@1785282006.57EDT = =tMAIN =mMAIN_scheduler @EDT1785282007.81 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +149 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +39 "Can"p`,Fyu]I& +352 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +74 #Sea pressure (Abs) tare is 6% of full scale +237 "Can"p`,FyufJ + p`,FyufJ + #tPolling Can every 2:00 +10 #Waiting for espclients... +43 "Can"l`,@yufJ +53 #"tina"Session Begun +1141 #-> slots +10775 "Can"m`x,Hyuh +7976 #"tina"-> slot[60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:1525, lysed2:1525}] +5 !o:SyntaxError: mesg"compile error\ tina:2: syntax error, unexpected tASSOC, expecting $end\ ...0,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {...\ ^:@timestampu: Time aխ:bt[" tina:2:in `topLevelBinding'" slot [60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:1525, lysed2:1525}] +553 "Can"l`J,Lyu| +8439 #"tina"-> Cartridge.aspects +3530 "Can"m`g,Gyu +711 #"tina"-> Cart.aspectsOf :standard, extraDilutions:[1], lysed:1525, lysed2:1525 +1105 #-> Cartridge.aspects +10155 "Can"l`T,Kyu +11971 l`m,Gyu  +8979 #"tina"-> start {fireAll "standard"} +92 #"detached from tina"Cartridge group: standard\ \ +10 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +5 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +20 #Powering core bus +224 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +41 "valve0"DP +3 D +3 "core" ā +6 ."detached from tina"Plunge.configure :default +3 "valve0"D# P ޤ-2 +9 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +12 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 ."detached from tina"Abs.configure :default + "ambient0"J#  +9 ."detached from tina"PhaseSeparator.configure :default +3 "carousel1"G$ +9 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +10 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."detached from tina"PinPort.configure :default +3 "valve1"E$ B22 +6 #"detached from tina"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"D% +3 D& +3 "core" Ă%GtO7 0 +3 "carousel0"F% +9 "syringe0"B& +6 ."Plunge_home"Plunge.home.jog 0 +7 "syringe1"C% +2 "valve0"D) +8 "syringe1"C& +5 "syringe0"B % +5 "valve1"E\ & +24 "core" ă&O7 + Ƅ%M2Q. e + …&~  + Æ%|  + È&= $ ć) + ‰%٩r3  + Ŋ&"O0 +e +8 "valve0"D % +12 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +5 ."PinPort_home"PinPort.home.jog 0 +6 ."Carousel_home"Carousel.home.jog 0 +6 ."Air_home"Air.home.jog 0 +4 "syringe0"B ) +2 "syringe1"C * +3 "syringe0"B* +5 "valve0"D* +3 "valve1"E* +12 "carousel0"F) +3 "syringe1"C) +11 "core" ċ%aGZO8 % + Œ) + Í* + Ž* + Ő* + ď* Ò) + Ƒ) +2 "valve1"E& +7 "syringe0"B% +2 "syringe1"C% +3 "valve0"D& +6 "syringe1"C& +3 "syringe0"B& +3 ."Plunge_home"Plunge.in :homing +3 "valve0"D> (2 +21 ."Plunge_home"Plunge.jog 1470 +10 "valve0"D) +4 "core" œ&O*  + ”%vٱ{i Õ%<?  + Ė&O'  + ×& |W  + ˜&hJp +8 ."PinPort_home"PinPort.in :homing +8 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +5 "valve1"E?2 +3 ."Archive_home"Archive.in :homing +6 "syringe0"B> (2 +6 "syringe1"C> -2 +3 "valve0"D?2 +3 "syringe1"C? (2 +3 "syringe0"B? -2 +2 ."PinPort_home"PinPort.jog 1206 +8 ."Lysis2_home"Lysis2.jog 1616 +10 "valve1"E* +8 "syringe0"B)P +4 ."Lysis1_home"Lysis1.jog 2269 +7 ."Air_home"Air.jog 1243 +6 ."Archive_home"Archive.jog 301 +4 ."Toroid_home"Toroid.jog 1366 +4 "syringe1"C* +9 C) +4 "syringe0"B*- +2 "valve0"D*V +12 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"D ' +25 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +6 "core" ž* +2 ."Archive_home"Archive.setRawPosition! 50 +3 "syringe0"B82 +7 ."Archive_home"Archive.seek :home +3 "syringe0"B!( +14 "core" ›) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +10 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B"' +7 "core" ¡( + Ý) +2 ."Air_home"Air.setRawPosition! 0 +10 ."Archive_home"Archive.in :default +4 "syringe1"C7 +7 "syringe0"B?d PZ<2 +3 ."Air_home"Air.seek :home +6 "syringe1"C#' +9 "core" Ś* + ¢' Ü* + ã' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +5 ."Lysis2_home"Lysis2.in :default +4 ."Air_home"Air.in :default +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 "valve1"E8 N +3 "syringe0"B>d P\\k<2 +6 "syringe1"C>d P]z<2 +2 C82 +3 ."PinPort_home"PinPort.seek :home +11 "valve1"E$( +3 ."Lysis1_home"Lysis1.seek :home +51 "syringe1"C%( +23 "core" å( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +172 "core" ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +7 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +159 "core" Ť( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"E'& +3 "valve0"D(( +6 "core" ŧ&yO( +7 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from tina"PinPort.seek :clear +3 "valve1"E)( +43 "core" ũ( +292 Ĩ( +3 ."detached from tina"Air.seek :clear +3 "syringe1"C*' +8 "core" ê' +2 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C+( +71 "core" ë( +3 ."detached from tina"Lysis2.seek :clear +3 "syringe0"B,'j +77 "core" ¬' +2 ."detached from tina"Archive.seek :clear +4 "syringe0"B-(j +76 "core" ­( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +6 F.% +3 "core" Ʈ%T4WQ  +4 ."detached from tina"Carousel.home.seek 6 +2 "carousel0"F/' +21 "core" Ư' +3 "valve0"D0% +5 "core" İ%^OD !l +4 #"detached from tina"Selecting Cartridge 60 +2 "valve0"D1% +7 "core" ı%^OD !\\ +3 "syringe1"C2& +5 "core" ò&ohl +2 "syringe1"C3% +5 "core" ó%ݯlr +2 "valve1"E4& +4 "core" Ŵ&=OH +3 ."detached from tina"Carousel.seek 60 +3 "carousel0"F5'9 +698 "Can"j`,Iyu $! +427 "core" Ƶ' +4 "carousel0"F6% +3 "core" ƶ%9-'xT 2 +6 ."detached from tina"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +6 "syringe1"C7& +3 "core" ÷&ccU +4 ."detached from tina"Lysis1.in :fast +3 "syringe1"C?d P[<2 +6 ."detached from tina"Lysis1.seek :locked +4 "core" Ƶ' 9M&!Q  +3 "syringe1"C8( +5 !"core"o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 60 + 3 counts (sensed 60 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.028997545409917fU: @currentiR: @type2F:@outPressi!:\ @lostF: @inPressi&:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni9: @pastRLSF: @tagi:: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + Ƶ' +248 ø( +2 "valve0"D9% +8 "core" Ĺ%^O !l +2 ."Plunge"Plunge.seek :locked +3 "valve0"D:'Z +7 "syringe1"C;% +7 "core" û%ݬ]~ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +2 "syringe1"C<'6 +81 "core" ĺ' +24 ü' +3 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F=% +2 "core" ƽ%9[YQ  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" >t +3 "cartridge" t +2 "Cartridge" ?y +5 "cartridge" y%FB(^Jv +3 "Cartridge" @y ' +4 "cartridge" y ܉"MCu "J60008 %l/;CO9 +4 #"detached from tina"Mounted lysis Cartridge board#J60008 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"DA% +4 "core" %ZGOO, +6 "syringe1"CB& +5 "core" &Y +2 "syringe1"CC% +5 "core" %6C [ +2 "carousel0"FD% +3 "core" %9PZ \ +5 "carousel0"FE% +5 "core" %9P \ +117 ."PROCESSING"SPR.command "read_sequences" +35 .SPR.command "stats_on" +9 "carousel0"FF% +3 "core" %9P \ +8 ."PROCESSING"SPR.command "start 0 1" +68 ."SPRlogger"SPR.start /var/log/esp/spr26jul2819h.spr.bz2 +198 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"CG% +5 "core" %6` X +45 "syringe1"CH% +3 "core" %6T T +47 "syringe1"CI% +3 "core" %6: K +47 "syringe1"CJ% +4 "core" %68 Q +46 "syringe1"CK% +4 "core" %67 Q +97 "syringe1"CL% +5 "core" %6Q X +4 "syringe1"CM% +15 "core" %6G U +25 "syringe1"CN% +3 "core" %6] \\ +48 "syringe1"CO% +3 "core" %6f X +47 "syringe1"CP% +3 "core" %6k R +47 "syringe1"CQ% +3 "core" %6\\ T +48 "syringe1"CR% +5 "core" %6_ Y +44 "syringe1"CS% +3 "core" %6^ P +47 "syringe1"CT% +3 "core" %6V U +5 "Can"j`,Iyu.H"x +2 #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -1%) +5 .PinPort.in :locking +5 "valve1"E? B22 +9 ."PROCESSING"PinPort.seek :locked +4 "valve1"EU( N +80 "core" ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +7 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 1525uL +4 "syringe0"B>d P\\k<2 +6 ."posArchive"Archive.seek :empty +4 "syringe1"CV(4 +9 ."posLyS2"Lysis2.seek 1525uL +3 "syringe0"BW( +5 BX'4 +138 "core" ( +165 ' +525 ( +2 ."PROCESSING"delayUntil "20:02:32.01" +1294 #"tina"-> exit +3 #Session Ended +7260 "Can"k`r,Iyu@% +11974 j`%,XyuHv(: +11971 l_,byuA+X +11970 n_,kyuE.v +11970 j_W,pyuI2 +1806 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 BY% +3 "core" %49 +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +4 CZ& +3 "core" &4(46 +49 ."Lysis2mixer"Lysis2.seek 1275uL +5 ."PROCESSING"Lysis1.seek 1275uL +4 "syringe0"B[' +3 "syringe1"C\\( +3485 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +192 #SPR stabilized + "syringe1"C]& +3 "core" & ~ < +4 "syringe0"B^% +5 "core" % ~ +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +312 #Waiting up to 11:00 for SPR to enter state 1 +3513 "Can"l_,oyu7i7 +8250 ."PROCESSING"SPR.command "hold_on" +142 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B_% +3 "core" % * +4 "syringe1"C`& +6 "core" &  7 +50 ."Lysis2mixer"Lysis2.seek 975uL +6 ."PROCESSING"Lysis1.seek 975uL +2 "syringe0"Ba' +4 "syringe1"Cb( +3494 "Can"h_,nys(vw>= +684 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +302 #Waiting up to 1:00:00 for SPR to enter state 7 +9470 "Can"l_3,tyu6Cz +11963 k_-,myu6F +11962 l^,nyu7kJ_* +11965 j^,myu6MH +11962 l^,kyu1aQ?f +11965 k^,_yu;NT +11964 k^,Vyu::XH +11966 n^d,Tyu:,[ +4338 #"SPRlogger"SPRsummary:3.31RIU,243RIU,256RIU,348RIU after 1925s +7628 "Can"m^},Ryu#_ +11967 k^r,Nyu#c +11969 k^',Tyu#h +11969 j],Vyu35k8 +11967 l],[yu#oV +11964 l],Yyu3st +11968 m]q,Xyu4Fw. +6259 "syringe1"Cc& +5 "core" &| +2 "syringe0"Bd% +6 "core" %}e +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +307 #Waiting up to 11:00 for SPR to enter state 1 +3381 "Can"l]W,Xyu3z +8388 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Be% +3 "core" %}|J +55 "syringe1"Cf& +8 "core" &|}; +43 ."Lysis2mixer"Lysis2.seek 825uL +4 ."PROCESSING"Lysis1.seek 825uL +2 "syringe0"Bg' +5 "syringe1"Ch( +2092 "core" ' +4 ( +3 ."PROCESSING"delay 15 seconds +1219 "Can"l]Y,Xyt.~& +284 ."PROCESSING"SPR.command "hold_off" +260 #Waiting up to 1:00:00 for SPR to enter state 7 +11421 "Can"k]d,Tyu3 +11965 l](,Uyu1s\ +11963 m\\,Xyu:s( +11963 k\\,Uyu2vF +11963 l\\s,Syu4zd +11966 k\\,Nyu8 +11968 j\\;,Kyu7 +11967 j\\A,Dyu1\  +11970 l\\,Iyu$p" +11968 n[,Fyu#Z +11968 l\\,Dyu$l +11969 k\\,Dyu1h&6 +11966 j\\,;yu#aT +11967 j],3yu49r +11970 j],6yu1 +4295 #"PROCESSING"SPR analysis completed +59 #"SPRlogger"Ended log spr26jul2819h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +111 "carousel0"Fi% +3 "core" %9 PS \ +8 "sampler0"Hj% +5 "core" % +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +5 "valve1"Ek& +4 "core" & N;O. +3 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"El( +78 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 Fm% +3 "core" %91Pv \ +4 ."detached from tina"Carousel.home.seek 60 +2 "carousel0"Fn'9 +14 "core" ' +2 "carousel0"Fo% +5 "core" %94vT 2 +2 "syringe1"Cp& +5 "core" &pI G +2 ."detached from tina"Plunge.seek :locked +3 "valve0"Dq'Z +4 "core" ' +2 "syringe0"Br& +5 "core" &)  +4 ."Archive"Archive.in :unlocking +3 "syringe1"Cs% +2 "syringe0"B? PZ-2 +6 "core" %6 x +2 ."Archive"Archive.seek :clear +5 "syringe0"Bt(j +6 "syringe1"Cu& +2 ."Air"Air.in :unlocking +5 "syringe1"C> P]z-2 +3 "core" &pA|~ O +3 ."Air"Air.seek :clear +9 "syringe1"Cv' +4 "syringe0"Bw% +5 ."Lysis1"Lysis1.in :unlocking +4 "syringe1"C? P[(2 +3 "core" %~p~ Y +2 ."Lysis1"Lysis1.seek :clear +6 ."Lysis2"Lysis2.in :unlocking +3 "syringe1"Cx( + "syringe0"B> P\\k(2 +8 ."Lysis2"Lysis2.seek :clear +2 "syringe0"By'j +57 "core" ' +233 ( +7 ' +100 ( +3 "sampler0"Hz% +4 "core" %* +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785287454.40EDT @EDT1785287454.98 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +320 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +239 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +80 "valve0"DP +3 D{ +3 "core"  +7 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +4 "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default + "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +3 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default + "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D|% +6 "core" %G;O2 i +2 "valve0"D}& +4 "carousel0"F~% +5 "syringe0"B& +5 "syringe1"C% +20 ."Plunge_home"Plunge.home.jog 0 +5 "syringe1"C& +2 "syringe0"B % +2 "valve1"E& +3 "valve0"D) +20 "core" &}O5  + %LQ= y + &  + Á%݉K + Â&M g + ‰%٢  ň&+O/ ,\\ + Ć) +8 "valve0"D% +8 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 "syringe0"B) +3 "syringe1"C) +4 "syringe0"B\ * +11 "valve1"E * +5 "valve0"D* +4 "carousel0"F) +4 "syringe1"C* +18 "core" ą%TG-GO1  + „) Ã) + Š* + ŋ* + ē* Ô* + Ɨ) +3 "syringe0"B% +8 "syringe1"C% +5 C& +2 "valve1"E& +4 "valve0"D& +12 ."Plunge_home"Plunge.in :homing +5 "syringe0"B & +4 "valve0"D> (2 +8 "core" •%u٥a  + Ø%ݖNP + Ö&OJe + Ň&2O% + Đ&}O+ Œ&mV  +8 ."Plunge_home"Plunge.jog 1480 +7 ."PinPort_home"PinPort.in :homing +4 ."Lysis1_home"Lysis1.in :homing + ."Archive_home"Archive.in :homing +3 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."Lysis2_home"Lysis2.in :homing +2 "valve1"E?2 + "valve0"D ) +2 "syringe1"C? (2 +3 "syringe0"B? -2 +4 "syringe1"C> -2 +2 "valve0"D?2 +2 "syringe0"B> (2 +6 ."PinPort_home"PinPort.jog 1227 +5 "valve1"E* +17 ."Lysis2_home"Lysis2.jog 1618 +5 ."Lysis1_home"Lysis1.jog 1603 +8 ."Archive_home"Archive.jog 1626 + ."Toroid_home"Toroid.jog 1359 +3 ."Air_home"Air.jog 1429 +7 "syringe1"C*C +3 "syringe0"B)R +7 B*Z +2 "valve0"D*O +4 "syringe1"C) +47 "core" č) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D' +23 "core" ĝ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +26 "core" Ï* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 ."Lysis1_home"Lysis1.seek :home +3 "core" Ž) +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe1"C( +3 "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +2 "syringe0"B' +5 "core" Œ* + Û) + ™* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Air_home"Air.setRawPosition! 0 +3 ."Archive_home"Archive.setRawPosition! 50 +2 "valve1"E8 N +2 "syringe1"C7 +7 "syringe0"B82 +4 ."PinPort_home"PinPort.seek :home +4 ."Air_home"Air.seek :home +3 ."Archive_home"Archive.seek :home +4 "syringe1"C'' +3 "valve1"E( +4 "syringe0"B&( +14 "core" Ú( + œ' ç' +3 ."Lysis1_home"Lysis1.in :default +3 ."Air_home"Air.in :default +2 ."Lysis2_home"Lysis2.in :default +2 "syringe1"C?d P[<2 + C>d P]z<2 +2 "syringe0"B>d P\\k<2 +12 "core" ¦( +3 ."Archive_home"Archive.in :default + "syringe0"B?d PZ<2 +222 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D$( +3 "core" Ĥ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +151 "core" ş( +2 ."PinPort_home"PinPort.in :default +7 "valve1"E? B22 +31 ."clearToroid"Toroid.seek :clear +4 "valve0"D.( +2 "valve1"E0& +6 "core" Ű&GQ +42 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E1( +40 "core" ű( +255 Į( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C#' +12 "core" ã' +4 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C2( +67 "core" ò( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B%'j +74 "core" ¥' +3 ."detached from tina"Archive.seek :clear +5 "syringe0"B3(j +74 "core" ³( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 F4% +3 "core" ƴ%9ZXP  +4 ."detached from tina"Carousel.home.seek 60 +2 "carousel0"F"'9 +15 "core" Ƣ' +2 "valve0"D!% +5 "core" ġ%^OC !x +3 #"detached from tina"Selecting Cartridge 54 +2 "valve0"D% +4 "core" đ%^OC !~ +3 "syringe1"C/& +3 "core" ï&]x +3 "syringe1"C6% +5 "core" ö%ݛyp +2 "valve1"E7& +5 "core" ŷ&:OB  +3 ."detached from tina"Carousel.seek 54 +2 "carousel0"F9'3 +145 "core" ƹ' +5 "carousel0"F;% +3 "core" ƻ%316T3 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +3 "syringe1"C+& +3 "core" ë&Uu +4 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C=( +10 "core" ƹ' 3\\6!wQ  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 54 - 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef14.847357879234169): @currentia: @type2F:@outPressiw!:\ @lostF: @inPressi6:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni3: @pastRLSF: @tagi>: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ƹ' +233 ý( +3 "valve0"D % +8 "core" Ġ%^O !r +3 ."Plunge"Plunge.seek :locked +2 "valve0"D*'Z +3 "syringe1"C>% +12 "core" þ%ݚo' +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C?'6 +79 "core" Ī' +23 ÿ' +3 ."detached from tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +4 F@% +5 "core" %3Z9Q"  +7 #"detached from tina"Powering cartridge bus +266 "cartridge_broadcast" ( +4 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +4 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" Ay +3 "cartridge" yF( c +3 "Cartridge" By ' +4 "cartridge" y Ps/J59921 $Ws/C" s/GCO +4 #"detached from tina"Mounted lysis Cartridge board#J59921 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"DC% +3 "core" %ZGFO7  +2 "syringe1"CD& +6 "core" &py +2 "syringe1"CE% +4 "core" %6r K +3 "carousel0"FF% +4 "core" %39uPZ \  +4 "carousel0"FG% +5 "core" %39dPr \  +118 ."PROCESSING"SPR.command "read_sequences" +6 .SPR.command "stats_on" +6 "carousel0"FH% +5 "core" %39wPs \  +6 ."PROCESSING"SPR.command "start 0 1" +69 ."SPRlogger"SPR.start /var/log/esp/spr26jul2821h.spr.bz2 +191 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CI% +3 "core" %6\ no H +48 "syringe1"CJ% +5 "core" %6hm K +45 "syringe1"CK% +3 "core" %6gh W +47 "syringe1"CL% +3 "core" %6ir L +47 "syringe1"CM% +5 "core" %6j J +45 "syringe1"CN% +7 "core" %6 f E +43 "syringe1"CO% +3 "core" %6 k G +47 "syringe1"CP% +3 "core" %6j O +48 "syringe1"CQ% +3 "core" %6g B +48 "syringe1"CR% +5 "core" %6 h I +44 "syringe1"CS% +3 "core" %6a B +47 "syringe1"CT% +3 "core" %6gs M +47 "syringe1"C)% +3 "core" é%6 z G +55 "syringe1"C8% +6 "core" ø%6ce K +4 "Can"j],6yu.v +12 #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -1%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"E-( N +78 "core" ŭ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +4 ."PROCESSING"Lysis1.seek 1525uL +2 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +5 "syringe1"C,(4 +3 ."posLyS2"Lysis2.seek 1525uL +4 "syringe0"BY( +6 BZ'4 +143 "core" ì( +158 ' +530 ( +2 ."PROCESSING"delayUntil "21:21:32.27" +1232 "Can"n\\,7yuB! +11968 m\\,0yuA\\U +11967 k\\,,yuBà +11968 k\\f,0yuDƲ  +11969 l\\,/yu@ & +9156 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 BX% +3 "core" %44} +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +3 CV& +3 "core" &42 1 +47 ."Lysis2mixer"Lysis2.seek 1275uL +6 ."PROCESSING"Lysis1.seek 1275uL +3 "syringe0"B]' +3 "syringe1"C^( +2733 "Can"k^,/ys`| D +754 "core" ' +3 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +139 #SPR stabilized +3 "syringe1"C_& +3 "core" & Ov N +3 "syringe0"B`% +5 "core" % m +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +267 #Waiting up to 11:00 for SPR to enter state 1 +8231 "Can"j^,2yu3R b +3534 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B[% +3 "core" % o  +6 "syringe1"C\\& +4 "core" & r P +48 ."Lysis2mixer"Lysis2.seek 975uL +5 ."PROCESSING"Lysis1.seek 975uL +3 "syringe0"Bc' +3 "syringe1"Cd( +4182 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +301 #Waiting up to 1:00:00 for SPR to enter state 7 +2231 "Can"l^,8yt.ڮ +11965 l^o,1yu4ޮ +11968 k^3,/yu5. +11971 l],.yu7 +11973 k]h,-yu:) +11969 l\\,'yt)\  +11967 k\\,&yu2I\ 4 +11969 m]-, yu6\ R +11544 #"SPRlogger"SPRsummary:4.26RIU,230RIU,245RIU,344RIU after 1924s +424 "Can"k^, yu$sF\ p +11967 m_!,#yu$R\ +11965 l_>,#yu$M\ +11969 l^,"yu1\ +11971 k^T, yu$\ +11969 k], yu$Y  +11969 m]b,yu1 $ +11969 k]6,yu1 B +1488 "syringe1"Ce& +4 "core" &| +4 "syringe0"Bf% +4 "core" %} +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +8156 "Can"j]B,yu1 ` +3612 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Ba% +3 "core" %} +4 "syringe1"Cb& +6 "core" &| +55 ."Lysis2mixer"Lysis2.seek 825uL +5 ."PROCESSING"Lysis1.seek 825uL +4 "syringe0"Bi' +5 "syringe1"Cj( +2091 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +302 #Waiting up to 1:00:00 for SPR to enter state 7 +1203 #"bill"Session Begun +542 #-> cd /varl/log/esp +4 !o:SyntaxError: mesg"6compile error\ bill:1: unknown regexp options - lg:@timestampu: Time :bt[" bill:1:in `topLevelBinding'" ls -ls +4 !o:NoMethodError\ : mesgu:NameError::message)undefined method `-@' for :ls:Symbol:@timestampu: Time 8A: name:-@: args[:bt[" bill:2:in `topLevelBinding'" slots +698 "Can"m^0,yt. ~ +2006 #"bill"-> exit +3 #Session Ended +9959 "Can"n^,yu6 +11967 m_Q,yu5$ +11971 l_6,yu3' +11967 i_+yu2+ +11968 l^,yu6.  +11968 j^C+yu42& 2 +11969 k],yu45 P +11972 l],yu$A9H n +11974 m],yu#= +11968 l^,yu#A +8584 #"bill"Session Begun +308 #-> slot +1227 #-> showlog -100 +1851 "Can"n_n,yu49E +11973 n_,yu$6I +11971 j_b+yu#M  +11971 k_+yu0Q` " +11435 #"PROCESSING"SPR analysis completed +58 #"SPRlogger"Ended log spr26jul2821h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +113 "carousel0"Fk% +4 "core" %3KPc \ +6 "sampler0"Hm% +3 "core" %? +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +51 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +3 "valve1"Eo& +6 "core" & N:}O* ! +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +7 ."detached from tina"PinPort.seek :clear +5 "valve1"Ep( +79 "core" ( +2 ."detached from tina"Carousel.in :default +3 "carousel0"F><` F 22 +6 Fr% +3 "core" %3HTP3 \ +3 ."detached from tina"Carousel.home.seek 54 +3 "carousel0"Fs'3 +17 "core" ' +2 "carousel0"Fu% +4 "core" %3WFQ_  +3 "syringe1"Cw& +4 "core" &pA O +3 ."detached from tina"Plunge.seek :locked +3 "valve0"DU'Z +7 "core" ' +3 "syringe0"B5& +4 "core" µ&)w # +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +5 "syringe1"C:% +7 ."Archive"Archive.seek :clear +2 "syringe0"BW(j +6 "core" ú%6{ e +5 ."Air"Air.in :unlocking +3 "syringe1"C<& +4 C> P]z-2 +7 ."Air"Air.seek :clear +3 "syringe1"Ch' +5 "core" ü&p0 ` +6 ."Lysis1"Lysis1.in :unlocking +4 "syringe1"C? P[(2 +3 "syringe0"Bg% +8 ."Lysis1"Lysis1.seek :clear +3 "syringe1"Cz( +6 "core" %p a +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +8 ."Lysis2"Lysis2.seek :clear +6 "syringe0"B{'j +4 "Can"i^+ysTT @ +35 "core" ' +233 ( +25 ' +80 ( +3 "sampler0"H|% +5 "core" %f +11 ."detached from tina"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +3 .Gate.power :main,:OFF +5 #Safely stopped and ready to power off @1785292193.98EDT @EDT1785292194.62 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +309 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +19 #Powering core bus +232 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +35 "valve0"DP +3 D  +2 "core" ĉ +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from tina"SamplePump.configure :default +3 "sampler0"H#^ #(22 +9 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."detached from tina"Abs.configure :default +3 "ambient0"J#  +8 ."detached from tina"PhaseSeparator.configure :default +3 "carousel1"G$ +8 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +12 ."detached from tina"PinPort.configure :default +3 "valve1"E$ B22 +5 #"detached from tina"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +6 "valve0"D% +6 "core" Ă%G6O1 n +3 "valve0"D& +7 "carousel0"F% +3 ."Plunge_home"Plunge.home.jog 0 +13 "syringe0"B~& +13 "syringe1"C}% +3 C& + "valve0"D) +3 "syringe0"B% +4 "valve1"E& +21 "core" ā&O5 + %MIQ N + &  + %ݍ È&TM ` + ‡%ٯR  + ą) + ŕ&H*N -? +8 "valve0"D% +7 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Air_home"Air.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +7 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +4 "syringe0"B) +5 "syringe1"C ) +3 "syringe0"B* +6 "valve0"Dq* +4 "valve1"Ey* +4 "syringe1"Cv* +3 "carousel0"Ft) +32 "core" Ė%QG"O5 + ˜) + Ì) * + * + * + * + ) +3 "syringe1"Cl& +11 Cx% +6 "syringe0"B(% +5 ."Plunge_home"Plunge.in :homing +8 "valve0"Dn& +2 "valve1"E& +3 "syringe0"B & +10 "valve0"D> (2 +9 ."Plunge_home"Plunge.jog 1482 +4 "valve0"D) +4 "core" &P +2 %ݎK + ¨%sٚ  &O! + ņ&'QO%  + ‹&i  +9 ."Lysis2_home"Lysis2.in :homing +8 ."Lysis1_home"Lysis1.in :homing +5 ."Archive_home"Archive.in :homing +4 ."PinPort_home"PinPort.in :homing +5 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +5 "syringe0"B> (2 +4 "syringe1"C? (2 +5 "syringe0"B? -2 +2 "valve1"E?2 +7 "syringe1"C> -2 +5 "valve0"D?2 +5 ."Lysis2_home"Lysis2.jog 1621 +5 ."Lysis1_home"Lysis1.jog 1602 +6 ."Archive_home"Archive.jog 302 +5 ."PinPort_home"PinPort.jog 1248 +5 ."Air_home"Air.jog 1431 +5 ."Toroid_home"Toroid.jog 1367 +5 "syringe0"B)U +4 "syringe1"C\ *B +4 "syringe0"B*. +2 "valve1"E* +12 "syringe1"C ) +4 "valve0"D*W +18 "core" Ą) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"D' +8 "core" “* +2 ."Archive_home"Archive.setRawPosition! 50 +3 "syringe0"B82 +6 ."Archive_home"Archive.seek :home +3 "syringe0"B( +10 "core" Ď' +2 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +13 "core" ’( +2 ."Archive_home"Archive.in :default + "syringe0"B?d PZ<2 +18 "core" ”) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B' +5 "core" Ê* +6 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +6 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C( +10 "core" Í) + ›' +3 ."Air_home"Air.setRawPosition! 0 +2 ."Lysis2_home"Lysis2.in :default +3 "syringe1"C7 +2 "syringe0"B>d P\\k<2 +11 ."Air_home"Air.seek :home +4 "syringe1"C0' +8 "core" Ù( + Ń* ð' +3 ."PinPort_home"PinPort.setRawPosition! 3150 +8 ."Lysis1_home"Lysis1.in :default +4 "valve1"E8 N +2 ."Air_home"Air.in :default +6 "syringe1"C?d P[<2 +2 C>d P]z<2 +3 ."PinPort_home"PinPort.seek :home +12 "valve1"E( +237 "core" ď* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +48 "valve0"D$( +7 "core" Ĥ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +128 "core" Ş( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +7 "valve0"D4( +2 "core" ş&O& % +8 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +7 ."detached from tina"PinPort.seek :clear +3 "valve1"E!( +44 "core" š( +291 Ĵ( +3 ."detached from tina"Air.seek :clear +3 "syringe1"C' +8 "core" Ñ' +3 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C/( +68 "core" ï( +3 ."detached from tina"Lysis2.seek :clear +3 "syringe0"B''j +76 "core" §' +3 ."detached from tina"Archive.seek :clear +4 "syringe0"B6(j +75 "core" ¶( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +7 F7% +3 "core" Ʒ%3VHQQ  +5 ."detached from tina"Carousel.home.seek 54 +3 "carousel0"F'3 +17 "core" ƚ' +3 "valve0"D% +5 "core" Ĝ%^O0 !o +3 #"detached from tina"Selecting Cartridge 48 +2 "valve0"D&% +8 "core" Ħ%^O/ ! +2 "syringe1"C;& +5 "core" û&Rv +2 "syringe1"C+% +9 "core" ë%ݏx +3 "valve1"E& +4 "core" ŗ&O. ] +3 ."detached from tina"Carousel.seek 48 +3 "carousel0"F"'- +146 "core" Ƣ' +5 "carousel0"F % +4 "core" Ơ%-1ʿiT 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C>& +3 "core" þ&Lx +4 ."detached from tina"Lysis1.in :fast +3 "syringe1"C?d P[<2 +9 ."detached from tina"Lysis1.seek :locked +7 "syringe1"C2( +247 "core" ò( +3 "valve0"D@% +6 "core" %^O\\ !v +3 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +8 "syringe1"C#% +7 "core" ã%ݎ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"CA'6 +81 "core" ĝ' +24 ' +2 ."detached from tina"Carousel.reconfigure :locked +3 "carousel0"F#` F 2\ 2 +7 FB% +3 "core" %-[ʻQ  +7 #"detached from tina"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +7 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" Cy +5 "cartridge" ylClC +4 "Cartridge" Dy  +4 "cartridge" y 8&0J59994 <&0C[^ +5 #"detached from tina"Mounted lysis Cartridge board#J59994 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"DE% +5 "core" %ZG3O ) +4 "syringe1"CF& +5 "core" &l +2 "syringe1"CG% +3 "core" %6 o G +4 "carousel0"FH% +4 "core" %-ʼPN \ +3 "carousel0"FI% +6 "core" %-ʼPs \ +121 ."PROCESSING"SPR.command "read_sequences" +46 .SPR.command "stats_on" +10 "carousel0"FJ% +3 "core" %-ʼPZ \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2822h.spr.bz2 +196 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"CK% +6 "core" %6 f F +44 "syringe1"CL% +3 "core" %6v ^ +47 "syringe1"CM% +3 "core" %6d M +47 "syringe1"CN% +4 "core" %6g R +46 "syringe1"CO% +4 "core" %6; M +46 "syringe1"CP% +3 "core" %6h W +47 "syringe1"CQ% +3 "core" %6 g G +47 "syringe1"CR% +3 "core" %6h N +48 "syringe1"CS% +3 "core" %6k S +52 "syringe1"CT% +4 "core" %6f N +41 "syringe1"C)% +3 "core" é%6l Q +47 "syringe1"C8% +3 "core" ø%6b U +47 "syringe1"C1% +3 "core" ñ%6f W +48 "syringe1"C=% +6 "core" ý%6g L +5 "Can"i^+yu1V M +2 #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -1%) +9 .PinPort.in :locking +3 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +11 "valve1"E3( N +81 "core" ų( +3 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +7 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1525uL +6 "syringe0"B>d P\\k<2 +6 ."posArchive"Archive.seek :empty +5 "syringe1"C%(4 +5 ."posLyS2"Lysis2.seek 1525uL +6 "syringe0"BX( +7 BV'4 +136 "core" å( +162 ' +529 ( +2 ."PROCESSING"delayUntil "22:40:33.84" +5817 "Can"j^4+yuEbY ^ +11967 l]+yuA\\\\Q | +11966 j]+yuA_ +11969 j^,+yuF b +2555 #"bill"Session Ended +9412 "Can"l_0+yuBe +4559 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +3 BZ% +3 "core" %4* +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +5 C,& +2 "core" ì&4$) $ +48 ."Lysis2mixer"Lysis2.seek 1275uL +6 ."PROCESSING"Lysis1.seek 1275uL +3 "syringe0"B_' +5 "syringe1"C`( +3482 "core" ' +5 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +136 #SPR stabilized +2 "syringe1"C[& +3 "core" &  I +3 "syringe0"B\\% +6 "core" %   +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +265 #Waiting up to 11:00 for SPR to enter state 1 +867 "Can"k_+yu6zk +10901 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 + "syringe0"B]% +3 "core" % i  +4 "syringe1"C^& +6 "core" & $ N +48 ."Lysis2mixer"Lysis2.seek 975uL +5 "syringe0"Be' +2 ."PROCESSING"Lysis1.seek 975uL +6 "syringe1"Cf( +852 "Can"k_+ys3so +3323 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +306 #Waiting up to 1:00:00 for SPR to enter state 7 +6831 "Can"k_?+yu3wT0 +11970 l^+yu2 zN +11969 m^G+yu1~Yl +11968 j]+yu6 +11965 k]+yu5W +11967 k]+yu4 +11968 k_@+yu1?y +11970 k_+yu7 +6949 #"SPRlogger"SPRsummary:4.75RIU,260RIU,270RIU,382RIU after 1924s +5020 "Can"k_+yu$C +11967 l_+yu#> +11970 k_+yu$Q7\\ +11970 l^|+yu1 z +11965 j^+yu$   +11969 j]+yu0@# +11969 l^+yu6 +8861 "syringe1"Ca& +3 "core" &| +4 "syringe0"Bb% +4 "core" %} +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +361 #Waiting up to 11:00 for SPR to enter state 1 +733 "Can"l_+yu1* +11076 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Bc% +3 "core" %} +6 "syringe1"Cd& +56 "core" &| +42 ."Lysis2mixer"Lysis2.seek 825uL +6 "syringe0"Bk' +5 ."PROCESSING"Lysis1.seek 825uL +5 "syringe1"Cm( +632 "Can"k`,+ys2s1@ +1455 "core" ' +8 ( +3 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +256 #Waiting up to 1:00:00 for SPR to enter state 7 +8749 "Can"l_+yu2((. +11971 j_=+yu0L +11967 k^+yu2&j +11966 m^6+yu7å +11970 m^+yu3# +11969 l^Q+yu2 +11967 j_`+yu;F +11968 j_+yu3 +11969 k_+yu$&կ +11969 j_W+yu$#< +11971 k^+yu##Z +11972 l^+yu3dx +11970 j^.+yu$B +11970 l^+yu1R +11972 l_+yu1} +6930 #"PROCESSING"SPR analysis completed +53 #"SPRlogger"Ended log spr26jul2822h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +124 "carousel0"Fo% +3 "core" %-ʹP0 \ +6 "sampler0"Hr% +3 "core" %C? +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"Eu& +3 "core" & N;}O- ! +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +3 ."detached from tina"PinPort.seek :clear +2 "valve1"Ew( +78 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 F5% +3 "core" Ƶ%-ʹP{ \ +4 ."detached from tina"Carousel.home.seek 48 +2 "carousel0"F:'- +14 "core" ƺ' +2 "carousel0"F<% +6 "core" Ƽ%-VʹQY  +2 "syringe1"Cg& +5 "core" &p9 W +3 ."detached from tina"Plunge.seek :locked +2 "valve0"D-'Z +3 "core" ĭ' +3 "syringe0"B9& +4 "core" ¹&)k / +5 ."Archive"Archive.in :unlocking +2 "syringe1"C*% +5 "syringe0"B? PZ-2 +3 "core" ê%6 +3 ."Archive"Archive.seek :clear +5 "syringe0"BY(j +4 "syringe1"C?& +3 ."Air"Air.in :unlocking +7 "syringe1"C> P]z-2 +3 "core" ÿ&p8 X +3 ."Air"Air.seek :clear +6 ."Lysis1"Lysis1.in :unlocking +5 "syringe1"Cj' +2 C? P[(2 +2 "syringe0"Bi% +6 ."Lysis1"Lysis1.seek :clear +6 "syringe1"C|( +8 "core" %p D +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +3 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B 'j +57 "core" ' +223 ( +17 ‰' +100 ( +3 "sampler0"H% +4 "core" Ȃ%# +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785296936.17EDT @EDT1785296936.74 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +374 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +29 "valve0"DP +3 D +3 "core" ć +7 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default + "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default + "carousel0"F#<` F 22 +7 ."detached from tina"Abs.configure :default + "ambient0"J#  +11 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default + "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D% +3 D}& +3 "core" Ĉ%G*3N z +3 "carousel0"F~% +8 "syringe0"B& +5 ."Plunge_home"Plunge.home.jog 0 +6 "syringe1"C% +3 C& +4 "syringe0"B% +3 "valve0"D() +3 "valve1"El& +30 "core" &?O+ ] + %MʺQ 6 + &+  + Á%}: + Õ&OK e –%٠K  + &*N , + Ĩ) +10 "valve0"Dx% +7 ."PinPort_home"PinPort.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +3 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +4 "valve1"E * +4 "syringe1"Cn* +4 C) +3 "carousel0"FU) +3 "valve0"D{* +13 "syringe0"Bh) +5 BW* +17 "core" %SG7O(  + ŋ* + * Æ) + ) + * ) + * +3 "syringe0"Bp% +8 "syringe1"Cz& +6 "valve0"D& +3 "valve1"E.& +2 "syringe1"C% +7 "syringe0"Bs& +3 ."Plunge_home"Plunge.in :homing +6 "valve0"D> (2 +10 "core" %rٓ + &IL  + ą&>O + Ů&O  + Ø%$  + &gK +7 ."Plunge_home"Plunge.jog 1478 +7 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Archive_home"Archive.in :homing +2 "valve0"Dv) + "syringe0"B> (2 +2 "syringe1"C> -2 +7 "valve0"D?2 +4 "syringe1"C? (2 +6 "valve1"E?2 +3 "syringe0"B? -2 +7 ."Lysis2_home"Lysis2.jog 1621 +3 ."Air_home"Air.jog 1433 +4 ."Toroid_home"Toroid.jog 1380 +3 ."Lysis1_home"Lysis1.jog 1604 +4 ."PinPort_home"PinPort.jog 1240 +3 "syringe0"B)U +4 ."Archive_home"Archive.jog 1626 +4 "valve0"D *d +3 "syringe1"Cy) +3 Cq*D +2 "valve1"E* +3 "syringe0"B*Z +58 "core" ) +4 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D' +24 "core" Ĕ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +9 "core" ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +5 ."Lysis2_home"Lysis2.seek :home +2 "syringe0"B\ ' +5 "core" ) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +4 ."Air_home"Air.seek :home +3 "syringe1"C ' +5 "core" * + “* + Í' + Š' +5 ."Lysis2_home"Lysis2.in :default +2 ."Lysis1_home"Lysis1.setRawPosition! 50 + ."Archive_home"Archive.setRawPosition! 50 +3 ."Air_home"Air.in :default +2 "syringe0"B>d P\\k<2 + B82 +2 "syringe1"C82 +2 C>d P]z<2 +13 ."Lysis1_home"Lysis1.seek :home +5 ."Archive_home"Archive.seek :home +4 "syringe1"C( +5 "syringe0"B( +63 "core" ń* + Ã( +3 ."Lysis1_home"Lysis1.in :default +3 ."PinPort_home"PinPort.setRawPosition! 3150 + "syringe1"C?d P[<2 +3 "valve1"E8 N +6 ."PinPort_home"PinPort.seek :home +3 "valve1"E( +6 "core" Ÿ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +166 "core" Č* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +3 ."Toroid_home"Toroid.seek :home +2 "valve0"D$( +5 "core" Ĥ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +238 "core" ŏ( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +2 "valve1"E& +5 "core" Ş&O + "valve0"D7( +7 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +41 "core" Ŝ( +299 ķ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C&' +5 "core" æ' +2 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C0( +67 "core" ð( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B;'j +73 "core" »' +4 ."detached from tina"Archive.seek :clear +2 "syringe0"B(j +74 "core" ™( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 F+% +3 "core" ƫ%-Vʹ7P  +4 ."detached from tina"Carousel.home.seek 48 +2 "carousel0"F'- +14 "core" Ɨ' +2 "valve0"D% +5 "core" ě%^O6 !x +3 #"detached from tina"Selecting Cartridge 42 +2 "valve0"D% +5 "core" Ē%^|O1 ! +2 "syringe1"C & +4 "core" à&E2 +2 "syringe1"C>% +5 "core" þ%݊x+ +2 "valve1"Et& +5 "core" &O4  +3 ."detached from tina"Carousel.seek 42 +2 "carousel0"F'( +144 "core" ƚ' +4 "carousel0"F@% +3 "core" %(*=T 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"C#& +3 "core" ã&Cx$ +4 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C/( +247 "core" ï( +3 "valve0"DB% +8 "core" %^tOb ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +4 "syringe1"C% +7 "core" Ñ%ݒ +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CC'6 +85 "core" Ď' +18 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +3 FD% +3 "core" %(W-iP  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +4 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" Ey +3 "cartridge" yFF +3 "Cartridge" Fy  +5 "cartridge" y }ߛ/J59976 / CMh +3 #"detached from tina"Mounted lysis Cartridge board#J59976 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"DG% +3 "core" %ZG O( O +3 "syringe1"CH& +4 "core" &u +3 "syringe1"CI% +3 "core" %6G +3 "carousel0"FJ% +3 "core" %(,PP \ +4 "carousel0"FK% +5 "core" %(-Pq \  +117 ."PROCESSING"SPR.command "read_sequences" +13 .SPR.command "stats_on" +7 "carousel0"FL% +4 "core" %(.Pu \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2823h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste + "valve0"D +105 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CM% +3 "core" %6ɽk* +47 "syringe1"CN% +4 "core" %6ɱt +46 "syringe1"CO% +4 "core" %6ɹs +28 "Can"l`(+yu9{t +19 "syringe1"CP% +3 "core" %6ɾm +47 "syringe1"CQ% +3 "core" %6ɽo +46 "syringe1"CR% +3 "core" %6ɸm +47 "syringe1"CS% +3 "core" %6i +48 "syringe1"CT% +3 "core" %6ɽm +47 "syringe1"C)% +5 "core" é%6ɺi +45 "syringe1"C8% +3 "core" ø%6ɸh +47 "syringe1"C1% +4 "core" ñ%6ɳl +46 "syringe1"C=% +3 "core" ý%6ɷo +49 "syringe1"C!% +4 "core" á%6ɷo +44 "syringe1"C2% +14 "core" ò%6ɼg +36 "syringe1"C6% +3 "core" ö%6k +47 "syringe1"C'% +3 "core" ç%6ɿk +47 "syringe1"CZ% +3 "core" %6ɾh +47 "syringe1"C,% +3 "core" ì%6ɽd +47 "syringe1"CV% +3 "core" %6ɽg +47 "syringe1"C%% +3 "core" å%6ɿf +5 "Can"l`(+yu, + #"PROCESSING"Syringe tare is 50% of full scale at 22C (drifted -1%) +3 .PinPort.in :locking +3 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +2 "valve1"E[( N +78 "core" ( +3 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +3 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 1525uL +6 ."posArchive"Archive.seek :empty +4 "syringe0"B>d P\\k<2 +3 "syringe1"C\\(4 +4 "syringe0"B]( +9 ."posLyS2"Lysis2.seek 1525uL +6 "syringe0"B^'4 +137 "core" ( +161 ' +519 ( +2 ."PROCESSING"delayUntil "23:59:34.59" +10183 "Can"k_+yuB4 +11968 j_c+yuCK, +11969 l_+yuCyJ +11971 l^+yuCh +11836 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +4 B_% +2 "core" %4S +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C`& +2 "core" &4R 7 +53 ."Lysis2mixer"Lysis2.seek 1275uL +4 ."PROCESSING"Lysis1.seek 1275uL +4 "syringe0"Ba' +3 "syringe1"Cb( +54 "Can"j^S+ys[ @EDT1785297610.28 "core" ' +3 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +5 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +137 #SPR stabilized + "syringe1"Cc& +3 "core" & q \\ +4 "syringe0"Bd% +4 "core" %   ( +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +266 #Waiting up to 11:00 for SPR to enter state 1 +5561 "Can"m^b+yu4 +6207 ."PROCESSING"SPR.command "hold_on" +136 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Be% +3 "core" %  # +4 "syringe1"Cf& +6 "core" &  Q +51 ."Lysis2mixer"Lysis2.seek 975uL +8 ."PROCESSING"Lysis1.seek 975uL + "syringe0"Bo' +6 "syringe1"Cr( +4176 "core" ' +8 ( +4 ."PROCESSING"delay 15 seconds +1357 "Can"j_>+yt. +146 ."PROCESSING"SPR.command "hold_off" +297 #Waiting up to 1:00:00 for SPR to enter state 7 +11528 "Can"k_+yu4X +11972 l`+yu7 +11968 k_P+yu8R +11968 l^+yu0: +11969 k^0+yu3!FX +11970 k^ +yu8$v +11974 l^f+yu5(` +11974 k_:+yu2+ +2231 #"SPRlogger"SPRsummary:4.37RIU,243RIU,252RIU,350RIU after 1924s +9741 "Can"k_+yu$</ +11974 k_p+yu$4' +11976 l^+yu$8^ +11970 m^"+yu$;* +11972 l]+yu$@,H +11973 l^ +yu2cDf +11969 l^+yu3G +4115 "syringe1"Cu& +4 "core" &|R +3 "syringe0"B5% +5 "core" µ%}b +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +363 #Waiting up to 11:00 for SPR to enter state 1 +5477 "Can"l_k+yu6J +6331 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +3 "syringe0"B<% +3 "core" ¼%}jD +5 "syringe1"Cg& +3 "core" &|g\\ +60 ."Lysis2mixer"Lysis2.seek 825uL +5 ."PROCESSING"Lysis1.seek 825uL +3 "syringe0"B9' +3 "syringe1"C*( +2094 "core" ¹' +3 ê( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +350 #Waiting up to 1:00:00 for SPR to enter state 7 +1464 "Can"m_q+yt.P +11971 k^}+yu5T +11971 k]+yu8Xv +11975 l]k+yu2[ +11972 k]+yu3_o8 +11971 l^X+yu9wbV +11969 l^+yu7ft +11972 l_'+zu4j +11971 j^+yu1m} +11966 m^;+yu#q +11968 l]+yu$u +11971 k]+yu1<z \ +11972 k^?+yu$}( +11970 k_*+yu$AF +11971 l_+yu4d +11972 m_+yu6, +2265 #"PROCESSING"SPR analysis completed +54 #"SPRlogger"Ended log spr26jul2823h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +122 "carousel0"F?% +3 "core" ƿ%(-nPi \  +6 "sampler0"Hi% +5 "core" %j +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +47 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E3& +3 "core" ų& N;O4  +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E"( +78 "core" Ţ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 F% +3 "core" Ɲ%(,OPY \ +4 ."detached from tina"Carousel.home.seek 42 +2 "carousel0"FX'( +15 "core" ' +2 "carousel0"FA% +5 "core" %(13~T 2 +2 "syringe1"Cm& +5 "core" &p1B _ +3 ."detached from tina"Plunge.seek :locked +2 "valve0"Dk'Z +4 "core" ' +3 "syringe0"B& +3 "core" ‚&)u? % +5 ."Archive"Archive.in :unlocking +3 "syringe1"C% +3 "syringe0"B? PZ-2 +5 "core" Ç%6ɱY +3 ."Archive"Archive.seek :clear +5 "syringe1"Cl& +3 "syringe0"B(j +5 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +2 "syringe1"C' +2 "core" &p+Q e +7 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B% + "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C( +2 "core" •%{p9@ U +7 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B~'j +53 "core" Ö' +227 Á( +16 ' +117 ˆ( +5 "sampler0"H}% +4 "core" %ec +56 ."detached from tina"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +2 .Gate.power :core,:OFF + .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785301678.53EDT @EDT1785301678.72 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +357 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +4 D +2 "core"  +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +6 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +6 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default +4 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default + "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default + "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"Dx% +4 "core" %F,N  +5 "valve0"Dp& +3 ."Plunge_home"Plunge.home.jog 0 +5 "carousel0"Fz% +5 "syringe0"B& +3 "syringe1"Cs% +4 "valve0"D) +5 "syringe1"C.& +7 "syringe0"B-% +5 "valve1"Ew& +26 "core" &O( + %N7Q" P` + ˜&a  %\\ + ą) + î&E o + ­%ِ  &O , +10 ."PinPort_home"PinPort.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +3 "valve0"D|% +2 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +4 "syringe0"B:) +3 "syringe1"C4* +2 "valve1"Ej* +3 "valve0"DY* +2 "syringe0"B * +6 "syringe1"C() +5 "carousel0"Fh) +27 "core" %IGO+  + º) +2 ô* + * +2 * +2 ‰* è) + ) +3 "syringe0"B% +12 "syringe1"C{& +2 "valve0"D & +4 "valve1"EW& +2 "syringe0"Bn& +6 "syringe1"Cv% +8 ."Plunge_home"Plunge.in :homing +5 "valve0"D> (2 +14 ."Plunge_home"Plunge.jog 1489 +2 "valve0"D) +5 "core" †%rٙ]  + &@<  + ċ&O + &O  &iX + %݉Q +5 ."Lysis1_home"Lysis1.in :homing +11 ."Lysis2_home"Lysis2.in :homing +4 ."Archive_home"Archive.in :homing +4 ."PinPort_home"PinPort.in :homing + ."Air_home"Air.in :homing +3 "syringe1"C? (2 +3 ."Toroid_home"Toroid.in :homing +4 "syringe0"B? -2 + B> (2 +3 "syringe1"C> -2 +2 "valve1"E?2 +5 ."Lysis1_home"Lysis1.jog 1604 +11 "valve0"D?2 +4 ."Archive_home"Archive.jog 300 +3 ."Lysis2_home"Lysis2.jog 1621 +5 ."Air_home"Air.jog 1431 +3 ."PinPort_home"PinPort.jog 1239 +4 "syringe1"Cy*D +3 "syringe0"Bq*, +8 ."Toroid_home"Toroid.jog 1366 +4 "syringe0"B)U +2 "syringe1"C) +6 "valve1"E* +3 "valve0"D *V +16 "core" Đ) +3 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +8 ."Plunge_home"Plunge.seek :home +4 "valve0"D$' +4 "core" * +3 ."Archive_home"Archive.setRawPosition! 50 +3 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +3 "syringe0"B( +6 "core" Ĥ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +14 "core" ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +23 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C+( +6 "core" Ä) + “) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Air_home"Air.setRawPosition! 0 + "syringe0"B72 +3 "syringe1"C7 +50 ."Lysis2_home"Lysis2.seek :home +11 ."Air_home"Air.seek :home +3 "syringe1"C' +6 "syringe0"B' +9 "core" ë( + Ş* + Û' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "valve1"E8 N +4 ."Lysis1_home"Lysis1.in :default +4 ."Air_home"Air.in :default +5 ."PinPort_home"PinPort.seek :home +4 "syringe1"C?d P[<2 +3 C>d P]z<2 + "valve1"E ( +12 "core" ’' +2 ."Lysis2_home"Lysis2.in :default + "syringe0"B>d P\\k<2 +204 "core" Č* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +3 "valve0"D>( +3 "core" ľ( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +199 "core" Š( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +2 "valve1"Et& +4 "core" &O 1 +2 "valve0"D ( +7 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +40 "core" Ń( +300 č( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C\ ' +5 "core" Ê' +2 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C@( +67 "core" ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B'j +73 "core" Ÿ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"B#(j +74 "core" £( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 FU% +3 "core" %(Q88Q  +4 ."detached from tina"Carousel.home.seek 42 +2 "carousel0"F'( +14 "core" Ɨ' +3 "valve0"DB% +3 "core" %^O, !j +4 #"detached from tina"Selecting Cartridge 36 + "valve0"D% +5 "core" đ%^O. !| +2 "syringe1"C0& +5 "core" ð&K|L +2 "syringe1"CD% +3 "core" %ݘyE +4 "valve1"E& +4 "core" Ŕ&O. 5 +3 ."detached from tina"Carousel.seek 36 +2 "carousel0"F&'"2 +146 "core" Ʀ' +4 "carousel0"FE% +3 "core" %"2(S 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"CF& +3 "core" &A~L +3 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CG( +3 "core" Ʀ' "6M!Q  +3 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 36 + 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.019179185076093T: @currentiR: @type2F:@outPressi!:\ @lostF: @inPressi:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni6": @pastRLSF: @tagi+: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 Ʀ' +240 ( +2 "valve0"DH% +7 "core" %^OV !z +3 ."Plunge"Plunge.seek :locked +2 "valve0"DI'Z +5 "syringe1"CJ% +11 "core" %ݐv +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CK'6 +79 "core" ' +23 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 FL% +3 "core" %"2TlP \ +7 #"detached from tina"Powering cartridge bus +266 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" My +3 "cartridge" y v8 v8 +4 "Cartridge" Ny  +4 "cartridge" y 5/J59925 Ǜ/ZCi +3 #"detached from tina"Mounted lysis Cartridge board#J59925 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3 "valve0"DO% +3 "core" %ZGO) E +3 "syringe1"CP& +5 "core" &xD  +3 "syringe1"CQ% +4 "core" %6vd > +2 "carousel0"FR% +4 "core" %"2mzPR \ +4 "carousel0"FS% +6 "core" %"2l{Po \ \ +115 ."PROCESSING"SPR.command "read_sequences" +14 .SPR.command "stats_on" +66 "carousel0"FT% +3 "core" %"2mP@ \ +5 ."PROCESSING"SPR.command "start 0 1" +13 ."SPRlogger"SPR.start /var/log/esp/spr26jul2901h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste + "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C)% +4 "core" é%6 m; G +46 "syringe1"C8% +3 "core" ø%6o6 A +47 "syringe1"C1% +3 "core" ñ%6o9 A +47 "syringe1"C=% +3 "core" ý%6 n. E +47 "syringe1"C!% +3 "core" á%6k: ; +47 "syringe1"C2% +4 "core" ò%6jG = +46 "syringe1"C6% +3 "core" ö%6nG C +47 "syringe1"C'% +3 "core" ç%6V > +48 "syringe1"CZ% +3 "core" %6oJ B +47 "syringe1"C,% +3 "core" ì%6nI 9 +47 "syringe1"CV% +3 "core" %6 h\\ E +47 "syringe1"C%% +3 "core" å%6iG > +47 "syringe1"C% +3 "core" Ü%6k5 = +47 "syringe1"C/% +4 "core" ï%6h0 : +8 "Can"m_+yu* + #"PROCESSING"Syringe tare is 50% of full scale at 22C (drifted -1%) +3 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +3 "valve1"E( N +79 "core" ř( +5 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +4 ."PROCESSING"Lysis1.seek 1525uL +2 "syringe0"B>d P\\k<2 +5 ."posArchive"Archive.seek :empty +5 "syringe1"C;(4 +6 ."posLyS2"Lysis2.seek 1525uL +5 "syringe0"B_( +3 B`'4 +139 "core" û( +161 ' +530 ( +2 ."PROCESSING"delayUntil "01:18:36.40" +3216 "Can"l^+yuAh| +11971 k^+yuC +11971 l]+yuF +11975 l^+yuAB +11968 k^+yuB 2 +7173 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +4 B^% +3 "core" %4~) +2 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C\\& +3 "core" &4~; 9 +55 ."Lysis2mixer"Lysis2.seek 1275uL +2 ."PROCESSING"Lysis1.seek 1275uL +5 "syringe0"Bc' +2 "syringe1"Cd( +3486 "core" ' +3 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +189 #SPR stabilized +2 "syringe1"Ce& +3 "core" & ' Q +3 "syringe0"Bf% +5 "core" %   +2 ."PROCESSING"delay 20 seconds +465 "Can"k_:+yu9 6 +1537 ."PROCESSING"SPR.command "start 2 0" +361 #Waiting up to 11:00 for SPR to enter state 1 +10067 "Can"k_^+yu43T +1743 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"Ba% +4 "core" % c5  +3 "syringe1"Cb& +6 "core" & g \\ +49 ."Lysis2mixer"Lysis2.seek 975uL +6 ."PROCESSING"Lysis1.seek 975uL +3 "syringe0"Bu' + "syringe1"C5( +4182 "core" ' +3 õ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +303 #Waiting up to 1:00:00 for SPR to enter state 7 +4021 "Can"l^i+yt.Pr +11964 k]+yu7 +11964 m]J+yu4 +11963 m]o+yu6 +11961 k^?+yu3\ v +11967 k_+yu; +11969 m_+yu4T& +11969 l^+yu5 D +9779 #"SPRlogger"SPRsummary:4.67RIU,255RIU,252RIU,363RIU after 1926s +2188 "Can"j]+yu$QǦb +11964 j\\+yu# +11967 j\\+yu$& +11966 l^+yu11 +11963 k_;+yu$ +11964 m_c+yu# +11964 k_p+yu/0߫ +11714 "syringe1"C<& +3 "core" ü&|hd +4 "syringe0"Bg% +4 "core" %}dy +2 ."PROCESSING"delay 20 seconds +238 "Can"l^+yu8&4 +1765 ."PROCESSING"SPR.command "start 2 0" +361 #Waiting up to 11:00 for SPR to enter state 1 +9838 "Can"i]+yu3{R +1973 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Bo% +3 "core" %}[ +5 "syringe1"Cr& +6 "core" &|k +59 ."Lysis2mixer"Lysis2.seek 825uL +6 ."PROCESSING"Lysis1.seek 825uL +3 "syringe0"B?' +2 "syringe1"Ci( +2094 "core" ¿' +4 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +296 #Waiting up to 1:00:00 for SPR to enter state 7 +5868 "Can"k\\+yu7p +11963 l\\+yu3 +11962 l]+yu1 +11964 i^+yu2 +11964 l^+yu/  +11964 k^+yu3 +11963 k^+yu2)$ +11967 l^+zu5B +11967 j^O+yu# \\` +11966 m]+yu# ~ +11968 m]+yu# +11967 k]`+yu0Y +11967 l]+yu$ +11967 m^1+yu0N +11970 l^Q+yu1!H +9861 #"PROCESSING"SPR analysis completed +59 #"SPRlogger"Ended log spr26jul2901h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +114 "carousel0"F3% +3 "core" Ƴ%"2uP` \  +6 "sampler0"H% +5 "core" ȝ%, +4 ."closeHandoff"Handoff.select :close +3 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"EA& +3 "core" & N:O6  +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"Em( +78 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F% +2 "core" Ƃ%"2kPr \ +4 ."detached from tina"Carousel.home.seek 36 +2 "carousel0"F'"2 +16 "core" Ƈ' +2 "carousel0"Fl% +4 "core" %"22lT" 2\ +3 "syringe1"C& +5 "core" Õ&p.Ҁp b +2 ."detached from tina"Plunge.seek :locked +2 "valve0"D['Z +5 "core" ' +2 "syringe0"B& +5 "core" š&)΀}  +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +2 "syringe1"C% +8 ."Archive"Archive.seek :clear +2 "syringe0"B](j +2 "core" Î%6ʀ{ t +10 ."Air"Air.in :unlocking +3 "syringe1"CC& +2 C> P]z-2 +6 "core" &p0 f ` +4 ."Air"Air.seek :clear +5 ."Lysis1"Lysis1.in :unlocking +2 "syringe0"B*% +2 "syringe1"C9' +4 C? P[(2 +58 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C}( +3 "core" ª%p\\ R +7 ."Lysis2"Lysis2.in :unlocking + "syringe0"B> P\\k(2 +5 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B'j +15 "core" ù' +265 ( +16 ' +45 ( +3 "sampler0"Hx% +4 "core" %9 +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785306420.59EDT @EDT1785306420.78 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +357 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +237 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +29 "valve0"DP +3 Dw +3 "core"  +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +7 ."detached from tina"Archive.configure :default +3 "syringe0"B$d PZ<2 +8 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +3 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D-% +3 D.& +3 "core" ĭ%G!:N  +3 "carousel0"Fs% +8 "syringe0"B& +12 "syringe1"Cz% +5 ."Plunge_home"Plunge.home.jog 0 +16 "syringe1"Cp& +3 "syringe0"B|% +3 "valve1"EW& +3 "valve0"D) +19 "core" Į&O- + %MsQ* j$ + ˜&  + %݆L + &9 { + %ٖLp  &:O$ -M + Ć) +4 ."Lysis1_home"Lysis1.home.jog 0 +12 "valve0"D{% +2 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +3 ."Air_home"Air.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +3 "syringe1"Cv* +3 "syringe0"Bn) +4 "carousel0"F ) +7 "syringe0"Bk* +2 "valve1"E"* +4 "syringe1"C~) +9 "valve0"D* +22 "core" %NGO%  + * + ) + * Ţ* + Ƌ) + ) + ā* +4 "valve1"E& +7 "syringe0"B7% +3 "syringe1"C& +13 "valve0"D& +3 "syringe1"CX% +6 "syringe0"B4& +9 ."Plunge_home"Plunge.in :homing +6 "valve0"D> (2 +6 "core" ň&):O#  + ·%rٙs  Ö&5  + ą&O!  + %݁ + ´&k  +8 ."Plunge_home"Plunge.jog 1483 +6 ."PinPort_home"PinPort.in :homing +4 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 ."Lysis2_home"Lysis2.in :homing +2 ."Toroid_home"Toroid.in :homing +3 ."Air_home"Air.in :homing +4 "valve1"E?2 +2 "valve0"D ) +2 "syringe1"C? (2 +2 "syringe0"B? -2 +4 "valve0"D?2 +6 "syringe1"C> -2 +9 "syringe0"B> (2 +5 ."PinPort_home"PinPort.jog 1250 +9 ."Lysis1_home"Lysis1.jog 276 +2 ."Archive_home"Archive.jog 300 +3 ."Toroid_home"Toroid.jog 1365 +3 ."Air_home"Air.jog 1433 +4 ."Lysis2_home"Lysis2.jog 1621 +3 "valve1"E:* +2 "syringe1"C(* +2 "valve0"Dj*U +2 "syringe0"BY*, +2 "syringe1"C) +2 "syringe0"Bq)U +53 "core" è* +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe1"C82 +2 "core" * +2 ĉ) +3 ."Archive_home"Archive.setRawPosition! 50 +3 ."Plunge_home"Plunge.setRawPosition! 50 +3 ."Lysis1_home"Lysis1.seek :home +2 "syringe0"B82 +3 "valve0"D72 +48 "syringe1"Cy( +5 "Can"l^>+ysV$2 +7 ."Archive_home"Archive.seek :home +14 ."Plunge_home"Plunge.seek :home +4 "syringe0"B( +3 "valve0"D' +9 "core" Ð) + ) + ( +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +4 ."Lysis1_home"Lysis1.in :default +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe0"B72 +2 "syringe1"C?d P[<2 +6 C7 +3 ."Lysis2_home"Lysis2.seek :home +8 ."Air_home"Air.seek :home +3 "syringe0"B' +2 "syringe1"Ct' +14 "core" ź* “( + Ą' + ' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +9 ."Air_home"Air.in :default +3 ."Plunge_home"Plunge.in :default +2 "valve1"E8 N + "syringe1"C>d P]z<2 +4 ."Archive_home"Archive.in :default +6 "valve0"D> P ޤ-2 +2 "syringe0"B?d PZ<2 +3 ."PinPort_home"PinPort.seek :home +8 "valve1"E ( +6 "core" ž' +2 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +183 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +5 ."Toroid_home"Toroid.seek :home +2 "valve0"D>( +3 "core" ľ( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +223 "core" Ō( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +3 "valve1"E & +3 "valve0"DU( +4 "core" Š&O 1 +6 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"EB( +41 "core" ( +299 ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C' +4 "core" Ñ' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C0( +68 "core" ð( +2 ."detached from tina"Lysis2.seek :clear +3 "syringe0"BD'j +73 "core" ' +4 ."detached from tina"Archive.seek :clear +2 "syringe0"B(j +74 "core" ”( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 F% +3 "core" ƛ%"1RwP  +4 ."detached from tina"Carousel.home.seek 36 +2 "carousel0"F+'"2 +15 "core" ƫ' +2 "valve0"D% +4 "core" Ē%^O9 !r +4 #"detached from tina"Selecting Cartridge 30 + "valve0"DE% +5 "core" %^O5 !x +2 "syringe1"C& +5 "core" Ï&C +2 "syringe1"CF% +5 "core" %݋yi +2 "valve1"Eh& +3 "core" &O5 r +4 ."detached from tina"Carousel.seek 30 +2 "carousel0"F'X +145 "core" Ɨ' +4 "carousel0"FH% +5 "core" %X(}S 2 +2 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"CJ& +3 "core" &Esx +3 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C@( +248 "core" ( +4 "valve0"DL% +6 "core" %^OV !} +3 ."Plunge"Plunge.seek :locked +2 "valve0"D$'Z +3 "syringe1"C\ % +9 "core" Ê%ݑ'T +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CM'6 +84 "core" Ĥ' +18 ' +3 ."detached from tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +4 FN% +3 "core" %XV}oQ  +7 #"detached from tina"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" Oy +3 "cartridge" y RI +4 "Cartridge" Py  +4 "cartridge" y k`/ C;R c/J59986 +4 #"detached from tina"Mounted lysis Cartridge board#J59986 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"DQ% +3 "core" %ZGO. H +4 "syringe1"CR& +45 "core" &ym  +2 "syringe1"CS% +5 "core" %6U 8 +2 "carousel0"FT% +5 "core" %X}PT \  +12 "carousel0"F)% +5 "core" Ʃ%X}P7 \  +121 ."PROCESSING"SPR.command "read_sequences" +38 .SPR.command "stats_on" +7 "carousel0"F8% +4 "core" Ƹ%X}Pz \  +6 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26jul2902h.spr.bz2 +198 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste + "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"C1% +4 "core" ñ%6m ? +47 "syringe1"C=% +3 "core" ý%6s : +47 "syringe1"C!% +3 "core" á%6(t * +47 "syringe1"C2% +3 "core" ò%6q 6 +47 "syringe1"C6% +4 "core" ö%6o B +46 "syringe1"C'% +3 "core" ç%6ru > +47 "syringe1"CZ% +3 "core" %6>| 4 +47 "syringe1"C,% +4 "core" ì%6mj > +47 "syringe1"CV% +3 "core" %6&k` , +47 "syringe1"C%% +3 "core" å%6kX 4 +47 "syringe1"C% +3 "core" Ü%6mJ 8 +47 "syringe1"C/% +3 "core" ï%6jN 9 +47 "syringe1"C% +3 "core" Ã%6d\\ C +47 "syringe1"CG% +4 "core" %6iV < +46 "syringe1"C#% +4 "core" ã%6(kY * +47 "syringe1"C% +3 "core" ß%6gh 5 +46 "syringe1"C^% +4 "core" %6ic @ +46 "syringe1"C\\% +3 "core" %6dk A +47 "syringe1"C`% +3 "core" %6hk 3 +5 "Can"l^>+yu'&J<> +2 #"PROCESSING"Syringe tare is 50% of full scale at 22C (drifted -1%) +2 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"E;( N +78 "core" Ż( +5 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +4 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1525uL +5 "syringe0"B>d P\\k<2 +2 ."posArchive"Archive.seek :empty +4 "syringe1"Ce(4 +4 "syringe0"Bf( +7 ."posLyS2"Lysis2.seek 1525uL +10 "syringe0"Ba'4 +133 "core" ( +169 ' +520 ( +3 ."PROCESSING"delayUntil "02:37:39.40" +7314 "Can"l^+yuBY(P +11966 k]+yuA,#n +11968 j]+yuG/Q +11966 k]+yuAA2~ +11966 l]+yuB5 +2850 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 Bb% +3 "core" %4s] +5 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +4 Cc& +3 "core" &4x[ 1 +46 ."Lysis2mixer"Lysis2.seek 1275uL +6 ."PROCESSING"Lysis1.seek 1275uL +3 "syringe0"Bd' +7 "syringe1"C<( +3477 "core" ' +5 ü( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +50 #SPR stabilized + "syringe1"Cg& +3 "core" & Q X +3 "syringe0"Bo% +6 "core" % XS +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +266 #Waiting up to 11:00 for SPR to enter state 1 +2665 "Can"k]+yu6;R +9101 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"Br% +4 "core" % S  +3 "syringe1"Cu& +7 "core" & D M +50 ."Lysis2mixer"Lysis2.seek 975uL +5 ."PROCESSING"Lysis1.seek 975uL +3 "syringe0"B5' +3 "syringe1"C3( +2652 "Can"h]+ysv+A +1529 "core" µ' +6 ó( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +295 #Waiting up to 1:00:00 for SPR to enter state 7 +8634 "Can"l]+yu2G" +11968 k]+yu2J@ +11966 l]+yu/N^ +11967 h]^+yu6Q| +11968 k]+yu1U +11968 j]+yu1X +11965 n]+yu7\\/ +11966 k]+yu:t_ +5156 #"SPRlogger"SPRsummary:4.01RIU,156RIU,141RIU,211RIU after 1923s +6811 "Can"l]+yu$c +11966 l]d+yu$Zg0 +11968 m];+yu#kN +11968 n]\\+yu,ol +11965 l]+yu$s +11962 j]+zu32w +11962 k]+yu7{T +7089 "syringe1"C& +3 "core" Ý&| +4 "syringe0"BA% +4 "core" %} +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +361 #Waiting up to 11:00 for SPR to enter state 1 +2496 "Can"k]+yu3~ +9313 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B% +3 "core" ‚%}!k | +4 "syringe1"Cl& +4 "core" &|j +63 ."Lysis2mixer"Lysis2.seek 825uL +4 ."PROCESSING"Lysis1.seek 825uL +2 "syringe0"B' +3 "syringe1"C( +2094 "core" •' +4 Ú( +3 ."PROCESSING"delay 15 seconds +327 "Can"m]+yt.. +1175 ."PROCESSING"SPR.command "hold_off" +296 #Waiting up to 1:00:00 for SPR to enter state 7 +10495 "Can"o]R+yu0 +11966 l]+yu/)E> +11963 l]u+yu0\\ +11967 m]+yu1@z +11965 l]+yu2 +11967 l]+yu3sb +11968 m]~+yu0 +11968 k]T+yu0S +11967 k]/+yu$.[ +11968 m]+yu#. +11968 k]+yu$L +11968 l]n+yu0}lj +11967 m]+yu# +11970 k]+yu1_ +11971 k]Q+yu1 +5211 #"PROCESSING"SPR analysis completed +59 #"SPRlogger"Ended log spr26jul2902h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +112 "carousel0"F% +3 "core" Ǝ%X}Pr \ +7 "sampler0"HC% +3 "core" % +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +49 ."detached from tina"Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +2 "valve1"E*& +3 "core" Ū& N9O8  +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" ř( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F&% +2 "core" Ʀ%X}|P_ \  +4 ."detached from tina"Carousel.home.seek 30 +2 "carousel0"FI'X +15 "core" ' +2 "carousel0"F_% +4 "core" %X/}S 2 +3 "syringe1"CK& +3 "core" &p2 ^ +4 ."detached from tina"Plunge.seek :locked +2 "valve0"Di'Z +5 "core" ' +2 "syringe0"B?& +3 "core" ¿&)  +6 ."Archive"Archive.in :unlocking +3 "syringe1"Cx% +8 "syringe0"B? PZ-2 +3 "core" %6 _ +3 ."Archive"Archive.seek :clear +5 "syringe1"C-& +6 ."Air"Air.in :unlocking +2 "syringe0"Bw(j + "syringe1"C> P]z-2 +4 "core" í&p8 X +4 ."Air"Air.seek :clear +6 "syringe1"CW' +6 ."Lysis1"Lysis1.in :unlocking +2 "syringe0"B|% +3 "syringe1"C? P[(2 +6 "core" %wpŀ \\ +3 ."Lysis1"Lysis1.seek :clear +6 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +2 "syringe1"Cp( +6 ."Lysis2"Lysis2.seek :clear +2 "syringe0"Bz'j +56 "core" ' +235 ( +6 ' +113 ( +3 "sampler0"H% +55 "core" Ș%[ +12 ."detached from tina"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785311161.47EDT @EDT1785311161.84 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +341 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +8 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +3 Ds +3 "core"  +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +6 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default + "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +3 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default + "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D.% +3 D{& +4 "carousel0"F% +6 "syringe0"B7& +6 "syringe1"C% +4 "core" Į%G N  +6 "syringe1"CX& +13 "syringe0"B4% +5 ."Plunge_home"Plunge.home.jog 0 +8 "valve1"E& +4 "valve0"D[) +14 "core" &O +6 ƈ%M}Q/  + ·&  + Ö%}M + &9 { ´%٘  + Ņ&O$ - + ) +2 ."PinPort_home"PinPort.home.jog 0 +14 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 "valve0"D}% +2 "valve1"E* +3 ."Archive_home"Archive.home.jog 0 +9 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +3 "syringe1"C]* +3 C) +3 "carousel0"Fm) +8 "syringe0"B9* +2 "valve0"D * +2 "syringe0"B) +21 "core" %LGO'  + * + * + Ç) č* + ¹* + †) ) +3 "syringe0"B"% +8 "syringe1"Cv& +6 Ck% +8 "valve1"E& +3 "syringe0"B~& +6 ."Plunge_home"Plunge.in :homing +5 "valve0"Dn& +2 D> (2 +6 "core" ¢%rٝ + &<l  %݃L + Ł&2O  + &k  + &O  +8 ."Plunge_home"Plunge.jog 1486 +9 ."Lysis2_home"Lysis2.in :homing +3 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 "valve0"D() + ."Archive_home"Archive.in :homing +5 "syringe1"C> -2 +3 "syringe0"B> (2 +2 "valve0"D?2 +4 "valve1"E?2 +11 "syringe1"C? (2 +3 "syringe0"B? -2 +6 ."Air_home"Air.jog 1432 +4 ."Lysis2_home"Lysis2.jog 1620 +5 ."PinPort_home"PinPort.jog 1258 +2 ."Lysis1_home"Lysis1.jog 1605 +2 ."Toroid_home"Toroid.jog 1363 +3 ."Archive_home"Archive.jog 1626 +5 "syringe1"CY) +2 "syringe0"B )T +4 "valve1"Eq* +5 "syringe1"C*E +3 "valve0"D:*S +9 "syringe0"B *Z +45 "core" Ĩ) +4 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +3 "valve0"Dj' +23 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +10 "core" ) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +4 ."Air_home"Air.seek :home +2 "syringe1"C>' +5 "core" þ' + ‰) +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 ."Air_home"Air.in :default +3 "syringe0"B72 +2 "syringe1"C>d P]z<2 +6 ."Lysis2_home"Lysis2.seek :home +5 "syringe0"B ' +4 "core" Ð* +  * +8 ."Archive_home"Archive.setRawPosition! 50 +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe1"C82 + "syringe0"B82 +55 ."Lysis1_home"Lysis1.seek :home +13 ."Archive_home"Archive.seek :home +4 "syringe1"C( +3 "syringe0"B( +9 "core" Œ' + * +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Lysis2_home"Lysis2.in :default + "valve1"E8 N +3 "syringe0"B>d P\\k<2 +5 ."PinPort_home"PinPort.seek :home +6 "valve1"EE( +6 "core" Û( + ’( +3 ."Lysis1_home"Lysis1.in :default +2 ."Archive_home"Archive.in :default +2 "syringe1"C?d P[<2 + "syringe0"B?d PZ<2 +180 "core" ĺ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D( +3 "core" ď( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +221 "core" ( +2 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +3 "valve1"EF& + "valve0"Dh( +6 "core" &a O +6 ."detached from tina"PinPort.in :unlocking +4 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"Et( +41 "core" ( +290 ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"Cy' +5 "core" ' +2 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C( +67 "core" Þ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"BH'j +74 "core" ' +2 ."detached from tina"Archive.seek :clear +2 "syringe0"B(j +74 "core" “( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 FJ% +3 "core" %YW}cP  +4 ."detached from tina"Carousel.home.seek 30 +2 "carousel0"F 'X +15 "core" Ƌ' +2 "valve0"D+% +4 "core" ī%^O: ! +3 #"detached from tina"Selecting Cartridge 24 +2 "valve0"DL% +4 "core" %^vO> ! +2 "syringe1"C\ & +5 "core" Ê&Iz +3 "syringe1"C0% +5 "core" ð%ݔ{ +2 "valve1"EN& +4 "core" &?O5  +3 ."detached from tina"Carousel.seek 24 +3 "carousel0"F' +143 "core" Ƅ' +7 "carousel0"F% +5 "core" Ƒ%4dfT\ 2 +3 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"CO& +3 "core" &Fx +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +3 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CP( +248 "core" ( +3 "valve0"DQ% +7 "core" %^O ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"DR'Z +4 "syringe1"CS% +8 "core" %ݐz/ +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CT'6 +84 "core" ' +18 ' +3 ."detached from tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +4 F)% +3 "core" Ʃ%\\dOQ\  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" 8y +3 "cartridge" yI͡BI͡B +4 "Cartridge" 1y  +4 "cartridge" y $/J59995 "/6CG +3 #"detached from tina"Mounted lysis Cartridge board#J59995 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"D=% +3 "core" Ľ%ZGO* C +2 "syringe1"C!& +6 "core" á&s +2 "syringe1"C2% +4 "core" ò%6/t # +2 "carousel0"F6% +5 "core" ƶ%dOPH \ +3 "carousel0"F'% +5 "core" Ƨ%dPPU \ +122 ."PROCESSING"SPR.command "read_sequences" +8 .SPR.command "stats_on" +7 "carousel0"FZ% +61 "core" %dOP@ \ +6 ."PROCESSING"SPR.command "start 0 1" +13 ."SPRlogger"SPR.start /var/log/esp/spr26jul2903h.spr.bz2 +201 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +2 "valve0"D +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C,% +3 "core" ì%6/~ # +48 "syringe1"CV% +3 "core" %64p  +46 "syringe1"C%% +4 "core" å%6-p % +47 "syringe1"C% +3 "core" Ü%6:m  +47 "syringe1"C/% +3 "core" ï%6%q - +47 "syringe1"C% +6 "core" Ã%69j  +44 "syringe1"CG% +3 "core" %69o  +47 "syringe1"C#% +3 "core" ã%6+l ' +47 "syringe1"C% +3 "core" ß%6&l , +47 "syringe1"C^% +3 "core" %6:h  +47 "syringe1"C\\% +3 "core" %6/m # +47 "syringe1"C`% +3 "core" %68ku  +47 "syringe1"CB% +3 "core" %6-ih % +48 "syringe1"C@% +5 "core" %64j_  +44 "syringe1"C% +3 "core" Ô%68ct  +48 "syringe1"CD% +5 "core" %6+d ' +44 "syringe1"Cb% +3 "core" %63c  +47 "syringe1"Cc% +4 "core" %64h  +46 "syringe1"Ca% +3 "core" %68h  +47 "syringe1"Ce% +3 "core" %6.e $ +47 "syringe1"Cg% +3 "core" %6'h + +47 "syringe1"Co% +3 "core" %6"h 0 +47 "syringe1"Cr% +5 "core" %6-f % +45 "syringe1"Cu% +3 "core" %65k|  +47 "syringe1"Cd% +5 "core" %6+hs ' +45 "syringe1"C<% +4 "core" ü%6*jw ( +46 "syringe1"C% +3 "core" Ý%6$e . +5 "Can"k]Q+yu) + #"PROCESSING"Syringe tare is 50% of full scale at 22C (drifted -1%) +3 .PinPort.in :locking +4 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +2 "valve1"EA( N +78 "core" ( +3 ."posArchive"Archive.in :fast +8 ."posLyS2"Lysis2.in :fast +4 "syringe0"B?d PZ<2 +4 ."PROCESSING"Lysis1.seek 1525uL +4 "syringe0"B>d P\\k<2 +5 "syringe1"C(4 +4 ."posArchive"Archive.seek :empty +9 ."posLyS2"Lysis2.seek 1525uL +3 "syringe0"Bl( + B5'4 +137 "core" Â( +163 µ' +146 "Can"i]b+yt|?K +384 "core" ( +2 ."PROCESSING"delayUntil "03:56:39.35" +11582 "Can"l]!+yuA1ģ +11970 l\\+yu@*  +11965 l]<+yuE < +11970 l]`+yu>O4 Z +10149 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 B3% +3 "core" ³%4;\\ +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C& +3 "core" Î&4=n , +49 ."Lysis2mixer"Lysis2.seek 1275uL +2 ."PROCESSING"Lysis1.seek 1275uL +4 "syringe0"BC' +5 "syringe1"C*( +1740 "Can"j]+ysa҉ x +1742 "core" ' +4 ê( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +45 ."PROCESSING"SPR.command "start 2 14" +183 #SPR stabilized + "syringe1"C&& +4 "core" æ& u N +2 "syringe0"B_% +6 "core" % iq \ +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +311 #Waiting up to 11:00 for SPR to enter state 1 +7158 "Can"n]q+yu3* +4607 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"BK% +3 "core" % n +6 "syringe1"C?& +4 "core" ÿ&  N +53 ."Lysis2mixer"Lysis2.seek 975uL +5 ."PROCESSING"Lysis1.seek 975uL +2 "syringe0"Bx' +3 "syringe1"C-( +4181 "core" ' +3 í( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +340 #Waiting up to 1:00:00 for SPR to enter state 7 +1063 "Can"l]U+yt. +11963 k]J+yu0 +11964 i]@+yu4 +11960 k]3+yu2! +11964 k\\+yu2}!, +11963 l\\+yu4!J +11964 k]+yu3!h +11969 k^+yu3! +11964 l_-+yu6! +690 #"SPRlogger"SPRsummary:3.82RIU,42.8RIU,46.4RIU,65.9RIU after 1925s +11274 "Can"m^+yu $! +11966 k]+yu#! +11966 m\\+yu0s(! +11966 k[+yu$% " +11966 j\\L+yu$ ": +11967 j]+yu/"X +11967 l^+yu6P"v +2618 "syringe1"C|& +3 "core" &|u +4 "syringe0"B;% +4 "core" »%}t +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +7026 "Can"m_O+yu1" +4742 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B% +3 "core" —%} +4 "syringe1"C$& +4 "core" ä&| +57 ."Lysis2mixer"Lysis2.seek 825uL +6 ."PROCESSING"Lysis1.seek 825uL +2 "syringe0"Bf' +3 "syringe1"CM( +2094 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +301 #Waiting up to 1:00:00 for SPR to enter state 7 +3104 "Can"j_O+yt. " +11968 l^<+yu7$" +11966 l]/+yu1&(I" +11966 l\\+yu4+# +11966 l[+yu4/F#* +11972 l[+yt(2#H +11969 l\\+yu46h#f +11968 l]+yu49# +11968 k]C+yu$.=s# +11969 l]i+yu$A# +11969 k]c+yu$=E# +11966 l]M+yu50I# +11967 m]'+yu$8M$ +11965 m\\+yu#Q$8 +11967 l\\+yu/OU$V +11965 k\\+yu5Y$t +633 #"PROCESSING"SPR analysis completed +54 #"SPRlogger"Ended log spr26jul2903h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +123 "carousel0"F% +3 "core" ƚ%dOPn \ +6 "sampler0"H% +5 "core" ȕ%3 +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +47 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E& +3 "core" Ř& N:O.  +4 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +3 ."detached from tina"PinPort.seek :clear +3 "valve1"Es( +78 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 F.% +3 "core" Ʈ%dNAPO \ +4 ."detached from tina"Carousel.home.seek 24 +2 "carousel0"F' +16 "core" ƅ' +2 "carousel0"F4% +3 "core" ƴ%3dXS 2 +3 "syringe1"CX& +4 "core" &p< T +4 ."detached from tina"Plunge.seek :locked +2 "valve0"D'Z +4 "core" Ė' +2 "syringe0"B7& +5 "core" ·&)%  +4 ."Archive"Archive.in :unlocking +3 "syringe1"C% +2 "syringe0"B? PZ-2 +6 "core" È%6 V +2 ."Archive"Archive.seek :clear +7 ."Air"Air.in :unlocking +2 "syringe1"C{& + "syringe0"B}(j +2 "syringe1"C> P]z-2 +11 ."Air"Air.seek :clear +2 "syringe1"C"' +5 "core" &p> R +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"Bv% + "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +3 "syringe1"Ck( +3 "core" %pr J +6 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"Bn'j +54 "core" â' +227 ( +54 ' +76 ( +3 "sampler0"H% +3 "core" ȁ%I +11 ."detached from tina"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785315901.85EDT @EDT1785315902.06 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +357 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +8 #Powering core bus +235 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 D~ +3 "core"  +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +3 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +3 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"Di% +4 D& +7 "core" %F>N  + "carousel0"Fp% +5 ."Plunge_home"Plunge.home.jog 0 +11 "syringe0"BW& +4 "syringe1"CI% +5 "valve0"DU) +8 "syringe1"Cw& +4 "syringe0"Bz% +4 "valve1"E[& +25 "core" ę&ZO) B + %LddQ 3 + &  %݀ + &@ t + ) + %ٔQ  &6O& -Q +9 "valve0"D% +7 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Air_home"Air.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +2 ."Lysis2_home"Lysis2.home.jog 0 +2 ."Carousel_home"Carousel.home.jog 0 +3 "valve1"E* +3 "syringe1"C* +5 C]) +7 "syringe0"B * + "valve0"D9* +3 "carousel0"F() +3 "syringe0"BY) +18 "core" ć%IFO&  + ņ* + * + ) * + Ĺ* + ) ƨ) +3 "syringe0"B % +9 "syringe1"C& +5 "syringe0"B & +3 ."Plunge_home"Plunge.in :homing +7 "valve1"EF& + "syringe1"C:% +2 "valve0"Dq& +8 D> (2 +9 ."Plunge_home"Plunge.jog 1493 +4 "valve0"D) +3 "core" ‰%qٕ  + Ð&> +  &hQ + &*@O + ú%݋ + &XO +9 ."Lysis2_home"Lysis2.in :homing +9 ."Lysis1_home"Lysis1.in :homing +3 ."Archive_home"Archive.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +4 "syringe0"B> (2 +2 "syringe1"C? (2 +3 "syringe0"B? -2 +2 "valve1"E?2 +5 "valve0"D?2 +4 "syringe1"C> -2 +5 ."Lysis2_home"Lysis2.jog 1621 +4 ."Lysis1_home"Lysis1.jog 1605 +5 ."Archive_home"Archive.jog 301 +3 ."PinPort_home"PinPort.jog 1249 +3 "syringe0"BE)U +8 ."Toroid_home"Toroid.jog 1355 +6 "syringe1"CJ*E +3 "syringe0"B+*- +6 "valve1"EL* +3 ."Air_home"Air.jog 1431 +10 "valve0"D\ *K +5 "syringe1"C0) +11 "core" ď) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"DN' +18 "core" «* +3 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +3 "core" ' +5 ."Plunge_home"Plunge.in :default + ."Archive_home"Archive.seek :home +3 "valve0"D> P ޤ-2 +2 "syringe0"B>( +25 "core" ¾( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +5 "core" ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +4 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B' +9 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +59 "syringe1"C82 +2 "core" ð) + ›' + * +4 ."PinPort_home"PinPort.setRawPosition! 3150 +6 ."Lysis2_home"Lysis2.in :default + ."Air_home"Air.setRawPosition! 0 +3 ."Lysis1_home"Lysis1.seek :home +2 "valve1"E8 N +3 "syringe1"C7 +5 "syringe0"B>d P\\k<2 +3 "syringe1"C ( +7 ."PinPort_home"PinPort.seek :home +7 ."Air_home"Air.seek :home +4 "valve1"E( +2 "syringe1"C' +11 "core" Ò' + Ì( +3 ."Air_home"Air.in :default +2 ."Lysis1_home"Lysis1.in :default +3 "syringe1"C>d P]z<2 + C?d P[<2 +201 "core" Ċ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +3 ."Toroid_home"Toroid.seek :home +2 "valve0"DO( +5 "core" ( +2 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +194 "core" ő( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +3 "valve1"Em& + "valve0"D ( +6 "core" &O E +11 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"EQ( +40 "core" ( +290 ċ( +2 ."detached from tina"Air.seek :clear +3 "syringe1"CS' +3 "core" ' +4 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C( +67 "core" Þ( +3 ."detached from tina"Lysis2.seek :clear +3 "syringe0"B)'j +73 "core" ©' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"Bj(j +74 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 Fy% +2 "core" %SdgP  +6 ."detached from tina"Carousel.home.seek 24 +4 "carousel0"F8' +15 "core" Ƹ' +2 "valve0"D1% +4 "core" ı%^ O1 !t +4 #"detached from tina"Selecting Cartridge 17 + "valve0"D=% +3 "core" Ľ%^|O) ! +4 "syringe1"C!& +4 "core" á&V~ +3 "syringe1"C2% +4 "core" ò%ݐ +2 "valve1"E6& +5 "core" Ŷ&O. | +3 ."detached from tina"Carousel.seek 17 +2 "carousel0"F'' +165 "core" Ƨ' +5 "carousel0"FZ% +3 "core" %)FS 2 +3 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"C,& +3 "core" ì&cy +4 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CV( +20 "core" Ƨ' UF!P  +3 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 17 + 3 counts (sensed 17 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiP:@pastThresholdF: @voltagef14.837539518900345: @currentiZ: @type2F:@outPressi!:\ @lostF: @inPressiF:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @tagi,: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + Ƨ' +223 ( +3 "valve0"D%% +7 "core" ĥ%^O !x +3 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +5 "syringe1"C/% +7 "core" ï%ݍv +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C'6 +84 "core" Ĝ' +18 Ã' +3 ."detached from tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +4 FG% +3 "core" %ZFsYQ#  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +4 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" #y +2 "cartridge" y' ' +4 "Cartridge" y  +3 "cartridge" y /J59993 /CF +4 #"detached from tina"Mounted lysis Cartridge board#J59993 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D^% +3 "core" %ZGO) Y +2 "syringe1"C\\& +6 "core" &u  +2 "syringe1"C`% +4 "core" %60| " +3 "carousel0"FB% +7 "core" %FsPs \  +4 "carousel0"F@% +6 "core" %FtPC \  +115 ."PROCESSING"SPR.command "read_sequences" +55 .SPR.command "stats_on" +66 "carousel0"F% +3 "core" Ɣ%FsPq \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2905h.spr.bz2 +257 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +2 "valve0"D +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"CD% +3 "core" %66s  +48 "syringe1"Cb% +3 "core" %6/t # +47 "syringe1"Cc% +3 "core" %6$p . +47 "syringe1"Ca% +3 "core" %6,o & +47 "syringe1"Ce% +3 "core" %6"r 0 +47 "syringe1"Cg% +3 "core" %61s ! +47 "syringe1"Co% +3 "core" %6-t % +47 "syringe1"Cr% +3 "core" %6&p , +48 "syringe1"Cu% +3 "core" %6&" , +47 "syringe1"Cd% +3 "core" %6%k - +47 "syringe1"C<% +3 "core" ü%6'k + +47 "syringe1"C% +3 "core" Ý%6&h , +47 "syringe1"Ct% +6 "core" %6%d - +44 "syringe1"CP% +4 "core" %61d ! +47 "syringe1"C% +3 "core" Ó%6(r * +46 "syringe1"CH% +3 "core" %6*g ( +47 "syringe1"C3% +3 "core" ó%64h  +4 "Can"k\\+yu#[r$ + #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -1%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"E( N +78 "core" Ŏ( +3 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +4 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 1525uL +3 ."posArchive"Archive.seek :empty +3 "syringe0"B>d P\\k<2 + "syringe1"C5(4 +3 "syringe0"B( +8 ."posLyS2"Lysis2.seek 1525uL +6 "syringe0"B&'4 +136 "core" õ( +162 ¦' +520 ‚( +6 ."PROCESSING"delayUntil "05:15:41.59" +4612 "Can"l\\C+yu>]e$ +11968 l\\\ +yu=q`$ +11968 k\\+yu?Ac$ +11967 n\\r+yuDf$ +11967 k\\+yu=j#%\ +5699 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 B_% +3 "core" %43 +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +6 CK& +5 "core" &45 6 +55 ."Lysis2mixer"Lysis2.seek 1275uL +6 ."PROCESSING"Lysis1.seek 1275uL +3 "syringe0"B?' + "syringe1"CC( +3487 "core" ( +4 ¿' +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +2 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +135 #SPR stabilized + "syringe1"C*& +3 "core" ê&  M +3 "syringe0"B|% +4 "core" %  +3 ."PROCESSING"delay 20 seconds +1989 "Can"k\\+yu4"o%( +13 ."PROCESSING"SPR.command "start 2 0" +267 #Waiting up to 11:00 for SPR to enter state 1 +11686 "Can"j\\+yt/s8%F +81 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B;% +8 "core" »% w +4 "syringe1"C& +6 "core" ×& \ | G +56 ."Lysis2mixer"Lysis2.seek 975uL +5 ."PROCESSING"Lysis1.seek 975uL +4 "syringe0"B$' +5 "syringe1"Cx( +4179 "core" ¤' +4 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +287 #Waiting up to 1:00:00 for SPR to enter state 7 +5679 "Can"k\\+yu-+{%d +11966 k\\+yu5% +11967 k\\n+yu4% +11969 k\\Z+yu2% +11972 m\\-+yu1% +11970 l\\A+yu/(% +11970 k\\+yu4& +11967 l\\+yu4&6 +8097 #"SPRlogger"SPRsummary:4.46RIU,21.2RIU,25RIU,37.3RIU after 1925s +3871 "Can"l]+yu#&T +11966 l\\+yu$&r +11969 m\\+zu$;K& +11970 l\\+yu11& +11968 k\\+yu$W& +11968 k\\+yu-LE& +11970 l\\+yu2' +10009 "syringe1"C-& +3 "core" í&| +3 "syringe0"B% +6 "core" š%}( u +2 ."PROCESSING"delay 20 seconds +1945 "Can"l\\+yu6M'& +57 ."PROCESSING"SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +11600 "Can"k]1+yt3'D +167 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 + "syringe0"B% +4 "core" •%}!} | +3 "syringe1"C& +6 "core" Ø&| +48 ."Lysis2mixer"Lysis2.seek 825uL +6 ."PROCESSING"Lysis1.seek 825uL +2 "syringe0"B.' +6 "syringe1"C4( +2091 "core" ®' +5 ô( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +308 #Waiting up to 1:00:00 for SPR to enter state 7 +7676 "Can"k\\+yu6K'b +11964 k]+yu/ ' +11967 m]+yu2M' +11968 k]+yu6' +11969 m\\+yu1J' +11970 l\\+yu4o' +11968 k\\l+zu3l( +11970 k\\N+yu0(4 +11971 k\\+yu$$(R +11969 k]+yu$:(p +11969 l^m+yu#6( +11971 k]+yu2V( +11968 n\\c+yu$( +11968 m[~+yu2G( +11970 l[+zu1m) +8010 #"PROCESSING"SPR analysis completed +53 #"SPRlogger"Ended log spr26jul2905h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +125 "carousel0"FX% +3 "core" %FrPh \  +10 "sampler0"H7% +3 "core" ȷ%^\ +5 ."closeHandoff"Handoff.select :close +3 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +2 "valve1"E& +3 "core" ň& N;TO( J +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E{( +78 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 Fv% +3 "core" %FqPI \  +4 ."detached from tina"Carousel.home.seek 17 +2 "carousel0"FA' +15 "core" ' +2 "carousel0"F% +3 "core" Ƅ%5FsT 2 +6 "syringe1"CR& +6 "core" &p7 Y +2 ."detached from tina"Plunge.seek :locked +3 "valve0"Dl'Z +4 "core" ' +2 "syringe0"BT& +4 "core" &)~  +6 ."Archive"Archive.in :unlocking +2 "syringe1"CM% +2 "syringe0"B? PZ-2 +8 ."Archive"Archive.seek :clear +2 "syringe0"Bf(j +3 "core" %6 g +5 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +3 C& +6 ."Air"Air.seek :clear +2 "syringe1"C~' +3 "core" Á&p8 X +6 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"Bi% + "syringe1"C? P[(2 +9 ."Lysis1"Lysis1.seek :clear +49 "syringe1"C[( +5 "core" %p H +7 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"Bz'j +3 "core" ' +275 ( +22 ' +48 ( +3 "sampler0"Hw% +4 "core" %/ +11 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785320643.07EDT @EDT1785320643.63 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +320 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +8 #Powering core bus +235 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +34 "valve0"DP +3 DI +3 "core"  +9 ."detached from tina"Plunge.configure :default +4 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +7 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default + "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +3 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"DW% +3 Dp& +4 "carousel0"F% +8 "core" %G\ O'  + &TO H + "syringe0"B& +6 "syringe1"C % +6 ."Toroid_home"Toroid.home.jog 0 +9 "syringe1"C& +4 "syringe0"B:% +5 "valve1"E & +6 "valve0"Dq* +2 ."Plunge_home"Plunge.home.jog 0 +4 "valve0"DF) +29 "core" ƙ%MFvQ ! + ‡&  + É%݅ + Ð&8 | º%ٟM  + Š&wO! - + * + ) +10 "valve0"D& +9 Ds% +3 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Air_home"Air.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +8 ."Carousel_home"Carousel.home.jog 0 +4 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +4 "syringe0"Bk) + "syringe1"C") +2 "syringe0"B}* +8 "carousel0"Fh) +9 "valve1"En* +3 "syringe1"C* +18 "core" %KGO#  + Ė&_O% + ) â) + * + * + ) Å* +3 "syringe0"BU% +9 "syringe1"CY& +5 "syringe0"B& +9 "valve1"E & +3 ."Toroid_home"Toroid.in :homing +8 ."Plunge_home"Plunge.in :homing +2 "syringe1"C% +5 "valve0"D?2 +2 D> (2 +5 "core" %sٛ  +2 &Ak + &e + ō&%tO Æ%݌ +7 ."Toroid_home"Toroid.jog 1356 +7 ."Plunge_home"Plunge.jog 1487 +3 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +4 ."PinPort_home"PinPort.in :homing + "valve0"D9*L +2 "syringe0"B> (2 +4 "valve0"D]) +3 "syringe1"C> -2 +2 "syringe0"B? -2 +11 "syringe1"C? (2 +2 "valve1"E?2 +2 ."Lysis2_home"Lysis2.jog 1620 +4 ."Air_home"Air.jog 1431 +10 "syringe0"B)T +6 "syringe1"C+) +8 ."Archive_home"Archive.jog 302 +6 ."PinPort_home"PinPort.jog 1244 +4 ."Lysis1_home"Lysis1.jog 1604 +4 "syringe0"BE*. +2 "valve1"EJ* +2 "syringe1"CL*D +53 "core" * +2 ."Archive_home"Archive.setRawPosition! 50 + "syringe0"B82 +4 ."Archive_home"Archive.seek :home +3 "syringe0"B0( +4 "core" ) +3 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"Dm' +55 "core" °( + ë) + ) + ' * +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +14 ."Air_home"Air.setRawPosition! 0 +2 ."Lysis2_home"Lysis2.setRawPosition! 50 + ."Archive_home"Archive.in :default +4 ."Plunge_home"Plunge.in :default +5 "syringe1"C82 + C7 +2 "syringe0"B72 +3 B?d PZ<2 +4 "valve0"D> P ޤ-2 +15 ."Lysis1_home"Lysis1.seek :home +3 ."Lysis2_home"Lysis2.seek :home +4 ."Air_home"Air.seek :home +5 "syringe0"B\ ' +4 "syringe1"CO( +4 C' +15 "core" * + Ñ' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Air_home"Air.in :default +2 "valve1"E8 N + "syringe1"C>d P]z<2 +9 ."PinPort_home"PinPort.seek :home +2 "valve1"Ey( +7 "core" Š' + ( +3 ."Lysis1_home"Lysis1.in :default +2 ."Lysis2_home"Lysis2.in :default +2 "syringe1"C?d P[<2 + "syringe0"B>d P\\k<2 +115 "core" Ĺ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D1( +3 "core" ı( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +284 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +4 "valve1"E!& +2 "valve0"D=( +5 "core" š&O +5 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E2( +40 "core" Ų( +300 Ľ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C' +4 "core" Ò' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C6( +68 "core" ö( +4 ."detached from tina"Lysis2.seek :clear +4 "syringe0"B 'j +74 "core" Œ' +2 ."detached from tina"Archive.seek :clear +2 "syringe0"B(j +74 "core" ›( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +3 F>% +3 "core" ƾ%YFvkP  +4 ."detached from tina"Carousel.home.seek 17 +2 "carousel0"FZ' +15 "core" ' +2 "valve0"D,% +4 "core" Ĭ%^O0 !{ +3 #"detached from tina"Selecting Cartridge 12 +5 "valve0"D(% +6 "core" Ĩ%^vO. ! +2 "syringe1"C8& +4 "core" ø&K} +2 "syringe1"C%% +5 "core" å%ݔ; +2 "valve1"E/& +6 "core" ů&O0 C +2 ."detached from tina"Carousel.seek 12 +3 "carousel0"F'\ +124 "core" ƞ' +4 "carousel0"FG% +4 "core" %\ 61nS 2 +2 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"CN& +3 "core" &Jz +4 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CS( +248 "core" ( +4 "valve0"D#% +7 "core" ģ%^O] !{ +3 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +3 "syringe1"C^% +8 "core" %ݕt` +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C\\'6 +84 "core" ğ' +12 "Can"j[~+zsW)$ +7 "core" ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +3 F`% +3 "core" %\ \\1Q  +8 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" By +3 "cartridge" yՎՎ +4 "Cartridge" @y  +3 "cartridge" y /J59971 Z/MC% +4 #"detached from tina"Mounted lysis Cartridge board#J59971 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3 "valve0"D% +3 "core" Ĕ%ZGO' W +3 "syringe1"CD& +5 "core" &t +2 "syringe1"Cb% +5 "core" %6y +45 "carousel0"Fc% +5 "core" %\ 1Pg \ +13 "carousel0"Fa% +6 "core" %\ 1Pn \ +123 ."PROCESSING"SPR.command "read_sequences" +48 .SPR.command "stats_on" +7 "carousel0"Fe% +4 "core" %\ 1P_ \ +6 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26jul2906h.spr.bz2 +198 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste + "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cg% +3 "core" %6n +47 "syringe1"Co% +4 "core" %6o +47 "syringe1"Cr% +5 "core" %6p +44 "syringe1"Cu% +4 "core" %6p +46 "syringe1"Cd% +5 "core" %6o +45 "syringe1"C<% +3 "core" ü%6p +47 "syringe1"C% +3 "core" Ý%6m +47 "syringe1"Ct% +4 "core" %6o +47 "syringe1"CP% +3 "core" %6k +47 "syringe1"C% +3 "core" Ó%6ɿn +47 "syringe1"CH% +3 "core" %6ɽk +47 "syringe1"C3% +3 "core" ó%6i +47 "syringe1"CQ% +3 "core" %6i +47 "syringe1"CV% +4 "core" %6j +8 "Can"j[~+zu- )) +2 #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -0%) +2 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"Ej( N +80 "core" ( +4 ."posArchive"Archive.in :fast +6 ."posLyS2"Lysis2.in :fast +5 "syringe0"B?d PZ<2 +2 ."PROCESSING"Lysis1.seek 1525uL +4 "syringe0"B>d P\\k<2 +5 "syringe1"C)(4 +6 ."posArchive"Archive.seek :empty +6 ."posLyS2"Lysis2.seek 1525uL +5 "syringe0"BK'4 +2 B_( +135 "core" é( +163 ' +532 ( +3 ."PROCESSING"delayUntil "06:34:41.89" +9397 "Can"l\\k+zu;jH)B +11966 l^0+zu>bu)` +11967 l^+z uC)~ +11263 #"bill"Session Begun +216 #-> slot +492 "Can"m^K+zuA!) +11968 k]+zuB) +1003 ."PROCESSING"Lysis2.in :DAmix +3 "syringe0"B>d2 P\\k<2 +7 B&% +3 "core" ¦%4΀ +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +7 C5& +3 "core" õ&4Z * +58 ."Lysis2mixer"Lysis2.seek 1275uL +5 ."PROCESSING"Lysis1.seek 1275uL +6 "syringe0"B*' +4 "syringe1"C|( +3487 "core" ª' +2 ( +7 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +114 #SPR stabilized +2 "syringe1"C;& +6 "core" û&  P +2 "syringe0"B% +7 "core" —%   +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +261 #Waiting up to 11:00 for SPR to enter state 1 +4420 "Can"k]l+zu1 ) +7347 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B?% +6 "core" ¿% r +3 "syringe1"CC& +6 "core" & t S +49 ."Lysis2mixer"Lysis2.seek 975uL +4 "syringe0"B-' +7 ."PROCESSING"Lysis1.seek 975uL +5 "syringe1"C( +4173 "core" ­' +15 Ú( +2 ."PROCESSING"delay 15 seconds +162 "Can"l\\+zt.) +1340 ."PROCESSING"SPR.command "hold_off" +385 #Waiting up to 1:00:00 for SPR to enter state 7 +10245 "Can"l\\\\+zu6~* +11969 n\\+zu1>*2 +11970 k\\ +zu4y*P +11966 k[+zu7!*n +11967 j[+zu1%n* +11966 l\\a+yu2~)* +11965 k\\+yu5x,* +11969 l\\+z u40* +3487 #"SPRlogger"SPRsummary:5.06RIU,14.7RIU,12.4RIU,17.6RIU after 1925s +8479 "Can"m\\+yu$Q4+ +11994 l\\+yu$(8>+" +11936 l\\+yu$; +11966 n]v+zu$-\\ +11966 l]+z u#-z +11967 m]+zu0- +11966 l\\+zu0e- +2482 #"bill"Session Ended +968 #"PROCESSING"SPR analysis completed +54 #"SPRlogger"Ended log spr26jul2906h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +122 "carousel0"F% +4 "core" ƈ%\ 1Pc \ +6 "sampler0"Hv% +5 "core" %G +4 ."closeHandoff"Handoff.select :close +3 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E& +3 "core" ń& N;O- +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +3 "valve1"ER( +80 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 FT% +3 "core" %\ 1 ^PS \ +4 ."detached from tina"Carousel.home.seek 12 +2 "carousel0"FM'\ +15 "core" ' +2 "carousel0"F% +4 "core" Ɓ%\ 31 S 2 +3 "syringe1"Ci& +4 "core" &p' i +3 ."detached from tina"Plunge.seek :locked +2 "valve0"D'Z +4 "core" Ď' +3 "syringe0"B'& +5 "core" §&)  +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +3 "syringe1"C% +5 ."Archive"Archive.seek :clear +3 "syringe0"B(j +4 "core" Ü%6ɿ`\ +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +2 C& +6 ."Air"Air.seek :clear +3 "syringe1"C4' +4 "core" Ã&p? Q +5 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B.% + "syringe1"C? P[(2 +13 ."Lysis1"Lysis1.seek :clear +2 "syringe1"Cw( +2 "core" ®%p. Q +7 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"BI'j +47 "core" ô' +232 ( +18 ' +95 ‚( +3 "sampler0"Hp% +4 "core" % +11 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785325384.23EDT @EDT1785325384.80 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +320 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +3 DW +2 "core"  +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +67 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +3 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +3 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D % +3 D:& +4 "core" Ġ%FO  +4 "carousel0"F% +7 ."Plunge_home"Plunge.home.jog 0 +7 "syringe0"B & +5 "syringe1"C% +3 C& +4 "valve0"Ds) +6 "syringe0"B% +3 "valve1"E & +26 "core" ĺ&O + Ɛ%M1\ Q Ѝ + ‰&  + Ç%݃ + Ù&3K  –%٨a  + ō&*qN , + ) +8 "valve0"DU% +7 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +4 "syringe0"BY) +3 "syringe1"C) +3 "carousel0"F) +4 "syringe1"C * +5 "syringe0"Bl* +18 "valve0"Dz* +2 "valve1"E~* +18 "core" %IG O$ + ) + Æ) Ë* + ) + * + * * +3 "syringe0"Bf% +7 "syringe1"CA& +5 "syringe0"B{& +3 "valve1"E[& +3 "valve0"Dq& +2 ."Plunge_home"Plunge.in :homing +18 "syringe1"CF% +3 "valve0"D> (2 +14 ."Plunge_home"Plunge.jog 1488 +3 "valve0"Dk) +2 "core" %rٕ  +2 &/J + &fJ + &!O  + &O %݉ +11 ."Lysis2_home"Lysis2.in :homing +5 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."PinPort_home"PinPort.in :homing +4 ."Air_home"Air.in :homing + "syringe0"B> (2 +4 "syringe1"C? (2 +5 "syringe0"B? -2 +3 "valve1"E?2 +2 "valve0"D?2 +3 "syringe1"C> -2 +3 ."Lysis2_home"Lysis2.jog 1621 +6 ."Lysis1_home"Lysis1.jog 277 +8 ."Archive_home"Archive.jog 304 +3 "syringe0"B")U +3 ."PinPort_home"PinPort.jog 1241 +4 ."Air_home"Air.jog 1431 +9 ."Toroid_home"Toroid.jog 1365 +3 "syringe1"C}* +2 "syringe0"Bn*0 +6 "valve1"E* +3 "valve0"D]*U +7 "syringe1"CE) +20 "core" ) +3 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +5 ."Plunge_home"Plunge.seek :home +2 "valve0"DL' +5 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 ."Lysis1_home"Lysis1.seek :home +4 "core" * +2 "syringe1"C( +2 ."Archive_home"Archive.setRawPosition! 50 +6 "syringe0"B82 +5 "core" ' +3 ."Plunge_home"Plunge.in :default +3 ."Archive_home"Archive.seek :home +2 "valve0"D> P ޤ-2 +3 "syringe0"B+( +9 "core" Ï( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +10 "core" ¢) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +2 "core" «( +4 ."Archive_home"Archive.in :default +2 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B?d PZ<2 +3 BJ' +8 "core" ) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +4 ."Air_home"Air.seek :home +2 "syringe1"C9' +5 "core" ù' +3 ."Air_home"Air.in :default +2 "syringe1"C>d P]z<2 +2 "core" ' +4 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +21 "core" Ņ* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +4 ."PinPort_home"PinPort.seek :home +2 "valve1"E!( +269 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D1( +3 "core" ı( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +147 "core" š( +2 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +2 "valve1"Ey& +3 "valve0"D>( +54 "core" &O +5 ."detached from tina"PinPort.in :unlocking +6 "valve1"E? B2 +3 ."detached from tina"PinPort.seek :clear +3 "valve1"E,( +40 "core" Ŭ( +296 ľ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C(' +4 "core" è' +4 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C8( +67 "core" ø( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B%'j +74 "core" ¥' +2 ."detached from tina"Archive.seek :clear +3 "syringe0"B(j +73 "core" ‘( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F/% +2 "core" Ư%\ R1 \\P  +4 ."detached from tina"Carousel.home.seek 12 +2 "carousel0"FO'\ +15 "core" ' +2 "valve0"D\ % +4 "core" Ċ%^O: !y +4 #"detached from tina"Selecting Cartridge 6 + "valve0"DG% +5 "core" %^O4 !{ +2 "syringe1"CN& +5 "core" &Z +2 "syringe1"C0% +5 "core" ð%ݎy +2 "valve1"Eh& +5 "core" &O3 \ +3 ."detached from tina"Carousel.seek 6 +2 "carousel0"FZ' +146 "core" ' +4 "carousel0"F#% +4 "core" ƣ%'6S 2 +2 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"C^& +3 "core" &Z +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +3 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C6( +249 "core" ö( +3 "valve0"D`% +7 "core" %^O ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"Dm'Z +4 "syringe1"C% +8 "core" Ò%ݕq +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CB'6 +84 "core" ' +19 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F@% +3 "core" %S3|Q  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +4 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y +3 "cartridge" yִjִj +3 "Cartridge" Dy  +3 "cartridge" y r/J59943 r/6Cr +4 #"detached from tina"Mounted lysis Cartridge board#J59943 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3 "valve0"Db% +3 "core" %ZG%O% 7 +3 "syringe1"Cc& +6 "core" &t  +2 "syringe1"Ca% +5 "core" %6u +2 "carousel0"Fe% +5 "core" %3P` \ +3 "carousel0"Fg% +6 "core" %4Pu \ +116 ."PROCESSING"SPR.command "read_sequences" +55 .SPR.command "stats_on" +7 "carousel0"Fo% +3 "core" %3Pa \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2907h.spr.bz2 +200 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cr% +4 "core" %6o ~ +47 "syringe1"Cu% +3 "core" %6k } +47 "syringe1"Cd% +3 "core" %6o v +47 "syringe1"C<% +3 "core" ü%6t ~ +47 "syringe1"C% +4 "core" Ý%6l +46 "syringe1"Ct% +3 "core" %6s } +47 "syringe1"CP% +3 "core" %6i z +47 "syringe1"C% +3 "core" Ó%6q +75 "syringe1"CH% +3 "core" %6f { +20 "syringe1"C3% +3 "core" ó%6n +47 "syringe1"CQ% +3 "core" %6c +47 "syringe1"CV% +3 "core" %6h } +47 "syringe1"C2% +3 "core" ò%6o | +47 "syringe1"CS% +3 "core" %6h } +7 "Can"l\\+zu-x-͎ +2 #"PROCESSING"Syringe tare is 50% of full scale at 22C (drifted -0%) +2 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"E( N +79 "core" ś( +5 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +4 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1525uL +5 "syringe0"B>d P\\k<2 +5 "syringe1"C (4 +5 ."posArchive"Archive.seek :empty +5 "syringe0"B&( +6 ."posLyS2"Lysis2.seek 1525uL +9 "syringe0"B5'4 +136 "core" Ì( +164 µ' +520 ¦( +2 ."PROCESSING"delayUntil "07:53:43.05" +2002 "Can"k\\%+z\ u>3- +11968 m[+zuD- +11965 k[+zuD3. +11967 m[A+zuC(D.. +11964 m[P+zu>Mp.L +8411 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +3 BK% +3 "core" %4P +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C)& +3 "core" é&4I 2 +52 ."Lysis2mixer"Lysis2.seek 1275uL +9 ."PROCESSING"Lysis1.seek 1275uL +4 "syringe0"B;' +4 "syringe1"C( +3468 "Can"i\\9+zsc .j +17 "core" »' +3 ×( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +133 #SPR stabilized + "syringe1"C?& +3 "core" ÿ&  K +3 "syringe0"BC% +10 "core" %   +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +262 #Waiting up to 11:00 for SPR to enter state 1 +8973 "Can"j\\+zu1l. +2793 ."PROCESSING"SPR.command "hold_on" +193 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 + "syringe0"B*% +4 "core" ª%   +3 "syringe1"C|& +6 "core" &  U +49 ."Lysis2mixer"Lysis2.seek 975uL +4 "syringe0"B' +3 ."PROCESSING"Lysis1.seek 975uL +5 "syringe1"C( +4178 "core" •' +5 Ø( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +343 #Waiting up to 1:00:00 for SPR to enter state 7 +2871 "Can"l]N+zt.[. +11963 k\\+zu4<. +11963 k\\0+z(u2. +11962 l[+z%u27/ +11964 l[+zu6/ +11965 k[`+zt(D/< +11964 k[3+z*u2/Z +11967 m[+z!u1{D/x +10848 #"SPRlogger"SPRsummary:4.38RIU,9.59RIU,4.22RIU,12.3RIU after 1924s +1122 "Can"m[u+zu$</ +11965 i[+zu#/ +11969 j]+zu$#?/ +11967 k]+z1u.J/ +11962 l]^+z&u $^ 0 +11967 j\\+zu$$G0, +11965 l\\<+zu/0J +11966 l[+zu3l0h +803 "syringe1"C$& +3 "core" ä&| +4 "syringe0"Bx% +3 "core" %} +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +310 #Waiting up to 11:00 for SPR to enter state 1 +8838 "Can"k[+zu300 +2930 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B-% +4 "core" ­%}t +3 "syringe1"C& +7 "core" Ú&|- +58 ."Lysis2mixer"Lysis2.seek 825uL +8 ."PROCESSING"Lysis1.seek 825uL +3 "syringe0"B' +3 "syringe1"Cv( +2095 "core" ˆ' +3 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +293 #Waiting up to 1:00:00 for SPR to enter state 7 +4914 "Can"i[f,zt.W0 +11963 lZ,zu20 +11963 k[),zu1c0 +11964 o\\U,\ z(u30 +11966 l\\, zu6_1 +11967 n],zu31: +11969 k\\,z$u41X +11966 k\\?,zu0~ 1v +11967 k[,zu$ 1 +11967 m[,zu#1 +11962 k[,zu$d!1 +11967 k[6,zu0@1 +11969 k[2,!zu#2 +11969 k\\A,$z/u/k"%2* +11966 n\\,,z,u3%2H +10808 #"PROCESSING"SPR analysis completed +3 !"trapHandler"o:\ fatal: mesg"!Thread(0xb6976a68): deadlock:bt["7/home/esp/esp2/lib/schedule.rb:557:in `mainThread'""/home/esp/esp2/lib/esp.rb:371 @1785330115.39EDT @1785330320.74EDT = =tMAIN =mMAIN_scheduler @EDT1785330322.63 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +27 #>BREAK< ESP3 Gateway v1.44 for core bus +2 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +35 "Can"l\\,$z0u0.2 +348 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +253 "Can"l\\,$z0u Z/2H + l\\,$z0u Z/2H + #tPolling Can every 2:00 +4 #Waiting for espclients... +47 "Can"l\\,)z3u4/2k +79 #"preston"Session Begun +1669 #-> ESP.shutdown +5 .Gate.power :heat,:OFF +2 .Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3677 #-> slots +6524 "Can"m],!zu,0 2k +11970 l\\,z u,02k +11968 m\\Q,z uF1C2k +15 #"preston"-> Cart.unlock +8 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +5 .Gate.power :analytic1,:ON,limit to 4000mA +19 #Powering core bus +265 "valve0"DP +4 D +2 "core" ā +7 ."preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."preston"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."preston"Toroid.configure :default +3 "valve0"D$ ֜2 +9 ."preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."preston"Abs.configure :default + "ambient0"J#  +10 ."preston"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."preston"Archive.configure :default + "syringe0"B$d PZ<2 +9 ."preston"Air.configure :default +3 "syringe1"C#d P]z<2 +6 ."preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +32 ."preston"Lysis2.configure :default + "syringe0"B#d P\\k<2 +9 ."preston"PinPort.configure :default +2 "valve1"E$ B22 +8 #"preston"All elf controllers running firmware version 4.94 +5 .Gate.power :motor,:ON,limit to 5000mA +299 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !"preston"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"I/home/esp/esp2/utils/cartridge/common.rb:490:in `deactivateAndReady'"B/home/esp/esp2/utils/cartridge/carousel.rb:216:in `disengage'"?/home/esp/esp2/utils/cartridge/carousel.rb:224:in `unlock'"#preston:3:in `topLevelBinding'" (2 +4 D?2 +4 ."Plunge_home"Plunge.jog 1482 +4 ."Toroid_home"Toroid.jog 1366 +4 "valve0"D) +2 D*V +9 "core" ”%pE  + Õ&p-A + –&)n ŗ& Q (2 +3 "syringe1"C> -2 +6 "syringe0"B? -2 +2 "syringe1"C? (2 +4 ."PinPort_home"PinPort.jog 3150 +6 ."Lysis2_home"Lysis2.jog 6397 +4 ."Air_home"Air.jog 1247 +6 ."Lysis1_home"Lysis1.jog 6406 +4 "valve1"E* N +5 ."Archive_home"Archive.jog 9650 +4 "syringe0"B) +3 "syringe1"C) +3 C* +2 "syringe0"B*% +20 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"D ' +25 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +46 "core" Ý) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +6 ."Air_home"Air.seek :home +4 "syringe1"C!' +5 "core" á' +2 ."Air_home"Air.in :default +2 "syringe1"C>d P]z<2 +216 "core" œ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"B"' +6 "core" Ě* + Þ* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 ."Toroid_home"Toroid.setRawPosition! 0 +4 "syringe1"C82 +3 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +5 ."Lysis1_home"Lysis1.seek :home +4 "valve0"D#( +3 "syringe1"C$( +12 "core" ¢' + ģ( +3 ."Toroid_home"Toroid.in :default +2 ."Lysis2_home"Lysis2.in :default +5 "valve0"D? ֜2 +2 "syringe0"B>d P\\k<2 +7 "core" ś* + ä( +2 ."PinPort_home"PinPort.setRawPosition! 3150 +4 "valve1"E8 N +4 ."Lysis1_home"Lysis1.in :default +4 "syringe1"C?d P[<2 +5 ."PinPort_home"PinPort.seek :home +3 "valve1"E%( +51 "core" Ÿ* +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +7 ."Archive_home"Archive.seek :home +3 "syringe0"B&( +25 "core" ¦( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +340 "core" ť( +4 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +2 "valve1"E'& +7 "valve0"D(( +55 "core" ŧ& O= +7 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."preston"PinPort.seek :clear +3 "valve1"E)( +43 "core" ũ( +293 Ĩ( +2 ."preston"Air.seek :clear +3 "syringe1"C*' +7 "core" ê' +3 ."preston"Lysis1.seek :clear +2 "syringe1"C+( +68 "core" ë( +4 ."preston"Lysis2.seek :clear +4 "syringe0"B,'j +74 "core" ¬' +3 ."preston"Archive.seek :clear +3 "syringe0"B-(j +77 "core" ­( +2 ."preston"Carousel.in :default + "carousel0"F><` F 22 +6 F.% +4 "core" Ʈ%X4}Q  +3 ."preston"Carousel.home.seek 6 +3 "carousel0"F/' +16 "core" Ư' +3 "valve0"D0% +4 "core" İ%^O4 ! +3 "valve1"E1& +4 "core" ű&O4 p +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +5 F2% +4 "core" Ʋ%M5sQ0  +3 ."preston"Carousel.home.seek 6 +3 "carousel0"F3' +17 "core" Ƴ' +2 "carousel0"F4% +3 "core" ƴ%L5Q  +4 "syringe0"B5& +4 "core" µ&jr +2 "syringe1"C6% +5 "core" ö%ݏ̀ +2 "syringe0"B7% +5 "core" ·%j٥r +2 "syringe1"C8& +5 "core" ø&cr +2 "valve0"D9% +5 "core" Ĺ%^O7 !| +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +7 "valve0"D:'8 +44 "core" ĺ' +3460 #"preston"-> SC.to 22 +5 .Carousel.seek 22 +3 "carousel0"F;' +351 "core" ƻ' +2 "carousel0"F<% +4 "core" Ƽ%4[T 2 +3331 #"preston"-> slot 22=>:lyseHold_phyto +1900 "Can"l\\c,z0u u3-3k +1535 #"preston"-> SC.to 14 +4 .Carousel.seek 14 +6 "carousel0"F=' +185 "core" ƽ' +2 "carousel0"F>% +4 "core" ƾ% 79T 2 +3105 #"preston"-> SC.to 52 +5 .Carousel.seek 52 +3 "carousel0"F?'1 +797 "core" ƿ' +2 "carousel0"F@% +3 "core" %17ۿT< 2 +2435 #"preston"-> to 52 +5 "valve0"DA% +4 "core" %8ZO  +6 "syringe1"CB& +4 "core" &`Ɓ +3 "syringe1"CC% +3 "core" %ݝ +3 "valve1"ED& +4 "core" & O6 S +4 ."preston"Carousel.seek 52 +3 "carousel0"FE'1 +16 "core" ' +4 "carousel0"FF% +3 "core" %14T 2 +4 ."preston"Carousel.reconfigure :align + "carousel0"F#` F 22 +6 "syringe1"CG& +5 "core" &^ +2 ."preston"Lysis1.in :fast +3 "syringe1"C?d P[<2 +6 ."preston"Lysis1.seek :locked +3 "syringe1"CH( +250 "core" ( +3 "valve0"DI% +7 "core" %8ZOj +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +5 "syringe1"CJ% +5 ."Plunge"Plunge.seek :locked +5 "valve0"DK'Z +4 "core" %ݚ +5 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CL'6 +77 "core" ' +27 ' +3 ."preston"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +8 FM% +3 "core" %1\\Q%  +9 "cartridge_broadcast" ( +3 ."preston"Gate.setIR 1750,5000,0,6 +8 "cartridge" + !o:!I2C::DuplicateAddress::Error: mesg"8Multiple cartridge microcontrollers at address 0x09:bt[ "2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +5 ."preston"Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +6 ."preston"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."preston"Gate.power :heat,:ON,limit to 5000mA +13 "Cartridge" Nt +3 "cartridge" t +3 "Cartridge" Oy +4 "cartridge" yִjִj +3 "Cartridge" Py  +3 "cartridge" y r/J59943 r/6Cr +4 #"preston"Mounted lysis Cartridge board#J59943 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +676 #-> slot 52=>:lyseHold_phyto +1529 #-> slot 52=>[:lyseHold_phyto,:dry] +150 #-> to 22 +2 "carousel0"FQ% +6 "core" %1Pc \ +3 "valve0"DR% +4 "core" %ZGO D +4 ."preston"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"ES& +4 "core" &O Q +3 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +6 FT% +4 "core" %1sPd \ +3 ."preston"Carousel.home.seek 52 +3 "carousel0"FU'1 +18 "core" ' +2 "carousel0"FV% +4 "core" %1&S 2 +3 "syringe0"BW& +4 "core" &j  +4 "syringe1"CX% +4 "core" %6? l +5 ."Air"Air.in :unlocking +2 "syringe0"BY% +4 "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +3 "syringe1"CZ' +6 "core" %j٩ +89 ' +2 "syringe1"C[& +4 "core" &ـ +56 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +4 "valve0"D\\% +7 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C]( +6 "core" %ZGO3 > +5 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"D^'8 +97 "core" ' +12 ( +3 ."preston"Carousel.seek 22 +3 "carousel0"F_' +509 "Can"l\\,z-u`430k +127 "core" ' +7 "carousel0"F`% +3 "core" %+[S 2 +3 ."preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"Ca& +4 "core" &B +3 ."preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."preston"Lysis1.seek :locked +3 "syringe1"Cb( +11 "core" ' S[!P  +3 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 22 + 3 counts (sensed 22 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiP:@pastThresholdF: @voltagef14.906268041237114 : @currentiX: @type2F:@outPressi!:\ @lostF: @inPressi[:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @tagid: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + ' +234 ( +2 "valve0"Dc% +8 "core" %8ZOV +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +3 "syringe1"Cd% +3 "valve0"De'Z +8 "core" %ݑ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"Cf'6 +78 "core" ' +28 ' +2 ."preston"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 Fg% +2 "core" %[[YQ  +7 #"preston"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."preston"Gate.setIR 1750,5000,0,6 +15 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +7 ."preston"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."preston"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" hy +4 "cartridge" y]'6(i +3 "Cartridge" iy 5 +6 "cartridge" y ?"UC\\q A"J60003 "dCb Bm/GyCg +3 #"preston"Mounted lysis Cartridge board#J60003 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +576 #-> Cart.unlock +6 .Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"Ej& +4 "core" &O+ M +3 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fk% +3 "core" %[]Pq \ +4 ."preston"Carousel.home.seek 22 +7 "carousel0"Fl' +15 "core" ' +2 "carousel0"Fm% +4 "core" %W[Q?  +3 "syringe0"Bn& +4 "core" &j \ +3 "syringe1"Co% +4 "core" %6 d +4 ."Air"Air.in :unlocking +3 "syringe0"Bp% +4 "syringe1"C> P]z-2 +6 "core" %j٨ +4 ."Air"Air.seek :clear +3 "syringe1"Cq' +95 "core" ' +2 "syringe1"Cr& +4 "core" & ̀ +5 ."Lysis1"Lysis1.in :unlocking +4 "valve0"Ds% +2 "syringe1"C? P[(2 +9 ."Lysis1"Lysis1.seek :clear +2 "syringe1"Ct( +6 "core" %ZGO I +5 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +10 ."Plunge"Plunge.seek :clear +2 "valve0"Du'8 +97 "core" ' +9 ( +10126 "Can"l]`,z1u\ 6B3Nk +11968 k], z5u\ 73lk +3390 #"preston"-> Cart.dry +264 #-> exit +2 #Session Ended @1785337255.14EDT = =tMAIN =mMAIN_scheduler @EDT1785337256.40 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +146 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +224 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +379 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +73 #Sea pressure (Abs) tare is 6% of full scale +246 "Can"rqv+z@to2X + rqv+z@to2X + #tPolling Can every 2:00 +6 #Waiting for espclients... +46 "Can"lq)+z8t2{ +53 #"nadia"Session Begun +692 #-> ESP.ready! +6 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +57 #Powering core bus +264 "valve0"DP +3 D +3 "core" ā +7 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."nadia"SamplePump.configure :default + "sampler0"H#^ #(22 +9 ."nadia"Toroid.configure :default +3 "valve0"D$ ֜2 +9 ."nadia"Carousel.configure :default + "carousel0"F#<` F 22 +9 ."nadia"Abs.configure :default +2 "ambient0"J#  +35 ."nadia"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +7 ."nadia"Lysis1.configure :default + "syringe1"C$d P[<2 +8 ."nadia"Lysis2.configure :default + "syringe0"B#d P\\k<2 +9 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +5 #"nadia"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +263 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !"nadia"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"!nadia:1:in `topLevelBinding'" (2 +5 "core" “%sٞz  + Ô&[ + Ŗ&cO3  + ×%݈{  + •&ؾv%  + Ę&7O1 +7 ."Plunge_home"Plunge.jog 584 +8 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +5 ."Toroid_home"Toroid.in :homing +4 ."PinPort_home"PinPort.in :homing +4 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +4 "valve0"D)H +2 "syringe0"B> (2 +4 "syringe1"C> -2 +2 "valve0"D?2 +5 "valve1"E?2 +2 "syringe1"C? (2 +4 "syringe0"B? -2 +11 ."PinPort_home"PinPort.jog 1307 +7 ."Lysis2_home"Lysis2.jog 1620 +4 ."Lysis1_home"Lysis1.jog 1600 +4 ."Air_home"Air.jog 1431 +4 ."Toroid_home"Toroid.jog 1351 +7 ."Archive_home"Archive.jog 1591 +3 "valve1"E* +3 "syringe0"B)T +4 "syringe1"C) +3 C*@ +2 "valve0"D*G +5 "syringe0"B*7 +22 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"D ' +24 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +48 "core" Ü) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +7 ."Air_home"Air.seek :home +4 "syringe1"C!' +6 "core" ›) + Ý* +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +5 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 "syringe0"B72 +3 "syringe1"C82 +5 ."Lysis2_home"Lysis2.seek :home +4 ."Lysis1_home"Lysis1.seek :home +5 "syringe0"B"' +7 "syringe1"C#( +7 "core" á' + Ÿ* Ś* +3 ."Air_home"Air.in :default +3 ."Archive_home"Archive.setRawPosition! 50 +4 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "syringe1"C>d P]z<2 +2 "syringe0"B82 +2 "valve1"E8 N +15 ."PinPort_home"PinPort.seek :home +6 ."Archive_home"Archive.seek :home +4 "valve1"E$( +3 "syringe0"B%( +11 "core" ¢' + ã( +3 ."Lysis1_home"Lysis1.in :default +2 ."Lysis2_home"Lysis2.in :default +3 "syringe1"C?d P[<2 +2 "syringe0"B>d P\\k<2 +10 "core" ¥( +4 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +192 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +4 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +184 "core" Ť( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +4 "valve0"D'( +5 "valve1"E(& +5 "core" Ũ&R , +3 ."nadia"PinPort.in :unlocking +3 "valve1"E? B2 +6 ."nadia"PinPort.seek :clear +3 "valve1"E)( +44 "core" ũ( +286 ħ( +3 ."nadia"Air.seek :clear +3 "syringe1"C*' +6 "core" ê' +3 ."nadia"Lysis1.seek :clear +3 "syringe1"C+( +69 "core" ë( +3 ."nadia"Lysis2.seek :clear +5 "syringe0"B,'j +73 "core" ¬' +4 ."nadia"Archive.seek :clear +3 "syringe0"B-(j +75 "core" ­( +2 ."nadia"Carousel.in :default + "carousel0"F><` F 22 +7 F.% +3 "core" Ʈ%ATkc  +3 ."nadia"Carousel.home.seek 26 +3 "carousel0"F/'u +37 "core" Ư' +2 "valve0"D0% +5 "core" İ%^OD !h +2 "valve0"D1% +5 "core" ı%^|OC ! +4 "sampler0"H2% +5 "core" Ȳ%D +3 "valve0"D3& +5 "core" ij&MOA e +2 "carousel0"F4% +4 "core" ƴ%uMl  +5 "ambient0"J5% +7 "core" ʵ% +3 "carousel1"G6& +5 "core" Ƕ&N +2 "syringe0"B7& +5 "core" ·&jؙr Q +2 "syringe1"C8% +8 "core" ø%ݾu +5 "syringe1"C9& +7 "core" ù&go +7 "syringe0"B:% +9 "core" º%jٗn +5 "valve1"E;& +9 "core" Ż&OC ^ +1306 #"nadia"-> ESP.reready! +5 ."Plunge_home"Plunge.in :default +7 ."Toroid_home"Toroid.in :default +15 "valve0"D> P ޤ-2 +8 ."Carousel_home"Carousel.in :default +9 ."Archive_home"Archive.in :default +5 "valve0"D? ֜2 +4 ."Air_home"Air.in :default +4 ."Lysis1_home"Lysis1.in :default +8 ."Lysis2_home"Lysis2.in :default +2 ."Plunge_home"Plunge.home.jog 0 +5 "carousel0"F><` F 22 +4 ."PinPort_home"PinPort.in :default +4 "syringe0"B?d PZ<2 +4 "syringe1"C>d P]z<2 +4 ."Toroid_home"Toroid.home.jog 0 +4 "syringe1"C?d P[<2 +4 "syringe0"B>d P\\k<2 +6 "valve0"D<) +2 "valve1"E? B22 +5 ."Carousel_home"Carousel.home.jog 0 +14 "valve0"D=* +4 ."Archive_home"Archive.home.jog 0 +6 ."Air_home"Air.home.jog 0 +7 "core" ļ) + ."Lysis2_home"Lysis2.home.jog 0 +6 ."PinPort_home"PinPort.home.jog 0 +8 "carousel0"F>) +3 ."Lysis1_home"Lysis1.home.jog 0 +6 "syringe0"B?* +4 "core" Ľ* +2 "syringe1"C@) +5 "valve0"DA% +12 "syringe0"BB) +4 "valve1"EC* +3 "syringe1"CD* +3 "valve0"DE& +20 "core" ƾ) + ¿* ) + %^O4 +2 ) + * +2 &NO +2 * +3 "syringe0"BF% +11 "syringe1"CG& + "valve1"EH& +5 "syringe1"CI% +8 ."Toroid_home"Toroid.in :homing +5 "syringe0"BJ& +5 ."Plunge_home"Plunge.in :homing +9 "valve0"D?2 +5 D> (2 +8 ."Toroid_home"Toroid.jog 1355 +2 ."Plunge_home"Plunge.jog 434 +7 "valve0"DK*K + DL) +9 "core" %qٙi + &ik + &O0 + % ݸe + &ؔ1  +7 ."Lysis2_home"Lysis2.in :homing +6 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +5 ."PinPort_home"PinPort.in :homing +3 "syringe0"B> (2 +2 ."Air_home"Air.in :homing +6 "syringe1"C? (2 +3 "syringe0"B? -2 +2 "valve1"E?2 +6 ."Lysis2_home"Lysis2.jog 1621 +7 "syringe1"C> -2 +5 ."PinPort_home"PinPort.jog 1216 +4 ."Archive_home"Archive.jog 1598 +5 ."Lysis1_home"Lysis1.jog 1598 +4 "syringe0"BM)U +4 ."Air_home"Air.jog 1424 +9 "valve1"EN* +2 "syringe0"BO*> +5 "syringe1"CP*> +3 CQ) +14 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +10 ."Plunge_home"Plunge.seek :home +3 "valve0"DR' +25 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +46 "core" ) +4 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +7 ."Lysis2_home"Lysis2.seek :home +4 "core" * + ) +2 "syringe0"BS' +4 ."Air_home"Air.setRawPosition! 0 +6 ."Archive_home"Archive.setRawPosition! 50 +5 "syringe1"C7 +2 "syringe0"B82 +7 ."Archive_home"Archive.seek :home +4 ."Air_home"Air.seek :home +6 "syringe0"BT( +2 "syringe1"CU' +12 "core" * + ' + * + ' +2 ."Lysis2_home"Lysis2.in :default +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 ."PinPort_home"PinPort.setRawPosition! 3150 +4 ."Air_home"Air.in :default +3 "syringe0"B>d P\\k<2 +3 "syringe1"C82 +5 "valve1"E8 N +3 "syringe1"C>d P]z<2 +8 ."Lysis1_home"Lysis1.seek :home +3 ."PinPort_home"PinPort.seek :home +8 "syringe1"CV( +5 "valve1"EW( +10 "core" ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +11 "core" ( +2 ."Lysis1_home"Lysis1.in :default + "syringe1"C?d P[<2 +86 "core" * +40 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"DX( +4 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +264 "core" ( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"EY& +4 "valve0"DZ( +5 "core" &bON < +5 ."nadia"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."nadia"PinPort.seek :clear +3 "valve1"E[( +42 "core" ( +295 ( +3 ."nadia"Air.seek :clear +5 "syringe1"C\\' +4 "core" ' +3 ."nadia"Lysis1.seek :clear +2 "syringe1"C]( +68 "core" ( +3 ."nadia"Lysis2.seek :clear +3 "syringe0"B^'j +75 "core" ' +4 ."nadia"Archive.seek :clear +3 "syringe0"B_(j +78 "core" ( +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F`% +3 "core" %sSlP  +4 ."nadia"Carousel.home.seek 26 +5 "carousel0"Fa'u +16 "core" ' +2 "valve0"Db% +3 "core" %^OM !f +3 "valve0"Dc% +5 "core" %^OD !a +3 "sampler0"Hd% +5 "core" %; +3 "valve0"De& +4 "core" &+OG +3 "carousel0"Ff% +4 "core" %uOl  +3 "ambient0"Jg% +3 "core" %A +3 "carousel1"Gh& +5 "core" &O +2 "syringe0"Bi& +4 "core" &jا C +3 "syringe1"Cj% +7 "core" %ݹ +4 "syringe1"Ck& +3 "core" &_ +3 "syringe0"Bl% +5 "core" %j٧ +2 "valve1"Em& +4 "core" &OI a +374 #"nadia"-> Cart.dry +2515 #-> lysis=:lyseHold_phyto +229 #-> lysys +264 #-> lysis +2095 "Can"kn+z6tp\ YP{ +8154 #"nadia"-> Power.sampler :ON +2 .Gate.power :sampler,:ON +3809 "Can"ilp,/z5s2;pn{ +11967 ik`,^z3s3j a{ +2620 #"nadia"-> 3.times{Sampler.refill};BACsampler.prime +2 .Bypass.open +4 "sampler1"In1 +154 "core" 1 +2 ."nadia"Intake.open +2 "sampler0"Ho1 +142 "core" 1 +3 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 Hp% +2 "core" %( +4 ."nadia"SamplePump.seek 30ml +2 "sampler0"Hq'` +3992 "core" ' + ."nadia"Bypass.close +3 "sampler1"Ir/ +147 "core" / +2 ."nadia"Exhaust.open +3 "sampler0"Hs2 +151 "core" 2 +2 "sampler0"Ht% +4 "core" %`5 +3 ."nadia"SamplePump.seek 55ml +3 "sampler0"Hu'-[ +3336 "core" ' +2 ."nadia"delay 1 second +103 ."closeIntake"Intake.close +4 ."closeExhaust"Exhaust.close +4 "sampler0"Hv/ +4 Hw0 +3 "carousel0"Fx% +8 "core" %uPlbF  +132 / +9 0 +2 ."nadia"Bypass.open + "sampler1"Iy1 +148 "core" 1 +2 ."nadia"Intake.open +2 "sampler0"Hz1 +140 "core" 1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +8 H{% +4 "core" %E +3 ."nadia"SamplePump.seek 30ml +3 "sampler0"H|'` +789 "Can"ij,z8sxK U{ +3202 "core" ' +2 ."nadia"Bypass.close +2 "sampler1"I}/ +151 "core" / +2 ."nadia"Exhaust.open +3 "sampler0"H~2 +144 "core" 2 +2 "sampler0"H% +4 "core" %`Nc +3 ."nadia"SamplePump.seek 55ml +3 "sampler0"H'-[ +3335 "core" ȁ' +2 ."nadia"delay 1 second +103 ."closeIntake"Intake.close +4 "sampler0"H/ +4 ."closeExhaust"Exhaust.close +6 "sampler0"H\ 0 +4 "carousel0"F % +9 "core" Ɖ%uOlFB  +124 Ȃ/ +12 Ȋ0 +2 ."nadia"Bypass.open +2 "sampler1"I1 +154 "core" Ɉ1 +2 ."nadia"Intake.open +2 "sampler0"H1 +142 "core" Ȇ1 +3 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 H% +4 "core" Ȅ%C +3 ."nadia"SamplePump.seek 30ml +3 "sampler0"H'` +3991 "core" ȃ' + ."nadia"Bypass.close +3 "sampler1"I/ +150 "core" Ʌ/ +2 ."nadia"Exhaust.open +3 "sampler0"H 2 +151 "core" ȋ2 +2 "sampler0"H% +3 "core" ȓ%`Q +4 ."nadia"SamplePump.seek 55ml +2 "sampler0"H'-[ +207 "Can"ki,z2sqKN{ +3128 "core" Ȕ' +2 ."nadia"delay 1 second +104 ."closeIntake"Intake.close +7 "sampler0"H/ +4 ."closeExhaust"Exhaust.close +7 "carousel0"F% +6 "sampler0"H0 +2 "core" ƕ%uPlG$  +126 ȗ/ +23 Ș0 +2 "carousel0"F% +4 "core" Ɩ%uOlG  +2 #"nadia"Priming sample loop w/200ml, bypass w/10ml +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +49 ."nadia"Bypass.open +2 "sampler1"I1 +154 "core" ɇ1 +76 "ambient0"J % +4 "core" ʌ%8 +2 ."nadia"Intake.open +3 "sampler0"H1 +154 "core" ȑ1 +2 "ambient0"J % +5 "core" ʍ% +3 #"nadia"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H% +7 "core" ȏ%i3 +43 "sampler0"H% +3 "core" Ȓ%g' +47 "sampler0"H% +3 "core" Ȑ%[B +47 "sampler0"H% +3 "core" ș%X? +47 "sampler0"H% +3 "core" Ȝ%R +47 "sampler0"H% +5 "core" ț%O( +4 "Can"ki,z2s3 + #"nadia"Vacuum tare is 53% of full scale at 24C (drifted 2%) +2 "ambient0"J% +7 "core" ʝ%* +2 "ambient0"J% +5 "core" ʚ%< +2 ."nadia"Abs.in :default +2 "ambient0"J>: e  +5 ."nadia"Abs.hold +2 "ambient0"J+ +5 "core" ʟ+ +3 ."nadia"PhaseSeparator.in :full +2 "carousel1"G? +6 ."nadia"PhaseSeparator.hold +2 "carousel1"G(, +5 "core" Ǩ, +4 "sampler0"H% +4 "core" Ȟ%E5 +3 "sampler0"H&% +5 "core" Ȧ%J+ +5 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }l#(L@3 22Q +7 ."nadia"SamplePump.seek 10ml,1:10 +4 "sampler0"H$'6 +56 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }l#(L@3 22Q +1286 "core" Ȥ' +2 "sampler0"H.% +5 "core" Ȯ%6qt 2 +2 ."nadia"Abs.stop +2 "ambient0"J5 +5 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +6 ."nadia"SamplePump.setPosition! 0ml + "sampler0"H7 +7 ."nadia"Exhaust.open +2 "sampler0"H!2 +144 "core" ȡ2 +73 ."closeBypass"Bypass.close +4 "sampler1"I0/ +154 "core" ɰ/ +73 "sampler0"H1% +3 "core" ȱ%nH +2 "ambient0"J#% +5 "core" ʣ%d- +2 ."nadia"Abs.in :default +2 "ambient0"J>: e  +7 ."nadia"Abs.hold +3 "ambient0"J2+ +5 "core" ʲ+ +3 ."nadia"PhaseSeparator.hold +2 "carousel1"G3, +7 "core" dz, +2 "ambient0"J4% +4 "core" ʴ%1 +3 "sampler0"H5% +5 "core" ȵ%gM +3 "sampler0"H6% +5 "core" ȶ%gO +4 ."nadia"SamplePump.in :start +3 "sampler0"H>^ }l#(L@3 22Q +6 ."nadia"SamplePump.seek 200ml,13:50 +2 "sampler0"H7'G +58 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }l#(L@3 22Q +5780 "Can"iif,z2spK$\\{ +11964 ji,z2stK;-3{ +8467 "core" ȷ' +2 "sampler0"H8% +5 "core" ȸ%G= 2 +2 ."nadia"Abs.stop +2 "ambient0"J5 +5 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +3482 "Can"kh,z>s2b5%"{ +6849 #"nadia"-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +1718 #-> lysis +3397 "Can"mh,z6tv\ v9@{ +5772 #"nadia"-> SC +7 "carousel0"F9% +3 "core" ƹ%uPl  +629 #"nadia"-> SC.to 60 +5 .Carousel.seek 60 +3 "carousel0"F"'9 +717 "core" Ƣ' +2 "carousel0"F:% +4 "core" ƺ%9=& 2 +316 #"nadia"-> SC.to 1 +4 .Carousel.seek 1 +4 "carousel0"F;' +1228 "core" ƻ' +2 "carousel0"F%% +5 "core" ƥ%> 2 +3263 "Can"lh,z1tr\ :^{ +2598 #"nadia"-> Cartridge.lock +6 "carousel0"F% +4 "core" Ǝ%Z  +5 ."nadia"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +5 "syringe1"CE& +4 "core" &i +4 ."nadia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."nadia"Lysis1.seek :locked +3 "syringe1"CA( +250 "core" ( +2 "valve0"DF% +7 "core" %^O> !Y +3 ."Plunge"Plunge.seek :locked +3 "valve0"DG'Z +5 "syringe1"CI% +10 "core" %ݷ9 +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CJ'6 +78 "core" ' +26 ' +2 ."nadia"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 FH% +3 "core" %]Cu  +6 #"nadia"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."nadia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +8 ."nadia"Gate.power :cartridge,:OFF +4 #Mounted archive Cartridge +4871 #-> Cart.unlock +5 "valve1"E & +3 "core" Š&OX g +3 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F'% +2 "core" Ƨ% \  +4 ."nadia"Carousel.home.seek 1 +2 "carousel0"F/' +48 "core" Ư' +2 "carousel0"F-% +4 "core" ƭ%O  +3 "syringe0"B*& +4 "core" ª&jؑ Y +3 "syringe1"C,% +4 "core" ì%6o D +13 ."Air"Air.in :unlocking +4 "syringe1"C> P]z-2 +4 "syringe0"B)% +8 ."Air"Air.seek :clear +2 "syringe1"C+' +4 "core" ©%jٙ +91 ë' +2 "syringe1"C=& +4 "core" ý&€ +5 ."Lysis1"Lysis1.in :unlocking +2 "valve0"D<% +4 "syringe1"C? P[(2 +8 "core" ļ%ZGOX +4 ."Lysis1"Lysis1.seek :clear +5 "syringe1"CB( +4 ."Plunge"Plunge.in :unlocking +4 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +3 "valve0"DC'8 +96 "core" ' +19 ( +364 #"nadia"-> Cart.lock +6 "carousel0"F?% +3 "core" ƿ%O  +3 ."nadia"Carousel.reconfigure :align + "carousel0"F#` F 22 +5 "syringe1"CD& +4 "core" &Y +3 ."nadia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +10 ."nadia"Lysis1.seek :locked +4 "syringe1"C@( +249 "core" ( +3 "valve0"DL% +7 "core" %8[1O; +2 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +5 ."Plunge"Plunge.seek :locked +2 "valve0"DM'Z +7 "syringe1"CO% +7 "core" %ݮxp +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CQ'6 +69 "core" ' +36 ' +2 ."nadia"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 FP% +3 "core" %UdS  +7 #"nadia"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."nadia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +8 ."nadia"Gate.power :cartridge,:OFF +5 #Mounted archive Cartridge +2228 "Can"mh}-z@t;|{ +796 #"nadia"-> Cart.unlock +6 "valve1"EN& +3 "core" &O` o +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 FK% +3 "core" %p< \  +3 ."nadia"Carousel.home.seek 1 +3 "carousel0"FY' +17 "core" ' +2 "carousel0"FX% +8 "core" %O  +3 "syringe0"BW& +4 "core" &jؗ S +3 "syringe1"C`% +4 "core" %6 W +5 ."Air"Air.in :unlocking +3 "syringe0"Bb% +3 "syringe1"C> P]z-2 +7 "core" %j٢ +4 ."Air"Air.seek :clear +3 "syringe1"Cc' +94 "core" ' +4 "syringe1"Cd& +4 "core" &) +5 ."Lysis1"Lysis1.in :unlocking +2 "valve0"DU% +6 "syringe1"C? P[(2 +8 "core" %ZGOT +4 ."Lysis1"Lysis1.seek :clear +5 "syringe1"Ce( +5 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DV'8 +96 "core" ' +19 ( +156 #"nadia"-> Cart.dry +2072 #-> fire lysis +6 "valve0"Df% +5 Dg& +5 "core" %8[1O\\ +3 "carousel0"Fh% +10 "syringe1"Ci% +4 "syringe0"Bj& +4 "syringe1"Ck& +5 "syringe0"Bl% +6 "valve1"Em& +25 "core" &1O\\ + %O  + %ݠ + &jة A &S + %j٣ + &OX f +11 ."clearToroid"Toroid.seek :clear +4 "valve1"ES& +5 "valve0"DT( +10 "core" &O\\ ` + ( +4 ."nadia"Air.in :default +2 "syringe1"C>d P]z<2 +6 ."nadia"Air.seek :clear +3 "syringe1"C>' +5 "core" þ' +3 ."nadia"Lysis1.in :default + "syringe1"C?d P[<2 +6 ."nadia"Lysis1.seek :clear +3 "syringe1"Cn( +5 "core" ( +4 ."nadia"Lysis2.seek :clear +2 "syringe0"Bo'j +8 "core" ' +2 ."nadia"Archive.seek :clear +3 "syringe0"Bp(j +5 "core" ( +3 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fr% +3 "core" %Ou  +3 ."nadia"Carousel.home.seek 1 +3 "carousel0"Fs' +18 "core" ' +2 "valve0"Dt% +4 "core" %8[8OQ +4 #"nadia"Selecting Cartridge 52 +3 "valve0"Dq% +7 "core" %8[=OM } +2 "syringe1"Cx& +4 "core" &P +3 "syringe1"Cv% +5 "core" %ݣ +46 "valve1"Ew& +5 "core" &OQ c +3 ."nadia"Carousel.seek 52 +3 "carousel0"Fy'1 +1063 "core" ' +4 "carousel0"Fz% +3 "core" %10۶ 2 +3 ."nadia"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C{& +3 "core" &[| +4 ."nadia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +15 ."nadia"Lysis1.seek :locked +4 "syringe1"Cu( +6 "core" ' 1P۵!  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 52 - 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi:@pastThresholdF: @voltagef14.935723122238587@a: @currentiU: @type2F:@outPressi!:\ @lostF: @inPressi:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni1: @pastRLSF: @tagi~: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + ' +240 ( +2 "valve0"D}% +12 "core" %8[HOS r +2 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +3 "syringe1"C~% +7 "valve0"D'Z +3 "core" %ݤn +4 ."Air"Air.in :fast + "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +4 "syringe1"C|'6 +77 "core" ' +27 ' +3 ."nadia"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F % +3 "core" Ɖ%1UR  +7 #"nadia"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."nadia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."nadia"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."nadia"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" t +5 "cartridge" t +3 "Cartridge" \ y +5 "cartridge" yVV +3 "Cartridge" y  +3 "cartridge" y 1&0J59981 4&0{C3 +4 #"nadia"Mounted lysis Cartridge board#J59981 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"D% +4 "core" Ć%ZGrOY +3 "syringe1"C& +3 "core" Ä&u +3 "syringe1"C% +5 "core" Á%6h P +2 "carousel0"F% +5 "core" ƅ%1 \  +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +5 "valve0"D % +5 "core" ċ%ZG{OT +3 "syringe1"C& +5 "core" Ó&p +2 "syringe1"C% +4 "core" Ã%6x L +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +7 "core" Ǖ&J +43 "carousel1"G& +3 "core" Ǘ& +47 "carousel1"G& +3 "core" ǘ& +47 "carousel1"G& +3 "core" ǖ& +47 "carousel1"G& +3 "core" LJ&E +47 "carousel1"G & +3 "core" nj&c +47 "carousel1"G& +5 "core" Ǒ& +45 "carousel1"G & +3 "core" Ǎ& +48 "carousel1"G& +3 "core" Ǐ& +47 "carousel1"G& +5 "core" ǒ& +45 "carousel1"G& +3 "core" ǐ& +47 "carousel1"G& +3 "core" Ǚ& +47 "carousel1"G& +3 "core" ǜ& +47 "carousel1"G& +3 "core" Ǜ& +4 "Can"mh}-z@tf <9 + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 25C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +6 .PhaseSeparator.in :filling +2 "carousel1"G? +5 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +4 "core" ǝ, +4 ."FILTERING"Gate.power :sampler,:ON +706 "core" ǝ,}K +7 #"FILTERING"Head pressure reached 3.37psi in 8 seconds +3 ."sealing"Air.in :default +7 "carousel0"F% +2 "syringe1"C>d P]z<2 +5 "core" ƚ%1I \  +5 ."sealing"Air.seek :sealed +3 #"FILTERING"Priming sample loop w/20ml +5 "syringe1"C' +6 ."FILTERING"SamplePump.setPosition! 0ml +6 "sampler0"H7 +5 ."FILTERING"Exhaust.close +3 "sampler0"H&0 +147 "core" Ȧ0 +2 ."FILTERING"Bypass.open +2 "sampler1"I1 +103 "core" Þ' +47 ɔ1 +73 "ambient0"J.% +3 "core" ʮ%# +6 "Can"mh}-z@s0~=) +3 "ambient0"J!% +5 "core" ʡ%v% +3 ."FILTERING"Exhaust.open +2 "sampler0"H2 +143 "core" ȟ2 +73 ."closeBypass"Bypass.close +4 "sampler1"I(/ +147 "core" ɨ/ +73 "sampler0"H0% +3 "core" Ȱ%R +3 "ambient0"J1% +4 "core" ʱ%g +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J#+ +5 "core" ʣ+ +2 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?! +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G4, +5 "core" Ǵ, +3 "ambient0"J5% +54 "core" ʵ%n +3 "sampler0"H6% +5 "core" ȶ%yT +4 "sampler0"H$% +4 "core" Ȥ%U +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "11:25:06.92" +3936 "Can"jhO-z1s.?{ +346 jhO-z1s.?Y +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }l#(L@3 22Q +12 ."FILTERING"SamplePump.seek 20ml,1:50 +3 "sampler0"H8'm +52 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }l#(L@3 22Q +2601 "core" ȸ' +2 "sampler0"H2% +4 "core" Ȳ%m‹ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"I31 +151 "core" ɳ1 +2 ."FILTERING"Intake.close +2 "sampler0"H9/ +147 "core" ȹ/ +2 "carousel0"Fa% +5 "core" %1F \  +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :crack +4 "valve0"D:( +168 "core" ĺ( +77 ."FILTERING"Toroid.seek :cartridge +3 "valve0"D"(m +207 "core" Ģ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :clear +3 "valve0"D%( +372 "core" ĥ( +2 "ambient0"J% +5 "core" ʎ%z +3 "Can"jhO-z1s;BM +2 "ambient0"JE% +4 "core" %u0 +3 "ambient0"JF% +4 "core" % +3 #"FILTERING"Pressurizing cartridge to 14psi above 15.3psia +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Intake.open +2 "sampler0"HI1 +144 "core" 1 +3 ."FILTERING"Bypass.close +3 "sampler1"I]/ +151 "core" / +2 ."FILTERING"Exhaust.close +2 "sampler0"HH0 +145 "core" 0 +3 "sampler0"HR% +4 "core" %K +3 "ambient0"J\\% +4 "core" %" +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +7 ."FILTERING"Abs.hold + "ambient0"J + +6 "core" ʠ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G', +7 "core" ǧ, +3 "sampler0"H-% +6 "core" ȭ%f +2 "sampler0"H*% +5 "core" Ȫ%} +3 ."FILTERING"SamplePump.in :charge + "sampler0"H>lu0}3 22Q +7 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +3 "sampler0"H,' +1201 "core" Ȭ'V ɿ 2 +5 "sampler0"H)% +4 "core" ȩ%V{ 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"Intake.close +3 "sampler0"H=/ +145 "core" Ƚ/ +2 "ambient0"J<% +5 "core" ʼ% +2 #"FILTERING"Pressurized cartridge with 0.609ml to 83.8psi + #Isolated @24.1psia (8.9psi) +7 #Verifying cartridge holds at least 0.65..0.85 of 24.1psia +5 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +3 "ambient0"J;% +7 "core" ʻ% +44 "ambient0"JJ% +3 "core" % +47 "ambient0"JA% +5 "core" % +45 "ambient0"JG% +3 "core" % +47 "ambient0"J?% +3 "core" ʿ% +47 "ambient0"JD% +3 "core" %; +47 "ambient0"JL% +6 "core" % +44 "ambient0"JO% +3 "core" % +47 "ambient0"JB% +3 "core" % +47 "ambient0"JP% +5 "core" % +3 #"FILTERING"Cartridge pressure fell 1% to 23.8psia +2 "carousel0"FC% +6 "core" %1G \  +3 #"FILTERING"Sampling 1000.0ml +3 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"H+2 +146 "core" ȫ2 +3 ."FILTERING"Intake.open +2 "sampler0"HN1 +144 "core" 1 +4 "Can"jhO-z1s>?C + "ambient0"JK% +5 "core" %T +4 "ambient0"JX% +4 "core" %V +2 ."FILTERING"Abs.in :default +3 "ambient0"J>:. e  +6 ."FILTERING"Abs.hold +2 "ambient0"JW+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G`, +6 "core" , +2 "ambient0"Jb% +5 "core" % +3 "sampler0"Hd% +5 "core" %l  +2 "sampler0"HU% +49 "core" %ku  +19 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ l#(@@3 22Q +8 ."FILTERING"SamplePump.seek 1000ml,56:04 +3 "sampler0"H/'g+ +56 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d l#(@@3 22Q +225 HQ% +3 "core" %\ ^  2 +4 "ambient0"J@% +5 "core" %X + #"sampling"Cartridge 52 filtering 2/1000ml at 0.70ml/s with 11.4psi vacuum +4556 "Can"jh-"zOsIG{ +1437 "sampler0"HM% +3 "core" % "h 2 +3 "ambient0"Jf% +4 "core" % +2 #"sampling"Cartridge 52 filtering 45/1000ml at 0.73ml/s with 12.7psi vacuum +5988 "sampler0"Hm% +4 "core" % ,& 2 +3 "ambient0"Jl% +4 "core" %^ +2 #"sampling"Cartridge 52 filtering 89/1000ml at 0.73ml/s with 13.7psi vacuum +4512 "Can"mg-1z2suJ1P+{ +1474 "sampler0"Hh% +3 "core" % 'zh 2 +3 "ambient0"Jk% +4 "core" % +3 #"sampling"Cartridge 52 filtering 132/1000ml at 0.72ml/s with 11.8psi vacuum +5988 "sampler0"Hi% +5 "core" % &+8 2 +3 "ambient0"Jj% +4 "core" %t4 +2 #"sampling"Cartridge 52 filtering 176/1000ml at 0.72ml/s with 12.9psi vacuum +4471 "Can"kg- h 2 +2 "ambient0"Jx% +3 "core" %p +3 #"sampling"Cartridge 52 filtering 304/1000ml at 0.71ml/s with 14.4psi vacuum +741 #"espclient"-> exit +3 #Session Ended +783 #"beni"Session Begun +744 #-> hi +3716 "sampler0"Hv% +3 "core" %n   2 +3 "ambient0"Jw% +5 "core" %s +2 #"sampling"Cartridge 52 filtering 347/1000ml at 0.71ml/s with 14.7psi vacuum +4393 "Can"mh-Rz6sIwi0{ +1594 "sampler0"HZ% +3 "core" %Y, *ړ 2 +3 "ambient0"Jc% +5 "core" %x +2 #"sampling"Cartridge 52 filtering 390/1000ml at 0.71ml/s with 14.8psi vacuum +5987 "sampler0"He% +3 "core" % B ! 2 +3 "ambient0"J^% +4 "core" %N +2 #"sampling"Cartridge 52 filtering 433/1000ml at 0.71ml/s with 11.0psi vacuum +4355 "Can"lhI-`z1s{I`rN{ +1633 "sampler0"H_% +3 "core" %\ . $p 2 +2 "ambient0"JY% +4 "core" %} +3 #"sampling"Cartridge 52 filtering 476/1000ml at 0.72ml/s with 11.2psi vacuum +5988 "sampler0"Hs% +3 "core" %  H/ 2 +4 "ambient0"Jz% +4 "core" % +2 #"sampling"Cartridge 52 filtering 519/1000ml at 0.73ml/s with 11.1psi vacuum +4314 "Can"mh-dz3s|J{%l{ +1673 "sampler0"H{% +3 "core" % ,R 2 +3 "ambient0"J}% +5 "core" %x +2 #"sampling"Cartridge 52 filtering 563/1000ml at 0.73ml/s with 6.6psi vacuum 5.6m deep (22.9psia) +5987 "sampler0"H~% +3 "core" % 'wT 2 +3 "ambient0"Jn% +5 "core" % +2 #"sampling"Cartridge 52 filtering 606/1000ml at 0.72ml/s with 10.5psi vacuum 4.1m deep (20.7psia) +4272 "Can"nh-oz2sI{ +1715 "sampler0"H % +3 "core" ȉ% 3oc 2 +3 "ambient0"JV% +3 "core" % +3 #"sampling"Cartridge 52 filtering 650/1000ml at 0.72ml/s with 8.0psi vacuum +5988 "sampler0"H>% +5 "core" Ⱦ%E (j 2 +3 "ambient0"J% +3 "core" ʂ% +3 #"sampling"Cartridge 52 filtering 693/1000ml at 0.72ml/s with 10.1psi vacuum +4230 "Can"miB-pz5sIu{ +1755 "sampler0"H\ % +3 "core" Ȋ%J ! 2 +3 "ambient0"J% +4 "core" ʈ%E +3 #"sampling"Cartridge 52 filtering 736/1000ml at 0.72ml/s with 8.3psi vacuum 4.0m deep (20.6psia) +5988 "sampler0"H% +4 "core" Ȇ%k ;' 2 +2 "ambient0"J% +5 "core" ʄ% +2 #"sampling"Cartridge 52 filtering 779/1000ml at 0.72ml/s with 11.5psi vacuum +4191 "Can"ki-vz2sxJZ{ +1796 "sampler0"H% +5 "core" ȁ% 1 2 +2 "ambient0"J% +4 "core" ʅ%X +2 #"sampling"Cartridge 52 filtering 822/1000ml at 0.71ml/s with 10.8psi vacuum +5987 "sampler0"H % +3 "core" ȋ% < 2 +3 "ambient0"J% +5 "core" ʓ%c +2 #"sampling"Cartridge 52 filtering 864/1000ml at 0.71ml/s with 8.3psi vacuum +4152 "Can"mi-z1syI{ +1835 "sampler0"H% +3 "core" ȃ%i t3s 2 +2 "ambient0"J% +5 "core" ʕ% +2 #"sampling"Cartridge 52 filtering 907/1000ml at 0.71ml/s with 10.7psi vacuum +5988 "sampler0"H% +3 "core" ȗ%P " 2 +3 "ambient0"J% +4 "core" ʘ%bI +2 #"sampling"Cartridge 52 filtering 949/1000ml at 0.70ml/s with 13.4psi vacuum +4112 "Can"lj\ -z2sI8i{ +1875 "sampler0"H% +4 "core" Ȗ%7\ #b 2 +3 "ambient0"J% +3 "core" ʇ%Q +3 #"sampling"Cartridge 52 filtering 991/1000ml at 0.70ml/s with 10.1psi vacuum +1251 "core" ȯ' +2 "sampler0"H % +5 "core" Ȍ%g+ 2 +2 ."FILTERING"Abs.stop +3 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +13 ."closeIntake"Intake.close +3 "sampler0"H/ +4 ."closeExhaust"Exhaust.close +7 "sampler0"H 0 +7 "carousel0"F% +10 "core" Ə%1vH \  +7 ."FILTERING"Toroid.in :hold +3 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"D( +109 "core" ȑ/ +9 ȍ0 +238 Ē( +6 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +9 ."FILTERING"Toroid.seek :clear +2 "valve0"D( +355 "core" Đ( +4 #"FILTERING"Sampled 1000.0ml +14 "carousel0"F% +4 "core" ƙ%1IE \  +10 "valve0"D% +4 "core" Ĝ%ZGkO< +3 "syringe1"C& +4 "core" Û&w +3 "syringe1"C% +4 "core" Ú%p +3 "carousel0"F&% +5 "core" Ʀ%1H \  +3 "carousel0"F|% +57 "core" %1H \  +5 ."PROCESSING"Handoff.select :waste +3 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C% +7 "core" Ô%s +43 "syringe1"C.% +3 "core" î%qV +47 "syringe1"C!% +3 "core" á%q +46 ."sourcePumpShutdown"Gate.power :sampler,:OFF +7 "syringe1"C% +3 "core" ß%v +41 "syringe1"C(% +3 "core" è%y} +47 "syringe1"C0% +3 "core" ð%ux +47 "syringe1"C1% +3 "core" ñ%s +47 "syringe1"C5% +3 "core" õ%p +47 "syringe1"C6% +4 "core" ö% +46 "syringe1"C$% +5 "core" ä%p +45 "syringe1"C% +4 "core" Ý%r +46 "syringe1"C7% +3 "core" ÷%s +47 "syringe1"C2% +3 "core" ò%t +47 "syringe1"C3% +3 "core" ó%v +47 "syringe1"C#% +3 "core" ã%mg +47 "syringe1"C4% +3 "core" ô%qv +47 "syringe1"C9% +3 "core" ù%l +47 "syringe1"Ca% +4 "core" %j +46 "syringe1"CT% +3 "core" %l +47 "syringe1"C:% +5 "core" ú%n +45 "syringe1"C"% +3 "core" â%k +47 "syringe1"C% +3 "core" Î%i +47 "syringe1"CE% +3 "core" %q +47 "syringe1"CF% +3 "core" %o +47 "syringe1"CI% +3 "core" %g +47 "syringe1"C]% +4 "core" %i +46 "syringe1"CH% +4 "core" %h +46 "syringe1"CR% +3 "core" %h +47 "syringe1"C\\% +3 "core" %o +5 "Can"lj\ -z2tc vb + #"PROCESSING"Syringe tare is 50% of full scale at 26C (drifted -1%) +4 .PinPort.in :locking +5 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"E-( N +81 "core" ŭ( +3 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 1250uL +6 ."posArchive"Archive.seek :empty +6 "syringe0"B>d P\\k<2 +7 "syringe1"C*( +2 "syringe0"B8( +8 ."posLyS2"Lysis2.seek :empty +5 "syringe0"B)' +239 "core" ê( +585 ¸( +10 ©' +2 "carousel0"F=% +4 "core" ƽ%1+v {\ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +4 .PinPort.seek :locked +5 "valve1"E<( N +7 "core" ż( +2 ."PROCESSING"Handoff.select :waste +5 .Plunge.seek :puckBottom +8 "valve0"D;'B +129 "core" Ļ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CJ% +9 "core" % +41 "syringe1"CA% +3 "core" % +47 "syringe1"C % +3 "core" à% +47 "syringe1"C'% +3 "core" ç% +47 "syringe1"CG% +4 "core" % +46 "syringe1"C?% +3 "core" ÿ% +47 "syringe1"CD% +3 "core" % +47 "syringe1"CL% +5 "core" % +46 "syringe1"CO% +3 "core" % +47 "syringe1"CB% +3 "core" % +47 "syringe1"CP% +3 "core" % +47 "syringe1"CC% +4 "core" % +46 "syringe1"C+% +5 "core" ë% +45 "syringe1"CN% +4 "core" % +46 "syringe1"CK% +3 "core" % +47 "syringe1"CX% +3 "core" % +47 "syringe1"Cb% +3 "core" % +47 "syringe1"Cd% +5 "core" % +45 "syringe1"CU% +4 "core" % +46 "syringe1"CQ% +4 "core" % +46 "syringe1"C@% +3 "core" % +47 "syringe1"C,% +3 "core" ì% +47 "syringe1"CM% +3 "core" %M +47 "syringe1"Cf% +3 "core" %8 +47 "syringe1"Cm% +3 "core" % +5 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi + #Air stroke #1 of 1 +5 "syringe1"Cl% +8 "core" % +3 ."PROCESSING"Air.in :pressurize + "syringe1"C>@N y P]z<2 +6 ."PROCESSING"Air.seek :pressurized @1785340380.29EDT = =tMAIN =mMAIN_scheduler @EDT1785340381.50 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +147 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +29 "Can"ok-z:to D? +341 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +68 #Sea pressure (Abs) tare is 6% of full scale +245 "Can"ok-z:ta sWA +2 ok-z:ta sWA + #tPolling Can every 2:00 +4 #Waiting for espclients... +47 "Can"mk!-z1tt 0YA +1683 #"andia"Session Begun +1739 #-> slots +1424 #-> Cart.dry +420 #-> SC +3 "carousel0"F% +5 "core" Ɓ%1 \ +3032 #"andia"-> slot 52=>[:dry] +783 #-> Cart.dry +2866 "Can"kk-z1tt (_ +2151 #"andia"-> BACsampler.goalVolume +1720 #-> BACsampler.goalVolume=100 +5360 #-> lysis=:lyseHold_phyto +2723 "Can"nl-|z3tr } +2029 #"andia"-> start {fire lysis} +37 ."detached from andia"Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +51 "valve0"DP +3 D +3 "core" Ă +7 "valve0"D% +3 "core" ă%B(JOJ +3 ."detached from andia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from andia"Plunge.hold +2 "valve0"D+ +5 "core" Ą+ +2 ."detached from andia"Plunge.configure :default +3 "valve0"D# P ޤ-2 +8 "sampler0"H% +3 "core" ȅ%g+E +3 ."detached from andia"SamplePump.configure :default +3 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" Ć&OO ! +3 ."detached from andia"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from andia"Toroid.hold +2 "valve0"D, +5 "core" ć, +2 ."detached from andia"Toroid.configure :default +3 "valve0"D$ ֜2 +8 "carousel0"F% +4 "core" ƈ%1 \ +3 ."detached from andia"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from andia"Carousel.hold +3 "carousel0"F + +4 "core" Ɖ+ +3 ."detached from andia"Carousel.configure :default +2 "carousel0"F#<` F 22 +11 "ambient0"J\ % +6 "core" ʊ% +3 ."detached from andia"Abs.configure :default +28 "ambient0"J#  +9 "carousel1"G & +3 "core" Nj&Oy +3 ."detached from andia"PhaseSeparator.configure :default +2 "carousel1"G$ +8 "syringe0"B & +4 "core" Œ&*p! * +3 ."detached from andia"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from andia"Archive.hold +2 "syringe0"B , +5 "core" , +3 ."detached from andia"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 "syringe1"C% +4 "core" Î%{ +3 ."detached from andia"Air.configure :default + "syringe1"C#d P]z<2 +6 ."detached from andia"Air.hold +2 "syringe1"C+ +5 "core" Ï+ +3 ."detached from andia"Air.configure :default + "syringe1"C#d P]z<2 +7 C& +3 "core" Ð&6 L +3 ."detached from andia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from andia"Lysis1.hold +2 "syringe1"C, +5 "core" Ñ, +3 ."detached from andia"Lysis1.configure :default +3 "syringe1"C$d P[<2 +5 "syringe0"B% +4 "core" ’%*8 +3 ."detached from andia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from andia"Lysis2.hold +2 "syringe0"B+ +4 "core" “+ +3 ."detached from andia"Lysis2.configure :default +29 "syringe0"B#d P\\k<2 +9 "valve1"E& +3 "core" Ŕ& N;OK +3 ."detached from andia"PinPort.configure :default +2 "valve1"E$ B22 +6 ."detached from andia"PinPort.hold +2 "valve1"E, +6 "core" ŕ, +3 ."detached from andia"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from andia"All elf controllers running firmware version 4.94 +107 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +4 !"detached from andia"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'";/home/esp/esp2/utils/auv/cmd.rb:255:in `loadCartridge'",/home/esp/esp2/lib/msg.rb:68:in `retry'";/home/esp/esp2/utils/auv/cmd.rb:253:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"!andia:9:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +5 "valve0"D% +5 "core" Ė%B((OE +3 "valve0"D& +4 "carousel0"F% +7 "syringe0"B& +10 "syringe1"C% +3 C& +3 "syringe0"B% +7 "valve1"E& +17 "core" ė&OB / + Ƙ%1P  + ™&*ny , Ú%6 + Û&: K + œ%*< + ŝ& N;OE +9 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +4 "valve0"D( +8 "core" Ş& N;OB + ğ( +4 ."detached from andia"Air.seek :clear +2 "syringe1"C ' +470 "core" à' +5 ."detached from andia"Lysis1.seek :clear +3 "syringe1"C!( +773 "core" á( +2 ."detached from andia"Lysis2.seek :clear +3 "syringe0"B"'j +1855 "core" ¢' +2 ."detached from andia"Archive.seek :clear +5 "syringe0"B#(j +1854 "core" £( +2 ."detached from andia"Carousel.in :default +2 "carousel0"F><` F 22 +7 F$% +4 "core" Ƥ%1Sj  +3 ."detached from andia"Carousel.home.seek 52 +3 "carousel0"F%'1 +16 "core" ƥ' +2 "valve0"D&% +5 "core" Ħ%B(?OV +2 "syringe1"C'& +3 "core" ç&\\r +5 #"detached from andia"Selecting Cartridge 22 + "valve0"D(% +8 "core" Ĩ%B(JOS +4 ."detached from andia"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E)& +4 "core" ũ& N;OV +4 ."detached from andia"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from andia"PinPort.seek :clear +3 "valve1"E*( +80 "core" Ū( +3 ."detached from andia"Carousel.in :default +2 "carousel0"F><` F 22 +44 F+% +3 "core" ƫ%1P  +13 ."detached from andia"Carousel.home.seek 52 +3 "carousel0"F,'1 +17 "core" Ƭ' +2 "carousel0"F-% +4 "core" ƭ%1O\  +3 "syringe0"B.& +5 "core" ®&jؕx U +2 "syringe1"C/% +5 "core" ï%ݤp +2 "syringe0"B0% +5 "core" °%jْv +2 "syringe1"C1& +4 "core" ñ&\\Ѐ{ +3 "valve0"D2% +5 "core" IJ%B(?OV +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +2 "valve0"D3'8 +181 "core" ij' +3 ."detached from andia"Carousel.seek 22 +4 "carousel0"F4' +636 "core" ƴ' +4 "carousel0"F5% +3 "core" Ƶ%=[ 2 +3 ."detached from andia"Carousel.reconfigure :align + "carousel0"F#` F 22 +9 "syringe1"C6& +5 "core" ö&MȀ +4 ."detached from andia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from andia"Lysis1.seek :locked +3 "syringe1"C7( +249 "core" ÷( +3 "valve0"D8% +8 "core" ĸ%8[6O. +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +8 ."Plunge"Plunge.seek :locked +4 "syringe1"C9% +4 "valve0"D:'Z +7 "core" ù%ݩ +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"C;'6 +76 "core" ĺ' +28 û' +3 ."detached from andia"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +8 F<% +2 "core" Ƽ%][RO  +7 #"detached from andia"Powering cartridge bus +263 "cartridge_broadcast" ( +102 ."detached from andia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from andia"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."detached from andia"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" =t +5 "cartridge" t +3 "Cartridge" >y +5 "cartridge" y]'6(i +3 "Cartridge" ?y 5 +4 "cartridge" y ?"UC\\q A"J60003 "dCb Bm/GyCg +4 #"detached from andia"Mounted lysis Cartridge board#J60003 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"D@% +4 "core" %ZGOO[ +3 "syringe1"CA& +4 "core" &  +3 "syringe1"CB% +4 "core" %6 Z +2 "carousel0"FC% +4 "core" %[ \ +4 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 22 +3 "valve0"DD% +8 "core" %ZGRO[ \ +2 "syringe1"CE& +5 "core" &퀰 +2 "syringe1"CF% +4 "core" %6 c +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +3 "carousel1"GG& +6 "core" & +44 "carousel1"GH& +3 "core" & +47 "carousel1"GI& +3 "core" & +47 "carousel1"GJ& +3 "core" &8 +47 "carousel1"GK& +3 "core" & +47 "carousel1"GL& +3 "core" & +47 "carousel1"GM& +3 "core" &{ +47 "carousel1"GN& +4 "core" & +47 "carousel1"GO& +3 "core" & +47 "carousel1"GP& +4 "core" & +46 "carousel1"GQ& +3 "core" &? +47 "carousel1"GR& +3 "core" & +47 "carousel1"GS& +3 "core" & +47 "carousel1"GT& +3 "core" &x +9 "Can"nl-|z3tn  + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) +3 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +6 .PhaseSeparator.in :filling + "carousel1"G? +5 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GU, +5 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +699 "core" ,JR +7 #"FILTERING"Head pressure reached 3.31psi in 8 seconds +4 ."sealing"Air.in :default +4 "syringe1"C>d P]z<2 +4 "carousel0"FV% +5 "core" %[I \ +2 ."sealing"Air.seek :sealed +8 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +2 "syringe1"CW' +2 ."FILTERING"SamplePump.setPosition! 0ml +6 "sampler0"H7 +5 ."FILTERING"Exhaust.close +3 "sampler0"HX0 +8 "core" 0 +2 ."FILTERING"Bypass.open +2 "sampler1"IY1 +150 "core" 1 +73 "ambient0"JZ% +4 "core" %  +3 ."FILTERING"Intake.open + "sampler0"H[1 +13 "core" ' +129 1 +2 "ambient0"J\\% +6 "core" %z +3 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +56 "sampler0"H]% +9 "core" % +41 "sampler0"H^% +3 "core" %v +47 "sampler0"H_% +3 "core" %p +47 "sampler0"H`% +4 "core" %p +46 "sampler0"Ha% +3 "core" %m +47 "sampler0"Hb% +3 "core" %e +5 "Can"nl-|z3s0 +2 #"FILTERING"Vacuum tare is 53% of full scale at 26C (drifted 2%) + "Can"ll-z3s0~ +3 "ambient0"Jc% +6 "core" %s +2 "ambient0"Jd% +5 "core" %L +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"Je+ +6 "core" + +3 ."FILTERING"PhaseSeparator.in :full +3 "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"Gf, +6 "core" , +4 "sampler0"Hg% +4 "core" %a +3 "sampler0"Hh% +3 "core" %k +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "11:59:54.69" +4467 "Can"ll-z3s0r +4 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m9#(@4r3 22Q +12 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"Hi'6 +52 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m9#(@4r3 22Q +1290 "core" ' +4 "sampler0"Hj% +5 "core" %6 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +6 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"Hk2 +145 "core" 2 +73 ."closeBypass"Bypass.close +4 "sampler1"Il/ +152 "core" / +74 "sampler0"Hm% +3 "core" %5 +3 "ambient0"Jn% +3 "core" % +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +7 ."FILTERING"Abs.hold +4 "ambient0"Jo+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gp, +7 "core" , +3 "ambient0"Jq% +5 "core" % +3 "sampler0"Hr% +4 "core" %1 +2 "sampler0"Hs% +5 "core" % +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m9#(@r3 22Q +8 ."FILTERING"SamplePump.seek 200ml,13:50 +3 "sampler0"Ht'G +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m9#(@r3 22Q +5428 "Can"ml-z0sI? +11958 ll-zFsI׳ +8833 "core" ' +2 "sampler0"Hu% +4 "core" %Gn 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +11 ."FILTERING"Bypass.open +2 "sampler1"Iv1 +153 "core" 1 +2 ."FILTERING"Intake.close +3 "sampler0"Hw/ +145 "core" / +2 "carousel0"Fx% +4 "core" %[J \ +7 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +9 ."FILTERING"Toroid.seek :crack +5 "valve0"Dy( +170 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"Dz(m +206 "core" ( +5 ."FILTERING"Toroid.in :default +3 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :clear +3 "valve0"D{( +372 "core" ( +2 "ambient0"J|% +4 "core" %j" +4 "Can"ll-zFs;x +2 "ambient0"J}% +3 "core" %p) +3 "ambient0"J~% +3 "core" %l) +4 #"FILTERING"Pressurizing cartridge to 14psi above 15.5psia +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +7 ."FILTERING"Intake.open +2 "sampler0"H1 +141 "core" 1 +5 ."FILTERING"Bypass.close +2 "sampler1"I/ +149 "core" Ɂ/ +2 ."FILTERING"Exhaust.close +2 "sampler0"H0 +155 "core" Ȃ0 +3 "sampler0"H% +4 "core" ȃ%m +2 "ambient0"J% +3 "core" ʅ%`\ +4 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J+ +5 "core" ʆ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +7 "core" Lj, +3 "sampler0"H\ % +5 "core" Ȋ% +6 "sampler0"H % +3 "core" ȋ% +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>m9u0@3 22Q +8 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +4 "sampler0"H ' +1321 "core" Ȍ' 2 +15 "sampler0"H% +3 "core" Ȏ%1  +3 ."FILTERING"Abs.stop +3 "Can"lm-z6s9N- +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +7 ."FILTERING"Intake.close +2 "sampler0"H/ +143 "core" Ȑ/ +3 "ambient0"J% +4 "core" ʒ% +2 #"FILTERING"Pressurized cartridge with 0.690ml to 14.1psi +2 #Isolated @28.4psia (12.9psi) +5 #Verifying cartridge holds at least 0.65..0.85 of 28.4psia +7 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +6 "ambient0"J% +6 "core" ʔ% +44 "ambient0"J% +3 "core" ʖ% +47 "ambient0"J% +3 "core" ʝ% +47 "ambient0"J% +3 "core" ʜ% +47 "ambient0"J% +3 "core" ʛ% +47 "ambient0"J% +3 "core" ʚ% +47 "ambient0"J% +5 "core" ʙ% +45 "ambient0"J% +3 "core" ʘ% +47 "ambient0"J% +3 "core" ʗ% +47 "ambient0"J% +3 "core" ʞ% +3 #"FILTERING"Cartridge pressure fell 1% to 28.0psia +2 "carousel0"F% +8 "core" Ƈ%[Jk \ +3 #"FILTERING"Sampling 1000.0ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +5 ."FILTERING"Exhaust.open +3 "sampler0"H2 +150 "core" ȏ2 +9 ."FILTERING"Intake.open +5 "sampler0"H1 +141 "core" ȑ1 +4 "Can"lm-z6s=˾) +2 "ambient0"J% +3 "core" ʓ%6 +6 "ambient0"J % +3 "core" ʍ%E +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"J$+ +6 "core" ʤ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G&, +8 "core" Ǧ, +3 "ambient0"J'% +5 "core" ʧ%( +4 "sampler0"H(% +4 "core" Ȩ%u  +3 "sampler0"H)% +6 "core" ȩ%{  +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ m9#(@@@3 22Q +7 ."FILTERING"SamplePump.seek 1000ml,56:04 +5 "sampler0"H+'g+ +55 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d m9#(@@@3 22Q +226 H-% +3 "core" ȭ% N 2 +3 "ambient0"J.% +5 "core" ʮ%x +2 #"sampling"Cartridge 22 filtering 2/1000ml at 0.63ml/s with 10.8psi vacuum +5993 "sampler0"H/% +3 "core" ȯ% 0 2 +3 "ambient0"J0% +3 "core" ʰ% +3 #"sampling"Cartridge 22 filtering 43/1000ml at 0.69ml/s with 7.6psi vacuum 4.2m deep (20.8psia) +4582 "Can"mm;-z3sIӠ +698 #"andia"-> PhytoSampler.goalVolume +424 #-> PhytoSampler.goalVolume=100 +283 "sampler0"H1% +3 "core" ȱ%Z ol 2 +3 "ambient0"J2% +4 "core" ʲ%xq +2 #"sampling"Cartridge 22 filtering 85/1000ml at 0.69ml/s with 7.4psi vacuum 5.0m deep (22.1psia) +2593 #"andia"-> slots 52=>nil +1056 #-> slot 52=>:dry +2339 "sampler0"H % +3 "core" ȉ%  2 +3 "ambient0"J% +5 "core" ʕ%\ +2 #"sampling"Cartridge 22 filtering 126/1000ml at 0.68ml/s with 11.4psi vacuum +4535 "Can"omd-z?sI>41 +1453 "sampler0"H%% +3 "core" ȥ% &y^ 2 +3 "ambient0"J% +4 "core" ʄ% +2 #"sampling"Cartridge 22 filtering 166/1000ml at 0.68ml/s with 11.1psi vacuum +5988 "sampler0"H,% +3 "core" Ȭ%l} (| 2 +3 "ambient0"J5% +3 "core" ʵ% +3 #"sampling"Cartridge 22 filtering 207/1000ml at 0.67ml/s with 11.4psi vacuum +4491 "Can"lm-z2sInO +1497 "sampler0"H6% +3 "core" ȶ%F R 2 +3 "ambient0"J8% +5 "core" ʸ% +2 #"sampling"Cartridge 22 filtering 247/1000ml at 0.66ml/s with 11.1psi vacuum +5987 "sampler0"H9% +3 "core" ȹ% ,f 2 +3 "ambient0"J!% +3 "core" ʡ% +2 #"sampling"Cartridge 22 filtering 286/1000ml at 0.66ml/s with 9.0psi vacuum +4448 "Can"jm-z3syIEcm +1541 "sampler0"H<% +3 "core" ȼ%\ U 2 +3 "ambient0"J3% +4 "core" ʳ% +2 #"sampling"Cartridge 22 filtering 325/1000ml at 0.66ml/s with 11.1psi vacuum +5988 "sampler0"H % +3 "core" Ƞ%ʚ  2 +3 "ambient0"J=% +3 "core" ʽ%H +3 #"sampling"Cartridge 22 filtering 364/1000ml at 0.65ml/s with 7.8psi vacuum 4.4m deep (21.1psia) +4402 "Can"im-z3sxJ] +1586 "sampler0"H>% +3 "core" Ⱦ%F\ P 2 +3 "ambient0"J?% +4 "core" ʿ%y +2 #"sampling"Cartridge 22 filtering 402/1000ml at 0.64ml/s with 9.8psi vacuum +5988 "sampler0"H@% +3 "core" % n\ }6 2 +3 "ambient0"JA% +4 "core" %w +2 #"sampling"Cartridge 22 filtering 441/1000ml at 0.64ml/s with 9.7psi vacuum +4356 "Can"om-z4sH +1632 "sampler0"HB% +3 "core" %\ =H 0# 2 +3 "ambient0"JC% +3 "core" % +3 #"sampling"Cartridge 22 filtering 478/1000ml at 0.63ml/s with 9.5psi vacuum +5988 "sampler0"HD% +3 "core" % 1\ wt 2 +3 "ambient0"JE% +3 "core" % +2 #"sampling"Cartridge 22 filtering 516/1000ml at 0.63ml/s with 11.6psi vacuum +4313 "Can"nn--z1sHdz +1676 "sampler0"HF% +3 "core" % D . 2 +3 "ambient0"JG% +3 "core" % +2 #"sampling"Cartridge 22 filtering 553/1000ml at 0.62ml/s with 11.8psi vacuum +5989 "sampler0"HH% +3 "core" % |\ | 2 +3 "ambient0"JI% +3 "core" %c +3 #"sampling"Cartridge 22 filtering 591/1000ml at 0.62ml/s with 9.3psi vacuum +4267 "Can"knR-z=sIp +1721 "sampler0"HJ% +3 "core" % m` 9r\ 2 +3 "ambient0"JK% +3 "core" % +3 #"sampling"Cartridge 22 filtering 627/1000ml at 0.61ml/s with 13.3psi vacuum +5988 "sampler0"HL% +48 "core" %6"\ | 2 +3 "ambient0"JM% +13 "core" %k +2 #"sampling"Cartridge 22 filtering 664/1000ml at 0.61ml/s with 11.6psi vacuum +4172 "Can"ln}-z?s|J/  +1762 "sampler0"HN% +3 "core" % G  2 +3 "ambient0"JO% +5 "core" % + #"sampling"Cartridge 22 filtering 701/1000ml at 0.61ml/s with 12.8psi vacuum +5988 "sampler0"HP% +3 "core" %Ƶ 5' 2 +3 "ambient0"JQ% +5 "core" % +2 #"sampling"Cartridge 22 filtering 737/1000ml at 0.61ml/s with 11.8psi vacuum +4184 "Can"kn-z2sI ! +1803 "sampler0"HR% +3 "core" % $s5 2 +3 "ambient0"JS% +3 "core" % +3 #"sampling"Cartridge 22 filtering 774/1000ml at 0.61ml/s with 12.2psi vacuum +5988 "sampler0"HT% +3 "core" %ZD * 2 +3 "ambient0"JV% +4 "core" % +2 #"sampling"Cartridge 22 filtering 811/1000ml at 0.62ml/s with 9.1psi vacuum +4144 "Can"mn-z2sH)+? +1844 "sampler0"HX% +3 "core" %!\ b%C 2 +3 "ambient0"JY% +3 "core" %^ +3 #"sampling"Cartridge 22 filtering 847/1000ml at 0.60ml/s with 16.1psi vacuum +5060 #"andia"Session Ended +928 "sampler0"HZ% +3 "core" %\ v 2 +3 "ambient0"J;% +4 "core" ʻ%= +2 #"sampling"Cartridge 22 filtering 883/1000ml at 0.60ml/s with 18.0psi vacuum +4101 "Can"kn-z3sI1] +1887 "sampler0"H[% +5 "core" %\ Vp 2 +2 "ambient0"J\\% +3 "core" % +3 #"sampling"Cartridge 22 filtering 919/1000ml at 0.60ml/s with 9.4psi vacuum +5986 "sampler0"H]% +3 "core" %q D&s> 2 +3 "ambient0"J^% +4 "core" % +2 #"sampling"Cartridge 22 filtering 955/1000ml at 0.60ml/s with 8.4psi vacuum +4061 "Can"lo-z4sJ:U{ +1928 "sampler0"H_% +3 "core" %7N $v 2 +3 "ambient0"J`% +4 "core" % +2 #"sampling"Cartridge 22 filtering 991/1000ml at 0.60ml/s with 7.4psi vacuum +1436 "core" ȫ' +3 "sampler0"Ha% +4 "core" %g+ 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +10 ."closeIntake"Intake.close +5 "sampler0"Hb/ +4 ."closeExhaust"Exhaust.close +6 "carousel0"Fc% + "sampler0"Hd0 +5 "core" %[I \ +9 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :bypass +2 "valve0"Dg( +110 "core" / +19 0 +223 ( +5 ."FILTERING"Toroid.in :default +5 "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :clear +2 "valve0"Dh( +352 "core" ( +3 #"FILTERING"Sampled 1000.0ml +3 "carousel0"FU% +4 "core" %[,K \ +8 "valve0"Dj% +3 "core" %ZGOOH +3 "syringe1"Ck& +3 "core" &, +3 "syringe1"Ce% +5 "core" %t +4 "carousel0"Ff% +6 "core" %[J \ +4 "carousel0"Fl% +5 "core" %[GJ \ +4 ."PROCESSING"Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"Cm% +3 "core" % +48 "syringe1"Cn% +3 "core" % +47 "syringe1"Cq% +3 "core" % +47 "syringe1"Cr% +3 "core" % +47 "syringe1"Cs% +3 "core" % +5 ."sourcePumpShutdown"Gate.power :sampler,:OFF +42 "syringe1"Ci% +3 "core" % +47 "syringe1"Cu% +3 "core" % +47 "syringe1"Cv% +3 "core" % +48 "syringe1"Co% +3 "core" % +47 "syringe1"Cp% +4 "core" % +46 "syringe1"Cw% +3 "core" % +47 "syringe1"Cx% +4 "core" % +46 "syringe1"C% +3 "core" ß% +47 "syringe1"Cy% +3 "core" % +4 "Can"lo-z4ta =q + #"PROCESSING"Syringe tare is 50% of full scale at 26C (drifted -1%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"Ez( N +78 "core" ( +3 ."posArchive"Archive.in :fast +6 "syringe0"B?d PZ<2 +4 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1250uL +2 "syringe0"B>d P\\k<2 +2 ."posArchive"Archive.seek :empty +5 "syringe1"C|( +2 "syringe0"B}( +5 ."posLyS2"Lysis2.seek :empty +5 "syringe0"B~' +247 "core" ( +583 ( +5 ' +2 "carousel0"F% +4 "core" %+[ |\ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +2 .PinPort.seek :locked +2 "valve1"E( N +5 "core" Ł( +2 ."PROCESSING"Handoff.select :waste +2 .Plunge.seek :puckBottom +2 "valve0"D'B +126 "core" Ă' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"C% +5 "core" Ã% +46 "syringe1"C% +3 "core" Å%m +47 "syringe1"C\ % +4 "core" Ê% +48 "syringe1"C % +4 "core" Ë% +44 "syringe1"Ct% +3 "core" %} +47 "syringe1"C% +3 "core" Î%v +47 "syringe1"C% +3 "core" Ð% +48 "syringe1"C% +5 "core" Ò% +46 "syringe1"C% +5 "core" Ô% +45 "syringe1"C% +3 "core" Ö%y +47 "syringe1"C% +3 "core" Ý%n +48 "syringe1"C% +3 "core" Æ%b +46 "syringe1"C% +3 "core" È%\\ +47 "syringe1"C% +3 "core" Ü%h +47 "syringe1"C% +3 "core" Û%w +13 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi + #Air stroke #1 of 1 + "syringe1"C% +3 "core" Ú% +4 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +3 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C' +515 "core" Ù' +2 "syringe1"C% +3 "core" Ø%SɁV < +3 #"PROCESSING"1.0psi after pressurize +2 .Air.in :compress +2 "syringe1"C>N  P]zP2 +3 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +259 "core" ×'=pG vP +4 "syringe1"C% +5 "core" Þ%<-G  |P +2 #"PROCESSING"Reached 4.5 of 4.0psi after pumping 1551uL (0.87 strokes) + .Air.in :default +2 "syringe1"C>d P]z<2 +3 ."PROCESSING"Air.hold +2 "syringe1"C+ +3 "core" Ç+ +7 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"C% +5 "core" Ï%<G e +46 "syringe1"C% +3 "core" Ñ%<G. +47 "syringe1"C% +3 "core" Ó%<G +47 "syringe1"C % +3 "core" Í%<G" +47 "syringe1"C'% +4 "core" ç%<G +46 "syringe1"C(% +3 "core" è%<G +47 "syringe1"C)% +3 "core" é%<G$ +47 "syringe1"C-% +3 "core" í%<G~ +48 "syringe1"C.% +3 "core" î%<G +47 "syringe1"C % +3 "core" Ì%<G +47 "syringe1"C/% +3 "core" ï%<G +47 "syringe1"C0% +3 "core" ð%<G +47 "syringe1"C1% +3 "core" ñ%<G +47 "syringe1"C2% +3 "core" ò%<G1 +47 "syringe1"C % +3 "core" É%<Gd +47 "syringe1"C% +3 "core" Õ%<G +47 "syringe1"C%% +3 "core" å%<G +47 "syringe1"C% +3 "core" Ä%<G +47 "syringe1"C,% +3 "core" ì%<G +47 "syringe1"C5% +3 "core" õ%<G +47 "syringe1"C6% +3 "core" ö%<G +47 "syringe1"C8% +3 "core" ø%<G +47 "syringe1"C9% +3 "core" ù%<G +47 "syringe1"C!% +3 "core" á%<G +47 "syringe1"C<% +4 "core" ü%<G% +46 "syringe1"C3% +3 "core" ó%<G@ +47 "syringe1"C % +3 "core" à%<Gl +47 "syringe1"C=% +3 "core" ý%<G} +47 "syringe1"C>% +4 "core" þ%<G  +46 "syringe1"C?% +3 "core" ÿ%<G  +47 "syringe1"C@% +3 "core" %<G +47 "syringe1"CA% +3 "core" %<G +47 "syringe1"CB% +3 "core" %<G +47 "syringe1"CC% +3 "core" %<G +47 "syringe1"CD% +4 "core" %<G +46 "syringe1"CE% +3 "core" %<G +47 "syringe1"CF% +3 "core" %<G +47 "syringe1"CG% +3 "core" %<G +47 "syringe1"CH% +3 "core" %<G +47 "syringe1"CI% +3 "core" %<G +47 "syringe1"CJ% +3 "core" %<G +47 "syringe1"CK% +3 "core" %<G +47 "syringe1"CL% +3 "core" %<G +47 "syringe1"CM% +3 "core" %<G}! +47 "syringe1"CN% +4 "core" %<G~4 +46 "syringe1"CO% +3 "core" %<G~B +47 "syringe1"CP% +3 "core" %<G] +47 "syringe1"CQ% +3 "core" %<G\\ +47 "syringe1"CR% +3 "core" %<G~] +47 "syringe1"CS% +3 "core" %<G|X +47 "syringe1"CT% +3 "core" %<G~c +47 "syringe1"CV% +3 "core" %<G za +47 "syringe1"CX% +44 "core" %<Gh +6 "syringe1"CY% +3 "core" %<Gxt +47 "syringe1"CZ% +4 "core" %<Gx_ +46 "syringe1"C;% +3 "core" û%<Gtt +47 "syringe1"C[% +2 "core" %<Gzt +3 #"PROCESSING"Stabilized at 13.6psi after evac to waste + .Handoff.select :blocked +2 "valve0"D0 +97 ."PROCESSING"Handoff.select :waste +2 "valve0"D +56 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C\\% +3 "core" %<Gx +47 "syringe1"C]% +3 "core" %<Gx +47 "syringe1"C^% +3 "core" %<Gx +47 "syringe1"C_% +3 "core" %<G +47 "syringe1"C`% +5 "core" %<G"v +46 "syringe1"Ca% +3 "core" %<Gv +46 "syringe1"Cc% +3 "core" %<Gw +48 "syringe1"Cb% +3 "core" %<Gn +47 "syringe1"Cd% +3 "core" %<Go +48 "syringe1"C$% +4 "core" ä%<G$k +45 "syringe1"C&% +3 "core" æ%<Gn +47 "syringe1"C{% +3 "core" %<Gp +47 "syringe1"Cg% +3 "core" %<G +47 "syringe1"CU% +5 "core" %<Gq +45 "syringe1"Cj% +4 "core" %<Gp +46 "syringe1"Ck% +4 "core" %<G؊ +46 "syringe1"Ce% +3 "core" %<G Ԋ +47 "syringe1"Cf% +3 "core" %<G׊ +47 "syringe1"Cl% +4 "core" %<GҊ +46 "syringe1"Cm% +4 "core" %<G֊ +46 "syringe1"Cn% +4 "core" %<GԊ +47 "syringe1"Cq% +3 "core" %<GЊ +46 "syringe1"Cr% +3 "core" %<GΊ +47 "syringe1"Cs% +3 "core" %<G̊ +47 "syringe1"Ci% +3 "core" %<GȊ +47 "syringe1"Cu% +4 "core" %<GŊ +46 "syringe1"Cv% +3 "core" %<G ŋ +47 "syringe1"Co% +3 "core" %<GNj +35 "Can"no<-z9tx t?H +13 "syringe1"Cp% +2 "core" %<G‹ +47 "syringe1"Cw% +3 "core" %<GNj +47 "syringe1"Cx% +3 "core" %<Gċ +47 "syringe1"C% +3 "core" ß%<G +47 "syringe1"Cy% +3 "core" %<G +48 "syringe1"C*% +3 "core" ê%<G! +46 "syringe1"C7% +3 "core" ÷%<G/ +47 "syringe1"C#% +4 "core" ã%<G4 +46 "syringe1"C"% +5 "core" â%<G‹4 +45 "syringe1"C% +4 "core" %<G%‹4 +46 "syringe1"C% +4 "core" Ã%<G$ +46 "syringe1"C% +3 "core" Å%<G  +48 "syringe1"C\ % +3 "core" Ê%<G  +46 "syringe1"C % +3 "core" Ë%<G +47 "syringe1"Cz% +3 "core" %<G9 +47 "syringe1"C:% +3 "core" ú%<G$@ +47 "syringe1"Ct% +3 "core" %<GF +48 "syringe1"C% +4 "core" Î%<GP +45 "syringe1"C% +3 "core" Ð%<GS +47 "syringe1"C% +3 "core" Ò%<G< +47 "syringe1"C% +3 "core" Ô%<G. +47 "syringe1"C% +3 "core" Ö%<G0 +47 "syringe1"C% +3 "core" Ý%<G 2 +47 "syringe1"C% +4 "core" Æ%<G ^ +46 "syringe1"C% +3 "core" È%<G O +47 "syringe1"C% +4 "core" Ü%<Gz +2 ."PROCESSING"Handoff.select :blocked +2 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"D': +83 "core" ě' +2 !"PROCESSING"o:Overpressure: mesg"!Waste port clogged (14.9psi):@timestampu: Time %:bt["H/home/esp/esp2/protocol/3G/function/LyseGo.rb:104:in `evacSWpumpTo'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:381:in `lyse'"D/home/esp/esp2/protocol/3G/function/LyseGo.rb:407:in `lyseHold'"?/home/esp/esp2/protocol/3G/common3Gcfg.rb:19:in `lyseHold'";/home/esp/esp2/utils/cartridge/common.rb:384:in `call'">/home/esp/esp2/utils/cartridge/common.rb:384:in `process'">/home/esp/esp2/utils/auv/cmd.rb:488:in `startProcessing!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" +4116 #"na"Session Begun +1570 #-> showlog +2852 #-> Syringe.psi +3 "syringe1"C% +5 "core" Ú%<G +1237 #"na"-> Handoff.open +3 .Handoff.select :open +5 !o:Valve::Manifold::Error: mesg"3Handoff Valve::Manifold has no selection: open:@timestampu: Time 8:bt["//home/esp/esp2/lib/valve.rb:171:in `fetch'"./home/esp/esp2/lib/valve.rb:176:in `call'"//home/esp/esp2/lib/valve.rb:176:in `fetch'"//home/esp/esp2/lib/valve.rb:201:in `goal='"8/home/esp/esp2/lib/valve.rb:213:in `method_missing'"na:3:in `topLevelBinding'" Syringe.psi +3 "syringe1"CW% +4 "core" %<G +339 "Can"oo-z5t~@Y +247 #"na"-> Handoff.waste +2 .Handoff.select :waste +5 "valve0"D +323 #"na"-> Syringe.psi +3 "syringe1"C% +4 "core" Ø%<G +615 #"na"-> Syringe.psi +2 "syringe1"C% +5 "core" Þ%<G +409 #"na"-> Syringe.psi +3 "syringe1"C% +5 "core" Ï%<G  +4839 #"na"-> exit +51 #Session Ended +5446 "Can"oo-z5tu IAճ +11954 pp-z5tx LB +11955 npE-z1tw DH +11956 qpt-z3tt 4E/ +10190 #"na"Session Begun +680 #-> Handoff +1086 "Can"rp-z;ty FM +37 #"na"-> PV +3 "valve0"D% +4 "core" ę%:7~)Oa 8 +1070 #"na"-> PV.pBottom +4 .Plunge.seek :pBottom +5 "valve0"D'B +85 "core" đ' +5 "valve0"D% +10 "core" ē%B()'X - +572 #"na"-> Handoff.waste +2 .Handoff.select :waste +341 #-> Syringe.psi +3 "syringe1"C % +4 "core" Í%<GÌI +4834 #"na"-> Cart.dry +1695 #-> PhytoSampler.goalVolume +1144 #-> start {fire lysis} +16 !"detached from na"o:Cartridge::None: mesg",No dry microSPR_phyto cartridges remain:@timestampu: Time 0vB:bt["5/home/esp/esp2/utils/auv/cmd.rb:97:in `nextSlot'";/home/esp/esp2/utils/auv/cmd.rb:250:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"na:8:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +776 #"na"-> slot 52 +1341 "Can"mp-z5tx )H:k +2508 #"na"-> lysis=:lyseHold_phyto +1856 #-> slot 52=>[:dry,:lyseHold_phyto] +1891 #-> start {fire lysis} +17 #"detached from na"Selecting Cartridge 52 +6 "carousel0"F'% +6 "core" Ƨ%[- \ +4 "valve0"D(% +3 "core" Ĩ%B(<OR +5 ."detached from na"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E& +7 "core" ŗ& N; OS  +3 ."detached from na"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from na"PinPort.seek :clear +3 "valve1"E)( +80 "core" ũ( +2 ."detached from na"Carousel.in :default +2 "carousel0"F><` F 22 +7 F-% +3 "core" ƭ%[ \ +4 ."detached from na"Carousel.home.seek 22 +4 "carousel0"F.' +15 "core" Ʈ' +2 "carousel0"F % +4 "core" ƌ%)[v 2 +3 "syringe0"B/& +3 "core" ¯&*|  +5 ."Archive"Archive.in :unlocking +3 "syringe1"C0% +5 "syringe0"B? PZ-2 +7 ."Archive"Archive.seek :clear +3 "syringe0"B1(j +5 "core" ð%<G +6 ."Air"Air.in :unlocking +3 "syringe0"B2% +3 "syringe1"C> P]z-2 +9 "core" ²%*ڀ +3 ."Air"Air.seek :clear +5 "syringe1"C ' +5 ."Lysis2"Lysis2.in :unlocking +5 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B'j +388 "core" É' +22 ±( +53 •' +3 "syringe1"C%& +4 "core" å&Xʀ V +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"D% +3 "syringe1"C? P[(2 +7 "core" Ą%B'?Of +3 ."Lysis1"Lysis1.seek :clear +5 "syringe1"C,( +5 ."Plunge"Plunge.in :unlocking +4 "valve0"D> P ޤ(2 +5 ."Plunge"Plunge.seek :clear +3 "valve0"D5'8 +182 "core" ĵ' +16 ì( +3 ."detached from na"Carousel.seek 52 +3 "carousel0"F6'1 +634 "core" ƶ' +4 "carousel0"F8% +3 "core" Ƹ%1/ۿ 2 +3 ."detached from na"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C9& +3 "core" ù&Vˀ +4 ."detached from na"Lysis1.in :fast + "syringe1"C?d P[<2 +6 ."detached from na"Lysis1.seek :locked +4 "syringe1"C!( +250 "core" á( +2 "valve0"D<% +7 "core" ļ%8[:OA +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +5 ."Plunge"Plunge.seek :locked +3 "valve0"D3'Z +7 "syringe1"C % +6 "core" à%ݥ~' +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +2 "syringe1"C='6 +70 "core" ij' +34 ý' +3 ."detached from na"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F>% +3 "core" ƾ%1S۾H  +7 #"detached from na"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."detached from na"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +6 ."detached from na"PuckHeater.configure :default +3 "Cartridge" \ :D H  +6 ."detached from na"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" ?y +5 "cartridge" yVV +4 "Cartridge" @y  +4 "cartridge" y 1&0J59981 4&0{C3 +4 #"detached from na"Mounted lysis Cartridge board#J59981 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"DA% +4 "core" %ZGOO +4 "syringe1"CB& +5 "core" &w +2 "syringe1"CC% +4 "core" %6s V +2 "carousel0"FD% +5 "core" %1ۿs \  +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +4 "valve0"DE% +6 "core" %ZG|OO +3 "syringe1"CF& +5 "core" &u +2 "syringe1"CG% +3 "core" %6t N +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"GH& +7 "core" &l +44 "carousel1"GI& +3 "core" & +47 "carousel1"GJ& +3 "core" & +47 "carousel1"GK& +3 "core" & +47 "carousel1"GL& +3 "core" & +51 "carousel1"GM& +4 "core" & +42 "carousel1"GN& +3 "core" & +47 "carousel1"GO& +12 "core" & +38 "carousel1"GP& +3 "core" &z +47 "carousel1"GQ& +5 "core" &+ +45 "carousel1"GR& +3 "core" & +47 "carousel1"GS& +3 "core" & +47 "carousel1"GT& +4 "core" &0 +46 "carousel1"GV& +3 "core" &F +5 "Can"mp-z5t[ IA + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +7 .PhaseSeparator.in :filling +2 "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold + "carousel1"GX, +5 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +693 "core" ,Jg +7 #"FILTERING"Head pressure reached 3.31psi in 8 seconds +5 ."sealing"Air.in :default +5 "carousel0"FY% +3 "syringe1"C>d P]z<2 +5 "core" %1۾K \  +5 ."sealing"Air.seek :sealed +5 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +3 "syringe1"CZ' +3 ."FILTERING"SamplePump.setPosition! 0ml +10 "sampler0"H7 +6 ."FILTERING"Bypass.open +2 "sampler1"I;1 +150 "core" ɻ1 +73 "ambient0"J[% +3 "core" % } +2 ."FILTERING"Intake.open +3 "sampler0"H\\1 +21 "core" ' +122 1 +2 "ambient0"J]% +6 "core" % +7 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +4 "sampler0"H^% +7 "core" %9 +43 "sampler0"H_% +4 "core" %9 +46 "sampler0"H`% +3 "core" %4 +47 "sampler0"Ha% +4 "core" %p +46 "sampler0"Hc% +3 "core" %r +47 "sampler0"Hb% +3 "core" %k +5 "Can"mp-z5s4J + #"FILTERING"Vacuum tare is 53% of full scale at 26C (drifted 2%) +2 "ambient0"Jd% +8 "core" % +2 "ambient0"J$% +3 "core" ʤ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +7 ."FILTERING"Abs.hold + "ambient0"J&+ +6 "core" ʦ+ +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?B +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G{, +6 "core" , +3 "sampler0"Hg% +6 "core" %b +2 "sampler0"HU% +3 "core" %b +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "12:49:33.00" +1065 "Can"np-z4s3AKE +3464 np-z4s3{Md +4 ."FILTERING"SamplePump.in :start + "sampler0"H>^ }mG#(@A3 22Q +8 ."FILTERING"SamplePump.seek 10ml,1:10 +4 "sampler0"Hj'6 +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }mG#(@A3 22Q +1284 "core" ' +3 "sampler0"Hk% +4 "core" %6q 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +5 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +5 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"He2 +145 "core" 2 +73 ."closeBypass"Bypass.close +4 "sampler1"If/ +146 "core" / +73 "sampler0"Hl% +3 "core" %o +3 "ambient0"Jm% +3 "core" % +3 ."FILTERING"Abs.in :default +3 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold + "ambient0"Jn+ +5 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gq, +6 "core" , +3 "ambient0"Jr% +5 "core" % +3 "sampler0"Hs% +4 "core" %k +3 "sampler0"Hi% +4 "core" % +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }mG#(@3 22Q +8 ."FILTERING"SamplePump.seek 200ml,13:50 +4 "sampler0"Hu'G +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }mG#(@3 22Q +6519 "Can"qp-z2srLS: +11950 lp-zAsqLG\\-ų +7739 "core" ' +2 "sampler0"Hv% +5 "core" %Gӈ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"Io1 +150 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"Hp/ +148 "core" / +2 "carousel0"Fw% +5 "core" %1۽K \  +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :crack +2 "valve0"Dx( +174 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"D(m +208 "core" ğ( +4 ."FILTERING"Toroid.in :default +6 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"Dy( +373 "core" ( +2 "ambient0"J*% +4 "core" ʪ% +5 "Can"lp-zAs=b. + "ambient0"J7% +3 "core" ʷ% +3 "ambient0"J#% +3 "core" ʣ%_ +3 #"FILTERING"Pressurizing cartridge to 14psi above 15.1psia +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Intake.open +2 "sampler0"H"1 +141 "core" Ȣ1 +4 ."FILTERING"Bypass.close +3 "sampler1"I/ +150 "core" / +2 ."FILTERING"Exhaust.close +3 "sampler0"H0 +155 "core" ȃ0 +3 "sampler0"H% +4 "core" ȅ% +2 "ambient0"J\ % +5 "core" ʊ%, +2 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +7 ."FILTERING"Abs.hold + "ambient0"J + +6 "core" ʋ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gz, +7 "core" , +2 "sampler0"H:% +9 "core" Ⱥ% +3 "sampler0"Ht% +5 "core" % +3 ."FILTERING"SamplePump.in :charge +3 "sampler0"H>mGu0\ N3 22Q +6 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +4 "sampler0"H' +1056 "core" Ȏ'  2 +5 "sampler0"H% +3 "core" Ȑ% 2 +3 ."FILTERING"Abs.stop +3 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +6 ."FILTERING"Intake.close +2 "sampler0"H/ +144 "core" Ȓ/ +2 "ambient0"J% +5 "core" ʔ%j +2 #"FILTERING"Pressurized cartridge with 0.471ml to 14.6psi + #Isolated @26.4psia (11.3psi) +6 #Verifying cartridge holds at least 0.65..0.85 of 26.4psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"J% +5 "core" ʖ%p +45 "ambient0"J% +4 "core" ʝ%e +46 "ambient0"J% +4 "core" ʆ%q +47 "ambient0"J% +3 "core" ʈ%j +46 "ambient0"J% +4 "core" ʜ%k +46 "ambient0"J% +4 "core" ʂ%o +46 "ambient0"J% +5 "core" ʚ%d +45 "ambient0"JW% +4 "core" %d +46 "ambient0"J% +4 "core" ʘ%` +47 "ambient0"J% +3 "core" ʞ%b +2 #"FILTERING"Cartridge pressure rose 4% to 27.5psia +3 "carousel0"F% +8 "core" Ə%1۽kJ \  +3 #"FILTERING"Sampling 100.0ml +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +7 ."FILTERING"Exhaust.open +2 "sampler0"H2 +143 "core" ș2 +2 ."FILTERING"Intake.open +3 "sampler0"H1 +143 "core" ȓ1 +4 "Can"lp-zAs@dU +2 "ambient0"J% +3 "core" ʛ%E +11 "ambient0"J % +4 "core" ʍ%E +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +10 ."FILTERING"Abs.hold +4 "ambient0"J'+ +5 "core" ʧ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G(, +6 "core" Ǩ, +2 "ambient0"J% +5 "core" ʗ%A +3 "sampler0"H-% +4 "core" ȭ%  +3 "sampler0"H % +4 "core" Ȍ%  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ mG#(@@N3 22Q +9 ."FILTERING"SamplePump.seek 100ml,6:04 +2 "sampler0"H/'# +56 ."pumpStarted"SamplePump.configure :sample + "sampler0"H#d mG#(@@N3 22Q +227 H0% +3 "core" Ȱ%  2 +3 "ambient0"J2% +3 "core" ʲ%% +3 #"sampling"Cartridge 52 filtering 2/100ml at 0.70ml/s with 9.2psi vacuum +2 "Can"mp-z3svKd +5991 "sampler0"H% +4 "core" ȁ% !vR 2 +2 "ambient0"J4% +5 "core" ʴ%z +2 #"sampling"Cartridge 52 filtering 44/100ml at 0.70ml/s with 9.7psi vacuum 4.7m deep (21.6psia) +5951 "Can"pp-z1suL*m +36 "sampler0"H}% +3 "core" % ^ 2 +2 "ambient0"J% +5 "core" ʇ% +2 #"sampling"Cartridge 52 filtering 85/100ml at 0.69ml/s with 11.1psi vacuum +2141 "core" ȯ' +3 "sampler0"H~% +4 "core" %#Ë 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +5 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +10 ."closeIntake"Intake.close +3 "sampler0"H%/ +6 ."closeExhaust"Exhaust.close +8 ."closeHandoff"Handoff.select :close +55 "sampler0"H0 +6 "carousel0"F|% +4 "valve0"D0 +5 "core" %1۾K \  +8 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"D( +52 "core" ȥ/ +65 Ȅ0 +238 đ( +30 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +11 ."FILTERING"Toroid.seek :clear +4 "valve0"D.( +354 "core" Į( +3 #"FILTERING"Sampled 100.0ml +5 "carousel0"F8% +5 "core" Ƹ%1۾M \  +27 "valve0"D9% +5 "core" Ĺ%ZGOX +2 "syringe1"C<& +3 "core" ü&` +4 "syringe1"C % +4 "core" à% +3 "carousel0"F,% +4 "core" Ƭ%1۽K \  +5 "carousel0"F>% +3 "sampler0"H5% +8 "core" ƾ%1۽JL] \  + ȵ%# +5 ."PROCESSING"Handoff.select :waste +5 "valve0"D +7 ."detached from andia"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E & +11 "core" ʼn&rOR  +2 ."detached from andia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F?% +3 "core" ƿ%1۾_J \  +4 ."detached from andia"Carousel.home.seek 52 +3 "carousel0"F@'1 +9 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CA% +5 "core" %` +2 ' +3 "carousel0"FB% +5 "core" %1-J 2 +3 "syringe1"CC& +3 "core" &k +4 ."detached from andia"Plunge.seek :locked +2 "valve0"DD'Z +7 "core" ' +3 "syringe0"BE& +5 "core" &j؄e f +2 "syringe1"CF% +3 "core" %_ +6 ."Air"Air.in :unlocking +2 "syringe1"CG& +4 C> P]z-2 +7 ."Air"Air.seek :clear +3 "syringe1"CH' +6 "core" &i +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +4 "syringe0"BI% +9 ."Lysis1"Lysis1.seek :clear +3 "syringe1"CJ( +6 CK% +4 "core" %j٘% + %\ g>2 {- +4 "syringe1"CL% +5 "core" % CM1C z-2 +32 "syringe1"CM% +4 "core" % r6 v- +37 ."sourcePumpShutdown"Gate.power :sampler,:OFF +9 "syringe1"CN% +4 "core" %Y) A - +10 ' +14 ( +3 "sampler0"HO% +5 "core" %#`\ +4 ."closeHandoff"Handoff.select :close +3 "valve0"D0 +8 "syringe1"CP% +3 "core" %ݧȀ +46 ."detached from andia"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +3 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785344251.91EDT @EDT1785344252.54 !"PROCESSING"o: BusOff : mesg"Jcore bus was not powered while reading Storage Carousel (SC)'s status:@timestampu: Time :@checkpoint: omitted:bt["1/home/esp/esp2/lib/i2c/sleepy.rb:500:in `<<'"1/home/esp/esp2/lib/i2c/msg.rb:779:in `write'"0/home/esp/esp2/lib/i2c/msg.rb:419:in `send'"2/home/esp/esp2/lib/i2c/servo.rb:559:in `send'"9/home/esp/esp2/lib/i2c/msg.rb:444:in `await1stReply'":/home/esp/esp2/lib/i2c/msg.rb:475:in `awaitOnlyReply'"5/home/esp/esp2/lib/i2c/servo.rb:1146:in `status'"//home/esp/esp2/lib/axis.rb:365:in `status'",/home/esp/esp2/lib/msg.rb:68:in `retry'"//home/esp/esp2/lib/axis.rb:365:in `status'"//home/esp/esp2/lib/slide.rb:59:in `status'"5/home/esp/esp2/lib/slide.rb:181:in `rawPosition'"2/home/esp/esp2/lib/slide.rb:227:in `position'"B/home/esp/esp2/utils/cartridge/carousel.rb:311:in `selected?'"B/home/esp/esp2/utils/cartridge/carousel.rb:318:in `selected!'"?/home/esp/esp2/utils/cartridge/carousel.rb:129:in `update'"?/home/esp/esp2/utils/cartridge/carousel.rb:153:in `state='">/home/esp/esp2/utils/cartridge/common.rb:387:in `process'">/home/esp/esp2/utils/auv/cmd.rb:488:in `startProcessing!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" +8345 "Can"pq-z6tpS +11955 oq-zGtp= +1798 #"na"-> Power +2345 #-> Syringe.psi +5 !o: BusOff : mesg"Gcore bus was not powered while reading Air Syringe (AirS)'s status:@timestampu: Time d:@checkpoint: omitted:bt["1/home/esp/esp2/lib/i2c/sleepy.rb:500:in `<<'"1/home/esp/esp2/lib/i2c/msg.rb:779:in `write'"0/home/esp/esp2/lib/i2c/msg.rb:419:in `send'"2/home/esp/esp2/lib/i2c/servo.rb:559:in `send'"9/home/esp/esp2/lib/i2c/msg.rb:444:in `await1stReply'":/home/esp/esp2/lib/i2c/msg.rb:475:in `awaitOnlyReply'"5/home/esp/esp2/lib/i2c/servo.rb:1146:in `status'"//home/esp/esp2/lib/axis.rb:365:in `status'",/home/esp/esp2/lib/msg.rb:68:in `retry'"//home/esp/esp2/lib/axis.rb:365:in `status'"//home/esp/esp2/lib/slide.rb:59:in `status'"4/home/esp/esp2/utils/common3g.rb:49:in `counts'"-/home/esp/esp2/lib/sensor.rb:21:in `psi'"na:14:in `topLevelBinding'" ESP.configure +6 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +10 #Powering core bus +265 "valve0"DP +3 DT +3 "core"  +7 ."na"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."na"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."na"Toroid.configure :default +2 "valve0"D$ ֜2 +8 ."na"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."na"Abs.configure :default +2 "ambient0"J#: e  +8 ."na"PhaseSeparator.configure :full +3 "carousel1"G$B +8 ."na"Archive.configure :default + "syringe0"B$d PZ<2 +10 ."na"Air.configure :default + "syringe1"C#d P]z<2 +7 ."na"Lysis1.configure :default +2 "syringe1"C$d P[<2 +5 ."na"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +33 ."na"PinPort.configure :default +5 "valve1"E$ B22 +8 #"na"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +309 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !"na"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"na:15:in `topLevelBinding'" Syringe.psi +3 "syringe1"CV% +4 "core" %ݦu +5817 "Can"nr-z3t|\ q[ +11961 orK-z6t|\ ry +11958 qr-z4tz\ t! +11958 qr-z3t}\ uQ +11956 mr-z4ty\ vӳ +6421 #"na"Session Ended +5536 "Can"os-z5tz\ w +11959 osA-z3tz\ x +11959 qsU-z5t|\ &z- +11958 ps~-z3t~\ {?K +11959 ps-z@tz\ "|oi +11960 ns-z5tx\ } +11960 ps-z4t{\ ~ +11960 ss-z exit +6 #Session Ended +9018 "Can"qtu-z2t{\ % ѳ +2884 #"benjamin"Session Begun +1324 #-> Cart.unlock +6 "valve1"EY& +3 "core" &\ OM ,} +4 !"benjamin"o:Slide::Error\ : mesg")Handoff Pin Port (PP) Not yet Homed!:@timestampu: Time ˵: @replyo:I2C::Servo::Status: @pwmi:@deltaPressiMO:@pastThresholdF: @voltagef14.970087383406971: @currenti: @type2F:@outPressi:\ @lostT: @inPressi\ :@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri},:@positioni: @pastRLSF:@axisName"Handoff Pin Port (PP):bt["5/home/esp/esp2/lib/slide.rb:183:in `rawPosition'"//home/esp/esp2/lib/axis.rb:672:in `after?'"C/home/esp/esp2/utils/cartridge/carousel.rb:279:in `disconnect'"B/home/esp/esp2/utils/cartridge/carousel.rb:217:in `disengage'"?/home/esp/esp2/utils/cartridge/carousel.rb:224:in `unlock'"$benjamin:1:in `topLevelBinding'" ESP.ready! +3 "valve0"D;% +7 "core" Ļ%G}ON ' +2 "valve0"D[& +3 "carousel0"F=% +4 "syringe0"B\\& +6 ."Plunge_home"Plunge.home.jog 0 +13 "syringe1"C]% +12 C^& +4 "syringe0"B_% +62 "valve1"E`& +4 "valve0"Da) +19 "core" &OR  + ƽ%O}T % + &؟ @ + %ݫ + &L h %ٕ^  + &OK ,u + ) +10 ."PinPort_home"PinPort.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +4 "valve0"Dc% +4 ."Archive_home"Archive.home.jog 0 +6 ."Toroid_home"Toroid.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +5 "valve1"Eb* +7 "syringe0"Bd) +4 "syringe1"C$) +3 "carousel0"Fg) +7 "syringe0"BU* +7 "valve0"DX* +2 "syringe1"C+* +20 "core" %cGOO  * + ) + ä) + ) * + *\ ؖ-rC I + ë* +3 "syringe0"Be% +11 "syringe1"C&& +6 "valve1"Ek& +5 "valve0"D{& +5 ."Plunge_home"Plunge.in :homing +3 "syringe1"Cf% +5 ."Archive_home"Archive.in :default +8 "valve0"D> (2 +5 "syringe0"B?d PZ<2 +6 "core" %qي + æ&Z[ +2 &OC  + &}O; %ݤ +6 ."Archive_home"Archive.home.jog 0 +9 ."Plunge_home"Plunge.jog 1460 +4 ."Lysis2_home"Lysis2.in :homing +6 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +3 ."PinPort_home"PinPort.in :homing +4 "syringe0"Bl* +3 "valve0"Dm) +2 "syringe0"B> (2 +3 "syringe1"C> -2 +3 "valve0"D?2 +7 "syringe1"C? (2 +3 "valve1"E?2 +11 ."Lysis2_home"Lysis2.jog 1623 +11 ."Lysis1_home"Lysis1.jog 1600 +8 ."Toroid_home"Toroid.jog 1359 +3 ."Air_home"Air.jog 1427 +5 ."PinPort_home"PinPort.jog 1231 +5 "syringe0"Br)W +3 "syringe1"Cs*@ +5 "valve0"Di*O +3 "syringe1"Cj) +2 "valve1"Ev* +20 "core" * +2 "syringe0"Bo& +6 "core" &ؤ>v R +3 ."Archive_home"Archive.in :homing +2 "syringe0"B? -2 +7 ."Archive_home"Archive.jog 1595 +3 "syringe0"Bn*; +10 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +5 "valve0"Dq' +23 "core" ' +4 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +15 "core" ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +6 "syringe0"Bp' +2 "core" ) +2 * +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 ."Air_home"Air.setRawPosition! 0 +3 "syringe1"C82 +4 C7 +4 ."Lysis1_home"Lysis1.seek :home +7 ."Air_home"Air.seek :home +5 "syringe1"Cw( +3 Ch' +19 "core" ' + * + ' + * +4 ."Lysis2_home"Lysis2.in :default +3 ."Air_home"Air.in :default +4 ."PinPort_home"PinPort.setRawPosition! 3150 +4 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe0"B>d P\\k<2 +2 "syringe1"C>d P]z<2 +3 "valve1"E8 N +3 "syringe0"B82 +12 ."PinPort_home"PinPort.seek :home +3 ."Archive_home"Archive.seek :home +5 "valve1"Ex( +4 "syringe0"B( +10 "core" ( +3 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +12 "core" Ÿ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +182 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D*( +6 "core" Ī( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +197 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"E#& +4 "valve0"D7( +5 "core" ţ&Ob  +6 ."benjamin"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."benjamin"PinPort.seek :clear +5 "valve1"E"( +40 "core" Ţ( +289 ķ( +3 ."benjamin"Air.seek :clear +3 "syringe1"C' +6 "core" ' +3 ."benjamin"Lysis1.seek :clear +3 "syringe1"C( +69 "core" Ã( +3 ."benjamin"Lysis2.seek :clear +3 "syringe0"B'j +78 "core" …' +3 ."benjamin"Archive.seek :clear +5 "syringe0"B\ (j +74 "core" Š( +2 ."benjamin"Carousel.in :default + "carousel0"F><` F 22 +7 F:% +3 "core" ƺ%1V_  +3 ."benjamin"Carousel.home.seek 52 +4 "carousel0"Ft'1 +17 "core" ' +2 "valve0"Du% +6 "core" %^Oa !f +4 "valve0"D% +4 "core" Đ%^O` !o +44 "sampler0"H% +5 "core" Ȓ%> +3 "valve0"D& +3 "core" Ĕ&OV . +4 "carousel0"F% +4 "core" Ɩ%1P:I  +3 "ambient0"J% +4 "core" ʝ% +2 "carousel1"G& +5 "core" dž&Px +2 "syringe0"B & +5 "core" ‹&j +2 "syringe1"Cz% +4 "core" %ݰ +3 "syringe1"C& +5 "core" È&t} +2 "syringe0"B% +3 "core" œ%j٨ +4 "valve1"E& +3 "core" ł&OJ x +1150 #"benjamin"-> SC.to 22 +3 .Carousel.seek 22 +5 "carousel0"F' +634 "core" ƚ' +2 "carousel0"FW% +5 "core" %;[ 2 +2062 "Can"rt-z3tu\ [ +11960 ot-z6tu\ \ +11961 st-z3tu\ \ + +11965 pt-}z3tv\ "\ I +2081 #"preston"Session Begun +354 #-> slot +2972 #-> exit +3 #Session Ended +6553 "Can"pt-~z2tz\ e\ g +8463 #"preston"Session Begun +281 #-> progress +2337 #-> Thread["detached from na"].abort +5 !"detached from na"o:Cmd::Error: mesg"Not Processing:bt["=/home/esp/esp2/utils/auv/cmd.rb:529:in `abortProcessing'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'"4/home/esp/esp2/utils/auv/cmd.rb:444:in `finish'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"na:12:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +9 !o:Thread::Aborted : mesg""Aborted by preston":@timestampu: Time v: @cause"detached from na:@checkpoint: omitted:@threadIdixN:bt["0/home/esp/esp2/lib/thread.rb:482:in `abort'"#preston:2:in `topLevelBinding'" Thread[:PROCESSING].abort +4 !"PROCESSING"o:Thread::Aborted : mesg""Aborted by preston": @cause:PROCESSING:@threadIdiN:bt["0/home/esp/esp2/lib/thread.rb:482:in `abort'"#preston:3:in `topLevelBinding'" progress +2836 #-> MainThread.exit +3 "sampler0"H% +3 "core" Ș%P +7 #mEXIT +6 !"preston"o:ThreadError: mesg"killed thread:@timestampu: Time 0 :bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +10 #Session Ended +4 #"benjamin"Session Ended @1785347861.45EDT @1785347873.50EDT = =tMAIN =mMAIN_scheduler @EDT1785347874.68 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +137 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +32 "Can"rt-{z:tz :k\ +318 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +70 #Sea pressure (Abs) tare is 6% of full scale +243 "Can"rt-{z:tc }\ o + rt-{z:tc }\ o + #tPolling Can every 2:00 +41 #Waiting for espclients... +11 "Can"pt-~z3tx\ \ +120 #"preston"Session Begun +1763 #-> Cart.type=:lyseHold_phyto +4 "carousel0"F% +5 "core" Ɓ%Y[  +603 #"preston"-> slot +558 #-> SC +3 "carousel0"F% +4 "core" Ƃ%Y[  +595 #"preston"-> slot +8303 "Can"qt-z1tw\ \ +2146 #"preston"-> PhytoSampler.goalVolume=50; PhytoSampler.primeVolume=25 +4382 #-> SC +3 "carousel0"F% +4 "core" ƃ%Z[  +2298 #"preston"-> slot 52 +1422 #-> slot 52=>:dry +1179 #-> lysis +523 "Can"st-}z4tt\ \ Ւ +2155 #"preston"-> lysis=:lyseHold_phyto +3722 #-> start {fire lysis} +36 ."detached from preston"Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +6 .Gate.power :analytic1,:ON,limit to 4000mA +53 "valve0"DP +3 D +3 "core" Ą +6 "valve0"D% +4 "core" ą%^OJ !q +3 ."detached from preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 "sampler0"H% +3 "core" Ȇ%B +2 ."detached from preston"SamplePump.configure :default +3 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" ć&OL  +3 ."detached from preston"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from preston"Toroid.hold +3 "valve0"D, +5 "core" Ĉ, +3 ."detached from preston"Toroid.configure :default +2 "valve0"D$ ֜2 +8 "carousel0"F % +4 "core" Ɖ%Z[  +2 ."detached from preston"Carousel.configure :default +3 "carousel0"F#<` F 22 +6 ."detached from preston"Carousel.hold +3 "carousel0"F\ + +6 "core" Ɗ+ +2 ."detached from preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 "ambient0"J % +3 "core" ʋ% +3 ."detached from preston"Abs.configure :default +2 "ambient0"J#  +10 "carousel1"G & +30 "core" nj&Z +3 ."detached from preston"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B & +3 "core" &j +3 ."detached from preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +11 ."detached from preston"Archive.hold +2 "syringe0"B, +6 "core" Ž, +3 ."detached from preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 "syringe1"C% +3 "core" Ï%ݸ& +2 ."detached from preston"Air.configure :default +3 "syringe1"C#d P]z<2 +7 ."detached from preston"Air.hold +2 "syringe1"C+ +5 "core" Ð+ +3 ."detached from preston"Air.configure :default +2 "syringe1"C#d P]z<2 +6 C& +3 "core" Ñ&rh +3 ."detached from preston"Lysis1.configure :default +3 "syringe1"C$d P[<2 +6 ."detached from preston"Lysis1.hold +2 "syringe1"C, +5 "core" Ò, +3 ."detached from preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B% +3 "core" “%j٧l +3 ."detached from preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from preston"Lysis2.hold + "syringe0"B+ +5 "core" ”+ +3 ."detached from preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +35 "valve1"E& +3 "core" ŕ&OK x +3 ."detached from preston"PinPort.configure :default +2 "valve1"E$ B22 +6 ."detached from preston"PinPort.hold +3 "valve1"E, +5 "core" Ŗ, +3 ."detached from preston"PinPort.configure :default + "valve1"E$ B22 +7 #"detached from preston"All elf controllers running firmware version 4.94 +110 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +4 !"detached from preston"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'";/home/esp/esp2/utils/auv/cmd.rb:255:in `loadCartridge'",/home/esp/esp2/lib/msg.rb:68:in `retry'";/home/esp/esp2/utils/auv/cmd.rb:253:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"$preston:11:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +7 "valve0"D% +4 "core" ė%^OK  +2 "valve0"D& +7 "carousel0"F% +6 "syringe0"B& +3 "syringe1"C% +8 C& +4 "syringe0"B% +6 "valve1"E& +10 "core" Ę&OF  + ƙ%Z[/  š&j + Û%ݯ + Ü&vH + %j٤ + Ş&O@  +7 ."clearToroid"Toroid.seek :clear +5 "valve1"E& +5 "valve0"D ( +6 "core" ş&OF  + Ġ( +4 ."detached from preston"Air.seek :clear +2 "syringe1"C!' +12 "core" á' +2 ."detached from preston"Lysis1.seek :clear +3 "syringe1"C"( +5 "core" â( +4 ."detached from preston"Lysis2.seek :clear +3 "syringe0"B#'j +6 "core" £' +3 ."detached from preston"Archive.seek :clear +2 "syringe0"B$(j +7 "core" ¤( +2 ."detached from preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 F%% +2 "core" ƥ%Z["  +4 ."detached from preston"Carousel.home.seek 22 +41 "carousel0"F&' +17 "core" Ʀ' +2 "valve0"D'% +4 "core" ħ%^OR  +13 #"detached from preston"Selecting Cartridge 52 +2 "valve0"D(% +8 "core" Ĩ%^OA +2 "syringe1"C)& +5 "core" é&u +2 "syringe1"C*% +3 "core" ê%ݮr +4 "valve1"E+& +4 "core" ū&OE z +4 ."detached from preston"Carousel.seek 52 +2 "carousel0"F,'1 +635 "core" Ƭ' +4 "carousel0"F-% +3 "core" ƭ%1- 2 +3 ."detached from preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C.& +3 "core" î&l +4 ."detached from preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from preston"Lysis1.seek :locked +3 "syringe1"C/( +249 "core" ï( +4 "valve0"D0% +5 "core" İ%^)Ov  +4 ."Plunge"Plunge.seek :locked +2 "valve0"D1'Z +8 "syringe1"C2% +6 "core" ò%ݴ( +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +8 ."Air"Air.seek :locked +3 "syringe1"C3'6 +78 "core" ı' +26 ó' +2 ."detached from preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F4% +3 "core" ƴ%1Uۿ  +6 #"detached from preston"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."detached from preston"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from preston"PuckHeater.configure :default +2 "Cartridge" \ :D H  +6 ."detached from preston"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" 5t +5 "cartridge" t +2 "Cartridge" 6y +5 "cartridge" yVV +3 "Cartridge" 7y  +5 "cartridge" y 1&0J59981 4&0{C3 +3 #"detached from preston"Mounted lysis Cartridge board#J59981 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D8% +9 "core" ĸ%ZGqOO +4 "syringe1"C9& +5 "core" ù& +3 "syringe1"C:% +3 "core" ú%6 < +3 "carousel0"F;% +4 "core" ƻ%1ۿ \  +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +4 #Cartridge already filtered 100.0ml +6 "valve0"D<% +5 "core" ļ%ZGuOP +3 "syringe1"C=& +4 "core" ý& +3 "syringe1"C>% +4 "core" þ%6 6 +3 "carousel0"F?% +3 "core" ƿ%1ۿi \  +4 !"detached from preston"o:Cartridge::Error: mesg"$Cannot process dry cartridge 52:@timestampu: Time ' :bt["?/home/esp/esp2/utils/auv/cmd.rb:657:in `verifyCartStateIs'">/home/esp/esp2/utils/auv/cmd.rb:470:in `startProcessing!'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"$preston:11:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + #Waiting to Resume from Checkpoint "Selecting Cartridge 52" +3720 "Can"rt-z2ti\ +1576 #"preston"-> Sampler.reset +3447 #-> Thread["detached from preston"].abort +3 !"detached from preston"o:Thread::Aborted : mesg""Aborted by preston": @cause"detached from preston:@threadIdih;L:bt["0/home/esp/esp2/lib/thread.rb:482:in `abort'"$preston:13:in `topLevelBinding'" start {fire lysis} +17 #"detached from preston#2"Selecting Cartridge 52 +6 "carousel0"F@% +8 "core" %1ۿ \  +3 "valve0"DA% +5 "core" %ZGmO? +2 "syringe1"CB& +4 "core" & +2 "syringe1"CC% +4 "core" %6 A +3 "carousel0"FD% +3 "core" %1ۿ \  +4 "valve0"DE% +3 "core" %ZG}O? +3 "syringe1"CF& +4 "core" &" +3 "syringe1"CG% +5 "core" %6 ; +2 "carousel0"FH% +4 "core" %1۾ \  +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +4 #Cartridge already filtered 100.0ml +6 "valve0"DI% +5 "core" %ZGtO= +3 "syringe1"CJ& +4 "core" &% +3 "syringe1"CK% +4 "core" %67 C +3 "carousel0"FL% +3 "core" %1ۿ \  +3 !"detached from preston#2"o:Cartridge::Error: mesg"$Cannot process dry cartridge 52:@timestampu: Time '~:bt["?/home/esp/esp2/utils/auv/cmd.rb:657:in `verifyCartStateIs'">/home/esp/esp2/utils/auv/cmd.rb:470:in `startProcessing!'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"$preston:14:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Selecting Cartridge 52" +944 #"preston"-> Cart.unlock +6 .Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +5 "valve1"EM& +4 "core" &OX  +3 ."preston"Carousel.in :default + "carousel0"F><` F 22 +6 FN% +3 "core" %1ۿ \  +5 ."preston"Carousel.home.seek 52 +3 "carousel0"FO'1 +17 "core" ' +2 "carousel0"FP% +4 "core" %1, 2 +3 "syringe0"BQ& +5 "core" &j +2 "syringe1"CR% +5 "core" %6 ; +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +4 "syringe0"BS% +10 ."Air"Air.seek :clear +3 "syringe1"CT' +6 "core" %j٭f +89 ' +2 "syringe1"CU& +5 "core" & ڀ +4 ."Lysis1"Lysis1.in :unlocking +3 "valve0"DV% +4 "syringe1"C? P[(2 +8 ."Lysis1"Lysis1.seek :clear +3 "syringe1"CW( +4 "core" %ZGjOW +6 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +2 "valve0"DX'8 +96 "core" ' +12 ( +2639 #"preston"-> slot 52=>nil +1446 "Can"rt-z/tz\ M  +1530 #"preston"-> slot 52=>:lyseHold_phyto +632 #-> start {fire lysis} +19 #"detached from preston#3"Selecting Cartridge 52 +5 "valve0"DY% +6 "core" %8[O\\ +5 "syringe1"CZ& +3 "core" &o +3 "syringe1"C[% +5 "core" %ݦ +3 "valve1"E\\& +47 "core" &O^ u +3 ."detached from preston#3"Carousel.seek 52 +3 "carousel0"F]'1 +17 "core" ' +4 "carousel0"F^% +3 "core" %1P  +3 ."detached from preston#3"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C_& +3 "core" &l +4 ."detached from preston#3"Lysis1.in :fast + "syringe1"C?d P[<2 +7 ."detached from preston#3"Lysis1.seek :locked +3 "syringe1"C`( +250 "core" ( +3 "valve0"Da% +8 "core" %8[Oc +2 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +5 "syringe1"Cb% +5 ."Plunge"Plunge.seek :locked +8 "valve0"Dc'Z +3 "core" %ݯ +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"Cd'6 +77 "core" ' +27 ' +3 ."detached from preston#3"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 Fe% +3 "core" %1U  +7 #"detached from preston#3"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from preston#3"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +8 ."detached from preston#3"PuckHeater.configure :default +5 "Cartridge" \ :D H  +4 ."detached from preston#3"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" fy +5 "cartridge" yVV +6 "Cartridge" gy  +5 "cartridge" y 1&0J59981 4&0{C3 +3 #"detached from preston#3"Mounted lysis Cartridge board#J59981 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"Dh% +6 "core" %ZGaOJ +3 "syringe1"Ci& +4 "core" & +2 "syringe1"Cj% +5 "core" %6  G +2 "carousel0"Fk% +4 "core" %1 \ +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +4 "valve0"Dl% +7 "core" %ZG\\OI +2 "syringe1"Cm& +4 "core" & +2 "syringe1"Cn% +5 "core" %6 S +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"Go& +8 "core" & +42 "carousel1"Gp& +4 "core" & +47 "carousel1"Gq& +3 "core" &x +46 "carousel1"Gr& +4 "core" & +47 "carousel1"Gs& +2 "core" & +48 "carousel1"Gt& +3 "core" & +47 "carousel1"Gu& +2 "core" &U +48 "carousel1"Gv& +3 "core" &{ +47 "carousel1"Gw& +3 "core" &. +47 "carousel1"Gx& +5 "core" & +45 "carousel1"Gy& +3 "core" & +47 "carousel1"Gz& +3 "core" & +47 "carousel1"G{& +3 "core" & +47 "carousel1"G|& +3 "core" & +5 "Can"rt-z/tk  +2 #"FILTERING"Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +6 .PhaseSeparator.in :filling + "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G}, +4 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +699 "core" ,N +7 #"FILTERING"Head pressure reached 3.51psi in 8 seconds +4 ."sealing"Air.in :default +4 "carousel0"F~% +3 "syringe1"C>d P]z<2 +6 "core" %1N \ +5 #"FILTERING"Priming sample loop w/25ml, bypass w/10ml +3 ."sealing"Air.seek :sealed +6 ."FILTERING"SamplePump.setPosition! 0ml +5 "syringe1"C' +3 "sampler0"H7 +7 ."FILTERING"Exhaust.close +3 "sampler0"H0 +7 "core" ȁ0 +3 ."FILTERING"Bypass.open +3 "sampler1"I1 +151 "core" ɂ1 +73 "ambient0"J% +3 "core" ʃ%k  +3 ."FILTERING"Intake.open +2 "sampler0"H1 +15 "core" ' +131 Ȅ1 +2 "ambient0"J% +6 "core" ʅ%  +3 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +3 "sampler0"H% +7 "core" Ȇ%1+ +43 "sampler0"H% +5 "core" ȇ%-" +45 "sampler0"H % +3 "core" ȉ% +47 "sampler0"H % +4 "core" ȋ% +46 "sampler0"H % +3 "core" Ȍ%| +47 "sampler0"H % +3 "core" ȍ%z +5 "Can"rt-z/s1  +2 #"FILTERING"Vacuum tare is 54% of full scale at 26C (drifted 2%) +2 "ambient0"J% +8 "core" ʏ% +4 "ambient0"J% +5 "core" ʑ%s +2 ."FILTERING"Abs.in :default + "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +3 "ambient0"J+ +48 "core" ʓ+ +3 ."FILTERING"PhaseSeparator.in :full + "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G, +5 "core" Ǖ, +3 "sampler0"H% +5 "core" ȗ%s +3 "sampler0"H% +3 "core" ȝ%p4 +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "14:07:40.08" +4469 "Can"rt-z/s1C *9 +13 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(A@3 22Q +7 ."FILTERING"SamplePump.seek 10ml,1:10 +8 "sampler0"H'6 +51 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }m#(A@3 22Q +1290 "core" Ȝ' +2 "sampler0"H% +4 "core" ț%6i 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +5 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +6 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +6 ."FILTERING"Exhaust.open +3 "sampler0"H2 +146 "core" Ț2 +76 ."closeBypass"Bypass.close +4 "sampler1"I/ +148 "core" ə/ +73 "sampler0"H% +3 "core" Ș% +3 "ambient0"J% +4 "core" ʞ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +4 "ambient0"J+ +5 "core" ʟ+ +3 ."FILTERING"PhaseSeparator.hold + "carousel1"G%, +8 "core" ǥ, +2 "ambient0"J'% +4 "core" ʧ% +3 "sampler0"H(% +3 "core" Ȩ% +4 "sampler0"H)% +5 "core" ȩ%S +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(A@3 22Q +8 ."FILTERING"SamplePump.seek 25ml,2:10 +2 "sampler0"H*' +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(A@3 22Q +139 "Can"qt-z8sIf / +3114 "core" Ȫ' +2 "sampler0"H+% +4 "core" ȫ% 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +5 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +9 ."FILTERING"Bypass.open +2 "sampler1"I1 +153 "core" Ɉ1 +3 ."FILTERING"Intake.close +2 "sampler0"H/ +148 "core" Ȑ/ +2 "carousel0"F% +6 "core" ƒ%1JG \ +5 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +2 "valve0"D( +171 "core" Ĕ( +77 ."FILTERING"Toroid.seek :cartridge +2 "valve0"D(m +208 "core" Ď( +5 ."FILTERING"Toroid.in :default + "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :clear +3 "valve0"D\ ( +371 "core" Ċ( +2 "ambient0"J&% +5 "core" ʦ%y +4 "Can"qt-z8s:yw :l +2 "ambient0"J-% +3 "core" ʭ%\ +2 "ambient0"J.% +4 "core" ʮ% +4 #"FILTERING"Pressurizing cartridge to 14psi above 15.0psia +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Intake.open +3 "sampler0"H01 +146 "core" Ȱ1 +3 ."FILTERING"Bypass.close +2 "sampler1"I2/ +151 "core" ɲ/ +3 ."FILTERING"Exhaust.close +2 "sampler0"H"0 +152 "core" Ȣ0 +2 "sampler0"H4% +6 "core" ȴ% +2 "ambient0"J!% +4 "core" ʡ%q% +3 ."FILTERING"Abs.in :default + "ambient0"J>9 e  +7 ."FILTERING"Abs.hold +2 "ambient0"J5+ +5 "core" ʵ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G6, +6 "core" Ƕ, +3 "sampler0"H7% +5 "core" ȷ% +2 "sampler0"H8% +3 "core" ȸ% +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>mu0r3 22Q +7 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +4 "sampler0"H9' +861 "core" ȹ'O. 2 +5 "sampler0"H:% +3 "core" Ⱥ%})- 2 +3 ."FILTERING"Abs.stop +3 "ambient0"J5 +5 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Intake.close +2 "sampler0"H;/ +148 "core" Ȼ/ +2 "ambient0"J<% +5 "core" ʼ%. +2 #"FILTERING"Pressurized cartridge with 0.312ml to 18.9psi +2 #Isolated @22.1psia (7.1psi) +5 #Verifying cartridge holds at least 0.65..0.85 of 22.1psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"J=% +7 "core" ʽ%' +43 "ambient0"J>% +3 "core" ʾ% +47 "ambient0"J?% +3 "core" ʿ% +47 "ambient0"J@% +49 "core" %% +3 "ambient0"JA% +14 "core" %% +34 "ambient0"JB% +3 "core" %) +47 "ambient0"JC% +4 "core" %& +46 "ambient0"JD% +3 "core" %# +47 "ambient0"JE% +3 "core" % +47 "ambient0"JF% +3 "core" % +3 #"FILTERING"Cartridge pressure fell 1% to 21.9psia +2 "carousel0"FG% +7 "core" %1K \ +3 #"FILTERING"Sampling 50.0ml +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Exhaust.open +3 "sampler0"HH2 +144 "core" 2 +2 ."FILTERING"Intake.open +3 "sampler0"HI1 +146 "core" 1 +4 "Can"qt-z8s= @w +2 "ambient0"JJ% +5 "core" % +4 "ambient0"JK% +5 "core" %- +2 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +7 ."FILTERING"Abs.hold +3 "ambient0"JL+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GM, +7 "core" , +2 "ambient0"JN% +4 "core" % +4 "sampler0"HP% +4 "core" %  +2 "sampler0"HQ% +5 "core" %  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ m#(@@3 22Q +8 ."FILTERING"SamplePump.seek 50ml,3:17 +2 "sampler0"HR' +57 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d m#(@@3 22Q +226 HS% +3 "core" %\ " z 2 +3 "ambient0"JU% +4 "core" %N +2 #"sampling"Cartridge 52 filtering 2/50ml at 0.71ml/s with 13.5psi vacuum +4842 "Can"otv-z6sG M +1151 "sampler0"HV% +3 "core" %h -! 2 +3 "ambient0"J,% +3 "core" ʬ%u~ +3 #"sampling"Cartridge 52 filtering 45/50ml at 0.72ml/s with 15.3psi vacuum +724 "core" ' +2 "sampler0"H3% +5 "core" ȳ% 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +9 ."closeIntake"Intake.close +4 "sampler0"H// +7 ."closeExhaust"Exhaust.close +4 "carousel0"F1% +7 "sampler0"HY0 +10 "core" Ʊ%1OG \ +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :bypass +3 "valve0"DZ( +107 "core" ȯ/ +21 0 +226 ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :clear +6 "valve0"D[( +353 "core" ( +3 #"FILTERING"Sampled 50.0ml +4 "carousel0"F\\% +6 "core" %1O \ +8 "valve0"D^% +3 "core" %ZG}OH +3 "syringe1"C_& +3 "core" & +4 "syringe1"CO% +5 "core" % +6 "carousel0"Fa% +4 "core" %1%M; \ +3 "carousel0"Fb% +6 "core" %1N \ +3 ."PROCESSING"Handoff.select :waste +3 "valve0"D +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"CW% +6 "core" % +45 "syringe1"Ce% +3 "core" %܀ +47 "syringe1"CX% +3 "core" % +47 "syringe1"CT% +4 "core" % +46 "syringe1"Cf% +4 "core" % +2 ."sourcePumpShutdown"Gate.power :sampler,:OFF +44 "syringe1"Cg% +3 "core" % +47 "syringe1"Ch% +3 "core" % +47 "syringe1"Ci% +3 "core" % +48 "syringe1"Cj% +3 "core" % +47 "syringe1"Ck% +4 "core" % +46 "syringe1"Cl% +3 "core" % +47 "syringe1"Cm% +3 "core" % +47 "syringe1"Cn% +3 "core" % +47 "syringe1"Co% +3 "core" % +5 "Can"otv-z6t]  VC +2 #"PROCESSING"Syringe tare is 50% of full scale at 26C (drifted -1%) +3 .PinPort.in :locking +4 "valve1"E? B22 +5 ."PROCESSING"PinPort.seek :locked +5 "valve1"Ep( N +78 "core" ( +3 ."posArchive"Archive.in :fast +6 ."posLyS2"Lysis2.in :fast +6 "syringe0"B?d PZ<2 +3 ."PROCESSING"Lysis1.seek 1250uL +7 "syringe0"B>d P\\k<2 +3 ."posArchive"Archive.seek :empty +6 "syringe1"Cq( +6 ."posLyS2"Lysis2.seek :empty +8 "syringe0"Br( +4 Bs' +241 "core" ( +588 ( +53 ' +2 "carousel0"Ft% +4 "core" %1 \ +7 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +3 .PinPort.seek :locked +5 "valve1"Eu( N +5 "core" ( +3 ."PROCESSING"Handoff.select :waste +9 .Plunge.seek :puckBottom +3 "valve0"Dv'B +128 "core" ' +2 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"Cw% +7 "core" % +43 "syringe1"Cx% +3 "core" % +47 "syringe1"Cy% +3 "core" % +47 "syringe1"Cz% +3 "core" % +47 "syringe1"C{% +3 "core" % +47 "syringe1"C|% +3 "core" % +47 "syringe1"C~% +3 "core" % +47 "syringe1"C% +4 "core" Á% +46 "syringe1"C% +4 "core" Â% +46 "syringe1"C% +4 "core" Ã% +46 "syringe1"Cd% +4 "core" % +46 "syringe1"C% +3 "core" Ä% +47 "syringe1"C% +3 "core" Å% +47 "syringe1"C% +3 "core" Æ% +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi +3 #Air stroke #1 of 1 +4 "syringe1"C% +9 "core" Ç% +3 ."PROCESSING"Air.in :pressurize + "syringe1"C>@N  P]z<2 +7 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C ' +515 "core" É' +2 "syringe1"C % +5 "core" Ë%SƁp < +2 #"PROCESSING"0.9psi after pressurize +2 .Air.in :compress +3 "syringe1"C>N  P]zP2 +8 ."PROCESSING"Air.seek :evac +2 "syringe1"C '| +96 "core" Ì'pO@ vP +4 "syringe1"C % +5 "core" Í%O4 P +2 #"PROCESSING"Reached 4.3 of 4.0psi after pumping 1457uL (0.82 strokes) +2 .Air.in :default +4 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.hold +3 "syringe1"C+ +5 "core" Ï+ +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C% +7 "core" Ñ%O: +43 "syringe1"C% +3 "core" ×%O85 +48 "syringe1"C% +5 "core" Ý%O6 +44 "syringe1"C}% +4 "core" %O:. +46 "syringe1"C% +3 "core" Û%O8h +47 "syringe1"C% +3 "core" Ú%O> +48 "syringe1"C% +5 "core" Ó%O: +44 "syringe1"C% +4 "core" Õ%OA +46 "syringe1"C% +3 "core" Ù%O5 +47 "syringe1"C% +3 "core" Ø%OF- +49 "syringe1"C% +4 "core" Þ%O;? +44 "syringe1"C'% +3 "core" ç%O5{[ +47 "syringe1"C(% +3 "core" è%O9Շo +48 "syringe1"C)% +3 "core" é%O:v +49 "syringe1"C% +3 "core" Ü%O3{ +44 "syringe1"C+% +4 "core" ë%O?{ +46 "syringe1"C% +4 "core" È%O8z +46 "syringe1"C% +5 "core" ß%O?| +47 "syringe1"C%% +4 "core" å%O8x +44 "syringe1"C% +3 "core" Ð%O6x +47 "syringe1"C% +3 "core" Ò%O;} +47 "syringe1"C % +3 "core" à%O9y +50 "syringe1"C% +3 "core" Ô%O7}\ +44 "syringe1"C% +4 "core" Î%O5t- +46 "syringe1"C&% +4 "core" æ%O9x3 +46 "syringe1"C-% +3 "core" í%O=x= +49 "syringe1"C.% +4 "core" î%O:r_ +44 "syringe1"C0% +5 "core" ð%O3sm +45 "syringe1"C2% +3 "core" ò%O<u +47 "syringe1"C"% +3 "core" â%O5t +49 "syringe1"C4% +4 "core" ô%O8\ +44 "syringe1"C!% +4 "core" á%O6t +46 "syringe1"C7% +4 "core" ÷%O0t +46 "syringe1"C8% +4 "core" ø%O9p +47 "syringe1"C*% +4 "core" ê%O<s +45 "syringe1"C:% +3 "core" ú%O9u +48 "syringe1"C;% +3 "core" û%O: +46 "syringe1"C<% +4 "core" ü%O< +48 "syringe1"C=% +4 "core" ý%O7 +45 "syringe1"C>% +3 "core" þ%O* +46 "syringe1"C?% +4 "core" ÿ%O; +46 "syringe1"C5% +4 "core" õ%O9 +2 #"PROCESSING"Stabilized at 11.1psi after evac to waste + .Handoff.select :blocked +5 "valve0"D0 +99 ."PROCESSING"Handoff.select :waste +3 "valve0"D +103 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C6% +5 "core" ö%O> +45 "syringe1"C@% +4 "core" %O: +46 "syringe1"CA% +5 "core" %O4 +45 "syringe1"CB% +4 "core" %O9 +47 "syringe1"CC% +3 "core" %O0 +46 "syringe1"CD% +3 "core" %O4 +47 "syringe1"CE% +3 "core" %O; +47 "syringe1"CF% +4 "core" %O4 +47 "syringe1"CG% +3 "core" %O7 +47 "syringe1"CH% +4 "core" %O? +46 "syringe1"CI% +3 "core" %O5 +47 "syringe1"CJ% +3 "core" %O< +47 "syringe1"CK% +4 "core" %O7\ +46 "syringe1"CN% +3 "core" %O8 +47 "syringe1"CP% +3 "core" %O1 +47 "syringe1"CQ% +3 "core" %O: +47 "syringe1"CS% +3 "core" %O: +47 "syringe1"CU% +4 "core" %O7 +3 ."PROCESSING"Handoff.select :blocked +2 "valve0"D0 +51 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"D9': +86 "core" Ĺ' +3 !"PROCESSING"o:Overpressure: mesg"!Waste port clogged (11.3psi):@timestampu: Time _.:bt["H/home/esp/esp2/protocol/3G/function/LyseGo.rb:104:in `evacSWpumpTo'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:381:in `lyse'"D/home/esp/esp2/protocol/3G/function/LyseGo.rb:407:in `lyseHold'"?/home/esp/esp2/protocol/3G/common3Gcfg.rb:19:in `lyseHold'";/home/esp/esp2/utils/cartridge/common.rb:384:in `call'">/home/esp/esp2/utils/cartridge/common.rb:384:in `process'">/home/esp/esp2/utils/auv/cmd.rb:488:in `startProcessing!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" +2659 "Can"qtu-z1tu k +3549 #"preston"-> Syringe.psi +3 "syringe1"CV% +5 "core" %O1 +556 #"preston"-> Handoff.waste +5 .Handoff.select :waste +2 "valve0"D +390 #"preston"-> Syringe.psi +4 "syringe1"C,% +3 "core" ì%O4{ +352 #"preston"-> Handoff.waste +3 .Handoff.select :waste +278 #-> Syringe.psi +2 "syringe1"C3% +5 "core" ó%O6{ +409 #"preston"-> Handoff.waste +6 .Handoff.select :waste +377 #-> PV +3 "valve0"D1% +4 "core" ı%:7yOU = +578 #"preston"-> PV.pBottome +5 !o:NoMethodError\ : mesgu:NameError::message1undefined method `pBottome' for Plunge:Slide:@timestampu: Time i4: name: pBottome: args[:bt["8/home/esp/esp2/lib/slide.rb:459:in `method_missing'"$preston:26:in `topLevelBinding'" PV.pBottom +3 .Plunge.seek :pBottom +5 "valve0"D/'B +85 "core" į' +2 "valve0"DY% +3 "core" %B(+uX - +654 #"preston"-> PV.pTop +5 .Plunge.seek :pTop +3 "valve0"DL': +84 "core" ' +2 "valve0"DM% +4 "core" %:7MX -5 +381 #"preston"-> PV.pBottom +4 .Plunge.seek :pBottom +3 "valve0"D\ 'B +84 "core" Ċ' +3 "valve0"DZ% +5 "core" %B(0:Y - +1029 #"preston"-> Handoff.waste +3 .Handoff.select :waste +2816 "Can"rt-z3tv = +1439 #"preston"-> Cart.unlock +6 .Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E\\& +4 "core" & N;OL +3 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."preston"PinPort.seek :clear +3 "valve1"E^( +80 "core" ( +2 ."preston"Carousel.in :default + "carousel0"F><` F 22 +7 F_% +3 "core" %1a \ +3 ."preston"Carousel.home.seek 52 +3 "carousel0"FO'1 +17 "core" ' +2 "carousel0"Fa% +4 "core" %1O  +3 "syringe0"Bb& +3 "core" &) +5 ."Archive"Archive.in :unlocking +4 "syringe1"CW% +6 "syringe0"B? PZ-2 +10 ."Archive"Archive.seek :clear +2 "syringe0"Be(j +6 "core" %O5 +5 ."Air"Air.in :unlocking +5 "syringe0"BX% +2 "syringe1"C> P]z-2 +7 ."Air"Air.seek :clear +4 "syringe1"CT' +6 "core" %*Ҁ +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bf'j +365 "core" ' +47 ( +50 ' +3 "syringe1"Cg& +4 "core" &[ǀ 3 +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"Dh% +6 "syringe1"C? P[(2 +4 "core" %B(%&OX +4 ."Lysis1"Lysis1.seek :clear +5 "syringe1"Ci( +6 ."Plunge"Plunge.in :unlocking +4 "valve0"D> P ޤ(2 +5 ."Plunge"Plunge.seek :clear +3 "valve0"Dj'8 +181 "core" ' +18 ( +1146 #"preston"-> MainThread.exit +4 "sampler0"Hk% +3 "core" % +5 ."closeHandoff"Handoff.select :close +9 "valve0"D0 +50 #mEXIT +4 #"preston"Session Ended @1785348876.43EDT @1785350562.77EDT = =tMAIN =mMAIN_scheduler @EDT1785350564.15 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +151 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +225 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +389 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +73 #Sea pressure (Abs) tare is 6% of full scale +270 "Can"oc+fz8tyx +35 oc+fz8tyx #tPolling Can every 2:00 +9 #Waiting for espclients... +43 "Can"nc +mz:t6z +62 #"preston"Session Begun +8395 #-> slot +665 #-> slot 22 +1856 #-> slot 22=>[:lyseHold_phyto,:dry] +336 #-> slot 52 +655 "Can"o`@+z4t< +241 #"preston"-> slot 52=>nil +923 #-> slot 52=>[:lyseHold_phyto,:dry] +426 #-> Cart.dry +1851 #-> lysis=lyseHold_phyto +772 #-> lysis +5675 #"han"Session Begun +2081 "Can"l^o+zJtZ +3261 #"preston"-> PhytoSampler.goalVolume=100 +926 #-> PhytoSampler.primeVolume=25 +496 #"benji"Session Begun +477 #"han"-> start {fire lysis} +21 !"detached from han"o:Cartridge::None: mesg",No dry microSPR_phyto cartridges remain:@timestampu: Time ꧾ:bt["5/home/esp/esp2/utils/auv/cmd.rb:97:in `nextSlot'";/home/esp/esp2/utils/auv/cmd.rb:250:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"han:1:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +4889 #"han"-> lysis=:lyseHold_phyto +621 #-> lysis +1230 #-> start {fire lysis} +25 ."detached from han"Gate.power :sampler,:OFF,limit to 3500mA +8 .Gate.power :skimmer,:OFF,limit to 3500mA +8 .Gate.power :main,:ON,limit to 5000mA +6 "Can"i],z6tx +4 ."detached from han"Gate.power :analytic1,:ON,limit to 4000mA +25 #Powering core bus +265 "valve0"DP +3 D +3 "core" ā +8 ."detached from han"Plunge.configure :default +2 "valve0"D# P ޤ-2 +13 ."detached from han"SamplePump.configure :default +2 "sampler0"H#^ #(22 +11 ."detached from han"Toroid.configure :default +2 "valve0"D$ ֜2 +12 ."detached from han"Carousel.configure :default +2 "carousel0"F#<` F 22 +12 ."detached from han"Abs.configure :default +3 "ambient0"J#  +11 ."detached from han"PhaseSeparator.configure :default +3 "carousel1"G$ +36 ."detached from han"Archive.configure :default +2 "syringe0"B$d PZ<2 +13 ."detached from han"Air.configure :default +3 "syringe1"C#d P]z<2 +8 ."detached from han"Lysis1.configure :default +2 "syringe1"C$d P[<2 +9 ."detached from han"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +11 ."detached from han"PinPort.configure :default +2 "valve1"E$ B22 +14 #"detached from han"All elf controllers running firmware version 4.94 +8 .Gate.power :motor,:ON,limit to 5000mA +251 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +6 !"detached from han"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'";/home/esp/esp2/utils/auv/cmd.rb:255:in `loadCartridge'",/home/esp/esp2/lib/msg.rb:68:in `retry'";/home/esp/esp2/utils/auv/cmd.rb:253:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"han:4:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +10 "valve0"D% +6 "core" Ă%[)O= { +2 "valve0"D& +4 "carousel0"F% +10 "syringe0"B& +7 ."Plunge_home"Plunge.home.jog 0 +13 "syringe1"C% +6 C& +3 "syringe0"B% +3 "valve0"D ) +7 "valve1"E\ & +23 "core" ă&O> \ + Ƅ%M۹w % + …&׿0  + Æ%ݕb + Ç&uE ? + ˆ%ٝ_  + Ŋ&J*O ,= ĉ) +4 ."Lysis2_home"Lysis2.home.jog 0 +15 "valve0"D % +4 ."Air_home"Air.home.jog 0 +14 ."Carousel_home"Carousel.home.jog 0 +7 #"preston"-> exot +8 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +8 ."Toroid_home"Toroid.home.jog 0 +7 "syringe0"B ) +5 ."Archive_home"Archive.home.jog 0 +10 "syringe1"C ) +5 "carousel0"F) +10 "valve1"E* +9 "valve0"D* +6 "syringe1"C* +4 "syringe0"B* +13 "core" ċ%N[O< + Œ) + Í) + Ǝ) + ŏ* + Đ* Ñ* + ’* +2 "syringe0"B% +7 "syringe1"C& +6 "syringe0"B& +6 "valve1"E& +14 "valve0"D& +4 "syringe1"C% +6 ."Plunge_home"Plunge.in :homing +5 "valve0"D> (2 +9 "core" “%s٠bu  +2 Ô&z"k •&b׿(k  + Ŗ&EO4 + ė&O3 + Ø%ݖ`t +7 ."Plunge_home"Plunge.jog 582 +10 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +10 ."Toroid_home"Toroid.in :homing +8 ."PinPort_home"PinPort.in :homing +6 ."Archive_home"Archive.in :homing +8 ."Lysis1_home"Lysis1.in :homing +7 "valve0"D)F +10 "syringe0"B> (2 +3 "syringe1"C> -2 +4 "valve0"D?2 +2 "valve1"E?2 +2 "syringe0"B? -2 +5 "syringe1"C? (2 +5 ."Lysis2_home"Lysis2.jog 1619 +4 ."Air_home"Air.jog 1429 +10 "syringe0"B)S +14 ."PinPort_home"PinPort.jog 1225 +5 ."Lysis1_home"Lysis1.jog 1595 +8 ."Archive_home"Archive.jog 309 +10 "syringe1"C) +5 ."Toroid_home"Toroid.jog 1362 +7 "valve1"E* +6 "syringe1"C*; +8 "syringe0"B*5 +3 "valve0"D*R +12 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +11 ."Plunge_home"Plunge.seek :home +6 "valve0"D ' +26 "core" ž* + Ġ' +2 ."Plunge_home"Plunge.in :default +3 ."Archive_home"Archive.setRawPosition! 50 +7 "valve0"D> P ޤ-2 +7 "syringe0"B82 +9 ."Archive_home"Archive.seek :home +9 "syringe0"B!( +3 "core" š) + Û) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +7 ."Air_home"Air.setRawPosition! 0 +8 "syringe0"B72 +6 "syringe1"C7 +7 ."Lysis2_home"Lysis2.seek :home +5 ."Air_home"Air.seek :home +6 "syringe0"B"' +9 "syringe1"C#' +6 "core" Ý* + ¡( + ã' +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +5 "syringe1"C82 +8 ."Archive_home"Archive.in :default +8 ."Air_home"Air.in :default +7 ."Lysis1_home"Lysis1.seek :home +5 "syringe0"B?d PZ<2 +5 "syringe1"C>d P]z<2 +6 C$( +59 "core" Ŝ* +2 ¢' +3 ."PinPort_home"PinPort.setRawPosition! 3150 +2 ."Lysis2_home"Lysis2.in :default +9 "valve1"E8 N +5 "syringe0"B>d P\\k<2 +8 ."PinPort_home"PinPort.seek :home +7 "valve1"E%( +8 "core" ä( +3 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +141 "core" ğ* +5 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +10 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +6 "core" Ħ( +5 ."Toroid_home"Toroid.in :default +4 "valve0"D? ֜2 +245 "core" ť( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +39 ."clearToroid"Toroid.seek :clear +4 "valve1"E'& +5 "valve0"D(( +6 "core" ŧ&¼OR D +7 ."detached from han"PinPort.in :unlocking +3 "valve1"E? B2 +8 ."detached from han"PinPort.seek :clear +3 "valve1"E)( +47 "core" ũ( +286 Ĩ( +2 ."detached from han"Air.seek :clear +4 "syringe1"C*' +11 "core" ê' +2 ."detached from han"Lysis1.seek :clear +4 "syringe1"C+( +71 "core" ë( +2 ."detached from han"Lysis2.seek :clear +4 "syringe0"B,'j +82 "core" ¬' +2 ."detached from han"Archive.seek :clear +4 "syringe0"B-(j +80 "core" ­( +2 ."detached from han"Carousel.in :default +2 "carousel0"F><` F 22 +10 F.% +3 "core" Ʈ%1Q۹q  +4 ."detached from han"Carousel.home.seek 52 +6 "carousel0"F/'1 +17 "core" Ư' +2 "valve0"D0% +6 "core" İ%^OO !~ +5 #"detached from han"Selecting Cartridge 52 +2 "valve0"D1% +10 "core" ı%^OA !p +2 "syringe1"C2& +5 "core" ò&oW +2 "syringe1"C3% +5 "core" ó%ݫY +3 "valve1"E4& +4 "core" Ŵ&OH  +3 ."detached from han"Carousel.seek 52 +6 "carousel0"F5'1 +18 "core" Ƶ' +5 "carousel0"F6% +4 "core" ƶ%1+! 2 +3 ."detached from han"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +8 "syringe1"C7& +3 "core" ÷&hSy +4 ."detached from han"Lysis1.in :fast +2 "syringe1"C?d P[<2 +10 ."detached from han"Lysis1.seek :locked +7 "syringe1"C8( +248 "core" ø( +4 "valve0"D9% +6 "core" Ĺ%^O, !m +3 ."Plunge"Plunge.seek :locked +3 "valve0"D:'Z +14 "syringe1"C;% +6 "core" û%ݯҁ} +3 ."Air"Air.in :fast +3 "syringe1"C> P]z<2 +9 ."Air"Air.seek :locked +3 "syringe1"C<'6 +73 "core" ĺ' +32 ü' +2 ."detached from han"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +12 F=% +3 "core" ƽ%1O\  +7 #"detached from han"Powering cartridge bus +120 #"han"-> exit +4 #Session Ended +139 "cartridge_broadcast" ( +3 ."detached from han"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from han"PuckHeater.configure :default +2 "Cartridge" \ :D H  +9 ."detached from han"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" >t +4 "cartridge" t +2 "Cartridge" ?y +4 "cartridge" yVV +5 "Cartridge" @y  +5 "cartridge" y 1&0J59981 4&0{C3 +3 #"detached from han"Mounted lysis Cartridge board#J59981 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"DA% +8 "core" %ZG.OB . +2 "syringe1"CB& +4 "core" &Jf +3 "syringe1"CC% +4 "core" %6P P +3 "carousel0"FD% +4 "core" %1 \ +4 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 52 +4 "valve0"DE% +9 "core" %ZG/O? - +2 "syringe1"CF& +4 "core" &H^ +2 "syringe1"CG% +4 "core" %6 h S +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"GH& +9 "core" & +42 "carousel1"GI& +4 "core" & +46 "carousel1"GJ& +3 "core" & +47 "carousel1"GK& +3 "core" & +47 "carousel1"GL& +3 "core" & +47 "carousel1"GM& +3 "core" & +47 "carousel1"GN& +3 "core" & +47 "carousel1"GO& +3 "core" & +47 "carousel1"GP& +5 "core" &z +45 "carousel1"GQ& +5 "core" & +45 "carousel1"GR& +4 "core" &x +86 "carousel1"GS& +3 "core" & +7 "carousel1"GT& +15 "core" & +35 "carousel1"GU& +4 "core" & +4 "Can"i],z6tk  + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 23C (drifted 1%) +3 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +9 .PhaseSeparator.in :filling +2 "carousel1"G? +7 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GV, +6 "core" , +4 ."FILTERING"Gate.power :sampler,:ON +313 #"preston"-> exit +2 #Session Ended +373 "core" ,M +7 #"FILTERING"Head pressure reached 3.44psi in 8 seconds +3 ."sealing"Air.in :default +8 "carousel0"FW% +3 "syringe1"C>d P]z<2 +4 "core" %1Ne \  +6 #"FILTERING"Priming sample loop w/25ml, bypass w/10ml +4 .SamplePump.setPosition! 0ml +5 ."sealing"Air.seek :sealed +3 "sampler0"H7 +4 "syringe1"CX' +7 ."FILTERING"Bypass.open +6 "sampler1"IY1 +156 "core" 1 +73 "ambient0"JZ% +5 "core" %6 +2 ."FILTERING"Intake.open +3 "sampler0"H[1 +19 "core" ' +127 1 +3 "ambient0"J\\% +5 "core" %' +7 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +3 "sampler0"H]% +7 "core" %/ +44 "sampler0"H^% +3 "core" %5 +46 "sampler0"H_% +3 "core" %5 +47 "sampler0"H`% +4 "core" %- +46 "sampler0"Ha% +3 "core" %' +47 "sampler0"Hb% +3 "core" % +6 "Can"i],z6s/ + #"FILTERING"Vacuum tare is 54% of full scale at 23C (drifted 2%) +2 "ambient0"Jc% +8 "core" %+ +2 "ambient0"Jd% +4 "core" %9 +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"Je+ +5 "core" + +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?' +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"Gf, +5 "core" , +3 "sampler0"Hg% +6 "core" % +2 "sampler0"Hh% +4 "core" % +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "14:50:38.60" +4521 "Can"i],z6s1 +4 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n;#(@6t3 22Q +8 ."FILTERING"SamplePump.seek 10ml,1:10 +2 "sampler0"Hi'6 +57 ."pumpStarted"SamplePump.configure :prime +4 "sampler0"H#d }n;#(@6t3 22Q +1283 "core" ' +2 "sampler0"Hj% +5 "core" %6 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +5 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 ."FILTERING"Exhaust.open +3 "sampler0"Hk2 +150 "core" 2 +74 ."closeBypass"Bypass.close +4 "Can"n],5z3s2& +2 "sampler1"Il/ +154 "core" / +73 "sampler0"Hm% +3 "core" %F, +3 "ambient0"Jn% +4 "core" %sB +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"Jo+ +5 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gp, +7 "core" , +2 "ambient0"Jq% +4 "core" %uc +4 "sampler0"Hr% +5 "core" %( +3 "sampler0"Hs% +3 "core" %B +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n;#(@t3 22Q +8 ."FILTERING"SamplePump.seek 25ml,2:10 +3 "sampler0"Ht' +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n;#(@t3 22Q +742 #"benji"Session Ended +2515 "core" ' +2 "sampler0"Hu% +5 "core" %x 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +3 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +7 ."FILTERING"Bypass.open + "sampler1"Iv1 +148 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"Hw/ +147 "core" / +2 "carousel0"Fx% +4 "core" %1J \  +6 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :crack +2 "valve0"Dy( +168 "core" ( +77 ."FILTERING"Toroid.seek :cartridge +2 "valve0"Dz(m +206 "core" ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +3 ."FILTERING"Toroid.seek :clear +2 "valve0"D{( +370 "core" ( +3 "ambient0"J|% +3 "core" %z +5 "Can"n],5z3s9 e  +4 ."FILTERING"Abs.hold +2 "ambient0"J+ +3 "core" ʇ+ +4 ."FILTERING"PhaseSeparator.hold + "carousel1"G, +5 "core" dž, +3 "sampler0"H% +5 "core" ȅ%J +2 "sampler0"H% +5 "core" Ȅ%F +4 ."FILTERING"SamplePump.in :charge + "sampler0"H>n;u0B3 22Q +5 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +2 "sampler0"H' +1810 "core" ȃ'9 2 +5 "sampler0"H % +4 "core" ȋ%3y 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +5 ."FILTERING"Intake.close +2 "sampler0"H/ +146 "core" Ȗ/ +3 "ambient0"J% +4 "core" ʓ%% +2 #"FILTERING"Pressurized cartridge with 1.391ml to 14.2psi +2 #Isolated @28.5psia (13.1psi) +2 #Verifying cartridge holds at least 0.65..0.85 of 28.5psia +4 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +2 "ambient0"J% +5 "core" ʔ%! +46 "ambient0"J% +3 "core" ʘ% +47 "ambient0"J% +3 "core" ʕ% +47 "ambient0"J% +3 "core" ʗ% +47 "ambient0"J % +5 "core" ʉ%/ +45 "ambient0"J % +3 "core" ʌ%3 +47 "ambient0"J % +3 "core" ʍ%, +47 "ambient0"J% +4 "core" ʏ%' +46 "ambient0"J% +3 "core" ʑ%# +47 "ambient0"J% +3 "core" ʒ%! +3 "carousel0"F% +4 "core" Ɛ%1I \  +4 #"FILTERING"Sampling 100.0ml + .SamplePump.setPosition! 0ml +2 "sampler0"H7 +4 ."FILTERING"Exhaust.open +2 "sampler0"H2 +145 "core" ș2 +2 ."FILTERING"Intake.open +2 "sampler0"H1 +147 "core" Ȟ1 +4 "Can"n],5z3s?h r +2 "ambient0"J% +5 "core" ʚ% +16 "ambient0"J% +6 "core" ʛ% +3 ."FILTERING"Abs.in :default + "ambient0"J>: e  +4 ."FILTERING"Abs.hold +2 "ambient0"J+ +3 "core" ʝ+ +3 ."FILTERING"PhaseSeparator.hold + "carousel1"G, +4 "core" ǜ, +2 "ambient0"J'% +4 "core" ʧ% +3 "sampler0"H% +5 "core" ȟ%?  +2 "sampler0"H&% +5 "core" Ȧ%Y  +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n;#(@@B3 22Q +4 ."FILTERING"SamplePump.seek 100ml,6:04 +2 "sampler0"H%'# +62 ."pumpStarted"SamplePump.configure :sample +3 "sampler0"H#d n;#(@@B3 22Q +225 H.% +3 "core" Ȯ%\ !u 2 +3 "ambient0"J#% +5 "core" ʣ%= + #"sampling"Cartridge 52 filtering 2/100ml at 0.75ml/s with 10.3psi vacuum +3406 "Can"j\\,Lz1s}Jg. +2587 "sampler0"H0% +3 "core" Ȱ%b p 2 +3 "ambient0"J1% +4 "core" ʱ%v +2 #"sampling"Cartridge 52 filtering 47/100ml at 0.74ml/s with 9.0psi vacuum +5672 #"benjamin"Session Begun +234 #-> ls +82 "sampler0"H$% +3 "core" Ȥ%  2 +3 "ambient0"J2% +4 "core" ʲ% +2 #"sampling"Cartridge 52 filtering 91/100ml at 0.75ml/s with 7.5psi vacuum 3.7m deep (20.2psia) +1134 "core" ȥ' +2 "sampler0"H3% +5 "core" ȳ%#{ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +5 "carousel1"G6 +10 ."closeIntake"Intake.close +4 "sampler0"H4/ +7 ."closeExhaust"Exhaust.close +5 "carousel0"F6% +5 "sampler0"H70 +7 "core" ƶ%1[IO \ +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"D"( +112 "core" ȴ/ +16 ȷ0 +228 Ģ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :clear +3 "valve0"D!( +401 "core" ġ( +3 #"FILTERING"Sampled 100.0ml +3 "carousel0"F% +8 "core" Ǝ%1}K \ +7 "valve0"D/% +5 "core" į%ZGLO(  +2 "syringe1"C9& +5 "core" ù& ]\\ +3 "syringe1"C;% +5 "core" û%Zb y +3 "carousel0"F+% +4 "core" ƫ%1I \ +4 "carousel0"F=% +6 "core" ƽ%1}K \ +4 ."PROCESSING"Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C % +6 "core" à%Yd x +44 "syringe1"C*% +5 "core" ê%ZY { +45 "syringe1"C>% +3 "core" þ% Yb u +47 "syringe1"C?% +3 "core" ÿ%Tg ~ +47 "syringe1"C@% +3 "core" %bh | +4 ."sourcePumpShutdown"Gate.power :sampler,:OFF +43 "syringe1"CA% +3 "core" %^\\ +47 "syringe1"CB% +3 "core" %^@ | +47 "syringe1"CC% +3 "core" %^9 ~ +48 "syringe1"CD% +3 "core" %V7 | +47 "syringe1"CE% +6 "core" %Y/ | +44 "syringe1"CF% +3 "core" %V-  +47 "syringe1"CG% +3 "core" %VC } +47 "syringe1"CH% +3 "core" %~n +47 "syringe1"CI% +4 "core" %\ Vg v +46 "syringe1"CJ% +3 "core" % X` t +47 "syringe1"CK% +3 "core" %Vk q +47 "syringe1"CL% +3 "core" % Vn w +47 "syringe1"CM% +5 "core" %W\\ x +45 "syringe1"CN% +3 "core" %\ ZJ v +47 "syringe1"CO% +5 "core" %WK { +24 #"benjamin"-> showlog +21 "syringe1"CP% +3 "core" %S2 z +47 "syringe1"CQ% +4 "core" %\\; | +46 "syringe1"CR% +3 "core" %R< +4 "Can"j\\,Lz1ti ѵ + #"PROCESSING"Syringe tare is 50% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking +5 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +3 "valve1"ES( N +80 "core" ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +7 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 1250uL +5 ."posArchive"Archive.seek :empty +3 "syringe0"B>d P\\k<2 +5 "syringe1"CT( +4 "syringe0"BU( +6 ."posLyS2"Lysis2.seek :empty +15 "syringe0"BW' +2 "Can"i\\,\\z1s}LL  +231 "core" ( +580 ( +18 ' +2 "carousel0"FY% +4 "core" %1K \ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +4 .PinPort.seek :locked +4 "valve1"EZ( N +8 "core" ( +2 ."PROCESSING"Handoff.select :waste +4 .Plunge.seek :puckBottom +3 "valve0"D<'B +128 "core" ļ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C[% +8 "core" % +43 "syringe1"C\\% +4 "core" % +46 "syringe1"C]% +3 "core" % +47 "syringe1"C^% +4 "core" % +46 "syringe1"C_% +3 "core" % +47 "syringe1"C`% +3 "core" % +47 "syringe1"Ca% +3 "core" %o +47 "syringe1"Cb% +4 "core" %r +46 "syringe1"Cc% +5 "core" %k +45 "syringe1"Cd% +5 "core" %~ +45 "syringe1"Cg% +4 "core" %x +46 "syringe1"Ch% +4 "core" % +46 "syringe1"CV% +3 "core" % +47 "syringe1"Cj% +4 "core" % +46 "syringe1"Ck% +4 "core" % +46 "syringe1"Ce% +4 "core" % +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi + #Air stroke #1 of 1 +4 "syringe1"Cf% +6 "core" % +5 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +6 ."PROCESSING"Air.seek :pressurized +3 "syringe1"Cl' +518 "core" ' +2 "syringe1"Cm% +4 "core" %Sy < +2 #"PROCESSING"0.3psi after pressurize +3 .Air.in :compress +2 "syringe1"C>N  P]zP2 +7 ."PROCESSING"Air.seek :evac +3 "syringe1"Cn'| +534 #"benjamin"-> exit +3 #Session Ended +116 "core" ' +2 "syringe1"Cq% +6 "core" %|4y vP[ +4 #"PROCESSING"0.3psi after compress +2 "syringe1"Cr% +4 "core" %|4y ^ +47 "syringe1"Cs% +3 "core" %|4_ d +47 "syringe1"Ci% +3 "core" %|4e ` +19 '|4k U +4 "syringe1"Cu% +48 "core" %|4 T +3 #"PROCESSING"Reached 0.4 of 4.0psi after pumping 1775uL (1.00 strokes) +13 .Air.in :default +3 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.hold +2 "syringe1"Cv+ +4 "core" + +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Co% +4 "core" %|4 ^ +47 "syringe1"Cp% +3 "core" %|4u _ +47 "syringe1"Cw% +3 "core" %|4z ] +47 "syringe1"Cx% +3 "core" %|4v \\ +47 "syringe1"C(% +4 "core" è%|4 d +46 "syringe1"Cy% +3 "core" %|4} Z +47 "syringe1"Cz% +3 "core" %|4 M +47 "syringe1"C|% +3 "core" %|4k ^ +48 "syringe1"C}% +3 "core" %|4f S +47 "syringe1"C~% +3 "core" %|4I \\ +47 "syringe1"C% +3 "core" %|4F [ +47 "syringe1"C% +3 "core" Á%|4= d +47 "syringe1"C% +3 "core" Â%|4I ] +47 "syringe1"C\ % +3 "core" Ê%|4Q W +47 "syringe1"C% +4 "core" È%|4Y e +46 "syringe1"C% +2 "core" Å%|4S i +48 "syringe1"C% +3 "core" Ä%|4Z ` +47 "syringe1"Ct% +3 "core" %|4S p +47 "syringe1"C % +3 "core" Ë%|4] ] +47 "syringe1"C% +3 "core" Ö%|4^ ` +2 #"PROCESSING"Stabilized at 0.2psi after evac to waste +2 .Handoff.select :blocked + "valve0"D0 +48 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"D': +82 "core" ē' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi + #Air stroke #1 of 8 + "syringe1"C% +5 "core" Ô%|4{ s +3 ."PROCESSING"Air.in :compress + "syringe1"C>N  P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +3 "core" Ø' +3 "syringe1"C% +4 "core" Õ%|49 h +3 #"PROCESSING"0.9psi after compress +2 "syringe1"C% +4 "core" Ç%|4 f +47 "syringe1"C% +4 "core" Æ%|4 a +45 "syringe1"C% +4 "core" ×%|4{ f +46 "syringe1"C % +4 "core" É%|4{ g +46 "syringe1"C % +3 "core" Ì%|4| [ +47 "syringe1"C % +3 "core" Í%|4| h +47 "syringe1"C% +3 "core" Ï%|4z i +47 "syringe1"C% +3 "core" Ñ%|4x m +47 "syringe1"C% +3 "core" Ò%|4{ f +47 "syringe1"C% +3 "core" Ð%|4z ^ +47 "syringe1"C% +4 "core" Ù%|4{ g +46 "syringe1"C% +3 "core" Þ%|4 ` +47 "syringe1"C% +4 "core" Ú%|4w! d +46 "syringe1"C% +4 "core" Û%|4} ^ +46 "syringe1"C'% +4 "core" ç%|4x i +47 "syringe1"C% +3 "core" ß%|4} e +47 "syringe1"C&% +3 "core" æ%|4y ^ +47 "syringe1"C.% +5 "core" î%|4{ ^ +45 "syringe1"C#% +3 "core" ã%|4* ] +47 "syringe1"C% +3 "core" Ã%|4t _ +47 "syringe1"C0% +4 "core" ð%|4p d +46 "syringe1"C1% +4 "core" ñ%|4u b +6 #"PROCESSING"1.1psi after compress (stable) +2 .Air.in :gulp + "syringe1"C>N  P]z<2 +4 ."PROCESSING"Air.seek :topVent +2 "syringe1"C$'6 +636 "core" ä' +2 "syringe1"C2% +4 "core" ò%6& < +3 #"PROCESSING"0.9psi after gulp +2 "syringe1"C3% +4 "core" ó%6) < +46 "syringe1"C6% +5 "core" ö%6m +45 "syringe1"C4% +3 "core" ô%6l +47 "syringe1"C7% +3 "core" ÷%6h } +47 "syringe1"C% +3 "core" Ý%6e +48 "syringe1"C% +3 "core" Ü%6f +47 "syringe1"C{% +3 "core" %6g$ } +47 "syringe1"C"% +3 "core" â%6j# +47 "syringe1"C% +3 "core" Î%6k +47 "syringe1"C/% +3 "core" ï%6i +47 "syringe1"C9% +3 "core" ù%6h +47 "syringe1"C;% +4 "core" û%6g +2 #"PROCESSING"1.2psi after gulp (stable) + #Air stroke #2 of 8 +2 "syringe1"C+% +4 "core" ë%6p8 | +3 ."PROCESSING"Air.in :pressurize + "syringe1"C>@N  P]z<2 +4 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C=' +240 "core" ý'~9~ < +56 "syringe1"C % +6 "core" à%{hd +2 #"PROCESSING"Reached 1.1 of 20.0psi after pumping 2296uL (1.29 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."PROCESSING"Air.hold +2 "syringe1"C*+ +3 "core" ê+ +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D>'B +82 "core" ľ' +2 ."PROCESSING"delay 3 seconds +303 .Handoff.select :waste + "valve0"D +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C?% +4 "core" ÿ%{mcM +47 "syringe1"C@% +3 "core" %{p`H +47 "syringe1"CA% +3 "core" %{r^? +47 "syringe1"CB% +3 "core" %{pc7 +47 "syringe1"CC% +3 "core" %{nc= +47 "syringe1"CD% +3 "core" %{l\\P +47 "syringe1"CE% +3 "core" %{kZW +47 "syringe1"CF% +3 "core" %{m[f +47 "syringe1"CG% +6 "core" %{s`f +44 "syringe1"CH% +4 "core" %{i]k +46 "syringe1"CI% +5 "core" %{m`f +45 "syringe1"CJ% +5 "core" %{rb^ +45 "syringe1"CK% +4 "core" %{jYX +46 "syringe1"CL% +4 "core" %{m[S +3 ."PROCESSING"delay 1:00 +3928 "Can"l\\,jz2t| H +2074 ."PROCESSING"Handoff.select :blocked +2 "valve0"D0 +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi +2 "syringe1"CM% +3 "core" %{}o] +48 "syringe1"CN% +3 "core" %{ohp +47 "syringe1"CO% +3 "core" %{sil +47 "syringe1"CP% +3 "core" %{sdo +47 "syringe1"CQ% +3 "core" %{miW +47 "syringe1"CR% +3 "core" %{un? +47 "syringe1"C)% +3 "core" é%{le: +47 "syringe1"C8% +3 "core" ø%{ih3 +47 "syringe1"C-% +3 "core" í%{kh8 +47 "syringe1"C,% +4 "core" ì%{k_E +46 "syringe1"CY% +3 "core" %{n`V +47 "syringe1"C[% +3 "core" %{td] +47 "syringe1"C\\% +3 "core" %{mdf +47 "syringe1"C]% +3 "core" %{ux +47 "syringe1"CS% +3 "core" %{ydo +47 "syringe1"C^% +3 "core" %{xe^ +47 "syringe1"C:% +3 "core" ú%{ngg +47 "syringe1"C_% +3 "core" %{p`h +47 "syringe1"C`% +3 "core" %{oeV +47 "syringe1"Ca% +4 "core" %{mhE +46 "syringe1"Cb% +3 "core" %{kcN +47 "syringe1"Cc% +3 "core" %{{aL +47 "syringe1"Cd% +3 "core" %{l_J +47 "syringe1"Cg% +3 "core" %{l]N +47 "syringe1"Ch% +3 "core" %{i`; +47 "syringe1"CV% +3 "core" %{lbI +47 "syringe1"Cj% +3 "core" %{o]W +47 "syringe1"Ck% +3 "core" %{z]X +47 "syringe1"Ce% +3 "core" %{q`_ +47 "syringe1"Cf% +4 "core" %{r^X +46 "syringe1"CX% +3 "core" %{mf +47 "syringe1"Cm% +3 "core" %{i[a +47 "syringe1"Cl% +3 "core" %{oYd +47 "syringe1"Cq% +3 "core" %{i\\` +47 "syringe1"Cr% +3 "core" %{mYf +47 "syringe1"Cs% +3 "core" %{q\\[ +47 "syringe1"Ci% +3 "core" %{lX_ +47 "syringe1"Cu% +3 "core" %{r\\H +47 "syringe1"Co% +3 "core" %{vWM +47 "syringe1"Cp% +4 "core" %{qY< +47 "syringe1"Cw% +3 "core" %{iTD +47 "syringe1"Cx% +3 "core" %{mRC +47 "syringe1"C(% +2 "core" è%{tTJ +48 "syringe1"Cy% +3 "core" %{jPW +47 "syringe1"Cn% +3 "core" %{cMa +47 "syringe1"Cz% +3 "core" %{qQb +3 ."PROCESSING"Handoff.select :waste +2 "valve0"D +47 #"PROCESSING"0.1psi at evacEnd +4 .Plunge.seek :pTop +5 "valve0"D|': +83 "core" ' +2 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +2 "syringe1"C>d  P]z<2 +3 ."PROCESSING"Air.seek :fullVent +2 "syringe1"C}'݇ +1274 "core" ' +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :pBottom + "valve0"D~'B +82 "core" ' +2 #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +2 .Plunge.seek :locked +2 "valve0"D'Z +126 "core" ' +2 ."PROCESSING"Handoff.select :waste +2 "syringe1"C& +4 "core" Á&F @ +3 ."PROCESSING"Lysis1.in :slow +2 "syringe1"C?d P[<2 +12 ."PROCESSING"Lysis1.seek 950uL +2 "syringe1"C( +1402 "core" Â( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select :blocked +3 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"D\ ': +84 "core" Ċ' +2 "syringe1"C& +5 "core" È&zsS k +3 ."PROCESSING"Lysis1.seek 350uL +3 "syringe1"C( +2794 "core" Å( +2 ."PROCESSING"delay 3 seconds +303 "syringe1"C% +3 "core" Ä%݇/_N +4 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 + "syringe1"C> wk+ P]z<2 +4 Ct% +4 "core" %݇/!C +3 "syringe1"C & +4 "core" Ë&G_R  +40 "Can"l\\,\\z5t +7 ."Airmixer"Air.seek :evac +5 "syringe1"C'| +2 ."PROCESSING"Lysis1.seek 190uL +5 "syringe1"C( +754 "core" Ö' + Ô( +3 ."PROCESSING"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."PROCESSING"Air.seek :full +2 "syringe1"C'6 +646 "core" Õ' +3 ."PROCESSING"Lysis1.seek :empty +2 "syringe1"C( +893 "core" Ç( +2 "valve0"D% +5 "core" Ć%:7O? . +2 "syringe1"C% +3 "core" ×%6+ { +3 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.4psi +2 .Plunge.seek :puckBottom + "valve0"D 'B +83 "core" ĉ' +2 "valve0"D<% +5 "core" ļ%B(0V - +2 "syringe1"C % +5 "core" Ì%6| s +3 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @14.8psi +4 #Polling Can every 2:00 +51 "Can"j\\,[z1t\\g +3 ."PROCESSING"PuckHeater.configure :default #ambient 23.4C +2 "Cartridge" \ :D H  +3 ."PROCESSING"PuckHeater.seek 81.6C +4 "Cartridge" * +3 v9 +5 "cartridge" 9" +103 "Cartridge" 9 +3 "cartridge" 9"_d +104 "Cartridge" 9 +3 "cartridge" 9#sd +104 "Cartridge" 9 +5 "cartridge" 9& d +107 "Cartridge" 9 +3 "cartridge" 9)d +103 "Cartridge" 9 +4 "cartridge" 9-4d +103 "Cartridge" 9 +3 "cartridge" 90d +103 "Cartridge" 9 +4 "cartridge" 93d +103 "Cartridge" 9 +5 "cartridge" 96d +103 "Cartridge" '9 +3 "cartridge" 99n#d +103 "Cartridge" 9 +4 "cartridge" 9;'d +103 "Cartridge" &9 +4 "cartridge" 9>,d +103 "Cartridge" .9 +3 "cartridge" 9@70d +104 "Cartridge" #9 +3 "cartridge" 9B? 4d +103 "Cartridge" 9 +3 "cartridge" 9D8d +104 "Cartridge" 09 +3 "cartridge" 9E =d +103 "Cartridge" 19 +4 "cartridge" 9G Ad +103 "Cartridge" 9 +3 "cartridge" 9I$ Ed +105 "Cartridge" 29 +6 "cartridge" 9J8Jd +104 "Cartridge" 39 +3 "cartridge" 9L?8Nd +103 "Cartridge" 69 +4 "cartridge" 9M8Rd +103 "Cartridge" 49 +3 "cartridge" 9O8Wd +104 "Cartridge" 79 +3 "cartridge" 9P8[d +104 "Cartridge" 9 +4 "cartridge" 9Q8_d +103 "Cartridge" 9 +4 "cartridge" 9Secd +103 "Cartridge" {9 +3 "cartridge" 9T^ehd +104 "Cartridge" "9 +3 "cartridge" 9Ueld +103 "Cartridge" 9 +4 "cartridge" 9Vpd +103 "Cartridge" /9 +3 "cartridge" 9Xud +104 "Cartridge" 99 +3 "cartridge" 9YWyd +103 "Cartridge" ;9 +4 "cartridge" 9Z}d +103 "Cartridge" +9 +4 "cartridge" 9[d +103 "Cartridge" 9 +3 "cartridge" 9\\d +104 "Cartridge" $9 +4 "cartridge" 9]d +103 "Cartridge" =9 +4 "cartridge" 9^d +103 "Cartridge" 9 +3 "cartridge" 9`d +103 "Cartridge" ?9 +4 "cartridge" 9a d +108 "Cartridge" @9 +3 "cartridge" 9b2d +104 "Cartridge" A9 +5 "cartridge" 9cd +103 "Cartridge" B9 +3 "cartridge" 9d6d +104 "Cartridge" C9 +3 "cartridge" 9e'Dd +104 "Cartridge" D9 +3 "cartridge" 9f d +103 "Cartridge" E9 +4 "cartridge" 9g=Dc +103 "Cartridge" F9 +3 "cartridge" 9h8Dd +104 "Cartridge" G9 +3 "cartridge" 9i d +104 "Cartridge" H9 +3 "cartridge" 9j qd +105 "Cartridge" I9 +5 "cartridge" 9kd +103 "Cartridge" J9 +4 "cartridge" 9kqd +103 "Cartridge" K9 +4 "cartridge" 9ld +104 "Cartridge" L9 +3 "cartridge" 9mqd +104 "Cartridge" M9 +3 "cartridge" 9nd +104 "Cartridge" N9 +3 "cartridge" 9od +104 "Cartridge" O9 +3 "cartridge" 9pd +104 "Cartridge" P9 +4 "cartridge" 9q`d +104 "Cartridge" Q9 +47 "cartridge" 9rc +104 "Cartridge" R9 +3 "cartridge" 9sd +104 "Cartridge" )9 +3 "cartridge" 9t`d +103 "Cartridge" 89 +4 "cartridge" 9u;d +103 "Cartridge" -9 +4 "cartridge" 9v"d +103 "Cartridge" ,9 +4 "cartridge" 9vd +103 "Cartridge" Y9 +3 "cartridge" 9w$d +104 "Cartridge" [9 +3 "cartridge" 9xc +103 "Cartridge" \\9 +3 "cartridge" 9yO$ d +104 "Cartridge" ]9 +3 "cartridge" 9zE c +103 "Cartridge" S9 +4 "cartridge" 9{$d +112 "Cartridge" ^9 +3 "cartridge" 9{$d +103 "Cartridge" :9 +4 "cartridge" 9|Pc +105 "Cartridge" _9 +3 "cartridge" 9}uPd +104 "Cartridge" `9 +4 "cartridge" 9~OP#c +103 "Cartridge" a9 +4 "cartridge" 9~P'd +103 "Cartridge" b9 +4 "cartridge" 9}+d +103 "Cartridge" c9 +3 "cartridge" 9vP0c +104 "Cartridge" d9 +3 "cartridge" 9d}4c +104 "Cartridge" g9 +4 "cartridge" 90}8a +104 "Cartridge" h9 +3 "cartridge" 9=` +104 "Cartridge" V9 +3 "cartridge" 9A_ +103 "Cartridge" j9 +4 "cartridge" 9ME] +4 ."PROCESSING"PuckHeater.hold 2:00,81.6C +5 "Cartridge" k * +3 "cartridge" F +1228 "Can"g\\,^z2s1$\ , +4 g\\,^z2s1$\ , +10731 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +52 "Can"k\\,dz4t'G' +2 ."PROCESSING"PuckHeater.configure :default #ambient 23.5C +2 "Cartridge" \ :D H  +4 ."PROCESSING"PuckHeater.seek 80.6C +3 "Cartridge" e Y +3 f9 +5 "cartridge" 9] +5 ."PROCESSING"PuckHeater.hold 2:00,80.6C +5 "Cartridge" X Y +3 "cartridge"  +1147 "Can"l\\,`z3tL 'J +4 l\\,`z3t?>'J +10805 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +52 "Can"l\\,jz3t*heZ +3 ."PROCESSING"PuckHeater.configure :default #ambient 23.5C +2 "Cartridge" \ :D H  +3 ."PROCESSING"PuckHeater.hold 8:00,79.5C +5 "Cartridge" m  +1087 "Can"k\\,kz2tX*h +3 k\\,kz2tM*h +11962 j\\,]z2tN-1 +3 j\\,]z2tO-1 +11967 l]p,>z2tRj/ +4 l]p,>z2tP/ +11965 l^,)z4tRe2; +4 l^,)z4tN2; + ."ElfThermalWatch"PuckHeater.configure :default #ambient 23.0C +2 "Cartridge" \ :D H  +6 ."ElfThermalHold79.5C"PuckHeater.hold 1:50.01,79.5C +2 "Cartridge" l " +3 "cartridge"  +10962 +2 "syringe1"Cq% +3 "core" %6D u +3 #"PROCESSING"Lysate cooked @19.2psi +2 .Plunge.seek :puckTop + "valve0"Dr': +83 "core" ' +3 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi + #Air stroke #1 of 5 +2 "syringe1"Cs% +4 "core" %6 +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N   P]z<2 +3 ."PROCESSING"Air.seek :pressurized +2 "syringe1"Ci' +628 "core" ' +2 "syringe1"Cu% +4 "core" %S < +2 #"PROCESSING"17.9psi after pressurize +2 .Air.in :compress + "syringe1"C>N   P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"Co'| +227 "Can"h^N,z6sH4 +174 "core" 'p@{0 wP +5 "syringe1"Cp% +5 "core" % @ pP +2 #"PROCESSING"Reached 40.7 of 40.0psi after pumping 1633uL (0.92 strokes) + .Air.in :default +2 "syringe1"C>d P]z<2 +3 ."PROCESSING"Air.hold +2 "syringe1"Cw+ +3 "core" + +3 ."PROCESSING"delay 2 seconds +203 .Air.in :fast + "syringe1"C> P]z<2 +4 ."PROCESSING"Air.seek :full +2 "syringe1"Cx'6 +595 "core" ' +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D('B +82 "core" Ĩ' +3 "syringe1"Cy& +4 "core" &*Wcz  +3 ."PROCESSING"Lysis1.seek 138uL +2 "syringe1"Cn(# +651 "core" ( +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :puckTop +2 "valve0"Dz': +82 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +2 "syringe1"C>( +651 "core" þ( +2 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D*'B +82 "core" Ī' +2 "carousel0"F% +4 "core" Ɓ%1} \ +5 "valve0"D|% +4 "core" %B(KV - +5 "syringe1"C~% +7 ."PROCESSING"Lysis1.in :extract + "syringe1"C?d '\ J P[#2 +3 "core" %6[ t +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"CT( +4 C& +4 "core" È&2*R[ #\ +4 "syringe1"C& +3 "core" &*q # +16 "syringe1"C& +3 "core" Â& C+i$ # +14 "syringe1"C% +3 "core" Ä%6 v +4 "syringe1"Ct& +5 "core" &z - # +11 "syringe1"C & +3 "core" Ë& /= # +17 "syringe1"C}& +3 "core" &+ 1:@ # +10 "syringe1"C% +4 "core" Å%6 r +4 "syringe1"C& +3 "core" Ö&ޓ 3_ # +16 "syringe1"C& +3 "core" Ô& 5!M # +17 "syringe1"C& +3 "core" Æ&P 7 # +7 "syringe1"C% +57 "core" ×%6 r +2 "syringe1"C<& +8 "core" ü& = # +2 "syringe1"C & +5 "core" Ì& > # +5 "syringe1"Cv& +3 "core" & ? # +17 "syringe1"C& +3 "core" Ï&f"A_ # +11 "syringe1"C\ % +3 "core" Ê%6 | +3 "syringe1"C% +7 "core" Ñ%6? o +3 ."pump"Air.in :pressurize +2 "syringe1"C>@N g P]z<2 +3 C& +4 ."pump"Air.seek :pressurized +5 "syringe1"C' +3 "core" Ò&DZ5 # +5 "syringe1"C& +5 "core" Ù&^%F$ډ # +4 "syringe1"C& +3 "core" Þ&93F݉ #! +8 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 332uL during Lysis Syringe #1 (LyS1) move: @volumef332.17777777777775-:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 .Lysis1.in :default +2 "syringe1"C?d P[<2 +4 ."PROCESSING"Lysis1.seek 302uL +2 "syringe1"C( +3 "core" (i;G #! +95 Ú( +2 "syringe1"C% +4 "core" Û%9+ӊ_ q< +31 Ð')O < +5 "syringe1"C'% +7 "core" ç%lA\\ < + #"pump"Reached 12.7 of 15.5psi after pumping 371uL (0.21 strokes) +2 .Air.in :default +2 "syringe1"C>d P]z<2 +3 ."pump"Air.hold +2 "syringe1"C+ +3 "core" ß+ +6 "syringe1"C&% +4 "core" æ%:[ +3 "syringe1"C.% +4 "core" î%;[ +3 ."pump"Air.in :pressurize +2 "syringe1"C>@N g P]z<2 +5 ."pump"Air.seek :pressurized +3 "syringe1"C#' +464 "core" ã' +4 "syringe1"C% +5 "core" Ã%4S@ < +2 #"pump"12.5psi after pressurize +2 .Air.in :compressLysate +2 "syringe1"C>dN g P]z<2 +3 ."pump"Air.seek :evac +3 "syringe1"C0'| +215 "core" ð'&CPh < +4 "syringe1"C1% +5 "core" ñ%&Q < +2 #"pump"Reached 15.7 of 15.5psi after pumping 1437uL (0.81 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."pump"Air.hold +2 "syringe1"C+ +4 "core" Ø+ +3 "syringe1"C2% +3 ."PROCESSING"Lysis1.in :extract +8 "syringe1"C?d '\ J P[#2 +3 "core" ò%&PU +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"C3( +4 C6& +5 "core" ö&CS. # +3 "syringe1"C4& +4 "core" ô&@ CA # +4 "syringe1"C7& +3 "core" ÷&CT # +17 "syringe1"C& +3 "core" Ý& D4E # +5 "syringe1"C% +3 "core" Ü%&Q? +9 "syringe1"C{& +3 "core" &%E # +17 "syringe1"C"& +3 "core" â&^F # +17 "syringe1"C& +3 "core" Î&HGad #) +4 "syringe1"C/% +5 "core" ï%&QO +8 "syringe1"C9& +3 "core" ù&_H #, +17 "syringe1"C;& +3 "core" û&5mJP #% +17 "syringe1"C+& +3 "core" ë&K> # +4 "syringe1"C % +3 "core" à%&Q* +3 "syringe1"C$% +4 "core" ä%&QĊ +4 ."pump"Air.in :compressLysate + "syringe1"C>dN g P]z<2 +8 ."pump"Air.seek :evac +2 "syringe1"C='| +3 C& +4 "core" Ó&5Nr # +4 "syringe1"C?& +5 "core" ÿ&7o/Ozϊ2 # +7 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 437uL during Lysis Syringe #1 (LyS1) move: @volumef437.18730158730153/:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 .Lysis1.in :default +2 "syringe1"C?d P[<2 +5 ."PROCESSING"Lysis1.seek 407uL +2 "syringe1"C@( +4 "core" ó(-EQJs # +112 ( +2 "syringe1"CA% +4 "core" %O% r< +14 ý'0Nӌ < +4 "syringe1"CB% +5 "core" %.NҌl < +2 #"pump"Reached 15.5 of 15.5psi after pumping 1461uL (0.82 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."pump"Air.hold +4 "syringe1"CC+ +6 "core" + +3 "syringe1"CD% +5 "core" %N挍 +29 ."PROCESSING"Lysis1.in :extract + "syringe1"C?d '\ J P[#2 +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"CE( +5 CF& +3 "core" &Ll # +9 "syringe1"CG% +3 "core" %NԌ +5 "syringe1"CH& +3 "core" &tLՍ # +17 "syringe1"CI& +3 "core" &Lό # +17 "syringe1"CJ& +3 "core" &MMҌ # +6 "syringe1"CK% +3 "core" %NՌ +8 "syringe1"CL& +3 "core" &\ NՌ # +17 "syringe1"CM& +3 "core" &AO֌X #\ +17 "syringe1"CN& +3 "core" &~PҌ # +4 "syringe1"CO% +3 "core" %Nӌ +3 "syringe1"CP% +5 "core" %NЋ +3 ."pump"Air.in :compressLysate + "syringe1"C>dN g P]z<2 +6 ."pump"Air.seek :evac +3 "syringe1"CQ'| +5 CR& +4 "core" &+S?Ŋ # +3 "syringe1"C)& +4 "core" é&\ 6?S # +7 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 487uL during Lysis Syringe #1 (LyS1) move: @volumef486.75555555555553:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 .Lysis1.in :default + "syringe1"C?d P[<2 +6 ."PROCESSING"Lysis1.seek 457uL +2 "syringe1"C8(p +4 "core" (aJU # +91 'rFM] p< +6 "syringe1"C-% +4 "core" í%rMY u< +3 #"pump"Reached 16.0 of 15.5psi after pumping 1479uL (0.83 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +3 ."pump"Air.hold +3 "syringe1"C,+ +2 "core" ì+ +3 "syringe1"CY% +10 "core" ø( + %rMc +3 "syringe1"C[% +7 "core" %rMiǍ +2 ."PROCESSING"Lysis1.in :extract +2 "syringe1"C?d '\ J P[#2 +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"C\\( +4 C]& +7 "core" &p P # +10 "syringe1"CS& +3 "core" &yP̍O # +9 "syringe1"C^% +3 "core" %rM_ˍR +6 "syringe1"C:& +3 "core" ú&(QU΍Q # +16 "syringe1"C_& +3 "core" &R=ҍ # +17 "syringe1"C`& +3 "core" & So͌ # +6 "syringe1"Ca% +3 "core" %rM]ƌ +8 "syringe1"Cb& +3 "core" &=QTΌj # +17 "syringe1"Cc& +3 "core" &mVRȌ # +17 "syringe1"Cd& +3 "core" &xWNj # +4 "syringe1"Cg% +4 "core" %rMdȋ +3 "syringe1"Ch% +5 "core" %rM\\ϋY +2 ."pump"Air.in :compressLysate +2 "syringe1"C>dN g P]z<2 +4 CV& +3 ."pump"Air.seek :evac +5 "syringe1"Cj'| +5 "core" &IZF # +3 "syringe1"Ck& +4 "core" &?[,- # +13 "syringe1"Cf& +2 "core" &C]e # +11 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 584uL during Lysis Syringe #1 (LyS1) move: @volumef583.777777777777718:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 .Lysis1.in :default +2 "syringe1"C?d P[<2 +4 ."PROCESSING"Lysis1.seek 554uL +2 "syringe1"CX( +4 "core" (9zD_ # +128 ( +2 "syringe1"C % +4 "core" Í%K. w< +111 ';Il < +5 "syringe1"Ce% +4 "core" %Iw < +2 #"pump"Reached 15.6 of 15.5psi after pumping 1521uL (0.86 strokes) +2 .Air.in :default +2 "syringe1"C>d P]z<2 +4 ."pump"Air.hold +2 "syringe1"Cl+ +3 "core" + +4 "syringe1"Cq% +4 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d '\ J P[#2 +4 "core" %I +3 ."PROCESSING"Lysis1.seek 675uL,nil +4 "syringe1"Cs( +4 C & +5 "core" É& Y # +5 "syringe1"C& +4 "core" Õ&6YȌ # +4 "syringe1"Cu& +3 "core" &[Y% # +16 "syringe1"Cm& +3 "core" &"YrŒ # +4 "syringe1"Ci% +5 "core" %IŒ +8 "syringe1"Cp& +3 "core" &=Z # +17 "syringe1"Co& +3 "core" &dbZƌ # +17 "syringe1"Cw& +3 "core" &[̌ # +4 "syringe1"Cy% +4 "core" %IČU +9 "syringe1"Cr& +3 "core" &\\ƌ6 # +17 "syringe1"C& +3 "core" Ç&] # +17 "syringe1"C(& +3 "core" è&;_,Nj # +3 "syringe1"Cn% +4 "core" %I +3 "syringe1"C% +4 "core" Á%I +4 ."pump"Air.in :compressLysate + "syringe1"C>dN g P]z<2 +4 ."pump"Air.seek :evac +2 "syringe1"C|'| +4 C~& +3 "core" &Gaa # +5 "syringe1"C& +3 "core" È&4Ea@ # +6 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 648uL during Lysis Syringe #1 (LyS1) move: @volumef648.14603174603167+:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +4 .Lysis1.in :default + "syringe1"C?d P[<2 +4 ."PROCESSING"Lysis1.seek 618uL +3 "syringe1"C( +3 "core" (qGc6 # +114 ( +2 "syringe1"C% +6 "core" Â%b7H : o< +12 'X,G < +5 "syringe1"C% +7 "core" Ä%X`G| < + #"pump"Reached 15.6 of 15.5psi after pumping 1545uL (0.87 strokes) +2 .Air.in :default +2 "syringe1"C>d P]z<2 +3 ."pump"Air.hold +2 "syringe1"Ct+ +3 "core" + +3 "syringe1"C % +6 "core" Ë%XGG +32 ."PROCESSING"Lysis1.in :extract +2 "syringe1"C?d '\ J P[#2 +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"C}( +8 C& +2 "core" Å&^G # +5 "syringe1"C% +3 "core" Ö%XGÌ +10 "syringe1"C& +4 "core" Ô&'f^ # +16 "syringe1"C& +4 "core" Æ&7_ # +16 "syringe1"C& +2 "core" ×&P_ # +5 "syringe1"Cz% +5 "core" %XGŒ +8 "syringe1"C<& +3 "core" ü&r/`4t # +17 "syringe1"C & +3 "core" Ì&VaD # +17 "syringe1"Cv& +3 "core" &eaŋ # +4 "syringe1"C% +3 "core" Ï%XGË +3 "syringe1"C\ % +4 "core" Ê%XG} +4 ."pump"Air.in :compressLysate + "syringe1"C>dN g P]z<2 +4 ."pump"Air.seek :evac +3 "syringe1"C& + C'| +5 "core" Ñ& {cC # +8 ( +2 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +4 ."pump"Air.hold +2 "syringe1"C>+ +6 "core" Ò'L)Gm < + þ+ +3 "syringe1"C& +4 "core" Ù&ca +2 "syringe1"C% +4 "core" Þ%LGi +57 #"PROCESSING"Pulled 675uL lysate @14.5psi +3 "valve0"D% +4 "core" ě%B( OW +16 "syringe1"C'% +6 ."PROCESSING"Lysis1.seek 800uL,nil +4 "syringe1"C&(% +4 C.& +4 "core" ç%LG{ + î&cb # +3 "syringe1"Cx& +3 CT% +8 "core" & c[. # + %LGw +4 ."pump"Air.in :compressLysate +2 "syringe1"C& +5 C>dN g P]z<2 +6 "core" Ð&ci # + ."pump"Air.seek :evac +4 "syringe1"C'| +5 C& +4 "core" Ã&9*cT # +4 "syringe1"C1& +4 "core" ñ&D&c # +8 "syringe1"C2& +3 "core" ò& LGc # +17 "syringe1"C6& +3 "core" ö&*V*c # +17 "syringe1"C4& +3 "core" ô&5\\Gd> # +17 "syringe1"C7& +4 "core" ÷&Abi  { +17 "syringe1"CB& +3 "core" &/*i&" u +17 "syringe1"CD& +4 "core" &/Ci)J r +16 "syringe1"CF& +3 "core" &/Ei5U f +17 "syringe1"CG& +3 "core" &/Ki*h q +17 "syringe1"CH& +3 "core" &/Ii ~ +17 "syringe1"CI& +3 "core" &/Hi( s +17 "syringe1"C& +3 "core" Ø&/8i | +17 "syringe1"CJ& +3 "core" &/*i& u +17 "syringe1"CK& +3 "core" &/Ii | +17 "syringe1"CL& +3 "core" &/.i3 h +17 "syringe1"C3& +3 "core" ó&/i,ϋ o +17 "syringe1"CM& +3 "core" &1bi) #| +17 "syringe1"CN& +3 "core" &3di8 #w +17 "syringe1"CO& +4 "core" &84ciX׌G #p +16 "syringe1"CP& +3 "core" &@HTi$ #f +17 "syringe1"CR& +3 "core" &IVji #t +17 "syringe1"C)& +3 "core" é&Uhki #o +17 "syringe1"C=& +3 "core" ý&cyMj9 #h +17 "syringe1"C-& +3 "core" í&sdj" #a +17 "syringe1"C@& +3 "core" &lj- #f +17 "syringe1"CY& +3 "core" &kkJ #h +17 "syringe1"C[& +3 "core" &gk #c +17 "syringe1"C]& +3 "core" &hl" #d +17 "syringe1"CS& +3 "core" &dl #d +17 "syringe1"C^& +3 "core" &Nm #[ +17 "syringe1"C:& +3 "core" ú& sm #] +17 "syringe1"C_& +3 "core" æ( +2 &%on #[ +4 ."pump"Air.in :default + "syringe1"C>d P]z<2 +4 ."pump"Air.hold +2 "syringe1"C`+ +6 "core" ß'F<r d  P]z<2 +4 ."PROCESSING"Air.seek :fullVent +2 "syringe1"CC'݇ +253 "core" ' +2 ."PROCESSING"Handoff.select :waste + "valve0"D +48 #"PROCESSING"Recovered 800uL lysate @15.0psi +2 .delay 3 seconds +302 "syringe1"Cc% +4 "core" %݇/q +2 #"PROCESSING"After 3 delay Recovered 800uL lysate @0.8psi +2 "carousel0"Fd% +5 "core" %1) \ +5 "sampler0"Hg% +5 "core" %#G +4 ."closeHandoff"Handoff.select :close +4 "valve0"D0 +47 ."detached from han"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"EE& +2 "core" & N;O= +4 ."detached from han"PinPort.in :unlocking +2 "valve1"E? B2 +5 ."detached from han"PinPort.seek :clear +4 "valve1"Eh( +78 "core" ( +2 ."detached from han"Carousel.in :default + "carousel0"F><` F 22 +4 FV% +3 "core" %1hF \ +4 ."detached from han"Carousel.home.seek 52 +2 "carousel0"Fk'1 +14 "core" ' +2 "carousel0"Ff% +4 "core" %13 2 +4 "syringe1"CQ& +4 "core" &%nu U +3 ."detached from han"Plunge.seek :locked +2 "valve0"D8'Z +126 "core" ĸ' +2 "syringe0"B & +4 "core" &)c  +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +4 "syringe1"Ce% +6 ."Archive"Archive.seek :clear +2 "syringe0"B,(j +3 "core" %݇/h- +6 ."Air"Air.in :unlocking +2 "syringe1"Cq& +5 C> P]z-2 +3 "core" &%n U +55 ."Air"Air.seek :clear +7 "syringe0"B % +3 ."Lysis1"Lysis1.in :unlocking +6 "syringe1"C\\' +2 C? P[(2 +6 ."Lysis1"Lysis1.seek :clear +2 "syringe1"C( +3 "core" ‰%* +6 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bu'j +282 "core" Õ( +58 ¬( +90 ' +32 ' +3 "sampler0"Hm% +4 "core" %# +10 ."detached from han"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785352458.92EDT @EDT1785352490.79 "Can"m],.z3t8 +11964 k],%z6t90 +11967 l^X,z3t9: +11968 m^;,z3t:GX +11970 m],z4t:v +11971 m],z4t;\\ +11967 l],z2t; +8416 #"han"Session Begun +3553 "Can"m],z5t lysis +2852 #-> lysis=:lyseHold_phyto +1408 #-> start {fire lysis} +21 ."detached from han"Gate.power :sampler,:OFF,limit to 3500mA +5 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +265 "valve0"DP +3 Di +2 "core"  +7 ."detached from han"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from han"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."detached from han"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from han"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 ."detached from han"Abs.configure :default +2 "ambient0"J#: e  +10 ."detached from han"PhaseSeparator.configure :full +2 "carousel1"G$' +8 ."detached from han"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 ."detached from han"Air.configure :default +2 "syringe1"C#d P]z<2 +7 ."detached from han"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from han"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +34 ."detached from han"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from han"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +309 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !"detached from han"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'";/home/esp/esp2/utils/auv/cmd.rb:255:in `loadCartridge'",/home/esp/esp2/lib/msg.rb:68:in `retry'";/home/esp/esp2/utils/auv/cmd.rb:253:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"han:3:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +7 "valve0"Dp% +3 Do& +6 "core" %G#O;  +4 "carousel0"Fw% +6 "syringe0"By& +4 "syringe1"Cr% +6 C& +6 ."Plunge_home"Plunge.home.jog 0 +17 "syringe0"B(% +4 "valve1"En& +3 "valve0"D) +18 "core" &O- + %M+ % + &׾\\ ! + %ݖ\\ + Ç&p! D + ¨%ٞ]  + & O3 ,g ā) +9 "valve0"D~% +11 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +6 ."Carousel_home"Carousel.home.jog 0 +8 ."Air_home"Air.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +5 "valve1"E* +5 "syringe1"Cj* +4 "carousel0"FX) +3 "syringe1"C) +2 "syringe0"B* +6 "valve0"D * +2 "syringe0"B) +7 #"han"-> exit +15 #Session Ended +19 "core" %QG&O: + ň* * + Â) + ) „* + ċ* + …) +2 "syringe0"B% +13 "syringe1"C& +8 "valve0"Dl& +5 ."Plunge_home"Plunge.in :homing +3 "syringe0"B& +6 "valve1"E& +5 "syringe1"Cz% +5 "valve0"D> (2 +7 "core" –%t٫X} + Ô&d&  + &O4 + †&`׶V  + ŗ& O1  + %ݡ\\ +7 ."Archive_home"Archive.in :homing +9 ."Plunge_home"Plunge.jog 1481 +3 ."Lysis2_home"Lysis2.in :homing +3 ."Air_home"Air.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."Lysis1_home"Lysis1.in :homing +5 "syringe0"B? -2 +4 "valve0"D<) +3 "syringe0"B> (2 +3 "syringe1"C> -2 +8 "valve1"E?2 +2 "valve0"D?2 +2 "syringe1"C? (2 +3 ."Archive_home"Archive.jog 311 +9 ."Lysis2_home"Lysis2.jog 1617 +5 "syringe0"Bs*7 +3 B )Q +3 ."Air_home"Air.jog 1427 +14 ."PinPort_home"PinPort.jog 1228 +3 ."Lysis1_home"Lysis1.jog 1598 +3 "syringe1"Cv) +5 ."Toroid_home"Toroid.jog 1365 +8 "valve1"E* +2 "syringe1"C\ *> +5 "valve0"D*U +18 "core" * +3 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"B( +18 "core" ļ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +2 "valve0"D' +3 "core" ™( +4 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +14 "core" Ğ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +6 "core" Œ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +3 ."Lysis2_home"Lysis2.seek :home +2 "syringe0"B' +6 "core" ) +5 ."Air_home"Air.setRawPosition! 0 +4 "syringe1"C7 +48 ."Air_home"Air.seek :home +2 "syringe1"C|' +15 "core" ›' + Ê* + ' + ŏ* +3 ."PinPort_home"PinPort.setRawPosition! 3150 +4 "valve1"E8 N +4 ."Air_home"Air.in :default +5 ."Lysis2_home"Lysis2.in :default +6 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C>d P]z<2 +2 ."PinPort_home"PinPort.seek :home +3 "syringe0"B>d P\\k<2 +5 "syringe1"C82 +2 "valve1"E'( +6 ."Lysis1_home"Lysis1.seek :home +8 "syringe1"C.( +23 "core" î( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +197 "core" đ* +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +3 "valve0"D( +3 "core" ( +4 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +179 "core" ŧ( +7 ."PinPort_home"PinPort.in :default +3 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"ET& +2 "valve0"Dx( +10 "core" &OI 6 +5 ."detached from han"PinPort.in :unlocking +2 "valve1"E? B2 +3 ."detached from han"PinPort.seek :clear +3 "valve1"E( +40 "core" Ő( +293 ( +3 ."detached from han"Air.seek :clear +2 "syringe1"C' +4 "core" Ã' +3 ."detached from han"Lysis1.seek :clear +3 "syringe1"C1( +67 "core" ñ( +3 ."detached from han"Lysis2.seek :clear +2 "syringe0"B2'j +73 "core" ²' +3 ."detached from han"Archive.seek :clear +2 "syringe0"B6(j +74 "core" ¶( +2 ."detached from han"Carousel.in :default +2 "carousel0"F><` F 22 +4 F4% +6 "core" ƴ%1To  +4 ."detached from han"Carousel.home.seek 52 +2 "carousel0"F7'1 +15 "core" Ʒ' +3 "valve0"D#% +5 "core" ģ%^O9 !c +3 #"detached from han"Selecting Cartridge 22 +2 "valve0"Dt% +4 "core" %^O5 !{ +2 "syringe1"C& +5 "core" Ý&oY +3 "syringe1"C% +4 "core" Ò%ݣYo +2 "valve1"E& +4 "core" Ŝ&yO-  +4 ."detached from han"Carousel.seek 22 +3 "carousel0"F{' +632 "core" ' +5 "carousel0"F"% +3 "core" Ƣ%*[x 2 +3 ."detached from han"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"C& +3 "core" Î&kI +4 ."detached from han"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from han"Lysis1.seek :locked +2 "syringe1"C/( +247 "core" ï( +3 "valve0"D9% +7 "core" Ĺ%^O\\ !g +3 ."Plunge"Plunge.seek :locked +2 "valve0"D}'Z +7 "syringe1"C;% +11 "core" û%ݨGo +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +3 "syringe1"C+'6 +78 "core" ' +24 ë' +2 ."detached from han"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F>% +2 "core" ƾ%U[F  +7 #"detached from han"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from han"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from han"PuckHeater.configure :default #ambient 23.0C +2 "Cartridge" \ :D H  +4 ."detached from han"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y +3 "cartridge" y]'6(i +3 "Cartridge" $y 5 +4 "cartridge" y ?"UC\\q A"J60003 "dCb Bm/GyCg +4 #"detached from han"Mounted lysis Cartridge board#J60003 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3 "valve0"D% +4 "core" ē%ZGOI K +3 "syringe1"C?& +5 "core" ÿ& +2 "syringe1"C0% +4 "core" ð%6 d +3 "carousel0"F% +5 "core" ƚ%[ \ +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 22 +4 "valve0"DA% +4 "core" %ZGOA L +4 "syringe1"CB& +4 "core" & +2 "syringe1"CD% +5 "core" %6 t W +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi + "carousel1"GF& +6 "core" & +45 "carousel1"GG& +4 "core" & +46 "carousel1"GH& +3 "core" &\ +86 "carousel1"GI& +3 "core" &| +8 "carousel1"G& +3 "core" ǘ& +47 "carousel1"GJ& +3 "core" & +47 "carousel1"GK& +3 "core" &t +47 "carousel1"GL& +12 "core" &n\ +39 "carousel1"G3& +3 "core" dz&F +47 "carousel1"GM& +4 "core" &n +46 "carousel1"GN& +3 "core" &p +47 "carousel1"GO& +3 "core" &c +47 "carousel1"GP& +3 "core" & +47 "carousel1"GR& +3 "core" &\ +6 "Can"m],z5tq .= + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 23C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +2 .PhaseSeparator.in :filling +2 "carousel1"G? +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G), +3 "core" ǩ, +3 ."FILTERING"Gate.power :sampler,:ON +697 "core" ǩ,tO +7 #"FILTERING"Head pressure reached 3.52psi in 8 seconds +3 ."sealing"Air.in :default +3 "syringe1"C>d P]z<2 +3 "carousel0"F=% +4 "core" ƽ%[iMN \ +2 ."sealing"Air.seek :sealed +6 #"FILTERING"Priming sample loop w/25ml, bypass w/10ml + "syringe1"C-' +2 ."FILTERING"SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Bypass.open +2 "sampler1"I@1 +149 "core" 1 +73 "ambient0"JY% +5 "core" % +3 ."FILTERING"Intake.open +2 "sampler0"H[1 +27 "core" í' +122 1 +2 "ambient0"J]% +4 "core" % +4 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"HS% +4 "core" %5 +46 "sampler0"H^% +3 "core" %. +47 "sampler0"H:% +5 "core" Ⱥ%) +45 "sampler0"H_% +3 "core" %* +47 "sampler0"Ha% +3 "core" %( +47 "sampler0"Hb% +4 "core" % +5 "Can"m],z5s0> + #"FILTERING"Vacuum tare is 53% of full scale at 23C (drifted 2%) +2 "ambient0"J% +4 "core" ʟ% +3 "ambient0"J`% +8 "core" % +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +4 ."FILTERING"Abs.hold + "ambient0"Jc+ +3 "core" + +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?, +3 ."FILTERING"PhaseSeparator.hold +3 "carousel1"Gd, +2 "core" , +4 "sampler0"Hg% +4 "core" % +3 "sampler0"HE% +5 "core" % +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "15:31:27.97" +573 "Can"k],#z1s0> +3981 k],#z1s1@ +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m|#(@w3 22Q +4 ."FILTERING"SamplePump.seek 10ml,1:10 +2 "sampler0"HV'6 +62 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }m|#(@w3 22Q +1279 "core" ' +2 "sampler0"Hf% +4 "core" %6`2 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +5 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +4 ."FILTERING"Exhaust.open +2 "sampler0"HQ2 +146 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"I / +151 "core" ɍ/ +73 "sampler0"He% +3 "core" %[ +3 "ambient0"Jq% +4 "core" % +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +4 ."FILTERING"Abs.hold + "ambient0"J + +3 "core" ʉ+ +3 ."FILTERING"PhaseSeparator.hold +3 "carousel1"GZ, +3 "core" , +3 "ambient0"J5% +5 "core" ʵ%z +2 "sampler0"H*% +5 "core" Ȫ%W +3 "sampler0"HU% +3 "core" %Z +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m|#(@3 22Q +4 ."FILTERING"SamplePump.seek 25ml,2:10 +2 "sampler0"HC' +62 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }m|#(@3 22Q +3248 "core" ' +2 "sampler0"H&% +5 "core" Ȧ%ь& 2 +48 ."FILTERING"Abs.stop +5 "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +6 ."FILTERING"Bypass.open +2 "sampler1"IW1 +149 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"Hm/ +149 "core" / +2 "carousel0"Fi% +4 "core" %[K1 \ +6 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +4 "valve0"Dp( +168 "core" ( +77 ."FILTERING"Toroid.seek :cartridge +2 "valve0"Dn(m +206 "core" ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :clear +2 "valve0"D( +370 "core" ć( +2 "ambient0"Jr% +5 "core" % +3 "Can"k],#z1s;*EB +2 "ambient0"Jy% +4 "core" %} +2 "ambient0"Jw% +4 "core" %O +3 #"FILTERING"Pressurizing cartridge to 14psi above 14.2psia +2 .SamplePump.setPosition! 0ml + "sampler0"H7 +4 ."FILTERING"Intake.open +2 "sampler0"Ho1 +149 "core" 1 +3 ."FILTERING"Bypass.close + "sampler1"I(/ +151 "core" ɨ/ +2 ."FILTERING"Exhaust.close +2 "sampler0"H~0 +149 "core" 0 +2 "sampler0"H% +4 "core" ȗ%È +3 "ambient0"J% +4 "core" ʖ%p +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +3 ."FILTERING"Abs.hold +2 "ambient0"J+ +3 "core" ʔ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gz, +4 "core" , +3 "sampler0"H% +4 "core" Ȇ%È +3 "sampler0"Hl% +5 "core" % +4 ."FILTERING"SamplePump.in :charge + "sampler0"H>m|u0?3 22Q +5 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +2 "sampler0"H8' +994 "Can"k]m,2zAsC\\E  +540 "core" ȸ'xV 2 +6 "sampler0"Hh% +5 "core" %x4C 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +4 ."FILTERING"Intake.close +2 "sampler0"H/ +148 "core" ȕ/ +2 "ambient0"Jk% +5 "core" % +2 #"FILTERING"Pressurized cartridge with 0.998ml to 14.0psi +2 #Isolated @28.3psia (14.0psi) +3 #Verifying cartridge holds at least 0.65..0.85 of 28.3psia +3 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +2 "ambient0"J\\% +5 "core" % +46 "ambient0"J,% +3 "core" ʬ% +47 "ambient0"J!% +3 "core" ʡ% +47 "ambient0"Ju% +3 "core" % +47 "ambient0"J% +3 "core" ʁ% +47 "ambient0"J% +3 "core" ʄ% +47 "ambient0"J% +3 "core" ʅ% +47 "ambient0"J% +3 "core" ʂ%\ +47 "ambient0"J% +3 "core" ʈ% +47 "ambient0"Jj% +3 "core" % +3 #"FILTERING"Cartridge pressure fell 1% to 28.0psia +2 "carousel0"F % +5 "core" Ƌ%[kJ} \ +4 #"FILTERING"Sampling 100.0ml + .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Exhaust.open +4 "sampler0"Hs2 +144 "core" 2 +2 ."FILTERING"Intake.open +2 "sampler0"H<1 +145 "core" ȼ1 +4 "Can"k]m,2zAs?F +2 "ambient0"J % +4 "core" ʌ% +4 "ambient0"Jv% +5 "core" % +2 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +3 ."FILTERING"Abs.hold +2 "ambient0"J+ +3 "core" ʏ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G\ , +3 "core" NJ, +3 "ambient0"JT% +4 "core" % +4 "sampler0"H% +4 "core" ȑ%-  +3 "sampler0"H% +4 "core" %  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ m|#(@@3 22Q +63 ."FILTERING"SamplePump.seek 100ml,6:04 +2 "sampler0"H''# +5 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d m|#(@@3 22Q +223 H4% +3 "core" ȴ%5\ ' 2 +3 "ambient0"J#% +4 "core" ʣ% +2 #"sampling"Cartridge 22 filtering 1/100ml at 0.57ml/s with 13.7psi vacuum +5994 "sampler0"Ht% +4 "core" %o  2 +3 "ambient0"J% +5 "core" ʝ% +2 #"sampling"Cartridge 22 filtering 42/100ml at 0.67ml/s with 13.9psi vacuum +4073 "Can"j]p,:z C +3 "syringe1"C9& +4 "core" ù&M~ +2 "syringe1"C;% +4 "core" û%` +3 "carousel0"F1% +5 "core" Ʊ%[I \ +4 "carousel0"F>% +7 "core" ƾ%[J \ +3 ."PROCESSING"Handoff.select :waste +2 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"C% +3 "core" Þ%Qx +48 "syringe1"C% +3 "core" Ã% +47 "syringe1"C % +3 "core" à%L} +47 "syringe1"C$% +3 "core" ä%L~ +47 "syringe1"C% +3 "core" Ó%Qe +4 ."sourcePumpShutdown"Gate.power :sampler,:OFF +43 "syringe1"C?% +3 "core" ÿ%Ok +47 "syringe1"C0% +3 "core" ð%Ki +47 "syringe1"C% +3 "core" Ú%OL +48 "syringe1"CA% +3 "core" %Z +47 "syringe1"CB% +4 "core" %L\\ +46 "syringe1"CD% +3 "core" %Ii +47 "syringe1"CF% +3 "core" %Qi +47 "syringe1"CG% +3 "core" %G +47 "syringe1"CH% +3 "core" %L +4 "Can"j]p,:zd P\\k<2 +3 ."posArchive"Archive.seek :empty +4 "syringe1"C( +3 "syringe0"BJ( +5 ."posLyS2"Lysis2.seek :empty +9 "syringe0"BK' +241 "core" Ø( +578 ( +10 ' +2 "carousel0"FL% +4 "core" %([~ }\ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +6 .PinPort.seek :locked +4 "valve1"E3( N +3 "core" ų( +3 ."PROCESSING"Handoff.select :waste +2 .Plunge.seek :puckBottom +2 "valve0"DM'B +126 "core" ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CN% +4 "core" % +47 "syringe1"CO% +6 "core" % +43 "syringe1"CP% +3 "core" % +47 "syringe1"CR% +3 "core" % +47 "syringe1"C=% +3 "core" ý% +48 "syringe1"C@% +6 "core" % +43 "syringe1"CY% +3 "core" % +48 "syringe1"C+% +5 "core" ë% +45 "syringe1"C[% +3 "core" % +51 "syringe1"C]% +3 "core" % +43 "syringe1"CS% +3 "core" % +47 "syringe1"C^% +3 "core" % +47 "syringe1"C:% +3 "core" ú% +49 "syringe1"C_% +5 "core" % +13 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi + #Air stroke #1 of 1 + "syringe1"Ca% +6 "core" % +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N W P]z<2 +4 ."PROCESSING"Air.seek :pressurized +2 "syringe1"Cb' +514 "core" ' +2 "syringe1"C% +4 "core" ß%S΁ < +2 #"PROCESSING"0.4psi after pressurize +2 .Air.in :compress +2 "syringe1"C>N W P]zP2 +3 ."PROCESSING"Air.seek :evac +2 "syringe1"C`'| +651 "core" ' +2 "syringe1"Cg% +4 "core" %|4 wP +2 #"PROCESSING"3.0psi after compress +2 "syringe1"CE% +4 "core" %|4 sP +5 '|4v3 } +4 "syringe1"C)% +4 "core" é%|4 { +3 #"PROCESSING"Reached 3.0 of 4.0psi after pumping 1775uL (1.00 strokes) + .Air.in :default +2 "syringe1"C>d P]z<2 +3 ."PROCESSING"Air.hold +2 "syringe1"C%+ +3 "core" å+ +4 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"Cf% +5 "core" %|4k +46 "syringe1"CQ% +3 "core" %|4 +47 "syringe1"Cc% +5 "core" %|4* x +45 "syringe1"Cd% +3 "core" %|4 { +47 "syringe1"C % +3 "core" Í%|4 s +47 "syringe1"Ce% +3 "core" %|4 s +47 "syringe1"Cq% +3 "core" %|4 v +47 "syringe1"C5% +3 "core" õ%|4 { +48 "syringe1"C*% +3 "core" ê%|4 ~ +47 "syringe1"CU% +3 "core" %|4 v +47 "syringe1"CV% +3 "core" %|4u s +47 "syringe1"C&% +3 "core" æ%|4} x +47 "syringe1"CW% +3 "core" %|4i q +47 "syringe1"C % +3 "core" É%|4c +47 "syringe1"CZ% +3 "core" %|4S p +47 "syringe1"Cm% +3 "core" %|4|U r +47 "syringe1"Ci% +3 "core" %|4Q +47 "syringe1"Cx% +3 "core" %|4^ { +47 "syringe1"Cp% +3 "core" %|4~\\ +47 "syringe1"Cn% +3 "core" %|4~e | +47 "syringe1"Cr% +3 "core" %|4w p +47 "syringe1"Cy% +3 "core" %|4g k +47 "syringe1"Cw% +3 "core" %|4{p | +47 "syringe1"Co% +3 "core" %|4k y +2 #"PROCESSING"Stabilized at -0.0psi after evac to waste +2 .Handoff.select :blocked +2 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +3 "valve0"D(': +82 "core" Ĩ' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi + #Air stroke #1 of 8 + "syringe1"C~% +4 "core" %|4{3 n +4 ."PROCESSING"Air.in :compress + "syringe1"C>N  P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +3 "core" ×' +3 "syringe1"C% +4 "core" Ö%|48 y +3 #"PROCESSING"1.1psi after compress + "syringe1"C% +5 "core" Æ%|4~Q u +46 "syringe1"Cl% +3 "core" %|4|D s +47 "syringe1"CC% +3 "core" %|4yJ u +47 "syringe1"Ch% +4 "core" %|4G h +46 "syringe1"C% +3 "core" Õ%|4{=  +47 "syringe1"Ck% +3 "core" %|4yH  +47 "syringe1"C\\% +3 "core" %|4yN r +47 "syringe1"C,% +3 "core" ì%|4wR } +47 "syringe1"C!% +3 "core" á%|4{L f +47 "syringe1"C% +5 "core" Ô%|4R  +45 "syringe1"Cz% +3 "core" %|4N s +47 "syringe1"Cu% +3 "core" %|4xP +47 "syringe1"C% +3 "core" Á%|4zD x +47 "syringe1"C% +4 "core" Ä%|4sV t +46 "syringe1"C% +3 "core" Å%|4uP w +47 "syringe1"C% +3 "core" Â%|4tT +48 "syringe1"C% +4 "core" È%|4tS e +46 "syringe1"Cj% +3 "core" %|4pa u +47 "syringe1"C % +3 "core" Ë%|4s\\ m +47 "syringe1"Cs% +3 "core" %|4ta x +47 "syringe1"C<% +3 "core" ü%|4pl y +47 "syringe1"C % +3 "core" Ì%|4pt p +2 #"PROCESSING"1.3psi after compress (stable) +3 .Air.in :gulp + "syringe1"C>N  P]z<2 +46 ."PROCESSING"Air.seek :topVent +14 "syringe1"Cv'6 +410 "Can"j]?,9zAs-sTZH +225 "core" ' +2 "syringe1"CT% +5 "core" %6.K <s +2 #"PROCESSING"1.2psi after gulp +2 "syringe1"C% +3 "core" Ñ%6)^ <x +49 "syringe1"C% +2 "core" %6h[ { +47 "syringe1"C4% +3 "core" ô%6lr z +47 "syringe1"C#% +3 "core" ã%6dw r +47 "syringe1"C8% +3 "core" ø%6e} v +47 "syringe1"Ct% +4 "core" %6gz w +47 "syringe1"C% +3 "core" Ý%6h y +47 "syringe1"C% +3 "core" Ò%6d t +47 "syringe1"C|% +3 "core" %6e r +47 "syringe1"C% +3 "core" Ü%6d | +47 "syringe1"C% +3 "core" Û%6h z +47 "syringe1"C.% +3 "core" î%6a u +2 #"PROCESSING"1.5psi after gulp (stable) + #Air stroke #2 of 8 +2 "syringe1"C% +4 "core" Ù%6c s +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +4 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C' +627 "core" Ï' +2 "syringe1"C\ % +4 "core" Ê%,S f < +2 #"PROCESSING"1.3psi after pressurize +2 .Air.in :compress +2 "syringe1"C>N  P]zP2 +3 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +651 "core" Ç' +2 "syringe1"CX% +4 "core" %|4 rP +2 #"PROCESSING"12.7psi after compress +2 "syringe1"C"% +4 "core" â%|4y tP +47 "syringe1"C% +3 "core" Î%|4͋l +47 "syringe1"C9% +3 "core" ù%|4Y  +47 "syringe1"C;% +3 "core" û%|4X { +47 "syringe1"C1% +3 "core" ñ%|4M | +47 "syringe1"C>% +3 "core" þ%|4U2 v +47 "syringe1"C% +3 "core" Þ%|4SP w +47 "syringe1"C% +3 "core" Ã%|4Xo s +47 "syringe1"C % +3 "core" à%|4R { +47 "syringe1"C$% +3 "core" ä%|4R~ +47 "syringe1"C% +4 "core" Ó%|4V~ o +46 "syringe1"C?% +3 "core" ÿ%|4V ~ +47 "syringe1"C0% +3 "core" ð%|4X v +47 "syringe1"C% +3 "core" Ú%|4N +47 "syringe1"CA% +3 "core" %|4V  +47 "syringe1"CB% +3 "core" %|4R x +48 "syringe1"CD% +3 "core" %|4N z +47 "syringe1"CF% +3 "core" %|4N n +46 "syringe1"CG% +4 "core" %|4N z +46 "syringe1"CH% +5 "core" %|4M z +45 "syringe1"C% +5 "core" Ð%|4K y +45 "syringe1"C/% +3 "core" ï%|4" v +49 "syringe1"C6% +4 "core" ö%|4F x +44 "syringe1"C2% +4 "core" ò%|4I t +2 #"PROCESSING"15.6psi after compress (stable) +5 .Air.in :gulp +3 "syringe1"C>N  P]z<2 +4 ."PROCESSING"Air.seek :topVent +2 "syringe1"CL'6 +549 "core" 'f < +5 "syringe1"CN% +4 "core" %#Z͌ < +2 #"PROCESSING"Reached 15.5 of 20.0psi after pumping 3551uL (2.00 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."PROCESSING"Air.hold +2 "syringe1"CO+ +3 "core" + +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"DP'B +83 "core" ' +2 ."PROCESSING"delay 3 seconds +302 .Handoff.select :waste + "valve0"D +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CR% +3 "core" %# +48 "syringe1"CI% +4 "core" %#F +46 "syringe1"C}% +3 "core" %#g +47 "syringe1"C=% +3 "core" ý%#= +47 "syringe1"C@% +3 "core" %#+ +47 "syringe1"CY% +3 "core" %# +47 "syringe1"C+% +3 "core" ë%# +47 "syringe1"C[% +3 "core" %# +47 "syringe1"C]% +4 "core" %# +46 "syringe1"CS% +5 "core" %# +45 "syringe1"C^% +5 "core" %#} +45 "syringe1"C:% +51 "core" ú%# +3 "syringe1"C_% +4 "core" %# +42 "syringe1"Ca% +5 "core" %# +45 "syringe1"C-% +4 "core" í%#} +46 "syringe1"C% +4 "core" ß%# +46 "syringe1"Cb% +5 "core" %#E +45 "syringe1"Cg% +4 "core" %#x +46 "syringe1"CE% +4 "core" %#x +46 "syringe1"C)% +4 "core" é%#{ +47 "syringe1"Cf% +2 "core" %# +3 ."PROCESSING"delay 1:00 +6011 .Handoff.select :blocked +2 "valve0"D0 +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi +2 "syringe1"CQ% +3 "core" %#I +48 "syringe1"Cc% +3 "core" %#6 +47 "syringe1"Cd% +3 "core" %#R +47 "syringe1"C % +3 "core" Í%#Z +47 "syringe1"Ce% +3 "core" %#r +47 "syringe1"C`% +3 "core" %#u +47 "syringe1"Cq% +3 "core" %#| +47 "syringe1"C5% +3 "core" õ%#v +47 "syringe1"C*% +3 "core" ê%#} +47 "syringe1"CU% +4 "core" %#z +46 "syringe1"CV% +3 "core" %#f +47 "syringe1"C&% +3 "core" æ%#f +46 "Can"j]C,=z4tVf +2 "syringe1"CW% +3 "core" %#i +46 "syringe1"C % +3 "core" É%#k +47 "syringe1"CZ% +3 "core" %#}_ +47 "syringe1"Cm% +3 "core" %#Z +47 "syringe1"Ci% +3 "core" %#_ +47 "syringe1"Cx% +3 "core" %#}b +47 "syringe1"Cp% +3 "core" %#}l +47 "syringe1"Cn% +4 "core" %#}o +46 "syringe1"Cr% +3 "core" %#l +47 "syringe1"Cy% +3 "core" %#f +47 "syringe1"Cw% +3 "core" %#|k +47 "syringe1"Co% +3 "core" %#|l +47 "syringe1"C~% +3 "core" %#zx +47 "syringe1"C% +3 "core" Ö%#y{ +47 "syringe1"C% +3 "core" Æ%#z_ +47 "syringe1"Cl% +3 "core" %#}` +47 "syringe1"CC% +3 "core" %#{^ +47 "syringe1"Ch% +4 "core" %#x[ +46 "syringe1"CM% +3 "core" %#uX +47 "syringe1"C% +3 "core" Õ%#wc +47 "syringe1"Ck% +3 "core" %#vf +47 "syringe1"C%% +3 "core" å%#wm +47 "syringe1"C\\% +3 "core" %#ur +47 "syringe1"C,% +3 "core" ì%#wr +47 "syringe1"C!% +3 "core" á%#tr +47 "syringe1"C% +3 "core" Ô%#rx +47 "syringe1"Cz% +3 "core" %#tu +47 "syringe1"Cu% +4 "core" %#ue +47 "syringe1"C% +3 "core" Á%#tc +47 "syringe1"C% +3 "core" Ä%#o[ +47 "syringe1"C% +4 "core" Å%#qM +46 "syringe1"C% +3 "core" Â%#o^ +46 "syringe1"C% +3 "core" È%#g +48 "syringe1"Cj% +3 "core" %#op +2 ."PROCESSING"Handoff.select :waste +2 "valve0"D +49 #"PROCESSING"-0.1psi at evacEnd +3 .Plunge.seek :pTop +2 "valve0"D ': +82 "core" ċ' +2 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +2 "syringe1"C>d  P]z<2 +6 ."PROCESSING"Air.seek :fullVent +4 "syringe1"Cs'݇ +1517 "core" ' +3 ."PROCESSING"delay 2 seconds +202 .Plunge.seek :pBottom +2 "valve0"D<'B +82 "core" ļ' +2 #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +2 .Plunge.seek :locked +2 "valve0"D 'Z +125 "core" Č' +2 ."PROCESSING"Handoff.select :waste +2 "syringe1"C& +4 "core" ×&LK D +4 ."PROCESSING"Lysis1.in :slow + "syringe1"C?d P[<2 +61 ."PROCESSING"Lysis1.seek 950uL +2 "syringe1"CT( +1403 "core" ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select :blocked +3 "valve0"D0 +49 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"D': +85 "core" đ' +2 "syringe1"C& +4 "core" &zF _ +4 ."PROCESSING"Lysis1.seek 350uL +2 "syringe1"C4( +2795 "core" ô( +2 ."PROCESSING"delay 3 seconds +303 "syringe1"C#% +3 "core" ã%݇/up +4 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 +2 "syringe1"C> wk+ P]z<2 +4 C8% +2 "core" ø%݇/9b +4 "syringe1"Ct& +9 "core" &Gyi " +45 ."Airmixer"Air.seek :evac +5 "syringe1"C'| +3 ."PROCESSING"Lysis1.seek 190uL +4 "syringe1"C( +752 "core" Ý' +4 Ò( +3 ."PROCESSING"Air.in :fast + "syringe1"C> P]z<2 +4 ."PROCESSING"Air.seek :full +3 "syringe1"C|'6 +646 "core" ' +2 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"C( +892 "core" Ü( +2 "valve0"D% +4 "core" ě%:7eO0 Q +3 "syringe1"C.% +4 "core" î%6A x +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @23.7psi +2 .Plunge.seek :puckBottom +2 "valve0"D'B +71 "Can"l]@,:z=t&#Z +11 "core" ę' +2 "valve0"Dv% +4 "core" %B|( XZ - +3 "syringe1"C\ % +4 "core" Ê%6Q w +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @20.2psi +2 #Polling Can every 2:00 +52 "Can"l]?,@z8tZO +3 ."PROCESSING"PuckHeater.configure :default #ambient 23.2C +2 "Cartridge" \ :D H  +4 ."PROCESSING"PuckHeater.seek 81.6C +4 "Cartridge"  w +2 X9 +5 "cartridge" 9#9~ +106 "Cartridge" "9 +3 "cartridge" 9"d +104 "Cartridge" 9 +3 "cartridge" 9$>~d +104 "Cartridge" 99 +5 "cartridge" 9' d +103 "Cartridge" ;9 +4 "cartridge" 9, d +103 "Cartridge" 19 +3 "cartridge" 908d +104 "Cartridge" >9 +3 "cartridge" 93d +104 "Cartridge" 9 +3 "cartridge" 97`d +104 "Cartridge" 9 +4 "cartridge" 9:eRd +104 "Cartridge" 9 +4 "cartridge" 9=$#d +103 "Cartridge" $9 +3 "cartridge" 9?'d +104 "Cartridge" 9 +3 "cartridge" 9A+d +103 "Cartridge" ?9 +4 "cartridge" 9D 0d +104 "Cartridge" 09 +3 "cartridge" 9E4d +104 "Cartridge" 9 +3 "cartridge" 9G8d +103 "Cartridge" A9 +3 "cartridge" 9Iz2tHd +6 k]?,>z2t8d +7874 "cartridge" +4 #"PROCESSING"Polling Can every 2:00 +51 "Can"k]@,9z,tf +3 ."PROCESSING"PuckHeater.configure :default #ambient 23.2C +2 "Cartridge" \ :D H  +4 ."PROCESSING"PuckHeater.hold 8:00,79.5C +4 "Cartridge"  gK +4021 "Can"k]2,;z2tP g +4 k]2,;z2tFgg +11966 k\\,Bz1tQj9 +3 k\\,Bz1tQj9 +11965 m]<,8z8tPl +3 m]<,8z8tJl +11966 i\\,Kz0tS=oC8 +3 i\\,Kz0tQaoC8 +7987 "cartridge" +3 "syringe1"Cl% +3 "core" %6eD x +2 #"PROCESSING"Lysate cooked @24.7psi +2 .Plunge.seek :puckTop +2 "valve0"DC': +82 "core" ' +3 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi + #Air stroke #1 of 5 +2 "syringe1"Ch% +4 "core" %6c v +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N D P]z<2 +3 ."PROCESSING"Air.seek :pressurized +2 "syringe1"CM' +628 "core" ' +2 "syringe1"C% +3 "core" Õ%S/ < +3 #"PROCESSING"23.2psi after pressurize +2 .Air.in :compress + "syringe1"C>N D P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"Ck'| +493 "core" 'p<k yP +5 "syringe1"C%% +4 "core" å%< xP +2 #"PROCESSING"Reached 41.1 of 40.0psi after pumping 1685uL (0.95 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."PROCESSING"Air.hold +2 "syringe1"C\\+ +3 "core" + +3 ."PROCESSING"delay 2 seconds +203 .Air.in :fast + "syringe1"C> P]z<2 +4 ."PROCESSING"Air.seek :full +2 "syringe1"C,'6 +614 "core" ì' +2 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D!'B +83 "core" ġ' +2 "syringe1"C& +4 "core" Ô&*cK +3 ."PROCESSING"Lysis1.seek 138uL +2 "syringe1"Cz(# +651 "core" ( +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :puckTop + "valve0"Du': +82 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +2 "syringe1"C( +651 "core" Á( +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D'B +82 "core" Ą' +2 "carousel0"F% +4 "core" ƅ%[ \ +5 "valve0"D% +3 "core" Ă%Bz(XS - +5 "syringe1"C% +6 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d 'CJ P[#2 +3 "core" È%6 u +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"Cj( +4 CP& +5 "core" &@*kW # +5 "syringe1"CO& +7 "core" & *ǣ # +10 "syringe1"C& +4 "core" ×& D+qQ # +12 "syringe1"C % +3 "core" Ë%6 w +4 "syringe1"C<& +3 "Can"j\\,Iz2tUrV +2 "core" ü&q "-ž # +12 "syringe1"C& +3 "core" Ø&  /D # +17 "syringe1"C& +3 "core" &( 1GƙF # +9 "syringe1"C % +4 "core" Ì%6 } +4 "syringe1"CT& +3 "core" &ފ 3< # +17 "syringe1"C#& +3 "core" ã& 5- # +51 "syringe1"C8& +3 "core" ø& :IÐ # +2 "syringe1"Ct% +8 Cs& +3 "core" %6s n + &/ ;~~ # +20 "syringe1"C4& +4 "core" ô& = # +3 "syringe1"C& +4 "core" Ý& >i` # +4 "syringe1"C& +4 "core" Ò& ?+ # +10 "syringe1"C& +3 "core" Û&9 @zW # +5 "syringe1"C.% +4 "core" î%6Ì t +8 "syringe1"Cv& +3 "core" & BFlw # +17 "syringe1"C\ & +3 "core" Ê&*D‹ #6 +17 "syringe1"CX& +3 "core" &_F #, +5 "syringe1"C"% +4 "core" â%6 w +3 "syringe1"C% +5 "core" Ñ%6d s +2 ."pump"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +3 C& +3 ."pump"Air.seek :pressurized +4 "syringe1"C9' +7 "core" Î&Hj #T +3 "syringe1"C;& +4 "core" û&K)I] #P +12 "syringe1"C1& +3 "core" ñ&Q7J; #T +17 "syringe1"C>& +3 "core" þ&|>K~ #] +17 "syringe1"C& +9 "core" Þ&9K #_ +11 "syringe1"C& +3 "core" Ã&PL #] +17 "syringe1"C & +3 "core" à&&L  R +17 "syringe1"C$& +3 "core" ä&%L X +17 "syringe1"C& +4 "core" Ó&+L U +16 "syringe1"C?& +3 "core" ÿ&3L b +17 "syringe1"C0& +3 "core" ð&3L S +17 "syringe1"C& +3 "core" Ú&2L Z +17 "syringe1"CA& +4 "core" &.L S +16 "syringe1"CB& +3 "core" &4L T +17 "syringe1"CD& +6 "core" &CL" P +14 "syringe1"CF& +3 "core" &>L Y +17 "syringe1"CG& +4 "core" &?L \\ +16 "syringe1"CH& +3 "core" &>L Y +17 "syringe1"C& +3 "core" Ð&>L S +17 "syringe1"C/& +3 "core" ï&JL" P +17 "syringe1"C6& +3 "core" ö&EL ^ +17 "syringe1"C2& +3 "core" ò&IL ] +17 "syringe1"C& +3 "core" Ç&JL  e +17 "syringe1"CN& +3 "core" &HL Z +17 "syringe1"CL& +5 "core" &OL U +15 "syringe1"C(& +3 "core" è&PL V +17 "syringe1"CR& +4 "core" &SL V +16 "syringe1"CI& +3 "core" &NL Y +17 "syringe1"C}& +3 "core" &OL S +17 "syringe1"C=& +3 "core" ý&\\L [ +17 "syringe1"C@& +3 "core" &XL V +17 "syringe1"CY& +3 "core" &\\L X +17 "syringe1"C+& +3 "core" ë&]L Y +6 ù' +2 "syringe1"C[% +4 "core" %(S < +2 #"pump"10.1psi after pressurize +2 .Air.in :compressLysate +2 "syringe1"C>dN  P]z<2 +3 ."pump"Air.seek :evac +2 "syringe1"C]'| +4 CS& +6 "core" &Lu X +4 "syringe1"C^& +5 "core" &d P]z<2 +3 ."pump"Air.hold +2 "syringe1"C+ +3 "core" Ñ+ +4 "syringe1"C% +4 "core" Î%? +8 ( +2 "syringe1"C;% +4 "core" û%? +4 ."PROCESSING"Lysis1.in :extract + "syringe1"C?d 'CJ P[#2 +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"C1( +5 C>& +3 "core" þ&/Wj # +9 "syringe1"C& +3 "core" Þ&W # +9 "syringe1"C% +3 "core" Ã%? +5 "syringe1"C & +3 "core" à&jX{ # +17 "syringe1"C$& +3 "core" ä&-1Y # +17 "syringe1"C& +3 "core" Ó&y[( # +5 "syringe1"C?% +3 "core" ÿ%?o +9 "syringe1"C0& +3 "core" ð& ] # +17 "syringe1"C& +3 "core" Ú&: ^ # +17 "syringe1"CA& +3 "core" & `B # +4 "syringe1"CB% +4 "core" %?: +3 "syringe1"CD% +5 "core" %? +3 ."pump"Air.in :compressLysate + "syringe1"C>dN  P]z<2 +3 CF& +4 ."pump"Air.seek :evac +6 "syringe1"C,'| +5 "core" ñ( + & cGa # +2 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +4 ."pump"Air.hold +2 "syringe1"CG+ +7 "core" ì'L>R < + + +3 "syringe1"CH& +4 "core" &=cIx +3 "syringe1"C% +4 "core" Ð%>_ +2 #"PROCESSING"Pulled 675uL lysate @15.9psi +2 "valve0"D/% +5 "core" į%B( \ OY +4 "syringe1"C6% +7 ."PROCESSING"Lysis1.seek 800uL,nil +2 "syringe1"C2(% +4 "core" ö%>aN +4 "syringe1"C% +4 CN& +5 "core" Ç%>; + & \ cGcM # +5 ."pump"Air.in :compressLysate +2 "syringe1"C>dN  P]z<2 +5 ."pump"Air.seek :evac +5 "syringe1"CL'| +5 C(& +3 "core" è&8?cY| # +3 "syringe1"CR& +4 "core" &=@ccM # +13 "syringe1"CI& +3 "core" &QTcwd # +17 "syringe1"C}& +3 "core" &*nEc # +17 "syringe1"C=& +3 "core" ý&:edo # +18 "syringe1"C@& +3 "core" &Mfd_T # +16 "syringe1"CY& +3 "core" &_bdیE # +17 "syringe1"C+& +3 "core" ë&tae6܌7 # +17 "syringe1"C[& +3 "core" &He݋ # +17 "syringe1"CS& +3 "core" &^f # +17 "syringe1"C^& +3 "core" &`f狎 # +17 "syringe1"C:& +3 "core" ú&iIgze # +54 "syringe1"C_& +3 "core" &gj # +4 "syringe1"Ca& +3 "core" &shjڊ # +3 "syringe1"C-& +4 "core" í&TokG܊ # +7 "syringe1"C& +4 "core" ß&"Jk֊ # +16 "syringe1"Cb& +5 "core" &hlC # +15 "syringe1"Cg& +4 "core" &ajl # +16 "syringe1"CE& +5 "core" &sl܋ # +15 "syringe1"C)& +4 "core" é&UlB +16 "syringe1"Cf& +5 "core" &]l< +15 "syringe1"CQ& +4 "core" &\\lt +16 "syringe1"Cc& +4 "core" &]l +16 "syringe1"Cd& +4 "core" &Al +16 "syringe1"C & +5 "core" Í&Ml +15 "syringe1"C9& +3 "core" ù&\\l +17 "syringe1"Ce& +3 "core" &6l +17 "syringe1"C`& +3 "core" &lڋ # +17 "syringe1"Cq& +3 "core" &il،= # +17 "syringe1"C5& +4 "core" õ&jl֌d # +16 "syringe1"C*& +3 "core" ê&0Sl׌r # +17 "syringe1"CU& +3 "core" &Mpm'ьT #t +17 "syringe1"CV& +3 "core" &dNmBی # +15 ' o7KՌ < +12 "syringe1"C&% +4 "core" æ% c7Q͌3 +2 #"pump"Reached 14.9 of 15.5psi after pumping 1742uL (0.98 strokes) +3 .Air.in :default +2 "syringe1"C>d P]z<2 +4 CW& +2 ."pump"Air.hold +5 "syringe1"C + +3 "core" ò( +2 &%m\\ # +9 "syringe1"CZ& +4 "core" É+ + &%mb +3 "carousel0"Fm% +4 "core" %[C \ +5 ."PROCESSING"Air.in :glide +2 "syringe1"C>d  P]z<2 +3 ."PROCESSING"Air.seek :fullVent +3 "syringe1"Ci'݇ +199 "core" ' +2 ."PROCESSING"Handoff.select :waste +2 "valve0"D +47 #"PROCESSING"Recovered 800uL lysate @16.1psi +2 .delay 3 seconds +302 "syringe1"Cx% +3 "core" %݇/W +2 #"PROCESSING"After 3 delay Recovered 800uL lysate @1.9psi +2 "carousel0"Fp% +4 "core" %[B \ +7 "sampler0"Hn% +5 "core" %#x +4 ."closeHandoff"Handoff.select :close +4 "valve0"D0 +47 ."detached from han"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"Er& +3 "core" & N;IOD U +3 ."detached from han"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from han"PinPort.seek :clear +2 "valve1"Ey( +78 "core" ( +3 ."detached from han"Carousel.in :default + "carousel0"F><` F 22 +4 Fw% +3 "core" %[w \ +4 ."detached from han"Carousel.home.seek 22 +2 "carousel0"F~' +14 "core" ' +2 "carousel0"F% +4 "core" Ɩ%3[T 2 +3 "syringe1"C& +6 "core" Ï&%n"d R +3 ."detached from han"Plunge.seek :locked +2 "valve0"Do'Z +126 "core" ' +2 "syringe0"B& +3 "core" †&)Ӂ +6 ."Archive"Archive.in :unlocking +3 "syringe1"Cl% + "syringe0"B? PZ-2 +7 "core" %݇/a +2 ."Archive"Archive.seek :clear +5 "syringe0"Bh(j +4 ."Air"Air.in :unlocking +5 "syringe1"C& + C> P]z-2 +6 "core" Ù&%n| V +3 ."Air"Air.seek :clear +5 "syringe0"B% +4 ."Lysis1"Lysis1.in :unlocking +4 "syringe1"C|' +3 C? P[(2 +8 "core" •%*Ł +3 ."Lysis1"Lysis1.seek :clear +4 "syringe1"CM( +5 ."Lysis2"Lysis2.in :unlocking +5 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B%'j +292 "core" ( +106 ( +29 ' +34 ¥' +3 "sampler0"Hk% +4 "core" %# +13 ."detached from han"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785354936.13EDT @EDT1785355004.09 "Can"l\\,Hz4tut +11964 l\\,6z3tu +11964 m\\,5z4tv5 +11965 k],,-z0tv +11964 l]S,'z1twL +11964 m\\,0z$tw\  +11964 n],+z/txb( +11968 l]K,Hz.txF +11968 m]N,_z1tyyd +11968 k\\,nz2tz +11966 k\\,{z/tz +11968 l\\f,z(t{ +11969 k\\;,z2t{ +11967 l[,z2t|2 +11969 l[,z3t| +11965 m]6,z0t}I6 +11965 kc,z3t}T +11965 m\\-z4t~^r +11964 mY-z%t~ +11965 lZ;,z(tt +11967 lZ-z2t +11966 kY-z$t +11965 m^C-)z.t +11961 mY--z8t& +11959 nXV-,z2t.D +11963 nX4-,z3tb @1785361060.25EDT = =tMAIN =mMAIN_scheduler @EDT1785361061.46 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +146 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +229 #>BREAK< ESP3 Gateway v1.44 for core bus +7 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +413 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +67 #Sea pressure (Abs) tare is 6% of full scale +292 "Can"o_J+zLu + o_J+zLu + #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"l_@+z4u +46 #"Tina"Session Begun +11920 "Can"j^+z5uk0 +9535 #"Tina"-> exit +3 #Session Ended +575 #"preston"Session Begun +569 #-> MainThread.exit +6 #mEXIT +5 !"preston"o:ThreadError: mesg"killed thread:@timestampu: Time ':bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +7 #Session Ended @1785361300.27EDT @1785361565.17EDT = =mMAIN_scheduler =tMAIN @EDT1785361566.18 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +24 "Can"o\\,z8u +342 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +301 "Can"o\\,z8u' +2 o\\,z8u' + #tPolling Can every 2:00 +52 "Can"j\\+z2u( +7678 #t-> ESP.configure +6 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +2 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +16 #Powering core bus +265 "valve0"DP +3 D +3 "core" ā +7 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +6 .tSamplePump.configure :default +2 "sampler0"H#^ #(22 +7 .tToroid.configure :default +2 "valve0"D$ ֜2 +6 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +7 .tAbs.configure :default + "ambient0"J#  +7 .tPhaseSeparator.configure :default +2 "carousel1"G$ +7 .tArchive.configure :default +2 "syringe0"B$d PZ<2 +6 .tAir.configure :default +2 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 .tPinPort.configure :default + "valve1"E$ B22 +5 #tAll elf controllers running firmware version 4.94 +30 .Gate.power :motor,:ON,limit to 5000mA +324 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +4 !to:AnalyticModule::Unknown: mesg"SPR:bt[ "D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +694 #-> SC.elf.signon +2846 "Can"k]+z3u  +866 #t-> exit +3 "sampler0"H% +3 "core" Ȃ%1 +8 #mEXIT @1785361704.46EDT @1785361835.53EDT = =mMAIN_scheduler =tMAIN @EDT1785361836.78 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +188 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +49 "Can"m^+, z9u\ j S +2 #t>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +369 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +74 #Sea pressure (Abs) tare is 6% of full scale +301 "Can"m^+, z9u\ T f + m^+, z9u\ T f + #tPolling Can every 2:00 +6 #Waiting for espclients... +47 "Can"l^7,z2u\ H h +52 #"tina"Session Begun +11914 "Can"l^,z5u\ \  +11963 k^, z4u\   +6956 #"tina"-> slots +5010 "Can"k],z1u\ $ - +7876 #"tina"-> slots [60,54,48,42,36,30,24,17,12,6]=>nil +433 #-> slot +3658 "Can"l\\,z4u\ K +4487 #"tina"-> slots [60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:800, lysed2:800}] +714 #-> slot +3474 #-> Cart.aspectsOf :standard, extraDilutions:[1], lysed:800, lysed2:800 +2743 #-> Cart.aspect +548 "Can"j\\_,z1u\ Fi +929 #"tina"-> ESP.shutdown +5 .Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +11023 "Can"j\\,z2u +11968 l],z2uh +11971 l^, z5u +11967 l^,z6u{ +11968 l^{+z4u +11967 m_,z0u +5012 #"tina"-> ESP.ready! +5 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +5 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +19 #Powering core bus +226 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +40 "valve0"DP +3 D +3 "core" ā +6 ."tina"Plunge.configure :default +3 "valve0"D# P ޤ-2 +9 ."tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."tina"Toroid.configure :default +2 "valve0"D$ ֜2 +14 ."tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."tina"Abs.configure :default + "ambient0"J#  +10 ."tina"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."tina"Archive.configure :default +3 "syringe0"B$d PZ<2 +8 ."tina"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +5 ."tina"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +8 ."tina"PinPort.configure :default +2 "valve1"E$ B22 +7 #"tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +5 "valve0"D% +8 "core" Ă%G O0  + "valve0"D& +5 "carousel0"F% +6 "syringe0"B& +6 "syringe1"C% +5 ."Plunge_home"Plunge.home.jog 0 +7 "syringe1"C& +6 "syringe0"B % +9 "valve0"D) +3 "valve1"E\ & +26 "core" ă&O.  +3 Ƅ%L[Q3  …&l  + Æ%ݞ) + Ç&f( N + ‰%ِ~  + Ŋ&'*N ,` Ĉ) +15 ."PinPort_home"PinPort.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +4 "valve0"D % +7 ."Archive_home"Archive.home.jog 0 +2 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +5 "valve1"E * +3 "syringe0"B ) +5 "syringe1"C) +3 "carousel0"F) +4 "syringe0"B* +2 "valve0"D* +4 "syringe1"C* +23 "core" ċ%HG`O,  + Ō* + ) + Î) đ* + * + Ò* + Ə) +2 "syringe0"B% +11 "syringe1"C& +4 "valve1"E& +4 "syringe0"B& +5 "valve0"D& +5 "syringe1"C% +4 ."Plunge_home"Plunge.in :homing +6 "valve0"D> (2 +6 "core" “%rٙe  + Ô&m,l  + Ŗ&O + •&i؁  + Ę&O + ×%ݛg +7 ."Plunge_home"Plunge.jog 1490 +8 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +5 ."Lysis1_home"Lysis1.in :homing +6 ."PinPort_home"PinPort.in :homing +5 ."Archive_home"Archive.in :homing +4 "valve0"D) +5 "syringe0"B> (2 +2 "syringe1"C> -2 +5 "valve0"D?2 +2 "syringe1"C? (2 +10 "valve1"E?2 +6 "syringe0"B? -2 +3 ."Lysis2_home"Lysis2.jog 1621 +7 ."Air_home"Air.jog 1428 +6 ."Lysis1_home"Lysis1.jog 1598 +5 ."PinPort_home"PinPort.jog 1229 +4 ."Toroid_home"Toroid.jog 1360 +4 ."Archive_home"Archive.jog 301 +6 "syringe0"B)U +5 "syringe1"C) +3 C*> +2 "valve1"E* +2 "valve0"D*P +2 "syringe0"B*- +46 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +4 "valve0"D ' +8 "core" Ÿ* +2 ."Archive_home"Archive.setRawPosition! 50 + "syringe0"B82 +6 ."Archive_home"Archive.seek :home +3 "syringe0"B!( +7 "core" Ġ' +4 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +12 "core" ¡( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +8 "core" š) + Û) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Air_home"Air.setRawPosition! 0 +3 "syringe0"B72 +2 "syringe1"C7 +14 ."Lysis2_home"Lysis2.seek :home +3 ."Air_home"Air.seek :home +3 "syringe0"B"' +6 "syringe1"C#' +11 "core" Ü* + ã' +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 ."Air_home"Air.in :default +4 "syringe1"C82 +2 C>d P]z<2 +9 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"C$( +57 "core" ŝ* + ¢' + ä( +5 ."PinPort_home"PinPort.setRawPosition! 3150 + ."Lysis1_home"Lysis1.in :default +5 ."Lysis2_home"Lysis2.in :default +3 "valve1"E8 N +4 "syringe1"C?d P[<2 +4 "syringe0"B>d P\\k<2 +5 ."PinPort_home"PinPort.seek :home +11 "valve1"E%( +163 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +7 "core" Ħ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +246 "core" ť( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +6 "valve1"E'& +5 "valve0"D(( +5 "core" ŧ&¤O% \\ +7 ."tina"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."tina"PinPort.seek :clear +3 "valve1"E)( +42 "core" ũ( +294 Ĩ( +2 ."tina"Air.seek :clear +3 "syringe1"C*' +12 "core" ê' +2 ."tina"Lysis1.seek :clear +3 "syringe1"C+( +69 "core" ë( +3 ."tina"Lysis2.seek :clear +2 "syringe0"B,'j +76 "core" ¬' +3 ."tina"Archive.seek :clear +3 "syringe0"B-(j +76 "core" ­( +2 ."tina"Carousel.in :default + "carousel0"F><` F 22 +6 F.% +3 "core" Ʈ%R[mQ  +4 ."tina"Carousel.home.seek 22 +5 "carousel0"F/' +17 "core" Ư' +2 "valve0"D0% +5 "core" İ%^qO1 ! +2 "valve0"D1% +4 "core" ı%^vO. ! +4 "sampler0"H2% +4 "core" Ȳ%6 +4 "valve0"D3& +5 "core" ij&O2  +2 "carousel0"F4% +4 "core" ƴ%N[Q.  +3 "ambient0"J5% +4 "core" ʵ%%X +3 "carousel1"G6& +5 "core" Ƕ&MQ2 +2 "syringe0"B7& +4 "core" ·&j[f  +3 "syringe1"C8% +5 "core" ø%ݔ_F +2 "syringe1"C9& +3 "core" ù&Mo +4 "syringe0"B:% +3 "core" º%jٗZT +4 "valve1"E;& +4 "core" Ż&WO4  +4647 "Can"l\\~,z/uL2; +511 #"tina"-> slots +2280 #-> Cart.aspects +5666 #-> start {fireAll "standard"} +49 #"detached from tina"Cartridge group: standard\ \ +8 "valve0"D<% +5 "core" ļ%^O0 !} +3 "valve0"D=& +4 "carousel0"F>% +9 "syringe0"B?& +4 "syringe1"C@% +7 CA& +6 "syringe0"BB% +4 "valve1"EC& +21 "core" Ľ&O+ # + ƾ%M[cQ#  + ¿&j<` F 22 +7 FJ% +3 "core" %O[Q!  +4 ."detached from tina"Carousel.home.seek 22 +4 "carousel0"FK' +18 "core" ' +3 "valve0"DL% +4 "core" %^}O3 ! +4 #"detached from tina"Selecting Cartridge 60 +2 "valve0"DM% +9 "core" %^rO) ! +2 "syringe1"CN& +5 "core" &Ym +3 "syringe1"CO% +3 "core" %ݙJ +4 "valve1"EP& +4 "core" &IO)  +3 ."detached from tina"Carousel.seek 60 +3 "carousel0"FQ'9 +797 "core" ' +5 "carousel0"FR% +3 "core" %9)"S 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"CS& +4 "core" &Vl +47 ."detached from tina"Lysis1.in :fast +4 "syringe1"C?d P[<2 +16 ."detached from tina"Lysis1.seek :locked +3 "syringe1"CT( +249 "core" ( +4 "valve0"DU% +7 "core" %^zOl ! +3 ."Plunge"Plunge.seek :locked +3 "valve0"DV'Z +7 "syringe1"CW% +7 "core" %ݗ +3 ."Air"Air.in :fast +3 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"CX'6 +78 "core" ' +27 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +8 FY% +3 "core" %9X!Q^  +8 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +4 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +3 "Cartridge" \ :D H  +8 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" Zt +6 "cartridge" t +2 "Cartridge" [y +9 "cartridge" y%FB(^Jv +2 "Cartridge" \\y ' +5 "cartridge" y ܉"MCu "J60008 %l/;CO9 +4 #"detached from tina"Mounted lysis Cartridge board#J60008 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +8 "valve0"D]% +4 "core" %ZG-O7 / +2 "syringe1"C^& +5 "core" & y +3 "syringe1"C_% +4 "core" %6[ l +2 "carousel0"F`% +5 "core" %9!P \ +3 "carousel0"Fa% +6 "core" %9!Pm \ +119 ."PROCESSING"SPR.command "read_sequences" +32 .SPR.command "stats_on" +10 "carousel0"Fb% +4 "core" %9!kPj \ +6 ."PROCESSING"SPR.command "start 0 1" +69 ."SPRlogger"SPR.start /var/log/esp/spr26jul2918h.spr.bz2 +195 #"PROCESSING"Waiting for baseline SPR stabilization +4 .Handoff.select :waste +4 "valve0"D +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"Cc% +6 "core" %6a j +44 "syringe1"Cd% +3 "core" %6i p +47 "syringe1"Ce% +3 "core" %6] h +47 "syringe1"Cf% +4 "core" %6g c +46 "syringe1"Cg% +4 "core" %6T n +46 "syringe1"Ch% +5 "core" %6z] j +45 "syringe1"Ci% +3 "core" %6= i +47 "syringe1"Cj% +3 "core" %68 k +48 "syringe1"Ck% +3 "core" %6|- u +47 "syringe1"Cl% +3 "core" %6s@ r +47 "syringe1"Cm% +3 "core" %6xB t +49 "syringe1"Cn% +4 "core" %6xM k +44 "syringe1"Co% +3 "core" %6zV r +47 "syringe1"Cp% +3 "core" %6{` i +5 "Can"l\\~,z/u0Y +2 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +4 .PinPort.in :locking +3 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +3 "valve1"Eq( N +83 "core" ( +3 ."posArchive"Archive.in :fast +7 ."posLyS2"Lysis2.in :fast +7 "syringe0"B?d PZ<2 +6 ."PROCESSING"Lysis1.seek 800uL +4 "syringe0"B>d P\\k<2 +5 "syringe1"Cr(% +5 ."posArchive"Archive.seek :empty +9 "syringe0"Bs( +9 ."posLyS2"Lysis2.seek 800uL +11 "syringe0"Bt'% +188 "Can"g\\,z1rgY +203 "core" ( +159 ' +264 ( +2 ."PROCESSING"delayUntil "18:26:31.69" +1257 #"tina"-> exit +3 #Session Ended +10076 "Can"k\\e,z5uC w +11967 j],z2uN& +11967 k],z4uL"N +11967 l^, z4uC%u +11014 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +4 Bu% +4 "core" %%n+B B +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 Cv& +3 "core" &%n"S e +42 ."Lysis2mixer"Lysis2.seek 550uL +5 "syringe0"Bw' +4 ."PROCESSING"Lysis1.seek 550uL +5 "syringe1"Cx( +874 "Can"g],z3s_s)- +2608 "core" ' +8 ( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +2 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +146 #SPR stabilized +2 "syringe1"Cy& +3 "core" &X. +4 "syringe0"Bz% +4 "core" %Y\ W +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +266 #Waiting up to 11:00 for SPR to enter state 1 +6368 "Can"j\\,\ z5u8?.Y +5396 ."PROCESSING"SPR.command "hold_on" +141 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B{% +3 "core" %Yq$ +5 "syringe1"C|& +5 "core" &Xh= +48 ."Lysis2mixer"Lysis2.seek 250uL +4 "syringe0"B}' +4 ."PROCESSING"Lysis1.seek 250uL +6 "syringe1"C~( +4175 "core" ' +9 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +299 #Waiting up to 1:00:00 for SPR to enter state 7 +360 "Can"j\\-,z4t.5+ +11964 l[,z4u7: I +11967 k\\f,z3u4=g +11967 k]\\,z>u1A +11967 j],z4u<D +11966 j],z4u7G +11969 m]P,z2u5K +11968 m\\,z3u9}O +11972 j[,z +11972 k\\@,#z1u$"{7 +11972 l\\H, z5u$U +11972 l[,z3u0Es +11971 lZ,z-u$R +11972 lZ, z4u$\  +11975 jZ,"z3u2I +11975 k[,"z1u4 +1392 #"PROCESSING"SPR analysis completed +60 #"SPRlogger"Ended log spr26jul2918h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +112 "carousel0"F% +3 "core" Ɔ%9"P4 \ +8 "sampler0"H% +4 "core" ȅ%P4 +5 ."closeHandoff"Handoff.select :close +3 "valve0"D +49 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E& +4 "core" ń& N;O2  +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" Ń( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F % +3 "core" Ƌ%9!HPU \ +4 ."detached from tina"Carousel.home.seek 60 +2 "carousel0"F'9 +15 "core" Ɠ' +2 "carousel0"F% +4 "core" Ɣ%9%S 2 +3 "syringe1"C& +4 "core" ×&ށ2U +3 ."detached from tina"Plunge.seek :locked +3 "valve0"D'Z +4 "core" ĕ' +2 "syringe0"B& +5 "core" ˜&)wl  +5 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +3 "syringe1"C% +10 ."Archive"Archive.seek :clear +2 "syringe0"B(j +3 "core" Ö%6:V +5 ."Air"Air.in :unlocking +3 "syringe1"C & +3 C> P]z-2 +7 "core" Í&ށ2M +3 ."Air"Air.seek :clear +5 "syringe0"B% +3 "syringe1"C' +5 ."Lysis1"Lysis1.in :unlocking +8 "syringe1"C? P[(2 +3 "core" Ž%ށ3~G +3 ."Lysis1"Lysis1.seek :clear +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe1"C ( +2 "syringe0"B> P\\k(2 +7 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B'j +60 "core" Ò' +331 ˆ( +41 Ì( +10 ‘' +3 "sampler0"H% +4 "core" Ȑ%6 +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785368094.58EDT @EDT1785368095.28 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +308 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +29 "valve0"DP +7 D +3 "core" ę +7 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default + "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +60 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default + "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D% +4 "core" ğ%GO/  +2 "valve0"D& +6 "carousel0"F% +8 ."Plunge_home"Plunge.home.jog 0 +7 "syringe0"B& +4 "syringe1"C% +3 "valve0"D') +4 "syringe1"C& +11 "syringe0"B&% +2 "valve1"E%& +28 "core" Ě&O,  + ƛ%N#Q8 t + œ&r  Ý%ݏo + Þ&JqT j + ħ) + ¦%ٓU  + ť&O , +8 "valve0"D.% +9 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +4 ."PinPort_home"PinPort.home.jog 0 +4 "syringe1"C1) +2 "syringe0"B0) +4 "carousel0"F#) +4 "syringe1"C2* +3 "valve0"D3* +2 "syringe0"B$* +3 "valve1"E4* +27 "core" Į%OGO- # + ñ) + °) ò* + ¤* + ƣ) + ij* Ŵ* +5 "valve1"E5& +7 "syringe0"B6% +7 "syringe1"C7& +10 C8% +4 "valve0"D9& +4 "syringe0"B:& +2 ."Plunge_home"Plunge.in :homing +14 "valve0"D> (2 +3 "core" ŵ&O" + ¶%r٨hi + ÷&Pog + ø%ݎoc + Ĺ&O +2 º&epr +11 ."Plunge_home"Plunge.jog 1489 +8 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Archive_home"Archive.in :homing +2 "valve0"D;) + ."Lysis1_home"Lysis1.in :homing +7 "syringe0"B> (2 + "syringe1"C> -2 +3 "valve0"D?2 +8 "valve1"E?2 +9 "syringe0"B? -2 + "syringe1"C? (2 +2 ."Lysis2_home"Lysis2.jog 1618 +3 ."Air_home"Air.jog 1431 +7 ."Toroid_home"Toroid.jog 1362 +9 ."PinPort_home"PinPort.jog 1239 +3 "syringe0"B")R +5 "syringe1"C!) +5 ."Archive_home"Archive.jog 304 +6 ."Lysis1_home"Lysis1.jog 1602 +3 "valve1"E<* +2 "valve0"D*R +4 "syringe0"BB*0 +2 "syringe1"C?*B +43 "core" Ļ) +3 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"DC' +6 "core" * +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"BA( +8 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +12 "core" ( +4 ."Archive_home"Archive.in :default + "syringe0"B?d PZ<2 +4 "core" á) + ¢) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Air_home"Air.setRawPosition! 0 + "syringe0"B72 +3 "syringe1"C7 +5 ."Lysis2_home"Lysis2.seek :home +4 ."Air_home"Air.seek :home +4 "syringe1"C@' +5 "syringe0"B>' +8 "core" ÿ* + ' +2 ."Air_home"Air.in :default +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C>d P]z<2 +2 C82 +7 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C=( +6 "core" ¾' +4 ."Lysis2_home"Lysis2.in :default + "syringe0"B>d P\\k<2 +4 "core" ż* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +4 ."PinPort_home"PinPort.seek :home +3 "valve1"ED( +4 "core" ý( +3 ."Lysis1_home"Lysis1.in :default + "syringe1"C?d P[<2 +256 "core" ď* + ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"DJ( +3 "core" ( +4 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +151 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +3 "valve1"EL& +2 "valve0"DM( +7 "core" &O +6 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +3 ."detached from tina"PinPort.seek :clear +3 "valve1"EN( +40 "core" ( +294 ( +4 ."detached from tina"Air.seek :clear +2 "syringe1"CO' +5 "core" ' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"CP( +67 "core" ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B('j +73 "core" ¨' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"B*(j +74 "core" ª( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +48 F+% +3 "core" ƫ%9M#MQ  +4 ."detached from tina"Carousel.home.seek 60 +2 "carousel0"F,'9 +16 "core" Ƭ' +2 "valve0"D-% +4 "core" ĭ%^O3 ! +14 #"detached from tina"Selecting Cartridge 54 + "valve0"D/% +5 "core" į%^O1 !y +2 "syringe1"CK& +5 "core" &Q^{ +3 "syringe1"CR% +3 "core" %ݙ\\ +4 "valve1"ES& +4 "core" &O/ 3 +3 ."detached from tina"Carousel.seek 54 +2 "carousel0"FU'3 +146 "core" ' +4 "carousel0"FW% +4 "core" %3(<S 2 +3 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"CG& +3 "core" &UW +5 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CY( +248 "core" ( +2 "valve0"D % +7 "core" Ġ%^O] !l +3 ."Plunge"Plunge.seek :locked +2 "valve0"DF'Z +6 "syringe1"CZ% +7 "core" %ݓUT +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +3 ."Air"Air.seek :locked +2 "syringe1"C['6 +83 "core" ' +20 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F\\% +3 "core" %3T<Q  +7 #"detached from tina"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default + "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" ]y +4 "cartridge" yF( c +3 "Cartridge" ^y ' +5 "cartridge" y Ps/J59921 $Ws/C" s/GCO +4 #"detached from tina"Mounted lysis Cartridge board#J59921 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D_% +4 "core" %ZG1O/ + +5 "syringe1"C`& +5 "core" &R| +3 "syringe1"Ca% +5 "core" %6Ue Z +2 "carousel0"Fb% +4 "core" %3<}Pc \ +4 "carousel0"Fc% +6 "core" %3<mPq \ +116 ."PROCESSING"SPR.command "read_sequences" +38 .SPR.command "stats_on" +7 "carousel0"Fd% +3 "core" %3<xPm \ +9 ."PROCESSING"SPR.command "start 0 1" +67 ."SPRlogger"SPR.start /var/log/esp/spr26jul2919h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +2 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Ce% +3 "core" %6My c +48 "syringe1"Cf% +3 "core" %6Lj _ +47 "syringe1"Cg% +3 "core" %6Pp e +47 "syringe1"Ch% +3 "core" %6Lx j +47 "syringe1"Ci% +4 "core" %6N ` +46 "syringe1"Cj% +3 "core" %6O Z +47 "syringe1"Ck% +3 "core" %6M a +47 "syringe1"Cl% +3 "core" %6P b +47 "syringe1"Cm% +5 "core" %6M b +45 "syringe1"Cn% +4 "core" %6N ` +47 "syringe1"Co% +3 "core" %6T} a +46 "syringe1"Cp% +4 "core" %6H} ^ +46 "syringe1"C)% +4 "core" é%6O` g +46 "syringe1"CT% +5 "core" %6Hh Y +7 "Can"k[,"z1u+ + #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +4 "valve1"EI( N +79 "core" ( +4 ."posArchive"Archive.in :fast +6 ."posLyS2"Lysis2.in :fast +5 "syringe0"B?d PZ<2 +2 ."PROCESSING"Lysis1.seek 800uL +5 "syringe0"B>d P\\k<2 +4 "syringe1"CH(% +3 ."posArchive"Archive.seek :empty +5 "syringe0"Bu( +7 ."posLyS2"Lysis2.seek 800uL +10 "syringe0"Bv'% +395 "core" ( +155 ' +269 ( +2 ."PROCESSING"delayUntil "19:45:33.40" +4009 "Can"j\\,z3uDI +11972 m\\,!z4uA+' +11975 k[, z3uG;E +11975 k[ ,z3u>f'c +11974 mZ,z2uAY +3295 #"bill"Session Begun +354 #-> showlog +2723 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +7 Bt% +3 "core" %%nzh f +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 Cr& +3 "core" &%mP u +46 ."Lysis2mixer"Lysis2.seek 550uL +7 "syringe0"By' +4 ."PROCESSING"Lysis1.seek 550uL +8 "syringe1"Cz( +3478 "core" ' +9 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +181 #SPR stabilized +2 "syringe1"C{& +5 "core" &Xvk +3 "syringe0"B|% +8 "core" %Yuy +3 ."PROCESSING"delay 20 seconds +1263 "Can"jZM,z1u8+ +739 ."PROCESSING"SPR.command "start 2 0" +303 #Waiting up to 11:00 for SPR to enter state 1 +10929 "Can"kZ,!z3u3p +836 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +4 "syringe0"Bw% +6 "core" %Yw\\ +3 "syringe1"Cx& +6 "core" &X< +46 ."Lysis2mixer"Lysis2.seek 250uL +5 ."PROCESSING"Lysis1.seek 250uL +5 "syringe0"B' +4 "syringe1"C( +4184 "core" ' +3 Á( +3 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +387 #Waiting up to 1:00:00 for SPR to enter state 7 +4785 "Can"k[J,(z3t.һ +11974 m[,#z4u3HQ +11970 l[,!z2u4  +11972 k[,z4u2X 5 +11971 k[1, z2u3G S +11973 kZ,z4u3 q +11974 k[O+z3u0 +11972 i\\A+z4u2 +8913 #"SPRlogger"SPRsummary:5.66RIU,233RIU,242RIU,358RIU after 1926s +3060 "Can"l\\+z9u$o8 +11973 l\\+z>u$qw +33 #"bill"Session Ended +11939 "Can"i\\=+zAu$L\  +11973 j[+zDu/\ % +11974 k[ +zPu$n\ C +11971 k[S+zAu0\ a +11968 m\\C+z5u8X\  +10800 "syringe1"C& +4 "core" Â&?MX  +2 "syringe0"B\ % +6 "core" Š%? +2 ."PROCESSING"delay 20 seconds +1153 "Can"k\\+z3u82\ \ +852 ."PROCESSING"SPR.command "start 2 0" +306 #Waiting up to 11:00 for SPR to enter state 1 +10813 "Can"k]6+z3u0\\\ +954 ."PROCESSING"SPR.command "hold_on" +192 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B}% +3 "core" %?v +4 "syringe1"C~& +5 "core" &?Qt  +52 ."Lysis2mixer"Lysis2.seek 100uL +4 ."PROCESSING"Lysis1.seek 100uL +3 "syringe0"B'ށ +3 "syringe1"C(ށ +2097 "core" †' +4 Å( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +342 #Waiting up to 1:00:00 for SPR to enter state 7 +6804 "Can"l\\+z2u3H\ +11972 l\\8+z2u0a\ +11969 l[+z5u0  +11968 k[+z3u2e 3 +11969 k\\$+z3u7" Q +11968 k\\+z4u5& o +11970 j]+z6u5* +11966 m].+z4u2- +11967 l\\+z2u$>1q +11969 l\\+z3u$$5 +11971 m[+z2u$G9  +11969 k[+z4u/`= # +11971 j\\+z4u$=A A +11971 l\\+z6u0BE _ +11971 l\\+zDu5Il } +8778 #"PROCESSING"SPR analysis completed +55 #"SPRlogger"Ended log spr26jul2919h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +123 "carousel0"F% +3 "core" Ƅ%3CPu \  +7 "sampler0"H % +3 "core" ȋ%~ +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +49 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E& +3 "core" Ŕ& N9O)  +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" ŗ( +3 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" Ƙ%3?(Ph \  +5 ."detached from tina"Carousel.home.seek 54 +2 "carousel0"F'3 +15 "core" Ɩ' +2 "carousel0"F % +5 "core" ƍ%31@T 2 +2 "syringe1"C& +5 "core" Î&ށ2l +3 ."detached from tina"Plunge.seek :locked +2 "valve0"Dq'Z +5 "core" ' +2 "syringe0"BQ& +4 "core" &)  +5 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +3 "syringe1"CV% +5 ."Archive"Archive.seek :clear +3 "syringe0"Bs(j +5 "core" %6b v +3 ."Air"Air.in :unlocking +4 "syringe1"CX& +2 C> P]z-2 +9 ."Air"Air.seek :clear +3 "syringe1"C' +2 "core" &ށ2w +6 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B % +3 "syringe1"C? P[(2 +7 "core" ‰%ށ3~b +3 ."Lysis1"Lysis1.seek :clear +6 ."Lysis2"Lysis2.in :unlocking +2 "syringe1"C( +4 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B'j +49 "core" Ç' +343 ( +39 Ð( +11 ™' +3 "sampler0"H% +5 "core" ȟ%6h +14 ."detached from tina"Gate.power :motor,:OFF + .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785372837.30EDT @EDT1785372837.86 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +322 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 D& +3 "core" Ħ +7 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +8 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +6 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +7 ."detached from tina"Abs.configure :default + "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default + "syringe1"C$d P[<2 +59 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +11 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D% +6 "core" Ğ%G$O0  +2 "carousel0"F% +4 "valve0"D& +6 "syringe0"B& +10 "syringe1"C% +4 ."Plunge_home"Plunge.home.jog 0 +17 "syringe1"C%& +4 "syringe0"B.% +4 "valve1"E5& +6 "valve0"D6) +14 "core" Ɯ%M@Q- W + ĝ&O,  + ›&j  Ú%ݎ+ + å&Sf a + ®%٢*  + ŵ&O1 - + Ķ) +9 "valve0"D7% +5 ."Lysis2_home"Lysis2.home.jog 0 +8 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +2 ."Toroid_home"Toroid.home.jog 0 +4 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +3 "syringe0"B8) +2 "syringe1"C:) +3 "carousel0"F9) +9 "syringe0"B* +7 "valve0"D* +4 "valve1"E* +3 "syringe1"C* +24 "core" ķ%RG0O.  + ¸) + ú) •* + ƹ) + đ* + Œ* Ó* +3 "syringe0"B % +12 "syringe1"CE% +5 C& +4 "valve0"D& +4 "valve1"E'& +2 "syringe0"B0& +6 ."Plunge_home"Plunge.in :homing +10 "valve0"D> (2 +5 "core" Œ%sٝi  +2 %݋/ + È&K,  + ă&O#  + ŧ&/O °&cn +8 ."Plunge_home"Plunge.jog 1479 +7 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."PinPort_home"PinPort.in :homing +3 ."Archive_home"Archive.in :homing +2 "valve0"D1) +2 ."Lysis1_home"Lysis1.in :homing +4 "syringe0"B> (2 +3 "syringe1"C> -2 +6 "valve0"D?2 +3 "valve1"E?2 +6 "syringe0"B? -2 +2 "syringe1"C? (2 +2 ."Lysis2_home"Lysis2.jog 1620 +5 ."Air_home"Air.jog 1431 +6 ."Toroid_home"Toroid.jog 1360 +12 "syringe0"B3)T +2 "syringe1"C4) +3 ."PinPort_home"PinPort.jog 1256 +7 ."Lysis1_home"Lysis1.jog 1603 +7 "valve0"D$*P +2 ."Archive_home"Archive.jog 307 +6 "valve1"E2* +4 "syringe1"C;*C +2 "syringe0"BB*3 +38 "core" ı) +3 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D"' +9 "core" * +3 ."Archive_home"Archive.setRawPosition! 50 + "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"B!( +6 "core" Ģ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +11 "core" ô) +3 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +3 "core" ¡( + ³) +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 ."Archive_home"Archive.in :default +3 ."Air_home"Air.seek :home +2 "syringe0"B72 +2 B?d PZ<2 +5 "syringe1"C?' +3 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"B<' +15 "core" û* + ÿ' +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Air_home"Air.in :default + "syringe1"C82 +3 C>d P]z<2 +4 ."Lysis1_home"Lysis1.seek :home +5 "syringe1"CL( +6 "core" ¼' +2 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +4 "core" Ų* +3 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +5 ."PinPort_home"PinPort.seek :home +2 "valve1"E( +3 "core" ( +5 ."Lysis1_home"Lysis1.in :default + "syringe1"C?d P[<2 +243 "core" Ĥ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"DJ( +3 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +163 "core" ŏ( +4 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +4 "valve0"DD( +3 "valve1"E+& +5 "core" ū&Q n +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E@( +41 "core" ( +294 ( +5 ."detached from tina"Air.seek :clear +4 "syringe1"C=' +4 "core" ý' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C-( +24 "Can"h\\]+z4t9MJ +43 "core" í( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B/'j +73 "core" ¯' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"BK(j +74 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 FR% +3 "core" %3S@EP  +4 ."detached from tina"Carousel.home.seek 54 +2 "carousel0"FS'3 +45 "core" ' +3 "valve0"D>% +4 "core" ľ%^O< !} +13 #"detached from tina"Selecting Cartridge 48 +2 "valve0"DA% +4 "core" %^uO: ! +3 "syringe1"CW& +5 "core" &WX +2 "syringe1"CG% +5 "core" %ݙ( +3 "valve1"E#& +4 "core" ţ&@O9  +4 ."detached from tina"Carousel.seek 48 +2 "carousel0"F,'- +144 "core" Ƭ' +4 "carousel0"F % +4 "core" Ơ%-'qS 2 +3 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"CZ& +3 "core" &e +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CP( +3 "core" Ƭ' -N!Q$  +3 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 48 + 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi$Q:@pastThresholdF: @voltagef15.019179185076093T: @currentiS: @type2F:@outPressi!:\ @lostF: @inPressi:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni-: @pastRLSF: @tagi1: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 Ƭ' +239 ( +3 "valve0"D\\% +8 "core" %^O ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"DC'Z +3 "syringe1"CO% +14 "core" %ݓR~ +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C]'6 +78 "core" ' +24 ' +3 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F^% +3 "core" %-UʹRQ  +7 #"detached from tina"Powering cartridge bus +265 "cartridge_broadcast" ( +2 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" _y +3 "cartridge" ylClC +4 "Cartridge" `y  +5 "cartridge" y 8&0J59994 <&0C[^ +3 #"detached from tina"Mounted lysis Cartridge board#J59994 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3 "valve0"Da% +3 "core" %ZFO/ b +3 "syringe1"Cb& +6 "core" & +2 "syringe1"Cc% +5 "core" %6S +2 "carousel0"Fd% +5 "core" %-ʹVPc \ +3 "carousel0"Fe% +6 "core" %-ʹP{ \ +132 ."PROCESSING"SPR.command "read_sequences" +33 .SPR.command "stats_on" +7 "carousel0"Ff% +3 "core" %-ʹxPZ \ +6 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26jul2920h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +2 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +5 "syringe1"Cg% +5 "core" %6 +45 "syringe1"Ch% +4 "core" %6L +46 "syringe1"Ci% +3 "core" %6O +47 "syringe1"Cj% +4 "core" %6K +47 "syringe1"Ck% +5 "core" %6M +44 "syringe1"Cl% +3 "core" %6N +47 "syringe1"Cm% +4 "core" %6I +46 "syringe1"Cn% +4 "core" %6I ~ +47 "syringe1"Co% +3 "core" %6H +47 "syringe1"Cp% +3 "core" %6K +47 "syringe1"C)% +3 "core" é%6B +47 "syringe1"CT% +3 "core" %6H +47 "syringe1"CN% +4 "core" %6F ~ +47 "syringe1"CY% +5 "core" %6F +6 "Can"h\\]+z4u.N%  +2 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +2 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"E*( N +78 "core" Ū( +4 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 800uL +2 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +6 "syringe1"C((% +3 ."posLyS2"Lysis2.seek 800uL +3 "syringe0"Bt( +10 Br'% +402 "core" è( +156 ' +275 ( +2 ."PROCESSING"delayUntil "21:04:36.14" +8576 "Can"j\\1+z6u@eQW +11976 k[,z4u@BT +11973 l[,z2u?EW +11970 k[,, zd2 P\\k<2 +3 Bv% +3 "core" %%n. S +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 CH& +3 "core" &%n- X +46 ."Lysis2mixer"Lysis2.seek 550uL +6 "syringe0"B{' +3 ."PROCESSING"Lysis1.seek 550uL +5 "syringe1"C|( +3479 "core" ' +7 ( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +195 #SPR stabilized + "syringe1"Cw& +3 "core" &XK +4 "syringe0"Bx% +7 "core" %Y~ +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +306 #Waiting up to 11:00 for SPR to enter state 1 +3529 "Can"k[,z4u2c O +8238 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"By% +4 "core" %Y d +6 "syringe1"Cz& +5 "core" &Xl +49 ."Lysis2mixer"Lysis2.seek 250uL +4 ."PROCESSING"Lysis1.seek 250uL +3 "syringe0"B' +6 "syringe1"C\ ( +3516 "Can"k[,z2swj{ m +662 "core" ‚' +6 Ê( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +349 #Waiting up to 1:00:00 for SPR to enter state 7 +9447 "Can"k[w, z2u1Qo +11971 l[ ,zEu0sK +11968 kZ, zFu5v +11970 kZ,\ z4u2zS +11974 l[`,zCu3+} +11973 k\\p,zQu8! +11971 m]u,zFu9? +11971 m],z4u2L] +4355 #"SPRlogger"SPRsummary:5.55RIU,236RIU,234RIU,344RIU after 1926s +7615 "Can"k]2,z3u${ +11973 k\\,z@u$* +11974 i\\1,z2u$ +11974 j[,z3u4P +11972 m[/,z5u$) +11974 jZ,z3u0 +11972 k[,z5u6_/ +6235 "syringe1"C}& +5 "core" &?eZ +2 "syringe0"B~% +7 "core" %?X +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +3413 "Can"l[6,z2u2 M +8355 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B% +3 "core" %?\\ +6 "syringe1"C& +3 "core" Á&?j[ +57 ."Lysis2mixer"Lysis2.seek 100uL +3 ."PROCESSING"Lysis1.seek 100uL +4 "syringe0"B'ށ + "syringe1"C (ށ +2098 "core" Ë( +2 „' +3 ."PROCESSING"delay 15 seconds +1246 "Can"k[f,z2t.pk +257 ."PROCESSING"SPR.command "hold_off" +342 #Waiting up to 1:00:00 for SPR to enter state 7 +11376 "Can"l[,z3u7 +11974 l[p,zIu3f +11973 k[,z@u1 +11973 l[v,z@u5 j +11973 j[0,zBu1 +11972 j[Z,zBu8Û +11975 m\\^,zBu0= +11972 l],\ zKu08ʝ[ +11974 l]O,z6u$Dοy +11974 i\\,zKu$ +11972 k\\p,z;u$H< +11973 l[,zHu$= +11971 k[,\ zEu$h +11975 k[,zAu32 +11974 l[y,zRu2r- +4151 #"PROCESSING"SPR analysis completed +55 #"SPRlogger"Ended log spr26jul2920h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +123 "carousel0"F% +3 "core" Ɣ%-ʹP$ \ +6 "sampler0"H% +5 "core" Ș%; +4 ."closeHandoff"Handoff.select :close +3 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E & +3 "core" ō& N;8O' f +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" Ŏ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 FQ% +3 "core" %-ʸP^ \ +4 ."detached from tina"Carousel.home.seek 48 +2 "carousel0"FV'- +15 "core" ' +2 "carousel0"FX% +4 "core" %-0ʸT 2 +3 "syringe1"C & +4 "core" É&ށ2 +3 ."detached from tina"Plunge.seek :locked +2 "valve0"DI'Z +5 "core" ' +2 "syringe0"BU& +5 "core" &)w| # +4 ."Archive"Archive.in :unlocking +4 "syringe1"CF% + "syringe0"B? PZ-2 +6 "core" %6|  +5 ."Archive"Archive.seek :clear +4 "syringe0"Bu(j +3 ."Air"Air.in :unlocking +8 "syringe1"C[& + C> P]z-2 +7 ."Air"Air.seek :clear +3 "syringe1"C' +2 "core" &ށ2 +6 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B% +5 "syringe1"C? P[(2 +5 "core" †%ށ3|i +2 ."Lysis1"Lysis1.seek :clear +11 ."Lysis2"Lysis2.in :unlocking +4 "syringe1"C( +2 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B&'j +44 "core" Å' +349 ( +41 ß( +8 ¦' +3 "sampler0"H% +4 "core" Ȟ%D +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785377579.98EDT @EDT1785377580.62 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +314 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +237 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +29 "valve0"DP +3 D5 +3 "core" ĵ +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +6 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default + "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +56 "carousel0"F#<` F 22 +11 ."detached from tina"Abs.configure :default + "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default + "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D.% +6 "core" Į%GO.  +2 "valve0"D%& +3 "carousel0"F% +6 "syringe0"B& +6 "syringe1"C% +5 ."Plunge_home"Plunge.home.jog 0 +20 "syringe1"C& +6 "syringe0"B7% +4 "valve1"E'& +3 "valve0"D ) +18 "core" ĥ&O- Ӂ + ƚ%MʹQ' 6 + ›&j  Ü%݉) + Ý&Sg a + ·%ْ+  + ŧ&[O, -, + Č) +9 "valve0"D% +5 ."PinPort_home"PinPort.home.jog 0 +8 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +2 ."Carousel_home"Carousel.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +3 "valve1"EE* +2 "syringe0"B0) +3 "syringe1"C) +10 "carousel0"Fq) +4 "valve0"D* +5 "syringe0"B* +3 "syringe1"C* +21 "core" Ĉ%IGO. + * + °) Ã) + ć* + ) + ™* Ö* +3 "syringe0"Bs% +10 "syringe1"CM& +5 "valve0"D& +5 "valve1"E6& +4 "syringe1"C8% +5 "syringe0"B& +4 ."Plunge_home"Plunge.in :homing +3 "valve0"D> (2 +7 "core" %pٙl + &O3  + Đ&O + Ŷ&&YO  + ø%݅f  + —&cg +10 ."Toroid_home"Toroid.in :homing +4 ."Plunge_home"Plunge.jog 1486 +5 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 "valve0"D?2 + ."PinPort_home"PinPort.in :homing +5 "syringe0"B> (2 +3 "valve0"D:) + "syringe1"C> -2 +4 C? (2 +2 "syringe0"B? -2 +5 "valve1"E?2 +6 ."Toroid_home"Toroid.jog 1377 +4 ."Lysis2_home"Lysis2.jog 1621 +5 "valve0"D*a +4 "syringe0"B)U +9 ."Air_home"Air.jog 1432 +14 "syringe1"C) +4 ."Lysis1_home"Lysis1.jog 1603 +6 ."Archive_home"Archive.jog 307 +4 ."PinPort_home"PinPort.jog 1247 +6 "syringe1"C*C +2 "valve1"EB* +4 "syringe0"B1*3 +43 "core" ĺ) +3 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +3 "valve0"D4' +6 "core" ±* +3 ."Archive_home"Archive.setRawPosition! 50 + "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"B3( +7 "core" Ĵ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +4 "core" ’) +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +5 ."Lysis2_home"Lysis2.seek :home +2 "syringe0"B;' +4 "core" Ó) + ³( +3 ."Air_home"Air.setRawPosition! 0 +3 ."Archive_home"Archive.in :default +2 "syringe1"C7 + "syringe0"B?d PZ<2 +8 ."Air_home"Air.seek :home +5 "syringe1"C2' +5 "core" Õ* + »' ò' +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Air_home"Air.in :default +2 ."Lysis2_home"Lysis2.in :default +2 "syringe1"C82 + C>d P]z<2 +2 "syringe0"B>d P\\k<2 +14 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C+( +12 "core" * +2 ."PinPort_home"PinPort.setRawPosition! 3150 + "valve1"E8 N +5 ."PinPort_home"PinPort.seek :home +2 "valve1"E$( +3 "core" ë( +6 ."Lysis1_home"Lysis1.in :default +3 "syringe1"C?d P[<2 +213 "core" đ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"DJ( +3 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +195 "core" Ť( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +50 "valve1"E& +4 "valve0"DR( +5 "core" ŏ&O +7 ."detached from tina"PinPort.in :unlocking +5 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E>( +41 "core" ž( +295 ( +4 ."detached from tina"Air.seek :clear +2 "syringe1"C?' +5 "core" ÿ' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"CA( +68 "core" ( +5 ."detached from tina"Lysis2.seek :clear +2 "syringe0"BL'j +74 "core" ' +2 ."detached from tina"Archive.seek :clear +3 "syringe0"BW(j +74 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 FG% +3 "core" %-VʺIQ\  +4 ."detached from tina"Carousel.home.seek 48 +2 "carousel0"F#'- +15 "core" ƣ' +2 "valve0"D<% +3 "core" ļ%^O9 !d +8 #"detached from tina"Selecting Cartridge 42 +2 "valve0"D!% +5 "core" ġ%^O: !~ +2 "syringe1"C & +5 "core" à&[X +2 "syringe1"CZ% +5 "core" %ݓX +3 "valve1"E9& +5 "core" Ź&O? 3 +3 ."detached from tina"Carousel.seek 42 +3 "carousel0"FS'( +144 "core" ' +5 "carousel0"F\\% +4 "core" %(*?S 2 +2 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"CO& +5 "core" &`W +3 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +4 "syringe1"C-( +247 "core" í( +3 "valve0"D^% +8 "core" %^~O ! +2 ."Plunge"Plunge.seek :locked +3 "valve0"D"'Z +3 "syringe1"C=% +8 "core" ý%ݑ[^ +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C_'6 +85 "core" Ģ' +18 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F`% +3 "core" %(Z0cQ  +8 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +4 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" ay +3 "cartridge" yFF +3 "Cartridge" by  +5 "cartridge" y }ߛ/J59976 / CMh +3 #"detached from tina"Mounted lysis Cartridge board#J59976 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"Dc% +3 "core" %ZGO( [ +2 "syringe1"Cd& +6 "core" &O +2 "syringe1"Ce% +5 "core" %6R +2 "carousel0"Ff% +5 "core" %(/PV \  +3 "carousel0"Fg% +6 "core" %(/Pe \  +116 ."PROCESSING"SPR.command "read_sequences" +28 .SPR.command "stats_on" +6 "carousel0"Fh% +3 "core" %(0iPb \  +6 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2922h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Ci% +4 "core" %6P +46 "syringe1"Cj% +4 "core" %6K +46 "syringe1"Ck% +4 "core" %6ɿQ +46 "syringe1"Cl% +4 "core" %6ɽǁ +46 "syringe1"Cm% +5 "core" %6ɶR +45 "syringe1"Cn% +4 "core" %6ɺT +46 "syringe1"Co% +4 "core" %6ɻI +46 "syringe1"Cp% +4 "core" %6ɼL +53 "syringe1"C)% +7 "core" é%6ɽI +37 "syringe1"CT% +3 "core" %6H +47 "syringe1"CN% +3 "core" %6ɾJ +47 "syringe1"CY% +3 "core" %6I +47 "syringe1"C@% +4 "core" %6M +46 "syringe1"CP% +4 "core" %6ɿ +7 "Can"l[y,zRu'Fs +2 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +2 .PinPort.in :locking +2 "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +3 "valve1"EK( N +78 "core" ( +6 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +4 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 800uL +3 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +5 "syringe1"C/(% +7 "syringe0"Bv( +5 ."posLyS2"Lysis2.seek 800uL +9 "syringe0"BH'% +396 "core" ï( +155 ' +271 ( +2 ."PROCESSING"delayUntil "22:23:38.80" +1245 "Can"j[,zQuDq K +11968 k\\+zMuA?i +11972 l\\E+zTu>v +11974 m\\T+z]uBO +11973 l\\o+zFu?7 +9144 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 Br% +3 "core" %%n= ` +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C(& +4 "core" è&%n< V +54 ."Lysis2mixer"Lysis2.seek 550uL +4 ."PROCESSING"Lysis1.seek 550uL +5 "syringe0"Bw' +4 "syringe1"Cx( +2742 "Can"i\\+zIsa +745 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +131 #SPR stabilized +2 "syringe1"Cy& +3 "core" &X^ +4 "syringe0"Bz% +3 "core" %Y] +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +266 #Waiting up to 11:00 for SPR to enter state 1 +8257 "Can"l\\e+zTu1 +3511 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B{% +4 "core" %YK +6 "syringe1"C|& +4 "core" &XR +48 ."Lysis2mixer"Lysis2.seek 250uL +4 ."PROCESSING"Lysis1.seek 250uL +3 "syringe0"B}' +7 "syringe1"C~( +4178 "core" ' +5 ( +3 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +340 #Waiting up to 1:00:00 for SPR to enter state 7 +2162 "Can"m\\+zJt/ +11973 k\\C+zRu- ; +11979 l\\+zOu2MY +11977 k\\+zCu1w +11977 l]+zGu3U +11976 k\\+zCt-5 +11974 l\\+zCu2& +11975 k\\+zIu4{" +11470 #"SPRlogger"SPRsummary:5.5RIU,283RIU,274RIU,395RIU after 1924s +505 "Can"k\\o+zDu$% +11974 l\\O+z:u$K)+ +11969 k\\+zMu$.I +11973 m\\+z@u12(g +11971 m\\+zAu $5 +11971 j\\\\+zFu$w:% +11971 l\\D+zAu0= +11971 k\\+z<` F 22 +3 FU% +3 "core" %(.XPL \  +4 ."detached from tina"Carousel.home.seek 42 +2 "carousel0"FF'( +15 "core" ' +3 "carousel0"F[% +5 "core" %(%4S 2 +2 "syringe1"C& +5 "core" Æ&ށ2 +3 ."detached from tina"Plunge.seek :locked +2 "valve0"D*'Z +3 "core" Ī' +4 "syringe0"B,& +4 "core" ¬&){  +5 ."Archive"Archive.in :unlocking +3 "syringe1"CC% + "syringe0"B? PZ-2 +8 ."Archive"Archive.seek :clear +2 "syringe0"Bt(j +2 "core" %6 +7 ."Air"Air.in :unlocking +2 "syringe1"C]& +5 C> P]z-2 +3 "core" &ށ2Ł +3 ."Air"Air.seek :clear +6 ."Lysis1"Lysis1.in :unlocking +2 "syringe0"B % +2 "syringe1"C' +5 C? P[(2 +7 "core" ‹%ށ3Á ~ +3 ."Lysis1"Lysis1.seek :clear +6 ."Lysis2"Lysis2.in :unlocking +4 "syringe0"B> P\\k(2 +3 "syringe1"C( +4 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B5'j +61 "Can"h\\+zSseJ +2 "core" Ä' +331 ( +42 Þ( +5 µ' +4 "sampler0"H.% +4 "core" Ȯ%% +11 ."detached from tina"Gate.power :motor,:OFF + .Gate.power :analytic1,:OFF +2 .Gate.power :core,:OFF + .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785382321.65EDT @EDT1785382322.27 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +315 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +3 D' +3 "core" ħ +62 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +3 "carousel0"F#<` F 22 +8 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +3 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default + "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D7% +4 D& +7 "core" ķ%F$O(  +2 "carousel0"F% +6 ."Plunge_home"Plunge.home.jog 0 +11 "syringe0"B& +3 "syringe1"C% +4 C%& +3 "valve0"D) +6 "syringe0"Bs% +3 "valve1"EM& +25 "core" ĝ&O,  + Ɯ%N8Q P_ + ›&z  Ú%݋" + å&UA _ + %ٷD  + Ĉ) &*N , +5 ."Air_home"Air.home.jog 0 +12 "valve0"D8% + ."Lysis2_home"Lysis2.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +4 ."PinPort_home"PinPort.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 "syringe1"C) +3 "syringe0"B) +2 B6* +2 "valve0"DI* +6 "syringe1"C* +15 "valve1"E&* +3 "carousel0"F) +21 "core" ĸ%DFO+ + ×) + ) + ¶* + * + Î* + Ŧ* +7 "valve1"EV& +4 "syringe0"Bu% +6 B& +5 "syringe1"CD% +4 ."Plunge_home"Plunge.in :homing +4 "syringe1"C & +3 "valve0"D& +2 D> (2 +15 ."Plunge_home"Plunge.jog 1494 +3 "valve0"D0) +2 "core" ƅ) + &!O  + %r٘{  + Ÿ&c +2 %݁  + Ì&UA + ć&O +8 ."Air_home"Air.in :homing +12 ."Lysis2_home"Lysis2.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Lysis1_home"Lysis1.in :homing +4 ."Toroid_home"Toroid.in :homing + "syringe1"C> -2 +3 ."Archive_home"Archive.in :homing +5 "syringe0"B> (2 + "syringe1"C? (2 +3 "valve1"E?2 +3 "valve0"D?2 +2 ."Air_home"Air.jog 1433 +8 "syringe0"B? -2 +8 ."Lysis2_home"Lysis2.jog 1621 +4 "syringe1"C) +4 ."PinPort_home"PinPort.jog 1242 +7 ."Lysis1_home"Lysis1.jog 1601 +6 ."Toroid_home"Toroid.jog 1363 +3 "syringe0"B)U +4 ."Archive_home"Archive.jog 307 +10 "valve1"E* +2 "valve0"D:*S +5 "syringe1"CE*A +4 "syringe0"B1*3 +13 "core" İ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D' +23 "core" Ē' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +9 "core" ±* +2 ."Archive_home"Archive.setRawPosition! 50 + "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"B( +9 "core" Ã) +3 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +4 ."Air_home"Air.seek :home +2 "syringe1"C' +6 "core" Õ' + “( +3 ."Archive_home"Archive.in :default +2 ."Air_home"Air.in :default +3 "syringe0"B?d PZ<2 + "syringe1"C>d P]z<2 +9 "core" –) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +4 ."Lysis2_home"Lysis2.seek :home +2 "syringe0"BB' +5 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 ."Lysis1_home"Lysis1.seek :home +2 "syringe1"C( +9 "core" ' +3 ."Lysis2_home"Lysis2.in :default + "syringe0"B>d P\\k<2 +10 "core" Ï( +3 ."Lysis1_home"Lysis1.in :default + "syringe1"C?d P[<2 +4 "core" ř* +3 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +5 ."PinPort_home"PinPort.seek :home +3 "valve1"E( +264 "core" ĺ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +43 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +11 "valve0"DJ( +3 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +100 "core" ő( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +4 "valve1"E$& +4 "core" Ť&O +3 "valve0"DG( +8 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E<( +41 "core" ż( +299 ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C!' +5 "core" á' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C ( +67 "core" à( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"BZ'j +74 "core" ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"B2(j +74 "core" ²( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +5 F9% +3 "core" ƹ%(V:KP  +4 ."detached from tina"Carousel.home.seek 42 +3 "carousel0"F+'( +14 "core" ƫ' +2 "valve0"D;% +5 "core" Ļ%^nO2 ! +3 #"detached from tina"Selecting Cartridge 36 +2 "valve0"D3% +4 "core" ij%^d O/ ! +3 "syringe1"C\\& +4 "core" &Qn +3 "syringe1"CO% +4 "core" %݈o +3 "valve1"Eq& +4 "core" & O. @ +3 ."detached from tina"Carousel.seek 36 +2 "carousel0"F#'"2 +145 "core" ƣ' +5 "carousel0"F^% +3 "core" %"2)?S 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"C=& +3 "core" ý&Kn +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CA( +3 "core" ƣ' "6M!P  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 36 + 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiP:@pastThresholdF: @voltagef15.024088365243005ݙ: @currentiR: @type2F:@outPressi!:\ @lostF: @inPressi:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni6": @pastRLSF: @tagi(: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6 ƣ' +242 ( +3 "valve0"D`% +8 "core" %^vO ! +2 ."Plunge"Plunge.seek :locked +3 "valve0"D4'Z +4 "syringe1"C?% +11 "core" ÿ%ݍcw +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"Ca'6 +79 "core" Ĵ' +24 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 Fb% +3 "core" %"2UkQ  +7 #"detached from tina"Powering cartridge bus +268 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" cy +3 "cartridge" y v8 v8 +4 "Cartridge" dy  +5 "cartridge" y 5/J59925 Ǜ/ZCi +3 #"detached from tina"Mounted lysis Cartridge board#J59925 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"De% +3 "core" %ZGO/ [ +3 "syringe1"Cf& +5 "core" &c +2 "syringe1"Cg% +3 "core" %6\ j H +5 "carousel0"Fh% +4 "core" %"2kPR \ +4 "carousel0"Fi% +6 "core" %"2k~PR \ +117 ."PROCESSING"SPR.command "read_sequences" +78 .SPR.command "stats_on" +7 "carousel0"Fj% +3 "core" %"2lPh \ \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul2923h.spr.bz2 +198 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +2 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Ck% +3 "core" %6\ f H +48 "syringe1"Cl% +3 "core" %6 a I +47 "syringe1"Cm% +3 "core" %6c Q +51 "syringe1"Cn% +3 "core" %6 ` F +43 "syringe1"Co% +3 "core" %6^ R +47 "syringe1"Cp% +3 "core" %6a Y +47 "syringe1"C)% +3 "core" é%6d S +47 "syringe1"CT% +3 "core" %6` B +48 "syringe1"CN% +3 "core" %6 L +47 "syringe1"CY% +3 "core" %6\\ Q +47 "syringe1"C@% +3 "core" %6] N +47 "syringe1"CP% +3 "core" %6` Y +47 "syringe1"C>% +3 "core" þ%6Z O +47 "syringe1"C-% +4 "core" í%6_ K +6 "Can"h\\+zSu1q +2 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +3 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"EW( N +79 "core" ( +4 ."posArchive"Archive.in :fast +6 ."posLyS2"Lysis2.in :fast +8 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +3 ."PROCESSING"Lysis1.seek 800uL +10 "syringe1"CL(% +5 ."posLyS2"Lysis2.seek 800uL +6 ."posArchive"Archive.seek :empty +4 "syringe0"Br'% + B(( +398 "core" ( +153 ' +280 ¨( +2 ."PROCESSING"delayUntil "23:42:40.46" +5949 "Can"m[+zSu?| +11973 h[f+zRu@a" +11973 l[2+zUuAV7 +11976 k[P+zVu=U +11974 l[+zRu@Cs +4423 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 BH% +3 "core" %%npG p +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C/& +3 "core" ï&%n L k +55 ."Lysis2mixer"Lysis2.seek 550uL +5 "syringe0"By' +4 ."PROCESSING"Lysis1.seek 550uL +5 "syringe1"Cz( +3482 "core" ' +7 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +132 #SPR stabilized +2 "syringe1"C{& +6 "core" &Xq +5 "syringe0"B|% +4 "core" %Xp +3 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +260 #Waiting up to 11:00 for SPR to enter state 1 +1004 "Can"k[+zTu6\\ +10764 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"Bw% +4 "core" %X́ +5 "syringe1"Cx& +4 "core" &Xz +47 ."Lysis2mixer"Lysis2.seek 250uL +5 ."PROCESSING"Lysis1.seek 250uL +2 "syringe0"B' +6 "syringe1"C( +942 "Can"h[+zTs:t +3237 "core" ' +4 Á( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +344 #Waiting up to 1:00:00 for SPR to enter state 7 +6881 "Can"j[+zQu30 +11969 j[+zRu5 +11976 k[k+zSu28 +11974 l[U+zRu4' +11976 k[r+zTu38E +11974 l\\+zRu3c +11973 j]x+zQu5] @EDT1785384113.58 k]+zSu2 +6767 #"SPRlogger"SPRsummary:4.59RIU,214RIU,209RIU,310RIU after 1924s +5205 "Can"l]$+zSu$E­ +11972 l\\+z>u# +11975 k]5+zQu$!% +11972 k\\+zHu08 +11971 l\\+zGu$5 +11973 j\\#+zPu4S +11970 j\\+zCu1ڣq +8650 "syringe1"C& +4 "core" Â&?TY +3 "syringe0"B\ % +6 "core" Š%?r +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +307 #Waiting up to 11:00 for SPR to enter state 1 +995 "Can"k\\s+zQu4" +10774 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B}% +3 "core" %? +4 "syringe1"C~& +5 "core" &?T +58 ."Lysis2mixer"Lysis2.seek 100uL +4 "syringe0"B 'ށ +4 ."PROCESSING"Lysis1.seek 100uL +5 "syringe1"CQ(ށ +920 "Can"j\\+zSs/tTz +1169 "core" ' +8 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +340 #Waiting up to 1:00:00 for SPR to enter state 7 +8950 "Can"k\\+zPu1& +11973 k\\+zQu5 +11974 m\\+zPu4 + +11975 j\\+zOu.]% +11971 m\\}+zTu3-C +11971 j\\+zNu5a +11972 k\\+zOu2V +11973 j\\+zQu2 +11971 m\\+zQu$= +11972 k]+zQu$t  +11970 j^+zLu$ F +11970 k^+zLu/ +11968 l^+zPu$K3 +11969 k\\+zPu1uQ +11969 k[+zSu1o +6606 #"PROCESSING"SPR analysis completed +54 #"SPRlogger"Ended log spr26jul2923h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +121 "carousel0"FX% +3 "core" %"2uPX \  +7 "sampler0"HU% +4 "core" %V +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +49 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"E[& +3 "core" & N:O  +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" ņ( +4 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F,% +3 "core" Ƭ%"2jPQ \ +4 ."detached from tina"Carousel.home.seek 36 +2 "carousel0"FC'"2 +16 "core" ' +2 "carousel0"F]% +5 "core" %"22lT 2\ +2 "syringe1"C & +4 "core" Ë&ށ2Ձ +4 ."detached from tina"Plunge.seek :locked +2 "valve0"DK'Z +3 "core" ' +3 "syringe0"BS& +5 "core" &)  +4 ."Archive"Archive.in :unlocking +3 "syringe1"C"% +5 "syringe0"B? PZ-2 +5 "core" â%6 { +2 ."Archive"Archive.seek :clear +5 "syringe0"Bv(j +2 ."Air"Air.in :unlocking +11 "syringe1"C_& +5 C> P]z-2 +5 "core" &ށ2 +3 ."Air"Air.seek :clear +5 ."Lysis1"Lysis1.in :unlocking +4 "syringe1"C' +3 "syringe0"B% +8 "syringe1"C? P[(2 +5 ."Lysis1"Lysis1.seek :clear +2 "syringe1"C.( +4 "core" ”%ށ3o܁9 +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B''j +58 "core" Ø' +336 ( +30 î( +17 §' +4 "sampler0"H7% +4 "core" ȷ% +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +2 .Gate.power :core,:OFF + .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785387062.47EDT @EDT1785387062.67 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +357 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +32 "valve0"DP +60 DM +3 "core"  +11 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +6 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +8 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default + "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default + "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +5 "valve0"Ds% +3 D%& +5 "carousel0"F% +5 "syringe0"B& +5 "syringe1"C% +7 C& +3 "syringe0"B8% +5 "valve1"EV& +4 "core" %FO+  +7 ."Plunge_home"Plunge.home.jog 0 +21 "valve0"Du) +15 "core" ĥ&O* + ƚ%NsQ j$ + ›&z  + Ü%݃( + Ý&T ` + ¸%4  &O$ -h + ) +9 "valve0"D % +7 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +2 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +3 ."Air_home"Air.home.jog 0 +3 "valve1"ED* +3 "syringe1"C* +7 "syringe0"B* +8 "valve0"D** +5 "syringe0"B) +5 "carousel0"F5) +5 "syringe1"Ct) +18 "core" Č%GFO( + * + Ç* Ÿ* + Ī* + „) + ) Ƶ) +5 "valve1"ER& +7 "syringe1"C & +5 "syringe0"B% +10 "syringe1"CF% +2 "valve0"D& +9 "syringe0"B& +3 ."Plunge_home"Plunge.in :homing +4 "valve0"D> (2 +12 ."Plunge_home"Plunge.jog 1493 +2 "valve0"D) +7 "core" &*6O +2 É&Hh  + ž%nٌh + %݆x + —&di + Ĉ&O +12 ."PinPort_home"PinPort.in :homing +3 ."Lysis2_home"Lysis2.in :homing +2 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +3 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 "valve1"E?2 +3 "syringe0"B> (2 +2 "syringe1"C> -2 +5 "valve0"D?2 +2 "syringe1"C? (2 +6 "syringe0"B? -2 +6 ."Lysis2_home"Lysis2.jog 1622 +7 ."PinPort_home"PinPort.jog 1250 +3 ."Air_home"Air.jog 1432 +4 ."Toroid_home"Toroid.jog 1364 +3 ."Archive_home"Archive.jog 306 +4 ."Lysis1_home"Lysis1.jog 1604 +3 "syringe0"B&)V +3 "valve1"E* +3 "syringe1"CI) +2 "valve0"D6*T +6 "syringe0"B0*2 +2 "syringe1"C1*D +26 "core" Đ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D' +23 "core" °* +2 ă' +2 ."Archive_home"Archive.setRawPosition! 50 +3 ."Plunge_home"Plunge.in :default + "syringe0"B82 +2 "valve0"D> P ޤ-2 +8 ."Archive_home"Archive.seek :home +2 "syringe0"B( +25 "core" –( +4 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +7 "core" ) +2 ¦) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 + ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +2 "syringe0"B72 +9 ."Lysis2_home"Lysis2.seek :home +2 ."Air_home"Air.seek :home +6 "syringe0"BE' + "syringe1"C' +10 "core" ñ* + Ù' +3 ."Air_home"Air.in :default + ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe1"C82 +2 C>d P]z<2 +7 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C$( +8 "core" ' + Ŏ* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Lysis2_home"Lysis2.in :default + "valve1"E8 N +3 "syringe0"B>d P\\k<2 +4 ."PinPort_home"PinPort.seek :home +5 "valve1"E:( +5 "core" ä( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +242 "core" Ķ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +3 "valve0"DJ( +5 "core" ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +162 "core" ź( +2 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +3 "valve1"E& +4 "core" ő&O % +2 "valve0"D9( +7 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E;( +41 "core" Ż( +299 Ĺ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C3' +4 "core" ó' +3 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C\\( +67 "core" ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"BO'j +74 "core" ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"Bq(j +73 "core" ( +3 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 F% +4 "core" ƕ%"1YwbQ  +4 ."detached from tina"Carousel.home.seek 36 +2 "carousel0"F'"2 +14 "core" Ə' +3 "valve0"DB% +3 "core" %^O1 !} +4 #"detached from tina"Selecting Cartridge 30 +3 "valve0"D% +3 "core" ē%^O3 !z +3 "syringe1"C^& +4 "core" &KX +3 "syringe1"C=% +5 "core" ý%ݎ_ +2 "valve1"E& +4 "core" Ņ&yO6  +4 ."detached from tina"Carousel.seek 30 +2 "carousel0"F+'X +145 "core" ƫ' +4 "carousel0"F`% +6 "core" %X1}zS 2 +4 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +5 "syringe1"C?& +3 "core" ÿ&L[ +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C ( +3 "core" ƫ' \\V} Q  +3 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 30 + 4 counts (sensed 30 + 5 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.019179185076093T: @currenti[: @type2F:@outPressi :\ @lostF: @inPressi}:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni\\: @pastRLSF: @tagi0: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +8 ƫ' +242 à( +2 "valve0"Db% +6 "core" %^}OO ! +4 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +5 "syringe1"C!% +7 "core" á%ݍ\\ +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +5 ."Air"Air.seek :locked +2 "syringe1"Cc'6 +84 "core" Ē' +18 ' +3 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 Fd% +2 "core" %X]}aQ  +8 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" ey +3 "cartridge" y RI +4 "Cartridge" fy  +4 "cartridge" y k`/ C;R c/J59986 +4 #"detached from tina"Mounted lysis Cartridge board#J59986 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"Dg% +3 "core" %ZGO1 Z +4 "syringe1"Ch& +3 "core" &T +3 "syringe1"Ci% +3 "core" %6 > +3 "carousel0"Fj% +4 "core" %X}Pg \ +4 "carousel0"Fk% +6 "core" %X}Pq \ +122 ."PROCESSING"SPR.command "read_sequences" +58 .SPR.command "stats_on" +6 "carousel0"Fl% +4 "core" %X}P[ \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3000h.spr.bz2 +251 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cm% +5 "core" %6M B +46 "syringe1"Cn% +3 "core" %6L @ +55 "syringe1"Co% +3 "core" %6M 8 +39 "syringe1"Cp% +6 "core" %6M > +44 "syringe1"C)% +3 "core" é%6M = +47 "syringe1"CT% +3 "core" %6M D +47 "syringe1"CN% +3 "core" %6V : +47 "syringe1"CY% +3 "core" %6P : +7 "Can"j[-+zSuD  +41 "syringe1"C@% +3 "core" %6S = +47 "syringe1"CP% +3 "core" %6!N 1 +47 "syringe1"C>% +3 "core" þ%6 M 2 +47 "syringe1"C-% +3 "core" í%6 L 2 +49 "syringe1"C<% +5 "core" ü%6F 9 +43 "syringe1"CA% +5 "core" %6M 4 +45 "syringe1"CZ% +3 "core" %6 = +47 "syringe1"C2% +3 "core" ò%6D 3 +47 "syringe1"CH% +3 "core" %6J < +47 "syringe1"C/% +3 "core" ï%6K > +47 "syringe1"Cr% +3 "core" %6S @ +47 "syringe1"CL% +3 "core" %6L = +47 "syringe1"C{% +3 "core" %6 I F +47 "syringe1"C|% +3 "core" %6K > +47 "syringe1"Cw% +3 "core" %6I 8 +47 "syringe1"Cx% +4 "core" %6K C +46 "syringe1"Cy% +3 "core" %6N 7 +47 "syringe1"Cz% +3 "core" %6E ; +47 "syringe1"C% +3 "core" Â%6I 7 +47 "syringe1"C\ % +3 "core" Ê%6B 7 +47 "syringe1"C}% +3 "core" %6B : +47 "syringe1"C~% +6 "core" %6E B +44 "syringe1"C% +3 "core" %6A @ +48 "syringe1"C% +3 "core" Á%6C < +46 "syringe1"CX% +3 "core" %6  E +47 "syringe1"CU% +4 "core" %6D 6 +4 "Can"j[-+zSu,!@ +2 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +2 .PinPort.in :locking +2 "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"E[( N +78 "core" ( +4 ."posArchive"Archive.in :fast +7 ."posLyS2"Lysis2.in :fast +3 "syringe0"B?d PZ<2 +2 B>d P\\k<2 +2 ."PROCESSING"Lysis1.seek 800uL +8 "syringe1"C,(% +7 ."posLyS2"Lysis2.seek 800uL +6 ."posArchive"Archive.seek :empty +4 "syringe0"B]'% +4 B ( +395 "core" ì( +153 ' +283 ‹( +3 ."PROCESSING"delayUntil "01:01:42.20" +9703 "Can"l[+zUuB$ +11971 l\\-+zQuAu' +11971 l]+zTu@W* +11972 l^+zSuD@.' +11701 ."PROCESSING"Lysis2.in :DAmix +9 "syringe0"B>d2 P\\k<2 +6 BS% +3 "core" %%nq, o +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +4 C"& +3 "core" â&%n0 a +44 ."Lysis2mixer"Lysis2.seek 550uL +4 ."PROCESSING"Lysis1.seek 550uL +4 "syringe0"B_' +3 "syringe1"C( +184 "Can"j^+zRs\\1w# +3306 "core" ' +3 Ô( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +129 #SPR stabilized + "syringe1"CW& +3 "core" &X +5 "syringe0"B#% +5 "core" £%Y +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +265 #Waiting up to 11:00 for SPR to enter state 1 +5693 "Can"j]+zPu37A +6075 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B4% +3 "core" ´%Y\ N +4 "syringe1"C(& +6 "core" è&XO +54 ."Lysis2mixer"Lysis2.seek 250uL +4 ."PROCESSING"Lysis1.seek 250uL +4 "syringe0"Ba' +3 "syringe1"CQ( +4182 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1491 "Can"l]O+zRt/ >_ +11 ."PROCESSING"SPR.command "hold_off" +293 #Waiting up to 1:00:00 for SPR to enter state 7 +11668 "Can"k\\+zSu2B} +11973 l\\K+zSu1BF +11973 l[+zRu2J +11971 j[+zNu5M +11974 k[2+zTu1Q  +11972 l[+zSu1'T +11970 l[d+zHu4 X71 +11973 k[+zPu6[O +2085 #"SPRlogger"SPRsummary:5.31RIU,155RIU,144RIU,219RIU after 1925s +9882 "Can"k[+zSu$`_m +11969 i[+zTu$Zd +11971 l[+zQu$qhW +11972 k[+zSu$k +11969 j[+zSu$p5 +11970 k[+zOu1t +11974 l[2+zSu3w! +3983 "syringe1"C & +3 "core" Í&?S( +4 "syringe0"B7% +5 "core" ·%?{ +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +314 #Waiting up to 11:00 for SPR to enter state 1 +5661 "Can"k[ +zPu5{? +6102 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"BM% +4 "core" %?I +3 "syringe1"Cs& +7 "core" &?\\ +57 ."Lysis2mixer"Lysis2.seek 100uL +5 ."PROCESSING"Lysis1.seek 100uL +2 "syringe0"BV'ށ +6 "syringe1"C8(ށ +2089 "core" ' +5 ø( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +301 #Waiting up to 1:00:00 for SPR to enter state 7 +1749 "Can"m[+zet/ ] +11971 l\\+zQu0#{ +11972 n]+zlu5 +11976 l]:+zMu0- +11972 m\\+zUu5 +11972 l\\5+zTu5&4 +11972 k[+zTu4 +11973 k[v+zPu3c/ +11972 l[p+zTu$M +11974 l[P+zWu$H)k +11971 k[3+zUu$j +11970 k[@+zSu1v +11972 k[b+z[u$E +11971 k\\+zQu$[ +11970 j\\+zTu/D +11971 k\\+zdu3 +1921 #"PROCESSING"SPR analysis completed +54 #"SPRlogger"Ended log spr26jul3000h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +121 "carousel0"F% +4 "core" Ɲ%X}P \ +6 "sampler0"H% +3 "core" Ȝ%) +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E& +3 "core" ś& N9O+  +4 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +7 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" Ś( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F%% +2 "core" ƥ%X}[Pe \  +4 ."detached from tina"Carousel.home.seek 30 +3 "carousel0"F 'X +15 "core" ƌ' +2 "carousel0"FR% +4 "core" %X3}T 2 +2 "syringe1"C& +6 "core" Þ&ށ2Ђ +2 ."detached from tina"Plunge.seek :locked +3 "valve0"D 'Z +4 "core" ĉ' +3 "syringe0"BF& +4 "core" &)|W  +5 ."Archive"Archive.in :unlocking +4 "syringe1"C% + "syringe0"B? PZ-2 +8 "core" ×%6X \\ +2 ."Archive"Archive.seek :clear +5 ."Air"Air.in :unlocking +6 "syringe1"CK& + "syringe0"B(j +2 "syringe1"C> P]z-2 +10 ."Air"Air.seek :clear +5 "syringe1"C' +6 "core" &ށ2W +8 ."Lysis1"Lysis1.in :unlocking +2 "syringe0"B.% + "syringe1"C? P[(2 +9 ."Lysis1"Lysis1.seek :clear +2 "syringe1"Cv( +2 "core" ®%ށ3r +7 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"BG'j +48 "core" Æ' +355 ˆ( +22 ( +16 ' +3 "sampler0"H'% +4 "core" ȧ%V +11 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785391804.20EDT @EDT1785391804.40 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +357 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 DC +3 "core"  +67 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default + "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default + "carousel1"G$ +7 ."detached from tina"Archive.configure :default + "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default + "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +3 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D% +3 Du& +6 "core" Ę%FO#  + "carousel0"F% +7 "syringe0"BD& +8 "syringe1"Ct% +11 C*& +3 ."Plunge_home"Plunge.home.jog 0 +16 "syringe0"B% +2 "valve1"E& +3 "valve0"D) +15 "core" &gO& 5 + Ƅ%N}Q  +2 &h"  +2 %݇h +2 ê&<\ x +2 Ÿ%وj  Ň&/O# - + Đ) +9 "valve0"D0% +7 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 "valve1"E&* +3 "syringe1"CI* +4 "syringe0"B1* +9 "valve0"D* +4 "syringe0"B) +4 "syringe1"C6) +5 "carousel0"FJ) +20 "core" İ%HFO& \ + Ŧ* + * ±* + Ď* + ‘) ö) + ) +8 "valve1"E:& +3 "syringe0"B% +5 "valve0"D& +2 "syringe1"CB% +2 C^& +4 ."Plunge_home"Plunge.in :homing +5 "syringe0"B=& +4 "valve0"D> (2 +24 ."Plunge_home"Plunge.jog 1492 +2 "valve0"D) +5 "core" ź&3O + •%oًh  + ē&eO  %݆f + &7  + ½&b  +11 ."PinPort_home"PinPort.in :homing +4 ."Lysis2_home"Lysis2.in :homing +2 ."Archive_home"Archive.in :homing +2 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 "valve1"E?2 + "syringe0"B> (2 +3 B? -2 +5 "syringe1"C> -2 +12 "valve0"D?2 +5 "syringe1"C? (2 +2 ."Lysis2_home"Lysis2.jog 1623 +4 ."PinPort_home"PinPort.jog 1258 +6 ."Archive_home"Archive.jog 308 +4 ."Air_home"Air.jog 1432 +8 "syringe0"B)W +2 "valve1"E$* +3 "syringe0"BE*4 +2 ."Toroid_home"Toroid.jog 1357 +5 "syringe1"C`) +2 ."Lysis1_home"Lysis1.jog 275 +10 "valve0"D*M +5 "syringe1"C?* +24 "core" ą) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D5' +13 "core" * +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +3 "syringe0"B( +6 "core" ĵ' ÿ* +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 ."Plunge_home"Plunge.in :default +2 "syringe1"C82 + "valve0"D> P ޤ-2 +8 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"Cb( +6 "core" ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +5 "core" ™) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +4 "core" ( +4 ."Lysis1_home"Lysis1.in :default +4 ."Lysis2_home"Lysis2.seek :home +2 "syringe1"C?d P[<2 +3 "syringe0"B!' +11 "core" ) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +4 ."Air_home"Air.seek :home +2 "syringe1"C\\' +7 "core" ' + ¡' +3 ."Air_home"Air.in :default +2 ."Lysis2_home"Lysis2.in :default +2 "syringe1"C>d P]z<2 + "syringe0"B>d P\\k<2 +9 "core" Ť* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +4 ."PinPort_home"PinPort.seek :home +2 "valve1"Ed( +271 "core" Ė* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +48 ."Toroid_home"Toroid.seek :home +12 "valve0"D( +3 "core" ă( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +92 "core" ( +2 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +4 "valve1"E3& +2 "valve0"De( +5 "core" ų&6O +7 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"Ef( +41 "core" ( +299 ( +4 ."detached from tina"Air.seek :clear +3 "syringe1"Cg' +4 "core" ' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"Ch( +68 "core" ( +2 ."detached from tina"Lysis2.seek :clear +3 "syringe0"Bi'j +73 "core" ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"Bj(j +74 "core" ( +2 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 Fk% +3 "core" %VQ}AP  +5 ."detached from tina"Carousel.home.seek 30 +2 "carousel0"Fl'X +15 "core" ' +2 "valve0"Dm% +4 "core" %^rO. ! +4 #"detached from tina"Selecting Cartridge 24 + "valve0"Dn% +5 "core" %^|O0 ! +3 "syringe1"Co& +4 "core" &J] +3 "syringe1"Cp% +4 "core" %ݎ7 +3 "valve1"E)& +4 "core" ũ&O/  +3 ."detached from tina"Carousel.seek 24 +4 "carousel0"FT' +145 "core" ' +5 "carousel0"FN% +3 "core" %3dgT 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"CY& +3 "core" &B\\ +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"C@( +247 "core" ( +2 "valve0"DP% +7 "core" %^O !y +3 ."Plunge"Plunge.seek :locked +2 "valve0"D>'Z +5 "syringe1"C-% +6 "core" í%ݎ[ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C<'6 +84 "core" ľ' +19 ü' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +9 FA% +4 "core" %]dRQD  +7 #"detached from tina"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" Zy +3 "cartridge" yI͡BI͡B +4 "Cartridge" 2y  +5 "cartridge" y $/J59995 "/6CG +3 #"detached from tina"Mounted lysis Cartridge board#J59995 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"DH% +3 "core" %ZG\\O+ U +3 "syringe1"C/& +5 "core" ï&X +2 "syringe1"Cr% +5 "core" %6 V I +2 "carousel0"FL% +5 "core" %dRPj \ +3 "carousel0"F{% +7 "core" %dPPx \ +117 ."PROCESSING"SPR.command "read_sequences" +6 .SPR.command "stats_on" +81 "carousel0"F|% +4 "core" %dQPS \ \ +5 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26jul3002h.spr.bz2 +205 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +2 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cw% +3 "core" %6M M +48 "syringe1"Cx% +3 "core" %6 L I +47 "syringe1"Cy% +3 "core" %6 O G +47 "syringe1"Cz% +3 "core" %6M @ +54 "syringe1"C% +10 "core" Â%6N K +33 "syringe1"C\ % +3 "core" Ê%6 O E +47 "syringe1"C}% +3 "core" %6W M +47 "syringe1"C~% +3 "core" %6N J +47 "syringe1"C% +4 "core" %6 L I +46 "syringe1"C% +4 "core" Á%6 N G +46 "syringe1"CX% +4 "core" %6 M F +46 "syringe1"CU% +4 "core" %6Q N +46 "syringe1"C;% +4 "core" û%6L L +46 "syringe1"C % +4 "core" à%6L M +46 "syringe1"CO% +4 "core" %6G M +47 "syringe1"Cq% +5 "core" %6\ I H +44 "syringe1"CS% +4 "core" %6\ J H +46 "syringe1"C"% +4 "core" â%6\ K H +47 "syringe1"C]% +3 "core" %6 K G +46 "syringe1"C,% +4 "core" ì%6 I G +46 "syringe1"CW% +4 "core" %6H Q +46 "syringe1"C#% +4 "core" ã%6K M +46 "syringe1"C4% +4 "core" ô%6J K +46 "syringe1"C(% +4 "core" è%6J L +46 "syringe1"C_% +4 "core" %6 H G +46 "syringe1"C% +4 "core" Ô%6F ? +46 "syringe1"C % +4 "core" Í%6G M +46 "syringe1"C7% +4 "core" ÷%6 D F +46 "syringe1"CM% +4 "core" %6D L +46 "syringe1"Cs% +4 "core" %6G N +46 "syringe1"Ca% +4 "core" %6 M +46 "syringe1"CQ% +4 "core" %6 H\ I +47 "syringe1"C% +6 "core" Ý%6I C +43 "syringe1"C% +3 "core" Ü%6\ J H +47 "syringe1"C% +3 "core" Û%6A K +47 "syringe1"C%% +4 "core" å%6 O +46 "syringe1"CR% +4 "core" %6 N +46 "syringe1"C% +4 "core" Þ%6 J +46 "syringe1"CF% +4 "core" %6  F +46 "syringe1"C% +4 "core" ×%6 S +4 "Can"k\\+zdu$6 + #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"EK( N +78 "core" ( +4 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 800uL +3 ."posArchive"Archive.seek :empty +3 "syringe0"B>d P\\k<2 +4 "syringe1"C.(% +3 "syringe0"B[( +10 ."posLyS2"Lysis2.seek 800uL +7 "syringe0"B+'% +396 "core" î( +154 «' +266 ( +4 ."PROCESSING"delayUntil "02:20:43.02" +2187 "Can"j\\+zTu=V= +11970 l\\+zVuAN[ +11974 l\\y+zTu@y +11971 k\\L+zRu@f +11972 l\\8+zUu?j. +6914 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +4 B% +3 "core" ’%%n X +6 ."PROCESSING"Lysis1.in :DAmix +3 "syringe1"C?d2 P[<2 +5 C & +3 "core" Ë&%n b +45 ."Lysis2mixer"Lysis2.seek 550uL +4 ."PROCESSING"Lysis1.seek 550uL +4 "syringe0"Bc' +2 "syringe1"C8( +3485 "core" ' +4 ø( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +140 #SPR stabilized + "syringe1"CV& +4 "core" &X{ +3 "syringe0"B'% +6 "core" §%Yv +2 ."PROCESSING"delay 20 seconds +775 "Can"i[+zRu83 +1228 ."PROCESSING"SPR.command "start 2 0" +264 #Waiting up to 11:00 for SPR to enter state 1 +10478 "Can"k[+zPu4E +1289 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"BC% +4 "core" %YP +3 "syringe1"C& +6 "core" Ø&Xׁ +51 ."Lysis2mixer"Lysis2.seek 250uL +5 ."PROCESSING"Lysis1.seek 250uL +3 "syringe0"B' +6 "syringe1"C( +4176 "core" ‡' +5 ß( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +353 #Waiting up to 1:00:00 for SPR to enter state 7 +4425 "Can"k[+zTt.}  +11969 m]+zPu3) - +11972 j^+zUu0 K +11971 l^+zTu24 i +11969 j]+zTu5 +11967 l]g+zQu7d +11971 l\\+zSu0 +11970 j\\+zOu5e +9385 #"SPRlogger"SPRsummary:5.4RIU,55.7RIU,42RIU,83.7RIU after 1925s +2588 "Can"l\\+zPu$I +11970 k[+zOu#T! +11972 j[?+zRu$+!; +11969 j[4+zUu0v!Y +11971 k[+zTu$A p!w +11974 k[+zNu$T ! +11973 k[+zMu0G! +11274 "syringe1"C*& +3 "core" ê&?Y +4 "syringe0"Bt% +3 "core" %? +3 ."PROCESSING"delay 20 seconds +688 "Can"m[+zQu;c! +1315 ."PROCESSING"SPR.command "start 2 0" +361 #Waiting up to 11:00 for SPR to enter state 1 +10298 "Can"k[+zKu2L! +1512 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"BD% +4 "core" %?\\ +3 "syringe1"C& +6 "core" Ä&?[ +57 ."Lysis2mixer"Lysis2.seek 100uL +4 ."PROCESSING"Lysis1.seek 100uL +3 "syringe0"Bu'ށ +3 "syringe1"C0(ށ +2096 "core" ' +5 ð( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +339 #Waiting up to 1:00:00 for SPR to enter state 7 +6245 "Can"j[+zSu3" +11973 l[+zRu1"U"+ +11971 k[w+zUu2%"I +11971 j[_+zWu3)^"g +11972 l[5+zOu4C," +11976 l[+zWu/b0" +11977 l[0+zYu/4" +11975 j\\@+zSu07" +11970 l]8+zTu$x;b" +11967 i]+zhu$:?# +11971 k] +z[u$)C#9 +11970 k[+z_u0oG#W +11968 jZ+zTu$UK#u +11968 iZF+zPu0O# +11967 lZ%+zSu59Su# +9318 #"PROCESSING"SPR analysis completed +52 #"SPRlogger"Ended log spr26jul3002h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +115 "carousel0"F:% +3 "core" ƺ%dQP \ \ +7 "sampler0"H% +4 "core" ȕ%H +4 ."closeHandoff"Handoff.select :close +9 "valve0"D +47 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E^& +3 "core" & N:O%  +4 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +3 ."detached from tina"PinPort.seek :clear +2 "valve1"EB( +78 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F=% +3 "core" ƽ%dPTPd \ +4 ."detached from tina"Carousel.home.seek 24 +2 "carousel0"F' +16 "core" Ɠ' +2 "carousel0"F % +5 "core" Ɖ%'dXS 2 +2 "syringe1"C& +5 "core" Ú&ށ2 +4 ."detached from tina"Plunge.seek :locked +5 "valve0"Dv'Z +4 "core" ' +3 "syringe0"B& +4 "core" ˆ&){t  +5 ."Archive"Archive.in :unlocking +3 "syringe1"C9% +4 "syringe0"B? PZ-2 +6 "core" ù%6x a +3 ."Archive"Archive.seek :clear +5 "syringe1"C& +4 ."Air"Air.in :unlocking +5 "syringe0"BG(j +3 "syringe1"C> P]z-2 +5 "core" Æ&ށ2y +4 ."Air"Air.seek :clear +4 "syringe1"C ' +3 ."Lysis1"Lysis1.in :unlocking +7 "syringe0"B% + "syringe1"C? P[(2 +8 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C( +4 "core" %ށ3e +5 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B&'j +55 "core" Ì' +350 ( +19 Ñ( +17 ¦' +3 "sampler0"H1% +5 "core" ȱ%R +10 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785396546.89EDT @EDT1785396547.08 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +359 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +7 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +3 D6 +3 "core" Ķ +6 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +64 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default + "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default + "carousel0"F#<` F 22 +7 ."detached from tina"Abs.configure :default + "ambient0"J#  +7 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default + "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D% +3 DI& +4 "carousel0"F% +5 "syringe0"BE& +11 "syringe1"C?% +5 C& +5 "syringe0"B`% +7 "valve1"E$& +16 "core" Ď%FO'  + & O) Ԑ + ƅ%NdXQ ? + &שp 6 ÿ%݇3 + Ù&@5 t + %ٓ5  +8 ."Lysis1_home"Lysis1.home.jog 0 +18 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Plunge_home"Plunge.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +6 ."Air_home"Air.home.jog 0 +2 ."Toroid_home"Toroid.home.jog 0 +4 "syringe1"C3* +2 ."Archive_home"Archive.home.jog 0 +8 "valve0"D) +3 "syringe0"B) +4 "carousel0"Fd) +5 "syringe1"Ck) +4 "valve0"Dm* +6 "syringe0"Bn* +23 "core" Ť&O( - + ó* + Ė) + ƒ) * + ) + * + ) +5 "syringe0"Bo% +9 "syringe1"Cp& +3 C)% +3 ."PinPort_home"PinPort.home.jog 0 +12 "valve0"D\\% +4 Db& +2 "syringe0"B!& +6 "valve1"E* +16 "core" %qٴp + &B0 + é%݀m  + %IGO + &O + ¡&_׻l ŏ* +8 "valve1"EN& +7 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +5 ."Toroid_home"Toroid.in :homing +2 ."Archive_home"Archive.in :homing +2 ."Plunge_home"Plunge.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 "syringe0"B> (2 + "syringe1"C> -2 +3 "valve0"D?2 +6 "syringe0"B? -2 +5 "valve0"D> (2 +3 "syringe1"C? (2 +3 ."Lysis2_home"Lysis2.jog 1615 +3 ."Air_home"Air.jog 1433 +6 ."Toroid_home"Toroid.jog 1348 +9 "syringe0"BY)O +4 "syringe1"CJ) +3 ."Archive_home"Archive.jog 309 +6 ."Lysis1_home"Lysis1.jog 1604 +4 ."Plunge_home"Plunge.jog 1486 +8 "valve0"Dl*D +2 "syringe0"BP*5 +4 "syringe1"C-*D +2 "valve0"Dh) +19 "core" &.O  +3 ."PinPort_home"PinPort.in :homing +2 "valve1"E?2 +4 ."PinPort_home"PinPort.jog 1254 +2 "valve1"EA* +24 "core" * +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +2 "syringe0"B5( +24 "core" µ( +2 ."Archive_home"Archive.in :default +3 "syringe0"B?d PZ<2 +3 "core" ) +2 ) +2 ."Air_home"Air.setRawPosition! 0 +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe1"C7 + "syringe0"B72 +7 ."Lysis2_home"Lysis2.seek :home +3 ."Air_home"Air.seek :home +3 "syringe0"Bg' +4 "syringe1"CZ' +8 "core" í* + ) + ' +5 ."Plunge_home"Plunge.setRawPosition! 50 +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 ."Air_home"Air.in :default +2 "valve0"D72 +2 "syringe1"C82 +3 C>d P]z<2 +8 ."Plunge_home"Plunge.seek :home +3 ."Lysis1_home"Lysis1.seek :home +3 "valve0"D2' +4 "syringe1"CH( +10 "core" ' +2 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +8 "core" IJ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +3 "core" ( +4 ."Lysis1_home"Lysis1.in :default +3 "syringe1"C?d P[<2 +2 "core" * +4 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +4 ."PinPort_home"PinPort.seek :home +2 "valve1"E/( +219 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"Dr( +50 "core" ( +4 ."Toroid_home"Toroid.in :default +11 "valve0"D? ֜2 +28 "Can"jZ+zUt(WK# +111 "core" ů( +2 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +5 "valve1"EL& +2 "valve0"D{( +5 "core" &O v +5 ."detached from tina"PinPort.in :unlocking +3 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E|( +41 "core" ( +298 ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"Cw' +5 "core" ' +3 ."detached from tina"Lysis1.seek :clear +2 "syringe1"Cx( +68 "core" ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"By'j +73 "core" ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"Bz(j +74 "core" ( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F% +3 "core" Ƃ%UdZ6Q  +4 ."detached from tina"Carousel.home.seek 24 +3 "carousel0"F\ ' +14 "core" Ɗ' +2 "valve0"D}% +5 "core" %^uO0 ! +7 #"detached from tina"Selecting Cartridge 17 +3 "valve0"D~% +10 "core" %^{\ O- ! +4 "syringe1"C& +10 "core" &J\\ +4 "syringe1"C% +10 "core" Á%ݎ` +5 "valve1"EX& +9 "core" &v O-  +6 ."detached from tina"Carousel.seek 17 +4 "carousel0"FU' +167 "core" ' +5 "carousel0"F;% +3 "core" ƻ%'FS 2 +4 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"C & +3 "core" à&IY +4 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +2 "syringe1"CO( +3 "core" ' NF!Q!  +4 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 17 + 5 counts (sensed 17 + 6 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi!Q:@pastThresholdF: @voltagef15.024088365243005ݙ: @currentiS: @type2F:@outPressi!:\ @lostF: @inPressiF:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @tagiZ: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ' +239 ( +3 "valve0"Dq% +7 "core" %^qOE ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"DS'Z +6 "syringe1"C"% +10 "core" â%݋] +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C]'6 +78 "core" ' +25 ' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F,% +3 "core" Ƭ%[FsQ  +7 #"detached from tina"Powering cartridge bus +267 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default + "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" Wy +3 "cartridge" y' ' +4 "Cartridge" #y  +4 "cartridge" y /J59993 /CF +4 #"detached from tina"Mounted lysis Cartridge board#J59993 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D4% +4 "core" Ĵ%ZFO/ b +3 "syringe1"C(& +4 "core" è&T +3 "syringe1"C_% +3 "core" %6'T + +3 "carousel0"F% +4 "core" Ɣ%FsP] \  +4 "carousel0"F % +6 "core" ƍ%FrPh \  +117 ."PROCESSING"SPR.command "read_sequences" +6 .SPR.command "stats_on" +6 "carousel0"F7% +4 "core" Ʒ%FtuPV \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3003h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CM% +3 "core" %6,P & +48 "syringe1"Cs% +3 "core" %6.U $ +47 "syringe1"Ca% +3 "core" %6M 9 +47 "syringe1"CQ% +3 "core" %6/ # +58 "syringe1"C% +5 "core" Ý%6Q 6 +34 "syringe1"C% +3 "core" Ü%6 N 2 +47 "syringe1"C% +3 "core" Û%6N 5 +47 "syringe1"C%% +3 "core" å%6'M + +47 "syringe1"CR% +4 "core" %6 P 2 +46 "syringe1"C% +4 "core" Þ%6"M 0 +47 "syringe1"CF% +3 "core" %6)` ) +46 "syringe1"C% +4 "core" ×%6N 7 +47 "syringe1"Cf% +3 "core" %6N 6 +46 "syringe1"C@% +7 "core" %60M " +6 "Can"jZ+zUu,X[#g +2 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +3 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"Ej( N +79 "core" ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +4 ."PROCESSING"Lysis1.seek 800uL +2 "syringe0"B>d P\\k<2 +3 ."posArchive"Archive.seek :empty +5 "syringe1"Ci(% +5 ."posLyS2"Lysis2.seek 800uL +5 "syringe0"B( +9 B '% +401 "core" ( +150 ‹' +277 ’( +2 ."PROCESSING"delayUntil "03:39:45.71" +8046 "Can"l\\%+zWu<[i# +11972 k]*+zOu>|^$ +11973 k]+zRu@7a$) +11973 m]+zRuAOe $G +11975 k]+zXu>h@$e +2333 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 B+% +3 "core" «%%n T +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +4 C.& +3 "core" î&%n X +53 ."Lysis2mixer"Lysis2.seek 550uL +6 ."PROCESSING"Lysis1.seek 550uL +2 "syringe0"BV' +3 "syringe1"C'( +3485 "core" ' +4 ç( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +135 #SPR stabilized +3 "syringe1"CC& +3 "core" &X +4 "syringe0"B% +4 "core" ˜%Y +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +268 #Waiting up to 11:00 for SPR to enter state 1 +3094 "Can"l\\+zeu1m$ +8670 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"Bc% +4 "core" %Y\ Z +3 "syringe1"C8& +6 "core" ø&XӁ +52 ."Lysis2mixer"Lysis2.seek 250uL +4 "syringe0"B*' +2 ."PROCESSING"Lysis1.seek 250uL +7 "syringe1"Ct( +3032 "Can"j\\+zSsvt $ +1145 "core" ª' +6 ( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +392 #Waiting up to 1:00:00 for SPR to enter state 7 +8923 "Can"k[+zSu0`y$ +11971 k[+zPu3g}]$ +11972 m[+zRu2J$ +11965 k[+zTu1g% +11967 l[p+zSu3B%7 +11966 k[+zSu0<%U +11970 m\\4+zSu0#%s +11970 j\\+zQu0% +4801 #"SPRlogger"SPRsummary:5.94RIU,24.8RIU,16.1RIU,38.5RIU after 1925s +7168 "Can"m\\+zTu$(% +11972 k\\+zVu$,% +11972 l\\+zcu$A% +11972 i\\+zTu/& +11974 k\\+zVu$}&' +11971 l\\+zWu/&E +11970 l\\f+zRu2&c +6691 "syringe1"CD& +4 "core" &?b~ +2 "syringe0"B% +7 "core" „%? +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +2955 "Can"j\\8+zVu.& +8813 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"B% +4 "core" ‡%?M +3 "syringe1"C& +6 "core" ß&?_S +60 ."Lysis2mixer"Lysis2.seek 100uL +6 ."PROCESSING"Lysis1.seek 100uL +3 "syringe0"B:'ށ + "syringe1"C(ށ +2096 "core" º' +3 Õ( +2 ."PROCESSING"delay 15 seconds +838 "Can"m\\+zVt.& +665 ."PROCESSING"SPR.command "hold_off" +296 #Waiting up to 1:00:00 for SPR to enter state 7 +11014 "Can"k\\/+zQu1a & +11973 l\\+zSu.C& +11969 m] +zTu/(& +11971 k]1+zSu2>ƙ' +11973 k]<+zYu1T'5 +11972 k]+zbu1u'S +11978 k\\+zSu//N'q +11975 j\\+zTu.'' +11974 m\\+z[u$I' +11970 m\\[+zSu$3+' +11970 i[+zWu$-l' +11970 k[+zWu,b ( +11971 m[O+zTu\ $hH(% +11973 m[+z\\u'*(C +11972 k\\2+z[u0(a +4623 #"PROCESSING"SPR analysis completed +60 #"SPRlogger"Ended log spr26jul3003h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +110 "carousel0"F^% +3 "core" %FrbP \  +7 "sampler0"H=% +4 "core" Ƚ% +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"E & +3 "core" ʼn& N;eO* 9 +3 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" Ś( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 F% +3 "core" ƈ%FrSPK \  +4 ."detached from tina"Carousel.home.seek 17 +2 "carousel0"F9' +15 "core" ƹ' +2 "carousel0"F% +4 "core" Ɔ%6FvT 2 +3 "syringe1"C& +4 "core" Ð&ށ2 +3 ."detached from tina"Plunge.seek :locked +2 "valve0"DK'Z +5 "core" ' +2 "syringe0"BT& +5 "core" &)t & +4 ."Archive"Archive.in :unlocking +3 "syringe1"C>% +5 "syringe0"B? PZ-2 +4 "core" þ%6 b +9 ."Archive"Archive.seek :clear +6 ."Air"Air.in :unlocking +2 "syringe1"C[& + "syringe0"B<(j +3 "syringe1"C> P]z-2 +12 "core" &ށ2 +2 ."Air"Air.seek :clear +6 ."Lysis1"Lysis1.in :unlocking +5 "syringe1"C0' + C? P[(2 +3 "syringe0"Bu% +10 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C1( +5 "core" %ށ3h +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +3 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B6'j +60 "core" ð' +342 ¼( +23 ñ( +16 ¶' +4 "sampler0"H`% +3 "core" %. +59 ."detached from tina"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +2 .Gate.power :core,:OFF + .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785401289.09EDT @EDT1785401289.31 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +354 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +8 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 D +3 "core" ę +7 ."detached from tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +6 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +7 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +6 ."detached from tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +6 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default + "syringe1"C$d P[<2 +4 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +3 "valve0"D% +3 D& +6 "core" Ď%F6N  +2 "carousel0"F?% +5 "syringe0"BE& +9 "syringe1"CI% +6 C$& +4 ."Plunge_home"Plunge.home.jog 0 +5 "syringe0"Bo% +7 "valve1"Ep& +7 "valve0"D)) +26 "core" ą&<O) ` + ƿ%MFvQ ! + &׶n' ) + %݆0 ä&Ak! s + %ٕق  + &\\O! -+ + ĩ) +10 "valve0"D!% +9 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +2 ."Carousel_home"Carousel.home.jog 0 +3 ."Air_home"Air.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +3 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +2 "valve1"Eb* +2 "carousel0"F\\) +3 "syringe1"CN* +2 "syringe0"B* +3 "syringe1"C&) +2 "syringe0"Bv) +3 "valve0"D* +32 "core" ġ%GFO+  * + * + ) + ē* ‘* + æ) + ) +6 "syringe1"Ce& +8 CG% +5 "valve1"EB& +5 "valve0"D& +5 "syringe0"B % +3 Bk& +3 ."Plunge_home"Plunge.in :homing +8 "valve0"D> (2 +5 "core" &8r +2 %݃o  + &&\\O  + ă&BO Œ%qٔr  + &_ײ5  +8 ."Plunge_home"Plunge.jog 1493 +7 ."Lysis2_home"Lysis2.in :homing +8 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +2 ."PinPort_home"PinPort.in :homing +2 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 "syringe0"B> (2 +4 "valve0"Dm) +3 "syringe1"C> -2 +4 "valve0"D?2 +4 "valve1"E?2 +3 "syringe1"C? (2 +10 "syringe0"B? -2 +3 ."Lysis2_home"Lysis2.jog 1621 +6 ."Air_home"Air.jog 1432 +3 ."PinPort_home"PinPort.jog 1247 +3 ."Toroid_home"Toroid.jog 1352 +3 ."Lysis1_home"Lysis1.jog 275 +6 ."Archive_home"Archive.jog 312 +4 "syringe0"Bn)U +3 "syringe1"C3) +3 "valve1"E* +4 "syringe1"C* +2 "valve0"DP*H +8 "syringe0"BY*8 +44 "core" Ï* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"CJ( +3 "core" ) +4 ."Plunge_home"Plunge.setRawPosition! 50 +3 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"Dh' +5 "core" * +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +5 ."Archive_home"Archive.seek :home +3 "syringe0"B-( +5 "core" ( +3 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +7 "core" ' +2 ó) +2 ) +5 ."Air_home"Air.setRawPosition! 0 +3 ."Plunge_home"Plunge.in :default +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe1"C7 + "valve0"D> P ޤ-2 +2 "syringe0"B72 +11 ."Lysis2_home"Lysis2.seek :home +2 ."Air_home"Air.seek :home +56 "syringe0"BA' +8 "syringe1"CL' +8 "core" ­( + Ŗ* + ' +6 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Air_home"Air.in :default +2 ."Archive_home"Archive.in :default +3 "valve1"E8 N + "syringe1"C>d P]z<2 +3 "syringe0"B?d PZ<2 +10 ."PinPort_home"PinPort.seek :home +3 "valve1"El( +7 "core" ' +2 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +195 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"Dr( +5 "core" ( +2 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +209 "core" ( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +37 ."clearToroid"Toroid.seek :clear +2 "valve1"E/& +3 "valve0"D( +4 "core" ů&O . +8 ."detached from tina"PinPort.in :unlocking + "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E}( +41 "core" ( +299 Ă( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C~' +3 "core" ' +4 ."detached from tina"Lysis1.seek :clear +2 "syringe1"C( +67 "core" ( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"BZ'j +74 "core" ' +3 ."detached from tina"Archive.seek :clear +2 "syringe0"B(j +73 "core" ( +3 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 FH% +4 "core" %QFxQ  +4 ."detached from tina"Carousel.home.seek 17 +2 "carousel0"FX' +14 "core" ' +3 "valve0"Dg% +5 "core" %^oO. ! +3 #"detached from tina"Selecting Cartridge 12 +2 "valve0"D5% +4 "core" ĵ%^eO2 ! +3 "syringe1"C;& +4 "core" û&?^ +2 "syringe1"C % +5 "core" à%ݍ +3 "valve1"Ed& +3 "core" &O. t +4 ."detached from tina"Carousel.seek 12 +2 "carousel0"F\ '\ +125 "core" Ɗ' +4 "carousel0"Fq% +3 "core" %\ )1S 2 +3 ."detached from tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"C"& +3 "core" â&;]  +4 ."detached from tina"Lysis1.in :fast + "syringe1"C?d P[<2 +4 ."detached from tina"Lysis1.seek :locked +3 "syringe1"Cx( +247 "core" ( +3 "valve0"D,% +7 "core" Ĭ%^O ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"D2'Z +5 "syringe1"Cw% +7 "core" %ݍV +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +5 ."Air"Air.seek :locked +2 "syringe1"CW'6 +83 "core" IJ' +19 ' +3 ."detached from tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +4 F#% +3 "core" ƣ%\ X1 eQ  +7 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default + "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" 4y +4 "cartridge" yՎՎ +3 "Cartridge" (y  +5 "cartridge" y /J59971 Z/MC% +3 #"detached from tina"Mounted lysis Cartridge board#J59971 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D_% +3 "core" %ZFO- y +3 "syringe1"C& +4 "core" Ô&W +3 "syringe1"C % +4 "core" Í%6ɺZ +3 "carousel0"F7% +3 "core" Ʒ%\ 1 PX \ +4 "carousel0"FM% +6 "core" %\ 1 P^ \ +120 ."PROCESSING"SPR.command "read_sequences" +22 .SPR.command "stats_on" +7 "carousel0"Fs% +3 "core" %\ 1 Pm \ +5 ."PROCESSING"SPR.command "start 0 1" +76 ."SPRlogger"SPR.start /var/log/esp/spr26jul3004h.spr.bz2 +195 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste + "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Ca% +3 "core" %6T +47 "syringe1"CQ% +3 "core" %6ɽR +47 "syringe1"C% +3 "core" Ý%6ɻK +47 "syringe1"C% +5 "core" Ü%6ɽO +45 "syringe1"C% +4 "core" Û%6ɻN +46 "syringe1"C%% +4 "core" å%6ɹO +46 "syringe1"CR% +4 "core" %6ɼO +46 "syringe1"C% +4 "core" Þ%6ɹO +47 "syringe1"CF% +3 "core" %6P +47 "syringe1"C% +3 "core" ×%6ɻɂ" +47 "syringe1"Cf% +4 "core" %6ɼQ" +46 "syringe1"C@% +3 "core" %6ɿQ\ +47 "syringe1"C|% +3 "core" %6ɼO +47 "syringe1"CO% +4 "core" %6ɼQ +5 "Can"k\\2+z[u&2({ +3 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +3 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"Ez( N +78 "core" ( +4 ."posArchive"Archive.in :fast +9 ."posLyS2"Lysis2.in :fast +6 "syringe0"B?d PZ<2 + B>d P\\k<2 +2 ."PROCESSING"Lysis1.seek 800uL +6 "syringe1"Cy(% +7 ."posArchive"Archive.seek :empty +6 ."posLyS2"Lysis2.seek 800uL +6 "syringe0"B+( +4 B.'% +398 "core" ( +154 ®' +277 «( +3 ."PROCESSING"delayUntil "04:58:46.96" +817 "Can"l\\Q+z^u7N( +11970 n\\?+zTu<c( +11970 l\\$+zUu<9( +11969 l\\3+z_u@5( +11970 l\\+z`u?-( +9574 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 B % +3 "core" ‹%%n3 [ +4 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +6 Ci& +5 "core" &%n 2 h +54 ."Lysis2mixer"Lysis2.seek 550uL +6 ."PROCESSING"Lysis1.seek 550uL +4 "syringe0"BC' + "syringe1"C( +2308 "Can"k\\\ +zasa) +1179 "core" Ø( +4 ' +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +130 #SPR stabilized + "syringe1"Cc& +3 "core" &XX +3 "syringe0"B8% +6 "core" ¸%X +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +264 #Waiting up to 11:00 for SPR to enter state 1 +7821 "Can"k[+zfu1 )3 +3948 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"BV% +4 "core" %X +3 "syringe1"C'& +6 "core" ç&X +50 ."Lysis2mixer"Lysis2.seek 250uL +4 ."PROCESSING"Lysis1.seek 250uL +3 "syringe0"BD' +3 "syringe1"C( +4180 "core" ' +4 Ä( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +342 #Waiting up to 1:00:00 for SPR to enter state 7 +1726 "Can"l[c+zWt.)Q +11971 m[+zRu+,)o +11970 jZ+zRu*'~) +11970 m[>+zSu0) +11971 j[+zYu0*!) +11966 m\\+zmu0%) +11972 k\\+zau2(* +11971 j\\+zXu2,**# +11946 #"SPRlogger"SPRsummary:5.74RIU,13.5RIU,3.4RIU,19.2RIU after 1924s +23 "Can"k[+z^u$/*A +11969 n[+zVu $#3*_ +11964 k[+zTu$'8(*} +11969 k[+z_u0SY+m +11968 l\\+zqu,'\\+ +11964 l]8+zfu0\\` + +11969 k\\+zSu18c+ +11970 l\\{+zWu1Pg9+ +11966 k\\7+z_u2j, +11967 k[+zmu0 n6,! +11969 j[t+zeu $q,? +11969 l[$+z\\u$Av,] +11968 k[O+zbu $uzU,{ +11969 k[+zeu/~\\, +11968 k\\+zfu$-, +11968 k\\>+ziu$mm, +11967 l\\E+z]u.q\ , +11966 #"PROCESSING"SPR analysis completed +6 "Can"j\\-+z^u4- +47 #"SPRlogger"Ended log spr26jul3004h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +123 "carousel0"F % +5 "core" Ɖ%\ 1 Pz \ +7 "sampler0"H% +4 "core" Ȉ%7 +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +48 ."detached from tina"Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +2 "valve1"E& +3 "core" ņ& N;O'  +4 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +2 "valve1"E( +78 "core" Ő( +2 ."detached from tina"Carousel.in :default +2 "carousel0"F><` F 22 +4 FT% +3 "core" %\ 1 OP< \ +4 ."detached from tina"Carousel.home.seek 12 +3 "carousel0"F>'\ +14 "core" ƾ' +2 "carousel0"F[% +4 "core" %\ 11 T 2 +3 "syringe1"Cu& +4 "core" &ށ2 +3 ."detached from tina"Plunge.seek :locked +2 "valve0"Dj'Z +5 "core" ' +2 "syringe0"BU& +5 "core" &)m* - +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +3 "syringe1"CS% +6 ."Archive"Archive.seek :clear +2 "syringe0"B(j +4 "core" %6g  +6 ."Air"Air.in :unlocking +3 "syringe1"C]& +4 C> P]z-2 +4 "core" &ށ2 +2 ."Air"Air.seek :clear +7 ."Lysis1"Lysis1.in :unlocking +2 "syringe1"C' +3 "syringe0"B:% +3 "syringe1"C? P[(2 +10 ."Lysis1"Lysis1.seek :clear +2 "syringe1"C`( +2 "core" º%ށ3j؁ +7 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"B'j +60 "core" Õ' +334 ’( +31 ( +17 ™' +4 "sampler0"H% +4 "core" Ȏ%_ +11 ."detached from tina"Gate.power :motor,:OFF +49 .Gate.power :analytic1,:OFF +5 .Gate.power :core,:OFF + .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785406030.33EDT @EDT1785406030.54 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +355 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +8 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 Dp +3 "core"  +8 ."detached from tina"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from tina"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from tina"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from tina"Carousel.configure :default + "carousel0"F#<` F 22 +7 ."detached from tina"Abs.configure :default +2 "ambient0"J#  +6 ."detached from tina"PhaseSeparator.configure :default +2 "carousel1"G$ +6 ."detached from tina"Archive.configure :default +3 "syringe0"B$d PZ<2 +6 ."detached from tina"Air.configure :default +2 "syringe1"C#d P]z<2 +4 ."detached from tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +3 ."detached from tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from tina"PinPort.configure :default +2 "valve1"E$ B22 +3 #"detached from tina"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"Do% +3 D$& +3 "core" %FO"  +4 "carousel0"FI% +8 ."Plunge_home"Plunge.home.jog 0 +9 "syringe0"B?& +4 "syringe1"C% +3 CE& +4 "valve0"D!) +9 "syringe0"BB% +3 "valve1"E & +22 "core" Ĥ&PO& L + %L1\ Q Ѝ + ¿&קl: 8 + Å%ݏ0( + &0kD  + %{_  + Ō&n*qN - ġ) +9 "valve0"De% +5 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +2 ."Lysis1_home"Lysis1.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +4 "syringe0"BG) +2 "syringe1"Ck) +4 "carousel0"F) +7 "syringe0"BK* +2 "valve0"D* +5 "syringe1"C1* +2 "valve1"E9* +29 "core" %EFO# + ) + ) + * + ñ* Ě* + Ź* + ƃ) +2 "valve1"E0& +5 "syringe1"C<& +7 "syringe0"B6& +3 "valve0"D{& +4 "syringe0"B)% +4 "syringe1"Cv% +6 ."Plunge_home"Plunge.in :homing +4 "valve0"D> (2 +22 ."Plunge_home"Plunge.jog 1495 +3 "valve0"D&) +3 "core" Ű&%kO  + ü&:k + ¶&^׫l3  &SO + ©%nَi + %݊( +12 ."PinPort_home"PinPort.in :homing +3 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +3 ."Lysis1_home"Lysis1.in :homing +2 ."Archive_home"Archive.in :homing +2 "syringe0"B> (2 +2 "valve1"E?2 +6 "syringe1"C> -2 +2 "valve0"D?2 +5 "syringe1"C? (2 +4 "syringe0"B? -2 +8 ."PinPort_home"PinPort.jog 1245 +5 ."Lysis2_home"Lysis2.jog 1622 +5 ."Air_home"Air.jog 1431 +3 ."Lysis1_home"Lysis1.jog 275 +2 ."Toroid_home"Toroid.jog 1355 +3 "valve1"Eb* +3 ."Archive_home"Archive.jog 314 +9 "syringe0"B)V +4 "syringe1"CN) +2 C* +2 "valve0"D*K +3 "syringe0"Bm*: +23 "core" Ħ) +4 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +3 "valve0"DY' +16 "core" Ñ* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +4 "core" * + ' +3 ."Archive_home"Archive.setRawPosition! 50 +4 ."Plunge_home"Plunge.in :default +2 ."Lysis1_home"Lysis1.seek :home +2 "syringe0"B82 +2 "valve0"D> P ޤ-2 +4 "syringe1"Cn( +9 ."Archive_home"Archive.seek :home +5 "syringe0"B3( +17 "core" ( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +6 "core" ) + ³( +4 ."Archive_home"Archive.in :default +3 ."Air_home"Air.setRawPosition! 0 +2 "syringe0"B?d PZ<2 +2 "syringe1"C7 +5 ."Air_home"Air.seek :home +55 "syringe1"C' +3 "core" “) + * +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +12 ."PinPort_home"PinPort.setRawPosition! 3150 + "syringe0"B72 +3 "valve1"E8 N +5 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"B/' +4 ."PinPort_home"PinPort.seek :home +6 "valve1"EP( +9 "core" Ö' +3 ."Air_home"Air.in :default +2 "syringe1"C>d P]z<2 +5 "core" ¯' +3 ."Lysis2_home"Lysis2.in :default + "syringe0"B>d P\\k<2 +190 "core" ď* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"Dr( +3 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +204 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +3 "valve1"El& +2 "valve0"DH( +6 "core" &O  +7 ."detached from tina"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from tina"PinPort.seek :clear +3 "valve1"Eg( +40 "core" ( +293 ( +3 ."detached from tina"Air.seek :clear +2 "syringe1"C5' +5 "core" õ' +2 ."detached from tina"Lysis1.seek :clear +3 "syringe1"C;( +67 "core" û( +3 ."detached from tina"Lysis2.seek :clear +2 "syringe0"B 'j +74 "core"  ' +3 ."detached from tina"Archive.seek :clear +3 "syringe0"Bd(j +73 "core" ( +3 ."detached from tina"Carousel.in :default + "carousel0"F><` F 22 +4 FL% +3 "core" %\ Z1 Q  +4 ."detached from tina"Carousel.home.seek 12 +2 "carousel0"FJ'\ +15 "core" ' +2 "valve0"DA% +4 "core" %^pO* ! +4 #"detached from tina"Selecting Cartridge 6 +2 "valve0"Dq% +4 "core" %^bO, ! +3 "syringe1"C"& +4 "core" â&<_&  +3 "syringe1"C-% +4 "core" í%݋Z& +3 "valve1"E\\& +4 "core" &O# I +4 ."detached from tina"Carousel.seek 6 +3 "carousel0"FX' +147 "core" ' +4 "carousel0"F,% +4 "core" Ƭ%*5S 2 +3 ."detached from tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +4 "syringe1"Cw& +4 "core" &JX# +3 ."detached from tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +3 ."detached from tina"Lysis1.seek :locked +3 "syringe1"C( +247 "core" ( +3 "valve0"D#% +8 "core" ģ%^O !l +2 ."Plunge"Plunge.seek :locked +2 "valve0"Dh'Z +4 "syringe1"C~% +9 "core" %ݍ[ +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C4'6 +83 "core" ' +20 ô' +2 ."detached from tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F(% +3 "core" ƨ%V2]P  +8 #"detached from tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +6 ."detached from tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" _y +3 "cartridge" yִjִj +4 "Cartridge" y  +4 "cartridge" y r/J59943 r/6Cr +4 #"detached from tina"Mounted lysis Cartridge board#J59943 +3 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 "valve0"D % +3 "core" č%ZGO# U +3 "syringe1"C7& +5 "core" ÷&\\  +3 "syringe1"CM% +3 "core" %6Y +3 "carousel0"Fs% +6 "core" %2PJ \ +3 "carousel0"Fa% +6 "core" %2rPI \ +116 ."PROCESSING"SPR.command "read_sequences" +8 .SPR.command "stats_on" +7 "carousel0"FQ% +3 "core" %2mPI \ +56 ."PROCESSING"SPR.command "start 0 1" +20 ."SPRlogger"SPR.start /var/log/esp/spr26jul3006h.spr.bz2 +199 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +3 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C% +4 "core" Ý%6R +47 "syringe1"C% +3 "core" Ü%6ɽO +47 "syringe1"C% +3 "core" Û%6N +47 "syringe1"C%% +3 "core" å%6M% +47 "syringe1"CR% +3 "core" %6N' +47 "syringe1"C% +4 "core" Þ%6M' +46 "syringe1"CF% +3 "core" %6M- +47 "syringe1"C% +4 "core" ×%6L+ +47 "syringe1"Cf% +3 "core" %6ɾL% +47 "syringe1"C@% +3 "core" %6ɼM +47 "syringe1"C|% +3 "core" %6Q +47 "syringe1"CO% +4 "core" %6J +46 "syringe1"C}% +3 "core" %6I +48 "syringe1"Cx% +5 "core" %6H +44 "syringe1"C% +3 "core" Á%6H +47 "syringe1"CZ% +4 "core" %6ɿK +46 "syringe1"C % +3 "core" Ë%6H +47 "syringe1"Ci% +3 "core" %6H +47 "syringe1"C.% +3 "core" î%6m1 +47 "syringe1"Cy% +3 "core" %6I +47 "syringe1"Cc% +3 "core" %6ɿK$ +5 "Can"j\\-+z^u(- +2 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted 0%) +2 .PinPort.in :locking +2 "valve1"E? B22 +3 ."PROCESSING"PinPort.seek :locked +2 "valve1"E8( N +78 "core" Ÿ( +4 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 800uL +3 ."posArchive"Archive.seek :empty +5 "syringe0"B>d P\\k<2 +4 "syringe1"CV(% +2 "syringe0"B'( +10 ."posLyS2"Lysis2.seek 800uL +7 "syringe0"BC'% +397 "core" ( +153 ' +266 §( +3 ."PROCESSING"delayUntil "06:17:48.20" +5107 "Can"m[+zSu:u -/ +11971 k[+z^u@j>-M +11970 i[+z^u9s-k +11966 l[a+zVu9- +11973 k[)+zru=X- +4945 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +3 B% +3 "core" ˜%%n*# X +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 C& +3 "core" Ç&%n1\ n +56 ."Lysis2mixer"Lysis2.seek 550uL +7 ."PROCESSING"Lysis1.seek 550uL +2 "syringe0"B' +3 "syringe1"C*( +3484 "core" Ÿ' +5 ê( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +133 #SPR stabilized + "syringe1"Ct& +3 "core" &X +4 "syringe0"BD% +4 "core" %Y) +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +264 #Waiting up to 11:00 for SPR to enter state 1 +482 "Can"m[0+zYu0\ w- +11287 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B% +4 "core" „%Yv +4 "syringe1"C & +5 "core" É&Xr +56 ."Lysis2mixer"Lysis2.seek 250uL +5 "syringe0"B' +4 ."PROCESSING"Lysis1.seek 250uL +5 "syringe1"C( +463 "Can"h[i+zks/scj- +3709 "core" ˆ' +7 Æ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +349 #Waiting up to 1:00:00 for SPR to enter state 7 +6402 "Can"j\\+zmu1P. +11970 m]+znu0. +11969 l]z+zlu1Z.= +11971 k\\e+zju().[ +11969 k[+z`u1].y +11967 kZ^+zru/. +11967 mYo+z{u.Ņ. +11964 kY+zsu0. +7422 #"SPRlogger"SPRsummary:6.69RIU,12.1RIU,-0.432RIU,16.6RIU after 1925s +4544 "Can"mZ+zou $c. +11968 i\\0+zpu$j\ / +11968 k]@+zju$NG/- +11973 l]+zou/(/K +11969 l]O+zru$2!/i +11971 k\\+zru2gG/ +11969 l\\+zlu1/ +9326 "syringe1"CT& +5 "core" &?S +3 "syringe0"B[% +6 "core" %? +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +317 "Can"j[+zru13V/ +11449 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 + "syringe0"Bu% +6 "core" %?T +5 "syringe1"CU& +6 "core" &?LQ#  +60 ."Lysis2mixer"Lysis2.seek 100uL +4 "syringe0"BS'ށ +2 ."PROCESSING"Lysis1.seek 100uL +6 "syringe1"C](ށ +290 "Can"k[k+zgs>q;&/ +1799 "core" ' +5 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +294 #Waiting up to 1:00:00 for SPR to enter state 7 +8363 "Can"m[+ziu-[/ +11968 iZ+zsu/0 +11967 kZ+zu09a0; +11967 mZO+zru00Y +11968 jZ+zqu1g0w +11970 mZa+ztu10 +11970 k[+zru10 +11971 k[ +zqu0 0 +11970 l[D+zpu$|0 +11968 l[O+zqu $OA1 +11968 m[`+zqu $1+ +11971 i[Q+zpu.~E1I +11970 i[^+zru$Z1g +11971 k[+zpu-\\#c1 +11973 mZ+zqu1&1 +7315 #"PROCESSING"SPR analysis completed +53 #"SPRlogger"Ended log spr26jul3006h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +123 "carousel0"F:% +3 "core" ƺ%0PU \  +7 "sampler0"Hz% +4 "core" %' +4 ."closeHandoff"Handoff.select :close +3 "valve0"D +49 ."detached from tina"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +2 "valve1"E\ & +4 "core" Ŋ& N<` F 22 +4 F+% +3 "core" ƫ%0Pb \  +4 ."detached from tina"Carousel.home.seek 6 +3 "carousel0"FW' +14 "core" ' +2 "carousel0"F=% +4 "core" ƽ%10T 2 +3 "syringe1"C^& +4 "core" &ށ2 +3 ."detached from tina"Plunge.seek :locked +2 "valve0"D'Z +5 "core" Ď' +2 "syringe0"Bp& +5 "core" &)y  +4 ."Archive"Archive.in :unlocking +3 "syringe1"Co% +5 "syringe0"B? PZ-2 +3 "core" %6u% +4 ."Archive"Archive.seek :clear +6 ."Air"Air.in :unlocking +3 "syringe0"B (j +2 "syringe1"CB& +5 C> P]z-2 +6 "core" &ށ2n +2 ."Air"Air.seek :clear +5 "syringe1"CE' +6 ."Lysis1"Lysis1.in :unlocking +4 "syringe0"B% +2 "syringe1"C? P[(2 +6 "core" …%ށ|3p +4 ."Lysis1"Lysis1.seek :clear +5 "syringe1"C?( +2 ."Lysis2"Lysis2.in :unlocking +6 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"BI'j +56 "core" ' +345 Œ( +30 ÿ( +10 ' +4 "sampler0"H$% +3 "core" Ȥ%U +11 ."detached from tina"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF + #Safely stopped and ready to power off @1785410771.07EDT @EDT1785410771.66 !o:Cartridge::None: mesg"*Not enough standard cartridges remain:bt["7/home/esp/esp2/bin/fireAll:291:in `checkInventory'"-/home/esp/esp2/bin/fireAll:289:in `each'"7/home/esp/esp2/bin/fireAll:289:in `checkInventory'"#/home/esp/esp2/bin/fireAll:318"-/home/esp/esp2/bin/fireAll:317:in `loop'"#/home/esp/esp2/bin/fireAll:317";/home/esp/esp2/lib/framework.rb:199:in `instance_exec'"7/home/esp/esp2/lib/framework.rb:199:in `runScript'"4/home/esp/esp2/utils/init3g.rb:277:in `fireAll'"!tina:12:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3638 "Can"lZ+zbu)1 +11973 mZ+ztu*U1 +11970 jZ+ztu*1 +11972 kZ+zru+j2 +11971 jZ+zwu+29 +11970 m[n+zu,2W +11971 m\\>+zsu- 2u +11975 k\\+zuu-2 +11971 k]l+zvu.#2 +11973 k]+z~u.2 +11973 j]m+ztu/82 +11972 m]+zsu/3 +11971 l\\+zsu0O3) +11972 k\\+zuu03G +11974 l\\+zqu1f3e +11974 j\\+zvu13 +11975 l\\+zsu2}3 +11975 j\\{+zvu33 +11975 l\\+zu33 +11975 m\\+zu43 +11973 k\\+zru44 +11974 l\\+zu5347 +11974 k\\+zru54U +11975 m\\+zu6J4s +11974 m\\+zwu64 +11976 k\\+zu7_4 +11977 k\\+zu74 +11972 l\\+zu8t4 +11970 k]0+zu85 +11972 l]F+zzu95' +11971 l]I+zu:5E +11968 l]$+z~u:5c +11968 m\\/+zuu;(5 +11968 m[+zu;5 +11970 l[+zu<=5 +11972 m\\+zu<5 +11973 j]+zu=S5 +11973 m]+zu=6 +11970 k]'+zu>i65 +11970 l\\+zu>6S +11970 k\\+zsu?6q +11969 m\\+zxu@ 6 +11969 j[+zyu@6 +11967 l\\+zuA!6 +11971 o]4+zuA6 +11966 m]+zuB67 +11969 l^ +zuB7% +11965 k]+zuCK7C +11969 m]A+zuC7a +11971 l\\+zuD`7 +11970 k\\+zuD7 +11968 l\\R+zuEv7 +11967 l[+zuF7 +11965 l\\+zuF7 +11967 k]d+z~uG8 +11968 l^+zuG83 +11967 k^d+zuH.8Q +11968 l^+zuH8o +10393 #"preston"Session Begun +499 #-> ESP.ready1 +5 !o:NoMethodError\ : mesgu:NameError::message-undefined method `ready1' for ESP:Module:@timestampu: Time ͛h|X: name: ready1: args[:bt["#preston:1:in `topLevelBinding'" ESP.ready! +10 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +12 #Powering core bus +238 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +26 "valve0"DP +3 De +3 "core"  +7 ."preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."preston"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."preston"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."preston"Abs.configure :default +2 "ambient0"J#  +9 ."preston"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."preston"Air.configure :default +3 "syringe1"C#d P]z<2 +6 ."preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +9 ."preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 ."preston"PinPort.configure :default +2 "valve1"E$ B22 +6 #"preston"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"D0% +3 D)& +4 "core" İ%FO  +3 "carousel0"F<% +7 "syringe0"Bv& +4 ."Plunge_home"Plunge.home.jog 0 +16 "syringe1"C6% +7 C{& +4 "syringe0"Bj% +3 "valve1"E& +4 "valve0"D) +20 "core" ĩ&O + Ƽ%L3Q d + &k0  ö%݌i9 + &<*+ x + %ِ)7  + ř&O , ĕ) +11 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +6 ."Toroid_home"Toroid.home.jog 0 +7 "valve0"D>% +4 ."PinPort_home"PinPort.home.jog 0 +5 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +5 "syringe0"B) +5 "syringe1"C* +4 "syringe0"B`* +9 "valve0"D* +3 "valve1"E!* +4 "syringe1"C9) +18 "carousel0"FG) +10 "core" ľ%DFO + ’) + Â* + * + Đ* + š* + ù) +6 "syringe0"B1% +5 "syringe1"C& +4 "valve1"Ek& +3 "syringe1"CK% +2 "valve0"D&& +8 "syringe0"B& +2 ."Plunge_home"Plunge.in :homing +4 "valve0"D> (2 +22 ."Plunge_home"Plunge.jog 1501 +9 "valve0"Dm) +3 "core" ) +2 ±%oْj + Ú&4/ &O + %|)  + Ħ&?O  + ‘&bj +6 ."Archive_home"Archive.in :homing +11 ."Lysis1_home"Lysis1.in :homing +5 ."Lysis2_home"Lysis2.in :homing +6 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +6 ."PinPort_home"PinPort.in :homing +4 "syringe0"B? -2 +2 "syringe1"C? (2 +5 "syringe0"B> (2 +6 "syringe1"C> -2 +5 "valve0"D?2 + "valve1"E?2 +7 ."Lysis1_home"Lysis1.jog 276 +3 ."Archive_home"Archive.jog 307 +6 ."Lysis2_home"Lysis2.jog 1621 +8 ."Air_home"Air.jog 1434 +4 ."Toroid_home"Toroid.jog 1363 +4 ."PinPort_home"PinPort.jog 1236 +6 "syringe1"CN* +11 "syringe0"B*3 +4 Bb)U "valve0"D*S + "valve1"Er* +2 "Can"k^+zt0+Io8 + "syringe1"Cl) +29 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +5 ."Plunge_home"Plunge.seek :home +4 "valve0"DP' +8 "core" * +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +6 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"CL( +8 "core" “* + ' +3 ."Archive_home"Archive.setRawPosition! 50 +2 ."Plunge_home"Plunge.in :default +3 "syringe0"B82 +2 "valve0"D> P ޤ-2 +10 ."Archive_home"Archive.seek :home +3 "syringe0"BA( +6 "core" ( +3 ."Lysis1_home"Lysis1.in :default + "syringe1"C?d P[<2 +15 "core" ) +2 ( +3 ."Archive_home"Archive.in :default +2 ."Air_home"Air.setRawPosition! 0 +4 "syringe0"B?d PZ<2 +4 "syringe1"C7 +4 "core" ) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +5 ."Air_home"Air.seek :home +4 "syringe0"B72 +7 "syringe1"Cq' +5 ."Lysis2_home"Lysis2.seek :home +6 "syringe0"B"' +7 "core" ' + * +3 ."Air_home"Air.in :default +3 ."PinPort_home"PinPort.setRawPosition! 3150 +7 "syringe1"C>d P]z<2 +2 "valve1"E8 N +10 ."PinPort_home"PinPort.seek :home +4 "valve1"E-( +8 "core" ¢' +42 ."Lysis2_home"Lysis2.in :default +12 "syringe0"B>d P\\k<2 +182 "core" ď* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D\\( +7 "core" ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +166 "core" ŭ( +2 ."PinPort_home"PinPort.in :default +3 "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +4 "valve0"Dn( +6 "core" Ŗ&AO +5 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +8 ."preston"PinPort.seek :clear +2 "valve1"E/( +41 "core" ů( +291 ( +2 ."preston"Air.seek :clear +5 "syringe1"C3' +5 "core" ó' +2 ."preston"Lysis1.seek :clear +3 "syringe1"C,( +69 "core" ì( +3 ."preston"Lysis2.seek :clear +5 "syringe0"Bw'j +74 "core" ' +3 ."preston"Archive.seek :clear +3 "syringe0"B(j +75 "core" ƒ( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +6 FJ% +3 "core" %T4P  +5 ."preston"Carousel.home.seek 6 +5 "carousel0"F#' +15 "core" ƣ' +2 "valve0"D~% +5 "core" %^ O1 !y +3 "valve0"D;% +3 "core" Ļ%^ O0 !t +4 "sampler0"H(% +3 "core" Ȩ%F" +4 "valve0"DY& +5 "core" &3 O/  +3 "carousel0"F5% +5 "core" Ƶ%M5P  +2 "ambient0"J_% +5 "core" %| +2 "carousel1"G& +5 "core" ǔ&LP +2 "syringe0"B & +8 "core" &j׿W + +3 "syringe1"C7% +5 "core" ÷%݊\\ +2 "syringe1"CM& +5 "core" &GL +2 "syringe0"Bs% +5 "core" %jٓU +3 "valve1"Ea& +3 "core" &  O0  +4217 #"preston"-> SC.to 25 +5 .Carousel.seek 25 +3 "carousel0"FQ' +413 "core" ' +2 "carousel0"F% +4 "core" Ɲ%4hS 2 +5893 "Can"l^+zuBL8 +3140 #"preston"-> SC.to 40 +6 .Carousel.seek 40 +3 "carousel0"F'& +329 "core" Ɯ' +2 "carousel0"F% +4 "core" ƛ%&'lS 2\ +3384 #"preston"-> SC.to 50 +5 .Carousel.seek 50 +3 "carousel0"F%'/ +228 "core" ƥ' +2 "carousel0"FR% +5 "core" %/(@|S 2 +4857 "Can"k^+zuPO8 +6342 #"preston"-> exit +3 #Session Ended +5620 "Can"k^2+zuNKR8 +11964 l]+zuQ?U9 +11966 k]m+zuPDX9# +11965 k]+zuO1[9A +11967 m^+zuNF^9_ +11966 j^+zuOEa9} +11967 l_o+zuN@d9 +11967 k_I+zuOFg9 +11965 k^+zuM6j9 +11962 l^e+zuODm9 +11965 m^+zuM.pv: +11964 l^+zu\ +r:1 +11964 k_+zu\ 0s1:O +11967 ka$+zu\ 2ta:m +11964 l`+zu\ *u: +11963 l`i+zu\ .v: +11965 j_+zu\ #w: +11965 n_+zu\ 2y$: +11964 k_&+zu\ zT; +11965 m`+zu\ {;! +11966 ka+zu\ |;? +11966 ja+zu\ .};] +11966 o`+zu\ ;{ +11969 n_+zu\ +H; +11966 k_?+zu\ "x; +11968 l_+zu\ :; +11967 k_y+zu\ ); +11964 j`+zu\ $\ < +11966 la+zu\ ; +11969 o^+zu\ 0w>- +11970 l^+zu\ ,>K +11970 j^+zu\ ">i +11970 l^+zu\ - > +11969 n_V+zuN,\\> +11973 j_+zuO1P> +11978 l_c+zuKED> +11974 m_,zuJt9> +11969 n_+zuN4.? +3741 #"Tina"Session Begun +8231 "Can"l_H+zuMF$?; +5648 #"Tina"-> slot +2482 #-> slot1..60=>nil +3 !o:SyntaxError: mesg"fcompile error\ Tina:2: syntax error, unexpected tASSOC, expecting $end\ slot1..60=>nil\ ^:@timestampu: Time ϛE:bt[" Tina:2:in `topLevelBinding'" slot 1..60=>nil +496 #-> slot +415 "Can"k_+zuL2?Y +4795 #"Tina"-> slot 53=>[:microSPR_phyto, :standard, {lysed:1000, lysed2:1000}] +1390 #-> Cart.aspects +5785 "Can"l_+zuMB?w +9925 #"Tina"-> Cart.aspectsOf :standard, extraDilutions:[1,1], lysed:950, lysed2:950 +2049 "Can"m_y+zuMC? +1246 #"Tina"-> slot 53=>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +3743 #-> to 53 +4 "valve0"D% +3 "core" Ğ%^O5 !m +3 "syringe1"CF& +4 "core" &\\p +3 "syringe1"C% +4 "core" ×%ݔv +3 "valve1"Ef& +3 "core" &O1  +4 ."Tina"Carousel.seek 53 +2 "carousel0"F@'2 +86 "core" ' +5 "carousel0"F|% +3 "core" %25T* 2 +3 ."Tina"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"CO& +3 "core" &_p +4 ."Tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +8 ."Tina"Lysis1.seek :locked +7 "syringe1"C}( +248 "core" ( +3 "valve0"Dx% +8 "core" %^OP !k +2 ."Plunge"Plunge.seek :locked +3 "valve0"D'Z +58 "syringe1"CZ% +7 "core" %ݗu +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +5 "syringe1"C '6 +27 "core" ā' +76 Ë' +3 ."Tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 Fi% +3 "core" %2ZQF  +8 #"Tina"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."Tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."Tina"PuckHeater.configure :default +4 "Cartridge" \ :D H  +6 ."Tina"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" .y +3 "cartridge" yAA +5 "Cartridge" yy  +4 "cartridge" y a"&0J59949 Y%&0lC/q +6 #"Tina"Mounted lysis Cartridge board#J59949 +9 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +2201 #-> PhytoSampler.prepareLGcart :full, :full, :full +2 .Handoff.select :waste +2 "valve0"D +50 #"Tina"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cc% +6 "core" %6_ +45 "syringe1"Cg% +3 "core" %6\\ +47 "syringe1"C% +3 "core" %6] | +46 "syringe1"Cd% +3 "core" %6[ +47 "syringe1"C % +3 "core" à%6W +47 "syringe1"C% +4 "core" Ø%6T +46 "syringe1"C% +3 "core" Ç%6V } +48 "syringe1"CC% +4 "core" %6U +46 "syringe1"CV% +3 "core" %6U +47 "syringe1"Ct% +4 "core" %6S +46 "syringe1"CD% +3 "core" %6W +47 "syringe1"C% +3 "core" Ä%6W +47 "syringe1"C % +3 "core" É%6P  +47 "syringe1"C% +3 "core" ß%6T +5 "Can"m_y+zu2R?7 +2 #"Tina"Syringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking +3 "valve1"E? B22 +7 ."Tina"PinPort.seek :locked +3 "valve1"E*( N +80 "core" Ū( +4 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +6 ."Tina"Lysis1.seek :full +7 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :full +5 "syringe1"CT( +5 "syringe0"B[( +3 ."posLyS2"Lysis2.seek :full +14 "syringe0"Bu' +150 "core" ( +19 ( +17 ' +3 "syringe0"BU& +4 "core" &~ʁ 8 +2755 "Can"l_^+zuHK? +1116 #"Tina"-> Archive.empty +4 .Archive.in :default +3 "syringe0"B?d PZ<2 +5 ."Tina"Archive.seek :empty +4 "syringe0"B( +1653 "core" ˆ( +2 "syringe0"B& +4 "core" †&A) < +1625 #"Tina"-> Lysis2.to 1000 +3 .Lysis2.in :default +4 "syringe0"B>d P\\k<2 +6 ."Tina"Lysis2.seek 1000uL +3 "syringe0"B:'y +785 "core" º' +2 "syringe0"Bz% +3 "core" %yB~ρ < +1335 #"Tina"-> Lysis1.to 1000 +5 .Lysis1.in :default + "syringe1"C?d P[<2 +7 ."Tina"Lysis1.seek 1000uL +8 "syringe1"C\ (y +783 "core" Ê( +2 "syringe1"C+& +4 "core" ë&y~ < +505 #"Tina"-> Cart.unlock +6 .Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +3 "valve1"E=& +7 "core" Ž& N;b O3 < +3 ."Tina"PinPort.in :unlocking +2 "valve1"E? B2 +5 ."Tina"PinPort.seek :clear +3 "valve1"E^( +80 "core" ( +2 ."Tina"Carousel.in :default +4 "carousel0"F><` F 22 +6 Fp% +2 "core" %2MPV \  +4 ."Tina"Carousel.home.seek 53 +2 "carousel0"Fo'2 +15 "core" ' +3 "carousel0"FB% +3 "core" %2(S 2 +3 "syringe0"B& +4 "core" …&) +5 ."Archive"Archive.in :unlocking +3 "syringe1"C8% +4 "syringe0"B? PZ-2 +7 "core" ø%6 +5 ."Archive"Archive.seek :clear +6 ."Air"Air.in :unlocking +5 "syringe0"BX% +2 Bh(j +2 "syringe1"C> P]z-2 +10 ."Air"Air.seek :clear +49 "syringe1"C'' +3 "core" %y~ +15 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +4 "syringe0"B4'j +63 "core" ç' +200 ´' +112 ( +2 "syringe1"C]& +3 "core" &y~} +6 ."Lysis1"Lysis1.in :unlocking +3 "valve0"DS% +5 "syringe1"C? P[(2 +5 "core" %ZGJO!  +4 ."Lysis1"Lysis1.seek :clear +5 "syringe1"C$( +8 ."Plunge"Plunge.in :unlocking +6 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"De'8 +97 "core" ' +150 ä( +3136 "Can"m_4+zzu64? +1108 #"Tina"-> cart.aspects +5 !o:NoMethodError\ : mesgu:NameError::message0undefined method `aspects' for :cart:Symbol:@timestampu: Time ϛNEi: name: aspects: args[:bt["!Tina:15:in `topLevelBinding'" Cart.aspects +758 #-> slot +2204 #-> start {runStandard} +17 "valve0"D0% +9 "core" İ%8Z9O= +5 "valve0"D& +8 "carousel0"Fj% +6 "syringe0"B{& +8 "syringe1"C6% +9 Cv& +2 "syringe0"B<% +2 "valve1"E)& +23 "core" ę&&tO; + %2OQ+  + &j t ö%ݕz + &F9 + ¼%jـ + ũ&:O> & +8 ."clearToroid"Toroid.seek :clear +4 "valve1"E>& +7 "valve0"D1( +6 "core" ž&4OB ( + ı( +3 ."detached from Tina"Air.in :default +2 "syringe1"C>d P]z<2 +6 ."detached from Tina"Air.seek :clear +3 "syringe1"C' +5 "core" Ú' +4 ."detached from Tina"Lysis1.in :default +2 "syringe1"C?d P[<2 +7 ."detached from Tina"Lysis1.seek :clear +2 "syringe1"CK( +6 "core" ( +4 ."detached from Tina"Lysis2.in :default + "syringe0"B>d P\\k<2 +6 ."detached from Tina"Lysis2.seek :clear +4 "syringe0"B'j +5 "core" ‘' +3 ."detached from Tina"Archive.in :default +2 "syringe0"B?d PZ<2 +7 ."detached from Tina"Archive.seek :clear +2 "syringe0"B&(j +5 "core" ¦( +3 ."detached from Tina"Carousel.in :default +2 "carousel0"F><` F 22 +6 Fk% +5 "core" %2N4Q  +3 ."detached from Tina"Carousel.home.seek 53 +2 "carousel0"F'2 +17 "core" Ǝ' +2 "valve0"DI% +4 "core" %8Z/OG +4 #"detached from Tina"Selecting Cartridge 53 + "valve0"D?% +8 "core" Ŀ%8ZOB +2 "syringe1"C & +3 "core" Ì&Dp +4 "syringe1"CH% +4 "core" %ݙf +3 "valve1"E2& +3 "core" Ų&+OB ! +5 ."detached from Tina"Carousel.seek 53 +6 "carousel0"FE'2 +17 "core" ' +7 "carousel0"FW% +4 "core" %2NbQ  +3 ."detached from Tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C& +3 "core" Õ&En +5 ."detached from Tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from Tina"Lysis1.seek :locked +2 "syringe1"C( +251 "core" Â( +2 "valve0"D`% +8 "core" %8Z OW +3 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +3 "syringe1"C% +5 "valve0"D9'Z +6 "core" Ò%ݑp +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +8 ."Air"Air.seek :locked +2 "syringe1"C'6 +76 "core" Ĺ' +29 Ð' +2 ."detached from Tina"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F!% +3 "core" ơ%2S\\P  +8 #"detached from Tina"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from Tina"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from Tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from Tina"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" my +4 "cartridge" yAA +6 "Cartridge" Ny  +6 "cartridge" y a"&0J59949 Y%&0lC/q +4 #"detached from Tina"Mounted lysis Cartridge board#J59949 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"D% +5 "core" ē%ZG/O6 - +3 "syringe1"Cl& +5 "core" &n +4 "syringe1"Cb% +4 "core" %6o +2 "carousel0"Fr% +5 "core" %2P \ +4 "carousel0"F% +48 "core" Ɩ%2P \ +164 ."PROCESSING"SPR.command "read_sequences" +24 #"Tina"-> exit +3 #Session Ended +181 !"PROCESSING"o: AnalyticModule::ReadTimeout: mesg"-No response from SPR on AM1 (try 1 of 3):bt["1/home/esp/esp2/lib/delay.rb:105:in `process'"6/home/esp/esp2/lib/schedule.rb:185:in `asyncStep'"1/home/esp/esp2/lib/schedule.rb:473:in `step'"4/home/esp/esp2/lib/schedule.rb:211:in `process'"2/home/esp/esp2/lib/schedule.rb:229:in `start'"1/home/esp/esp2/lib/schedule.rb:228:in `loop'"2/home/esp/esp2/lib/schedule.rb:228:in `start'"-/home/esp/esp2/lib/esp.rb:226:in `shell'"4/home/esp/esp2/lib/realtime.rb:21:in `realTime'"#/home/esp/esp2/mode/real.rb:11"2/home/esp/esp2/lib/framework.rb:110:in `load'"0/home/esp/esp2/lib/framework.rb:110:in `ld'"4/home/esp/esp2/lib/framework.rb:135:in `ldMode'""/home/esp/esp2/lib/esp.rb:371"1/home/esp/esp2/lib/esp.rb:79:in `mainThread'"1/home/esp/esp2/lib/esp.rb:79:in `initialize'"!/home/esp/esp2/lib/esp.rb:79 +358 !o: AnalyticModule::ReadTimeout: mesg"-No response from SPR on AM1 (try 2 of 3):bt["1/home/esp/esp2/lib/delay.rb:105:in `process'"6/home/esp/esp2/lib/schedule.rb:185:in `asyncStep'"1/home/esp/esp2/lib/schedule.rb:473:in `step'"4/home/esp/esp2/lib/schedule.rb:211:in `process'"2/home/esp/esp2/lib/schedule.rb:229:in `start'"1/home/esp/esp2/lib/schedule.rb:228:in `loop'"2/home/esp/esp2/lib/schedule.rb:228:in `start'"-/home/esp/esp2/lib/esp.rb:226:in `shell'"4/home/esp/esp2/lib/realtime.rb:21:in `realTime'"#/home/esp/esp2/mode/real.rb:11"2/home/esp/esp2/lib/framework.rb:110:in `load'"0/home/esp/esp2/lib/framework.rb:110:in `ld'"4/home/esp/esp2/lib/framework.rb:135:in `ldMode'""/home/esp/esp2/lib/esp.rb:371"1/home/esp/esp2/lib/esp.rb:79:in `mainThread'"1/home/esp/esp2/lib/esp.rb:79:in `initialize'"!/home/esp/esp2/lib/esp.rb:79 +359 !o: AnalyticModule::ReadTimeout: mesg" No response from SPR on AM1:@timestampu: Time ϛ8Qm:bt["1/home/esp/esp2/lib/delay.rb:105:in `process'"6/home/esp/esp2/lib/schedule.rb:185:in `asyncStep'"1/home/esp/esp2/lib/schedule.rb:473:in `step'"4/home/esp/esp2/lib/schedule.rb:211:in `process'"2/home/esp/esp2/lib/schedule.rb:229:in `start'"1/home/esp/esp2/lib/schedule.rb:228:in `loop'"2/home/esp/esp2/lib/schedule.rb:228:in `start'"-/home/esp/esp2/lib/esp.rb:226:in `shell'"4/home/esp/esp2/lib/realtime.rb:21:in `realTime'"#/home/esp/esp2/mode/real.rb:11"2/home/esp/esp2/lib/framework.rb:110:in `load'"0/home/esp/esp2/lib/framework.rb:110:in `ld'"4/home/esp/esp2/lib/framework.rb:135:in `ldMode'""/home/esp/esp2/lib/esp.rb:371"1/home/esp/esp2/lib/esp.rb:79:in `mainThread'"1/home/esp/esp2/lib/esp.rb:79:in `initialize'"!/home/esp/esp2/lib/esp.rb:79 +5 !"detached from Tina"o:Thread::ChildDied : mesg":PROCESSING:@timestampu: Time ϛ8Qm: @causeo: AnalyticModule::ReadTimeout;@;" No response from SPR on AM1:bt["1/home/esp/esp2/lib/delay.rb:105:in `process'"6/home/esp/esp2/lib/schedule.rb:185:in `asyncStep'"1/home/esp/esp2/lib/schedule.rb:473:in `step'"4/home/esp/esp2/lib/schedule.rb:211:in `process'"2/home/esp/esp2/lib/schedule.rb:229:in `start'"1/home/esp/esp2/lib/schedule.rb:228:in `loop'"2/home/esp/esp2/lib/schedule.rb:228:in `start'"-/home/esp/esp2/lib/esp.rb:226:in `shell'"4/home/esp/esp2/lib/realtime.rb:21:in `realTime'"#/home/esp/esp2/mode/real.rb:11"2/home/esp/esp2/lib/framework.rb:110:in `load'"0/home/esp/esp2/lib/framework.rb:110:in `ld'"4/home/esp/esp2/lib/framework.rb:135:in `ldMode'""/home/esp/esp2/lib/esp.rb:371"1/home/esp/esp2/lib/esp.rb:79:in `mainThread'"1/home/esp/esp2/lib/esp.rb:79:in `initialize'"!/home/esp/esp2/lib/esp.rb:79:@checkpoint: omitted:@threadIdi sPR +175 #-> SPR +836 #-> slot +106 "Can"k_h+zuNi? +2532 #"preston"-> slot 53=>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +2135 #-> Thread["detached from Tina].abort +3 !o:SyntaxError: mesg"compile error\ preston:5: unterminated string meets end of file\ preston:5: syntax error, unexpected $end, expecting ']'\ Thread["detached from Tina].abort\ ^:@timestampu: Time ϛCs:bt["#preston:5:in `topLevelBinding'" Thread["detached from Tina"].abort +5 !"detached from Tina"o:Thread::Aborted : mesg""Aborted by preston": @cause"detached from Tina:@threadIdiQ:bt["0/home/esp/esp2/lib/thread.rb:482:in `abort'"#preston:6:in `topLevelBinding'" Thread["detached from Tina"].abort +5 !"detached from Tina"o:Thread::Aborted : mesg""Aborted by preston": @cause"detached from Tina:@threadIdiQ:bt["0/home/esp/esp2/lib/thread.rb:482:in `abort'"#preston:7:in `topLevelBinding'" MainThread.exit +4 "sampler0"H% +4 "core" ȏ%%J +6 ."closeHandoff"Handoff.select :close +8 "valve0"D0 +59 #mEXIT +4 #"preston"Session Ended @1785425380.68EDT @1785425391.10EDT = =tMAIN =mMAIN_scheduler @EDT1785425392.35 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +150 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +28 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +57 "Can"q_}+zu] ł@ +366 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +68 #Sea pressure (Abs) tare is 6% of full scale +325 "Can"q_}+zuGŰ@ + q_}+zuGŰ@ + #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"n_+zu\\ų@  +24 #"Tina"Session Begun +762 #-> slot +751 #-> Cart.aspects +1468 #-> start {runStandard} +39 ."detached from Tina"Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +5 .Gate.power :analytic1,:ON,limit to 4000mA +55 "valve0"DP +5 D +3 "core" ā +3 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +10 "valve0"D% +4 "core" Ă%ZG7O7 % +2 ."detached from Tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +6 ."detached from Tina"Plunge.hold +2 "valve0"D+ +6 "core" ă+ +3 ."detached from Tina"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 "sampler0"H% +3 "core" Ȅ%3U +3 ."detached from Tina"SamplePump.configure :default +3 "sampler0"H#^ #(22 +11 "valve0"D& +3 "core" ą&,O4 +2 ."detached from Tina"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from Tina"Toroid.hold +3 "valve0"D, +5 "core" Ć, +3 ."detached from Tina"Toroid.configure :default + "valve0"D$ ֜2 +9 "carousel0"F% +3 "core" Ƈ%2P{ \ +3 ."detached from Tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +10 ."detached from Tina"Carousel.hold +3 "carousel0"F+ +6 "core" ƈ+ +2 ."detached from Tina"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 "ambient0"J % +3 "core" ʉ%] +3 ."detached from Tina"Abs.configure :default +2 "ambient0"J#  +10 "carousel1"G\ & +3 "core" NJ&MQ@ +3 ."detached from Tina"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B & +4 "core" ‹&j +3 ."detached from Tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."detached from Tina"Archive.hold +3 "syringe0"B , +5 "core" Œ, +3 ."detached from Tina"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 "syringe1"C % +3 "core" Í%6P +3 ."detached from Tina"Air.configure :default +3 "syringe1"C#d P]z<2 +6 ."detached from Tina"Air.hold +2 "syringe1"C+ +5 "core" Î+ +3 ."detached from Tina"Air.configure :default + "syringe1"C#d P]z<2 +7 C& +3 "core" Ï&H +3 ."detached from Tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from Tina"Lysis1.hold + "syringe1"C, +5 "core" Ð, +3 ."detached from Tina"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B% +4 "core" ‘%jمJ +2 ."detached from Tina"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +6 ."detached from Tina"Lysis2.hold +2 "syringe0"B+ +4 "core" ’+ +3 ."detached from Tina"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +10 "valve1"E& +5 "core" œ&O/ ) +3 ."detached from Tina"PinPort.configure :default +2 "valve1"E$ B22 +7 ."detached from Tina"PinPort.hold +2 "valve1"E, +5 "core" Ŕ, +3 ."detached from Tina"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from Tina"All elf controllers running firmware version 4.94 +6 "valve0"D% +3 D& +6 "carousel0"F% +8 "core" ĕ%ZGDO,  + Ė&2O' +5 "syringe1"C% +7 "syringe0"B& +5 "syringe1"C& +6 "syringe0"B% +2 "valve1"E& +19 "core" Ɨ%2NQ*  + Ù%6 + ˜&j\ + Ú& + ›%jٕJ + Ŝ&O+ ( +7 ."clearToroid"Toroid.seek :clear +4 "valve0"D( +2 "valve1"E& +10 "core" ĝ( + Ş&O"  +3 ."detached from Tina"Air.seek :clear +3 "syringe1"C' +243 "core" ß' +3 ."detached from Tina"Lysis1.seek :clear +3 "syringe1"C ( +402 "core" à( +3 ."detached from Tina"Lysis2.seek :clear +4 "syringe0"B!'j +9 "core" ¡' +3 ."detached from Tina"Archive.seek :clear +2 "syringe0"B"(j +7 "core" ¢( +2 ."detached from Tina"Carousel.in :default +2 "carousel0"F><` F 22 +6 F#% +5 "core" ƣ%2NQ  +3 ."detached from Tina"Carousel.home.seek 53 +4 "carousel0"F$'2 +17 "core" Ƥ' +2 "valve0"D%% +5 "core" ĥ%ZG0O, , +2 "syringe1"C&& +6 "core" æ&Sv +5 #"detached from Tina"Selecting Cartridge 53 +2 "valve0"D'% +7 "core" ħ%ZG4O* ( +42 ."detached from Tina"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E(& +6 "core" Ũ&O( % +3 ."detached from Tina"Carousel.in :default + "carousel0"F><` F 22 +7 F)% +3 "core" Ʃ%2OQ0  +4 ."detached from Tina"Carousel.home.seek 53 +3 "carousel0"F*'2 +25 "core" ƪ' +2 "carousel0"F+% +5 "core" ƫ%2OQ9  +3 "syringe0"B,& +7 "core" ¬&jt +4 "syringe1"C-% +4 "core" í%ݕs +3 "syringe0"B.% +8 "core" ®%jيt! +5 "syringe1"C/& +5 "core" ï&Gs\ +3 "valve0"D0% +5 "core" İ%ZG0O$ , +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +2 "valve0"D1'8 +104 "core" ı' +3 ."detached from Tina"Carousel.seek 53 +6 "carousel0"F2'2 +14 "core" Ʋ' +5 "carousel0"F3% +4 "core" Ƴ%2NQ3  +2 ."detached from Tina"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +10 "syringe1"C4& +6 "core" ô&Ht +3 ."detached from Tina"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from Tina"Lysis1.seek :locked +4 "syringe1"C5( +248 "core" õ( +3 "valve0"D6% +8 "core" Ķ%8ZOY +2 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +5 "syringe1"C7% +5 ."Plunge"Plunge.seek :locked +5 "valve0"D8'Z +5 "core" ÷%ݘt +5 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C9'6 +77 "core" ĸ' +27 ù' +3 ."detached from Tina"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 F:% +3 "core" ƺ%2SdQ  +7 #"detached from Tina"Powering cartridge bus +263 "cartridge_broadcast" ( +103 ."detached from Tina"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from Tina"PuckHeater.configure :default +2 "Cartridge" \ :D H  +9 ."detached from Tina"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" ;t +5 "cartridge" t +3 "Cartridge" % +6 "core" ľ%ZG7O7 % +3 "syringe1"C?& +5 "core" ÿ&o +2 "syringe1"C@% +5 "core" %6p +2 "carousel0"FA% +5 "core" %2P| \  +3 "carousel0"FB% +6 "core" %2P~ \ +117 ."PROCESSING"SPR.command "read_sequences" +83 .SPR.command "stats_on" +9 "carousel0"FC% +4 "core" %2Pv \  +5 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26jul3011h#2.spr.bz2 +197 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CD% +7 "core" %6d x +44 "syringe1"CE% +3 "core" %6c +47 "syringe1"CF% +3 "core" %6b  +47 "syringe1"CG% +3 "core" %6d +47 "syringe1"CH% +4 "core" %6h +46 "syringe1"CI% +3 "core" %6i | +47 "syringe1"CJ% +3 "core" %6e z +47 "syringe1"CK% +3 "core" %6f ~ +48 "syringe1"CL% +3 "core" %6f +46 "syringe1"CM% +4 "core" %6g z +46 "syringe1"CN% +4 "core" %6f +46 "syringe1"CO% +6 "core" %6b +44 "syringe1"CP% +4 "core" %6` } +48 "syringe1"CQ% +3 "core" %6i +5 "Can"n_+zu0fǪ@ +3 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking +5 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +2 "valve1"ER( N +81 "core" ( +4 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +10 ."PROCESSING"Lysis1.seek 950uL +5 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +6 "syringe1"CS( +52 "syringe0"BT( +12 ."posLyS2"Lysis2.seek 950uL +10 "syringe0"BU' +352 "core" ( +155 ' +322 ( +3 ."PROCESSING"delayUntil "11:41:02.83" +4170 "Can"l_=,zuHɕ@* +585 #"Tina"-> exit +3 #Session Ended +11382 "Can"l_s,zuF̠@H +11972 l^,ztuG*Ϭ@f +11970 l_,zsuMҷ@ +11972 l_>,zuBO@ +6139 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +3 BV% +3 "core" %z. +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 CW& +3 "core" &z2 +48 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 700uL +5 "syringe0"BX'| + "syringe1"CY(| +3486 "core" ( +2 ' +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +3 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +139 #SPR stabilized +3 "syringe1"CZ& +3 "core" &|ev +3 "syringe0"B[% +6 "core" %|e/ +2 ."PROCESSING"delay 20 seconds +1554 "Can"m^,/zu:C5@ +449 ."PROCESSING"SPR.command "start 2 0" +265 #Waiting up to 11:00 for SPR to enter state 1 +11255 "Can"l^,5zzu8ސ@ +511 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B\\% +3 "core" %|e +6 "syringe1"C]& +3 "core" &|ek +45 ."Lysis2mixer"Lysis2.seek 400uL +4 ."PROCESSING"Lysis1.seek 400uL +4 "syringe0"B^' +3 "syringe1"C_( +4183 "core" ' +6 ( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +343 #Waiting up to 1:00:00 for SPR to enter state 7 +5157 "Can"k^Q,Czu5@ +11967 l^,Fzu4 A +11970 l^%,Kzu8}A8 +11968 l^,Lzu4AV +11971 j^3,Izu55At +11970 m^,C +11966 l`,zsu3AC +11968 m`-,zu#MED +11970 k`T,zqu#.ID& +11969 n`a,zsu0MDD +11971 j`p,zru#QDb +11973 l`n,zru,U|D +11971 l`[,zu#Y4D +11969 j`b,zqu1]D +11969 m`N,zsu39`D +6049 "syringe1"Cf& +3 "core" &?Nk  +4 "syringe0"Bg% +4 "core" %?% +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +3595 "Can"l`,zu4cD +8171 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Bh% +3 "core" %? +5 "syringe1"Ci& +9 "core" &?L  +49 ."Lysis2mixer"Lysis2.seek 100uL +6 ."PROCESSING"Lysis1.seek 100uL +2 "syringe0"Bj'ށ +6 "syringe1"Ck(ށ +2090 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1480 "Can"l`,zst-inE +22 ."PROCESSING"SPR.command "hold_off" +356 #Waiting up to 1:00:00 for SPR to enter state 7 +11587 "Can"l`,zuu5mE4 +11967 n`,zru3 q1ER +11970 na4,zsu0tEp +11971 la\ ,!zlu3xE +11968 l`,+zsu5{oE +11965 j`,.zqu4E +11966 n`,0zru3CnE +11968 m`,3zu0PF +11970 l`,3zsu#F$ +11969 k`,1z}u#FB +11968 n`,/zwu1F` +11968 k`,4zqu#OF~ +11971 l`l,:zru0twF +11966 l`m,9zsu#YF +11967 l`,6zqu0 F +11967 la,&zqu6yF +2964 #"PROCESSING"SPR analysis completed +3 !"trapHandler"o:\ fatal: mesg"!Thread(0xb6923578): deadlock:bt["7/home/esp/esp2/lib/schedule.rb:557:in `mainThread'""/home/esp/esp2/lib/esp.rb:371 @1785432494.23EDT @1785434099.65EDT = =tMAIN =mMAIN_scheduler @EDT1785434100.85 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +152 #"core_reset"Sent BREAK +2 #t>BREAK< ESP3 Gateway v1.44 +28 #>BREAK< ESP3 Gateway v1.44 for core bus +2 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +35 "Can"o`V,Mzwu;H +339 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +69 #Sea pressure (Abs) tare is 6% of full scale +333 "Can"o`V,Mzwu0H + o`V,Mzwu0H + #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"k`Q,Fzsu7B4H +85 #"preston"Session Begun +543 #-> ESP.ready! +21 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +56 "valve0"DP +3 D +2 "core" ā +7 "valve0"D% +3 "core" Ă%ZGDO:  +2 ."preston"Plunge.configure :default +3 "valve0"D# P ޤ-2 +7 ."preston"Plunge.hold + "valve0"D+ +6 "core" ă+ +3 ."preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 "sampler0"H% +3 "core" Ȅ% +3 ."preston"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" ą&.O4 +3 ."preston"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."preston"Toroid.hold +2 "valve0"D, +6 "core" Ć, +3 ."preston"Toroid.configure :default +2 "valve0"D$ ֜2 +11 "carousel0"F% +6 "core" Ƈ%2P{ \ +4 ."preston"Carousel.configure :default +3 "carousel0"F#<` F 22 +6 ."preston"Carousel.hold +2 "carousel0"F+ +5 "core" ƈ+ +4 ."preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +36 "ambient0"J % +3 "core" ʉ%[ +3 ."preston"Abs.configure :default + "ambient0"J#  +10 "carousel1"G\ & +6 "core" NJ&NQ6 +3 ."preston"PhaseSeparator.configure :default + "carousel1"G$ +10 "syringe0"B & +3 "core" ‹&)C +2 ."preston"Archive.configure :default +3 "syringe0"B$d PZ<2 +6 ."preston"Archive.hold +3 "syringe0"B , +5 "core" Œ, +3 ."preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 "syringe1"C % +3 "core" Í%6؁ v +3 ."preston"Air.configure :default +2 "syringe1"C#d P]z<2 +7 ."preston"Air.hold +2 "syringe1"C+ +5 "core" Î+ +3 ."preston"Air.configure :default +2 "syringe1"C#d P]z<2 +6 C& +3 "core" Ï&ށ2 +3 ."preston"Lysis1.configure :default +5 "syringe1"C$d P[<2 +8 ."preston"Lysis1.hold + "syringe1"C, +6 "core" Ð, +6 ."preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +34 "syringe0"B% +4 "core" ‘%ށ3= { +3 ."preston"Lysis2.configure :default + "syringe0"B#d P\\k<2 +7 ."preston"Lysis2.hold +3 "syringe0"B+ +5 "core" ’+ +3 ."preston"Lysis2.configure :default + "syringe0"B#d P\\k<2 +13 "valve1"E& +4 "core" œ& N;jO3 4 +2 ."preston"PinPort.configure :default +3 "valve1"E$ B22 +6 ."preston"PinPort.hold +3 "valve1"E, +5 "core" Ŕ, +3 ."preston"PinPort.configure :default +2 "valve1"E$ B22 +6 #"preston"All elf controllers running firmware version 4.94 +76 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !"preston"o:AnalyticModule::Unknown: mesg"SPR:bt["D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"#preston:1:in `topLevelBinding'" ESP.reready! +6 ."Plunge_home"Plunge.in :default +6 ."Toroid_home"Toroid.in :default +6 "valve0"D> P ޤ-2 +5 D? ֜2 +4 ."Carousel_home"Carousel.in :default +10 ."Archive_home"Archive.in :default +8 "carousel0"F><` F 22 +5 ."Air_home"Air.in :default +13 "syringe0"B?d PZ<2 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis1_home"Lysis1.in :default +53 ."Lysis2_home"Lysis2.in :default +5 ."Plunge_home"Plunge.home.jog 0 +5 "syringe1"C>d P]z<2 +7 ."Carousel_home"Carousel.home.jog 0 +3 ."PinPort_home"PinPort.in :default +5 "valve0"D$* +5 ."Archive_home"Archive.home.jog 0 +4 "syringe1"C?d P[<2 +5 "syringe0"B>d P\\k<2 +2 "valve0"D%) +2 ."Air_home"Air.home.jog 0 +7 "carousel0"F&) +6 "valve1"E? B22 +4 "core" Ĥ* +6 "syringe0"B'* +3 "syringe1"C() +2 "valve0"D)& +17 ."PinPort_home"PinPort.home.jog 0 +12 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +9 "valve1"E** +6 "syringe1"C+* +4 "syringe0"B,) +13 "core" ĥ) + ĩ&&+ (2 +4 "syringe1"C> -2 +9 "valve0"D> (2 +2 ."PinPort_home"PinPort.jog 3150 +9 ."Lysis1_home"Lysis1.jog 1602 +5 ."Archive_home"Archive.jog 2841 +4 ."Lysis2_home"Lysis2.jog 1621 +7 ."Air_home"Air.jog 1429 +3 ."Plunge_home"Plunge.jog 1478 +6 "valve1"E4* N +2 "syringe1"C5*B +3 "syringe0"B6*  +10 B7)U +3 "syringe1"C8) +2 "valve0"D9) +109 "core" õ* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +12 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"C:( +5 "core" ø) + ·) + Ĺ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +7 ."Air_home"Air.setRawPosition! 0 +5 ."Plunge_home"Plunge.setRawPosition! 50 +5 "syringe0"B72 +4 "syringe1"C7 +5 "valve0"D72 +4 ."Lysis2_home"Lysis2.seek :home +4 ."Air_home"Air.seek :home +8 "syringe0"B;' +5 "syringe1"C<' +2 ."Plunge_home"Plunge.seek :home +14 "valve0"D=' +3 "core" ú( + ¶* + ü' +3 ."Air_home"Air.in :default +7 ."Archive_home"Archive.setRawPosition! 50 +4 ."Lysis1_home"Lysis1.in :default +5 "syringe1"C>d P]z<2 +2 "syringe0"B82 +2 "syringe1"C?d P[<2 +13 ."Archive_home"Archive.seek :home +3 "syringe0"B>( +7 "core" »' + Ľ' +3 ."Lysis2_home"Lysis2.in :default +3 ."Plunge_home"Plunge.in :default +4 "syringe0"B>d P\\k<2 +3 "valve0"D> P ޤ-2 +9 "core" ¾( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +85 "core" ij* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +3 "valve0"D?( +5 "core" Ŀ( +3 ."Toroid_home"Toroid.in :default +3 "valve0"D? ֜2 +29 "core" Ŵ* + ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +6 ."PinPort_home"PinPort.seek :home +3 "valve1"E@( +433 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +5 "valve1"EA& +4 "valve0"DB( +7 "core" &IOL +8 ."preston"PinPort.in :unlocking + "valve1"E? B2 +7 ."preston"PinPort.seek :clear +2 "valve1"EC( +43 "core" ( +294 ( +3 ."preston"Air.seek :clear +5 "syringe1"CD' +6 "core" ' +3 ."preston"Lysis1.seek :clear +3 "syringe1"CE( +69 "core" ( +4 ."preston"Lysis2.seek :clear +6 "syringe0"BF'j +73 "core" ' +3 ."preston"Archive.seek :clear +3 "syringe0"BG(j +75 "core" ( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 FH% +3 "core" %2RQ  +4 ."preston"Carousel.home.seek 53 +3 "carousel0"FI'2 +16 "core" ' +2 "valve0"DJ% +5 "core" %^O4 !L +2 "valve0"DK% +4 "core" %^O6 !o +3 "sampler0"HL% +5 "core" %) +3 "valve0"DM& +3 "core" &O3 . +4 "carousel0"FN% +4 "core" %2OQ)  +3 "ambient0"JO% +3 "core" %l +4 "carousel1"GP& +3 "core" &NQ +3 "syringe0"BQ& +3 "core" &j׷f 3 +3 "syringe1"CR% +5 "core" %ݜ +2 "syringe1"CS& +43 "core" &a +3 "syringe0"BT% +4 "core" %jٛg +3 "valve1"EU& +3 "core" &O3 0 +23 #"preston"-> ESP.shutdown +5 "sampler0"HV% +5 "core" %! +6 ."closeIntake"Intake.close +4 "sampler0"HW/ +6 ."closeExhaust"Exhaust.close +8 ."closeBypass"Bypass.close +6 "sampler0"HX0 +3 "sampler1"IY/ +6 "core" / + 0 +9 / +9 ."preston"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 .Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +501 #-> exit +2 #Session Ended +2374 "Can"m`,AzquH @1785449010.17EDT = =mMAIN_scheduler =tMAIN @EDT1785449011.24 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +152 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +224 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +367 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +70 #Sea pressure (Abs) tare is 6% of full scale +327 "Can"k`,fzruo + k`,fzruo + #tPolling Can every 2:00 +44 #-> ESP.ready! +5 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +3 "Can"j`,`zbuo +2 .tGate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +16 #Powering core bus +265 "valve0"DP +3 D +3 "core" ā +7 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +7 .tSamplePump.configure :default + "sampler0"H#^ #(22 +7 .tToroid.configure :default +3 "valve0"D$ ֜2 +6 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +7 .tAbs.configure :default +2 "ambient0"J#  +7 .tPhaseSeparator.configure :default + "carousel1"G$ +7 .tArchive.configure :default +3 "syringe0"B$d PZ<2 +7 .tAir.configure :default +3 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 .tPinPort.configure :default +2 "valve1"E$ B22 +31 #tAll elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +322 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !to:AnalyticModule::Unknown: mesg"SPR:bt[ "D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +2 "valve0"D% +8 D& +2 "core" Ă%^zO/ * +2 "carousel0"F% +8 "syringe0"B& +9 ."Plunge_home"Plunge.home.jog 0 +18 "syringe1"C% +5 C& +3 "syringe0"B% +4 "valve1"E & +8 "valve0"D\ ) +13 "core" ă&O, + Ƅ%MP & + …&2t  + Æ%ݦ| + Ç&oN E ˆ%ِw  + ʼn&O+ /l + Ċ) +8 "valve0"D % +12 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +2 ."Archive_home"Archive.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +3 "syringe0"B ) +2 "syringe1"C ) +4 "carousel0"F) +4 "syringe0"B* +3 "valve0"D* +3 "valve1"E* +14 "syringe1"C* +10 "core" ċ%^xO-  + Œ) + Í) + * + Đ* Ǝ) + ő* + Ò* +2 "syringe0"B% +8 "syringe1"C% +7 C& +2 "valve0"D& +2 "valve1"E& +6 ."Plunge_home"Plunge.in :homing +19 "syringe0"B& +2 "valve0"D> (2 +9 ."Plunge_home"Plunge.jog 436 +3 "valve0"D) +3 "core" “%pمs + Õ%ݛ3l  + Ô&ry  + Ė&O  ŗ&[yO  + ˜&w3w| +12 ."Lysis2_home"Lysis2.in :homing +4 ."Lysis1_home"Lysis1.in :homing +3 ."Archive_home"Archive.in :homing +3 ."PinPort_home"PinPort.in :homing +4 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +3 "syringe0"B> (2 +2 "syringe1"C? (2 +3 "syringe0"B? -2 +52 "valve1"E?2 +8 "syringe1"C> -2 +4 "valve0"D?2 +5 ."Lysis2_home"Lysis2.jog 1624 +6 ."Lysis1_home"Lysis1.jog 1597 +4 ."Archive_home"Archive.jog 1615 +3 ."PinPort_home"PinPort.jog 1300 +3 ."Air_home"Air.jog 1428 +4 ."Toroid_home"Toroid.jog 1366 +5 "syringe0"B)X + "syringe1"C*= +2 "syringe0"B*O +7 "valve1"E* +7 "syringe1"C) +3 "valve0"D*V +13 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +4 "valve0"D ' +23 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +3 "valve0"D> P ޤ-2 +54 "core" š) +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +4 "core" Û* + œ* + Þ) +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +5 ."Air_home"Air.setRawPosition! 0 +3 ."Archive_home"Archive.setRawPosition! 50 +3 ."Lysis2_home"Lysis2.seek :home +2 "syringe1"C82 +4 C7 +4 "syringe0"B82 +4 B!' +2 ."Lysis1_home"Lysis1.seek :home +11 ."Air_home"Air.seek :home +4 ."Archive_home"Archive.seek :home +6 "syringe1"C"( +3 C#' +2 "syringe0"B$( +14 "core" ¡' + ŝ* + ã' +4 ."Lysis2_home"Lysis2.in :default +3 ."Air_home"Air.in :default +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "syringe0"B>d P\\k<2 +3 "syringe1"C>d P]z<2 + "valve1"E8 N +11 ."PinPort_home"PinPort.seek :home +3 "valve1"E%( +4 "core" â( + ¤( +3 ."Archive_home"Archive.in :default +3 ."Lysis1_home"Lysis1.in :default +3 "syringe0"B?d PZ<2 +2 "syringe1"C?d P[<2 +213 "core" ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +3 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D&( +3 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +184 "core" ť( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +6 "valve1"E'& +4 "core" ŧ&¬O T +2 "valve0"D(( +12 .tPinPort.in :unlocking +2 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"E)( +40 "core" ũ( +297 Ĩ( +3 .tAir.seek :clear +2 "syringe1"C*' +3 "core" ê' +4 .tLysis1.seek :clear +2 "syringe1"C+( +67 "core" ë( +3 .tLysis2.seek :clear +3 "syringe0"B,'j +73 "core" ¬' +5 .tArchive.seek :clear +2 "syringe0"B-(j +74 "core" ­( +4 .tCarousel.in :default +2 "carousel0"F><` F 22 +5 F.% +3 "core" Ʈ%1UP  +3 .tCarousel.home.seek 52 +3 "carousel0"F/'1 +26 "core" Ư' +2 "valve0"D0% +4 "core" İ%^O> !k +3 "valve0"D1% +4 "core" ı%^O6 !e +4 "sampler0"H2% +4 "core" Ȳ% +4 "valve0"D3& +4 "core" ij&O= +3 "carousel0"F4% +5 "core" ƴ%1NQ  +2 "ambient0"J5% +3 "core" ʵ%j +3 "carousel1"G6& +5 "core" Ƕ&NQ; +2 "syringe0"B7& +5 "core" ·&j-c +3 "syringe1"C8% +4 "core" ø%ݥc +3 "syringe1"C9& +4 "core" ù&]T +3 "syringe0"B:% +5 "core" º%jٞh +2 "valve1"E;& +3 "core" Ż&^O;  +1485 #t-> to 55 +4 "valve0"D<% +3 "core" ļ%^O+ !? +3 "syringe1"C=& +5 "core" ý&W[} +3 "syringe1"C>% +3 "core" þ%ݱYw +3 "valve1"E?& +4 "core" ſ&[O)  +3 .tCarousel.seek 55 +3 "carousel0"F@'4 +84 "core" ' +4 "carousel0"FA% +4 "core" %4)S 2 +2 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"CB& +3 "core" &X] +4 .tLysis1.in :fast + "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +2 "syringe1"CC( +20 "core" ' 4T!P  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 55 + 3 counts: @replyo:I2C::Servo::AbsMove3Reply : @sourceiK: @channeli:@destinationi%: @statuso:I2C::Servo::Status: @inPressi:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni4: @pastRLSF: @pwmi:@deltaPressiP:@pastThresholdF: @voltagef14.901358861070202=: @currentiY: @type2F:@outPressi!:\ @lostF: @error: reseek: @tagiE:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 ' +222 ( +3 "valve0"DD% +7 "core" %^Or !p +3 ."Plunge"Plunge.seek :locked +3 "valve0"DE'Z +6 "syringe1"CF% +6 "core" %ݮ[6 +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +3 "syringe1"CG'6 +82 "core" ' +20 ' +3 .tCarousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 FH% +3 "core" %4]mQ  +6 #tPowering cartridge bus +263 "cartridge_broadcast" ( +3 .tGate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +5 .tPuckHeater.configure :default +42 "Cartridge" \ :D H  +5 .tGate.power :heat,:ON,limit to 5000mA +2 "Cartridge" It +4 "cartridge" t +2 "Cartridge" Jy +15 "cartridge" yAA +2 "Cartridge" Ky  +5 "cartridge" y a"&0J59949 Y%&0lC/q +4 #tMounted lysis Cartridge board#J59949 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6980 "Can"l`,ezfu +11967 l`Z,mzhus +103 #t-> slot +1998 #-> Cart.sampler.prepareLGcart 0,0,0 +3 .Handoff.select :waste +4 "valve0"D +47 #tWaiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CL% +3 "core" %6dY P +47 "syringe1"CM% +5 "core" %6bg K +45 "syringe1"CN% +4 "core" %6af S +46 "syringe1"CO% +4 "core" %6hg W +46 "syringe1"CP% +3 "core" %6jf N +47 "syringe1"CQ% +4 "core" %6cl T +46 "syringe1"CR% +3 "core" %6`q N +47 "syringe1"CS% +3 "core" %6iW M +48 "syringe1"CT% +4 "core" %6 `Z I +46 "syringe1"CU% +4 "core" %6dW J +46 "syringe1"CV% +3 "core" %6aV X +47 "syringe1"CW% +3 "core" %6e< S +47 "syringe1"CX% +5 "core" %6`G Q +45 "syringe1"CY% +3 "core" %6^> R +5 "Can"l`Z,mzhu\  + #tSyringe tare is 50% of full scale at 24C (drifted -0%) +3 .PinPort.in :locking +3 "valve1"E? B22 +3 .tPinPort.seek :locked +3 "valve1"EZ( N +78 "core" ( +4 ."posArchive"Archive.in :fast +13 ."posLyS2"Lysis2.in :fast +2 .tLysis1.seek :empty +5 "syringe0"B?d PZ<2 +4 B>d P\\k<2 + "syringe1"C[( +11 ."posLyS2"Lysis2.seek :empty +6 ."posArchive"Archive.seek :empty +4 "syringe0"B\\' + B]( +671 "core" ( +158 ' +4 ( +2 "syringe0"B^& +5 "core" &)ā> < +8169 "Can"j`,sziu\ tp +11966 j`,gzau\ u +7946 #t-> Archive.to 500; Lysis1.full; Lysis2.full; Archive.empty +3 .Archive.in :default +3 "syringe0"B?d PZ<2 +4 .tArchive.seek 500uL +3 "syringe0"B_(( +478 "core" ( +3 .tLysis1.in :default +2 "syringe1"C?d P[<2 +3 .tLysis1.seek :full +2 "syringe1"C`( +952 .tLysis1.hold +3 "syringe1"Ca, +3 "core" ( zM <- +45 , +3 !to:IRB::Abort: mesg"2Lysis Syringe #1 (LyS1) seek to full Aborted!:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'"./home/esp/esp2/lib/slide.rb:370:in `seek'"0/home/esp/esp2/lib/slide.rb:420:in `moveTo'"8/home/esp/esp2/lib/slide.rb:466:in `method_missing'"(ESP):5:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +1526 #-> Lysis1.empty +5 .Lysis1.seek :empty +4 "syringe1"Cb( +978 "Can"la ,hzot,g  +19 "core" ( +2 "syringe1"Cc& +5 "core" &*"Ϗ <: +1678 #t-> Archive.to 500; Lysis1.full; Lysis2.full; Archive.empty +5 .Archive.seek 500uL +2 "syringe0"Bd(( +3 "core" ( +4 .tLysis1.seek :full +2 "syringe1"Ce( +1653 "core" ( +3 .tLysis2.in :default + "syringe0"B>d P\\k<2 +4 .tLysis2.seek :full +2 "syringe0"Bf' +1653 "core" ' +3 .tArchive.seek :empty +2 "syringe0"Bg( +478 "core" ( +2 "syringe0"Bh& +5 "core" & ) < +5547 #t-> Lysis1.volume +3 "syringe1"Ci& +3 "core" &!W@ h +888 "Can"ka,nzpu\ V#1# +7461 #t-> exit +4 "sampler0"Hj% +3 "core" % +5 ."closeHandoff"Handoff.select :close +4 "valve0"D0 +50 #mEXIT @1785449816.52EDT @1785450983.99EDT = =tMAIN =mMAIN_scheduler @EDT1785450985.04 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +154 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +224 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +370 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +76 #Sea pressure (Abs) tare is 6% of full scale +328 "Can"mj+Xz|u8V + mj+Xz|u8V + #tPolling Can every 2:00 +39 #-> ESP.ready! +5 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +4 "Can"mi+azXu8z +19 #tPowering core bus +266 "valve0"DP +3 D +3 "core" ā +6 .tPlunge.configure :default +3 "valve0"D# P ޤ-2 +6 .tSamplePump.configure :default +2 "sampler0"H#^ #(22 +7 .tToroid.configure :default +2 "valve0"D$ ֜2 +6 .tCarousel.configure :default +3 "carousel0"F#<` F 22 +6 .tAbs.configure :default +2 "ambient0"J#  +7 .tPhaseSeparator.configure :default +2 "carousel1"G$ +7 .tArchive.configure :default +2 "syringe0"B$d PZ<2 +6 .tAir.configure :default +2 "syringe1"C#d P]z<2 +5 .tLysis1.configure :default + "syringe1"C$d P[<2 +5 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 .tPinPort.configure :default +29 "valve1"E$ B22 +5 #tAll elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +318 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !to:AnalyticModule::Unknown: mesg"SPR:bt[ "D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +2 "valve0"D% +4 D& +5 "core" Ă%GMO0 W +4 "carousel0"F% +7 "syringe0"B& +3 ."Plunge_home"Plunge.home.jog 0 +21 "syringe1"C% +3 C& +5 "syringe0"B% +2 "valve1"E\ & +4 "valve0"D ) +20 "core" ă&O$ + Ƅ%M|Q  …&)c  + Æ%d  + Ç&#%  + ˆ%a q Ŋ&;gO.  + ĉ) +13 ."PinPort_home"PinPort.home.jog 0 +4 "valve0"D % + ."Lysis2_home"Lysis2.home.jog 0 +6 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +3 "valve1"E * +4 "syringe0"B ) +2 "syringe1"C) +2 "carousel0"F) +14 "syringe0"B* +5 "valve0"D* + "syringe1"C* +11 "core" ċ%YG@O2  + Ō* +2 ) Î) + * + Ə) đ* + Ò* +4 "valve1"E& +6 "syringe0"B% +13 "syringe1"C& +2 C% +5 "valve0"D& +10 ."Plunge_home"Plunge.in :homing +3 "syringe0"B& +2 "valve0"D> (2 +5 "core" œ& j;pO" +2 ”%ep  + Õ&&g Ö%%h  + ė&O \ + ˜&)cz  +7 ."Plunge_home"Plunge.jog 1475 +8 ."PinPort_home"PinPort.in :homing +7 ."Lysis1_home"Lysis1.in :homing +3 ."Archive_home"Archive.in :homing +4 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +3 ."Lysis2_home"Lysis2.in :homing +4 "valve1"E?2 +2 "valve0"D) +3 "syringe1"C? (2 +8 "syringe0"B? -2 +7 "syringe1"C> -2 +45 "valve0"D?2 +9 "syringe0"B> (2 +2 ."PinPort_home"PinPort.jog 3150 +8 ."Lysis1_home"Lysis1.jog 1447 +4 ."Archive_home"Archive.jog 9650 +4 ."Air_home"Air.jog 1242 +4 ."Toroid_home"Toroid.jog 1368 +3 ."Lysis2_home"Lysis2.jog 1433 +5 "valve1"E* N +2 "syringe1"C* + "syringe0"B*% +10 "syringe1"C) +2 "valve0"D*X +3 "syringe0"B) +15 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +4 "valve0"D ' +23 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +48 "core" Ý) +3 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +2 "core" Û* +5 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 ."Air_home"Air.seek :home +4 "syringe1"C82 +3 C!' +6 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"C"( +3 "core" Ÿ) + á' +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +7 ."Air_home"Air.in :default +2 "syringe0"B72 +2 "syringe1"C>d P]z<2 +8 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B#' +4 "core" â( +4 ."Lysis1_home"Lysis1.in :default +3 "syringe1"C?d P[<2 +13 "core" £' +3 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +227 "core" Ś* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +3 ."PinPort_home"PinPort.seek :home +3 "valve1"E$( +24 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +3 ."Toroid_home"Toroid.seek :home +4 "valve0"D%( +3 "core" ĥ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +25 "core" œ* +2 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +4 ."Archive_home"Archive.seek :home +3 "syringe0"B&( +23 "core" ¦( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +326 "core" Ť( +2 ."PinPort_home"PinPort.in :default +3 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +6 "valve1"E'& +6 "valve0"D(( +6 "core" ŧ&O } +5 .tPinPort.in :unlocking +2 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"E)( +40 "core" ũ( +294 Ĩ( +3 .tAir.seek :clear +3 "syringe1"C*' +4 "core" ê' +3 .tLysis1.seek :clear +3 "syringe1"C+( +67 "core" ë( +2 .tLysis2.seek :clear +4 "syringe0"B,'j +73 "core" ¬' +3 .tArchive.seek :clear +2 "syringe0"B-(j +73 "core" ­( +3 .tCarousel.in :default + "carousel0"F><` F 22 +4 F.% +3 "core" Ʈ%4XP  +4 .tCarousel.home.seek 55 +3 "carousel0"F/'4 +14 "core" Ư' +2 "valve0"D0% +5 "core" İ%^O5 !| +3 "valve0"D1% +4 "core" ı%^O; ! +5 "sampler0"H2% +4 "core" Ȳ% +3 "valve0"D3& +5 "core" ij&O1 % +2 "carousel0"F4% +4 "core" ƴ%4WQ(  +3 "ambient0"J5% +5 "core" ʵ%vc +3 "carousel1"G6& +4 "core" Ƕ&VQ* +3 "syringe0"B7& +3 "core" ·&j.\\} +3 "syringe1"C8% +5 "core" ø%ݯf +2 "syringe1"C9& +5 "core" ù&f%n +2 "syringe0"B:% +5 "core" º%j٩U +2 "valve1"E;& +4 "core" Ż&O5 \ +49 #t-> slot 42=>[:microSPR_phyto, :standard, {lysed:1800, lysed2:1800}] +379 #-> to 42 +3 "valve0"D<% +3 "core" ļ%^ O: ! +3 "syringe1"C=& +4 "core" ý&hLc +3 "syringe1"C>% +3 "core" þ%ݦe +3 "valve1"E?& +4 "core" ſ&O4  +3 .tCarousel.seek 42 +4 "carousel0"F@'( +287 "core" ' +4 "carousel0"FA% +4 "core" %((8S 2 +2 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +5 "syringe1"CB& +3 "core" &_S +3 .tLysis1.in :fast +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +3 "syringe1"CC( +247 "core" ( +3 "valve0"DD% +7 "core" %^sO ! +3 ."Plunge"Plunge.seek :locked +3 "valve0"DE'Z +5 "syringe1"CF% +7 "core" %ݤC +2 ."Air"Air.in :fast +4 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CG'6 +79 "core" ' +23 ' +3 .tCarousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 FH% +3 "core" %(R)CP  +6 #tPowering cartridge bus +305 "cartridge_broadcast" ( +2 .tGate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +15 .tPuckHeater.configure :default +4 "Cartridge" \ :D H  +4 .tGate.power :heat,:ON,limit to 5000mA +4 "Cartridge" It +3 "cartridge" t +2 "Cartridge" Jy +203 "cartridge" y' ' +3 "Cartridge" Ky  +5 "cartridge" y /J59993 /CF +3 #tMounted lysis Cartridge board#J59993 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +623 #-> Cart.unlock +4 .Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +2 "valve1"EL& +3 "core" &OB  +3 .tCarousel.in :default +2 "carousel0"F><` F 22 +5 FM% +2 "core" %()zPf \ +4 .tCarousel.home.seek 42 +3 "carousel0"FN'( +15 "core" ' +2 "carousel0"FO% +5 "core" %(32T 2 +3 "syringe0"BP& +4 "core" &j"m +3 "syringe1"CQ% +4 "core" %6 8 +4 ."Air"Air.in :unlocking +10 "syringe0"BR% + "syringe1"C> P]z-2 +8 "core" %j٤y +4 ."Air"Air.seek :clear +4 "syringe1"CS' +94 "core" ' +2 "syringe1"CT& +5 "core" &k +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"DU% +2 "syringe1"C? P[(2 +6 "core" %ZFO? _ +3 ."Lysis1"Lysis1.seek :clear +8 ."Plunge"Plunge.in :unlocking +3 "syringe1"CV( +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DW'8 +95 "core" ' +24 ( +13 #t-> SC.to 10 +3 .Carousel.seek 10 +3 "carousel0"FX' +675 "core" ' +2 "carousel0"FY% +4 "core" %((oS 2 +2343 #t-> slot 10=> [:microSPR_phyto, :standard, {lysed:1800, lysed2:1800}] +205 #-> to 10 +2 "valve0"DZ% +4 "core" %8ZO2 +3 "syringe1"C[& +4 "core" &WcN +3 "syringe1"C\\% +5 "core" %ݟaT +2 "valve1"E]& +4 "core" & O0  +3 .tCarousel.seek 10 +3 "carousel0"F^' +16 "core" ' +4 "carousel0"F_% +3 "core" %&(aPS 2 +3 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"C`& +6 "core" &_`b +4 .tLysis1.in :fast +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +3 "syringe1"Ca( +247 "core" ( +3 "valve0"Db% +7 "core" %8ZO$ +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +5 ."Plunge"Plunge.seek :locked +5 "syringe1"Cc% +2 "valve0"Dd'Z +9 "core" %ݡ\\y +3 ."Air"Air.in :fast +3 "syringe1"C> P]z<2 +3 ."Air"Air.seek :locked +3 "syringe1"Ce'6 +75 "core" ' +27 ' +3 .tCarousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 Ff% +2 "core" %R(`]P  +8 #tPowering cartridge bus +264 "cartridge_broadcast" ( +2 .tGate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +5 .tPuckHeater.configure :default +3 "Cartridge" \ :D H  +5 .tGate.power :heat,:ON,limit to 5000mA +2 "Cartridge" gy +4 "cartridge" yִjִj +3 "Cartridge" hy  +5 "cartridge" y r/J59943 r/6Cr +3 #tMounted lysis Cartridge board#J59943 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1537 #-> Cart.reserve 10, 42 +145 #-> slots +729 "Can"nf+zluVz +76 #t-> slot 55 =>nil +110 #-> slots +824 #-> to 53 +2 "carousel0"Fi% +4 "core" %(`P \ +3 "valve0"Dj% +4 "core" %ZGO* E +5 .tGate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +3 "valve1"Ek& +3 "core" &O+  +3 .tCarousel.in :default +2 "carousel0"F><` F 22 +6 Fl% +5 "core" %(_Pf \ +3 .tCarousel.home.seek 10 +3 "carousel0"Fm' +14 "core" ' +2 "carousel0"Fn% +5 "core" %M(`Q4  +2 "syringe0"Bo& +4 "core" &j_ +3 "syringe1"Cp% +4 "core" %6u e +5 ."Air"Air.in :unlocking +4 "syringe0"Bq% +5 "syringe1"C> P]z-2 +2 "core" %j٠n +3 ."Air"Air.seek :clear +4 "syringe1"Cr' +93 "core" ' +2 "syringe1"Cs& +5 "core" & āV +3 ."Lysis1"Lysis1.in :unlocking +5 "valve0"Dt% + "syringe1"C? P[(2 +5 "core" %ZGO" B +3 ."Lysis1"Lysis1.seek :clear +13 ."Plunge"Plunge.in :unlocking +3 "syringe1"Cu( +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"Dv'8 +95 "core" ' +22 ( +3 .tCarousel.seek 53 +2 "carousel0"Fw'2 +898 "core" ' +4 "carousel0"Fx% +3 "core" %25 T& 2 +3 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"Cy& +43 "core" &Y}> +3 .tLysis1.in :fast +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +3 "syringe1"Cz( +247 "core" ( +12 "valve0"D{% +9 "core" %8ZO9 +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +4 "syringe1"C|% +5 ."Plunge"Plunge.seek :locked +6 "valve0"D}'Z +5 "core" %ݗ{f +7 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +4 "syringe1"C~'6 +74 "core" ' +28 ' +3 .tCarousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +5 F% +3 "core" %2Y AQ  +6 #tPowering cartridge bus +266 "cartridge_broadcast" ( +3 .tGate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 .tGate.power :cartridge,:OFF +4 #Mounted archive Cartridge +8 !o:Cartridge::Incompatible: mesg"/home/esp/esp2/utils/cartridge/carousel.rb:249:in `mount'"?/home/esp/esp2/utils/cartridge/carousel.rb:327:in `select'":/home/esp/esp2/utils/cartridge/carousel.rb:13:in `to'"(ESP):12:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +238 #-> slots +735 #-> slot 53=>nil +660 #-> slot 55=>[:microSPR_phyto, :standard, {lysed:1800, lysed2:1800}] +273 #-> slots +2078 #-> exit +4 "sampler0"H% +3 "core" ȁ%J +6 #mEXIT @1785451187.67EDT @1785451324.08EDT = =mMAIN_scheduler =tMAIN @EDT1785451325.15 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +152 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +27 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +66 "Can"mdi, zju 7 +311 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +79 #Sea pressure (Abs) tare is 6% of full scale +326 "Can"mdi, zju! + mdi, zju! + #tPolling Can every 2:00 +52 "Can"ld`,z`uD +2076 #t-> ESP.shutdown +5 .Gate.power :analytic1,:OFF +2 .Gate.power :motor,:OFF +2 .Gate.power :core,:OFF +2 .Gate.power :main,:OFF +9874 "Can"mc,zTuD +825 #t-> ESP.ready! +9 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA + .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +18 #Powering core bus +226 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +40 "valve0"DP +3 D +3 "core" ā +6 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +7 .tSamplePump.configure :default + "sampler0"H#^ #(22 +8 .tToroid.configure :default + "valve0"D$ ֜2 +7 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +6 .tAbs.configure :default +2 "ambient0"J#  +7 .tPhaseSeparator.configure :default +2 "carousel1"G$ +7 .tArchive.configure :default +2 "syringe0"B$d PZ<2 +7 .tAir.configure :default +3 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 .tPinPort.configure :default +3 "valve1"E$ B22 +3 #tAll elf controllers running firmware version 4.94 +7 .Gate.power :motor,:ON,limit to 5000mA +5 "valve0"D% +4 D& +3 "core" Ă%GAsO, c +5 "carousel0"F% +5 "syringe0"B& +5 "syringe1"C% +8 ."Plunge_home"Plunge.home.jog 0 +18 "syringe1"C& +6 "syringe0"B% +6 "valve0"D ) +5 "valve1"E\ & +11 "core" ă&O+ + Ƅ%NQ+ ! + …&j  + Æ%)  Ç&h $ + ˆ%٠,{  + Ŋ&*N /q + ĉ) +3 ."Lysis2_home"Lysis2.home.jog 0 +16 "valve0"D % +2 ."Air_home"Air.home.jog 0 +7 ."Carousel_home"Carousel.home.jog 0 +3 ."PinPort_home"PinPort.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +5 "syringe0"B ) + "syringe1"C ) +5 "carousel0"F) +5 "valve1"E* +2 "syringe1"C* +8 "syringe0"B* + "valve0"D* +18 "core" ċ%VGFO1  + Œ) + Í) ŏ* + Ǝ) + Ð* ‘* + Ē* +2 "syringe0"B% +10 "syringe1"C& +3 "syringe0"B& +9 ."Plunge_home"Plunge.in :homing +12 "valve1"E& + "syringe1"C% + "valve0"D& +10 D> (2 +6 "core" “%u٪pQ  + Ô&nW + •&sv  + ×%"oP Ŗ&[O%  + Ę&O$ +7 ."Plunge_home"Plunge.jog 1474 +52 ."Lysis2_home"Lysis2.in :homing +7 ."Toroid_home"Toroid.in :homing +3 ."PinPort_home"PinPort.in :homing +3 ."Archive_home"Archive.in :homing +3 ."Lysis1_home"Lysis1.in :homing +4 ."Air_home"Air.in :homing +3 "valve0"D) +3 "syringe0"B> (2 +2 "valve0"D?2 +4 "valve1"E?2 + "syringe0"B? -2 +9 "syringe1"C? (2 +3 C> -2 +2 ."PinPort_home"PinPort.jog 1301 +11 ."Lysis2_home"Lysis2.jog 1617 +4 ."Toroid_home"Toroid.jog 1364 +4 ."Lysis1_home"Lysis1.jog 2267 +6 ."Archive_home"Archive.jog 1619 +3 ."Air_home"Air.jog 1238 +5 "valve1"E* +2 "syringe0"B)Q +2 "valve0"D*T +7 "syringe1"C* +4 "syringe0"B*S +5 "syringe1"C) +45 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +4 "valve0"D ' +23 "core" Ġ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +24 "core" ›) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B72 +5 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B!' +4 "core" ß) +2 ."Air_home"Air.setRawPosition! 0 +3 "syringe1"C7 +4 ."Air_home"Air.seek :home +4 "syringe1"C"' +6 "core" ž* + â' + ¡' +4 ."Air_home"Air.in :default +3 ."Archive_home"Archive.setRawPosition! 50 +3 ."Lysis2_home"Lysis2.in :default +4 "syringe1"C>d P]z<2 + "syringe0"B82 +3 B>d P\\k<2 +8 ."Archive_home"Archive.seek :home +6 "syringe0"B#( +4 "core" Ý* + Ś* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "syringe1"C82 +2 "valve1"E8 N +11 ."Lysis1_home"Lysis1.seek :home +5 ."PinPort_home"PinPort.seek :home +4 "syringe1"C$( +4 "valve1"E%( +7 "core" £( +4 ."Archive_home"Archive.in :default +3 "syringe0"B?d PZ<2 +8 "core" ä( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +196 "core" Ĝ* + ."Toroid_home"Toroid.setRawPosition! 0 +3 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D&( +4 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +195 "core" ť( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +4 "valve1"E'& +3 "valve0"D(( +4 "core" ŧ&–O) j +6 .tPinPort.in :unlocking +3 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"E)( +40 "core" ũ( +298 Ĩ( +3 .tAir.seek :clear +2 "syringe1"C*' +4 "core" ê' +3 .tLysis1.seek :clear +3 "syringe1"C+( +68 "core" ë( +2 .tLysis2.seek :clear +3 "syringe0"B,'j +73 "core" ¬' +4 .tArchive.seek :clear +3 "syringe0"B-(j +73 "core" ­( +2 .tCarousel.in :default +2 "carousel0"F><` F 22 +5 F.% +3 "core" Ʈ%2TSP  +3 .tCarousel.home.seek 53 +3 "carousel0"F/'2 +15 "core" Ư' +2 "valve0"D0% +5 "core" İ%^~OH ! +3 "valve0"D1% +5 "core" ı%^OG !k +3 "sampler0"H2% +5 "core" Ȳ% +2 "valve0"D3& +5 "core" ij&OJ ' +3 "carousel0"F4% +5 "core" ƴ%2NQ  +2 "ambient0"J5% +4 "core" ʵ%zj +3 "carousel1"G6& +6 "core" Ƕ&NQ& +2 "syringe0"B7& +5 "core" ·&j,Td +3 "syringe1"C8% +5 "core" ø%ݴȁ| +3 "syringe1"C9& +8 "core" ù&iVs +2 "syringe0"B:% +5 "core" º%jٙY] +2 "valve1"E;& +4 "core" Ż&(OF  +1878 #t-> to 42; PhytoSampler.prepareLGcart 0,0,0 +3 "valve0"D<% +3 "core" ļ%^O( !} +3 "syringe1"C=& +5 "core" ý&iK` +2 "syringe1"C>% +4 "core" þ%ݡO\\ +3 "valve1"E?& +3 "core" ſ&3O'  +4 .tCarousel.seek 42 +2 "carousel0"F@'( +246 "core" ' +4 "carousel0"FA% +3 "core" %()EoS 2 +3 .tCarousel.reconfigure :align +3 "carousel0"F#` F 22 +4 "syringe1"CB& +3 "core" &hD[ +7 .tLysis1.in :fast +2 "syringe1"C?d P[<2 +5 .tLysis1.seek :locked +3 "syringe1"CC( +4 "core" ' (ME!Q  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 42 + 3 counts: @replyo:I2C::Servo::AbsMove3Reply : @sourceiK: @channeli:@destinationi%: @statuso:I2C::Servo::Status: @inPressiE:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni(: @pastRLSF: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.038815905743741w: @currentiR: @type2F:@outPressi!:\ @lostF: @error: reseek: @tagiE:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ' +239 ( +3 "valve0"DD% +7 "core" %^} O6 ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"DE'Z +4 "syringe1"CF% +50 "core" %ݯd +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"CG'6 +41 "core" ' +62 ' +2 .tCarousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 FH% +3 "core" %(['Q  +7 #tPowering cartridge bus +265 "cartridge_broadcast" ( +3 .tGate.setIR 1750,5000,0,6 +12 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +15 .tPuckHeater.configure :default +2 "Cartridge" \ :D H  +6 .tGate.power :heat,:ON,limit to 5000mA +3 "Cartridge" It +3 "cartridge" t +2 "Cartridge" Jy +4 "cartridge" y' ' +4 "Cartridge" Ky  +5 "cartridge" y /J59993 /CF +3 #tMounted lysis Cartridge board#J59993 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +4 .Handoff.select :waste +6 "valve0"D +49 #tWaiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CL% +3 "core" %6%pT - +48 "syringe1"CM% +3 "core" %6tN 4 +47 "syringe1"CN% +3 "core" %6#s\\ / +47 "syringe1"CO% +3 "core" %6%tk - +47 "syringe1"CP% +3 "core" %6& , +47 "syringe1"CQ% +4 "core" %6tv 5 +46 "syringe1"CR% +3 "core" %6vt 6 +47 "syringe1"CS% +3 "core" %6!uw 1 +48 "syringe1"CT% +3 "core" %6!r| 1 +47 "syringe1"CU% +4 "core" %6#xe / +46 "syringe1"CV% +3 "core" %6,ud & +47 "syringe1"CW% +3 "core" %6(tb * +47 "syringe1"CX% +3 "core" %6!{W 1 +47 "syringe1"CY% +3 "core" %6vN 7 +4 "Can"mc,zTuF- +2 #tSyringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking + "valve1"E? B22 +5 .tPinPort.seek :locked +2 "valve1"EZ( N +78 "core" ( +4 ."posArchive"Archive.in :fast +9 ."posLyS2"Lysis2.in :fast +2 .tLysis1.seek :empty +2 "syringe0"B?d PZ<2 +4 B>d P\\k<2 +3 "syringe1"C[( +6 ."posLyS2"Lysis2.seek :empty +11 ."posArchive"Archive.seek :empty +7 "syringe0"B\\' + B]( +675 "core" ( +154 ' +3 ( +3 "syringe0"B^& +4 "core" &()M t< +4236 #t-> Archive.to 500; Lysis1.full; Lysis2.full; Archive.empty +4 .Archive.in :default +4 "Can"jc,/znuU3 D + "syringe0"B?d PZ<2 +4 .tArchive.seek 500uL +3 "syringe0"B_(( +478 "core" ( +3 .tLysis1.in :default +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :full +4 "syringe1"C`( +1652 "core" ( +3 .tLysis2.in :default +2 "syringe0"B>d P\\k<2 +4 .tLysis2.seek :full +3 "syringe0"Ba' +1652 "core" ' +3 .tArchive.seek :empty +2 "syringe0"Bb( +478 "core" ( +3 "syringe0"Bc& +4 "core" &)C < +455 #t-> slots +483 #-> to 10 + "carousel0"Fd% +4 "core" %('Pv \  +3 "valve0"De% +4 "core" %ZG#OE 9 +5 .tGate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +5 "valve1"Ef& +4 "core" & N;O>  +3 .tPinPort.in :unlocking +2 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"Eg( +78 "core" ( +2 .tCarousel.in :default +2 "carousel0"F><` F 22 +4 Fh% +3 "core" %(&iPY \  +4 .tCarousel.home.seek 42 +3 "carousel0"Fi'( +16 "core" ' +2 "carousel0"Fj% +5 "core" %(52T 2 +2 "syringe0"Bk& +4 "core" &) +7 ."Archive"Archive.in :unlocking +2 "syringe1"Cl% +2 "syringe0"B? PZ-2 +6 "core" %6kt k +4 ."Archive"Archive.seek :clear +8 ."Air"Air.in :unlocking +4 "syringe0"Bm% +2 Bn(j +2 "syringe1"C> P]z-2 +13 ."Air"Air.seek :clear +4 "syringe1"Co' +6 "core" %\ jq & +6 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +5 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bp'j +73 "core" ' +20 ' +330 ( +3 "syringe1"Cq& +3 "core" &1; X +6 ."Lysis1"Lysis1.in :unlocking +3 "valve0"Dr% +3 "syringe1"C? P[(2 +5 "core" %ZG)O0 3 +3 ."Lysis1"Lysis1.seek :clear +8 ."Plunge"Plunge.in :unlocking +4 "syringe1"Cs( +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +4 "valve0"Dt'8 +83 "core" ( +11 ' +3 .tCarousel.seek 10 +3 "carousel0"Fu' +674 "core" ' +5 "carousel0"Fv% +3 "core" %)(pS 2 +3 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +4 "syringe1"Cw& +3 "core" &aW\\ +4 .tLysis1.in :fast +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +3 "syringe1"Cx( +3 "core" ' N(o!Q  +2 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 10 + 3 counts (sensed 10 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @sourceiK: @channeli:@destinationi%: @statuso:I2C::Servo::Status: @inPressio(:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.004451644575356: @currentiS: @type2F:@outPressi!:\ @lostF: @error: reseek: @tagiz:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +47 ' +242 ( +17 "valve0"Dy% +8 "core" %8ZO8 +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +5 ."Plunge"Plunge.seek :locked +5 "syringe1"Cz% +3 "valve0"D{'Z +6 "core" %ݚX@ +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +3 "syringe1"C|'6 +78 "core" ' +24 ' +3 .tCarousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F}% +3 "core" %Z(aQQ  +9 #tPowering cartridge bus +264 "cartridge_broadcast" ( +3 .tGate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +10 .tPuckHeater.configure :default +3 "Cartridge" \ :D H  +5 .tGate.power :heat,:ON,limit to 5000mA +7 "Cartridge" ~y +4 "cartridge" yִjִj +4 "Cartridge" y  +3 "cartridge" y r/J59943 r/6Cr +4 #tMounted lysis Cartridge board#J59943 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1795 #-> PhytoSampler.prepareLGcart 0,0,0 +3 .Handoff.select :waste +3 #Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C% +4 "core" Á%6F n +47 "syringe1"C% +3 "core" Â%6M m +47 "syringe1"C\ % +3 "core" Ê%6` i +47 "syringe1"C% +3 "core" È%6` w +47 "syringe1"C% +3 "core" Ç%6` k +47 "syringe1"C% +3 "core" Æ%6c z +47 "syringe1"C% +3 "core" Å%6Y o +47 "syringe1"C% +3 "core" Ä%6T v +48 "syringe1"C% +3 "core" Ã%6P q +47 "syringe1"C % +3 "core" Ë%6H j +47 "syringe1"C% +3 "core" Ó%66 q +47 "syringe1"C% +3 "core" Ô%6z" q +47 "syringe1"C% +3 "core" ×%6{) | +47 "syringe1"C% +3 "core" Õ%6ׁ9 } +47 "syringe1"C% +3 "core" Ø%6A s +47 "syringe1"C% +3 "core" Ö%6vL w +47 "syringe1"C % +3 "core" É%6{N z +47 "syringe1"C% +3 "core" Ï%6{X x +47 "syringe1"C % +3 "core" Ì%6xf r +47 "syringe1"C% +3 "core" Ò%6w] n +5 "Can"jc,/znu7. + #tSyringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking + "valve1"E? B22 +5 .tPinPort.seek :locked +3 "valve1"E( N +78 "core" Ő( +4 ."posArchive"Archive.in :fast +10 ."posLyS2"Lysis2.in :fast +4 "syringe0"B?d PZ<2 +3 .tLysis1.seek :empty +6 "syringe0"B>d P\\k<2 +4 "syringe1"C( +4 ."posArchive"Archive.seek :empty +17 ."posLyS2"Lysis2.seek :empty +3 "syringe0"B ( +3 B' +668 "core" Ñ( +161 ( +3 ™' +2 "syringe0"B& +4 "core" ›&D)ހ v< +643 "Can"kb, Archive.to 500; Lysis1.full; Lysis2.full; Archive.empty +4 .Archive.in :default +2 "syringe0"B?d PZ<2 +4 .tArchive.seek 500uL +3 "syringe0"B(( +478 "core" Ÿ( +3 .tLysis1.in :default +2 "syringe1"C?d P[<2 +4 .tLysis1.seek :full +2 "syringe1"C( +1653 "core" Þ( +3 .tLysis2.in :default +2 "syringe0"B>d P\\k<2 +3 .tLysis2.seek :full +3 "syringe0"B' +1652 "core" ' +3 .tArchive.seek :empty +2 "syringe0"B( +479 "core" š( +2 "syringe0"B'& +5 "core" §&%) < +1296 #t-> Cart.unlock +4 .Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +2 "valve1"E& +4 "core" Ŝ& N;O1 +3 .tPinPort.in :unlocking +3 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"E&( +77 "core" Ŧ( +3 .tCarousel.in :default +2 "carousel0"F><` F 22 +3 F%% +3 "core" ƥ%(atPy \ +5 .tCarousel.home.seek 10 +3 "carousel0"F.' +14 "core" Ʈ' +2 "carousel0"F0% +4 "core" ư%W(aQK  +3 "syringe0"B1& +4 "core" ±&)\\2 +5 ."Archive"Archive.in :unlocking +4 "syringe1"C2% +2 "syringe0"B? PZ-2 +7 ."Archive"Archive.seek :clear +3 "syringe0"B3(j +3 "core" ò%6ɺ\\B +6 ."Air"Air.in :unlocking +4 "syringe0"B"% + "syringe1"C> P]z-2 +8 "core" ¢%, 8 +2 ."Air"Air.seek :clear +9 ."Lysis2"Lysis2.in :unlocking +3 "syringe1"C4' + "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +4 "syringe0"B5'j +82 "core" ô' +10 µ' +322 ³( +2 "syringe1"C$& +5 "core" ä&.@ [ +5 ."Lysis1"Lysis1.in :unlocking +4 "valve0"D6% +47 "syringe1"C? P[(2 +6 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C7( +3 "core" Ķ%ZG)OB B +18 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +4 ."Plunge"Plunge.seek :clear +4 "valve0"D8'8 +62 "core" ÷( +33 ĸ' +1242 #t-> SPR.start +115 .SPR.command "read_sequences" +69 .SPR.command "stats_on" +92 ."SPRlogger"SPR.start /var/log/esp/spr26jul3018h.spr.bz2 +911 #t-> SPR.command "start 3 0" +4 .SPR.command "start 3 0" +1463 "Can"mb,Gznu=D +547 #t-> SPR.command "start 2 14" +3 .SPR.command "start 2 14" +471 #-> SPR.command "start 3 0" +2 .SPR.command "start 3 0" +1844 #-> SPR.command "start 2 14" +2 .SPR.command "start 2 14" +2684 #-> SPR.command "start 2 6" +2 .SPR.command "start 2 6" +6417 "Can"mb4,Dzeu>R#D +4087 #t-> SPR.command "start 2 14" +3 .SPR.command "start 2 14" +2855 #-> SPR.command "start 2 9" +2 .SPR.command "start 2 9" +5025 "Can"ja,NzYu<uAD +1754 #t-> SPR.command "start 2 14" +3 .SPR.command "start 2 14" +1474 #-> SPR.command "start 2 7" +3 .SPR.command "start 2 7" +8732 "Can"la,Uzsu"`v_D +6888 #t-> SPR.command "start 2 14" +2 .SPR.command "start 2 14" +1112 #-> SPR.stop +349 #"SPRlogger"Ended log spr26jul3018h.spr.bz2 +2 .tSPR.command "stats_off" +1298 #-> MainThead.exit +3 !o:NameError: mesg"%uninitialized constant MainThead: name:MainThead:bt["(ESP):22:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +705 #-> MainThread.exit +5 "sampler0"H9% +4 "core" ȹ% +5 ."closeHandoff"Handoff.select :close +4 "valve0"D0 +52 #mEXIT @1785452277.33EDT @1785468392.81EDT = =tMAIN =mMAIN_scheduler @EDT1785468393.87 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +155 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +28 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +63 "Can"o^{+zyuM B +303 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +332 "Can"o^{+zyu::D + o^{+zyu::D + #tPolling Can every 2:00 +52 "Can"n^y+zpuO>D +342 #t-> exit +5 #mEXIT @1785468407.47EDT @1785468441.42EDT = =mMAIN_scheduler =tMAIN @EDT1785468442.42 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +146 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +5 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +61 "Can"o^T+z{uLBN +291 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +331 "Can"o^T+z{u9rP + o^T+z{u9rP + #tPolling Can every 2:00 +54 "Can"j^S+zruNvP +14 #t-> slot +7268 #-> start{runStandard} +42 ."detached from MAIN"Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +52 "valve0"DP +3 D +3 "core" ā +7 "valve0"D% +3 "core" Ă%8ZO9 +3 ."detached from MAIN"Plunge.configure :default +5 "valve0"D# P ޤ-2 +4 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +5 ."detached from MAIN"Plunge.hold +3 "valve0"D+ +4 "core" ă+ +2 ."detached from MAIN"Plunge.configure :default +2 "valve0"D# P ޤ-2 +8 "sampler0"H% +3 "core" Ȅ%" +3 ."detached from MAIN"SamplePump.configure :default +3 "sampler0"H#^ #(22 +7 "valve0"D& +3 "core" ą&O1  +3 ."detached from MAIN"Toroid.configure :default +3 "valve0"D$ ֜2 +4 ."detached from MAIN"Toroid.hold +3 "valve0"D, +3 "core" Ć, +3 ."detached from MAIN"Toroid.configure :default +3 "valve0"D$ ֜2 +6 "carousel0"F% +4 "core" Ƈ%Y(auQU  +3 ."detached from MAIN"Carousel.configure :default +3 "carousel0"F#<` F 22 +7 ."detached from MAIN"Carousel.hold +3 "carousel0"F+ +4 "core" ƈ+ +3 ."detached from MAIN"Carousel.configure :default +3 "carousel0"F#<` F 22 +6 "ambient0"J % +4 "core" ʉ%[ +3 ."detached from MAIN"Abs.configure :default +3 "ambient0"J#  +7 "carousel1"G\ & +3 "core" NJ&XtQO +3 ."detached from MAIN"PhaseSeparator.configure :default +2 "carousel1"G$ +7 "syringe0"B & +3 "core" ‹&j3_ +4 ."detached from MAIN"Archive.configure :default +2 "syringe0"B$d PZ<2 +4 ."detached from MAIN"Archive.hold +3 "syringe0"B , +4 "core" Œ, +3 ."detached from MAIN"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 "syringe1"C % +4 "core" Í%ݘ +3 ."detached from MAIN"Air.configure :default +2 "syringe1"C#d P]z<2 +5 ."detached from MAIN"Air.hold +2 "syringe1"C+ +4 "core" Î+ +3 ."detached from MAIN"Air.configure :default +2 "syringe1"C#d P]z<2 +5 C& +3 "core" Ï&R +3 ."detached from MAIN"Lysis1.configure :default +3 "syringe1"C$d P[<2 +45 ."detached from MAIN"Lysis1.hold +4 "syringe1"C, +3 "core" Ð, +2 ."detached from MAIN"Lysis1.configure :default +3 "syringe1"C$d P[<2 +4 "syringe0"B% +4 "core" ‘%jٟ% +6 ."detached from MAIN"Lysis2.configure :default +4 "syringe0"B#d P\\k<2 +7 ."detached from MAIN"Lysis2.hold +2 "syringe0"B+ +4 "core" ’+ +3 ."detached from MAIN"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 "valve1"E& +3 "core" œ&O-  +3 ."detached from MAIN"PinPort.configure :default +2 "valve1"E$ B22 +5 ."detached from MAIN"PinPort.hold +2 "valve1"E, +4 "core" Ŕ, +3 ."detached from MAIN"PinPort.configure :default +2 "valve1"E$ B22 +4 #"detached from MAIN"All elf controllers running firmware version 4.94 +6 "valve0"D% +5 "core" ĕ%8ZO3 +4 "valve0"D& +4 "carousel0"F% +8 "syringe0"B& +5 "syringe1"C% +4 C& +6 "syringe0"B% +4 "valve1"E& +23 "core" Ė&O5  + Ɨ%Y(`QV  + ˜&j4_ + Ù%ݖ Ú&[ + ›%jٗ + Ŝ&O7  +11 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +3 "valve0"D( +9 "core" ŝ&O6  + Ğ( +3 ."detached from MAIN"Air.seek :clear +3 "syringe1"C' +4 "core" ß' +4 ."detached from MAIN"Lysis1.seek :clear +3 "syringe1"C ( +5 "core" à( +3 ."detached from MAIN"Lysis2.seek :clear +3 "syringe0"B!'j +4 "core" ¡' +4 ."detached from MAIN"Archive.seek :clear +3 "syringe0"B"(j +7 "core" ¢( +4 ."detached from MAIN"Carousel.in :default +2 "carousel0"F><` F 22 +5 F#% +3 "core" ƣ%X(a}QL  +4 ."detached from MAIN"Carousel.home.seek 10 +4 "carousel0"F$' +14 "core" Ƥ' +2 "valve0"D%% +4 "core" ĥ%8ZO/ +5 #"detached from MAIN"Selecting Cartridge 55 +2 "valve0"D&% +4 "core" Ħ%8ZO1 +3 "syringe1"C'& +4 "core" ç&\\W +3 "syringe1"C(% +4 "core" è%ݙZ +3 "valve1"E)& +4 "core" ũ&O/  +3 ."detached from MAIN"Carousel.seek 55 +4 "carousel0"F*'4 +938 "core" ƪ' +4 "carousel0"F+% +3 "core" ƫ%4-T 2 +3 ."detached from MAIN"Carousel.reconfigure :align +4 "carousel0"F#` F 22 +3 "syringe1"C,& +4 "core" ì&PQ +4 ."detached from MAIN"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from MAIN"Lysis1.seek :locked +3 "syringe1"C-( +248 "core" í( +2 "valve0"D.% +8 "core" Į%8ZO +3 ."Plunge"Plunge.seek :locked +4 "valve0"D/'Z +5 "syringe1"C0% +7 "core" ð%ݞLm +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +5 ."Air"Air.seek :locked +3 "syringe1"C1'6 +70 "core" į' +32 ñ' +2 ."detached from MAIN"Carousel.reconfigure :locked +3 "carousel0"F#` F 2\ 2 +4 F2% +3 "core" Ʋ%4R[Q  +7 #"detached from MAIN"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from MAIN"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +5 ."detached from MAIN"PuckHeater.configure :default +3 "Cartridge" \ :D H  +5 ."detached from MAIN"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" 3t +3 "cartridge" t +3 "Cartridge" 4y +4 "cartridge" yAA +3 "Cartridge" 5y  +4 "cartridge" y a"&0J59949 Y%&0lC/q +4 #"detached from MAIN"Mounted lysis Cartridge board#J59949 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"D6% +6 "core" Ķ%ZGAO"  +4 "syringe1"C7& +5 "core" ÷&H +3 "syringe1"C8% +4 "core" ø%6I i +2 "carousel0"F9% +4 "core" ƹ%4Pl \  +4 "carousel0"F:% +4 "core" ƺ%4}Pp \  +118 ."PROCESSING"SPR.command "read_sequences" +15 .SPR.command "stats_on" +8 "carousel0"F;% +4 "core" ƻ%4P \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3023h#2.spr.bz2 +252 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +3 "valve0"D +104 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C<% +4 "core" ü%6D _ +46 "syringe1"C=% +3 "core" ý%6@ ] +47 "syringe1"C>% +3 "core" þ%6E f +48 "syringe1"C?% +3 "core" ÿ%6G c +46 "syringe1"C@% +3 "core" %6I \\ +47 "syringe1"CA% +3 "core" %6E _ +47 "syringe1"CB% +4 "core" %6D b +46 "syringe1"CC% +4 "core" %6 i +47 "syringe1"CD% +3 "core" %6 c +47 "syringe1"CE% +6 "core" %6 b +44 "syringe1"CF% +3 "core" %6 b +47 "syringe1"CG% +3 "core" %6 a +47 "syringe1"CH% +5 "core" %6 _ +45 "syringe1"CI% +3 "core" %6 _ +4 "Can"j^S+zru9qlp +2 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +5 .PinPort.in :locking +2 "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +3 "valve1"EJ( N +78 "core" ( +4 ."posArchive"Archive.in :fast +9 ."posLyS2"Lysis2.in :fast +4 ."PROCESSING"Lysis1.seek 1800uL +3 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +2 "syringe1"CK( +16 ."posLyS2"Lysis2.seek 1800uL +4 ."posArchive"Archive.seek :empty +4 "syringe0"BL' +3 BM( +119 "core" ( +84 ' +629 ( +2 ."PROCESSING"delayUntil "23:39:14.28" +12 "Can"k^+ztt<n +11961 m]+zruPy +11967 l]+zquQB +11969 l]+zsuQ7 +11967 m]+zyuOB +10371 ."PROCESSING"Lysis2.in :DAmix +4 "syringe0"B>d2 P\\k<2 +4 BN% +3 "core" % +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +4 CO& +4 "core" & V +50 ."Lysis2mixer"Lysis2.seek 1550uL +8 ."PROCESSING"Lysis1.seek 1550uL +6 "syringe0"BP' +3 "syringe1"CQ( +1504 "Can"l]+zqs] +1983 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +504 .Handoff.select SPR +5 "valve0"D@ +50 ."PROCESSING"SPR.command "start 2 14" +123 #SPR stabilized +3 "syringe1"CR& +3 "core" &Q  +3 "syringe0"BS% +6 "core" %DH +2 ."PROCESSING"delay 20 seconds +2004 .SPR.command "start 2 0" +316 #Waiting up to 11:00 for SPR to enter state 1 +6959 "Can"l]^+zru>ȃ" +4851 ."PROCESSING"SPR.command "hold_on" +136 #SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +4 "syringe0"BT% +3 "core" %2v +3 "syringe1"CU& +8 "core" &Ju  +50 ."Lysis2mixer"Lysis2.seek 1250uL +7 ."PROCESSING"Lysis1.seek 1250uL +3 "syringe0"BV' +3 "syringe1"CW( +4181 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +293 #Waiting up to 1:00:00 for SPR to enter state 7 +914 "Can"k]I+zrt-H@ +11968 k]+zsu90^ +11971 h]+zquB3| +11968 l]+zru<x +11969 k],+zsu=ݼ +11973 j]+zru= +11970 m] +zsu@l +11971 l]+zru> +11968 l]+zsu=0 +890 #"SPRlogger"SPRsummary:5e+09RIU,1e+09RIU,52.2RIU,1e+09RIU after 3707s @EDT1785470486.84 "Can"l\\+zsu"N +4903 #t-> threads +7065 "Can"l\\+zqu!l +11971 l\\_+zsu@) +11972 l[+zru"0 +11967 k[+zru!1 +11970 l\\)+ztu;p +11969 k\\[+zou@ +2798 "syringe1"CX& +4 "core" &S L +3 "syringe0"BY% +5 "core" % +3 ."PROCESSING"delay 20 seconds +2004 .SPR.command "start 2 0" +305 #Waiting up to 11:00 for SPR to enter state 1 +4606 #"brent"Session Begun +672 #-> threads +1568 "Can"l\\R+zru?E +4924 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +5 "syringe0"BZ% +6 "core" %  +5 "syringe1"C[& +6 "core" & L +53 ."Lysis2mixer"Lysis2.seek 1100uL +5 ."PROCESSING"Lysis1.seek 1100uL +8 "syringe0"B\\'" +6 "syringe1"C](" +2095 "core" ' +7 ( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +323 #Waiting up to 1:00:00 for SPR to enter state 7 +2825 "Can"k\\2+zrt-A.> +11969 m\\i+zru:)\\ +11968 k\\+zru=#z +11970 l\\+z~u9 j +11967 k\\]+zru= +11965 l\\P+zut' +11969 l\\3+zru>"d +11968 n\\;+zqu>% +11966 j\\;+zru"P(. +11967 j\\-+zsu",L +11966 k\\?+zlu"0j +11969 l\\.+zou2]4 +11970 j\\n+zru"68U +11970 l]+zou"C +812 "syringe1"C^& +5 "core" &"|` " +4 "syringe0"B_% +5 "core" %""] +2 ."PROCESSING"delay 20 seconds +2005 .SPR.command "start 2 0" +304 #Waiting up to 11:00 for SPR to enter state 1 +8833 "Can"j^5+zqu?FS +2935 ."PROCESSING"SPR.command "hold_on" +136 #SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +5 "syringe0"B`% +6 "core" %"l +4 "syringe1"Ca& +6 "core" &"  +64 ."Lysis2mixer"Lysis2.seek 950uL +8 ."PROCESSING"Lysis1.seek 950uL +8 "syringe0"Bb' +3 "syringe1"Cc( +2094 "core" ' +5 ( +3 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +275 #Waiting up to 1:00:00 for SPR to enter state 7 +4911 "Can"j]+zpt-BL< +11964 m]+zsu=OZ +11963 m]<+zru@S7x +11963 k\\+zqu@V +11967 l\\+zquBY +11964 l\\j+zqu<]7 +11969 n\\+zsu:`} +11967 i\\6+zsu7c +11968 k]m+zcu"Eg:, +11971 k^^+ztu"k;J +11970 k^+zsu"o9h +11969 l^\\+zpu8r +11969 l^ +z\\u"v +11966 l]+zru:Zz +11967 k]A+zpu=} +10809 #"PROCESSING"SPR analysis completed +1159 "Can"l\\+zru;. +97 #"brent"-> threads +1164 #"SPRlogger"Ended log spr26jul3023h#2.spr.bz2 +6 ."PROCESSING"SPR.command "stats_off" +71 "carousel0"Fd% +4 "core" %4rPF \  +2322 #"brent"-> threads +8301 "Can"m\\+zsu?yh +3735 #t-> Syringe +5 "syringe0"Be& +5 "core" &)b +3 "syringe1"Cf% +4 "core" %6]( +3 "syringe1"Cg& +4 "core" &z` { +7 "syringe0"Bh% +4 "core" %{c ` +3364 #t-> Cart.unlock +6 .Gate.power :heat,:OFF +4 .Gate.power :cartridge,:OFF +4 "valve1"Ei& +5 "core" & N;&O" x +4 .tPinPort.in :unlocking +2 "valve1"E? B2 +11 .tPinPort.seek :clear +3 "valve1"Ej( +81 "core" ( +2 .tCarousel.in :default +3 "carousel0"F><` F 22 +7 Fk% +3 "core" %4P \  +4 .tCarousel.home.seek 55 +3 "carousel0"Fl'4 +19 "core" ' +2 "carousel0"Fm% +3 "core" %4"S 2 +3 "syringe0"Bn& +4 "core" &) +5 ."Archive"Archive.in :unlocking +5 "syringe1"Co% +7 "syringe0"B? PZ-2 +4 "core" %6 +3 ."Archive"Archive.seek :clear +9 "syringe0"Bp% +8 ."Air"Air.in :unlocking +4 "syringe0"Bq(j +4 "syringe1"C> P]z-2 +9 ."Air"Air.seek :clear +7 "syringe1"Cr' +6 "core" %{$ W +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +7 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bs'j +71 "core" ' +204 ' +145 ( +3 "syringe1"Ct& +3 "core" &ź +5 ."Lysis1"Lysis1.in :unlocking +4 "valve0"Du% +4 "syringe1"C? P[(2 +9 ."Lysis1"Lysis1.seek :clear +3 "syringe1"Cv( +6 "core" %ZGdO4 A +5 ."Plunge"Plunge.in :unlocking +4 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"Dw'8 +96 "core" ' +156 ( +1278 #t-> slots 55=> [:microSPR_phyto, :standard, {lysed:1100, lysed2: 1100}] +2566 "Can"l\\+zqu2: +2585 #t-> Cart.standard +2186 #-> exit +6 "sampler0"Hx% +4 "core" % +3 ."closeIntake"Intake.close +11 ."closeExhaust"Exhaust.close +11 ."closeBypass"Bypass.close +6 "sampler0"Hy/ +3 ."closeHandoff"Handoff.select :close +8 "sampler0"Hz0 +3 "sampler1"I{/ +6 "valve0"D +12 "core" / + 0 + / +40 #mEXIT +2 #"brent"Session Ended @1785475562.22EDT @1785475821.67EDT = =mMAIN_scheduler =tMAIN @EDT1785475823.47 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +163 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +31 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +37 "Can"l^+zwuQ&2 +344 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +70 #Sea pressure (Abs) tare is 6% of full scale +346 "Can"l^+zwu@' +2 l^+zwu@' #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"k^+zruRJ +61 #"brent"Session Begun +12 #-> ESP::Mode +6918 #-> slots +1939 #-> Cart.aspects +1824 #-> Cart.aspectsOf :standard, extraDilutions:[],lysed:1100, lysed2:1100 +1215 "Can"l^+ztuQJ +921 #"brent"-> start{runStandard} +36 ."detached from brent"Gate.power :sampler,:OFF,limit to 3500mA +8 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +55 "valve0"DP +4 D +2 "core" ā +7 "valve0"D% +3 "core" Ă%8ZO3 +3 ."detached from brent"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from brent"Plunge.hold +2 "valve0"D+ +5 "core" ă+ +3 ."detached from brent"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 "sampler0"H% +3 "core" Ȅ%m +3 ."detached from brent"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" ą&O6 " +5 ."detached from brent"Toroid.configure :default +2 "valve0"D$ ֜2 +5 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +7 ."detached from brent"Toroid.hold +2 "valve0"D, +5 "core" Ć, +3 ."detached from brent"Toroid.configure :default +2 "valve0"D$ ֜2 +11 "carousel0"F% +6 "core" Ƈ%4MQ!  +6 ."detached from brent"Carousel.configure :default +4 "carousel0"F#<` F 22 +11 ."detached from brent"Carousel.hold +4 "carousel0"F+ +8 "core" ƈ+ + ."detached from brent"Carousel.configure :default +3 "carousel0"F#<` F 22 +9 "ambient0"J % +3 "core" ʉ%tW +3 ."detached from brent"Abs.configure :default +2 "ambient0"J#  +9 "carousel1"G\ & +3 "core" NJ&NQ +3 ."detached from brent"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B & +4 "core" ‹&j/ +2 ."detached from brent"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from brent"Archive.hold +2 "syringe0"B , +6 "core" Œ, +3 ."detached from brent"Archive.configure :default + "syringe0"B$d PZ<2 +10 "syringe1"C % +3 "core" Í%ݒ& +3 ."detached from brent"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from brent"Air.hold +2 "syringe1"C+ +5 "core" Î+ +4 ."detached from brent"Air.configure :default + "syringe1"C#d P]z<2 +6 C& +3 "core" Ï&\\E +3 ."detached from brent"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from brent"Lysis1.hold +2 "syringe1"C, +5 "core" Ð, +2 ."detached from brent"Lysis1.configure :default +3 "syringe1"C$d P[<2 +6 "syringe0"B% +3 "core" ‘%jٛB +3 ."detached from brent"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."detached from brent"Lysis2.hold +2 "syringe0"B+ +5 "core" ’+ +4 ."detached from brent"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +12 "valve1"E& +5 "core" œ&O1 h +3 ."detached from brent"PinPort.configure :default +2 "valve1"E$ B22 +7 ."detached from brent"PinPort.hold +2 "valve1"E, +6 "core" Ŕ, +3 ."detached from brent"PinPort.configure :default +2 "valve1"E$ B22 +7 #"detached from brent"All elf controllers running firmware version 4.94 +6 "valve0"D% +4 D& +5 "carousel0"F% +7 "core" ĕ%8[ O/ + Ė&O"  +3 "syringe0"B& +8 "syringe1"C% +2 C& +6 "syringe0"B% +3 "valve1"E& +34 "core" Ɨ%4OQ)  +3 ˜&j/ + Ù%ݔ + Ú&\\ + ›%jٙ51 + Ŝ&O& f +8 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +2 "valve0"D( +10 "core" ŝ&O) p + Ğ( +4 ."detached from brent"Air.seek :clear +2 "syringe1"C' +7 "core" ß' +3 ."detached from brent"Lysis1.seek :clear +3 "syringe1"C ( +7 "core" à( +3 ."detached from brent"Lysis2.seek :clear +2 "syringe0"B!'j +7 "core" ¡' +2 ."detached from brent"Archive.seek :clear +3 "syringe0"B"(j +11 "core" ¢( +2 ."detached from brent"Carousel.in :default +2 "carousel0"F><` F 22 +6 F#% +3 "core" ƣ%4NQ  +4 ."detached from brent"Carousel.home.seek 55 +3 "carousel0"F$'4 +47 "core" Ƥ' +2 "valve0"D%% +5 "core" ĥ%8ZO' +11 #"detached from brent"Selecting Cartridge 55 +2 "valve0"D&% +7 "core" Ħ%8ZO( +3 "syringe1"C'& +5 "core" ç&Y +3 "syringe1"C(% +3 "core" è%ݓ +3 "valve1"E)& +4 "core" ũ&O' f +4 ."detached from brent"Carousel.seek 55 +2 "carousel0"F*'4 +17 "core" ƪ' +4 "carousel0"F+% +4 "core" ƫ%4N;Q7  +3 ."detached from brent"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"C,& +4 "core" ì&]- +3 ."detached from brent"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from brent"Lysis1.seek :locked +3 "syringe1"C-( +250 "core" í( +3 "valve0"D.% +7 "core" Į%8ZO +3 ."Plunge"Plunge.seek :locked +3 "valve0"D/'Z +8 "syringe1"C0% +6 "core" ð%ݍy +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +8 ."Air"Air.seek :locked +2 "syringe1"C1'6 +69 "core" į' +37 ñ' +2 ."detached from brent"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F2% +3 "core" Ʋ%4R}Q  +10 #"detached from brent"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from brent"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from brent"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."detached from brent"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" 3t +3 "cartridge" t +3 "Cartridge" 4y +4 "cartridge" yAA +3 "Cartridge" 5y  +4 "cartridge" y a"&0J59949 Y%&0lC/q +4 #"detached from brent"Mounted lysis Cartridge board#J59949 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"D6% +5 "core" Ķ%ZG6O# & +4 "syringe1"C7& +3 "core" ÷&} +3 "syringe1"C8% +5 "core" ø%6m a +2 "carousel0"F9% +3 "core" ƹ%4Pq \ +4 "carousel0"F:% +6 "core" ƺ%4Pn \ +118 ."PROCESSING"SPR.command "read_sequences" +49 .SPR.command "stats_on" +9 "carousel0"F;% +3 "core" ƻ%4{Pt \ +7 ."PROCESSING"SPR.command "start 0 1" +69 ."SPRlogger"SPR.start /var/log/esp/spr26jul3101h.spr.bz2 +197 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +55 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +4 "syringe1"C<% +12 "core" ü%6͂ i +37 "syringe1"C=% +3 "core" ý%6y l +47 "syringe1"C>% +4 "core" þ%6t j +46 "syringe1"C?% +4 "core" ÿ%6u o +46 "syringe1"C@% +4 "core" %6o k +46 "syringe1"CA% +4 "core" %6u g +46 "syringe1"CB% +4 "core" %6t h +46 "syringe1"CC% +4 "core" %6q f +47 "syringe1"CD% +3 "core" %6i f +47 "syringe1"CE% +3 "core" %6 \ i +47 "syringe1"CF% +4 "core" %6s o +47 "syringe1"CG% +7 "core" %6t n +42 "syringe1"CH% +4 "core" %6l j +47 "syringe1"CI% +3 "core" %6r d +5 "Can"l^+ztu9 + #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +5 .PinPort.in :locking +2 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +5 "valve1"EJ( N +78 "core" ( +4 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +8 ."posLyS2"Lysis2.in :fast +9 ."PROCESSING"Lysis1.seek 1100uL +4 "syringe0"B>d P\\k<2 +3 ."posArchive"Archive.seek :empty +8 "syringe1"CK(" +3 "syringe0"BL( +7 ."posLyS2"Lysis2.seek 1100uL +10 "syringe0"BM'" +289 "core" ( +160 ' +371 ( +2 ."PROCESSING"delayUntil "01:43:03.14" +7279 "Can"l_@+zuuP;J +11972 l^+zvuOZJ +11969 k^>+z|uNJzJ +11972 l]+zwuLq!J +11974 j]h+zsuO"g?J +3093 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +8 BN% +6 "core" %"́ . +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +6 CO& +3 "core" &"q, - +42 ."Lysis2mixer"Lysis2.seek 850uL +9 ."PROCESSING"Lysis1.seek 850uL +3 "syringe0"BP' +3 "syringe1"CQ( +3486 "core" ' +5 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +127 #SPR stabilized +2 "syringe1"CR& +6 "core" &rT R +3 "syringe0"BS% +6 "core" %rJ / +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +317 #Waiting up to 11:00 for SPR to enter state 1 +2277 "Can"m\\+zru<]J +9532 ."PROCESSING"SPR.command "hold_on" +136 #SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BT% +6 "core" %rW 9 +3 "syringe1"CU& +7 "core" &rS* S +47 ."Lysis2mixer"Lysis2.seek 550uL +7 ."PROCESSING"Lysis1.seek 550uL +5 "syringe0"BV' +4 "syringe1"CW( +2223 "Can"i\\+zps1t{J +1962 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +292 #Waiting up to 1:00:00 for SPR to enter state 7 +8212 "Can"j\\4+zgu??J +11969 k\\/+zou?J +11968 l\\+zmu>J +11972 m]+ztu>J +11974 m^+zru>XJ +11968 j^U+zru>/J +11972 l]+zru< MJ +11974 j]+zru=KkJ +5551 #"SPRlogger"SPRsummary:4.89e+09RIU,1e+09RIU,14.7RIU,1e+09RIU after 10801s +6422 "Can"l]d+zsu!J +11974 k] +zpu" J +11973 k\\+zqu"J +11971 k\\+zsu>.sJ +11971 m\\T+zru"2FJ +11971 l\\+zru8JJ +11971 l]+zsu9]=J +7441 #"PROCESSING"SPR analysis completed +324 #"SPRlogger"Ended log spr26jul3101h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +49 "carousel0"FX% +7 "core" %4tPR \ +4149 "Can"j^+ztu>| +1391 #"brent"-> threads +8187 #-> slot +2391 "Can"o^+zsu@fyJ +11972 k^x+zqu@S\ J +3725 #"brent"-> exit +3 #Session Ended +1460 #Session Begun +702 #-> ESP.main.exit +4 "sampler0"HY% +5 "core" %K +4 ."closeIntake"Intake.close +10 ."closeExhaust"Exhaust.close +5 ."closeBypass"Bypass.close +4 ."closeHandoff"Handoff.select :close +7 "sampler0"HZ/ +4 H[0 +2 "sampler1"I\\/ +3 "valve0"D +17 "core" / + 0 + / +42 #mEXIT +5 #"brent"Session Ended @1785479006.64EDT @1785479015.29EDT = =tMAIN =mMAIN_scheduler @EDT1785479016.60 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +142 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +31 "Can"n^\\+zwu]S +335 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +67 #Sea pressure (Abs) tare is 6% of full scale +352 "Can"n^\\+zwuH< + n^\\+zwuH< + #tPolling Can every 2:00 +50 #Clients may connect on TCP port 9999 + #Waiting for espclients... +2 "Can"l^N+zuu[` +983 #"brent"Session Begun +1090 #-> slot +1726 #-> Cart.release 56 +781 #-> Cart.release 42 +373 #-> slot +7020 "Can"k]+zsu^t` +11972 m]+zsu_`W` +3302 #"brent"-> start{runStandard} +41 ."detached from brent"Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +54 "valve0"DP +3 D +3 "core" ā +6 "valve0"D% +3 "core" Ă%ZG6 O7 & +3 ."detached from brent"Plunge.configure :default + "valve0"D# P ޤ-2 +7 ."detached from brent"Plunge.hold +2 "valve0"D+ +5 "core" ă+ +3 ."detached from brent"Plunge.configure :default +2 "valve0"D# P ޤ-2 +11 "sampler0"H% +5 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +3 "core" Ȅ%R +5 ."detached from brent"SamplePump.configure :default +2 "sampler0"H#^ #(22 +10 "valve0"D& +3 "core" ą&O3 +3 ."detached from brent"Toroid.configure :default + "valve0"D$ ֜2 +8 ."detached from brent"Toroid.hold + "valve0"D, +5 "core" Ć, +3 ."detached from brent"Toroid.configure :default +2 "valve0"D$ ֜2 +13 "carousel0"F% +3 "core" Ƈ%4Pk \ +2 ."detached from brent"Carousel.configure :default +4 "carousel0"F#<` F 22 +10 ."detached from brent"Carousel.hold +6 "carousel0"F+ +9 "core" ƈ+ +3 ."detached from brent"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 "ambient0"J % +3 "core" ʉ%e +3 ."detached from brent"Abs.configure :default +2 "ambient0"J#  +9 "carousel1"G\ & +3 "core" NJ&LQ +3 ."detached from brent"PhaseSeparator.configure :default + "carousel1"G$ +9 "syringe0"B & +3 "core" ‹&*, +3 ."detached from brent"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from brent"Archive.hold + "syringe0"B , +5 "core" Œ, +3 ."detached from brent"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 "syringe1"C % +3 "core" Í%68 +3 ."detached from brent"Air.configure :default + "syringe1"C#d P]z<2 +8 ."detached from brent"Air.hold +2 "syringe1"C+ +5 "core" Î+ +3 ."detached from brent"Air.configure :default +2 "syringe1"C#d P]z<2 +7 C& +3 "core" Ï&XJ +2 ."detached from brent"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from brent"Lysis1.hold +2 "syringe1"C, +5 "core" Ð, +3 ."detached from brent"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B% +3 "core" ‘%Y5A( +3 ."detached from brent"Lysis2.configure :default +4 "syringe0"B#d P\\k<2 +9 ."detached from brent"Lysis2.hold +2 "syringe0"B+ +6 "core" ’+ +2 ."detached from brent"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +10 "valve1"E& +3 "core" œ& N;SO& K +3 ."detached from brent"PinPort.configure :default +2 "valve1"E$ B22 +7 ."detached from brent"PinPort.hold +2 "valve1"E, +6 "core" Ŕ, +3 ."detached from brent"PinPort.configure :default + "valve1"E$ B22 +7 #"detached from brent"All elf controllers running firmware version 4.94 +7 "valve0"D% +4 "core" ĕ%ZG-O- / +5 "valve0"D& +3 "carousel0"F% +7 "syringe0"B& +7 "syringe1"C% +6 C& +10 "valve1"E& +4 "syringe0"B% +7 "core" Ė&O(  + Ɨ%4NQ  + ˜&)7 + Ù%6 + Ú&X= + Ŝ& N;CO" [ + ›%Y5; +9 ."clearToroid"Toroid.seek :clear +7 "valve1"E& +7 "valve0"D( +13 "core" ŝ& N;UO' I +4 Ğ( +3 ."detached from brent"Air.seek :clear +3 "syringe1"C' +124 #"brent"-> exit +2 #Session Ended +118 "core" ß' +3 ."detached from brent"Lysis1.seek :clear +2 "syringe1"C ( +629 #"brent"Session Begun +750 "core" à( +3 ."detached from brent"Lysis2.seek :clear +4 "syringe0"B!'j +42 #"brent"-> showlog +1332 "core" ¡' +3 ."detached from brent"Archive.seek :clear +3 "syringe0"B"(j +1855 "core" ¢( +2 ."detached from brent"Carousel.in :default +2 "carousel0"F><` F 22 +7 F#% +3 "core" ƣ%4RIP  +41 ."detached from brent"Carousel.home.seek 55 +3 "carousel0"F$'4 +26 "core" Ƥ' +2 "valve0"D%% +4 "core" ĥ%ZG)O 3 +2 "syringe1"C&& +4 "core" æ&TbQ +5 #"detached from brent"Selecting Cartridge 42 +2 "valve0"D'% +8 "core" ħ%ZG7O % +8 ."detached from brent"Gate.power :heat,:OFF +4 .Gate.power :cartridge,:OFF +5 "valve1"E(& +12 "core" Ũ& N;LO R +4 ."detached from brent"PinPort.in :unlocking +2 "valve1"E? B2 +10 ."detached from brent"PinPort.seek :clear +3 "valve1"E)( +81 "core" ũ( +2 ."detached from brent"Carousel.in :default +2 "carousel0"F><` F 22 +7 F*% +3 "core" ƪ%4XFQ#  +4 ."detached from brent"Carousel.home.seek 55 +2 "carousel0"F+'4 +18 "core" ƫ' +2 "carousel0"F,% +4 "core" Ƭ%4WQR  +4 "syringe0"B-& +4 "core" ­&j'Z +3 "syringe1"C.% +3 "core" î%݋X( +3 "syringe0"B/% +4 "core" ¯%jٟR +3 "syringe1"C0& +5 "core" ð&YV +2 "valve0"D1% +5 "core" ı%ZG'O 5 +3 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"D2'8 +97 "core" IJ' +3 ."detached from brent"Carousel.seek 42 +7 "carousel0"F3'( +289 "core" Ƴ' +4 "carousel0"F4% +3 "core" ƴ%(2@T 2 +3 ."detached from brent"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +9 "syringe1"C5& +5 "core" õ&RY +7 ."detached from brent"Lysis1.in :fast +2 "syringe1"C?d P[<2 +13 ."detached from brent"Lysis1.seek :locked +6 "syringe1"C6( +248 "core" ö( +3 "valve0"D7% +6 "core" ķ%8ZO +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 "syringe1"C8% +4 ."Plunge"Plunge.seek :locked +9 "valve0"D9'Z +3 "core" ø%ݍP +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +9 ."Air"Air.seek :locked +4 "syringe1"C:'6 +73 "core" Ĺ' +33 ú' +2 ."detached from brent"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F;% +3 "core" ƻ%(Y/^P  +7 #"detached from brent"Powering cartridge bus +264 "cartridge_broadcast" ( +103 ."detached from brent"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +8 ."detached from brent"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from brent"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y  +5 "cartridge" y /J59993 /CF +3 #"detached from brent"Mounted lysis Cartridge board#J59993 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"D?% +4 "core" Ŀ%ZG!O2 ; +4 "syringe1"C@& +3 "core" & I +3 "syringe1"CA% +4 "core" %6 > +3 "carousel0"FB% +3 "core" %(0Pn \  +4 "carousel0"FC% +5 "core" %(/P{ \  +118 ."PROCESSING"SPR.command "read_sequences" +80 .SPR.command "stats_on" +8 "carousel0"FD% +4 "core" %(/Pp \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3102h.spr.bz2 +197 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CE% +6 "core" %6  G +44 "syringe1"CF% +3 "core" %6\  H +47 "syringe1"CG% +4 "core" %6Ȃ @ +46 "syringe1"CH% +4 "core" %6  G +46 "syringe1"CI% +4 "core" %6 D +46 "syringe1"CJ% +3 "core" %6 O +47 "syringe1"CK% +3 "core" %6  E +47 "syringe1"CL% +4 "core" %6 : +47 "syringe1"CM% +4 "core" %6 K +46 "syringe1"CN% +4 "core" %6 ~ G +46 "syringe1"CO% +3 "core" %6~ C +47 "syringe1"CP% +3 "core" %6 ~ F +47 "syringe1"CQ% +3 "core" %6 D +47 "syringe1"CR% +6 "core" %6 { I +51 "Can"m]+zsu5 +2 #"PROCESSING"Syringe tare is 51% of full scale at 22C (drifted -0%) +3 .PinPort.in :locking +4 "valve1"E? B22 +19 ."PROCESSING"PinPort.seek :locked +3 "valve1"ES( N +79 "core" ( +3 ."posArchive"Archive.in :fast +6 ."posLyS2"Lysis2.in :fast +8 "syringe0"B?d PZ<2 +7 ."PROCESSING"Lysis1.seek 1800uL +4 "syringe0"B>d P\\k<2 +6 ."posArchive"Archive.seek :empty +11 "syringe1"CT( +5 "syringe0"BU( +7 ."posLyS2"Lysis2.seek 1800uL +12 "syringe0"BV' +99 "Can"h]\\+zrr~ !` +19 "core" ( +87 ' +613 ( +5 ."PROCESSING"delayUntil "02:39:35.01" +2972 #"brent"-> exit +2 #Session Ended +8273 "Can"k]+zxuQ~i#` +11971 k\\+zquO{dA` +11972 l\\+zsuL]_` +11974 l]5+zsuP0U}` +11028 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +4 BW% +3 "core" % S +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +4 CX& +3 "core" &S j +47 ."Lysis2mixer"Lysis2.seek 1550uL +6 ."PROCESSING"Lysis1.seek 1550uL +4 "syringe0"BY' + "syringe1"CZ( +868 "Can"i^+zqs] ` +2616 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +46 ."PROCESSING"SPR.command "start 2 14" +50 #SPR stabilized +4 "syringe1"C[& +6 "core" &Ix  +8 "syringe0"B\\% +3 "core" %.x +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +256 #Waiting up to 11:00 for SPR to enter state 1 +6468 "Can"l^+zquB` +5300 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B]% +4 "core" %7 +4 "syringe1"C^& +4 "core" &M  +46 ."Lysis2mixer"Lysis2.seek 1250uL +6 "syringe0"B_' +6 ."PROCESSING"Lysis1.seek 1250uL +6 "syringe1"C`( +4173 "core" ' +5 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +360 #Waiting up to 1:00:00 for SPR to enter state 7 +414 "Can"i^+zqt--` +11972 l^+zku@H` +3433 #"espclient"Session Begun +836 #-> showlog 10 +270 #-> exit +2 #Session Ended +7429 "Can"k]+zsu?` +11969 k] +zqu; 1` +11972 l\\+zmu?$O` +11974 l\\r+zqu@']m` +11975 l\\[+zru?*` +11971 l\\+zru>.` +11971 l]E+zsu@1R` +1414 #"SPRlogger"SPRsummary:3.08e+09RIU,1e+09RIU,1.49RIU,1e+09RIU after 13635s +10558 "Can"k^!+ztu!5?` +11970 l^+zpu"9;` +11969 l^+zru6C=.!` +11973 m^+zru"@?` +11978 k]+zqu"*D]` +11972 l]t+zru=RH{` +11972 k]-+zru@Kg` +3302 "syringe1"Ca& +3 "core" &XI V +4 "syringe0"Bb% +3 "core" %`L +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +362 #Waiting up to 11:00 for SPR to enter state 1 +6294 "Can"n]+zruBN` +5516 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Bc% +4 "core" % +5 "syringe1"Cd& +4 "core" &" J +47 ."Lysis2mixer"Lysis2.seek 1100uL +5 ."PROCESSING"Lysis1.seek 1100uL +3 "syringe0"Be'" +9 "syringe1"Cf(" +2088 "core" ' +7 ( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +252 #Waiting up to 1:00:00 for SPR to enter state 7 +2336 "Can"k\\+zqt-1T{` +11973 l\\x+ztu;X<` +11970 l\\+zsu=[` +11971 l]+zru:^/` +11973 m^+zsu<bM` +11973 l^+zut)1e`k` +11973 m^m+zqu@Oh` +11972 l^+zquBl ` +11973 n]+zsu"Lo\\` +11973 k]b+zqu"s]` +11973 j\\+zru"wZ ` +11974 m\\+zsu:J{2 ` +11974 l\\+zqu "~ =` +11972 m]+z~u"? [` +11970 k^+z{u?W# y` +11969 i_(+zu@o ` +1242 "syringe1"Cg& +4 "core" &"h  +5 "syringe0"Bh% +4 "core" %""j! +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +360 #Waiting up to 11:00 for SPR to enter state 1 +8353 "Can"l^+zu> ` +3458 ."PROCESSING"SPR.command "hold_on" +188 #SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 +2 "syringe0"Bi% +8 "core" %"R +4 "syringe1"Cj& +5 "core" &"~Q/ +42 ."Lysis2mixer"Lysis2.seek 950uL +5 "syringe0"Bk' +2 ."PROCESSING"Lysis1.seek 950uL +7 "syringe1"Cl( +2089 "core" ' +6 ( +4 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +252 #Waiting up to 1:00:00 for SPR to enter state 7 +4396 "Can"l^t+zxt-I ` +11974 k]+zpu@'G ` +11972 k]F+zru9!` +11974 i\\+zquA!-` +11974 m\\+ztuA!K` +11975 i\\+ztuB!i` +11974 j\\+zsu;!` +11976 m]+zqu@!` +11973 k^q+zvu"4!` +11972 l^Q+zqu "!` +11976 l]+ztu"!` +11976 l]+zsu@eB"` +11977 l\\+zru"*";` +11975 k\\R+zfu!"Y` +11974 m\\d+zqu=4"w` +11124 #"PROCESSING"SPR analysis completed +852 "Can"j\\M+zru@{"` +1535 #"SPRlogger"Ended log spr26jul3102h.spr.bz2 + ."PROCESSING"SPR.command "stats_off" +16 "carousel0"Fm% +3 "core" %(-~P \  +10423 "Can"m\\ +zpu@|ʵ"` +11976 k[+ztuA["` +11974 k\\+ztu@g$"` +11975 m]u+zsuBqZ# ` +11973 i]+zru=f׏#+` +11973 k]+ztuB`#I` +11975 l]n+zvu@Z#g` +11975 n\\+ztuAd.#` +11975 l\\+zruALb#` +11977 j\\3+ztu?J#` +11974 l[+zsu@a#` +11971 j[+ztuAE#` +11972 j\\8+zru<^/$` +11976 k]+zluCUa$9` +11979 l]+zguCS$W` +11976 j]1+z}u@G$u` +11975 o\\+zqu@4$` +11973 m\\+zsu@I*$` +11974 j[+zruB4\\$` +11973 j[+zsuC9$` +11976 l[+zsuBN\ % ` +11976 l\\+zquCG %)` +11971 j\\+zuD3!%G` +11973 j]+zsuCOS%e` +11971 l\\+ztuD7%` +11974 l\\r+zuuB2%` +11974 k\\ +zruDP%` +11970 j[+zwuB<!%` +11975 m[+zzu>H$J%` +11973 l\\f+zsuA/'{&` +11973 j]+zqu?7*&7` +11971 i]+zsu>I-&U` +11974 l]$+zsuA.1 &s` +11976 l\\]+zvuA04=&` +11974 m\\+zru<S7n&` +11972 j[+ztuDU:&` +11971 j[y+zsuB8=&` +11972 l\\@+zsuC>@' ` +11973 m\\+zquE/D/''` +11974 k]+z}uBJG`'E` +11972 l\\+zru@EJ'c` +11971 m\\,+zquE4M'` +11971 l[+zuuC8P'` +11973 j[+zruC5T '` +11974 k["+ztuC4WP'` +11975 k[+zuB=Z'` +11973 k\\ +zquA(](` +11972 m\\+zquA'`(5` +11972 m\\+ztuC(d(S` +11973 m\\+zsuF/g>(q` +11974 k\\N+zsuC:jn(` +11974 k[+zquF)m(` +11973 k[=+zpuE4p(` +11972 mZ+zouF/s(` +11970 lZ+zu@+w0)` +11973 lZ+zsuA3za)%` +11971 j[+zuu?+})C` +11972 m\\+zvu>v)a` +11975 l[+zquC6)` +11974 j[+zpu@1!)` +11974 m[%+zru@4P)` +11972 kZ+ztuBG)` +11970 kZ+zpuC>)` +11970 nZ+zfu?0*` +11974 nZ+ztu>c*3` +11971 m[+zsuD<A*Q` +11972 j[+ztuH r*o` +11975 k\\)+zsu@0*` +11969 k\\4+ztuD.*` +11968 k\\+zsuE<*` +11973 n[+zquA41*` +11973 k[+zruC*a+` +11975 l[+ztu=D+#` +11975 m[^+ztuAK+A` +11972 m[,+zru@I+_` +11974 lZ+zruA8"+}` +11973 kZ+zruC%R+` +11975 lZ+zru@C+` +11975 m[+zsuA2ó+` +11973 k[Q+ztu<+` +11973 l[+ztuB2,` +11973 m[+zsuE1B,1` +11971 n[+z~uA#r,O` +11971 k[+zru@BӢ,m` +11968 m[+zqu?C,` +11967 j[+zzu@B,` +11970 l[+zou?25,` +11968 m[+ziu=:e,` +11969 m[+zduB5-` +11970 j[+zuuA.-!` +11967 k[+zhuBI-?` +11969 k[+znuD#%-]` +11973 j[+zmu?3U-{` +11975 k[s+zquA--` +11971 j[G+zwu?=-` +11968 k[a+zsuC:-` +11969 k[+zquB;-` +11972 l[p,zqu@3H.` +11970 m[{,\ ziu@Dz./` +11967 k[a,zeu=q.M` +11968 kZ,z]u@@ .k` +11969 iZ,zZu@+ .` +11973 l[,,z]u?C<.` +11970 k[,zhuC(l.` +11971 k\\,zRu>A.` +11972 m\\,zWuA7/` +11970 j\\,zmu@F/` +11969 k\\k,zpuC4 ./=` +11967 l[,zfuD9#^/[` +11967 m[~,\ zquAL&/y` +11967 j[Y,zquA,)/` +11970 k[-+zou@?,/` +11967 mZ,zquAm0/` +11971 kZ,zfuD)3M/` +11970 l[8+zru@)6}0` +11971 n[t+zruB;90-` +11971 m[+zruA4<0K` +11969 l[+zquB:@0i` +11971 l\\+zquB6C@0` +11973 m\\,+zsu=aFq0` +11972 m\\6+zru@9I0` +11968 k\\0+zru;SL0` +11970 l\\!+ztu@?P0` +11971 l\\.+zqu?S51` +11970 l\\+zqu?AVf1;` +11965 k\\>+zquA5Y1Y` +11969 l\\:+zru?E\\1w` +11966 k\\+zouA?_1` +11971 k[+zju?Kc*1` +11971 k[+zsuBAf[1` +11973 k[,zquAi1` +11970 m[+zru?l2 ` +11970 k\\+zsuA"o2+` +11973 j],+zquE.s2I` +11976 i],zquC7v?2g` +11975 l],zquBVyq2` +11974 k],zkuA5|2` +11976 m]~,zgu>i2` +11971 l]/,zhu<U2` +11970 k\\,zluB282` +11971 m\\[,zau?Bj3` +11973 l\\,"zsu<N39` +11975 k[,!zru@E3W` +11974 l[,zfu;J3u` +11974 k[,#zru>x13` +11972 m[,(zsu?Dd3` +11969 k]),%zcu?O3` +11973 m]m,(ztu=q3` +11970 m],-zqu@K4 ` +11970 j]v,0zqu?]+4)` +11972 n\\,/zsu>b]4G` +11970 k\\,%zpu?L4e` +11966 l\\`, zgu@E4` +11970 l\\R,ztu@U4` +11969 m\\6, zru>2&4` +11972 k\\,zsu=KY4` +11972 m],zquA_4` +11971 n_ ,ziu>W5` +11967 k_.,zru@B57` +11970 l^,zsu?R$5U` +11971 k^N,zgu>TV5s` +11969 l]q,zfu>_̉5` +11969 k],%zsuA@Ͻ5` +9681 #"preston"Session Begun +2290 "Can"l\\,*zsu>X5` +6357 #"preston"-> Cart.unlock +6 .Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +4 "valve1"En& +6 "core" & N;iO?  +4 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."preston"PinPort.seek :clear +3 "valve1"Eo( +81 "core" ( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +10 Fp% +3 "core" %(.P> \  +3 ."preston"Carousel.home.seek 42 +5 "carousel0"Fq'( +16 "core" ' +2 "carousel0"Fr% +4 "core" %(%4S 2 +3 "syringe0"Bs& +4 "core" &)X0 +5 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +6 "syringe1"Ct% +2 ."Archive"Archive.seek :clear +6 "syringe0"Bu(j +5 "core" %6 b +4 ."Air"Air.in :unlocking +5 "syringe1"C> P]z-2 +4 "syringe0"Bv% +6 "core" %z  +2 ."Air"Air.seek :clear +8 "syringe1"Cw' +4 ."Lysis2"Lysis2.in :unlocking +5 "syringe0"B> P\\k(2 +8 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bx'j +79 "core" ' +194 ' +134 ( +2 "syringe1"Cy& +5 "core" &zނ, ~ +4 ."Lysis1"Lysis1.in :unlocking +3 "valve0"Dz% +4 "syringe1"C? P[(2 +6 "core" %ZG%MO/ 7 +3 ."Lysis1"Lysis1.seek :clear +5 ."Plunge"Plunge.in :unlocking +5 "valve0"D> P ޤ(2 +4 "syringe1"C{( +5 ."Plunge"Plunge.seek :clear +4 "valve0"D|'8 +97 "core" ' +170 ( +4642 #"preston"-> SC.to 50 +5 "Can"l\\,zju5ր5` +2 ."preston"Carousel.seek 50 +3 "carousel0"F}'/ +188 "core" ' +2 "carousel0"F~% +5 "core" %/*DS 2 +2367 #"preston"-> SC.to 20 +5 .Carousel.seek 20 +4 "carousel0"F' +633 "core" ' +2 "carousel0"F% +4 "core" Ɓ%6S_Tn 2 +2183 #"preston"-> slots 1..60=>nil +2317 #-> ESP.shutdown +6 "sampler0"H% +3 "core" Ȃ%H +4 ."closeIntake"Intake.close +6 ."closeExhaust"Exhaust.close +6 "sampler0"H/ +5 ."closeBypass"Bypass.close +5 "sampler0"H0 +3 ."closeHandoff"Handoff.select :close +11 "sampler1"I/ +4 "valve0"D +15 "core" Ȅ/ + ȅ0 ɇ/ +46 ."preston"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +9 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +171 #-> exit +2 #Session Ended @1785514267.40EDT = =mMAIN_scheduler =tMAIN @EDT1785514268.53 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +157 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +224 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +379 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +72 #Sea pressure (Abs) tare is 6% of full scale +348 "Can"md+zwu@H + md+zwu@H + #tPolling Can every 2:00 +53 "Can"ld+ztuAH +1058 #t-> ESP.ready! +4 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +2 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +21 #Powering core bus +221 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +46 "valve0"DP +3 D +2 "core" ā +7 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +6 .tSamplePump.configure :default +2 "sampler0"H#^ #(22 +7 .tToroid.configure :default +2 "valve0"D$ ֜2 +6 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +7 .tAbs.configure :default + "ambient0"J#  +7 .tPhaseSeparator.configure :default + "carousel1"G$ +7 .tArchive.configure :default + "syringe0"B$d PZ<2 +7 .tAir.configure :default + "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 .tPinPort.configure :default +2 "valve1"E$ B22 +4 #tAll elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +4 "valve0"D% +3 D& +4 "core" Ă%Z7N f +6 "carousel0"F% +8 "syringe0"B& +9 "syringe1"C% +4 ."Plunge_home"Plunge.home.jog 0 +19 "syringe1"C& +4 "syringe0"B% +5 "valve1"E & +4 "valve0"D\ ) +8 "core" ă&O$ +2 Ƅ%MalQ + +2 …&@e  Æ%ݒ  + Ç&rh B + ˆ%ٟ%  + ʼn&O# , Ċ) +10 ."PinPort_home"PinPort.home.jog 0 +8 ."Lysis2_home"Lysis2.home.jog 0 +2 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +3 "valve0"D % +4 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +2 ."Toroid_home"Toroid.home.jog 0 +3 "valve1"E * +3 "syringe0"B ) +6 "syringe1"C) +4 "carousel0"F) +6 "syringe1"C* +4 "syringe0"B* +3 "valve0"D* +11 "core" ċ%CZO(  + Ō* ) + Î) + Ð* + Ə) + ‘* + Ē* +2 "syringe0"B% +10 "syringe1"C& +4 "valve1"E& +4 "syringe1"C% +2 "syringe0"B& +2 ."Plunge_home"Plunge.in :homing +14 "valve0"D& +4 D> (2 +4 "core" “%sٞd  +2 Ô&r) + Ŗ&O + ×%ݔc + •&{C# Ę&O +10 ."Toroid_home"Toroid.in :homing +11 ."Plunge_home"Plunge.jog 590 +45 ."Lysis1_home"Lysis1.in :homing +7 ."Air_home"Air.in :homing +2 ."Lysis2_home"Lysis2.in :homing +3 ."Archive_home"Archive.in :homing +2 ."PinPort_home"PinPort.in :homing +4 "valve0"D?2 + D)N +2 "syringe1"C? (2 +7 C> -2 +3 "syringe0"B> (2 +2 B? -2 +2 "valve1"E?2 +7 ."Toroid_home"Toroid.jog 1365 +11 ."Lysis1_home"Lysis1.jog 1597 +2 ."Air_home"Air.jog 1430 +4 ."PinPort_home"PinPort.jog 1235 +4 ."Archive_home"Archive.jog 1611 +6 ."Lysis2_home"Lysis2.jog 1620 +3 "valve0"D*U + "syringe1"C*= +2 C) +3 "valve1"E* +8 "syringe0"B*K +4 B)T +17 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +2 "valve0"D ' +23 "core" Ġ' +5 ."Plunge_home"Plunge.in :default +3 "valve0"D> P ޤ-2 +46 "core" Ü) +2 ."Air_home"Air.setRawPosition! 0 +3 "syringe1"C7 +4 ."Air_home"Air.seek :home +3 "syringe1"C!' +3 "core" Û* +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 "syringe1"C82 +4 "core" á' + ž* + Ÿ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +6 ."Air_home"Air.in :default +2 ."Archive_home"Archive.setRawPosition! 50 +4 ."Lysis1_home"Lysis1.seek :home +2 "syringe0"B72 +2 "syringe1"C>d P]z<2 +5 "syringe0"B82 +5 "syringe1"C"( +5 ."Lysis2_home"Lysis2.seek :home +6 ."Archive_home"Archive.seek :home +4 "syringe0"B#' +3 B$( +9 "core" ŝ* + â( +3 ."Lysis1_home"Lysis1.in :default +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "syringe1"C?d P[<2 +3 "valve1"E8 N +8 ."PinPort_home"PinPort.seek :home +3 "valve1"E%( +2 "core" £' + ¤( +5 ."Lysis2_home"Lysis2.in :default +4 ."Archive_home"Archive.in :default +2 "syringe0"B>d P\\k<2 +2 B?d PZ<2 +207 "core" Ě* + ."Toroid_home"Toroid.setRawPosition! 0 +3 "valve0"D8 +4 ."Toroid_home"Toroid.seek :home +2 "valve0"D&( +3 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +4 "valve0"D? ֜2 +189 "core" ť( +2 ."PinPort_home"PinPort.in :default +3 "valve1"E? B22 +33 ."clearToroid"Toroid.seek :clear +4 "valve1"E'& +3 "valve0"D(( +4 "core" ŧ&’O n +5 .tPinPort.in :unlocking +2 "valve1"E? B2 +4 .tPinPort.seek :clear +3 "valve1"E)( +40 "core" ũ( +299 Ĩ( +3 .tAir.seek :clear +2 "syringe1"C*' +8 "core" ê' +3 .tLysis1.seek :clear +3 "syringe1"C+( +67 "core" ë( +2 .tLysis2.seek :clear +3 "syringe0"B,'j +74 "core" ¬' +2 .tArchive.seek :clear +3 "syringe0"B-(j +73 "core" ­( +3 .tCarousel.in :default +2 "carousel0"F><` F 22 +3 F.% +4 "core" Ʈ%Vak=Q  +3 .tCarousel.home.seek 23 +3 "carousel0"F/' +29 "core" Ư' +2 "valve0"D0% +4 "core" İ%^\ O@ ! +3 "valve0"D1% +4 "core" ı%^O@ ! +4 "sampler0"H2% +3 "core" Ȳ%R +4 "valve0"D3& +6 "core" ij&RO: ` +4 "carousel0"F4% +4 "core" ƴ%V_hQ2  +3 "ambient0"J5% +4 "core" ʵ%zU +3 "carousel1"G6& +5 "core" Ƕ&WQ +2 "syringe0"B7& +3 "core" ·&j2Z +3 "syringe1"C8% +5 "core" ø%ݤR +2 "syringe1"C9& +5 "core" ù&cP +2 "syringe0"B:% +5 "core" º%j٠T +3 "valve1"E;& +4 "core" Ż&%O2  +3225 #t-> slots +2076 #-> to 20 +2 "valve0"D<% +3 "core" ļ%^O !| +3 "syringe1"C=& +3 "core" ý&^j +3 "syringe1"C>% +4 "core" þ%ݝj +2 "valve1"E?& +5 "core" ſ&"O  +3 .tCarousel.seek 20 +2 "carousel0"F@' +83 "core" ' +4 "carousel0"FA% +3 "core" %6S_T4 2 +3 .tCarousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"CB& +5 "core" &ah +4 .tLysis1.in :fast + "syringe1"C?d P[<2 +4 .tLysis1.seek :locked +3 "syringe1"CC( +247 "core" ( +2 "valve0"DD% +6 "core" %^Oq !v +4 ."Plunge"Plunge.seek :locked +7 "valve0"DE'Z +6 "syringe1"CF% +6 "core" %ݝh +8 ."Air"Air.in :fast + "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"CG'6 +77 "core" ' +25 ' +3 .tCarousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +5 FH% +3 "core" %ZS_rQ  +7 #tPowering cartridge bus +279 "cartridge_broadcast" ( +16 .tGate.power :cartridge,:OFF +3 #Mounted archive Cartridge +781 #-> cart.unlock +3 !o:NoMethodError : mesgu:NameError::message4private method `unlock' called for :cart:Symbol: name: unlock: args[:bt["(ESP):4:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +813 #-> Cart.unlock +4 "valve1"EI& +3 "core" &#BO-  +2 .tCarousel.in :default +2 "carousel0"F><` F 22 +4 FJ% +3 "core" %S_0PU \ +3 .tCarousel.home.seek 20 +3 "carousel0"FK' +14 "core" ' +3 "carousel0"FL% +5 "core" %#S`XS 2 +2 "syringe0"BM& +5 "core" &jd +2 "syringe1"CN% +3 "core" %6 Y +5 ."Air"Air.in :unlocking +4 "syringe0"BO% + "syringe1"C> P]z-2 +11 ."Air"Air.seek :clear +3 "syringe1"CP' +4 "core" %jٞY +89 ' +2 "syringe1"CQ& +5 "core" & +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"DR% +2 "syringe1"C? P[(2 +6 "core" %ZG#O 9 +3 ."Lysis1"Lysis1.seek :clear +7 ."Plunge"Plunge.in :unlocking +3 "syringe1"CS( +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DT'8 +94 "core" ' +24 ( +618 "Can"lc+z]uODf +11964 mbU,zeuP! +3759 #t-> SC.to 40 +3 .Carousel.seek 40 +3 "carousel0"FU'& +429 "core" ' +2 "carousel0"FV% +4 "core" %&5T 2 +7765 "Can"la,zTuO\ +6228 #t-> slots +4783 #-> slot 1..60=>nil +388 #-> slots +563 "Can"ka,,zeuP +11964 j`,6zauOޫ +6464 #t-> SPR.start +114 .SPR.command "read_sequences" +66 .SPR.command "stats_on" +87 ."SPRlogger"SPR.start /var/log/esp/spr26jul3112h.spr.bz2 +5235 "Can"k`,Bz^uP +122 #t-> SPR.command +3 !o:ArgumentError: mesg"(wrong number of arguments (0 for 1):bt[ "(ESP):11:in `command'"(ESP):11:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +8007 #-> SPR.command "start 2 9" + .SPR.command "start 2 9" +3834 "Can"l`5,Lzku!!" +9399 #t-> SPR.command "start 2 14" +2 .SPR.command "start 2 14" +2565 "Can"j`8,PzcuM}8 +7 #t-> SPR.command "start 2 7" +2 .SPR.command "start 2 7" +3757 #-> SPR.command "start 2 14" +2 .SPR.command "start 2 14" +1697 #-> SPR.command "start 2 9" +3 .SPR.command "start 2 9" +6496 "Can"j`,Vzau" 3V +5582 #t-> SPR.command "start 2 14" +2 .SPR.command "start 2 14" +1507 #-> SPR.stop +249 #"SPRlogger"Ended log spr26jul3112h.spr.bz2 +3 .tSPR.command "stats_off" +4619 "Can"m_,]zouP!t +11964 l_,]z\\uO$ +6431 #t-> slot[40, 20]=>[:microSPR_phyto] +2 !o:SyntaxError: mesg"|compile error\ (ESP):19: syntax error, unexpected tASSOC, expecting $end\ slot[40, 20]=>[:microSPR_phyto]\ ^:bt["(ESP):19:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'":0 +547 #-> slots +4984 "Can"l_,azYuK' +516 #t-> slot [40, 20]=>[:microSPR_phyto] +859 #-> slots +2164 #-> Cart.aspect +8427 "Can"l`",fzquM*Ϋ +11966 m`,lzsuP- +6432 #t-> Phytosampler.goalVolume +2 !o:NameError: mesg"(uninitialized constant Phytosampler: name:Phytosampler:bt["(ESP):24:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'":0 +2140 #-> PhytoSampler.goalVolume +3391 "Can"l_,rzpuO0\ +5983 #t-> SC.to 45 +3 .Carousel.seek 45 +2 "carousel0"FW'+ +124 "core" ' +3 "carousel0"FX% +4 "core" %+)T 2 +5843 "Can"j_,uzUuOE3( +897 #t-> SC.to 50 +3 .Carousel.seek 50 +3 "carousel0"FY'/ +123 "core" ' +2 "carousel0"FZ% +5 "core" %/4;{T0 2 +10935 "Can"l_,yzRuO6F +5189 #t-> slot [50, 45]=>[:archiveHiBiomass_bac] +593 #-> slots +1950 #-> exit +3 "sampler0"H[% +4 "core" %b7 +6 #mEXIT @1785516392.44EDT @1785517189.29EDT = =mMAIN_scheduler =tMAIN @EDT1785517190.33 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +146 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +25 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +61 "Can"m_,zXuJ#L" +291 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +74 #Sea pressure (Abs) tare is 6% of full scale +74 !o:NoMethodError : mesg"7undefined method `lyseHold_phyto' for Object:Class: name0: args0:bt[";/home/esp/esp2/lib/framework.rb:51:in `method_missing'";/home/esp/esp2/lib/framework.rb:63:in `method_missing'"//home/esp/esp2/protocol/3G/cartTypes.rb:21"=/home/esp/esp2/protocol/3G/common3Gcfg.rb:8:in `require'"0/home/esp/esp2/protocol/3G/common3Gcfg.rb:8"B/home/esp/esp2/protocol/3G/function/config.rb:60:in `require'"5/home/esp/esp2/protocol/3G/function/config.rb:60"4/home/esp/esp2/utils/init3g.rb:256:in `require'"'/home/esp/esp2/utils/init3g.rb:256"9/home/esp/esp2/type/auv/initialize.rb:9:in `require'",/home/esp/esp2/type/auv/initialize.rb:9"2/home/esp/esp2/lib/framework.rb:124:in `load'"2/home/esp/esp2/lib/framework.rb:124:in `load'"5/home/esp/esp2/lib/framework.rb:182:in `execute'"-/home/esp/esp2/lib/esp.rb:120:in `shell'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +1566 #-> exit +5 #mEXIT @1785517212.83EDT @1785517310.25EDT = =mMAIN_scheduler =tMAIN @EDT1785517311.31 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +160 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +27 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +69 "Can"n_,zkuK.OA +310 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +76 #Sea pressure (Abs) tare is 6% of full scale +360 "Can"n_,zku5PC + n_,zku5PC + #tPolling Can every 2:00 +53 "Can"m_,zcuOPC/ +208 #t-> hold +850 #-> :hold +1664 #-> exit +5 #mEXIT @1785517349.22EDT @1785517752.94EDT = =tMAIN =mMAIN_scheduler @EDT1785517754.03 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +30 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +67 "Can"o_|,zWuF#Z +305 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +76 #Sea pressure (Abs) tare is 6% of full scale +356 "Can"o_|,zWu5"[. + o_|,zWu5"[. + #tPolling Can every 2:00 +52 "Can"l_P,zkuK[2, +1033 #t-> PhytoSampler.goalVolume +660 #-> BACsampler.goalVolume +486 #-> exit +5 #mEXIT @1785517786.34EDT @1785519225.68EDT = =mMAIN_scheduler =tMAIN @EDT1785519226.70 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +147 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +25 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +61 "Can"p^,zUuJLY!N +287 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +69 #Sea pressure (Abs) tare is 6% of full scale +339 "Can"p^,zUu60#& + p^,zUu60#& + #tPolling Can every 2:00 +55 "Can"m^,zRuF&#J +11960 l^,zQuMAJ +9519 #t-> slot 55 =>[:lyseHold_phyto] +2442 "Can"k_T,zQuJ_J +11959 l_,zSuN}J +3614 #t-> exit +4 #mEXIT @1785519631.62EDT @1785519660.68EDT = =tMAIN =mMAIN_scheduler @EDT1785519661.71 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +27 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +68 "Can"j_,zYuN`hk +292 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +76 #Sea pressure (Abs) tare is 6% of full scale +355 "Can"j_,zYu2?Z + j_,zYu2?Z + #tPolling Can every 2:00 +52 "Can"k_,zTuM} +2031 #t-> PumpPrimeDelay +3 !o:NameError: mesg"*uninitialized constant PumpPrimeDelay: name:PumpPrimeDelay:bt["(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +1477 #-> :PumpPrimeDelay +781 #-> Sampler.PumpPrimeDelay +3 !o:NoMethodError : mesg"Cundefined method `PumpPrimeDelay' for Cartridge::Sampler:Class: name0: args0:bt[ ";/home/esp/esp2/lib/framework.rb:51:in `method_missing'"(ESP):3:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +969 #-> exit +5 #mEXIT @1785519724.72EDT @1785519761.43EDT = =tMAIN =mMAIN_scheduler @EDT1785519762.48 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +154 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +27 #>BREAK< ESP3 Gateway v1.44 for core bus +4 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +62 "Can"l_1,zluHj +303 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +349 "Can"l_1,zlu49- +2 l_1,zlu49- #tPolling Can every 2:00 +53 "Can"k_(,z^uJ1 +439 #t-> Sampler.refill +2 .Bypass.open +2 "sampler1"I1 +155 "core" Ɂ1 +2 .tIntake.open +2 "sampler0"H1 +144 "core" Ȃ1 +2 .tSamplePump.setPosition! 0ml +18 .Gate.power :analytic1,:ON,limit to 4000mA + .Gate.power :analytic2,:ON,limit to 4000mA +2 .Gate.power :analytic3,:ON,limit to 4000mA +22 "valve0"DP +4 D +2 "core" ă +6 "valve0"D% +3 "core" Ą%8ZOE +3 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +4 .tPlunge.hold +2 "valve0"D+ +4 "core" ą+ +3 .tPlunge.configure :default + "valve0"D# P ޤ-2 +7 "sampler0"H% +3 "core" Ȇ%l +3 .tSamplePump.configure :default +2 "sampler0"H#^ #(22 +7 "valve0"D& +3 "core" ć&YOC Y +3 .tToroid.configure :default +2 "valve0"D$ ֜2 +5 .tToroid.hold + "valve0"D, +4 "core" Ĉ, +3 .tToroid.configure :default + "valve0"D$ ֜2 +7 "carousel0"F % +3 "core" Ɖ%/Y9Q]  +4 .tCarousel.configure :default + "carousel0"F#<` F 22 +4 .tCarousel.hold +2 "carousel0"F\ + +3 "core" Ɗ+ +3 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +11 "ambient0"J % +3 "core" ʋ%N +3 .tAbs.configure :default + "ambient0"J#  +8 "carousel1"G & +3 "core" nj&YQ^ +3 .tPhaseSeparator.configure :default + "carousel1"G$ +7 "syringe0"B & +3 "core" &j? +4 .tArchive.configure :default +2 "syringe0"B$d PZ<2 +4 .tArchive.hold +2 "syringe0"B, +3 "core" Ž, +3 .tArchive.configure :default + "syringe0"B$d PZ<2 +7 "syringe1"C% +3 "core" Ï%ݛK +4 .tAir.configure :default + "syringe1"C#d P]z<2 +5 .tAir.hold +33 "syringe1"C+ +5 "core" Ð+ +2 .tAir.configure :default +2 "syringe1"C#d P]z<2 +4 C& +3 "core" Ñ&YO +3 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 .tLysis1.hold +2 "syringe1"C, +3 "core" Ò, +3 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +4 "syringe0"B% +55 "core" “%jٛ +4 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +4 .tLysis2.hold + "syringe0"B+ +4 "core" ”+ +3 .tLysis2.configure :default + "syringe0"B#d P\\k<2 +7 "valve1"E& +3 "core" ŕ&#O5  +4 .tPinPort.configure :default + "valve1"E$ B22 +4 .tPinPort.hold +2 "valve1"E, +3 "core" Ŗ, +3 .tPinPort.configure :default + "valve1"E$ B22 +5 #tAll elf controllers running firmware version 4.94 +19 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +3 "sampler0"H7 +3 H% +3 "core" ȗ%n +4 .tSamplePump.seek 30ml +2 "sampler0"H'` +2700 #"p"Session Begun +359 #-> slots +930 "core" Ș' +2 .tBypass.close +2 "sampler1"I/ +152 "core" ə/ +2 .tExhaust.open +3 "sampler0"H2 +12 #"p"-> Cart.reserve 40,20,50,45 +135 "core" Ț2 +3 "sampler0"H% +4 "core" ț%`;~ +7 .tSamplePump.seek 55ml +3 "sampler0"H'-[ +296 #"p"-> Cart.dry +382 #-> Cart.reserved +2658 "core" Ȝ' +2 .tdelay 1 second +103 ."closeIntake"Intake.close +7 ."closeExhaust"Exhaust.close +8 "sampler0"H/ +2 "carousel0"F% +8 "sampler0"H0 +3 "core" ƞ%/Z8kQO  +134 ȝ/ +16 ȟ0 +2 "sampler0"H % +5 "core" Ƞ%-[nK +2828 "Can"j_-,z_uI$ +1307 #"p"-> to 55 +5 "valve0"D!% +6 "core" ġ%8ZO; +3 "syringe1"C"& +3 "core" â&Or +3 "syringe1"C#% +5 "core" ã%ݩr +2 "valve1"E$& +9 "core" Ť&"O=  +4 ."p"Carousel.seek 55 +3 "carousel0"F%'4 +127 "core" ƥ' +4 "carousel0"F&% +3 "core" Ʀ%48T4 2 +3 ."p"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +7 "syringe1"C'& +3 "core" ç&So +4 ."p"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."p"Lysis1.seek :locked +3 "syringe1"C(( +249 "core" è( +3 "valve0"D)% +8 "core" ĩ%8ZO +3 ."Plunge"Plunge.seek :locked +4 "valve0"D*'Z +6 "syringe1"C+% +7 "core" ë%ݥef +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +5 "syringe1"C,'6 +66 "core" Ī' +39 ì' +2 ."p"Carousel.reconfigure :locked +3 "carousel0"F#` F 2\ 2 +6 F-% +3 "core" ƭ%4[)Q@  +10 #"p"Powering cartridge bus +265 "cartridge_broadcast" ( +2 ."p"Gate.setIR 1750,5000,0,6 +15 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +7 ."p"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."p"Gate.power :heat,:ON,limit to 5000mA +6 "Cartridge" .t +3 "cartridge" t +2 "Cartridge" /y +5 "cartridge" yִjִj +3 "Cartridge" 0y  +3 "cartridge" y r/J59943 r/6Cr +5 #"p"Mounted lysis Cartridge board#J59943 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +3198 #-> Cart.sampler.prepareLGcart +4 .Handoff.select :waste +5 "valve0"D +49 #"p"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +5 "syringe1"C1% +9 "core" ñ%6K ? +40 "syringe1"C2% +3 "core" ò%6K N +47 "syringe1"C3% +3 "core" ó%6 N~ E +47 "syringe1"C4% +3 "core" ô%6Iu N +49 "syringe1"C5% +5 "core" õ%6 H{ F +43 "syringe1"C6% +3 "core" ö%6w D +47 "syringe1"C7% +3 "core" ÷%6  I +47 "syringe1"C8% +3 "core" ø%6 D +49 "syringe1"C9% +4 "core" ù%6  F +45 "syringe1"C:% +4 "core" ú%6 8 +46 "syringe1"C;% +3 "core" û%6  E +47 "syringe1"C<% +3 "core" ü%6 D +48 "syringe1"C=% +5 "core" ý%6 ; +44 "syringe1"C>% +3 "core" þ%6 J +47 "syringe1"C?% +3 "core" ÿ%6\  H +47 "syringe1"C@% +3 "core" %6 D +49 "syringe1"CA% +4 "core" %6  G +44 "syringe1"CB% +3 "core" %6 B +47 "syringe1"CC% +3 "core" %6 @ +4 "Can"j_-,z_u2 +2 #"p"Syringe tare is 51% of full scale at 24C (drifted -0%) +6 .PinPort.in :locking +2 "valve1"E? B22 +7 ."p"PinPort.seek :locked +3 "valve1"ED( N +80 "core" ( +4 ."posArchive"Archive.in :fast +10 ."posLyS2"Lysis2.in :fast +6 ."p"Lysis1.seek 1250uL +5 "syringe0"B?d PZ<2 +5 B>d P\\k<2 +2 "syringe1"CE( +12 ."posArchive"Archive.seek :empty +4 ."posLyS2"Lysis2.seek 950uL +6 "syringe0"BF( +5 BG' +231 "core" ( +269 ' +329 ( +2 "syringe0"BH& +4 "core" &%) < +1100 #"p"-> Cart.unlock +10 .Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +6 "valve1"EI& +4 "core" & N;?OW +4 ."p"PinPort.in :unlocking +3 "valve1"E? B2 +6 ."p"PinPort.seek :clear +3 "valve1"EJ( +81 "core" ( +2 ."p"Carousel.in :default +3 "carousel0"F><` F 22 +43 FK% +4 "core" %4RP \ +3 ."p"Carousel.home.seek 55 +5 "carousel0"FL'4 +15 "core" ' +2 "carousel0"FM% +8 "core" %4WlQ^  +3 "syringe0"BN& +4 "core" &*r +11 ."Archive"Archive.in :unlocking +4 "syringe1"CO% +3 "syringe0"B? PZ-2 +7 "core" %6v f +4 ."Archive"Archive.seek :clear +7 "syringe0"BP% +3 ."Air"Air.in :unlocking +11 "syringe0"BQ(j +3 "syringe1"C> P]z-2 +6 "core" %{\ s v +3 ."Air"Air.seek :clear +8 ."Lysis2"Lysis2.in :unlocking +6 "syringe1"CR' +3 "syringe0"B> P\\k(2 +9 ."Lysis2"Lysis2.seek :clear +4 "syringe0"BS'j +79 "core" ' +194 ' +148 ( +2 "syringe1"CT& +5 "core" &’o N +5 ."Lysis1"Lysis1.in :unlocking +6 "valve0"DU% +2 "syringe1"C? P[(2 +8 "core" %ZG#O? 9 +5 ."Lysis1"Lysis1.seek :clear +5 ."Plunge"Plunge.in :unlocking +7 "syringe1"CV( +4 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DW'8 +97 "core" ' +107 ( +2435 "Can"k_,z]u4 +224 #"p"-> slots +3700 #-> PhytoSampler.prime 10,10 +4 "carousel0"FX% +6 "core" %4XQ?  +4 #"p"Priming sample loop w/10ml, bypass w/10ml +3 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +6 ."p"Bypass.open +3 "sampler1"IY1 +153 "core" 1 +74 "ambient0"JZ% +3 "core" %. +3 ."p"Intake.open +4 "sampler0"H[1 +144 "core" 1 +2 "ambient0"J\\% +5 "core" %# +3 #"p"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H]% +8 "core" %:m +43 "sampler0"H^% +4 "core" %: e  +6 ."p"Abs.hold +2 "ambient0"Je+ +6 "core" + +4 "sampler0"Hf% +4 "core" %Y +3 "sampler0"Hg% +4 "core" %dO +5 ."p"SamplePump.in :start +3 "sampler0"H>^ }n #(@D3 22Q +8 ."p"SamplePump.seek 10ml,1:10 +6 "sampler0"Hh'6 +52 ."pumpStarted"SamplePump.configure :prime +3 "sampler0"H#d }n #(@D3 22Q +1286 "core" ' +3 "sampler0"Hi% +4 "core" %6I 2 +3 ."p"Abs.stop +3 "ambient0"J5 +6 ."p"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."p"Exhaust.open +3 "sampler0"Hj2 +153 "core" 2 +73 ."closeBypass"Bypass.close +5 "sampler1"Ik/ +150 "core" / +73 "sampler0"Hl% +3 "core" %Da +3 "ambient0"Jm% +4 "core" %_ +2 ."p"Abs.in :default +4 "ambient0"J>:7 e  +7 ."p"Abs.hold +2 "ambient0"Jn+ +6 "core" + +3 "ambient0"Jo% +5 "core" %fv +3 "sampler0"Hp% +5 "core" %=t +2 "sampler0"Hq% +5 "core" %D^ +5 ."p"SamplePump.in :start +3 "sampler0"H>^ }n #(@_D3 22Q +7 ."p"SamplePump.seek 10ml,1:10 +8 "sampler0"Hr'6 +51 ."pumpStarted"SamplePump.configure :prime +3 "sampler0"H#d }n #(@_D3 22Q +1290 "core" ' +2 "sampler0"Hs% +4 "core" %6 2 +3 ."p"Abs.stop +3 "ambient0"J5 +4024 "Can"k_8,z^u; +2589 #"p"-> PhytoSampler.primeVolume +719 #-> PhytoSampler.bypassVolume +6768 #t-> start {fire :lyseHold_phyto} +21 "valve0"Dt% +9 Du& +3 "core" %8Z)O@ +2 "carousel0"Fv% +7 "syringe0"Bw& +5 "syringe1"Cx% +5 Cy& +6 "syringe0"Bz% +6 "valve1"E{& +21 "core" &L,OB f + %4YQ3  + &j: + %ݤ + &S %jٝF + &'O9  +9 ."clearToroid"Toroid.seek :clear +5 "valve1"E|& +6 "valve0"D}( +5 "core" &&O@  +2 ( +5 ."detached from MAIN"Air.in :default +3 "syringe1"C>d P]z<2 +7 ."detached from MAIN"Air.seek :clear +3 "syringe1"C~' +8 "core" ' +5 ."detached from MAIN"Lysis1.in :default +3 "syringe1"C?d P[<2 +6 ."detached from MAIN"Lysis1.seek :clear +4 "syringe1"C( +6 "core" ( +3 ."detached from MAIN"Lysis2.in :default +3 "syringe0"B>d P\\k<2 +7 ."detached from MAIN"Lysis2.seek :clear +3 "syringe0"B'j +7 "core" ' +4 ."detached from MAIN"Archive.in :default +3 "syringe0"B?d PZ<2 +5 ."detached from MAIN"Archive.seek :clear +4 "syringe0"B(j +5 "core" ‚( +3 ."detached from MAIN"Carousel.in :default +3 "carousel0"F><` F 22 +6 F% +3 "core" ƃ%4YtQb  +4 ."detached from MAIN"Carousel.home.seek 55 +4 "carousel0"F'4 +16 "core" Ƅ' +3 "valve0"D% +5 "core" Ć%8ZO< +4 #"detached from MAIN"Selecting Cartridge 55 +3 "valve0"D% +7 "core" ć%8ZO= +3 "syringe1"C & +4 "core" É&PN +3 "syringe1"C % +4 "core" Ë%ݠ{ +3 "valve1"E & +4 "core" Ō& O=  +3 ."detached from MAIN"Carousel.seek 55 +4 "carousel0"F '4 +17 "core" ƍ' +4 "carousel0"F% +5 "core" Ə%4YQK  +3 ."detached from MAIN"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +6 "syringe1"C& +7 "core" Ñ&Ix +4 ."detached from MAIN"Lysis1.in :fast +3 "syringe1"C?d P[<2 +7 ."detached from MAIN"Lysis1.seek :locked +4 "syringe1"C( +250 "core" Ó( +3 "valve0"D% +7 "core" ĕ%8ZO +3 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +5 "syringe1"C% +6 ."Plunge"Plunge.seek :locked +6 "valve0"D'Z +6 "core" ×%ݡ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +8 ."Air"Air.seek :locked +3 "syringe1"C'6 +75 "core" ę' +30 Ú' +2 ."detached from MAIN"Carousel.reconfigure :locked +3 "carousel0"F#` F 2\ 2 +6 F% +3 "core" ƛ%4[DQ9  +7 #"detached from MAIN"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from MAIN"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +7 ."detached from MAIN"PuckHeater.configure :default +3 "Cartridge" \ :D H  +8 ."detached from MAIN"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" y +3 "cartridge" yִjִj +4 "Cartridge" y  +3 "cartridge" y r/J59943 r/6Cr +4 #"detached from MAIN"Mounted lysis Cartridge board#J59943 +8 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"D% +3 "core" ĝ%ZG:O> " +3 "syringe1"C& +10 "core" ß&p +2 "syringe1"C % +4 "core" à%6r K +4 "carousel0"F!% +4 "core" ơ%4P \ +3 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 55 +5 "valve0"D"% +8 "core" Ģ%ZGOA ? +3 "syringe1"C#& +5 "core" ã&n +2 "syringe1"C$% +4 "core" ä%6r D +4 ."sealing"Air.in :default +4 "carousel0"F&% +5 "syringe1"C>d P]z<2 +6 "core" Ʀ%4P \ +4 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +3 ."sealing"Air.seek :sealed +6 ."FILTERING"SamplePump.setPosition! 0ml +5 "syringe1"C'' +2 "sampler0"H7 +8 ."FILTERING"Exhaust.close +3 "sampler0"H\ 0 +148 "core" Ȋ0 +2 ."FILTERING"Bypass.open +3 "sampler1"I)1 +106 "core" ç' +44 ɩ1 +73 "ambient0"J+% +4 "core" ʫ%t2 +4 "Can"k_8,z^u3t! +2 "ambient0"J% +4 "core" ʒ%zD +3 "ambient0"J-% +3 "core" ʭ%xG +4 ."FILTERING"Abs.in :default +3 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"J+ +6 "core" ʅ+ +3 "sampler0"H% +5 "core" Ȑ%Uy +3 "sampler0"H.% +3 "core" Ȯ%Q +6 ."FILTERING"SamplePump.in :start +3 "sampler0"H>^ }n #(@D3 22Q +9 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"H/'6 +54 ."pumpStarted"SamplePump.configure :prime +3 "sampler0"H#d }n #(@D3 22Q +137 "Can"k_K,z`t0! +1150 "core" ȯ' +2 "sampler0"H0% +5 "core" Ȱ%6 2 +2 ."FILTERING"Abs.stop +4 "ambient0"J5 +7 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 ."FILTERING"Exhaust.open +4 "sampler0"H12 +150 "core" ȱ2 +74 ."closeBypass"Bypass.close +5 "sampler1"I2/ +151 "core" ɲ/ +73 "sampler0"H3% +3 "core" ȳ%Oy +3 "ambient0"J4% +5 "core" ʴ%U +2 ."FILTERING"Abs.in :default +4 "ambient0"J>:- e  +9 ."FILTERING"Abs.hold +3 "ambient0"J5+ +9 "core" ʵ+ +2 "ambient0"J6% +4 "core" ʶ%m +3 "sampler0"H7% +5 "core" ȷ%Rk +2 "sampler0"H8% +4 "core" ȸ%4` +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n #(@_D3 22Q +9 ."FILTERING"SamplePump.seek 200ml,13:50 +4 "sampler0"H9'G +54 ."pumpStarted"SamplePump.configure :prime +3 "sampler0"H#d }n #(@_D3 22Q +1207 #"p"-> MainThread.exit +5 .tSamplePump.stop +2 "sampler0"H5 +4 "core" ȹ'3 j 2 +3 "sampler0"H:% +17 H;% +9 "core" Ⱥ%3i 2 + Ȼ%3}; +2 .tSamplePump.stop + "sampler0"H5 +9 ."closeIntake"Intake.close +7 ."closeExhaust"Exhaust.close +4 ."closeHandoff"Handoff.select :close +5 ."FILTERING"SamplePump.stop +10 "sampler0"H% +10 "core" ƾ%4P \ +110 ȼ/ +2 ."closeIntake"Intake.close +4 "sampler0"H?/ +8 "core" ȿ/ + Ƚ0 +3 ."closeExhaust"Exhaust.close +6 #mEXIT +2 "sampler0"H@0 +5 !"closeExhaust"o:ThreadError: mesg"deferring killed thread:bt['"3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"1/home/esp/esp2/lib/schedule.rb:544:in `stop'"1/home/esp/esp2/lib/schedule.rb:616:in `stop'":/home/esp/esp2/lib/i2c/gateway.rb:640:in `writeAcked'";/home/esp/esp2/lib/rmutex.rb:136:in `exclusive_unlock'":/home/esp/esp2/lib/i2c/gateway.rb:638:in `writeAcked'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'":/home/esp/esp2/lib/i2c/gateway.rb:633:in `writeAcked'"=/home/esp/esp2/lib/i2c/gateway.rb:629:in `writeAckedCmd'"8/home/esp/esp2/lib/i2c/gateway.rb:619:in `writeMsg'"5/home/esp/esp2/lib/i2c/gateway.rb:484:in `write'"5/home/esp/esp2/lib/i2c/gateway.rb:724:in `write'"1/home/esp/esp2/lib/i2c/sleepy.rb:502:in `<<'"1/home/esp/esp2/lib/i2c/msg.rb:779:in `write'"0/home/esp/esp2/lib/i2c/msg.rb:419:in `send'"2/home/esp/esp2/lib/i2c/servo.rb:971:in `send'"9/home/esp/esp2/lib/i2c/msg.rb:444:in `await1stReply'":/home/esp/esp2/lib/i2c/msg.rb:475:in `awaitOnlyReply'"4/home/esp/esp2/lib/i2c/servo.rb:1358:in `close'"1/home/esp/esp2/lib/openclose.rb:90:in `send'"8/home/esp/esp2/lib/openclose.rb:90:in `openOrClose'",/home/esp/esp2/lib/msg.rb:68:in `retry'"8/home/esp/esp2/lib/openclose.rb:89:in `openOrClose'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"8/home/esp/esp2/lib/openclose.rb:82:in `openOrClose'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"8/home/esp/esp2/lib/openclose.rb:81:in `openOrClose'"2/home/esp/esp2/lib/openclose.rb:53:in `close'"9/home/esp/esp2/utils/init3g.rb:146:in `closeValves!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 #until Exhaust finishes closing +6 #"p"Session Ended @1785520283.73EDT @1785520293.25EDT = =mMAIN_scheduler =tMAIN @EDT1785520295.12 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +146 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +2 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +34 "Can"o_O,zfuUY-@ +326 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +67 #Sea pressure (Abs) tare is 6% of full scale +356 "Can"o_O,zfuD/( + o_O,zfuD/( + #tPolling Can every 2:00 +4 #Waiting for espclients... +47 "Can"j_P,zduY/L +78 #"preston"Session Begun +443 #-> slot +2650 #-> start {fire :lyseHold_phyto} +36 ."detached from preston"Gate.power :analytic1,:ON,limit to 4000mA +2 .Gate.power :analytic2,:ON,limit to 4000mA +5 .Gate.power :analytic3,:ON,limit to 4000mA +56 "valve0"DP +5 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +5 "valve0"D +3 "core" ā +7 "valve0"D% +3 "core" Ă%ZGAO*  +2 ."detached from preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from preston"Plunge.hold +2 "valve0"D+ +6 "core" ă+ +3 ."detached from preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +8 "sampler0"H% +3 "core" Ȅ%3}v +3 ."detached from preston"SamplePump.configure :default + "sampler0"H#^ #(22 +10 "valve0"D& +3 "core" ą&aON Q +2 ."detached from preston"Toroid.configure :default +3 "valve0"D$ ֜2 +6 ."detached from preston"Toroid.hold +2 "valve0"D, +5 "core" Ć, +3 ."detached from preston"Toroid.configure :default +3 "valve0"D$ ֜2 +9 "carousel0"F% +3 "core" Ƈ%4PPx \ +2 ."detached from preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +7 ."detached from preston"Carousel.hold +2 "carousel0"F+ +5 "core" ƈ+ +3 ."detached from preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 "ambient0"J % +3 "core" ʉ%qI +3 ."detached from preston"Abs.configure :default + "ambient0"J#  +10 "carousel1"G\ & +3 "core" NJ&XQ^ +2 ."detached from preston"PhaseSeparator.configure :default +2 "carousel1"G$ +10 "syringe0"B & +3 "core" ‹&jW +5 ."detached from preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from preston"Archive.hold +2 "syringe0"B , +6 "core" Œ, +3 ."detached from preston"Archive.configure :default + "syringe0"B$d PZ<2 +9 "syringe1"C % +3 "core" Í% +3 ."detached from preston"Air.configure :default + "syringe1"C#d P]z<2 +8 ."detached from preston"Air.hold + "syringe1"C+ +6 "core" Î+ +2 ."detached from preston"Air.configure :default +3 "syringe1"C#d P]z<2 +6 C& +3 "core" Ï&{ +3 ."detached from preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from preston"Lysis1.hold +2 "syringe1"C, +5 "core" Ð, +3 ."detached from preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B% +3 "core" ‘%j٣v +3 ."detached from preston"Lysis2.configure :default + "syringe0"B#d P\\k<2 +8 ."detached from preston"Lysis2.hold +2 "syringe0"B+ +5 "core" ’+ +3 ."detached from preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 "valve1"E& +3 "core" œ&OM  +3 ."detached from preston"PinPort.configure :default +2 "valve1"E$ B22 +6 ."detached from preston"PinPort.hold +2 "valve1"E, +4 "core" Ŕ, +4 ."detached from preston"PinPort.configure :default + "valve1"E$ B22 +6 #"detached from preston"All elf controllers running firmware version 4.94 +7 "valve0"D% +3 D& +6 "core" ĕ%ZG)OM 3 +2 "carousel0"F% +7 "syringe0"B& +6 "syringe1"C% +5 C& +8 "syringe0"B% +14 "valve1"E& +15 "core" Ė&SOJ _ Ɨ%4XQe  + ˜&j$N + Ù% + Ú&Y ›%jْY + Ŝ&OK  +8 ."clearToroid"Toroid.seek :clear +4 "valve0"D( +2 "valve1"E& +12 "core" Ğ( + ŝ&O=  +3 ."detached from preston"Air.seek :clear +3 "syringe1"C' +470 "core" ß' +3 ."detached from preston"Lysis1.seek :clear +3 "syringe1"C ( +182 #"preston"-> exit +3 #Session Ended +217 "core" à( +3 ."detached from preston"Lysis2.seek :clear +2 "syringe0"B!'j +5 "core" ¡' +2 ."detached from preston"Archive.seek :clear +43 "syringe0"B"(j +12 "core" ¢( +3 ."detached from preston"Carousel.in :default +2 "carousel0"F><` F 22 +4 F#% +3 "core" ƣ%4XzQb  +3 ."detached from preston"Carousel.home.seek 55 +3 "carousel0"F$'4 +14 "core" Ƥ' +3 "valve0"D%% +4 "core" ĥ%ZG)O9 3 +2 "syringe1"C&& +5 "core" æ&Z +4 #"detached from preston"Selecting Cartridge 55 +2 "valve0"D'% +4 "core" ħ%ZG!O: ; +5 ."detached from preston"Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +2 "valve1"E(& +3 "core" Ũ& O:  +3 ."detached from preston"Carousel.in :default + "carousel0"F><` F 22 +4 F)% +3 "core" Ʃ%4YdQh  +5 ."detached from preston"Carousel.home.seek 55 +2 "carousel0"F*'4 +15 "core" ƪ' +2 "carousel0"F+% +4 "core" ƫ%4XQk  +3 "syringe0"B,& +4 "core" ¬&j/ +3 "syringe1"C-% +4 "core" í%ݜ +3 "syringe0"B.% +5 "core" ®%jٕ +2 "syringe1"C/& +5 "core" ï&O +2 "valve0"D0% +5 "core" İ%ZG,O> 0 +4 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +4 ."Plunge"Plunge.seek :clear +2 "valve0"D1'8 +94 "core" ı' +3 ."detached from preston"Carousel.seek 55 +3 "carousel0"F2'4 +14 "core" Ʋ' +4 "carousel0"F3% +3 "core" Ƴ%4XQ[  +3 ."detached from preston"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +4 "syringe1"C4& +3 "core" ô&V +3 ."detached from preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +4 ."detached from preston"Lysis1.seek :locked +2 "syringe1"C5( +247 "core" õ( +4 "valve0"D6% +7 "core" Ķ%8ZO +3 ."Plunge"Plunge.in :default + "valve0"D> P ޤ-2 +4 "syringe1"C7% +5 ."Plunge"Plunge.seek :locked +6 "valve0"D8'Z +4 "core" ÷%ݞ +7 ."Air"Air.in :fast + "syringe1"C> P]z<2 +4 ."Air"Air.seek :locked +2 "syringe1"C9'6 +77 "core" ĸ' +26 ù' +2 ."detached from preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +4 F:% +3 "core" ƺ%4\\QG  +7 #"detached from preston"Powering cartridge bus +265 "cartridge_broadcast" ( +103 ."detached from preston"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +5 ."detached from preston"PuckHeater.configure :default +2 "Cartridge" \ :D H  +5 ."detached from preston"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" ;t +3 "cartridge" t +2 "Cartridge" % +4 "core" ľ%ZGEO2  +3 "syringe1"C?& +3 "core" ÿ& +3 "syringe1"C@% +3 "core" %6 S +3 "carousel0"FA% +4 "core" %4P \ +4 #"FILTERING"Preparing to filter lyseHold_phyto cartridge in slot 55 +2 "valve0"DB% +5 "core" %ZG4O5 ( +2 "syringe1"CC& +6 "core" & +5 "syringe1"CD% +4 "core" %6, ] +4 ."sealing"Air.in :default +3 "syringe1"C>d P]z<2 +3 "carousel0"FE% +6 ."sealing"Air.seek :sealed +2 "syringe1"CF' +4 "core" %4hP \ +3 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml + "sampler0"H7 +3 ."FILTERING"Exhaust.close +2 "sampler0"HG0 +5 "core" 0 +2 ."FILTERING"Bypass.open +2 "sampler1"IH1 +148 "core" 1 +75 "ambient0"JI% +3 "core" %? +3 ."FILTERING"Intake.open +2 "sampler0"HJ1 +15 "core" ' +125 1 +2 "ambient0"JK% +5 "core" %3 +3 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"HL% +3 "core" %@ +48 "sampler0"HM% +4 "core" %R +46 "sampler0"HN% +3 "core" %6 +47 "sampler0"HO% +5 "core" %6x +45 "sampler0"HP% +3 "core" %/t +47 "sampler0"HQ% +4 "core" %1] +4 "Can"j_P,zduF? + #"FILTERING"Vacuum tare is 54% of full scale at 24C (drifted 2%) +2 "ambient0"JR% +5 "core" %, +2 "ambient0"JS% +5 "core" %B +2 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +3 ."FILTERING"Abs.hold +2 "ambient0"JT+ +43 "core" + +3 "sampler0"HU% +5 "core" %nJ +3 "sampler0"HV% +4 "core" %m` +13 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n#(@=3 22Q +4 ."FILTERING"SamplePump.seek 10ml,1:10 +2 "sampler0"HW'6 +61 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(@=3 22Q +1279 "core" ' +2 "sampler0"HX% +5 "core" %6e 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +3 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +4 ."FILTERING"Exhaust.open + "sampler0"HY2 +149 "core" 2 +73 ."closeBypass"Bypass.close +3 "sampler1"IZ/ +148 "core" / +74 "sampler0"H[% +3 "core" %Pl +2 "ambient0"J\\% +5 "core" %^ +2 ."FILTERING"Abs.in :default +2 "ambient0"J>:6 e  +4 ."FILTERING"Abs.hold +2 "ambient0"J]+ +4 "core" + +2 "ambient0"J^% +5 "core" %] +3 "sampler0"H_% +6 "core" %Jp +2 "sampler0"H`% +5 "core" %Hj +5 ."FILTERING"SamplePump.in :start + "sampler0"H>^ }n#(@X=3 22Q +5 ."FILTERING"SamplePump.seek 200ml,13:50 +2 "sampler0"Ha'G +61 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(@X=3 22Q +3381 "Can"h^,zat.ML +11961 j^`,zRt/kL +10864 "core" ' +2 "sampler0"Hb% +5 "core" %Gq 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"Bypass.open +2 "sampler1"Ic1 +149 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"Hd/ +140 "core" / +2 "carousel0"Fe% +5 "core" %4Py \ +5 ."FILTERING"Toroid.in :hold + "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :crack +2 "valve0"Df( +168 "core" ( +77 ."FILTERING"Toroid.seek :cartridge +2 "valve0"Dg(m +206 "core" ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +3 ."FILTERING"Toroid.seek :clear +2 "valve0"Dh( +301 "Can"j^,zRu !7L +70 "core" ( +2 "ambient0"Ji% +3 "core" %Q +5 "Can"j^,zRu  + "ambient0"Jj% +5 "core" %[ +2 "ambient0"Jk% +3 "core" %S +3 #"FILTERING"Pressurizing cartridge to 14psi above 16.1psia +2 .SamplePump.setPosition! 0ml + "sampler0"H7 +4 ."FILTERING"Intake.open +2 "sampler0"Hl1 +139 "core" 1 +3 ."FILTERING"Bypass.close + "sampler1"Im/ +150 "core" / +2 ."FILTERING"Exhaust.close +2 "sampler0"Hn0 +148 "core" 0 +2 "sampler0"Ho% +5 "core" %s +2 "ambient0"Jp% +3 "core" %W +4 ."FILTERING"Abs.in :default + "ambient0"J>:/ e  +6 ."FILTERING"Abs.hold +3 "ambient0"Jq+ +3 "core" + +3 "sampler0"Hr% +5 "core" %n +2 "sampler0"Hs% +6 "core" % +2 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>nu0e 3 22Q +5 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +2 "sampler0"Ht' +1414 "core" '(. 2 +5 "sampler0"Hu% +5 "core" %+ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +5 ."FILTERING"Intake.close +2 "sampler0"Hv/ +146 "core" / +2 "ambient0"Jw% +5 "core" %q +2 #"FILTERING"Pressurized cartridge with 0.848ml to 14.2psi +2 #Isolated @29.5psia (13.4psi) +2 #Verifying cartridge holds at least 0.65..0.85 of 29.5psia +4 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +2 "ambient0"Jx% +4 "core" %m +47 "ambient0"Jy% +3 "core" %b +47 "ambient0"Jz% +3 "core" %X +47 "ambient0"J{% +3 "core" %N +47 "ambient0"J|% +3 "core" %I +47 "ambient0"J}% +3 "core" %B +47 "ambient0"J~% +3 "core" %C +47 "ambient0"J% +4 "core" %C +46 "ambient0"J% +3 "core" ʁ%C +47 "ambient0"J% +3 "core" ʂ%= +3 #"FILTERING"Cartridge pressure fell 2% to 28.8psia +2 "carousel0"F% +47 "core" Ƅ%4P \ +12 #"FILTERING"Sampling 100.0ml +2 .SamplePump.setPosition! 0ml + "sampler0"H7 +5 ."FILTERING"Exhaust.open + "sampler0"H2 +148 "core" ȅ2 +2 ."FILTERING"Intake.open +2 "sampler0"H1 +145 "core" ȇ1 +4 "Can"j^,zRu"b +2 "ambient0"J % +3 "core" ʉ%| +5 "ambient0"J\ % +4 "core" ʊ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +3 ."FILTERING"Abs.hold +2 "ambient0"J + +3 "core" ʋ+ +2 "ambient0"J % +5 "core" ʍ% +3 "sampler0"H% +3 "core" ȏ%n  +4 "sampler0"H% +4 "core" ȑ%  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n#(e@X@ 3 22Q +5 ."FILTERING"SamplePump.seek 100ml,6:04 +2 "sampler0"H'# +60 ."pumpStarted"SamplePump.configure :sample + "sampler0"H#d n#(e@X@ 3 22Q +227 H% +3 "core" ȕ%\ \\ 2 +3 "ambient0"J% +5 "core" ʜ%x +2 #"sampling"Cartridge 55 filtering 2/100ml at 0.74ml/s with 11.8psi vacuum +5993 "sampler0"H% +3 "core" Ț% * 2 +3 "ambient0"J% +5 "core" ʙ% + #"sampling"Cartridge 55 filtering 47/100ml at 0.74ml/s with 6.0psi vacuum 4.3m deep (21.0psia) +2558 "Can"l],zRt/9L +3430 "sampler0"H% +3 "core" Ș%P gi 2 +3 "ambient0"J% +5 "core" ʖ%9 +2 #"sampling"Cartridge 55 filtering 91/100ml at 0.74ml/s with 11.5psi vacuum +1214 "core" ȓ' +2 "sampler0"H% +4 "core" ȗ%#} 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +7 ."closeIntake"Intake.close +4 ."closeExhaust"Exhaust.close +5 "sampler0"H/ +4 H0 +3 "carousel0"F% +7 "core" Ɔ%4iP \ +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :bypass +2 "valve0"D( +119 "core" ț/ +9 ȝ0 +224 Ď( +5 ."FILTERING"Toroid.in :default + "valve0"D? ֜2 +4 ."FILTERING"Toroid.seek :clear +2 "valve0"D( +352 "core" Đ( +4 #"FILTERING"Sampled 100.0ml +2 "carousel0"F#% +5 "core" ƣ%4Pn \ +7 "valve0"D%% +4 "core" ĥ%ZG(O? 4 +3 "syringe1"C&& +3 "core" æ& +3 "syringe1"C'% +3 "core" ç%J +4 "carousel0"F(% +5 "core" ƨ%4P \ +3 "carousel0"F)% +5 "core" Ʃ%4Pp \ +4 ."PROCESSING"Handoff.select :waste +2 "valve0"D +48 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"C+% +5 "core" ë% +46 "syringe1"C,% +3 "core" ì% +47 "syringe1"C-% +3 "core" í% +47 "syringe1"C.% +3 "core" î%~ +47 "syringe1"C/% +3 "core" ï% +47 "syringe1"C0% +3 "core" ð% +47 "syringe1"C% +3 "core" Ò% +47 "syringe1"C % +3 "core" Ì% +48 "syringe1"C3% +3 "core" ó% +47 "syringe1"C4% +4 "core" ô% +46 "syringe1"C% +3 "core" È% +47 "syringe1"C$% +3 "core" ä% +47 "syringe1"C% +3 "core" Ã% +47 "syringe1"C6% +3 "core" ö% +5 "Can"l],zRu2 +2 #"PROCESSING"Syringe tare is 51% of full scale at 25C (drifted -0%) +2 .PinPort.in :locking + "valve1"E? B22 +4 ."PROCESSING"PinPort.seek :locked +2 "valve1"E*( N +78 "core" Ū( +3 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1250uL +3 ."posArchive"Archive.seek :empty +3 "syringe0"B>d P\\k<2 + "syringe1"C7( +2 "syringe0"B ( +8 ."posLyS2"Lysis2.seek 950uL +7 "syringe0"B:' +240 "core" ÷( +258 º' +319  ( +3 "carousel0"F1% +4 "core" Ʊ%4P \ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +3 .PinPort.seek :locked + "valve1"E( N +5 "core" ş( +2 ."PROCESSING"Handoff.select :waste +2 .Plunge.seek :puckBottom +2 "valve0"D;'B +126 "core" Ļ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C<% +3 "core" ü%w +47 "syringe1"C=% +3 "core" ý%~ +47 "syringe1"C>% +3 "core" þ% +47 "syringe1"C?% +3 "core" ÿ%| +47 "syringe1"C@% +3 "core" %z +47 "syringe1"CA% +3 "core" %y +47 "syringe1"CB% +3 "core" %w +47 "syringe1"CC% +3 "core" %{ +48 "syringe1"CD% +3 "core" %t +47 "syringe1"CE% +3 "core" %u +47 "syringe1"CG% +3 "core" %v +47 "syringe1"CH% +3 "core" %x +47 "syringe1"CI% +3 "core" %u +47 "syringe1"C9% +3 "core" ù%w +12 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi +2 #Air stroke #1 of 1 + "syringe1"CJ% +4 "core" %z +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +3 ."PROCESSING"Air.seek :pressurized +2 "syringe1"CK' +515 "core" ' +2 "syringe1"CL% +4 "core" %S < +2 #"PROCESSING"-0.2psi after pressurize +2 .Air.in :compress + "syringe1"C>N  P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"CM'| +651 "core" ' +2 "syringe1"CN% +4 "core" %|K4J }Py +2 #"PROCESSING"0.9psi after compress +2 "syringe1"CO% +3 "core" %|K4 Ph +48 "syringe1"CP% +3 "core" %|4e ^ +47 "syringe1"CQ% +3 "core" %|4i Z +47 "syringe1"CR% +3 "core" %|4f" \\ +47 "syringe1"CS% +3 "core" %|4j t +47 "syringe1"CU% +3 "core" %|4g f +47 "syringe1"CV% +3 "core" %|4g y +47 "syringe1"CX% +3 "core" %|4k j +47 "syringe1"CY% +3 "core" %|4f r +47 "syringe1"CT% +3 "core" %|4j f +47 "syringe1"CZ% +4 "core" %|4h l +46 "syringe1"C[% +3 "core" %|4d m +47 "syringe1"C\\% +3 "core" %|4d d +47 "syringe1"C^% +3 "core" %|4c h +47 "syringe1"C_% +3 "core" %|4f _ +47 "syringe1"C`% +3 "core" %|4f ^ +48 "syringe1"CW% +3 "core" %|4d k +47 "syringe1"Cb% +3 "core" %|4g f +47 "syringe1"Cc% +3 "core" %|4c u +47 "syringe1"Cd% +3 "core" %|4f ^ +47 "syringe1"Ce% +4 "core" %|4d g +46 "syringe1"C]% +3 "core" %|4g b +2 #"PROCESSING"0.1psi after compress (stable) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."PROCESSING"Air.hold +2 "syringe1"C+ +2 "core" Þ+ +4 "syringe1"Cf% +4 "core" %|4c k +2 "syringe1"Cg% +5 "core" %|4b h +2 #"PROCESSING"at -0.0psi Opened to waste + .Handoff.select :blocked +2 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"Di': +82 "core" ' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi + #Air stroke #1 of 8 +2 "syringe1"Cj% +4 "core" %|4t^ k +4 ."PROCESSING"Air.in :compress +3 "syringe1"C>N   P]zP2 +6 ."PROCESSING"Air.seek :evac +2 "syringe1"Ck'| +3 "core" ' +2 "syringe1"Cl% +4 "core" %|4#_ e +2 #"PROCESSING"1.0psi after compress +2 "syringe1"Cm% +3 "core" %|4_p v +48 "syringe1"Cn% +3 "core" %|4X e +47 "syringe1"Co% +3 "core" %|4[ ` +47 "syringe1"Cp% +3 "core" %|4\\ ] +47 "syringe1"Cr% +3 "core" %|4^ t +47 "syringe1"Cs% +4 "core" %|4l h +46 "syringe1"Ca% +3 "core" %|4Y k +47 "syringe1"Cu% +3 "core" %|4` n +47 "syringe1"Cv% +3 "core" %|4c { +47 "syringe1"Cw% +3 "core" %|4\\ g +47 "syringe1"Cx% +3 "core" %|4\\ t +47 "syringe1"Cy% +3 "core" %|4] b +47 "syringe1"Cz% +3 "core" %|4] a +47 "syringe1"Cq% +3 "core" %|4[ h +47 "syringe1"C{% +3 "core" %|4X g +47 "syringe1"C|% +4 "core" %|4U i +47 "syringe1"C}% +3 "core" %|4X k +47 "syringe1"C~% +3 "core" %|4Y r +47 "syringe1"C% +3 "core" %|4Z f +47 "syringe1"C% +3 "core" Á%|4W l +47 "syringe1"C% +3 "core" Â%|4S m +47 "syringe1"C% +44 "core" Ä%|4R u +2 #"PROCESSING"1.6psi after compress (stable) +3 .Air.in :gulp + "syringe1"C>N   P]z<2 +4 ."PROCESSING"Air.seek :topVent +2 "syringe1"C'6 +419 "Can"k],zQt<$}L +216 "core" Å' +2 "syringe1"C% +3 "core" Ç%6Cɪ < +3 #"PROCESSING"1.6psi after gulp + "syringe1"C % +5 "core" É%6Fɤ < +46 "syringe1"C\ % +3 "core" Ê%6ɭ +47 "syringe1"C % +3 "core" Í%6ɱ +47 "syringe1"C% +3 "core" Ï%6ɠ +47 "syringe1"C% +3 "core" Ñ%6ɪ +47 "syringe1"C% +3 "core" Õ%6ɦ +48 "syringe1"C% +4 "core" Ü%6ɦ~ +46 "syringe1"Ct% +3 "core" %6ɰ +47 "syringe1"C% +3 "core" Ú%6ɨ +47 "syringe1"C% +3 "core" Ù%6ɩ +47 "syringe1"C% +3 "core" Ø%6ɭ +47 "syringe1"C% +3 "core" Ö%6ɳ +3 #"PROCESSING"1.7psi after gulp (stable) + #Air stroke #2 of 8 + "syringe1"C% +4 "core" ×%6ɯ +4 ."PROCESSING"Air.in :pressurize + "syringe1"C>@N   P]z<2 +4 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C' +628 "core" Æ' +2 "syringe1"C% +4 "core" Û%S < +2 #"PROCESSING"1.8psi after pressurize +2 .Air.in :compress + "syringe1"C>N   P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +651 "core" Ý' +2 "syringe1"C % +3 "core" Ë%|d4 Pv +3 #"PROCESSING"13.1psi after compress +2 "syringe1"Ch% +3 "core" %|g49 |Pw +48 "syringe1"C% +3 "core" Î%|4v s +46 "syringe1"C#% +3 "core" ã%|4x i +47 "syringe1"C%% +3 "core" å%|4x h +47 "syringe1"C&% +3 "core" æ%|4r- m +47 "syringe1"C'% +3 "core" ç%|4t6 o +47 "syringe1"C(% +3 "core" è%|4y, n +47 "syringe1"C)% +3 "core" é%|4u= o +48 "syringe1"C+% +3 "core" ë%|4pK m +47 "syringe1"C,% +2 "core" ì%|4p\\ j +47 "syringe1"C-% +4 "core" í%|4qf e +47 "syringe1"C.% +2 "core" î%|4ow x +47 "syringe1"C/% +3 "core" ï%|4j p +47 "syringe1"C0% +3 "core" ð%|4t i +47 "syringe1"C% +3 "core" Ò%|4r r +48 "syringe1"C % +3 "core" Ì%|4r n +47 "syringe1"C3% +3 "core" ó%|4o p +47 "syringe1"C4% +3 "core" ô%|4m k +47 "syringe1"C% +3 "core" È%|4s e +47 "syringe1"C$% +3 "core" ä%|4p j +47 "syringe1"C% +4 "core" Ã%|4m r +46 "syringe1"C6% +3 "core" ö%|4m q +47 "syringe1"C% +3 "core" Ô%|4n j +3 #"PROCESSING"15.2psi after compress (stable) +2 .Air.in :gulp + "syringe1"C>N   P]z<2 +4 ."PROCESSING"Air.seek :topVent +2 "syringe1"C5'6 +636 "core" õ' +2 "syringe1"C"% +4 "core" â%6aɭ < +2 #"PROCESSING"15.1psi after gulp +2 "syringe1"C!% +4 "core" á%6eɩ < +47 "syringe1"C1% +3 "core" ñ%6ɳf +47 "syringe1"C<% +3 "core" ü%6ɧd +47 "syringe1"C=% +3 "core" ý%6ɨ` +47 "syringe1"C>% +3 "core" þ%6ɩb +47 "syringe1"C?% +3 "core" ÿ%6ɩd +48 "syringe1"C*% +3 "core" ê%6ɤb +47 "syringe1"C8% +2 "core" ø%6ɡ` +48 "syringe1"C@% +3 "core" %6ɮ` +47 "syringe1"CA% +2 "core" %6ɴ` +48 "syringe1"CB% +4 "core" %6ɮ` +46 "syringe1"CC% +3 "core" %6ɩb +3 #"PROCESSING"15.4psi after gulp (stable) + #Air stroke #3 of 8 + "syringe1"CD% +4 "core" %6ɣ] +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N   P]z<2 +3 ."PROCESSING"Air.seek :pressurized +2 "syringe1"CE' +628 "core" ' +2 "syringe1"CG% +3 "core" %S < +3 #"PROCESSING"15.3psi after pressurize +2 .Air.in :compress + "syringe1"C>N   P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"CH'| +489 "core" 'p d P]z<2 +4 ."PROCESSING"Air.hold +4 "syringe1"C9+ +4 "core" ù+ +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"DJ'B +83 "core" ' +2 ."PROCESSING"delay 3 seconds +303 .Handoff.select :waste +4 "valve0"D +47 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi + "syringe1"CF% +3 "core" %d  P]z<2 +4 ."PROCESSING"Air.seek :fullVent +2 "syringe1"C'݇ +256 "core" ×' +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :pBottom +2 "valve0"D'B +82 "core" ą' + #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +2 .Plunge.seek :locked +2 "valve0"D'Z +126 "core" ě' + ."PROCESSING"Handoff.select :waste +3 "syringe1"C& +4 "core" Æ&^ W +3 ."PROCESSING"Lysis1.in :slow + "syringe1"C?d P[<2 +12 ."PROCESSING"Lysis1.seek 950uL +2 "syringe1"C ( +1403 "core" Ë( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select :blocked +4 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +2 "valve0"Dh': +84 "core" ' +2 "syringe1"C& +4 "core" Î&zyM +3 ."PROCESSING"Lysis1.seek 350uL +2 "syringe1"C#( +2796 "core" ã( +2 ."PROCESSING"delay 3 seconds +302 "syringe1"C%% +3 "core" å%݇/t +4 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 +2 "syringe1"C> wk+ P]z<2 +4 C&% +3 "core" æ%݇/> +3 "syringe1"C'& +5 "core" ç&Gts D +46 ."Airmixer"Air.seek :evac +8 ."PROCESSING"Lysis1.seek 190uL +4 "syringe1"C('| +3 C)( +755 "core" é( +3 è' +3 ."PROCESSING"Air.in :fast +2 "syringe1"C> P]z<2 +4 ."PROCESSING"Air.seek :full +2 "syringe1"C+'6 +645 "core" ë' +3 ."PROCESSING"Lysis1.seek :empty +2 "syringe1"C,( +443 "Can"j]z,z^t:UOL +450 "core" ì( +2 "valve0"D-% +4 "core" ĭ%:7uOH A +3 "syringe1"C.% +4 "core" î%6ɳ( +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @18.8psi +2 .Plunge.seek :puckBottom +2 "valve0"D/'B +83 "core" į' +2 "valve0"D0% +3 "core" İ%B(V - +3 "syringe1"C% +4 "core" Ò%6ɵH +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @15.7psi +2 #Polling Can every 2:00 +52 "Can"k]u,z]uUĢ +2 ."PROCESSING"PuckHeater.configure :default #ambient 24.6C +2 "Cartridge" \ :D H ; +3 ."PROCESSING"PuckHeater.seek 81.6C +4 "Cartridge"  +3 39 +4 "cartridge" 9" +105 "Cartridge" 49 +3 "cartridge" 9!d +104 "Cartridge" 9 +3 "cartridge" 9"d +104 "Cartridge" $9 +4 "cartridge" 9% d +103 "Cartridge" 9 +3 "cartridge" 9)5 d +104 "Cartridge" 69 +3 "cartridge" 9-_d +103 "Cartridge" 9 +5 "cartridge" 91d +104 "Cartridge" 9 +3 "cartridge" 96Ud +104 "Cartridge" "9 +4 "cartridge" 9:d +103 "Cartridge" !9 +3 "cartridge" 9?#d +104 "Cartridge" 19 +3 "cartridge" 9C!'d +104 "Cartridge" <9 +3 "cartridge" 9G:4+d +104 "Cartridge" =9 +3 "cartridge" 9J0d +103 "Cartridge" >9 +4 "cartridge" 9Nv44d +104 "Cartridge" ?9 +3 "cartridge" 9R 48d +103 "Cartridge" *9 +4 "cartridge" 9U;4=d +103 "Cartridge" 89 +4 "cartridge" 9XXAd +103 "Cartridge" @9 +3 "cartridge" 9[GEd +104 "Cartridge" A9 +3 "cartridge" 9^(`Id +104 "Cartridge" B9 +3 "cartridge" 9`4Nd +104 "Cartridge" C9 +3 "cartridge" 9cg`Rd +103 "Cartridge" D9 +3 "cartridge" 9e4Vd +104 "Cartridge" 59 +3 "cartridge" 9h"[d +104 "Cartridge" G9 +4 "cartridge" 9jW_d +104 "Cartridge" E9 +3 "cartridge" 9l`cd +103 "Cartridge" I9 +4 "cartridge" 9nogb +103 "Cartridge" H9 +3 "cartridge" 9p-`ld +104 "Cartridge" i9 +6 "cartridge" 9r&pc +104 "Cartridge" F9 +3 "cartridge" 9std +104 "Cartridge" L9 +3 "cartridge" 9uyc +103 "Cartridge" K9 +3 "cartridge" 9wV}c +104 "Cartridge" N9 +3 "cartridge" 9xb +104 "Cartridge" O9 +3 "cartridge" 9zpb +103 "Cartridge" P9 +4 "cartridge" 9{b +104 "Cartridge" Q9 +3 "cartridge" 9}7a +103 "Cartridge" R9 +4 "cartridge" 9~_ +103 "Cartridge" S9 +3 "cartridge" 9^ +104 "Cartridge" U9 +3 "cartridge" 9] +104 "Cartridge" V9 +4 "cartridge" 9~] +103 "Cartridge" X9 +3 "cartridge" 9R\\ +104 "Cartridge" Y9 +3 "cartridge" 9\\ +104 "Cartridge" T9 +3 "cartridge" 9\\ +105 "Cartridge" Z9 +3 "cartridge" 9$=] +103 "Cartridge" [9 +3 "cartridge" 9\\ +104 "Cartridge" \\9 +3 "cartridge" 9C=\\ +4 ."PROCESSING"PuckHeater.hold 2:00,81.6C +5 "Cartridge" ^  +5 "cartridge"  +6599 "Can"i].,zQt6#L +4 i].,zQt6QM +5377 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +52 "Can"l]9,zSu[ι, +3 ."PROCESSING"PuckHeater.configure :default #ambient 24.6C + "Cartridge" \ :D H @ +4 ."PROCESSING"PuckHeater.seek 80.5C +3 "Cartridge" _  +3 `9 +7 "cartridge" 9Z +6 ."PROCESSING"PuckHeater.hold 2:00,80.5C +5 "Cartridge" W G( +4 "cartridge"  +6486 "Can"j]W,zZt)с=L +4 j]W,zZt)с=M +5493 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +52 "Can"l]],zTuYsӹK9 +2 ."PROCESSING"PuckHeater.configure :default #ambient 24.7C +2 "Cartridge" \ :D H B +4 ."PROCESSING"PuckHeater.hold 8:00,79.4C +5 "Cartridge" b tU +6394 "Can"i]Z,zXu%![L +3 i]Z,zXu%![M +11956 j]c,zQu\ %oڏyL +3 j]c,zQu%ڐyM +11959 k],zQu $L +3 k],zQu$M +11958 j]e,zRu%n=L +3 j]e,zRu%=M +5643 "cartridge" +3 "syringe1"Cc% +4 "core" %6ɨr+ +2 #"PROCESSING"Lysate cooked @16.0psi +2 .Plunge.seek :puckTop +2 "valve0"Dd': +82 "core" ' +12 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi +2 #Air stroke #1 of 5 + "syringe1"Ce% +4 "core" %6ɦrZ +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N u P]z<2 +7 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C]' +628 "core" ' +2 "syringe1"Cf% +4 "core" %S < +2 #"PROCESSING"15.7psi after pressurize +3 .Air.in :compress + "syringe1"C>N u P]zP2 +4 ."PROCESSING"Air.seek :evac +2 "syringe1"Cg'| +506 "core" 'p; |P +5 "syringe1"Cj% +5 "core" %;# uP +2 #"PROCESSING"Reached 40.9 of 40.0psi after pumping 1692uL (0.95 strokes) +2 .Air.in :default + "syringe1"C>d P]z<2 +4 ."PROCESSING"Air.hold +2 "syringe1"Cl+ +3 "core" + +3 ."PROCESSING"delay 2 seconds +203 .Air.in :fast + "syringe1"C> P]z<2 +3 ."PROCESSING"Air.seek :full +2 "syringe1"Cm'6 +617 "core" ' +2 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"Dn'B +83 "core" ' +2 "syringe1"Co& +3 "core" &*%W 7 +4 ."PROCESSING"Lysis1.seek 138uL +2 "syringe1"CM(# +651 "core" ( +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :puckTop +2 "valve0"Dp': +82 "core" ' +2 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"C;( +650 "core" û( +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"Dr'B +82 "core" ' +2 "carousel0"Fs% +5 "core" %4Pp \ +8 "valve0"D% +3 "core" Ğ%B(Of +5 "syringe1"Ca% +6 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d 'sJ P[#2 +3 "core" %6ɲL$ +4 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"Cu( +3 Cv& +4 "core" &@*7L #% +3 "syringe1"Cw& +5 "core" &*B #8 +13 "syringe1"Cx& +3 "core" &+ #( +16 "syringe1"Cy% +3 "core" %6!ɿ +4 "syringe1"Cz& +5 "core" &| -b # +9 "syringe1"Cq& +3 "core" &ݽ .o # +17 "syringe1"C{& +3 "core" & 0 # +12 "syringe1"C|% +3 "core" %6ɵ` +4 "syringe1"C}& +4 "core" &ތ 3; # +14 "syringe1"C~& +3 "core" & 4" # +17 "syringe1"C& +3 "core" &D 6r # +9 "syringe1"C% +3 "core" Á%6ɺ +6 "syringe1"C& +7 "core" Â&߶ 8 # +12 "syringe1"C& +3 "core" Ä& : # +17 "syringe1"Ck& +3 "core" &k Bg # +17 "syringe1"C\ & +3 "core" Ê&- @n # +17 "syringe1"C & +3 "core" Í& Bw # +4 "syringe1"C% +4 "core" Ï%6ɱA +9 "syringe1"C& +3 "core" Ñ& C #4 +17 "syringe1"C& +3 "core" Õ&S*E #7 +17 "syringe1"C& +3 "core" Ü&*Gˋ4 #G +4 "syringe1"Ct% +4 "core" %6ɹ +2 "syringe1"C% +5 "core" Ú%6ɮd +3 ."pump"Air.in :pressurize + "syringe1"C>@N  P]z<2 +5 C& + ."pump"Air.seek :pressurized +4 "syringe1"C' +5 "core" Ù&HJA #L +10 "syringe1"CJ& +3 "core" &$Kʉ #? +10 "syringe1"C9& +3 "core" ù&},LJ̉ #Q +17 "syringe1"C& +3 "core" Æ&,M x #G +17 "syringe1"C& +3 "core" Ö&61MeN #A +17 "syringe1"C& +3 "core" Å&/Mk? #E +17 "syringe1"C7& +3 "core" ÷&MrˉE > +17 "syringe1"C& +4 "core" Î&+MpΉC @ +16 "syringe1"C& +3 "core" Û&'MjɉO F +17 "syringe1"C & +3 "core" Ë&MvɉA : +17 "syringe1"C%& +3 "core" å&,MuɉE ; +18 "syringe1"C&& +5 "core" æ&0Msĉ\\ = +14 "syringe1"C'& +3 "core" ç&'M|ΉM 4 +17 "syringe1"C& +3 "core" ×&+Ms‰W = +17 "syringe1"C#& +3 "core" ã&,MoƉT A +17 "syringe1"C(& +3 "core" è&0MgˉE I +17 "syringe1"C)& +3 "core" é&;Mcy M +17 "syringe1"C-& +3 "core" í&7Mtĉg < +17 "syringe1"C.& +3 "core" î&dN  P]z<2 +3 "core" ñ&MvO : +2 ."pump"Air.seek :evac +3 "syringe1"C<& +3 C='| +6 "core" ü&Mum ; +4 "syringe1"C>& +4 "core" þ&6M{͉ 5 +9 "syringe1"C?& +3 "core" ÿ&8Mlĉ6 D +17 "syringe1"C*& +4 "core" ê&Mq% ? +16 "syringe1"C8& +3 "core" ø&8Moĉ6 A +17 "syringe1"C@& +3 "core" &6Mq8 ? +17 "syringe1"CA& +3 "core" &8Mp, @ +17 "syringe1"CB& +3 "core" &9MqC ? +17 "syringe1"CC& +3 "core" &>Mo$ A +17 "syringe1"CD& +3 "core" & +17 "syringe1"Ci& +3 "core" &CMi\\ G +17 "syringe1"CF& +3 "core" &;Mr^ > +17 "syringe1"CL& +3 "core" &?Mx{ 8 +17 "syringe1"CK& +3 "core" &EMm{ C +17 "syringe1"CN& +3 "core" &EMp @ +17 "syringe1"CO& +3 "core" &!Miʼn~ G +17 "syringe1"CP& +3 "core" &7Mh H +17 "syringe1"CQ& +3 "core" &>Mlʼn D +17 "syringe1"CR& +4 "core" &IMk E +16 "syringe1"CS& +3 "core" &OMqɉ ? +17 "syringe1"CU& +3 "core" &JMl D +17 "syringe1"CV& +3 "core" &IMr > +17 "syringe1"CX& +3 "core" &LMt < +17 "syringe1"CY& +3 "core" &7Ml D +17 "syringe1"CT& +3 "core" &HMpP @ +17 "syringe1"CZ& +3 "core" &?Mu ; +17 "syringe1"C[& +3 "core" &,Mm C +17 "syringe1"C\\& +3 "core" &NMk3 E +17 "syringe1"C^& +3 "core" &GMi2 G +17 "syringe1"C`& +3 "core" &PMiH G +17 "syringe1"CW& +3 "core" &QMph @ +17 "syringe1"C & +3 "core" Ì&OMiX G +17 "syringe1"C_& +3 "core" &7MsS = +17 "syringe1"Cb& +3 "core" &6Mp @ +17 "syringe1"Cc& +3 "core" &LMl D +17 "syringe1"Ce& +4 "core" &XMxŠ 8 +16 "syringe1"C/& +3 "core" ï&OMx 8 +17 "syringe1"C+& +3 "core" ë&QMi G +17 "syringe1"Cf& +3 "core" &VMh H +17 "syringe1"C]& +3 "core" &FMr > +17 "syringe1"Cj& +3 "core" &JMs = +17 "syringe1"Cg& +3 "core" &VMt < +17 "syringe1"Cl& +3 "core" &TMs- = +17 "syringe1"Co& +3 "core" &7MyD 7 +17 "syringe1"Cd& +3 "core" &PMho H +17 "syringe1"C,& +3 "core" ì&XMm C +17 "syringe1"Cn& +4 "core" &[Mu ; +16 "syringe1"CM& +3 "core" &YMq ? +17 "syringe1"Cs& +3 "core" &XMj F +17 "syringe1"C& +3 "core" Þ&LMn B +17 "syringe1"Ca& +3 "core" &7Ms = +17 "syringe1"Cv& +3 "core" &\\Mi[ G +17 "syringe1"Cw& +3 "core" &\ =Mq #? +17 "syringe1"Cx& +3 "core" &RMy쌛 #A +17 "syringe1"Cy& +3 "core" &,jM錿 #; +17 "syringe1"Cz& +3 "core" &HeM팿 #6 +17 "syringe1"Cq& +3 "core" &lfM #3 +17 "syringe1"C{& +4 "core" &aNE #0 +16 "syringe1"C|& +3 "core" &eN #6 +17 "syringe1"C}& +3 "core" &-eO #9 +17 "syringe1"C~& +3 "core" &IRO #* +17 "syringe1"C& +3 "core" &j\\Pg #% +17 "syringe1"C& +3 "core" Á&0eQ) # +4 "Can"h]5,zTtMHL +13 "syringe1"C& +3 "core" Â&7DQc # +17 "syringe1"Cp& +3 "core" &=rRc # +17 "syringe1"C& +3 "core" Ä&DlS~7 # +17 "syringe1"Ck& +3 "core" &*TkTS # +17 "syringe1"C& +3 "core" Ç&UpNU4 # +17 "syringe1"C & +3 "core" É&iV$ # +17 "syringe1"C\ & +3 "core" Ê&MW< # +17 "syringe1"C & +4 "core" Í&-lX勗 # +16 "syringe1"C& +3 "core" Ï&CnZ # +17 "syringe1"C& +4 "core" Ñ&Mx[* # +16 "syringe1"C;& +3 "core" û&n\\ # +17 "syringe1"C& +3 "core" Õ& u^  # +17 "syringe1"C& +3 "core" Ü&9Xx^ # +17 "syringe1"Ct& +3 "core" &_ m_ # +17 "syringe1"C& +3 "core" Ú&{[`? # +17 "syringe1"C& +3 "core" Ù&OK`5 # +17 "syringe1"CJ& +3 "core" &v`` # +17 "syringe1"C9& +3 "core" ù&A` +17 "syringe1"C& +3 "core" Æ&Y` +17 "syringe1"C& +3 "core" Ö&\\` +17 "syringe1"C& +3 "core" Å&_`5 +17 "syringe1"C7& +3 "core" ÷&5` # +17 "syringe1"C& +62 "core" ý'Wx= < +8 "syringe1"C% +5 "core" Î&aC # + Û%W= +3 "syringe1"C & +2 #"pump"Reached 15.9 of 15.5psi after pumping 1665uL (0.94 strokes) +5 .Air.in :default + "syringe1"C>d P]z<2 +3 "core" Ë& a # +2 ."pump"Air.hold +3 "syringe1"C%+ +5 C&& +2 "core" å+ +2 æ&*ba # +4 "syringe1"C'% +6 C& +3 "core" ç%W= +5 ×&Pbs # +4 "syringe1"C#& +2 "core" ( +7 "syringe1"C(& +2 "core" ã&cD # +3 è&cB # +2 "syringe1"Cm% +4 "core" %W= +2 #"PROCESSING"Pulled 675uL lysate @15.9psi +2 "valve0"D)% +7 "core" ĩ%B( O? +5 "syringe1"C-% +6 ."PROCESSING"Lysis1.seek 800uL,nil +3 "syringe1"C.(% +4 "core" í%W=r +4 "syringe1"C0& +4 "core" ð& cK # +4 "syringe1"C& +3 "core" Ò&6cR* # +17 "syringe1"C3& +5 "core" ó& c # +15 "syringe1"C4& +3 "core" ô&4c # +4 "syringe1"Ch% +4 "core" %W=^ +9 "syringe1"C& +3 "core" È&Qd # +17 "syringe1"C$& +3 "core" ä&v<eH # +17 "syringe1"C& +3 "core" Ã&Gf^ # +4 "syringe1"C6% +3 "core" ö%W= +3 "syringe1"C% +5 "core" Ô%W= +3 ."pump"Air.in :compressLysate +2 "syringe1"C>dN  P]z<2 +3 ."pump"Air.seek :evac +2 "syringe1"C'| +5 C"& +2 "core" â&Agv # +5 "syringe1"C!& +3 "core" á&rYg< # +17 "syringe1"C1& +3 "core" ñ&Qh # +17 "syringe1"C<& +3 "core" ü&<IiM # +17 "syringe1"C>& +4 "core" þ&MXCi# # +16 "syringe1"C?& +3 "core" ÿ&TVi= # +17 "syringe1"C*& +3 "core" ê&USif # +17 "syringe1"C8& +3 "core" ø&UHi +17 "syringe1"C@& +3 "core" &UKi +17 "syringe1"CA& +3 "core" &UNi +17 "syringe1"CB& +3 "core" &UKi +17 "syringe1"CC& +3 "core" &UVi +17 "syringe1"CD& +3 "core" &URi +17 "syringe1"C5& +3 "core" õ&UPi& +17 "syringe1"CG& +3 "core" &U6i= +17 "syringe1"CE& +4 "core" &USi # +16 "syringe1"CI& +3 "core" &UFiˌ # +17 "syringe1"CH& +3 "core" &WLiጒ # +17 "syringe1"Ci& +3 "core" &[4ji쌬 # +17 "syringe1"C& +3 "core" Ø&cLaj} # +17 "syringe1"CF& +3 "core" &nhYj_쌖 #z +17 "syringe1"CL& +3 "core" &|`j> # +17 "syringe1"CK& +3 "core" &jjꌒ #} +17 "syringe1"CN& +3 "core" &@kf # +17 "syringe1"CO& +3 "core" &[k쌜 # +17 "syringe1"CP& +3 "core" &gl錤 #n +17 "syringe1"CQ& +3 "core" &lm"ތz #o +17 "syringe1"CR& +3 "core" &im݌ #x +7 î( +2 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +5 ."pump"Air.hold +2 "syringe1"CS+ +3 "core" Ý'm7K匏 d  P]z<2 +4 ."PROCESSING"Air.seek :fullVent +2 "syringe1"CX'݇ +199 "core" ' +2 ."PROCESSING"Handoff.select :waste +2 "valve0"D +50 #"PROCESSING"Recovered 800uL lysate @15.5psi +3 .delay 3 seconds +302 "syringe1"CY% +4 "core" %݇/iR +2 #"PROCESSING"After 3 delay Recovered 800uL lysate @0.9psi +2 "carousel0"FT% +4 "core" %4Px \ +6 "sampler0"HZ% +5 "core" %#| +4 ."closeHandoff"Handoff.select :close +3 "valve0"D0 +48 ."detached from preston"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF + "valve1"E[& +3 "core" & N<O, +3 ."detached from preston"PinPort.in :unlocking +2 "valve1"E? B2 +4 ."detached from preston"PinPort.seek :clear +4 "valve1"E\\( +78 "core" ( +2 ."detached from preston"Carousel.in :default +2 "carousel0"F><` F 22 +3 F^% +3 "core" %4bP_ \ +4 ."detached from preston"Carousel.home.seek 55 +2 "carousel0"F`'4 +14 "core" ' +2 "carousel0"FW% +6 "core" %4WQ5  +2 "syringe1"C & +5 "core" Ì&%n h- k +3 ."detached from preston"Plunge.seek :locked +2 "valve0"D_'Z +126 "core" ' +2 "syringe0"Bb& +4 "core" &*k +5 ."Archive"Archive.in :unlocking +2 "syringe1"Cc% +3 "syringe0"B? PZ-2 +5 "core" %݇/l +3 ."Archive"Archive.seek :clear +5 "syringe1"Ce& +4 ."Air"Air.in :unlocking +4 "syringe0"B/(j + "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +2 "syringe1"C+' +3 "core" &%nk o +57 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +3 "syringe0"Bf% +5 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C]( +4 "core" %{~ i +5 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +4 ."Lysis2"Lysis2.seek :clear +2 "syringe0"Bj'j +273 "core" ' +13 ( +75 ¯( +8 ë' +3 "sampler0"Hg% +3 "core" %#Ή +11 ."detached from preston"Gate.power :analytic3,:OFF +2 .Gate.power :motor,:OFF + .Gate.power :analytic1,:OFF +2 .Gate.power :analytic2,:OFF + .Gate.power :core,:OFF + .Gate.power :main,:OFF +2 #Safely stopped and ready to power off @1785522007.66EDT @EDT1785522099.31 "Can"l]V,zSuL +11962 m],zRuZL +11963 m],zQu-L +11962 j],zTuoKL +11961 l^,zMuiL +11961 n^,zTuL +11959 k_,z_uL +11959 j_p,z\\uL +11963 j_,z^u'L +11962 o_,zQuL +11966 k`Q,zTu= L +11964 k`,zRu ;L +11965 n`,zRuS YL +11966 ka ,zVu wL +11964 ia3,zSui L +11963 ka,zRu L +11959 la,zSu~ L +11960 maj,zTu\ L +11961 maE,zTu\ L +11960 ma=,zTu \ +L +11960 ka2,zRu\ IL +11961 la%,zTu7\ gL +11963 la0,zRu\ L +11963 la7,zSuM\ L +11962 laP,zTu\ L +6585 #"preston"Session Begun +1933 #-> ESP.ready! +6 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +2 .Gate.power :analytic2,:ON,limit to 4000mA +4 .Gate.power :analytic3,:ON,limit to 4000mA +11 #Powering core bus +235 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 Dl +3 "core"  +7 ."preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +8 ."preston"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."preston"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."preston"Abs.configure :default +2 "ambient0"J#: e  +10 ."preston"PhaseSeparator.configure :default +2 "carousel1"G$ +8 ."preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 ."preston"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 ."preston"PinPort.configure :default +2 "valve1"E$ B22 +5 #"preston"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"Do% +5 "core" %G,O. x +2 "valve0"Dd& +7 ."Plunge_home"Plunge.home.jog 0 +10 "carousel0"F,% +4 "syringe0"Bn& +8 "syringe1"CM% +4 "valve0"Ds) +6 "syringe1"C& +3 "syringe0"Ba% +3 "valve1"Ev& +28 "core" &O0  +2 Ƭ%NQ'  + &4_  %ݜd + Þ&K!3 i + %y!3  &*N , + ) +9 "valve0"Dw% +8 ."PinPort_home"PinPort.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +5 ."Air_home"Air.home.jog 0 +6 "valve1"Ex* +3 "syringe1"Cy* +8 "syringe0"Bz* +3 "valve0"Dq* +2 "syringe0"B{) +13 "carousel0"F|) +3 "syringe1"C}) +11 "core" %QG.O/  + * + * + * + * + ) + ) ) +3 "syringe0"B~% +8 "syringe1"C% +4 C& +2 "valve1"E& +6 "valve0"Dp& +2 ."Plunge_home"Plunge.in :homing +6 "syringe0"B& +9 "valve0"D> (2 +19 ."Plunge_home"Plunge.jog 1479 +10 "valve0"Dk) +5 "core" %rّc + Á%ݡ' &M`  + ł&O! + &O! + „&x,g  +8 ."Lysis2_home"Lysis2.in :homing +7 ."Lysis1_home"Lysis1.in :homing +5 ."Archive_home"Archive.in :homing +6 ."PinPort_home"PinPort.in :homing +3 ."Air_home"Air.in :homing +3 ."Toroid_home"Toroid.in :homing +5 "syringe0"B> (2 +4 "syringe1"C? (2 +2 "syringe0"B? -2 +8 "valve1"E?2 +2 "syringe1"C> -2 +6 "valve0"D?2 +7 ."Lysis2_home"Lysis2.jog 1621 +5 ."Lysis1_home"Lysis1.jog 1603 +3 ."Archive_home"Archive.jog 1615 +6 ."PinPort_home"PinPort.jog 1232 +5 ."Air_home"Air.jog 1427 +5 ."Toroid_home"Toroid.jog 1362 +6 "syringe0"B)U +3 "syringe1"C *C +7 "syringe0"B\ *O +3 "syringe1"C) +4 "valve1"E * +7 "valve0"D*R +13 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +9 ."Plunge_home"Plunge.seek :home +3 "valve0"D;' +27 "core" Ļ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +41 "core" ‡) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +8 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"B' +5 "core" É* + Ï) + Š* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 ."Air_home"Air.setRawPosition! 0 +3 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe1"C82 +3 C7 +8 "syringe0"B82 +4 ."Lysis1_home"Lysis1.seek :home +5 ."Air_home"Air.seek :home +6 "syringe1"C( +4 ."Archive_home"Archive.seek :home +8 "syringe1"Ct' +3 "syringe0"B( +62 "core" •' + ō* + ' + Ü( š( +3 ."Lysis2_home"Lysis2.in :default +6 ."Lysis1_home"Lysis1.in :default +3 ."Archive_home"Archive.in :default +4 ."PinPort_home"PinPort.setRawPosition! 3150 +5 "syringe0"B>d P\\k<2 +3 ."Air_home"Air.in :default +6 "syringe1"C?d P[<2 +2 "syringe0"B?d PZ<2 +2 "valve1"E8 N +3 "syringe1"C>d P]z<2 +14 ."PinPort_home"PinPort.seek :home +3 "valve1"E( +158 "core" đ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +4 "valve0"DJ( +7 "core" ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +252 "core" ř( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +4 "valve0"D9( +4 "valve1"E& +6 "core" ņ&¢QQ ^ +6 ."preston"PinPort.in :unlocking + "valve1"E? B2 +7 ."preston"PinPort.seek :clear +3 "valve1"E( +43 "core" Ŗ( +287 Ĺ( +3 ."preston"Air.seek :clear +3 "syringe1"C' +7 "core" Å' +3 ."preston"Lysis1.seek :clear +4 "syringe1"C7( +69 "core" ÷( +3 ."preston"Lysis2.seek :clear +3 "syringe0"B'j +76 "core" ›' +4 ."preston"Archive.seek :clear +5 "syringe0"B(j +73 "core" Ž( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 F % +3 "core" Ƌ%4TQ-  +4 ."preston"Carousel.home.seek 55 +4 "carousel0"F&'4 +17 "core" Ʀ' +2 "valve0"D'% +4 "core" ħ%^O7 !v +3 "valve0"D% +9 "core" ė%^O4 !v +3 "sampler0"H(% +4 "core" Ȩ%4l +3 "valve0"Dm& +5 "core" &O4 % +3 "carousel0"F)% +5 "core" Ʃ%4O{Q*  +3 "ambient0"J-% +3 "core" ʭ%~/ +3 "carousel1"G0& +5 "core" ǰ&NP +2 "syringe0"B& +4 "core" ’&j2Q +3 "syringe1"C3% +5 "core" ó%ݬW +2 "syringe1"C4& +4 "core" ô&fȂ +3 "syringe0"Bh% +5 "core" %j٫O +2 "valve1"E& +3 "core" ň&O3  +1128 "Can"ia,zQuPWu\ L +213 #"preston"-> ESP.shutdown +5 "sampler0"H$% +3 "core" Ȥ%!o +11 ."preston"Gate.power :analytic3,:OFF +3 .Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :analytic2,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +11713 "Can"na,zbu \ L +1660 #"preston"-> MainThread.exit +5 #mEXIT +6 !"preston"o:ThreadError: mesg"killed thread:@timestampu: Time 6:bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +7 #Session Ended @1785525226.25EDT @1785525702.08EDT = =tMAIN =mMAIN_scheduler @EDT1785525703.07 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +61 "Can"l`,zRuH y +305 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +70 #Sea pressure (Abs) tare is 6% of full scale +351 "Can"l`,zRuQ { + l`,zRuQ { + #tPolling Can every 2:00 +52 "Can"k`,zJuR { +161 #t-> slot +490 #-> ESP.ready! +4 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +2 .Gate.power :analytic2,:ON,limit to 4000mA +2 .Gate.power :analytic3,:ON,limit to 4000mA +18 #Powering core bus +266 "valve0"DP +3 D +3 "core" ā +6 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +7 .tSamplePump.configure :default + "sampler0"H#^ #(22 +7 .tToroid.configure :default +2 "valve0"D$ ֜2 +7 .tCarousel.configure :default + "carousel0"F#<` F 22 +7 .tAbs.configure :default + "ambient0"J#  +8 .tPhaseSeparator.configure :default + "carousel1"G$ +7 .tArchive.configure :default + "syringe0"B$d PZ<2 +7 .tAir.configure :default +2 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +3 .tLysis2.configure :default +3 "syringe0"B#d P\\k<2 +6 .tPinPort.configure :default +2 "valve1"E$ B22 +4 #tAll elf controllers running firmware version 4.94 +31 .Gate.power :motor,:ON,limit to 5000mA +324 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +3 !to:AnalyticModule::Unknown: mesg"SPR:bt[ "D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"2/home/esp/esp2/utils/init3g.rb:95:in `ready!'"(ESP):2:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +3 "valve0"D% +3 D& +4 "core" Ă%^lO0 8 +7 "carousel0"F% +4 ."Plunge_home"Plunge.home.jog 0 +12 "syringe0"B& +4 "syringe1"C% +2 "valve0"D) +15 "syringe1"C& +7 "syringe0"B % +2 "valve1"E\ & +19 "core" ă&O)  + Ƅ%NQ  + …&Fc  + Æ%ݢU + ć) È&bc R + ‰%٨  + Ŋ&#O& ,d +8 "valve0"D % +7 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +2 ."Lysis1_home"Lysis1.home.jog 0 +2 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +2 ."PinPort_home"PinPort.home.jog 0 +3 "syringe0"B ) +2 "syringe1"C ) +5 "carousel0"F) +2 "syringe1"C* +3 "syringe0"B* +12 "valve0"D* +3 "valve1"E* +18 "core" ċ%^uO, + Œ) + Í) + Ï* Ǝ) + * + đ* Œ* +3 "syringe0"B% +8 "syringe1"C& +6 "valve1"E& +4 "syringe0"B& +4 ."Plunge_home"Plunge.in :homing +6 "valve0"D& + "syringe1"C% +3 "valve0"D> (2 +61 "core" ”%t٪X + Õ&c + œ&O%  + –&|<X + Ę&O( + ×%ݡ\\  +7 ."Lysis1_home"Lysis1.in :homing +11 ."Plunge_home"Plunge.jog 436 +2 ."Lysis2_home"Lysis2.in :homing +4 ."Air_home"Air.in :homing +2 ."Toroid_home"Toroid.in :homing +3 ."PinPort_home"PinPort.in :homing +2 ."Archive_home"Archive.in :homing +4 "syringe1"C? (2 + "valve0"D) +3 "syringe0"B> (2 +6 "syringe1"C> -2 +2 "valve0"D?2 +4 "valve1"E?2 +6 "syringe0"B? -2 +7 ."Lysis1_home"Lysis1.jog 1599 +7 ."Air_home"Air.jog 1427 +5 ."Lysis2_home"Lysis2.jog 1617 +3 ."PinPort_home"PinPort.jog 1229 +2 ."Toroid_home"Toroid.jog 1363 +4 ."Archive_home"Archive.jog 1613 +6 "syringe1"C*? + C) +2 "syringe0"B)Q +7 "valve1"E* +3 "valve0"D*S +9 "syringe0"B*M +11 "core" ę) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +4 ."Plunge_home"Plunge.seek :home +3 "valve0"D ' +23 "core" Ġ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +48 "core" Û) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +5 ."Air_home"Air.seek :home +4 "syringe1"C!' +3 "core" Ú* + œ) +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +6 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe0"B72 + "syringe1"C82 +8 ."Lysis2_home"Lysis2.seek :home +3 ."Lysis1_home"Lysis1.seek :home +4 "syringe0"B"' +2 "syringe1"C#( +8 "core" á' + Ÿ* +3 ."Archive_home"Archive.setRawPosition! 50 +2 ."Air_home"Air.in :default +4 "syringe0"B82 + "syringe1"C>d P]z<2 +9 ."Archive_home"Archive.seek :home +3 "syringe0"B$( +2 "core" ŝ* +2 ¢' + ã( +4 ."PinPort_home"PinPort.setRawPosition! 3150 +4 ."Lysis2_home"Lysis2.in :default +2 ."Lysis1_home"Lysis1.in :default +3 "valve1"E8 N + "syringe0"B>d P\\k<2 +2 "syringe1"C?d P[<2 +10 ."PinPort_home"PinPort.seek :home +6 "valve1"E%( +7 "core" ¤( +3 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +223 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +3 "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +2 "valve0"D&( +3 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +179 "core" ť( +6 ."PinPort_home"PinPort.in :default +3 "valve1"E? B22 +32 ."clearToroid"Toroid.seek :clear +4 "valve1"E'& +3 "valve0"D(( +4 "core" ŧ&O% q +7 .tPinPort.in :unlocking +2 "valve1"E? B2 +3 .tPinPort.seek :clear +2 "valve1"E)( +41 "core" ũ( +300 Ĩ( +2 .tAir.seek :clear +3 "syringe1"C*' +3 "core" ê' +3 .tLysis1.seek :clear +3 "syringe1"C+( +67 "core" ë( +3 .tLysis2.seek :clear +2 "syringe0"B,'j +74 "core" ¬' +2 .tArchive.seek :clear +3 "syringe0"B-(j +73 "core" ­( +2 .tCarousel.in :default +2 "carousel0"F><` F 22 +5 F.% +2 "core" Ʈ%4TQ  +4 .tCarousel.home.seek 55 +2 "carousel0"F/'4 +19 "core" Ư' +2 "valve0"D0% +4 "core" İ%^O> !Z +3 "valve0"D1% +5 "core" ı%^O8 !_ +3 "sampler0"H2% +5 "core" Ȳ%@f +3 "valve0"D3& +4 "core" ij&O? & +3 "carousel0"F4% +4 "core" ƴ%4VQ3  +3 "ambient0"J5% +3 "core" ʵ%z< +3 "carousel1"G6& +5 "core" Ƕ&WQ& +2 "syringe0"B7& +6 "core" ·&jP +2 "syringe1"C8% +5 "core" ø%ݡD +2 "syringe1"C9& +3 "core" ù&dS +4 "syringe0"B:% +4 "core" º%j٤ +3 "valve1"E;& +3 "core" Ż&1O<  +8755 "Can"k`,zHuI +11962 m`",zPuG +11958 j_,zHuN թ +5777 #t-> ESP.shutdown +3 "sampler0"H<% +3 "core" ȼ%"t +11 .tGate.power :analytic1,:OFF +2 .Gate.power :analytic2,:OFF + .Gate.power :analytic3,:OFF +2 .Gate.power :motor,:OFF + .Gate.power :core,:OFF +2 .Gate.power :main,:OFF +1370 #-> ESP.configure +4 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +2 .Gate.power :analytic2,:ON,limit to 4000mA +2 .Gate.power :analytic3,:ON,limit to 4000mA +8 #Powering core bus +266 "valve0"DP +3 D= +4 "core" Ľ +6 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +7 .tSamplePump.configure :default + "sampler0"H#^ #(22 +7 .tToroid.configure :default +2 "valve0"D$ ֜2 +6 .tCarousel.configure :default + "carousel0"F#<` F 22 +7 .tAbs.configure :default +2 "ambient0"J#  +6 .tPhaseSeparator.configure :default +2 "carousel1"G$ +7 .tArchive.configure :default +2 "syringe0"B$d PZ<2 +6 .tAir.configure :default +2 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +75 .tLysis2.configure :default +2 "syringe0"B#d P\\k<2 +19 .tPinPort.configure :default +2 "valve1"E$ B22 +4 #tAll elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +297 !"Probe-AM1"o:AnalyticModule::Error: mesg"No Response to BREAK:bt[ "0/home/esp/esp2/lib/module.rb:158:in `reset'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +4 !to:AnalyticModule::Unknown: mesg"SPR:bt[ "D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"0/home/esp/esp2/utils/init3g.rb:70:in `each'"D/home/esp/esp2/utils/init3g.rb:70:in `configureAnalyticModules'"6/home/esp/esp2/utils/power3g.rb:71:in `configure'"(ESP):7:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52 +4023 "Can"l_,zQuN< +11965 l_3-zRuN +  +5788 #t-> ESP.shutdown +4 "sampler0"H>% +3 "core" Ⱦ%jZ +11 .tGate.power :analytic1,:OFF +2 .Gate.power :analytic2,:OFF +2 .Gate.power :analytic3,:OFF +2 .Gate.power :motor,:OFF +2 .Gate.power :core,:OFF + .Gate.power :main,:OFF +6149 "Can"kj-zQu / +5105 #t-> ESP.configure +5 .Gate.power :main,:ON,limit to 5000mA + .Gate.power :analytic1,:ON,limit to 4000mA +2 .Gate.power :analytic2,:ON,limit to 4000mA +2 .Gate.power :analytic3,:ON,limit to 4000mA +9 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 D? +3 "core" Ŀ +6 .tPlunge.configure :default +2 "valve0"D# P ޤ-2 +7 .tSamplePump.configure :default +2 "sampler0"H#^ #(22 +6 .tToroid.configure :default +2 "valve0"D$ ֜2 +6 .tCarousel.configure :default +2 "carousel0"F#<` F 22 +7 .tAbs.configure :default + "ambient0"J#  +7 .tPhaseSeparator.configure :default +2 "carousel1"G$ +6 .tArchive.configure :default + "syringe0"B$d PZ<2 +7 .tAir.configure :default +2 "syringe1"C#d P]z<2 +4 .tLysis1.configure :default +2 "syringe1"C$d P[<2 +3 .tLysis2.configure :default +4 "syringe0"B#d P\\k<2 +8 .tPinPort.configure :default +2 "valve1"E$ B22 +4 #tAll elf controllers running firmware version 4.94 +2 .Gate.power :motor,:ON,limit to 5000mA +6476 "Can"k_5-zTuL M +11963 n_<-zTuL k @1785528298.48EDT = =tMAIN =mMAIN_scheduler @EDT1785528299.80 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +153 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +225 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +377 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +370 "Can"op+zHtko) + op+zHtko) + #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"lpm+zGti* +74 #"nadia"Session Begun +174 #-> ESP.ready! +6 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +19 #Powering core bus +224 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +43 "valve0"DP +4 D +2 "core" ā +7 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +8 ."nadia"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +11 ."nadia"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."nadia"Abs.configure :default +2 "ambient0"J#  +8 ."nadia"PhaseSeparator.configure :default +2 "carousel1"G$ +8 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +7 #"nadia"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +5 "valve0"D% +4 "core" Ă%^O2 +5 "valve0"D& +5 ."Plunge_home"Plunge.home.jog 0 +12 "carousel0"F% +3 "syringe0"B& +8 "syringe1"C% +8 "valve0"D) +4 "syringe1"C& +3 "syringe0"B % +7 "valve1"E\ & +14 "core" ă&O5  + Ƅ%N  + …&?h  Ç%ݧ* + Ć) + È&no F + ‰%ٰɁ  + Ŋ&4O2 ,S +8 "valve0"D % +7 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +7 ."Toroid_home"Toroid.home.jog 0 +5 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +5 ."Air_home"Air.home.jog 0 +4 "valve1"E * +3 "syringe1"C * +2 "syringe0"B* +11 "valve0"D* +4 "syringe0"B) + "carousel0"F) +12 "syringe1"C) +7 "core" ċ%^O/  + Ō* +2 Í* + Ž* ď* + ) + Ò) +3 "syringe0"B% +9 "syringe1"C& +7 "syringe0"B& +2 "valve0"D& + "valve1"E& +2 "syringe1"C% +3 ."Plunge_home"Plunge.in :homing +14 "valve0"D> (2 +11 "core" Ƒ) + ”%vٴj + Õ&q؁ + –&y8c + ė&O) + œ&<O! + Ø%ݛc +8 ."Plunge_home"Plunge.jog 429 +9 ."PinPort_home"PinPort.in :homing +5 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +5 ."Archive_home"Archive.in :homing +4 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +3 "valve0"D) +3 "valve1"E?2 +4 "syringe0"B> (2 +2 "syringe1"C> -2 +4 "syringe0"B? -2 +9 "valve0"D?2 +2 "syringe1"C? (2 +5 ."PinPort_home"PinPort.jog 1226 +10 ."Lysis2_home"Lysis2.jog 1615 +4 ."Air_home"Air.jog 1428 +6 ."Archive_home"Archive.jog 1614 +5 ."Toroid_home"Toroid.jog 1360 +4 ."Lysis1_home"Lysis1.jog 1597 +6 "valve1"E* +4 "syringe0"B)O +7 "syringe1"C) +3 "syringe0"B*N +4 "valve0"D*P +4 "syringe1"C*= +5 "core" ę) +3 ."Plunge_home"Plunge.setRawPosition! 50 +3 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"D ' +24 "core" Ġ' +3 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +55 "core" Ü) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +6 ."Air_home"Air.seek :home +5 "syringe1"C!' +4 "core" ›) + * +5 ."Archive_home"Archive.setRawPosition! 50 +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe0"B82 +3 B72 +9 ."Archive_home"Archive.seek :home +6 ."Lysis2_home"Lysis2.seek :home +5 "syringe0"B"( +3 B#' +9 "core" á' + ß* + Ś* +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +43 ."Air_home"Air.in :default +5 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "syringe1"C82 +3 C>d P]z<2 +3 "valve1"E8 N +4 ."Lysis1_home"Lysis1.seek :home +9 ."PinPort_home"PinPort.seek :home +4 "syringe1"C$( +3 "valve1"E%( +17 "core" ¢( + £' +2 ."Archive_home"Archive.in :default +4 ."Lysis2_home"Lysis2.in :default +3 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +11 "core" ä( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +148 "core" Ğ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +5 "core" Ħ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +228 "core" ť( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"E'& +3 "valve0"D(( +10 "core" ŧ&O+ c +6 ."nadia"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."nadia"PinPort.seek :clear +3 "valve1"E)( +42 "core" ũ( +287 Ĩ( +2 ."nadia"Air.seek :clear +3 "syringe1"C*' +7 "core" ê' +3 ."nadia"Lysis1.seek :clear +3 "syringe1"C+( +68 "core" ë( +3 ."nadia"Lysis2.seek :clear +3 "syringe0"B,'j +75 "core" ¬' +4 ."nadia"Archive.seek :clear +4 "syringe0"B-(j +75 "core" ­( +5 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F.% +3 "core" Ʈ%3)WG  +4 ."nadia"Carousel.home.seek 53 +3 "carousel0"F/'2 +34 "core" Ư' +2 "valve0"D0% +5 "core" İ%^OI !T +2 "valve0"D1% +4 "core" ı%^\ OD !a +3 "sampler0"H2% +5 "core" Ȳ%V& +3 "valve0"D3& +4 "core" ij& O? +3 "carousel0"F4% +4 "core" ƴ%2Wk  +3 "ambient0"J5% +4 "core" ʵ%~: +3 "carousel1"G6& +4 "core" Ƕ&Xt +3 "syringe0"B7& +4 "core" ·&j"X +3 "syringe1"C8% +5 "core" ø%ݙZ +2 "syringe1"C9& +5 "core" ù&r~ +2 "syringe0"B:% +3 "core" º%j٤\\ +3 "valve1"E;& +5 "core" Ż&]O=  +1270 #"nadia"-> Power.sampler :ON +4 .Gate.power :sampler,:ON +1073 #-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +7086 "Can"jk,]z3t3#H +10573 #"nadia"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +1391 "Can"jj,zEsD?f +4617 #"nadia"-> 2.times{Sampler.refill};BACsampler.prime 10,10 +3 .Bypass.open +4 "sampler1"I<1 +153 "core" ɼ1 +2 ."nadia"Intake.open +2 "sampler0"H=1 +149 "core" Ƚ1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 H>% +3 "core" Ⱦ%]Z +3 ."nadia"SamplePump.seek 30ml +2 "sampler0"H?'` +3994 "core" ȿ' + ."nadia"Bypass.close +2 "sampler1"I@/ +150 "core" / +2 ."nadia"Exhaust.open +2 "sampler0"HA2 +149 "core" 2 +2 "sampler0"HB% +4 "core" %`i +6 ."nadia"SamplePump.seek 55ml +3 "sampler0"HC'-[ +2704 "Can"lf@,z3s0\\0 +633 "core" ' +2 ."nadia"delay 1 second +103 ."closeIntake"Intake.close +2 "sampler0"HD/ +8 ."closeExhaust"Exhaust.close +6 "carousel0"FE% +4 "sampler0"HF0 +5 "core" %2YD  +130 / +14 0 +2 ."nadia"Bypass.open +2 "sampler1"IG1 +154 "core" 1 +2 ."nadia"Intake.open +2 "sampler0"HH1 +143 "core" 1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 HI% +4 "core" %H +3 ."nadia"SamplePump.seek 30ml +2 "sampler0"HJ'` +1627 ."nadia"SamplePump.hold +14 "sampler0"HK+ +5 "core" 'C\\ m {2 +5 + +3 ."nadia"delay 1 second +104 ."closeIntake"Intake.close +4 "sampler0"HL/ +7 ."closeBypass"Bypass.close +6 "carousel0"FM% +4 "sampler1"IN/ +5 "core" %2ZE  +132 / +21 / +5 !"nadia"o:UserAbort : mesg"+Sample Pump (SP) seek to 30ml Aborted!:@timestampu: Time Ʈ.:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:125:in `refill'"!nadia:5:in `topLevelBinding'"nadia:5:in `times'"!nadia:5:in `topLevelBinding'" BACsampler.prime 10,10 +4 "carousel0"FO% +3 "core" %2XD  +4 #"nadia"Priming sample loop w/10ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."nadia"Bypass.open +2 "sampler1"IP1 +156 "core" 1 +73 "ambient0"JQ% +3 "core" %v +2 ."nadia"Intake.open +2 "sampler0"HR1 +145 "core" 1 +2 "ambient0"JS% +4 "core" %i$ +4 #"nadia"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"HT% +7 "core" %u_ +43 "sampler0"HU% +4 "core" %t< +46 "sampler0"HV% +5 "core" %jQ +52 "sampler0"HW% +3 "core" %kH +40 "sampler0"HX% +3 "core" %eI +47 "sampler0"HY% +3 "core" %`G +5 "Can"lf@,z3sDmS + #"nadia"Vacuum tare is 54% of full scale at 25C (drifted 2%) +2 "ambient0"JZ% +7 "core" %j +3 "ambient0"J[% +4 "core" %S +2 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +5 ."nadia"Abs.hold +2 "ambient0"J\\+ +5 "core" + +3 ."nadia"PhaseSeparator.in :full +2 "carousel1"G? +6 ."nadia"PhaseSeparator.hold +2 "carousel1"G], +5 "core" , +4 "sampler0"H^% +4 "core" %]5 +3 "sampler0"H_% +4 "core" %XJ +5 ."nadia"SamplePump.in :start +3 "sampler0"H>^ }m#(q@3 22Q +7 ."nadia"SamplePump.seek 10ml,1:10 +3 "sampler0"H`'6 +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@3 22Q +1285 "core" ' +2 "sampler0"Ha% +4 "core" %6^ 2 +3 ."nadia"Abs.stop +2 "ambient0"J5 +5 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +5 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."nadia"Exhaust.open +2 "sampler0"Hb2 +145 "core" 2 +33 "Can"ke-!z>sD +41 ."closeBypass"Bypass.close +3 "sampler1"Ic/ +154 "core" / +73 "sampler0"Hd% +3 "core" %: +2 "ambient0"Je% +5 "core" % +2 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +6 ."nadia"Abs.hold +3 "ambient0"Jf+ +4 "core" + +3 ."nadia"PhaseSeparator.hold +3 "carousel1"Gg, +5 "core" , +3 "ambient0"Jh% +5 "core" % +2 "sampler0"Hi% +6 "core" %3 +5 "sampler0"Hj% +4 "core" %4 +5 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }m#(q@23 22Q +8 ."nadia"SamplePump.seek 10ml,1:10 +2 "sampler0"Hk'6 +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@23 22Q +1287 "core" ' +2 "sampler0"Hl% +5 "core" %6P 2 +2 ."nadia"Abs.stop + "ambient0"J5 +6 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +1153 #"nadia"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +3422 #-> Cart.release Cart.reserve +6 !o:Cartridge::Error: mesg"Slot (0) is not in 1..60:@timestampu: Time ߛ8:bt["E/home/esp/esp2/utils/cartridge/carousel.rb:284:in `validateSlot'"@/home/esp/esp2/utils/cartridge/carousel.rb:544:in `release'";/home/esp/esp2/utils/cartridge/carousel.rb:811:in `[]'"B/home/esp/esp2/utils/cartridge/carousel.rb:811:in `decompose'"@/home/esp/esp2/utils/cartridge/carousel.rb:543:in `release'"=/home/esp/esp2/utils/cartridge/carousel.rb:542:in `each'"@/home/esp/esp2/utils/cartridge/carousel.rb:542:in `release'"!nadia:8:in `topLevelBinding'" Cart.release Cart.reserved +336 #-> slots +3234 "Can"ld-Mz;t8@L +6794 #"nadia"-> start {fire archive, lysis} +17 "valve0"Dm% +11 Dn& +5 "core" %^OG !M +2 "carousel0"Fo% +3 "syringe0"Bp& +7 "syringe1"Cq% +6 Cr& +5 "syringe0"Bs% +5 "valve1"Et& +22 "core" &OH + &j*b + %2Ym  + %ݤc &k# + %j٧ + &bOC  +8 ."clearToroid"Toroid.seek :clear +4 "valve1"Eu& +4 "valve0"Dv( +9 "core" &gOG  + ( +3 ."detached from nadia"Air.seek :clear +3 "syringe1"Cw' +6 "core" ' +3 ."detached from nadia"Lysis1.seek :clear +5 "syringe1"Cx( +4 "core" ( +3 ."detached from nadia"Lysis2.seek :clear +2 "syringe0"By'j +6 "core" ' +3 ."detached from nadia"Archive.seek :clear +3 "syringe0"Bz(j +7 "core" ( +3 ."detached from nadia"Carousel.in :default +5 "carousel0"F><` F 22 +6 F{% +3 "core" %2X`  +4 ."detached from nadia"Carousel.home.seek 53 +2 "carousel0"F|'2 +17 "core" ' +2 "valve0"D}% +4 "core" %^OG ![ +5 #"detached from nadia"Selecting Cartridge 50 +2 "valve0"D~% +7 "core" %^OD !C +2 "syringe1"C& +5 "core" &u` +2 "syringe1"C% +5 "core" Á%ݓ +2 "valve1"E& +4 "core" ł&eOE  +3 ."detached from nadia"Carousel.seek 50 +3 "carousel0"F\ '/ +86 "core" Ɗ' +4 "carousel0"F % +4 "core" Ɖ%/-Ax 2 +3 ."detached from nadia"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C& +3 "core" È&uρ +4 ."detached from nadia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +9 ."detached from nadia"Lysis1.seek :locked +3 "syringe1"C( +249 "core" Ç( +3 "valve0"D% +8 "core" ą%^O !N +2 ."Plunge"Plunge.seek :locked +3 "valve0"D'Z +57 "syringe1"C% +7 "core" Ã%ݡ] +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C '6 +30 "core" Ą' +74 Ë' +2 ."detached from nadia"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F% +3 "core" Ɠ%/V?  +6 #"detached from nadia"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from nadia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 + "cartridge_broadcast"~ +11 ."detached from nadia"Gate.power :cartridge,:OFF +5 #Mounted archive Cartridge +8 "valve0"D% +3 "core" Ĕ%ZGiOA +3 "syringe1"C& +4 "core" Õ&#X +2 "syringe1"C% +5 "core" Ø%6X t +6 "carousel0"F% +4 "core" Ɩ%/@8 \ +4 #"FILTERING"Preparing to filter archiveHiBiomass_bac cartridge in slot 50 +3 "valve0"D% +7 "core" ė%ZGkOB +2 "syringe1"C& +4 "core" Æ&| +3 "syringe1"C % +4 "core" Ì%6X v +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +6 "core" ǐ&$ +44 "carousel1"G& +3 "core" ǒ&v +47 "carousel1"G& +3 "core" ǎ&t +47 "carousel1"G& +4 "core" Ǐ&j~ +46 "carousel1"G & +3 "core" Ǎ&g +47 "carousel1"G& +3 "core" Ǚ& +47 "carousel1"G& +3 "core" ǜ&e +47 "carousel1"G& +3 "core" ǝ& +48 "carousel1"G& +3 "core" Ǜ&n +47 "carousel1"G& +6 "core" ǟ& +44 "carousel1"G& +3 "core" ǚ&[ +47 "carousel1"G'& +3 "core" ǧ& +47 "carousel1"G& +3 "core" Ǟ& +47 "carousel1"G&& +3 "core" Ǧ& +4 "Can"ld-Mz;t.!ս + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 25C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +7 .PhaseSeparator.in :filling +2 "carousel1"G?HH +5 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G%, +5 "core" ǥ, +3 ."FILTERING"Gate.power :sampler,:ON +709 "core" ǥ,H +7 #"FILTERING"Head pressure reached 3.22psi in 8 seconds +2 "carousel0"F.% +12 "core" Ʈ%/@G \ +4 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +5 ."FILTERING"Exhaust.close +2 "sampler0"H!0 +149 "core" ȡ0 +3 ."FILTERING"Bypass.open +2 "sampler1"I01 +152 "core" ɰ1 +74 "ambient0"J1% +3 "core" ʱ%" +4 "Can"ld-Mz;s@"gئ +2 "ambient0"J$% +5 "core" ʤ%/ +2 "ambient0"J2% +3 "core" ʲ%" +3 ."FILTERING"Abs.in :default +3 "ambient0"J>9 e  +5 ."FILTERING"Abs.hold +2 "ambient0"J3+ +5 "core" ʳ+ +3 ."FILTERING"PhaseSeparator.in :full + "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G4, +5 "core" Ǵ, +5 "sampler0"H5% +3 "core" ȵ%B +3 "sampler0"H6% +4 "core" ȶ%.9 +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "16:17:37.55" +2080 "Can"lcR-kz2s@# +2724 lcR-kz2s@%| +4 ."FILTERING"SamplePump.in :start +3 "sampler0"H>^ }m#(q@3 22Q +7 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"H7'6 +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@3 22Q +1285 "core" ȷ' +3 "sampler0"H8% +4 "core" ȸ%6\\ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +4 ."FILTERING"SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"H92 +152 "core" ȹ2 +73 ."closeBypass"Bypass.close +3 "sampler1"I:/ +148 "core" ɺ/ +73 "sampler0"H;% +3 "core" Ȼ%` +3 "ambient0"J#% +4 "core" ʣ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +7 ."FILTERING"Abs.hold +2 "ambient0"J"+ +9 "core" ʢ+ +4 ."FILTERING"PhaseSeparator.hold + "carousel1"G, +8 "core" Ǒ, +2 "ambient0"J<% +5 "core" ʼ% +2 "sampler0"H=% +6 "core" Ƚ%] +2 "sampler0"H>% +4 "core" Ⱦ%a +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(q@23 22Q +59 ."FILTERING"SamplePump.seek 200ml,13:50 +3 "sampler0"H@'G +8 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }m#(q@23 22Q +7252 "Can"kbl-z2sIW-K +11962 mc-z;sJW7 +7024 "core" ' +2 "sampler0"HA% +5 "core" %G 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"IB1 +150 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"H?/ +147 "core" ȿ/ +2 "carousel0"FE% +4 "core" %/?FB \ +7 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +2 "valve0"DD( +171 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"DF(m +208 "core" ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"DG( +371 "core" ( +3 "ambient0"JH% +3 "core" %^ +4 "Can"mc-z;sgJp>. +2 "ambient0"JI% +4 "core" %h% +3 "ambient0"JC% +4 "core" % +3 #"FILTERING"Pressurizing cartridge to 26psi above 15.2psia +2 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +5 ."FILTERING"Intake.open +2 "sampler0"HM1 +143 "core" 1 +2 ."FILTERING"Bypass.close +2 "sampler1"IL/ +149 "core" / +2 ."FILTERING"Exhaust.close +2 "sampler0"HJ0 +154 "core" 0 +3 "sampler0"HN% +5 "core" %: +2 "ambient0"JO% +4 "core" % +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"JP+ +5 "core" + +4 ."FILTERING"PhaseSeparator.hold + "carousel1"GQ, +8 "core" , +3 "sampler0"HR% +5 "core" % +2 "sampler0"HS% +5 "core" % +3 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>mu0K3 22Q +7 ."FILTERING"SamplePump.seek 2.3ml,40 seconds +3 "sampler0"HT' +1666 "core" 'j 2 +4 "sampler0"HU% +9 "core" %jy- 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"Intake.close +3 "sampler0"HV/ +147 "core" / +2 "ambient0"JW% +4 "core" % +2 #"FILTERING"Pressurized cartridge with 1.171ml to 26.2psi +2 #Isolated @34.9psia (19.7psi) +6 #Verifying cartridge holds at least 0.65..0.85 of 34.9psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +9 "ambient0"JX% +4 "core" % +44 "ambient0"JY% +4 "core" % +46 "ambient0"JZ% +4 "core" % +46 "ambient0"J[% +4 "core" %j +46 "ambient0"J^% +4 "core" %F +46 "ambient0"J_% +4 "core" % +46 "ambient0"JK% +5 "core" % +45 "ambient0"Ja% +4 "core" % +46 "ambient0"Jb% +4 "core" %~ +46 "ambient0"J\\% +4 "core" % +47 "ambient0"J]% +3 "core" % +47 "ambient0"Jc% +3 "core" %n +47 "ambient0"Jd% +3 "core" %a +47 "ambient0"Je% +3 "core" %xW +47 "ambient0"Jh% +3 "core" %z@ +47 "ambient0"Ji% +3 "core" %{/ +47 "ambient0"Jj% +6 "core" %} +2 #"FILTERING"Cartridge pressure fell 19% to 28.1psia +6 "carousel0"F`% +4 "core" %/?C \ +7 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :bypass +3 "valve0"Dl( +355 "core" ( +8 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"Df( +75 "Can"je-z?sdQAAj8 +279 "core" ( +3 "carousel0"Fg% +5 "core" %/>BZ \ +6 !"FILTERING"o:%Cartridge::Sampler::PossibleLeak : mesg"Retained only 28.1psia:@timestampu: Time q\\:@checkpoint: omitted:bt["B/home/esp/esp2/utils/cartridge/deep.rb:197:in `checkMinPress'";/home/esp/esp2/utils/cartridge/deep.rb:230:in `charge'"E/home/esp/esp2/utils/cartridge/sampler.rb:458:in `pressureCheck'">/home/esp/esp2/utils/cartridge/sampler.rb:423:in `filter'"A/home/esp/esp2/type/auv/preconfig.rb:86:in `filterCartridge'"=/home/esp/esp2/utils/auv/cmd.rb:378:in `startFiltering!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"=/home/esp/esp2/utils/auv/cmd.rb:349:in `startFiltering!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" +287 ."sourcePumpShutdown"Gate.power :sampler,:OFF +1870 #"nadia"-> SC +2 "carousel0"Fm% +51 "core" %/@4 \ +866 #"nadia"-> slot 50=>:dry +864 #-> Cart.unlock +5 "valve1"Et& +3 "core" &`OW  +3 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 Fs% +3 "core" %/@^ \ +4 ."nadia"Carousel.home.seek 50 +2 "carousel0"Fr'/ +17 "core" ' +2 "carousel0"Fq% +4 "core" %/7? 2 +3 "syringe0"Bp& +3 "core" &jp +3 "syringe1"Co% +5 "core" %6Ё o +16 ."Air"Air.in :unlocking +4 "syringe0"Bn% +3 "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +5 "syringe1"Cu' +5 "core" %j٠b +88 ' +3 "syringe1"C{& +4 "core" & +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"D}% +4 "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C~( +5 "core" %ZGsOO +5 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +2 "valve0"D'8 +97 "core" ' +13 ( +4262 #"nadia"-> Thread["detached in nadia"].abort +16 !o:NoMethodError\ : mesgu:NameError::message3private method `abort' called for nil:NilClass:@timestampu: Time [Pb: name:\ abort: args[:bt[""nadia:15:in `topLevelBinding'" slots +880 #-> start {fire archive, lysis} +19 #"detached from nadia#2"Selecting Cartridge 50 +5 "valve0"D% +6 "core" ā%8[#OI +7 "syringe1"C& +7 "core" Â&dkx +3 "syringe1"C % +4 "core" É%ݞg +2 "valve1"E& +4 "core" ň&XOJ  +4 ."detached from nadia#2"Carousel.seek 50 +3 "carousel0"F('/ +16 "core" ƨ' +4 "carousel0"F*% +3 "core" ƪ%/Z>  +3 ."detached from nadia#2"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C+& +3 "core" ë&ef +4 ."detached from nadia#2"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from nadia#2"Lysis1.seek :locked +3 "syringe1"C,( +248 "core" ì( +3 "valve0"D-% +6 "core" ĭ%8[O +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +5 "syringe1"C/% +3 "valve0"D|'Z +6 "core" ï%ݟm +5 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"C'6 +78 "core" ' +26 Å' +4 ."detached from nadia#2"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F% +3 "core" ƃ%/_?4i  +7 #"detached from nadia#2"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."detached from nadia#2"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +10 ."detached from nadia#2"Gate.power :cartridge,:OFF +8 #Mounted archive Cartridge +8 "valve0"Dx% +4 "core" %ZGoO\\ +3 "syringe1"C& +4 "core" Ó&"_ +3 "syringe1"C % +4 "core" à%6d h +2 "carousel0"Fw% +4 "core" %/?8 \ +6 #"FILTERING"Preparing to filter archiveHiBiomass_bac cartridge in slot 50 +3 !"detached from nadia"o:Cmd::Error: mesg"(Cannot process while in READY state:@timestampu: Time Yi:bt[">/home/esp/esp2/utils/auv/cmd.rb:467:in `startProcessing!'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'""nadia:11:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6 "valve0"D% +3 #"detached from nadia"Waiting to Resume from Checkpoint "Selecting Cartridge 50" +5 "core" Ĕ%ZGj\ O\\ +3 "syringe1"C& +3 "core" Õ&b +3 "syringe1"C% +4 "core" Ø%6 v +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +8 "core" ǖ& +42 "carousel1"G& +3 "core" Ǘ& +47 "carousel1"G& +3 "core" dž&m +47 "carousel1"G & +4 "core" nj& +46 "carousel1"G& +3 "core" ǐ&= +47 "carousel1"G& +3 "core" ǒ& +47 "carousel1"G& +3 "core" ǎ& +47 "carousel1"G& +4 "core" Ǐ& +47 "carousel1"G & +3 "core" Ǎ& +47 "carousel1"G& +4 "core" Ǚ& +46 "carousel1"G& +3 "core" ǜ&7 +47 "carousel1"G& +3 "core" ǝ&$ +47 "carousel1"G& +4 "core" Ǜ&v +46 "carousel1"G& +4 "core" ǟ& +4 "Can"ldi-z2tHqlT + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 27C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +7 .PhaseSeparator.in :filling + "carousel1"G? +5 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +5 "core" ǚ, +3 ."FILTERING"Gate.power :sampler,:ON +716 "core" ǚ,F +6 #"FILTERING"Head pressure reached 3.14psi in 8 seconds +2 "carousel0"F'% +8 "core" Ƨ%/?F \ +3 #"FILTERING"Priming sample loop w/20ml +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 ."FILTERING"Bypass.open +2 "sampler1"I1 +205 "core" ɞ1 +73 "ambient0"J&% +4 "core" ʦ% +4 "Can"ldi-z2sxGtI2n +3 "ambient0"J.% +8 "core" ʮ% +2 ."FILTERING"Exhaust.open +3 "sampler0"H!2 +148 "core" ȡ2 +74 ."closeBypass"Bypass.close +3 ."FILTERING"Intake.open +6 "sampler1"I0/ +3 "sampler0"H11 +145 "core" ȱ1 +4 ɰ/ +73 "sampler0"H$% +4 "core" Ȥ%  +2 "ambient0"J2% +4 "core" ʲ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J5+ +6 "core" ʵ+ +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?: +7 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G6, +6 "core" Ƕ, +3 "ambient0"J%% +4 "core" ʥ% +4 "sampler0"Hk% +5 "core" %s  +2 "sampler0"H8% +4 "core" ȸ%y  +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "16:27:37.95" +1467 "Can"qe2-z3sFJt +2958 qe2-z3suF}L{s +4 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(q@23 22Q +7 ."FILTERING"SamplePump.seek 20ml,1:50 +2 "sampler0"H9'm +58 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@23 22Q +2596 "core" ȹ' +2 "sampler0"H3% +5 "core" ȳ%mv 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"I41 +150 "core" ɴ1 +2 ."FILTERING"Intake.close + "sampler0"H:/ +147 "core" Ⱥ/ +2 "carousel0"F;% +5 "core" ƻ%/9F \  +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +2 "valve0"D#( +170 "core" ģ( +77 ."FILTERING"Toroid.seek :cartridge +2 "valve0"D<(m +209 "core" ļ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"D=( +371 "core" Ľ( +3 "ambient0"J>% +3 "core" ʾ% +4 "Can"qe2-z3sjJdO- +2 "ambient0"J7% +5 "core" ʷ% +2 "ambient0"JA% +4 "core" %q +3 #"FILTERING"Pressurizing cartridge to 26psi above 15.0psia +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Intake.open +2 "sampler0"HB1 +143 "core" 1 +3 ."FILTERING"Bypass.close +2 "sampler1"I"/ +149 "core" ɢ/ +2 ."FILTERING"Exhaust.close +2 "sampler0"H0 +149 "core" ȑ0 +2 "sampler0"H?% +4 "core" ȿ%؉ +3 "ambient0"JE% +4 "core" %v +3 ."FILTERING"Abs.in :default + "ambient0"J>9 e  +7 ."FILTERING"Abs.hold + "ambient0"Jv+ +6 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GD, +6 "core" , +3 "sampler0"HF% +4 "core" %Љ +3 "sampler0"HH% +5 "core" %Q +3 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>mu0K3 22Q +7 ."FILTERING"SamplePump.seek 2.3ml,40 seconds +4 "sampler0"HI' +1109 "core" '2 2 +4 "sampler0"HC% +4 "core" %0 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"Intake.close +2 "sampler0"HM/ +147 "core" / +3 "ambient0"JL% +4 "core" % +3 #"FILTERING"Pressurized cartridge with 0.514ml to 27.1psi +2 #Isolated @38.2psia (23.1psi) +3 #Verifying cartridge holds at least 0.65..0.85 of 38.2psia +7 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +4 "ambient0"JJ% +6 "core" % +45 "ambient0"JN% +3 "core" % +47 "ambient0"JO% +3 "core" % +47 "ambient0"JR% +3 "core" % +47 "ambient0"JS% +3 "core" % +47 "ambient0"J@% +3 "core" %v +47 "ambient0"JU% +4 "core" %e +46 "ambient0"JV% +4 "core" %J +46 "ambient0"JW% +3 "core" %- +54 "ambient0"JX% +10 "core" % +41 "ambient0"JY% +4 "core" % +46 "ambient0"JZ% +4 "core" % +46 "ambient0"JP% +4 "core" % +46 "ambient0"JQ% +4 "core" % +46 "ambient0"J[% +4 "core" % +46 "ambient0"J^% +4 "core" % +2 #"FILTERING"Cartridge pressure fell 12% to 33.5psia +2 "carousel0"F_% +8 "core" %/:F^ \  +3 #"FILTERING"Sampling 1000.0ml +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +7 ."FILTERING"Exhaust.open +2 "sampler0"HK2 +150 "core" 2 +3 ."FILTERING"Intake.open +2 "sampler0"Ha1 +146 "core" 1 +4 "Can"qe2-z3s\\LQ +2 "ambient0"Jb% +5 "core" %7 +4 "ambient0"J\\% +4 "core" %E +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"J]+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gc, +7 "core" , +2 "ambient0"Jd% +4 "core" %+ +4 "sampler0"He% +5 "core" %9  +2 "sampler0"Hh% +5 "core" %F  +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ m#(@2@K3 22Q +7 ."FILTERING"SamplePump.seek 1000ml,1:07:10 +2 "sampler0"Hi'g+ +58 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d m#(@2@K3 22Q +226 Hj% +3 "core" %\ pY 2 +3 "ambient0"J`% +4 "core" %( { +2 #"sampling"Cartridge 50 filtering 2/1000ml at 0.76ml/s with 12.9psi vacuum +1787 "Can"kem-z5sLWS +4207 "sampler0"HG% +3 "core" % 'nM 2 +3 "ambient0"Jl% +4 "core" % { +2 #"sampling"Cartridge 50 filtering 48/1000ml at 0.76ml/s with 16.6psi vacuum +5987 "sampler0"Hg% +3 "core" % /ϙC }2 +3 "ambient0"Jm% +4 "core" %{ | +2 #"sampling"Cartridge 50 filtering 93/1000ml at 0.76ml/s with 20.4psi vacuum +1745 "Can"kcD.z2sKW4^& +4244 "sampler0"Ht% +3 "core" %R 6y |2 +3 "ambient0"Js% +4 "core" %y { +2 #"sampling"Cartridge 50 filtering 139/1000ml at 0.76ml/s with 22.7psi vacuum +5988 "sampler0"Hq% +3 "core" % 6; 2 +3 "ambient0"Jp% +4 "core" %}? { +2 #"sampling"Cartridge 50 filtering 184/1000ml at 0.75ml/s with 12.7psi vacuum 5.4m deep (22.6psia) +1707 "Can"kaz. z2sIWhk +3623 "core" 'm u0m[ ~2 +5 "sampler0"Ho% +4 "core" %v 2 +3 ."FILTERING"SamplePump.stop +2 "sampler0"H5 +7 ."closeIntake"Intake.close +4 "sampler0"Hn/ +8 ."closeExhaust"Exhaust.close +5 "carousel0"F{% +5 "sampler0"H}0 +7 "core" %/:F \  +8 ."FILTERING"Toroid.in :hold +3 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +4 "valve0"D\ ( +105 "core" / +20 0 +233 Ċ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :clear +2 "valve0"D ( +210 "sampler0"H% +3 "core" ȇ%vzm +3 "ambient0"J% +4 "core" ʄ% +3 #"sampling"Cartridge 50 filtering 223/1000ml at 0.66ml/s with 0.2psi vacuum +131 "core" ċ( +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +11 "carousel0"F% +4 "core" Ɓ%/:Dr \  +4 "carousel0"F% +4 "core" Ƃ%/:E \  +7 "carousel0"F % +5 "core" Ɖ%/:D \  +6 !"FILTERING"o:Cartridge::Sampler::Leaky : mesg"NSerious Leak or Stall -- 0.69ml/s made only -35.7psi vacuum after 223.5ml:@timestampu: Time >:@checkpoint: omitted:bt["=/home/esp/esp2/utils/cartridge/sampler.rb:522:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"E/home/esp/esp2/utils/cartridge/sampler.rb:391:in `sampleQuietly'"7/home/esp/esp2/utils/gensampler.rb:223:in `sample'">/home/esp/esp2/utils/cartridge/sampler.rb:427:in `filter'"A/home/esp/esp2/type/auv/preconfig.rb:86:in `filterCartridge'"=/home/esp/esp2/utils/auv/cmd.rb:378:in `startFiltering!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"=/home/esp/esp2/utils/auv/cmd.rb:349:in `startFiltering!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 #Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 223.47ml" +285 ."sourcePumpShutdown"Gate.power :sampler,:OFF +7194 "Can"l`R.zDtLm +11532 #"nadia"-> TV.bypass +3 "carousel0"F% +8 "core" ƈ%/9"` \  +5 ."nadia"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."nadia"Toroid.seek :bypass +4 "valve0"D*( +353 "core" Ī( +5 ."nadia"Toroid.in :default +2 "valve0"D? ֜2 +6 ."nadia"Toroid.seek :clear +3 "valve0"D+( +31 "Can"k`.zUt# Fp\  +322 "core" ī( +3 "valve0"Dr& +3 "core" &ゕRY  +2140 #"nadia"-> BACsampler.prime 50,nil +2 "carousel0"F-% +5 "core" ƭ%/9 \  +3 #"nadia"Priming sample loop w/50ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +5 ."nadia"Bypass.open +2 "sampler1"I/1 +151 "core" ɯ1 +73 "ambient0"J~% +3 "core" % +4 "Can"k`.zUt32qM +2 "ambient0"J% +4 "core" ʃ% +3 ."nadia"Exhaust.open +2 "sampler0"H2 +149 "core" 2 +74 ."closeBypass"Bypass.close +3 ."nadia"Intake.open +6 "sampler1"Iu/ +2 "sampler0"Hx1 +146 "core" / +65 1 +75 "sampler0"H% +3 "core" ȓ%d/ +3 "ambient0"J % +5 "core" ʠ% +3 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +7 ."nadia"Abs.hold +2 "ambient0"Jw+ +5 "core" + +4 ."nadia"PhaseSeparator.hold + "carousel1"G, +7 "core" ǔ, +3 "ambient0"J% +6 "core" ʕ% +3 "sampler0"H% +4 "core" Ș%[< +2 "sampler0"H% +5 "core" Ȗ%\\ + ǔ,| +4 ."nadia"SamplePump.stop +2 "sampler0"H5 +5 "carousel1"G& +5 "core" Ǘ&n +3 !"nadia"o:)Cartridge::Sampler::LostHeadPressure: mesg""Head Pressure Fell to 0.02psi:bt["?/home/esp/esp2/utils/cartridge/sampler.rb:698:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'""nadia:19:in `topLevelBinding'"^ }m#(q@23 22Q +8 ."nadia"SamplePump.seek 50ml,3:50 +2 "sampler0"H ' +53 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@23 22Q +437 "core" ȍ'F &l }2 +6 "sampler0"H% +4 "core" ș%Q 2 +2 ."nadia"SamplePump.stop +2 "sampler0"H5 +7 ."closeIntake"Intake.close +3 "sampler0"H/ +7 ."closeExhaust"Exhaust.close +9 "carousel0"F% +4 "sampler0"H0 +7 "core" Ɲ%/:G \  +120 Ȝ/ +21 ț0 +3 ."nadia"Abs.stop +2 "ambient0"J5 +7 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +7 !"nadia"o:Cartridge::Sampler::Leaky : mesg"LSerious Leak or Stall -- 0.76ml/s made only -36.1psi vacuum after 3.5ml:@timestampu: Time o|:@checkpoint: omitted:bt["=/home/esp/esp2/utils/cartridge/sampler.rb:522:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'""nadia:19:in `topLevelBinding'" 2.times{Sampler.refill} +3 .Bypass.open +3 "sampler1"I1 +150 "core" ɟ1 +2 ."nadia"Intake.open +2 "sampler0"H'1 +105 "Can"o_.z4sDv( +40 "core" ȧ1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 H% +3 "core" Ȟ%Z +3 ."nadia"SamplePump.in :default +2 "sampler0"H>^ #(22 +7 ."nadia"SamplePump.seek 30ml +3 "sampler0"H&'` +3990 "core" Ȧ' + ."nadia"Bypass.close +2 "sampler1"I./ +151 "core" ɮ/ +2 ."nadia"Exhaust.open +2 "sampler0"H!2 +151 "core" ȡ2 +2 "sampler0"H1% +5 "core" ȱ%`J +3 ."nadia"SamplePump.seek 55ml +3 "sampler0"H0'-[ +3335 "core" Ȱ' +2 ."nadia"delay 1 second +104 ."closeIntake"Intake.close +5 "sampler0"H$/ +7 ."closeExhaust"Exhaust.close +4 "carousel0"F2% +3 "sampler0"H%0 +5 "core" Ʋ%/9E \  +129 Ȥ/ +22 ȥ0 +3 ."nadia"Bypass.open +2 "sampler1"Ik1 +151 "core" 1 +2 ."nadia"Intake.open +3 "sampler0"H81 +144 "core" ȸ1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 H% +3 "core" Ț%X +3 ."nadia"SamplePump.seek 30ml +3 "sampler0"HT'` +917 ."nadia"SamplePump.hold +6 "sampler0"H3+ +7 "core" '% * ~2 +6 ȳ+ +4 ."nadia"delay 1 second +103 ."closeIntake"Intake.close +6 ."closeBypass"Bypass.close +6 "sampler0"H4/ +5 "carousel0"F5% +6 "sampler1"I6/ +4 "core" Ƶ%/:E^ \  +129 ȴ/ +23 ɶ/ +3 !"nadia"o:UserAbort : mesg"+Sample Pump (SP) seek to 30ml Aborted!:@timestampu: Time :@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:125:in `refill'""nadia:20:in `topLevelBinding'"nadia:20:in `times'""nadia:20:in `topLevelBinding'" BACsampler.prime 10,10 +4 "carousel0"F:% +13 "core" ƺ%/9E \  +7 #"nadia"Priming sample loop w/10ml, bypass w/10ml +4 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +10 ."nadia"Bypass.open +2 "sampler1"I;1 +151 "core" ɻ1 +74 "ambient0"Jf% +4 "core" % +5 "Can"o_.z4s@\\B1 +2 "ambient0"J#% +4 "core" ʣ% +2 ."nadia"Intake.open +2 "sampler0"H<1 +144 "core" ȼ1 +73 "sampler0"H>% +4 "core" Ⱦ%f +3 "ambient0"J7% +3 "core" ʷ% +3 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +6 ."nadia"Abs.hold + "ambient0"JA+ +6 "core" + +2 ."nadia"PhaseSeparator.hold +2 "carousel1"GB, +7 "core" , +3 "sampler0"H"% +3 "core" Ȣ%` +3 "sampler0"H% +50 "core" ȑ% +20 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }m#(q@3 22Q +8 ."nadia"SamplePump.seek 10ml,1:10 +3 "sampler0"H?'6 +57 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }m#(q@3 22Q +1115 "Can"l^.z?sJXF +170 "core" ȿ' +2 "sampler0"HE% +5 "core" %6 2 +2 ."nadia"Abs.stop +2 "ambient0"J5 +6 ."nadia"PhaseSeparator.stop + "carousel1"G6 +6 ."nadia"SamplePump.setPosition! 0ml + "sampler0"H7 +6 ."nadia"Exhaust.open +2 "sampler0"HF2 +149 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"IH/ +148 "core" / +73 "sampler0"H9% +4 "core" ȹ%= +6 "ambient0"JC% +4 "core" % +3 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +5 ."nadia"Abs.hold +2 "ambient0"JM+ +6 "core" + +3 ."nadia"PhaseSeparator.hold +2 "carousel1"GL, +7 "core" , +2 "ambient0"JJ% +3 "core" % +4 "sampler0"HN% +4 "core" %; +3 "sampler0"HO% +4 "core" %9 +5 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }m#(q@23 22Q +7 ."nadia"SamplePump.seek 10ml,1:10 +2 "sampler0"Hv'6 +58 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@23 22Q +1284 "core" ' +2 "sampler0"HD% +4 "core" %6 2 +3 ."nadia"Abs.stop + "ambient0"J5 +5 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +9869 "Can"j`(.zOs>dd +11965 ia.z1s> +2857 #"nadia"-> Cart.unlock +5 "valve1"ER& +3 "core" &lOW  +3 ."nadia"Carousel.in :default + "carousel0"F><` F 22 +6 FS% +2 "core" %/::D \  +4 ."nadia"Carousel.home.seek 50 +4 "carousel0"F@'/ +15 "core" ' +3 "carousel0"FU% +4 "core" %/9@rC= 2 +3 "syringe0"BV& +4 "core" &ji\\ +2 "syringe1"CW% +5 "core" %6ԁ v +4 ."Air"Air.in :unlocking +4 "syringe0"BX% +7 "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +3 "syringe1"CY' +5 "core" %j٩_p +91 ' +2 "syringe1"CZ& +4 "core" &ķt +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"DP% +4 "syringe1"C? P[(2 +8 ."Lysis1"Lysis1.seek :clear +3 "syringe1"CQ( +5 "core" %ZGiOH +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +5 ."Plunge"Plunge.seek :clear +3 "valve0"D['8 +96 "core" ' +14 ( +5783 #"nadia"-> slots +1872 #-> slot 51=>[:dry,:archiveHiBiomass_bac] +1107 "Can"n^%.z4sA{ +9440 #"Benjamin"Session Begun +2521 "Can"k^.z2sA! +4643 #"Benjamin"-> start {fire archive, lysis} +22 #"detached from Benjamin"Selecting Cartridge 51 +7 "valve0"D^% +9 "core" %8[ O; +2 "syringe1"C_& +5 "core" &j] +2 "syringe1"CK% +5 "core" %ݠs +2 "valve1"Ea& +4 "core" &gO7  +3 ."detached from Benjamin"Carousel.seek 51 +3 "carousel0"Fb'0 +48 "core" ' +5 "carousel0"F\\% +3 "core" %0?חCw 2 +3 ."detached from Benjamin"Carousel.reconfigure :align + "carousel0"F#` F 22 +9 "syringe1"Cd& +3 "core" &qX +3 ."detached from Benjamin"Lysis1.in :fast +2 "syringe1"C?d P[<2 +8 ."detached from Benjamin"Lysis1.seek :locked +3 "syringe1"Ce( +251 "core" ( +3 "valve0"Dh% +7 "core" %8[O +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +2 "valve0"Dj'Z +8 "syringe1"C`% +7 "core" %ݜ] } +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"CI'6 +68 "core" ' +36 ' +6 ."detached from Benjamin"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +8 FG% +3 "core" %0ZזbD  +6 #"detached from Benjamin"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from Benjamin"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +11 ."detached from Benjamin"Gate.power :cartridge,:OFF +7 #Mounted archive Cartridge +7 "valve0"Dl% +5 "core" %ZG`O- +3 "syringe1"Cg& +4 "core" & Ty +3 "syringe1"Cm% +3 "core" %6΁g ~ +58 "carousel0"Ft% +5 "core" %0זD\\ \  +12 !"detached from nadia#2"o:Cmd::Error: mesg"(Cannot process while in READY state:@timestampu: Time :bt[">/home/esp/esp2/utils/auv/cmd.rb:467:in `startProcessing!'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'""nadia:17:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +8 #"FILTERING"Preparing to filter archiveHiBiomass_bac cartridge in slot 51 +4 #"detached from nadia#2"Waiting to Resume from Checkpoint "Selecting Cartridge 50" +5 "valve0"Ds% +9 "core" %ZGeO0 +2 "syringe1"Cq& +5 "core" &T +2 "syringe1"Cp% +4 "core" %6 q +2 ."FILTERING"Gate.power :sampler,:OFF +40 #Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"Go& +9 "core" & +41 "carousel1"G{& +3 "core" & +47 "carousel1"Gn& +3 "core" & +47 "carousel1"G}& +4 "core" &4 +46 "carousel1"Gi& +3 "core" &Y +47 "carousel1"G=& +3 "core" ǽ&Y +47 "carousel1"G& +3 "core" LJ&S +48 "carousel1"G& +4 "core" DŽ& +46 "carousel1"G\ & +3 "core" NJ&f +47 "carousel1"G& +3 "core" ǁ&kz +47 "carousel1"G& +4 "core" ǂ& +46 "carousel1"G & +4 "core" lj& +46 "carousel1"G]& +3 "core" &] +47 "carousel1"Gc& +3 "core" &o +5 "Can"k^.z2t7O + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 27C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +10 .PhaseSeparator.in :filling +2 "carousel1"G?@@ +7 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +6 "core" Lj, +5 ."FILTERING"Gate.power :sampler,:ON +539 "core" Lj,Ey +7 #"FILTERING"Head pressure reached 3.07psi in 6 seconds +3 "carousel0"F % +7 "core" Ƌ%0זD \  +4 #"FILTERING"Priming sample loop w/20ml +2 .SamplePump.setPosition! 0ml +6 "sampler0"H7 +6 ."FILTERING"Exhaust.close +3 "sampler0"H*0 +152 "core" Ȫ0 +2 ."FILTERING"Bypass.open +2 "sampler1"Ir1 +153 "core" 1 +73 "ambient0"J-% +3 "core" ʭ% +4 "Can"k^.z2s>І +2 "ambient0"J/% +5 "core" ʯ% +2 ."FILTERING"Exhaust.open +2 "sampler0"H~2 +145 "core" 2 +73 ."closeBypass"Bypass.close +8 "sampler1"I/ +153 "core" Ƀ/ +74 "sampler0"H% +3 "core" %`1 +3 "ambient0"Ju% +4 "core" %} +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"Jx+ +6 "core" + +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +8 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G, +6 "core" Ǔ, +3 "ambient0"J % +5 "core" ʠ% +3 "sampler0"H% +6 "core" ȕ%Ta +3 "sampler0"H% +6 "core" Ș%[e +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "16:51:08.33" +4042 "Can"m]. z2s?7 +452 m]. z2s?p* +3 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(q@23 22Q +10 ."FILTERING"SamplePump.seek 20ml,1:50 +4 "sampler0"H'm +54 ."pumpStarted"SamplePump.configure :prime +3 "sampler0"H#d }m#(q@23 22Q +2598 "core" ȗ' +2 "sampler0"H% +5 "core" Ȕ%mn 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +13 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +9 ."FILTERING"Bypass.open +2 "sampler1"I1 +159 "core" ɒ1 +2 ."FILTERING"Intake.close +4 "sampler0"H/ +149 "core" Ȏ/ +2 "carousel0"F% +6 "core" Ə%0חC \  +5 ."FILTERING"Toroid.in :hold +3 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +3 "valve0"Dw( +171 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"D(m +209 "core" Ć( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +9 ."FILTERING"Toroid.seek :clear +3 "valve0"D( +373 "core" ę( +2 "ambient0"J% +4 "core" ʝ%z +4 "Can"m]. z2soI +2 "ambient0"J% +4 "core" ʜ%~ +3 "ambient0"J% +4 "core" ʛ% +3 #"FILTERING"Pressurizing cartridge to 26psi above 14.9psia +3 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +7 ."FILTERING"Intake.open +3 "sampler0"H 1 +144 "core" ȍ1 +3 ."FILTERING"Bypass.close +3 "sampler1"I / +152 "core" Ɍ/ +4 ."FILTERING"Exhaust.close +2 "sampler0"H0 +150 "core" Ȑ0 +3 "sampler0"H% +60 "core" ȟ%Y +3 "ambient0"J'% +4 "core" ʧ%o +3 ."FILTERING"Abs.in :default +5 "ambient0"J>9 e  +9 ."FILTERING"Abs.hold +2 "ambient0"J+ +7 "core" ʞ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G., +9 "core" Ǯ, +3 "sampler0"H!% +6 "core" ȡ%B +2 "sampler0"H1% +5 "core" ȱ%i +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>mu0K3 22Q +9 ."FILTERING"SamplePump.seek 2.3ml,40 seconds +3 "sampler0"H&' +943 "core" Ȧ' n 2 +4 "sampler0"H2% +5 "core" Ȳ%  2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Intake.close +3 "sampler0"H$/ +148 "core" Ȥ/ +2 "ambient0"J%% +6 "core" ʥ%H +2 #"FILTERING"Pressurized cartridge with 0.373ml to 26.2psi +2 #Isolated @38.7psia (23.9psi) +7 #Verifying cartridge holds at least 0.65..0.85 of 38.7psia +8 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"Jk% +7 "core" %M +44 "ambient0"J8% +3 "core" ʸ%" +47 "ambient0"J% +3 "core" ʚ% +47 "ambient0"J0% +4 "core" ʰ% +46 "ambient0"J5% +4 "core" ʵ% +46 "ambient0"J4% +3 "core" ʴ%a +47 "ambient0"J6% +3 "core" ʶ% +47 "ambient0"JT% +3 "core" % +47 "ambient0"J:% +3 "core" ʺ% +47 "ambient0"J;% +3 "core" ʻ% +3 #"FILTERING"Cartridge pressure fell 6% to 36.4psia +2 "carousel0"Ff% +10 "core" %0זC \  +2 #"FILTERING"Sampling 1000.0ml +2 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +12 ."FILTERING"Exhaust.open +3 "sampler0"H#2 +152 "core" ȣ2 +3 ."FILTERING"Intake.open +2 "sampler0"H<1 +149 "core" ȼ1 +5 "Can"m]. z2sjK +3 "ambient0"J>% +4 "core" ʾ%G +4 "ambient0"J7% +4 "core" ʷ%d +3 ."FILTERING"Abs.in :default +3 "ambient0"J>:< e  +7 ."FILTERING"Abs.hold +3 "ambient0"J"+ +5 "core" ʢ+ +4 ."FILTERING"PhaseSeparator.hold + "carousel1"G, +10 "core" Ǒ, +2 "ambient0"J3% +4 "core" ʳ%= +4 "sampler0"HE% +4 "core" %q  +3 "sampler0"HF% +4 "core" %  +7 ."FILTERING"SamplePump.in :start +6 "sampler0"H>^ m#(@2@K3 22Q +8 ."FILTERING"SamplePump.seek 1000ml,1:07:10 +3 "sampler0"HA'g+ +51 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d m#(@2@K3 22Q +227 HB% +3 "core" %\ Q *R 2 +3 "ambient0"JH% +5 "core" % | + #"sampling"Cartridge 51 filtering 2/1000ml at 0.76ml/s with 17.9psi vacuum 3.7m deep (20.2psia) +1001 #"nadia"-> upsync +3607 "Can"m]r.%z3sMW +1386 "sampler0"H9% +3 "core" ȹ%7 5 2 +3 "ambient0"JC% +3 "core" %b1 { +3 #"sampling"Cartridge 51 filtering 47/1000ml at 0.75ml/s with 18.5psi vacuum +5987 "sampler0"HJ% +3 "core" % 3} 2 +3 "ambient0"JN% +4 "core" %L | +2 #"sampling"Cartridge 51 filtering 91/1000ml at 0.74ml/s with 14.8psi vacuum +4563 "Can"j\\./z+sOWT +1426 "sampler0"HO% +4 "core" %k < ~2 +3 "ambient0"J?% +4 "core" ʿ% } +2 #"sampling"Cartridge 51 filtering 135/1000ml at 0.73ml/s with 20.8psi vacuum +5987 "sampler0"HD% +3 "core" %\   2 +3 "ambient0"JM% +3 "core" %p | +3 #"sampling"Cartridge 51 filtering 178/1000ml at 0.72ml/s with 21.1psi vacuum +4524 "Can"k\\.3z1sOV6 +1464 "sampler0"HL% +4 "core" %y 0i 2 +4 "ambient0"JR% +3 "core" % } +3 #"sampling"Cartridge 51 filtering 220/1000ml at 0.70ml/s with 18.0psi vacuum +5985 "sampler0"HS% +3 "core" % 1ؔ{ ~2 +3 "ambient0"JU% +3 "core" %qL  +3 #"sampling"Cartridge 51 filtering 262/1000ml at 0.69ml/s with 14.3psi vacuum +4489 "Can"j[B.;z6sTV΢T +1499 "sampler0"HV% +3 "core" %{ E t 2 +3 "ambient0"JW% +4 "core" %D { +2 #"sampling"Cartridge 51 filtering 303/1000ml at 0.69ml/s with 20.8psi vacuum +5988 "sampler0"HX% +3 "core" %Z\ / 2 +3 "ambient0"JZ% +4 "core" %j\ | +2 #"sampling"Cartridge 51 filtering 344/1000ml at 0.68ml/s with 25.0psi vacuum +4452 "Can"i[.Bz6sQVr +1537 "sampler0"HP% +3 "core" %7 /v( 2 +3 "ambient0"J(% +59 "core" ʨ% } +2 #"sampling"Cartridge 51 filtering 384/1000ml at 0.67ml/s with 20.3psi vacuum 4.0m deep (20.6psia) +5933 "sampler0"H% +3 "core" ȅ% `  2 +3 "ambient0"J,% +4 "core" ʬ%L | +2 #"sampling"Cartridge 51 filtering 423/1000ml at 0.65ml/s with 22.5psi vacuum +4417 "Can"i[B.Kz/sMWF +1571 "sampler0"H|% +3 "core" % ߴ $ ~2 +3 "ambient0"J^% +4 "core" % ~ +2 #"sampling"Cartridge 51 filtering 461/1000ml at 0.64ml/s with 32.8psi vacuum +1144 #"Benjamin#2"Session Begun +1066 #-> can +9 "Can"i[B.Kz/s?U+ +1302 #"Benjamin#2"-> can! +4 #Polling Can every 2:00 +57 "Can"j[.Oz8sOUS +2405 "sampler0"H_% +4 "core" %\ S "] 2 +3 "ambient0"JK% +3 "core" %?( ~ +2 #"sampling"Cartridge 51 filtering 499/1000ml at 0.63ml/s with 18.0psi vacuum 3.8m deep (20.3psia) +4384 "Can"j[.Pz4sOV +1275 #"Benjamin#2"-> H.psi +7 !o:NameError : mesg"uninitialized constant H:@timestampu: Time Шx: name:H:bt["&Benjamin#2:3:in `topLevelBinding'" Head.psi +3 "carousel1"Gd& +9 "core" &lA +4311 #"Benjamin#2"-> Head.psi +3 "carousel1"G@& +12 "core" &C| +1094 "sampler0"Hh% +3 "core" % A |' 2 +3 "ambient0"J`% +4 "core" %z +2 #"sampling"Cartridge 51 filtering 573/1000ml at 0.61ml/s with 24.8psi vacuum +2867 #"Benjamin#2"-> Head.counts +5 "carousel1"GQ& +6 "core" &E +1469 "Can"jZ.Rz8sWT! +1641 "sampler0"HG% +3 "core" % Z\ \  2 +3 "ambient0"J[% +4 "core" %y8  +2 #"sampling"Cartridge 51 filtering 609/1000ml at 0.60ml/s with 29.9psi vacuum +5989 "sampler0"HY% +4 "core" % Nڠ 2 +4 "ambient0"Jl% +4 "core" %;  +2 #"sampling"Cartridge 51 filtering 644/1000ml at 0.59ml/s with 29.2psi vacuum +4307 "Can"lY.Xz4sTU2 +1679 "sampler0"Hg% +4 "core" %* 6 2 +4 "ambient0"Jm% +4 "core" %9 } +2 #"sampling"Cartridge 51 filtering 678/1000ml at 0.57ml/s with 13.2psi vacuum 9.4m deep (28.6psia) +2762 "core" 'X 2 +4 "sampler0"Ht% +4 "core" %鎲 2 +2 ."FILTERING"SamplePump.stop +2 "sampler0"H5 +12 ."closeIntake"Intake.close +5 "sampler0"Hs/ +8 ."closeExhaust"Exhaust.close +8 "carousel0"Fq% +6 "sampler0"Hp0 +13 "core" %0חD \  +7 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +11 ."FILTERING"Toroid.seek :bypass +7 "valve0"Do( +87 "core" / +27 0 +239 ( +5 ."FILTERING"Toroid.in :default +3 "valve0"D? ֜2 +11 ."FILTERING"Toroid.seek :clear +3 "valve0"D{( +357 "core" ( +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +11 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +13 "carousel0"Fn% +3 "core" %0חER \  +6 "carousel0"F}% +5 "core" %0חC? \  +6 "carousel0"Fi% +4 "core" %0זD \  +8 !"FILTERING"o:Cartridge::Sampler::Leaky : mesg"NSerious Leak or Stall -- 0.45ml/s made only -62.3psi vacuum after 693.9ml:@timestampu: Time g[':@checkpoint: omitted:bt["=/home/esp/esp2/utils/cartridge/sampler.rb:522:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"E/home/esp/esp2/utils/cartridge/sampler.rb:391:in `sampleQuietly'"7/home/esp/esp2/utils/gensampler.rb:223:in `sample'">/home/esp/esp2/utils/cartridge/sampler.rb:427:in `filter'"A/home/esp/esp2/type/auv/preconfig.rb:86:in `filterCartridge'"=/home/esp/esp2/utils/auv/cmd.rb:378:in `startFiltering!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"=/home/esp/esp2/utils/auv/cmd.rb:349:in `startFiltering!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 693.86ml" +285 ."sourcePumpShutdown"Gate.power :sampler,:OFF +3594 #"nadia"-> Sampler.psi +3 "sampler0"H=% +8 "core" Ƚ% +2729 "Can"i[6.Yz4tQ#, +3349 #"Benjamin#2"-> Head.psi +3 "carousel1"G& +8 "core" LJ& +995 #"Benjamin#2"-> Head.count +3015 #-> Head.counts +2 "carousel1"G& +7 "core" DŽ&x +755 #"Benjamin#2"-> Head.psi +3 "carousel1"G\ & +8 "core" NJ&| +787 #"Benjamin#2"-> Head.counts +3 "carousel1"G& +8 "core" ǁ&nv +2149 #"nadia"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +868 "Can"lZ.Sz5s@i\ 8& +6009 #"Benjamin#2"-> Head.counts +3 "carousel1"G& +8 "core" ǂ& +1658 #"Benjamin#2"-> Head.psi +3 "carousel1"G & +8 "core" lj&x +1917 #"Benjamin#2"-> Head.counts +6 "carousel1"G]& +5 "core" &%ac +956 #"Benjamin#2"-> Head.psi +3 "carousel1"Gc& +8 "core" &a +1379 "Can"lZ.Sz3s=D +1647 #"Benjamin#2"-> Head.counts +3 "carousel1"G & +9 "core" Nj&D +2586 #"Benjamin#2"-> Head.counts +8 "carousel1"G*& +4 "core" Ǫ&" +6324 #"nadia"-> Power.sampler :OFF +4 .Gate.power :sampler,:OFF +1379 "Can"l[5.Yz7tOpb +1402 #"nadia"-> Power.sampler :ON +2 .Gate.power :sampler,:ON +7878 #-> Sampler.refill +3 .Bypass.open +7 "sampler1"Ir1 +155 "core" 1 +2 ."nadia"Intake.open +3 "sampler0"H-1 +148 "core" ȭ1 +3 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +10 H/% +3 "core" ȯ%/i +3 ."nadia"SamplePump.in :default +2 "sampler0"H>^ #(22 +9 ."nadia"SamplePump.seek 30ml +2 "sampler0"H~'` +2330 "Can"kZ.[z3sPV +302 ."nadia"SamplePump.hold +75 "sampler0"H+ +6 "core" 'o 1 2 +4 ȃ+ +3 ."nadia"delay 1 second +103 ."closeIntake"Intake.close +4 "sampler0"H/ +4 ."closeBypass"Bypass.close +15 "carousel0"Fu% +6 "sampler1"I / +5 "core" %0טmJ \ +117 / +34 ɠ/ +4 !"nadia"o:UserAbort : mesg"+Sample Pump (SP) seek to 30ml Aborted!:@timestampu: Time EMRL:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:125:in `refill'""nadia:30:in `topLevelBinding'" BACsampler.prime 50,nil +3 "carousel0"F% +13 "core" ƕ%0טK \ +4 #"nadia"Priming sample loop w/50ml +2 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +10 ."nadia"Bypass.open +6 "sampler1"I1 +151 "core" ɘ1 +73 "ambient0"J% +3 "core" ʈ% +5 "Can"kZ.[z3s> !4 + "ambient0"Jv% +5 "core" %\ +3 ."nadia"Exhaust.open +2 "sampler0"H2 +151 "core" Ȕ2 +73 ."closeBypass"Bypass.close +7 ."nadia"Intake.open +8 "sampler1"I/ +5 "sampler0"Hx1 +146 "core" 1 + ɒ/ +74 "sampler0"H% +3 "core" ȓ%L +3 "ambient0"J% +3 "core" ʎ% +3 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +10 ."nadia"Abs.hold +2 "ambient0"J+ +8 "core" ʏ+ +3 ."nadia"PhaseSeparator.hold + "carousel1"G+, +10 "core" ǫ, +3 "ambient0"Jw% +5 "core" % +5 "sampler0"H% +5 "core" Ȇ%O +3 "sampler0"H% +4 "core" ȝ%o +5 ."nadia"SamplePump.in :start +4 "sampler0"H>^ }m#(q@23 22Q +10 ."nadia"SamplePump.seek 50ml,3:50 +7 "sampler0"H' +48 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(q@23 22Q +3620 "core" Ȝ' 64 2 +4 "sampler0"H% +4 "core" ț%h 2 +2 ."nadia"SamplePump.stop +2 "sampler0"H5 +11 ."closeIntake"Intake.close +6 ."closeExhaust"Exhaust.close +7 "sampler0"H / +8 H 0 +3 "carousel0"F% +11 "core" Ɛ%0חI \  +127 ȍ/ +16 Ȍ0 +2 ."nadia"Abs.stop +2 "ambient0"J5 +9 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +9 !"nadia"o:Cartridge::Sampler::Leaky : mesg"NSerious Leak or Stall -- 0.76ml/s made only -107.6psi vacuum after 27.8ml:@timestampu: Time |SP:@checkpoint: omitted:bt["=/home/esp/esp2/utils/cartridge/sampler.rb:522:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'""nadia:31:in `topLevelBinding'" Sampler.psi +4 "sampler0"H% +9 "core" ȟ%m +1267 #"nadia"-> Leak.psia +4 "ambient0"J'% +8 "core" ʧ% +1426 "Can"jZ.Xz4s=( +1436 #"Benjamin"Session Ended +7 #"Benjamin#2"Session Ended +8 !"Benjamin#2's_reader"o:Errno::EPIPE:\ errnoi%: mesg"Broken pipe:bt["3/home/esp/esp2/lib/cmdserver.rb:376:in `write'"3/home/esp/esp2/lib/cmdserver.rb:376:in `print'"4/home/esp/esp2/lib/cmdserver.rb:376:in `output'"1/home/esp/esp2/lib/cmdserver.rb:490:in `run'"2/home/esp/esp2/lib/cmdserver.rb:486:in `loop'"1/home/esp/esp2/lib/cmdserver.rb:486:in `run'"1/home/esp/esp2/lib/cmdserver.rb:543:in `run'"1/home/esp/esp2/lib/thread.rb:892:in `create'"0/home/esp/esp2/lib/thread.rb:736:in `start'"1/home/esp/esp2/lib/thread.rb:889:in `create'"5/home/esp/esp2/lib/thread.rb:888:in `initialize'"%/home/esp/esp2/lib/thread.rb:888 +5668 #"na"Session Begun +2074 #-> loop{puts Sampler.psi;Delay.sleep 0.5} +9 "sampler0"H!% +4 "core" ȡ% +54 "sampler0"H1% +3 "core" ȱ% +53 "sampler0"H% +3 "core" ȗ%x +54 "sampler0"H2% +3 "core" Ȳ%; +54 "sampler0"H$% +3 "core" Ȥ%p +53 "sampler0"H%% +3 "core" ȥ%l +54 "sampler0"Hk% +3 "core" %d +54 "sampler0"H8% +3 "core" ȸ%b +54 "sampler0"H% +3 "core" Ț% +53 "sampler0"H% +3 "core" Ȟ%W +54 "sampler0"H.% +3 "core" Ȯ% +54 "sampler0"H0% +3 "core" Ȱ% +53 "sampler0"H5% +3 "core" ȵ%l +54 "sampler0"H4% +3 "core" ȴ% +56 "sampler0"H6% +4 "core" ȶ% +53 "sampler0"HT% +3 "core" % +54 "sampler0"H:% +4 "core" Ⱥ%| +53 "sampler0"H;% +4 "core" Ȼ%~ +53 "sampler0"Hf% +4 "core" %t +54 "sampler0"H#% +3 "core" ȣ%j +53 "sampler0"H<% +3 "core" ȼ%i +55 "sampler0"H>% +5 "core" Ⱦ%a +55 "sampler0"H7% +4 "core" ȷ% +53 "sampler0"H3% +5 "core" ȳ% +54 "sampler0"HE% +3 "core" % +53 "sampler0"HF% +3 "core" % +33 #"nadia"-> Sampler.tare +6 !o:Cartridge::Sampler::Error: mesg"#Cannot tare with Intake closed:@timestampu: Time Z:bt["8/home/esp/esp2/utils/cartridge/deep.rb:97:in `tare'""nadia:34:in `topLevelBinding'" Sampler.tare! +2 .Bypass.open +6 "sampler1"IL1 +23 "sampler0"HR% +4 "core" %ŋ +54 "sampler0"HS% +3 "core" %‹ +54 "sampler0"HU% +3 "core" % +12 1 +41 "sampler0"HV% +5 "core" %) +7 ."nadia"Intake.open +2 "sampler0"HW1 +16 !"nadia"o:UserAbort: mesg"deferring User Abort!!:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'"*nadia aborter-:1:in `topLevelBinding'" Power.sampler :OFF +3 .Gate.power :sampler,:OFF +38 "sampler0"Hl% +3 "core" % +53 "sampler0"Hg% +3 "core" % +54 "sampler0"Hm% +3 "core" % +54 "sampler0"Ht% +5 "core" % +53 "sampler0"Hq% +3 "core" % +54 "sampler0"Hs% +3 "core" %v +54 "sampler0"Hp% +3 "core" %m +54 "sampler0"HA% +3 "core" %r +54 "sampler0"H% +3 "core" ș%l +53 "sampler0"Ho% +3 "core" % +54 "sampler0"Hn% +3 "core" % +53 "sampler0"H}% +3 "core" % +53 "sampler0"Hi% +5 "core" % +55 "sampler0"H"% +4 "core" Ȣ% +34 #"nadia"-> Sampler.tare! +4 .Bypass.open +3 "sampler1"I1 +13 "sampler0"H=% +3 "core" Ƚ% +53 "sampler0"H% +4 "core" ȇ%މ +53 "sampler0"H% +3 "core" Ȅ%ډ +24 ɑ1 +30 "sampler0"H\ % +3 "core" Ȋ% +22 ."nadia"Intake.open +2 "sampler0"H1 +29 H% +3 "core" Ȃ%! +53 "sampler0"H % +3 "core" ȉ% +55 "sampler0"H]% +6 "core" % +5 ȁ1 +2 "ambient0"Jc% +4 "core" % +4 #"nadia"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi + "sampler0"H % +9 "core" ȋ%a +29 "sampler0"H*% +4 "core" Ȫ%c +9 "sampler0"Hr% +3 "core" %f +41 "sampler0"H-% +3 "core" ȭ%X +7 "sampler0"H/% +3 "core" ȯ%[ +43 "sampler0"Hu% +3 "core" %T +4 "sampler0"H~% +5 "core" %P +38 "sampler0"H% +3 "core" %K +12 "sampler0"H % +3 "core" Ƞ%M +32 "sampler0"H% +3 "core" ȕ%K +5 "Can"jZ.\\z5t7/V +2 #"nadia"Vacuum tare is 54% of full scale at 27C (drifted 2%) +3 "ambient0"J% +7 "core" ʘ% +3 ."closeEx"Bypass.close +2 "sampler1"I/ +6 ."nadia"Intake.close +4 "sampler0"Hv/ +10 H% +3 "core" Ȕ%݉ +54 "sampler0"H% +3 "core" Ȓ% +54 "sampler0"Hx% +3 "core" % +16 Ɉ/ +5 / +33 "sampler0"H% +3 "core" ȓ%| +54 "sampler0"H% +4 "core" Ȏ% +113 "sampler0"Hw% +3 "core" %wk +54 "sampler0"H% +3 "core" Ȇ%p +54 "sampler0"H% +3 "core" ȝ%i +53 "sampler0"H% +3 "core" ȃ%ku +54 "sampler0"H% +4 "core" ț%_ +54 "sampler0"H% +3 "core" Ȑ%W +53 "sampler0"H % +3 "core" ȍ% +55 "sampler0"H % +3 "core" Ȍ% +53 "sampler0"H% +3 "core" Ȝ% +54 "sampler0"H% +5 "core" ȏ% +53 "sampler0"H+% +4 "core" ȫ% +54 "sampler0"H% +3 "core" ȟ%z +53 "sampler0"H'% +3 "core" ȧ%Z +54 "sampler0"H!% +3 "core" ȡ%z +53 "sampler0"H1% +3 "core" ȱ% +54 "sampler0"H% +3 "core" ȗ%a~ +53 "sampler0"H2% +3 "core" Ȳ%wz +54 "sampler0"H$% +5 "core" Ȥ%o +54 "sampler0"H%% +4 "core" ȥ%mz +55 "sampler0"Hk% +3 "core" %gr +54 "sampler0"H8% +3 "core" ȸ%]n +54 "sampler0"H% +3 "core" Ț%j +6 #"nadia"-> Power.sampler :ON +12 .Gate.power :sampler,:ON +36 "sampler0"H% +3 "core" Ȟ% +54 "sampler0"H.% +3 "core" Ȯ% +53 "sampler0"H0% +3 "core" Ȱ%_ +53 "sampler0"H5% +3 "core" ȵ% +54 "sampler0"H4% +3 "core" ȴ% +54 "sampler0"H6% +4 "core" ȶ% +54 "sampler0"HT% +3 "core" %s +54 "sampler0"H:% +3 "core" Ⱥ%h +54 "sampler0"H;% +3 "core" Ȼ%b +53 "sampler0"Hf% +4 "core" %` +55 "sampler0"H#% +3 "core" ȣ%W +54 "sampler0"H<% +3 "core" ȼ% +54 "sampler0"H>% +3 "core" Ⱦ% +54 "sampler0"H7% +4 "core" ȷ% +54 "sampler0"H3% +3 "core" ȳ% +53 "sampler0"HE% +3 "core" % +54 "sampler0"HF% +3 "core" % +54 "sampler0"HB% +3 "core" % +54 "sampler0"HH% +3 "core" % +54 "sampler0"H&% +3 "core" Ȧ% +54 "sampler0"H9% +3 "core" ȹ%w +54 "sampler0"HC% +3 "core" %y +54 "sampler0"HJ% +4 "core" %p +53 "sampler0"HN% +4 "core" % +34 #"nadia"-> 2.times{Sampler.refill} +3 .Bypass.open +5 "sampler1"IO1 +11 "sampler0"H?% +3 "core" ȿ% +55 "sampler0"HD% +3 "core" % +54 "sampler0"HM% +3 "core" % +26 1 +2 ."nadia"Intake.open +2 "sampler0"HR1 +24 HS% +4 "core" %7 +53 "sampler0"HU% +3 "core" % +54 "sampler0"HL% +3 "core" %# +6 1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +7 HV% +3 "core" %D +7 ."nadia"SamplePump.in :default +3 "sampler0"H>^ #(22 +8 ."nadia"SamplePump.seek 30ml +3 "sampler0"HX'` +12 HZ% +3 "core" %uE 2 +53 "sampler0"HW% +3 "core" % $= 2 +54 "sampler0"H% +3 "core" ȅ% " 2 +55 "sampler0"H,% +5 "core" Ȭ% # 2 +54 "sampler0"H|% +3 "core" %  2 +53 "sampler0"H(% +3 "core" Ȩ% H ) 2 +54 "sampler0"HP% +3 "core" % i 2 +53 "sampler0"H^% +3 "core" % $r 2 +54 "sampler0"H_% +3 "core" %\\ 'ލ 2 +53 "sampler0"HK% +3 "core" % !ӊ 2 +55 "sampler0"Ha% +6 "core" %- %Ѝ 2 +54 "sampler0"H\\% +4 "core" % 1̌ 2 +54 "sampler0"Hd% +3 "core" % 'w 2 +54 "sampler0"H@% +3 "core" %q ) 2 +54 "sampler0"Hh% +3 "core" % & ~2 +55 "sampler0"H`% +3 "core" %#; *- 2 +53 "sampler0"HQ% +3 "core" %% ( 2 +54 "sampler0"HG% +3 "core" %' &v 2 +54 "sampler0"H[% +3 "core" %*T &^ 2 +53 "sampler0"HY% +3 "core" %, & 2 +54 "sampler0"Hl% +5 "core" %/ ,Is 2 +53 "sampler0"Hg% +3 "core" %1{ +W 2 +54 "sampler0"Hm% +3 "core" %3 ( 2 +54 "sampler0"Ht% +3 "core" %6A "u 2 +113 "sampler0"Hq% +3 "core" %; $W 2 +54 "sampler0"Hs% +3 "core" %={ &C ~2 +54 "sampler0"Hp% +3 "core" %? 1 2 +54 "sampler0"HA% +3 "core" %B< / 2 +53 "sampler0"H% +4 "core" ș%D , 2 +54 "sampler0"Ho% +3 "core" %F 0 ~2 +54 "sampler0"Hn% +4 "core" %Ig 1 2 +54 "sampler0"H}% +3 "core" %K * 2 +53 "sampler0"Hi% +3 "core" %N% -}c 2 +54 "sampler0"H"% +3 "core" Ȣ%P .| 2 +54 "sampler0"H=% +3 "core" Ƚ%R -ݍi 2 +54 "sampler0"H% +4 "core" ȇ%UD *ٌ; 2 +54 "sampler0"H% +3 "core" Ȅ%W - ~2 +54 "sampler0"H% +4 "core" ȑ%Z )Ώ 2 +54 "sampler0"H\ % +3 "core" Ȋ%\\ -ȋ 2 +54 "sampler0"H% +3 "core" Ȃ%^ , 2 +53 "sampler0"H % +3 "core" ȉ%a< +A 2 +54 "sampler0"H]% +3 "core" %c , 2 +54 "sampler0"H% +3 "core" ȁ%e *K 2 +53 "sampler0"Hc% +3 "core" %hU ) 2 +55 "sampler0"H % +4 "core" ȋ%j ' 2 +54 "sampler0"H*% +3 "core" Ȫ%m* ,Z 2 +53 "sampler0"Hr% +3 "core" %o / 2 +54 "sampler0"H-% +3 "core" ȭ%q 3l 2 +53 "sampler0"H/% +3 "core" ȯ%t@ ,| 2 +54 "sampler0"Hu% +3 "core" %v /Î 2 +53 "sampler0"H~% +3 "core" %x /n 2 +55 "sampler0"H% +5 "core" %{i 1 ~2 +54 "sampler0"H % +3 "core" Ƞ%} 3 2 +53 "sampler0"H% +3 "core" ȕ%- )` 2 +54 "sampler0"H% +3 "core" Ș% ) 2 +54 "sampler0"H% +4 "core" Ȕ% 1? ~2 +53 "sampler0"H% +3 "core" Ȓ%S + 2 +54 "sampler0"Hx% +3 "core" % ,N 2 +56 "sampler0"H% +4 "core" Ȉ%2 /~7 2 +53 "sampler0"Hv% +4 "core" % *w 2 +53 "sampler0"H% +3 "core" ȓ% .ߍ6 2 +54 "sampler0"H% +3 "core" Ȏ%M (֊ 2 +53 "sampler0"Hw% +3 "core" % *֎ 2 +54 "sampler0"H% +3 "core" Ȇ% )Ɋ 2 +53 "sampler0"H% +4 "core" ȝ%^ 2ʎ" 2 +54 "sampler0"H% +6 "core" ȃ% ) ~2 +53 "sampler0"H% +3 "core" ț%C 2 2 +54 "sampler0"H% +3 "core" Ȑ% 1 2 +53 "sampler0"H % +3 "core" ȍ% W 2 +34 ' +2 ."nadia"Bypass.close +7 "sampler1"I / +11 "sampler0"H% +3 "core" Ȝ%`P +53 "sampler0"H% +3 "core" ȏ%`L +54 "sampler0"H+% +3 "core" ȫ%`N +27 Ɍ/ +2 ."nadia"Exhaust.open +4 "sampler0"H2 +20 H'% +6 "core" ȧ%`Z +55 "sampler0"H!% +4 "core" ȡ%`p +53 "sampler0"H1% +3 "core" ȱ%`> +12 ȟ2 +2 "sampler0"H% +5 "core" ȗ%`, +3 ."nadia"SamplePump.seek 55ml +3 "sampler0"H2'-[ +29 H$% +3 "core" Ȥ% 2 +54 "sampler0"H%% +3 "core" ȥ% +ܐ 2 +54 "sampler0"Hk% +3 "core" % +Ќ 2 +53 "sampler0"H8% +3 "core" ȸ%x *ΐ 2 +55 "sampler0"H% +5 "core" Ț% (Ì 2 +54 "sampler0"H% +3 "core" Ȟ%Y * 2 +53 "sampler0"H.% +3 "core" Ȯ% * ~2 +54 "sampler0"H0% +4 "core" Ȱ% . 2 +53 "sampler0"H5% +4 "core" ȵ%x 05 2 +53 "sampler0"H4% +3 "core" ȴ% 2' }2 +54 "sampler0"H6% +3 "core" ȶ%4 39 ~2 +55 "sampler0"HT% +4 "core" % . 2 +54 "sampler0"H:% +3 "core" Ⱥ% ,ˎr 2 +55 "sampler0"H;% +3 "core" Ȼ%x .L 2 +54 "sampler0"Hf% +3 "core" % )䓟 ~2 +53 "sampler0"H#% +4 "core" ȣ%7 /s ~2 +53 "sampler0"H<% +4 "core" ȼ%ʢ 3Փ ~2 +54 "sampler0"H>% +3 "core" Ⱦ% (Ԍx 2 +54 "sampler0"H7% +3 "core" ȷ%e 3ʐd 2 +54 "sampler0"H3% +3 "core" ȳ% ) }2 +53 "sampler0"HE% +3 "core" % * 2 +54 "sampler0"HF% +3 "core" %ր , 2 +54 "sampler0"HB% +3 "core" % .j 2 +53 "sampler0"HH% +3 "core" %5 # ~2 +54 "sampler0"H&% +3 "core" Ȧ%ݖ 2 2 +56 "sampler0"H9% +4 "core" ȹ% / 2 +54 "sampler0"HC% +3 "core" %t 72 2 +53 "sampler0"HJ% +3 "core" % 4+ 2 +110 "sampler0"HN% +3 "core" % 0" 2 +53 "sampler0"H?% +3 "core" ȿ% 9 ~2 +54 "sampler0"HD% +3 "core" %< -ۏ= 2 +55 "sampler0"HM% +5 "core" % 0ۑc }2 +54 "sampler0"HO% +3 "core" % +Ŏw 2 +53 "sampler0"HS% +3 "core" %x 1E 2 +54 "sampler0"HU% +3 "core" % 7; 2 +54 "sampler0"HL% +3 "core" %7 . ~2 +53 "sampler0"HR% +3 "core" % 4 2 +40 "Can"jZ~.`z3sSV=6 +14 "sampler0"HV% +3 "core" % / 2 +53 "sampler0"HZ% +7 "core" %a 0d 2 +54 "sampler0"HW% +3 "core" % . 2 +53 "sampler0"H% +3 "core" ȅ%, 2 2 +54 "sampler0"H,% +3 "core" Ȭ% . 2 +53 "sampler0"H|% +5 "core" %\ -ȏ 2 +53 "sampler0"H(% +3 "core" Ȩ% O /_ 2 +53 "sampler0"HP% +3 "core" % 1 2 +55 "sampler0"H^% +6 "core" % 8- 2 +53 "sampler0"H_% +3 "core" % 9 ~2 +54 "sampler0"HK% +3 "core" % 2 2 +53 "sampler0"Ha% +3 "core" %B = 2 +54 "sampler0"H\\% +3 "core" % 6 ~2 +54 "sampler0"Hd% +3 "core" % )Y ~2 +54 "sampler0"H@% +15 "core" % l 0 2 +54 "sampler0"Hh% +3 "core" %#B 3܏, 2 +53 "sampler0"H`% +3 "core" %% 4ݔ0 2 +54 "sampler0"HQ% +3 "core" %' 5؏ 2 +54 "sampler0"HG% +3 "core" %*a 2͓ ~2 +54 "sampler0"H[% +3 "core" %,\ ÍP ~2 +39 Ȳ' +2 ."nadia"delay 1 second +12 "sampler0"HY% +3 "core" %-[ +54 "sampler0"Hl% +3 "core" %-[u; +31 ."closeIntake"Intake.close +5 ."closeExhaust"Exhaust.close +10 "sampler0"Hg/ +6 "carousel0"Fm% +4 "sampler0"Ht0 +7 "core" %0טIy \ +6 "sampler0"Hq% +6 "core" %-[G +53 "sampler0"Hs% +4 "core" %-[ډZ +54 "sampler0"Hp% +4 "core" %-[ىZ +7 / +22 0 +2 ."nadia"Bypass.open +3 "sampler1"IA1 +22 "sampler0"H% +6 "core" ș%-[N +54 "sampler0"Ho% +3 "core" %-[P +54 "sampler0"Hn% +3 "core" %-[T +10 1 +2 ."nadia"Intake.open +3 "sampler0"H}1 +39 Hi% +3 "core" %-[o +54 "sampler0"H"% +3 "core" Ȣ%-[>g +50 1 +2 ."nadia"SamplePump.setPosition! 0ml +2 "sampler0"H7 +8 H=% +3 "core" Ƚ%Kb +3 ."nadia"SamplePump.seek 30ml +3 "sampler0"H'` +7 H% +3 "core" Ȅ%z 2 +54 "sampler0"H% +3 "core" ȑ%x   2 +53 "sampler0"H\ % +3 "core" Ȋ% ) 2 +54 "sampler0"H% +3 "core" Ȃ%/ (R 2 +53 "sampler0"H % +3 "core" ȉ% % 2 +54 "sampler0"H]% +6 "core" %\ /w 2 +55 "sampler0"H% +3 "core" ȁ% q - 2 +53 "sampler0"Hc% +3 "core" % 4g 2 +42 ."nadia"SamplePump.hold +6 "sampler0"H + +8 "core" ȇ'# .䌙 2 +3 "sampler0"H*% +6 "core" ȋ+ + Ȫ%.h 2 +7 ."nadia"delay 1 second +49 "sampler0"Hr% +3 "core" %.I +52 ."closeIntake"Intake.close +5 ."closeBypass"Bypass.close +7 "sampler0"H-/ +6 "carousel0"F/% +3 "sampler1"Iu/ +10 "core" Ư%0חJ \  +6 "sampler0"H~% +5 "core" %.$i +53 "sampler0"H% +3 "core" %.k +54 "sampler0"H % +3 "core" Ƞ%.O +6 ȭ/ +12 / +2 !"nadia"o:UserAbort : mesg"+Sample Pump (SP) seek to 30ml Aborted!:@timestampu: Time me:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:125:in `refill'""nadia:39:in `topLevelBinding'"nadia:39:in `times'""nadia:39:in `topLevelBinding'" BACsampler.prime 50,nil +6 "sampler0"H'% +5 "carousel0"F!% +5 "core" ȧ%. +8 ơ%0טJ_ \ +2 #"nadia"Priming sample loop w/50ml +2 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +7 ."nadia"Bypass.open +2 "sampler1"I11 +28 "sampler0"H% +3 "core" ȟ% +54 "sampler0"H% +3 "core" ȗ%ۊ +54 "sampler0"H$% +4 "core" Ȥ%֊ +6 ɱ1 +47 "sampler0"H%% +4 "core" ȥ% +22 "ambient0"Jk% +3 "core" % +4 "Can"jZ~.`z3s=$99 +2 "ambient0"J8% +5 "core" ʸ% +6 ."nadia"Exhaust.open +2 "sampler0"H2 +10 HX% +3 "core" % +54 "sampler0"H% +3 "core" Ȟ%9 +54 "sampler0"H.% +3 "core" Ȯ%8 +26 Ț2 +28 "sampler0"H0% +3 "core" Ȱ%b8 +43 ."closeBypass"Bypass.close +3 "sampler1"I5/ +6 ."nadia"Intake.open +6 "sampler0"H41 +8 H6% +3 "core" ȶ% +53 "sampler0"HT% +3 "core" %҉ +54 "sampler0"H:% +3 "core" Ⱥ% m +16 ɵ/ +3 ȴ1 +34 "sampler0"H;% +4 "core" Ȼ% +35 "sampler0"Hf% +3 "core" %z +3 "ambient0"J#% +4 "core" ʣ% +3 ."nadia"Abs.in :default +2 "ambient0"J>9 e  +9 ."nadia"Abs.hold +4 "sampler0"H<% +4 "ambient0"J>+ +5 "core" ȼ%}} +7 ʾ+ +2 ."nadia"PhaseSeparator.hold +2 "carousel1"G7, +9 "core" Ƿ, +2 "ambient0"J3% +5 "core" ʳ% +2 "sampler0"HE% +5 "core" %u +2 "sampler0"HF% +5 "core" %x +6 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }n@#(@y3 22Q +9 ."nadia"SamplePump.seek 50ml,3:50 +3 "sampler0"HB' +7 HH% +4 "core" %Ev 2 +44 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n@#(@y3 22Q +8 H&% +3 "core" Ȧ% /bP 2 +54 "sampler0"H9% +3 "core" ȹ% * 2 +53 "sampler0"HC% +3 "core" %@ + 2 +54 "sampler0"HJ% +6 "core" % -G 2 +54 "sampler0"HN% +3 "core" % # . 2 +54 "sampler0"H?% +3 "core" ȿ% -В 2 +53 "sampler0"HD% +4 "core" % .ŕ 2 +54 "sampler0"HM% +3 "core" %D ([ 2 +54 "sampler0"HO% +3 "core" % ) 2 +54 "sampler0"HS% +4 "core" % ) 2 +55 "sampler0"HU% +4 "core" % % 2 +55 "sampler0"HL% +2 "core" % . 2 +54 "sampler0"HR% +3 "core" %N .Ɣg 2 +54 "sampler0"HV% +3 "core" % 3 2 +54 "sampler0"HZ% +3 "core" %# / 2 +54 "sampler0"HW% +3 "core" %%n + 2 +54 "sampler0"H% +3 "core" ȅ%' 4ޑ 2 +55 "sampler0"H,% +3 "core" Ȭ%*? -، 2 +54 "sampler0"H|% +3 "core" %, *Ǒ 2 +54 "sampler0"H(% +3 "core" Ȩ%/ -ː 2 +54 "sampler0"HP% +3 "core" %1b / 2 +53 "sampler0"H^% +3 "core" %3 ( 2 +54 "sampler0"H_% +3 "core" %6 0 2 +54 "sampler0"HK% +3 "core" %8{ / 2 +55 "sampler0"Ha% +5 "core" %: *! 2 +54 "sampler0"H\\% +3 "core" %=_ . 2 +53 "sampler0"Hd% +3 "core" %? &0 2 +54 "sampler0"H@% +3 "core" %B /7 2 +53 "sampler0"Hh% +3 "core" %Dq )A 2 +54 "sampler0"H`% +3 "core" %F (̏ 2 +54 "sampler0"HQ% +3 "core" %I3 ,Ǎ 2 +55 "sampler0"HG% +4 "core" %K 2A ~2 +54 "sampler0"H[% +3 "core" %N 4P 2 +54 "sampler0"HY% +3 "core" %Pk 5 2 +109 "sampler0"Hl% +3 "core" %U 2 2 +54 "sampler0"Hm% +3 "core" %W| 3 ~2 +54 "sampler0"Hq% +4 "core" %Y 2 ~2 +54 "sampler0"Hs% +7 "core" %\\Y .Ґ 2 +53 "sampler0"Hp% +3 "core" %^ 1Β[ 2 +53 "sampler0"Hg% +3 "core" %a" /Ēf 2 +54 "sampler0"Ht% +3 "core" %c + 2 +53 "sampler0"H% +4 "core" ș%e 1 ~2 +54 "sampler0"Ho% +3 "core" %hG , 2 +54 "sampler0"Hn% +3 "core" %j . 2 +55 "sampler0"HA% +4 "core" %m , 2 +53 "sampler0"Hi% +3 "core" %ov ,D 2 +54 "sampler0"H"% +3 "core" Ȣ%q 2 2 +53 "sampler0"H}% +3 "core" %t- 2) 2 +54 "sampler0"H=% +3 "core" Ƚ%v 0掹 2 +54 "sampler0"H% +3 "core" Ȅ%x 3 2 +54 "sampler0"H% +4 "core" ȑ%{\\ 4׏, 2 +55 "sampler0"H\ % +3 "core" Ȋ%} /ɒQ 2 +54 "sampler0"H% +3 "core" Ȃ%( 7ŏ 2 +54 "sampler0"H % +3 "core" ȉ% 1 2 +54 "sampler0"H]% +3 "core" % - ~2 +54 "sampler0"H2% +3 "core" Ȳ%M 7( 2 +54 "sampler0"H% +3 "core" ȁ% / 2 +53 "sampler0"Hc% +3 "core" % + 2 +55 "sampler0"H*% +4 "core" Ȫ%} 1 ~2 +53 "sampler0"Hr% +4 "core" % . 2 +54 "sampler0"H/% +3 "core" ȯ%A , 2 +54 "sampler0"H~% +3 "core" % *ӌL ~2 +54 "sampler0"H% +3 "core" % /“~ ~2 +53 "sampler0"H % +3 "core" Ƞ%] -ɌN 2 +54 "sampler0"H-% +3 "core" ȭ% 0 2 +55 "sampler0"H% +5 "core" ȇ%2 5 2 +55 "sampler0"Hu% +3 "core" % 8 }2 +53 "sampler0"H% +3 "core" ȕ% 3 2 +54 "sampler0"H% +4 "core" Ș%k 9 2 +53 "sampler0"H% +3 "core" Ȕ% 3 ~2 +54 "sampler0"H% +3 "core" Ȓ%& 3k 2 +53 "sampler0"Hx% +3 "core" % 0ԍd 2 +55 "sampler0"H% +3 "core" Ȉ% 6ʒ 2 +54 "sampler0"Hv% +3 "core" %K 1{ 2 +54 "sampler0"H% +3 "core" ȓ% 1 2 +54 "sampler0"H% +3 "core" Ȏ% 71 }2 +54 "sampler0"Hw% +3 "core" %o + ~2 +53 "sampler0"H% +11 "core" Ȇ% 2 ~2 +53 "sampler0"H% +3 "core" ȝ%w - 2 +54 "sampler0"H% +4 "core" ȃ% 7F 2 +54 "sampler0"H% +3 "core" ț%H 5Տ ~2 +54 "sampler0"H% +3 "core" Ȑ%Ŧ /ГD 2 +53 "sampler0"H % +4 "core" ȍ% 3َ 2 +54 "sampler0"H% +3 "core" Ȝ%h 4F 2 +54 "sampler0"H% +4 "core" ȏ% 3 2 +53 "sampler0"H+% +3 "core" ȫ%* 3c 2 +54 "sampler0"H % +5 "core" Ȍ%љ 4N 2 +53 "sampler0"H'% +3 "core" ȧ% 3R ~2 +53 "sampler0"H!% +3 "core" ȡ%Q 3 2 +54 "sampler0"H% +3 "core" ȟ%خ 3 2 +53 "sampler0"H% +4 "core" ȗ% ,l ~2 +53 "sampler0"H$% +3 "core" Ȥ%k .ጵ 2 +54 "sampler0"H1% +3 "core" ȱ% .ڐG 2 +53 "sampler0"H%% +3 "core" ȥ%$ +Ќj 2 +54 "sampler0"Hk% +3 "core" % 1ƒD ~2 +54 "sampler0"H8% +3 "core" ȸ% / 2 +54 "sampler0"HX% +3 "core" %F - ~2 +53 "sampler0"H% +3 "core" Ȟ% / 2 +54 "sampler0"H.% +3 "core" Ȯ% 3 2 +53 "sampler0"H% +3 "core" Ț%V ) 2 +55 "sampler0"H0% +3 "core" Ȱ% 3 2 +54 "sampler0"H6% +3 "core" ȶ%" 5 2 +54 "sampler0"HT% +3 "core" % 0 2 +54 "sampler0"H:% +3 "core" Ⱥ% 4 ~2 +53 "sampler0"H5% +6 "core" ȵ%Y 61 }2 +53 "sampler0"H4% +3 "core" ȴ% 6ێg 2 +54 "sampler0"H;% +3 "core" Ȼ% 1ϑn }2 +54 "sampler0"Hf% +5 "core" % 1ǎ ~2 +53 "sampler0"H#% +3 "core" ȣ% 1 2 +54 "sampler0"H<% +3 "core" ȼ%H 3 ~2 +54 "sampler0"H3% +3 "core" ȳ%\ 4 ~2 +54 "sampler0"HE% +3 "core" %  5` 2 +53 "sampler0"HF% +3 "core" %e . 2 +54 "sampler0"HH% +5 "core" % 7( 2 +5 ' +3 "sampler0"H&% +3 "core" Ȧ% 2 +3 ."nadia"Abs.stop +2 "ambient0"J5 +7 ."nadia"PhaseSeparator.stop + "carousel1"G6 +29 "sampler0"H9% +61 "core" ȹ%y +53 "sampler0"HC% +3 "core" %U` +54 "sampler0"HJ% +3 "core" %l +53 "sampler0"HN% +3 "core" %f +54 "sampler0"H % +3 "core" ȋ%[ +55 "sampler0"H?% +3 "core" ȿ%Z +55 "sampler0"HD% +3 "core" %R +54 "sampler0"HM% +5 "core" %f +54 "sampler0"HO% +3 "core" %wn +53 "sampler0"HS% +3 "core" %un +54 "sampler0"HU% +3 "core" %rf +53 "sampler0"HL% +2 "Can"kZ.dz3s=@ +4 "core" %eu +53 "sampler0"HR% +3 "core" %et +54 "sampler0"HV% +3 "core" %`l +54 "sampler0"HZ% +3 "core" %]o +54 "sampler0"HW% +3 "core" %Oi +53 "sampler0"H% +5 "core" ȅ%y +54 "sampler0"H,% +3 "core" Ȭ%} +53 "sampler0"H|% +3 "core" % +53 "sampler0"H(% +3 "core" Ȩ%s +55 "sampler0"HP% +3 "core" % +53 "sampler0"H^% +3 "core" %} +54 "sampler0"H_% +3 "core" %r +54 "sampler0"HK% +3 "core" %q~ +54 "sampler0"Ha% +3 "core" %qm +53 "sampler0"H\\% +4 "core" %[ +53 "sampler0"Hd% +3 "core" %R +55 "sampler0"H@% +5 "core" %Z +53 "sampler0"Hh% +3 "core" %V +54 "sampler0"H`% +3 "core" % +54 "sampler0"HQ% +3 "core" % +54 "sampler0"HG% +3 "core" %P +54 "sampler0"H[% +3 "core" %w} +54 "sampler0"HY% +3 "core" %l +55 "sampler0"Hl% +5 "core" %jP +53 "sampler0"Hm% +3 "core" %d +53 "sampler0"Hq% +3 "core" %Vi +54 "sampler0"Hs% +3 "core" %R +54 "sampler0"Hp% +3 "core" %j +53 "sampler0"Hg% +3 "core" % +54 "sampler0"Ht% +3 "core" % +55 "sampler0"H% +5 "core" ș%v +54 "sampler0"Ho% +3 "core" %q +54 "sampler0"Hn% +3 "core" %l +54 "sampler0"HA% +3 "core" %c +54 "sampler0"Hi% +3 "core" %Z +54 "sampler0"H"% +3 "core" Ȣ%X +53 "sampler0"H}% +3 "core" % +55 "sampler0"H=% +5 "core" Ƚ% +53 "sampler0"H% +3 "core" Ȅ% +54 "sampler0"H% +3 "core" ȑ% +54 "sampler0"H\ % +3 "core" Ȋ% +53 "sampler0"H% +4 "core" Ȃ% +54 "sampler0"H % +3 "core" ȉ%| +54 "sampler0"H]% +3 "core" %v +54 "sampler0"H2% +4 "core" Ȳ%l +54 "sampler0"H% +3 "core" ȁ%d +54 "sampler0"Hc% +3 "core" %: +55 "sampler0"H*% +3 "core" Ȫ%b +54 "sampler0"Hr% +3 "core" %Z +53 "sampler0"H/% +3 "core" ȯ%T +54 "sampler0"H~% +4 "core" % +54 "sampler0"H% +3 "core" % +53 "sampler0"H % +3 "core" Ƞ%{ +54 "sampler0"H-% +3 "core" ȭ%x +54 "sampler0"H% +3 "core" ȇ%l +53 "sampler0"Hu% +3 "core" %g +54 "sampler0"H% +3 "core" ȕ%` +54 "sampler0"H% +3 "core" Ș%Z +54 "sampler0"H% +5 "core" Ȕ%U +54 "sampler0"H% +4 "core" Ȓ%f +54 "sampler0"Hx% +3 "core" % +53 "sampler0"H% +4 "core" Ȉ% +53 "sampler0"Hv% +3 "core" %Ή +53 "sampler0"H% +3 "core" ȓ%p +54 "sampler0"H% +3 "core" Ȏ%q +56 "sampler0"Hw% +4 "core" %a +54 "sampler0"H% +3 "core" Ȇ%e +54 "sampler0"H% +3 "core" ȝ%X +53 "sampler0"H% +4 "core" ȃ%T +54 "sampler0"H% +3 "core" ț% +54 "sampler0"H% +3 "core" Ȑ% +53 "sampler0"H % +3 "core" ȍ%d +54 "sampler0"H% +5 "core" Ȝ%E +54 "sampler0"H% +3 "core" ȏ% +110 "sampler0"H+% +4 "core" ȫ%} +54 "sampler0"H % +3 "core" Ȍ%qi +53 "sampler0"H'% +4 "core" ȧ%n} +54 "sampler0"H!% +3 "core" ȡ%h\\ +55 "sampler0"H% +3 "core" ȟ%hY +53 "sampler0"H% +3 "core" ȗ%a +54 "sampler0"H$% +3 "core" Ȥ%[r +53 "sampler0"H1% +3 "core" ȱ%T +54 "sampler0"H%% +3 "core" ȥ% +53 "sampler0"Hk% +3 "core" %| +54 "sampler0"H8% +3 "core" ȸ% +55 "sampler0"HX% +6 "core" % +54 "sampler0"H% +3 "core" Ȟ%nE +53 "sampler0"H.% +3 "core" Ȯ%# +54 "sampler0"H% +2 "core" Ț% +54 "sampler0"H0% +3 "core" Ȱ%v +54 "sampler0"H6% +3 "core" ȶ%t +59 "sampler0"HT% +3 "core" %ny +54 "sampler0"H:% +3 "core" Ⱥ%f[ +54 "sampler0"H5% +3 "core" ȵ%Zt +54 "sampler0"H4% +3 "core" ȴ%ZI +53 "sampler0"H;% +3 "core" Ȼ%V\\ +54 "sampler0"Hf% +3 "core" % +54 "sampler0"H#% +3 "core" ȣ%k +53 "sampler0"H<% +3 "core" ȼ% +55 "sampler0"H3% +3 "core" ȳ% +54 "sampler0"HE% +3 "core" % +54 "sampler0"HF% +2 "core" % +54 "sampler0"HH% +3 "core" %t +54 "sampler0"H&% +5 "core" Ȧ%v +53 "sampler0"H9% +3 "core" ȹ%i +54 "sampler0"HC% +3 "core" %f +55 "sampler0"HJ% +4 "core" %a +53 "sampler0"HN% +3 "core" %` +54 "sampler0"H>% +3 "core" Ⱦ% +53 "sampler0"H7% +3 "core" ȷ%Q +54 "sampler0"H % +3 "core" ȋ% +53 "sampler0"H?% +3 "core" ȿ% +54 "sampler0"HD% +3 "core" % +55 "sampler0"HM% +5 "core" %y +53 "sampler0"HO% +3 "core" %p +54 "sampler0"HS% +3 "core" %f +54 "sampler0"HU% +3 "core" %d +53 "sampler0"HL% +3 "core" %Z +55 "sampler0"HR% +3 "core" %U +53 "sampler0"HV% +3 "core" %T +56 "sampler0"HZ% +4 "core" % +54 "sampler0"HW% +4 "core" % +53 "sampler0"H% +3 "core" ȅ% +54 "sampler0"H,% +3 "core" Ȭ% +53 "sampler0"H|% +3 "core" % +54 "sampler0"H(% +3 "core" Ȩ% +54 "sampler0"HP% +3 "core" %t +54 "sampler0"H^% +5 "core" %v +54 "sampler0"H_% +3 "core" %m +54 "sampler0"HK% +3 "core" %j +53 "sampler0"Ha% +4 "core" %] +54 "sampler0"H\\% +3 "core" %b +54 "sampler0"Hd% +3 "core" %W +53 "sampler0"H@% +3 "core" % +55 "sampler0"Hh% +4 "core" % +54 "sampler0"H`% +3 "core" %Љ +53 "sampler0"HQ% +3 "core" %҉ +54 "sampler0"HG% +3 "core" %u +53 "sampler0"H[% +3 "core" %i +54 "sampler0"HY% +4 "core" %g +53 "sampler0"Hl% +3 "core" %` +56 "sampler0"Hm% +4 "core" %\\ +54 "sampler0"Hq% +3 "core" %W +53 "sampler0"Hs% +4 "core" %S +54 "sampler0"Hp% +3 "core" % +54 "sampler0"Hg% +6 "core" % +55 "sampler0"Ht% +3 "core" % +54 "sampler0"H% +3 "core" ș%z +53 "sampler0"Ho% +4 "core" %k +54 "sampler0"Hn% +4 "core" %i +53 "sampler0"HA% +3 "core" %b +53 "sampler0"Hi% +3 "core" %Z +54 "sampler0"H"% +3 "core" Ȣ%X +53 "sampler0"H}% +3 "core" %V +54 "sampler0"H=% +3 "core" Ƚ%㉊ +54 "sampler0"H% +3 "core" Ȅ% +54 "sampler0"H% +3 "core" ȑ%| +53 "sampler0"H\ % +3 "core" Ȋ%v +116 "sampler0"H% +3 "core" Ȃ%h +53 "sampler0"H % +3 "core" ȉ%a +54 "sampler0"H]% +3 "core" %e +53 "sampler0"H2% +3 "core" Ȳ%\\ +54 "sampler0"H% +3 "core" ȁ%‰ +54 "sampler0"Hc% +3 "core" % +54 "sampler0"H*% +3 "core" Ȫ% +53 "sampler0"Hr% +4 "core" % +54 "sampler0"H/% +3 "core" ȯ% +54 "sampler0"H~% +3 "core" %} +54 "sampler0"H% +3 "core" % +54 "sampler0"H % +3 "core" Ƞ%~ +54 "sampler0"H-% +3 "core" ȭ%x +53 "sampler0"H% +4 "core" ȇ%s +53 "sampler0"Hu% +3 "core" %l +54 "sampler0"H% +3 "core" ȕ%n +53 "sampler0"H% +4 "core" Ș%` +55 "sampler0"H% +4 "core" Ȕ%^ +54 "sampler0"H% +4 "core" Ȓ%Z +53 "sampler0"Hx% +3 "core" % +53 "sampler0"H% +3 "core" Ȉ% +54 "sampler0"Hv% +3 "core" % +54 "sampler0"H% +3 "core" ȓ%} +54 "sampler0"H% +3 "core" Ȏ%t +55 "sampler0"Hw% +4 "core" %v +53 "sampler0"H% +4 "core" Ȇ%n} +54 "sampler0"H% +3 "core" ȝ%f +53 "sampler0"H% +3 "core" ȃ%Z +54 "sampler0"H% +3 "core" ț%M +53 "sampler0"H% +3 "core" Ȑ% +54 "sampler0"H % +3 "core" ȍ% +55 "sampler0"H% +5 "core" Ȝ% +54 "sampler0"H% +3 "core" ȏ%~ +54 "sampler0"H+% +3 "core" ȫ%u +53 "sampler0"H % +4 "core" Ȍ% +54 "sampler0"H'% +3 "core" ȧ%l +54 "sampler0"H!% +3 "core" ȡ%c +53 "sampler0"H% +3 "core" ȟ%\\ +56 "sampler0"H% +3 "core" ȗ%T +53 "sampler0"H$% +3 "core" Ȥ%P +54 "sampler0"H1% +3 "core" ȱ% +54 "sampler0"H%% +3 "core" ȥ% +53 "sampler0"Hk% +3 "core" % +54 "sampler0"H8% +4 "core" ȸ%x +54 "sampler0"HX% +3 "core" %o +55 "sampler0"H% +4 "core" Ȟ%l +53 "sampler0"H.% +3 "core" Ȯ%e +53 "sampler0"H% +3 "core" Ț%a +54 "sampler0"H0% +3 "core" Ȱ%W +54 "sampler0"H6% +3 "core" ȶ%Q +20 "Can"k[.gz/s>GS +33 "sampler0"HT% +3 "core" % +55 "sampler0"H:% +6 "core" Ⱥ%^ +54 "sampler0"H5% +4 "core" ȵ% +54 "sampler0"H4% +3 "core" ȴ% +53 "sampler0"H;% +3 "core" Ȼ% +54 "sampler0"Hf% +3 "core" % +53 "sampler0"H#% +3 "core" ȣ%x +54 "sampler0"H<% +3 "core" ȼ%o +53 "sampler0"H3% +3 "core" ȳ%n +55 "sampler0"HE% +5 "core" %j +54 "sampler0"HF% +3 "core" %j +54 "sampler0"HH% +3 "core" %` +54 "sampler0"H&% +3 "core" Ȧ%Z +53 "sampler0"H9% +3 "core" ȹ%U +54 "sampler0"HC% +3 "core" % +54 "sampler0"HJ% +3 "core" % +55 "sampler0"HN% +8 "core" % +56 "sampler0"H>% +3 "core" Ⱦ% +53 "sampler0"H7% +3 "core" ȷ%| +54 "sampler0"H % +4 "core" ȋ%n +54 "sampler0"H?% +4 "core" ȿ%k +54 "sampler0"HD% +4 "core" %\\ +54 "sampler0"HM% +4 "core" %W +53 "sampler0"HO% +3 "core" %Q +54 "sampler0"HS% +3 "core" %P +53 "sampler0"HU% +5 "core" % +55 "sampler0"HL% +3 "core" % +56 "sampler0"HR% +7 "core" % +57 "sampler0"HV% +9 "core" %s +81 #"Benjamin"Session Begun +18 "sampler0"HZ% +3 "core" %c +54 "sampler0"HW% +3 "core" %gZ +53 "sampler0"H% +3 "core" ȅ%^ +72 "sampler0"H,% +5 "core" Ȭ%U +15 #"nadia"-> BACsampler.prime 50,nil +19 "carousel0"F|% +7 "core" %0חKP \  +4 #"nadia"Priming sample loop w/50ml +4 .SamplePump.setPosition! 0ml +6 "sampler0"H7 +7 ."nadia"Exhaust.close +4 "sampler0"H(0 +8 HP% +3 "core" % +54 "sampler0"H^% +3 "core" %ډ +54 "sampler0"H_% +3 "core" %։ +31 Ȩ0 +2 ."nadia"Bypass.open +2 "sampler1"IK1 +22 "sampler0"Ha% +3 "core" %b +54 "sampler0"H\\% +4 "core" %[ +54 "sampler0"Hd% +3 "core" %T +15 1 +39 "sampler0"H@% +3 "core" % +32 "ambient0"Jh% +3 "core" % +4 "Can"k[.gz/s>TH +2 "ambient0"J`% +5 "core" %\ +2 ."nadia"Exhaust.open +2 "sampler0"HQ2 +12 HG% +3 "core" %n +53 "sampler0"H[% +3 "core" %z +54 "sampler0"HY% +3 "core" % +22 2 +32 "sampler0"Hl% +3 "core" %剼 +39 ."closeBypass"Bypass.close +3 "sampler1"Im/ +11 "sampler0"Hq% +4 "core" %+ +53 "sampler0"Hs% +3 "core" %$ +54 "sampler0"Hp% +4 "core" % +25 / +28 "sampler0"Hg% +4 "core" % +41 "sampler0"Ht% +3 "core" % +2 "ambient0"J% +3 "core" ʙ% +3 ."nadia"Abs.in :default +3 "ambient0"J>9 e  +10 ."nadia"Abs.hold +3 "ambient0"Jo+ +7 "core" + +5 ."nadia"PhaseSeparator.hold +4 "carousel1"GA, +3 "sampler0"Hn% +13 "core" , + % +4 "ambient0"Ji% +11 "core" % +3 "sampler0"H"% +6 "core" Ȣ%~ +2 "sampler0"H}% +5 "core" % +4 ."nadia"SamplePump.in :start +2 "sampler0"H>^ }n@#(@y3 22Q +10 ."nadia"SamplePump.seek 50ml,3:50 +5 "sampler0"H=' +9 H% +3 "core" Ȅ%z 2 +41 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n@#(@y3 22Q +10 H% +3 "core" ȑ%r +k2 2 +54 "sampler0"H\ % +3 "core" Ȋ%  ~ 2 +54 "sampler0"H% +3 "core" Ȃ%2  2 +54 "sampler0"H % +3 "core" ȉ% "` }2 +54 "sampler0"H]% +4 "core" %\ '8 2 +53 "sampler0"H2% +4 "core" Ȳ% X 'u 2 +56 "sampler0"H% +3 "core" ȁ% 0ٓ ~2 +54 "sampler0"Hc% +4 "core" %6 *ԏ ~2 +54 "sampler0"H*% +3 "core" Ȫ% *ȑ^ 2 +54 "sampler0"Hr% +3 "core" % +ŏ7 2 +53 "sampler0"H/% +3 "core" ȯ%a )~ 2 +54 "sampler0"H~% +3 "core" % , 2 +54 "sampler0"H% +7 "core" %: * ~2 +54 "sampler0"H % +4 "core" Ƞ% - 2 +54 "sampler0"H-% +3 "core" ȭ%# *< ~2 +54 "sampler0"H% +3 "core" ȇ%%} #L 2 +54 "sampler0"Hu% +3 "core" %' ' 2 +54 "sampler0"H% +3 "core" ȕ%*? . 2 +54 "sampler0"H% +3 "core" Ș%, .s }2 +55 "sampler0"H% +3 "core" Ȕ%/ 2ړf 2 +54 "sampler0"H% +3 "core" Ȓ%1o -̏ 2 +54 "sampler0"Hx% +3 "core" %3 5ΓF 2 +53 "sampler0"H% +4 "core" Ȉ%6/ -h 2 +54 "sampler0"Hv% +3 "core" %8 + 2 +53 "sampler0"H% +3 "core" ȓ%: ,Z ~2 +54 "sampler0"H% +3 "core" Ȏ%=Q 3 2 +55 "sampler0"Hw% +4 "core" %? +^ 2 +53 "sampler0"H% +3 "core" Ȇ%B 3 ~2 +54 "sampler0"H% +3 "core" ȝ%D} 3 ~2 +54 "sampler0"H% +3 "core" ȃ%F # 2 +54 "sampler0"H% +3 "core" ț%I> - 2 +53 "sampler0"H% +4 "core" Ȑ%K 0ڋ ~2 +54 "sampler0"H % +3 "core" ȍ%N *͑7 2 +54 "sampler0"H% +5 "core" Ȝ%Pv +ɍP }2 +54 "sampler0"H% +3 "core" ȏ%R 8W ~2 +54 "sampler0"H+% +3 "core" ȫ%U< . ~2 +53 "sampler0"H % +3 "core" Ȍ%W 0 2 +54 "sampler0"H'% +3 "core" ȧ%Y 4 2 +53 "sampler0"H!% +4 "core" ȡ%\\R 3 ~2 +53 "sampler0"H% +3 "core" ȟ%^ 5 2 +113 "sampler0"H% +4 "core" ȗ%c 4 }2 +53 "sampler0"H$% +3 "core" Ȥ%e 3 2 +54 "sampler0"H1% +3 "core" ȱ%hH ( 2 +54 "sampler0"H%% +3 "core" ȥ%j /ؓ 2 +54 "sampler0"Hk% +3 "core" %m 1 2 +54 "sampler0"H8% +3 "core" ȸ%om .Ñ ~2 +55 "sampler0"HX% +4 "core" %q /‘ 2 +54 "sampler0"H% +3 "core" Ȟ%tI . 2 +54 "sampler0"H.% +3 "core" Ȯ%v -$ 2 +54 "sampler0"H% +4 "core" Ț%y .b 2 +53 "sampler0"H0% +3 "core" Ȱ%{r 'Q 2 +54 "sampler0"H6% +3 "core" ȶ%} (* 2 +54 "sampler0"HT% +4 "core" %0 7 2 +54 "sampler0"H:% +3 "core" Ⱥ% 0 2 +54 "sampler0"H5% +3 "core" ȵ% 7 2 +54 "sampler0"H4% +3 "core" ȴ%] 4T ~2 +54 "sampler0"H;% +3 "core" Ȼ% 1 ~2 +54 "sampler0"Hf% +3 "core" %" /ޏ 2 +54 "sampler0"H#% +4 "core" ȣ% 2ۑ 2 +54 "sampler0"H<% +3 "core" ȼ% .ёF 2 +54 "sampler0"H3% +3 "core" ȳ%Q 7ϓY 2 +53 "sampler0"HE% +3 "core" % 3!x 2 +54 "sampler0"HF% +3 "core" % .3 2 +54 "sampler0"HH% +3 "core" %i 3= ~2 +53 "sampler0"H&% +3 "core" Ȧ% 7 2 +54 "sampler0"H9% +3 "core" ȹ%# 0m 2 +53 "sampler0"HC% +3 "core" %} ,Q 2 +56 "sampler0"HJ% +5 "core" % 0 2 +55 "sampler0"HN% +3 "core" %s / 2 +54 "sampler0"H>% +3 "core" Ⱦ% 3 2 +54 "sampler0"H7% +3 "core" ȷ%4 + 2 +53 "sampler0"H % +3 "core" ȋ% 2 2 +54 "sampler0"H?% +3 "core" ȿ% 5m 2 +53 "sampler0"HD% +3 "core" %H /ܔ 2 +56 "sampler0"HM% +4 "core" % 3͐ ~2 +53 "sampler0"HO% +3 "core" % 3ő 2 +54 "sampler0"HS% +3 "core" % .B ~2 +53 "sampler0"HU% +3 "core" % 0 2 +53 "sampler0"HL% +4 "core" %3 3Z 2 +53 "sampler0"HR% +3 "core" % 1 2 +54 "sampler0"HV% +3 "core" % 4c ~2 +55 "sampler0"HZ% +5 "core" %m , ~2 +54 "sampler0"HW% +3 "core" % - }2 +54 "sampler0"H% +3 "core" ȅ%3 . 2 +54 "sampler0"H,% +3 "core" Ȭ%̙ 1ԓ= 2 +54 "sampler0"H|% +4 "core" % +ƍ& 2 +54 "sampler0"HP% +3 "core" %d 7 ~2 +53 "sampler0"H^% +3 "core" %ӿ - 2 +55 "sampler0"H_% +3 "core" %+ . 2 +54 "sampler0"H(% +3 "core" Ȩ%؇ 3 }2 +53 "sampler0"Ha% +4 "core" % : ~2 +53 "sampler0"H\\% +3 "core" %E /b ~2 +54 "sampler0"Hd% +4 "core" %ߢ 8 ~2 +53 "sampler0"HK% +3 "core" % 3 2 +54 "sampler0"H@% +4 "core" %w 4鑋 2 +55 "sampler0"Hh% +4 "core" % .) 2 +53 "sampler0"H`% +4 "core" %H .ْ 2 +54 "sampler0"HG% +3 "core" % 2ݓ 2 +53 "sampler0"H[% +3 "core" % .͏ ~2 +53 "sampler0"HY% +4 "core" %a / ~2 +53 "sampler0"HQ% +3 "core" % / ~2 +54 "sampler0"Hl% +3 "core" %! 1~ ~2 +56 "sampler0"Hq% +3 "core" % 5 2 +54 "sampler0"Hs% +3 "core" % . 2 +54 "sampler0"Hp% +3 "core" %X / 2 +54 "sampler0"Hm% +3 "core" % : ~2 +53 "sampler0"Hg% +3 "core" % 1 2 +54 "sampler0"Ht% +3 "core" %w ,ϓ 2 +54 "sampler0"H% +3 "core" ș% ;̎} ~2 +55 "sampler0"Hn% +4 "core" %K 7 2 +54 "sampler0"Hi% +3 "core" %\ 1O 2 +54 "sampler0"H"% +3 "core" Ȣ%  5u ~2 +54 "sampler0"H}% +4 "core" %z 1{ 2 +53 "sampler0"H% +3 "core" Ȅ% 2 2 +6 Ƚ' +3 "sampler0"H% +3 "core" ȑ% 2 +3 ."nadia"Abs.stop +2 "ambient0"J5 +8 ."nadia"PhaseSeparator.stop +2 "carousel1"G6 +28 "sampler0"H\ % +3 "core" Ȋ%ae +54 "sampler0"H% +3 "core" Ȃ%\\L +54 "sampler0"H % +4 "core" ȉ%ZS +54 "sampler0"H]% +3 "core" %W +53 "sampler0"HB% +3 "core" %j +54 "sampler0"H2% +3 "core" Ȳ%f +111 "sampler0"H% +4 "core" ȁ%vP +54 "sampler0"Hc% +3 "core" %oq +54 "sampler0"H*% +3 "core" Ȫ%fl +54 "sampler0"Hr% +3 "core" %dI +54 "sampler0"H/% +3 "core" ȯ%Y +54 "sampler0"H~% +4 "core" %W2 +54 "sampler0"H% +3 "core" % +54 "sampler0"H % +3 "core" Ƞ% +54 "sampler0"H-% +4 "core" ȭ% +54 "sampler0"H% +5 "core" ȇ% +53 "sampler0"Hu% +3 "core" %r +53 "sampler0"H% +3 "core" ȕ%o +54 "sampler0"H% +3 "core" Ș%f +54 "sampler0"H% +3 "core" Ȕ%f| +54 "sampler0"H% +3 "core" Ȓ%Z +53 "sampler0"Hx% +3 "core" %y +51 !"na"o:UserAbort : mesg"User Abort!!:@timestampu: Time z:@checkpoint: omitted:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'"'na aborter-:1:in `topLevelBinding'"TWtR +11956 kZ.qz4s=^p +7253 #"nadia"-> Power.sampler :OFF +7 .Gate.power :sampler,:OFF +4658 #-> ESP.shutdown +6 "sampler0"H% +5 "core" Ȉ% +4 ."closeIntake"Intake.close +4 "sampler0"Hv/ +10 ."closeExhaust"Exhaust.close +4 "sampler0"H0 +7 "Can"kZ.rz0t8d3 +126 "core" / +25 ȓ0 +10 ."nadia"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +7 .Gate.power :core,:OFF +7 .Gate.power :main,:OFF +4754 #"Benjamin"Session Ended +7024 "Can"lZ.jz2td +11955 kZ8._z%teY +11962 kZ.^z+te +6470 #"nadia"-> slot 51 +801 #-> slot 51=>:dry +2416 #-> start {fire archive,lysis} +22 ."detached from nadia#3"Gate.power :sampler,:OFF,limit to 3500mA +6 .Gate.power :skimmer,:OFF,limit to 3500mA +10 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +12 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 D +3 "core" Ď +7 ."detached from nadia#3"Plunge.configure :default +2 "valve0"D# P ޤ-2 +11 ."detached from nadia#3"SamplePump.configure :default + "sampler0"H#^ #(22 +14 ."detached from nadia#3"Toroid.configure :default +2 "valve0"D$ ֜2 +10 ."detached from nadia#3"Carousel.configure :locked +2 "carousel0"F#` F 2\ 2 +10 ."detached from nadia#3"Abs.configure :default +2 "ambient0"J#9 e  +9 ."detached from nadia#3"PhaseSeparator.configure :full +2 "carousel1"G$ +9 ."detached from nadia#3"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."detached from nadia#3"Air.configure :default +2 "syringe1"C#d P]z<2 +7 ."detached from nadia#3"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from nadia#3"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +10 ."detached from nadia#3"PinPort.configure :default +2 "valve1"E$ B22 +8 #"detached from nadia#3"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +10 "valve0"Dw% +3 D& +3 "carousel0"F% +5 "syringe0"B& +5 "syringe1"C% +5 C& +3 "syringe0"B % +5 "valve1"E& +9 "core" %GhOP < +3 Ć&OK  +21 ."Plunge_home"Plunge.home.jog 0 +13 "valve0"D) +3 ."Toroid_home"Toroid.home.jog 0 +7 "valve0"D+* +12 "core" Ɲ%ז \ * +2 ƒ&^  + Û%%  + Ð&( $ + %٩%  + Ŝ&{OQ , +10 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +5 ."Carousel_home"Carousel.in :default +6 ."PinPort_home"PinPort.home.jog 0 +6 ."Air_home"Air.home.jog 0 +7 "syringe0"B ) +2 "syringe1"C'* +8 "syringe0"B!* +4 "carousel0"F><` F 22 +5 "valve1"E* +3 "syringe1"C) +5 ."Carousel_home"Carousel.home.jog 0 +11 "carousel0"F$) +6 "core" ď) + ī* + Œ) + ç* + ¡* + ×) ş* +3 "syringe0"B%% +9 "syringe1"Ck& +6 "valve1"E1& +4 "syringe0"B8& +6 "syringe1"CX% +8 "valve0"D.& +4 D% +8 "core" Ƥ) + ¥%t٩d + &#  + ű& _O=  ¸&u,_ + %% + Į&O? + Ğ%_GvO7 +10 ."Lysis2_home"Lysis2.in :homing +8 ."Lysis1_home"Lysis1.in :homing +6 ."Archive_home"Archive.in :homing +6 ."Plunge_home"Plunge.in :homing +10 ."PinPort_home"PinPort.in :homing +5 ."Air_home"Air.in :homing +5 ."Toroid_home"Toroid.in :homing +6 "syringe0"B> (2 +3 "syringe1"C? (2 +65 "syringe0"B? -2 +4 "valve0"D> (2 +4 "valve1"E?2 +2 "syringe1"C> -2 +3 "valve0"D?2 +6 ."Lysis2_home"Lysis2.jog 1618 +24 ."Lysis1_home"Lysis1.jog 2267 +9 ."Air_home"Air.jog 1243 +7 ."Toroid_home"Toroid.jog 1362 +7 ."Archive_home"Archive.jog 1615 +7 ."PinPort_home"PinPort.jog 1223 +6 ."Plunge_home"Plunge.jog 1463 +7 "syringe0"B)R +3 "syringe1"C0* +2 C6) +9 "valve0"DT*R +4 "syringe0"B:*O +3 "valve1"E5* +5 "valve0"D4) +96 "core" ö) +3 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +8 ."Air_home"Air.seek :home +3 "syringe1"C;' +8 "core" š) + û' +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 ."Air_home"Air.in :default +9 "syringe0"B72 +5 "syringe1"C>d P]z<2 +4 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"Bf' +10 "core" º* + Ĵ) + ð* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Archive_home"Archive.setRawPosition! 50 +5 ."Plunge_home"Plunge.setRawPosition! 50 +4 "syringe1"C82 +5 "syringe0"B82 +7 "valve0"D72 +4 ."Lysis1_home"Lysis1.seek :home +3 ."Archive_home"Archive.seek :home +11 "syringe1"C#( +7 "syringe0"B<( +8 ."Plunge_home"Plunge.seek :home +10 "valve0"D3' +6 "core" ' +2 ŵ* + ã( +5 ."Lysis2_home"Lysis2.in :default +4 ."Lysis1_home"Lysis1.in :default +6 ."PinPort_home"PinPort.setRawPosition! 3150 +5 "syringe0"B>d P\\k<2 +3 "syringe1"C?d P[<2 +2 "valve1"E8 N +15 ."PinPort_home"PinPort.seek :home +7 "valve1"EE( +8 "core" ¼( + ij' +2 ."Plunge_home"Plunge.in :default +3 ."Archive_home"Archive.in :default +5 "valve0"D> P ޤ-2 +6 "syringe0"B?d PZ<2 +140 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +8 ."Toroid_home"Toroid.seek :home +3 "valve0"DF( +8 "core" ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +242 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +37 ."clearToroid"Toroid.seek :clear +4 "valve1"E&& +6 "valve0"DH( +5 "core" Ŧ&O` . +5 ."detached from nadia#3"PinPort.in :unlocking +2 "valve1"E? B2 +9 ."detached from nadia#3"PinPort.seek :clear +6 "valve1"E9( +45 "core" Ź( +282 ( +3 ."detached from nadia#3"Air.seek :clear +3 "syringe1"CC' +10 "core" ' +3 ."detached from nadia#3"Lysis1.seek :clear +2 "syringe1"CJ( +71 "core" ( +2 ."detached from nadia#3"Lysis2.seek :clear +5 "syringe0"BN'j +78 "core" ' +2 ."detached from nadia#3"Archive.seek :clear +4 "syringe0"B>(j +9 "Can"lY.[z4s8 g> +69 "core" ¾( +2 ."detached from nadia#3"Carousel.in :default +2 "carousel0"F><` F 22 +10 F7% +4 "core" Ʒ%0Qז  +3 ."detached from nadia#3"Carousel.home.seek 51 +4 "carousel0"F '0 +17 "core" Ƌ' +3 "valve0"D?% +4 "core" Ŀ%^OV !i +3 #"detached from nadia#3"Selecting Cartridge 51 +2 "valve0"DD% +11 "core" %^OS !t +3 "syringe1"CM& +3 "core" &وM +3 "syringe1"CO% +4 "core" %ݯ7 +2 "valve1"ES& +4 "core" &vOQ  +4 ."detached from nadia#3"Carousel.seek 51 +6 "carousel0"FU'0 +15 "core" ' +4 "carousel0"FL% +4 "core" %0.כv 2 +3 ."detached from nadia#3"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +8 "syringe1"CR& +3 "core" &مL +3 ."detached from nadia#3"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from nadia#3"Lysis1.seek :locked +3 "syringe1"CV( +252 "core" ( +4 "valve0"DZ% +9 "core" %^O8 !q +4 ."Plunge"Plunge.seek :locked +3 "valve0"DW'Z +8 "syringe1"C% +9 "core" Å%ݰ>> +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C,'6 +79 "core" ' +60 ì' +13 ."detached from nadia#3"Carousel.reconfigure :locked +4 "carousel0"F#` F 2\ 2 +11 F|% +3 "core" %0Pכ/  +8 #"detached from nadia#3"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."detached from nadia#3"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +4 "cartridge_broadcast"~ +11 ."detached from nadia#3"Gate.power :cartridge,:OFF +7 #Mounted archive Cartridge +9 "valve0"DP% +4 "core" %ZG*OL 2 +3 "syringe1"C^& +3 "core" &% +3 "syringe1"C_% +5 "core" %6u| t +3 "carousel0"F(% +4 "core" ƨ%0ךW \ +3 !"detached from Benjamin"o:Cmd::Error: mesg"(Cannot process while in READY state:@timestampu: Time ;:bt[">/home/esp/esp2/utils/auv/cmd.rb:467:in `startProcessing!'"5/home/esp/esp2/utils/auv/cmd.rb:536:in `process'":/home/esp/esp2/utils/auv/cmd.rb:172:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"$Benjamin:1:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6 #"FILTERING"Preparing to filter archiveHiBiomass_bac cartridge in slot 51 +7 #"detached from Benjamin"Waiting to Resume from Checkpoint "Selecting Cartridge 51" +3 "valve0"Da% +8 "core" %ZG<OE +2 "syringe1"C\\& +4 "core" &# +3 "syringe1"Cd% +5 "core" %6~ l +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +6 "carousel1"GK& +9 "core" & +40 "carousel1"G@& +3 "core" & +47 "carousel1"Gh& +3 "core" &d +47 "carousel1"G`& +3 "core" &p +47 "carousel1"GG& +3 "core" &v +47 "carousel1"G[& +4 "core" &lX +46 "carousel1"GY& +3 "core" &o +47 "carousel1"GQ& +3 "core" &U +48 "carousel1"Gl& +3 "core" & +47 "carousel1"Gq& +6 "core" &s +44 "carousel1"Gs& +3 "core" &a +47 "carousel1"Gp& +3 "core" &n +47 "carousel1"Gm& +3 "core" & +47 "carousel1"Gg& +3 "core" &o +4 "Can"lY.[z4t6pg\ g + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 27C (drifted 1%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +10 .PhaseSeparator.in :filling + "carousel1"G?>> +7 ."FILTERING"PhaseSeparator.hold +3 "carousel1"Gt, +6 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +718 "core" ,H +7 #"FILTERING"Head pressure reached 3.22psi in 8 seconds +2 "carousel0"F% +8 "core" ƙ%0כH \ +4 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +8 ."FILTERING"Bypass.open +3 "sampler1"In1 +155 "core" 1 +74 "ambient0"Ji% +4 "core" % +5 "Can"lY.[z4s>8hw +2 "ambient0"J"% +4 "core" ʢ% +3 ."FILTERING"Intake.open +2 "sampler0"H}1 +150 "core" 1 +73 "sampler0"H% +4 "core" Ȅ%9 +3 "ambient0"J% +4 "core" ʑ%y +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"J\ + +6 "core" ʊ+ +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G, +7 "core" ǂ, +3 "sampler0"H % +4 "core" ȉ%2 +3 "sampler0"H]% +5 "core" %5 +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "17:46:11.24" +2063 #"bill"Session Begun +1372 #-> exit +3 #Session Ended +123 #"nadia"-> Head.psi +3 "carousel1"GB& +7 "core" &pL +1135 "Can"lY.[z4s?"k +4 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n@#(@;y3 22Q +10 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"Ho'6 +54 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n@#(@;y3 22Q +1288 "core" ' +3 "sampler0"HA% +6 "core" %6 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +8 ."FILTERING"Exhaust.open +2 "sampler0"H22 +150 "core" Ȳ2 +74 ."closeBypass"Bypass.close +3 "sampler1"I/ +154 "core" Ɂ/ +74 "sampler0"Hc% +3 "core" %Bt +4 "ambient0"J*% +67 "core" ʪ% +2 ."FILTERING"Abs.in :default +5 "ambient0"J>9 e  +9 ."FILTERING"Abs.hold +2 "ambient0"Jr+ +5 "core" + +4 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G/, +7 "core" ǯ, +2 "ambient0"J~% +4 "core" % +4 "sampler0"H% +5 "core" %^ }n@#(@y3 22Q +9 ."FILTERING"SamplePump.seek 200ml,13:50 +3 "sampler0"H-'G +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n@#(@y3 22Q +2107 "Can"lYa.cz&sDYo$ +11960 lZ.rz2sSVyKB +11958 jZ.zsRV}` +194 "core" ȭ' +3 "sampler0"H% +5 "core" ȇ%Gq 2 +3 ."FILTERING"Abs.stop +4 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"Iu1 +155 "core" 1 +3 ."FILTERING"Intake.close +2 "sampler0"H/ +149 "core" ȕ/ +2 "carousel0"F% +5 "core" Ƙ%0כ}I \ +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :crack +2 "valve0"D( +170 "core" Ĕ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"D(m +210 "core" Ē( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +9 ."FILTERING"Toroid.seek :clear +3 "valve0"Dx( +372 "core" ( +3 "ambient0"J% +4 "core" ʈ% +4 "Can"jZ.zstHc + "ambient0"Jv% +5 "core" % +2 "ambient0"J% +4 "core" ʓ% +3 #"FILTERING"Pressurizing cartridge to 26psi above 14.6psia +3 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +7 ."FILTERING"Intake.open +2 "sampler0"H1 +150 "core" Ȏ1 +3 ."FILTERING"Bypass.close +3 "sampler1"Iw/ +149 "core" / +2 ."FILTERING"Exhaust.close +7 "sampler0"H0 +150 "core" Ȇ0 +3 "sampler0"H % +3 "core" ȍ% +3 "ambient0"J% +4 "core" ʐ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"J+ +7 "core" ʛ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +9 "core" ǃ, +3 "sampler0"H% +4 "core" ȝ%≎ +3 "sampler0"H% +4 "core" Ȝ%Xs +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>n@u03 22Q +9 ."FILTERING"SamplePump.seek 2.3ml,40 seconds +9 "sampler0"H%' +505 "core" ȥ'/' 2 +4 "sampler0"Hk% +4 "core" %, 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"Intake.close +3 "sampler0"HX/ +149 "core" / +2 "ambient0"J8% +5 "core" ʸ% +3 #"FILTERING"Pressurized cartridge with 0.106ml to 26.2psi +2 #Isolated @36.8psia (22.2psi) +7 #Verifying cartridge holds at least 0.65..0.85 of 36.8psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +6 "ambient0"J.% +9 "core" ʮ% +41 "ambient0"J% +4 "core" ʞ% +46 "ambient0"J1% +4 "core" ʱ%T +46 "ambient0"Jj% +3 "core" %2 +47 "ambient0"J{% +4 "core" % +46 "ambient0"Jy% +4 "core" % +46 "ambient0"Je% +4 "core" % +46 "ambient0"Jz% +4 "core" % +46 "ambient0"J)% +5 "core" ʩ% +45 "ambient0"JI% +4 "core" % +47 "ambient0"Jb% +4 "core" % +46 "ambient0"J % +3 "core" ʌ%o +3 #"FILTERING"Cartridge pressure fell 11% to 32.7psia +3 "carousel0"F'% +9 "core" Ƨ%0כ{I \ +68 #"FILTERING"Sampling 306.1ml +3 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +7 ."FILTERING"Exhaust.open +2 "sampler0"H2 +151 "core" ȗ2 +3 ."FILTERING"Intake.open +2 "sampler0"H+1 +149 "core" ȫ1 +4 "Can"jZ.zsrJiJ +2 "ambient0"J!% +5 "core" ʡ%| +4 "ambient0"J% +4 "core" ʟ%L +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"J+ +9 "core" ʏ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G6, +8 "core" Ƕ, +3 "ambient0"J% +4 "core" ʚ% +4 "sampler0"H0% +5 "core" Ȱ%:  +2 "sampler0"H:% +4 "core" Ⱥ%҉  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n@#(@@3 22Q +11 ."FILTERING"SamplePump.seek 306.1ml,20:55 +5 "sampler0"H4', +51 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d n@#(@@3 22Q +227 H5% +3 "core" ȵ% \ _\\ 2 +3 "ambient0"J&% +4 "core" ʦ%u { +2 #"sampling"Cartridge 51 filtering 2/306ml at 0.58ml/s with 25.1psi vacuum +5993 "sampler0"HT% +4 "core" %a 4 2 +3 "ambient0"JF% +5 "core" % | + #"sampling"Cartridge 51 filtering 39/306ml at 0.62ml/s with 20.7psi vacuum +1870 "Can"kYf.zsQV~ +4117 "sampler0"HE% +4 "core" %7  2 +2 "ambient0"J7% +4 "core" ʷ%m% } +3 #"sampling"Cartridge 51 filtering 75/306ml at 0.59ml/s with 14.6psi vacuum 8.3m deep (27.0psia) +5987 "sampler0"H?% +3 "core" ȿ%SS 2 +3 "ambient0"J;% +4 "core" ʻ%v { +2 #"sampling"Cartridge 51 filtering 109/306ml at 0.57ml/s with 26.8psi vacuum +1825 "Can"iYu.zsRVP/ +4164 "sampler0"HD% +3 "core" %"\ ږ 2 +3 "ambient0"J#% +4 "core" ʣ%l } +2 #"sampling"Cartridge 51 filtering 141/306ml at 0.53ml/s with 16.6psi vacuum 7.5m deep (25.8psia) +5987 "sampler0"HM% +4 "core" %O}N 2 +3 "ambient0"JO% +3 "core" %w  +3 #"sampling"Cartridge 51 filtering 171/306ml at 0.50ml/s with 26.4psi vacuum +1781 "Can"kYa.zs_TA? +4206 "sampler0"HS% +4 "core" %CE 2 +2 "ambient0"JL% +5 "core" %\\5 } +2 #"sampling"Cartridge 51 filtering 199/306ml at 0.48ml/s with 24.6psi vacuum +5987 "sampler0"HR% +4 "core" %ؔ9 2 +3 "ambient0"Jf% +3 "core" %vu +3 #"sampling"Cartridge 51 filtering 226/306ml at 0.45ml/s with 27.1psi vacuum +1737 "Can"lX.z1sbS3< +4250 "sampler0"H<% +4 "core" ȼ%d 2 +3 "ambient0"J$% +3 "core" ʤ%V +3 #"sampling"Cartridge 51 filtering 252/306ml at 0.43ml/s with 22.9psi vacuum +5987 "sampler0"H % +3 "core" ȋ%vfu 2 +3 "ambient0"JZ% +4 "core" %U | +2 #"sampling"Cartridge 51 filtering 277/306ml at 0.41ml/s with 27.9psi vacuum +1695 "Can"jXW.z2s\\S  +4293 "sampler0"H% +3 "core" ȅ%l. 2 +3 "ambient0"JJ% +5 "core" %  +2 #"sampling"Cartridge 51 filtering 300/306ml at 0.39ml/s with 22.2psi vacuum +1568 "core" ȴ' +2 "sampler0"H|% +5 "core" %,͕ 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."closeIntake"Intake.close +4 "sampler0"H3/ +6 ."closeExhaust"Exhaust.close +6 "sampler0"HC0 +7 "carousel0"FP% +10 "core" %0כN+ \ +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"D^( +102 "core" ȳ/ +21 0 +233 ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +9 ."FILTERING"Toroid.seek :clear +3 "valve0"D_( +355 "core" ( +3 #"FILTERING"Sampled 306.1ml +6 "carousel0"F(% +5 "core" ƨ%0כM% \ +8 "valve0"Da% +4 "core" %ZGROA \ +3 "syringe1"C\\& +5 "core" &'iU +2 "syringe1"Cd% +4 "core" %69v p +2 "carousel0"FK% +4 "core" %0ל@M \ +4 "carousel0"F@% +5 "core" %0כN* \ +4 "carousel0"Fh% +4 "core" %0כL \ +5 ."PROCESSING"Plunge.seek :pTop +2 "valve0"D`': +91 "core" ' +2 #"PROCESSING"Sample loop closed. Ready to Deliver Reagents +6 .Air.in :default +3 "syringe1"C>d P]z<2 +8 ."PROCESSING"Air.seek :sealed +3 "syringe1"CG' +137 ."sourcePumpShutdown"Gate.power :sampler,:OFF +135 "core" ' +3 ."PROCESSING"Air.in :slow +2 "syringe1"C>d P]z<2 +9 ."PROCESSING"Air.seek :arEvac +2 "syringe1"C['< +4804 "Can"iX.z1s= +2651 "core" ' +2 ."PROCESSING"delay 1:00 +6064 .Air.in :default +8 "syringe1"C>d P]z<2 +8 ."PROCESSING"Air.seek :clear +3 "syringe1"CY' +1868 "core" ' +3 #"PROCESSING"RNAlater soak for 10:00 +1348 "Can"lX*.z3tPk+2 +11958 lX .z2tNlP +11959 kX/.ztNǫn +11958 jX'.z(tMpj +11961 kX@.ztNv* +10820 ."PROCESSING"Air.seek :tVent +4 "syringe1"CQ'6 +247 "core" ' +5 ."PROCESSING"delay 1 second +104 .Air.in :halfSpeed +2 "syringe1"C>d P]z<2 +9 ."PROCESSING"Air.seek :arEmpty +3 "syringe1"Cl'< +765 "Can"lW.zs;A +2505 "core" ' +2 ."PROCESSING"delay 10 seconds +1004 .delay 1:00 +6005 .Plunge.seek :pTop +5 "valve0"Dq': +9 "core" ' +3 #"PROCESSING"Sample preserved and evacuated + "carousel0"Fs% +9 "core" %0ך \ +7 #"detached from nadia#3"Selecting Cartridge 40 +3 "carousel0"Fp% +8 "core" %0כ \ +3 "valve0"Dm% +4 "core" %:7|Om : +5 "valve1"Eg& +3 "core" &Om  +4 ."detached from nadia#3"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" ƙ%0כ \ +4 ."detached from nadia#3"Carousel.home.seek 51 +3 "carousel0"Fn'0 +18 "core" ' +2 "carousel0"Fi% +4 "core" %0Oכd  +4 "syringe0"B"& +5 "core" ¢&j*I +3 "syringe1"C}% +4 "core" %<.+~d +5 ."Air"Air.in :unlocking +3 "syringe0"B% +6 "syringe1"C> P]z-2 +7 "core" „%jپ6 +2 ."Air"Air.seek :clear +5 "syringe1"C' +466 "core" Ñ' +3 "syringe1"C & +5 "core" É&̷%D +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"D]% +2 "syringe1"C? P[(2 +10 ."Lysis1"Lysis1.seek :clear +4 "syringe1"Ct( +5 "core" %:7}Ob 9 +6 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +9 ."Plunge"Plunge.seek :clear +2 "valve0"DB'8 +107 "core" ( +35 ' +4 ."detached from nadia#3"Carousel.seek 40 +4 "carousel0"F='& +250 "core" ƽ' +5 "carousel0"FA% +3 "core" %&0 2 +3 ."detached from nadia#3"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +9 "syringe1"C2& +3 "core" ò&yvr +3 ."detached from nadia#3"Lysis1.in :fast +2 "syringe1"C?d P[<2 +9 ."detached from nadia#3"Lysis1.seek :locked +4 "syringe1"C\ ( +252 "core" Ê( +3 "valve0"D% +6 "core" Ă%8ZOA +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +3 "valve0"D'Z +11 "syringe1"Cc% +7 "core" %ݠaJ +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +8 ."Air"Air.seek :locked +3 "syringe1"C*'6 +63 "core" ā' +42 ê' +3 ."detached from nadia#3"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +8 F~% +3 "core" %&WD  +7 #"detached from nadia#3"Powering cartridge bus +265 "cartridge_broadcast" ( +2 ."detached from nadia#3"Gate.setIR 1750,5000,0,6 +15 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from nadia#3"PuckHeater.configure :default +3 "Cartridge" \ :D H  +8 ."detached from nadia#3"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" t +7 "cartridge" t +2 "Cartridge" y +5 "cartridge" y RI +3 "Cartridge" oy  +5 "cartridge" y k`/ C;R c/J59986 +3 #"detached from nadia#3"Mounted lysis Cartridge board#J59986 +8 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"D% +4 "core" ć%ZG;OM ! +3 "syringe1"Cu& +5 "core" &oP +2 "syringe1"Cr% +5 "core" %6hN +3 "carousel0"F/% +4 "core" Ư%&v \ +3 #"FILTERING"Preparing to filter microSPR_phyto cartridge in slot 40 +7 "valve0"D% +4 "core" ĕ%ZGWON  +3 "syringe1"C& +5 "core" Ø&eW +3 "syringe1"CH% +4 "core" %6oh +3 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +9 "core" ǔ&r +42 "carousel1"G& +3 "core" ǒ& +47 "carousel1"G& +3 "core" Lj& +47 "carousel1"Gv& +59 "core" &z +3 "carousel1"G& +4 "core" Ǔ&p +34 "carousel1"G& +3 "core" ǎ&fF +47 "carousel1"Gw& +3 "core" &}\\ +47 "carousel1"G& +3 "core" dž&o +47 "carousel1"G & +5 "core" Ǎ& +46 "carousel1"G& +3 "core" ǐ&c +26 "Can"mX5.ztNJ +21 "carousel1"G& +4 "core" ǝ&b +46 "carousel1"G& +3 "core" ǜ& +47 "carousel1"G-& +3 "core" ǭ&j +47 "carousel1"Gk& +4 "core" & +5 "Can"mX5.zt5U| + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 28C (drifted 1%) +10 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +11 .PhaseSeparator.in :filling + "carousel1"G?MM +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"GX, +5 "core" , +4 ."FILTERING"Gate.power :sampler,:ON +703 "core" ,Lk +7 #"FILTERING"Head pressure reached 3.38psi in 8 seconds +6 ."sealing"Air.in :default +4 "carousel0"F8% +8 "syringe1"C>d P]z<2 +2 "core" Ƹ%&L \ +3 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +9 ."sealing"Air.seek :sealed +6 ."FILTERING"SamplePump.setPosition! 0ml +6 "syringe1"C.' +3 "sampler0"H7 +8 ."FILTERING"Bypass.open +3 "sampler1"I1 +154 "core" ɞ1 +73 "ambient0"J1% +3 "core" ʱ% v +3 ."FILTERING"Intake.open +3 "sampler0"H1 +22 "core" î' +126 ț1 +2 "ambient0"J% +7 "core" ʃ% +2 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"Hj% +9 "core" % +42 "sampler0"H{% +4 "core" %6 +46 "sampler0"Hy% +3 "core" %3 +47 "sampler0"He% +3 "core" %0 +47 "sampler0"Hz% +3 "core" % +47 "sampler0"H)% +3 "core" ȩ%y +5 "Can"mX5.zs?WX( + #"FILTERING"Vacuum tare is 54% of full scale at 28C (drifted 2%) +2 "ambient0"JI% +10 "core" % +2 "ambient0"Jb% +4 "core" % +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +3 "ambient0"J + +7 "core" ʌ+ +2 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +8 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G', +5 "core" ǧ, +5 "sampler0"H% +7 "core" ȗ%w +2 "sampler0"H+% +4 "core" ȫ%x +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "18:17:48.94" +4497 "Can"mX5.zs>* +4 ."FILTERING"SamplePump.in :start +4 "sampler0"H>^ }n#(+@3 22Q +9 ."FILTERING"SamplePump.seek 10ml,1:10 +2 "sampler0"H!'6 +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(+@3 22Q +1289 "core" ȡ' +2 "sampler0"H% +4 "core" ȟ%6 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"SamplePump.setPosition! 0ml + "sampler0"H7 +7 ."FILTERING"Exhaust.open +2 "sampler0"H2 +154 "core" Ț2 +75 ."closeBypass"Bypass.close +4 "sampler1"I0/ +153 "core" ɰ/ +73 "sampler0"H:% +4 "core" Ⱥ%dn +4 "ambient0"J5% +4 "core" ʵ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"J&+ +7 "core" ʦ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G%, +9 "core" ǥ, +2 "ambient0"JT% +5 "core" % +3 "sampler0"HF% +4 "core" %[ +3 "sampler0"HE% +5 "core" %n +8 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n#(+@3 22Q +10 ."FILTERING"SamplePump.seek 200ml,13:50 +3 "sampler0"H7'G +54 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(+@3 22Q +3722 "Can"lX.z&sPWF +11956 lW.z/sRV*" +10546 "core" ȷ' +2 "sampler0"H?% +4 "core" ȿ%G 2 +62 ."FILTERING"Abs.stop +6 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."FILTERING"Bypass.open +6 "sampler1"I;1 +151 "core" ɻ1 +2 ."FILTERING"Intake.close +4 "sampler0"HD/ +146 "core" / +2 "carousel0"F#% +6 "core" ƣ%&J \ +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :crack +5 "valve0"DM( +169 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"DO(m +210 "core" ( +5 ."FILTERING"Toroid.in :default +3 "valve0"D? ֜2 +11 ."FILTERING"Toroid.seek :clear +3 "valve0"DS( +374 "core" ( +2 "ambient0"JL% +5 "core" %i +5 "Can"lW.z/soI ? +4 "ambient0"JR% +4 "core" %j +2 "ambient0"Jf% +4 "core" % +3 #"FILTERING"Pressurizing cartridge to 14psi above 14.6psia +3 .SamplePump.setPosition! 0ml +5 "sampler0"H7 +6 ."FILTERING"Intake.open +3 "sampler0"H<1 +77 "Can"kW.zsDe@ +68 "core" ȼ1 +3 ."FILTERING"Bypass.close +2 "sampler1"I$/ +150 "core" ɤ/ +2 ."FILTERING"Exhaust.close +2 "sampler0"H 0 +159 "core" ȋ0 +2 "sampler0"HZ% +4 "core" % +3 "ambient0"J% +4 "core" ʅ% +3 ."FILTERING"Abs.in :default + "ambient0"J>9 e  +10 ."FILTERING"Abs.hold +2 "ambient0"JJ+ +6 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G|, +9 "core" , +2 "sampler0"HP% +4 "core" % +4 "sampler0"H3% +4 "core" ȳ% +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>nu0\\3 22Q +8 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +6 "sampler0"HC' +1240 "core" ' 2 +4 "sampler0"H% +4 "core" ȏ%> 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +9 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."FILTERING"Intake.close +2 "sampler0"H6/ +151 "core" ȶ/ +2 "ambient0"Jx% +6 "core" % +2 #"FILTERING"Pressurized cartridge with 0.650ml to 14.1psi +2 #Isolated @27.8psia (13.2psi) +6 #Verifying cartridge holds at least 0.65..0.85 of 27.8psia +7 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +7 "ambient0"J^% +9 "core" % +41 "ambient0"J(% +5 "core" ʨ% +45 "ambient0"Ja% +5 "core" % +45 "ambient0"J\\% +4 "core" %z +47 "ambient0"Jd% +3 "core" % +46 "ambient0"JK% +4 "core" % +46 "ambient0"J@% +5 "core" %w +45 "ambient0"Jh% +5 "core" %% +45 "ambient0"JW% +4 "core" % +46 "ambient0"J,% +4 "core" ʬ% +47 "ambient0"JG% +3 "core" % +47 "ambient0"J[% +3 "core" % +47 "ambient0"JY% +3 "core" %z +47 "ambient0"JQ% +3 "core" %} +47 "ambient0"Js% +3 "core" %} +3 #"FILTERING"Cartridge pressure fell 13% to 24.3psia +2 "carousel0"Fp% +10 "core" %&H \ +3 #"FILTERING"Sampling 100.0ml +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +9 ."FILTERING"Exhaust.open +2 "sampler0"Hm2 +152 "core" 2 +3 ."FILTERING"Intake.open +3 "sampler0"Hg1 +151 "core" 1 +5 "Can"kW.zskKQG +2 "ambient0"J% +4 "core" ʙ%r +4 "ambient0"Ji% +4 "core" %z +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +8 ."FILTERING"Abs.hold +2 "ambient0"J"+ +66 "core" ʢ+ +3 ."FILTERING"PhaseSeparator.hold +16 "carousel1"G}, +9 "core" , +2 "ambient0"J% +4 "core" ʄ%o +4 "sampler0"H`% +4 "core" %  +3 "sampler0"HU% +5 "core" %  +7 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n#(@@3 22Q +10 ."FILTERING"SamplePump.seek 100ml,6:04 +4 "sampler0"Hl'# +53 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d n#(@@3 22Q +226 H % +3 "core" ȉ% \ S 2 +4 "ambient0"J]% +3 "core" %N | +3 #"sampling"Cartridge 40 filtering 2/100ml at 0.59ml/s with 13.2psi vacuum +5993 "sampler0"HV% +3 "core" %*   2 +3 "ambient0"Jq% +4 "core" %5 } +2 #"sampling"Cartridge 40 filtering 39/100ml at 0.63ml/s with 12.6psi vacuum +2507 "Can"kXQ.z%sTU^ +3481 "sampler0"HA% +3 "core" %Y 2 +3 "ambient0"J2% +4 "core" ʲ% } +2 #"sampling"Cartridge 40 filtering 73/100ml at 0.56ml/s with 17.9psi vacuum +5166 "core" ' +2 "sampler0"Hn% +4 "core" %#) 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +11 ."closeIntake"Intake.close +4 ."closeExhaust"Exhaust.close +9 "sampler0"H/ +3 "carousel0"Fc% +12 "sampler0"Ht0 +4 "core" %&THO \  +7 ."FILTERING"Toroid.in :hold +4 "valve0"D? ֜2 +11 ."FILTERING"Toroid.seek :bypass +3 "valve0"D~( +104 "core" Ȃ/ +22 0 +230 ( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +10 ."FILTERING"Toroid.seek :clear +6 "valve0"DB( +354 "core" ( +3 #"FILTERING"Sampled 100.0ml +3 "carousel0"F% +10 "core" Ƒ%&G \ +7 "valve0"D% +5 "core" %ZGFOL  +2 "syringe1"C & +4 "core" à&!Hn +3 "syringe1"Co% +4 "core" %K} +3 "carousel0"F% +4 "core" Ƈ%&H \  +6 "carousel0"Fu% +8 "core" %&H \  +121 ."PROCESSING"SPR.command "read_sequences" +28 .SPR.command "stats_on" +10 "carousel0"Fr% +3 "core" %&I \  +6 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3118h.spr.bz2 +20 ."sourcePumpShutdown"Gate.power :sampler,:OFF +176 ."PROCESSING"Handoff.select :waste +3 "valve0"D +53 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C/% +8 "core" ï%IP +42 "syringe1"C% +4 "core" Õ%FV +45 "syringe1"C% +4 "core" Ø%HX +46 "syringe1"CH% +4 "core" %F` +46 "syringe1"C% +5 "core" Ô%Ee +45 "syringe1"C% +4 "core" Ò%ف +47 "syringe1"C% +4 "core" È%Fm +45 "syringe1"Cv% +4 "core" %Du +47 "syringe1"C% +3 "core" Ó%Gf +47 "syringe1"C% +3 "core" Î%Bn +47 "syringe1"Cw% +3 "core" %Dq +47 "syringe1"C% +4 "core" Æ%@r +46 "syringe1"C % +3 "core" Í%n +48 "syringe1"C% +6 "core" Ð%m +6 "Can"kXQ.z%t(I$x +2 #"PROCESSING"Syringe tare is 51% of full scale at 28C (drifted -0%) +6 .PinPort.in :locking +3 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +4 "valve1"E( N +81 "core" ŝ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +11 ."posLyS2"Lysis2.in :fast +5 ."PROCESSING"Lysis1.seek 1250uL +7 ."posArchive"Archive.seek :empty +6 "syringe0"B>d P\\k<2 +4 "syringe1"C( +76 "syringe0"B-( +8 ."posLyS2"Lysis2.seek 950uL +13 "syringe0"Bk' +234 "core" Ü( +273 ' +313 ­( +2 "carousel0"F8% +5 "core" Ƹ%& \ +4 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +4 .PinPort.seek :locked +5 "valve1"E( N +10 "core" Ş( + ."PROCESSING"Handoff.select :waste +4 .Plunge.seek :puckBottom +5 "valve0"D1'B +128 "core" ı' +4 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +5 "syringe1"C*% +10 "core" ê%t +8 "Can"jW.zt?| +32 "syringe1"C% +4 "core" Û%z +46 "syringe1"C% +3 "core" Ã%} +47 "syringe1"Cj% +4 "core" %t +46 "syringe1"C{% +3 "core" %u +47 "syringe1"Cy% +3 "core" % +47 "syringe1"Ce% +4 "core" %p +46 "syringe1"Cz% +5 "core" %n +46 "syringe1"C)% +3 "core" é%o +49 "syringe1"CI% +5 "core" %m +43 "syringe1"Cb% +3 "core" %r +47 "syringe1"C% +3 "core" ×%m +47 "syringe1"C+% +3 "core" ë%t +47 "syringe1"CX% +3 "core" %! +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi +2 #Air stroke #1 of 1 +5 "syringe1"C4% +8 "core" ô%r +3 ."PROCESSING"Air.in :pressurize +3 "syringe1"C>@N Q P]z<2 +7 ."PROCESSING"Air.seek :pressurized +5 "syringe1"C' +517 "core" ß' +3 "syringe1"C% +8 "core" Ú%#S < +2 #"PROCESSING"0.3psi after pressurize +3 .Air.in :compress +7 "syringe1"C>N Q P]zP2 +6 ."PROCESSING"Air.seek :evac +3 "syringe1"C '| +299 "core" Ì'pE kP +4 "syringe1"C'% +4 "core" ç%#Ew sP +3 #"PROCESSING"Reached 0.0 of 4.0psi after pumping 1572uL (0.89 strokes) +3 .Air.in :default +6 "syringe1"C>d P]z<2 +7 ."PROCESSING"Air.hold +2 "syringe1"C0+ +7 "core" ð+ +4 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C:% +14 "core" ú%Ecr +36 "syringe1"C5% +3 "core" õ%E^ +49 "syringe1"CT% +5 "core" %Eg +43 "syringe1"CF% +5 "core" %E^ +45 "syringe1"CE% +4 "core" %Ea +46 "syringe1"C!% +3 "core" á%E\\ +47 "syringe1"C?% +3 "core" ÿ%Ec +47 "syringe1"C;% +3 "core" û%Ed +48 "syringe1"C&% +4 "core" æ%Eax +46 "syringe1"C%% +3 "core" å%Ecj +47 "syringe1"CD% +3 "core" %E`q +47 "syringe1"C#% +3 "core" ã%EZ\\ +47 "syringe1"C_% +3 "core" %EaH +47 "syringe1"CM% +4 "core" %E^I +46 "syringe1"CO% +3 "core" %E`: +47 "syringe1"CL% +3 "core" %E`7 +47 "syringe1"CR% +3 "core" %EXL +47 "syringe1"Cf% +60 "core" %ESG +4 "syringe1"C<% +5 "core" ü%E\\Q +32 "syringe1"C$% +5 "core" ä%E]D +44 "syringe1"C % +3 "core" Ë%E]M +47 "syringe1"CZ% +3 "core" %EV\\ +47 "syringe1"C% +3 "core" Å%E]j +3 #"PROCESSING"Stabilized at -0.1psi after evac to waste + .Handoff.select :blocked +9 "valve0"D0 +48 ."PROCESSING"Plunge.seek :puckTop +4 "valve0"DP': +87 "core" ' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi +2 #Air stroke #1 of 8 +2 "syringe1"C3% +12 "core" ó%EW- +4 ."PROCESSING"Air.in :compress + "syringe1"C>N  P]zP2 +9 ."PROCESSING"Air.seek :evac +4 "syringe1"C7'| +366 "core" ÷' +3 "syringe1"C% +3 "core" Ï%|4Ņ oPo +4 #"PROCESSING"5.9psi after compress +3 "syringe1"C6% +11 "core" ö%|49 h +39 "syringe1"Cx% +3 "core" %|4 W +47 "syringe1"C^% +4 "core" %|4 h +46 "syringe1"C(% +4 "core" è%|4b n +46 "syringe1"Ca% +4 "core" %|4 c +46 "syringe1"CJ% +4 "core" %|4 s +46 "syringe1"C|% +4 "core" %|4 ^ +46 "syringe1"C\\% +4 "core" %|4 i +46 "syringe1"Cd% +4 "core" %|4 l +46 "syringe1"CK% +4 "core" %|4 c +46 "syringe1"C@% +5 "core" %|43 a +45 "syringe1"Ch% +4 "core" %|4k* f +47 "syringe1"CW% +6 "core" %|41 c +43 "syringe1"C,% +5 "core" ì%|4* ` +47 "syringe1"CG% +3 "core" %|4/ a +45 "syringe1"C[% +4 "core" %|40 Z +47 "syringe1"CY% +4 "core" %|4- f +46 "syringe1"CQ% +3 "core" %|4: ^ +47 "syringe1"Cs% +3 "core" %|4C] s +47 "syringe1"Cp% +3 "core" %|4ec g +47 "syringe1"Cm% +4 "core" %|4q h +46 "syringe1"Cg% +3 "core" %|4 a +47 "syringe1"C% +3 "core" Ù%|4{ a +47 "syringe1"Ci% +4 "core" %|4 h +46 "syringe1"C% +3 "core" Ä%|4 d +3 #"PROCESSING"9.7psi after compress (stable) +6 .Air.in :gulp +3 "syringe1"C>N  P]z<2 +8 ."PROCESSING"Air.seek :topVent +5 "syringe1"C`'6 +637 "core" ' +2 "syringe1"CU% +4 "core" %6;ɔ- < +2 #"PROCESSING"9.4psi after gulp +3 "syringe1"C % +9 "core" É%6ɟ7 +43 "syringe1"C]% +6 "core" %6ɢ> +42 "syringe1"CC% +4 "core" %6ɟ5 +46 "syringe1"CV% +4 "core" %6ɣ( +46 "syringe1"Cq% +3 "core" %6ɠ5 +47 "syringe1"CA% +4 "core" %6ɡ) +47 "syringe1"C2% +3 "core" ò%6ɜ# +47 "syringe1"Cn% +3 "core" %6ɦ~) +58 "syringe1"Cc% +4 "core" %6ɡ/ +35 "syringe1"C% +3 "core" Â%6ɝ~B +47 "syringe1"Ct% +4 "core" %6ɣ; +46 "syringe1"C"% +4 "core" â%6ɞ> +46 "syringe1"C}% +3 "core" %6ɚ8 +3 #"PROCESSING"9.4psi after gulp (stable) + #Air stroke #2 of 8 +4 "syringe1"CS% +13 "core" %6ɚ: +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +9 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C~' +417 "core" 'U} d P]z<2 +10 ."PROCESSING"Air.hold +6 "syringe1"C+ +7 "core" + +3 ."PROCESSING"Plunge.seek :puckBottom +4 "valve0"D 'B +85 "core" Ġ' +2 ."PROCESSING"delay 3 seconds +303 .Handoff.select :waste +2 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Co% +9 "core" %}v* T +42 "syringe1"C% +3 "core" Ç%}xi T +49 "syringe1"Cu% +5 "core" %}v% T +43 "syringe1"Cr% +3 "core" %}w L +47 "syringe1"C/% +3 "core" ï%}t O +47 "syringe1"C% +3 "core" Õ%}tk U +47 "syringe1"C% +3 "core" Ø%}r S +47 "syringe1"CH% +3 "core" %}o L +48 "syringe1"C% +3 "core" Ô%}n M +47 "syringe1"C% +3 "core" Ò%}s U +47 "syringe1"C% +6 "core" È%}s S +44 "syringe1"Cv% +4 "core" %}q O +46 "syringe1"C% +3 "core" Ó%}p U +47 "syringe1"C% +3 "core" Î%}p [ +47 "syringe1"Cw% +5 "core" %}l K +45 "syringe1"C% +3 "core" Æ%}sx V +3 ."PROCESSING"delay 1:00 +4525 "Can"mW.z.t@# +1477 ."PROCESSING"Handoff.select :blocked +5 "valve0"D0 +56 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi +3 "syringe1"C % +8 "core" Í%}Zd T +42 "syringe1"C% +4 "core" Ð%}[h N +46 "syringe1"C9% +3 "core" ù%}[j T +47 "syringe1"C\ % +5 "core" Ê%}Yc S +45 "syringe1"C>% +3 "core" þ%}%~ R +47 "syringe1"CN% +3 "core" %}[j V +47 "syringe1"C8% +5 "core" ø%}Wg M +45 "syringe1"C*% +3 "core" ê%}Wh N +47 "syringe1"C% +3 "core" Û%}Ue O +47 "syringe1"C% +4 "core" Ã%}T_ S +46 "syringe1"C% +3 "core" Ý%}Vg O +47 "syringe1"Cj% +3 "core" %}Rc R +47 "syringe1"C% +5 "core" Á%}Nb Q +45 "syringe1"C{% +7 "core" %}TT X +43 "syringe1"Cy% +3 "core" %}Lc O +48 "syringe1"Ce% +4 "core" %}RV P +45 "syringe1"Cz% +3 "core" %}Qj [ +47 "syringe1"C)% +3 "core" é%}K J +47 "syringe1"CI% +3 "core" %}L T +47 "syringe1"Cb% +4 "core" %}K Y +46 "syringe1"C% +5 "core" ×%} M +45 "syringe1"C+% +3 "core" ë%} P +47 "syringe1"CX% +3 "core" %} O +47 "syringe1"C4% +3 "core" ô%} Q +47 "syringe1"C.% +3 "core" î%} L +47 "syringe1"C% +3 "core" Ú%}} V +47 "syringe1"C% +3 "core" ß%}v H +47 "syringe1"C'% +3 "core" ç%}i S +47 "syringe1"C:% +3 "core" ú%}j O +47 "syringe1"C5% +4 "core" õ%}a S +46 "syringe1"CT% +4 "core" %}j X +46 "syringe1"CF% +4 "core" %}s T +48 "syringe1"CE% +4 "core" %} S +44 "syringe1"C!% +3 "core" á%} X +47 "syringe1"C % +3 "core" Ì%} T +47 "syringe1"C?% +3 "core" ÿ%} Y +47 "syringe1"C;% +3 "core" û%} X +47 "syringe1"C&% +3 "core" æ%}G Z +47 "syringe1"C%% +3 "core" å%} X +47 "syringe1"CD% +4 "core" %} Q +47 "syringe1"C#% +3 "core" ã%} Q +47 "syringe1"C_% +3 "core" %}~ M +47 "syringe1"CM% +3 "core" %} T +47 "syringe1"CO% +3 "core" %}~ U +47 "syringe1"CL% +3 "core" %} Q +47 "syringe1"CR% +3 "core" %}~ W +3 ."PROCESSING"Handoff.select :waste +8 "valve0"D +49 #"PROCESSING"0.1psi at evacEnd +5 .Plunge.seek :pTop +69 "valve0"Df': +85 "core" ' +3 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +2 "syringe1"C>d  P]z<2 +8 ."PROCESSING"Air.seek :fullVent +4 "syringe1"C<'݇ +932 "core" ü' +2 ."PROCESSING"delay 2 seconds +204 .Plunge.seek :pBottom +2 "valve0"D$'B +85 "core" Ĥ' + #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +3 .Plunge.seek :locked +10 "valve0"D 'Z +126 "core" ċ' +2 ."PROCESSING"Handoff.select :waste +3 "syringe1"CZ& +9 "core" &pd ? +3 ."PROCESSING"Lysis1.in :slow +2 "syringe1"C?d P[<2 +7 ."PROCESSING"Lysis1.seek 950uL +3 "syringe1"C( +1407 "core" Å( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select :blocked +3 "valve0"D0 +52 ."PROCESSING"Plunge.seek :puckTop +6 "valve0"D3': +86 "core" ij' +3 "syringe1"C1& +4 "core" ñ&zMg n +3 ."PROCESSING"Lysis1.seek 350uL +4 "syringe1"C0( +2801 "core" ð( +4 ."PROCESSING"delay 3 seconds +303 "syringe1"C% +3 "core" Ï%݇/om +4 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 +2 "syringe1"C> wk+ P]z<2 +10 C6% +3 "core" ö%݇/3u +4 "syringe1"Cx& +5 "core" &Giy  +69 ."Airmixer"Air.seek :evac +6 "syringe1"C^'| +10 ."PROCESSING"Lysis1.seek 190uL +8 "syringe1"C(( +722 "Can"jX7.z s/_N v +27 "core" ' +8 è( +3 ."PROCESSING"Air.in :fast +2 "syringe1"C> P]z<2 +9 ."PROCESSING"Air.seek :full +4 "syringe1"Ca'6 +649 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"CJ( +897 "core" ( +2 "valve0"D|% +4 "core" %:7Ol 3 +3 "syringe1"C\\% +4 "core" %68ɦ΋ +3 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @14.5psi +3 .Plunge.seek :puckBottom +7 "valve0"Dd'B +85 "core" ' +2 "valve0"DK% +4 "core" %Bw(!X~ - +2 "syringe1"C@% +5 "core" %6ɪT +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @11.0psi +2 #Polling Can every 2:00 +56 "Can"kW.ztN +4 ."PROCESSING"PuckHeater.configure :default #ambient 28.2C +3 "Cartridge" \ :D H  +6 ."PROCESSING"PuckHeater.seek 81.3C +6 "Cartridge" h : +3 W9 +6 "cartridge" 9' +104 "Cartridge" ,9 +3 "cartridge" 9'Gd +103 "Cartridge" G9 +4 "cartridge" 9(d +103 "Cartridge" [9 +5 "cartridge" 9*m d +103 "Cartridge" Y9 +3 "cartridge" 9- d +105 "Cartridge" Q9 +4 "cartridge" 911d +103 "Cartridge" s9 +3 "cartridge" 94hd +104 "Cartridge" p9 +3 "cartridge" 97d +104 "Cartridge" m9 +5 "cartridge" 9:Vd +103 "Cartridge" g9 +4 "cartridge" 9<#d +103 "Cartridge" 9 +3 "cartridge" 9?J'd +104 "Cartridge" i9 +3 "cartridge" 9Ay+d +104 "Cartridge" 9 +6 "cartridge" 9Ce0d +104 "Cartridge" 79 +3 "cartridge" 9E54d +104 "Cartridge" U9 +3 "cartridge" 9G8d +103 "Cartridge" 9 +3 "cartridge" 9H=d +104 "Cartridge" ]9 +3 "cartridge" 9J_HAd +104 "Cartridge" C9 +3 "cartridge" 9KEd +104 "Cartridge" V9 +4 "cartridge" 9MYJd +103 "Cartridge" q9 +4 "cartridge" 9NHNd +104 "Cartridge" A9 +3 "cartridge" 9P9HRd +103 "Cartridge" 29 +7 "cartridge" 9QHWd +104 "Cartridge" n9 +4 "cartridge" 9Rt[d +104 "Cartridge" c9 +3 "cartridge" 9Tat_d +103 "Cartridge" 9 +62 "cartridge" 9V1tfd +104 "Cartridge" t9 +4 "cartridge" 9Wmjd +103 "Cartridge" "9 +6 "cartridge" 9Xnd +105 "Cartridge" }9 +3 "cartridge" 9YHsd +103 "Cartridge" S9 +4 "cartridge" 9[<#wd +104 "Cartridge" `9 +3 "cartridge" 9\\C{d +105 "Cartridge" 9 +3 "cartridge" 9]#d +104 "Cartridge" ~9 +3 "cartridge" 9^d +103 "Cartridge" P9 +4 "cartridge" 9_d +103 "Cartridge" o9 +4 "cartridge" 9`d +104 "Cartridge" 9 +3 "cartridge" 9ad +103 "Cartridge" u9 +4 "cartridge" 9bNd +103 "Cartridge" r9 +3 "cartridge" 9cd +106 "Cartridge" /9 +6 "cartridge" 9dd +105 "Cartridge" 9 +4 "cartridge" 9ed +103 "Cartridge" 9 +6 "cartridge" 9fd +105 "Cartridge" H9 +4 "cartridge" 9gNd +103 "Cartridge" 9 +4 "cartridge" 9h#d +103 "Cartridge" 9 +3 "cartridge" 9i#d +104 "Cartridge" 9 +3 "cartridge" 9j#d +106 "Cartridge" v9 +5 "cartridge" 9kzd +104 "Cartridge" 9 +4 "cartridge" 9l#d +103 "Cartridge" 9 +3 "cartridge" 9mzd +103 "Cartridge" w9 +4 "cartridge" 9nc +103 "Cartridge" 9 +5 "cartridge" 9oqd +103 "Cartridge" 9 +3 "cartridge" 9pNzd +104 "Cartridge" 9 +3 "cartridge" 9q"zc +104 "Cartridge" 99 +7 "cartridge" 9rzd +105 "Cartridge" \ 9 +6 "cartridge" 9s\ d +104 "Cartridge" >9 +4 "cartridge" 9sd +103 "Cartridge" N9 +4 "cartridge" 9tb +103 "Cartridge" 89 +3 "cartridge" 9ud +104 "Cartridge" *9 +3 "cartridge" 9vqd +104 "Cartridge" 9 +3 "cartridge" 9wKd +103 "Cartridge" 9 +5 "cartridge" 9x!d +105 "Cartridge" 9 +3 "cartridge" 9xd +104 "Cartridge" j9 +4 "cartridge" 9yd +103 "Cartridge" 9 +3 "cartridge" 9z)d +104 "Cartridge" {9 +4 "cartridge" 9{r\ c +103 "Cartridge" y9 +5 "cartridge" 9|L)d +103 "Cartridge" e9 +3 "cartridge" 9})d +104 "Cartridge" z9 +7 "cartridge" 9})c +163 "Cartridge" )9 +3 "cartridge" 9CUd +103 "Cartridge" I9 +4 "cartridge" 9U"c +104 "Cartridge" b9 +6 "cartridge" 9U'c +104 "Cartridge" 9 +4 "cartridge" 9+d +103 "Cartridge" +9 +7 "cartridge" 9CU/c +104 "Cartridge" X9 +3 "cartridge" 94b +104 "Cartridge" 49 +3 "cartridge" 98a +104 "Cartridge" .9 +3 "cartridge" 9rU<_ +103 "Cartridge" 9 +4 "cartridge" 9+@\\ +4 ."PROCESSING"PuckHeater.hold 2:00,81.3C +12 "Cartridge"  :Ƌ +7 "cartridge" A +2031 "Can"kWt.z&s9 +4 kWt.z&s:7\ +9923 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +52 "Can"lW.z'tM +3 ."PROCESSING"PuckHeater.configure :default #ambient 28.3C +6 "Cartridge" \ :D H  +5 ."PROCESSING"PuckHeater.seek 80.3C +6 "Cartridge" :9 +4 ' b +4 "cartridge" 9 +8 ."PROCESSING"PuckHeater.hold 2:00,80.3C +10 "Cartridge" 5 b +5 "cartridge"  +1926 "Can"lW.z1t 'X +3 lW.z1s(GY +10035 "cartridge" +4 #"PROCESSING"Polling Can every 2:00 +53 "Can"iW.z!tND= ] +2 ."PROCESSING"PuckHeater.configure :default #ambient 28.3C +3 "Cartridge" \ :D H  +9 ."PROCESSING"PuckHeater.hold 8:00,79.2C +9 "Cartridge" T  +1838 "Can"kW.z1t%0  +4 kW.z1t\ %  +11953 kW.z1t%F$J 0 +3 kW.z1t%$J 0\ +11951 mW.zt%6( N +3 mW.zt%J( N\ +11952 kW.z,t%X- l +3 kW.z,t%|- l\ +10133 "cartridge" +3 "syringe1"CF% +3 "core" %6ɡT' +2 #"PROCESSING"Lysate cooked @6.5psi +3 .Plunge.seek :puckTop +9 "valve0"DE': +86 "core" ' +3 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi + #Air stroke #1 of 5 +7 "syringe1"C!% +12 "core" á%6ɥQG +2 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N > P]z<2 +11 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C ' +630 "core" Ì' +2 "syringe1"C?% +8 "core" ÿ%(S < +2 #"PROCESSING"6.3psi after pressurize +8 .Air.in :compress +5 "syringe1"C>N > P]zP2 +8 ."PROCESSING"Air.seek :evac +6 "syringe1"C;'| +608 "core" û'p6žO kP +4 "syringe1"C&% +4 "core" æ%6. rP +2 #"PROCESSING"Reached 41.3 of 40.0psi after pumping 1749uL (0.99 strokes) +7 .Air.in :default +7 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.hold +3 "syringe1"C%+ +7 "core" å+ +4 ."PROCESSING"delay 2 seconds +203 .Air.in :fast +2 "syringe1"C> P]z<2 +8 ."PROCESSING"Air.seek :full +3 "syringe1"CD'6 +133 "Can"mW.zs>=1  +506 "core" ' +61 ."PROCESSING"Plunge.seek :puckBottom +21 "valve0"D#'B +84 "core" ģ' +2 "syringe1"C_& +5 "core" &*U  +3 ."PROCESSING"Lysis1.seek 138uL +3 "syringe1"CM(# +655 "core" ( +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :puckTop +3 "valve0"DO': +86 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +5 "syringe1"CL( +653 "core" ( +3 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"DR'B +87 "core" ' +2 "carousel0"F % +5 "core" Ơ%& \ +4 "valve0"D% +5 "core" %Bv(Xj - +4 "syringe1"CZ% +6 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d '=J P[#2 +8 "core" %6ɴ}} +4 ."PROCESSING"Lysis1.seek 675uL,nil +3 "syringe1"Cf( +9 C$& +3 "core" ä&0*Yt| # +4 "syringe1"C& +6 "core" Ü&*jt # +7 "syringe1"C1& +3 "core" ñ& * # +12 "syringe1"C & +4 "core" Ë&/!,"( # +4 "syringe1"C% +5 "core" Å%6!ɮ{ +7 "syringe1"C& +4 "core" Ï&ݐ  .3 # +16 "syringe1"C6& +4 "core" ö& 0W # +16 "syringe1"Cx& +4 "core" &U  2 # +4 "syringe1"C<% +4 "core" ü%6#ɶ$ +9 "syringe1"C0& +3 "core" ð&޺ "4: # +16 "syringe1"C^& +7 "core" & #6 # +13 "syringe1"C(& +4 "core" è&z  7 # +4 "syringe1"C|% +4 "core" %6ɼo +8 "syringe1"C\\& +4 "core" & 9 # +16 "syringe1"CK& +4 "core" &= ; # +16 "syringe1"C@& +4 "core" & = # +3 "syringe1"CW% +5 "core" %6 ɲu +8 "syringe1"C,& +4 "core" ì& ? # +16 "syringe1"C3& +4 "core" ó&c AAL # +16 "syringe1"CG& +4 "core" & C*t # +3 "syringe1"C[% +4 "core" %6ɼ8 +9 "syringe1"CY& +4 "core" && E # +16 "syringe1"CQ& +3 "core" & F) #! +17 "syringe1"Cs& +8 "core" &(I #, +2 "syringe1"Cp% +7 "core" %6ɽG +2 "syringe1"Cm% +4 "core" %6ɫ +4 ."pump"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +8 Cg& +3 ."pump"Air.seek :pressurized +7 "syringe1"C' +8 "core" &Luty #& +4 "syringe1"Ci& +9 "core" &A6NC # +3 "syringe1"C& +6 "core" Ä&@N # +4 "syringe1"C7& +3 "core" ÷&0dJO\\ # +17 "syringe1"CU& +3 "core" &PLO # +16 "syringe1"C & +4 "core" É&V=P%I # +16 "syringe1"C]& +4 "core" &V(P +16 "syringe1"CC& +4 "core" &V0P  +16 "syringe1"CV& +5 "core" &V7P  +15 "syringe1"Cq& +7 "core" &V4P  +13 "syringe1"CA& +4 "core" &V:P!  +16 "syringe1"C2& +3 "core" ò&V3P +17 "syringe1"Cn& +3 "core" &V4P +17 "syringe1"Cc& +3 "core" &V=P  +17 "syringe1"C& +4 "core" Â&V?P  +16 "syringe1"Ct& +4 "core" &VAP!  +16 "syringe1"C"& +5 "core" â&VGP \ +15 "syringe1"C}& +4 "core" &VdN  P]z<2 +7 ."pump"Air.seek :evac +3 "syringe1"C'| +8 Cv& +3 "core" &VP T  +4 "syringe1"C& +9 "core" Ó&VFP* +6 "syringe1"C& +3 "core" Î&V+P  +17 "syringe1"Cw& +4 "core" &VRP( +16 "syringe1"C& +5 "core" Æ&VIP  +15 "syringe1"C & +3 "core" Í&VGP  +19 "syringe1"C& +3 "core" Ð&VNP  +15 "syringe1"C9& +3 "core" ù&VSP +17 "syringe1"C\ & +3 "core" Ê&V,P \ +17 "syringe1"C>& +3 "core" þ&V;P  +17 "syringe1"CN& +3 "core" &VFP   +17 "syringe1"C8& +3 "core" ø&V.P +17 "syringe1"C*& +3 "core" ê&VSP  +17 "syringe1"C& +3 "core" Û&VUP +17 "syringe1"C& +3 "core" Ã&VWP +17 "syringe1"C& +6 "core" Ý&VGP  +14 "syringe1"Cj& +3 "core" &VVP  +17 "syringe1"C& +3 "core" Á&VSP   +17 "syringe1"C{& +3 "core" &VQP +17 "syringe1"Cy& +3 "core" &V>P  +17 "syringe1"Ce& +4 "core" &VOP +16 "syringe1"Cz& +4 "core" &VVP  +16 "syringe1"C)& +4 "core" é&VXP \ +16 "syringe1"CI& +3 "core" &V[P  +17 "syringe1"Cb& +3 "core" &VWP +18 "syringe1"C& +6 "core" ×&VEP  +13 "syringe1"C+& +3 "core" ë&VVP!=  +17 "syringe1"CX& +3 "core" &V[P7  +17 "syringe1"C4& +3 "core" ô&V4P8  +17 "syringe1"C.& +3 "core" î&VOPn +17 "syringe1"C& +3 "core" Ú&V[P!}  +17 "syringe1"C& +3 "core" ß&V_Pb  +17 "syringe1"C:& +3 "core" ú&VVP +17 "syringe1"C5& +3 "core" õ&VeP +17 "syringe1"Ch& +3 "core" &VcP  +17 "syringe1"CT& +3 "core" &VdP  +17 "syringe1"CF& +3 "core" &VDP +17 "syringe1"C!& +3 "core" á&V\\P!  +17 "syringe1"Cd& +3 "core" &VPP +19 "syringe1"Ca& +5 "core" &VIP   +13 "syringe1"C?& +3 "core" ÿ&V_P +17 "syringe1"C & +3 "core" Ì&VXP  +17 "syringe1"C&& +3 "core" æ&V_P7  +17 "syringe1"C;& +3 "core" û&V^P@ +18 "syringe1"C%& +3 "core" å&VfPp  +16 "syringe1"C_& +3 "core" &V6P\\  +17 "syringe1"CE& +4 "core" &VKP  +16 "syringe1"CJ& +4 "core" &V^P +16 "syringe1"C#& +3 "core" ã&VCP +17 "syringe1"CM& +3 "core" &VaP +17 "syringe1"C & +3 "core" à&VfP +17 "syringe1"C& +3 "core" &VkP  +17 "syringe1"CZ& +3 "core" &V_P +17 "syringe1"C$& +3 "core" ä&VmP +17 "syringe1"C& +3 "core" Ü&V(P!A # +17 "syringe1"C1& +3 "core" ñ&V&P܌ #\ +17 "syringe1"C & +3 "core" Ë&XLP#Ԍ8 # +17 "syringe1"C& +3 "core" Å&[xP+׌_ # +18 "syringe1"C& +4 "core" Ï&`1RPSҌ[ ~# +19 "syringe1"C6& +4 "core" ö&hJrPsیL # +15 "syringe1"Cx& +4 "core" &ra{PٌX # +13 "syringe1"C<& +3 "core" ü&}yPԌf }# +17 "syringe1"C0& +3 "core" ð&cQ.ЌQ # +17 "syringe1"C^& +3 "core" &\\Qь_ # +17 "syringe1"C(& +3 "core" è&wR،s ~# +17 "syringe1"CO& +3 "core" &YRҌZ }# +17 "syringe1"C|& +3 "core" &wS-Ҍk ~# +17 "syringe1"C\\& +3 "core" & SЌ? |# +17 "syringe1"CK& +3 "core" &*Tľ; }# +17 "syringe1"C@& +4 "core" &J|UҌ |# +16 "syringe1"CW& +3 "core" &j~Uь }# +17 "syringe1"C,& +3 "core" ì&Vkό }# +17 "syringe1"C3& +5 "core" ó&$W6ˌ # +15 "syringe1"CG& +3 "core" &6hW̋ {# +17 "syringe1"C[& +4 "core" &_|X֋ # +16 "syringe1"CL& +3 "core" &)jYˋ |# +71 "syringe1"CY& +3 "core" &G\\]ϊ # +4 "syringe1"CQ& +17 "core" &-z]Ί ~# +2 "syringe1"Cs& +4 "core" &{^ӊ |# +2 "syringe1"Cp& +4 "core" &#^ ~# +9 "syringe1"Cm& +4 "core" &0^ˊ ~# +16 "syringe1"Cg& +4 "core" &<)T_ˊ ~# +16 "syringe1"Ci& +4 "core" &=u_&/# {# +16 "syringe1"C& +4 "core" Ä&=W_'1 +16 "syringe1"C7& +4 "core" ÷&=L_)c +16 "syringe1"CU& +4 "core" &=m_#틙 +16 "syringe1"C & +4 "core" É&=n_)鋶 +16 "syringe1"C]& +4 "core" &=p_( +16 "syringe1"CC& +4 "core" &=o_) +16 "syringe1"CV& +4 "core" &=r_+ +16 "syringe1"Cq& +5 "core" &=w_'&= # +15 "syringe1"CA& +4 "core" &=v_+&L ~# +16 "syringe1"C2& +4 "core" ò&A/g_C' {# +9 È':\\=_! ~<| +6 "syringe1"Cn% +5 "core" %:==W < +2 #"pump"Reached 15.5 of 15.5psi after pumping 1671uL (0.94 strokes) +2 .Air.in :default +6 "syringe1"C>d P]z<2 +6 ."pump"Air.hold +5 "syringe1"Cc+ +9 C& +7 "core" + + Â&i` # +3 "syringe1"Ct% +6 C"& +6 "core" %:=Zό + â& `| # +2 "syringe1"CD& +7 "core" &,` # +4 "syringe1"C}& +3 "core" &>a3錔 # +10 "syringe1"CS& +3 "core" &]a| # +17 "syringe1"C`& +3 "core" &~bU # +5 "syringe1"C% +3 "core" Ñ%:=We +7 ( +4 "syringe1"C~& +5 "core" &ceo # +4 "syringe1"CP% +6 "core" %:=Sߌ +2 #"PROCESSING"Pulled 675uL lysate @15.4psi +3 "valve0"Do% +10 "core" %B(OV +4 "syringe1"C% +8 ."PROCESSING"Lysis1.seek 800uL,nil +6 "syringe1"Cu(% +5 "core" Ç%:=Z +3 "syringe1"Cr& +5 "core" &co挮 # +4 "syringe1"C/& +4 "core" ï&1cr # +8 "syringe1"C& +3 "core" Õ&a c܌ #} +17 "syringe1"C& +5 "core" Ø&(c茲 # +9 "syringe1"CH% +3 "core" %:=Y䌰 +6 "syringe1"C& +3 "core" Ô&Ddo茫 # +14 "syringe1"C& +3 "core" Ò&] d錌 # +17 "syringe1"Cv& +3 "core" &0ev # +7 "syringe1"C% +4 "core" Ó%:=][ ~ +3 "syringe1"C% +4 "core" Î%:=SB +3 ."pump"Air.in :compressLysate +2 "syringe1"C>dN  P]z<2 +9 ."pump"Air.seek :evac +4 "syringe1"Cw& +8 C'| +7 "core" &KgY- # +3 "syringe1"C & +6 "core" Í&^bg #w +4 "syringe1"C& +5 "core" Ð&rRhe #q +8 "syringe1"C9& +3 "core" ù&'hh! #u +17 "syringe1"C\ & +3 "core" Ê&[Njx ~#h +17 "syringe1"C>& +3 "core" þ&VkG_ #f +17 "syringe1"CN& +4 "core" &Rll! #U +16 "syringe1"C8& +3 "core" ø&$ln& ~#I + ( +3 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +9 ."pump"Air.hold +4 "syringe1"C*+ +8 "core" Æ'T;ދh d  P]z<2 +8 ."PROCESSING"Air.seek :fullVent +3 "syringe1"C'݇ +255 "core" Ý' +2 ."PROCESSING"Handoff.select :waste +3 "valve0"D +50 #"PROCESSING"Recovered 800uL lysate @13.6psi +2 .delay 3 seconds +306 "syringe1"C% +3 "core" Ù%݇/ +3 #"PROCESSING"After 3 delay Recovered 800uL lysate @0.1psi +3 .Plunge.seek :locked +6 "valve0"Dj'Z +131 "core" ' +2 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +10 B% +4 "core" %{\\ { +2 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +8 C{& +3 "core" &%nZ| U +78 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 550uL +7 "syringe0"By'| +5 "syringe1"Ce( +3489 "core" ' +4 ( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +7 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +118 #SPR stabilized +2 "syringe1"Cz& +7 "core" &X[+ +4 "syringe0"B)% +6 "core" ©%|e" +2 ."PROCESSING"delay 20 seconds +1014 "Can"kW.zt997  +988 ."PROCESSING"SPR.command "start 2 0" +260 #Waiting up to 11:00 for SPR to enter state 1 +10711 "Can"kW.z"t6<  +1057 ."PROCESSING"SPR.command "hold_on" +141 #SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +7 "syringe0"BI% +5 "core" %|ej+ +5 "syringe1"Cb& +4 "core" &Xm3 +72 ."Lysis2mixer"Lysis2.seek 400uL +6 ."PROCESSING"Lysis1.seek 250uL +6 "syringe0"B' +4 "syringe1"C+( +4188 "core" —' +2 ë( +5 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +254 #Waiting up to 1:00:00 for SPR to enter state 7 +4698 "Can"lW.zs.vD  +11960 jW.z#t4H\  +11960 kWr.z!t6L\  +11958 lW.zt2O\ > +11960 mW.zt5S\ \\ +11960 jW.zt8V\ z +11956 jWo.z)t4eZ2\  +11958 lW.zt2]\  +9148 #"SPRlogger"SPRsummary:-18.8RIU,182RIU,239RIU,309RIU,none,none,none after 2519s +2814 "Can"mW.zt$-a\\\  +11958 mW.zt#e\  +11958 mWO.z1t$4i  +11959 jWp.z/t/m . +1701 #"nadia"-> Head.psi +2 "carousel1"GX& +7 "core" & +353 #"nadia"-> Head.counts +3 "carousel1"G4& +9 "core" Ǵ& +6631 #"nadia"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +1084 #-> Head.psi +3 "carousel1"G.& +7 "core" Ǯ&N +2082 #"nadia"-> head.psi +4 !o:NoMethodError\ : mesgu:NameError::message,undefined method `psi' for :head:Symbol:@timestampu: Time F :: name:psi: args[:bt[""nadia:52:in `topLevelBinding'" Head.psi +5 "carousel1"G& +8 "core" ǚ&mdk +1186 #"nadia"-> Head.psi +5 "carousel1"G& +9 "core" ǟ& X +1889 #"nadia"-> Head.psi +2 "carousel1"G:& +8 "core" Ǻ&s +5058 #"nadia"-> Head.psi +3 "carousel1"G5& +8 "core" ǵ&|[ +901 #"nadia"-> Head.psi +3 "carousel1"Gh& +6 "core" &rW2 +718 #"nadia"-> Head.counts +2 "carousel1"GT& +7 "core" &\\ +546 "Can"mWN.zsH{ j +2406 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"GF& +8 "core" &Zz +3 "carousel1"G!& +4 "core" ǡ&\\ +3 "carousel1"Gd& +3 "core" &\\ +2 "carousel1"Ga& +5 "core" &-Z +6 "carousel1"G?& +6 "core" ǿ&[ +2 "carousel1"G & +3 "core" nj&Zp +3 "carousel1"G&& +10 "core" Ǧ&Zh +3 "carousel1"G;& +6 "core" ǻ&[ +2 "carousel1"G%& +4 "core" ǥ&[~ +2 "carousel1"G_& +4 "core" &E[ +2 "carousel1"GE& +4 "core" &vZ +2 "carousel1"GJ& +4 "core" &Y +3 "carousel1"G#& +4 "core" ǣ&[B +3 "carousel1"GM& +4 "core" &[ +2 "carousel1"G & +5 "core" Ǡ&\\Q +2 "carousel1"G& +5 "core" &A[ +2 "carousel1"GZ& +4 "core" &/\\ +2 "carousel1"G$& +4 "core" Ǥ&[ +3 "carousel1"G& +4 "core" ǜ&\\ +2 "carousel1"G1& +4 "core" DZ&]2 +4 "carousel1"G & +8 "core" Nj&\\ +2 "carousel1"G& +7 "core" Dž&[ +2 "carousel1"G& +4 "core" Ǐ&[) +2 "carousel1"G6& +4 "core" Ƕ&[ +2 "carousel1"Gx& +4 "core" &U\\/ +2 "carousel1"G<& +4 "core" Ǽ&A[1 +3 "carousel1"G0& +3 "core" ǰ&Z +3 "carousel1"G^& +4 "core" &\\ +3 "carousel1"G(& +4 "core" Ǩ&\\ +2 "carousel1"GO& +5 "core" &[ +3972 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G|& +7 "core" &<3 +3 "carousel1"G\\& +4 "core" &; +3 "carousel1"GK& +5 "core" &; +5 "carousel1"G@& +5 "core" &:M +3 "carousel1"GW& +10 "core" &= +2 "carousel1"G,& +5 "core" Ǭ&:W +3 "carousel1"G3& +3 "core" dz& d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G"& +7 "core" Ǣ&$ +3 "carousel1"GD& +4 "core" &%^ +2 "carousel1"G}& +4 "core" &% +3 "carousel1"GS& +4 "core" &&[ +2 "carousel1"G`& +5 "core" &%r +2 "carousel1"G& +5 "core" Ǒ&5& +2 "carousel1"G~& +4 "core" &$t +3 "carousel1"GP& +3 "core" && +3 "carousel1"Go& +3 "core" &$ +3 "carousel1"G& +4 "core" LJ&D% +5 "carousel1"Gr& +7 "core" &% +3 "carousel1"G/& +6 "core" ǯ&?$< +2 "carousel1"G& +4 "core" Ǖ&# +3 "carousel1"G& +4 "core" ǘ&$m +2 "carousel1"GH& +4 "core" &% +2 "carousel1"G& +4 "core" ǔ&& +2 "carousel1"G& +4 "core" ǒ&d% +3 "carousel1"Gv& +4 "core" &&# +3 "carousel1"G& +4 "core" Ǔ&%\ +2 "carousel1"G& +4 "core" ǎ&% +3 "carousel1"Gw& +4 "core" &W$ +2 "carousel1"G& +4 "core" Lj&%H +6 "carousel1"G & +5 "core" Ǎ&%| +5 "carousel1"G& +7 "core" ǐ&#o +2 "carousel1"G9& +7 "core" ǹ&%t +2 "carousel1"G\ & +4 "core" NJ&}% +3 "carousel1"G>& +3 "core" Ǿ&'( +3 "carousel1"GN& +4 "core" &$v +2 "carousel1"G8& +4 "core" Ǹ&$ +3 "carousel1"Gf& +4 "core" &Y%T +2259 "Can"kV.zsJl  +286 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G& +6 "core" Ǜ& +3 "carousel1"G& +5 "core" ǃ&2 +2 "carousel1"Gc& +5 "core" & +2 "carousel1"G& +5 "core" dž& +3 "carousel1"G*& +4 "core" Ǫ& +2 "carousel1"G& +4 "core" Ǚ&t +3 "carousel1"G& +5 "core" ǁ& +5 "carousel1"G{& +6 "core" &m +2 "carousel1"GR& +6 "core" &| +2 "carousel1"Gk& +4 "core" & +3 "carousel1"Gu& +4 "core" &h +2 "carousel1"Gz& +4 "core" &8> +3 "carousel1"G)& +4 "core" ǩ& +2 "carousel1"GI& +4 "core" & +3 "carousel1"Gb& +3 "core" & +3 "carousel1"Gy& +5 "core" &+ +2 "carousel1"Ge& +4 "core" & +2 "carousel1"GX& +4 "core" &t +3 "carousel1"G4& +4 "core" Ǵ&w +2 "carousel1"G.& +4 "core" Ǯ&rM +5 "carousel1"G& +8 "core" ǚ&n +3 "carousel1"G& +6 "core" ǟ& +2 "carousel1"G:& +4 "core" Ǻ&n +2 "carousel1"G5& +4 "core" ǵ& +2 "carousel1"Gh& +4 "core" &t +3 "carousel1"GT& +4 "core" & +3 "carousel1"GF& +8 "core" & +2 "carousel1"G!& +3 "core" ǡ&H +3 "carousel1"Gd& +4 "core" & +3 "carousel1"Ga& +5 "core" &4 +1860 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +2 "carousel1"G?& +7 "core" ǿ& +3 "carousel1"G & +4 "core" nj& +2 "carousel1"G&& +4 "core" Ǧ& +3 "carousel1"G;& +4 "core" ǻ& +3 "carousel1"G%& +5 "core" ǥ& +2 "carousel1"G_& +4 "core" & +3 "carousel1"GE& +3 "core" &9 +5 "carousel1"GJ& +8 "core" & +2 "carousel1"G#& +6 "core" ǣ& +2 "carousel1"GM& +4 "core" &o +2 "carousel1"G & +5 "core" Ǡ&} +2 "carousel1"G& +4 "core" & +3 "carousel1"GZ& +4 "core" & +2 "carousel1"G$& +5 "core" Ǥ& +2 "carousel1"G& +4 "core" ǜ& +2 "carousel1"G1& +8 "core" DZ&f +3 "carousel1"G & +3 "core" Nj& +3 "carousel1"G& +5 "core" Dž& +2 "carousel1"G& +4 "core" Ǐ&\ z +3 "carousel1"G6& +3 "core" Ƕ& +4 "carousel1"Gx& +8 "core" & +2 "carousel1"G<& +6 "core" Ǽ&H +2 "carousel1"G0& +4 "core" ǰ& +2 "carousel1"G^& +4 "core" &/d +3 "carousel1"G(& +3 "core" Ǩ& +4 "carousel1"GO& +3 "core" & +3 "carousel1"G|& +4 "core" & +2 "carousel1"G\\& +4 "core" &} +3 "carousel1"GK& +4 "core" &m +2 "carousel1"G@& +4 "core" &( +1206 #"nadia"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +767 #-> Power.sampler :ON +2 .Gate.power :sampler,:ON +2102 #-> d=0;30.times{d+=Head.counts};(d/30.0).round +5 "carousel1"GW& +4 "core" & +4 "carousel1"G,& +14 "core" Ǭ& +2 "carousel1"G3& +6 "core" dz& @ +2 "carousel1"GG& +4 "core" & ? +4 "carousel1"G[& +4 "core" &\ [ +2 "carousel1"GL& +4 "core" &] +3 "carousel1"GY& +3 "core" & +3 "carousel1"GQ& +5 "core" & +2 "carousel1"Gs& +5 "core" &\ +2 "carousel1"Gp& +3 "core" &E  +3 "carousel1"Gm& +4 "core" & x +3 "carousel1"Gg& +4 "core" &E +2 "carousel1"Gi& +4 "core" & = +3 "carousel1"G& +4 "core" DŽ&  +3 "carousel1"G7& +7 "core" Ƿ& h +2 "carousel1"GU& +6 "core" &\ +2 "carousel1"G & +4 "core" lj& a +2 "carousel1"G]& +4 "core" &a N +3 "carousel1"GC& +5 "core" & +2 "carousel1"GV& +4 "core" &  +3 "carousel1"Gq& +5 "core" & +2 "carousel1"GA& +4 "core" &t o +2 "carousel1"G2& +4 "core" Dz&e N +2 "carousel1"Gn& +4 "core" &q T +3 "carousel1"G& +5 "core" ǂ& +2 "carousel1"Gt& +5 "core" & +2 "carousel1"G"& +3 "core" Ǣ& | +4 "carousel1"GD& +8 "core" &\ +2 "carousel1"G}& +10 "core" & \\ +3 "carousel1"GS& +3 "core" & % +1228 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +2 "carousel1"G`& +8 "core" & +3 "carousel1"G& +4 "core" Ǒ& +2 "carousel1"G~& +4 "core" & +2 "carousel1"GP& +4 "core" &g +3 "carousel1"Go& +4 "core" & +3 "carousel1"G& +4 "core" LJ&g4 +2 "carousel1"Gr& +4 "core" & +3 "carousel1"G/& +3 "core" ǯ&z +3 "carousel1"G& +4 "core" Ǖ& +3 "carousel1"G& +4 "core" ǘ& +3 "carousel1"GH& +8 "core" & +2 "carousel1"G& +6 "core" ǔ&x +2 "carousel1"G& +4 "core" ǒ& +3 "carousel1"Gv& +4 "core" &] +2 "carousel1"G& +4 "core" Ǔ& +3 "carousel1"G& +3 "core" ǎ&S +3 "carousel1"Gw& +5 "core" &x +2 "carousel1"G& +4 "core" Lj& +2 "carousel1"G & +4 "core" Ǎ& +2 "carousel1"G& +4 "core" ǐ& +3 "carousel1"G9& +3 "core" ǹ&1 +3 "carousel1"G\ & +4 "core" NJ& +3 "carousel1"G>& +4 "core" Ǿ& +8 "carousel1"GN& +6 "core" &x +2 "carousel1"G8& +6 "core" Ǹ&r +2 "carousel1"Gf& +4 "core" & +3 "carousel1"G& +4 "core" Ǜ& +2 "carousel1"G& +4 "core" ǃ& +3 "carousel1"Gc& +3 "core" &q +2 "carousel1"G& +4 "core" dž&X +1132 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G*& +7 "core" Ǫ&] +3 "carousel1"G& +7 "core" Ǚ&? +3 "carousel1"G& +4 "core" ǁ&| +2 "carousel1"G{& +6 "core" &  +2 "carousel1"GR& +4 "core" & +2 "carousel1"Gk& +5 "core" &q +4 "carousel1"Gu& +7 "core" &x +2 "carousel1"Gz& +7 "core" &vA +2 "carousel1"G)& +4 "core" ǩ&r3 +63 "carousel1"GI& +3 "core" & +3 "carousel1"Gb& +8 "core" & +2 "carousel1"Gy& +16 "core" & +2 "carousel1"Ge& +4 "core" &8 +3 "carousel1"GX& +4 "core" &6 +2 "carousel1"G4& +4 "core" Ǵ& +3 "carousel1"G.& +5 "core" Ǯ&]K +2 "carousel1"G& +4 "core" ǚ& +2 "carousel1"G& +5 "core" ǟ&W +2 "carousel1"G:& +4 "core" Ǻ& +7 "carousel1"G5& +5 "core" ǵ& +2 "carousel1"Gh& +3 "core" & +3 "carousel1"GT& +4 "core" & +7 "carousel1"GF& +3 "core" & +3 "carousel1"G!& +6 "core" ǡ& +2 "carousel1"Gd& +4 "core" &a +3 "carousel1"Ga& +4 "core" &: +3 "carousel1"G?& +5 "core" ǿ&d +2 "carousel1"G & +4 "core" nj& +2 "carousel1"G&& +4 "core" Ǧ&KS +3 "carousel1"G;& +4 "core" ǻ&< +773 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G%& +8 "core" ǥ&c +2 "carousel1"G_& +4 "core" & +5 "carousel1"GE& +7 "core" & +3 "carousel1"GJ& +6 "core" & +2 "carousel1"G#& +4 "core" ǣ& +2 "carousel1"GM& +4 "core" & +2 "carousel1"G & +4 "core" Ǡ& +3 "carousel1"G& +4 "core" & +3 "carousel1"GZ& +3 "core" & +3 "carousel1"G$& +3 "core" Ǥ& +4 "carousel1"G& +3 "core" ǜ& +3 "carousel1"G1& +5 "core" DZ& +2 "carousel1"G & +4 "core" Nj& +3 "carousel1"G& +4 "core" Dž& +2 "carousel1"G& +4 "core" Ǐ&+ +3 "carousel1"G6& +8 "core" Ƕ& +2 "carousel1"Gx& +6 "core" & +2 "carousel1"G<& +4 "core" Ǽ& +3 "carousel1"G0& +3 "core" ǰ& +3 "carousel1"G^& +8 "core" & +2 "carousel1"G(& +4 "core" Ǩ& +3 "carousel1"GO& +4 "core" &t +2 "carousel1"G|& +4 "core" && +3 "carousel1"G\\& +4 "core" &p/ +2 "carousel1"GK& +4 "core" &H +2 "carousel1"G@& +4 "core" &X +3 "carousel1"GW& +5 "core" &O +2 "carousel1"G,& +4 "core" Ǭ& +3 "carousel1"G3& +9 "core" dz& +2 "carousel1"GG& +5 "core" & +371 #"PROCESSING"SPR analysis completed +576 #"SPRlogger"Ended log spr26jul3118h.spr.bz2 +3 ."PROCESSING"SPR.command "stats_off" +30 "carousel0"F[% +3 "core" %&{\ \  +7 "sampler0"HL% +6 "core" %#~o +3 #"nadia"-> Power.sampler :OFF +4 .Gate.power :sampler,:OFF +9 ."closeHandoff"Handoff.select :close +8 "valve0"D +51 ."detached from nadia#3"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +6 "valve1"EY& +6 "core" & N;Om  +4 ."detached from nadia#3"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from nadia#3"PinPort.seek :clear +3 "valve1"EQ( +81 "core" ( +2 ."detached from nadia#3"Carousel.in :default +2 "carousel0"F><` F 22 +9 Fs% +2 "core" %&N \  +4 ."detached from nadia#3"Carousel.home.seek 40 +3 "Can"jV.ztIh  +4 "carousel0"Fp'& +15 "core" ' +3 "carousel0"Fm% +4 "core" %&7 2 +2 "syringe1"Cg& +4 "core" &?t~y +3 ."detached from nadia#3"Plunge.seek :locked +4 "valve0"Di'Z +8 "core" ' +3 "syringe0"B& +4 "core" „&* zv +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +5 "syringe1"C7% +9 ."Archive"Archive.seek :clear +3 "syringe0"BU(j +7 "core" ÷%݇/;t +4 ."Air"Air.in :unlocking +3 "syringe1"C & +5 C> P]z-2 +8 "core" É&?wx +2 ."Air"Air.seek :clear +6 "syringe1"C]' +6 ."Lysis1"Lysis1.in :unlocking +11 "syringe0"BC% +3 "syringe1"C? P[(2 +74 ."Lysis1"Lysis1.seek :clear +5 "syringe1"CV( +9 "core" %L;p  +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +7 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bq'j +302 "core" ( +41 ' +42 ' +5 ( +3 "sampler0"HA% +5 "core" %# +10 ."detached from nadia#3"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +6 .Gate.power :core,:OFF +5 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785540039.75EDT @EDT1785540048.07 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +10 !o: BusOff : mesg"Icore bus was not powered while reading Intake Head Pressure's status:@timestampu: Time SʆR:@checkpoint: omitted:bt["1/home/esp/esp2/lib/i2c/sleepy.rb:500:in `<<'"1/home/esp/esp2/lib/i2c/msg.rb:779:in `write'"0/home/esp/esp2/lib/i2c/msg.rb:419:in `send'"2/home/esp/esp2/lib/i2c/servo.rb:559:in `send'"9/home/esp/esp2/lib/i2c/msg.rb:444:in `await1stReply'":/home/esp/esp2/lib/i2c/msg.rb:475:in `awaitOnlyReply'"5/home/esp/esp2/lib/i2c/servo.rb:1146:in `status'"//home/esp/esp2/lib/axis.rb:365:in `status'",/home/esp/esp2/lib/msg.rb:68:in `retry'"//home/esp/esp2/lib/axis.rb:365:in `status'"//home/esp/esp2/lib/slide.rb:59:in `status'":/home/esp/esp2/utils/cartridge/late.rb:15:in `counts'""nadia:71:in `topLevelBinding'"nadia:71:in `times'""nadia:71:in `topLevelBinding'" d=0;30.times{d+=Head.counts};(d/30.0).round +6 !o: BusOff : mesg"Icore bus was not powered while reading Intake Head Pressure's status:@timestampu: Time \\S:@checkpoint: omitted:bt["1/home/esp/esp2/lib/i2c/sleepy.rb:500:in `<<'"1/home/esp/esp2/lib/i2c/msg.rb:779:in `write'"0/home/esp/esp2/lib/i2c/msg.rb:419:in `send'"2/home/esp/esp2/lib/i2c/servo.rb:559:in `send'"9/home/esp/esp2/lib/i2c/msg.rb:444:in `await1stReply'":/home/esp/esp2/lib/i2c/msg.rb:475:in `awaitOnlyReply'"5/home/esp/esp2/lib/i2c/servo.rb:1146:in `status'"//home/esp/esp2/lib/axis.rb:365:in `status'",/home/esp/esp2/lib/msg.rb:68:in `retry'"//home/esp/esp2/lib/axis.rb:365:in `status'"//home/esp/esp2/lib/slide.rb:59:in `status'":/home/esp/esp2/utils/cartridge/late.rb:15:in `counts'""nadia:72:in `topLevelBinding'"nadia:72:in `times'""nadia:72:in `topLevelBinding'" ESP.configure +7 .Gate.power :sampler,:OFF,limit to 3500mA +4 .Gate.power :skimmer,:OFF,limit to 3500mA +5 .Gate.power :main,:ON,limit to 5000mA +6 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +235 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +32 "valve0"DP +3 D +3 "core" Ă +7 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +11 ."nadia"SamplePump.configure :default +2 "sampler0"H#^ #(22 +10 ."nadia"Toroid.configure :default + "valve0"D$ ֜2 +10 ."nadia"Carousel.configure :default +3 "carousel0"F#<` F 22 +10 ."nadia"Abs.configure :default +2 "ambient0"J#9 e  +9 ."nadia"PhaseSeparator.configure :full +4 "carousel1"G$ +9 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +8 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +7 #"nadia"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +320 #-> d=0;30.times{d+=Head.counts};(d/30.0).round +2 "carousel1"Gt& +7 "core" &O> +3 "carousel1"G"& +5 "core" Ǣ&OXp +3 "carousel1"GD& +4 "core" &O +2 "carousel1"G}& +5 "core" &OG +3 "carousel1"GS& +4 "core" &P +2 "carousel1"G`& +5 "core" &N +2 "carousel1"G& +4 "core" Ǒ&P +3 "carousel1"G~& +3 "core" &NW +3 "carousel1"GP& +5 "core" &P +2 "carousel1"Go& +4 "core" &P! +2 "carousel1"G& +4 "core" LJ&O: +3 "carousel1"Gr& +9 "core" &O] +2 "carousel1"G/& +4 "core" ǯ&NA +2 "carousel1"G& +4 "core" Ǖ&O +3 "carousel1"G& +5 "core" ǘ&O% +2 "carousel1"GH& +4 "core" &O +3 "carousel1"G& +4 "core" ǔ&P +2 "carousel1"G& +4 "core" ǒ&O +3 "carousel1"Gv& +5 "core" &O +2 "carousel1"G& +3 "core" Ǔ&O +3 "carousel1"G& +4 "core" ǎ&Owg +2 "carousel1"Gw& +5 "core" &Oi +3 "carousel1"G& +3 "core" Lj&N  +3 "carousel1"G & +4 "core" Ǎ&P{ +3 "carousel1"G& +4 "core" ǐ&P +2 "carousel1"G9& +4 "core" ǹ&OY +3 "carousel1"G\ & +4 "core" NJ&PH +2 "carousel1"G>& +4 "core" Ǿ&Nd +3 "carousel1"GN& +3 "core" &P +3 "carousel1"G8& +5 "core" Ǹ&Nw +904 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"Gf& +7 "core" &O +3 "carousel1"G& +4 "core" Ǜ&P +2 "carousel1"G& +4 "core" ǃ&O| +3 "carousel1"Gc& +4 "core" &O +2 "carousel1"G& +6 "core" dž&P +2 "carousel1"G*& +3 "core" Ǫ&Ow +3 "carousel1"G& +5 "core" Ǚ&O- +2 "carousel1"G& +3 "core" ǁ&O +2 "carousel1"G{& +4 "core" &OT{ +3 "carousel1"GR& +4 "core" &Or +2 "carousel1"Gk& +4 "core" &P, +3 "carousel1"Gu& +4 "core" &N* +2 "carousel1"Gz& +4 "core" &PA +2 "carousel1"G)& +5 "core" ǩ&O +2 "carousel1"GI& +4 "core" &Px +2 "carousel1"Gb& +4 "core" &O +3 "carousel1"Gy& +4 "core" &OF +2 "carousel1"Ge& +4 "core" &PI +3 "carousel1"GX& +3 "core" &O9K +3 "carousel1"G4& +3 "core" Ǵ&P  +3 "carousel1"G.& +4 "core" Ǯ&NN +2 "carousel1"G& +4 "core" ǚ&P +3 "carousel1"G& +4 "core" ǟ&O-o +2 "carousel1"G:& +5 "core" Ǻ&Pl +3 "carousel1"G5& +3 "core" ǵ&P) +3 "carousel1"Gh& +9 "core" &P +2 "carousel1"GT& +3 "core" &P6 +3 "carousel1"GF& +4 "core" &O-n +3 "carousel1"G!& +3 "core" ǡ&P0 +3 "carousel1"Gd& +59 "core" &P +334 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"Ga& +8 "core" &O +3 "carousel1"G?& +4 "core" ǿ&O +3 "carousel1"G & +4 "core" nj&Oq +2 "carousel1"G&& +4 "core" Ǧ&PG +3 "carousel1"G;& +4 "core" ǻ&Ov +3 "carousel1"G%& +4 "core" ǥ&O +3 "carousel1"G_& +4 "core" &P/ +2 "carousel1"GE& +4 "core" &O +3 "carousel1"GJ& +4 "core" &P +2 "carousel1"G#& +5 "core" ǣ&OYx +2 "carousel1"GM& +4 "core" &Pa +3 "carousel1"G & +4 "core" Ǡ&P +2 "carousel1"G& +4 "core" &O +3 "carousel1"GZ& +3 "core" &P| +3 "carousel1"G$& +5 "core" Ǥ&O9 +2 "carousel1"G& +3 "core" ǜ&Py{ +3 "carousel1"G1& +4 "core" DZ&Og} +2 "carousel1"G & +4 "core" Nj&Pl| +2 "carousel1"G& +4 "core" Dž&Oi +3 "carousel1"G& +4 "core" Ǐ&P? +2 "carousel1"G6& +4 "core" Ƕ&P +3 "carousel1"Gx& +4 "core" &P +2 "carousel1"G<& +4 "core" Ǽ&P +4 "carousel1"G0& +4 "core" ǰ&O +2 "carousel1"G^& +4 "core" &P6s +3 "carousel1"G(& +3 "core" Ǩ&OW +3 "carousel1"GO& +7 "core" &OA +3 "carousel1"G|& +4 "core" &O +2 "carousel1"G\\& +4 "core" &O +3 "carousel1"GK& +5 "core" &P +671 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +4 "carousel1"G@& +6 "core" &O +3 "carousel1"GW& +4 "core" &P +2 "carousel1"G,& +4 "core" Ǭ&O +3 "carousel1"G3& +5 "core" dz&P{ +2 "carousel1"GG& +3 "core" &P] +4 "carousel1"G[& +4 "core" &O +2 "carousel1"GL& +4 "core" &Pk! +3 "carousel1"GY& +4 "core" &O +3 "carousel1"Gs& +4 "core" &P6 +2 "carousel1"Gm& +5 "core" &P +2 "carousel1"Gg& +4 "core" &Oa +2 "carousel1"G& +4 "core" DŽ&P/& +2 "carousel1"G7& +4 "core" Ƿ&P +3 "carousel1"G & +4 "core" lj&Q +3 "carousel1"GC& +6 "core" &Ol +3 "carousel1"G& +4 "core" Ǟ&P% +3 "carousel1"G=& +4 "core" ǽ&Pdx +2 "carousel1"Gj& +4 "core" &P: +2 "carousel1"G-& +4 "core" ǭ&O +2 "carousel1"G& +4 "core" ǝ&O} +2 "carousel1"G+& +4 "core" ǫ&P +2 "carousel1"G& +4 "core" Ǘ&OS +3 "carousel1"GA& +4 "core" &O +3 "carousel1"G& +3 "core" ǂ&P +3 "carousel1"Gt& +4 "core" &OV +2 "carousel1"G"& +4 "core" Ǣ&P +3 "carousel1"GD& +4 "core" &OI +2 "carousel1"G}& +4 "core" &P%$ +3 "carousel1"GS& +4 "core" &O +2 "carousel1"G`& +5 "core" &O +4933 "Can"lV.z#t<h  +7797 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G& +6 "core" Ǒ&OT +3 "carousel1"G~& +4 "core" &Pla +2 "carousel1"GP& +4 "core" &O8 +3 "carousel1"Go& +4 "core" &O +2 "carousel1"G& +4 "core" LJ&Qx +2 "carousel1"Gr& +5 "core" &O +2 "carousel1"G/& +4 "core" ǯ&P +3 "carousel1"G& +4 "core" Ǖ&O8 +2 "carousel1"G& +4 "core" ǘ&PR +3 "carousel1"GH& +3 "core" &Od' +3 "carousel1"G& +4 "core" ǔ&Q +2 "carousel1"G& +4 "core" ǒ&Q +3 "carousel1"Gv& +4 "core" &P: +2 "carousel1"G& +4 "core" Ǔ&Q +3 "carousel1"G& +4 "core" ǎ&O +2 "carousel1"Gw& +4 "core" &P +3 "carousel1"G& +4 "core" Lj&P +2 "carousel1"G & +4 "core" Ǎ&PK +2 "carousel1"G& +4 "core" ǐ&P} +3 "carousel1"G9& +3 "core" ǹ&P +3 "carousel1"G\ & +4 "core" NJ&PQ +2 "carousel1"G>& +4 "core" Ǿ&P| +3 "carousel1"GN& +3 "core" &P. +3 "carousel1"G8& +5 "core" Ǹ&P9n +2 "carousel1"Gf& +4 "core" &PT +3 "carousel1"G& +5 "core" Ǜ&O +5 "carousel1"G& +4 "core" ǃ&Qj +3 "carousel1"Gc& +4 "core" &P- +2 "carousel1"G& +4 "core" dž&PB +3 "carousel1"G*& +4 "core" Ǫ&P` +679 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +2 "carousel1"G& +7 "core" Ǚ&Qh +3 "carousel1"G& +3 "core" ǁ&O +3 "carousel1"G{& +4 "core" &O +2 "carousel1"GR& +4 "core" &O +3 "carousel1"Gk& +4 "core" &P] +3 "carousel1"Gu& +3 "core" &Px +3 "carousel1"Gz& +4 "core" &P +3 "carousel1"G)& +5 "core" ǩ&P +2 "carousel1"GI& +70 "core" &PF +3 "carousel1"Gb& +4 "core" &Q +3 "carousel1"Gy& +3 "core" &P +3 "carousel1"Ge& +5 "core" &P +2 "carousel1"GX& +4 "core" &P +3 "carousel1"G4& +5 "core" Ǵ&O +2 "carousel1"G.& +4 "core" Ǯ&P: +3 "carousel1"G& +3 "core" ǚ&OF] +3 "carousel1"G& +4 "core" ǟ&ON +3 "carousel1"G:& +4 "core" Ǻ&Q +3 "carousel1"G5& +3 "core" ǵ&O +3 "carousel1"Gh& +4 "core" &QQ +3 "carousel1"GT& +4 "core" &Q +3 "carousel1"GF& +5 "core" &P +2 "carousel1"G!& +4 "core" ǡ&P +2 "carousel1"Gd& +4 "core" &P9 +3 "carousel1"Ga& +4 "core" &PG +2 "carousel1"G?& +4 "core" ǿ&PM +3 "carousel1"G & +4 "core" nj&P( +3 "carousel1"G&& +4 "core" Ǧ&P +2 "carousel1"G;& +4 "core" ǻ&O8 +2 "carousel1"G%& +4 "core" ǥ&P, +292 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +2 "carousel1"G_& +8 "core" &Pk +3 "carousel1"GE& +4 "core" &Ou +2 "carousel1"GJ& +4 "core" &P +3 "carousel1"G#& +4 "core" ǣ&O' +2 "carousel1"GM& +4 "core" &Q +3 "carousel1"G & +4 "core" Ǡ&P}} +3 "carousel1"G& +3 "core" &Pf +2 "carousel1"GZ& +4 "core" &P +2 "carousel1"G$& +5 "core" Ǥ&P} +2 "carousel1"G& +5 "core" ǜ&P! +2 "carousel1"G1& +4 "core" DZ&P +2 "carousel1"G & +5 "core" Nj&Pt +2 "carousel1"G& +4 "core" Dž&P +2 "carousel1"G& +4 "core" Ǐ&P +3 "carousel1"G6& +4 "core" Ƕ&Ohi +2 "carousel1"Gx& +3 "core" &P +3 "carousel1"G<& +4 "core" Ǽ&OB +3 "carousel1"G0& +5 "core" ǰ&Og +3 "carousel1"G^& +3 "core" &Q +6 "carousel1"G(& +5 "core" Ǩ&OL +3 "carousel1"GO& +5 "core" &P3 +5 "carousel1"G|& +10 "core" &P +4 "carousel1"G\\& +7 "core" &O +5 "carousel1"GK& +7 "core" &P +6 "carousel1"G@& +8 "core" &PW +4 "carousel1"GW& +8 "core" &Qr +5 "carousel1"G,& +7 "core" Ǭ&O +6 "carousel1"G3& +6 "core" dz&O +6 "carousel1"GG& +5 "core" &P +2 "carousel1"G[& +3 "core" &O +645 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"GL& +7 "core" &O +3 "carousel1"GY& +4 "core" &PI[ +2 "carousel1"Gs& +4 "core" &O +3 "carousel1"Gm& +4 "core" &Oy +3 "carousel1"Gg& +8 "core" &Po +2 "carousel1"G& +4 "core" DŽ&O~t +3 "carousel1"G7& +4 "core" Ƿ&Pf +2 "carousel1"G & +4 "core" lj&O +3 "carousel1"GC& +4 "core" &O+ +3 "carousel1"G& +4 "core" Ǟ&P~; +2 "carousel1"G=& +4 "core" ǽ&O +3 "carousel1"Gj& +4 "core" &P +2 "carousel1"G-& +4 "core" ǭ&O +3 "carousel1"G& +4 "core" ǝ&Pt +2 "carousel1"G+& +4 "core" ǫ&Po +3 "carousel1"G& +4 "core" Ǘ&P~L +2 "carousel1"GA& +4 "core" &PH +2 "carousel1"G& +5 "core" ǂ&PV +2 "carousel1"Gt& +4 "core" &Ple +2 "carousel1"G"& +4 "core" Ǣ&P +2 "carousel1"GD& +4 "core" &Pt+ +3 "carousel1"G}& +3 "core" &Pj +3 "carousel1"GS& +5 "core" &P5 +3 "carousel1"G`& +3 "core" &Px +3 "carousel1"G& +4 "core" Ǒ&PJ +2 "carousel1"G~& +4 "core" &P$ +3 "carousel1"GP& +4 "core" &PP +2 "carousel1"Go& +4 "core" &Qg/ +2 "carousel1"G& +4 "core" LJ&Ol_ +3 "carousel1"Gr& +4 "core" &Qx$ +1625 "Can"kW.zt@  +1300 #"nadia"-> d=0;30.times{d+=Head.counts};(d/30.0).round +3 "carousel1"G/& +7 "core" ǯ&P; +2 "carousel1"G& +4 "core" Ǖ&OI{ +3 "carousel1"G& +3 "core" ǘ&PH +3 "carousel1"GH& +4 "core" &P[ +3 "carousel1"G& +4 "core" ǔ&P1 +2 "carousel1"G& +5 "core" ǒ&P6 +2 "carousel1"Gv& +4 "core" &PX +2 "carousel1"G& +4 "core" Ǔ&P +2 "carousel1"G& +4 "core" ǎ&PQ +3 "carousel1"Gw& +4 "core" &PJi +2 "carousel1"G& +4 "core" Lj&OwI +3 "carousel1"G & +4 "core" Ǎ&Ppc +2 "carousel1"G& +4 "core" ǐ&O +2 "carousel1"G9& +4 "core" ǹ&P( +3 "carousel1"G\ & +3 "core" NJ&O*N +3 "carousel1"G>& +7 "core" Ǿ&Prx +3 "carousel1"GN& +4 "core" &Pl +3 "carousel1"G8& +4 "core" Ǹ&Pr +2 "carousel1"Gf& +63 "core" &Qg. +2 "carousel1"G& +4 "core" Ǜ&O  +3 "carousel1"G& +4 "core" ǃ&Qn9 +3 "carousel1"Gc& +4 "core" &OH +2 "carousel1"G& +4 "core" dž&Q`g +3 "carousel1"G*& +4 "core" Ǫ&Po, +3 "carousel1"G& +4 "core" Ǚ&P5 +3 "carousel1"G& +4 "core" ǁ&P; +2 "carousel1"G{& +4 "core" &P0 +3 "carousel1"GR& +3 "core" &P +3 "carousel1"Gk& +4 "core" &P= +2 "carousel1"Gu& +4 "core" &P]A +10401 "Can"jW.z#t@  +11960 lV.zt?  +8755 #"nadia"-> MainThread.exit +5 "sampler0"Hz% +3 "core" %\\( +7 #mEXIT +8 !"nadia"o:ThreadError: mesg"killed thread:@timestampu: Time w:bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +9 #"na"Session Ended +5 #"nadia"Session Ended @1785540599.39EDT @1785540609.69EDT = =mMAIN_scheduler =tMAIN @EDT1785540610.96 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +211 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +28 "Can"nV.zt?^ 7 +333 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +68 #Sea pressure (Abs) tare is 6% of full scale +376 "Can"nV.zt([ 9 + nV.zt([ 9 + #tPolling Can every 2:00 +4 #Waiting for espclients... +48 "Can"jV.zt;5 9 +67 #"nadia"Session Begun +1010 #-> ESP.ready! +18 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +58 "valve0"DP +4 D +3 "core" ā +7 "valve0"D% +3 "core" Ă%G)OU { +3 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 "sampler0"H% +3 "core" ȃ%.; +3 ."nadia"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" Ą&:OQ b +2 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +13 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +5 "carousel0"F% +7 "core" ƅ%PU X +3 ."nadia"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 "ambient0"J% +3 "core" ʆ% +3 ."nadia"Abs.configure :default +2 "ambient0"J#  +8 "carousel1"G& +3 "core" LJ&P +3 ."nadia"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B& +4 "core" ˆ&Aju  +2 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 "syringe1"C % +3 "core" É%ݞHt +3 ."nadia"Air.configure :default + "syringe1"C#d P]z<2 +7 C\ & +3 "core" Ê&z0h : +3 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B % +3 "core" ‹%٧,  +2 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 "valve1"E & +3 "core" Ō&On , +2 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +7 #"nadia"All elf controllers running firmware version 4.94 +5 "valve0"D % +5 "core" č%G$Os  +3 "valve0"D& +7 "carousel0"F% +5 ."Plunge_home"Plunge.in :default +11 "syringe0"B& +7 "syringe1"C% +8 C& +2 "valve0"D> P ޤ-2 +8 "syringe0"B% +4 "valve1"E& +16 ."Plunge_home"Plunge.home.jog 0 +2 "valve0"D) +5 "core" Ď&BOu Z + Ə%Qi X + &Ek  + Ñ%ݠg +2 Ò&p/t D “%٣dz  + Ŕ&Oq , + ĕ) +9 ."PinPort_home"PinPort.in :default +8 ."Lysis1_home"Lysis1.in :default +4 ."Air_home"Air.in :default +4 ."Carousel_home"Carousel.in :default +6 ."Toroid_home"Toroid.in :default +3 "valve0"D% +3 ."Lysis2_home"Lysis2.in :default +6 "valve1"E? B22 +4 ."Archive_home"Archive.in :default +6 "syringe1"C?d P[<2 +6 C>d P]z<2 + "carousel0"F><` F 22 +2 "valve0"D? ֜2 +4 "syringe0"B>d P\\k<2 + B?d PZ<2 +5 ."PinPort_home"PinPort.home.jog 0 +12 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Air_home"Air.home.jog 0 +3 "valve1"E* +6 ."Lysis2_home"Lysis2.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +5 "syringe1"C* +2 C) +3 "syringe0"B) +8 B* +3 "carousel0"F) + "valve0"D* +20 "core" Ė%PGOw  + ŗ* Ø* + Ù) + š) + ›* ĝ* + Ɯ) +4 "valve1"E& +6 "syringe0"B% +4 "syringe1"C!% +11 C & +2 "valve0"D"& +2 "syringe0"B#& +4 ."Plunge_home"Plunge.in :homing +19 "valve0"D> (2 +7 "core" Ş&Oi + Ÿ%t٫jc + á%ݠO + à&u.Y + Ģ&?Oh + £&z79O  +5 ."Air_home"Air.in :homing +11 ."Plunge_home"Plunge.jog 1483 +4 ."Lysis2_home"Lysis2.in :homing +5 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +4 ."PinPort_home"PinPort.in :homing +4 "syringe1"C> -2 +3 "valve0"D$) +2 "syringe0"B> (2 +3 "valve0"D?2 +7 "syringe1"C? (2 + "syringe0"B? -2 +12 "valve1"E?2 +52 ."Air_home"Air.jog 1427 +7 ."Lysis1_home"Lysis1.jog 1596 +2 ."Toroid_home"Toroid.jog 1352 +5 ."Lysis2_home"Lysis2.jog 1617 +5 ."Archive_home"Archive.jog 1614 +4 ."PinPort_home"PinPort.jog 1232 +5 "syringe1"C%) +3 C&*< +6 "valve0"D'*H +7 "syringe0"B()Q +2 B)*N +2 "valve1"E** +21 "core" Ĥ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"D+' +25 "core" ī' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +34 "core" å) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +6 ."Air_home"Air.seek :home +3 "syringe1"C,' +6 "core" æ* +5 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +6 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C-( +5 "core" ì' + ¨) + ©* +7 ."Air_home"Air.in :default +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe1"C>d P]z<2 +3 "syringe0"B72 +4 B82 +6 ."Lysis2_home"Lysis2.seek :home +4 ."Archive_home"Archive.seek :home +5 "syringe0"B.' +5 B/( +13 "core" í( + Ū* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +3 ."Lysis1_home"Lysis1.in :default +4 "valve1"E8 N +3 "syringe1"C?d P[<2 +9 ."PinPort_home"PinPort.seek :home +4 "valve1"E0( +8 "core" ®' ¯( +3 ."Archive_home"Archive.in :default +4 ."Lysis2_home"Lysis2.in :default +4 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +203 "core" ħ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D1( +7 "core" ı( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +186 "core" Ű( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +4 "valve1"E2& +4 "valve0"D3( +5 "core" Ų&©Oa W +6 ."nadia"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."nadia"PinPort.seek :clear +3 "valve1"E4( +42 "core" Ŵ( +290 ij( +2 ."nadia"Air.seek :clear +3 "syringe1"C5' +7 "core" õ' +3 ."nadia"Lysis1.seek :clear +2 "syringe1"C6( +69 "core" ö( +2 ."nadia"Lysis2.seek :clear +3 "syringe0"B7'j +76 "core" ·' +3 ."nadia"Archive.seek :clear +3 "syringe0"B8(j +75 "core" ¸( +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F9% +3 "core" ƹ%&XZ  +4 ."nadia"Carousel.home.seek 40 +4 "carousel0"F:'& +16 "core" ƺ' +2 "valve0"D;% +4 "core" Ļ%^ Oz !{ +3 "valve0"D<% +4 "core" ļ%^Oy !c +4 "sampler0"H=% +4 "core" Ƚ%8Q +4 "valve0"D>& +4 "core" ľ&' Ow +3 "carousel0"F?% +4 "core" ƿ%&Q  +3 "ambient0"J@% +7 "core" % +4 "carousel1"GA& +5 "core" &Pl +2 "syringe0"BB& +4 "core" &j2Qc +3 "syringe1"CC% +4 "core" %ݪM +3 "syringe1"CD& +4 "core" &ّPl +3 "syringe0"BE% +4 "core" %jL +3 "valve1"EF& +4 "core" &COr  +137 #"nadia"-> Head.psi +2 "carousel1"GG& +5 "core" &P +729 #"nadia"-> Head.psi +4 "carousel1"GH& +3 "core" &Qe +268 #"nadia"-> Head.psi +3 "carousel1"GI& +9 "core" &Pi +449 #"nadia"-> Head.counts +2 "carousel1"GJ& +6 "core" &Qt. +2400 #"nadia"-> Head.counts +2 "carousel1"GK& +5 "core" &Q +3274 #"nadia"-> MainThread.exit +4 "sampler0"HL% +3 "core" %9 +4 ."closeIntake"Intake.close +10 ."closeExhaust"Exhaust.close +4 ."closeBypass"Bypass.close +5 "sampler0"HM/ +3 HN0 +2 "sampler1"IO/ +16 "core" / 0 + / +11 #mEXIT +2 #"nadia"Session Ended @1785540728.39EDT @1785540737.85EDT = =tMAIN =mMAIN_scheduler @EDT1785540739.05 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +155 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +26 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +32 "Can"mV.zt=Ub W +331 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +67 #Sea pressure (Abs) tare is 6% of full scale +368 "Can"mV.zt,K Y + mV.zt,K Y + #tPolling Can every 2:00 +5 #Waiting for espclients... +47 "Can"kV.zt@v Y +69 #"nadia"Session Begun +158 #-> ESP.ready! +18 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +56 "valve0"DP +5 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +4 "valve0"D +4 "core" ā +6 "valve0"D% +4 "core" Ă%^OP !i +3 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +8 "sampler0"H% +3 "core" ȃ%D +3 ."nadia"SamplePump.configure :default +2 "sampler0"H#^ #(22 +10 "valve0"D& +3 "core" Ą&,OR +3 ."nadia"Toroid.configure :default + "valve0"D$ ֜2 +7 ."nadia"Toroid.hold +2 "valve0"D, +5 "core" ą, +3 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +9 "carousel0"F% +3 "core" Ɔ%&O-  +2 ."nadia"Carousel.configure :default +2 "carousel0"F#<` F 22 +7 ."nadia"Carousel.hold +2 "carousel0"F+ +5 "core" Ƈ+ +2 ."nadia"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 "ambient0"J% +3 "core" ʈ% +3 ."nadia"Abs.configure :default +2 "ambient0"J#  +9 "carousel1"G & +3 "core" lj&Ou +2 ."nadia"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B\ & +3 "core" Š&j*}p +3 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."nadia"Archive.hold +2 "syringe0"B , +8 "core" ‹, +4 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 "syringe1"C % +3 "core" Ì%ݵ3] +4 ."nadia"Air.configure :default + "syringe1"C#d P]z<2 +8 ."nadia"Air.hold +2 "syringe1"C + +5 "core" Í+ +3 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 C& +4 "core" Î&ٍ*e +3 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."nadia"Lysis1.hold +2 "syringe1"C, +6 "core" Ï, +3 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 "syringe0"B% +4 "core" %jپ/S +3 ."nadia"Lysis2.configure :default + "syringe0"B#d P\\k<2 +8 ."nadia"Lysis2.hold +2 "syringe0"B+ +5 "core" ‘+ +3 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 "valve1"E& +4 "core" Œ&>Oi  +2 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +7 ."nadia"PinPort.hold +2 "valve1"E, +5 "core" œ, +3 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +6 #"nadia"All elf controllers running firmware version 4.94 +6 "valve0"D% +4 D& +5 "carousel0"F% +9 "core" Ĕ%^\ Oj  + "syringe0"B& +7 "core" ĕ&#O` +2 "syringe1"C% +2 "core" Ɩ%&P+  +4 "syringe1"C& +9 "syringe0"B% +7 "valve1"E& +16 "core" —&j5cc + Ø%ݷek + Ù&ٖ/R + š%j,X + ś&; Og  +8 ."clearToroid"Toroid.seek :clear +3 "valve1"E& +3 "valve0"D( +10 "core" Ŝ&DOf  + ĝ( +4 ."nadia"Air.seek :clear +3 "syringe1"C' +6 "core" Þ' +3 ."nadia"Lysis1.seek :clear +3 "syringe1"C( +5 "core" ß( +3 ."nadia"Lysis2.seek :clear +4 "syringe0"B 'j +5 "core"  ' +4 ."nadia"Archive.seek :clear +3 "syringe0"B!(j +6 "core" ¡( +2 ."nadia"Carousel.in :default +4 "carousel0"F><` F 22 +6 F"% +3 "core" Ƣ%&P  +44 ."nadia"Carousel.home.seek 40 +4 "carousel0"F#'& +26 "core" ƣ' +3 "valve0"D$% +8 "core" Ĥ%^Oc  +3 "valve0"D%% +4 "core" ĥ%^Od +4 "sampler0"H&% +4 "core" Ȧ%NL +4 "valve0"D'& +5 "core" ħ&.Oe +3 "carousel0"F(% +4 "core" ƨ%&Q  +3 "ambient0"J)% +5 "core" ʩ% +2 "carousel1"G*& +4 "core" Ǫ&QJ +3 "syringe0"B+& +5 "core" «&j1cC +2 "syringe1"C,% +4 "core" ì%ݧˁ6 +4 "syringe1"C-& +4 "core" í&ّaX +3 "syringe0"B.% +4 "core" ®%jn- +3 "valve1"E/& +4 "core" ů&AOd  +223 #"nadia"-> Head.psi +3 "carousel1"G0& +5 "core" ǰ&P +816 #"nadia"-> Power.sampler :ON +4 .Gate.power :sampler,:ON +1460 #-> Head.psi +8 "carousel1"G1& +9 "core" DZ&Qq- +1135 #"nadia"-> Head.psi +3 "carousel1"G2& +5 "core" Dz&P\  +944 #"nadia"-> Head.psi +4 "carousel1"G3& +6 "core" dz&Q( +798 #"nadia"-> Head.psi +3 "carousel1"G4& +5 "core" Ǵ&Q\\ +810 #"nadia"-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +4744 "Can"jWh.zt># w +11962 mW`.z(t? +116 #"nadia"-> slot +11844 "Can"lW.zt>E +8268 #"nadia"-> #slot 40 (L27) & slot 50/51 (A53) cool L.Huron water +di 50/50 +3692 "Can"iW2.z&t@o Ѽ +1003 #"nadia"-> slot 30=>:lyseHold_phyto +3304 #-> #L. Huron microsystin water now being used +1870 #-> #SC 30 has L72 +529 #-> slot +5252 "Can"lW.zt= +5110 #"nadia"-> # SC45 A33 +5997 #-> Cart.unlock +5 "valve1"E5& +3 "core" ŵ&>Od  +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F6% +3 "core" ƶ%&P?F  +4 ."nadia"Carousel.home.seek 40 +4 "carousel0"F7'& +14 "core" Ʒ' +3 "carousel0"F8% +3 "core" Ƹ%&P\\?  +3 "syringe0"B9& +4 "core" ¹&j,) +3 "syringe1"C:% +5 "core" ú%ݧ~" +2 "syringe0"B;% +5 "core" »%jٲB +2 "syringe1"C<& +5 "core" ü&ي, +2 "valve0"D=% +5 "core" Ľ%^O^ +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +2 "valve0"D>'8 +45 "core" ľ' +291 #"nadia"-> SC.to 20 +3 .Carousel.seek 20 +6 "carousel0"F?' +406 "Can"mW.zt' +23 "core" ƿ' +2 "carousel0"F@% +5 "core" %;Sg 2 +2153 #"nadia"-> #SC20 L79 +815 #-> Cart.dry +2673 #-> MainThread.exit +4 "sampler0"HA% +4 "core" %V +3 ."closeIntake"Intake.close +8 ."closeExhaust"Exhaust.close +5 ."closeBypass"Bypass.close +5 "sampler0"HB/ +3 HC0 +4 "sampler1"ID/ +15 "core" / + 0 + / +10 #mEXIT +2 #"nadia"Session Ended @1785541524.38EDT @1785541535.49EDT = =tMAIN =mMAIN_scheduler @EDT1785541536.74 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +140 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +30 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +28 "Can"nW8.z(t7  +326 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +365 "Can"nW8.z(t$ ! +2 nW8.z(t$ ! + #tPolling Can every 2:00 +4 #Waiting for espclients... +47 "Can"lW3.z't=K ! +93 #"preston"Session Begun +11865 "Can"lV.zt;b+ ? +5031 #"preston"-> start {fire lysis, archive, hold} +36 ."detached from preston"Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +54 "valve0"DP +4 D +2 "core" ā +7 "valve0"D% +3 "core" Ă%8ZOU +3 ."detached from preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +7 ."detached from preston"Plunge.hold +4 "valve0"D+ +6 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +4 "core" ă+ +3 ."detached from preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +12 "sampler0"H% +3 "core" Ȅ%tH +2 ."detached from preston"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 "valve0"D& +3 "core" ą&/OP +3 ."detached from preston"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."detached from preston"Toroid.hold +2 "valve0"D, +5 "core" Ć, +3 ."detached from preston"Toroid.configure :default +2 "valve0"D$ ֜2 +8 "carousel0"F% +3 "core" Ƈ%ZSgJ  +3 ."detached from preston"Carousel.configure :default +3 "carousel0"F#<` F 22 +6 ."detached from preston"Carousel.hold +2 "carousel0"F+ +5 "core" ƈ+ +3 ."detached from preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 "ambient0"J % +3 "core" ʉ% +3 ."detached from preston"Abs.configure :default +2 "ambient0"J#  +9 "carousel1"G\ & +3 "core" NJ&Z +3 ."detached from preston"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B & +3 "core" ‹&j5C +3 ."detached from preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."detached from preston"Archive.hold +2 "syringe0"B , +5 "core" Œ, +3 ."detached from preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 "syringe1"C % +3 "core" Í%ݼ. +3 ."detached from preston"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from preston"Air.hold +2 "syringe1"C+ +5 "core" Î+ +4 ."detached from preston"Air.configure :default +2 "syringe1"C#d P]z<2 +8 C& +5 "core" Ï&ٗ8) +3 ."detached from preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from preston"Lysis1.hold +2 "syringe1"C, +5 "core" Ð, +4 ."detached from preston"Lysis1.configure :default + "syringe1"C$d P[<2 +6 "syringe0"B% +4 "core" ‘%jٹ3 +2 ."detached from preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +7 ."detached from preston"Lysis2.hold +2 "syringe0"B+ +5 "core" ’+ +3 ."detached from preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 "valve1"E& +4 "core" œ&EOP  +2 ."detached from preston"PinPort.configure :default +2 "valve1"E$ B22 +7 ."detached from preston"PinPort.hold +2 "valve1"E, +7 "core" Ŕ, +2 ."detached from preston"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from preston"All elf controllers running firmware version 4.94 +7 "valve0"D% +5 D& +3 "carousel0"F% +6 "syringe0"B& +5 "syringe1"C% +4 C& +7 "syringe0"B% +5 "valve1"E& +3 "core" ĕ%8ZOS +27 Ė&$OU +2 Ɨ%ZSf&7  + ˜&j00 + Ù%ݵi* + Ú&ّ5 Ŝ&AON  + ›%j4& +11 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +6 "valve0"D( +9 "core" ŝ&CjOO  + Ğ( +5 ."detached from preston"Air.seek :clear +3 "syringe1"C' +43 "core" ß' +4 ."detached from preston"Lysis1.seek :clear +2 "syringe1"C ( +7 "core" à( +3 ."detached from preston"Lysis2.seek :clear +3 "syringe0"B!'j +7 "core" ¡' +2 ."detached from preston"Archive.seek :clear +3 "syringe0"B"(j +7 "core" ¢( +2 ."detached from preston"Carousel.in :default +3 "carousel0"F><` F 22 +6 F#% +3 "core" ƣ%YSf9  +4 ."detached from preston"Carousel.home.seek 20 +3 "carousel0"F$' +16 "core" Ƥ' +2 "valve0"D%% +5 "core" ĥ%8ZOy +10 #"detached from preston"Selecting Cartridge 20 +2 "valve0"D&% +8 "core" Ħ%8ZOv +2 "syringe1"C'& +5 "core" ç&ُhQ +3 "syringe1"C(% +4 "core" è%ݸgZ +3 "valve1"E)& +5 "core" ũ&9Ot  +2 ."detached from preston"Carousel.seek 20 +3 "carousel0"F*' +17 "core" ƪ' +4 "carousel0"F+% +4 "core" ƫ%PSc  +8 ."detached from preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C,& +4 "core" ì&هfG +4 ."detached from preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from preston"Lysis1.seek :locked +3 "syringe1"C-( +138 "core" ƪ' TSN!%  +3 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 20 - 5 counts (sensed 20 - 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi:@pastThresholdF: @voltagef14.70990083456063: @currentiY: @type2F:@outPressi%!:\ @lostF: @inPressiNS:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @tagi/: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ƪ' +107 í( +4 "valve0"D.% +8 "core" Į%8ZO{ +2 ."Plunge"Plunge.seek :locked +3 "valve0"D/'Z +8 "syringe1"C0% +7 "core" ð%ݵd +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +2 "syringe1"C1'6 +70 "core" į' +34 ñ' +2 ."detached from preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F2% +3 "core" Ʋ%]S_[  +6 #"detached from preston"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from preston"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from preston"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from preston"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" 3t +5 "cartridge" t +3 "Cartridge" 4y +202 "cartridge" yՎՎ +3 "Cartridge" 5y  +4 "cartridge" y /J59971 Z/MC% +4 #"detached from preston"Mounted lysis Cartridge board#J59971 +10 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"D6% +5 "core" Ķ%ZG)O_ 3 +3 "syringe1"C7& +5 "core" ÷&\\- o +2 "syringe1"C8% +4 "core" ø%6, & +2 "carousel0"F9% +5 "core" ƹ%S^v \  +3 #"FILTERING"Preparing to filter microSPR_phyto cartridge in slot 20 +5 "valve0"D:% +6 "core" ĺ%ZG)O] 3 +3 "syringe1"C;& +4 "core" û&zY) z +3 "syringe1"C<% +4 "core" ü%6/Z: # +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G=& +6 "core" ǽ& +45 "carousel1"G>& +3 "core" Ǿ& +47 "carousel1"G?& +3 "core" ǿ&` +47 "carousel1"G@& +4 "core" & +46 "carousel1"GA& +3 "core" & +47 "carousel1"GB& +3 "core" &d +47 "carousel1"GC& +3 "core" &qP +47 "carousel1"GD& +3 "core" &yH +48 "carousel1"GE& +3 "core" &C +47 "carousel1"GF& +4 "core" & +46 "carousel1"GG& +3 "core" &| +47 "carousel1"GH& +3 "core" & +47 "carousel1"GI& +4 "core" & +46 "carousel1"GJ& +3 "core" & +5 "Can"lV.zt2M R + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 29C (drifted 6%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +6 .PhaseSeparator.in :filling +2 "carousel1"G?"d"d +6 ."FILTERING"PhaseSeparator.hold + "carousel1"GK, +6 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +666 "core" ,e4 +7 #"FILTERING"Head pressure reached 3.95psi in 7 seconds +5 ."sealing"Air.in :default +3 "syringe1"C>d P]z<2 +5 "carousel0"FL% +6 "core" %S]d* \  +2 ."sealing"Air.seek :sealed +5 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +4 "syringe1"CM' +5 ."FILTERING"SamplePump.setPosition! 0ml +4 "sampler0"H7 +7 ."FILTERING"Exhaust.close +2 "sampler0"HN0 +6 "core" 0 +3 ."FILTERING"Bypass.open +3 "sampler1"IO1 +154 "core" 1 +73 "ambient0"JP% +4 "core" % x +45 ."FILTERING"Intake.open +2 "sampler0"HQ1 +7 "core" ' +139 1 +3 "ambient0"JR% +5 "core" % +14 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +4 "sampler0"HS% +6 "core" %GZ +43 "sampler0"HT% +4 "core" %BO +46 "sampler0"HU% +3 "core" %=] +47 "sampler0"HV% +4 "core" %5` +46 "sampler0"HW% +3 "core" %9P +47 "sampler0"HX% +4 "core" %9N +8 "Can"lV.zs<N VP +2 #"FILTERING"Vacuum tare is 54% of full scale at 29C (drifted 2%) +2 "ambient0"JY% +9 "core" % +2 "ambient0"JZ% +5 "core" % +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J[+ +6 "core" + +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G\\, +7 "core" , +4 "sampler0"H]% +4 "core" %d +3 "sampler0"H^% +5 "core" %o +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "19:50:01.91" +2898 "Can"nV.zs< ] +1585 nV.zs< a +3 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n#(}@$3 22Q +8 ."FILTERING"SamplePump.seek 10ml,1:10 +2 "sampler0"H_'6 +58 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(}@$3 22Q +1283 "core" ' +2 "sampler0"H`% +5 "core" %6k 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 ."FILTERING"Exhaust.open +2 "sampler0"Ha2 +152 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"Ib/ +153 "core" / +74 "sampler0"Hc% +3 "core" %l +2 "ambient0"Jd% +5 "core" % +4 ."FILTERING"Abs.in :default + "ambient0"J>9 e  +7 ."FILTERING"Abs.hold +2 "ambient0"Je+ +5 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gf, +7 "core" , +2 "ambient0"Jg% +4 "core" % +3 "sampler0"Hh% +4 "core" %d +3 "sampler0"Hi% +5 "core" %` +4 ."FILTERING"SamplePump.in :start +4 "sampler0"H>^ }n#(}@?$3 22Q +6 ."FILTERING"SamplePump.seek 200ml,13:50 +2 "sampler0"Hj'G +58 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(}@?$3 22Q +8385 "Can"kV&.zsUUȋ { +11959 iU.zsRUҏ +5860 "core" ' +2 "sampler0"Hk% +4 "core" %G 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Bypass.open +2 "sampler1"Il1 +150 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"Hm/ +146 "core" / +2 "carousel0"Fn% +4 "core" %S]'c \  +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :crack +3 "valve0"Do( +170 "core" ( +78 ."FILTERING"Toroid.seek :cartridge +2 "valve0"Dp(m +208 "core" ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +2 "valve0"Dq( +373 "core" ( +2 "ambient0"Jr% +4 "core" % +5 "Can"iU.zsvH H +2 "ambient0"Js% +3 "core" %1 +2 "ambient0"Jt% +4 "core" %0 +3 #"FILTERING"Pressurizing cartridge to 14psi above 15.4psia +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +6 ."FILTERING"Intake.open + "sampler0"Hu1 +147 "core" 1 +2 ."FILTERING"Bypass.close +2 "sampler1"Iv/ +151 "core" / +2 ."FILTERING"Exhaust.close +3 "sampler0"Hw0 +151 "core" 0 +3 "sampler0"Hx% +4 "core" %̈ +3 "ambient0"Jy% +4 "core" % +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"Jz+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G{, +7 "core" , +3 "sampler0"H|% +5 "core" %Lj +2 "sampler0"H}% +46 "core" %ˆ +4 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>nu0L3 22Q +16 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +3 "sampler0"H~' +1117 "core" 'W 2 +5 "sampler0"H% +3 "core" % 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Intake.close +2 "sampler0"H/ +148 "core" ȁ/ +4 "ambient0"J% +10 "core" ʂ%o +4 #"FILTERING"Pressurized cartridge with 0.526ml to 44.7psi +2 #Isolated @23.2psia (7.9psi) +5 #Verifying cartridge holds at least 0.65..0.85 of 23.2psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"J% +8 "core" ʄ%y +42 "ambient0"J% +5 "core" ʅ%rw +45 "ambient0"J% +4 "core" ʇ%m| +46 "ambient0"J % +4 "core" ʉ%pk +46 "ambient0"J\ % +3 "core" ʊ%tl +47 "ambient0"J % +4 "core" ʋ%i| +46 "ambient0"J % +6 "core" ʍ%mn +44 "ambient0"J% +4 "core" ʏ%} +46 "ambient0"J% +4 "core" ʑ%+ +46 "ambient0"J% +4 "core" ʓ% +3 "carousel0"F% +4 "core" ƕ%S^df \  +3 #"FILTERING"Sampling 100.0ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +8 ."FILTERING"Exhaust.open +2 "sampler0"H2 +150 "core" Ȝ2 +2 ."FILTERING"Intake.open +2 "sampler0"H1 +146 "core" ț1 +5 "Can"iU.zsnJb< \\ +2 "ambient0"J% +5 "core" ʚ%V +4 "ambient0"J% +4 "core" ʗ%a +3 ."FILTERING"Abs.in :default +2 "ambient0"J>:9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J+ +8 "core" ʖ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +7 "core" ǘ, +2 "ambient0"J% +6 "core" ʙ%@ +3 "sampler0"H% +4 "core" ȝ%b  +3 "sampler0"H#% +4 "core" ȣ%q  +8 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n#(L@?@3 22Q +8 ."FILTERING"SamplePump.seek 100ml,6:04 +3 "sampler0"H%'# +55 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d n#(L@?@3 22Q +229 H&% +3 "core" Ȧ%\ 3 $ ~2 +3 "ambient0"J'% +4 "core" ʧ%, } +2 #"sampling"Cartridge 20 filtering 2/100ml at 0.72ml/s with 9.3psi vacuum 5.3m deep (22.6psia) +1772 "Can"hU.zsRU +4222 "sampler0"H(% +3 "core" Ȩ%\ \  2 +3 "ambient0"J)% +4 "core" ʩ%b  +2 #"sampling"Cartridge 20 filtering 39/100ml at 0.63ml/s with 7.4psi vacuum 4.9m deep (21.9psia) +5987 "sampler0"H+% +3 "core" ȫ%j 2 +3 "ambient0"J,% +4 "core" ʬ%h +2 #"sampling"Cartridge 20 filtering 72/100ml at 0.55ml/s with 12.5psi vacuum +1723 "Can"iU/z s^T  +4049 "core" ȥ' +2 "sampler0"H% +4 "core" Ȇ%# 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."closeIntake"Intake.close +4 "sampler0"H/ +6 ."closeExhaust"Exhaust.close +5 "carousel0"F% +3 "sampler0"H0 +10 "core" Ɛ%S^d \  +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"D ( +104 "core" Ȏ/ +20 Ȓ0 +230 Č( +4 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"D( +354 "core" Ĉ( +3 #"FILTERING"Sampled 100.0ml +3 "carousel0"F$% +8 "core" Ƥ%S^e \  +7 "valve0"D.% +4 "core" Į%ZGO_ G +3 "syringe1"C0& +4 "core" ð&^ n +3 "syringe1"C % +4 "core" à%@ +3 "carousel0"F2% +4 "core" Ʋ%S]g? \  +4 "carousel0"F% +6 "core" ƃ%S^e \  +116 ."PROCESSING"SPR.command "read_sequences" +29 .SPR.command "stats_on" +9 "carousel0"F% +3 "core" Ɵ%S^g \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26jul3119h.spr.bz2 +30 ."sourcePumpShutdown"Gate.power :sampler,:OFF +222 ."PROCESSING"Handoff.select :waste +7 "valve0"D +105 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C3% +6 "core" ó%( +45 "syringe1"C4% +3 "core" ô%4 +47 "syringe1"C5% +4 "core" õ%B +46 "syringe1"C6% +4 "core" ö%L +46 "syringe1"C7% +3 "core" ÷%H +47 "syringe1"C8% +3 "core" ø%G +47 "syringe1"C9% +3 "core" ù%P +49 "syringe1"C:% +6 "core" ú%N +44 "syringe1"C;% +3 "core" û%: +47 "syringe1"C<% +3 "core" ü%4 +47 "syringe1"C=% +3 "core" ý%= +47 "syringe1"C>% +3 "core" þ% +47 "syringe1"C?% +4 "core" ÿ% +46 "syringe1"C@% +3 "core" % +6 "Can"iU/z t)h. ? + #"PROCESSING"Syringe tare is 50% of full scale at 29C (drifted -0%) +5 .PinPort.in :locking +3 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"EA( N +81 "core" ( +4 ."posArchive"Archive.in :fast +7 ."posLyS2"Lysis2.in :fast +6 ."PROCESSING"Lysis1.seek 1250uL +7 "syringe0"B?d PZ<2 +2 B>d P\\k<2 +4 "syringe1"CB( +8 ."posLyS2"Lysis2.seek 950uL +6 ."posArchive"Archive.seek :empty +7 "syringe0"BC' +6 BD( +232 "core" ( +265 ' +335 ( +2 "carousel0"FE% +4 "core" %S[| \  +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +4 .PinPort.seek :locked +5 "valve1"EF( N +5 "core" ( +5 ."PROCESSING"Handoff.select :waste +6 .Plunge.seek :puckBottom +7 "valve0"DG'B +127 "core" ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CH% +8 "core" %|T +43 "syringe1"CI% +3 "core" %Z +47 "syringe1"CJ% +3 "core" %Q +47 "syringe1"CL% +4 "core" %B +46 "syringe1"CN% +3 "core" %* +47 "syringe1"CO% +3 "core" %, +47 "syringe1"CP% +3 "core" %|3 +47 "syringe1"C1% +4 "core" ñ%}. +47 "syringe1"CQ% +3 "core" %{8 +47 "syringe1"CR% +3 "core" %yI +47 "syringe1"CS% +3 "core" %}S +47 "syringe1"CT% +3 "core" %X +47 "syringe1"CU% +3 "core" %}W +47 "syringe1"CV% +3 "core" %}U +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi +4 #Air stroke #1 of 1 +5 "syringe1"CW% +6 "core" %y[ +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +8 ."PROCESSING"Air.seek :pressurized +3 "syringe1"CX' +517 "core" ' +3 "syringe1"CY% +4 "core" %S2 < +2 #"PROCESSING"0.1psi after pressurize +3 .Air.in :compress +2 "syringe1"C>N  P]zP2 +8 ."PROCESSING"Air.seek :evac +2 "syringe1"CZ'| +653 "core" ' +3 "syringe1"C]% +5 "core" %|4d kPn +2 #"PROCESSING"1.8psi after compress +2 "syringe1"C^% +8 "core" %|4 j +72 "syringe1"CK% +4 "core" %|4h< e +16 "syringe1"C`% +4 "core" %|4i j +46 "syringe1"Ca% +4 "core" %|4hs p +46 "syringe1"C[% +4 "core" %|4h m +46 "syringe1"C\\% +4 "core" %|4aY _ +46 "syringe1"Cb% +4 "core" %|4i o +46 "syringe1"Cc% +4 "core" %|4d= h +46 "syringe1"Cd% +4 "core" %|4b( i +46 "syringe1"Cg% +3 "core" %|4g a +47 "syringe1"Ch% +4 "core" %|4` n +46 "syringe1"Ci% +4 "core" %|4\\ e +46 "syringe1"C_% +5 "core" %|4a c +45 "syringe1"Ck% +4 "core" %|4 e +48 "syringe1"Cl% +3 "core" %|4 q +45 "syringe1"Ce% +4 "core" %|4_ \\ +47 "syringe1"Cf% +3 "core" %|4b i +47 "syringe1"Cm% +3 "core" %|4_ _ +47 "syringe1"Cn% +3 "core" %|4_ X +47 "syringe1"C% +3 "core" Þ%|4` d +47 "syringe1"Co% +4 "core" %|4` f +46 "syringe1"Cp% +3 "core" %|4_N j +47 "syringe1"Cr% +3 "core" %|4xK d +47 "syringe1"Cs% +3 "core" %|4]P R +47 "syringe1"Ct% +3 "core" %|4ZH T +47 "syringe1"Cu% +3 "core" %|4WH f +47 "syringe1"Cv% +3 "core" %|4WJ e +47 "syringe1"Cw% +3 "core" %|4[W R +47 "syringe1"Cx% +3 "core" %|4_f e +3 #"PROCESSING"0.3psi after compress (stable) +2 .Air.in :default +9 "syringe1"C>d P]z<2 +7 ."PROCESSING"Air.hold +3 "syringe1"Cy+ +4 "core" + +4 "syringe1"C|% +9 "core" %|4l_ j +3 "syringe1"C}% +5 "core" %|4Wo [ +3 #"PROCESSING"at 0.4psi Opened to waste +4 .Handoff.select :blocked +4 "valve0"D0 +49 ."PROCESSING"Plunge.seek :puckTop +3 "valve0"Dj': +86 "core" ' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi + #Air stroke #1 of 8 +2 "syringe1"C% +9 "core" %|4Ot ] +3 ."PROCESSING"Air.in :compress +2 "syringe1"C>N  P]zP2 +6 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +7 "core" Á' +3 "syringe1"C% +8 "core" Â%|4 ^ +2 #"PROCESSING"1.9psi after compress +2 "syringe1"C% +9 "core" Ä%|4T e +41 "syringe1"C% +4 "core" Å%|4W f +46 "syringe1"C% +4 "core" Ç%|4X{ g +46 "syringe1"Cz% +4 "core" %|4 r +46 "syringe1"C{% +4 "core" %|4Rw [ +46 "syringe1"C % +4 "core" É%|4Q~ ^ +46 "syringe1"C\ % +4 "core" Ê%|4 a +46 "syringe1"C % +4 "core" Ë%|4z Y +46 "syringe1"C % +5 "core" Í%|4 ] +45 "syringe1"C% +4 "core" Ï%|4 T +46 "syringe1"C% +4 "core" Ñ%|4 R +46 "syringe1"C% +4 "core" Ó%|4 d +46 "syringe1"C% +3 "core" Õ%|4 _ +47 "syringe1"C% +4 "core" Ü%|4 Y +46 "syringe1"C% +3 "core" Û%|4 f +48 "syringe1"C% +6 "core" Ú%|4 d +58 "syringe1"C% +3 "core" ×%|4 b +34 "syringe1"C% +4 "core" Ù%|4 U +46 "syringe1"C% +4 "core" Ý%|4 ^ +46 "syringe1"C#% +4 "core" ã%|4 g +46 "syringe1"C&% +3 "core" æ%|4} b +47 "syringe1"C'% +3 "core" ç%|4 ] +3 #"PROCESSING"2.1psi after compress (stable) +3 .Air.in :gulp +4 "syringe1"C>N  P]z<2 +6 ."PROCESSING"Air.seek :topVent +3 "syringe1"C~'6 +6 "Can"kU/ztD +634 "core" ' +3 "syringe1"C(% +4 "core" è%6: < +2 #"PROCESSING"2.0psi after gulp +3 "syringe1"C)% +8 "core" é%6t{ +42 "syringe1"C+% +3 "core" ë%6 u +47 "syringe1"C,% +3 "core" ì%6n +47 "syringe1"C% +4 "core" Æ%6t +46 "syringe1"C% +3 "core" Ð%6t +47 "syringe1"C% +6 "core" Î%6t +45 "syringe1"C% +3 "core" Ò%6 +47 "syringe1"C% +4 "core" Ö%6n +46 "syringe1"C% +4 "core" Ø%6p +46 "syringe1"Cq% +4 "core" %6u +46 "syringe1"C % +5 "core" Ì%6p +45 "syringe1"C$% +3 "core" ä%6m +47 "syringe1"C.% +3 "core" î%6s +3 #"PROCESSING"2.2psi after gulp (stable) + #Air stroke #2 of 8 +4 "syringe1"C0% +7 "core" ð%6s +4 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +7 ."PROCESSING"Air.seek :pressurized +4 "syringe1"C ' +631 "core" à' +3 "syringe1"C2% +4 "core" ò%:S < +2 #"PROCESSING"2.3psi after pressurize +3 .Air.in :compress +2 "syringe1"C>N  P]zP2 +8 ."PROCESSING"Air.seek :evac +3 "syringe1"C'| +654 "core" Ã' +2 "syringe1"C% +11 "core" ß%| 4x fP| +2 #"PROCESSING"13.4psi after compress +3 "syringe1"C3% +8 "core" ó%|4ra y +42 "syringe1"C4% +3 "core" ô%|4_ w +47 "syringe1"C5% +3 "core" õ%|4_ x +47 "syringe1"C6% +4 "core" ö%|4d e +46 "syringe1"C7% +3 "core" ÷%|4` t +47 "syringe1"C8% +3 "core" ø%|4Z n +47 "syringe1"C9% +3 "core" ù%|4[; u +47 "syringe1"C:% +3 "core" ú%|4[O p +47 "syringe1"C;% +3 "core" û%|4Ui m +47 "syringe1"C<% +3 "core" ü%|4Yi l +47 "syringe1"C=% +4 "core" ý%|4V e +46 "syringe1"C>% +3 "core" þ%|4R g +47 "syringe1"C?% +3 "core" ÿ%|4Y g +47 "syringe1"C@% +6 "core" %|4S f +44 "syringe1"C% +3 "core" Ô%|4V h +48 "syringe1"C-% +3 "core" í%|4[ j +48 "syringe1"C!% +3 "core" á%|4X c +47 "syringe1"C"% +45 "core" â%|4U k +5 "syringe1"CE% +3 "core" %|4W f +47 "syringe1"CH% +3 "core" %|4P \\ +47 "syringe1"CI% +4 "core" %|4V h +46 "syringe1"CJ% +4 "core" %|4 _ +46 "syringe1"CA% +3 "core" %|4 i +47 "syringe1"CL% +3 "core" %|4 ^ +47 "syringe1"C/% +3 "core" ï%|4 g +47 "syringe1"CN% +3 "core" %|4 m +47 "syringe1"CO% +4 "core" %|4 i +2 #"PROCESSING"16.3psi after compress (stable) +3 .Air.in :gulp +7 "syringe1"C>N  P]z<2 +7 ."PROCESSING"Air.seek :topVent +3 "syringe1"CP'6 +638 "core" ' +2 "syringe1"C1% +5 "core" ñ%6L < +2 #"PROCESSING"16.2psi after gulp +7 "syringe1"CQ% +6 "core" %6uM +43 "syringe1"CR% +3 "core" %6x z +47 "syringe1"CS% +3 "core" %6y +47 "syringe1"CT% +4 "core" %6u +46 "syringe1"CU% +3 "core" %6{ +47 "syringe1"CV% +3 "core" %6x } +48 "syringe1"CW% +4 "core" %6{ } +46 "syringe1"CM% +3 "core" %6x x +47 "syringe1"CY% +3 "core" %6v +47 "syringe1"CX% +3 "core" %6u  +47 "syringe1"C]% +4 "core" %6u +46 "syringe1"C^% +3 "core" %6 +3 #"PROCESSING"16.3psi after gulp (stable) + #Air stroke #3 of 8 +4 "syringe1"CK% +10 "core" %6u y +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +7 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C`' +629 "core" ' +3 "syringe1"Ca% +4 "core" %DS < +3 #"PROCESSING"15.9psi after pressurize +5 .Air.in :compress +5 "syringe1"C>N  P]zP2 +8 ."PROCESSING"Air.seek :evac +5 "syringe1"C['| +472 "core" '7p=B fP +5 "syringe1"C\\% +5 "core" %7=B mP +3 #"PROCESSING"Reached 20.4 of 20.0psi after pumping 5223uL (2.94 strokes) +2 .Air.in :default +4 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.hold +3 "syringe1"Cb+ +4 "core" + +4 ."PROCESSING"Plunge.seek :puckBottom +4 "valve0"Dc'B +84 "core" ' +3 ."PROCESSING"delay 3 seconds +302 .Handoff.select :waste +2 "valve0"D +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cd% +6 "core" %7=C_ +44 "syringe1"Cg% +4 "core" %7=G^ +46 "syringe1"Ch% +3 "core" %7=Jb +47 "syringe1"Ci% +5 "core" %7=B]\\ +45 "syringe1"C_% +3 "core" %7=Ga? +48 "syringe1"Ck% +6 "core" %7==` +43 "syringe1"Cl% +3 "core" %7=C^ +47 "syringe1"Ce% +4 "core" %7=P_ | +47 "syringe1"Cf% +3 "core" %7=F[ +47 "syringe1"Cm% +45 "core" %7=Q`^ { +5 "syringe1"Cn% +3 "core" %7=S^{ y +47 "syringe1"C% +5 "core" Þ%7=H\\V +45 "syringe1"Co% +3 "core" %7=KZS +47 "syringe1"Cp% +3 "core" %7=NZV ~ +47 "syringe1"Cr% +3 "core" %7=UU w +47 "syringe1"Cs% +3 "core" %7=KW +47 "syringe1"Ct% +3 "core" %7=MUy  +47 "syringe1"Cu% +3 "core" %7=HUy +47 "syringe1"Cv% +3 "core" %7=K~ +47 "syringe1"Cw% +3 "core" %7=HTt +47 "syringe1"Cx% +3 "core" %7=OP } +47 "syringe1"C|% +5 "core" %7=UVm w +3 ."PROCESSING"delay 1:00 +4102 "Can"mUL/zt> +1900 ."PROCESSING"Handoff.select :blocked +6 "valve0"D0 +56 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi +2 "syringe1"C}% +6 "core" %7=ZMX r +45 "syringe1"C% +3 "core" %7=]SP o +47 "syringe1"C% +4 "core" Â%7=eQ[ g +46 "syringe1"C% +5 "core" Ä%7=`V l +45 "syringe1"C% +3 "core" Å%7=cL i +47 "syringe1"CZ% +3 "core" %7=eO g +47 "syringe1"C% +3 "core" Ç%7=b; j +47 "syringe1"Cz% +3 "core" %7=[5 q +47 "syringe1"CG% +3 "core" %7=`3 l +47 "syringe1"C{% +4 "core" %7=d( h +46 "syringe1"C % +6 "core" É%7=eP g +44 "syringe1"Cy% +3 "core" %7=^b n +48 "syringe1"C\ % +3 "core" Ê%7=Wk u +46 "syringe1"C % +3 "core" Ë%7=dp h +47 "syringe1"C % +3 "core" Í%7=_l m +90 "syringe1"C% +6 "core" Ï%7=cn i +3 "syringe1"C% +15 "core" Ñ%7=S^ y +33 "syringe1"C% +3 "core" Ó%7=^` n +47 "syringe1"C% +3 "core" Õ%7=_S m +47 "syringe1"C% +5 "core" Ü%7=]E o +45 "syringe1"C% +3 "core" Û%7=[; q +47 "syringe1"C% +3 "core" Ú%7=`B l +47 "syringe1"C% +3 "core" ×%7=]M o +47 "syringe1"C% +3 "core" Ù%7=eb g +47 "syringe1"C% +3 "core" Ý%7=[t q +47 "syringe1"C#% +3 "core" ã%7=Vs v +47 "syringe1"C&% +3 "core" æ%7=ck i +47 "syringe1"C'% +4 "core" ç%7=et g +46 "syringe1"C% +3 "core" Á%7=fu f +48 "syringe1"C(% +4 "core" è%7=dg h +45 "syringe1"C)% +4 "core" é%7=_d m +46 "syringe1"C+% +4 "core" ë%7=[W q +46 "syringe1"C,% +3 "core" ì%7=cA i +47 "syringe1"C% +3 "core" Æ%7=SB y +47 "syringe1"C% +3 "core" Ð%7=Z~> r +47 "syringe1"C% +3 "core" Î%7=_> m +47 "syringe1"C% +3 "core" Ò%7=]< o +47 "syringe1"C% +3 "core" Ö%7=Z` r +47 "syringe1"C% +3 "core" Ø%7=[}r q +47 "syringe1"Cq% +3 "core" %7=Sk y +48 "syringe1"C % +3 "core" Ì%7=Wf u +46 "syringe1"C$% +4 "core" ä%7=W~u u +47 "syringe1"C.% +3 "core" î%7=\\f p +47 "syringe1"C0% +3 "core" ð%7=[ym q +47 "syringe1"C~% +4 "core" %7=]U o +45 "syringe1"C2% +4 "core" ò%7=bL j +2 ."PROCESSING"Handoff.select :waste +3 "valve0"D +48 #"PROCESSING"0.3psi at evacEnd +4 .Plunge.seek :pTop +9 "valve0"D ': +83 "core" Ġ' +2 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +3 "syringe1"C>d  P]z<2 +10 ."PROCESSING"Air.seek :fullVent +2 "syringe1"C'݇ +269 "core" ß' +2 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :pBottom +3 "valve0"D3'B +85 "core" ij' + #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +3 .Plunge.seek :locked +5 "valve0"D4'Z +129 "core" Ĵ' +3 ."PROCESSING"Handoff.select :waste +5 "syringe1"C5& +7 "core" õ&f\\ +4 ."PROCESSING"Lysis1.in :slow +2 "syringe1"C?d P[<2 +6 ."PROCESSING"Lysis1.seek 950uL +3 "syringe1"C6( +1406 "core" ö( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select :blocked +47 "valve0"D0 +52 ."PROCESSING"Plunge.seek :puckTop +3 "valve0"D7': +87 "core" ķ' +2 "syringe1"C8& +4 "core" ø&{x;  +4 ."PROCESSING"Lysis1.seek 350uL +3 "syringe1"C9( +81 "core" ù(bjzÁ+ < +8 "syringe1"C:& +4 "core" ú&^\ zā% < +3 ."PROCESSING"Lysis1.setRawPosition! -4926 +2 "syringe1"C8 +7 !"PROCESSING"o:Slide::Error : mesg"@Lysis Syringe #1 (LyS1) positionErr at 935uL (sensed 958uL):@timestampu: Time   : @replyo:I2C::Servo::AbsMove3Reply : @error:positionErr:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmiA:@deltaPressi+:@pastThresholdF: @voltagef14.695173294059893e: @currenti: @type2F:@outPressi:\ @lostT: @inPressiz:@velocityij: @pastFLSF:\ @homeF: @enabledF: @erri:@positionib: @pastRLSF: @tagi>: @sourceiH:@checkpoint: omitted:@axisName"Lysis Syringe #1 (LyS1):bt["2/home/esp/esp2/lib/slide.rb:652:in `raiseErr'"7/home/esp/esp2/lib/slide.rb:413:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'"./home/esp/esp2/lib/slide.rb:370:in `seek'"0/home/esp/esp2/lib/slide.rb:420:in `moveTo'"./home/esp/esp2/lib/scale.rb:204:in `push'"H/home/esp/esp2/protocol/3G/function/LyseGo.rb:168:in `deliverLysis'"+/home/esp/esp2/lib/slide.rb:94:in `in'"H/home/esp/esp2/protocol/3G/function/LyseGo.rb:163:in `deliverLysis'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:382:in `lyse'"B/home/esp/esp2/protocol/3G/function/LyseGo.rb:417:in `lyseDA'"?/home/esp/esp2/protocol/3G/function/spr.rb:228:in `runSPR'">/home/esp/esp2/protocol/3G/function/spr.rb:204:in `doSPR'"?/home/esp/esp2/protocol/3G/function/spr.rb:221:in `runSPR'"@/home/esp/esp2/protocol/3G/function/spr.rb:40:in `microSPR'"?/home/esp/esp2/protocol/3G/common3Gcfg.rb:19:in `microSPR'";/home/esp/esp2/utils/cartridge/common.rb:384:in `call'">/home/esp/esp2/utils/cartridge/common.rb:384:in `process'">/home/esp/esp2/utils/auv/cmd.rb:488:in `startProcessing!'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 #Waiting to Resume from Checkpoint "Lysis Syringe #1 (LyS1) positionErr at 935uL (sensed 958uL)" +4120 "Can"lU/ztF>/ +11956 jU/ztE|M +11958 jVk.ztF'ok +11957 lV.z tHb +11955 mV.z tC9T +8613 #"SPRlogger"Ended log spr26jul3119h.spr.bz2 +2 ."stopSPRafterError"SPR.command "stats_off" +3342 "Can"lW/z\ tEwH +11955 lW/ztCl< +11957 jWp/ztG \ 0 +11958 jW/ztC! $ +11957 mW/ztCC= +11960 kW/ztI[ +11959 mW/ztCny +11961 kW/ztFj +11961 kW/ztCL +11957 mX+/ztD. +11956 kX/ztDY! +11958 mX9/ztE$ +11957 mXQ/z tB{'- +11959 mXq/ztH2*K +11953 mX}/ztE -i +11952 mX/ztD0 +11952 lX/ ztD3 +11953 mX/\ ztAU6 +11952 kX/ ztI;9 +11954 mX/ ztDm< +11956 lY_/ ztBj? +11956 kYh/ztCB; +11957 lY/ztCwEY +11957 jY/ztDUHyw +11953 mY/ztB/Kt +11953 mY/ztC\\Nn +11955 nY/z tEQh +11956 kZ/ztAyTc +11957 mZ/ztE+W_ +11957 mZb/z\ tCJZZ+ +11961 kZ/ytCg]UI +11960 mZU/zt?`Qg +11961 nZk/ztEcM +11960 kZ/ztAfI +11956 mZ/ztCiE +11958 m[/ztE4lA +11958 mZ/ztDho= +11959 l[=/ztBr9 +11956 m[/ztAu69 +11955 m[/z tBx2W +11955 o[/ztA{/u +11955 m[0/ztEo~- +11956 l[ /ztAj+ +11956 m[o.ztCn( +11956 l[.ztB~& +11959 k[.ztB# +11952 l[/z tA!) +11955 m[.ztAG +11957 n[*.z\ tDe +11955 k[X/ztCL +11954 m[.ztBs +11958 n[.z tB +11957 k[/ztE +11955 o[.ztB +11956 l[.ztD +11956 m[.ztC7 +11953 n[.ztDU +11955 k[/ztETs +11955 n[.ztCa +11954 m[.ztC +11953 k\\(/ztA +11957 k\\/ztB +11957 l\\~.ztA +11953 m\\?.ztDm' +11956 j\\8.ztCqE +11955 l\\*.ztFDc +11959 n\\/.ztCr +11961 l\\.ztAD +11956 n\\,.ztA +11959 m\\.ztC +11956 k\\.ztE +6754 #"preston#2"Session Begun +820 #-> Lysis1 +3 "syringe1"C;& +7 "core" û&zvy +1782 #"preston#2"-> Lysis1.home.jog 0 +4 .Lysis1.in :default +6 "syringe1"C?d P[<2 +6 ."preston#2"Lysis1.home.jog 0 +3 "syringe1"C<* +12 "core" ü* +3 "syringe1"C=& +3 "core" ý&zd \ +1171 #"preston#2"-> Handoff.blocked +3 .Handoff.select :blocked +1378 "Can"k\\t.ztC +1059 #"preston#2"-> Lysis1.to 350, :slow +6 .Lysis1.in :slow +3 "syringe1"C?d P[<2 +8 ."preston#2"Lysis1.seek 350uL +3 "syringe1"C>( +2837 "core" þ( +2 "syringe1"C?& +4 "core" ÿ&G < +8035 "Can"n\\.ztA 5 +1265 #"preston#2"-> Air.in :slow do +3 !o:SyntaxError: mesg"^compile error\ preston#2:5: syntax error, unexpected $end\ Air.in :slow do\ ^:@timestampu: Time "4:bt["%preston#2:5:in `topLevelBinding'" Syringe.mix Air=>Air.volume-Air.at(:evac).volume, +5 !o:SyntaxError: mesg"compile error\ preston#2:6: syntax error, unexpected $end\ Syringe.mix Air=>Air.volume-Air.at(:evac).volume,\ ^:@timestampu: Time "8c:bt["%preston#2:6:in `topLevelBinding'" syr=>160 #helps seat check valve +6 !o:SyntaxError: mesg"wcompile error\ preston#2:7: syntax error, unexpected tASSOC, expecting $end\ syr=>160 #helps seat check valve\ ^:@timestampu: Time "`g:bt["%preston#2:7:in `topLevelBinding'" end +3 !o:SyntaxError: mesg"=compile error\ preston#2:8: syntax error, unexpected kEND:@timestampu: Time ".k:bt["%preston#2:8:in `topLevelBinding'" Air.reconfigure :slow +5 .Air.in :slow +2 "syringe1"C>d P]z<2 +8 C@% +3 "core" %݇/4Y +4816 #"preston#2"-> Syringe.mix Air=>Air.volume-Air.at(:evac).volume, Lysis1=>160 +3 "syringe1"C% +7 "core" Ô%݇/ +5 ."preston#2"Air.in mix with 2.79934 parts Lysis1 + "syringe1"C> wk+ P]z<2 +8 C-% +3 "core" í%݇/Hv z +3 ."preston#2"Lysis1.in :default +2 "syringe1"C?d P[<2 +9 C!& +2 "core" á&GG +57 ."Airmixer"Air.seek :evac +6 ."preston#2"Lysis1.seek 190uL +8 "syringe1"C"'| +4 CE( +186 "core" ( +573 â' +3 "syringe0"BH& +5 "core" &$* 遃 +2 "syringe1"CI% +5 "core" %|&4F; < +2 "syringe1"CJ& +5 "core" &9wA O +2 "syringe0"BA% +5 "core" %{z z +1190 #"preston#2"-> Air.fill :fast +7 .Air.in :fast +2 "syringe1"C> P]z<2 +7 ."preston#2"Air.seek :full +6 "syringe1"CL'6 +646 "core" ' +2 "syringe1"C/% +4 "core" ï%63 < +398 #"preston#2"-> Lysis1.empty +4 .Lysis1.seek :empty +6 "syringe1"CN( +212 "core" ( +3 "syringe1"CO& +5 "core" &) < +66 "Can"l\\o.ztA߬S +985 #"preston#2"-> "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % +4 !o:SyntaxError: mesg"{compile error\ preston#2:13: syntax error, unexpected $end\ ...} Syringe pressure @%.1fpsi" %\ ^:@timestampu: Time "I뒜:bt["&preston#2:13:in `topLevelBinding'" "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % Syringe.pressure +3 "valve0"D% +7 "core" ă%:7}OT 9 +3 "syringe1"C1% +5 "core" ñ%6 +974 #"preston#2"-> Plunge.pBottom +6 .Plunge.seek :pBottom +5 "valve0"DQ'B +85 "core" ' +2 "valve0"DR% +5 "core" %B(*Xm - +1353 #"preston#2"-> "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % Syringe.pressure +3 "valve0"DS% +7 "core" %B("On +3 "syringe1"CT% +4 "core" %6f +6869 "Can"n\\o.ztBq +5872 #"preston#2"-> padProfile +5 #Polling Can every 2:00 +54 "Can"n\\.ztC0 +2 ."preston#2"PuckHeater.configure :default #ambient 28.7C +2 "Cartridge" \ :D H  +8 ."preston#2"PuckHeater.seek 81.3C +7 "Cartridge" U  +4 V9 +6 "cartridge" 9& +107 "Cartridge" W9 +3 "cartridge" 9&_d +104 "Cartridge" M9 +4 "cartridge" 9'id +103 "Cartridge" Y9 +4 "cartridge" 9* d +104 "Cartridge" X9 +3 "cartridge" 9.d +103 "Cartridge" ]9 +3 "cartridge" 92Gd +104 "Cartridge" ^9 +3 "cartridge" 95d +104 "Cartridge" K9 +3 "cartridge" 98d +104 "Cartridge" P9 +3 "cartridge" 9;d +103 "Cartridge" a9 +4 "cartridge" 9>#d +104 "Cartridge" `9 +4 "cartridge" 9@c'd +103 "Cartridge" \\9 +3 "cartridge" 9Bj,d +104 "Cartridge" [9 +3 "cartridge" 9DS0d +104 "Cartridge" j9 +3 "cartridge" 9F14d +103 "Cartridge" d9 +5 "cartridge" 9G9d +104 "Cartridge" g9 +5 "cartridge" 9I=d +104 "Cartridge" h9 +3 "cartridge" 9KcAd +103 "Cartridge" i9 +4 "cartridge" 9LFd +104 "Cartridge" _9 +3 "cartridge" 9N@Jd +103 "Cartridge" k9 +4 "cartridge" 9ONd +103 "Cartridge" l9 +4 "cartridge" 9Q Rd +103 "Cartridge" e9 +3 "cartridge" 9RWd +104 "Cartridge" f9 +3 "cartridge" 9S[d +104 "Cartridge" m9 +3 "cartridge" 9UF_d +104 "Cartridge" n9 +4 "cartridge" 9V|Fdd +103 "Cartridge" 9 +3 "cartridge" 9Whd +104 "Cartridge" o9 +3 "cartridge" 9Xld +103 "Cartridge" p9 +4 "cartridge" 9Z2Fqd +103 "Cartridge" r9 +3 "cartridge" 9[[Fud +104 "Cartridge" s9 +4 "cartridge" 9\\Fyd +104 "Cartridge" t9 +3 "cartridge" 9]F}d +103 "Cartridge" u9 +4 "cartridge" 9^d +103 "Cartridge" v9 +3 "cartridge" 9_rd +104 "Cartridge" w9 +3 "cartridge" 9ad +104 "Cartridge" x9 +3 "cartridge" 9bd +103 "Cartridge" |9 +3 "cartridge" 9cd +104 "Cartridge" }9 +3 "cartridge" 9dc +103 "Cartridge" 9 +4 "cartridge" 9ed +103 "Cartridge" 9 +3 "cartridge" 9f d +104 "Cartridge" 9 +4 "cartridge" 9gc +103 "Cartridge" 9 +3 "cartridge" 9hd +104 "Cartridge" Z9 +3 "cartridge" 9hd +104 "Cartridge" 9 +3 "cartridge" 9jd +103 "Cartridge" z9 +4 "cartridge" 9jd +106 "Cartridge" G9 +5 "cartridge" 9kd +103 "Cartridge" {9 +3 "cartridge" 9l#d +104 "Cartridge" 9 +3 "cartridge" 9m#d +104 "Cartridge" y9 +3 "cartridge" 9nr#d +104 "Cartridge" \ 9 +3 "cartridge" 9oz#d +103 "Cartridge" 9 +4 "cartridge" 9pROd +148 "Cartridge" 9 +3 "cartridge" 9qOd +104 "Cartridge" 9 +3 "cartridge" 9riOd +103 "Cartridge" 9 +4 "cartridge" 9sjOd +103 "Cartridge" 9 +4 "cartridge" 9t@|d +103 "Cartridge" 9 +3 "cartridge" 9t|d +104 "Cartridge" 9 +3 "cartridge" 9u|d +62 "Can"k\\G.zshP +42 "Cartridge" 9 +3 "cartridge" 9vd +103 "Cartridge" 9 +4 "cartridge" 9w|c +103 "Cartridge" 9 +3 "cartridge" 9xjd +104 "Cartridge" 9 +4 "cartridge" 9yGd +103 "Cartridge" 9 +3 "cartridge" 9z.c +104 "Cartridge" #9 +3 "cartridge" 9zd +103 "Cartridge" &9 +4 "cartridge" 9{ d +103 "Cartridge" '9 +4 "cartridge" 9| d +103 "Cartridge" 9 +4 "cartridge" 9}\\d +104 "Cartridge" (9 +3 "cartridge" 9~)d +106 "Cartridge" )9 +4 "cartridge" 9~d +103 "Cartridge" +9 +3 "cartridge" 9,d +104 "Cartridge" ,9 +3 "cartridge" 9,#c +103 "Cartridge" 9 +4 "cartridge" 9J'b +103 "Cartridge" 9 +4 "cartridge" 9+a +103 "Cartridge" 9 +3 "cartridge" 90_ +104 "Cartridge" 9 +3 "cartridge" 9[,4] +14 ."preston#2"PuckHeater.hold 2:00,81.3C +9 "Cartridge"  G) +4 "cartridge" 5 +10174 "Can"n\\.zs- +3 n\\.zs-q +1749 "cartridge" +3 #"preston#2"Polling Can every 2:00 +53 "Can"l\\/ztDzQ +3 ."preston#2"PuckHeater.configure :default #ambient 28.8C +3 "Cartridge" \ :D H  +6 ."preston#2"PuckHeater.seek 80.2C +4 "Cartridge"   +5 q9 +7 "cartridge" 9 +5 ."preston#2"PuckHeater.hold 2:00,80.2C +7 "Cartridge" nQ +9 "cartridge"  +10102 "Can"j\\{.zt\ (L +4 j\\{.zs( +1813 "cartridge" +3 #"preston#2"Polling Can every 2:00 +53 "Can"j\\u.ztCJz +3 ."preston#2"PuckHeater.configure :default #ambient 28.8C +2 "Cartridge" \ :D H  +7 ."preston#2"PuckHeater.hold 8:00,79.2C +10 "Cartridge" $ \ { +10067 "Can"l\\p/zt'  +3 l\\p/zt 'W +11957 k\\/zt& +3 k\\/zt& +11957 k\\.zt&[*% +3 k\\.zt &+% +11955 l\\G.zt&CC +3 l\\G.zt&C +1762 "cartridge" +3 "Cartridge" .9 +4 "cartridge" 9a +1176 #"preston#2"-> Heat +3 "Cartridge" 09 +8 "cartridge" 9|d +675 #"preston#2"-> Power.status +968 #-> Heat.status +2 "Cartridge" ~9 +8 "cartridge" 9w +1183 #"preston#2"-> Log.comment "Lysate cooked @%.1fpsi" % Syringe.pressure; Plunge.to :puckTop; Air.pumpTo 40, 5; delay 2; Air.fill :fast; Plunge.to :puckBottom; Lysis1.pull 138, :slow; delay 2; Plunge.pTop; Lysis1.ignoreErrTo :empty; Plunge.to :puckBottom; Cart.sampler.recoverLysate +5 "syringe1"C2% +6 "core" ò%6Y z +2 #"preston#2"Lysate cooked @11.7psi +3 .Plunge.seek :puckTop +9 "valve0"Dc': +82 "core" ' +3 #"preston#2"Air syringe pumping up to 5 strokes to reach 40psi +2 #Air stroke #1 of 5 +5 "syringe1"Cb% +7 "core" %6I- +3 ."preston#2"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +7 ."preston#2"Air.seek :pressurized +3 "syringe1"C5' +631 "core" õ' +2 "syringe1"C % +4 "core" à%S܉\ < +3 #"preston#2"11.0psi after pressurize +3 .Air.in :compress +6 "syringe1"C>N  P]zP2 +6 ."preston#2"Air.seek :evac +3 "syringe1"C3'| +221 "core" ó'pI/ mP +4 "syringe1"CB% +4 "core" %I$ˌ0 tP +3 #"preston#2"Reached 15.4 of 40.0psi after pumping 1528uL (0.86 strokes) +2 .Air.in :slow +6 "syringe1"C>d P]z<2 +6 ."preston#2"Air.hold +3 "syringe1"C8+ +5 "core" ø+ +4 ."preston#2"delay 2 seconds +207 .Air.in :fast +2 "syringe1"C> P]z<2 +8 ."preston#2"Air.seek :full +3 "syringe1"C9'6 +562 "core" ù' +4 ."preston#2"Plunge.seek :puckBottom +3 "valve0"D:'B +86 "core" ĺ' +2 "syringe1"C4& +4 "core" ô&) +3 ."preston#2"Lysis1.in :slow +2 "syringe1"C?d P[<2 +9 ."preston#2"Lysis1.seek 138uL +2 "syringe1"C6(# +654 "core" ö( +2 ."preston#2"delay 2 seconds +204 .Plunge.seek :pTop +2 "valve0"D;': +86 "core" Ļ' +3 ."preston#2"Lysis1.in :default + "syringe1"C?d P[<2 +9 ."preston#2"Lysis1.seek :empty +3 "syringe1"C=( +167 "core" ý( +3 ."preston#2"Plunge.seek :puckBottom +3 "valve0"D<'B +86 "core" ļ' +3 "valve0"D?% +5 "core" Ŀ%B(Xv - +4 "syringe1"C@% +7 ."preston#2"Lysis1.in :extract +2 "syringe1"C?d 'J P[#2 +7 "core" %6~ +5 ."preston#2"Lysis1.seek 675uL,nil +3 "syringe1"C( +7 C-& +5 "core" í&*) # +4 "syringe1"C!& +4 "core" á& )ď # +6 "syringe1"C& +3 "core" ß&*:ˏ # +19 "syringe1"C>& +5 "core" þ&M ,!ƎS # +4 "syringe1"CH% +4 "core" %6 ȍ +5 "syringe1"CI& +3 "core" &ݞ -, # +17 "syringe1"CJ& +3 "core" &$ /‹ #s +17 "syringe1"CA& +3 "core" &b 1ȊG #d +52 "syringe1"C"% +3 "core" â%6ƈe +2 "syringe1"C/& +6 CO% +17 "core" ï&1\ 5Ĉ_ #{ + %6D +3 "syringe1"CE& +5 ."pump"Air.in :pressurize +4 "syringe1"C>@N I P]z<2 +7 "core" &65ĈN t +4 ."pump"Air.seek :pressurized +3 "syringe1"C& +2 C1' +9 "core" Ã&65z  +5 "syringe1"CR& +5 "core" &65X s +3 "syringe1"C7& +5 "core" ÷&6+5m t +8 "syringe1"CS& +3 "core" &6/5 } +16 "syringe1"CT& +4 "core" &6/5H w +16 "syringe1"CV& +3 "core" &675Ĉo p +17 "syringe1"CW& +3 "core" &665Ĉ\\ { +17 "syringe1"CM& +3 "core" &635Y y +17 "syringe1"CY& +4 "core" &615o k +16 "syringe1"CX& +3 "core" &6<5d y +17 "syringe1"C]& +3 "core" &6>5f l +17 "syringe1"C^& +3 "core" &6D5T t +17 "syringe1"CK& +3 "core" &6B5g r +17 "syringe1"CP& +3 "core" &675b q +17 "syringe1"Ca& +4 "core" &6?5h l +16 "syringe1"C`& +3 "core" &6C5a r +17 "syringe1"C\\& +3 "core" &6M5Z s +17 "syringe1"C[& +3 "core" &6R5c v +17 "syringe1"Cj& +4 "core" &6L5e d +16 "syringe1"Cd& +3 "core" &6G5B n +17 "syringe1"Cg& +3 "core" &6N5V | +17 "syringe1"Ch& +3 "core" &6P5W n +17 "syringe1"Ci& +3 "core" &6X5L h +17 "syringe1"C_& +3 "core" &6Z5B v +17 "syringe1"Ck& +3 "core" &6P5D u +17 "syringe1"Cl& +3 "core" &6W58 | +17 "syringe1"Ce& +3 "core" &6\\5E k +17 "syringe1"Cf& +3 "core" &6^5C p +17 "syringe1"Cm& +3 "core" &6^5^ t +17 "syringe1"Cn& +3 "core" &6U5\\ u +17 "syringe1"C& +3 "core" Þ&6[5b q +17 "syringe1"Co& +3 "core" &6Z5M o +17 "syringe1"Cp& +3 "core" &6f5X k +18 "syringe1"Cr& +2 "core" ñ' +5 "syringe1"Cs% +5 "core" &635X n + %1Si_ < +3 #"pump"10.1psi after pressurize +3 .Air.in :compressLysate +6 "syringe1"C>dN I P]z<2 +7 Ct& +2 ."pump"Air.seek :evac +6 "core" &65C l + "syringe1"Cu'| +9 Cv& +5 "core" &635k n +5 "syringe1"Cw& +3 "core" &6.5 t +16 "syringe1"Cx& +3 "core" &6A5ˆ f +17 "syringe1"C|& +3 "core" &6@5 o +17 "syringe1"C}& +4 "core" &6+5 { +16 "syringe1"C& +3 "core" &6K5) l +17 "syringe1"C& +3 "core" Â&625"% u +17 "syringe1"C& +3 "core" Ä&6A5[ w +17 "syringe1"C& +3 "core" Å&6P5q o +17 "syringe1"CZ& +3 "core" &6O5 n +17 "syringe1"C& +3 "core" Ç&6*5| _ +17 "syringe1"Cz& +3 "core" &6P5 s +17 "syringe1"CG& +3 "core" &6F5 k +17 "syringe1"C{& +3 "core" &615 y +17 "syringe1"C & +3 "core" É&6R5! o +17 "syringe1"Cy& +3 "core" &6T52 u +17 "syringe1"C\ & +3 "core" Ê&6N5G i +17 "syringe1"C & +3 "core" Ë&6Q5t n +17 "syringe1"C & +3 "core" Í&6W5 h +17 "syringe1"C& +3 "core" Ï&6M5 n +17 "syringe1"C& +3 "core" Ñ&6P5 w +17 "syringe1"C& +3 "core" Ó&655 m +17 "syringe1"C& +3 "core" Õ&6O58 h +17 "syringe1"C& +3 "core" Ü&6B5K r +17 "syringe1"C& +3 "core" Û&6@5n k +17 "syringe1"C& +3 "core" Ú&6]5 m +17 "syringe1"C& +3 "core" ×&6W5 q +17 "syringe1"C& +3 "core" Ù&65 #h +17 "syringe1"C& +3 "core" Ý&6D5 #m +17 "syringe1"C#& +4 "core" ã&8g5+ ~#o +16 "syringe1"C&& +3 "core" æ&=5?6  #d +17 "syringe1"C'& +3 "core" ç&EMc68& |#` +17 "syringe1"C& +3 "core" Á&Pfj6u7 #[ +17 "syringe1"C(& +3 "core" è&^l6ތ #Z +17 "syringe1"C)& +3 "core" é&nd7' |#R +17 "syringe1"C+& +3 "core" ë&߁q7j {#] +17 "syringe1"C,& +3 "core" ì&ߕn7 ~#Y +17 "syringe1"C& +3 "core" Æ&߫p8B #Y +17 "syringe1"C& +3 "core" Ð&U8 #d +17 "syringe1"C& +3 "core" Î&f9 #m +17 "syringe1"C& +3 "core" Ò&_9狌 ~#u +17 "syringe1"C& +3 "core" Ö& F:/i }#n +17 "syringe1"Cq& +3 "core" &5Az:H #y +17 "syringe1"C & +3 "core" Ì&`l; ~#k +17 "syringe1"CU& +3 "core" &r< ~# +17 "syringe1"C& +3 "core" Ø&et=C ~# +17 "syringe1"C$& +3 "core" ä&r>^ފ ~# +17 "syringe1"C.& +3 "core" î&Q>p y# +17 "syringe1"C0& +3 "core" ð&TZ? |# +17 "syringe1"C~& +3 "core" &Pr?8 ~# +17 "syringe1"C2& +50 "core" ò&qp@ }# +2 "syringe1"Cb& +4 "core" &zt@ |# +13 "syringe1"CQ& +4 "core" &zA} }# +16 "syringe1"CL& +4 "core" &vA ~# +16 "syringe1"C & +3 "core" à&ZBL ~# +17 "syringe1"CB& +3 "core" &uB # +17 "syringe1"C5& +3 "core" õ&qC\\ {# +17 "syringe1"C3& +3 "core" ó&YCꊽ }# +17 "syringe1"C8& +4 "core" ø&7}D }# +16 "syringe1"C4& +3 "core" ô&WE:늻 |# +17 "syringe1"Cc& +4 "core" &sE ~# +16 "syringe1"CN& +3 "core" &wF # +17 "syringe1"C:& +3 "core" ú&F ~# +17 "syringe1"C?& +3 "core" ÿ&{G {# +17 "syringe1"C@& +3 "core" &}G |# +17 "syringe1"C-& +4 "core" í&gH!㊻ ~# +16 "syringe1"C6& +3 "core" ö&H }# +17 "syringe1"C!& +3 "core" á&#I( }# +17 "syringe1"C& +3 "core" ß&@nI |# +17 "syringe1"C>& +3 "core" þ&b~Jn |# +17 "syringe1"CH& +3 "core" &.K芹 ~# +17 "syringe1"CI& +3 "core" &xK芵 |# +12 '/~B |< +13 "syringe1"CJ% +4 "core" %/\\Bӊ] +3 #"pump"Reached 12.8 of 15.5psi after pumping 1614uL (0.91 strokes) +2 .Air.in :slow +5 "syringe1"C>d P]z<2 +6 CA& +3 ."pump"Air.hold +6 "syringe1"C"+ +9 "core" & Mk # + â+ +4 "syringe1"C/% +4 CO& +4 "core" ï%/B +5 "syringe1"C;% +4 "core" &FMNk # + û%/B +3 "syringe1"CE& +6 ."pump"Air.in :compressLysate +2 "syringe1"C>dN I P]z<2 +13 "core" &gO # +4 ."pump"Air.seek :evac +3 "syringe1"C'| +6 CR& +5 "core" &ArP% # +3 "syringe1"C7& +4 "core" ÷& cP%⊱ # +6 "syringe1"CS& +4 "core" &RP< }# +14 "syringe1"CT& +3 "core" &iPG ~# +17 "syringe1"CV& +3 "core" &XPB +17 "syringe1"C=& +3 "core" ý&ZPF< +17 "syringe1"CW& +3 "core" &]PI] +17 "syringe1"CM& +3 "core" &8P? +17 "syringe1"CY& +4 "core" &OPC +16 "syringe1"CX& +3 "core" &PEŋ +17 "syringe1"C]& +4 "core" &QPA }# +16 "syringe1"C^& +3 "core" &'{P^, }# +17 "syringe1"CK& +3 "core" &EyPߌ( }# +12 Ã'{?^ڌT }< +4 "syringe1"CP% +4 "core" %:?hٌI < +3 #"pump"Reached 15.5 of 15.5psi after pumping 1647uL (0.93 strokes) +2 .Air.in :slow +5 "syringe1"C>d P]z<2 +7 ."pump"Air.hold +3 "syringe1"Ca+ +6 C`& +5 "core" + + &QYk0 # +4 "syringe1"C\\% +4 C9& +4 "core" %?V_ + ù&Qi# # +5 "syringe1"C[& +4 "core" &Q6 # +5 "syringe1"Cj& +3 "core" & R>> # +15 "syringe1"Cd& +3 "core" & )R # +17 "syringe1"Cg& +4 "core" &/8S # +3 "syringe1"Ch% +4 "core" %?W +3 "syringe1"Ci% +4 "core" %?] +3 ."pump"Air.in :compressLysate +2 "syringe1"C>dN I P]z<2 +9 ."pump"Air.seek :evac +3 "syringe1"C_& +5 Ck'| +8 "core" &vEU\  # +6 "syringe1"Cl& +5 "core" &\\pU= # +4 "syringe1"Ce& +3 "core" &rXV-ۋw # +14 "syringe1"Cf& +4 "core" &vWڋT # +16 "syringe1"Cm& +3 "core" & mX݋ }# +17 "syringe1"Cn& +3 "core" &RApY܊ |# +17 "syringe1"C& +3 "core" Þ&tZڊ }# +11 "Can"j\\.zs~J a +6 "syringe1"Co& +3 "core" &x[ڊ {# +5 !"preston#2"o: Syringe::LysatePlungerStuck : mesg"3at < 585uL during Lysis Syringe #1 (LyS1) move: @volumef585.18730158730159:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +8 .Lysis1.in :default +2 "syringe1"C?d P[<2 +7 ."preston#2"Lysis1.seek 555uL +4 "syringe1"Cp( +7 "core" Ô( \ u]Eˊ }# +91 ( +3 "syringe1"Cr% +4 "core" %V<׋ ed P]z<2 +8 ."pump"Air.hold +3 "syringe1"Ct+ +5 "core" + +4 "syringe1"Cv% +6 "core" %dN I P]z<2 +11 ."pump"Air.seek :evac +3 "syringe1"C'| +6 "core" ×&b`{ t +5 "syringe1"C& +4 "core" Ý&b2 x +3 "syringe1"C#& +4 "core" ã&b$ u +17 "syringe1"C+& +3 "core" ë&Mbz ~ +17 "syringe1"C,& +3 "core" ì&bu +17 "syringe1"C& +3 "core" Æ& Rbʋ ~#u +17 "syringe1"C& +3 "core" Ð&_b܋ |#| +17 "syringe1"C& +3 "core" Î&>b #~ +17 "syringe1"C& +3 "core" Ò&&`bЌ #v +17 "syringe1"C& +3 "core" Ö& ,db֋ # +17 "syringe1"Cq& +3 "core" &&7gb͋ }#x +17 "syringe1"C & +6 "core" Ì&-Ahc }#v +14 "syringe1"CU& +3 "core" &5FNc2 #| +17 "syringe1"C& +3 "core" Ø&>Oicb# #y +17 "syringe1"C$& +3 "core" ä&HSbc ~#{ +17 "syringe1"C.& +3 "core" î&SZJc #t +17 "syringe1"C0& +3 "core" ð&^evd  }#w +17 "syringe1"C~& +3 "core" &lpgdQ  ~#r +17 "syringe1"C2& +3 "core" ò&zxed3 #} +17 "syringe1"Cb& +4 "core" &Kd }#{ +16 "syringe1"CQ& +4 "core" &we* ~#| +16 "syringe1"CL& +6 "core" &Qeq # +14 "syringe1"C & +3 "core" à&We #m +17 "syringe1"CB& +3 "core" &lfM }#j +17 "syringe1"C5& +3 "core" õ&Nf\ w |#k +17 "syringe1"C3& +3 "core" ó&wg@ }#d +17 "syringe1"C8& +3 "core" ø&xg v |#X +17 "syringe1"C4& +3 "core" ô&BK{h Y }#^ +17 "syringe1"Cc& +3 "core" &opi0 |#T +17 "syringe1"CN& +3 "core" &vj\ #O +2 Ù' +4 "syringe1"C:% +4 "core" ú%|[4 }d P]z<2 +6 ."pump"Air.hold +2 "syringe1"C-+ +10 "core" í+ +3 "syringe1"C6& +4 "core" ö&%m5 ? +3 "carousel0"F!% +3 "core" ơ%S\\= \  +6 ."preston#2"Air.in :glide +2 "syringe1"C>d  P]z<2 +9 ."preston#2"Air.seek :fullVent +6 "syringe1"C'݇ +160 "core" ß' +2 ."preston#2"Handoff.select :waste +2 "valve0"D +52 #"preston#2"Recovered 800uL lysate @13.9psi +2 .delay 3 seconds +303 "syringe1"C>% +6 "core" þ%݇/ +2 #"preston#2"After 3 delay Recovered 800uL lysate @0.9psi +1386 #-> slot +3794 #-> #SC20 was processed manually as a Lyse Hold. +2145 #-> slot 30=>:microSPR_phyto +470 #-> Cart.dry +2282 "Can"m\\/zt?7 +1143 #"preston#2"-> start {fire lysis, archive} +21 #"detached from preston#2"Selecting Cartridge 30 +6 "carousel0"FH% +10 "core" %S[ \  +3 "valve0"DI% +4 "core" %B(*OQ +4 ."detached from preston#2"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +7 "valve1"EJ& +6 "core" & N<{OS # +3 ."detached from preston#2"PinPort.in :unlocking +3 "valve1"E? B2 +7 ."detached from preston#2"PinPort.seek :clear +4 "valve1"EA( +81 "core" ( +2 ."detached from preston#2"Carousel.in :default +2 "carousel0"F><` F 22 +8 F/% +4 "core" Ư%S\\} \  +3 ."detached from preston#2"Carousel.home.seek 20 +3 "carousel0"FO' +22 "core" ' +2 "carousel0"F;% +5 "core" ƻ% P]z-2 +3 "core" %{Ł n +3 ."Air"Air.seek :clear +8 ."Lysis2"Lysis2.in :unlocking +8 "syringe1"CT' +4 "syringe0"B> P\\k(2 +8 ."Lysis2"Lysis2.seek :clear +3 "syringe0"BV'j +276 "core" ' +126 ·( +47 ' +3 "syringe1"C=& +4 "core" ý&%m6 > +5 ."Lysis1"Lysis1.in :unlocking +4 "valve0"DW% +2 "syringe1"C? P[(2 +10 ."Lysis1"Lysis1.seek :clear +7 "syringe1"CM( +7 "core" %B("Oi +4 ."Plunge"Plunge.in :unlocking +5 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +3 "valve0"DY'8 +183 "core" ' +97 ( +3 ."detached from preston#2"Carousel.seek 30 +4 "carousel0"FX'X +229 "core" ' +11 "carousel0"F]% +3 "core" %X.} 2 +4 ."detached from preston#2"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +11 "syringe1"C^& +4 "core" &l[ m +4 ."detached from preston#2"Lysis1.in :fast +6 "syringe1"C?d P[<2 +10 ."detached from preston#2"Lysis1.seek :locked +4 "syringe1"CK( +9 "core" ' [O}!  +2 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 30 + 3 counts (sensed 30 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi:@pastThresholdF: @voltagef14.866994599901817: @currentiT: @type2F:@outPressi!:\ @lostF: @inPressi}:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni[: @pastRLSF: @tagi]: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ' +238 ( +3 "valve0"Du% +8 "core" %8[?O{ { +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +3 "valve0"DP'Z +8 "syringe1"C`% +7 "core" %ݩc +3 ."Air"Air.in :fast +6 "syringe1"C> P]z<2 +10 ."Air"Air.seek :locked +5 "syringe1"C\\'6 +58 "core" ' +45 ' +3 ."detached from preston#2"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +8 F9% +3 "core" ƹ%X^}N  +7 #"detached from preston#2"Powering cartridge bus +264 "cartridge_broadcast" ( +4 ."detached from preston#2"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from preston#2"PuckHeater.configure :default #ambient 28.8C +3 "Cartridge" \ :D H  +8 ."detached from preston#2"Gate.power :heat,:ON,limit to 5000mA +6 "Cartridge" [y +4 "cartridge" yGBGB +5 "Cartridge" "y  +5 "cartridge" y _S&0J59913 U&0(CJ +4 #"detached from preston#2"Mounted lysis Cartridge board#J59913 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +11 "valve0"Dj% +3 "core" %ZG0>Oz , +3 "syringe1"Cd& +8 "core" &g j +3 "syringe1"Cg% +3 "core" %6h3 +3 "carousel0"Fh% +4 "core" %X}d" \ +4 #"FILTERING"Preparing to filter microSPR_phyto cartridge in slot 30 +4 "valve0"Di% +9 "core" %ZGHAOz  +2 "syringe1"C_& +6 "core" &c; m +2 "syringe1"Cl% +4 "core" %6bU +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"Ge& +9 "core" &d~ +41 "carousel1"Gf& +3 "core" &r +47 "carousel1"Gm& +3 "core" & +48 "carousel1"Gn& +4 "core" &t} +45 "carousel1"G& +3 "core" Ǟ& +47 "carousel1"Go& +4 "core" &- +46 "carousel1"G& +3 "core" ǃ&\ +47 "carousel1"Gr& +4 "core" &k+ +47 "carousel1"Gs& +3 "core" &* +47 "carousel1"Ga& +4 "core" & +46 "carousel1"Gv& +3 "core" & +47 "carousel1"Gw& +3 "core" & +47 "carousel1"G|& +3 "core" &G +47 "carousel1"G}& +3 "core" & +5 "Can"m\\/zt'oa + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 29C (drifted 6%) +3 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +10 .PhaseSeparator.in :filling + "carousel1"G?")") +8 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +6 "core" , +4 ."FILTERING"Gate.power :sampler,:ON +666 "core" ,a +7 #"FILTERING"Head pressure reached 3.82psi in 7 seconds +7 "carousel0"F% +12 ."sealing"Air.in :slow +4 "core" Ƃ%X}Cc \ +2 "syringe1"C>d P]z<2 +12 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +5 ."sealing"Air.seek :sealed +6 ."FILTERING"SamplePump.setPosition! 0ml +6 "syringe1"C' +2 "sampler0"H7 +9 ."FILTERING"Bypass.open +3 "sampler1"I1 +154 "core" Ʌ1 +73 "ambient0"JZ% +3 "core" % t +3 ."FILTERING"Intake.open +3 "sampler0"H1 +145 "core" Ȕ1 +2 "ambient0"J% +6 "core" ʇ% p +4 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +3 "sampler0"Hz% +7 "core" %w v +42 "sampler0"HG% +4 "core" % v +46 "sampler0"H{% +4 "core" %g x +46 "sampler0"H % +6 "core" ȉ%zh v +55 "sampler0"H1% +4 "core" ȱ%g w +35 "sampler0"Hy% +4 "core" %V w +4 "Can"m\\/zsf +2 #"FILTERING"Vacuum tare is 54% of full scale at 29C (drifted 2%) +3 "ambient0"J\ % +8 "core" ʊ% s +2 "ambient0"Jk% +4 "core" % s +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +9 ."FILTERING"Abs.hold +2 "ambient0"J+ +6 "core" ʏ+ +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +7 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +13 "core" Ǒ, +3 "sampler0"H% +4 "core" ȓ% w +3 "sampler0"H% +5 "core" ȕ%p u +2 #"FILTERING"Waiting for sampler to finish priming... +3 .delayUntil "23:00:45.78" +462 "core" Ä' +4061 "Can"m\\/zsAT +13 ."FILTERING"SamplePump.in :start +3 "sampler0"H>^ }n#(@13 22Q +10 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"Hp'6 +53 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }n#(@13 22Q +1290 "core" ' +2 "sampler0"H% +5 "core" ț%6ω 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +7 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +8 ."FILTERING"Exhaust.open +2 "sampler0"H2 +149 "core" Ț2 +74 ."closeBypass"Bypass.close +3 "sampler1"I/ +155 "core" ɗ/ +73 "sampler0"H% +4 "core" ȝ%ъ( +3 "ambient0"J#% +6 "Can"l\\.zsB%E +2 "core" ʣ% +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +9 ."FILTERING"Abs.hold +3 "ambient0"J&+ +6 "core" ʦ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G', +10 "core" ǧ, +2 "ambient0"J% +4 "core" ʁ% +3 "sampler0"H(% +6 "core" Ȩ%Ɋ: +2 "sampler0"H)% +4 "core" ȩ%Ί8 +6 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }n#(@L13 22Q +10 ."FILTERING"SamplePump.seek 200ml,13:50 +4 "sampler0"H+'G +53 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }n#(@L13 22Q +7700 #"preston#2"-> exit +5 #Session Ended +4118 "Can"i\\/zsHY! +11961 j\\/ z\ sJY,5 +2410 "core" ȫ' +2 "sampler0"H,% +4 "core" Ȭ%G 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +9 ."FILTERING"Bypass.open +2 "sampler1"I1 +153 "core" Ɇ1 +3 ."FILTERING"Intake.close +2 "sampler0"Ht/ +142 "core" / +2 "carousel0"F% +4 "core" Ɛ%X}g \ +6 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :crack +3 "valve0"D( +169 "core" Ď( +78 ."FILTERING"Toroid.seek :cartridge +3 "valve0"D(m +207 "core" Ē( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +2 "valve0"D( +373 "core" Ė( +2 "ambient0"Jx% +4 "core" % +5 "Can"j\\/ z\ shL2/5 + "ambient0"Jq% +4 "core" % +3 "ambient0"J % +3 "core" ʌ% +4 #"FILTERING"Pressurizing cartridge to 14psi above 14.0psia +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +10 ."FILTERING"Intake.open +2 "sampler0"H 1 +143 "core" ȍ1 +2 ."FILTERING"Bypass.close +2 "sampler1"IU/ +149 "core" / +2 ."FILTERING"Exhaust.close +4 "sampler0"H0 +148 "core" Ș0 +3 "sampler0"H$% +4 "core" Ȥ%9 +3 "ambient0"J.% +4 "core" ʮ% +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J0+ +5 "core" ʰ+ +4 ."FILTERING"PhaseSeparator.hold + "carousel1"G~, +8 "core" , +3 "sampler0"H2% +5 "core" Ȳ%9 +3 "sampler0"Hb% +4 "core" %9 +4 ."FILTERING"SamplePump.in :charge + "sampler0"H>nu0Y3 22Q +8 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +5 "sampler0"HQ' +1375 "core" 'db 2 +5 "sampler0"HL% +4 "core" %di 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +8 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +71 ."FILTERING"Intake.close +2 "sampler0"H / +148 "core" Ƞ/ +2 "ambient0"JB% +6 "core" %& +2 #"FILTERING"Pressurized cartridge with 0.801ml to 14.9psi +2 #Isolated @27.0psia (13.0psi) +5 #Verifying cartridge holds at least 0.65..0.85 of 27.0psia +5 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"J5% +6 "core" ʵ%, +44 "ambient0"J3% +4 "core" ʳ%' +46 "ambient0"J % +4 "core" ʋ%/ +46 "ambient0"J8% +4 "core" ʸ%, +46 "ambient0"J4% +4 "core" ʴ%-h +46 "ambient0"Jc% +5 "core" %* +45 "ambient0"JN% +4 "core" %, +46 "ambient0"J:% +3 "core" ʺ%' +47 "ambient0"J?% +3 "core" ʿ% +47 "ambient0"J@% +4 "core" %&U +3 #"FILTERING"Cartridge pressure fell 4% to 25.9psia +2 "carousel0"F6% +8 "core" ƶ%X}chi \ +3 #"FILTERING"Sampling 100.0ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +7 ."FILTERING"Exhaust.open +2 "sampler0"H!2 +150 "core" ȡ2 +2 ."FILTERING"Intake.open +3 "sampler0"H1 +142 "core" ș1 +5 "Can"j\\/ z\ sfMy1|_ +2 "ambient0"J-% +3 "core" ʭ%C +6 "ambient0"J>% +4 "core" ʾ%E +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +6 ."FILTERING"Abs.hold +2 "ambient0"JH+ +10 "core" + +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GI, +9 "core" , +2 "ambient0"JJ% +5 "core" %+ +3 "sampler0"H/% +4 "core" ȯ%Ɗ  +3 "sampler0"H;% +4 "core" Ȼ%7  +8 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ n#(Y@L@3 22Q +7 ."FILTERING"SamplePump.seek 100ml,6:04 +3 "sampler0"HE'# +56 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d n#(Y@L@3 22Q +227 HR% +3 "core" %\ H T ~2 +3 "ambient0"JS% +4 "core" %  z +2 #"sampling"Cartridge 30 filtering 2/100ml at 0.69ml/s with 14.4psi vacuum +4954 "Can"l\\/zsOY6& +1040 "sampler0"HF% +3 "core" % pR ~2 +3 "ambient0"J*% +4 "core" ʪ%$ | +2 #"sampling"Cartridge 30 filtering 44/100ml at 0.71ml/s with 8.9psi vacuum +5987 "sampler0"HD% +3 "core" % I. 2 +3 "ambient0"J% +5 "core" ʟ%0 { +2 #"sampling"Cartridge 30 filtering 82/100ml at 0.64ml/s with 11.8psi vacuum +2942 "core" ' +2 "sampler0"HC% +6 "core" %#+& 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +3 "carousel1"G6 +9 ."closeIntake"Intake.close +3 "sampler0"H=/ +5 ."closeExhaust"Exhaust.close +6 "sampler0"HW0 +7 ."closeHandoff"Handoff.select :close +4 "carousel0"F% +7 "valve0"D0 +7 "core" Ɯ%X}fa \ +7 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :bypass +4 "valve0"D<( +91 "core" Ƚ/ +22 0 +242 ļ( +6 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +3 "valve0"D]( +354 "core" ( +3 #"FILTERING"Sampled 100.0ml +4 "carousel0"F^% +6 "core" %X}e \ +10 "valve0"DO% +4 "core" %ZG0OV , +3 "syringe1"Cu& +4 "core" &Mf g +3 "syringe1"C`% +4 "core" %LX +3 "carousel0"FM% +4 "core" %X}f \ +6 !"detached from preston"o:Cmd::Error: mesg"4Cannot load cartridge while in PROCESSING state:@timestampu: Time #q>#:bt[";/home/esp/esp2/utils/auv/cmd.rb:258:in `loadCartridge'":/home/esp/esp2/utils/auv/cmd.rb:148:in `runCartridge'"6/home/esp/esp2/utils/auv/cmd.rb:214:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:201:in `each'"6/home/esp/esp2/utils/auv/cmd.rb:201:in `runCarts'"2/home/esp/esp2/utils/auv/cmd.rb:221:in `fire'"1/home/esp/esp2/utils/auv/cmd.rb:62:in `fire'"#preston:1:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 "carousel0"F9% +3 #"detached from preston"Waiting to Resume from Checkpoint "Selecting Cartridge 20" +8 "core" ƹ%X}e \ +11 ."PROCESSING"SPR.command "stats_on" +73 "carousel0"FY% +3 "core" %X}wg \ +7 ."PROCESSING"SPR.command "start 0 1" +69 ."SPRlogger"SPR.start /var/log/esp/spr26jul3123h.spr.bz2 +89 ."sourcePumpShutdown"Gate.power :sampler,:OFF +169 ."PROCESSING"Handoff.select :waste +2 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"CT% +6 "core" %a +44 "syringe1"C[% +3 "core" %h +47 "syringe1"C"% +3 "core" â%{ +91 "syringe1"Cj% +4 "core" % +5 "syringe1"Cd% +4 "core" % +43 "syringe1"Cg% +3 "core" % +47 "syringe1"Ch% +3 "core" %q +47 "syringe1"Ci% +4 "core" %t +47 "syringe1"C_% +4 "core" %s +46 "syringe1"Cl% +3 "core" %b +47 "syringe1"Ce% +3 "core" %h +47 "syringe1"Cf% +3 "core" %v +49 "syringe1"Cm% +6 "core" % +2 "Can"o\\/zt9? +40 "syringe1"Cn% +3 "core" % +5 "Can"o\\/zt)a? + #"PROCESSING"Syringe tare is 51% of full scale at 29C (drifted -0%) +5 .PinPort.in :locking +4 "valve1"E? B22 +5 ."PROCESSING"PinPort.seek :locked +3 "valve1"E( N +80 "core" Ş( +4 ."posArchive"Archive.in :fast +5 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +8 ."PROCESSING"Lysis1.seek 1250uL +6 "syringe0"B>d P\\k<2 +5 ."posArchive"Archive.seek :empty +9 "syringe1"Co( +8 ."posLyS2"Lysis2.seek 950uL +6 "syringe0"B( +4 Br' +242 "core" ( +260 ' +329 ƒ( +2 "carousel0"Fs% +5 "core" %X}v \ +5 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +6 .PinPort.seek :locked +3 "valve1"Ea( N +7 "core" ( +2 ."PROCESSING"Handoff.select :waste +5 .Plunge.seek :puckBottom +3 "valve0"Dv'B +128 "core" ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"Cw% +7 "core" % +44 "syringe1"C|% +4 "core" % +46 "syringe1"C}% +3 "core" % +47 "syringe1"C% +4 "core" Â% +46 "syringe1"C% +3 "core" Å% +47 "syringe1"CZ% +3 "core" % +47 "syringe1"C\\% +3 "core" % +47 "syringe1"C% +4 "core" Ô% +47 "syringe1"C% +3 "core" Ç% +47 "syringe1"Cz% +4 "core" % +46 "syringe1"CG% +4 "core" % +46 "syringe1"C{% +4 "core" % +46 "syringe1"C % +3 "core" É% +47 "syringe1"C1% +3 "core" ñ% +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi + #Air stroke #1 of 1 +3 "syringe1"Cy% +8 "core" % +6 ."PROCESSING"Air.in :pressurize +3 "syringe1"C>@N Z P]z<2 +7 ."PROCESSING"Air.seek :pressurized +2 "syringe1"C\ ' +518 "core" Ê' +2 "syringe1"Ck% +4 "core" %"S < +3 #"PROCESSING"0.3psi after pressurize +3 .Air.in :compress +3 "syringe1"C>N Z P]zP2 +7 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +654 "core" Ó' +3 "syringe1"C% +4 "core" Õ%|4K jPj +2 #"PROCESSING"2.5psi after compress +3 "syringe1"C% +11 "core" %|4 ov +40 "syringe1"C%% +3 "core" å%|4p w +56 "syringe1"C% +5 "core" Û%|4o m +35 "syringe1"C% +4 "core" Ú%|4j | +47 "syringe1"C% +4 "core" Ï%|4kQ j +45 "syringe1"C% +4 "core" Ñ%|4sb h +46 "syringe1"C% +3 "core" ×%|4n j +47 "syringe1"C% +4 "core" Ý%|4o +46 "syringe1"C#% +3 "core" ã%|4l f +47 "syringe1"C% +3 "core" Á%|4k m +47 "syringe1"C(% +4 "core" è%|4k p +46 "syringe1"C)% +3 "core" é%|4k j +47 "syringe1"Cp% +3 "core" %|4h l +47 "syringe1"C,% +3 "core" ì%|4h p +47 "syringe1"C% +3 "core" Æ%|4j e +47 "syringe1"C&% +4 "core" æ%|4j f +47 "syringe1"C'% +4 "core" ç%|4g i +46 "syringe1"Ct% +3 "core" %|4j s +47 "syringe1"C% +3 "core" Ð%|4b n +48 "syringe1"C% +7 "core" È%|4c t +42 "syringe1"C% +4 "core" Î%|4d j +47 "syringe1"C% +4 "core" Ò%|4h h +45 "syringe1"Cx% +3 "core" %|4^ r +47 "syringe1"Cq% +3 "core" %|4] v +47 "syringe1"C % +4 "core" Ì%|4` ` +46 "syringe1"C % +3 "core" Í%|4e z +3 #"PROCESSING"0.2psi after compress (stable) +2 .Air.in :slow +4 "syringe1"C>d P]z<2 +7 ."PROCESSING"Air.hold +2 "syringe1"CU+ +6 "core" + +3 "syringe1"C% +5 "core" Ø%|4ky \\ +2 "syringe1"C$% +4 "core" ä%|4s n +2 #"PROCESSING"at 0.1psi Opened to waste +2 .Handoff.select :blocked +3 "valve0"D0 +50 ."PROCESSING"Plunge.seek :puckTop +4 "valve0"D.': +84 "core" Į' +3 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi +2 #Air stroke #1 of 8 +2 "syringe1"C2% +11 "core" ò%|4]v d +3 ."PROCESSING"Air.in :compress + "syringe1"C>N  P]zP2 +7 ."PROCESSING"Air.seek :evac +3 "syringe1"Cb'| +6 "core" ' +3 "syringe1"C+% +5 "core" ë%|4so +3 #"PROCESSING"1.3psi after compress +2 "syringe1"CL% +8 "core" %|4 u +43 "syringe1"C % +4 "core" à%|4]w p +46 "syringe1"CB% +3 "core" %|4\\u s +47 "syringe1"C5% +3 "core" õ%|4[| i +47 "syringe1"C0% +3 "core" ð%|4\\ h +47 "syringe1"C3% +3 "core" ó%|4] p +47 "syringe1"C~% +3 "core" %|4` n +47 "syringe1"C % +4 "core" Ë%|4_ q +46 "syringe1"C8% +3 "core" ø%|4^ p +47 "syringe1"C4% +4 "core" ô%|4^ r +46 "syringe1"Cc% +4 "core" %|4Y e +46 "syringe1"CN% +4 "core" %|4Y u +47 "syringe1"C:% +5 "core" ú%|4V s +44 "syringe1"C?% +3 "core" ÿ%|4Y c +47 "syringe1"C@% +3 "core" %|4X t +47 "syringe1"C6% +3 "core" ö%|4V i +48 "syringe1"C!% +3 "core" á%|4x y +47 "syringe1"C% +3 "core" Ù%|4X h +77 "syringe1"C-% +5 "core" í%|4P a +15 "syringe1"C>% +4 "core" þ%|4W x +46 "syringe1"CJ% +3 "core" %|4T ^ +47 "syringe1"C/% +4 "core" ï%|4R l +3 #"PROCESSING"1.7psi after compress (stable) +3 .Air.in :gulp +14 "syringe1"C>N  P]z<2 +7 ."PROCESSING"Air.seek :topVent +9 "syringe1"C;'6 +639 "core" û' +5 "syringe1"CR% +8 "core" %6BɪƂ < +2 #"PROCESSING"1.6psi after gulp +3 "syringe1"CS% +9 "core" %6ɪ +41 "syringe1"CQ% +3 "core" %6ɟ +47 "syringe1"CF% +3 "core" %6ɭ +47 "syringe1"C*% +4 "core" ê%6ɨ +46 "syringe1"CD% +3 "core" %6ɬ +47 "syringe1"C% +3 "core" ß%6ɰ +48 "syringe1"CC% +3 "core" %6ɦ +47 "syringe1"C% +3 "core" Ü%6ɛ +47 "syringe1"C=% +4 "core" ý%6ɢ +46 "syringe1"CW% +3 "core" %6ɝ +47 "syringe1"CH% +3 "core" %6ɬ +47 "syringe1"CI% +7 "core" %6ɭ +4 #"PROCESSING"1.7psi after gulp (stable) +3 #Air stroke #2 of 8 +2 "syringe1"C% +9 "core" Ö%6ɢ +4 ."PROCESSING"Air.in :pressurize +4 "syringe1"C>@N  P]z<2 +8 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C<' +630 "core" ü' +2 "syringe1"C^% +4 "core" %=S < +2 #"PROCESSING"2.2psi after pressurize +3 .Air.in :compress +4 "syringe1"C>N  P]zP2 +7 ."PROCESSING"Air.seek :evac +3 "syringe1"CO'| +654 "core" ' +2 "syringe1"Cu% +4 "core" %|4?6 ePr +2 #"PROCESSING"13.5psi after compress +3 "syringe1"C`% +9 "core" %|49 n +41 "syringe1"CM% +4 "core" %|4} s +46 "syringe1"C9% +3 "core" ù%|4 t +48 "syringe1"CY% +6 "core" %|45 l +43 "syringe1"CT% +3 "core" %|4M p +48 "syringe1"C[% +3 "core" %|4}` e +46 "syringe1"C"% +3 "core" â%|4h n +47 "syringe1"Cj% +3 "core" %|4}s u +47 "syringe1"Cd% +4 "core" %|4v _ +46 "syringe1"Cg% +3 "core" %|4t c +47 "syringe1"Ch% +5 "core" %|4s h +45 "syringe1"Ci% +3 "core" %|4y l +47 "syringe1"C_% +3 "core" %|4{ l +47 "syringe1"Cl% +3 "core" %|4z o +47 "syringe1"Ce% +3 "core" %|4z r +47 "syringe1"Cf% +3 "core" %|4y o +48 "syringe1"Cm% +3 "core" %|4o c +47 "syringe1"Cn% +3 "core" %|4w q +47 "syringe1"CA% +4 "core" %|4u e +51 "syringe1"CK% +4 "core" %|4o t +42 "syringe1"C7% +7 "core" ÷%|4t a +42 "syringe1"CV% +3 "core" %|4p c +48 "syringe1"Cs% +3 "core" %|4C t +46 "syringe1"Cw% +3 "core" %|4s _ +3 #"PROCESSING"15.8psi after compress (stable) +3 .Air.in :gulp +4 "syringe1"C>N  P]z<2 +6 ."PROCESSING"Air.seek :topVent +3 "syringe1"C|'6 +637 "core" ' +2 "syringe1"C}% +4 "core" %6Qɤa < +3 #"PROCESSING"15.1psi after gulp +2 "syringe1"C% +8 "core" Þ%6ɦ, +42 "syringe1"C% +4 "core" Â%6ɢc +46 "syringe1"CP% +4 "core" %6ɢ` +46 "syringe1"C% +4 "core" Å%6ɦd +46 "syringe1"CZ% +4 "core" %6ɜc +46 "syringe1"C\\% +4 "core" %6ɦc +47 "syringe1"C% +4 "core" Ô%6ɫ +46 "syringe1"C% +4 "core" Ç%6ɡ`~ +46 "syringe1"Cz% +4 "core" %6ɠcn +46 "syringe1"CG% +3 "core" %6ɪcX +47 "syringe1"C{% +5 "core" %6ɨ^Y +45 "syringe1"C % +3 "core" É%6ɜ[[ +49 "syringe1"C1% +6 "core" ñ%6ɧ\\\\ +42 "syringe1"Cy% +3 "core" %6ɫYc +47 "syringe1"C% +3 "core" Ä%6ɩXv +47 "syringe1"Ck% +4 "core" %6ɣ`_ +46 "syringe1"C\ % +3 "core" Ê%6ɥ[X +47 "syringe1"C% +4 "core" Õ%6ɦ\\Z +2 #"PROCESSING"15.1psi after gulp (stable) +2 #Air stroke #3 of 8 +4 "syringe1"C% +8 "core" %6ɪ]J +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +6 ."PROCESSING"Air.seek :pressurized +6 "syringe1"C%' +631 "core" å' +2 "syringe1"C% +4 "core" Û%GS < +2 #"PROCESSING"14.8psi after pressurize +4 .Air.in :compress +4 "syringe1"C>N  P]zP2 +6 ."PROCESSING"Air.seek :evac +2 "syringe1"C'| +340 "Can"j\\/zs@\\E3 +114 "core" Ú'kp>, gP +4 "syringe1"C% +5 "core" Ï%k>. kP +2 #"PROCESSING"Reached 20.2 of 20.0psi after pumping 5211uL (2.93 strokes) +2 .Air.in :slow +3 "syringe1"C>d P]z<2 +10 ."PROCESSING"Air.hold +6 "syringe1"C+ +6 "core" Ñ+ +3 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"D'B +86 "core" ė' +2 ."PROCESSING"delay 3 seconds +302 .Handoff.select :waste +2 "valve0"D +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C% +7 "core" Ý%k>/ +44 "syringe1"C#% +4 "core" ã%k>7 +48 "syringe1"C% +5 "core" Á%k>9n +43 "syringe1"C(% +4 "core" è%k>6 +46 "syringe1"C)% +3 "core" é%k>< +47 "syringe1"Cp% +3 "core" %k>:[ +48 "syringe1"C,% +6 "core" ì%k>@ +43 "syringe1"C% +3 "core" Æ%k>4 +48 "syringe1"C&% +3 "core" æ%k>9 +47 "syringe1"C'% +3 "core" ç%k>< +47 "syringe1"Ct% +3 "core" %k>>j +47 "syringe1"C% +4 "core" Ð%k>: +46 "syringe1"C% +4 "core" È%k>L +46 "syringe1"C% +3 "core" Î%k>E +47 "syringe1"C% +3 "core" Ò%k>H +47 "syringe1"Cx% +3 "core" %k>@v +47 "syringe1"Cq% +3 "core" %k>L^ +47 "syringe1"C % +3 "core" Ì%k>JV +47 "syringe1"C % +3 "core" Í%k>`L r +48 "syringe1"C% +11 "core" Ø%k>Jd +38 "syringe1"C$% +3 "core" ä%k>Fh +47 "syringe1"C2% +4 "core" ò%k>J +46 "syringe1"C+% +3 "core" ë%k>= +47 "syringe1"CL% +3 "core" %k>J +47 "syringe1"C % +3 "core" à%k>= +47 "syringe1"C% +3 "core" Ó%k><} +47 "syringe1"CB% +3 "core" %k>Cm +47 "syringe1"C5% +3 "core" õ%k>Oo +47 "syringe1"Cv% +3 "core" %k>Jo +47 "syringe1"C0% +3 "core" ð%k>Zo x +47 "syringe1"C3% +4 "core" ó%k>Ij +46 "syringe1"CU% +4 "core" %k>Q +6 ."PROCESSING"delay 1:00 +6003 .Handoff.select :blocked +2 "valve0"D0 +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi +2 "syringe1"C~% +6 "core" %k>L} +51 "syringe1"C % +4 "core" Ë%k>Nz +40 "syringe1"C8% +3 "core" ø%k>Q~ +47 "syringe1"C4% +4 "core" ô%k>S|  +46 "syringe1"Cc% +3 "core" %k>Q| +47 "syringe1"CN% +3 "core" %k>Mxu +47 "syringe1"C:% +3 "core" ú%k>Kys +47 "syringe1"C?% +3 "core" ÿ%k>Qu +47 "syringe1"C@% +3 "core" %k>Iw +47 "syringe1"C6% +4 "core" ö%k>Mz +46 "syringe1"C!% +3 "core" á%k>Xr z +47 "syringe1"C% +3 "core" Ù%k>Ly +47 "syringe1"C-% +3 "core" í%k>Ry +47 "syringe1"C>% +3 "core" þ%k>Kuw +47 "syringe1"CJ% +3 "core" %k>Vvq | +47 "syringe1"C/% +3 "core" ï%k>Hso +47 "syringe1"Cb% +6 "core" %k>Fp +44 "syringe1"CR% +3 "core" %k>Mv +48 "syringe1"CS% +4 "core" %k>Ir +45 "syringe1"CQ% +4 "core" %k>Wr { +46 "syringe1"CF% +3 "core" %k>Vn | +47 "syringe1"C*% +3 "core" ê%k>Rn +47 "syringe1"CD% +3 "core" %k>Lp +47 "syringe1"C% +3 "core" ß%k>Mi~ +47 "syringe1"CC% +3 "core" %k>No +47 "syringe1"C% +3 "core" Ü%k>Oh +47 "syringe1"C=% +3 "core" ý%k>Mm +47 "syringe1"CW% +3 "core" %k>Cn +47 "syringe1"CH% +3 "core" %k>@ +47 "syringe1"CI% +4 "core" %k>Qm +46 "syringe1"C% +4 "core" Ö%k>Tj ~ +46 "syringe1"C;% +3 "core" û%k>Ohz +47 "syringe1"C^% +3 "core" %k>Nc{ +47 "syringe1"C<% +5 "core" ü%k>Lg +45 "syringe1"Cu% +3 "core" %k>Mf +48 "syringe1"C`% +10 "core" %k>Gj +39 "syringe1"CM% +3 "core" %k>Hh +47 "syringe1"C9% +3 "core" ù%k>Ti ~ +47 "syringe1"CY% +3 "core" %k>Nh +47 "syringe1"CT% +4 "core" %k>P +47 "syringe1"C[% +4 "core" %k>Ocx +46 "syringe1"C"% +5 "core" â%k>Tb ~ +45 "syringe1"Cj% +3 "core" %k>L] +47 "syringe1"Cd% +3 "core" %k>S`  +47 "syringe1"Cg% +3 "core" %k>Qc +47 "syringe1"Ch% +3 "core" %k>K_ +3 ."PROCESSING"Handoff.select :waste +3 "valve0"D +48 #"PROCESSING"0.2psi at evacEnd +3 .Plunge.seek :pTop +6 "valve0"Di': +85 "core" ' +2 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +2 "syringe1"C>d  P]z<2 +7 ."PROCESSING"Air.seek :fullVent +2 "syringe1"C_'݇ +279 "core" ' +3 ."PROCESSING"delay 2 seconds +203 .Plunge.seek :pBottom +2 "valve0"Dl'B +86 "core" ' +2 #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +3 .Plunge.seek :locked +4 "valve0"De'Z +129 "core" ' +2 ."PROCESSING"Handoff.select :waste +3 "syringe1"Cf& +11 "core" &^j +3 ."PROCESSING"Lysis1.in :slow +2 "syringe1"C?d P[<2 +7 ."PROCESSING"Lysis1.seek 950uL +3 "syringe1"Cm( +80 "Can"k\\3/ z s=*I;Q +1326 "core" ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select :blocked +5 "valve0"D0 +47 ."PROCESSING"Plunge.seek :puckTop +7 "valve0"Dn': +87 "core" ' +4 "syringe1"CA& +9 "core" &zz]  +3 ."PROCESSING"Lysis1.seek 350uL +5 "syringe1"CK( +2801 "core" ( +2 ."PROCESSING"delay 3 seconds +304 "syringe1"C7% +4 "core" ÷%݇/Zl +4 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 +2 "syringe1"C> wk+ P]z<2 +9 CV% +4 "core" %݇/#L +3 "syringe1"Cs& +5 "core" &G_ +52 ."Airmixer"Air.seek :evac +5 "syringe1"Cw'| +11 ."PROCESSING"Lysis1.seek 190uL +8 "syringe1"CO( +751 "core" ' +5 ( +3 ."PROCESSING"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."PROCESSING"Air.seek :full +3 "syringe1"C}'6 +648 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"C( +895 "core" Þ( +3 "valve0"D% +3 "core" Ă%:7OA 2 +3 "syringe1"CP% +4 "core" %6Nɬ +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @10.4psi +3 .Plunge.seek :puckBottom +4 "valve0"D'B +85 "core" ą' +2 "valve0"DZ% +4 "core" %B(X - +3 "syringe1"C\\% +5 "core" %6ɳ +3 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @8.8psi +4 #Polling Can every 2:00 +56 "Can"l\\]/ ztBMe" +2 ."PROCESSING"PuckHeater.configure :default #ambient 28.9C +3 "Cartridge" \ :D H  +6 ."PROCESSING"PuckHeater.seek 81.3C +6 "Cartridge" 9 +2   +4 "cartridge" 9&K +109 "Cartridge" z9 +5 "cartridge" 9&S d +104 "Cartridge" G9 +3 "cartridge" 9'* d +103 "Cartridge" {9 +5 "cartridge" 9*| d +103 "Cartridge" 9 +3 "cartridge" 9. d +104 "Cartridge" 19 +3 "cartridge" 92 d +104 "Cartridge" y9 +3 "cartridge" 96~ d +104 "Cartridge" 9 +3 "cartridge" 99 d +106 "Cartridge" k9 +6 "cartridge" 9= d +104 "Cartridge" \ 9 +3 "cartridge" 9? #d +104 "Cartridge" 9 +3 "cartridge" 9B$ 'd +103 "Cartridge" 9 +3 "cartridge" 9DE8+d +104 "Cartridge" |9 +4 "cartridge" 9F80d +103 "Cartridge" 9 +3 "cartridge" 9H` 4d +104 "Cartridge" %9 +4 "cartridge" 9JP88d +103 "Cartridge" 9 +3 "cartridge" 9K8=d +106 "Cartridge" 9 +4 "cartridge" 9M8Ad +103 "Cartridge" .9 +4 "cartridge" 9Oh8Ed +103 "Cartridge" 9 +4 "cartridge" 9PeJd +104 "Cartridge" #9 +4 "cartridge" 9ReeNd +103 "Cartridge" 9 +4 "cartridge" 9SeRd +104 "Cartridge" (9 +5 "cartridge" 9UEeWd +103 "Cartridge" )9 +4 "cartridge" 9Ve[d +104 "Cartridge" p9 +4 "cartridge" 9X _d +103 "Cartridge" ,9 +4 "cartridge" 9Y_cd +121 "Cartridge" 9 +4 "cartridge" 9Zhd +113 "Cartridge" &9 +4 "cartridge" 9\\Nmd +103 "Cartridge" '9 +4 "cartridge" 9]sqd +105 "Cartridge" t9 +6 "cartridge" 9^vd +103 "Cartridge" 9 +4 "cartridge" 9`zd +105 "Cartridge" 9 +6 "cartridge" 9aKd +103 "Cartridge" 9 +4 "cartridge" 9bsd +103 "Cartridge" 9 +4 "cartridge" 9cd +103 "Cartridge" x9 +4 "cartridge" 9dd +103 "Cartridge" q9 +4 "cartridge" 9ec +106 "Cartridge" 9 +3 "cartridge" 9fd +103 "Cartridge" 9 +4 "cartridge" 9gd +103 "Cartridge" 9 +4 "cartridge" 9iDd +103 "Cartridge" $9 +5 "cartridge" 9jDd +99 "Can"i\\/\ zseQQo +4 "Cartridge" 29 +4 "cartridge" 9k*Dc +103 "Cartridge" +9 +4 "cartridge" 9l'qc +103 "Cartridge" L9 +4 "cartridge" 9m*qd +103 "Cartridge" 9 +4 "cartridge" 9nqd +108 "Cartridge" 9 +5 "cartridge" 9o,qd +103 "Cartridge" B9 +3 "cartridge" 9pc +104 "Cartridge" 59 +3 "cartridge" 9q6c +104 "Cartridge" v9 +4 "cartridge" 9rd +103 "Cartridge" 09 +4 "cartridge" 9sc +103 "Cartridge" 39 +4 "cartridge" 9t"d +103 "Cartridge" U9 +4 "cartridge" 9td +106 "Cartridge" ~9 +3 "cartridge" 9ud +104 "Cartridge" 9 +3 "cartridge" 9vd +104 "Cartridge" 89 +3 "cartridge" 9w$d +104 "Cartridge" 49 +4 "cartridge" 9xc +104 "Cartridge" c9 +4 "cartridge" 9y$d +103 "Cartridge" N9 +4 "cartridge" 9zz$c +103 "Cartridge" :9 +8 "cartridge" 9{U$d +106 "Cartridge" ?9 +3 "cartridge" 9|UPd +104 "Cartridge" @9 +3 "cartridge" 9}5Pd +104 "Cartridge" 69 +3 "cartridge" 9}Pc +103 "Cartridge" !9 +4 "cartridge" 9~Pc +103 "Cartridge" 9 +4 "cartridge" 9Pd +103 "Cartridge" -9 +4 "cartridge" 9sP d +103 "Cartridge" >9 +5 "cartridge" 9v} c +104 "Cartridge" J9 +3 "cartridge" 9APb +104 "Cartridge" /9 +3 "cartridge" 9}` +124 "Cartridge" b9 +4 "cartridge" 9}_ +103 "Cartridge" R9 +4 "cartridge" 9w}] +4 ."PROCESSING"PuckHeater.hold 2:00,81.3C +13 "Cartridge" S oV +5 "cartridge"  +8898 "Can"l\\/ z s.OXw +5 l\\/ z s.Xw +3073 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +53 "Can"k\\X/ ztDY +2 ."PROCESSING"PuckHeater.seek 80.2C +4 "Cartridge" Q   +5 F9 +6 "cartridge" 90 +7 ."PROCESSING"PuckHeater.hold 2:00,80.2C +61 "Cartridge" *   +6 "cartridge"  +8735 "Can"j\\/ zt(]l +4 j\\/ zs(]m +3245 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +53 "Can"k\\/ztD^ +3 ."PROCESSING"PuckHeater.configure :default #ambient 28.8C +2 "Cartridge" \ :D H  +11 ."PROCESSING"PuckHeater.hold 8:00,79.2C +7 "Cartridge" D 7Ç +8631 "Can"k\\/zt&b +4 k\\/zt '=b +11953 l\\r/zt&f +3 l\\r/zt &f +11954 l\\/zt&.k  +3 l\\/zt\ &ek  +11956 k] .zt&6o# +3 k] .zt &{o# +3415 "cartridge" +3 "syringe1"C% +4 "core" ß%6ɪT +2 #"PROCESSING"Lysate cooked @7.8psi +3 .Plunge.seek :puckTop +5 "valve0"DC': +84 "core" ' +3 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi +2 #Air stroke #1 of 5 +3 "syringe1"C% +9 "core" Ü%6ɥIX +2 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N G P]z<2 +6 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C=' +631 "core" ý' +2 "syringe1"CW% +3 "core" %S/ < +3 #"PROCESSING"7.9psi after pressurize +3 .Air.in :compress +3 "syringe1"C>N G P]zP2 +7 ."PROCESSING"Air.seek :evac +3 "syringe1"CH'| +554 "core" 'pp9TU iP +4 "syringe1"CI% +6 "core" %p9M kP + #"PROCESSING"Reached 41.0 of 40.0psi after pumping 1718uL (0.97 strokes) +3 .Air.in :slow +2 "syringe1"C>d P]z<2 +8 ."PROCESSING"Air.hold +2 "syringe1"C+ +5 "core" Ö+ +3 ."PROCESSING"delay 2 seconds +203 .Air.in :fast +4 "syringe1"C> P]z<2 +10 ."PROCESSING"Air.seek :full +3 "syringe1"C;'6 +628 "core" û' +2 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"D^'B +86 "core" ' +2 "syringe1"C<& +5 "core" ü&)iH +3 ."PROCESSING"Lysis1.seek 138uL +3 "syringe1"Cu(# +653 "core" ( +3 ."PROCESSING"delay 2 seconds +202 .Plunge.seek :puckTop +48 "valve0"D`': +86 "core" ' +2 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"CM( +655 "core" ( +2 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"D9'B +86 "core" Ĺ' +2 "carousel0"FY% +5 "core" %X} \ +6 "valve0"DT% +4 "core" %B(.X +5 "syringe1"C[% +6 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d 'FJ P[#2 +6 "core" %6ɣS +4 ."PROCESSING"Lysis1.seek 675uL,nil +4 "syringe1"C"( +6 Cj& +5 "core" &4)Y # +3 "syringe1"Cd& +4 "core" & ) # +8 "syringe1"Cg& +3 "core" &!*8` # +15 "syringe1"Ch& +3 "core" &:"+; # +4 "syringe1"C% +5 "core" ×%6 ɧ +8 "syringe1"C& +4 "core" Ñ&ݝ -I # +16 "syringe1"Cf& +3 "core" & !/r #r +17 "syringe1"Ci& +4 "core" &` #1 #h +4 "syringe1"Cl% +8 "core" %6ɲ +5 "syringe1"Co& +4 "core" & 3" #\\ +15 "syringe1"CA& +3 "core" &#  5 #U +17 "syringe1"Ce& +3 "core" &߆ 7 #X +6 "syringe1"Cm% +3 "core" %6ɱE +8 "syringe1"C7& +4 "core" ÷& 9p #c +16 "syringe1"CV& +4 "core" &I ;B #v +16 "syringe1"Cs& +3 "core" & =3 #s +5 "syringe1"C_% +3 "core" %6ɪT +9 "syringe1"CK& +3 "core" &  ?  # +17 "syringe1"Cw& +3 "core" &n @ # +17 "syringe1"CO& +3 "core" & B2 # +4 "syringe1"C% +3 "core" Â%6 ɫ +11 "syringe1"CP& +5 "core" &@ Do # +14 "syringe1"CZ& +3 "core" & #F # +17 "syringe1"C\\& +3 "core" & H^4 # +8 "syringe1"C% +4 "core" Ç%6ɵ +3 "syringe1"Cz% +4 "core" %6ɨ +3 ."pump"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +7 ."pump"Air.seek :pressurized +3 "syringe1"Cn' +7 CG& +4 "core" &)L # +4 "syringe1"C{& +7 "core" &9M׊ # +6 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 419uL during Lysis Syringe #1 (LyS1) move: @volumef418.62857142857138:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +4 .Lysis1.in :default +4 "syringe1"C?d P[<2 +7 ."PROCESSING"Lysis1.seek 389uL +4 "syringe1"C (N +8 "core" â(OP0 # +131 É( +3 "syringe1"C1% +4 "core" ñ%f[ j< +437 ' +2 "syringe1"Cy% +5 "core" %6S܋q < +2 #"pump"13.8psi after pressurize +3 .Air.in :compressLysate +3 "syringe1"C>dN  P]z<2 +7 ."pump"Air.seek :evac +2 "syringe1"C'| +435 "core" Ä';N;׌ < +4 "syringe1"Ck% +4 "core" %SNNی < +3 #"pump"Reached 15.5 of 15.5psi after pumping 1468uL (0.83 strokes) +2 .Air.in :slow +4 "syringe1"C>d P]z<2 +7 ."pump"Air.hold +3 "syringe1"C\ + +4 "core" Ê+ +6 "syringe1"C% +3 ."PROCESSING"Lysis1.in :extract +5 "syringe1"C?d 'FJ P[#2 +6 "core" Õ%NG +5 ."PROCESSING"Lysis1.seek 675uL,nil +2 "syringe1"C( +9 C|& +4 "core" &NJB܌ # +4 "syringe1"C& +10 "core" Û&P>JB # +3 "syringe1"C%& +5 "core" å&XJdڍ # +3 "syringe1"C& +5 "core" Ï&_J& # +5 "syringe1"C% +3 "core" Ú%NB& +5 "syringe1"C.& +5 "core" î&wK+ # +15 "syringe1"C& +3 "core" Ý&aK # +67 "syringe1"C#& +3 "core" ã&^O܌- # +5 "syringe1"C% +4 C(& +5 "core" Á%NBٌ( + è&Pb # +4 "syringe1"C)& +2 Cp% +19 "core" é&Qɋ # + %N7⋭ +8 ."pump"Air.in :compressLysate +3 "syringe1"C>dN  P]z<2 +7 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 461uL during Lysis Syringe #1 (LyS1) move: @volumef461.38412698412696%b:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6 ."pump"Air.seek :evac +10 ."PROCESSING"Lysis1.in :default +6 "syringe1"C,'| +3 C?d P[<2 +11 ."PROCESSING"Lysis1.seek 431uL +3 "syringe1"C( +11 "core" (9XW#n #w +114 ì'PEL d< +4 "syringe1"C&% +6 "core" æ%PL d< +3 #"pump"Reached 16.2 of 15.5psi after pumping 1487uL (0.84 strokes) +5 .Air.in :slow +2 "syringe1"C>d P]z<2 +6 ."pump"Air.hold +4 "syringe1"C'+ +7 "core" ç+ +2 Æ( +2 "syringe1"Ct% +3 C% +16 "core" %PL̍ +2 Ð%PL̍ +4 ."PROCESSING"Lysis1.in :extract +2 "syringe1"C?d 'FJ P[#2 +8 C& +6 ."PROCESSING"Lysis1.seek 675uL,nil +3 "syringe1"C( +9 "core" È&M] +3 "syringe1"C& +5 "core" Ò&7Mԍ # +3 "syringe1"Cx& +9 "core" & MՍ # +5 "syringe1"Cq% +4 "core" %PL؍ +4 "syringe1"C & +4 "core" Ì&(HNٍ # +8 "syringe1"C & +3 "core" Í&DOݍ # +17 "syringe1"C& +3 "core" Ø&HP\\ލn # +14 "syringe1"C$% +4 "core" ä%PL֍) +4 "syringe1"C2& +3 "core" ò&Rk # +12 "syringe1"C+& +6 "core" ë&9T׌ # +14 "syringe1"CL& +3 "core" & Uڌ5 #z +11 "syringe1"C % +3 "core" à%PL֋ +4 "syringe1"C% +4 "core" Ó%PL +4 ."pump"Air.in :compressLysate +5 "syringe1"C>dN  P]z<2 +8 CB& +2 ."pump"Air.seek :evac +6 "syringe1"C5'| +9 "core" &y.Zڋ1 #S +3 "syringe1"Cv& +4 "core" &;\\  #M +3 "syringe1"C0& +5 "core" ð&M\\p #O +8 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 596uL during Lysis Syringe #1 (LyS1) move: @volumef595.99365079365077:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +8 .Lysis1.in :default + "syringe1"C?d P[<2 +8 ."PROCESSING"Lysis1.seek 566uL +3 "syringe1"C3(A +6 "core" Î(~c8_ #@ +130 ó( +3 "syringe1"CU% +4 "core" %4Jd e< +190 õ'j4H#Ì < +5 "syringe1"C~% +4 "core" %j,Hό < +2 #"pump"Reached 15.7 of 15.5psi after pumping 1541uL (0.87 strokes) +3 .Air.in :slow +2 "syringe1"C>d P]z<2 +10 ."pump"Air.hold +2 "syringe1"C + +9 "core" Ë+ +2 "syringe1"C8% +3 ."PROCESSING"Lysis1.in :extract +7 "syringe1"C?d 'FJ P[#2 +7 ."PROCESSING"Lysis1.seek 675uL,nil +3 "syringe1"C4( +6 Cc& +2 "core" ø%jH +2 &AY* #S +5 "syringe1"CN& +4 "core" &C7YӍ( #X +3 "syringe1"C:& +4 "core" ú&FVY܍% #] +5 "syringe1"C?& +4 "core" ÿ&LYӍ7 #^ +16 "syringe1"C@& +4 "core" &eZ+ԍ$ #^ +9 "syringe1"C6% +3 "core" ö%jHэ +5 "syringe1"C!& +3 "core" á&]Zԍ #\\ +17 "syringe1"C& +3 "core" Ù&[Ҍ #Y +16 "syringe1"C-& +4 "core" í&\\ьz #I +6 "syringe1"C>% +3 "core" þ%jHҌV +3 "syringe1"CJ% +4 "core" %jH +4 ."pump"Air.in :compressLysate +4 "syringe1"C>dN  P]z<2 +7 ."pump"Air.seek :evac +4 "syringe1"C/'| +10 Cb& +3 "core" &*S_͊ ~#A +4 "syringe1"CR& +6 "core" &B`h% ~#G +6 !"PROCESSING"o: Syringe::LysatePlungerStuck : mesg"3at < 642uL during Lysis Syringe #1 (LyS1) move: @volumef641.80317460317451l:@checkpoint: omitted:bt["M/home/esp/esp2/protocol/3G/function/LyseGo.rb:238:in `recoverMoreLysate'"4/home/esp/esp2/lib/thread.rb:879:in `exclusive'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:237:in `recoverMoreLysate'"@/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `loop'"M/home/esp/esp2/protocol/3G/function/LyseGo.rb:208:in `recoverMoreLysate'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +4 .Lysis1.in :default +4 "syringe1"C?d P[<2 +8 ."PROCESSING"Lysis1.seek 612uL +4 "syringe1"CS( +7 "core" ô(BbL% }#B +84 ï'OF e< +5 "syringe1"CF% +8 "core" %BF d< + ( +2 #"pump"Reached 15.6 of 15.5psi after pumping 1560uL (0.88 strokes) +4 "syringe1"C*% +3 ."pump"Air.in :slow +6 "syringe1"C>d P]z<2 +6 "core" ê%#FŒ +3 ."pump"Air.hold +4 ."PROCESSING"Lysis1.in :extract +12 "syringe1"C+ +7 C?d 'FJ P[#2 +60 CQ& +13 ."PROCESSING"Lysis1.seek 675uL,nil +8 "syringe1"CD( +6 "core" Ô+ + &]jA A +3 "syringe1"C% +6 C& +8 "core" ß%FȍW + Ü&O]jM #P +4 "syringe1"C& +7 "core" Å&]ݍl #M +3 "syringe1"C}& +4 "core" &]͍] #D +5 "syringe1"CW& +4 "core" &)^$a #H +16 "syringe1"C=& +4 "core" ý&S^ƍ #D +7 "syringe1"CI% +4 "core" %Fƍ +5 "syringe1"CH& +4 "core" &_Ȍ #G +16 "syringe1"C& +4 "core" Ö&a7ƌ" #B +17 "syringe1"C<& +3 "core" ü&$bË #: +4 ( +4 "syringe1"CC& +4 "core" &bċ #? +2 "syringe1"C% +4 "core" Þ%Fc +3 #"PROCESSING"Pulled 675uL lysate @14.4psi +3 "valve0"D^% +7 "core" %B(OS +5 "syringe1"Cu% +8 ."PROCESSING"Lysis1.seek 800uL,nil +3 "syringe1"CY(% +6 "core" %F +3 "syringe1"CT% +8 C[& +2 "core" %FɌ +5 ."pump"Air.in :compressLysate +2 "syringe1"C>dN  P]z<2 +8 ."pump"Air.seek :evac +3 "syringe1"Cj'| +5 "core" &bČ #3 +4 "syringe1"Cd& +4 "core" &Mb #: +3 "syringe1"Cg& +5 "core" &Kb ~#7 +5 "syringe1"Ch& +4 "core" &!0b #/ +17 "syringe1"C& +3 "core" ×&(ab0 #: +16 "syringe1"C& +5 "core" Ñ&4Yc #> +17 "syringe1"Cf& +5 "core" &&=8c% #= +13 "syringe1"Ci& +4 "core" &.GScR  #9 +16 "syringe1"Cl& +4 "core" &7LXc} }#; +16 "syringe1"Co& +4 "core" &BhWc ~#5 +18 "syringe1"CA& +3 "core" &QYd #: +15 "syringe1"Ce& +4 "core" &bad] #4 +16 "syringe1"C`& +4 "core" &z[d\ #3 +16 "syringe1"Cm& +4 "core" &Jek }#; +16 "syringe1"C7& +4 "core" ÷&hUfA #/ +16 "syringe1"CV& +4 "core" &egBW ~#% +18 "syringe1"Cs& +5 "core" &3Th ~# +14 "syringe1"C_& +3 "core" &gki # +16 "syringe1"CK& +4 "core" &Lij # +16 "syringe1"Cw& +4 "core" &jk@ # +16 "syringe1"CO& +4 "core" &Gk # +16 "syringe1"C& +4 "core" Â&0al # +16 "syringe1"CM& +4 "core" &Ol  }#\ +16 "syringe1"CP& +4 "core" &8l2  +17 "syringe1"CZ& +5 "core" &=lN  +14 "syringe1"C\\& +4 "core" &Ylb +19 "syringe1"C& +4 "core" Ç&[lo +13 "syringe1"Cz& +4 "core" &Rlu +16 "syringe1"CG& +4 "core" &]ls  +17 "syringe1"C{& +3 "core" &Gl  +17 "syringe1"C1& +3 "core" ñ&Hl +16 "syringe1"C;& +4 "core" û&5l +16 "syringe1"C"& +4 "core" â&_l\  +17 "syringe1"Cy& +3 "core" &Cl +16 "syringe1"Cn& +4 "core" &Ul  \ +16 "syringe1"Ck& +4 "core" &Zl  +17 "syringe1"C& +6 "core" Õ&_l  +14 "syringe1"C|& +3 "core" &:l  +17 "syringe1"C& +3 "core" Û&[lD  +16 "syringe1"C%& +4 "core" å&lی ~ +16 "syringe1"C& +4 "core" Ï&Nl8 ~# +16 "syringe1"C& +4 "core" Ú&tls #\ +16 "syringe1"C.& +5 "core" î&4|lE; |# +16 "syringe1"C& +6 "core" Ý&Idlka }# +13 "syringe1"C#& +4 "core" ã&]xlt |# +16 "syringe1"C& +4 "core" Á&vvlh ~# +18 "syringe1"C(& +5 "core" è&^m-| }# +10 ( +3 ."pump"Air.in :slow +2 "syringe1"C>d P]z<2 +5 ."pump"Air.hold +3 "syringe1"Cp+ +11 "core" 'k;v }<~ + + +2 "syringe1"C)& +6 "core" é&%Omr  +3 "carousel0"F% +4 "core" Ƅ%X}q \ +6 ."PROCESSING"Air.in :glide +2 "syringe1"C>d  P]z<2 +7 ."PROCESSING"Air.seek :fullVent +2 "syringe1"C '݇ +201 "Can"m]B.zs?<uA +52 "core" É' +2 ."PROCESSING"Handoff.select :waste +2 "valve0"D +50 #"PROCESSING"Recovered 800uL lysate @15.6psi +2 .delay 3 seconds +304 "syringe1"C&% +3 "core" æ%݇/r +56 #"PROCESSING"After 3 delay Recovered 800uL lysate @0.4psi +16 .Plunge.seek :locked +5 "valve0"D'Z +127 "core" Đ' +3 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +7 Bt% +3 "core" %{"$q ^ +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 C& +4 "core" È&%mw7 +50 ."Lysis2mixer"Lysis2.seek 700uL +7 ."PROCESSING"Lysis1.seek 550uL +7 "syringe0"B'| +5 "syringe1"Cx( +3490 "core" ’' +6 ( +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +54 ."PROCESSING"SPR.command "start 2 14" +136 #SPR stabilized +2 "syringe1"Cq& +6 "core" &Xk= a +4 "syringe0"B % +9 "core" Œ%|f mD n +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +310 #Waiting up to 11:00 for SPR to enter state 1 +4703 "Can"n\\/zt1{_ +7106 ."PROCESSING"SPR.command "hold_on" +192 #SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B % +7 "core" %|f < o +5 "syringe1"C\ & +4 "core" Ê&X#S Z +60 ."Lysis2mixer"Lysis2.seek 400uL +7 "syringe0"B' +6 ."PROCESSING"Lysis1.seek 250uL +8 "syringe1"C( +4175 "core" ˜' +11 ( +2 ."PROCESSING"delay 15 seconds +366 "Can"n\\/z s0-} +1136 ."PROCESSING"SPR.command "hold_off" +371 #Waiting up to 1:00:00 for SPR to enter state 7 +10446 "Can"m\\g/zt. +11953 l\\.zt/O +11971 l]1.zt-L +11942 l].zt0D +11957 n\\.zt/8 +11956 n\\.zt/1 +11954 m]|.zt/y*O +11953 j\\.zt/m +3373 #"SPRlogger"SPRsummary:1.43RIU,65.1RIU,62.8RIU,99.9RIU,134ng/L,139ng/L,154ng/L after 3489s +8580 "Can"m]A.zt%h +11953 n].zt%i +11954 m]M.zt% +11952 m]F.zt+ d +11954 m\\.zt% @EDT1785556851.65 l]".zt& c<! +11954 l]x.zt+ ? +5388 #"PROCESSING"SPR analysis completed +630 #"SPRlogger"Ended log spr26jul3123h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +71 "carousel0"F$% +4 "core" Ƥ%X}s \ +7 #"detached from preston#2"Selecting Cartridge 45 +2 "carousel0"F2% +11 "core" Ʋ%X}| \ +3 "valve0"D+% +4 "core" ī%ZGGOe E +5 ."detached from preston#2"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +5 "valve1"EL& +5 "core" & N:"FOi | +3 ."detached from preston#2"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from preston#2"PinPort.seek :clear +3 "valve1"E ( +80 "core" Š( +2 ."detached from preston#2"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +4 "core" Ɠ%X}K \  +3 ."detached from preston#2"Carousel.home.seek 30 +3 "carousel0"FB'X +17 "core" ' +3 "carousel0"Fv% +4 "core" %X.} 2 +3 "syringe0"B0& +4 "core" °&*r} +5 ."Archive"Archive.in :unlocking +4 "syringe1"C,% +5 "syringe0"B? PZ-2 +6 ."Archive"Archive.seek :clear +3 "syringe0"B(j +9 "core" ì%݇/ +5 ."Air"Air.in :unlocking +3 "syringe0"BU% +4 "syringe1"C> P]z-2 +57 ."Air"Air.seek :clear +4 "syringe1"C'' +6 "core" %LXz +7 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +5 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B'j +360 "core" †( +24 Ž' +56 ç' +3 "syringe1"C~& +3 "core" &>T | +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"D8% +6 "syringe1"C? P[(2 +5 "core" ĸ%ZG%+OS 7 +3 ."Lysis1"Lysis1.seek :clear +6 "syringe1"Cc( +4 ."Plunge"Plunge.in :unlocking +5 "valve0"D> P ޤ(2 +8 ."Plunge"Plunge.seek :clear +2 "valve0"DN'8 +98 "core" ' +300 ( +4 ."detached from preston#2"Carousel.seek 45 +6 "carousel0"F:'+ +332 "core" ƺ' +4 "carousel0"F?% +4 "core" ƿ%+; 2 +3 ."detached from preston#2"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"C@& +5 "core" &غOy +3 ."detached from preston#2"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from preston#2"Lysis1.seek :locked +2 "syringe1"C6( +251 "core" ö( +4 "valve0"D!% +6 "core" ġ%8[!O +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +6 ."Plunge"Plunge.seek :locked +3 "valve0"D'Z +5 "syringe1"C-% +7 "core" í%ݞI +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +5 ."Air"Air.seek :locked +4 "syringe1"C>'6 +70 "core" ę' +34 þ' +3 ."detached from preston#2"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +12 FJ% +3 "core" %+Z  +10 #"detached from preston#2"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from preston#2"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +10 ."detached from preston#2"Gate.power :cartridge,:OFF +5 #Mounted archive Cartridge +8 "valve0"Db% +3 "core" %ZGTFOb  +3 "syringe1"CR& +3 "core" &F~ s +3 "syringe1"C5% +4 "core" õ%6L L +3 "carousel0"FF% +3 "core" %+w \ +4 #"FILTERING"Preparing to filter archiveHiBiomass_bac cartridge in slot 45 +3 "valve0"D3% +8 "core" ij%ZGBO]  +2 "syringe1"C*& +4 "core" ê&N m +3 "syringe1"CQ% +5 "core" %6Mt D +3 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +7 "core" ǟ& +43 "carousel1"G& +4 "core" ǜ&}8 +46 "carousel1"G & +3 "core" Nj& +47 "carousel1"G& +4 "core" Dž& +46 "carousel1"G}& +4 "core" &Z +46 "carousel1"GW& +3 "core" &` +47 "carousel1"G4& +3 "core" Ǵ& +47 "carousel1"G=& +4 "core" ǽ&\ +47 "carousel1"GI& +3 "core" & +47 "carousel1"GH& +4 "core" &0 +46 "carousel1"G& +4 "core" ǖ& +46 "carousel1"G<& +3 "core" Ǽ&H +47 "carousel1"GC& +3 "core" & +47 "carousel1"G& +4 "core" Ǟ& +5 "Can"l]x.ztlmV + #"FILTERING"Intake Head Pressure tare is 0% of full scale at 29C (drifted 6%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +7 .PhaseSeparator.in :filling + "carousel1"G?!! +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G^, +5 "core" , +3 ."FILTERING"Gate.power :sampler,:ON +664 "core" ,d +7 #"FILTERING"Head pressure reached 3.95psi in 7 seconds +2 "carousel0"Fu% +6 "core" %+d \ +3 #"FILTERING"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +5 ."FILTERING"Bypass.open +2 "sampler1"IT1 +152 "core" 1 +74 "ambient0"J/% +3 "core" ʯ% +3 ."FILTERING"Intake.open +2 "sampler0"H[1 +142 "core" 1 +2 "ambient0"Jd% +5 "core" % +3 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +53 "sampler0"Hg% +13 "core" %a] +38 "sampler0"Hh% +5 "core" %Wr +45 "sampler0"H% +3 "core" ȗ% +47 "sampler0"H% +4 "core" ȑ%P +46 "sampler0"HS% +3 "core" %J +47 "sampler0"Hf% +3 "core" %} +6 "Can"l]x.zsDgZI + #"FILTERING"Vacuum tare is 54% of full scale at 29C (drifted 2%) +3 "ambient0"Ji% +6 "core" % +3 "ambient0"Jl% +3 "core" %l +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"Jo+ +6 "core" + +2 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G?3 +6 ."FILTERING"PhaseSeparator.hold +3 "carousel1"GA, +5 "core" , +4 "sampler0"He% +5 "core" %~ +3 "sampler0"H`% +5 "core" % +2 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "00:05:22.88" +1317 "Can"n]/.zsCT] +3220 n]/.zsCue +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }o#(@R3 22Q +9 ."FILTERING"SamplePump.seek 10ml,1:10 +3 "sampler0"Hm'6 +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }o#(@R3 22Q +1285 "core" ' +2 "sampler0"H7% +6 "core" ȷ%6 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +6 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Exhaust.open +3 "sampler0"HD2 +149 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"IV/ +150 "core" / +73 "sampler0"Hs% +3 "core" %U +3 "ambient0"J% +4 "core" ʔ% +3 ."FILTERING"Abs.in :default +3 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J_+ +6 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GK, +7 "core" , +2 "ambient0"Jw% +5 "core" % +3 "sampler0"HO% +5 "core" %+ +3 "sampler0"H% +5 "core" Ȃ%< +5 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }o#(@mR3 22Q +9 ."FILTERING"SamplePump.seek 200ml,13:50 +3 "sampler0"HM'G +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }o#(@mR3 22Q +6745 "Can"l].zs:[/{ +11956 k\\a.zs?[ +7493 "core" ' +2 "sampler0"HP% +4 "core" %G 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +4 ."FILTERING"PhaseSeparator.stop +4 "carousel1"G6 +9 ."FILTERING"Bypass.open +3 "sampler1"IZ1 +151 "core" 1 +2 ."FILTERING"Intake.close +2 "sampler0"H\\/ +142 "core" / +3 "carousel0"F% +5 "core" Ƈ%+4d \ +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :crack +2 "valve0"Dz( +171 "core" ( +77 ."FILTERING"Toroid.seek :cartridge +3 "valve0"DG(m +208 "core" ( +5 ."FILTERING"Toroid.in :default +3 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :clear +3 "valve0"D{( +372 "core" ( +2 "ambient0"J1% +4 "core" ʱ% +4 "Can"k\\a.zshL +2 "ambient0"J;% +4 "core" ʻ%" +2 "ambient0"J"% +5 "core" ʢ%2 +2 #"FILTERING"Pressurizing cartridge to 26psi above 15.6psia +3 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Intake.open +5 "sampler0"Hy1 +142 "core" 1 +2 ."FILTERING"Bypass.close +3 "sampler1"In/ +148 "core" / +3 ."FILTERING"Exhaust.close +2 "sampler0"Hk0 +151 "core" 0 +3 "sampler0"H% +5 "core" ȕ% +2 "ambient0"J|% +4 "core" % +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +50 "ambient0"J+ +5 "core" ʛ+ +3 ."FILTERING"PhaseSeparator.hold +12 "carousel1"G%, +8 "core" ǥ, +3 "sampler0"H% +4 "core" ȏ%I +3 "sampler0"H% +5 "core" Ț%S +4 ."FILTERING"SamplePump.in :charge + "sampler0"H>ou03 22Q +9 ."FILTERING"SamplePump.seek 2.3ml,40 seconds +2 "sampler0"H.' +1405 "core" Ȯ'#S 2 +4 "sampler0"H% +6 "core" ȝ%P 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +6 ."FILTERING"Intake.close +2 "sampler0"H#/ +147 "core" ȣ/ +2 "ambient0"J% +4 "core" ʁ% +2 #"FILTERING"Pressurized cartridge with 0.836ml to 29.5psi +2 #Isolated @36.5psia (20.8psi) +5 #Verifying cartridge holds at least 0.65..0.85 of 36.5psia +6 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +5 "ambient0"J(% +4 "core" ʨ% +47 "ambient0"J)% +4 "core" ʩ% +46 "ambient0"J% +3 "core" ʄ% +47 "ambient0"Jj% +3 "core" % +47 "ambient0"Jp% +3 "core" % +47 "ambient0"J&% +3 "core" ʦ% +47 "ambient0"Jt% +4 "core" % +46 "ambient0"J% +3 "core" ʈ% +47 "ambient0"J9% +4 "core" ʹ%h +46 "ambient0"Jr% +4 "core" %b +2 #"FILTERING"Cartridge pressure fell 1% to 36.3psia +2 "carousel0"FY% +8 "core" %+e \ +3 #"FILTERING"Sampling 1000.0ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"Hq2 +151 "core" 2 +2 ."FILTERING"Intake.open +3 "sampler0"H 1 +138 "core" Ȍ1 +5 "Can"k\\a.zs\\O, + "ambient0"J % +4 "core" ʍ%&J +5 "ambient0"J\ % +4 "core" ʊ%&E +3 ."FILTERING"Abs.in :default +2 "ambient0"J>: e  +7 ."FILTERING"Abs.hold +2 "ambient0"J+ +5 "core" ʒ+ +4 ."FILTERING"PhaseSeparator.hold +2 "carousel1"Gx, +8 "core" , +2 "ambient0"J$% +4 "core" ʤ%!8 +4 "sampler0"H2% +5 "core" Ȳ%׊8  +2 "sampler0"H+% +4 "core" ȫ%܊  +8 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ o#(@m@3 22Q +8 ."FILTERING"SamplePump.seek 1000ml,1:07:10 +3 "sampler0"HL'g+ +56 ."pumpStarted"SamplePump.configure :sample +2 "sampler0"H#d o#(@m@3 22Q +94 "Can"k\\.zs@[Q +132 "sampler0"H% +4 "core" ȓ%\ * z2 +3 "ambient0"Jv% +4 "core" % y +2 #"sampling"Cartridge 45 filtering 2/1000ml at 0.76ml/s with 17.0psi vacuum +5993 "sampler0"H0% +4 "core" Ȱ% /֛ z2 +3 "ambient0"J,% +5 "core" ʬ%1 z +2 #"sampling"Cartridge 45 filtering 47/1000ml at 0.75ml/s with 21.8psi vacuum +5800 "Can"i\\.zs@[ +186 "sampler0"HU% +3 "core" % 5ƙI {2 +3 "ambient0"Ja% +3 "core" % y +3 #"sampling"Cartridge 45 filtering 91/1000ml at 0.74ml/s with 18.9psi vacuum +5988 "sampler0"HX% +4 "core" % ? y2 +2 "ambient0"J% +4 "core" ʃ%$ x +3 #"sampling"Cartridge 45 filtering 135/1000ml at 0.72ml/s with 20.9psi vacuum 4.0m deep (20.6psia) +5756 "Can"m].zsKZ[ +231 "sampler0"H % +3 "core" ȉ%x 5ϗ y2 +3 "ambient0"J% +3 "core" ʘ% y +2 #"sampling"Cartridge 45 filtering 177/1000ml at 0.71ml/s with 17.2psi vacuum +5989 "sampler0"H~% +4 "core" % +ϗ |2 +3 "ambient0"J8% +4 "core" ʸ% x +2 #"sampling"Cartridge 45 filtering 219/1000ml at 0.69ml/s with 16.1psi vacuum 4.9m deep (21.9psia) +5712 "Can"j\\/zs>\\3 +275 "sampler0"H% +3 "core" % 5 y2 +3 "ambient0"J% +4 "core" ʐ% x +2 #"sampling"Cartridge 45 filtering 258/1000ml at 0.66ml/s with 23.7psi vacuum 3.9m deep (20.5psia) +5988 "sampler0"HB% +3 "core" %L2\ ,~ {2 +3 "ambient0"J?% +4 "core" ʿ% y +2 #"sampling"Cartridge 45 filtering 294/1000ml at 0.60ml/s with 40.8psi vacuum +5665 "Can"l\\Q/ysBZ / +323 "sampler0"H@% +3 "core" %` D |2 +3 "ambient0"J!% +4 "core" ʡ% z +2 #"sampling"Cartridge 45 filtering 327/1000ml at 0.55ml/s with 22.2psi vacuum +5988 "sampler0"H-% +3 "core" ȭ%H⚪ {2 +3 "ambient0"Jc% +4 "core" %! z +2 #"sampling"Cartridge 45 filtering 355/1000ml at 0.47ml/s with 20.6psi vacuum +5620 "Can"m\\/z\ sMXNM +368 "sampler0"HJ% +3 "core" % }2 +3 "ambient0"JN% +4 "core" %+I | +2 #"sampling"Cartridge 45 filtering 379/1000ml at 0.41ml/s with 33.9psi vacuum +5988 "sampler0"H'% +3 "core" ȧ%ԗ ~2 +50 "ambient0"Jb% +4 "core" %4 | +2 #"sampling"Cartridge 45 filtering 399/1000ml at 0.34ml/s with 16.9psi vacuum 5.6m deep (23.0psia) +5529 "Can"j\\/z\ sPW"k +412 "sampler0"HR% +3 "core" %j |2 +3 "ambient0"J5% +5 "core" ʵ% | + #"sampling"Cartridge 45 filtering 416/1000ml at 0.28ml/s with 39.8psi vacuum +5988 "sampler0"HF% +4 "core" % :U ~2 +2 "ambient0"J3% +5 "core" ʳ% ~ +2 #"sampling"Cartridge 45 filtering 431/1000ml at 0.25ml/s with 19.9psi vacuum 3.9m deep (20.5psia) +7 "sampler0"H*% +5 "core" Ȫ% :v ~2 +3 ."FILTERING"SamplePump.stop +2 "sampler0"H5 +7 ."closeIntake"Intake.close +4 "sampler0"HQ/ +6 ."closeExhaust"Exhaust.close +9 ."closeHandoff"Handoff.select :close +5 "sampler0"H0 +4 "carousel0"F% +5 "valve0"D +13 "core" ' :' 2 + Ɯ%+h< \ +5 ."FILTERING"Toroid.in :hold +3 "valve0"D? ֜2 +6 ."FILTERING"Toroid.seek :bypass +4 "valve0"D ( +88 "core" / +21 ȟ0 +245 ċ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +8 ."FILTERING"Toroid.seek :clear +5 "valve0"D( +353 "core" ą( +2 !"FILTERING"o: Cartridge::Sampler::Stuffed: mesg"Cartridge clogged:@checkpoint: omitted:bt[ "d P]z<2 +6 ."PROCESSING"Air.seek :sealed +3 "syringe1"C' +141 ."sourcePumpShutdown"Gate.power :sampler,:OFF +1077 "core" Þ' +2 ."PROCESSING"Air.seek :arEvac +3 "syringe1"Cu'< +3285 "Can"k\\@/ys?+ +4172 "core" ' +4 ."PROCESSING"delay 1:00 +6005 .Air.seek :clear +2 "syringe1"CT' +1775 "Can"k\\.z s?0 +7943 "core" ' +3 #"PROCESSING"RNAlater soak for 10:00 +4012 "Can"m\\.ztH>7 +11960 l]?.ztGV9 +11962 n]7.ztFL< +11958 j]K.ztI>? +11957 l].ytGB= +8155 ."PROCESSING"Air.seek :tVent +3 "syringe1"C/'6 +1070 "core" ï' +2 ."PROCESSING"delay 1 second +103 .Air.in :halfSpeed +2 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.seek :arEmpty +2 "syringe1"C['< +2612 "Can"l]K.z s?G[ +655 "core" ' +2 ."PROCESSING"delay 10 seconds +1004 .delay 1:00 +6004 .Plunge.seek :pTop +2 "valve0"Dd': +10 "core" ' +4 #"PROCESSING"Sample preserved and evacuated +2 "carousel0"Fg% +6 "core" %+v/ \ +12 "sampler0"Hh% +4 "core" % :B +10 "valve1"E& +3 "core" ŗ&OX q +3 ."detached from preston#2"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" Ƒ%+5 \ +3 ."detached from preston#2"Carousel.home.seek 45 +3 "carousel0"FS'+ +17 "core" ' +2 "carousel0"Ff% +5 "core" %+X  +3 "syringe1"Ci& +3 "core" &z+ z +3 ."detached from preston#2"Plunge.seek :locked +3 "valve0"Dl'Z +87 "core" ' +2 "syringe0"Be& +4 "core" &j&6 +3 "syringe1"C`% +4 "core" %<.% +5 ."Air"Air.in :unlocking +3 "syringe1"C^& +3 C> P]z-2 +9 ."Air"Air.seek :clear +3 "syringe1"CE' +5 "core" &D p +10 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B7% +2 "syringe1"C? P[(2 +9 ."Lysis1"Lysis1.seek :clear +3 "syringe1"CD( +5 "core" ·%jٷҁ9 +126 ( +303 ' +3 "sampler0"Ho% +4 "core" % :A +10 ."detached from preston#2"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +50 .Gate.power :core,:OFF +10 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785559207.71EDT @EDT1785559242.79 "Can"n]W.ytIy +11956 n]`.ztJS +11958 m]j.z tJ +11960 l].z tKk +11957 n].ztK +11959 n].ztL +11959 k]N.ztM- +11957 m^.z tMK +11959 m].ztN(i +11962 l^0.ytN +11960 n].z tO@ +11960 m^.ytO +11959 l].ztPY +11954 m^.z tP +11960 m].z tQr  +11958 k^C.z tQ ; +11957 m^2.ytR Y +11958 l^.ztS w +11961 l^x.ztS +11957 m^!.ytT- +11958 m^.ztT +11961 m^E.ztUF +11963 k^t.ytU! +11960 m^.ytV^!+ +11962 m_0.ytV!I +11963 m^.ytWv!g +11960 m^.ytX! +11960 k_.ytX! +11955 m^.ytY! +11956 m_[.ytY! +11958 j_.ztZ4! +11957 l_$.z\ tZ" +11959 m_e.yt[L"9 +11958 l_B.yt["W +11957 l_.yt\\d"u +11958 m_l.yt\\" +11959 k_.yt]~" +11955 i_}.}yt^\ " +11957 l_.uyt^" +11959 l_.tyt_"# +11955 m`I.ryt_#) +11959 m`.nyt`:#G +11959 l_.lyt`#e +11958 m`.lztaS# +11957 k`.izta# +11956 k`.gytbj# +11958 l`.cztb# +11960 o`S.`ytc# +11959 n_.dytd$ +11957 m`%.aytd$7 +11957 l`x.`yte)$U +11960 n_.^yte$s +11959 n`P.VytfB$ +11957 l_.Uytf$ +11959 m`.PytgZ$ +11957 n`.Pytg$ +11959 l`.Lyths% +11959 m`.Lyth%' +11959 l`.Lyti%E +11958 m`.Eytj%c +11956 m`H.Bytj% +11956 m`.Dytk0% +11957 l`.Aytk% +11957 k`.@ytlI% +11956 na.=ytl% +11957 oa_.:ytmb& +11960 l`./w +11961 ldO-yt/ +11960 lc<-ytX/ +11957 lcd-yt/ +11960 md8-ytq/ +11959 od-yt0 +11961 nc-yt0+ +11961 oc-yt0I +11961 md-yt0g +11964 mcs-yt00 +11963 nd!-yt0 +11965 nd-ytI0 +11963 ld.-yt0 +11962 ld-yta0 +11962 ld<-yt1 +11966 md-ytz19 +11964 md-yt1W +11959 kdu-yt1u +11961 kd[-yt1 +11961 ld-yt1 +11960 me -yt71 +11960 ne-yt1 +11961 ne-ytP2 +11959 md-yt2) +11957 ne-ytj2G +11959 le-yt2e +11957 nd-yt2 +11960 ld-yt2 +11959 ld-yt2 +11956 leN-yt(2 +11957 ne-yt2 +11958 med-ytA3 +11959 lft-yt37 +11961 le-yt[3U +11960 lfM-yt3s +11959 meT-ytu3 +11959 me-yt3 +11959 leh-yt3 +11958 me-yt3 +11959 mf%-yt4 +11961 mfK-yt54' +11960 nf-yt4E +11960 nf-ytM4c +11958 mf-yt4 +11955 ne-ytf4 +11955 kgH-yt4 +11955 mgT-yt4 +11956 ng-yt 4 +11960 nf-yt5 +11958 lf-yt$55 +11958 le-yt5S +11960 me-yt=5q +11960 nf-yt5 +11963 mf-ytV5 +11963 lgK-yt5 +11962 lf-yto5 +11960 le-yt6 +11960 kg+-yt6% +11962 lf-yt6C +11959 lf-yt6a +11962 mf-yt.6 +11960 mf-yt6 +11960 ng-ytG6 +11956 je-yt6 +11960 mf-yt`6 +11962 mfj-yt7 +11964 mf-ytx73 +11962 kgK-yt7Q +11962 mf-yt7o +11963 of-yt7 +11960 of2-yt7 +11959 nf-yt77 +11960 jf -yt7 +11957 ng-ytP8 +11962 mf-yt8# +11961 lf-yti8A +11961 mfA-yt8_ +11965 jg-ytÂ8} +11962 mg-yt8 +11961 ogD-ytĚ8 +11963 mgs-yt'8 +11962 kf-ytŴ8 +11967 mh*-ytA9 +11966 nfB-yt91 +11966 kf|-ytZ9O +11967 nf-yt9m +11963 jf^-yts9 +11963 oe-yt9 +11962 je-ytɌ9 +11960 mf-yt9 +11960 mf-ytʦ: +11960 ngS-yt3:! +11960 me-yt:? +11959 kfb-ytM:] +11956 of-yt:{ +11959 lf-ytf: +11957 pf-yt: +11956 ne-yt: +11959 mf-yt : +11961 oe-ytϗ; +11958 me-yt$;/ +11960 of?-ytб;M +11960 me-yt>;k +11959 kf;-yt; +11961 nfO-ytW; +11961 me-yt; +11958 jea-ytp; +11959 nf"-yt< +11960 ne-ytԊ< +11960 me@-yt<= +11958 ne-ytգ<[ +11956 nf-yt/ ESP.reready! +5 .Gate.power :sampler,:OFF,limit to 3500mA +4 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +237 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +30 "valve0"DP +4 DA +2 "core"  +7 ."preston"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."preston"SamplePump.configure :default +3 "sampler0"H#^ #(22 +9 ."preston"Toroid.configure :default +2 "valve0"D$ ֜2 +8 ."preston"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."preston"Abs.configure :default +2 "ambient0"J#: e  +9 ."preston"PhaseSeparator.configure :full + "carousel1"G$3 +9 ."preston"Archive.configure :default +2 "syringe0"B$d PZ<2 +7 ."preston"Air.configure :slow +3 "syringe1"C#d P]z<2 +6 ."preston"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."preston"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."preston"PinPort.configure :default +2 "valve1"E$ B22 +5 #"preston"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +9 ."Plunge_home"Plunge.home.jog 0 +5 "valve0"DV) +4 ."Toroid_home"Toroid.home.jog 0 +9 "core" ) +2 ."Carousel_home"Carousel.home.jog 0 +13 "valve0"Ds* +17 ."Archive_home"Archive.home.jog 0 +11 "carousel0"F) +3 ."Air_home"Air.in :default +10 "valve0"Dw% +3 ."Lysis1_home"Lysis1.home.jog 0 +9 ."Lysis2_home"Lysis2.home.jog 0 +5 ."PinPort_home"PinPort.home.jog 0 +5 "syringe0"BO* +3 "syringe1"C>d P]z<2 +4 C* +2 "syringe0"Bm) +2 "valve1"EP* +19 ."Air_home"Air.home.jog 0 +10 "syringe1"CZ) +5 "core" * + %TG<OD  +2 Ɣ) * + Â* + ) + * + ) +2 "syringe0"B_% +9 "syringe1"CK& +4 "valve1"E\\& +7 "syringe0"B& +2 "valve0"D]& +13 ."Plunge_home"Plunge.in :homing +6 "syringe1"Cz% +2 "valve0"D> (2 +76 ."Plunge_home"Plunge.jog 1477 +4 "valve0"DG) +6 "core" %xٸgp  + &i+O + &O?  + ‡&zEj_ + &ZOA %ݟx +11 ."PinPort_home"PinPort.in :homing +4 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +5 ."Lysis1_home"Lysis1.in :homing +3 ."Archive_home"Archive.in :homing +5 "valve1"E?2 +4 "syringe0"B> (2 +4 "syringe1"C> -2 +3 "valve0"D?2 +2 "syringe1"C? (2 +10 "syringe0"B? -2 +2 ."PinPort_home"PinPort.jog 1238 +6 ."Lysis2_home"Lysis2.jog 1615 +6 ."Air_home"Air.jog 1428 +5 ."Toroid_home"Toroid.jog 1356 +7 ."Archive_home"Archive.jog 1611 +5 "valve1"E1* +4 ."Lysis1_home"Lysis1.jog 303 +5 "syringe0"B;)O +4 "syringe1"C") +4 "valve0"Dy*L +9 "syringe0"Bn*K +3 "syringe1"Ck*/ +15 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +5 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +4 "valve0"D' +24 "core" * +2 ĕ' +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Plunge_home"Plunge.in :default +4 "syringe1"C82 +3 "valve0"D> P ޤ-2 +8 ."Lysis1_home"Lysis1.seek :home +3 "syringe1"C|( +20 "core" â) +2 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +8 ."Air_home"Air.seek :home +3 "syringe1"C' +7 "core" ( + ») + Ï' + * +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +5 ."Lysis1_home"Lysis1.in :default +5 ."Archive_home"Archive.setRawPosition! 50 +4 ."Air_home"Air.in :slow +4 "syringe0"B72 +4 "syringe1"C?d P[<2 +5 "syringe0"B82 +4 "syringe1"C>d P]z<2 +4 ."Lysis2_home"Lysis2.seek :home +5 "syringe0"B' +5 ."Archive_home"Archive.seek :home +8 "syringe0"BM( +6 "core" ű* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +5 ."PinPort_home"PinPort.seek :home +5 "valve1"E( +8 "core" š' + ( +2 ."Archive_home"Archive.in :default +3 ."Lysis2_home"Lysis2.in :default +3 "syringe0"B?d PZ<2 +4 B>d P\\k<2 +225 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +3 "valve0"D#( +4 "core" ģ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +165 "core" ŝ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +5 "valve1"E& +5 "valve0"D(( +4 "core" Ł&©OB W +7 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."preston"PinPort.seek :clear +2 "valve1"E)( +42 "core" ũ( +296 Ĩ( +2 ."preston"Air.seek :clear +4 "syringe1"C' +7 "core" Ä' +2 ."preston"Lysis1.seek :clear +2 "syringe1"C( +70 "core" Û( +3 ."preston"Lysis2.seek :clear +3 "syringe0"B%'j +76 "core" ¥' +4 ."preston"Archive.seek :clear +3 "syringe0"Bj(j +77 "core" ( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fp% +3 "core" %+U  +3 ."preston"Carousel.home.seek 45 +3 "carousel0"F&'+ +18 "core" Ʀ' +2 "valve0"Dt% +5 "core" %^OX !c +3 "valve0"D% +4 "core" Ĉ%^|OT ! +3 "sampler0"H9% +5 "core" ȹ%F +3 "valve0"Dr& +4 "core" &YON Y +3 "carousel0"FY% +5 "core" %+NM  +3 "ambient0"Jq% +4 "core" %|W +3 "carousel1"G & +4 "core" nj&O +3 "syringe0"B & +3 "core" &j8Rc +3 "syringe1"C\ % +4 "core" Ê%ݧG +3 "syringe1"C$& +4 "core" ä&ٔǀP +3 "syringe0"B2% +5 "core" ²%jټPf +2 "valve1"E+& +5 "core" ū&8ON  +139 #"preston"-> SC.coast +4 .Carousel.coast + "carousel0"F5 +5 F% +3 "core" Ɠ%+I +7981 "Can"leV-ytMޠ= +11959 lez-ytK.= +5026 #"preston"-> Head.psi +3 "carousel1"Gv& +5 "core" &n +6921 "Can"nc-ytO= +9356 #"preston"-> ESP.reready! +9 ."Plunge_home"Plunge.in :default +5 ."Toroid_home"Toroid.in :default +60 "valve0"D> P ޤ-2 +3 ."Archive_home"Archive.in :default +11 ."Carousel_home"Carousel.in :default +8 "valve0"D? ֜2 +4 ."Air_home"Air.in :default +5 ."Plunge_home"Plunge.home.jog 0 +7 "syringe0"B?d PZ<2 +7 ."Lysis1_home"Lysis1.in :default +5 "carousel0"F><` F 22 +3 ."Lysis2_home"Lysis2.in :default +5 ."PinPort_home"PinPort.in :default +5 "syringe1"C>d P]z<2 +3 "valve0"D.) +4 ."Toroid_home"Toroid.home.jog 0 +6 "syringe1"C?d P[<2 +4 ."Archive_home"Archive.home.jog 0 +10 "syringe0"B>d P\\k<2 +7 ."Carousel_home"Carousel.home.jog 0 +5 "valve1"E? B22 +3 ."Air_home"Air.home.jog 0 +4 "valve0"D0* +5 "core" Į) + "syringe0"B,* +6 ."Lysis1_home"Lysis1.home.jog 0 +11 ."Lysis2_home"Lysis2.home.jog 0 +6 "carousel0"FU) +3 ."PinPort_home"PinPort.home.jog 0 +7 "syringe1"Ca) +2 "valve0"DX% +10 "syringe1"C* +3 "syringe0"B ) +8 "valve1"E* +19 "core" İ* + ¬* + ) + ) %^O` + Ã* + ‰) + Ř* +2 "syringe0"B~% +11 "syringe1"C8& +5 "valve0"D& +4 "valve1"EB& + "syringe1"C?% +4 "syringe0"B& +5 ."Plunge_home"Plunge.in :homing +13 "valve0"D> (2 +6 "core" %xٶK  + ø&ه  + Đ&\\Oe + &/>OV + ÿ% ݫ9 &zA  +8 ."Plunge_home"Plunge.jog 431 +8 ."PinPort_home"PinPort.in :homing +5 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +5 ."Air_home"Air.in :homing +4 ."Lysis2_home"Lysis2.in :homing +4 ."Toroid_home"Toroid.in :homing +5 "valve0"D@) +2 "valve1"E?2 +2 "syringe1"C? (2 +2 "syringe0"B? -2 +7 "syringe1"C> -2 +8 "syringe0"B> (2 +3 "valve0"D?2 +3 ."PinPort_home"PinPort.jog 1227 +13 ."Lysis1_home"Lysis1.jog 1593 +6 ."Archive_home"Archive.jog 1612 +7 ."Air_home"Air.jog 1426 +5 "valve1"E!* +3 ."Lysis2_home"Lysis2.jog 1615 +8 ."Toroid_home"Toroid.jog 1356 +4 "syringe1"C-*9 +4 "syringe0"Bc*L +2 "syringe1"CJ) +6 "syringe0"BN)O +2 "valve0"D'*L +19 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"Db' +26 "core" ' +2 ."Plunge_home"Plunge.in :default +3 "valve0"D> P ޤ-2 +50 "core" í* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe1"C82 +5 "core" ) +3 ."Air_home"Air.setRawPosition! 0 +5 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"C7 +2 CR( +14 ."Air_home"Air.seek :home +4 "syringe1"C5' +7 "core" * + ) + š* + õ' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Archive_home"Archive.setRawPosition! 50 +4 ."Air_home"Air.in :slow +5 "valve1"E8 N +2 "syringe0"B72 +7 B82 + "syringe1"C>d P]z<2 +5 ."PinPort_home"PinPort.seek :home +4 ."Lysis2_home"Lysis2.seek :home +5 ."Archive_home"Archive.seek :home +6 "valve1"EF( +4 "syringe0"B3' +12 B*( +5 "core" ( +4 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +6 "core" ³' +3 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +9 "core" ª( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +186 "core" ħ* +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D( +6 "core" Ĝ( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +181 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"EQ& +3 "valve0"D( +5 "core" &²OO N +8 ."preston"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."preston"PinPort.seek :clear +3 "valve1"EL( +42 "core" ( +295 ğ( +3 ."preston"Air.seek :clear +4 "syringe1"C{' +6 "core" ' +3 ."preston"Lysis1.seek :clear +3 "syringe1"C ( +69 "core" Ë( +4 ."preston"Lysis2.seek :clear +4 "syringe0"B}'j +75 "core" ' +3 ."preston"Archive.seek :clear +8 "syringe0"BW(j +75 "core" ( +2 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 F4% +4 "core" ƴ%`[_BW[  +3 ."preston"Carousel.home.seek 23 +3 "carousel0"F=' +28 "core" ƽ' +2 "valve0"DI% +5 "core" %^OM !P +2 "valve0"DH% +5 "core" %^OJ !F +3 "sampler0"H% +5 "core" Ȗ%$ +3 "valve0"D<& +4 "core" ļ&?OF s +3 "carousel0"F% +4 "core" ƒ%^_  +3 "ambient0"Jx% +4 "core" %q) +3 "carousel1"G& +4 "core" Ǚ&_` +3 "syringe0"B>& +4 "core" ¾&j=# +3 "syringe1"C% +5 "core" Þ%ݬG +2 "syringe1"Cu& +4 "core" &ـ' +3 "syringe0"BT% +4 "core" %jٰ@ +3 "valve1"E/& +4 "core" ů&UOE  +697 "Can"ne(-yt;3> +113 #"preston"-> delay 5;to 30 +3 .delay 5 seconds +505 "valve0"Dg% +4 "core" %^OK !M +2 "syringe1"Ch& +5 "core" &7 +2 "syringe1"C% +4 "core" ×%ݰր/ +3 "valve1"E& +4 "core" ő&SOL  +3 ."preston"Carousel.seek 30 +3 "carousel0"Ff'X +168 "core" ' +4 "carousel0"Fi% +3 "core" %X?} 2 +3 ."preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"Ce& +3 "core" &w}F +4 ."preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."preston"Lysis1.seek :locked +3 "syringe1"C`( +249 "core" ( +3 "valve0"D^% +6 "core" %^O !A +3 ."Plunge"Plunge.seek :locked +2 "valve0"D7'Z +8 "syringe1"CC% +7 "core" %ݪt +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C:'6 +78 "core" ķ' +26 ú' +3 ."preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 Fd% +3 "core" %Xa}{  +6 #"preston"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."preston"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +6 ."preston"PuckHeater.configure :default #ambient 28.8C +2 "Cartridge" \ :D H  +7 ."preston"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" [y +4 "cartridge" yGBGB +3 "Cartridge" 6y  +5 "cartridge" y _S&0J59913 U&0(CJ +3 #"preston"Mounted lysis Cartridge board#J59913 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1401 #-> to 40 +7 "carousel0"Fo% +4 "core" %X}zK \ +4 "valve0"DA% +4 "core" %ZGVOY  +4 ."preston"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +6 "valve1"Ew& +4 "core" &POZ  +3 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +6 F\\% +3 "core" %X}L \ +3 ."preston"Carousel.home.seek 30 +4 "carousel0"F_'X +17 "core" ' +2 "carousel0"FK% +4 "core" %X`}xh  +3 "syringe0"Bz& +4 "core" &j)g> +3 "syringe1"C% +5 "core" Ç%6Ѐ ; +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +3 "syringe0"B]% +6 ."Air"Air.seek :clear +5 "syringe1"Cl' +5 "core" %jٷ.) +90 ' +2 "syringe1"C& +4 "core" Å&&2 +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"DS% +4 "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +2 "syringe1"C( +7 "core" %ZGKOL  +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DD'8 +96 "core" ' +12 Æ( +3 ."preston"Carousel.seek 40 +3 "carousel0"F'& +230 "core" Ǝ' +7 "carousel0"F % +4 "core" Ơ%&>9 2 +2 ."preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"CE& +3 "core" &e^5 +4 ."preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."preston"Lysis1.seek :locked +42 "syringe1"CV( +250 "core" ( +12 "valve0"DP% +8 "core" %8[Oe +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +8 ."Plunge"Plunge.seek :locked +4 "syringe1"Cm% +4 "valve0"DZ'Z +9 "core" %ݮg* +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"Cs'6 +77 "core" ' +27 ' +2 ."preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 FO% +3 "core" %&bK  +7 #"preston"Powering cartridge bus +267 "cartridge_broadcast" ( +3 ."preston"Gate.setIR 1750,5000,0,6 +16 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."preston"PuckHeater.configure :default #ambient 28.8C +2 "Cartridge" \ :D H  +7 ."preston"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" y +4 "cartridge" y RI +4 "Cartridge" Gy  +4 "cartridge" y k`/ C;R c/J59986 +4 #"preston"Mounted lysis Cartridge board#J59986 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +2246 #-> SC.coast +3 .Carousel.coast +2 "carousel0"F5 +6 Fk% +3 "core" %&% +507 #"preston"-> Cart.unlock +6 .Gate.power :heat,:OFF + .Gate.power :cartridge,:OFF +4 "valve1"E"& +5 "core" Ţ&IOu  +3 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +7 F;% +3 "core" ƻ%&L \ +3 ."preston"Carousel.home.seek 40 +2 "carousel0"Fn'& +19 "core" ' +2 "carousel0"F1% +5 "core" Ʊ%&2 2 +2 "syringe0"B& +5 "core" &j.+ +2 "syringe1"Cy% +4 "core" %6Y9 > +5 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +5 "syringe0"B#% +6 ."Air"Air.seek :clear +3 "syringe1"C' +5 "core" £%jٻ9 +90 Ý' +2 "syringe1"Cp& +4 "core" &6 +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"Dt% +4 "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C( +7 "core" %ZG~Of +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"D'8 +96 "core" ď' +12 È( +216 #"preston"-> SC.coastt +6 !o:NoMethodError\ : mesgu:NameError::message1undefined method `coastt' for Carousel:Slide:@timestampu: Time -b: name: coastt: args[:bt["8/home/esp/esp2/lib/slide.rb:459:in `method_missing'"$preston:10:in `topLevelBinding'" SC.coast +2 .Carousel.coast +3 "carousel0"F5 +5 F9% +3 "core" ƹ%& +3901 "Can"le-ytO>; +11956 nd-ytM>Y +11954 md?-ytN>w +9503 #"preston"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +2452 "Can"md-ys;> +5457 #"preston"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +6497 "Can"nc-ytO,> +8220 #"preston"-> Power.sampler :ON +4 .Gate.power :sampler,:ON +1184 #-> 3.times{Sampler.refill} +3 .Bypass.open +3 "sampler1"Ir1 +154 "core" 1 +3 ."preston"Intake.open +2 "sampler0"H|1 +145 "core" 1 +3 ."preston"SamplePump.setPosition! 0ml + "sampler0"H7 +6 HY% +4 "core" % +3 ."preston"SamplePump.seek 30ml +2 "sampler0"Hq'` +2218 "Can"md.ysWT> +1775 "core" ' +2 ."preston"Bypass.close +2 "sampler1"I / +149 "core" Ɍ/ +3 ."preston"Exhaust.open +2 "sampler0"H 2 +148 "core" ȍ2 +3 "sampler0"H\ % +5 "core" Ȋ%`W +2 ."preston"SamplePump.seek 55ml +3 "sampler0"H$'-[ +3337 "core" Ȥ' +2 ."preston"delay 1 second +103 ."closeIntake"Intake.close +4 "sampler0"H2/ +6 ."closeExhaust"Exhaust.close +5 "carousel0"F+% +4 "sampler0"H0 +5 "core" ƫ%&lX +130 Ȳ/ +14 Ț0 +2 ."preston"Bypass.open +3 "sampler1"IM1 +150 "core" 1 +6 ."preston"Intake.open +2 "sampler0"H1 +144 "core" ȓ1 +3 ."preston"SamplePump.setPosition! 0ml + "sampler0"H7 +6 H% +4 "core" Ȕ% +3 ."preston"SamplePump.seek 30ml +3 "sampler0"H&'` +3991 "core" Ȧ' +2 ."preston"Bypass.close +3 "sampler1"Iv/ +150 "core" / +2 ."preston"Exhaust.open +2 "sampler0"HX2 +150 "core" 2 +3 "sampler0"HB% +3 "core" %`Mw +4 ."preston"SamplePump.seek 55ml +2 "sampler0"H~'-[ +1612 "Can"lc:. ysSTY> +1725 "core" ' +2 ."preston"delay 1 second +112 ."closeIntake"Intake.close +5 ."closeExhaust"Exhaust.close +4 "sampler0"H8/ +6 H?0 +4 "carousel0"F% +7 "core" %&dU +132 ȸ/ +11 ȿ0 +2 ."preston"Bypass.open +2 "sampler1"I1 +150 "core" ɐ1 +2 ."preston"Intake.open +2 "sampler0"H1 +144 "core" ȕ1 +4 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 H(% +5 "core" Ȩ%& +2 ."preston"SamplePump.seek 30ml +3 "sampler0"Hj'` +3992 "core" ' +2 ."preston"Bypass.close +3 "sampler1"I/ +147 "core" Ʉ/ +2 ."preston"Exhaust.open + "sampler0"H2 +148 "core" ț2 +2 "sampler0"H%% +4 "core" ȥ%`^ +3 ."preston"SamplePump.seek 55ml +3 "sampler0"H)'-[ +3337 "core" ȩ' +2 ."preston"delay 1 second +103 ."closeIntake"Intake.close +3 "sampler0"H./ +5 ."closeExhaust"Exhaust.close +7 "carousel0"F% +4 "sampler0"H0 +4 "core" Ƙ%&1R +130 Ȯ/ +20 ȃ0 +818 #"preston"-> BACsampler.prime +2 "carousel0"F,% +6 "core" Ƭ%&7S +4 #"preston"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."preston"Bypass.open +2 "sampler1"Ia1 +149 "core" 1 +73 "ambient0"J % +3 "core" ʉ%n +3 ."preston"Intake.open +2 "sampler0"H01 +142 "core" Ȱ1 +2 "ambient0"J@% +4 "core" % +3 #"preston"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H-% +9 "core" ȭ%> +42 "sampler0"HJ% +3 "core" %B +47 "sampler0"H!% +4 "core" ȡ%7 +46 "sampler0"HN% +3 "core" %4 +47 "sampler0"Hc% +3 "core" %p +47 "sampler0"HQ% +3 "core" %m +6 "Can"lc:. ys9 e  +6 ."preston"Abs.hold +2 "ambient0"JF+ +5 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"G4, +7 "core" Ǵ, +4 "sampler0"H5% +4 "core" ȵ%b +3 "sampler0"HI% +4 "core" % +5 ."preston"SamplePump.in :start +2 "sampler0"H>^ }l8#(@2q3 22Q +7 ."preston"SamplePump.seek 10ml,1:10 +3 "sampler0"HH'6 +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }l8#(@2q3 22Q +81 "Can"lc .ys[S? +1204 "core" ' +2 "sampler0"HR% +5 "core" %6 2 +2 ."preston"Abs.stop +2 "ambient0"J5 +5 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +5 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."preston"Exhaust.open + "sampler0"H2 +149 "core" Ȗ2 +74 ."closeBypass"Bypass.close +4 "sampler1"I9 e  +6 ."preston"Abs.hold +3 "ambient0"J+ +5 "core" ʙ+ +3 ."preston"PhaseSeparator.hold +2 "carousel1"G>, +7 "core" Ǿ, +2 "ambient0"J% +4 "core" ʞ% +3 "sampler0"Hu% +4 "core" %x +3 "sampler0"HT% +5 "core" %v +9 ."preston"SamplePump.in :start +2 "sampler0"H>^ }l8#(@q3 22Q +6 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"H/'G +58 ."pumpStarted"SamplePump.configure :prime + "sampler0"H#d }l8#(@q3 22Q +6606 "core" Ǿ,} +5 "sampler0"H3% +5 "core" ȳ%u #p 2 +2 ."preston"SamplePump.stop +2 "sampler0"H5 +7 "carousel1"G*& +5 "core" ȯ' +叔 2 Ǫ&M +2 !"preston"o:)Cartridge::Sampler::LostHeadPressure: mesg""Head Pressure Fell to 0.73psi:bt["?/home/esp/esp2/utils/cartridge/sampler.rb:698:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'"$preston:16:in `topLevelBinding'" BACsampler.prime +2 "carousel0"Fg% +5 "core" %&Y +4 #"preston"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."preston"Exhaust.close +2 "sampler0"Hh0 +149 "core" 0 +4 ."preston"Bypass.open +8 !o:UserAbort: mesg"deferring User Abort!!:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'",preston aborter-:1:in `topLevelBinding'"Bypass (PB) could not finish opening because\ User Abort!!:@timestampu: Time -k:@checkpoint: omitted:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'",preston aborter-:1:in `topLevelBinding'" Power.sampler :OFF +4 .Gate.power :sampler,:OFF +291 #-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +11352 "Can"lb!.%ytO?I +11957 jc.$ytO?g +11956 mb&.!ytK4? +11953 nbM.&ytM\ !? +2644 #"preston"-> Power.sampler :ON +2 .Gate.power :sampler,:ON +821 #-> BACsampler.prime +3 "carousel0"F% +6 "core" Ƒ%&dP +3 #"preston"Priming sample loop w/200ml, bypass w/10ml +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +5 ."preston"Bypass.open +2 "sampler1"Ii1 +153 "core" 1 +73 "ambient0"Je% +4 "core" % +2 "ambient0"J=% +4 "core" ʽ%b +4 #"preston"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H^% +7 "core" % +43 "sampler0"HC% +3 "core" %U +47 "sampler0"H % +3 "core" ȋ%F +47 "sampler0"Hd% +3 "core" %F +47 "sampler0"Hb% +3 "core" %E +47 "sampler0"H{% +5 "core" %7 +5 "Can"nbM.&ys<#.? + #"preston"Vacuum tare is 53% of full scale at 27C (drifted 1%) +2 "ambient0"J[% +5 "core" % +3 "ambient0"J6% +4 "core" ʶ%! +3 ."preston"Abs.in :default +2 "ambient0"J>9 e  +6 ."preston"Abs.hold +2 "ambient0"Jo+ +5 "core" + +3 ."preston"PhaseSeparator.in :full +2 "carousel1"G?3 +6 ."preston"PhaseSeparator.hold +2 "carousel1"GA, +5 "core" , +4 "sampler0"Hw% +5 "core" %9 +2 "sampler0"H\\% +5 "core" %5 +2 #"preston"Waiting for sampler to finish priming... +2 .delayUntil "10:06:13.04" +389 ."closeIntake"Intake.close +10 ."closeBypass"Bypass.close +5 "carousel0"Fz% +3 "sampler0"HK/ +8 "sampler1"I/ +3 "core" %&pP +144 / +10 ɇ/ +4 ."preston"Abs.stop +2 "ambient0"J5 +6 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +7 !"preston"o:UserAbort : mesg"User Abort!!:@timestampu: Time .d8:@checkpoint: omitted:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'",preston aborter-:1:in `topLevelBinding'" 2.times{Sampler.refill} +2 .Bypass.open +5 "sampler1"I]1 +152 "core" 1 +2 ."preston"Intake.open +3 "sampler0"H1 +143 "core" ȅ1 +2 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 HS% +3 "core" % +3 ."preston"SamplePump.in :default +2 "sampler0"H>^ #(22 +6 ."preston"SamplePump.seek 30ml +2 "sampler0"Hf'` +428 ."preston"SamplePump.hold +11 "sampler0"H:+ +6 "core" '= L 2 +5 Ⱥ+ +3 ."preston"delay 1 second +104 ."closeIntake"Intake.close +5 ."closeBypass"Bypass.close +6 "sampler0"H`/ +6 "carousel0"F % +4 "sampler1"I7/ +7 "core" Ơ%&JN +128 / +18 ɷ/ +3 !"preston"o:UserAbort : mesg"+Sample Pump (SP) seek to 30ml Aborted!:@timestampu: Time .Rr:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:125:in `refill'"$preston:22:in `topLevelBinding'"preston:22:in `times'"$preston:22:in `topLevelBinding'" Power.sampler :OFF +3 .Gate.power :sampler,:OFF +1975 #-> Sampler.refill +2 .Bypass.open +3 "sampler1"IE1 +153 "core" 1 +2 ."preston"Intake.open +2 "sampler0"H_1 +140 "core" 1 +2 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +5 HP% +4 "core" %B +4 ."preston"SamplePump.seek 30ml +2 "sampler0"Hm'` +1555 "Can"mbv.&ys-&? +2437 "core" ' +2 ."preston"Bypass.close +2 "sampler1"I/ +149 "core" Ɇ/ +2 ."preston"Exhaust.open +2 "sampler0"HO2 +147 "core" 2 +3 "sampler0"HD% +3 "core" %`T +3 ."preston"SamplePump.seek 55ml +2 "sampler0"Hl'-[ +3336 "core" ' +2 ."preston"delay 1 second +104 ."closeIntake"Intake.close +2 "sampler0"H/ +3 ."closeExhaust"Exhaust.close +7 "sampler0"HG0 +6 "carousel0"Fk% +73 "core" %& +57 Ȃ/ +13 0 +3 "sampler0"H% +6 "core" Ȏ%-[V +5593 "Can"lbF.,ytN*? +4960 #"preston"-> Sampler.refill +2 .Bypass.open +3 "sampler1"I"1 +152 "core" ɢ1 +2 ."preston"Intake.open +3 "sampler0"H;1 +142 "core" Ȼ1 +5 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 H1% +3 "core" ȱ% +3 ."preston"SamplePump.seek 30ml +3 "sampler0"H'` +3991 "core" ȁ' +2 ."preston"Bypass.close +3 "sampler1"Iy/ +152 "core" / +2 ."preston"Exhaust.open +2 "sampler0"H#2 +154 "core" ȣ2 +2 "sampler0"Hp% +4 "core" %`_ +3 ."preston"SamplePump.seek 55ml +2 "sampler0"Ht'-[ +2356 "Can"mb.+ys..? +556 ."preston"SamplePump.hold +9 "sampler0"Hs+ +5 "core" '@ "np 2 +6 + +3 ."preston"delay 1 second +104 ."closeIntake"Intake.close +4 "sampler0"HV/ +7 ."closeExhaust"Exhaust.close +6 "carousel0"FZ% +4 "sampler0"H90 +9 "core" %&X +124 / +26 ȹ0 +3 !"preston"o:UserAbort : mesg"+Sample Pump (SP) seek to 55ml Aborted!:@timestampu: Time .`]+:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"8/home/esp/esp2/lib/slide.rb:94:in `inConfiguration'"0/home/esp/esp2/lib/slide.rb:421:in `moveTo'"./home/esp/esp2/lib/scale.rb:198:in `pump'";/home/esp/esp2/utils/cartridge/deep.rb:128:in `refill'"$preston:25:in `topLevelBinding'" Power.sampler :ON +3 .Gate.power :sampler,:ON +564 #-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +1053 #-> Sampler.refill +3 .Bypass.open +4 "sampler1"In1 +153 "core" 1 +2 ."preston"Intake.open +2 "sampler0"Hr1 +143 "core" 1 +2 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 H|% +3 "core" % +3 ."preston"SamplePump.seek 30ml +4 "sampler0"HY'` +1055 "Can"mbJ.0ys-4@9 +2936 "core" ' +2 ."preston"Bypass.close +2 "sampler1"I / +154 "core" Ɍ/ +2 ."preston"Exhaust.open +2 "sampler0"H 2 +149 "core" ȍ2 +2 "sampler0"H\ % +3 "core" Ȋ%`b/ +4 ."preston"SamplePump.seek 55ml +3 "sampler0"Hq'-[ +3335 "core" ' +2 ."preston"delay 1 second +103 ."closeIntake"Intake.close +3 "sampler0"H+/ +5 ."closeExhaust"Exhaust.close +10 "carousel0"F2% +5 "sampler0"H0 +2 "core" Ʋ%&d +127 ȫ/ +20 Ț0 +2 "sampler0"HM% +4 "core" %-[z +5081 "Can"na.3ytN8@W +11954 map.7ytJ(;c@u +11957 na. Head.psi +2 "carousel1"G& +7 "core" Ǔ&XQ +2134 "Can"lb2. 2.times{BACsampler.prime 200,100} +2 "carousel0"F% +5 "core" Ɣ%&X +4 #"preston"Priming sample loop w/200ml, bypass w/100ml +2 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +6 ."preston"Bypass.open +2 "sampler1"I$1 +154 "core" ɤ1 +23 !"preston"o:UserAbort : mesg"User Abort!!:@timestampu: Time .Rim:@checkpoint: omitted:bt["7/home/esp/esp2/lib/cmdserver.rb:295:in `interrupt'"7/home/esp/esp2/lib/cmdserver.rb:425:in `interrupt'",preston aborter-:1:in `topLevelBinding'" Power.sampler :ON +3 .Gate.power :sampler,:ON +406 #-> 2.times{BACsampler.prime 200,100} +2 "carousel0"Fv% +6 "core" %&OrX +3 #"preston"Priming sample loop w/200ml, bypass w/100ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +77 "ambient0"JX% +4 "core" %& +2 ."preston"Intake.open +3 "sampler0"HB1 +142 "core" 1 +2 "ambient0"J&% +5 "core" ʦ%+ +3 #"preston"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H% +8 "core" %g +43 "sampler0"H8% +3 "core" ȸ%s +47 "sampler0"H?% +3 "core" ȿ%v +47 "sampler0"H% +3 "core" Ȑ%~h +47 "sampler0"H% +3 "core" ȕ%o +47 "sampler0"H(% +3 "core" Ȩ%wm +9 "Can"ka.9 e  +5 ."preston"Abs.hold +2 "ambient0"J+ +6 "core" ʛ+ +3 ."preston"PhaseSeparator.hold +2 "carousel1"G%, +7 "core" ǥ, +4 "sampler0"Hj% +3 "core" %s +3 "sampler0"H% +4 "core" Ș%qd +3 #"preston"Waiting for sampler to finish priming... + .delayUntil "10:28:35.12" +3678 "core" ǥ, +3 ."preston"SamplePump.stop +3 "sampler0"H5 +7 "carousel1"G.& +5 "core" Ǯ&}' +2 !"preston"o:)Cartridge::Sampler::LostHeadPressure: mesg""Head Pressure Fell to 0.88psi:bt["?/home/esp/esp2/utils/cartridge/sampler.rb:698:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:24:in `prime'"$preston:32:in `topLevelBinding'"preston:32:in `times'"$preston:32:in `topLevelBinding'"^ }m#(@;3 22Q +8 ."preston"SamplePump.seek 100ml,7:10 +2 "sampler0"H@'# +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@;3 22Q +7689 "Can"ka@.LysbR-SsA) +5397 "core" ' +2 "sampler0"H-% +4 "core" ȭ%#} 2 +3 ."preston"Abs.stop +2 "ambient0"J5 +6 ."preston"PhaseSeparator.stop + "carousel1"G6 +7 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."preston"Exhaust.open +2 "sampler0"HJ2 +148 "core" 2 +73 ."closeBypass"Bypass.close +4 "sampler1"I!/ +150 "core" ɡ/ +73 "sampler0"HN% +4 "core" %Z +2 "ambient0"Jc% +4 "core" % +3 ."preston"Abs.in :default +2 "ambient0"J>9_ e  +7 ."preston"Abs.hold +3 "ambient0"JQ+ +5 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"G', +7 "core" ǧ, +3 "ambient0"J% +4 "core" ʜ% +3 "sampler0"H5% +9 "core" ȵ%[ +2 "sampler0"HI% +5 "core" %U +5 ."preston"SamplePump.in :start +3 "sampler0"H>^ }m#(@V;3 22Q +7 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"H)'G +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@V;3 22Q +5933 "Can"la .UysaS]AG +11959 j`._ysZSbfAe +8311 "core" ȩ' +2 "sampler0"HR% +4 "core" %G 2 +3 ."preston"Abs.stop + "ambient0"J5 +7 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +5 "carousel0"F% +4 "core" Ɩ%&%ll +3 #"preston"Priming sample loop w/200ml, bypass w/100ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +6 ."preston"Exhaust.close +2 "sampler0"HF0 +148 "core" 0 +2 ."preston"Bypass.open +3 "sampler1"I41 +149 "core" ɴ1 +73 "ambient0"J<% +3 "core" ʼ%U +5 "Can"j`._ys:mA{ + "ambient0"J% +4 "core" ʒ%g +2 "ambient0"Jx% +4 "core" %Q +3 ."preston"Abs.in :default +2 "ambient0"J>9) e  +6 ."preston"Abs.hold +2 "ambient0"J+ +5 "core" ʞ+ +3 ."preston"PhaseSeparator.hold +2 "carousel1"Gu, +8 "core" , +2 "sampler0"HT% +5 "core" %c +3 "sampler0"HH% +4 "core" %^ +5 ."preston"SamplePump.in :start +2 "sampler0"H>^ }m#(@;3 22Q +8 ."preston"SamplePump.seek 100ml,7:10 +3 "sampler0"H3'# +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@;3 22Q +3084 "Can"k`E.fys`R\\p[A +10000 "core" ȳ' +2 "sampler0"H*% +5 "core" Ȫ%# 2 +2 ."preston"Abs.stop +2 "ambient0"J5 +5 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +5 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."preston"Exhaust.open +2 "sampler0"H/2 +149 "core" ȯ2 +74 ."closeBypass"Bypass.close +3 "sampler1"I>/ +155 "core" ɾ/ +73 "sampler0"HU% +4 "core" %v +2 "ambient0"J% +4 "core" ʙ%O +3 ."preston"Abs.in :default +3 "ambient0"J>9' e  +5 ."preston"Abs.hold +2 "ambient0"Jg+ +7 "core" + +2 ."preston"PhaseSeparator.hold +2 "carousel1"Gh, +6 "core" , +57 "ambient0"J% +4 "core" ʑ%R +5 "sampler0"Hi% +4 "core" %o +3 "sampler0"He% +4 "core" %m +8 ."preston"SamplePump.in :start +3 "sampler0"H>^ }m#(@V;3 22Q +8 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"H='G +55 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@V;3 22Q +1273 "Can"m`.gysZRyA +11952 k_.pys^RA +11953 l`.vysIXUA +1013 "core" Ƚ' +2 "sampler0"H^% +4 "core" %G 2 +3 ."preston"Abs.stop +2 "ambient0"J5 +10 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +3738 #"preston"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +2896 #-> Power.sampler :ON +3 .Gate.power :sampler,:ON +4278 "Can"m`.sys?(;A +299 #"preston"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +11652 "Can"na0.nytMB +9114 #"preston"-> SC.coast +2 .Carousel.coast +2 "carousel0"F5 +6 FC% +3 "core" %&ns +2829 "Can"ma.nytNB7 +11956 l`.lytNgBBU +11953 l`.pytMBs +11955 l`!.oytPB +11952 o`.pytN0B +11955 l`X.pytKB +11958 l` .uytM}B +11956 l_.yytI%C +11955 q_p.}ytMC' +11953 l_.}ytNxCE +1189 #"preston"-> BACsampler.prime +4 "carousel0"F % +3 "core" Ƌ%&X- E +4 #"preston"Priming sample loop w/200ml, bypass w/10ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +6 ."preston"Exhaust.close +2 "sampler0"Hd0 +151 "core" 0 +3 ."preston"Bypass.open +3 "sampler1"Ib1 +151 "core" 1 +73 "ambient0"J{% +4 "core" %! +5 "Can"l_.}yt4'CI + "ambient0"J[% +4 "core" %! +3 "ambient0"J6% +4 "core" ʶ%j +3 ."preston"Abs.in :default +2 "ambient0"J>9 e  +6 ."preston"Abs.hold +2 "ambient0"Jw+ +5 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"G\\, +7 "core" , +3 "sampler0"Hz% +5 "core" % +3 "sampler0"HK% +5 "core" % +3 #"preston"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +9 "core" ,A +2 LJ&P +3 ."preston"SamplePump.stop +3 "sampler0"H5 +5 "carousel1"Go& +5 "core" &e +3 !"preston"o:)Cartridge::Sampler::LostHeadPressure: mesg""Head Pressure Fell to 0.03psi:bt["?/home/esp/esp2/utils/cartridge/sampler.rb:698:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:24:in `prime'"$preston:37:in `topLevelBinding'"^ }m#(@;3 22Q +11 ."preston"SamplePump.seek 10ml,1:10 +2 "sampler0"H`'6 +54 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@;3 22Q +1286 "core" ' +2 "sampler0"H7% +4 "core" ȷ%6N 2 +3 ."preston"Abs.stop +2 "ambient0"J5 +5 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +6 ."preston"SamplePump.setPosition! 0ml + "sampler0"H7 +6 ."preston"Exhaust.open +2 "sampler0"Hf2 +150 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"IE/ +149 "core" / +73 "sampler0"H_% +3 "core" % +3 "ambient0"JP% +4 "core" % +3 ."preston"Abs.in :default +2 "ambient0"J>9 e  +6 ."preston"Abs.hold +3 "ambient0"J:+ +5 "core" ʺ+ +3 ."preston"PhaseSeparator.hold +2 "carousel1"G, +6 "core" dž, +3 "ambient0"JO% +4 "core" % +3 "sampler0"HD% +3 "core" % +3 "sampler0"Hm% +5 "core" %" +6 ."preston"SamplePump.in :start +2 "sampler0"H>^ }m#(@V;3 22Q +7 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"Hk'G +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@V;3 22Q +2241 "Can"k_.ys`RCc +11954 l_.ys^S/ˆC +11957 k_.ysZSMC +43 "core" ' +2 "sampler0"H% +3 "core" Ȃ%G\\P 2 +62 ."preston"Abs.stop +5 "ambient0"J5 +11 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +10162 #"preston"-> BACsampler.prime +3 "carousel0"FG% +5 "core" %&ԩmiw +3 #"preston"Priming sample loop w/200ml, bypass w/10ml +3 .SamplePump.setPosition! 0ml +3 "sampler0"H7 +5 ."preston"Exhaust.close +3 "sampler0"H0 +151 "core" Ȏ0 +2 ."preston"Bypass.open +2 "sampler1"I"1 +152 "core" ɢ1 +73 "ambient0"J;% +3 "core" ʻ%w +6 "Can"k_.ys;$C + "ambient0"J1% +4 "core" ʱ%u +2 "ambient0"Jl% +5 "core" %J +3 ."preston"Abs.in :default +2 "ambient0"J>9 e  +6 ."preston"Abs.hold +2 "ambient0"Jy+ +5 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"G#, +7 "core" ǣ, +3 "sampler0"Hp% +4 "core" %% +4 "sampler0"H% +3 "core" ȁ%# +5 ."preston"SamplePump.in :start +2 "sampler0"H>^ }m#(@;3 22Q +9 ."preston"SamplePump.seek 10ml,1:10 +3 "sampler0"HZ'6 +56 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@;3 22Q +1122 "Can"l^.ys\\SӀC +162 "core" ' +3 "sampler0"HV% +5 "core" %6jS 2 +2 ."preston"Abs.stop +2 "ambient0"J5 +5 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +5 ."preston"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."preston"Exhaust.open +2 "sampler0"Ht2 +152 "core" 2 +74 ."closeBypass"Bypass.close +3 "sampler1"I9/ +154 "core" ɹ/ +73 "sampler0"Hn% +4 "core" %x +2 "ambient0"Jr% +4 "core" %B +3 ."preston"Abs.in :default +2 "ambient0"J>9 e  +5 ."preston"Abs.hold +2 "ambient0"J|+ +6 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"Gs, +6 "core" , +2 "ambient0"J % +5 "core" ʌ% +4 "sampler0"H % +4 "core" ȍ% +3 "sampler0"H\ % +4 "core" Ȋ% +5 ."preston"SamplePump.in :start +4 "sampler0"H>^ }m#(@V;3 22Q +9 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"HY'G +53 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@V;3 22Q +6720 ."preston"SamplePump.hold +7 "sampler0"H2+ +13 "core" ' .U1 2 +2 Ȳ+ +4 "sampler0"H+% +5 "core" ȫ%R 2 +3 ."closeIntake"Intake.close +4 "sampler0"H/ +7 ."closeExhaust"Exhaust.close +9 "carousel0"FM% +4 "sampler0"H0 +11 "core" %&Ԩ{g +117 Ț/ +24 ȓ0 +4 ."preston"Abs.stop +4 "ambient0"J5 +6 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +9 !"preston"o:UserAbort : mesg",Sample Pump (SP) seek to 200ml Aborted!:@timestampu: Time /?:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"@/home/esp/esp2/utils/cartridge/sampler.rb:624:in `pumpSeek'"=/home/esp/esp2/utils/cartridge/sampler.rb:509:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'"$preston:38:in `topLevelBinding'" sampler :OFF +4 !o:NoMethodError\ : mesg";undefined method `sampler' for CmdServer::Client:Class:@timestampu: Time /7?: name0: args0:bt[";/home/esp/esp2/lib/framework.rb:51:in `method_missing'";/home/esp/esp2/lib/framework.rb:63:in `method_missing'"$preston:39:in `topLevelBinding'" Power.sampler :OFF +3 .Gate.power :sampler,:OFF +1696 #-> Power.sampler :ON +2 .Gate.power :sampler,:ON +1339 #-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +168 "Can"m].ytNۀC +1566 #"preston"-> to 50 +3 "valve0"D% +5 "core" Ĕ%8['Oy +3 "syringe1"C$& +3 "core" ä&lZ +3 "syringe1"Cv% +4 "core" %ݨ;a +3 "valve1"EX& +4 "core" &YOw  +4 ."preston"Carousel.seek 50 +2 "carousel0"FB'/ +31 "core" ' +4 "carousel0"F&% +3 "core" Ʀ%//V 2 +3 ."preston"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C& +3 "core" &l~ +4 ."preston"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."preston"Lysis1.seek :locked +4 "core" ' /UV!W  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 50 + 5 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi:@pastThresholdF: @voltagef14.886631320569466 : @currentiZ: @type2F:@outPressiW!:\ @lostF: @inPressiV:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni/: @pastRLSF: @tagiG: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +2 ' + "syringe1"C8( +249 "core" ø( +3 "valve0"D?% +7 "core" Ŀ%8[O +3 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +4 "syringe1"C% +2 "valve0"D'Z +7 "core" Ð%ݥ~ +7 ."Air"Air.in :fast +3 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"C('6 +72 "core" ĕ' +32 è' +3 ."preston"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F~% +3 "core" %/b?B  +8 #"preston"Powering cartridge bus +264 "cartridge_broadcast" ( +6 ."preston"Gate.power :cartridge,:OFF +6 #Mounted archive Cartridge +3411 #-> delay 10;TV.cartridge;delay 5;TV.bypass +2 .delay 10 seconds +1005 "carousel0"F% +3 "core" Ƅ%/?np \ +6 ."preston"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."preston"Toroid.seek :cartridge +2 "valve0"Dj(m +373 "core" ( +5 ."preston"Toroid.in :default +2 "valve0"D? ֜2 +6 ."preston"Toroid.seek :clear +3 "valve0"D( +373 "core" Ę( +2 ."preston"delay 5 seconds +503 "carousel0"F.% +3 "core" Ʈ%/> \ +6 ."preston"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."preston"Toroid.seek :bypass +2 "valve0"Dq( +355 "core" ( +5 ."preston"Toroid.in :default +2 "valve0"D? ֜2 +6 ."preston"Toroid.seek :clear +3 "valve0"D%( +354 "core" ĥ( +2 "valve0"D & +4 "core" ĉ& R_  +979 #"preston"-> ESP.reready! +6 ."Plunge_home"Plunge.in :default +6 ."Toroid_home"Toroid.in :default +4 "valve0"D? ֜2 +11 D> P ޤ-2 +11 ."Carousel_home"Carousel.in :default +6 ."Archive_home"Archive.in :default +5 ."Toroid_home"Toroid.home.jog 0 +4 ."Air_home"Air.in :default +5 ."Lysis1_home"Lysis1.in :default +4 ."Lysis2_home"Lysis2.in :default +6 ."Plunge_home"Plunge.home.jog 0 +4 ."PinPort_home"PinPort.in :default +6 "carousel0"F><` F 22 +4 "syringe0"B?d PZ<2 +2 "valve0"D0* + "syringe1"C>d P]z<2 +4 C?d P[<2 +2 "syringe0"B>d P\\k<2 +2 "valve0"D) + "valve1"E? B22 +7 ."Carousel_home"Carousel.home.jog 0 +10 "carousel0"F) +13 "core" İ* +5 ."PinPort_home"PinPort.home.jog 0 +11 ."Lysis1_home"Lysis1.home.jog 0 +4 "core" ě) +2 ."Air_home"Air.home.jog 0 +13 ."Archive_home"Archive.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +4 "valve1"E-* +3 "valve0"DJ& +6 "syringe1"C,* +2 "valve0"Da% + "syringe1"C!) +5 "syringe0"BN* +15 Bc) +13 "core" ƃ) +2 ŭ* + &Od  + %dG}OM  + ì* + á) + * +3 "syringe1"C& +10 C5% +5 ."Plunge_home"Plunge.in :homing +9 "valve1"EI& + "syringe0"B@& +11 ."Toroid_home"Toroid.in :homing +6 "valve0"D> (2 +3 D?2 +5 ."Plunge_home"Plunge.jog 1462 +3 ."Toroid_home"Toroid.jog 1360 +5 "valve0"DR) +3 D*P +9 "core" ) + Ü&"s  + õ%';]  + &GOe + &w/ss +2 "syringe0"BF% +13 ."Air_home"Air.in :homing +3 ."PinPort_home"PinPort.in :homing +4 ."Lysis1_home"Lysis1.in :homing +5 ."Archive_home"Archive.in :homing +4 "syringe1"C> -2 +2 "valve1"E?2 +6 "syringe1"C? (2 +3 "syringe0"B? -2 +3 ."PinPort_home"PinPort.jog 1225 +7 ."Air_home"Air.jog 1233 +10 ."Lysis1_home"Lysis1.jog 2265 +6 ."Archive_home"Archive.jog 1615 +4 "valve1"EQ* +5 "syringe1"C') +4 C4* + "syringe0"B<*O +16 "core" %wٯrT  +3 ."Lysis2_home"Lysis2.in :homing +2 "syringe0"B> (2 +6 ."Lysis2_home"Lysis2.jog 1616 +3 "syringe0"B)P +7 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"Dx' +25 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +27 "core" ç) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +5 ."Air_home"Air.seek :home +3 "syringe1"CT' +8 "core" ' + ¼* +4 ."Archive_home"Archive.setRawPosition! 50 +3 ."Air_home"Air.in :slow +3 "syringe0"B82 +3 "syringe1"C>d P]z<2 +8 ."Archive_home"Archive.seek :home +7 "syringe0"BH( +7 "core" ’) + ô* + * +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +5 ."PinPort_home"PinPort.setRawPosition! 3150 +5 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 "syringe0"B72 +2 "valve1"E8 N +4 "syringe1"C82 +59 ."Lysis2_home"Lysis2.seek :home +15 ."PinPort_home"PinPort.seek :home +5 "syringe0"B)' +3 ."Lysis1_home"Lysis1.seek :home +7 "valve1"E*( +4 "syringe1"C/( +10 "core" ( +3 ."Archive_home"Archive.in :default +3 "syringe0"B?d PZ<2 +8 "core" ©' +3 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +4 "core" ï( +4 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +33 "core" Ė* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +8 "valve0"D( +6 "core" Ğ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +334 "core" Ū( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +36 ."clearToroid"Toroid.seek :clear +3 "valve1"Eu& +3 "valve0"D>( +9 "core" &O[  +6 ."preston"PinPort.in :unlocking +3 "valve1"E? B2 +6 ."preston"PinPort.seek :clear +2 "valve1"EU( +43 "core" ( +286 ľ( +3 ."preston"Air.seek :clear +2 "syringe1"C' +8 "core" Ù' +2 ."preston"Lysis1.seek :clear +3 "syringe1"C( +69 "core" Ñ( +3 ."preston"Lysis2.seek :clear +4 "syringe0"Bi'j +74 "core" ' +2 ."preston"Archive.seek :clear +3 "syringe0"Be(j +76 "core" ( +6 ."preston"Carousel.in :default +2 "carousel0"F><` F 22 +6 F3% +3 "core" Ƴ%/d?  +4 ."preston"Carousel.home.seek 50 +3 "carousel0"F^'/ +17 "core" ' +3 "valve0"Dg% +3 "core" %^OS !r +3 "valve0"Dh% +4 "core" %^OE !| +3 "sampler0"HC% +5 "core" %E +3 "valve0"D & +5 "core" ċ&ON  +3 "carousel0"Fd% +5 "core" %/`?w  +2 "ambient0"Jb% +3 "core" % +3 "carousel1"G{& +4 "core" &`l +3 "syringe0"B[& +4 "core" &jG +3 "syringe1"C6% +5 "core" ö%ݶdi +2 "syringe1"Cz& +5 "core" &وc +2 "syringe0"BK% +4 "core" %jٸ_y +3 "valve1"Eo& +4 "core" &yOO  +272 "Can"k^.yt<C +1737 #"preston"-> BACsampler.prime 200,nil +2 "carousel0"F=% +5 "core" ƽ%/_?  +3 #"preston"Priming sample loop w/200ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +5 ."preston"Bypass.open +2 "sampler1"I\\1 +149 "core" 1 +73 "ambient0"J% +4 "core" ʇ%N +5 "Can"k^.yt%~ߚC + "ambient0"JS% +4 "core" %M +3 ."preston"Exhaust.open +2 "sampler0"H 2 +154 "core" Ƞ2 +73 ."closeBypass"Bypass.close +5 ."preston"Intake.open +6 "sampler1"Iw/ +2 "sampler0"HA1 +142 "core" 1 +4 / +72 "sampler0"H7% +4 "core" ȷ%* +3 "ambient0"Jf% +3 "core" %? +3 ."preston"Abs.in :default +2 "ambient0"J>9} e  +6 ."preston"Abs.hold +2 "ambient0"J]+ +5 "core" + +3 ."preston"PhaseSeparator.hold +2 "carousel1"G, +7 "core" Dž, +2 "ambient0"JE% +4 "core" %? +3 "sampler0"H_% +5 "core" %% +3 "sampler0"HP% +6 "core" %) + Dž,`9 +3 ."preston"SamplePump.stop +2 "sampler0"H5 +7 "carousel1"GO& +3 "core" &ad +3 !"preston"o:)Cartridge::Sampler::LostHeadPressure: mesg""Head Pressure Fell to 0.02psi:bt["?/home/esp/esp2/utils/cartridge/sampler.rb:698:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'"$preston:46:in `topLevelBinding'"_DK +5 ."preston"SamplePump.in :start +2 "sampler0"H>^ }m#(@V;3 22Q +7 ."preston"SamplePump.seek 200ml,13:50 +3 "sampler0"HG'G +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(@V;3 22Q +3306 "Can"j^.ysHWcD +11956 i^2.ysPW D5 +7885 ."preston"SamplePump.hold +12 "sampler0"H+ +5 "core" 'ɀ 3g 2 +4 Ȏ+ +4 "sampler0"H"% +4 "core" Ȣ%ɋgq 2 +4 ."closeIntake"Intake.close +6 ."closeExhaust"Exhaust.close +7 "sampler0"H;/ +3 "carousel0"F1% +6 "sampler0"Hl0 +11 "core" Ʊ%/a@{b  +130 Ȼ/ +13 0 +4 ."preston"Abs.stop +2 "ambient0"J5 +9 ."preston"PhaseSeparator.stop +2 "carousel1"G6 +8 !"preston"o:UserAbort : mesg",Sample Pump (SP) seek to 200ml Aborted!:@timestampu: Time /i#_:@checkpoint: omitted:bt["7/home/esp/esp2/lib/slide.rb:390:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"6/home/esp/esp2/lib/scale.rb:93:in `unlimitedSeek'",/home/esp/esp2/lib/pump.rb:34:in `seek'"@/home/esp/esp2/utils/cartridge/sampler.rb:624:in `pumpSeek'"=/home/esp/esp2/utils/cartridge/sampler.rb:509:in `pump!'"+/home/esp/esp2/lib/slide.rb:94:in `in'"=/home/esp/esp2/utils/cartridge/sampler.rb:495:in `pump!'"?/home/esp/esp2/utils/cartridge/sampler.rb:663:in `sample!'"=/home/esp/esp2/utils/cartridge/sampler.rb:617:in `servo'"?/home/esp/esp2/utils/cartridge/sampler.rb:661:in `sample!'",/home/esp/esp2/lib/msg.rb:68:in `retry'"?/home/esp/esp2/utils/cartridge/sampler.rb:652:in `sample!'"9/home/esp/esp2/utils/cartridge/deep.rb:36:in `prime'"$preston:46:in `topLevelBinding'" ampler :OFF +3 !o:NoMethodError\ : mesg":undefined method `ampler' for CmdServer::Client:Class:@timestampu: Time /D4_: name0: args0:bt[";/home/esp/esp2/lib/framework.rb:51:in `method_missing'";/home/esp/esp2/lib/framework.rb:63:in `method_missing'"$preston:47:in `topLevelBinding'" Power.sampler :OFF +2 .Gate.power :sampler,:OFF +2986 "Can"l^.yt:!DS +11958 n^.yt?Dq +11959 m^1.yt:*D +4061 #"preston"-> SC.to 22 +5 .Carousel.seek 22 +2 "carousel0"Fp' +594 "core" ' +2 "carousel0"F% +4 "core" Ɓ%6[ 2 +7163 #"preston"-> slots +125 "Can"l^.yt<]D +2626 #"preston"-> slot 22=>[:dry,:microSPR_phyto] +4544 #-> MainThread.exit +4 "sampler0"Hk% +4 "core" %ɋ +7 #mEXIT +5 !"preston"o:ThreadError: mesg"killed thread:@timestampu: Time /v!~:bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6 #Session Ended @1785598299.67EDT @1785598308.88EDT = =mMAIN_scheduler =tMAIN @EDT1785598310.72 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +141 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +30 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +29 "Can"k].yt9D +328 #t\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +71 #Sea pressure (Abs) tare is 6% of full scale +375 "Can"k].yt(D + k].yt(D + #tPolling Can every 2:00 +4 #Waiting for espclients... +48 "Can"k].yt>|D4 +142 #"nadia"Session Begun +811 #-> ESP.ready! +19 .Gate.power :sampler,:OFF,limit to 3500mA + .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +53 "valve0"DP +3 D +3 "core" ā +6 "valve0"D% +3 "core" Ă%^O_ !g +3 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +11 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +4 "sampler0"H% +4 "core" ȃ%ɋs +2 ."nadia"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 "valve0"D& +3 "core" Ą&OX  +3 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +7 ."nadia"Toroid.hold +2 "valve0"D, +4 "core" ą, +3 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +8 "carousel0"F% +3 "core" Ɔ%W[  +3 ."nadia"Carousel.configure :default +2 "carousel0"F#<` F 22 +10 ."nadia"Carousel.hold +2 "carousel0"F+ +5 "core" Ƈ+ +3 ."nadia"Carousel.configure :default + "carousel0"F#<` F 22 +9 "ambient0"J% +4 "core" ʈ% +2 ."nadia"Abs.configure :default +3 "ambient0"J#  +8 "carousel1"G & +3 "core" lj&V: +3 ."nadia"PhaseSeparator.configure :default +2 "carousel1"G$ +9 "syringe0"B\ & +3 "core" Š&j5Ri +3 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +6 ."nadia"Archive.hold +2 "syringe0"B , +5 "core" ‹, +3 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +11 "syringe1"C % +3 "core" Ì%ݴȀ[ +3 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."nadia"Air.hold + "syringe1"C + +7 "core" Í+ +2 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 C& +4 "core" Î&كR +3 ."nadia"Lysis1.configure :default + "syringe1"C$d P[<2 +7 ."nadia"Lysis1.hold +2 "syringe1"C, +6 "core" Ï, +3 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 "syringe0"B% +3 "core" %jٶJ +2 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +6 ."nadia"Lysis2.hold +3 "syringe0"B+ +4 "core" ‘+ +3 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 "valve1"E& +6 "core" Œ&rO]  +3 ."nadia"PinPort.configure :default + "valve1"E$ B22 +7 ."nadia"PinPort.hold +2 "valve1"E, +5 "core" œ, +3 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +6 #"nadia"All elf controllers running firmware version 4.94 +7 "valve0"D% +5 "core" Ĕ%^O_  +2 "valve0"D& +4 "carousel0"F% +6 "syringe0"B& +5 "syringe1"C% +6 C& +7 "syringe0"B% +6 "valve1"E& +23 "core" ĕ&O_ \ + Ɩ%W[  + —&j>NX Ø%ݸB + Ù&yZ + š%j٦MT + ś&rO^  +8 ."clearToroid"Toroid.seek :clear +4 "valve0"D( +3 "valve1"E& +9 "core" ŝ&jO^  +2 Ĝ( +4 ."nadia"Air.seek :clear +3 "syringe1"C' +5 "core" Þ' +3 ."nadia"Lysis1.seek :clear +3 "syringe1"C( +5 "core" ß( +4 ."nadia"Lysis2.seek :clear +2 "syringe0"B 'j +49 "core"  ' +2 ."nadia"Archive.seek :clear +3 "syringe0"B!(j +6 "core" ¡( +4 ."nadia"Carousel.in :default + "carousel0"F><` F 22 +6 F"% +3 "core" Ƣ%V[xt  +3 ."nadia"Carousel.home.seek 22 +3 "carousel0"F#' +27 "core" ƣ' +2 "valve0"D$% +5 "core" Ĥ%^OX  +4 "valve0"D%% +4 "core" ĥ%^OS  +3 "sampler0"H&% +6 "core" Ȧ%ɋ} +2 "valve0"D'& +5 "core" ħ&OU  +3 "carousel0"F(% +4 "core" ƨ%`[9  +3 "ambient0"J)% +4 "core" ʩ% +3 "carousel1"G*& +5 "core" Ǫ&`| +3 "syringe0"B+& +3 "core" «&j5K +3 "syringe1"C,% +4 "core" ì%ݵM +3 "syringe1"C-& +5 "core" í&نJ +2 "syringe0"B.% +4 "core" ®%j٥K +3 "valve1"E/& +3 "core" ů&wOR  +233 #"nadia"-> slots +517 #-> ESP.reready! +4 ."Plunge_home"Plunge.in :default +5 "valve0"D> P ޤ-2 +5 ."Toroid_home"Toroid.in :default +10 ."Carousel_home"Carousel.in :default +4 ."Plunge_home"Plunge.home.jog 0 +7 "valve0"D? ֜2 +4 ."Archive_home"Archive.in :default +10 "carousel0"F><` F 22 +5 ."Air_home"Air.in :default +5 "valve0"D0) +6 ."Lysis1_home"Lysis1.in :default +3 ."Lysis2_home"Lysis2.in :default +8 "syringe0"B?d PZ<2 +3 ."PinPort_home"PinPort.in :default +5 ."Toroid_home"Toroid.home.jog 0 +4 "syringe1"C>d P]z<2 +5 ."Carousel_home"Carousel.home.jog 0 +8 "syringe1"C?d P[<2 +5 "syringe0"B>d P\\k<2 +3 "core" İ) +2 ."Archive_home"Archive.home.jog 0 +6 "valve1"E? B22 +5 "valve0"D1* +6 "carousel0"F2) +2 ."Air_home"Air.home.jog 0 +5 "valve0"D3% +4 "syringe0"B4* +3 ."Lysis1_home"Lysis1.home.jog 0 +7 ."Lysis2_home"Lysis2.home.jog 0 +8 "syringe1"C5) +4 C6* +3 "syringe0"B7) +2 ."PinPort_home"PinPort.home.jog 0 +6 "valve1"E8* +33 "core" ı* +2 ij%^OV Ʋ) + ´* + õ) ö* + ·) + Ÿ* +2 "syringe0"B9% +11 "syringe1"C:& +5 "valve1"E;& +4 "syringe1"C<% +2 "valve0"D=& +4 "syringe0"B>& +3 ."Plunge_home"Plunge.in :homing +18 "valve0"D> (2 +6 "core" ¹%vټw + ú&|y  + Ż& oO` + ü%\ ݵu Ľ&Oa + ¾&z4;  +7 ."Plunge_home"Plunge.jog 419 +9 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +8 ."Toroid_home"Toroid.in :homing +3 ."PinPort_home"PinPort.in :homing +4 ."Archive_home"Archive.in :homing +4 ."Lysis1_home"Lysis1.in :homing +5 "valve0"D?) +4 "syringe0"B> (2 + "syringe1"C> -2 +8 "valve0"D?2 +2 "valve1"E?2 +4 "syringe0"B? -2 +10 "syringe1"C? (2 +4 ."Lysis2_home"Lysis2.jog 1615 +7 ."PinPort_home"PinPort.jog 1221 +5 ."Air_home"Air.jog 1424 +4 ."Toroid_home"Toroid.jog 1364 +4 ."Lysis1_home"Lysis1.jog 1595 +6 ."Archive_home"Archive.jog 1614 +4 "syringe0"B@)O +3 "valve1"EA* +8 "syringe1"CB) +2 "valve0"DC*T +9 "syringe0"BE*N + "syringe1"CD*; +17 "core" Ŀ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +5 ."Plunge_home"Plunge.seek :home +3 "valve0"DF' +25 "core" ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +47 "core" ) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"BG' +7 "core" ) +2 ."Air_home"Air.setRawPosition! 0 +3 "syringe1"C7 +5 ."Air_home"Air.seek :home +3 "syringe1"CH' +8 "core" * + * ' + ' +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +4 ."Air_home"Air.in :default +5 ."Lysis2_home"Lysis2.in :default +4 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe1"C82 +2 C>d P]z<2 +5 "syringe0"B>d P\\k<2 +4 B82 +3 ."Lysis1_home"Lysis1.seek :home +5 "syringe1"CI( +3 ."Archive_home"Archive.seek :home +10 "syringe0"BJ( +4 "core" * +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 "valve1"E8 N +5 ."PinPort_home"PinPort.seek :home +4 "valve1"EK( +9 "core" ( + ( +3 ."Archive_home"Archive.in :default +3 ."Lysis1_home"Lysis1.in :default +3 "syringe0"B?d PZ<2 +4 "syringe1"C?d P[<2 +212 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"DL( +5 "core" ( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +178 "core" ( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +8 "valve0"DN( +3 "valve1"EM& +56 "core" &Q +4 ."nadia"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."nadia"PinPort.seek :clear +2 "valve1"EO( +43 "core" ( +289 ( +2 ."nadia"Air.seek :clear +3 "syringe1"CP' +8 "core" ' +3 ."nadia"Lysis1.seek :clear +2 "syringe1"CQ( +70 "core" ( +3 ."nadia"Lysis2.seek :clear +3 "syringe0"BR'j +76 "core" ' +3 ."nadia"Archive.seek :clear +3 "syringe0"BS(j +75 "core" ( +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 FT% +3 "core" %c[  +3 ."nadia"Carousel.home.seek 22 +3 "carousel0"FU' +17 "core" ' +3 "valve0"DV% +4 "core" %^Os !X +3 "valve0"DW% +3 "core" %^On !> +4 "sampler0"HX% +5 "core" %ɋj +2 "valve0"DY& +5 "core" &bOr P +3 "carousel0"FZ% +4 "core" %`[[  +3 "ambient0"J[% +4 "core" % +3 "carousel1"G\\& +4 "core" &au +3 "syringe0"B]& +5 "core" &j7jx +3 "syringe1"C^% +4 "core" %ݶd +3 "syringe1"C_& +3 "core" &ٌnh +3 "syringe0"B`% +4 "core" %j j +3 "valve1"Ea& +4 "core" & Oi g +7649 "Can"l].yt8[D4 +8402 #"nadia"-> lysis +3557 "Can"k]}.yt< E4 +6173 #"nadia"-> Power.sampler :ON +2 .Gate.power :sampler,:ON +3681 #-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +2100 "Can"l]9.yt;*E4 +7502 #"nadia"-> start {fire lysis} +20 "valve0"Db% +3 Dc& +6 "core" %^OR !O +2 "carousel0"Fd% +7 "syringe0"Be& +8 "syringe1"Cf% +4 Cg& +4 "syringe0"Bh% +3 "valve1"Ei& +22 "core" &bOY P + %`[  &j=Ku + %ݿKg + &يU + %jٿ- + &OU \\ +8 ."clearToroid"Toroid.seek :clear +4 "valve1"Ej& +2 "valve0"Dk( +9 "core" &OV l + ( +4 ."detached from nadia"Air.seek :clear +3 "syringe1"Cl' +5 "core" ' +3 ."detached from nadia"Lysis1.seek :clear +2 "syringe1"Cm( +7 "core" ( +3 ."detached from nadia"Lysis2.seek :clear +3 "syringe0"Bn'j +5 "core" ' +4 ."detached from nadia"Archive.seek :clear +5 "syringe0"Bo(j +6 "core" ( +3 ."detached from nadia"Carousel.in :default + "carousel0"F><` F 22 +7 Fp% +3 "core" %`[x  +4 ."detached from nadia"Carousel.home.seek 22 +3 "carousel0"Fq' +17 "core" ' +3 "valve0"Dr% +4 "core" %^OU !D +4 #"detached from nadia"Selecting Cartridge 22 +2 "valve0"Ds% +8 "core" %^OZ !8 +2 "syringe1"Ct& +5 "core" &ىH{ +3 "syringe1"Cu% +4 "core" %ݮFt +3 "valve1"Ev& +4 "core" &OY g +3 ."detached from nadia"Carousel.seek 22 +2 "carousel0"Fw' +17 "core" ' +5 "carousel0"Fx% +4 "core" %a[_  +3 ."detached from nadia"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"Cy& +3 "core" &وHq +4 ."detached from nadia"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from nadia"Lysis1.seek :locked +2 "syringe1"Cz( +251 "core" ( +5 "valve0"D{% +7 "core" %^Ob !N +3 ."Plunge"Plunge.seek :locked +3 "valve0"D|'Z +8 "syringe1"C}% +6 "core" %ݹk +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"C~'6 +79 "core" ' +25 ' +2 ."detached from nadia"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F% +3 "core" %d[o  +6 #"detached from nadia"Powering cartridge bus +266 "cartridge_broadcast" ( +3 ."detached from nadia"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from nadia"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from nadia"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" t +5 "cartridge" t +3 "Cartridge" y +4 "cartridge" y&:&: +43 "Cartridge" y  +4 "cartridge" y @?+0J59951 A+0C@ +4 #"detached from nadia"Mounted lysis Cartridge board#J59951 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"D% +3 "core" Ą%ZGVOi  +3 "syringe1"C& +4 "core" Æ&/v +2 "syringe1"C% +4 "core" È%6-y % +3 "carousel0"F % +4 "core" Ɖ%[~ \ +12 #"FILTERING"Preparing to filter microSPR_phyto cartridge in slot 22 +4 "valve0"D\ % +7 "core" Ċ%ZGWOd  +6 "syringe1"C & +5 "core" Ì&2u +2 "syringe1"C% +4 "core" Î%64n  +4 #"FILTERING"Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi +2 "carousel1"G& +8 "core" ǐ&hu +42 "carousel1"G& +3 "core" ǒ&m +47 "carousel1"G& +4 "core" ǔ&w +46 "carousel1"G& +4 "core" Ǜ& +46 "carousel1"G& +4 "core" Ǚ&d +46 "carousel1"G& +3 "core" ǘ& +47 "carousel1"G& +3 "core" ǚ& +47 "carousel1"G& +3 "core" Ǘ&~ +48 "carousel1"G& +3 "core" ǖ& +47 "carousel1"G& +4 "core" Ǖ&{ +46 "carousel1"G& +4 "core" ǝ&| +46 "carousel1"G"& +4 "core" Ǣ& +46 "carousel1"G$& +3 "core" Ǥ&W +47 "carousel1"G%& +3 "core" ǥ& +5 "Can"l]9.yt0E6 + #"FILTERING"Intake Head Pressure tare is 1% of full scale at 28C (drifted 6%) +2 #Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi +5 .PhaseSeparator.in :filling +2 "carousel1"G?"d"d +6 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G&, +5 "core" Ǧ, +3 ."FILTERING"Gate.power :sampler,:ON +739 "core" Ǧ,u, +6 #"FILTERING"Head pressure reached 1.70psi in 8 seconds +4 ."sealing"Air.in :default +5 "carousel0"F'% +7 "syringe1"C>d P]z<2 +3 "core" Ƨ%[-@ \ +3 ."sealing"Air.seek :sealed +4 #"FILTERING"Priming sample loop w/200ml, bypass w/50ml +5 "syringe1"C(' +3 ."FILTERING"SamplePump.setPosition! 0ml +4 "sampler0"H7 +5 ."FILTERING"Exhaust.close +3 "sampler0"H)0 +8 "core" ȩ0 +2 ."FILTERING"Bypass.open +3 "sampler1"I*1 +153 "core" ɪ1 +72 "ambient0"J+% +3 "core" ʫ% u +3 ."FILTERING"Intake.open +2 "sampler0"H,1 +11 "core" è' +136 Ȭ1 +2 "ambient0"J-% +4 "core" ʭ% +5 #"FILTERING"Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi +2 "sampler0"H.% +6 "core" Ȯ%r1 +44 "sampler0"H/% +4 "core" ȯ%h6 +48 "sampler0"H% +3 "core" ȅ%f. +45 "sampler0"H % +4 "core" ȍ%b +46 "sampler0"H% +3 "core" ȏ%] +47 "sampler0"H% +3 "core" ȑ% +6 "Can"l]9.ysBrE:P + #"FILTERING"Vacuum tare is 54% of full scale at 28C (drifted 2%) +2 "ambient0"J % +6 "core" ʋ% +2 "ambient0"J% +4 "core" ʇ%} +2 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"J3+ +5 "core" ʳ+ +3 ."FILTERING"PhaseSeparator.in :full +2 "carousel1"G? +10 ."FILTERING"PhaseSeparator.hold +3 "carousel1"G9, +6 "core" ǹ, +3 "sampler0"H:% +4 "core" Ⱥ% +3 "sampler0"H<% +4 "core" ȼ% +3 #"FILTERING"Waiting for sampler to finish priming... +2 .delayUntil "11:40:33.38" +1075 "Can"j].ysAE=4 +3399 j].ysBUEE +4 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(B@3 22Q +8 ."FILTERING"SamplePump.seek 50ml,3:50 +2 "sampler0"H>' +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(B@3 22Q +6529 "core" Ⱦ' +2 "sampler0"H=% +4 "core" Ƚ% 2 +2 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +5 ."FILTERING"SamplePump.setPosition! 0ml +2 "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"H;2 +150 "core" Ȼ2 +73 ."closeBypass"Bypass.close +4 "sampler1"I/ +154 "core" ɜ/ +74 "sampler0"H#% +4 "core" ȣ% +2 "ambient0"J!% +4 "core" ʡ% +3 ."FILTERING"Abs.in :default +3 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold + "ambient0"J+ +6 "core" ʞ+ +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"G, +6 "core" ǟ, +2 "ambient0"J % +46 "core" ʠ% +4 "sampler0"H% +4 "core" ȓ% +2 "sampler0"H0% +4 "core" Ȱ%\ +14 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ }m#(B@3 22Q +8 ."FILTERING"SamplePump.seek 200ml,13:50 +2 "sampler0"H7'G +57 ."pumpStarted"SamplePump.configure :prime +2 "sampler0"H#d }m#(B@3 22Q +1282 "Can"n]Q.ys9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"JT+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold +2 "carousel1"GV, +8 "core" , +2 "sampler0"HW% +4 "core" %] +3 "sampler0"HX% +4 "core" % +3 ."FILTERING"SamplePump.in :charge +2 "sampler0"H>mu0r3 22Q +8 ."FILTERING"SamplePump.seek 2.3ml,38 seconds +3 "sampler0"HH' +1253 "core" 'x 2 +5 "sampler0"HY% +4 "core" %wG 2 +3 ."FILTERING"Abs.stop +2 "ambient0"J5 +6 ."FILTERING"PhaseSeparator.stop +2 "carousel1"G6 +8 ."FILTERING"Intake.close +2 "sampler0"HI/ +150 "core" / +2 "ambient0"JZ% +5 "core" %# +5 #"FILTERING"Pressurized cartridge with 0.664ml to 15.0psi +2 #Isolated @25.2psia (11.7psi) +6 #Verifying cartridge holds at least 0.65..0.85 of 25.2psia +7 #Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi +4 "ambient0"J[% +6 "core" %& +44 "ambient0"J\\% +4 "core" %! +46 "ambient0"J]% +3 "core" % +47 "ambient0"J^% +3 "core" %@! +47 "ambient0"J_% +3 "core" %= +47 "ambient0"J`% +4 "core" %< +46 "ambient0"Ja% +4 "core" %5# +46 "ambient0"JG% +3 "core" %/, +47 "ambient0"JJ% +3 "core" %7. +47 "ambient0"J2% +4 "core" ʲ%-' +3 "carousel0"Fb% +3 "core" %[y, \ +4 #"FILTERING"Sampling 100.0ml +2 .SamplePump.setPosition! 0ml +4 "sampler0"H7 +6 ."FILTERING"Exhaust.open +2 "sampler0"Hi2 +149 "core" 2 +2 ."FILTERING"Intake.open +2 "sampler0"Hh1 +142 "core" 1 +8 "Can"k\\f.yshMu7dE + "ambient0"Jg% +4 "core" % +5 "ambient0"Je% +4 "core" %) +3 ."FILTERING"Abs.in :default +2 "ambient0"J>9 e  +6 ."FILTERING"Abs.hold +2 "ambient0"Jd+ +5 "core" + +3 ."FILTERING"PhaseSeparator.hold + "carousel1"Gc, +7 "core" , +2 "ambient0"Jf% +4 "core" %  +4 "sampler0"Hj% +4 "core" %Y  +3 "sampler0"Hp% +50 "core" %`  +15 ."FILTERING"SamplePump.in :start +2 "sampler0"H>^ m#(@@3 22Q +8 ."FILTERING"SamplePump.seek 100ml,6:04 +3 "sampler0"Hr'# +58 ."pumpStarted"SamplePump.configure :sample +3 "sampler0"H#d m#(@@3 22Q +224 Hs% +3 "core" %\ & ,ُ? {2 +3 "ambient0"Jt% +4 "core" % y +2 #"sampling"Cartridge 22 filtering 2/100ml at 0.76ml/s with 7.9psi vacuum +5994 "sampler0"Hu% +3 "core" % C n |2 +3 "ambient0"Jv% +4 "core" %5 z +2 #"sampling"Cartridge 22 filtering 45/100ml at 0.71ml/s with 13.2psi vacuum +505 "Can"l\\.ys@Z=E4 +5483 "sampler0"Hx% +3 "core" % 4? {2 +3 "ambient0"Jy% +4 "core" % y +2 #"sampling"Cartridge 22 filtering 87/100ml at 0.71ml/s with 13.0psi vacuum +1848 "core" ' +2 "sampler0"HN% +4 "core" %#ꊉ 2 +3 ."FILTERING"Abs.stop + "ambient0"J5 +7 ."FILTERING"PhaseSeparator.stop + "carousel1"G6 +9 ."closeIntake"Intake.close +3 "sampler0"HP/ +4 ."closeExhaust"Exhaust.close +9 "carousel0"FQ% +4 "sampler0"HR0 +9 "core" %[. \ +5 ."FILTERING"Toroid.in :hold +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :bypass +3 "valve0"DS( +103 "core" / +20 0 +231 ( +5 ."FILTERING"Toroid.in :default +2 "valve0"D? ֜2 +7 ."FILTERING"Toroid.seek :clear +2 "valve0"DU( +354 "core" ( +4 #"FILTERING"Sampled 100.0ml +3 "carousel0"Fq% +7 "core" %[-g \ +8 "valve0"D{% +4 "core" %ZGPOU +2 "syringe1"C}& +4 "core" &4m +3 "syringe1"Cm% +4 "core" %{ +3 "carousel0"F% +4 "core" %[. \ +3 "carousel0"FF% +5 "core" %[0- \ +117 ."PROCESSING"SPR.command "read_sequences" +65 .SPR.command "stats_on" +9 "carousel0"Fl% +3 "core" %[, \ +6 ."PROCESSING"SPR.command "start 0 1" +65 ."sourcePumpShutdown"Gate.power :sampler,:OFF +5 ."SPRlogger"SPR.start /var/log/esp/spr26aug0111h.spr.bz2 +197 ."PROCESSING"Handoff.select :waste +3 "valve0"D +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C% +6 "core" Á%} +45 "syringe1"C% +3 "core" Â%~v +47 "syringe1"C% +3 "core" Ã%x +47 "syringe1"C% +4 "core" Ä%I +46 "syringe1"C% +4 "core" Æ%~e +46 "syringe1"C% +3 "core" È%c +47 "syringe1"C % +3 "core" É%{f +47 "syringe1"C\ % +4 "core" Ê%yX +47 "syringe1"C % +3 "core" Ì%xh +47 "syringe1"C% +3 "core" Î%tx +47 "syringe1"C% +3 "core" Ð%vm +47 "syringe1"C% +3 "core" Ò%vp +47 "syringe1"C% +3 "core" Ô%u +47 "syringe1"C% +6 "core" Û%y} +6 "Can"l\\.yt$E*E̼ + #"PROCESSING"Syringe tare is 50% of full scale at 29C (drifted -1%) +4 .PinPort.in :locking +4 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"E( N +80 "core" ř( +4 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +8 ."posLyS2"Lysis2.in :fast +10 ."PROCESSING"Lysis1.seek 1250uL +4 "syringe0"B>d P\\k<2 +2 ."posArchive"Archive.seek :empty +4 "syringe1"C( +7 "syringe0"B( +5 ."posLyS2"Lysis2.seek 950uL +4 "syringe0"B' +246 "core" Ø( +258 —' +320 š( +3 "carousel0"F% +4 "core" Ɩ%'[ \ +6 #"PROCESSING"Evacuating puck with filter bubble point of 20psi +3 .PinPort.seek :locked +4 "valve1"E( N +8 "core" ŕ( +2 ."PROCESSING"Handoff.select :waste +59 .Plunge.seek :puckBottom +3 "valve0"D'B +133 "core" ĝ' +3 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C"% +8 "core" â%hc +43 "syringe1"C$% +3 "core" ä%o| +47 "syringe1"C%% +3 "core" å%o{ +47 "syringe1"C'% +3 "core" ç%ny +47 "syringe1"C)% +3 "core" é%rm +47 "syringe1"C*% +3 "core" ê%o` +48 "syringe1"C+% +5 "core" ë%nO +44 "syringe1"C~% +3 "core" %kK +48 "syringe1"C,% +3 "core" ì%8O +47 "syringe1"C-% +3 "core" í%g< +47 "syringe1"C.% +3 "core" î%iI +47 "syringe1"C/% +3 "core" ï%_Y +47 "syringe1"C% +3 "core" Å%eT +47 "syringe1"C % +3 "core" Í%]d +47 "syringe1"C% +3 "core" Ï%cg +47 "syringe1"C% +3 "core" Ñ%`o +47 "syringe1"C % +3 "core" Ë%c~ +4 #"PROCESSING"Air syringe pumping up to 1 strokes to reach 4psi +2 #Air stroke #1 of 1 +4 "syringe1"C% +7 "core" Ç%g] +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N X P]z<2 +6 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C:' +517 "core" ú' +3 "syringe1"C<% +4 "core" ü%#S{ < +2 #"PROCESSING"0.0psi after pressurize +3 .Air.in :compress +4 "syringe1"C>N X P]zP2 +6 "Can"o\\f.yt:FE4 +3 ."PROCESSING"Air.seek :evac +2 "syringe1"C&'| +541 "core" æ'ߏp:  jPm +4 "syringe1"C=% +5 "core" ý%ߏ: jPb +2 #"PROCESSING"Reached 1.6 of 4.0psi after pumping 1711uL (0.96 strokes) +2 .Air.in :default +4 "syringe1"C>d P]z<2 +13 ."PROCESSING"Air.hold +3 "syringe1"C;+ +4 "core" û+ +4 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +8 "syringe1"C3% +7 "core" ó%ߏ:\ P p +43 "syringe1"C9% +3 "core" ù%ߏ: e +47 "syringe1"C% +3 "core" Ü%ߏ:G h +47 "syringe1"C#% +4 "core" ã%ߏ:  Z +46 "syringe1"C!% +3 "core" á%ߏ:| g +47 "syringe1"C % +3 "core" à%ߏ: ` +47 "syringe1"C% +3 "core" Ó%ߏ: 6 Z +47 "syringe1"C0% +4 "core" ð%ߏ:$H V +47 "syringe1"C>% +3 "core" þ%ߏ:G b +47 "syringe1"C8% +4 "core" ø%ߏ:#/ W +46 "syringe1"C5% +4 "core" õ%ߏ: g +46 "syringe1"C% +3 "core" Þ%ߏ: ` +49 "syringe1"C% +5 "core" ß%ߏ:( R +43 "syringe1"C1% +3 "core" ñ%ߏ:)~ Q +47 "syringe1"C6% +4 "core" ö%ߏ:m ` +46 "syringe1"Ck% +4 "core" %ߏ: v Z +46 "syringe1"C4% +3 "core" ô%ߏ: ] +47 "syringe1"C?% +3 "core" ÿ%ߏ: [ +47 "syringe1"CB% +4 "core" %ߏ:! Y +46 "syringe1"CD% +4 "core" %ߏ:# W +46 "syringe1"CE% +3 "core" %ߏ:$ V +47 "syringe1"CA% +3 "core" %ߏ:  Z +47 "syringe1"CM% +4 "core" %ߏ:. L +46 "syringe1"CC% +3 "core" %ߏ:$~ V +48 "syringe1"CL% +7 "core" %ߏ:t g +2 #"PROCESSING"Stabilized at 0.1psi after evac to waste + .Handoff.select :blocked +3 "valve0"D0 +52 ."PROCESSING"Plunge.seek :puckTop +3 "valve0"DK': +84 "core" ' +5 #"PROCESSING"Air syringe pumping up to 8 strokes to reach 20psi +2 #Air stroke #1 of 8 +2 "syringe1"CW% +10 "core" %ߏ: _ +3 ."PROCESSING"Air.in :compress +2 "syringe1"C>N  P]zP2 +6 ."PROCESSING"Air.seek :evac +3 "syringe1"CX'| +120 "core" ' +2 "syringe1"C7% +4 "core" ÷%|h4 oPR +2 #"PROCESSING"1.8psi after compress +3 "syringe1"CY% +7 "core" %|4 b +43 "syringe1"CI% +4 "core" %|4 T +46 "syringe1"CZ% +3 "core" %|4 R +47 "syringe1"C[% +4 "core" %|4{B L +46 "syringe1"C\\% +3 "core" %|4yK Y +47 "syringe1"C]% +3 "core" %|4zX V +47 "syringe1"CT% +3 "core" %|4z} ` +47 "syringe1"CV% +3 "core" %|4yw X +48 "syringe1"C^% +3 "core" %|4h X +46 "syringe1"C_% +3 "core" %|4tw N +49 "syringe1"C`% +8 "core" %|4zp Z +40 "syringe1"Ca% +3 "core" %|4w [ +47 "syringe1"CG% +3 "core" %|4t ] +47 "syringe1"CJ% +3 "core" %|4t [ +47 "syringe1"C2% +3 "core" ò%|4u T +47 "syringe1"Cb% +3 "core" %|4 Z +48 "syringe1"Ci% +3 "core" %|4x Q +47 "syringe1"Ch% +3 "core" %|4r _ +47 "syringe1"Cg% +3 "core" %|4s ` +47 "syringe1"Ce% +3 "core" %|4u W +47 "syringe1"Cf% +4 "core" %|4v d +46 "syringe1"Cj% +3 "core" %|4t Z +47 "syringe1"Cp% +4 "core" %|4t a +2 #"PROCESSING"3.1psi after compress (stable) +3 .Air.in :gulp +2 "syringe1"C>N  P]z<2 +8 ."PROCESSING"Air.seek :topVent +2 "syringe1"Cs'6 +639 "core" ' +2 "syringe1"Ct% +4 "core" %62 <z +3 #"PROCESSING"3.0psi after gulp +2 "syringe1"CH% +11 "core" %6b +39 "syringe1"Cu% +7 "core" %6c ~ +43 "syringe1"Cv% +4 "core" %6g | +47 "syringe1"Cx% +4 "core" %6d } +45 "syringe1"Cy% +4 "core" %6d +46 "syringe1"CN% +3 "core" %6a +48 "syringe1"CQ% +3 "core" %6d +47 "syringe1"CP% +3 "core" %6c +47 "syringe1"CR% +5 "core" %6c  +45 "syringe1"Cd% +3 "core" %6] | +47 "syringe1"Cc% +4 "core" %6\\ | +46 "syringe1"C@% +4 "core" %6e +46 "syringe1"CS% +5 "core" %6] z +4 #"PROCESSING"3.2psi after gulp (stable) +2 #Air stroke #2 of 8 +2 "syringe1"Cq% +12 "core" %6_ +3 ."PROCESSING"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +7 ."PROCESSING"Air.seek :pressurized +3 "syringe1"C{' +630 "core" ' +2 "syringe1"C}% +4 "core" %CS < +3 #"PROCESSING"3.4psi after pressurize +8 .Air.in :compress +3 "syringe1"C>N  P]zP2 +8 ."PROCESSING"Air.seek :evac +2 "syringe1"Cm'| +654 "core" ' +2 "syringe1"C% +4 "core" %|4 iPh +3 #"PROCESSING"14.3psi after compress +3 "syringe1"CF% +7 "core" %|4 m +43 "syringe1"Cl% +3 "core" %|4 p +47 "syringe1"C% +4 "core" Á%|4 g +46 "syringe1"C% +4 "core" Â%|4 e +46 "syringe1"C% +3 "core" Ã%|4. [ +47 "syringe1"C% +3 "core" Ä%|4I Y +47 "syringe1"C% +3 "core" Æ%|4Q ] +8 '|4 \\ +4 "syringe1"C% +4 "core" È%|4c d +2 #"PROCESSING"Reached 16.6 of 20.0psi after pumping 3551uL (2.00 strokes) +2 .Air.in :default +5 "syringe1"C>d P]z<2 +6 ."PROCESSING"Air.hold +2 "syringe1"C + +6 "core" É+ +3 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"D\ 'B +84 "core" Ċ' +3 ."PROCESSING"delay 3 seconds +303 .Handoff.select :waste +2 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C % +5 "core" Ì%|4 [ +46 "syringe1"C% +3 "core" Î%|4/ X +47 "syringe1"C% +3 "core" Ð%|4 Y +47 "syringe1"C% +3 "core" Ò%|4 S +47 "syringe1"C% +3 "core" Ô%|4n S +47 "syringe1"C% +3 "core" Û%|4" \\ +47 "syringe1"CO% +3 "core" %|4. Z +47 "syringe1"Cz% +3 "core" %|4 Z +47 "syringe1"Co% +4 "core" %|4 X +46 "syringe1"Cn% +4 "core" %|4 b +46 "syringe1"C% +4 "core" Ö%|4 W +46 "syringe1"C"% +5 "core" â%|4 a +45 "syringe1"C$% +5 "core" ä%|4 U +45 "syringe1"C% +4 "core" Ù%|4 Y +47 "syringe1"C%% +4 "core" å%|4 W +45 "syringe1"C'% +4 "core" ç%|4 ] +46 "syringe1"C|% +4 "core" %|4~ V +46 "syringe1"C)% +4 "core" é%|4 ] +46 "syringe1"C*% +4 "core" ê%|4 R +46 "syringe1"C+% +5 "core" ë%|4 M +45 "syringe1"C~% +4 "core" %|4} Y +46 "syringe1"C,% +4 "core" ì%|4 W +46 "syringe1"C-% +4 "core" í%|4 P +46 "syringe1"C.% +4 "core" î%|4 Y +3 ."PROCESSING"delay 1:00 +4155 "Can"i\\.yt9`KE4 +1847 ."PROCESSING"Handoff.select :blocked +3 "valve0"D0 +51 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-1.50psi + "syringe1"C/% +6 "core" ï%|4v _ +45 "syringe1"C% +3 "core" Å%|4 Y +47 "syringe1"C % +4 "core" Í%|4 \\ +46 "syringe1"C% +5 "core" Ï%|4 W +45 "syringe1"C% +6 "core" Ñ%|4 T +44 "syringe1"C % +3 "core" Ë%|4 R +47 "syringe1"C% +3 "core" Ç%|4 _ +47 "syringe1"C(% +4 "core" è%|4 Q +46 "syringe1"C<% +4 "core" ü%|4 V +46 "syringe1"C:% +4 "core" ú%|4 X +46 "syringe1"C=% +3 "core" ý%|4 [ +47 "syringe1"C3% +4 "core" ó%|4 R +46 "syringe1"C9% +3 "core" ù%|4 K +47 "syringe1"C% +3 "core" Ü%|4~ S +47 "syringe1"C#% +3 "core" ã%|4 W +47 "syringe1"C!% +4 "core" á%|4{ S +46 "syringe1"C % +3 "core" à%|4w W +47 "syringe1"C&% +3 "core" æ%|4x Z +47 "syringe1"C% +3 "core" Ó%|4y X +47 "syringe1"C0% +4 "core" ð%|4 Y +46 "syringe1"C>% +4 "core" þ%|4~ Z +47 "syringe1"C8% +5 "core" ø%|4{ S +44 "syringe1"C5% +4 "core" õ%|4z ^ +46 "syringe1"C% +3 "core" Þ%|4} W +47 "syringe1"C% +3 "core" ß%|4z _ +47 "syringe1"C1% +3 "core" ñ%|4{ Y +47 "syringe1"C6% +3 "core" ö%|4w W +47 "syringe1"Ck% +4 "core" %|4t R +46 "syringe1"C4% +4 "core" ô%|4v W +46 "syringe1"C?% +4 "core" ÿ%|4u W +46 "syringe1"CB% +4 "core" %|4t Q +46 "syringe1"CD% +4 "core" %|4u \\ +46 "syringe1"CE% +4 "core" %|4n R +46 "syringe1"CA% +3 "core" %|4s ] +47 "syringe1"CM% +3 "core" %|4j W +47 "syringe1"CC% +3 "core" %|4p a +47 "syringe1"CL% +51 "core" %|4p X +6 "syringe1"CW% +4 "core" %|4q S +41 "syringe1"C7% +4 "core" ÷%|4p b +44 "syringe1"CY% +4 "core" %|4o R +47 "syringe1"CI% +3 "core" %|4o W +47 "syringe1"C% +3 "core" Ý%|4h V +47 "syringe1"CZ% +3 "core" %|4o S +47 "syringe1"C[% +3 "core" %|4k ] +47 "syringe1"C;% +3 "core" û%|4h W +47 "syringe1"C\\% +3 "core" %|4h W +3 ."PROCESSING"Handoff.select :waste +3 "valve0"D +53 #"PROCESSING"0.3psi at evacEnd +3 .Plunge.seek :pTop +4 "valve0"D]': +84 "core" ' +2 ."PROCESSING"delay 5 seconds +503 .Air.in :glide +2 "syringe1"C>d  P]z<2 +6 ."PROCESSING"Air.seek :fullVent +2 "syringe1"CT'݇ +162 "core" ' +3 ."PROCESSING"delay 2 seconds +202 .Plunge.seek :pBottom +2 "valve0"DV'B +86 "core" ' + #"PROCESSING"Seawater evacuated, Pressure above/below filter released. +3 .Plunge.seek :locked +5 "valve0"D^'Z +127 "core" ' +2 ."PROCESSING"Handoff.select :waste +4 "syringe1"C_& +10 "core" &S} 9 +3 ."PROCESSING"Lysis1.in :slow +2 "syringe1"C?d P[<2 +7 ."PROCESSING"Lysis1.seek 950uL +3 "syringe1"C`( +1406 "core" ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select :blocked +4 "valve0"D0 +49 ."PROCESSING"Plunge.seek :puckTop +3 "valve0"Da': +87 "core" ' +2 "syringe1"CG& +3 "core" &zs~ a +4 ."PROCESSING"Lysis1.seek 350uL +3 "syringe1"CJ( +2797 "core" ( +2 ."PROCESSING"delay 3 seconds +303 "syringe1"C2% +5 "core" ò%݇/O t +5 ."PROCESSING"Air.in mix with 2.79934 parts Lysis1 +4 "syringe1"C> wk+ P]z<2 +7 Cb% +3 "core" %݇/W x +3 "syringe1"Ci& +6 "core" &GO{ +56 ."Airmixer"Air.seek :evac +4 ."PROCESSING"Lysis1.seek 190uL +9 "syringe1"Ch'| +3 Cg( +757 "core" ' +4 ( +3 ."PROCESSING"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."PROCESSING"Air.seek :full +3 "syringe1"Ce'6 +469 "Can"j[.ys?uPhF4 +178 "core" ' +3 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"Cf( +895 "core" ( +2 "valve0"Dj% +5 "core" %:7Oa +2 "syringe1"Cp% +4 "core" %6;E x +2 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @13.6psi +3 .Plunge.seek :puckBottom +4 "valve0"DX'B +85 "core" ' +2 "valve0"Dt% +4 "core" %Bx(=Xy - +3 "syringe1"CH% +3 "core" %6o1 v +3 #"PROCESSING"Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @12.1psi +2 #Polling Can every 2:00 +57 "Can"m\\.ytDQF] +2 ."PROCESSING"PuckHeater.configure :default #ambient 28.6C +2 "Cartridge" \ :D H  +6 ."PROCESSING"PuckHeater.seek 81.3C +4 "Cartridge" u  +5 v9 +5 "cartridge" 9( +104 "Cartridge" x9 +3 "cartridge" 9(}d +105 "Cartridge" y9 +4 "cartridge" 9)d +103 "Cartridge" N9 +3 "cartridge" 9,V d +104 "Cartridge" Q9 +4 "cartridge" 9/ d +103 "Cartridge" P9 +3 "cartridge" 93\ d +104 "Cartridge" R9 +3 "cartridge" 964d +103 "Cartridge" d9 +4 "cartridge" 98d +103 "Cartridge" c9 +4 "cartridge" 9;Od +104 "Cartridge" @9 +4 "cartridge" 9=#d +105 "Cartridge" S9 +4 "cartridge" 9?'d +103 "Cartridge" q9 +3 "cartridge" 9A}+d +103 "Cartridge" s9 +4 "cartridge" 9CC0d +107 "Cartridge" }9 +4 "cartridge" 9E 4d +103 "Cartridge" {9 +3 "cartridge" 9F 8d +104 "Cartridge" 9 +4 "cartridge" 9HL =d +103 "Cartridge" F9 +4 "cartridge" 9I Ad +106 "Cartridge" l9 +3 "cartridge" 9KL8Ed +103 "Cartridge" 9 +3 "cartridge" 9L8Jd +104 "Cartridge" 9 +3 "cartridge" 9N'8Nd +103 "Cartridge" 9 +4 "cartridge" 9O8Rd +103 "Cartridge" 9 +3 "cartridge" 9PeVd +104 "Cartridge" 9 +3 "cartridge" 9R8[d +103 "Cartridge" 9 +5 "cartridge" 9Sr _d +105 "Cartridge" m9 +5 "cartridge" 9Tecd +104 "Cartridge" K9 +3 "cartridge" 9Uhd +151 "Cartridge" 9 +3 "cartridge" 9Wend +104 "Cartridge" 9 +3 "cartridge" 9Xrd +106 "Cartridge" 9 +4 "cartridge" 9Ywd +103 "Cartridge" 9 +3 "cartridge" 9[{d +104 "Cartridge" 9 +3 "cartridge" 9\\8d +104 "Cartridge" 9 +3 "cartridge" 9],d +103 "Cartridge" O9 +4 "cartridge" 9^Ed +103 "Cartridge" z9 +5 "cartridge" 9_Wd +103 "Cartridge" o9 +3 "cartridge" 9`ad +106 "Cartridge" n9 +5 "cartridge" 9ad +103 "Cartridge" 9 +3 "cartridge" 9buc +104 "Cartridge" "9 +3 "cartridge" 9cyd +103 "Cartridge" $9 +4 "cartridge" 9dnd +103 "Cartridge" 9 +4 "cartridge" 9e^d +103 "Cartridge" %9 +3 "cartridge" 9fFd +104 "Cartridge" '9 +3 "cartridge" 9gQd +103 "Cartridge" |9 +4 "cartridge" 9hUc +105 "Cartridge" )9 +4 "cartridge" 9i6d +104 "Cartridge" *9 +3 "cartridge" 9j:d +103 "Cartridge" +9 +3 "cartridge" 9k(Dc +104 "Cartridge" ~9 +3 "cartridge" 9kDd +104 "Cartridge" ,9 +6 "cartridge" 9lDd +103 "Cartridge" -9 +5 "cartridge" 9mqd +103 "Cartridge" .9 +4 "cartridge" 9nqd +104 "Cartridge" /9 +3 "cartridge" 9oqd +104 "Cartridge" 9 +3 "cartridge" 9p|qd +103 "Cartridge" 9 +4 "cartridge" 9qXqd +103 "Cartridge" 9 +4 "cartridge" 9r8c +104 "Cartridge" 9 +3 "cartridge" 9sd +103 "Cartridge" 9 +3 "cartridge" 9sd +104 "Cartridge" 9 +3 "cartridge" 9td +103 "Cartridge" (9 +4 "cartridge" 9ud +104 "Cartridge" <9 +4 "cartridge" 9vmd +104 "Cartridge" :9 +3 "cartridge" 9wbd +103 "Cartridge" =9 +3 "cartridge" 9x d +106 "Cartridge" 39 +3 "cartridge" 9xd +104 "Cartridge" 99 +3 "cartridge" 9yd +103 "Cartridge" 9 +4 "cartridge" 9z d +103 "Cartridge" #9 +3 "cartridge" 9{d$d +104 "Cartridge" !9 +6 "cartridge" 9|d +104 "Cartridge" 9 +4 "cartridge" 9|$d +120 "Cartridge" &9 +4 "cartridge" 9}$d +103 "Cartridge" 9 +4 "cartridge" 9~#c +104 "Cartridge" 09 +3 "cartridge" 9R$'c +103 "Cartridge" >9 +4 "cartridge" 9!P+c +105 "Cartridge" 89 +4 "cartridge" 9P0c +108 "Cartridge" 59 +3 "cartridge" 9P4c +104 "Cartridge" 9 +3 "cartridge" 9P9b +104 "Cartridge" 9 +3 "cartridge" 9?}=a +104 "Cartridge" 19 +3 "cartridge" 9PA_ +103 "Cartridge" 69 +4 "cartridge" 9}E] +4 ."PROCESSING"PuckHeater.hold 2:00,81.3C +8 "Cartridge" k }c +5 "cartridge" F +2432 "Can"k[.ys6XF-4 +3 k[.ys6XF-6 +9566 "cartridge" +3 #"PROCESSING"Polling Can every 2:00 +53 "Can"k[.ytE]`FEX +3 ."PROCESSING"PuckHeater.configure :default #ambient 28.7C +2 "Cartridge" \ :D H  +7 ."PROCESSING"PuckHeater.seek 80.2C +4 "Cartridge" 4  +4 ?9 +5 "cartridge" 9 +5 ."PROCESSING"PuckHeater.hold 2:00,80.2C +7 "Cartridge" B  +5 "cartridge"  +2294 "Can"k\\#.yt(^EFK4 +3 k\\#.ys(^EFK6 +9696 "cartridge" +4 #"PROCESSING"Polling Can every 2:00 +52 "Can"m\\.ytBbFc +3 ."PROCESSING"PuckHeater.configure :default #ambient 28.6C +2 "Cartridge" \ :D H  +6 ."PROCESSING"PuckHeater.hold 8:00,79.2C +8 "Cartridge" D Bχ +2186 "Can"j\\?.yt&bFi4 +3 j\\?.yt&BbFi6 +11954 l\\.yt%gIF4 +4 l\\.yt&(gIF7 +11954 k[.yt%vkF4 +3 k[.yt%kF6 +11954 l\\}.yt%pF4 +4 l\\}.yt %pF6 +9914 "cartridge" +3 "syringe1"CE% +4 "core" %6 } +3 #"PROCESSING"Lysate cooked @9.1psi +2 .Plunge.seek :puckTop +5 "valve0"DA': +86 "core" ' +6 #"PROCESSING"Air syringe pumping up to 5 strokes to reach 40psi + #Air stroke #1 of 5 +4 "syringe1"CM% +8 "core" %6 x +4 ."PROCESSING"Air.in :pressurize +5 "syringe1"C>@N E P]z<2 +4 ."PROCESSING"Air.seek :pressurized +3 "syringe1"CC' +629 "core" ' +2 "syringe1"CL% +4 "core" %(S <| +2 #"PROCESSING"9.0psi after pressurize +3 .Air.in :compress +2 "syringe1"C>N E P]zP2 +10 ."PROCESSING"Air.seek :evac +3 "syringe1"CW'| +573 "core" '?p8xz iPn +5 "syringe1"C7% +4 "core" ÷%?8z$ lPl +2 #"PROCESSING"Reached 41.2 of 40.0psi after pumping 1730uL (0.97 strokes) +3 .Air.in :default +3 "syringe1"C>d P]z<2 +7 ."PROCESSING"Air.hold +2 "syringe1"CY+ +5 "core" + +3 ."PROCESSING"delay 2 seconds +203 .Air.in :fast +2 "syringe1"C> P]z<2 +6 ."PROCESSING"Air.seek :full +3 "syringe1"CI'6 +427 "Can"k[N.ys?uF4 +205 "core" ' +3 ."PROCESSING"Plunge.seek :puckBottom +2 "valve0"D'B +86 "core" ĝ' +2 "syringe1"CZ& +4 "core" &*k` +3 ."PROCESSING"Lysis1.seek 138uL +51 "syringe1"C[(# +653 "core" ( +2 ."PROCESSING"delay 2 seconds +205 .Plunge.seek :puckTop +3 "valve0"D;': +85 "core" Ļ' +2 ."PROCESSING"Lysis1.seek :empty +3 "syringe1"C\\( +655 "core" ( +3 ."PROCESSING"Plunge.seek :puckBottom +3 "valve0"D\ 'B +87 "core" Ċ' +3 "carousel0"F % +11 "core" Ɖ%[ \ +4 "valve0"D_% +6 "core" %B(COb +4 "syringe1"C]% +6 ."PROCESSING"Lysis1.in :extract +3 "syringe1"C?d 'DJ P[#2 +5 "core" %6Y$ } +6 ."PROCESSING"Lysis1.seek 675uL,nil +3 "syringe1"CV( +6 C& +3 "core" Ø&4*fY' # +3 "syringe1"CG& +4 "core" &*y # +8 "syringe1"C^& +5 "core" &* # +17 "syringe1"C`& +3 "core" &F #, # +4 "syringe1"C2% +4 "core" ò%6  } +7 "syringe1"Cb& +4 "core" &ݡ . # +16 "syringe1"Ci& +4 "core" & 0z # +16 "syringe1"CT& +4 "core" &d 2 # +4 "syringe1"CJ% +3 "core" %6  { +9 "syringe1"Ch& +4 "core" & 4w" # +16 "syringe1"Cg& +4 "core" &' #6\\ # +16 "syringe1"Cj& +5 "core" &߉ 8BU # +3 "syringe1"Cp% +4 "core" %6 } +8 "syringe1"Ct& +4 "core" & :"6 # +17 "syringe1"CH& +3 "core" &N <\  # +16 "syringe1"Cv& +3 "core" & = # +9 "syringe1"Cx% +4 "core" %6 +5 "syringe1"Ca& +3 "core" &  ?* # +16 "syringe1"Cy& +5 "core" &r AK # +15 "syringe1"CN& +4 "core" & Cr # +5 "syringe1"CQ% +3 "core" %63 y +8 "syringe1"CP& +3 "core" &6 EF #) +18 "syringe1"CR& +6 "core" & G #& +13 "syringe1"Cd& +3 "core" & I'm #) +5 "syringe1"Cc% +7 "core" %62 w +3 "syringe1"C@% +5 "core" %6  +2 ."pump"Air.in :pressurize +2 "syringe1"C>@N  P]z<2 +12 CS& +3 ."pump"Air.seek :pressurized +6 "syringe1"Cq' +7 "core" &2MX' #. +3 "syringe1"Cs& +9 "core" &3NΉ #* +4 "syringe1"C}& +7 "core" &VLOΉ[ #6 +6 "syringe1"C{& +3 "core" &JQ lj #! +13 "syringe1"C& +3 "core" &MRaΈ #- +17 "syringe1"CF& +4 "core" &CPStɈ #+ +16 "syringe1"Cl& +4 "core" &"TTĈ #$ +16 "syringe1"C& +4 "core" Á&(^T-ƈw # +16 "syringe1"C& +4 "core" Â&(=T.o  +16 "syringe1"C& +4 "core" Ã&(6T' # +16 "syringe1"C& +4 "core" Ä&(@T{ - +16 "syringe1"C& +4 "core" Æ&(AT$r & +16 "syringe1"C& +4 "core" È&(BT*u +16 "syringe1"Cm& +5 "core" &(FT-y  +15 "syringe1"CK& +5 "core" &(FT { * +15 "syringe1"C & +4 "core" Ì&(ET& $ +16 "syringe1"C& +5 "core" Î&(IT%| % +16 "syringe1"C& +5 "core" Ð&(KT,n  +14 "syringe1"C& +3 "core" Ò&(ST*h +17 "syringe1"C& +3 "core" Ô&(KT(ƈf " +17 "syringe1"C& +3 "core" Û&(OTÈS / +17 "syringe1"CO& +3 "core" &(PTЈZ + +17 "syringe1"Cz& +3 "core" &(ST(Ĉ` " +17 "syringe1"Co& +3 "core" &(UT&ψ] $ +17 "syringe1"Cn& +3 "core" &(YTȈQ - +17 "syringe1"C& +3 "core" Ö&(ZT1ˆ=  +17 "syringe1"C"& +4 "core" â&(aT)̈H ! +16 "syringe1"C$& +4 "core" ä&(^T.ňC  +17 "syringe1"C& +3 "core" Ù&(`T+ň[  +16 "syringe1"C%& +4 "core" å&(eTɈF / +16 "syringe1"C'& +4 "core" ç&(hT!Lj( ) +17 "syringe1"C|& +6 "core" &(eT$H & +13 "syringe1"C)& +50 "core" é&(eT$L & + ' +4 "syringe1"C*% +6 C+& +3 "core" ê%SS +2 ë&(T,q  + #"pump"10.9psi after pressurize +5 "syringe1"C~& +12 ."pump"Air.in :compressLysate +8 "syringe1"C>dN  P]z<2 +7 "core" &(T'q # +3 ."pump"Air.seek :evac +3 "syringe1"C,'| +6 C-& +7 "core" í&(4T/}Z  +2 "syringe1"C.& +4 "core" î&(8T#Ȉ` ' +4 "syringe1"C/& +4 "core" ï&(&T3c 7 +9 "syringe1"C& +3 "core" Å&(?T&͈P $ +17 "syringe1"C & +3 "core" Í&(ET(L " +17 "syringe1"C& +3 "core" Ï&(ET1  +17 "syringe1"C& +3 "core" Ñ&(NT%k % +17 "syringe1"C & +3 "core" Ë&(LT/Ĉ`  +17 "syringe1"C& +4 "core" Ç&(JT+Lje  +16 "syringe1"C(& +3 "core" è&(7T(a " +17 "syringe1"C<& +3 "core" ü&(4T( " +17 "syringe1"C:& +3 "core" ú&(@T"Ĉg ( +17 "syringe1"C=& +3 "core" ý&('T!~ ) +17 "syringe1"C3& +3 "core" ó&(OT% % +19 "syringe1"C9& +5 "core" ù&(ST' # +13 "syringe1"C& +3 "core" Ü&(HT!ƈ ) +17 "syringe1"C#& +3 "core" ã&(PT,Ȉ  +17 "syringe1"C!& +3 "core" á&(TT%ň % +17 "syringe1"C & +3 "core" à&(,T( " +17 "syringe1"C&& +3 "core" æ&(8T) ! +17 "syringe1"C& +3 "core" Ó&(RT( " +17 "syringe1"C0& +3 "core" ð&(`T% % +17 "syringe1"C>& +3 "core" þ&(RT) ! +17 "syringe1"C8& +3 "core" ø&(WT* +17 "syringe1"C5& +3 "core" õ&(VT.  +17 "syringe1"C& +3 "core" Þ&(RT$# & +17 "syringe1"C& +3 "core" ß&(CT($ " +17 "syringe1"C1& +4 "core" ñ&(9T*L +16 "syringe1"C6& +4 "core" ö&(PT%- % +16 "syringe1"Ck& +4 "core" &(XT'B # +16 "syringe1"C?& +3 "core" ÿ&(WT*y +17 "syringe1"CB& +3 "core" &(UT.g  +17 "syringe1"Cu& +3 "core" &(aT/p  +19 "syringe1"C4& +3 "core" ô&(6T*g +15 "syringe1"CD& +3 "core" &([T$ & +17 "syringe1"CE& +3 "core" &(FT* +17 "syringe1"CM& +3 "core" &(>T" ( +17 "syringe1"CX& +3 "core" &(`T) ! +17 "syringe1"Ce& +3 "core" &(gT! ) +17 "syringe1"CL& +3 "core" &(TT( " +17 "syringe1"CC& +3 "core" &(gT+  +17 "syringe1"C7& +3 "core" ÷&(hT% % +17 "syringe1"CW& +3 "core" &(]T*% +17 "syringe1"CY& +3 "core" &(XT%2 % +17 "syringe1"CZ& +3 "core" &(\\T$[ & +17 "syringe1"CA& +3 "core" &(aT*u +17 "syringe1"Cf& +3 "core" &(DT#y ' +18 "syringe1"C& +6 "core" Ý&(aT$ & +13 "syringe1"C[& +3 "core" &(hT,  +17 "syringe1"C & +3 "core" É&(?T* +17 "syringe1"C_& +3 "core" &(jT,  +17 "syringe1"C]& +3 "core" &(dT1  +18 "syringe1"C& +3 "core" Ø&(fT+3  +16 "syringe1"CG& +3 "core" &(AT, # +17 "syringe1"C^& +3 "core" &( zT.Q ~# +17 "syringe1"C`& +3 "core" &+ PT6 ## +17 "syringe1"C2& +3 "core" ò&0=gTh싇 }# +17 "syringe1"Cb& +3 "core" &:]tT ~# +17 "syringe1"Ci& +3 "core" &G}T }# +17 "syringe1"CT& +3 "core" &Y|UA # +17 "syringe1"CJ& +4 "core" &p~U }# +16 "syringe1"Ch& +3 "core" &V< ~# +17 "syringe1"Cg& +3 "core" & |V} # +17 "syringe1"C;& +3 "core" û&"cWZ ~# +17 "syringe1"Cj& +4 "core" &5kX`] ~# +16 "syringe1"Cp& +3 "core" &C{Y"? ~# +17 "syringe1"Ct& +5 "core" &AI|Y! |# +15 "syringe1"CH& +3 "core" &gOZ6 # +17 "syringe1"Cv& +3 "core" &Y|[ }# +17 "syringe1"Cx& +3 "core" &n\\j ~# +17 "syringe1"Ca& +3 "core" &Y]X ~# +17 "syringe1"Cy& +3 "core" & q^i }# +17 "syringe1"CN& +3 "core" &[q_Z ~# +17 "syringe1"CQ& +3 "core" &%a` # +17 "syringe1"C\\& +3 "core" &b6 }# +17 "syringe1"CP& +3 "core" &c1( }# +2 ( +2 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +5 ."pump"Air.hold +3 "syringe1"CR+ +12 "core" ì'AT < + + +3 "syringe1"Cd& +5 "core" &c6 +3 "syringe1"Cc% +51 "core" %As +2 #"PROCESSING"Pulled 675uL lysate @13.4psi +3 "valve0"D@% +8 "core" %B(LOp +18 "syringe1"CS% +7 ."PROCESSING"Lysis1.seek 800uL,nil +3 "syringe1"Cs(% +7 "core" %AtG +2 "syringe1"C}% +5 C{& +6 "core" %AL + &c8>^ +2 "syringe1"C& +3 ."pump"Air.in :compressLysate +4 "syringe1"C>dN  P]z<2 +14 ."pump"Air.seek :evac +3 "syringe1"CF'| +6 "core" &cCxr +3 "syringe1"Cl& +4 "core" &[c> +3 "syringe1"C& +8 "core" Á&ac? +6 "syringe1"C& +3 "core" Â&UcM +15 "syringe1"C& +3 "core" Ã&[cB +17 "syringe1"C& +3 "core" Ä&_cL +17 "syringe1"C& +4 "core" Æ&5c@ +16 "syringe1"C& +3 "core" È&_c5 +17 "syringe1"Cm& +3 "core" &Jc= +17 "syringe1"CK& +3 "core" &>c7 +17 "syringe1"C & +3 "core" Ì&}c<% ~# +18 "syringe1"C& +5 "core" Î& rc>< }# +15 "syringe1"CI& +6 "core" &lcJf }# +13 "syringe1"C& +4 "core" Ð&4rc`f |# +16 "syringe1"C& +3 "core" Ò& GtcX # +17 "syringe1"C& +3 "core" Ô&*bwcڋz ~# +17 "syringe1"C& +3 "core" Û&7ysdr |# +17 "syringe1"CO& +3 "core" &HZd[苑 }# +17 "syringe1"Cz& +3 "core" &[|d݋y |# +17 "syringe1"Co& +3 "core" &qZe=Z # +17 "syringe1"Cn& +3 "core" &ne_ # +18 "syringe1"C& +6 "core" Ö&TfTK # +13 "syringe1"C"& +3 "core" â&f֋` ~# +17 "syringe1"C$& +3 "core" ä&~g3 # +17 "syringe1"C& +3 "core" Ù&whߋ4 ~# +17 "syringe1"C%& +3 "core" å& }he {# +17 "syringe1"C'& +3 "core" ç&Bcisڋ # +17 "syringe1"C|& +3 "core" &ewj% # +17 "syringe1"C)& +4 "core" é&&~j$ }#l +16 "syringe1"C*& +3 "core" ê&2ik6 ~#n +17 "syringe1"C+& +3 "core" ë&=loފ |#b +22 "syringe1"C~& +4 "core" &Xmf }#r +11 "syringe1"C-& +3 "core" í&%Fm ~# +4 ( +2 ."pump"Air.in :default +2 "syringe1"C>d P]z<2 +5 ."pump"Air.hold +2 "syringe1"C.+ +7 "core" 'ߛp:Gh

d  P]z<2 +7 ."PROCESSING"Air.seek :fullVent +3 "syringe1"C '݇ +235 "core" Í' +2 ."PROCESSING"Handoff.select :waste +3 "valve0"D +50 #"PROCESSING"Recovered 800uL lysate @15.1psi +2 .delay 3 seconds +304 "syringe1"C% +3 "core" Ï%݇/_ x +2 #"PROCESSING"After 3 delay Recovered 800uL lysate @0.5psi +3 .Plunge.seek :locked +5 "valve0"D'Z +128 "core" đ' +3 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +7 B % +3 "core" ‹%{ e +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +7 C& +3 "core" Ç&%n)) K +59 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 550uL +8 "syringe0"B('| +2 "syringe1"C<( +3488 "core" ¨' +4 ü( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +50 ."PROCESSING"SPR.command "start 2 14" +144 #SPR stabilized + "syringe1"C:& +7 "core" ú&X~ +2 "syringe0"B=% +6 "core" ½%|fsg w +2 ."PROCESSING"delay 20 seconds +1368 "Can"k[.yt1|wF4 +634 ."PROCESSING"SPR.command "start 2 0" +358 #Waiting up to 11:00 for SPR to enter state 1 +10965 "Can"o[.yt/EG4 +844 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B3% +7 "core" ³%|f s +3 "syringe1"C9& +7 "core" ù&X~ +53 ."Lysis2mixer"Lysis2.seek 400uL +5 ."PROCESSING"Lysis1.seek 250uL +7 "syringe0"B' +2 "syringe1"C#( +4181 "core" œ' +6 ã( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +287 #Waiting up to 1:00:00 for SPR to enter state 7 +4911 "Can"k[.ys/?bG;4 +11959 m[.yt/wGY4 +11959 m\\Q.yt.VGw4 +11959 m[.yt.N/G4 +11962 n[.yt/G4 +11964 m[.yt/G4 +11962 l[z.yt,dG4 +11960 l[q.yt.SH 4 +5798 #"nadia"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +3121 #"SPRlogger"SPRsummary:-7.57RIU,225RIU,230RIU,316RIU,none,none,none after 2531s +2818 #"nadia"-> Power.sampler :OFF +2 .Gate.power :sampler,:OFF +217 "Can"m[C/yt$H+4 +11957 m[m/yt$HI4 +11959 l[ /yt%|KHg4 +2266 #"nadia"-> Power.sampler :ON +4 .Gate.power :sampler,:ON +9685 "Can"n[B/ysDH4 +3055 #"nadia"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +8898 "Can"m[./yt$H4 +11956 k[;/yt,QH4 +11956 m[8/yt+ H4 +10915 #"PROCESSING"SPR analysis completed +549 #"SPRlogger"Ended log spr26aug0111h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +56 "carousel0"Fq% +3 "core" %[j \ +7 "sampler0"H!% +4 "core" ȡ%# +4 ."closeHandoff"Handoff.select :close +5 "valve0"D +49 ."detached from nadia"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E & +5 "core" Š& N;\ O`  +3 ."detached from nadia"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from nadia"PinPort.seek :clear +3 "valve1"E&( +79 "core" Ŧ( +2 ."detached from nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 F% +3 "core" Ɠ%[ \ +4 ."detached from nadia"Carousel.home.seek 22 +3 "carousel0"F0' +16 "core" ư' +2 "carousel0"F>% +4 "core" ƾ%2[ 2 +2 "syringe1"C8& +4 "core" ø&? +3 ."detached from nadia"Plunge.seek :locked +4 "valve0"D5'Z +7 "core" ĵ' +2 "syringe0"B& +8 "core" ž&* +4 ."Archive"Archive.in :unlocking +4 "syringe1"C% +3 "syringe0"B? PZ-2 +7 "core" ß%݇/ +2 ."Archive"Archive.seek :clear +6 ."Air"Air.in :unlocking +6 "syringe0"B1(j +3 "syringe1"C6& +4 C> P]z-2 +8 ."Air"Air.seek :clear +4 "syringe1"Ck' +7 "core" ö&?T +4 ."Lysis1"Lysis1.in :unlocking +4 "syringe0"B?% +6 "syringe1"C? P[(2 +4 "core" ¿%Ld +3 ."Lysis1"Lysis1.seek :clear +9 ."Lysis2"Lysis2.in :unlocking +2 "syringe1"CB( +3 "syringe0"B> P\\k(2 +7 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bu'j +80 "Can"lZ/yrkH4 +303 "core" ' +9 ±( +11 ( +7 ' +3 "sampler0"H4% +4 "core" ȴ%# +10 ."detached from nadia"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +3 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785602629.82EDT @EDT1785602745.69 "Can"nZ/ytɲI4 +11953 nZ/ ytdMI94 +11954 mZ/ ytIW4 +11956 kZ/ yt0˂Iu4 +11957 mZ/\ ytI4 +11959 lZ/yt̶I4 +11960 l[!/ yt!PI4 +11960 mZ/yt"I4 +11958 lZ/ yt΅J 4 +11958 mZ/ yt J)4 +11961 mZ/ytϻJG4 +11958 nZP/ ytVJe4 +11958 jZ/yt+J4 +11955 lZ~/ytъJ4 +11954 mZ/yt %J4 +11953 kZ/yt>J4 +11950 lZ/yt"ZJ4 +11951 oZ/yt"K4 +11953 nZ/ ytԐK74 +5974 #"bill"Session Begun +659 #-> exit +3 #Session Ended +5320 "Can"m[n/yt*KU4 +11954 j[/ytKs4 +11619 #"nadia"-> Power.sampler :ON +3 .Gate.power :sampler,:ON +334 "Can"m[/yt_K4 +1078 #"nadia"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +2396 #-> ESP.configure +5 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +4 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +3 DD +2 "core"  +8 ."nadia"Plunge.configure :default +2 "valve0"D# P ޤ-2 +12 ."nadia"SamplePump.configure :default + "sampler0"H#^ #(22 +11 ."nadia"Toroid.configure :default +2 "valve0"D$ ֜2 +10 ."nadia"Carousel.configure :default + "carousel0"F#<` F 22 +9 ."nadia"Abs.configure :default +2 "ambient0"J#9 e  +9 ."nadia"PhaseSeparator.configure :full +2 "carousel1"G$ +8 ."nadia"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."nadia"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."nadia"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."nadia"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 ."nadia"PinPort.configure :default +2 "valve1"E$ B22 +6 #"nadia"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +251 #-> Power.sampler :ON +3 .Gate.power :sampler,:ON +7804 "Can"j[/ys?ۢK4 +6428 #"nadia"-> Head.psi +5 "carousel1"GE& +8 "core" &Pr +288 #"nadia"-> Head.psi +3 "carousel1"GM& +5 "core" &Or +5219 "Can"lZ/ysJ?uK4 +4101 #"nadia"-> Power.sampler :OFF +3 .Gate.power :sampler,:OFF +702 #-> ESP.reready! +6 ."Plunge_home"Plunge.home.jog 0 +6 ."Toroid_home"Toroid.home.jog 0 +6 ."Carousel_home"Carousel.home.jog 0 +10 "valve0"De) +5 DX* +6 ."Archive_home"Archive.home.jog 0 +9 "carousel0"FL) +8 ."Air_home"Air.home.jog 0 +8 ."Lysis1_home"Lysis1.home.jog 0 +6 "core" ) +5 ."Lysis2_home"Lysis2.home.jog 0 +6 ."PinPort_home"PinPort.home.jog 0 +5 "syringe0"BC* +3 "syringe1"C7) +5 "core" * +2 "valve0"DY% +2 "syringe1"CW* +4 "syringe0"BZ) +5 "valve1"EA* +2 "core" ) +3 "valve0"Df& +3 "core" * +5 "syringe0"B& +30 "core" %UG3OS  + ÷) * + ) + &X,}O  + * &}S]b +7 "valve1"E[& +6 "syringe0"B % +5 "syringe1"C_% +2 ."Toroid_home"Toroid.in :homing +5 "syringe1"C]& +4 ."Plunge_home"Plunge.in :homing +12 ."Archive_home"Archive.in :homing +2 "valve0"D?2 +4 D> (2 +2 "syringe0"B? -2 +7 ."Toroid_home"Toroid.jog 1355 +4 ."Plunge_home"Plunge.jog 1478 +7 "valve0"D*K +3 ."Archive_home"Archive.jog 1609 +5 "valve0"DG) +6 "syringe0"B^*I +9 "core" &Od  + ‰%xٷ`  + %ݬ + &x^  +6 ."Lysis2_home"Lysis2.in :homing +5 ."Lysis1_home"Lysis1.in :homing +4 ."PinPort_home"PinPort.in :homing +4 ."Air_home"Air.in :homing +8 "syringe0"B> (2 +3 "syringe1"C? (2 +3 "valve1"E?2 +2 "syringe1"C> -2 +10 ."PinPort_home"PinPort.jog 1232 +6 ."Lysis2_home"Lysis2.jog 1615 +5 ."Lysis1_home"Lysis1.jog 1595 +5 ."Air_home"Air.jog 1426 +4 "valve1"E`* +4 "syringe0"B2)O +4 "syringe1"Cb*; +3 Ci) +32 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +3 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"DT' +5 "core" * +4 ."Archive_home"Archive.setRawPosition! 50 +2 "syringe0"B82 +6 ."Archive_home"Archive.seek :home +3 "syringe0"BJ( +7 "core" ' +3 ."Plunge_home"Plunge.in :default + "valve0"D> P ޤ-2 +14 "core" ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +22 "core" ) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +8 ."Air_home"Air.seek :home +3 "syringe1"Ch' +7 "core" ²) + * + ' +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +7 ."Air_home"Air.in :default +5 ."Lysis2_home"Lysis2.setRawPosition! 50 +4 "syringe1"C82 +3 C>d P]z<2 +3 "syringe0"B72 +8 ."Lysis1_home"Lysis1.seek :home +8 ."Lysis2_home"Lysis2.seek :home +4 "syringe1"Cg( +3 "syringe0"B;' +10 "core" * +2 ."PinPort_home"PinPort.setRawPosition! 3150 + "valve1"E8 N +7 ."PinPort_home"PinPort.seek :home +5 "valve1"Ej( +7 "core" ( + »' +2 ."Lysis1_home"Lysis1.in :default +4 ."Lysis2_home"Lysis2.in :default +3 "syringe1"C?d P[<2 +4 "syringe0"B>d P\\k<2 +113 "core" Ę* +4 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +2 "valve0"Dp( +5 "core" ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +275 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +83 ."clearToroid"Toroid.seek :clear +5 "valve1"Et& +4 "valve0"DH( +9 "core" &OY q +7 ."nadia"PinPort.in :unlocking +2 "valve1"E? B2 +5 ."nadia"PinPort.seek :clear +2 "valve1"Ev( +42 "core" ( +296 ( +3 ."nadia"Air.seek :clear +3 "syringe1"Cx' +6 "core" ' +4 ."nadia"Lysis1.seek :clear +2 "syringe1"Ca( +70 "core" ( +2 ."nadia"Lysis2.seek :clear +3 "syringe0"By'j +76 "core" ' +3 ."nadia"Archive.seek :clear +2 "syringe0"BN(j +77 "core" ( +2 ."nadia"Carousel.in :default +2 "carousel0"F><` F 22 +6 FQ% +3 "core" %T[v  +4 ."nadia"Carousel.home.seek 22 +3 "carousel0"F\\' +16 "core" ' +4 "valve0"DP% +4 "core" %^LOp !j +3 "valve0"Dd% +3 "core" %^Ou !q +4 "sampler0"Hc% +5 "core" %F +3 "valve0"D@& +4 "core" &QOr a +2 "carousel0"FS% +4 "core" %Y[  +3 "ambient0"J}% +4 "core" %\ +3 "carousel1"G{& +4 "core" &Y +3 "syringe0"B& +5 "core" &jVM{ +2 "syringe1"Cl% +5 "core" %ݴM| +2 "syringe1"C& +4 "core" Á&|z +3 "syringe0"B% +5 "core" ‚%j٭I +2 "valve1"E& +4 "core" Ń&4Op  +646 #"nadia"-> ESP.shutdown +5 "sampler0"H% +3 "core" Ȅ%Q +10 ."nadia"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +9 .Gate.power :core,:OFF +2 .Gate.power :main,:OFF +2381 #-> MainThread.exit +5 #mEXIT +15 !"nadia"o:ThreadError: mesg"killed thread:@timestampu: Time 1鉇:bt["3/home/esp/esp2/lib/schedule.rb:296:in `wakeup'"8/home/esp/esp2/lib/schedule.rb:296:in `threadReady'"3/home/esp/esp2/lib/schedule.rb:304:in `unsync'"(eval):4:in `gets'"1/home/esp/esp2/lib/cmdserver.rb:443:in `run'"0/home/esp/esp2/lib/prompter.rb:19:in `call'"6/home/esp/esp2/lib/prompter.rb:19:in `callKbdPrc'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"5/home/esp/esp2/lib/rmutex.rb:99:in `synchronize'"./home/esp/esp2/lib/rmutex.rb:72:in `lock'"./home/esp/esp2/lib/delay.rb:78:in `claim'"9/home/esp/esp2/lib/prompter.rb:37:in `claimKeyboard'"1/home/esp/esp2/lib/cmdserver.rb:442:in `run'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +7 #Session Ended @1785605592.52EDT @1785606912.94EDT = =mMAIN_scheduler =tMAIN @EDT1785606914.33 #t\ Environmental Sample Processor built 17-Jul-26 (24b6d875) +150 #"core_reset"Sent BREAK +3 #t>BREAK< ESP3 Gateway v1.44 +224 #>BREAK< ESP3 Gateway v1.44 for core bus +3 #>BREAK< ESP3 Gateway v1.44 for cartridge bus +408 #\ Configuration for honu by brent@mbari.org 7/17/26\ /home/esp/esp2/type/auv/honu/configure.rb\ +76 #Sea pressure (Abs) tare is 6% of full scale +793 "Can"j`,yu; +2 j`,yu; +2 #tPolling Can every 2:00 +14 #Waiting for espclients... +40 "Can"i`,yu; +317 #"bill"Session Begun +923 #-> Cart.aspects +778 #"nadia"Session Begun +750 #-> showlog +9198 "Can"l`q,yu\ +839 #"bill"-> Cart.aspectsOf :standard, extraDilutions:[1], lysed:950, lysed2:950 +930 #-> Cart.aspects +2684 #-> slot +5979 #"nadia"-> exit +2 #Session Ended +1532 "Can"l`m,yuS( +11967 k`S,yuF +11969 k`,yund +11967 l`T,yu +11968 i`},yu +11968 j`,yu +11967 l`^,yu +11968 k`,yu 6 +11969 m`,yu  +11969 j`8,yu\ N6 +11968 j`v,yu\ T +4957 #"bill#2"Session Begun +561 #-> Cart.aspects +6449 "Can"m`L,yu fr +11966 k`2,yu  +11967 k`#,yu ~ +11967 l` ,yu \  +11964 m`,yu  +11966 l`e,yu! +11968 k`,yu& +11967 k`/,yu9D +6403 #"bill#2"Session Ended +607 #"bill"Session Ended +4957 "Can"m`P,yub +1242 #"bill"Session Begun +443 #-> slot +2194 #-> slot 60..1=>nil +472 #-> slot +646 #-> Cart.aspects +6970 "Can"l`p,yuR +11966 j`,yu +11964 k`,yui +11966 ma#,yu +2264 #"bill"-> slot 50 =>[:standard] +2254 #-> slot 50 =>[:standard, :microSPR_phyto] +7449 "Can"la.,yu +11967 ja=,yu  +11968 kaC,yu4 +11967 kat,yu"R +11965 ma,yup +11963 laT,yu9 +11963 kad,yu +11964 jaE,yuQ +8893 #"bill"Session Ended +3069 "Can"ka>,yu +11969 ka8,yuj +11966 jaM,yu$ +11966 naj,yuB +11236 #"bill"Session Begun +194 #-> slot +535 "Can"ma,yu ` +6861 #"bill"-> slot [50..43,14..12} =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +3 !o:SyntaxError: mesg"compile error\ bill:2: syntax error, unexpected '}', expecting ']'\ slot [50..43,14..12} =>[:microSPR_phyto, :standard...\ ^:@timestampu: Time 3x1:bt[" bill:2:in `topLevelBinding'" slot [50..43,14..12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +4676 #-> SC.to 47 +6 !o: BusOff : mesg"*Storage Carousel (SC) is not powered!:@timestampu: Time 3?8:@checkpoint: omitted:bt["6/home/esp/esp2/lib/slide.rb:303:in `holdIfNeeded'"7/home/esp/esp2/lib/slide.rb:381:in `unlimitedSeek'"//home/esp/esp2/lib/slide.rb:380:in `times'"7/home/esp/esp2/lib/slide.rb:380:in `unlimitedSeek'"5/home/esp/esp2/lib/thread.rb:74:in `recoverable'"7/home/esp/esp2/lib/slide.rb:378:in `unlimitedSeek'"./home/esp/esp2/lib/slide.rb:370:in `seek'"0/home/esp/esp2/lib/slide.rb:420:in `moveTo'"1/home/esp/esp2/lib/slide.rb:430:in `driveTo'",/home/esp/esp2/lib/msg.rb:68:in `retry'"1/home/esp/esp2/lib/slide.rb:429:in `driveTo'"4/home/esp/esp2/type/auv/preconfig.rb:28:in `to'" bill:4:in `topLevelBinding'" ESP.ready! +5 .Gate.power :sampler,:OFF,limit to 3500mA +4 .Gate.power :skimmer,:OFF,limit to 3500mA +3 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +19 #Powering core bus +225 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +40 "valve0"DP +4 D +3 "core" ā +7 ."bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."bill"Abs.configure :default +2 "ambient0"J#  +13 ."bill"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."bill"Archive.configure :default +3 "syringe0"B$d PZ<2 +8 ."bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."bill"Lysis1.configure :default +3 "syringe1"C$d P[<2 +6 ."bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."bill"PinPort.configure :default + "valve1"E$ B22 +7 #"bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +8 "valve0"D% +5 D& +5 "carousel0"F% +8 "syringe0"B& +3 "core" Ă%^O0 $ + ă&_O = + Ƅ%N[Q  +14 "syringe1"C% +6 ."Toroid_home"Toroid.home.jog 0 +8 "syringe1"C& +4 ."Carousel_home"Carousel.home.jog 0 +8 ."Plunge_home"Plunge.home.jog 0 +5 "syringe0"B% +3 "valve1"E & +2 "valve0"D\ * +5 "carousel0"F ) + "valve0"D ) +23 "core" …&Xf  + Æ%ݶd Ç&فb! 3 + ˆ%ٻ]  + ʼn&+O( ,\\ + Ċ* Č) + Ƌ) +9 ."PinPort_home"PinPort.home.jog 0 +14 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +4 "valve0"D % +2 ."Lysis1_home"Lysis1.home.jog 0 +6 "valve0"D& +4 "valve1"E* +3 ."Archive_home"Archive.home.jog 0 +9 "syringe0"B) +51 "syringe1"C) +6 C* +4 "syringe0"B* +9 "core" č%^O- + Ď&a,O' + ŏ* + ) + Ñ) +3 "valve1"E& +6 "syringe0"B% +6 "syringe1"C% + ."Plunge_home"Plunge.in :homing +11 ."Toroid_home"Toroid.in :homing +4 "valve0"D> (2 +3 D?2 +7 ."Plunge_home"Plunge.jog 434 +3 ."Toroid_home"Toroid.jog 1356 +5 "valve0"D) +2 D*L +11 "core" Ò* + “* + Ŕ&.O$  + •%xٵg  Ö%\ ݫ[  +4 "syringe1"C& +10 "syringe0"B& +4 ."Lysis2_home"Lysis2.in :homing +8 ."Air_home"Air.in :homing +4 ."PinPort_home"PinPort.in :homing +5 "syringe0"B> (2 +3 "syringe1"C> -2 +2 "valve1"E?2 +6 ."Air_home"Air.jog 1426 +6 ."Lysis2_home"Lysis2.jog 1615 +5 "syringe1"C) +2 ."PinPort_home"PinPort.jog 1227 +8 "syringe0"B)O +2 "valve1"E* +9 "core" Ù&nZ  + š&SZ  + ė) +4 ."Plunge_home"Plunge.setRawPosition! 50 +5 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +4 "valve0"D72 +4 "syringe1"C? (2 +2 "syringe0"B? -2 +10 ."Plunge_home"Plunge.seek :home +3 ."Lysis1_home"Lysis1.jog 1597 +6 ."Archive_home"Archive.jog 1609 +4 "valve0"D' +3 "syringe1"C*= +5 "syringe0"B *I +19 "core" Ğ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +16 "core" Û) +3 ."Air_home"Air.setRawPosition! 0 + "syringe1"C7 +6 ."Air_home"Air.seek :home +3 "syringe1"C!' +9 "core" á' + œ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Air_home"Air.in :default +4 "syringe0"B72 +2 "syringe1"C>d P]z<2 +8 ."Lysis2_home"Lysis2.seek :home +6 "syringe0"B"' +23 "core" ŝ* +2 ."PinPort_home"PinPort.setRawPosition! 3150 + "valve1"E8 N +6 "core" ¢' +4 ."Lysis2_home"Lysis2.in :default +5 ."PinPort_home"PinPort.seek :home +4 "syringe0"B>d P\\k<2 +3 "valve1"E#( +10 "core" ß* +  * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Archive_home"Archive.setRawPosition! 50 +5 "syringe1"C82 +2 "syringe0"B82 +8 ."Lysis1_home"Lysis1.seek :home +4 ."Archive_home"Archive.seek :home +5 "syringe1"C$( +4 "syringe0"B%( +23 "core" ä( +4 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +4 "core" ¥( +3 ."Archive_home"Archive.in :default +3 "syringe0"B?d PZ<2 +81 "core" Ę* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +5 ."Toroid_home"Toroid.seek :home +3 "valve0"D&( +6 "core" Ħ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +250 "core" ţ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +34 ."clearToroid"Toroid.seek :clear +4 "valve1"E'& +2 "valve0"D(( +6 "core" ŧ&´O% L +6 ."bill"PinPort.in :unlocking +3 "valve1"E? B2 +6 ."bill"PinPort.seek :clear +3 "valve1"E)( +42 "core" ũ( +294 Ĩ( +2 ."bill"Air.seek :clear +3 "syringe1"C*' +8 "core" ê' +2 ."bill"Lysis1.seek :clear +3 "syringe1"C+( +69 "core" ë( +2 ."bill"Lysis2.seek :clear +3 "syringe0"B,'j +76 "core" ¬' +4 ."bill"Archive.seek :clear +2 "syringe0"B-(j +77 "core" ­( +2 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F.% +3 "core" Ʈ%S[MP  +4 ."bill"Carousel.home.seek 22 +2 "carousel0"F/' +19 "core" Ư' +2 "valve0"D0% +4 "core" İ%^O< !M +3 "valve0"D1% +4 "core" ı%^O7 !Z +4 "sampler0"H2% +8 "core" Ȳ%; +3 "valve0"D3& +5 "core" ij&]O< U +2 "carousel0"F4% +5 "core" ƴ%N[Q  +2 "ambient0"J5% +6 "core" ʵ%( +2 "carousel1"G6& +4 "core" Ƕ&MuQ\ +3 "syringe0"B7& +4 "core" ·&j*T +3 "syringe1"C8% +4 "core" ø%ݨT +3 "syringe1"C9& +4 "core" ù&IW +3 "syringe0"B:% +4 "core" º%j٢! +3 "valve1"E;& +4 "core" Ż&MO7  +2275 #"bill"-> SC.to 47 +4 .Carousel.seek 47 +3 "carousel0"F<', +477 "Can"laf,yu$ +55 "core" Ƽ' +3 "carousel0"F=% +3 "core" ƽ%,(ƇS 2 +1040 #"bill"-> SC.to 55 +4 .Carousel.seek 55 +4 "carousel0"F>'4 +187 "core" ƾ' +2 "carousel0"F?% +4 "core" ƿ%41T 2 +3434 #"bill"-> SC.to 49 +4 .Carousel.seek 49 +4 "carousel0"F@'. +146 "core" ' +3 "carousel0"FA% +42 "core" %.W"QV  +742 #"bill"-> SC.to 60 +5 .Carousel.seek 60 +3 "carousel0"FB'9 +247 "core" ' +2 "carousel0"FC% +4 "core" %9(S 2 +1219 #"bill"-> SC.to 53 +5 .Carousel.seek 53 +4 "carousel0"FD'2 +166 "core" ' +2 "carousel0"FE% +4 "core" %2)S 2 +1161 #"bill"-> SC.to 43 +5 .Carousel.seek 43 +2 "carousel0"FF') +227 "core" ' +2 "carousel0"FG% +4 "core" %) ,|T 2 +699 #"bill"-> SC.to 45 +3 .Carousel.seek 45 +5 "carousel0"FH'+ +66 "core" ' +2 "carousel0"FI% +4 "core" %+4pS 2 +2445 "Can"ma,yuH +11966 ja,yuH! +6001 #"bill"-> Cart.standard +5964 "Can"l`,yuI$ +11966 m`,yuJ' +11966 ka,yuK*2 +1680 #"bill"-> slots 1..60=>nil +400 #-> slots +9887 "Can"ja!,yuM-P +3950 #"bill"-> slot[50,48,46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +11 !o:SyntaxError: mesg"compile error\ bill:16: syntax error, unexpected tASSOC, expecting $end\ ...46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {...\ ^:@timestampu: Time 33n:bt["!bill:16:in `topLevelBinding'" slot [50,48,46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] +1421 #-> Cart.aspects +1714 #-> Cart.standard +1409 #-> SC.to 50 +3 .Carousel.seek 50 +4 "carousel0"FJ'/ +126 "core" ' +2 "carousel0"FK% +4 "core" %/(GT 2 +938 "Can"la-,yuM&0n +9443 #"bill"-> SC.to 48 +5 .Carousel.seek 48 +2 "carousel0"FL'- +65 "core" ' +3 "carousel0"FM% +4 "core" %-6TG 2 +2449 "Can"kaA,yuI3 +3797 #"bill"-> SC.to 46 +4 .Carousel.seek 46 +4 "carousel0"FN'+ +63 "core" ' +2 "carousel0"FO% +4 "core" %+8[TL 2 +4593 #"bill"-> SC.to 44 +5 .Carousel.seek 44 +3 "carousel0"FP'* +66 "core" ' +2 "carousel0"FQ% +4 "core" %*)T 2 +3423 "Can"laJ,}yuM+6 +689 #"bill"-> SC.to 26 +5 .Carousel.seek 26 +2 "carousel0"FR'u +391 "core" ' +2 "carousel0"FS% +5 "core" %u6l{T4 2 +4333 #"bill"-> SC.to 24 +4 .Carousel.seek 24 +4 "carousel0"FT' +65 "core" ' +2 "carousel0"FU% +4 "core" % SC.to 22 +5 .Carousel.seek 22 +4 "carousel0"FV' +64 "core" ' +2 "carousel0"FW% +4 "core" %:[T4 2 +1140 "Can"ha2,~yuK9 +2596 #"bill"-> SC.to 20 +5 .Carousel.seek 20 +2 "carousel0"FX' +64 "core" ' +2 "carousel0"FY% +4 "core" %:S`TR 2 +4792 #"bill"-> SC.to 14 +5 .Carousel.seek 14 +3 "carousel0"FZ' +146 "core" ' +2 "carousel0"F[% +5 "core" % <9TZ 2 +4343 "Can"la8,yuJ< +515 #"bill"-> SC.to 12 +4 .Carousel.seek 12 +4 "carousel0"F\\'\ +65 "core" ' +2 "carousel0"F]% +4 "core" %\ 91\ TR 2 +11375 "Can"kaH,yuK? +1701 #"bill"-> SC.to 12 +5 .Carousel.seek 12 +2 "carousel0"F^'\ +16 "core" ' +2 "carousel0"F_% +5 "core" %\ %1T 2 +7996 #"bill"-> to 12 +3 "valve0"D`% +5 "core" %^O< !O +3 "syringe1"Ca& +4 "core" &[j +2 "syringe1"Cb% +4 "core" %ݷ +3 "valve1"Ec& +4 "core" &,O8  +4 ."bill"Carousel.seek 12 +5 "carousel0"Fd'\ +19 "core" ' +4 "carousel0"Fe% +5 "core" %\ 51T7 2 +2 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"Cf& +3 "core" &bg +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."bill"Lysis1.seek :locked +3 "syringe1"Cg( +249 "core" ( +3 "valve0"Dh% +6 "core" %^O !R +3 ."Plunge"Plunge.seek :locked +3 "valve0"Di'Z +6 "syringe1"Cj% +7 "core" %ݶau +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"Ck'6 +80 "core" ' +23 ' +3 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 Fl% +3 "core" %\ [1_Q#  +7 #"bill"Powering cartridge bus +264 "cartridge_broadcast" ( +2 ."bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" mt +3 "cartridge" t +3 "Cartridge" ny +4 "cartridge" y4646 +4 "Cartridge" oy  +3 "cartridge" y :L+0J59912 N+0ŊCxޜ +4 #"bill"Mounted lysis Cartridge board#J59912 +9 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +405 #-> to 13 +2 "carousel0"Fp% +6 "core" %\ 1Pi \ +3 "valve0"Dq% +45 "core" %ZG6O& & +4 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"Er& +4 "core" &O&  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 Fs% +3 "core" %\ 1_P \ +4 ."bill"Carousel.home.seek 12 +2 "carousel0"Ft'\ +17 "core" ' +3 "carousel0"Fu% +4 "core" %\ W1QS  +3 "syringe0"Bv& +4 "core" &jf +3 "syringe1"Cw% +4 "core" %6Pb  +15 ."Air"Air.in :unlocking +4 "syringe0"Bx% +3 "syringe1"C> P]z-2 +6 "core" %j٧_ +3 ."Air"Air.seek :clear +3 "syringe1"Cy' +96 "core" ' +2 "syringe1"Cz& +4 "core" & +5 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +5 "valve0"D{% +6 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C|( +6 "core" %ZG?OM  +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +4 "valve0"D}'8 +96 "core" ' +10 ( +3 ."bill"Carousel.seek 13 +5 "carousel0"F~' +43 "core" ' +4 "carousel0"F% +3 "core" % 75KTK 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C& +4 "core" Á&TT +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +2 "syringe1"C( +250 "core" Ä( +2 "valve0"D% +8 "core" ă%8[O +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +6 ."Plunge"Plunge.seek :locked +2 "valve0"D'Z +7 "syringe1"C % +11 "core" É%ݨR +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +4 "syringe1"C'6 +64 "core" Ă' +40 È' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F% +3 "core" Ƈ% Z5LQ/  +6 #"bill"Powering cartridge bus +64 "Can"ka,yuTB" +203 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +6 ."bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y +4 "cartridge" yAA +5 "Cartridge" y  +5 "cartridge" y a"&0J59949 Y%&0lC/q +3 #"bill"Mounted lysis Cartridge board#J59949 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1132 #-> to 14 +3 "carousel0"F % +5 "core" ƍ% 5LPr \  +3 "valve0"D% +4 "core" Ď%ZG5O+ ' +5 ."bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +3 "valve1"E& +6 "core" Ŕ& O'  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F% +3 "core" ƕ% 5LOPg \  +3 ."bill"Carousel.home.seek 13 +4 "carousel0"F' +17 "core" Ɩ' +2 "carousel0"F% +5 "core" ƙ% 65ST, 2 +2 "syringe0"B& +4 "core" š&j"Z +3 "syringe1"C % +4 "core" Ì%6ځ ` +4 ."Air"Air.in :unlocking +4 "syringe0"B\ % +3 "syringe1"C> P]z-2 +7 "core" Š%j٧N +4 ."Air"Air.seek :clear +2 "syringe1"C' +95 "core" Ñ' +3 "syringe1"C& +4 "core" Ð& +4 ."Lysis1"Lysis1.in :unlocking +5 "valve0"D% +3 "syringe1"C? P[(2 +7 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C( +5 "core" ď%ZG9OG # +5 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"D'8 +97 "core" Ē' +12 ×( +3 ."bill"Carousel.seek 14 +3 "carousel0"F' +47 "core" Ɠ' +4 "carousel0"F% +3 "core" ƛ% 89TL 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C& +3 "core" Ü&VE +4 ."bill"Lysis1.in :fast + "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +2 "syringe1"C( +250 "core" Ý( +3 "valve0"D% +8 "core" ğ%8[O +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +4 "syringe1"C % +7 "valve0"D''Z +4 "core" à%ݘ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C'6 +76 "core" ħ' +28 Ø' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F&% +3 "core" Ʀ% [9Q9  +46 #"bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +16 ."bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +8 ."bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" #y +5 "cartridge" yGBGB +3 "Cartridge" .y  +6 "cartridge" y _S&0J59913 U&0(CJ +3 #"bill"Mounted lysis Cartridge board#J59913 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +741 #-> to 20 +5 "carousel0"F0% +6 "core" ư% 9P~ \ +3 "valve0"D1% +4 "core" ı%ZGKO5  +5 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +5 "valve1"E2& +3 "core" Ų&/O<  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F!% +3 "core" ơ% 9OPv \ +4 ."bill"Carousel.home.seek 14 +4 "carousel0"F3' +15 "core" Ƴ' +3 "carousel0"F$% +4 "core" Ƥ% %9T 2 +3 "syringe0"B4& +5 "core" ´&j! +2 "syringe1"C5% +5 "core" õ%6 +4 ."Air"Air.in :unlocking +4 "syringe0"B6% +4 "syringe1"C> P]z-2 +6 "core" ¶%j٢ +3 ."Air"Air.seek :clear +5 "syringe1"C7' +95 "core" ÷' +3 "syringe1"C8& +5 "core" ø& +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"D9% +5 "syringe1"C? P[(2 +7 "core" Ĺ%ZGCO3  +3 ."Lysis1"Lysis1.seek :clear +6 "syringe1"C:( +4 ."Plunge"Plunge.in :unlocking +5 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +2 "valve0"D;'8 +95 "core" Ļ' +20 ú( +3 ."bill"Carousel.seek 20 +3 "carousel0"F"' +148 "core" Ƣ' +6 "carousel0"F%% +3 "core" ƥ%&S]T 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C & +3 "core" Ë&R +4 ."bill"Lysis1.in :fast + "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +3 "syringe1"C/( +249 "core" ï( +3 "valve0"D=% +6 "core" Ľ%8[oO +4 ."Plunge"Plunge.in :default + "valve0"D> P ޤ-2 +6 "syringe1"C?% +5 ."Plunge"Plunge.seek :locked +5 "valve0"D<'Z +4 "core" ÿ%ݜ +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CA'6 +77 "core" ļ' +28 ' +3 ."bill"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 F>% +3 "core" ƾ%RSVMQ  +7 #"bill"Powering cartridge bus +266 "cartridge_broadcast" ( +2 ."bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" Cy +4 "cartridge" yՎՎ +4 "Cartridge" @y  +3 "cartridge" y /J59971 Z/MC% +4 #"bill"Mounted lysis Cartridge board#J59971 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1107 #-> to 22 +2 "carousel0"FE% +5 "core" %SVP \ +3 "valve0"DB% +4 "core" %ZG7OA % +5 ."bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +3 "valve1"EG& +5 "core" &)OA  +2 ."bill"Carousel.in :default +3 "carousel0"F><` F 22 +6 FD% +4 "core" %SU}P \ \ +3 ."bill"Carousel.home.seek 20 +4 "carousel0"FI' +18 "core" ' +2 "carousel0"FF% +4 "core" %5S`T 2 +3 "syringe0"BK& +4 "core" &j +3 "syringe1"CH% +5 "core" %6&r , +4 ."Air"Air.in :unlocking +4 "syringe0"BM% +3 "syringe1"C> P]z-2 +6 ."Air"Air.seek :clear +3 "syringe1"CJ' +4 "core" %j٧s +90 ' +3 "syringe1"CO& +4 "core" & +4 ."Lysis1"Lysis1.in :unlocking +4 "valve0"DL% +4 "syringe1"C? P[(2 +6 "core" %ZG=O4  +2 ."Lysis1"Lysis1.seek :clear +7 "syringe1"CQ( +4 ."Plunge"Plunge.in :unlocking +5 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"DN'8 +96 "core" ' +19 ( +3 ."bill"Carousel.seek 22 +4 "carousel0"FP' +62 "core" ' +5 "carousel0"FS% +3 "core" %)[T 2 +3 ."bill"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"CU& +3 "core" &\\j +4 ."bill"Lysis1.in :fast + "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +3 "syringe1"CR( +249 "core" ( +13 "valve0"DW% +8 "core" %8[O- +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 "syringe1"CT% +5 ."Plunge"Plunge.seek :locked +7 "valve0"DY'Z +8 "core" %ݙn +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CV'6 +77 "core" ' +28 ' +3 ."bill"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 F[% +3 "core" %R[Q  +6 #"bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" Xy +4 "cartridge" y&:&: +3 "Cartridge" ]y  +5 "cartridge" y @?+0J59951 A+0C@ +4 #"bill"Mounted lysis Cartridge board#J59951 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +593 #-> to 24 +2 "carousel0"FZ% +5 "core" %[Pu \ +3 "valve0"D_% +5 "core" %ZGIO>  +4 ."bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +3 "valve1"E\\& +5 "core" &&OA  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +5 F`% +3 "core" %[NPy \ +4 ."bill"Carousel.home.seek 22 +3 "carousel0"Fa' +18 "core" ' +2 "carousel0"Fb% +5 "core" %)[T 2 +3 "syringe0"Bc& +4 "core" &jo +3 "syringe1"Ce% +5 "core" %6g +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +5 "syringe0"Bf% +6 ."Air"Air.seek :clear +4 "syringe1"C^' +4 "core" %j٦s +90 ' +2 "syringe1"Ch& +5 "core" & +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +5 "valve0"Dj% +8 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C+( +5 "core" %ZGWO<  +5 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +4 "valve0"Dl'8 +96 "core" ' +11 ë( +3 ."bill"Carousel.seek 24 +5 "carousel0"F' +63 "core" ƞ' +5 "carousel0"F*% +3 "core" ƪ%+dZT 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"Cm& +3 "core" &S\\ +4 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."bill"Lysis1.seek :locked +2 "syringe1"Cn( +249 "core" ( +3 "valve0"Do% +10 "core" %8[4O +4 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 "syringe1"Cp% +5 ."Plunge"Plunge.seek :locked +5 "valve0"Dq'Z +6 "core" %ݚ[ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +2 "syringe1"Cr'6 +77 "core" ' +28 ' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 Fs% +3 "core" %QdTQ  +8 #"bill"Powering cartridge bus +266 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +15 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" uy +5 "cartridge" y RI +4 "Cartridge" vy  +4 "cartridge" y k`/ C;R c/J59986 +5 #"bill"Mounted lysis Cartridge board#J59986 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +476 #-> to 26 +2 "carousel0"Fw% +5 "core" %dTP \  +3 "valve0"Dx% +5 "core" %ZGHO,  +5 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +8 "valve1"Ez& +6 "core" &"O-  +2 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F{% +3 "core" %dSP \  +4 ."bill"Carousel.home.seek 24 +3 "carousel0"Fd' +16 "core" ' +2 "carousel0"Fk% +5 "core" %(dZT\ 2 +3 "syringe0"B& +5 "core" &j^ +2 "syringe1"C% +5 "core" Á%6Q h +4 ."Air"Air.in :unlocking +3 "syringe0"Bg% +4 "syringe1"C> P]z-2 +8 "core" %j٠U +3 ."Air"Air.seek :clear +3 "syringe1"Ci' +96 "core" ' +2 "syringe1"Ct& +5 "core" & +8 "valve0"D% +6 ."Lysis1"Lysis1.in :unlocking + "syringe1"C? P[(2 +6 "core" ă%ZGLO-  +3 ."Lysis1"Lysis1.seek :clear +6 "syringe1"C ( +4 ."Plunge"Plunge.in :unlocking +53 "valve0"D> P ޤ(2 +9 ."Plunge"Plunge.seek :clear +3 "valve0"D|'8 +97 "core" ' +20 É( +3 ."bill"Carousel.seek 26 +3 "carousel0"F'u +65 "core" Ƈ' +5 "carousel0"F}% +3 "core" %u4lT@ 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"Cy& +5 "core" &UJ +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 "core" Ƈ' xXl!Q^  +3 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 26 + 3 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi^Q:@pastThresholdF: @voltagef15.01427000490918!: @currenti]: @type2F:@outPressi!:\ @lostF: @inPressil:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positionix: @pastRLSF: @tagi : @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +3 Ƈ' +2 ."bill"Lysis1.seek :locked +4 "syringe1"C( +250 "core" Æ( +3 "valve0"D% +8 "core" ą%8[O +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +9 ."Plunge"Plunge.seek :locked +3 "valve0"D 'Z +5 "syringe1"C% +8 "core" Î%ݐC5 +4 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"C'6 +69 "core" č' +36 Ô' +3 ."bill"Carousel.reconfigure :locked + "carousel0"F#` F 2\ 2 +7 F% +2 "core" ƕ%uQlhQ  +8 #"bill"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y +5 "cartridge" yִjִj +4 "Cartridge" y  +5 "cartridge" y r/J59943 r/6Cr +3 #"bill"Mounted lysis Cartridge board#J59943 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1293 "Can"j`,yuQyF@ +3222 #"bill"-> to 44 +6 "carousel0"F % +5 "core" ƌ%ulP \  +3 "valve0"D\ % +6 "core" Ċ%ZGJ0OI  +4 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E& +5 "core" Ő&0 OI  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F% +3 "core" Ə%ulP \  +4 ."bill"Carousel.home.seek 26 +4 "carousel0"F~'u +15 "core" ' +2 "carousel0"F% +4 "core" ƈ%u1lT/ 2 +3 "syringe0"B& +4 "core" ›&jd +4 "syringe1"C% +3 "core" Ü%6)T ) +5 ."Air"Air.in :unlocking +4 "syringe0"B% +2 "syringe1"C> P]z-2 +8 "core" „%jٮ^ +3 ."Air"Air.seek :clear +4 "syringe1"C' +94 "core" Â' +3 "syringe1"C& +4 "core" Ö& +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"D% +4 "syringe1"C? P[(2 +6 "core" ğ%ZGWOC  +4 ."Lysis1"Lysis1.seek :clear +6 "syringe1"C ( +4 ."Plunge"Plunge.in :unlocking +4 "valve0"D> P ޤ(2 +6 ."Plunge"Plunge.seek :clear +3 "valve0"D'8 +97 "core" ė' +18 à( +3 ."bill"Carousel.seek 44 +3 "carousel0"F&'* +392 "core" Ʀ' +4 "carousel0"F% +4 "core" ƒ%*5T; 2 +3 ."bill"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"C& +5 "core" Ñ&RL +5 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 "core" Ʀ' * W Q1  +3 !o:Slide::Warning : mesg"IStorage Carousel (SC) reseek at 44 + 3 counts (sensed 44 + 4 counts): @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressi1Q:@pastThresholdF: @voltagef15.019179185076093T: @currenti\\: @type2F:@outPressi :\ @lostF: @inPressiֹ:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni *: @pastRLSF: @tagi+: @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + Ʀ' +2 ."bill"Lysis1.seek :locked +5 "syringe1"C#( +249 "core" ã( +2 "valve0"D.% +8 "core" Į%8[ O +3 ."Plunge"Plunge.in :default + "valve0"D> P ޤ-2 +6 "syringe1"C0% +5 ."Plunge"Plunge.seek :locked +5 "valve0"D1'Z +5 "core" ð%ݓJ +3 ."Air"Air.in :fast +3 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C2'6 +78 "core" ı' +26 ò' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F!% +3 "core" ơ%*QQ  +7 #"bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +6 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" $y +6 "cartridge" ym5m5 +4 "Cartridge" 4y  +4 "cartridge" y hr/J59915 r/C\\ +4 #"bill"Mounted lysis Cartridge board#J59915 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +547 #-> to 46 +3 "carousel0"F5% +5 "core" Ƶ%*P \  +3 "valve0"D6% +5 "core" Ķ%ZGcO, +5 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E8& +4 "core" Ÿ&)O-  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F9% +3 "core" ƹ%*Pi \  +4 ."bill"Carousel.home.seek 44 +3 "carousel0"F'* +18 "core" Ɠ' +45 "carousel0"F% +4 "core" Ƙ%*XQK  +3 "syringe0"B%& +5 "core" ¥&j +2 "syringe1"C% +4 "core" Ý%6 +15 "syringe0"B % +3 ."Air"Air.in :unlocking +4 "syringe1"C> P]z-2 +5 "core" ‹%j٫ +3 ."Air"Air.seek :clear +4 "syringe1"C'' +94 "core" ç' +2 "syringe1"C3& +4 "core" ó&؀ +5 ."Lysis1"Lysis1.in :unlocking +4 "valve0"D=% +3 "syringe1"C? P[(2 +11 ."Lysis1"Lysis1.seek :clear +4 "syringe1"C?( +6 "core" Ľ%ZG\\ OJ +4 ."Plunge"Plunge.in :unlocking +3 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +2 "valve0"D:'8 +97 "core" ĺ' +11 ÿ( +3 ."bill"Carousel.seek 46 +3 "carousel0"F>'+ +65 "core" ƾ' +5 "carousel0"F;% +3 "core" ƻ%+*KT 2 +5 ."bill"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"C7& +3 "core" ÷&] +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +2 "syringe1"CC( +249 "core" ( +2 "valve0"D@% +8 "core" %8[!O +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +4 "syringe1"CE% +3 "valve0"DB'Z +9 "core" %ݠ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"CG'6 +77 "core" ' +27 ' +3 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 FD% +3 "core" %+RGQ  +7 #"bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +2 "Cartridge" Fy +8 "cartridge" y|(g +4 "Cartridge" Ky ' +5 "cartridge" y /J59919 Ǔ/+i F L/C0CǍ +3 #"bill"Mounted lysis Cartridge board#J59919 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +552 #-> to 48 +3 "carousel0"FH% +5 "core" %+GnPx \  +3 "valve0"DM% +4 "core" %ZGhO9 +5 ."bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +3 "valve1"EO& +6 "core" &.O8  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 FL% +4 "core" %+G@Pf \  +7 ."bill"Carousel.home.seek 46 +4 "carousel0"F"'+ +16 "core" Ƣ' +3 "carousel0"FA% +4 "core" %+&MyT 2 +3 "syringe0"BS& +5 "core" &j +2 "syringe1"CU% +4 "core" %6 +5 ."Air"Air.in :unlocking +4 "syringe0"B/% +3 "syringe1"C> P]z-2 +8 ."Air"Air.seek :clear +3 "syringe1"C<' +4 "core" ¯%j٫ +90 ü' +2 "syringe1"CI& +4 "core" & +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"DW% +4 "syringe1"C? P[(2 +6 "core" %ZGkO9 +4 ."Lysis1"Lysis1.seek :clear +4 "syringe1"CT( +8 ."Plunge"Plunge.in :unlocking +6 "valve0"D> P ޤ(2 +7 ."Plunge"Plunge.seek :clear +3 "valve0"DQ'8 +97 "core" ' +17 ( +3 ."bill"Carousel.seek 48 +3 "carousel0"F['- +66 "core" ' +4 "carousel0"FN% +3 "core" %-(ʺT\ 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"CJ& +3 "core" &V~ +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."bill"Lysis1.seek :locked +2 "syringe1"CX( +250 "core" ( +3 "valve0"D]% +7 "core" %8[O +3 ."Plunge"Plunge.in :default + "valve0"D> P ޤ-2 +6 "syringe1"CZ% +4 ."Plunge"Plunge.seek :locked +7 "valve0"D_'Z +6 "core" %ݠ +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"C\\'6 +77 "core" ' +28 ' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F`% +3 "core" %-QʹEP  +8 #"bill"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."bill"Gate.power :heat,:ON,limit to 5000mA +47 "Cartridge" by +6 "cartridge" yrr +4 "Cartridge" cy  +5 "cartridge" y . &0J59984 u &0CCu +4 #"bill"Mounted lysis Cartridge board#J59984 +4 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +496 #-> to 50 +2 "carousel0"Fe% +5 "core" %-ʺsPp \ +3 "valve0"Df% +6 "core" %ZG`OJ +4 ."bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +3 "valve1"Eh& +5 "core" &0OK  +2 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fj% +3 "core" %-ʺNP \ +3 ."bill"Carousel.home.seek 48 +3 "carousel0"FP'- +18 "core" ' +4 "carousel0"FV% +4 "core" %-NʹQ:  +3 "syringe0"B*& +5 "core" ª&j t +2 "syringe1"Cm% +4 "core" %6r +13 ."Air"Air.in :unlocking +4 "syringe0"BR% +3 "syringe1"C> P]z-2 +8 "core" %j٪n +3 ."Air"Air.seek :clear +3 "syringe1"CY' +96 "core" ' +3 "syringe1"Ca& +4 "core" &  +5 ."Lysis1"Lysis1.in :unlocking +3 "valve0"Do% +4 "syringe1"C? P[(2 +7 "core" %ZGXOA  +3 ."Lysis1"Lysis1.seek :clear +8 ."Plunge"Plunge.in :unlocking +3 "syringe1"Cp( +2 "valve0"D> P ޤ(2 +13 ."Plunge"Plunge.seek :clear +2 "valve0"D+'8 +97 "core" ī' +17 ( +3 ."bill"Carousel.seek 50 +3 "carousel0"Fs'/ +65 "core" ' +7 "carousel0"Fl% +4 "core" %/*FT 2 +3 ."bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C^& +3 "core" &Pk +3 ."bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."bill"Lysis1.seek :locked +3 "syringe1"Cu( +250 "core" ( +2 "valve0"Dv% +8 "core" %8[O +2 ."Plunge"Plunge.in :default +2 "valve0"D> P ޤ-2 +7 ."Plunge"Plunge.seek :locked +6 "syringe1"Cw% +3 "valve0"Dx'Z +9 "core" %ݛm +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"Cz'6 +76 "core" ' +28 ' +2 ."bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F{% +3 "core" %/SB|Q  +8 #"bill"Powering cartridge bus +263 "cartridge_broadcast" ( +3 ."bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +3 "cartridge_broadcast"~ +6 ."bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +6 ."bill"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" ky +3 "cartridge" yxx +4 "Cartridge" y  +4 "cartridge" y {s/J59963 ;v/ECP +4 #"bill"Mounted lysis Cartridge board#J59963 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +1534 #-> Cartridge.unlock +5 .Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E& +5 "core" Ł&-O?  +3 ."bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 Fg% +4 "core" %/BbP \ +3 ."bill"Carousel.home.seek 50 +3 "carousel0"Ft'/ +18 "core" ' +2 "carousel0"F% +5 "core" ƃ%/NCQ9  +2 "syringe0"B& +5 "core" ž&j#Z +2 "syringe1"Cr% +4 "core" %6Z ~ +6 ."Air"Air.in :unlocking +6 "syringe0"B}% +4 "syringe1"C> P]z-2 +8 "core" %jٰW +3 ."Air"Air.seek :clear +3 "syringe1"Cy' +95 "core" ' +2 "syringe1"Cn& +4 "core" & +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +5 "valve0"Dq% +7 ."Lysis1"Lysis1.seek :clear +3 "Can"l`o,yuJJ^ +3 "syringe1"Cd( +4 "core" %ZGjO= +6 ."Plunge"Plunge.in :unlocking +2 "valve0"D> P ޤ(2 +5 ."Plunge"Plunge.seek :clear +4 "valve0"D'8 +96 "core" ą' +13 ( +4167 #"bill"-> Cart.aspets +5 !o:NoMethodError\ : mesg",undefined method `aspets' for Cartridge:@timestampu: Time 3f: name0: args0:bt["G/home/esp/esp2/utils/cartridge/carousel.rb:513:in `method_missing'"!bill:43:in `topLevelBinding'" Cart.aspects +7262 "Can"j`3,yuN2M| +11966 l`,yuN5P +11970 l_,yuLES +7963 #"bill"-> start {fireAll "standard"} +52 #"detached from bill"Cartridge group: standard\ \ +7 "valve0"D% +6 "core" Ď%8[8O( +2 "valve0"D & +5 "carousel0"F% +6 "syringe0"B|& +5 "syringe1"Ci% +59 C& +15 "syringe0"B% +7 "valve1"E & +13 "core" ĉ&O+ ( + ƕ%/OFQJ  + &j-C + %ݙ\ + Ù&] + š%j٢4 + Ō&#O#  +15 ."clearToroid"Toroid.seek :clear +4 "valve1"E\ & +5 "valve0"D( +7 "core" Ŋ&(O(  + Đ( +4 ."detached from bill"Air.in :default +2 "syringe1"C>d P]z<2 +7 ."detached from bill"Air.seek :clear +3 "syringe1"C' +5 "core" Ï' +3 ."detached from bill"Lysis1.in :default +3 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C( +4 "core" È( +5 ."detached from bill"Lysis2.seek :clear +2 "syringe0"B'j +9 "core" ›' +3 ."detached from bill"Archive.seek :clear +4 "syringe0"B(j +7 "core" œ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +7 F% +4 "core" Ƅ%/OEsQ>  +5 ."detached from bill"Carousel.home.seek 50 +4 "carousel0"F'/ +16 "core" Ɩ' +2 "valve0"D% +5 "core" ğ%8[/O" +4 #"detached from bill"Selecting Cartridge 50 +2 "valve0"D% +9 "core" ć%8[;O!  +2 "syringe1"C& +5 "core" Ô&SF +2 "syringe1"C% +5 "core" Æ%ݔI +3 "valve1"E & +5 "core" ō&OE  +3 ."detached from bill"Carousel.seek 50 +3 "carousel0"F~'/ +16 "core" ' +5 "carousel0"F% +4 "core" ƒ%/,FS 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C& +4 "core" Ñ&MF +4 ."detached from bill"Lysis1.in :fast +3 "syringe1"C?d P[<2 +7 ."detached from bill"Lysis1.seek :locked +4 "syringe1"C.( +249 "core" î( +3 "valve0"D0% +17 "core" İ%8[%O +5 ."Plunge"Plunge.in :default +3 "valve0"D> P ޤ-2 +12 "syringe1"C % +9 ."Plunge"Plunge.seek :locked +10 "valve0"D!'Z +8 "core" à%݌ +6 ."Air"Air.in :fast +4 "syringe1"C> P]z<2 +9 ."Air"Air.seek :locked +10 "syringe1"C'6 +61 "core" ġ' +69 #"preston"Session Begun +12 "core" ×' +4 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +8 F% +3 "core" Ƃ%/OBQB  +8 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +5 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +9 ."detached from bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" $y +7 "cartridge" yxx +4 "Cartridge" 4y  +5 "cartridge" y {s/J59963 ;v/ECP +3 #"detached from bill"Mounted lysis Cartridge board#J59963 +8 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +8 "valve0"D5% +4 "core" ĵ%ZGOD +2 "syringe1"C6& +6 "core" ö& v +3 "syringe1"C8% +4 "core" ø%6w +3 "carousel0"F9% +5 "core" ƹ%/BP \ +4 "carousel0"F% +4 "core" Ƙ%/BP \ +119 ."PROCESSING"SPR.command "read_sequences" +78 .SPR.command "stats_on" +11 "carousel0"F%% +3 "core" ƥ%/AP \ +5 ."PROCESSING"SPR.command "start 0 1" +72 ."SPRlogger"SPR.start /var/log/esp/spr26aug0115h.spr.bz2 +195 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +6 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +3 "syringe1"C% +8 "core" Ý%6y +42 "syringe1"C % +53 "core" Ë%6t  +3 "syringe1"C3% +6 "core" ó%6q } +38 "syringe1"C=% +5 "core" ý%6p z +45 "syringe1"C&% +4 "core" æ%6g +46 "syringe1"C2% +3 "core" ò%6i +47 "syringe1"C;% +3 "core" û%6k +47 "syringe1"C7% +4 "core" ÷%6r +47 "syringe1"C#% +3 "core" ã%6o +47 "syringe1"C1% +6 "core" ñ%6r +44 "syringe1"C% +3 "core" Ó%6o +48 "syringe1"C@% +3 "core" %6m +46 "syringe1"CE% +3 "core" %6l +47 "syringe1"C?% +4 "core" ÿ%6 +4 "Can"l_,yu&PVR + #"PROCESSING"Syringe tare is 50% of full scale at 24C (drifted -0%) +7 .PinPort.in :locking +4 "valve1"E? B22 +8 ."PROCESSING"PinPort.seek :locked +3 "valve1"ED( N +82 "core" ( +5 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +9 ."posLyS2"Lysis2.in :fast +6 ."PROCESSING"Lysis1.seek 950uL +6 ."posArchive"Archive.seek :empty +7 "syringe0"B>d P\\k<2 +7 "syringe1"C:( +2 "syringe0"B'( +12 ."posLyS2"Lysis2.seek 950uL +9 "syringe0"BF' +339 "core" ú( +165 ' +310 §( +3 ."PROCESSING"delayUntil "16:02:49.53" +489 "Can"i_,yuBWM +11971 j_P,yuEZo +11969 k_",yuB] +2111 #"bill"-> exit +3 #Session Ended +9853 "Can"j_,yuA`0 +11966 j^,yuCrcN +9882 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +6 BK% +4 "core" %zh +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +9 CH& +5 "core" &z | +48 ."Lysis2mixer"Lysis2.seek 700uL +10 ."PROCESSING"Lysis1.seek 700uL +3 "syringe0"BM'| +5 "syringe1"CO(| +1985 "Can"i^,ys`]h?l +1501 "core" ' +5 ( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +5 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +124 #SPR stabilized +2 "syringe1"CL& +6 "core" &|e~u +3 "syringe0"BA% +6 "core" %|e +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +261 #Waiting up to 11:00 for SPR to enter state 1 +7497 "Can"n^,yu2tl +4269 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BS% +6 "core" %|ep +3 "syringe1"CU& +6 "core" &|eY +48 ."Lysis2mixer"Lysis2.seek 400uL +5 "syringe0"B/' +3 ."PROCESSING"Lysis1.seek 400uL +10 "syringe1"CI( +4174 "core" ¯' +8 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +340 #Waiting up to 1:00:00 for SPR to enter state 7 +1395 "Can"m^,yt.vtW +11963 j^,yu2axi +11964 k^0,yu3H{ +11965 j^@,yu2*J  +11966 i^<,yu1t +11964 l^ ,yu2+ > +11961 m^,yu3 \\ +11960 j^",yu2-5 z +11961 j^3,yu0) +363 #"SPRlogger"SPRsummary:6.05RIU,271RIU,294RIU,385RIU after 1924s +11600 "Can"k^%,yu# +11963 l],yu# +11967 k],yu2 +11966 l],yu#\  +11966 k],yu#\ . +11968 l]\\,yu.\ L +11967 k]`,yu2k\ j +2293 "syringe1"CW& +3 "core" &KcQ 0 +3 "syringe0"B>% +6 "core" ¾%L8 +2 ."PROCESSING"delay 20 seconds +2037 .SPR.command "start 2 0" +274 #Waiting up to 11:00 for SPR to enter state 1 +7349 "Can"j]_,yu1\ +4418 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"BG% +7 "core" %L$D  +4 "syringe1"CN& +4 "core" &KL 1 +48 ."Lysis2mixer"Lysis2.seek 100uL +7 ."PROCESSING"Lysis1.seek 100uL +6 "syringe0"BJ'ނ +4 "syringe1"CC(ނ +4181 "core" ' +4 ( +3 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +291 #Waiting up to 1:00:00 for SPR to enter state 7 +1348 "Can"n]P,yt./\ +11967 k]F,yu1O\ +11966 l]Q,yu3m\ +11967 m]o,yu0G +11968 k],yu1  +11968 j],yu3: < +11969 j],yu6 Z +11966 j],yu1[ x +11968 n^ ,yu/ +11965 j],yu$\  +11967 l^,yu $E> +11967 l^O,yu/;a +11965 o^\\,yu $  +11968 l^X,yu #C , +11967 k^q,yu-H J +11964 j^,yu0 h +2399 #"PROCESSING"SPR analysis completed +60 #"SPRlogger"Ended log spr26aug0115h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +106 "carousel0"FB% +4 "core" %/FPp \ +6 "sampler0"H"% +4 "core" Ȣ%N' +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E]& +5 "core" & N;*OE t +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +3 "valve1"EZ( +81 "core" ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 FT% +3 "core" %/DPQ \ +4 ."detached from bill"Carousel.home.seek 50 +2 "carousel0"F`'/ +17 "core" ' +2 "carousel0"FQ% +4 "core" %/LBQ5  +3 "syringe1"C<& +5 "core" ü&ނ2g8 +3 ."detached from bill"Plunge.seek :locked +3 "valve0"Db'Z +6 "core" ' +3 "syringe0"Bc& +4 "core" &)m; +5 ."Archive"Archive.in :unlocking +3 "syringe1"Ce% +3 "syringe0"B? PZ-2 +8 ."Archive"Archive.seek :clear +4 "syringe0"Bf(j +6 "core" %6u7  +6 ."Air"Air.in :unlocking +3 "syringe1"Ch& +2 C> P]z-2 +8 "core" &ނ2# +2 ."Air"Air.seek :clear +6 "syringe1"Cj' +6 ."Lysis1"Lysis1.in :unlocking +5 "syringe0"BV% +5 "syringe1"C? P[(2 +54 "core" %ނ3o +3 ."Lysis1"Lysis1.seek :clear +9 "syringe1"C*( +5 ."Lysis2"Lysis2.in :unlocking +4 "syringe0"B> P\\k(2 +8 "core" ' +2 ."Lysis2"Lysis2.seek :clear +4 "syringe0"Bm'j +325 "core" ( +99 ê( +19 ' +3 "sampler0"HR% +4 "core" %j +11 ."detached from bill"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +5 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785618693.23EDT @EDT1785618693.87 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +309 .Gate.power :main,:ON,limit to 5000mA +2 .Gate.power :analytic1,:ON,limit to 4000mA +13 #Powering core bus +233 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +36 "valve0"DP +3 Da +3 "core"  +7 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +9 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +10 ."detached from bill"Archive.configure :default + "syringe0"B$d PZ<2 +9 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +8 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +5 #"detached from bill"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"Do% +9 D[& +7 "core" %GkO. 9 +6 "carousel0"F\\% +10 "syringe0"Bl& +4 "syringe1"C^% +5 ."Plunge_home"Plunge.home.jog 0 +9 "syringe1"CX& +7 "syringe0"B_% +13 "valve1"EP& +5 "valve0"Dv) +22 "core" &O-  + %NEQ .R + &%{V  %݈sd + &Wvf ] + %ٝBT  + &O- - + ) +7 ."Carousel_home"Carousel.home.jog 0 +10 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +5 "valve0"Dw% +3 ."PinPort_home"PinPort.home.jog 0 +7 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +7 "carousel0"Fp) +4 "syringe0"B{) +3 "syringe1"C+) +4 "valve1"EY* +2 "syringe1"Ck* +15 "valve0"D* +2 "syringe0"B* +19 "core" %`GuO.  + ) + ) ë) + * + * + * * +3 "syringe0"B% +8 "syringe1"C& +5 "valve1"Eg& +2 "syringe0"Br& +13 "valve0"D}& + ."Plunge_home"Plunge.in :homing +6 "syringe1"Cn% +4 "valve0"D> (2 +21 ."Plunge_home"Plunge.jog 1463 +3 "valve0"Dq) +7 "core" ƒ%r٢~D + Þ&Pe  + &#zO  &u%z5 + &O + %݁yY  +11 ."PinPort_home"PinPort.in :homing +5 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +5 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +4 "valve1"E?2 +3 ."Archive_home"Archive.in :homing +7 "syringe0"B> (2 +4 "syringe1"C> -2 +2 "valve0"D?2 +3 "syringe1"C? (2 +8 "syringe0"B? -2 +2 ."PinPort_home"PinPort.jog 1244 +11 ."Lysis2_home"Lysis2.jog 1619 +6 ."Toroid_home"Toroid.jog 1360 +4 ."Air_home"Air.jog 1433 +6 ."Lysis1_home"Lysis1.jog 1602 +5 ."Archive_home"Archive.jog 1616 +5 "valve1"Es* +5 "syringe0"Bz)S +3 "valve0"Du*P +9 "syringe1"Cx) +2 C*B + "syringe0"B*P +20 "core" ) +3 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +5 "valve0"Di' +24 "core" ' +52 ."Plunge_home"Plunge.in :default +6 "valve0"D> P ޤ-2 +6 "core" ) + ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +6 ."Air_home"Air.setRawPosition! 0 +3 "syringe0"B72 +2 "syringe1"C7 +8 ."Lysis2_home"Lysis2.seek :home +3 ."Air_home"Air.seek :home +6 "syringe0"B|' +4 "syringe1"C' +12 "core" Î* + š* * + Õ' +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +5 ."Air_home"Air.in :default +5 ."PinPort_home"PinPort.setRawPosition! 3150 +4 ."Archive_home"Archive.setRawPosition! 50 +3 "syringe1"C82 +2 C>d P]z<2 +5 "valve1"E8 N +7 "syringe0"B82 +3 ."Lysis1_home"Lysis1.seek :home +9 ."PinPort_home"PinPort.seek :home +3 ."Archive_home"Archive.seek :home +5 "syringe1"C ( +5 "valve1"E ( +4 "syringe0"B( +12 "core" ' +3 ."Lysis2_home"Lysis2.in :default +2 "syringe0"B>d P\\k<2 +12 "core" É( +3 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +6 "core" ™( +3 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +169 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +10 ."Toroid_home"Toroid.seek :home +3 "valve0"D\ ( +7 "core" Ċ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +190 "core" Ō( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +5 "valve1"E& +4 "valve0"D( +5 "core" ş&O& +5 ."detached from bill"PinPort.in :unlocking + "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E( +43 "core" Ň( +290 Ą( +2 ."detached from bill"Air.seek :clear +3 "syringe1"C' +9 "core" Ô' +2 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C( +69 "core" Æ( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B 'j +77 "core" ' +2 ."detached from bill"Archive.seek :clear +3 "syringe0"B(j +76 "core" ’( +3 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F% +3 "core" Ƒ%/QFP  +4 ."detached from bill"Carousel.home.seek 50 +5 "carousel0"F('/ +14 "core" ƨ' +2 "valve0"Dy% +4 "core" %^O; !k +4 #"detached from bill"Selecting Cartridge 48 +2 "valve0"Dd% +6 "core" %^O3 ! +4 "syringe1"C,& +5 "core" ì&XfL +2 "syringe1"C-% +4 "core" í%ݜe[ +3 "valve1"Et& +5 "core" &O5 I +3 ."detached from bill"Carousel.seek 48 +3 "carousel0"F'- +65 "core" Ɩ' +5 "carousel0"F0% +3 "core" ư%-4ʽT 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +6 "syringe1"C & +4 "core" à&ccW +3 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from bill"Lysis1.seek :locked +3 "syringe1"C( +250 "core" È( +3 "valve0"D% +6 "core" Ă%^Oa !| +3 ."Plunge"Plunge.seek :locked +3 "valve0"D'Z +8 "syringe1"C% +7 "core" Ï%ݏb +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C$'6 +79 "core" ą' +25 ä' +3 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F4% +3 "core" ƴ%-[ʼQ  +6 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" 5y +4 "cartridge" yrr +6 "Cartridge" 6y  +6 "cartridge" y . &0J59984 u &0CCu +4 #"detached from bill"Mounted lysis Cartridge board#J59984 +9 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +8 "valve0"D8% +4 "core" ĸ%ZG\ O- R +43 "syringe1"C9& +4 "core" ù&\\> +3 "syringe1"C% +4 "core" Ø%6; +3 "carousel0"F%% +3 "core" ƥ%-ʼPo \ +13 "carousel0"F% +7 "core" Ɲ%-ʼP~ \ +120 ."PROCESSING"SPR.command "read_sequences" +65 .SPR.command "stats_on" +10 "carousel0"F % +4 "core" Ƌ%-ʽPU \ +5 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26aug0117h.spr.bz2 +196 #"PROCESSING"Waiting for baseline SPR stabilization +3 .Handoff.select :waste +6 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C3% +7 "core" ó%6W: +44 "syringe1"C=% +3 "core" ý%6U; +47 "syringe1"C&% +3 "core" æ%6W; +47 "syringe1"C2% +4 "core" ò%6X7 x +46 "syringe1"C;% +4 "core" û%6ER +46 "syringe1"C7% +3 "core" ÷%6WC +47 "syringe1"C#% +3 "core" ã%6W? +47 "syringe1"C1% +3 "core" ñ%6Y2 +48 "syringe1"C% +3 "core" Ó%6T) +47 "syringe1"C@% +3 "core" %6Q$ +47 "syringe1"CE% +3 "core" %6T  +47 "syringe1"C?% +5 "core" ÿ%6N\ | +45 "syringe1"C)% +3 "core" é%6O +48 "syringe1"C.% +4 "core" î%6Y ~ +45 "syringe1"C% +4 "core" Ü%6Q +46 "syringe1"C% +3 "core" Û%6L+ +47 "syringe1"CK% +3 "core" %6L2 +47 "syringe1"CH% +3 "core" %6P2 +47 "syringe1"CF% +3 "core" %6Q2 +47 "syringe1"C:% +3 "core" ú%6Q3 } +47 "syringe1"CL% +3 "core" %6O; +47 "syringe1"CA% +3 "core" %6M5 ~ +47 "syringe1"CS% +3 "core" %6K/ +47 "syringe1"CU% +3 "core" %6N( +47 "syringe1"CM% +4 "core" %6P +46 "syringe1"CO% +3 "core" %6N +4 "Can"j^,yu" ~ +2 #"PROCESSING"Syringe tare is 50% of full scale at 24C (drifted -0%) +3 .PinPort.in :locking +4 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"EW( N +80 "core" ( +4 ."posArchive"Archive.in :fast +7 ."posLyS2"Lysis2.in :fast +10 ."PROCESSING"Lysis1.seek 950uL +4 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +3 "syringe1"C>( +10 ."posLyS2"Lysis2.seek 950uL +7 ."posArchive"Archive.seek :empty +8 "syringe0"BG' +6 BN( +332 "core" þ( +166 ' +334 ( +3 ."PROCESSING"delayUntil "17:22:13.00" +2177 "Can"j^,yu<t +11963 l^,yu?4 +11968 j^,yu;d +11966 n_=,yu; +11968 l_v,yu4 +7603 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +8 B/% +4 "core" ¯%zF: +4 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 CI& +4 "core" &zI x +48 ."PROCESSING"Lysis1.seek 700uL +3 ."Lysis2mixer"Lysis2.seek 700uL +6 "syringe1"CB(| +5 "syringe0"B"'| +3486 "core" ( +2 ¢' +3 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +126 #SPR stabilized + "syringe1"C]& +6 "core" &|e~e' +7 "syringe0"BT% +7 "core" %|eh0 +2 ."PROCESSING"delay 20 seconds +74 "Can"l_,yu0^P  +1928 ."PROCESSING"SPR.command "start 2 0" +258 #Waiting up to 11:00 for SPR to enter state 1 +9781 "Can"l_,yu/ : +1986 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BQ% +6 "core" %|ek4 +6 "syringe1"C<& +4 "core" ü&|e{iO +51 ."Lysis2mixer"Lysis2.seek 400uL +6 ."PROCESSING"Lysis1.seek 400uL +6 "syringe0"Bc' +3 "syringe1"Ce( +4182 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +288 #Waiting up to 1:00:00 for SPR to enter state 7 +3772 "Can"k_,yt. X +11965 k_,yu* v +11966 l_,yu, " +11962 m_,yu/1 +11966 l`,yu1 ! +11967 l_,yu2"# +11966 l_,yu/"'H +11967 l_,yu/** +10027 #"SPRlogger"SPRsummary:4.73RIU,262RIU,252RIU,353RIU after 1924s +1943 "Can"i_,yu$f.cH +11969 k_,yu\ $2f +11967 n_g,yu #6 +11964 l_ ,yu': +11961 l_,yu $Q> +11964 k_,yu\ #B +11968 j^,xyu-+Fy +11953 "syringe1"Ch& +5 "core" &KfI ( +3 "syringe0"BV% +5 "core" %L92  +3 ."PROCESSING"delay 20 seconds +2 "Can"j^,xyu1I +2000 ."PROCESSING"SPR.command "start 2 0" +307 #Waiting up to 11:00 for SPR to enter state 1 +9661 "Can"k],~yu0Ms8 +2107 ."PROCESSING"SPR.command "hold_on" +191 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BD% +7 "core" %L, + +4 "syringe1"C~& +6 "core" &KH - +54 ."Lysis2mixer"Lysis2.seek 100uL +5 ."PROCESSING"Lysis1.seek 100uL +6 "syringe0"B!'ނ +5 "syringe1"C'(ނ +4178 "core" ¡' +6 ç( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +328 #Waiting up to 1:00:00 for SPR to enter state 7 +3562 "Can"l]p,yt.UV +11963 l\\,yu-fYAt +11965 k\\,yu0\\ +11965 n\\B,yu0`< +11964 m[,yu0c +12008 j[,yu-gi +11926 j[,yu.j\ +11965 k[&,yu,nc( +11966 kZ,yu $rF +11965 jZa,yu\ #vBd +11967 jY,yu#z{ +11967 kY,yu+8~| +11966 lY,yu #D +11967 lY4,yu $H{ +11968 mX,yu./ +11967 kX,yu1 +103 #"PROCESSING"SPR analysis completed +66 #"SPRlogger"Ended log spr26aug0117h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +96 "carousel0"F% +5 "core" Ɨ%-ʽPZ \ +8 "sampler0"HC% +4 "core" %_G +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +5 "valve1"EJ& +6 "core" & N;OB  +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +3 "valve1"ER( +80 "core" ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +7 Fa% +3 "core" %-ʽrPO \ +4 ."detached from bill"Carousel.home.seek 48 +7 "carousel0"Fo'- +17 "core" ' +2 "carousel0"FP% +4 "core" %-WʼQB  +3 "syringe1"C_& +4 "core" &ނ2qe +4 ."detached from bill"Plunge.seek :locked +3 "valve0"DX'Z +6 "core" ' +3 "syringe0"B^& +5 "core" &)rj +5 ."Archive"Archive.in :unlocking +3 "syringe1"Cl% +5 "syringe0"B? PZ-2 +5 "core" %6yn +3 ."Archive"Archive.seek :clear +8 ."Air"Air.in :unlocking +4 "syringe0"B\\(j +4 "syringe1"C[& +2 C> P]z-2 +8 ."Air"Air.seek :clear +4 "syringe1"Cw' +7 "core" &ނ2f +5 ."Lysis1"Lysis1.in :unlocking +4 "syringe0"Bg% +4 "syringe1"C? P[(2 +5 "core" %ނ3r: +3 ."Lysis1"Lysis1.seek :clear +6 "syringe1"C( +5 ."Lysis2"Lysis2.in :unlocking +6 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B'j +42 "core" ' +350 ( +35 Ã( +15 ž' +4 "sampler0"Hn% +4 "core" % T +11 ."detached from bill"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785623456.15EDT @EDT1785623456.80 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +308 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +9 #Powering core bus +239 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +25 "valve0"DP +3 Dr +3 "core"  +6 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default + "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +10 ."detached from bill"Carousel.configure :default + "carousel0"F#<` F 22 +10 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +8 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +8 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +13 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"D}% +5 "core" %FO,  +6 "valve0"Db& +4 "carousel0"F`% +5 ."Plunge_home"Plunge.home.jog 0 +13 "syringe0"Bm& +6 "syringe1"Cj% +4 CZ& +5 "syringe0"B*% +3 "valve0"D) + "valve1"Ef& +25 "core" &O'  + %LʹQ' 6 &!~  + %݇~ + &]<~ W + ª%٠9u  + &*'N , Đ) +4 ."Lysis1_home"Lysis1.home.jog 0 +13 "valve0"Dv% +3 ."Archive_home"Archive.home.jog 0 +6 ."Toroid_home"Toroid.home.jog 0 +7 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +4 "syringe1"CY* +4 ."PinPort_home"PinPort.home.jog 0 +9 "syringe0"B{* +3 "valve0"D* +2 "syringe0"B+) +6 "syringe1"Ck) +5 "carousel0"F) +4 "valve1"Eq* +24 "core" %LGO,  + * * + * + «) + ) * + Ɓ) +6 "valve1"Ez& +3 "syringe0"Bx% +4 "syringe1"C& +5 "syringe0"Bs& +6 "valve0"D& +5 "syringe1"C% +3 ."Plunge_home"Plunge.in :homing +2 "valve0"D> (2 +24 ."Plunge_home"Plunge.jog 1487 +9 "valve0"Du) +3 "core" &!O" +2 %s٠yd  Î&Q=F  + &t|o  + Ě&O$ + ß%݇|f +5 ."Air_home"Air.in :homing +11 ."Lysis2_home"Lysis2.in :homing +3 ."Toroid_home"Toroid.in :homing +6 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +5 ."PinPort_home"PinPort.in :homing +3 "syringe1"C> -2 +4 "syringe0"B> (2 +2 "valve0"D?2 +3 "syringe1"C? (2 +7 "syringe0"B? -2 + "valve1"E?2 +9 ."Air_home"Air.jog 1431 +9 ."Lysis2_home"Lysis2.jog 1619 +8 ."Lysis1_home"Lysis1.jog 1602 +6 ."Archive_home"Archive.jog 1619 +4 ."Toroid_home"Toroid.jog 1360 +5 ."PinPort_home"PinPort.jog 1241 +5 "syringe1"C ) +2 "syringe0"B\ )S +5 "syringe1"C*B +12 "valve0"Dd*P +2 "syringe0"By*S + "valve1"E* +25 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"D,' +25 "core" Ĭ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +28 "core" Ì) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +6 ."Air_home"Air.seek :home +3 "syringe1"C-' +7 "core" Š) +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +4 "syringe0"B72 +6 ."Lysis2_home"Lysis2.seek :home +4 "syringe0"B ' +7 "core" í' + Ñ* + * +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Air_home"Air.in :default +4 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe1"C82 +4 C>d P]z<2 +4 "syringe0"B82 +4 ."Lysis1_home"Lysis1.seek :home +9 ."Archive_home"Archive.seek :home +3 "syringe1"Ct( +5 "syringe0"B|( +13 "core" ‰' + ŕ* +2 ."PinPort_home"PinPort.setRawPosition! 3150 +2 ."Lysis2_home"Lysis2.in :default +5 "valve1"E8 N +5 "syringe0"B>d P\\k<2 +47 ."PinPort_home"PinPort.seek :home +14 "valve1"E0( +8 "core" ( + ( +3 ."Archive_home"Archive.in :default +3 ."Lysis1_home"Lysis1.in :default +4 "syringe0"B?d PZ<2 +3 "syringe1"C?d P[<2 +158 "core" * +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +5 "core" Ġ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +231 "core" Ű( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +36 ."clearToroid"Toroid.seek :clear +4 "valve1"E& +5 "valve0"Dp( +4 "core" ř&.O +6 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E(( +42 "core" Ũ( +294 ( +3 ."detached from bill"Air.seek :clear +4 "syringe1"C' +5 "core" Â' +3 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C( +69 "core" Ï( +3 ."detached from bill"Lysis2.seek :clear +5 "syringe0"B'j +75 "core" †' +3 ."detached from bill"Archive.seek :clear +3 "syringe0"B4(j +77 "core" ´( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fi% +3 "core" %-TʺP  +5 ."detached from bill"Carousel.home.seek 48 +7 "carousel0"F'- +15 "core" Ɣ' +2 "valve0"D5% +4 "core" ĵ%^O1 ! +4 #"detached from bill"Selecting Cartridge 46 +2 "valve0"D6% +7 "core" Ķ%^O/ !w +3 "syringe1"C8& +5 "core" ø&[gc +3 "syringe1"C9% +5 "core" ù%ݖbs +2 "valve1"E& +4 "core" Ř&O1 ? +4 ."detached from bill"Carousel.seek 46 +3 "carousel0"F%'+ +64 "core" ƥ' +5 "carousel0"F% +3 "core" Ɲ%+'KS 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C & +4 "core" Ë&ad} +4 ."detached from bill"Lysis1.in :fast + "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +4 "syringe1"C3( +250 "core" ó( +4 "valve0"D=% +7 "core" Ľ%^O[ !i +3 ."Plunge"Plunge.seek :locked +2 "valve0"D&'Z +8 "syringe1"C2% +8 "core" ò%ݖ` +2 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +5 ."Air"Air.seek :locked +3 "syringe1"C;'6 +81 "core" Ħ' +23 û' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F7% +3 "core" Ʒ%+THEP  +6 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +8 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" #y +5 "cartridge" y|(g +4 "Cartridge" 1y ' +5 "cartridge" y /J59919 Ǔ/+i F L/C0CǍ +3 #"detached from bill"Mounted lysis Cartridge board#J59919 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"D% +5 "core" ē%ZGO* W +3 "syringe1"C@& +5 "core" &^m +3 "syringe1"CE% +5 "core" %6` +2 "carousel0"F?% +4 "core" ƿ%+H{PO \  +3 "carousel0"F)% +6 "core" Ʃ%+GzPd \  +118 ."PROCESSING"SPR.command "read_sequences" +7 .SPR.command "stats_on" +8 "carousel0"F.% +4 "core" Ʈ%+GmPe \  +5 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26aug0118h.spr.bz2 +192 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +6 "valve0"D +101 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +8 "syringe1"C% +5 "core" Ü%6[O +45 "syringe1"C% +4 "core" Û%6U_ +46 "syringe1"CK% +4 "core" %6Xg +46 "syringe1"CH% +5 "core" %6Vj { +45 "syringe1"CF% +4 "core" %6Z] { +46 "syringe1"C:% +3 "core" ú%6X^ x +47 "syringe1"CL% +3 "core" %6\\Y } +47 "syringe1"CA% +4 "core" %6X\\  +47 "syringe1"CS% +4 "core" %6Te  +46 "syringe1"CU% +4 "core" %6Tf +47 "syringe1"CM% +3 "core" %6W\\ } +46 "syringe1"CO% +3 "core" %6YQ +47 "syringe1"C% +3 "core" Ç%6US +47 "syringe1"C% +4 "core" È%6RI +4 "Can"kX,yu(}'b +2 #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +3 .PinPort.in :locking +4 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +2 "valve1"E ( N +81 "core" ō( +4 ."posArchive"Archive.in :fast +5 ."posLyS2"Lysis2.in :fast +8 "syringe0"B?d PZ<2 +5 ."PROCESSING"Lysis1.seek 950uL +6 "syringe0"B>d P\\k<2 +7 ."posArchive"Archive.seek :empty +5 "syringe1"C( +5 "syringe0"B/( +5 ."posLyS2"Lysis2.seek 950uL +15 "syringe0"BI' +340 "core" Ò( +160 ' +315 ¯( +2 ."PROCESSING"delayUntil "18:41:34.96" +5180 "Can"nX,yu56 +11971 jX,yu<N:T +11967 iX,yu>nr +11970 jX,yu9X +11972 jX,yu5 +5143 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +6 B>% +3 "core" ¾%zH +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 CG& +4 "core" &zF  +54 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 700uL +6 "syringe0"B]'| +5 "syringe1"CT(| +3484 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +4 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +120 #SPR stabilized +2 "syringe1"CQ& +7 "core" &|ee4 +3 "syringe0"B<% +7 "core" ¼%|ehE +4 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +258 #Waiting up to 11:00 for SPR to enter state 1 +277 "Can"lX,yu-w +11489 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B"% +9 "core" ¢%|egB +4 "syringe1"CB& +5 "core" &|ec\\ +52 ."Lysis2mixer"Lysis2.seek 400uL +6 ."PROCESSING"Lysis1.seek 400uL +6 "syringe0"Bh' +3 "syringe1"CV( +251 "Can"hX,ys%rw> +3929 "core" ' +6 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +287 #Waiting up to 1:00:00 for SPR to enter state 7 +6240 "Can"lX,yu.P +11969 jX,yu/& +11968 jX,yu2LD +11967 kX,yu/b +11962 kX,yu.I +11970 kY,yu/ +11969 kY,yu/t +11983 jY ,yu. +7529 #"SPRlogger"SPRsummary:3.17RIU,263RIU,253RIU,348RIU after 1924s +4430 "Can"mYB,yu$g̽ +11970 jYQ,yu # +11974 kY7,yu\ $f54 +11970 lY^,yu,FR +11971 kYh,yu #p +11972 kYp,yu, +11971 lY,yu) +9432 "syringe1"CD& +5 "core" &KK * +4 "syringe0"B~% +5 "core" %Ly , +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +213 "Can"kY,yu/ +11554 ."PROCESSING"SPR.command "hold_on" +137 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Bc% +6 "core" %L_ * +5 "syringe1"Ce& +5 "core" &K\\ ) +55 ."Lysis2mixer"Lysis2.seek 100uL +4 ."PROCESSING"Lysis1.seek 100uL +8 "syringe0"B'ނ +4 "syringe1"CC(ނ +191 "Can"hY,ys1o +3990 "core" —' +3 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +287 #Waiting up to 1:00:00 for SPR to enter state 7 +6186 "Can"jY,yu.# +11972 lY,yu.h$ +11973 mY,yu1B +11973 mY,yu1ka` +11972 mY,yu-~ +11970 lY,yu. +11970 kY,yu1  +11971 kZ ,yu-  +11970 lZ,yu\ $1O +11971 mZC,yu # +11973 lZ^,yu #2 +11970 jZY,yu-+P +11971 iZq,yu $ n +11970 lZ,yu+7$ +11967 mZ,yu.(4 +9469 #"PROCESSING"SPR analysis completed +68 #"SPRlogger"Ended log spr26aug0118h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +91 "carousel0"FJ% +3 "core" %+FxP= \  +9 "sampler0"Ha% +5 "core" %_W +5 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"EP& +5 "core" & N;O5 +4 ."detached from bill"PinPort.in :unlocking + "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E_( +80 "core" ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F^% +3 "core" %+GPl \  +4 ."detached from bill"Carousel.home.seek 46 +3 "carousel0"Fl'+ +17 "core" ' +2 "carousel0"F[% +4 "core" %+(GS 2 +3 "syringe1"Cg& +5 "core" &ނ2j{ +3 ."detached from bill"Plunge.seek :locked +2 "valve0"DW'Z +8 "core" ' +2 "syringe0"B& +5 "core" –&)e +4 ."Archive"Archive.in :unlocking +4 "syringe1"C% +4 "syringe0"B? PZ-2 +6 ."Archive"Archive.seek :clear +3 "syringe0"BN(j +6 "core" Å%6b } +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +4 C$& +9 ."Air"Air.seek :clear +2 "syringe1"C'' +7 "core" ä&ނ2U +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +4 "syringe0"B!% +9 ."Lysis1"Lysis1.seek :clear +4 "syringe1"Cn( +6 "core" ¡%ނ3mS +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +7 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Br'j +41 "core" ç' +340 ( +40 ( +22 ' +3 "sampler0"H}% +6 "core" %w +13 ."detached from bill"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785628218.08EDT @EDT1785628218.28 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +394 .Gate.power :main,:ON,limit to 5000mA +15 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +32 "valve0"DP +4 D* +3 "core" Ī +8 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default + "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default + "carousel0"F#<` F 22 +9 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +14 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +8 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 ."detached from bill"Air.configure :default +4 "syringe1"C#d P]z<2 +5 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +7 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"DZ% +4 Dj& +3 "core" %FO%  +5 "carousel0"Fm% +5 "syringe0"B`& +4 "syringe1"Cb% +4 Cf& +5 ."Plunge_home"Plunge.home.jog 0 +11 "syringe0"Bv% +3 "valve1"Ex& +5 "valve0"D) +28 "core" &(O" t +2 %NIQ% ?N &m  + %݆- + &Vj ^ + %ٞ'  + &O$ , Ď) +9 "valve0"D% +7 ."PinPort_home"PinPort.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +3 ."Toroid_home"Toroid.home.jog 0 +5 ."Lysis2_home"Lysis2.home.jog 0 +11 ."Air_home"Air.home.jog 0 +3 ."Carousel_home"Carousel.home.jog 0 +4 "valve1"Es* +5 "syringe1"C* +6 "syringe0"Bz* + "valve0"DX* +4 "syringe0"B) + "syringe1"C\\) +6 "carousel0"F) +22 "core" ğ%HFO,  + * + Ú* + * * + ƒ) + ) +2 "syringe0"B% +11 "syringe1"Cw& +6 "valve1"E& +5 "syringe0"Bo& +2 "valve0"DR& +2 "syringe1"Ck% +6 ."Plunge_home"Plunge.in :homing +2 "valve0"D> (2 +20 ."Plunge_home"Plunge.jog 1490 +2 "valve0"D+) +7 "core" Ƅ) + ž%sٟg  + &Xvo  + Ő&O  &r_  + &#O  + %݊dx +12 ."PinPort_home"PinPort.in :homing +8 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +4 ."Archive_home"Archive.in :homing +3 ."Toroid_home"Toroid.in :homing +6 ."Lysis1_home"Lysis1.in :homing +4 "valve1"E?2 +4 "syringe0"B> (2 +5 "syringe1"C> -2 +2 "syringe0"B? -2 +4 "valve0"D?2 + "syringe1"C? (2 +11 ."PinPort_home"PinPort.jog 1239 +13 ."Lysis2_home"Lysis2.jog 1620 +6 ."Lysis1_home"Lysis1.jog 1601 +4 ."Air_home"Air.jog 1431 +4 ."Toroid_home"Toroid.jog 1349 +5 "valve1"EY* +6 ."Archive_home"Archive.jog 1620 +7 "syringe0"B{)T +5 "syringe1"C*A +4 Cq) +3 "valve0"Du*E +8 "syringe0"B *T +9 "core" ī) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +4 "valve0"D\ ' +24 "core" Ċ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +55 "core" ) +3 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +7 ."Air_home"Air.seek :home +3 "syringe1"C' +12 "core" ) + * + Œ* + Ñ' +3 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 ."Archive_home"Archive.setRawPosition! 50 +7 ."Air_home"Air.in :default +4 "syringe0"B72 +4 "syringe1"C82 +7 "syringe0"B82 + "syringe1"C>d P]z<2 +2 ."Lysis2_home"Lysis2.seek :home +3 ."Lysis1_home"Lysis1.seek :home +6 "syringe0"By' +5 "syringe1"C( +9 ."Archive_home"Archive.seek :home +11 "syringe0"B( +50 "core" * + ' + Õ( +5 ."PinPort_home"PinPort.setRawPosition! 3150 +12 ."Lysis2_home"Lysis2.in :default +4 "Can"lZ,yt6=+ +3 ."Lysis1_home"Lysis1.in :default +4 "valve1"E8 N +5 "syringe0"B>d P\\k<2 +2 "syringe1"C?d P[<2 + ."PinPort_home"PinPort.seek :home +10 "valve1"Ed( +12 "core" ™( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +127 "core" * +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +6 "core" Ġ( +3 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +270 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"E0& +5 "valve0"Di( +5 "core" Ű&GO +6 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E5( +42 "core" ŵ( +289 ( +3 ."detached from bill"Air.seek :clear +3 "syringe1"C6' +6 "core" ö' +4 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C-( +69 "core" í( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B8'j +76 "core" ¸' +3 ."detached from bill"Archive.seek :clear +3 "syringe0"Bt(j +77 "core" ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F9% +3 "core" ƹ%+QJP  +4 ."detached from bill"Carousel.home.seek 46 +2 "carousel0"F'+ +18 "core" Ƙ' +3 "valve0"D % +4 "core" ĉ%^O !~ +4 #"detached from bill"Selecting Cartridge 44 +2 "valve0"D% +7 "core" ĝ%^O !| +2 "syringe1"C & +6 "core" Ë&cZ +2 "syringe1"C|% +7 "core" %ݒW +2 "valve1"E& +4 "core" Ł&O"  +3 ."detached from bill"Carousel.seek 44 +3 "carousel0"F'* +66 "core" Ɣ' +4 "carousel0"F=% +5 "core" ƽ%*0T 2 +2 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C2& +3 "core" ò&\\Uw +4 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +3 "syringe1"C( +250 "core" Ï( +3 "valve0"D7% +8 "core" ķ%^O !l +2 ."Plunge"Plunge.seek :locked +2 "valve0"D,'Z +9 "syringe1"C% +7 "core" Â%݊T +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +2 "syringe1"C#'6 +80 "core" Ĭ' +25 ã' +3 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +6 F1% +4 "core" Ʊ%*ZFQ  +7 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" y +6 "cartridge" ym5m5 +3 "Cartridge" @y  +4 "cartridge" y hr/J59915 r/C\\ +3 #"detached from bill"Mounted lysis Cartridge board#J59915 +7 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +11 "valve0"DE% +5 "core" %ZGO! M +3 "syringe1"C?& +5 "core" ÿ&K +2 "syringe1"C)% +5 "core" é%6N +2 "carousel0"F.% +4 "core" Ʈ%*P{ \  +4 "carousel0"F% +7 "core" Ɯ%*PK \  +116 ."PROCESSING"SPR.command "read_sequences" +53 .SPR.command "stats_on" +9 "carousel0"F% +3 "core" ƛ%*yP\\ \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26aug0119h.spr.bz2 +251 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +6 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CK% +6 "core" %6L} +45 "syringe1"CH% +3 "core" %6Mq +47 "syringe1"CF% +3 "core" %6Ms +47 "syringe1"C:% +4 "core" ú%6Gw +46 "syringe1"CL% +3 "core" %6Ls +47 "syringe1"CA% +3 "core" %6J +47 "syringe1"CS% +3 "core" %6Qr +47 "syringe1"CU% +3 "core" %6F +48 "syringe1"CM% +3 "core" %6K{ +47 "syringe1"CO% +3 "core" %6G~ +48 "syringe1"C% +6 "core" Ç%6H +43 "syringe1"C% +4 "core" È%6Kw +46 "syringe1"C(% +4 "core" è%6H| +46 "syringe1"C3% +4 "core" ó%6ځs +4 "Can"lZ,yu-'6 +2 #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +3 .PinPort.in :locking +4 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"E4( N +80 "core" Ŵ( +3 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +9 ."PROCESSING"Lysis1.seek 950uL +8 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +9 "syringe1"C( +5 "syringe0"B>( +4 ."posLyS2"Lysis2.seek 950uL +12 "syringe0"BG' +343 "core" Æ( +158 ' +320 ¾( +3 ."PROCESSING"delayUntil "20:00:59.30" +7641 "Can"jZ,yu:0 +11969 n[,yu=r3D +11969 k[T,yu=I6y" +11971 j[,tyu9g9@ +11972 k[,pyu6<^ +2772 ."PROCESSING"Lysis2.in :DAmix +3 "syringe0"B>d2 P\\k<2 +6 BI% +4 "core" %zM +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 C& +3 "core" Ò&z; +55 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 700uL +8 "syringe0"BQ'| +2 "syringe1"C<(| +3485 "core" ' +4 ü( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +4 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +175 #SPR stabilized + "syringe1"C"& +6 "core" â&|ezL +3 "syringe0"BB% +6 "core" %|eX +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +304 #Waiting up to 11:00 for SPR to enter state 1 +2555 "Can"j[,qyu/B| +9212 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B]% +7 "core" %|eWd +4 "syringe1"CT& +6 "core" &|euXr +51 ."Lysis2mixer"Lysis2.seek 400uL +5 ."PROCESSING"Lysis1.seek 400uL +11 "syringe0"BD' +4 "syringe1"C~( +2530 "Can"h[,pysv;HN +1649 "core" ' +12 ( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +278 #Waiting up to 1:00:00 for SPR to enter state 7 +8529 "Can"k[,tyu/Nq +11973 i[,qyu0Q +11972 k[,qyu. Us +11969 j\\,nyu0X +11968 k\\,jyu/\\t0 +11972 k\\+,cyu-`#N +11970 j\\ ,nyu/cl +11969 k[,tyu/g& +5235 #"SPRlogger"SPRsummary:4.43RIU,268RIU,251RIU,350RIU after 1926s +6733 "Can"k[,~yu$gk +11973 m[d,yu $oU +11972 k[F,yu$s +11969 l[&,yu,pwK +11967 j[ ,yu $.{] +11965 m[,yu([> +11964 l[,yu1\\ +7148 "syringe1"Cc& +3 "core" &KD 8 +3 "syringe0"Be% +6 "core" %L ' +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +307 #Waiting up to 11:00 for SPR to enter state 1 +2539 "Can"kZ,yu*^z +9229 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"Bh% +7 "core" %L!a  +3 "syringe1"CV& +6 "core" &K 0 +53 ."Lysis2mixer"Lysis2.seek 100uL +6 "syringe0"BJ'ނ +4 ."PROCESSING"Lysis1.seek 100uL +9 "syringe1"Ca(ނ +2460 "Can"jZ,ysu +1710 "core" ' +10 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +290 #Waiting up to 1:00:00 for SPR to enter state 7 +8450 "Can"kZ,yu(. +11966 lZ,yu- +11968 lZ,yu-, +11963 jZ,yu/ +11968 mZ,yu-+. +11967 kZ,yu.L +11969 lZ,yu'Zj +11971 jZ,yu/ +11973 kZ,yu $[ +11972 mZm,yu $\  +11968 nZN,yu\ #? +11967 lZD,yu' +11966 jZ?,yu #\  +11966 kZ\\,}yu+ < +11965 jZI,yu/ƓZ +7251 #"PROCESSING"SPR analysis completed +72 #"SPRlogger"Ended log spr26aug0119h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +80 "carousel0"FP% +3 "core" %*P$ \  +7 "sampler0"H^% +5 "core" %N} +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +3 "valve1"E[& +10 "core" & N;O* +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +2 "valve1"Eg( +81 "core" ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" Ɩ%*"PN \  +4 ."detached from bill"Carousel.home.seek 44 +3 "carousel0"F'* +16 "core" ƅ' +2 "carousel0"F$% +4 "core" Ƥ%*#pS 2 +3 "syringe1"C!& +4 "core" á&ނ2i +4 ."detached from bill"Plunge.seek :locked +2 "valve0"D 'Z +6 "core" č' +4 "syringe0"B%& +3 "core" ¥&)a +5 ."Archive"Archive.in :unlocking +3 "syringe1"C&% +4 "syringe0"B? PZ-2 +8 "core" æ%6d +2 ."Archive"Archive.seek :clear +7 "syringe0"B/(j +3 "syringe1"C;& +9 ."Air"Air.in :unlocking +4 "syringe1"C> P]z-2 +9 "core" û&ނ2 +3 ."Air"Air.seek :clear +7 ."Lysis1"Lysis1.in :unlocking +7 "syringe1"CC' +4 "syringe0"B% +5 "syringe1"C? P[(2 +8 ."Lysis1"Lysis1.seek :clear +2 "syringe1"C}( +7 "core" —%ނ3t +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B*'j +54 "core" ' +326 ¯( +40 ( +22 ª' +4 "sampler0"HZ% +3 "core" %I +11 ."detached from bill"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785632983.34EDT @EDT1785632983.54 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +352 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +237 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +32 "valve0"DP +3 Dx +3 "core"  +7 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +8 ."detached from bill"PhaseSeparator.configure :default +3 "carousel1"G$ +9 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +8 "valve0"Dv% +4 Df& +3 "core" %G 7O+  +4 "carousel0"Fb% +5 "syringe0"B`& +5 "syringe1"Cm% +6 ."Plunge_home"Plunge.home.jog 0 +9 "syringe1"Cj& +6 "syringe0"B% +5 "valve1"E& +3 "valve0"D) +31 "core" &PO L + %NQ G + &   + %ݍv + &Rx b Ÿ%٧x  + Ő&O" , + Ğ) +8 "valve0"Dw% +8 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +6 ."PinPort_home"PinPort.home.jog 0 +5 "syringe0"Bk) +3 "syringe1"CR) +4 "carousel0"Fo) +9 "valve0"DW* +6 "syringe1"C_* +3 "syringe0"Bn* +4 "valve1"EN* +19 "core" %KGO$  + ) + ) + * ) + * + * * +3 "syringe0"Br% +10 B& +5 "valve1"Ep& +3 "syringe1"Cl% +3 "valve0"Ds& +2 ."Plunge_home"Plunge.in :homing +13 "syringe1"C'& +2 "valve0"D> (2 +14 ."Plunge_home"Plunge.jog 1487 +3 "valve0"D) +10 "core" %t٤  + Ž&t>  + &O  %݆z + &RO + ç&O{  +5 ."Lysis2_home"Lysis2.in :homing +12 ."PinPort_home"PinPort.in :homing +5 ."Lysis1_home"Lysis1.in :homing +4 ."Toroid_home"Toroid.in :homing +5 ."Archive_home"Archive.in :homing +5 ."Air_home"Air.in :homing +5 "syringe0"B> (2 +3 "valve1"E?2 +3 "syringe1"C? (2 +5 "valve0"D?2 +4 "syringe0"B? -2 +2 "syringe1"C> -2 +2 ."PinPort_home"PinPort.jog 1238 +6 ."Lysis2_home"Lysis2.jog 1619 +5 "valve1"E* +10 "syringe0"B\\)S +7 ."Lysis1_home"Lysis1.jog 1603 +8 ."Air_home"Air.jog 1432 +8 ."Toroid_home"Toroid.jog 1355 +7 ."Archive_home"Archive.jog 1619 +6 "syringe1"CX*C +5 Cz) + "valve0"D+*K +8 "syringe0"Bq*S +15 "core" ă) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +5 "valve0"D{' +24 "core" ' +4 ."Plunge_home"Plunge.in :default +3 "valve0"D> P ޤ-2 +22 "core" ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +2 "syringe0"B72 +7 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"B' +14 "core" ) +2 ."Air_home"Air.setRawPosition! 0 +3 "syringe1"C7 +6 ."Air_home"Air.seek :home +6 "syringe1"C ' +7 "core" * ' + Ś* + * + Ì' +3 ."PinPort_home"PinPort.setRawPosition! 3150 +5 ."Lysis2_home"Lysis2.in :default +3 ."Air_home"Air.in :default +4 "valve1"E8 N +4 ."Lysis1_home"Lysis1.setRawPosition! 50 +6 ."Archive_home"Archive.setRawPosition! 50 +4 "syringe0"B>d P\\k<2 +4 "syringe1"C>d P]z<2 +4 C82 +2 ."PinPort_home"PinPort.seek :home +3 "syringe0"B82 +5 "valve1"EY( +11 ."Archive_home"Archive.seek :home +6 ."Lysis1_home"Lysis1.seek :home +5 "syringe0"Bu( +3 "syringe1"C0( +59 "core" ( +2 ."Archive_home"Archive.in :default +12 "syringe0"B?d PZ<2 +11 "core" ð( +2 ."Lysis1_home"Lysis1.in :default +2 "syringe1"C?d P[<2 +149 "core" ī* +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +10 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +5 "core" Ġ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +153 "core" ( +3 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"Ed& +5 "valve0"D9( +5 "core" &MO +5 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E ( +42 "core" ʼn( +294 Ĺ( +3 ."detached from bill"Air.seek :clear +3 "syringe1"C' +6 "core" Ý' +4 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C( +70 "core" Ñ( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B 'j +77 "core" ‹' +2 ."detached from bill"Archive.seek :clear +3 "syringe0"B(j +76 "core" •( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F|% +3 "core" %*UQ  +4 ."detached from bill"Carousel.home.seek 44 +3 "carousel0"F'* +16 "core" Ɓ' +2 "valve0"Dy% +5 "core" %^O2 !c +3 #"detached from bill"Selecting Cartridge 26 +2 "valve0"D=% +7 "core" Ľ%^O7 !m +3 "syringe1"C2& +4 "core" ò&Fg +3 "syringe1"C% +4 "core" Ù%݋\\ +3 "valve1"E& +5 "core" ń&O.  +3 ."detached from bill"Carousel.seek 26 +3 "carousel0"F'u +391 "core" Ƙ' +4 "carousel0"F7% +3 "core" Ʒ%u3lT 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C& +3 "core" Â&La +4 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +3 "syringe1"C-( +250 "core" í( +3 "valve0"D1% +11 "core" ı%^O !c +3 ."Plunge"Plunge.seek :locked +2 "valve0"D\ 'Z +8 "syringe1"C6% +6 "core" ö%ݍ` +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C'6 +80 "core" Ċ' +24 Ó' +3 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F@% +3 "core" %u\\ldQ  +6 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +6 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +9 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" Ey +6 "cartridge" yִjִj +4 "Cartridge" ?y  +3 "Can"kZ@,yuNʦx +6 "cartridge" y r/J59943 r/6Cr +3 #"detached from bill"Mounted lysis Cartridge board#J59943 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"D)% +6 "core" ĩ%ZGO$ E +2 "syringe1"C.& +6 "core" î&Y +2 "syringe1"C% +4 "core" Ü%6 ; +3 "carousel0"F% +5 "core" ƛ%ulPc \  +4 "carousel0"FK% +5 "core" %ulPo \ +117 ."PROCESSING"SPR.command "read_sequences" +25 .SPR.command "stats_on" +9 "carousel0"FH% +3 "core" %ulPh \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26aug0121h.spr.bz2 +197 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +3 "valve0"D +53 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +51 "syringe1"CF% +6 "core" %6X > +45 "syringe1"C:% +4 "core" ú%6 Z G +46 "syringe1"CL% +3 "core" %6y = +47 "syringe1"CA% +4 "core" %6 V F +46 "syringe1"CS% +3 "core" %6T A +47 "syringe1"CU% +3 "core" %6\ R H +47 "syringe1"CM% +3 "core" %6T D +47 "syringe1"CO% +3 "core" %6V D +48 "syringe1"C% +3 "core" Ç%6X > +47 "syringe1"C% +3 "core" È%6 T G +47 "syringe1"C(% +3 "core" è%6ȁ > +47 "syringe1"C3% +3 "core" ó%6P~ K +48 "syringe1"C5% +6 "core" õ%6S ; +43 "syringe1"C% +4 "core" Ï%6O B +4 "Can"kZ@,yu { + #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +4 .PinPort.in :locking +4 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +3 "valve1"Et( N +80 "core" ( +4 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +5 ."posLyS2"Lysis2.in :fast +9 ."PROCESSING"Lysis1.seek 950uL +5 "syringe0"B>d P\\k<2 +2 ."posArchive"Archive.seek :empty +11 "syringe1"C8( +7 ."posLyS2"Lysis2.seek 950uL +5 "syringe0"BI( +7 B' +351 "core" ø( +149 ’' +330 ( +2 ."PROCESSING"delayUntil "21:20:24.99" +9723 "Can"jZ,yu>p +11966 kZ,yu7Ѧ +11965 lY,yu= +11965 lY,yu7r +11968 mY,yu; @ +614 ."PROCESSING"Lysis2.in :DAmix + "syringe0"B>d2 P\\k<2 +7 BG% +4 "core" %zJ +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 C& +3 "core" Æ&zF +58 ."Lysis2mixer"Lysis2.seek 700uL +3 "syringe0"B"'| +3 ."PROCESSING"Lysis1.seek 700uL +9 "syringe1"CB(| +3474 "core" ¢' +11 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +122 #SPR stabilized +2 "syringe1"C]& +6 "core" &|exwb +3 "syringe0"BT% +10 "core" %|erd +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +311 #Waiting up to 11:00 for SPR to enter state 1 +4753 "Can"lY,}yu0, +7055 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BQ% +7 "core" %|e_ +3 "syringe1"C<& +6 "core" ü&|e +49 ."Lysis2mixer"Lysis2.seek 400uL +6 ."PROCESSING"Lysis1.seek 400uL +6 "syringe0"Bc' +3 "syringe1"Ce( +4181 "core" ' +6 ( +2 ."PROCESSING"delay 15 seconds +507 "Can"jY,yt/S<J +995 ."PROCESSING"SPR.command "hold_off" +291 #Waiting up to 1:00:00 for SPR to enter state 7 +10687 "Can"jY,yu/h +11968 mZ,yu0X +11964 iZE,{yu2 +11967 mZM,|yu)'[ +11966 lZJ,yyu- +11967 lZ,wyu0 +11968 kY,syu0 +11966 kY,nyu/: +3105 #"SPRlogger"SPRsummary:3.02RIU,240RIU,224RIU,306RIU after 1925s +8859 "Can"kYz,hyu\ $b X +11967 kY_,lyu# v +11972 lYp,dyu\ $0 +11967 iYi,ayu., +11964 iYr,byu # +11963 kY},ayu*b +11967 lY,ayu(!@ +5031 "syringe1"Ch& +3 "core" &KI . +4 "syringe0"BV% +4 "core" %L%  +3 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +4612 "Can"lY,^yu-$* +7155 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BD% +6 "core" %L+  +5 "syringe1"C~& +4 "core" &K 7 +56 ."Lysis2mixer"Lysis2.seek 100uL +7 ."PROCESSING"Lysis1.seek 100uL +3 "syringe0"BP'ނ +3 "syringe1"C^(ނ +4181 "core" ' +5 ( +2 ."PROCESSING"delay 15 seconds +398 "Can"mY,`yt.+H +1105 ."PROCESSING"SPR.command "hold_off" +341 #Waiting up to 1:00:00 for SPR to enter state 7 +10519 "Can"iYq,byu-"0f +11966 mY,_yu,4  +11967 lY,_yu07 +11969 kYo,_yu/;\  +11967 kYb,Zyu0> +11964 kY@,Zyu.B: +11964 jY0,Yyu.E +11965 mY,Xyu-I:8 +11965 iX,[yu\ $MIV +11966 lX,^yu$bQt +11965 iX,^yu #U +11965 kX,^yu Y] +11961 mX,Uyu #] +11966 kX,Vyu-fal +11966 lYb,Tyu.d\ +5254 #"PROCESSING"SPR analysis completed +66 #"SPRlogger"Ended log spr26aug0121h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +95 "carousel0"F[% +4 "core" %ulPR \  +6 "sampler0"H% +4 "core" Ȗ%: +9 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E$& +5 "core" Ť& N:1O m +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +2 "valve1"E!( +81 "core" š( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +7 F%% +4 "core" ƥ%ulPY \ +4 ."detached from bill"Carousel.home.seek 26 +3 "carousel0"F&'u +17 "core" Ʀ' +2 "carousel0"F;% +5 "core" ƻ%u$lS 2 +2 "syringe1"C& +5 "core" ×&ނ2 +3 ."detached from bill"Plunge.seek :locked +5 "valve0"D4'Z +5 "core" Ĵ' +3 "syringe0"B& +4 "core" ”&) +5 ."Archive"Archive.in :unlocking +3 "syringe1"C,% +4 "syringe0"B? PZ-2 +8 ."Archive"Archive.seek :clear +2 "syringe0"B>(j +7 "core" ì%6 h +4 ."Air"Air.in :unlocking +4 "syringe1"C#& +4 C> P]z-2 +7 "core" ã&ނ2ˁ +3 ."Air"Air.seek :clear +8 ."Lysis1"Lysis1.in :unlocking +5 "syringe1"Ca' +2 C? P[(2 +4 "syringe0"BJ% +10 ."Lysis1"Lysis1.seek :clear +2 "syringe1"CZ( +7 "core" %ނ3q +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bx'j +54 "core" ' +326 ¾( +42 ( +22 ' +3 "sampler0"Hv% +4 "core" %C +12 ."detached from bill"Gate.power :motor,:OFF +5 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +3 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785637750.03EDT @EDT1785637750.34 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +344 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +12 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +34 "valve0"DP +3 D +3 "core" ğ +7 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +10 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +8 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +8 ."detached from bill"Abs.configure :default +3 "ambient0"J#  +9 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +10 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +12 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"Dj% +6 "core" %GO+  +2 "valve0"Dm& +10 ."Plunge_home"Plunge.home.jog 0 +6 "carousel0"F`% +6 "syringe0"Bb& +3 "syringe1"Cf% +4 C& +4 "valve0"Dw) +5 "syringe0"Bp% +7 "valve1"Er& +26 "core" &oO* - + %NlQ  + &s  %݆ + Ð&J2 j + %ٖ+  + &6*N -Q ) +9 "valve0"D'% +7 ."PinPort_home"PinPort.home.jog 0 +6 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +6 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +6 "valve1"El* +6 "syringe1"Cs* +3 "syringe0"B* + "valve0"D * +8 "syringe0"B*) +2 "syringe1"CC) +14 "carousel0"F) +13 "core" ħ%OGO- + * + * + Ž* č* + ª) + ) +2 "syringe0"Bi% +7 "syringe1"C}& +4 "valve0"Dg& +3 "valve1"E/& +6 "syringe0"B& +2 "syringe1"CN% +3 ."Plunge_home"Plunge.in :homing +3 "valve0"D> (2 +27 ."Plunge_home"Plunge.jog 1483 +5 "valve0"Dk) +4 "core" ƅ) +2 %sٛj  &F,  + &tO + ů&*(O\ + ž&r h  + %݈) +6 ."Archive_home"Archive.in :homing +11 ."Lysis1_home"Lysis1.in :homing +7 ."Lysis2_home"Lysis2.in :homing +6 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +4 "syringe1"C? (2 +2 ."PinPort_home"PinPort.in :homing +6 "syringe0"B? -2 +4 B> (2 +2 "syringe1"C> -2 +4 "valve0"D?2 +4 ."Lysis1_home"Lysis1.jog 1604 +13 "valve1"E?2 +5 ."Archive_home"Archive.jog 1621 +5 ."Air_home"Air.jog 1431 +5 ."Lysis2_home"Lysis2.jog 1620 +6 ."Toroid_home"Toroid.jog 1358 +5 "syringe1"C_*D +4 ."PinPort_home"PinPort.jog 1251 +3 "syringe0"BR*U +5 "syringe1"CW) +4 "syringe0"Bn)T +11 "valve0"D*N + "valve1"E\\* +23 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +4 "valve0"Dz' +25 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +37 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +2 "syringe1"C82 +6 "core" ) +5 ."Air_home"Air.setRawPosition! 0 +3 ."Lysis1_home"Lysis1.seek :home +6 "syringe1"C7 +3 C( +7 ."Air_home"Air.seek :home +56 "syringe1"Cq' +6 "core" * + ) + Ú( * + ' +3 ."PinPort_home"PinPort.setRawPosition! 3150 +15 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 ."Lysis1_home"Lysis1.in :default +4 ."Archive_home"Archive.setRawPosition! 50 +5 ."Air_home"Air.in :default +5 "valve1"E8 N +3 "syringe1"C?d P[<2 +3 "syringe0"B72 +10 B82 +2 "syringe1"C>d P]z<2 +4 ."PinPort_home"PinPort.seek :home +4 ."Lysis2_home"Lysis2.seek :home +7 ."Archive_home"Archive.seek :home +4 "valve1"EX( +4 "syringe0"Bd' +2 B+( +27 "core" ' +2 ."Lysis2_home"Lysis2.in :default +3 "syringe0"B>d P\\k<2 +5 "core" «( +4 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +128 "core" ă* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +7 "core" Ġ( +2 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +237 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"EY& +4 "valve0"D|( +4 "core" &O  +8 ."detached from bill"PinPort.in :unlocking +3 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"Ey( +43 "core" ( +291 ( +3 ."detached from bill"Air.seek :clear +3 "syringe1"C=' +8 "core" ý' +3 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C ( +69 "core" Ì( +2 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B2'j +78 "core" ²' +2 ."detached from bill"Archive.seek :clear +3 "syringe0"B0(j +76 "core" °( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" ƙ%sTl)P  +7 ."detached from bill"Carousel.home.seek 26 +3 "carousel0"F'u +17 "core" Ƅ' +2 "valve0"D% +6 "core" %^O( !y +3 #"detached from bill"Selecting Cartridge 24 +2 "valve0"Du% +7 "core" %^xO, ! +3 "syringe1"Co& +5 "core" &DY +2 "syringe1"C% +5 "core" Á%ݑ +3 "valve1"E7& +4 "core" ŷ&]O-  +3 ."detached from bill"Carousel.seek 24 +3 "carousel0"F' +65 "core" Ƃ' +5 "carousel0"F1% +3 "core" Ʊ%0deT 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C6& +3 "core" ö&PX +4 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +3 "syringe1"C( +251 "core" Ñ( +3 "valve0"D@% +7 "core" %^}OO ! +3 ."Plunge"Plunge.seek :locked +3 "valve0"D{'Z +7 "syringe1"C% +6 "core" Ý%ݖz +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"CE'6 +80 "core" ' +24 ' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F?% +3 "core" ƿ%ZdOBQ  +6 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +3 "Cartridge" )y +5 "cartridge" y RI +5 "Cartridge" .y  +5 "cartridge" y k`/ C;R c/J59986 +3 #"detached from bill"Mounted lysis Cartridge board#J59986 +5 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +7 "valve0"D% +5 "core" Ĝ%ZGO$ [ +3 "syringe1"C& +6 "core" Û&U +2 "syringe1"CK% +4 "core" %6W | +3 "carousel0"FH% +5 "core" %dNP\\ \ +3 "carousel0"FF% +7 "core" %dOPQ \ +127 ."PROCESSING"SPR.command "read_sequences" +71 .SPR.command "stats_on" +9 "carousel0"F:% +3 "core" ƺ%dP?P@ \ +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26aug0122h.spr.bz2 +250 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CL% +7 "core" %6M +44 "syringe1"CA% +3 "core" %6L { +47 "syringe1"CS% +3 "core" %6N x +47 "syringe1"CU% +4 "core" %6L +46 "syringe1"CM% +3 "core" %6K y +47 "syringe1"CO% +3 "core" %6K{ | +47 "syringe1"C% +3 "core" Ç%6D ~ +47 "syringe1"C% +3 "core" È%6E{ w +47 "syringe1"C(% +4 "core" è%6E u +46 "syringe1"C3% +4 "core" ó%6G +47 "syringe1"C5% +6 "core" õ%6I x +43 "syringe1"C% +4 "core" Ï%6G { +48 "syringe1"C % +3 "core" É%6H y +45 "syringe1"C-% +5 "core" í%6G  +5 "Can"lYb,Tyu!h& + #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +5 .PinPort.in :locking +2 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"E( N +81 "core" ŕ( +3 ."posArchive"Archive.in :fast +3 "syringe0"B?d PZ<2 +6 ."posLyS2"Lysis2.in :fast +9 ."PROCESSING"Lysis1.seek 950uL +5 "syringe0"B>d P\\k<2 +5 ."posArchive"Archive.seek :empty +8 "syringe1"C ( +9 ."posLyS2"Lysis2.seek 950uL +4 "syringe0"BG( +6 B' +345 "core" Ë( +154 †' +258 "Can"lY,Qytq?i}( +74 "core" ( +3 ."PROCESSING"delayUntil "22:39:50.75" +11890 "Can"kY,Ryu5flF +11967 kY,Pyu;Bod +11964 kY,Oyu8Ds% +11966 kY,Pyu<vY +10502 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +6 B% +4 "core" ’%{@n ~ +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +7 C8& +3 "core" ø&z=^ { +55 ."Lysis2mixer"Lysis2.seek 700uL +6 ."PROCESSING"Lysis1.seek 700uL +5 "syringe0"B]'| +7 "syringe1"CT(| +1366 "Can"iY,Mys`zp +2122 "core" ' +6 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +5 "valve0"D@ +52 ."PROCESSING"SPR.command "start 2 14" +111 #SPR stabilized + "syringe1"CQ& +6 "core" &|ena` +3 "syringe0"B<% +6 "core" ¼%|ee_ +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +262 #Waiting up to 11:00 for SPR to enter state 1 +6882 "Can"mYp,Syu0W +4885 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B"% +7 "core" ¢%|en +3 "syringe1"CB& +7 "core" &|e~p +55 ."Lysis2mixer"Lysis2.seek 400uL +4 ."PROCESSING"Lysis1.seek 400uL +6 "syringe0"Bh' +3 "syringe1"CV( +4184 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +285 #Waiting up to 1:00:00 for SPR to enter state 7 +880 "Can"kY2,]yt/$ +11970 kY,byu/4  +11967 jX,eyu-) 6 +11970 mX,gyu07 T +11971 mX,oyu. r +11969 jX,nyu/; +11970 jX,myu( +11967 lX,jyu/i +11968 kX,myu* +926 #"SPRlogger"SPRsummary:3.63RIU,186RIU,167RIU,239RIU after 1925s +11043 "Can"mXp,oyu $&! +11969 lXS,qyu#X!& +11970 jX=,qyu.>!D +11972 kXP,myu $l%!b +11968 jXC,oyu\ $+c! +11971 kX2,oyu.2%! +11970 lX@,oyu.! +2826 "syringe1"CD& +5 "core" &KO - +2 "syringe0"B~% +6 "core" %L6ǁ +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +365 #Waiting up to 11:00 for SPR to enter state 1 +6757 "Can"jXF,oyu,&! +5048 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Bc% +9 "core" %LCU +3 "syringe1"Ce& +58 "core" &KQ 0 +41 ."Lysis2mixer"Lysis2.seek 100uL +5 "syringe0"B['ނ +4 ."PROCESSING"Lysis1.seek 100uL +9 "syringe1"C(ނ +4173 "core" ' +10 Ö( +2 ."PROCESSING"delay 15 seconds +1504 .SPR.command "hold_off" +338 #Waiting up to 1:00:00 for SPR to enter state 7 +621 "Can"jX ,wyt.ʵ! +11970 lW,yu0" +11968 jW,yu-/x"4 +11968 jX,}yu-"R +11969 kX,~yu.w"p +11966 lX,yu1" +11969 kX!,yu/" +11966 mX%,yu) )" +11967 jX<,yyu+" +11964 kXI,zyu$J# +11965 mXP,uyu $#$ +11967 nXe,uyu/ D#B +11966 jXr,qyu $#` +11968 lXz,myt%F#~ +11965 lX,kyu+# +11967 mX,jyu/g# +3123 #"PROCESSING"SPR analysis completed +76 #"SPRlogger"Ended log spr26aug0122h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +68 "carousel0"F$% +4 "core" Ƥ%dN~Pn \ +6 "sampler0"H%% +6 "core" ȥ%? +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +6 "valve1"E;& +4 "core" Ż& N:O2  +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +3 "valve1"E( +80 "core" ŗ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" Ɣ%dNTPG \ +4 ."detached from bill"Carousel.home.seek 24 +3 "carousel0"F,' +19 "core" Ƭ' +2 "carousel0"F#% +4 "core" ƣ%1dYT 2 +3 "syringe1"CJ& +4 "core" &ނ2 +3 ."detached from bill"Plunge.seek :locked +4 "valve0"Dt'Z +6 "core" ' +2 "syringe0"B& +4 "core" ˜&) +10 ."Archive"Archive.in :unlocking +3 "syringe1"C\ % +5 "syringe0"B? PZ-2 +5 "core" Ê%6 +3 ."Archive"Archive.seek :clear +7 "syringe1"CI& +7 ."Air"Air.in :unlocking +3 "syringe0"B(j +2 "syringe1"C> P]z-2 +8 "core" &ނ2 +2 ."Air"Air.seek :clear +9 ."Lysis1"Lysis1.in :unlocking +4 "syringe1"C^' +56 "syringe0"BP% +3 "syringe1"C? P[(2 +10 ."Lysis1"Lysis1.seek :clear +3 "syringe1"Cv( +7 "core" %ނ3j +5 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +5 "syringe0"B'j +3 "core" ' +337 “( +79 ( +22 Ÿ' +4 "sampler0"Hj% +5 "core" %@ +10 ."detached from bill"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +5 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785642516.17EDT @EDT1785642516.82 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +308 .Gate.power :main,:ON,limit to 5000mA +4 .Gate.power :analytic1,:ON,limit to 4000mA +11 #Powering core bus +235 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +4 Dr +3 "core"  +6 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +8 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +10 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +10 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +9 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."detached from bill"Archive.configure :default +3 "syringe0"B$d PZ<2 +8 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +5 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +11 ."detached from bill"Lysis2.configure :default +3 "syringe0"B#d P\\k<2 +9 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +7 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"Dp% +4 D& +6 "carousel0"Ff% +2 "syringe0"Bb& +4 "syringe1"C`% +6 Cm& +8 "syringe0"B'% +16 "valve1"Ei& +5 "core" %GO(  + Đ& \\O% ԏ + %LdVQ! A + &&f  + &L* h %ݍ1 + §%ٶj  +14 ."Lysis1_home"Lysis1.home.jog 0 +12 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +6 ."Plunge_home"Plunge.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +5 "syringe1"C}* +3 CN) +3 "carousel0"F) +2 "syringe0"Bg) +5 B/* +3 "valve0"D4) +4 D!* +32 "core" &WO, -0 +2 * + ) + ƞ) + ¯* ) + Ĵ) + ġ* +3 "syringe0"BZ% +7 "syringe1"C>& +7 ."PinPort_home"PinPort.home.jog 0 +8 "syringe0"B9& +3 "valve0"Da% +4 Dx& +2 "syringe1"C&% +6 "valve1"Ew* +19 "core" %u٢g + þ&JG  + ¹&tj %KGO + &O + æ%݉i + * +2 "valve1"Es& +8 ."Lysis1_home"Lysis1.in :homing +10 ."Air_home"Air.in :homing +6 ."Archive_home"Archive.in :homing +5 ."Plunge_home"Plunge.in :homing +4 ."Lysis2_home"Lysis2.in :homing +5 ."Toroid_home"Toroid.in :homing +4 "syringe1"C? (2 +3 C> -2 +4 "syringe0"B? -2 + "valve0"D> (2 +9 "syringe0"B> (2 +4 "valve0"D?2 +3 ."Lysis1_home"Lysis1.jog 1604 +7 ."Air_home"Air.jog 1431 +4 ."Archive_home"Archive.jog 1618 +5 ."Lysis2_home"Lysis2.jog 1619 +4 ."Toroid_home"Toroid.jog 1348 +5 ."Plunge_home"Plunge.jog 1483 +4 "syringe1"C*D +4 CC) + "syringe0"B**R +12 B )S +8 "valve0"Dl*D +3 Dk) +15 "core" &'PO  +4 ."PinPort_home"PinPort.in :homing + "valve1"E?2 +7 ."PinPort_home"PinPort.jog 1248 +3 "valve1"E_* +66 "core" ) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +6 ."Air_home"Air.seek :home +5 "syringe1"CW' +55 "core" Î* + ª* + ' ) + ) +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +14 ."Air_home"Air.in :default +5 ."Plunge_home"Plunge.setRawPosition! 50 +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +4 ."Archive_home"Archive.setRawPosition! 50 +6 "syringe1"C82 +5 C>d P]z<2 +2 "valve0"D72 +4 "syringe0"B72 +7 B82 +4 ."Lysis1_home"Lysis1.seek :home +10 ."Plunge_home"Plunge.seek :home +6 ."Lysis2_home"Lysis2.seek :home +5 ."Archive_home"Archive.seek :home +4 "syringe1"C\\( +4 "valve0"Dn' +7 "syringe0"BR' +6 BY( +10 "core" * +3 ."PinPort_home"PinPort.setRawPosition! 3150 + "valve1"E8 N +8 ."PinPort_home"PinPort.seek :home +5 "valve1"E( +8 "core" ( + ' + ' ( +5 ."Lysis2_home"Lysis2.in :default +2 ."Lysis1_home"Lysis1.in :default +5 ."Plunge_home"Plunge.in :default +4 ."Archive_home"Archive.in :default +5 "syringe0"B>d P\\k<2 +3 "syringe1"C?d P[<2 +2 "valve0"D> P ޤ-2 +7 "syringe0"B?d PZ<2 +111 "core" * +2 ."Toroid_home"Toroid.setRawPosition! 0 + "valve0"D8 +8 ."Toroid_home"Toroid.seek :home +2 "valve0"D ( +5 "core" Ġ( +5 ."Toroid_home"Toroid.in :default + "valve0"D? ֜2 +256 "core" Ń( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"EX& +3 "valve0"D( +7 "core" &O  +5 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E( +44 "core" ( +292 ę( +3 ."detached from bill"Air.seek :clear +2 "syringe1"Cu' +9 "core" ' +3 ."detached from bill"Lysis1.seek :clear +3 "syringe1"Cq( +69 "core" ( +3 ."detached from bill"Lysis2.seek :clear +4 "syringe0"Bo'j +75 "core" ' +3 ."detached from bill"Archive.seek :clear +3 "syringe0"B(j +77 "core" š( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +6 F% +9 "core" Ɓ%XdWQ  +3 ."detached from bill"Carousel.home.seek 24 +3 "carousel0"F7' +18 "core" Ʒ' +2 "valve0"Dd% +4 "core" %^p O. ! +4 #"detached from bill"Selecting Cartridge 22 +2 "valve0"D1% +7 "core" ı%^{O/ ! +3 "syringe1"C6& +5 "core" ö&UX +3 "syringe1"C+% +4 "core" ë%ݏi +3 "valve1"E& +4 "core" Ņ&O0 x +3 ."detached from bill"Carousel.seek 22 +2 "carousel0"F' +65 "core" Ƅ' +5 "carousel0"F@% +3 "core" %)[T 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C& +3 "core" Ý&O[ +4 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 "core" Ƅ' N[!Q  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 22 + 5 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.019179185076093T: @currentiS: @type2F:@outPressi!:\ @lostF: @inPressi[:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni: @pastRLSF: @tagi : @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +5 ."detached from bill"Lysis1.seek :locked +4 "syringe1"C ( +6 "core" Ƅ' +243 Ì( +3 "valve0"D?% +8 "core" Ŀ%^KOO ! +2 ."Plunge"Plunge.seek :locked +3 "valve0"Dz'Z +8 "syringe1"C=% +8 "core" ý%݊V +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C)'6 +78 "core" ' +26 é' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F.% +6 "core" Ʈ%Z[jQ  +7 #"detached from bill"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +3 "Cartridge" \ :D H  +7 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" y +3 "cartridge" y&:&: +4 "Cartridge" y  +5 "cartridge" y @?+0J59951 A+0C@ +3 #"detached from bill"Mounted lysis Cartridge board#J59951 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +49 "valve0"DK% +6 "core" %ZGO' J +3 "syringe1"CH& +8 "core" &O +3 "syringe1"CF% +4 "core" %6ɿS +3 "carousel0"F:% +3 "core" ƺ%[P[ \ +14 "carousel0"FL% +4 "core" %[|P9 \ +121 ."PROCESSING"SPR.command "read_sequences" +18 .SPR.command "stats_on" +10 "carousel0"FA% +3 "core" %[|PQ \ +6 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26aug0123h.spr.bz2 +197 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +49 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CS% +7 "core" %6N +44 "syringe1"CU% +3 "core" %6ɻS +47 "syringe1"CM% +3 "core" %6O +47 "syringe1"CO% +4 "core" %6N +46 "syringe1"C% +4 "core" Ç%6ɽU +46 "syringe1"C% +3 "core" È%6L +48 "syringe1"C(% +6 "core" è%6遝 +43 "syringe1"C3% +4 "core" ó%6J +47 "syringe1"C5% +3 "core" õ%6F +47 "syringe1"C% +3 "core" Ï%6ɼH +47 "syringe1"C % +3 "core" É%6D +47 "syringe1"C-% +3 "core" í%6G +47 "syringe1"Cy% +3 "core" %6G +49 "syringe1"C% +5 "core" Ñ%6ɼL +7 "Can"mX,jyu'#) +2 #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +3 .PinPort.in :locking +2 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +7 "valve1"E0( N +81 "core" Ű( +4 ."posArchive"Archive.in :fast +8 ."posLyS2"Lysis2.in :fast +5 "syringe0"B?d PZ<2 +4 ."PROCESSING"Lysis1.seek 950uL +4 "syringe0"B>d P\\k<2 +6 "syringe1"C2( +4 ."posArchive"Archive.seek :empty +11 ."posLyS2"Lysis2.seek 950uL +8 "syringe0"B( +5 B8' +335 "core" ò( +165 ¸' +328 ’( +2 ."PROCESSING"delayUntil "23:59:15.95" +2065 "Can"jX,kyu:# +11967 lX,lyu: $# +11972 kX,hyu6UZ$ +11970 lX,cyu7i$2 +11969 kX~,ayuA3$P +8301 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +7 B% +2 "core" †%{[u ~ +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 C & +3 "core" Ë&z^j v +57 ."Lysis2mixer"Lysis2.seek 700uL +4 ."PROCESSING"Lysis1.seek 700uL +6 "syringe0"BQ'| +3 "syringe1"C<(| +3486 "core" ' +6 ü( +2 ."PROCESSING"delay 5 seconds +79 "Can"kX,`yu:PT$n +423 ."PROCESSING"Handoff.select SPR +5 "valve0"D@ +48 ."PROCESSING"SPR.command "start 2 14" +170 #SPR stabilized +2 "syringe1"C"& +6 "core" â&|es~o +3 "syringe0"BB% +5 "core" %|e| +3 ."PROCESSING"delay 20 seconds @EDT1785643219.33 .SPR.command "start 2 0" +358 #Waiting up to 11:00 for SPR to enter state 1 +8945 "Can"kXj,eyu/$ +2863 ."PROCESSING"SPR.command "hold_on" +192 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B]% +7 "core" %|eN +5 "syringe1"CT& +4 "core" &|erN +49 ."Lysis2mixer"Lysis2.seek 400uL +5 "syringe0"BD' +5 ."PROCESSING"Lysis1.seek 400uL +7 "syringe1"C~( +4173 "core" ' +16 ( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +379 #Waiting up to 1:00:00 for SPR to enter state 7 +2758 "Can"kX7,lyt/ %$ +11972 lXb,kyu,)$ +11970 lX,iyu.-2$ +11972 lY",cyu&W0% +11973 lYR,`yu.46%" +11971 lYS,`yt*b7%@ +11970 kY2,byu.;b%^ +11970 lX,cyu0>%| +10949 #"SPRlogger"SPRsummary:1.28RIU,97.1RIU,85.5RIU,117RIU after 1927s +1022 "Can"lX,myu$zBu% +11971 lXW,oyu $F% +11970 jX,nyu $NJ% +11969 kW,oyu,UO% +11970 lX&,pyu\ $]R& +11968 kX,yyu#V&0 +11971 kYb,zyu+5Z&N +11974 jY,qyu,^$&l +876 "syringe1"Cc& +3 "core" &K% 0 +3 "syringe0"Be% +6 "core" %L3P +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +8770 "Can"jY,kyu.a& +2997 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Bh% +5 "core" %L,g  +4 "syringe1"CV& +8 "core" &K 2 +59 ."Lysis2mixer"Lysis2.seek 100uL +4 ."PROCESSING"Lysis1.seek 100uL +6 "syringe0"B$'ނ +8 "syringe1"C%(ނ +4180 "core" ¤' +6 å( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +373 #Waiting up to 1:00:00 for SPR to enter state 7 +2623 "Can"lYN,lyt.i& +11968 kY,lyu.ms& +11968 lX,fyu*p& +11970 kXz,\\yu.to' +11973 lX4,\\yu/w' +12021 mX,Yyt,%{'> +11924 jXq,Vyu.'\\ +11974 kY,Pyu/'z +11974 lZ>,Oyu $0' +11971 jZ,Gyu$q' +11970 kZ,Cyu #' +11973 jY,Cyu+l' +11976 kY,>yu $Nx( +11975 lY[,<` F 22 +7 F% +3 "core" Ƙ%[~P} \ +4 ."detached from bill"Carousel.home.seek 22 +3 "carousel0"F\ ' +16 "core" Ɗ' +2 "carousel0"FI% +5 "core" %V[Q@  +3 "syringe1"CP& +4 "core" &ނ2 +3 ."detached from bill"Plunge.seek :locked +3 "valve0"D'Z +8 "core" ĕ' +2 "syringe0"B& +5 "core" ‚&) +4 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +5 "syringe1"C{% +7 ."Archive"Archive.seek :clear +4 "syringe0"BG(j +4 "core" %6ɿG +7 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +5 CE& +8 ."Air"Air.seek :clear +2 "syringe1"C' +7 "core" &ނ2 +5 ."Lysis1"Lysis1.in :unlocking +4 "syringe0"B[% +2 "syringe1"C? P[(2 +61 "core" %ނ3d +4 ."Lysis1"Lysis1.seek :clear +9 "syringe1"Cj( +6 ."Lysis2"Lysis2.in :unlocking +4 "syringe0"B> P\\k(2 +6 "core" Ö' +3 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Br'j +320 "core" ( +105 ( +18 ' +4 "sampler0"H'% +4 "core" ȧ%: +10 ."detached from bill"Gate.power :motor,:OFF +3 .Gate.power :analytic1,:OFF +4 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785647283.40EDT @EDT1785647283.61 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +351 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +12 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +33 "valve0"DP +4 Dm +3 "core"  +6 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +10 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +8 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +7 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"D`% +4 Db& +3 "core" %FO(  +5 "carousel0"Ff% +8 "syringe0"Bp& +4 "syringe1"C% +6 ."Plunge_home"Plunge.home.jog 0 +6 "syringe1"Ci& +8 "syringe0"BZ% +6 "valve1"E>& +3 "valve0"D&) +27 "core" &O% Ԃ +2 %L[Q  &k  + Ð%݋i + &E o + %٤.  + ž&2O -U Ħ) +9 "valve0"D9% +7 ."PinPort_home"PinPort.home.jog 0 +7 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Archive_home"Archive.home.jog 0 +7 ."Toroid_home"Toroid.home.jog 0 +5 ."Lysis2_home"Lysis2.home.jog 0 +3 ."Air_home"Air.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +4 "valve1"Ex* +4 "syringe1"Ca* +3 "syringe0"Bs* +10 "valve0"Dv* + "syringe0"B^) +4 "carousel0"F) +6 "syringe1"C,) +17 "core" Ĺ%JG O# + * + * + * + * ) + Ɵ) + ì) +2 "syringe0"B% +13 "syringe1"Ct& +2 "valve0"D|& +3 "valve1"E& +3 "syringe1"C}% +2 "syringe0"BN& +2 ."Plunge_home"Plunge.in :homing +6 "valve0"D> (2 +23 "core" “%u٣o  + &Bj  +2 &"O +2 ŗ&*3O  +2 %m  +2 &t/  +7 ."Plunge_home"Plunge.jog 1487 +9 ."Lysis2_home"Lysis2.in :homing +7 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +5 ."PinPort_home"PinPort.in :homing +4 ."Archive_home"Archive.in :homing +5 ."Lysis1_home"Lysis1.in :homing +4 "valve0"D/) +3 "syringe0"B> (2 +4 "syringe1"C> -2 +2 "valve0"D?2 +3 "valve1"E?2 +3 "syringe0"B? -2 +6 "syringe1"C? (2 +5 ."Lysis2_home"Lysis2.jog 1619 +13 "syringe0"Bg)S +7 ."Air_home"Air.jog 1433 +7 ."Toroid_home"Toroid.jog 1349 +4 ."Lysis1_home"Lysis1.jog 1604 +7 ."PinPort_home"PinPort.jog 1250 +5 ."Archive_home"Archive.jog 1618 +4 "syringe1"C!) +4 "valve0"D4*E +3 "syringe1"Cw*D +6 "valve1"EC* +3 "syringe0"B*R +36 "core" į) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"Dk' +25 "core" ' +52 ."Plunge_home"Plunge.in :default +12 "valve0"D> P ޤ-2 +4 "core" ) +2 á) + * + Ž* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +6 ."Air_home"Air.setRawPosition! 0 +4 ."Lysis2_home"Lysis2.setRawPosition! 50 +3 "syringe1"C82 +3 ."Archive_home"Archive.setRawPosition! 50 +7 "syringe1"C7 +2 "syringe0"B72 +6 B82 +6 ."Lysis1_home"Lysis1.seek :home +5 ."Air_home"Air.seek :home +4 ."Lysis2_home"Lysis2.seek :home +6 ."Archive_home"Archive.seek :home +4 "syringe1"C ( +5 C*' +2 "syringe0"B_' +5 Bl( +16 "core" * + ê' +4 ."Air_home"Air.in :default +4 ."PinPort_home"PinPort.setRawPosition! 3150 +3 "syringe1"C>d P]z<2 +3 "valve1"E8 N +8 ."PinPort_home"PinPort.seek :home +3 "valve1"EX( +13 "core" Í( + ' ( +4 ."Lysis1_home"Lysis1.in :default +4 ."Archive_home"Archive.in :default +4 "syringe1"C?d P[<2 +3 ."Lysis2_home"Lysis2.in :default +4 "syringe0"B?d PZ<2 +3 B>d P\\k<2 +144 "core" Ĵ* +2 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +4 "valve0"D ( +5 "core" Ġ( +6 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +231 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"E& +8 "valve0"D( + "core" Ń&O  +8 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +2 "valve1"Ed( +42 "core" ( +294 ā( +3 ."detached from bill"Air.seek :clear +4 "syringe1"C1' +7 "core" ñ' +4 ."detached from bill"Lysis1.seek :clear +2 "syringe1"CW( +70 "core" ( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B6'j +77 "core" ¶' +2 ."detached from bill"Archive.seek :clear +3 "syringe0"B\\(j +77 "core" ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +7 F+% +3 "core" ƫ%X[Q  +4 ."detached from bill"Carousel.home.seek 22 +3 "carousel0"F' +16 "core" ƅ' +2 "valve0"DR% +4 "core" %^gO0 ! +4 #"detached from bill"Selecting Cartridge 20 +2 "valve0"D@% +7 "core" %^O* ! +2 "syringe1"C& +7 "core" Ý&H] +3 "syringe1"CY% +5 "core" %ݓY +2 "valve1"E& +6 "core" Ş&|O-  +2 ."detached from bill"Carousel.seek 20 +3 "carousel0"F7' +65 "core" Ʒ' +4 "carousel0"F?% +4 "core" ƿ%(S`S 2 +3 ."detached from bill"Carousel.reconfigure :align + "carousel0"F#` F 22 +7 "syringe1"C=& +4 "core" ý&JW +3 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +7 ."detached from bill"Lysis1.seek :locked +5 "syringe1"Cq( +248 "core" ( +3 "valve0"D.% +8 "core" Į%^OS ! +2 ."Plunge"Plunge.seek :locked +3 "valve0"Dn'Z +7 "syringe1"Cu% +7 "core" %ݔSt +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"C'6 +80 "core" ' +25 Ü' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F% +3 "core" ƛ%TSPpP  +6 #"detached from bill"Powering cartridge bus +265 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +5 "Cartridge" \ :D H  +9 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" Ky +3 "cartridge" yՎՎ +4 "Cartridge" Hy  +4 "cartridge" y /J59971 Z/MC% +4 #"detached from bill"Mounted lysis Cartridge board#J59971 +11 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"DF% +10 "core" %ZG#O 9 +47 "syringe1"C:& +6 "core" ú&R +2 "syringe1"CL% +4 "core" %64Q  +3 "carousel0"FA% +3 "core" %SOP \  +13 "carousel0"FS% +7 "core" %SOP \  +121 ."PROCESSING"SPR.command "read_sequences" +33 .SPR.command "stats_on" +10 "carousel0"FU% +3 "core" %SOPl \  +6 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26aug0201h.spr.bz2 +249 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +5 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"CM% +6 "core" %6)P ) +45 "syringe1"CO% +3 "core" %6I 3 +47 "syringe1"C% +3 "core" Ç%6)N ) +47 "syringe1"C% +5 "core" È%6$L . +45 "syringe1"C(% +3 "core" è%6=O  +47 "syringe1"C3% +3 "core" ó%6*L ( +47 "syringe1"C5% +3 "core" õ%6-M % +47 "syringe1"C% +3 "core" Ï%6#L / +48 "syringe1"C % +3 "core" É%6(K * +47 "syringe1"C-% +3 "core" í%6(I * +47 "syringe1"Cy% +10 "core" %6+ ' +40 "syringe1"C% +3 "core" Ñ%6%K - +48 "syringe1"C% +3 "core" %6.ā $ +46 "syringe1"C % +4 "core" Ì%6.K $ +4 "Can"kX,d P\\k<2 +4 ."posArchive"Archive.seek :empty +11 ."PROCESSING"Lysis1.seek 950uL +5 ."posLyS2"Lysis2.seek 950uL +7 "syringe0"Bo( +2 "syringe1"C( +7 "syringe0"B ' +357 "core" Æ( +149 ‹' +320 ( +2 ."PROCESSING"delayUntil "01:18:44.08" +4157 "Can"kX,Byu?FP( +11973 kY,Jyu;( +11972 jZ,Oyu=?( +11971 kZ,Pyu<Y( +11973 mY,Qyu=  ) +6251 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +7 B8% +3 "core" ¸%{> x +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +7 C2& +3 "core" ò&z< +58 ."Lysis2mixer"Lysis2.seek 700uL +4 ."PROCESSING"Lysis1.seek 700uL +5 "syringe0"B"'| +4 "syringe1"CB(| +3486 "core" ¢' +4 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +4 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +121 #SPR stabilized +2 "syringe1"C]& +6 "core" &|ex^u +4 "syringe0"BT% +6 "core" %|e_ +3 ."PROCESSING"delay 20 seconds +1432 "Can"lYB,Syu1/) +570 ."PROCESSING"SPR.command "start 2 0" +313 #Waiting up to 11:00 for SPR to enter state 1 +11090 "Can"lX,Tyu0~4)< +718 ."PROCESSING"SPR.command "hold_on" +139 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BQ% +7 "core" %|eb +5 "syringe1"C<& +5 "core" ü&|e}g +57 ."Lysis2mixer"Lysis2.seek 400uL +3 ."PROCESSING"Lysis1.seek 400uL +6 "syringe0"Bc' +3 "syringe1"Ce( +4181 "core" ' +6 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +338 #Waiting up to 1:00:00 for SPR to enter state 7 +4998 "Can"lX,]yt/ă)Z +11972 jXQ,Yyu)")x +11972 kX,Tyu/ː) +11970 kXO,Wyu-) +11971 jY,Vyu/ҏ) +11971 iY,Vyu0:) +11970 kY,Ryu-ٷ* +11966 jY<,Pyu/4*, +8809 #"SPRlogger"SPRsummary:2.64RIU,27.5RIU,14.2RIU,28.2RIU after 1927s +3159 "Can"jX,Nyu$4*J +11972 kX,Jyu$y#*h +11969 kX`,Eyu #]* +11971 jX1,Byu&EK* +11971 jX,@yu#(* +11970 jYg,?yu./Z* +11969 jY,=yu** +10709 "syringe1"Ch& +4 "core" &Kc 0 +3 "syringe0"BV% +7 "core" %L@< +4 ."PROCESSING"delay 20 seconds +1243 "Can"jY,;yu13_+ +759 ."PROCESSING"SPR.command "start 2 0" +306 #Waiting up to 11:00 for SPR to enter state 1 +10906 "Can"kY@,5yu.+: +862 ."PROCESSING"SPR.command "hold_on" +189 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BD% +7 "core" %L<  +3 "syringe1"C~& +6 "core" &K 7 +54 ."Lysis2mixer"Lysis2.seek 100uL +5 ."PROCESSING"Lysis1.seek 100uL +7 "syringe0"B;'ނ +3 "syringe1"C(ނ +4184 "core" »' +4 Ô( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +326 #Waiting up to 1:00:00 for SPR to enter state 7 +4812 "Can"lX,=yt.q+X +11969 iX6,;yu(C +v +11969 kX,3yu."+ +11968 jX,5yu/+ +11972 jX,5yu0+ +11972 jYZ,3yu,+ +11972 jZ ,6yu0G, +11972 kY,7yu. ,* +11970 kY,>yu $$y,H +11972 lXz,?yu $ (,f +11971 kX ,@yu$,, +11974 iW,?yu00, +11975 jW,?yu #4, +11975 iX),Byu(J8, +11970 lY,Fyu.<` F 22 +7 F% +3 "core" Ƃ%SNYPN \  +4 ."detached from bill"Carousel.home.seek 20 +3 "carousel0"F{' +19 "core" ' +2 "carousel0"FE% +4 "core" %1S\\T 2 +3 "syringe1"C[& +5 "core" &ނ2m +3 ."detached from bill"Plunge.seek :locked +2 "valve0"D0'Z +8 "core" İ' +3 "syringe0"B& +4 "core" „&)j +5 "syringe1"Cz% +3 ."Archive"Archive.in :unlocking +5 "syringe0"B? PZ-2 +6 ."Archive"Archive.seek :clear +2 "syringe0"B(j +7 "core" %6a B +4 ."Air"Air.in :unlocking +3 "syringe1"C> P]z-2 +6 C)& +6 ."Air"Air.seek :clear +3 "syringe1"C%' +6 "core" é&ނ2 +7 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +5 "syringe0"B$% +9 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C'( +6 "core" ¤%ނ3s; +5 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +6 ."Lysis2"Lysis2.seek :clear +3 "syringe0"Bm'j +39 "core" å' +340 ’( +43 ç( +21 ' +3 "sampler0"H`% +5 "core" %5 +10 ."detached from bill"Gate.power :motor,:OFF +53 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +5 #Safely stopped and ready to power off @1785652049.54EDT @EDT1785652049.76 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +162 "Can"kY:,Iyu@!- +186 ."detached from bill"Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +13 #Powering core bus +236 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +31 "valve0"DP +4 D> +3 "core" ľ +8 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +9 ."detached from bill"PhaseSeparator.configure :default +3 "carousel1"G$ +8 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +8 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +5 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +9 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +7 #"detached from bill"All elf controllers running firmware version 4.94 +4 .Gate.power :motor,:ON,limit to 5000mA +7 "valve0"DZ% +6 "core" %FO(  +2 "carousel0"Fi% +4 "valve0"D& +4 "syringe0"Bp& +5 "syringe1"Cf% +4 Cb& +11 "syringe0"B9% +4 ."Plunge_home"Plunge.home.jog 0 +6 "valve1"E& +3 "valve0"Dt) +34 "core" %MSOQ H + Đ&CO& Y + &'m  + %݈o + &O, e ¹%٨  + œ&O - + ) +10 "valve0"D}% +8 ."PinPort_home"PinPort.home.jog 0 +5 ."Lysis1_home"Lysis1.home.jog 0 +4 ."Carousel_home"Carousel.home.jog 0 +5 ."Toroid_home"Toroid.home.jog 0 +5 ."Air_home"Air.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +6 ."Lysis2_home"Lysis2.home.jog 0 +4 "valve1"EN* +3 "carousel0"F) +3 "syringe1"C|* +6 "valve0"D* +5 "syringe1"CJ) + "syringe0"Bj* +4 BG) +26 "core" %JFO"  + * + * + Ɨ) ĕ* + ) + * + ) +2 "syringe0"B% +8 "syringe1"C% +3 "valve1"E\ & +2 "syringe1"Cr& +6 "valve0"D&& +2 ."Plunge_home"Plunge.in :homing +25 "syringe0"B^& +2 "valve0"D> (2 +13 ."Plunge_home"Plunge.jog 1492 +3 "valve0"D,) +6 "core" ™%u٪m  + Ö%݄J + Ŋ&#O + &Ul + Ħ&HO &sn  +8 ."PinPort_home"PinPort.in :homing +8 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +5 ."Toroid_home"Toroid.in :homing +4 ."Lysis1_home"Lysis1.in :homing +5 "valve1"E?2 +3 ."Archive_home"Archive.in :homing +6 "syringe0"B> (2 +3 "syringe1"C> -2 +2 "valve0"D?2 +4 "syringe1"C? (2 +3 "syringe0"B? -2 +4 ."PinPort_home"PinPort.jog 1242 +11 ."Lysis2_home"Lysis2.jog 1617 +7 ."Lysis1_home"Lysis1.jog 1601 +11 ."Air_home"Air.jog 1432 +4 ."Toroid_home"Toroid.jog 1354 +5 "valve1"Ev* +4 ."Archive_home"Archive.jog 1620 +5 "syringe1"Ca*A +3 "syringe0"Bx)Q +5 "syringe1"Cs) +2 "valve0"D/*J +4 "syringe0"Bw*T +19 "core" Ĭ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +6 ."Plunge_home"Plunge.seek :home +3 "valve0"D!' +26 "core" ġ' +3 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +49 "core" * +2 ."Lysis1_home"Lysis1.setRawPosition! 50 +3 "syringe1"C82 +6 "core" ) + ) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +6 ."Air_home"Air.setRawPosition! 0 +3 "syringe0"B72 +4 ."Lysis1_home"Lysis1.seek :home +5 "syringe1"C7 +6 ."Lysis2_home"Lysis2.seek :home +5 "syringe1"Cg( +2 ."Air_home"Air.seek :home +8 "syringe0"B' +4 "syringe1"CC' +59 "core" * + * + ' + ( Ž' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +19 ."Lysis2_home"Lysis2.in :default +3 ."Lysis1_home"Lysis1.in :default +5 ."Air_home"Air.in :default +5 ."Archive_home"Archive.setRawPosition! 50 +3 "valve1"E8 N +3 "syringe0"B>d P\\k<2 +5 "syringe1"C?d P[<2 +8 C>d P]z<2 + "syringe0"B82 + ."PinPort_home"PinPort.seek :home +11 "valve1"E( +6 ."Archive_home"Archive.seek :home +4 "syringe0"B4( +27 "core" ´( +3 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +109 "core" į* +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +6 ."Toroid_home"Toroid.seek :home +5 "valve0"D ( +4 "core" Ġ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +259 "core" Ń( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +4 "valve1"EX& +4 "valve0"D+( +5 "core" &O +7 ."detached from bill"PinPort.in :unlocking + "valve1"E? B2 +8 ."detached from bill"PinPort.seek :clear +3 "valve1"ER( +42 "core" ( +288 ī( +3 ."detached from bill"Air.seek :clear +3 "syringe1"C@' +7 "core" ' +3 ."detached from bill"Lysis1.seek :clear +3 "syringe1"C*( +69 "core" ê( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B'j +76 "core" ' +3 ."detached from bill"Archive.seek :clear +2 "syringe0"B (j +77 "core" ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +8 FY% +3 "core" %RSN8P  +4 ."detached from bill"Carousel.home.seek 20 +2 "carousel0"F' +20 "core" ƞ' +3 "valve0"D_% +4 "core" %^O/ !z +4 #"detached from bill"Selecting Cartridge 14 +3 "valve0"D?% +11 "core" Ŀ%^O0 !z +2 "syringe1"C=& +5 "core" ý&fW +3 "syringe1"Cl% +4 "core" %݊W +3 "valve1"E& +4 "core" ş&=O+ 7 +4 ."detached from bill"Carousel.seek 14 +3 "carousel0"F' +148 "core" ƅ' +5 "carousel0"F.% +3 "core" Ʈ% '9S 2 +7 ."detached from bill"Carousel.reconfigure :align +3 "carousel0"F#` F 22 +7 "syringe1"Cu& +3 "core" &WY +4 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +5 "core" ƅ' N9!Q  +2 !o:Slide::Warning : mesg"2Storage Carousel (SC) reseek at 14 + 4 counts: @replyo:I2C::Servo::AbsMove3Reply : @error: reseek:@destinationi%: @channeli: @statuso:I2C::Servo::Status: @pwmi:@deltaPressiQ:@pastThresholdF: @voltagef15.019179185076093T: @currentiS: @type2F:@outPressi!:\ @lostF: @inPressi9:@velocityi: @pastFLSF:\ @homeF: @enabledF: @erri:@positioni : @pastRLSF: @tagi\ : @sourceiK:@axisName"Storage Carousel (SC):bt["3/home/esp/esp2/lib/slide.rb:40:in `initialize'"3/home/esp/esp2/lib/i2c/servo.rb:1255:in `call'"6/home/esp/esp2/lib/i2c/servo.rb:1255:in `deliver'"3/home/esp/esp2/lib/i2c/msg.rb:610:in `process'"2/home/esp/esp2/lib/msg.rb:220:in `initialize'",/home/esp/esp2/lib/msg.rb:217:in `loop'"2/home/esp/esp2/lib/msg.rb:217:in `initialize'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 + ƅ' +2 "syringe1"CW( +251 "core" ( +3 "valve0"D% +8 "core" ě%^}O| ! +2 ."Plunge"Plunge.seek :locked +3 "valve0"Dk'Z +8 "syringe1"C1% +7 "core" ñ%ݏP +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +6 ."Air"Air.seek :locked +3 "syringe1"CK'6 +79 "core" ' +25 ' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 FH% +3 "core" % U9pP  +7 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" Fy +3 "cartridge" yGBGB +3 "Cartridge" :y  +4 "cartridge" y _S&0J59913 U&0(CJ +3 #"detached from bill"Mounted lysis Cartridge board#J59913 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"DL% +5 "core" %ZGO/ X +3 "syringe1"CA& +6 "core" &聸 +5 "syringe1"CS% +4 "core" %6P +3 "carousel0"FU% +3 "core" % 9aPX \ +4 "carousel0"FM% +6 "core" % 9`PX \ +119 ."PROCESSING"SPR.command "read_sequences" +60 .SPR.command "stats_on" +95 "carousel0"FO% +4 "core" % 9P{ \ +5 ."PROCESSING"SPR.command "start 0 1" +70 ."SPRlogger"SPR.start /var/log/esp/spr26aug0202h.spr.bz2 +258 #"PROCESSING"Waiting for baseline SPR stabilization + .Handoff.select :waste +5 "valve0"D +50 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C% +6 "core" Ç%6Q +44 "syringe1"C% +3 "core" È%6ɼL +47 "syringe1"C(% +4 "core" è%6K +46 "syringe1"C3% +5 "core" ó%6 +45 "syringe1"C5% +3 "core" õ%6I +47 "syringe1"C% +3 "core" Ï%6N +47 "syringe1"C % +3 "core" É%6O +47 "syringe1"C-% +4 "core" í%6ɾJ +47 "syringe1"Cy% +3 "core" %6ɴJ +47 "syringe1"C% +3 "core" Ñ%6ɿK +47 "syringe1"C% +5 "core" %6L +45 "syringe1"C % +3 "core" Ì%6F +48 "syringe1"Cd% +3 "core" %6ɾJ +46 "syringe1"C\\% +4 "core" %6L +4 "Can"kY:,Iyu&A-& +2 #"PROCESSING"Syringe tare is 51% of full scale at 23C (drifted -0%) +3 .PinPort.in :locking +4 "valve1"E? B22 +7 ."PROCESSING"PinPort.seek :locked +2 "valve1"Eq( N +81 "core" ( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +7 ."posLyS2"Lysis2.in :fast +6 ."PROCESSING"Lysis1.seek 950uL +7 "syringe0"B>d P\\k<2 +4 ."posArchive"Archive.seek :empty +11 "syringe1"C6( +4 ."posLyS2"Lysis2.seek 950uL +6 "syringe0"B8( +4 B2' +347 "core" ö( +155 ²' +329 ¸( +2 ."PROCESSING"delayUntil "02:38:12.11" +6193 "Can"kYS,Pyu;EDZ-8 +11975 jYa,Oyu8yG-V +11973 lYL,Pyu;<J-t +11970 lY,Syu6wM- +11973 mX,^yuBQ$- +4207 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +8 B % +2 "core" ‹%z +3 ."PROCESSING"Lysis1.in :DAmix +2 "syringe1"C?d2 P[<2 +7 C& +3 "core" Æ&z r +57 ."Lysis2mixer"Lysis2.seek 700uL +8 ."PROCESSING"Lysis1.seek 700uL +3 "syringe0"B]'| +3 "syringe1"CT(| +3487 "core" ' +4 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +121 #SPR stabilized + "syringe1"CQ& +6 "core" &|e{ +3 "syringe0"B<% +6 "core" ¼%|e +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +262 #Waiting up to 11:00 for SPR to enter state 1 +1220 "Can"lX,cyu0V- +10547 ."PROCESSING"SPR.command "hold_on" +140 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +2 "syringe0"B"% +7 "core" ¢%|ey +5 "syringe1"CB& +6 "core" &|e}t +52 ."Lysis2mixer"Lysis2.seek 400uL +6 "syringe0"Bh' +8 ."PROCESSING"Lysis1.seek 400uL +3 "syringe1"CV( +1191 "Can"iXB,dyst[W- +2982 "core" ' +10 ( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +288 #Waiting up to 1:00:00 for SPR to enter state 7 +7185 "Can"lW,ryu/5b.\ +11971 lW,qyu/f.( +11973 kWo,pyu.i.F +11973 lWo,uyu0m.d +11975 jX ,qyu2p. +11972 iX,oyu%tC. +11966 kX,nyu/w. +11968 iX,kyu0{>. +6575 #"SPRlogger"SPRsummary:2.61RIU,10.7RIU,-2.28RIU,9.27RIU after 1925s +5395 "Can"lY,cyu $. +11968 mY,gyu $ P/ +11967 jX,jyu $/6 +11967 kX,hyu/W/T +11968 jX,kyu\ $T/r +11967 lX,fyu/e/ +11965 kX,eyu,/ +8496 "syringe1"CD& +6 "core" &Km * +2 "syringe0"B~% +6 "core" %L   +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +303 #Waiting up to 11:00 for SPR to enter state 1 +1148 "Can"iX,eyu3h/ +10619 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Bc% +6 "core" %L%W  +4 "syringe1"Ce& +4 "core" &K\\ $ +56 ."Lysis2mixer"Lysis2.seek 100uL +6 ."PROCESSING"Lysis1.seek 100uL +7 "syringe0"B#'ނ +2 "syringe1"C(ނ +1125 "Can"hX,_ys!tE/ +3057 "core" £' +4 Ø( +2 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +287 #Waiting up to 1:00:00 for SPR to enter state 7 +7119 "Can"kX,hyu-00 +11968 jX,hyu00& +11969 lX,byu*)0D +11969 jX,gyu-0b +11970 lX,jyu);$0 +11968 kX,lyu.0 +11967 lX,lyu.O0 +11968 iX,pyu-0 +11967 jXn,syu $.¤0 +11965 lX|,wyu $?1 +11967 kX,oyu #14 +11968 jX,kyu/1R +11969 kY\ ,eyu\ $ 1p +11967 lY,cyu-01 +11969 lY),cyu*t1 +8578 #"PROCESSING"SPR analysis completed +66 #"SPRlogger"Ended log spr26aug0202h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +97 "carousel0"FI% +3 "core" % 9oP_ \ +6 "sampler0"H% +4 "core" Ȃ%! +5 ."closeHandoff"Handoff.select :close +3 "valve0"D +51 ."detached from bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"EE& +5 "core" & N;O8 +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +9 ."detached from bill"PinPort.seek :clear +3 "valve1"E[( +81 "core" ( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F% +3 "core" Ƅ% 9Pl \ +4 ."detached from bill"Carousel.home.seek 14 +3 "carousel0"Fz' +17 "core" ' +2 "carousel0"F)% +4 "core" Ʃ% L9sQ  +3 "syringe1"C$& +4 "core" ä&ނ3` +4 ."detached from bill"Plunge.seek :locked +3 "valve0"D'Z +7 "core" Ě' +3 "syringe0"B7& +4 "core" ·&)b +5 ."Archive"Archive.in :unlocking +4 "syringe1"Cn% +3 "syringe0"B? PZ-2 +6 "core" %6g ~ +3 ."Archive"Archive.seek :clear +4 "syringe0"Bo(j +4 "syringe1"C& +6 ."Air"Air.in :unlocking +11 "syringe1"C> P]z-2 +5 "core" Ü&ނ3XZ +4 ."Air"Air.seek :clear +8 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B% +4 "syringe1"C;' +2 C? P[(2 +10 ."Lysis1"Lysis1.seek :clear +8 "syringe1"C`( +4 "core" ”%ނ3c +4 ."Lysis2"Lysis2.in :unlocking +3 "syringe0"B> P\\k(2 +12 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B>'j +51 "core" û' +322 ( +43 ( +32 ¾' +4 "sampler0"HZ% +3 "core" %4 +11 ."detached from bill"Gate.power :motor,:OFF +2 .Gate.power :analytic1,:OFF +5 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785656815.40EDT @EDT1785656815.59 .Gate.power :sampler,:OFF,limit to 3500mA +2 .Gate.power :skimmer,:OFF,limit to 3500mA +354 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +69 #Powering core bus +176 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +93 "valve0"DP +3 D +3 "core" ē +6 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +9 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +9 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +9 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +8 ."detached from bill"PhaseSeparator.configure :default +2 "carousel1"G$ +9 ."detached from bill"Archive.configure :default + "syringe0"B$d PZ<2 +10 ."detached from bill"Air.configure :default +2 "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +8 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +7 #"detached from bill"All elf controllers running firmware version 4.94 +5 .Gate.power :motor,:ON,limit to 5000mA +10 "valve0"D9% +2 Db& +4 "core" Ĺ%FO&  +6 "carousel0"Ff% +6 "syringe0"Bi& +6 ."Plunge_home"Plunge.home.jog 0 +6 "syringe1"C% +12 Cp& +3 "syringe0"B}% +6 "valve0"D\ ) +3 "valve1"E& +21 "core" &#YO y + %L9P  + & h  + Ð%݆m + &Pm d + %ٔ/  + ř&*MN , Ċ) +8 "valve0"Dr% +7 ."PinPort_home"PinPort.home.jog 0 +7 ."Lysis1_home"Lysis1.home.jog 0 +5 ."Archive_home"Archive.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +6 ."Carousel_home"Carousel.home.jog 0 +4 "valve1"E* +3 "syringe1"C^* +2 "syringe0"B&* +10 "valve0"D0* +4 "syringe0"BP) +4 "syringe1"C') + "carousel0"F{) +24 "core" %JGO* + Ŗ* + * + ¦* + İ* +2 ) +2 ç) +2 ) +3 "syringe0"B% +9 "syringe1"C% +4 C%& +3 "valve1"Em& +2 "valve0"Dt& +4 "syringe0"BN& +7 ."Plunge_home"Plunge.in :homing +14 "valve0"D> (2 +13 "core" %rٟj + Ò%݃2 + å&Sj + &!O  + &2O + &pi  +10 ."Plunge_home"Plunge.jog 1486 +10 ."PinPort_home"PinPort.in :homing +5 ."Lysis1_home"Lysis1.in :homing +5 ."Toroid_home"Toroid.in :homing +4 ."Lysis2_home"Lysis2.in :homing +5 ."Air_home"Air.in :homing +4 ."Archive_home"Archive.in :homing +5 "valve0"DG) +3 "valve1"E?2 +5 "syringe1"C? (2 +4 "valve0"D?2 +3 "syringe0"B> (2 + "syringe1"C> -2 +5 "syringe0"B? -2 + ."PinPort_home"PinPort.jog 1241 +5 ."Lysis1_home"Lysis1.jog 1602 +10 "valve1"E|* +5 "syringe1"CJ*B +10 ."Air_home"Air.jog 1432 +5 ."Toroid_home"Toroid.jog 1351 +4 ."Lysis2_home"Lysis2.jog 1620 +8 ."Archive_home"Archive.jog 1622 +8 "syringe1"C) +2 "valve0"Dj*G +3 "syringe0"B,)T +7 Ba*V +36 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 +2 "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"Dx' +25 "core" ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +6 "core" * +4 ."Lysis1_home"Lysis1.setRawPosition! 50 + "syringe1"C82 +6 ."Lysis1_home"Lysis1.seek :home +2 "syringe1"Cs( +9 "core" Õ) +2 ."Air_home"Air.setRawPosition! 0 +2 "syringe1"C7 +5 ."Air_home"Air.seek :home +3 "syringe1"Cv' +10 "core" ( + ' + ¬) + * +2 ."Lysis2_home"Lysis2.setRawPosition! 50 +5 ."Lysis1_home"Lysis1.in :default +4 ."Archive_home"Archive.setRawPosition! 50 +4 ."Air_home"Air.in :default +5 "syringe0"B72 +3 "syringe1"C?d P[<2 +5 "syringe0"B82 +2 "syringe1"C>d P]z<2 +3 ."Lysis2_home"Lysis2.seek :home +8 "syringe0"Bw' +10 ."Archive_home"Archive.seek :home +6 "syringe0"B/( +59 "core" * + ' +2 ."PinPort_home"PinPort.setRawPosition! 3150 +12 ."Lysis2_home"Lysis2.in :default +4 "valve1"E8 N +3 "syringe0"B>d P\\k<2 +8 ."PinPort_home"PinPort.seek :home +4 "valve1"EX( +8 "core" ¯( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +150 "core" * +3 ."Toroid_home"Toroid.setRawPosition! 0 +3 "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +4 "core" Ġ( +3 ."Toroid_home"Toroid.in :default +2 "valve0"D? ֜2 +247 "core" ( +2 ."PinPort_home"PinPort.in :default +2 "valve1"E? B22 +35 ."clearToroid"Toroid.seek :clear +3 "valve1"E& +4 "valve0"DY( +7 "core" Ń&O  +5 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +4 "valve1"E_( +43 "core" ( +293 ( +3 ."detached from bill"Air.seek :clear +5 "syringe1"C?' +2 "Can"kY,,iyu #L1 +6 "core" ÿ' +3 ."detached from bill"Lysis1.seek :clear +2 "syringe1"CC( +70 "core" ( +2 ."detached from bill"Lysis2.seek :clear +3 "syringe0"Bg'j +77 "core" ' +3 ."detached from bill"Archive.seek :clear +3 "syringe0"B=(j +76 "core" ½( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 Fl% +3 "core" % Y9Q'  +7 ."detached from bill"Carousel.home.seek 14 +5 "carousel0"F' +14 "core" Ɵ' +3 "valve0"D% +5 "core" Ď%^O0 !w +3 #"detached from bill"Selecting Cartridge 13 +2 "valve0"D4% +9 "core" Ĵ%^sO6 ! +2 "syringe1"C.& +5 "core" î&_X +2 "syringe1"Cu% +4 "core" %݌W +3 "valve1"E& +6 "core" ŗ&O5 [ +3 ."detached from bill"Carousel.seek 13 +3 "carousel0"F' +45 "core" ƞ' +4 "carousel0"F% +4 "core" ƛ% *5US 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C1& +4 "core" ñ&\\X +3 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +8 ."detached from bill"Lysis1.seek :locked +3 "syringe1"C*( +250 "core" ê( +3 "valve0"DH% +6 "core" %^|OQ ! +3 ."Plunge"Plunge.seek :locked +2 "valve0"D!'Z +9 "syringe1"C@% +7 "core" %ݏS +3 ."Air"Air.in :fast + "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"CF'6 +77 "core" ġ' +27 ' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 F:% +4 "core" ƺ% W5QcP  +7 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +4 ."detached from bill"Gate.setIR 1750,5000,0,6 +13 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +8 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +4 "Cartridge" Ly +3 "cartridge" yAA +4 "Cartridge" Ay  +4 "cartridge" y a"&0J59949 Y%&0lC/q +4 #"detached from bill"Mounted lysis Cartridge board#J59949 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +6 "valve0"DS% +3 "core" %ZFO- p +3 "syringe1"CU& +5 "core" &T +3 "syringe1"CM% +5 "core" %6T +2 "carousel0"FO% +5 "core" % 5RPq \  +3 "carousel0"F% +6 "core" Ƈ% 5RP] \  +116 ."PROCESSING"SPR.command "read_sequences" +36 .SPR.command "stats_on" +9 "carousel0"F% +4 "core" ƈ% 5QPv \  +5 ."PROCESSING"SPR.command "start 0 1" +71 ."SPRlogger"SPR.start /var/log/esp/spr26aug0203h.spr.bz2 +196 #"PROCESSING"Waiting for baseline SPR stabilization +58 .Handoff.select :waste +13 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C(% +6 "core" è%6Q +44 "syringe1"C3% +4 "core" ó%6W +46 "syringe1"C5% +3 "core" õ%6Q } +47 "syringe1"C% +5 "core" Ï%6M +45 "syringe1"C % +4 "core" É%6M +46 "syringe1"C-% +4 "core" í%6M +47 "syringe1"Cy% +5 "core" %6 +44 "syringe1"C% +4 "core" Ñ%6I  +47 "syringe1"C% +4 "core" %6J +46 "syringe1"C % +3 "core" Ì%6F +47 "syringe1"Cd% +4 "core" %6D +46 "syringe1"C\\% +3 "core" %6H +47 "syringe1"CR% +3 "core" %6D +47 "syringe1"CW% +4 "core" %6L +4 "Can"kY,,iyu&%1 +2 #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +5 .PinPort.in :locking +2 "valve1"E? B22 +5 ."PROCESSING"PinPort.seek :locked +3 "valve1"E ( N +83 "core" ō( +4 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +11 ."posLyS2"Lysis2.in :fast +7 ."PROCESSING"Lysis1.seek 950uL +4 ."posArchive"Archive.seek :empty +5 "syringe0"B>d P\\k<2 +4 "syringe1"C( +6 "syringe0"B ( +9 ."posLyS2"Lysis2.seek 950uL +6 "syringe0"B' +344 "core" Ý( +157 †' +317 ‹( +2 ."PROCESSING"delayUntil "03:57:35.18" +8558 "Can"lYH,fyu:]J1 +11997 iYK,gyu6~2 +11943 kYA,lyu:H2$ +11969 kYG,lyu:g2B +11966 kYi,jyu:)2` +1763 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +6 B2% +4 "core" ²%{0 +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +8 C6& +3 "core" ö&z클 u +58 ."Lysis2mixer"Lysis2.seek 700uL +5 ."PROCESSING"Lysis1.seek 700uL +9 "syringe0"BQ'| +2 "syringe1"C<(| +3486 "core" ' +4 ü( +2 ."PROCESSING"delay 5 seconds +502 .Handoff.select SPR +6 "valve0"D@ +47 ."PROCESSING"SPR.command "start 2 14" +115 #SPR stabilized +5 "syringe1"C"& +6 "core" â&|eyR +3 "syringe0"BB% +6 "core" %|eꁗ +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +258 #Waiting up to 11:00 for SPR to enter state 1 +3656 "Can"lY_,nyu/2~ +8111 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"B]% +9 "core" %|et +5 "syringe1"CT& +4 "core" &|es +53 ."Lysis2mixer"Lysis2.seek 400uL +7 ."PROCESSING"Lysis1.seek 400uL +5 "syringe0"BD' +3 "syringe1"C~( +3626 "Can"kY,lysvzv2 +557 "core" ' +4 ( +2 ."PROCESSING"delay 15 seconds +1503 .SPR.command "hold_off" +285 #Waiting up to 1:00:00 for SPR to enter state 7 +9615 "Can"lY_,ryu/2 +11966 mX,qyu-"2 +11965 kX3,pyu/2 +11965 lXD,oyu/ 3 +11967 kYi,vyu032 +11963 lZ,syu.I3P +11965 lZ-,tyu,3n +11968 mY,syu.F3 +4183 #"SPRlogger"SPRsummary:3.36RIU,9.99RIU,-3.38RIU,9.75RIU after 1924s +7783 "Can"kY_,pyu$OD3 +11968 lY ,myu#!3 +11968 lX,iyu #%3 +11968 lX,iyu+)a4 +11968 lX,kyu #-4" +11967 lX,lyu/1m4@ +11966 kW,jyu/44^ +6102 "syringe1"Cc& +5 "core" &KG , +3 "syringe0"Be% +5 "core" %L5 \ +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +308 #Waiting up to 11:00 for SPR to enter state 1 +3536 "Can"jW,gyu+8j4| +8231 ."PROCESSING"SPR.command "hold_on" +190 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"Bh% +6 "core" %L0  +5 "syringe1"CV& +8 "core" &KN! & +55 ."Lysis2mixer"Lysis2.seek 100uL +4 ."PROCESSING"Lysis1.seek 100uL +6 "syringe0"BI'ނ +6 "syringe1"C(ނ +3449 "Can"jX?,kysu*>4 +733 "core" ' +6 Â( +3 ."PROCESSING"delay 15 seconds +1502 .SPR.command "hold_off" +374 #Waiting up to 1:00:00 for SPR to enter state 7 +9347 "Can"kYo,oyu,D84 +11964 kY,oyu0G4 +11966 kY,lyu.K34 +11967 lY ,jyu0N5 +11968 mX,dyu&1R+50 +11964 jX,dyu.U5N +11966 jXP,byu.'YU5l +11966 jX ,cyu.\\5 +11965 lW,eyu$$`5 +11968 kW,hyu $Je 5 +11966 lW,jyu #i?5 +11961 lXQ,pyu,'l6 +11962 kY=,pyu $8q6 +11962 mYL,zyu-2t6> +11964 jY%,oyu0x{6\\ +6341 #"PROCESSING"SPR analysis completed +66 #"SPRlogger"Ended log spr26aug0203h.spr.bz2 +2 ."PROCESSING"SPR.command "stats_off" +93 "carousel0"FE% +3 "core" % 5PP[ \  +6 "sampler0"H% +5 "core" Ȅ%& +4 ."closeHandoff"Handoff.select :close +5 "valve0"D +50 ."detached from bill"Gate.power :heat,:OFF +2 .Gate.power :cartridge,:OFF +4 "valve1"E)& +7 "core" ũ& N;O3  +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +11 ."detached from bill"PinPort.seek :clear +3 "valve1"E$( +80 "core" Ť( +2 ."detached from bill"Carousel.in :default +2 "carousel0"F><` F 22 +7 F7% +3 "core" Ʒ% 5O PO \  +4 ."detached from bill"Carousel.home.seek 13 +3 "carousel0"Fn' +17 "core" ' +3 "carousel0"F% +5 "core" Ɯ% '5O}S 2 +2 "syringe1"C& +4 "core" Ô&ނ3  +3 ."detached from bill"Plunge.seek :locked +2 "valve0"Dq'Z +8 "core" ' +3 "syringe0"B& +4 "core" …&)~ +5 ."Archive"Archive.in :unlocking +3 "syringe1"Ck% +4 "syringe0"B? PZ-2 +7 "core" %6 +2 ."Archive"Archive.seek :clear +9 ."Air"Air.in :unlocking +4 "syringe0"B8(j +3 "syringe1"CK& +8 C> P]z-2 +8 ."Air"Air.seek :clear +4 "syringe1"C' +7 "core" &ނ3Ɓ +5 ."Lysis1"Lysis1.in :unlocking +3 "syringe0"B#% +4 "syringe1"C? P[(2 +7 "core" £%ނ3} +3 ."Lysis1"Lysis1.seek :clear +7 ."Lysis2"Lysis2.in :unlocking +5 "syringe1"CZ( +2 "syringe0"B> P\\k(2 +8 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B'j +41 "core" Ø' +349 ¸( +42 ( +10 “' +4 "sampler0"H9% +3 "core" ȹ%' +11 ."detached from bill"Gate.power :motor,:OFF +56 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +3 .Gate.power :main,:OFF +4 #Safely stopped and ready to power off @1785661579.84EDT @EDT1785661580.07 .Gate.power :sampler,:OFF,limit to 3500mA +3 .Gate.power :skimmer,:OFF,limit to 3500mA +346 .Gate.power :main,:ON,limit to 5000mA +3 .Gate.power :analytic1,:ON,limit to 4000mA +13 #Powering core bus +234 #"Probe-AM1"SPR Module 1, FW Rev. B, FPGA Rev. C +33 "valve0"DP +3 D} +3 "core"  +7 ."detached from bill"Plunge.configure :default +2 "valve0"D# P ޤ-2 +10 ."detached from bill"SamplePump.configure :default +2 "sampler0"H#^ #(22 +9 ."detached from bill"Toroid.configure :default +2 "valve0"D$ ֜2 +8 ."detached from bill"Carousel.configure :default +2 "carousel0"F#<` F 22 +10 ."detached from bill"Abs.configure :default +2 "ambient0"J#  +10 ."detached from bill"PhaseSeparator.configure :default + "carousel1"G$ +10 ."detached from bill"Archive.configure :default +2 "syringe0"B$d PZ<2 +9 ."detached from bill"Air.configure :default + "syringe1"C#d P]z<2 +6 ."detached from bill"Lysis1.configure :default +2 "syringe1"C$d P[<2 +6 ."detached from bill"Lysis2.configure :default +2 "syringe0"B#d P\\k<2 +10 ."detached from bill"PinPort.configure :default +2 "valve1"E$ B22 +6 #"detached from bill"All elf controllers running firmware version 4.94 +3 .Gate.power :motor,:ON,limit to 5000mA +8 "valve0"Dp% +6 D& +7 "core" %GO%  +2 "carousel0"Fi% +6 "syringe0"Bf& +6 ."Plunge_home"Plunge.home.jog 0 +17 "syringe1"Cb% +6 C& +5 "syringe0"Br% +4 "valve1"Em& +3 "valve0"D) +17 "core" Đ&+aO" q + %N5OQ H + &i  %݇f + Ù&U1 _ + %٣i  + &O , + ā) +8 "valve0"D%% +7 ."PinPort_home"PinPort.home.jog 0 +3 ."Lysis1_home"Lysis1.home.jog 0 +9 ."Archive_home"Archive.home.jog 0 +5 ."Carousel_home"Carousel.home.jog 0 +4 ."Toroid_home"Toroid.home.jog 0 +4 ."Lysis2_home"Lysis2.home.jog 0 +5 ."Air_home"Air.home.jog 0 +4 "valve1"E* +3 "syringe1"Ct* +8 "syringe0"BN* +5 "carousel0"F) +3 "valve0"D[* +4 "syringe1"Co) +4 "syringe0"B`) +23 "core" ĥ%HFO  + Œ* + * * + * + ƚ) + ) ) +3 "syringe0"B+% +16 "syringe1"C>& +2 "valve1"Ez& +3 "syringe0"B;& +4 "syringe1"C\ % +2 ."Plunge_home"Plunge.in :homing +5 "valve0"D& +2 D> (2 +22 ."Plunge_home"Plunge.jog 1490 +5 "valve0"D^) +6 "core" «%uٯh + þ&Rg + &#O  + »&s Ê%݂$ + Ė&*O +8 ."Lysis2_home"Lysis2.in :homing +7 ."Lysis1_home"Lysis1.in :homing +4 ."Archive_home"Archive.in :homing +5 ."PinPort_home"PinPort.in :homing +5 ."Air_home"Air.in :homing +4 ."Toroid_home"Toroid.in :homing +4 "syringe0"B> (2 +3 "syringe1"C? (2 +4 "syringe0"B? -2 +3 "valve1"E?2 +5 "syringe1"C> -2 +2 "valve0"D?2 +2 ."Lysis2_home"Lysis2.jog 1616 +8 ."Lysis1_home"Lysis1.jog 1602 +8 "syringe1"C'*B +11 "syringe0"B0)P +4 ."PinPort_home"PinPort.jog 1243 +3 ."Air_home"Air.jog 1432 +8 ."Archive_home"Archive.jog 1618 +11 ."Toroid_home"Toroid.jog 1350 +8 "valve1"EP* +4 "syringe1"C&) +6 "syringe0"BG*R +4 "valve0"DJ*F +17 "core" ) +2 ."Plunge_home"Plunge.setRawPosition! 50 + "valve0"D72 +7 ."Plunge_home"Plunge.seek :home +3 "valve0"D' +25 "core" ĕ' +2 ."Plunge_home"Plunge.in :default +2 "valve0"D> P ޤ-2 +25 "core" ç* +2 ."Lysis1_home"Lysis1.setRawPosition! 50 + "syringe1"C82 +8 ."Lysis1_home"Lysis1.seek :home +4 "syringe1"C,( +6 "core" °) +2 ."Lysis2_home"Lysis2.setRawPosition! 50 + "syringe0"B72 +7 ."Lysis2_home"Lysis2.seek :home +3 "syringe0"Ba' +8 "core" æ) + ì( + * +3 ."Air_home"Air.setRawPosition! 0 +4 ."Archive_home"Archive.setRawPosition! 50 +5 ."Lysis1_home"Lysis1.in :default +5 "syringe1"C7 +5 "syringe0"B82 + "syringe1"C?d P[<2 +7 ."Air_home"Air.seek :home +67 ."Archive_home"Archive.seek :home +10 "syringe1"C|' +2 "syringe0"B( +11 "core" ' +6 * + ' +5 ."Lysis2_home"Lysis2.in :default +2 ."PinPort_home"PinPort.setRawPosition! 3150 +4 ."Air_home"Air.in :default +6 "syringe0"B>d P\\k<2 +2 "valve1"E8 N +5 "syringe1"C>d P]z<2 +8 ."PinPort_home"PinPort.seek :home +3 "valve1"Ej( +9 "core" ƒ( +2 ."Archive_home"Archive.in :default +2 "syringe0"B?d PZ<2 +145 "core" * +3 ."Toroid_home"Toroid.setRawPosition! 0 +2 "valve0"D8 +7 ."Toroid_home"Toroid.seek :home +3 "valve0"D ( +4 "core" Ġ( +3 ."Toroid_home"Toroid.in :default +3 "valve0"D? ֜2 +252 "core" ( +3 ."PinPort_home"PinPort.in :default + "valve1"E? B22 +36 ."clearToroid"Toroid.seek :clear +3 "valve1"EX& +5 "valve0"Dl( +6 "core" &O +5 ."detached from bill"PinPort.in :unlocking +3 "valve1"E? B2 +7 ."detached from bill"PinPort.seek :clear +3 "valve1"E( +42 "core" Ŏ( +289 ( +3 ."detached from bill"Air.seek :clear +3 "syringe1"C4' +9 "core" ô' +2 ."detached from bill"Lysis1.seek :clear +3 "syringe1"Cv( +69 "core" ( +3 ."detached from bill"Lysis2.seek :clear +3 "syringe0"B.'j +76 "core" ®' +3 ."detached from bill"Archive.seek :clear +3 "syringe0"Bs(j +76 "core" ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +7 Fu% +4 "core" % V5OQ  +4 ."detached from bill"Carousel.home.seek 13 +3 "carousel0"F' +16 "core" Ɨ' +2 "valve0"Dw% +6 "core" %^O, !u +3 #"detached from bill"Selecting Cartridge 12 +2 "valve0"D% +8 "core" ě%^O0 ! +2 "syringe1"C1& +4 "core" ñ&FY +3 "syringe1"C/% +4 "core" ï%ݓԁ +3 "valve1"E{& +5 "core" &O/ ; +3 ."detached from bill"Carousel.seek 12 +3 "carousel0"F'\ +45 "core" Ɵ' +5 "carousel0"FH% +3 "core" %\ *1S 2 +3 ."detached from bill"Carousel.reconfigure :align +2 "carousel0"F#` F 22 +7 "syringe1"C@& +4 "core" &IV +3 ."detached from bill"Lysis1.in :fast +2 "syringe1"C?d P[<2 +6 ."detached from bill"Lysis1.seek :locked +2 "syringe1"CC( +250 "core" ( +3 "valve0"D:% +13 "core" ĺ%^wOU ! +3 ."Plunge"Plunge.seek :locked +3 "valve0"Dx'Z +7 "syringe1"C?% +6 "core" ÿ%݆X +3 ."Air"Air.in :fast +2 "syringe1"C> P]z<2 +7 ."Air"Air.seek :locked +3 "syringe1"CL'6 +80 "core" ' +24 ' +2 ."detached from bill"Carousel.reconfigure :locked +2 "carousel0"F#` F 2\ 2 +7 FA% +3 "core" %\ W1 3P  +6 #"detached from bill"Powering cartridge bus +264 "cartridge_broadcast" ( +3 ."detached from bill"Gate.setIR 1750,5000,0,6 +14 .Gate.setIR 0,5000,0,6 +2 "cartridge_broadcast"~ +7 ."detached from bill"PuckHeater.configure :default +2 "Cartridge" \ :D H  +7 ."detached from bill"Gate.power :heat,:ON,limit to 5000mA +5 "Cartridge" Sy +3 "cartridge" y4646 +4 "Cartridge" Uy  +6 "cartridge" y :L+0J59912 N+0ŊCxޜ +3 #"detached from bill"Mounted lysis Cartridge board#J59912 +6 #Using default cartridge heat loss curve for HeatingFunctionInCarousel +5 "valve0"DM% +6 "core" %ZFO! q +3 "syringe1"CO& +5 "core" &M +2 "syringe1"C% +4 "core" Ç%6L k +3 "carousel0"F% +5 "core" ƈ%\ 1 ]PY \ +3 "carousel0"F(% +6 "core" ƨ%\ 1 dP8 \ +117 ."PROCESSING"SPR.command "read_sequences" +7 .SPR.command "stats_on" +82 "carousel0"F3% +4 "core" Ƴ%\ 1 Pj \ +56 ."PROCESSING"SPR.command "start 0 1" +21 ."SPRlogger"SPR.start /var/log/esp/spr26aug0205h.spr.bz2 +202 #"PROCESSING"Waiting for baseline SPR stabilization +2 .Handoff.select :waste +3 "valve0"D +52 #"PROCESSING"Waiting up to 3:00 for Syringe to stabilize +/-0.20psi +2 "syringe1"C5% +6 "core" õ%6K n +45 "syringe1"C% +4 "core" Ï%6G u +45 "syringe1"C % +4 "core" É%6I q +47 "syringe1"C-% +4 "core" í%6H q +46 "syringe1"Cy% +3 "core" %6L h +47 "syringe1"C% +4 "core" Ñ%6 p +46 "syringe1"C% +3 "core" %6J x +47 "syringe1"C % +3 "core" Ì%6M u +47 "syringe1"Cd% +3 "core" %6D r +47 "syringe1"C\\% +3 "core" %6G p +33 "Can"lX,lyu?.|x6z +14 "syringe1"CR% +4 "core" %6L p +46 "syringe1"CW% +4 "core" %6I q +46 "syringe1"C_% +4 "core" %6K o +48 "syringe1"C*% +6 "core" ê%6D s +5 "Can"lX,lyum|6{D + #"PROCESSING"Syringe tare is 51% of full scale at 24C (drifted -0%) +5 .PinPort.in :locking +2 "valve1"E? B22 +6 ."PROCESSING"PinPort.seek :locked +3 "valve1"E=( N +81 "core" Ž( +5 ."posArchive"Archive.in :fast +4 "syringe0"B?d PZ<2 +7 ."posLyS2"Lysis2.in :fast +8 ."PROCESSING"Lysis1.seek 950uL +2 "syringe0"B>d P\\k<2 +5 ."posArchive"Archive.seek :empty +6 "syringe1"Cg( +2 ."posLyS2"Lysis2.seek 950uL +10 "syringe0"B2( +5 B6' +346 "core" ( +156 ¶' +327 ²( +2 ."PROCESSING"delayUntil "05:16:59.57" +10808 "Can"lX,kyu9ID6 +11966 jX1,jyu=Mw6 +11962 lX,ayu76 +11964 lX,ayu>n6 +11553 ."PROCESSING"Lysis2.in :DAmix +2 "syringe0"B>d2 P\\k<2 +6 B% +4 "core" †%z +3 ."PROCESSING"Lysis1.in :DAmix + "syringe1"C?d2 P[<2 +10 C& +3 "core" Ý&z +59 ."Lysis2mixer"Lysis2.seek 700uL +4 ."PROCESSING"Lysis1.seek 700uL +6 "syringe0"B"'| +5 "syringe1"CB(| +308 "Can"iW,ays\\@7 +3178 "core" ¢' +4 ( +2 ."PROCESSING"delay 5 seconds +503 .Handoff.select SPR +3 "valve0"D@ +49 ."PROCESSING"SPR.command "start 2 14" +114 #SPR stabilized + "syringe1"C]& +6 "core" &|eb +3 "syringe0"BT% +6 "core" %|e +2 ."PROCESSING"delay 20 seconds +2003 .SPR.command "start 2 0" +314 #Waiting up to 11:00 for SPR to enter state 1 +5776 "Can"mW,jyu17. +6033 ."PROCESSING"SPR.command "hold_on" +192 #SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BQ% +6 "core" %|e +3 "syringe1"C<& +6 "core" ü&|ee +55 ."Lysis2mixer"Lysis2.seek 400uL +5 "syringe0"Bc' +11 ."PROCESSING"Lysis1.seek 400uL +9 "syringe1"Ce( +4171 "core" ' +19 ( +3 ."PROCESSING"delay 15 seconds +1446 "Can"nWP,oyt.?7L +56 ."PROCESSING"SPR.command "hold_off" +313 #Waiting up to 1:00:00 for SPR to enter state 7 +11594 "Can"kW(,qyu/7j +11961 kW,pyu0*7 +11957 lW,oyu07 +11960 mX>,fyu,'7 +11964 kXc,cyu/7 +11966 kX,Zyu.P8 +11963 kX,Wyu-8 +11965 kX,Lyu/K8< +2133 #"SPRlogger"SPRsummary:1.26RIU,15.1RIU,2.87RIU,16RIU after 1925s +9830 "Can"mX,Eyu$/j8Z +11962 iX,@yu #8x +11960 iX,Cyu$8 +11964 lX,Byu$Zl8 +11963 jXW,Iyu$˦8 +11961 jX8,Myu.v8 +11965 lW,Pyu/9 +4086 "syringe1"Ch& +3 "core" &Ky ? +5 "syringe0"BV% +5 "core" %L#x  +2 ."PROCESSING"delay 20 seconds +2002 .SPR.command "start 2 0" +309 #Waiting up to 11:00 for SPR to enter state 1 +5554 "Can"kXx,Iyu(r9, +6212 ."PROCESSING"SPR.command "hold_on" +138 #SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 +3 "syringe0"BD% +7 "core" %L%L  +4 "syringe1"C~& +5 "core" &KH A +59 ."Lysis2mixer"Lysis2.seek 100uL +6 ."PROCESSING"Lysis1.seek 100uL +6 "syringe0"BE'ނ +4 "syringe1"C(ނ +4181 "core" ' +4 Ä( +2 ."PROCESSING"delay 15 seconds +1334 "Can"jY,Myt.9J +168 ."PROCESSING"SPR.command "hold_off" +335 #Waiting up to 1:00:00 for SPR to enter state 7 +11461 "Can"kZ,Myu/@9h +11963 jZ,Pyu39 +11961 kZ,Nyu0:9 +11967 kZ,Lyu/9 +11967 kY,Pyu/39 +11969 lYi,Syu/9 +11972 mX,Zyu._: +11971 kX,`yu(K:: +11970 kX,_yu $ :X +11974 kXJ,^yu\ #4:v +11970 kW,byu #j: +11970 jW,fyu-\ : +11971 jW,nyu$1: +11968 lX%,syu.!: +11966 jY$,vyu/; +4270 #"PROCESSING"SPR analysis completed +66 #"SPRlogger"Ended log spr26aug0205h.spr.bz2 +3 ."PROCESSING"SPR.command "stats_off" +93 "carousel0"F)% +3 "core" Ʃ%\ 1 P \ +6 "sampler0"H7% +5 "core" ȷ%I +4 ."closeHandoff"Handoff.select :close +4 "valve0"D +51 ."detached from bill"Gate.power :heat,:OFF +3 .Gate.power :cartridge,:OFF +4 "valve1"E& +5 "core" Ŝ& N<OD +3 ."detached from bill"PinPort.in :unlocking +2 "valve1"E? B2 +6 ."detached from bill"PinPort.seek :clear +3 "valve1"E( +80 "core" Ŕ( +3 ."detached from bill"Carousel.in :default + "carousel0"F><` F 22 +8 F% +3 "core" ƅ%\ 1\ uPS \ +4 ."detached from bill"Carousel.home.seek 12 +4 "carousel0"Fk'\ +15 "core" ' +2 "carousel0"FK% +4 "core" %\ 21\ T& 2 +4 "syringe1"C#& +4 "core" ã&ނ2r +3 ."detached from bill"Plunge.seek :locked +3 "valve0"D 'Z +8 "core" č' +2 "syringe0"B& +4 "core" ž&)d +5 ."Archive"Archive.in :unlocking +3 "syringe0"B? PZ-2 +5 "syringe1"C!% +7 ."Archive"Archive.seek :clear +2 "syringe0"B (j +7 "core" á%6ɹn +4 ."Air"Air.in :unlocking +4 "syringe1"CF& +3 C> P]z-2 +9 ."Air"Air.seek :clear +2 "syringe1"C' +7 "core" &ނ2 +4 ."Lysis1"Lysis1.in :unlocking +3 "syringe1"C? P[(2 +5 "syringe0"BI% +64 ."Lysis1"Lysis1.seek :clear +3 "syringe1"C9( +8 "core" %ނ3z +6 ."Lysis2"Lysis2.in :unlocking +2 "syringe0"B> P\\k(2 +5 "core" Â' +5 ."Lysis2"Lysis2.seek :clear +3 "syringe0"B}'j +319 "core" ‹( +98 ù( +26 ' +4 "sampler0"Hp% +4 "core" %艢 +11 ."detached from bill"Gate.power :motor,:OFF +4 .Gate.power :analytic1,:OFF +3 .Gate.power :core,:OFF +4 .Gate.power :main,:OFF +3 #Safely stopped and ready to power off @1785666345.15EDT @EDT1785666345.33 !o:Cartridge::None: mesg"*Not enough standard cartridges remain:bt["7/home/esp/esp2/bin/fireAll:291:in `checkInventory'"-/home/esp/esp2/bin/fireAll:289:in `each'"7/home/esp/esp2/bin/fireAll:289:in `checkInventory'"#/home/esp/esp2/bin/fireAll:318"-/home/esp/esp2/bin/fireAll:317:in `loop'"#/home/esp/esp2/bin/fireAll:317";/home/esp/esp2/lib/framework.rb:199:in `instance_exec'"7/home/esp/esp2/lib/framework.rb:199:in `runScript'"4/home/esp/esp2/utils/init3g.rb:277:in `fireAll'"!bill:45:in `topLevelBinding'"./home/esp/esp2/lib/base.rb:296:in `start'"7/home/esp/esp2/lib/schedule.rb:497:in `initialize'"0/home/esp/esp2/lib/thread.rb:736:in `start'"7/home/esp/esp2/lib/schedule.rb:490:in `initialize'"'/home/esp/esp2/lib/schedule.rb:488 +6621 "Can"hY,qyu;* +11969 mZ,byu*;H +11969 oY,Zyu;f +11970 kY,Tyu@; +11965 jY,Nyu; +11965 nYG,OyuV; +11966 mY,Kyu; +11966 mX,Lyuk; +11970 mX,Nyu< +11967 kX,Qyu<8 +11970 mXN,Kyu