.sampler :off @22:21:30.13 Gate.power :sampler,:OFF false @22:21:48.99 -> Power.sampler :on @22:21:49.01 Gate.power :sampler,:ON true @22:22:04.97 -> Power.skimmer :off @22:22:04.99 Gate.power :skimmer,:OFF false @22:23:57.29 -> require 'vacuumCart' false @22:24:12.27 -> refillSampleLines @22:24:12.29 Refilling sample & bypass lines with fluid. @22:24:13.21 Toroid.seek :clear @22:24:13.43 Carousel.in :default @22:24:13.60 Carousel.home.seek 40 @22:24:13.90 Waiting for ben to answer "Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return." @22:26:19.54 Answered Hook Intake and Exhaust Lines to MillQ water. Open Pressure Interface Valve. Press Return."" @22:26:19.56 Bypass.open @22:26:20.33 Intake.open @22:26:21.07 SamplePump.setPosition! 0ml @22:26:21.22 SamplePump.in :default @22:26:21.32 SamplePump.seek 30ml @22:26:58.55 Bypass.close @22:26:59.40 Exhaust.open @22:27:00.14 SamplePump.seek 55ml @22:27:31.26 delay 1 second @22:27:32.30 Intake.close @22:27:32.37 Exhaust.close @22:27:33.10 Sampling Loop filled with water @22:27:51.45 -> BACsampler.prime @22:27:51.59 Priming sample loop w/150ml, bypass w/50ml @22:27:51.60 SamplePump.setPosition! 0ml @22:27:51.71 Bypass.open @22:27:53.23 Intake.open @22:27:54.04 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:27:56.69 Vacuum tare is 52% of full scale at 27C (drifted 1%) @22:27:56.89 Abs.in :default @22:27:56.98 Abs.hold @22:27:57.09 PhaseSeparator.hold @22:27:58.10 SamplePump.in :start @22:27:58.20 SamplePump.seek 50ml,3:50 @22:27:58.78 SamplePump.configure :prime @22:28:59.63 Abs.stop @22:28:59.79 PhaseSeparator.stop @22:28:59.87 SamplePump.setPosition! 0ml @22:28:59.96 Exhaust.open @22:29:01.36 Bypass.close @22:29:02.96 Abs.in :default @22:29:03.06 Abs.hold @22:29:03.17 PhaseSeparator.hold @22:29:03.54 SamplePump.in :start @22:29:03.65 SamplePump.seek 150ml,10:30 @22:29:04.23 SamplePump.configure :prime @22:32:07.13 Abs.stop @22:32:07.23 PhaseSeparator.stop BACsampler @22:34:17.36 -> deep; fire archive, lysis # @22:34:18.40 Toroid.seek :clear @22:34:18.64 Carousel.in :default @22:34:18.81 Carousel.home.seek 40 @22:34:19.14 Selecting Cartridge 49 @22:34:19.48 Carousel.seek 49 @22:34:21.69 Lysis1.in :fast @22:34:21.80 Lysis1.seek :locked @22:34:24.46 Plunge.seek :locked @22:34:24.71 Air.in :fast @22:34:24.81 Air.seek :locked @22:34:25.82 Carousel.reconfigure :locked @22:34:26.01 Powering cartridge bus @22:34:28.69 Gate.setIR 1750,5000,0,6 @22:34:28.83 Gate.setIR 0,5000,0,6 @22:34:28.94 Gate.power :cartridge,:OFF @22:34:29.53 Mounted archive Cartridge @22:34:29.65 BACsampler with {source: :sampler} @22:34:29.73 GoodNews.email "BACsampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 49" @22:34:30.07 Preparing to filter cartridge 49 @22:34:30.36 Gate.power :sampler,:OFF @22:34:30.39 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @22:34:51.60 upsync @22:35:18.54 Intake Head Pressure tare is 3% of full scale at 27C (drifted 2%) @22:35:18.55 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @22:35:18.69 PhaseSeparator.in :filling @22:35:18.78 PhaseSeparator.hold @22:35:18.90 Gate.power :sampler,:ON @22:35:23.35 Head pressure reached 1.91psi in 5 seconds @22:35:23.41 Bypass.open @22:35:24.16 Intake.close @22:35:25.01 Toroid.in :hold @22:35:25.12 Toroid.seek :crack @22:35:26.72 Toroid.seek :cartridge @22:35:27.88 Toroid.in :default @22:35:27.99 Toroid.seek :clear @22:35:30.09 Pressurizing cartridge to 26psi above 13.8psia @22:35:30.11 SamplePump.setPosition! 0ml @22:35:30.23 Intake.open @22:35:30.93 Bypass.close @22:35:31.64 Exhaust.close @22:35:33.20 Abs.in :default @22:35:33.31 Abs.hold @22:35:33.42 PhaseSeparator.in :full @22:35:33.54 PhaseSeparator.hold @22:35:33.80 Waiting for sampler to finish priming... @22:35:33.82 delayUntil "22:36:48.95" @22:36:49.00 SamplePump.in :charge @22:36:49.12 SamplePump.seek 2.3ml,42 seconds @22:36:58.64 Abs.stop @22:36:58.75 PhaseSeparator.stop @22:36:58.87 Intake.close @22:36:59.67 Pressurized cartridge with 0.400ml to 26.1psi @22:36:59.69 Isolated @38.1psia (24.3psi) @22:36:59.74 Verifying cartridge holds at least 0.65..0.85 of 38.1psia @22:36:59.83 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @22:37:04.46 Cartridge pressure fell 1% to 37.8psia @22:37:04.64 Sampling 1000.0ml @22:37:04.66 SamplePump.setPosition! 0ml @22:37:04.80 Exhaust.open @22:37:05.45 Intake.open @22:37:06.38 Abs.in :default @22:37:06.48 Abs.hold @22:37:06.64 PhaseSeparator.hold @22:37:07.04 SamplePump.in :start @22:37:07.15 SamplePump.seek 1000ml,1:23:50 @22:37:07.72 SamplePump.configure :sample @22:37:10.13 Cartridge 49 filtering 2/1000ml at 0.64ml/s with 27.9psi vacuum @22:37:57.46 -> exit @22:37:57.49 Session Ended @22:38:10.17 Cartridge 49 filtering 39/1000ml at 0.62ml/s with 22.9psi vacuum @22:39:10.19 Cartridge 49 filtering 75/1000ml at 0.60ml/s with 15.8psi vacuum @22:40:10.18 Cartridge 49 filtering 110/1000ml at 0.58ml/s with 20.9psi vacuum @22:41:10.18 Cartridge 49 filtering 143/1000ml at 0.56ml/s with 19.3psi vacuum @22:42:10.19 Cartridge 49 filtering 176/1000ml at 0.54ml/s with 19.7psi vacuum @22:43:10.18 Cartridge 49 filtering 207/1000ml at 0.52ml/s with 16.0psi vacuum @22:44:10.18 Cartridge 49 filtering 237/1000ml at 0.51ml/s with 26.7psi vacuum @22:45:10.18 Cartridge 49 filtering 267/1000ml at 0.49ml/s with 25.0psi vacuum @22:46:10.18 Cartridge 49 filtering 296/1000ml at 0.48ml/s with 23.3psi vacuum @22:47:10.77 Cartridge 49 filtering 323/1000ml at 0.46ml/s with 25.6psi vacuum @22:48:10.18 Cartridge 49 filtering 349/1000ml at 0.44ml/s with 23.4psi vacuum @22:49:10.18 Cartridge 49 filtering 374/1000ml at 0.42ml/s with 28.6psi vacuum @22:50:10.18 Cartridge 49 filtering 398/1000ml at 0.39ml/s with 18.9psi vacuum @22:51:10.18 Cartridge 49 filtering 419/1000ml at 0.36ml/s with 21.1psi vacuum @22:52:10.18 Cartridge 49 filtering 439/1000ml at 0.33ml/s with 23.1psi vacuum @22:53:10.18 Cartridge 49 filtering 458/1000ml at 0.31ml/s with 27.1psi vacuum @22:54:10.18 Cartridge 49 filtering 475/1000ml at 0.28ml/s with 21.3psi vacuum @22:55:10.18 Cartridge 49 filtering 491/1000ml at 0.26ml/s with 24.5psi vacuum @22:56:10.18 Cartridge 49 filtering 505/1000ml at 0.24ml/s with 20.5psi vacuum @22:57:10.18 Cartridge 49 filtering 518/1000ml at 0.21ml/s with 21.5psi vacuum @22:58:10.18 Cartridge 49 filtering 529/1000ml at 0.19ml/s with 21.7psi vacuum @22:58:10.26 SamplePump.stop @22:58:10.35 Intake.close @22:58:10.42 Exhaust.close @22:58:10.88 Toroid.in :hold @22:58:10.97 Toroid.seek :bypass @22:58:12.67 Toroid.in :default @22:58:12.77 Toroid.seek :clear @22:58:14.42 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @22:58:14.44 Abs.stop @22:58:14.55 PhaseSeparator.stop @22:58:14.84 Sampled 528.9ml @22:58:15.36 GoodNews.email "After filtering 528.9ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 49" @22:58:15.90 Plunge.seek :reagent @22:58:16.74 Sample loop closed. Ready to Deliver Reagents @22:58:16.77 Archive.seek :sealed @22:58:17.99 Gate.power :sampler,:OFF @22:58:20.46 upsync @22:58:20.79 Archive.seek :evac @22:58:35.06 delay 1:00 @22:59:35.10 Archive.seek :clear @22:59:52.83 RNAlater soak for 10:00 @23:09:52.87 Archive.seek :tVent @23:09:55.44 delay 1 second @23:09:56.47 Archive.seek :evac @23:10:12.14 delay 10 seconds @23:10:22.17 Archive.seek :tVent @23:10:37.85 delay 1 second @23:10:38.88 Archive.seek :evac @23:10:54.55 delay 10 seconds @23:11:04.60 Sample preserved and evacuated @23:11:04.79 Selecting Cartridge 38 @23:11:05.05 Carousel.in :default @23:11:05.19 Carousel.home.seek 49 @23:11:05.55 Archive.in :unlocking @23:11:05.71 Archive.seek :clear @23:11:05.81 Air.in :unlocking @23:11:06.00 Air.seek :clear @23:11:10.30 Lysis1.in :unlocking @23:11:10.48 Lysis1.seek :clear @23:11:10.66 Plunge.in :unlocking @23:11:10.74 Plunge.seek :clear @23:11:12.18 Carousel.seek 38 @23:11:14.80 Lysis1.in :fast @23:11:14.88 Lysis1.seek :locked @23:11:17.54 Plunge.in :default @23:11:17.67 Plunge.seek :locked @23:11:17.82 Air.in :fast @23:11:17.91 Air.seek :locked @23:11:18.91 Carousel.reconfigure :locked @23:11:19.09 Powering cartridge bus @23:11:21.76 Gate.setIR 1750,5000,0,6 @23:11:21.90 Gate.setIR 0,5000,0,6 @23:11:22.00 PuckHeater.configure :default @23:11:22.09 Gate.power :heat,:ON,limit to 5000mA @23:11:22.92 Mounted lysis Cartridge board#Q05044 @23:11:22.99 Using this cartridge's heat loss curve for HeatingAccelInCarousel @23:11:23.04 PhytoSampler with {source: :sampler} @23:11:23.08 GoodNews.email "PhytoSampler with {source: :sampler} filtering up to 1000 ml",Subject: "Sampling microSPR_phyto cartridge in slot 38" @23:11:23.47 Preparing to filter cartridge 38 @23:11:23.81 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @23:11:28.80 upsync @23:11:42.93 Intake Head Pressure tare is 3% of full scale at 27C (drifted 2%) @23:11:42.95 Waiting up to 2:00 for Intake Head Pressure to reach 0.70psi @23:11:43.03 PhaseSeparator.in :filling @23:11:43.10 PhaseSeparator.hold @23:11:43.21 Gate.power :sampler,:ON @23:11:49.66 Head pressure reached 1.94psi in 7 seconds @23:11:49.68 Air.in :default @23:11:49.88 Priming sample loop w/150ml, bypass w/50ml @23:11:49.90 Air.seek :sealed @23:11:49.96 SamplePump.setPosition! 0ml @23:11:50.10 Bypass.open @23:11:51.60 Intake.open @23:11:52.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:11:55.04 Vacuum tare is 52% of full scale at 27C (drifted 1%) @23:11:55.33 Abs.in :default @23:11:55.41 Abs.hold @23:11:55.53 PhaseSeparator.in :full @23:11:55.61 PhaseSeparator.hold @23:11:55.87 Waiting for sampler to finish priming... @23:11:55.89 delayUntil "23:13:13.25" @23:13:13.32 SamplePump.in :start @23:13:13.43 SamplePump.seek 50ml,3:50 @23:13:14.01 SamplePump.configure :prime @23:14:14.82 Abs.stop @23:14:14.91 PhaseSeparator.stop @23:14:15.00 SamplePump.setPosition! 0ml @23:14:15.08 Exhaust.open @23:14:16.44 Bypass.close @23:14:18.01 Abs.in :default @23:14:18.10 Abs.hold @23:14:18.21 PhaseSeparator.hold @23:14:18.56 SamplePump.in :start @23:14:18.71 SamplePump.seek 150ml,10:30 @23:14:19.26 SamplePump.configure :prime @23:17:21.95 Abs.stop @23:17:22.08 PhaseSeparator.stop @23:17:22.19 Bypass.open @23:17:22.96 Intake.close @23:17:23.78 Toroid.in :hold @23:17:23.87 Toroid.seek :crack @23:17:25.44 Toroid.seek :cartridge @23:17:26.61 Toroid.in :default @23:17:26.72 Toroid.seek :clear @23:17:28.82 Pressurizing cartridge to 26psi above 13.5psia @23:17:28.84 SamplePump.setPosition! 0ml @23:17:28.94 Intake.open @23:17:29.64 Bypass.close @23:17:30.33 Exhaust.close @23:17:31.11 Abs.in :default @23:17:31.20 Abs.hold @23:17:31.31 PhaseSeparator.hold @23:17:31.59 SamplePump.in :charge @23:17:31.71 SamplePump.seek 2.3ml,42 seconds @23:17:45.93 Abs.stop @23:17:46.47 PhaseSeparator.stop @23:17:46.71 Intake.close @23:17:47.51 Pressurized cartridge with 0.907ml to 26.2psi @23:17:47.52 Isolated @37.8psia (24.2psi) @23:17:47.56 Verifying cartridge holds at least 0.65..0.85 of 37.8psia @23:17:47.65 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:17:52.33 Sampling 1000.0ml @23:17:52.35 SamplePump.setPosition! 0ml @23:17:52.45 Exhaust.open @23:17:53.09 Intake.open @23:17:54.00 Abs.in :default @23:17:54.09 Abs.hold @23:17:54.19 PhaseSeparator.hold @23:17:54.56 SamplePump.in :start @23:17:54.70 SamplePump.seek 1000ml,1:23:50 @23:17:55.24 SamplePump.configure :sample @23:17:57.66 Cartridge 38 filtering 2/1000ml at 0.62ml/s with 21.1psi vacuum @23:18:57.72 Cartridge 38 filtering 40/1000ml at 0.64ml/s with 22.9psi vacuum @23:19:57.71 Cartridge 38 filtering 77/1000ml at 0.62ml/s with 18.8psi vacuum 4.3m deep (21.0psia) @23:20:57.70 Cartridge 38 filtering 113/1000ml at 0.60ml/s with 22.3psi vacuum @23:21:57.71 Cartridge 38 filtering 148/1000ml at 0.58ml/s with 27.8psi vacuum @23:22:57.71 Cartridge 38 filtering 181/1000ml at 0.56ml/s with 24.2psi vacuum @23:23:57.70 Cartridge 38 filtering 214/1000ml at 0.54ml/s with 18.7psi vacuum @23:24:57.70 Cartridge 38 filtering 246/1000ml at 0.53ml/s with 27.5psi vacuum @23:25:57.71 Cartridge 38 filtering 276/1000ml at 0.51ml/s with 26.7psi vacuum @23:26:57.70 Cartridge 38 filtering 306/1000ml at 0.49ml/s with 24.0psi vacuum @23:27:57.70 Cartridge 38 filtering 335/1000ml at 0.48ml/s with 28.9psi vacuum @23:28:57.71 Cartridge 38 filtering 362/1000ml at 0.45ml/s with 18.4psi vacuum @23:29:57.70 Cartridge 38 filtering 387/1000ml at 0.42ml/s with 23.7psi vacuum @23:30:57.70 Cartridge 38 filtering 411/1000ml at 0.40ml/s with 25.0psi vacuum @23:31:57.71 Cartridge 38 filtering 433/1000ml at 0.36ml/s with 23.2psi vacuum @23:32:57.71 Cartridge 38 filtering 453/1000ml at 0.34ml/s with 24.8psi vacuum @23:33:57.71 Cartridge 38 filtering 472/1000ml at 0.31ml/s with 27.1psi vacuum @23:34:58.17 Cartridge 38 filtering 489/1000ml at 0.29ml/s with 24.9psi vacuum @23:35:57.71 Cartridge 38 filtering 504/1000ml at 0.25ml/s with 24.4psi vacuum @23:36:57.71 Cartridge 38 filtering 518/1000ml at 0.23ml/s with 26.2psi vacuum @23:37:57.71 Cartridge 38 filtering 530/1000ml at 0.20ml/s with 23.6psi vacuum @23:38:57.71 Cartridge 38 filtering 541/1000ml at 0.18ml/s with 23.7psi vacuum @23:38:57.79 SamplePump.stop @23:38:57.88 Intake.close @23:38:57.96 Exhaust.close @23:38:58.27 Toroid.in :hold @23:38:58.37 Toroid.seek :bypass @23:39:00.10 Toroid.in :default @23:39:00.19 Toroid.seek :clear @23:39:01.84 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @23:39:01.87 Abs.stop @23:39:01.96 PhaseSeparator.stop @23:39:02.23 Sampled 541.2ml @23:39:02.74 GoodNews.email "After filtering 541.2ml",Subject: "Processing microSPR_phyto cartridge in slot 38" @23:39:04.47 SPR.command "read_sequences" @23:39:04.85 SPR.command "stats_on" @23:39:05.04 SPR.command "start 0 1" @23:39:05.39 Gate.power :sampler,:OFF @23:39:05.75 SPR.start /var/log/esp/spr24aug0823h.spr.bz2 @23:39:07.70 Handoff.select :waste @23:39:07.91 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:39:08.05 upsync @23:39:14.55 Syringe tare is 52% of full scale at 27C (drifted 1%) @23:39:14.62 PinPort.in :locking @23:39:14.75 PinPort.seek :locked @23:39:15.31 Archive.in :fast @23:39:15.42 Lysis2.in :fast @23:39:15.49 Lysis1.seek 1350uL @23:39:15.56 Archive.seek :full @23:39:15.78 Lysis2.seek 950uL @23:39:20.66 Plunge.seek :reagent @23:39:21.60 Evacuating puck with filter bubble point of 24psi @23:39:21.63 PinPort.seek :locked @23:39:21.77 Handoff.select :waste @23:39:21.81 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:39:28.44 Air syringe pumping up to 1 strokes to reach 8psi @23:39:28.45 Air stroke #1 of 1 @23:39:28.63 Air.in :pressurize @23:39:28.74 Air.seek :pressurized @23:39:34.38 1.0psi after pressurize @23:39:34.41 Air.in :compress @23:39:34.52 Air.seek :evac @23:39:37.64 3.1psi after compress @23:39:55.74 0.3psi after compress (stable) @23:39:55.76 Air.in :default @23:39:55.87 Air.hold @23:39:56.13 at 0.2psi Opened to waste @23:39:56.14 Handoff.select :blocked @23:39:56.32 Archive syringe pumping up to 8 strokes to reach 24psi @23:39:56.34 Archive stroke #1 of 8 @23:39:56.51 Archive.in :pressurize @23:39:56.63 Archive.seek :pressurized @23:40:09.10 2.5psi after pressurize @23:40:14.94 2.3psi after pressurize (stable) @23:40:15.06 Archive.in :compress @23:40:15.18 Archive.seek :empty @23:40:30.95 19.9psi after compress @23:40:42.55 21.7psi after compress (stable) @23:40:42.59 Archive.in :gulp @23:40:42.72 Archive.seek :topVent @23:40:49.11 22.7psi after gulp @23:40:54.71 22.1psi after gulp (stable) @23:40:54.73 Archive stroke #2 of 8 @23:40:54.88 Archive.in :pressurize @23:40:54.96 Archive.seek :pressurized @23:41:07.48 22.6psi after pressurize @23:41:13.12 22.0psi after pressurize (stable) @23:41:13.16 Archive.in :compress @23:41:13.27 Archive.seek :empty @23:41:17.90 Reached 24.1 of 24.0psi after pumping 3220uL (1.86 strokes) @23:41:17.92 Archive.in :default @23:41:18.02 Archive.hold @23:41:18.15 Handoff.select :waste @23:41:18.31 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:41:48.91 delay 1:00 @23:42:48.93 Handoff.select :blocked @23:42:49.10 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @23:43:11.70 Handoff.select :waste @23:43:11.90 0.5psi at evacEnd @23:43:11.92 Handoff.select :blocked @23:43:12.14 Archive.in :fast @23:43:12.26 Archive.seek :topVent @23:43:18.65 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @23:43:25.25 Stable @0.5psi after Handoff blocked @23:43:25.28 Archive syringe pumping up to 8 strokes to reach 24psi @23:43:25.32 Archive stroke #1 of 8 @23:43:25.49 Archive.in :pressurize @23:43:25.60 Archive.seek :pressurized @23:43:38.08 1.1psi after pressurize @23:43:38.11 Archive.in :compress @23:43:38.21 Archive.seek :empty @23:43:53.96 15.5psi after compress @23:44:04.56 15.1psi after compress (stable) @23:44:04.63 Archive.in :gulp @23:44:04.74 Archive.seek :topVent @23:44:11.08 16.2psi after gulp @23:44:16.68 15.5psi after gulp (stable) @23:44:16.70 Archive stroke #2 of 8 @23:44:17.31 Archive.in :pressurize @23:44:17.52 Archive.seek :pressurized @23:44:30.00 15.9psi after pressurize @23:44:30.03 Archive.in :compress @23:44:30.14 Archive.seek :empty @23:44:41.99 Reached 24.6 of 24.5psi after pumping 3376uL (1.95 strokes) @23:44:42.01 Archive.in :default @23:44:42.16 Archive.hold @23:44:42.29 Handoff.select :waste @23:44:42.45 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:44:50.04 delay 1:00 @23:45:50.07 Handoff.select :blocked @23:45:50.25 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @23:46:12.89 Handoff.select :waste @23:46:13.07 0.3psi at evacEnd @23:46:13.10 Air.seek :fullVent @23:46:14.47 Archive.in :fast @23:46:14.56 Archive.seek :topVent @23:46:21.50 Seawater evacuated, Pressure above/below filter released. @23:46:21.63 Lysis1.in :slow @23:46:21.73 Lysis1.seek 1050uL @23:46:51.39 delay 5 seconds @23:46:56.41 Handoff.select :blocked @23:46:56.68 Lysis1.seek 450uL @23:47:55.85 delay 3 seconds @23:47:58.95 Air.in mix with 1.57127 parts Lysis1 @23:47:59.65 Air.seek :evac @23:47:59.71 Lysis1.seek 300uL @23:48:14.74 Lysis1.seek :empty @23:48:44.47 Lysis buffer delivered @26.1psi @23:48:44.49 Polling Can every 2:00 @23:48:45.06 PuckHeater.configure :default #ambient 27.3C @23:48:45.17 PuckHeater.seek 80.4C @23:49:40.71 PuckHeater.hold 2:00,80.4C @23:51:40.77 Polling Can every 2:00 @23:51:41.32 PuckHeater.configure :default #ambient 27.3C @23:51:41.46 PuckHeater.hold 30 seconds,79.4C @23:52:11.67 Polling Can every 2:00 @23:52:12.26 PuckHeater.configure :default #ambient 27.3C @23:52:12.50 PuckHeater.hold 30 seconds,78.8C @23:52:12.85 Session Begun @23:52:16.04 -> can Can@23:52:15, 27.3C, 46% humidity, 14.2psia, 14.810V, 0.860A, 3.194Ah, 12.74W @23:52:33.48 -> exit @23:52:33.52 Session Ended @23:52:42.90 Polling Can every 2:00 @23:52:43.46 PuckHeater.configure :default #ambient 27.3C @23:52:43.60 PuckHeater.hold 10:00,78.3C @00:01:11.08UTC09-Aug-24 Session Begun @00:01:14.61 -> showlog @00:02:43.03 Lysis complete. Syringe pressure @34.8psi @00:02:43.07 Archive syringe pumping up to 5 strokes to reach 40psi @00:02:43.10 Archive stroke #1 of 5 @00:02:43.34 Archive.in :pressurize @00:02:43.46 Archive.seek :pressurized @00:02:55.94 34.6psi after pressurize @00:03:01.54 34.0psi after pressurize (stable) @00:03:01.57 Archive.in :compress @00:03:01.71 Archive.seek :empty @00:03:10.35 Reached 43.6 of 40.0psi after pumping 1577uL (0.91 strokes) @00:03:10.38 Archive.in :default @00:03:10.49 Archive.hold @00:03:10.71 delay 2 seconds @00:03:12.74 Archive.in :fast @00:03:12.89 Archive.seek :full @00:03:19.64 Lysis1.seek 138uL @00:03:33.38 delay 1 second @00:03:34.42 Archive.seek :full @00:03:34.46 Lysis1.seek :empty @00:03:48.36 Handoff.select :blocked @00:03:48.63 Initial air pressure @47.5psi @00:03:48.77 Lysis1.in :extract @00:03:48.86 Lysis1.seek 600uL,nil @00:03:52.05 Archive syringe pumping up to 6 strokes to reach 25psi @00:03:52.07 Archive stroke #1 of 6 @00:03:52.30 Archive.in :pressurize @00:03:52.37 Archive.seek :pressurized @00:04:04.87 12.6psi after pressurize @00:04:10.48 11.7psi after pressurize (stable) @00:04:10.51 Archive.in :compress @00:04:10.69 Archive.seek :empty @00:04:17.82 Archive.in :default @00:04:17.89 Archive.hold @00:04:18.15 Initial 600uL lysate @18.4psi @00:04:18.17 Handoff.select :blocked @00:04:18.35 Initial air pressure @18.0psi @00:04:18.49 Lysis1.seek 780uL,nil @00:04:19.97 Archive syringe pumping up to 6 strokes to reach 17psi @00:04:19.98 Archive stroke #1 of 6 @00:04:20.16 Archive.in :compress @00:04:20.29 Archive.seek :empty @00:04:21.33 Archive.in :default @00:04:21.39 Archive.hold @00:04:21.76 Recovered 780uL lysate @16.3psi @00:04:21.82 Air.in :halfSpeed @00:04:21.94 Air.seek :fullVent @00:04:23.97 Puck Top Vented, bottom @18.3psi @00:04:23.99 Handoff.select :waste @00:04:24.20 delay 5 seconds @00:04:29.29 Handoff open to waste, bottom @0.9psi @00:04:29.32 Air.in :default @00:04:29.45 Air.seek :evac @00:04:30.83 Lysis2.in :DAmix @00:04:31.07 Lysis1.in :DAmix @00:04:31.76 Lysis2.seek 700uL @00:04:31.86 Lysis1.seek 530uL @00:05:06.49 delay 5 seconds @00:05:11.51 Handoff.select SPR @00:05:11.71 SPR.command "start 2 14" @00:05:13.58 SPR stabilized @00:05:13.77 delay 10 seconds @00:05:23.80 SPR.command "start 2 0" @00:05:26.42 Waiting up to 11:00 for SPR to enter state 1 @00:07:24.10 SPR.command "hold_on" @00:07:25.49 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:07:26.28 Lysis2.seek 400uL @00:07:26.38 Lysis1.seek 230uL @00:08:07.88 delay 15 seconds @00:08:22.91 SPR.command "hold_off" @00:08:26.14 Waiting up to 1:00:00 for SPR to enter state 7 @00:17:55.82 Session Ended @00:24:41.72 SPRsummary:-4.83RIU,168RIU,86RIU,4.06RIU,none,13ng/L,high after 2890s @00:38:58.65 SPR analysis completed @00:39:07.79 Ended log spr24aug0823h.spr.bz2 @00:39:07.81 SPR.command "stats_off" @00:39:08.71 Handoff.select :close @00:39:08.87 Gate.power :heat,:OFF @00:39:08.89 Gate.power :cartridge,:OFF @00:39:09.01 PinPort.in :unlocking @00:39:09.10 PinPort.seek :clear @00:39:09.60 Carousel.in :default @00:39:09.75 Carousel.home.seek 38 @00:39:10.11 Plunge.seek :locked @00:39:11.04 Archive.in :unlocking @00:39:11.21 Archive.seek :clear @00:39:11.29 Air.in :unlocking @00:39:11.57 Air.seek :clear @00:39:11.60 Lysis1.in :unlocking @00:39:11.87 Lysis1.seek :clear @00:39:11.94 Lysis2.in :unlocking @00:39:12.11 Lysis2.seek :clear @00:39:16.15 Gate.power :analytic1,:OFF @00:39:16.17 Gate.power :motor,:OFF @00:39:16.22 Gate.power :core,:OFF @00:39:16.25 Gate.power :main,:OFF @00:39:16.78 Safely stopped and ready to power off @00:52:45.70 Session Begun @00:52:48.42 -> showlog @00:52:54.40 -> exit @00:52:54.43 Session Ended @18:21:31.29UTC14-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @18:21:33.06 >BREAK< ESP3 Gateway v1.43 for core bus @18:21:33.08 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:21:36.94 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @18:21:37.64 Sea pressure (Abs) tare is 6% of full scale @18:21:50.70 Polling Can every 2:00 @18:21:50.75 Waiting for espclients... @18:21:51.48 Session Begun @18:21:53.11 Session Begun @18:21:57.34 -> ESP.ready! @18:21:57.59 Gate.power :sampler,:OFF,limit to 3500mA @18:21:57.62 Gate.power :skimmer,:OFF,limit to 3500mA @18:21:57.68 Gate.power :analytic1,:ON,limit to 4000mA @18:21:58.42 Plunge.configure :default @18:21:58.51 Plunge.hold @18:21:58.64 Plunge.configure :default @18:21:58.88 SamplePump.configure :default @18:21:59.08 Toroid.configure :default @18:21:59.18 Toroid.hold @18:21:59.31 Toroid.configure :default @18:21:59.55 Carousel.configure :default @18:21:59.66 Carousel.hold @18:22:00.05 Carousel.configure :default @18:22:00.24 Abs.configure :default @18:22:00.44 PhaseSeparator.configure :default @18:22:00.63 Archive.configure :default @18:22:00.73 Archive.hold @18:22:01.34 Archive.configure :default @18:22:01.84 Air.configure :default @18:22:01.93 Air.hold @18:22:02.06 Air.configure :default @18:22:02.22 Lysis1.configure :default @18:22:02.31 Lysis1.hold @18:22:02.45 Lysis1.configure :default @18:22:02.62 Lysis2.configure :default @18:22:02.72 Lysis2.hold @18:22:02.84 Lysis2.configure :default @18:22:03.05 PinPort.configure :default @18:22:03.17 PinPort.hold @18:22:03.21 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @18:22:03.40 PinPort.configure :default @18:22:03.51 All elf controllers running firmware version 4.92 @18:22:03.58 AnalyticModule::Unknown in nadia -- SPR @18:22:04.41 Toroid.seek :clear @18:22:05.93 Carousel.in :default @18:22:06.09 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:22:12.13 Session Begun @18:22:22.68 -> TV.cartridge;delay 2;TV.bypass @18:22:22.90 Toroid.in :hold @18:22:23.03 Toroid.seek :cartridge @18:22:24.93 Toroid.in :default @18:22:25.05 Toroid.seek :clear @18:22:26.91 delay 2 seconds @18:22:29.04 Toroid.in :hold @18:22:29.16 Toroid.seek :bypass @18:22:30.87 Toroid.in :default @18:22:30.98 Toroid.seek :clear Toroid Valve (TV) clear @18:22:48.90 -> SC.to 59 @18:22:48.98 Carousel.seek 59 Storage Carousel (SC) at 59 @18:22:53.39 -> TV.cartridge;delay 2;TV.bypass @18:22:53.59 Toroid.in :hold @18:22:53.75 Toroid.seek :cartridge @18:22:55.66 Toroid.in :default @18:22:55.79 Toroid.seek :clear @18:22:57.64 delay 2 seconds @18:22:59.76 Toroid.in :hold @18:22:59.88 Toroid.seek :bypass @18:23:01.58 Toroid.in :default @18:23:01.69 Toroid.seek :clear Toroid Valve (TV) clear @18:25:55.56 -> Handoff.waste @18:25:55.61 Handoff.select :waste Handoff Valve::Manifold selects waste @18:26:04.86 -> Handoff.blocked @18:26:04.89 Handoff.select :blocked Handoff Valve::Manifold selects blocked @18:26:22.77 -> Handoff.SPR @18:26:22.80 Handoff.select :SPR Handoff Valve::Manifold selects Analytical Module @18:26:32.04 -> Handoff.blocked @18:26:32.07 Handoff.select :blocked Handoff Valve::Manifold selects blocked @18:26:37.81 -> Handoff.waste @18:26:37.85 Handoff.select :waste Handoff Valve::Manifold selects waste @18:26:49.65 -> Syringe.psi 0.682490462942127 @18:27:01.71 -> Handoff.blocked @18:27:01.73 Handoff.select :blocked Handoff Valve::Manifold selects blocked @18:27:28.28 -> PhytoSampler 50,100 @18:27:28.45 NoMethodError in nadia -- undefined method `PhytoSampler' for CmdServer::Client:Class @18:27:37.96 -> PhytoSampler.prime 50,100 @18:27:38.11 Priming sample loop w/50ml, bypass w/100ml @18:27:38.15 SamplePump.setPosition! 0ml @18:27:38.29 Exhaust.close @18:27:38.44 Bypass.open @18:27:40.01 Intake.open @18:27:40.89 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:27:43.53 Vacuum tare is 52% of full scale at 21C (drifted 1%) @18:27:43.76 Abs.in :default @18:27:43.87 Abs.hold @18:27:43.99 PhaseSeparator.in :full @18:27:44.12 PhaseSeparator.hold @18:27:44.41 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.05psi @18:27:50.41 Intake.close @18:27:50.46 Bypass.close @18:27:51.42 Abs.stop @18:27:51.54 PhaseSeparator.stop @18:27:51.67 UserAbort in nadia -- User Abort!! @18:27:59.59 -> PhytoSampler.source=nil @18:28:04.03 -> PhytoSampler.prime 50,100 @18:28:04.17 Priming sample loop w/50ml, bypass w/100ml @18:28:04.19 SamplePump.setPosition! 0ml @18:28:04.33 Bypass.open @18:28:05.87 Intake.open @18:28:06.66 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:28:09.28 Vacuum tare is 52% of full scale at 21C (drifted 1%) @18:28:09.52 Abs.hold @18:28:09.85 SamplePump.in :start @18:28:09.98 SamplePump.seek 100ml,7:10 @18:28:10.54 SamplePump.configure :prime @18:28:57.02 -> Sampler.psi 3.83672186413585 @18:29:09.14 -> Sampler.psi 2.32801888409517 @18:29:15.91 -> Sampler.psi 3.63953576036155 @18:29:17.98 -> Sampler.psi 3.77710746066921 @18:29:27.58 -> Sampler.psi 3.60284997361285 @18:29:47.41 -> Sampler.psi 3.71290733385896 @18:29:49.56 -> SP Sample Pump (SP) at 81.41ml @18:30:09.09 Session Begun @18:30:12.42 Abs.stop @18:30:12.56 SamplePump.setPosition! 0ml @18:30:12.69 Exhaust.open @18:30:14.16 Bypass.close @18:30:15.81 Abs.in :default @18:30:15.96 Abs.hold @18:30:16.36 SamplePump.in :start @18:30:16.51 SamplePump.seek 50ml,3:50 @18:30:17.05 SamplePump.configure :prime @18:31:04.23 -> Sampler.psi 34.5977540529287 @18:31:07.12 -> Abs Absolute Pressure (Leak) Sensor @42psia @18:31:17.95 Abs.stop PhytoSampler @18:31:24.66 -> SP Sample Pump (SP) at 50ml @18:31:34.72 -> Intake.close @18:31:34.76 Intake.close Intake is closed @18:31:52.88 -> Exhaust.close @18:31:52.92 Exhaust.close Exhaust is closed @18:34:28.17 -> ESP.shutdown @18:34:28.44 Gate.power :analytic1,:OFF @18:34:28.52 Gate.power :motor,:OFF @18:34:28.56 Gate.power :core,:OFF @18:34:28.64 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @18:35:19.33 -> ESP.ready! @18:35:19.48 Gate.power :sampler,:OFF,limit to 3500mA @18:35:19.51 Gate.power :skimmer,:OFF,limit to 3500mA @18:35:19.63 Gate.power :main,:ON,limit to 5000mA @18:35:19.67 Gate.power :analytic1,:ON,limit to 4000mA @18:35:19.87 Powering core bus @18:35:22.17 SPR Module 1, FW Rev. B, FPGA Rev. C @18:35:22.66 Plunge.configure :default @18:35:22.83 SamplePump.configure :default @18:35:22.99 Toroid.configure :default @18:35:23.18 Carousel.configure :default @18:35:23.34 Abs.configure :default @18:35:23.54 PhaseSeparator.configure :full @18:35:23.69 Archive.configure :default @18:35:23.85 Air.configure :default @18:35:23.99 Lysis1.configure :default @18:35:24.13 Lysis2.configure :default @18:35:24.29 PinPort.configure :default @18:35:24.44 All elf controllers running firmware version 4.92 @18:35:24.48 Gate.power :motor,:ON,limit to 5000mA @18:35:24.86 Plunge.home.jog 0 @18:35:25.65 Lysis2.home.jog 0 @18:35:25.74 Lysis1.home.jog 0 @18:35:25.82 Air.home.jog 0 @18:35:25.90 Archive.home.jog 0 @18:35:25.96 Toroid.home.jog 0 @18:35:26.08 Carousel.home.jog 0 @18:35:26.21 PinPort.home.jog 0 @18:35:27.30 Plunge.in :homing @18:35:27.54 Plunge.jog 159 @18:35:27.83 Lysis1.in :homing @18:35:27.94 PinPort.in :homing @18:35:28.06 Lysis2.in :homing @18:35:28.11 Toroid.in :homing @18:35:28.22 Air.in :homing @18:35:28.29 Archive.in :homing @18:35:28.71 PinPort.jog -110 @18:35:28.75 Lysis1.jog 1399 @18:35:28.88 Lysis2.jog 1400 @18:35:28.93 Air.jog 1372 @18:35:29.01 Toroid.jog 623 @18:35:29.15 Archive.jog 1396 @18:35:30.34 PinPort.setRawPosition! 0 @18:35:30.36 Plunge.setRawPosition! 50 @18:35:30.62 PinPort.seek :home @18:35:30.64 Plunge.seek :home @18:35:31.01 PinPort.in :default @18:35:31.26 Lysis1.setRawPosition! 50 @18:35:31.30 Plunge.in :default @18:35:31.42 Lysis2.setRawPosition! 50 @18:35:31.54 Air.setRawPosition! 50 @18:35:31.82 Lysis1.seek :home @18:35:32.02 Lysis2.seek :home @18:35:32.08 Air.seek :home @18:35:32.44 Archive.setRawPosition! 50 @18:35:32.48 Toroid.setRawPosition! 0 @18:35:32.58 Lysis1.in :default @18:35:32.86 Archive.seek :home @18:35:32.93 Toroid.seek :home @18:35:33.31 Lysis2.in :default @18:35:33.39 Air.in :default @18:35:33.57 Toroid.in :default @18:35:33.83 Archive.in :default @18:35:34.26 Toroid.seek :clear @18:35:35.99 Carousel.in :default @18:35:36.19 Carousel.home.seek 59 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 59 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:35:49.00 -> SPR.start @18:35:50.28 SPR.command "read_sequences" @18:35:50.43 SPR.command "stats_on" # @18:35:51.38 SPR.start /var/log/esp/spr24aug1418h.spr.bz2 @18:36:05.35 -> SPR.command "start 0 1" @18:36:05.41 SPR.command "start 0 1" @18:36:30.87 -> SPR.command "start 2 14" @18:36:30.89 SPR.command "start 2 14" @18:36:47.59 -> SPR.command "start 3 0" @18:36:47.62 SPR.command "start 3 0" @18:37:04.70 -> SPR.command "start 2 14" @18:37:04.74 SPR.command "start 2 14" @18:37:24.72 -> SPR.stop @18:37:25.26 Ended log spr24aug1418h.spr.bz2 @18:37:25.29 SPR.command "stats_off" @18:43:21.49 -> exit @18:43:21.52 Session Ended @18:43:35.53 -> MainThread.exit # @18:43:35.79 ThreadError in preston -- killed thread @18:43:35.91 Session Ended @18:43:35.99 Session Ended @18:43:36.06 Session Ended @18:45:31.12UTC14-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @18:45:32.84 >BREAK< ESP3 Gateway v1.43 for core bus @18:45:32.86 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:45:36.34 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @18:45:37.03 Sea pressure (Abs) tare is 6% of full scale @18:45:50.67 Polling Can every 2:00 @18:45:50.72 Waiting for espclients... @18:45:50.92 Session Begun @18:45:54.43 Session Begun @18:46:01.88 Session Begun @18:46:02.30 -> PhytoSampler.source=nil @18:46:12.75 -> slots {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [34,32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, 54..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @18:46:26.78 -> fire lysis # @18:46:27.24 Gate.power :sampler,:OFF,limit to 3500mA @18:46:27.27 Gate.power :skimmer,:OFF,limit to 3500mA @18:46:27.34 Gate.power :analytic1,:ON,limit to 4000mA @18:46:28.02 SPR Module 1, FW Rev. B, FPGA Rev. C @18:46:28.28 Plunge.configure :default @18:46:28.48 SamplePump.configure :default @18:46:28.70 Toroid.configure :default @18:46:28.82 Toroid.hold @18:46:28.96 Toroid.configure :default @18:46:29.17 Carousel.configure :default @18:46:29.28 Carousel.hold @18:46:29.47 Carousel.configure :default @18:46:29.69 Abs.configure :default @18:46:30.39 PhaseSeparator.configure :default @18:46:30.61 Archive.configure :default @18:46:30.83 Air.configure :default @18:46:31.01 Lysis1.configure :default @18:46:31.20 Lysis2.configure :default @18:46:31.44 PinPort.configure :default @18:46:31.57 All elf controllers running firmware version 4.92 @18:46:32.43 Toroid.seek :clear @18:46:32.69 Carousel.in :default @18:46:32.88 Carousel.home.seek 59 @18:46:33.21 Selecting Cartridge 34 @18:46:33.60 Carousel.seek 34 @18:46:39.06 Lysis1.in :fast @18:46:39.18 Lysis1.seek :locked @18:46:41.87 Plunge.seek :locked @18:46:42.09 Air.in :fast @18:46:42.20 Air.seek :locked @18:46:43.23 Carousel.reconfigure :locked @18:46:43.48 Powering cartridge bus @18:46:46.15 Gate.setIR 1750,5000,0,6 @18:46:46.28 Gate.setIR 0,5000,0,6 @18:46:46.40 PuckHeater.configure :default @18:46:46.54 Gate.power :heat,:ON,limit to 5000mA @18:46:47.28 Mounted lysis Cartridge board#Q05052 @18:46:47.42 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:46:47.55 GoodNews.email "PhytoSampler filtering up to 1000 ml",Subject: "Sampling microSPR_phyto cartridge in slot 34" @18:46:47.98 Preparing to filter cartridge 34 @18:46:48.37 Air.in :default @18:46:48.57 Air.seek :sealed @18:46:48.76 Priming sample loop w/150ml, bypass w/50ml @18:46:48.78 SamplePump.setPosition! 0ml @18:46:48.94 Exhaust.close @18:46:49.11 Bypass.open @18:46:51.21 Intake.open @18:46:52.13 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:46:54.76 Vacuum tare is 52% of full scale at 21C (drifted 1%) @18:46:54.99 Abs.in :default @18:46:55.11 Abs.hold @18:46:55.46 SamplePump.in :start @18:46:55.61 SamplePump.seek 50ml,3:50 @18:46:56.16 SamplePump.configure :prime @18:46:57.42 upsync @18:47:57.01 Abs.stop @18:47:57.14 SamplePump.setPosition! 0ml @18:47:57.24 Exhaust.open @18:47:58.77 Bypass.close @18:48:00.38 Abs.in :default @18:48:00.51 Abs.hold @18:48:00.90 SamplePump.in :start @18:48:01.03 SamplePump.seek 150ml,10:30 @18:48:01.59 SamplePump.configure :prime @18:51:04.37 Abs.stop @18:51:04.51 Bypass.open @18:51:05.26 Intake.close @18:51:06.14 Toroid.in :hold @18:51:06.27 Toroid.seek :crack @18:51:07.88 Toroid.seek :cartridge @18:51:09.06 Toroid.in :default @18:51:09.19 Toroid.seek :clear @18:51:11.28 Pressurizing cartridge to 26psi above 14.7psia @18:51:11.31 SamplePump.setPosition! 0ml @18:51:11.51 Intake.open @18:51:12.27 Bypass.close @18:51:13.00 Exhaust.close @18:51:13.86 Abs.in :default @18:51:13.98 Abs.hold @18:51:14.27 SamplePump.in :charge @18:51:14.41 SamplePump.seek 2.3ml,42 seconds @18:51:21.29 -> exit @18:51:21.38 Session Ended @18:51:30.63 Abs.stop @18:51:30.74 Intake.close @18:51:31.56 Pressurized cartridge with 1.181ml to 26.5psi @18:51:31.58 Isolated @36.8psia (22.1psi) @18:51:31.63 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @18:51:31.71 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:51:36.32 Cartridge pressure fell 10% to 33.0psia @18:51:36.46 Sampling 1000.0ml @18:51:36.48 SamplePump.setPosition! 0ml @18:51:36.61 Exhaust.open @18:51:37.33 Intake.open @18:51:38.28 Abs.in :default @18:51:38.39 Abs.hold @18:51:38.77 SamplePump.in :start @18:51:38.90 SamplePump.seek 1000ml,1:23:50 @18:51:39.46 SamplePump.configure :sample @18:51:41.87 Cartridge 34 filtering 2/1000ml at 0.55ml/s with 23.4psi vacuum @18:52:41.94 Cartridge 34 filtering 43/1000ml at 0.68ml/s with 16.9psi vacuum 6.6m deep (24.4psia) @18:53:41.92 Cartridge 34 filtering 82/1000ml at 0.65ml/s with 16.5psi vacuum 6.0m deep (23.5psia) @18:54:41.92 Cartridge 34 filtering 119/1000ml at 0.62ml/s with 20.5psi vacuum @18:55:41.92 Cartridge 34 filtering 154/1000ml at 0.59ml/s with 17.9psi vacuum @18:56:41.92 Cartridge 34 filtering 188/1000ml at 0.57ml/s with 25.1psi vacuum @18:57:41.93 Cartridge 34 filtering 221/1000ml at 0.54ml/s with 22.4psi vacuum @18:58:41.92 Cartridge 34 filtering 252/1000ml at 0.52ml/s with 24.0psi vacuum @18:59:41.92 Cartridge 34 filtering 282/1000ml at 0.49ml/s with 23.5psi vacuum @19:00:41.93 Cartridge 34 filtering 310/1000ml at 0.47ml/s with 20.8psi vacuum @19:01:41.91 Cartridge 34 filtering 336/1000ml at 0.43ml/s with 28.6psi vacuum @19:02:41.92 Cartridge 34 filtering 360/1000ml at 0.40ml/s with 22.1psi vacuum @19:03:41.92 Cartridge 34 filtering 382/1000ml at 0.36ml/s with 27.5psi vacuum @19:04:41.92 Cartridge 34 filtering 401/1000ml at 0.33ml/s with 21.4psi vacuum @19:05:41.92 Cartridge 34 filtering 419/1000ml at 0.30ml/s with 22.3psi vacuum @19:06:41.92 Cartridge 34 filtering 435/1000ml at 0.26ml/s with 26.4psi vacuum @19:07:41.93 Cartridge 34 filtering 457/1000ml at 0.36ml/s with 21.6psi vacuum @19:08:41.93 Cartridge 34 filtering 487/1000ml at 0.51ml/s with 22.9psi vacuum @19:08:42.00 Session Ended @19:09:41.92 Cartridge 34 filtering 491/1000ml at 0.07ml/s with 23.8psi vacuum @19:09:42.00 SamplePump.stop @19:09:42.10 Intake.close @19:09:42.18 Exhaust.close @19:09:42.49 Toroid.in :hold @19:09:42.58 Toroid.seek :bypass @19:09:44.26 Toroid.in :default @19:09:44.81 Toroid.seek :clear @19:09:46.56 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @19:09:46.59 Abs.stop @19:09:46.88 Sampled 491.2ml @19:09:47.41 GoodNews.email "After filtering 491.2ml",Subject: "Processing microSPR_phyto cartridge in slot 34" @19:09:48.99 SPR.command "read_sequences" @19:09:49.66 SPR.command "stats_on" @19:09:49.85 SPR.command "start 0 1" @19:09:50.55 SPR.start /var/log/esp/spr24aug1419h.spr.bz2 @19:09:53.01 Handoff.select :waste @19:09:53.13 upsync @19:09:53.23 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:09:59.88 Syringe tare is 51% of full scale at 22C (drifted 0%) @19:09:59.92 PinPort.in :locking @19:10:00.02 PinPort.seek :locked @19:10:00.55 Archive.in :fast @19:10:00.65 Lysis2.in :fast @19:10:00.75 Lysis1.seek 1350uL @19:10:00.79 Archive.seek :full @19:10:00.97 Lysis2.seek 950uL @19:10:05.81 Plunge.seek :reagent @19:10:06.76 Evacuating puck with filter bubble point of 24psi @19:10:06.80 PinPort.seek :locked @19:10:06.94 Handoff.select :waste @19:10:06.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:10:13.59 Air syringe pumping up to 1 strokes to reach 8psi @19:10:13.61 Air stroke #1 of 1 @19:10:13.75 Air.in :pressurize @19:10:13.84 Air.seek :pressurized @19:10:19.56 1.7psi after pressurize @19:10:19.59 Air.in :compress @19:10:19.73 Air.seek :evac @19:10:22.83 4.0psi after compress @19:10:39.45 Session Begun @19:10:39.72 0.6psi after compress (stable) @19:10:40.35 Air.in :default @19:10:40.51 Air.hold @19:10:40.88 at 0.4psi Opened to waste @19:10:40.90 Handoff.select :blocked @19:10:41.13 Archive syringe pumping up to 8 strokes to reach 24psi @19:10:41.15 Archive stroke #1 of 8 @19:10:41.42 Archive.in :pressurize @19:10:41.58 Archive.seek :pressurized @19:10:54.06 2.3psi after pressurize @19:10:59.66 2.2psi after pressurize (stable) @19:10:59.69 Archive.in :compress @19:10:59.83 Archive.seek :empty @19:11:15.58 19.0psi after compress @19:11:28.69 20.6psi after compress (stable) @19:11:28.73 Archive.in :gulp @19:11:28.86 Archive.seek :topVent @19:11:35.25 21.5psi after gulp @19:11:40.88 21.0psi after gulp (stable) @19:11:40.89 Archive stroke #2 of 8 @19:11:41.11 Archive.in :pressurize @19:11:41.22 Archive.seek :pressurized @19:11:53.69 21.7psi after pressurize @19:11:59.29 20.9psi after pressurize (stable) @19:11:59.35 Archive.in :compress @19:11:59.50 Archive.seek :empty @19:12:04.27 -> showlog -200 @19:12:07.95 Reached 23.6 of 24.0psi after pumping 3286uL (1.90 strokes) @19:12:08.00 Archive.in :default @19:12:08.21 Archive.hold @19:12:08.54 Handoff.select :waste @19:12:08.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:12:21.88 delay 1:00 @19:13:21.91 Handoff.select :blocked @19:13:22.12 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:13:44.75 Handoff.select :waste @19:13:44.95 1.3psi at evacEnd @19:13:44.97 Handoff.select :blocked @19:13:45.17 Archive.in :fast @19:13:45.26 Archive.seek :topVent @19:13:51.87 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @19:13:58.46 Stable @1.3psi after Handoff blocked @19:13:58.50 Archive syringe pumping up to 8 strokes to reach 25psi @19:13:58.57 Archive stroke #1 of 8 @19:13:58.74 Archive.in :pressurize @19:13:58.84 Archive.seek :pressurized @19:14:11.39 2.1psi after pressurize @19:14:11.42 Archive.in :compress @19:14:11.57 Archive.seek :empty @19:14:27.42 16.3psi after compress @19:14:38.02 16.1psi after compress (stable) @19:14:38.05 Archive.in :gulp @19:14:38.20 Archive.seek :topVent @19:14:44.55 17.1psi after gulp @19:14:50.15 16.5psi after gulp (stable) @19:14:50.17 Archive stroke #2 of 8 @19:14:50.33 Archive.in :pressurize @19:14:50.43 Archive.seek :pressurized @19:15:02.90 17.0psi after pressurize @19:15:02.94 Archive.in :compress @19:15:03.08 Archive.seek :empty @19:15:14.41 Reached 25.4 of 25.3psi after pumping 3365uL (1.95 strokes) @19:15:14.44 Archive.in :default @19:15:14.60 Archive.hold @19:15:14.73 Handoff.select :waste @19:15:14.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:15:29.02 delay 1:00 @19:16:29.04 Handoff.select :blocked @19:16:29.23 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:16:51.83 Handoff.select :waste @19:16:52.09 0.8psi at evacEnd @19:16:52.13 Air.seek :fullVent @19:16:53.52 Archive.in :fast @19:16:53.62 Archive.seek :topVent @19:17:00.49 Seawater evacuated, Pressure above/below filter released. @19:17:00.66 Lysis1.in :slow @19:17:00.77 Lysis1.seek 1050uL @19:17:30.42 delay 5 seconds @19:17:35.45 Handoff.select :blocked @19:17:35.75 Lysis1.seek 450uL @19:18:34.88 delay 3 seconds @19:18:38.01 Air.in mix with 1.57127 parts Lysis1 @19:18:38.78 Air.seek :evac @19:18:38.97 Lysis1.seek 300uL @19:18:53.90 Lysis1.seek :empty @19:19:23.72 Lysis buffer delivered @28.1psi @19:19:23.75 Polling Can every 2:00 @19:19:24.34 PuckHeater.configure :default #ambient 21.6C @19:19:24.43 PuckHeater.seek 79.8C @19:20:23.20 PuckHeater.hold 2:00,79.8C @19:22:23.03 Polling Can every 2:00 @19:22:23.60 PuckHeater.configure :default #ambient 21.6C @19:22:23.71 PuckHeater.hold 30 seconds,78.7C @19:22:53.86 Polling Can every 2:00 @19:22:54.41 PuckHeater.configure :default #ambient 21.6C @19:22:54.52 PuckHeater.hold 30 seconds,78.2C @19:23:24.66 Polling Can every 2:00 @19:23:25.21 PuckHeater.configure :default #ambient 21.5C @19:23:25.31 PuckHeater.hold 10:00,77.7C @19:33:23.80 Lysis complete. Syringe pressure @33.9psi @19:33:23.86 Archive syringe pumping up to 5 strokes to reach 40psi @19:33:23.89 Archive stroke #1 of 5 @19:33:24.03 Archive.in :pressurize @19:33:24.14 Archive.seek :pressurized @19:33:36.63 33.1psi after pressurize @19:33:42.22 32.1psi after pressurize (stable) @19:33:42.26 Archive.in :compress @19:33:42.39 Archive.seek :empty @19:33:50.85 Reached 42.0 of 40.0psi after pumping 1561uL (0.90 strokes) @19:33:50.87 Archive.in :default @19:33:51.05 Archive.hold @19:33:51.25 delay 2 seconds @19:33:53.28 Archive.in :fast @19:33:53.43 Archive.seek :full @19:34:00.19 Lysis1.seek 138uL @19:34:13.93 delay 1 second @19:34:14.97 Archive.seek :full @19:34:15.09 Lysis1.seek :empty @19:34:28.88 Handoff.select :blocked @19:34:29.04 Initial air pressure @47.9psi @19:34:29.15 Lysis1.in :extract @19:34:29.28 Lysis1.seek 600uL,nil @19:34:31.95 Archive syringe pumping up to 6 strokes to reach 25psi @19:34:31.97 Archive stroke #1 of 6 @19:34:32.13 Archive.in :pressurize @19:34:32.25 Archive.seek :pressurized @19:34:44.79 11.5psi after pressurize @19:34:50.93 10.9psi after pressurize (stable) @19:34:50.97 Archive.in :compress @19:34:51.10 Archive.seek :empty @19:34:57.53 Archive.in :default @19:34:57.60 Archive.hold @19:34:57.88 Initial 600uL lysate @16.0psi @19:34:57.90 Handoff.select :blocked @19:34:58.09 Initial air pressure @16.6psi @19:34:58.22 Lysis1.seek 780uL,nil @19:34:59.60 Archive syringe pumping up to 6 strokes to reach 17psi @19:34:59.61 Archive stroke #1 of 6 @19:34:59.80 Archive.in :compress @19:34:59.93 Archive.seek :empty @19:35:00.76 Archive.in :default @19:35:00.82 Archive.hold @19:35:01.16 Recovered 780uL lysate @15.1psi @19:35:01.18 Air.in :halfSpeed @19:35:01.32 Air.seek :fullVent @19:35:03.49 Puck Top Vented, bottom @16.9psi @19:35:03.52 Handoff.select :waste @19:35:03.71 delay 5 seconds @19:35:08.80 Handoff open to waste, bottom @0.4psi @19:35:08.83 Air.in :default @19:35:08.96 Air.seek :evac @19:35:10.31 Lysis2.in :DAmix @19:35:10.51 Lysis1.in :DAmix @19:35:11.29 Lysis2.seek 800uL @19:35:11.43 Lysis1.seek 630uL @19:35:32.28 delay 5 seconds @19:35:37.31 Handoff.select SPR @19:35:37.54 SPR.command "start 2 14" @19:35:38.95 SPR stabilized @19:35:39.17 delay 10 seconds @19:35:49.19 SPR.command "start 2 0" @19:35:52.32 Waiting up to 11:00 for SPR to enter state 1 @19:37:49.97 SPR.command "hold_on" @19:37:51.42 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @19:37:52.18 Lysis2.seek 350uL @19:37:52.24 Lysis1.seek 180uL @19:38:54.45 delay 15 seconds @19:39:09.48 SPR.command "hold_off" @19:39:12.83 Waiting up to 1:00:00 for SPR to enter state 7 @19:53:31.63

Session Begun @19:54:36.44 -> #Delivery to the SPR was bubble free watched by Paul & Reagan @19:54:38.14 -> exit @19:54:38.16 Session Ended @19:55:28.39 SPRsummary:-3.64RIU,1.33e+09RIU,14.6RIU,-4.61RIU,none,high,high after 2991s @20:09:45.28 SPR analysis completed @20:09:53.89 Ended log spr24aug1419h.spr.bz2 @20:09:53.91 SPR.command "stats_off" @20:09:54.42 Handoff.select :close @20:09:54.62 Gate.power :heat,:OFF @20:09:54.64 Gate.power :cartridge,:OFF @20:09:54.79 PinPort.in :unlocking @20:09:54.88 PinPort.seek :clear @20:09:55.41 Carousel.in :default @20:09:55.57 Carousel.home.seek 34 @20:09:55.94 Plunge.seek :locked @20:09:56.87 Archive.in :unlocking @20:09:57.05 Archive.seek :clear @20:09:57.11 Air.in :unlocking @20:09:57.42 Air.seek :clear @20:09:57.48 Lysis1.in :unlocking @20:09:57.71 Lysis1.seek :clear @20:09:58.35 Lysis2.in :unlocking @20:09:58.43 Lysis2.seek :clear @20:10:02.40 Gate.power :analytic1,:OFF @20:10:02.43 Gate.power :motor,:OFF @20:10:02.48 Gate.power :core,:OFF @20:10:02.51 Gate.power :main,:OFF @20:10:02.55 Safely stopped and ready to power off @20:12:28.28 Session Ended @20:16:12.98 Session Ended @20:54:49.83 Session Begun @20:55:03.23 -> showlog -50 @20:55:27.08 -> exit @20:55:27.11 Session Ended @21:46:08.11 Session Begun @21:46:36.49 -> PhytoSampler.prime 100, 500 @21:46:36.55 BusOff in bill -- core bus was not powered while reading Storage Carousel (SC)'s status @21:46:43.97 -> ESP.ready! @21:46:44.02 Gate.power :sampler,:OFF,limit to 3500mA @21:46:44.04 Gate.power :skimmer,:OFF,limit to 3500mA @21:46:44.08 Gate.power :main,:ON,limit to 5000mA @21:46:44.12 Gate.power :analytic1,:ON,limit to 4000mA @21:46:44.23 Powering core bus @21:46:46.57 SPR Module 1, FW Rev. B, FPGA Rev. C @21:46:47.01 Plunge.configure :default @21:46:47.12 SamplePump.configure :default @21:46:47.23 Toroid.configure :default @21:46:47.33 Carousel.configure :default @21:46:47.48 Abs.configure :default @21:46:47.59 PhaseSeparator.configure :default @21:46:47.70 Archive.configure :default @21:46:47.80 Air.configure :default @21:46:47.89 Lysis1.configure :default @21:46:47.97 Lysis2.configure :default @21:46:48.07 PinPort.configure :default @21:46:48.14 All elf controllers running firmware version 4.92 @21:46:48.17 Gate.power :motor,:ON,limit to 5000mA @21:46:48.59 Plunge.home.jog 0 @21:46:49.21 PinPort.home.jog 0 @21:46:49.25 Lysis1.home.jog 0 @21:46:49.29 Archive.home.jog 0 @21:46:49.33 Carousel.home.jog 0 @21:46:49.40 Toroid.home.jog 0 @21:46:49.46 Lysis2.home.jog 0 @21:46:49.51 Air.home.jog 0 @21:46:50.33 Plunge.in :homing @21:46:50.62 Plunge.jog 1607 @21:46:50.87 PinPort.in :homing @21:46:50.91 Lysis2.in :homing @21:46:50.98 Air.in :homing @21:46:51.02 Toroid.in :homing @21:46:51.06 Archive.in :homing @21:46:51.14 Lysis1.in :homing @21:46:51.32 PinPort.jog -111 @21:46:51.47 Lysis2.jog 1399 @21:46:51.53 Air.jog 1381 @21:46:51.58 Toroid.jog 623 @21:46:51.70 Lysis1.jog 150 @21:46:51.73 Archive.jog 1397 @21:46:52.21 PinPort.setRawPosition! 0 @21:46:52.23 Plunge.setRawPosition! 50 @21:46:52.34 PinPort.seek :home @21:46:52.45 Plunge.seek :home @21:46:52.64 PinPort.in :default @21:46:52.66 Lysis1.setRawPosition! 50 @21:46:52.82 Lysis1.seek :home @21:46:52.96 Plunge.in :default @21:46:53.07 Lysis2.setRawPosition! 50 @21:46:53.64 Lysis2.seek :home @21:46:53.89 Air.setRawPosition! 50 @21:46:53.91 Archive.setRawPosition! 50 @21:46:53.95 Lysis1.in :default @21:46:54.15 Archive.seek :home @21:46:54.21 Air.seek :home @21:46:54.51 Toroid.setRawPosition! 0 @21:46:54.53 Lysis2.in :default @21:46:54.69 Toroid.seek :home @21:46:54.84 Archive.in :default @21:46:54.89 Toroid.in :default @21:46:54.95 Air.in :default @21:46:55.37 Toroid.seek :clear @21:46:57.14 Carousel.in :default @21:46:57.29 Carousel.home.seek 34 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 34 Abs = Absolute Pressure (Leak) Sensor @26psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @21:47:04.65 -> PhytoSampler.prime 100, 500 @21:47:04.75 Priming sample loop w/100ml, bypass w/500ml @21:47:04.78 SamplePump.setPosition! 0ml @21:47:04.87 Bypass.open @21:47:06.37 Intake.open @21:47:07.23 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:47:09.86 Vacuum tare is 53% of full scale at 20C (drifted 1%) @21:47:10.02 Abs.in :default @21:47:10.10 Abs.hold @21:47:10.37 SamplePump.in :start @21:47:10.46 SamplePump.seek 500ml,33:50 @21:47:11.07 SamplePump.configure :prime @21:57:20.69 Abs.stop @21:57:20.77 SamplePump.setPosition! 0ml @21:57:20.85 Exhaust.open @21:57:22.23 Bypass.close @21:57:23.79 Abs.in :default @21:57:23.88 Abs.hold @21:57:24.20 SamplePump.in :start @21:57:24.29 SamplePump.seek 100ml,7:10 @21:57:24.89 SamplePump.configure :prime @21:59:26.61 Abs.stop PhytoSampler @22:02:16.60 -> PhytoSampler.prime 150, 10 @22:02:16.70 Priming sample loop w/150ml, bypass w/10ml @22:02:16.72 SamplePump.setPosition! 0ml @22:02:16.80 Exhaust.close @22:02:17.48 Bypass.open @22:02:19.66 Abs.in :default @22:02:19.75 Abs.hold @22:02:20.05 SamplePump.in :start @22:02:20.14 SamplePump.seek 10ml,1:10 @22:02:20.73 SamplePump.configure :prime @22:02:32.70 Abs.stop @22:02:32.78 SamplePump.setPosition! 0ml @22:02:32.87 Exhaust.open @22:02:34.27 Bypass.close @22:02:35.83 Abs.in :default @22:02:35.91 Abs.hold @22:02:36.24 SamplePump.in :start @22:02:36.34 SamplePump.seek 150ml,10:30 @22:02:36.93 SamplePump.configure :prime @22:05:39.64 Abs.stop PhytoSampler @22:05:46.91 -> SPR.start @22:05:48.09 SPR.command "read_sequences" @22:05:48.16 SPR.command "stats_on" # @22:05:49.04 SPR.start /var/log/esp/spr24aug1422h.spr.bz2 @22:06:01.44 -> SPR.command "start 3 0" @22:06:01.47 SPR.command "start 3 0" @22:52:52.22 -> SPR.command "start 2 14" @22:52:52.26 SPR.command "start 2 14" @22:52:57.25 -> SPR.stop @22:53:00.48 Ended log spr24aug1422h.spr.bz2 @22:53:00.50 SPR.command "stats_off" @23:03:11.55 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: spr24aug1419h.spr.bz2}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, 54..52 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @23:04:19.58 -> Cartridge.release 54 1 @23:04:29.87 -> runStandard # @23:04:30.83 Toroid.seek :clear @23:04:31.48 Carousel.in :default @23:04:31.70 Carousel.home.seek 34 @23:04:31.99 Selecting Cartridge 54 @23:04:32.32 Carousel.seek 54 @23:04:36.75 Lysis1.in :fast @23:04:36.83 Lysis1.seek :locked @23:04:39.51 Plunge.seek :locked @23:04:39.71 Air.in :fast @23:04:39.79 Air.seek :locked @23:04:40.78 Carousel.reconfigure :locked @23:04:40.96 Powering cartridge bus @23:04:43.63 Gate.setIR 1750,5000,0,6 @23:04:43.77 Gate.setIR 0,5000,0,6 @23:04:43.87 PuckHeater.configure :default #ambient 21.5C @23:04:43.96 Gate.power :heat,:ON,limit to 5000mA @23:04:44.58 Mounted lysis Cartridge @23:04:44.64 WARNING: Using default puck temperature sensor calibration @23:04:44.66 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:04:45.01 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 54" @23:04:45.56 SPR.command "stats_on" @23:04:46.56 SPR.command "start 0 1" @23:04:47.26 SPR.start /var/log/esp/spr24aug1423h.spr.bz2 @23:04:49.30 Waiting for baseline SPR stabilization @23:04:49.32 Handoff.select :waste @23:04:49.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:04:50.67 upsync @23:04:56.20 Syringe tare is 51% of full scale at 21C (drifted 0%) @23:04:56.23 PinPort.in :locking @23:04:56.33 PinPort.seek :locked @23:04:56.90 Archive.in :fast @23:04:56.95 Lysis2.in :fast @23:04:57.09 Lysis1.seek 1100uL @23:04:57.25 Archive.seek :full @23:04:57.31 Lysis2.seek 1100uL @23:05:01.73 delayUntil "23:14:48.86" @23:14:48.87 Lysis2.in :DAmix @23:14:49.02 Lysis1.in :DAmix @23:14:49.64 Lysis2.seek 950uL @23:14:49.72 Lysis1.seek 950uL @23:15:10.62 delay 5 seconds @23:15:15.65 Handoff.select SPR @23:15:15.81 SPR.command "start 2 14" @23:15:16.39 SPR stabilized @23:15:16.57 delay 10 seconds @23:15:26.59 SPR.command "start 2 0" @23:15:29.75 Waiting up to 11:00 for SPR to enter state 1 @23:17:27.43 SPR.command "hold_on" @23:17:29.33 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @23:17:29.98 Lysis2.seek 500uL @23:17:30.02 Lysis1.seek 500uL @23:18:32.24 delay 15 seconds @23:18:47.26 SPR.command "hold_off" @23:18:50.27 Waiting up to 1:00:00 for SPR to enter state 7 @23:21:11.91 Session Ended @23:35:04.89 SPRsummary:-18.1RIU,207RIU,245RIU,-12.6RIU after 4911s @23:49:21.94 delay 10 seconds @23:49:31.96 SPR.command "start 2 0" @23:49:34.65 Waiting up to 11:00 for SPR to enter state 1 @23:51:32.32 SPR.command "hold_on" @23:51:33.70 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @23:51:34.36 Lysis2.seek 275uL @23:51:34.42 Lysis1.seek 275uL @23:52:05.57 delay 15 seconds @23:52:20.59 SPR.command "hold_off" @23:52:23.95 Waiting up to 1:00:00 for SPR to enter state 7 @00:22:56.56UTC15-Aug-24 delay 10 seconds @00:23:06.58 SPR.command "start 2 0" @00:23:09.79 Waiting up to 11:00 for SPR to enter state 1 @00:25:07.89 SPR.command "hold_on" @00:25:09.28 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @00:25:09.95 Lysis2.seek 50uL @00:25:10.01 Lysis1.seek 50uL @00:25:41.19 delay 15 seconds @00:25:56.21 SPR.command "hold_off" @00:26:00.04 Waiting up to 1:00:00 for SPR to enter state 7 @00:56:32.95 SPR analysis completed @00:56:48.73 Ended log spr24aug1423h.spr.bz2 @00:56:48.75 SPR.command "stats_off" @15:32:17.51 Session Begun @15:32:22.63 -> showlog -100 @15:32:56.97 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: spr24aug1419h.spr.bz2}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug1423h.spr.bz2}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @17:57:48.45 Session Ended @12:15:24.17UTC19-Aug-24

Environmental Sample Processor built 01-Jul-24 (3743e013) @12:15:27.88 >BREAK< ESP3 Gateway v1.43 for core bus @12:15:27.90 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:15:31.58 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @12:15:32.65 Sea pressure (Abs) tare is 6% of full scale @12:15:47.43 Polling Can every 2:00 @12:15:47.49 Waiting for espclients... @12:15:52.56 Session Begun @12:15:55.58 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @12:16:34.04 -> ESP.ready! @12:16:34.08 Gate.power :sampler,:OFF,limit to 3500mA @12:16:34.11 Gate.power :skimmer,:OFF,limit to 3500mA @12:16:34.14 Gate.power :main,:ON,limit to 5000mA @12:16:34.19 Gate.power :analytic1,:ON,limit to 4000mA @12:16:34.37 Powering core bus @12:16:36.64 SPR Module 1, FW Rev. B, FPGA Rev. C @12:16:37.14 Plunge.configure :default @12:16:37.25 SamplePump.configure :default @12:16:37.35 Toroid.configure :default @12:16:37.45 Carousel.configure :default @12:16:37.55 Abs.configure :default @12:16:37.65 PhaseSeparator.configure :default @12:16:37.75 Archive.configure :default @12:16:37.84 Air.configure :default @12:16:37.92 Lysis1.configure :default @12:16:37.99 Lysis2.configure :default @12:16:38.10 PinPort.configure :default @12:16:38.19 All elf controllers running firmware version 4.92 @12:16:38.22 Gate.power :motor,:ON,limit to 5000mA @12:16:38.55 Plunge.home.jog 0 @12:16:39.77 PinPort.home.jog 0 @12:16:39.82 Lysis1.home.jog 0 @12:16:39.87 Air.home.jog 0 @12:16:39.90 Carousel.home.jog 0 @12:16:39.95 Archive.home.jog 0 @12:16:39.98 Toroid.home.jog 0 @12:16:40.02 Lysis2.home.jog 0 @12:16:40.83 Plunge.in :homing @12:16:41.12 Plunge.jog 1600 @12:16:41.32 Lysis2.in :homing @12:16:41.38 Lysis1.in :homing @12:16:41.43 Archive.in :homing @12:16:41.48 PinPort.in :homing @12:16:41.52 Toroid.in :homing @12:16:41.55 Air.in :homing @12:16:41.83 Lysis1.jog 9593 @12:16:41.90 Archive.jog 1307 @12:16:41.94 Lysis2.jog 9595 @12:16:42.00 PinPort.jog -2350 @12:16:42.05 Air.jog 1156 @12:16:42.09 Toroid.jog 624 @12:16:42.50 Plunge.setRawPosition! 50 @12:16:42.57 Plunge.seek :home @12:16:42.87 Plunge.in :default @12:16:43.32 Archive.setRawPosition! 50 @12:16:43.41 Archive.seek :home @12:16:43.53 Air.setRawPosition! 50 @12:16:43.60 Air.seek :home @12:16:43.72 Archive.in :default @12:16:43.90 Air.in :default @12:16:44.51 Toroid.setRawPosition! 0 @12:16:44.58 Toroid.seek :home @12:16:44.74 Toroid.in :default @12:16:45.58 PinPort.setRawPosition! 0 @12:16:45.66 PinPort.seek :home @12:16:45.78 PinPort.in :default @12:16:47.14 Lysis1.setRawPosition! 50 @12:16:47.23 Lysis1.seek :home @12:16:47.31 Lysis2.setRawPosition! 50 @12:16:47.41 Lysis2.seek :home @12:16:47.56 Lysis1.in :default @12:16:47.71 Lysis2.in :default @12:16:48.06 Toroid.seek :clear @12:16:49.80 Carousel.in :default @12:16:49.94 Carousel.home.seek 54 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @12:17:27.02 -> SPR.start @12:17:28.18 SPR.command "read_sequences" @12:17:28.60 SPR.command "stats_on" # @12:17:29.48 SPR.start /var/log/esp/spr24aug1912h.spr.bz2 @12:17:51.89 -> SPR.command "start 3 0" @12:17:51.91 SPR.command "start 3 0" @13:02:37.32 -> SPR.stop @13:02:39.71 Ended log spr24aug1912h.spr.bz2 @13:02:39.73 SPR.command "stats_off" @13:51:31.29 -> SPR.start @13:51:31.36 SPR.command "stats_on" # @13:51:32.34 SPR.start /var/log/esp/spr24aug1913h.spr.bz2 @13:51:38.59 -> SPR.command "start 3 0" @13:51:38.64 SPR.command "start 3 0" @14:17:59.82 -> SPR.command "start 2 14" @14:17:59.84 SPR.command "start 2 14" @14:18:08.36 -> SPR.stop @14:18:19.91 Ended log spr24aug1913h.spr.bz2 @14:18:19.93 SPR.command "stats_off" @14:30:52.74 -> BACsampler.xray @14:31:51.54 -> BACsampler.sampler.nil @14:31:51.58 NoMethodError in bill -- undefined method `sampler' for BACsampler:Cartridge::Sampler @14:32:05.57 -> BACsampler.source=nil @14:32:11.56 -> BACsampler.xray @14:34:02.48 -> BACsampler.bypassVolume=10 10 @14:34:18.98 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @14:35:46.97 -> Cartridge.reserve [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7] 20 @14:35:54.23 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,5,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @14:36:40.23 -> Cartridge.release 5 1 @14:37:39.20 -> fire archive # @14:37:40.17 Toroid.seek :clear @14:37:40.38 Carousel.in :default @14:37:40.51 Carousel.home.seek 54 @14:37:40.85 Selecting Cartridge 5 @14:37:41.18 Carousel.seek 5 @14:37:51.51 Lysis1.in :fast @14:37:51.60 Lysis1.seek :locked @14:37:54.25 Plunge.seek :locked @14:37:54.45 Air.in :fast @14:37:54.53 Air.seek :locked @14:37:55.52 Carousel.reconfigure :locked @14:37:55.71 Powering cartridge bus @14:37:58.38 Gate.setIR 1750,5000,0,6 @14:37:58.52 Gate.setIR 0,5000,0,6 @14:37:58.62 Gate.power :cartridge,:OFF @14:37:59.21 Mounted archive Cartridge @14:37:59.35 GoodNews.email "BACsampler filtering up to 1000 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 5" @14:37:59.69 Preparing to filter cartridge 5 @14:38:00.05 Priming sample loop w/150ml, bypass w/10ml @14:38:00.06 SamplePump.setPosition! 0ml @14:38:00.15 Bypass.open @14:38:01.67 Intake.open @14:38:02.48 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:38:05.12 Vacuum tare is 52% of full scale at 24C (drifted 1%) @14:38:05.32 Abs.in :default @14:38:05.40 Abs.hold @14:38:05.59 upsync @14:38:05.76 SamplePump.in :start @14:38:05.87 SamplePump.seek 10ml,1:10 @14:38:06.45 SamplePump.configure :prime @14:38:18.44 Abs.stop @14:38:18.53 SamplePump.setPosition! 0ml @14:38:18.61 Exhaust.open @14:38:20.02 Bypass.close @14:38:21.62 Abs.in :default @14:38:21.71 Abs.hold @14:38:22.05 SamplePump.in :start @14:38:22.14 SamplePump.seek 150ml,10:30 @14:38:22.74 SamplePump.configure :prime @14:41:25.58 Abs.stop @14:41:25.69 Bypass.open @14:41:26.42 Intake.close @14:41:27.21 Toroid.in :hold @14:41:27.30 Toroid.seek :crack @14:41:28.87 Toroid.seek :cartridge @14:41:30.01 Toroid.in :default @14:41:30.09 Toroid.seek :clear @14:41:32.17 Pressurizing cartridge to 26psi above 15.7psia @14:41:32.18 SamplePump.setPosition! 0ml @14:41:32.28 Intake.open @14:41:32.94 Bypass.close @14:41:33.64 Exhaust.close @14:41:34.44 Abs.in :default @14:41:34.53 Abs.hold @14:41:34.81 SamplePump.in :charge @14:41:35.44 SamplePump.seek 2.3ml,42 seconds @14:41:48.54 Abs.stop @14:41:48.64 Intake.close @14:41:49.38 Pressurized cartridge with 0.765ml to 26.3psi @14:41:49.39 Isolated @40.7psia (25.0psi) @14:41:49.42 Verifying cartridge holds at least 0.65..0.85 of 40.7psia @14:41:49.48 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:41:54.10 Cartridge pressure fell 1% to 40.3psia @14:41:54.23 Sampling 1000.0ml @14:41:54.24 SamplePump.setPosition! 0ml @14:41:54.33 Exhaust.open @14:41:55.00 Intake.open @14:41:55.89 Abs.in :default @14:41:55.96 Abs.hold @14:41:56.32 SamplePump.in :start @14:41:56.42 SamplePump.seek 1000ml,1:23:50 @14:41:57.01 SamplePump.configure :sample @14:41:59.42 Cartridge 5 filtering 2/1000ml at 0.71ml/s with 22.6psi vacuum @14:42:59.48 Cartridge 5 filtering 41/1000ml at 0.65ml/s with 21.2psi vacuum @14:43:59.49 Cartridge 5 filtering 79/1000ml at 0.63ml/s with 24.5psi vacuum @14:44:59.48 Cartridge 5 filtering 116/1000ml at 0.63ml/s with 26.6psi vacuum @14:45:59.49 Cartridge 5 filtering 153/1000ml at 0.62ml/s with 23.1psi vacuum @14:46:59.48 Cartridge 5 filtering 190/1000ml at 0.61ml/s with 19.5psi vacuum @14:47:59.49 Cartridge 5 filtering 227/1000ml at 0.61ml/s with 25.8psi vacuum @14:48:59.46 Cartridge 5 filtering 263/1000ml at 0.60ml/s with 24.1psi vacuum @14:49:59.46 Cartridge 5 filtering 299/1000ml at 0.60ml/s with 19.4psi vacuum @14:50:59.49 Cartridge 5 filtering 334/1000ml at 0.60ml/s with 23.0psi vacuum @14:51:59.46 Cartridge 5 filtering 370/1000ml at 0.59ml/s with 21.1psi vacuum @14:52:59.46 Cartridge 5 filtering 406/1000ml at 0.59ml/s with 25.8psi vacuum @14:53:59.47 Cartridge 5 filtering 441/1000ml at 0.59ml/s with 25.8psi vacuum @14:54:59.49 Cartridge 5 filtering 477/1000ml at 0.59ml/s with 23.9psi vacuum @14:55:59.48 Cartridge 5 filtering 512/1000ml at 0.59ml/s with 26.6psi vacuum @14:56:59.47 Cartridge 5 filtering 547/1000ml at 0.59ml/s with 25.4psi vacuum @14:57:59.48 Cartridge 5 filtering 582/1000ml at 0.58ml/s with 21.4psi vacuum @14:58:59.46 Cartridge 5 filtering 617/1000ml at 0.58ml/s with 23.1psi vacuum @14:59:59.48 Cartridge 5 filtering 652/1000ml at 0.58ml/s with 21.1psi vacuum @15:00:59.94 Cartridge 5 filtering 687/1000ml at 0.58ml/s with 20.4psi vacuum 3.8m deep (20.4psia) @15:01:59.47 Cartridge 5 filtering 721/1000ml at 0.58ml/s with 24.6psi vacuum @15:02:59.47 Cartridge 5 filtering 756/1000ml at 0.58ml/s with 18.7psi vacuum @15:03:59.47 Cartridge 5 filtering 790/1000ml at 0.57ml/s with 28.0psi vacuum @15:04:59.47 Cartridge 5 filtering 825/1000ml at 0.57ml/s with 22.4psi vacuum 4.2m deep (20.9psia) @15:05:59.48 Cartridge 5 filtering 859/1000ml at 0.57ml/s with 24.3psi vacuum @15:06:59.47 Cartridge 5 filtering 893/1000ml at 0.57ml/s with 26.2psi vacuum @15:07:59.48 Cartridge 5 filtering 927/1000ml at 0.57ml/s with 22.9psi vacuum @15:08:59.48 Cartridge 5 filtering 961/1000ml at 0.57ml/s with 18.7psi vacuum @15:09:59.48 Cartridge 5 filtering 995/1000ml at 0.57ml/s with 23.4psi vacuum 4.6m deep (21.5psia) @15:10:07.79 Abs.stop @15:10:08.04 Intake.close @15:10:08.13 Exhaust.close @15:10:08.38 Toroid.in :hold @15:10:08.46 Toroid.seek :bypass @15:10:10.11 Toroid.in :default @15:10:10.20 Toroid.seek :clear @15:10:11.84 Sampled 1000.0ml @15:10:12.34 GoodNews.email "After filtering 1000.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 5" @15:10:12.87 Plunge.seek :reagent @15:10:13.72 Sample loop closed. Ready to Deliver Reagents @15:10:13.77 Archive.seek :sealed @15:10:17.76 Archive.seek :evac @15:10:17.79 upsync @15:10:32.10 delay 1:00 @15:11:32.14 Archive.seek :clear @15:11:49.88 RNAlater soak for 10:00 @15:21:49.91 Archive.seek :tVent @15:21:52.44 delay 1 second @15:21:53.47 Archive.seek :evac @15:22:09.14 delay 10 seconds @15:22:19.18 Archive.seek :tVent @15:22:34.84 delay 1 second @15:22:35.87 Archive.seek :evac @15:22:51.53 delay 10 seconds @15:23:01.57 Sample preserved and evacuated @15:23:01.88 Carousel.in :default @15:23:02.04 Carousel.home.seek 5 @15:23:02.80 Plunge.seek :locked @15:23:03.82 Archive.in :unlocking @15:23:03.99 Archive.seek :clear @15:23:04.15 Air.in :unlocking @15:23:04.29 Air.seek :clear @15:23:04.36 Lysis1.in :unlocking @15:23:04.61 Lysis1.seek :clear @15:23:08.67 Gate.power :analytic1,:OFF @15:23:08.69 Gate.power :motor,:OFF @15:23:08.76 Gate.power :core,:OFF @15:23:08.80 Gate.power :main,:OFF @15:23:08.83 Safely stopped and ready to power off @15:23:14.02 -> slot {[4,2,1] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, [32,30,28,26,24,22,20,18,16,14,12,10,8,6] => { Type: :microSPR_phyto, State: :dry}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @15:26:03.51 -> Cartridge.release [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] 21 @15:26:55.65 -> Cartridge.release [4, 2, 1] 3 @15:27:02.81 -> slot {[32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @16:51:18.90 -> Cartridge.unlock @16:51:18.98 BusOff in bill -- core bus was not powered while reading Handoff Pin Port (PP)'s status @16:51:24.09 -> ESP.ready! @16:51:24.17 Gate.power :sampler,:OFF,limit to 3500mA @16:51:24.19 Gate.power :skimmer,:OFF,limit to 3500mA @16:51:24.23 Gate.power :main,:ON,limit to 5000mA @16:51:24.27 Gate.power :analytic1,:ON,limit to 4000mA @16:51:24.38 Powering core bus @16:51:26.74 SPR Module 1, FW Rev. B, FPGA Rev. C @16:51:27.16 Plunge.configure :default @16:51:27.27 SamplePump.configure :default @16:51:27.38 Toroid.configure :default @16:51:27.47 Carousel.configure :default @16:51:27.58 Abs.configure :default @16:51:27.69 PhaseSeparator.configure :default @16:51:27.79 Archive.configure :default @16:51:27.88 Air.configure :default @16:51:27.97 Lysis1.configure :default @16:51:28.06 Lysis2.configure :default @16:51:28.16 PinPort.configure :default @16:51:28.24 All elf controllers running firmware version 4.92 @16:51:28.27 Gate.power :motor,:ON,limit to 5000mA @16:51:28.47 Plunge.home.jog 0 @16:51:29.34 Lysis2.home.jog 0 @16:51:29.38 Air.home.jog 0 @16:51:29.42 Carousel.home.jog 0 @16:51:29.50 Toroid.home.jog 0 @16:51:29.54 Lysis1.home.jog 0 @16:51:29.59 Archive.home.jog 0 @16:51:29.64 PinPort.home.jog 0 @16:51:30.51 Plunge.in :homing @16:51:30.86 Plunge.jog 1607 @16:51:30.95 Lysis2.in :homing @16:51:31.01 Air.in :homing @16:51:31.04 Toroid.in :homing @16:51:31.09 PinPort.in :homing @16:51:31.12 Archive.in :homing @16:51:31.17 Lysis1.in :homing @16:51:31.96 Lysis2.jog 1403 @16:51:32.00 PinPort.jog -110 @16:51:32.07 Air.jog 1376 @16:51:32.13 Toroid.jog 627 @16:51:32.23 Archive.jog 1399 @16:51:32.33 Lysis1.jog 1400 @16:51:32.67 PinPort.setRawPosition! 0 @16:51:32.71 Plunge.setRawPosition! 50 @16:51:32.87 PinPort.seek :home @16:51:32.91 Plunge.seek :home @16:51:33.15 PinPort.in :default @16:51:33.32 Plunge.in :default @16:51:33.42 Lysis2.setRawPosition! 50 @16:51:33.50 Lysis2.seek :home @16:51:33.61 Air.setRawPosition! 50 @16:51:33.69 Air.seek :home @16:51:33.85 Lysis1.setRawPosition! 50 @16:51:33.88 Archive.setRawPosition! 50 @16:51:33.92 Lysis2.in :default @16:51:34.09 Archive.seek :home @16:51:34.16 Lysis1.seek :home @16:51:34.48 Air.in :default @16:51:34.63 Toroid.setRawPosition! 0 @16:51:34.66 Lysis1.in :default @16:51:34.81 Toroid.seek :home @16:51:34.97 Archive.in :default @16:51:34.99 Toroid.in :default @16:51:35.40 Toroid.seek :clear @16:51:37.13 Carousel.in :default @16:51:37.27 Carousel.home.seek 5 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 5 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:53:03.31 -> SC Storage Carousel (SC) at 5 @16:54:09.74 -> SC.to 35 @16:54:09.79 Carousel.seek 35 Storage Carousel (SC) at 35 @16:54:38.36 -> SC.to 45 @16:54:38.40 Carousel.seek 45 Storage Carousel (SC) at 45 @16:54:57.38 -> SC.to 55 @16:54:57.43 Carousel.seek 55 Storage Carousel (SC) at 55 @16:56:37.15 -> SC.to 60 @16:56:37.19 Carousel.seek 60 Storage Carousel (SC) at 60 @17:01:46.47 -> ESP.ready! @17:01:47.29 Toroid.seek :clear @17:01:47.48 Carousel.in :default @17:01:47.62 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:01:58.15 -> ESP.reready! @17:01:58.21 Plunge.in :default @17:01:58.30 Toroid.in :default @17:01:58.37 Carousel.in :default @17:01:58.47 Archive.in :default @17:01:58.62 Lysis1.in :default @17:01:58.79 Air.in :default @17:01:58.85 Plunge.home.jog 0 @17:01:58.89 Toroid.home.jog 0 @17:01:58.94 Lysis2.in :default @17:01:58.98 Carousel.home.jog 0 @17:01:59.02 PinPort.in :default @17:01:59.10 Archive.home.jog 0 @17:01:59.40 Lysis1.home.jog 0 @17:01:59.52 Air.home.jog 0 @17:01:59.60 Lysis2.home.jog 0 @17:01:59.67 PinPort.home.jog 0 @17:02:00.15 Plunge.in :homing @17:02:00.42 Plunge.jog 154 @17:02:00.87 Lysis1.in :homing @17:02:00.93 Toroid.in :homing @17:02:01.04 Archive.in :homing @17:02:01.17 Lysis1.jog 1399 @17:02:01.30 Archive.jog 1399 @17:02:01.38 Toroid.jog 626 @17:02:01.65 Plunge.setRawPosition! 50 @17:02:01.71 Lysis2.in :homing @17:02:01.75 PinPort.in :homing @17:02:01.82 Air.in :homing @17:02:02.01 Plunge.seek :home @17:02:02.07 PinPort.jog -111 @17:02:02.12 Lysis2.jog 1395 @17:02:02.21 Air.jog 1376 @17:02:02.54 PinPort.setRawPosition! 0 @17:02:02.57 Lysis1.setRawPosition! 50 @17:02:02.65 Plunge.in :default @17:02:02.76 PinPort.seek :home @17:02:02.87 Lysis1.seek :home @17:02:03.10 PinPort.in :default @17:02:03.13 Archive.setRawPosition! 50 @17:02:03.30 Archive.seek :home @17:02:03.44 Lysis1.in :default @17:02:03.58 Lysis2.setRawPosition! 50 @17:02:03.63 Air.setRawPosition! 50 @17:02:03.72 Lysis2.seek :home @17:02:03.82 Air.seek :home @17:02:04.00 Toroid.setRawPosition! 0 @17:02:04.04 Archive.in :default @17:02:04.19 Toroid.seek :home @17:02:04.36 Lysis2.in :default @17:02:04.41 Air.in :default @17:02:04.44 Toroid.in :default @17:02:04.88 Toroid.seek :clear @17:02:06.61 Carousel.in :default @17:02:06.79 Carousel.home.seek 60 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 60 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:02:13.09 -> SC.to 5 @17:02:13.14 Carousel.seek 5 Storage Carousel (SC) at 5 @17:02:31.15 -> SC.to 40 @17:02:31.19 Carousel.seek 40 Storage Carousel (SC) at 40 @17:17:47.91 -> slot {[32,30,28,26,24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @17:19:21.06 -> Cartridge.release 32 0 @17:21:57.13 -> PhytoSampler.xray @17:22:09.78 -> PhytoSampler.source=nil @17:23:03.88 -> PhytoSampler.goalVolume=100 100 @17:23:08.79 -> PhytoSampler.xray @17:25:52.36 -> fire lysis # @17:25:53.36 Toroid.seek :clear @17:25:53.57 Carousel.in :default @17:25:53.71 Carousel.home.seek 40 @17:25:53.99 Selecting Cartridge 32 @17:25:54.31 Carousel.seek 32 @17:25:56.30 Lysis1.in :fast @17:25:56.38 Lysis1.seek :locked @17:25:59.04 Plunge.seek :locked @17:25:59.24 Air.in :fast @17:25:59.33 Air.seek :locked @17:26:00.34 Carousel.reconfigure :locked @17:26:00.53 Powering cartridge bus @17:26:03.19 Gate.setIR 1750,5000,0,6 @17:26:03.32 Gate.setIR 0,5000,0,6 @17:26:03.41 PuckHeater.configure :default @17:26:03.50 Gate.power :heat,:ON,limit to 5000mA @17:26:04.27 Mounted lysis Cartridge board#Q05034 @17:26:04.33 Using this cartridge's heat loss curve for HeatingAccelInCarousel @17:26:04.39 GoodNews.email "PhytoSampler filtering up to 100 ml",Subject: "Sampling microSPR_phyto cartridge in slot 32" @17:26:04.82 Preparing to filter cartridge 32 @17:26:05.15 Air.in :default @17:26:05.38 Priming sample loop w/150ml, bypass w/50ml @17:26:05.41 Air.seek :sealed @17:26:05.47 SamplePump.setPosition! 0ml @17:26:05.64 Bypass.open @17:26:07.30 Intake.open @17:26:08.16 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:26:10.82 Vacuum tare is 52% of full scale at 23C (drifted 1%) @17:26:11.02 Abs.in :default @17:26:11.10 Abs.hold @17:26:11.44 SamplePump.in :start @17:26:11.55 SamplePump.seek 50ml,3:50 @17:26:11.90 upsync @17:26:12.13 SamplePump.configure :prime @17:27:12.94 Abs.stop @17:27:13.05 SamplePump.setPosition! 0ml @17:27:13.12 Exhaust.open @17:27:14.52 Bypass.close @17:27:16.04 Abs.in :default @17:27:16.13 Abs.hold @17:27:16.50 SamplePump.in :start @17:27:16.59 SamplePump.seek 150ml,10:30 @17:27:17.20 SamplePump.configure :prime @17:30:19.94 Abs.stop @17:30:20.06 Bypass.open @17:30:20.77 Intake.close @17:30:21.59 Toroid.in :hold @17:30:21.67 Toroid.seek :crack @17:30:23.25 Toroid.seek :cartridge @17:30:24.44 Toroid.in :default @17:30:24.55 Toroid.seek :clear @17:30:26.64 Pressurizing cartridge to 26psi above 14.9psia @17:30:26.66 SamplePump.setPosition! 0ml @17:30:26.84 Intake.open @17:30:27.58 Bypass.close @17:30:28.31 Exhaust.close @17:30:29.17 Abs.in :default @17:30:29.25 Abs.hold @17:30:29.55 SamplePump.in :charge @17:30:29.66 SamplePump.seek 2.3ml,42 seconds @17:30:46.23 Abs.stop @17:30:46.33 Intake.close @17:30:47.13 Pressurized cartridge with 1.155ml to 26.1psi @17:30:47.14 Isolated @35.4psia (20.5psi) @17:30:47.17 Verifying cartridge holds at least 0.65..0.85 of 35.4psia @17:30:47.24 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:30:51.85 Cartridge pressure fell 5% to 33.6psia @17:30:51.98 Sampling 100.0ml @17:30:52.00 SamplePump.setPosition! 0ml @17:30:52.09 Exhaust.open @17:30:52.77 Intake.open @17:30:53.71 Abs.in :default @17:30:53.80 Abs.hold @17:30:54.17 SamplePump.in :start @17:30:54.28 SamplePump.seek 100ml,8:50 @17:30:54.86 SamplePump.configure :sample @17:30:57.34 Cartridge 32 filtering 2/100ml at 0.74ml/s with 25.8psi vacuum @17:31:57.36 Cartridge 32 filtering 42/100ml at 0.67ml/s with 11.8psi vacuum 3.6m deep (20.1psia) @17:32:57.36 Cartridge 32 filtering 78/100ml at 0.60ml/s with 26.2psi vacuum @17:33:36.77 Abs.stop @17:33:36.96 Intake.close @17:33:37.01 Exhaust.close @17:33:37.33 Toroid.in :hold @17:33:37.42 Toroid.seek :bypass @17:33:39.08 Toroid.in :default @17:33:39.18 Toroid.seek :clear @17:33:40.83 Sampled 100.0ml @17:33:41.26 GoodNews.email "After filtering 100.0ml",Subject: "Processing microSPR_phyto cartridge in slot 32" @17:33:42.92 SPR.command "read_sequences" @17:33:43.61 SPR.command "stats_on" @17:33:44.40 SPR.command "start 0 1" @17:33:45.09 SPR.start /var/log/esp/spr24aug1917h.spr.bz2 @17:33:46.67 upsync @17:33:47.13 Handoff.select :waste @17:33:47.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:33:53.93 Syringe tare is 52% of full scale at 23C (drifted 1%) @17:33:53.98 PinPort.in :locking @17:33:54.07 PinPort.seek :locked @17:33:54.61 Archive.in :fast @17:33:54.76 Lysis2.in :fast @17:33:54.82 Lysis1.seek 1350uL @17:33:54.92 Archive.seek :full @17:33:55.07 Lysis2.seek 950uL @17:33:59.95 Plunge.seek :reagent @17:34:00.94 Evacuating puck with filter bubble point of 24psi @17:34:00.98 PinPort.seek :locked @17:34:01.12 Handoff.select :waste @17:34:01.17 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:34:07.77 Air syringe pumping up to 1 strokes to reach 8psi @17:34:07.78 Air stroke #1 of 1 @17:34:07.92 Air.in :pressurize @17:34:08.00 Air.seek :pressurized @17:34:13.80 1.6psi after pressurize @17:34:13.83 Air.in :compress @17:34:13.94 Air.seek :evac @17:34:17.03 2.2psi after compress @17:34:29.14 1.1psi after compress (stable) @17:34:29.15 Air.in :default @17:34:29.28 Air.hold @17:34:29.54 at 0.7psi Opened to waste @17:34:29.55 Handoff.select :blocked @17:34:29.72 Archive syringe pumping up to 8 strokes to reach 24psi @17:34:29.74 Archive stroke #1 of 8 @17:34:29.87 Archive.in :pressurize @17:34:29.96 Archive.seek :pressurized @17:34:42.43 2.6psi after pressurize @17:34:48.05 2.1psi after pressurize (stable) @17:34:48.08 Archive.in :compress @17:34:48.21 Archive.seek :empty @17:35:03.97 14.6psi after compress @17:35:14.56 14.7psi after compress (stable) @17:35:14.62 Archive.in :gulp @17:35:14.77 Archive.seek :topVent @17:35:21.10 15.4psi after gulp @17:35:26.69 14.9psi after gulp (stable) @17:35:26.70 Archive stroke #2 of 8 @17:35:26.87 Archive.in :pressurize @17:35:27.40 Archive.seek :pressurized @17:35:39.96 15.3psi after pressurize @17:35:45.55 14.6psi after pressurize (stable) @17:35:45.58 Archive.in :compress @17:35:45.69 Archive.seek :empty @17:35:58.04 Reached 24.1 of 24.0psi after pumping 3387uL (1.96 strokes) @17:35:58.07 Archive.in :default @17:35:58.21 Archive.hold @17:35:58.32 Handoff.select :waste @17:35:58.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:36:09.62 delay 1:00 @17:37:09.73 Handoff.select :blocked @17:37:09.91 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:37:32.50 Handoff.select :waste @17:37:32.66 0.5psi at evacEnd @17:37:32.67 Handoff.select :blocked @17:37:32.91 Archive.in :fast @17:37:32.99 Archive.seek :topVent @17:37:39.94 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @17:37:46.53 Stable @0.4psi after Handoff blocked @17:37:46.56 Archive syringe pumping up to 8 strokes to reach 24psi @17:37:46.60 Archive stroke #1 of 8 @17:37:46.76 Archive.in :pressurize @17:37:46.85 Archive.seek :pressurized @17:37:59.33 1.1psi after pressurize @17:37:59.36 Archive.in :compress @17:37:59.47 Archive.seek :empty @17:38:15.22 13.9psi after compress @17:38:25.82 13.0psi after compress (stable) @17:38:25.84 Archive.in :gulp @17:38:25.96 Archive.seek :topVent @17:38:32.32 14.0psi after gulp @17:38:37.96 13.4psi after gulp (stable) @17:38:38.00 Archive stroke #2 of 8 @17:38:38.16 Archive.in :pressurize @17:38:38.24 Archive.seek :pressurized @17:38:50.74 13.8psi after pressurize @17:38:50.77 Archive.in :compress @17:38:50.89 Archive.seek :empty @17:39:04.59 Reached 24.7 of 24.4psi after pumping 3414uL (1.97 strokes) @17:39:04.62 Archive.in :default @17:39:04.75 Archive.hold @17:39:04.87 Handoff.select :waste @17:39:05.04 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:39:14.14 delay 1:00 @17:40:14.17 Handoff.select :blocked @17:40:14.34 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:40:37.01 Handoff.select :waste @17:40:37.28 0.5psi at evacEnd @17:40:37.31 Air.seek :fullVent @17:40:38.67 Archive.in :fast @17:40:38.80 Archive.seek :topVent @17:40:45.82 Seawater evacuated, Pressure above/below filter released. @17:40:45.94 Lysis1.in :slow @17:40:46.03 Lysis1.seek 1050uL @17:41:15.69 delay 5 seconds @17:41:20.72 Handoff.select :blocked @17:41:20.98 Lysis1.seek 450uL @17:42:20.16 delay 3 seconds @17:42:23.62 Air.in mix with 1.57127 parts Lysis1 @17:42:24.37 Air.seek :evac @17:42:24.44 Lysis1.seek 300uL @17:42:39.42 Lysis1.seek :empty @17:43:09.14 Lysis buffer delivered @17.0psi @17:43:09.16 Polling Can every 2:00 @17:43:09.74 PuckHeater.configure :default #ambient 23.8C @17:43:09.82 PuckHeater.seek 80.6C @17:46:20.24 PuckHeater.hold 2:00,80.6C @17:48:20.55 Polling Can every 2:00 @17:48:21.11 PuckHeater.configure :default #ambient 23.8C @17:48:21.20 PuckHeater.hold 30 seconds,79.5C @17:48:51.49 Polling Can every 2:00 @17:48:52.11 PuckHeater.configure :default #ambient 23.8C @17:48:52.20 PuckHeater.hold 30 seconds,79C @17:49:22.48 Polling Can every 2:00 @17:49:23.06 PuckHeater.configure :default #ambient 23.8C @17:49:23.18 PuckHeater.hold 10:00,78.5C @17:59:24.36 Lysis complete. Syringe pressure @15.3psi @17:59:24.39 Archive syringe pumping up to 5 strokes to reach 40psi @17:59:24.43 Archive stroke #1 of 5 @17:59:24.59 Archive.in :pressurize @17:59:24.70 Archive.seek :pressurized @17:59:37.18 15.4psi after pressurize @17:59:42.81 14.8psi after pressurize (stable) @17:59:42.83 Archive.in :compress @17:59:42.94 Archive.seek :empty @17:59:52.99 Reached 40.4 of 40.0psi after pumping 1607uL (0.93 strokes) @17:59:53.01 Archive.in :default @17:59:53.12 Archive.hold @17:59:53.23 delay 2 seconds @17:59:55.26 Archive.in :fast @17:59:55.34 Archive.seek :full @18:00:02.17 Lysis1.seek 138uL @18:00:15.89 delay 1 second @18:00:16.93 Archive.seek :full @18:00:17.04 Lysis1.seek :empty @18:00:30.81 Handoff.select :blocked @18:00:30.99 Initial air pressure @42.6psi @18:00:31.09 Lysis1.in :extract @18:00:31.19 Lysis1.seek 600uL,nil @18:00:33.72 Archive syringe pumping up to 6 strokes to reach 25psi @18:00:33.74 Archive stroke #1 of 6 @18:00:33.93 Archive.in :pressurize @18:00:34.04 Archive.seek :pressurized @18:00:46.53 14.6psi after pressurize @18:00:52.30 14.2psi after pressurize (stable) @18:00:52.46 Archive.in :compress @18:00:52.61 Archive.seek :empty @18:01:00.67 Archive.in :default @18:01:00.74 Archive.hold @18:01:00.97 Initial 600uL lysate @15.6psi @18:01:00.99 Handoff.select :blocked @18:01:01.17 Initial air pressure @15.8psi @18:01:01.27 Lysis1.seek 780uL,nil @18:01:02.67 Archive syringe pumping up to 6 strokes to reach 17psi @18:01:02.69 Archive stroke #1 of 6 @18:01:02.95 Archive.in :compress @18:01:03.09 Archive.seek :empty @18:01:05.83 Archive.in :default @18:01:05.90 Archive.hold @18:01:06.21 Recovered 780uL lysate @15.8psi @18:01:06.24 Air.in :halfSpeed @18:01:06.35 Air.seek :fullVent @18:01:08.96 Puck Top Vented, bottom @17.8psi @18:01:09.08 Handoff.select :waste @18:01:09.25 delay 5 seconds @18:01:14.38 Handoff open to waste, bottom @0.8psi @18:01:14.41 Air.in :default @18:01:14.53 Air.seek :evac @18:01:15.87 Lysis2.in :DAmix @18:01:16.02 Lysis1.in :DAmix @18:01:16.68 Lysis2.seek 800uL @18:01:16.73 Lysis1.seek 630uL @18:01:37.67 delay 5 seconds @18:01:42.70 Handoff.select SPR @18:01:42.89 SPR.command "start 2 14" @18:01:44.60 SPR stabilized @18:01:44.82 delay 10 seconds @18:01:54.84 SPR.command "start 2 0" @18:01:57.98 Waiting up to 11:00 for SPR to enter state 1 @18:03:56.06 SPR.command "hold_on" @18:03:57.46 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @18:03:58.27 Lysis2.seek 350uL @18:03:58.34 Lysis1.seek 180uL @18:05:00.56 delay 15 seconds @18:05:15.58 SPR.command "hold_off" @18:05:18.92 Waiting up to 1:00:00 for SPR to enter state 7 @18:21:34.47 SPRsummary:-31.7RIU,227RIU,266RIU,-30.8RIU,none,none,high after 3031s @18:35:51.37 SPR analysis completed @18:36:01.57 Ended log spr24aug1917h.spr.bz2 @18:36:01.59 SPR.command "stats_off" @18:36:02.39 Handoff.select :close @18:36:02.56 Gate.power :heat,:OFF @18:36:02.58 Gate.power :cartridge,:OFF @18:36:02.69 PinPort.in :unlocking @18:36:02.79 PinPort.seek :clear @18:36:03.30 Carousel.in :default @18:36:03.44 Carousel.home.seek 32 @18:36:04.31 Plunge.seek :locked @18:36:05.24 Archive.in :unlocking @18:36:05.42 Archive.seek :clear @18:36:05.49 Air.in :unlocking @18:36:05.70 Air.seek :clear @18:36:05.85 Lysis1.in :unlocking @18:36:06.01 Lysis1.seek :clear @18:36:06.08 Lysis2.in :unlocking @18:36:06.24 Lysis2.seek :clear @18:36:10.41 Gate.power :analytic1,:OFF @18:36:10.43 Gate.power :motor,:OFF @18:36:10.47 Gate.power :core,:OFF @18:36:10.50 Gate.power :main,:OFF @18:36:10.55 Safely stopped and ready to power off @19:02:18.42 -> PhytoSampler.xrat @19:02:18.46 NoMethodError in bill -- undefined method `xrat' for PhytoSampler:Cartridge::Sampler @19:02:23.52 -> PhytoSampler.xray @19:02:56.68 -> PhytoSampler.goalVolume=1000 1000 @19:03:11.60 -> PhytoSampler.xray @19:04:55.47 -> fire lysis # @19:04:55.73 Gate.power :sampler,:OFF,limit to 3500mA @19:04:55.76 Gate.power :skimmer,:OFF,limit to 3500mA @19:04:55.81 Gate.power :main,:ON,limit to 5000mA @19:04:55.85 Gate.power :analytic1,:ON,limit to 4000mA @19:04:55.96 Powering core bus @19:04:58.31 SPR Module 1, FW Rev. B, FPGA Rev. C @19:04:58.77 Plunge.configure :default @19:04:58.89 SamplePump.configure :default @19:04:58.99 Toroid.configure :default @19:04:59.10 Carousel.configure :default @19:04:59.20 Abs.configure :default @19:04:59.31 PhaseSeparator.configure :default @19:04:59.41 Archive.configure :default @19:04:59.52 Air.configure :default @19:04:59.60 Lysis1.configure :default @19:04:59.69 Lysis2.configure :default @19:04:59.79 PinPort.configure :default @19:04:59.86 All elf controllers running firmware version 4.92 @19:04:59.89 Gate.power :motor,:ON,limit to 5000mA @19:05:00.15 Plunge.home.jog 0 @19:05:00.99 PinPort.home.jog 0 @19:05:01.03 Lysis1.home.jog 0 @19:05:01.08 Archive.home.jog 0 @19:05:01.13 Toroid.home.jog 0 @19:05:01.20 Lysis2.home.jog 0 @19:05:01.22 Air.home.jog 0 @19:05:01.28 Carousel.home.jog 0 @19:05:02.19 Plunge.in :homing @19:05:02.94 Lysis2.in :homing @19:05:03.13 Lysis1.in :homing @19:05:03.18 Archive.in :homing @19:05:03.22 PinPort.in :homing @19:05:03.26 Air.in :homing @19:05:03.30 Plunge.jog 1610 @19:05:03.35 Toroid.in :homing @19:05:03.60 Lysis2.jog 1402 @19:05:03.79 PinPort.jog -110 @19:05:03.88 Lysis1.jog 1399 @19:05:03.91 Air.jog 1382 @19:05:03.95 Archive.jog 1406 @19:05:03.99 Toroid.jog 624 @19:05:04.44 PinPort.setRawPosition! 0 @19:05:04.52 PinPort.seek :home @19:05:04.67 PinPort.in :default @19:05:04.86 Lysis2.setRawPosition! 50 @19:05:04.96 Lysis2.seek :home @19:05:05.08 Plunge.setRawPosition! 50 @19:05:05.17 Plunge.seek :home @19:05:05.31 Lysis1.setRawPosition! 50 @19:05:05.35 Lysis2.in :default @19:05:05.49 Lysis1.seek :home @19:05:05.66 Air.setRawPosition! 50 @19:05:05.70 Archive.setRawPosition! 50 @19:05:05.74 Plunge.in :default @19:05:06.08 Air.seek :home @19:05:06.11 Archive.seek :home @19:05:06.34 Lysis1.in :default @19:05:06.49 Toroid.setRawPosition! 0 @19:05:06.53 Air.in :default @19:05:06.65 Toroid.seek :home @19:05:06.87 Archive.in :default @19:05:06.89 Toroid.in :default @19:05:07.31 Toroid.seek :clear @19:05:09.03 Carousel.in :default @19:05:09.18 Carousel.home.seek 32 @19:05:09.54 Selecting Cartridge 30 @19:05:09.86 Carousel.seek 30 @19:05:10.63 Lysis1.in :fast @19:05:10.74 Lysis1.seek :locked @19:05:13.41 Plunge.seek :locked @19:05:13.60 Air.in :fast @19:05:13.67 Air.seek :locked @19:05:14.67 Carousel.reconfigure :locked @19:05:14.88 Powering cartridge bus @19:05:17.55 Gate.setIR 1750,5000,0,6 @19:05:17.68 Gate.setIR 0,5000,0,6 @19:05:17.77 PuckHeater.configure :default #ambient 23.8C @19:05:17.86 Gate.power :heat,:ON,limit to 5000mA @19:05:19.02 Mounted lysis Cartridge board#Q05064 @19:05:19.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:05:19.23 GoodNews.email "PhytoSampler filtering up to 1000 ml",Subject: "Sampling microSPR_phyto cartridge in slot 30" @19:05:19.63 Preparing to filter cartridge 30 @19:05:19.98 Air.in :default @19:05:20.16 Air.seek :sealed @19:05:20.31 Priming sample loop w/150ml, bypass w/50ml @19:05:20.34 SamplePump.setPosition! 0ml @19:05:20.43 Bypass.open @19:05:21.91 Intake.open @19:05:22.90 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:05:24.98 upsync @19:05:25.54 Vacuum tare is 52% of full scale at 24C (drifted 1%) @19:05:25.75 Abs.in :default @19:05:25.84 Abs.hold @19:05:26.11 SamplePump.in :start @19:05:26.21 SamplePump.seek 50ml,3:50 @19:05:26.80 SamplePump.configure :prime @19:06:27.61 Abs.stop @19:06:27.69 SamplePump.setPosition! 0ml @19:06:27.77 Exhaust.open @19:06:29.16 Bypass.close @19:06:30.68 Abs.in :default @19:06:30.81 Abs.hold @19:06:31.16 SamplePump.in :start @19:06:31.27 SamplePump.seek 150ml,10:30 @19:06:31.85 SamplePump.configure :prime @19:09:34.79 Abs.stop @19:09:34.94 Bypass.open @19:09:35.61 Intake.close @19:09:36.44 Toroid.in :hold @19:09:36.52 Toroid.seek :crack @19:09:38.61 Toroid.seek :cartridge @19:09:39.85 Toroid.in :default @19:09:39.95 Toroid.seek :clear @19:09:42.03 Pressurizing cartridge to 26psi above 15.0psia @19:09:42.05 SamplePump.setPosition! 0ml @19:09:42.14 Intake.open @19:09:42.87 Bypass.close @19:09:43.56 Exhaust.close @19:09:44.34 Abs.in :default @19:09:44.42 Abs.hold @19:09:44.68 SamplePump.in :charge @19:09:44.81 SamplePump.seek 2.3ml,42 seconds @19:09:59.53 Abs.stop @19:09:59.63 Intake.close @19:10:00.45 Pressurized cartridge with 0.972ml to 26.2psi @19:10:00.47 Isolated @38.7psia (23.8psi) @19:10:00.50 Verifying cartridge holds at least 0.65..0.85 of 38.7psia @19:10:00.56 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:10:05.18 Cartridge pressure fell 2% to 37.9psia @19:10:05.30 Sampling 1000.0ml @19:10:05.32 SamplePump.setPosition! 0ml @19:10:05.41 Exhaust.open @19:10:06.10 Intake.open @19:10:07.02 Abs.in :default @19:10:07.10 Abs.hold @19:10:07.46 SamplePump.in :start @19:10:07.56 SamplePump.seek 1000ml,1:23:50 @19:10:08.15 SamplePump.configure :sample @19:10:10.55 Cartridge 30 filtering 2/1000ml at 0.82ml/s with 14.9psi vacuum 8.4m deep (27.1psia) @19:11:10.61 Cartridge 30 filtering 42/1000ml at 0.67ml/s with 24.8psi vacuum @19:12:10.61 Cartridge 30 filtering 80/1000ml at 0.63ml/s with 21.8psi vacuum @19:13:10.61 Cartridge 30 filtering 116/1000ml at 0.60ml/s with 18.9psi vacuum 6.8m deep (24.8psia) @19:14:10.61 Cartridge 30 filtering 150/1000ml at 0.56ml/s with 21.4psi vacuum @19:15:10.61 Cartridge 30 filtering 180/1000ml at 0.51ml/s with 21.1psi vacuum 4.6m deep (21.5psia) @19:16:11.08 Cartridge 30 filtering 207/1000ml at 0.44ml/s with 26.9psi vacuum @19:17:10.61 Cartridge 30 filtering 228/1000ml at 0.36ml/s with 21.5psi vacuum @19:18:10.61 Cartridge 30 filtering 245/1000ml at 0.28ml/s with 23.2psi vacuum @19:19:10.61 Cartridge 30 filtering 257/1000ml at 0.21ml/s with 23.4psi vacuum @19:20:10.61 Cartridge 30 filtering 267/1000ml at 0.16ml/s with 22.7psi vacuum @19:20:10.68 SamplePump.stop @19:20:10.80 Intake.close @19:20:10.90 Exhaust.close @19:20:11.21 Toroid.in :hold @19:20:11.30 Toroid.seek :bypass @19:20:12.98 Toroid.in :default @19:20:13.08 Toroid.seek :clear @19:20:14.76 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @19:20:14.78 Abs.stop @19:20:15.08 Sampled 266.9ml @19:20:15.56 GoodNews.email "After filtering 266.9ml",Subject: "Processing microSPR_phyto cartridge in slot 30" @19:20:17.20 SPR.command "read_sequences" @19:20:17.55 SPR.command "stats_on" @19:20:17.76 SPR.command "start 0 1" @19:20:18.45 SPR.start /var/log/esp/spr24aug1919h.spr.bz2 @19:20:20.40 Handoff.select :waste @19:20:20.62 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:20:21.11 upsync @19:20:27.79 Syringe tare is 51% of full scale at 24C (drifted 0%) @19:20:27.87 PinPort.in :locking @19:20:27.98 PinPort.seek :locked @19:20:28.52 Archive.in :fast @19:20:28.59 Lysis2.in :fast @19:20:28.67 Lysis1.seek 1350uL @19:20:28.83 Archive.seek :full @19:20:28.90 Lysis2.seek 950uL @19:20:33.75 Plunge.seek :reagent @19:20:34.69 Evacuating puck with filter bubble point of 24psi @19:20:34.75 PinPort.seek :locked @19:20:34.89 Handoff.select :waste @19:20:34.94 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:20:41.54 Air syringe pumping up to 1 strokes to reach 8psi @19:20:41.55 Air stroke #1 of 1 @19:20:41.68 Air.in :pressurize @19:20:41.78 Air.seek :pressurized @19:20:47.47 0.8psi after pressurize @19:20:47.51 Air.in :compress @19:20:47.61 Air.seek :evac @19:20:50.65 2.1psi after compress @19:21:08.78 0.8psi after compress (stable) @19:21:08.80 Air.in :default @19:21:08.90 Air.hold @19:21:09.19 at 0.7psi Opened to waste @19:21:09.20 Handoff.select :blocked @19:21:09.39 Archive syringe pumping up to 8 strokes to reach 24psi @19:21:09.40 Archive stroke #1 of 8 @19:21:09.52 Archive.in :pressurize @19:21:09.61 Archive.seek :pressurized @19:21:22.08 3.7psi after pressurize @19:21:27.67 3.2psi after pressurize (stable) @19:21:27.71 Archive.in :compress @19:21:27.80 Archive.seek :empty @19:21:43.55 21.3psi after compress @19:21:56.14 23.7psi after compress (stable) @19:21:56.18 Archive.in :gulp @19:21:56.28 Archive.seek :topVent @19:21:56.55 Reached 24.4 of 24.0psi after pumping 1730uL (1.00 strokes) @19:21:56.57 Archive.in :default @19:21:56.68 Archive.hold @19:21:56.82 Handoff.select :waste @19:21:56.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:22:14.09 delay 1:00 @19:23:14.11 Handoff.select :blocked @19:23:14.29 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:23:36.89 Handoff.select :waste @19:23:37.06 0.9psi at evacEnd @19:23:37.07 Handoff.select :blocked @19:23:37.26 Archive.in :fast @19:23:37.33 Archive.seek :topVent @19:23:44.52 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @19:23:51.11 Stable @0.7psi after Handoff blocked @19:23:51.14 Archive syringe pumping up to 8 strokes to reach 25psi @19:23:51.17 Archive stroke #1 of 8 @19:23:51.31 Archive.in :pressurize @19:23:51.40 Archive.seek :pressurized @19:24:03.87 1.5psi after pressurize @19:24:03.91 Archive.in :compress @19:24:04.01 Archive.seek :empty @19:24:19.76 16.4psi after compress @19:24:30.35 16.6psi after compress (stable) @19:24:30.38 Archive.in :gulp @19:24:30.49 Archive.seek :topVent @19:24:36.84 17.6psi after gulp @19:24:42.96 16.9psi after gulp (stable) @19:24:42.98 Archive stroke #2 of 8 @19:24:43.14 Archive.in :pressurize @19:24:43.22 Archive.seek :pressurized @19:24:55.68 17.4psi after pressurize @19:24:55.71 Archive.in :compress @19:24:55.81 Archive.seek :empty @19:25:07.12 Reached 25.0 of 24.7psi after pumping 3364uL (1.94 strokes) @19:25:07.14 Archive.in :default @19:25:07.25 Archive.hold @19:25:07.35 Handoff.select :waste @19:25:07.51 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:25:24.60 delay 1:00 @19:26:24.62 Handoff.select :blocked @19:26:24.88 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:26:47.47 Handoff.select :waste @19:26:47.62 0.5psi at evacEnd @19:26:47.65 Air.seek :fullVent @19:26:49.03 Archive.in :fast @19:26:49.13 Archive.seek :topVent @19:26:55.97 Seawater evacuated, Pressure above/below filter released. @19:26:56.10 Lysis1.in :slow @19:26:56.18 Lysis1.seek 1050uL @19:27:25.85 delay 5 seconds @19:27:30.87 Handoff.select :blocked @19:27:31.11 Lysis1.seek 450uL @19:28:30.31 delay 3 seconds @19:28:33.41 Air.in mix with 1.57127 parts Lysis1 @19:28:34.17 Air.seek :evac @19:28:34.23 Lysis1.seek 300uL @19:28:49.24 Lysis1.seek :empty @19:29:18.99 Lysis buffer delivered @23.1psi @19:29:19.01 Polling Can every 2:00 @19:29:19.60 PuckHeater.configure :default #ambient 24.3C @19:29:19.68 PuckHeater.seek 79.6C @19:30:15.22 PuckHeater.hold 2:00,79.6C @19:32:15.04 Polling Can every 2:00 @19:32:15.59 PuckHeater.configure :default #ambient 24.4C @19:32:15.68 PuckHeater.hold 30 seconds,78.6C @19:32:45.83 Polling Can every 2:00 @19:32:46.38 PuckHeater.configure :default #ambient 24.4C @19:32:46.46 PuckHeater.hold 30 seconds,78.1C @19:33:16.61 Polling Can every 2:00 @19:33:17.16 PuckHeater.configure :default #ambient 24.4C @19:33:17.26 PuckHeater.hold 10:00,77.6C @19:43:15.80 Lysis complete. Syringe pressure @28.4psi @19:43:15.84 Archive syringe pumping up to 5 strokes to reach 40psi @19:43:15.87 Archive stroke #1 of 5 @19:43:16.00 Archive.in :pressurize @19:43:16.08 Archive.seek :pressurized @19:43:28.57 28.5psi after pressurize @19:43:34.20 27.8psi after pressurize (stable) @19:43:34.22 Archive.in :compress @19:43:34.32 Archive.seek :empty @19:43:43.57 Reached 40.7 of 40.0psi after pumping 1591uL (0.92 strokes) @19:43:43.62 Archive.in :default @19:43:43.73 Archive.hold @19:43:43.84 delay 2 seconds @19:43:45.88 Archive.in :fast @19:43:45.95 Archive.seek :full @19:43:52.76 Lysis1.seek 138uL @19:44:06.48 delay 1 second @19:44:07.52 Archive.seek :full @19:44:07.56 Lysis1.seek :empty @19:44:21.35 Handoff.select :blocked @19:44:21.57 Initial air pressure @44.3psi @19:44:21.67 Lysis1.in :extract @19:44:21.78 Lysis1.seek 600uL,nil @19:44:24.90 Archive syringe pumping up to 6 strokes to reach 25psi @19:44:24.92 Archive stroke #1 of 6 @19:44:25.06 Archive.in :pressurize @19:44:25.19 Archive.seek :pressurized @19:44:37.69 13.2psi after pressurize @19:44:43.28 12.4psi after pressurize (stable) @19:44:43.30 Archive.in :compress @19:44:43.40 Archive.seek :empty @19:44:52.00 Archive.in :default @19:44:52.07 Archive.hold @19:44:52.28 Initial 600uL lysate @18.9psi @19:44:52.30 Handoff.select :blocked @19:44:52.44 Initial air pressure @18.3psi @19:44:52.54 Lysis1.seek 780uL,nil @19:44:53.93 Archive syringe pumping up to 6 strokes to reach 17psi @19:44:53.94 Archive stroke #1 of 6 @19:44:54.09 Archive.in :compress @19:44:54.16 Archive.seek :empty @19:44:54.59 Archive.in :default @19:44:54.65 Archive.hold @19:44:55.00 Recovered 780uL lysate @17.1psi @19:44:55.03 Air.in :halfSpeed @19:44:55.14 Air.seek :fullVent @19:44:57.17 Puck Top Vented, bottom @18.6psi @19:44:57.19 Handoff.select :waste @19:44:57.37 delay 5 seconds @19:45:02.45 Handoff open to waste, bottom @0.1psi @19:45:02.48 Air.in :default @19:45:02.59 Air.seek :evac @19:45:03.94 Lysis2.in :DAmix @19:45:04.14 Lysis1.in :DAmix @19:45:04.94 Lysis2.seek 800uL @19:45:05.00 Lysis1.seek 630uL @19:45:25.89 delay 5 seconds @19:45:30.91 Handoff.select SPR @19:45:31.09 SPR.command "start 2 14" @19:45:32.37 SPR stabilized @19:45:32.55 delay 10 seconds @19:45:42.57 SPR.command "start 2 0" @19:45:45.74 Waiting up to 11:00 for SPR to enter state 1 @19:47:43.83 SPR.command "hold_on" @19:47:45.23 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @19:47:45.99 Lysis2.seek 350uL @19:47:46.02 Lysis1.seek 180uL @19:48:48.24 delay 15 seconds @19:49:03.27 SPR.command "hold_off" @19:49:06.69 Waiting up to 1:00:00 for SPR to enter state 7 @20:05:22.26 SPRsummary:-10.8RIU,31.2RIU,42.8RIU,-10.2RIU,78.2ng/L,61.1ng/L,high after 2856s @20:19:39.15 SPR analysis completed @20:19:47.52 Ended log spr24aug1919h.spr.bz2 @20:19:47.54 SPR.command "stats_off" @20:19:48.27 Handoff.select :close @20:19:48.45 Gate.power :heat,:OFF @20:19:48.47 Gate.power :cartridge,:OFF @20:19:48.59 PinPort.in :unlocking @20:19:48.67 PinPort.seek :clear @20:19:49.21 Carousel.in :default @20:19:49.35 Carousel.home.seek 30 @20:19:50.17 Plunge.seek :locked @20:19:51.12 Archive.in :unlocking @20:19:51.28 Archive.seek :clear @20:19:51.40 Air.in :unlocking @20:19:51.58 Air.seek :clear @20:19:51.74 Lysis1.in :unlocking @20:19:51.84 Lysis1.seek :clear @20:19:51.98 Lysis2.in :unlocking @20:19:52.08 Lysis2.seek :clear @20:19:56.13 Gate.power :analytic1,:OFF @20:19:56.15 Gate.power :motor,:OFF @20:19:56.18 Gate.power :core,:OFF @20:19:56.22 Gate.power :main,:OFF @20:19:56.25 Safely stopped and ready to power off @12:22:13.12UTC20-Aug-24 -> PhytoSampler.xray @12:22:52.70 -> PhytoSampler.goalVolume=175 175 @12:25:39.48 -> fire lysis # @12:25:39.71 Gate.power :sampler,:OFF,limit to 3500mA @12:25:39.75 Gate.power :skimmer,:OFF,limit to 3500mA @12:25:39.79 Gate.power :main,:ON,limit to 5000mA @12:25:39.82 Gate.power :analytic1,:ON,limit to 4000mA @12:25:39.93 Powering core bus @12:25:42.27 SPR Module 1, FW Rev. B, FPGA Rev. C @12:25:42.72 Plunge.configure :default @12:25:42.86 SamplePump.configure :default @12:25:42.97 Toroid.configure :default @12:25:43.08 Carousel.configure :default @12:25:43.19 Abs.configure :default @12:25:43.28 PhaseSeparator.configure :default @12:25:43.39 Archive.configure :default @12:25:43.49 Air.configure :default @12:25:43.58 Lysis1.configure :default @12:25:43.66 Lysis2.configure :default @12:25:43.77 PinPort.configure :default @12:25:43.84 All elf controllers running firmware version 4.92 @12:25:43.86 Gate.power :motor,:ON,limit to 5000mA @12:25:44.16 Plunge.home.jog 0 @12:25:44.93 PinPort.home.jog 0 @12:25:44.96 Lysis1.home.jog 0 @12:25:45.04 Archive.home.jog 0 @12:25:45.08 Toroid.home.jog 0 @12:25:45.12 Lysis2.home.jog 0 @12:25:45.18 Air.home.jog 0 @12:25:45.22 Carousel.home.jog 0 @12:25:46.04 Plunge.in :homing @12:25:46.29 Plunge.jog 1599 @12:25:46.57 Lysis1.in :homing @12:25:46.63 Air.in :homing @12:25:46.68 Lysis2.in :homing @12:25:46.74 Archive.in :homing @12:25:46.80 PinPort.in :homing @12:25:46.84 Toroid.in :homing @12:25:47.15 Lysis2.jog 1406 @12:25:47.23 Lysis1.jog 1397 @12:25:47.25 Air.jog 1379 @12:25:47.30 PinPort.jog -111 @12:25:47.36 Archive.jog 1402 @12:25:47.45 Toroid.jog 623 @12:25:47.85 Plunge.setRawPosition! 50 @12:25:47.94 Plunge.seek :home @12:25:48.07 PinPort.setRawPosition! 0 @12:25:48.63 PinPort.seek :home @12:25:48.90 PinPort.in :default @12:25:48.98 Lysis2.setRawPosition! 50 @12:25:49.01 Lysis1.setRawPosition! 50 @12:25:49.05 Archive.setRawPosition! 50 @12:25:49.10 Plunge.in :default @12:25:49.14 Air.setRawPosition! 50 @12:25:49.46 Lysis2.seek :home @12:25:49.49 Lysis1.seek :home @12:25:49.58 Archive.seek :home @12:25:49.68 Air.seek :home @12:25:50.06 Lysis2.in :default @12:25:50.08 Toroid.setRawPosition! 0 @12:25:50.25 Toroid.seek :home @12:25:50.45 Air.in :default @12:25:50.49 Lysis1.in :default @12:25:50.52 Archive.in :default @12:25:50.57 Toroid.in :default @12:25:51.09 Toroid.seek :clear @12:25:52.82 Carousel.in :default @12:25:52.97 Carousel.home.seek 30 @12:25:53.32 Selecting Cartridge 28 @12:25:53.64 Carousel.seek 28 @12:25:54.40 Lysis1.in :fast @12:25:54.49 Lysis1.seek :locked @12:25:57.14 Plunge.seek :locked @12:25:57.33 Air.in :fast @12:25:57.42 Air.seek :locked @12:25:58.42 Carousel.reconfigure :locked @12:25:58.60 Powering cartridge bus @12:26:01.26 Gate.setIR 1750,5000,0,6 @12:26:01.39 Gate.setIR 0,5000,0,6 @12:26:01.47 PuckHeater.configure :default #ambient 24.4C @12:26:01.56 Gate.power :heat,:ON,limit to 5000mA @12:26:02.27 Mounted lysis Cartridge board#Q05045 @12:26:02.33 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:26:02.40 GoodNews.email "PhytoSampler filtering up to 175 ml",Subject: "Sampling microSPR_phyto cartridge in slot 28" @12:26:02.78 Preparing to filter cartridge 28 @12:26:03.07 Air.in :default @12:26:03.19 Air.seek :sealed @12:26:03.38 Priming sample loop w/150ml, bypass w/50ml @12:26:03.42 SamplePump.setPosition! 0ml @12:26:03.51 Bypass.open @12:26:05.02 Intake.open @12:26:05.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:26:08.44 Vacuum tare is 52% of full scale at 22C (drifted 1%) @12:26:08.52 upsync @12:26:08.70 Abs.in :default @12:26:08.80 Abs.hold @12:26:09.09 SamplePump.in :start @12:26:09.19 SamplePump.seek 50ml,3:50 @12:26:09.78 SamplePump.configure :prime @12:27:10.59 Abs.stop @12:27:10.77 SamplePump.setPosition! 0ml @12:27:10.85 Exhaust.open @12:27:12.25 Bypass.close @12:27:13.82 Abs.in :default @12:27:13.90 Abs.hold @12:27:14.24 SamplePump.in :start @12:27:14.34 SamplePump.seek 150ml,10:30 @12:27:14.93 SamplePump.configure :prime @12:30:17.85 Abs.stop @12:30:17.95 Bypass.open @12:30:18.67 Intake.close @12:30:19.53 Toroid.in :hold @12:30:19.62 Toroid.seek :crack @12:30:21.20 Toroid.seek :cartridge @12:30:22.34 Toroid.in :default @12:30:22.44 Toroid.seek :clear @12:30:24.50 Pressurizing cartridge to 26psi above 14.9psia @12:30:24.53 SamplePump.setPosition! 0ml @12:30:24.63 Intake.open @12:30:25.35 Bypass.close @12:30:26.08 Exhaust.close @12:30:26.90 Abs.in :default @12:30:27.45 Abs.hold @12:30:27.80 SamplePump.in :charge @12:30:27.89 SamplePump.seek 2.3ml,42 seconds @12:30:42.82 Abs.stop @12:30:42.92 Intake.close @12:30:43.70 Pressurized cartridge with 1.001ml to 26.5psi @12:30:43.72 Isolated @35.5psia (20.7psi) @12:30:43.75 Verifying cartridge holds at least 0.65..0.85 of 35.5psia @12:30:43.82 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:30:48.43 Cartridge pressure fell 2% to 34.8psia @12:30:48.54 Sampling 175.0ml @12:30:48.56 SamplePump.setPosition! 0ml @12:30:48.65 Exhaust.open @12:30:49.33 Intake.open @12:30:50.24 Abs.in :default @12:30:50.33 Abs.hold @12:30:50.67 SamplePump.in :start @12:30:50.80 SamplePump.seek 175ml,15:05 @12:30:51.36 SamplePump.configure :sample @12:30:53.76 Cartridge 28 filtering 2/175ml at 0.78ml/s with 21.2psi vacuum @12:31:53.82 Cartridge 28 filtering 39/175ml at 0.62ml/s with 15.6psi vacuum 8.5m deep (27.3psia) @12:32:53.82 Cartridge 28 filtering 74/175ml at 0.58ml/s with 27.5psi vacuum @12:33:53.82 Cartridge 28 filtering 107/175ml at 0.55ml/s with 26.2psi vacuum @12:34:53.82 Cartridge 28 filtering 137/175ml at 0.51ml/s with 18.5psi vacuum 5.2m deep (22.4psia) @12:35:53.82 Cartridge 28 filtering 165/175ml at 0.47ml/s with 29.9psi vacuum @12:36:16.10 Abs.stop @12:36:16.26 Intake.close @12:36:16.32 Exhaust.close @12:36:16.58 Toroid.in :hold @12:36:16.67 Toroid.seek :bypass @12:36:18.37 Toroid.in :default @12:36:18.45 Toroid.seek :clear @12:36:20.10 Sampled 175.0ml @12:36:21.09 GoodNews.email "After filtering 175.0ml",Subject: "Processing microSPR_phyto cartridge in slot 28" @12:36:22.79 SPR.command "read_sequences" @12:36:23.43 SPR.command "stats_on" @12:36:23.71 SPR.command "start 0 1" @12:36:24.36 SPR.start /var/log/esp/spr24aug2012h.spr.bz2 @12:36:26.29 Handoff.select :waste @12:36:26.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:36:26.53 upsync @12:36:33.13 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:36:33.17 PinPort.in :locking @12:36:33.26 PinPort.seek :locked @12:36:33.79 Archive.in :fast @12:36:33.92 Lysis2.in :fast @12:36:33.98 Lysis1.seek 1350uL @12:36:34.04 Archive.seek :full @12:36:34.22 Lysis2.seek 950uL @12:36:39.08 Plunge.seek :reagent @12:36:40.11 Evacuating puck with filter bubble point of 24psi @12:36:40.14 PinPort.seek :locked @12:36:40.29 Handoff.select :waste @12:36:40.34 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:36:46.94 Air syringe pumping up to 1 strokes to reach 8psi @12:36:46.95 Air stroke #1 of 1 @12:36:47.09 Air.in :pressurize @12:36:47.18 Air.seek :pressurized @12:36:52.83 1.6psi after pressurize @12:36:52.86 Air.in :compress @12:36:52.97 Air.seek :evac @12:36:56.02 2.0psi after compress @12:37:14.13 0.4psi after compress (stable) @12:37:14.62 Air.in :default @12:37:14.81 Air.hold @12:37:15.11 at 0.4psi Opened to waste @12:37:15.12 Handoff.select :blocked @12:37:15.30 Archive syringe pumping up to 8 strokes to reach 24psi @12:37:15.31 Archive stroke #1 of 8 @12:37:15.45 Archive.in :pressurize @12:37:15.54 Archive.seek :pressurized @12:37:28.00 2.5psi after pressurize @12:37:33.59 2.0psi after pressurize (stable) @12:37:33.61 Archive.in :compress @12:37:33.71 Archive.seek :empty @12:37:49.46 13.8psi after compress @12:38:00.05 14.1psi after compress (stable) @12:38:00.08 Archive.in :gulp @12:38:00.24 Archive.seek :topVent @12:38:06.58 15.4psi after gulp @12:38:12.18 14.6psi after gulp (stable) @12:38:12.19 Archive stroke #2 of 8 @12:38:12.33 Archive.in :pressurize @12:38:12.40 Archive.seek :pressurized @12:38:24.88 15.0psi after pressurize @12:38:30.48 14.3psi after pressurize (stable) @12:38:30.51 Archive.in :compress @12:38:30.62 Archive.seek :empty @12:38:42.79 Reached 24.1 of 24.0psi after pumping 3381uL (1.95 strokes) @12:38:42.81 Archive.in :default @12:38:42.93 Archive.hold @12:38:43.07 Handoff.select :waste @12:38:43.24 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:39:02.84 delay 1:00 @12:40:02.87 Handoff.select :blocked @12:40:03.08 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:40:25.68 Handoff.select :waste @12:40:25.84 0.4psi at evacEnd @12:40:25.85 Handoff.select :blocked @12:40:26.05 Archive.in :fast @12:40:26.16 Archive.seek :topVent @12:40:33.08 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @12:40:39.67 Stable @0.4psi after Handoff blocked @12:40:39.71 Archive syringe pumping up to 8 strokes to reach 24psi @12:40:39.74 Archive stroke #1 of 8 @12:40:39.88 Archive.in :pressurize @12:40:39.96 Archive.seek :pressurized @12:40:52.42 1.0psi after pressurize @12:40:52.44 Archive.in :compress @12:40:52.55 Archive.seek :empty @12:41:08.31 13.1psi after compress @12:41:18.90 12.9psi after compress (stable) @12:41:18.93 Archive.in :gulp @12:41:19.08 Archive.seek :topVent @12:41:25.41 13.9psi after gulp @12:41:31.02 13.1psi after gulp (stable) @12:41:31.03 Archive stroke #2 of 8 @12:41:31.18 Archive.in :pressurize @12:41:31.26 Archive.seek :pressurized @12:41:43.77 13.7psi after pressurize @12:41:43.80 Archive.in :compress @12:41:43.91 Archive.seek :empty @12:41:58.87 Reached 24.3 of 24.4psi after pumping 3441uL (1.99 strokes) @12:41:59.40 Archive.in :default @12:41:59.56 Archive.hold @12:41:59.71 Handoff.select :waste @12:41:59.88 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:42:06.98 delay 1:00 @12:43:07.00 Handoff.select :blocked @12:43:07.17 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @12:43:29.80 Handoff.select :waste @12:43:29.97 0.3psi at evacEnd @12:43:30.00 Air.seek :fullVent @12:43:31.36 Archive.in :fast @12:43:31.46 Archive.seek :topVent @12:43:38.59 Seawater evacuated, Pressure above/below filter released. @12:43:38.74 Lysis1.in :slow @12:43:38.83 Lysis1.seek 1050uL @12:44:08.49 delay 5 seconds @12:44:13.51 Handoff.select :blocked @12:44:13.75 Lysis1.seek 450uL @12:45:12.96 delay 3 seconds @12:45:16.05 Air.in mix with 1.57127 parts Lysis1 @12:45:16.85 Air.seek :evac @12:45:16.98 Lysis1.seek 300uL @12:45:31.91 Lysis1.seek :empty @12:46:01.63 Lysis buffer delivered @18.9psi @12:46:01.65 Polling Can every 2:00 @12:46:02.23 PuckHeater.configure :default #ambient 22.5C @12:46:02.31 PuckHeater.seek 79.7C @12:46:53.51 PuckHeater.hold 2:00,79.7C @12:48:53.15 Polling Can every 2:00 @12:48:53.71 PuckHeater.configure :default #ambient 22.6C @12:48:53.80 PuckHeater.hold 30 seconds,78.7C @12:49:23.89 Polling Can every 2:00 @12:49:24.44 PuckHeater.configure :default #ambient 22.6C @12:49:24.52 PuckHeater.hold 30 seconds,78.1C @12:49:54.62 Polling Can every 2:00 @12:49:55.18 PuckHeater.hold 10:00,77.6C @12:59:52.81 Lysis complete. Syringe pressure @23.4psi @12:59:52.84 Archive syringe pumping up to 5 strokes to reach 40psi @12:59:52.87 Archive stroke #1 of 5 @12:59:53.02 Archive.in :pressurize @12:59:53.10 Archive.seek :pressurized @13:00:05.61 23.5psi after pressurize @13:00:11.25 22.7psi after pressurize (stable) @13:00:11.28 Archive.in :compress @13:00:11.39 Archive.seek :empty @13:00:20.53 Reached 40.5 of 40.0psi after pumping 1588uL (0.92 strokes) @13:00:20.55 Archive.in :default @13:00:20.67 Archive.hold @13:00:20.81 delay 2 seconds @13:00:22.84 Archive.in :fast @13:00:23.44 Archive.seek :full @13:00:30.31 Lysis1.seek 138uL @13:00:44.04 delay 1 second @13:00:45.08 Archive.seek :full @13:00:45.18 Lysis1.seek :empty @13:00:58.98 Handoff.select :blocked @13:00:59.13 Initial air pressure @45.1psi @13:00:59.23 Lysis1.in :extract @13:00:59.34 Lysis1.seek 600uL,nil @13:01:03.05 Archive syringe pumping up to 6 strokes to reach 25psi @13:01:03.07 Archive stroke #1 of 6 @13:01:03.31 Archive.in :pressurize @13:01:03.40 Archive.seek :pressurized @13:01:15.86 12.6psi after pressurize @13:01:21.50 12.0psi after pressurize (stable) @13:01:21.53 Archive.in :compress @13:01:21.63 Archive.seek :empty @13:01:28.30 Archive.in :default @13:01:28.37 Archive.hold @13:01:28.58 Initial 600uL lysate @17.6psi @13:01:28.59 Handoff.select :blocked @13:01:28.77 Initial air pressure @16.9psi @13:01:28.91 Lysis1.seek 780uL,nil @13:01:30.24 Archive syringe pumping up to 6 strokes to reach 17psi @13:01:30.25 Archive stroke #1 of 6 @13:01:30.39 Archive.in :compress @13:01:30.47 Archive.seek :empty @13:01:31.25 Archive.in :default @13:01:31.32 Archive.hold @13:01:31.67 Recovered 780uL lysate @15.8psi @13:01:31.69 Air.in :halfSpeed @13:01:31.81 Air.seek :fullVent @13:01:33.85 Puck Top Vented, bottom @16.8psi @13:01:33.87 Handoff.select :waste @13:01:34.04 delay 5 seconds @13:01:39.14 Handoff open to waste, bottom @0.9psi @13:01:39.19 Air.in :default @13:01:39.29 Air.seek :evac @13:01:40.63 Lysis2.in :DAmix @13:01:40.82 Lysis1.in :DAmix @13:01:41.56 Lysis2.seek 800uL @13:01:41.65 Lysis1.seek 630uL @13:02:02.53 delay 5 seconds @13:02:07.55 Handoff.select SPR @13:02:07.71 SPR.command "start 2 14" @13:02:09.44 SPR stabilized @13:02:09.63 delay 10 seconds @13:02:19.65 SPR.command "start 2 0" @13:02:22.81 Waiting up to 11:00 for SPR to enter state 1 @13:04:20.90 SPR.command "hold_on" @13:04:22.31 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @13:04:23.06 Lysis2.seek 350uL @13:04:23.13 Lysis1.seek 180uL @13:05:25.36 delay 15 seconds @13:05:40.38 SPR.command "hold_off" @13:05:43.78 Waiting up to 1:00:00 for SPR to enter state 7 @13:21:59.34 SPRsummary:5.49RIU,52.3RIU,56RIU,4.06RIU,106ng/L,101ng/L,high after 2889s @13:36:16.24 SPR analysis completed @13:36:24.92 Ended log spr24aug2012h.spr.bz2 @13:36:24.94 SPR.command "stats_off" @13:36:25.37 Handoff.select :close @13:36:25.55 Gate.power :heat,:OFF @13:36:25.57 Gate.power :cartridge,:OFF @13:36:25.69 PinPort.in :unlocking @13:36:25.78 PinPort.seek :clear @13:36:26.29 Carousel.in :default @13:36:26.42 Carousel.home.seek 28 @13:36:26.81 Plunge.seek :locked @13:36:27.73 Archive.in :unlocking @13:36:27.87 Archive.seek :clear @13:36:28.51 Air.in :unlocking @13:36:28.67 Air.seek :clear @13:36:28.83 Lysis1.in :unlocking @13:36:28.98 Lysis1.seek :clear @13:36:29.12 Lysis2.in :unlocking @13:36:29.22 Lysis2.seek :clear @13:36:33.27 Gate.power :analytic1,:OFF @13:36:33.29 Gate.power :motor,:OFF @13:36:33.33 Gate.power :core,:OFF @13:36:33.38 Gate.power :main,:OFF @13:36:33.40 Safely stopped and ready to power off @13:37:24.36 -> PhytoSampler.goalVolume=75 75 @13:37:41.87 -> PhytoSampler.prime nil,nil PhytoSampler @13:37:52.69 -> PhytoSampler.xray @13:38:36.38 -> PhytoSampler.primeVolume=nil @13:38:43.17 -> PhytoSampler.xray @13:39:11.95 -> PhytoSampler.bypassVolume=nil @13:39:16.41 -> PhytoSampler.xray @13:41:02.02 -> fire lysis # @13:41:02.33 Gate.power :sampler,:OFF,limit to 3500mA @13:41:02.36 Gate.power :skimmer,:OFF,limit to 3500mA @13:41:02.40 Gate.power :main,:ON,limit to 5000mA @13:41:02.43 Gate.power :analytic1,:ON,limit to 4000mA @13:41:02.55 Powering core bus @13:41:04.89 SPR Module 1, FW Rev. B, FPGA Rev. C @13:41:05.33 Plunge.configure :default @13:41:05.44 SamplePump.configure :default @13:41:05.55 Toroid.configure :default @13:41:05.66 Carousel.configure :default @13:41:05.77 Abs.configure :default @13:41:05.89 PhaseSeparator.configure :default @13:41:05.99 Archive.configure :default @13:41:06.09 Air.configure :default @13:41:06.17 Lysis1.configure :default @13:41:06.24 Lysis2.configure :default @13:41:06.35 PinPort.configure :default @13:41:06.43 All elf controllers running firmware version 4.92 @13:41:06.46 Gate.power :motor,:ON,limit to 5000mA @13:41:06.90 Plunge.home.jog 0 @13:41:07.50 Toroid.home.jog 0 @13:41:07.57 PinPort.home.jog 0 @13:41:07.63 Lysis1.home.jog 0 @13:41:07.67 Air.home.jog 0 @13:41:07.73 Archive.home.jog 0 @13:41:07.79 Carousel.home.jog 0 @13:41:07.87 Lysis2.home.jog 0 @13:41:08.86 Plunge.in :homing @13:41:09.09 Air.in :homing @13:41:09.19 Plunge.jog 1604 @13:41:09.25 Lysis2.in :homing @13:41:09.30 Lysis1.in :homing @13:41:09.34 Toroid.in :homing @13:41:09.41 PinPort.in :homing @13:41:09.44 Archive.in :homing @13:41:09.83 Air.jog 1378 @13:41:09.88 Lysis2.jog 1406 @13:41:09.93 Lysis1.jog 1400 @13:41:10.53 PinPort.jog -110 @13:41:10.57 Toroid.jog 623 @13:41:10.65 Archive.jog 1402 @13:41:11.14 Plunge.setRawPosition! 50 @13:41:11.23 Plunge.seek :home @13:41:11.37 PinPort.setRawPosition! 0 @13:41:11.46 PinPort.seek :home @13:41:11.60 PinPort.in :default @13:41:11.68 Plunge.in :default @13:41:11.82 Air.setRawPosition! 50 @13:41:11.89 Air.seek :home @13:41:12.03 Lysis2.setRawPosition! 50 @13:41:12.15 Lysis2.seek :home @13:41:12.31 Lysis1.setRawPosition! 50 @13:41:12.33 Air.in :default @13:41:12.36 Archive.setRawPosition! 50 @13:41:12.55 Lysis1.seek :home @13:41:12.59 Archive.seek :home @13:41:12.85 Lysis2.in :default @13:41:12.99 Lysis1.in :default @13:41:13.13 Archive.in :default @13:41:13.15 Toroid.setRawPosition! 0 @13:41:13.30 Toroid.seek :home @13:41:13.41 Toroid.in :default @13:41:13.55 Toroid.seek :clear @13:41:15.20 Carousel.in :default @13:41:15.35 Carousel.home.seek 28 @13:41:15.64 Selecting Cartridge 26 @13:41:15.96 Carousel.seek 26 @13:41:16.76 Lysis1.in :fast @13:41:16.85 Lysis1.seek :locked @13:41:19.50 Plunge.seek :locked @13:41:19.69 Air.in :fast @13:41:19.77 Air.seek :locked @13:41:20.77 Carousel.reconfigure :locked @13:41:20.96 Powering cartridge bus @13:41:23.62 Gate.setIR 1750,5000,0,6 @13:41:23.75 Gate.setIR 0,5000,0,6 @13:41:23.84 PuckHeater.configure :default #ambient 22.6C @13:41:23.93 Gate.power :heat,:ON,limit to 5000mA @13:41:24.64 Mounted lysis Cartridge board#Q05057 @13:41:24.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:41:24.81 GoodNews.email "PhytoSampler filtering up to 75 ml",Subject: "Sampling microSPR_phyto cartridge in slot 26" @13:41:25.20 Preparing to filter cartridge 26 @13:41:26.04 Air.in :default @13:41:26.16 Bypass.open @13:41:26.22 Air.seek :sealed @13:41:27.13 Toroid.in :hold @13:41:27.21 Toroid.seek :crack @13:41:28.85 Toroid.seek :cartridge @13:41:30.08 Toroid.in :default @13:41:30.20 Toroid.seek :clear @13:41:32.04 Exhaust.open @13:41:32.88 Intake.open @13:41:33.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:41:35.54 upsync @13:41:36.30 Vacuum tare is 52% of full scale at 23C (drifted 1%) @13:41:36.49 Pressurizing cartridge to 26psi above 14.7psia @13:41:36.51 SamplePump.setPosition! 0ml @13:41:36.61 Bypass.close @13:41:37.28 Exhaust.close @13:41:38.06 Abs.in :default @13:41:38.15 Abs.hold @13:41:38.39 SamplePump.in :charge @13:41:38.48 SamplePump.seek 2.3ml,42 seconds @13:41:51.90 Abs.stop @13:41:52.01 Intake.close @13:41:52.82 Pressurized cartridge with 0.806ml to 26.6psi @13:41:52.83 Isolated @35.4psia (20.7psi) @13:41:52.87 Verifying cartridge holds at least 0.65..0.85 of 35.4psia @13:41:52.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:41:57.54 Cartridge pressure fell 1% to 35.0psia @13:41:57.67 Sampling 75.0ml @13:41:57.69 SamplePump.setPosition! 0ml @13:41:57.78 Exhaust.open @13:41:58.43 Intake.open @13:41:59.43 Abs.in :default @13:41:59.51 Abs.hold @13:41:59.87 SamplePump.in :start @13:41:59.97 SamplePump.seek 75ml,6:45 @13:42:00.56 SamplePump.configure :sample @13:42:02.98 Cartridge 26 filtering 2/75ml at 0.57ml/s with 21.5psi vacuum @13:43:03.04 Cartridge 26 filtering 39/75ml at 0.62ml/s with 11.8psi vacuum 7.4m deep (25.7psia) @13:44:03.03 Cartridge 26 filtering 73/75ml at 0.57ml/s with 25.9psi vacuum @13:44:06.18 Abs.stop @13:44:06.36 Intake.close @13:44:06.44 Exhaust.close @13:44:06.69 Toroid.in :hold @13:44:06.85 Toroid.seek :bypass @13:44:08.57 Toroid.in :default @13:44:08.66 Toroid.seek :clear @13:44:10.31 Sampled 75.0ml @13:44:10.81 GoodNews.email "After filtering 75.0ml",Subject: "Processing microSPR_phyto cartridge in slot 26" @13:44:12.44 SPR.command "read_sequences" @13:44:13.01 SPR.command "stats_on" @13:44:13.19 SPR.command "start 0 1" @13:44:13.90 SPR.start /var/log/esp/spr24aug2013h.spr.bz2 @13:44:15.87 Handoff.select :waste @13:44:15.99 upsync @13:44:16.08 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:44:22.70 Syringe tare is 51% of full scale at 23C (drifted 0%) @13:44:22.78 PinPort.in :locking @13:44:22.88 PinPort.seek :locked @13:44:23.40 Archive.in :fast @13:44:23.53 Lysis2.in :fast @13:44:23.60 Lysis1.seek 1350uL @13:44:23.64 Archive.seek :full @13:44:23.86 Lysis2.seek 950uL @13:44:28.70 Plunge.seek :reagent @13:44:29.71 Evacuating puck with filter bubble point of 24psi @13:44:29.74 PinPort.seek :locked @13:44:29.88 Handoff.select :waste @13:44:29.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:44:36.53 Air syringe pumping up to 1 strokes to reach 8psi @13:44:36.54 Air stroke #1 of 1 @13:44:36.68 Air.in :pressurize @13:44:36.80 Air.seek :pressurized @13:44:42.44 1.4psi after pressurize @13:44:42.47 Air.in :compress @13:44:42.58 Air.seek :evac @13:44:45.62 2.2psi after compress @13:44:56.23 0.1psi after compress (stable) @13:44:56.25 Air.in :default @13:44:56.35 Air.hold @13:44:56.59 at 0.0psi Opened to waste @13:44:56.61 Handoff.select :blocked @13:44:56.81 Archive syringe pumping up to 8 strokes to reach 24psi @13:44:56.82 Archive stroke #1 of 8 @13:44:56.95 Archive.in :pressurize @13:44:57.05 Archive.seek :pressurized @13:45:09.51 3.3psi after pressurize @13:45:23.60 9.0psi after pressurize (stable) @13:45:23.64 Archive.in :compress @13:45:23.74 Archive.seek :empty @13:45:39.49 17.3psi after compress @13:45:50.08 17.2psi after compress (stable) @13:45:50.11 Archive.in :gulp @13:45:50.22 Archive.seek :topVent @13:45:56.56 17.9psi after gulp @13:46:02.18 17.4psi after gulp (stable) @13:46:02.20 Archive stroke #2 of 8 @13:46:02.83 Archive.in :pressurize @13:46:03.01 Archive.seek :pressurized @13:46:15.49 17.7psi after pressurize @13:46:28.60 22.9psi after pressurize (stable) @13:46:28.63 Archive.in :compress @13:46:28.80 Archive.seek :empty @13:46:29.18 Reached 24.0 of 24.0psi after pumping 3131uL (1.81 strokes) @13:46:29.20 Archive.in :default @13:46:29.30 Archive.hold @13:46:29.41 Handoff.select :waste @13:46:29.56 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:46:46.66 delay 1:00 @13:47:46.68 Handoff.select :blocked @13:47:46.88 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @13:48:09.47 Handoff.select :waste @13:48:09.63 0.4psi at evacEnd @13:48:09.64 Handoff.select :blocked @13:48:09.82 Archive.in :fast @13:48:09.89 Archive.seek :topVent @13:48:15.96 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @13:48:22.55 Stable @0.3psi after Handoff blocked @13:48:22.58 Archive syringe pumping up to 8 strokes to reach 24psi @13:48:22.60 Archive stroke #1 of 8 @13:48:22.78 Archive.in :pressurize @13:48:22.87 Archive.seek :pressurized @13:48:35.32 3.1psi after pressurize @13:48:35.34 Archive.in :compress @13:48:35.46 Archive.seek :empty @13:48:51.25 15.3psi after compress @13:49:02.33 14.6psi after compress (stable) @13:49:02.47 Archive.in :gulp @13:49:02.58 Archive.seek :topVent @13:49:08.92 15.4psi after gulp @13:49:15.03 14.7psi after gulp (stable) @13:49:15.04 Archive stroke #2 of 8 @13:49:15.18 Archive.in :pressurize @13:49:15.29 Archive.seek :pressurized @13:49:27.73 15.4psi after pressurize @13:49:27.76 Archive.in :compress @13:49:27.86 Archive.seek :empty @13:49:36.87 Reached 24.5 of 24.3psi after pumping 3315uL (1.92 strokes) @13:49:36.89 Archive.in :default @13:49:37.00 Archive.hold @13:49:37.14 Handoff.select :waste @13:49:37.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:49:44.39 delay 1:00 @13:50:44.41 Handoff.select :blocked @13:50:44.59 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @13:51:07.18 Handoff.select :waste @13:51:07.33 0.3psi at evacEnd @13:51:07.37 Air.seek :fullVent @13:51:08.79 Archive.in :fast @13:51:08.89 Archive.seek :topVent @13:51:15.56 Seawater evacuated, Pressure above/below filter released. @13:51:15.70 Lysis1.in :slow @13:51:15.78 Lysis1.seek 1050uL @13:51:45.43 delay 5 seconds @13:51:50.45 Handoff.select :blocked @13:51:50.71 Lysis1.seek 450uL @13:52:49.95 delay 3 seconds @13:52:53.07 Air.in mix with 1.57127 parts Lysis1 @13:52:53.81 Air.seek :evac @13:52:53.92 Lysis1.seek 300uL @13:53:08.87 Lysis1.seek :empty @13:53:38.63 Lysis buffer delivered @43.0psi @13:53:38.65 Polling Can every 2:00 @13:53:39.22 PuckHeater.configure :default #ambient 23.3C @13:53:39.30 PuckHeater.seek 79.7C @13:54:37.95 PuckHeater.hold 2:00,79.7C @13:56:38.06 Polling Can every 2:00 @13:56:38.61 PuckHeater.configure :default #ambient 23.4C @13:56:38.70 PuckHeater.hold 30 seconds,78.6C @13:57:08.98 Polling Can every 2:00 @13:57:09.54 PuckHeater.configure :default #ambient 23.3C @13:57:09.62 PuckHeater.hold 30 seconds,78.1C @13:57:39.85 Polling Can every 2:00 @13:57:40.40 PuckHeater.configure :default #ambient 23.4C @13:57:40.48 PuckHeater.hold 10:00,77.6C @14:07:40.42 Lysis complete. Syringe pressure @47.3psi @14:07:40.45 Archive syringe pumping up to 5 strokes to reach 40psi @14:07:40.47 Archive stroke #1 of 5 @14:07:40.62 Archive.in :pressurize @14:07:40.70 Archive.seek :pressurized @14:07:41.01 Reached 47.9 of 40.0psi after pumping 0uL (0.00 strokes) @14:07:41.03 Archive.in :default @14:07:41.24 Archive.hold @14:07:41.35 delay 2 seconds @14:07:43.38 Archive.in :fast @14:07:43.46 Archive.seek :full @14:07:43.73 Lysis1.seek 138uL @14:07:57.50 delay 1 second @14:07:58.53 Archive.seek :full @14:07:58.61 Lysis1.seek :empty @14:08:12.45 Handoff.select :blocked @14:08:12.59 Initial air pressure @47.9psi @14:08:12.69 Lysis1.in :extract @14:08:12.83 Lysis1.seek 600uL,nil @14:08:15.33 Archive syringe pumping up to 6 strokes to reach 25psi @14:08:15.35 Archive stroke #1 of 6 @14:08:15.49 Archive.in :pressurize @14:08:15.59 Archive.seek :pressurized @14:08:18.98 Archive.in :default @14:08:19.07 Archive.hold @14:08:19.44 Initial 600uL lysate @12.5psi @14:08:19.46 Handoff.select :blocked @14:08:19.60 Initial air pressure @12.0psi @14:08:19.72 Lysis1.seek 780uL,nil @14:08:19.87 Archive syringe pumping up to 6 strokes to reach 17psi @14:08:19.89 Archive stroke #1 of 6 @14:08:20.09 Archive.in :pressurize @14:08:20.20 Archive.seek :pressurized @14:08:29.34 11.9psi after pressurize @14:08:45.44 15.0psi after pressurize (stable) @14:08:45.48 Archive.in :compress @14:08:45.59 Archive.seek :empty @14:08:47.30 Archive.in :default @14:08:47.37 Archive.hold @14:08:47.70 Recovered 780uL lysate @15.7psi @14:08:47.73 Air.in :halfSpeed @14:08:47.82 Air.seek :fullVent @14:08:49.86 Puck Top Vented, bottom @15.5psi @14:08:49.87 Handoff.select :waste @14:08:50.07 delay 5 seconds @14:08:55.15 Handoff open to waste, bottom @-0.1psi @14:08:55.18 Air.in :default @14:08:55.28 Air.seek :evac @14:08:56.63 Lysis2.in :DAmix @14:08:56.81 Lysis1.in :DAmix @14:08:57.58 Lysis2.seek 800uL @14:08:57.62 Lysis1.seek 630uL @14:09:18.56 delay 5 seconds @14:09:23.58 Handoff.select SPR @14:09:23.75 SPR.command "start 2 14" @14:09:24.47 SPR stabilized @14:09:24.66 delay 10 seconds @14:09:34.68 SPR.command "start 2 0" @14:09:37.84 Waiting up to 11:00 for SPR to enter state 1 @14:11:35.51 SPR.command "hold_on" @14:11:36.89 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @14:11:37.64 Lysis2.seek 350uL @14:11:37.74 Lysis1.seek 180uL @14:12:39.94 delay 15 seconds @14:12:54.96 SPR.command "hold_off" @14:12:58.35 Waiting up to 1:00:00 for SPR to enter state 7 @14:29:13.92 SPRsummary:-10.1RIU,39.9RIU,57.1RIU,-4.48RIU,231ng/L,159ng/L,high after 2852s @14:43:30.81 SPR analysis completed @14:43:39.56 Ended log spr24aug2013h.spr.bz2 @14:43:39.58 SPR.command "stats_off" @14:43:39.93 Handoff.select :close @14:43:40.10 Gate.power :heat,:OFF @14:43:40.14 Gate.power :cartridge,:OFF @14:43:40.25 PinPort.in :unlocking @14:43:40.33 PinPort.seek :clear @14:43:40.84 Carousel.in :default @14:43:40.98 Carousel.home.seek 26 @14:43:41.32 Plunge.seek :locked @14:43:42.76 Archive.in :unlocking @14:43:42.93 Archive.seek :clear @14:43:43.02 Air.in :unlocking @14:43:43.22 Air.seek :clear @14:43:43.37 Lysis1.in :unlocking @14:43:43.51 Lysis1.seek :clear @14:43:43.66 Lysis2.in :unlocking @14:43:43.74 Lysis2.seek :clear @14:43:47.81 Gate.power :analytic1,:OFF @14:43:47.83 Gate.power :motor,:OFF @14:43:47.90 Gate.power :core,:OFF @14:43:47.92 Gate.power :main,:OFF @14:43:47.95 Safely stopped and ready to power off @14:44:15.32 -> BACsampler.xray @14:44:35.70 -> BACsampler.bypassVolume=nil @14:44:48.02 -> BACsampler.primeVolume=nil @14:45:04.02 -> BACsampler.goalVolume=175 175 @14:45:18.14 -> BACsamplerxray @14:45:18.17 NameError in bill -- uninitialized constant BACsamplerxray @14:45:27.78 -> BACsampler.xray @14:46:15.58 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [45,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75.0, lysed: 780, spr: spr24aug2013h.spr.bz2}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175.0, lysed: 780, spr: spr24aug2012h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: spr24aug1919h.spr.bz2}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100.0, lysed: 780, spr: spr24aug1917h.spr.bz2}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, [58,56] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @14:48:00.97 -> fire archive @14:48:01.19 Gate.power :sampler,:OFF,limit to 3500mA # @14:48:01.24 Gate.power :skimmer,:OFF,limit to 3500mA @14:48:01.30 Gate.power :main,:ON,limit to 5000mA @14:48:01.32 Gate.power :analytic1,:ON,limit to 4000mA @14:48:01.43 Powering core bus @14:48:03.77 SPR Module 1, FW Rev. B, FPGA Rev. C @14:48:04.22 Plunge.configure :default @14:48:04.32 SamplePump.configure :default @14:48:04.43 Toroid.configure :default @14:48:04.54 Carousel.configure :default @14:48:04.65 Abs.configure :default @14:48:04.78 PhaseSeparator.configure :default @14:48:04.89 Archive.configure :default @14:48:05.00 Air.configure :default @14:48:05.07 Lysis1.configure :default @14:48:05.15 Lysis2.configure :default @14:48:05.26 PinPort.configure :default @14:48:05.34 All elf controllers running firmware version 4.92 @14:48:05.38 Gate.power :motor,:ON,limit to 5000mA @14:48:05.80 Plunge.home.jog 0 @14:48:06.43 Lysis2.home.jog 0 @14:48:06.49 Air.home.jog 0 @14:48:06.54 Carousel.home.jog 0 @14:48:06.60 Toroid.home.jog 0 @14:48:06.65 Archive.home.jog 0 @14:48:06.68 PinPort.home.jog 0 @14:48:06.76 Lysis1.home.jog 0 @14:48:07.62 Plunge.in :homing @14:48:07.98 Lysis1.in :homing @14:48:08.09 Plunge.jog 1604 @14:48:08.60 Lysis2.in :homing @14:48:08.77 Air.in :homing @14:48:08.83 Toroid.in :homing @14:48:08.87 PinPort.in :homing @14:48:08.92 Archive.in :homing @14:48:09.25 Lysis1.jog 1397 @14:48:09.29 Air.jog 1382 @14:48:09.36 Lysis2.jog 1404 @14:48:09.50 Toroid.jog 624 @14:48:09.76 Archive.jog 1402 @14:48:09.80 PinPort.jog -113 @14:48:10.24 PinPort.setRawPosition! 0 @14:48:10.34 PinPort.seek :home @14:48:10.47 Plunge.setRawPosition! 50 @14:48:10.50 PinPort.in :default @14:48:10.64 Plunge.seek :home @14:48:10.84 Lysis1.setRawPosition! 50 @14:48:10.89 Air.setRawPosition! 50 @14:48:11.02 Lysis1.seek :home @14:48:11.07 Air.seek :home @14:48:11.30 Lysis2.setRawPosition! 50 @14:48:11.34 Archive.setRawPosition! 50 @14:48:11.37 Plunge.in :default @14:48:11.59 Archive.seek :home @14:48:11.64 Lysis2.seek :home @14:48:11.93 Air.in :default @14:48:11.95 Toroid.setRawPosition! 0 @14:48:12.02 Lysis1.in :default @14:48:12.21 Toroid.seek :home @14:48:12.35 Archive.in :default @14:48:12.40 Lysis2.in :default @14:48:12.59 Toroid.in :default @14:48:12.82 Toroid.seek :clear @14:48:14.56 Carousel.in :default @14:48:14.70 Carousel.home.seek 26 @14:48:15.04 Selecting Cartridge 45 @14:48:15.37 Carousel.seek 45 @14:48:19.60 Lysis1.in :fast @14:48:19.69 Lysis1.seek :locked @14:48:22.34 Plunge.seek :locked @14:48:22.52 Air.in :fast @14:48:22.60 Air.seek :locked @14:48:23.60 Carousel.reconfigure :locked @14:48:23.78 Powering cartridge bus @14:48:26.45 Gate.setIR 1750,5000,0,6 @14:48:26.58 Gate.setIR 0,5000,0,6 @14:48:26.68 Gate.power :cartridge,:OFF @14:48:27.26 Mounted archive Cartridge @14:48:27.37 GoodNews.email "BACsampler filtering up to 175 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 45" @14:48:28.25 Preparing to filter cartridge 45 @14:48:28.57 Bypass.open @14:48:29.44 Toroid.in :hold @14:48:29.53 Toroid.seek :crack @14:48:31.13 Toroid.seek :cartridge @14:48:32.30 Toroid.in :default @14:48:32.40 Toroid.seek :clear @14:48:34.23 Exhaust.open @14:48:34.99 Intake.open @14:48:35.80 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:48:38.41 upsync @14:48:38.50 Vacuum tare is 52% of full scale at 23C (drifted 1%) @14:48:38.70 Pressurizing cartridge to 26psi above 14.8psia @14:48:38.76 SamplePump.setPosition! 0ml @14:48:38.85 Bypass.close @14:48:39.52 Exhaust.close @14:48:40.33 Abs.in :default @14:48:40.41 Abs.hold @14:48:40.67 SamplePump.in :charge @14:48:40.82 SamplePump.seek 2.3ml,42 seconds @14:48:52.88 Abs.stop @14:48:52.98 Intake.close @14:48:53.73 Pressurized cartridge with 0.651ml to 26.1psi @14:48:53.75 Isolated @37.9psia (23.1psi) @14:48:53.78 Verifying cartridge holds at least 0.65..0.85 of 37.9psia @14:48:53.84 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:48:58.45 Cartridge pressure fell 1% to 37.4psia @14:48:58.57 Sampling 175.0ml @14:48:58.59 SamplePump.setPosition! 0ml @14:48:58.68 Exhaust.open @14:48:59.40 Intake.open @14:49:00.39 Abs.in :default @14:49:00.47 Abs.hold @14:49:00.87 SamplePump.in :start @14:49:00.97 SamplePump.seek 175ml,15:05 @14:49:01.56 SamplePump.configure :sample @14:49:03.97 Cartridge 45 filtering 2/175ml at 0.63ml/s with 18.6psi vacuum 4.1m deep (20.8psia) @14:50:04.03 Cartridge 45 filtering 36/175ml at 0.57ml/s with 23.6psi vacuum @14:51:04.03 Cartridge 45 filtering 67/175ml at 0.52ml/s with 28.4psi vacuum @14:52:04.02 Cartridge 45 filtering 96/175ml at 0.48ml/s with 24.6psi vacuum @14:53:04.03 Cartridge 45 filtering 123/175ml at 0.44ml/s with 21.0psi vacuum @14:54:04.02 Cartridge 45 filtering 146/175ml at 0.39ml/s with 21.2psi vacuum @14:55:04.02 Cartridge 45 filtering 166/175ml at 0.33ml/s with 29.3psi vacuum @14:55:36.87 Abs.stop @14:55:37.04 Intake.close @14:55:37.12 Exhaust.close @14:55:37.36 Toroid.in :hold @14:55:37.45 Toroid.seek :bypass @14:55:39.11 Toroid.in :default @14:55:39.21 Toroid.seek :clear @14:55:40.85 Sampled 175.0ml @14:55:41.33 GoodNews.email "After filtering 175.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 45" @14:55:42.36 Plunge.seek :reagent @14:55:43.21 Sample loop closed. Ready to Deliver Reagents @14:55:43.24 Archive.seek :sealed @14:55:47.02 upsync @14:55:47.25 Archive.seek :evac @14:56:01.55 delay 1:00 @14:57:01.59 Archive.seek :clear @14:57:19.32 RNAlater soak for 10:00 @15:07:19.36 Archive.seek :tVent @15:07:22.00 delay 1 second @15:07:23.03 Archive.seek :evac @15:07:38.73 delay 10 seconds @15:07:48.78 Archive.seek :tVent @15:08:04.47 delay 1 second @15:08:05.50 Archive.seek :evac @15:08:21.18 delay 10 seconds @15:08:31.22 Sample preserved and evacuated @15:08:31.52 Carousel.in :default @15:08:31.65 Carousel.home.seek 45 @15:08:32.01 Plunge.seek :locked @15:08:32.93 Archive.in :unlocking @15:08:33.11 Archive.seek :clear @15:08:33.22 Air.in :unlocking @15:08:33.40 Air.seek :clear @15:08:33.54 Lysis1.in :unlocking @15:08:33.69 Lysis1.seek :clear @15:08:37.88 Gate.power :analytic1,:OFF @15:08:37.92 Gate.power :motor,:OFF @15:08:37.95 Gate.power :core,:OFF @15:08:37.98 Gate.power :main,:OFF @15:08:38.00 Safely stopped and ready to power off @15:09:31.00 -> fire archive # @15:09:31.27 Gate.power :sampler,:OFF,limit to 3500mA @15:09:31.29 Gate.power :skimmer,:OFF,limit to 3500mA @15:09:31.33 Gate.power :main,:ON,limit to 5000mA @15:09:31.36 Gate.power :analytic1,:ON,limit to 4000mA @15:09:31.48 Powering core bus @15:09:33.83 SPR Module 1, FW Rev. B, FPGA Rev. C @15:09:34.26 Plunge.configure :default @15:09:34.38 SamplePump.configure :default @15:09:34.48 Toroid.configure :default @15:09:34.58 Carousel.configure :default @15:09:34.69 Abs.configure :default @15:09:34.84 PhaseSeparator.configure :default @15:09:34.96 Archive.configure :default @15:09:35.06 Air.configure :default @15:09:35.13 Lysis1.configure :default @15:09:35.22 Lysis2.configure :default @15:09:35.33 PinPort.configure :default @15:09:35.42 All elf controllers running firmware version 4.92 @15:09:35.45 Gate.power :motor,:ON,limit to 5000mA @15:09:36.01 Plunge.home.jog 0 @15:09:36.12 Toroid.home.jog 0 @15:09:36.61 Lysis2.home.jog 0 @15:09:36.68 Lysis1.home.jog 0 @15:09:36.78 Archive.home.jog 0 @15:09:36.83 Carousel.home.jog 0 @15:09:36.90 PinPort.home.jog 0 @15:09:36.96 Air.home.jog 0 @15:09:38.28 Toroid.in :homing @15:09:38.54 Plunge.in :homing @15:09:38.74 Plunge.jog 1604 @15:09:38.78 Toroid.jog 631 @15:09:39.06 Lysis2.in :homing @15:09:39.12 Lysis1.in :homing @15:09:39.17 PinPort.in :homing @15:09:39.21 Archive.in :homing @15:09:39.25 Air.in :homing @15:09:39.53 Lysis2.jog 1402 @15:09:39.60 Lysis1.jog 1400 @15:09:39.63 PinPort.jog -112 @15:09:39.68 Archive.jog 1402 @15:09:39.77 Air.jog 1375 @15:09:40.16 Plunge.setRawPosition! 50 @15:09:40.24 Plunge.seek :home @15:09:40.37 PinPort.setRawPosition! 0 @15:09:40.45 PinPort.seek :home @15:09:40.60 PinPort.in :default @15:09:40.62 Plunge.in :default @15:09:40.93 Lysis2.setRawPosition! 50 @15:09:41.03 Lysis2.seek :home @15:09:41.20 Lysis1.setRawPosition! 50 @15:09:41.24 Archive.setRawPosition! 50 @15:09:41.29 Air.setRawPosition! 50 @15:09:41.37 Toroid.setRawPosition! 0 @15:09:41.57 Lysis1.seek :home @15:09:41.63 Archive.seek :home @15:09:41.68 Air.seek :home @15:09:41.78 Toroid.seek :home @15:09:42.16 Lysis1.in :default @15:09:42.20 Toroid.in :default @15:09:42.24 Lysis2.in :default @15:09:42.49 Air.in :default @15:09:42.51 Archive.in :default @15:09:42.92 Toroid.seek :clear @15:09:44.61 Carousel.in :default @15:09:44.80 Carousel.home.seek 45 @15:09:45.10 Selecting Cartridge 43 @15:09:45.42 Carousel.seek 43 @15:09:46.20 Lysis1.in :fast @15:09:46.27 Lysis1.seek :locked @15:09:48.94 Plunge.seek :locked @15:09:49.13 Air.in :fast @15:09:49.20 Air.seek :locked @15:09:50.20 Carousel.reconfigure :locked @15:09:50.78 Powering cartridge bus @15:09:53.04 Gate.setIR 1750,5000,0,6 @15:09:53.17 Gate.setIR 0,5000,0,6 @15:09:53.27 Gate.power :cartridge,:OFF @15:09:53.85 Mounted archive Cartridge @15:09:53.98 GoodNews.email "BACsampler filtering up to 175 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 43" @15:09:54.31 Preparing to filter cartridge 43 @15:09:54.58 Bypass.open @15:09:55.38 Toroid.in :hold @15:09:55.47 Toroid.seek :crack @15:09:57.04 Toroid.seek :cartridge @15:09:58.20 Toroid.in :default @15:09:58.29 Toroid.seek :clear @15:10:00.18 Exhaust.open @15:10:00.86 upsync @15:10:01.00 Intake.open @15:10:01.79 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:10:04.41 Vacuum tare is 52% of full scale at 23C (drifted 1%) @15:10:04.60 Pressurizing cartridge to 26psi above 15.0psia @15:10:04.63 SamplePump.setPosition! 0ml @15:10:04.74 Bypass.close @15:10:05.46 Exhaust.close @15:10:06.26 Abs.hold @15:10:06.52 SamplePump.in :charge @15:10:06.62 SamplePump.seek 2.3ml,42 seconds @15:10:17.45 Abs.stop @15:10:17.55 Intake.close @15:10:18.33 Pressurized cartridge with 0.521ml to 27.4psi @15:10:18.35 Isolated @35.6psia (20.7psi) @15:10:18.38 Verifying cartridge holds at least 0.65..0.85 of 35.6psia @15:10:18.45 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:10:23.06 Cartridge pressure fell 4% to 34.3psia @15:10:23.18 Sampling 175.0ml @15:10:23.20 SamplePump.setPosition! 0ml @15:10:23.29 Exhaust.open @15:10:23.95 Intake.open @15:10:24.90 Abs.in :default @15:10:24.97 Abs.hold @15:10:25.34 SamplePump.in :start @15:10:25.44 SamplePump.seek 175ml,15:05 @15:10:26.02 SamplePump.configure :sample @15:10:28.43 Cartridge 43 filtering 1/175ml at 0.59ml/s with 18.1psi vacuum 7.8m deep (26.2psia) @15:11:28.48 Cartridge 43 filtering 29/175ml at 0.46ml/s with 29.0psi vacuum @15:12:28.48 Cartridge 43 filtering 53/175ml at 0.41ml/s with 20.7psi vacuum @15:13:28.48 Cartridge 43 filtering 75/175ml at 0.37ml/s with 29.3psi vacuum @15:14:28.48 Cartridge 43 filtering 95/175ml at 0.33ml/s with 25.7psi vacuum @15:15:28.48 Cartridge 43 filtering 113/175ml at 0.30ml/s with 25.4psi vacuum @15:16:28.47 Cartridge 43 filtering 129/175ml at 0.26ml/s with 23.7psi vacuum @15:17:28.48 Cartridge 43 filtering 143/175ml at 0.24ml/s with 24.9psi vacuum @15:18:28.48 Cartridge 43 filtering 156/175ml at 0.21ml/s with 23.6psi vacuum @15:19:28.48 Cartridge 43 filtering 167/175ml at 0.18ml/s with 21.2psi vacuum @15:19:28.55 SamplePump.stop @15:19:28.64 Intake.close @15:19:28.68 Exhaust.close @15:19:29.01 Toroid.in :hold @15:19:29.10 Toroid.seek :bypass @15:19:30.81 Toroid.in :default @15:19:30.91 Toroid.seek :clear @15:19:32.55 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @15:19:32.57 Abs.stop @15:19:32.89 Sampled 166.8ml @15:19:33.33 GoodNews.email "After filtering 166.8ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 43" @15:19:33.94 Plunge.seek :reagent @15:19:35.33 Sample loop closed. Ready to Deliver Reagents @15:19:35.37 Archive.seek :sealed @15:19:39.40 Archive.seek :evac @15:19:39.68 upsync @15:19:53.72 delay 1:00 @15:20:53.76 Archive.seek :clear @15:21:11.51 RNAlater soak for 10:00 @15:31:11.55 Archive.seek :tVent @15:31:14.11 delay 1 second @15:31:15.14 Archive.seek :evac @15:31:30.83 delay 10 seconds @15:31:40.87 Archive.seek :tVent @15:31:56.57 delay 1 second @15:31:57.60 Archive.seek :evac @15:32:13.29 delay 10 seconds @15:32:23.32 Sample preserved and evacuated @15:32:23.62 Carousel.in :default @15:32:23.76 Carousel.home.seek 43 @15:32:24.13 Plunge.seek :locked @15:32:25.06 Archive.in :unlocking @15:32:25.23 Archive.seek :clear @15:32:25.30 Air.in :unlocking @15:32:25.49 Air.seek :clear @15:32:25.67 Lysis1.in :unlocking @15:32:25.82 Lysis1.seek :clear @15:32:29.98 Gate.power :analytic1,:OFF @15:32:30.00 Gate.power :motor,:OFF @15:32:30.05 Gate.power :core,:OFF @15:32:30.08 Gate.power :main,:OFF @15:32:30.11 Safely stopped and ready to power off @15:32:55.21 -> BACsampler.bypassVolume @15:33:05.14 -> BACsampler.xray @15:33:24.95 -> BACsampler.bypassVolume=50 50 @15:33:41.66 -> BACsampler.primeVolume=150 150 @15:34:00.56 -> BACsampler.goalVolume=175 175 @15:34:37.41 -> fire archive, archive, archive # @15:34:37.66 Gate.power :sampler,:OFF,limit to 3500mA @15:34:37.69 Gate.power :skimmer,:OFF,limit to 3500mA @15:34:37.74 Gate.power :main,:ON,limit to 5000mA @15:34:37.76 Gate.power :analytic1,:ON,limit to 4000mA @15:34:37.87 Powering core bus @15:34:40.22 SPR Module 1, FW Rev. B, FPGA Rev. C @15:34:40.65 Plunge.configure :default @15:34:40.78 SamplePump.configure :default @15:34:40.88 Toroid.configure :default @15:34:40.98 Carousel.configure :default @15:34:41.09 Abs.configure :default @15:34:41.20 PhaseSeparator.configure :default @15:34:41.31 Archive.configure :default @15:34:41.95 Air.configure :default @15:34:42.04 Lysis1.configure :default @15:34:42.11 Lysis2.configure :default @15:34:42.23 PinPort.configure :default @15:34:42.31 All elf controllers running firmware version 4.92 @15:34:42.33 Gate.power :motor,:ON,limit to 5000mA @15:34:42.87 Toroid.home.jog 0 @15:34:42.94 Plunge.home.jog 0 @15:34:43.60 PinPort.home.jog 0 @15:34:43.66 Lysis1.home.jog 0 @15:34:43.70 Archive.home.jog 0 @15:34:43.81 Air.home.jog 0 @15:34:43.85 Carousel.home.jog 0 @15:34:43.89 Lysis2.home.jog 0 @15:34:44.65 Plunge.in :homing @15:34:44.73 Toroid.in :homing @15:34:45.13 Toroid.jog 628 @15:34:45.21 Plunge.jog 1608 @15:34:45.25 Lysis2.in :homing @15:34:45.32 Air.in :homing @15:34:45.36 Lysis1.in :homing @15:34:45.40 Archive.in :homing @15:34:45.44 PinPort.in :homing @15:34:45.76 Lysis2.jog 1405 @15:34:45.85 Air.jog 1374 @15:34:45.94 PinPort.jog -112 @15:34:46.01 Archive.jog 1401 @15:34:46.08 Lysis1.jog 1397 @15:34:46.45 PinPort.setRawPosition! 0 @15:34:46.54 PinPort.seek :home @15:34:46.66 PinPort.in :default @15:34:46.81 Plunge.setRawPosition! 50 @15:34:46.88 Plunge.seek :home @15:34:47.15 Lysis2.setRawPosition! 50 @15:34:47.24 Lysis2.seek :home @15:34:47.38 Air.setRawPosition! 50 @15:34:47.41 Plunge.in :default @15:34:47.57 Air.seek :home @15:34:47.74 Lysis1.setRawPosition! 50 @15:34:47.78 Archive.setRawPosition! 50 @15:34:47.82 Lysis2.in :default @15:34:47.86 Toroid.setRawPosition! 0 @15:34:48.08 Lysis1.seek :home @15:34:48.15 Archive.seek :home @15:34:48.19 Toroid.seek :home @15:34:48.49 Toroid.in :default @15:34:48.51 Air.in :default @15:34:48.76 Lysis1.in :default @15:34:48.81 Archive.in :default @15:34:49.20 Toroid.seek :clear @15:34:50.94 Carousel.in :default @15:34:51.08 Carousel.home.seek 43 @15:34:51.90 Selecting Cartridge 41 @15:34:52.23 Carousel.seek 41 @15:34:52.99 Lysis1.in :fast @15:34:53.09 Lysis1.seek :locked @15:34:55.74 Plunge.seek :locked @15:34:55.93 Air.in :fast @15:34:56.01 Air.seek :locked @15:34:57.01 Carousel.reconfigure :locked @15:34:57.20 Powering cartridge bus @15:34:59.87 Gate.setIR 1750,5000,0,6 @15:35:00.00 Gate.setIR 0,5000,0,6 @15:35:00.10 Gate.power :cartridge,:OFF @15:35:00.67 Mounted archive Cartridge @15:35:00.79 GoodNews.email "BACsampler filtering up to 175 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 41" @15:35:01.16 Preparing to filter cartridge 41 @15:35:01.57 Priming sample loop w/150ml, bypass w/50ml @15:35:01.59 SamplePump.setPosition! 0ml @15:35:01.69 Bypass.open @15:35:03.23 Intake.open @15:35:03.98 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:35:06.60 Vacuum tare is 52% of full scale at 23C (drifted 1%) @15:35:06.85 Abs.in :default @15:35:06.94 Abs.hold @15:35:07.10 upsync @15:35:07.32 SamplePump.in :start @15:35:07.42 SamplePump.seek 50ml,3:50 @15:35:08.01 SamplePump.configure :prime @15:36:08.84 Abs.stop @15:36:08.93 SamplePump.setPosition! 0ml @15:36:09.59 Exhaust.open @15:36:10.95 Bypass.close @15:36:12.50 Abs.in :default @15:36:12.59 Abs.hold @15:36:12.97 SamplePump.in :start @15:36:13.06 SamplePump.seek 150ml,10:30 @15:36:13.66 SamplePump.configure :prime @15:39:16.53 Abs.stop @15:39:16.64 Bypass.open @15:39:17.34 Intake.close @15:39:18.15 Toroid.in :hold @15:39:18.23 Toroid.seek :crack @15:39:19.80 Toroid.seek :cartridge @15:39:20.94 Toroid.in :default @15:39:21.04 Toroid.seek :clear @15:39:23.09 Pressurizing cartridge to 26psi above 15.6psia @15:39:23.12 SamplePump.setPosition! 0ml @15:39:23.21 Intake.open @15:39:23.94 Bypass.close @15:39:24.61 Exhaust.close @15:39:25.41 Abs.in :default @15:39:25.49 Abs.hold @15:39:25.74 SamplePump.in :charge @15:39:25.83 SamplePump.seek 2.3ml,42 seconds @15:39:38.27 Abs.stop @15:39:38.37 Intake.close @15:39:39.16 Pressurized cartridge with 0.693ml to 26.4psi @15:39:39.18 Isolated @40.1psia (24.5psi) @15:39:39.20 Verifying cartridge holds at least 0.65..0.85 of 40.1psia @15:39:39.26 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:39:43.96 Sampling 175.0ml @15:39:43.99 SamplePump.setPosition! 0ml @15:39:44.09 Exhaust.open @15:39:44.77 Intake.open @15:39:45.62 Abs.in :default @15:39:45.71 Abs.hold @15:39:46.60 SamplePump.in :start @15:39:46.70 SamplePump.seek 175ml,15:05 @15:39:47.29 SamplePump.configure :sample @15:39:49.70 Cartridge 41 filtering 2/175ml at 0.70ml/s with 19.2psi vacuum @15:40:49.75 Cartridge 41 filtering 38/175ml at 0.60ml/s with 18.2psi vacuum @15:41:49.75 Cartridge 41 filtering 70/175ml at 0.54ml/s with 22.9psi vacuum @15:42:49.75 Cartridge 41 filtering 100/175ml at 0.50ml/s with 24.7psi vacuum @15:43:49.74 Cartridge 41 filtering 127/175ml at 0.45ml/s with 25.8psi vacuum @15:44:49.75 Cartridge 41 filtering 151/175ml at 0.40ml/s with 26.3psi vacuum @15:45:49.75 Cartridge 41 filtering 171/175ml at 0.34ml/s with 22.2psi vacuum @15:46:02.46 Abs.stop @15:46:02.63 Intake.close @15:46:02.67 Exhaust.close @15:46:02.98 Toroid.in :hold @15:46:03.06 Toroid.seek :bypass @15:46:04.74 Toroid.in :default @15:46:04.85 Toroid.seek :clear @15:46:06.50 Sampled 175.0ml @15:46:06.98 GoodNews.email "After filtering 175.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 41" @15:46:07.58 Plunge.seek :reagent @15:46:08.47 Sample loop closed. Ready to Deliver Reagents @15:46:08.51 Archive.seek :sealed @15:46:12.25 upsync @15:46:12.49 Archive.seek :evac @15:46:26.80 delay 1:00 @15:47:26.84 Archive.seek :clear @15:47:44.59 RNAlater soak for 10:00 @15:57:44.62 Archive.seek :tVent @15:57:47.18 delay 1 second @15:57:48.21 Archive.seek :evac @15:58:03.89 delay 10 seconds @15:58:14.41 Archive.seek :tVent @15:58:30.19 delay 1 second @15:58:31.22 Archive.seek :evac @15:58:46.91 delay 10 seconds @15:58:56.94 Sample preserved and evacuated @15:58:57.11 Selecting Cartridge 39 @15:58:57.37 Carousel.in :default @15:58:57.52 Carousel.home.seek 41 @15:58:57.91 Archive.in :unlocking @15:58:58.06 Archive.seek :clear @15:58:58.17 Air.in :unlocking @15:58:58.36 Air.seek :clear @15:59:02.67 Lysis1.in :unlocking @15:59:02.87 Lysis1.seek :clear @15:59:02.98 Plunge.in :unlocking @15:59:03.08 Plunge.seek :clear @15:59:04.51 Carousel.seek 39 @15:59:05.29 Lysis1.in :fast @15:59:05.37 Lysis1.seek :locked @15:59:08.03 Plunge.in :default @15:59:08.12 Plunge.seek :locked @15:59:08.32 Air.in :fast @15:59:08.41 Air.seek :locked @15:59:09.40 Carousel.reconfigure :locked @15:59:09.58 Powering cartridge bus @15:59:12.25 Gate.setIR 1750,5000,0,6 @15:59:12.38 Gate.setIR 0,5000,0,6 @15:59:12.48 Gate.power :cartridge,:OFF @15:59:13.05 Mounted archive Cartridge @15:59:13.15 GoodNews.email "BACsampler filtering up to 175 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 39" @15:59:13.49 Preparing to filter cartridge 39 @15:59:13.84 Priming sample loop w/150ml, bypass w/50ml @15:59:13.86 SamplePump.setPosition! 0ml @15:59:13.94 Bypass.open @15:59:15.40 Intake.open @15:59:16.40 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:59:19.03 Vacuum tare is 52% of full scale at 24C (drifted 1%) @15:59:19.24 upsync @15:59:19.29 Abs.in :default @15:59:19.38 Abs.hold @15:59:19.67 SamplePump.in :start @15:59:19.78 SamplePump.seek 50ml,3:50 @15:59:20.36 SamplePump.configure :prime @16:00:21.19 Abs.stop @16:00:21.28 SamplePump.setPosition! 0ml @16:00:21.35 Exhaust.open @16:00:22.76 Bypass.close @16:00:24.29 Abs.in :default @16:00:24.38 Abs.hold @16:00:24.74 SamplePump.in :start @16:00:24.87 SamplePump.seek 150ml,10:30 @16:00:25.41 SamplePump.configure :prime @16:03:28.38 Abs.stop @16:03:28.49 Bypass.open @16:03:29.20 Intake.close @16:03:30.00 Toroid.in :hold @16:03:30.10 Toroid.seek :crack @16:03:31.67 Toroid.seek :cartridge @16:03:32.81 Toroid.in :default @16:03:32.90 Toroid.seek :clear @16:03:35.00 Pressurizing cartridge to 26psi above 15.6psia @16:03:35.02 SamplePump.setPosition! 0ml @16:03:35.11 Intake.open @16:03:35.79 Bypass.close @16:03:36.49 Exhaust.close @16:03:37.28 Abs.in :default @16:03:37.35 Abs.hold @16:03:37.61 SamplePump.in :charge @16:03:37.71 SamplePump.seek 2.3ml,42 seconds @16:03:50.24 Abs.stop @16:03:50.34 Intake.close @16:03:51.11 Pressurized cartridge with 0.641ml to 26.0psi @16:03:51.12 Isolated @39.8psia (24.2psi) @16:03:51.16 Verifying cartridge holds at least 0.65..0.85 of 39.8psia @16:03:51.23 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:03:55.84 Cartridge pressure fell 1% to 39.6psia @16:03:55.96 Sampling 175.0ml @16:03:55.99 SamplePump.setPosition! 0ml @16:03:56.08 Exhaust.open @16:03:56.77 Intake.open @16:03:57.68 Abs.in :default @16:03:57.75 Abs.hold @16:03:58.10 SamplePump.in :start @16:03:58.20 SamplePump.seek 175ml,15:05 @16:03:58.79 SamplePump.configure :sample @16:04:01.19 Cartridge 39 filtering 2/175ml at 0.52ml/s with 23.0psi vacuum @16:05:01.25 Cartridge 39 filtering 38/175ml at 0.60ml/s with 21.3psi vacuum @16:06:01.25 Cartridge 39 filtering 71/175ml at 0.56ml/s with 25.3psi vacuum @16:07:01.24 Cartridge 39 filtering 103/175ml at 0.53ml/s with 18.4psi vacuum @16:08:01.25 Cartridge 39 filtering 132/175ml at 0.49ml/s with 17.1psi vacuum @16:09:01.25 Cartridge 39 filtering 159/175ml at 0.45ml/s with 28.2psi vacuum @16:09:39.92 Abs.stop @16:09:40.09 Intake.close @16:09:40.21 Exhaust.close @16:09:40.43 Toroid.in :hold @16:09:40.51 Toroid.seek :bypass @16:09:42.17 Toroid.in :default @16:09:42.27 Toroid.seek :clear @16:09:43.91 Sampled 175.0ml @16:09:44.36 GoodNews.email "After filtering 175.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 39" @16:09:45.54 Plunge.seek :reagent @16:09:46.37 Sample loop closed. Ready to Deliver Reagents @16:09:46.39 Archive.in :default @16:09:46.52 Archive.seek :sealed @16:09:49.93 upsync @16:09:50.50 Archive.seek :evac @16:10:04.80 delay 1:00 @16:11:04.85 Archive.seek :clear @16:11:22.59 RNAlater soak for 10:00 @16:21:22.63 Archive.seek :tVent @16:21:25.19 delay 1 second @16:21:26.22 Archive.seek :evac @16:21:41.91 delay 10 seconds @16:21:51.95 Archive.seek :tVent @16:22:07.64 delay 1 second @16:22:08.67 Archive.seek :evac @16:22:24.40 delay 10 seconds @16:22:34.43 Sample preserved and evacuated @16:22:34.59 Selecting Cartridge 37 @16:22:34.89 Carousel.in :default @16:22:35.03 Carousel.home.seek 39 @16:22:35.41 Archive.in :unlocking @16:22:35.56 Archive.seek :clear @16:22:35.69 Air.in :unlocking @16:22:35.84 Air.seek :clear @16:22:40.14 Lysis1.in :unlocking @16:22:40.32 Lysis1.seek :clear @16:22:40.42 Plunge.in :unlocking @16:22:40.54 Plunge.seek :clear @16:22:41.97 Carousel.seek 37 @16:22:42.77 Lysis1.in :fast @16:22:42.85 Lysis1.seek :locked @16:22:45.51 Plunge.in :default @16:22:45.64 Plunge.seek :locked @16:22:45.78 Air.in :fast @16:22:45.87 Air.seek :locked @16:22:46.87 Carousel.reconfigure :locked @16:22:47.06 Powering cartridge bus @16:22:49.72 Gate.setIR 1750,5000,0,6 @16:22:50.38 Gate.setIR 0,5000,0,6 @16:22:50.50 Gate.power :cartridge,:OFF @16:22:51.06 Mounted archive Cartridge @16:22:51.14 GoodNews.email "BACsampler filtering up to 175 ml",Subject: "Sampling archiveHiBiomass_bac cartridge in slot 37" @16:22:51.48 Preparing to filter cartridge 37 @16:22:51.84 Priming sample loop w/150ml, bypass w/50ml @16:22:51.86 SamplePump.setPosition! 0ml @16:22:51.94 Bypass.open @16:22:53.46 Intake.open @16:22:54.25 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:22:56.83 upsync @16:22:56.95 Vacuum tare is 52% of full scale at 24C (drifted 1%) @16:22:57.13 Abs.in :default @16:22:57.22 Abs.hold @16:22:57.50 SamplePump.in :start @16:22:57.60 SamplePump.seek 50ml,3:50 @16:22:58.19 SamplePump.configure :prime @16:23:59.00 Abs.stop @16:23:59.08 SamplePump.setPosition! 0ml @16:23:59.17 Exhaust.open @16:24:00.57 Bypass.close @16:24:02.10 Abs.in :default @16:24:02.19 Abs.hold @16:24:02.53 SamplePump.in :start @16:24:02.63 SamplePump.seek 150ml,10:30 @16:24:03.22 SamplePump.configure :prime @16:27:06.08 Abs.stop @16:27:06.21 Bypass.open @16:27:06.90 Intake.close @16:27:07.75 Toroid.in :hold @16:27:07.82 Toroid.seek :crack @16:27:09.42 Toroid.seek :cartridge @16:27:10.66 Toroid.in :default @16:27:10.77 Toroid.seek :clear @16:27:13.09 Pressurizing cartridge to 26psi above 15.8psia @16:27:13.11 SamplePump.setPosition! 0ml @16:27:13.24 Intake.open @16:27:14.00 Bypass.close @16:27:14.76 Exhaust.close @16:27:15.63 Abs.in :default @16:27:15.75 Abs.hold @16:27:16.07 SamplePump.in :charge @16:27:16.16 SamplePump.seek 2.3ml,42 seconds @16:27:27.81 Abs.stop @16:27:27.90 Intake.close @16:27:28.69 Pressurized cartridge with 0.606ml to 26.2psi @16:27:28.70 Isolated @40.0psia (24.2psi) @16:27:28.76 Verifying cartridge holds at least 0.65..0.85 of 40.0psia @16:27:28.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:27:33.43 Cartridge pressure fell 1% to 39.8psia @16:27:33.55 Sampling 175.0ml @16:27:33.57 SamplePump.setPosition! 0ml @16:27:33.66 Exhaust.open @16:27:34.33 Intake.open @16:27:35.22 Abs.in :default @16:27:35.29 Abs.hold @16:27:35.65 SamplePump.in :start @16:27:35.74 SamplePump.seek 175ml,15:05 @16:27:36.33 SamplePump.configure :sample @16:27:38.78 Cartridge 37 filtering 2/175ml at 0.58ml/s with 16.5psi vacuum 4.2m deep (20.8psia) @16:28:38.84 Cartridge 37 filtering 37/175ml at 0.59ml/s with 21.0psi vacuum @16:29:38.83 Cartridge 37 filtering 71/175ml at 0.56ml/s with 24.8psi vacuum @16:30:38.83 Cartridge 37 filtering 103/175ml at 0.53ml/s with 27.3psi vacuum @16:31:38.83 Cartridge 37 filtering 132/175ml at 0.49ml/s with 22.7psi vacuum 5.8m deep (23.3psia) @16:32:38.83 Cartridge 37 filtering 160/175ml at 0.46ml/s with 28.8psi vacuum @16:33:15.92 Abs.stop @16:33:16.66 Intake.close @16:33:16.74 Exhaust.close @16:33:17.04 Toroid.in :hold @16:33:17.11 Toroid.seek :bypass @16:33:18.80 Toroid.in :default @16:33:18.89 Toroid.seek :clear @16:33:20.54 Sampled 175.0ml @16:33:21.02 GoodNews.email "After filtering 175.0ml",Subject: "Processing archiveHiBiomass_bac cartridge in slot 37" @16:33:21.65 Plunge.seek :reagent @16:33:22.54 Sample loop closed. Ready to Deliver Reagents @16:33:22.60 Archive.in :default @16:33:22.71 Archive.seek :sealed @16:33:26.04 upsync @16:33:26.80 Archive.seek :evac @16:33:41.09 delay 1:00 @16:34:41.13 Archive.seek :clear @16:34:58.89 RNAlater soak for 10:00 @16:44:58.93 Archive.seek :tVent @16:45:01.47 delay 1 second @16:45:02.50 Archive.seek :evac @16:45:18.18 delay 10 seconds @16:45:28.23 Archive.seek :tVent @16:45:43.91 delay 1 second @16:45:44.94 Archive.seek :evac @16:46:00.63 delay 10 seconds @16:46:10.67 Sample preserved and evacuated @16:46:11.00 Carousel.in :default @16:46:11.14 Carousel.home.seek 37 @16:46:11.52 Plunge.seek :locked @16:46:12.44 Archive.in :unlocking @16:46:12.60 Archive.seek :clear @16:46:12.81 Air.in :unlocking @16:46:12.93 Air.seek :clear @16:46:13.07 Lysis1.in :unlocking @16:46:13.20 Lysis1.seek :clear @16:46:17.30 Gate.power :analytic1,:OFF @16:46:17.32 Gate.power :motor,:OFF @16:46:17.36 Gate.power :core,:OFF @16:46:17.40 Gate.power :main,:OFF @16:46:17.43 Safely stopped and ready to power off @16:49:43.74 -> Cmd.stop @16:49:43.80 ready to power off :STOPPED @18:07:46.22UTC20-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @18:07:47.92 >BREAK< ESP3 Gateway v1.43 for core bus @18:07:47.94 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:07:51.44 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @18:07:52.12 Sea pressure (Abs) tare is 6% of full scale @18:08:07.53 Polling Can every 2:00 @18:08:07.58 Waiting for espclients... @18:08:25.04 Session Begun @18:08:36.59 -> ESP.ready! @18:08:36.82 Gate.power :sampler,:OFF,limit to 3500mA @18:08:36.84 Gate.power :skimmer,:OFF,limit to 3500mA @18:08:36.88 Gate.power :analytic1,:ON,limit to 4000mA @18:08:37.60 Plunge.configure :default @18:08:37.77 SamplePump.configure :default @18:08:37.95 Toroid.configure :default @18:08:38.61 Carousel.configure :default @18:08:38.82 Abs.configure :default @18:08:38.98 PhaseSeparator.configure :default @18:08:39.04 SPR Module 1, FW Rev. B, FPGA Rev. C @18:08:39.21 Archive.configure :default @18:08:39.38 Air.configure :default @18:08:39.53 Lysis1.configure :default @18:08:39.67 Lysis2.configure :default @18:08:39.84 PinPort.configure :default @18:08:39.92 All elf controllers running firmware version 4.92 @18:08:40.23 Plunge.in :default @18:08:40.57 Plunge.home.jog 0 @18:08:40.95 PinPort.in :default @18:08:41.02 Lysis1.in :default @18:08:41.05 Archive.in :default @18:08:41.10 Toroid.in :default @18:08:41.15 Lysis2.in :default @18:08:41.19 Air.in :default @18:08:41.23 Carousel.in :default @18:08:41.58 PinPort.home.jog 0 @18:08:41.62 Lysis1.home.jog 0 @18:08:41.67 Archive.home.jog 0 @18:08:41.71 Toroid.home.jog 0 @18:08:41.75 Lysis2.home.jog 0 @18:08:41.79 Carousel.home.jog 0 @18:08:41.86 Air.home.jog 0 @18:08:42.60 Plunge.in :homing @18:08:42.96 Plunge.jog 160 @18:08:43.03 Lysis2.in :homing @18:08:43.08 PinPort.in :homing @18:08:43.13 Toroid.in :homing @18:08:43.16 Lysis1.in :homing @18:08:43.21 Archive.in :homing @18:08:43.44 PinPort.jog -107 @18:08:43.55 Lysis2.jog 1397 @18:08:43.59 Lysis1.jog 1396 @18:08:43.62 Archive.jog 1395 @18:08:43.69 Toroid.jog 627 @18:08:44.05 PinPort.setRawPosition! 0 @18:08:44.09 Plunge.setRawPosition! 50 @18:08:44.18 Air.in :homing @18:08:44.30 PinPort.seek :home @18:08:44.43 Air.jog 1379 @18:08:44.48 Plunge.seek :home @18:08:44.74 PinPort.in :default @18:08:44.87 Plunge.in :default @18:08:44.98 Lysis2.setRawPosition! 50 @18:08:45.07 Lysis2.seek :home @18:08:45.20 Lysis1.setRawPosition! 50 @18:08:45.22 Archive.setRawPosition! 50 @18:08:45.33 Lysis1.seek :home @18:08:45.45 Archive.seek :home @18:08:45.62 Lysis2.in :default @18:08:45.77 Lysis1.in :default @18:08:45.79 Air.setRawPosition! 50 @18:08:45.93 Air.seek :home @18:08:46.06 Toroid.setRawPosition! 0 @18:08:46.10 Archive.in :default @18:08:46.26 Toroid.seek :home @18:08:46.42 Toroid.in :default @18:08:46.44 Air.in :default @18:08:46.85 Toroid.seek :clear @18:08:48.60 Carousel.in :default @18:08:49.27 Carousel.home.seek 8 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 8 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:08:56.62 -> SPR.start @18:08:57.87 SPR.command "read_sequences" @18:08:57.93 SPR.command "stats_on" # @18:08:58.81 SPR.start /var/log/esp/spr24aug2018h.spr.bz2 @18:09:10.41 -> SPR.comand "start 3 0" @18:09:10.46 NoMethodError in bill -- undefined method `comand' for SPR:AnalyticModule::SPR @18:09:27.63 -> SPR.command "start 3 0" @18:09:27.66 SPR.command "start 3 0" @18:24:19.69 -> SPR.command "start 2 14" @18:24:19.72 SPR.command "start 2 14" @18:24:27.07 -> SPR.stop @18:24:33.15 Ended log spr24aug2018h.spr.bz2 @18:24:33.18 SPR.command "stats_off" @18:37:41.40 -> Cmd.stop @18:37:41.53 Intake.close @18:37:41.61 Exhaust.close @18:37:41.70 Bypass.close @18:37:42.40 Carousel.in :default @18:37:42.54 Carousel.home.seek 8 @18:37:42.99 Lysis1.in :fast @18:37:43.06 Lysis1.seek :locked @18:37:45.62 Plunge.seek :locked @18:37:46.99 Lysis1.in :unlocking @18:37:47.14 Lysis1.seek :clear @18:37:48.66 Gate.power :analytic1,:OFF @18:37:48.68 Gate.power :motor,:OFF @18:37:48.74 Gate.power :core,:OFF @18:37:48.77 Gate.power :main,:OFF @18:37:48.81 Safely stopped and ready to power off :STOPPED @18:41:43.09UTC20-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @18:41:46.83 >BREAK< ESP3 Gateway v1.43 for core bus @18:41:46.85 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:41:50.42 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @18:41:51.15 Sea pressure (Abs) tare is 6% of full scale @18:42:06.06 Polling Can every 2:00 @18:42:06.12 Waiting for espclients... @18:42:14.40 Session Begun @18:42:37.42 -> ESP.ready! @18:42:37.49 Gate.power :sampler,:OFF,limit to 3500mA @18:42:37.50 Gate.power :skimmer,:OFF,limit to 3500mA @18:42:37.53 Gate.power :main,:ON,limit to 5000mA @18:42:37.59 Gate.power :analytic1,:ON,limit to 4000mA @18:42:37.76 Powering core bus @18:42:40.01 SPR Module 1, FW Rev. B, FPGA Rev. C @18:42:40.54 Plunge.configure :default @18:42:40.66 SamplePump.configure :default @18:42:40.79 Toroid.configure :default @18:42:40.89 Carousel.configure :default @18:42:41.45 Abs.configure :default @18:42:41.56 PhaseSeparator.configure :default @18:42:41.67 Archive.configure :default @18:42:41.77 Air.configure :default @18:42:41.85 Lysis1.configure :default @18:42:41.92 Lysis2.configure :default @18:42:42.01 PinPort.configure :default @18:42:42.09 All elf controllers running firmware version 4.92 @18:42:42.12 Gate.power :motor,:ON,limit to 5000mA @18:42:42.36 Plunge.home.jog 0 @18:42:43.05 Lysis1.home.jog 0 @18:42:43.19 Lysis2.home.jog 0 @18:42:43.27 Archive.home.jog 0 @18:42:43.30 Toroid.home.jog 0 @18:42:43.34 Carousel.home.jog 0 @18:42:43.38 PinPort.home.jog 0 @18:42:43.42 Air.home.jog 0 @18:42:44.22 Plunge.in :homing @18:42:44.57 Plunge.jog 1608 @18:42:44.65 Lysis2.in :homing @18:42:44.71 Air.in :homing @18:42:44.79 Toroid.in :homing @18:42:44.84 PinPort.in :homing @18:42:44.88 Archive.in :homing @18:42:44.93 Lysis1.in :homing @18:42:45.22 Lysis2.jog 1399 @18:42:45.28 Air.jog 1377 @18:42:45.37 Toroid.jog 626 @18:42:45.44 PinPort.jog -108 @18:42:45.50 Archive.jog 1396 @18:42:45.56 Lysis1.jog 1396 @18:42:45.92 PinPort.setRawPosition! 0 @18:42:46.01 PinPort.seek :home @18:42:46.12 PinPort.in :default @18:42:46.28 Plunge.setRawPosition! 50 @18:42:46.37 Plunge.seek :home @18:42:46.58 Lysis2.setRawPosition! 50 @18:42:46.69 Plunge.in :default @18:42:46.77 Lysis2.seek :home @18:42:46.97 Lysis1.setRawPosition! 50 @18:42:47.02 Air.setRawPosition! 50 @18:42:47.08 Archive.setRawPosition! 50 @18:42:47.20 Lysis1.seek :home @18:42:47.29 Air.seek :home @18:42:47.33 Archive.seek :home @18:42:47.62 Lysis2.in :default @18:42:47.75 Lysis1.in :default @18:42:47.81 Air.in :default @18:42:47.97 Archive.in :default @18:42:48.00 Toroid.setRawPosition! 0 @18:42:48.14 Toroid.seek :home @18:42:48.26 Toroid.in :default @18:42:48.40 Toroid.seek :clear @18:42:50.10 Carousel.in :default @18:42:50.24 Carousel.home.seek 8 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 8 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:43:03.65 -> SC Storage Carousel (SC) at 8 @18:43:08.45 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, [58,56,5] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0510h.spr.bz2"}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 254.6}, [53,52] => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug1423h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug0520h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr24aug0122h.spr.bz2"}, 59 => { Type: :lyseHold_bac, State: :incompatible, filtered: 50, lysed: 825, sensed: :archive, spr: "spr24aug0120h.spr.bz2", was: :processed}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @18:46:03.91 -> slot [59..50] =>[:microSPR_phyto, :standard, {lysed:1100, lysed2:1100}] {59..50 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @18:47:59.77 -> runStandard # @18:48:00.71 Toroid.seek :clear @18:48:00.95 Carousel.in :default @18:48:01.10 Carousel.home.seek 8 @18:48:01.40 Selecting Cartridge 59 @18:48:01.71 Carousel.seek 59 @18:48:12.44 Lysis1.in :fast @18:48:12.53 Lysis1.seek :locked @18:48:15.16 Plunge.seek :locked @18:48:15.35 Air.in :fast @18:48:15.42 Air.seek :locked @18:48:16.43 Carousel.reconfigure :locked @18:48:16.62 Powering cartridge bus @18:48:19.28 Gate.setIR 1750,5000,0,6 @18:48:19.41 Gate.setIR 0,5000,0,6 @18:48:19.50 PuckHeater.configure :default @18:48:19.60 Gate.power :heat,:ON,limit to 5000mA @18:48:20.26 Mounted lysis Cartridge @18:48:20.31 WARNING: Using default puck temperature sensor calibration @18:48:20.33 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:48:20.74 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 59" @18:48:22.39 SPR.command "read_sequences" @18:48:23.20 SPR.command "stats_on" @18:48:23.38 SPR.command "start 0 1" @18:48:24.10 SPR.start /var/log/esp/spr24aug2018h#2.spr.bz2 @18:48:26.06 Waiting for baseline SPR stabilization @18:48:26.07 Handoff.select :waste @18:48:26.19 upsync @18:48:26.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:48:32.92 Syringe tare is 51% of full scale at 22C (drifted 0%) @18:48:32.95 PinPort.in :locking @18:48:33.07 PinPort.seek :locked @18:48:33.63 Archive.in :fast @18:48:33.73 Lysis2.in :fast @18:48:33.80 Lysis1.seek 1100uL @18:48:33.85 Archive.seek :full @18:48:34.11 Lysis2.seek 1100uL @18:48:38.40 delayUntil "18:58:25.60" @18:58:25.61 Lysis2.in :DAmix @18:58:25.82 Lysis1.in :DAmix @18:58:26.47 Lysis2.seek 950uL @18:58:26.53 Lysis1.seek 950uL @18:58:47.44 delay 5 seconds @18:58:52.46 Handoff.select SPR @18:58:52.62 SPR.command "start 2 14" @18:58:54.09 SPR stabilized @18:58:54.28 delay 10 seconds @18:59:04.30 SPR.command "start 2 0" @18:59:07.97 Waiting up to 11:00 for SPR to enter state 1 @19:01:06.06 SPR.command "hold_on" @19:01:07.45 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @19:01:08.10 Lysis2.seek 500uL @19:01:08.16 Lysis1.seek 500uL @19:02:10.45 delay 15 seconds @19:02:25.49 SPR.command "hold_off" @19:02:28.92 Waiting up to 1:00:00 for SPR to enter state 7 @19:18:44.47 SPRsummary:-22.7RIU,25.5RIU,32.8RIU,-24.4RIU after 1922s @19:33:01.53 delay 10 seconds @19:33:11.55 SPR.command "start 2 0" @19:33:14.24 Waiting up to 11:00 for SPR to enter state 1 @19:35:11.92 SPR.command "hold_on" @19:35:13.30 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @19:35:13.93 Lysis2.seek 275uL @19:35:14.04 Lysis1.seek 275uL @19:35:45.20 delay 15 seconds @19:36:00.23 SPR.command "hold_off" @19:36:03.55 Waiting up to 1:00:00 for SPR to enter state 7 @19:46:53.51 Session Begun @19:46:56.21 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 58..50 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}, 59 => { Type: :microSPR_phyto, State: :processing, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @19:47:41.80 -> Cartridge.reserve [58..50] 9 @19:47:46.60 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 58..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 59 => { Type: :microSPR_phyto, State: :processing, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @19:48:12.51 -> Cartridge.release 58 1 @20:06:36.17 delay 10 seconds @20:06:46.19 SPR.command "start 2 0" @20:06:48.88 Waiting up to 11:00 for SPR to enter state 1 @20:08:46.54 SPR.command "hold_on" @20:08:48.45 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @20:08:49.18 Lysis2.seek 50uL @20:08:49.24 Lysis1.seek 50uL @20:09:20.51 delay 15 seconds @20:09:35.53 SPR.command "hold_off" @20:09:38.18 Waiting up to 1:00:00 for SPR to enter state 7 @20:40:09.70 SPR analysis completed @20:40:25.33 Ended log spr24aug2018h#2.spr.bz2 @20:40:25.37 SPR.command "stats_off" @20:50:47.13 -> runStandard # @20:50:47.36 Selecting Cartridge 58 @20:50:47.61 Gate.power :heat,:OFF @20:50:47.64 Gate.power :cartridge,:OFF @20:50:47.79 PinPort.in :unlocking @20:50:47.89 PinPort.seek :clear @20:50:48.41 Carousel.in :default @20:50:48.57 Carousel.home.seek 59 @20:50:48.95 Archive.in :unlocking @20:50:49.09 Archive.seek :clear @20:50:49.29 Air.in :unlocking @20:50:49.46 Air.seek :clear @20:50:49.61 Lysis2.in :unlocking @20:50:49.71 Lysis2.seek :clear @20:50:54.18 Lysis1.in :unlocking @20:50:54.34 Lysis1.seek :clear @20:50:54.48 Plunge.in :unlocking @20:50:54.57 Plunge.seek :clear @20:50:58.78 Carousel.seek 58 @20:50:59.37 Lysis1.in :fast @20:50:59.47 Lysis1.seek :locked @20:51:02.13 Plunge.in :default @20:51:02.23 Plunge.seek :locked @20:51:02.45 Air.in :fast @20:51:02.54 Air.seek :locked @20:51:03.55 Carousel.reconfigure :locked @20:51:03.75 Powering cartridge bus @20:51:06.41 Gate.setIR 1750,5000,0,6 @20:51:06.54 Gate.setIR 0,5000,0,6 @20:51:06.64 PuckHeater.configure :default @20:51:06.79 Gate.power :heat,:ON,limit to 5000mA @20:51:07.41 Mounted lysis Cartridge @20:51:07.45 WARNING: Using default puck temperature sensor calibration @20:51:07.50 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:51:08.35 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 58" @20:51:08.94 SPR.command "stats_on" @20:51:09.51 SPR.command "start 0 1" @20:51:10.18 SPR.start /var/log/esp/spr24aug2020h.spr.bz2 @20:51:12.24 Waiting for baseline SPR stabilization @20:51:12.32 Handoff.select :waste @20:51:12.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:51:14.21 upsync @20:51:19.44 Syringe tare is 51% of full scale at 24C (drifted 0%) @20:51:19.48 PinPort.in :locking @20:51:19.63 PinPort.seek :locked @20:51:20.19 Archive.in :fast @20:51:20.31 Lysis2.in :fast @20:51:20.45 Lysis1.seek 1100uL @20:51:20.56 Archive.seek :full @20:51:20.64 Lysis2.seek 1100uL @20:51:25.06 delayUntil "21:01:12.09" @21:01:12.10 Lysis2.in :DAmix @21:01:12.35 Lysis1.in :DAmix @21:01:13.11 Lysis2.seek 950uL @21:01:13.17 Lysis1.seek 950uL @21:01:34.08 delay 5 seconds @21:01:39.13 Handoff.select SPR @21:01:39.31 SPR.command "start 2 14" @21:01:40.81 SPR stabilized @21:01:41.02 delay 10 seconds @21:01:51.04 SPR.command "start 2 0" @21:01:53.63 Waiting up to 11:00 for SPR to enter state 1 @21:03:51.29 SPR.command "hold_on" @21:03:52.67 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @21:03:53.40 Lysis2.seek 500uL @21:03:53.53 Lysis1.seek 500uL @21:04:55.69 delay 15 seconds @21:05:10.73 SPR.command "hold_off" @21:05:14.14 Waiting up to 1:00:00 for SPR to enter state 7 @21:21:29.70 SPRsummary:-1.4RIU,254RIU,311RIU,-1.88RIU after 9011s @21:35:47.25 delay 10 seconds @21:35:57.27 SPR.command "start 2 0" @21:36:00.41 Waiting up to 11:00 for SPR to enter state 1 @21:37:58.08 SPR.command "hold_on" @21:37:59.46 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @21:38:00.11 Lysis2.seek 275uL @21:38:00.22 Lysis1.seek 275uL @21:38:31.39 delay 15 seconds @21:38:46.41 SPR.command "hold_off" @21:38:49.71 Waiting up to 1:00:00 for SPR to enter state 7 @21:45:51.26 Session Begun @21:45:53.59 -> showlog @22:09:22.35 delay 10 seconds @22:09:32.37 SPR.command "start 2 0" @22:09:35.04 Waiting up to 11:00 for SPR to enter state 1 @22:11:32.70 SPR.command "hold_on" @22:11:34.09 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @22:11:34.86 Lysis2.seek 50uL @22:11:34.93 Lysis1.seek 50uL @22:12:06.18 delay 15 seconds @22:12:21.20 SPR.command "hold_off" @22:12:24.33 Waiting up to 1:00:00 for SPR to enter state 7 @22:32:42.89 Session Ended @22:42:56.81 SPR analysis completed @22:43:12.40 Ended log spr24aug2020h.spr.bz2 @22:43:12.42 SPR.command "stats_off" @23:34:03.27 Session Begun @23:34:07.36 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 57..50 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @23:49:17.31 -> Cartridge.release 50 1 @23:49:26.26 -> runStandard @23:49:26.49 Selecting Cartridge 50 # @23:49:26.83 Gate.power :heat,:OFF @23:49:26.85 Gate.power :cartridge,:OFF @23:49:27.05 PinPort.in :unlocking @23:49:27.18 PinPort.seek :clear @23:49:27.70 Carousel.in :default @23:49:27.89 Carousel.home.seek 58 @23:49:28.32 Archive.in :unlocking @23:49:28.49 Archive.seek :clear @23:49:28.66 Air.in :unlocking @23:49:28.89 Air.seek :clear @23:49:29.07 Lysis2.in :unlocking @23:49:29.20 Lysis2.seek :clear @23:49:33.70 Lysis1.in :unlocking @23:49:33.85 Lysis1.seek :clear @23:49:34.05 Plunge.in :unlocking @23:49:34.17 Plunge.seek :clear @23:49:38.28 Carousel.seek 50 @23:49:40.29 Lysis1.in :fast @23:49:40.37 Lysis1.seek :locked @23:49:43.07 Plunge.in :default @23:49:43.23 Plunge.seek :locked @23:49:43.89 Air.in :fast @23:49:44.00 Air.seek :locked @23:49:45.02 Carousel.reconfigure :locked @23:49:45.26 Powering cartridge bus @23:49:47.93 Gate.setIR 1750,5000,0,6 @23:49:48.06 Gate.setIR 0,5000,0,6 @23:49:48.18 PuckHeater.configure :default @23:49:48.32 Gate.power :heat,:ON,limit to 5000mA @23:49:48.94 Mounted lysis Cartridge @23:49:49.01 WARNING: Using default puck temperature sensor calibration @23:49:49.07 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:49:49.48 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 50" @23:49:50.04 SPR.command "stats_on" @23:49:50.51 SPR.command "start 0 1" @23:49:51.21 SPR.start /var/log/esp/spr24aug2023h.spr.bz2 @23:49:53.28 Waiting for baseline SPR stabilization @23:49:53.33 Handoff.select :waste @23:49:53.80 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:49:55.35 upsync @23:50:00.45 Syringe tare is 51% of full scale at 25C (drifted 0%) @23:50:00.54 PinPort.in :locking @23:50:00.67 PinPort.seek :locked @23:50:01.32 Archive.in :fast @23:50:01.39 Lysis2.in :fast @23:50:01.57 Lysis1.seek 1100uL @23:50:01.66 Lysis2.seek 1100uL @23:50:01.74 Archive.seek :full @23:50:06.15 delayUntil "23:59:53.13" @23:59:53.14 Lysis2.in :DAmix @23:59:53.33 Lysis1.in :DAmix @23:59:53.92 Lysis2.seek 950uL @23:59:53.98 Lysis1.seek 950uL @00:00:14.89UTC21-Aug-24 delay 5 seconds @00:00:19.91 Handoff.select SPR @00:00:20.11 SPR.command "start 2 14" @00:00:21.28 SPR stabilized @00:00:21.50 delay 10 seconds @00:00:31.53 SPR.command "start 2 0" @00:00:34.64 Waiting up to 11:00 for SPR to enter state 1 @00:02:32.31 SPR.command "hold_on" @00:02:34.22 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @00:02:34.95 Lysis2.seek 500uL @00:02:35.01 Lysis1.seek 500uL @00:03:37.29 delay 15 seconds @00:03:52.31 SPR.command "hold_off" @00:03:55.68 Waiting up to 1:00:00 for SPR to enter state 7 @00:20:10.74 SPRsummary:12.1RIU,220RIU,257RIU,13.6RIU after 16102s @00:34:27.76 delay 10 seconds @00:34:37.79 SPR.command "start 2 0" @00:34:41.00 Waiting up to 11:00 for SPR to enter state 1 @00:36:39.09 SPR.command "hold_on" @00:36:40.48 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @00:36:41.27 Lysis2.seek 275uL @00:36:41.40 Lysis1.seek 275uL @00:37:12.58 delay 15 seconds @00:37:27.61 SPR.command "hold_off" @00:37:31.26 Waiting up to 1:00:00 for SPR to enter state 7 @01:08:04.29 delay 10 seconds @01:08:14.31 SPR.command "start 2 0" @01:08:17.00 Waiting up to 11:00 for SPR to enter state 1 @01:10:14.66 SPR.command "hold_on" @01:10:16.04 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @01:10:16.79 Lysis2.seek 50uL @01:10:16.86 Lysis1.seek 50uL @01:10:48.11 delay 15 seconds @01:11:03.13 SPR.command "hold_off" @01:11:06.29 Waiting up to 1:00:00 for SPR to enter state 7 @01:12:26.89 Session Ended @01:41:38.79 SPR analysis completed @01:41:54.40 Ended log spr24aug2023h.spr.bz2 @01:41:54.42 SPR.command "stats_off" @01:54:41.32 Session Begun @01:54:45.95 -> showlog -50 @01:54:54.75 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 57..51 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @02:19:23.93 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 57..51 => { Type: :microSPR_phyto, State: :reserved, lysed: 1100, lysed2: 1100, was: :standard}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @02:20:20.07 -> Cartridge.release [57..51] 7 @02:20:39.45 -> Cmd.stop @02:20:39.64 Handoff.select :close @02:20:39.86 Gate.power :heat,:OFF @02:20:39.89 Gate.power :cartridge,:OFF @02:20:40.07 PinPort.in :unlocking @02:20:40.20 PinPort.seek :clear @02:20:40.77 Carousel.in :default @02:20:40.96 Carousel.home.seek 50 @02:20:41.36 Plunge.seek :locked @02:20:41.61 Archive.in :unlocking @02:20:41.80 Archive.seek :clear @02:20:41.97 Air.in :unlocking @02:20:42.19 Air.seek :clear @02:20:42.33 Lysis1.in :unlocking @02:20:43.11 Lysis1.seek :clear @02:20:43.31 Lysis2.in :unlocking @02:20:43.44 Lysis2.seek :clear @02:20:48.03 Gate.power :analytic1,:OFF @02:20:48.05 Gate.power :motor,:OFF @02:20:48.13 Gate.power :core,:OFF @02:20:48.18 Gate.power :main,:OFF @02:20:48.25 Safely stopped and ready to power off :STOPPED @02:21:02.78 -> exit @02:21:02.82 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @02:23:38.03 Session Begun @02:24:08.45 -> showlog @02:24:51.04 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 57..51 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @02:25:04.68 -> showlog -50 @02:25:30.32 -> Cmd.stop @02:25:30.41 ready to power off :STOPPED Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @02:26:02.31 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 57..51 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @02:26:21.64 -> runStandard # @02:26:21.94 Gate.power :sampler,:OFF,limit to 3500mA @02:26:21.98 Gate.power :skimmer,:OFF,limit to 3500mA @02:26:22.04 Gate.power :main,:ON,limit to 5000mA @02:26:22.09 Gate.power :analytic1,:ON,limit to 4000mA @02:26:22.24 Powering core bus @02:26:24.56 SPR Module 1, FW Rev. B, FPGA Rev. C @02:26:25.02 Plunge.configure :default @02:26:25.18 SamplePump.configure :default @02:26:25.33 Toroid.configure :default @02:26:25.48 Carousel.configure :default @02:26:25.61 Abs.configure :default @02:26:25.76 PhaseSeparator.configure :default @02:26:25.91 Archive.configure :default @02:26:26.05 Air.configure :default @02:26:26.15 Lysis1.configure :default @02:26:26.27 Lysis2.configure :default @02:26:26.40 PinPort.configure :default @02:26:26.52 All elf controllers running firmware version 4.92 @02:26:26.56 Gate.power :motor,:ON,limit to 5000mA @02:26:27.08 Plunge.home.jog 0 @02:26:27.67 Lysis1.home.jog 0 @02:26:27.85 Lysis2.home.jog 0 @02:26:27.92 Air.home.jog 0 @02:26:27.99 Carousel.home.jog 0 @02:26:28.04 Toroid.home.jog 0 @02:26:28.17 PinPort.home.jog 0 @02:26:28.29 Archive.home.jog 0 @02:26:29.24 Plunge.in :homing @02:26:29.69 Plunge.jog 1603 @02:26:29.80 Lysis2.in :homing @02:26:29.87 Air.in :homing @02:26:29.94 Toroid.in :homing @02:26:30.00 PinPort.in :homing @02:26:30.06 Archive.in :homing @02:26:30.19 Lysis1.in :homing @02:26:30.58 Air.jog 1375 @02:26:30.63 Lysis2.jog 1403 @02:26:30.72 PinPort.jog -110 @02:26:30.83 Archive.jog 1401 @02:26:30.93 Toroid.jog 627 @02:26:31.00 Lysis1.jog 1397 @02:26:31.57 PinPort.setRawPosition! 0 @02:26:31.67 Plunge.setRawPosition! 50 @02:26:31.75 PinPort.seek :home @02:26:31.98 Plunge.seek :home @02:26:32.85 Lysis2.setRawPosition! 50 @02:26:32.92 Lysis1.setRawPosition! 50 @02:26:32.98 PinPort.in :default @02:26:33.12 Air.setRawPosition! 50 @02:26:33.18 Archive.setRawPosition! 50 @02:26:33.39 Lysis2.seek :home @02:26:33.52 Lysis1.seek :home @02:26:33.65 Air.seek :home @02:26:33.88 Archive.seek :home @02:26:34.26 Lysis2.in :default @02:26:34.31 Lysis1.in :default @02:26:34.36 Toroid.setRawPosition! 0 @02:26:34.42 Plunge.in :default @02:26:34.78 Toroid.seek :home @02:26:35.00 Archive.in :default @02:26:35.04 Toroid.in :default @02:26:35.12 Air.in :default @02:26:35.63 Toroid.seek :clear @02:26:37.42 Carousel.in :default @02:26:37.60 Carousel.home.seek 50 @02:26:37.93 Selecting Cartridge 57 @02:26:38.30 Carousel.seek 57 @02:26:40.13 Lysis1.in :fast @02:26:40.25 Lysis1.seek :locked @02:26:42.93 Plunge.seek :locked @02:26:43.15 Air.in :fast @02:26:43.26 Air.seek :locked @02:26:44.29 Carousel.reconfigure :locked @02:26:44.52 Powering cartridge bus @02:26:47.18 Gate.setIR 1750,5000,0,6 @02:26:47.31 Gate.setIR 0,5000,0,6 @02:26:47.43 PuckHeater.configure :default @02:26:47.57 Gate.power :heat,:ON,limit to 5000mA @02:26:48.20 Mounted lysis Cartridge @02:26:48.28 WARNING: Using default puck temperature sensor calibration @02:26:48.36 Using default cartridge heat loss curve for HeatingAccelInCarousel @02:26:48.77 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 57" @02:26:50.42 SPR.command "read_sequences" @02:26:50.94 SPR.command "stats_on" @02:26:51.21 SPR.command "start 0 1" @02:26:51.87 SPR.start /var/log/esp/spr24aug2102h.spr.bz2 @02:26:54.93 upsync @02:26:54.97 Waiting for baseline SPR stabilization @02:26:55.06 Handoff.select :waste @02:26:55.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:27:01.96 Syringe tare is 52% of full scale at 25C (drifted 0%) @02:27:02.03 PinPort.in :locking @02:27:02.18 PinPort.seek :locked @02:27:02.78 Archive.in :fast @02:27:02.91 Lysis2.in :fast @02:27:03.02 Lysis1.seek 1100uL @02:27:03.09 Archive.seek :full @02:27:03.38 Lysis2.seek 1100uL @02:27:07.69 delayUntil "02:36:54.27" @02:36:54.29 Lysis2.in :DAmix @02:36:54.47 Lysis1.in :DAmix @02:36:55.15 Lysis2.seek 950uL @02:36:55.30 Lysis1.seek 950uL @02:37:16.15 delay 5 seconds @02:37:21.17 Handoff.select SPR @02:37:21.39 SPR.command "start 2 14" @02:37:22.79 SPR stabilized @02:37:23.04 delay 10 seconds @02:37:33.07 SPR.command "start 2 0" @02:37:36.14 Waiting up to 11:00 for SPR to enter state 1 @02:39:33.81 SPR.command "hold_on" @02:39:35.19 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @02:39:35.90 Lysis2.seek 500uL @02:39:35.97 Lysis1.seek 500uL @02:40:38.27 delay 15 seconds @02:40:53.29 SPR.command "hold_off" @02:40:56.65 Waiting up to 1:00:00 for SPR to enter state 7 @02:57:12.22 SPRsummary:4.81RIU,220RIU,260RIU,7.11RIU after 1922s @03:11:29.25 delay 10 seconds @03:11:39.28 SPR.command "start 2 0" @03:11:42.49 Waiting up to 11:00 for SPR to enter state 1 @03:13:40.59 SPR.command "hold_on" @03:13:42.49 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @03:13:43.26 Lysis2.seek 275uL @03:13:43.32 Lysis1.seek 275uL @03:14:14.66 delay 15 seconds @03:14:29.68 SPR.command "hold_off" @03:14:32.22 Waiting up to 1:00:00 for SPR to enter state 7 @03:36:51.52 Session Ended @03:45:03.88 delay 10 seconds @03:45:13.90 SPR.command "start 2 0" @03:45:16.60 Waiting up to 11:00 for SPR to enter state 1 @03:47:04.14 Session Begun @03:47:07.24 -> showlog @03:47:14.25 SPR.command "hold_on" @03:47:15.12 -> sholog -60 @03:47:15.16 NoMethodError in bill#3 -- undefined method `sholog' for CmdServer::Client:Class @03:47:16.17 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @03:47:16.91 Lysis2.seek 50uL @03:47:16.98 Lysis1.seek 50uL @03:47:48.24 delay 15 seconds @03:48:03.29 SPR.command "hold_off" @03:48:05.89 Waiting up to 1:00:00 for SPR to enter state 7 @04:18:37.42 SPR analysis completed @04:18:53.89 Ended log spr24aug2102h.spr.bz2 @04:18:53.91 SPR.command "stats_off" @04:21:04.46 -> runStandard, runStandard @04:21:04.49 SyntaxError in bill#3 -- compile error bill#3:3: syntax error, unexpected $end, expecting '=' runStandard, runStandard ^ @04:21:34.86 -> runStandard # @04:21:35.11 Selecting Cartridge 56 @04:21:35.37 Gate.power :heat,:OFF @04:21:35.39 Gate.power :cartridge,:OFF @04:21:35.57 PinPort.in :unlocking @04:21:35.69 PinPort.seek :clear @04:21:36.22 Carousel.in :default @04:21:36.40 Carousel.home.seek 57 @04:21:36.84 Archive.in :unlocking @04:21:37.04 Archive.seek :clear @04:21:37.22 Air.in :unlocking @04:21:37.43 Air.seek :clear @04:21:37.60 Lysis2.in :unlocking @04:21:37.73 Lysis2.seek :clear @04:21:42.24 Lysis1.in :unlocking @04:21:42.42 Lysis1.seek :clear @04:21:42.58 Plunge.in :unlocking @04:21:42.69 Plunge.seek :clear @04:21:46.84 Carousel.seek 56 @04:21:47.46 Lysis1.in :fast @04:21:47.59 Lysis1.seek :locked @04:21:50.26 Plunge.in :default @04:21:50.41 Plunge.seek :locked @04:21:50.59 Air.in :fast @04:21:50.71 Air.seek :locked @04:21:51.79 Carousel.reconfigure :locked @04:21:52.05 Powering cartridge bus @04:21:54.75 Gate.setIR 1750,5000,0,6 @04:21:54.89 Gate.setIR 0,5000,0,6 @04:21:55.01 PuckHeater.configure :default @04:21:55.15 Gate.power :heat,:ON,limit to 5000mA @04:21:55.77 Mounted lysis Cartridge @04:21:55.85 WARNING: Using default puck temperature sensor calibration @04:21:55.89 Using default cartridge heat loss curve for HeatingAccelInCarousel @04:21:56.29 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 56" @04:21:56.93 SPR.command "stats_on" @04:21:57.56 SPR.command "start 0 1" @04:21:58.24 SPR.start /var/log/esp/spr24aug2104h.spr.bz2 @04:22:00.26 Waiting for baseline SPR stabilization @04:22:00.29 Handoff.select :waste @04:22:00.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:22:06.94 upsync @04:22:07.18 Syringe tare is 52% of full scale at 25C (drifted 0%) @04:22:07.27 PinPort.in :locking @04:22:07.41 PinPort.seek :locked @04:22:07.98 Archive.in :fast @04:22:08.13 Lysis2.in :fast @04:22:08.19 Archive.seek :full @04:22:08.27 Lysis1.seek 1100uL @04:22:08.65 Lysis2.seek 1100uL @04:22:12.97 delayUntil "04:31:59.91" @04:31:34.79 Session Ended @04:31:59.93 Lysis2.in :DAmix @04:32:00.10 Lysis1.in :DAmix @04:32:00.67 Lysis2.seek 950uL @04:32:00.72 Lysis1.seek 950uL @04:32:21.69 delay 5 seconds @04:32:26.74 Handoff.select SPR @04:32:26.97 SPR.command "start 2 14" @04:32:28.29 SPR stabilized @04:32:28.50 delay 10 seconds @04:32:38.52 SPR.command "start 2 0" @04:32:41.66 Waiting up to 11:00 for SPR to enter state 1 @04:34:39.31 SPR.command "hold_on" @04:34:40.71 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @04:34:41.42 Lysis2.seek 500uL @04:34:41.49 Lysis1.seek 500uL @04:35:43.72 delay 15 seconds @04:35:58.76 SPR.command "hold_off" @04:36:02.17 Waiting up to 1:00:00 for SPR to enter state 7 @04:52:17.73 SPRsummary:-2.09RIU,205RIU,242RIU,-0.149RIU after 9011s @05:06:34.82 delay 10 seconds @05:06:44.84 SPR.command "start 2 0" @05:06:47.48 Waiting up to 11:00 for SPR to enter state 1 @05:08:45.15 SPR.command "hold_on" @05:08:46.54 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @05:08:47.30 Lysis2.seek 275uL @05:08:47.34 Lysis1.seek 275uL @05:09:18.59 delay 15 seconds @05:09:33.61 SPR.command "hold_off" @05:09:36.80 Waiting up to 1:00:00 for SPR to enter state 7 @05:40:09.40 delay 10 seconds @05:40:19.42 SPR.command "start 2 0" @05:40:22.63 Waiting up to 11:00 for SPR to enter state 1 @05:42:20.74 SPR.command "hold_on" @05:42:22.11 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @05:42:22.83 Lysis2.seek 50uL @05:42:22.90 Lysis1.seek 50uL @05:42:54.11 delay 15 seconds @05:43:09.13 SPR.command "hold_off" @05:43:12.36 Waiting up to 1:00:00 for SPR to enter state 7 @06:13:44.82 SPR analysis completed @06:14:00.87 Ended log spr24aug2104h.spr.bz2 @06:14:00.89 SPR.command "stats_off" @07:50:54.24 Session Begun @07:51:03.89 -> sowlog -80 @07:51:03.97 NoMethodError in bill#3 -- undefined method `sowlog' for CmdServer::Client:Class @07:51:11.42 -> showlog -80 @07:51:42.13 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 55..51 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}, 56 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2104h.spr.bz2}, 57 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2102h.spr.bz2}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @07:52:34.34 -> runStandard # @07:52:34.58 Selecting Cartridge 55 @07:52:34.87 Gate.power :heat,:OFF @07:52:34.91 Gate.power :cartridge,:OFF @07:52:35.09 PinPort.in :unlocking @07:52:35.21 PinPort.seek :clear @07:52:35.73 Carousel.in :default @07:52:35.91 Carousel.home.seek 56 @07:52:36.32 Archive.in :unlocking @07:52:36.51 Archive.seek :clear @07:52:36.69 Air.in :unlocking @07:52:36.92 Air.seek :clear @07:52:37.00 Lysis2.in :unlocking @07:52:37.20 Lysis2.seek :clear @07:52:41.72 Lysis1.in :unlocking @07:52:41.91 Lysis1.seek :clear @07:52:42.05 Plunge.in :unlocking @07:52:42.19 Plunge.seek :clear @07:52:46.39 Carousel.seek 55 @07:52:47.25 Lysis1.in :fast @07:52:47.37 Lysis1.seek :locked @07:52:50.04 Plunge.in :default @07:52:50.20 Plunge.seek :locked @07:52:50.40 Air.in :fast @07:52:50.51 Air.seek :locked @07:52:51.55 Carousel.reconfigure :locked @07:52:51.77 Powering cartridge bus @07:52:54.44 Gate.setIR 1750,5000,0,6 @07:52:54.58 Gate.setIR 0,5000,0,6 @07:52:54.70 PuckHeater.configure :default @07:52:54.88 Gate.power :heat,:ON,limit to 5000mA @07:52:55.50 Mounted lysis Cartridge @07:52:55.59 WARNING: Using default puck temperature sensor calibration @07:52:55.63 Using default cartridge heat loss curve for HeatingAccelInCarousel @07:52:56.05 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 55" @07:52:56.62 SPR.command "stats_on" @07:52:57.50 SPR.command "start 0 1" @07:52:58.16 SPR.start /var/log/esp/spr24aug2107h.spr.bz2 @07:53:00.80 Waiting for baseline SPR stabilization @07:53:00.87 Handoff.select :waste @07:53:01.39 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @07:53:02.64 upsync @07:53:08.08 Syringe tare is 52% of full scale at 26C (drifted 0%) @07:53:08.15 PinPort.in :locking @07:53:08.32 PinPort.seek :locked @07:53:08.89 Archive.in :fast @07:53:09.06 Lysis2.in :fast @07:53:09.13 Lysis1.seek 1100uL @07:53:09.69 Archive.seek :full @07:53:10.07 Lysis2.seek 1100uL @07:53:14.41 delayUntil "08:03:00.68" @08:03:00.70 Lysis2.in :DAmix @08:03:00.92 Lysis1.in :DAmix @08:03:01.55 Lysis2.seek 950uL @08:03:01.68 Lysis1.seek 950uL @08:03:22.56 delay 5 seconds @08:03:27.58 Handoff.select SPR @08:03:27.79 SPR.command "start 2 14" @08:03:29.19 SPR stabilized @08:03:29.42 delay 10 seconds @08:03:39.45 SPR.command "start 2 0" @08:03:42.55 Waiting up to 11:00 for SPR to enter state 1 @08:05:40.21 SPR.command "hold_on" @08:05:41.60 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @08:05:42.29 Lysis2.seek 500uL @08:05:42.35 Lysis1.seek 500uL @08:06:44.63 delay 15 seconds @08:06:59.66 SPR.command "hold_off" @08:07:03.07 Waiting up to 1:00:00 for SPR to enter state 7 @08:15:57.88 Session Ended @08:23:18.62 SPRsummary:8.48RIU,212RIU,238RIU,16.1RIU after 16103s @08:37:35.67 delay 10 seconds @08:37:45.69 SPR.command "start 2 0" @08:37:48.38 Waiting up to 11:00 for SPR to enter state 1 @08:39:46.05 SPR.command "hold_on" @08:39:47.44 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @08:39:48.18 Lysis2.seek 275uL @08:39:48.24 Lysis1.seek 275uL @08:40:19.48 delay 15 seconds @08:40:34.50 SPR.command "hold_off" @08:40:37.69 Waiting up to 1:00:00 for SPR to enter state 7 @09:11:10.29 delay 10 seconds @09:11:20.31 SPR.command "start 2 0" @09:11:23.01 Waiting up to 11:00 for SPR to enter state 1 @09:13:20.67 SPR.command "hold_on" @09:13:22.06 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @09:13:22.79 Lysis2.seek 50uL @09:13:22.85 Lysis1.seek 50uL @09:13:54.10 delay 15 seconds @09:14:09.12 SPR.command "hold_off" @09:14:12.83 Waiting up to 1:00:00 for SPR to enter state 7 @09:44:45.72 SPR analysis completed @09:45:01.21 Ended log spr24aug2107h.spr.bz2 @09:45:01.23 SPR.command "stats_off" @10:01:31.96 Session Begun @10:01:38.73 -> showlog -80 @10:02:20.60 -> runStandard # @10:02:20.89 Selecting Cartridge 54 @10:02:21.16 Gate.power :heat,:OFF @10:02:21.20 Gate.power :cartridge,:OFF @10:02:21.37 PinPort.in :unlocking @10:02:21.51 PinPort.seek :clear @10:02:22.06 Carousel.in :default @10:02:22.24 Carousel.home.seek 55 @10:02:22.65 Archive.in :unlocking @10:02:22.86 Archive.seek :clear @10:02:23.06 Air.in :unlocking @10:02:23.23 Air.seek :clear @10:02:23.41 Lysis2.in :unlocking @10:02:23.53 Lysis2.seek :clear @10:02:28.02 Lysis1.in :unlocking @10:02:28.24 Lysis1.seek :clear @10:02:28.37 Plunge.in :unlocking @10:02:28.49 Plunge.seek :clear @10:02:32.71 Carousel.seek 54 @10:02:33.35 Lysis1.in :fast @10:02:33.48 Lysis1.seek :locked @10:02:36.16 Plunge.in :default @10:02:36.35 Plunge.seek :locked @10:02:36.49 Air.in :fast @10:02:36.61 Air.seek :locked @10:02:37.64 Carousel.reconfigure :locked @10:02:37.87 Powering cartridge bus @10:02:40.53 Gate.setIR 1750,5000,0,6 @10:02:40.67 Gate.setIR 0,5000,0,6 @10:02:40.83 PuckHeater.configure :default @10:02:40.96 Gate.power :heat,:ON,limit to 5000mA @10:02:41.65 Mounted lysis Cartridge board#Q12558 @10:02:41.70 WARNING: Using default puck temperature sensor calibration @10:02:41.77 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:02:42.18 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 54" @10:02:42.79 SPR.command "stats_on" @10:02:43.55 SPR.command "start 0 1" @10:02:44.25 SPR.start /var/log/esp/spr24aug2110h.spr.bz2 @10:02:46.83 Waiting for baseline SPR stabilization @10:02:46.85 Handoff.select :waste @10:02:47.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:02:53.79 Syringe tare is 52% of full scale at 26C (drifted 1%) @10:02:53.84 PinPort.in :locking @10:02:54.04 PinPort.seek :locked @10:02:54.62 Archive.in :fast @10:02:54.84 Lysis1.seek 1100uL @10:02:54.91 Lysis2.in :fast @10:02:54.99 Archive.seek :full @10:02:55.36 Lysis2.seek 1100uL @10:02:55.61 upsync @10:02:59.67 delayUntil "10:12:46.78" @10:12:46.80 Lysis2.in :DAmix @10:12:46.98 Lysis1.in :DAmix @10:12:47.55 Lysis2.seek 950uL @10:12:47.64 Lysis1.seek 950uL @10:13:08.57 delay 5 seconds @10:13:13.59 Handoff.select SPR @10:13:13.80 SPR.command "start 2 14" @10:13:15.26 SPR stabilized @10:13:15.50 delay 10 seconds @10:13:25.52 SPR.command "start 2 0" @10:13:28.62 Waiting up to 11:00 for SPR to enter state 1 @10:15:26.29 SPR.command "hold_on" @10:15:27.69 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @10:15:28.36 Lysis2.seek 500uL @10:15:28.43 Lysis1.seek 500uL @10:16:30.67 delay 15 seconds @10:16:45.71 SPR.command "hold_off" @10:16:49.67 Waiting up to 1:00:00 for SPR to enter state 7 @10:33:05.66 SPRsummary:-3.64RIU,129RIU,167RIU,1.69RIU after 23196s @10:47:22.70 delay 10 seconds @10:47:32.75 SPR.command "start 2 0" @10:47:35.42 Waiting up to 11:00 for SPR to enter state 1 @10:49:33.08 SPR.command "hold_on" @10:49:34.47 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @10:49:35.25 Lysis2.seek 275uL @10:49:35.40 Lysis1.seek 275uL @10:50:06.60 delay 15 seconds @10:50:21.62 SPR.command "hold_off" @10:50:24.72 Waiting up to 1:00:00 for SPR to enter state 7 @11:20:57.34 delay 10 seconds @11:21:07.36 SPR.command "start 2 0" @11:21:10.04 Waiting up to 11:00 for SPR to enter state 1 @11:23:07.69 SPR.command "hold_on" @11:23:09.09 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @11:23:09.82 Lysis2.seek 50uL @11:23:09.91 Lysis1.seek 50uL @11:23:41.17 delay 15 seconds @11:23:56.19 SPR.command "hold_off" @11:23:59.34 Waiting up to 1:00:00 for SPR to enter state 7 @11:50:58.49 Session Ended @11:54:31.81 SPR analysis completed @11:54:47.00 Ended log spr24aug2110h.spr.bz2 @11:54:47.02 SPR.command "stats_off" @11:56:50.52 Session Begun @11:56:56.30 -> showlog -100 @11:57:06.76 -> runStandard # @11:57:07.06 Selecting Cartridge 53 @11:57:07.32 Gate.power :heat,:OFF @11:57:07.35 Gate.power :cartridge,:OFF @11:57:07.54 PinPort.in :unlocking @11:57:07.66 PinPort.seek :clear @11:57:08.20 Carousel.in :default @11:57:08.39 Carousel.home.seek 54 @11:57:08.84 Archive.in :unlocking @11:57:09.05 Archive.seek :clear @11:57:09.17 Air.in :unlocking @11:57:09.40 Air.seek :clear @11:57:09.48 Lysis2.in :unlocking @11:57:09.70 Lysis2.seek :clear @11:57:14.24 Lysis1.in :unlocking @11:57:14.44 Lysis1.seek :clear @11:57:14.58 Plunge.in :unlocking @11:57:14.77 Plunge.seek :clear @11:57:18.93 Carousel.seek 53 @11:57:19.54 Lysis1.in :fast @11:57:19.65 Lysis1.seek :locked @11:57:22.83 Plunge.in :default @11:57:23.01 Plunge.seek :locked @11:57:23.20 Air.in :fast @11:57:23.31 Air.seek :locked @11:57:24.32 Carousel.reconfigure :locked @11:57:24.56 Powering cartridge bus @11:57:27.22 Gate.setIR 1750,5000,0,6 @11:57:27.39 Gate.setIR 0,5000,0,6 @11:57:27.51 PuckHeater.configure :default @11:57:27.65 Gate.power :heat,:ON,limit to 5000mA @11:57:28.28 Mounted lysis Cartridge @11:57:28.38 WARNING: Using default puck temperature sensor calibration @11:57:28.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:57:28.89 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 53" @11:57:29.42 SPR.command "stats_on" @11:57:30.15 SPR.command "start 0 1" @11:57:30.85 SPR.start /var/log/esp/spr24aug2111h.spr.bz2 @11:57:32.90 Waiting for baseline SPR stabilization @11:57:32.93 Handoff.select :waste @11:57:33.21 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:57:34.35 upsync @11:57:39.84 Syringe tare is 52% of full scale at 26C (drifted 0%) @11:57:39.91 PinPort.in :locking @11:57:40.06 PinPort.seek :locked @11:57:40.64 Archive.in :fast @11:57:40.83 Lysis2.in :fast @11:57:40.91 Lysis1.seek 1100uL @11:57:41.12 Archive.seek :full @11:57:41.26 Lysis2.seek 1100uL @11:57:45.67 delayUntil "12:07:32.52" @12:07:07.46 Session Ended @12:07:32.53 Lysis2.in :DAmix @12:07:32.71 Lysis1.in :DAmix @12:07:33.34 Lysis2.seek 950uL @12:07:33.40 Lysis1.seek 950uL @12:07:54.32 delay 5 seconds @12:07:59.34 Handoff.select SPR @12:07:59.53 SPR.command "start 2 14" @12:08:00.93 SPR stabilized @12:08:01.14 delay 10 seconds @12:08:11.16 SPR.command "start 2 0" @12:08:14.30 Waiting up to 11:00 for SPR to enter state 1 @12:10:11.96 SPR.command "hold_on" @12:10:13.87 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @12:10:14.49 Lysis2.seek 500uL @12:10:14.60 Lysis1.seek 500uL @12:11:16.78 delay 15 seconds @12:11:31.80 SPR.command "hold_off" @12:11:34.80 Waiting up to 1:00:00 for SPR to enter state 7 @12:27:49.42 SPRsummary:15.6RIU,27.3RIU,32.4RIU,12.1RIU after 30285s @12:30:24.42 Session Ended @12:42:06.46 delay 10 seconds @12:42:16.49 SPR.command "start 2 0" @12:42:19.18 Waiting up to 11:00 for SPR to enter state 1 @12:44:16.84 SPR.command "hold_on" @12:44:18.23 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:44:18.98 Lysis2.seek 275uL @12:44:19.05 Lysis1.seek 275uL @12:44:50.25 delay 15 seconds @12:45:05.27 SPR.command "hold_off" @12:45:08.49 Waiting up to 1:00:00 for SPR to enter state 7 @13:15:41.09 delay 10 seconds @13:15:51.11 SPR.command "start 2 0" @13:15:53.81 Waiting up to 11:00 for SPR to enter state 1 @13:17:51.47 SPR.command "hold_on" @13:17:52.86 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:17:53.56 Lysis2.seek 50uL @13:17:53.63 Lysis1.seek 50uL @13:18:24.84 delay 15 seconds @13:18:39.86 SPR.command "hold_off" @13:18:43.63 Waiting up to 1:00:00 for SPR to enter state 7 @13:49:16.53 SPR analysis completed @13:49:31.60 Ended log spr24aug2111h.spr.bz2 @13:49:31.63 SPR.command "stats_off" @13:51:57.58 -> runStandard # @13:51:57.79 Selecting Cartridge 52 @13:51:58.01 Gate.power :heat,:OFF @13:51:58.03 Gate.power :cartridge,:OFF @13:51:58.15 PinPort.in :unlocking @13:51:58.24 PinPort.seek :clear @13:51:58.78 Carousel.in :default @13:51:58.91 Carousel.home.seek 53 @13:51:59.28 Archive.in :unlocking @13:51:59.43 Archive.seek :clear @13:51:59.55 Air.in :unlocking @13:51:59.71 Air.seek :clear @13:51:59.81 Lysis2.in :unlocking @13:52:00.44 Lysis2.seek :clear @13:52:04.91 Lysis1.in :unlocking @13:52:05.07 Lysis1.seek :clear @13:52:05.14 Plunge.in :unlocking @13:52:05.28 Plunge.seek :clear @13:52:09.55 Carousel.seek 52 @13:52:10.12 Lysis1.in :fast @13:52:10.20 Lysis1.seek :locked @13:52:12.87 Plunge.in :default @13:52:12.94 Plunge.seek :locked @13:52:13.13 Air.in :fast @13:52:13.20 Air.seek :locked @13:52:14.19 Carousel.reconfigure :locked @13:52:14.37 Powering cartridge bus @13:52:17.03 Gate.setIR 1750,5000,0,6 @13:52:17.16 Gate.setIR 0,5000,0,6 @13:52:17.25 PuckHeater.configure :default @13:52:17.34 Gate.power :heat,:ON,limit to 5000mA @13:52:17.95 Mounted lysis Cartridge @13:52:18.01 WARNING: Using default puck temperature sensor calibration @13:52:18.03 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:52:18.36 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 52" @13:52:18.87 SPR.command "stats_on" @13:52:19.59 SPR.command "start 0 1" @13:52:20.30 SPR.start /var/log/esp/spr24aug2113h.spr.bz2 @13:52:22.34 Waiting for baseline SPR stabilization @13:52:22.36 Handoff.select :waste @13:52:22.63 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:52:29.27 Syringe tare is 52% of full scale at 26C (drifted 0%) @13:52:29.33 PinPort.in :locking @13:52:29.46 PinPort.seek :locked @13:52:30.04 Archive.in :fast @13:52:30.19 Lysis2.in :fast @13:52:30.24 Lysis1.seek 1100uL @13:52:30.30 Archive.seek :full @13:52:30.51 Lysis2.seek 1100uL @13:52:32.55 upsync @13:52:34.88 delayUntil "14:02:21.90" @14:02:21.92 Lysis2.in :DAmix @14:02:22.06 Lysis1.in :DAmix @14:02:22.70 Lysis2.seek 950uL @14:02:22.79 Lysis1.seek 950uL @14:02:43.68 delay 5 seconds @14:02:48.70 Handoff.select SPR @14:02:48.90 SPR.command "start 2 14" @14:02:49.95 SPR stabilized @14:02:50.13 delay 10 seconds @14:03:00.15 SPR.command "start 2 0" @14:03:02.81 Waiting up to 11:00 for SPR to enter state 1 @14:05:00.47 SPR.command "hold_on" @14:05:01.85 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @14:05:02.57 Lysis2.seek 500uL @14:05:02.63 Lysis1.seek 500uL @14:06:04.86 delay 15 seconds @14:06:19.89 SPR.command "hold_off" @14:06:23.84 Waiting up to 1:00:00 for SPR to enter state 7 @14:22:39.84 SPRsummary:-0.763RIU,45.2RIU,59.6RIU,1.46RIU after 37376s @14:36:56.92 delay 10 seconds @14:37:06.94 SPR.command "start 2 0" @14:37:09.60 Waiting up to 11:00 for SPR to enter state 1 @14:39:07.27 SPR.command "hold_on" @14:39:08.65 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:39:09.30 Lysis2.seek 275uL @14:39:09.35 Lysis1.seek 275uL @14:39:40.55 delay 15 seconds @14:39:55.57 SPR.command "hold_off" @14:39:58.90 Waiting up to 1:00:00 for SPR to enter state 7 @15:10:31.50 delay 10 seconds @15:10:41.52 SPR.command "start 2 0" @15:10:44.22 Waiting up to 11:00 for SPR to enter state 1 @15:12:41.89 SPR.command "hold_on" @15:12:43.27 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @15:12:43.99 Lysis2.seek 50uL @15:12:44.06 Lysis1.seek 50uL @15:13:15.25 delay 15 seconds @15:13:30.27 SPR.command "hold_off" @15:13:33.52 Waiting up to 1:00:00 for SPR to enter state 7 @15:44:06.00 SPR analysis completed @15:44:21.99 Ended log spr24aug2113h.spr.bz2 @15:44:22.01 SPR.command "stats_off" @15:55:37.13 -> runStandard @15:55:37.32 Selecting Cartridge 51 # @15:55:37.58 Gate.power :heat,:OFF @15:55:37.60 Gate.power :cartridge,:OFF @15:55:37.72 PinPort.in :unlocking @15:55:37.80 PinPort.seek :clear @15:55:38.29 Carousel.in :default @15:55:38.45 Carousel.home.seek 52 @15:55:38.85 Archive.in :unlocking @15:55:39.01 Archive.seek :clear @15:55:39.15 Air.in :unlocking @15:55:39.29 Air.seek :clear @15:55:39.44 Lysis2.in :unlocking @15:55:39.55 Lysis2.seek :clear @15:55:44.00 Lysis1.in :unlocking @15:55:44.58 Lysis1.seek :clear @15:55:44.81 Plunge.in :unlocking @15:55:44.90 Plunge.seek :clear @15:55:49.05 Carousel.seek 51 @15:55:49.61 Lysis1.in :fast @15:55:49.70 Lysis1.seek :locked @15:55:52.35 Plunge.in :default @15:55:52.43 Plunge.seek :locked @15:55:52.61 Air.in :fast @15:55:52.70 Air.seek :locked @15:55:53.72 Carousel.reconfigure :locked @15:55:53.90 Powering cartridge bus @15:55:56.59 Gate.setIR 1750,5000,0,6 @15:55:56.73 Gate.setIR 0,5000,0,6 @15:55:56.84 PuckHeater.configure :default @15:55:56.93 Gate.power :heat,:ON,limit to 5000mA @15:55:57.56 Mounted lysis Cartridge @15:55:57.62 WARNING: Using default puck temperature sensor calibration @15:55:57.64 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:55:58.01 GoodNews.email "After filtering ml",Subject: "Processing microSPR_phyto cartridge in slot 51" @15:55:58.48 SPR.command "stats_on" @15:55:58.84 SPR.command "start 0 1" @15:55:59.49 SPR.start /var/log/esp/spr24aug2115h.spr.bz2 @15:56:01.51 Waiting for baseline SPR stabilization @15:56:01.52 Handoff.select :waste @15:56:01.83 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:56:08.48 Syringe tare is 52% of full scale at 26C (drifted 0%) @15:56:09.16 upsync @15:56:09.22 PinPort.in :locking @15:56:09.32 PinPort.seek :locked @15:56:09.84 Archive.in :fast @15:56:09.93 Lysis2.in :fast @15:56:10.01 Lysis1.seek 1100uL @15:56:10.06 Archive.seek :full @15:56:10.31 Lysis2.seek 1100uL @15:56:14.57 delayUntil "16:06:01.05" @16:06:01.07 Lysis2.in :DAmix @16:06:01.22 Lysis1.in :DAmix @16:06:01.80 Lysis2.seek 950uL @16:06:01.87 Lysis1.seek 950uL @16:06:22.79 delay 5 seconds @16:06:27.81 Handoff.select SPR @16:06:27.97 SPR.command "start 2 14" @16:06:28.57 SPR stabilized @16:06:28.79 delay 10 seconds @16:06:38.81 SPR.command "start 2 0" @16:06:41.94 Waiting up to 11:00 for SPR to enter state 1 @16:08:39.61 SPR.command "hold_on" @16:08:41.00 SPR Dilution 1 of 3 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @16:08:41.67 Lysis2.seek 500uL @16:08:41.75 Lysis1.seek 500uL @16:09:43.91 delay 15 seconds @16:09:58.95 SPR.command "hold_off" @16:10:01.51 Waiting up to 1:00:00 for SPR to enter state 7 @16:26:17.08 SPRsummary:6.59RIU,15.3RIU,9.53RIU,5.99RIU after 44464s @16:40:34.11 delay 10 seconds @16:40:44.13 SPR.command "start 2 0" @16:40:46.83 Waiting up to 11:00 for SPR to enter state 1 @16:42:44.50 SPR.command "hold_on" @16:42:46.41 SPR Dilution 2 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:42:47.13 Lysis2.seek 275uL @16:42:47.21 Lysis1.seek 275uL @16:43:18.39 delay 15 seconds @16:43:33.41 SPR.command "hold_off" @16:43:36.14 Waiting up to 1:00:00 for SPR to enter state 7 @17:14:07.79 delay 10 seconds @17:14:17.82 SPR.command "start 2 0" @17:14:20.51 Waiting up to 11:00 for SPR to enter state 1 @17:16:18.18 SPR.command "hold_on" @17:16:19.56 SPR Dilution 3 of 3 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @17:16:20.27 Lysis2.seek 50uL @17:16:20.34 Lysis1.seek 50uL @17:16:51.52 delay 15 seconds @17:17:06.55 SPR.command "hold_off" @17:17:09.82 Waiting up to 1:00:00 for SPR to enter state 7 @17:47:42.29 SPR analysis completed @17:47:58.30 Ended log spr24aug2115h.spr.bz2 @17:47:58.33 SPR.command "stats_off" @17:51:15.80 -> slot {[24,22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2023h.spr.bz2}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2115h.spr.bz2}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2113h.spr.bz2}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2111h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2110h.spr.bz2}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2107h.spr.bz2}, 56 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2104h.spr.bz2}, 57 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2102h.spr.bz2}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2020h.spr.bz2}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr24aug2018h#2.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @17:52:10.31 -> PhytoSampler.xray @17:52:22.31 -> PhytoSampler.source=nil @17:53:16.66 -> PhytoSampler.goalVolume=200 200 @17:53:48.95 -> PhytoSampler.xray @17:55:12.22 -> fire lysis # @17:55:12.45 Selecting Cartridge 24 @17:55:12.67 Gate.power :heat,:OFF @17:55:12.69 Gate.power :cartridge,:OFF @17:55:12.86 PinPort.in :unlocking @17:55:12.93 PinPort.seek :clear @17:55:13.44 Carousel.in :default @17:55:13.59 Carousel.home.seek 51 @17:55:13.99 Archive.in :unlocking @17:55:14.17 Archive.seek :clear @17:55:14.24 Air.in :unlocking @17:55:14.45 Air.seek :clear @17:55:14.62 Lysis2.in :unlocking @17:55:14.72 Lysis2.seek :clear @17:55:19.23 Lysis1.in :unlocking @17:55:19.87 Lysis1.seek :clear @17:55:20.00 Plunge.in :unlocking @17:55:20.09 Plunge.seek :clear @17:55:24.27 Carousel.seek 24 @17:55:30.12 Lysis1.in :fast @17:55:30.21 Lysis1.seek :locked @17:55:32.89 Plunge.in :default @17:55:33.05 Plunge.seek :locked @17:55:33.17 Air.in :fast @17:55:33.26 Air.seek :locked @17:55:34.25 Carousel.reconfigure :locked @17:55:34.44 Powering cartridge bus @17:55:37.15 Gate.setIR 1750,5000,0,6 @17:55:37.28 Gate.setIR 0,5000,0,6 @17:55:37.37 PuckHeater.configure :default @17:55:37.46 Gate.power :heat,:ON,limit to 5000mA @17:55:38.16 Mounted lysis Cartridge board#Q05076 @17:55:38.21 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:55:38.26 GoodNews.email "PhytoSampler filtering up to 200 ml",Subject: "Sampling microSPR_phyto cartridge in slot 24" @17:55:38.63 Preparing to filter cartridge 24 @17:55:38.93 Air.in :default @17:55:39.10 Air.seek :sealed @17:55:39.21 Priming sample loop w/150ml, bypass w/50ml @17:55:39.24 SamplePump.setPosition! 0ml @17:55:39.32 Bypass.open @17:55:40.84 Intake.open @17:55:41.69 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:55:44.32 Vacuum tare is 53% of full scale at 26C (drifted 2%) @17:55:44.54 Abs.in :default @17:55:44.63 Abs.hold @17:55:45.77 SamplePump.in :start @17:55:45.87 SamplePump.seek 50ml,3:50 @17:55:46.46 SamplePump.configure :prime @17:55:48.87 upsync @17:56:47.25 Abs.stop @17:56:47.34 SamplePump.setPosition! 0ml @17:56:47.41 Exhaust.open @17:56:48.82 Bypass.close @17:56:50.38 Abs.in :default @17:56:50.48 Abs.hold @17:56:50.84 SamplePump.in :start @17:56:50.93 SamplePump.seek 150ml,10:30 @17:56:51.53 SamplePump.configure :prime @17:59:54.42 Abs.stop @17:59:54.52 Bypass.open @17:59:55.24 Intake.close @17:59:56.03 Toroid.in :hold @17:59:56.12 Toroid.seek :crack @17:59:57.69 Toroid.seek :cartridge @17:59:58.83 Toroid.in :default @17:59:58.92 Toroid.seek :clear @18:00:01.03 Pressurizing cartridge to 26psi above 15.9psia @18:00:01.04 SamplePump.setPosition! 0ml @18:00:01.14 Intake.open @18:00:01.83 Bypass.close @18:00:02.55 Exhaust.close @18:00:03.36 Abs.hold @18:00:03.63 SamplePump.in :charge @18:00:03.73 SamplePump.seek 2.3ml,42 seconds @18:00:18.81 Abs.stop @18:00:18.90 Intake.close @18:00:19.69 Pressurized cartridge with 1.023ml to 26.0psi @18:00:19.71 Isolated @39.6psia (23.7psi) @18:00:19.74 Verifying cartridge holds at least 0.65..0.85 of 39.6psia @18:00:19.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:00:24.41 Cartridge pressure fell 2% to 38.8psia @18:00:24.54 Sampling 200.0ml @18:00:24.56 SamplePump.setPosition! 0ml @18:00:24.65 Exhaust.open @18:00:25.80 Intake.open @18:00:26.80 Abs.in :default @18:00:26.89 Abs.hold @18:00:27.23 SamplePump.in :start @18:00:27.32 SamplePump.seek 200ml,17:10 @18:00:27.91 SamplePump.configure :sample @18:00:30.33 Cartridge 24 filtering 2/200ml at 0.77ml/s with 24.5psi vacuum @18:01:30.39 Cartridge 24 filtering 45/200ml at 0.71ml/s with 21.1psi vacuum @18:02:30.39 Cartridge 24 filtering 87/200ml at 0.71ml/s with 28.6psi vacuum @18:03:30.39 Cartridge 24 filtering 129/200ml at 0.70ml/s with 21.1psi vacuum @18:04:30.39 Cartridge 24 filtering 171/200ml at 0.70ml/s with 25.5psi vacuum @18:05:12.52 Abs.stop @18:05:12.78 Intake.close @18:05:12.95 Exhaust.close @18:05:13.01 Handoff.select :close @18:05:13.29 Toroid.in :hold @18:05:13.37 Toroid.seek :bypass @18:05:15.03 Toroid.in :default @18:05:15.13 Toroid.seek :clear @18:05:16.80 Sampled 200.0ml @18:05:17.26 GoodNews.email "After filtering 200.0ml",Subject: "Processing microSPR_phyto cartridge in slot 24" @18:05:17.81 SPR.command "stats_on" @18:05:18.38 SPR.command "start 0 1" @18:05:19.07 SPR.start /var/log/esp/spr24aug2118h.spr.bz2 @18:05:21.12 Handoff.select :waste @18:05:21.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:05:22.77 upsync @18:05:27.92 Syringe tare is 52% of full scale at 26C (drifted 1%) @18:05:27.95 PinPort.in :locking @18:05:28.06 PinPort.seek :locked @18:05:28.58 Archive.in :fast @18:05:28.69 Lysis2.in :fast @18:05:28.80 Lysis1.seek 1350uL @18:05:28.84 Archive.seek :full @18:05:29.05 Lysis2.seek 950uL @18:05:33.86 Plunge.seek :reagent @18:05:34.93 Evacuating puck with filter bubble point of 24psi @18:05:34.96 PinPort.seek :locked @18:05:35.11 Handoff.select :waste @18:05:35.16 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:05:41.79 Air syringe pumping up to 1 strokes to reach 8psi @18:05:41.80 Air stroke #1 of 1 @18:05:41.96 Air.in :pressurize @18:05:42.04 Air.seek :pressurized @18:05:47.68 1.2psi after pressurize @18:05:47.71 Air.in :compress @18:05:47.82 Air.seek :evac @18:05:50.86 2.2psi after compress @18:06:08.48 0.2psi after compress (stable) @18:06:08.50 Air.in :default @18:06:08.62 Air.hold @18:06:08.92 at 0.1psi Opened to waste @18:06:08.93 Handoff.select :blocked @18:06:09.12 Archive syringe pumping up to 8 strokes to reach 24psi @18:06:09.14 Archive stroke #1 of 8 @18:06:09.27 Archive.in :pressurize @18:06:09.34 Archive.seek :pressurized @18:06:21.79 1.0psi after pressurize @18:06:27.39 0.3psi after pressurize (stable) @18:06:27.41 Archive.in :compress @18:06:27.52 Archive.seek :empty @18:06:43.26 14.4psi after compress @18:06:53.85 13.5psi after compress (stable) @18:06:53.89 Archive.in :gulp @18:06:53.99 Archive.seek :topVent @18:07:00.32 14.4psi after gulp @18:07:05.91 13.7psi after gulp (stable) @18:07:05.92 Archive stroke #2 of 8 @18:07:06.06 Archive.in :pressurize @18:07:06.69 Archive.seek :pressurized @18:07:19.25 14.3psi after pressurize @18:07:24.84 13.5psi after pressurize (stable) @18:07:24.87 Archive.in :compress @18:07:24.98 Archive.seek :empty @18:07:37.98 Reached 24.3 of 24.0psi after pumping 3401uL (1.97 strokes) @18:07:38.00 Archive.in :default @18:07:38.10 Archive.hold @18:07:38.20 Handoff.select :waste @18:07:38.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:07:51.99 delay 1:00 @18:08:52.03 Handoff.select :blocked @18:08:52.19 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @18:09:14.84 Handoff.select :waste @18:09:15.00 0.7psi at evacEnd @18:09:15.02 Handoff.select :blocked @18:09:15.22 Archive.in :fast @18:09:15.34 Archive.seek :topVent @18:09:22.33 Waiting up to 3:00 for Syringe to stabilize +/-0.30psi @18:09:28.92 Stable @0.5psi after Handoff blocked @18:09:28.95 Archive syringe pumping up to 8 strokes to reach 25psi @18:09:28.98 Archive stroke #1 of 8 @18:09:29.12 Archive.in :pressurize @18:09:29.21 Archive.seek :pressurized @18:09:41.67 1.5psi after pressurize @18:09:41.72 Archive.in :compress @18:09:41.85 Archive.seek :empty @18:09:57.59 16.2psi after compress @18:10:08.19 14.7psi after compress (stable) @18:10:08.25 Archive.in :gulp @18:10:08.36 Archive.seek :topVent @18:10:14.69 15.6psi after gulp @18:10:20.80 14.7psi after gulp (stable) @18:10:20.91 Archive stroke #2 of 8 @18:10:21.07 Archive.in :pressurize @18:10:21.16 Archive.seek :pressurized @18:10:33.61 15.3psi after pressurize @18:10:33.65 Archive.in :compress @18:10:33.79 Archive.seek :empty @18:10:46.46 Reached 24.8 of 24.5psi after pumping 3394uL (1.96 strokes) @18:10:46.48 Archive.in :default @18:10:46.59 Archive.hold @18:10:46.69 Handoff.select :waste @18:10:46.88 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:10:53.98 delay 1:00 @18:11:54.00 Handoff.select :blocked @18:11:54.18 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @18:12:17.31 Handoff.select :waste @18:12:17.56 0.5psi at evacEnd @18:12:17.58 Air.seek :fullVent @18:12:18.95 Archive.in :fast @18:12:19.05 Archive.seek :topVent @18:12:26.00 Seawater evacuated, Pressure above/below filter released. @18:12:26.12 Lysis1.in :slow @18:12:26.21 Lysis1.seek 1050uL @18:12:55.86 delay 5 seconds @18:13:00.88 Handoff.select :blocked @18:13:01.12 Lysis1.seek 450uL @18:14:00.26 delay 3 seconds @18:14:03.35 Air.in mix with 1.57127 parts Lysis1 @18:14:04.57 Air.seek :evac @18:14:04.70 Lysis1.seek 300uL @18:14:19.68 Lysis1.seek :empty @18:14:49.44 Lysis buffer delivered @21.2psi @18:14:49.46 Polling Can every 2:00 @18:14:50.04 PuckHeater.configure :default #ambient 26.4C @18:14:50.15 PuckHeater.seek 79.6C @18:15:41.94 PuckHeater.hold 2:00,79.6C @18:17:41.77 Polling Can every 2:00 @18:17:42.32 PuckHeater.configure :default #ambient 26.5C @18:17:42.40 PuckHeater.hold 30 seconds,78.5C @18:18:12.55 Polling Can every 2:00 @18:18:13.10 PuckHeater.configure :default #ambient 26.5C @18:18:13.19 PuckHeater.hold 30 seconds,78C @18:18:43.35 Polling Can every 2:00 @18:18:43.91 PuckHeater.configure :default #ambient 26.5C @18:18:44.01 PuckHeater.hold 10:00,77.5C @18:28:42.61 Lysis complete. Syringe pressure @19.5psi @18:28:42.67 Archive syringe pumping up to 5 strokes to reach 40psi @18:28:42.68 Archive stroke #1 of 5 @18:28:42.84 Archive.in :pressurize @18:28:42.93 Archive.seek :pressurized @18:28:55.38 19.6psi after pressurize @18:29:00.98 18.9psi after pressurize (stable) @18:29:01.00 Archive.in :compress @18:29:01.11 Archive.seek :empty @18:29:10.96 Reached 40.8 of 40.0psi after pumping 1604uL (0.93 strokes) @18:29:10.98 Archive.in :default @18:29:11.08 Archive.hold @18:29:11.19 delay 2 seconds @18:29:13.21 Archive.in :fast @18:29:13.30 Archive.seek :full @18:29:20.63 Lysis1.seek 138uL @18:29:34.35 delay 1 second @18:29:35.41 Archive.seek :full @18:29:35.49 Lysis1.seek :empty @18:29:49.28 Handoff.select :blocked @18:29:49.43 Initial air pressure @43.5psi @18:29:49.55 Lysis1.in :extract @18:29:49.67 Lysis1.seek 600uL,nil @18:29:52.79 Archive syringe pumping up to 6 strokes to reach 25psi @18:29:52.80 Archive stroke #1 of 6 @18:29:52.95 Archive.in :pressurize @18:29:53.07 Archive.seek :pressurized @18:30:05.54 13.6psi after pressurize @18:30:11.63 12.8psi after pressurize (stable) @18:30:11.66 Archive.in :compress @18:30:11.79 Archive.seek :empty @18:30:19.32 Archive.in :default @18:30:19.39 Archive.hold @18:30:19.59 Initial 600uL lysate @19.5psi @18:30:19.62 Handoff.select :blocked @18:30:19.75 Initial air pressure @18.8psi @18:30:19.89 Lysis1.seek 780uL,nil @18:30:21.27 Archive syringe pumping up to 6 strokes to reach 17psi @18:30:21.28 Archive stroke #1 of 6 @18:30:21.42 Archive.in :compress @18:30:21.54 Archive.seek :empty @18:30:22.22 Archive.in :default @18:30:22.28 Archive.hold @18:30:22.58 Recovered 780uL lysate @17.5psi @18:30:22.63 Air.in :halfSpeed @18:30:22.77 Air.seek :fullVent @18:30:24.84 Puck Top Vented, bottom @21.2psi @18:30:24.85 Handoff.select :waste @18:30:25.02 delay 5 seconds @18:30:30.10 Handoff open to waste, bottom @0.4psi @18:30:30.13 Air.in :default @18:30:30.26 Air.seek :evac @18:30:31.62 Lysis2.in :DAmix @18:30:31.77 Lysis1.in :DAmix @18:30:32.54 Lysis2.seek 800uL @18:30:32.65 Lysis1.seek 630uL @18:30:53.51 delay 5 seconds @18:30:58.53 Handoff.select SPR @18:30:58.78 SPR.command "start 2 14" @18:30:59.97 SPR stabilized @18:31:00.17 delay 10 seconds @18:31:10.19 SPR.command "start 2 0" @18:31:13.87 Waiting up to 11:00 for SPR to enter state 1 @18:31:26.50 Session Begun @18:31:30.26 -> showlog -300 @18:31:58.76 -> showlog -1000 @18:33:11.95 SPR.command "hold_on" @18:33:13.35 SPR Dilution 1 of 1 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @18:33:14.14 Lysis2.seek 350uL @18:33:14.24 Lysis1.seek 180uL @18:34:16.41 delay 15 seconds @18:34:31.43 SPR.command "hold_off" @18:34:34.80 Waiting up to 1:00:00 for SPR to enter state 7 @18:50:50.38 SPRsummary:4.58RIU,5.89RIU,7.31RIU,5.79RIU,high,high,high after 52517s @19:05:07.29 SPR analysis completed @19:05:16.91 Ended log spr24aug2118h.spr.bz2 @19:05:16.93 SPR.command "stats_off" @19:05:17.36 Handoff.select :close @19:05:17.55 Gate.power :heat,:OFF @19:05:17.57 Gate.power :cartridge,:OFF @19:05:17.72 PinPort.in :unlocking @19:05:17.81 PinPort.seek :clear @19:05:18.32 Carousel.in :default @19:05:18.55 Carousel.home.seek 24 @19:05:18.95 Plunge.seek :locked @19:05:19.88 Archive.in :unlocking @19:05:20.06 Archive.seek :clear @19:05:20.13 Air.in :unlocking @19:05:20.37 Air.seek :clear @19:05:20.56 Lysis1.in :unlocking @19:05:21.24 Lysis1.seek :clear @19:05:21.44 Lysis2.in :unlocking @19:05:21.54 Lysis2.seek :clear @19:05:25.58 Gate.power :analytic1,:OFF @19:05:25.61 Gate.power :motor,:OFF @19:05:25.67 Gate.power :core,:OFF @19:05:25.71 Gate.power :main,:OFF @19:05:25.74 Safely stopped and ready to power off @19:22:08.94 -> Cmd.stop @19:22:09.02 ready to power off :STOPPED @13:25:39.50UTC22-Aug-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:25:43.23 >BREAK< ESP3 Gateway v1.43 for core bus @13:25:43.26 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:25:47.89 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @13:25:49.03 Sea pressure (Abs) tare is 6% of full scale @13:26:04.81 Polling Can every 2:00 @13:26:04.88 Waiting for espclients... @13:26:05.66 Session Begun @13:26:09.92 -> sot :sot @13:26:12.50 -> slot {[22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 200, lysed: 780, spr: "spr24aug2118h.spr.bz2"}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2023h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2115h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2113h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2111h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2110h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2107h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2104h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2102h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2020h.spr.bz2"}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2018h#2.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @13:26:42.51 -> State: :processed, @13:26:42.54 SyntaxError in bill -- compile error bill:3: syntax error, unexpected tLABEL State: :processed, ^ @13:26:43.08 -> lysed: 1100, @13:26:43.12 SyntaxError in bill -- compile error bill:4: syntax error, unexpected tLABEL lysed: 1100, ^ @13:26:43.20 -> lysed2: 1100, @13:26:43.23 SyntaxError in bill -- compile error bill:5: syntax error, unexpected tLABEL lysed2: 1100, ^ @13:26:43.35 -> spr: "spr24aug2020h.spr.bz2"}, @13:26:43.39 SyntaxError in bill -- compile error bill:6: syntax error, unexpected tLABEL spr: "spr24aug2020h.spr.bz2"}, ^ bill:6: syntax error, unexpected '}', expecting $end spr: "spr24aug2020h.spr.bz2"}, ^ @13:26:43.49 -> 59 => { Type: :microSPR_phyto, @13:26:43.51 SyntaxError in bill -- compile error bill:7: syntax error, unexpected tASSOC, expecting $end 59 => { Type: :microSPR_phyto, ^ @13:26:43.61 -> State: :processed, @13:26:43.64 SyntaxError in bill -- compile error bill:8: syntax error, unexpected tLABEL State: :processed, ^ @13:26:43.73 -> lysed: 1100, @13:26:43.77 SyntaxError in bill -- compile error bill:9: syntax error, unexpected tLABEL lysed: 1100, ^ @13:26:43.84 -> lysed2: 1100, @13:26:43.89 SyntaxError in bill -- compile error bill:10: syntax error, unexpected tLABEL lysed2: 1100, ^ @13:26:44.05 -> spr: "spr24aug2018h#2.spr.bz2"}, @13:26:44.08 SyntaxError in bill -- compile error bill:11: syntax error, unexpected tLABEL spr: "spr24aug2018h#2.spr.bz2"}, ^ bill:11: syntax error, unexpected '}', expecting $end spr: "spr24aug2018h#2.spr.bz2"}, ^ @13:26:44.22 -> 60 => { Type: :microSPR_phyto, @13:26:44.25 SyntaxError in bill -- compile error bill:12: syntax error, unexpected tASSOC, expecting $end 60 => { Type: :microSPR_phyto, ^ @13:26:44.36 -> State: :processed, @13:26:44.38 SyntaxError in bill -- compile error bill:13: syntax error, unexpected tLABEL State: :processed, ^ @13:26:44.47 -> filtered: 50, @13:26:44.52 SyntaxError in bill -- compile error bill:14: syntax error, unexpected tLABEL filtered: 50, ^ @13:26:44.60 -> lysed: 825, @13:26:44.63 SyntaxError in bill -- compile error bill:15: syntax error, unexpected tLABEL lysed: 825, ^ @13:26:44.75 -> spr: "spr24aug0119h.spr.bz2"}} @13:26:44.77 SyntaxError in bill -- compile error bill:16: syntax error, unexpected tLABEL spr: "spr24aug0119h.spr.bz2"}} ^ bill:16: syntax error, unexpected '}', expecting $end spr: "spr24aug0119h.spr.bz2"}} ^ @13:26:44.86 -> bill@honu:003-> @13:26:44.89 SyntaxError in bill -- compile error bill:17: syntax error, unexpected ':', expecting $end bill@honu:003-> ^ @13:26:44.99 -> State: :processed, @13:26:45.02 SyntaxError in bill -- compile error bill:18: syntax error, unexpected tLABEL State: :processed, ^ @13:26:45.10 -> lysed: 1100, @13:26:45.15 SyntaxError in bill -- compile error bill:19: syntax error, unexpected tLABEL lysed: 1100, ^ @13:26:45.22 -> lysed2: 1100, @13:26:45.26 SyntaxError in bill -- compile error bill:20: syntax error, unexpected tLABEL lysed2: 1100, ^ @13:26:45.37 -> spr: "spr24aug2020h.spr.bz2"}, @13:26:45.39 SyntaxError in bill -- compile error bill:21: syntax error, unexpected tLABEL spr: "spr24aug2020h.spr.bz2"}, ^ bill:21: syntax error, unexpected '}', expecting $end spr: "spr24aug2020h.spr.bz2"}, ^ @13:26:45.53 -> 59 => { Type: :microSPR_phyto, @13:26:45.56 SyntaxError in bill -- compile error bill:22: syntax error, unexpected tASSOC, expecting $end 59 => { Type: :microSPR_phyto, ^ @13:26:45.68 -> State: :processed, @13:26:45.70 SyntaxError in bill -- compile error bill:23: syntax error, unexpected tLABEL State: :processed, ^ @13:26:45.79 -> lysed: 1100, @13:26:45.82 SyntaxError in bill -- compile error bill:24: syntax error, unexpected tLABEL lysed: 1100, ^ @13:26:45.91 -> lysed2: 1100, @13:26:45.97 SyntaxError in bill -- compile error bill:25: syntax error, unexpected tLABEL lysed2: 1100, ^ @13:26:46.08 -> spr: "spr24aug2018h#2.spr.bz2"}, @13:26:46.12 SyntaxError in bill -- compile error bill:26: syntax error, unexpected tLABEL spr: "spr24aug2018h#2.spr.bz2"}, ^ bill:26: syntax error, unexpected '}', expecting $end spr: "spr24aug2018h#2.spr.bz2"}, ^ @13:26:46.24 -> 60 => { Type: :microSPR_phyto, @13:26:46.28 SyntaxError in bill -- compile error bill:27: syntax error, unexpected tASSOC, expecting $end 60 => { Type: :microSPR_phyto, ^ @13:26:46.36 -> State: :processed, @13:26:46.39 SyntaxError in bill -- compile error bill:28: syntax error, unexpected tLABEL State: :processed, ^ @13:26:46.49 -> filtered: 50, @13:26:46.53 SyntaxError in bill -- compile error bill:29: syntax error, unexpected tLABEL filtered: 50, ^ @13:26:46.60 -> lysed: 825, @13:26:46.63 SyntaxError in bill -- compile error bill:30: syntax error, unexpected tLABEL lysed: 825, ^ @13:26:46.77 -> spr: "spr24aug0119h.spr.bz2"}} @13:26:46.81 SyntaxError in bill -- compile error bill:31: syntax error, unexpected tLABEL spr: "spr24aug0119h.spr.bz2"}} ^ bill:31: syntax error, unexpected '}', expecting $end spr: "spr24aug0119h.spr.bz2"}} ^ @13:26:46.90 -> bill@honu:003-> @13:26:46.94 SyntaxError in bill -- compile error bill:32: syntax error, unexpected ':', expecting $end bill@honu:003-> ^ @13:27:50.70 -> SC @13:27:50.75 BusOff in bill -- core bus was not powered while reading Storage Carousel (SC)'s status @13:28:01.88 -> ESP.ready! @13:28:01.97 Gate.power :sampler,:OFF,limit to 3500mA @13:28:01.99 Gate.power :skimmer,:OFF,limit to 3500mA @13:28:02.02 Gate.power :main,:ON,limit to 5000mA @13:28:02.06 Gate.power :analytic1,:ON,limit to 4000mA @13:28:02.27 Powering core bus @13:28:05.04 Plunge.configure :default @13:28:05.15 SamplePump.configure :default @13:28:05.25 Toroid.configure :default @13:28:05.35 Carousel.configure :default @13:28:05.46 Abs.configure :default @13:28:05.56 PhaseSeparator.configure :default @13:28:05.66 Archive.configure :default @13:28:05.76 Air.configure :default @13:28:05.84 Lysis1.configure :default @13:28:06.18 Lysis2.configure :default @13:28:06.29 PinPort.configure :default @13:28:06.37 All elf controllers running firmware version 4.92 @13:28:06.41 Gate.power :motor,:ON,limit to 5000mA @13:28:09.43 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @13:28:09.46 AnalyticModule::Unknown in bill -- SPR @13:28:09.96 Toroid.home.jog 0 @13:28:10.07 Plunge.home.jog 0 @13:28:10.64 Lysis2.home.jog 0 @13:28:10.72 Air.home.jog 0 @13:28:10.75 Carousel.home.jog 0 @13:28:10.79 PinPort.home.jog 0 @13:28:10.82 Archive.home.jog 0 @13:28:10.87 Lysis1.home.jog 0 @13:28:11.50 Plunge.in :homing @13:28:11.68 Toroid.in :homing @13:28:11.85 Toroid.jog 627 @13:28:11.88 Plunge.jog 1597 @13:28:12.18 Lysis1.in :homing @13:28:12.25 Air.in :homing @13:28:12.30 Lysis2.in :homing @13:28:12.34 PinPort.in :homing @13:28:12.42 Archive.in :homing @13:28:12.56 Lysis1.jog 1399 @13:28:12.64 Air.jog 1378 @13:28:12.71 PinPort.jog -110 @13:28:12.75 Lysis2.jog 1399 @13:28:12.81 Archive.jog 1399 @13:28:13.16 PinPort.setRawPosition! 0 @13:28:13.23 PinPort.seek :home @13:28:13.37 Plunge.setRawPosition! 50 @13:28:13.40 PinPort.in :default @13:28:13.51 Plunge.seek :home @13:28:13.84 Plunge.in :default @13:28:13.98 Lysis1.setRawPosition! 50 @13:28:14.07 Lysis1.seek :home @13:28:14.20 Lysis2.setRawPosition! 50 @13:28:14.25 Air.setRawPosition! 50 @13:28:14.28 Toroid.setRawPosition! 0 @13:28:14.88 Air.seek :home @13:28:14.94 Lysis2.seek :home @13:28:14.98 Toroid.seek :home @13:28:15.35 Archive.setRawPosition! 50 @13:28:15.40 Toroid.in :default @13:28:15.45 Lysis1.in :default @13:28:15.64 Archive.seek :home @13:28:15.78 Air.in :default @13:28:15.80 Lysis2.in :default @13:28:16.02 Archive.in :default @13:28:16.39 Toroid.seek :clear @13:28:18.05 Carousel.in :default @13:28:18.21 Carousel.home.seek 24 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 24 Abs = Absolute Pressure (Leak) Sensor @19psia PhaseSeparator = Intake Head Pressure @2.18psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:28:23.93 -> SC Storage Carousel (SC) at 24 @13:29:39.97 -> ESP.shutdown @13:29:40.17 Gate.power :analytic1,:OFF @13:29:40.19 Gate.power :motor,:OFF @13:29:40.23 Gate.power :core,:OFF @13:29:40.28 Gate.power :main,:OFF [:analytic1,:motor,:core,:main] @14:33:45.66 -> require 'vacuumCarts' @14:33:45.71 LoadError in bill -- no such file to load -- vacuumCarts @14:33:54.19 -> require 'vacuumCart' true @14:34:01.56 -> cleanToroid @14:34:01.59 Cleans Dry SC positions in slots, begin in 10% Bleach @14:34:01.66 Gate.power :sampler,:OFF,limit to 3500mA @14:34:01.69 Gate.power :skimmer,:OFF,limit to 3500mA @14:34:01.71 Gate.power :main,:ON,limit to 5000mA @14:34:01.75 Gate.power :analytic1,:ON,limit to 4000mA @14:34:01.85 Powering core bus @14:34:04.62 Plunge.configure :default @14:34:04.73 SamplePump.configure :default @14:34:04.84 Toroid.configure :default @14:34:04.95 Carousel.configure :default @14:34:05.06 Abs.configure :default @14:34:05.17 PhaseSeparator.configure :default @14:34:05.27 Archive.configure :default @14:34:05.38 Air.configure :default @14:34:05.46 Lysis1.configure :default @14:34:05.53 Lysis2.configure :default @14:34:05.91 PinPort.configure :default @14:34:06.02 All elf controllers running firmware version 4.92 @14:34:06.05 Gate.power :motor,:ON,limit to 5000mA @14:34:09.11 AnalyticModule::Error in Probe-AMspr -- No Response to BREAK @14:34:09.14 AnalyticModule::Unknown in bill -- SPR @14:34:09.64 Plunge.home.jog 0 @14:34:09.71 Toroid.home.jog 0 @14:34:09.82 Carousel.home.jog 0 @14:34:10.52 Lysis2.home.jog 0 @14:34:10.59 Archive.home.jog 0 @14:34:10.65 Lysis1.home.jog 0 @14:34:10.69 Air.home.jog 0 @14:34:10.74 PinPort.home.jog 0 @14:34:11.40 Toroid.in :homing @14:34:11.50 Plunge.in :homing @14:34:12.26 Toroid.jog 627 @14:34:12.28 Plunge.jog 158 @14:34:12.59 PinPort.in :homing @14:34:12.63 Lysis2.in :homing @14:34:12.68 Air.in :homing @14:34:12.72 Archive.in :homing @14:34:12.81 Lysis1.in :homing @14:34:12.95 PinPort.jog -109 @14:34:13.06 Lysis2.jog 1399 @14:34:13.27 Lysis1.jog 1393 @14:34:13.34 Air.jog 1377 @14:34:13.40 Archive.jog 1399 @14:34:13.64 PinPort.setRawPosition! 0 @14:34:13.71 Plunge.setRawPosition! 50 @14:34:13.82 PinPort.seek :home @14:34:13.97 Plunge.seek :home @14:34:14.17 PinPort.in :default @14:34:14.33 Plunge.in :default @14:34:14.42 Lysis2.setRawPosition! 50 @14:34:14.52 Lysis2.seek :home @14:34:14.68 Lysis1.setRawPosition! 50 @14:34:14.71 Air.setRawPosition! 50 @14:34:14.75 Toroid.setRawPosition! 0 @14:34:14.93 Air.seek :home @14:34:15.00 Lysis1.seek :home @14:34:15.05 Toroid.seek :home @14:34:15.37 Archive.setRawPosition! 50 @14:34:15.42 Toroid.in :default @14:34:15.46 Lysis2.in :default @14:34:15.64 Archive.seek :home @14:34:15.81 Air.in :default @14:34:15.84 Lysis1.in :default @14:34:16.05 Archive.in :default @14:34:16.41 Toroid.seek :clear @14:34:18.14 Carousel.in :default @14:34:18.29 Carousel.home.seek 9 @14:34:18.76 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:34:30.24 UserAbort in bill -- User Abort!! @14:34:36.19 -> slot {[22,20,18,16,14,12,10,8,6,4,2,1] => { Type: :microSPR_phyto, State: :dry}, [35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,3] => { Type: :archiveHiBiomass_bac, State: :dry}, 5 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 24 => { Type: :microSPR_phyto, State: :processed, filtered: 200, lysed: 780, spr: "spr24aug2118h.spr.bz2"}, 26 => { Type: :microSPR_phyto, State: :processed, filtered: 75, lysed: 780, spr: "spr24aug2013h.spr.bz2"}, 28 => { Type: :microSPR_phyto, State: :processed, filtered: 175, lysed: 780, spr: "spr24aug2012h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 266.9, lysed: 780, spr: "spr24aug1919h.spr.bz2"}, 32 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 780, spr: "spr24aug1917h.spr.bz2"}, 34 => { Type: :microSPR_phyto, State: :processed, filtered: 491.2, lysed: 780, spr: "spr24aug1419h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 264.5, lysed: 780, spr: "spr24aug0919h.spr.bz2"}, [45,41,39,37] => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 175}, 38 => { Type: :microSPR_phyto, State: :processed, filtered: 541.2, lysed: 780, spr: "spr24aug0823h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 167.3, lysed: 780, spr: "spr24aug0717h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 780, spr: "spr24aug0712h.spr.bz2"}, 43 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 166.8}, 44 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 826, spr: "spr24aug0618h.spr.bz2"}, 46 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0600h.spr.bz2"}, 47 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 188.1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 25, lysed: 825, spr: "spr24aug0519h.spr.bz2"}, 49 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 528.9}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2023h.spr.bz2"}, 51 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2115h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2113h.spr.bz2"}, 53 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2111h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2110h.spr.bz2"}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2107h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2104h.spr.bz2"}, 57 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2102h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2020h.spr.bz2"}, 59 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr24aug2018h#2.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, filtered: 50, lysed: 825, spr: "spr24aug0119h.spr.bz2"}} @14:34:46.44 -> slot =>[:dry] @14:34:46.46 SyntaxError in bill -- compile error bill:41: syntax error, unexpected tASSOC, expecting $end slot =>[:dry] ^ @14:35:00.16 -> slot [60..1] =>[:dry] {60..1 => { Type: :unknown, State: :dry}} @14:35:03.41 -> slot {60..1 => { Type: :unknown, State: :dry}} @14:35:10.70 -> cleanToroid @14:35:10.72 Cleans Dry SC positions in slots, begin in 10% Bleach @14:35:11.50 Toroid.seek :clear @14:35:11.69 Carousel.in :default @14:35:11.83 Carousel.home.seek 9 @14:35:12.12 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:35:30.38 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @14:35:30.48 Priming sample loop w/100ml, bypass w/50ml @14:35:30.51 SamplePump.setPosition! 0ml @14:35:30.59 Bypass.open @14:35:32.09 Intake.open @14:35:32.90 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:35:35.52 Vacuum tare is 52% of full scale at 20C (drifted 1%) @14:35:36.16 Abs.in :default @14:35:36.23 Abs.hold @14:35:36.32 PhaseSeparator.in :full @14:35:36.39 PhaseSeparator.hold @14:35:36.75 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.05psi @14:35:43.34 SamplePump.stop @14:35:43.42 Intake.close @14:35:43.47 Bypass.close @14:35:44.29 Abs.stop @14:35:44.37 PhaseSeparator.stop @14:35:44.46 Scale::Skew::CannotTare in bill -- Intake Head Pressure sensor @31.6% scale out of usable range @14:35:57.47 -> BACsampler.source=nil @14:36:02.44 -> BACsampler.xray @14:36:32.40 -> cleanToroid @14:36:32.43 Cleans Dry SC positions in slots, begin in 10% Bleach @14:36:33.19 Toroid.seek :clear @14:36:33.41 Carousel.in :default @14:36:33.55 Carousel.home.seek 9 @14:36:33.84 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @14:49:11.16 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @14:49:11.27 Priming sample loop w/100ml, bypass w/50ml @14:49:11.29 SamplePump.setPosition! 0ml @14:49:11.37 Bypass.open @14:49:12.85 Intake.open @14:49:13.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:49:16.29 Vacuum tare is 52% of full scale at 20C (drifted 1%) @14:49:16.47 Abs.in :default @14:49:16.55 Abs.hold @14:49:16.81 SamplePump.in :start @14:49:16.90 SamplePump.seek 50ml,3:50 @14:49:17.50 SamplePump.configure :prime @14:50:18.30 Abs.stop @14:50:18.92 SamplePump.setPosition! 0ml @14:50:18.99 Exhaust.open @14:50:20.39 Bypass.close @14:50:21.91 Abs.in :default @14:50:22.01 Abs.hold @14:50:22.33 SamplePump.in :start @14:50:22.42 SamplePump.seek 100ml,7:10 @14:50:23.03 SamplePump.configure :prime @14:52:24.84 Abs.stop @14:52:24.92 Opening all dry Cartridges @14:52:24.98 Ensuring (60) cartridge(s) open @14:52:25.28 Carousel.seek 1 @14:52:27.13 Toroid.in :hold @14:52:27.22 Toroid.seek :cartridge @14:52:29.08 Toroid.in :default @14:52:29.17 Toroid.seek :clear @14:52:31.28 Carousel.seek 2 @14:52:31.71 Toroid.in :hold @14:52:31.78 Toroid.seek :cartridge @14:52:33.64 Toroid.in :default @14:52:33.73 Toroid.seek :clear @14:52:35.84 Carousel.seek 3 @14:52:36.28 Toroid.in :hold @14:52:36.37 Toroid.seek :cartridge @14:52:38.23 Toroid.in :default @14:52:38.31 Toroid.seek :clear @14:52:40.42 Carousel.seek 4 @14:52:40.85 Toroid.in :hold @14:52:40.94 Toroid.seek :cartridge @14:52:42.80 Toroid.in :default @14:52:42.89 Toroid.seek :clear @14:52:44.99 Carousel.seek 5 @14:52:45.41 Toroid.in :hold @14:52:45.50 Toroid.seek :cartridge @14:52:47.36 Toroid.in :default @14:52:47.44 Toroid.seek :clear @14:52:49.55 Carousel.seek 6 @14:52:49.98 Toroid.in :hold @14:52:50.06 Toroid.seek :cartridge @14:52:52.36 Toroid.in :default @14:52:52.45 Toroid.seek :clear @14:52:54.55 Carousel.seek 7 @14:52:54.98 Toroid.in :hold @14:52:55.06 Toroid.seek :cartridge @14:52:56.92 Toroid.in :default @14:52:57.00 Toroid.seek :clear @14:52:59.10 Carousel.seek 8 @14:52:59.53 Toroid.in :hold @14:52:59.61 Toroid.seek :cartridge @14:53:01.46 Toroid.in :default @14:53:01.60 Toroid.seek :clear @14:53:03.71 Carousel.seek 9 @14:53:04.14 Toroid.in :hold @14:53:04.21 Toroid.seek :cartridge @14:53:06.07 Toroid.in :default @14:53:06.16 Toroid.seek :clear @14:53:08.25 Carousel.seek 10 @14:53:08.68 Toroid.in :hold @14:53:08.77 Toroid.seek :cartridge @14:53:10.62 Toroid.in :default @14:53:10.70 Toroid.seek :clear @14:53:12.81 Carousel.seek 11 @14:53:13.24 Toroid.in :hold @14:53:13.32 Toroid.seek :cartridge @14:53:15.18 Toroid.in :default @14:53:15.26 Toroid.seek :clear @14:53:17.35 Carousel.seek 12 @14:53:17.78 Toroid.in :hold @14:53:17.87 Toroid.seek :cartridge @14:53:19.75 Toroid.in :default @14:53:19.84 Toroid.seek :clear @14:53:21.98 Carousel.seek 13 @14:53:22.41 Toroid.in :hold @14:53:22.50 Toroid.seek :cartridge @14:53:24.36 Toroid.in :default @14:53:24.89 Toroid.seek :clear @14:53:27.00 Carousel.seek 14 @14:53:27.43 Toroid.in :hold @14:53:27.51 Toroid.seek :cartridge @14:53:29.37 Toroid.in :default @14:53:29.46 Toroid.seek :clear @14:53:31.56 Carousel.seek 15 @14:53:31.99 Toroid.in :hold @14:53:32.07 Toroid.seek :cartridge @14:53:33.95 Toroid.in :default @14:53:34.04 Toroid.seek :clear @14:53:36.24 Carousel.seek 16 @14:53:36.67 Toroid.in :hold @14:53:36.75 Toroid.seek :cartridge @14:53:38.61 Toroid.in :default @14:53:38.70 Toroid.seek :clear @14:53:40.80 Carousel.seek 17 @14:53:41.23 Toroid.in :hold @14:53:41.32 Toroid.seek :cartridge @14:53:43.18 Toroid.in :default @14:53:43.26 Toroid.seek :clear @14:53:45.38 Carousel.seek 18 @14:53:45.80 Toroid.in :hold @14:53:45.88 Toroid.seek :cartridge @14:53:47.77 Toroid.in :default @14:53:47.86 Toroid.seek :clear @14:53:50.03 Carousel.seek 19 @14:53:50.46 Toroid.in :hold @14:53:50.55 Toroid.seek :cartridge @14:53:52.41 Toroid.in :default @14:53:52.49 Toroid.seek :clear @14:53:54.60 Carousel.seek 20 @14:53:55.03 Toroid.in :hold @14:53:55.11 Toroid.seek :cartridge @14:53:56.96 Toroid.in :default @14:53:57.05 Toroid.seek :clear @14:53:59.60 Carousel.seek 21 @14:54:00.03 Toroid.in :hold @14:54:00.11 Toroid.seek :cartridge @14:54:01.98 Toroid.in :default @14:54:02.07 Toroid.seek :clear @14:54:04.18 Carousel.seek 22 @14:54:04.61 Toroid.in :hold @14:54:04.69 Toroid.seek :cartridge @14:54:06.54 Toroid.in :default @14:54:06.63 Toroid.seek :clear @14:54:08.74 Carousel.seek 23 @14:54:09.17 Toroid.in :hold @14:54:09.25 Toroid.seek :cartridge @14:54:11.11 Toroid.in :default @14:54:11.20 Toroid.seek :clear @14:54:13.29 Carousel.seek 24 @14:54:13.72 Toroid.in :hold @14:54:13.81 Toroid.seek :cartridge @14:54:15.67 Toroid.in :default @14:54:15.75 Toroid.seek :clear @14:54:17.86 Carousel.seek 25 @14:54:18.32 Toroid.in :hold @14:54:18.40 Toroid.seek :cartridge @14:54:20.26 Toroid.in :default @14:54:20.35 Toroid.seek :clear @14:54:22.45 Carousel.seek 26 @14:54:22.88 Toroid.in :hold @14:54:22.97 Toroid.seek :cartridge @14:54:24.83 Toroid.in :default @14:54:24.92 Toroid.seek :clear @14:54:27.01 Carousel.seek 27 @14:54:27.44 Toroid.in :hold @14:54:27.52 Toroid.seek :cartridge @14:54:29.38 Toroid.in :default @14:54:29.47 Toroid.seek :clear @14:54:32.05 Carousel.seek 28 @14:54:32.48 Toroid.in :hold @14:54:32.57 Toroid.seek :cartridge @14:54:34.43 Toroid.in :default @14:54:34.52 Toroid.seek :clear @14:54:36.63 Carousel.seek 29 @14:54:37.05 Toroid.in :hold @14:54:37.14 Toroid.seek :cartridge @14:54:38.99 Toroid.in :default @14:54:39.08 Toroid.seek :clear @14:54:41.18 Carousel.seek 30 @14:54:41.61 Toroid.in :hold @14:54:41.70 Toroid.seek :cartridge @14:54:43.56 Toroid.in :default @14:54:43.65 Toroid.seek :clear @14:54:45.75 Carousel.seek 31 @14:54:46.17 Toroid.in :hold @14:54:46.25 Toroid.seek :cartridge @14:54:48.11 Toroid.in :default @14:54:48.20 Toroid.seek :clear @14:54:50.30 Carousel.seek 32 @14:54:50.73 Toroid.in :hold @14:54:50.81 Toroid.seek :cartridge @14:54:52.67 Toroid.in :default @14:54:52.76 Toroid.seek :clear @14:54:54.87 Carousel.seek 33 @14:54:55.30 Toroid.in :hold @14:54:55.39 Toroid.seek :cartridge @14:54:57.24 Toroid.in :default @14:54:57.33 Toroid.seek :clear @14:54:59.44 Carousel.seek 34 @14:54:59.87 Toroid.in :hold @14:54:59.99 Toroid.seek :cartridge @14:55:01.85 Toroid.in :default @14:55:02.00 Toroid.seek :clear @14:55:04.16 Carousel.seek 35 @14:55:05.04 Toroid.in :hold @14:55:05.13 Toroid.seek :cartridge @14:55:06.99 Toroid.in :default @14:55:07.07 Toroid.seek :clear @14:55:09.17 Carousel.seek 36 @14:55:09.57 Toroid.in :hold @14:55:09.65 Toroid.seek :cartridge @14:55:11.51 Toroid.in :default @14:55:11.60 Toroid.seek :clear @14:55:13.69 Carousel.seek 37 @14:55:14.12 Toroid.in :hold @14:55:14.20 Toroid.seek :cartridge @14:55:16.06 Toroid.in :default @14:55:16.14 Toroid.seek :clear @14:55:18.24 Carousel.seek 38 @14:55:18.67 Toroid.in :hold @14:55:18.74 Toroid.seek :cartridge @14:55:20.60 Toroid.in :default @14:55:20.68 Toroid.seek :clear @14:55:22.79 Carousel.seek 39 @14:55:23.22 Toroid.in :hold @14:55:23.31 Toroid.seek :cartridge @14:55:25.17 Toroid.in :default @14:55:25.25 Toroid.seek :clear @14:55:27.36 Carousel.seek 40 @14:55:27.80 Toroid.in :hold @14:55:27.88 Toroid.seek :cartridge @14:55:29.77 Toroid.in :default @14:55:29.86 Toroid.seek :clear @14:55:32.03 Carousel.seek 41 @14:55:32.46 Toroid.in :hold @14:55:32.55 Toroid.seek :cartridge @14:55:34.41 Toroid.in :default @14:55:34.49 Toroid.seek :clear @14:55:36.58 Carousel.seek 42 @14:55:37.01 Toroid.in :hold @14:55:37.55 Toroid.seek :cartridge @14:55:39.41 Toroid.in :default @14:55:39.50 Toroid.seek :clear @14:55:41.60 Carousel.seek 43 @14:55:42.15 Toroid.in :hold @14:55:42.25 Toroid.seek :cartridge @14:55:44.11 Toroid.in :default @14:55:44.20 Toroid.seek :clear @14:55:46.31 Carousel.seek 44 @14:55:46.74 Toroid.in :hold @14:55:46.83 Toroid.seek :cartridge @14:55:48.70 Toroid.in :default @14:55:48.78 Toroid.seek :clear @14:55:50.90 Carousel.seek 45 @14:55:51.33 Toroid.in :hold @14:55:51.40 Toroid.seek :cartridge @14:55:53.26 Toroid.in :default @14:55:53.35 Toroid.seek :clear @14:55:55.46 Carousel.seek 46 @14:55:55.91 Toroid.in :hold @14:55:56.02 Toroid.seek :cartridge @14:55:57.88 Toroid.in :default @14:55:58.00 Toroid.seek :clear @14:56:00.14 Carousel.seek 47 @14:56:00.58 Toroid.in :hold @14:56:00.67 Toroid.seek :cartridge @14:56:02.53 Toroid.in :default @14:56:02.62 Toroid.seek :clear @14:56:04.71 Carousel.seek 48 @14:56:05.13 Toroid.in :hold @14:56:05.22 Toroid.seek :cartridge @14:56:07.08 Toroid.in :default @14:56:07.17 Toroid.seek :clear @14:56:09.72 Carousel.seek 49 @14:56:10.30 Toroid.in :hold @14:56:10.37 Toroid.seek :cartridge @14:56:12.23 Toroid.in :default @14:56:12.32 Toroid.seek :clear @14:56:14.44 Carousel.seek 50 @14:56:14.87 Toroid.in :hold @14:56:14.95 Toroid.seek :cartridge @14:56:16.81 Toroid.in :default @14:56:16.90 Toroid.seek :clear @14:56:19.00 Carousel.seek 51 @14:56:19.42 Toroid.in :hold @14:56:19.50 Toroid.seek :cartridge @14:56:21.36 Toroid.in :default @14:56:21.45 Toroid.seek :clear @14:56:23.55 Carousel.seek 52 @14:56:23.99 Toroid.in :hold @14:56:24.08 Toroid.seek :cartridge @14:56:25.96 Toroid.in :default @14:56:26.05 Toroid.seek :clear @14:56:28.19 Carousel.seek 53 @14:56:28.62 Toroid.in :hold @14:56:28.71 Toroid.seek :cartridge @14:56:30.56 Toroid.in :default @14:56:30.65 Toroid.seek :clear @14:56:32.76 Carousel.seek 54 @14:56:33.19 Toroid.in :hold @14:56:33.28 Toroid.seek :cartridge @14:56:35.14 Toroid.in :default @14:56:35.22 Toroid.seek :clear @14:56:37.32 Carousel.seek 55 @14:56:37.75 Toroid.in :hold @14:56:37.84 Toroid.seek :cartridge @14:56:39.71 Toroid.in :default @14:56:40.29 Toroid.seek :clear @14:56:42.46 Carousel.seek 56 @14:56:42.89 Toroid.in :hold @14:56:42.98 Toroid.seek :cartridge @14:56:44.85 Toroid.in :default @14:56:44.93 Toroid.seek :clear @14:56:47.05 Carousel.seek 57 @14:56:47.48 Toroid.in :hold @14:56:47.57 Toroid.seek :cartridge @14:56:49.43 Toroid.in :default @14:56:49.52 Toroid.seek :clear @14:56:51.63 Carousel.seek 58 @14:56:52.06 Toroid.in :hold @14:56:52.15 Toroid.seek :cartridge @14:56:54.00 Toroid.in :default @14:56:54.09 Toroid.seek :clear @14:56:56.22 Carousel.seek 59 @14:56:56.65 Toroid.in :hold @14:56:56.75 Toroid.seek :cartridge @14:56:58.61 Toroid.in :default @14:56:58.69 Toroid.seek :clear @14:57:00.79 Carousel.seek 60 @14:57:01.22 Toroid.in :hold @14:57:01.31 Toroid.seek :cartridge @14:57:03.17 Toroid.in :default @14:57:03.29 Toroid.seek :clear @14:57:05.13 Sampling 100.0ml @14:57:05.15 SamplePump.setPosition! 0ml @14:57:05.44 Abs.in :default @14:57:05.51 Abs.hold @14:57:05.61 PhaseSeparator.hold @14:57:05.97 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.05psi @14:57:12.57 SamplePump.stop @14:57:12.66 Intake.close @14:57:12.76 Exhaust.close @14:57:13.05 Toroid.in :hold @14:57:13.14 Toroid.seek :bypass @14:57:14.80 Toroid.in :default @14:57:14.90 Toroid.seek :clear @14:57:16.54 Abs.stop @14:57:16.62 PhaseSeparator.stop @14:57:16.87 Scale::Skew::CannotTare in bill -- Intake Head Pressure sensor @31.6% scale out of usable range @14:57:43.81 -> BACsampler.xray @15:08:53.82 -> CleanSampler.source=nil @15:09:39.68 -> cleanToroid @15:09:39.71 Cleans Dry SC positions in slots, begin in 10% Bleach @15:09:40.56 Toroid.seek :clear @15:09:40.76 Carousel.in :default @15:09:40.90 Carousel.home.seek 60 @15:09:41.21 Waiting for bill to answer "Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c" @15:09:43.79 Answered Dry Cartridges have been defined? Fish installed on dry ports? If yes, push return, else control-c"" @15:09:43.91 Priming sample loop w/100ml, bypass w/50ml @15:09:43.95 SamplePump.setPosition! 0ml @15:09:44.03 Bypass.open @15:09:45.58 Intake.open @15:09:47.14 Abs.in :default @15:09:47.26 Abs.hold UserAbort in bill -- User Abort!!