@19:40:07.80EDT28-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @19:40:09.58 >BREAK< ESP3 Gateway v1.44 for core bus @19:40:09.61 >BREAK< ESP3 Gateway v1.44 for cartridge bus @19:40:13.52 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @19:40:14.26 Sea pressure (Abs) tare is 6% of full scale @19:40:16.65 Polling Can every 2:00 @19:40:16.75 Waiting for espclients... @19:40:17.71 Session Begun @19:40:29.12 -> slots {} @19:43:36.63 -> slot[60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:1525, lysed2:1525}] @19:43:36.68 SyntaxError in tina -- compile error tina:2: syntax error, unexpected tASSOC, expecting $end ...0,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {... ^ @19:44:11.05 -> slot [60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:1525, lysed2:1525}] {[60,54,48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 1525, lysed2: 1525}} @19:45:40.97 -> Cartridge.aspects {standard: {extraDilutions: [1,1], lysed: 950, lysed2: 950}} @19:46:23.38 -> Cart.aspectsOf :standard, extraDilutions:[1], lysed:1525, lysed2:1525 {extraDilutions: [1], lysed: 1525, lysed2: 1525} @19:46:34.43 -> Cartridge.aspects {standard: {extraDilutions: [1], lysed: 1525, lysed2: 1525}} @19:51:45.48 -> start {fireAll "standard"} # @19:51:46.40 Cartridge group: standard @19:51:46.50 Gate.power :sampler,:OFF,limit to 3500mA @19:51:46.52 Gate.power :skimmer,:OFF,limit to 3500mA @19:51:46.57 Gate.power :main,:ON,limit to 5000mA @19:51:46.61 Gate.power :analytic1,:ON,limit to 4000mA @19:51:46.81 Powering core bus @19:51:49.05 SPR Module 1, FW Rev. B, FPGA Rev. C @19:51:49.58 Plunge.configure :default @19:51:49.70 SamplePump.configure :default @19:51:49.84 Toroid.configure :default @19:51:49.95 Carousel.configure :default @19:51:50.05 Abs.configure :default @19:51:50.15 PhaseSeparator.configure :default @19:51:50.27 Archive.configure :default @19:51:50.38 Air.configure :default @19:51:50.46 Lysis1.configure :default @19:51:50.54 Lysis2.configure :default @19:51:50.65 PinPort.configure :default @19:51:50.74 All elf controllers running firmware version 4.94 @19:51:50.78 Gate.power :motor,:ON,limit to 5000mA @19:51:51.09 Plunge.home.jog 0 @19:51:51.86 Lysis2.home.jog 0 @19:51:51.91 Lysis1.home.jog 0 @19:51:51.94 Archive.home.jog 0 @19:51:51.98 Toroid.home.jog 0 @19:51:52.03 PinPort.home.jog 0 @19:51:52.09 Carousel.home.jog 0 @19:51:52.15 Air.home.jog 0 @19:51:52.90 Plunge.in :homing @19:51:53.14 Plunge.jog 1470 @19:51:53.40 PinPort.in :homing @19:51:53.48 Lysis2.in :homing @19:51:53.53 Air.in :homing @19:51:53.56 Toroid.in :homing @19:51:53.60 Lysis1.in :homing @19:51:53.68 Archive.in :homing @19:51:53.91 PinPort.jog 1206 @19:51:53.99 Lysis2.jog 1616 @19:51:54.21 Lysis1.jog 2269 @19:51:54.28 Air.jog 1243 @19:51:54.34 Archive.jog 301 @19:51:54.38 Toroid.jog 1366 @19:51:54.71 Plunge.setRawPosition! 50 @19:51:54.79 Plunge.seek :home @19:51:55.09 Plunge.in :default @19:51:55.19 Archive.setRawPosition! 50 @19:51:55.29 Archive.seek :home @19:51:55.48 Lysis2.setRawPosition! 50 @19:51:55.59 Lysis2.seek :home @19:51:55.72 Air.setRawPosition! 0 @19:51:55.82 Archive.in :default @19:51:55.96 Air.seek :home @19:51:56.15 PinPort.setRawPosition! 3150 @19:51:56.20 Lysis2.in :default @19:51:56.24 Air.in :default @19:51:56.28 Lysis1.setRawPosition! 50 @19:51:56.46 PinPort.seek :home @19:51:56.60 Lysis1.seek :home @19:51:57.36 Lysis1.in :default @19:51:59.12 Toroid.setRawPosition! 0 @19:51:59.20 Toroid.seek :home @19:51:59.33 Toroid.in :default @19:52:00.97 PinPort.in :default @19:52:01.34 Toroid.seek :clear @19:52:01.53 PinPort.in :unlocking @19:52:01.62 PinPort.seek :clear @19:52:05.03 Air.seek :clear @19:52:05.16 Lysis1.seek :clear @19:52:05.92 Lysis2.seek :clear @19:52:06.74 Archive.seek :clear @19:52:07.56 Carousel.in :default @19:52:07.71 Carousel.home.seek 6 @19:52:08.06 Selecting Cartridge 60 @19:52:08.39 Carousel.seek 60 @19:52:19.80 Carousel.reconfigure :align @19:52:19.96 Lysis1.in :fast @19:52:20.05 Lysis1.seek :locked @19:52:20.17 Slide::Warning in core -- Storage Carousel (SC) reseek at 60 + 3 counts (sensed 60 + 4 counts) @19:52:22.78 Plunge.seek :locked @19:52:22.98 Air.in :fast @19:52:23.07 Air.seek :locked @19:52:24.17 Carousel.reconfigure :locked @19:52:24.35 Powering cartridge bus @19:52:27.02 Gate.setIR 1750,5000,0,6 @19:52:27.15 Gate.setIR 0,5000,0,6 @19:52:27.25 PuckHeater.configure :default @19:52:27.34 Gate.power :heat,:ON,limit to 5000mA @19:52:27.60 Mounted lysis Cartridge board#J60008 @19:52:27.66 Using default cartridge heat loss curve for HeatingFunctionInCarousel @19:52:29.27 SPR.command "read_sequences" @19:52:29.62 SPR.command "stats_on" @19:52:29.82 SPR.command "start 0 1" @19:52:30.50 SPR.start /var/log/esp/spr26jul2819h.spr.bz2 @19:52:32.48 Waiting for baseline SPR stabilization @19:52:32.50 Handoff.select :waste @19:52:33.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:52:39.69 Syringe tare is 50% of full scale at 23C (drifted -1%) @19:52:39.74 PinPort.in :locking @19:52:39.88 PinPort.seek :locked @19:52:40.76 Archive.in :fast @19:52:40.87 Lysis2.in :fast @19:52:40.94 Lysis1.seek 1525uL @19:52:41.04 Archive.seek :empty @19:52:41.17 Lysis2.seek 1525uL @19:52:49.55 delayUntil "20:02:32.01" @19:53:02.49 -> exit @19:53:02.52 Session Ended @20:02:32.03 Lysis2.in :DAmix @20:02:32.14 Lysis1.in :DAmix @20:02:32.71 Lysis2.seek 1275uL @20:02:32.76 Lysis1.seek 1275uL @20:03:07.74 delay 5 seconds @20:03:12.77 Handoff.select SPR @20:03:13.27 SPR.command "start 2 14" @20:03:15.19 SPR stabilized @20:03:15.34 delay 20 seconds @20:03:35.36 SPR.command "start 2 0" @20:03:38.48 Waiting up to 11:00 for SPR to enter state 1 @20:05:36.11 SPR.command "hold_on" @20:05:37.53 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:05:38.18 Lysis2.seek 975uL @20:05:38.24 Lysis1.seek 975uL @20:06:20.15 delay 15 seconds @20:06:35.17 SPR.command "hold_off" @20:06:38.19 Waiting up to 1:00:00 for SPR to enter state 7 @20:22:53.74 SPRsummary:3.31RIU,243RIU,256RIU,348RIU after 1925s @20:37:10.80 delay 20 seconds @20:37:30.82 SPR.command "start 2 0" @20:37:33.89 Waiting up to 11:00 for SPR to enter state 1 @20:39:31.58 SPR.command "hold_on" @20:39:32.96 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:39:34.07 Lysis2.seek 825uL @20:39:34.11 Lysis1.seek 825uL @20:39:55.17 delay 15 seconds @20:40:10.20 SPR.command "hold_off" @20:40:12.80 Waiting up to 1:00:00 for SPR to enter state 7 @21:10:45.29 SPR analysis completed @21:10:45.88 Ended log spr26jul2819h.spr.bz2 @21:10:45.90 SPR.command "stats_off" @21:10:47.21 Handoff.select :close @21:10:47.73 Gate.power :heat,:OFF @21:10:47.74 Gate.power :cartridge,:OFF @21:10:47.86 PinPort.in :unlocking @21:10:47.91 PinPort.seek :clear @21:10:48.73 Carousel.in :default @21:10:48.85 Carousel.home.seek 60 @21:10:49.17 Plunge.seek :locked @21:10:49.35 Archive.in :unlocking @21:10:49.48 Archive.seek :clear @21:10:49.61 Air.in :unlocking @21:10:49.72 Air.seek :clear @21:10:49.90 Lysis1.in :unlocking @21:10:49.99 Lysis1.seek :clear @21:10:50.05 Lysis2.in :unlocking @21:10:50.17 Lysis2.seek :clear @21:10:54.33 Gate.power :motor,:OFF @21:10:54.35 Gate.power :analytic1,:OFF @21:10:54.36 Gate.power :core,:OFF @21:10:54.38 Gate.power :main,:OFF @21:10:54.39 Safely stopped and ready to power off @21:10:54.98 Gate.power :sampler,:OFF,limit to 3500mA @21:10:54.99 Gate.power :skimmer,:OFF,limit to 3500mA @21:10:58.19 Gate.power :main,:ON,limit to 5000mA @21:10:58.20 Gate.power :analytic1,:ON,limit to 4000mA @21:10:58.27 Powering core bus @21:11:00.66 SPR Module 1, FW Rev. B, FPGA Rev. C @21:11:01.59 Plunge.configure :default @21:11:01.67 SamplePump.configure :default @21:11:01.75 Toroid.configure :default @21:11:01.86 Carousel.configure :default @21:11:01.94 Abs.configure :default @21:11:02.02 PhaseSeparator.configure :default @21:11:02.10 Archive.configure :default @21:11:02.18 Air.configure :default @21:11:02.23 Lysis1.configure :default @21:11:02.28 Lysis2.configure :default @21:11:02.37 PinPort.configure :default @21:11:02.42 All elf controllers running firmware version 4.94 @21:11:02.44 Gate.power :motor,:ON,limit to 5000mA @21:11:02.89 Plunge.home.jog 0 @21:11:03.43 Lysis2.home.jog 0 @21:11:03.48 Air.home.jog 0 @21:11:03.51 Archive.home.jog 0 @21:11:03.55 Toroid.home.jog 0 @21:11:03.58 PinPort.home.jog 0 @21:11:03.62 Lysis1.home.jog 0 @21:11:03.65 Carousel.home.jog 0 @21:11:04.56 Plunge.in :homing @21:11:04.85 Plunge.jog 1480 @21:11:04.92 PinPort.in :homing @21:11:04.96 Lysis1.in :homing @21:11:04.97 Archive.in :homing @21:11:05.00 Air.in :homing @21:11:05.02 Toroid.in :homing @21:11:05.04 Lysis2.in :homing @21:11:05.26 PinPort.jog 1227 @21:11:05.48 Lysis2.jog 1618 @21:11:05.53 Lysis1.jog 1603 @21:11:05.61 Archive.jog 1626 @21:11:05.62 Toroid.jog 1359 @21:11:05.65 Air.jog 1429 @21:11:06.37 Plunge.setRawPosition! 50 @21:11:06.42 Plunge.seek :home @21:11:06.70 Plunge.in :default @21:11:06.99 Lysis1.setRawPosition! 50 @21:11:07.05 Lysis1.seek :home @21:11:07.12 Lysis2.setRawPosition! 50 @21:11:07.23 Lysis2.seek :home @21:11:07.34 PinPort.setRawPosition! 3150 @21:11:07.37 Air.setRawPosition! 0 @21:11:07.40 Archive.setRawPosition! 50 @21:11:07.55 PinPort.seek :home @21:11:07.59 Air.seek :home @21:11:07.62 Archive.seek :home @21:11:07.91 Lysis1.in :default @21:11:07.94 Air.in :default @21:11:07.96 Lysis2.in :default @21:11:08.16 Archive.in :default @21:11:10.41 Toroid.setRawPosition! 0 @21:11:10.46 Toroid.seek :home @21:11:10.54 Toroid.in :default @21:11:12.09 PinPort.in :default @21:11:12.47 Toroid.seek :clear @21:11:13.01 PinPort.in :unlocking @21:11:13.07 PinPort.seek :clear @21:11:16.07 Air.seek :clear @21:11:16.25 Lysis1.seek :clear @21:11:16.97 Lysis2.seek :clear @21:11:17.76 Archive.seek :clear @21:11:18.57 Carousel.in :default @21:11:18.69 Carousel.home.seek 60 @21:11:18.96 Selecting Cartridge 54 @21:11:19.26 Carousel.seek 54 @21:11:20.84 Carousel.reconfigure :align @21:11:20.96 Lysis1.in :fast @21:11:21.01 Lysis1.seek :locked @21:11:21.15 Slide::Warning in core -- Storage Carousel (SC) reseek at 54 - 4 counts @21:11:23.64 Plunge.seek :locked @21:11:23.84 Air.in :fast @21:11:23.90 Air.seek :locked @21:11:24.97 Carousel.reconfigure :locked @21:11:25.14 Powering cartridge bus @21:11:27.84 Gate.setIR 1750,5000,0,6 @21:11:27.97 Gate.setIR 0,5000,0,6 @21:11:28.04 PuckHeater.configure :default @21:11:28.10 Gate.power :heat,:ON,limit to 5000mA @21:11:28.27 Mounted lysis Cartridge board#J59921 @21:11:28.30 Using default cartridge heat loss curve for HeatingFunctionInCarousel @21:11:29.87 SPR.command "read_sequences" @21:11:29.93 SPR.command "stats_on" @21:11:30.10 SPR.command "start 0 1" @21:11:30.79 SPR.start /var/log/esp/spr26jul2821h.spr.bz2 @21:11:32.70 Waiting for baseline SPR stabilization @21:11:32.72 Handoff.select :waste @21:11:33.22 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:11:40.06 Syringe tare is 50% of full scale at 23C (drifted -1%) @21:11:40.09 PinPort.in :locking @21:11:40.14 PinPort.seek :locked @21:11:40.98 Archive.in :fast @21:11:41.08 Lysis2.in :fast @21:11:41.12 Lysis1.seek 1525uL @21:11:41.18 Archive.seek :empty @21:11:41.26 Lysis2.seek 1525uL @21:11:49.69 delayUntil "21:21:32.27" @21:21:32.29 Lysis2.in :DAmix @21:21:32.40 Lysis1.in :DAmix @21:21:32.95 Lysis2.seek 1275uL @21:21:33.01 Lysis1.seek 1275uL @21:22:07.99 delay 5 seconds @21:22:13.01 Handoff.select SPR @21:22:13.52 SPR.command "start 2 14" @21:22:14.91 SPR stabilized @21:22:15.08 delay 20 seconds @21:22:35.10 SPR.command "start 2 0" @21:22:37.77 Waiting up to 11:00 for SPR to enter state 1 @21:24:35.42 SPR.command "hold_on" @21:24:36.81 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:24:37.44 Lysis2.seek 975uL @21:24:37.49 Lysis1.seek 975uL @21:25:19.43 delay 15 seconds @21:25:34.45 SPR.command "hold_off" @21:25:37.46 Waiting up to 1:00:00 for SPR to enter state 7 @21:41:53.03 SPRsummary:4.26RIU,230RIU,245RIU,344RIU after 1924s @21:56:10.08 delay 20 seconds @21:56:30.11 SPR.command "start 2 0" @21:56:33.19 Waiting up to 11:00 for SPR to enter state 1 @21:58:30.87 SPR.command "hold_on" @21:58:32.24 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:58:32.94 Lysis2.seek 825uL @21:58:32.99 Lysis1.seek 825uL @21:58:54.05 delay 15 seconds @21:59:09.07 SPR.command "hold_off" @21:59:12.09 Waiting up to 1:00:00 for SPR to enter state 7 @21:59:24.12 Session Begun @21:59:29.54 -> cd /varl/log/esp @21:59:29.58 SyntaxError in bill -- compile error bill:1: unknown regexp options - lg @21:59:36.58 -> ls -ls @21:59:36.62 NoMethodError in bill -- undefined method `-@' for :ls:Symbol @21:59:47.46 -> slots {[48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 1525, lysed2: 1525}, 54 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: spr26jul2821h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2819h.spr.bz2}} @22:00:14.50 -> exit @22:00:14.53 Session Ended @22:21:17.20 Session Begun @22:21:20.28 -> slot {[48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 1525, lysed2: 1525}, 54 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: spr26jul2821h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2819h.spr.bz2}} @22:21:32.55 -> showlog -100 @22:29:44.56 SPR analysis completed @22:29:45.14 Ended log spr26jul2821h.spr.bz2 @22:29:45.16 SPR.command "stats_off" @22:29:46.47 Handoff.select :close @22:29:47.02 Gate.power :heat,:OFF @22:29:47.04 Gate.power :cartridge,:OFF @22:29:47.17 PinPort.in :unlocking @22:29:47.25 PinPort.seek :clear @22:29:48.11 Carousel.in :default @22:29:48.26 Carousel.home.seek 54 @22:29:48.62 Plunge.seek :locked @22:29:48.83 Archive.in :unlocking @22:29:48.98 Archive.seek :clear @22:29:49.11 Air.in :unlocking @22:29:49.25 Air.seek :clear @22:29:49.39 Lysis1.in :unlocking @22:29:49.54 Lysis1.seek :clear @22:29:49.68 Lysis2.in :unlocking @22:29:49.79 Lysis2.seek :clear @22:29:53.81 Gate.power :motor,:OFF @22:29:53.84 Gate.power :analytic1,:OFF @22:29:53.88 Gate.power :core,:OFF @22:29:53.91 Gate.power :main,:OFF @22:29:53.96 Safely stopped and ready to power off @22:29:54.61 Gate.power :sampler,:OFF,limit to 3500mA @22:29:54.64 Gate.power :skimmer,:OFF,limit to 3500mA @22:29:57.73 Gate.power :main,:ON,limit to 5000mA @22:29:57.75 Gate.power :analytic1,:ON,limit to 4000mA @22:29:57.94 Powering core bus @22:30:00.26 SPR Module 1, FW Rev. B, FPGA Rev. C @22:30:00.73 Plunge.configure :default @22:30:00.84 SamplePump.configure :default @22:30:00.96 Toroid.configure :default @22:30:01.07 Carousel.configure :default @22:30:01.18 Abs.configure :default @22:30:01.29 PhaseSeparator.configure :default @22:30:01.40 Archive.configure :default @22:30:01.51 Air.configure :default @22:30:01.59 Lysis1.configure :default @22:30:01.67 Lysis2.configure :default @22:30:01.81 PinPort.configure :default @22:30:01.89 All elf controllers running firmware version 4.94 @22:30:01.93 Gate.power :motor,:ON,limit to 5000mA @22:30:02.18 Plunge.home.jog 0 @22:30:02.97 Lysis2.home.jog 0 @22:30:03.03 Air.home.jog 0 @22:30:03.07 Archive.home.jog 0 @22:30:03.11 Toroid.home.jog 0 @22:30:03.18 PinPort.home.jog 0 @22:30:03.23 Lysis1.home.jog 0 @22:30:03.27 Carousel.home.jog 0 @22:30:04.19 Plunge.in :homing @22:30:04.51 Plunge.jog 1482 @22:30:04.73 Lysis2.in :homing @22:30:04.81 Lysis1.in :homing @22:30:04.86 Archive.in :homing @22:30:04.90 PinPort.in :homing @22:30:04.95 Air.in :homing @22:30:04.98 Toroid.in :homing @22:30:05.31 Lysis2.jog 1621 @22:30:05.36 Lysis1.jog 1602 @22:30:05.42 Archive.jog 302 @22:30:05.47 PinPort.jog 1248 @22:30:05.52 Air.jog 1431 @22:30:05.57 Toroid.jog 1367 @22:30:06.08 Plunge.setRawPosition! 50 @22:30:06.17 Plunge.seek :home @22:30:06.30 Archive.setRawPosition! 50 @22:30:06.39 Archive.seek :home @22:30:06.54 Plunge.in :default @22:30:06.70 Archive.in :default @22:30:06.91 Lysis2.setRawPosition! 50 @22:30:07.00 Lysis2.seek :home @22:30:07.14 Lysis1.setRawPosition! 50 @22:30:07.22 Lysis1.seek :home @22:30:07.39 Air.setRawPosition! 0 @22:30:07.41 Lysis2.in :default @22:30:07.57 Air.seek :home @22:30:07.73 PinPort.setRawPosition! 3150 @22:30:07.81 Lysis1.in :default @22:30:07.87 Air.in :default @22:30:07.98 PinPort.seek :home @22:30:10.49 Toroid.setRawPosition! 0 @22:30:10.57 Toroid.seek :home @22:30:11.14 Toroid.in :default @22:30:12.47 PinPort.in :default @22:30:12.84 Toroid.seek :clear @22:30:13.05 PinPort.in :unlocking @22:30:13.13 PinPort.seek :clear @22:30:16.54 Air.seek :clear @22:30:16.68 Lysis1.seek :clear @22:30:17.42 Lysis2.seek :clear @22:30:18.24 Archive.seek :clear @22:30:19.05 Carousel.in :default @22:30:19.22 Carousel.home.seek 54 @22:30:19.53 Selecting Cartridge 48 @22:30:19.91 Carousel.seek 48 @22:30:21.52 Carousel.reconfigure :align @22:30:21.67 Lysis1.in :fast @22:30:21.79 Lysis1.seek :locked @22:30:24.45 Plunge.seek :locked @22:30:24.65 Air.in :fast @22:30:24.73 Air.seek :locked @22:30:25.82 Carousel.reconfigure :locked @22:30:26.02 Powering cartridge bus @22:30:28.68 Gate.setIR 1750,5000,0,6 @22:30:28.81 Gate.setIR 0,5000,0,6 @22:30:28.91 PuckHeater.configure :default @22:30:29.00 Gate.power :heat,:ON,limit to 5000mA @22:30:29.22 Mounted lysis Cartridge board#J59994 @22:30:29.28 Using default cartridge heat loss curve for HeatingFunctionInCarousel @22:30:30.90 SPR.command "read_sequences" @22:30:31.36 SPR.command "stats_on" @22:30:31.54 SPR.command "start 0 1" @22:30:32.25 SPR.start /var/log/esp/spr26jul2822h.spr.bz2 @22:30:34.21 Waiting for baseline SPR stabilization @22:30:34.23 Handoff.select :waste @22:30:34.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:30:41.45 Syringe tare is 50% of full scale at 23C (drifted -1%) @22:30:41.54 PinPort.in :locking @22:30:41.64 PinPort.seek :locked @22:30:42.59 Archive.in :fast @22:30:42.71 Lysis2.in :fast @22:30:42.76 Lysis1.seek 1525uL @22:30:42.88 Archive.seek :empty @22:30:42.98 Lysis2.seek 1525uL @22:30:51.40 delayUntil "22:40:33.84" @22:38:14.14 Session Ended @22:40:33.85 Lysis2.in :DAmix @22:40:33.96 Lysis1.in :DAmix @22:40:34.53 Lysis2.seek 1275uL @22:40:34.59 Lysis1.seek 1275uL @22:41:09.56 delay 5 seconds @22:41:14.58 Handoff.select SPR @22:41:15.07 SPR.command "start 2 14" @22:41:16.43 SPR stabilized @22:41:16.59 delay 20 seconds @22:41:36.61 SPR.command "start 2 0" @22:41:39.26 Waiting up to 11:00 for SPR to enter state 1 @22:43:36.94 SPR.command "hold_on" @22:43:38.32 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:43:38.94 Lysis2.seek 975uL @22:43:39.01 Lysis1.seek 975uL @22:44:20.89 delay 15 seconds @22:44:35.91 SPR.command "hold_off" @22:44:38.97 Waiting up to 1:00:00 for SPR to enter state 7 @23:00:54.54 SPRsummary:4.75RIU,260RIU,270RIU,382RIU after 1924s @23:15:11.58 delay 20 seconds @23:15:31.61 SPR.command "start 2 0" @23:15:35.22 Waiting up to 11:00 for SPR to enter state 1 @23:17:33.31 SPR.command "hold_on" @23:17:34.70 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:17:35.79 Lysis2.seek 825uL @23:17:35.90 Lysis1.seek 825uL @23:17:56.93 delay 15 seconds @23:18:11.97 SPR.command "hold_off" @23:18:14.53 Waiting up to 1:00:00 for SPR to enter state 7 @23:48:47.03 SPR analysis completed @23:48:47.56 Ended log spr26jul2822h.spr.bz2 @23:48:47.58 SPR.command "stats_off" @23:48:48.99 Handoff.select :close @23:48:49.51 Gate.power :heat,:OFF @23:48:49.53 Gate.power :cartridge,:OFF @23:48:49.61 PinPort.in :unlocking @23:48:49.66 PinPort.seek :clear @23:48:50.48 Carousel.in :default @23:48:50.60 Carousel.home.seek 48 @23:48:50.94 Plunge.seek :locked @23:48:51.11 Archive.in :unlocking @23:48:51.24 Archive.seek :clear @23:48:51.36 Air.in :unlocking @23:48:51.49 Air.seek :clear @23:48:51.55 Lysis1.in :unlocking @23:48:51.70 Lysis1.seek :clear @23:48:51.88 Lysis2.in :unlocking @23:48:51.94 Lysis2.seek :clear @23:48:56.10 Gate.power :motor,:OFF @23:48:56.12 Gate.power :analytic1,:OFF @23:48:56.13 Gate.power :core,:OFF @23:48:56.15 Gate.power :main,:OFF @23:48:56.16 Safely stopped and ready to power off @23:48:56.73 Gate.power :sampler,:OFF,limit to 3500mA @23:48:56.75 Gate.power :skimmer,:OFF,limit to 3500mA @23:49:00.49 Gate.power :main,:ON,limit to 5000mA @23:49:00.52 Gate.power :analytic1,:ON,limit to 4000mA @23:49:00.59 Powering core bus @23:49:02.95 SPR Module 1, FW Rev. B, FPGA Rev. C @23:49:03.37 Plunge.configure :default @23:49:03.45 SamplePump.configure :default @23:49:03.53 Toroid.configure :default @23:49:03.61 Carousel.configure :default @23:49:03.69 Abs.configure :default @23:49:03.81 PhaseSeparator.configure :default @23:49:03.89 Archive.configure :default @23:49:03.97 Air.configure :default @23:49:04.03 Lysis1.configure :default @23:49:04.08 Lysis2.configure :default @23:49:04.16 PinPort.configure :default @23:49:04.22 All elf controllers running firmware version 4.94 @23:49:04.24 Gate.power :motor,:ON,limit to 5000mA @23:49:04.50 Plunge.home.jog 0 @23:49:05.22 PinPort.home.jog 0 @23:49:05.25 Lysis1.home.jog 0 @23:49:05.30 Air.home.jog 0 @23:49:05.34 Carousel.home.jog 0 @23:49:05.37 Toroid.home.jog 0 @23:49:05.40 Lysis2.home.jog 0 @23:49:05.43 Archive.home.jog 0 @23:49:06.33 Plunge.in :homing @23:49:06.61 Plunge.jog 1478 @23:49:06.68 Lysis2.in :homing @23:49:06.72 Air.in :homing @23:49:06.74 Toroid.in :homing @23:49:06.76 Lysis1.in :homing @23:49:06.78 PinPort.in :homing @23:49:06.80 Archive.in :homing @23:49:07.12 Lysis2.jog 1621 @23:49:07.15 Air.jog 1433 @23:49:07.19 Toroid.jog 1380 @23:49:07.22 Lysis1.jog 1604 @23:49:07.26 PinPort.jog 1240 @23:49:07.33 Archive.jog 1626 @23:49:08.10 Plunge.setRawPosition! 50 @23:49:08.15 Plunge.seek :home @23:49:08.43 Plunge.in :default @23:49:08.56 Lysis2.setRawPosition! 50 @23:49:08.62 Lysis2.seek :home @23:49:08.71 Air.setRawPosition! 0 @23:49:08.77 Air.seek :home @23:49:08.93 Lysis2.in :default @23:49:08.95 Lysis1.setRawPosition! 50 @23:49:08.96 Archive.setRawPosition! 50 @23:49:08.99 Air.in :default @23:49:09.19 Lysis1.seek :home @23:49:09.24 Archive.seek :home @23:49:10.00 Lysis1.in :default @23:49:10.03 PinPort.setRawPosition! 3150 @23:49:10.13 PinPort.seek :home @23:49:10.24 Archive.in :default @23:49:11.94 Toroid.setRawPosition! 0 @23:49:11.99 Toroid.seek :home @23:49:12.08 Toroid.in :default @23:49:14.51 PinPort.in :default @23:49:14.85 Toroid.seek :clear @23:49:15.00 PinPort.in :unlocking @23:49:15.06 PinPort.seek :clear @23:49:18.51 Air.seek :clear @23:49:18.60 Lysis1.seek :clear @23:49:19.33 Lysis2.seek :clear @23:49:20.12 Archive.seek :clear @23:49:20.90 Carousel.in :default @23:49:21.02 Carousel.home.seek 48 @23:49:21.28 Selecting Cartridge 42 @23:49:21.58 Carousel.seek 42 @23:49:23.14 Carousel.reconfigure :align @23:49:23.27 Lysis1.in :fast @23:49:23.32 Lysis1.seek :locked @23:49:25.95 Plunge.seek :locked @23:49:26.11 Air.in :fast @23:49:26.16 Air.seek :locked @23:49:27.23 Carousel.reconfigure :locked @23:49:27.38 Powering cartridge bus @23:49:30.05 Gate.setIR 1750,5000,0,6 @23:49:30.17 Gate.setIR 0,5000,0,6 @23:49:30.24 PuckHeater.configure :default @23:49:30.30 Gate.power :heat,:ON,limit to 5000mA @23:49:30.47 Mounted lysis Cartridge board#J59976 @23:49:30.51 Using default cartridge heat loss curve for HeatingFunctionInCarousel @23:49:32.03 SPR.command "read_sequences" @23:49:32.16 SPR.command "stats_on" @23:49:32.32 SPR.command "start 0 1" @23:49:33.03 SPR.start /var/log/esp/spr26jul2823h.spr.bz2 @23:49:35.02 Waiting for baseline SPR stabilization @23:49:35.04 Handoff.select :waste @23:49:36.10 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:49:45.72 Syringe tare is 50% of full scale at 22C (drifted -1%) @23:49:45.75 PinPort.in :locking @23:49:45.84 PinPort.seek :locked @23:49:46.67 Archive.in :fast @23:49:46.75 Lysis2.in :fast @23:49:46.82 Lysis1.seek 1525uL @23:49:46.88 Archive.seek :empty @23:49:47.08 Lysis2.seek 1525uL @23:49:55.33 delayUntil "23:59:34.59" @23:59:34.60 Lysis2.in :DAmix @23:59:34.71 Lysis1.in :DAmix @23:59:35.32 Lysis2.seek 1275uL @23:59:35.36 Lysis1.seek 1275uL @00:00:10.33EDT29-Jul-26 delay 5 seconds @00:00:15.35 Handoff.select SPR @00:00:15.87 SPR.command "start 2 14" @00:00:17.24 SPR stabilized @00:00:17.38 delay 20 seconds @00:00:37.41 SPR.command "start 2 0" @00:00:40.07 Waiting up to 11:00 for SPR to enter state 1 @00:02:37.75 SPR.command "hold_on" @00:02:39.11 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:02:39.78 Lysis2.seek 975uL @00:02:39.86 Lysis1.seek 975uL @00:03:21.81 delay 15 seconds @00:03:36.84 SPR.command "hold_off" @00:03:39.81 Waiting up to 1:00:00 for SPR to enter state 7 @00:19:55.35 SPRsummary:4.37RIU,243RIU,252RIU,350RIU after 1924s @00:34:12.39 delay 20 seconds @00:34:32.41 SPR.command "start 2 0" @00:34:36.04 Waiting up to 11:00 for SPR to enter state 1 @00:36:34.12 SPR.command "hold_on" @00:36:35.50 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:36:36.24 Lysis2.seek 825uL @00:36:36.29 Lysis1.seek 825uL @00:36:57.35 delay 15 seconds @00:37:12.37 SPR.command "hold_off" @00:37:15.87 Waiting up to 1:00:00 for SPR to enter state 7 @01:07:48.78 SPR analysis completed @01:07:49.32 Ended log spr26jul2823h.spr.bz2 @01:07:49.34 SPR.command "stats_off" @01:07:50.74 Handoff.select :close @01:07:51.25 Gate.power :heat,:OFF @01:07:51.27 Gate.power :cartridge,:OFF @01:07:51.35 PinPort.in :unlocking @01:07:51.40 PinPort.seek :clear @01:07:52.22 Carousel.in :default @01:07:52.34 Carousel.home.seek 42 @01:07:52.68 Plunge.seek :locked @01:07:52.85 Archive.in :unlocking @01:07:52.99 Archive.seek :clear @01:07:53.12 Air.in :unlocking @01:07:53.23 Air.seek :clear @01:07:53.34 Lysis1.in :unlocking @01:07:53.45 Lysis1.seek :clear @01:07:53.57 Lysis2.in :unlocking @01:07:53.63 Lysis2.seek :clear @01:07:58.43 Gate.power :motor,:OFF @01:07:58.47 Gate.power :analytic1,:OFF @01:07:58.49 Gate.power :core,:OFF @01:07:58.50 Gate.power :main,:OFF @01:07:58.51 Safely stopped and ready to power off @01:07:58.72 Gate.power :sampler,:OFF,limit to 3500mA @01:07:58.74 Gate.power :skimmer,:OFF,limit to 3500mA @01:08:02.31 Gate.power :main,:ON,limit to 5000mA @01:08:02.33 Gate.power :analytic1,:ON,limit to 4000mA @01:08:02.40 Powering core bus @01:08:04.76 SPR Module 1, FW Rev. B, FPGA Rev. C @01:08:05.19 Plunge.configure :default @01:08:05.27 SamplePump.configure :default @01:08:05.35 Toroid.configure :default @01:08:05.43 Carousel.configure :default @01:08:05.51 Abs.configure :default @01:08:05.59 PhaseSeparator.configure :default @01:08:05.67 Archive.configure :default @01:08:05.76 Air.configure :default @01:08:05.84 Lysis1.configure :default @01:08:05.90 Lysis2.configure :default @01:08:05.98 PinPort.configure :default @01:08:06.03 All elf controllers running firmware version 4.94 @01:08:06.06 Gate.power :motor,:ON,limit to 5000mA @01:08:06.21 Plunge.home.jog 0 @01:08:06.96 PinPort.home.jog 0 @01:08:07.02 Lysis2.home.jog 0 @01:08:07.05 Lysis1.home.jog 0 @01:08:07.08 Archive.home.jog 0 @01:08:07.12 Toroid.home.jog 0 @01:08:07.17 Air.home.jog 0 @01:08:07.22 Carousel.home.jog 0 @01:08:08.20 Plunge.in :homing @01:08:08.39 Plunge.jog 1489 @01:08:08.55 Lysis1.in :homing @01:08:08.66 Lysis2.in :homing @01:08:08.70 Archive.in :homing @01:08:08.74 PinPort.in :homing @01:08:08.75 Air.in :homing @01:08:08.81 Toroid.in :homing @01:08:08.96 Lysis1.jog 1604 @01:08:09.11 Archive.jog 300 @01:08:09.14 Lysis2.jog 1621 @01:08:09.19 Air.jog 1431 @01:08:09.22 PinPort.jog 1239 @01:08:09.37 Toroid.jog 1366 @01:08:09.71 Plunge.setRawPosition! 50 @01:08:09.80 Plunge.seek :home @01:08:09.91 Archive.setRawPosition! 50 @01:08:09.98 Archive.seek :home @01:08:10.09 Plunge.in :default @01:08:10.27 Archive.in :default @01:08:10.54 Lysis1.setRawPosition! 50 @01:08:10.60 Lysis1.seek :home @01:08:10.72 Lysis2.setRawPosition! 50 @01:08:10.75 Air.setRawPosition! 0 @01:08:11.29 Lysis2.seek :home @01:08:11.40 Air.seek :home @01:08:11.62 PinPort.setRawPosition! 3150 @01:08:11.69 Lysis1.in :default @01:08:11.73 Air.in :default @01:08:11.78 PinPort.seek :home @01:08:12.00 Lysis2.in :default @01:08:14.07 Toroid.setRawPosition! 0 @01:08:14.12 Toroid.seek :home @01:08:14.21 Toroid.in :default @01:08:16.24 PinPort.in :default @01:08:16.58 Toroid.seek :clear @01:08:16.73 PinPort.in :unlocking @01:08:16.79 PinPort.seek :clear @01:08:20.24 Air.seek :clear @01:08:20.33 Lysis1.seek :clear @01:08:21.06 Lysis2.seek :clear @01:08:21.84 Archive.seek :clear @01:08:22.62 Carousel.in :default @01:08:22.74 Carousel.home.seek 42 @01:08:23.00 Selecting Cartridge 36 @01:08:23.29 Carousel.seek 36 @01:08:24.87 Carousel.reconfigure :align @01:08:24.99 Lysis1.in :fast @01:08:25.05 Lysis1.seek :locked @01:08:25.13 Slide::Warning in core -- Storage Carousel (SC) reseek at 36 + 4 counts @01:08:27.67 Plunge.seek :locked @01:08:27.88 Air.in :fast @01:08:27.94 Air.seek :locked @01:08:29.00 Carousel.reconfigure :locked @01:08:29.16 Powering cartridge bus @01:08:31.85 Gate.setIR 1750,5000,0,6 @01:08:31.97 Gate.setIR 0,5000,0,6 @01:08:32.04 PuckHeater.configure :default @01:08:32.11 Gate.power :heat,:ON,limit to 5000mA @01:08:32.27 Mounted lysis Cartridge board#J59925 @01:08:32.31 Using default cartridge heat loss curve for HeatingFunctionInCarousel @01:08:33.83 SPR.command "read_sequences" @01:08:33.97 SPR.command "stats_on" @01:08:34.71 SPR.command "start 0 1" @01:08:34.84 SPR.start /var/log/esp/spr26jul2901h.spr.bz2 @01:08:36.83 Waiting for baseline SPR stabilization @01:08:36.85 Handoff.select :waste @01:08:37.35 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:08:44.01 Syringe tare is 50% of full scale at 22C (drifted -1%) @01:08:44.04 PinPort.in :locking @01:08:44.09 PinPort.seek :locked @01:08:44.96 Archive.in :fast @01:08:45.05 Lysis2.in :fast @01:08:45.09 Lysis1.seek 1525uL @01:08:45.16 Archive.seek :empty @01:08:45.27 Lysis2.seek 1525uL @01:08:53.67 delayUntil "01:18:36.40" @01:18:36.41 Lysis2.in :DAmix @01:18:36.52 Lysis1.in :DAmix @01:18:37.16 Lysis2.seek 1275uL @01:18:37.18 Lysis1.seek 1275uL @01:19:12.16 delay 5 seconds @01:19:17.18 Handoff.select SPR @01:19:17.68 SPR.command "start 2 14" @01:19:19.57 SPR stabilized @01:19:19.72 delay 20 seconds @01:19:39.74 SPR.command "start 2 0" @01:19:43.35 Waiting up to 11:00 for SPR to enter state 1 @01:21:41.45 SPR.command "hold_on" @01:21:42.82 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:21:43.46 Lysis2.seek 975uL @01:21:43.52 Lysis1.seek 975uL @01:22:25.43 delay 15 seconds @01:22:40.45 SPR.command "hold_off" @01:22:43.48 Waiting up to 1:00:00 for SPR to enter state 7 @01:38:59.05 SPRsummary:4.67RIU,255RIU,252RIU,363RIU after 1926s @01:53:16.08 delay 20 seconds @01:53:36.11 SPR.command "start 2 0" @01:53:39.72 Waiting up to 11:00 for SPR to enter state 1 @01:55:37.83 SPR.command "hold_on" @01:55:39.20 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:55:39.95 Lysis2.seek 825uL @01:55:40.01 Lysis1.seek 825uL @01:56:01.06 delay 15 seconds @01:56:16.08 SPR.command "hold_off" @01:56:19.04 Waiting up to 1:00:00 for SPR to enter state 7 @02:26:51.52 SPR analysis completed @02:26:52.11 Ended log spr26jul2901h.spr.bz2 @02:26:52.13 SPR.command "stats_off" @02:26:53.45 Handoff.select :close @02:26:53.96 Gate.power :heat,:OFF @02:26:53.98 Gate.power :cartridge,:OFF @02:26:54.07 PinPort.in :unlocking @02:26:54.12 PinPort.seek :clear @02:26:54.94 Carousel.in :default @02:26:55.06 Carousel.home.seek 36 @02:26:55.40 Plunge.seek :locked @02:26:55.58 Archive.in :unlocking @02:26:55.71 Archive.seek :clear @02:26:55.85 Air.in :unlocking @02:26:56.00 Air.seek :clear @02:26:56.05 Lysis1.in :unlocking @02:26:56.71 Lysis1.seek :clear @02:26:56.85 Lysis2.in :unlocking @02:26:56.91 Lysis2.seek :clear @02:27:00.51 Gate.power :motor,:OFF @02:27:00.53 Gate.power :analytic1,:OFF @02:27:00.54 Gate.power :core,:OFF @02:27:00.56 Gate.power :main,:OFF @02:27:00.57 Safely stopped and ready to power off @02:27:00.78 Gate.power :sampler,:OFF,limit to 3500mA @02:27:00.80 Gate.power :skimmer,:OFF,limit to 3500mA @02:27:04.37 Gate.power :main,:ON,limit to 5000mA @02:27:04.39 Gate.power :analytic1,:ON,limit to 4000mA @02:27:04.46 Powering core bus @02:27:06.83 SPR Module 1, FW Rev. B, FPGA Rev. C @02:27:07.25 Plunge.configure :default @02:27:07.34 SamplePump.configure :default @02:27:07.42 Toroid.configure :default @02:27:07.50 Carousel.configure :default @02:27:07.58 Abs.configure :default @02:27:07.66 PhaseSeparator.configure :default @02:27:07.75 Archive.configure :default @02:27:07.86 Air.configure :default @02:27:07.92 Lysis1.configure :default @02:27:07.97 Lysis2.configure :default @02:27:08.05 PinPort.configure :default @02:27:08.11 All elf controllers running firmware version 4.94 @02:27:08.13 Gate.power :motor,:ON,limit to 5000mA @02:27:08.51 Plunge.home.jog 0 @02:27:09.05 Lysis1.home.jog 0 @02:27:09.19 Lysis2.home.jog 0 @02:27:09.24 Archive.home.jog 0 @02:27:09.27 Carousel.home.jog 0 @02:27:09.30 PinPort.home.jog 0 @02:27:09.33 Air.home.jog 0 @02:27:09.36 Toroid.home.jog 0 @02:27:10.41 Plunge.in :homing @02:27:10.65 Plunge.jog 1483 @02:27:10.71 PinPort.in :homing @02:27:10.75 Lysis1.in :homing @02:27:10.77 Archive.in :homing @02:27:10.79 Lysis2.in :homing @02:27:10.81 Toroid.in :homing @02:27:10.84 Air.in :homing @02:27:11.18 PinPort.jog 1250 @02:27:11.27 Lysis1.jog 276 @02:27:11.29 Archive.jog 300 @02:27:11.32 Toroid.jog 1365 @02:27:11.35 Air.jog 1433 @02:27:11.39 Lysis2.jog 1621 @02:27:12.08 Lysis1.setRawPosition! 50 @02:27:12.18 Archive.setRawPosition! 50 @02:27:12.21 Plunge.setRawPosition! 50 @02:27:12.24 Lysis1.seek :home @02:27:12.89 Archive.seek :home @02:27:13.03 Plunge.seek :home @02:27:13.24 Lysis2.setRawPosition! 50 @02:27:13.28 Lysis1.in :default @02:27:13.30 Air.setRawPosition! 0 @02:27:13.43 Lysis2.seek :home @02:27:13.51 Air.seek :home @02:27:13.74 PinPort.setRawPosition! 3150 @02:27:13.83 Air.in :default @02:27:13.86 Plunge.in :default @02:27:13.93 Archive.in :default @02:27:14.04 PinPort.seek :home @02:27:14.20 Lysis2.in :default @02:27:16.07 Toroid.setRawPosition! 0 @02:27:16.13 Toroid.seek :home @02:27:16.21 Toroid.in :default @02:27:18.47 PinPort.in :default @02:27:18.81 Toroid.seek :clear @02:27:18.97 PinPort.in :unlocking @02:27:19.02 PinPort.seek :clear @02:27:22.47 Air.seek :clear @02:27:22.56 Lysis1.seek :clear @02:27:23.28 Lysis2.seek :clear @02:27:24.08 Archive.seek :clear @02:27:24.86 Carousel.in :default @02:27:24.98 Carousel.home.seek 36 @02:27:25.25 Selecting Cartridge 30 @02:27:25.54 Carousel.seek 30 @02:27:27.12 Carousel.reconfigure :align @02:27:27.24 Lysis1.in :fast @02:27:27.30 Lysis1.seek :locked @02:27:29.93 Plunge.seek :locked @02:27:30.09 Air.in :fast @02:27:30.15 Air.seek :locked @02:27:31.22 Carousel.reconfigure :locked @02:27:31.37 Powering cartridge bus @02:27:34.05 Gate.setIR 1750,5000,0,6 @02:27:34.18 Gate.setIR 0,5000,0,6 @02:27:34.25 PuckHeater.configure :default @02:27:34.32 Gate.power :heat,:ON,limit to 5000mA @02:27:34.49 Mounted lysis Cartridge board#J59986 @02:27:34.52 Using default cartridge heat loss curve for HeatingFunctionInCarousel @02:27:36.60 SPR.command "read_sequences" @02:27:36.98 SPR.command "stats_on" @02:27:37.15 SPR.command "start 0 1" @02:27:37.85 SPR.start /var/log/esp/spr26jul2902h.spr.bz2 @02:27:39.83 Waiting for baseline SPR stabilization @02:27:39.86 Handoff.select :waste @02:27:40.37 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:27:49.50 Syringe tare is 50% of full scale at 22C (drifted -1%) @02:27:49.52 PinPort.in :locking @02:27:49.57 PinPort.seek :locked @02:27:50.42 Archive.in :fast @02:27:50.50 Lysis2.in :fast @02:27:50.55 Lysis1.seek 1525uL @02:27:50.62 Archive.seek :empty @02:27:50.77 Lysis2.seek 1525uL @02:27:59.12 delayUntil "02:37:39.40" @02:37:39.42 Lysis2.in :DAmix @02:37:39.55 Lysis1.in :DAmix @02:37:40.09 Lysis2.seek 1275uL @02:37:40.15 Lysis1.seek 1275uL @02:38:15.09 delay 5 seconds @02:38:20.12 Handoff.select SPR @02:38:20.61 SPR.command "start 2 14" @02:38:21.11 SPR stabilized @02:38:21.26 delay 20 seconds @02:38:41.28 SPR.command "start 2 0" @02:38:43.94 Waiting up to 11:00 for SPR to enter state 1 @02:40:41.60 SPR.command "hold_on" @02:40:42.99 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @02:40:43.65 Lysis2.seek 975uL @02:40:43.70 Lysis1.seek 975uL @02:41:25.66 delay 15 seconds @02:41:40.68 SPR.command "hold_off" @02:41:43.63 Waiting up to 1:00:00 for SPR to enter state 7 @02:57:59.21 SPRsummary:4.01RIU,156RIU,141RIU,211RIU after 1923s @03:12:16.26 delay 20 seconds @03:12:36.28 SPR.command "start 2 0" @03:12:39.89 Waiting up to 11:00 for SPR to enter state 1 @03:14:37.98 SPR.command "hold_on" @03:14:39.37 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @03:14:40.13 Lysis2.seek 825uL @03:14:40.17 Lysis1.seek 825uL @03:15:01.23 delay 15 seconds @03:15:16.25 SPR.command "hold_off" @03:15:19.21 Waiting up to 1:00:00 for SPR to enter state 7 @03:45:51.70 SPR analysis completed @03:45:52.29 Ended log spr26jul2902h.spr.bz2 @03:45:52.31 SPR.command "stats_off" @03:45:53.61 Handoff.select :close @03:45:54.14 Gate.power :heat,:OFF @03:45:54.15 Gate.power :cartridge,:OFF @03:45:54.23 PinPort.in :unlocking @03:45:54.29 PinPort.seek :clear @03:45:55.11 Carousel.in :default @03:45:55.23 Carousel.home.seek 30 @03:45:55.56 Plunge.seek :locked @03:45:55.74 Archive.in :unlocking @03:45:55.91 Archive.seek :clear @03:45:56.02 Air.in :unlocking @03:45:56.13 Air.seek :clear @03:45:56.25 Lysis1.in :unlocking @03:45:56.39 Lysis1.seek :clear @03:45:56.45 Lysis2.in :unlocking @03:45:56.56 Lysis2.seek :clear @03:46:01.38 Gate.power :motor,:OFF @03:46:01.42 Gate.power :analytic1,:OFF @03:46:01.43 Gate.power :core,:OFF @03:46:01.44 Gate.power :main,:OFF @03:46:01.46 Safely stopped and ready to power off @03:46:01.83 Gate.power :sampler,:OFF,limit to 3500mA @03:46:01.85 Gate.power :skimmer,:OFF,limit to 3500mA @03:46:05.26 Gate.power :main,:ON,limit to 5000mA @03:46:05.27 Gate.power :analytic1,:ON,limit to 4000mA @03:46:05.35 Powering core bus @03:46:07.71 SPR Module 1, FW Rev. B, FPGA Rev. C @03:46:08.14 Plunge.configure :default @03:46:08.22 SamplePump.configure :default @03:46:08.31 Toroid.configure :default @03:46:08.39 Carousel.configure :default @03:46:08.47 Abs.configure :default @03:46:08.55 PhaseSeparator.configure :default @03:46:08.63 Archive.configure :default @03:46:08.71 Air.configure :default @03:46:08.77 Lysis1.configure :default @03:46:08.82 Lysis2.configure :default @03:46:08.91 PinPort.configure :default @03:46:08.96 All elf controllers running firmware version 4.94 @03:46:08.98 Gate.power :motor,:ON,limit to 5000mA @03:46:09.49 Plunge.home.jog 0 @03:46:09.88 PinPort.home.jog 0 @03:46:10.02 Lysis1.home.jog 0 @03:46:10.06 Air.home.jog 0 @03:46:10.09 Carousel.home.jog 0 @03:46:10.17 Archive.home.jog 0 @03:46:10.26 Toroid.home.jog 0 @03:46:10.30 Lysis2.home.jog 0 @03:46:11.11 Plunge.in :homing @03:46:11.36 Plunge.jog 1486 @03:46:11.45 Lysis2.in :homing @03:46:11.48 Air.in :homing @03:46:11.52 Toroid.in :homing @03:46:11.54 PinPort.in :homing @03:46:11.56 Lysis1.in :homing @03:46:11.59 Archive.in :homing @03:46:11.93 Air.jog 1432 @03:46:11.97 Lysis2.jog 1620 @03:46:12.02 PinPort.jog 1258 @03:46:12.04 Lysis1.jog 1605 @03:46:12.06 Toroid.jog 1363 @03:46:12.09 Archive.jog 1626 @03:46:12.86 Plunge.setRawPosition! 50 @03:46:12.91 Plunge.seek :home @03:46:13.19 Plunge.in :default @03:46:13.33 Air.setRawPosition! 0 @03:46:13.38 Air.seek :home @03:46:13.50 Lysis2.setRawPosition! 50 @03:46:13.52 Air.in :default @03:46:13.63 Lysis2.seek :home @03:46:13.81 Archive.setRawPosition! 50 @03:46:13.83 Lysis1.setRawPosition! 50 @03:46:14.42 Lysis1.seek :home @03:46:14.55 Archive.seek :home @03:46:14.74 PinPort.setRawPosition! 3150 @03:46:14.77 Lysis2.in :default @03:46:14.86 PinPort.seek :home @03:46:15.02 Lysis1.in :default @03:46:15.04 Archive.in :default @03:46:16.89 Toroid.setRawPosition! 0 @03:46:16.94 Toroid.seek :home @03:46:17.02 Toroid.in :default @03:46:19.27 PinPort.in :default @03:46:19.61 Toroid.seek :clear @03:46:19.77 PinPort.in :unlocking @03:46:19.85 PinPort.seek :clear @03:46:23.21 Air.seek :clear @03:46:23.30 Lysis1.seek :clear @03:46:24.03 Lysis2.seek :clear @03:46:24.81 Archive.seek :clear @03:46:25.59 Carousel.in :default @03:46:25.71 Carousel.home.seek 30 @03:46:25.97 Selecting Cartridge 24 @03:46:26.27 Carousel.seek 24 @03:46:27.88 Carousel.reconfigure :align @03:46:28.00 Lysis1.in :fast @03:46:28.05 Lysis1.seek :locked @03:46:30.68 Plunge.seek :locked @03:46:30.84 Air.in :fast @03:46:30.90 Air.seek :locked @03:46:31.97 Carousel.reconfigure :locked @03:46:32.12 Powering cartridge bus @03:46:34.79 Gate.setIR 1750,5000,0,6 @03:46:34.92 Gate.setIR 0,5000,0,6 @03:46:34.98 PuckHeater.configure :default @03:46:35.05 Gate.power :heat,:ON,limit to 5000mA @03:46:35.21 Mounted lysis Cartridge board#J59995 @03:46:35.25 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:46:36.84 SPR.command "read_sequences" @03:46:36.92 SPR.command "stats_on" @03:46:37.66 SPR.command "start 0 1" @03:46:37.79 SPR.start /var/log/esp/spr26jul2903h.spr.bz2 @03:46:39.80 Waiting for baseline SPR stabilization @03:46:39.83 Handoff.select :waste @03:46:40.32 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:46:53.44 Syringe tare is 50% of full scale at 22C (drifted -1%) @03:46:53.47 PinPort.in :locking @03:46:53.57 PinPort.seek :locked @03:46:54.40 Archive.in :fast @03:46:54.48 Lysis2.in :fast @03:46:54.56 Lysis1.seek 1525uL @03:46:54.69 Archive.seek :empty @03:46:54.78 Lysis2.seek 1525uL @03:47:03.14 delayUntil "03:56:39.35" @03:56:39.50 Lysis2.in :DAmix @03:56:39.61 Lysis1.in :DAmix @03:56:40.19 Lysis2.seek 1275uL @03:56:40.21 Lysis1.seek 1275uL @03:57:15.18 delay 5 seconds @03:57:20.20 Handoff.select SPR @03:57:20.69 SPR.command "start 2 14" @03:57:22.52 SPR stabilized @03:57:22.67 delay 20 seconds @03:57:42.69 SPR.command "start 2 0" @03:57:45.80 Waiting up to 11:00 for SPR to enter state 1 @03:59:43.45 SPR.command "hold_on" @03:59:45.35 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @03:59:46.03 Lysis2.seek 975uL @03:59:46.08 Lysis1.seek 975uL @04:00:27.99 delay 15 seconds @04:00:43.02 SPR.command "hold_off" @04:00:46.42 Waiting up to 1:00:00 for SPR to enter state 7 @04:17:01.06 SPRsummary:3.82RIU,42.8RIU,46.4RIU,65.9RIU after 1925s @04:31:18.09 delay 20 seconds @04:31:38.12 SPR.command "start 2 0" @04:31:41.21 Waiting up to 11:00 for SPR to enter state 1 @04:33:38.89 SPR.command "hold_on" @04:33:40.27 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @04:33:40.97 Lysis2.seek 825uL @04:33:41.03 Lysis1.seek 825uL @04:34:02.08 delay 15 seconds @04:34:17.10 SPR.command "hold_off" @04:34:20.11 Waiting up to 1:00:00 for SPR to enter state 7 @05:04:52.59 SPR analysis completed @05:04:53.13 Ended log spr26jul2903h.spr.bz2 @05:04:53.15 SPR.command "stats_off" @05:04:54.56 Handoff.select :close @05:04:55.07 Gate.power :heat,:OFF @05:04:55.09 Gate.power :cartridge,:OFF @05:04:55.17 PinPort.in :unlocking @05:04:55.22 PinPort.seek :clear @05:04:56.05 Carousel.in :default @05:04:56.17 Carousel.home.seek 24 @05:04:56.51 Plunge.seek :locked @05:04:56.68 Archive.in :unlocking @05:04:56.81 Archive.seek :clear @05:04:56.88 Air.in :unlocking @05:04:57.04 Air.seek :clear @05:04:57.15 Lysis1.in :unlocking @05:04:57.26 Lysis1.seek :clear @05:04:57.38 Lysis2.in :unlocking @05:04:57.44 Lysis2.seek :clear @05:05:01.74 Gate.power :motor,:OFF @05:05:01.78 Gate.power :analytic1,:OFF @05:05:01.81 Gate.power :core,:OFF @05:05:01.82 Gate.power :main,:OFF @05:05:01.84 Safely stopped and ready to power off @05:05:02.05 Gate.power :sampler,:OFF,limit to 3500mA @05:05:02.07 Gate.power :skimmer,:OFF,limit to 3500mA @05:05:05.64 Gate.power :main,:ON,limit to 5000mA @05:05:05.65 Gate.power :analytic1,:ON,limit to 4000mA @05:05:05.73 Powering core bus @05:05:08.08 SPR Module 1, FW Rev. B, FPGA Rev. C @05:05:08.52 Plunge.configure :default @05:05:08.61 SamplePump.configure :default @05:05:08.69 Toroid.configure :default @05:05:08.77 Carousel.configure :default @05:05:08.85 Abs.configure :default @05:05:08.94 PhaseSeparator.configure :default @05:05:09.02 Archive.configure :default @05:05:09.10 Air.configure :default @05:05:09.15 Lysis1.configure :default @05:05:09.21 Lysis2.configure :default @05:05:09.29 PinPort.configure :default @05:05:09.34 All elf controllers running firmware version 4.94 @05:05:09.37 Gate.power :motor,:ON,limit to 5000mA @05:05:09.57 Plunge.home.jog 0 @05:05:10.39 PinPort.home.jog 0 @05:05:10.43 Lysis1.home.jog 0 @05:05:10.46 Air.home.jog 0 @05:05:10.50 Archive.home.jog 0 @05:05:10.53 Toroid.home.jog 0 @05:05:10.55 Lysis2.home.jog 0 @05:05:10.57 Carousel.home.jog 0 @05:05:11.25 Plunge.in :homing @05:05:11.52 Plunge.jog 1493 @05:05:11.73 Lysis2.in :homing @05:05:11.82 Lysis1.in :homing @05:05:11.85 Archive.in :homing @05:05:11.87 PinPort.in :homing @05:05:11.89 Air.in :homing @05:05:11.91 Toroid.in :homing @05:05:12.16 Lysis2.jog 1621 @05:05:12.20 Lysis1.jog 1605 @05:05:12.25 Archive.jog 301 @05:05:12.28 PinPort.jog 1249 @05:05:12.39 Toroid.jog 1355 @05:05:12.57 Air.jog 1431 @05:05:12.85 Plunge.setRawPosition! 50 @05:05:12.90 Plunge.seek :home @05:05:13.13 Archive.setRawPosition! 50 @05:05:13.23 Plunge.in :default @05:05:13.24 Archive.seek :home @05:05:13.56 Archive.in :default @05:05:13.65 Lysis2.setRawPosition! 50 @05:05:13.71 Lysis2.seek :home @05:05:13.85 Lysis1.setRawPosition! 50 @05:05:14.52 PinPort.setRawPosition! 3150 @05:05:14.58 Lysis2.in :default @05:05:14.59 Air.setRawPosition! 0 @05:05:14.62 Lysis1.seek :home @05:05:14.82 PinPort.seek :home @05:05:14.89 Air.seek :home @05:05:15.10 Air.in :default @05:05:15.12 Lysis1.in :default @05:05:17.19 Toroid.setRawPosition! 0 @05:05:17.24 Toroid.seek :home @05:05:17.33 Toroid.in :default @05:05:19.31 PinPort.in :default @05:05:19.65 Toroid.seek :clear @05:05:19.86 PinPort.in :unlocking @05:05:19.92 PinPort.seek :clear @05:05:23.26 Air.seek :clear @05:05:23.36 Lysis1.seek :clear @05:05:24.08 Lysis2.seek :clear @05:05:24.87 Archive.seek :clear @05:05:25.65 Carousel.in :default @05:05:25.79 Carousel.home.seek 24 @05:05:26.08 Selecting Cartridge 17 @05:05:26.37 Carousel.seek 17 @05:05:28.15 Carousel.reconfigure :align @05:05:28.27 Lysis1.in :fast @05:05:28.32 Lysis1.seek :locked @05:05:28.57 Slide::Warning in core -- Storage Carousel (SC) reseek at 17 + 3 counts (sensed 17 + 4 counts) @05:05:30.94 Plunge.seek :locked @05:05:31.11 Air.in :fast @05:05:31.16 Air.seek :locked @05:05:32.23 Carousel.reconfigure :locked @05:05:32.38 Powering cartridge bus @05:05:35.05 Gate.setIR 1750,5000,0,6 @05:05:35.17 Gate.setIR 0,5000,0,6 @05:05:35.24 PuckHeater.configure :default @05:05:35.30 Gate.power :heat,:ON,limit to 5000mA @05:05:35.46 Mounted lysis Cartridge board#J59993 @05:05:35.50 Using default cartridge heat loss curve for HeatingFunctionInCarousel @05:05:37.06 SPR.command "read_sequences" @05:05:37.61 SPR.command "stats_on" @05:05:38.35 SPR.command "start 0 1" @05:05:39.06 SPR.start /var/log/esp/spr26jul2905h.spr.bz2 @05:05:41.63 Waiting for baseline SPR stabilization @05:05:41.64 Handoff.select :waste @05:05:42.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:05:50.26 Syringe tare is 50% of full scale at 23C (drifted -1%) @05:05:50.29 PinPort.in :locking @05:05:50.34 PinPort.seek :locked @05:05:51.17 Archive.in :fast @05:05:51.25 Lysis2.in :fast @05:05:51.32 Lysis1.seek 1525uL @05:05:51.35 Archive.seek :empty @05:05:51.50 Lysis2.seek 1525uL @05:05:59.80 delayUntil "05:15:41.59" @05:15:41.61 Lysis2.in :DAmix @05:15:41.72 Lysis1.in :DAmix @05:15:42.39 Lysis2.seek 1275uL @05:15:42.45 Lysis1.seek 1275uL @05:16:17.42 delay 5 seconds @05:16:22.44 Handoff.select SPR @05:16:22.92 SPR.command "start 2 14" @05:16:24.27 SPR stabilized @05:16:24.41 delay 20 seconds @05:16:44.43 SPR.command "start 2 0" @05:16:47.10 Waiting up to 11:00 for SPR to enter state 1 @05:18:44.77 SPR.command "hold_on" @05:18:46.15 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:18:46.91 Lysis2.seek 975uL @05:18:46.96 Lysis1.seek 975uL @05:19:28.91 delay 15 seconds @05:19:43.93 SPR.command "hold_off" @05:19:46.80 Waiting up to 1:00:00 for SPR to enter state 7 @05:36:02.37 SPRsummary:4.46RIU,21.2RIU,25RIU,37.3RIU after 1925s @05:50:19.42 delay 20 seconds @05:50:39.44 SPR.command "start 2 0" @05:50:42.53 Waiting up to 11:00 for SPR to enter state 1 @05:52:40.20 SPR.command "hold_on" @05:52:41.59 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:52:42.21 Lysis2.seek 825uL @05:52:42.27 Lysis1.seek 825uL @05:53:03.33 delay 15 seconds @05:53:18.35 SPR.command "hold_off" @05:53:21.43 Waiting up to 1:00:00 for SPR to enter state 7 @06:23:53.91 SPR analysis completed @06:23:54.44 Ended log spr26jul2905h.spr.bz2 @06:23:54.46 SPR.command "stats_off" @06:23:55.92 Handoff.select :close @06:23:56.43 Gate.power :heat,:OFF @06:23:56.44 Gate.power :cartridge,:OFF @06:23:56.52 PinPort.in :unlocking @06:23:56.58 PinPort.seek :clear @06:23:57.40 Carousel.in :default @06:23:57.52 Carousel.home.seek 17 @06:23:57.88 Plunge.seek :locked @06:23:58.07 Archive.in :unlocking @06:23:58.19 Archive.seek :clear @06:23:58.29 Air.in :unlocking @06:23:58.41 Air.seek :clear @06:23:58.52 Lysis1.in :unlocking @06:23:58.65 Lysis1.seek :clear @06:23:59.26 Lysis2.in :unlocking @06:23:59.32 Lysis2.seek :clear @06:24:03.00 Gate.power :motor,:OFF @06:24:03.02 Gate.power :analytic1,:OFF @06:24:03.03 Gate.power :core,:OFF @06:24:03.04 Gate.power :main,:OFF @06:24:03.06 Safely stopped and ready to power off @06:24:03.63 Gate.power :sampler,:OFF,limit to 3500mA @06:24:03.65 Gate.power :skimmer,:OFF,limit to 3500mA @06:24:06.85 Gate.power :main,:ON,limit to 5000mA @06:24:06.87 Gate.power :analytic1,:ON,limit to 4000mA @06:24:06.95 Powering core bus @06:24:09.30 SPR Module 1, FW Rev. B, FPGA Rev. C @06:24:09.79 Plunge.configure :default @06:24:09.90 SamplePump.configure :default @06:24:09.98 Toroid.configure :default @06:24:10.06 Carousel.configure :default @06:24:10.14 Abs.configure :default @06:24:10.22 PhaseSeparator.configure :default @06:24:10.31 Archive.configure :default @06:24:10.38 Air.configure :default @06:24:10.44 Lysis1.configure :default @06:24:10.50 Lysis2.configure :default @06:24:10.58 PinPort.configure :default @06:24:10.63 All elf controllers running firmware version 4.94 @06:24:10.66 Gate.power :motor,:ON,limit to 5000mA @06:24:10.98 Toroid.home.jog 0 @06:24:11.24 Plunge.home.jog 0 @06:24:11.85 Lysis2.home.jog 0 @06:24:11.88 Air.home.jog 0 @06:24:11.92 Archive.home.jog 0 @06:24:12.00 Carousel.home.jog 0 @06:24:12.04 PinPort.home.jog 0 @06:24:12.09 Lysis1.home.jog 0 @06:24:12.88 Toroid.in :homing @06:24:12.96 Plunge.in :homing @06:24:13.21 Toroid.jog 1356 @06:24:13.28 Plunge.jog 1487 @06:24:13.31 Lysis2.in :homing @06:24:13.36 Air.in :homing @06:24:13.38 Lysis1.in :homing @06:24:13.40 Archive.in :homing @06:24:13.44 PinPort.in :homing @06:24:13.71 Lysis2.jog 1620 @06:24:13.75 Air.jog 1431 @06:24:13.99 Archive.jog 302 @06:24:14.05 PinPort.jog 1244 @06:24:14.09 Lysis1.jog 1604 @06:24:14.72 Archive.setRawPosition! 50 @06:24:14.77 Archive.seek :home @06:24:14.87 Plunge.setRawPosition! 50 @06:24:14.93 Plunge.seek :home @06:24:15.56 Lysis1.setRawPosition! 50 @06:24:15.70 Air.setRawPosition! 0 @06:24:15.72 Lysis2.setRawPosition! 50 @06:24:15.73 Archive.in :default @06:24:15.77 Plunge.in :default @06:24:16.07 Lysis1.seek :home @06:24:16.10 Lysis2.seek :home @06:24:16.14 Air.seek :home @06:24:16.45 PinPort.setRawPosition! 3150 @06:24:16.48 Air.in :default @06:24:16.60 PinPort.seek :home @06:24:16.73 Lysis1.in :default @06:24:16.75 Lysis2.in :default @06:24:17.95 Toroid.setRawPosition! 0 @06:24:18.00 Toroid.seek :home @06:24:18.08 Toroid.in :default @06:24:20.96 PinPort.in :default @06:24:21.30 Toroid.seek :clear @06:24:21.46 PinPort.in :unlocking @06:24:21.52 PinPort.seek :clear @06:24:24.97 Air.seek :clear @06:24:25.06 Lysis1.seek :clear @06:24:25.80 Lysis2.seek :clear @06:24:26.60 Archive.seek :clear @06:24:27.38 Carousel.in :default @06:24:27.50 Carousel.home.seek 17 @06:24:27.76 Selecting Cartridge 12 @06:24:28.10 Carousel.seek 12 @06:24:29.47 Carousel.reconfigure :align @06:24:29.60 Lysis1.in :fast @06:24:29.65 Lysis1.seek :locked @06:24:32.29 Plunge.seek :locked @06:24:32.45 Air.in :fast @06:24:32.50 Air.seek :locked @06:24:33.57 Carousel.reconfigure :locked @06:24:33.73 Powering cartridge bus @06:24:36.40 Gate.setIR 1750,5000,0,6 @06:24:36.53 Gate.setIR 0,5000,0,6 @06:24:36.59 PuckHeater.configure :default @06:24:36.66 Gate.power :heat,:ON,limit to 5000mA @06:24:36.82 Mounted lysis Cartridge board#J59971 @06:24:36.86 Using default cartridge heat loss curve for HeatingFunctionInCarousel @06:24:38.99 SPR.command "read_sequences" @06:24:39.47 SPR.command "stats_on" @06:24:39.64 SPR.command "start 0 1" @06:24:40.34 SPR.start /var/log/esp/spr26jul2906h.spr.bz2 @06:24:42.32 Waiting for baseline SPR stabilization @06:24:42.34 Handoff.select :waste @06:24:42.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @06:24:49.51 Syringe tare is 50% of full scale at 23C (drifted -0%) @06:24:49.53 PinPort.in :locking @06:24:49.58 PinPort.seek :locked @06:24:50.44 Archive.in :fast @06:24:50.50 Lysis2.in :fast @06:24:50.57 Lysis1.seek 1525uL @06:24:50.72 Archive.seek :empty @06:24:50.78 Lysis2.seek 1525uL @06:24:59.18 delayUntil "06:34:41.89" @06:32:25.11 Session Begun @06:32:27.27 -> slot {6 => { Type: :microSPR_phyto, State: :standard, lysed: 1525, lysed2: 1525}, 12 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: spr26jul2906h.spr.bz2}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2905h.spr.bz2}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2903h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2902h.spr.bz2}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2901h.spr.bz2}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2823h.spr.bz2}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2822h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2821h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: spr26jul2819h.spr.bz2}} @06:34:41.90 Lysis2.in :DAmix @06:34:42.06 Lysis1.in :DAmix @06:34:42.75 Lysis2.seek 1275uL @06:34:42.80 Lysis1.seek 1275uL @06:35:17.86 delay 5 seconds @06:35:22.88 Handoff.select SPR @06:35:23.40 SPR.command "start 2 14" @06:35:24.54 SPR stabilized @06:35:24.74 delay 20 seconds @06:35:44.76 SPR.command "start 2 0" @06:35:47.37 Waiting up to 11:00 for SPR to enter state 1 @06:37:45.04 SPR.command "hold_on" @06:37:46.95 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @06:37:47.62 Lysis2.seek 975uL @06:37:47.73 Lysis1.seek 975uL @06:38:29.68 delay 15 seconds @06:38:44.70 SPR.command "hold_off" @06:38:48.55 Waiting up to 1:00:00 for SPR to enter state 7 @06:55:03.59 SPRsummary:5.06RIU,14.7RIU,12.4RIU,17.6RIU after 1925s @07:09:20.65 delay 20 seconds @07:09:40.67 SPR.command "start 2 0" @07:09:43.75 Waiting up to 11:00 for SPR to enter state 1 @07:11:41.42 SPR.command "hold_on" @07:11:42.80 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @07:11:43.53 Lysis2.seek 825uL @07:11:43.60 Lysis1.seek 825uL @07:12:04.69 delay 15 seconds @07:12:19.72 SPR.command "hold_off" @07:12:22.65 Waiting up to 1:00:00 for SPR to enter state 7 @07:42:45.45 Session Ended @07:42:55.13 SPR analysis completed @07:42:55.67 Ended log spr26jul2906h.spr.bz2 @07:42:55.69 SPR.command "stats_off" @07:42:57.10 Handoff.select :close @07:42:57.61 Gate.power :heat,:OFF @07:42:57.63 Gate.power :cartridge,:OFF @07:42:57.71 PinPort.in :unlocking @07:42:57.76 PinPort.seek :clear @07:42:58.61 Carousel.in :default @07:42:58.73 Carousel.home.seek 12 @07:42:59.06 Plunge.seek :locked @07:42:59.24 Archive.in :unlocking @07:42:59.35 Archive.seek :clear @07:42:59.46 Air.in :unlocking @07:42:59.57 Air.seek :clear @07:42:59.69 Lysis1.in :unlocking @07:42:59.86 Lysis1.seek :clear @07:42:59.97 Lysis2.in :unlocking @07:43:00.04 Lysis2.seek :clear @07:43:04.16 Gate.power :motor,:OFF @07:43:04.18 Gate.power :analytic1,:OFF @07:43:04.19 Gate.power :core,:OFF @07:43:04.20 Gate.power :main,:OFF @07:43:04.22 Safely stopped and ready to power off @07:43:04.79 Gate.power :sampler,:OFF,limit to 3500mA @07:43:04.81 Gate.power :skimmer,:OFF,limit to 3500mA @07:43:08.01 Gate.power :main,:ON,limit to 5000mA @07:43:08.03 Gate.power :analytic1,:ON,limit to 4000mA @07:43:08.10 Powering core bus @07:43:10.46 SPR Module 1, FW Rev. B, FPGA Rev. C @07:43:10.88 Plunge.configure :default @07:43:10.97 SamplePump.configure :default @07:43:11.05 Toroid.configure :default @07:43:11.13 Carousel.configure :default @07:43:11.21 Abs.configure :default @07:43:11.90 PhaseSeparator.configure :default @07:43:11.98 Archive.configure :default @07:43:12.06 Air.configure :default @07:43:12.11 Lysis1.configure :default @07:43:12.17 Lysis2.configure :default @07:43:12.25 PinPort.configure :default @07:43:12.30 All elf controllers running firmware version 4.94 @07:43:12.33 Gate.power :motor,:ON,limit to 5000mA @07:43:12.55 Plunge.home.jog 0 @07:43:13.30 Lysis2.home.jog 0 @07:43:13.34 Air.home.jog 0 @07:43:13.39 Carousel.home.jog 0 @07:43:13.43 Lysis1.home.jog 0 @07:43:13.46 Archive.home.jog 0 @07:43:13.49 Toroid.home.jog 0 @07:43:13.52 PinPort.home.jog 0 @07:43:14.37 Plunge.in :homing @07:43:14.72 Plunge.jog 1488 @07:43:14.93 Lysis2.in :homing @07:43:14.98 Lysis1.in :homing @07:43:15.00 Archive.in :homing @07:43:15.02 Toroid.in :homing @07:43:15.04 PinPort.in :homing @07:43:15.08 Air.in :homing @07:43:15.29 Lysis2.jog 1621 @07:43:15.35 Lysis1.jog 277 @07:43:15.43 Archive.jog 304 @07:43:15.49 PinPort.jog 1241 @07:43:15.53 Air.jog 1431 @07:43:15.62 Toroid.jog 1365 @07:43:16.06 Plunge.setRawPosition! 50 @07:43:16.12 Plunge.seek :home @07:43:16.21 Lysis1.setRawPosition! 50 @07:43:16.27 Lysis1.seek :home @07:43:16.35 Archive.setRawPosition! 50 @07:43:16.49 Plunge.in :default @07:43:16.52 Archive.seek :home @07:43:16.68 Lysis1.in :default @07:43:16.82 Lysis2.setRawPosition! 50 @07:43:16.91 Archive.in :default @07:43:16.93 Lysis2.seek :home @07:43:17.09 Air.setRawPosition! 0 @07:43:17.14 Air.seek :home @07:43:17.24 Air.in :default @07:43:17.32 Lysis2.in :default @07:43:17.57 PinPort.setRawPosition! 3150 @07:43:17.63 PinPort.seek :home @07:43:20.36 Toroid.setRawPosition! 0 @07:43:20.41 Toroid.seek :home @07:43:20.49 Toroid.in :default @07:43:22.00 PinPort.in :default @07:43:22.34 Toroid.seek :clear @07:43:22.98 PinPort.in :unlocking @07:43:23.07 PinPort.seek :clear @07:43:26.49 Air.seek :clear @07:43:26.59 Lysis1.seek :clear @07:43:27.31 Lysis2.seek :clear @07:43:28.09 Archive.seek :clear @07:43:28.87 Carousel.in :default @07:43:28.99 Carousel.home.seek 12 @07:43:29.26 Selecting Cartridge 6 @07:43:29.56 Carousel.seek 6 @07:43:31.14 Carousel.reconfigure :align @07:43:31.27 Lysis1.in :fast @07:43:31.32 Lysis1.seek :locked @07:43:33.96 Plunge.seek :locked @07:43:34.13 Air.in :fast @07:43:34.18 Air.seek :locked @07:43:35.25 Carousel.reconfigure :locked @07:43:35.41 Powering cartridge bus @07:43:38.08 Gate.setIR 1750,5000,0,6 @07:43:38.20 Gate.setIR 0,5000,0,6 @07:43:38.27 PuckHeater.configure :default @07:43:38.33 Gate.power :heat,:ON,limit to 5000mA @07:43:38.49 Mounted lysis Cartridge board#J59943 @07:43:38.53 Using default cartridge heat loss curve for HeatingFunctionInCarousel @07:43:40.07 SPR.command "read_sequences" @07:43:40.62 SPR.command "stats_on" @07:43:40.77 SPR.command "start 0 1" @07:43:41.48 SPR.start /var/log/esp/spr26jul2907h.spr.bz2 @07:43:43.48 Waiting for baseline SPR stabilization @07:43:43.49 Handoff.select :waste @07:43:43.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @07:43:50.65 Syringe tare is 50% of full scale at 22C (drifted -0%) @07:43:50.67 PinPort.in :locking @07:43:50.72 PinPort.seek :locked @07:43:51.58 Archive.in :fast @07:43:51.67 Lysis2.in :fast @07:43:51.72 Lysis1.seek 1525uL @07:43:51.87 Archive.seek :empty @07:43:51.98 Lysis2.seek 1525uL @07:44:00.29 delayUntil "07:53:43.05" @07:53:43.06 Lysis2.in :DAmix @07:53:43.17 Lysis1.in :DAmix @07:53:43.78 Lysis2.seek 1275uL @07:53:43.87 Lysis1.seek 1275uL @07:54:18.86 delay 5 seconds @07:54:23.88 Handoff.select SPR @07:54:24.37 SPR.command "start 2 14" @07:54:25.70 SPR stabilized @07:54:25.89 delay 20 seconds @07:54:45.91 SPR.command "start 2 0" @07:54:48.53 Waiting up to 11:00 for SPR to enter state 1 @07:56:46.19 SPR.command "hold_on" @07:56:48.12 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:56:48.75 Lysis2.seek 975uL @07:56:48.82 Lysis1.seek 975uL @07:57:30.72 delay 15 seconds @07:57:45.75 SPR.command "hold_off" @07:57:49.18 Waiting up to 1:00:00 for SPR to enter state 7 @08:14:03.85 SPRsummary:4.38RIU,9.59RIU,4.22RIU,12.3RIU after 1924s @08:28:20.84 delay 20 seconds @08:28:40.86 SPR.command "start 2 0" @08:28:43.96 Waiting up to 11:00 for SPR to enter state 1 @08:30:41.64 SPR.command "hold_on" @08:30:43.02 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:30:43.76 Lysis2.seek 825uL @08:30:43.84 Lysis1.seek 825uL @08:31:04.91 delay 15 seconds @08:31:19.93 SPR.command "hold_off" @08:31:22.86 Waiting up to 1:00:00 for SPR to enter state 7 @09:01:55.33 SPR analysis completed /home/esp/esp2/lib/schedule.rb:557:in `initialize': Thread(0xb6976a68): deadlock (fatal) /home/esp/esp2/lib/schedule.rb:557:in `flush': Thread(0xb6976a68): deadlock (fatal) from /home/esp/esp2/lib/log.rb:536:in `append' from /home/esp/esp2/lib/rmutex.rb:92:in `synchronize' from /home/esp/esp2/lib/log.rb:517:in `append' from /home/esp/esp2/lib/log.rb:561:in `recordObject' from /home/esp/esp2/lib/log.rb:580:in `recordException' from /home/esp/esp2/lib/log.rb:733:in `recordException' from /home/esp/esp2/lib/rmutex.rb:99:in `synchronize' from /home/esp/esp2/lib/log.rb:732:in `recordException' from /home/esp/esp2/lib/log.rb:313:in `recordException' from /home/esp/esp2/lib/esp.rb:91:in `mainThread' from /home/esp/esp2/lib/esp.rb:371 @09:05:22.62EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @09:05:24.45 >BREAK< ESP3 Gateway v1.44 for core bus @09:05:24.47 >BREAK< ESP3 Gateway v1.44 for cartridge bus @09:05:28.30 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @09:05:29.01 Sea pressure (Abs) tare is 6% of full scale @09:05:31.56 Polling Can every 2:00 @09:05:31.60 Waiting for espclients... @09:05:32.86 Session Begun @09:05:49.55 -> ESP.shutdown @09:05:49.60 Gate.power :heat,:OFF @09:05:49.62 Gate.power :motor,:OFF @09:05:49.66 Gate.power :analytic1,:OFF @09:05:49.70 Gate.power :core,:OFF @09:05:49.74 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @09:06:26.51 -> slots {6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @09:11:31.28 -> Cart.unlock @09:11:31.36 Gate.power :sampler,:OFF,limit to 3500mA @09:11:31.38 Gate.power :skimmer,:OFF,limit to 3500mA @09:11:31.41 Gate.power :main,:ON,limit to 5000mA @09:11:31.46 Gate.power :analytic1,:ON,limit to 4000mA @09:11:31.65 Powering core bus @09:11:34.43 Plunge.configure :default @09:11:34.54 SamplePump.configure :default @09:11:34.64 Toroid.configure :default @09:11:34.76 Carousel.configure :default @09:11:34.87 Abs.configure :default @09:11:34.98 PhaseSeparator.configure :default @09:11:35.09 Archive.configure :default @09:11:35.19 Air.configure :default @09:11:35.28 Lysis1.configure :default @09:11:35.62 Lysis2.configure :default @09:11:35.72 PinPort.configure :default @09:11:35.82 All elf controllers running firmware version 4.94 @09:11:35.87 Gate.power :motor,:ON,limit to 5000mA @09:11:38.86 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @09:11:38.89 AnalyticModule::Unknown in preston -- SPR @09:11:39.31 Toroid.home.jog 0 @09:11:39.42 Plunge.home.jog 0 @09:11:40.13 Lysis2.home.jog 0 @09:11:40.18 Air.home.jog 0 @09:11:40.23 Carousel.home.jog 0 @09:11:40.26 Lysis1.home.jog 0 @09:11:40.32 Archive.home.jog 0 @09:11:40.36 PinPort.home.jog 0 @09:11:41.06 Plunge.in :homing @09:11:41.13 Toroid.in :homing @09:11:41.31 Plunge.jog 1482 @09:11:41.35 Toroid.jog 1366 @09:11:41.60 PinPort.in :homing @09:11:41.65 Lysis2.in :homing @09:11:41.70 Air.in :homing @09:11:41.75 Archive.in :homing @09:11:41.84 Lysis1.in :homing @09:11:42.07 PinPort.jog 3150 @09:11:42.13 Lysis2.jog 6397 @09:11:42.17 Air.jog 1247 @09:11:42.23 Lysis1.jog 6406 @09:11:42.32 Archive.jog 9650 @09:11:42.66 Plunge.setRawPosition! 50 @09:11:42.74 Plunge.seek :home @09:11:43.04 Plunge.in :default @09:11:43.54 Air.setRawPosition! 0 @09:11:43.61 Air.seek :home @09:11:43.72 Air.in :default @09:11:45.92 Lysis2.setRawPosition! 50 @09:11:46.01 Lysis2.seek :home @09:11:46.14 Lysis1.setRawPosition! 50 @09:11:46.16 Toroid.setRawPosition! 0 @09:11:46.29 Toroid.seek :home @09:11:46.34 Lysis1.seek :home @09:11:46.57 Toroid.in :default @09:11:46.59 Lysis2.in :default @09:11:46.76 PinPort.setRawPosition! 3150 @09:11:46.84 Lysis1.in :default @09:11:46.93 PinPort.seek :home @09:11:47.49 Archive.setRawPosition! 50 @09:11:47.58 Archive.seek :home @09:11:47.88 Archive.in :default @09:11:51.34 PinPort.in :default @09:11:51.70 Toroid.seek :clear @09:11:52.41 PinPort.in :unlocking @09:11:52.49 PinPort.seek :clear @09:11:55.90 Air.seek :clear @09:11:56.03 Lysis1.seek :clear @09:11:56.77 Lysis2.seek :clear @09:11:57.58 Archive.seek :clear @09:11:58.40 Carousel.in :default @09:11:58.54 Carousel.home.seek 6 @09:11:58.89 Carousel.in :default @09:11:59.03 Carousel.home.seek 6 @09:11:59.68 Plunge.in :unlocking @09:11:59.77 Plunge.seek :clear @09:12:34.88 -> SC.to 22 @09:12:34.93 Carousel.seek 22 Storage Carousel (SC) at 22 @09:13:11.84 -> slot 22=>:lyseHold_phyto {22 => { Type: :lyseHold_phyto, State: :dry}} @09:13:46.19 -> SC.to 14 @09:13:46.23 Carousel.seek 14 Storage Carousel (SC) at 14 @09:14:19.25 -> SC.to 52 @09:14:19.30 Carousel.seek 52 Storage Carousel (SC) at 52 @09:14:51.70 -> to 52 @09:14:52.06 Carousel.seek 52 @09:14:52.36 Carousel.reconfigure :align @09:14:52.50 Lysis1.in :fast @09:14:52.59 Lysis1.seek :locked @09:14:55.25 Plunge.in :default @09:14:55.37 Plunge.seek :locked @09:14:55.51 Air.in :fast @09:14:55.59 Air.seek :locked @09:14:56.69 Carousel.reconfigure :locked @09:14:56.93 Gate.setIR 1750,5000,0,6 @09:14:57.02 I2C::DuplicateAddress::Error in cartridge -- Multiple cartridge microcontrollers at address 0x09 @09:14:57.07 Gate.setIR 0,5000,0,6 @09:14:57.16 PuckHeater.configure :default @09:14:57.26 Gate.power :heat,:ON,limit to 5000mA @09:14:57.59 Mounted lysis Cartridge board#J59943 @09:14:57.64 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @09:15:04.40 -> slot 52=>:lyseHold_phyto {52 => { Type: :lyseHold_phyto, State: :missing}} @09:15:19.69 -> slot 52=>[:lyseHold_phyto,:dry] {52 => { Type: :lyseHold_phyto, State: :dry}} @09:15:21.19 -> to 22 @09:15:21.38 Gate.power :heat,:OFF @09:15:21.41 Gate.power :cartridge,:OFF @09:15:21.52 Carousel.in :default @09:15:21.67 Carousel.home.seek 52 @09:15:22.14 Air.in :unlocking @09:15:22.28 Air.seek :clear @09:15:23.88 Lysis1.in :unlocking @09:15:24.02 Lysis1.seek :clear @09:15:24.16 Plunge.in :unlocking @09:15:24.24 Plunge.seek :clear @09:15:25.39 Carousel.seek 22 @09:15:31.91 Carousel.reconfigure :align @09:15:32.06 Lysis1.in :fast @09:15:32.15 Lysis1.seek :locked @09:15:32.32 Slide::Warning in core -- Storage Carousel (SC) reseek at 22 + 3 counts (sensed 22 + 4 counts) @09:15:34.80 Plunge.in :default @09:15:34.89 Plunge.seek :locked @09:15:35.06 Air.in :fast @09:15:35.14 Air.seek :locked @09:15:36.24 Carousel.reconfigure :locked @09:15:36.41 Powering cartridge bus @09:15:39.08 Gate.setIR 1750,5000,0,6 @09:15:39.23 Gate.setIR 0,5000,0,6 @09:15:39.31 PuckHeater.configure :default @09:15:39.40 Gate.power :heat,:ON,limit to 5000mA @09:15:39.60 Mounted lysis Cartridge board#J60003 @09:15:39.65 Using default cartridge heat loss curve for HeatingFunctionInCarousel :lyseHold_phyto @09:15:45.41 -> Cart.unlock @09:15:45.47 Gate.power :heat,:OFF @09:15:45.49 Gate.power :cartridge,:OFF @09:15:45.60 Carousel.in :default @09:15:45.76 Carousel.home.seek 22 @09:15:46.22 Air.in :unlocking @09:15:46.39 Air.seek :clear @09:15:47.48 Lysis1.in :unlocking @09:15:47.63 Lysis1.seek :clear @09:15:47.76 Plunge.in :unlocking @09:15:47.88 Plunge.seek :clear @09:20:03.80 -> Cart.dry [52,22] @09:20:06.44 -> exit @09:20:06.46 Session Ended @11:00:56.40EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @11:01:00.13 >BREAK< ESP3 Gateway v1.44 for core bus @11:01:00.16 >BREAK< ESP3 Gateway v1.44 for cartridge bus @11:01:03.95 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @11:01:04.68 Sea pressure (Abs) tare is 6% of full scale @11:01:07.16 Polling Can every 2:00 @11:01:07.22 Waiting for espclients... @11:01:08.21 Session Begun @11:01:15.13 -> ESP.ready! @11:01:15.19 Gate.power :sampler,:OFF,limit to 3500mA @11:01:15.21 Gate.power :skimmer,:OFF,limit to 3500mA @11:01:15.25 Gate.power :main,:ON,limit to 5000mA @11:01:15.29 Gate.power :analytic1,:ON,limit to 4000mA @11:01:15.86 Powering core bus @11:01:18.63 Plunge.configure :default @11:01:18.74 SamplePump.configure :default @11:01:18.84 Toroid.configure :default @11:01:18.96 Carousel.configure :default @11:01:19.06 Abs.configure :default @11:01:19.43 PhaseSeparator.configure :default @11:01:19.54 Archive.configure :default @11:01:19.64 Air.configure :default @11:01:19.73 Lysis1.configure :default @11:01:19.82 Lysis2.configure :default @11:01:19.92 PinPort.configure :default @11:01:19.99 All elf controllers running firmware version 4.94 @11:01:20.02 Gate.power :motor,:ON,limit to 5000mA @11:01:22.65 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @11:01:22.68 AnalyticModule::Unknown in nadia -- SPR @11:01:23.07 Plunge.home.jog 0 @11:01:23.88 PinPort.home.jog 0 @11:01:23.91 Lysis1.home.jog 0 @11:01:23.94 Carousel.home.jog 0 @11:01:24.00 Air.home.jog 0 @11:01:24.04 Archive.home.jog 0 @11:01:24.07 Lysis2.home.jog 0 @11:01:24.10 Toroid.home.jog 0 @11:01:24.93 Plunge.in :homing @11:01:25.22 Plunge.jog 584 @11:01:25.30 Lysis2.in :homing @11:01:25.35 Air.in :homing @11:01:25.40 Toroid.in :homing @11:01:25.44 PinPort.in :homing @11:01:25.48 Lysis1.in :homing @11:01:25.52 Archive.in :homing @11:01:25.86 PinPort.jog 1307 @11:01:25.93 Lysis2.jog 1620 @11:01:25.97 Lysis1.jog 1600 @11:01:26.01 Air.jog 1431 @11:01:26.05 Toroid.jog 1351 @11:01:26.12 Archive.jog 1591 @11:01:26.56 Plunge.setRawPosition! 50 @11:01:26.63 Plunge.seek :home @11:01:26.92 Plunge.in :default @11:01:27.44 Air.setRawPosition! 0 @11:01:27.52 Air.seek :home @11:01:27.68 Lysis2.setRawPosition! 50 @11:01:27.73 Lysis1.setRawPosition! 50 @11:01:27.85 Lysis2.seek :home @11:01:27.89 Lysis1.seek :home @11:01:28.12 Air.in :default @11:01:28.15 Archive.setRawPosition! 50 @11:01:28.19 PinPort.setRawPosition! 3150 @11:01:28.41 PinPort.seek :home @11:01:28.47 Archive.seek :home @11:01:28.69 Lysis1.in :default @11:01:28.71 Lysis2.in :default @11:01:28.90 Archive.in :default @11:01:30.86 Toroid.setRawPosition! 0 @11:01:30.93 Toroid.seek :home @11:01:31.03 Toroid.in :default @11:01:32.92 PinPort.in :default @11:01:33.27 Toroid.seek :clear @11:01:33.44 PinPort.in :unlocking @11:01:33.53 PinPort.seek :clear @11:01:36.89 Air.seek :clear @11:01:37.01 Lysis1.seek :clear @11:01:37.76 Lysis2.seek :clear @11:01:38.58 Archive.seek :clear @11:01:39.38 Carousel.in :default @11:01:39.52 Carousel.home.seek 26 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 26 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.14psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:01:54.14 -> ESP.reready! @11:01:54.19 Plunge.in :default @11:01:54.26 Toroid.in :default @11:01:54.49 Carousel.in :default @11:01:54.58 Archive.in :default @11:01:54.67 Air.in :default @11:01:54.71 Lysis1.in :default @11:01:54.79 Lysis2.in :default @11:01:54.81 Plunge.home.jog 0 @11:01:54.90 PinPort.in :default @11:01:55.02 Toroid.home.jog 0 @11:01:55.23 Carousel.home.jog 0 @11:01:55.41 Archive.home.jog 0 @11:01:55.47 Air.home.jog 0 @11:01:55.55 Lysis2.home.jog 0 @11:01:55.61 PinPort.home.jog 0 @11:01:55.72 Lysis1.home.jog 0 @11:01:56.68 Toroid.in :homing @11:01:56.78 Plunge.in :homing @11:01:57.00 Toroid.jog 1355 @11:01:57.02 Plunge.jog 434 @11:01:57.30 Lysis2.in :homing @11:01:57.36 Lysis1.in :homing @11:01:57.40 Archive.in :homing @11:01:57.45 PinPort.in :homing @11:01:57.50 Air.in :homing @11:01:57.67 Lysis2.jog 1621 @11:01:57.79 PinPort.jog 1216 @11:01:57.83 Archive.jog 1598 @11:01:57.88 Lysis1.jog 1598 @11:01:57.96 Air.jog 1424 @11:01:58.31 Plunge.setRawPosition! 50 @11:01:58.42 Plunge.seek :home @11:01:58.72 Plunge.in :default @11:01:59.24 Lysis2.setRawPosition! 50 @11:01:59.32 Lysis2.seek :home @11:01:59.43 Air.setRawPosition! 0 @11:01:59.49 Archive.setRawPosition! 50 @11:01:59.63 Archive.seek :home @11:01:59.67 Air.seek :home @11:01:59.92 Lysis2.in :default @11:01:59.96 Lysis1.setRawPosition! 50 @11:02:00.00 PinPort.setRawPosition! 3150 @11:02:00.04 Air.in :default @11:02:00.26 Lysis1.seek :home @11:02:00.29 PinPort.seek :home @11:02:00.54 Archive.in :default @11:02:00.69 Lysis1.in :default @11:02:01.96 Toroid.setRawPosition! 0 @11:02:02.04 Toroid.seek :home @11:02:02.14 Toroid.in :default @11:02:04.83 PinPort.in :default @11:02:05.20 Toroid.seek :clear @11:02:05.38 PinPort.in :unlocking @11:02:05.46 PinPort.seek :clear @11:02:08.89 Air.seek :clear @11:02:09.01 Lysis1.seek :clear @11:02:09.74 Lysis2.seek :clear @11:02:10.56 Archive.seek :clear @11:02:11.39 Carousel.in :default @11:02:11.54 Carousel.home.seek 26 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 26 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @0.13psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:02:16.35 -> Cart.dry [52,22] @11:02:41.50 -> lysis=:lyseHold_phyto :lyseHold_phyto @11:02:43.79 -> lysys :lysys @11:02:46.43 -> lysis :lyseHold_phyto @11:04:28.92 -> Power.sampler :ON @11:04:28.94 Gate.power :sampler,:ON true @11:07:32.90 -> 3.times{Sampler.refill};BACsampler.prime @11:07:32.92 Bypass.open @11:07:34.52 Intake.open @11:07:35.99 SamplePump.setPosition! 0ml @11:07:36.13 SamplePump.seek 30ml @11:08:16.08 Bypass.close @11:08:17.60 Exhaust.open @11:08:19.23 SamplePump.seek 55ml @11:08:52.64 delay 1 second @11:08:53.67 Intake.close @11:08:53.71 Exhaust.close @11:08:55.33 Bypass.open @11:08:56.84 Intake.open @11:08:58.28 SamplePump.setPosition! 0ml @11:08:58.45 SamplePump.seek 30ml @11:09:38.41 Bypass.close @11:09:39.96 Exhaust.open @11:09:41.52 SamplePump.seek 55ml @11:10:14.92 delay 1 second @11:10:15.95 Intake.close @11:10:16.03 Exhaust.close @11:10:17.60 Bypass.open @11:10:19.18 Intake.open @11:10:20.65 SamplePump.setPosition! 0ml @11:10:20.79 SamplePump.seek 30ml @11:11:00.74 Bypass.close @11:11:02.29 Exhaust.open @11:11:03.92 SamplePump.seek 55ml @11:11:37.31 delay 1 second @11:11:38.35 Intake.close @11:11:38.46 Exhaust.close @11:11:40.18 Priming sample loop w/200ml, bypass w/10ml @11:11:40.21 SamplePump.setPosition! 0ml @11:11:40.73 Bypass.open @11:11:43.11 Intake.open @11:11:44.78 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:11:47.40 Vacuum tare is 53% of full scale at 24C (drifted 2%) @11:11:47.58 Abs.in :default @11:11:47.65 Abs.hold @11:11:47.75 PhaseSeparator.in :full @11:11:47.83 PhaseSeparator.hold @11:11:48.11 SamplePump.in :start @11:11:48.20 SamplePump.seek 10ml,1:10 @11:11:48.80 SamplePump.configure :prime @11:12:01.76 Abs.stop @11:12:01.83 PhaseSeparator.stop @11:12:01.91 SamplePump.setPosition! 0ml @11:12:01.99 Exhaust.open @11:12:04.18 Bypass.close @11:12:06.61 Abs.in :default @11:12:06.70 Abs.hold @11:12:06.81 PhaseSeparator.hold @11:12:07.16 SamplePump.in :start @11:12:07.25 SamplePump.seek 200ml,13:50 @11:12:07.85 SamplePump.configure :prime @11:16:30.07 Abs.stop @11:16:30.14 PhaseSeparator.stop BACsampler @11:18:13.47 -> Power.sampler :OFF @11:18:13.49 Gate.power :sampler,:OFF false @11:18:30.67 -> lysis :lyseHold_phyto @11:20:02.36 -> SC Storage Carousel (SC) at 26 @11:20:08.75 -> SC.to 60 @11:20:08.80 Carousel.seek 60 Storage Carousel (SC) at 60 @11:20:19.22 -> SC.to 1 @11:20:19.26 Carousel.seek 1 Storage Carousel (SC) at 1 @11:21:30.26 -> Cartridge.lock @11:21:30.41 Carousel.reconfigure :align @11:21:30.56 Lysis1.in :fast @11:21:30.64 Lysis1.seek :locked @11:21:33.29 Plunge.seek :locked @11:21:33.50 Air.in :fast @11:21:33.58 Air.seek :locked @11:21:34.67 Carousel.reconfigure :locked @11:21:34.84 Powering cartridge bus @11:21:37.51 Gate.setIR 1750,5000,0,6 @11:21:37.64 Gate.setIR 0,5000,0,6 @11:21:37.75 Gate.power :cartridge,:OFF @11:21:37.79 Mounted archive Cartridge :archiveHiBiomass_bac @11:22:26.50 -> Cart.unlock @11:22:26.61 Carousel.in :default @11:22:26.75 Carousel.home.seek 1 @11:22:27.58 Air.in :unlocking @11:22:27.74 Air.seek :clear @11:22:28.82 Lysis1.in :unlocking @11:22:29.00 Lysis1.seek :clear @11:22:29.09 Plunge.in :unlocking @11:22:29.20 Plunge.seek :clear @11:22:34.02 -> Cart.lock @11:22:34.14 Carousel.reconfigure :align @11:22:34.27 Lysis1.in :fast @11:22:34.39 Lysis1.seek :locked @11:22:37.04 Plunge.in :default @11:22:37.12 Plunge.seek :locked @11:22:37.31 Air.in :fast @11:22:37.39 Air.seek :locked @11:22:38.49 Carousel.reconfigure :locked @11:22:38.67 Powering cartridge bus @11:22:41.33 Gate.setIR 1750,5000,0,6 @11:22:41.46 Gate.setIR 0,5000,0,6 @11:22:41.57 Gate.power :cartridge,:OFF @11:22:41.62 Mounted archive Cartridge :archiveHiBiomass_bac @11:23:11.86 -> Cart.unlock @11:23:11.97 Carousel.in :default @11:23:12.11 Carousel.home.seek 1 @11:23:12.60 Air.in :unlocking @11:23:12.77 Air.seek :clear @11:23:13.87 Lysis1.in :unlocking @11:23:14.07 Lysis1.seek :clear @11:23:14.17 Plunge.in :unlocking @11:23:14.25 Plunge.seek :clear @11:23:16.99 -> Cart.dry [52,22] @11:23:37.71 -> fire lysis @11:23:38.60 Toroid.seek :clear @11:23:38.84 Air.in :default @11:23:38.92 Air.seek :clear @11:23:39.03 Lysis1.in :default @11:23:39.10 Lysis1.seek :clear @11:23:39.22 Lysis2.seek :clear @11:23:39.34 Archive.seek :clear @11:23:39.45 Carousel.in :default @11:23:39.60 Carousel.home.seek 1 @11:23:39.91 Selecting Cartridge 52 @11:23:40.69 Carousel.seek 52 @11:23:51.45 Carousel.reconfigure :align @11:23:51.60 Lysis1.in :fast @11:23:51.77 Lysis1.seek :locked @11:23:51.89 Slide::Warning in core -- Storage Carousel (SC) reseek at 52 - 4 counts @11:23:54.46 Plunge.in :default @11:23:54.56 Plunge.seek :locked @11:23:54.73 Air.in :fast @11:23:54.80 Air.seek :locked @11:23:55.91 Carousel.reconfigure :locked @11:23:56.10 Powering cartridge bus @11:23:58.78 Gate.setIR 1750,5000,0,6 @11:23:58.91 Gate.setIR 0,5000,0,6 @11:23:59.01 PuckHeater.configure :default @11:23:59.10 Gate.power :heat,:ON,limit to 5000mA @11:23:59.36 Mounted lysis Cartridge board#J59981 @11:23:59.42 Using default cartridge heat loss curve for HeatingFunctionInCarousel @11:23:59.76 Preparing to filter lyseHold_phyto cartridge in slot 52 @11:24:00.04 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:24:06.65 Intake Head Pressure tare is 0% of full scale at 25C (drifted 1%) @11:24:06.67 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:24:06.73 PhaseSeparator.in :filling @11:24:06.80 PhaseSeparator.hold @11:24:06.90 Gate.power :sampler,:ON @11:24:14.03 Head pressure reached 3.37psi in 8 seconds @11:24:14.06 Air.in :default @11:24:14.25 Air.seek :sealed @11:24:14.28 Priming sample loop w/20ml @11:24:14.39 SamplePump.setPosition! 0ml @11:24:14.50 Exhaust.close @11:24:16.02 Bypass.open @11:24:18.47 Exhaust.open @11:24:20.65 Bypass.close @11:24:23.02 Abs.in :default @11:24:23.10 Abs.hold @11:24:23.19 PhaseSeparator.in :full @11:24:23.27 PhaseSeparator.hold @11:24:24.10 Waiting for sampler to finish priming... @11:24:24.12 delayUntil "11:25:06.92" @11:25:06.99 SamplePump.in :start @11:25:07.13 SamplePump.seek 20ml,1:50 @11:25:07.68 SamplePump.configure :prime @11:25:33.79 Abs.stop @11:25:33.87 PhaseSeparator.stop @11:25:33.96 Bypass.open @11:25:35.51 Intake.close @11:25:37.12 Toroid.in :hold @11:25:37.20 Toroid.seek :crack @11:25:39.69 Toroid.seek :cartridge @11:25:41.83 Toroid.in :default @11:25:41.91 Toroid.seek :clear @11:25:45.92 Pressurizing cartridge to 14psi above 15.3psia @11:25:45.94 SamplePump.setPosition! 0ml @11:25:46.03 Intake.open @11:25:47.52 Bypass.close @11:25:49.08 Exhaust.close @11:25:50.71 Abs.in :default @11:25:50.80 Abs.hold @11:25:50.90 PhaseSeparator.hold @11:25:51.18 SamplePump.in :charge @11:25:51.26 SamplePump.seek 2.3ml,38 seconds @11:26:03.42 Abs.stop @11:26:03.50 PhaseSeparator.stop @11:26:03.59 Intake.close @11:26:05.16 Pressurized cartridge with 0.609ml to 83.8psi @11:26:05.17 Isolated @24.1psia (8.9psi) @11:26:05.24 Verifying cartridge holds at least 0.65..0.85 of 24.1psia @11:26:05.29 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:26:09.91 Cartridge pressure fell 1% to 23.8psia @11:26:10.02 Sampling 1000.0ml @11:26:10.05 SamplePump.setPosition! 0ml @11:26:10.13 Exhaust.open @11:26:11.64 Intake.open @11:26:13.30 Abs.in :default @11:26:13.39 Abs.hold @11:26:13.49 PhaseSeparator.hold @11:26:14.42 SamplePump.in :start @11:26:14.52 SamplePump.seek 1000ml,56:04 @11:26:15.11 SamplePump.configure :sample @11:26:17.51 Cartridge 52 filtering 2/1000ml at 0.70ml/s with 11.4psi vacuum @11:27:17.56 Cartridge 52 filtering 45/1000ml at 0.73ml/s with 12.7psi vacuum @11:28:17.57 Cartridge 52 filtering 89/1000ml at 0.73ml/s with 13.7psi vacuum @11:29:17.56 Cartridge 52 filtering 132/1000ml at 0.72ml/s with 11.8psi vacuum @11:30:17.58 Cartridge 52 filtering 176/1000ml at 0.72ml/s with 12.9psi vacuum @11:31:17.57 Cartridge 52 filtering 219/1000ml at 0.72ml/s with 14.0psi vacuum @11:32:00.54 Session Begun @11:32:17.56 Cartridge 52 filtering 262/1000ml at 0.72ml/s with 13.2psi vacuum @11:33:17.57 Cartridge 52 filtering 304/1000ml at 0.71ml/s with 14.4psi vacuum @11:33:24.98 -> exit @11:33:25.01 Session Ended @11:33:32.84 Session Begun @11:33:40.28 -> hi :hi @11:34:17.57 Cartridge 52 filtering 347/1000ml at 0.71ml/s with 14.7psi vacuum @11:35:17.57 Cartridge 52 filtering 390/1000ml at 0.71ml/s with 14.8psi vacuum @11:36:17.56 Cartridge 52 filtering 433/1000ml at 0.71ml/s with 11.0psi vacuum @11:37:17.56 Cartridge 52 filtering 476/1000ml at 0.72ml/s with 11.2psi vacuum @11:38:17.57 Cartridge 52 filtering 519/1000ml at 0.73ml/s with 11.1psi vacuum @11:39:17.57 Cartridge 52 filtering 563/1000ml at 0.73ml/s with 6.6psi vacuum 5.6m deep (22.9psia) @11:40:17.57 Cartridge 52 filtering 606/1000ml at 0.72ml/s with 10.5psi vacuum 4.1m deep (20.7psia) @11:41:17.56 Cartridge 52 filtering 650/1000ml at 0.72ml/s with 8.0psi vacuum @11:42:17.58 Cartridge 52 filtering 693/1000ml at 0.72ml/s with 10.1psi vacuum @11:43:17.56 Cartridge 52 filtering 736/1000ml at 0.72ml/s with 8.3psi vacuum 4.0m deep (20.6psia) @11:44:17.57 Cartridge 52 filtering 779/1000ml at 0.72ml/s with 11.5psi vacuum @11:45:17.57 Cartridge 52 filtering 822/1000ml at 0.71ml/s with 10.8psi vacuum @11:46:17.57 Cartridge 52 filtering 864/1000ml at 0.71ml/s with 8.3psi vacuum @11:47:17.56 Cartridge 52 filtering 907/1000ml at 0.71ml/s with 10.7psi vacuum @11:48:17.56 Cartridge 52 filtering 949/1000ml at 0.70ml/s with 13.4psi vacuum @11:49:17.56 Cartridge 52 filtering 991/1000ml at 0.70ml/s with 10.1psi vacuum @11:49:30.16 Abs.stop @11:49:30.25 PhaseSeparator.stop @11:49:30.41 Intake.close @11:49:30.48 Exhaust.close @11:49:30.79 Toroid.in :hold @11:49:30.89 Toroid.seek :bypass @11:49:34.54 Toroid.in :default @11:49:34.65 Toroid.seek :clear @11:49:38.26 Sampled 1000.0ml @11:49:39.45 Handoff.select :waste @11:49:39.98 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:49:41.50 Gate.power :sampler,:OFF @11:49:54.10 Syringe tare is 50% of full scale at 26C (drifted -1%) @11:49:54.14 PinPort.in :locking @11:49:54.25 PinPort.seek :locked @11:49:55.12 Archive.in :fast @11:49:55.23 Lysis2.in :fast @11:49:55.30 Lysis1.seek 1250uL @11:49:55.36 Archive.seek :empty @11:49:55.59 Lysis2.seek :empty @11:50:04.09 Evacuating puck with filter bubble point of 20psi @11:50:04.13 PinPort.seek :locked @11:50:04.27 Handoff.select :waste @11:50:04.32 Plunge.seek :puckBottom @11:50:05.72 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:50:17.83 Air syringe pumping up to 1 strokes to reach 4psi @11:50:17.84 Air stroke #1 of 1 @11:50:18.00 Air.in :pressurize @11:50:18.07 Air.seek :pressurized /home/esp/esp2/lib/thread.rb:989: [BUG] gc_sweep(): unknown data type 0x0(0xb686de20) [1/30] ruby 1.8.9 (2026-04-22 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] @11:53:01.49EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @11:53:03.25 >BREAK< ESP3 Gateway v1.44 for core bus @11:53:03.28 >BREAK< ESP3 Gateway v1.44 for cartridge bus @11:53:06.98 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @11:53:07.66 Sea pressure (Abs) tare is 6% of full scale @11:53:10.14 Polling Can every 2:00 @11:53:10.18 Waiting for espclients... @11:53:27.48 Session Begun @11:53:44.87 -> slots {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 22 => { Type: :lyseHold_phyto, State: :dry}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 52 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @11:53:59.11 -> Cart.dry [22] @11:54:03.31 -> SC Storage Carousel (SC) at 52 @11:54:33.71 -> slot 52=>[:dry] {52 => { Type: :unknown, State: :dry}} @11:54:41.54 -> Cart.dry [52,22] @11:55:31.71 -> BACsampler.goalVolume 1000 @11:55:48.91 -> BACsampler.goalVolume=100 100 @11:56:42.51 -> lysis=:lyseHold_phyto :lyseHold_phyto @11:57:30.03 -> start {fire lysis} # @11:57:30.40 Gate.power :sampler,:OFF,limit to 3500mA @11:57:30.43 Gate.power :skimmer,:OFF,limit to 3500mA @11:57:30.47 Gate.power :analytic1,:ON,limit to 4000mA @11:57:31.17 Plunge.configure :default @11:57:31.26 Plunge.hold @11:57:31.35 Plunge.configure :default @11:57:31.52 SamplePump.configure :default @11:57:31.70 Toroid.configure :default @11:57:31.79 Toroid.hold @11:57:31.88 Toroid.configure :default @11:57:32.06 Carousel.configure :default @11:57:32.14 Carousel.hold @11:57:32.24 Carousel.configure :default @11:57:32.46 Abs.configure :default @11:57:32.89 PhaseSeparator.configure :default @11:57:33.06 Archive.configure :default @11:57:33.14 Archive.hold @11:57:33.24 Archive.configure :default @11:57:33.41 Air.configure :default @11:57:33.48 Air.hold @11:57:33.58 Air.configure :default @11:57:33.72 Lysis1.configure :default @11:57:33.80 Lysis1.hold @11:57:33.90 Lysis1.configure :default @11:57:34.05 Lysis2.configure :default @11:57:34.13 Lysis2.hold @11:57:34.22 Lysis2.configure :default @11:57:34.66 PinPort.configure :default @11:57:34.74 PinPort.hold @11:57:34.85 PinPort.configure :default @11:57:34.93 All elf controllers running firmware version 4.94 @11:57:36.00 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @11:57:36.04 AnalyticModule::Unknown in detached from andia -- SPR @11:57:36.82 Toroid.seek :clear @11:57:37.03 Air.seek :clear @11:57:41.80 Lysis1.seek :clear @11:57:49.58 Lysis2.seek :clear @11:58:08.18 Archive.seek :clear @11:58:26.79 Carousel.in :default @11:58:26.95 Carousel.home.seek 52 @11:58:27.31 Selecting Cartridge 22 @11:58:27.44 Gate.power :heat,:OFF @11:58:27.47 Gate.power :cartridge,:OFF @11:58:27.59 PinPort.in :unlocking @11:58:27.68 PinPort.seek :clear @11:58:28.54 Carousel.in :default @11:58:29.16 Carousel.home.seek 52 @11:58:29.82 Plunge.in :unlocking @11:58:29.91 Plunge.seek :clear @11:58:31.77 Carousel.seek 22 @11:58:38.27 Carousel.reconfigure :align @11:58:38.46 Lysis1.in :fast @11:58:38.54 Lysis1.seek :locked @11:58:41.19 Plunge.in :default @11:58:41.29 Plunge.seek :locked @11:58:41.48 Air.in :fast @11:58:41.57 Air.seek :locked @11:58:42.67 Carousel.reconfigure :locked @11:58:42.85 Powering cartridge bus @11:58:46.50 Gate.setIR 1750,5000,0,6 @11:58:46.63 Gate.setIR 0,5000,0,6 @11:58:46.72 PuckHeater.configure :default @11:58:46.82 Gate.power :heat,:ON,limit to 5000mA @11:58:47.08 Mounted lysis Cartridge board#J60003 @11:58:47.13 Using default cartridge heat loss curve for HeatingFunctionInCarousel @11:58:47.47 Preparing to filter lyseHold_phyto cartridge in slot 22 @11:58:47.75 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:58:54.42 Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) @11:58:54.45 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:58:54.51 PhaseSeparator.in :filling @11:58:54.57 PhaseSeparator.hold @11:58:54.67 Gate.power :sampler,:ON @11:59:01.73 Head pressure reached 3.31psi in 8 seconds @11:59:01.77 Air.in :default @11:59:01.92 Air.seek :sealed @11:59:02.00 Priming sample loop w/200ml, bypass w/10ml @11:59:02.04 SamplePump.setPosition! 0ml @11:59:02.15 Exhaust.close @11:59:02.28 Bypass.open @11:59:04.60 Intake.open @11:59:06.14 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:59:09.30 Vacuum tare is 53% of full scale at 26C (drifted 2%) @11:59:09.49 Abs.in :default @11:59:09.57 Abs.hold @11:59:09.68 PhaseSeparator.in :full @11:59:09.77 PhaseSeparator.hold @11:59:10.03 Waiting for sampler to finish priming... @11:59:10.05 delayUntil "11:59:54.69" @11:59:54.76 SamplePump.in :start @11:59:54.90 SamplePump.seek 10ml,1:10 @11:59:55.45 SamplePump.configure :prime @12:00:08.48 Abs.stop @12:00:08.56 PhaseSeparator.stop @12:00:08.64 SamplePump.setPosition! 0ml @12:00:08.71 Exhaust.open @12:00:10.91 Bypass.close @12:00:13.33 Abs.in :default @12:00:13.42 Abs.hold @12:00:13.54 PhaseSeparator.hold @12:00:13.90 SamplePump.in :start @12:00:14.00 SamplePump.seek 200ml,13:50 @12:00:14.59 SamplePump.configure :prime @12:04:36.88 Abs.stop @12:04:36.96 PhaseSeparator.stop @12:04:37.09 Bypass.open @12:04:38.66 Intake.close @12:04:40.27 Toroid.in :hold @12:04:40.37 Toroid.seek :crack @12:04:42.90 Toroid.seek :cartridge @12:04:45.04 Toroid.in :default @12:04:45.13 Toroid.seek :clear @12:04:49.13 Pressurizing cartridge to 14psi above 15.5psia @12:04:49.15 SamplePump.setPosition! 0ml @12:04:49.24 Intake.open @12:04:50.72 Bypass.close @12:04:52.25 Exhaust.close @12:04:53.98 Abs.in :default @12:04:54.06 Abs.hold @12:04:54.16 PhaseSeparator.hold @12:04:54.46 SamplePump.in :charge @12:04:54.56 SamplePump.seek 2.3ml,38 seconds @12:05:08.02 Abs.stop @12:05:08.13 PhaseSeparator.stop @12:05:08.21 Intake.close @12:05:09.75 Pressurized cartridge with 0.690ml to 14.1psi @12:05:09.77 Isolated @28.4psia (12.9psi) @12:05:09.82 Verifying cartridge holds at least 0.65..0.85 of 28.4psia @12:05:09.89 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:05:14.51 Cartridge pressure fell 1% to 28.0psia @12:05:14.64 Sampling 1000.0ml @12:05:14.66 SamplePump.setPosition! 0ml @12:05:14.74 Exhaust.open @12:05:16.36 Intake.open @12:05:18.03 Abs.in :default @12:05:18.11 Abs.hold @12:05:18.22 PhaseSeparator.hold @12:05:18.63 SamplePump.in :start @12:05:18.72 SamplePump.seek 1000ml,56:04 @12:05:19.32 SamplePump.configure :sample @12:05:21.73 Cartridge 22 filtering 2/1000ml at 0.63ml/s with 10.8psi vacuum @12:06:21.78 Cartridge 22 filtering 43/1000ml at 0.69ml/s with 7.6psi vacuum 4.2m deep (20.8psia) @12:07:14.58 -> PhytoSampler.goalVolume 1000 @12:07:18.82 -> PhytoSampler.goalVolume=100 100 @12:07:21.77 Cartridge 22 filtering 85/1000ml at 0.69ml/s with 7.4psi vacuum 5.0m deep (22.1psia) @12:07:47.70 -> slots 52=>nil {52 => nil} @12:07:58.26 -> slot 52=>:dry {52 => { Type: :unknown, State: :dry}} @12:08:21.78 Cartridge 22 filtering 126/1000ml at 0.68ml/s with 11.4psi vacuum @12:09:21.78 Cartridge 22 filtering 166/1000ml at 0.68ml/s with 11.1psi vacuum @12:10:21.78 Cartridge 22 filtering 207/1000ml at 0.67ml/s with 11.4psi vacuum @12:11:21.79 Cartridge 22 filtering 247/1000ml at 0.66ml/s with 11.1psi vacuum @12:12:21.77 Cartridge 22 filtering 286/1000ml at 0.66ml/s with 9.0psi vacuum @12:13:21.78 Cartridge 22 filtering 325/1000ml at 0.66ml/s with 11.1psi vacuum @12:14:21.78 Cartridge 22 filtering 364/1000ml at 0.65ml/s with 7.8psi vacuum 4.4m deep (21.1psia) @12:15:21.78 Cartridge 22 filtering 402/1000ml at 0.64ml/s with 9.8psi vacuum @12:16:21.78 Cartridge 22 filtering 441/1000ml at 0.64ml/s with 9.7psi vacuum @12:17:21.78 Cartridge 22 filtering 478/1000ml at 0.63ml/s with 9.5psi vacuum @12:18:21.77 Cartridge 22 filtering 516/1000ml at 0.63ml/s with 11.6psi vacuum @12:19:21.77 Cartridge 22 filtering 553/1000ml at 0.62ml/s with 11.8psi vacuum @12:20:21.78 Cartridge 22 filtering 591/1000ml at 0.62ml/s with 9.3psi vacuum @12:21:21.78 Cartridge 22 filtering 627/1000ml at 0.61ml/s with 13.3psi vacuum @12:22:22.32 Cartridge 22 filtering 664/1000ml at 0.61ml/s with 11.6psi vacuum @12:23:21.78 Cartridge 22 filtering 701/1000ml at 0.61ml/s with 12.8psi vacuum @12:24:21.79 Cartridge 22 filtering 737/1000ml at 0.61ml/s with 11.8psi vacuum @12:25:21.78 Cartridge 22 filtering 774/1000ml at 0.61ml/s with 12.2psi vacuum @12:26:21.78 Cartridge 22 filtering 811/1000ml at 0.62ml/s with 9.1psi vacuum @12:27:21.78 Cartridge 22 filtering 847/1000ml at 0.60ml/s with 16.1psi vacuum @12:28:12.38 Session Ended @12:28:21.78 Cartridge 22 filtering 883/1000ml at 0.60ml/s with 18.0psi vacuum @12:29:21.79 Cartridge 22 filtering 919/1000ml at 0.60ml/s with 9.4psi vacuum @12:30:21.77 Cartridge 22 filtering 955/1000ml at 0.60ml/s with 8.4psi vacuum @12:31:21.78 Cartridge 22 filtering 991/1000ml at 0.60ml/s with 7.4psi vacuum @12:31:36.24 Abs.stop @12:31:36.29 PhaseSeparator.stop @12:31:36.40 Intake.close @12:31:36.49 Exhaust.close @12:31:36.70 Toroid.in :hold @12:31:36.76 Toroid.seek :bypass @12:31:40.35 Toroid.in :default @12:31:40.44 Toroid.seek :clear @12:31:44.01 Sampled 1000.0ml @12:31:44.56 Handoff.select :waste @12:31:45.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:31:47.16 Gate.power :sampler,:OFF @12:31:51.67 Syringe tare is 50% of full scale at 26C (drifted -1%) @12:31:51.70 PinPort.in :locking @12:31:51.75 PinPort.seek :locked @12:31:52.58 Archive.in :fast @12:31:52.68 Lysis2.in :fast @12:31:52.73 Lysis1.seek 1250uL @12:31:52.77 Archive.seek :empty @12:31:52.89 Lysis2.seek :empty @12:32:01.40 Evacuating puck with filter bubble point of 20psi @12:32:01.42 PinPort.seek :locked @12:32:01.51 Handoff.select :waste @12:32:01.53 Plunge.seek :puckBottom @12:32:02.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:32:10.04 Air syringe pumping up to 1 strokes to reach 4psi @12:32:10.05 Air stroke #1 of 1 @12:32:10.13 Air.in :pressurize @12:32:10.18 Air.seek :pressurized @12:32:15.43 1.0psi after pressurize @12:32:15.45 Air.in :compress @12:32:15.50 Air.seek :evac @12:32:18.22 Reached 4.5 of 4.0psi after pumping 1551uL (0.87 strokes) @12:32:18.23 Air.in :default @12:32:18.28 Air.hold @12:32:18.40 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:32:46.48 Stabilized at 13.6psi after evac to waste @12:32:46.49 Handoff.select :blocked @12:32:47.48 Handoff.select :waste @12:32:48.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:33:14.65 Handoff.select :blocked @12:33:15.14 Plunge.seek :puckTop @12:33:16.01 Overpressure in PROCESSING -- Waste port clogged (14.9psi) @12:33:16.03 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" @12:33:57.19 Session Begun @12:34:12.89 -> showlog @12:34:41.41 -> Syringe.psi 15.8342346378225 @12:34:53.86 -> Handoff.open @12:34:53.89 Handoff.select :open @12:34:53.94 Valve::Manifold::Error in na -- Handoff Valve::Manifold has no selection: open @12:34:58.09 -> Syringe.psi 15.9481402393963 @12:35:04.02 -> Handoff.waste @12:35:04.04 Handoff.select :waste Handoff Valve::Manifold selects waste @12:35:07.32 -> Syringe.psi 15.7745697989029 @12:35:13.54 -> Syringe.psi 15.9210198580692 @12:35:17.70 -> Syringe.psi 15.9589883919271 @12:36:06.17 -> exit @12:36:06.68 Session Ended @12:44:41.69 Session Begun @12:44:48.49 -> Handoff Handoff Valve::Manifold selects waste @12:44:59.72 -> PV Plunge Valve (PV) at puckTop @12:45:10.49 -> PV.pBottom @12:45:10.53 Plunge.seek :pBottom Plunge Valve (PV) at puckBottom @12:45:17.30 -> Handoff.waste @12:45:17.32 Handoff.select :waste Handoff Valve::Manifold selects waste @12:45:20.73 -> Syringe.psi 16.2030718238709 @12:46:09.14 -> Cart.dry [52] @12:46:26.09 -> PhytoSampler.goalVolume 100 @12:46:37.53 -> start {fire lysis} @12:46:37.69 Cartridge::None in detached from na -- No dry microSPR_phyto cartridges remain # @12:46:45.45 -> slot 52 {52 => { Type: :unknown, State: :dry}} @12:47:23.94 -> lysis=:lyseHold_phyto :lyseHold_phyto @12:47:42.50 -> slot 52=>[:dry,:lyseHold_phyto] {52 => { Type: :lyseHold_phyto, State: :dry}} @12:48:01.41 -> start {fire lysis} @12:48:01.58 Selecting Cartridge 52 # @12:48:01.82 Gate.power :heat,:OFF @12:48:01.84 Gate.power :cartridge,:OFF @12:48:01.98 PinPort.in :unlocking @12:48:02.07 PinPort.seek :clear @12:48:02.92 Carousel.in :default @12:48:03.08 Carousel.home.seek 22 @12:48:03.44 Archive.in :unlocking @12:48:03.59 Archive.seek :clear @12:48:03.73 Air.in :unlocking @12:48:03.91 Air.seek :clear @12:48:04.01 Lysis2.in :unlocking @12:48:04.12 Lysis2.seek :clear @12:48:08.90 Lysis1.in :unlocking @12:48:09.06 Lysis1.seek :clear @12:48:09.16 Plunge.in :unlocking @12:48:09.25 Plunge.seek :clear @12:48:11.29 Carousel.seek 52 @12:48:17.76 Carousel.reconfigure :align @12:48:17.91 Lysis1.in :fast @12:48:17.98 Lysis1.seek :locked @12:48:20.64 Plunge.in :default @12:48:20.71 Plunge.seek :locked @12:48:20.90 Air.in :fast @12:48:20.99 Air.seek :locked @12:48:22.08 Carousel.reconfigure :locked @12:48:22.26 Powering cartridge bus @12:48:24.92 Gate.setIR 1750,5000,0,6 @12:48:25.05 Gate.setIR 0,5000,0,6 @12:48:25.14 PuckHeater.configure :default @12:48:25.23 Gate.power :heat,:ON,limit to 5000mA @12:48:25.44 Mounted lysis Cartridge board#J59981 @12:48:25.49 Using default cartridge heat loss curve for HeatingFunctionInCarousel @12:48:25.83 Preparing to filter lyseHold_phyto cartridge in slot 52 @12:48:26.10 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:48:32.72 Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) @12:48:32.74 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @12:48:32.81 PhaseSeparator.in :filling @12:48:32.89 PhaseSeparator.hold @12:48:32.98 Gate.power :sampler,:ON @12:48:39.98 Head pressure reached 3.31psi in 8 seconds @12:48:40.03 Air.in :default @12:48:40.21 Air.seek :sealed @12:48:40.26 Priming sample loop w/200ml, bypass w/10ml @12:48:40.32 SamplePump.setPosition! 0ml @12:48:40.48 Bypass.open @12:48:42.78 Intake.open @12:48:44.39 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:48:47.02 Vacuum tare is 53% of full scale at 26C (drifted 2%) @12:48:47.20 Abs.in :default @12:48:47.29 Abs.hold @12:48:47.39 PhaseSeparator.in :full @12:48:47.47 PhaseSeparator.hold @12:48:47.72 Waiting for sampler to finish priming... @12:48:47.74 delayUntil "12:49:33.00" @12:49:33.07 SamplePump.in :start @12:49:33.16 SamplePump.seek 10ml,1:10 @12:49:33.76 SamplePump.configure :prime @12:49:46.72 Abs.stop @12:49:46.79 PhaseSeparator.stop @12:49:46.86 SamplePump.setPosition! 0ml @12:49:46.93 Exhaust.open @12:49:49.13 Bypass.close @12:49:51.48 Abs.in :default @12:49:51.57 Abs.hold @12:49:51.67 PhaseSeparator.hold @12:49:52.02 SamplePump.in :start @12:49:52.12 SamplePump.seek 200ml,13:50 @12:49:52.71 SamplePump.configure :prime @12:54:14.90 Abs.stop @12:54:14.98 PhaseSeparator.stop @12:54:15.07 Bypass.open @12:54:16.61 Intake.close @12:54:18.23 Toroid.in :hold @12:54:18.31 Toroid.seek :crack @12:54:20.85 Toroid.seek :cartridge @12:54:23.00 Toroid.in :default @12:54:23.13 Toroid.seek :clear @12:54:27.13 Pressurizing cartridge to 14psi above 15.1psia @12:54:27.16 SamplePump.setPosition! 0ml @12:54:27.25 Intake.open @12:54:28.72 Bypass.close @12:54:30.27 Exhaust.close @12:54:32.01 Abs.in :default @12:54:32.10 Abs.hold @12:54:32.20 PhaseSeparator.hold @12:54:32.51 SamplePump.in :charge @12:54:32.60 SamplePump.seek 2.3ml,38 seconds @12:54:43.31 Abs.stop @12:54:43.40 PhaseSeparator.stop @12:54:43.49 Intake.close @12:54:45.04 Pressurized cartridge with 0.471ml to 14.6psi @12:54:45.05 Isolated @26.4psia (11.3psi) @12:54:45.11 Verifying cartridge holds at least 0.65..0.85 of 26.4psia @12:54:45.17 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:54:49.78 Cartridge pressure rose 4% to 27.5psia @12:54:49.92 Sampling 100.0ml @12:54:49.94 SamplePump.setPosition! 0ml @12:54:50.03 Exhaust.open @12:54:51.50 Intake.open @12:54:53.23 Abs.in :default @12:54:53.35 Abs.hold @12:54:53.47 PhaseSeparator.hold @12:54:53.83 SamplePump.in :start @12:54:53.94 SamplePump.seek 100ml,6:04 @12:54:54.52 SamplePump.configure :sample @12:54:56.92 Cartridge 52 filtering 2/100ml at 0.70ml/s with 9.2psi vacuum @12:55:56.98 Cartridge 52 filtering 44/100ml at 0.70ml/s with 9.7psi vacuum 4.7m deep (21.6psia) @12:56:56.97 Cartridge 52 filtering 85/100ml at 0.69ml/s with 11.1psi vacuum @12:57:18.48 Abs.stop @12:57:18.54 PhaseSeparator.stop @12:57:18.67 Intake.close @12:57:18.76 Exhaust.close @12:57:18.84 Handoff.select :close @12:57:19.62 Toroid.in :hold @12:57:19.70 Toroid.seek :bypass @12:57:23.58 Toroid.in :default @12:57:23.71 Toroid.seek :clear @12:57:27.32 Sampled 100.0ml @12:57:28.16 Handoff.select :waste @12:57:28.28 Gate.power :heat,:OFF @12:57:28.30 Gate.power :cartridge,:OFF @12:57:28.47 Carousel.in :default @12:57:28.62 Carousel.home.seek 52 @12:57:28.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:57:29.01 Plunge.seek :locked @12:57:29.29 Air.in :unlocking @12:57:29.42 Air.seek :clear @12:57:29.55 Lysis1.in :unlocking @12:57:29.71 Lysis1.seek :clear @12:57:30.67 Gate.power :sampler,:OFF @12:57:31.16 Handoff.select :close @12:57:31.76 Gate.power :motor,:OFF @12:57:31.78 Gate.power :analytic1,:OFF @12:57:31.81 Gate.power :core,:OFF @12:57:31.84 Gate.power :main,:OFF @12:57:31.87 Safely stopped and ready to power off /home/esp/esp2/utils/auv/cmd.rb:490: warning: Object#type is deprecated; use Object#class @12:57:32.54 BusOff in PROCESSING -- core bus was not powered while reading Storage Carousel (SC)'s status @12:57:32.56 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" @13:01:13.54 -> Power {analytic1: false, cartridge: false, core: false, heat: false, main: false, motor: false, sampler: false, skimmer: false} @13:01:36.99 -> Syringe.psi @13:01:37.04 BusOff in na -- core bus was not powered while reading Air Syringe (AirS)'s status @13:01:46.05 -> ESP.configure @13:01:46.11 Gate.power :sampler,:OFF,limit to 3500mA @13:01:46.13 Gate.power :skimmer,:OFF,limit to 3500mA @13:01:46.16 Gate.power :main,:ON,limit to 5000mA @13:01:46.19 Gate.power :analytic1,:ON,limit to 4000mA @13:01:46.29 Powering core bus @13:01:49.07 Plunge.configure :default @13:01:49.18 SamplePump.configure :default @13:01:49.29 Toroid.configure :default @13:01:49.39 Carousel.configure :default @13:01:49.50 Abs.configure :default @13:01:49.60 PhaseSeparator.configure :full @13:01:49.71 Archive.configure :default @13:01:49.82 Air.configure :default @13:01:49.90 Lysis1.configure :default @13:01:49.97 Lysis2.configure :default @13:01:50.33 PinPort.configure :default @13:01:50.46 All elf controllers running firmware version 4.94 @13:01:50.48 Gate.power :motor,:ON,limit to 5000mA @13:01:53.57 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @13:01:53.60 AnalyticModule::Unknown in na -- SPR @13:01:56.90 -> Syringe.psi 0.527491416811955 @13:11:57.68 Session Ended @13:43:03.78 Session Begun @13:43:16.33 -> exit @13:43:16.39 Session Ended @13:45:15.41 Session Begun @13:45:28.65 -> Cart.unlock @13:45:28.78 Slide::Error in benjamin -- Handoff Pin Port (PP) Not yet Homed! @13:45:48.42 -> ESP.ready! @13:45:48.67 Plunge.home.jog 0 @13:45:49.97 PinPort.home.jog 0 @13:45:50.03 Lysis2.home.jog 0 @13:45:50.07 Air.home.jog 0 @13:45:50.12 Carousel.home.jog 0 @13:45:50.20 Archive.home.jog 0 @13:45:50.26 Toroid.home.jog 0 @13:45:50.31 Lysis1.home.jog 0 @13:45:51.21 Plunge.in :homing @13:45:51.29 Archive.in :default @13:45:51.58 Archive.home.jog 0 @13:45:51.67 Plunge.jog 1460 @13:45:51.71 Lysis2.in :homing @13:45:51.77 Air.in :homing @13:45:51.81 Toroid.in :homing @13:45:51.85 Lysis1.in :homing @13:45:51.88 PinPort.in :homing @13:45:52.24 Lysis2.jog 1623 @13:45:52.35 Lysis1.jog 1600 @13:45:52.43 Toroid.jog 1359 @13:45:52.46 Air.jog 1427 @13:45:52.51 PinPort.jog 1231 @13:45:53.00 Archive.in :homing @13:45:53.09 Archive.jog 1595 @13:45:53.24 Plunge.setRawPosition! 50 @13:45:53.32 Plunge.seek :home @13:45:53.64 Plunge.in :default @13:45:53.83 Lysis2.setRawPosition! 50 @13:45:53.91 Lysis2.seek :home @13:45:54.05 Lysis1.setRawPosition! 50 @13:45:54.07 Air.setRawPosition! 0 @13:45:54.18 Lysis1.seek :home @13:45:54.25 Air.seek :home @13:45:54.59 Lysis2.in :default @13:45:54.62 Air.in :default @13:45:54.66 PinPort.setRawPosition! 3150 @13:45:54.70 Archive.setRawPosition! 50 @13:45:54.94 PinPort.seek :home @13:45:54.97 Archive.seek :home @13:45:55.19 Lysis1.in :default @13:45:55.35 Archive.in :default @13:45:57.21 Toroid.setRawPosition! 0 @13:45:57.28 Toroid.seek :home @13:45:57.40 Toroid.in :default @13:45:59.41 PinPort.in :default @13:45:59.78 Toroid.seek :clear @13:45:59.97 PinPort.in :unlocking @13:46:00.06 PinPort.seek :clear @13:46:03.43 Air.seek :clear @13:46:03.55 Lysis1.seek :clear @13:46:04.30 Lysis2.seek :clear @13:46:05.14 Archive.seek :clear @13:46:05.95 Carousel.in :default @13:46:06.09 Carousel.home.seek 52 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 52 Abs = Absolute Pressure (Leak) Sensor @18psia PhaseSeparator = Intake Head Pressure @0.02psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:46:19.06 -> SC.to 22 @13:46:19.09 Carousel.seek 22 Storage Carousel (SC) at 22 @13:53:05.84 Session Begun @13:53:09.38 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 22 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000.0}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 52 => { Type: :lyseHold_phyto, State: :processing, filtered: 100.0}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @13:53:39.10 -> exit @13:53:39.13 Session Ended @13:56:09.29 Session Begun @13:56:12.10 -> progress >>> Thread[:MAIN] is sleeping <<< @11:53:10 Waiting for espclients... >>> Thread["detached from na"] is sleeping <<< @12:48:01 Selecting Cartridge 52 >>> Thread[:PROCESSING] is suspended <<< @12:57:28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:56:35.47 -> Thread["detached from na"].abort @13:56:35.52 Cmd::Error in detached from na -- Not Processing # @13:56:35.61 Thread::Aborted in detached from na -- "Aborted by preston" @13:56:35.70 Waiting to Resume from Checkpoint ""Aborted by preston"" @13:56:56.03 -> Thread[:PROCESSING].abort @13:56:56.07 Thread::Aborted in PROCESSING -- "Aborted by preston" # @13:56:56.15 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" @13:57:12.71 -> progress >>> Thread[:MAIN] is sleeping <<< @11:53:10 Waiting for espclients... >>> Thread["detached from na"] is suspended <<< @12:48:01 Selecting Cartridge 52 @13:56:32 "Aborted by preston" >>> Thread[:PROCESSING] is suspended <<< @12:57:28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:57:41.07 -> MainThread.exit # @13:57:41.26 ThreadError in preston -- killed thread @13:57:41.36 Session Ended @13:57:41.40 Session Ended @13:57:54.67EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:57:56.33 >BREAK< ESP3 Gateway v1.44 for core bus @13:57:56.37 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:57:59.87 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:58:00.57 Sea pressure (Abs) tare is 6% of full scale @13:58:03.02 Polling Can every 2:00 @13:58:03.43 Waiting for espclients... @13:58:04.74 Session Begun @13:58:22.37 -> Cart.type=:lyseHold_phyto :lyseHold_phyto @13:58:28.49 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 22 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 52 => { Type: :lyseHold_phyto, State: :processing, filtered: 100}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @13:58:34.07 -> SC Storage Carousel (SC) at 22 @13:58:40.09 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 22 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 52 => { Type: :lyseHold_phyto, State: :processing, filtered: 100}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @14:00:24.58 -> PhytoSampler.goalVolume=50; PhytoSampler.primeVolume=25 25 @14:01:08.40 -> SC Storage Carousel (SC) at 22 @14:01:31.45 -> slot 52 {52 => { Type: :lyseHold_phyto, State: :processing, filtered: 100}} @14:01:45.67 -> slot 52=>:dry {52 => { Type: :lyseHold_phyto, State: :dry, filtered: 100}} @14:01:57.46 -> lysis :microSPR_phyto @14:02:24.24 -> lysis=:lyseHold_phyto :lyseHold_phyto @14:03:01.46 -> start {fire lysis} # @14:03:01.82 Gate.power :sampler,:OFF,limit to 3500mA @14:03:01.84 Gate.power :skimmer,:OFF,limit to 3500mA @14:03:01.90 Gate.power :analytic1,:ON,limit to 4000mA @14:03:02.62 Plunge.configure :default @14:03:02.79 SamplePump.configure :default @14:03:02.97 Toroid.configure :default @14:03:03.06 Toroid.hold @14:03:03.17 Toroid.configure :default @14:03:03.33 Carousel.configure :default @14:03:03.42 Carousel.hold @14:03:03.53 Carousel.configure :default @14:03:03.70 Abs.configure :default @14:03:04.15 PhaseSeparator.configure :default @14:03:04.32 Archive.configure :default @14:03:04.45 Archive.hold @14:03:04.56 Archive.configure :default @14:03:04.72 Air.configure :default @14:03:04.82 Air.hold @14:03:04.92 Air.configure :default @14:03:05.06 Lysis1.configure :default @14:03:05.15 Lysis1.hold @14:03:05.25 Lysis1.configure :default @14:03:05.39 Lysis2.configure :default @14:03:05.48 Lysis2.hold @14:03:05.57 Lysis2.configure :default @14:03:06.00 PinPort.configure :default @14:03:06.08 PinPort.hold @14:03:06.19 PinPort.configure :default @14:03:06.27 All elf controllers running firmware version 4.94 @14:03:07.37 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @14:03:07.41 AnalyticModule::Unknown in detached from preston -- SPR @14:03:08.10 Toroid.seek :clear @14:03:08.31 Air.seek :clear @14:03:08.47 Lysis1.seek :clear @14:03:08.59 Lysis2.seek :clear @14:03:08.71 Archive.seek :clear @14:03:08.82 Carousel.in :default @14:03:08.97 Carousel.home.seek 22 @14:03:09.74 Selecting Cartridge 52 @14:03:10.08 Carousel.seek 52 @14:03:16.55 Carousel.reconfigure :align @14:03:16.70 Lysis1.in :fast @14:03:16.79 Lysis1.seek :locked @14:03:19.44 Plunge.seek :locked @14:03:19.63 Air.in :fast @14:03:19.73 Air.seek :locked @14:03:20.82 Carousel.reconfigure :locked @14:03:21.00 Powering cartridge bus @14:03:23.66 Gate.setIR 1750,5000,0,6 @14:03:23.79 Gate.setIR 0,5000,0,6 @14:03:23.88 PuckHeater.configure :default @14:03:23.96 Gate.power :heat,:ON,limit to 5000mA @14:03:24.22 Mounted lysis Cartridge board#J59981 @14:03:24.28 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:03:24.66 Preparing to filter lyseHold_phyto cartridge in slot 52 @14:03:24.70 Cartridge already filtered 100.0ml @14:03:25.05 Cartridge::Error in detached from preston -- Cannot process dry cartridge 52 @14:03:25.06 Waiting to Resume from Checkpoint "Selecting Cartridge 52" @14:04:18.02 -> Sampler.reset @14:04:52.49 -> Thread["detached from preston"].abort @14:04:52.52 Thread::Aborted in detached from preston -- "Aborted by preston" # @14:04:52.58 Waiting to Resume from Checkpoint "Selecting Cartridge 52" @14:05:06.74 -> start {fire lysis} @14:05:06.91 Selecting Cartridge 52 # @14:05:07.62 Preparing to filter lyseHold_phyto cartridge in slot 52 @14:05:07.66 Cartridge already filtered 100.0ml @14:05:08.00 Cartridge::Error in detached from preston#2 -- Cannot process dry cartridge 52 @14:05:08.02 Waiting to Resume from Checkpoint "Selecting Cartridge 52" @14:05:17.46 -> Cart.unlock @14:05:17.52 Gate.power :heat,:OFF @14:05:17.53 Gate.power :cartridge,:OFF @14:05:17.65 Carousel.in :default @14:05:17.80 Carousel.home.seek 52 @14:05:18.25 Air.in :unlocking @14:05:18.42 Air.seek :clear @14:05:19.51 Lysis1.in :unlocking @14:05:19.66 Lysis1.seek :clear @14:05:19.79 Plunge.in :unlocking @14:05:19.89 Plunge.seek :clear @14:05:47.38 -> slot 52=>nil {52 => nil} @14:06:17.14 -> slot 52=>:lyseHold_phyto {52 => { Type: :lyseHold_phyto, State: :dry}} @14:06:23.46 -> start {fire lysis} @14:06:23.65 Selecting Cartridge 52 # @14:06:24.45 Carousel.seek 52 @14:06:24.75 Carousel.reconfigure :align @14:06:24.91 Lysis1.in :fast @14:06:24.99 Lysis1.seek :locked @14:06:27.65 Plunge.in :default @14:06:27.78 Plunge.seek :locked @14:06:27.93 Air.in :fast @14:06:28.01 Air.seek :locked @14:06:29.11 Carousel.reconfigure :locked @14:06:29.29 Powering cartridge bus @14:06:31.96 Gate.setIR 1750,5000,0,6 @14:06:32.09 Gate.setIR 0,5000,0,6 @14:06:32.18 PuckHeater.configure :default @14:06:32.27 Gate.power :heat,:ON,limit to 5000mA @14:06:32.51 Mounted lysis Cartridge board#J59981 @14:06:32.57 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:06:32.91 Preparing to filter lyseHold_phyto cartridge in slot 52 @14:06:33.19 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:06:39.82 Intake Head Pressure tare is 0% of full scale at 26C (drifted 1%) @14:06:39.84 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:06:39.90 PhaseSeparator.in :filling @14:06:39.97 PhaseSeparator.hold @14:06:40.06 Gate.power :sampler,:ON @14:06:47.12 Head pressure reached 3.51psi in 8 seconds @14:06:47.16 Air.in :default @14:06:47.34 Priming sample loop w/25ml, bypass w/10ml @14:06:47.37 Air.seek :sealed @14:06:47.43 SamplePump.setPosition! 0ml @14:06:47.58 Exhaust.close @14:06:47.71 Bypass.open @14:06:50.04 Intake.open @14:06:51.63 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:06:54.26 Vacuum tare is 54% of full scale at 26C (drifted 2%) @14:06:54.47 Abs.in :default @14:06:54.54 Abs.hold @14:06:55.08 PhaseSeparator.in :full @14:06:55.15 PhaseSeparator.hold @14:06:55.40 Waiting for sampler to finish priming... @14:06:55.42 delayUntil "14:07:40.08" @14:07:40.24 SamplePump.in :start @14:07:40.33 SamplePump.seek 10ml,1:10 @14:07:40.92 SamplePump.configure :prime @14:07:53.92 Abs.stop @14:07:53.99 PhaseSeparator.stop @14:07:54.07 SamplePump.setPosition! 0ml @14:07:54.14 Exhaust.open @14:07:56.39 Bypass.close @14:07:58.77 Abs.in :default @14:07:58.85 Abs.hold @14:07:58.97 PhaseSeparator.hold @14:07:59.32 SamplePump.in :start @14:07:59.42 SamplePump.seek 25ml,2:10 @14:08:00.01 SamplePump.configure :prime @14:08:32.65 Abs.stop @14:08:32.71 PhaseSeparator.stop @14:08:32.83 Bypass.open @14:08:34.41 Intake.close @14:08:36.04 Toroid.in :hold @14:08:36.12 Toroid.seek :crack @14:08:38.62 Toroid.seek :cartridge @14:08:40.77 Toroid.in :default @14:08:40.86 Toroid.seek :clear @14:08:44.86 Pressurizing cartridge to 14psi above 15.0psia @14:08:44.88 SamplePump.setPosition! 0ml @14:08:44.96 Intake.open @14:08:46.48 Bypass.close @14:08:48.04 Exhaust.close @14:08:49.75 Abs.in :default @14:08:49.83 Abs.hold @14:08:49.93 PhaseSeparator.hold @14:08:50.18 SamplePump.in :charge @14:08:50.27 SamplePump.seek 2.3ml,38 seconds @14:08:59.03 Abs.stop @14:08:59.11 PhaseSeparator.stop @14:08:59.21 Intake.close @14:09:00.80 Pressurized cartridge with 0.312ml to 18.9psi @14:09:00.82 Isolated @22.1psia (7.1psi) @14:09:00.87 Verifying cartridge holds at least 0.65..0.85 of 22.1psia @14:09:00.93 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:09:05.54 Cartridge pressure fell 1% to 21.9psia @14:09:05.66 Sampling 50.0ml @14:09:05.69 SamplePump.setPosition! 0ml @14:09:05.78 Exhaust.open @14:09:07.27 Intake.open @14:09:08.98 Abs.in :default @14:09:09.07 Abs.hold @14:09:09.18 PhaseSeparator.hold @14:09:09.55 SamplePump.in :start @14:09:09.65 SamplePump.seek 50ml,3:17 @14:09:10.24 SamplePump.configure :sample @14:09:12.64 Cartridge 52 filtering 2/50ml at 0.71ml/s with 13.5psi vacuum @14:10:12.69 Cartridge 52 filtering 45/50ml at 0.72ml/s with 15.3psi vacuum @14:10:20.02 Abs.stop @14:10:20.10 PhaseSeparator.stop @14:10:20.20 Intake.close @14:10:20.31 Exhaust.close @14:10:20.57 Toroid.in :hold @14:10:20.65 Toroid.seek :bypass @14:10:24.27 Toroid.in :default @14:10:24.37 Toroid.seek :clear @14:10:27.99 Sampled 50.0ml @14:10:28.57 Handoff.select :waste @14:10:29.11 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:10:31.19 Gate.power :sampler,:OFF @14:10:35.74 Syringe tare is 50% of full scale at 26C (drifted -1%) @14:10:35.77 PinPort.in :locking @14:10:35.86 PinPort.seek :locked @14:10:36.72 Archive.in :fast @14:10:36.78 Lysis2.in :fast @14:10:36.87 Lysis1.seek 1250uL @14:10:36.97 Archive.seek :empty @14:10:37.09 Lysis2.seek :empty @14:10:46.16 Evacuating puck with filter bubble point of 20psi @14:10:46.19 PinPort.seek :locked @14:10:46.32 Handoff.select :waste @14:10:46.41 Plunge.seek :puckBottom @14:10:47.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:10:54.34 Air syringe pumping up to 1 strokes to reach 4psi @14:10:54.37 Air stroke #1 of 1 @14:10:54.53 Air.in :pressurize @14:10:54.61 Air.seek :pressurized @14:10:59.88 0.9psi after pressurize @14:10:59.90 Air.in :compress @14:11:00.01 Air.seek :evac @14:11:01.10 Reached 4.3 of 4.0psi after pumping 1457uL (0.82 strokes) @14:11:01.12 Air.in :default @14:11:01.22 Air.hold @14:11:01.33 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:11:21.92 Stabilized at 11.1psi after evac to waste @14:11:21.93 Handoff.select :blocked @14:11:22.97 Handoff.select :waste @14:11:24.03 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:11:32.63 Handoff.select :blocked @14:11:33.16 Plunge.seek :puckTop @14:11:34.07 Overpressure in PROCESSING -- Waste port clogged (11.3psi) @14:11:34.09 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Syringe to stabilize +/-0.20psi" @14:12:36.17 -> Syringe.psi 12.248920226381 @14:12:41.81 -> Handoff.waste @14:12:41.86 Handoff.select :waste Handoff Valve::Manifold selects waste @14:12:45.78 -> Syringe.psi 12.1024701672147 @14:12:49.37 -> Handoff.waste @14:12:49.40 Handoff.select :waste Handoff Valve::Manifold selects waste @14:12:52.18 -> Syringe.psi 12.1024701672147 @14:12:56.34 -> Handoff.waste @14:12:56.40 Handoff.select :waste Handoff Valve::Manifold selects waste @14:13:00.17 -> PV Plunge Valve (PV) at puckTop @14:13:06.02 -> PV.pBottome @14:13:06.07 NoMethodError in preston -- undefined method `pBottome' for Plunge:Slide @14:13:08.50 -> PV.pBottom @14:13:08.53 Plunge.seek :pBottom Plunge Valve (PV) at puckBottom @14:13:16.02 -> PV.pTop @14:13:16.07 Plunge.seek :pTop Plunge Valve (PV) at puckTop @14:13:20.81 -> PV.pBottom @14:13:20.85 Plunge.seek :pBottom Plunge Valve (PV) at puckBottom @14:13:32.09 -> Handoff.waste @14:13:32.12 Handoff.select :waste Handoff Valve::Manifold selects waste @14:14:14.67 -> Cart.unlock @14:14:14.73 Gate.power :heat,:OFF @14:14:14.75 Gate.power :cartridge,:OFF @14:14:14.86 PinPort.in :unlocking @14:14:14.95 PinPort.seek :clear @14:14:15.80 Carousel.in :default @14:14:15.94 Carousel.home.seek 52 @14:14:16.31 Archive.in :unlocking @14:14:16.51 Archive.seek :clear @14:14:16.64 Air.in :unlocking @14:14:16.78 Air.seek :clear @14:14:16.92 Lysis2.in :unlocking @14:14:17.01 Lysis2.seek :clear @14:14:21.77 Lysis1.in :unlocking @14:14:21.95 Lysis1.seek :clear @14:14:22.06 Plunge.in :unlocking @14:14:22.15 Plunge.seek :clear @14:14:35.63 -> MainThread.exit @14:14:35.75 Handoff.select :close # @14:14:36.38 Session Ended @14:42:44.15EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:42:47.94 >BREAK< ESP3 Gateway v1.44 for core bus @14:42:47.97 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:42:51.86 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:42:52.59 Sea pressure (Abs) tare is 6% of full scale @14:42:55.64 Polling Can every 2:00 @14:42:55.73 Waiting for espclients... @14:42:56.78 Session Begun @14:44:20.73 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, 22 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 52 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @14:44:27.38 -> slot 22 {22 => { Type: :lyseHold_phyto, State: :lysing, filtered: 1000}} @14:44:45.94 -> slot 22=>[:lyseHold_phyto,:dry] {22 => { Type: :lyseHold_phyto, State: :dry}} @14:44:49.30 -> slot 52 {52 => { Type: :lyseHold_phyto, State: :lysing, filtered: 50}} @14:44:58.26 -> slot 52=>nil {52 => nil} @14:45:07.49 -> slot 52=>[:lyseHold_phyto,:dry] {52 => { Type: :lyseHold_phyto, State: :dry}} @14:45:11.75 -> Cart.dry [52,22] @14:45:30.26 -> lysis=lyseHold_phyto :lyseHold_phyto @14:45:37.98 -> lysis :lyseHold_phyto @14:46:34.73 Session Begun @14:47:28.15 -> PhytoSampler.goalVolume=100 100 @14:47:37.41 -> PhytoSampler.primeVolume=25 25 @14:47:42.37 Session Begun @14:47:47.14 -> start {fire lysis} @14:47:47.35 Cartridge::None in detached from han -- No dry microSPR_phyto cartridges remain # @14:48:36.24 -> lysis=:lyseHold_phyto :lyseHold_phyto @14:48:42.45 -> lysis :lyseHold_phyto @14:48:54.75 -> start {fire lysis} # @14:48:55.00 Gate.power :sampler,:OFF,limit to 3500mA @14:48:55.08 Gate.power :skimmer,:OFF,limit to 3500mA @14:48:55.16 Gate.power :main,:ON,limit to 5000mA @14:48:55.26 Gate.power :analytic1,:ON,limit to 4000mA @14:48:55.51 Powering core bus @14:48:58.30 Plunge.configure :default @14:48:58.45 SamplePump.configure :default @14:48:58.58 Toroid.configure :default @14:48:58.72 Carousel.configure :default @14:48:58.86 Abs.configure :default @14:48:59.00 PhaseSeparator.configure :default @14:48:59.39 Archive.configure :default @14:48:59.54 Air.configure :default @14:48:59.65 Lysis1.configure :default @14:48:59.76 Lysis2.configure :default @14:48:59.90 PinPort.configure :default @14:49:00.06 All elf controllers running firmware version 4.94 @14:49:00.14 Gate.power :motor,:ON,limit to 5000mA @14:49:02.65 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @14:49:02.71 AnalyticModule::Unknown in detached from han -- SPR @14:49:03.10 Plunge.home.jog 0 @14:49:03.75 Lysis2.home.jog 0 @14:49:03.94 Air.home.jog 0 @14:49:04.08 Carousel.home.jog 0 @14:49:04.15 -> exot @14:49:04.23 PinPort.home.jog 0 @14:49:04.28 Lysis1.home.jog 0 @14:49:04.36 Toroid.home.jog 0 @14:49:04.48 Archive.home.jog 0 :exot @14:49:05.56 Plunge.in :homing @14:49:05.82 Plunge.jog 582 @14:49:05.92 Lysis2.in :homing @14:49:05.97 Air.in :homing @14:49:06.07 Toroid.in :homing @14:49:06.15 PinPort.in :homing @14:49:06.21 Archive.in :homing @14:49:06.29 Lysis1.in :homing @14:49:06.67 Lysis2.jog 1619 @14:49:06.71 Air.jog 1429 @14:49:06.95 PinPort.jog 1225 @14:49:07.00 Lysis1.jog 1595 @14:49:07.08 Archive.jog 309 @14:49:07.23 Toroid.jog 1362 @14:49:07.61 Plunge.setRawPosition! 50 @14:49:07.74 Plunge.seek :home @14:49:08.09 Plunge.in :default @14:49:08.12 Archive.setRawPosition! 50 @14:49:08.35 Archive.seek :home @14:49:08.50 Lysis2.setRawPosition! 50 @14:49:08.57 Air.setRawPosition! 0 @14:49:08.78 Lysis2.seek :home @14:49:08.83 Air.seek :home @14:49:09.08 Lysis1.setRawPosition! 50 @14:49:09.21 Archive.in :default @14:49:09.29 Air.in :default @14:49:09.36 Lysis1.seek :home @14:49:10.16 PinPort.setRawPosition! 3150 @14:49:10.18 Lysis2.in :default @14:49:10.40 PinPort.seek :home @14:49:10.58 Lysis1.in :default @14:49:12.06 Toroid.setRawPosition! 0 @14:49:12.18 Toroid.seek :home @14:49:12.32 Toroid.in :default @14:49:14.83 PinPort.in :default @14:49:15.24 Toroid.seek :clear @14:49:15.46 PinPort.in :unlocking @14:49:15.57 PinPort.seek :clear @14:49:18.95 Air.seek :clear @14:49:19.12 Lysis1.seek :clear @14:49:19.89 Lysis2.seek :clear @14:49:20.77 Archive.seek :clear @14:49:21.63 Carousel.in :default @14:49:21.82 Carousel.home.seek 52 @14:49:22.18 Selecting Cartridge 52 @14:49:22.54 Carousel.seek 52 @14:49:22.90 Carousel.reconfigure :align @14:49:23.08 Lysis1.in :fast @14:49:23.20 Lysis1.seek :locked @14:49:25.88 Plunge.seek :locked @14:49:26.14 Air.in :fast @14:49:26.26 Air.seek :locked @14:49:27.36 Carousel.reconfigure :locked @14:49:27.60 Powering cartridge bus @14:49:28.80 -> exit @14:49:28.84 Session Ended @14:49:30.26 Gate.setIR 1750,5000,0,6 @14:49:30.40 Gate.setIR 0,5000,0,6 @14:49:30.50 PuckHeater.configure :default @14:49:30.61 Gate.power :heat,:ON,limit to 5000mA @14:49:30.89 Mounted lysis Cartridge board#J59981 @14:49:30.95 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:49:31.34 Preparing to filter lyseHold_phyto cartridge in slot 52 @14:49:31.63 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:49:38.25 Intake Head Pressure tare is 0% of full scale at 23C (drifted 1%) @14:49:38.28 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:49:38.37 PhaseSeparator.in :filling @14:49:38.46 PhaseSeparator.hold @14:49:38.58 Gate.power :sampler,:ON @14:49:41.71 -> exit @14:49:41.73 Session Ended @14:49:45.53 Head pressure reached 3.44psi in 8 seconds @14:49:45.56 Air.in :default @14:49:45.77 Priming sample loop w/25ml, bypass w/10ml @14:49:45.81 SamplePump.setPosition! 0ml @14:49:45.86 Air.seek :sealed @14:49:46.00 Bypass.open @14:49:48.42 Intake.open @14:49:50.06 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:49:52.69 Vacuum tare is 54% of full scale at 23C (drifted 2%) @14:49:52.88 Abs.in :default @14:49:52.96 Abs.hold @14:49:53.06 PhaseSeparator.in :full @14:49:53.14 PhaseSeparator.hold @14:49:53.40 Waiting for sampler to finish priming... @14:49:53.42 delayUntil "14:50:38.60" @14:50:38.67 SamplePump.in :start @14:50:38.77 SamplePump.seek 10ml,1:10 @14:50:39.36 SamplePump.configure :prime @14:50:52.33 Abs.stop @14:50:52.41 PhaseSeparator.stop @14:50:52.48 SamplePump.setPosition! 0ml @14:50:52.55 Exhaust.open @14:50:54.82 Bypass.close @14:50:57.28 Abs.in :default @14:50:57.36 Abs.hold @14:50:57.47 PhaseSeparator.hold @14:50:57.83 SamplePump.in :start @14:50:57.93 SamplePump.seek 25ml,2:10 @14:50:58.52 SamplePump.configure :prime @14:51:05.96 Session Ended @14:51:31.21 Abs.stop @14:51:31.25 PhaseSeparator.stop @14:51:31.33 Bypass.open @14:51:32.84 Intake.close @14:51:34.45 Toroid.in :hold @14:51:34.50 Toroid.seek :crack @14:51:36.97 Toroid.seek :cartridge @14:51:39.09 Toroid.in :default @14:51:39.14 Toroid.seek :clear @14:51:43.62 Pressurizing cartridge to 14psi above 15.4psia @14:51:43.64 SamplePump.setPosition! 0ml @14:51:43.69 Intake.open @14:51:45.18 Bypass.close @14:51:46.68 Exhaust.close @14:51:48.35 Abs.in :default @14:51:48.40 Abs.hold @14:51:48.49 PhaseSeparator.hold @14:51:48.74 SamplePump.in :charge @14:51:48.80 SamplePump.seek 2.3ml,38 seconds @14:52:07.04 Abs.stop @14:52:07.09 PhaseSeparator.stop @14:52:07.15 Intake.close @14:52:08.72 Pressurized cartridge with 1.391ml to 14.2psi @14:52:08.74 Isolated @28.5psia (13.1psi) @14:52:08.76 Verifying cartridge holds at least 0.65..0.85 of 28.5psia @14:52:08.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:52:13.47 Sampling 100.0ml @14:52:13.48 SamplePump.setPosition! 0ml @14:52:13.54 Exhaust.open @14:52:15.03 Intake.open @14:52:16.88 Abs.in :default @14:52:16.93 Abs.hold @14:52:17.01 PhaseSeparator.hold @14:52:17.33 SamplePump.in :start @14:52:17.39 SamplePump.seek 100ml,6:04 @14:52:18.03 SamplePump.configure :sample @14:52:20.43 Cartridge 52 filtering 2/100ml at 0.75ml/s with 10.3psi vacuum @14:53:20.48 Cartridge 52 filtering 47/100ml at 0.74ml/s with 9.0psi vacuum @14:54:17.20 Session Begun @14:54:19.54 -> ls :ls @14:54:20.48 Cartridge 52 filtering 91/100ml at 0.75ml/s with 7.5psi vacuum 3.7m deep (20.2psia) @14:54:31.91 Abs.stop @14:54:31.99 PhaseSeparator.stop @14:54:32.14 Intake.close @14:54:32.25 Exhaust.close @14:54:32.47 Toroid.in :hold @14:54:32.56 Toroid.seek :bypass @14:54:36.19 Toroid.in :default @14:54:36.27 Toroid.seek :clear @14:54:40.34 Sampled 100.0ml @14:54:40.93 Handoff.select :waste @14:54:41.43 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:54:43.53 Gate.power :sampler,:OFF @14:54:51.26 -> showlog @14:54:52.55 Syringe tare is 50% of full scale at 23C (drifted -0%) @14:54:52.58 PinPort.in :locking @14:54:52.70 PinPort.seek :locked @14:54:53.57 Archive.in :fast @14:54:53.68 Lysis2.in :fast @14:54:53.75 Lysis1.seek 1250uL @14:54:53.80 Archive.seek :empty @14:54:53.98 Lysis2.seek :empty @14:55:02.55 Evacuating puck with filter bubble point of 20psi @14:55:02.59 PinPort.seek :locked @14:55:02.73 Handoff.select :waste @14:55:02.77 Plunge.seek :puckBottom @14:55:04.11 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:55:11.72 Air syringe pumping up to 1 strokes to reach 4psi @14:55:11.73 Air stroke #1 of 1 @14:55:11.88 Air.in :pressurize @14:55:11.96 Air.seek :pressurized @14:55:17.25 0.3psi after pressurize @14:55:17.28 Air.in :compress @14:55:17.37 Air.seek :evac @14:55:22.74 -> exit @14:55:22.77 Session Ended @14:55:24.05 0.3psi after compress @14:55:25.85 Reached 0.4 of 4.0psi after pumping 1775uL (1.00 strokes) @14:55:25.98 Air.in :default @14:55:26.07 Air.hold @14:55:26.16 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:55:35.75 Stabilized at 0.2psi after evac to waste @14:55:35.77 Handoff.select :blocked @14:55:36.26 Plunge.seek :puckTop @14:55:37.13 Air syringe pumping up to 8 strokes to reach 20psi @14:55:37.14 Air stroke #1 of 8 @14:55:37.23 Air.in :compress @14:55:37.28 Air.seek :evac @14:55:37.43 0.9psi after compress @14:55:48.06 1.1psi after compress (stable) @14:55:48.08 Air.in :gulp @14:55:48.13 Air.seek :topVent @14:55:54.60 0.9psi after gulp @14:56:00.19 1.2psi after gulp (stable) @14:56:00.20 Air stroke #2 of 8 @14:56:00.29 Air.in :pressurize @14:56:00.34 Air.seek :pressurized @14:56:03.40 Reached 1.1 of 20.0psi after pumping 2296uL (1.29 strokes) @14:56:03.42 Air.in :default @14:56:03.47 Air.hold @14:56:03.55 Plunge.seek :puckBottom @14:56:04.41 delay 3 seconds @14:56:07.44 Handoff.select :waste @14:56:07.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:56:14.52 delay 1:00 @14:57:14.54 Handoff.select :blocked @14:57:15.03 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:57:37.63 Handoff.select :waste @14:57:38.12 0.1psi at evacEnd @14:57:38.16 Plunge.seek :pTop @14:57:39.06 delay 5 seconds @14:57:44.09 Air.in :glide @14:57:44.14 Air.seek :fullVent @14:57:56.92 delay 2 seconds @14:57:58.95 Plunge.seek :pBottom @14:57:59.80 Seawater evacuated, Pressure above/below filter released. @14:57:59.82 Plunge.seek :locked @14:58:01.12 Handoff.select :waste @14:58:01.21 Lysis1.in :slow @14:58:01.35 Lysis1.seek 950uL @14:58:15.42 delay 5 seconds @14:58:20.44 Handoff.select :blocked @14:58:20.94 Plunge.seek :puckTop @14:58:21.90 Lysis1.seek 350uL @14:58:49.89 delay 3 seconds @14:58:52.99 Air.in mix with 2.79934 parts Lysis1 @14:58:53.62 Air.seek :evac @14:58:53.69 Lysis1.seek 190uL @14:59:01.32 Air.in :fast @14:59:01.38 Air.seek :full @14:59:07.89 Lysis1.seek :empty @14:59:16.99 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.4psi @14:59:17.01 Plunge.seek :puckBottom @14:59:18.02 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @14.8psi @14:59:18.06 Polling Can every 2:00 @14:59:18.60 PuckHeater.configure :default #ambient 23.4C @14:59:18.65 PuckHeater.seek 81.6C @15:00:40.74 PuckHeater.hold 2:00,81.6C @15:02:40.48 Polling Can every 2:00 @15:02:41.02 PuckHeater.configure :default #ambient 23.5C @15:02:41.08 PuckHeater.seek 80.6C @15:02:41.24 PuckHeater.hold 2:00,80.6C @15:04:40.91 Polling Can every 2:00 @15:04:41.46 PuckHeater.configure :default #ambient 23.5C @15:04:41.51 PuckHeater.hold 8:00,79.5C @15:10:51.52 PuckHeater.configure :default #ambient 23.0C @15:10:51.60 PuckHeater.hold 1:50.01,79.5C @15:12:41.35 Lysate cooked @19.2psi @15:12:41.37 Plunge.seek :puckTop @15:12:42.24 Air syringe pumping up to 5 strokes to reach 40psi @15:12:42.25 Air stroke #1 of 5 @15:12:42.34 Air.in :pressurize @15:12:42.39 Air.seek :pressurized @15:12:48.77 17.9psi after pressurize @15:12:48.79 Air.in :compress @15:12:48.84 Air.seek :evac @15:12:52.99 Reached 40.7 of 40.0psi after pumping 1633uL (0.92 strokes) @15:12:53.00 Air.in :default @15:12:53.05 Air.hold @15:12:53.13 delay 2 seconds @15:12:55.16 Air.in :fast @15:12:55.21 Air.seek :full @15:13:01.21 Plunge.seek :puckBottom @15:13:02.15 Lysis1.seek 138uL @15:13:08.70 delay 2 seconds @15:13:10.73 Plunge.seek :puckTop @15:13:11.60 Lysis1.seek :empty @15:13:18.15 Plunge.seek :puckBottom @15:13:19.26 Lysis1.in :extract @15:13:19.34 Lysis1.seek 675uL,nil @15:13:22.26 Air.in :pressurize @15:13:22.35 Air.seek :pressurized @15:13:22.68 Syringe::LysatePlungerStuck in PROCESSING -- at < 332uL during Lysis Syringe #1 (LyS1) move @15:13:22.71 Lysis1.in :default @15:13:22.77 Lysis1.seek 302uL @15:13:24.27 Reached 12.7 of 15.5psi after pumping 371uL (0.21 strokes) @15:13:24.29 Air.in :default @15:13:24.34 Air.hold @15:13:24.59 Air.in :pressurize @15:13:24.66 Air.seek :pressurized @15:13:29.44 12.5psi after pressurize @15:13:29.46 Air.in :compressLysate @15:13:29.51 Air.seek :evac @15:13:31.80 Reached 15.7 of 15.5psi after pumping 1437uL (0.81 strokes) @15:13:31.82 Air.in :default @15:13:31.87 Air.hold @15:13:31.99 Lysis1.in :extract @15:13:32.14 Lysis1.seek 675uL,nil @15:13:33.97 Air.in :compressLysate @15:13:34.06 Air.seek :evac @15:13:34.31 Syringe::LysatePlungerStuck in PROCESSING -- at < 437uL during Lysis Syringe #1 (LyS1) move @15:13:34.34 Lysis1.in :default @15:13:34.41 Lysis1.seek 407uL @15:13:35.90 Reached 15.5 of 15.5psi after pumping 1461uL (0.82 strokes) @15:13:35.92 Air.in :default @15:13:35.97 Air.hold @15:13:36.44 Lysis1.in :extract @15:13:36.49 Lysis1.seek 675uL,nil @15:13:37.97 Air.in :compressLysate @15:13:38.04 Air.seek :evac @15:13:38.30 Syringe::LysatePlungerStuck in PROCESSING -- at < 487uL during Lysis Syringe #1 (LyS1) move @15:13:38.33 Lysis1.in :default @15:13:38.40 Lysis1.seek 457uL @15:13:39.50 Reached 16.0 of 15.5psi after pumping 1479uL (0.83 strokes) @15:13:39.52 Air.in :default @15:13:39.56 Air.hold @15:13:39.87 Lysis1.in :extract @15:13:39.93 Lysis1.seek 675uL,nil @15:13:41.57 Air.in :compressLysate @15:13:41.66 Air.seek :evac @15:13:42.09 Syringe::LysatePlungerStuck in PROCESSING -- at < 584uL during Lysis Syringe #1 (LyS1) move @15:13:42.12 Lysis1.in :default @15:13:42.18 Lysis1.seek 554uL @15:13:44.80 Reached 15.6 of 15.5psi after pumping 1521uL (0.86 strokes) @15:13:44.82 Air.in :default @15:13:44.88 Air.hold @15:13:45.01 Lysis1.in :extract @15:13:45.11 Lysis1.seek 675uL,nil @15:13:46.97 Air.in :compressLysate @15:13:47.02 Air.seek :evac @15:13:47.25 Syringe::LysatePlungerStuck in PROCESSING -- at < 648uL during Lysis Syringe #1 (LyS1) move @15:13:47.29 Lysis1.in :default @15:13:47.34 Lysis1.seek 618uL @15:13:48.87 Reached 15.6 of 15.5psi after pumping 1545uL (0.87 strokes) @15:13:48.89 Air.in :default @15:13:48.94 Air.hold @15:13:49.40 Lysis1.in :extract @15:13:49.46 Lysis1.seek 675uL,nil @15:13:50.97 Air.in :compressLysate @15:13:51.02 Air.seek :evac @15:13:51.21 Air.in :default @15:13:51.27 Air.hold @15:13:52.06 Pulled 675uL lysate @14.5psi @15:13:52.35 Lysis1.seek 800uL,nil @15:13:52.67 Air.in :compressLysate @15:13:52.81 Air.seek :evac @15:14:02.59 Air.in :default @15:14:02.64 Air.hold @15:14:02.92 Air.in :glide @15:14:02.98 Air.seek :fullVent @15:14:05.55 Handoff.select :waste @15:14:06.04 Recovered 800uL lysate @15.0psi @15:14:06.06 delay 3 seconds @15:14:09.14 After 3 delay Recovered 800uL lysate @0.8psi @15:14:09.35 Handoff.select :close @15:14:09.86 Gate.power :heat,:OFF @15:14:09.88 Gate.power :cartridge,:OFF @15:14:09.96 PinPort.in :unlocking @15:14:10.03 PinPort.seek :clear @15:14:10.87 Carousel.in :default @15:14:10.99 Carousel.home.seek 52 @15:14:11.32 Plunge.seek :locked @15:14:12.70 Archive.in :unlocking @15:14:12.83 Archive.seek :clear @15:14:12.94 Air.in :unlocking @15:14:13.59 Air.seek :clear @15:14:13.69 Lysis1.in :unlocking @15:14:13.83 Lysis1.seek :clear @15:14:13.94 Lysis2.in :unlocking @15:14:14.03 Lysis2.seek :clear @15:14:18.85 Gate.power :motor,:OFF @15:14:18.87 Gate.power :analytic1,:OFF @15:14:18.88 Gate.power :core,:OFF @15:14:18.90 Gate.power :main,:OFF @15:14:18.91 Safely stopped and ready to power off @15:28:13.01 Session Begun @15:28:58.64 -> lysis :microSPR_phyto @15:29:27.16 -> lysis=:lyseHold_phyto :lyseHold_phyto @15:29:41.24 -> start {fire lysis} # @15:29:41.45 Gate.power :sampler,:OFF,limit to 3500mA @15:29:41.50 Gate.power :skimmer,:OFF,limit to 3500mA @15:29:41.54 Gate.power :main,:ON,limit to 5000mA @15:29:41.58 Gate.power :analytic1,:ON,limit to 4000mA @15:29:41.69 Powering core bus @15:29:44.46 Plunge.configure :default @15:29:44.57 SamplePump.configure :default @15:29:44.67 Toroid.configure :default @15:29:44.78 Carousel.configure :default @15:29:44.88 Abs.configure :default @15:29:45.00 PhaseSeparator.configure :full @15:29:45.10 Archive.configure :default @15:29:45.20 Air.configure :default @15:29:45.29 Lysis1.configure :default @15:29:45.37 Lysis2.configure :default @15:29:45.73 PinPort.configure :default @15:29:45.81 All elf controllers running firmware version 4.94 @15:29:45.85 Gate.power :motor,:ON,limit to 5000mA @15:29:48.94 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @15:29:48.97 AnalyticModule::Unknown in detached from han -- SPR @15:29:49.39 Plunge.home.jog 0 @15:29:50.07 PinPort.home.jog 0 @15:29:50.11 Lysis1.home.jog 0 @15:29:50.17 Carousel.home.jog 0 @15:29:50.25 Air.home.jog 0 @15:29:50.30 Archive.home.jog 0 @15:29:50.34 Toroid.home.jog 0 @15:29:50.38 Lysis2.home.jog 0 @15:29:50.72 -> exit @15:29:50.87 Session Ended @15:29:51.39 Plunge.in :homing @15:29:51.77 Archive.in :homing @15:29:51.86 Plunge.jog 1481 @15:29:51.89 Lysis2.in :homing @15:29:51.92 Air.in :homing @15:29:51.94 PinPort.in :homing @15:29:51.96 Toroid.in :homing @15:29:51.98 Lysis1.in :homing @15:29:52.28 Archive.jog 311 @15:29:52.37 Lysis2.jog 1617 @15:29:52.48 Air.jog 1427 @15:29:52.62 PinPort.jog 1228 @15:29:52.65 Lysis1.jog 1598 @15:29:52.73 Toroid.jog 1365 @15:29:53.09 Archive.setRawPosition! 50 @15:29:53.15 Archive.seek :home @15:29:53.37 Plunge.setRawPosition! 50 @15:29:53.44 Plunge.seek :home @15:29:53.53 Archive.in :default @15:29:53.71 Plunge.in :default @15:29:53.81 Lysis2.setRawPosition! 50 @15:29:53.86 Lysis2.seek :home @15:29:53.99 Air.setRawPosition! 0 @15:29:54.51 Air.seek :home @15:29:54.74 PinPort.setRawPosition! 3150 @15:29:54.82 Air.in :default @15:29:54.87 Lysis2.in :default @15:29:54.93 Lysis1.setRawPosition! 50 @15:29:54.97 PinPort.seek :home @15:29:55.13 Lysis1.seek :home @15:29:55.46 Lysis1.in :default @15:29:57.48 Toroid.setRawPosition! 0 @15:29:57.54 Toroid.seek :home @15:29:57.64 Toroid.in :default @15:29:59.52 PinPort.in :default @15:29:59.90 Toroid.seek :clear @15:30:00.11 PinPort.in :unlocking @15:30:00.16 PinPort.seek :clear @15:30:03.55 Air.seek :clear @15:30:03.64 Lysis1.seek :clear @15:30:04.37 Lysis2.seek :clear @15:30:05.15 Archive.seek :clear @15:30:05.93 Carousel.in :default @15:30:06.09 Carousel.home.seek 52 @15:30:06.37 Selecting Cartridge 22 @15:30:06.67 Carousel.seek 22 @15:30:13.13 Carousel.reconfigure :align @15:30:13.25 Lysis1.in :fast @15:30:13.30 Lysis1.seek :locked @15:30:15.92 Plunge.seek :locked @15:30:16.14 Air.in :fast @15:30:16.20 Air.seek :locked @15:30:17.27 Carousel.reconfigure :locked @15:30:17.42 Powering cartridge bus @15:30:20.09 Gate.setIR 1750,5000,0,6 @15:30:20.21 Gate.setIR 0,5000,0,6 @15:30:20.28 PuckHeater.configure :default #ambient 23.0C @15:30:20.34 Gate.power :heat,:ON,limit to 5000mA @15:30:20.51 Mounted lysis Cartridge board#J60003 @15:30:20.55 Using default cartridge heat loss curve for HeatingFunctionInCarousel @15:30:20.87 Preparing to filter lyseHold_phyto cartridge in slot 22 @15:30:21.14 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @15:30:27.77 Intake Head Pressure tare is 0% of full scale at 23C (drifted 1%) @15:30:27.79 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @15:30:27.81 PhaseSeparator.in :filling @15:30:27.87 PhaseSeparator.hold @15:30:27.95 Gate.power :sampler,:ON @15:30:34.99 Head pressure reached 3.52psi in 8 seconds @15:30:35.02 Air.in :default @15:30:35.14 Air.seek :sealed @15:30:35.20 Priming sample loop w/25ml, bypass w/10ml @15:30:35.23 SamplePump.setPosition! 0ml @15:30:35.32 Bypass.open @15:30:37.64 Intake.open @15:30:39.25 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:30:41.87 Vacuum tare is 53% of full scale at 23C (drifted 2%) @15:30:42.06 Abs.in :default @15:30:42.12 Abs.hold @15:30:42.19 PhaseSeparator.in :full @15:30:42.24 PhaseSeparator.hold @15:30:42.47 Waiting for sampler to finish priming... @15:30:42.49 delayUntil "15:31:27.97" @15:31:28.08 SamplePump.in :start @15:31:28.14 SamplePump.seek 10ml,1:10 @15:31:28.78 SamplePump.configure :prime @15:31:41.67 Abs.stop @15:31:41.72 PhaseSeparator.stop @15:31:41.78 SamplePump.setPosition! 0ml @15:31:41.83 Exhaust.open @15:31:44.05 Bypass.close @15:31:46.44 Abs.in :default @15:31:46.50 Abs.hold @15:31:46.57 PhaseSeparator.hold @15:31:46.90 SamplePump.in :start @15:31:46.96 SamplePump.seek 25ml,2:10 @15:31:47.60 SamplePump.configure :prime @15:32:20.64 Abs.stop @15:32:20.73 PhaseSeparator.stop @15:32:20.81 Bypass.open @15:32:22.34 Intake.close @15:32:23.97 Toroid.in :hold @15:32:24.05 Toroid.seek :crack @15:32:26.54 Toroid.seek :cartridge @15:32:28.66 Toroid.in :default @15:32:28.72 Toroid.seek :clear @15:32:32.69 Pressurizing cartridge to 14psi above 14.2psia @15:32:32.71 SamplePump.setPosition! 0ml @15:32:32.76 Intake.open @15:32:34.30 Bypass.close @15:32:35.84 Exhaust.close @15:32:37.51 Abs.in :default @15:32:37.56 Abs.hold @15:32:37.64 PhaseSeparator.hold @15:32:37.89 SamplePump.in :charge @15:32:37.95 SamplePump.seek 2.3ml,38 seconds @15:32:53.44 Abs.stop @15:32:53.50 PhaseSeparator.stop @15:32:53.56 Intake.close @15:32:55.15 Pressurized cartridge with 0.998ml to 14.0psi @15:32:55.17 Isolated @28.3psia (14.0psi) @15:32:55.20 Verifying cartridge holds at least 0.65..0.85 of 28.3psia @15:32:55.23 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:32:59.82 Cartridge pressure fell 1% to 28.0psia @15:32:59.93 Sampling 100.0ml @15:32:59.94 SamplePump.setPosition! 0ml @15:33:00.02 Exhaust.open @15:33:01.52 Intake.open @15:33:03.20 Abs.in :default @15:33:03.25 Abs.hold @15:33:03.33 PhaseSeparator.hold @15:33:03.67 SamplePump.in :start @15:33:04.32 SamplePump.seek 100ml,6:04 @15:33:04.39 SamplePump.configure :sample @15:33:06.76 Cartridge 22 filtering 1/100ml at 0.57ml/s with 13.7psi vacuum @15:34:06.84 Cartridge 22 filtering 42/100ml at 0.67ml/s with 13.9psi vacuum @15:35:06.81 Cartridge 22 filtering 82/100ml at 0.68ml/s with 10.5psi vacuum @15:35:32.85 Abs.stop @15:35:32.90 PhaseSeparator.stop @15:35:32.99 Intake.close @15:35:33.04 Exhaust.close @15:35:33.31 Toroid.in :hold @15:35:33.36 Toroid.seek :bypass @15:35:36.95 Toroid.in :default @15:35:37.01 Toroid.seek :clear @15:35:40.59 Sampled 100.0ml @15:35:41.13 Handoff.select :waste @15:35:41.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:35:43.73 Gate.power :sampler,:OFF @15:35:48.25 Syringe tare is 51% of full scale at 23C (drifted -0%) @15:35:48.28 PinPort.in :locking @15:35:48.33 PinPort.seek :locked @15:35:49.17 Archive.in :fast @15:35:49.26 Lysis2.in :fast @15:35:49.31 Lysis1.seek 1250uL @15:35:49.37 Archive.seek :empty @15:35:49.49 Lysis2.seek :empty @15:35:57.98 Evacuating puck with filter bubble point of 20psi @15:35:58.04 PinPort.seek :locked @15:35:58.14 Handoff.select :waste @15:35:58.16 Plunge.seek :puckBottom @15:35:59.47 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:36:06.20 Air syringe pumping up to 1 strokes to reach 4psi @15:36:06.21 Air stroke #1 of 1 @15:36:06.31 Air.in :pressurize @15:36:06.37 Air.seek :pressurized @15:36:11.61 0.4psi after pressurize @15:36:11.63 Air.in :compress @15:36:11.68 Air.seek :evac @15:36:18.29 3.0psi after compress @15:36:18.51 Reached 3.0 of 4.0psi after pumping 1775uL (1.00 strokes) @15:36:18.52 Air.in :default @15:36:18.57 Air.hold @15:36:18.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:36:30.24 Stabilized at -0.0psi after evac to waste @15:36:30.26 Handoff.select :blocked @15:36:30.75 Plunge.seek :puckTop @15:36:31.63 Air syringe pumping up to 8 strokes to reach 20psi @15:36:31.64 Air stroke #1 of 8 @15:36:31.73 Air.in :compress @15:36:31.78 Air.seek :evac @15:36:31.93 1.1psi after compress @15:36:42.51 1.3psi after compress (stable) @15:36:42.54 Air.in :gulp @15:36:43.01 Air.seek :topVent @15:36:49.59 1.2psi after gulp @15:36:55.18 1.5psi after gulp (stable) @15:36:55.19 Air stroke #2 of 8 @15:36:55.28 Air.in :pressurize @15:36:55.34 Air.seek :pressurized @15:37:01.71 1.3psi after pressurize @15:37:01.73 Air.in :compress @15:37:01.78 Air.seek :evac @15:37:08.39 12.7psi after compress @15:37:19.98 15.6psi after compress (stable) @15:37:20.03 Air.in :gulp @15:37:20.10 Air.seek :topVent @15:37:25.72 Reached 15.5 of 20.0psi after pumping 3551uL (2.00 strokes) @15:37:25.74 Air.in :default @15:37:25.79 Air.hold @15:37:25.87 Plunge.seek :puckBottom @15:37:26.74 delay 3 seconds @15:37:29.76 Handoff.select :waste @15:37:30.24 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:37:40.33 delay 1:00 @15:38:40.44 Handoff.select :blocked @15:38:40.93 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @15:39:03.52 Handoff.select :waste @15:39:04.03 -0.1psi at evacEnd @15:39:04.06 Plunge.seek :pTop @15:39:04.92 delay 5 seconds @15:39:09.95 Air.in :glide @15:39:10.03 Air.seek :fullVent @15:39:25.27 delay 2 seconds @15:39:27.29 Plunge.seek :pBottom @15:39:28.15 Seawater evacuated, Pressure above/below filter released. @15:39:28.17 Plunge.seek :locked @15:39:29.46 Handoff.select :waste @15:39:29.56 Lysis1.in :slow @15:39:30.18 Lysis1.seek 950uL @15:39:44.25 delay 5 seconds @15:39:49.28 Handoff.select :blocked @15:39:49.80 Plunge.seek :puckTop @15:39:50.77 Lysis1.seek 350uL @15:40:18.76 delay 3 seconds @15:40:21.86 Air.in mix with 2.79934 parts Lysis1 @15:40:22.52 Air.seek :evac @15:40:22.60 Lysis1.seek 190uL @15:40:30.23 Air.in :fast @15:40:30.28 Air.seek :full @15:40:36.79 Lysis1.seek :empty @15:40:45.89 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @23.7psi @15:40:45.91 Plunge.seek :puckBottom @15:40:46.90 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @20.2psi @15:40:46.92 Polling Can every 2:00 @15:40:47.47 PuckHeater.configure :default #ambient 23.2C @15:40:47.53 PuckHeater.seek 81.6C @15:42:04.21 PuckHeater.hold 2:00,81.6C @15:44:04.14 Polling Can every 2:00 @15:44:04.68 PuckHeater.configure :default #ambient 23.2C @15:44:04.74 PuckHeater.seek 80.6C @15:44:04.94 PuckHeater.hold 2:00,80.6C @15:46:04.85 Polling Can every 2:00 @15:46:05.39 PuckHeater.configure :default #ambient 23.2C @15:46:05.45 PuckHeater.hold 8:00,79.5C @15:54:04.75 Lysate cooked @24.7psi @15:54:04.77 Plunge.seek :puckTop @15:54:05.64 Air syringe pumping up to 5 strokes to reach 40psi @15:54:05.65 Air stroke #1 of 5 @15:54:05.74 Air.in :pressurize @15:54:05.79 Air.seek :pressurized @15:54:12.17 23.2psi after pressurize @15:54:12.19 Air.in :compress @15:54:12.24 Air.seek :evac @15:54:17.30 Reached 41.1 of 40.0psi after pumping 1685uL (0.95 strokes) @15:54:17.32 Air.in :default @15:54:17.37 Air.hold @15:54:17.45 delay 2 seconds @15:54:19.48 Air.in :fast @15:54:19.53 Air.seek :full @15:54:25.71 Plunge.seek :puckBottom @15:54:26.65 Lysis1.seek 138uL @15:54:33.20 delay 2 seconds @15:54:35.23 Plunge.seek :puckTop @15:54:36.09 Lysis1.seek :empty @15:54:42.65 Plunge.seek :puckBottom @15:54:43.74 Lysis1.in :extract @15:54:43.84 Lysis1.seek 675uL,nil @15:54:47.19 Air.in :pressurize @15:54:47.27 Air.seek :pressurized @15:54:53.74 10.1psi after pressurize @15:54:53.76 Air.in :compressLysate @15:54:53.81 Air.seek :evac @15:55:09.70 Syringe::LysatePlungerStuck in PROCESSING -- at < 568uL during Lysis Syringe #1 (LyS1) move @15:55:09.73 Lysis1.in :default @15:55:09.79 Lysis1.seek 538uL @15:55:10.92 Reached 16.0 of 15.5psi after pumping 1648uL (0.93 strokes) @15:55:10.94 Air.in :default @15:55:10.99 Air.hold @15:55:11.30 Lysis1.in :extract @15:55:11.35 Lysis1.seek 675uL,nil @15:55:12.96 Air.in :compressLysate @15:55:13.04 Air.seek :evac @15:55:13.18 Air.in :default @15:55:13.24 Air.hold @15:55:13.50 Pulled 675uL lysate @15.9psi @15:55:13.68 Lysis1.seek 800uL,nil @15:55:13.93 Air.in :compressLysate @15:55:14.00 Air.seek :evac @15:55:20.70 Reached 14.9 of 15.5psi after pumping 1742uL (0.98 strokes) @15:55:20.73 Air.in :default @15:55:20.81 Air.hold @15:55:21.17 Air.in :glide @15:55:21.22 Air.seek :fullVent @15:55:23.26 Handoff.select :waste @15:55:23.75 Recovered 800uL lysate @16.1psi @15:55:23.77 delay 3 seconds @15:55:26.84 After 3 delay Recovered 800uL lysate @1.9psi @15:55:27.06 Handoff.select :close @15:55:27.57 Gate.power :heat,:OFF @15:55:27.59 Gate.power :cartridge,:OFF @15:55:27.67 PinPort.in :unlocking @15:55:27.72 PinPort.seek :clear @15:55:28.55 Carousel.in :default @15:55:28.67 Carousel.home.seek 22 @15:55:29.01 Plunge.seek :locked @15:55:30.40 Archive.in :unlocking @15:55:30.53 Archive.seek :clear @15:55:30.62 Air.in :unlocking @15:55:30.77 Air.seek :clear @15:55:30.86 Lysis1.in :unlocking @15:55:31.04 Lysis1.seek :clear @15:55:31.13 Lysis2.in :unlocking @15:55:31.22 Lysis2.seek :clear @15:55:36.05 Gate.power :motor,:OFF @15:55:36.07 Gate.power :analytic1,:OFF @15:55:36.08 Gate.power :core,:OFF @15:55:36.09 Gate.power :main,:OFF @15:55:36.11 Safely stopped and ready to power off nohup: ESP: No such file or directory @17:37:41.46EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @17:37:45.24 >BREAK< ESP3 Gateway v1.44 for core bus @17:37:45.31 >BREAK< ESP3 Gateway v1.44 for cartridge bus @17:37:49.44 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @17:37:50.11 Sea pressure (Abs) tare is 6% of full scale @17:37:53.05 Polling Can every 2:00 @17:37:53.10 Waiting for espclients... @17:37:54.03 Session Begun @17:41:28.58 -> exit @17:41:28.61 Session Ended @17:41:34.36 Session Begun @17:41:40.05 -> MainThread.exit # @17:41:40.16 ThreadError in preston -- killed thread @17:41:40.23 Session Ended @17:50:36.78EDT29-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @17:50:39.20 >BREAK< ESP3 Gateway v1.44 for core bus @17:50:39.23 >BREAK< ESP3 Gateway v1.44 for cartridge bus @17:50:42.92 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @17:50:43.66 Sea pressure (Abs) tare is 6% of full scale @17:50:46.69 Polling Can every 2:00 @17:50:46.75 Waiting for espclients... @17:50:47.74 Session Begun @17:55:56.07 -> slots {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processing, lysed: 1525, lysed2: 1525, spr: "spr26jul2907h.spr.bz2"}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2906h.spr.bz2"}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2905h.spr.bz2"}, [52,22] => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2903h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2902h.spr.bz2"}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2901h.spr.bz2"}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2823h.spr.bz2"}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2822h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2821h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 1525, lysed2: 1525, spr: "spr26jul2819h.spr.bz2"}} @17:58:04.93 -> slots [60,54,48,42,36,30,24,17,12,6]=>nil {[60,54,48,42,36,30,24,17,12,6] => nil} @17:58:09.26 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, [52,22] => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @17:59:30.71 -> slots [60,54,48,42,36,30,24,17,12,6]=>[:microSPR_phyto, :standard, {lysed:800, lysed2:800}] {[60,54,48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 800, lysed2: 800}} @17:59:37.85 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, [60,54,48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 800, lysed2: 800}, [52,22] => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @18:00:12.59 -> Cart.aspectsOf :standard, extraDilutions:[1], lysed:800, lysed2:800 {extraDilutions: [1], lysed: 800, lysed2: 800} @18:00:40.02 -> Cart.aspect {standard: {extraDilutions: [1], lysed: 800, lysed2: 800}} @18:00:54.79 -> ESP.shutdown @18:00:54.84 Gate.power :motor,:OFF @18:00:54.87 Gate.power :analytic1,:OFF @18:00:54.91 Gate.power :core,:OFF @18:00:54.95 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @18:13:33.71 -> ESP.ready! @18:13:33.76 Gate.power :sampler,:OFF,limit to 3500mA @18:13:33.79 Gate.power :skimmer,:OFF,limit to 3500mA @18:13:33.84 Gate.power :main,:ON,limit to 5000mA @18:13:33.87 Gate.power :analytic1,:ON,limit to 4000mA @18:13:34.06 Powering core bus @18:13:36.32 SPR Module 1, FW Rev. B, FPGA Rev. C @18:13:36.84 Plunge.configure :default @18:13:36.96 SamplePump.configure :default @18:13:37.06 Toroid.configure :default @18:13:37.22 Carousel.configure :default @18:13:37.33 Abs.configure :default @18:13:37.44 PhaseSeparator.configure :default @18:13:37.55 Archive.configure :default @18:13:37.66 Air.configure :default @18:13:37.74 Lysis1.configure :default @18:13:37.81 Lysis2.configure :default @18:13:37.92 PinPort.configure :default @18:13:38.01 All elf controllers running firmware version 4.94 @18:13:38.04 Gate.power :motor,:ON,limit to 5000mA @18:13:38.40 Plunge.home.jog 0 @18:13:39.13 PinPort.home.jog 0 @18:13:39.19 Lysis2.home.jog 0 @18:13:39.23 Air.home.jog 0 @18:13:39.28 Carousel.home.jog 0 @18:13:39.39 Archive.home.jog 0 @18:13:39.41 Toroid.home.jog 0 @18:13:39.45 Lysis1.home.jog 0 @18:13:40.35 Plunge.in :homing @18:13:40.59 Plunge.jog 1490 @18:13:40.67 Lysis2.in :homing @18:13:40.72 Air.in :homing @18:13:40.76 Toroid.in :homing @18:13:40.81 Lysis1.in :homing @18:13:40.87 PinPort.in :homing @18:13:40.92 Archive.in :homing @18:13:41.29 Lysis2.jog 1621 @18:13:41.36 Air.jog 1428 @18:13:41.42 Lysis1.jog 1598 @18:13:41.47 PinPort.jog 1229 @18:13:41.51 Toroid.jog 1360 @18:13:41.55 Archive.jog 301 @18:13:42.23 Plunge.setRawPosition! 50 @18:13:42.31 Plunge.seek :home @18:13:42.45 Archive.setRawPosition! 50 @18:13:42.52 Archive.seek :home @18:13:42.66 Plunge.in :default @18:13:42.82 Archive.in :default @18:13:42.96 Lysis2.setRawPosition! 50 @18:13:42.99 Air.setRawPosition! 0 @18:13:43.18 Lysis2.seek :home @18:13:43.21 Air.seek :home @18:13:43.45 Lysis1.setRawPosition! 50 @18:13:43.47 Air.in :default @18:13:43.62 Lysis1.seek :home @18:13:44.30 PinPort.setRawPosition! 3150 @18:13:44.31 Lysis1.in :default @18:13:44.36 Lysis2.in :default @18:13:44.52 PinPort.seek :home @18:13:46.28 Toroid.setRawPosition! 0 @18:13:46.35 Toroid.seek :home @18:13:46.47 Toroid.in :default @18:13:48.97 PinPort.in :default @18:13:49.33 Toroid.seek :clear @18:13:49.56 PinPort.in :unlocking @18:13:49.65 PinPort.seek :clear @18:13:53.06 Air.seek :clear @18:13:53.23 Lysis1.seek :clear @18:13:53.98 Lysis2.seek :clear @18:13:54.79 Archive.seek :clear @18:13:55.60 Carousel.in :default @18:13:55.74 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @3.77psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:14:48.39 -> slots {1 => { Type: :archiveHiBiomass_bac, State: :missing}, [60,54,48,42,36,30,24,17,12,6] => { Type: :microSPR_phyto, State: :standard, lysed: 800, lysed2: 800}, [52,22] => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @18:15:11.19 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 800, lysed2: 800}} @18:16:07.85 -> start {fireAll "standard"} # @18:16:08.34 Cartridge group: standard @18:16:09.23 Toroid.seek :clear @18:16:09.47 Air.seek :clear @18:16:09.60 Lysis1.seek :clear @18:16:09.73 Lysis2.seek :clear @18:16:09.86 Archive.seek :clear @18:16:09.97 Carousel.in :default @18:16:10.13 Carousel.home.seek 22 @18:16:10.46 Selecting Cartridge 60 @18:16:10.81 Carousel.seek 60 @18:16:18.92 Carousel.reconfigure :align @18:16:19.52 Lysis1.in :fast @18:16:19.72 Lysis1.seek :locked @18:16:22.38 Plunge.seek :locked @18:16:22.58 Air.in :fast @18:16:22.68 Air.seek :locked @18:16:23.78 Carousel.reconfigure :locked @18:16:23.99 Powering cartridge bus @18:16:26.66 Gate.setIR 1750,5000,0,6 @18:16:26.79 Gate.setIR 0,5000,0,6 @18:16:26.88 PuckHeater.configure :default @18:16:26.99 Gate.power :heat,:ON,limit to 5000mA @18:16:27.30 Mounted lysis Cartridge board#J60008 @18:16:27.37 Using default cartridge heat loss curve for HeatingFunctionInCarousel @18:16:28.98 SPR.command "read_sequences" @18:16:29.30 SPR.command "stats_on" @18:16:29.50 SPR.command "start 0 1" @18:16:30.19 SPR.start /var/log/esp/spr26jul2918h.spr.bz2 @18:16:32.14 Waiting for baseline SPR stabilization @18:16:32.18 Handoff.select :waste @18:16:32.73 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:16:39.37 Syringe tare is 51% of full scale at 23C (drifted -0%) @18:16:39.41 PinPort.in :locking @18:16:39.51 PinPort.seek :locked @18:16:40.40 Archive.in :fast @18:16:40.47 Lysis2.in :fast @18:16:40.60 Lysis1.seek 800uL @18:16:40.74 Archive.seek :empty @18:16:40.92 Lysis2.seek 800uL @18:16:49.19 delayUntil "18:26:31.69" @18:17:01.76 -> exit @18:17:01.79 Session Ended @18:26:31.70 Lysis2.in :DAmix @18:26:31.83 Lysis1.in :DAmix @18:26:32.34 Lysis2.seek 550uL @18:26:32.43 Lysis1.seek 550uL @18:27:07.41 delay 5 seconds @18:27:12.43 Handoff.select SPR @18:27:12.91 SPR.command "start 2 14" @18:27:14.37 SPR stabilized @18:27:14.52 delay 20 seconds @18:27:34.55 SPR.command "start 2 0" @18:27:37.21 Waiting up to 11:00 for SPR to enter state 1 @18:29:34.85 SPR.command "hold_on" @18:29:36.26 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:29:36.89 Lysis2.seek 250uL @18:29:36.97 Lysis1.seek 250uL @18:30:18.90 delay 15 seconds @18:30:33.92 SPR.command "hold_off" @18:30:36.91 Waiting up to 1:00:00 for SPR to enter state 7 @18:46:52.48 SPRsummary:5.76RIU,197RIU,184RIU,314RIU after 1924s @19:01:09.53 delay 20 seconds @19:01:29.55 SPR.command "start 2 0" @19:01:32.64 Waiting up to 11:00 for SPR to enter state 1 @19:03:30.30 SPR.command "hold_on" @19:03:31.68 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:03:32.35 Lysis2.seek 100uL @19:03:32.41 Lysis1.seek 100uL @19:03:53.47 delay 15 seconds @19:04:08.50 SPR.command "hold_off" @19:04:12.06 Waiting up to 1:00:00 for SPR to enter state 7 @19:34:44.95 SPR analysis completed @19:34:45.55 Ended log spr26jul2918h.spr.bz2 @19:34:45.57 SPR.command "stats_off" @19:34:46.89 Handoff.select :close @19:34:47.41 Gate.power :heat,:OFF @19:34:47.43 Gate.power :cartridge,:OFF @19:34:47.51 PinPort.in :unlocking @19:34:47.57 PinPort.seek :clear @19:34:48.39 Carousel.in :default @19:34:48.52 Carousel.home.seek 60 @19:34:48.85 Plunge.seek :locked @19:34:49.04 Archive.in :unlocking @19:34:49.20 Archive.seek :clear @19:34:49.30 Air.in :unlocking @19:34:49.46 Air.seek :clear @19:34:49.59 Lysis1.in :unlocking @19:34:49.73 Lysis1.seek :clear @19:34:49.78 Lysis2.in :unlocking @19:34:49.90 Lysis2.seek :clear @19:34:54.51 Gate.power :motor,:OFF @19:34:54.53 Gate.power :analytic1,:OFF @19:34:54.54 Gate.power :core,:OFF @19:34:54.56 Gate.power :main,:OFF @19:34:54.57 Safely stopped and ready to power off @19:34:55.28 Gate.power :sampler,:OFF,limit to 3500mA @19:34:55.29 Gate.power :skimmer,:OFF,limit to 3500mA @19:34:58.37 Gate.power :main,:ON,limit to 5000mA @19:34:58.38 Gate.power :analytic1,:ON,limit to 4000mA @19:34:58.45 Powering core bus @19:35:00.81 SPR Module 1, FW Rev. B, FPGA Rev. C @19:35:01.27 Plunge.configure :default @19:35:01.35 SamplePump.configure :default @19:35:01.44 Toroid.configure :default @19:35:01.52 Carousel.configure :default @19:35:01.60 Abs.configure :default @19:35:01.69 PhaseSeparator.configure :default @19:35:01.77 Archive.configure :default @19:35:02.39 Air.configure :default @19:35:02.45 Lysis1.configure :default @19:35:02.51 Lysis2.configure :default @19:35:02.59 PinPort.configure :default @19:35:02.65 All elf controllers running firmware version 4.94 @19:35:02.67 Gate.power :motor,:ON,limit to 5000mA @19:35:02.91 Plunge.home.jog 0 @19:35:03.73 Lysis2.home.jog 0 @19:35:03.78 Air.home.jog 0 @19:35:03.81 Carousel.home.jog 0 @19:35:03.84 Lysis1.home.jog 0 @19:35:03.88 Archive.home.jog 0 @19:35:03.91 Toroid.home.jog 0 @19:35:03.95 PinPort.home.jog 0 @19:35:04.88 Plunge.in :homing @19:35:05.22 Plunge.jog 1489 @19:35:05.30 Lysis2.in :homing @19:35:05.34 Air.in :homing @19:35:05.36 Toroid.in :homing @19:35:05.38 PinPort.in :homing @19:35:05.40 Archive.in :homing @19:35:05.43 Lysis1.in :homing @19:35:05.74 Lysis2.jog 1618 @19:35:05.77 Air.jog 1431 @19:35:05.84 Toroid.jog 1362 @19:35:05.93 PinPort.jog 1239 @19:35:06.06 Archive.jog 304 @19:35:06.12 Lysis1.jog 1602 @19:35:06.69 Plunge.setRawPosition! 50 @19:35:06.74 Plunge.seek :home @19:35:06.84 Archive.setRawPosition! 50 @19:35:06.90 Archive.seek :home @19:35:07.02 Plunge.in :default @19:35:07.20 Archive.in :default @19:35:07.29 Lysis2.setRawPosition! 50 @19:35:07.32 Air.setRawPosition! 0 @19:35:07.41 Lysis2.seek :home @19:35:07.45 Air.seek :home @19:35:07.65 Air.in :default @19:35:07.68 Lysis1.setRawPosition! 50 @19:35:07.79 Lysis1.seek :home @19:35:07.92 Lysis2.in :default @19:35:07.99 PinPort.setRawPosition! 3150 @19:35:08.05 PinPort.seek :home @19:35:08.15 Lysis1.in :default @19:35:10.73 Toroid.setRawPosition! 0 @19:35:10.79 Toroid.seek :home @19:35:10.88 Toroid.in :default @19:35:12.42 PinPort.in :default @19:35:12.76 Toroid.seek :clear @19:35:12.94 PinPort.in :unlocking @19:35:12.99 PinPort.seek :clear @19:35:16.40 Air.seek :clear @19:35:16.50 Lysis1.seek :clear @19:35:17.22 Lysis2.seek :clear @19:35:18.00 Archive.seek :clear @19:35:18.78 Carousel.in :default @19:35:19.34 Carousel.home.seek 60 @19:35:19.72 Selecting Cartridge 54 @19:35:20.02 Carousel.seek 54 @19:35:21.61 Carousel.reconfigure :align @19:35:21.74 Lysis1.in :fast @19:35:21.79 Lysis1.seek :locked @19:35:24.41 Plunge.seek :locked @19:35:24.59 Air.in :fast @19:35:24.64 Air.seek :locked @19:35:25.71 Carousel.reconfigure :locked @19:35:25.87 Powering cartridge bus @19:35:28.55 Gate.setIR 1750,5000,0,6 @19:35:28.67 Gate.setIR 0,5000,0,6 @19:35:28.75 PuckHeater.configure :default @19:35:28.81 Gate.power :heat,:ON,limit to 5000mA @19:35:28.99 Mounted lysis Cartridge board#J59921 @19:35:29.02 Using default cartridge heat loss curve for HeatingFunctionInCarousel @19:35:30.60 SPR.command "read_sequences" @19:35:30.98 SPR.command "stats_on" @19:35:31.17 SPR.command "start 0 1" @19:35:31.84 SPR.start /var/log/esp/spr26jul2919h.spr.bz2 @19:35:33.83 Waiting for baseline SPR stabilization @19:35:33.85 Handoff.select :waste @19:35:34.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:35:41.02 Syringe tare is 51% of full scale at 23C (drifted -0%) @19:35:41.05 PinPort.in :locking @19:35:41.10 PinPort.seek :locked @19:35:41.97 Archive.in :fast @19:35:42.03 Lysis2.in :fast @19:35:42.10 Lysis1.seek 800uL @19:35:42.22 Archive.seek :empty @19:35:42.34 Lysis2.seek 800uL @19:35:50.65 delayUntil "19:45:33.40" @19:45:02.65 Session Begun @19:45:06.19 -> showlog @19:45:33.42 Lysis2.in :DAmix @19:45:33.57 Lysis1.in :DAmix @19:45:34.15 Lysis2.seek 550uL @19:45:34.26 Lysis1.seek 550uL @19:46:09.23 delay 5 seconds @19:46:14.25 Handoff.select SPR @19:46:14.78 SPR.command "start 2 14" @19:46:16.59 SPR stabilized @19:46:16.80 delay 20 seconds @19:46:36.82 SPR.command "start 2 0" @19:46:39.85 Waiting up to 11:00 for SPR to enter state 1 @19:48:37.50 SPR.command "hold_on" @19:48:39.41 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:48:40.06 Lysis2.seek 250uL @19:48:40.11 Lysis1.seek 250uL @19:49:22.10 delay 15 seconds @19:49:37.14 SPR.command "hold_off" @19:49:41.01 Waiting up to 1:00:00 for SPR to enter state 7 @20:05:56.05 SPRsummary:5.66RIU,233RIU,242RIU,358RIU after 1926s @20:08:26.71 Session Ended @20:20:13.10 delay 20 seconds @20:20:33.15 SPR.command "start 2 0" @20:20:36.21 Waiting up to 11:00 for SPR to enter state 1 @20:22:33.88 SPR.command "hold_on" @20:22:35.80 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:22:36.46 Lysis2.seek 100uL @20:22:36.50 Lysis1.seek 100uL @20:22:57.59 delay 15 seconds @20:23:12.63 SPR.command "hold_off" @20:23:16.05 Waiting up to 1:00:00 for SPR to enter state 7 @20:53:47.58 SPR analysis completed @20:53:48.13 Ended log spr26jul2919h.spr.bz2 @20:53:48.15 SPR.command "stats_off" @20:53:49.56 Handoff.select :close @20:53:50.09 Gate.power :heat,:OFF @20:53:50.11 Gate.power :cartridge,:OFF @20:53:50.19 PinPort.in :unlocking @20:53:50.24 PinPort.seek :clear @20:53:51.07 Carousel.in :default @20:53:51.24 Carousel.home.seek 54 @20:53:51.58 Plunge.seek :locked @20:53:51.76 Archive.in :unlocking @20:53:51.87 Archive.seek :clear @20:53:51.98 Air.in :unlocking @20:53:52.13 Air.seek :clear @20:53:52.24 Lysis1.in :unlocking @20:53:52.40 Lysis1.seek :clear @20:53:52.46 Lysis2.in :unlocking @20:53:52.58 Lysis2.seek :clear @20:53:57.24 Gate.power :motor,:OFF @20:53:57.25 Gate.power :analytic1,:OFF @20:53:57.26 Gate.power :core,:OFF @20:53:57.28 Gate.power :main,:OFF @20:53:57.29 Safely stopped and ready to power off @20:53:57.86 Gate.power :sampler,:OFF,limit to 3500mA @20:53:57.87 Gate.power :skimmer,:OFF,limit to 3500mA @20:54:01.09 Gate.power :main,:ON,limit to 5000mA @20:54:01.10 Gate.power :analytic1,:ON,limit to 4000mA @20:54:01.21 Powering core bus @20:54:03.57 SPR Module 1, FW Rev. B, FPGA Rev. C @20:54:04.01 Plunge.configure :default @20:54:04.10 SamplePump.configure :default @20:54:04.18 Toroid.configure :default @20:54:04.26 Carousel.configure :default @20:54:04.35 Abs.configure :default @20:54:04.43 PhaseSeparator.configure :default @20:54:04.51 Archive.configure :default @20:54:04.59 Air.configure :default @20:54:04.65 Lysis1.configure :default @20:54:05.25 Lysis2.configure :default @20:54:05.38 PinPort.configure :default @20:54:05.44 All elf controllers running firmware version 4.94 @20:54:05.46 Gate.power :motor,:ON,limit to 5000mA @20:54:05.81 Plunge.home.jog 0 @20:54:06.46 Lysis2.home.jog 0 @20:54:06.54 Air.home.jog 0 @20:54:06.57 Carousel.home.jog 0 @20:54:06.61 Archive.home.jog 0 @20:54:06.63 Toroid.home.jog 0 @20:54:06.67 PinPort.home.jog 0 @20:54:06.71 Lysis1.home.jog 0 @20:54:07.67 Plunge.in :homing @20:54:07.95 Plunge.jog 1479 @20:54:08.02 Lysis2.in :homing @20:54:08.06 Air.in :homing @20:54:08.08 Toroid.in :homing @20:54:08.10 PinPort.in :homing @20:54:08.13 Archive.in :homing @20:54:08.17 Lysis1.in :homing @20:54:08.43 Lysis2.jog 1620 @20:54:08.48 Air.jog 1431 @20:54:08.54 Toroid.jog 1360 @20:54:08.71 PinPort.jog 1256 @20:54:08.78 Lysis1.jog 1603 @20:54:08.87 Archive.jog 307 @20:54:09.40 Plunge.setRawPosition! 50 @20:54:09.45 Plunge.seek :home @20:54:09.59 Archive.setRawPosition! 50 @20:54:09.64 Archive.seek :home @20:54:09.75 Plunge.in :default @20:54:09.90 Air.setRawPosition! 0 @20:54:10.01 Lysis2.setRawPosition! 50 @20:54:10.03 Archive.in :default @20:54:10.06 Air.seek :home @20:54:10.18 Lysis2.seek :home @20:54:10.40 Lysis1.setRawPosition! 50 @20:54:10.43 Air.in :default @20:54:10.51 Lysis1.seek :home @20:54:10.64 Lysis2.in :default @20:54:10.73 PinPort.setRawPosition! 3150 @20:54:10.80 PinPort.seek :home @20:54:10.90 Lysis1.in :default @20:54:13.36 Toroid.setRawPosition! 0 @20:54:13.42 Toroid.seek :home @20:54:13.50 Toroid.in :default @20:54:15.19 PinPort.in :default @20:54:15.52 Toroid.seek :clear @20:54:15.67 PinPort.in :unlocking @20:54:15.73 PinPort.seek :clear @20:54:19.15 Air.seek :clear @20:54:19.26 Lysis1.seek :clear @20:54:19.98 Lysis2.seek :clear @20:54:20.76 Archive.seek :clear @20:54:21.54 Carousel.in :default @20:54:21.66 Carousel.home.seek 54 @20:54:22.33 Selecting Cartridge 48 @20:54:22.65 Carousel.seek 48 @20:54:24.22 Carousel.reconfigure :align @20:54:24.34 Lysis1.in :fast @20:54:24.40 Lysis1.seek :locked @20:54:24.48 Slide::Warning in core -- Storage Carousel (SC) reseek at 48 + 4 counts @20:54:27.03 Plunge.seek :locked @20:54:27.24 Air.in :fast @20:54:27.30 Air.seek :locked @20:54:28.37 Carousel.reconfigure :locked @20:54:28.53 Powering cartridge bus @20:54:31.20 Gate.setIR 1750,5000,0,6 @20:54:31.33 Gate.setIR 0,5000,0,6 @20:54:31.40 PuckHeater.configure :default @20:54:31.47 Gate.power :heat,:ON,limit to 5000mA @20:54:31.64 Mounted lysis Cartridge board#J59994 @20:54:31.68 Using default cartridge heat loss curve for HeatingFunctionInCarousel @20:54:33.38 SPR.command "read_sequences" @20:54:33.71 SPR.command "stats_on" @20:54:33.87 SPR.command "start 0 1" @20:54:34.57 SPR.start /var/log/esp/spr26jul2920h.spr.bz2 @20:54:36.56 Waiting for baseline SPR stabilization @20:54:36.58 Handoff.select :waste @20:54:37.10 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:54:43.80 Syringe tare is 51% of full scale at 23C (drifted -0%) @20:54:43.82 PinPort.in :locking @20:54:43.87 PinPort.seek :locked @20:54:44.71 Archive.in :fast @20:54:44.81 Lysis2.in :fast @20:54:44.86 Lysis1.seek 800uL @20:54:44.92 Archive.seek :empty @20:54:45.01 Lysis2.seek 800uL @20:54:53.49 delayUntil "21:04:36.14" @21:04:36.15 Lysis2.in :DAmix @21:04:36.26 Lysis1.in :DAmix @21:04:36.81 Lysis2.seek 550uL @21:04:36.90 Lysis1.seek 550uL @21:05:11.84 delay 5 seconds @21:05:16.86 Handoff.select SPR @21:05:17.36 SPR.command "start 2 14" @21:05:19.31 SPR stabilized @21:05:19.48 delay 20 seconds @21:05:39.50 SPR.command "start 2 0" @21:05:42.56 Waiting up to 11:00 for SPR to enter state 1 @21:07:40.23 SPR.command "hold_on" @21:07:41.61 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:07:42.28 Lysis2.seek 250uL @21:07:42.32 Lysis1.seek 250uL @21:08:24.28 delay 15 seconds @21:08:39.30 SPR.command "hold_off" @21:08:42.79 Waiting up to 1:00:00 for SPR to enter state 7 @21:24:58.79 SPRsummary:5.55RIU,236RIU,234RIU,344RIU after 1926s @21:39:15.84 delay 20 seconds @21:39:35.86 SPR.command "start 2 0" @21:39:38.94 Waiting up to 11:00 for SPR to enter state 1 @21:41:36.62 SPR.command "hold_on" @21:41:38.52 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:41:39.23 Lysis2.seek 100uL @21:41:39.26 Lysis1.seek 100uL @21:42:00.34 delay 15 seconds @21:42:15.37 SPR.command "hold_off" @21:42:18.79 Waiting up to 1:00:00 for SPR to enter state 7 @22:12:50.31 SPR analysis completed @22:12:50.86 Ended log spr26jul2920h.spr.bz2 @22:12:50.88 SPR.command "stats_off" @22:12:52.29 Handoff.select :close @22:12:52.80 Gate.power :heat,:OFF @22:12:52.82 Gate.power :cartridge,:OFF @22:12:52.90 PinPort.in :unlocking @22:12:52.95 PinPort.seek :clear @22:12:53.77 Carousel.in :default @22:12:53.90 Carousel.home.seek 48 @22:12:54.23 Plunge.seek :locked @22:12:54.41 Archive.in :unlocking @22:12:54.57 Archive.seek :clear @22:12:54.64 Air.in :unlocking @22:12:54.80 Air.seek :clear @22:12:54.91 Lysis1.in :unlocking @22:12:55.06 Lysis1.seek :clear @22:12:55.17 Lysis2.in :unlocking @22:12:55.29 Lysis2.seek :clear @22:12:59.91 Gate.power :motor,:OFF @22:12:59.93 Gate.power :analytic1,:OFF @22:12:59.94 Gate.power :core,:OFF @22:12:59.96 Gate.power :main,:OFF @22:12:59.97 Safely stopped and ready to power off @22:13:00.61 Gate.power :sampler,:OFF,limit to 3500mA @22:13:00.63 Gate.power :skimmer,:OFF,limit to 3500mA @22:13:03.77 Gate.power :main,:ON,limit to 5000mA @22:13:03.78 Gate.power :analytic1,:ON,limit to 4000mA @22:13:03.85 Powering core bus @22:13:06.22 SPR Module 1, FW Rev. B, FPGA Rev. C @22:13:06.64 Plunge.configure :default @22:13:06.72 SamplePump.configure :default @22:13:06.81 Toroid.configure :default @22:13:06.89 Carousel.configure :default @22:13:07.56 Abs.configure :default @22:13:07.64 PhaseSeparator.configure :default @22:13:07.72 Archive.configure :default @22:13:07.81 Air.configure :default @22:13:07.86 Lysis1.configure :default @22:13:07.92 Lysis2.configure :default @22:13:08.00 PinPort.configure :default @22:13:08.06 All elf controllers running firmware version 4.94 @22:13:08.08 Gate.power :motor,:ON,limit to 5000mA @22:13:08.39 Plunge.home.jog 0 @22:13:09.10 PinPort.home.jog 0 @22:13:09.18 Lysis2.home.jog 0 @22:13:09.23 Air.home.jog 0 @22:13:09.25 Carousel.home.jog 0 @22:13:09.29 Archive.home.jog 0 @22:13:09.32 Toroid.home.jog 0 @22:13:09.35 Lysis1.home.jog 0 @22:13:10.27 Plunge.in :homing @22:13:10.52 Toroid.in :homing @22:13:10.56 Plunge.jog 1486 @22:13:10.61 Lysis2.in :homing @22:13:10.65 Air.in :homing @22:13:10.67 Lysis1.in :homing @22:13:10.69 Archive.in :homing @22:13:10.72 PinPort.in :homing @22:13:10.98 Toroid.jog 1377 @22:13:11.02 Lysis2.jog 1621 @22:13:11.20 Air.jog 1432 @22:13:11.38 Lysis1.jog 1603 @22:13:11.44 Archive.jog 307 @22:13:11.48 PinPort.jog 1247 @22:13:12.06 Plunge.setRawPosition! 50 @22:13:12.11 Plunge.seek :home @22:13:12.23 Archive.setRawPosition! 50 @22:13:12.28 Archive.seek :home @22:13:12.40 Plunge.in :default @22:13:12.49 Lysis2.setRawPosition! 50 @22:13:12.57 Lysis2.seek :home @22:13:12.67 Air.setRawPosition! 0 @22:13:12.70 Archive.in :default @22:13:12.81 Air.seek :home @22:13:12.96 Lysis1.setRawPosition! 50 @22:13:12.99 Air.in :default @22:13:13.01 Lysis2.in :default @22:13:13.20 Lysis1.seek :home @22:13:13.37 PinPort.setRawPosition! 3150 @22:13:13.43 PinPort.seek :home @22:13:13.54 Lysis1.in :default @22:13:15.72 Toroid.setRawPosition! 0 @22:13:15.77 Toroid.seek :home @22:13:15.85 Toroid.in :default @22:13:17.85 PinPort.in :default @22:13:18.18 Toroid.seek :clear @22:13:18.84 PinPort.in :unlocking @22:13:18.93 PinPort.seek :clear @22:13:22.35 Air.seek :clear @22:13:22.45 Lysis1.seek :clear @22:13:23.20 Lysis2.seek :clear @22:13:23.98 Archive.seek :clear @22:13:24.77 Carousel.in :default @22:13:24.89 Carousel.home.seek 48 @22:13:25.19 Selecting Cartridge 42 @22:13:25.51 Carousel.seek 42 @22:13:27.09 Carousel.reconfigure :align @22:13:27.26 Lysis1.in :fast @22:13:27.31 Lysis1.seek :locked @22:13:29.95 Plunge.seek :locked @22:13:30.12 Air.in :fast @22:13:30.17 Air.seek :locked @22:13:31.24 Carousel.reconfigure :locked @22:13:31.41 Powering cartridge bus @22:13:34.08 Gate.setIR 1750,5000,0,6 @22:13:34.21 Gate.setIR 0,5000,0,6 @22:13:34.28 PuckHeater.configure :default @22:13:34.34 Gate.power :heat,:ON,limit to 5000mA @22:13:34.51 Mounted lysis Cartridge board#J59976 @22:13:34.56 Using default cartridge heat loss curve for HeatingFunctionInCarousel @22:13:36.10 SPR.command "read_sequences" @22:13:36.38 SPR.command "stats_on" @22:13:36.53 SPR.command "start 0 1" @22:13:37.24 SPR.start /var/log/esp/spr26jul2922h.spr.bz2 @22:13:39.23 Waiting for baseline SPR stabilization @22:13:39.25 Handoff.select :waste @22:13:39.75 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:13:46.41 Syringe tare is 51% of full scale at 23C (drifted -0%) @22:13:46.43 PinPort.in :locking @22:13:46.49 PinPort.seek :locked @22:13:47.36 Archive.in :fast @22:13:47.44 Lysis2.in :fast @22:13:47.49 Lysis1.seek 800uL @22:13:47.56 Archive.seek :empty @22:13:47.73 Lysis2.seek 800uL @22:13:56.06 delayUntil "22:23:38.80" @22:23:38.82 Lysis2.in :DAmix @22:23:38.93 Lysis1.in :DAmix @22:23:39.57 Lysis2.seek 550uL @22:23:39.61 Lysis1.seek 550uL @22:24:14.63 delay 5 seconds @22:24:19.65 Handoff.select SPR @22:24:20.14 SPR.command "start 2 14" @22:24:21.45 SPR stabilized @22:24:21.60 delay 20 seconds @22:24:41.62 SPR.command "start 2 0" @22:24:44.28 Waiting up to 11:00 for SPR to enter state 1 @22:26:41.96 SPR.command "hold_on" @22:26:43.86 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:26:44.50 Lysis2.seek 250uL @22:26:44.54 Lysis1.seek 250uL @22:27:26.50 delay 15 seconds @22:27:41.53 SPR.command "hold_off" @22:27:44.93 Waiting up to 1:00:00 for SPR to enter state 7 @22:43:59.56 SPRsummary:5.5RIU,283RIU,274RIU,395RIU after 1924s @22:58:16.62 delay 20 seconds @22:58:36.64 SPR.command "start 2 0" @22:58:39.72 Waiting up to 11:00 for SPR to enter state 1 @23:00:37.40 SPR.command "hold_on" @23:00:38.76 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:00:39.48 Lysis2.seek 100uL @23:00:39.56 Lysis1.seek 100uL @23:01:00.61 delay 15 seconds @23:01:15.64 SPR.command "hold_off" @23:01:19.17 Waiting up to 1:00:00 for SPR to enter state 7 @23:31:52.03 SPR analysis completed @23:31:52.58 Ended log spr26jul2922h.spr.bz2 @23:31:52.60 SPR.command "stats_off" @23:31:54.02 Handoff.select :close @23:31:54.54 Gate.power :heat,:OFF @23:31:54.56 Gate.power :cartridge,:OFF @23:31:54.64 PinPort.in :unlocking @23:31:54.70 PinPort.seek :clear @23:31:55.52 Carousel.in :default @23:31:55.64 Carousel.home.seek 42 @23:31:55.99 Plunge.seek :locked @23:31:56.17 Archive.in :unlocking @23:31:56.29 Archive.seek :clear @23:31:56.40 Air.in :unlocking @23:31:56.53 Air.seek :clear @23:31:56.59 Lysis1.in :unlocking @23:31:56.78 Lysis1.seek :clear @23:31:56.84 Lysis2.in :unlocking @23:31:56.95 Lysis2.seek :clear @23:32:01.58 Gate.power :motor,:OFF @23:32:01.59 Gate.power :analytic1,:OFF @23:32:01.61 Gate.power :core,:OFF @23:32:01.62 Gate.power :main,:OFF @23:32:01.63 Safely stopped and ready to power off @23:32:02.26 Gate.power :sampler,:OFF,limit to 3500mA @23:32:02.28 Gate.power :skimmer,:OFF,limit to 3500mA @23:32:05.43 Gate.power :main,:ON,limit to 5000mA @23:32:05.45 Gate.power :analytic1,:ON,limit to 4000mA @23:32:05.52 Powering core bus @23:32:07.88 SPR Module 1, FW Rev. B, FPGA Rev. C @23:32:08.86 Plunge.configure :default @23:32:08.95 SamplePump.configure :default @23:32:09.03 Toroid.configure :default @23:32:09.11 Carousel.configure :default @23:32:09.22 Abs.configure :default @23:32:09.31 PhaseSeparator.configure :default @23:32:09.39 Archive.configure :default @23:32:09.47 Air.configure :default @23:32:09.53 Lysis1.configure :default @23:32:09.58 Lysis2.configure :default @23:32:09.67 PinPort.configure :default @23:32:09.72 All elf controllers running firmware version 4.94 @23:32:09.75 Gate.power :motor,:ON,limit to 5000mA @23:32:09.97 Plunge.home.jog 0 @23:32:10.62 Air.home.jog 0 @23:32:10.75 Lysis2.home.jog 0 @23:32:10.78 Archive.home.jog 0 @23:32:10.83 Toroid.home.jog 0 @23:32:10.89 Lysis1.home.jog 0 @23:32:10.93 PinPort.home.jog 0 @23:32:10.96 Carousel.home.jog 0 @23:32:11.83 Plunge.in :homing @23:32:12.07 Plunge.jog 1494 @23:32:12.27 Air.in :homing @23:32:12.39 Lysis2.in :homing @23:32:12.41 PinPort.in :homing @23:32:12.43 Lysis1.in :homing @23:32:12.47 Toroid.in :homing @23:32:12.51 Archive.in :homing @23:32:12.65 Air.jog 1433 @23:32:12.81 Lysis2.jog 1621 @23:32:12.89 PinPort.jog 1242 @23:32:12.96 Lysis1.jog 1601 @23:32:13.02 Toroid.jog 1363 @23:32:13.09 Archive.jog 307 @23:32:13.45 Plunge.setRawPosition! 50 @23:32:13.51 Plunge.seek :home @23:32:13.79 Plunge.in :default @23:32:13.91 Archive.setRawPosition! 50 @23:32:13.96 Archive.seek :home @23:32:14.10 Air.setRawPosition! 0 @23:32:14.15 Air.seek :home @23:32:14.27 Archive.in :default @23:32:14.29 Air.in :default @23:32:14.44 Lysis2.setRawPosition! 50 @23:32:14.49 Lysis2.seek :home @23:32:14.58 Lysis1.setRawPosition! 50 @23:32:14.64 Lysis1.seek :home @23:32:14.78 Lysis2.in :default @23:32:14.92 Lysis1.in :default @23:32:15.00 PinPort.setRawPosition! 3150 @23:32:15.07 PinPort.seek :home @23:32:17.76 Toroid.setRawPosition! 0 @23:32:18.23 Toroid.seek :home @23:32:18.40 Toroid.in :default @23:32:19.45 PinPort.in :default @23:32:19.78 Toroid.seek :clear @23:32:19.97 PinPort.in :unlocking @23:32:20.02 PinPort.seek :clear @23:32:23.47 Air.seek :clear @23:32:23.57 Lysis1.seek :clear @23:32:24.29 Lysis2.seek :clear @23:32:25.08 Archive.seek :clear @23:32:25.86 Carousel.in :default @23:32:26.00 Carousel.home.seek 42 @23:32:26.27 Selecting Cartridge 36 @23:32:26.57 Carousel.seek 36 @23:32:28.15 Carousel.reconfigure :align @23:32:28.28 Lysis1.in :fast @23:32:28.34 Lysis1.seek :locked @23:32:28.41 Slide::Warning in core -- Storage Carousel (SC) reseek at 36 + 4 counts @23:32:31.02 Plunge.seek :locked @23:32:31.23 Air.in :fast @23:32:31.29 Air.seek :locked @23:32:32.36 Carousel.reconfigure :locked @23:32:32.52 Powering cartridge bus @23:32:35.23 Gate.setIR 1750,5000,0,6 @23:32:35.35 Gate.setIR 0,5000,0,6 @23:32:35.42 PuckHeater.configure :default @23:32:35.49 Gate.power :heat,:ON,limit to 5000mA @23:32:35.66 Mounted lysis Cartridge board#J59925 @23:32:35.70 Using default cartridge heat loss curve for HeatingFunctionInCarousel @23:32:37.26 SPR.command "read_sequences" @23:32:38.04 SPR.command "stats_on" @23:32:38.19 SPR.command "start 0 1" @23:32:38.90 SPR.start /var/log/esp/spr26jul2923h.spr.bz2 @23:32:40.88 Waiting for baseline SPR stabilization @23:32:40.91 Handoff.select :waste @23:32:41.42 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:32:48.08 Syringe tare is 51% of full scale at 22C (drifted -0%) @23:32:48.11 PinPort.in :locking @23:32:48.16 PinPort.seek :locked @23:32:49.01 Archive.in :fast @23:32:49.07 Lysis2.in :fast @23:32:49.21 Lysis1.seek 800uL @23:32:49.36 Lysis2.seek 800uL @23:32:49.42 Archive.seek :empty @23:32:57.80 delayUntil "23:42:40.46" @23:42:40.48 Lysis2.in :DAmix @23:42:40.59 Lysis1.in :DAmix @23:42:41.23 Lysis2.seek 550uL @23:42:41.32 Lysis1.seek 550uL @23:43:16.28 delay 5 seconds @23:43:21.30 Handoff.select SPR @23:43:21.79 SPR.command "start 2 14" @23:43:23.11 SPR stabilized @23:43:23.31 delay 20 seconds @23:43:43.34 SPR.command "start 2 0" @23:43:45.94 Waiting up to 11:00 for SPR to enter state 1 @23:45:43.62 SPR.command "hold_on" @23:45:45.52 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:45:46.14 Lysis2.seek 250uL @23:45:46.19 Lysis1.seek 250uL @23:46:28.13 delay 15 seconds @23:46:43.15 SPR.command "hold_off" @23:46:46.59 Waiting up to 1:00:00 for SPR to enter state 7 @00:03:01.24EDT30-Jul-26 SPRsummary:4.59RIU,214RIU,209RIU,310RIU after 1924s @00:17:18.27 delay 20 seconds @00:17:38.29 SPR.command "start 2 0" @00:17:41.36 Waiting up to 11:00 for SPR to enter state 1 @00:19:39.05 SPR.command "hold_on" @00:19:40.96 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:19:41.68 Lysis2.seek 100uL @00:19:41.76 Lysis1.seek 100uL @00:20:02.80 delay 15 seconds @00:20:17.82 SPR.command "hold_off" @00:20:21.22 Waiting up to 1:00:00 for SPR to enter state 7 @00:50:52.76 SPR analysis completed @00:50:53.30 Ended log spr26jul2923h.spr.bz2 @00:50:53.32 SPR.command "stats_off" @00:50:54.71 Handoff.select :close @00:50:55.24 Gate.power :heat,:OFF @00:50:55.26 Gate.power :cartridge,:OFF @00:50:55.34 PinPort.in :unlocking @00:50:55.40 PinPort.seek :clear @00:50:56.24 Carousel.in :default @00:50:56.37 Carousel.home.seek 36 @00:50:56.72 Plunge.seek :locked @00:50:56.89 Archive.in :unlocking @00:50:57.04 Archive.seek :clear @00:50:57.11 Air.in :unlocking @00:50:57.35 Air.seek :clear @00:50:57.40 Lysis1.in :unlocking @00:50:57.60 Lysis1.seek :clear @00:50:57.71 Lysis2.in :unlocking @00:50:57.78 Lysis2.seek :clear @00:51:02.39 Gate.power :motor,:OFF @00:51:02.41 Gate.power :analytic1,:OFF @00:51:02.43 Gate.power :core,:OFF @00:51:02.44 Gate.power :main,:OFF @00:51:02.45 Safely stopped and ready to power off @00:51:02.66 Gate.power :sampler,:OFF,limit to 3500mA @00:51:02.68 Gate.power :skimmer,:OFF,limit to 3500mA @00:51:06.25 Gate.power :main,:ON,limit to 5000mA @00:51:06.27 Gate.power :analytic1,:ON,limit to 4000mA @00:51:06.34 Powering core bus @00:51:08.70 SPR Module 1, FW Rev. B, FPGA Rev. C @00:51:09.76 Plunge.configure :default @00:51:09.84 SamplePump.configure :default @00:51:09.92 Toroid.configure :default @00:51:10.01 Carousel.configure :default @00:51:10.09 Abs.configure :default @00:51:10.17 PhaseSeparator.configure :default @00:51:10.27 Archive.configure :default @00:51:10.35 Air.configure :default @00:51:10.40 Lysis1.configure :default @00:51:10.46 Lysis2.configure :default @00:51:10.55 PinPort.configure :default @00:51:10.60 All elf controllers running firmware version 4.94 @00:51:10.62 Gate.power :motor,:ON,limit to 5000mA @00:51:11.11 Plunge.home.jog 0 @00:51:11.69 PinPort.home.jog 0 @00:51:11.74 Lysis1.home.jog 0 @00:51:11.77 Archive.home.jog 0 @00:51:11.82 Toroid.home.jog 0 @00:51:11.84 Lysis2.home.jog 0 @00:51:11.88 Carousel.home.jog 0 @00:51:11.91 Air.home.jog 0 @00:51:12.91 Plunge.in :homing @00:51:13.07 Plunge.jog 1493 @00:51:13.34 PinPort.in :homing @00:51:13.37 Lysis2.in :homing @00:51:13.39 Air.in :homing @00:51:13.41 Toroid.in :homing @00:51:13.44 Lysis1.in :homing @00:51:13.46 Archive.in :homing @00:51:13.72 Lysis2.jog 1622 @00:51:13.79 PinPort.jog 1250 @00:51:13.82 Air.jog 1432 @00:51:13.86 Toroid.jog 1364 @00:51:13.89 Archive.jog 306 @00:51:13.93 Lysis1.jog 1604 @00:51:14.40 Plunge.setRawPosition! 50 @00:51:14.46 Plunge.seek :home @00:51:14.75 Archive.setRawPosition! 50 @00:51:14.78 Plunge.in :default @00:51:14.89 Archive.seek :home @00:51:15.20 Archive.in :default @00:51:15.34 Lysis2.setRawPosition! 50 @00:51:15.35 Air.setRawPosition! 0 @00:51:15.48 Lysis2.seek :home @00:51:15.50 Air.seek :home @00:51:15.71 Air.in :default @00:51:15.72 Lysis1.setRawPosition! 50 @00:51:15.84 Lysis1.seek :home @00:51:15.98 PinPort.setRawPosition! 3150 @00:51:16.01 Lysis2.in :default @00:51:16.09 PinPort.seek :home @00:51:16.21 Lysis1.in :default @00:51:18.67 Toroid.setRawPosition! 0 @00:51:18.73 Toroid.seek :home @00:51:18.83 Toroid.in :default @00:51:20.49 PinPort.in :default @00:51:20.83 Toroid.seek :clear @00:51:20.99 PinPort.in :unlocking @00:51:21.05 PinPort.seek :clear @00:51:24.50 Air.seek :clear @00:51:24.59 Lysis1.seek :clear @00:51:25.32 Lysis2.seek :clear @00:51:26.11 Archive.seek :clear @00:51:26.89 Carousel.in :default @00:51:27.02 Carousel.home.seek 36 @00:51:27.28 Selecting Cartridge 30 @00:51:27.59 Carousel.seek 30 @00:51:29.20 Carousel.reconfigure :align @00:51:29.33 Lysis1.in :fast @00:51:29.39 Lysis1.seek :locked @00:51:29.47 Slide::Warning in core -- Storage Carousel (SC) reseek at 30 + 4 counts (sensed 30 + 5 counts) @00:51:32.09 Plunge.seek :locked @00:51:32.26 Air.in :fast @00:51:32.32 Air.seek :locked @00:51:33.39 Carousel.reconfigure :locked @00:51:33.55 Powering cartridge bus @00:51:36.22 Gate.setIR 1750,5000,0,6 @00:51:36.35 Gate.setIR 0,5000,0,6 @00:51:36.42 PuckHeater.configure :default @00:51:36.49 Gate.power :heat,:ON,limit to 5000mA @00:51:36.66 Mounted lysis Cartridge board#J59986 @00:51:36.69 Using default cartridge heat loss curve for HeatingFunctionInCarousel @00:51:38.28 SPR.command "read_sequences" @00:51:38.86 SPR.command "stats_on" @00:51:39.01 SPR.command "start 0 1" @00:51:39.72 SPR.start /var/log/esp/spr26jul3000h.spr.bz2 @00:51:42.23 Waiting for baseline SPR stabilization @00:51:42.26 Handoff.select :waste @00:51:42.76 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:51:59.40 Syringe tare is 51% of full scale at 22C (drifted -0%) @00:51:59.42 PinPort.in :locking @00:51:59.48 PinPort.seek :locked @00:52:00.32 Archive.in :fast @00:52:00.39 Lysis2.in :fast @00:52:00.46 Lysis1.seek 800uL @00:52:00.61 Lysis2.seek 800uL @00:52:00.67 Archive.seek :empty @00:52:09.09 delayUntil "01:01:42.20" @01:01:42.27 Lysis2.in :DAmix @01:01:42.48 Lysis1.in :DAmix @01:01:43.00 Lysis2.seek 550uL @01:01:43.04 Lysis1.seek 550uL @01:02:18.06 delay 5 seconds @01:02:23.08 Handoff.select SPR @01:02:23.59 SPR.command "start 2 14" @01:02:24.88 SPR stabilized @01:02:25.04 delay 20 seconds @01:02:45.06 SPR.command "start 2 0" @01:02:47.71 Waiting up to 11:00 for SPR to enter state 1 @01:04:45.39 SPR.command "hold_on" @01:04:46.76 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:04:47.46 Lysis2.seek 250uL @01:04:47.50 Lysis1.seek 250uL @01:05:29.45 delay 15 seconds @01:05:44.47 SPR.command "hold_off" @01:05:47.40 Waiting up to 1:00:00 for SPR to enter state 7 @01:22:02.99 SPRsummary:5.31RIU,155RIU,144RIU,219RIU after 1925s @01:36:20.03 delay 20 seconds @01:36:40.05 SPR.command "start 2 0" @01:36:43.19 Waiting up to 11:00 for SPR to enter state 1 @01:38:40.82 SPR.command "hold_on" @01:38:42.19 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:38:42.92 Lysis2.seek 100uL @01:38:42.97 Lysis1.seek 100uL @01:39:04.01 delay 15 seconds @01:39:19.03 SPR.command "hold_off" @01:39:22.04 Waiting up to 1:00:00 for SPR to enter state 7 @02:09:54.53 SPR analysis completed @02:09:55.07 Ended log spr26jul3000h.spr.bz2 @02:09:55.09 SPR.command "stats_off" @02:09:56.48 Handoff.select :close @02:09:57.00 Gate.power :heat,:OFF @02:09:57.02 Gate.power :cartridge,:OFF @02:09:57.10 PinPort.in :unlocking @02:09:57.18 PinPort.seek :clear @02:09:58.00 Carousel.in :default @02:09:58.12 Carousel.home.seek 30 @02:09:58.46 Plunge.seek :locked @02:09:58.65 Archive.in :unlocking @02:09:58.80 Archive.seek :clear @02:09:58.85 Air.in :unlocking @02:09:59.04 Air.seek :clear @02:09:59.23 Lysis1.in :unlocking @02:09:59.35 Lysis1.seek :clear @02:09:59.46 Lysis2.in :unlocking @02:09:59.52 Lysis2.seek :clear @02:10:04.13 Gate.power :motor,:OFF @02:10:04.15 Gate.power :analytic1,:OFF @02:10:04.16 Gate.power :core,:OFF @02:10:04.17 Gate.power :main,:OFF @02:10:04.19 Safely stopped and ready to power off @02:10:04.39 Gate.power :sampler,:OFF,limit to 3500mA @02:10:04.41 Gate.power :skimmer,:OFF,limit to 3500mA @02:10:07.98 Gate.power :main,:ON,limit to 5000mA @02:10:08.00 Gate.power :analytic1,:ON,limit to 4000mA @02:10:08.07 Powering core bus @02:10:10.43 SPR Module 1, FW Rev. B, FPGA Rev. C @02:10:11.47 Plunge.configure :default @02:10:11.56 SamplePump.configure :default @02:10:11.64 Toroid.configure :default @02:10:11.73 Carousel.configure :default @02:10:11.81 Abs.configure :default @02:10:11.90 PhaseSeparator.configure :default @02:10:11.98 Archive.configure :default @02:10:12.06 Air.configure :default @02:10:12.11 Lysis1.configure :default @02:10:12.17 Lysis2.configure :default @02:10:12.25 PinPort.configure :default @02:10:12.30 All elf controllers running firmware version 4.94 @02:10:12.33 Gate.power :motor,:ON,limit to 5000mA @02:10:12.75 Plunge.home.jog 0 @02:10:13.37 PinPort.home.jog 0 @02:10:13.41 Lysis1.home.jog 0 @02:10:13.46 Archive.home.jog 0 @02:10:13.50 Toroid.home.jog 0 @02:10:13.54 Lysis2.home.jog 0 @02:10:13.57 Air.home.jog 0 @02:10:13.60 Carousel.home.jog 0 @02:10:14.41 Plunge.in :homing @02:10:14.74 Plunge.jog 1492 @02:10:14.96 PinPort.in :homing @02:10:15.00 Lysis2.in :homing @02:10:15.02 Archive.in :homing @02:10:15.04 Air.in :homing @02:10:15.06 Toroid.in :homing @02:10:15.08 Lysis1.in :homing @02:10:15.38 Lysis2.jog 1623 @02:10:15.42 PinPort.jog 1258 @02:10:15.48 Archive.jog 308 @02:10:15.52 Air.jog 1432 @02:10:15.67 Toroid.jog 1357 @02:10:15.74 Lysis1.jog 275 @02:10:16.15 Plunge.setRawPosition! 50 @02:10:16.20 Plunge.seek :home @02:10:16.37 Archive.setRawPosition! 50 @02:10:16.43 Archive.seek :home @02:10:16.56 Lysis1.setRawPosition! 50 @02:10:16.58 Plunge.in :default @02:10:16.69 Lysis1.seek :home @02:10:16.81 Archive.in :default @02:10:16.90 Lysis2.setRawPosition! 50 @02:10:17.01 Lysis1.in :default @02:10:17.05 Lysis2.seek :home @02:10:17.23 Air.setRawPosition! 0 @02:10:17.28 Air.seek :home @02:10:17.41 Air.in :default @02:10:17.43 Lysis2.in :default @02:10:17.57 PinPort.setRawPosition! 3150 @02:10:17.63 PinPort.seek :home @02:10:20.38 Toroid.setRawPosition! 0 @02:10:20.87 Toroid.seek :home @02:10:21.05 Toroid.in :default @02:10:22.00 PinPort.in :default @02:10:22.33 Toroid.seek :clear @02:10:22.51 PinPort.in :unlocking @02:10:22.56 PinPort.seek :clear @02:10:26.02 Air.seek :clear @02:10:26.12 Lysis1.seek :clear @02:10:26.84 Lysis2.seek :clear @02:10:27.63 Archive.seek :clear @02:10:28.41 Carousel.in :default @02:10:28.54 Carousel.home.seek 30 @02:10:28.81 Selecting Cartridge 24 @02:10:29.11 Carousel.seek 24 @02:10:30.71 Carousel.reconfigure :align @02:10:30.84 Lysis1.in :fast @02:10:30.90 Lysis1.seek :locked @02:10:33.51 Plunge.seek :locked @02:10:33.67 Air.in :fast @02:10:33.73 Air.seek :locked @02:10:34.80 Carousel.reconfigure :locked @02:10:35.02 Powering cartridge bus @02:10:37.70 Gate.setIR 1750,5000,0,6 @02:10:37.83 Gate.setIR 0,5000,0,6 @02:10:37.90 PuckHeater.configure :default @02:10:37.97 Gate.power :heat,:ON,limit to 5000mA @02:10:38.14 Mounted lysis Cartridge board#J59995 @02:10:38.18 Using default cartridge heat loss curve for HeatingFunctionInCarousel @02:10:39.74 SPR.command "read_sequences" @02:10:39.80 SPR.command "stats_on" @02:10:40.70 SPR.command "start 0 1" @02:10:41.40 SPR.start /var/log/esp/spr26jul3002h.spr.bz2 @02:10:43.45 Waiting for baseline SPR stabilization @02:10:43.46 Handoff.select :waste @02:10:43.97 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:11:03.59 Syringe tare is 51% of full scale at 22C (drifted -0%) @02:11:03.62 PinPort.in :locking @02:11:03.67 PinPort.seek :locked @02:11:04.51 Archive.in :fast @02:11:04.60 Lysis2.in :fast @02:11:04.65 Lysis1.seek 800uL @02:11:04.68 Archive.seek :empty @02:11:04.88 Lysis2.seek 800uL @02:11:13.15 delayUntil "02:20:43.02" @02:20:43.03 Lysis2.in :DAmix @02:20:43.18 Lysis1.in :DAmix @02:20:43.74 Lysis2.seek 550uL @02:20:43.78 Lysis1.seek 550uL @02:21:18.75 delay 5 seconds @02:21:23.77 Handoff.select SPR @02:21:24.27 SPR.command "start 2 14" @02:21:25.67 SPR stabilized @02:21:25.83 delay 20 seconds @02:21:45.86 SPR.command "start 2 0" @02:21:48.50 Waiting up to 11:00 for SPR to enter state 1 @02:23:46.17 SPR.command "hold_on" @02:23:47.55 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @02:23:48.21 Lysis2.seek 250uL @02:23:48.26 Lysis1.seek 250uL @02:24:30.18 delay 15 seconds @02:24:45.20 SPR.command "hold_off" @02:24:48.73 Waiting up to 1:00:00 for SPR to enter state 7 @02:41:04.72 SPRsummary:5.4RIU,55.7RIU,42RIU,83.7RIU after 1925s @02:55:21.76 delay 20 seconds @02:55:41.79 SPR.command "start 2 0" @02:55:45.40 Waiting up to 11:00 for SPR to enter state 1 @02:57:43.50 SPR.command "hold_on" @02:57:45.40 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:57:46.12 Lysis2.seek 100uL @02:57:46.16 Lysis1.seek 100uL @02:58:07.25 delay 15 seconds @02:58:22.28 SPR.command "hold_off" @02:58:25.67 Waiting up to 1:00:00 for SPR to enter state 7 @03:28:57.26 SPR analysis completed @03:28:57.78 Ended log spr26jul3002h.spr.bz2 @03:28:57.80 SPR.command "stats_off" @03:28:59.13 Handoff.select :close @03:28:59.69 Gate.power :heat,:OFF @03:28:59.71 Gate.power :cartridge,:OFF @03:28:59.79 PinPort.in :unlocking @03:28:59.84 PinPort.seek :clear @03:29:00.66 Carousel.in :default @03:29:00.79 Carousel.home.seek 24 @03:29:01.15 Plunge.seek :locked @03:29:01.36 Archive.in :unlocking @03:29:01.52 Archive.seek :clear @03:29:01.61 Air.in :unlocking @03:29:01.78 Air.seek :clear @03:29:01.85 Lysis1.in :unlocking @03:29:02.01 Lysis1.seek :clear @03:29:02.14 Lysis2.in :unlocking @03:29:02.20 Lysis2.seek :clear @03:29:06.82 Gate.power :motor,:OFF @03:29:06.84 Gate.power :analytic1,:OFF @03:29:06.85 Gate.power :core,:OFF @03:29:06.87 Gate.power :main,:OFF @03:29:06.88 Safely stopped and ready to power off @03:29:07.08 Gate.power :sampler,:OFF,limit to 3500mA @03:29:07.09 Gate.power :skimmer,:OFF,limit to 3500mA @03:29:10.68 Gate.power :main,:ON,limit to 5000mA @03:29:10.69 Gate.power :analytic1,:ON,limit to 4000mA @03:29:10.76 Powering core bus @03:29:13.12 SPR Module 1, FW Rev. B, FPGA Rev. C @03:29:13.54 Plunge.configure :default @03:29:14.20 SamplePump.configure :default @03:29:14.29 Toroid.configure :default @03:29:14.37 Carousel.configure :default @03:29:14.45 Abs.configure :default @03:29:14.53 PhaseSeparator.configure :default @03:29:14.61 Archive.configure :default @03:29:14.69 Air.configure :default @03:29:14.75 Lysis1.configure :default @03:29:14.80 Lysis2.configure :default @03:29:14.88 PinPort.configure :default @03:29:14.94 All elf controllers running firmware version 4.94 @03:29:14.96 Gate.power :motor,:ON,limit to 5000mA @03:29:15.69 Lysis1.home.jog 0 @03:29:15.87 Lysis2.home.jog 0 @03:29:15.90 Plunge.home.jog 0 @03:29:15.93 Carousel.home.jog 0 @03:29:15.99 Air.home.jog 0 @03:29:16.01 Toroid.home.jog 0 @03:29:16.07 Archive.home.jog 0 @03:29:16.86 PinPort.home.jog 0 @03:29:17.46 Lysis2.in :homing @03:29:17.51 Air.in :homing @03:29:17.56 Toroid.in :homing @03:29:17.58 Archive.in :homing @03:29:17.60 Plunge.in :homing @03:29:17.62 Lysis1.in :homing @03:29:17.85 Lysis2.jog 1615 @03:29:17.88 Air.jog 1433 @03:29:17.94 Toroid.jog 1348 @03:29:18.10 Archive.jog 309 @03:29:18.16 Lysis1.jog 1604 @03:29:18.20 Plunge.jog 1486 @03:29:18.58 PinPort.in :homing @03:29:18.64 PinPort.jog 1254 @03:29:18.92 Archive.setRawPosition! 50 @03:29:18.98 Archive.seek :home @03:29:19.26 Archive.in :default @03:29:19.36 Air.setRawPosition! 0 @03:29:19.40 Lysis2.setRawPosition! 50 @03:29:19.50 Lysis2.seek :home @03:29:19.53 Air.seek :home @03:29:19.75 Plunge.setRawPosition! 50 @03:29:19.78 Lysis1.setRawPosition! 50 @03:29:19.80 Air.in :default @03:29:19.95 Plunge.seek :home @03:29:19.98 Lysis1.seek :home @03:29:20.17 Lysis2.in :default @03:29:20.29 Plunge.in :default @03:29:20.38 Lysis1.in :default @03:29:20.47 PinPort.setRawPosition! 3150 @03:29:20.53 PinPort.seek :home @03:29:22.76 Toroid.setRawPosition! 0 @03:29:22.82 Toroid.seek :home @03:29:23.38 Toroid.in :default @03:29:24.90 PinPort.in :default @03:29:25.24 Toroid.seek :clear @03:29:25.41 PinPort.in :unlocking @03:29:25.48 PinPort.seek :clear @03:29:28.92 Air.seek :clear @03:29:29.02 Lysis1.seek :clear @03:29:29.75 Lysis2.seek :clear @03:29:30.53 Archive.seek :clear @03:29:31.31 Carousel.in :default @03:29:31.44 Carousel.home.seek 24 @03:29:31.75 Selecting Cartridge 17 @03:29:32.36 Carousel.seek 17 @03:29:34.19 Carousel.reconfigure :align @03:29:34.31 Lysis1.in :fast @03:29:34.37 Lysis1.seek :locked @03:29:34.46 Slide::Warning in core -- Storage Carousel (SC) reseek at 17 + 5 counts (sensed 17 + 6 counts) @03:29:37.00 Plunge.seek :locked @03:29:37.22 Air.in :fast @03:29:37.28 Air.seek :locked @03:29:38.35 Carousel.reconfigure :locked @03:29:38.51 Powering cartridge bus @03:29:41.21 Gate.setIR 1750,5000,0,6 @03:29:41.34 Gate.setIR 0,5000,0,6 @03:29:41.41 PuckHeater.configure :default @03:29:41.47 Gate.power :heat,:ON,limit to 5000mA @03:29:41.65 Mounted lysis Cartridge board#J59993 @03:29:41.68 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:29:43.23 SPR.command "read_sequences" @03:29:43.29 SPR.command "stats_on" @03:29:43.44 SPR.command "start 0 1" @03:29:44.15 SPR.start /var/log/esp/spr26jul3003h.spr.bz2 @03:29:46.14 Waiting for baseline SPR stabilization @03:29:46.17 Handoff.select :waste @03:29:46.67 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:29:53.35 Syringe tare is 51% of full scale at 22C (drifted -0%) @03:29:53.38 PinPort.in :locking @03:29:53.43 PinPort.seek :locked @03:29:54.28 Archive.in :fast @03:29:54.38 Lysis2.in :fast @03:29:54.42 Lysis1.seek 800uL @03:29:54.47 Archive.seek :empty @03:29:54.57 Lysis2.seek 800uL @03:30:03.01 delayUntil "03:39:45.71" @03:39:45.73 Lysis2.in :DAmix @03:39:45.84 Lysis1.in :DAmix @03:39:46.45 Lysis2.seek 550uL @03:39:46.51 Lysis1.seek 550uL @03:40:21.47 delay 5 seconds @03:40:26.49 Handoff.select SPR @03:40:27.00 SPR.command "start 2 14" @03:40:28.35 SPR stabilized @03:40:28.52 delay 20 seconds @03:40:48.54 SPR.command "start 2 0" @03:40:51.22 Waiting up to 11:00 for SPR to enter state 1 @03:42:48.86 SPR.command "hold_on" @03:42:50.77 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @03:42:51.44 Lysis2.seek 250uL @03:42:51.50 Lysis1.seek 250uL @03:43:33.42 delay 15 seconds @03:43:48.45 SPR.command "hold_off" @03:43:52.37 Waiting up to 1:00:00 for SPR to enter state 7 @04:00:07.42 SPRsummary:5.94RIU,24.8RIU,16.1RIU,38.5RIU after 1925s @04:14:24.47 delay 20 seconds @04:14:44.49 SPR.command "start 2 0" @04:14:47.57 Waiting up to 11:00 for SPR to enter state 1 @04:16:45.25 SPR.command "hold_on" @04:16:46.62 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @04:16:47.37 Lysis2.seek 100uL @04:16:47.43 Lysis1.seek 100uL @04:17:08.48 delay 15 seconds @04:17:23.51 SPR.command "hold_off" @04:17:26.47 Waiting up to 1:00:00 for SPR to enter state 7 @04:47:58.95 SPR analysis completed @04:47:59.55 Ended log spr26jul3003h.spr.bz2 @04:47:59.57 SPR.command "stats_off" @04:48:00.85 Handoff.select :close @04:48:01.37 Gate.power :heat,:OFF @04:48:01.39 Gate.power :cartridge,:OFF @04:48:01.47 PinPort.in :unlocking @04:48:01.53 PinPort.seek :clear @04:48:02.35 Carousel.in :default @04:48:02.48 Carousel.home.seek 17 @04:48:02.81 Plunge.seek :locked @04:48:02.99 Archive.in :unlocking @04:48:03.20 Archive.seek :clear @04:48:03.26 Air.in :unlocking @04:48:03.46 Air.seek :clear @04:48:03.52 Lysis1.in :unlocking @04:48:03.71 Lysis1.seek :clear @04:48:03.84 Lysis2.in :unlocking @04:48:03.90 Lysis2.seek :clear @04:48:08.99 Gate.power :motor,:OFF @04:48:09.03 Gate.power :analytic1,:OFF @04:48:09.05 Gate.power :core,:OFF @04:48:09.06 Gate.power :main,:OFF @04:48:09.07 Safely stopped and ready to power off @04:48:09.31 Gate.power :sampler,:OFF,limit to 3500mA @04:48:09.33 Gate.power :skimmer,:OFF,limit to 3500mA @04:48:12.87 Gate.power :main,:ON,limit to 5000mA @04:48:12.89 Gate.power :analytic1,:ON,limit to 4000mA @04:48:12.97 Powering core bus @04:48:15.33 SPR Module 1, FW Rev. B, FPGA Rev. C @04:48:15.77 Plunge.configure :default @04:48:15.85 SamplePump.configure :default @04:48:15.94 Toroid.configure :default @04:48:16.02 Carousel.configure :default @04:48:16.10 Abs.configure :default @04:48:16.18 PhaseSeparator.configure :default @04:48:16.26 Archive.configure :default @04:48:16.35 Air.configure :default @04:48:16.41 Lysis1.configure :default @04:48:16.46 Lysis2.configure :default @04:48:16.54 PinPort.configure :default @04:48:16.60 All elf controllers running firmware version 4.94 @04:48:16.63 Gate.power :motor,:ON,limit to 5000mA @04:48:17.01 Plunge.home.jog 0 @04:48:17.71 PinPort.home.jog 0 @04:48:17.75 Lysis1.home.jog 0 @04:48:17.77 Carousel.home.jog 0 @04:48:17.80 Air.home.jog 0 @04:48:17.83 Archive.home.jog 0 @04:48:17.86 Lysis2.home.jog 0 @04:48:17.91 Toroid.home.jog 0 @04:48:18.80 Plunge.in :homing @04:48:19.06 Plunge.jog 1493 @04:48:19.13 Lysis2.in :homing @04:48:19.21 Air.in :homing @04:48:19.24 Toroid.in :homing @04:48:19.26 PinPort.in :homing @04:48:19.28 Lysis1.in :homing @04:48:19.30 Archive.in :homing @04:48:19.63 Lysis2.jog 1621 @04:48:19.69 Air.jog 1432 @04:48:19.72 PinPort.jog 1247 @04:48:19.75 Toroid.jog 1352 @04:48:19.78 Lysis1.jog 275 @04:48:19.84 Archive.jog 312 @04:48:20.54 Lysis1.setRawPosition! 50 @04:48:20.60 Lysis1.seek :home @04:48:20.70 Plunge.setRawPosition! 50 @04:48:20.77 Plunge.seek :home @04:48:20.86 Archive.setRawPosition! 50 @04:48:20.93 Archive.seek :home @04:48:21.04 Lysis1.in :default @04:48:21.22 Air.setRawPosition! 0 @04:48:21.25 Plunge.in :default @04:48:21.27 Lysis2.setRawPosition! 50 @04:48:21.43 Lysis2.seek :home @04:48:21.45 Air.seek :home @04:48:22.25 PinPort.setRawPosition! 3150 @04:48:22.28 Air.in :default @04:48:22.30 Archive.in :default @04:48:22.47 PinPort.seek :home @04:48:22.59 Lysis2.in :default @04:48:24.58 Toroid.setRawPosition! 0 @04:48:24.63 Toroid.seek :home @04:48:24.72 Toroid.in :default @04:48:26.85 PinPort.in :default @04:48:27.23 Toroid.seek :clear @04:48:27.40 PinPort.in :unlocking @04:48:27.45 PinPort.seek :clear @04:48:30.90 Air.seek :clear @04:48:30.99 Lysis1.seek :clear @04:48:31.71 Lysis2.seek :clear @04:48:32.50 Archive.seek :clear @04:48:33.28 Carousel.in :default @04:48:33.41 Carousel.home.seek 17 @04:48:33.68 Selecting Cartridge 12 @04:48:33.98 Carousel.seek 12 @04:48:35.35 Carousel.reconfigure :align @04:48:35.48 Lysis1.in :fast @04:48:35.53 Lysis1.seek :locked @04:48:38.16 Plunge.seek :locked @04:48:38.33 Air.in :fast @04:48:38.39 Air.seek :locked @04:48:39.46 Carousel.reconfigure :locked @04:48:39.61 Powering cartridge bus @04:48:42.28 Gate.setIR 1750,5000,0,6 @04:48:42.41 Gate.setIR 0,5000,0,6 @04:48:42.48 PuckHeater.configure :default @04:48:42.54 Gate.power :heat,:ON,limit to 5000mA @04:48:42.71 Mounted lysis Cartridge board#J59971 @04:48:42.75 Using default cartridge heat loss curve for HeatingFunctionInCarousel @04:48:44.32 SPR.command "read_sequences" @04:48:44.54 SPR.command "stats_on" @04:48:44.69 SPR.command "start 0 1" @04:48:45.45 SPR.start /var/log/esp/spr26jul3004h.spr.bz2 @04:48:47.40 Waiting for baseline SPR stabilization @04:48:47.42 Handoff.select :waste @04:48:47.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:48:54.57 Syringe tare is 51% of full scale at 22C (drifted -0%) @04:48:54.60 PinPort.in :locking @04:48:54.65 PinPort.seek :locked @04:48:55.49 Archive.in :fast @04:48:55.58 Lysis2.in :fast @04:48:55.67 Lysis1.seek 800uL @04:48:55.80 Archive.seek :empty @04:48:55.86 Lysis2.seek 800uL @04:49:04.28 delayUntil "04:58:46.96" @04:58:46.98 Lysis2.in :DAmix @04:58:47.10 Lysis1.in :DAmix @04:58:47.76 Lysis2.seek 550uL @04:58:47.82 Lysis1.seek 550uL @04:59:22.80 delay 5 seconds @04:59:27.82 Handoff.select SPR @04:59:28.32 SPR.command "start 2 14" @04:59:29.62 SPR stabilized @04:59:29.77 delay 20 seconds @04:59:49.79 SPR.command "start 2 0" @04:59:52.43 Waiting up to 11:00 for SPR to enter state 1 @05:01:50.12 SPR.command "hold_on" @05:01:52.03 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:01:52.68 Lysis2.seek 250uL @05:01:52.72 Lysis1.seek 250uL @05:02:34.64 delay 15 seconds @05:02:49.68 SPR.command "hold_off" @05:02:53.10 Waiting up to 1:00:00 for SPR to enter state 7 @05:19:07.73 SPRsummary:5.74RIU,13.5RIU,3.4RIU,19.2RIU after 1924s @05:33:24.77 delay 20 seconds @05:33:44.79 SPR.command "start 2 0" @05:33:48.41 Waiting up to 11:00 for SPR to enter state 1 @05:35:46.50 SPR.command "hold_on" @05:35:47.88 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:35:48.61 Lysis2.seek 100uL @05:35:48.67 Lysis1.seek 100uL @05:36:09.75 delay 15 seconds @05:36:24.77 SPR.command "hold_off" @05:36:27.71 Waiting up to 1:00:00 for SPR to enter state 7 @06:07:00.21 SPR analysis completed @06:07:00.74 Ended log spr26jul3004h.spr.bz2 @06:07:00.76 SPR.command "stats_off" @06:07:02.19 Handoff.select :close @06:07:02.71 Gate.power :heat,:OFF @06:07:02.72 Gate.power :cartridge,:OFF @06:07:02.81 PinPort.in :unlocking @06:07:02.87 PinPort.seek :clear @06:07:03.69 Carousel.in :default @06:07:03.82 Carousel.home.seek 12 @06:07:04.15 Plunge.seek :locked @06:07:04.33 Archive.in :unlocking @06:07:04.45 Archive.seek :clear @06:07:04.57 Air.in :unlocking @06:07:04.70 Air.seek :clear @06:07:04.77 Lysis1.in :unlocking @06:07:04.95 Lysis1.seek :clear @06:07:05.06 Lysis2.in :unlocking @06:07:05.12 Lysis2.seek :clear @06:07:09.75 Gate.power :motor,:OFF @06:07:10.24 Gate.power :analytic1,:OFF @06:07:10.29 Gate.power :core,:OFF @06:07:10.30 Gate.power :main,:OFF @06:07:10.31 Safely stopped and ready to power off @06:07:10.54 Gate.power :sampler,:OFF,limit to 3500mA @06:07:10.56 Gate.power :skimmer,:OFF,limit to 3500mA @06:07:14.11 Gate.power :main,:ON,limit to 5000mA @06:07:14.13 Gate.power :analytic1,:ON,limit to 4000mA @06:07:14.21 Powering core bus @06:07:16.57 SPR Module 1, FW Rev. B, FPGA Rev. C @06:07:17.02 Plunge.configure :default @06:07:17.10 SamplePump.configure :default @06:07:17.21 Toroid.configure :default @06:07:17.30 Carousel.configure :default @06:07:17.38 Abs.configure :default @06:07:17.46 PhaseSeparator.configure :default @06:07:17.54 Archive.configure :default @06:07:17.63 Air.configure :default @06:07:17.69 Lysis1.configure :default @06:07:17.74 Lysis2.configure :default @06:07:17.83 PinPort.configure :default @06:07:17.88 All elf controllers running firmware version 4.94 @06:07:17.91 Gate.power :motor,:ON,limit to 5000mA @06:07:18.13 Plunge.home.jog 0 @06:07:18.87 Lysis2.home.jog 0 @06:07:18.93 Air.home.jog 0 @06:07:18.97 Carousel.home.jog 0 @06:07:19.01 Archive.home.jog 0 @06:07:19.04 Toroid.home.jog 0 @06:07:19.06 Lysis1.home.jog 0 @06:07:19.09 PinPort.home.jog 0 @06:07:20.01 Plunge.in :homing @06:07:20.27 Plunge.jog 1495 @06:07:20.49 PinPort.in :homing @06:07:20.52 Lysis2.in :homing @06:07:20.56 Air.in :homing @06:07:20.59 Toroid.in :homing @06:07:20.62 Lysis1.in :homing @06:07:20.64 Archive.in :homing @06:07:20.93 PinPort.jog 1245 @06:07:20.98 Lysis2.jog 1622 @06:07:21.03 Air.jog 1431 @06:07:21.06 Lysis1.jog 275 @06:07:21.08 Toroid.jog 1355 @06:07:21.14 Archive.jog 314 @06:07:21.61 Plunge.setRawPosition! 50 @06:07:21.67 Plunge.seek :home @06:07:21.88 Lysis1.setRawPosition! 50 @06:07:21.98 Archive.setRawPosition! 50 @06:07:22.02 Plunge.in :default @06:07:22.04 Lysis1.seek :home @06:07:22.21 Archive.seek :home @06:07:22.45 Lysis1.in :default @06:07:22.58 Archive.in :default @06:07:22.61 Air.setRawPosition! 0 @06:07:22.70 Air.seek :home @06:07:23.34 Lysis2.setRawPosition! 50 @06:07:23.46 PinPort.setRawPosition! 3150 @06:07:23.55 Lysis2.seek :home @06:07:23.63 PinPort.seek :home @06:07:23.81 Air.in :default @06:07:23.91 Lysis2.in :default @06:07:25.84 Toroid.setRawPosition! 0 @06:07:25.90 Toroid.seek :home @06:07:25.98 Toroid.in :default @06:07:28.06 PinPort.in :default @06:07:28.40 Toroid.seek :clear @06:07:28.58 PinPort.in :unlocking @06:07:28.64 PinPort.seek :clear @06:07:32.03 Air.seek :clear @06:07:32.12 Lysis1.seek :clear @06:07:32.85 Lysis2.seek :clear @06:07:33.64 Archive.seek :clear @06:07:34.43 Carousel.in :default @06:07:34.55 Carousel.home.seek 12 @06:07:34.82 Selecting Cartridge 6 @06:07:35.13 Carousel.seek 6 @06:07:36.74 Carousel.reconfigure :align @06:07:36.86 Lysis1.in :fast @06:07:36.91 Lysis1.seek :locked @06:07:39.54 Plunge.seek :locked @06:07:39.72 Air.in :fast @06:07:39.77 Air.seek :locked @06:07:40.84 Carousel.reconfigure :locked @06:07:41.01 Powering cartridge bus @06:07:43.68 Gate.setIR 1750,5000,0,6 @06:07:43.81 Gate.setIR 0,5000,0,6 @06:07:43.88 PuckHeater.configure :default @06:07:43.95 Gate.power :heat,:ON,limit to 5000mA @06:07:44.12 Mounted lysis Cartridge board#J59943 @06:07:44.15 Using default cartridge heat loss curve for HeatingFunctionInCarousel @06:07:45.70 SPR.command "read_sequences" @06:07:45.78 SPR.command "stats_on" @06:07:46.44 SPR.command "start 0 1" @06:07:46.64 SPR.start /var/log/esp/spr26jul3006h.spr.bz2 @06:07:48.63 Waiting for baseline SPR stabilization @06:07:48.65 Handoff.select :waste @06:07:49.20 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @06:07:59.34 Syringe tare is 51% of full scale at 22C (drifted 0%) @06:07:59.36 PinPort.in :locking @06:07:59.41 PinPort.seek :locked @06:08:00.25 Archive.in :fast @06:08:00.35 Lysis2.in :fast @06:08:00.40 Lysis1.seek 800uL @06:08:00.43 Archive.seek :empty @06:08:00.64 Lysis2.seek 800uL @06:08:08.90 delayUntil "06:17:48.20" @06:17:48.22 Lysis2.in :DAmix @06:17:48.33 Lysis1.in :DAmix @06:17:48.98 Lysis2.seek 550uL @06:17:49.05 Lysis1.seek 550uL @06:18:24.01 delay 5 seconds @06:18:29.03 Handoff.select SPR @06:18:29.53 SPR.command "start 2 14" @06:18:30.86 SPR stabilized @06:18:31.01 delay 20 seconds @06:18:51.03 SPR.command "start 2 0" @06:18:53.67 Waiting up to 11:00 for SPR to enter state 1 @06:20:51.36 SPR.command "hold_on" @06:20:52.74 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @06:20:53.45 Lysis2.seek 250uL @06:20:53.54 Lysis1.seek 250uL @06:21:35.40 delay 15 seconds @06:21:50.42 SPR.command "hold_off" @06:21:53.91 Waiting up to 1:00:00 for SPR to enter state 7 @06:38:09.92 SPRsummary:6.69RIU,12.1RIU,-0.432RIU,16.6RIU after 1925s @06:52:26.97 delay 20 seconds @06:52:46.99 SPR.command "start 2 0" @06:52:50.07 Waiting up to 11:00 for SPR to enter state 1 @06:54:47.73 SPR.command "hold_on" @06:54:49.12 SPR Dilution 2 of 2 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @06:54:49.90 Lysis2.seek 100uL @06:54:49.96 Lysis1.seek 100uL @06:55:10.99 delay 15 seconds @06:55:26.01 SPR.command "hold_off" @06:55:28.95 Waiting up to 1:00:00 for SPR to enter state 7 @07:26:01.45 SPR analysis completed @07:26:01.98 Ended log spr26jul3006h.spr.bz2 @07:26:02.00 SPR.command "stats_off" @07:26:03.41 Handoff.select :close @07:26:03.93 Gate.power :heat,:OFF @07:26:03.95 Gate.power :cartridge,:OFF @07:26:04.04 PinPort.in :unlocking @07:26:04.10 PinPort.seek :clear @07:26:04.93 Carousel.in :default @07:26:05.05 Carousel.home.seek 6 @07:26:05.38 Plunge.seek :locked @07:26:05.56 Archive.in :unlocking @07:26:05.71 Archive.seek :clear @07:26:05.77 Air.in :unlocking @07:26:05.95 Air.seek :clear @07:26:06.06 Lysis1.in :unlocking @07:26:06.22 Lysis1.seek :clear @07:26:06.29 Lysis2.in :unlocking @07:26:06.39 Lysis2.seek :clear @07:26:11.00 Gate.power :motor,:OFF @07:26:11.02 Gate.power :analytic1,:OFF @07:26:11.03 Gate.power :core,:OFF @07:26:11.05 Gate.power :main,:OFF @07:26:11.06 Safely stopped and ready to power off @07:26:11.66 Cartridge::None in detached from tina -- Not enough standard cartridges remain @09:22:15.45 Session Begun @09:22:20.44 -> ESP.ready1 @09:22:20.49 NoMethodError in preston -- undefined method `ready1' for ESP:Module @09:22:23.14 -> ESP.ready! @09:22:23.24 Gate.power :sampler,:OFF,limit to 3500mA @09:22:23.27 Gate.power :skimmer,:OFF,limit to 3500mA @09:22:23.30 Gate.power :main,:ON,limit to 5000mA @09:22:23.34 Gate.power :analytic1,:ON,limit to 4000mA @09:22:23.46 Powering core bus @09:22:25.84 SPR Module 1, FW Rev. B, FPGA Rev. C @09:22:26.23 Plunge.configure :default @09:22:26.34 SamplePump.configure :default @09:22:26.45 Toroid.configure :default @09:22:26.56 Carousel.configure :default @09:22:26.67 Abs.configure :default @09:22:26.78 PhaseSeparator.configure :default @09:22:26.89 Archive.configure :default @09:22:27.01 Air.configure :default @09:22:27.10 Lysis1.configure :default @09:22:27.21 Lysis2.configure :default @09:22:27.31 PinPort.configure :default @09:22:27.39 All elf controllers running firmware version 4.94 @09:22:27.42 Gate.power :motor,:ON,limit to 5000mA @09:22:27.70 Plunge.home.jog 0 @09:22:28.40 Lysis2.home.jog 0 @09:22:28.46 Lysis1.home.jog 0 @09:22:28.50 Archive.home.jog 0 @09:22:28.56 Toroid.home.jog 0 @09:22:28.67 PinPort.home.jog 0 @09:22:28.72 Air.home.jog 0 @09:22:28.76 Carousel.home.jog 0 @09:22:29.70 Plunge.in :homing @09:22:29.96 Plunge.jog 1501 @09:22:30.20 Archive.in :homing @09:22:30.31 Lysis1.in :homing @09:22:30.36 Lysis2.in :homing @09:22:30.42 Air.in :homing @09:22:30.46 Toroid.in :homing @09:22:30.52 PinPort.in :homing @09:22:30.82 Lysis1.jog 276 @09:22:30.85 Archive.jog 307 @09:22:30.91 Lysis2.jog 1621 @09:22:30.99 Air.jog 1434 @09:22:31.03 Toroid.jog 1363 @09:22:31.07 PinPort.jog 1236 @09:22:31.63 Plunge.setRawPosition! 50 @09:22:31.70 Plunge.seek :home @09:22:31.85 Lysis1.setRawPosition! 50 @09:22:31.93 Lysis1.seek :home @09:22:32.08 Archive.setRawPosition! 50 @09:22:32.10 Plunge.in :default @09:22:32.25 Archive.seek :home @09:22:32.37 Lysis1.in :default @09:22:32.58 Archive.in :default @09:22:32.60 Air.setRawPosition! 0 @09:22:32.75 Lysis2.setRawPosition! 50 @09:22:32.80 Air.seek :home @09:22:32.96 Lysis2.seek :home @09:22:33.13 Air.in :default @09:22:33.16 PinPort.setRawPosition! 3150 @09:22:33.35 PinPort.seek :home @09:22:33.89 Lysis2.in :default @09:22:35.85 Toroid.setRawPosition! 0 @09:22:35.93 Toroid.seek :home @09:22:36.05 Toroid.in :default @09:22:37.75 PinPort.in :default @09:22:38.11 Toroid.seek :clear @09:22:38.30 PinPort.in :unlocking @09:22:38.40 PinPort.seek :clear @09:22:41.76 Air.seek :clear @09:22:41.88 Lysis1.seek :clear @09:22:42.63 Lysis2.seek :clear @09:22:43.45 Archive.seek :clear @09:22:44.25 Carousel.in :default @09:22:44.41 Carousel.home.seek 6 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 6 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @3.76psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @09:23:27.67 -> SC.to 25 @09:23:27.72 Carousel.seek 25 Storage Carousel (SC) at 25 @09:25:02.27 -> SC.to 40 @09:25:02.33 Carousel.seek 40 Storage Carousel (SC) at 40 @09:25:39.55 -> SC.to 50 @09:25:39.60 Carousel.seek 50 Storage Carousel (SC) at 50 @09:27:33.97 -> exit @09:27:34.00 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:14:50.29 Session Begun @11:17:09.08 -> slot {1 => { Type: :archiveHiBiomass_bac, State: :missing}, 6 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul3006h.spr.bz2}, 12 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul3004h.spr.bz2}, 17 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul3003h.spr.bz2}, [52,22] => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}, 24 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul3002h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul3000h.spr.bz2}, 36 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul2923h.spr.bz2}, 42 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul2922h.spr.bz2}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul2920h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul2919h.spr.bz2}, 60 => { Type: :microSPR_phyto, State: :processed, lysed: 800, lysed2: 800, spr: spr26jul2918h.spr.bz2}} @11:17:33.90 -> slot1..60=>nil @11:17:33.93 SyntaxError in Tina -- compile error Tina:2: syntax error, unexpected tASSOC, expecting $end slot1..60=>nil ^ @11:18:03.20 -> slot 1..60=>nil {60..1 => nil} @11:18:08.16 -> slot {} @11:19:00.26 -> slot 53=>[:microSPR_phyto, :standard, {lysed:1000, lysed2:1000}] {53 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @11:19:14.16 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 800, lysed2: 800}} @11:21:51.26 -> Cart.aspectsOf :standard, extraDilutions:[1,1], lysed:950, lysed2:950 {extraDilutions: [1,1], lysed: 950, lysed2: 950} @11:22:24.21 -> slot 53=>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] {53 => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @11:23:01.64 -> to 53 @11:23:01.95 Carousel.seek 53 @11:23:02.94 Carousel.reconfigure :align @11:23:03.09 Lysis1.in :fast @11:23:03.19 Lysis1.seek :locked @11:23:05.87 Plunge.seek :locked @11:23:06.58 Air.in :fast @11:23:06.66 Air.seek :locked @11:23:07.77 Carousel.reconfigure :locked @11:23:07.97 Powering cartridge bus @11:23:10.63 Gate.setIR 1750,5000,0,6 @11:23:10.76 Gate.setIR 0,5000,0,6 @11:23:10.86 PuckHeater.configure :default @11:23:10.96 Gate.power :heat,:ON,limit to 5000mA @11:23:11.18 Mounted lysis Cartridge board#J59949 @11:23:11.27 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @11:23:33.28 -> PhytoSampler.prepareLGcart :full, :full, :full @11:23:33.30 Handoff.select :waste @11:23:33.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:23:40.45 Syringe tare is 51% of full scale at 23C (drifted -0%) @11:23:40.48 PinPort.in :locking @11:23:40.58 PinPort.seek :locked @11:23:41.45 Archive.in :fast @11:23:41.54 Lysis2.in :fast @11:23:41.60 Lysis1.seek :full @11:23:41.71 Archive.seek :full @11:23:41.84 Lysis2.seek :full Archive Syringe (ArS) full @11:24:22.62 -> Archive.empty @11:24:22.66 Archive.in :default @11:24:22.74 Archive.seek :empty Archive Syringe (ArS) empty @11:24:55.62 -> Lysis2.to 1000 @11:24:55.65 Lysis2.in :default @11:24:55.75 Lysis2.seek 1000uL Lysis Syringe #2 (LyS2) at 1000uL @11:25:17.03 -> Lysis1.to 1000 @11:25:17.08 Lysis1.in :default @11:25:17.16 Lysis1.seek 1000uL Lysis Syringe #1 (LyS1) at 1000uL @11:25:30.18 -> Cart.unlock @11:25:30.24 Gate.power :heat,:OFF @11:25:30.26 Gate.power :cartridge,:OFF @11:25:30.39 PinPort.in :unlocking @11:25:30.46 PinPort.seek :clear @11:25:31.31 Carousel.in :default @11:25:31.47 Carousel.home.seek 53 @11:25:31.82 Archive.in :unlocking @11:25:32.01 Archive.seek :clear @11:25:32.07 Air.in :unlocking @11:25:32.26 Air.seek :clear @11:25:32.93 Lysis2.in :unlocking @11:25:33.01 Lysis2.seek :clear @11:25:36.91 Lysis1.in :unlocking @11:25:37.08 Lysis1.seek :clear @11:25:37.21 Plunge.in :unlocking @11:25:37.33 Plunge.seek :clear @11:26:22.27 -> cart.aspects @11:26:22.32 NoMethodError in Tina -- undefined method `aspects' for :cart:Symbol @11:26:28.74 -> Cart.aspects {standard: {extraDilutions: [1,1], lysed: 950, lysed2: 950}} @11:26:36.32 -> slot {53 => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @11:26:58.36 -> start {runStandard} # @11:26:59.38 Toroid.seek :clear @11:26:59.59 Air.in :default @11:26:59.67 Air.seek :clear @11:26:59.79 Lysis1.in :default @11:26:59.88 Lysis1.seek :clear @11:27:00.00 Lysis2.in :default @11:27:00.07 Lysis2.seek :clear @11:27:00.19 Archive.in :default @11:27:00.28 Archive.seek :clear @11:27:00.38 Carousel.in :default @11:27:00.54 Carousel.home.seek 53 @11:27:00.83 Selecting Cartridge 53 @11:27:01.16 Carousel.seek 53 @11:27:01.53 Carousel.reconfigure :align @11:27:01.69 Lysis1.in :fast @11:27:01.78 Lysis1.seek :locked @11:27:04.44 Plunge.in :default @11:27:04.54 Plunge.seek :locked @11:27:04.72 Air.in :fast @11:27:04.82 Air.seek :locked @11:27:05.91 Carousel.reconfigure :locked @11:27:06.10 Powering cartridge bus @11:27:08.77 Gate.setIR 1750,5000,0,6 @11:27:08.90 Gate.setIR 0,5000,0,6 @11:27:08.99 PuckHeater.configure :default @11:27:09.08 Gate.power :heat,:ON,limit to 5000mA @11:27:09.32 Mounted lysis Cartridge board#J59949 @11:27:09.39 Using default cartridge heat loss curve for HeatingFunctionInCarousel @11:27:11.88 SPR.command "read_sequences" @11:27:12.12 -> exit @11:27:12.15 Session Ended @11:27:13.96 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AM1 (try 1 of 3) @11:27:17.54 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AM1 (try 2 of 3) @11:27:21.13 AnalyticModule::ReadTimeout in PROCESSING -- No response from SPR on AM1 @11:27:21.18 Thread::ChildDied in detached from Tina -- :PROCESSING @11:27:21.21 Waiting to Resume from Checkpoint ":PROCESSING" @11:27:40.97 Session Begun @11:27:59.74 -> sPR :sPR @11:28:01.49 -> SPR # @11:28:09.85 -> slot {53 => { Type: :microSPR_phyto, State: :processing, lysed: 950, lysed2: 950}} @11:28:36.23 -> slot 53=>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] {53 => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @11:28:57.58 -> Thread["detached from Tina].abort @11:28:57.61 SyntaxError in preston -- compile error preston:5: unterminated string meets end of file preston:5: syntax error, unexpected $end, expecting ']' Thread["detached from Tina].abort ^ @11:29:12.53 -> Thread["detached from Tina"].abort @11:29:12.58 Thread::Aborted in detached from Tina -- "Aborted by preston" # @11:29:12.63 Waiting to Resume from Checkpoint ":PROCESSING" @11:29:24.55 -> Thread["detached from Tina"].abort @11:29:24.60 Thread::Aborted in detached from Tina -- "Aborted by preston" # @11:29:24.66 Waiting to Resume from Checkpoint ":PROCESSING" @11:29:39.77 -> MainThread.exit @11:29:39.91 Handoff.select :close # @11:29:40.62 Session Ended @11:29:52.34EDT30-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @11:29:54.15 >BREAK< ESP3 Gateway v1.44 for core bus @11:29:54.18 >BREAK< ESP3 Gateway v1.44 for cartridge bus @11:29:58.41 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @11:29:59.09 Sea pressure (Abs) tare is 6% of full scale @11:30:02.36 Polling Can every 2:00 @11:30:02.41 Waiting for espclients... @11:30:03.12 Session Begun @11:30:10.74 -> slot {53 => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @11:30:18.25 -> Cart.aspects {standard: {extraDilutions: [1,1], lysed: 950, lysed2: 950}} @11:30:32.93 -> start {runStandard} # @11:30:33.32 Gate.power :sampler,:OFF,limit to 3500mA @11:30:33.34 Gate.power :skimmer,:OFF,limit to 3500mA @11:30:33.39 Gate.power :analytic1,:ON,limit to 4000mA @11:30:34.05 SPR Module 1, FW Rev. B, FPGA Rev. C @11:30:34.21 Plunge.configure :default @11:30:34.29 Plunge.hold @11:30:34.40 Plunge.configure :default @11:30:34.57 SamplePump.configure :default @11:30:34.76 Toroid.configure :default @11:30:34.85 Toroid.hold @11:30:34.96 Toroid.configure :default @11:30:35.12 Carousel.configure :default @11:30:35.24 Carousel.hold @11:30:35.35 Carousel.configure :default @11:30:35.52 Abs.configure :default @11:30:35.70 PhaseSeparator.configure :default @11:30:35.88 Archive.configure :default @11:30:35.96 Archive.hold @11:30:36.07 Archive.configure :default @11:30:36.23 Air.configure :default @11:30:36.32 Air.hold @11:30:36.42 Air.configure :default @11:30:36.56 Lysis1.configure :default @11:30:36.65 Lysis1.hold @11:30:36.74 Lysis1.configure :default @11:30:36.88 Lysis2.configure :default @11:30:36.97 Lysis2.hold @11:30:37.06 Lysis2.configure :default @11:30:37.26 PinPort.configure :default @11:30:37.35 PinPort.hold @11:30:37.45 PinPort.configure :default @11:30:37.53 All elf controllers running firmware version 4.94 @11:30:38.33 Toroid.seek :clear @11:30:38.53 Air.seek :clear @11:30:41.02 Lysis1.seek :clear @11:30:45.10 Lysis2.seek :clear @11:30:45.26 Archive.seek :clear @11:30:45.37 Carousel.in :default @11:30:45.53 Carousel.home.seek 53 @11:30:45.94 Selecting Cartridge 53 @11:30:46.45 Gate.power :heat,:OFF @11:30:46.48 Gate.power :cartridge,:OFF @11:30:46.61 Carousel.in :default @11:30:46.76 Carousel.home.seek 53 @11:30:47.62 Plunge.in :unlocking @11:30:47.72 Plunge.seek :clear @11:30:48.81 Carousel.seek 53 @11:30:49.12 Carousel.reconfigure :align @11:30:49.33 Lysis1.in :fast @11:30:49.41 Lysis1.seek :locked @11:30:52.06 Plunge.in :default @11:30:52.19 Plunge.seek :locked @11:30:52.34 Air.in :fast @11:30:52.42 Air.seek :locked @11:30:53.52 Carousel.reconfigure :locked @11:30:53.70 Powering cartridge bus @11:30:57.36 Gate.setIR 1750,5000,0,6 @11:30:57.50 Gate.setIR 0,5000,0,6 @11:30:57.59 PuckHeater.configure :default @11:30:57.70 Gate.power :heat,:ON,limit to 5000mA @11:30:57.95 Mounted lysis Cartridge board#J59949 @11:30:58.01 Using default cartridge heat loss curve for HeatingFunctionInCarousel @11:30:59.59 SPR.command "read_sequences" @11:31:00.42 SPR.command "stats_on" @11:31:00.60 SPR.command "start 0 1" @11:31:01.30 SPR.start /var/log/esp/spr26jul3011h#2.spr.bz2 @11:31:03.27 Waiting for baseline SPR stabilization @11:31:03.29 Handoff.select :waste @11:31:03.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:31:10.50 Syringe tare is 51% of full scale at 23C (drifted -0%) @11:31:10.53 PinPort.in :locking @11:31:10.64 PinPort.seek :locked @11:31:11.51 Archive.in :fast @11:31:11.61 Lysis2.in :fast @11:31:11.71 Lysis1.seek 950uL @11:31:11.80 Archive.seek :empty @11:31:12.50 Lysis2.seek 950uL @11:31:20.92 delayUntil "11:41:02.83" @11:32:08.47 -> exit @11:32:08.50 Session Ended @11:41:02.85 Lysis2.in :DAmix @11:41:02.96 Lysis1.in :DAmix @11:41:03.53 Lysis2.seek 700uL @11:41:03.59 Lysis1.seek 700uL @11:41:38.56 delay 5 seconds @11:41:43.58 Handoff.select SPR @11:41:44.09 SPR.command "start 2 14" @11:41:45.48 SPR stabilized @11:41:45.65 delay 20 seconds @11:42:05.68 SPR.command "start 2 0" @11:42:08.33 Waiting up to 11:00 for SPR to enter state 1 @11:44:05.99 SPR.command "hold_on" @11:44:07.90 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:44:08.49 Lysis2.seek 400uL @11:44:08.53 Lysis1.seek 400uL @11:44:50.51 delay 15 seconds @11:45:05.55 SPR.command "hold_off" @11:45:08.98 Waiting up to 1:00:00 for SPR to enter state 7 @12:01:23.60 SPRsummary:2.17RIU,30.3RIU,23.4RIU,35RIU after 1924s @12:17:28.48 delay 20 seconds @12:17:48.51 SPR.command "start 2 0" @12:17:51.58 Waiting up to 11:00 for SPR to enter state 1 @12:19:49.25 SPR.command "hold_on" @12:19:50.63 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:19:51.32 Lysis2.seek 250uL @12:19:51.38 Lysis1.seek 250uL @12:20:12.44 delay 15 seconds @12:20:27.46 SPR.command "hold_off" @12:20:30.48 Waiting up to 1:00:00 for SPR to enter state 7 @12:52:50.91 delay 20 seconds @12:53:10.94 SPR.command "start 2 0" @12:53:14.03 Waiting up to 11:00 for SPR to enter state 1 @12:55:11.69 SPR.command "hold_on" @12:55:13.08 SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:55:13.76 Lysis2.seek 100uL @12:55:13.82 Lysis1.seek 100uL @12:55:34.87 delay 15 seconds @12:55:49.89 SPR.command "hold_off" @12:55:53.45 Waiting up to 1:00:00 for SPR to enter state 7 @13:28:14.17 SPR analysis completed /home/esp/esp2/lib/schedule.rb:557:in `terminate!': Thread(0xb6923578): deadlock (fatal) from /home/esp/esp2/lib/log.rb:169:in `initialize' /home/esp/esp2/lib/schedule.rb:557:in `flush': Thread(0xb6923578): deadlock (fatal) from /home/esp/esp2/lib/log.rb:536:in `append' from /home/esp/esp2/lib/rmutex.rb:92:in `synchronize' from /home/esp/esp2/lib/log.rb:517:in `append' from /home/esp/esp2/lib/log.rb:561:in `recordObject' from /home/esp/esp2/lib/log.rb:580:in `recordException' from /home/esp/esp2/lib/log.rb:733:in `recordException' from /home/esp/esp2/lib/rmutex.rb:99:in `synchronize' from /home/esp/esp2/lib/log.rb:732:in `recordException' from /home/esp/esp2/lib/log.rb:313:in `recordException' from /home/esp/esp2/lib/esp.rb:91:in `mainThread' from /home/esp/esp2/lib/esp.rb:371 @13:55:00.85EDT30-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:55:02.67 >BREAK< ESP3 Gateway v1.44 for core bus @13:55:02.69 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:55:06.43 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:55:07.12 Sea pressure (Abs) tare is 6% of full scale @13:55:10.47 Polling Can every 2:00 @13:55:10.52 Waiting for espclients... @13:55:11.84 Session Begun @13:55:17.27 -> ESP.ready! @13:55:17.48 Gate.power :sampler,:OFF,limit to 3500mA @13:55:17.50 Gate.power :skimmer,:OFF,limit to 3500mA @13:55:17.54 Gate.power :analytic1,:ON,limit to 4000mA @13:55:18.27 Plunge.configure :default @13:55:18.37 Plunge.hold @13:55:18.47 Plunge.configure :default @13:55:18.64 SamplePump.configure :default @13:55:18.81 Toroid.configure :default @13:55:18.90 Toroid.hold @13:55:19.01 Toroid.configure :default @13:55:19.24 Carousel.configure :default @13:55:19.33 Carousel.hold @13:55:19.44 Carousel.configure :default @13:55:19.88 Abs.configure :default @13:55:20.08 PhaseSeparator.configure :default @13:55:20.24 Archive.configure :default @13:55:20.33 Archive.hold @13:55:20.44 Archive.configure :default @13:55:20.61 Air.configure :default @13:55:20.70 Air.hold @13:55:20.80 Air.configure :default @13:55:20.94 Lysis1.configure :default @13:55:21.07 Lysis1.hold @13:55:21.20 Lysis1.configure :default @13:55:21.63 Lysis2.configure :default @13:55:21.71 Lysis2.hold @13:55:21.82 Lysis2.configure :default @13:55:22.02 PinPort.configure :default @13:55:22.11 PinPort.hold @13:55:22.22 PinPort.configure :default @13:55:22.30 All elf controllers running firmware version 4.94 @13:55:23.06 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @13:55:23.09 AnalyticModule::Unknown in preston -- SPR @13:55:23.98 Toroid.seek :clear @13:55:24.20 Air.seek :clear @13:55:26.69 Lysis1.seek :clear @13:55:44.48 Lysis2.seek :clear @13:56:02.22 Archive.seek :clear @13:56:15.76 Archive.hold @13:56:16.37 UserAbort in preston -- Archive Syringe (ArS) seek to clear Aborted! @13:56:19.45 -> ESP.reready! @13:56:19.51 Plunge.in :default @13:56:19.57 Toroid.in :default @13:56:19.72 Carousel.in :default @13:56:19.82 Archive.in :default @13:56:19.95 Air.in :default @13:56:20.12 Toroid.home.jog 0 @13:56:20.16 Lysis1.in :default @13:56:20.69 Lysis2.in :default @13:56:20.74 Plunge.home.jog 0 @13:56:20.86 Carousel.home.jog 0 @13:56:20.89 PinPort.in :default @13:56:20.99 Archive.home.jog 0 @13:56:21.12 Air.home.jog 0 @13:56:21.57 PinPort.home.jog 0 @13:56:21.69 Lysis2.home.jog 0 @13:56:21.75 Lysis1.home.jog 0 @13:56:22.56 Toroid.in :homing @13:56:22.78 Toroid.jog 1350 @13:56:22.87 PinPort.in :homing @13:56:22.92 Lysis1.in :homing @13:56:22.96 Archive.in :homing @13:56:23.00 Lysis2.in :homing @13:56:23.05 Air.in :homing @13:56:23.10 Plunge.in :homing @13:56:23.48 PinPort.jog 3150 @13:56:23.57 Lysis1.jog 1602 @13:56:23.62 Archive.jog 2841 @13:56:23.66 Lysis2.jog 1621 @13:56:23.73 Air.jog 1429 @13:56:23.76 Plunge.jog 1478 @13:56:25.13 Lysis1.setRawPosition! 50 @13:56:25.27 Lysis1.seek :home @13:56:25.40 Lysis2.setRawPosition! 50 @13:56:25.47 Air.setRawPosition! 0 @13:56:25.52 Plunge.setRawPosition! 50 @13:56:25.70 Lysis2.seek :home @13:56:25.74 Air.seek :home @13:56:25.89 Plunge.seek :home @13:56:26.11 Air.in :default @13:56:26.18 Archive.setRawPosition! 50 @13:56:26.22 Lysis1.in :default @13:56:26.44 Archive.seek :home @13:56:26.58 Lysis2.in :default @13:56:26.61 Plunge.in :default @13:56:26.79 Archive.in :default @13:56:27.68 Toroid.setRawPosition! 0 @13:56:27.76 Toroid.seek :home @13:56:27.87 Toroid.in :default @13:56:28.20 PinPort.setRawPosition! 3150 @13:56:28.28 PinPort.seek :home @13:56:32.66 PinPort.in :default @13:56:33.02 Toroid.seek :clear @13:56:33.26 PinPort.in :unlocking @13:56:33.34 PinPort.seek :clear @13:56:36.76 Air.seek :clear @13:56:36.90 Lysis1.seek :clear @13:56:37.66 Lysis2.seek :clear @13:56:38.48 Archive.seek :clear @13:56:39.28 Carousel.in :default @13:56:39.44 Carousel.home.seek 53 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 53 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @3.76psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:56:41.06 -> ESP.shutdown @13:56:41.22 Intake.close @13:56:41.32 Exhaust.close @13:56:41.40 Bypass.close @13:56:41.74 Gate.power :heat,:OFF @13:56:41.76 Gate.power :cartridge,:OFF @13:56:41.80 Gate.power :motor,:OFF @13:56:41.83 Gate.power :analytic1,:OFF @13:56:41.86 Gate.power :core,:OFF @13:56:41.90 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @13:56:46.91 -> exit @13:56:46.93 Session Ended @01:30:23.46EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @01:30:25.43 >BREAK< ESP3 Gateway v1.44 for core bus @01:30:25.46 >BREAK< ESP3 Gateway v1.44 for cartridge bus @01:30:29.27 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @01:30:29.97 Sea pressure (Abs) tare is 6% of full scale @01:30:33.45 Polling Can every 2:00 @01:30:33.50 Waiting for espclients... @01:30:34.58 Session Begun @01:30:34.70 -> ESP::Mode :real @01:31:43.88 -> slots {[42,10] => { Type: :microSPR_phyto, State: :reserved, lysed: 1800, lysed2: 1800, was: :standard}, 55 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @01:32:03.27 -> Cart.aspects {standard: {extraDilutions: [1,1], lysed: 950, lysed2: 950}} @01:32:21.51 -> Cart.aspectsOf :standard, extraDilutions:[],lysed:1100, lysed2:1100 {extraDilutions: [], lysed: 1100, lysed2: 1100} @01:32:42.87 -> start{runStandard} # @01:32:43.23 Gate.power :sampler,:OFF,limit to 3500mA @01:32:43.31 Gate.power :skimmer,:OFF,limit to 3500mA @01:32:43.34 Gate.power :analytic1,:ON,limit to 4000mA @01:32:44.08 Plunge.configure :default @01:32:44.17 Plunge.hold @01:32:44.27 Plunge.configure :default @01:32:44.45 SamplePump.configure :default @01:32:44.64 Toroid.configure :default @01:32:44.71 SPR Module 1, FW Rev. B, FPGA Rev. C @01:32:44.78 Toroid.hold @01:32:44.88 Toroid.configure :default @01:32:45.13 Carousel.configure :default @01:32:45.28 Carousel.hold @01:32:45.41 Carousel.configure :default @01:32:45.59 Abs.configure :default @01:32:45.76 PhaseSeparator.configure :default @01:32:45.93 Archive.configure :default @01:32:46.02 Archive.hold @01:32:46.13 Archive.configure :default @01:32:46.30 Air.configure :default @01:32:46.38 Air.hold @01:32:46.49 Air.configure :default @01:32:46.62 Lysis1.configure :default @01:32:46.70 Lysis1.hold @01:32:46.79 Lysis1.configure :default @01:32:46.94 Lysis2.configure :default @01:32:47.02 Lysis2.hold @01:32:47.13 Lysis2.configure :default @01:32:47.35 PinPort.configure :default @01:32:47.44 PinPort.hold @01:32:47.55 PinPort.configure :default @01:32:47.64 All elf controllers running firmware version 4.94 @01:32:48.58 Toroid.seek :clear @01:32:48.79 Air.seek :clear @01:32:48.91 Lysis1.seek :clear @01:32:49.04 Lysis2.seek :clear @01:32:49.15 Archive.seek :clear @01:32:49.31 Carousel.in :default @01:32:49.46 Carousel.home.seek 55 @01:32:50.14 Selecting Cartridge 55 @01:32:50.48 Carousel.seek 55 @01:32:50.78 Carousel.reconfigure :align @01:32:50.93 Lysis1.in :fast @01:32:51.02 Lysis1.seek :locked @01:32:53.68 Plunge.seek :locked @01:32:53.88 Air.in :fast @01:32:53.98 Air.seek :locked @01:32:55.08 Carousel.reconfigure :locked @01:32:55.30 Powering cartridge bus @01:32:57.97 Gate.setIR 1750,5000,0,6 @01:32:58.10 Gate.setIR 0,5000,0,6 @01:32:58.19 PuckHeater.configure :default @01:32:58.29 Gate.power :heat,:ON,limit to 5000mA @01:32:58.54 Mounted lysis Cartridge board#J59949 @01:32:58.60 Using default cartridge heat loss curve for HeatingFunctionInCarousel @01:33:00.19 SPR.command "read_sequences" @01:33:00.68 SPR.command "stats_on" @01:33:00.87 SPR.command "start 0 1" @01:33:01.56 SPR.start /var/log/esp/spr26jul3101h.spr.bz2 @01:33:03.53 Waiting for baseline SPR stabilization @01:33:03.55 Handoff.select :waste @01:33:04.15 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:33:10.79 Syringe tare is 51% of full scale at 22C (drifted -0%) @01:33:10.84 PinPort.in :locking @01:33:10.92 PinPort.seek :locked @01:33:11.79 Archive.in :fast @01:33:11.90 Lysis2.in :fast @01:33:11.99 Lysis1.seek 1100uL @01:33:12.06 Archive.seek :empty @01:33:12.24 Lysis2.seek 1100uL @01:33:20.56 delayUntil "01:43:03.14" @01:43:03.15 Lysis2.in :DAmix @01:43:03.34 Lysis1.in :DAmix @01:43:03.87 Lysis2.seek 850uL @01:43:03.96 Lysis1.seek 850uL @01:43:38.95 delay 5 seconds @01:43:43.98 Handoff.select SPR @01:43:44.50 SPR.command "start 2 14" @01:43:45.77 SPR stabilized @01:43:45.96 delay 20 seconds @01:44:05.99 SPR.command "start 2 0" @01:44:09.16 Waiting up to 11:00 for SPR to enter state 1 @01:46:07.25 SPR.command "hold_on" @01:46:08.61 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:46:09.27 Lysis2.seek 550uL @01:46:09.34 Lysis1.seek 550uL @01:46:51.35 delay 15 seconds @01:47:06.37 SPR.command "hold_off" @01:47:09.29 Waiting up to 1:00:00 for SPR to enter state 7 @02:03:24.89 SPRsummary:4.89e+09RIU,1e+09RIU,14.7RIU,1e+09RIU after 10801s @02:17:41.83 SPR analysis completed @02:17:45.07 Ended log spr26jul3101h.spr.bz2 @02:17:45.09 SPR.command "stats_off" @02:18:41.05 -> threads [#, #, #, #] @02:20:02.92 -> slot {[42,10] => { Type: :microSPR_phyto, State: :reserved, lysed: 1800, lysed2: 1800, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: spr26jul3101h.spr.bz2}} @02:23:03.80 -> exit @02:23:03.83 Session Ended @02:23:18.43 Session Begun @02:23:25.45 -> ESP.main.exit @02:23:25.58 Intake.close @02:23:25.68 Exhaust.close @02:23:25.73 Bypass.close @02:23:25.77 Handoff.select :close # @02:23:26.59 Session Ended @02:23:36.59EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @02:23:38.30 >BREAK< ESP3 Gateway v1.44 for core bus @02:23:38.33 >BREAK< ESP3 Gateway v1.44 for cartridge bus @02:23:41.99 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @02:23:42.66 Sea pressure (Abs) tare is 6% of full scale @02:23:46.20 Polling Can every 2:00 @02:23:46.70 Clients may connect on TCP port 9999 @02:23:46.71 Waiting for espclients... @02:23:56.56 Session Begun @02:24:07.46 -> slot {[42,10] => { Type: :microSPR_phyto, State: :reserved, lysed: 1800, lysed2: 1800, was: :standard}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr26jul3101h.spr.bz2"}} @02:24:24.72 -> Cart.release 56 0 @02:24:32.53 -> Cart.release 42 1 @02:24:36.26 -> slot {10 => { Type: :microSPR_phyto, State: :reserved, lysed: 1800, lysed2: 1800, was: :standard}, 42 => { Type: :microSPR_phyto, State: :standard, lysed: 1800, lysed2: 1800}, 55 => { Type: :microSPR_phyto, State: :processed, lysed: 1100, lysed2: 1100, spr: "spr26jul3101h.spr.bz2"}} @02:28:19.20 -> start{runStandard} # @02:28:19.61 Gate.power :sampler,:OFF,limit to 3500mA @02:28:19.64 Gate.power :skimmer,:OFF,limit to 3500mA @02:28:19.68 Gate.power :analytic1,:ON,limit to 4000mA @02:28:20.40 Plunge.configure :default @02:28:20.48 Plunge.hold @02:28:20.58 Plunge.configure :default @02:28:20.76 SPR Module 1, FW Rev. B, FPGA Rev. C @02:28:20.84 SamplePump.configure :default @02:28:21.02 Toroid.configure :default @02:28:21.11 Toroid.hold @02:28:21.20 Toroid.configure :default @02:28:21.40 Carousel.configure :default @02:28:21.54 Carousel.hold @02:28:21.72 Carousel.configure :default @02:28:21.89 Abs.configure :default @02:28:22.06 PhaseSeparator.configure :default @02:28:22.22 Archive.configure :default @02:28:22.31 Archive.hold @02:28:22.40 Archive.configure :default @02:28:22.57 Air.configure :default @02:28:22.66 Air.hold @02:28:22.76 Air.configure :default @02:28:22.90 Lysis1.configure :default @02:28:22.99 Lysis1.hold @02:28:23.09 Lysis1.configure :default @02:28:23.23 Lysis2.configure :default @02:28:23.36 Lysis2.hold @02:28:23.46 Lysis2.configure :default @02:28:23.64 PinPort.configure :default @02:28:23.73 PinPort.hold @02:28:23.84 PinPort.configure :default @02:28:23.92 All elf controllers running firmware version 4.94 @02:28:24.67 Toroid.seek :clear @02:28:25.01 Air.seek :clear @02:28:26.28 -> exit @02:28:26.30 Session Ended @02:28:27.51 Lysis1.seek :clear @02:28:33.82 Session Begun @02:28:41.35 Lysis2.seek :clear @02:28:41.81 -> showlog @02:28:55.16 Archive.seek :clear @02:29:13.76 Carousel.in :default @02:29:14.29 Carousel.home.seek 55 @02:29:14.75 Selecting Cartridge 42 @02:29:14.93 Gate.power :heat,:OFF @02:29:14.97 Gate.power :cartridge,:OFF @02:29:15.18 PinPort.in :unlocking @02:29:15.30 PinPort.seek :clear @02:29:16.16 Carousel.in :default @02:29:16.32 Carousel.home.seek 55 @02:29:16.97 Plunge.in :unlocking @02:29:17.06 Plunge.seek :clear @02:29:18.09 Carousel.seek 42 @02:29:21.15 Carousel.reconfigure :align @02:29:21.38 Lysis1.in :fast @02:29:21.53 Lysis1.seek :locked @02:29:24.19 Plunge.in :default @02:29:24.32 Plunge.seek :locked @02:29:24.47 Air.in :fast @02:29:24.58 Air.seek :locked @02:29:25.70 Carousel.reconfigure :locked @02:29:25.89 Powering cartridge bus @02:29:29.56 Gate.setIR 1750,5000,0,6 @02:29:29.69 Gate.setIR 0,5000,0,6 @02:29:29.78 PuckHeater.configure :default @02:29:29.88 Gate.power :heat,:ON,limit to 5000mA @02:29:30.13 Mounted lysis Cartridge board#J59993 @02:29:30.17 Using default cartridge heat loss curve for HeatingFunctionInCarousel @02:29:31.75 SPR.command "read_sequences" @02:29:32.55 SPR.command "stats_on" @02:29:32.72 SPR.command "start 0 1" @02:29:33.43 SPR.start /var/log/esp/spr26jul3102h.spr.bz2 @02:29:35.40 Waiting for baseline SPR stabilization @02:29:35.42 Handoff.select :waste @02:29:35.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:29:43.11 Syringe tare is 51% of full scale at 22C (drifted -0%) @02:29:43.14 PinPort.in :locking @02:29:43.37 PinPort.seek :locked @02:29:44.22 Archive.in :fast @02:29:44.28 Lysis2.in :fast @02:29:44.43 Lysis1.seek 1800uL @02:29:44.53 Archive.seek :empty @02:29:44.76 Lysis2.seek 1800uL @02:29:53.11 delayUntil "02:39:35.01" @02:30:22.83 -> exit @02:30:22.85 Session Ended @02:39:35.03 Lysis2.in :DAmix @02:39:35.14 Lysis1.in :DAmix @02:39:35.69 Lysis2.seek 1550uL @02:39:35.75 Lysis1.seek 1550uL @02:40:10.70 delay 5 seconds @02:40:15.72 Handoff.select SPR @02:40:16.22 SPR.command "start 2 14" @02:40:16.72 SPR stabilized @02:40:16.96 delay 20 seconds @02:40:36.98 SPR.command "start 2 0" @02:40:39.54 Waiting up to 11:00 for SPR to enter state 1 @02:42:37.22 SPR.command "hold_on" @02:42:38.59 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @02:42:39.19 Lysis2.seek 1250uL @02:42:39.31 Lysis1.seek 1250uL @02:43:21.18 delay 15 seconds @02:43:36.20 SPR.command "hold_off" @02:43:39.80 Waiting up to 1:00:00 for SPR to enter state 7 @02:46:17.99 Session Begun @02:46:26.35 -> showlog 10 @02:46:29.05 -> exit @02:46:29.07 Session Ended @02:59:55.82 SPRsummary:3.08e+09RIU,1e+09RIU,1.49RIU,1e+09RIU after 13635s @03:14:12.89 delay 20 seconds @03:14:32.91 SPR.command "start 2 0" @03:14:36.53 Waiting up to 11:00 for SPR to enter state 1 @03:16:34.63 SPR.command "hold_on" @03:16:36.53 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @03:16:37.15 Lysis2.seek 1100uL @03:16:37.20 Lysis1.seek 1100uL @03:16:58.29 delay 15 seconds @03:17:13.32 SPR.command "hold_off" @03:17:15.84 Waiting up to 1:00:00 for SPR to enter state 7 @03:47:47.60 delay 20 seconds @03:48:07.63 SPR.command "start 2 0" @03:48:11.23 Waiting up to 11:00 for SPR to enter state 1 @03:50:09.34 SPR.command "hold_on" @03:50:11.22 SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @03:50:11.83 Lysis2.seek 950uL @03:50:11.90 Lysis1.seek 950uL @03:50:32.96 delay 15 seconds @03:50:48.00 SPR.command "hold_off" @03:50:50.52 Waiting up to 1:00:00 for SPR to enter state 7 @04:21:22.14 SPR analysis completed @04:21:46.01 Ended log spr26jul3102h.spr.bz2 @04:21:46.02 SPR.command "stats_off" @09:48:21.58 Session Begun @09:49:48.05 -> Cart.unlock @09:49:48.11 Gate.power :heat,:OFF @09:49:48.12 Gate.power :cartridge,:OFF @09:49:48.26 PinPort.in :unlocking @09:49:48.35 PinPort.seek :clear @09:49:49.21 Carousel.in :default @09:49:49.39 Carousel.home.seek 42 @09:49:49.78 Archive.in :unlocking @09:49:49.89 Archive.seek :clear @09:49:50.04 Air.in :unlocking @09:49:50.21 Air.seek :clear @09:49:50.33 Lysis2.in :unlocking @09:49:50.46 Lysis2.seek :clear @09:49:54.67 Lysis1.in :unlocking @09:49:54.83 Lysis1.seek :clear @09:49:54.88 Plunge.in :unlocking @09:49:55.02 Plunge.seek :clear @09:50:44.15 -> SC.to 50 @09:50:44.22 Carousel.seek 50 Storage Carousel (SC) at 50 @09:51:09.87 -> SC.to 20 @09:51:09.92 Carousel.seek 20 Storage Carousel (SC) at 20 @09:51:38.18 -> slots 1..60=>nil {60..1 => nil} @09:52:01.35 -> ESP.shutdown @09:52:01.48 Intake.close @09:52:01.54 Exhaust.close @09:52:01.65 Bypass.close @09:52:01.73 Handoff.select :close @09:52:02.50 Gate.power :motor,:OFF @09:52:02.52 Gate.power :analytic1,:OFF @09:52:02.61 Gate.power :core,:OFF @09:52:02.65 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @09:52:04.36 -> exit @09:52:04.38 Session Ended @13:51:35.11EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:51:36.86 >BREAK< ESP3 Gateway v1.44 for core bus @13:51:36.88 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:51:40.48 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:51:41.15 Sea pressure (Abs) tare is 6% of full scale @13:51:44.73 Polling Can every 2:00 @13:51:44.77 Waiting for espclients... @13:51:46.02 Session Begun @13:51:50.45 -> slot {[40,20] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [50,45] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 55 => { Type: :lyseHold_phyto, State: :dry}} @13:52:16.95 -> start {fire :lyseHold_phyto} # @13:52:17.31 Gate.power :analytic1,:ON,limit to 4000mA @13:52:17.33 Gate.power :analytic2,:ON,limit to 4000mA @13:52:17.38 Gate.power :analytic3,:ON,limit to 4000mA @13:52:17.99 SPR Module 1, FW Rev. B, FPGA Rev. C @13:52:18.19 Plunge.configure :default @13:52:18.28 Plunge.hold @13:52:18.39 Plunge.configure :default @13:52:18.55 SamplePump.configure :default @13:52:18.71 Toroid.configure :default @13:52:18.80 Toroid.hold @13:52:18.90 Toroid.configure :default @13:52:19.07 Carousel.configure :default @13:52:19.16 Carousel.hold @13:52:19.26 Carousel.configure :default @13:52:19.42 Abs.configure :default @13:52:19.58 PhaseSeparator.configure :default @13:52:19.78 Archive.configure :default @13:52:19.87 Archive.hold @13:52:19.98 Archive.configure :default @13:52:20.14 Air.configure :default @13:52:20.23 Air.hold @13:52:20.32 Air.configure :default @13:52:20.47 Lysis1.configure :default @13:52:20.56 Lysis1.hold @13:52:20.66 Lysis1.configure :default @13:52:20.80 Lysis2.configure :default @13:52:20.89 Lysis2.hold @13:52:20.99 Lysis2.configure :default @13:52:21.15 PinPort.configure :default @13:52:21.23 PinPort.hold @13:52:21.33 PinPort.configure :default @13:52:21.40 All elf controllers running firmware version 4.94 @13:52:22.25 Toroid.seek :clear @13:52:22.47 Air.seek :clear @13:52:27.23 Lysis1.seek :clear @13:52:29.08 -> exit @13:52:29.11 Session Ended @13:52:31.31 Lysis2.seek :clear @13:52:31.40 Archive.seek :clear @13:52:31.98 Carousel.in :default @13:52:32.10 Carousel.home.seek 55 @13:52:32.45 Selecting Cartridge 55 @13:52:32.56 Gate.power :heat,:OFF @13:52:32.57 Gate.power :cartridge,:OFF @13:52:32.65 Carousel.in :default @13:52:32.78 Carousel.home.seek 55 @13:52:33.41 Plunge.in :unlocking @13:52:33.47 Plunge.seek :clear @13:52:34.46 Carousel.seek 55 @13:52:34.73 Carousel.reconfigure :align @13:52:34.86 Lysis1.in :fast @13:52:34.92 Lysis1.seek :locked @13:52:37.55 Plunge.in :default @13:52:37.65 Plunge.seek :locked @13:52:37.82 Air.in :fast @13:52:37.87 Air.seek :locked @13:52:38.94 Carousel.reconfigure :locked @13:52:39.10 Powering cartridge bus @13:52:42.78 Gate.setIR 1750,5000,0,6 @13:52:42.92 Gate.setIR 0,5000,0,6 @13:52:42.98 PuckHeater.configure :default @13:52:43.05 Gate.power :heat,:ON,limit to 5000mA @13:52:43.27 Mounted lysis Cartridge board#J59943 @13:52:43.30 Using default cartridge heat loss curve for HeatingFunctionInCarousel @13:52:43.61 Preparing to filter lyseHold_phyto cartridge in slot 55 @13:52:43.89 Air.in :default @13:52:44.01 Air.seek :sealed @13:52:44.10 Priming sample loop w/200ml, bypass w/10ml @13:52:44.12 SamplePump.setPosition! 0ml @13:52:44.16 Exhaust.close @13:52:44.25 Bypass.open @13:52:46.56 Intake.open @13:52:48.08 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:52:50.70 Vacuum tare is 54% of full scale at 24C (drifted 2%) @13:52:50.86 Abs.in :default @13:52:50.91 Abs.hold @13:52:51.64 SamplePump.in :start @13:52:51.70 SamplePump.seek 10ml,1:10 @13:52:52.33 SamplePump.configure :prime @13:53:05.23 Abs.stop @13:53:05.28 SamplePump.setPosition! 0ml @13:53:05.34 Exhaust.open @13:53:07.57 Bypass.close @13:53:09.94 Abs.in :default @13:53:10.00 Abs.hold @13:53:10.34 SamplePump.in :start @13:53:10.40 SamplePump.seek 200ml,13:50 @13:53:11.03 SamplePump.configure :prime @13:57:33.20 Abs.stop @13:57:33.28 Bypass.open @13:57:34.81 Intake.close @13:57:36.35 Toroid.in :hold @13:57:36.40 Toroid.seek :crack @13:57:38.87 Toroid.seek :cartridge @13:57:40.99 Toroid.in :default @13:57:41.04 Toroid.seek :clear @13:57:45.01 Pressurizing cartridge to 14psi above 16.1psia @13:57:45.03 SamplePump.setPosition! 0ml @13:57:45.08 Intake.open @13:57:46.52 Bypass.close @13:57:48.05 Exhaust.close @13:57:49.71 Abs.in :default @13:57:49.78 Abs.hold @13:57:50.02 SamplePump.in :charge @13:57:50.09 SamplePump.seek 2.3ml,38 seconds @13:58:04.37 Abs.stop @13:58:04.44 Intake.close @13:58:06.01 Pressurized cartridge with 0.848ml to 14.2psi @13:58:06.03 Isolated @29.5psia (13.4psi) @13:58:06.05 Verifying cartridge holds at least 0.65..0.85 of 29.5psia @13:58:06.09 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:58:10.68 Cartridge pressure fell 2% to 28.8psia @13:58:11.29 Sampling 100.0ml @13:58:11.31 SamplePump.setPosition! 0ml @13:58:11.37 Exhaust.open @13:58:12.88 Intake.open @13:58:14.56 Abs.in :default @13:58:14.61 Abs.hold @13:58:14.94 SamplePump.in :start @13:58:15.01 SamplePump.seek 100ml,6:04 @13:58:15.63 SamplePump.configure :sample @13:58:18.04 Cartridge 55 filtering 2/100ml at 0.74ml/s with 11.8psi vacuum @13:59:18.09 Cartridge 55 filtering 47/100ml at 0.74ml/s with 6.0psi vacuum 4.3m deep (21.0psia) @14:00:18.10 Cartridge 55 filtering 91/100ml at 0.74ml/s with 11.5psi vacuum @14:00:30.33 Abs.stop @14:00:30.41 Intake.close @14:00:30.45 Exhaust.close @14:00:30.70 Toroid.in :hold @14:00:30.76 Toroid.seek :bypass @14:00:34.35 Toroid.in :default @14:00:34.40 Toroid.seek :clear @14:00:37.98 Sampled 100.0ml @14:00:38.49 Handoff.select :waste @14:00:38.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:00:45.62 Syringe tare is 51% of full scale at 25C (drifted -0%) @14:00:45.64 PinPort.in :locking @14:00:45.69 PinPort.seek :locked @14:00:46.52 Archive.in :fast @14:00:46.62 Lysis2.in :fast @14:00:46.67 Lysis1.seek 1250uL @14:00:46.70 Archive.seek :empty @14:00:46.84 Lysis2.seek 950uL @14:00:55.20 Evacuating puck with filter bubble point of 20psi @14:00:55.23 PinPort.seek :locked @14:00:55.31 Handoff.select :waste @14:00:55.33 Plunge.seek :puckBottom @14:00:56.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:01:03.32 Air syringe pumping up to 1 strokes to reach 4psi @14:01:03.34 Air stroke #1 of 1 @14:01:03.42 Air.in :pressurize @14:01:03.47 Air.seek :pressurized @14:01:08.72 -0.2psi after pressurize @14:01:08.74 Air.in :compress @14:01:08.79 Air.seek :evac @14:01:15.40 0.9psi after compress @14:01:25.99 0.1psi after compress (stable) @14:01:26.01 Air.in :default @14:01:26.06 Air.hold @14:01:26.27 at -0.0psi Opened to waste @14:01:26.28 Handoff.select :blocked @14:01:26.77 Plunge.seek :puckTop @14:01:27.64 Air syringe pumping up to 8 strokes to reach 20psi @14:01:27.65 Air stroke #1 of 8 @14:01:27.75 Air.in :compress @14:01:27.84 Air.seek :evac @14:01:27.97 1.0psi after compress @14:01:38.97 1.6psi after compress (stable) @14:01:39.00 Air.in :gulp @14:01:39.05 Air.seek :topVent @14:01:45.50 1.6psi after gulp @14:01:51.09 1.7psi after gulp (stable) @14:01:51.10 Air stroke #2 of 8 @14:01:51.19 Air.in :pressurize @14:01:51.24 Air.seek :pressurized @14:01:57.62 1.8psi after pressurize @14:01:57.64 Air.in :compress @14:01:57.69 Air.seek :evac @14:02:04.30 13.1psi after compress @14:02:15.39 15.2psi after compress (stable) @14:02:15.41 Air.in :gulp @14:02:15.46 Air.seek :topVent @14:02:21.92 15.1psi after gulp @14:02:27.52 15.4psi after gulp (stable) @14:02:27.53 Air stroke #3 of 8 @14:02:27.61 Air.in :pressurize @14:02:27.66 Air.seek :pressurized @14:02:34.04 15.3psi after pressurize @14:02:34.06 Air.in :compress @14:02:34.11 Air.seek :evac @14:02:39.57 Reached 20.3 of 20.0psi after pumping 5234uL (2.95 strokes) @14:02:39.69 Air.in :default @14:02:39.75 Air.hold @14:02:39.86 Plunge.seek :puckBottom @14:02:40.73 delay 3 seconds @14:02:43.76 Handoff.select :waste @14:02:44.27 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:02:54.86 delay 1:00 @14:03:54.89 Handoff.select :blocked @14:03:55.38 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:04:17.97 Handoff.select :waste @14:04:18.46 0.1psi at evacEnd @14:04:18.48 Plunge.seek :pTop @14:04:19.34 delay 5 seconds @14:04:24.37 Air.in :glide @14:04:24.42 Air.seek :fullVent @14:04:27.02 delay 2 seconds @14:04:29.05 Plunge.seek :pBottom @14:04:29.90 Seawater evacuated, Pressure above/below filter released. @14:04:29.92 Plunge.seek :locked @14:04:31.21 Handoff.select :waste @14:04:31.31 Lysis1.in :slow @14:04:31.44 Lysis1.seek 950uL @14:04:45.51 delay 5 seconds @14:04:50.53 Handoff.select :blocked @14:04:51.04 Plunge.seek :puckTop @14:04:51.99 Lysis1.seek 350uL @14:05:19.99 delay 3 seconds @14:05:23.08 Air.in mix with 2.79934 parts Lysis1 @14:05:23.71 Air.seek :evac @14:05:23.79 Lysis1.seek 190uL @14:05:31.47 Air.in :fast @14:05:31.53 Air.seek :full @14:05:38.03 Lysis1.seek :empty @14:05:47.13 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @18.8psi @14:05:47.15 Plunge.seek :puckBottom @14:05:48.14 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @15.7psi @14:05:48.16 Polling Can every 2:00 @14:05:48.70 PuckHeater.configure :default #ambient 24.6C @14:05:48.75 PuckHeater.seek 81.6C @14:06:35.99 PuckHeater.hold 2:00,81.6C @14:08:35.92 Polling Can every 2:00 @14:08:36.47 PuckHeater.configure :default #ambient 24.6C @14:08:36.52 PuckHeater.seek 80.5C @14:08:36.71 PuckHeater.hold 2:00,80.5C @14:10:36.66 Polling Can every 2:00 @14:10:37.20 PuckHeater.configure :default #ambient 24.7C @14:10:37.26 PuckHeater.hold 8:00,79.4C @14:18:36.62 Lysate cooked @16.0psi @14:18:36.64 Plunge.seek :puckTop @14:18:37.60 Air syringe pumping up to 5 strokes to reach 40psi @14:18:37.62 Air stroke #1 of 5 @14:18:37.70 Air.in :pressurize @14:18:37.79 Air.seek :pressurized @14:18:44.17 15.7psi after pressurize @14:18:44.20 Air.in :compress @14:18:44.25 Air.seek :evac @14:18:49.45 Reached 40.9 of 40.0psi after pumping 1692uL (0.95 strokes) @14:18:49.47 Air.in :default @14:18:49.52 Air.hold @14:18:49.60 delay 2 seconds @14:18:51.63 Air.in :fast @14:18:51.67 Air.seek :full @14:18:57.88 Plunge.seek :puckBottom @14:18:58.82 Lysis1.seek 138uL @14:19:05.37 delay 2 seconds @14:19:07.40 Plunge.seek :puckTop @14:19:08.26 Lysis1.seek :empty @14:19:14.82 Plunge.seek :puckBottom @14:19:15.95 Lysis1.in :extract @14:19:16.05 Lysis1.seek 675uL,nil @14:19:19.56 Air.in :pressurize @14:19:19.63 Air.seek :pressurized @14:19:26.26 10.5psi after pressurize @14:19:26.28 Air.in :compressLysate @14:19:26.34 Air.seek :evac @14:19:45.04 Reached 15.9 of 15.5psi after pumping 1665uL (0.94 strokes) @14:19:45.09 Air.in :default @14:19:45.15 Air.hold @14:19:45.71 Pulled 675uL lysate @15.9psi @14:19:45.91 Lysis1.seek 800uL,nil @14:19:47.31 Air.in :compressLysate @14:19:47.36 Air.seek :evac @14:19:52.82 Air.in :default @14:19:52.89 Air.hold @14:19:53.14 Air.in :glide @14:19:53.20 Air.seek :fullVent @14:19:55.23 Handoff.select :waste @14:19:55.75 Recovered 800uL lysate @15.5psi @14:19:55.78 delay 3 seconds @14:19:58.86 After 3 delay Recovered 800uL lysate @0.9psi @14:19:59.07 Handoff.select :close @14:19:59.58 Gate.power :heat,:OFF @14:19:59.60 Gate.power :cartridge,:OFF @14:19:59.67 PinPort.in :unlocking @14:19:59.73 PinPort.seek :clear @14:20:00.57 Carousel.in :default @14:20:00.69 Carousel.home.seek 55 @14:20:01.03 Plunge.seek :locked @14:20:02.42 Archive.in :unlocking @14:20:02.55 Archive.seek :clear @14:20:02.64 Air.in :unlocking @14:20:02.77 Air.seek :clear @14:20:03.39 Lysis1.in :unlocking @14:20:03.50 Lysis1.seek :clear @14:20:03.62 Lysis2.in :unlocking @14:20:03.68 Lysis2.seek :clear @14:20:07.56 Gate.power :analytic3,:OFF @14:20:07.58 Gate.power :motor,:OFF @14:20:07.59 Gate.power :analytic1,:OFF @14:20:07.61 Gate.power :analytic2,:OFF @14:20:07.62 Gate.power :core,:OFF @14:20:07.63 Gate.power :main,:OFF @14:20:07.65 Safely stopped and ready to power off @15:10:36.04 Session Begun @15:10:55.37 -> ESP.ready! @15:10:55.43 Gate.power :main,:ON,limit to 5000mA @15:10:55.46 Gate.power :analytic1,:ON,limit to 4000mA @15:10:55.48 Gate.power :analytic2,:ON,limit to 4000mA @15:10:55.52 Gate.power :analytic3,:ON,limit to 4000mA @15:10:55.63 Powering core bus @15:10:57.98 SPR Module 1, FW Rev. B, FPGA Rev. C @15:10:58.42 Plunge.configure :default @15:10:58.52 SamplePump.configure :default @15:10:58.63 Toroid.configure :default @15:10:58.74 Carousel.configure :default @15:10:58.85 Abs.configure :default @15:10:58.97 PhaseSeparator.configure :default @15:10:59.07 Archive.configure :default @15:10:59.17 Air.configure :default @15:10:59.25 Lysis1.configure :default @15:10:59.33 Lysis2.configure :default @15:10:59.43 PinPort.configure :default @15:10:59.50 All elf controllers running firmware version 4.94 @15:10:59.53 Gate.power :motor,:ON,limit to 5000mA @15:10:59.74 Plunge.home.jog 0 @15:11:00.63 PinPort.home.jog 0 @15:11:00.67 Lysis1.home.jog 0 @15:11:00.72 Archive.home.jog 0 @15:11:00.76 Toroid.home.jog 0 @15:11:00.82 Lysis2.home.jog 0 @15:11:00.85 Carousel.home.jog 0 @15:11:00.90 Air.home.jog 0 @15:11:01.70 Plunge.in :homing @15:11:02.04 Plunge.jog 1479 @15:11:02.31 Lysis2.in :homing @15:11:02.38 Lysis1.in :homing @15:11:02.43 Archive.in :homing @15:11:02.49 PinPort.in :homing @15:11:02.52 Air.in :homing @15:11:02.55 Toroid.in :homing @15:11:02.89 Lysis2.jog 1621 @15:11:02.94 Lysis1.jog 1603 @15:11:02.97 Archive.jog 1615 @15:11:03.03 PinPort.jog 1232 @15:11:03.08 Air.jog 1427 @15:11:03.13 Toroid.jog 1362 @15:11:03.58 Plunge.setRawPosition! 50 @15:11:03.68 Plunge.seek :home @15:11:04.00 Plunge.in :default @15:11:04.45 Lysis2.setRawPosition! 50 @15:11:04.55 Lysis2.seek :home @15:11:04.68 Lysis1.setRawPosition! 50 @15:11:04.72 Air.setRawPosition! 0 @15:11:04.75 Archive.setRawPosition! 50 @15:11:04.94 Lysis1.seek :home @15:11:04.99 Air.seek :home @15:11:05.09 Archive.seek :home @15:11:05.88 Lysis2.in :default @15:11:05.94 Lysis1.in :default @15:11:05.97 Archive.in :default @15:11:06.01 PinPort.setRawPosition! 3150 @15:11:06.09 Air.in :default @15:11:06.36 PinPort.seek :home @15:11:07.99 Toroid.setRawPosition! 0 @15:11:08.06 Toroid.seek :home @15:11:08.19 Toroid.in :default @15:11:10.76 PinPort.in :default @15:11:11.12 Toroid.seek :clear @15:11:11.32 PinPort.in :unlocking @15:11:11.40 PinPort.seek :clear @15:11:14.76 Air.seek :clear @15:11:14.89 Lysis1.seek :clear @15:11:15.65 Lysis2.seek :clear @15:11:16.48 Archive.seek :clear @15:11:17.28 Carousel.in :default @15:11:17.44 Carousel.home.seek 55 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @3.75psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @15:11:31.94 -> ESP.shutdown @15:11:32.13 Gate.power :analytic3,:OFF @15:11:32.16 Gate.power :motor,:OFF @15:11:32.19 Gate.power :analytic1,:OFF @15:11:32.23 Gate.power :analytic2,:OFF @15:11:32.27 Gate.power :core,:OFF @15:11:32.31 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @15:13:46.04 -> MainThread.exit # @15:13:46.15 ThreadError in preston -- killed thread @15:13:46.22 Session Ended @16:04:59.80EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @16:05:03.61 >BREAK< ESP3 Gateway v1.44 for core bus @16:05:03.64 >BREAK< ESP3 Gateway v1.44 for cartridge bus @16:05:07.41 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @16:05:08.12 Sea pressure (Abs) tare is 6% of full scale @16:05:11.84 Polling Can every 2:00 @16:05:11.89 Waiting for espclients... @16:05:13.10 Session Begun @16:05:14.84 -> ESP.ready! @16:05:14.90 Gate.power :sampler,:OFF,limit to 3500mA @16:05:14.93 Gate.power :skimmer,:OFF,limit to 3500mA @16:05:14.96 Gate.power :main,:ON,limit to 5000mA @16:05:15.00 Gate.power :analytic1,:ON,limit to 4000mA @16:05:15.19 Powering core bus @16:05:17.43 SPR Module 1, FW Rev. B, FPGA Rev. C @16:05:17.99 Plunge.configure :default @16:05:18.09 SamplePump.configure :default @16:05:18.19 Toroid.configure :default @16:05:18.32 Carousel.configure :default @16:05:18.43 Abs.configure :default @16:05:18.53 PhaseSeparator.configure :default @16:05:18.63 Archive.configure :default @16:05:18.74 Air.configure :default @16:05:18.82 Lysis1.configure :default @16:05:18.90 Lysis2.configure :default @16:05:19.00 PinPort.configure :default @16:05:19.09 All elf controllers running firmware version 4.94 @16:05:19.13 Gate.power :motor,:ON,limit to 5000mA @16:05:19.32 Plunge.home.jog 0 @16:05:20.12 PinPort.home.jog 0 @16:05:20.17 Lysis1.home.jog 0 @16:05:20.22 Archive.home.jog 0 @16:05:20.29 Toroid.home.jog 0 @16:05:20.34 Lysis2.home.jog 0 @16:05:20.37 Carousel.home.jog 0 @16:05:20.42 Air.home.jog 0 @16:05:21.19 Plunge.in :homing @16:05:21.58 Plunge.jog 429 @16:05:21.67 PinPort.in :homing @16:05:21.72 Lysis2.in :homing @16:05:21.77 Air.in :homing @16:05:21.82 Archive.in :homing @16:05:21.86 Toroid.in :homing @16:05:21.90 Lysis1.in :homing @16:05:22.22 PinPort.jog 1226 @16:05:22.32 Lysis2.jog 1615 @16:05:22.36 Air.jog 1428 @16:05:22.42 Archive.jog 1614 @16:05:22.47 Toroid.jog 1360 @16:05:22.51 Lysis1.jog 1597 @16:05:22.87 Plunge.setRawPosition! 50 @16:05:22.96 Plunge.seek :home @16:05:23.26 Plunge.in :default @16:05:23.85 Air.setRawPosition! 0 @16:05:23.93 Air.seek :home @16:05:24.08 Archive.setRawPosition! 50 @16:05:24.11 Lysis2.setRawPosition! 50 @16:05:24.26 Archive.seek :home @16:05:24.32 Lysis2.seek :home @16:05:24.54 Lysis1.setRawPosition! 50 @16:05:24.97 Air.in :default @16:05:25.02 PinPort.setRawPosition! 3150 @16:05:25.15 Lysis1.seek :home @16:05:25.24 PinPort.seek :home @16:05:25.51 Archive.in :default @16:05:25.55 Lysis2.in :default @16:05:25.74 Lysis1.in :default @16:05:27.26 Toroid.setRawPosition! 0 @16:05:27.34 Toroid.seek :home @16:05:27.45 Toroid.in :default @16:05:29.78 PinPort.in :default @16:05:30.14 Toroid.seek :clear @16:05:30.36 PinPort.in :unlocking @16:05:30.44 PinPort.seek :clear @16:05:33.78 Air.seek :clear @16:05:33.91 Lysis1.seek :clear @16:05:34.65 Lysis2.seek :clear @16:05:35.47 Archive.seek :clear @16:05:36.31 Carousel.in :default @16:05:36.46 Carousel.home.seek 53 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 53 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @0.17psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:05:50.37 -> Power.sampler :ON @16:05:50.41 Gate.power :sampler,:ON true @16:06:01.14 -> Power.sampler :OFF @16:06:01.16 Gate.power :sampler,:OFF false @16:08:57.75 -> Power.sampler :ON @16:08:57.78 Gate.power :sampler,:ON true @16:09:57.86 -> 2.times{Sampler.refill};BACsampler.prime 10,10 @16:09:57.89 Bypass.open @16:09:59.48 Intake.open @16:10:01.01 SamplePump.setPosition! 0ml @16:10:01.15 SamplePump.seek 30ml @16:10:41.12 Bypass.close @16:10:42.66 Exhaust.open @16:10:44.29 SamplePump.seek 55ml @16:11:17.71 delay 1 second @16:11:18.74 Intake.close @16:11:18.84 Exhaust.close @16:11:20.45 Bypass.open @16:11:22.03 Intake.open @16:11:23.50 SamplePump.setPosition! 0ml @16:11:23.64 SamplePump.seek 30ml @16:11:39.93 SamplePump.hold @16:11:40.20 delay 1 second @16:11:41.24 Intake.close @16:11:41.35 Bypass.close @16:11:43.08 UserAbort in nadia -- Sample Pump (SP) seek to 30ml Aborted! @16:12:47.89 -> BACsampler.prime 10,10 @16:12:48.00 Priming sample loop w/10ml, bypass w/10ml @16:12:48.02 SamplePump.setPosition! 0ml @16:12:48.11 Bypass.open @16:12:50.47 Intake.open @16:12:52.04 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:12:54.65 Vacuum tare is 54% of full scale at 25C (drifted 2%) @16:12:54.83 Abs.in :default @16:12:54.90 Abs.hold @16:12:55.00 PhaseSeparator.in :full @16:12:55.08 PhaseSeparator.hold @16:12:55.35 SamplePump.in :start @16:12:55.45 SamplePump.seek 10ml,1:10 @16:12:56.04 SamplePump.configure :prime @16:13:09.00 Abs.stop @16:13:09.07 PhaseSeparator.stop @16:13:09.14 SamplePump.setPosition! 0ml @16:13:09.22 Exhaust.open @16:13:11.43 Bypass.close @16:13:13.85 Abs.in :default @16:13:13.93 Abs.hold @16:13:14.03 PhaseSeparator.hold @16:13:14.41 SamplePump.in :start @16:13:14.51 SamplePump.seek 10ml,1:10 @16:13:15.10 SamplePump.configure :prime @16:13:28.08 Abs.stop @16:13:28.15 PhaseSeparator.stop BACsampler @16:13:39.70 -> Power.sampler :OFF @16:13:39.73 Gate.power :sampler,:OFF false @16:14:13.95 -> Cart.release Cart.reserve @16:14:14.01 Cartridge::Error in nadia -- Slot (0) is not in 1..60 @16:14:34.98 -> Cart.release Cart.reserved 4 @16:14:38.34 -> slots {[40,20] => { Type: :microSPR_phyto, State: :dry}, [50,45] => { Type: :archiveHiBiomass_bac, State: :dry}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @16:16:18.62 -> start {fire archive, lysis} # @16:16:19.58 Toroid.seek :clear @16:16:19.79 Air.seek :clear @16:16:19.91 Lysis1.seek :clear @16:16:20.03 Lysis2.seek :clear @16:16:20.14 Archive.seek :clear @16:16:20.27 Carousel.in :default @16:16:20.45 Carousel.home.seek 53 @16:16:20.75 Selecting Cartridge 50 @16:16:21.07 Carousel.seek 50 @16:16:22.07 Carousel.reconfigure :align @16:16:22.22 Lysis1.in :fast @16:16:22.33 Lysis1.seek :locked @16:16:24.98 Plunge.seek :locked @16:16:25.68 Air.in :fast @16:16:25.76 Air.seek :locked @16:16:26.85 Carousel.reconfigure :locked @16:16:27.03 Powering cartridge bus @16:16:29.70 Gate.setIR 1750,5000,0,6 @16:16:29.83 Gate.setIR 0,5000,0,6 @16:16:29.95 Gate.power :cartridge,:OFF @16:16:30.00 Mounted archive Cartridge @16:16:30.39 Preparing to filter archiveHiBiomass_bac cartridge in slot 50 @16:16:30.66 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @16:16:37.27 Intake Head Pressure tare is 1% of full scale at 25C (drifted 1%) @16:16:37.29 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @16:16:37.36 PhaseSeparator.in :filling @16:16:37.43 PhaseSeparator.hold @16:16:37.53 Gate.power :sampler,:ON @16:16:44.69 Head pressure reached 3.22psi in 8 seconds @16:16:44.87 Priming sample loop w/200ml, bypass w/10ml @16:16:44.89 SamplePump.setPosition! 0ml @16:16:44.98 Exhaust.close @16:16:46.52 Bypass.open @16:16:49.02 Abs.in :default @16:16:49.10 Abs.hold @16:16:49.20 PhaseSeparator.in :full @16:16:49.27 PhaseSeparator.hold @16:16:49.52 Waiting for sampler to finish priming... @16:16:49.54 delayUntil "16:17:37.55" @16:17:37.62 SamplePump.in :start @16:17:37.72 SamplePump.seek 10ml,1:10 @16:17:38.31 SamplePump.configure :prime @16:17:51.27 Abs.stop @16:17:51.35 PhaseSeparator.stop @16:17:51.41 SamplePump.setPosition! 0ml @16:17:51.50 Exhaust.open @16:17:53.77 Bypass.close @16:17:56.14 Abs.in :default @16:17:56.23 Abs.hold @16:17:56.38 PhaseSeparator.hold @16:17:56.73 SamplePump.in :start @16:17:57.34 SamplePump.seek 200ml,13:50 @16:17:57.45 SamplePump.configure :prime @16:22:19.93 Abs.stop @16:22:20.01 PhaseSeparator.stop @16:22:20.11 Bypass.open @16:22:21.65 Intake.close @16:22:23.27 Toroid.in :hold @16:22:23.36 Toroid.seek :crack @16:22:25.87 Toroid.seek :cartridge @16:22:28.03 Toroid.in :default @16:22:28.12 Toroid.seek :clear @16:22:32.12 Pressurizing cartridge to 26psi above 15.2psia @16:22:32.14 SamplePump.setPosition! 0ml @16:22:32.24 Intake.open @16:22:33.71 Bypass.close @16:22:35.24 Exhaust.close @16:22:36.97 Abs.in :default @16:22:37.05 Abs.hold @16:22:37.16 PhaseSeparator.hold @16:22:37.43 SamplePump.in :charge @16:22:37.52 SamplePump.seek 2.3ml,40 seconds @16:22:54.36 Abs.stop @16:22:54.44 PhaseSeparator.stop @16:22:54.53 Intake.close @16:22:56.11 Pressurized cartridge with 1.171ml to 26.2psi @16:22:56.13 Isolated @34.9psia (19.7psi) @16:22:56.19 Verifying cartridge holds at least 0.65..0.85 of 34.9psia @16:22:56.25 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:23:04.41 Cartridge pressure fell 19% to 28.1psia @16:23:04.58 Toroid.in :hold @16:23:04.66 Toroid.seek :bypass @16:23:08.32 Toroid.in :default @16:23:08.41 Toroid.seek :clear @16:23:12.12 Cartridge::Sampler::PossibleLeak in FILTERING -- Retained only 28.1psia @16:23:12.14 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @16:23:15.01 Gate.power :sampler,:OFF @16:23:33.71 -> SC Storage Carousel (SC) at 50 @16:23:42.90 -> slot 50=>:dry {50 => { Type: :archiveHiBiomass_bac, State: :dry}} @16:23:51.54 -> Cart.unlock @16:23:51.65 Carousel.in :default @16:23:51.80 Carousel.home.seek 50 @16:23:52.35 Air.in :unlocking @16:23:52.50 Air.seek :clear @16:23:53.60 Lysis1.in :unlocking @16:23:53.74 Lysis1.seek :clear @16:23:53.88 Plunge.in :unlocking @16:23:53.96 Plunge.seek :clear @16:24:37.70 -> Thread["detached in nadia"].abort @16:24:37.86 NoMethodError in nadia -- private method `abort' called for nil:NilClass @16:26:13.13 -> slots {[40,20] => { Type: :microSPR_phyto, State: :dry}, [50,45] => { Type: :archiveHiBiomass_bac, State: :dry}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @16:26:21.93 -> start {fire archive, lysis} @16:26:22.12 Selecting Cartridge 50 # @16:26:22.54 Carousel.seek 50 @16:26:22.83 Carousel.reconfigure :align @16:26:22.99 Lysis1.in :fast @16:26:23.07 Lysis1.seek :locked @16:26:25.70 Plunge.in :default @16:26:25.79 Plunge.seek :locked @16:26:25.98 Air.in :fast @16:26:26.06 Air.seek :locked @16:26:27.16 Carousel.reconfigure :locked @16:26:27.35 Powering cartridge bus @16:26:30.01 Gate.setIR 1750,5000,0,6 @16:26:30.14 Gate.setIR 0,5000,0,6 @16:26:30.26 Gate.power :cartridge,:OFF @16:26:30.34 Mounted archive Cartridge @16:26:30.72 Preparing to filter archiveHiBiomass_bac cartridge in slot 50 @16:26:30.75 Cmd::Error in detached from nadia -- Cannot process while in READY state @16:26:30.84 Waiting to Resume from Checkpoint "Selecting Cartridge 50" @16:26:31.06 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @16:26:37.68 Intake Head Pressure tare is 0% of full scale at 27C (drifted 1%) @16:26:37.70 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @16:26:37.77 PhaseSeparator.in :filling @16:26:37.83 PhaseSeparator.hold @16:26:37.93 Gate.power :sampler,:ON @16:26:45.15 Head pressure reached 3.14psi in 8 seconds @16:26:45.28 Priming sample loop w/20ml @16:26:45.30 SamplePump.setPosition! 0ml @16:26:45.37 Bypass.open @16:26:48.38 Exhaust.open @16:26:50.63 Bypass.close @16:26:50.66 Intake.open @16:26:53.10 Abs.in :default @16:26:53.18 Abs.hold @16:26:53.29 PhaseSeparator.in :full @16:26:53.38 PhaseSeparator.hold @16:26:53.70 Waiting for sampler to finish priming... @16:26:53.72 delayUntil "16:27:37.95" @16:27:38.01 SamplePump.in :start @16:27:38.10 SamplePump.seek 20ml,1:50 @16:27:38.70 SamplePump.configure :prime @16:28:04.77 Abs.stop @16:28:04.85 PhaseSeparator.stop @16:28:04.95 Bypass.open @16:28:06.49 Intake.close @16:28:08.10 Toroid.in :hold @16:28:08.19 Toroid.seek :crack @16:28:10.68 Toroid.seek :cartridge @16:28:12.83 Toroid.in :default @16:28:12.92 Toroid.seek :clear @16:28:16.92 Pressurizing cartridge to 26psi above 15.0psia @16:28:16.94 SamplePump.setPosition! 0ml @16:28:17.02 Intake.open @16:28:18.50 Bypass.close @16:28:20.03 Exhaust.close @16:28:21.70 Abs.in :default @16:28:21.78 Abs.hold @16:28:21.89 PhaseSeparator.hold @16:28:22.15 SamplePump.in :charge @16:28:22.24 SamplePump.seek 2.3ml,40 seconds @16:28:33.48 Abs.stop @16:28:33.56 PhaseSeparator.stop @16:28:33.65 Intake.close @16:28:35.24 Pressurized cartridge with 0.514ml to 27.1psi @16:28:35.26 Isolated @38.2psia (23.1psi) @16:28:35.29 Verifying cartridge holds at least 0.65..0.85 of 38.2psia @16:28:35.36 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:28:43.05 Cartridge pressure fell 12% to 33.5psia @16:28:43.18 Sampling 1000.0ml @16:28:43.20 SamplePump.setPosition! 0ml @16:28:43.29 Exhaust.open @16:28:44.84 Intake.open @16:28:46.54 Abs.in :default @16:28:46.62 Abs.hold @16:28:46.72 PhaseSeparator.hold @16:28:47.09 SamplePump.in :start @16:28:47.18 SamplePump.seek 1000ml,1:07:10 @16:28:47.78 SamplePump.configure :sample @16:28:50.18 Cartridge 50 filtering 2/1000ml at 0.76ml/s with 12.9psi vacuum @16:29:50.24 Cartridge 50 filtering 48/1000ml at 0.76ml/s with 16.6psi vacuum @16:30:50.23 Cartridge 50 filtering 93/1000ml at 0.76ml/s with 20.4psi vacuum @16:31:50.24 Cartridge 50 filtering 139/1000ml at 0.76ml/s with 22.7psi vacuum @16:32:50.24 Cartridge 50 filtering 184/1000ml at 0.75ml/s with 12.7psi vacuum 5.4m deep (22.6psia) @16:33:43.66 SamplePump.stop @16:33:43.75 Intake.close @16:33:43.87 Exhaust.close @16:33:44.12 Toroid.in :hold @16:33:44.22 Toroid.seek :bypass @16:33:47.89 Toroid.in :default @16:33:47.99 Toroid.seek :clear @16:33:50.24 Cartridge 50 filtering 223/1000ml at 0.66ml/s with 0.2psi vacuum @16:33:51.58 Abs.stop @16:33:51.66 PhaseSeparator.stop @16:33:52.09 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.69ml/s made only -35.7psi vacuum after 223.5ml @16:33:52.12 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 223.47ml" @16:33:54.97 Gate.power :sampler,:OFF @16:37:02.23 -> TV.bypass @16:37:02.39 Toroid.in :hold @16:37:02.47 Toroid.seek :bypass @16:37:06.09 Toroid.in :default @16:37:06.17 Toroid.seek :clear Toroid Valve (TV) clear @16:37:31.19 -> BACsampler.prime 50,nil @16:37:31.29 Priming sample loop w/50ml @16:37:31.31 SamplePump.setPosition! 0ml @16:37:31.39 Bypass.open @16:37:33.81 Exhaust.open @16:37:36.06 Bypass.close @16:37:36.09 Intake.open @16:37:39.17 Abs.in :default @16:37:39.26 Abs.hold @16:37:39.37 PhaseSeparator.hold @16:37:39.73 SamplePump.stop @16:37:39.88 Cartridge::Sampler::LostHeadPressure in nadia -- Head Pressure Fell to 0.02psi @16:37:39.91 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @16:37:39.97 PhaseSeparator.in :filling @16:37:40.05 PhaseSeparator.hold @16:37:40.18 Gate.power :sampler,:ON @16:37:47.42 Head pressure reached 3.24psi in 8 seconds @16:37:47.44 Abs.hold @16:37:47.56 PhaseSeparator.in :full @16:37:47.64 PhaseSeparator.hold @16:37:47.96 Waiting for sampler to finish priming... @16:37:47.98 delayUntil "16:38:40.20" @16:38:40.30 SamplePump.in :start @16:38:40.42 SamplePump.seek 50ml,3:50 @16:38:40.97 SamplePump.configure :prime @16:38:45.48 SamplePump.stop @16:38:45.57 Intake.close @16:38:45.67 Exhaust.close @16:38:47.31 Abs.stop @16:38:47.40 PhaseSeparator.stop @16:38:47.49 Cartridge::Sampler::Leaky in nadia -- Serious Leak or Stall -- 0.76ml/s made only -36.1psi vacuum after 3.5ml @16:39:03.45 -> 2.times{Sampler.refill} @16:39:03.48 Bypass.open @16:39:05.03 Intake.open @16:39:06.52 SamplePump.setPosition! 0ml @16:39:06.66 SamplePump.in :default @16:39:06.75 SamplePump.seek 30ml @16:39:46.69 Bypass.close @16:39:48.24 Exhaust.open @16:39:49.87 SamplePump.seek 55ml @16:40:23.27 delay 1 second @16:40:24.31 Intake.close @16:40:24.43 Exhaust.close @16:40:26.09 Bypass.open @16:40:27.64 Intake.open @16:40:29.13 SamplePump.setPosition! 0ml @16:40:29.27 SamplePump.seek 30ml @16:40:38.47 SamplePump.hold @16:40:38.70 delay 1 second @16:40:39.73 Intake.close @16:40:39.79 Bypass.close @16:40:41.55 UserAbort in nadia -- Sample Pump (SP) seek to 30ml Aborted! @16:40:47.62 -> BACsampler.prime 10,10 @16:40:47.86 Priming sample loop w/10ml, bypass w/10ml @16:40:47.90 SamplePump.setPosition! 0ml @16:40:48.05 Bypass.open @16:40:50.49 Intake.open @16:40:52.81 Abs.in :default @16:40:52.89 Abs.hold @16:40:52.98 PhaseSeparator.hold @16:40:53.86 SamplePump.in :start @16:40:53.96 SamplePump.seek 10ml,1:10 @16:40:54.56 SamplePump.configure :prime @16:41:07.51 Abs.stop @16:41:07.59 PhaseSeparator.stop @16:41:07.66 SamplePump.setPosition! 0ml @16:41:07.73 Exhaust.open @16:41:09.98 Bypass.close @16:41:12.39 Abs.in :default @16:41:12.46 Abs.hold @16:41:12.57 PhaseSeparator.hold @16:41:12.91 SamplePump.in :start @16:41:13.00 SamplePump.seek 10ml,1:10 @16:41:13.60 SamplePump.configure :prime @16:41:26.55 Abs.stop @16:41:26.61 PhaseSeparator.stop BACsampler @16:45:33.54 -> Cart.unlock @16:45:33.65 Carousel.in :default @16:45:33.78 Carousel.home.seek 50 @16:45:34.22 Air.in :unlocking @16:45:34.41 Air.seek :clear @16:45:35.50 Lysis1.in :unlocking @16:45:35.66 Lysis1.seek :clear @16:45:35.78 Plunge.in :unlocking @16:45:35.86 Plunge.seek :clear @16:46:34.82 -> slots {[40,20] => { Type: :microSPR_phyto, State: :dry}, 45 => { Type: :archiveHiBiomass_bac, State: :dry}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @16:46:53.54 -> slot 51=>[:dry,:archiveHiBiomass_bac] {51 => { Type: :archiveHiBiomass_bac, State: :dry}} @16:48:39.01 Session Begun @16:49:50.65 -> start {fire archive, lysis} @16:49:50.87 Selecting Cartridge 51 # @16:49:51.26 Carousel.seek 51 @16:49:51.88 Carousel.reconfigure :align @16:49:52.04 Lysis1.in :fast @16:49:52.14 Lysis1.seek :locked @16:49:54.81 Plunge.in :default @16:49:54.90 Plunge.seek :locked @16:49:55.10 Air.in :fast @16:49:55.19 Air.seek :locked @16:49:56.32 Carousel.reconfigure :locked @16:49:56.51 Powering cartridge bus @16:49:59.18 Gate.setIR 1750,5000,0,6 @16:49:59.32 Gate.setIR 0,5000,0,6 @16:49:59.45 Gate.power :cartridge,:OFF @16:49:59.52 Mounted archive Cartridge @16:50:00.52 Cmd::Error in detached from nadia#2 -- Cannot process while in READY state @16:50:00.60 Preparing to filter archiveHiBiomass_bac cartridge in slot 51 @16:50:00.64 Waiting to Resume from Checkpoint "Selecting Cartridge 50" @16:50:00.93 Gate.power :sampler,:OFF @16:50:01.33 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @16:50:07.95 Intake Head Pressure tare is 1% of full scale at 27C (drifted 1%) @16:50:07.97 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @16:50:08.07 PhaseSeparator.in :filling @16:50:08.16 PhaseSeparator.hold @16:50:08.29 Gate.power :sampler,:ON @16:50:13.75 Head pressure reached 3.07psi in 6 seconds @16:50:13.89 Priming sample loop w/20ml @16:50:13.91 SamplePump.setPosition! 0ml @16:50:14.03 Exhaust.close @16:50:15.60 Bypass.open @16:50:18.04 Exhaust.open @16:50:20.24 Bypass.close @16:50:22.72 Abs.in :default @16:50:22.82 Abs.hold @16:50:22.93 PhaseSeparator.in :full @16:50:23.03 PhaseSeparator.hold @16:50:23.40 Waiting for sampler to finish priming... @16:50:23.42 delayUntil "16:51:08.33" @16:51:08.39 SamplePump.in :start @16:51:08.51 SamplePump.seek 20ml,1:50 @16:51:09.09 SamplePump.configure :prime @16:51:35.19 Abs.stop @16:51:35.34 PhaseSeparator.stop @16:51:35.46 Bypass.open @16:51:37.09 Intake.close @16:51:38.75 Toroid.in :hold @16:51:38.85 Toroid.seek :crack @16:51:41.37 Toroid.seek :cartridge @16:51:43.54 Toroid.in :default @16:51:43.65 Toroid.seek :clear @16:51:47.67 Pressurizing cartridge to 26psi above 14.9psia @16:51:47.70 SamplePump.setPosition! 0ml @16:51:47.82 Intake.open @16:51:49.32 Bypass.close @16:51:50.91 Exhaust.close @16:51:53.16 Abs.in :default @16:51:53.30 Abs.hold @16:51:53.42 PhaseSeparator.hold @16:51:53.73 SamplePump.in :charge @16:51:53.84 SamplePump.seek 2.3ml,40 seconds @16:52:03.42 Abs.stop @16:52:03.52 PhaseSeparator.stop @16:52:03.62 Intake.close @16:52:05.23 Pressurized cartridge with 0.373ml to 26.2psi @16:52:05.25 Isolated @38.7psia (23.9psi) @16:52:05.32 Verifying cartridge holds at least 0.65..0.85 of 38.7psia @16:52:05.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:52:10.02 Cartridge pressure fell 6% to 36.4psia @16:52:10.16 Sampling 1000.0ml @16:52:10.18 SamplePump.setPosition! 0ml @16:52:10.35 Exhaust.open @16:52:11.93 Intake.open @16:52:13.67 Abs.in :default @16:52:13.77 Abs.hold @16:52:13.89 PhaseSeparator.hold @16:52:14.28 SamplePump.in :start @16:52:14.42 SamplePump.seek 1000ml,1:07:10 @16:52:14.96 SamplePump.configure :sample @16:52:17.37 Cartridge 51 filtering 2/1000ml at 0.76ml/s with 17.9psi vacuum 3.7m deep (20.2psia) @16:52:27.38 -> upsync @16:53:17.43 Cartridge 51 filtering 47/1000ml at 0.75ml/s with 18.5psi vacuum @16:54:17.42 Cartridge 51 filtering 91/1000ml at 0.74ml/s with 14.8psi vacuum @16:55:17.44 Cartridge 51 filtering 135/1000ml at 0.73ml/s with 20.8psi vacuum @16:56:17.43 Cartridge 51 filtering 178/1000ml at 0.72ml/s with 21.1psi vacuum @16:57:17.45 Cartridge 51 filtering 220/1000ml at 0.70ml/s with 18.0psi vacuum @16:58:17.42 Cartridge 51 filtering 262/1000ml at 0.69ml/s with 14.3psi vacuum @16:59:17.42 Cartridge 51 filtering 303/1000ml at 0.69ml/s with 20.8psi vacuum @17:00:17.42 Cartridge 51 filtering 344/1000ml at 0.68ml/s with 25.0psi vacuum @17:01:17.98 Cartridge 51 filtering 384/1000ml at 0.67ml/s with 20.3psi vacuum 4.0m deep (20.6psia) @17:02:17.43 Cartridge 51 filtering 423/1000ml at 0.65ml/s with 22.5psi vacuum @17:03:17.43 Cartridge 51 filtering 461/1000ml at 0.64ml/s with 32.8psi vacuum @17:03:28.87 Session Begun @17:03:39.53 -> can Can@17:03:39, 27.4C, 32% humidity, 14.3psia, 14.736V, 1.847A, 1.407Ah, 27.22W @17:03:52.64 -> can! @17:03:52.68 Polling Can every 2:00 Can@17:03:53, 27.4C, 32% humidity, 14.3psia, 14.744V, 1.846A, 1.414Ah, 27.22W @17:04:17.42 Cartridge 51 filtering 499/1000ml at 0.63ml/s with 18.0psi vacuum 3.8m deep (20.3psia) @17:05:14.01 -> H.psi @17:05:14.08 NameError in Benjamin#2 -- uninitialized constant H @17:05:17.42 Cartridge 51 filtering 536/1000ml at 0.62ml/s with 29.4psi vacuum @17:05:22.98 -> Head.psi 2.89543808134758 @17:06:06.21 -> Head.psi 2.98133859127684 @17:06:17.42 Cartridge 51 filtering 573/1000ml at 0.61ml/s with 24.8psi vacuum @17:06:46.09 -> Head.counts 17902 @17:07:17.42 Cartridge 51 filtering 609/1000ml at 0.60ml/s with 29.9psi vacuum @17:08:17.45 Cartridge 51 filtering 644/1000ml at 0.59ml/s with 29.2psi vacuum @17:09:17.45 Cartridge 51 filtering 678/1000ml at 0.57ml/s with 13.2psi vacuum 9.4m deep (28.6psia) @17:09:45.17 SamplePump.stop @17:09:45.31 Intake.close @17:09:45.44 Exhaust.close @17:09:45.78 Toroid.in :hold @17:09:45.91 Toroid.seek :bypass @17:09:49.56 Toroid.in :default @17:09:49.70 Toroid.seek :clear @17:09:53.33 Abs.stop @17:09:53.46 PhaseSeparator.stop @17:09:53.93 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.45ml/s made only -62.3psi vacuum after 693.9ml @17:09:53.95 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 693.86ml" @17:09:56.80 Gate.power :sampler,:OFF @17:10:32.74 -> Sampler.psi 0.488793692184004 @17:11:33.63 -> Head.psi 0.00286629011570937 @17:11:43.69 -> Head.count 1 @17:12:13.84 -> Head.counts 376 @17:12:21.48 -> Head.psi 0.00110241927527283 @17:12:29.46 -> Head.counts 374 @17:12:51.06 -> Power.sampler :ON @17:12:51.09 Gate.power :sampler,:ON true @17:13:59.86 -> Head.counts 7099 @17:14:16.55 -> Head.psi 1.17883897943475 @17:14:35.83 -> Head.counts 24931 @17:14:45.50 -> Head.psi 4.33916636424488 @17:15:15.87 -> Head.counts 17555 @17:15:41.85 -> Head.counts 8877 @17:16:45.21 -> Power.sampler :OFF @17:16:45.25 Gate.power :sampler,:OFF false @17:17:13.06 -> Power.sampler :ON @17:17:13.08 Gate.power :sampler,:ON true @17:18:31.86 -> Sampler.refill @17:18:31.89 Bypass.open @17:18:33.53 Intake.open @17:18:35.07 SamplePump.setPosition! 0ml @17:18:35.25 SamplePump.in :default @17:18:35.36 SamplePump.seek 30ml @17:19:01.70 SamplePump.hold @17:19:02.58 delay 1 second @17:19:03.61 Intake.close @17:19:03.69 Bypass.close @17:19:05.50 UserAbort in nadia -- Sample Pump (SP) seek to 30ml Aborted! @17:19:18.17 -> BACsampler.prime 50,nil @17:19:18.37 Priming sample loop w/50ml @17:19:18.39 SamplePump.setPosition! 0ml @17:19:18.54 Bypass.open @17:19:21.01 Exhaust.open @17:19:23.27 Bypass.close @17:19:23.34 Intake.open @17:19:25.80 Abs.in :default @17:19:25.92 Abs.hold @17:19:26.05 PhaseSeparator.hold @17:19:26.46 SamplePump.in :start @17:19:26.60 SamplePump.seek 50ml,3:50 @17:19:27.15 SamplePump.configure :prime @17:20:03.47 SamplePump.stop @17:20:03.60 Intake.close @17:20:03.66 Exhaust.close @17:20:05.40 Abs.stop @17:20:05.51 PhaseSeparator.stop @17:20:05.62 Cartridge::Sampler::Leaky in nadia -- Serious Leak or Stall -- 0.76ml/s made only -107.6psi vacuum after 27.8ml @17:20:31.13 -> Sampler.psi 1.0573904361531 @17:20:43.93 -> Leak.psia 15.1039079632292 @17:21:12.67 Session Ended @17:21:12.74 Session Ended @17:21:12.82 Errno::EPIPE in Benjamin#2's_reader -- Broken pipe @17:22:09.50 Session Begun @17:22:30.24 -> loop{puts Sampler.psi;Delay.sleep 0.5} @17:22:44.98 -> Sampler.tare @17:22:45.04 Cartridge::Sampler::Error in nadia -- Cannot tare with Intake closed @17:22:51.78 -> Sampler.tare! @17:22:51.80 Bypass.open @17:22:53.92 Intake.open @17:22:54.10 UserAbort in nadia -- deferring User Abort!! @17:22:54.17 until Intake finishes opening @17:22:55.43 Bypass.close @17:22:55.47 Intake.close @17:22:57.13 UserAbort in nadia -- User Abort!! @17:23:04.49 -> Power.sampler :OFF @17:23:04.52 Gate.power :sampler,:OFF false @17:23:12.69 -> Sampler.tare! @17:23:12.73 Bypass.open @17:23:14.84 Intake.open @17:23:16.50 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:23:19.12 Vacuum tare is 54% of full scale at 27C (drifted 2%) @17:23:19.25 Bypass.close @17:23:19.33 Intake.close 0.004987691(PSI/count)-175.8 @17:23:35.00 -> Power.sampler :ON @17:23:35.12 Gate.power :sampler,:ON true @17:23:48.97 -> 2.times{Sampler.refill} @17:23:49.00 Bypass.open @17:23:50.62 Intake.open @17:23:52.13 SamplePump.setPosition! 0ml @17:23:52.32 SamplePump.in :default @17:23:52.43 SamplePump.seek 30ml @17:24:32.39 Bypass.close @17:24:34.02 Exhaust.open @17:24:35.69 SamplePump.seek 55ml @17:25:09.11 delay 1 second @17:25:10.14 Intake.close @17:25:10.19 Exhaust.close @17:25:12.04 Bypass.open @17:25:13.61 Intake.open @17:25:15.15 SamplePump.setPosition! 0ml @17:25:15.31 SamplePump.seek 30ml @17:25:19.86 SamplePump.hold @17:25:20.17 delay 1 second @17:25:21.21 Intake.close @17:25:21.26 Bypass.close @17:25:22.96 UserAbort in nadia -- Sample Pump (SP) seek to 30ml Aborted! @17:25:35.46 -> BACsampler.prime 50,nil @17:25:35.72 Priming sample loop w/50ml @17:25:35.74 SamplePump.setPosition! 0ml @17:25:35.86 Bypass.open @17:25:38.33 Exhaust.open @17:25:40.62 Bypass.close @17:25:40.71 Intake.open @17:25:43.06 Abs.in :default @17:25:43.17 Abs.hold @17:25:43.39 PhaseSeparator.hold @17:25:43.77 SamplePump.in :start @17:25:43.88 SamplePump.seek 50ml,3:50 @17:25:44.46 SamplePump.configure :prime @17:26:49.92 Abs.stop @17:26:50.01 PhaseSeparator.stop BACsampler @17:29:14.24 Session Begun @17:29:16.50 -> BACsampler.prime 50,nil @17:29:16.80 Priming sample loop w/50ml @17:29:16.84 SamplePump.setPosition! 0ml @17:29:16.97 Exhaust.close @17:29:18.59 Bypass.open @17:29:21.06 Exhaust.open @17:29:23.32 Bypass.close @17:29:25.73 Abs.in :default @17:29:25.86 Abs.hold @17:29:26.01 PhaseSeparator.hold @17:29:26.57 SamplePump.in :start @17:29:26.69 SamplePump.seek 50ml,3:50 @17:29:27.27 SamplePump.configure :prime @17:30:32.77 Abs.stop @17:30:32.87 PhaseSeparator.stop BACsampler @17:30:46.27 UserAbort in na -- User Abort!! @17:36:08.10 -> Power.sampler :OFF @17:36:08.17 Gate.power :sampler,:OFF false @17:36:54.75 -> ESP.shutdown @17:36:54.90 Intake.close @17:36:55.04 Exhaust.close @17:36:56.76 Gate.power :motor,:OFF @17:36:56.78 Gate.power :analytic1,:OFF @17:36:56.85 Gate.power :core,:OFF @17:36:56.92 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @17:37:44.46 Session Ended @17:43:58.57 -> slot 51 {51 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 693.9}} @17:44:06.58 -> slot 51=>:dry {51 => { Type: :archiveHiBiomass_bac, State: :dry, filtered: 693.9}} @17:44:30.74 -> start {fire archive,lysis} @17:44:30.96 Gate.power :sampler,:OFF,limit to 3500mA # @17:44:31.02 Gate.power :skimmer,:OFF,limit to 3500mA @17:44:31.12 Gate.power :main,:ON,limit to 5000mA @17:44:31.16 Gate.power :analytic1,:ON,limit to 4000mA @17:44:31.28 Powering core bus @17:44:33.62 SPR Module 1, FW Rev. B, FPGA Rev. C @17:44:34.06 Plunge.configure :default @17:44:34.19 SamplePump.configure :default @17:44:34.34 Toroid.configure :default @17:44:34.46 Carousel.configure :locked @17:44:34.58 Abs.configure :default @17:44:34.69 PhaseSeparator.configure :full @17:44:34.80 Archive.configure :default @17:44:34.92 Air.configure :default @17:44:35.01 Lysis1.configure :default @17:44:35.10 Lysis2.configure :default @17:44:35.22 PinPort.configure :default @17:44:35.32 All elf controllers running firmware version 4.94 @17:44:35.35 Gate.power :motor,:ON,limit to 5000mA @17:44:36.07 Plunge.home.jog 0 @17:44:36.23 Toroid.home.jog 0 @17:44:36.58 Lysis2.home.jog 0 @17:44:36.64 Lysis1.home.jog 0 @17:44:36.69 Archive.home.jog 0 @17:44:36.74 Carousel.in :default @17:44:36.80 PinPort.home.jog 0 @17:44:36.86 Air.home.jog 0 @17:44:37.20 Carousel.home.jog 0 @17:44:38.06 Lysis2.in :homing @17:44:38.14 Lysis1.in :homing @17:44:38.20 Archive.in :homing @17:44:38.26 Plunge.in :homing @17:44:38.36 PinPort.in :homing @17:44:38.41 Air.in :homing @17:44:38.46 Toroid.in :homing @17:44:39.39 Lysis2.jog 1618 @17:44:39.63 Lysis1.jog 2267 @17:44:39.72 Air.jog 1243 @17:44:39.79 Toroid.jog 1362 @17:44:39.86 Archive.jog 1615 @17:44:39.93 PinPort.jog 1223 @17:44:39.99 Plunge.jog 1463 @17:44:41.31 Air.setRawPosition! 0 @17:44:41.41 Air.seek :home @17:44:41.56 Lysis2.setRawPosition! 50 @17:44:41.58 Air.in :default @17:44:41.76 Lysis2.seek :home @17:44:41.94 Lysis1.setRawPosition! 50 @17:44:41.97 Archive.setRawPosition! 50 @17:44:42.02 Plunge.setRawPosition! 50 @17:44:42.22 Lysis1.seek :home @17:44:42.25 Archive.seek :home @17:44:42.51 Plunge.seek :home @17:44:42.75 Lysis2.in :default @17:44:42.79 Lysis1.in :default @17:44:42.85 PinPort.setRawPosition! 3150 @17:44:43.10 PinPort.seek :home @17:44:43.28 Plunge.in :default @17:44:43.31 Archive.in :default @17:44:44.84 Toroid.setRawPosition! 0 @17:44:44.94 Toroid.seek :home @17:44:45.07 Toroid.in :default @17:44:47.53 PinPort.in :default @17:44:47.92 Toroid.seek :clear @17:44:48.12 PinPort.in :unlocking @17:44:48.23 PinPort.seek :clear @17:44:51.59 Air.seek :clear @17:44:51.75 Lysis1.seek :clear @17:44:52.50 Lysis2.seek :clear @17:44:53.35 Archive.seek :clear @17:44:54.19 Carousel.in :default @17:44:54.38 Carousel.home.seek 51 @17:44:54.69 Selecting Cartridge 51 @17:44:55.05 Carousel.seek 51 @17:44:55.37 Carousel.reconfigure :align @17:44:55.53 Lysis1.in :fast @17:44:55.62 Lysis1.seek :locked @17:44:58.34 Plunge.seek :locked @17:44:58.57 Air.in :fast @17:44:58.65 Air.seek :locked @17:45:00.20 Carousel.reconfigure :locked @17:45:00.46 Powering cartridge bus @17:45:03.12 Gate.setIR 1750,5000,0,6 @17:45:03.25 Gate.setIR 0,5000,0,6 @17:45:03.40 Gate.power :cartridge,:OFF @17:45:03.47 Mounted archive Cartridge @17:45:03.84 Cmd::Error in detached from Benjamin -- Cannot process while in READY state @17:45:03.90 Preparing to filter archiveHiBiomass_bac cartridge in slot 51 @17:45:03.97 Waiting to Resume from Checkpoint "Selecting Cartridge 51" @17:45:04.26 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @17:45:10.90 Intake Head Pressure tare is 1% of full scale at 27C (drifted 1%) @17:45:10.92 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @17:45:11.02 PhaseSeparator.in :filling @17:45:11.10 PhaseSeparator.hold @17:45:11.22 Gate.power :sampler,:ON @17:45:18.47 Head pressure reached 3.22psi in 8 seconds @17:45:18.61 Priming sample loop w/200ml, bypass w/10ml @17:45:18.63 SamplePump.setPosition! 0ml @17:45:18.74 Bypass.open @17:45:21.24 Intake.open @17:45:23.63 Abs.in :default @17:45:23.73 Abs.hold @17:45:23.84 PhaseSeparator.in :full @17:45:23.92 PhaseSeparator.hold @17:45:24.19 Waiting for sampler to finish priming... @17:45:24.21 delayUntil "17:46:11.24" @17:45:44.84 Session Begun @17:45:58.56 -> exit @17:45:58.59 Session Ended @17:45:59.82 -> Head.psi 3.39439304533606 @17:46:11.31 SamplePump.in :start @17:46:11.43 SamplePump.seek 10ml,1:10 @17:46:12.00 SamplePump.configure :prime @17:46:25.01 Abs.stop @17:46:25.11 PhaseSeparator.stop @17:46:25.22 SamplePump.setPosition! 0ml @17:46:25.31 Exhaust.open @17:46:27.57 Bypass.close @17:46:30.64 Abs.in :default @17:46:30.78 Abs.hold @17:46:30.89 PhaseSeparator.hold @17:46:31.27 SamplePump.in :start @17:46:31.38 SamplePump.seek 200ml,13:50 @17:46:31.96 SamplePump.configure :prime @17:50:54.28 Abs.stop @17:50:54.40 PhaseSeparator.stop @17:50:54.51 Bypass.open @17:50:56.11 Intake.close @17:50:57.74 Toroid.in :hold @17:50:57.84 Toroid.seek :crack @17:51:00.34 Toroid.seek :cartridge @17:51:02.52 Toroid.in :default @17:51:02.63 Toroid.seek :clear @17:51:06.64 Pressurizing cartridge to 26psi above 14.6psia @17:51:06.67 SamplePump.setPosition! 0ml @17:51:06.79 Intake.open @17:51:08.34 Bypass.close @17:51:09.88 Exhaust.close @17:51:11.61 Abs.in :default @17:51:11.71 Abs.hold @17:51:11.83 PhaseSeparator.hold @17:51:12.12 SamplePump.in :charge @17:51:12.23 SamplePump.seek 2.3ml,40 seconds @17:51:17.48 Abs.stop @17:51:17.58 PhaseSeparator.stop @17:51:17.67 Intake.close @17:51:19.29 Pressurized cartridge with 0.106ml to 26.2psi @17:51:19.31 Isolated @36.8psia (22.2psi) @17:51:19.38 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @17:51:19.44 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:51:25.07 Cartridge pressure fell 11% to 32.7psia @17:51:25.87 Sampling 306.1ml @17:51:25.90 SamplePump.setPosition! 0ml @17:51:26.01 Exhaust.open @17:51:27.57 Intake.open @17:51:29.30 Abs.in :default @17:51:29.40 Abs.hold @17:51:29.54 PhaseSeparator.hold @17:51:29.93 SamplePump.in :start @17:51:30.06 SamplePump.seek 306.1ml,20:55 @17:51:30.62 SamplePump.configure :sample @17:51:33.03 Cartridge 51 filtering 2/306ml at 0.58ml/s with 25.1psi vacuum @17:52:33.09 Cartridge 51 filtering 39/306ml at 0.62ml/s with 20.7psi vacuum @17:53:33.09 Cartridge 51 filtering 75/306ml at 0.59ml/s with 14.6psi vacuum 8.3m deep (27.0psia) @17:54:33.08 Cartridge 51 filtering 109/306ml at 0.57ml/s with 26.8psi vacuum @17:55:33.09 Cartridge 51 filtering 141/306ml at 0.53ml/s with 16.6psi vacuum 7.5m deep (25.8psia) @17:56:33.09 Cartridge 51 filtering 171/306ml at 0.50ml/s with 26.4psi vacuum @17:57:33.09 Cartridge 51 filtering 199/306ml at 0.48ml/s with 24.6psi vacuum @17:58:33.09 Cartridge 51 filtering 226/306ml at 0.45ml/s with 27.1psi vacuum @17:59:33.09 Cartridge 51 filtering 252/306ml at 0.43ml/s with 22.9psi vacuum @18:00:33.08 Cartridge 51 filtering 277/306ml at 0.41ml/s with 27.9psi vacuum @18:01:33.09 Cartridge 51 filtering 300/306ml at 0.39ml/s with 22.2psi vacuum @18:01:48.86 Abs.stop @18:01:48.96 PhaseSeparator.stop @18:01:49.07 Intake.close @18:01:49.17 Exhaust.close @18:01:49.46 Toroid.in :hold @18:01:49.55 Toroid.seek :bypass @18:01:53.18 Toroid.in :default @18:01:53.29 Toroid.seek :clear @18:01:56.90 Sampled 306.1ml @18:01:57.55 Plunge.seek :pTop @18:01:58.50 Sample loop closed. Ready to Deliver Reagents @18:01:58.56 Air.in :default @18:01:58.67 Air.seek :sealed @18:02:00.07 Gate.power :sampler,:OFF @18:02:01.45 Air.in :slow @18:02:01.56 Air.seek :arEvac @18:03:16.15 delay 1:00 @18:04:16.79 Air.in :default @18:04:16.95 Air.seek :clear @18:04:35.69 RNAlater soak for 10:00 @18:14:35.73 Air.seek :tVent @18:14:38.29 delay 1 second @18:14:39.33 Air.in :halfSpeed @18:14:39.44 Air.seek :arEmpty @18:15:12.19 delay 10 seconds @18:15:22.23 delay 1:00 @18:16:22.28 Plunge.seek :pTop @18:16:22.45 Sample preserved and evacuated @18:16:22.62 Selecting Cartridge 40 @18:16:22.92 Carousel.in :default @18:16:23.08 Carousel.home.seek 51 @18:16:23.56 Air.in :unlocking @18:16:23.74 Air.seek :clear @18:16:28.57 Lysis1.in :unlocking @18:16:28.73 Lysis1.seek :clear @18:16:28.88 Plunge.in :unlocking @18:16:28.99 Plunge.seek :clear @18:16:30.47 Carousel.seek 40 @18:16:33.12 Carousel.reconfigure :align @18:16:33.29 Lysis1.in :fast @18:16:33.40 Lysis1.seek :locked @18:16:36.08 Plunge.in :default @18:16:36.17 Plunge.seek :locked @18:16:36.42 Air.in :fast @18:16:36.52 Air.seek :locked @18:16:37.63 Carousel.reconfigure :locked @18:16:37.83 Powering cartridge bus @18:16:40.50 Gate.setIR 1750,5000,0,6 @18:16:40.65 Gate.setIR 0,5000,0,6 @18:16:40.75 PuckHeater.configure :default @18:16:40.86 Gate.power :heat,:ON,limit to 5000mA @18:16:41.14 Mounted lysis Cartridge board#J59986 @18:16:41.22 Using default cartridge heat loss curve for HeatingFunctionInCarousel @18:16:41.58 Preparing to filter microSPR_phyto cartridge in slot 40 @18:16:41.87 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @18:16:48.51 Intake Head Pressure tare is 1% of full scale at 28C (drifted 1%) @18:16:48.61 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @18:16:48.72 PhaseSeparator.in :filling @18:16:48.79 PhaseSeparator.hold @18:16:48.91 Gate.power :sampler,:ON @18:16:56.01 Head pressure reached 3.38psi in 8 seconds @18:16:56.07 Air.in :default @18:16:56.24 Priming sample loop w/200ml, bypass w/10ml @18:16:56.33 Air.seek :sealed @18:16:56.39 SamplePump.setPosition! 0ml @18:16:56.56 Bypass.open @18:16:58.92 Intake.open @18:17:00.54 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:17:03.16 Vacuum tare is 54% of full scale at 28C (drifted 2%) @18:17:03.37 Abs.in :default @18:17:03.47 Abs.hold @18:17:03.59 PhaseSeparator.in :full @18:17:03.69 PhaseSeparator.hold @18:17:03.97 Waiting for sampler to finish priming... @18:17:03.99 delayUntil "18:17:48.94" @18:17:49.00 SamplePump.in :start @18:17:49.13 SamplePump.seek 10ml,1:10 @18:17:49.70 SamplePump.configure :prime @18:18:02.70 Abs.stop @18:18:02.80 PhaseSeparator.stop @18:18:02.89 SamplePump.setPosition! 0ml @18:18:02.97 Exhaust.open @18:18:05.28 Bypass.close @18:18:07.73 Abs.in :default @18:18:07.83 Abs.hold @18:18:07.95 PhaseSeparator.hold @18:18:08.36 SamplePump.in :start @18:18:08.48 SamplePump.seek 200ml,13:50 @18:18:09.05 SamplePump.configure :prime @18:22:31.99 Abs.stop @18:22:32.13 PhaseSeparator.stop @18:22:32.24 Bypass.open @18:22:33.83 Intake.close @18:22:35.47 Toroid.in :hold @18:22:35.57 Toroid.seek :crack @18:22:38.09 Toroid.seek :cartridge @18:22:40.27 Toroid.in :default @18:22:40.41 Toroid.seek :clear @18:22:44.47 Pressurizing cartridge to 14psi above 14.6psia @18:22:44.50 SamplePump.setPosition! 0ml @18:22:44.61 Intake.open @18:22:46.12 Bypass.close @18:22:47.66 Exhaust.close @18:22:49.43 Abs.in :default @18:22:49.54 Abs.hold @18:22:49.66 PhaseSeparator.hold @18:22:49.95 SamplePump.in :charge @18:22:50.05 SamplePump.seek 2.3ml,38 seconds @18:23:02.62 Abs.stop @18:23:02.73 PhaseSeparator.stop @18:23:02.84 Intake.close @18:23:04.47 Pressurized cartridge with 0.650ml to 14.1psi @18:23:04.49 Isolated @27.8psia (13.2psi) @18:23:04.55 Verifying cartridge holds at least 0.65..0.85 of 27.8psia @18:23:04.62 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:23:11.76 Cartridge pressure fell 13% to 24.3psia @18:23:11.91 Sampling 100.0ml @18:23:11.94 SamplePump.setPosition! 0ml @18:23:12.06 Exhaust.open @18:23:13.63 Intake.open @18:23:15.39 Abs.in :default @18:23:15.49 Abs.hold @18:23:16.20 PhaseSeparator.hold @18:23:16.74 SamplePump.in :start @18:23:16.86 SamplePump.seek 100ml,6:04 @18:23:17.43 SamplePump.configure :sample @18:23:19.84 Cartridge 40 filtering 2/100ml at 0.59ml/s with 13.2psi vacuum @18:24:19.89 Cartridge 40 filtering 39/100ml at 0.63ml/s with 12.6psi vacuum @18:25:19.89 Cartridge 40 filtering 73/100ml at 0.56ml/s with 17.9psi vacuum @18:26:11.64 Abs.stop @18:26:11.74 PhaseSeparator.stop @18:26:11.87 Intake.close @18:26:11.91 Exhaust.close @18:26:12.26 Toroid.in :hold @18:26:12.41 Toroid.seek :bypass @18:26:16.04 Toroid.in :default @18:26:16.16 Toroid.seek :clear @18:26:19.79 Sampled 100.0ml @18:26:21.59 SPR.command "read_sequences" @18:26:21.87 SPR.command "stats_on" @18:26:22.06 SPR.command "start 0 1" @18:26:22.77 SPR.start /var/log/esp/spr26jul3118h.spr.bz2 @18:26:22.97 Gate.power :sampler,:OFF @18:26:24.73 Handoff.select :waste @18:26:25.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:26:31.97 Syringe tare is 51% of full scale at 28C (drifted -0%) @18:26:32.03 PinPort.in :locking @18:26:32.13 PinPort.seek :locked @18:26:33.02 Archive.in :fast @18:26:33.17 Lysis2.in :fast @18:26:33.22 Lysis1.seek 1250uL @18:26:33.29 Archive.seek :empty @18:26:34.23 Lysis2.seek 950uL @18:26:42.67 Evacuating puck with filter bubble point of 20psi @18:26:42.71 PinPort.seek :locked @18:26:42.87 Handoff.select :waste @18:26:42.91 Plunge.seek :puckBottom @18:26:44.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:26:50.91 Air syringe pumping up to 1 strokes to reach 4psi @18:26:50.93 Air stroke #1 of 1 @18:26:51.09 Air.in :pressurize @18:26:51.19 Air.seek :pressurized @18:26:56.54 0.3psi after pressurize @18:26:56.57 Air.in :compress @18:26:56.70 Air.seek :evac @18:26:59.83 Reached 0.0 of 4.0psi after pumping 1572uL (0.89 strokes) @18:26:59.86 Air.in :default @18:26:59.99 Air.hold @18:27:00.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:27:11.22 Stabilized at -0.1psi after evac to waste @18:27:11.23 Handoff.select :blocked @18:27:11.80 Plunge.seek :puckTop @18:27:12.74 Air syringe pumping up to 8 strokes to reach 20psi @18:27:12.76 Air stroke #1 of 8 @18:27:12.94 Air.in :compress @18:27:13.04 Air.seek :evac @18:27:16.84 5.9psi after compress @18:27:28.94 9.7psi after compress (stable) @18:27:29.00 Air.in :gulp @18:27:29.11 Air.seek :topVent @18:27:35.61 9.4psi after gulp @18:27:41.71 9.4psi after gulp (stable) @18:27:41.72 Air stroke #2 of 8 @18:27:41.92 Air.in :pressurize @18:27:42.03 Air.seek :pressurized @18:27:46.40 Reached 9.0 of 20.0psi after pumping 2690uL (1.52 strokes) @18:27:46.42 Air.in :default @18:27:46.56 Air.hold @18:27:46.72 Plunge.seek :puckBottom @18:27:47.63 delay 3 seconds @18:27:50.66 Handoff.select :waste @18:27:51.20 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:27:58.80 delay 1:00 @18:28:58.82 Handoff.select :blocked @18:28:59.43 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @18:29:22.03 Handoff.select :waste @18:29:22.60 0.1psi at evacEnd @18:29:22.65 Plunge.seek :pTop @18:29:24.22 delay 5 seconds @18:29:29.25 Air.in :glide @18:29:29.35 Air.seek :fullVent @18:29:38.73 delay 2 seconds @18:29:40.77 Plunge.seek :pBottom @18:29:41.65 Seawater evacuated, Pressure above/below filter released. @18:29:41.68 Plunge.seek :locked @18:29:43.06 Handoff.select :waste @18:29:43.21 Lysis1.in :slow @18:29:43.30 Lysis1.seek 950uL @18:29:57.42 delay 5 seconds @18:30:02.45 Handoff.select :blocked @18:30:03.00 Plunge.seek :puckTop @18:30:04.02 Lysis1.seek 350uL @18:30:32.11 delay 3 seconds @18:30:35.21 Air.in mix with 2.79934 parts Lysis1 @18:30:36.14 Air.seek :evac @18:30:36.30 Lysis1.seek 190uL @18:30:43.98 Air.in :fast @18:30:44.09 Air.seek :full @18:30:50.65 Lysis1.seek :empty @18:30:59.81 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @14.5psi @18:30:59.84 Plunge.seek :puckBottom @18:31:00.91 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @11.0psi @18:31:00.93 Polling Can every 2:00 @18:31:01.53 PuckHeater.configure :default #ambient 28.2C @18:31:01.62 PuckHeater.seek 81.3C @18:32:22.65 PuckHeater.hold 2:00,81.3C @18:34:22.45 Polling Can every 2:00 @18:34:23.00 PuckHeater.configure :default #ambient 28.3C @18:34:23.11 PuckHeater.seek 80.3C @18:34:23.33 PuckHeater.hold 2:00,80.3C @18:36:23.16 Polling Can every 2:00 @18:36:23.71 PuckHeater.configure :default #ambient 28.3C @18:36:23.83 PuckHeater.hold 8:00,79.2C @18:44:22.40 Lysate cooked @6.5psi @18:44:22.43 Plunge.seek :puckTop @18:44:23.41 Air syringe pumping up to 5 strokes to reach 40psi @18:44:23.42 Air stroke #1 of 5 @18:44:23.63 Air.in :pressurize @18:44:23.76 Air.seek :pressurized @18:44:30.21 6.3psi after pressurize @18:44:30.29 Air.in :compress @18:44:30.42 Air.seek :evac @18:44:36.66 Reached 41.3 of 40.0psi after pumping 1749uL (0.99 strokes) @18:44:36.73 Air.in :default @18:44:36.86 Air.hold @18:44:37.00 delay 2 seconds @18:44:39.03 Air.in :fast @18:44:39.13 Air.seek :full @18:44:46.16 Plunge.seek :puckBottom @18:44:47.31 Lysis1.seek 138uL @18:44:53.91 delay 2 seconds @18:44:55.94 Plunge.seek :puckTop @18:44:56.86 Lysis1.seek :empty @18:45:03.47 Plunge.seek :puckBottom @18:45:04.63 Lysis1.in :extract @18:45:04.78 Lysis1.seek 675uL,nil @18:45:08.52 Air.in :pressurize @18:45:08.65 Air.seek :pressurized @18:45:15.12 11.3psi after pressurize @18:45:15.16 Air.in :compressLysate @18:45:15.29 Air.seek :evac @18:45:34.07 Reached 15.5 of 15.5psi after pumping 1671uL (0.94 strokes) @18:45:34.09 Air.in :default @18:45:34.21 Air.hold @18:45:35.44 Pulled 675uL lysate @15.4psi @18:45:35.69 Lysis1.seek 800uL,nil @18:45:37.08 Air.in :compressLysate @18:45:37.19 Air.seek :evac @18:45:38.51 Air.in :default @18:45:38.62 Air.hold @18:45:38.95 Air.in :glide @18:45:39.05 Air.seek :fullVent @18:45:41.65 Handoff.select :waste @18:45:42.18 Recovered 800uL lysate @13.6psi @18:45:42.20 delay 3 seconds @18:45:45.32 After 3 delay Recovered 800uL lysate @0.1psi @18:45:45.35 Plunge.seek :locked @18:45:46.74 Lysis2.in :DAmix @18:45:46.92 Lysis1.in :DAmix @18:45:47.83 Lysis2.seek 700uL @18:45:47.89 Lysis1.seek 550uL @18:46:22.97 delay 5 seconds @18:46:27.99 Handoff.select SPR @18:46:28.53 SPR.command "start 2 14" @18:46:29.71 SPR stabilized @18:46:29.92 delay 20 seconds @18:46:49.94 SPR.command "start 2 0" @18:46:52.54 Waiting up to 11:00 for SPR to enter state 1 @18:48:50.22 SPR.command "hold_on" @18:48:51.63 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:48:52.56 Lysis2.seek 400uL @18:48:52.62 Lysis1.seek 250uL @18:49:34.67 delay 15 seconds @18:49:49.71 SPR.command "hold_off" @18:49:52.25 Waiting up to 1:00:00 for SPR to enter state 7 @19:06:07.83 SPRsummary:-18.8RIU,182RIU,239RIU,309RIU,none,none,none after 2519s @19:12:51.73 -> Head.psi -0.0242973208270132 @19:12:55.35 -> Head.counts 217 @19:14:01.78 -> Power.sampler :ON @19:14:01.81 Gate.power :sampler,:ON true @19:14:12.65 -> Head.psi 3.48104320037251 @19:14:33.57 -> head.psi @19:14:33.61 NoMethodError in nadia -- undefined method `psi' for :head:Symbol @19:14:50.25 -> Head.psi 4.46616506475631 @19:15:02.24 -> Head.psi 0.534320574339236 @19:15:21.27 -> Head.psi 1.1536156264165 @19:16:11.95 -> Head.psi 4.06453167438891 @19:16:21.07 -> Head.psi 3.86909478526854 @19:16:28.34 -> Head.counts 23698 @19:16:57.95 -> d=0;30.times{d+=Head.counts};(d/30.0).round 23423 @19:17:39.86 -> d=0;30.times{d+=Head.counts};(d/30.0).round 15141 @19:18:08.71 -> d=0;30.times{d+=Head.counts};(d/30.0).round 9546 @19:18:36.34 -> d=0;30.times{d+=Head.counts};(d/30.0).round 4058 @19:18:57.14 -> d=0;30.times{d+=Head.counts};(d/30.0).round 1613 @19:19:11.36 -> Power.sampler :OFF @19:19:11.39 Gate.power :sampler,:OFF false @19:19:19.06 -> Power.sampler :ON @19:19:19.08 Gate.power :sampler,:ON true @19:19:40.10 -> d=0;30.times{d+=Head.counts};(d/30.0).round 3087 @19:19:54.66 -> d=0;30.times{d+=Head.counts};(d/30.0).round 1312 @19:20:08.10 -> d=0;30.times{d+=Head.counts};(d/30.0).round 1433 @19:20:18.81 -> d=0;30.times{d+=Head.counts};(d/30.0).round 1413 @19:20:24.74 SPR analysis completed @19:20:30.50 Ended log spr26jul3118h.spr.bz2 @19:20:30.53 SPR.command "stats_off" @19:20:31.02 -> Power.sampler :OFF @19:20:31.06 Gate.power :sampler,:OFF @19:20:31.15 Handoff.select :close false @19:20:31.74 Gate.power :heat,:OFF @19:20:31.76 Gate.power :cartridge,:OFF @19:20:31.92 PinPort.in :unlocking @19:20:32.01 PinPort.seek :clear @19:20:32.87 Carousel.in :default @19:20:33.04 Carousel.home.seek 40 @19:20:33.42 Plunge.seek :locked @19:20:33.65 Archive.in :unlocking @19:20:33.82 Archive.seek :clear @19:20:33.96 Air.in :unlocking @19:20:34.14 Air.seek :clear @19:20:34.26 Lysis1.in :unlocking @19:20:35.14 Lysis1.seek :clear @19:20:35.33 Lysis2.in :unlocking @19:20:35.43 Lysis2.seek :clear @19:20:39.54 Gate.power :motor,:OFF @19:20:39.57 Gate.power :analytic1,:OFF @19:20:39.63 Gate.power :core,:OFF @19:20:39.68 Gate.power :main,:OFF @19:20:39.72 Safely stopped and ready to power off @19:20:48.06 -> d=0;30.times{d+=Head.counts};(d/30.0).round @19:20:48.16 BusOff in nadia -- core bus was not powered while reading Intake Head Pressure's status @19:20:55.86 -> d=0;30.times{d+=Head.counts};(d/30.0).round @19:20:55.92 BusOff in nadia -- core bus was not powered while reading Intake Head Pressure's status @19:21:07.86 -> ESP.configure @19:21:07.93 Gate.power :sampler,:OFF,limit to 3500mA @19:21:07.97 Gate.power :skimmer,:OFF,limit to 3500mA @19:21:08.02 Gate.power :main,:ON,limit to 5000mA @19:21:08.08 Gate.power :analytic1,:ON,limit to 4000mA @19:21:08.19 Powering core bus @19:21:10.54 SPR Module 1, FW Rev. B, FPGA Rev. C @19:21:10.99 Plunge.configure :default @19:21:11.12 SamplePump.configure :default @19:21:11.24 Toroid.configure :default @19:21:11.35 Carousel.configure :default @19:21:11.48 Abs.configure :default @19:21:11.59 PhaseSeparator.configure :full @19:21:11.72 Archive.configure :default @19:21:11.84 Air.configure :default @19:21:11.92 Lysis1.configure :default @19:21:12.02 Lysis2.configure :default @19:21:12.13 PinPort.configure :default @19:21:12.22 All elf controllers running firmware version 4.94 @19:21:12.26 Gate.power :motor,:ON,limit to 5000mA @19:21:15.46 -> d=0;30.times{d+=Head.counts};(d/30.0).round 284 @19:21:26.58 -> d=0;30.times{d+=Head.counts};(d/30.0).round 292 @19:21:32.49 -> d=0;30.times{d+=Head.counts};(d/30.0).round 293 @19:21:41.26 -> d=0;30.times{d+=Head.counts};(d/30.0).round 262 @19:23:50.58 -> d=0;30.times{d+=Head.counts};(d/30.0).round 332 @19:23:59.38 -> d=0;30.times{d+=Head.counts};(d/30.0).round 364 @19:24:04.98 -> d=0;30.times{d+=Head.counts};(d/30.0).round 333 @19:24:13.93 -> d=0;30.times{d+=Head.counts};(d/30.0).round 349 @19:24:45.21 -> d=0;30.times{d+=Head.counts};(d/30.0).round 330 @19:29:58.97 -> MainThread.exit # @19:29:59.20 ThreadError in nadia -- killed thread @19:29:59.29 Session Ended @19:29:59.34 Session Ended @19:30:10.96EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @19:30:13.36 >BREAK< ESP3 Gateway v1.44 for core bus @19:30:13.39 >BREAK< ESP3 Gateway v1.44 for cartridge bus @19:30:17.00 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @19:30:17.68 Sea pressure (Abs) tare is 6% of full scale @19:30:21.46 Polling Can every 2:00 @19:30:21.50 Waiting for espclients... @19:30:22.65 Session Begun @19:30:32.75 -> ESP.ready! @19:30:32.93 Gate.power :sampler,:OFF,limit to 3500mA @19:30:32.95 Gate.power :skimmer,:OFF,limit to 3500mA @19:30:32.99 Gate.power :analytic1,:ON,limit to 4000mA @19:30:33.77 Plunge.configure :default @19:30:33.94 SamplePump.configure :default @19:30:34.10 Toroid.configure :default @19:30:34.25 SPR Module 1, FW Rev. B, FPGA Rev. C @19:30:34.40 Carousel.configure :default @19:30:34.57 Abs.configure :default @19:30:34.73 PhaseSeparator.configure :default @19:30:34.90 Archive.configure :default @19:30:35.07 Air.configure :default @19:30:35.21 Lysis1.configure :default @19:30:35.34 Lysis2.configure :default @19:30:35.50 PinPort.configure :default @19:30:35.59 All elf controllers running firmware version 4.94 @19:30:35.84 Plunge.in :default @19:30:36.40 Plunge.home.jog 0 @19:30:36.63 PinPort.in :default @19:30:36.71 Lysis1.in :default @19:30:36.75 Air.in :default @19:30:36.79 Carousel.in :default @19:30:36.85 Toroid.in :default @19:30:36.91 Lysis2.in :default @19:30:37.01 Archive.in :default @19:30:37.26 PinPort.home.jog 0 @19:30:37.38 Lysis1.home.jog 0 @19:30:37.42 Air.home.jog 0 @19:30:37.51 Lysis2.home.jog 0 @19:30:37.55 Archive.home.jog 0 @19:30:37.60 Carousel.home.jog 0 @19:30:37.63 Toroid.home.jog 0 @19:30:38.43 Plunge.in :homing @19:30:38.79 Air.in :homing @19:30:38.90 Plunge.jog 1483 @19:30:38.94 Lysis2.in :homing @19:30:38.99 Toroid.in :homing @19:30:39.03 Lysis1.in :homing @19:30:39.07 Archive.in :homing @19:30:39.11 PinPort.in :homing @19:30:39.95 Air.jog 1427 @19:30:40.02 Lysis1.jog 1596 @19:30:40.04 Toroid.jog 1352 @19:30:40.09 Lysis2.jog 1617 @19:30:40.14 Archive.jog 1614 @19:30:40.18 PinPort.jog 1232 @19:30:40.66 Plunge.setRawPosition! 50 @19:30:40.73 Plunge.seek :home @19:30:41.03 Plunge.in :default @19:30:41.41 Air.setRawPosition! 0 @19:30:41.49 Air.seek :home @19:30:41.63 Lysis1.setRawPosition! 50 @19:30:41.71 Lysis1.seek :home @19:30:41.88 Air.in :default @19:30:41.91 Lysis2.setRawPosition! 50 @19:30:41.94 Archive.setRawPosition! 50 @19:30:42.11 Lysis2.seek :home @19:30:42.15 Archive.seek :home @19:30:42.41 PinPort.setRawPosition! 3150 @19:30:42.44 Lysis1.in :default @19:30:42.60 PinPort.seek :home @19:30:42.75 Archive.in :default @19:30:42.79 Lysis2.in :default @19:30:44.91 Toroid.setRawPosition! 0 @19:30:44.98 Toroid.seek :home @19:30:45.10 Toroid.in :default @19:30:47.01 PinPort.in :default @19:30:47.37 Toroid.seek :clear @19:30:47.56 PinPort.in :unlocking @19:30:47.64 PinPort.seek :clear @19:30:51.01 Air.seek :clear @19:30:51.14 Lysis1.seek :clear @19:30:51.87 Lysis2.seek :clear @19:30:52.69 Archive.seek :clear @19:30:53.49 Carousel.in :default @19:30:53.64 Carousel.home.seek 40 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.58psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @19:30:56.10 -> Head.psi 0.589071031423269 @19:31:03.46 -> Head.psi 0.583349303001137 @19:31:06.21 -> Head.psi 0.583967868235962 @19:31:10.82 -> Head.counts 302 @19:31:34.90 -> Head.counts 260 @19:32:07.71 -> MainThread.exit @19:32:07.82 Intake.close @19:32:07.92 Exhaust.close @19:32:07.96 Bypass.close # @19:32:08.36 Session Ended @19:32:19.04EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @19:32:20.88 >BREAK< ESP3 Gateway v1.44 for core bus @19:32:20.91 >BREAK< ESP3 Gateway v1.44 for cartridge bus @19:32:24.54 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @19:32:25.21 Sea pressure (Abs) tare is 6% of full scale @19:32:28.91 Polling Can every 2:00 @19:32:28.96 Waiting for espclients... @19:32:30.12 Session Begun @19:32:31.70 -> ESP.ready! @19:32:31.88 Gate.power :sampler,:OFF,limit to 3500mA @19:32:31.90 Gate.power :skimmer,:OFF,limit to 3500mA @19:32:31.94 Gate.power :analytic1,:ON,limit to 4000mA @19:32:32.55 SPR Module 1, FW Rev. B, FPGA Rev. C @19:32:32.76 Plunge.configure :default @19:32:32.92 SamplePump.configure :default @19:32:33.10 Toroid.configure :default @19:32:33.18 Toroid.hold @19:32:33.28 Toroid.configure :default @19:32:33.44 Carousel.configure :default @19:32:33.53 Carousel.hold @19:32:33.62 Carousel.configure :default @19:32:33.78 Abs.configure :default @19:32:33.94 PhaseSeparator.configure :default @19:32:34.11 Archive.configure :default @19:32:34.20 Archive.hold @19:32:34.34 Archive.configure :default @19:32:34.51 Air.configure :default @19:32:34.60 Air.hold @19:32:34.70 Air.configure :default @19:32:34.85 Lysis1.configure :default @19:32:34.93 Lysis1.hold @19:32:35.04 Lysis1.configure :default @19:32:35.20 Lysis2.configure :default @19:32:35.29 Lysis2.hold @19:32:35.39 Lysis2.configure :default @19:32:35.56 PinPort.configure :default @19:32:35.65 PinPort.hold @19:32:35.75 PinPort.configure :default @19:32:35.83 All elf controllers running firmware version 4.94 @19:32:36.67 Toroid.seek :clear @19:32:36.88 Air.seek :clear @19:32:37.00 Lysis1.seek :clear @19:32:37.11 Lysis2.seek :clear @19:32:37.24 Archive.seek :clear @19:32:37.35 Carousel.in :default @19:32:37.92 Carousel.home.seek 40 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.58psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @19:32:41.37 -> Head.psi 0.569050306529787 @19:32:49.61 -> Power.sampler :ON @19:32:49.65 Gate.power :sampler,:ON true @19:33:04.25 -> Head.psi 0.732678941294786 @19:33:15.77 -> Head.psi 0.926929948161363 @19:33:25.29 -> Head.psi 2.14007079040773 @19:33:33.37 -> Head.psi 4.17228275714883 @19:33:41.55 -> Power.sampler :OFF @19:33:41.57 Gate.power :sampler,:OFF false @19:36:29.79 -> slot {20 => { Type: :microSPR_phyto, State: :dry}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :dry}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @19:39:50.91 -> #slot 40 (L27) & slot 50/51 (A53) cool L.Huron water +di 50/50 @19:40:37.86 -> slot 30=>:lyseHold_phyto {30 => { Type: :lyseHold_phyto, State: :dry}} @19:41:10.90 -> #L. Huron microsystin water now being used @19:41:29.60 -> #SC 30 has L72 @19:41:34.89 -> slot {20 => { Type: :microSPR_phyto, State: :dry}, 30 => { Type: :lyseHold_phyto, State: :dry}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :dry}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @19:43:18.51 -> # SC45 A33 @19:44:18.48 -> Cart.unlock @19:44:18.58 Carousel.in :default @19:44:18.73 Carousel.home.seek 40 @19:44:19.37 Plunge.in :unlocking @19:44:19.46 Plunge.seek :clear @19:44:22.84 -> SC.to 20 @19:44:22.87 Carousel.seek 20 Storage Carousel (SC) at 20 @19:44:48.82 -> #SC20 L79 @19:44:56.97 -> Cart.dry [45,30,20] @19:45:23.70 -> MainThread.exit @19:45:23.81 Intake.close @19:45:23.89 Exhaust.close @19:45:23.94 Bypass.close # @19:45:24.35 Session Ended @19:45:36.73EDT31-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @19:45:38.46 >BREAK< ESP3 Gateway v1.44 for core bus @19:45:38.49 >BREAK< ESP3 Gateway v1.44 for cartridge bus @19:45:42.03 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @19:45:42.74 Sea pressure (Abs) tare is 6% of full scale @19:45:46.42 Polling Can every 2:00 @19:45:46.46 Waiting for espclients... @19:45:47.86 Session Begun @19:48:36.82 -> start {fire lysis, archive, hold} # @19:48:37.18 Gate.power :sampler,:OFF,limit to 3500mA @19:48:37.21 Gate.power :skimmer,:OFF,limit to 3500mA @19:48:37.25 Gate.power :analytic1,:ON,limit to 4000mA @19:48:37.98 Plunge.configure :default @19:48:38.07 Plunge.hold @19:48:38.17 SPR Module 1, FW Rev. B, FPGA Rev. C @19:48:38.24 Plunge.configure :default @19:48:38.43 SamplePump.configure :default @19:48:38.60 Toroid.configure :default @19:48:38.69 Toroid.hold @19:48:38.79 Toroid.configure :default @19:48:38.95 Carousel.configure :default @19:48:39.04 Carousel.hold @19:48:39.14 Carousel.configure :default @19:48:39.31 Abs.configure :default @19:48:39.48 PhaseSeparator.configure :default @19:48:39.65 Archive.configure :default @19:48:39.74 Archive.hold @19:48:39.84 Archive.configure :default @19:48:40.01 Air.configure :default @19:48:40.09 Air.hold @19:48:40.20 Air.configure :default @19:48:40.38 Lysis1.configure :default @19:48:40.47 Lysis1.hold @19:48:40.58 Lysis1.configure :default @19:48:40.71 Lysis2.configure :default @19:48:40.80 Lysis2.hold @19:48:40.90 Lysis2.configure :default @19:48:41.06 PinPort.configure :default @19:48:41.15 PinPort.hold @19:48:41.26 PinPort.configure :default @19:48:41.34 All elf controllers running firmware version 4.94 @19:48:42.23 Toroid.seek :clear @19:48:42.48 Air.seek :clear @19:48:42.98 Lysis1.seek :clear @19:48:43.10 Lysis2.seek :clear @19:48:43.22 Archive.seek :clear @19:48:43.34 Carousel.in :default @19:48:43.50 Carousel.home.seek 20 @19:48:43.86 Selecting Cartridge 20 @19:48:44.20 Carousel.seek 20 @19:48:44.56 Carousel.reconfigure :align @19:48:44.73 Lysis1.in :fast @19:48:44.81 Lysis1.seek :locked @19:48:46.25 Slide::Warning in core -- Storage Carousel (SC) reseek at 20 - 5 counts (sensed 20 - 4 counts) @19:48:47.48 Plunge.seek :locked @19:48:47.68 Air.in :fast @19:48:47.77 Air.seek :locked @19:48:48.85 Carousel.reconfigure :locked @19:48:49.03 Powering cartridge bus @19:48:51.70 Gate.setIR 1750,5000,0,6 @19:48:51.84 Gate.setIR 0,5000,0,6 @19:48:51.94 PuckHeater.configure :default @19:48:52.03 Gate.power :heat,:ON,limit to 5000mA @19:48:54.27 Mounted lysis Cartridge board#J59971 @19:48:54.37 Using default cartridge heat loss curve for HeatingFunctionInCarousel @19:48:54.71 Preparing to filter microSPR_phyto cartridge in slot 20 @19:48:55.00 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @19:49:01.63 Intake Head Pressure tare is 1% of full scale at 29C (drifted 6%) @19:49:01.65 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @19:49:01.71 PhaseSeparator.in :filling @19:49:01.79 PhaseSeparator.hold @19:49:01.89 Gate.power :sampler,:ON @19:49:08.62 Head pressure reached 3.95psi in 7 seconds @19:49:08.67 Air.in :default @19:49:08.83 Air.seek :sealed @19:49:08.88 Priming sample loop w/200ml, bypass w/10ml @19:49:08.97 SamplePump.setPosition! 0ml @19:49:09.08 Exhaust.close @19:49:09.19 Bypass.open @19:49:11.98 Intake.open @19:49:13.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @19:49:16.35 Vacuum tare is 54% of full scale at 29C (drifted 2%) @19:49:16.55 Abs.in :default @19:49:16.63 Abs.hold @19:49:16.74 PhaseSeparator.in :full @19:49:16.82 PhaseSeparator.hold @19:49:17.09 Waiting for sampler to finish priming... @19:49:17.11 delayUntil "19:50:01.91" @19:50:01.97 SamplePump.in :start @19:50:02.07 SamplePump.seek 10ml,1:10 @19:50:02.67 SamplePump.configure :prime @19:50:15.61 Abs.stop @19:50:15.69 PhaseSeparator.stop @19:50:15.78 SamplePump.setPosition! 0ml @19:50:15.85 Exhaust.open @19:50:18.13 Bypass.close @19:50:20.57 Abs.in :default @19:50:20.65 Abs.hold @19:50:20.76 PhaseSeparator.hold @19:50:21.10 SamplePump.in :start @19:50:21.20 SamplePump.seek 200ml,13:50 @19:50:21.80 SamplePump.configure :prime @19:54:43.95 Abs.stop @19:54:44.03 PhaseSeparator.stop @19:54:44.13 Bypass.open @19:54:45.67 Intake.close @19:54:47.27 Toroid.in :hold @19:54:47.35 Toroid.seek :crack @19:54:49.86 Toroid.seek :cartridge @19:54:52.01 Toroid.in :default @19:54:52.10 Toroid.seek :clear @19:54:56.10 Pressurizing cartridge to 14psi above 15.4psia @19:54:56.12 SamplePump.setPosition! 0ml @19:54:56.22 Intake.open @19:54:57.72 Bypass.close @19:54:59.27 Exhaust.close @19:55:00.98 Abs.in :default @19:55:01.06 Abs.hold @19:55:01.16 PhaseSeparator.hold @19:55:01.85 SamplePump.in :charge @19:55:02.03 SamplePump.seek 2.3ml,38 seconds @19:55:13.34 Abs.stop @19:55:13.43 PhaseSeparator.stop @19:55:13.53 Intake.close @19:55:15.21 Pressurized cartridge with 0.526ml to 44.7psi @19:55:15.23 Isolated @23.2psia (7.9psi) @19:55:15.28 Verifying cartridge holds at least 0.65..0.85 of 23.2psia @19:55:15.34 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @19:55:20.03 Sampling 100.0ml @19:55:20.05 SamplePump.setPosition! 0ml @19:55:20.17 Exhaust.open @19:55:21.71 Intake.open @19:55:23.42 Abs.in :default @19:55:23.50 Abs.hold @19:55:23.63 PhaseSeparator.hold @19:55:24.02 SamplePump.in :start @19:55:24.12 SamplePump.seek 100ml,6:04 @19:55:24.70 SamplePump.configure :sample @19:55:27.13 Cartridge 20 filtering 2/100ml at 0.72ml/s with 9.3psi vacuum 5.3m deep (22.6psia) @19:56:27.19 Cartridge 20 filtering 39/100ml at 0.63ml/s with 7.4psi vacuum 4.9m deep (21.9psia) @19:57:27.18 Cartridge 20 filtering 72/100ml at 0.55ml/s with 12.5psi vacuum @19:58:24.99 Abs.stop @19:58:25.07 PhaseSeparator.stop @19:58:25.18 Intake.close @19:58:25.28 Exhaust.close @19:58:25.52 Toroid.in :hold @19:58:25.61 Toroid.seek :bypass @19:58:29.22 Toroid.in :default @19:58:29.31 Toroid.seek :clear @19:58:32.91 Sampled 100.0ml @19:58:34.60 SPR.command "read_sequences" @19:58:34.89 SPR.command "stats_on" @19:58:35.06 SPR.command "start 0 1" @19:58:35.77 SPR.start /var/log/esp/spr26jul3119h.spr.bz2 @19:58:36.07 Gate.power :sampler,:OFF @19:58:38.29 Handoff.select :waste @19:58:39.41 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:58:46.06 Syringe tare is 50% of full scale at 29C (drifted -0%) @19:58:46.11 PinPort.in :locking @19:58:46.20 PinPort.seek :locked @19:58:47.08 Archive.in :fast @19:58:47.15 Lysis2.in :fast @19:58:47.21 Lysis1.seek 1250uL @19:58:47.42 Lysis2.seek 950uL @19:58:47.48 Archive.seek :empty @19:58:56.04 Evacuating puck with filter bubble point of 20psi @19:58:56.08 PinPort.seek :locked @19:58:56.23 Handoff.select :waste @19:58:56.29 Plunge.seek :puckBottom @19:58:57.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:59:04.27 Air syringe pumping up to 1 strokes to reach 4psi @19:59:04.31 Air stroke #1 of 1 @19:59:04.45 Air.in :pressurize @19:59:04.55 Air.seek :pressurized @19:59:09.84 0.1psi after pressurize @19:59:09.87 Air.in :compress @19:59:09.97 Air.seek :evac @19:59:16.62 1.8psi after compress @19:59:30.71 0.3psi after compress (stable) @19:59:30.73 Air.in :default @19:59:30.89 Air.hold @19:59:31.20 at 0.4psi Opened to waste @19:59:31.24 Handoff.select :blocked @19:59:31.77 Plunge.seek :puckTop @19:59:32.69 Air syringe pumping up to 8 strokes to reach 20psi @19:59:32.70 Air stroke #1 of 8 @19:59:32.84 Air.in :compress @19:59:32.92 Air.seek :evac @19:59:33.14 1.9psi after compress @19:59:43.74 2.1psi after compress (stable) @19:59:43.77 Air.in :gulp @19:59:43.87 Air.seek :topVent @19:59:50.39 2.0psi after gulp @19:59:56.49 2.2psi after gulp (stable) @19:59:56.50 Air stroke #2 of 8 @19:59:56.65 Air.in :pressurize @19:59:56.74 Air.seek :pressurized @20:00:03.18 2.3psi after pressurize @20:00:03.21 Air.in :compress @20:00:03.31 Air.seek :evac @20:00:10.03 13.4psi after compress @20:00:23.14 16.3psi after compress (stable) @20:00:23.17 Air.in :gulp @20:00:23.31 Air.seek :topVent @20:00:29.81 16.2psi after gulp @20:00:35.44 16.3psi after gulp (stable) @20:00:35.45 Air stroke #3 of 8 @20:00:35.62 Air.in :pressurize @20:00:35.71 Air.seek :pressurized @20:00:42.13 15.9psi after pressurize @20:00:42.18 Air.in :compress @20:00:42.31 Air.seek :evac @20:00:47.21 Reached 20.4 of 20.0psi after pumping 5223uL (2.94 strokes) @20:00:47.23 Air.in :default @20:00:47.33 Air.hold @20:00:47.44 Plunge.seek :puckBottom @20:00:48.35 delay 3 seconds @20:00:51.37 Handoff.select :waste @20:00:51.90 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:01:02.51 delay 1:00 @20:02:02.53 Handoff.select :blocked @20:02:03.15 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @20:02:25.74 Handoff.select :waste @20:02:26.25 0.3psi at evacEnd @20:02:26.29 Plunge.seek :pTop @20:02:27.23 delay 5 seconds @20:02:32.26 Air.in :glide @20:02:32.39 Air.seek :fullVent @20:02:35.12 delay 2 seconds @20:02:37.15 Plunge.seek :pBottom @20:02:38.04 Seawater evacuated, Pressure above/below filter released. @20:02:38.07 Plunge.seek :locked @20:02:39.44 Handoff.select :waste @20:02:39.60 Lysis1.in :slow @20:02:39.68 Lysis1.seek 950uL @20:02:53.79 delay 5 seconds @20:02:58.81 Handoff.select :blocked @20:02:59.80 Plunge.seek :puckTop @20:03:00.80 Lysis1.seek 350uL @20:03:01.79 Lysis1.setRawPosition! -4926 @20:03:01.88 Slide::Error in PROCESSING -- Lysis Syringe #1 (LyS1) positionErr at 935uL (sensed 958uL) @20:03:01.90 Waiting to Resume from Checkpoint "Lysis Syringe #1 (LyS1) positionErr at 935uL (sensed 958uL)" @20:13:07.49 Ended log spr26jul3119h.spr.bz2 @20:13:07.51 SPR.command "stats_off" @22:34:17.90 Session Begun @22:34:26.10 -> Lysis1 Lysis Syringe #1 (LyS1) at 957uL @22:34:44.02 -> Lysis1.home.jog 0 @22:34:44.06 Lysis1.in :default @22:34:44.18 Lysis1.home.jog 0 Lysis Syringe #1 (LyS1) at 958uL @22:34:56.10 -> Handoff.blocked @22:34:56.13 Handoff.select :blocked Handoff Valve::Manifold selects blocked @22:35:20.50 -> Lysis1.to 350, :slow @22:35:20.56 Lysis1.in :slow @22:35:20.67 Lysis1.seek 350uL Lysis Syringe #1 (LyS1) at 350uL @22:37:22.13 -> Air.in :slow do @22:37:22.16 SyntaxError in preston#2 -- compile error preston#2:5: syntax error, unexpected $end Air.in :slow do ^ @22:37:22.38 -> Syringe.mix Air=>Air.volume-Air.at(:evac).volume, @22:37:22.43 SyntaxError in preston#2 -- compile error preston#2:6: syntax error, unexpected $end Syringe.mix Air=>Air.volume-Air.at(:evac).volume, ^ @22:37:22.62 -> syr=>160 #helps seat check valve @22:37:22.68 SyntaxError in preston#2 -- compile error preston#2:7: syntax error, unexpected tASSOC, expecting $end syr=>160 #helps seat check valve ^ @22:37:25.30 -> end @22:37:25.33 SyntaxError in preston#2 -- compile error preston#2:8: syntax error, unexpected kEND @22:37:46.08 -> Air.reconfigure :slow @22:37:46.13 Air.in :slow Air Syringe (AirS) at fVent @22:38:34.42 -> Syringe.mix Air=>Air.volume-Air.at(:evac).volume, Lysis1=>160 @22:38:34.57 Air.in mix with 2.79934 parts Lysis1 @22:38:34.72 Lysis1.in :default @22:38:35.42 Air.seek :evac @22:38:35.48 Lysis1.seek 190uL Archive = Archive Syringe (ArS) empty Air = Air Syringe (AirS) at evac Lysis1 = Lysis Syringe #1 (LyS1) at 190uL Lysis2 = Lysis Syringe #2 (LyS2) at 950uL @22:38:55.38 -> Air.fill :fast @22:38:55.45 Air.in :fast @22:38:55.54 Air.seek :full Air Syringe (AirS) full @22:39:06.10 -> Lysis1.empty @22:39:06.14 Lysis1.seek :empty Lysis Syringe #1 (LyS1) empty @22:39:18.91 -> "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % @22:39:18.95 SyntaxError in preston#2 -- compile error preston#2:13: syntax error, unexpected $end ...} Syringe pressure @%.1fpsi" % ^ @22:39:35.30 -> "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % Syringe.pressure Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @7.7psi @22:39:45.22 -> Plunge.pBottom @22:39:45.28 Plunge.seek :pBottom Plunge Valve (PV) at puckBottom @22:39:59.78 -> "Lysis Buffer Delivered. #{Plunge.examine} Syringe pressure @%.1fpsi" % Syringe.pressure Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @6.6psi @22:42:07.36 -> padProfile @22:42:07.41 Polling Can every 2:00 @22:42:07.97 PuckHeater.configure :default #ambient 28.7C @22:42:08.07 PuckHeater.seek 81.3C @22:43:25.90 PuckHeater.hold 2:00,81.3C @22:45:25.32 Polling Can every 2:00 @22:45:25.88 PuckHeater.configure :default #ambient 28.8C @22:45:25.97 PuckHeater.seek 80.2C @22:45:26.18 PuckHeater.hold 2:00,80.2C @22:47:25.56 Polling Can every 2:00 @22:47:26.12 PuckHeater.configure :default #ambient 28.8C @22:47:26.21 PuckHeater.hold 8:00,79.2C Puck Heater at 79.4C @22:55:35.24 -> Heat Puck Heater at 75.3C @22:55:42.10 -> Power.status {analytic1: ON with current limited to 4000mA, cartridge: ON, core: ON, heat: ON with current limited to 5000mA, main: ON with current limited to 5000mA, motor: ON with current limited to 5000mA, sampler: off with current limited to 3500mA, skimmer: off with current limited to 3500mA} @22:55:51.78 -> Heat.status @22:56:03.71 -> Log.comment "Lysate cooked @%.1fpsi" % Syringe.pressure; Plunge.to :puckTop; Air.pumpTo 40, 5; delay 2; Air.fill :fast; Plunge.to :puckBottom; Lysis1.pull 138, :slow; delay 2; Plunge.pTop; Lysis1.ignoreErrTo :empty; Plunge.to :puckBottom; Cart.sampler.recoverLysate @22:56:03.84 Lysate cooked @11.7psi @22:56:03.87 Plunge.seek :puckTop @22:56:04.81 Air syringe pumping up to 5 strokes to reach 40psi @22:56:04.83 Air stroke #1 of 5 @22:56:04.98 Air.in :pressurize @22:56:05.07 Air.seek :pressurized @22:56:11.50 11.0psi after pressurize @22:56:11.53 Air.in :compress @22:56:11.65 Air.seek :evac @22:56:14.00 Reached 15.4 of 40.0psi after pumping 1528uL (0.86 strokes) @22:56:14.02 Air.in :slow @22:56:14.14 Air.hold @22:56:14.26 delay 2 seconds @22:56:16.33 Air.in :fast @22:56:16.43 Air.seek :full @22:56:22.12 Plunge.seek :puckBottom @22:56:23.10 Lysis1.in :slow @22:56:23.21 Lysis1.seek 138uL @22:56:29.79 delay 2 seconds @22:56:31.83 Plunge.seek :pTop @22:56:32.74 Lysis1.in :default @22:56:32.84 Lysis1.seek :empty @22:56:34.57 Plunge.seek :puckBottom @22:56:35.65 Lysis1.in :extract @22:56:35.79 Lysis1.seek 675uL,nil @22:56:37.80 Air.in :pressurize @22:56:37.95 Air.seek :pressurized @22:56:44.51 10.1psi after pressurize @22:56:44.54 Air.in :compressLysate @22:56:44.69 Air.seek :evac @22:56:59.58 Reached 12.8 of 15.5psi after pumping 1614uL (0.91 strokes) @22:56:59.60 Air.in :slow @22:56:59.74 Air.hold @22:57:00.21 Air.in :compressLysate @22:57:00.40 Air.seek :evac @22:57:02.91 Reached 15.5 of 15.5psi after pumping 1647uL (0.93 strokes) @22:57:02.93 Air.in :slow @22:57:03.05 Air.hold @22:57:04.06 Air.in :compressLysate @22:57:04.17 Air.seek :evac @22:57:05.53 Syringe::LysatePlungerStuck in preston#2 -- at < 585uL during Lysis Syringe #1 (LyS1) move @22:57:05.61 Lysis1.in :default @22:57:05.70 Lysis1.seek 555uL @22:57:07.03 Reached 15.2 of 15.5psi after pumping 1683uL (0.95 strokes) @22:57:07.05 Air.in :slow @22:57:07.18 Air.hold @22:57:07.58 Lysis1.in :extract @22:57:07.73 Lysis1.seek 675uL,nil @22:57:10.18 Air.hold @22:57:10.53 Pulled 675uL lysate @14.0psi @22:57:10.89 Lysis1.seek 800uL,nil @22:57:11.18 Air.in :compressLysate @22:57:11.34 Air.seek :evac @22:57:18.01 13.8psi after compress @22:57:18.35 Air.in :slow @22:57:18.45 Air.hold @22:57:18.76 Air.in :glide @22:57:18.87 Air.seek :fullVent @22:57:20.55 Handoff.select :waste @22:57:21.09 Recovered 800uL lysate @13.9psi @22:57:21.11 delay 3 seconds @22:57:24.22 After 3 delay Recovered 800uL lysate @0.9psi 800 @22:57:38.08 -> slot {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 100.0, lysed: 800, spr: spr26jul3119h.spr.bz2}, 30 => { Type: :lyseHold_phyto, State: :dry}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :dry}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @22:58:16.02 -> #SC20 was processed manually as a Lyse Hold. @22:58:37.47 -> slot 30=>:microSPR_phyto {30 => { Type: :microSPR_phyto, State: :dry}} @22:58:42.17 -> Cart.dry [45,30] @22:59:16.42 -> start {fire lysis, archive} @22:59:16.63 Selecting Cartridge 30 # @22:59:16.90 Gate.power :heat,:OFF @22:59:16.93 Gate.power :cartridge,:OFF @22:59:17.09 PinPort.in :unlocking @22:59:17.19 PinPort.seek :clear @22:59:18.06 Carousel.in :default @22:59:18.23 Carousel.home.seek 20 @22:59:18.67 Archive.in :unlocking @22:59:18.82 Archive.seek :clear @22:59:18.99 Air.in :unlocking @22:59:19.19 Air.seek :clear @22:59:19.27 Lysis2.in :unlocking @22:59:19.47 Lysis2.seek :clear @22:59:24.11 Lysis1.in :unlocking @22:59:24.27 Lysis1.seek :clear @22:59:24.45 Plunge.in :unlocking @22:59:24.57 Plunge.seek :clear @22:59:27.43 Carousel.seek 30 @22:59:29.94 Carousel.reconfigure :align @22:59:30.15 Lysis1.in :fast @22:59:30.31 Lysis1.seek :locked @22:59:30.46 Slide::Warning in core -- Storage Carousel (SC) reseek at 30 + 3 counts (sensed 30 + 4 counts) @22:59:32.99 Plunge.in :default @22:59:33.08 Plunge.seek :locked @22:59:33.29 Air.in :fast @22:59:33.45 Air.seek :locked @22:59:34.56 Carousel.reconfigure :locked @22:59:34.76 Powering cartridge bus @22:59:37.44 Gate.setIR 1750,5000,0,6 @22:59:37.57 Gate.setIR 0,5000,0,6 @22:59:37.67 PuckHeater.configure :default #ambient 28.8C @22:59:37.78 Gate.power :heat,:ON,limit to 5000mA @22:59:38.02 Mounted lysis Cartridge board#J59913 @22:59:38.07 Using default cartridge heat loss curve for HeatingFunctionInCarousel @22:59:38.49 Preparing to filter microSPR_phyto cartridge in slot 30 @22:59:38.80 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @22:59:45.42 Intake Head Pressure tare is 1% of full scale at 29C (drifted 6%) @22:59:45.45 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @22:59:45.55 PhaseSeparator.in :filling @22:59:45.64 PhaseSeparator.hold @22:59:45.76 Gate.power :sampler,:ON @22:59:52.49 Head pressure reached 3.82psi in 7 seconds @22:59:52.68 Air.in :slow @22:59:52.86 Priming sample loop w/200ml, bypass w/10ml @22:59:52.91 Air.seek :sealed @22:59:52.97 SamplePump.setPosition! 0ml @22:59:53.14 Bypass.open @22:59:55.50 Intake.open @22:59:57.10 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:59:59.72 Vacuum tare is 54% of full scale at 29C (drifted 2%) @22:59:59.92 Abs.in :default @23:00:00.03 Abs.hold @23:00:00.14 PhaseSeparator.in :full @23:00:00.23 PhaseSeparator.hold @23:00:00.55 Waiting for sampler to finish priming... @23:00:00.58 delayUntil "23:00:45.78" @23:00:45.94 SamplePump.in :start @23:00:46.07 SamplePump.seek 10ml,1:10 @23:00:46.63 SamplePump.configure :prime @23:00:59.64 Abs.stop @23:00:59.74 PhaseSeparator.stop @23:00:59.83 SamplePump.setPosition! 0ml @23:00:59.93 Exhaust.open @23:01:02.18 Bypass.close @23:01:04.66 Abs.in :default @23:01:04.77 Abs.hold @23:01:04.89 PhaseSeparator.hold @23:01:05.28 SamplePump.in :start @23:01:05.40 SamplePump.seek 200ml,13:50 @23:01:05.97 SamplePump.configure :prime @23:02:22.99 -> exit @23:02:23.04 Session Ended @23:05:28.02 Abs.stop @23:05:28.10 PhaseSeparator.stop @23:05:28.21 Bypass.open @23:05:29.79 Intake.close @23:05:31.35 Toroid.in :hold @23:05:31.45 Toroid.seek :crack @23:05:33.95 Toroid.seek :cartridge @23:05:36.10 Toroid.in :default @23:05:36.19 Toroid.seek :clear @23:05:40.20 Pressurizing cartridge to 14psi above 14.0psia @23:05:40.22 SamplePump.setPosition! 0ml @23:05:40.36 Intake.open @23:05:41.83 Bypass.close @23:05:43.36 Exhaust.close @23:05:45.05 Abs.in :default @23:05:45.13 Abs.hold @23:05:45.24 PhaseSeparator.hold @23:05:45.52 SamplePump.in :charge @23:05:45.61 SamplePump.seek 2.3ml,38 seconds @23:05:59.53 Abs.stop @23:05:59.63 PhaseSeparator.stop @23:06:00.35 Intake.close @23:06:01.95 Pressurized cartridge with 0.801ml to 14.9psi @23:06:01.97 Isolated @27.0psia (13.0psi) @23:06:02.02 Verifying cartridge holds at least 0.65..0.85 of 27.0psia @23:06:02.07 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:06:06.69 Cartridge pressure fell 4% to 25.9psia @23:06:06.82 Sampling 100.0ml @23:06:06.84 SamplePump.setPosition! 0ml @23:06:06.94 Exhaust.open @23:06:08.48 Intake.open @23:06:10.16 Abs.in :default @23:06:10.24 Abs.hold @23:06:10.40 PhaseSeparator.hold @23:06:10.80 SamplePump.in :start @23:06:10.89 SamplePump.seek 100ml,6:04 @23:06:11.48 SamplePump.configure :sample @23:06:13.89 Cartridge 30 filtering 2/100ml at 0.69ml/s with 14.4psi vacuum @23:07:13.95 Cartridge 30 filtering 44/100ml at 0.71ml/s with 8.9psi vacuum @23:08:13.95 Cartridge 30 filtering 82/100ml at 0.64ml/s with 11.8psi vacuum @23:08:43.47 Abs.stop @23:08:43.55 PhaseSeparator.stop @23:08:43.67 Intake.close @23:08:43.75 Exhaust.close @23:08:43.88 Handoff.select :close @23:08:44.13 Toroid.in :hold @23:08:44.21 Toroid.seek :bypass @23:08:47.86 Toroid.in :default @23:08:47.95 Toroid.seek :clear @23:08:51.55 Sampled 100.0ml @23:08:52.06 Cmd::Error in detached from preston -- Cannot load cartridge while in PROCESSING state @23:08:52.12 Waiting to Resume from Checkpoint "Selecting Cartridge 20" @23:08:52.31 SPR.command "stats_on" @23:08:53.14 SPR.command "start 0 1" @23:08:53.83 SPR.start /var/log/esp/spr26jul3123h.spr.bz2 @23:08:54.72 Gate.power :sampler,:OFF @23:08:56.41 Handoff.select :waste @23:08:56.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:09:03.56 Syringe tare is 51% of full scale at 29C (drifted -0%) @23:09:03.61 PinPort.in :locking @23:09:03.70 PinPort.seek :locked @23:09:04.57 Archive.in :fast @23:09:04.67 Lysis2.in :fast @23:09:04.75 Lysis1.seek 1250uL @23:09:04.86 Archive.seek :empty @23:09:05.03 Lysis2.seek 950uL @23:09:13.56 Evacuating puck with filter bubble point of 20psi @23:09:13.62 PinPort.seek :locked @23:09:13.74 Handoff.select :waste @23:09:13.79 Plunge.seek :puckBottom @23:09:15.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:09:21.74 Air syringe pumping up to 1 strokes to reach 4psi @23:09:21.75 Air stroke #1 of 1 @23:09:21.92 Air.in :pressurize @23:09:22.02 Air.seek :pressurized @23:09:27.31 0.3psi after pressurize @23:09:27.34 Air.in :compress @23:09:27.44 Air.seek :evac @23:09:34.09 2.5psi after compress @23:09:46.69 0.2psi after compress (stable) @23:09:46.71 Air.in :slow @23:09:46.82 Air.hold @23:09:47.06 at 0.1psi Opened to waste @23:09:47.08 Handoff.select :blocked @23:09:47.61 Plunge.seek :puckTop @23:09:48.52 Air syringe pumping up to 8 strokes to reach 20psi @23:09:48.54 Air stroke #1 of 8 @23:09:48.70 Air.in :compress @23:09:48.78 Air.seek :evac @23:09:48.98 1.3psi after compress @23:09:59.59 1.7psi after compress (stable) @23:09:59.62 Air.in :gulp @23:09:59.83 Air.seek :topVent @23:10:06.46 1.6psi after gulp @23:10:12.11 1.7psi after gulp (stable) @23:10:12.14 Air stroke #2 of 8 @23:10:12.29 Air.in :pressurize @23:10:12.41 Air.seek :pressurized @23:10:18.82 2.2psi after pressurize @23:10:18.85 Air.in :compress @23:10:18.96 Air.seek :evac @23:10:25.61 13.5psi after compress @23:10:37.21 15.8psi after compress (stable) @23:10:37.24 Air.in :gulp @23:10:37.34 Air.seek :topVent @23:10:43.83 15.1psi after gulp @23:10:52.42 15.1psi after gulp (stable) @23:10:52.44 Air stroke #3 of 8 @23:10:52.59 Air.in :pressurize @23:10:52.67 Air.seek :pressurized @23:10:59.12 14.8psi after pressurize @23:10:59.16 Air.in :compress @23:10:59.26 Air.seek :evac @23:11:03.93 Reached 20.2 of 20.0psi after pumping 5211uL (2.93 strokes) @23:11:03.95 Air.in :slow @23:11:04.08 Air.hold @23:11:04.23 Plunge.seek :puckBottom @23:11:05.14 delay 3 seconds @23:11:08.16 Handoff.select :waste @23:11:08.69 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:11:24.33 delay 1:00 @23:12:24.36 Handoff.select :blocked @23:12:24.90 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @23:12:47.50 Handoff.select :waste @23:12:48.01 0.2psi at evacEnd @23:12:48.04 Plunge.seek :pTop @23:12:48.97 delay 5 seconds @23:12:54.00 Air.in :glide @23:12:54.09 Air.seek :fullVent @23:12:56.93 delay 2 seconds @23:12:58.96 Plunge.seek :pBottom @23:12:59.86 Seawater evacuated, Pressure above/below filter released. @23:12:59.89 Plunge.seek :locked @23:13:01.24 Handoff.select :waste @23:13:01.41 Lysis1.in :slow @23:13:01.50 Lysis1.seek 950uL @23:13:15.61 delay 5 seconds @23:13:20.64 Handoff.select :blocked @23:13:21.16 Plunge.seek :puckTop @23:13:22.26 Lysis1.seek 350uL @23:13:50.34 delay 3 seconds @23:13:53.46 Air.in mix with 2.79934 parts Lysis1 @23:13:54.21 Air.seek :evac @23:13:54.37 Lysis1.seek 190uL @23:14:02.04 Air.in :fast @23:14:02.13 Air.seek :full @23:14:08.67 Lysis1.seek :empty @23:14:17.80 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @10.4psi @23:14:17.83 Plunge.seek :puckBottom @23:14:18.89 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @8.8psi @23:14:18.93 Polling Can every 2:00 @23:14:19.51 PuckHeater.configure :default #ambient 28.9C @23:14:19.60 PuckHeater.seek 81.3C @23:15:32.40 PuckHeater.hold 2:00,81.3C @23:17:32.37 Polling Can every 2:00 @23:17:32.92 PuckHeater.seek 80.2C @23:17:33.14 PuckHeater.hold 2:00,80.2C @23:19:33.68 Polling Can every 2:00 @23:19:34.24 PuckHeater.configure :default #ambient 28.8C @23:19:34.37 PuckHeater.hold 8:00,79.2C @23:27:33.75 Lysate cooked @7.8psi @23:27:33.78 Plunge.seek :puckTop @23:27:34.70 Air syringe pumping up to 5 strokes to reach 40psi @23:27:34.72 Air stroke #1 of 5 @23:27:34.86 Air.in :pressurize @23:27:34.94 Air.seek :pressurized @23:27:41.36 7.9psi after pressurize @23:27:41.39 Air.in :compress @23:27:41.49 Air.seek :evac @23:27:47.17 Reached 41.0 of 40.0psi after pumping 1718uL (0.97 strokes) @23:27:47.20 Air.in :slow @23:27:47.30 Air.hold @23:27:47.40 delay 2 seconds @23:27:49.43 Air.in :fast @23:27:49.57 Air.seek :full @23:27:55.90 Plunge.seek :puckBottom @23:27:56.89 Lysis1.seek 138uL @23:28:03.48 delay 2 seconds @23:28:05.50 Plunge.seek :puckTop @23:28:06.86 Lysis1.seek :empty @23:28:13.46 Plunge.seek :puckBottom @23:28:14.63 Lysis1.in :extract @23:28:14.76 Lysis1.seek 675uL,nil @23:28:18.49 Air.in :pressurize @23:28:18.58 Air.seek :pressurized @23:28:18.89 Syringe::LysatePlungerStuck in PROCESSING -- at < 419uL during Lysis Syringe #1 (LyS1) move @23:28:18.93 Lysis1.in :default @23:28:19.04 Lysis1.seek 389uL @23:28:25.00 13.8psi after pressurize @23:28:25.03 Air.in :compressLysate @23:28:25.13 Air.seek :evac @23:28:29.61 Reached 15.5 of 15.5psi after pumping 1468uL (0.83 strokes) @23:28:29.63 Air.in :slow @23:28:29.74 Air.hold @23:28:29.90 Lysis1.in :extract @23:28:30.06 Lysis1.seek 675uL,nil @23:28:32.06 Air.in :compressLysate @23:28:32.16 Syringe::LysatePlungerStuck in PROCESSING -- at < 461uL during Lysis Syringe #1 (LyS1) move @23:28:32.22 Air.seek :evac @23:28:32.32 Lysis1.in :default @23:28:32.52 Lysis1.seek 431uL @23:28:33.93 Reached 16.2 of 15.5psi after pumping 1487uL (0.84 strokes) @23:28:33.98 Air.in :slow @23:28:34.06 Air.hold @23:28:34.46 Lysis1.in :extract @23:28:34.62 Lysis1.seek 675uL,nil @23:28:36.28 Air.in :compressLysate @23:28:36.43 Air.seek :evac @23:28:36.81 Syringe::LysatePlungerStuck in PROCESSING -- at < 596uL during Lysis Syringe #1 (LyS1) move @23:28:36.89 Lysis1.in :default @23:28:36.98 Lysis1.seek 566uL @23:28:40.45 Reached 15.7 of 15.5psi after pumping 1541uL (0.87 strokes) @23:28:40.48 Air.in :slow @23:28:40.60 Air.hold @23:28:40.76 Lysis1.in :extract @23:28:40.90 Lysis1.seek 675uL,nil @23:28:42.28 Air.in :compressLysate @23:28:42.39 Air.seek :evac @23:28:42.72 Syringe::LysatePlungerStuck in PROCESSING -- at < 642uL during Lysis Syringe #1 (LyS1) move @23:28:42.76 Lysis1.in :default @23:28:42.88 Lysis1.seek 612uL @23:28:43.99 Reached 15.6 of 15.5psi after pumping 1560uL (0.88 strokes) @23:28:44.06 Air.in :slow @23:28:44.21 Air.hold @23:28:44.25 Lysis1.in :extract @23:28:45.17 Lysis1.seek 675uL,nil @23:28:46.78 Pulled 675uL lysate @14.4psi @23:28:47.01 Lysis1.seek 800uL,nil @23:28:47.28 Air.in :compressLysate @23:28:47.38 Air.seek :evac @23:28:56.47 Air.in :slow @23:28:56.54 Air.hold @23:28:56.90 Air.in :glide @23:28:56.99 Air.seek :fullVent @23:28:59.56 Handoff.select :waste @23:29:00.08 Recovered 800uL lysate @15.6psi @23:29:00.10 delay 3 seconds @23:29:03.73 After 3 delay Recovered 800uL lysate @0.4psi @23:29:03.89 Plunge.seek :locked @23:29:05.24 Lysis2.in :DAmix @23:29:05.39 Lysis1.in :DAmix @23:29:06.02 Lysis2.seek 700uL @23:29:06.09 Lysis1.seek 550uL @23:29:41.20 delay 5 seconds @23:29:46.22 Handoff.select SPR @23:29:46.80 SPR.command "start 2 14" @23:29:48.16 SPR stabilized @23:29:48.40 delay 20 seconds @23:30:08.42 SPR.command "start 2 0" @23:30:11.52 Waiting up to 11:00 for SPR to enter state 1 @23:32:09.61 SPR.command "hold_on" @23:32:11.53 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:32:12.31 Lysis2.seek 400uL @23:32:12.44 Lysis1.seek 250uL @23:32:54.40 delay 15 seconds @23:33:09.42 SPR.command "hold_off" @23:33:13.13 Waiting up to 1:00:00 for SPR to enter state 7 @23:49:28.18 SPRsummary:1.43RIU,65.1RIU,62.8RIU,99.9RIU,134ng/L,139ng/L,154ng/L after 3489s @00:03:45.07EDT01-Aug-26 SPR analysis completed @00:03:51.37 Ended log spr26jul3123h.spr.bz2 @00:03:51.39 SPR.command "stats_off" @00:03:52.21 Selecting Cartridge 45 @00:03:52.46 Gate.power :heat,:OFF @00:03:52.48 Gate.power :cartridge,:OFF @00:03:52.61 PinPort.in :unlocking @00:03:52.70 PinPort.seek :clear @00:03:53.55 Carousel.in :default @00:03:53.71 Carousel.home.seek 30 @00:03:54.10 Archive.in :unlocking @00:03:54.25 Archive.seek :clear @00:03:54.42 Air.in :unlocking @00:03:55.06 Air.seek :clear @00:03:55.23 Lysis2.in :unlocking @00:03:55.30 Lysis2.seek :clear @00:03:59.84 Lysis1.in :unlocking @00:04:00.01 Lysis1.seek :clear @00:04:00.11 Plunge.in :unlocking @00:04:00.24 Plunge.seek :clear @00:04:04.28 Carousel.seek 45 @00:04:07.77 Carousel.reconfigure :align @00:04:07.93 Lysis1.in :fast @00:04:08.01 Lysis1.seek :locked @00:04:10.67 Plunge.in :default @00:04:10.75 Plunge.seek :locked @00:04:10.93 Air.in :fast @00:04:11.00 Air.seek :locked @00:04:12.11 Carousel.reconfigure :locked @00:04:12.37 Powering cartridge bus @00:04:15.04 Gate.setIR 1750,5000,0,6 @00:04:15.17 Gate.setIR 0,5000,0,6 @00:04:15.30 Gate.power :cartridge,:OFF @00:04:15.35 Mounted archive Cartridge @00:04:15.69 Preparing to filter archiveHiBiomass_bac cartridge in slot 45 @00:04:15.97 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @00:04:22.60 Intake Head Pressure tare is 0% of full scale at 29C (drifted 6%) @00:04:22.62 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @00:04:22.69 PhaseSeparator.in :filling @00:04:22.76 PhaseSeparator.hold @00:04:22.86 Gate.power :sampler,:ON @00:04:29.57 Head pressure reached 3.95psi in 7 seconds @00:04:29.68 Priming sample loop w/200ml, bypass w/10ml @00:04:29.70 SamplePump.setPosition! 0ml @00:04:29.79 Bypass.open @00:04:32.13 Intake.open @00:04:33.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @00:04:36.81 Vacuum tare is 54% of full scale at 29C (drifted 2%) @00:04:36.99 Abs.in :default @00:04:37.07 Abs.hold @00:04:37.17 PhaseSeparator.in :full @00:04:37.25 PhaseSeparator.hold @00:04:37.52 Waiting for sampler to finish priming... @00:04:37.54 delayUntil "00:05:22.88" @00:05:22.96 SamplePump.in :start @00:05:23.07 SamplePump.seek 10ml,1:10 @00:05:23.65 SamplePump.configure :prime @00:05:36.62 Abs.stop @00:05:36.71 PhaseSeparator.stop @00:05:36.78 SamplePump.setPosition! 0ml @00:05:36.86 Exhaust.open @00:05:39.12 Bypass.close @00:05:41.51 Abs.in :default @00:05:41.60 Abs.hold @00:05:41.71 PhaseSeparator.hold @00:05:42.08 SamplePump.in :start @00:05:42.19 SamplePump.seek 200ml,13:50 @00:05:42.77 SamplePump.configure :prime @00:10:04.82 Abs.stop @00:10:04.88 PhaseSeparator.stop @00:10:05.01 Bypass.open @00:10:06.57 Intake.close @00:10:08.14 Toroid.in :hold @00:10:08.23 Toroid.seek :crack @00:10:10.73 Toroid.seek :cartridge @00:10:12.89 Toroid.in :default @00:10:12.98 Toroid.seek :clear @00:10:16.98 Pressurizing cartridge to 26psi above 15.6psia @00:10:17.01 SamplePump.setPosition! 0ml @00:10:17.09 Intake.open @00:10:18.58 Bypass.close @00:10:20.12 Exhaust.close @00:10:21.82 Abs.in :default @00:10:21.90 Abs.hold @00:10:22.48 PhaseSeparator.hold @00:10:22.87 SamplePump.in :charge @00:10:22.97 SamplePump.seek 2.3ml,40 seconds @00:10:37.16 Abs.stop @00:10:37.25 PhaseSeparator.stop @00:10:37.33 Intake.close @00:10:38.90 Pressurized cartridge with 0.836ml to 29.5psi @00:10:38.92 Isolated @36.5psia (20.8psi) @00:10:38.97 Verifying cartridge holds at least 0.65..0.85 of 36.5psia @00:10:39.03 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @00:10:43.65 Cartridge pressure fell 1% to 36.3psia @00:10:43.78 Sampling 1000.0ml @00:10:43.80 SamplePump.setPosition! 0ml @00:10:43.89 Exhaust.open @00:10:45.44 Intake.open @00:10:47.07 Abs.in :default @00:10:47.16 Abs.hold @00:10:47.27 PhaseSeparator.hold @00:10:47.66 SamplePump.in :start @00:10:47.76 SamplePump.seek 1000ml,1:07:10 @00:10:48.35 SamplePump.configure :sample @00:10:50.76 Cartridge 45 filtering 2/1000ml at 0.76ml/s with 17.0psi vacuum @00:11:50.83 Cartridge 45 filtering 47/1000ml at 0.75ml/s with 21.8psi vacuum @00:12:50.81 Cartridge 45 filtering 91/1000ml at 0.74ml/s with 18.9psi vacuum @00:13:50.82 Cartridge 45 filtering 135/1000ml at 0.72ml/s with 20.9psi vacuum 4.0m deep (20.6psia) @00:14:50.80 Cartridge 45 filtering 177/1000ml at 0.71ml/s with 17.2psi vacuum @00:15:50.82 Cartridge 45 filtering 219/1000ml at 0.69ml/s with 16.1psi vacuum 4.9m deep (21.9psia) @00:16:50.81 Cartridge 45 filtering 258/1000ml at 0.66ml/s with 23.7psi vacuum 3.9m deep (20.5psia) @00:17:50.81 Cartridge 45 filtering 294/1000ml at 0.60ml/s with 40.8psi vacuum @00:18:50.81 Cartridge 45 filtering 327/1000ml at 0.55ml/s with 22.2psi vacuum @00:19:50.81 Cartridge 45 filtering 355/1000ml at 0.47ml/s with 20.6psi vacuum @00:20:50.81 Cartridge 45 filtering 379/1000ml at 0.41ml/s with 33.9psi vacuum @00:21:51.28 Cartridge 45 filtering 399/1000ml at 0.34ml/s with 16.9psi vacuum 5.6m deep (23.0psia) @00:22:50.81 Cartridge 45 filtering 416/1000ml at 0.28ml/s with 39.8psi vacuum @00:23:50.82 Cartridge 45 filtering 431/1000ml at 0.25ml/s with 19.9psi vacuum 3.9m deep (20.5psia) @00:23:50.97 SamplePump.stop @00:23:51.06 Intake.close @00:23:51.16 Exhaust.close @00:23:51.25 Handoff.select :close @00:23:51.58 Toroid.in :hold @00:23:51.67 Toroid.seek :bypass @00:23:55.30 Toroid.in :default @00:23:55.40 Toroid.seek :clear @00:23:59.00 Cartridge::Sampler::Stuffed in FILTERING -- Cartridge clogged @00:23:59.03 Abs.stop @00:23:59.13 PhaseSeparator.stop @00:23:59.31 Sampled 431.2ml @00:23:59.98 Plunge.seek :pTop @00:24:00.90 Sample loop closed. Ready to Deliver Reagents @00:24:00.93 Air.in :slow @00:24:01.03 Air.seek :sealed @00:24:02.47 Gate.power :sampler,:OFF @00:24:13.26 Air.seek :arEvac @00:25:27.90 delay 1:00 @00:26:27.95 Air.seek :clear @00:28:05.18 RNAlater soak for 10:00 @00:38:05.22 Air.seek :tVent @00:38:15.97 delay 1 second @00:38:17.00 Air.in :halfSpeed @00:38:17.08 Air.seek :arEmpty @00:38:49.79 delay 10 seconds @00:38:59.83 delay 1:00 @00:39:59.87 Plunge.seek :pTop @00:40:00.03 Sample preserved and evacuated @00:40:00.43 Carousel.in :default @00:40:00.58 Carousel.home.seek 45 @00:40:00.94 Plunge.seek :locked @00:40:02.02 Air.in :unlocking @00:40:02.17 Air.seek :clear @00:40:02.35 Lysis1.in :unlocking @00:40:02.49 Lysis1.seek :clear @00:40:07.03 Gate.power :motor,:OFF @00:40:07.05 Gate.power :analytic1,:OFF @00:40:07.55 Gate.power :core,:OFF @00:40:07.65 Gate.power :main,:OFF @00:40:07.69 Safely stopped and ready to power off @09:31:09.79 -> ESP.reready! @09:31:09.84 Gate.power :sampler,:OFF,limit to 3500mA @09:31:09.88 Gate.power :skimmer,:OFF,limit to 3500mA @09:31:09.92 Gate.power :main,:ON,limit to 5000mA @09:31:09.95 Gate.power :analytic1,:ON,limit to 4000mA @09:31:10.06 Powering core bus @09:31:12.43 SPR Module 1, FW Rev. B, FPGA Rev. C @09:31:12.86 Plunge.configure :default @09:31:12.97 SamplePump.configure :default @09:31:13.09 Toroid.configure :default @09:31:13.19 Carousel.configure :default @09:31:13.30 Abs.configure :default @09:31:13.41 PhaseSeparator.configure :full @09:31:13.51 Archive.configure :default @09:31:13.60 Air.configure :slow @09:31:13.69 Lysis1.configure :default @09:31:13.77 Lysis2.configure :default @09:31:13.88 PinPort.configure :default @09:31:13.95 All elf controllers running firmware version 4.94 @09:31:13.98 Gate.power :motor,:ON,limit to 5000mA @09:31:14.07 Plunge.home.jog 0 @09:31:14.16 Toroid.home.jog 0 @09:31:14.27 Carousel.home.jog 0 @09:31:14.57 Archive.home.jog 0 @09:31:14.71 Air.in :default @09:31:14.84 Lysis1.home.jog 0 @09:31:14.93 Lysis2.home.jog 0 @09:31:14.98 PinPort.home.jog 0 @09:31:15.33 Air.home.jog 0 @09:31:15.92 Plunge.in :homing @09:31:16.76 Plunge.jog 1477 @09:31:17.01 PinPort.in :homing @09:31:17.05 Lysis2.in :homing @09:31:17.10 Air.in :homing @09:31:17.14 Toroid.in :homing @09:31:17.19 Lysis1.in :homing @09:31:17.22 Archive.in :homing @09:31:17.52 PinPort.jog 1238 @09:31:17.58 Lysis2.jog 1615 @09:31:17.64 Air.jog 1428 @09:31:17.69 Toroid.jog 1356 @09:31:17.76 Archive.jog 1611 @09:31:17.85 Lysis1.jog 303 @09:31:18.27 Plunge.setRawPosition! 50 @09:31:18.38 Plunge.seek :home @09:31:18.70 Lysis1.setRawPosition! 50 @09:31:18.73 Plunge.in :default @09:31:18.88 Lysis1.seek :home @09:31:19.13 Air.setRawPosition! 0 @09:31:19.22 Air.seek :home @09:31:19.37 Lysis2.setRawPosition! 50 @09:31:19.42 Lysis1.in :default @09:31:19.47 Archive.setRawPosition! 50 @09:31:19.51 Air.in :slow @09:31:19.72 Lysis2.seek :home @09:31:19.82 Archive.seek :home @09:31:19.98 PinPort.setRawPosition! 3150 @09:31:20.05 PinPort.seek :home @09:31:20.21 Archive.in :default @09:31:20.24 Lysis2.in :default @09:31:22.58 Toroid.setRawPosition! 0 @09:31:22.67 Toroid.seek :home @09:31:22.77 Toroid.in :default @09:31:24.46 PinPort.in :default @09:31:24.82 Toroid.seek :clear @09:31:25.03 PinPort.in :unlocking @09:31:25.11 PinPort.seek :clear @09:31:28.53 Air.seek :clear @09:31:28.66 Lysis1.seek :clear @09:31:29.41 Lysis2.seek :clear @09:31:30.24 Archive.seek :clear @09:31:31.06 Carousel.in :default @09:31:31.21 Carousel.home.seek 45 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 45 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @0.00psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @09:31:33.67 -> SC.coast @09:31:33.71 Carousel.coast Storage Carousel (SC) at 45 @09:35:43.46 -> Head.psi 0.0226961267975081 @09:38:26.31 -> ESP.reready! @09:38:26.40 Plunge.in :default @09:38:26.45 Toroid.in :default @09:38:27.08 Archive.in :default @09:38:27.19 Carousel.in :default @09:38:27.31 Air.in :default @09:38:27.36 Plunge.home.jog 0 @09:38:27.50 Lysis1.in :default @09:38:27.58 Lysis2.in :default @09:38:27.63 PinPort.in :default @09:38:27.75 Toroid.home.jog 0 @09:38:27.85 Archive.home.jog 0 @09:38:28.02 Carousel.home.jog 0 @09:38:28.10 Air.home.jog 0 @09:38:28.26 Lysis1.home.jog 0 @09:38:28.37 Lysis2.home.jog 0 @09:38:28.46 PinPort.home.jog 0 @09:38:29.33 Plunge.in :homing @09:38:29.64 Plunge.jog 431 @09:38:29.72 PinPort.in :homing @09:38:29.77 Lysis1.in :homing @09:38:29.81 Archive.in :homing @09:38:29.86 Air.in :homing @09:38:29.90 Lysis2.in :homing @09:38:29.94 Toroid.in :homing @09:38:30.26 PinPort.jog 1227 @09:38:30.39 Lysis1.jog 1593 @09:38:30.45 Archive.jog 1612 @09:38:30.52 Air.jog 1426 @09:38:30.60 Lysis2.jog 1615 @09:38:30.68 Toroid.jog 1356 @09:38:31.07 Plunge.setRawPosition! 50 @09:38:31.14 Plunge.seek :home @09:38:31.45 Plunge.in :default @09:38:32.00 Lysis1.setRawPosition! 50 @09:38:32.11 Air.setRawPosition! 0 @09:38:32.16 Lysis1.seek :home @09:38:32.36 Air.seek :home @09:38:32.52 PinPort.setRawPosition! 3150 @09:38:32.57 Lysis2.setRawPosition! 50 @09:38:32.60 Archive.setRawPosition! 50 @09:38:32.64 Air.in :slow @09:38:32.84 PinPort.seek :home @09:38:32.88 Lysis2.seek :home @09:38:32.93 Archive.seek :home @09:38:33.24 Lysis1.in :default @09:38:33.35 Lysis2.in :default @09:38:33.48 Archive.in :default @09:38:35.38 Toroid.setRawPosition! 0 @09:38:35.45 Toroid.seek :home @09:38:35.57 Toroid.in :default @09:38:37.41 PinPort.in :default @09:38:37.78 Toroid.seek :clear @09:38:37.97 PinPort.in :unlocking @09:38:38.05 PinPort.seek :clear @09:38:41.48 Air.seek :clear @09:38:41.61 Lysis1.seek :clear @09:38:42.37 Lysis2.seek :clear @09:38:43.19 Archive.seek :clear @09:38:44.04 Carousel.in :default @09:38:44.20 Carousel.home.seek 23 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 23 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @0.02psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @09:38:53.46 -> delay 5;to 30 @09:38:53.49 delay 5 seconds @09:38:58.81 Carousel.seek 30 @09:39:00.62 Carousel.reconfigure :align @09:39:00.77 Lysis1.in :fast @09:39:00.86 Lysis1.seek :locked @09:39:03.50 Plunge.seek :locked @09:39:03.70 Air.in :fast @09:39:03.78 Air.seek :locked @09:39:04.88 Carousel.reconfigure :locked @09:39:05.05 Powering cartridge bus @09:39:07.72 Gate.setIR 1750,5000,0,6 @09:39:07.85 Gate.setIR 0,5000,0,6 @09:39:07.93 PuckHeater.configure :default #ambient 28.8C @09:39:08.02 Gate.power :heat,:ON,limit to 5000mA @09:39:08.21 Mounted lysis Cartridge board#J59913 @09:39:08.26 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @09:39:22.27 -> to 40 @09:39:22.50 Gate.power :heat,:OFF @09:39:22.52 Gate.power :cartridge,:OFF @09:39:22.65 Carousel.in :default @09:39:22.79 Carousel.home.seek 30 @09:39:23.25 Air.in :unlocking @09:39:23.37 Air.seek :clear @09:39:24.48 Lysis1.in :unlocking @09:39:24.62 Lysis1.seek :clear @09:39:24.75 Plunge.in :unlocking @09:39:24.84 Plunge.seek :clear @09:39:25.98 Carousel.seek 40 @09:39:28.44 Carousel.reconfigure :align @09:39:28.60 Lysis1.in :fast @09:39:28.68 Lysis1.seek :locked @09:39:31.82 Plunge.in :default @09:39:31.92 Plunge.seek :locked @09:39:32.11 Air.in :fast @09:39:32.20 Air.seek :locked @09:39:33.29 Carousel.reconfigure :locked @09:39:33.48 Powering cartridge bus @09:39:36.18 Gate.setIR 1750,5000,0,6 @09:39:36.34 Gate.setIR 0,5000,0,6 @09:39:36.43 PuckHeater.configure :default #ambient 28.8C @09:39:36.52 Gate.power :heat,:ON,limit to 5000mA @09:39:36.72 Mounted lysis Cartridge board#J59986 @09:39:36.76 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @09:39:59.22 -> SC.coast @09:39:59.25 Carousel.coast Storage Carousel (SC) at 40 @09:40:04.43 -> Cart.unlock @09:40:04.49 Gate.power :heat,:OFF @09:40:04.50 Gate.power :cartridge,:OFF @09:40:04.62 Carousel.in :default @09:40:04.77 Carousel.home.seek 40 @09:40:05.23 Air.in :unlocking @09:40:05.37 Air.seek :clear @09:40:06.46 Lysis1.in :unlocking @09:40:06.60 Lysis1.seek :clear @09:40:06.74 Plunge.in :unlocking @09:40:06.83 Plunge.seek :clear @09:40:10.10 -> SC.coastt @09:40:10.16 NoMethodError in preston -- undefined method `coastt' for Carousel:Slide @09:40:12.75 -> SC.coast @09:40:12.77 Carousel.coast Storage Carousel (SC) at 40 @09:46:26.02 -> Power.sampler :ON @09:46:26.05 Gate.power :sampler,:ON true @09:47:45.14 -> Power.sampler :OFF @09:47:45.17 Gate.power :sampler,:OFF false @09:50:12.34 -> Power.sampler :ON @09:50:12.38 Gate.power :sampler,:ON true @09:50:24.22 -> 3.times{Sampler.refill} @09:50:24.25 Bypass.open @09:50:25.85 Intake.open @09:50:27.35 SamplePump.setPosition! 0ml @09:50:27.49 SamplePump.seek 30ml @09:51:07.46 Bypass.close @09:51:09.00 Exhaust.open @09:51:10.60 SamplePump.seek 55ml @09:51:44.02 delay 1 second @09:51:45.05 Intake.close @09:51:45.15 Exhaust.close @09:51:46.75 Bypass.open @09:51:48.34 Intake.open @09:51:49.83 SamplePump.setPosition! 0ml @09:51:49.97 SamplePump.seek 30ml @09:52:29.93 Bypass.close @09:52:31.48 Exhaust.open @09:52:33.10 SamplePump.seek 55ml @09:53:06.51 delay 1 second @09:53:07.63 Intake.close @09:53:07.68 Exhaust.close @09:53:09.34 Bypass.open @09:53:10.88 Intake.open @09:53:12.38 SamplePump.setPosition! 0ml @09:53:12.52 SamplePump.seek 30ml @09:53:52.49 Bypass.close @09:53:54.01 Exhaust.open @09:53:55.59 SamplePump.seek 55ml @09:54:29.01 delay 1 second @09:54:30.04 Intake.close @09:54:30.12 Exhaust.close 3 @09:54:39.95 -> BACsampler.prime @09:54:40.07 Priming sample loop w/200ml, bypass w/10ml @09:54:40.09 SamplePump.setPosition! 0ml @09:54:40.17 Bypass.open @09:54:42.47 Intake.open @09:54:44.00 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:54:46.63 Vacuum tare is 53% of full scale at 27C (drifted 1%) @09:54:46.80 Abs.in :default @09:54:46.88 Abs.hold @09:54:46.98 PhaseSeparator.hold @09:54:47.27 SamplePump.in :start @09:54:47.36 SamplePump.seek 10ml,1:10 @09:54:47.96 SamplePump.configure :prime @09:55:00.92 Abs.stop @09:55:00.99 PhaseSeparator.stop @09:55:01.06 SamplePump.setPosition! 0ml @09:55:01.14 Exhaust.open @09:55:03.38 Bypass.close @09:55:05.78 Abs.in :default @09:55:05.86 Abs.hold @09:55:05.97 PhaseSeparator.hold @09:55:06.36 SamplePump.in :start @09:55:06.44 SamplePump.seek 200ml,13:50 @09:55:07.05 SamplePump.configure :prime @09:56:13.24 SamplePump.stop @09:56:13.40 Cartridge::Sampler::LostHeadPressure in preston -- Head Pressure Fell to 0.73psi @09:56:13.45 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @09:56:13.50 PhaseSeparator.in :filling @09:56:13.58 PhaseSeparator.hold @09:56:14.19 Gate.power :sampler,:OFF @09:56:14.57 Gate.power :sampler,:ON @09:56:23.96 PhaseSeparator.stop @09:56:24.07 Abs.stop @09:56:24.16 UserAbort in preston -- User Abort!! @09:56:27.55 -> BACsampler.prime @09:56:27.66 Priming sample loop w/200ml, bypass w/10ml @09:56:27.68 SamplePump.setPosition! 0ml @09:56:27.77 Exhaust.close @09:56:29.32 Bypass.open @09:56:29.40 UserAbort in preston -- deferring User Abort!! @09:56:29.46 until Bypass (PB) finishes opening @09:56:51.32 UserAbort in preston -- Bypass (PB) could not finish opening because User Abort!! @09:56:51.40 -> Power.sampler :OFF @09:56:51.44 Gate.power :sampler,:OFF false @09:56:54.35 -> Power.sampler :OFF @09:56:54.37 Gate.power :sampler,:OFF false @10:05:12.99 -> Power.sampler :ON @10:05:13.01 Gate.power :sampler,:ON true @10:05:21.22 -> BACsampler.prime @10:05:21.34 Priming sample loop w/200ml, bypass w/10ml @10:05:21.37 SamplePump.setPosition! 0ml @10:05:21.45 Bypass.open @10:05:23.87 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:05:26.50 Vacuum tare is 53% of full scale at 27C (drifted 1%) @10:05:26.67 Abs.in :default @10:05:26.75 Abs.hold @10:05:26.85 PhaseSeparator.in :full @10:05:26.93 PhaseSeparator.hold @10:05:27.18 Waiting for sampler to finish priming... @10:05:27.20 delayUntil "10:06:13.04" @10:05:31.09 Intake.close @10:05:31.19 Bypass.close @10:05:32.96 Abs.stop @10:05:33.04 PhaseSeparator.stop @10:05:33.13 UserAbort in preston -- User Abort!! @10:05:44.67 -> 2.times{Sampler.refill} @10:05:44.69 Bypass.open @10:05:46.28 Intake.open @10:05:47.76 SamplePump.setPosition! 0ml @10:05:47.90 SamplePump.in :default @10:05:47.98 SamplePump.seek 30ml @10:05:52.28 SamplePump.hold @10:05:52.53 delay 1 second @10:05:53.57 Intake.close @10:05:53.62 Bypass.close @10:05:55.34 UserAbort in preston -- Sample Pump (SP) seek to 30ml Aborted! @10:06:07.55 -> Power.sampler :OFF @10:06:07.58 Gate.power :sampler,:OFF false @10:06:27.33 -> Sampler.refill @10:06:27.35 Bypass.open @10:06:28.93 Intake.open @10:06:30.37 SamplePump.setPosition! 0ml @10:06:30.52 SamplePump.seek 30ml @10:07:10.48 Bypass.close @10:07:12.01 Exhaust.open @10:07:13.59 SamplePump.seek 55ml @10:07:46.99 delay 1 second @10:07:48.03 Intake.close @10:07:48.08 Exhaust.close Sample Pump (SP) at 55ml @10:09:35.26 -> Sampler.refill @10:09:35.28 Bypass.open @10:09:36.85 Intake.open @10:09:38.35 SamplePump.setPosition! 0ml @10:09:38.49 SamplePump.seek 30ml @10:10:18.45 Bypass.close @10:10:20.02 Exhaust.open @10:10:21.67 SamplePump.seek 55ml @10:10:50.81 SamplePump.hold @10:10:51.04 delay 1 second @10:10:52.08 Intake.close @10:10:52.19 Exhaust.close @10:10:53.91 UserAbort in preston -- Sample Pump (SP) seek to 55ml Aborted! @10:14:14.35 -> Power.sampler :ON @10:14:14.38 Gate.power :sampler,:ON true @10:14:20.02 -> Power.sampler :OFF @10:14:20.05 Gate.power :sampler,:OFF false @10:14:30.58 -> Sampler.refill @10:14:30.61 Bypass.open @10:14:32.20 Intake.open @10:14:33.67 SamplePump.setPosition! 0ml @10:14:33.81 SamplePump.seek 30ml @10:15:13.78 Bypass.close @10:15:15.36 Exhaust.open @10:15:16.96 SamplePump.seek 55ml @10:15:50.36 delay 1 second @10:15:51.39 Intake.close @10:15:51.47 Exhaust.close Sample Pump (SP) at 55ml @10:24:20.82 -> Head.psi 0.0148085461046774 @10:27:20.83 -> 2.times{BACsampler.prime 200,100} @10:27:20.94 Priming sample loop w/200ml, bypass w/100ml @10:27:20.96 SamplePump.setPosition! 0ml @10:27:21.05 Bypass.open @10:27:22.84 UserAbort in preston -- User Abort!! @10:27:35.06 -> Power.sampler :ON @10:27:35.09 Gate.power :sampler,:ON true @10:27:39.15 -> 2.times{BACsampler.prime 200,100} @10:27:39.26 Priming sample loop w/200ml, bypass w/100ml @10:27:39.28 SamplePump.setPosition! 0ml @10:27:40.15 Intake.open @10:27:41.70 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:27:44.37 Vacuum tare is 53% of full scale at 27C (drifted 2%) @10:27:44.55 Abs.in :default @10:27:44.62 Abs.hold @10:27:44.73 PhaseSeparator.hold @10:27:44.99 Waiting for sampler to finish priming... @10:27:45.00 delayUntil "10:28:35.12" @10:28:21.81 SamplePump.stop @10:28:21.98 Cartridge::Sampler::LostHeadPressure in preston -- Head Pressure Fell to 0.88psi @10:28:22.01 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @10:28:22.66 PhaseSeparator.in :filling @10:28:22.78 PhaseSeparator.hold @10:28:22.89 Gate.power :sampler,:OFF @10:28:23.28 Gate.power :sampler,:ON @10:28:29.93 Head pressure reached 4.42psi in 8 seconds @10:28:29.96 Abs.hold @10:28:30.08 PhaseSeparator.in :full @10:28:30.16 PhaseSeparator.hold @10:28:30.44 Waiting for sampler to finish priming... @10:28:30.46 delayUntil "10:29:23.30" @10:29:23.36 SamplePump.in :start @10:29:23.46 SamplePump.seek 100ml,7:10 @10:29:24.05 SamplePump.configure :prime @10:31:35.02 Abs.stop @10:31:35.10 PhaseSeparator.stop @10:31:35.18 SamplePump.setPosition! 0ml @10:31:35.26 Exhaust.open @10:31:37.49 Bypass.close @10:31:39.89 Abs.in :default @10:31:39.98 Abs.hold @10:31:40.09 PhaseSeparator.hold @10:31:40.49 SamplePump.in :start @10:31:40.59 SamplePump.seek 200ml,13:50 @10:31:41.18 SamplePump.configure :prime @10:36:03.32 Abs.stop @10:36:03.40 PhaseSeparator.stop @10:36:03.54 Priming sample loop w/200ml, bypass w/100ml @10:36:03.56 SamplePump.setPosition! 0ml @10:36:03.66 Exhaust.close @10:36:05.18 Bypass.open @10:36:07.65 Abs.in :default @10:36:07.73 Abs.hold @10:36:07.83 PhaseSeparator.hold @10:36:08.12 SamplePump.in :start @10:36:08.22 SamplePump.seek 100ml,7:10 @10:36:08.82 SamplePump.configure :prime @10:38:19.77 Abs.stop @10:38:19.84 PhaseSeparator.stop @10:38:19.91 SamplePump.setPosition! 0ml @10:38:19.99 Exhaust.open @10:38:22.24 Bypass.close @10:38:24.68 Abs.in :default @10:38:24.76 Abs.hold @10:38:24.87 PhaseSeparator.hold @10:38:25.80 SamplePump.in :start @10:38:25.91 SamplePump.seek 200ml,13:50 @10:38:26.49 SamplePump.configure :prime @10:42:48.51 Abs.stop @10:42:48.63 PhaseSeparator.stop 2 @10:43:26.03 -> Power.sampler :OFF @10:43:26.06 Gate.power :sampler,:OFF false @10:43:55.02 -> Power.sampler :ON @10:43:55.05 Gate.power :sampler,:ON true @10:44:40.82 -> Power.sampler :OFF @10:44:40.85 Gate.power :sampler,:OFF false @10:48:08.51 -> SC.coast @10:48:08.53 Carousel.coast Storage Carousel (SC) at 40 @11:06:44.75 -> BACsampler.prime @11:06:44.86 Priming sample loop w/200ml, bypass w/10ml @11:06:44.88 SamplePump.setPosition! 0ml @11:06:44.98 Exhaust.close @11:06:46.54 Bypass.open @11:06:49.05 Abs.in :default @11:06:49.13 Abs.hold @11:06:49.23 PhaseSeparator.hold @11:06:49.51 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:06:49.67 SamplePump.stop @11:06:49.83 Cartridge::Sampler::LostHeadPressure in preston -- Head Pressure Fell to 0.03psi @11:06:49.87 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:06:49.92 PhaseSeparator.in :filling @11:06:50.00 PhaseSeparator.hold @11:06:50.12 Gate.power :sampler,:ON @11:06:57.52 Head pressure reached 1.91psi in 8 seconds @11:06:57.54 Abs.hold @11:06:57.67 PhaseSeparator.in :full @11:06:57.74 PhaseSeparator.hold @11:06:58.01 Waiting for sampler to finish priming... @11:06:58.02 delayUntil "11:07:50.14" @11:07:50.22 SamplePump.in :start @11:07:50.35 SamplePump.seek 10ml,1:10 @11:07:50.91 SamplePump.configure :prime @11:08:03.88 Abs.stop @11:08:03.95 PhaseSeparator.stop @11:08:04.03 SamplePump.setPosition! 0ml @11:08:04.10 Exhaust.open @11:08:06.36 Bypass.close @11:08:08.74 Abs.in :default @11:08:08.82 Abs.hold @11:08:08.93 PhaseSeparator.hold @11:08:09.28 SamplePump.in :start @11:08:09.37 SamplePump.seek 200ml,13:50 @11:08:09.96 SamplePump.configure :prime @11:12:32.60 Abs.stop @11:12:32.76 PhaseSeparator.stop BACsampler @11:14:14.40 -> BACsampler.prime @11:14:14.51 Priming sample loop w/200ml, bypass w/10ml @11:14:14.54 SamplePump.setPosition! 0ml @11:14:14.62 Exhaust.close @11:14:16.18 Bypass.open @11:14:18.69 Abs.in :default @11:14:18.77 Abs.hold @11:14:18.87 PhaseSeparator.hold @11:14:19.15 SamplePump.in :start @11:14:19.26 SamplePump.seek 10ml,1:10 @11:14:19.85 SamplePump.configure :prime @11:14:32.81 Abs.stop @11:14:32.88 PhaseSeparator.stop @11:14:32.95 SamplePump.setPosition! 0ml @11:14:33.03 Exhaust.open @11:14:35.31 Bypass.close @11:14:37.74 Abs.in :default @11:14:37.81 Abs.hold @11:14:37.92 PhaseSeparator.hold @11:14:38.27 SamplePump.in :start @11:14:38.40 SamplePump.seek 200ml,13:50 @11:14:38.96 SamplePump.configure :prime @11:15:46.18 SamplePump.hold @11:15:46.52 Intake.close @11:15:46.63 Exhaust.close @11:15:48.32 Abs.stop @11:15:48.42 PhaseSeparator.stop @11:15:48.53 UserAbort in preston -- Sample Pump (SP) seek to 200ml Aborted! @11:15:52.03 -> sampler :OFF @11:15:52.07 NoMethodError in preston -- undefined method `sampler' for CmdServer::Client:Class @11:15:58.59 -> Power.sampler :OFF @11:15:58.62 Gate.power :sampler,:OFF false @11:16:15.58 -> Power.sampler :ON @11:16:15.60 Gate.power :sampler,:ON true @11:16:28.99 -> Power.sampler :OFF @11:16:29.01 Gate.power :sampler,:OFF false @11:16:46.35 -> to 50 @11:16:46.67 Carousel.seek 50 @11:16:47.10 Carousel.reconfigure :align @11:16:47.25 Lysis1.in :fast @11:16:47.33 Lysis1.seek :locked @11:16:47.39 Slide::Warning in core -- Storage Carousel (SC) reseek at 50 + 5 counts @11:16:50.04 Plunge.in :default @11:16:50.13 Plunge.seek :locked @11:16:50.33 Air.in :fast @11:16:50.43 Air.seek :locked @11:16:51.53 Carousel.reconfigure :locked @11:16:51.72 Powering cartridge bus @11:16:54.42 Gate.power :cartridge,:OFF @11:16:54.48 Mounted archive Cartridge :archiveHiBiomass_bac @11:17:28.59 -> delay 10;TV.cartridge;delay 5;TV.bypass @11:17:28.61 delay 10 seconds @11:17:38.75 Toroid.in :hold @11:17:38.84 Toroid.seek :cartridge @11:17:42.64 Toroid.in :default @11:17:42.72 Toroid.seek :clear @11:17:46.50 delay 5 seconds @11:17:51.62 Toroid.in :hold @11:17:51.71 Toroid.seek :bypass @11:17:55.33 Toroid.in :default @11:17:55.41 Toroid.seek :clear Toroid Valve (TV) clear @11:18:08.83 -> ESP.reready! @11:18:08.89 Plunge.in :default @11:18:08.95 Toroid.in :default @11:18:09.21 Carousel.in :default @11:18:09.27 Archive.in :default @11:18:09.32 Toroid.home.jog 0 @11:18:09.36 Air.in :default @11:18:09.41 Lysis1.in :default @11:18:09.45 Lysis2.in :default @11:18:09.51 Plunge.home.jog 0 @11:18:09.55 PinPort.in :default @11:18:09.84 Carousel.home.jog 0 @11:18:10.12 PinPort.home.jog 0 @11:18:10.23 Lysis1.home.jog 0 @11:18:10.29 Air.home.jog 0 @11:18:10.42 Archive.home.jog 0 @11:18:10.48 Lysis2.home.jog 0 @11:18:11.22 Plunge.in :homing @11:18:11.43 Toroid.in :homing @11:18:11.57 Plunge.jog 1462 @11:18:11.60 Toroid.jog 1360 @11:18:11.96 Air.in :homing @11:18:11.99 PinPort.in :homing @11:18:12.03 Lysis1.in :homing @11:18:12.08 Archive.in :homing @11:18:12.26 PinPort.jog 1225 @11:18:12.33 Air.jog 1233 @11:18:12.43 Lysis1.jog 2265 @11:18:12.49 Archive.jog 1615 @11:18:12.82 Lysis2.in :homing @11:18:12.90 Lysis2.jog 1616 @11:18:13.02 Plunge.setRawPosition! 50 @11:18:13.11 Plunge.seek :home @11:18:13.41 Plunge.in :default @11:18:13.72 Air.setRawPosition! 0 @11:18:13.79 Air.seek :home @11:18:13.95 Archive.setRawPosition! 50 @11:18:13.98 Air.in :slow @11:18:14.12 Archive.seek :home @11:18:14.32 Lysis2.setRawPosition! 50 @11:18:14.37 PinPort.setRawPosition! 3150 @11:18:14.42 Lysis1.setRawPosition! 50 @11:18:15.11 Lysis2.seek :home @11:18:15.26 PinPort.seek :home @11:18:15.34 Lysis1.seek :home @11:18:15.58 Archive.in :default @11:18:15.72 Lysis2.in :default @11:18:15.82 Lysis1.in :default @11:18:16.19 Toroid.setRawPosition! 0 @11:18:16.27 Toroid.seek :home @11:18:16.43 Toroid.in :default @11:18:19.81 PinPort.in :default @11:18:20.19 Toroid.seek :clear @11:18:20.40 PinPort.in :unlocking @11:18:20.49 PinPort.seek :clear @11:18:23.83 Air.seek :clear @11:18:23.95 Lysis1.seek :clear @11:18:24.70 Lysis2.seek :clear @11:18:25.50 Archive.seek :clear @11:18:26.35 Carousel.in :default @11:18:26.50 Carousel.home.seek 50 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 51.57ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 50 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.02psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:18:47.63 -> BACsampler.prime 200,nil @11:18:47.73 Priming sample loop w/200ml @11:18:47.75 SamplePump.setPosition! 0ml @11:18:47.84 Bypass.open @11:18:50.25 Exhaust.open @11:18:52.54 Bypass.close @11:18:52.59 Intake.open @11:18:54.98 Abs.in :default @11:18:55.06 Abs.hold @11:18:55.16 PhaseSeparator.hold @11:18:55.52 SamplePump.stop @11:18:55.67 Cartridge::Sampler::LostHeadPressure in preston -- Head Pressure Fell to 0.02psi @11:18:55.70 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:18:55.76 PhaseSeparator.in :filling @11:18:55.83 PhaseSeparator.hold @11:18:55.95 Gate.power :sampler,:ON @11:19:02.72 Head pressure reached 4.00psi in 7 seconds @11:19:02.74 Abs.hold @11:19:02.88 PhaseSeparator.in :full @11:19:02.95 PhaseSeparator.hold @11:19:03.89 Waiting for sampler to finish priming... @11:19:03.92 delayUntil "11:19:55.97" @11:19:56.05 SamplePump.in :start @11:19:56.14 SamplePump.seek 200ml,13:50 @11:19:56.74 SamplePump.configure :prime @11:23:48.23 SamplePump.hold @11:23:48.56 Intake.close @11:23:48.62 Exhaust.close @11:23:50.36 Abs.stop @11:23:50.47 PhaseSeparator.stop @11:23:50.57 UserAbort in preston -- Sample Pump (SP) seek to 200ml Aborted! @11:23:53.07 -> ampler :OFF @11:23:53.10 NoMethodError in preston -- undefined method `ampler' for CmdServer::Client:Class @11:23:59.07 -> Power.sampler :OFF @11:23:59.09 Gate.power :sampler,:OFF false @11:29:08.73 -> SC.to 22 @11:29:08.78 Carousel.seek 22 Storage Carousel (SC) at 22 @11:30:26.43 -> slots {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 100.0, lysed: 800, spr: spr26jul3119h.spr.bz2}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 100.0, lysed: 800, spr: spr26jul3123h.spr.bz2}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 431.2}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @11:30:53.94 -> slot 22=>[:dry,:microSPR_phyto] {22 => { Type: :microSPR_phyto, State: :dry}} @11:31:39.38 -> MainThread.exit # @11:31:39.58 ThreadError in preston -- killed thread @11:31:39.64 Session Ended @11:31:50.71EDT01-Aug-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @11:31:52.45 >BREAK< ESP3 Gateway v1.44 for core bus @11:31:52.48 >BREAK< ESP3 Gateway v1.44 for cartridge bus @11:31:56.05 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @11:31:56.76 Sea pressure (Abs) tare is 6% of full scale @11:32:00.53 Polling Can every 2:00 @11:32:00.57 Waiting for espclients... @11:32:02.47 Session Begun @11:32:10.58 -> ESP.ready! @11:32:10.77 Gate.power :sampler,:OFF,limit to 3500mA @11:32:10.78 Gate.power :skimmer,:OFF,limit to 3500mA @11:32:10.82 Gate.power :analytic1,:ON,limit to 4000mA @11:32:11.53 Plunge.configure :default @11:32:11.66 SPR Module 1, FW Rev. B, FPGA Rev. C @11:32:11.76 SamplePump.configure :default @11:32:11.92 Toroid.configure :default @11:32:12.01 Toroid.hold @11:32:12.10 Toroid.configure :default @11:32:12.26 Carousel.configure :default @11:32:12.38 Carousel.hold @11:32:12.48 Carousel.configure :default @11:32:12.64 Abs.configure :default @11:32:12.81 PhaseSeparator.configure :default @11:32:12.98 Archive.configure :default @11:32:13.06 Archive.hold @11:32:13.16 Archive.configure :default @11:32:13.35 Air.configure :default @11:32:13.43 Air.hold @11:32:13.53 Air.configure :default @11:32:13.68 Lysis1.configure :default @11:32:13.76 Lysis1.hold @11:32:13.87 Lysis1.configure :default @11:32:14.00 Lysis2.configure :default @11:32:14.08 Lysis2.hold @11:32:14.18 Lysis2.configure :default @11:32:14.38 PinPort.configure :default @11:32:14.46 PinPort.hold @11:32:14.56 PinPort.configure :default @11:32:14.64 All elf controllers running firmware version 4.94 @11:32:15.48 Toroid.seek :clear @11:32:15.70 Air.seek :clear @11:32:15.81 Lysis1.seek :clear @11:32:15.93 Lysis2.seek :clear @11:32:16.46 Archive.seek :clear @11:32:16.59 Carousel.in :default @11:32:16.72 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 176.95ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.59psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:32:20.21 -> slots {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 100, lysed: 800, spr: "spr26jul3119h.spr.bz2"}, 22 => { Type: :microSPR_phyto, State: :dry}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3123h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 431.2}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @11:32:25.38 -> ESP.reready! @11:32:25.42 Plunge.in :default @11:32:25.52 Toroid.in :default @11:32:25.62 Carousel.in :default @11:32:25.66 Plunge.home.jog 0 @11:32:25.77 Archive.in :default @11:32:25.92 Air.in :default @11:32:26.03 Lysis1.in :default @11:32:26.06 Lysis2.in :default @11:32:26.17 PinPort.in :default @11:32:26.22 Toroid.home.jog 0 @11:32:26.31 Carousel.home.jog 0 @11:32:26.49 Archive.home.jog 0 @11:32:26.68 Air.home.jog 0 @11:32:26.80 Lysis1.home.jog 0 @11:32:26.87 Lysis2.home.jog 0 @11:32:27.04 PinPort.home.jog 0 @11:32:27.80 Plunge.in :homing @11:32:28.15 Plunge.jog 419 @11:32:28.24 Lysis2.in :homing @11:32:28.29 Air.in :homing @11:32:28.37 Toroid.in :homing @11:32:28.40 PinPort.in :homing @11:32:28.44 Archive.in :homing @11:32:28.48 Lysis1.in :homing @11:32:28.86 Lysis2.jog 1615 @11:32:28.93 PinPort.jog 1221 @11:32:28.98 Air.jog 1424 @11:32:29.02 Toroid.jog 1364 @11:32:29.06 Lysis1.jog 1595 @11:32:29.12 Archive.jog 1614 @11:32:29.58 Plunge.setRawPosition! 50 @11:32:29.65 Plunge.seek :home @11:32:29.96 Plunge.in :default @11:32:30.47 Lysis2.setRawPosition! 50 @11:32:30.54 Lysis2.seek :home @11:32:30.66 Air.setRawPosition! 0 @11:32:30.74 Air.seek :home @11:32:30.90 Lysis1.setRawPosition! 50 @11:32:30.94 Air.in :default @11:32:30.99 Lysis2.in :default @11:32:31.03 Archive.setRawPosition! 50 @11:32:31.21 Lysis1.seek :home @11:32:31.29 Archive.seek :home @11:32:31.45 PinPort.setRawPosition! 3150 @11:32:31.52 PinPort.seek :home @11:32:31.69 Archive.in :default @11:32:31.72 Lysis1.in :default @11:32:33.93 Toroid.setRawPosition! 0 @11:32:34.00 Toroid.seek :home @11:32:34.11 Toroid.in :default @11:32:35.93 PinPort.in :default @11:32:36.29 Toroid.seek :clear @11:32:37.00 PinPort.in :unlocking @11:32:37.09 PinPort.seek :clear @11:32:40.45 Air.seek :clear @11:32:40.59 Lysis1.seek :clear @11:32:41.34 Lysis2.seek :clear @11:32:42.16 Archive.seek :clear @11:32:42.96 Carousel.in :default @11:32:43.10 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 176.95ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.59psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @11:35:24.66 -> lysis :microSPR_phyto @11:37:01.96 -> Power.sampler :ON @11:37:01.98 Gate.power :sampler,:ON true @11:37:38.79 -> Power.sampler :OFF @11:37:38.82 Gate.power :sampler,:OFF false @11:39:14.84 -> start {fire lysis} # @11:39:15.76 Toroid.seek :clear @11:39:15.96 Air.seek :clear @11:39:16.07 Lysis1.seek :clear @11:39:16.19 Lysis2.seek :clear @11:39:16.31 Archive.seek :clear @11:39:16.45 Carousel.in :default @11:39:16.60 Carousel.home.seek 22 @11:39:16.91 Selecting Cartridge 22 @11:39:17.25 Carousel.seek 22 @11:39:17.56 Carousel.reconfigure :align @11:39:17.71 Lysis1.in :fast @11:39:17.80 Lysis1.seek :locked @11:39:20.48 Plunge.seek :locked @11:39:20.68 Air.in :fast @11:39:20.77 Air.seek :locked @11:39:21.86 Carousel.reconfigure :locked @11:39:22.04 Powering cartridge bus @11:39:24.73 Gate.setIR 1750,5000,0,6 @11:39:24.86 Gate.setIR 0,5000,0,6 @11:39:24.95 PuckHeater.configure :default @11:39:25.04 Gate.power :heat,:ON,limit to 5000mA @11:39:25.69 Mounted lysis Cartridge board#J59951 @11:39:25.75 Using default cartridge heat loss curve for HeatingFunctionInCarousel @11:39:26.17 Preparing to filter microSPR_phyto cartridge in slot 22 @11:39:26.49 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:39:33.11 Intake Head Pressure tare is 1% of full scale at 28C (drifted 6%) @11:39:33.13 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:39:33.18 PhaseSeparator.in :filling @11:39:33.26 PhaseSeparator.hold @11:39:33.36 Gate.power :sampler,:ON @11:39:40.81 Head pressure reached 1.70psi in 8 seconds @11:39:40.85 Air.in :default @11:39:41.03 Air.seek :sealed @11:39:41.07 Priming sample loop w/200ml, bypass w/50ml @11:39:41.15 SamplePump.setPosition! 0ml @11:39:41.24 Exhaust.close @11:39:41.37 Bypass.open @11:39:43.71 Intake.open @11:39:45.31 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:39:47.93 Vacuum tare is 54% of full scale at 28C (drifted 2%) @11:39:48.09 Abs.in :default @11:39:48.17 Abs.hold @11:39:48.27 PhaseSeparator.in :full @11:39:48.39 PhaseSeparator.hold @11:39:48.65 Waiting for sampler to finish priming... @11:39:48.67 delayUntil "11:40:33.38" @11:40:33.45 SamplePump.in :start @11:40:33.55 SamplePump.seek 50ml,3:50 @11:40:34.14 SamplePump.configure :prime @11:41:39.53 Abs.stop @11:41:39.61 PhaseSeparator.stop @11:41:39.68 SamplePump.setPosition! 0ml @11:41:39.76 Exhaust.open @11:41:42.01 Bypass.close @11:41:44.46 Abs.in :default @11:41:44.55 Abs.hold @11:41:44.65 PhaseSeparator.hold @11:41:45.49 SamplePump.in :start @11:41:45.59 SamplePump.seek 200ml,13:50 @11:41:46.18 SamplePump.configure :prime @11:46:08.23 Abs.stop @11:46:08.31 PhaseSeparator.stop @11:46:08.44 Bypass.open @11:46:09.99 Intake.close @11:46:11.63 Toroid.in :hold @11:46:11.72 Toroid.seek :crack @11:46:14.22 Toroid.seek :cartridge @11:46:16.39 Toroid.in :default @11:46:16.48 Toroid.seek :clear @11:46:20.52 Pressurizing cartridge to 14psi above 13.6psia @11:46:20.54 SamplePump.setPosition! 0ml @11:46:20.63 Intake.open @11:46:22.11 Bypass.close @11:46:23.63 Exhaust.close @11:46:25.32 Abs.in :default @11:46:25.40 Abs.hold @11:46:25.50 PhaseSeparator.hold @11:46:25.76 SamplePump.in :charge @11:46:25.86 SamplePump.seek 2.3ml,38 seconds @11:46:38.54 Abs.stop @11:46:38.62 PhaseSeparator.stop @11:46:38.72 Intake.close @11:46:40.36 Pressurized cartridge with 0.664ml to 15.0psi @11:46:40.38 Isolated @25.2psia (11.7psi) @11:46:40.44 Verifying cartridge holds at least 0.65..0.85 of 25.2psia @11:46:40.51 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:46:45.19 Sampling 100.0ml @11:46:45.21 SamplePump.setPosition! 0ml @11:46:45.31 Exhaust.open @11:46:46.84 Intake.open @11:46:48.53 Abs.in :default @11:46:48.61 Abs.hold @11:46:48.71 PhaseSeparator.hold @11:46:49.61 SamplePump.in :start @11:46:49.71 SamplePump.seek 100ml,6:04 @11:46:50.32 SamplePump.configure :sample @11:46:52.71 Cartridge 22 filtering 2/100ml at 0.76ml/s with 7.9psi vacuum @11:47:52.77 Cartridge 22 filtering 45/100ml at 0.71ml/s with 13.2psi vacuum @11:48:52.77 Cartridge 22 filtering 87/100ml at 0.71ml/s with 13.0psi vacuum @11:49:11.34 Abs.stop @11:49:11.42 PhaseSeparator.stop @11:49:11.52 Intake.close @11:49:11.59 Exhaust.close @11:49:11.86 Toroid.in :hold @11:49:11.95 Toroid.seek :bypass @11:49:15.57 Toroid.in :default @11:49:15.66 Toroid.seek :clear @11:49:19.26 Sampled 100.0ml @11:49:20.93 SPR.command "read_sequences" @11:49:21.58 SPR.command "stats_on" @11:49:21.76 SPR.command "start 0 1" @11:49:22.41 Gate.power :sampler,:OFF @11:49:22.46 SPR.start /var/log/esp/spr26aug0111h.spr.bz2 @11:49:24.43 Handoff.select :waste @11:49:24.97 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:49:31.64 Syringe tare is 50% of full scale at 29C (drifted -1%) @11:49:31.68 PinPort.in :locking @11:49:31.78 PinPort.seek :locked @11:49:32.65 Archive.in :fast @11:49:32.76 Lysis2.in :fast @11:49:32.86 Lysis1.seek 1250uL @11:49:32.92 Archive.seek :empty @11:49:33.08 Lysis2.seek 950uL @11:49:41.49 Evacuating puck with filter bubble point of 20psi @11:49:41.52 PinPort.seek :locked @11:49:41.66 Handoff.select :waste @11:49:42.25 Plunge.seek :puckBottom @11:49:43.64 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:49:51.75 Air syringe pumping up to 1 strokes to reach 4psi @11:49:51.77 Air stroke #1 of 1 @11:49:51.91 Air.in :pressurize @11:49:51.99 Air.seek :pressurized @11:49:57.28 0.0psi after pressurize @11:49:57.31 Air.in :compress @11:49:57.44 Air.seek :evac @11:50:02.98 Reached 1.6 of 4.0psi after pumping 1711uL (0.96 strokes) @11:50:03.00 Air.in :default @11:50:03.17 Air.hold @11:50:03.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:50:15.47 Stabilized at 0.1psi after evac to waste @11:50:15.48 Handoff.select :blocked @11:50:16.03 Plunge.seek :puckTop @11:50:16.95 Air syringe pumping up to 8 strokes to reach 20psi @11:50:16.97 Air stroke #1 of 8 @11:50:17.12 Air.in :compress @11:50:17.20 Air.seek :evac @11:50:18.51 1.8psi after compress @11:50:29.61 3.1psi after compress (stable) @11:50:29.64 Air.in :gulp @11:50:29.74 Air.seek :topVent @11:50:36.24 3.0psi after gulp @11:50:42.36 3.2psi after gulp (stable) @11:50:42.38 Air stroke #2 of 8 @11:50:42.55 Air.in :pressurize @11:50:42.64 Air.seek :pressurized @11:50:49.06 3.4psi after pressurize @11:50:49.14 Air.in :compress @11:50:49.25 Air.seek :evac @11:50:55.90 14.3psi after compress @11:50:59.14 Reached 16.6 of 20.0psi after pumping 3551uL (2.00 strokes) @11:50:59.16 Air.in :default @11:50:59.27 Air.hold @11:50:59.38 Plunge.seek :puckBottom @11:51:00.28 delay 3 seconds @11:51:03.31 Handoff.select :waste @11:51:03.83 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:51:15.43 delay 1:00 @11:52:15.45 Handoff.select :blocked @11:52:15.99 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:52:38.58 Handoff.select :waste @11:52:39.14 0.3psi at evacEnd @11:52:39.17 Plunge.seek :pTop @11:52:40.07 delay 5 seconds @11:52:45.10 Air.in :glide @11:52:45.18 Air.seek :fullVent @11:52:46.85 delay 2 seconds @11:52:48.87 Plunge.seek :pBottom @11:52:49.76 Seawater evacuated, Pressure above/below filter released. @11:52:49.79 Plunge.seek :locked @11:52:51.13 Handoff.select :waste @11:52:51.30 Lysis1.in :slow @11:52:51.39 Lysis1.seek 950uL @11:53:05.50 delay 5 seconds @11:53:10.52 Handoff.select :blocked @11:53:11.05 Plunge.seek :puckTop @11:53:12.04 Lysis1.seek 350uL @11:53:40.06 delay 3 seconds @11:53:43.19 Air.in mix with 2.79934 parts Lysis1 @11:53:43.98 Air.seek :evac @11:53:44.02 Lysis1.seek 190uL @11:53:51.78 Air.in :fast @11:53:51.86 Air.seek :full @11:53:58.39 Lysis1.seek :empty @11:54:07.52 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @13.6psi @11:54:07.55 Plunge.seek :puckBottom @11:54:08.59 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @12.1psi @11:54:08.61 Polling Can every 2:00 @11:54:09.20 PuckHeater.configure :default #ambient 28.6C @11:54:09.28 PuckHeater.seek 81.3C @11:55:31.72 PuckHeater.hold 2:00,81.3C @11:57:31.89 Polling Can every 2:00 @11:57:32.45 PuckHeater.configure :default #ambient 28.7C @11:57:32.54 PuckHeater.seek 80.2C @11:57:32.72 PuckHeater.hold 2:00,80.2C @11:59:32.81 Polling Can every 2:00 @11:59:33.36 PuckHeater.configure :default #ambient 28.6C @11:59:33.44 PuckHeater.hold 8:00,79.2C @12:07:33.38 Lysate cooked @9.1psi @12:07:33.40 Plunge.seek :puckTop @12:07:34.37 Air syringe pumping up to 5 strokes to reach 40psi @12:07:34.38 Air stroke #1 of 5 @12:07:34.54 Air.in :pressurize @12:07:34.63 Air.seek :pressurized @12:07:41.03 9.0psi after pressurize @12:07:41.06 Air.in :compress @12:07:41.18 Air.seek :evac @12:07:47.05 Reached 41.2 of 40.0psi after pumping 1730uL (0.97 strokes) @12:07:47.08 Air.in :default @12:07:47.18 Air.hold @12:07:47.28 delay 2 seconds @12:07:49.31 Air.in :fast @12:07:49.39 Air.seek :full @12:07:55.77 Plunge.seek :puckBottom @12:07:56.74 Lysis1.seek 138uL @12:08:03.80 delay 2 seconds @12:08:05.85 Plunge.seek :puckTop @12:08:06.75 Lysis1.seek :empty @12:08:13.36 Plunge.seek :puckBottom @12:08:14.60 Lysis1.in :extract @12:08:14.74 Lysis1.seek 675uL,nil @12:08:18.46 Air.in :pressurize @12:08:18.63 Air.seek :pressurized @12:08:25.48 10.9psi after pressurize @12:08:25.65 Air.in :compressLysate @12:08:25.83 Air.seek :evac @12:08:40.69 Air.in :default @12:08:40.76 Air.hold @12:08:41.56 Pulled 675uL lysate @13.4psi @12:08:41.92 Lysis1.seek 800uL,nil @12:08:42.21 Air.in :compressLysate @12:08:42.39 Air.seek :evac @12:08:48.59 Air.in :default @12:08:48.66 Air.hold @12:08:48.94 Air.in :glide @12:08:49.03 Air.seek :fullVent @12:08:51.43 Handoff.select :waste @12:08:51.96 Recovered 800uL lysate @15.1psi @12:08:51.98 delay 3 seconds @12:08:55.07 After 3 delay Recovered 800uL lysate @0.5psi @12:08:55.10 Plunge.seek :locked @12:08:56.46 Lysis2.in :DAmix @12:08:56.61 Lysis1.in :DAmix @12:08:57.31 Lysis2.seek 700uL @12:08:57.37 Lysis1.seek 550uL @12:09:32.41 delay 5 seconds @12:09:37.43 Handoff.select SPR @12:09:37.97 SPR.command "start 2 14" @12:09:39.41 SPR stabilized @12:09:39.59 delay 20 seconds @12:09:59.61 SPR.command "start 2 0" @12:10:03.19 Waiting up to 11:00 for SPR to enter state 1 @12:12:01.28 SPR.command "hold_on" @12:12:02.66 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:12:03.38 Lysis2.seek 400uL @12:12:03.43 Lysis1.seek 250uL @12:12:45.41 delay 15 seconds @12:13:00.44 SPR.command "hold_off" @12:13:03.31 Waiting up to 1:00:00 for SPR to enter state 7 @12:28:47.65 -> Power.sampler :ON @12:28:47.68 Gate.power :sampler,:ON true @12:29:18.89 SPRsummary:-7.57RIU,225RIU,230RIU,316RIU,none,none,none after 2531s @12:29:47.07 -> Power.sampler :OFF @12:29:47.09 Gate.power :sampler,:OFF false @12:34:11.08 -> Power.sampler :ON @12:34:11.12 Gate.power :sampler,:ON true @12:36:18.52 -> Power.sampler :OFF @12:36:18.55 Gate.power :sampler,:OFF false @12:43:35.80 SPR analysis completed @12:43:41.29 Ended log spr26aug0111h.spr.bz2 @12:43:41.31 SPR.command "stats_off" @12:43:42.05 Handoff.select :close @12:43:42.59 Gate.power :heat,:OFF @12:43:42.62 Gate.power :cartridge,:OFF @12:43:42.74 PinPort.in :unlocking @12:43:42.82 PinPort.seek :clear @12:43:43.66 Carousel.in :default @12:43:43.81 Carousel.home.seek 22 @12:43:44.15 Plunge.seek :locked @12:43:44.40 Archive.in :unlocking @12:43:44.56 Archive.seek :clear @12:43:44.62 Air.in :unlocking @12:43:44.83 Air.seek :clear @12:43:44.98 Lysis1.in :unlocking @12:43:45.15 Lysis1.seek :clear @12:43:45.24 Lysis2.in :unlocking @12:43:45.36 Lysis2.seek :clear @12:43:49.66 Gate.power :motor,:OFF @12:43:49.69 Gate.power :analytic1,:OFF @12:43:49.73 Gate.power :core,:OFF @12:43:49.76 Gate.power :main,:OFF @12:43:49.79 Safely stopped and ready to power off @13:22:37.51 Session Begun @13:22:44.10 -> exit @13:22:44.13 Session Ended @13:27:33.06 -> Power.sampler :ON @13:27:33.09 Gate.power :sampler,:ON true @13:27:47.21 -> Power.sampler :OFF @13:27:47.24 Gate.power :sampler,:OFF false @13:28:11.20 -> ESP.configure @13:28:11.25 Gate.power :sampler,:OFF,limit to 3500mA @13:28:11.27 Gate.power :skimmer,:OFF,limit to 3500mA @13:28:11.31 Gate.power :main,:ON,limit to 5000mA @13:28:11.34 Gate.power :analytic1,:ON,limit to 4000mA @13:28:11.45 Powering core bus @13:28:13.79 SPR Module 1, FW Rev. B, FPGA Rev. C @13:28:14.23 Plunge.configure :default @13:28:14.37 SamplePump.configure :default @13:28:14.49 Toroid.configure :default @13:28:14.61 Carousel.configure :default @13:28:14.71 Abs.configure :default @13:28:14.82 PhaseSeparator.configure :full @13:28:14.92 Archive.configure :default @13:28:15.04 Air.configure :default @13:28:15.12 Lysis1.configure :default @13:28:15.21 Lysis2.configure :default @13:28:15.31 PinPort.configure :default @13:28:15.39 All elf controllers running firmware version 4.94 @13:28:15.42 Gate.power :motor,:ON,limit to 5000mA @13:28:17.93 -> Power.sampler :ON @13:28:17.96 Gate.power :sampler,:ON true @13:30:40.28 -> Head.psi 4.48675748204981 @13:30:43.29 -> Head.psi 4.48938667561408 @13:32:16.57 -> Power.sampler :OFF @13:32:16.60 Gate.power :sampler,:OFF false @13:32:23.62 -> ESP.reready! @13:32:23.68 Plunge.home.jog 0 @13:32:23.74 Toroid.home.jog 0 @13:32:23.80 Carousel.home.jog 0 @13:32:24.01 Archive.home.jog 0 @13:32:24.18 Air.home.jog 0 @13:32:24.26 Lysis1.home.jog 0 @13:32:24.37 Lysis2.home.jog 0 @13:32:24.43 PinPort.home.jog 0 @13:32:25.36 Toroid.in :homing @13:32:25.45 Plunge.in :homing @13:32:25.57 Archive.in :homing @13:32:25.72 Toroid.jog 1355 @13:32:25.76 Plunge.jog 1478 @13:32:25.86 Archive.jog 1609 @13:32:26.15 Lysis2.in :homing @13:32:26.20 Lysis1.in :homing @13:32:26.24 PinPort.in :homing @13:32:26.28 Air.in :homing @13:32:26.54 PinPort.jog 1232 @13:32:26.60 Lysis2.jog 1615 @13:32:26.65 Lysis1.jog 1595 @13:32:26.70 Air.jog 1426 @13:32:27.19 Plunge.setRawPosition! 50 @13:32:27.28 Plunge.seek :home @13:32:27.40 Archive.setRawPosition! 50 @13:32:27.48 Archive.seek :home @13:32:27.61 Plunge.in :default @13:32:27.78 Archive.in :default @13:32:28.04 Air.setRawPosition! 0 @13:32:28.14 Air.seek :home @13:32:28.29 Lysis1.setRawPosition! 50 @13:32:28.36 Air.in :default @13:32:28.41 Lysis2.setRawPosition! 50 @13:32:28.59 Lysis1.seek :home @13:32:28.67 Lysis2.seek :home @13:32:28.86 PinPort.setRawPosition! 3150 @13:32:28.94 PinPort.seek :home @13:32:29.09 Lysis1.in :default @13:32:29.13 Lysis2.in :default @13:32:30.37 Toroid.setRawPosition! 0 @13:32:30.45 Toroid.seek :home @13:32:30.55 Toroid.in :default @13:32:33.34 PinPort.in :default @13:32:34.19 Toroid.seek :clear @13:32:34.44 PinPort.in :unlocking @13:32:34.51 PinPort.seek :clear @13:32:37.94 Air.seek :clear @13:32:38.07 Lysis1.seek :clear @13:32:38.81 Lysis2.seek :clear @13:32:39.63 Archive.seek :clear @13:32:40.44 Carousel.in :default @13:32:40.59 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @-0.02psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @13:32:48.09 -> ESP.shutdown @13:32:48.27 Gate.power :motor,:OFF @13:32:48.30 Gate.power :analytic1,:OFF @13:32:48.39 Gate.power :core,:OFF @13:32:48.41 Gate.power :main,:OFF [:motor,:analytic1,:core,:main] @13:33:12.22 -> MainThread.exit # @13:33:12.42 ThreadError in nadia -- killed thread @13:33:12.49 Session Ended @13:55:14.33EDT01-Aug-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:55:18.10 >BREAK< ESP3 Gateway v1.44 for core bus @13:55:18.13 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:55:22.21 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:55:22.97 Sea pressure (Abs) tare is 6% of full scale @13:55:30.94 Polling Can every 2:00 @13:55:31.08 Waiting for espclients... @13:55:34.65 Session Begun @13:55:43.88 -> Cart.aspects {standard: {extraDilutions: [1,1], lysed: 950, lysed2: 950}} @13:55:51.66 Session Begun @13:55:59.16 -> showlog @13:57:39.53 -> Cart.aspectsOf :standard, extraDilutions:[1], lysed:950, lysed2:950 {extraDilutions: [1], lysed: 950, lysed2: 950} @13:57:48.83 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 950, lysed2: 950}} @13:58:15.67 -> slot {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 100, lysed: 800, spr: "spr26jul3119h.spr.bz2"}, 22 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26aug0111h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3123h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 431.2}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @13:59:15.46 -> exit @13:59:15.48 Session Ended @14:20:17.17 Session Begun @14:20:22.78 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 950, lysed2: 950}} @14:36:28.95 Session Ended @14:36:35.02 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @14:37:37.01 Session Begun @14:37:41.44 -> slot {20 => { Type: :microSPR_phyto, State: :lysing, filtered: 100, lysed: 800, spr: "spr26jul3119h.spr.bz2"}, 22 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26aug0111h.spr.bz2"}, 30 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3123h.spr.bz2"}, 40 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul3118h.spr.bz2"}, 45 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 431.2}, 50 => { Type: :archiveHiBiomass_bac, State: :leaky, filtered: 223.5}, 51 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 55 => { Type: :lyseHold_phyto, State: :processed, filtered: 100, lysed: 800}} @14:38:03.38 -> slot 60..1=>nil {60..1 => nil} @14:38:08.10 -> slot {} @14:38:14.56 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 950, lysed2: 950}} @14:45:45.86 -> slot 50 =>[:standard] {50 => { Type: :unknown, State: :standard}} @14:46:08.40 -> slot 50 =>[:standard, :microSPR_phyto] {50 => { Type: :microSPR_phyto, State: :standard}} @15:02:49.39 Session Ended @15:11:11.45 Session Begun @15:11:13.39 -> slot {50 => { Type: :microSPR_phyto, State: :standard}} @15:12:27.35 -> slot [50..43,14..12} =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] @15:12:27.38 SyntaxError in bill -- compile error bill:2: syntax error, unexpected '}', expecting ']' slot [50..43,14..12} =>[:microSPR_phyto, :standard... ^ @15:13:31.70 -> slot [50..43,14..12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] {[50..43,14..12] => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @15:14:18.46 -> SC.to 47 @15:14:18.52 BusOff in bill -- Storage Carousel (SC) is not powered! @15:14:28.15 -> ESP.ready! @15:14:28.20 Gate.power :sampler,:OFF,limit to 3500mA @15:14:28.24 Gate.power :skimmer,:OFF,limit to 3500mA @15:14:28.27 Gate.power :main,:ON,limit to 5000mA @15:14:28.31 Gate.power :analytic1,:ON,limit to 4000mA @15:14:28.50 Powering core bus @15:14:30.75 SPR Module 1, FW Rev. B, FPGA Rev. C @15:14:31.29 Plunge.configure :default @15:14:31.40 SamplePump.configure :default @15:14:31.51 Toroid.configure :default @15:14:31.62 Carousel.configure :default @15:14:31.73 Abs.configure :default @15:14:31.88 PhaseSeparator.configure :default @15:14:31.99 Archive.configure :default @15:14:32.10 Air.configure :default @15:14:32.18 Lysis1.configure :default @15:14:32.27 Lysis2.configure :default @15:14:32.38 PinPort.configure :default @15:14:32.46 All elf controllers running firmware version 4.94 @15:14:32.49 Gate.power :motor,:ON,limit to 5000mA @15:14:33.00 Toroid.home.jog 0 @15:14:33.12 Carousel.home.jog 0 @15:14:33.20 Plunge.home.jog 0 @15:14:33.73 PinPort.home.jog 0 @15:14:33.87 Lysis2.home.jog 0 @15:14:33.92 Air.home.jog 0 @15:14:33.98 Lysis1.home.jog 0 @15:14:34.11 Archive.home.jog 0 @15:14:35.10 Plunge.in :homing @15:14:35.21 Toroid.in :homing @15:14:35.35 Plunge.jog 434 @15:14:35.38 Toroid.jog 1356 @15:14:35.77 Lysis2.in :homing @15:14:35.85 Air.in :homing @15:14:35.89 PinPort.in :homing @15:14:36.05 Air.jog 1426 @15:14:36.11 Lysis2.jog 1615 @15:14:36.18 PinPort.jog 1227 @15:14:36.43 Plunge.setRawPosition! 50 @15:14:36.48 Lysis1.in :homing @15:14:36.52 Archive.in :homing @15:14:36.72 Plunge.seek :home @15:14:36.75 Lysis1.jog 1597 @15:14:36.81 Archive.jog 1609 @15:14:37.14 Plunge.in :default @15:14:37.35 Air.setRawPosition! 0 @15:14:37.42 Air.seek :home @15:14:37.57 Lysis2.setRawPosition! 50 @15:14:37.60 Air.in :default @15:14:37.74 Lysis2.seek :home @15:14:38.05 PinPort.setRawPosition! 3150 @15:14:38.16 Lysis2.in :default @15:14:38.21 PinPort.seek :home @15:14:38.41 Lysis1.setRawPosition! 50 @15:14:38.44 Archive.setRawPosition! 50 @15:14:38.59 Lysis1.seek :home @15:14:38.63 Archive.seek :home @15:14:38.99 Lysis1.in :default @15:14:39.08 Archive.in :default @15:14:39.94 Toroid.setRawPosition! 0 @15:14:40.01 Toroid.seek :home @15:14:40.12 Toroid.in :default @15:14:42.66 PinPort.in :default @15:14:43.02 Toroid.seek :clear @15:14:43.20 PinPort.in :unlocking @15:14:43.29 PinPort.seek :clear @15:14:46.70 Air.seek :clear @15:14:46.83 Lysis1.seek :clear @15:14:47.57 Lysis2.seek :clear @15:14:48.40 Archive.seek :clear @15:14:49.21 Carousel.in :default @15:14:49.36 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @3.74psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @15:15:13.21 -> SC.to 47 @15:15:13.25 Carousel.seek 47 Storage Carousel (SC) at 47 @15:15:29.06 -> SC.to 55 @15:15:29.10 Carousel.seek 55 Storage Carousel (SC) at 55 @15:16:05.41 -> SC.to 49 @15:16:05.45 Carousel.seek 49 Storage Carousel (SC) at 49 @15:16:14.82 -> SC.to 60 @15:16:14.87 Carousel.seek 60 Storage Carousel (SC) at 60 @15:16:29.62 -> SC.to 53 @15:16:29.67 Carousel.seek 53 Storage Carousel (SC) at 53 @15:16:43.04 -> SC.to 43 @15:16:43.09 Carousel.seek 43 Storage Carousel (SC) at 43 @15:16:52.43 -> SC.to 45 @15:16:52.46 Carousel.seek 45 Storage Carousel (SC) at 45 @15:20:17.35 -> Cart.standard [50,49,48,47,46,45,44,43,14,13,12] @15:25:33.11 -> slots 1..60=>nil {60..1 => nil} @15:25:37.11 -> slots {} @15:27:55.48 -> slot[50,48,46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] @15:27:55.59 SyntaxError in bill -- compile error bill:16: syntax error, unexpected tASSOC, expecting $end ...46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {... ^ @15:28:19.47 -> slot [50,48,46,44,26,24,22,20,14,13,12] =>[:microSPR_phyto, :standard, {lysed:950, lysed2:950}] {[50,48,46,44,26,24,22,20,14..12] => { Type: :microSPR_phyto, State: :standard, lysed: 950, lysed2: 950}} @15:28:33.68 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 950, lysed2: 950}} @15:28:50.82 -> Cart.standard [50,48,46,44,26,24,22,20,14,13,12] @15:29:04.91 -> SC.to 50 @15:29:04.94 Carousel.seek 50 Storage Carousel (SC) at 50 @15:30:50.11 -> SC.to 48 @15:30:50.16 Carousel.seek 48 Storage Carousel (SC) at 48 @15:31:53.36 -> SC.to 46 @15:31:53.40 Carousel.seek 46 Storage Carousel (SC) at 46 @15:32:40.06 -> SC.to 44 @15:32:40.11 Carousel.seek 44 Storage Carousel (SC) at 44 @15:33:21.98 -> SC.to 26 @15:33:22.03 Carousel.seek 26 Storage Carousel (SC) at 26 @15:34:09.36 -> SC.to 24 @15:34:09.40 Carousel.seek 24 Storage Carousel (SC) at 24 @15:35:02.58 -> SC.to 22 @15:35:02.63 Carousel.seek 22 Storage Carousel (SC) at 22 @15:35:40.73 -> SC.to 20 @15:35:40.78 Carousel.seek 20 Storage Carousel (SC) at 20 @15:36:29.42 -> SC.to 14 @15:36:29.47 Carousel.seek 14 Storage Carousel (SC) at 14 @15:37:19.61 -> SC.to 12 @15:37:19.65 Carousel.seek 12 Storage Carousel (SC) at 12 @15:39:31.16 -> SC.to 12 @15:39:31.21 Carousel.seek 12 Storage Carousel (SC) at 12 @15:40:51.42 -> to 12 @15:40:51.74 Carousel.seek 12 @15:40:52.09 Carousel.reconfigure :align @15:40:52.24 Lysis1.in :fast @15:40:52.32 Lysis1.seek :locked @15:40:54.96 Plunge.seek :locked @15:40:55.15 Air.in :fast @15:40:55.23 Air.seek :locked @15:40:56.32 Carousel.reconfigure :locked @15:40:56.50 Powering cartridge bus @15:40:59.16 Gate.setIR 1750,5000,0,6 @15:40:59.30 Gate.setIR 0,5000,0,6 @15:40:59.39 PuckHeater.configure :default @15:40:59.48 Gate.power :heat,:ON,limit to 5000mA @15:40:59.73 Mounted lysis Cartridge board#J59912 @15:40:59.82 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:41:03.87 -> to 13 @15:41:04.47 Gate.power :heat,:OFF @15:41:04.49 Gate.power :cartridge,:OFF @15:41:04.60 Carousel.in :default @15:41:04.75 Carousel.home.seek 12 @15:41:05.30 Air.in :unlocking @15:41:05.46 Air.seek :clear @15:41:06.56 Lysis1.in :unlocking @15:41:06.70 Lysis1.seek :clear @15:41:06.84 Plunge.in :unlocking @15:41:06.94 Plunge.seek :clear @15:41:08.07 Carousel.seek 13 @15:41:08.65 Carousel.reconfigure :align @15:41:08.80 Lysis1.in :fast @15:41:08.89 Lysis1.seek :locked @15:41:11.53 Plunge.in :default @15:41:11.61 Plunge.seek :locked @15:41:11.84 Air.in :fast @15:41:11.92 Air.seek :locked @15:41:13.02 Carousel.reconfigure :locked @15:41:13.20 Powering cartridge bus @15:41:15.90 Gate.setIR 1750,5000,0,6 @15:41:16.03 Gate.setIR 0,5000,0,6 @15:41:16.12 PuckHeater.configure :default @15:41:16.21 Gate.power :heat,:ON,limit to 5000mA @15:41:16.41 Mounted lysis Cartridge board#J59949 @15:41:16.48 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:41:27.80 -> to 14 @15:41:28.00 Gate.power :heat,:OFF @15:41:28.03 Gate.power :cartridge,:OFF @15:41:28.15 Carousel.in :default @15:41:28.29 Carousel.home.seek 13 @15:41:28.74 Air.in :unlocking @15:41:28.92 Air.seek :clear @15:41:30.00 Lysis1.in :unlocking @15:41:30.15 Lysis1.seek :clear @15:41:30.29 Plunge.in :unlocking @15:41:30.37 Plunge.seek :clear @15:41:31.52 Carousel.seek 14 @15:41:32.12 Carousel.reconfigure :align @15:41:32.28 Lysis1.in :fast @15:41:32.36 Lysis1.seek :locked @15:41:35.01 Plunge.in :default @15:41:35.10 Plunge.seek :locked @15:41:35.28 Air.in :fast @15:41:35.36 Air.seek :locked @15:41:36.45 Carousel.reconfigure :locked @15:41:37.02 Powering cartridge bus @15:41:39.69 Gate.setIR 1750,5000,0,6 @15:41:39.82 Gate.setIR 0,5000,0,6 @15:41:40.01 PuckHeater.configure :default @15:41:40.12 Gate.power :heat,:ON,limit to 5000mA @15:41:40.32 Mounted lysis Cartridge board#J59913 @15:41:40.37 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:41:47.78 -> to 20 @15:41:48.01 Gate.power :heat,:OFF @15:41:48.03 Gate.power :cartridge,:OFF @15:41:48.14 Carousel.in :default @15:41:48.29 Carousel.home.seek 14 @15:41:48.74 Air.in :unlocking @15:41:48.91 Air.seek :clear @15:41:50.03 Lysis1.in :unlocking @15:41:50.22 Lysis1.seek :clear @15:41:50.32 Plunge.in :unlocking @15:41:50.43 Plunge.seek :clear @15:41:51.63 Carousel.seek 20 @15:41:53.26 Carousel.reconfigure :align @15:41:53.41 Lysis1.in :fast @15:41:53.49 Lysis1.seek :locked @15:41:56.14 Plunge.in :default @15:41:56.26 Plunge.seek :locked @15:41:56.39 Air.in :fast @15:41:56.47 Air.seek :locked @15:41:57.58 Carousel.reconfigure :locked @15:41:57.76 Powering cartridge bus @15:42:00.44 Gate.setIR 1750,5000,0,6 @15:42:00.58 Gate.setIR 0,5000,0,6 @15:42:00.67 PuckHeater.configure :default @15:42:00.76 Gate.power :heat,:ON,limit to 5000mA @15:42:00.95 Mounted lysis Cartridge board#J59971 @15:42:01.01 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:42:12.08 -> to 22 @15:42:12.27 Gate.power :heat,:OFF @15:42:12.30 Gate.power :cartridge,:OFF @15:42:12.40 Carousel.in :default @15:42:12.56 Carousel.home.seek 20 @15:42:13.03 Air.in :unlocking @15:42:13.16 Air.seek :clear @15:42:14.24 Lysis1.in :unlocking @15:42:14.40 Lysis1.seek :clear @15:42:14.51 Plunge.in :unlocking @15:42:14.62 Plunge.seek :clear @15:42:15.83 Carousel.seek 22 @15:42:16.60 Carousel.reconfigure :align @15:42:16.75 Lysis1.in :fast @15:42:16.83 Lysis1.seek :locked @15:42:19.58 Plunge.in :default @15:42:19.72 Plunge.seek :locked @15:42:19.90 Air.in :fast @15:42:19.98 Air.seek :locked @15:42:21.09 Carousel.reconfigure :locked @15:42:21.26 Powering cartridge bus @15:42:23.93 Gate.setIR 1750,5000,0,6 @15:42:24.06 Gate.setIR 0,5000,0,6 @15:42:24.15 PuckHeater.configure :default @15:42:24.24 Gate.power :heat,:ON,limit to 5000mA @15:42:24.44 Mounted lysis Cartridge board#J59951 @15:42:24.50 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:42:30.43 -> to 24 @15:42:30.62 Gate.power :heat,:OFF @15:42:30.65 Gate.power :cartridge,:OFF @15:42:30.76 Carousel.in :default @15:42:30.90 Carousel.home.seek 22 @15:42:31.37 Air.in :unlocking @15:42:31.51 Air.seek :clear @15:42:32.60 Lysis1.in :unlocking @15:42:32.76 Lysis1.seek :clear @15:42:32.89 Plunge.in :unlocking @15:42:32.98 Plunge.seek :clear @15:42:34.12 Carousel.seek 24 @15:42:34.91 Carousel.reconfigure :align @15:42:35.06 Lysis1.in :fast @15:42:35.14 Lysis1.seek :locked @15:42:37.82 Plunge.in :default @15:42:37.96 Plunge.seek :locked @15:42:38.10 Air.in :fast @15:42:38.19 Air.seek :locked @15:42:39.28 Carousel.reconfigure :locked @15:42:39.48 Powering cartridge bus @15:42:42.17 Gate.setIR 1750,5000,0,6 @15:42:42.32 Gate.setIR 0,5000,0,6 @15:42:42.41 PuckHeater.configure :default @15:42:42.50 Gate.power :heat,:ON,limit to 5000mA @15:42:42.71 Mounted lysis Cartridge board#J59986 @15:42:42.77 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:42:47.53 -> to 26 @15:42:47.73 Gate.power :heat,:OFF @15:42:47.75 Gate.power :cartridge,:OFF @15:42:47.91 Carousel.in :default @15:42:48.06 Carousel.home.seek 24 @15:42:48.51 Air.in :unlocking @15:42:48.69 Air.seek :clear @15:42:49.89 Lysis1.in :unlocking @15:42:49.99 Lysis1.seek :clear @15:42:50.09 Plunge.in :unlocking @15:42:50.71 Plunge.seek :clear @15:42:51.94 Carousel.seek 26 @15:42:52.73 Carousel.reconfigure :align @15:42:52.89 Lysis1.in :fast @15:42:53.01 Slide::Warning in core -- Storage Carousel (SC) reseek at 26 + 3 counts @15:42:53.06 Lysis1.seek :locked @15:42:55.73 Plunge.in :default @15:42:55.84 Plunge.seek :locked @15:42:56.04 Air.in :fast @15:42:56.12 Air.seek :locked @15:42:57.22 Carousel.reconfigure :locked @15:42:57.40 Powering cartridge bus @15:43:00.06 Gate.setIR 1750,5000,0,6 @15:43:00.19 Gate.setIR 0,5000,0,6 @15:43:00.28 PuckHeater.configure :default @15:43:00.37 Gate.power :heat,:ON,limit to 5000mA @15:43:00.57 Mounted lysis Cartridge board#J59943 @15:43:00.62 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:43:45.77 -> to 44 @15:43:46.01 Gate.power :heat,:OFF @15:43:46.03 Gate.power :cartridge,:OFF @15:43:46.15 Carousel.in :default @15:43:46.30 Carousel.home.seek 26 @15:43:46.74 Air.in :unlocking @15:43:46.91 Air.seek :clear @15:43:48.01 Lysis1.in :unlocking @15:43:48.18 Lysis1.seek :clear @15:43:48.28 Plunge.in :unlocking @15:43:48.38 Plunge.seek :clear @15:43:49.59 Carousel.seek 44 @15:43:53.65 Carousel.reconfigure :align @15:43:53.83 Lysis1.in :fast @15:43:53.94 Slide::Warning in core -- Storage Carousel (SC) reseek at 44 + 3 counts (sensed 44 + 4 counts) @15:43:53.97 Lysis1.seek :locked @15:43:56.64 Plunge.in :default @15:43:56.76 Plunge.seek :locked @15:43:56.89 Air.in :fast @15:43:56.98 Air.seek :locked @15:43:58.07 Carousel.reconfigure :locked @15:43:58.25 Powering cartridge bus @15:44:00.92 Gate.setIR 1750,5000,0,6 @15:44:01.05 Gate.setIR 0,5000,0,6 @15:44:01.14 PuckHeater.configure :default @15:44:01.23 Gate.power :heat,:ON,limit to 5000mA @15:44:01.43 Mounted lysis Cartridge board#J59915 @15:44:01.47 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:44:06.94 -> to 46 @15:44:07.15 Gate.power :heat,:OFF @15:44:07.17 Gate.power :cartridge,:OFF @15:44:07.28 Carousel.in :default @15:44:07.43 Carousel.home.seek 44 @15:44:08.45 Air.in :unlocking @15:44:08.57 Air.seek :clear @15:44:09.66 Lysis1.in :unlocking @15:44:09.84 Lysis1.seek :clear @15:44:09.98 Plunge.in :unlocking @15:44:10.08 Plunge.seek :clear @15:44:11.21 Carousel.seek 46 @15:44:12.02 Carousel.reconfigure :align @15:44:12.16 Lysis1.in :fast @15:44:12.25 Lysis1.seek :locked @15:44:14.88 Plunge.in :default @15:44:14.97 Plunge.seek :locked @15:44:15.16 Air.in :fast @15:44:15.24 Air.seek :locked @15:44:16.33 Carousel.reconfigure :locked @15:44:16.52 Powering cartridge bus @15:44:19.19 Gate.setIR 1750,5000,0,6 @15:44:19.32 Gate.setIR 0,5000,0,6 @15:44:19.41 PuckHeater.configure :default @15:44:19.50 Gate.power :heat,:ON,limit to 5000mA @15:44:19.72 Mounted lysis Cartridge board#J59919 @15:44:19.79 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:44:25.31 -> to 48 @15:44:25.51 Gate.power :heat,:OFF @15:44:25.53 Gate.power :cartridge,:OFF @15:44:25.65 Carousel.in :default @15:44:25.84 Carousel.home.seek 46 @15:44:26.30 Air.in :unlocking @15:44:26.45 Air.seek :clear @15:44:27.53 Lysis1.in :unlocking @15:44:27.70 Lysis1.seek :clear @15:44:27.82 Plunge.in :unlocking @15:44:27.95 Plunge.seek :clear @15:44:29.15 Carousel.seek 48 @15:44:29.94 Carousel.reconfigure :align @15:44:30.08 Lysis1.in :fast @15:44:30.17 Lysis1.seek :locked @15:44:32.82 Plunge.in :default @15:44:32.93 Plunge.seek :locked @15:44:33.09 Air.in :fast @15:44:33.17 Air.seek :locked @15:44:34.26 Carousel.reconfigure :locked @15:44:34.45 Powering cartridge bus @15:44:37.11 Gate.setIR 1750,5000,0,6 @15:44:37.25 Gate.setIR 0,5000,0,6 @15:44:37.34 PuckHeater.configure :default @15:44:37.43 Gate.power :heat,:ON,limit to 5000mA @15:44:38.09 Mounted lysis Cartridge board#J59984 @15:44:38.13 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:44:43.09 -> to 50 @15:44:43.29 Gate.power :heat,:OFF @15:44:43.32 Gate.power :cartridge,:OFF @15:44:43.42 Carousel.in :default @15:44:43.57 Carousel.home.seek 48 @15:44:44.13 Air.in :unlocking @15:44:44.31 Air.seek :clear @15:44:45.42 Lysis1.in :unlocking @15:44:45.59 Lysis1.seek :clear @15:44:45.67 Plunge.in :unlocking @15:44:45.85 Plunge.seek :clear @15:44:47.04 Carousel.seek 50 @15:44:47.86 Carousel.reconfigure :align @15:44:48.01 Lysis1.in :fast @15:44:48.09 Lysis1.seek :locked @15:44:50.74 Plunge.in :default @15:44:50.83 Plunge.seek :locked @15:44:51.04 Air.in :fast @15:44:51.12 Air.seek :locked @15:44:52.20 Carousel.reconfigure :locked @15:44:52.39 Powering cartridge bus @15:44:55.05 Gate.setIR 1750,5000,0,6 @15:44:55.18 Gate.setIR 0,5000,0,6 @15:44:55.27 PuckHeater.configure :default @15:44:55.36 Gate.power :heat,:ON,limit to 5000mA @15:44:55.56 Mounted lysis Cartridge board#J59963 @15:44:55.62 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @15:45:10.96 -> Cartridge.unlock @15:45:11.01 Gate.power :heat,:OFF @15:45:11.03 Gate.power :cartridge,:OFF @15:45:11.15 Carousel.in :default @15:45:11.30 Carousel.home.seek 50 @15:45:11.77 Air.in :unlocking @15:45:11.98 Air.seek :clear @15:45:13.06 Lysis1.in :unlocking @15:45:13.21 Lysis1.seek :clear @15:45:13.37 Plunge.in :unlocking @15:45:13.44 Plunge.seek :clear @15:45:56.24 -> Cart.aspets @15:45:56.29 NoMethodError in bill -- undefined method `aspets' for Cartridge @15:46:00.31 -> Cart.aspects {standard: {extraDilutions: [1], lysed: 950, lysed2: 950}} @15:52:31.92 -> start {fireAll "standard"} # @15:52:32.44 Cartridge group: standard @15:52:33.90 Toroid.seek :clear @15:52:34.11 Air.in :default @15:52:34.20 Air.seek :clear @15:52:34.31 Lysis1.in :default @15:52:34.40 Lysis1.seek :clear @15:52:34.52 Lysis2.seek :clear @15:52:34.66 Archive.seek :clear @15:52:34.80 Carousel.in :default @15:52:34.97 Carousel.home.seek 50 @15:52:35.28 Selecting Cartridge 50 @15:52:35.64 Carousel.seek 50 @15:52:35.95 Carousel.reconfigure :align @15:52:36.12 Lysis1.in :fast @15:52:36.22 Lysis1.seek :locked @15:52:39.00 Plunge.in :default @15:52:39.24 Plunge.seek :locked @15:52:39.48 Air.in :fast @15:52:39.61 Air.seek :locked @15:52:41.01 Session Begun @15:52:41.17 Carousel.reconfigure :locked @15:52:41.38 Powering cartridge bus @15:52:44.07 Gate.setIR 1750,5000,0,6 @15:52:44.20 Gate.setIR 0,5000,0,6 @15:52:44.31 PuckHeater.configure :default @15:52:44.42 Gate.power :heat,:ON,limit to 5000mA @15:52:44.65 Mounted lysis Cartridge board#J59963 @15:52:44.73 Using default cartridge heat loss curve for HeatingFunctionInCarousel @15:52:46.35 SPR.command "read_sequences" @15:52:47.13 SPR.command "stats_on" @15:52:47.32 SPR.command "start 0 1" @15:52:48.04 SPR.start /var/log/esp/spr26aug0115h.spr.bz2 @15:52:49.99 Waiting for baseline SPR stabilization @15:52:50.01 Handoff.select :waste @15:52:50.59 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:52:57.22 Syringe tare is 50% of full scale at 24C (drifted -0%) @15:52:57.29 PinPort.in :locking @15:52:57.41 PinPort.seek :locked @15:52:58.31 Archive.in :fast @15:52:58.44 Lysis2.in :fast @15:52:58.50 Lysis1.seek 950uL @15:52:58.56 Archive.seek :empty @15:52:58.84 Lysis2.seek 950uL @15:53:07.10 delayUntil "16:02:49.53" @15:57:32.50 -> exit @15:57:32.53 Session Ended @16:02:49.54 Lysis2.in :DAmix @16:02:49.69 Lysis1.in :DAmix @16:02:50.32 Lysis2.seek 700uL @16:02:50.42 Lysis1.seek 700uL @16:03:25.43 delay 5 seconds @16:03:30.45 Handoff.select SPR @16:03:30.97 SPR.command "start 2 14" @16:03:32.21 SPR stabilized @16:03:32.40 delay 20 seconds @16:03:52.43 SPR.command "start 2 0" @16:03:55.04 Waiting up to 11:00 for SPR to enter state 1 @16:05:52.70 SPR.command "hold_on" @16:05:54.60 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:05:55.26 Lysis2.seek 400uL @16:05:55.34 Lysis1.seek 400uL @16:06:37.28 delay 15 seconds @16:06:52.30 SPR.command "hold_off" @16:06:55.70 Waiting up to 1:00:00 for SPR to enter state 7 @16:23:10.32 SPRsummary:6.05RIU,271RIU,294RIU,385RIU after 1924s @16:37:27.36 delay 20 seconds @16:37:47.73 SPR.command "start 2 0" @16:37:50.47 Waiting up to 11:00 for SPR to enter state 1 @16:39:48.14 SPR.command "hold_on" @16:39:49.53 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @16:39:50.18 Lysis2.seek 100uL @16:39:50.25 Lysis1.seek 100uL @16:40:32.23 delay 15 seconds @16:40:47.26 SPR.command "hold_off" @16:40:50.17 Waiting up to 1:00:00 for SPR to enter state 7 @17:11:22.66 SPR analysis completed @17:11:23.26 Ended log spr26aug0115h.spr.bz2 @17:11:23.28 SPR.command "stats_off" @17:11:24.53 Handoff.select :close @17:11:25.07 Gate.power :heat,:OFF @17:11:25.10 Gate.power :cartridge,:OFF @17:11:25.22 PinPort.in :unlocking @17:11:25.30 PinPort.seek :clear @17:11:26.16 Carousel.in :default @17:11:26.32 Carousel.home.seek 50 @17:11:26.68 Plunge.seek :locked @17:11:26.89 Archive.in :unlocking @17:11:27.03 Archive.seek :clear @17:11:27.19 Air.in :unlocking @17:11:27.34 Air.seek :clear @17:11:27.46 Lysis1.in :unlocking @17:11:28.13 Lysis1.seek :clear @17:11:28.27 Lysis2.in :unlocking @17:11:28.41 Lysis2.seek :clear @17:11:33.06 Gate.power :motor,:OFF @17:11:33.08 Gate.power :analytic1,:OFF @17:11:33.13 Gate.power :core,:OFF @17:11:33.17 Gate.power :main,:OFF @17:11:33.20 Safely stopped and ready to power off @17:11:33.86 Gate.power :sampler,:OFF,limit to 3500mA @17:11:33.89 Gate.power :skimmer,:OFF,limit to 3500mA @17:11:36.98 Gate.power :main,:ON,limit to 5000mA @17:11:37.00 Gate.power :analytic1,:ON,limit to 4000mA @17:11:37.13 Powering core bus @17:11:39.46 SPR Module 1, FW Rev. B, FPGA Rev. C @17:11:39.95 Plunge.configure :default @17:11:40.06 SamplePump.configure :default @17:11:40.16 Toroid.configure :default @17:11:40.27 Carousel.configure :default @17:11:40.37 Abs.configure :default @17:11:40.48 PhaseSeparator.configure :default @17:11:40.60 Archive.configure :default @17:11:40.70 Air.configure :default @17:11:40.78 Lysis1.configure :default @17:11:40.86 Lysis2.configure :default @17:11:40.97 PinPort.configure :default @17:11:41.04 All elf controllers running firmware version 4.94 @17:11:41.08 Gate.power :motor,:ON,limit to 5000mA @17:11:41.56 Plunge.home.jog 0 @17:11:42.25 Carousel.home.jog 0 @17:11:42.35 Lysis2.home.jog 0 @17:11:42.40 Air.home.jog 0 @17:11:42.48 PinPort.home.jog 0 @17:11:42.55 Lysis1.home.jog 0 @17:11:42.59 Toroid.home.jog 0 @17:11:42.63 Archive.home.jog 0 @17:11:43.56 Plunge.in :homing @17:11:43.87 Plunge.jog 1463 @17:11:44.12 PinPort.in :homing @17:11:44.17 Lysis2.in :homing @17:11:44.22 Air.in :homing @17:11:44.27 Toroid.in :homing @17:11:44.31 Lysis1.in :homing @17:11:44.38 Archive.in :homing @17:11:44.64 PinPort.jog 1244 @17:11:44.75 Lysis2.jog 1619 @17:11:44.81 Toroid.jog 1360 @17:11:44.85 Air.jog 1433 @17:11:44.91 Lysis1.jog 1602 @17:11:44.96 Archive.jog 1616 @17:11:45.44 Plunge.setRawPosition! 50 @17:11:45.52 Plunge.seek :home @17:11:46.33 Plunge.in :default @17:11:46.48 Lysis2.setRawPosition! 50 @17:11:46.54 Air.setRawPosition! 0 @17:11:46.67 Lysis2.seek :home @17:11:46.70 Air.seek :home @17:11:46.96 Lysis1.setRawPosition! 50 @17:11:47.01 Air.in :default @17:11:47.06 PinPort.setRawPosition! 3150 @17:11:47.10 Archive.setRawPosition! 50 @17:11:47.30 Lysis1.seek :home @17:11:47.39 PinPort.seek :home @17:11:47.42 Archive.seek :home @17:11:47.71 Lysis2.in :default @17:11:47.88 Lysis1.in :default @17:11:47.99 Archive.in :default @17:11:49.72 Toroid.setRawPosition! 0 @17:11:49.84 Toroid.seek :home @17:11:49.96 Toroid.in :default @17:11:51.90 PinPort.in :default @17:11:52.27 Toroid.seek :clear @17:11:52.46 PinPort.in :unlocking @17:11:52.54 PinPort.seek :clear @17:11:55.92 Air.seek :clear @17:11:56.06 Lysis1.seek :clear @17:11:56.81 Lysis2.seek :clear @17:11:57.63 Archive.seek :clear @17:11:58.45 Carousel.in :default @17:11:58.60 Carousel.home.seek 50 @17:11:58.89 Selecting Cartridge 48 @17:11:59.23 Carousel.seek 48 @17:12:00.02 Carousel.reconfigure :align @17:12:00.17 Lysis1.in :fast @17:12:00.26 Lysis1.seek :locked @17:12:02.91 Plunge.seek :locked @17:12:03.12 Air.in :fast @17:12:03.20 Air.seek :locked @17:12:04.30 Carousel.reconfigure :locked @17:12:04.48 Powering cartridge bus @17:12:07.15 Gate.setIR 1750,5000,0,6 @17:12:07.29 Gate.setIR 0,5000,0,6 @17:12:07.38 PuckHeater.configure :default @17:12:07.48 Gate.power :heat,:ON,limit to 5000mA @17:12:07.71 Mounted lysis Cartridge board#J59984 @17:12:07.80 Using default cartridge heat loss curve for HeatingFunctionInCarousel @17:12:09.92 SPR.command "read_sequences" @17:12:10.57 SPR.command "stats_on" @17:12:10.76 SPR.command "start 0 1" @17:12:11.46 SPR.start /var/log/esp/spr26aug0117h.spr.bz2 @17:12:13.42 Waiting for baseline SPR stabilization @17:12:13.45 Handoff.select :waste @17:12:14.01 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:12:26.64 Syringe tare is 50% of full scale at 24C (drifted -0%) @17:12:26.67 PinPort.in :locking @17:12:26.77 PinPort.seek :locked @17:12:27.64 Archive.in :fast @17:12:27.71 Lysis2.in :fast @17:12:27.81 Lysis1.seek 950uL @17:12:28.01 Lysis2.seek 950uL @17:12:28.08 Archive.seek :empty @17:12:36.57 delayUntil "17:22:13.00" @17:22:13.02 Lysis2.in :DAmix @17:22:13.19 Lysis1.in :DAmix @17:22:13.80 Lysis1.seek 700uL @17:22:13.83 Lysis2.seek 700uL @17:22:48.85 delay 5 seconds @17:22:53.87 Handoff.select SPR @17:22:54.39 SPR.command "start 2 14" @17:22:55.65 SPR stabilized @17:22:55.88 delay 20 seconds @17:23:15.90 SPR.command "start 2 0" @17:23:18.48 Waiting up to 11:00 for SPR to enter state 1 @17:25:16.15 SPR.command "hold_on" @17:25:17.53 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:25:18.23 Lysis2.seek 400uL @17:25:18.29 Lysis1.seek 400uL @17:26:00.27 delay 15 seconds @17:26:15.30 SPR.command "hold_off" @17:26:18.18 Waiting up to 1:00:00 for SPR to enter state 7 @17:42:33.76 SPRsummary:4.73RIU,262RIU,252RIU,353RIU after 1924s @17:56:50.81 delay 20 seconds @17:57:10.83 SPR.command "start 2 0" @17:57:13.90 Waiting up to 11:00 for SPR to enter state 1 @17:59:11.58 SPR.command "hold_on" @17:59:13.49 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:59:14.23 Lysis2.seek 100uL @17:59:14.28 Lysis1.seek 100uL @17:59:56.26 delay 15 seconds @18:00:11.28 SPR.command "hold_off" @18:00:14.56 Waiting up to 1:00:00 for SPR to enter state 7 @18:30:46.10 SPR analysis completed @18:30:46.76 Ended log spr26aug0117h.spr.bz2 @18:30:46.78 SPR.command "stats_off" @18:30:47.96 Handoff.select :close @18:30:48.50 Gate.power :heat,:OFF @18:30:48.52 Gate.power :cartridge,:OFF @18:30:48.66 PinPort.in :unlocking @18:30:48.74 PinPort.seek :clear @18:30:49.60 Carousel.in :default @18:30:49.75 Carousel.home.seek 48 @18:30:50.16 Plunge.seek :locked @18:30:50.38 Archive.in :unlocking @18:30:50.54 Archive.seek :clear @18:30:50.62 Air.in :unlocking @18:30:50.80 Air.seek :clear @18:30:50.96 Lysis1.in :unlocking @18:30:51.12 Lysis1.seek :clear @18:30:51.23 Lysis2.in :unlocking @18:30:51.35 Lysis2.seek :clear @18:30:55.99 Gate.power :motor,:OFF @18:30:56.03 Gate.power :analytic1,:OFF @18:30:56.06 Gate.power :core,:OFF @18:30:56.10 Gate.power :main,:OFF @18:30:56.13 Safely stopped and ready to power off @18:30:56.79 Gate.power :sampler,:OFF,limit to 3500mA @18:30:56.82 Gate.power :skimmer,:OFF,limit to 3500mA @18:30:59.90 Gate.power :main,:ON,limit to 5000mA @18:30:59.94 Gate.power :analytic1,:ON,limit to 4000mA @18:31:00.03 Powering core bus @18:31:02.42 SPR Module 1, FW Rev. B, FPGA Rev. C @18:31:02.79 Plunge.configure :default @18:31:02.91 SamplePump.configure :default @18:31:03.01 Toroid.configure :default @18:31:03.13 Carousel.configure :default @18:31:03.24 Abs.configure :default @18:31:03.34 PhaseSeparator.configure :default @18:31:03.44 Archive.configure :default @18:31:03.56 Air.configure :default @18:31:03.64 Lysis1.configure :default @18:31:03.72 Lysis2.configure :default @18:31:03.87 PinPort.configure :default @18:31:03.95 All elf controllers running firmware version 4.94 @18:31:03.98 Gate.power :motor,:ON,limit to 5000mA @18:31:04.25 Plunge.home.jog 0 @18:31:04.91 Lysis1.home.jog 0 @18:31:05.07 Archive.home.jog 0 @18:31:05.13 Toroid.home.jog 0 @18:31:05.20 Lysis2.home.jog 0 @18:31:05.23 Air.home.jog 0 @18:31:05.27 Carousel.home.jog 0 @18:31:05.35 PinPort.home.jog 0 @18:31:06.25 Plunge.in :homing @18:31:06.51 Plunge.jog 1487 @18:31:06.73 Air.in :homing @18:31:06.84 Lysis2.in :homing @18:31:06.87 Toroid.in :homing @18:31:06.93 Lysis1.in :homing @18:31:06.97 Archive.in :homing @18:31:07.02 PinPort.in :homing @18:31:07.31 Air.jog 1431 @18:31:07.40 Lysis2.jog 1619 @18:31:07.48 Lysis1.jog 1602 @18:31:07.54 Archive.jog 1619 @18:31:07.58 Toroid.jog 1360 @18:31:07.63 PinPort.jog 1241 @18:31:08.17 Plunge.setRawPosition! 50 @18:31:08.25 Plunge.seek :home @18:31:08.55 Plunge.in :default @18:31:08.87 Air.setRawPosition! 0 @18:31:08.95 Air.seek :home @18:31:09.08 Lysis2.setRawPosition! 50 @18:31:09.18 Lysis2.seek :home @18:31:09.34 Lysis1.setRawPosition! 50 @18:31:09.37 Air.in :default @18:31:09.41 Archive.setRawPosition! 50 @18:31:09.57 Lysis1.seek :home @18:31:09.66 Archive.seek :home @18:31:09.90 PinPort.setRawPosition! 3150 @18:31:09.92 Lysis2.in :default @18:31:10.49 PinPort.seek :home @18:31:10.75 Archive.in :default @18:31:10.78 Lysis1.in :default @18:31:12.46 Toroid.setRawPosition! 0 @18:31:12.54 Toroid.seek :home @18:31:12.65 Toroid.in :default @18:31:15.00 PinPort.in :default @18:31:15.38 Toroid.seek :clear @18:31:15.57 PinPort.in :unlocking @18:31:15.66 PinPort.seek :clear @18:31:19.08 Air.seek :clear @18:31:19.20 Lysis1.seek :clear @18:31:19.95 Lysis2.seek :clear @18:31:20.78 Archive.seek :clear @18:31:21.60 Carousel.in :default @18:31:21.77 Carousel.home.seek 48 @18:31:22.09 Selecting Cartridge 46 @18:31:22.44 Carousel.seek 46 @18:31:23.22 Carousel.reconfigure :align @18:31:23.39 Lysis1.in :fast @18:31:23.46 Lysis1.seek :locked @18:31:26.14 Plunge.seek :locked @18:31:26.34 Air.in :fast @18:31:26.41 Air.seek :locked @18:31:27.50 Carousel.reconfigure :locked @18:31:27.68 Powering cartridge bus @18:31:30.35 Gate.setIR 1750,5000,0,6 @18:31:30.49 Gate.setIR 0,5000,0,6 @18:31:30.59 PuckHeater.configure :default @18:31:30.69 Gate.power :heat,:ON,limit to 5000mA @18:31:30.89 Mounted lysis Cartridge board#J59919 @18:31:30.94 Using default cartridge heat loss curve for HeatingFunctionInCarousel @18:31:32.54 SPR.command "read_sequences" @18:31:32.61 SPR.command "stats_on" @18:31:32.78 SPR.command "start 0 1" @18:31:33.48 SPR.start /var/log/esp/spr26aug0118h.spr.bz2 @18:31:35.40 Waiting for baseline SPR stabilization @18:31:35.41 Handoff.select :waste @18:31:36.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:31:43.17 Syringe tare is 51% of full scale at 24C (drifted -0%) @18:31:43.20 PinPort.in :locking @18:31:43.30 PinPort.seek :locked @18:31:44.17 Archive.in :fast @18:31:44.22 Lysis2.in :fast @18:31:44.35 Lysis1.seek 950uL @18:31:44.48 Archive.seek :empty @18:31:44.63 Lysis2.seek 950uL @18:31:52.95 delayUntil "18:41:34.96" @18:41:34.98 Lysis2.in :DAmix @18:41:35.12 Lysis1.in :DAmix @18:41:35.79 Lysis2.seek 700uL @18:41:35.85 Lysis1.seek 700uL @18:42:10.86 delay 5 seconds @18:42:15.89 Handoff.select SPR @18:42:16.42 SPR.command "start 2 14" @18:42:17.62 SPR stabilized @18:42:17.85 delay 20 seconds @18:42:37.87 SPR.command "start 2 0" @18:42:40.45 Waiting up to 11:00 for SPR to enter state 1 @18:44:38.11 SPR.command "hold_on" @18:44:39.50 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:44:40.23 Lysis2.seek 400uL @18:44:40.29 Lysis1.seek 400uL @18:45:22.27 delay 15 seconds @18:45:37.29 SPR.command "hold_off" @18:45:40.16 Waiting up to 1:00:00 for SPR to enter state 7 @19:01:55.73 SPRsummary:3.17RIU,263RIU,253RIU,348RIU after 1924s @19:16:12.79 delay 20 seconds @19:16:32.81 SPR.command "start 2 0" @19:16:35.89 Waiting up to 11:00 for SPR to enter state 1 @19:18:33.56 SPR.command "hold_on" @19:18:34.93 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:18:35.67 Lysis2.seek 100uL @19:18:35.71 Lysis1.seek 100uL @19:19:17.70 delay 15 seconds @19:19:32.72 SPR.command "hold_off" @19:19:35.59 Waiting up to 1:00:00 for SPR to enter state 7 @19:50:08.07 SPR analysis completed @19:50:08.75 Ended log spr26aug0118h.spr.bz2 @19:50:08.77 SPR.command "stats_off" @19:50:09.90 Handoff.select :close @19:50:10.44 Gate.power :heat,:OFF @19:50:10.47 Gate.power :cartridge,:OFF @19:50:10.60 PinPort.in :unlocking @19:50:10.68 PinPort.seek :clear @19:50:11.53 Carousel.in :default @19:50:11.69 Carousel.home.seek 46 @19:50:12.06 Plunge.seek :locked @19:50:12.27 Archive.in :unlocking @19:50:12.41 Archive.seek :clear @19:50:12.54 Air.in :unlocking @19:50:12.70 Air.seek :clear @19:50:12.83 Lysis1.in :unlocking @19:50:12.99 Lysis1.seek :clear @19:50:13.13 Lysis2.in :unlocking @19:50:13.23 Lysis2.seek :clear @19:50:17.91 Gate.power :motor,:OFF @19:50:17.94 Gate.power :analytic1,:OFF @19:50:17.98 Gate.power :core,:OFF @19:50:18.02 Gate.power :main,:OFF @19:50:18.06 Safely stopped and ready to power off @19:50:18.28 Gate.power :sampler,:OFF,limit to 3500mA @19:50:18.30 Gate.power :skimmer,:OFF,limit to 3500mA @19:50:22.24 Gate.power :main,:ON,limit to 5000mA @19:50:22.39 Gate.power :analytic1,:ON,limit to 4000mA @19:50:22.50 Powering core bus @19:50:24.84 SPR Module 1, FW Rev. B, FPGA Rev. C @19:50:25.31 Plunge.configure :default @19:50:25.43 SamplePump.configure :default @19:50:25.53 Toroid.configure :default @19:50:25.64 Carousel.configure :default @19:50:25.74 Abs.configure :default @19:50:25.90 PhaseSeparator.configure :default @19:50:26.00 Archive.configure :default @19:50:26.11 Air.configure :default @19:50:26.20 Lysis1.configure :default @19:50:26.28 Lysis2.configure :default @19:50:26.39 PinPort.configure :default @19:50:26.48 All elf controllers running firmware version 4.94 @19:50:26.51 Gate.power :motor,:ON,limit to 5000mA @19:50:26.88 Plunge.home.jog 0 @19:50:27.57 PinPort.home.jog 0 @19:50:27.63 Lysis1.home.jog 0 @19:50:27.68 Archive.home.jog 0 @19:50:27.71 Toroid.home.jog 0 @19:50:27.76 Lysis2.home.jog 0 @19:50:27.87 Air.home.jog 0 @19:50:27.90 Carousel.home.jog 0 @19:50:28.78 Plunge.in :homing @19:50:29.00 Plunge.jog 1490 @19:50:29.26 PinPort.in :homing @19:50:29.34 Lysis2.in :homing @19:50:29.39 Air.in :homing @19:50:29.43 Archive.in :homing @19:50:29.46 Toroid.in :homing @19:50:29.52 Lysis1.in :homing @19:50:29.83 PinPort.jog 1239 @19:50:29.96 Lysis2.jog 1620 @19:50:30.02 Lysis1.jog 1601 @19:50:30.06 Air.jog 1431 @19:50:30.10 Toroid.jog 1349 @19:50:30.21 Archive.jog 1620 @19:50:30.59 Plunge.setRawPosition! 50 @19:50:30.67 Plunge.seek :home @19:50:30.97 Plunge.in :default @19:50:31.57 Air.setRawPosition! 0 @19:50:31.66 Air.seek :home @19:50:31.87 Lysis2.setRawPosition! 50 @19:50:31.90 Lysis1.setRawPosition! 50 @19:50:31.93 Archive.setRawPosition! 50 @19:50:32.00 Air.in :default @19:50:32.18 Lysis2.seek :home @19:50:32.21 Lysis1.seek :home @19:50:32.41 Archive.seek :home @19:50:33.09 PinPort.setRawPosition! 3150 @19:50:33.21 Lysis2.in :default @19:50:33.28 Lysis1.in :default @19:50:33.40 PinPort.seek :home @19:50:33.64 Archive.in :default @19:50:34.96 Toroid.setRawPosition! 0 @19:50:35.04 Toroid.seek :home @19:50:35.16 Toroid.in :default @19:50:37.89 PinPort.in :default @19:50:38.26 Toroid.seek :clear @19:50:38.46 PinPort.in :unlocking @19:50:38.55 PinPort.seek :clear @19:50:41.92 Air.seek :clear @19:50:42.05 Lysis1.seek :clear @19:50:42.80 Lysis2.seek :clear @19:50:43.62 Archive.seek :clear @19:50:44.44 Carousel.in :default @19:50:44.60 Carousel.home.seek 46 @19:50:44.91 Selecting Cartridge 44 @19:50:45.26 Carousel.seek 44 @19:50:46.06 Carousel.reconfigure :align @19:50:46.22 Lysis1.in :fast @19:50:46.30 Lysis1.seek :locked @19:50:48.96 Plunge.seek :locked @19:50:49.17 Air.in :fast @19:50:49.25 Air.seek :locked @19:50:50.35 Carousel.reconfigure :locked @19:50:50.54 Powering cartridge bus @19:50:53.21 Gate.setIR 1750,5000,0,6 @19:50:53.34 Gate.setIR 0,5000,0,6 @19:50:53.43 PuckHeater.configure :default @19:50:53.53 Gate.power :heat,:ON,limit to 5000mA @19:50:53.72 Mounted lysis Cartridge board#J59915 @19:50:53.79 Using default cartridge heat loss curve for HeatingFunctionInCarousel @19:50:55.43 SPR.command "read_sequences" @19:50:55.96 SPR.command "stats_on" @19:50:56.13 SPR.command "start 0 1" @19:50:56.84 SPR.start /var/log/esp/spr26aug0119h.spr.bz2 @19:50:59.35 Waiting for baseline SPR stabilization @19:50:59.36 Handoff.select :waste @19:50:59.91 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:51:06.55 Syringe tare is 51% of full scale at 24C (drifted -0%) @19:51:06.58 PinPort.in :locking @19:51:06.68 PinPort.seek :locked @19:51:07.54 Archive.in :fast @19:51:07.63 Lysis2.in :fast @19:51:07.72 Lysis1.seek 950uL @19:51:07.84 Archive.seek :empty @19:51:08.02 Lysis2.seek 950uL @19:51:16.38 delayUntil "20:00:59.30" @20:00:59.32 Lysis2.in :DAmix @20:00:59.48 Lysis1.in :DAmix @20:01:00.15 Lysis2.seek 700uL @20:01:00.21 Lysis1.seek 700uL @20:01:35.22 delay 5 seconds @20:01:40.24 Handoff.select SPR @20:01:40.76 SPR.command "start 2 14" @20:01:42.51 SPR stabilized @20:01:42.69 delay 20 seconds @20:02:02.72 SPR.command "start 2 0" @20:02:05.76 Waiting up to 11:00 for SPR to enter state 1 @20:04:03.43 SPR.command "hold_on" @20:04:04.81 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:04:05.52 Lysis2.seek 400uL @20:04:05.57 Lysis1.seek 400uL @20:04:47.65 delay 15 seconds @20:05:02.68 SPR.command "hold_off" @20:05:05.46 Waiting up to 1:00:00 for SPR to enter state 7 @20:21:21.03 SPRsummary:4.43RIU,268RIU,251RIU,350RIU after 1926s @20:35:38.08 delay 20 seconds @20:35:58.11 SPR.command "start 2 0" @20:36:01.18 Waiting up to 11:00 for SPR to enter state 1 @20:37:58.86 SPR.command "hold_on" @20:38:00.24 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:38:00.95 Lysis2.seek 100uL @20:38:01.05 Lysis1.seek 100uL @20:38:42.97 delay 15 seconds @20:38:57.99 SPR.command "hold_off" @20:39:00.89 Waiting up to 1:00:00 for SPR to enter state 7 @21:09:33.39 SPR analysis completed @21:09:34.11 Ended log spr26aug0119h.spr.bz2 @21:09:34.13 SPR.command "stats_off" @21:09:35.12 Handoff.select :close @21:09:35.66 Gate.power :heat,:OFF @21:09:35.68 Gate.power :cartridge,:OFF @21:09:35.84 PinPort.in :unlocking @21:09:35.92 PinPort.seek :clear @21:09:36.77 Carousel.in :default @21:09:36.93 Carousel.home.seek 44 @21:09:37.29 Plunge.seek :locked @21:09:37.49 Archive.in :unlocking @21:09:37.66 Archive.seek :clear @21:09:37.85 Air.in :unlocking @21:09:38.01 Air.seek :clear @21:09:38.08 Lysis1.in :unlocking @21:09:38.32 Lysis1.seek :clear @21:09:38.45 Lysis2.in :unlocking @21:09:38.54 Lysis2.seek :clear @21:09:43.17 Gate.power :motor,:OFF @21:09:43.20 Gate.power :analytic1,:OFF @21:09:43.24 Gate.power :core,:OFF @21:09:43.28 Gate.power :main,:OFF @21:09:43.32 Safely stopped and ready to power off @21:09:43.53 Gate.power :sampler,:OFF,limit to 3500mA @21:09:43.56 Gate.power :skimmer,:OFF,limit to 3500mA @21:09:47.08 Gate.power :main,:ON,limit to 5000mA @21:09:47.11 Gate.power :analytic1,:ON,limit to 4000mA @21:09:47.22 Powering core bus @21:09:49.59 SPR Module 1, FW Rev. B, FPGA Rev. C @21:09:50.04 Plunge.configure :default @21:09:50.16 SamplePump.configure :default @21:09:50.27 Toroid.configure :default @21:09:50.38 Carousel.configure :default @21:09:50.49 Abs.configure :default @21:09:50.59 PhaseSeparator.configure :default @21:09:50.71 Archive.configure :default @21:09:50.81 Air.configure :default @21:09:50.89 Lysis1.configure :default @21:09:50.98 Lysis2.configure :default @21:09:51.09 PinPort.configure :default @21:09:51.17 All elf controllers running firmware version 4.94 @21:09:51.20 Gate.power :motor,:ON,limit to 5000mA @21:09:51.55 Plunge.home.jog 0 @21:09:52.31 Lysis2.home.jog 0 @21:09:52.36 Air.home.jog 0 @21:09:52.41 Carousel.home.jog 0 @21:09:52.46 Toroid.home.jog 0 @21:09:52.50 Lysis1.home.jog 0 @21:09:52.54 Archive.home.jog 0 @21:09:52.60 PinPort.home.jog 0 @21:09:53.44 Plunge.in :homing @21:09:53.73 Plunge.jog 1487 @21:09:53.95 Lysis2.in :homing @21:09:54.07 PinPort.in :homing @21:09:54.12 Lysis1.in :homing @21:09:54.16 Toroid.in :homing @21:09:54.21 Archive.in :homing @21:09:54.26 Air.in :homing @21:09:54.50 PinPort.jog 1238 @21:09:54.56 Lysis2.jog 1619 @21:09:54.78 Lysis1.jog 1603 @21:09:54.86 Air.jog 1432 @21:09:54.94 Toroid.jog 1355 @21:09:55.01 Archive.jog 1619 @21:09:55.38 Plunge.setRawPosition! 50 @21:09:55.46 Plunge.seek :home @21:09:55.79 Plunge.in :default @21:09:56.06 Lysis2.setRawPosition! 50 @21:09:56.15 Lysis2.seek :home @21:09:56.34 Air.setRawPosition! 0 @21:09:56.43 Air.seek :home @21:09:56.62 PinPort.setRawPosition! 3150 @21:09:56.67 Lysis2.in :default @21:09:56.70 Air.in :default @21:09:56.78 Lysis1.setRawPosition! 50 @21:09:56.84 Archive.setRawPosition! 50 @21:09:56.98 PinPort.seek :home @21:09:57.17 Archive.seek :home @21:09:57.23 Lysis1.seek :home @21:09:57.92 Archive.in :default @21:09:58.17 Lysis1.in :default @21:09:59.71 Toroid.setRawPosition! 0 @21:09:59.83 Toroid.seek :home @21:09:59.94 Toroid.in :default @21:10:01.52 PinPort.in :default @21:10:01.89 Toroid.seek :clear @21:10:02.07 PinPort.in :unlocking @21:10:02.16 PinPort.seek :clear @21:10:05.58 Air.seek :clear @21:10:05.71 Lysis1.seek :clear @21:10:06.47 Lysis2.seek :clear @21:10:07.29 Archive.seek :clear @21:10:08.10 Carousel.in :default @21:10:08.26 Carousel.home.seek 44 @21:10:08.55 Selecting Cartridge 26 @21:10:08.89 Carousel.seek 26 @21:10:12.93 Carousel.reconfigure :align @21:10:13.09 Lysis1.in :fast @21:10:13.17 Lysis1.seek :locked @21:10:15.87 Plunge.seek :locked @21:10:16.06 Air.in :fast @21:10:16.14 Air.seek :locked @21:10:17.24 Carousel.reconfigure :locked @21:10:17.42 Powering cartridge bus @21:10:20.09 Gate.setIR 1750,5000,0,6 @21:10:20.23 Gate.setIR 0,5000,0,6 @21:10:20.31 PuckHeater.configure :default @21:10:20.42 Gate.power :heat,:ON,limit to 5000mA @21:10:20.67 Mounted lysis Cartridge board#J59943 @21:10:20.73 Using default cartridge heat loss curve for HeatingFunctionInCarousel @21:10:22.32 SPR.command "read_sequences" @21:10:22.57 SPR.command "stats_on" @21:10:22.74 SPR.command "start 0 1" @21:10:23.45 SPR.start /var/log/esp/spr26aug0121h.spr.bz2 @21:10:25.42 Waiting for baseline SPR stabilization @21:10:25.44 Handoff.select :waste @21:10:26.00 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:10:33.12 Syringe tare is 51% of full scale at 24C (drifted -0%) @21:10:33.16 PinPort.in :locking @21:10:33.27 PinPort.seek :locked @21:10:34.14 Archive.in :fast @21:10:34.22 Lysis2.in :fast @21:10:34.31 Lysis1.seek 950uL @21:10:34.38 Archive.seek :empty @21:10:34.56 Lysis2.seek 950uL @21:10:43.00 delayUntil "21:20:24.99" @21:20:25.01 Lysis2.in :DAmix @21:20:25.16 Lysis1.in :DAmix @21:20:25.86 Lysis2.seek 700uL @21:20:25.92 Lysis1.seek 700uL @21:21:00.88 delay 5 seconds @21:21:05.91 Handoff.select SPR @21:21:06.42 SPR.command "start 2 14" @21:21:07.64 SPR stabilized @21:21:07.87 delay 20 seconds @21:21:27.89 SPR.command "start 2 0" @21:21:31.00 Waiting up to 11:00 for SPR to enter state 1 @21:23:29.08 SPR.command "hold_on" @21:23:30.47 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:23:31.15 Lysis2.seek 400uL @21:23:31.21 Lysis1.seek 400uL @21:24:13.19 delay 15 seconds @21:24:28.21 SPR.command "hold_off" @21:24:31.12 Waiting up to 1:00:00 for SPR to enter state 7 @21:40:46.70 SPRsummary:3.02RIU,240RIU,224RIU,306RIU after 1925s @21:55:03.74 delay 20 seconds @21:55:23.76 SPR.command "start 2 0" @21:55:26.85 Waiting up to 11:00 for SPR to enter state 1 @21:57:24.52 SPR.command "hold_on" @21:57:25.90 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:57:26.64 Lysis2.seek 100uL @21:57:26.71 Lysis1.seek 100uL @21:58:08.65 delay 15 seconds @21:58:23.68 SPR.command "hold_off" @21:58:27.09 Waiting up to 1:00:00 for SPR to enter state 7 @22:28:59.98 SPR analysis completed @22:29:00.64 Ended log spr26aug0121h.spr.bz2 @22:29:00.66 SPR.command "stats_off" @22:29:01.84 Handoff.select :close @22:29:02.38 Gate.power :heat,:OFF @22:29:02.41 Gate.power :cartridge,:OFF @22:29:02.53 PinPort.in :unlocking @22:29:02.61 PinPort.seek :clear @22:29:03.47 Carousel.in :default @22:29:03.63 Carousel.home.seek 26 @22:29:04.00 Plunge.seek :locked @22:29:04.22 Archive.in :unlocking @22:29:04.37 Archive.seek :clear @22:29:04.50 Air.in :unlocking @22:29:04.68 Air.seek :clear @22:29:04.76 Lysis1.in :unlocking @22:29:04.97 Lysis1.seek :clear @22:29:05.10 Lysis2.in :unlocking @22:29:05.19 Lysis2.seek :clear @22:29:09.85 Gate.power :motor,:OFF @22:29:09.90 Gate.power :analytic1,:OFF @22:29:09.94 Gate.power :core,:OFF @22:29:09.97 Gate.power :main,:OFF @22:29:10.01 Safely stopped and ready to power off @22:29:10.33 Gate.power :sampler,:OFF,limit to 3500mA @22:29:10.36 Gate.power :skimmer,:OFF,limit to 3500mA @22:29:13.80 Gate.power :main,:ON,limit to 5000mA @22:29:13.84 Gate.power :analytic1,:ON,limit to 4000mA @22:29:13.96 Powering core bus @22:29:16.30 SPR Module 1, FW Rev. B, FPGA Rev. C @22:29:16.77 Plunge.configure :default @22:29:16.88 SamplePump.configure :default @22:29:17.00 Toroid.configure :default @22:29:17.10 Carousel.configure :default @22:29:17.20 Abs.configure :default @22:29:17.32 PhaseSeparator.configure :default @22:29:17.43 Archive.configure :default @22:29:17.55 Air.configure :default @22:29:17.63 Lysis1.configure :default @22:29:17.71 Lysis2.configure :default @22:29:17.85 PinPort.configure :default @22:29:17.93 All elf controllers running firmware version 4.94 @22:29:17.96 Gate.power :motor,:ON,limit to 5000mA @22:29:18.21 Plunge.home.jog 0 @22:29:19.03 PinPort.home.jog 0 @22:29:19.09 Lysis1.home.jog 0 @22:29:19.14 Archive.home.jog 0 @22:29:19.19 Toroid.home.jog 0 @22:29:19.23 Lysis2.home.jog 0 @22:29:19.29 Air.home.jog 0 @22:29:19.33 Carousel.home.jog 0 @22:29:20.18 Plunge.in :homing @22:29:20.48 Plunge.jog 1483 @22:29:20.69 Archive.in :homing @22:29:20.80 Lysis1.in :homing @22:29:20.87 Lysis2.in :homing @22:29:20.93 Air.in :homing @22:29:20.97 Toroid.in :homing @22:29:21.03 PinPort.in :homing @22:29:21.23 Lysis1.jog 1604 @22:29:21.41 Archive.jog 1621 @22:29:21.46 Air.jog 1431 @22:29:21.51 Lysis2.jog 1620 @22:29:21.57 Toroid.jog 1358 @22:29:21.66 PinPort.jog 1251 @22:29:22.15 Plunge.setRawPosition! 50 @22:29:22.23 Plunge.seek :home @22:29:22.54 Plunge.in :default @22:29:22.95 Lysis1.setRawPosition! 50 @22:29:23.08 Air.setRawPosition! 0 @22:29:23.11 Lysis1.seek :home @22:29:23.27 Air.seek :home @22:29:23.95 PinPort.setRawPosition! 3150 @22:29:24.10 Lysis2.setRawPosition! 50 @22:29:24.13 Lysis1.in :default @22:29:24.17 Archive.setRawPosition! 50 @22:29:24.22 Air.in :default @22:29:24.49 PinPort.seek :home @22:29:24.53 Lysis2.seek :home @22:29:24.60 Archive.seek :home @22:29:24.99 Lysis2.in :default @22:29:25.11 Archive.in :default @22:29:26.43 Toroid.setRawPosition! 0 @22:29:26.51 Toroid.seek :home @22:29:26.63 Toroid.in :default @22:29:29.04 PinPort.in :default @22:29:29.41 Toroid.seek :clear @22:29:29.61 PinPort.in :unlocking @22:29:29.71 PinPort.seek :clear @22:29:33.11 Air.seek :clear @22:29:33.25 Lysis1.seek :clear @22:29:33.99 Lysis2.seek :clear @22:29:34.82 Archive.seek :clear @22:29:35.63 Carousel.in :default @22:29:35.82 Carousel.home.seek 26 @22:29:36.13 Selecting Cartridge 24 @22:29:36.47 Carousel.seek 24 @22:29:37.26 Carousel.reconfigure :align @22:29:37.42 Lysis1.in :fast @22:29:37.50 Lysis1.seek :locked @22:29:40.17 Plunge.seek :locked @22:29:40.36 Air.in :fast @22:29:40.45 Air.seek :locked @22:29:41.54 Carousel.reconfigure :locked @22:29:41.72 Powering cartridge bus @22:29:44.39 Gate.setIR 1750,5000,0,6 @22:29:44.53 Gate.setIR 0,5000,0,6 @22:29:44.62 PuckHeater.configure :default @22:29:44.72 Gate.power :heat,:ON,limit to 5000mA @22:29:44.93 Mounted lysis Cartridge board#J59986 @22:29:44.98 Using default cartridge heat loss curve for HeatingFunctionInCarousel @22:29:46.70 SPR.command "read_sequences" @22:29:47.41 SPR.command "stats_on" @22:29:47.58 SPR.command "start 0 1" @22:29:48.29 SPR.start /var/log/esp/spr26aug0122h.spr.bz2 @22:29:50.79 Waiting for baseline SPR stabilization @22:29:50.81 Handoff.select :waste @22:29:51.35 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:29:57.99 Syringe tare is 51% of full scale at 23C (drifted -0%) @22:29:58.04 PinPort.in :locking @22:29:58.12 PinPort.seek :locked @22:29:58.99 Archive.in :fast @22:29:59.08 Lysis2.in :fast @22:29:59.17 Lysis1.seek 950uL @22:29:59.27 Archive.seek :empty @22:29:59.44 Lysis2.seek 950uL @22:30:07.88 delayUntil "22:39:50.75" @22:39:50.77 Lysis2.in :DAmix @22:39:50.92 Lysis1.in :DAmix @22:39:51.58 Lysis2.seek 700uL @22:39:51.64 Lysis1.seek 700uL @22:40:26.72 delay 5 seconds @22:40:31.75 Handoff.select SPR @22:40:32.32 SPR.command "start 2 14" @22:40:33.43 SPR stabilized @22:40:33.61 delay 20 seconds @22:40:53.64 SPR.command "start 2 0" @22:40:56.26 Waiting up to 11:00 for SPR to enter state 1 @22:42:53.93 SPR.command "hold_on" @22:42:55.31 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:42:56.06 Lysis2.seek 400uL @22:42:56.10 Lysis1.seek 400uL @22:43:38.09 delay 15 seconds @22:43:53.11 SPR.command "hold_off" @22:43:55.96 Waiting up to 1:00:00 for SPR to enter state 7 @23:00:11.54 SPRsummary:3.63RIU,186RIU,167RIU,239RIU after 1925s @23:14:28.58 delay 20 seconds @23:14:48.61 SPR.command "start 2 0" @23:14:52.26 Waiting up to 11:00 for SPR to enter state 1 @23:16:50.31 SPR.command "hold_on" @23:16:51.69 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:16:52.83 Lysis2.seek 100uL @23:16:52.92 Lysis1.seek 100uL @23:17:34.86 delay 15 seconds @23:17:49.90 SPR.command "hold_off" @23:17:53.28 Waiting up to 1:00:00 for SPR to enter state 7 @23:48:25.77 SPR analysis completed @23:48:26.53 Ended log spr26aug0122h.spr.bz2 @23:48:26.55 SPR.command "stats_off" @23:48:27.43 Handoff.select :close @23:48:27.97 Gate.power :heat,:OFF @23:48:27.99 Gate.power :cartridge,:OFF @23:48:28.12 PinPort.in :unlocking @23:48:28.20 PinPort.seek :clear @23:48:29.05 Carousel.in :default @23:48:29.21 Carousel.home.seek 24 @23:48:29.59 Plunge.seek :locked @23:48:29.85 Archive.in :unlocking @23:48:30.01 Archive.seek :clear @23:48:30.15 Air.in :unlocking @23:48:30.30 Air.seek :clear @23:48:30.39 Lysis1.in :unlocking @23:48:31.12 Lysis1.seek :clear @23:48:31.27 Lysis2.in :unlocking @23:48:31.36 Lysis2.seek :clear @23:48:36.01 Gate.power :motor,:OFF @23:48:36.03 Gate.power :analytic1,:OFF @23:48:36.06 Gate.power :core,:OFF @23:48:36.11 Gate.power :main,:OFF @23:48:36.15 Safely stopped and ready to power off @23:48:36.82 Gate.power :sampler,:OFF,limit to 3500mA @23:48:36.84 Gate.power :skimmer,:OFF,limit to 3500mA @23:48:39.92 Gate.power :main,:ON,limit to 5000mA @23:48:39.96 Gate.power :analytic1,:ON,limit to 4000mA @23:48:40.07 Powering core bus @23:48:42.42 SPR Module 1, FW Rev. B, FPGA Rev. C @23:48:42.86 Plunge.configure :default @23:48:42.98 SamplePump.configure :default @23:48:43.08 Toroid.configure :default @23:48:43.20 Carousel.configure :default @23:48:43.32 Abs.configure :default @23:48:43.43 PhaseSeparator.configure :default @23:48:43.54 Archive.configure :default @23:48:43.65 Air.configure :default @23:48:43.72 Lysis1.configure :default @23:48:43.85 Lysis2.configure :default @23:48:43.97 PinPort.configure :default @23:48:44.06 All elf controllers running firmware version 4.94 @23:48:44.09 Gate.power :motor,:ON,limit to 5000mA @23:48:44.86 Lysis1.home.jog 0 @23:48:44.98 Air.home.jog 0 @23:48:45.02 Carousel.home.jog 0 @23:48:45.08 Lysis2.home.jog 0 @23:48:45.13 Archive.home.jog 0 @23:48:45.19 Plunge.home.jog 0 @23:48:45.24 Toroid.home.jog 0 @23:48:46.05 PinPort.home.jog 0 @23:48:46.62 Lysis1.in :homing @23:48:46.72 Air.in :homing @23:48:46.78 Archive.in :homing @23:48:46.83 Plunge.in :homing @23:48:46.87 Lysis2.in :homing @23:48:46.92 Toroid.in :homing @23:48:47.20 Lysis1.jog 1604 @23:48:47.27 Air.jog 1431 @23:48:47.31 Archive.jog 1618 @23:48:47.36 Lysis2.jog 1619 @23:48:47.40 Toroid.jog 1348 @23:48:47.45 Plunge.jog 1483 @23:48:47.96 PinPort.in :homing @23:48:48.04 PinPort.jog 1248 @23:48:48.75 Air.setRawPosition! 0 @23:48:48.83 Air.seek :home @23:48:49.48 Lysis1.setRawPosition! 50 @23:48:49.62 Air.in :default @23:48:49.67 Plunge.setRawPosition! 50 @23:48:49.71 Lysis2.setRawPosition! 50 @23:48:49.75 Archive.setRawPosition! 50 @23:48:50.03 Lysis1.seek :home @23:48:50.13 Plunge.seek :home @23:48:50.19 Lysis2.seek :home @23:48:50.24 Archive.seek :home @23:48:50.58 PinPort.setRawPosition! 3150 @23:48:50.67 PinPort.seek :home @23:48:50.87 Lysis2.in :default @23:48:50.89 Lysis1.in :default @23:48:50.94 Plunge.in :default @23:48:50.98 Archive.in :default @23:48:52.28 Toroid.setRawPosition! 0 @23:48:52.37 Toroid.seek :home @23:48:52.49 Toroid.in :default @23:48:55.08 PinPort.in :default @23:48:55.45 Toroid.seek :clear @23:48:55.64 PinPort.in :unlocking @23:48:55.73 PinPort.seek :clear @23:48:59.15 Air.seek :clear @23:48:59.29 Lysis1.seek :clear @23:49:00.04 Lysis2.seek :clear @23:49:00.86 Archive.seek :clear @23:49:01.68 Carousel.in :default @23:49:01.88 Carousel.home.seek 24 @23:49:02.19 Selecting Cartridge 22 @23:49:02.53 Carousel.seek 22 @23:49:03.31 Carousel.reconfigure :align @23:49:03.47 Lysis1.in :fast @23:49:03.58 Slide::Warning in core -- Storage Carousel (SC) reseek at 22 + 5 counts @23:49:03.63 Lysis1.seek :locked @23:49:06.29 Plunge.seek :locked @23:49:06.51 Air.in :fast @23:49:06.59 Air.seek :locked @23:49:07.68 Carousel.reconfigure :locked @23:49:07.90 Powering cartridge bus @23:49:10.58 Gate.setIR 1750,5000,0,6 @23:49:10.71 Gate.setIR 0,5000,0,6 @23:49:10.80 PuckHeater.configure :default @23:49:10.90 Gate.power :heat,:ON,limit to 5000mA @23:49:11.10 Mounted lysis Cartridge board#J59951 @23:49:11.16 Using default cartridge heat loss curve for HeatingFunctionInCarousel @23:49:13.34 SPR.command "read_sequences" @23:49:13.52 SPR.command "stats_on" @23:49:13.71 SPR.command "start 0 1" @23:49:14.41 SPR.start /var/log/esp/spr26aug0123h.spr.bz2 @23:49:16.38 Waiting for baseline SPR stabilization @23:49:16.40 Handoff.select :waste @23:49:16.94 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @23:49:23.64 Syringe tare is 51% of full scale at 24C (drifted -0%) @23:49:23.67 PinPort.in :locking @23:49:23.76 PinPort.seek :locked @23:49:24.68 Archive.in :fast @23:49:24.76 Lysis2.in :fast @23:49:24.85 Lysis1.seek 950uL @23:49:24.99 Archive.seek :empty @23:49:25.10 Lysis2.seek 950uL @23:49:33.53 delayUntil "23:59:15.95" @23:59:15.97 Lysis2.in :DAmix @23:59:16.11 Lysis1.in :DAmix @23:59:16.80 Lysis2.seek 700uL @23:59:16.84 Lysis1.seek 700uL @23:59:51.87 delay 5 seconds @23:59:56.89 Handoff.select SPR @23:59:57.42 SPR.command "start 2 14" @23:59:59.12 SPR stabilized @23:59:59.31 delay 20 seconds @00:00:19.33EDT02-Aug-26 SPR.command "start 2 0" @00:00:22.91 Waiting up to 11:00 for SPR to enter state 1 @00:02:20.99 SPR.command "hold_on" @00:02:22.91 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:02:23.59 Lysis2.seek 400uL @00:02:23.69 Lysis1.seek 400uL @00:03:05.68 delay 15 seconds @00:03:20.70 SPR.command "hold_off" @00:03:24.49 Waiting up to 1:00:00 for SPR to enter state 7 @00:19:39.54 SPRsummary:1.28RIU,97.1RIU,85.5RIU,117RIU after 1927s @00:33:56.59 delay 20 seconds @00:34:16.61 SPR.command "start 2 0" @00:34:19.70 Waiting up to 11:00 for SPR to enter state 1 @00:36:17.37 SPR.command "hold_on" @00:36:19.27 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:36:20.06 Lysis2.seek 100uL @00:36:20.10 Lysis1.seek 100uL @00:37:02.12 delay 15 seconds @00:37:17.14 SPR.command "hold_off" @00:37:20.87 Waiting up to 1:00:00 for SPR to enter state 7 @01:07:52.83 SPR analysis completed @01:07:53.50 Ended log spr26aug0123h.spr.bz2 @01:07:53.52 SPR.command "stats_off" @01:07:54.63 Handoff.select :close @01:07:55.17 Gate.power :heat,:OFF @01:07:55.19 Gate.power :cartridge,:OFF @01:07:55.32 PinPort.in :unlocking @01:07:55.41 PinPort.seek :clear @01:07:56.26 Carousel.in :default @01:07:56.42 Carousel.home.seek 22 @01:07:56.78 Plunge.seek :locked @01:07:57.00 Archive.in :unlocking @01:07:57.15 Archive.seek :clear @01:07:57.30 Air.in :unlocking @01:07:57.46 Air.seek :clear @01:07:57.60 Lysis1.in :unlocking @01:07:58.31 Lysis1.seek :clear @01:07:58.46 Lysis2.in :unlocking @01:07:58.59 Lysis2.seek :clear @01:08:03.23 Gate.power :motor,:OFF @01:08:03.26 Gate.power :analytic1,:OFF @01:08:03.30 Gate.power :core,:OFF @01:08:03.34 Gate.power :main,:OFF @01:08:03.37 Safely stopped and ready to power off @01:08:03.61 Gate.power :sampler,:OFF,limit to 3500mA @01:08:03.63 Gate.power :skimmer,:OFF,limit to 3500mA @01:08:07.14 Gate.power :main,:ON,limit to 5000mA @01:08:07.17 Gate.power :analytic1,:ON,limit to 4000mA @01:08:07.29 Powering core bus @01:08:09.63 SPR Module 1, FW Rev. B, FPGA Rev. C @01:08:10.09 Plunge.configure :default @01:08:10.21 SamplePump.configure :default @01:08:10.32 Toroid.configure :default @01:08:10.43 Carousel.configure :default @01:08:10.55 Abs.configure :default @01:08:10.65 PhaseSeparator.configure :default @01:08:10.76 Archive.configure :default @01:08:10.87 Air.configure :default @01:08:10.95 Lysis1.configure :default @01:08:11.04 Lysis2.configure :default @01:08:11.15 PinPort.configure :default @01:08:11.23 All elf controllers running firmware version 4.94 @01:08:11.26 Gate.power :motor,:ON,limit to 5000mA @01:08:11.63 Plunge.home.jog 0 @01:08:12.35 PinPort.home.jog 0 @01:08:12.42 Lysis1.home.jog 0 @01:08:12.46 Archive.home.jog 0 @01:08:12.53 Toroid.home.jog 0 @01:08:12.58 Lysis2.home.jog 0 @01:08:12.61 Air.home.jog 0 @01:08:12.65 Carousel.home.jog 0 @01:08:13.47 Plunge.in :homing @01:08:13.92 Plunge.jog 1487 @01:08:14.01 Lysis2.in :homing @01:08:14.08 Air.in :homing @01:08:14.12 Toroid.in :homing @01:08:14.17 PinPort.in :homing @01:08:14.21 Archive.in :homing @01:08:14.26 Lysis1.in :homing @01:08:14.56 Lysis2.jog 1619 @01:08:14.76 Air.jog 1433 @01:08:14.83 Toroid.jog 1349 @01:08:14.87 Lysis1.jog 1604 @01:08:14.94 PinPort.jog 1250 @01:08:14.99 Archive.jog 1618 @01:08:15.57 Plunge.setRawPosition! 50 @01:08:15.65 Plunge.seek :home @01:08:16.45 Plunge.in :default @01:08:16.67 Lysis1.setRawPosition! 50 @01:08:16.73 Air.setRawPosition! 0 @01:08:16.77 Lysis2.setRawPosition! 50 @01:08:16.83 Archive.setRawPosition! 50 @01:08:17.04 Lysis1.seek :home @01:08:17.09 Air.seek :home @01:08:17.13 Lysis2.seek :home @01:08:17.19 Archive.seek :home @01:08:17.56 Air.in :default @01:08:17.60 PinPort.setRawPosition! 3150 @01:08:17.74 PinPort.seek :home @01:08:17.95 Lysis1.in :default @01:08:17.99 Archive.in :default @01:08:18.06 Lysis2.in :default @01:08:19.59 Toroid.setRawPosition! 0 @01:08:19.68 Toroid.seek :home @01:08:19.83 Toroid.in :default @01:08:22.18 PinPort.in :default @01:08:22.55 Toroid.seek :clear @01:08:22.75 PinPort.in :unlocking @01:08:22.84 PinPort.seek :clear @01:08:26.25 Air.seek :clear @01:08:26.40 Lysis1.seek :clear @01:08:27.15 Lysis2.seek :clear @01:08:27.97 Archive.seek :clear @01:08:28.80 Carousel.in :default @01:08:28.95 Carousel.home.seek 22 @01:08:29.24 Selecting Cartridge 20 @01:08:29.60 Carousel.seek 20 @01:08:30.39 Carousel.reconfigure :align @01:08:30.54 Lysis1.in :fast @01:08:30.63 Lysis1.seek :locked @01:08:33.29 Plunge.seek :locked @01:08:33.49 Air.in :fast @01:08:33.57 Air.seek :locked @01:08:34.67 Carousel.reconfigure :locked @01:08:34.85 Powering cartridge bus @01:08:37.53 Gate.setIR 1750,5000,0,6 @01:08:37.66 Gate.setIR 0,5000,0,6 @01:08:37.75 PuckHeater.configure :default @01:08:37.89 Gate.power :heat,:ON,limit to 5000mA @01:08:38.08 Mounted lysis Cartridge board#J59971 @01:08:38.19 Using default cartridge heat loss curve for HeatingFunctionInCarousel @01:08:40.41 SPR.command "read_sequences" @01:08:40.74 SPR.command "stats_on" @01:08:40.93 SPR.command "start 0 1" @01:08:41.63 SPR.start /var/log/esp/spr26aug0201h.spr.bz2 @01:08:44.12 Waiting for baseline SPR stabilization @01:08:44.14 Handoff.select :waste @01:08:44.69 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:08:51.32 Syringe tare is 51% of full scale at 23C (drifted -0%) @01:08:51.36 PinPort.in :locking @01:08:51.47 PinPort.seek :locked @01:08:52.33 Archive.in :fast @01:08:52.42 Lysis2.in :fast @01:08:52.53 Archive.seek :empty @01:08:52.64 Lysis1.seek 950uL @01:08:52.69 Lysis2.seek 950uL @01:09:01.13 delayUntil "01:18:44.08" @01:18:44.10 Lysis2.in :DAmix @01:18:44.25 Lysis1.in :DAmix @01:18:44.94 Lysis2.seek 700uL @01:18:44.98 Lysis1.seek 700uL @01:19:19.99 delay 5 seconds @01:19:25.02 Handoff.select SPR @01:19:25.55 SPR.command "start 2 14" @01:19:26.76 SPR stabilized @01:19:26.97 delay 20 seconds @01:19:46.99 SPR.command "start 2 0" @01:19:50.12 Waiting up to 11:00 for SPR to enter state 1 @01:21:48.20 SPR.command "hold_on" @01:21:49.59 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:21:50.36 Lysis2.seek 400uL @01:21:50.39 Lysis1.seek 400uL @01:22:32.37 delay 15 seconds @01:22:47.39 SPR.command "hold_off" @01:22:50.77 Waiting up to 1:00:00 for SPR to enter state 7 @01:39:06.76 SPRsummary:2.64RIU,27.5RIU,14.2RIU,28.2RIU after 1927s @01:53:23.84 delay 20 seconds @01:53:43.86 SPR.command "start 2 0" @01:53:46.92 Waiting up to 11:00 for SPR to enter state 1 @01:55:44.60 SPR.command "hold_on" @01:55:46.49 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:55:47.22 Lysis2.seek 100uL @01:55:47.27 Lysis1.seek 100uL @01:56:29.28 delay 15 seconds @01:56:44.30 SPR.command "hold_off" @01:56:47.56 Waiting up to 1:00:00 for SPR to enter state 7 @02:27:19.11 SPR analysis completed @02:27:19.77 Ended log spr26aug0201h.spr.bz2 @02:27:19.82 SPR.command "stats_off" @02:27:20.86 Handoff.select :close @02:27:21.41 Gate.power :heat,:OFF @02:27:21.43 Gate.power :cartridge,:OFF @02:27:21.57 PinPort.in :unlocking @02:27:21.65 PinPort.seek :clear @02:27:22.49 Carousel.in :default @02:27:22.64 Carousel.home.seek 20 @02:27:23.03 Plunge.seek :locked @02:27:23.28 Archive.in :unlocking @02:27:23.39 Archive.seek :clear @02:27:23.52 Air.in :unlocking @02:27:23.67 Air.seek :clear @02:27:23.83 Lysis1.in :unlocking @02:27:24.00 Lysis1.seek :clear @02:27:24.14 Lysis2.in :unlocking @02:27:24.22 Lysis2.seek :clear @02:27:28.86 Gate.power :motor,:OFF @02:27:29.39 Gate.power :analytic1,:OFF @02:27:29.42 Gate.power :core,:OFF @02:27:29.46 Gate.power :main,:OFF @02:27:29.51 Safely stopped and ready to power off @02:27:29.76 Gate.power :sampler,:OFF,limit to 3500mA @02:27:29.79 Gate.power :skimmer,:OFF,limit to 3500mA @02:27:33.27 Gate.power :main,:ON,limit to 5000mA @02:27:33.30 Gate.power :analytic1,:ON,limit to 4000mA @02:27:33.43 Powering core bus @02:27:35.79 SPR Module 1, FW Rev. B, FPGA Rev. C @02:27:36.25 Plunge.configure :default @02:27:36.36 SamplePump.configure :default @02:27:36.47 Toroid.configure :default @02:27:36.58 Carousel.configure :default @02:27:36.69 Abs.configure :default @02:27:36.80 PhaseSeparator.configure :default @02:27:36.91 Archive.configure :default @02:27:37.01 Air.configure :default @02:27:37.09 Lysis1.configure :default @02:27:37.16 Lysis2.configure :default @02:27:37.27 PinPort.configure :default @02:27:37.36 All elf controllers running firmware version 4.94 @02:27:37.40 Gate.power :motor,:ON,limit to 5000mA @02:27:37.87 Plunge.home.jog 0 @02:27:38.54 PinPort.home.jog 0 @02:27:38.59 Lysis1.home.jog 0 @02:27:38.63 Carousel.home.jog 0 @02:27:38.68 Toroid.home.jog 0 @02:27:38.73 Air.home.jog 0 @02:27:38.78 Archive.home.jog 0 @02:27:38.84 Lysis2.home.jog 0 @02:27:39.65 Plunge.in :homing @02:27:40.05 Plunge.jog 1492 @02:27:40.26 PinPort.in :homing @02:27:40.34 Lysis2.in :homing @02:27:40.39 Air.in :homing @02:27:40.44 Toroid.in :homing @02:27:40.48 Lysis1.in :homing @02:27:40.56 Archive.in :homing @02:27:40.78 PinPort.jog 1242 @02:27:40.89 Lysis2.jog 1617 @02:27:40.96 Lysis1.jog 1601 @02:27:41.07 Air.jog 1432 @02:27:41.11 Toroid.jog 1354 @02:27:41.20 Archive.jog 1620 @02:27:41.60 Plunge.setRawPosition! 50 @02:27:41.68 Plunge.seek :home @02:27:42.00 Plunge.in :default @02:27:42.53 Lysis1.setRawPosition! 50 @02:27:42.65 Lysis2.setRawPosition! 50 @02:27:42.71 Air.setRawPosition! 0 @02:27:42.78 Lysis1.seek :home @02:27:42.89 Lysis2.seek :home @02:27:42.96 Air.seek :home @02:27:43.72 PinPort.setRawPosition! 3150 @02:27:43.91 Lysis2.in :default @02:27:43.94 Lysis1.in :default @02:27:43.99 Air.in :default @02:27:44.04 Archive.setRawPosition! 50 @02:27:44.25 PinPort.seek :home @02:27:44.42 Archive.seek :home @02:27:44.76 Archive.in :default @02:27:45.90 Toroid.setRawPosition! 0 @02:27:45.98 Toroid.seek :home @02:27:46.10 Toroid.in :default @02:27:48.73 PinPort.in :default @02:27:49.10 Toroid.seek :clear @02:27:49.30 PinPort.in :unlocking @02:27:49.39 PinPort.seek :clear @02:27:52.75 Air.seek :clear @02:27:52.88 Lysis1.seek :clear @02:27:53.63 Lysis2.seek :clear @02:27:54.45 Archive.seek :clear @02:27:55.27 Carousel.in :default @02:27:55.43 Carousel.home.seek 20 @02:27:55.76 Selecting Cartridge 14 @02:27:56.15 Carousel.seek 14 @02:27:57.81 Carousel.reconfigure :align @02:27:57.98 Lysis1.in :fast @02:27:58.06 Lysis1.seek :locked @02:27:58.13 Slide::Warning in core -- Storage Carousel (SC) reseek at 14 + 4 counts @02:28:00.80 Plunge.seek :locked @02:28:01.01 Air.in :fast @02:28:01.09 Air.seek :locked @02:28:02.18 Carousel.reconfigure :locked @02:28:02.37 Powering cartridge bus @02:28:05.04 Gate.setIR 1750,5000,0,6 @02:28:05.17 Gate.setIR 0,5000,0,6 @02:28:05.26 PuckHeater.configure :default @02:28:05.36 Gate.power :heat,:ON,limit to 5000mA @02:28:05.54 Mounted lysis Cartridge board#J59913 @02:28:05.60 Using default cartridge heat loss curve for HeatingFunctionInCarousel @02:28:07.23 SPR.command "read_sequences" @02:28:07.83 SPR.command "stats_on" @02:28:08.87 SPR.command "start 0 1" @02:28:09.57 SPR.start /var/log/esp/spr26aug0202h.spr.bz2 @02:28:12.15 Waiting for baseline SPR stabilization @02:28:12.16 Handoff.select :waste @02:28:12.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:28:19.34 Syringe tare is 51% of full scale at 23C (drifted -0%) @02:28:19.37 PinPort.in :locking @02:28:19.48 PinPort.seek :locked @02:28:20.35 Archive.in :fast @02:28:20.46 Lysis2.in :fast @02:28:20.52 Lysis1.seek 950uL @02:28:20.63 Archive.seek :empty @02:28:20.78 Lysis2.seek 950uL @02:28:29.21 delayUntil "02:38:12.11" @02:38:12.12 Lysis2.in :DAmix @02:38:12.27 Lysis1.in :DAmix @02:38:12.96 Lysis2.seek 700uL @02:38:13.04 Lysis1.seek 700uL @02:38:48.03 delay 5 seconds @02:38:53.06 Handoff.select SPR @02:38:53.58 SPR.command "start 2 14" @02:38:54.79 SPR stabilized @02:38:54.97 delay 20 seconds @02:39:14.99 SPR.command "start 2 0" @02:39:17.61 Waiting up to 11:00 for SPR to enter state 1 @02:41:15.28 SPR.command "hold_on" @02:41:16.68 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @02:41:17.40 Lysis2.seek 400uL @02:41:17.54 Lysis1.seek 400uL @02:41:59.42 delay 15 seconds @02:42:14.44 SPR.command "hold_off" @02:42:17.32 Waiting up to 1:00:00 for SPR to enter state 7 @02:58:32.90 SPRsummary:2.61RIU,10.7RIU,-2.28RIU,9.27RIU after 1925s @03:12:49.99 delay 20 seconds @03:13:10.02 SPR.command "start 2 0" @03:13:13.05 Waiting up to 11:00 for SPR to enter state 1 @03:15:10.72 SPR.command "hold_on" @03:15:12.10 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @03:15:12.83 Lysis2.seek 100uL @03:15:12.89 Lysis1.seek 100uL @03:15:54.86 delay 15 seconds @03:16:09.88 SPR.command "hold_off" @03:16:12.75 Waiting up to 1:00:00 for SPR to enter state 7 @03:46:45.23 SPR analysis completed @03:46:45.89 Ended log spr26aug0202h.spr.bz2 @03:46:45.91 SPR.command "stats_off" @03:46:47.06 Handoff.select :close @03:46:47.60 Gate.power :heat,:OFF @03:46:47.63 Gate.power :cartridge,:OFF @03:46:47.75 PinPort.in :unlocking @03:46:47.86 PinPort.seek :clear @03:46:48.72 Carousel.in :default @03:46:48.88 Carousel.home.seek 14 @03:46:49.25 Plunge.seek :locked @03:46:49.47 Archive.in :unlocking @03:46:49.63 Archive.seek :clear @03:46:49.77 Air.in :unlocking @03:46:49.97 Air.seek :clear @03:46:50.05 Lysis1.in :unlocking @03:46:50.24 Lysis1.seek :clear @03:46:50.40 Lysis2.in :unlocking @03:46:50.55 Lysis2.seek :clear @03:46:55.24 Gate.power :motor,:OFF @03:46:55.26 Gate.power :analytic1,:OFF @03:46:55.31 Gate.power :core,:OFF @03:46:55.35 Gate.power :main,:OFF @03:46:55.38 Safely stopped and ready to power off @03:46:55.59 Gate.power :sampler,:OFF,limit to 3500mA @03:46:55.61 Gate.power :skimmer,:OFF,limit to 3500mA @03:46:59.15 Gate.power :main,:ON,limit to 5000mA @03:46:59.18 Gate.power :analytic1,:ON,limit to 4000mA @03:46:59.87 Powering core bus @03:47:01.63 SPR Module 1, FW Rev. B, FPGA Rev. C @03:47:02.68 Plunge.configure :default @03:47:02.79 SamplePump.configure :default @03:47:02.90 Toroid.configure :default @03:47:03.01 Carousel.configure :default @03:47:03.12 Abs.configure :default @03:47:03.22 PhaseSeparator.configure :default @03:47:03.33 Archive.configure :default @03:47:03.44 Air.configure :default @03:47:03.52 Lysis1.configure :default @03:47:03.60 Lysis2.configure :default @03:47:03.70 PinPort.configure :default @03:47:03.79 All elf controllers running firmware version 4.94 @03:47:03.84 Gate.power :motor,:ON,limit to 5000mA @03:47:04.18 Plunge.home.jog 0 @03:47:04.90 PinPort.home.jog 0 @03:47:04.97 Lysis1.home.jog 0 @03:47:05.02 Archive.home.jog 0 @03:47:05.06 Toroid.home.jog 0 @03:47:05.10 Lysis2.home.jog 0 @03:47:05.15 Air.home.jog 0 @03:47:05.21 Carousel.home.jog 0 @03:47:06.15 Plunge.in :homing @03:47:06.57 Plunge.jog 1486 @03:47:06.67 PinPort.in :homing @03:47:06.72 Lysis1.in :homing @03:47:06.77 Toroid.in :homing @03:47:06.81 Lysis2.in :homing @03:47:06.86 Air.in :homing @03:47:06.90 Archive.in :homing @03:47:07.17 PinPort.jog 1241 @03:47:07.22 Lysis1.jog 1602 @03:47:07.47 Air.jog 1432 @03:47:07.52 Toroid.jog 1351 @03:47:07.56 Lysis2.jog 1620 @03:47:07.64 Archive.jog 1622 @03:47:08.22 Plunge.setRawPosition! 50 @03:47:08.31 Plunge.seek :home @03:47:08.61 Plunge.in :default @03:47:08.73 Lysis1.setRawPosition! 50 @03:47:08.80 Lysis1.seek :home @03:47:08.93 Air.setRawPosition! 0 @03:47:09.00 Air.seek :home @03:47:09.18 Lysis2.setRawPosition! 50 @03:47:09.23 Lysis1.in :default @03:47:09.27 Archive.setRawPosition! 50 @03:47:09.31 Air.in :default @03:47:09.49 Lysis2.seek :home @03:47:09.67 Archive.seek :home @03:47:10.35 PinPort.setRawPosition! 3150 @03:47:10.47 Lysis2.in :default @03:47:10.62 PinPort.seek :home @03:47:10.76 Archive.in :default @03:47:12.31 Toroid.setRawPosition! 0 @03:47:12.41 Toroid.seek :home @03:47:12.51 Toroid.in :default @03:47:15.02 PinPort.in :default @03:47:15.39 Toroid.seek :clear @03:47:15.58 PinPort.in :unlocking @03:47:15.66 PinPort.seek :clear @03:47:19.09 Air.seek :clear @03:47:19.25 Lysis1.seek :clear @03:47:19.99 Lysis2.seek :clear @03:47:20.82 Archive.seek :clear @03:47:21.63 Carousel.in :default @03:47:21.82 Carousel.home.seek 14 @03:47:22.12 Selecting Cartridge 13 @03:47:22.48 Carousel.seek 13 @03:47:23.07 Carousel.reconfigure :align @03:47:23.23 Lysis1.in :fast @03:47:23.33 Lysis1.seek :locked @03:47:25.98 Plunge.seek :locked @03:47:26.19 Air.in :fast @03:47:26.27 Air.seek :locked @03:47:27.36 Carousel.reconfigure :locked @03:47:27.56 Powering cartridge bus @03:47:30.24 Gate.setIR 1750,5000,0,6 @03:47:30.37 Gate.setIR 0,5000,0,6 @03:47:30.46 PuckHeater.configure :default @03:47:30.56 Gate.power :heat,:ON,limit to 5000mA @03:47:30.75 Mounted lysis Cartridge board#J59949 @03:47:30.81 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:47:32.38 SPR.command "read_sequences" @03:47:32.74 SPR.command "stats_on" @03:47:32.92 SPR.command "start 0 1" @03:47:33.63 SPR.start /var/log/esp/spr26aug0203h.spr.bz2 @03:47:35.59 Waiting for baseline SPR stabilization @03:47:36.17 Handoff.select :waste @03:47:36.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:47:43.45 Syringe tare is 51% of full scale at 24C (drifted -0%) @03:47:43.50 PinPort.in :locking @03:47:43.57 PinPort.seek :locked @03:47:44.47 Archive.in :fast @03:47:44.62 Lysis2.in :fast @03:47:44.69 Lysis1.seek 950uL @03:47:44.73 Archive.seek :empty @03:47:44.97 Lysis2.seek 950uL @03:47:53.23 delayUntil "03:57:35.18" @03:57:35.19 Lysis2.in :DAmix @03:57:35.34 Lysis1.in :DAmix @03:57:36.04 Lysis2.seek 700uL @03:57:36.09 Lysis1.seek 700uL @03:58:11.12 delay 5 seconds @03:58:16.14 Handoff.select SPR @03:58:16.67 SPR.command "start 2 14" @03:58:17.82 SPR stabilized @03:58:18.04 delay 20 seconds @03:58:38.07 SPR.command "start 2 0" @03:58:40.65 Waiting up to 11:00 for SPR to enter state 1 @04:00:38.32 SPR.command "hold_on" @04:00:39.70 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @04:00:40.44 Lysis2.seek 400uL @04:00:40.51 Lysis1.seek 400uL @04:01:22.48 delay 15 seconds @04:01:37.51 SPR.command "hold_off" @04:01:40.36 Waiting up to 1:00:00 for SPR to enter state 7 @04:17:55.93 SPRsummary:3.36RIU,9.99RIU,-3.38RIU,9.75RIU after 1924s @04:32:12.98 delay 20 seconds @04:32:33.01 SPR.command "start 2 0" @04:32:36.09 Waiting up to 11:00 for SPR to enter state 1 @04:34:33.76 SPR.command "hold_on" @04:34:35.66 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @04:34:36.43 Lysis2.seek 100uL @04:34:36.47 Lysis1.seek 100uL @04:35:18.50 delay 15 seconds @04:35:33.52 SPR.command "hold_off" @04:35:37.26 Waiting up to 1:00:00 for SPR to enter state 7 @05:06:09.23 SPR analysis completed @05:06:09.89 Ended log spr26aug0203h.spr.bz2 @05:06:09.91 SPR.command "stats_off" @05:06:11.02 Handoff.select :close @05:06:11.57 Gate.power :heat,:OFF @05:06:11.59 Gate.power :cartridge,:OFF @05:06:11.73 PinPort.in :unlocking @05:06:11.86 PinPort.seek :clear @05:06:12.71 Carousel.in :default @05:06:12.87 Carousel.home.seek 13 @05:06:13.24 Plunge.seek :locked @05:06:13.46 Archive.in :unlocking @05:06:13.62 Archive.seek :clear @05:06:13.71 Air.in :unlocking @05:06:13.94 Air.seek :clear @05:06:14.10 Lysis1.in :unlocking @05:06:14.27 Lysis1.seek :clear @05:06:14.34 Lysis2.in :unlocking @05:06:14.49 Lysis2.seek :clear @05:06:19.12 Gate.power :motor,:OFF @05:06:19.68 Gate.power :analytic1,:OFF @05:06:19.71 Gate.power :core,:OFF @05:06:19.74 Gate.power :main,:OFF @05:06:19.78 Safely stopped and ready to power off @05:06:20.06 Gate.power :sampler,:OFF,limit to 3500mA @05:06:20.09 Gate.power :skimmer,:OFF,limit to 3500mA @05:06:23.55 Gate.power :main,:ON,limit to 5000mA @05:06:23.58 Gate.power :analytic1,:ON,limit to 4000mA @05:06:23.71 Powering core bus @05:06:26.05 SPR Module 1, FW Rev. B, FPGA Rev. C @05:06:26.51 Plunge.configure :default @05:06:26.63 SamplePump.configure :default @05:06:26.74 Toroid.configure :default @05:06:26.84 Carousel.configure :default @05:06:26.96 Abs.configure :default @05:06:27.08 PhaseSeparator.configure :default @05:06:27.19 Archive.configure :default @05:06:27.30 Air.configure :default @05:06:27.37 Lysis1.configure :default @05:06:27.45 Lysis2.configure :default @05:06:27.57 PinPort.configure :default @05:06:27.65 All elf controllers running firmware version 4.94 @05:06:27.68 Gate.power :motor,:ON,limit to 5000mA @05:06:28.03 Plunge.home.jog 0 @05:06:28.76 PinPort.home.jog 0 @05:06:28.79 Lysis1.home.jog 0 @05:06:28.88 Archive.home.jog 0 @05:06:28.93 Carousel.home.jog 0 @05:06:28.97 Toroid.home.jog 0 @05:06:29.01 Lysis2.home.jog 0 @05:06:29.06 Air.home.jog 0 @05:06:29.95 Plunge.in :homing @05:06:30.24 Plunge.jog 1490 @05:06:30.47 Lysis2.in :homing @05:06:30.54 Lysis1.in :homing @05:06:30.58 Archive.in :homing @05:06:30.63 PinPort.in :homing @05:06:30.68 Air.in :homing @05:06:30.72 Toroid.in :homing @05:06:30.95 Lysis2.jog 1616 @05:06:31.03 Lysis1.jog 1602 @05:06:31.26 PinPort.jog 1243 @05:06:31.29 Air.jog 1432 @05:06:31.37 Archive.jog 1618 @05:06:31.48 Toroid.jog 1350 @05:06:31.89 Plunge.setRawPosition! 50 @05:06:31.97 Plunge.seek :home @05:06:32.27 Plunge.in :default @05:06:32.56 Lysis1.setRawPosition! 50 @05:06:32.65 Lysis1.seek :home @05:06:32.77 Lysis2.setRawPosition! 50 @05:06:32.85 Lysis2.seek :home @05:06:33.01 Air.setRawPosition! 0 @05:06:33.05 Archive.setRawPosition! 50 @05:06:33.10 Lysis1.in :default @05:06:33.28 Air.seek :home @05:06:33.95 Archive.seek :home @05:06:34.30 Lysis2.in :default @05:06:34.32 PinPort.setRawPosition! 3150 @05:06:34.36 Air.in :default @05:06:34.57 PinPort.seek :home @05:06:34.71 Archive.in :default @05:06:36.21 Toroid.setRawPosition! 0 @05:06:36.30 Toroid.seek :home @05:06:36.40 Toroid.in :default @05:06:38.98 PinPort.in :default @05:06:39.35 Toroid.seek :clear @05:06:39.54 PinPort.in :unlocking @05:06:39.64 PinPort.seek :clear @05:06:43.01 Air.seek :clear @05:06:43.15 Lysis1.seek :clear @05:06:43.90 Lysis2.seek :clear @05:06:44.72 Archive.seek :clear @05:06:45.54 Carousel.in :default @05:06:45.70 Carousel.home.seek 13 @05:06:46.00 Selecting Cartridge 12 @05:06:46.34 Carousel.seek 12 @05:06:46.93 Carousel.reconfigure :align @05:06:47.09 Lysis1.in :fast @05:06:47.17 Lysis1.seek :locked @05:06:49.88 Plunge.seek :locked @05:06:50.07 Air.in :fast @05:06:50.16 Air.seek :locked @05:06:51.25 Carousel.reconfigure :locked @05:06:51.43 Powering cartridge bus @05:06:54.10 Gate.setIR 1750,5000,0,6 @05:06:54.24 Gate.setIR 0,5000,0,6 @05:06:54.33 PuckHeater.configure :default @05:06:54.42 Gate.power :heat,:ON,limit to 5000mA @05:06:54.63 Mounted lysis Cartridge board#J59912 @05:06:54.69 Using default cartridge heat loss curve for HeatingFunctionInCarousel @05:06:56.28 SPR.command "read_sequences" @05:06:56.35 SPR.command "stats_on" @05:06:57.77 SPR.command "start 0 1" @05:06:57.98 SPR.start /var/log/esp/spr26aug0205h.spr.bz2 @05:07:00.00 Waiting for baseline SPR stabilization @05:07:00.02 Handoff.select :waste @05:07:00.57 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @05:07:07.24 Syringe tare is 51% of full scale at 24C (drifted -0%) @05:07:07.29 PinPort.in :locking @05:07:07.37 PinPort.seek :locked @05:07:08.26 Archive.in :fast @05:07:08.37 Lysis2.in :fast @05:07:08.45 Lysis1.seek 950uL @05:07:08.52 Archive.seek :empty @05:07:08.60 Lysis2.seek 950uL @05:07:17.06 delayUntil "05:16:59.57" @05:16:59.59 Lysis2.in :DAmix @05:16:59.74 Lysis1.in :DAmix @05:17:00.47 Lysis2.seek 700uL @05:17:00.51 Lysis1.seek 700uL @05:17:35.54 delay 5 seconds @05:17:40.57 Handoff.select SPR @05:17:41.09 SPR.command "start 2 14" @05:17:42.23 SPR stabilized @05:17:42.41 delay 20 seconds @05:18:02.44 SPR.command "start 2 0" @05:18:05.58 Waiting up to 11:00 for SPR to enter state 1 @05:20:03.67 SPR.command "hold_on" @05:20:05.59 SPR Dilution 1 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:20:06.32 Lysis2.seek 400uL @05:20:06.48 Lysis1.seek 400uL @05:20:48.50 delay 15 seconds @05:21:03.52 SPR.command "hold_off" @05:21:06.65 Waiting up to 1:00:00 for SPR to enter state 7 @05:37:21.28 SPRsummary:1.26RIU,15.1RIU,2.87RIU,16RIU after 1925s @05:51:38.34 delay 20 seconds @05:51:58.36 SPR.command "start 2 0" @05:52:01.45 Waiting up to 11:00 for SPR to enter state 1 @05:53:59.11 SPR.command "hold_on" @05:54:00.49 SPR Dilution 2 of 2 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:54:01.27 Lysis2.seek 100uL @05:54:01.33 Lysis1.seek 100uL @05:54:43.30 delay 15 seconds @05:54:58.32 SPR.command "hold_off" @05:55:01.67 Waiting up to 1:00:00 for SPR to enter state 7 @06:25:34.57 SPR analysis completed @06:25:35.23 Ended log spr26aug0205h.spr.bz2 @06:25:35.26 SPR.command "stats_off" @06:25:36.37 Handoff.select :close @06:25:36.92 Gate.power :heat,:OFF @06:25:36.95 Gate.power :cartridge,:OFF @06:25:37.07 PinPort.in :unlocking @06:25:37.15 PinPort.seek :clear @06:25:38.01 Carousel.in :default @06:25:38.17 Carousel.home.seek 12 @06:25:38.53 Plunge.seek :locked @06:25:38.75 Archive.in :unlocking @06:25:38.90 Archive.seek :clear @06:25:39.03 Air.in :unlocking @06:25:39.19 Air.seek :clear @06:25:39.32 Lysis1.in :unlocking @06:25:40.04 Lysis1.seek :clear @06:25:40.21 Lysis2.in :unlocking @06:25:40.33 Lysis2.seek :clear @06:25:44.98 Gate.power :motor,:OFF @06:25:45.02 Gate.power :analytic1,:OFF @06:25:45.05 Gate.power :core,:OFF @06:25:45.09 Gate.power :main,:OFF @06:25:45.12 Safely stopped and ready to power off @06:25:45.32 Cartridge::None in detached from bill -- Not enough standard cartridges remain