@12:05:47.01PDT17-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:05:48.81 >BREAK< ESP3 Gateway v1.43 for core bus @12:05:48.84 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:05:52.49 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @12:05:53.19 Sea pressure (Abs) tare is 6% of full scale @12:05:56.87 Polling Can every 2:00 @12:05:56.99 Loading /home/esp/esp2/bin/processAll mission @12:05:57.49 Sea pressure (Abs) tare is 6% of full scale @12:05:57.52 Sampling default goal volumes @12:05:57.56 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @12:05:57.94 Gate.power :sampler,:OFF,limit to 3500mA @12:05:57.96 Gate.power :skimmer,:OFF,limit to 3500mA @12:05:58.00 Gate.power :analytic1,:ON,limit to 4000mA @12:05:59.14 Plunge.configure :default @12:05:59.36 SamplePump.configure :default @12:05:59.43 SPR Module 1, FW Rev. B, FPGA Rev. C @12:05:59.54
Toroid.configure :default @12:05:59.68 Carousel.configure :default @12:05:59.73 Carousel.hold @12:05:59.80 Carousel.configure :default @12:05:59.94 Abs.configure :default @12:06:00.07 PhaseSeparator.configure :default @12:06:00.21 Archive.configure :default @12:06:00.34 Air.configure :default @12:06:00.46 Lysis1.configure :default @12:06:00.57 Lysis2.configure :default @12:06:00.73 PinPort.configure :default @12:06:00.78 All elf controllers running firmware version 4.92 @12:06:01.04 Plunge.in :default @12:06:01.42 Plunge.home.jog 0 @12:06:01.63 PinPort.in :default @12:06:01.70 Lysis2.in :default @12:06:01.73 Air.in :default @12:06:01.76 Archive.in :default @12:06:01.78 Toroid.in :default @12:06:01.83 Lysis1.in :default @12:06:02.04 PinPort.home.jog 0 @12:06:02.12 Air.home.jog 0 @12:06:02.14 Lysis2.home.jog 0 @12:06:02.17 Lysis1.home.jog 0 @12:06:02.19 Toroid.home.jog 0 @12:06:02.22 Archive.home.jog 0 @12:06:03.78 PinPort.in :homing @12:06:03.86 Lysis2.in :homing @12:06:03.89 Air.in :homing @12:06:03.91 Toroid.in :homing @12:06:03.93 Archive.in :homing @12:06:03.96 Plunge.in :homing @12:06:03.99 Lysis1.in :homing @12:06:04.20 PinPort.jog -107 @12:06:04.31 Air.jog 1374 @12:06:04.35 Lysis2.jog 1404 @12:06:04.39 Toroid.jog 628 @12:06:04.85 Plunge.jog 162 @12:06:04.91 Archive.jog 1399 @12:06:04.94 Lysis1.jog 1397 @12:06:05.38 PinPort.setRawPosition! 0 @12:06:05.43 PinPort.seek :home @12:06:05.52 PinPort.in :default @12:06:05.63 Plunge.setRawPosition! 50 @12:06:05.69 Plunge.seek :home @12:06:05.97 Plunge.in :default @12:06:06.22 Air.setRawPosition! 50 @12:06:06.31 Lysis2.setRawPosition! 50 @12:06:06.35 Air.seek :home @12:06:06.46 Lysis2.seek :home @12:06:06.67 Archive.setRawPosition! 50 @12:06:06.72 Lysis1.setRawPosition! 50 @12:06:06.81 Lysis1.seek :home @12:06:06.87 Archive.seek :home @12:06:07.08 Air.in :default @12:06:07.10 Lysis2.in :default @12:06:07.28 Archive.in :default @12:06:07.32 Lysis1.in :default @12:06:07.46 Toroid.setRawPosition! 0 @12:06:07.52 Toroid.seek :home @12:06:07.62 Toroid.in :default @12:06:07.85 Toroid.seek :clear @12:06:08.69 Session Begun @12:06:09.72
Carousel.in :default @12:06:09.89 Carousel.home.seek 58 @12:06:10.18 Selecting Cartridge 58 @12:06:10.51 Carousel.seek 58 @12:06:10.82 Lysis1.in :fast @12:06:10.91 Lysis1.seek :locked @12:06:13.57 Plunge.seek :locked @12:06:13.78 Air.in :fast @12:06:13.86 Air.seek :locked @12:06:14.86
Carousel.reconfigure :locked @12:06:15.03 Powering cartridge bus @12:06:17.70 Gate.setIR 1750,5000,0,6 @12:06:17.82 Gate.setIR 0,5000,0,6 @12:06:17.91 PuckHeater.configure :default @12:06:18.00 Gate.power :heat,:ON,limit to 5000mA @12:06:18.73 Mounted lysis Cartridge @12:06:18.76 WARNING: Using default puck temperature sensor calibration @12:06:18.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:06:20.40 SPR.command "read_sequences" @12:06:21.09 SPR.command "stats_on" @12:06:21.75 SPR.command "start 0 1" @12:06:21.97 SPR.start /var/log/esp/spr24sep1712h.spr.bz2 @12:06:24.45 Waiting for baseline SPR stabilization @12:06:24.46 Handoff.select :waste @12:06:24.65 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:06:31.29 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:06:31.32 PinPort.in :locking @12:06:31.43 PinPort.seek :locked @12:06:31.95 Archive.in :fast @12:06:32.09 Lysis2.in :fast @12:06:32.15 Lysis1.seek 1100uL @12:06:32.24 Archive.seek :full @12:06:32.43 Lysis2.seek 1100uL @12:06:36.75 delayUntil "12:16:24.41" @12:16:24.43 Lysis2.in :DAmix @12:16:24.57 Lysis1.in :DAmix @12:16:25.16 Lysis2.seek 950uL @12:16:25.26 Lysis1.seek 950uL @12:16:46.07 delay 5 seconds @12:16:51.09 Handoff.select SPR @12:16:51.25 SPR.command "start 2 14" @12:16:51.97 SPR stabilized @12:16:52.16 delay 10 seconds @12:17:02.18 SPR.command "start 2 0" @12:17:05.34 Waiting up to 11:00 for SPR to enter state 1 @12:19:03.00 SPR.command "hold_on" @12:19:04.40 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @12:19:05.09 Lysis2.seek 500uL @12:19:05.13 Lysis1.seek 500uL @12:20:07.33 delay 15 seconds @12:20:22.37 SPR.command "hold_off" @12:20:24.91 Waiting up to 1:00:00 for SPR to enter state 7 @12:36:40.47 SPRsummary:12.9RIU,154RIU,376RIU,38.6RIU after 1920s @12:50:57.51 delay 10 seconds @12:51:07.53 SPR.command "start 2 0" @12:51:10.24 Waiting up to 11:00 for SPR to enter state 1 @12:53:07.90 SPR.command "hold_on" @12:53:09.28 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:53:10.33 Lysis2.seek 275uL @12:53:10.43 Lysis1.seek 275uL @12:53:41.57 delay 15 seconds @12:53:56.59 SPR.command "hold_off" @12:53:59.54 Waiting up to 1:00:00 for SPR to enter state 7 @13:24:32.15 delay 10 seconds @13:24:42.17 SPR.command "start 2 0" @13:24:44.85 Waiting up to 11:00 for SPR to enter state 1 @13:26:42.53 SPR.command "hold_on" @13:26:43.90 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:26:44.57 Lysis2.seek 50uL @13:26:44.66 Lysis1.seek 50uL @13:27:15.85 delay 15 seconds @13:27:30.87 SPR.command "hold_off" @13:27:34.15 Waiting up to 1:00:00 for SPR to enter state 7 @13:58:06.71 Additional SPR Analyses Skipped due to Insufficient Volume @13:58:06.95 Intake.close @13:58:07.00 Exhaust.close @13:58:07.14 Bypass.close @13:58:07.23 Handoff.select :close @13:58:07.50
Gate.power :heat,:OFF @13:58:07.53 Gate.power :cartridge,:OFF @13:58:07.69 PinPort.in :unlocking @13:58:07.77 PinPort.seek :clear @13:58:08.27 Carousel.in :default @13:58:08.42 Carousel.home.seek 58 @13:58:08.81 Plunge.seek :locked @13:58:09.01 Archive.in :unlocking @13:58:09.17 Archive.seek :clear @13:58:09.28 Air.in :unlocking @13:58:09.48 Air.seek :clear @13:58:09.64 Lysis1.in :unlocking @13:58:09.79 Lysis1.seek :clear @13:58:09.95 Lysis2.in :unlocking @13:58:10.04 Lysis2.seek :clear @13:58:30.33 Ended log spr24sep1712h.spr.bz2 @13:58:30.35
SPR.command "stats_off" @13:58:31.29 Gate.power :analytic1,:OFF @13:58:31.31 Gate.power :motor,:OFF @13:58:31.35 Gate.power :core,:OFF @13:58:31.38 Gate.power :main,:OFF @13:58:31.42 Safely stopped and ready to power off @13:58:32.12 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:58:32.22 Session Ended @12:23:04.67PDT18-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:23:06.38 >BREAK< ESP3 Gateway v1.43 for core bus @12:23:06.41 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:23:09.88 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @12:23:10.55 Sea pressure (Abs) tare is 6% of full scale @12:23:14.16 Polling Can every 2:00 @12:23:14.21 Loading /home/esp/esp2/bin/processAll mission @12:23:14.45 Sea pressure (Abs) tare is 6% of full scale @12:23:14.46 Sampling default goal volumes @12:23:14.48 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @12:23:14.65 Gate.power :sampler,:OFF,limit to 3500mA @12:23:14.66 Gate.power :skimmer,:OFF,limit to 3500mA @12:23:14.67 Gate.power :analytic1,:ON,limit to 4000mA @12:23:15.69 SPR Module 1, FW Rev. B, FPGA Rev. C @12:23:15.80
Plunge.configure :default @12:23:16.01 SamplePump.configure :default @12:23:16.15 Toroid.configure :default @12:23:16.28 Carousel.configure :default @12:23:16.41 Abs.configure :default @12:23:16.55 PhaseSeparator.configure :default @12:23:16.68 Archive.configure :default @12:23:16.81 Air.configure :default @12:23:16.92 Lysis1.configure :default @12:23:17.03 Lysis2.configure :default @12:23:17.16 PinPort.configure :default @12:23:17.21 All elf controllers running firmware version 4.92 @12:23:17.53 Plunge.in :default @12:23:18.02 Plunge.home.jog 0 @12:23:18.26 Lysis2.in :default @12:23:18.31 Lysis1.in :default @12:23:18.35 Archive.in :default @12:23:18.37 Carousel.in :default @12:23:18.39 PinPort.in :default @12:23:18.41 Air.in :default @12:23:18.43 Toroid.in :default @12:23:18.69 Lysis1.home.jog 0 @12:23:18.73 Lysis2.home.jog 0 @12:23:18.75 Archive.home.jog 0 @12:23:18.77 Carousel.home.jog 0 @12:23:18.79 PinPort.home.jog 0 @12:23:18.81 Air.home.jog 0 @12:23:18.83 Toroid.home.jog 0 @12:23:19.89 Lysis1.in :homing @12:23:19.98 Air.in :homing @12:23:20.02 Toroid.in :homing @12:23:20.04 Lysis2.in :homing @12:23:20.06 Plunge.in :homing @12:23:20.08 Archive.in :homing @12:23:20.16 PinPort.in :homing @12:23:20.33 Lysis1.jog 1392 @12:23:20.41 Air.jog 1376 @12:23:20.45 Toroid.jog 632 @12:23:20.87 Lysis2.jog 1406 @12:23:20.90 Archive.jog 1397 @12:23:20.93 Plunge.jog 163 @12:23:20.99 PinPort.jog -110 @12:23:21.55 PinPort.setRawPosition! 0 @12:23:21.60 PinPort.seek :home @12:23:21.71 PinPort.in :default @12:23:21.79 Plunge.setRawPosition! 50 @12:23:21.85 Plunge.seek :home @12:23:22.13 Lysis1.setRawPosition! 50 @12:23:22.16 Plunge.in :default @12:23:22.28 Lysis1.seek :home @12:23:22.38 Lysis2.setRawPosition! 50 @12:23:22.44 Air.setRawPosition! 50 @12:23:22.49 Lysis2.seek :home @12:23:22.61 Air.seek :home @12:23:22.73 Archive.setRawPosition! 50 @12:23:22.75 Lysis1.in :default @12:23:22.89 Archive.seek :home @12:23:23.01 Air.in :default @12:23:23.03 Lysis2.in :default @12:23:23.17 Archive.in :default @12:23:23.26 Toroid.setRawPosition! 0 @12:23:23.31 Toroid.seek :home @12:23:23.40 Toroid.in :default @12:23:23.55 Toroid.seek :clear @12:23:25.27
Carousel.in :default @12:23:25.41 Carousel.home.seek 58 @12:23:25.84 Selecting Cartridge 58 @12:23:26.12 Carousel.seek 58 @12:23:26.37 Lysis1.in :fast @12:23:26.42 Lysis1.seek :locked @12:23:29.03 Plunge.seek :locked @12:23:29.19 Air.in :fast @12:23:29.24 Air.seek :locked @12:23:30.21
Carousel.reconfigure :locked @12:23:30.36 Powering cartridge bus @12:23:33.03 Gate.setIR 1750,5000,0,6 @12:23:33.15 Gate.setIR 0,5000,0,6 @12:23:33.21 PuckHeater.configure :default @12:23:33.27 Gate.power :heat,:ON,limit to 5000mA @12:23:33.94 Mounted lysis Cartridge @12:23:33.97 WARNING: Using default puck temperature sensor calibration @12:23:33.98 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:23:35.51 SPR.command "read_sequences" @12:23:35.57 SPR.command "stats_on" @12:23:36.90 SPR.command "start 0 1" @12:23:37.15 SPR.start /var/log/esp/spr24sep1812h.spr.bz2 @12:23:39.19 Waiting for baseline SPR stabilization @12:23:39.21 Handoff.select :waste @12:23:39.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:23:45.98 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:23:46.00 PinPort.in :locking @12:23:46.05 PinPort.seek :locked @12:23:46.55 Archive.in :fast @12:23:46.64 Lysis2.in :fast @12:23:46.69 Lysis1.seek 1100uL @12:23:46.72 Archive.seek :full @12:23:46.84 Lysis2.seek 1100uL @12:23:51.16 delayUntil "12:33:38.77" @12:24:17.86 Session Begun @12:33:38.79 Lysis2.in :DAmix @12:33:38.93 Lysis1.in :DAmix @12:33:39.52 Lysis2.seek 950uL @12:33:39.63 Lysis1.seek 950uL @12:34:00.49 delay 5 seconds @12:34:05.51 Handoff.select SPR @12:34:05.67 SPR.command "start 2 14" @12:34:06.28 SPR stabilized @12:34:06.47 delay 10 seconds @12:34:16.49 SPR.command "start 2 0" @12:34:19.65 Waiting up to 11:00 for SPR to enter state 1 @12:36:17.30 SPR.command "hold_on" @12:36:18.71 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @12:36:19.36 Lysis2.seek 500uL @12:36:19.46 Lysis1.seek 500uL @12:37:21.68 delay 15 seconds @12:37:36.71 SPR.command "hold_off" @12:37:40.17 Waiting up to 1:00:00 for SPR to enter state 7 @12:49:56.42 Session Begun @12:50:04.41 -> showlog @12:53:55.73 SPRsummary:9.88RIU,127RIU,295RIU,21.3RIU after 1920s @13:08:12.76 delay 10 seconds @13:08:22.78 SPR.command "start 2 0" @13:08:25.50 Waiting up to 11:00 for SPR to enter state 1 @13:10:23.15 SPR.command "hold_on" @13:10:24.55 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:10:25.24 Lysis2.seek 275uL @13:10:25.30 Lysis1.seek 275uL @13:10:56.60 delay 15 seconds @13:11:11.62 SPR.command "hold_off" @13:11:14.79 Waiting up to 1:00:00 for SPR to enter state 7 @13:41:47.39 delay 10 seconds @13:41:57.43 SPR.command "start 2 0" @13:42:00.63 Waiting up to 11:00 for SPR to enter state 1 @13:43:58.72 SPR.command "hold_on" @13:44:00.11 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:44:00.79 Lysis2.seek 50uL @13:44:00.86 Lysis1.seek 50uL @13:44:32.09 delay 15 seconds @13:44:47.12 SPR.command "hold_off" @13:44:50.35 Waiting up to 1:00:00 for SPR to enter state 7 @14:15:22.89 Additional SPR Analyses Skipped due to Insufficient Volume @14:15:23.15 Intake.close @14:15:23.26 Exhaust.close @14:15:23.35 Bypass.close @14:15:23.51 Handoff.select :close @14:15:23.86
Gate.power :heat,:OFF @14:15:23.88 Gate.power :cartridge,:OFF @14:15:24.03 PinPort.in :unlocking @14:15:24.11 PinPort.seek :clear @14:15:24.66 Carousel.in :default @14:15:24.87 Carousel.home.seek 58 @14:15:25.25 Plunge.seek :locked @14:15:25.52 Archive.in :unlocking @14:15:25.70 Archive.seek :clear @14:15:25.84 Air.in :unlocking @14:15:26.00 Air.seek :clear @14:15:26.15 Lysis1.in :unlocking @14:15:26.33 Lysis1.seek :clear @14:15:26.41 Lysis2.in :unlocking @14:15:26.62 Lysis2.seek :clear @14:15:46.99 Ended log spr24sep1812h.spr.bz2 @14:15:47.02
SPR.command "stats_off" @14:15:48.03 Gate.power :analytic1,:OFF @14:15:48.06 Gate.power :motor,:OFF @14:15:48.11 Gate.power :core,:OFF @14:15:48.17 Gate.power :main,:OFF @14:15:48.20 Safely stopped and ready to power off @14:15:48.98 Cartridge::None in MAIN -- Not enough standard cartridges remain @14:15:49.09 Session Ended @14:15:49.15 Session Ended @15:41:04.03PDT18-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @15:41:05.76 >BREAK< ESP3 Gateway v1.43 for core bus @15:41:05.79 >BREAK< ESP3 Gateway v1.43 for cartridge bus @15:41:09.47 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @15:41:10.15 Sea pressure (Abs) tare is 6% of full scale @15:41:13.89 Polling Can every 2:00 @15:41:13.94 Loading /home/esp/esp2/bin/processAll mission @15:41:14.18 Sea pressure (Abs) tare is 6% of full scale @15:41:14.20 Sampling default goal volumes @15:41:14.22 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @15:41:14.38 Gate.power :sampler,:OFF,limit to 3500mA @15:41:14.39 Gate.power :skimmer,:OFF,limit to 3500mA @15:41:14.44 Gate.power :analytic1,:ON,limit to 4000mA @15:41:15.03 SPR Module 1, FW Rev. B, FPGA Rev. C @15:41:15.21
Plunge.configure :default @15:41:15.34 SamplePump.configure :default @15:41:15.52 Toroid.configure :default @15:41:15.57 Toroid.hold @15:41:15.64 Toroid.configure :default @15:41:15.77 Carousel.configure :default @15:41:15.82 Carousel.hold @15:41:15.89 Carousel.configure :default @15:41:16.03 Abs.configure :default @15:41:16.16 PhaseSeparator.configure :default @15:41:16.30 Archive.configure :default @15:41:16.43 Air.configure :default @15:41:16.55 Lysis1.configure :default @15:41:16.66 Lysis2.configure :default @15:41:16.80 PinPort.configure :default @15:41:16.85 All elf controllers running firmware version 4.92 @15:41:17.66 Toroid.seek :clear @15:41:17.83
Carousel.in :default @15:41:17.94 Carousel.home.seek 58 @15:41:18.21 Selecting Cartridge 58 @15:41:18.51 Carousel.seek 58 @15:41:18.75 Lysis1.in :fast @15:41:18.81 Lysis1.seek :locked @15:41:21.43 Plunge.seek :locked @15:41:22.07 Air.in :fast @15:41:22.13 Air.seek :locked @15:41:23.10
Carousel.reconfigure :locked @15:41:23.25 Powering cartridge bus @15:41:25.92 Gate.setIR 1750,5000,0,6 @15:41:26.04 Gate.setIR 0,5000,0,6 @15:41:26.11 PuckHeater.configure :default @15:41:26.17 Gate.power :heat,:ON,limit to 5000mA @15:41:26.85 Mounted lysis Cartridge @15:41:26.88 WARNING: Using default puck temperature sensor calibration @15:41:26.89 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:41:28.44 SPR.command "read_sequences" @15:41:29.13 SPR.command "stats_on" @15:41:29.29 SPR.command "start 0 1" @15:41:30.00 SPR.start /var/log/esp/spr24sep1815h.spr.bz2 @15:41:31.99 Waiting for baseline SPR stabilization @15:41:32.01 Handoff.select :waste @15:41:32.14 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:41:38.77 Syringe tare is 51% of full scale at 22C (drifted 0%) @15:41:38.79 PinPort.in :locking @15:41:38.84 PinPort.seek :locked @15:41:39.34 Archive.in :fast @15:41:39.48 Lysis2.in :fast @15:41:39.53 Archive.seek :full @15:41:39.58 Lysis1.seek 1100uL @15:41:39.72 Lysis2.seek 1100uL @15:41:44.01 delayUntil "15:51:31.52" @15:51:31.54 Lysis2.in :DAmix @15:51:31.65 Lysis1.in :DAmix @15:51:32.21 Lysis2.seek 950uL @15:51:32.30 Lysis1.seek 950uL @15:51:53.10 delay 5 seconds @15:51:58.12 Handoff.select SPR @15:51:58.25 SPR.command "start 2 14" @15:51:59.07 SPR stabilized @15:51:59.21 delay 10 seconds @15:52:09.24 SPR.command "start 2 0" @15:52:12.44 Waiting up to 11:00 for SPR to enter state 1 @15:54:10.09 SPR.command "hold_on" @15:54:11.50 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:54:12.09 Lysis2.seek 500uL @15:54:12.15 Lysis1.seek 500uL @15:55:14.31 delay 15 seconds @15:55:29.34 SPR.command "hold_off" @15:55:32.50 Waiting up to 1:00:00 for SPR to enter state 7 @15:56:16.31 Session Begun @15:56:21.53 -> showlog @16:11:48.51 SPRsummary:-2.8RIU,128RIU,270RIU,-1.01RIU after 1920s @16:26:05.58 delay 10 seconds @16:26:15.61 SPR.command "start 2 0" @16:26:18.28 Waiting up to 11:00 for SPR to enter state 1 @16:28:15.93 SPR.command "hold_on" @16:28:17.33 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:28:18.06 Lysis2.seek 275uL @16:28:18.12 Lysis1.seek 275uL @16:28:49.33 delay 15 seconds @16:29:04.35 SPR.command "hold_off" @16:29:07.58 Waiting up to 1:00:00 for SPR to enter state 7 @16:59:40.20 delay 10 seconds @16:59:50.22 SPR.command "start 2 0" @16:59:52.90 Waiting up to 11:00 for SPR to enter state 1 @17:01:50.56 SPR.command "hold_on" @17:01:51.96 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @17:01:52.60 Lysis2.seek 50uL @17:01:52.68 Lysis1.seek 50uL @17:02:23.84 delay 15 seconds @17:02:38.86 SPR.command "hold_off" @17:02:42.72 Waiting up to 1:00:00 for SPR to enter state 7 @17:33:15.69 Additional SPR Analyses Skipped due to Insufficient Volume @17:33:15.94 Intake.close @17:33:15.99 Exhaust.close @17:33:16.06 Bypass.close @17:33:16.19 Handoff.select :close @17:33:16.50
Gate.power :heat,:OFF @17:33:16.55 Gate.power :cartridge,:OFF @17:33:16.69 PinPort.in :unlocking @17:33:16.77 PinPort.seek :clear @17:33:17.27 Carousel.in :default @17:33:17.47 Carousel.home.seek 58 @17:33:17.86 Plunge.seek :locked @17:33:18.06 Archive.in :unlocking @17:33:18.22 Archive.seek :clear @17:33:18.29 Air.in :unlocking @17:33:18.52 Air.seek :clear @17:33:18.68 Lysis1.in :unlocking @17:33:18.83 Lysis1.seek :clear @17:33:18.97 Lysis2.in :unlocking @17:33:19.06 Lysis2.seek :clear @17:33:39.59 Ended log spr24sep1815h.spr.bz2 @17:33:39.61
SPR.command "stats_off" @17:33:39.88 Gate.power :analytic1,:OFF @17:33:39.90 Gate.power :motor,:OFF @17:33:39.94 Gate.power :core,:OFF @17:33:39.98 Gate.power :main,:OFF @17:33:40.02 Safely stopped and ready to power off @17:33:40.71 Cartridge::None in MAIN -- Not enough standard cartridges remain @17:33:40.80 Session Ended nohup: procelAll: No such file or directory @10:06:46.78PDT19-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @10:06:48.54 >BREAK< ESP3 Gateway v1.43 for core bus @10:06:48.56 >BREAK< ESP3 Gateway v1.43 for cartridge bus @10:06:52.13 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @10:06:52.80 Sea pressure (Abs) tare is 6% of full scale @10:06:56.42 Polling Can every 2:00 @10:06:56.47 Loading /home/esp/esp2/bin/processAll mission @10:06:56.71 Sea pressure (Abs) tare is 6% of full scale @10:06:56.72 Sampling default goal volumes @10:06:56.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @10:06:56.91 Gate.power :sampler,:OFF,limit to 3500mA @10:06:56.92 Gate.power :skimmer,:OFF,limit to 3500mA @10:06:56.97 Gate.power :analytic1,:ON,limit to 4000mA @10:06:57.69 Plunge.configure :default @10:06:57.83 SamplePump.configure :default @10:06:57.93 SPR Module 1, FW Rev. B, FPGA Rev. C @10:06:58.03
Toroid.configure :default @10:06:58.17 Carousel.configure :default @10:06:58.31 Abs.configure :default @10:06:58.45 PhaseSeparator.configure :default @10:06:58.59 Archive.configure :default @10:06:58.84 Air.configure :default @10:06:58.96 Lysis1.configure :default @10:06:59.08 Lysis2.configure :default @10:06:59.22 PinPort.configure :default @10:06:59.27 All elf controllers running firmware version 4.92 @10:06:59.54 Plunge.in :default @10:07:00.05 Plunge.home.jog 0 @10:07:00.27 Lysis2.in :default @10:07:00.32 Lysis1.in :default @10:07:00.36 Air.in :default @10:07:00.38 Archive.in :default @10:07:00.40 Carousel.in :default @10:07:00.43 Toroid.in :default @10:07:00.44 PinPort.in :default @10:07:00.73 Lysis1.home.jog 0 @10:07:00.82 Lysis2.home.jog 0 @10:07:00.85 Air.home.jog 0 @10:07:00.87 Archive.home.jog 0 @10:07:00.89 PinPort.home.jog 0 @10:07:00.91 Carousel.home.jog 0 @10:07:00.95 Toroid.home.jog 0 @10:07:02.00 Lysis2.in :homing @10:07:02.11 Lysis1.in :homing @10:07:02.14 Toroid.in :homing @10:07:02.15 Archive.in :homing @10:07:02.19 PinPort.in :homing @10:07:02.22 Air.in :homing @10:07:02.26 Plunge.in :homing @10:07:02.47 Lysis2.jog 1409 @10:07:02.55 Lysis1.jog 1396 @10:07:02.99 Archive.jog 1401 @10:07:03.04 PinPort.jog -109 @10:07:03.07 Toroid.jog 632 @10:07:03.11 Air.jog 1375 @10:07:03.30 Plunge.jog 159 @10:07:03.69 PinPort.setRawPosition! 0 @10:07:03.74 PinPort.seek :home @10:07:03.84 PinPort.in :default @10:07:04.00 Plunge.setRawPosition! 50 @10:07:04.06 Plunge.seek :home @10:07:04.31 Lysis2.setRawPosition! 50 @10:07:04.41 Lysis1.setRawPosition! 50 @10:07:04.45 Archive.setRawPosition! 50 @10:07:04.47 Lysis2.seek :home @10:07:04.49 Plunge.in :default @10:07:04.68 Lysis1.seek :home @10:07:04.70 Archive.seek :home @10:07:04.97 Air.setRawPosition! 50 @10:07:04.99 Lysis2.in :default @10:07:05.11 Air.seek :home @10:07:05.22 Lysis1.in :default @10:07:05.24 Archive.in :default @10:07:05.39 Air.in :default @10:07:05.53 Toroid.setRawPosition! 0 @10:07:05.60 Toroid.seek :home @10:07:05.69 Toroid.in :default @10:07:05.78 Toroid.seek :clear @10:07:07.49
Carousel.in :default @10:07:07.62 Carousel.home.seek 58 @10:07:07.89 Selecting Cartridge 58 @10:07:08.19 Carousel.seek 58 @10:07:08.45 Lysis1.in :fast @10:07:08.51 Lysis1.seek :locked @10:07:11.25 Plunge.seek :locked @10:07:11.59 Air.in :fast @10:07:11.69 Air.seek :locked @10:07:11.95 Session Begun @10:07:12.71
Carousel.reconfigure :locked @10:07:12.89 Powering cartridge bus @10:07:15.56 Gate.setIR 1750,5000,0,6 @10:07:15.69 Gate.setIR 0,5000,0,6 @10:07:15.78 PuckHeater.configure :default @10:07:15.87 Gate.power :heat,:ON,limit to 5000mA @10:07:16.54 Mounted lysis Cartridge @10:07:16.59 WARNING: Using default puck temperature sensor calibration @10:07:16.61 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:07:18.20 SPR.command "read_sequences" @10:07:18.64 SPR.command "stats_on" @10:07:19.38 SPR.command "start 0 1" @10:07:20.09 SPR.start /var/log/esp/spr24sep1910h.spr.bz2 @10:07:21.99 Waiting for baseline SPR stabilization @10:07:22.01 Handoff.select :waste @10:07:22.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:07:28.84 Syringe tare is 51% of full scale at 22C (drifted -0%) @10:07:28.90 PinPort.in :locking @10:07:29.00 PinPort.seek :locked @10:07:29.53 Archive.in :fast @10:07:29.70 Lysis2.in :fast @10:07:29.83 Lysis1.seek 1100uL @10:07:29.87 Archive.seek :full @10:07:30.07 Lysis2.seek 1100uL @10:07:34.35 delayUntil "10:17:21.95" @10:17:21.97 Lysis2.in :DAmix @10:17:22.11 Lysis1.in :DAmix @10:17:22.67 Lysis2.seek 950uL @10:17:22.72 Lysis1.seek 950uL @10:17:43.63 delay 5 seconds @10:17:48.65 Handoff.select SPR @10:17:48.81 SPR.command "start 2 14" @10:17:49.53 SPR stabilized @10:17:49.71 delay 10 seconds @10:17:59.73 SPR.command "start 2 0" @10:18:02.90 Waiting up to 11:00 for SPR to enter state 1 @10:20:00.54 SPR.command "hold_on" @10:20:01.95 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @10:20:02.60 Lysis2.seek 500uL @10:20:02.65 Lysis1.seek 500uL @10:21:04.84 delay 15 seconds @10:21:19.86 SPR.command "hold_off" @10:21:22.46 Waiting up to 1:00:00 for SPR to enter state 7 @10:37:38.01 SPRsummary:6.18RIU,138RIU,288RIU,20.9RIU after 1920s @10:51:55.06 delay 10 seconds @10:52:05.09 SPR.command "start 2 0" @10:52:07.79 Waiting up to 11:00 for SPR to enter state 1 @10:54:05.46 SPR.command "hold_on" @10:54:06.84 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @10:54:07.56 Lysis2.seek 275uL @10:54:07.60 Lysis1.seek 275uL @10:54:38.80 delay 15 seconds @10:54:53.82 SPR.command "hold_off" @10:54:57.09 Waiting up to 1:00:00 for SPR to enter state 7 @11:25:29.69 delay 10 seconds @11:25:39.71 SPR.command "start 2 0" @11:25:42.41 Waiting up to 11:00 for SPR to enter state 1 @11:27:40.07 SPR.command "hold_on" @11:27:41.47 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @11:27:42.12 Lysis2.seek 50uL @11:27:42.21 Lysis1.seek 50uL @11:28:13.37 delay 15 seconds @11:28:28.39 SPR.command "hold_off" @11:28:31.71 Waiting up to 1:00:00 for SPR to enter state 7 @11:59:04.25 Additional SPR Analyses Skipped due to Insufficient Volume @11:59:04.47 Intake.close @11:59:04.57 Exhaust.close @11:59:04.67 Bypass.close @11:59:04.73 Handoff.select :close @11:59:05.03
Gate.power :heat,:OFF @11:59:05.07 Gate.power :cartridge,:OFF @11:59:05.22 PinPort.in :unlocking @11:59:05.29 PinPort.seek :clear @11:59:05.81 Carousel.in :default @11:59:05.96 Carousel.home.seek 58 @11:59:06.35 Plunge.seek :locked @11:59:06.57 Archive.in :unlocking @11:59:06.74 Archive.seek :clear @11:59:06.90 Air.in :unlocking @11:59:07.00 Air.seek :clear @11:59:07.15 Lysis1.in :unlocking @11:59:07.31 Lysis1.seek :clear @11:59:07.50 Lysis2.in :unlocking @11:59:07.59 Lysis2.seek :clear @11:59:28.48 Ended log spr24sep1910h.spr.bz2 @11:59:28.51
SPR.command "stats_off" @11:59:29.34 Gate.power :analytic1,:OFF @11:59:29.36 Gate.power :motor,:OFF @11:59:29.40 Gate.power :core,:OFF @11:59:29.47 Gate.power :main,:OFF @11:59:29.51 Safely stopped and ready to power off @11:59:30.22 Cartridge::None in MAIN -- Not enough standard cartridges remain @11:59:30.31 Session Ended @13:17:34.44PDT19-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:17:36.15 >BREAK< ESP3 Gateway v1.43 for core bus @13:17:36.18 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:17:39.78 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @13:17:40.49 Sea pressure (Abs) tare is 6% of full scale @13:17:44.26 Polling Can every 2:00 @13:17:44.30 Loading /home/esp/esp2/bin/processAll mission @13:17:44.53 Sea pressure (Abs) tare is 6% of full scale @13:17:44.54 Sampling default goal volumes @13:17:44.56 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:17:44.61 Gate.power :sampler,:OFF,limit to 3500mA @13:17:44.62 Gate.power :skimmer,:OFF,limit to 3500mA @13:17:44.64 Gate.power :main,:ON,limit to 5000mA @13:17:44.65 Gate.power :analytic1,:ON,limit to 4000mA @13:17:44.84 Powering core bus @13:17:47.08 SPR Module 1, FW Rev. B, FPGA Rev. C @13:17:47.62
Plunge.configure :default @13:17:47.69 SamplePump.configure :default @13:17:47.77 Toroid.configure :default @13:17:47.85 Carousel.configure :default @13:17:47.93 Abs.configure :default @13:17:48.00 PhaseSeparator.configure :default @13:17:48.08 Archive.configure :default @13:17:48.15 Air.configure :default @13:17:48.21 Lysis1.configure :default @13:17:48.26 Lysis2.configure :default @13:17:48.34 PinPort.configure :default @13:17:48.39 All elf controllers running firmware version 4.92 @13:17:48.41 Gate.power :motor,:ON,limit to 5000mA @13:17:48.58 Plunge.home.jog 0 @13:17:49.32 PinPort.home.jog 0 @13:17:49.42 Lysis2.home.jog 0 @13:17:49.45 Air.home.jog 0 @13:17:49.53 Carousel.home.jog 0 @13:17:49.60 Lysis1.home.jog 0 @13:17:49.73 Toroid.home.jog 0 @13:17:49.77 Archive.home.jog 0 @13:17:50.95 Plunge.in :homing @13:17:51.27 Plunge.jog 1607 @13:17:51.34 Lysis2.in :homing @13:17:51.38 Air.in :homing @13:17:51.40 Toroid.in :homing @13:17:51.46 PinPort.in :homing @13:17:51.48 Lysis1.in :homing @13:17:51.50 Archive.in :homing @13:17:51.76 Lysis2.jog 1406 @13:17:51.86 Air.jog 1381 @13:17:51.93 PinPort.jog -110 @13:17:51.99 Archive.jog 1399 @13:17:52.03 Toroid.jog 627 @13:17:52.07 Lysis1.jog 1400 @13:17:52.41 PinPort.setRawPosition! 0 @13:17:52.46 PinPort.seek :home @13:17:52.55 PinPort.in :default @13:17:52.83 Plunge.setRawPosition! 50 @13:17:52.94 Plunge.seek :home @13:17:53.80 Lysis1.setRawPosition! 50 @13:17:53.93 Air.setRawPosition! 50 @13:17:53.95 Archive.setRawPosition! 50 @13:17:53.97 Lysis2.setRawPosition! 50 @13:17:54.16 Lysis1.seek :home @13:17:54.21 Air.seek :home @13:17:54.25 Archive.seek :home @13:17:54.34 Lysis2.seek :home @13:17:54.68 Lysis1.in :default @13:17:54.73 Toroid.setRawPosition! 0 @13:17:54.77 Plunge.in :default @13:17:54.89 Toroid.seek :home @13:17:55.08 Air.in :default @13:17:55.13 Archive.in :default @13:17:55.15 Lysis2.in :default @13:17:55.17 Toroid.in :default @13:17:55.64 Toroid.seek :clear @13:17:57.37
Carousel.in :default @13:17:57.52 Carousel.home.seek 58 @13:17:57.80 Selecting Cartridge 57 @13:17:58.10 Carousel.seek 57 @13:17:58.75 Lysis1.in :fast @13:17:58.86 Lysis1.seek :locked @13:17:59.49 Session Begun @13:18:01.58 Plunge.seek :locked @13:18:01.80 Air.in :fast @13:18:01.87 Air.seek :locked @13:18:02.87
Carousel.reconfigure :locked @13:18:03.06 Powering cartridge bus @13:18:05.72 Gate.setIR 1750,5000,0,6 @13:18:05.86 Gate.setIR 0,5000,0,6 @13:18:05.95 PuckHeater.configure :default @13:18:06.04 Gate.power :heat,:ON,limit to 5000mA @13:18:06.73 Mounted lysis Cartridge @13:18:06.76 WARNING: Using default puck temperature sensor calibration @13:18:06.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:18:08.37 SPR.command "read_sequences" @13:18:08.73 SPR.command "stats_on" @13:18:08.90 SPR.command "start 0 1" @13:18:09.61 SPR.start /var/log/esp/spr24sep1913h.spr.bz2 @13:18:11.59 Waiting for baseline SPR stabilization @13:18:11.61 Handoff.select :waste @13:18:11.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:18:18.47 Syringe tare is 51% of full scale at 22C (drifted 0%) @13:18:18.50 PinPort.in :locking @13:18:18.61 PinPort.seek :locked @13:18:19.17 Archive.in :fast @13:18:19.27 Lysis1.seek 1100uL @13:18:19.34 Lysis2.in :fast @13:18:19.50 Archive.seek :full @13:18:19.70 Lysis2.seek 1100uL @13:18:24.01 delayUntil "13:28:11.12" @13:28:11.13 Lysis2.in :DAmix @13:28:11.28 Lysis1.in :DAmix @13:28:11.84 Lysis2.seek 950uL @13:28:11.90 Lysis1.seek 950uL @13:28:32.80 delay 5 seconds @13:28:37.82 Handoff.select SPR @13:28:37.99 SPR.command "start 2 14" @13:28:39.16 SPR stabilized @13:28:39.35 delay 10 seconds @13:28:49.37 SPR.command "start 2 0" @13:28:52.53 Waiting up to 11:00 for SPR to enter state 1 @13:30:50.62 SPR.command "hold_on" @13:30:52.04 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @13:30:52.67 Lysis2.seek 500uL @13:30:52.73 Lysis1.seek 500uL @13:31:54.95 delay 15 seconds @13:32:10.00 SPR.command "hold_off" @13:32:13.50 Waiting up to 1:00:00 for SPR to enter state 7 @13:48:29.05 SPRsummary:-2.89RIU,132RIU,270RIU,5.65RIU after 1921s @14:02:46.11 delay 10 seconds @14:02:56.13 SPR.command "start 2 0" @14:02:58.82 Waiting up to 11:00 for SPR to enter state 1 @14:04:56.47 SPR.command "hold_on" @14:04:57.87 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:04:58.95 Lysis2.seek 275uL @14:04:58.99 Lysis1.seek 275uL @14:05:30.16 delay 15 seconds @14:05:45.18 SPR.command "hold_off" @14:05:48.63 Waiting up to 1:00:00 for SPR to enter state 7 @14:36:21.67 delay 10 seconds @14:36:31.70 SPR.command "start 2 0" @14:36:34.91 Waiting up to 11:00 for SPR to enter state 1 @14:38:33.01 SPR.command "hold_on" @14:38:34.39 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:38:35.09 Lysis2.seek 50uL @14:38:35.18 Lysis1.seek 50uL @14:39:06.38 delay 15 seconds @14:39:21.41 SPR.command "hold_off" @14:39:24.64 Waiting up to 1:00:00 for SPR to enter state 7 @15:09:57.19 Additional SPR Analyses Skipped due to Insufficient Volume @15:09:57.47 Handoff.select :close @15:09:57.66
Gate.power :heat,:OFF @15:09:57.68 Gate.power :cartridge,:OFF @15:09:57.81 PinPort.in :unlocking @15:09:57.88 PinPort.seek :clear @15:09:58.39 Carousel.in :default @15:09:58.54 Carousel.home.seek 57 @15:09:58.89 Plunge.seek :locked @15:09:59.14 Archive.in :unlocking @15:09:59.28 Archive.seek :clear @15:09:59.45 Air.in :unlocking @15:09:59.62 Air.seek :clear @15:09:59.75 Lysis1.in :unlocking @15:09:59.90 Lysis1.seek :clear @15:10:00.06 Lysis2.in :unlocking @15:10:00.17 Lysis2.seek :clear @15:10:21.09 Ended log spr24sep1913h.spr.bz2 @15:10:21.11
SPR.command "stats_off" @15:10:21.42 Gate.power :analytic1,:OFF @15:10:21.46 Gate.power :motor,:OFF @15:10:21.49 Gate.power :core,:OFF @15:10:21.53 Gate.power :main,:OFF @15:10:21.56 Safely stopped and ready to power off @15:10:22.34 Gate.power :sampler,:OFF,limit to 3500mA @15:10:22.36 Gate.power :skimmer,:OFF,limit to 3500mA @15:10:25.34 Gate.power :main,:ON,limit to 5000mA @15:10:25.36 Gate.power :analytic1,:ON,limit to 4000mA @15:10:25.52 Powering core bus @15:10:27.88 SPR Module 1, FW Rev. B, FPGA Rev. C @15:10:28.33
Plunge.configure :default @15:10:28.44 SamplePump.configure :default @15:10:28.56 Toroid.configure :default @15:10:28.67 Carousel.configure :default @15:10:28.78 Abs.configure :default @15:10:28.89 PhaseSeparator.configure :default @15:10:28.99 Archive.configure :default @15:10:29.10 Air.configure :default @15:10:29.18 Lysis1.configure :default @15:10:29.26 Lysis2.configure :default @15:10:29.37 PinPort.configure :default @15:10:29.49 All elf controllers running firmware version 4.92 @15:10:29.51 Gate.power :motor,:ON,limit to 5000mA @15:10:29.86 Plunge.home.jog 0 @15:10:30.54 Lysis2.home.jog 0 @15:10:30.61 Lysis1.home.jog 0 @15:10:30.64 Archive.home.jog 0 @15:10:30.68 Toroid.home.jog 0 @15:10:30.79 Carousel.home.jog 0 @15:10:30.86 PinPort.home.jog 0 @15:10:30.90 Air.home.jog 0 @15:10:31.85 Plunge.in :homing @15:10:32.19 Plunge.jog 1602 @15:10:32.28 Lysis2.in :homing @15:10:32.32 Air.in :homing @15:10:32.38 Toroid.in :homing @15:10:32.42 PinPort.in :homing @15:10:32.47 Archive.in :homing @15:10:32.51 Lysis1.in :homing @15:10:32.80 Lysis2.jog 1404 @15:10:32.96 Lysis1.jog 1397 @15:10:33.03 Archive.jog 1400 @15:10:33.09 PinPort.jog -111 @15:10:33.14 Toroid.jog 624 @15:10:33.19 Air.jog 1377 @15:10:33.73 PinPort.setRawPosition! 0 @15:10:33.82 PinPort.seek :home @15:10:33.95 Plunge.setRawPosition! 50 @15:10:34.03 Plunge.seek :home @15:10:34.15 PinPort.in :default @15:10:34.32 Plunge.in :default @15:10:34.46 Lysis2.setRawPosition! 50 @15:10:34.56 Lysis1.setRawPosition! 50 @15:10:34.60 Lysis2.seek :home @15:10:34.79 Lysis1.seek :home @15:10:34.93 Air.setRawPosition! 50 @15:10:34.96 Archive.setRawPosition! 50 @15:10:35.55 Air.seek :home @15:10:35.62 Archive.seek :home @15:10:35.97 Lysis2.in :default @15:10:35.99 Lysis1.in :default @15:10:36.04 Toroid.setRawPosition! 0 @15:10:36.23 Toroid.seek :home @15:10:36.38 Archive.in :default @15:10:36.47 Air.in :default @15:10:36.67 Toroid.in :default @15:10:37.22 Toroid.seek :clear @15:10:39.34
Carousel.in :default @15:10:39.52 Carousel.home.seek 57 @15:10:39.80 Selecting Cartridge 56 @15:10:40.12 Carousel.seek 56 @15:10:40.68 Lysis1.in :fast @15:10:40.76 Lysis1.seek :locked @15:10:43.44 Plunge.seek :locked @15:10:43.64 Air.in :fast @15:10:43.72 Air.seek :locked @15:10:44.72
Carousel.reconfigure :locked @15:10:44.90 Powering cartridge bus @15:10:47.57 Gate.setIR 1750,5000,0,6 @15:10:47.72 Gate.setIR 0,5000,0,6 @15:10:47.81 PuckHeater.configure :default @15:10:47.91 Gate.power :heat,:ON,limit to 5000mA @15:10:48.60 Mounted lysis Cartridge board#Q12558 @15:10:48.66 WARNING: Using default puck temperature sensor calibration @15:10:48.68 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:10:50.29 SPR.command "read_sequences" @15:10:50.42 SPR.command "stats_on" @15:10:50.59 SPR.command "start 0 1" @15:10:51.30 SPR.start /var/log/esp/spr24sep1915h.spr.bz2 @15:10:53.27 Waiting for baseline SPR stabilization @15:10:53.30 Handoff.select :waste @15:10:53.53 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:11:00.17 Syringe tare is 51% of full scale at 22C (drifted 0%) @15:11:00.20 PinPort.in :locking @15:11:00.30 PinPort.seek :locked @15:11:00.85 Archive.in :fast @15:11:00.96 Lysis2.in :fast @15:11:01.03 Lysis1.seek 1100uL @15:11:01.09 Archive.seek :full @15:11:01.25 Lysis2.seek 1100uL @15:11:05.59 delayUntil "15:20:52.83" @15:20:52.85 Lysis2.in :DAmix @15:20:52.99 Lysis1.in :DAmix @15:20:53.57 Lysis2.seek 950uL @15:20:53.60 Lysis1.seek 950uL @15:21:14.49 delay 5 seconds @15:21:19.52 Handoff.select SPR @15:21:19.68 SPR.command "start 2 14" @15:21:20.36 SPR stabilized @15:21:20.54 delay 10 seconds @15:21:30.56 SPR.command "start 2 0" @15:21:34.24 Waiting up to 11:00 for SPR to enter state 1 @15:23:32.34 SPR.command "hold_on" @15:23:33.73 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:23:34.39 Lysis2.seek 500uL @15:23:34.44 Lysis1.seek 500uL @15:24:36.69 delay 15 seconds @15:24:51.73 SPR.command "hold_off" @15:24:55.19 Waiting up to 1:00:00 for SPR to enter state 7 @15:41:10.76 SPRsummary:-3.19RIU,121RIU,273RIU,1.65RIU after 1921s @15:55:27.79 delay 10 seconds @15:55:37.82 SPR.command "start 2 0" @15:55:40.52 Waiting up to 11:00 for SPR to enter state 1 @15:57:38.18 SPR.command "hold_on" @15:57:40.08 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @15:57:40.77 Lysis2.seek 275uL @15:57:40.82 Lysis1.seek 275uL @15:58:12.04 delay 15 seconds @15:58:27.06 SPR.command "hold_off" @15:58:29.82 Waiting up to 1:00:00 for SPR to enter state 7 @16:29:01.50 delay 10 seconds @16:29:11.53 SPR.command "start 2 0" @16:29:14.19 Waiting up to 11:00 for SPR to enter state 1 @16:31:11.85 SPR.command "hold_on" @16:31:13.24 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:31:13.97 Lysis2.seek 50uL @16:31:14.01 Lysis1.seek 50uL @16:31:45.21 delay 15 seconds @16:32:00.23 SPR.command "hold_off" @16:32:04.00 Waiting up to 1:00:00 for SPR to enter state 7 @16:35:45.23 Session Begun @16:35:49.76 -> showlog -100 @16:49:49.69 Session Ended @16:58:37.95 Session Ended @17:02:36.98 Additional SPR Analyses Skipped due to Insufficient Volume @17:02:37.19 Handoff.select :close @17:02:37.35
Gate.power :heat,:OFF @17:02:37.37 Gate.power :cartridge,:OFF @17:02:37.48 PinPort.in :unlocking @17:02:37.53 PinPort.seek :clear @17:02:38.01 Carousel.in :default @17:02:38.13 Carousel.home.seek 56 @17:02:38.45 Plunge.seek :locked @17:02:38.63 Archive.in :unlocking @17:02:38.77 Archive.seek :clear @17:02:38.83 Air.in :unlocking @17:02:39.01 Air.seek :clear @17:02:39.13 Lysis1.in :unlocking @17:02:39.25 Lysis1.seek :clear @17:02:39.37 Lysis2.in :unlocking @17:02:39.47 Lysis2.seek :clear @17:03:00.11 Ended log spr24sep1915h.spr.bz2 @17:03:00.13
SPR.command "stats_off" @17:03:00.37 Gate.power :analytic1,:OFF @17:03:00.38 Gate.power :motor,:OFF @17:03:00.39 Gate.power :core,:OFF @17:03:00.41 Gate.power :main,:OFF @17:03:00.42 Safely stopped and ready to power off @17:03:01.12 Gate.power :sampler,:OFF,limit to 3500mA @17:03:01.13 Gate.power :skimmer,:OFF,limit to 3500mA @17:03:04.22 Gate.power :main,:ON,limit to 5000mA @17:03:04.23 Gate.power :analytic1,:ON,limit to 4000mA @17:03:04.30 Powering core bus @17:03:06.67 SPR Module 1, FW Rev. B, FPGA Rev. C @17:03:07.66
Plunge.configure :default @17:03:07.74 SamplePump.configure :default @17:03:07.82 Toroid.configure :default @17:03:07.90 Carousel.configure :default @17:03:07.97 Abs.configure :default @17:03:08.05 PhaseSeparator.configure :default @17:03:08.13 Archive.configure :default @17:03:08.21 Air.configure :default @17:03:08.26 Lysis1.configure :default @17:03:08.32 Lysis2.configure :default @17:03:08.39 PinPort.configure :default @17:03:08.44 All elf controllers running firmware version 4.92 @17:03:08.47 Gate.power :motor,:ON,limit to 5000mA @17:03:08.79 Plunge.home.jog 0 @17:03:09.43 Lysis2.home.jog 0 @17:03:09.51 Air.home.jog 0 @17:03:09.54 Carousel.home.jog 0 @17:03:09.61 Archive.home.jog 0 @17:03:09.66 Toroid.home.jog 0 @17:03:09.69 Lysis1.home.jog 0 @17:03:09.72 PinPort.home.jog 0 @17:03:10.51 Plunge.in :homing @17:03:10.78 Plunge.jog 1600 @17:03:10.97 Lysis2.in :homing @17:03:11.03 Lysis1.in :homing @17:03:11.07 Archive.in :homing @17:03:11.08 PinPort.in :homing @17:03:11.12 Air.in :homing @17:03:11.14 Toroid.in :homing @17:03:11.38 Lysis1.jog 1399 @17:03:11.46 PinPort.jog -112 @17:03:11.51 Lysis2.jog 1406 @17:03:11.53 Archive.jog 1398 @17:03:11.57 Air.jog 1377 @17:03:11.62 Toroid.jog 627 @17:03:12.06 Plunge.setRawPosition! 50 @17:03:12.08 PinPort.setRawPosition! 0 @17:03:12.19 PinPort.seek :home @17:03:12.21 Plunge.seek :home @17:03:12.41 PinPort.in :default @17:03:12.58 Plunge.in :default @17:03:12.79 Lysis1.setRawPosition! 50 @17:03:12.84 Lysis1.seek :home @17:03:12.93 Lysis2.setRawPosition! 50 @17:03:12.98 Lysis2.seek :home @17:03:13.12 Lysis1.in :default @17:03:13.15 Air.setRawPosition! 50 @17:03:13.17 Archive.setRawPosition! 50 @17:03:13.32 Air.seek :home @17:03:13.36 Archive.seek :home @17:03:13.56 Lysis2.in :default @17:03:13.70 Air.in :default @17:03:13.79 Archive.in :default @17:03:14.03 Toroid.setRawPosition! 0 @17:03:14.09 Toroid.seek :home @17:03:14.19 Toroid.in :default @17:03:14.69 Toroid.seek :clear @17:03:16.44
Carousel.in :default @17:03:16.56 Carousel.home.seek 56 @17:03:16.83 Selecting Cartridge 55 @17:03:17.11 Carousel.seek 55 @17:03:17.64 Lysis1.in :fast @17:03:17.69 Lysis1.seek :locked @17:03:20.31 Plunge.seek :locked @17:03:20.47 Air.in :fast @17:03:20.53 Air.seek :locked @17:03:21.50
Carousel.reconfigure :locked @17:03:21.65 Powering cartridge bus @17:03:24.30 Gate.setIR 1750,5000,0,6 @17:03:24.42 Gate.setIR 0,5000,0,6 @17:03:24.49 PuckHeater.configure :default @17:03:24.55 Gate.power :heat,:ON,limit to 5000mA @17:03:25.16 Mounted lysis Cartridge @17:03:25.19 WARNING: Using default puck temperature sensor calibration @17:03:25.21 Using default cartridge heat loss curve for HeatingAccelInCarousel /home/esp/esp2/lib/thread.rb:984: [BUG] Segmentation fault ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:15:29.35PDT23-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @11:15:31.39 >BREAK< ESP3 Gateway v1.43 for core bus @11:15:31.42 >BREAK< ESP3 Gateway v1.43 for cartridge bus @11:15:34.93 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @11:15:35.61 Sea pressure (Abs) tare is 6% of full scale @11:15:39.26 Polling Can every 2:00 @11:15:39.31 Loading /home/esp/esp2/bin/processAll mission @11:15:39.53 Sea pressure (Abs) tare is 6% of full scale @11:15:39.55 Sampling default goal volumes @11:15:39.57 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @11:15:39.73 Gate.power :sampler,:OFF,limit to 3500mA @11:15:39.74 Gate.power :skimmer,:OFF,limit to 3500mA @11:15:39.76 Gate.power :analytic1,:ON,limit to 4000mA @11:15:40.42 Session Begun @11:15:40.63 SPR Module 1, FW Rev. B, FPGA Rev. C @11:15:41.03
Plunge.configure :default @11:15:41.21 SamplePump.configure :default @11:15:41.37 Toroid.configure :default @11:15:41.45 Toroid.hold @11:15:41.55 Toroid.configure :default @11:15:41.71 Carousel.configure :default @11:15:41.78 Carousel.hold @11:15:41.88 Carousel.configure :default @11:15:42.07 Abs.configure :default @11:15:42.24 PhaseSeparator.configure :default @11:15:42.41 Archive.configure :default @11:15:42.57 Air.configure :default @11:15:42.71 Lysis1.configure :default @11:15:42.85 Lysis2.configure :default @11:15:43.02 PinPort.configure :default @11:15:43.10 All elf controllers running firmware version 4.92 @11:15:43.93 Toroid.seek :clear @11:15:44.16
Carousel.in :default @11:15:44.30 Carousel.home.seek 55 @11:15:44.60 Selecting Cartridge 55 @11:15:44.92 Carousel.seek 55 @11:15:45.05 Gate.power :heat,:OFF @11:15:45.08 Gate.power :cartridge,:OFF @11:15:45.29 Lysis1.in :fast @11:15:45.37 Lysis1.seek :locked @11:15:48.12 Plunge.seek :locked @11:15:48.32 Air.in :fast @11:15:48.40 Air.seek :locked @11:15:49.40
Carousel.reconfigure :locked @11:15:49.58 Powering cartridge bus @11:15:53.23 Gate.setIR 1750,5000,0,6 @11:15:53.36 Gate.setIR 0,5000,0,6 @11:15:53.45 PuckHeater.configure :default @11:15:53.54 Gate.power :heat,:ON,limit to 5000mA @11:15:54.21 Mounted lysis Cartridge @11:15:54.25 WARNING: Using default puck temperature sensor calibration @11:15:54.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:15:55.88 SPR.command "read_sequences" @11:15:56.50 SPR.command "stats_on" @11:15:56.68 SPR.command "start 0 1" @11:15:57.39 SPR.start /var/log/esp/spr24sep2311h.spr.bz2 @11:15:59.36 Waiting for baseline SPR stabilization @11:15:59.38 Handoff.select :waste @11:15:59.56 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:16:06.21 Syringe tare is 52% of full scale at 22C (drifted 1%) @11:16:06.27 PinPort.in :locking @11:16:06.35 PinPort.seek :locked @11:16:06.87 Archive.in :fast @11:16:06.96 Lysis2.in :fast @11:16:07.06 Archive.seek :full @11:16:07.12 Lysis1.seek 1100uL @11:16:07.25 Lysis2.seek 1100uL @11:16:11.56 delayUntil "11:25:58.91" @11:22:23.13 Session Begun @11:25:58.92 Lysis2.in :DAmix @11:25:59.09 Lysis1.in :DAmix @11:25:59.70 Lysis2.seek 950uL @11:25:59.81 Lysis1.seek 950uL @11:26:20.64 delay 5 seconds @11:26:25.67 Handoff.select SPR @11:26:25.85 SPR.command "start 2 14" @11:26:26.44 SPR stabilized @11:26:26.65 delay 10 seconds @11:26:36.67 SPR.command "start 2 0" @11:26:39.81 Waiting up to 11:00 for SPR to enter state 1 @11:28:37.48 SPR.command "hold_on" @11:28:38.87 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @11:28:39.53 Lysis2.seek 500uL @11:28:39.59 Lysis1.seek 500uL @11:29:41.80 delay 15 seconds @11:29:56.84 SPR.command "hold_off" @11:30:00.33 Waiting up to 1:00:00 for SPR to enter state 7 @11:46:15.89 SPRsummary:44.6RIU,176RIU,351RIU,78.4RIU after 1920s @12:00:32.93 delay 10 seconds @12:00:42.96 SPR.command "start 2 0" @12:00:46.17 Waiting up to 11:00 for SPR to enter state 1 @12:02:44.27 SPR.command "hold_on" @12:02:45.65 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:02:46.73 Lysis2.seek 275uL @12:02:46.84 Lysis1.seek 275uL @12:03:18.02 delay 15 seconds @12:03:33.05 SPR.command "hold_off" @12:03:35.90 Waiting up to 1:00:00 for SPR to enter state 7 @12:34:08.51 delay 10 seconds @12:34:18.53 SPR.command "start 2 0" @12:34:21.22 Waiting up to 11:00 for SPR to enter state 1 @12:36:18.87 SPR.command "hold_on" @12:36:20.27 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:36:20.96 Lysis2.seek 50uL @12:36:21.07 Lysis1.seek 50uL @12:36:52.23 delay 15 seconds @12:37:07.25 SPR.command "hold_off" @12:37:10.52 Waiting up to 1:00:00 for SPR to enter state 7 @13:07:43.05 Additional SPR Analyses Skipped due to Insufficient Volume @13:07:43.30 Intake.close @13:07:43.37 Exhaust.close @13:07:43.45 Bypass.close @13:07:43.63 Handoff.select :close @13:07:43.99
Gate.power :heat,:OFF @13:07:44.04 Gate.power :cartridge,:OFF @13:07:44.22 PinPort.in :unlocking @13:07:44.32 PinPort.seek :clear @13:07:44.86 Carousel.in :default @13:07:45.07 Carousel.home.seek 55 @13:07:45.44 Plunge.seek :locked @13:07:45.68 Archive.in :unlocking @13:07:45.89 Archive.seek :clear @13:07:46.09 Air.in :unlocking @13:07:46.27 Air.seek :clear @13:07:46.44 Lysis1.in :unlocking @13:07:46.62 Lysis1.seek :clear @13:07:46.81 Lysis2.in :unlocking @13:07:46.93 Lysis2.seek :clear @13:08:07.69 Ended log spr24sep2311h.spr.bz2 @13:08:07.71
SPR.command "stats_off" @13:08:08.18 Gate.power :analytic1,:OFF @13:08:08.21 Gate.power :motor,:OFF @13:08:08.26 Gate.power :core,:OFF @13:08:08.31 Gate.power :main,:OFF @13:08:08.34 Safely stopped and ready to power off @13:08:09.16 Gate.power :sampler,:OFF,limit to 3500mA @13:08:09.18 Gate.power :skimmer,:OFF,limit to 3500mA @13:08:12.12 Gate.power :main,:ON,limit to 5000mA @13:08:12.14 Gate.power :analytic1,:ON,limit to 4000mA @13:08:12.29 Powering core bus @13:08:14.61 SPR Module 1, FW Rev. B, FPGA Rev. C @13:08:15.07
Plunge.configure :default @13:08:15.75 SamplePump.configure :default @13:08:15.88 Toroid.configure :default @13:08:16.03 Carousel.configure :default @13:08:16.15 Abs.configure :default @13:08:16.28 PhaseSeparator.configure :default @13:08:16.40 Archive.configure :default @13:08:16.52 Air.configure :default @13:08:16.63 Lysis1.configure :default @13:08:16.73 Lysis2.configure :default @13:08:16.85 PinPort.configure :default @13:08:16.95 All elf controllers running firmware version 4.92 @13:08:16.98 Gate.power :motor,:ON,limit to 5000mA @13:08:17.25 Plunge.home.jog 0 @13:08:18.08 PinPort.home.jog 0 @13:08:18.13 Lysis1.home.jog 0 @13:08:18.20 Archive.home.jog 0 @13:08:18.29 Toroid.home.jog 0 @13:08:18.35 Lysis2.home.jog 0 @13:08:18.42 Carousel.home.jog 0 @13:08:18.47 Air.home.jog 0 @13:08:19.27 Plunge.in :homing @13:08:19.69 Plunge.jog 1601 @13:08:19.93 Lysis2.in :homing @13:08:20.01 Lysis1.in :homing @13:08:20.07 Archive.in :homing @13:08:20.15 PinPort.in :homing @13:08:20.20 Air.in :homing @13:08:20.27 Toroid.in :homing @13:08:20.52 Lysis2.jog 1406 @13:08:20.71 Lysis1.jog 1399 @13:08:20.75 Air.jog 1380 @13:08:20.83 Archive.jog 1399 @13:08:20.88 PinPort.jog -112 @13:08:20.97 Toroid.jog 626 @13:08:21.47 Plunge.setRawPosition! 50 @13:08:21.58 Plunge.seek :home @13:08:21.72 PinPort.setRawPosition! 0 @13:08:21.82 PinPort.seek :home @13:08:21.99 PinPort.in :default @13:08:22.03 Plunge.in :default @13:08:22.28 Lysis2.setRawPosition! 50 @13:08:22.39 Lysis2.seek :home @13:08:22.57 Lysis1.setRawPosition! 50 @13:08:22.66 Air.setRawPosition! 50 @13:08:22.74 Archive.setRawPosition! 50 @13:08:22.84 Lysis1.seek :home @13:08:22.96 Air.seek :home @13:08:23.06 Archive.seek :home @13:08:23.37 Lysis1.in :default @13:08:23.40 Lysis2.in :default @13:08:23.65 Archive.in :default @13:08:23.69 Toroid.setRawPosition! 0 @13:08:23.75 Air.in :default @13:08:23.97 Toroid.seek :home @13:08:24.16 Toroid.in :default @13:08:24.34 Toroid.seek :clear @13:08:26.10
Carousel.in :default @13:08:26.68 Carousel.home.seek 55 @13:08:27.10 Selecting Cartridge 54 @13:08:27.48 Carousel.seek 54 @13:08:28.08 Lysis1.in :fast @13:08:28.17 Lysis1.seek :locked @13:08:30.85 Plunge.seek :locked @13:08:31.06 Air.in :fast @13:08:31.17 Air.seek :locked @13:08:32.21
Carousel.reconfigure :locked @13:08:32.44 Powering cartridge bus @13:08:35.10 Gate.setIR 1750,5000,0,6 @13:08:35.23 Gate.setIR 0,5000,0,6 @13:08:35.35 PuckHeater.configure :default @13:08:35.46 Gate.power :heat,:ON,limit to 5000mA @13:08:36.12 Mounted lysis Cartridge @13:08:36.21 WARNING: Using default puck temperature sensor calibration @13:08:36.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:08:37.89 SPR.command "read_sequences" @13:08:38.16 SPR.command "stats_on" @13:08:38.35 SPR.command "start 0 1" @13:08:39.06 SPR.start /var/log/esp/spr24sep2313h.spr.bz2 @13:08:41.53 Waiting for baseline SPR stabilization @13:08:41.55 Handoff.select :waste @13:08:41.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:08:48.41 Syringe tare is 52% of full scale at 22C (drifted 1%) @13:08:48.48 PinPort.in :locking @13:08:48.59 PinPort.seek :locked @13:08:49.14 Archive.in :fast @13:08:49.27 Lysis2.in :fast @13:08:49.37 Lysis1.seek 1100uL @13:08:49.49 Archive.seek :full @13:08:49.58 Lysis2.seek 1100uL @13:08:54.59 delayUntil "13:18:41.49" @13:18:41.51 Lysis2.in :DAmix @13:18:41.67 Lysis1.in :DAmix @13:18:42.36 Lysis2.seek 950uL @13:18:42.43 Lysis1.seek 950uL @13:19:03.35 delay 5 seconds @13:19:08.38 Handoff.select SPR @13:19:08.57 SPR.command "start 2 14" @13:19:10.04 SPR stabilized @13:19:10.24 delay 10 seconds @13:19:20.27 SPR.command "start 2 0" @13:19:23.36 Waiting up to 11:00 for SPR to enter state 1 @13:21:21.03 SPR.command "hold_on" @13:21:22.93 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @13:21:23.59 Lysis2.seek 500uL @13:21:23.68 Lysis1.seek 500uL @13:22:25.92 delay 15 seconds @13:22:40.94 SPR.command "hold_off" @13:22:43.87 Waiting up to 1:00:00 for SPR to enter state 7 @13:38:58.49 SPRsummary:-2.66RIU,84.6RIU,179RIU,-0.527RIU after 1921s @13:53:15.52 delay 10 seconds @13:53:25.55 SPR.command "start 2 0" @13:53:28.25 Waiting up to 11:00 for SPR to enter state 1 @13:55:25.92 SPR.command "hold_on" @13:55:27.30 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:55:28.42 Lysis2.seek 275uL @13:55:28.53 Lysis1.seek 275uL @13:55:59.73 delay 15 seconds @13:56:14.75 SPR.command "hold_off" @13:56:17.55 Waiting up to 1:00:00 for SPR to enter state 7 @14:26:50.19 delay 10 seconds @14:27:00.21 SPR.command "start 2 0" @14:27:02.86 Waiting up to 11:00 for SPR to enter state 1 @14:29:00.52 SPR.command "hold_on" @14:29:01.92 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:29:02.67 Lysis2.seek 50uL @14:29:02.73 Lysis1.seek 50uL @14:29:33.98 delay 15 seconds @14:29:49.04 SPR.command "hold_off" @14:29:52.17 Waiting up to 1:00:00 for SPR to enter state 7 @15:00:24.71 Additional SPR Analyses Skipped due to Insufficient Volume @15:00:24.96 Handoff.select :close @15:00:25.16
Gate.power :heat,:OFF @15:00:25.21 Gate.power :cartridge,:OFF @15:00:25.34 PinPort.in :unlocking @15:00:25.44 PinPort.seek :clear @15:00:25.97 Carousel.in :default @15:00:26.17 Carousel.home.seek 54 @15:00:26.59 Plunge.seek :locked @15:00:26.83 Archive.in :unlocking @15:00:27.03 Archive.seek :clear @15:00:27.10 Air.in :unlocking @15:00:27.35 Air.seek :clear @15:00:27.50 Lysis1.in :unlocking @15:00:27.73 Lysis1.seek :clear @15:00:27.85 Lysis2.in :unlocking @15:00:28.04 Lysis2.seek :clear @15:00:48.37 Ended log spr24sep2313h.spr.bz2 @15:00:48.39
SPR.command "stats_off" @15:00:48.89 Gate.power :analytic1,:OFF @15:00:48.91 Gate.power :motor,:OFF @15:00:48.97 Gate.power :core,:OFF @15:00:49.02 Gate.power :main,:OFF @15:00:49.08 Safely stopped and ready to power off @15:00:49.39 Gate.power :sampler,:OFF,limit to 3500mA @15:00:49.41 Gate.power :skimmer,:OFF,limit to 3500mA @15:00:52.83 Gate.power :main,:ON,limit to 5000mA @15:00:52.85 Gate.power :analytic1,:ON,limit to 4000mA @15:00:53.00 Powering core bus @15:00:55.32 SPR Module 1, FW Rev. B, FPGA Rev. C @15:00:56.35
Plunge.configure :default @15:00:56.47 SamplePump.configure :default @15:00:56.60 Toroid.configure :default @15:00:56.73 Carousel.configure :default @15:00:56.86 Abs.configure :default @15:00:56.99 PhaseSeparator.configure :default @15:00:57.11 Archive.configure :default @15:00:57.25 Air.configure :default @15:00:57.34 Lysis1.configure :default @15:00:57.45 Lysis2.configure :default @15:00:57.58 PinPort.configure :default @15:00:57.67 All elf controllers running firmware version 4.92 @15:00:57.70 Gate.power :motor,:ON,limit to 5000mA @15:00:58.16 Plunge.home.jog 0 @15:00:58.82 PinPort.home.jog 0 @15:00:58.89 Lysis2.home.jog 0 @15:00:58.95 Air.home.jog 0 @15:00:59.03 Carousel.home.jog 0 @15:00:59.09 Archive.home.jog 0 @15:00:59.15 Toroid.home.jog 0 @15:00:59.22 Lysis1.home.jog 0 @15:01:00.09 Plunge.in :homing @15:01:00.46 Plunge.jog 1603 @15:01:00.70 Lysis2.in :homing @15:01:00.76 Lysis1.in :homing @15:01:00.82 Archive.in :homing @15:01:00.89 PinPort.in :homing @15:01:00.95 Air.in :homing @15:01:01.00 Toroid.in :homing @15:01:01.28 Lysis2.jog 1404 @15:01:01.49 PinPort.jog -111 @15:01:01.66 Lysis1.jog 1400 @15:01:01.76 Archive.jog 1401 @15:01:01.82 Toroid.jog 626 @15:01:01.88 Air.jog 1379 @15:01:02.37 PinPort.setRawPosition! 0 @15:01:02.39 Plunge.setRawPosition! 50 @15:01:02.57 PinPort.seek :home @15:01:02.61 Plunge.seek :home @15:01:02.91 PinPort.in :default @15:01:02.99 Lysis2.setRawPosition! 50 @15:01:03.12 Lysis2.seek :home @15:01:03.28 Plunge.in :default @15:01:03.44 Lysis1.setRawPosition! 50 @15:01:03.47 Air.setRawPosition! 50 @15:01:03.54 Archive.setRawPosition! 50 @15:01:03.76 Lysis1.seek :home @15:01:03.81 Air.seek :home @15:01:03.85 Archive.seek :home @15:01:04.25 Lysis2.in :default @15:01:04.47 Air.in :default @15:01:04.50 Archive.in :default @15:01:04.58 Toroid.setRawPosition! 0 @15:01:04.67 Lysis1.in :default @15:01:04.93 Toroid.seek :home @15:01:05.08 Toroid.in :default @15:01:05.75 Toroid.seek :clear @15:01:07.46
Carousel.in :default @15:01:07.62 Carousel.home.seek 54 @15:01:07.93 Selecting Cartridge 53 @15:01:08.33 Carousel.seek 53 @15:01:08.92 Lysis1.in :fast @15:01:09.02 Lysis1.seek :locked @15:01:11.69 Plunge.seek :locked @15:01:11.90 Air.in :fast @15:01:12.04 Air.seek :locked @15:01:13.06
Carousel.reconfigure :locked @15:01:13.26 Powering cartridge bus @15:01:15.92 Gate.setIR 1750,5000,0,6 @15:01:16.06 Gate.setIR 0,5000,0,6 @15:01:16.17 PuckHeater.configure :default @15:01:16.29 Gate.power :heat,:ON,limit to 5000mA @15:01:16.92 Mounted lysis Cartridge @15:01:16.97 WARNING: Using default puck temperature sensor calibration @15:01:17.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:01:18.65 SPR.command "read_sequences" @15:01:18.87 SPR.command "stats_on" @15:01:19.06 SPR.command "start 0 1" @15:01:19.77 SPR.start /var/log/esp/spr24sep2315h.spr.bz2 @15:01:21.72 Waiting for baseline SPR stabilization @15:01:21.74 Handoff.select :waste @15:01:21.95 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:01:28.59 Syringe tare is 52% of full scale at 22C (drifted 1%) @15:01:28.63 PinPort.in :locking @15:01:28.76 PinPort.seek :locked @15:01:29.32 Archive.in :fast @15:01:30.02 Lysis2.in :fast @15:01:30.14 Lysis1.seek 1100uL @15:01:30.22 Archive.seek :full @15:01:30.48 Lysis2.seek 1100uL @15:01:34.77 delayUntil "15:11:21.28" @15:11:21.30 Lysis2.in :DAmix @15:11:21.45 Lysis1.in :DAmix @15:11:22.16 Lysis2.seek 950uL @15:11:22.20 Lysis1.seek 950uL @15:11:43.14 delay 5 seconds @15:11:48.17 Handoff.select SPR @15:11:48.36 SPR.command "start 2 14" @15:11:50.27 SPR stabilized @15:11:50.49 delay 10 seconds @15:12:00.51 SPR.command "start 2 0" @15:12:03.64 Waiting up to 11:00 for SPR to enter state 1 @15:14:01.72 SPR.command "hold_on" @15:14:03.64 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:14:04.32 Lysis2.seek 500uL @15:14:04.36 Lysis1.seek 500uL @15:15:06.66 delay 15 seconds @15:15:21.68 SPR.command "hold_off" @15:15:24.58 Waiting up to 1:00:00 for SPR to enter state 7 @15:31:39.20 SPRsummary:0.513RIU,35.4RIU,66.7RIU,3.1RIU after 1921s @15:45:56.24 delay 10 seconds @15:46:06.26 SPR.command "start 2 0" @15:46:08.95 Waiting up to 11:00 for SPR to enter state 1 @15:48:06.61 SPR.command "hold_on" @15:48:08.04 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @15:48:08.73 Lysis2.seek 275uL @15:48:08.80 Lysis1.seek 275uL @15:48:40.06 delay 15 seconds @15:48:55.08 SPR.command "hold_off" @15:48:58.25 Waiting up to 1:00:00 for SPR to enter state 7 @16:19:30.87 delay 10 seconds @16:19:40.90 SPR.command "start 2 0" @16:19:43.58 Waiting up to 11:00 for SPR to enter state 1 @16:21:41.25 SPR.command "hold_on" @16:21:42.63 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:21:43.32 Lysis2.seek 50uL @16:21:43.36 Lysis1.seek 50uL @16:22:14.61 delay 15 seconds @16:22:29.63 SPR.command "hold_off" @16:22:32.88 Waiting up to 1:00:00 for SPR to enter state 7 @16:53:05.42 Additional SPR Analyses Skipped due to Insufficient Volume @16:53:05.68 Handoff.select :close @16:53:05.88
Gate.power :heat,:OFF @16:53:05.90 Gate.power :cartridge,:OFF @16:53:06.10 PinPort.in :unlocking @16:53:06.19 PinPort.seek :clear @16:53:06.74 Carousel.in :default @16:53:06.92 Carousel.home.seek 53 @16:53:07.35 Plunge.seek :locked @16:53:07.58 Archive.in :unlocking @16:53:07.75 Archive.seek :clear @16:53:07.89 Air.in :unlocking @16:53:08.10 Air.seek :clear @16:53:08.29 Lysis1.in :unlocking @16:53:08.46 Lysis1.seek :clear @16:53:08.63 Lysis2.in :unlocking @16:53:08.74 Lysis2.seek :clear @16:53:29.02 Ended log spr24sep2315h.spr.bz2 @16:53:29.05
SPR.command "stats_off" @16:53:29.56 Gate.power :analytic1,:OFF @16:53:29.58 Gate.power :motor,:OFF @16:53:29.64 Gate.power :core,:OFF @16:53:29.70 Gate.power :main,:OFF @16:53:29.75 Safely stopped and ready to power off @16:53:30.06 Gate.power :sampler,:OFF,limit to 3500mA @16:53:30.09 Gate.power :skimmer,:OFF,limit to 3500mA @16:53:33.51 Gate.power :main,:ON,limit to 5000mA @16:53:33.53 Gate.power :analytic1,:ON,limit to 4000mA @16:53:33.68 Powering core bus @16:53:36.05 SPR Module 1, FW Rev. B, FPGA Rev. C @16:53:36.47
Plunge.configure :default @16:53:36.59 SamplePump.configure :default @16:53:36.71 Toroid.configure :default @16:53:36.84 Carousel.configure :default @16:53:36.96 Abs.configure :default @16:53:37.08 PhaseSeparator.configure :default @16:53:37.20 Archive.configure :default @16:53:37.32 Air.configure :default @16:53:37.41 Lysis1.configure :default @16:53:37.50 Lysis2.configure :default @16:53:37.63 PinPort.configure :default @16:53:37.72 All elf controllers running firmware version 4.92 @16:53:37.76 Gate.power :motor,:ON,limit to 5000mA @16:53:38.18 Plunge.home.jog 0 @16:53:39.42 Lysis2.home.jog 0 @16:53:39.46 Lysis1.home.jog 0 @16:53:39.53 Air.home.jog 0 @16:53:39.59 Carousel.home.jog 0 @16:53:39.68 PinPort.home.jog 0 @16:53:39.75 Archive.home.jog 0 @16:53:39.82 Toroid.home.jog 0 @16:53:40.75 Plunge.in :homing @16:53:41.10 Plunge.jog 1597 @16:53:41.40 Toroid.in :homing @16:53:41.49 Lysis2.in :homing @16:53:41.57 Air.in :homing @16:53:41.63 Lysis1.in :homing @16:53:41.69 PinPort.in :homing @16:53:41.77 Archive.in :homing @16:53:42.08 Toroid.jog 630 @16:53:42.19 Lysis2.jog 1405 @16:53:42.26 Air.jog 1382 @16:53:42.32 Lysis1.jog 1399 @16:53:42.39 PinPort.jog -111 @16:53:42.53 Archive.jog 1400 @16:53:42.94 Plunge.setRawPosition! 50 @16:53:43.05 Plunge.seek :home @16:53:43.20 PinPort.setRawPosition! 0 @16:53:43.29 PinPort.seek :home @16:53:43.43 Plunge.in :default @16:53:43.55 PinPort.in :default @16:53:43.72 Lysis2.setRawPosition! 50 @16:53:43.84 Air.setRawPosition! 50 @16:53:43.88 Lysis2.seek :home @16:53:44.13 Air.seek :home @16:53:44.28 Lysis1.setRawPosition! 50 @16:53:44.32 Archive.setRawPosition! 50 @16:53:44.53 Lysis1.seek :home @16:53:44.56 Archive.seek :home @16:53:44.84 Lysis2.in :default @16:53:44.85 Air.in :default @16:53:44.93 Toroid.setRawPosition! 0 @16:53:45.14 Toroid.seek :home @16:53:45.28 Archive.in :default @16:53:45.33 Lysis1.in :default @16:53:45.54 Toroid.in :default @16:53:45.77 Toroid.seek :clear @16:53:47.52
Carousel.in :default @16:53:47.68 Carousel.home.seek 53 @16:53:48.00 Selecting Cartridge 52 @16:53:48.36 Carousel.seek 52 @16:53:48.93 Lysis1.in :fast @16:53:49.03 Lysis1.seek :locked @16:53:52.23 Plunge.seek :locked @16:53:52.46 Air.in :fast @16:53:52.55 Air.seek :locked @16:53:53.58
Carousel.reconfigure :locked @16:53:53.78 Powering cartridge bus @16:53:56.44 Gate.setIR 1750,5000,0,6 @16:53:56.57 Gate.setIR 0,5000,0,6 @16:53:56.68 PuckHeater.configure :default @16:53:56.79 Gate.power :heat,:ON,limit to 5000mA @16:53:57.44 Mounted lysis Cartridge @16:53:57.49 WARNING: Using default puck temperature sensor calibration @16:53:57.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:53:59.23 SPR.command "read_sequences" @16:53:59.55 SPR.command "stats_on" @16:53:59.74 SPR.command "start 0 1" @16:54:00.45 SPR.start /var/log/esp/spr24sep2316h.spr.bz2 @16:54:02.40 Waiting for baseline SPR stabilization @16:54:02.42 Handoff.select :waste @16:54:02.65 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:54:09.29 Syringe tare is 52% of full scale at 22C (drifted 1%) @16:54:09.33 PinPort.in :locking @16:54:09.45 PinPort.seek :locked @16:54:10.06 Archive.in :fast @16:54:10.18 Lysis2.in :fast @16:54:10.27 Lysis1.seek 1100uL @16:54:10.34 Archive.seek :full @16:54:10.59 Lysis2.seek 1100uL @16:54:14.93 delayUntil "17:04:01.96" @17:04:01.98 Lysis2.in :DAmix @17:04:02.17 Lysis1.in :DAmix @17:04:02.81 Lysis2.seek 950uL @17:04:02.86 Lysis1.seek 950uL @17:04:23.80 delay 5 seconds @17:04:28.82 Handoff.select SPR @17:04:29.01 SPR.command "start 2 14" @17:04:30.43 SPR stabilized @17:04:30.63 delay 10 seconds @17:04:40.66 SPR.command "start 2 0" @17:04:43.80 Waiting up to 11:00 for SPR to enter state 1 @17:06:41.47 SPR.command "hold_on" @17:06:43.37 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @17:06:44.04 Lysis2.seek 500uL @17:06:44.12 Lysis1.seek 500uL @17:07:46.40 delay 15 seconds @17:08:01.42 SPR.command "hold_off" @17:08:04.83 Waiting up to 1:00:00 for SPR to enter state 7 @17:24:19.88 SPRsummary:0.665RIU,20.5RIU,44.4RIU,1.22RIU after 1921s @17:38:36.92 delay 10 seconds @17:38:46.95 SPR.command "start 2 0" @17:38:49.62 Waiting up to 11:00 for SPR to enter state 1 @17:40:47.30 SPR.command "hold_on" @17:40:48.69 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @17:40:49.44 Lysis2.seek 275uL @17:40:49.48 Lysis1.seek 275uL @17:41:20.72 delay 15 seconds @17:41:35.74 SPR.command "hold_off" @17:41:38.94 Waiting up to 1:00:00 for SPR to enter state 7 @18:12:11.54 delay 10 seconds @18:12:21.56 SPR.command "start 2 0" @18:12:24.26 Waiting up to 11:00 for SPR to enter state 1 @18:14:21.92 SPR.command "hold_on" @18:14:23.31 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @18:14:24.05 Lysis2.seek 50uL @18:14:24.10 Lysis1.seek 50uL @18:14:55.36 delay 15 seconds @18:15:10.39 SPR.command "hold_off" @18:15:13.56 Waiting up to 1:00:00 for SPR to enter state 7 @18:45:46.11 Additional SPR Analyses Skipped due to Insufficient Volume @18:45:46.38 Handoff.select :close @18:45:46.58
Gate.power :heat,:OFF @18:45:46.61 Gate.power :cartridge,:OFF @18:45:46.77 PinPort.in :unlocking @18:45:46.86 PinPort.seek :clear @18:45:47.41 Carousel.in :default @18:45:47.57 Carousel.home.seek 52 @18:45:48.03 Plunge.seek :locked @18:45:48.29 Archive.in :unlocking @18:45:48.48 Archive.seek :clear @18:45:48.61 Air.in :unlocking @18:45:48.82 Air.seek :clear @18:45:48.90 Lysis1.in :unlocking @18:45:49.15 Lysis1.seek :clear @18:45:49.31 Lysis2.in :unlocking @18:45:49.42 Lysis2.seek :clear @18:46:09.62 Ended log spr24sep2316h.spr.bz2 @18:46:09.65
SPR.command "stats_off" @18:46:10.24 Gate.power :analytic1,:OFF @18:46:10.26 Gate.power :motor,:OFF @18:46:10.34 Gate.power :core,:OFF @18:46:10.36 Gate.power :main,:OFF @18:46:10.39 Safely stopped and ready to power off @18:46:10.64 Gate.power :sampler,:OFF,limit to 3500mA @18:46:10.67 Gate.power :skimmer,:OFF,limit to 3500mA @18:46:14.17 Gate.power :main,:ON,limit to 5000mA @18:46:14.19 Gate.power :analytic1,:ON,limit to 4000mA @18:46:14.33 Powering core bus @18:46:16.67 SPR Module 1, FW Rev. B, FPGA Rev. C @18:46:17.36
Plunge.configure :default @18:46:17.48 SamplePump.configure :default @18:46:17.60 Toroid.configure :default @18:46:17.71 Carousel.configure :default @18:46:17.83 Abs.configure :default @18:46:17.95 PhaseSeparator.configure :default @18:46:18.10 Archive.configure :default @18:46:18.22 Air.configure :default @18:46:18.32 Lysis1.configure :default @18:46:18.41 Lysis2.configure :default @18:46:18.52 PinPort.configure :default @18:46:18.61 All elf controllers running firmware version 4.92 @18:46:18.67 Gate.power :motor,:ON,limit to 5000mA @18:46:19.06 Plunge.home.jog 0 @18:46:19.76 PinPort.home.jog 0 @18:46:19.80 Lysis1.home.jog 0 @18:46:19.84 Archive.home.jog 0 @18:46:19.91 Toroid.home.jog 0 @18:46:20.00 Lysis2.home.jog 0 @18:46:20.10 Carousel.home.jog 0 @18:46:20.17 Air.home.jog 0 @18:46:21.09 Plunge.in :homing @18:46:21.45 Plunge.jog 1596 @18:46:21.55 Lysis2.in :homing @18:46:21.61 Air.in :homing @18:46:21.68 Toroid.in :homing @18:46:21.75 PinPort.in :homing @18:46:21.81 Lysis1.in :homing @18:46:21.87 Archive.in :homing @18:46:22.19 Lysis2.jog 1404 @18:46:22.35 Air.jog 1376 @18:46:22.50 PinPort.jog -111 @18:46:22.57 Lysis1.jog 1400 @18:46:22.61 Archive.jog 1400 @18:46:22.67 Toroid.jog 630 @18:46:23.14 PinPort.setRawPosition! 0 @18:46:23.25 PinPort.seek :home @18:46:23.41 Plunge.setRawPosition! 50 @18:46:23.44 PinPort.in :default @18:46:23.59 Plunge.seek :home @18:46:23.76 Lysis2.setRawPosition! 50 @18:46:23.85 Lysis2.seek :home @18:46:23.98 Plunge.in :default @18:46:24.23 Lysis1.setRawPosition! 50 @18:46:24.27 Air.setRawPosition! 50 @18:46:24.32 Archive.setRawPosition! 50 @18:46:24.52 Lysis1.seek :home @18:46:24.58 Air.seek :home @18:46:25.12 Archive.seek :home @18:46:25.52 Lysis2.in :default @18:46:25.55 Lysis1.in :default @18:46:25.63 Toroid.setRawPosition! 0 @18:46:25.86 Toroid.seek :home @18:46:26.07 Archive.in :default @18:46:26.10 Toroid.in :default @18:46:26.15 Air.in :default @18:46:26.66 Toroid.seek :clear @18:46:28.40
Carousel.in :default @18:46:28.56 Carousel.home.seek 52 @18:46:28.87 Selecting Cartridge 51 @18:46:29.21 Carousel.seek 51 @18:46:29.79 Lysis1.in :fast @18:46:29.88 Lysis1.seek :locked @18:46:32.55 Plunge.seek :locked @18:46:32.77 Air.in :fast @18:46:32.87 Air.seek :locked @18:46:33.89
Carousel.reconfigure :locked @18:46:34.11 Powering cartridge bus @18:46:36.77 Gate.setIR 1750,5000,0,6 @18:46:36.91 Gate.setIR 0,5000,0,6 @18:46:37.01 PuckHeater.configure :default @18:46:37.12 Gate.power :heat,:ON,limit to 5000mA @18:46:37.74 Mounted lysis Cartridge @18:46:37.78 WARNING: Using default puck temperature sensor calibration @18:46:37.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:46:39.51 SPR.command "read_sequences" @18:46:40.19 SPR.command "stats_on" @18:46:40.39 SPR.command "start 0 1" @18:46:41.10 SPR.start /var/log/esp/spr24sep2318h.spr.bz2 @18:46:43.05 Waiting for baseline SPR stabilization @18:46:43.07 Handoff.select :waste @18:46:43.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:46:49.91 Syringe tare is 52% of full scale at 22C (drifted 1%) @18:46:49.97 PinPort.in :locking @18:46:50.10 PinPort.seek :locked @18:46:50.68 Archive.in :fast @18:46:50.81 Lysis2.in :fast @18:46:50.92 Lysis1.seek 1100uL @18:46:50.97 Archive.seek :full @18:46:51.19 Lysis2.seek 1100uL @18:46:55.54 delayUntil "18:56:42.61" @18:56:42.63 Lysis2.in :DAmix @18:56:42.79 Lysis1.in :DAmix @18:56:43.36 Lysis2.seek 950uL @18:56:43.42 Lysis1.seek 950uL @18:57:04.37 delay 5 seconds @18:57:09.39 Handoff.select SPR @18:57:09.57 SPR.command "start 2 14" @18:57:10.13 SPR stabilized @18:57:10.34 delay 10 seconds @18:57:20.36 SPR.command "start 2 0" @18:57:23.51 Waiting up to 11:00 for SPR to enter state 1 @18:59:21.17 SPR.command "hold_on" @18:59:22.56 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @18:59:23.23 Lysis2.seek 500uL @18:59:23.30 Lysis1.seek 500uL @19:00:25.59 delay 15 seconds @19:00:40.62 SPR.command "hold_off" @19:00:44.04 Waiting up to 1:00:00 for SPR to enter state 7 @19:01:25.64 Session Ended @19:16:59.58 SPRsummary:-1.1RIU,11.1RIU,31.5RIU,3.64RIU after 1920s @19:31:16.61 delay 10 seconds @19:31:26.64 SPR.command "start 2 0" @19:31:29.34 Waiting up to 11:00 for SPR to enter state 1 @19:33:27.01 SPR.command "hold_on" @19:33:28.39 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @19:33:29.11 Lysis2.seek 275uL @19:33:29.17 Lysis1.seek 275uL @19:34:00.39 delay 15 seconds @19:34:15.41 SPR.command "hold_off" @19:34:18.64 Waiting up to 1:00:00 for SPR to enter state 7 @20:04:51.25 delay 10 seconds @20:05:01.27 SPR.command "start 2 0" @20:05:03.97 Waiting up to 11:00 for SPR to enter state 1 @20:07:01.63 SPR.command "hold_on" @20:07:03.01 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @20:07:03.71 Lysis2.seek 50uL @20:07:03.80 Lysis1.seek 50uL @20:07:34.95 delay 15 seconds @20:07:49.97 SPR.command "hold_off" @20:07:53.26 Waiting up to 1:00:00 for SPR to enter state 7 @20:38:25.80 Additional SPR Analyses Skipped due to Insufficient Volume @20:38:26.08 Handoff.select :close @20:38:26.27
Gate.power :heat,:OFF @20:38:26.29 Gate.power :cartridge,:OFF @20:38:26.42 PinPort.in :unlocking @20:38:26.51 PinPort.seek :clear @20:38:27.01 Carousel.in :default @20:38:27.16 Carousel.home.seek 51 @20:38:27.52 Plunge.seek :locked @20:38:27.78 Archive.in :unlocking @20:38:27.93 Archive.seek :clear @20:38:28.10 Air.in :unlocking @20:38:28.27 Air.seek :clear @20:38:28.42 Lysis1.in :unlocking @20:38:28.59 Lysis1.seek :clear @20:38:28.76 Lysis2.in :unlocking @20:38:28.85 Lysis2.seek :clear @20:38:49.28 Ended log spr24sep2318h.spr.bz2 @20:38:49.30
SPR.command "stats_off" @20:38:49.94 Gate.power :analytic1,:OFF @20:38:49.96 Gate.power :motor,:OFF @20:38:50.02 Gate.power :core,:OFF @20:38:50.06 Gate.power :main,:OFF @20:38:50.09 Safely stopped and ready to power off @20:38:50.30 Gate.power :sampler,:OFF,limit to 3500mA @20:38:50.33 Gate.power :skimmer,:OFF,limit to 3500mA @20:38:53.87 Gate.power :main,:ON,limit to 5000mA @20:38:53.89 Gate.power :analytic1,:ON,limit to 4000mA @20:38:54.04 Powering core bus @20:38:56.35 SPR Module 1, FW Rev. B, FPGA Rev. C @20:38:56.82
Plunge.configure :default @20:38:56.92 SamplePump.configure :default @20:38:57.02 Toroid.configure :default @20:38:57.12 Carousel.configure :default @20:38:57.22 Abs.configure :default @20:38:57.33 PhaseSeparator.configure :default @20:38:57.43 Archive.configure :default @20:38:57.54 Air.configure :default @20:38:57.60 Lysis1.configure :default @20:38:57.67 Lysis2.configure :default @20:38:57.77 PinPort.configure :default @20:38:57.86 All elf controllers running firmware version 4.92 @20:38:57.88 Gate.power :motor,:ON,limit to 5000mA @20:38:58.24 Plunge.home.jog 0 @20:38:58.93 PinPort.home.jog 0 @20:38:58.97 Lysis1.home.jog 0 @20:38:59.04 Archive.home.jog 0 @20:38:59.08 Toroid.home.jog 0 @20:38:59.14 Lysis2.home.jog 0 @20:38:59.18 Carousel.home.jog 0 @20:38:59.24 Air.home.jog 0 @20:39:00.28 Plunge.in :homing @20:39:00.54 Plunge.jog 1601 @20:39:00.63 Lysis2.in :homing @20:39:00.67 Air.in :homing @20:39:00.73 Toroid.in :homing @20:39:00.77 PinPort.in :homing @20:39:00.81 Archive.in :homing @20:39:00.87 Lysis1.in :homing @20:39:01.19 Lysis2.jog 1407 @20:39:01.23 Air.jog 1382 @20:39:01.27 Toroid.jog 630 @20:39:01.33 PinPort.jog -109 @20:39:01.41 Archive.jog 1400 @20:39:01.47 Lysis1.jog 1399 @20:39:01.93 PinPort.setRawPosition! 0 @20:39:02.04 PinPort.seek :home @20:39:02.19 Plunge.setRawPosition! 50 @20:39:02.22 PinPort.in :default @20:39:02.37 Plunge.seek :home @20:39:02.68 Plunge.in :default @20:39:02.81 Lysis2.setRawPosition! 50 @20:39:02.85 Air.setRawPosition! 50 @20:39:03.01 Lysis2.seek :home @20:39:03.02 Air.seek :home @20:39:03.69 Lysis1.setRawPosition! 50 @20:39:03.82 Archive.setRawPosition! 50 @20:39:03.86 Lysis2.in :default @20:39:04.03 Lysis1.seek :home @20:39:04.17 Archive.seek :home @20:39:04.36 Toroid.setRawPosition! 0 @20:39:04.38 Air.in :default @20:39:04.53 Toroid.seek :home @20:39:04.70 Lysis1.in :default @20:39:04.75 Archive.in :default @20:39:04.81 Toroid.in :default @20:39:05.19 Toroid.seek :clear @20:39:06.94
Carousel.in :default @20:39:07.10 Carousel.home.seek 51 @20:39:07.38 Selecting Cartridge 50 @20:39:07.71 Carousel.seek 50 @20:39:08.27 Lysis1.in :fast @20:39:08.36 Lysis1.seek :locked @20:39:11.01 Plunge.seek :locked @20:39:11.20 Air.in :fast @20:39:11.28 Air.seek :locked @20:39:12.29
Carousel.reconfigure :locked @20:39:12.46 Powering cartridge bus @20:39:15.12 Gate.setIR 1750,5000,0,6 @20:39:15.25 Gate.setIR 0,5000,0,6 @20:39:15.34 PuckHeater.configure :default @20:39:15.43 Gate.power :heat,:ON,limit to 5000mA @20:39:16.08 Mounted lysis Cartridge @20:39:16.14 WARNING: Using default puck temperature sensor calibration @20:39:16.16 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:39:17.78 SPR.command "read_sequences" @20:39:18.01 SPR.command "stats_on" @20:39:18.20 SPR.command "start 0 1" @20:39:18.91 SPR.start /var/log/esp/spr24sep2320h.spr.bz2 @20:39:20.86 Waiting for baseline SPR stabilization @20:39:20.88 Handoff.select :waste @20:39:21.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:39:27.69 Syringe tare is 52% of full scale at 22C (drifted 1%) @20:39:27.73 PinPort.in :locking @20:39:27.83 PinPort.seek :locked @20:39:28.36 Archive.in :fast @20:39:28.47 Lysis2.in :fast @20:39:28.52 Lysis1.seek 1100uL @20:39:28.61 Archive.seek :full @20:39:28.77 Lysis2.seek 1100uL @20:39:33.14 delayUntil "20:49:20.41" @20:49:20.43 Lysis2.in :DAmix @20:49:20.57 Lysis1.in :DAmix @20:49:21.12 Lysis2.seek 950uL @20:49:21.19 Lysis1.seek 950uL @20:49:42.11 delay 5 seconds @20:49:47.14 Handoff.select SPR @20:49:47.30 SPR.command "start 2 14" @20:49:47.94 SPR stabilized @20:49:48.18 delay 10 seconds @20:49:58.20 SPR.command "start 2 0" @20:50:01.31 Waiting up to 11:00 for SPR to enter state 1 @20:51:58.98 SPR.command "hold_on" @20:52:00.36 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @20:52:01.03 Lysis2.seek 500uL @20:52:01.09 Lysis1.seek 500uL @20:53:03.37 delay 15 seconds @20:53:18.42 SPR.command "hold_off" @20:53:21.82 Waiting up to 1:00:00 for SPR to enter state 7 @21:09:37.39 SPRsummary:-1.57RIU,139RIU,283RIU,-2.38RIU after 1920s @21:23:54.44 delay 10 seconds @21:24:04.46 SPR.command "start 2 0" @21:24:07.14 Waiting up to 11:00 for SPR to enter state 1 @21:26:04.80 SPR.command "hold_on" @21:26:06.71 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @21:26:07.41 Lysis2.seek 275uL @21:26:07.46 Lysis1.seek 275uL @21:26:38.73 delay 15 seconds @21:26:53.75 SPR.command "hold_off" @21:26:56.44 Waiting up to 1:00:00 for SPR to enter state 7 @21:57:28.13 delay 10 seconds @21:57:38.15 SPR.command "start 2 0" @21:57:41.33 Waiting up to 11:00 for SPR to enter state 1 @21:59:39.43 SPR.command "hold_on" @21:59:40.81 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @21:59:41.48 Lysis2.seek 50uL @21:59:41.54 Lysis1.seek 50uL @22:00:12.77 delay 15 seconds @22:00:27.79 SPR.command "hold_off" @22:00:31.06 Waiting up to 1:00:00 for SPR to enter state 7 @22:31:03.60 Additional SPR Analyses Skipped due to Insufficient Volume @22:31:03.86 Handoff.select :close @22:31:04.05
Gate.power :heat,:OFF @22:31:04.07 Gate.power :cartridge,:OFF @22:31:04.20 PinPort.in :unlocking @22:31:04.28 PinPort.seek :clear @22:31:04.79 Carousel.in :default @22:31:04.94 Carousel.home.seek 50 @22:31:05.30 Plunge.seek :locked @22:31:05.56 Archive.in :unlocking @22:31:05.67 Archive.seek :clear @22:31:05.85 Air.in :unlocking @22:31:06.03 Air.seek :clear @22:31:06.18 Lysis1.in :unlocking @22:31:06.33 Lysis1.seek :clear @22:31:06.52 Lysis2.in :unlocking @22:31:06.63 Lysis2.seek :clear @22:31:27.50 Ended log spr24sep2320h.spr.bz2 @22:31:27.53
SPR.command "stats_off" @22:31:27.81 Gate.power :analytic1,:OFF @22:31:27.83 Gate.power :motor,:OFF @22:31:27.87 Gate.power :core,:OFF @22:31:27.91 Gate.power :main,:OFF @22:31:27.95 Safely stopped and ready to power off @22:31:28.26 Gate.power :sampler,:OFF,limit to 3500mA @22:31:28.29 Gate.power :skimmer,:OFF,limit to 3500mA @22:31:31.71 Gate.power :main,:ON,limit to 5000mA @22:31:31.74 Gate.power :analytic1,:ON,limit to 4000mA @22:31:31.84 Powering core bus @22:31:34.19 SPR Module 1, FW Rev. B, FPGA Rev. C @22:31:34.62
Plunge.configure :default @22:31:34.73 SamplePump.configure :default @22:31:35.39 Toroid.configure :default @22:31:35.49 Carousel.configure :default @22:31:35.59 Abs.configure :default @22:31:35.70 PhaseSeparator.configure :default @22:31:35.81 Archive.configure :default @22:31:35.91 Air.configure :default @22:31:35.98 Lysis1.configure :default @22:31:36.11 Lysis2.configure :default @22:31:36.22 PinPort.configure :default @22:31:36.30 All elf controllers running firmware version 4.92 @22:31:36.33 Gate.power :motor,:ON,limit to 5000mA @22:31:36.62 Plunge.home.jog 0 @22:31:37.40 PinPort.home.jog 0 @22:31:37.45 Lysis1.home.jog 0 @22:31:37.51 Archive.home.jog 0 @22:31:37.56 Toroid.home.jog 0 @22:31:37.62 Lysis2.home.jog 0 @22:31:37.66 Carousel.home.jog 0 @22:31:37.70 Air.home.jog 0 @22:31:38.54 Plunge.in :homing @22:31:38.89 Plunge.jog 1603 @22:31:39.11 Lysis2.in :homing @22:31:39.18 Lysis1.in :homing @22:31:39.23 Archive.in :homing @22:31:39.28 PinPort.in :homing @22:31:39.33 Toroid.in :homing @22:31:39.37 Air.in :homing @22:31:39.63 Lysis1.jog 1400 @22:31:39.70 Lysis2.jog 1406 @22:31:39.76 Archive.jog 1400 @22:31:39.81 PinPort.jog -111 @22:31:39.85 Toroid.jog 630 @22:31:39.90 Air.jog 1377 @22:31:40.40 Plunge.setRawPosition! 50 @22:31:40.48 Plunge.seek :home @22:31:40.60 PinPort.setRawPosition! 0 @22:31:40.68 PinPort.seek :home @22:31:40.85 Plunge.in :default @22:31:40.87 PinPort.in :default @22:31:41.17 Lysis1.setRawPosition! 50 @22:31:41.28 Lysis1.seek :home @22:31:41.43 Lysis2.setRawPosition! 50 @22:31:41.47 Air.setRawPosition! 50 @22:31:41.50 Archive.setRawPosition! 50 @22:31:41.71 Lysis2.seek :home @22:31:41.74 Air.seek :home @22:31:41.78 Archive.seek :home @22:31:42.15 Lysis1.in :default @22:31:42.18 Lysis2.in :default @22:31:42.41 Air.in :default @22:31:42.45 Archive.in :default @22:31:42.97 Toroid.setRawPosition! 0 @22:31:43.17 Toroid.seek :home @22:31:43.30 Toroid.in :default @22:31:43.46 Toroid.seek :clear @22:31:45.13
Carousel.in :default @22:31:45.28 Carousel.home.seek 50 @22:31:45.57 Selecting Cartridge 49 @22:31:45.89 Carousel.seek 49 @22:31:46.45 Lysis1.in :fast @22:31:46.52 Lysis1.seek :locked @22:31:49.17 Plunge.seek :locked @22:31:49.37 Air.in :fast @22:31:49.44 Air.seek :locked @22:31:50.45
Carousel.reconfigure :locked @22:31:50.62 Powering cartridge bus @22:31:53.28 Gate.setIR 1750,5000,0,6 @22:31:53.41 Gate.setIR 0,5000,0,6 @22:31:53.50 PuckHeater.configure :default @22:31:53.59 Gate.power :heat,:ON,limit to 5000mA @22:31:54.21 Mounted lysis Cartridge @22:31:54.27 WARNING: Using default puck temperature sensor calibration @22:31:54.29 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:31:55.90 SPR.command "read_sequences" @22:31:55.98 SPR.command "stats_on" @22:31:56.89 SPR.command "start 0 1" @22:31:57.60 SPR.start /var/log/esp/spr24sep2322h.spr.bz2 @22:31:59.64 Waiting for baseline SPR stabilization @22:31:59.66 Handoff.select :waste @22:31:59.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:32:06.48 Syringe tare is 52% of full scale at 22C (drifted 1%) @22:32:06.51 PinPort.in :locking @22:32:06.62 PinPort.seek :locked @22:32:07.15 Archive.in :fast @22:32:07.21 Lysis2.in :fast @22:32:07.30 Lysis1.seek 1100uL @22:32:07.44 Archive.seek :full @22:32:07.56 Lysis2.seek 1100uL @22:32:11.88 delayUntil "22:41:59.20" @22:41:59.22 Lysis2.in :DAmix @22:41:59.36 Lysis1.in :DAmix @22:41:59.90 Lysis2.seek 950uL @22:41:59.96 Lysis1.seek 950uL @22:42:20.91 delay 5 seconds @22:42:25.94 Handoff.select SPR @22:42:26.13 SPR.command "start 2 14" @22:42:26.72 SPR stabilized @22:42:26.91 delay 10 seconds @22:42:36.93 SPR.command "start 2 0" @22:42:40.09 Waiting up to 11:00 for SPR to enter state 1 @22:44:37.75 SPR.command "hold_on" @22:44:39.66 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @22:44:40.35 Lysis2.seek 500uL @22:44:40.40 Lysis1.seek 500uL @22:45:42.71 delay 15 seconds @22:45:57.73 SPR.command "hold_off" @22:46:00.61 Waiting up to 1:00:00 for SPR to enter state 7 @23:02:15.22 SPRsummary:-1.31RIU,115RIU,265RIU,1.25RIU after 1919s @23:16:32.26 delay 10 seconds @23:16:42.29 SPR.command "start 2 0" @23:16:44.98 Waiting up to 11:00 for SPR to enter state 1 @23:18:42.64 SPR.command "hold_on" @23:18:44.05 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @23:18:44.75 Lysis2.seek 275uL @23:18:44.79 Lysis1.seek 275uL @23:19:16.03 delay 15 seconds @23:19:31.06 SPR.command "hold_off" @23:19:34.28 Waiting up to 1:00:00 for SPR to enter state 7 @23:50:06.90 delay 10 seconds @23:50:16.92 SPR.command "start 2 0" @23:50:19.60 Waiting up to 11:00 for SPR to enter state 1 @23:52:17.26 SPR.command "hold_on" @23:52:18.65 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @23:52:19.36 Lysis2.seek 50uL @23:52:19.42 Lysis1.seek 50uL @23:52:50.64 delay 15 seconds @23:53:05.67 SPR.command "hold_off" @23:53:08.90 Waiting up to 1:00:00 for SPR to enter state 7 @00:23:41.46PDT24-Sep-24 Additional SPR Analyses Skipped due to Insufficient Volume @00:23:41.70 Handoff.select :close @00:23:41.89
Gate.power :heat,:OFF @00:23:41.91 Gate.power :cartridge,:OFF @00:23:42.07 PinPort.in :unlocking @00:23:42.15 PinPort.seek :clear @00:23:42.66 Carousel.in :default @00:23:42.80 Carousel.home.seek 49 @00:23:43.18 Plunge.seek :locked @00:23:43.45 Archive.in :unlocking @00:23:43.60 Archive.seek :clear @00:23:43.73 Air.in :unlocking @00:23:43.86 Air.seek :clear @00:23:44.05 Lysis1.in :unlocking @00:23:44.20 Lysis1.seek :clear @00:23:44.36 Lysis2.in :unlocking @00:23:44.45 Lysis2.seek :clear @00:24:05.19 Ended log spr24sep2322h.spr.bz2 @00:24:05.22
SPR.command "stats_off" @00:24:05.63 Gate.power :analytic1,:OFF @00:24:05.65 Gate.power :motor,:OFF @00:24:05.69 Gate.power :core,:OFF @00:24:05.72 Gate.power :main,:OFF @00:24:05.75 Safely stopped and ready to power off @00:24:05.94 Cartridge::None in MAIN -- Not enough standard cartridges remain @00:24:06.08 Session Ended @12:00:01.85PDT18-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @12:00:03.57 >BREAK< ESP3 Gateway v1.44 for core bus @12:00:03.60 >BREAK< ESP3 Gateway v1.44 for cartridge bus @12:00:07.21 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @12:00:07.89 Sea pressure (Abs) tare is 6% of full scale @12:00:12.57 Polling Can every 2:00 @12:00:12.63 Waiting for espclients... Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @13:35:24.66PDT18-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:35:26.39 >BREAK< ESP3 Gateway v1.44 for core bus @13:35:26.43 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:35:30.22 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:35:30.89 Sea pressure (Abs) tare is 6% of full scale @13:35:48.15PDT18-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @13:35:49.78 >BREAK< ESP3 Gateway v1.44 for core bus @13:35:49.81 >BREAK< ESP3 Gateway v1.44 for cartridge bus @13:35:53.27 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @13:35:53.95 Sea pressure (Abs) tare is 6% of full scale @13:35:58.55 Polling Can every 2:00 @13:35:59.08 Clients may connect on TCP port 9999 @13:35:59.10 Waiting for espclients... @13:36:25.01 Session Begun @13:37:02.08 -> exit @13:37:02.11 Session Ended @13:41:22.99 Session Begun @13:45:33.31 Session Begun @13:51:40.25 Session Begun @13:51:49.99 -> showlog @13:52:39.14 -> who :who @13:52:42.77 -> whom :whom @13:53:01.06 -> #what? @13:56:09.40 -> exit @13:56:09.43 Session Ended @14:11:47.69 -> MainThread.exit # @14:11:47.86 ThreadError in preston -- killed thread @14:11:48.01 Session Ended @14:11:48.05 Session Ended @14:14:52.94PDT18-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:14:54.64 >BREAK< ESP3 Gateway v1.44 for core bus @14:14:54.66 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:14:58.22 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:14:58.90 Sea pressure (Abs) tare is 6% of full scale @14:15:03.55 Polling Can every 2:00 @14:15:03.59 Waiting for espclients... Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @23:59:02.68PDT18-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @23:59:06.46 >BREAK< ESP3 Gateway v1.44 for core bus @23:59:06.49 >BREAK< ESP3 Gateway v1.44 for cartridge bus @23:59:10.26 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @23:59:11.03 Sea pressure (Abs) tare is 6% of full scale @23:59:17.44 Polling Can every 2:00 @23:59:18.04 Clients may connect on TCP port 9999 @23:59:18.06 Waiting for espclients... @23:59:58.70 Session Begun @00:01:13.32PDT19-Jul-26 -> exit @00:01:13.34 Session Ended @00:01:20.85 Session Begun @00:01:26.53 -> showlog 50 @00:01:41.47 -> MainThread.exit # @00:01:41.58 ThreadError in brent -- killed thread @00:01:41.65 Session Ended @09:15:36.74PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @09:15:38.43 >BREAK< ESP3 Gateway v1.44 for core bus @09:15:38.46 >BREAK< ESP3 Gateway v1.44 for cartridge bus @09:15:41.94 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @09:15:42.67 Sea pressure (Abs) tare is 6% of full scale @09:15:47.48 Polling Can every 2:00 @09:15:47.52 Waiting for espclients... @09:16:43.48 Session Begun @09:16:46.62 -> slots {[36,4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [28,26,24,22,20] => { Type: :aRNAl_bac, State: :reserved, was: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :reserved, was: :dry}, 48 => { Type: :microSPR_phyto, State: :dry}, 50 => { Type: :microSPR_phyto, State: :leaky}, 52 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr26jul1900h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @09:17:17.51 -> start {fire lysis, archive, archive} # @09:17:17.90 Gate.power :analytic1,:ON,limit to 4000mA @09:17:17.92 Gate.power :analytic2,:ON,limit to 4000mA @09:17:17.95 Gate.power :analytic3,:ON,limit to 4000mA @09:17:18.57 SPR Module 1, FW Rev. B, FPGA Rev. C @09:17:18.79 Plunge.configure :default @09:17:18.88 Plunge.hold @09:17:18.98 Plunge.configure :default @09:17:19.16 SamplePump.configure :default @09:17:19.34 Toroid.configure :default @09:17:19.43 Toroid.hold @09:17:19.53 Toroid.configure :default @09:17:19.72 Carousel.configure :default @09:17:19.81 Carousel.hold @09:17:19.92 Carousel.configure :default @09:17:20.10 Abs.configure :default @09:17:20.28 PhaseSeparator.configure :default @09:17:20.49 Archive.configure :default @09:17:20.58 Archive.hold @09:17:20.70 Archive.configure :default @09:17:21.29 Air.configure :default @09:17:21.37 Air.hold @09:17:21.46 Air.configure :default @09:17:21.60 Lysis1.configure :default @09:17:21.68 Lysis1.hold @09:17:21.80 Lysis1.configure :default @09:17:21.95 Lysis2.configure :default @09:17:22.03 Lysis2.hold @09:17:22.14 Lysis2.configure :default @09:17:22.36 PinPort.configure :default @09:17:22.48 PinPort.hold @09:17:22.60 PinPort.configure :default @09:17:22.69 All elf controllers running firmware version 4.94 @09:17:23.47 Toroid.seek :clear @09:17:23.68 Air.seek :clear @09:17:28.44 Lysis1.seek :clear @09:17:32.50 Lysis2.seek :clear @09:17:32.63 Archive.seek :clear @09:17:32.75 Carousel.in :default @09:17:32.91 Carousel.home.seek 50 @09:17:33.29 Selecting Cartridge 48 @09:17:33.42 Gate.power :heat,:OFF @09:17:33.47 Gate.power :cartridge,:OFF @09:17:33.59 Carousel.in :default @09:17:33.74 Carousel.home.seek 50 @09:17:34.43 Plunge.in :unlocking @09:17:34.53 Plunge.seek :clear @09:17:35.54 Carousel.seek 48 @09:17:36.33 Carousel.reconfigure :align @09:17:36.53 Lysis1.in :fast @09:17:36.62 Lysis1.seek :locked @09:17:39.28 Plunge.in :default @09:17:39.43 Plunge.seek :locked @09:17:39.58 Air.in :fast @09:17:39.67 Air.seek :locked @09:17:40.77 Carousel.reconfigure :locked @09:17:40.95 Powering cartridge bus @09:17:44.61 Gate.setIR 1750,5000,0,6 @09:17:44.74 Gate.setIR 0,5000,0,6 @09:17:44.84 PuckHeater.configure :default @09:17:44.94 Gate.power :heat,:ON,limit to 5000mA @09:17:45.68 Mounted lysis Cartridge board#J59949 @09:17:45.74 Using default cartridge heat loss curve for HeatingFunctionInCarousel @09:17:46.10 Preparing to filter microSPR_phyto cartridge in slot 48 @09:17:46.42 Air.in :default @09:17:46.57 Air.seek :sealed @09:17:46.70 Priming sample loop w/200ml, bypass w/10ml @09:17:46.72 SamplePump.setPosition! 0ml @09:17:46.81 Exhaust.close @09:17:46.94 Bypass.open @09:17:49.28 Intake.open @09:17:50.96 Sea 22.3m deep (47.6psia) @09:17:50.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:17:53.63 Vacuum tare is 64% of full scale at 24C (drifted 12%) @09:17:53.82 Intake.close @09:17:53.87 Bypass.close @09:17:55.60 Verifying toroid holds at least 0.6..0.85 of 47.6psia @09:17:55.63 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @09:18:00.23 Toroid pressure fell 4% to 45.5psia @09:18:00.25 Bypass.open @09:18:01.83 Intake.open @09:18:04.20 Abs.in :default @09:18:04.29 Abs.hold @09:18:04.60 SamplePump.in :start @09:18:04.70 SamplePump.seek 10ml,1:10 @09:18:05.29 SamplePump.configure :prime @09:18:17.28 Abs.stop @09:18:17.35 SamplePump.setPosition! 0ml @09:18:17.43 Exhaust.open @09:18:19.64 Bypass.close @09:18:22.05 Abs.in :default @09:18:22.13 Abs.hold @09:18:22.32 Sea 22.3m deep (47.7psia) @09:18:22.58 SamplePump.in :start @09:18:22.69 SamplePump.seek 200ml,13:50 @09:18:23.28 SamplePump.configure :prime @09:22:26.01 Abs.stop @09:22:26.13 Bypass.open @09:22:27.69 Intake.close @09:22:29.42 Toroid.in :hold @09:22:29.50 Toroid.seek :crack @09:22:32.01 Toroid.seek :cartridge @09:22:34.16 Toroid.in :default @09:22:34.25 Toroid.seek :clear @09:22:39.02 Exhaust.close @09:22:39.25 Bypass.close @09:22:41.21 Verifying cartridge holds at least 0.65..0.85 of 47.4psia @09:22:41.30 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @09:22:46.01 Sampling 1000.0ml @09:22:46.03 SamplePump.setPosition! 0ml @09:22:46.14 Exhaust.open @09:22:47.66 Intake.open @09:22:49.46 Abs.in :default @09:22:49.54 Abs.hold @09:22:49.70 Sea 22.2m deep (47.5psia) @09:22:49.93 SamplePump.in :start @09:22:50.03 SamplePump.seek 1000ml,56:04 @09:22:50.62 SamplePump.configure :sample @09:22:53.03 Cartridge 48 filtering 2/1000ml at 0.74ml/s with 12.5psi vacuum 20.9m deep (45.6psia) @09:23:53.08 Cartridge 48 filtering 42/1000ml at 0.67ml/s with 12.5psi vacuum 23.9m deep (50.0psia) @09:24:53.08 Cartridge 48 filtering 82/1000ml at 0.66ml/s with 11.1psi vacuum 25.0m deep (51.7psia) @09:25:53.08 Cartridge 48 filtering 121/1000ml at 0.65ml/s with 8.8psi vacuum 25.2m deep (52.0psia) @09:26:53.07 Cartridge 48 filtering 160/1000ml at 0.65ml/s with 4.9psi vacuum 21.8m deep (46.9psia) @09:27:53.08 Cartridge 48 filtering 198/1000ml at 0.64ml/s with 8.9psi vacuum 23.6m deep (49.5psia) @09:28:53.08 Cartridge 48 filtering 236/1000ml at 0.64ml/s with 9.6psi vacuum 21.1m deep (45.8psia) @09:29:53.08 Cartridge 48 filtering 274/1000ml at 0.63ml/s with 10.6psi vacuum 26.8m deep (54.3psia) @09:30:53.07 Cartridge 48 filtering 312/1000ml at 0.63ml/s with 1.4psi vacuum 25.0m deep (51.6psia) @09:31:53.08 Cartridge 48 filtering 349/1000ml at 0.62ml/s with 13.3psi vacuum 20.3m deep (44.7psia) @09:32:53.07 Cartridge 48 filtering 387/1000ml at 0.62ml/s with 2.2psi vacuum 23.6m deep (49.6psia) @09:33:53.08 Cartridge 48 filtering 423/1000ml at 0.62ml/s with 4.2psi vacuum 26.1m deep (53.3psia) @09:34:53.51 Cartridge 48 filtering 461/1000ml at 0.62ml/s with 10.1psi vacuum 22.2m deep (47.5psia) @09:35:53.07 Cartridge 48 filtering 497/1000ml at 0.61ml/s with 12.6psi vacuum 23.2m deep (49.0psia) @09:36:53.08 Cartridge 48 filtering 533/1000ml at 0.61ml/s with 0.6psi vacuum 24.9m deep (51.5psia) @09:37:53.08 Cartridge 48 filtering 570/1000ml at 0.61ml/s with 3.8psi vacuum 25.2m deep (51.9psia) @09:38:53.08 Cartridge 48 filtering 606/1000ml at 0.61ml/s with 9.4psi vacuum 22.0m deep (47.2psia) @09:39:53.08 Cartridge 48 filtering 642/1000ml at 0.60ml/s with 12.7psi vacuum 21.1m deep (45.9psia) @09:40:53.07 Cartridge 48 filtering 678/1000ml at 0.60ml/s with 8.4psi vacuum 24.1m deep (50.3psia) @09:41:53.08 Cartridge 48 filtering 715/1000ml at 0.60ml/s with 15.6psi vacuum 17.0m deep (39.8psia) @09:42:53.17 Cartridge 48 filtering 751/1000ml at 0.60ml/s with 13.4psi vacuum 17.3m deep (40.3psia) @09:43:53.08 Cartridge 48 filtering 787/1000ml at 0.60ml/s with 15.6psi vacuum 20.5m deep (45.0psia) @09:44:53.08 Cartridge 48 filtering 822/1000ml at 0.60ml/s with 13.7psi vacuum 26.0m deep (53.1psia) @09:45:53.09 Cartridge 48 filtering 858/1000ml at 0.60ml/s with 15.4psi vacuum 26.4m deep (53.7psia) @09:46:53.08 Cartridge 48 filtering 894/1000ml at 0.60ml/s with 10.3psi vacuum 24.1m deep (50.2psia) @09:47:53.07 Cartridge 48 filtering 930/1000ml at 0.60ml/s with 10.0psi vacuum 17.8m deep (41.0psia) @09:48:53.07 Cartridge 48 filtering 965/1000ml at 0.59ml/s with 3.9psi vacuum 22.5m deep (48.0psia) @09:49:52.14 Abs.stop @09:49:52.26 Intake.close @09:49:52.31 Exhaust.close @09:49:52.66 Toroid.in :hold @09:49:52.76 Toroid.seek :bypass @09:49:56.38 Toroid.in :default @09:49:56.50 Toroid.seek :clear @09:50:00.10 Sampled 1000.0ml @09:50:01.79 SPR.command "read_sequences" @09:50:02.10 SPR.command "stats_on" @09:50:02.27 SPR.command "start 0 1" @09:50:02.98 SPR.start /var/log/esp/spr26jul1909h.spr.bz2 @09:50:04.95 Handoff.select :waste @09:50:05.13 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:50:12.46 Syringe tare is 51% of full scale at 24C (drifted 0%) @09:50:12.50 PinPort.in :locking @09:50:12.60 PinPort.seek :locked @09:50:13.47 Archive.in :fast @09:50:13.54 Lysis2.in :fast @09:50:13.64 Lysis1.seek 1250uL @09:50:13.79 Archive.seek :empty @09:50:13.88 Lysis2.seek 950uL @09:50:22.49 Evacuating puck with filter bubble point of 20psi @09:50:22.56 PinPort.seek :locked @09:50:22.68 Handoff.select :waste @09:50:22.72 Plunge.seek :puckBottom @09:50:24.07 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:50:30.67 Air syringe pumping up to 1 strokes to reach 4psi @09:50:30.69 Air stroke #1 of 1 @09:50:30.82 Air.in :pressurize @09:50:30.90 Air.seek :pressurized @09:50:36.19 0.5psi after pressurize @09:50:36.21 Air.in :compress @09:50:36.31 Air.seek :evac @09:50:42.98 0.9psi after compress @09:50:55.08 0.1psi after compress (stable) @09:50:55.10 Air.in :default @09:50:55.19 Air.hold @09:50:55.42 at -0.2psi Opened to waste @09:50:55.44 Handoff.select :blocked @09:50:55.62 Plunge.seek :puckTop @09:50:56.55 Air syringe pumping up to 8 strokes to reach 20psi @09:50:56.58 Air stroke #1 of 8 @09:50:56.72 Air.in :compress @09:50:56.81 Air.seek :evac @09:50:56.99 1.2psi after compress @09:51:07.59 1.7psi after compress (stable) @09:51:07.62 Air.in :gulp @09:51:07.72 Air.seek :topVent @09:51:14.22 1.8psi after gulp @09:51:19.82 2.0psi after gulp (stable) @09:51:19.84 Air stroke #2 of 8 @09:51:19.98 Air.in :pressurize @09:51:20.06 Air.seek :pressurized @09:51:26.49 1.8psi after pressurize @09:51:26.52 Air.in :compress @09:51:26.62 Air.seek :evac @09:51:33.26 12.8psi after compress @09:51:46.37 15.9psi after compress (stable) @09:51:46.43 Air.in :gulp @09:51:46.53 Air.seek :topVent @09:51:53.04 16.2psi after gulp @09:51:59.14 16.2psi after gulp (stable) @09:51:59.15 Air stroke #3 of 8 @09:51:59.29 Air.in :pressurize @09:51:59.39 Air.seek :pressurized @09:52:05.79 16.1psi after pressurize @09:52:05.82 Air.in :compress @09:52:05.92 Air.seek :evac @09:52:11.10 Reached 20.4 of 20.0psi after pumping 5240uL (2.95 strokes) @09:52:11.12 Air.in :default @09:52:11.23 Air.hold @09:52:11.39 Plunge.seek :puckBottom @09:52:12.29 delay 3 seconds @09:52:15.31 Handoff.select :waste @09:52:15.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:52:33.09 delay 1:00 @09:53:33.11 Handoff.select :blocked @09:53:33.28 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @09:53:55.86 Handoff.select :waste @09:53:56.08 0.1psi at evacEnd @09:53:56.12 Plunge.seek :pTop @09:53:57.05 delay 5 seconds @09:54:02.08 Air.in :glide @09:54:02.16 Air.seek :fullVent @09:54:04.76 delay 2 seconds @09:54:06.81 Plunge.seek :pBottom @09:54:07.70 Seawater evacuated, Pressure above/below filter released. @09:54:07.73 Plunge.seek :locked @09:54:09.08 Handoff.select :waste @09:54:09.21 Lysis1.in :slow @09:54:09.30 Lysis1.seek 950uL @09:54:23.39 delay 5 seconds @09:54:28.44 Handoff.select :blocked @09:54:28.61 Plunge.seek :puckTop @09:54:30.14 Lysis1.seek 350uL @09:54:58.18 delay 3 seconds @09:55:01.27 Air.in mix with 2.79934 parts Lysis1 @09:55:01.96 Air.seek :evac @09:55:02.01 Lysis1.seek 190uL @09:55:09.76 Air.in :fast @09:55:09.85 Air.seek :full @09:55:16.43 Lysis1.seek :empty @09:55:25.58 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @17.0psi @09:55:25.60 Plunge.seek :puckBottom @09:55:26.65 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @15.3psi @09:55:26.67 Polling Can every 2:00 @09:55:27.24 PuckHeater.configure :default #ambient 23.8C @09:55:27.34 PuckHeater.seek 81.6C @09:56:58.42 PuckHeater.hold 2:00,81.6C @09:58:58.03 Polling Can every 2:00 @09:58:58.58 PuckHeater.configure :default #ambient 23.9C @09:58:58.67 PuckHeater.seek 80.5C @09:58:58.86 PuckHeater.hold 2:00,80.5C @10:00:58.47 Polling Can every 2:00 @10:00:59.05 PuckHeater.hold 8:00,79.5C @10:08:57.19 Lysate cooked @13.6psi @10:08:57.21 Plunge.seek :puckTop @10:08:58.16 Air syringe pumping up to 5 strokes to reach 40psi @10:08:58.17 Air stroke #1 of 5 @10:08:58.33 Air.in :pressurize @10:08:58.45 Air.seek :pressurized @10:09:04.87 13.2psi after pressurize @10:09:05.34 Air.in :compress @10:09:05.47 Air.seek :evac @10:09:11.33 Reached 41.2 of 40.0psi after pumping 1728uL (0.97 strokes) @10:09:11.35 Air.in :default @10:09:11.46 Air.hold @10:09:11.58 delay 2 seconds @10:09:13.61 Air.in :fast @10:09:13.70 Air.seek :full @10:09:20.10 Plunge.seek :puckBottom @10:09:21.07 Lysis1.seek 138uL @10:09:27.65 delay 2 seconds @10:09:29.68 Plunge.seek :puckTop @10:09:30.59 Lysis1.seek :empty @10:09:37.19 Plunge.seek :puckBottom @10:09:38.35 Lysis1.in :extract @10:09:38.54 Lysis1.seek 675uL,nil @10:09:42.75 Air.in :pressurize @10:09:42.85 Air.seek :pressurized @10:09:43.28 Syringe::LysatePlungerStuck in PROCESSING -- at < 482uL during Lysis Syringe #1 (LyS1) move @10:09:43.32 Lysis1.in :default @10:09:43.43 Lysis1.seek 452uL @10:09:49.31 12.2psi after pressurize @10:09:49.34 Air.in :compressLysate @10:09:49.44 Air.seek :evac @10:10:02.08 Reached 15.7 of 15.5psi after pumping 1583uL (0.89 strokes) @10:10:02.10 Air.in :default @10:10:02.20 Air.hold @10:10:02.40 Lysis1.in :extract @10:10:02.56 Lysis1.seek 675uL,nil @10:10:04.58 Air.in :compressLysate @10:10:04.70 Air.seek :evac @10:10:05.10 Syringe::LysatePlungerStuck in PROCESSING -- at < 614uL during Lysis Syringe #1 (LyS1) move @10:10:05.16 Lysis1.in :default @10:10:05.25 Lysis1.seek 584uL @10:10:06.71 Reached 14.6 of 15.5psi after pumping 1608uL (0.91 strokes) @10:10:06.75 Air.in :default @10:10:06.85 Air.hold @10:10:07.16 Air.in :compressLysate @10:10:07.25 Air.seek :evac @10:10:08.29 Reached 15.6 of 15.5psi after pumping 1620uL (0.91 strokes) @10:10:08.31 Air.in :default @10:10:08.45 Air.hold @10:10:08.66 Lysis1.in :extract @10:10:08.77 Lysis1.seek 675uL,nil @10:10:10.85 Pulled 675uL lysate @13.7psi @10:10:11.05 Lysis1.seek 800uL,nil @10:10:11.35 Air.in :compressLysate @10:10:11.44 Air.seek :evac @10:10:18.63 Air.in :default @10:10:18.71 Air.hold @10:10:19.05 Air.in :glide @10:10:19.13 Air.seek :fullVent @10:10:21.41 Handoff.select :waste @10:10:21.57 Recovered 800uL lysate @14.7psi @10:10:21.60 delay 3 seconds @10:10:24.74 After 3 delay Recovered 800uL lysate @0.5psi @10:10:24.77 Plunge.seek :locked @10:10:26.12 Lysis2.in :DAmix @10:10:26.27 Lysis1.in :DAmix @10:10:27.04 Lysis2.seek 700uL @10:10:27.10 Lysis1.seek 550uL @10:11:02.23 delay 5 seconds @10:11:07.25 Handoff.select SPR @10:11:07.42 SPR.command "start 2 14" @10:11:09.15 SPR stabilized @10:11:09.35 delay 20 seconds @10:11:29.38 SPR.command "start 2 0" @10:11:32.92 Waiting up to 11:00 for SPR to enter state 1 @10:13:31.02 SPR.command "hold_on" @10:13:32.94 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @10:13:33.65 Lysis2.seek 400uL @10:13:33.69 Lysis1.seek 250uL @10:14:15.71 delay 15 seconds @10:14:30.74 SPR.command "hold_off" @10:14:34.01 Waiting up to 1:00:00 for SPR to enter state 7 @10:29:09.09 Session Begun @10:29:11.39 -> slots {[36,4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [28,26,24,22,20] => { Type: :aRNAl_bac, State: :reserved, was: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :reserved, was: :dry}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000.0, lysed: 800, spr: spr26jul1909h.spr.bz2}, 50 => { Type: :microSPR_phyto, State: :leaky}, 52 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr26jul1900h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @10:29:45.40 -> Cart.dry [34,32] @10:29:52.80 -> Cart.reserve Cart.dry 2 @10:29:54.88 -> slots {[36,4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [28,26,24,22,20] => { Type: :aRNAl_bac, State: :reserved, was: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :reserved, was: :dry}, 48 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000.0, lysed: 800, spr: spr26jul1909h.spr.bz2}, 50 => { Type: :microSPR_phyto, State: :leaky}, 52 => { Type: :microSPR_phyto, State: :lysing, filtered: 1000, spr: "spr26jul1900h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @10:30:05.36 -> exit @10:30:05.38 Session Ended @10:30:48.41 Session Begun @10:30:48.66 SPRsummary:-29RIU,-13.9RIU,-21.2RIU,-18.8RIU,high,high,high after 3265s @10:35:45.45 -> BACsampler.prime 10, 30 @10:35:45.62 Priming sample loop w/10ml, bypass w/30ml @10:35:45.66 SamplePump.setPosition! 0ml @10:35:45.79 Bypass.open @10:35:48.23 Intake.open @10:35:50.60 Cartridge::Sampler::Warning in preston -- Delta Pressure @-31.0psi @10:35:51.86 Cartridge::Sampler::Leaky in preston -- Delta Pressure @-31.4psi indicates Sensor Failure or serious Leak @10:36:18.12 -> Intake.open Intake is opened @10:36:20.51 -> Exhaust.open @10:36:20.54 Exhaust.open Exhaust is opened @10:36:23.13 -> PB.open Bypass (PB) is opened @10:36:33.62 -> Sampler.tare! @10:36:33.76 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:36:36.43 Vacuum tare is 54% of full scale at 23C (drifted 2%) 0.004987691(PSI/count)-176.4 @10:36:42.77 -> BACsampler.prime 10, 30 @10:36:42.90 Priming sample loop w/10ml, bypass w/30ml @10:36:42.92 SamplePump.setPosition! 0ml @10:36:43.04 Exhaust.close @10:36:45.49 Abs.in :default @10:36:45.59 Abs.hold @10:36:45.88 SamplePump.in :start @10:36:46.01 SamplePump.seek 30ml,2:30 @10:36:46.57 SamplePump.configure :prime @10:37:22.90 Abs.stop @10:37:23.03 SamplePump.setPosition! 0ml @10:37:23.16 Exhaust.open @10:37:25.42 Bypass.close @10:37:27.92 Abs.in :default @10:37:28.03 Abs.hold @10:37:28.44 SamplePump.in :start @10:37:28.55 SamplePump.seek 10ml,1:10 @10:37:29.12 SamplePump.configure :prime @10:37:41.16 Abs.stop BACsampler @10:38:21.58 -> BACsampler.prime 400, nil @10:38:21.76 Priming sample loop w/400ml @10:38:21.80 SamplePump.setPosition! 0ml @10:38:21.94 Exhaust.close @10:38:23.48 Bypass.open @10:38:25.98 Exhaust.open @10:38:28.21 Bypass.close @10:38:30.65 Abs.in :default @10:38:30.75 Abs.hold @10:38:31.74 SamplePump.in :start @10:38:31.88 SamplePump.seek 400ml,27:10 @10:38:32.43 SamplePump.configure :prime @10:45:05.60 SPR analysis completed @10:45:11.26 Ended log spr26jul1909h.spr.bz2 @10:45:11.31 SPR.command "stats_off" @10:45:11.79 Cartridge::None in detached from bill -- No dry archiveHiBiomass_bac cartridges remain @10:45:11.82 Waiting to Resume from Checkpoint "Selecting Cartridge 48" @10:46:38.02 Abs.stop BACsampler @10:48:36.17 Session Begun @10:48:39.00 -> SP Sample Pump (SP) at 400ml @10:49:43.35 -> SP.status @10:50:44.99 -> BACsampler.prime 400, nil @10:50:45.15 Priming sample loop w/400ml @10:50:45.17 SamplePump.setPosition! 0ml @10:50:45.35 Exhaust.close @10:50:46.90 Bypass.open @10:50:49.38 Exhaust.open @10:50:51.69 Bypass.close @10:50:54.14 Abs.in :default @10:50:54.26 Abs.hold @10:50:54.65 SamplePump.in :start @10:50:54.77 SamplePump.seek 400ml,27:10 @10:50:55.34 SamplePump.configure :prime @10:55:18.67 Session Begun @10:55:22.76 -> Handoff Handoff Valve::Manifold selects Analytical Module @10:55:30.32 -> Handoff.waste @10:55:30.35 Handoff.select :waste Handoff Valve::Manifold selects waste @10:55:41.31 -> Handoff.AM @10:55:41.34 Handoff.select :AM Handoff Valve::Manifold selects Analytical Module @10:55:43.85 -> Handoff.waste @10:55:43.88 Handoff.select :waste Handoff Valve::Manifold selects waste @10:55:48.46 -> Handoff.AM @10:55:48.49 Handoff.select :AM Handoff Valve::Manifold selects Analytical Module @10:55:51.93 -> Handoff.waste @10:55:52.01 Handoff.select :waste Handoff Valve::Manifold selects waste @10:55:54.88 -> Handoff.AM @10:55:54.96 Handoff.select :AM Handoff Valve::Manifold selects Analytical Module @10:56:03.27 -> Handoff.blocked @10:56:03.31 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:56:08.70 -> Handoff.waste @10:56:08.77 Handoff.select :waste Handoff Valve::Manifold selects waste @10:56:11.69 -> Handoff.blocked @10:56:11.71 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:56:14.05 -> Handoff.waste @10:56:14.11 Handoff.select :waste Handoff Valve::Manifold selects waste @10:56:17.54 -> Handoff.blocked @10:56:17.57 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:56:19.47 -> Handoff.waste @10:56:19.50 Handoff.select :waste Handoff Valve::Manifold selects waste @10:56:21.08 -> Handoff.blocked @10:56:21.17 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:56:24.55 -> Handoff.waste @10:56:24.60 Handoff.select :waste Handoff Valve::Manifold selects waste @10:56:26.24 -> Handoff.blocked @10:56:26.27 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:56:28.26 -> Handoff.waste @10:56:28.29 Handoff.select :waste Handoff Valve::Manifold selects waste @10:59:00.99 Abs.stop BACsampler @11:05:36.55 -> SP.status.position 519467 @11:07:47.61 -> MainThread.exit @11:07:47.83 Intake.close @11:07:47.90 Exhaust.close @11:07:47.99 Handoff.select :close # @11:07:49.51 -> esp :esp @11:07:49.85 Session Ended @11:07:49.95 Session Ended @11:07:50.07 Session Ended @11:07:50.13 Session Ended @14:07:40.06PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:07:43.82 >BREAK< ESP3 Gateway v1.44 for core bus @14:07:43.84 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:07:47.57 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:07:48.30 Sea pressure (Abs) tare is 6% of full scale @14:07:53.56 Polling Can every 2:00 @14:07:53.62 Waiting for espclients... @14:07:54.65 Session Begun @14:08:02.04 -> ESP.ready! @14:08:02.11 Gate.power :sampler,:OFF,limit to 3500mA @14:08:02.13 Gate.power :main,:ON,limit to 5000mA @14:08:02.17 Gate.power :analytic1,:ON,limit to 4000mA @14:08:02.21 Gate.power :analytic2,:ON,limit to 4000mA @14:08:02.44 Powering core bus @14:08:04.69 SPR Module 1, FW Rev. B, FPGA Rev. C @14:08:05.23 Plunge.configure :default @14:08:05.34 SamplePump.configure :default @14:08:05.45 Toroid.configure :default @14:08:05.56 Carousel.configure :default @14:08:05.67 Abs.configure :default @14:08:05.77 PhaseSeparator.configure :default @14:08:05.89 Archive.configure :default @14:08:06.00 Air.configure :default @14:08:06.08 Lysis1.configure :default @14:08:06.17 Lysis2.configure :default @14:08:06.30 PinPort.configure :default @14:08:06.40 All elf controllers running firmware version 4.94 @14:08:06.44 Gate.power :motor,:ON,limit to 5000mA @14:08:06.76 Plunge.home.jog 0 @14:08:07.99 Lysis2.home.jog 0 @14:08:08.05 Carousel.home.jog 0 @14:08:08.10 PinPort.home.jog 0 @14:08:08.13 Lysis1.home.jog 0 @14:08:08.17 Air.home.jog 0 @14:08:08.22 Toroid.home.jog 0 @14:08:08.29 Archive.home.jog 0 @14:08:09.10 Plunge.in :homing @14:08:09.47 Plunge.jog 588 @14:08:09.56 PinPort.in :homing @14:08:09.61 Lysis2.in :homing @14:08:09.64 Air.in :homing @14:08:09.70 Toroid.in :homing @14:08:09.74 Lysis1.in :homing @14:08:09.79 Archive.in :homing @14:08:10.12 PinPort.jog 1225 @14:08:10.18 Lysis2.jog 1621 @14:08:10.23 Air.jog 1427 @14:08:10.29 Lysis1.jog 1599 @14:08:10.36 Toroid.jog 1370 @14:08:10.47 Archive.jog 305 @14:08:10.87 Plunge.setRawPosition! 50 @14:08:10.95 Plunge.seek :home @14:08:11.25 Plunge.in :default @14:08:11.36 Archive.setRawPosition! 50 @14:08:11.43 Archive.seek :home @14:08:11.73 Archive.in :default @14:08:11.86 Air.setRawPosition! 0 @14:08:11.95 Air.seek :home @14:08:12.11 Lysis2.setRawPosition! 50 @14:08:12.14 Lysis1.setRawPosition! 50 @14:08:12.19 Air.in :default @14:08:12.40 Lysis2.seek :home @14:08:12.45 Lysis1.seek :home @14:08:12.69 PinPort.setRawPosition! 3150 @14:08:12.77 PinPort.seek :home @14:08:12.94 Lysis1.in :default @14:08:12.97 Lysis2.in :default @14:08:15.21 Toroid.setRawPosition! 0 @14:08:15.28 Toroid.seek :home @14:08:15.40 Toroid.in :default @14:08:17.19 PinPort.in :default @14:08:17.55 Toroid.seek :clear @14:08:17.72 PinPort.in :unlocking @14:08:17.80 PinPort.seek :clear @14:08:21.18 Air.seek :clear @14:08:21.30 Lysis1.seek :clear @14:08:22.05 Lysis2.seek :clear @14:08:22.86 Archive.seek :clear @14:08:23.67 Carousel.in :default @14:08:23.82 Carousel.home.seek 22 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 22 Abs = Absolute Pressure (Leak) Sensor @17psia PhaseSeparator = Intake Head Pressure @2.31psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:08:25.20 -> Power..sampler :of @14:08:25.25 SyntaxError in preston -- compile error preston:2: syntax error, unexpected tSYMBEG, expecting kDO or '{' or '(' Power..sampler :of ^ @14:08:27.36 -> Power..sampler :off @14:08:27.38 SyntaxError in preston -- compile error preston:3: syntax error, unexpected tSYMBEG, expecting kDO or '{' or '(' Power..sampler :off ^ @14:08:32.92 -> Power.sampler :off @14:08:32.96 Gate.power :sampler,:OFF false @14:08:36.78 -> Power.sampler :on @14:08:36.81 Gate.power :sampler,:ON true @14:08:41.97 -> Power.sampler :off @14:08:41.99 Gate.power :sampler,:OFF false @14:08:54.72 -> Power.sampler :on @14:08:54.75 Gate.power :sampler,:ON true @14:09:37.97 -> Power.sampler :off @14:09:38.00 Gate.power :sampler,:OFF false @14:10:27.33 -> Power.sampler :on @14:10:27.37 Gate.power :sampler,:ON true @14:11:44.98 -> Sampler.refill @14:11:45.00 Bypass.open @14:11:46.52 Intake.open @14:11:48.11 SamplePump.setPosition! 0ml @14:11:48.27 SamplePump.seek 30ml @14:12:28.31 Bypass.close @14:12:29.85 Exhaust.open @14:12:31.47 SamplePump.seek 55ml @14:12:50.50 Session Begun @14:12:56.85 -> Cart.reserve 2,4 2 @14:13:01.01 -> Cart.reserved [4,2] @14:13:04.88 delay 1 second @14:13:05.91 Intake.close @14:13:06.00 Exhaust.close Sample Pump (SP) at 55ml @14:13:16.42 -> BACsampler.prime @14:13:16.57 Priming sample loop w/200ml, bypass w/10ml @14:13:16.59 SamplePump.setPosition! 0ml @14:13:16.73 Bypass.open @14:13:19.09 Intake.open @14:13:20.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:13:23.29 Vacuum tare is 54% of full scale at 24C (drifted 2%) @14:13:23.50 Abs.in :default @14:13:23.60 Abs.hold @14:13:23.72 PhaseSeparator.in :full @14:13:23.83 PhaseSeparator.hold @14:13:24.11 SamplePump.in :start @14:13:24.23 SamplePump.seek 10ml,1:10 @14:13:24.81 SamplePump.configure :prime @14:13:37.95 Abs.stop @14:13:38.05 PhaseSeparator.stop @14:13:38.14 SamplePump.setPosition! 0ml @14:13:38.24 Exhaust.open @14:13:40.48 Bypass.close @14:13:42.91 Abs.in :default @14:13:43.01 Abs.hold @14:13:43.14 PhaseSeparator.hold @14:13:43.52 SamplePump.in :start @14:13:43.62 SamplePump.seek 200ml,13:50 @14:13:44.20 SamplePump.configure :prime @14:15:10.87 SamplePump.hold @14:15:11.20 Intake.close @14:15:11.26 Exhaust.close @14:15:12.98 Abs.stop @14:15:13.08 PhaseSeparator.stop @14:15:13.20 UserAbort in preston -- Sample Pump (SP) seek to 200ml Aborted! @14:15:17.90 -> Power.sampler :off @14:15:18.44 Gate.power :sampler,:OFF false @14:15:35.68 -> PhytoSampler.goalVolume =100 100 @14:15:57.66 -> Cart.dry Cart.type=:lyseHold_bac [22] @14:16:13.40 -> slots {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 22 => { Type: :lyseHold_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @14:16:26.32 -> Cart.unlock @14:16:26.47 Carousel.in :default @14:16:26.63 Carousel.home.seek 22 @14:16:27.30 Plunge.in :unlocking @14:16:27.42 Plunge.seek :clear @14:16:33.25 -> SC Storage Carousel (SC) at 22 @14:16:46.86 -> slots 22=>:aRNAl_bac {22 => { Type: :aRNAl_bac, State: :dry}} @14:16:48.32 -> slots {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @14:17:05.70 -> lysis :microSPR_phyto @14:17:25.78 -> Cart.dry Cart.type=lyseHold_phyto [42,40,22] @14:17:29.91 -> slot {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [42,40,22] => { Type: :lyseHold_phyto, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @14:17:39.54 -> slot 22=>nil {22 => nil} @14:17:53.70 -> slot 22=>[:aRNAl_bac, :dry] {22 => { Type: :aRNAl_bac, State: :dry}} @14:17:55.65 -> slots {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @14:18:27.06 -> start {fire :lyseHold_phyto} # @14:18:28.12 Toroid.seek :clear @14:18:28.39 Air.seek :clear @14:18:28.54 Lysis1.seek :clear @14:18:28.68 Lysis2.seek :clear @14:18:28.82 Archive.seek :clear @14:18:28.95 Carousel.in :default @14:18:29.12 Carousel.home.seek 22 @14:18:29.45 Selecting Cartridge 42 @14:18:29.80 Carousel.seek 42 @14:18:34.32 Carousel.reconfigure :align @14:18:34.49 Lysis1.in :fast @14:18:34.60 Lysis1.seek :locked @14:18:37.27 Plunge.in :default @14:18:37.43 Plunge.seek :locked @14:18:37.59 Air.in :fast @14:18:37.69 Air.seek :locked @14:18:38.79 Carousel.reconfigure :locked @14:18:39.01 Powering cartridge bus @14:18:41.67 Gate.setIR 1750,5000,0,6 @14:18:41.81 Gate.setIR 0,5000,0,6 @14:18:41.92 PuckHeater.configure :default @14:18:42.49 Gate.power :heat,:ON,limit to 5000mA @14:18:42.77 Mounted lysis Cartridge board#J60008 @14:18:42.84 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:18:43.31 Preparing to filter lyseHold_phyto cartridge in slot 42 @14:18:43.61 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:21:40.91 PressureError in FILTERING -- Pressure Failed to Stabilize @14:21:40.96 Waiting to Resume from Checkpoint "Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi" @14:23:42.83 -> Power {analytic1: true, analytic2: true, cartridge: true, core: true, heat: true, main: true, motor: true, sampler: false} @14:24:25.68 Session Begun @14:24:30.77 -> Sampler.source {PSI: /home/esp/esp2/utils/cartridge/sampler.rb:278, addTweak: /home/esp/esp2/utils/cartridge/sampler.rb:52, adjustMaxPSIa: /home/esp/esp2/utils/post3g.rb:146, age: /home/esp/esp2/utils/cartridge/sampler.rb:154, applyTweaks: /home/esp/esp2/utils/cartridge/sampler.rb:118, avgRate: /home/esp/esp2/utils/cartridge/sampler.rb:57, avgRate=: /home/esp/esp2/utils/cartridge/sampler.rb:57, awaitHead: /home/esp/esp2/utils/cartridge/late.rb:66, baseLeakCheck!: /home/esp/esp2/utils/cartridge/sampler.rb:322, baseTare!: /home/esp/esp2/utils/cartridge/sampler.rb:293, baseTare?: /home/esp/esp2/utils/cartridge/sampler.rb:285, bubblePt: /home/esp/esp2/utils/cartridge/sampler.rb:57, bubblePt=: /home/esp/esp2/utils/cartridge/sampler.rb:57, bypassVac: /home/esp/esp2/utils/cartridge/sampler.rb:57, bypassVac=: /home/esp/esp2/utils/cartridge/sampler.rb:57, bypassVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, bypassVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, calibrate: /home/esp/esp2/utils/cartridge/deep.rb:96, cancelSPRstop: /home/esp/esp2/protocol/3G/function/spr.rb:71, charge: /home/esp/esp2/utils/cartridge/deep.rb:203, chargeBleed: /home/esp/esp2/utils/cartridge/sampler.rb:57, chargeBleed=: /home/esp/esp2/utils/cartridge/sampler.rb:57, chargeDelay: /home/esp/esp2/utils/cartridge/sampler.rb:57, chargeDelay=: /home/esp/esp2/utils/cartridge/sampler.rb:57, chargeVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, chargeVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, checkCartridge: /home/esp/esp2/utils/cartridge/deep.rb:136, checkMinPress: /home/esp/esp2/utils/cartridge/deep.rb:170, checkToroid: /home/esp/esp2/utils/cartridge/deep.rb:147, clog: /home/esp/esp2/utils/cartridge/sampler.rb:220, closeValves: /home/esp/esp2/utils/cartridge/sampler.rb:358, config: /home/esp/esp2/utils/cartridge/sampler.rb:100, config=: /home/esp/esp2/utils/cartridge/sampler.rb:100, daRecoverMixedLysate: /home/esp/esp2/protocol/3G/function/spr.rb:263, daSPR: /home/esp/esp2/protocol/3G/function/spr.rb:43, defaultTarget: /home/esp/esp2/utils/cartridge/sampler.rb:382, deliverLysis: /home/esp/esp2/protocol/3G/function/LyseGo.rb:150, deltaPress: /home/esp/esp2/utils/cartridge/sampler.rb:369, depthCheck: /home/esp/esp2/utils/cartridge/sampler.rb:106, doSPR: /home/esp/esp2/protocol/3G/function/spr.rb:197, evac2waste: /home/esp/esp2/protocol/3G/function/LyseGo.rb:50, evacPSIrate: /home/esp/esp2/utils/cartridge/sampler.rb:57, evacPSIrate=: /home/esp/esp2/utils/cartridge/sampler.rb:57, evacPSIrise: /home/esp/esp2/utils/cartridge/sampler.rb:57, evacPSIrise=: /home/esp/esp2/utils/cartridge/sampler.rb:57, evacSWpumpTo: /home/esp/esp2/protocol/3G/function/LyseGo.rb:81, filter: /home/esp/esp2/utils/cartridge/sampler.rb:399, finalLysingPressure: /home/esp/esp2/utils/cartridge/sampler.rb:57, finalLysingPressure=: /home/esp/esp2/utils/cartridge/sampler.rb:57, flowDelay: /home/esp/esp2/utils/cartridge/sampler.rb:57, flowDelay=: /home/esp/esp2/utils/cartridge/sampler.rb:57, goalVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, goalVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, initialLysatePull: /home/esp/esp2/utils/cartridge/sampler.rb:57, initialLysatePull=: /home/esp/esp2/utils/cartridge/sampler.rb:57, initialize: /home/esp/esp2/utils/cartridge/sampler.rb:377, lastTarePSIa: /home/esp/esp2/utils/cartridge/deep.rb:89, leakCheck: /home/esp/esp2/utils/cartridge/deep.rb:157, leakFlood: /home/esp/esp2/utils/cartridge/sampler.rb:57, leakFlood=: /home/esp/esp2/utils/cartridge/sampler.rb:57, logInterval: /home/esp/esp2/utils/cartridge/sampler.rb:57, logInterval=: /home/esp/esp2/utils/cartridge/sampler.rb:57, logSeaPressure: /home/esp/esp2/utils/cartridge/deep.rb:71, logSingle: /home/esp/esp2/utils/cartridge/deep.rb:79, logText: /home/esp/esp2/utils/cartridge/carousel.rb:199, logTextBase: /home/esp/esp2/utils/cartridge/sampler.rb:225, lyse: /home/esp/esp2/protocol/3G/function/LyseGo.rb:367, lyseDA: /home/esp/esp2/protocol/3G/function/LyseGo.rb:406, lyseHold: /home/esp/esp2/protocol/3G/function/LyseGo.rb:396, lyseHoldBS: /home/esp/esp2/protocol/3G/function/LyseGo.rb:402, lyseOnly: /home/esp/esp2/protocol/3G/function/LyseGo.rb:341, lysisNull: /home/esp/esp2/protocol/3G/function/LyseGo.rb:37, maxAge: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxAge=: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxDuration: /home/esp/esp2/utils/cartridge/sampler.rb:450, maxEvacTries: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxEvacTries=: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxFlow: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxFlow=: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxPSIa: /home/esp/esp2/utils/post3g.rb:159, maxPostEvacPSI: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxPostEvacPSI=: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxPrimeDuration: /home/esp/esp2/utils/cartridge/sampler.rb:442, maxRecoveryTime: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxRecoveryTime=: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxSampleDuration: /home/esp/esp2/utils/cartridge/sampler.rb:446, maxSecsPerPSI: /home/esp/esp2/utils/cartridge/sampler.rb:57, maxSecsPerPSI=: /home/esp/esp2/utils/cartridge/sampler.rb:57, microSPR: /home/esp/esp2/protocol/3G/function/spr.rb:38, minDuration: /home/esp/esp2/utils/cartridge/sampler.rb:57, minDuration=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minOvershootDelay: /home/esp/esp2/utils/cartridge/sampler.rb:57, minOvershootDelay=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minPSIa: /home/esp/esp2/utils/cartridge/sampler.rb:57, minPSIa=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minPrimeRate: /home/esp/esp2/utils/cartridge/sampler.rb:57, minPrimeRate=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minRate: /home/esp/esp2/utils/cartridge/sampler.rb:57, minRate=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minRateDuration: /home/esp/esp2/utils/cartridge/sampler.rb:57, minRateDuration=: /home/esp/esp2/utils/cartridge/sampler.rb:57, minVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, minVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, needTare?: /home/esp/esp2/utils/cartridge/deep.rb:114, needsPrime: /home/esp/esp2/utils/cartridge/sampler.rb:146, openFlow: /home/esp/esp2/utils/cartridge/sampler.rb:627, prepareLGcart: /home/esp/esp2/protocol/3G/function/LyseGo.rb:20, pressureCheck: /home/esp/esp2/utils/cartridge/sampler.rb:457, pressureCheck!: /home/esp/esp2/utils/cartridge/sampler.rb:461, prime: /home/esp/esp2/utils/cartridge/deep.rb:14, primeVac: /home/esp/esp2/utils/cartridge/sampler.rb:57, primeVac=: /home/esp/esp2/utils/cartridge/sampler.rb:57, primeVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, primeVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, psi: /home/esp/esp2/utils/cartridge/sampler.rb:278, pump: /home/esp/esp2/utils/cartridge/sampler.rb:365, pump!: /home/esp/esp2/utils/cartridge/sampler.rb:482, pumpSeek: /home/esp/esp2/utils/cartridge/sampler.rb:623, pumpedVolume: /home/esp/esp2/utils/cartridge/sampler.rb:140, rangeChk: /home/esp/esp2/utils/cartridge/sampler.rb:172, recordPressureExtrema: /home/esp/esp2/utils/cartridge/sampler.rb:331, recoverLysate: /home/esp/esp2/protocol/3G/function/LyseGo.rb:296, recoverMoreLysate: /home/esp/esp2/protocol/3G/function/LyseGo.rb:183, recoveryBubblePt: /home/esp/esp2/utils/cartridge/sampler.rb:57, recoveryBubblePt=: /home/esp/esp2/utils/cartridge/sampler.rb:57, recoveryPressure: /home/esp/esp2/utils/cartridge/sampler.rb:57, recoveryPressure=: /home/esp/esp2/utils/cartridge/sampler.rb:57, recoveryVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, recoveryVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, reduceRate: /home/esp/esp2/utils/cartridge/sampler.rb:57, reduceRate=: /home/esp/esp2/utils/cartridge/sampler.rb:57, refill: /home/esp/esp2/utils/cartridge/deep.rb:120, reprime: /home/esp/esp2/utils/cartridge/sampler.rb:150, reprimeVolume: /home/esp/esp2/utils/cartridge/sampler.rb:57, reprimeVolume=: /home/esp/esp2/utils/cartridge/sampler.rb:57, retries: /home/esp/esp2/utils/cartridge/sampler.rb:57, retries=: /home/esp/esp2/utils/cartridge/sampler.rb:57, retry: /home/esp/esp2/utils/cartridge/sampler.rb:736, retryPinEngage!: /home/esp/esp2/protocol/3G/function/LyseGo.rb:330, retrySyringeEngage!: /home/esp/esp2/protocol/3G/function/LyseGo.rb:336, runSPR: /home/esp/esp2/protocol/3G/function/spr.rb:216, sample!: /home/esp/esp2/utils/cartridge/sampler.rb:643, sampleAge: /home/esp/esp2/utils/cartridge/sampler.rb:154, sampleQuietly: /home/esp/esp2/utils/cartridge/sampler.rb:387, sensorCheck: /home/esp/esp2/utils/cartridge/sampler.rb:373, servo: /home/esp/esp2/utils/cartridge/sampler.rb:572, skipSPRlysis: /home/esp/esp2/protocol/3G/function/spr.rb:48, stabilize: /home/esp/esp2/utils/cartridge/sampler.rb:160, startLogging: /home/esp/esp2/utils/cartridge/sampler.rb:233, stopLogging: /home/esp/esp2/utils/cartridge/sampler.rb:266, stopSPR: /home/esp/esp2/protocol/3G/function/spr.rb:60, stopSrcPump: /home/esp/esp2/utils/cartridge/late.rb:46, tare: /home/esp/esp2/utils/cartridge/deep.rb:96, tare!: /home/esp/esp2/utils/cartridge/sampler.rb:304, tareHead: /home/esp/esp2/utils/cartridge/late.rb:53, tarePSIa: /home/esp/esp2/utils/cartridge/deep.rb:89, targetVac: /home/esp/esp2/utils/cartridge/sampler.rb:114, targetVac=: /home/esp/esp2/utils/cartridge/sampler.rb:110, totalVolume: /home/esp/esp2/utils/cartridge/sampler.rb:143, tweak: /home/esp/esp2/utils/cartridge/sampler.rb:47, updateMinDeltaPress: /home/esp/esp2/utils/cartridge/sampler.rb:179, vacuum: /home/esp/esp2/utils/cartridge/sampler.rb:57, vacuum=: /home/esp/esp2/utils/cartridge/sampler.rb:57, wasteCount: /home/esp/esp2/utils/cartridge/sampler.rb:57, wasteCount=: /home/esp/esp2/utils/cartridge/sampler.rb:57, watchHead: /home/esp/esp2/utils/cartridge/late.rb:59, watchPSIa: /home/esp/esp2/utils/cartridge/deep.rb:238} @14:24:58.59 -> sampler.source @14:24:58.67 NoMethodError in preston#3 -- undefined method `source' for :sampler:Symbol @14:25:12.55 -> Cart.sampler PhytoSampler @14:25:19.40 -> exit @14:25:19.43 Session Ended @14:27:04.30 -> exit @14:27:04.34 Session Ended @14:28:58.59 -> MainThread.exit # @14:28:58.80 ThreadError in preston -- killed thread @14:28:58.87 Session Ended @14:29:09.07PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:29:10.80 >BREAK< ESP3 Gateway v1.44 for core bus @14:29:10.83 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:29:14.38 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:29:15.07 Sea pressure (Abs) tare is 6% of full scale @14:29:20.17 Polling Can every 2:00 @14:29:20.22 Waiting for espclients... @14:29:21.28 Session Begun @14:29:26.54 -> slot {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @14:29:43.90 -> start {fire :lyseHold_phyto} # @14:29:44.31 Gate.power :sampler,:OFF,limit to 3500mA @14:29:44.34 Gate.power :skimmer,:OFF,limit to 3500mA @14:29:44.38 Gate.power :analytic1,:ON,limit to 4000mA @14:29:45.14 Plunge.configure :default @14:29:45.23 SPR Module 1, FW Rev. B, FPGA Rev. C @14:29:45.29 Plunge.hold @14:29:45.39 Plunge.configure :default @14:29:45.56 SamplePump.configure :default @14:29:45.73 Toroid.configure :default @14:29:45.82 Toroid.hold @14:29:45.92 Toroid.configure :default @14:29:46.09 Carousel.configure :default @14:29:46.18 Carousel.hold @14:29:46.33 Carousel.configure :default @14:29:46.50 Abs.configure :default @14:29:47.09 PhaseSeparator.configure :default @14:29:47.30 Archive.configure :default @14:29:47.39 Archive.hold @14:29:47.49 Archive.configure :default @14:29:47.66 Air.configure :default @14:29:47.75 Air.hold @14:29:47.85 Air.configure :default @14:29:47.99 Lysis1.configure :default @14:29:48.07 Lysis1.hold @14:29:48.17 Lysis1.configure :default @14:29:48.35 Lysis2.configure :default @14:29:48.44 Lysis2.hold @14:29:48.54 Lysis2.configure :default @14:29:48.71 PinPort.configure :default @14:29:48.80 PinPort.hold @14:29:48.91 PinPort.configure :default @14:29:49.00 All elf controllers running firmware version 4.94 @14:29:49.74 Toroid.seek :clear @14:29:49.95 Air.seek :clear @14:29:52.44 Lysis1.seek :clear @14:29:56.51 Lysis2.seek :clear @14:29:56.64 Archive.seek :clear @14:29:56.76 Carousel.in :default @14:29:56.90 Carousel.home.seek 42 @14:29:57.29 Selecting Cartridge 42 @14:29:57.42 Gate.power :heat,:OFF @14:29:57.44 Gate.power :cartridge,:OFF @14:29:57.57 Carousel.in :default @14:29:57.71 Carousel.home.seek 42 @14:29:58.39 Plunge.in :unlocking @14:29:58.49 Plunge.seek :clear @14:29:59.53 Carousel.seek 42 @14:29:59.84 Carousel.reconfigure :align @14:30:00.00 Lysis1.in :fast @14:30:00.09 Lysis1.seek :locked @14:30:02.75 Plunge.in :default @14:30:02.91 Plunge.seek :locked @14:30:03.02 Air.in :fast @14:30:03.10 Air.seek :locked @14:30:04.21 Carousel.reconfigure :locked @14:30:04.43 Powering cartridge bus @14:30:08.29 Gate.setIR 1750,5000,0,6 @14:30:08.44 Gate.setIR 0,5000,0,6 @14:30:08.54 PuckHeater.configure :default @14:30:08.63 Gate.power :heat,:ON,limit to 5000mA @14:30:08.89 Mounted lysis Cartridge board#J60008 @14:30:08.96 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:30:09.30 Preparing to filter lyseHold_phyto cartridge in slot 42 @14:30:09.57 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:30:16.19 Intake Head Pressure tare is 0% of full scale at 25C (drifted -1%) @14:30:16.21 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:30:16.31 PhaseSeparator.in :filling @14:30:16.39 PhaseSeparator.hold @14:30:16.49 Gate.power :sampler,:ON @14:30:25.02 Head pressure reached 1.73psi in 9 seconds @14:30:25.06 Air.in :default @14:30:25.24 Air.seek :sealed @14:30:25.28 Priming sample loop w/200ml, bypass w/10ml @14:30:25.35 SamplePump.setPosition! 0ml @14:30:25.48 Exhaust.close @14:30:25.59 Bypass.open @14:30:27.88 -> Head.psi @14:30:27.92 Intake.open 1.7329436760933 @14:30:29.67 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:30:30.95 -> Head.psi 1.74471386042456 @14:30:31.86 -> Head.psi 1.75108487763139 @14:30:32.35 Vacuum tare is 54% of full scale at 25C (drifted 2%) @14:30:32.54 Abs.in :default @14:30:32.61 Abs.hold @14:30:32.66 -> Head.psi @14:30:32.85 PhaseSeparator.in :full 1.74017856004004 @14:30:32.99 PhaseSeparator.hold @14:30:33.24 Waiting for sampler to finish priming... @14:30:33.25 delayUntil "14:31:16.51" @14:30:33.96 -> Head.psi 1.7471974773018 @14:30:35.09 -> Head.psi 1.75065294426144 @14:30:36.01 -> Head.psi 1.75950757834551 @14:30:36.89 -> Head.psi 1.7016285067716 @14:30:37.78 -> Head.psi 1.73359157614823 @14:30:38.58 -> Head.psi 1.7471974773018 @14:30:39.45 -> Head.psi 1.73510334294307 @14:30:40.25 -> Head.psi 1.70270834019648 @14:30:42.15 -> Head.psi 1.69352975608495 @14:30:43.11 -> Head.psi 1.69601337296219 @14:30:44.05 -> Head.psi 1.70972725745824 @14:30:45.04 -> Head.psi 1.7149104578977 @14:30:46.04 -> Head.psi 1.72581677548905 @14:30:52.76 -> Head.status @14:31:05.90 -> Head.counts 15759 @14:31:15.78 -> Head.psi 1.5989363480649 @14:31:16.61 SamplePump.in :start @14:31:16.71 SamplePump.seek 10ml,1:10 @14:31:17.30 SamplePump.configure :prime @14:31:27.68 -> Head.psi 2.79819934974372 @14:31:29.22 -> Head.psi 2.84430823698637 @14:31:30.29 Abs.stop @14:31:30.40 -> Head.psi @14:31:30.52 PhaseSeparator.stop @14:31:30.61 SamplePump.setPosition! 0ml @14:31:30.69 Exhaust.open 2.75263037921352 @14:31:32.90 Bypass.close @14:31:33.37 -> Head.psi 2.6054490834015 @14:31:34.54 -> Head.psi 2.44185431953122 @14:31:35.29 Abs.in :default @14:31:35.37 Abs.hold @14:31:35.48 PhaseSeparator.hold @14:31:35.86 SamplePump.in :start @14:31:35.89 -> Head.psi @14:31:36.02 SamplePump.seek 200ml,13:50 2.48839514014382 @14:31:36.53 SamplePump.configure :prime @14:31:40.53 -> Head.counts 22703 @14:31:53.36 SamplePump.stop @14:31:53.53 Cartridge::Sampler::LostHeadPressure in FILTERING -- Head Pressure Fell to 0.86psi @14:31:53.56 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:31:53.63 PhaseSeparator.in :filling @14:31:53.72 PhaseSeparator.hold @14:31:53.83 Gate.power :sampler,:OFF @14:31:54.22 Gate.power :sampler,:ON @14:32:07.25 -> Head.counts 10690 @14:32:09.25 Gate.power :sampler,:OFF @14:32:09.64 Gate.power :sampler,:ON @14:32:09.72 -> Head.counts 1246 @14:32:14.65 -> Head.counts 10745 @14:32:24.67 Gate.power :sampler,:OFF @14:32:25.06 Gate.power :sampler,:ON @14:32:30.06 Head pressure reached 2.70psi in 37 seconds @14:32:30.08 Abs.hold @14:32:30.21 PhaseSeparator.in :full @14:32:30.33 PhaseSeparator.hold @14:32:30.69 SamplePump.in :start @14:32:30.79 SamplePump.seek 200ml,12:59 @14:32:31.38 SamplePump.configure :prime @14:32:52.91 -> MainThread.exit @14:32:52.95
SamplePump.stop # @14:32:53.28 SamplePump.stop @14:32:53.34 Exhaust.close @14:32:53.41 Intake.close @14:32:53.45 SamplePump.stop @14:32:55.03 Exhaust.close @14:32:55.24 Intake.close @14:32:55.31 ThreadError in closeIntake -- deferring killed thread @14:32:55.34 until Intake finishes closing @14:32:55.39 Session Ended @14:32:55.60 Abs.stop @14:33:09.72PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:33:11.49 >BREAK< ESP3 Gateway v1.44 for core bus @14:33:11.52 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:33:15.10 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:33:15.81 Sea pressure (Abs) tare is 6% of full scale @14:33:21.09 Polling Can every 2:00 @14:33:21.15 Waiting for espclients... @14:33:22.93 Session Begun @14:33:38.49 -> Head.counts 4377 @14:33:41.72 -> Head.counts 4660 @14:33:43.67 -> Head.counts 4516 @14:34:02.98 -> Power.sampler :off @14:34:03.00 Gate.power :sampler,:OFF false @14:34:33.41 -> Head.counts 122 @14:39:53.37 -> MainThread.exit # @14:39:53.47 ThreadError in preston -- killed thread @14:39:53.55 Session Ended @14:40:07.27PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:40:09.10 >BREAK< ESP3 Gateway v1.44 for core bus @14:40:09.13 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:40:12.81 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:40:13.49 Sea pressure (Abs) tare is 6% of full scale @14:40:18.77 Polling Can every 2:00 @14:40:18.82 Waiting for espclients... @14:40:20.31 Session Begun @14:40:36.01 -> Power.sampler :on @14:40:36.03 Gate.power :sampler,:ON true @14:41:23.22 -> Power.sampler :off @14:41:23.24 Gate.power :sampler,:OFF false @14:41:28.06 -> start {fire :lyseHold_phyto} # @14:41:28.49 Gate.power :sampler,:OFF,limit to 3500mA @14:41:28.51 Gate.power :skimmer,:OFF,limit to 3500mA @14:41:28.54 Gate.power :analytic1,:ON,limit to 4000mA @14:41:29.29 Plunge.configure :default @14:41:29.39 Plunge.hold @14:41:29.49 Plunge.configure :default @14:41:29.68 SamplePump.configure :default @14:41:29.89 SPR Module 1, FW Rev. B, FPGA Rev. C @14:41:29.94 Toroid.configure :default @14:41:30.04 Toroid.hold @14:41:30.13 Toroid.configure :default @14:41:30.33 Carousel.configure :default @14:41:30.42 Carousel.hold @14:41:30.52 Carousel.configure :default @14:41:30.69 Abs.configure :default @14:41:30.86 PhaseSeparator.configure :default @14:41:31.49 Archive.configure :default @14:41:31.58 Archive.hold @14:41:31.68 Archive.configure :default @14:41:31.85 Air.configure :default @14:41:31.93 Air.hold @14:41:32.03 Air.configure :default @14:41:32.17 Lysis1.configure :default @14:41:32.28 Lysis1.hold @14:41:32.40 Lysis1.configure :default @14:41:32.54 Lysis2.configure :default @14:41:32.63 Lysis2.hold @14:41:32.74 Lysis2.configure :default @14:41:32.91 PinPort.configure :default @14:41:32.99 PinPort.hold @14:41:33.09 PinPort.configure :default @14:41:33.18 All elf controllers running firmware version 4.94 @14:41:33.95 Toroid.seek :clear @14:41:34.14 Air.seek :clear @14:41:38.89 Lysis1.seek :clear @14:41:42.95 Lysis2.seek :clear @14:41:43.08 Archive.seek :clear @14:41:43.19 Carousel.in :default @14:41:43.34 Carousel.home.seek 42 @14:41:43.70 Selecting Cartridge 42 @14:41:43.84 Gate.power :heat,:OFF @14:41:43.86 Gate.power :cartridge,:OFF @14:41:44.00 Carousel.in :default @14:41:44.14 Carousel.home.seek 42 @14:41:44.79 Plunge.in :unlocking @14:41:44.88 Plunge.seek :clear @14:41:45.89 Carousel.seek 42 @14:41:46.20 Carousel.reconfigure :align @14:41:46.39 Lysis1.in :fast @14:41:46.48 Lysis1.seek :locked @14:41:49.16 Plunge.in :default @14:41:49.25 Plunge.seek :locked @14:41:49.46 Air.in :fast @14:41:49.54 Air.seek :locked @14:41:50.63 Carousel.reconfigure :locked @14:41:50.81 Powering cartridge bus @14:41:54.94 Gate.setIR 1750,5000,0,6 @14:41:55.07 Gate.setIR 0,5000,0,6 @14:41:55.16 PuckHeater.configure :default @14:41:55.26 Gate.power :heat,:ON,limit to 5000mA @14:41:55.52 Mounted lysis Cartridge board#J60008 @14:41:55.58 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:41:55.92 Preparing to filter lyseHold_phyto cartridge in slot 42 @14:41:56.20 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:42:02.82 Intake Head Pressure tare is 0% of full scale at 25C (drifted 1%) @14:42:02.84 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:42:02.91 PhaseSeparator.in :filling @14:42:02.98 PhaseSeparator.hold @14:42:03.09 Gate.power :sampler,:ON @14:42:11.69 Head pressure reached 2.18psi in 9 seconds @14:42:11.73 Air.in :default @14:42:11.91 Air.seek :sealed @14:42:11.94 Priming sample loop w/200ml, bypass w/10ml @14:42:12.04 SamplePump.setPosition! 0ml @14:42:12.14 Exhaust.close @14:42:12.28 Bypass.open @14:42:14.60 Intake.open @14:42:16.25 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:42:18.92 Vacuum tare is 54% of full scale at 25C (drifted 2%) @14:42:19.11 Abs.in :default @14:42:19.19 Abs.hold @14:42:19.29 PhaseSeparator.in :full @14:42:19.37 PhaseSeparator.hold @14:42:19.63 Waiting for sampler to finish priming... @14:42:19.64 delayUntil "14:43:03.11" @14:43:03.18 SamplePump.in :start @14:43:03.29 SamplePump.seek 10ml,1:10 @14:43:03.87 SamplePump.configure :prime @14:43:16.85 Abs.stop @14:43:16.93 PhaseSeparator.stop @14:43:17.02 SamplePump.setPosition! 0ml @14:43:17.09 Exhaust.open @14:43:19.31 Bypass.close @14:43:22.08 Abs.in :default @14:43:22.17 Abs.hold @14:43:22.31 PhaseSeparator.hold @14:43:22.76 SamplePump.in :start @14:43:22.87 SamplePump.seek 200ml,13:50 @14:43:23.46 SamplePump.configure :prime @14:43:50.92 -> MainThread.stop @14:43:57.88 UserAbort in preston -- User Abort!! @14:44:01.33 -> MainThread.exit @14:44:01.36
SamplePump.stop # @14:44:01.68 SamplePump.stop @14:44:01.72 Intake.close @14:44:01.82 Exhaust.close @14:44:01.99 SamplePump.stop @14:44:03.37 Intake.close @14:44:03.60 Exhaust.close @14:44:03.68 ThreadError in closeExhaust -- deferring killed thread @14:44:03.71 until Exhaust finishes closing @14:44:03.76 Session Ended @14:44:03.97 Abs.stop @14:44:14.32PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:44:16.14 >BREAK< ESP3 Gateway v1.44 for core bus @14:44:16.17 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:44:19.99 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:44:20.76 Sea pressure (Abs) tare is 6% of full scale @14:44:26.24 Polling Can every 2:00 @14:44:26.34 Waiting for espclients... @14:44:27.48 Session Begun @14:44:28.96 -> Power.sampler :off @14:44:28.98 Gate.power :sampler,:OFF false @14:44:29.33 I2C::UnexpectedReply in core -- I2C::Servo::On3Reply[6E:47->20] pressureOutOfBounds with status: @14:44:34.03 -> Power.sampler :off @14:44:34.06 Gate.power :sampler,:OFF false @14:44:44.32 -> ESP.ready! @14:44:44.52 Gate.power :sampler,:OFF,limit to 3500mA @14:44:44.54 Gate.power :skimmer,:OFF,limit to 3500mA @14:44:44.58 Gate.power :analytic1,:ON,limit to 4000mA @14:44:45.32 Plunge.configure :default @14:44:45.41 Plunge.hold @14:44:45.51 Plunge.configure :default @14:44:45.68 SPR Module 1, FW Rev. B, FPGA Rev. C @14:44:45.76 SamplePump.configure :default @14:44:45.94 Toroid.configure :default @14:44:46.02 Toroid.hold @14:44:46.13 Toroid.configure :default @14:44:46.34 Carousel.configure :default @14:44:46.43 Carousel.hold @14:44:46.53 Carousel.configure :default @14:44:46.70 Abs.configure :default @14:44:46.88 PhaseSeparator.configure :default @14:44:47.05 Archive.configure :default @14:44:47.59 Archive.hold @14:44:47.69 Archive.configure :default @14:44:47.88 Air.configure :default @14:44:47.96 Air.hold @14:44:48.06 Air.configure :default @14:44:48.20 Lysis1.configure :default @14:44:48.31 Lysis1.hold @14:44:48.42 Lysis1.configure :default @14:44:48.57 Lysis2.configure :default @14:44:48.65 Lysis2.hold @14:44:48.75 Lysis2.configure :default @14:44:48.92 PinPort.configure :default @14:44:49.00 PinPort.hold @14:44:49.10 PinPort.configure :default @14:44:49.19 All elf controllers running firmware version 4.94 @14:44:49.96 Toroid.seek :clear @14:44:50.17 Air.seek :clear @14:44:54.93 Lysis1.seek :clear @14:44:59.00 Lysis2.seek :clear @14:44:59.12 Archive.seek :clear @14:44:59.24 Carousel.in :default @14:44:59.38 Carousel.home.seek 42 Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) at 29.13ml Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.12psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @14:45:11.36 -> PhytoSampler.goalVolume=100 100 @14:45:20.80 -> start {fire :lyseHold_phyto} # @14:45:21.77 Toroid.seek :clear @14:45:21.99 Air.seek :clear @14:45:22.11 Lysis1.seek :clear @14:45:22.24 Lysis2.seek :clear @14:45:22.42 Archive.seek :clear @14:45:22.54 Carousel.in :default @14:45:22.69 Carousel.home.seek 42 @14:45:23.06 Selecting Cartridge 42 @14:45:23.71 Gate.power :heat,:OFF @14:45:23.73 Gate.power :cartridge,:OFF @14:45:23.85 Carousel.in :default @14:45:24.00 Carousel.home.seek 42 @14:45:24.71 Plunge.in :unlocking @14:45:24.80 Plunge.seek :clear @14:45:25.84 Carousel.seek 42 @14:45:26.15 Carousel.reconfigure :align @14:45:26.34 Lysis1.in :fast @14:45:26.43 Lysis1.seek :locked @14:45:29.09 Plunge.in :default @14:45:29.21 Plunge.seek :locked @14:45:29.37 Air.in :fast @14:45:29.46 Air.seek :locked @14:45:30.55 Carousel.reconfigure :locked @14:45:30.73 Powering cartridge bus @14:45:34.40 Gate.setIR 1750,5000,0,6 @14:45:34.53 Gate.setIR 0,5000,0,6 @14:45:34.62 PuckHeater.configure :default @14:45:34.72 Gate.power :heat,:ON,limit to 5000mA @14:45:34.98 Mounted lysis Cartridge board#J60008 @14:45:35.04 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:45:35.39 Preparing to filter lyseHold_phyto cartridge in slot 42 @14:45:35.66 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @14:45:42.33 Intake Head Pressure tare is 0% of full scale at 25C (drifted 1%) @14:45:42.35 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @14:45:42.41 PhaseSeparator.in :filling @14:45:42.49 PhaseSeparator.hold @14:45:42.59 Gate.power :sampler,:ON @14:45:51.32 Head pressure reached 2.47psi in 9 seconds @14:45:51.37 Air.in :default @14:45:51.53 Air.seek :sealed @14:45:51.59 Priming sample loop w/200ml, bypass w/10ml @14:45:51.65 SamplePump.setPosition! 0ml @14:45:51.76 Exhaust.close @14:45:51.89 Bypass.open @14:45:54.16 Intake.open @14:45:55.86 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:45:58.48 Vacuum tare is 54% of full scale at 25C (drifted 2%) @14:45:58.66 Abs.in :default @14:45:58.74 Abs.hold @14:45:58.84 PhaseSeparator.in :full @14:45:58.92 PhaseSeparator.hold @14:45:59.18 Waiting for sampler to finish priming... @14:45:59.20 delayUntil "14:46:42.63" @14:46:42.69 SamplePump.in :start @14:46:42.79 SamplePump.seek 10ml,1:10 @14:46:43.39 SamplePump.configure :prime @14:46:56.38 Abs.stop @14:46:56.46 PhaseSeparator.stop @14:46:56.54 SamplePump.setPosition! 0ml @14:46:56.63 Exhaust.open @14:46:58.85 Bypass.close @14:47:01.23 Abs.in :default @14:47:01.32 Abs.hold @14:47:01.43 PhaseSeparator.hold @14:47:01.79 SamplePump.in :start @14:47:01.89 SamplePump.seek 200ml,13:50 @14:47:02.47 SamplePump.configure :prime @14:51:24.71 Abs.stop @14:51:24.79 PhaseSeparator.stop @14:51:24.89 Bypass.open @14:51:26.39 Intake.close @14:51:28.14 Toroid.in :hold @14:51:28.23 Toroid.seek :crack @14:51:30.78 Toroid.seek :cartridge @14:51:32.93 Toroid.in :default @14:51:33.02 Toroid.seek :clear @14:51:37.04 Pressurizing cartridge to 14psi above 15.6psia @14:51:37.06 SamplePump.setPosition! 0ml @14:51:37.15 Intake.open @14:51:38.71 Bypass.close @14:51:40.30 Exhaust.close @14:51:41.97 Abs.in :default @14:51:42.05 Abs.hold @14:51:42.16 PhaseSeparator.hold @14:51:42.91 SamplePump.in :charge @14:51:43.10 SamplePump.seek 2.3ml,38 seconds @14:51:56.62 Abs.stop @14:51:56.71 PhaseSeparator.stop @14:51:56.80 Intake.close @14:51:58.50 Pressurized cartridge with 0.754ml to 14.3psi @14:51:58.52 Isolated @27.0psia (11.3psi) @14:51:58.58 Verifying cartridge holds at least 0.65..0.85 of 27.0psia @14:51:58.64 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @14:52:03.26 Cartridge pressure fell 2% to 26.4psia @14:52:03.38 Sampling 100.0ml @14:52:03.40 SamplePump.setPosition! 0ml @14:52:03.50 Exhaust.open @14:52:04.98 Intake.open @14:52:06.76 Abs.in :default @14:52:06.85 Abs.hold @14:52:06.95 PhaseSeparator.hold @14:52:07.31 SamplePump.in :start @14:52:07.42 SamplePump.seek 100ml,6:04 @14:52:08.00 SamplePump.configure :sample @14:52:10.43 Cartridge 42 filtering 2/100ml at 0.76ml/s with 9.4psi vacuum @14:53:10.49 Cartridge 42 filtering 45/100ml at 0.72ml/s with 13.4psi vacuum @14:53:59.39 Session Begun @14:54:01.32 -> Head.psi 2.28064089991342 @14:54:10.49 Cartridge 42 filtering 88/100ml at 0.72ml/s with 12.6psi vacuum @14:54:10.58 -> Head.psi 2.14429368394768 @14:54:27.49 Abs.stop @14:54:27.59 PhaseSeparator.stop @14:54:27.73 Intake.close @14:54:27.79 Exhaust.close @14:54:28.09 Toroid.in :hold @14:54:28.22 Toroid.seek :bypass @14:54:31.89 Toroid.in :default @14:54:32.01 Toroid.seek :clear @14:54:35.63 Sampled 100.0ml @14:54:36.29 Handoff.select :waste @14:54:36.48 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:54:38.80 Gate.power :sampler,:OFF @14:54:43.11 Syringe tare is 51% of full scale at 25C (drifted -0%) @14:54:43.17 PinPort.in :locking @14:54:43.28 PinPort.seek :locked @14:54:44.16 Archive.in :fast @14:54:44.24 Lysis2.in :fast @14:54:44.37 Lysis1.seek 1250uL @14:54:44.52 Archive.seek :empty @14:54:44.69 Lysis2.seek :empty @14:54:53.26 Evacuating puck with filter bubble point of 20psi @14:54:53.32 PinPort.seek :locked @14:54:53.45 Handoff.select :waste @14:54:53.49 Plunge.seek :puckBottom @14:54:54.87 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:55:01.47 Air syringe pumping up to 1 strokes to reach 4psi @14:55:01.49 Air stroke #1 of 1 @14:55:01.67 Air.in :pressurize @14:55:01.76 Air.seek :pressurized @14:55:07.05 -0.3psi after pressurize @14:55:07.08 Air.in :compress @14:55:07.21 Air.seek :evac @14:55:13.86 1.6psi after compress @14:55:25.46 0.2psi after compress (stable) @14:55:25.48 Air.in :default @14:55:25.61 Air.hold @14:55:26.44 at 0.1psi Opened to waste @14:55:26.46 Handoff.select :blocked @14:55:26.67 Plunge.seek :puckTop @14:55:27.59 Air syringe pumping up to 8 strokes to reach 20psi @14:55:27.61 Air stroke #1 of 8 @14:55:27.78 Air.in :compress @14:55:27.89 Air.seek :evac @14:55:28.08 1.2psi after compress @14:55:38.68 1.7psi after compress (stable) @14:55:38.71 Air.in :gulp @14:55:38.84 Air.seek :topVent @14:55:45.35 1.9psi after gulp @14:55:50.95 1.7psi after gulp (stable) @14:55:50.96 Air stroke #2 of 8 @14:55:51.13 Air.in :pressurize @14:55:51.24 Air.seek :pressurized @14:55:57.67 1.6psi after pressurize @14:55:57.70 Air.in :compress @14:55:57.84 Air.seek :evac @14:56:04.51 13.5psi after compress @14:56:16.11 16.1psi after compress (stable) @14:56:16.14 Air.in :gulp @14:56:16.31 Air.seek :topVent @14:56:22.83 16.2psi after gulp @14:56:28.92 16.3psi after gulp (stable) @14:56:28.93 Air stroke #3 of 8 @14:56:29.11 Air.in :pressurize @14:56:29.21 Air.seek :pressurized @14:56:35.63 16.0psi after pressurize @14:56:35.66 Air.in :compress @14:56:35.80 Air.seek :evac @14:56:40.62 Reached 20.4 of 20.0psi after pumping 5215uL (2.94 strokes) @14:56:40.64 Air.in :default @14:56:40.77 Air.hold @14:56:40.90 Plunge.seek :puckBottom @14:56:41.81 delay 3 seconds @14:56:44.84 Handoff.select :waste @14:56:45.01 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @14:56:54.61 delay 1:00 @14:57:54.64 Handoff.select :blocked @14:57:54.83 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @14:58:17.42 Handoff.select :waste @14:58:17.59 0.3psi at evacEnd @14:58:17.62 Plunge.seek :pTop @14:58:18.55 delay 5 seconds @14:58:23.59 Air.in :glide @14:58:23.68 Air.seek :fullVent @14:58:26.49 delay 2 seconds @14:58:28.52 Plunge.seek :pBottom @14:58:29.43 Seawater evacuated, Pressure above/below filter released. @14:58:29.46 Plunge.seek :locked @14:58:30.84 Handoff.select :waste @14:58:30.98 Lysis1.in :slow @14:58:31.12 Lysis1.seek 950uL @14:58:44.67 -> can! @14:58:44.69 Polling Can every 2:00 @14:58:45.23 delay 5 seconds Can@14:58:45, 25.4C, 45% humidity, 14.7psia, 14.843V, 1.275A, 0.915Ah, 18.92W @14:58:50.26 Handoff.select :blocked @14:58:50.49 Plunge.seek :puckTop @14:58:51.50 Lysis1.seek 350uL @14:59:12.39 -> Cart.standards @14:59:12.45 NoMethodError in preston -- undefined method `standards' for Cartridge @14:59:14.71 -> Cart.standard [] @14:59:19.52 delay 3 seconds @14:59:22.62 Air.in mix with 2.79934 parts Lysis1 @14:59:23.44 Air.seek :evac @14:59:23.53 Lysis1.seek 190uL @14:59:31.23 Air.in :fast @14:59:31.34 Air.seek :full @14:59:37.87 Lysis1.seek :empty @14:59:47.03 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @24.2psi @14:59:47.06 Plunge.seek :puckBottom @14:59:48.13 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @21.3psi @14:59:48.16 Polling Can every 2:00 @14:59:48.74 PuckHeater.configure :default #ambient 25.4C @14:59:48.83 PuckHeater.seek 81.5C @15:01:19.71 PuckHeater.hold 2:00,81.5C @15:01:44.64 Session Begun @15:01:47.29 -> slots {[4,2] => { Type: :microSPR_phyto, State: :reserved, was: :dry}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, 40 => { Type: :lyseHold_phyto, State: :dry}, 42 => { Type: :lyseHold_phyto, State: :lysing, filtered: 100.0}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @15:03:19.30 Polling Can every 2:00 @15:03:19.85 PuckHeater.configure :default #ambient 25.4C @15:03:19.98 PuckHeater.seek 80.5C @15:03:20.20 PuckHeater.hold 2:00,80.5C @15:03:21.90 -> slot [4,2] =>[:microSPR_phyto,, :standard, {lysed:1000, lysed2:1000}] @15:03:21.95 SyntaxError in bill -- compile error bill:2: syntax error, unexpected ',', expecting ']' slot [4,2] =>[:microSPR_phyto,, :standard, {lysed:1000, lysed2:1000}] ^ @15:03:45.31 -> slot [4,2] =>[:microSPR_phyto, :standard, {lysed:1000, lysed2:1000}] {[4,2] => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @15:05:19.85 Polling Can every 2:00 @15:05:20.40 PuckHeater.configure :default #ambient 25.2C @15:05:20.53 PuckHeater.hold 8:00,79.4C @15:05:26.83 -> slot {[4,2] => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, 40 => { Type: :lyseHold_phyto, State: :dry}, 42 => { Type: :lyseHold_phyto, State: :lysing, filtered: 100.0}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @15:10:22.55 PuckHeater.configure :default #ambient 24.5C @15:10:22.69 PuckHeater.hold 2:58.01,79.5C @15:13:20.07 Lysate cooked @27.3psi @15:13:20.10 Plunge.seek :puckTop @15:13:21.09 Air syringe pumping up to 5 strokes to reach 40psi @15:13:21.10 Air stroke #1 of 5 @15:13:21.31 Air.in :pressurize @15:13:21.43 Air.seek :pressurized @15:13:27.88 24.7psi after pressurize @15:13:27.91 Air.in :compress @15:13:28.07 Air.seek :evac @15:13:32.80 Reached 41.6 of 40.0psi after pumping 1662uL (0.94 strokes) @15:13:32.83 Air.in :default @15:13:33.01 Air.hold @15:13:33.16 delay 2 seconds @15:13:35.19 Air.in :fast @15:13:35.31 Air.seek :full @15:13:41.47 Plunge.seek :puckBottom @15:13:42.49 Lysis1.seek 138uL @15:13:49.10 delay 2 seconds @15:13:51.13 Plunge.seek :puckTop @15:13:52.06 Lysis1.seek :empty @15:13:58.69 Plunge.seek :puckBottom @15:14:00.47 Lysis1.in :extract @15:14:00.67 Lysis1.seek 675uL,nil @15:14:03.68 Air.in :pressurize @15:14:03.83 Air.seek :pressurized @15:14:04.21 Syringe::LysatePlungerStuck in PROCESSING -- at < 348uL during Lysis Syringe #1 (LyS1) move @15:14:04.34 Lysis1.in :default @15:14:04.46 Lysis1.seek 318uL @15:14:10.36 12.4psi after pressurize @15:14:10.43 Air.in :compressLysate @15:14:10.59 Air.seek :evac @15:14:22.01 Reached 15.7 of 15.5psi after pumping 1566uL (0.88 strokes) @15:14:22.03 Air.in :default @15:14:22.20 Air.hold @15:14:22.43 Lysis1.in :extract @15:14:22.65 Lysis1.seek 675uL,nil @15:14:24.05 Air.in :compressLysate @15:14:24.16 Air.seek :evac @15:14:24.51 Syringe::LysatePlungerStuck in PROCESSING -- at < 417uL during Lysis Syringe #1 (LyS1) move @15:14:24.59 Lysis1.in :default @15:14:24.72 Lysis1.seek 387uL @15:14:25.84 Reached 16.1 of 15.5psi after pumping 1586uL (0.89 strokes) @15:14:25.87 Air.in :default @15:14:26.06 Air.hold @15:14:26.48 Lysis1.in :extract @15:14:26.59 Lysis1.seek 675uL,nil @15:14:28.34 Air.in :compressLysate @15:14:28.57 Air.seek :evac @15:14:29.11 Syringe::LysatePlungerStuck in PROCESSING -- at < 579uL during Lysis Syringe #1 (LyS1) move @15:14:29.19 Lysis1.in :default @15:14:29.32 Lysis1.seek 549uL @15:14:32.89 Reached 15.6 of 15.5psi after pumping 1643uL (0.93 strokes) @15:14:32.92 Air.in :default @15:14:33.10 Air.hold @15:14:33.31 Lysis1.in :extract @15:14:33.47 Lysis1.seek 675uL,nil @15:14:35.47 Air.in :compressLysate @15:14:35.63 Air.seek :evac @15:14:35.82 Air.in :default @15:14:35.91 Air.hold @15:14:36.25 Pulled 675uL lysate @14.0psi @15:14:36.54 Lysis1.seek 800uL,nil @15:14:36.87 Air.in :compressLysate @15:14:37.02 Air.seek :evac @15:14:43.82 Air.in :default @15:14:43.94 Air.hold @15:14:44.25 ThreadError in PROCESSING -- RecoveringLysate thread died holding # @15:14:44.52 Air.in :glide @15:14:44.65 Air.seek :fullVent @15:14:46.72 Handoff.select :waste @15:14:46.91 Recovered 800uL lysate @14.5psi @15:14:46.94 delay 3 seconds @15:14:50.06 After 3 delay Recovered 800uL lysate @0.3psi @15:14:50.40 Handoff.select :close @15:14:50.62 Gate.power :heat,:OFF @15:14:50.64 Gate.power :cartridge,:OFF @15:14:50.85 PinPort.in :unlocking @15:14:50.97 PinPort.seek :clear @15:14:51.86 Carousel.in :default @15:14:52.04 Carousel.home.seek 42 @15:14:52.47 Plunge.seek :locked @15:14:53.93 Archive.in :unlocking @15:14:54.14 Archive.seek :clear @15:14:54.34 Air.in :unlocking @15:14:54.56 Air.seek :clear @15:14:54.68 Lysis1.in :unlocking @15:14:54.91 Lysis1.seek :clear @15:14:55.08 Lysis2.in :unlocking @15:14:55.21 Lysis2.seek :clear @15:15:00.09 Gate.power :motor,:OFF @15:15:00.11 Gate.power :analytic1,:OFF @15:15:00.18 Gate.power :core,:OFF @15:15:00.30 Gate.power :main,:OFF @15:15:00.36 Safely stopped and ready to power off @15:15:47.79 -> ESP.ready!; Cart.lock; PhytoSampler.prepareLGcart 0,0,0; require 'testing.rb' @15:15:47.90 Gate.power :sampler,:OFF,limit to 3500mA @15:15:47.92 Gate.power :skimmer,:OFF,limit to 3500mA @15:15:48.02 Gate.power :main,:ON,limit to 5000mA @15:15:48.05 Gate.power :analytic1,:ON,limit to 4000mA @15:15:48.22 Powering core bus @15:15:50.52 SPR Module 1, FW Rev. B, FPGA Rev. C @15:15:51.00 Plunge.configure :default @15:15:51.13 SamplePump.configure :default @15:15:51.28 Toroid.configure :default @15:15:51.43 Carousel.configure :default @15:15:51.57 Abs.configure :default @15:15:51.71 PhaseSeparator.configure :full @15:15:51.85 Archive.configure :default @15:15:52.00 Air.configure :default @15:15:52.12 Lysis1.configure :default @15:15:52.24 Lysis2.configure :default @15:15:52.42 PinPort.configure :default @15:15:52.54 All elf controllers running firmware version 4.94 @15:15:52.58 Gate.power :motor,:ON,limit to 5000mA @15:15:53.07 Plunge.home.jog 0 @15:15:53.35 Toroid.home.jog 0 @15:15:53.94 PinPort.home.jog 0 @15:15:53.99 Lysis2.home.jog 0 @15:15:54.07 Air.home.jog 0 @15:15:54.14 Carousel.home.jog 0 @15:15:54.27 Lysis1.home.jog 0 @15:15:54.42 Archive.home.jog 0 @15:15:55.83 Plunge.in :homing @15:15:55.95 Toroid.in :homing @15:15:56.19 Toroid.jog 1357 @15:15:56.22 Plunge.jog 1492 @15:15:56.63 PinPort.in :homing @15:15:56.67 Lysis2.in :homing @15:15:56.77 Air.in :homing @15:15:56.84 Lysis1.in :homing @15:15:56.94 Archive.in :homing @15:15:57.25 PinPort.jog 1222 @15:15:57.32 Lysis2.jog 1620 @15:15:57.39 Air.jog 1426 @15:15:57.48 Lysis1.jog 1601 @15:15:57.69 Archive.jog 1620 @15:15:58.08 Plunge.setRawPosition! 50 @15:15:58.19 Plunge.seek :home @15:15:58.59 Plunge.in :default @15:15:59.02 Air.setRawPosition! 0 @15:15:59.15 Air.seek :home @15:15:59.32 Lysis1.setRawPosition! 50 @15:15:59.38 Air.in :default @15:15:59.48 Lysis2.setRawPosition! 50 @15:15:59.53 Archive.setRawPosition! 50 @15:15:59.76 Lysis1.seek :home @15:15:59.99 Lysis2.seek :home @15:16:00.02 Archive.seek :home @15:16:00.33 PinPort.setRawPosition! 3150 @15:16:00.38 Lysis1.in :default @15:16:00.57 PinPort.seek :home @15:16:00.77 Archive.in :default @15:16:00.80 Lysis2.in :default @15:16:01.06 Toroid.setRawPosition! 0 @15:16:01.17 Toroid.seek :home @15:16:01.34 Toroid.in :default @15:16:05.02 PinPort.in :default @15:16:05.42 Toroid.seek :clear @15:16:05.63 PinPort.in :unlocking @15:16:05.75 PinPort.seek :clear @15:16:09.08 Air.seek :clear @15:16:09.23 Lysis1.seek :clear @15:16:10.02 Lysis2.seek :clear @15:16:10.88 Archive.seek :clear @15:16:11.72 Carousel.in :default @15:16:11.91 Carousel.home.seek 42 @15:16:12.36 Carousel.reconfigure :align @15:16:12.55 Lysis1.in :fast @15:16:12.66 Lysis1.seek :locked @15:16:15.36 Plunge.seek :locked @15:16:15.60 Air.in :fast @15:16:15.72 Air.seek :locked @15:16:16.85 Carousel.reconfigure :locked @15:16:17.07 Powering cartridge bus @15:16:19.73 Gate.setIR 1750,5000,0,6 @15:16:19.87 Gate.setIR 0,5000,0,6 @15:16:19.99 PuckHeater.configure :default #ambient 24.5C @15:16:20.13 Gate.power :heat,:ON,limit to 5000mA @15:16:20.83 Mounted lysis Cartridge board#J60008 @15:16:20.92 Using default cartridge heat loss curve for HeatingFunctionInCarousel @15:16:20.97 Handoff.select :waste @15:16:21.31 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:16:27.94 Syringe tare is 51% of full scale at 25C (drifted 0%) @15:16:28.01 PinPort.in :locking @15:16:28.13 PinPort.seek :locked @15:16:29.03 Archive.in :fast @15:16:29.10 Lysis2.in :fast @15:16:29.23 Lysis1.seek :empty @15:16:29.37 Archive.seek :empty @15:16:29.60 Lysis2.seek :empty true @15:17:02.17 -> loadLysisBuffer @15:17:02.29 PinPort.seek :locked @15:17:02.47 Handoff.select :waste @15:17:02.55 Plunge.seek :locked @15:17:02.72 Lysis1.in :default @15:17:02.85 Lysis1.seek :empty @15:17:03.00 Lysis2.in :default @15:17:03.11 Lysis2.seek :empty @15:17:03.26 Archive.in :default @15:17:03.38 Archive.seek :empty @15:17:03.54 Lysis2.seek 300uL @15:17:06.69 Hook up Syringe to waste line @15:17:06.76 Waiting for preston to answer "Push return, when ready to load Lysis Buffer manually" @15:17:06.84 Answered Push return, when ready to load Lysis Buffer manually"" @15:17:06.86 delay 3 seconds @15:17:09.95 Lysis1.seek 1300uL @15:17:21.78 Waiting for preston to answer "unhook syringe, tube back into waste container" @15:17:21.86 Answered unhook syringe, tube back into waste container"" @15:17:21.92 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @15:17:51.05 -> SC Storage Carousel (SC) at 42 @15:17:56.20 -> slot 42=>nil {42 => nil} @15:18:02.84 -> slot 42=>:lyseHold_phyto {42 => { Type: :lyseHold_phyto, State: :dry}} @15:18:04.46 -> slots {[4,2] => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, [34,32] => { Type: :archiveHiBiomass_bac, State: :dry}, [52,50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @15:18:06.89 -> Cart.dry [52,50,42,40,36,34,32,24,22,20,18,16] @15:18:12.65 -> Cart.std @15:18:12.74 NoMethodError in preston -- undefined method `std' for Cartridge @15:18:16.85 -> Cart.standard [4,2] @15:19:20.00 -> MainThread.exit @15:19:20.14 Handoff.select :close # @15:19:20.52 Session Ended @15:19:20.57 Session Ended @15:19:20.67 Session Ended @17:18:31.14PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @17:18:34.49 >BREAK< ESP3 Gateway v1.44 for core bus @17:18:34.51 >BREAK< ESP3 Gateway v1.44 for cartridge bus @17:18:37.34 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @17:18:37.88 Sea pressure (Abs) tare is 6% of full scale @17:18:41.88 Polling Can every 2:00 @17:18:41.92 Waiting for espclients... @17:18:43.32 Session Begun @17:22:20.48 -> Power.sampler :on @17:22:20.50 Gate.power :sampler,:ON true @17:22:57.42 -> Power.sampler :off @17:22:57.44 Gate.power :sampler,:OFF false @17:23:04.23 -> ESP.ready! @17:23:04.26 Gate.power :sampler,:OFF,limit to 3500mA @17:23:04.28 Gate.power :skimmer,:OFF,limit to 3500mA @17:23:04.30 Gate.power :main,:ON,limit to 5000mA @17:23:04.33 Gate.power :analytic1,:ON,limit to 4000mA @17:23:04.48 Powering core bus @17:23:06.76 SPR Module 1, FW Rev. B, FPGA Rev. C @17:23:07.19 Plunge.configure :default @17:23:07.27 SamplePump.configure :default @17:23:07.36 Toroid.configure :default @17:23:07.43 Carousel.configure :default @17:23:07.51 Abs.configure :default @17:23:07.59 PhaseSeparator.configure :default @17:23:07.67 Archive.configure :default @17:23:07.75 Air.configure :default @17:23:07.81 Lysis1.configure :default @17:23:07.87 Lysis2.configure :default @17:23:07.94 PinPort.configure :default @17:23:08.00 All elf controllers running firmware version 4.94 @17:23:08.03 Gate.power :motor,:ON,limit to 5000mA @17:23:08.23 Plunge.home.jog 0 @17:23:09.19 PinPort.home.jog 0 @17:23:09.22 Lysis2.home.jog 0 @17:23:09.27 Air.home.jog 0 @17:23:09.29 Archive.home.jog 0 @17:23:09.32 Carousel.home.jog 0 @17:23:09.35 Toroid.home.jog 0 @17:23:09.38 Lysis1.home.jog 0 @17:23:09.97 Plunge.in :homing @17:23:10.26 PinPort.in :homing @17:23:10.28 Lysis2.in :homing @17:23:10.31 Lysis1.in :homing @17:23:10.34 Air.in :homing @17:23:10.37 Archive.in :homing @17:23:10.40 Plunge.jog 593 @17:23:10.44 Toroid.in :homing @17:23:10.63 PinPort.jog 1293 @17:23:10.66 Lysis2.jog 1621 @17:23:10.73 Lysis1.jog 1596 @17:23:10.78 Air.jog 1426 @17:23:10.84 Archive.jog 1611 @17:23:10.88 Toroid.jog 1360 @17:23:11.36 Plunge.setRawPosition! 50 @17:23:11.42 Plunge.seek :home @17:23:11.70 Plunge.in :default @17:23:12.15 Air.setRawPosition! 0 @17:23:12.24 Lysis2.setRawPosition! 50 @17:23:12.27 Lysis1.setRawPosition! 50 @17:23:12.32 Air.seek :home @17:23:12.39 Lysis2.seek :home @17:23:12.47 Lysis1.seek :home @17:23:12.64 Air.in :default @17:23:12.67 Archive.setRawPosition! 50 @17:23:12.77 PinPort.setRawPosition! 3150 @17:23:12.82 Archive.seek :home @17:23:12.92 PinPort.seek :home @17:23:13.04 Lysis2.in :default @17:23:13.07 Lysis1.in :default @17:23:13.19 Archive.in :default @17:23:15.56 Toroid.setRawPosition! 0 @17:23:15.62 Toroid.seek :home @17:23:15.70 Toroid.in :default @17:23:17.30 PinPort.in :default @17:23:17.63 Toroid.seek :clear @17:23:17.76 PinPort.in :unlocking @17:23:17.83 PinPort.seek :clear @17:23:21.28 Air.seek :clear @17:23:21.37 Lysis1.seek :clear @17:23:22.08 Lysis2.seek :clear @17:23:22.87 Archive.seek :clear @17:23:23.65 Carousel.in :default @17:23:23.77 Carousel.home.seek 40 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 40 Abs = Absolute Pressure (Leak) Sensor @16psia PhaseSeparator = Intake Head Pressure @0.12psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:23:29.43 -> Power.sampler :on @17:23:29.46 Gate.power :sampler,:ON true @17:24:10.20 -> Sampler.refill @17:24:10.22 Bypass.open @17:24:11.80 Intake.open @17:24:13.36 SamplePump.setPosition! 0ml @17:24:13.47 SamplePump.seek 30ml @17:24:53.42 Bypass.close @17:24:54.96 Exhaust.open @17:24:56.52 SamplePump.seek 55ml @17:25:29.92 delay 1 second @17:25:30.94 Intake.close @17:25:30.98 Exhaust.close Sample Pump (SP) at 55ml @17:25:41.53 -> PhytoSampler.prime @17:25:41.62 Priming sample loop w/200ml, bypass w/10ml @17:25:41.63 SamplePump.setPosition! 0ml @17:25:41.70 Bypass.open @17:25:43.96 Intake.open @17:25:45.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:25:48.19 Vacuum tare is 53% of full scale at 24C (drifted 2%) @17:25:48.32 Abs.in :default @17:25:48.38 Abs.hold @17:25:48.45 PhaseSeparator.in :full @17:25:48.51 PhaseSeparator.hold @17:25:48.72 SamplePump.in :start @17:25:48.78 SamplePump.seek 10ml,1:10 @17:25:49.41 SamplePump.configure :prime @17:26:02.30 Abs.stop @17:26:02.35 PhaseSeparator.stop @17:26:02.41 SamplePump.setPosition! 0ml @17:26:02.47 Exhaust.open @17:26:04.67 Bypass.close @17:26:06.95 Abs.in :default @17:26:07.01 Abs.hold @17:26:07.09 PhaseSeparator.hold @17:26:07.36 SamplePump.in :start @17:26:07.43 SamplePump.seek 200ml,13:50 @17:26:08.06 SamplePump.configure :prime @17:29:39.41 SamplePump.hold @17:29:39.62 Intake.close @17:29:39.68 Exhaust.close @17:29:41.28 Abs.stop @17:29:41.35 PhaseSeparator.stop @17:29:41.41 UserAbort in presotn -- Sample Pump (SP) seek to 200ml Aborted! @17:29:54.42 -> PhytoSampler.goalVolume = 100 100 @17:30:06.14 -> PhytoSampler.primeVolume = 50 50 @17:30:19.20 -> PhytSampler.bypassVolume @17:30:19.22 NameError in presotn -- uninitialized constant PhytSampler @17:30:27.25 -> PhytoSampler.bypassVolume 10 @17:30:52.20 -> start {fire lysis, archive} # @17:30:52.93 Toroid.seek :clear @17:30:53.10 Air.seek :clear @17:30:53.18 Lysis1.seek :clear @17:30:53.27 Lysis2.seek :clear @17:30:53.36 Archive.seek :clear @17:30:53.44 Carousel.in :default @17:30:53.56 Carousel.home.seek 40 @17:30:53.82 Selecting Cartridge 52 @17:30:54.08 Carousel.seek 52 @17:30:56.85 Carousel.reconfigure :align @17:30:56.96 Lysis1.in :fast @17:30:57.03 Lysis1.seek :locked @17:30:59.62 Plunge.seek :locked @17:30:59.77 Air.in :fast @17:30:59.82 Air.seek :locked @17:31:00.89 Carousel.reconfigure :locked @17:31:01.02 Powering cartridge bus @17:31:03.65 Gate.setIR 1750,5000,0,6 @17:31:03.77 Gate.setIR 0,5000,0,6 @17:31:03.94 PuckHeater.configure :default @17:31:04.01 Gate.power :heat,:ON,limit to 5000mA @17:31:04.21 Mounted lysis Cartridge board#J59976 @17:31:04.26 Using default cartridge heat loss curve for HeatingFunctionInCarousel @17:31:04.53 Preparing to filter microSPR_phyto cartridge in slot 52 @17:31:04.72 Gate.power :sampler,:OFF @17:31:05.11 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @17:31:11.70 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @17:31:11.71 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @17:31:11.76 PhaseSeparator.in :filling @17:31:11.81 PhaseSeparator.hold @17:31:11.88 Gate.power :sampler,:ON @17:31:21.12 Head pressure reached 2.29psi in 10 seconds @17:31:21.15 Air.in :default @17:31:21.23 Bypass.open @17:31:21.31 Air.seek :sealed @17:31:22.83 Toroid.in :hold @17:31:22.89 Toroid.seek :crack @17:31:25.36 Toroid.seek :cartridge @17:31:27.47 Toroid.in :default @17:31:27.54 Toroid.seek :clear @17:31:31.29 Exhaust.open @17:31:32.78 Intake.open @17:31:34.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:31:36.89 Vacuum tare is 53% of full scale at 24C (drifted 2%) @17:31:37.02 Pressurizing cartridge to 14psi above 14.6psia @17:31:37.04 SamplePump.setPosition! 0ml @17:31:37.10 Bypass.close @17:31:38.63 Exhaust.close @17:31:40.21 Abs.in :default @17:31:40.26 Abs.hold @17:31:40.34 PhaseSeparator.in :full @17:31:40.41 PhaseSeparator.hold @17:31:40.60 Waiting for sampler to finish priming... @17:31:40.61 delayUntil "17:32:11.90" @17:32:11.94 SamplePump.in :charge @17:32:12.02 SamplePump.seek 2.3ml,38 seconds @17:32:25.66 Abs.stop @17:32:25.72 PhaseSeparator.stop @17:32:25.78 Intake.close @17:32:27.45 Pressurized cartridge with 0.774ml to 14.3psi @17:32:27.46 Isolated @27.0psia (12.3psi) @17:32:27.48 Verifying cartridge holds at least 0.65..0.85 of 27.0psia @17:32:27.54 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:32:32.13 Cartridge pressure fell 2% to 26.4psia @17:32:32.22 Sampling 100.0ml @17:32:32.23 SamplePump.setPosition! 0ml @17:32:32.30 Exhaust.open @17:32:33.79 Intake.open @17:32:35.94 Abs.in :default @17:32:35.99 Abs.hold @17:32:36.07 PhaseSeparator.hold @17:32:36.38 SamplePump.in :start @17:32:36.46 SamplePump.seek 100ml,6:04 @17:32:37.08 SamplePump.configure :sample @17:32:39.46 Cartridge 52 filtering 2/100ml at 0.64ml/s with 10.7psi vacuum @17:33:39.51 Cartridge 52 filtering 40/100ml at 0.64ml/s with 11.8psi vacuum @17:34:39.53 Cartridge 52 filtering 79/100ml at 0.64ml/s with 8.4psi vacuum @17:35:12.77 Abs.stop @17:35:12.83 PhaseSeparator.stop @17:35:12.91 Intake.close @17:35:12.97 Exhaust.close @17:35:13.15 Toroid.in :hold @17:35:13.22 Toroid.seek :bypass @17:35:16.80 Toroid.in :default @17:35:16.87 Toroid.seek :clear @17:35:20.45 Sampled 100.0ml @17:35:21.98 SPR.command "read_sequences" @17:35:22.57 SPR.command "stats_on" @17:35:22.70 SPR.command "start 0 1" @17:35:23.43 SPR.start /var/log/esp/spr26jul1917h.spr.bz2 @17:35:23.57 Gate.power :sampler,:OFF @17:35:25.42 Handoff.select :waste @17:35:25.58 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:35:32.17 Syringe tare is 51% of full scale at 25C (drifted -0%) @17:35:32.20 PinPort.in :locking @17:35:32.27 PinPort.seek :locked @17:35:33.11 Archive.in :fast @17:35:33.18 Lysis2.in :fast @17:35:33.24 Lysis1.seek 1250uL @17:35:33.34 Archive.seek :empty @17:35:33.44 Lysis2.seek 950uL @17:35:41.89 Evacuating puck with filter bubble point of 20psi @17:35:41.91 PinPort.seek :locked @17:35:42.04 Handoff.select :waste @17:35:42.07 Plunge.seek :puckBottom @17:35:43.39 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:35:51.49 Air syringe pumping up to 1 strokes to reach 4psi @17:35:51.52 Air stroke #1 of 1 @17:35:51.62 Air.in :pressurize @17:35:51.68 Air.seek :pressurized @17:35:56.91 -0.0psi after pressurize @17:35:56.93 Air.in :compress @17:35:57.01 Air.seek :evac @17:36:03.62 1.3psi after compress @17:36:17.19 0.2psi after compress (stable) @17:36:17.20 Air.in :default @17:36:17.27 Air.hold @17:36:17.47 at 0.3psi Opened to waste @17:36:17.48 Handoff.select :blocked @17:36:17.63 Plunge.seek :puckTop @17:36:18.50 Air syringe pumping up to 8 strokes to reach 20psi @17:36:18.51 Air stroke #1 of 8 @17:36:18.62 Air.in :compress @17:36:18.70 Air.seek :evac @17:36:18.85 1.5psi after compress @17:36:29.44 1.7psi after compress (stable) @17:36:29.46 Air.in :gulp @17:36:29.54 Air.seek :topVent @17:36:35.99 1.8psi after gulp @17:36:41.59 1.8psi after gulp (stable) @17:36:41.61 Air stroke #2 of 8 @17:36:41.72 Air.in :pressurize @17:36:41.78 Air.seek :pressurized @17:36:48.15 1.9psi after pressurize @17:36:48.19 Air.in :compress @17:36:48.28 Air.seek :evac @17:36:54.90 13.5psi after compress @17:37:07.48 16.6psi after compress (stable) @17:37:07.50 Air.in :gulp @17:37:07.57 Air.seek :topVent @17:37:14.02 16.7psi after gulp @17:37:19.60 16.9psi after gulp (stable) @17:37:19.61 Air stroke #3 of 8 @17:37:19.72 Air.in :pressurize @17:37:19.78 Air.seek :pressurized @17:37:26.14 16.9psi after pressurize @17:37:26.17 Air.in :compress @17:37:26.24 Air.seek :evac @17:37:31.00 Reached 20.4 of 20.0psi after pumping 5219uL (2.94 strokes) @17:37:31.01 Air.in :default @17:37:31.09 Air.hold @17:37:31.19 Plunge.seek :puckBottom @17:37:32.06 delay 3 seconds @17:37:35.08 Handoff.select :waste @17:37:35.22 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:37:47.82 delay 1:00 @17:38:47.85 Handoff.select :blocked @17:38:47.99 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @17:39:10.56 Handoff.select :waste @17:39:10.69 0.4psi at evacEnd @17:39:10.71 Plunge.seek :pTop @17:39:11.59 delay 5 seconds @17:39:16.61 Air.in :glide @17:39:16.70 Air.seek :fullVent @17:39:19.46 delay 2 seconds @17:39:21.48 Plunge.seek :pBottom @17:39:22.36 Seawater evacuated, Pressure above/below filter released. @17:39:22.39 Plunge.seek :locked @17:39:23.71 Handoff.select :waste @17:39:23.81 Lysis1.in :slow @17:39:23.86 Lysis1.seek 950uL @17:39:37.93 delay 5 seconds @17:39:42.95 Handoff.select :blocked @17:39:43.10 Plunge.seek :puckTop @17:39:44.05 Lysis1.seek 350uL @17:40:12.03 delay 3 seconds @17:40:15.11 Air.in mix with 2.79934 parts Lysis1 @17:40:15.65 Air.seek :evac @17:40:15.70 Lysis1.seek 190uL @17:40:23.36 Air.in :fast @17:40:23.42 Air.seek :full @17:40:29.93 Lysis1.seek :empty @17:40:39.05 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @55.1psi @17:40:39.07 Plunge.seek :puckBottom @17:40:40.07 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @47.2psi @17:40:40.09 Polling Can every 2:00 @17:40:40.64 PuckHeater.configure :default #ambient 25.0C @17:40:40.70 PuckHeater.seek 81.5C @17:42:07.91 PuckHeater.hold 2:00,81.5C @17:44:07.24 Polling Can every 2:00 @17:44:07.42 -> PhytoSampler.xray @17:44:07.78 PuckHeater.configure :default #ambient 25.3C @17:44:07.84 PuckHeater.seek 80.5C @17:44:07.98 PuckHeater.hold 2:00,80.5C @17:45:04.37 -> BACsampler.xray @17:45:39.23 -> PhytoSampler.xray @17:46:07.37 Polling Can every 2:00 @17:46:07.91 PuckHeater.configure :default #ambient 25.3C @17:46:07.97 PuckHeater.hold 8:00,79.4C @17:54:04.70 Lysate cooked @32.2psi @17:54:04.72 Plunge.seek :puckTop @17:54:05.62 Air syringe pumping up to 5 strokes to reach 40psi @17:54:05.63 Air stroke #1 of 5 @17:54:05.73 Air.in :pressurize @17:54:05.80 Air.seek :pressurized @17:54:12.18 30.0psi after pressurize @17:54:12.20 Air.in :compress @17:54:12.28 Air.seek :evac @17:54:17.31 Reached 41.2 of 40.0psi after pumping 1684uL (0.95 strokes) @17:54:17.33 Air.in :default @17:54:17.40 Air.hold @17:54:17.48 delay 2 seconds @17:54:19.50 Air.in :fast @17:54:19.56 Air.seek :full @17:54:25.74 Plunge.seek :puckBottom @17:54:26.71 Lysis1.seek 138uL @17:54:33.27 delay 2 seconds @17:54:35.30 Plunge.seek :puckTop @17:54:36.17 Lysis1.seek :empty @17:54:42.74 Plunge.seek :puckBottom @17:54:43.85 Lysis1.in :extract @17:54:43.93 Lysis1.seek 675uL,nil @17:54:47.01 Air.in :pressurize @17:54:47.07 Air.seek :pressurized @17:54:47.33 Syringe::LysatePlungerStuck in PROCESSING -- at < 339uL during Lysis Syringe #1 (LyS1) move @17:54:47.37 Lysis1.in :default @17:54:47.46 Lysis1.seek 309uL @17:54:49.12 Reached 14.7 of 15.5psi after pumping 417uL (0.23 strokes) @17:54:49.13 Air.in :default @17:54:49.22 Air.hold @17:54:49.47 Air.in :pressurize @17:54:49.52 Air.seek :pressurized @17:54:54.13 14.4psi after pressurize @17:54:54.14 Air.in :compressLysate @17:54:54.22 Air.seek :evac @17:54:54.42 Reached 14.4 of 15.5psi after pumping 1407uL (0.79 strokes) @17:54:54.44 Air.in :default @17:54:54.50 Air.hold @17:54:54.62 Lysis1.in :extract @17:54:54.71 Lysis1.seek 675uL,nil @17:54:54.97 Air.in :compressLysate @17:54:55.08 Air.seek :evac @17:55:07.77 Syringe::LysatePlungerStuck in PROCESSING -- at < 515uL during Lysis Syringe #1 (LyS1) move @17:55:07.81 Lysis1.in :default @17:55:07.90 Lysis1.seek 485uL @17:55:08.88 Reached 15.6 of 15.5psi after pumping 1605uL (0.90 strokes) @17:55:08.90 Air.in :default @17:55:09.00 Air.hold @17:55:09.18 Lysis1.in :extract @17:55:09.31 Lysis1.seek 675uL,nil @17:55:11.27 Air.in :compressLysate @17:55:11.33 Air.seek :evac @17:55:11.57 Syringe::LysatePlungerStuck in PROCESSING -- at < 632uL during Lysis Syringe #1 (LyS1) move @17:55:11.61 Lysis1.in :default @17:55:11.70 Lysis1.seek 602uL @17:55:13.25 Reached 14.9 of 15.5psi after pumping 1630uL (0.92 strokes) @17:55:13.27 Air.in :default @17:55:13.33 Air.hold @17:55:13.99 Lysis1.in :extract @17:55:14.16 Lysis1.seek 675uL,nil @17:55:15.98 Pulled 675uL lysate @13.6psi @17:55:16.14 Lysis1.seek 800uL,nil @17:55:16.33 Air.in :compressLysate @17:55:16.42 Air.seek :evac @17:55:20.77 Reached 15.3 of 15.5psi after pumping 1690uL (0.95 strokes) @17:55:20.79 Air.in :default @17:55:20.86 Air.hold @17:55:21.74 Air.in :compressLysate @17:55:21.83 Air.seek :evac @17:55:22.95 Air.in :default @17:55:23.00 Air.hold @17:55:23.23 Air.in :glide @17:55:23.28 Air.seek :fullVent @17:55:25.69 Handoff.select :waste @17:55:25.82 Recovered 800uL lysate @14.2psi @17:55:25.84 delay 3 seconds @17:55:28.92 After 3 delay Recovered 800uL lysate @0.9psi @17:55:28.94 Plunge.seek :locked @17:55:30.67 Lysis2.in :DAmix @17:55:30.79 Lysis1.in :DAmix @17:55:31.34 Lysis2.seek 700uL @17:55:31.38 Lysis1.seek 550uL @17:56:06.36 delay 5 seconds @17:56:11.38 Handoff.select SPR @17:56:11.52 SPR.command "start 2 14" @17:56:12.07 SPR stabilized @17:56:12.21 delay 20 seconds @17:56:32.22 SPR.command "start 2 0" @17:56:34.89 Waiting up to 11:00 for SPR to enter state 1 @17:58:32.56 SPR.command "hold_on" @17:58:34.47 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:58:35.06 Lysis2.seek 400uL @17:58:35.13 Lysis1.seek 250uL @17:59:17.04 delay 15 seconds @17:59:32.06 SPR.command "hold_off" @17:59:35.13 Waiting up to 1:00:00 for SPR to enter state 7 @18:15:50.21 SPRsummary:12.7RIU,2.51RIU,6.49RIU,3.75RIU,high,high,high after 2563s @18:30:07.14 SPR analysis completed @18:30:11.63 Ended log spr26jul1917h.spr.bz2 @18:30:11.64 SPR.command "stats_off" @18:30:13.00 Selecting Cartridge 34 @18:30:13.15 Gate.power :heat,:OFF @18:30:13.17 Gate.power :cartridge,:OFF @18:30:13.26 PinPort.in :unlocking @18:30:13.32 PinPort.seek :clear @18:30:14.15 Carousel.in :default @18:30:14.26 Carousel.home.seek 52 @18:30:14.58 Archive.in :unlocking @18:30:14.72 Archive.seek :clear @18:30:14.76 Air.in :unlocking @18:30:14.92 Air.seek :clear @18:30:14.97 Lysis2.in :unlocking @18:30:15.08 Lysis2.seek :clear @18:30:19.70 Lysis1.in :unlocking @18:30:19.84 Lysis1.seek :clear @18:30:19.92 Plunge.in :unlocking @18:30:19.99 Plunge.seek :clear @18:30:24.05 Carousel.seek 34 @18:30:28.04 Carousel.reconfigure :align @18:30:28.16 Lysis1.in :fast @18:30:28.22 Lysis1.seek :locked @18:30:28.29 Slide::Warning in core -- Storage Carousel (SC) reseek at 34 + 5 counts @18:30:30.82 Plunge.in :default @18:30:30.87 Plunge.seek :locked @18:30:31.01 Air.in :fast @18:30:31.07 Air.seek :locked @18:30:32.14 Carousel.reconfigure :locked @18:30:32.28 Powering cartridge bus @18:30:34.90 Gate.setIR 1750,5000,0,6 @18:30:35.03 Gate.setIR 0,5000,0,6 @18:30:35.12 Gate.power :cartridge,:OFF @18:30:35.16 Mounted archive Cartridge @18:30:35.42 Preparing to filter archiveHiBiomass_bac cartridge in slot 34 @18:30:35.62 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @18:30:42.23 Intake Head Pressure tare is 0% of full scale at 27C (drifted 1%) @18:30:42.24 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @18:30:42.29 PhaseSeparator.in :filling @18:30:42.68 PhaseSeparator.hold @18:30:42.76 Gate.power :sampler,:ON @18:30:51.58 Head pressure reached 2.14psi in 10 seconds @18:30:51.67 Priming sample loop w/200ml, bypass w/10ml @18:30:51.69 SamplePump.setPosition! 0ml @18:30:51.75 Bypass.open @18:30:54.02 Intake.open @18:30:55.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:30:58.24 Vacuum tare is 54% of full scale at 27C (drifted 2%) @18:30:58.38 Abs.in :default @18:30:58.44 Abs.hold @18:30:58.51 PhaseSeparator.in :full @18:30:58.57 PhaseSeparator.hold @18:30:58.77 Waiting for sampler to finish priming... @18:30:58.78 delayUntil "18:31:42.77" @18:31:42.83 SamplePump.in :start @18:31:42.89 SamplePump.seek 10ml,1:10 @18:31:43.52 SamplePump.configure :prime @18:31:56.41 Abs.stop @18:31:56.46 PhaseSeparator.stop @18:31:56.52 SamplePump.setPosition! 0ml @18:31:56.58 Exhaust.open @18:31:58.78 Bypass.close @18:32:01.06 Abs.in :default @18:32:01.11 Abs.hold @18:32:01.20 PhaseSeparator.hold @18:32:01.47 SamplePump.in :start @18:32:01.54 SamplePump.seek 200ml,13:50 @18:32:02.16 SamplePump.configure :prime @18:36:24.21 Abs.stop @18:36:24.27 PhaseSeparator.stop @18:36:24.34 Bypass.open @18:36:25.84 Intake.close @18:36:27.54 Toroid.in :hold @18:36:27.59 Toroid.seek :crack @18:36:30.06 Toroid.seek :cartridge @18:36:32.19 Toroid.in :default @18:36:32.25 Toroid.seek :clear @18:36:36.18 Pressurizing cartridge to 26psi above 14.8psia @18:36:36.19 SamplePump.setPosition! 0ml @18:36:36.26 Intake.open @18:36:38.22 Bypass.close @18:36:39.75 Exhaust.close @18:36:41.37 Abs.in :default @18:36:41.43 Abs.hold @18:36:41.51 PhaseSeparator.hold @18:36:41.70 SamplePump.in :charge @18:36:41.77 SamplePump.seek 2.3ml,40 seconds @18:36:53.25 Abs.stop @18:36:53.32 PhaseSeparator.stop @18:36:53.39 Intake.close @18:36:55.10 Pressurized cartridge with 0.547ml to 26.6psi @18:36:55.11 Isolated @37.0psia (22.2psi) @18:36:55.15 Verifying cartridge holds at least 0.65..0.85 of 37.0psia @18:36:55.19 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:36:59.77 Cartridge pressure rose 1% to 37.3psia @18:36:59.87 Sampling 1000.0ml @18:36:59.89 SamplePump.setPosition! 0ml @18:36:59.96 Exhaust.open @18:37:01.40 Intake.open @18:37:03.19 Abs.in :default @18:37:03.25 Abs.hold @18:37:03.32 PhaseSeparator.hold @18:37:03.61 SamplePump.in :start @18:37:03.69 SamplePump.seek 1000ml,1:07:10 @18:37:04.31 SamplePump.configure :sample @18:37:06.69 Cartridge 34 filtering 2/1000ml at 0.76ml/s with 13.4psi vacuum @18:38:06.74 Cartridge 34 filtering 48/1000ml at 0.76ml/s with 8.4psi vacuum @18:39:06.74 Cartridge 34 filtering 94/1000ml at 0.76ml/s with 11.9psi vacuum @18:40:06.74 Cartridge 34 filtering 139/1000ml at 0.76ml/s with 5.9psi vacuum 3.9m deep (20.4psia) @18:41:06.74 Cartridge 34 filtering 185/1000ml at 0.76ml/s with 13.5psi vacuum @18:42:06.74 Cartridge 34 filtering 231/1000ml at 0.76ml/s with 12.4psi vacuum @18:43:06.74 Cartridge 34 filtering 277/1000ml at 0.76ml/s with 12.4psi vacuum @18:44:07.11 Cartridge 34 filtering 322/1000ml at 0.76ml/s with 13.9psi vacuum @18:45:06.74 Cartridge 34 filtering 368/1000ml at 0.76ml/s with 11.5psi vacuum @18:46:06.74 Cartridge 34 filtering 414/1000ml at 0.76ml/s with 9.9psi vacuum 4.4m deep (21.2psia) @18:47:06.74 Cartridge 34 filtering 460/1000ml at 0.76ml/s with 9.8psi vacuum @18:48:06.74 Cartridge 34 filtering 505/1000ml at 0.76ml/s with 11.1psi vacuum 4.5m deep (21.4psia) @18:49:06.74 Cartridge 34 filtering 551/1000ml at 0.76ml/s with 7.5psi vacuum 4.2m deep (21.0psia) @18:50:06.74 Cartridge 34 filtering 597/1000ml at 0.76ml/s with 12.7psi vacuum @18:51:06.74 Cartridge 34 filtering 643/1000ml at 0.76ml/s with 8.5psi vacuum @18:52:06.74 Cartridge 34 filtering 688/1000ml at 0.76ml/s with 13.7psi vacuum @18:53:06.74 Cartridge 34 filtering 734/1000ml at 0.76ml/s with 16.0psi vacuum @18:54:06.74 Cartridge 34 filtering 780/1000ml at 0.76ml/s with 11.6psi vacuum @18:55:06.74 Cartridge 34 filtering 826/1000ml at 0.76ml/s with 16.4psi vacuum @18:56:06.74 Cartridge 34 filtering 871/1000ml at 0.76ml/s with 14.8psi vacuum @18:57:06.74 Cartridge 34 filtering 917/1000ml at 0.76ml/s with 16.4psi vacuum @18:58:06.74 Cartridge 34 filtering 963/1000ml at 0.76ml/s with 16.2psi vacuum @18:58:55.34 Abs.stop @18:58:55.38 PhaseSeparator.stop @18:58:55.47 Intake.close @18:58:55.51 Exhaust.close @18:58:55.61 Handoff.select :close @18:58:55.83 Toroid.in :hold @18:58:55.89 Toroid.seek :bypass @18:58:59.48 Toroid.in :default @18:58:59.55 Toroid.seek :clear @18:59:03.13 Sampled 1000.0ml @18:59:03.63 Plunge.seek :pTop @18:59:04.53 Sample loop closed. Ready to Deliver Reagents @18:59:04.55 Air.in :default @18:59:04.63 Air.seek :sealed @18:59:06.25 Gate.power :sampler,:OFF @18:59:07.37 Air.in :slow @18:59:07.44 Air.seek :arEvac @19:00:22.00 delay 1:00 @19:01:22.04 Air.in :default @19:01:22.10 Air.seek :clear @19:01:40.78 RNAlater soak for 10:00 @19:11:40.81 Air.seek :tVent @19:11:43.28 delay 1 second @19:11:44.30 Air.in :halfSpeed @19:11:44.36 Air.seek :arEmpty @19:12:17.03 delay 10 seconds @19:12:27.06 delay 1:00 @19:13:27.08 Plunge.seek :pTop @19:13:27.58 Sample preserved and evacuated @19:13:27.83 Carousel.in :default @19:13:27.94 Carousel.home.seek 34 @19:13:28.28 Plunge.seek :locked @19:13:29.29 Air.in :unlocking @19:13:29.41 Air.seek :clear @19:13:29.45 Lysis1.in :unlocking @19:13:29.62 Lysis1.seek :clear @19:13:34.32 Gate.power :motor,:OFF @19:13:34.33 Gate.power :analytic1,:OFF @19:13:34.36 Gate.power :core,:OFF @19:13:34.38 Gate.power :main,:OFF @19:13:34.41 Safely stopped and ready to power off @19:14:11.37 Session Begun @19:15:03.84 -> slots {[4,2] => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, [50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 41 => { Type: :archive_bac, State: :missing}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100.0, lysed: 800, spr: spr26jul1917h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @19:15:09.70 -> Cart.standard [4,2] @19:15:14.31 -> Cart.reserve 2 1 @19:15:26.71 -> start {runStandard} # @19:15:26.88 Gate.power :sampler,:OFF,limit to 3500mA @19:15:26.93 Gate.power :skimmer,:OFF,limit to 3500mA @19:15:26.97 Gate.power :main,:ON,limit to 5000mA @19:15:27.00 Gate.power :analytic1,:ON,limit to 4000mA @19:15:27.10 Powering core bus @19:15:29.45 SPR Module 1, FW Rev. B, FPGA Rev. C @19:15:29.83 Plunge.configure :default @19:15:29.92 SamplePump.configure :default @19:15:30.01 Toroid.configure :default @19:15:30.10 Carousel.configure :default @19:15:30.19 Abs.configure :default @19:15:30.28 PhaseSeparator.configure :full @19:15:30.37 Archive.configure :default @19:15:30.46 Air.configure :default @19:15:30.53 Lysis1.configure :default @19:15:30.60 Lysis2.configure :default @19:15:30.69 PinPort.configure :default @19:15:30.75 All elf controllers running firmware version 4.94 @19:15:30.78 Gate.power :motor,:ON,limit to 5000mA @19:15:31.16 Plunge.home.jog 0 @19:15:31.30 Toroid.home.jog 0 @19:15:31.39 Carousel.home.jog 0 @19:15:31.59 Archive.home.jog 0 @19:15:31.67 Air.home.jog 0 @19:15:31.83 Lysis1.home.jog 0 @19:15:31.91 Lysis2.home.jog 0 @19:15:32.04 PinPort.home.jog 0 @19:15:32.76 Plunge.in :homing @19:15:32.82 Toroid.in :homing @19:15:33.29 Plunge.jog 1490 @19:15:33.40 Toroid.jog 1365 @19:15:33.62 Lysis2.in :homing @19:15:33.65 Air.in :homing @19:15:33.71 Archive.in :homing @19:15:33.75 Lysis1.in :homing @19:15:33.82 PinPort.in :homing @19:15:34.00 Lysis2.jog 1621 @19:15:34.07 Air.jog 1423 @19:15:34.11 Archive.jog 1607 @19:15:34.17 Lysis1.jog 1594 @19:15:34.22 PinPort.jog 1223 @19:15:34.69 Plunge.setRawPosition! 50 @19:15:34.76 Plunge.seek :home @19:15:35.05 Plunge.in :default @19:15:35.47 Air.setRawPosition! 0 @19:15:35.54 Air.seek :home @19:15:35.68 Lysis1.setRawPosition! 50 @19:15:35.71 Air.in :default @19:15:35.76 Lysis2.setRawPosition! 50 @19:15:35.79 Archive.setRawPosition! 50 @19:15:35.94 Lysis1.seek :home @19:15:36.02 Lysis2.seek :home @19:15:36.10 Archive.seek :home @19:15:36.28 PinPort.setRawPosition! 3150 @19:15:36.36 PinPort.seek :home @19:15:36.51 Lysis1.in :default @19:15:36.54 Archive.in :default @19:15:36.58 Lysis2.in :default @19:15:38.00 Toroid.setRawPosition! 0 @19:15:38.07 Toroid.seek :home @19:15:38.17 Toroid.in :default @19:15:40.76 PinPort.in :default @19:15:41.10 Toroid.seek :clear @19:15:41.24 PinPort.in :unlocking @19:15:41.32 PinPort.seek :clear @19:15:44.75 Air.seek :clear @19:15:44.86 Lysis1.seek :clear @19:15:45.59 Lysis2.seek :clear @19:15:46.39 Archive.seek :clear @19:15:47.18 Carousel.in :default @19:15:47.32 Carousel.home.seek 34 @19:15:47.57 Selecting Cartridge 4 @19:15:47.83 Carousel.seek 4 @19:15:54.29 Carousel.reconfigure :align @19:15:54.41 Lysis1.in :fast @19:15:54.48 Lysis1.seek :locked @19:15:54.55 Slide::Warning in core -- Storage Carousel (SC) reseek at 4 + 4 counts @19:15:57.11 Plunge.seek :locked @19:15:57.60 Air.in :fast @19:15:57.75 Air.seek :locked @19:15:58.83 Carousel.reconfigure :locked @19:15:58.99 Powering cartridge bus @19:16:01.61 Gate.setIR 1750,5000,0,6 @19:16:01.75 Gate.setIR 0,5000,0,6 @19:16:01.83 PuckHeater.configure :default #ambient 25.3C @19:16:01.91 Gate.power :heat,:ON,limit to 5000mA @19:16:02.07 Mounted lysis Cartridge board#J59994 @19:16:02.13 Using default cartridge heat loss curve for HeatingFunctionInCarousel @19:16:03.60 SPR.command "read_sequences" @19:16:04.37 SPR.command "stats_on" @19:16:04.53 SPR.command "start 0 1" @19:16:05.25 SPR.start /var/log/esp/spr26jul1919h.spr.bz2 @19:16:07.22 Waiting for baseline SPR stabilization @19:16:07.24 Handoff.select :waste @19:16:07.41 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:16:14.02 Syringe tare is 50% of full scale at 28C (drifted -0%) @19:16:14.04 PinPort.in :locking @19:16:14.14 PinPort.seek :locked @19:16:15.00 Archive.in :fast @19:16:15.06 Lysis2.in :fast @19:16:15.15 Lysis1.seek 1000uL @19:16:15.22 Archive.seek :empty @19:16:15.34 Lysis2.seek 1000uL @19:16:23.81 delayUntil "19:26:06.79" @19:16:32.48 -> exit @19:16:32.52 Session Ended @19:26:06.80 Lysis2.in :DAmix @19:26:06.91 Lysis1.in :DAmix @19:26:07.31 Lysis2.seek 750uL @19:26:07.39 Lysis1.seek 750uL @19:26:42.35 delay 5 seconds @19:26:47.38 Handoff.select SPR @19:26:47.51 SPR.command "start 2 14" @19:26:48.51 SPR stabilized @19:26:48.65 delay 20 seconds @19:27:08.67 SPR.command "start 2 0" @19:27:11.85 Waiting up to 11:00 for SPR to enter state 1 @19:29:09.96 SPR.command "hold_on" @19:29:11.34 SPR Dilution 1 of 3 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:29:11.87 Lysis2.seek 450uL @19:29:11.91 Lysis1.seek 450uL @19:29:53.84 delay 15 seconds @19:30:08.85 SPR.command "hold_off" @19:30:12.00 Waiting up to 1:00:00 for SPR to enter state 7 @19:46:27.60 SPRsummary:8.59RIU,6.61RIU,5.68RIU,-0.109RIU after 1924s @20:00:44.64 delay 20 seconds @20:01:04.66 SPR.command "start 2 0" @20:01:07.79 Waiting up to 11:00 for SPR to enter state 1 @20:03:05.46 SPR.command "hold_on" @20:03:06.85 SPR Dilution 2 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:03:07.42 Lysis2.seek 300uL @20:03:07.47 Lysis1.seek 300uL @20:03:28.51 delay 15 seconds @20:03:43.53 SPR.command "hold_off" @20:03:46.69 Waiting up to 1:00:00 for SPR to enter state 7 @20:34:19.33 delay 20 seconds @20:34:39.35 SPR.command "start 2 0" @20:34:42.48 Waiting up to 11:00 for SPR to enter state 1 @20:36:40.16 SPR.command "hold_on" @20:36:41.54 SPR Dilution 3 of 3 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:36:42.09 Lysis2.seek 150uL @20:36:42.13 Lysis1.seek 150uL @20:37:03.18 delay 15 seconds @20:37:18.20 SPR.command "hold_off" @20:37:21.38 Waiting up to 1:00:00 for SPR to enter state 7 @21:07:53.92 SPR analysis completed @21:08:05.44 Ended log spr26jul1919h.spr.bz2 @21:08:05.46 SPR.command "stats_off" @21:11:54.22 Session Begun @21:12:00.70 -> slots {2 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr26jul1919h.spr.bz2}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, [50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 41 => { Type: :archive_bac, State: :missing}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100.0, lysed: 800, spr: spr26jul1917h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @21:12:22.22 -> progress >>> Thread[:MAIN] is sleeping <<< @17:18:41 Waiting for espclients... >>> Thread["presotn"] is sleeping <<< @17:25:41 Priming sample loop w/200ml, bypass w/10ml @17:25:45 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:29:39 Sample Pump (SP) seek to 200ml Aborted! @21:12:44.36 -> SPR # @21:12:50.46 -> SPR.status @21:12:50.49 NoMethodError in preston -- undefined method `status' for SPR:AnalyticModule::SPR @21:22:45.84 Session Begun @21:22:48.30 -> slot {2 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr26jul1919h.spr.bz2}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0}, [50,36] => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry}, 41 => { Type: :archive_bac, State: :missing}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100.0, lysed: 800, spr: spr26jul1917h.spr.bz2}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @21:33:48.47 -> can! @21:33:48.50 Polling Can every 2:00 Can@21:33:49, 29.0C, 44% humidity, 14.7psia, 14.949V, 0.566A, 3.546Ah, 8.46W @21:34:10.42 -> can! @21:34:10.48 Polling Can every 2:00 Can@21:34:11, 29.0C, 44% humidity, 14.7psia, 14.947V, 0.568A, 3.550Ah, 8.49W @21:36:20.46 -> MainThread.exit @21:36:20.56 Handoff.select :close # @21:36:20.80 Session Ended @21:36:20.85 Session Ended @21:36:20.89 Session Ended @21:44:38.66PDT19-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @21:44:40.02 >BREAK< ESP3 Gateway v1.44 for core bus @21:44:40.04 >BREAK< ESP3 Gateway v1.44 for cartridge bus @21:44:42.80 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @21:44:43.33 Sea pressure (Abs) tare is 6% of full scale @21:44:47.32 Polling Can every 2:00 @21:44:47.36 Loading /home/esp/esp2/bin/fireAll mission @21:44:47.57 Cartridge group: dry Schedule: [10:00] @21:44:47.60 delayUntil "21:54:47.33" @21:50:06.96 Session Begun @21:50:12.26 -> slot spare:3 {spare: 3} @21:50:25.61 -> Cart.dry [50,42,40,36,32,24,22,20,18,16] @21:50:51.81 -> exit @21:50:51.84 Session Ended @21:54:47.45
Gate.power :sampler,:OFF,limit to 3500mA @21:54:47.47 Gate.power :skimmer,:OFF,limit to 3500mA @21:54:47.48 Gate.power :analytic1,:ON,limit to 4000mA @21:54:48.06 Plunge.configure :default @21:54:48.11 Plunge.hold @21:54:48.16 Plunge.configure :default @21:54:48.28 SamplePump.configure :default @21:54:48.69 SPR Module 1, FW Rev. B, FPGA Rev. C @21:54:48.79
Toroid.configure :default @21:54:48.83 Toroid.hold @21:54:48.89 Toroid.configure :default @21:54:49.00 Carousel.configure :default @21:54:49.04 Carousel.hold @21:54:49.09 Carousel.configure :default @21:54:49.20 Abs.configure :default @21:54:49.31 PhaseSeparator.configure :default @21:54:49.42 Archive.configure :default @21:54:49.46 Archive.hold @21:54:49.51 Archive.configure :default @21:54:49.62 Air.configure :default @21:54:49.66 Air.hold @21:54:49.72 Air.configure :default @21:54:49.80 Lysis1.configure :default @21:54:49.84 Lysis1.hold @21:54:49.89 Lysis1.configure :default @21:54:49.98 Lysis2.configure :default @21:54:50.02 Lysis2.hold @21:54:50.07 Lysis2.configure :default @21:54:50.18 PinPort.configure :default @21:54:50.23 PinPort.hold @21:54:50.28 PinPort.configure :default @21:54:50.32 All elf controllers running firmware version 4.94 @21:54:50.91 Toroid.seek :clear @21:54:51.06
Air.seek :clear @21:54:53.50 Lysis1.seek :clear @21:55:10.81 Lysis2.seek :clear @21:55:28.07 Archive.seek :clear @21:55:46.63 Carousel.in :default @21:55:46.73 Carousel.home.seek 4 @21:55:47.01 Selecting Cartridge 50 @21:55:47.08 Gate.power :heat,:OFF @21:55:47.10 Gate.power :cartridge,:OFF @21:55:47.16 PinPort.in :unlocking @21:55:47.20 PinPort.seek :clear @21:55:48.01 Carousel.in :default @21:55:48.10 Carousel.home.seek 4 @21:55:48.60 Plunge.in :unlocking @21:55:49.02 Plunge.seek :clear @21:55:50.01
Carousel.seek 50 @21:55:59.69 Carousel.reconfigure :align @21:55:59.79 Lysis1.in :fast @21:55:59.83 Lysis1.seek :locked @21:56:02.41 Plunge.in :default @21:56:02.48 Plunge.seek :locked @21:56:02.58 Air.in :fast @21:56:02.62 Air.seek :locked @21:56:03.68
Carousel.reconfigure :locked @21:56:03.80 Powering cartridge bus @21:56:07.41 Gate.setIR 1750,5000,0,6 @21:56:07.53 Gate.setIR 0,5000,0,6 @21:56:07.58 PuckHeater.configure :default @21:56:07.63 Gate.power :heat,:ON,limit to 5000mA @21:56:07.80 Mounted lysis Cartridge board#J59986 @21:56:07.83 Using default cartridge heat loss curve for HeatingFunctionInCarousel @21:56:08.07 Preparing to filter microSPR_phyto cartridge in slot 50 @21:56:08.25 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:56:14.85 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @21:56:14.86 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @21:56:14.88 PhaseSeparator.in :filling @21:56:14.92 PhaseSeparator.hold @21:56:14.98 Gate.power :sampler,:ON @21:56:23.77 Head pressure reached 2.20psi in 9 seconds @21:56:23.79 Air.in :default @21:56:23.88 Priming sample loop w/200ml, bypass w/10ml @21:56:23.91 Air.seek :sealed @21:56:23.93 SamplePump.setPosition! 0ml @21:56:23.99 Exhaust.close @21:56:24.06 Bypass.open @21:56:26.29 Intake.open @21:56:27.88 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:56:30.54 Vacuum tare is 54% of full scale at 29C (drifted 2%) @21:56:30.65 Abs.in :default @21:56:30.69 Abs.hold @21:56:30.75 PhaseSeparator.in :full @21:56:30.79 PhaseSeparator.hold @21:56:30.95 Waiting for sampler to finish priming... @21:56:30.97 delayUntil "21:57:14.99" @21:57:15.04 SamplePump.in :start @21:57:15.09 SamplePump.seek 10ml,1:10 @21:57:15.73 SamplePump.configure :prime @21:57:28.58 Abs.stop @21:57:28.62 PhaseSeparator.stop @21:57:28.65 SamplePump.setPosition! 0ml @21:57:28.69 Exhaust.open @21:57:30.85 Bypass.close @21:57:33.14 Abs.in :default @21:57:33.19 Abs.hold @21:57:33.24 PhaseSeparator.hold @21:57:33.48 SamplePump.in :start @21:57:33.53 SamplePump.seek 200ml,13:50 @21:57:34.17 SamplePump.configure :prime @21:57:39.73 SamplePump.stop @21:57:39.77 Intake.close @21:57:39.81 Exhaust.close @21:57:41.45 Abs.stop @21:57:41.49 PhaseSeparator.stop @21:57:41.69 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 2.2psi vacuum after 4.4ml @21:57:41.71 Retry #1 of 2 @21:57:41.73 Selecting Cartridge 42 @21:57:41.87 Gate.power :heat,:OFF @21:57:41.88 Gate.power :cartridge,:OFF @21:57:41.94 Carousel.in :default @21:57:42.05 Carousel.home.seek 50 @21:57:42.41 Air.in :unlocking @21:57:42.53 Air.seek :clear @21:57:44.08 Lysis1.in :unlocking @21:57:44.20 Lysis1.seek :clear @21:57:44.24 Plunge.in :unlocking @21:57:44.34 Plunge.seek :clear @21:57:44.61 Gate.power :sampler,:OFF @21:57:45.61 Carousel.seek 42 @21:57:47.96 Carousel.reconfigure :align @21:57:48.06 Lysis1.in :fast @21:57:48.11 Lysis1.seek :locked @21:57:50.70 Plunge.in :default @21:57:50.77 Plunge.seek :locked @21:57:50.88 Air.in :fast @21:57:50.93 Air.seek :locked @21:57:51.99 Carousel.reconfigure :locked @21:57:52.12 Powering cartridge bus @21:57:54.75 Gate.setIR 1750,5000,0,6 @21:57:54.87 Gate.setIR 0,5000,0,6 @21:57:54.92 PuckHeater.configure :default @21:57:54.97 Gate.power :heat,:ON,limit to 5000mA @21:57:55.11 Mounted lysis Cartridge board#J60008 @21:57:55.14 Using default cartridge heat loss curve for HeatingFunctionInCarousel @21:57:55.34 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:58:01.95 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @21:58:01.96 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @21:58:01.99 PhaseSeparator.in :filling @21:58:02.03 PhaseSeparator.hold @21:58:02.09 Gate.power :sampler,:ON @21:58:10.72 Head pressure reached 2.31psi in 9 seconds @21:58:10.75 Air.in :default @21:58:10.85 Priming sample loop w/200ml, bypass w/10ml @21:58:10.88 Air.seek :sealed @21:58:10.90 SamplePump.setPosition! 0ml @21:58:10.99 Bypass.open @21:58:13.30 Intake.open @21:58:15.67 Abs.in :default @21:58:15.72 Abs.hold @21:58:15.78 PhaseSeparator.in :full @21:58:15.83 PhaseSeparator.hold @21:58:16.01 Waiting for sampler to finish priming... @21:58:16.03 delayUntil "21:59:02.11" @21:59:02.17 SamplePump.in :start @21:59:02.23 SamplePump.seek 10ml,1:10 @21:59:02.86 SamplePump.configure :prime @21:59:15.71 Abs.stop @21:59:16.15 PhaseSeparator.stop @21:59:16.21 SamplePump.setPosition! 0ml @21:59:16.25 Exhaust.open @21:59:18.43 Bypass.close @21:59:20.76 Abs.in :default @21:59:20.80 Abs.hold @21:59:20.87 PhaseSeparator.hold @21:59:21.13 SamplePump.in :start @21:59:21.19 SamplePump.seek 200ml,13:50 @21:59:21.82 SamplePump.configure :prime @21:59:26.00 SamplePump.stop @21:59:26.06 Intake.close @21:59:26.12 Exhaust.close @21:59:27.70 Abs.stop @21:59:27.74 PhaseSeparator.stop @21:59:27.94 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 2.9psi vacuum after 3.4ml @21:59:27.96 Retry #2 of 2 @21:59:27.98 Selecting Cartridge 40 @21:59:28.12 Gate.power :heat,:OFF @21:59:28.13 Gate.power :cartridge,:OFF @21:59:28.20 Carousel.in :default @21:59:28.31 Carousel.home.seek 42 @21:59:28.67 Air.in :unlocking @21:59:28.77 Air.seek :clear @21:59:30.32 Lysis1.in :unlocking @21:59:30.45 Lysis1.seek :clear @21:59:30.49 Plunge.in :unlocking @21:59:30.58 Plunge.seek :clear @21:59:30.85 Gate.power :sampler,:OFF @21:59:31.83 Carousel.seek 40 @21:59:32.57 Carousel.reconfigure :align @21:59:32.67 Lysis1.in :fast @21:59:32.72 Lysis1.seek :locked @21:59:32.80 Slide::Warning in core -- Storage Carousel (SC) reseek at 40 + 4 counts @21:59:35.31 Plunge.in :default @21:59:35.40 Plunge.seek :locked @21:59:35.49 Air.in :fast @21:59:35.54 Air.seek :locked @21:59:36.60 Carousel.reconfigure :locked @21:59:36.72 Powering cartridge bus @21:59:39.34 Gate.setIR 1750,5000,0,6 @21:59:39.46 Gate.setIR 0,5000,0,6 @21:59:39.52 PuckHeater.configure :default @21:59:39.57 Gate.power :heat,:ON,limit to 5000mA @21:59:40.13 Mounted lysis Cartridge board#J59925 @21:59:40.16 Using default cartridge heat loss curve for HeatingFunctionInCarousel @21:59:40.35 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:59:46.95 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @21:59:46.97 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @21:59:46.99 PhaseSeparator.in :filling @21:59:47.04 PhaseSeparator.hold @21:59:47.10 Gate.power :sampler,:ON @21:59:55.77 Head pressure reached 2.53psi in 9 seconds @21:59:55.80 Air.in :default @21:59:55.89 Priming sample loop w/200ml, bypass w/10ml @21:59:55.92 Air.seek :sealed @21:59:55.94 SamplePump.setPosition! 0ml @21:59:56.02 Bypass.open @21:59:58.37 Intake.open @22:00:00.77 Abs.in :default @22:00:00.81 Abs.hold @22:00:00.87 PhaseSeparator.in :full @22:00:00.92 PhaseSeparator.hold @22:00:01.10 Waiting for sampler to finish priming... @22:00:01.12 delayUntil "22:00:47.11" @22:00:47.17 SamplePump.in :start @22:00:47.22 SamplePump.seek 10ml,1:10 @22:00:47.86 SamplePump.configure :prime @22:01:00.71 Abs.stop @22:01:00.75 PhaseSeparator.stop @22:01:00.79 SamplePump.setPosition! 0ml @22:01:00.84 Exhaust.open @22:01:03.00 Bypass.close @22:01:05.32 Abs.in :default @22:01:05.36 Abs.hold @22:01:05.42 PhaseSeparator.hold @22:01:05.70 SamplePump.in :start @22:01:05.76 SamplePump.seek 200ml,13:50 @22:01:06.39 SamplePump.configure :prime @22:01:11.09 SamplePump.stop @22:01:11.21 Intake.close @22:01:11.25 Exhaust.close @22:01:12.89 Abs.stop @22:01:12.94 PhaseSeparator.stop @22:01:13.13 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 0.5psi vacuum after 3.5ml @22:01:13.15 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 3.49ml" @22:01:16.05 Gate.power :sampler,:OFF @23:08:27.75 Session Begun @23:08:30.50 -> slot {2 => { Type: :microSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 36 => { Type: :microSPR_phyto, State: :dry}, [42,40] => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 41 => { Type: :archive_bac, State: :missing}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :dry, rank: 1}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @23:09:09.26 -> SC 2 =>{:dry, :microSPR_phyto] @23:09:09.28 SyntaxError in bill -- compile error bill:2: syntax error, unexpected ']', expecting '}' SC 2 =>{:dry, :microSPR_phyto] ^ @23:09:25.47 -> SC 2 =>[:dry, :microSPR_phyto] @23:09:25.51 NoMethodError in bill -- undefined method `SC' for CmdServer::Client:Class @23:09:46.76 -> Slot 2 =>[:dry, :microSPR_phyto] @23:09:46.80 Cartridge::TypeUnknown in bill -- {2 => [:dry,:microSPR_phyto]} is not aRNAl_bac, aRNAl_phyto, aRNAl_zoo, archiveHiBiomass_bac, archiveNull_bac, archive_bac, archive_phyto, archive_zoo, blank_bac, blank_phyto, daSPR_bac, daSPR_phyto, daSPR_phyto9, daSPR_zoo, lyseHoldBS_bac, lyseHoldBS_phyto, lyseHoldBS_zoo, lyseHold_bac, lyseHold_phyto, lyseHold_zoo, lyseNull_bac, microSPR_bac, microSPR_phyto, microSPR_zoo or unknown @23:10:17.89 -> slot 2 =>[:dry, :microSPR_phyto] {2 => { Type: :microSPR_phyto, State: :dry}} @23:10:33.08 -> slot {[36,2] => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, [24,22,20,18,16] => { Type: :aRNAl_bac, State: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [42,40] => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 41 => { Type: :archive_bac, State: :missing}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :dry, rank: 1}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @23:28:54.26 Session Ended @01:01:45.95PDT20-Jul-26 Session Begun @01:01:50.79 -> progress >>> Thread[:MAIN] is sleeping <<< @21:44:47 Cartridge group: dry Schedule: [10:00] @21:55:46 Selecting Cartridge 50 >>> Thread[:FILTERING] is suspended <<< @21:56:08 Preparing to filter microSPR_phyto cartridge in slot 50 @21:56:08 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:56:23 Priming sample loop w/200ml, bypass w/10ml @21:56:27 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:57:36 Sample Pump (SP) pressureOutOfBounds at 4.43ml @21:57:41 Selecting Cartridge 42 @21:57:55 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:58:10 Priming sample loop w/200ml, bypass w/10ml @21:59:24 Sample Pump (SP) pressureOutOfBounds at 3.37ml @21:59:27 Selecting Cartridge 40 @21:59:40 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @21:59:55 Priming sample loop w/200ml, bypass w/10ml @22:01:08 Sample Pump (SP) pressureOutOfBounds at 3.49ml @01:02:20.84 -> Head.psu @01:02:20.87 NoMethodError in preston -- undefined method `psu' for PhaseSeparator:PressureSensor @01:02:24.15 -> Head.psi -0.00211664500852384 @01:02:28.92 -> can! @01:02:28.94 Polling Can every 2:00 Can@01:02:29, 28.9C, 45% humidity, 14.7psia, 14.959V, 0.511A, 5.442Ah, 7.65W @01:02:49.93 -> spare {spare: 3} @01:03:33.70 -> Sampler.power :on @01:03:33.72 NoMethodError in preston -- undefined method `power' for Cartridge::Sampler:Class @01:03:44.42 -> Power.sampler :on @01:03:44.44 Gate.power :sampler,:ON true @01:03:49.21 -> Head.psi 0.238828111795106 @01:03:51.36 -> Head.psi 2.48935091710808 @01:03:52.40 -> Head.psi 2.36252860368069 @01:03:53.40 -> Head.psi 2.39463105297664 @01:03:54.39 -> Head.psi 2.44472498484503 @01:03:55.42 -> Head.psi 2.40486150385117 @01:03:56.41 -> Head.psi 2.35847170074769 @01:04:05.16 -> PhytoSampler.prime @01:04:05.26 Priming sample loop w/200ml, bypass w/10ml @01:04:05.27 SamplePump.setPosition! 0ml @01:04:05.34 Bypass.open @01:04:07.64 Intake.open @01:04:09.29 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:04:11.89 Vacuum tare is 54% of full scale at 29C (drifted 2%) @01:04:12.02 Abs.in :default @01:04:12.07 Abs.hold @01:04:12.15 PhaseSeparator.hold @01:04:12.34 Waiting for sampler to finish priming... @01:04:12.35 delayUntil "01:04:44.47" @01:04:44.52 SamplePump.in :start @01:04:44.59 SamplePump.seek 10ml,1:10 @01:04:45.21 SamplePump.configure :prime @01:04:58.08 Abs.stop @01:04:58.13 PhaseSeparator.stop @01:04:58.19 SamplePump.setPosition! 0ml @01:04:58.24 Exhaust.open @01:05:00.43 Bypass.close @01:05:03.23 Abs.in :default @01:05:03.29 Abs.hold @01:05:03.37 PhaseSeparator.hold @01:05:03.64 SamplePump.in :start @01:05:03.70 SamplePump.seek 200ml,13:50 @01:05:04.33 SamplePump.configure :prime @01:07:29.30 Session Begun @01:07:32.14 -> Head.psi 2.11646862143979 @01:07:41.72 -> SP Sample Pump (SP) at 120.34ml @01:08:05.91 -> slot [42,40]=>nil {[42,40] => nil} @01:08:24.62 -> slot [42,40]=>[:lyseHold_phyto, :dry] {[42,40] => { Type: :lyseHold_phyto, State: :dry}} @01:08:48.98 -> SP Sample Pump (SP) at 171.65ml @01:09:12.83 -> SP Sample Pump (SP) at 189.85ml @01:09:15.54 -> Head.psi 2.12546436272602 @01:09:26.27 Abs.stop @01:09:26.34 PhaseSeparator.stop PhytoSampler @01:09:48.10 -> Power.sample.status @01:09:48.22 I2C::Sleepy::Error in preston#2 -- Unknown powered device: sample @01:09:53.90 -> Power.status {analytic1: ON with current limited to 4000mA, cartridge: ON, core: ON, heat: ON with current limited to 5000mA, main: ON with current limited to 5000mA, motor: ON with current limited to 5000mA, sampler: ON with current limited to 3500mA, skimmer: off with current limited to 3500mA} @01:10:00.26 -> Power.sampler :off @01:10:00.28 Gate.power :sampler,:OFF false @01:10:04.22 -> Head.psi -0.00211664500852384 @01:10:32.14 -> MainThread.exit @01:10:32.26 Intake.close @01:10:32.30 Exhaust.close # @01:10:33.93 Session Ended @01:10:33.98 Session Ended @01:10:47.79PDT20-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @01:10:49.10 >BREAK< ESP3 Gateway v1.44 for core bus @01:10:49.12 >BREAK< ESP3 Gateway v1.44 for cartridge bus @01:10:51.77 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @01:10:52.28 Sea pressure (Abs) tare is 6% of full scale @01:10:56.34 Polling Can every 2:00 @01:10:56.37 Loading /home/esp/esp2/bin/fireAll mission @01:10:56.58 Cartridge group: dry Spares: {spare: 3} Schedule: [10:00] @01:10:56.61 delayUntil "01:20:56.35" @01:11:20.13 Session Begun @01:11:23.98 -> MainThread.exit # @01:11:24.05 ThreadError in preston -- killed thread @01:11:24.11 Session Ended @01:11:47.00PDT20-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @01:11:48.31 >BREAK< ESP3 Gateway v1.44 for core bus @01:11:48.33 >BREAK< ESP3 Gateway v1.44 for cartridge bus @01:11:51.00 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @01:11:51.53 Sea pressure (Abs) tare is 6% of full scale @01:11:56.05 Polling Can every 2:00 @01:11:56.08 Loading /home/esp/esp2/bin/fireAll mission @01:11:56.29 Cartridge group: dry Spares: {spare: 3} @01:11:56.48 Gate.power :sampler,:OFF,limit to 3500mA @01:11:56.50 Gate.power :skimmer,:OFF,limit to 3500mA @01:11:56.52 Gate.power :analytic1,:ON,limit to 4000mA @01:11:57.38 SPR Module 1, FW Rev. B, FPGA Rev. C @01:11:57.75 Session Begun @01:11:57.84
Plunge.configure :default @01:11:57.90 Plunge.hold @01:11:57.98 Plunge.configure :default @01:11:58.11 SamplePump.configure :default @01:11:58.59 Toroid.configure :default @01:11:58.66 Toroid.hold @01:11:58.73 Toroid.configure :default @01:11:58.86 Carousel.configure :default @01:11:58.93 Carousel.hold @01:11:59.00 Carousel.configure :default @01:11:59.12 Abs.configure :default @01:11:59.25 PhaseSeparator.configure :default @01:11:59.39 Archive.configure :default @01:11:59.45 Archive.hold @01:11:59.53 Archive.configure :default @01:11:59.66 Air.configure :default @01:11:59.71 Air.hold @01:11:59.78 Air.configure :default @01:11:59.88 Lysis1.configure :default @01:11:59.94 Lysis1.hold @01:12:00.01 Lysis1.configure :default @01:12:00.11 Lysis2.configure :default @01:12:00.18 Lysis2.hold @01:12:00.25 Lysis2.configure :default @01:12:00.39 PinPort.configure :default @01:12:00.44 PinPort.hold @01:12:00.51 PinPort.configure :default @01:12:00.58 All elf controllers running firmware version 4.94 @01:12:01.18 Toroid.seek :clear @01:12:01.35
Air.seek :clear @01:12:06.07 Lysis1.seek :clear @01:12:10.12 Lysis2.seek :clear @01:12:10.21 Archive.seek :clear @01:12:10.29 Carousel.in :default @01:12:10.41 Carousel.home.seek 40 @01:12:10.71 Selecting Cartridge 42 @01:12:10.82 Gate.power :heat,:OFF @01:12:10.83 Gate.power :cartridge,:OFF @01:12:10.92 Carousel.in :default @01:12:11.03 Carousel.home.seek 40 @01:12:11.58 Plunge.in :unlocking @01:12:11.64 Plunge.seek :clear @01:12:12.64
Carousel.seek 42 @01:12:13.70 Carousel.reconfigure :align @01:12:13.81 Lysis1.in :fast @01:12:13.88 Lysis1.seek :locked @01:12:16.54 Plunge.in :default @01:12:16.65 Plunge.seek :locked @01:12:16.75 Air.in :fast @01:12:16.82 Air.seek :locked @01:12:17.89
Carousel.reconfigure :locked @01:12:18.03 Powering cartridge bus @01:12:21.65 Gate.setIR 1750,5000,0,6 @01:12:21.78 Gate.setIR 0,5000,0,6 @01:12:21.85 PuckHeater.configure :default @01:12:21.91 Gate.power :heat,:ON,limit to 5000mA @01:12:22.09 Mounted lysis Cartridge board#J60008 @01:12:22.13 Using default cartridge heat loss curve for HeatingFunctionInCarousel @01:12:22.41 Preparing to filter lyseHold_phyto cartridge in slot 42 @01:12:22.60 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @01:12:29.21 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @01:12:29.22 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @01:12:29.26 PhaseSeparator.in :filling @01:12:29.31 PhaseSeparator.hold @01:12:29.39 Gate.power :sampler,:ON @01:12:37.86 Head pressure reached 2.56psi in 9 seconds @01:12:37.88 Air.in :default @01:12:38.00 Priming sample loop w/200ml, bypass w/10ml @01:12:38.03 Air.seek :sealed @01:12:38.06 SamplePump.setPosition! 0ml @01:12:38.17 Exhaust.close @01:12:38.25 Bypass.open @01:12:40.51 Intake.open @01:12:42.08 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @01:12:44.99 Vacuum tare is 54% of full scale at 29C (drifted 2%) @01:12:45.13 Abs.in :default @01:12:45.19 Abs.hold @01:12:45.27 PhaseSeparator.in :full @01:12:45.32 PhaseSeparator.hold @01:12:45.45 -> slots [24,22,20,18,16]=>:archiveHiBiomass_bac {[24,22,20,18,16] => { Type: :archiveHiBiomass_bac, State: :dry}} @01:12:45.70 Waiting for sampler to finish priming... @01:12:45.71 delayUntil "01:13:29.41" @01:12:53.94 -> Head.psi 2.43418585657342 @01:13:14.24 -> Head.psi 2.5306695915453 @01:13:29.47 SamplePump.in :start @01:13:29.55 SamplePump.seek 10ml,1:10 @01:13:30.16 SamplePump.configure :prime @01:13:43.05 Abs.stop @01:13:43.10 PhaseSeparator.stop @01:13:43.15 SamplePump.setPosition! 0ml @01:13:43.21 Exhaust.open @01:13:45.39 Bypass.close @01:13:47.72 Abs.in :default @01:13:47.78 Abs.hold @01:13:47.86 PhaseSeparator.hold @01:13:48.12 SamplePump.in :start @01:13:48.19 SamplePump.seek 200ml,13:50 @01:13:48.82 SamplePump.configure :prime @01:13:56.57 -> Head.psi 2.32482586446636 @01:14:37.80 -> can! @01:14:37.83 Polling Can every 2:00 Can@01:14:38, 29.1C, 44% humidity, 14.7psia, 14.711V, 1.964A, 5.728Ah, 28.89W @01:15:07.74 -> SP Sample Pump (SP) at 60.49ml @01:15:23.01 -> SP Sample Pump (SP) at 72.14ml @01:15:27.24 -> exit @01:15:27.26 Session Ended @01:16:40.05 SamplePump.stop @01:16:40.10 Intake.close @01:16:40.15 Exhaust.close @01:16:41.85 Abs.stop @01:16:41.89 PhaseSeparator.stop @01:16:42.09 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 1.5psi vacuum after 130.8ml @01:16:42.10 Retry #1 of 2 @01:16:42.13 Selecting Cartridge 40 @01:16:42.26 Gate.power :heat,:OFF @01:16:42.27 Gate.power :cartridge,:OFF @01:16:42.34 Carousel.in :default @01:16:42.44 Carousel.home.seek 42 @01:16:42.80 Air.in :unlocking @01:16:42.90 Air.seek :clear @01:16:44.45 Lysis1.in :unlocking @01:16:44.55 Lysis1.seek :clear @01:16:44.64 Plunge.in :unlocking @01:16:44.69 Plunge.seek :clear @01:16:45.15 Gate.power :sampler,:OFF @01:16:46.09 Carousel.seek 40 @01:16:46.82 Carousel.reconfigure :align @01:16:46.92 Lysis1.in :fast @01:16:46.97 Lysis1.seek :locked @01:16:49.56 Plunge.in :default @01:16:49.65 Plunge.seek :locked @01:16:49.74 Air.in :fast @01:16:49.78 Air.seek :locked @01:16:50.84 Carousel.reconfigure :locked @01:16:50.97 Powering cartridge bus @01:16:53.60 Gate.setIR 1750,5000,0,6 @01:16:53.72 Gate.setIR 0,5000,0,6 @01:16:53.78 PuckHeater.configure :default @01:16:53.83 Gate.power :heat,:ON,limit to 5000mA @01:16:53.97 Mounted lysis Cartridge board#J59925 @01:16:53.99 Using default cartridge heat loss curve for HeatingFunctionInCarousel @01:16:54.20 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @01:17:00.82 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @01:17:00.83 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @01:17:00.86 PhaseSeparator.in :filling @01:17:00.90 PhaseSeparator.hold @01:17:00.96 Gate.power :sampler,:ON @01:17:05.86 Session Begun @01:17:09.43 Head pressure reached 2.58psi in 9 seconds @01:17:09.47 Air.in :default @01:17:09.61 Air.seek :sealed @01:17:09.64 Priming sample loop w/200ml, bypass w/10ml @01:17:09.70 SamplePump.setPosition! 0ml @01:17:09.79 Bypass.open @01:17:11.93 -> Head.psi 2.48710198178652 @01:17:12.18 Intake.open @01:17:14.55 Abs.in :default @01:17:14.61 Abs.hold @01:17:14.70 PhaseSeparator.in :full @01:17:14.77 -> Head.psi @01:17:14.81 PhaseSeparator.hold 2.41107914856371 @01:17:15.56 Waiting for sampler to finish priming... @01:17:15.58 delayUntil "01:18:00.98" @01:17:16.95 -> Head.psi 2.52590714027612 @01:17:22.91 -> can! @01:17:22.93 Polling Can every 2:00 Can@01:17:23, 29.2C, 44% humidity, 14.7psia, 14.820V, 1.421A, 5.810Ah, 21.07W @01:17:38.33 -> SP Sample Pump (SP) at 0ml @01:17:52.48 -> SP Sample Pump (SP) at 0ml @01:17:53.54 -> SP Sample Pump (SP) at 0ml @01:17:58.54 -> Head.psi 2.42554288945529 @01:18:01.04 SamplePump.in :start @01:18:01.12 SamplePump.seek 10ml,1:10 @01:18:01.21 -> Head.psi 2.42342624444676 @01:18:01.73 SamplePump.configure :prime @01:18:03.83 -> Head.psi 2.34211179870264 @01:18:05.32 -> Head.psi 2.36468934546023 @01:18:09.10 -> Sampler.psi 3.84218443073868 @01:18:12.12 -> Sampler.psi 3.76238137895356 @01:18:13.29 -> Sampler.psi 5.2487132184518 @01:18:14.62 Abs.stop @01:18:14.68 PhaseSeparator.stop @01:18:14.74 SamplePump.setPosition! 0ml @01:18:14.80 Exhaust.open @01:18:17.00 Bypass.close @01:18:19.41 Abs.hold @01:18:19.49 PhaseSeparator.hold @01:18:19.78 SamplePump.in :start @01:18:19.85 SamplePump.seek 200ml,13:50 @01:18:20.47 SamplePump.configure :prime @01:18:32.02 -> Head.psi 2.29095954432998 @01:18:33.95 -> Sampler.psi 8.89471514688537 @01:18:40.48 -> Sampler.psi 10.1117116866087 @01:18:41.66 -> Sampler.psi 7.40339561665056 @01:18:42.76 -> Sampler.psi 6.89963885225686 @01:18:43.77 -> Sampler.psi 11.2788313189664 @01:18:44.79 -> Sampler.psi 9.76756102578534 @01:19:09.47 -> loop {puts Sampler.psi; Delay.sleep 1} @01:19:40.88 UserAbort in preston -- User Abort!! @01:19:45.47 -> SP Sample Pump (SP) at 65.13ml @01:19:52.24 -> loop {puts Sampler.psi; Delay.sleep 1} @01:19:59.53 UserAbort in preston -- User Abort!! @01:20:10.06 -> PhytoSampler.primeVac 5..50 @01:20:30.08 -> loop {puts Sampler.psi; Delay.sleep 1} @01:22:03.46 UserAbort in preston -- User Abort!! @01:22:05.80 -> SP Sample Pump (SP) at 172.2ml @01:22:10.21 -> loop {puts Sampler.psi; Delay.sleep 1} @01:22:42.36 Abs.stop @01:22:42.42 PhaseSeparator.stop @01:22:42.50 Bypass.open @01:22:43.99 Intake.close @01:22:45.57 Toroid.in :hold @01:22:45.63 Toroid.seek :crack @01:22:48.10 Toroid.seek :cartridge @01:22:50.23 Toroid.in :default @01:22:50.29 Toroid.seek :clear @01:22:54.30 Pressurizing cartridge to 14psi above 14.1psia @01:22:54.31 SamplePump.setPosition! 0ml @01:22:54.38 Intake.open @01:22:55.93 Bypass.close @01:22:57.39 Exhaust.close @01:22:59.36 Abs.in :default @01:22:59.43 Abs.hold @01:22:59.51 PhaseSeparator.hold @01:22:59.72 SamplePump.in :charge @01:22:59.80 SamplePump.seek 2.3ml,38 seconds @01:23:12.52 Abs.stop @01:23:12.58 PhaseSeparator.stop @01:23:12.63 Intake.close @01:23:14.32 Pressurized cartridge with 0.674ml to 14.1psi @01:23:14.34 Isolated @27.4psia (13.3psi) @01:23:14.37 Verifying cartridge holds at least 0.65..0.85 of 27.4psia @01:23:14.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @01:23:19.05 Sampling 1000.0ml @01:23:19.06 SamplePump.setPosition! 0ml @01:23:19.14 Exhaust.open @01:23:20.62 Intake.open @01:23:22.38 Abs.in :default @01:23:22.45 Abs.hold @01:23:22.59 PhaseSeparator.hold @01:23:22.90 SamplePump.in :start @01:23:22.98 SamplePump.seek 1000ml,56:04 @01:23:23.62 SamplePump.configure :sample @01:23:26.05 Cartridge 40 filtering 2/1000ml at 0.74ml/s with 11.5psi vacuum @01:24:05.62 UserAbort in preston -- User Abort!! @01:24:10.66 -> Head.psi 2.32905915448341 @01:24:18.08 -> exit @01:24:18.10 Session Ended @01:24:26.09 Cartridge 40 filtering 45/1000ml at 0.71ml/s with 12.0psi vacuum @01:24:48.03 Session Begun @01:24:52.61 -> SC Storage Carousel (SC) at 40 @01:24:58.47 -> slot 42=>nil {42 => nil} @01:25:10.36 -> slot 42=>:lyseHold_phyto {42 => { Type: :lyseHold_phyto, State: :dry}} @01:25:14.27 -> Cart.dry [42,36,32,24,22,20,18,16,2,50] @01:25:26.08 Cartridge 40 filtering 87/1000ml at 0.71ml/s with 11.0psi vacuum @01:26:18.58 -> slot 50=>nil {50 => nil} @01:26:26.09 Cartridge 40 filtering 129/1000ml at 0.70ml/s with 7.5psi vacuum @01:26:41.39 -> slot 50=>:microSPR_phyto {50 => { Type: :microSPR_phyto, State: :dry}} @01:26:44.89 -> Cart.dry [50,42,36,32,24,22,20,18,16,2] @01:27:02.13 -> SP Sample Pump (SP) at 153.82ml @01:27:11.51 -> exit @01:27:11.53 Session Ended @01:27:26.08 Cartridge 40 filtering 170/1000ml at 0.68ml/s with 6.9psi vacuum 4.5m deep (21.3psia) @01:28:26.09 Cartridge 40 filtering 215/1000ml at 0.75ml/s with 6.9psi vacuum 5.2m deep (22.4psia) @01:29:26.08 Cartridge 40 filtering 260/1000ml at 0.76ml/s with 9.1psi vacuum @01:30:26.09 Cartridge 40 filtering 306/1000ml at 0.75ml/s with 4.2psi vacuum 6.1m deep (23.7psia) @01:31:26.09 Cartridge 40 filtering 350/1000ml at 0.74ml/s with 8.5psi vacuum 3.9m deep (20.4psia) @01:32:26.09 Cartridge 40 filtering 394/1000ml at 0.74ml/s with 12.3psi vacuum @01:33:26.09 Cartridge 40 filtering 438/1000ml at 0.73ml/s with 5.8psi vacuum 6.0m deep (23.6psia) @01:34:26.09 Cartridge 40 filtering 482/1000ml at 0.73ml/s with 14.9psi vacuum @01:35:26.09 Cartridge 40 filtering 525/1000ml at 0.72ml/s with 11.9psi vacuum @01:36:26.08 Cartridge 40 filtering 568/1000ml at 0.71ml/s with 16.0psi vacuum @01:37:26.49 Cartridge 40 filtering 610/1000ml at 0.70ml/s with 8.2psi vacuum 4.5m deep (21.4psia) @01:38:26.09 Cartridge 40 filtering 651/1000ml at 0.69ml/s with 11.1psi vacuum @01:39:26.09 Cartridge 40 filtering 692/1000ml at 0.68ml/s with 10.1psi vacuum @01:40:26.09 Cartridge 40 filtering 732/1000ml at 0.68ml/s with 6.9psi vacuum 4.0m deep (20.7psia) @01:41:26.09 Cartridge 40 filtering 772/1000ml at 0.67ml/s with 5.7psi vacuum 6.9m deep (24.9psia) @01:42:26.09 Cartridge 40 filtering 812/1000ml at 0.66ml/s with 13.7psi vacuum @01:43:26.10 Cartridge 40 filtering 850/1000ml at 0.64ml/s with 11.5psi vacuum @01:44:26.09 Cartridge 40 filtering 888/1000ml at 0.63ml/s with 11.1psi vacuum @01:45:26.09 Cartridge 40 filtering 926/1000ml at 0.63ml/s with 15.1psi vacuum @01:46:26.09 Cartridge 40 filtering 963/1000ml at 0.62ml/s with 10.5psi vacuum @01:47:25.94 Abs.stop @01:47:25.98 PhaseSeparator.stop @01:47:26.04 Intake.close @01:47:26.09 Exhaust.close @01:47:26.28 Toroid.in :hold @01:47:26.33 Toroid.seek :bypass @01:47:29.91 Toroid.in :default @01:47:29.95 Toroid.seek :clear @01:47:33.51 Sampled 1000.0ml @01:47:33.92 Handoff.select :waste @01:47:34.03 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:47:36.61 Gate.power :sampler,:OFF @01:47:44.13 Syringe tare is 50% of full scale at 29C (drifted -0%) @01:47:44.15 PinPort.in :locking @01:47:44.19 PinPort.seek :locked @01:47:45.00 Archive.in :fast @01:47:45.05 Lysis2.in :fast @01:47:45.14 Lysis1.seek 1250uL @01:47:45.16 Archive.seek :empty @01:47:45.18 Lysis2.seek :empty @01:47:54.03 Evacuating puck with filter bubble point of 20psi @01:47:54.05 PinPort.seek :locked @01:47:54.11 Handoff.select :waste @01:47:54.13 Plunge.seek :puckBottom @01:47:55.42 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @01:48:01.99 Air syringe pumping up to 1 strokes to reach 4psi @01:48:02.00 Air stroke #1 of 1 @01:48:02.06 Air.in :pressurize @01:48:02.11 Air.seek :pressurized @01:48:07.33 -0.1psi after pressurize @01:48:07.34 Air.in :compress @01:48:07.38 Air.seek :evac @01:48:13.97 1.3psi after compress @01:48:14.18 Reached 2.0 of 4.0psi after pumping 1775uL (1.00 strokes) @01:48:14.19 Air.in :default @01:48:14.23 Air.hold @01:48:14.33 Handoff.select :blocked @01:48:14.45 Plunge.seek :puckTop @01:48:15.31 Overpressure in PROCESSING -- Waste port clogged (1.5psi) @01:48:15.32 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 4psi" @03:16:47.93 Session Begun @03:17:02.54 -> Syringe.psi 5.12846410895304 @03:18:01.20 -> Thread[:PROCESSING].resume @03:18:01.23 Resuming Thread (suspended since 01:48:01) # @03:18:01.28 Air syringe pumping up to 1 strokes to reach 4psi @03:18:01.30 Air stroke #1 of 1 @03:18:01.41 Air.in :compress @03:18:01.47 Air.seek :evac @03:18:01.67 Reached 4.9 of 4.0psi after pumping 3551uL (2.00 strokes) @03:18:01.69 Air.in :default @03:18:01.76 Air.hold @03:18:01.89 Handoff.select :blocked @03:18:01.92 Plunge.seek :puckTop @03:18:02.03 Overpressure in PROCESSING -- Waste port clogged (5.0psi) @03:18:02.04 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 4psi" @03:18:18.45 -> Handoff.open @03:18:18.47 Handoff.select :open @03:18:18.50 Valve::Manifold::Error in preston -- Handoff Valve::Manifold has no selection: open @03:18:26.84 -> Handoff.waste @03:18:26.85 Handoff.select :waste Handoff Valve::Manifold selects waste @03:18:32.37 -> Thread[:PROCESSING].resume @03:18:32.41 Resuming Thread (suspended since 01:48:01) @03:18:32.43 # Air syringe pumping up to 1 strokes to reach 4psi @03:18:32.46 Air stroke #1 of 1 @03:18:32.57 Air.in :compress @03:18:32.64 Air.seek :evac @03:18:32.76 0.4psi after compress @03:18:43.33 0.5psi after compress (stable) @03:18:43.35 Air.in :default @03:18:43.42 Air.hold @03:18:43.61 at 0.6psi Opened to waste @03:18:43.63 Handoff.select :blocked @03:18:43.77 Plunge.seek :puckTop @03:18:43.85 Air syringe pumping up to 8 strokes to reach 20psi @03:18:43.86 Air stroke #1 of 8 @03:18:43.96 Air.in :compress @03:18:44.02 Air.seek :evac @03:18:44.15 0.7psi after compress @03:18:54.72 0.6psi after compress (stable) @03:18:54.74 Air.in :gulp @03:18:54.82 Air.seek :topVent @03:19:01.26 0.7psi after gulp @03:19:09.84 0.7psi after gulp (stable) @03:19:09.85 Air stroke #2 of 8 @03:19:09.95 Air.in :pressurize @03:19:10.09 Air.seek :pressurized @03:19:16.46 0.7psi after pressurize @03:19:16.48 Air.in :compress @03:19:16.56 Air.seek :evac @03:19:23.16 12.9psi after compress @03:19:34.73 15.3psi after compress (stable) @03:19:34.75 Air.in :gulp @03:19:34.83 Air.seek :topVent @03:19:41.28 15.3psi after gulp @03:19:52.86 15.4psi after gulp (stable) @03:19:52.87 Air stroke #3 of 8 @03:19:52.96 Air.in :pressurize @03:19:53.02 Air.seek :pressurized @03:19:59.39 15.5psi after pressurize @03:19:59.40 Air.in :compress @03:19:59.48 Air.seek :evac @03:20:04.52 Reached 20.5 of 20.0psi after pumping 5230uL (2.95 strokes) @03:20:04.54 Air.in :default @03:20:04.61 Air.hold @03:20:04.69 Plunge.seek :puckBottom @03:20:05.56 delay 3 seconds @03:20:08.58 Handoff.select :waste @03:20:08.71 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @03:20:21.28 delay 1:00 @03:21:21.30 Handoff.select :blocked @03:21:21.44 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @03:21:44.00 Handoff.select :waste @03:21:44.13 0.6psi at evacEnd @03:21:44.15 Plunge.seek :pTop @03:21:45.03 delay 5 seconds @03:21:50.05 Air.in :glide @03:21:50.11 Air.seek :fullVent @03:21:52.75 delay 2 seconds @03:21:54.77 Plunge.seek :pBottom @03:21:55.62 Seawater evacuated, Pressure above/below filter released. @03:21:55.64 Plunge.seek :locked @03:21:56.95 Handoff.select :waste @03:21:57.04 Lysis1.in :slow @03:21:57.14 Lysis1.seek 950uL @03:22:11.21 delay 5 seconds @03:22:16.23 Handoff.select :blocked @03:22:16.37 Plunge.seek :puckTop @03:22:17.31 Lysis1.seek 350uL @03:22:45.31 delay 3 seconds @03:22:48.38 Air.in mix with 2.79934 parts Lysis1 @03:22:48.94 Air.seek :evac @03:22:48.99 Lysis1.seek 190uL @03:22:52.82 -> PhytoSampler.maxPostEvacPSI=6 6 @03:22:56.65 Air.in :fast @03:22:56.70 Air.seek :full @03:23:03.21 Lysis1.seek :empty @03:23:12.33 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @30.6psi @03:23:12.35 Plunge.seek :puckBottom @03:23:13.32 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @26.2psi @03:23:13.33 Polling Can every 2:00 @03:23:13.88 PuckHeater.configure :default #ambient 29.1C @03:23:13.94 PuckHeater.seek 81.3C @03:24:42.87 PuckHeater.hold 2:00,81.3C @03:25:17.12 -> exit @03:25:17.14 Session Ended @03:26:42.59 Polling Can every 2:00 @03:26:43.12 PuckHeater.configure :default #ambient 29.2C @03:26:43.16 PuckHeater.seek 80.2C @03:26:43.30 PuckHeater.hold 2:00,80.2C @03:28:43.05 Polling Can every 2:00 @03:28:43.58 PuckHeater.configure :default #ambient 29.2C @03:28:43.63 PuckHeater.hold 8:00,79.1C @03:36:41.90 Lysate cooked @36.8psi @03:36:41.92 Plunge.seek :puckTop @03:36:42.77 Air syringe pumping up to 5 strokes to reach 40psi @03:36:42.78 Air stroke #1 of 5 @03:36:42.84 Air.in :pressurize @03:36:42.88 Air.seek :pressurized @03:36:49.22 33.9psi after pressurize @03:36:49.24 Air.in :compress @03:36:49.28 Air.seek :evac @03:36:53.87 Reached 41.6 of 40.0psi after pumping 1661uL (0.94 strokes) @03:36:53.89 Air.in :default @03:36:53.93 Air.hold @03:36:53.98 delay 2 seconds @03:36:56.01 Air.in :fast @03:36:56.05 Air.seek :full @03:37:02.13 Plunge.seek :puckBottom @03:37:03.47 Lysis1.seek 138uL @03:37:10.00 delay 2 seconds @03:37:12.02 Plunge.seek :puckTop @03:37:12.87 Lysis1.seek :empty @03:37:19.40 Plunge.seek :puckBottom @03:37:20.43 Lysis1.in :extract @03:37:20.50 Lysis1.seek 675uL,nil @03:37:22.86 Air.in :pressurize @03:37:22.90 Air.seek :pressurized @03:37:29.25 9.9psi after pressurize @03:37:29.26 Air.in :compressLysate @03:37:29.30 Air.seek :evac @03:37:42.13 Reached 15.7 of 15.5psi after pumping 1588uL (0.89 strokes) @03:37:42.14 Air.in :default @03:37:42.19 Air.hold @03:37:43.51 Air.in :compressLysate @03:37:43.55 Air.seek :evac @03:37:47.31 Reached 15.4 of 15.5psi after pumping 1637uL (0.92 strokes) @03:37:47.33 Air.in :default @03:37:47.38 Air.hold @03:37:48.69 Air.in :compressLysate @03:37:48.73 Air.seek :evac @03:37:53.04 Reached 15.5 of 15.5psi after pumping 1696uL (0.96 strokes) @03:37:53.06 Air.in :default @03:37:53.10 Air.hold @03:37:54.34 Pulled 675uL lysate @14.9psi @03:37:54.47 Lysis1.seek 800uL,nil @03:37:55.70 Air.in :compressLysate @03:37:55.76 Air.seek :evac @03:38:01.50 15.5psi after compress @03:38:01.83 Reached 15.5 of 15.5psi after pumping 1775uL (1.00 strokes) @03:38:01.84 Air.in :default @03:38:01.88 Air.hold @03:38:02.39 Air.in :glide @03:38:02.43 Air.seek :fullVent @03:38:04.05 Handoff.select :waste @03:38:04.16 Recovered 800uL lysate @15.2psi @03:38:04.17 delay 3 seconds @03:38:07.23 After 3 delay Recovered 800uL lysate @0.7psi @03:38:07.39 Handoff.select :close @03:38:07.52
Gate.power :heat,:OFF @03:38:07.53 Gate.power :cartridge,:OFF @03:38:07.59 PinPort.in :unlocking @03:38:07.63 PinPort.seek :clear @03:38:08.43 Carousel.in :default @03:38:08.53 Carousel.home.seek 40 @03:38:08.80 Plunge.seek :locked @03:38:10.15 Archive.in :unlocking @03:38:10.24 Archive.seek :clear @03:38:10.32 Air.in :unlocking @03:38:10.40 Air.seek :clear @03:38:10.49 Lysis1.in :unlocking @03:38:10.59 Lysis1.seek :clear @03:38:10.66 Lysis2.in :unlocking @03:38:10.74 Lysis2.seek :clear @03:38:15.50
Gate.power :motor,:OFF @03:38:15.52 Gate.power :analytic1,:OFF @03:38:15.53 Gate.power :core,:OFF @03:38:15.54 Gate.power :main,:OFF @03:38:15.55 Safely stopped and ready to power off @03:38:16.46 Gate.power :sampler,:OFF,limit to 3500mA @03:38:16.49 Gate.power :skimmer,:OFF,limit to 3500mA @03:38:19.34 Gate.power :main,:ON,limit to 5000mA @03:38:19.36 Gate.power :analytic1,:ON,limit to 4000mA @03:38:19.41 Powering core bus @03:38:21.78 SPR Module 1, FW Rev. B, FPGA Rev. C @03:38:22.12
Plunge.configure :default @03:38:22.18 SamplePump.configure :default @03:38:22.25 Toroid.configure :default @03:38:22.31 Carousel.configure :default @03:38:22.37 Abs.configure :default @03:38:22.44 PhaseSeparator.configure :full @03:38:22.50 Archive.configure :default @03:38:22.56 Air.configure :default @03:38:22.60 Lysis1.configure :default @03:38:22.65 Lysis2.configure :default @03:38:22.71 PinPort.configure :default @03:38:22.75 All elf controllers running firmware version 4.94 @03:38:22.77 Gate.power :motor,:ON,limit to 5000mA @03:38:22.92 Plunge.home.jog 0 @03:38:23.48 PinPort.home.jog 0 @03:38:23.52 Lysis2.home.jog 0 @03:38:23.54 Lysis1.home.jog 0 @03:38:23.57 Archive.home.jog 0 @03:38:23.59 Carousel.home.jog 0 @03:38:23.61 Toroid.home.jog 0 @03:38:23.63 Air.home.jog 0 @03:38:24.26 Plunge.in :homing @03:38:24.42 Plunge.jog 1489 @03:38:24.46 Lysis2.in :homing @03:38:24.48 Lysis1.in :homing @03:38:24.50 Archive.in :homing @03:38:24.52 Toroid.in :homing @03:38:24.54 PinPort.in :homing @03:38:24.55 Air.in :homing @03:38:24.72 Lysis2.jog 1618 @03:38:24.81 Lysis1.jog 1593 @03:38:24.84 PinPort.jog 1223 @03:38:24.88 Archive.jog 1608 @03:38:24.91 Air.jog 1426 @03:38:24.93 Toroid.jog 1365 @03:38:25.81 Plunge.setRawPosition! 50 @03:38:25.85 Plunge.seek :home @03:38:26.11 Plunge.in :default @03:38:26.24 Lysis1.setRawPosition! 50 @03:38:26.26 Lysis2.setRawPosition! 50 @03:38:26.30 Air.setRawPosition! 0 @03:38:26.34 Lysis1.seek :home @03:38:26.40 Lysis2.seek :home @03:38:26.47 Air.seek :home @03:38:27.02 PinPort.setRawPosition! 3150 @03:38:27.12 Lysis2.in :default @03:38:27.14 Air.in :default @03:38:27.15 Archive.setRawPosition! 50 @03:38:27.17 Lysis1.in :default @03:38:27.29 PinPort.seek :home @03:38:27.45 Archive.seek :home @03:38:27.72 Archive.in :default @03:38:29.59 Toroid.setRawPosition! 0 @03:38:29.63 Toroid.seek :home @03:38:29.69 Toroid.in :default @03:38:31.69 PinPort.in :default @03:38:32.00 Toroid.seek :clear @03:38:32.12
PinPort.in :unlocking @03:38:32.18 PinPort.seek :clear @03:38:35.63 Air.seek :clear @03:38:35.70 Lysis1.seek :clear @03:38:36.40 Lysis2.seek :clear @03:38:37.17 Archive.seek :clear @03:38:37.93 Carousel.in :default @03:38:38.02 Carousel.home.seek 40 @03:38:38.25 Selecting Cartridge 50 @03:38:38.48 Carousel.seek 50 @03:38:40.82 Carousel.reconfigure :align @03:38:40.91 Lysis1.in :fast @03:38:40.95 Lysis1.seek :locked @03:38:43.53 Plunge.seek :locked @03:38:43.65 Air.in :fast @03:38:43.70 Air.seek :locked @03:38:44.75
Carousel.reconfigure :locked @03:38:44.87 Powering cartridge bus @03:38:47.48 Gate.setIR 1750,5000,0,6 @03:38:47.60 Gate.setIR 0,5000,0,6 @03:38:47.65 PuckHeater.configure :default #ambient 29.2C @03:38:47.70 Gate.power :heat,:ON,limit to 5000mA @03:38:47.83 Mounted lysis Cartridge board#J59986 @03:38:47.85 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:38:48.09 Preparing to filter microSPR_phyto cartridge in slot 50 @03:38:48.66 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @03:38:55.26 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @03:38:55.27 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @03:38:55.29 PhaseSeparator.in :filling @03:38:55.33 PhaseSeparator.hold @03:38:55.39 Gate.power :sampler,:ON @03:39:03.85 Head pressure reached 2.55psi in 9 seconds @03:39:03.87 Air.in :default @03:39:03.96 Priming sample loop w/200ml, bypass w/10ml @03:39:03.98 SamplePump.setPosition! 0ml @03:39:04.00 Air.seek :sealed @03:39:04.06 Bypass.open @03:39:06.33 Intake.open @03:39:07.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @03:39:10.50 Vacuum tare is 54% of full scale at 29C (drifted 2%) @03:39:10.60 Abs.in :default @03:39:10.64 Abs.hold @03:39:10.70 PhaseSeparator.in :full @03:39:10.74 PhaseSeparator.hold @03:39:10.91 Waiting for sampler to finish priming... @03:39:10.92 delayUntil "03:39:55.40" @03:39:55.45 SamplePump.in :start @03:39:55.50 SamplePump.seek 10ml,1:10 @03:39:56.14 SamplePump.configure :prime @03:40:08.72 SamplePump.stop @03:40:08.77 Intake.close @03:40:08.82 Bypass.close @03:40:10.44 Abs.stop @03:40:10.49 PhaseSeparator.stop @03:40:10.68 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only -1.2psi vacuum after 9.8ml @03:40:11.09 Retry #1 of 2 @03:40:11.11 Selecting Cartridge 42 @03:40:11.25 Gate.power :heat,:OFF @03:40:11.26 Gate.power :cartridge,:OFF @03:40:11.33 Carousel.in :default @03:40:11.44 Carousel.home.seek 50 @03:40:11.80 Air.in :unlocking @03:40:11.90 Air.seek :clear @03:40:13.45 Lysis1.in :unlocking @03:40:13.54 Lysis1.seek :clear @03:40:13.63 Plunge.in :unlocking @03:40:13.69 Plunge.seek :clear @03:40:13.74 Gate.power :sampler,:OFF @03:40:14.87 Carousel.seek 42 @03:40:16.82 Carousel.reconfigure :align @03:40:16.92 Lysis1.in :fast @03:40:16.96 Lysis1.seek :locked @03:40:19.55 Plunge.in :default @03:40:19.63 Plunge.seek :locked @03:40:19.74 Air.in :fast @03:40:19.79 Air.seek :locked @03:40:20.84 Carousel.reconfigure :locked @03:40:20.97 Powering cartridge bus @03:40:23.59 Gate.setIR 1750,5000,0,6 @03:40:23.71 Gate.setIR 0,5000,0,6 @03:40:23.77 PuckHeater.configure :default #ambient 29.2C @03:40:23.82 Gate.power :heat,:ON,limit to 5000mA @03:40:23.95 Mounted lysis Cartridge board#J60008 @03:40:23.98 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:40:24.17 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @03:40:30.77 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @03:40:30.78 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @03:40:30.81 PhaseSeparator.in :filling @03:40:31.18 PhaseSeparator.hold @03:40:31.31 Gate.power :sampler,:ON @03:40:39.73 Head pressure reached 2.49psi in 9 seconds @03:40:39.76 Air.in :default @03:40:39.87 Priming sample loop w/200ml, bypass w/10ml @03:40:39.89 Air.seek :sealed @03:40:39.91 SamplePump.setPosition! 0ml @03:40:39.99 Bypass.open @03:40:42.28 Intake.open @03:40:44.69 Abs.in :default @03:40:44.74 Abs.hold @03:40:44.80 PhaseSeparator.in :full @03:40:44.85 PhaseSeparator.hold @03:40:45.03 Waiting for sampler to finish priming... @03:40:45.04 delayUntil "03:41:31.32" @03:41:31.39 SamplePump.in :start @03:41:31.44 SamplePump.seek 10ml,1:10 @03:41:32.07 SamplePump.configure :prime @03:41:44.93 Abs.stop @03:41:44.98 PhaseSeparator.stop @03:41:45.02 SamplePump.setPosition! 0ml @03:41:45.06 Exhaust.open @03:41:47.22 Bypass.close @03:41:49.50 Abs.in :default @03:41:49.54 Abs.hold @03:41:49.60 PhaseSeparator.hold @03:41:49.86 SamplePump.in :start @03:41:49.93 SamplePump.seek 200ml,13:50 @03:41:50.55 SamplePump.configure :prime @03:42:10.24 SamplePump.stop @03:42:10.29 Intake.close @03:42:10.32 Exhaust.close @03:42:11.99 Abs.stop @03:42:12.04 PhaseSeparator.stop @03:42:12.23 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 4.2psi vacuum after 15.2ml @03:42:12.24 Retry #2 of 2 @03:42:12.27 Selecting Cartridge 36 @03:42:12.41 Gate.power :heat,:OFF @03:42:12.42 Gate.power :cartridge,:OFF @03:42:12.48 Carousel.in :default @03:42:12.59 Carousel.home.seek 42 @03:42:13.38 Air.in :unlocking @03:42:13.47 Air.seek :clear @03:42:15.03 Lysis1.in :unlocking @03:42:15.15 Lysis1.seek :clear @03:42:15.22 Plunge.in :unlocking @03:42:15.29 Plunge.seek :clear @03:42:15.34 Gate.power :sampler,:OFF @03:42:16.52 Carousel.seek 36 @03:42:18.07 Carousel.reconfigure :align @03:42:18.18 Lysis1.in :fast @03:42:18.23 Lysis1.seek :locked @03:42:20.82 Plunge.in :default @03:42:20.89 Plunge.seek :locked @03:42:20.99 Air.in :fast @03:42:21.04 Air.seek :locked @03:42:22.09 Carousel.reconfigure :locked @03:42:22.22 Powering cartridge bus @03:42:24.85 Gate.setIR 1750,5000,0,6 @03:42:24.97 Gate.setIR 0,5000,0,6 @03:42:25.02 PuckHeater.configure :default #ambient 29.2C @03:42:25.08 Gate.power :heat,:ON,limit to 5000mA @03:42:25.20 Mounted lysis Cartridge board#J59943 @03:42:25.23 Using default cartridge heat loss curve for HeatingFunctionInCarousel @03:42:25.43 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @03:42:32.02 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @03:42:32.04 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @03:42:32.07 PhaseSeparator.in :filling @03:42:32.11 PhaseSeparator.hold @03:42:32.17 Gate.power :sampler,:ON @03:42:40.61 Head pressure reached 2.58psi in 9 seconds @03:42:40.63 Air.in :default @03:42:40.73 Priming sample loop w/200ml, bypass w/10ml @03:42:40.76 Air.seek :sealed @03:42:40.78 SamplePump.setPosition! 0ml @03:42:41.22 Bypass.open @03:42:43.60 Intake.open @03:42:46.00 Abs.in :default @03:42:46.04 Abs.hold @03:42:46.10 PhaseSeparator.in :full @03:42:46.15 PhaseSeparator.hold @03:42:46.33 Waiting for sampler to finish priming... @03:42:46.34 delayUntil "03:43:32.19" @03:43:32.25 SamplePump.in :start @03:43:32.30 SamplePump.seek 10ml,1:10 @03:43:32.94 SamplePump.configure :prime @03:43:45.79 Abs.stop @03:43:45.83 PhaseSeparator.stop @03:43:45.87 SamplePump.setPosition! 0ml @03:43:45.92 Exhaust.open @03:43:48.06 Bypass.close @03:43:50.36 Abs.in :default @03:43:50.40 Abs.hold @03:43:50.47 PhaseSeparator.hold @03:43:50.73 SamplePump.in :start @03:43:50.80 SamplePump.seek 200ml,13:50 @03:43:51.42 SamplePump.configure :prime @03:44:17.69 SamplePump.stop @03:44:17.74 Intake.close @03:44:17.77 Exhaust.close @03:44:19.50 Abs.stop @03:44:19.54 PhaseSeparator.stop @03:44:19.73 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 4.0psi vacuum after 20.2ml @03:44:19.74 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 20.21ml" @03:44:22.65 Gate.power :sampler,:OFF @04:10:02.91

Session Begun @04:12:01.84 -> slots {2 => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, [32,24,22,20,18,16] => { Type: :archiveHiBiomass_bac, State: :dry}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [50,36] => { Type: :microSPR_phyto, State: :dry, rank: 1}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000.0, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @04:13:19.41 -> MainThrad.exit @04:13:19.43 NameError in p -- uninitialized constant MainThrad @04:13:30.54 -> MainThread.exit # @04:13:30.69 ThreadError in p -- killed thread @04:13:30.75 Session Ended @04:14:01.25PDT20-Jul-26

Environmental Sample Processor built 17-Jul-26 (24b6d875) @04:14:02.57 >BREAK< ESP3 Gateway v1.44 for core bus @04:14:02.59 >BREAK< ESP3 Gateway v1.44 for cartridge bus @04:14:05.32 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @04:14:05.84 Sea pressure (Abs) tare is 6% of full scale @04:14:09.85 Polling Can every 2:00 @04:14:09.88 Loading /home/esp/esp2/bin/fireAll mission @04:14:10.09 Cartridge group: dry Spares: {spare: 3} @04:14:10.22 Gate.power :sampler,:OFF,limit to 3500mA @04:14:10.23 Gate.power :skimmer,:OFF,limit to 3500mA @04:14:10.24 Gate.power :analytic1,:ON,limit to 4000mA @04:14:10.79 SPR Module 1, FW Rev. B, FPGA Rev. C @04:14:10.89
Plunge.configure :default @04:14:10.93 Plunge.hold @04:14:10.98 Plunge.configure :default @04:14:11.09 SamplePump.configure :default @04:14:11.56 Toroid.configure :default @04:14:11.60 Toroid.hold @04:14:11.65 Toroid.configure :default @04:14:11.84 Carousel.configure :default @04:14:11.90 Carousel.hold @04:14:12.01 Carousel.configure :default @04:14:12.20 Abs.configure :default @04:14:12.41 PhaseSeparator.configure :default @04:14:12.60 Archive.configure :default @04:14:12.70 Archive.hold @04:14:12.80 Archive.configure :default @04:14:13.00 Air.configure :default @04:14:13.08 Air.hold @04:14:13.56

Session Begun @04:14:13.64

Air.configure :default @04:14:13.77 Lysis1.configure :default @04:14:13.83 Lysis1.hold @04:14:13.90 Lysis1.configure :default @04:14:14.01 Lysis2.configure :default @04:14:14.07 Lysis2.hold @04:14:14.14 Lysis2.configure :default @04:14:14.28 PinPort.configure :default @04:14:14.33 PinPort.hold @04:14:14.41 PinPort.configure :default @04:14:14.46 All elf controllers running firmware version 4.94 @04:14:14.99 Toroid.seek :clear @04:14:15.14
Air.seek :clear @04:14:19.86 Lysis1.seek :clear @04:14:23.91 Lysis2.seek :clear @04:14:24.00 Archive.seek :clear @04:14:24.09 Carousel.in :default @04:14:24.20 Carousel.home.seek 36 @04:14:24.50 Selecting Cartridge 32 @04:14:24.59 Gate.power :heat,:OFF @04:14:24.60 Gate.power :cartridge,:OFF @04:14:24.70 Carousel.in :default @04:14:24.80 Carousel.home.seek 36 @04:14:25.32 Plunge.in :unlocking @04:14:25.75 Plunge.seek :clear @04:14:26.75
Carousel.seek 32 @04:14:27.97 Carousel.reconfigure :align @04:14:28.09 Lysis1.in :fast @04:14:28.15 Lysis1.seek :locked @04:14:28.23 Slide::Warning in core -- Storage Carousel (SC) reseek at 32 + 4 counts @04:14:30.73 Plunge.in :default @04:14:30.82 Plunge.seek :locked @04:14:30.92 Air.in :fast @04:14:31.00 Air.seek :locked @04:14:32.06
Carousel.reconfigure :locked @04:14:32.20 Powering cartridge bus @04:14:35.82 Gate.setIR 1750,5000,0,6 @04:14:35.95 Gate.setIR 0,5000,0,6 @04:14:36.04 Gate.power :cartridge,:OFF @04:14:36.07 Mounted archive Cartridge @04:14:36.34 Preparing to filter archiveHiBiomass_bac cartridge in slot 32 @04:14:36.54 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @04:14:43.15 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @04:14:43.16 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @04:14:43.20 PhaseSeparator.in :filling @04:14:43.24 PhaseSeparator.hold @04:14:43.32 Gate.power :sampler,:ON @04:14:49.37

-> PhytoSampler.maxPostEvacPSI=6 6 @04:14:51.78 Head pressure reached 2.37psi in 9 seconds @04:14:51.86 Priming sample loop w/200ml, bypass w/10ml @04:14:51.88 SamplePump.setPosition! 0ml @04:14:51.93 Exhaust.close @04:14:52.03 Bypass.open @04:14:54.26 Intake.open @04:14:55.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @04:14:58.50 Vacuum tare is 54% of full scale at 29C (drifted 2%) @04:14:58.69 Abs.in :default @04:14:58.75 Abs.hold @04:14:58.83 PhaseSeparator.in :full @04:14:58.88 PhaseSeparator.hold @04:14:59.07 Waiting for sampler to finish priming... @04:14:59.08 delayUntil "04:15:43.33" @04:15:43.38 SamplePump.in :start @04:15:43.45 SamplePump.seek 10ml,1:10 @04:15:44.08 SamplePump.configure :prime @04:15:56.96 Abs.stop @04:15:57.00 PhaseSeparator.stop @04:15:57.07 SamplePump.setPosition! 0ml @04:15:57.12 Exhaust.open @04:15:59.29 Bypass.close @04:16:01.57 Abs.in :default @04:16:01.64 Abs.hold @04:16:01.71 PhaseSeparator.hold @04:16:01.99 SamplePump.in :start @04:16:02.06 SamplePump.seek 200ml,13:50 @04:16:02.68 SamplePump.configure :prime @04:16:07.32 SamplePump.stop @04:16:07.39 Intake.close @04:16:07.43 Exhaust.close @04:16:09.05 Abs.stop @04:16:09.11 PhaseSeparator.stop @04:16:09.33 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 3.2psi vacuum after 3.7ml @04:16:09.35 Retry #1 of 2 @04:16:09.39 Selecting Cartridge 24 @04:16:09.60 Carousel.in :default @04:16:09.73 Carousel.home.seek 32 @04:16:10.14 Air.in :unlocking @04:16:10.24 Air.seek :clear @04:16:11.29 Lysis1.in :unlocking @04:16:11.41 Lysis1.seek :clear @04:16:11.52 Plunge.in :unlocking @04:16:11.58 Plunge.seek :clear @04:16:12.25 Gate.power :sampler,:OFF @04:16:13.00 Carousel.seek 24 @04:16:14.97 Carousel.reconfigure :align @04:16:15.10 Lysis1.in :fast @04:16:15.16 Lysis1.seek :locked @04:16:17.78 Plunge.in :default @04:16:17.87 Plunge.seek :locked @04:16:18.00 Air.in :fast @04:16:18.05 Air.seek :locked @04:16:19.12 Carousel.reconfigure :locked @04:16:19.26 Powering cartridge bus @04:16:21.88 Gate.setIR 1750,5000,0,6 @04:16:22.01 Gate.setIR 0,5000,0,6 @04:16:22.10 Gate.power :cartridge,:OFF @04:16:22.15 Mounted archive Cartridge @04:16:22.36 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @04:16:28.97 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @04:16:28.98 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @04:16:29.03 PhaseSeparator.in :filling @04:16:29.10 PhaseSeparator.hold @04:16:29.17 Gate.power :sampler,:ON @04:16:37.57 Head pressure reached 2.51psi in 9 seconds @04:16:37.65 Priming sample loop w/200ml, bypass w/10ml @04:16:37.67 SamplePump.setPosition! 0ml @04:16:37.74 Bypass.open @04:16:40.06 Intake.open @04:16:42.39 Abs.in :default @04:16:42.45 Abs.hold @04:16:42.55 PhaseSeparator.in :full @04:16:42.61 PhaseSeparator.hold @04:16:42.81 Waiting for sampler to finish priming... @04:16:42.83 delayUntil "04:17:29.19" @04:17:04.51

-> Hesd.psi @04:17:04.53 NameError in p -- uninitialized constant Hesd @04:17:15.40 -> Head.psi 2.43378898563433 @04:17:17.57 -> Head.psi 2.46906640244306 @04:17:18.80 -> Head.psi 2.39727685923729 @04:17:29.25 SamplePump.in :start @04:17:29.33 SamplePump.seek 10ml,1:10 @04:17:29.94 SamplePump.configure :prime @04:17:31.21

-> Head.psi 2.43943337232372 @04:17:43.19 Abs.stop @04:17:43.33 PhaseSeparator.stop @04:17:43.39 SamplePump.setPosition! 0ml @04:17:43.45 Exhaust.open @04:17:45.64 Bypass.close @04:17:47.94 Abs.in :default @04:17:48.01 Abs.hold @04:17:48.09 PhaseSeparator.hold @04:17:48.39 SamplePump.in :start @04:17:48.47 SamplePump.seek 200ml,13:50 @04:17:49.08 SamplePump.configure :prime @04:17:52.78 SamplePump.stop @04:17:52.86 Intake.close @04:17:52.94 Exhaust.close @04:17:53.42

-> loop {puts Sampler.psi; Delay.sleep 1} @04:17:54.52 Abs.stop @04:17:54.58 PhaseSeparator.stop @04:17:54.85 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 1.4psi vacuum after 3.0ml @04:17:54.87 Retry #2 of 2 @04:17:54.91 Selecting Cartridge 22 @04:17:55.15 Carousel.in :default @04:17:55.29 Carousel.home.seek 24 @04:17:55.68 Air.in :unlocking @04:17:55.81 Air.seek :clear @04:17:56.87 Lysis1.in :unlocking @04:17:56.98 Lysis1.seek :clear @04:17:57.09 Plunge.in :unlocking @04:17:57.17 Plunge.seek :clear @04:17:57.72 Gate.power :sampler,:OFF @04:17:58.36 Carousel.seek 22 @04:17:59.12 Carousel.reconfigure :align @04:17:59.24 Lysis1.in :fast @04:17:59.31 Lysis1.seek :locked @04:17:59.40 Slide::Warning in core -- Storage Carousel (SC) reseek at 22 + 5 counts @04:18:00.13 UserAbort in p -- User Abort!! @04:18:01.92 Plunge.in :default @04:18:02.02 Plunge.seek :locked @04:18:02.14 Air.in :fast @04:18:02.19 Air.seek :locked @04:18:03.27 Carousel.reconfigure :locked @04:18:03.49 Powering cartridge bus @04:18:06.11 Gate.setIR 1750,5000,0,6 @04:18:06.24 Gate.setIR 0,5000,0,6 @04:18:06.33 Gate.power :cartridge,:OFF @04:18:06.37 Mounted archive Cartridge @04:18:06.59 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @04:18:13.20 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @04:18:13.21 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @04:18:13.26 PhaseSeparator.in :filling @04:18:13.32 PhaseSeparator.hold @04:18:13.40 Gate.power :sampler,:ON @04:18:21.88 Head pressure reached 2.59psi in 9 seconds @04:18:21.97 Priming sample loop w/200ml, bypass w/10ml @04:18:21.98 SamplePump.setPosition! 0ml @04:18:22.06 Bypass.open @04:18:24.38 Intake.open @04:18:26.74 Abs.in :default @04:18:26.80 Abs.hold @04:18:26.90 PhaseSeparator.in :full @04:18:26.96 PhaseSeparator.hold @04:18:27.17 Waiting for sampler to finish priming... @04:18:27.19 delayUntil "04:19:13.42" @04:18:27.81

-> loop {puts Sampler.psi; Delay.sleep 1} @04:19:13.61 SamplePump.in :start @04:19:13.69 SamplePump.seek 10ml,1:10 @04:19:14.25 SamplePump.configure :prime @04:19:27.20 Abs.stop @04:19:27.25 PhaseSeparator.stop @04:19:27.32 SamplePump.setPosition! 0ml @04:19:27.38 Exhaust.open @04:19:29.62 Bypass.close @04:19:31.95 Abs.in :default @04:19:32.02 Abs.hold @04:19:32.10 PhaseSeparator.hold @04:19:32.38 SamplePump.in :start @04:19:32.46 SamplePump.seek 200ml,13:50 @04:19:33.07 SamplePump.configure :prime @04:20:59.35 UserAbort in p -- User Abort!! @04:21:05.00

-> SP Sample Pump (SP) at 70.33ml @04:21:26.69 -> SP Sample Pump (SP) at 86.87ml @04:22:00.79 SamplePump.stop @04:22:00.85 Intake.close @04:22:00.90 Exhaust.close @04:22:02.54 Abs.stop @04:22:02.60 PhaseSeparator.stop @04:22:02.82 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only 1.3psi vacuum after 112.8ml @04:22:02.84 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 112.77ml" @04:22:05.74 Gate.power :sampler,:OFF @04:23:06.57

-> PhytoSampler.primeVac 5..50 @04:30:04.01 -> can! @04:30:04.04 Polling Can every 2:00 Can@04:30:04, 29.0C, 45% humidity, 14.7psia, 14.961V, 0.498A, 8.592Ah, 7.46W @04:31:34.71 -> Power.sampler :on @04:31:34.73 Gate.power :sampler,:ON true @04:31:38.17 -> Head.psi 0.273796851206761 @04:31:40.26 -> Head.psi 3.23692347605609 @04:31:41.13 -> Head.psi 2.56524146001786 @04:31:41.99 -> Head.psi 2.51373643147711 @04:31:43.32 -> Head.psi 2.53772507490705 @04:31:44.66 -> Head.psi 2.49309914264401 @04:31:55.73 -> Sampler.refill @04:31:55.75 Bypass.open @04:31:57.27 Intake.open @04:31:58.87 SamplePump.setPosition! 0ml @04:31:58.97 SamplePump.in :default @04:31:59.03 SamplePump.seek 30ml @04:32:38.95 Bypass.close @04:32:40.42 Exhaust.open @04:32:41.96 SamplePump.seek 55ml @04:33:15.33 delay 1 second @04:33:16.36 Intake.close @04:33:16.38 Exhaust.close Sample Pump (SP) at 55ml @04:33:33.10

-> Intake.open; Exhaust.open @04:33:33.12 Intake.open @04:33:34.77 Exhaust.open Exhaust is opened @04:33:59.35 -> Thread[:FILTERING].recover @04:33:59.39 Recovering Thread after Serious Leak or Stall -- 0.76ml/s made only 1.3psi vacuum after 112.8ml at 04:22:02 # @04:33:59.46 Selecting Cartridge 20 @04:33:59.68 Carousel.in :default @04:33:59.81 Carousel.home.seek 22 @04:34:00.18 Air.in :unlocking @04:34:00.31 Air.seek :clear @04:34:01.37 Lysis1.in :unlocking @04:34:01.50 Lysis1.seek :clear @04:34:01.58 Plunge.in :unlocking @04:34:01.67 Plunge.seek :clear @04:34:02.88 Carousel.seek 20 @04:34:03.61 Carousel.reconfigure :align @04:34:03.74 Lysis1.in :fast @04:34:03.80 Lysis1.seek :locked @04:34:05.17 Slide::Warning in core -- Storage Carousel (SC) reseek at 20 - 5 counts (sensed 20 - 4 counts) @04:34:06.40 Plunge.in :default @04:34:06.50 Plunge.seek :locked @04:34:06.62 Air.in :fast @04:34:06.69 Air.seek :locked @04:34:07.75 Carousel.reconfigure :locked @04:34:07.89 Powering cartridge bus @04:34:10.52 Gate.setIR 1750,5000,0,6 @04:34:10.64 Gate.setIR 0,5000,0,6 @04:34:10.74 Gate.power :cartridge,:OFF @04:34:10.77 Mounted archive Cartridge @04:34:10.99 Gate.power :sampler,:OFF @04:34:11.37 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @04:34:17.97 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @04:34:17.99 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @04:34:18.04 PhaseSeparator.in :filling @04:34:18.09 PhaseSeparator.hold @04:34:18.18 Gate.power :sampler,:ON @04:34:27.10 Head pressure reached 2.33psi in 10 seconds @04:34:27.19 Priming sample loop w/200ml, bypass w/10ml @04:34:27.21 SamplePump.setPosition! 0ml @04:34:27.28 Exhaust.close @04:34:28.75 Bypass.open @04:34:31.12 Abs.in :default @04:34:31.19 Abs.hold @04:34:31.27 PhaseSeparator.in :full @04:34:31.34 PhaseSeparator.hold @04:34:31.54 Waiting for sampler to finish priming... @04:34:31.56 delayUntil "04:35:18.20" @04:35:18.27 SamplePump.in :start @04:35:18.35 SamplePump.seek 10ml,1:10 @04:35:18.95 SamplePump.configure :prime @04:35:31.87 Abs.stop @04:35:31.93 PhaseSeparator.stop @04:35:31.99 SamplePump.setPosition! 0ml @04:35:32.05 Exhaust.open @04:35:34.22 Bypass.close @04:35:36.51 Abs.in :default @04:35:36.57 Abs.hold @04:35:36.66 PhaseSeparator.hold @04:35:36.95 SamplePump.in :start @04:35:37.02 SamplePump.seek 200ml,13:50 @04:35:37.64 SamplePump.configure :prime @04:36:41.63

-> SP Sample Pump (SP) at 49.05ml @04:36:54.34 -> loop {puts Sampler.psi; Delay.sleep 1} @04:39:13.36 UserAbort in p -- User Abort!! @04:39:15.88 -> SP Sample Pump (SP) at 166.65ml @04:39:25.23 -> SP Sample Pump (SP) at 173.78ml @04:39:27.89 -> SP Sample Pump (SP) at 175.82ml @04:39:32.47 -> loop {puts Sampler.psi; Delay.sleep 1} @04:39:53.26 UserAbort in p -- User Abort!! @04:39:59.76 Abs.stop @04:39:59.82 PhaseSeparator.stop @04:39:59.90 Bypass.open @04:40:01.37 Intake.close @04:40:03.07 Toroid.in :hold @04:40:03.14 Toroid.seek :crack @04:40:05.63 Toroid.seek :cartridge @04:40:07.76 Toroid.in :default @04:40:07.82 Toroid.seek :clear @04:40:10.46

-> PhytoSampler.maxPostEvacPSI 6 @04:40:11.76 Pressurizing cartridge to 26psi above 13.9psia @04:40:11.77 SamplePump.setPosition! 0ml @04:40:11.85 Intake.open @04:40:13.43 Bypass.close @04:40:14.94 Exhaust.close @04:40:16.94 Abs.in :default @04:40:17.09 Abs.hold @04:40:17.18 PhaseSeparator.hold @04:40:17.40 SamplePump.in :charge @04:40:17.46 SamplePump.seek 2.3ml,40 seconds @04:40:25.14 Abs.stop @04:40:25.20 PhaseSeparator.stop @04:40:25.26 Intake.close @04:40:26.97 Pressurized cartridge with 0.240ml to 26.3psi @04:40:26.99 Isolated @38.1psia (24.2psi) @04:40:27.02 Verifying cartridge holds at least 0.65..0.85 of 38.1psia @04:40:27.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @04:40:31.70 Sampling 1000.0ml @04:40:31.71 SamplePump.setPosition! 0ml @04:40:31.78 Exhaust.open @04:40:33.27 Intake.open @04:40:35.01 Abs.in :default @04:40:35.07 Abs.hold @04:40:35.16 PhaseSeparator.hold @04:40:35.45 SamplePump.in :start @04:40:35.53 SamplePump.seek 1000ml,1:07:10 @04:40:36.14 SamplePump.configure :sample @04:40:38.52 Cartridge 20 filtering 2/1000ml at 0.76ml/s with 7.4psi vacuum 6.9m deep (25.0psia) @04:40:53.18

-> exit @04:40:53.20 Session Ended @04:41:38.57 Cartridge 20 filtering 48/1000ml at 0.76ml/s with 11.4psi vacuum @04:42:38.57 Cartridge 20 filtering 93/1000ml at 0.76ml/s with 11.2psi vacuum @04:43:38.57 Cartridge 20 filtering 139/1000ml at 0.76ml/s with 15.5psi vacuum @04:44:38.57 Cartridge 20 filtering 185/1000ml at 0.76ml/s with 13.7psi vacuum @04:45:38.57 Cartridge 20 filtering 231/1000ml at 0.76ml/s with 8.8psi vacuum 7.3m deep (25.5psia) @04:46:38.57 Cartridge 20 filtering 276/1000ml at 0.76ml/s with 10.4psi vacuum @04:47:38.58 Cartridge 20 filtering 322/1000ml at 0.76ml/s with 12.8psi vacuum @04:47:58.65 Session Begun @04:48:00.71 -> slots {2 => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, [18,16] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :archiveHiBiomass_bac, State: :filtering, TV: :open}, [32,24,22] => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [50,36] => { Type: :microSPR_phyto, State: :dry, rank: 1}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @04:48:29.02 -> slots [50,36]=>nil {[50,36] => nil} @04:48:38.57 Cartridge 20 filtering 368/1000ml at 0.76ml/s with 19.6psi vacuum @04:48:41.87 -> slots [50,36]=:microSPR_phyto @04:48:41.90 SyntaxError in preston -- compile error preston:3: syntax error, unexpected '=', expecting $end slots [50,36]=:microSPR_phyto ^ @04:48:51.02 -> slots [50,36]=>:microSPR_phyto {[50,36] => { Type: :microSPR_phyto, State: :dry}} @04:48:53.50 -> exit @04:48:53.52 Session Ended @04:49:38.64 Cartridge 20 filtering 414/1000ml at 0.76ml/s with 16.7psi vacuum @04:50:38.57 Cartridge 20 filtering 460/1000ml at 0.76ml/s with 11.8psi vacuum @04:51:38.57 Cartridge 20 filtering 505/1000ml at 0.76ml/s with 16.5psi vacuum @04:52:38.56 Cartridge 20 filtering 551/1000ml at 0.76ml/s with 20.4psi vacuum @04:53:38.57 Cartridge 20 filtering 597/1000ml at 0.76ml/s with 15.3psi vacuum @04:54:38.57 Cartridge 20 filtering 642/1000ml at 0.76ml/s with 12.0psi vacuum 5.8m deep (23.3psia) @04:55:38.57 Cartridge 20 filtering 688/1000ml at 0.76ml/s with 12.6psi vacuum @04:56:38.56 Cartridge 20 filtering 734/1000ml at 0.76ml/s with 17.6psi vacuum @04:57:38.57 Cartridge 20 filtering 780/1000ml at 0.76ml/s with 20.4psi vacuum @04:58:38.57 Cartridge 20 filtering 826/1000ml at 0.76ml/s with 11.7psi vacuum 6.2m deep (23.9psia) @04:59:38.57 Cartridge 20 filtering 871/1000ml at 0.76ml/s with 15.3psi vacuum @05:00:38.57 Cartridge 20 filtering 917/1000ml at 0.76ml/s with 12.1psi vacuum @05:01:38.57 Cartridge 20 filtering 963/1000ml at 0.76ml/s with 15.3psi vacuum @05:02:26.98 Abs.stop @05:02:27.03 PhaseSeparator.stop @05:02:27.08 Intake.close @05:02:27.13 Exhaust.close @05:02:27.31 Toroid.in :hold @05:02:27.36 Toroid.seek :bypass @05:02:30.95 Toroid.in :default @05:02:30.99 Toroid.seek :clear @05:02:34.55 Sampled 1000.0ml @05:02:35.02 Plunge.seek :pTop @05:02:35.89 Sample loop closed. Ready to Deliver Reagents @05:02:35.90 Air.in :default @05:02:35.94 Air.seek :sealed @05:02:37.65 Gate.power :sampler,:OFF @05:02:38.67 Air.in :slow @05:02:38.71 Air.seek :arEvac @05:02:45.83 Slide::Error in PROCESSING -- Air Syringe (AirS) positionErr at 1442uL (sensed 1467uL) @05:02:45.85 Retry #1 of 2 @05:02:45.86 Air.in :default @05:02:45.90 Air.hold @05:02:45.97 Air.in :slow @05:02:46.02 Air.home.seek :arEvac @05:02:47.58 Slide::Error in PROCESSING -- Air Syringe (AirS) positionErr at 1437uL (sensed 1462uL) @05:02:47.59 Retry #2 of 2 @05:02:47.60 Air.in :default @05:02:47.64 Air.hold @05:02:47.72 Air.in :slow @05:02:47.76 Air.home.seek :arEvac @05:02:49.63 Slide::Error in PROCESSING -- IGNORED Air Syringe (AirS) positionErr at 1425uL (sensed 1449uL) @05:02:49.64 Air.in :default @05:02:49.68 Air.hold @05:02:49.75 Air.in :default @05:02:49.80 Air.home.jog 0 @05:02:50.31 ArchiveError in PROCESSING -- 1424ul RNAlater undelivered @05:02:50.33 Waiting to Resume from Checkpoint "IGNORED Air Syringe (AirS) positionErr at 1425uL (sensed 1449uL)" @07:16:20.34 Session Begun @07:16:47.31 -> slot {[50,36,2] => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, [18,16] => { Type: :archiveHiBiomass_bac, State: :dry}, 20 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, [32,24,22] => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @07:16:52.55 -> SC Storage Carousel (SC) at 20 @07:17:05.80 -> PV Plunge Valve (PV) at puckTop @07:17:28.75 -> Cart.dry [50,36,18,16,2,42,32,24,22] @07:17:48.51 -> Air Air Syringe (AirS) at 1450uL @07:17:54.67 -> Air.push 100 @07:17:54.76 Air.seek 1350uL @07:17:55.56 Slide::Error in preston -- Air Syringe (AirS) positionErr at 1393uL (sensed 1423uL) @07:18:15.04 -> #check check valves @07:18:20.83 -> MainThread.exit # @07:18:20.98 ThreadError in preston -- killed thread @07:18:21.03 Session Ended @07:18:37.01PDT20-Jul-26

Environmental Sample Processor built 17-Jul-26 (24b6d875) @07:18:38.39 >BREAK< ESP3 Gateway v1.44 for core bus @07:18:38.41 >BREAK< ESP3 Gateway v1.44 for cartridge bus @07:18:41.22 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @07:18:41.75 Sea pressure (Abs) tare is 6% of full scale @07:18:45.92 Polling Can every 2:00 @07:18:45.95 Loading /home/esp/esp2/bin/fireAll mission @07:18:46.16 Cartridge group: dry Spares: {spare: 3} @07:18:46.30 Gate.power :sampler,:OFF,limit to 3500mA @07:18:46.32 Gate.power :skimmer,:OFF,limit to 3500mA @07:18:46.33 Gate.power :analytic1,:ON,limit to 4000mA @07:18:46.88 SPR Module 1, FW Rev. B, FPGA Rev. C @07:18:46.98
Plunge.configure :default @07:18:47.03 Plunge.hold @07:18:47.08 Plunge.configure :default @07:18:47.19 SamplePump.configure :default @07:18:47.67 Toroid.configure :default @07:18:47.71 Toroid.hold @07:18:47.77 Toroid.configure :default @07:18:47.90 Carousel.configure :default @07:18:47.94 Carousel.hold @07:18:47.99 Carousel.configure :default @07:18:48.10 Abs.configure :default @07:18:48.21 PhaseSeparator.configure :default @07:18:48.32 Archive.configure :default @07:18:48.36 Archive.hold @07:18:48.41 Archive.configure :default @07:18:48.52 Air.configure :default @07:18:48.61 Lysis1.configure :default @07:18:48.65 Lysis1.hold @07:18:48.71 Lysis1.configure :default @07:18:48.79 Lysis2.configure :default @07:18:48.83 Lysis2.hold @07:18:48.89 Lysis2.configure :default @07:18:49.00 PinPort.configure :default @07:18:49.04 PinPort.hold @07:18:49.09 PinPort.configure :default @07:18:49.13 All elf controllers running firmware version 4.94 @07:18:49.69 Air.in :default @07:18:49.74 Air.home.jog 0 @07:18:49.88 Air.in :homing @07:18:49.93 Air.jog 3324 @07:18:51.99 Air.setRawPosition! 0 @07:18:52.03 Air.seek :home @07:18:52.10 Air.in :default @07:18:52.41 Toroid.seek :clear @07:18:52.55
Air.seek :clear @07:18:52.62 Lysis1.seek :clear @07:18:56.64 Lysis2.seek :clear @07:18:56.71 Archive.seek :clear @07:18:56.77 Carousel.in :default @07:18:56.86 Carousel.home.seek 20 @07:18:57.16 Selecting Cartridge 50 @07:18:57.29 Carousel.in :default @07:18:57.39 Carousel.home.seek 20 @07:18:58.29 Plunge.in :unlocking @07:18:58.35 Plunge.seek :clear @07:18:59.76
Carousel.seek 50 @07:19:06.16 Carousel.reconfigure :align @07:19:06.26 Lysis1.in :fast @07:19:06.30 Lysis1.seek :locked @07:19:06.40 Slide::Warning in core -- Storage Carousel (SC) reseek at 50 + 4 counts @07:19:08.88 Plunge.in :default @07:19:08.93 Plunge.seek :locked @07:19:09.06 Air.in :fast @07:19:09.10 Air.seek :locked @07:19:10.16
Carousel.reconfigure :locked @07:19:10.28 Powering cartridge bus @07:19:12.90 Gate.setIR 1750,5000,0,6 @07:19:13.01 Gate.setIR 0,5000,0,6 @07:19:13.07 PuckHeater.configure :default @07:19:13.11 Gate.power :heat,:ON,limit to 5000mA @07:19:15.26 Mounted lysis Cartridge board#J59986 @07:19:15.29 Using default cartridge heat loss curve for HeatingFunctionInCarousel @07:19:15.52 Preparing to filter microSPR_phyto cartridge in slot 50 @07:19:15.70 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:19:22.29 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @07:19:22.31 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @07:19:22.33 PhaseSeparator.in :filling @07:19:22.37 PhaseSeparator.hold @07:19:22.43 Gate.power :sampler,:ON @07:19:30.92 Head pressure reached 2.52psi in 9 seconds @07:19:30.95 Air.in :default @07:19:31.03 Priming sample loop w/200ml, bypass w/10ml @07:19:31.06 SamplePump.setPosition! 0ml @07:19:31.10 Air.seek :sealed @07:19:31.13 Exhaust.close @07:19:31.24 Bypass.open @07:19:33.47 Intake.open @07:19:35.10 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:19:37.75 Vacuum tare is 54% of full scale at 29C (drifted 2%) @07:19:37.87 Abs.in :default @07:19:37.91 Abs.hold @07:19:37.97 PhaseSeparator.in :full @07:19:38.01 PhaseSeparator.hold @07:19:38.18 Waiting for sampler to finish priming... @07:19:38.20 delayUntil "07:20:22.44" @07:20:22.50 SamplePump.in :start @07:20:22.55 SamplePump.seek 10ml,1:10 @07:20:23.19 SamplePump.configure :prime @07:20:36.02 Abs.stop @07:20:36.06 PhaseSeparator.stop @07:20:36.10 SamplePump.setPosition! 0ml @07:20:36.14 Exhaust.open @07:20:38.34 Bypass.close @07:20:40.64 Abs.in :default @07:20:40.68 Abs.hold @07:20:40.74 PhaseSeparator.hold @07:20:40.99 SamplePump.in :start @07:20:41.04 SamplePump.seek 200ml,13:50 @07:20:41.67 SamplePump.configure :prime @07:20:56.09 Session Begun @07:21:04.23 -> PhytoSampler.maxPostEvacPSI=6 6 @07:21:16.66 -> exit @07:21:16.68 Session Ended @07:25:03.54 Abs.stop @07:25:03.58 PhaseSeparator.stop @07:25:03.64 Bypass.open @07:25:05.14 Intake.close @07:25:06.88 Toroid.in :hold @07:25:06.92 Toroid.seek :crack @07:25:09.37 Toroid.seek :cartridge @07:25:11.47 Toroid.in :default @07:25:11.51 Toroid.seek :clear @07:25:15.41 Pressurizing cartridge to 14psi above 14.0psia @07:25:15.43 SamplePump.setPosition! 0ml @07:25:15.46 Intake.open @07:25:17.07 Bypass.close @07:25:18.56 Exhaust.close @07:25:20.48 Abs.in :default @07:25:20.52 Abs.hold @07:25:20.58 PhaseSeparator.hold @07:25:20.82 SamplePump.in :charge @07:25:20.87 SamplePump.seek 2.3ml,38 seconds @07:25:27.01 Abs.stop @07:25:27.05 PhaseSeparator.stop @07:25:27.09 Intake.close @07:25:28.70 Pressurized cartridge with 0.153ml to 14.2psi @07:25:28.71 Isolated @26.4psia (12.4psi) @07:25:28.73 Verifying cartridge holds at least 0.65..0.85 of 26.4psia @07:25:28.75 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:25:35.82 Cartridge pressure fell 20% to 21.0psia @07:25:35.93 Toroid.in :hold @07:25:35.97 Toroid.seek :bypass @07:25:39.55 Toroid.in :default @07:25:39.60 Toroid.seek :clear @07:25:43.23 Cartridge::Sampler::PossibleLeak in FILTERING -- Retained only 21.0psia @07:25:43.25 Retry #1 of 2 @07:25:43.28 Cmd::SpareCartridge in FILTERING -- 2 spares remaining @07:25:43.30 Selecting Cartridge 36 @07:25:43.43 Gate.power :heat,:OFF @07:25:43.45 Gate.power :cartridge,:OFF @07:25:43.51 Carousel.in :default @07:25:43.61 Carousel.home.seek 50 @07:25:43.97 Air.in :unlocking @07:25:44.06 Air.seek :clear @07:25:45.61 Lysis1.in :unlocking @07:25:45.72 Lysis1.seek :clear @07:25:45.79 Plunge.in :unlocking @07:25:45.86 Plunge.seek :clear @07:25:46.15 Gate.power :sampler,:OFF @07:25:47.09 Carousel.seek 36 @07:25:50.26 Carousel.reconfigure :align @07:25:50.36 Lysis1.in :fast @07:25:50.41 Lysis1.seek :locked @07:25:53.06 Plunge.in :default @07:25:53.15 Plunge.seek :locked @07:25:53.23 Air.in :fast @07:25:53.28 Air.seek :locked @07:25:54.34 Carousel.reconfigure :locked @07:25:54.47 Powering cartridge bus @07:25:57.09 Gate.setIR 1750,5000,0,6 @07:25:57.21 Gate.setIR 0,5000,0,6 @07:25:57.27 PuckHeater.configure :default @07:25:57.32 Gate.power :heat,:ON,limit to 5000mA @07:25:57.45 Mounted lysis Cartridge board#J59943 @07:25:57.48 Using default cartridge heat loss curve for HeatingFunctionInCarousel @07:25:57.67 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:26:04.27 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @07:26:04.28 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @07:26:04.31 PhaseSeparator.in :filling @07:26:04.35 PhaseSeparator.hold @07:26:04.41 Gate.power :sampler,:ON @07:26:12.88 Head pressure reached 2.60psi in 9 seconds @07:26:12.90 Air.in :default @07:26:13.03 Air.seek :sealed @07:26:13.05 Priming sample loop w/20ml @07:26:13.09 SamplePump.setPosition! 0ml @07:26:13.18 Bypass.open @07:26:15.50 Exhaust.open @07:26:17.68 Bypass.close @07:26:17.72 Intake.open @07:26:20.10 Abs.in :default @07:26:20.14 Abs.hold @07:26:20.20 PhaseSeparator.in :full @07:26:20.25 PhaseSeparator.hold @07:26:20.47 Waiting for sampler to finish priming... @07:26:20.49 delayUntil "07:27:04.42" @07:27:04.48 SamplePump.in :start @07:27:04.54 SamplePump.seek 20ml,1:50 @07:27:05.17 SamplePump.configure :prime @07:27:31.14 Abs.stop @07:27:31.59 PhaseSeparator.stop @07:27:31.67 Bypass.open @07:27:33.12 Intake.close @07:27:34.81 Toroid.in :hold @07:27:34.85 Toroid.seek :crack @07:27:37.32 Toroid.seek :cartridge @07:27:39.42 Toroid.in :default @07:27:39.47 Toroid.seek :clear @07:27:43.38 Pressurizing cartridge to 14psi above 13.7psia @07:27:43.39 SamplePump.setPosition! 0ml @07:27:43.43 Intake.open @07:27:44.99 Bypass.close @07:27:46.47 Exhaust.close @07:27:48.03 Abs.in :default @07:27:48.08 Abs.hold @07:27:48.14 PhaseSeparator.hold @07:27:48.33 SamplePump.in :charge @07:27:48.38 SamplePump.seek 2.3ml,38 seconds @07:28:02.19 Abs.stop @07:28:02.23 PhaseSeparator.stop @07:28:02.28 Intake.close @07:28:03.92 Pressurized cartridge with 0.798ml to 14.2psi @07:28:03.93 Isolated @24.1psia (10.4psi) @07:28:03.95 Verifying cartridge holds at least 0.65..0.85 of 24.1psia @07:28:03.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:28:11.54 Cartridge pressure fell 23% to 18.7psia @07:28:11.65 Toroid.in :hold @07:28:11.70 Toroid.seek :bypass @07:28:15.28 Toroid.in :default @07:28:15.32 Toroid.seek :clear @07:28:18.96 Cartridge::Sampler::PossibleLeak in FILTERING -- Retained only 18.7psia @07:28:18.97 Retry #2 of 2 @07:28:19.00 Cmd::SpareCartridge in FILTERING -- 1 spare remaining @07:28:19.02 Selecting Cartridge 18 @07:28:19.16 Gate.power :heat,:OFF @07:28:19.17 Gate.power :cartridge,:OFF @07:28:19.61 Carousel.in :default @07:28:19.78 Carousel.home.seek 36 @07:28:20.15 Air.in :unlocking @07:28:20.25 Air.seek :clear @07:28:21.79 Lysis1.in :unlocking @07:28:21.91 Lysis1.seek :clear @07:28:21.96 Plunge.in :unlocking @07:28:22.03 Gate.power :sampler,:OFF @07:28:22.06 Plunge.seek :clear @07:28:23.32 Carousel.seek 18 @07:28:27.30 Carousel.reconfigure :align @07:28:27.40 Lysis1.in :fast @07:28:27.45 Lysis1.seek :locked @07:28:27.99 Slide::Warning in core -- Storage Carousel (SC) reseek at 18 + 3 counts @07:28:30.03 Plunge.in :default @07:28:30.10 Plunge.seek :locked @07:28:30.21 Air.in :fast @07:28:30.26 Air.seek :locked @07:28:31.32 Carousel.reconfigure :locked @07:28:31.45 Powering cartridge bus @07:28:34.07 Gate.setIR 1750,5000,0,6 @07:28:34.19 Gate.setIR 0,5000,0,6 @07:28:34.27 Gate.power :cartridge,:OFF @07:28:34.29 Mounted archive Cartridge @07:28:34.49 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:28:41.09 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @07:28:41.11 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @07:28:41.13 PhaseSeparator.in :filling @07:28:41.18 PhaseSeparator.hold @07:28:41.24 Gate.power :sampler,:ON @07:28:49.73 Head pressure reached 2.63psi in 9 seconds @07:28:49.80 Priming sample loop w/20ml @07:28:49.82 SamplePump.setPosition! 0ml @07:28:49.86 Bypass.open @07:28:52.20 Exhaust.open @07:28:54.36 Bypass.close @07:28:54.85 Intake.open @07:28:57.30 Abs.in :default @07:28:57.35 Abs.hold @07:28:57.41 PhaseSeparator.in :full @07:28:57.45 PhaseSeparator.hold @07:28:57.69 Waiting for sampler to finish priming... @07:28:57.70 delayUntil "07:29:41.25" @07:29:41.31 SamplePump.in :start @07:29:41.37 SamplePump.seek 20ml,1:50 @07:29:42.00 SamplePump.configure :prime @07:30:07.96 Abs.stop @07:30:08.01 PhaseSeparator.stop @07:30:08.07 Bypass.open @07:30:09.56 Intake.close @07:30:11.17 Toroid.in :hold @07:30:11.22 Toroid.seek :crack @07:30:13.68 Toroid.seek :cartridge @07:30:15.79 Toroid.in :default @07:30:15.83 Toroid.seek :clear @07:30:19.74 Pressurizing cartridge to 26psi above 13.8psia @07:30:19.76 SamplePump.setPosition! 0ml @07:30:19.79 Intake.open @07:30:21.27 Bypass.close @07:30:22.74 Exhaust.close @07:30:24.31 Abs.hold @07:30:24.38 PhaseSeparator.hold @07:30:24.57 SamplePump.in :charge @07:30:24.62 SamplePump.seek 2.3ml,40 seconds @07:30:33.22 Abs.stop @07:30:33.26 PhaseSeparator.stop @07:30:33.30 Intake.close @07:30:34.99 Pressurized cartridge with 0.305ml to 26.1psi @07:30:35.01 Isolated @35.3psia (21.5psi) @07:30:35.02 Verifying cartridge holds at least 0.65..0.85 of 35.3psia @07:30:35.04 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @07:30:42.12 Cartridge pressure fell 12% to 31.0psia @07:30:42.19 Sampling 1000.0ml @07:30:42.21 SamplePump.setPosition! 0ml @07:30:42.36 Exhaust.open @07:30:43.83 Intake.open @07:30:45.55 Abs.in :default @07:30:45.59 Abs.hold @07:30:45.66 PhaseSeparator.hold @07:30:45.92 SamplePump.in :start @07:30:45.97 SamplePump.seek 1000ml,1:07:10 @07:30:46.61 SamplePump.configure :sample @07:30:48.99 Cartridge 18 filtering 2/1000ml at 0.76ml/s with 15.5psi vacuum @07:31:49.04 Cartridge 18 filtering 48/1000ml at 0.77ml/s with 13.2psi vacuum @07:32:49.03 Cartridge 18 filtering 94/1000ml at 0.77ml/s with 15.7psi vacuum @07:33:49.03 Cartridge 18 filtering 140/1000ml at 0.76ml/s with 11.9psi vacuum @07:34:49.03 Cartridge 18 filtering 186/1000ml at 0.77ml/s with 17.7psi vacuum @07:35:49.03 Cartridge 18 filtering 232/1000ml at 0.77ml/s with 8.9psi vacuum 4.5m deep (21.3psia) @07:36:49.02 Cartridge 18 filtering 277/1000ml at 0.77ml/s with 15.2psi vacuum @07:37:49.03 Cartridge 18 filtering 323/1000ml at 0.76ml/s with 16.6psi vacuum @07:38:49.03 Cartridge 18 filtering 369/1000ml at 0.76ml/s with 17.3psi vacuum @07:39:49.03 Cartridge 18 filtering 415/1000ml at 0.76ml/s with 15.5psi vacuum @07:40:49.03 Cartridge 18 filtering 461/1000ml at 0.76ml/s with 15.1psi vacuum @07:41:49.03 Cartridge 18 filtering 506/1000ml at 0.76ml/s with 15.0psi vacuum @07:42:49.03 Cartridge 18 filtering 552/1000ml at 0.76ml/s with 19.6psi vacuum @07:43:49.03 Cartridge 18 filtering 598/1000ml at 0.76ml/s with 11.2psi vacuum @07:44:49.03 Cartridge 18 filtering 644/1000ml at 0.76ml/s with 9.8psi vacuum 4.8m deep (21.8psia) @07:45:49.03 Cartridge 18 filtering 689/1000ml at 0.76ml/s with 14.3psi vacuum @07:46:49.03 Cartridge 18 filtering 735/1000ml at 0.76ml/s with 11.3psi vacuum @07:47:49.03 Cartridge 18 filtering 781/1000ml at 0.76ml/s with 17.7psi vacuum @07:48:49.03 Cartridge 18 filtering 827/1000ml at 0.76ml/s with 11.3psi vacuum @07:49:49.03 Cartridge 18 filtering 872/1000ml at 0.76ml/s with 18.2psi vacuum @07:50:49.03 Cartridge 18 filtering 918/1000ml at 0.76ml/s with 20.6psi vacuum @07:51:49.03 Cartridge 18 filtering 964/1000ml at 0.76ml/s with 10.3psi vacuum 3.8m deep (20.3psia) @07:52:37.03 Abs.stop @07:52:37.14 PhaseSeparator.stop @07:52:37.20 Intake.close @07:52:37.22 Exhaust.close @07:52:37.42 Toroid.in :hold @07:52:37.47 Toroid.seek :bypass @07:52:41.05 Toroid.in :default @07:52:41.09 Toroid.seek :clear @07:52:44.65 Sampled 1000.0ml @07:52:45.14 Plunge.seek :pTop @07:52:46.02 Sample loop closed. Ready to Deliver Reagents @07:52:46.03 Air.in :default @07:52:46.07 Air.seek :sealed @07:52:47.75 Gate.power :sampler,:OFF @07:52:48.80 Air.in :slow @07:52:48.84 Air.seek :arEvac @07:53:01.37 Slide::Error in PROCESSING -- Air Syringe (AirS) positionErr at 1327uL (sensed 1351uL) @07:53:01.38 Retry #1 of 2 @07:53:01.40 Air.in :default @07:53:01.44 Air.hold @07:53:01.51 Air.in :slow @07:53:01.56 Air.home.seek :arEvac @07:53:02.88 Slide::Error in PROCESSING -- Air Syringe (AirS) positionErr at 1326uL (sensed 1351uL) @07:53:02.89 Retry #2 of 2 @07:53:02.91 Air.in :default @07:53:02.95 Air.hold @07:53:03.02 Air.in :slow @07:53:03.07 Air.home.seek :arEvac @07:53:04.38 Slide::Error in PROCESSING -- IGNORED Air Syringe (AirS) positionErr at 1326uL (sensed 1351uL) @07:53:04.40 Air.in :default @07:53:04.44 Air.hold @07:53:04.51 Air.in :default @07:53:04.56 Air.home.jog 0 @07:53:04.66 ArchiveError in PROCESSING -- 1326ul RNAlater undelivered @07:53:04.67 Waiting to Resume from Checkpoint "IGNORED Air Syringe (AirS) positionErr at 1326uL (sensed 1351uL)" @07:55:21.41 Session Begun @07:55:24.98 -> slots {2 => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :dry}, 18 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, 20 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}, [32,24,22] => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [50,36] => { Type: :microSPR_phyto, State: :leaky}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @07:57:19.25 -> Cart.reserve 16,18 1 @07:57:24.27 -> slots {2 => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 18 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, 20 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}, [32,24,22] => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [50,36] => { Type: :microSPR_phyto, State: :leaky}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @07:57:29.62 -> Cart.dry [2,42,32,24,22] @07:57:52.19 -> Cart.reserve 24, 22 2 @07:58:06.14 -> slots {2 => { Type: :microSPR_phyto, State: :dry}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, 18 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000.0}, 20 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}, [24,22] => { Type: :archiveHiBiomass_bac, State: :reserved, rank: 1, was: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, [50,36] => { Type: :microSPR_phyto, State: :leaky}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @07:58:14.14 -> MainThread.exit # @07:58:14.28 ThreadError in preston -- killed thread @07:58:14.33 Session Ended @07:58:29.07PDT20-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @07:58:30.36 >BREAK< ESP3 Gateway v1.44 for core bus @07:58:30.38 >BREAK< ESP3 Gateway v1.44 for cartridge bus @07:58:33.05 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @07:58:33.57 Sea pressure (Abs) tare is 6% of full scale @07:58:37.70 Polling Can every 2:00 @07:58:37.74 Loading /home/esp/esp2/bin/fireAll mission @07:58:37.95 Cartridge group: dry Spares: {spare: 1} @07:58:38.08 Gate.power :sampler,:OFF,limit to 3500mA @07:58:38.10 Gate.power :skimmer,:OFF,limit to 3500mA @07:58:38.12 Gate.power :analytic1,:ON,limit to 4000mA @07:58:38.72 Plunge.configure :default @07:58:38.76 Plunge.hold @07:58:38.82 Plunge.configure :default @07:58:39.30 SPR Module 1, FW Rev. B, FPGA Rev. C @07:58:39.35
SamplePump.configure :default @07:58:39.46 Toroid.configure :default @07:58:39.50 Toroid.hold @07:58:39.56 Toroid.configure :default @07:58:39.67 Carousel.configure :default @07:58:39.72 Carousel.hold @07:58:39.77 Carousel.configure :default @07:58:39.88 Abs.configure :default @07:58:39.99 PhaseSeparator.configure :default @07:58:40.10 Archive.configure :default @07:58:40.14 Archive.hold @07:58:40.19 Archive.configure :default @07:58:40.30 Air.configure :default @07:58:40.34 Air.hold @07:58:40.40 Air.configure :default @07:58:40.48 Lysis1.configure :default @07:58:40.52 Lysis1.hold @07:58:40.58 Lysis1.configure :default @07:58:40.66 Lysis2.configure :default @07:58:40.70 Lysis2.hold @07:58:40.76 Lysis2.configure :default @07:58:40.87 PinPort.configure :default @07:58:40.91 PinPort.hold @07:58:40.96 PinPort.configure :default @07:58:41.00 All elf controllers running firmware version 4.94 @07:58:41.58 Toroid.seek :clear @07:58:41.72
Air.seek :clear @07:58:48.54 Lysis1.seek :clear @07:58:52.56 Lysis2.seek :clear @07:58:52.63 Archive.seek :clear @07:58:52.69 Carousel.in :default @07:58:52.79 Carousel.home.seek 18 @07:58:53.07 Selecting Cartridge 2 @07:58:53.20 Carousel.in :default @07:58:53.29 Carousel.home.seek 18 @07:58:53.81 Plunge.in :unlocking @07:58:53.87 Plunge.seek :clear @07:58:55.60
Carousel.seek 2 @07:58:59.20 Carousel.reconfigure :align @07:58:59.29 Lysis1.in :fast @07:58:59.38 Lysis1.seek :locked @07:59:01.96 Plunge.in :default @07:59:02.06 Plunge.seek :locked @07:59:02.12 Air.in :fast @07:59:02.17 Air.seek :locked @07:59:03.22
Carousel.reconfigure :locked @07:59:03.35 Powering cartridge bus @07:59:05.97 Gate.setIR 1750,5000,0,6 @07:59:06.09 Gate.setIR 0,5000,0,6 @07:59:06.14 PuckHeater.configure :default @07:59:06.19 Gate.power :heat,:ON,limit to 5000mA @07:59:06.36 Mounted lysis Cartridge board#J59921 @07:59:06.39 Using default cartridge heat loss curve for HeatingFunctionInCarousel @07:59:06.63 Preparing to filter microSPR_phyto cartridge in slot 2 @07:59:06.81 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @07:59:13.41 Intake Head Pressure tare is 0% of full scale at 29C (drifted 1%) @07:59:13.42 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @07:59:13.44 PhaseSeparator.in :filling @07:59:13.48 PhaseSeparator.hold @07:59:13.54 Gate.power :sampler,:ON @07:59:21.91 Head pressure reached 2.63psi in 9 seconds @07:59:21.93 Air.in :default @07:59:22.02 Priming sample loop w/200ml, bypass w/10ml @07:59:22.05 Air.seek :sealed @07:59:22.07 SamplePump.setPosition! 0ml @07:59:22.13 Exhaust.close @07:59:22.22 Bypass.open @07:59:24.44 Intake.open @07:59:26.10 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @07:59:28.76 Vacuum tare is 54% of full scale at 29C (drifted 3%) @07:59:28.88 Abs.in :default @07:59:28.92 Abs.hold @07:59:28.98 PhaseSeparator.in :full @07:59:29.02 PhaseSeparator.hold @07:59:29.19 Waiting for sampler to finish priming... @07:59:29.20 delayUntil "08:00:13.55" @08:00:13.60 SamplePump.in :start @08:00:13.65 SamplePump.seek 10ml,1:10 @08:00:14.30 SamplePump.configure :prime @08:00:27.13 Abs.stop @08:00:27.17 PhaseSeparator.stop @08:00:27.21 SamplePump.setPosition! 0ml @08:00:27.25 Exhaust.open @08:00:29.38 Bypass.close @08:00:31.68 Abs.in :default @08:00:31.72 Abs.hold @08:00:31.78 PhaseSeparator.hold @08:00:32.02 SamplePump.in :start @08:00:32.07 SamplePump.seek 200ml,13:50 @08:00:32.71 SamplePump.configure :prime @08:02:57.67 Session Begun @08:03:00.48 -> can Can@08:03:00, 29.6C, 46% humidity, 14.7psia, 14.692V, 1.991A, 11.979Ah, 29.25W @08:04:54.61 Abs.stop @08:04:54.67 PhaseSeparator.stop @08:04:54.75 Bypass.open @08:04:56.26 Intake.close @08:04:57.96 Toroid.in :hold @08:04:58.02 Toroid.seek :crack @08:05:00.49 Toroid.seek :cartridge @08:05:02.61 Toroid.in :default @08:05:02.67 Toroid.seek :clear @08:05:06.59 Pressurizing cartridge to 14psi above 14.3psia @08:05:06.61 SamplePump.setPosition! 0ml @08:05:06.67 Intake.open @08:05:08.28 Bypass.close @08:05:09.78 Exhaust.close @08:05:11.35 Abs.in :default @08:05:11.41 Abs.hold @08:05:11.49 PhaseSeparator.hold @08:05:11.69 SamplePump.in :charge @08:05:12.16 SamplePump.seek 2.3ml,38 seconds @08:05:25.45 Abs.stop @08:05:25.51 PhaseSeparator.stop @08:05:25.57 Intake.close @08:05:27.24 Pressurized cartridge with 0.737ml to 14.7psi @08:05:27.26 Isolated @26.9psia (12.6psi) @08:05:27.29 Verifying cartridge holds at least 0.65..0.85 of 26.9psia @08:05:27.32 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:05:31.90 Cartridge pressure fell 2% to 26.5psia @08:05:31.99 Sampling 1000.0ml @08:05:32.01 SamplePump.setPosition! 0ml @08:05:32.07 Exhaust.open @08:05:33.53 Intake.open @08:05:35.26 Abs.in :default @08:05:35.30 Abs.hold @08:05:35.39 PhaseSeparator.hold @08:05:35.65 SamplePump.in :start @08:05:35.72 SamplePump.seek 1000ml,56:04 @08:05:36.35 SamplePump.configure :sample @08:05:38.73 Cartridge 2 filtering 2/1000ml at 0.64ml/s with 15.7psi vacuum @08:06:38.77 Cartridge 2 filtering 44/1000ml at 0.70ml/s with 12.7psi vacuum @08:07:38.77 Cartridge 2 filtering 85/1000ml at 0.70ml/s with 14.8psi vacuum @08:08:38.77 Cartridge 2 filtering 127/1000ml at 0.69ml/s with 11.7psi vacuum @08:09:38.77 Cartridge 2 filtering 168/1000ml at 0.69ml/s with 11.7psi vacuum @08:10:38.77 Cartridge 2 filtering 209/1000ml at 0.69ml/s with 4.1psi vacuum 9.3m deep (28.4psia) @08:11:38.77 Cartridge 2 filtering 250/1000ml at 0.68ml/s with 6.9psi vacuum 5.8m deep (23.3psia) @08:12:38.77 Cartridge 2 filtering 291/1000ml at 0.68ml/s with 12.9psi vacuum @08:13:38.77 Cartridge 2 filtering 331/1000ml at 0.67ml/s with 12.3psi vacuum @08:14:38.77 Cartridge 2 filtering 371/1000ml at 0.67ml/s with 15.4psi vacuum @08:15:38.76 Cartridge 2 filtering 411/1000ml at 0.66ml/s with 10.7psi vacuum @08:16:38.77 Cartridge 2 filtering 451/1000ml at 0.66ml/s with 5.5psi vacuum @08:17:38.77 Cartridge 2 filtering 490/1000ml at 0.66ml/s with 15.6psi vacuum @08:18:38.78 Cartridge 2 filtering 529/1000ml at 0.65ml/s with 11.5psi vacuum @08:19:39.13 Cartridge 2 filtering 568/1000ml at 0.65ml/s with 15.7psi vacuum @08:20:38.77 Cartridge 2 filtering 607/1000ml at 0.65ml/s with 13.5psi vacuum @08:21:38.77 Cartridge 2 filtering 645/1000ml at 0.64ml/s with 13.7psi vacuum @08:22:38.77 Cartridge 2 filtering 684/1000ml at 0.64ml/s with 6.8psi vacuum 5.6m deep (22.9psia) @08:23:38.78 Cartridge 2 filtering 722/1000ml at 0.64ml/s with 10.6psi vacuum @08:24:38.77 Cartridge 2 filtering 761/1000ml at 0.64ml/s with 12.4psi vacuum @08:25:38.77 Cartridge 2 filtering 799/1000ml at 0.64ml/s with 8.0psi vacuum @08:26:38.77 Cartridge 2 filtering 837/1000ml at 0.64ml/s with 10.0psi vacuum @08:27:38.77 Cartridge 2 filtering 876/1000ml at 0.64ml/s with 10.2psi vacuum @08:28:38.77 Cartridge 2 filtering 914/1000ml at 0.64ml/s with 11.1psi vacuum @08:29:38.77 Cartridge 2 filtering 952/1000ml at 0.64ml/s with 6.4psi vacuum 5.0m deep (22.1psia) @08:30:38.77 Cartridge 2 filtering 990/1000ml at 0.64ml/s with 8.7psi vacuum @08:30:54.20 Abs.stop @08:30:54.26 PhaseSeparator.stop @08:30:54.33 Intake.close @08:30:54.37 Exhaust.close @08:30:54.58 Toroid.in :hold @08:30:54.64 Toroid.seek :bypass @08:30:58.23 Toroid.in :default @08:30:58.30 Toroid.seek :clear @08:31:01.87 Sampled 1000.0ml @08:31:03.41 SPR.command "read_sequences" @08:31:03.56 SPR.command "stats_on" @08:31:03.71 SPR.command "start 0 1" @08:31:04.42 SPR.start /var/log/esp/spr26jul2008h.spr.bz2 @08:31:04.99 Gate.power :sampler,:OFF @08:31:06.93 Handoff.select :waste @08:31:07.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:31:14.06 Syringe tare is 50% of full scale at 30C (drifted -0%) @08:31:14.09 PinPort.in :locking @08:31:14.18 PinPort.seek :locked @08:31:15.01 Archive.in :fast @08:31:15.10 Lysis2.in :fast @08:31:15.13 Lysis1.seek 1250uL @08:31:15.22 Archive.seek :empty @08:31:15.31 Lysis2.seek 950uL @08:31:23.71 Evacuating puck with filter bubble point of 20psi @08:31:23.74 PinPort.seek :locked @08:31:23.84 Handoff.select :waste @08:31:23.86 Plunge.seek :puckBottom @08:31:25.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:31:31.77 Air syringe pumping up to 1 strokes to reach 4psi @08:31:31.78 Air stroke #1 of 1 @08:31:31.88 Air.in :pressurize @08:31:31.94 Air.seek :pressurized @08:31:37.22 0.6psi after pressurize @08:31:37.24 Air.in :compress @08:31:37.32 Air.seek :evac @08:31:43.91 1.5psi after compress @08:31:54.98 0.2psi after compress (stable) @08:31:55.00 Air.in :default @08:31:55.07 Air.hold @08:31:55.30 at 0.1psi Opened to waste @08:31:55.32 Handoff.select :blocked @08:31:55.46 Plunge.seek :puckTop @08:31:56.33 Air syringe pumping up to 8 strokes to reach 20psi @08:31:56.34 Air stroke #1 of 8 @08:31:56.44 Air.in :compress @08:31:56.50 Air.seek :evac @08:31:56.64 1.1psi after compress @08:32:07.21 1.4psi after compress (stable) @08:32:07.23 Air.in :gulp @08:32:07.31 Air.seek :topVent @08:32:14.10 1.3psi after gulp @08:32:19.69 1.5psi after gulp (stable) @08:32:19.70 Air stroke #2 of 8 @08:32:19.82 Air.in :pressurize @08:32:19.88 Air.seek :pressurized @08:32:26.25 2.2psi after pressurize @08:32:26.27 Air.in :compress @08:32:26.35 Air.seek :evac @08:32:32.97 14.0psi after compress @08:32:44.07 16.5psi after compress (stable) @08:32:44.09 Air.in :gulp @08:32:44.17 Air.seek :topVent @08:32:50.61 16.3psi after gulp @08:32:56.68 16.2psi after gulp (stable) @08:32:56.70 Air stroke #3 of 8 @08:32:56.80 Air.in :pressurize @08:32:56.85 Air.seek :pressurized @08:33:03.23 16.1psi after pressurize @08:33:03.27 Air.in :compress @08:33:03.36 Air.seek :evac @08:33:07.75 Reached 20.4 of 20.0psi after pumping 5199uL (2.93 strokes) @08:33:07.77 Air.in :default @08:33:07.83 Air.hold @08:33:07.91 Plunge.seek :puckBottom @08:33:08.81 delay 3 seconds @08:33:11.82 Handoff.select :waste @08:33:11.96 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @08:33:29.05 delay 1:00 @08:34:29.07 Handoff.select :blocked @08:34:29.20 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @08:34:51.77 Handoff.select :waste @08:34:51.90 0.3psi at evacEnd @08:34:51.92 Plunge.seek :pTop @08:34:52.79 delay 5 seconds @08:34:57.81 Air.in :glide @08:34:57.87 Air.seek :fullVent @08:35:00.78 delay 2 seconds @08:35:02.80 Plunge.seek :pBottom @08:35:03.67 Seawater evacuated, Pressure above/below filter released. @08:35:03.69 Plunge.seek :locked @08:35:05.00 Handoff.select :waste @08:35:05.10 Lysis1.in :slow @08:35:05.16 Lysis1.seek 950uL @08:35:19.23 delay 5 seconds @08:35:24.25 Handoff.select :blocked @08:35:24.38 Plunge.seek :puckTop @08:35:25.33 Lysis1.seek 350uL @08:35:53.32 delay 3 seconds @08:35:56.42 Air.in mix with 2.79934 parts Lysis1 @08:35:56.97 Air.seek :evac @08:35:57.04 Lysis1.seek 190uL @08:36:04.65 Air.in :fast @08:36:04.71 Air.seek :full @08:36:11.22 Lysis1.seek :empty @08:36:20.29 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @12.4psi @08:36:20.31 Plunge.seek :puckBottom @08:36:21.30 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @10.8psi @08:36:21.31 Polling Can every 2:00 @08:36:21.87 PuckHeater.configure :default #ambient 30.1C @08:36:21.95 PuckHeater.seek 81.2C @08:37:49.98 PuckHeater.hold 2:00,81.2C @08:39:50.17 Polling Can every 2:00 @08:39:50.71 PuckHeater.configure :default #ambient 30.1C @08:39:50.77 PuckHeater.seek 80.1C @08:39:50.95 PuckHeater.hold 2:00,80.1C @08:41:51.15 Polling Can every 2:00 @08:41:51.69 PuckHeater.configure :default #ambient 30.1C @08:41:51.75 PuckHeater.hold 8:00,79.1C @08:46:20.48

Session Begun @08:46:22.05 -> Cart.dry [42,32] @08:47:16.45 -> exit @08:47:16.47 Session Ended @08:49:52.13 Lysate cooked @10.6psi @08:49:52.15 Plunge.seek :puckTop @08:49:53.04 Air syringe pumping up to 5 strokes to reach 40psi @08:49:53.05 Air stroke #1 of 5 @08:49:53.15 Air.in :pressurize @08:49:53.21 Air.seek :pressurized @08:49:59.58 10.2psi after pressurize @08:49:59.60 Air.in :compress @08:49:59.67 Air.seek :evac @08:50:05.47 Reached 41.0 of 40.0psi after pumping 1707uL (0.96 strokes) @08:50:05.48 Air.in :default @08:50:05.55 Air.hold @08:50:05.63 delay 2 seconds @08:50:07.65 Air.in :fast @08:50:07.71 Air.seek :full @08:50:13.97 Plunge.seek :puckBottom @08:50:14.90 Lysis1.seek 138uL @08:50:21.45 delay 2 seconds @08:50:23.47 Plunge.seek :puckTop @08:50:24.33 Lysis1.seek :empty @08:50:30.89 Plunge.seek :puckBottom @08:50:31.93 Lysis1.in :extract @08:50:32.03 Lysis1.seek 675uL,nil @08:50:35.56 Air.in :pressurize @08:50:35.65 Air.seek :pressurized @08:50:36.50 Syringe::LysatePlungerStuck in PROCESSING -- at < 405uL during Lysis Syringe #1 (LyS1) move @08:50:36.55 Lysis1.in :default @08:50:36.62 Lysis1.seek 374uL @08:50:42.07 14.1psi after pressurize @08:50:42.09 Air.in :compressLysate @08:50:42.16 Air.seek :evac @08:50:47.70 Reached 15.7 of 15.5psi after pumping 1484uL (0.84 strokes) @08:50:47.72 Air.in :default @08:50:47.79 Air.hold @08:50:47.90 Lysis1.in :extract @08:50:48.00 Lysis1.seek 675uL,nil @08:50:49.76 Air.in :compressLysate @08:50:49.83 Air.seek :evac @08:50:56.53 Reached 17.2 of 15.5psi after pumping 1574uL (0.89 strokes) @08:50:56.55 Air.in :default @08:50:56.63 Air.hold @08:50:57.48 Air.in :compressLysate @08:50:57.53 Air.seek :evac @08:50:58.58 Air.in :default @08:50:58.64 Air.hold @08:50:58.87 Pulled 675uL lysate @13.1psi @08:50:59.03 Lysis1.seek 800uL,nil @08:50:59.24 Air.in :compressLysate @08:50:59.30 Air.seek :evac @08:51:06.02 Air.in :default @08:51:06.07 Air.hold @08:51:06.32 Air.in :glide @08:51:06.39 Air.seek :fullVent @08:51:08.96 Handoff.select :waste @08:51:09.10 Recovered 800uL lysate @15.2psi @08:51:09.13 delay 3 seconds @08:51:12.20 After 3 delay Recovered 800uL lysate @0.7psi @08:51:12.23 Plunge.seek :locked @08:51:13.55 Lysis2.in :DAmix @08:51:13.66 Lysis1.in :DAmix @08:51:14.10 Lysis2.seek 700uL @08:51:14.16 Lysis1.seek 550uL @08:51:49.11 delay 5 seconds @08:51:54.13 Handoff.select SPR @08:51:54.26 SPR.command "start 2 14" @08:51:55.46 SPR stabilized @08:51:55.60 delay 20 seconds @08:52:15.61 SPR.command "start 2 0" @08:52:18.71 Waiting up to 11:00 for SPR to enter state 1 @08:54:16.38 SPR.command "hold_on" @08:54:17.78 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @08:54:18.34 Lysis2.seek 400uL @08:54:18.39 Lysis1.seek 250uL @08:55:00.31 delay 15 seconds @08:55:15.33 SPR.command "hold_off" @08:55:18.42 Waiting up to 1:00:00 for SPR to enter state 7 @09:11:34.03 SPRsummary:50.4RIU,19.1RIU,42.2RIU,36.8RIU,high,high,high after 2566s @09:25:50.96 SPR analysis completed @09:25:55.42 Ended log spr26jul2008h.spr.bz2 @09:25:55.43 SPR.command "stats_off" @09:25:56.58 Handoff.select :close @09:25:56.73

Gate.power :heat,:OFF @09:25:56.75 Gate.power :cartridge,:OFF @09:25:56.84 PinPort.in :unlocking @09:25:56.90 PinPort.seek :clear @09:25:57.72 Carousel.in :default @09:25:57.83 Carousel.home.seek 2 @09:25:58.13 Plunge.seek :locked @09:25:58.28 Archive.in :unlocking @09:25:58.40 Archive.seek :clear @09:25:58.45 Air.in :unlocking @09:25:58.63 Air.seek :clear @09:25:58.67 Lysis1.in :unlocking @09:25:58.82 Lysis1.seek :clear @09:25:59.30 Lysis2.in :unlocking @09:25:59.37 Lysis2.seek :clear @09:26:03.47
Gate.power :motor,:OFF @09:26:03.48 Gate.power :analytic1,:OFF @09:26:03.51 Gate.power :core,:OFF @09:26:03.53 Gate.power :main,:OFF @09:26:03.55 Safely stopped and ready to power off @09:26:04.08 Gate.power :sampler,:OFF,limit to 3500mA @09:26:04.10 Gate.power :skimmer,:OFF,limit to 3500mA @09:26:07.34 Gate.power :main,:ON,limit to 5000mA @09:26:07.35 Gate.power :analytic1,:ON,limit to 4000mA @09:26:07.44 Powering core bus @09:26:09.79 SPR Module 1, FW Rev. B, FPGA Rev. C @09:26:10.17
Plunge.configure :default @09:26:10.25 SamplePump.configure :default @09:26:10.33 Toroid.configure :default @09:26:10.41 Carousel.configure :default @09:26:10.49 Abs.configure :default @09:26:10.57 PhaseSeparator.configure :full @09:26:10.64 Archive.configure :default @09:26:10.71 Air.configure :default @09:26:10.77 Lysis1.configure :default @09:26:10.83 Lysis2.configure :default @09:26:10.91 PinPort.configure :default @09:26:10.96 All elf controllers running firmware version 4.94 @09:26:10.97 Gate.power :motor,:ON,limit to 5000mA @09:26:11.21 Plunge.home.jog 0 @09:26:11.75 PinPort.home.jog 0 @09:26:11.79 Lysis2.home.jog 0 @09:26:11.83 Air.home.jog 0 @09:26:11.85 Archive.home.jog 0 @09:26:11.89 Carousel.home.jog 0 @09:26:11.92 Toroid.home.jog 0 @09:26:11.96 Lysis1.home.jog 0 @09:26:12.51 Plunge.in :homing @09:26:12.77 Plunge.jog 1485 @09:26:12.96 Lysis2.in :homing @09:26:13.00 Air.in :homing @09:26:13.04 Archive.in :homing @09:26:13.07 PinPort.in :homing @09:26:13.10 Lysis1.in :homing @09:26:13.13 Toroid.in :homing @09:26:13.29 Lysis2.jog 1617 @09:26:13.44 PinPort.jog 1210 @09:26:13.48 Air.jog 1427 @09:26:13.50 Archive.jog 1610 @09:26:13.57 Lysis1.jog 1593 @09:26:13.59 Toroid.jog 1373 @09:26:14.05 Plunge.setRawPosition! 50 @09:26:14.11 Plunge.seek :home @09:26:14.40 Plunge.in :default @09:26:14.68 Lysis2.setRawPosition! 50 @09:26:14.74 Lysis2.seek :home @09:26:14.85 Air.setRawPosition! 0 @09:26:14.91 Air.seek :home @09:26:15.04 Lysis1.setRawPosition! 50 @09:26:15.08 Air.in :default @09:26:15.11 Lysis2.in :default @09:26:15.13 Archive.setRawPosition! 50 @09:26:15.64 Lysis1.seek :home @09:26:15.73 Archive.seek :home @09:26:15.86 PinPort.setRawPosition! 3150 @09:26:15.92 PinPort.seek :home @09:26:16.04 Lysis1.in :default @09:26:16.13 Archive.in :default @09:26:18.30 Toroid.setRawPosition! 0 @09:26:18.36 Toroid.seek :home @09:26:18.45 Toroid.in :default @09:26:20.32 PinPort.in :default @09:26:20.66 Toroid.seek :clear @09:26:20.79
PinPort.in :unlocking @09:26:20.85 PinPort.seek :clear @09:26:24.27 Air.seek :clear @09:26:24.36 Lysis1.seek :clear @09:26:25.08 Lysis2.seek :clear @09:26:25.86 Archive.seek :clear @09:26:26.65 Carousel.in :default @09:26:26.76 Carousel.home.seek 2 @09:26:27.01 Selecting Cartridge 42 @09:26:27.26 Carousel.seek 42 @09:26:35.76 Carousel.reconfigure :align @09:26:35.87 Lysis1.in :fast @09:26:35.93 Lysis1.seek :locked @09:26:38.54 Plunge.seek :locked @09:26:38.67 Air.in :fast @09:26:38.74 Air.seek :locked @09:26:39.80
Carousel.reconfigure :locked @09:26:39.93 Powering cartridge bus @09:26:42.55 Gate.setIR 1750,5000,0,6 @09:26:42.67 Gate.setIR 0,5000,0,6 @09:26:42.74 PuckHeater.configure :default #ambient 30.1C @09:26:42.81 Gate.power :heat,:ON,limit to 5000mA @09:26:42.96 Mounted lysis Cartridge board#J60008 @09:26:43.00 Using default cartridge heat loss curve for HeatingFunctionInCarousel @09:26:43.25 Preparing to filter lyseHold_phyto cartridge in slot 42 @09:26:43.46 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @09:26:50.06 Intake Head Pressure tare is 0% of full scale at 30C (drifted 1%) @09:26:50.07 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @09:26:50.11 PhaseSeparator.in :filling @09:26:50.17 PhaseSeparator.hold @09:26:50.24 Gate.power :sampler,:ON @09:26:58.82 Head pressure reached 2.43psi in 9 seconds @09:26:58.84 Air.in :default @09:26:58.97 Priming sample loop w/200ml, bypass w/10ml @09:26:59.00 Air.seek :sealed @09:26:59.03 SamplePump.setPosition! 0ml @09:26:59.13 Bypass.open @09:27:01.38 Intake.open @09:27:03.09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:27:05.68 Vacuum tare is 54% of full scale at 30C (drifted 2%) @09:27:05.82 Abs.in :default @09:27:05.88 Abs.hold @09:27:05.95 PhaseSeparator.in :full @09:27:06.01 PhaseSeparator.hold @09:27:06.20 Waiting for sampler to finish priming... @09:27:06.21 delayUntil "09:27:50.26" @09:27:50.31 SamplePump.in :start @09:27:50.39 SamplePump.seek 10ml,1:10 @09:27:51.00 SamplePump.configure :prime @09:28:03.89 Abs.stop @09:28:03.95 PhaseSeparator.stop @09:28:04.00 SamplePump.setPosition! 0ml @09:28:04.06 Exhaust.open @09:28:06.26 Bypass.close @09:28:08.55 Abs.in :default @09:28:08.61 Abs.hold @09:28:08.69 PhaseSeparator.hold @09:28:08.96 SamplePump.in :start @09:28:09.02 SamplePump.seek 200ml,13:50 @09:28:09.65 SamplePump.configure :prime @09:30:51.67 -> Can.xray @09:31:09.14 Session Begun @09:31:11.35 -> Heat Puck Heater at 26C @09:31:15.00 -> can! @09:31:15.02 Polling Can every 2:00 Can@09:31:15, 29.7C, 49% humidity, 14.7psia, 14.695V, 2.016A, 13.807Ah, 29.63W @09:32:31.75 Abs.stop @09:32:31.80 PhaseSeparator.stop @09:32:31.90 Bypass.open @09:32:33.42 Intake.close @09:32:35.15 Toroid.in :hold @09:32:35.23 Toroid.seek :crack @09:32:37.71 Toroid.seek :cartridge @09:32:39.85 Toroid.in :default @09:32:39.92 Toroid.seek :clear @09:32:43.86 Pressurizing cartridge to 14psi above 14.0psia @09:32:43.88 SamplePump.setPosition! 0ml @09:32:43.96 Intake.open @09:32:45.56 Bypass.close @09:32:47.10 Exhaust.close @09:32:48.74 Abs.in :default @09:32:48.81 Abs.hold @09:32:48.90 PhaseSeparator.hold @09:32:49.09 SamplePump.in :charge @09:32:49.16 SamplePump.seek 2.3ml,38 seconds @09:33:00.13 Abs.stop @09:33:00.20 PhaseSeparator.stop @09:33:00.27 Intake.close @09:33:01.94 Pressurized cartridge with 0.498ml to 14.1psi @09:33:01.96 Isolated @26.3psia (12.3psi) @09:33:02.01 Verifying cartridge holds at least 0.65..0.85 of 26.3psia @09:33:02.05 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:33:06.64 Cartridge pressure fell 1% to 26.1psia @09:33:06.74 Sampling 1000.0ml @09:33:06.76 SamplePump.setPosition! 0ml @09:33:06.84 Exhaust.open @09:33:08.33 Intake.open @09:33:10.42 Abs.in :default @09:33:10.56 Abs.hold @09:33:10.64 PhaseSeparator.hold @09:33:10.93 SamplePump.in :start @09:33:11.02 SamplePump.seek 1000ml,56:04 @09:33:11.63 SamplePump.configure :sample @09:33:14.01 Cartridge 42 filtering 2/1000ml at 0.67ml/s with 12.3psi vacuum @09:34:14.07 Cartridge 42 filtering 43/1000ml at 0.69ml/s with 14.7psi vacuum @09:35:14.05 Cartridge 42 filtering 84/1000ml at 0.68ml/s with 11.7psi vacuum @09:36:14.04 Cartridge 42 filtering 124/1000ml at 0.67ml/s with 7.1psi vacuum 3.9m deep (20.5psia) @09:37:14.05 Cartridge 42 filtering 164/1000ml at 0.66ml/s with 9.5psi vacuum @09:38:14.04 Cartridge 42 filtering 203/1000ml at 0.65ml/s with 12.4psi vacuum @09:39:14.05 Cartridge 42 filtering 242/1000ml at 0.65ml/s with 12.2psi vacuum @09:40:14.05 Cartridge 42 filtering 281/1000ml at 0.65ml/s with 6.8psi vacuum 5.5m deep (22.8psia) @09:41:14.05 Cartridge 42 filtering 319/1000ml at 0.64ml/s with 21.7psi vacuum @09:42:14.04 Cartridge 42 filtering 357/1000ml at 0.64ml/s with 7.7psi vacuum @09:43:14.05 Cartridge 42 filtering 395/1000ml at 0.63ml/s with 3.6psi vacuum 10.2m deep (29.8psia) @09:44:14.04 Cartridge 42 filtering 433/1000ml at 0.63ml/s with 5.9psi vacuum 4.4m deep (21.2psia) @09:45:14.05 Cartridge 42 filtering 471/1000ml at 0.63ml/s with 12.6psi vacuum @09:46:14.04 Cartridge 42 filtering 508/1000ml at 0.63ml/s with 13.0psi vacuum @09:47:14.05 Cartridge 42 filtering 545/1000ml at 0.62ml/s with 6.0psi vacuum 8.7m deep (27.6psia) @09:48:14.04 Cartridge 42 filtering 583/1000ml at 0.62ml/s with 10.1psi vacuum @09:49:14.05 Cartridge 42 filtering 621/1000ml at 0.63ml/s with 11.7psi vacuum @09:50:14.04 Cartridge 42 filtering 658/1000ml at 0.63ml/s with 9.1psi vacuum @09:51:14.05 Cartridge 42 filtering 696/1000ml at 0.63ml/s with 6.0psi vacuum 6.8m deep (24.7psia) @09:52:14.04 Cartridge 42 filtering 734/1000ml at 0.63ml/s with 10.1psi vacuum @09:53:14.05 Cartridge 42 filtering 771/1000ml at 0.63ml/s with 16.7psi vacuum @09:54:14.05 Cartridge 42 filtering 809/1000ml at 0.63ml/s with 6.8psi vacuum @09:55:14.05 Cartridge 42 filtering 846/1000ml at 0.62ml/s with 10.6psi vacuum @09:56:14.05 Cartridge 42 filtering 884/1000ml at 0.62ml/s with 10.2psi vacuum @09:57:14.05 Cartridge 42 filtering 921/1000ml at 0.62ml/s with 17.0psi vacuum @09:58:14.05 Cartridge 42 filtering 957/1000ml at 0.61ml/s with 19.0psi vacuum @09:59:14.05 Cartridge 42 filtering 994/1000ml at 0.61ml/s with 9.9psi vacuum 7.2m deep (25.4psia) @09:59:23.57 Abs.stop @09:59:23.64 PhaseSeparator.stop @09:59:23.72 Intake.close @09:59:23.78 Exhaust.close @09:59:23.99 Toroid.in :hold @09:59:24.06 Toroid.seek :bypass @09:59:27.66 Toroid.in :default @09:59:27.74 Toroid.seek :clear @09:59:31.32 Sampled 1000.0ml @09:59:31.75 Handoff.select :waste @09:59:31.90 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:59:34.47 Gate.power :sampler,:OFF @09:59:38.49 Syringe tare is 50% of full scale at 30C (drifted -1%) @09:59:38.52 PinPort.in :locking @09:59:38.61 PinPort.seek :locked @09:59:39.45 Archive.in :fast @09:59:39.54 Lysis2.in :fast @09:59:39.58 Lysis1.seek 1250uL @09:59:39.62 Archive.seek :empty @09:59:39.79 Lysis2.seek :empty @09:59:48.24 Evacuating puck with filter bubble point of 20psi @09:59:48.27 PinPort.seek :locked @09:59:48.39 Handoff.select :waste @09:59:48.41 Plunge.seek :puckBottom @09:59:49.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:59:56.35 Air syringe pumping up to 1 strokes to reach 4psi @09:59:56.36 Air stroke #1 of 1 @09:59:56.48 Air.in :pressurize @09:59:56.55 Air.seek :pressurized @10:00:01.80 -0.4psi after pressurize @10:00:01.82 Air.in :compress @10:00:01.90 Air.seek :evac @10:00:08.50 1.1psi after compress @10:00:08.71 Reached 3.1 of 4.0psi after pumping 1775uL (1.00 strokes) @10:00:08.72 Air.in :default @10:00:08.81 Air.hold @10:00:08.95 Handoff.select :blocked @10:00:09.10 Plunge.seek :puckTop @10:00:09.98 Overpressure in PROCESSING -- Waste port clogged (1.4psi) @10:00:10.00 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 4psi" @10:08:07.81 -> Syringe.psi 7.47030903654741 @10:08:50.85

Session Begun @10:09:07.62 -> loop {puts Syringe.psi} @10:09:15.11 -> Handoff.waste @10:09:15.20 Handoff.select :waste Handoff Valve::Manifold selects waste @10:09:31.84 UserAbort in p -- User Abort!! @10:11:55.28 -> can! @10:11:55.31 Polling Can every 2:00 Can@10:11:55, 30.6C, 52% humidity, 14.7psia, 14.947V, 0.563A, 14.863Ah, 8.42W @10:12:04.79 -> Heat Puck Heater at 28.8C @10:13:02.30 -> can! @10:13:02.33 Polling Can every 2:00 Can@10:13:02, 30.6C, 52% humidity, 14.7psia, 14.944V, 0.567A, 14.873Ah, 8.47W @10:14:44.11 -> can! @10:14:44.13 Polling Can every 2:00 Can@10:14:44, 30.7C, 52% humidity, 14.7psia, 14.946V, 0.563A, 14.889Ah, 8.41W @10:14:47.21 -> Heat Puck Heater at 29.1C @10:16:44.68 -> TV.locked @10:16:44.71 NoMethodError in nadia -- undefined method `locked' for Toroid:Slide @10:16:52.86 -> PV.locked @10:16:52.91 Plunge.seek :locked Plunge Valve (PV) locked @10:17:15.70 -> BACsampler.filter 25 @10:17:15.91 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @10:17:22.51 Intake Head Pressure tare is 0% of full scale at 31C (drifted 1%) @10:17:22.53 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @10:17:22.61 PhaseSeparator.in :filling @10:17:22.69 PhaseSeparator.hold @10:17:22.79 Gate.power :sampler,:ON @10:17:31.37 Head pressure reached 2.48psi in 9 seconds @10:17:31.39 Air.seek :sealed @10:17:45.31 Toroid.in :hold @10:17:45.40 Toroid.seek :cartridge @10:17:49.20 Toroid.in :default @10:17:49.28 Toroid.seek :clear @10:17:53.06 Sampling 25.0ml @10:17:53.07 SamplePump.setPosition! 0ml @10:17:53.19 Exhaust.open @10:17:54.64 Intake.open @10:17:56.48 Abs.in :default @10:17:56.55 Abs.hold @10:17:56.66 PhaseSeparator.in :full @10:17:56.74 PhaseSeparator.hold @10:17:57.00 Waiting for sampler to finish priming... @10:17:57.02 delayUntil "10:18:22.81" @10:18:22.87 SamplePump.in :start @10:18:23.06 SamplePump.seek 25ml,2:10 @10:18:23.57 SamplePump.configure :sample @10:18:25.95 Cartridge 42 filtering 2/25ml at 0.76ml/s with 15.9psi vacuum @10:18:56.25 Abs.stop @10:18:56.30 PhaseSeparator.stop @10:18:56.42 Intake.close @10:18:56.51 Exhaust.close @10:18:56.59 Handoff.select :close @10:18:56.82 Toroid.in :hold @10:18:56.89 Toroid.seek :bypass @10:19:00.51 Toroid.in :default @10:19:00.61 Toroid.seek :clear @10:19:04.20 Sampled 25.0ml 25.0030893536122 @10:19:07.41 Gate.power :sampler,:OFF @10:53:59.43PDT20-Jul-26

Environmental Sample Processor built 17-Jul-26 (24b6d875) @10:54:02.81 >BREAK< ESP3 Gateway v1.44 for core bus @10:54:02.83 >BREAK< ESP3 Gateway v1.44 for cartridge bus @10:54:05.67 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @10:54:06.20 Sea pressure (Abs) tare is 6% of full scale @10:54:10.48 Polling Can every 2:00 @10:54:10.52 Waiting for espclients... @10:54:11.58 Session Begun @10:54:16.12 -> SC @10:54:16.17 BusOff in preston -- core bus was not powered while reading Storage Carousel (SC)'s status @10:54:20.09 -> ESP.ready! @10:54:20.14 Gate.power :sampler,:OFF,limit to 3500mA @10:54:20.15 Gate.power :skimmer,:OFF,limit to 3500mA @10:54:20.18 Gate.power :main,:ON,limit to 5000mA @10:54:20.20 Gate.power :analytic1,:ON,limit to 4000mA @10:54:20.34 Powering core bus @10:54:22.63 SPR Module 1, FW Rev. B, FPGA Rev. C @10:54:23.05 Plunge.configure :default @10:54:23.12 SamplePump.configure :default @10:54:23.20 Toroid.configure :default @10:54:23.28 Carousel.configure :default @10:54:23.35 Abs.configure :default @10:54:23.44 PhaseSeparator.configure :default @10:54:23.51 Archive.configure :default @10:54:23.95 Air.configure :default @10:54:24.00 Lysis1.configure :default @10:54:24.06 Lysis2.configure :default @10:54:24.14 PinPort.configure :default @10:54:24.21 All elf controllers running firmware version 4.94 @10:54:24.23 Gate.power :motor,:ON,limit to 5000mA @10:54:24.47 Plunge.home.jog 0 @10:54:24.99 PinPort.home.jog 0 @10:54:25.03 Lysis2.home.jog 0 @10:54:25.06 Air.home.jog 0 @10:54:25.09 Archive.home.jog 0 @10:54:25.12 Carousel.home.jog 0 @10:54:25.15 Toroid.home.jog 0 @10:54:25.18 Lysis1.home.jog 0 @10:54:25.73 Plunge.in :homing @10:54:25.90 Plunge.jog 1483 @10:54:26.09 PinPort.in :homing @10:54:26.12 Lysis1.in :homing @10:54:26.15 Air.in :homing @10:54:26.18 Archive.in :homing @10:54:26.21 Toroid.in :homing @10:54:26.24 Lysis2.in :homing @10:54:26.47 PinPort.jog 3150 @10:54:26.52 Lysis1.jog 4373 @10:54:26.54 Archive.jog 9650 @10:54:26.57 Toroid.jog 1363 @10:54:26.60 Air.jog 2484 @10:54:26.67 Lysis2.jog 9650 @10:54:27.17 Plunge.setRawPosition! 50 @10:54:27.22 Plunge.seek :home @10:54:27.50 Plunge.in :default @10:54:28.46 Air.setRawPosition! 0 @10:54:28.52 Air.seek :home @10:54:28.61 Air.in :default @10:54:29.24 Lysis1.setRawPosition! 50 @10:54:29.29 Lysis1.seek :home @10:54:29.57 Lysis1.in :default @10:54:30.99 PinPort.setRawPosition! 3150 @10:54:31.05 PinPort.seek :home @10:54:31.28 Toroid.setRawPosition! 0 @10:54:31.34 Toroid.seek :home @10:54:31.41 Toroid.in :default @10:54:31.75 Archive.setRawPosition! 50 @10:54:31.80 Archive.seek :home @10:54:31.88 Lysis2.setRawPosition! 50 @10:54:31.93 Lysis2.seek :home @10:54:32.08 Archive.in :default @10:54:32.20 Lysis2.in :default @10:54:35.41 PinPort.in :default @10:54:35.74 Toroid.seek :clear @10:54:35.87 PinPort.in :unlocking @10:54:35.92 PinPort.seek :clear @10:54:39.34 Air.seek :clear @10:54:39.43 Lysis1.seek :clear @10:54:40.15 Lysis2.seek :clear @10:54:40.94 Archive.seek :clear @10:54:41.72 Carousel.in :default @10:54:41.83 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @0.12psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:54:54.25 -> SC Storage Carousel (SC) at 42 @10:55:07.36 -> Cart.lock; PhytoSampler.prepareLGcart @10:55:07.45 Carousel.reconfigure :align @10:55:07.55 Lysis1.in :fast @10:55:07.61 Lysis1.seek :locked @10:55:10.22 Plunge.seek :locked @10:55:10.37 Air.in :fast @10:55:10.43 Air.seek :locked @10:55:11.49 Carousel.reconfigure :locked @10:55:11.63 Powering cartridge bus @10:55:14.25 Gate.setIR 1750,5000,0,6 @10:55:14.38 Gate.setIR 0,5000,0,6 @10:55:14.44 PuckHeater.configure :default @10:55:14.51 Gate.power :heat,:ON,limit to 5000mA @10:55:14.71 Mounted lysis Cartridge board#J60008 @10:55:14.75 Using default cartridge heat loss curve for HeatingFunctionInCarousel @10:55:14.78 Handoff.select :waste @10:55:14.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:55:21.51 Syringe tare is 50% of full scale at 24C (drifted -0%) @10:55:21.53 PinPort.in :locking @10:55:21.61 PinPort.seek :locked @10:55:22.45 Archive.in :fast @10:55:22.50 Lysis2.in :fast @10:55:22.57 Lysis1.seek 1250uL @10:55:22.64 Archive.seek :empty @10:55:22.75 Lysis2.seek 950uL Archive Syringe (ArS) empty @10:55:32.53 -> Lysis2.empty @10:55:32.56 Lysis2.in :default @10:55:32.62 Lysis2.seek :empty Lysis Syringe #2 (LyS2) empty @10:55:45.75 -> Handoff.blocked @10:55:45.78 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:55:51.35 -> Handoff.waste @10:55:51.36 Handoff.select :waste Handoff Valve::Manifold selects waste @10:55:53.20 -> Handoff.blocked @10:55:53.22 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:55:56.27 -> Handoff.waste @10:55:56.29 Handoff.select :waste Handoff Valve::Manifold selects waste @10:55:58.38 -> Handoff.blocked @10:55:58.40 Handoff.select :blocked Handoff Valve::Manifold selects blocked @10:57:25.62 -> 5.times {PhytoSampler.filter 25; PhytoSamper.evacSWpumpTo; PV.locked} @10:57:25.81 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @10:57:32.40 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @10:57:32.42 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @10:57:32.46 PhaseSeparator.in :filling @10:57:32.51 PhaseSeparator.hold @10:57:32.59 Gate.power :sampler,:ON @10:57:41.19 Head pressure reached 2.83psi in 9 seconds @10:57:41.21 Air.in :default @10:57:41.33 Air.seek :sealed @10:57:41.37 Priming sample loop w/200ml, bypass w/10ml @10:57:41.43 SamplePump.setPosition! 0ml @10:57:41.50 Bypass.open @10:57:43.78 Intake.open @10:57:45.48 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:57:48.09 Vacuum tare is 54% of full scale at 24C (drifted 2%) @10:57:48.22 Abs.in :default @10:57:48.28 Abs.hold @10:57:48.35 PhaseSeparator.in :full @10:57:48.42 PhaseSeparator.hold @10:57:48.62 Waiting for sampler to finish priming... @10:57:48.63 delayUntil "10:58:32.61" @10:58:32.65 SamplePump.in :start @10:58:32.72 SamplePump.seek 10ml,1:10 @10:58:33.35 SamplePump.configure :prime @10:58:46.22 Abs.stop @10:58:46.27 PhaseSeparator.stop @10:58:46.32 SamplePump.setPosition! 0ml @10:58:46.38 Exhaust.open @10:58:48.57 Bypass.close @10:58:50.87 Abs.in :default @10:58:50.92 Abs.hold @10:58:51.00 PhaseSeparator.hold @10:58:51.27 SamplePump.in :start @10:58:51.34 SamplePump.seek 200ml,13:50 @10:58:51.96 SamplePump.configure :prime @11:03:14.08 Abs.stop @11:03:14.13 PhaseSeparator.stop @11:03:14.27 Bypass.open @11:03:15.75 Intake.close @11:03:17.46 Toroid.in :hold @11:03:17.51 Toroid.seek :crack @11:03:20.31 Toroid.seek :cartridge @11:03:22.42 Toroid.in :default @11:03:22.49 Toroid.seek :clear @11:03:26.47 Pressurizing cartridge to 14psi above 14.5psia @11:03:26.48 SamplePump.setPosition! 0ml @11:03:26.55 Intake.open @11:03:28.12 Bypass.close @11:03:29.64 Exhaust.close @11:03:31.21 Abs.in :default @11:03:31.28 Abs.hold @11:03:31.35 PhaseSeparator.hold @11:03:31.56 SamplePump.in :charge @11:03:31.62 SamplePump.seek 2.3ml,38 seconds @11:03:42.25 Abs.stop @11:03:42.31 PhaseSeparator.stop @11:03:42.38 Intake.close @11:03:44.07 Pressurized cartridge with 0.468ml to 14.1psi @11:03:44.08 Isolated @27.7psia (13.2psi) @11:03:44.11 Verifying cartridge holds at least 0.65..0.85 of 27.7psia @11:03:44.16 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:03:48.73 Cartridge pressure fell 9% to 25.1psia @11:03:48.82 Sampling 25.0ml @11:03:48.83 SamplePump.setPosition! 0ml @11:03:48.90 Exhaust.open @11:03:50.35 Intake.open @11:03:52.16 Abs.in :default @11:03:52.22 Abs.hold @11:03:52.30 PhaseSeparator.hold @11:03:52.57 SamplePump.in :start @11:03:52.64 SamplePump.seek 25ml,1:54 @11:03:53.26 SamplePump.configure :sample @11:03:55.65 Cartridge 42 filtering 2/25ml at 0.62ml/s with 12.0psi vacuum @11:04:28.85 Abs.stop @11:04:28.90 PhaseSeparator.stop @11:04:28.97 Intake.close @11:04:29.02 Exhaust.close @11:04:29.21 Toroid.in :hold @11:04:29.27 Toroid.seek :bypass @11:04:33.29 Toroid.in :default @11:04:33.35 Toroid.seek :clear @11:04:36.92 Sampled 25.0ml @11:04:37.10 NameError in preston -- uninitialized constant PhytoSamper @11:04:40.10 Gate.power :sampler,:OFF @11:31:16.05 -> PhytoSampler.evacSWpumpTo @11:31:16.07 Evacuating puck with filter bubble point of 20psi @11:31:16.10 PinPort.seek :locked @11:31:16.20 Handoff.select :waste @11:31:16.33 Plunge.seek :puckBottom @11:31:17.63 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:31:24.21 Air syringe pumping up to 1 strokes to reach 4psi @11:31:24.22 Air stroke #1 of 1 @11:31:24.31 Air.in :pressurize @11:31:24.37 Air.seek :pressurized @11:31:29.60 -0.3psi after pressurize @11:31:29.62 Air.in :compress @11:31:29.69 Air.seek :evac @11:31:36.30 2.2psi after compress @11:31:48.37 -0.0psi after compress (stable) @11:31:48.38 Air.in :default @11:31:48.45 Air.hold @11:31:48.63 at 0.1psi Opened to waste @11:31:48.64 Handoff.select :blocked @11:31:48.78 Plunge.seek :puckTop @11:31:49.65 Air syringe pumping up to 8 strokes to reach 20psi @11:31:49.66 Air stroke #1 of 8 @11:31:49.76 Air.in :compress @11:31:49.81 Air.seek :evac @11:31:50.31 0.8psi after compress @11:32:00.88 1.3psi after compress (stable) @11:32:00.90 Air.in :gulp @11:32:00.97 Air.seek :topVent @11:32:03.26 Air.hold @11:32:03.41 Air.in :default @11:32:03.47 Air.hold @11:32:03.55 UserAbort in preston -- Air Syringe (AirS) seek to topVent Aborted! @11:32:12.15 -> Handoff.waste @11:32:12.17 Handoff.select :waste Handoff Valve::Manifold selects waste @11:32:17.67 -> PV.locked @11:32:17.71 Plunge.seek :locked Plunge Valve (PV) locked @11:33:20.45 -> Phytosampler.goalVolume = 25; PhytoSampler.primeVolme=5; PhytoSampler.bypassVolume 10 @11:33:20.48 NameError in preston -- uninitialized constant Phytosampler @11:33:31.52 -> PhytoSampler.goalVolume = 25; PhytoSampler.primeVolme=5; PhytoSampler.bypassVolume 10 @11:33:31.56 NoMethodError in preston -- undefined method `primeVolme=' for PhytoSampler:Cartridge::Sampler @11:33:40.56 -> PhytoSampler.goalVolume = 25; PhytoSampler.primeVolume=5; PhytoSampler.bypassVolume 10 @11:33:40.60 ArgumentError in preston -- wrong number of arguments (1 for 0) @11:33:47.25 -> PhytoSampler.goalVolume = 25; PhytoSampler.primeVolume=5; PhytoSampler.bypassVolume=10 10 @11:34:09.03 -> 5.times {PhytoSampler.filter 25; PhytoSampler.evacSWpumpTo; PV.locked} @11:34:09.23 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:34:15.83 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @11:34:15.84 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:34:15.89 PhaseSeparator.in :filling @11:34:15.94 PhaseSeparator.hold @11:34:16.02 Gate.power :sampler,:ON @11:34:24.51 Head pressure reached 2.68psi in 9 seconds @11:34:24.54 Air.seek :sealed @11:34:32.83 Toroid.in :hold @11:34:32.89 Toroid.seek :cartridge @11:34:36.66 Toroid.in :default @11:34:36.73 Toroid.seek :clear @11:34:40.95 Sampling 25.0ml @11:34:40.97 SamplePump.setPosition! 0ml @11:34:41.04 Exhaust.open @11:34:42.49 Intake.open @11:34:44.34 Abs.in :default @11:34:44.40 Abs.hold @11:34:44.48 PhaseSeparator.in :full @11:34:44.54 PhaseSeparator.hold @11:34:44.79 Waiting for sampler to finish priming... @11:34:44.80 delayUntil "11:35:16.04" @11:35:16.11 SamplePump.in :start @11:35:16.19 SamplePump.seek 25ml,1:54 @11:35:16.80 SamplePump.configure :sample @11:35:19.18 Cartridge 42 filtering 2/25ml at 0.54ml/s with 11.8psi vacuum @11:35:55.62 Abs.stop @11:35:55.68 PhaseSeparator.stop @11:35:55.75 Intake.close @11:35:55.79 Exhaust.close @11:35:55.86 Handoff.select :close @11:35:56.08 Toroid.in :hold @11:35:56.13 Toroid.seek :bypass @11:35:59.73 Toroid.in :default @11:35:59.80 Toroid.seek :clear @11:36:03.36 Sampled 25.0ml @11:36:03.53 Evacuating puck with filter bubble point of 20psi @11:36:03.55 PinPort.seek :locked @11:36:03.64 Handoff.select :waste @11:36:03.77 Plunge.seek :puckBottom @11:36:05.08 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:36:06.52 Gate.power :sampler,:OFF @11:36:14.16 Air syringe pumping up to 1 strokes to reach 4psi @11:36:14.17 Air stroke #1 of 1 @11:36:14.27 Air.in :pressurize @11:36:14.32 Air.seek :pressurized @11:36:19.56 0.5psi after pressurize @11:36:19.58 Air.in :compress @11:36:19.66 Air.seek :evac @11:36:22.79 Session Begun @11:36:26.26 0.3psi after compress @11:36:36.84 0.5psi after compress (stable) @11:36:36.86 Air.in :default @11:36:36.95 Air.hold @11:36:37.14 at 0.6psi Opened to waste @11:36:37.16 Handoff.select :blocked @11:36:37.32 Plunge.seek :puckTop @11:36:38.20 Air syringe pumping up to 8 strokes to reach 20psi @11:36:38.22 Air stroke #1 of 8 @11:36:38.34 Air.in :compress @11:36:38.41 Air.seek :evac @11:36:38.56 0.6psi after compress @11:36:44.47 -> PhytoSampler.pumpPrimeDelay @11:36:49.13 0.8psi after compress (stable) @11:36:49.15 Air.in :gulp @11:36:49.25 Air.seek :topVent @11:36:55.71 1.0psi after gulp @11:37:01.28 0.8psi after gulp (stable) @11:37:01.29 Air stroke #2 of 8 @11:37:01.40 Air.in :pressurize @11:37:01.46 Air.seek :pressurized @11:37:07.86 0.6psi after pressurize @11:37:07.88 Air.in :compress @11:37:07.95 Air.seek :evac @11:37:14.56 19.5psi after compress @11:37:14.85 Reached 20.7 of 20.0psi after pumping 3551uL (2.00 strokes) @11:37:14.87 Air.in :default @11:37:14.95 Air.hold @11:37:15.04 Plunge.seek :puckBottom @11:37:15.91 delay 3 seconds @11:37:18.92 Handoff.select :waste @11:37:19.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:37:33.63 delay 1:00 @11:38:33.65 Handoff.select :blocked @11:38:33.80 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:38:56.37 Handoff.select :waste @11:38:56.50 0.2psi at evacEnd @11:38:56.52 Plunge.seek :pTop @11:38:57.42 delay 5 seconds @11:39:02.44 Air.in :glide @11:39:02.50 Air.seek :fullVent @11:39:04.15 delay 2 seconds @11:39:06.18 Plunge.seek :pBottom @11:39:07.03 Seawater evacuated, Pressure above/below filter released. @11:39:07.05 Plunge.seek :locked @11:39:08.58 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:39:15.18 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @11:39:15.19 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:39:15.25 PhaseSeparator.in :filling @11:39:15.32 PhaseSeparator.hold @11:39:15.40 Gate.power :sampler,:ON @11:39:23.85 Head pressure reached 2.51psi in 9 seconds @11:39:23.88 Air.in :default @11:39:23.97 Air.seek :sealed @11:39:38.33 Toroid.in :hold @11:39:38.40 Toroid.seek :cartridge @11:39:42.25 Toroid.in :default @11:39:42.43 Toroid.seek :clear @11:39:46.24 Sampling 25.0ml @11:39:46.25 SamplePump.setPosition! 0ml @11:39:46.35 Exhaust.open @11:39:47.82 Intake.open @11:39:49.65 Abs.in :default @11:39:49.71 Abs.hold @11:39:49.80 PhaseSeparator.in :full @11:39:49.87 PhaseSeparator.hold @11:39:50.12 Waiting for sampler to finish priming... @11:39:50.13 delayUntil "11:40:15.42" @11:40:15.49 SamplePump.in :start @11:40:15.57 SamplePump.seek 25ml,1:54 @11:40:16.18 SamplePump.configure :sample @11:40:18.56 Cartridge 42 filtering 2/25ml at 0.62ml/s with 11.3psi vacuum @11:40:55.21 Abs.stop @11:40:55.27 PhaseSeparator.stop @11:40:55.36 Intake.close @11:40:55.42 Exhaust.close @11:40:55.49 Handoff.select :close @11:40:55.71 Toroid.in :hold @11:40:55.78 Toroid.seek :bypass @11:40:59.38 Toroid.in :default @11:40:59.46 Toroid.seek :clear @11:41:03.02 Sampled 25.0ml @11:41:03.21 Evacuating puck with filter bubble point of 20psi @11:41:03.24 PinPort.seek :locked @11:41:03.35 Handoff.select :waste @11:41:03.49 Plunge.seek :puckBottom @11:41:04.81 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:41:06.20 Gate.power :sampler,:OFF @11:41:11.38 Air syringe pumping up to 1 strokes to reach 4psi @11:41:11.39 Air stroke #1 of 1 @11:41:11.51 Air.in :pressurize @11:41:11.58 Air.seek :pressurized @11:41:16.82 0.3psi after pressurize @11:41:16.85 Air.in :compress @11:41:16.93 Air.seek :evac @11:41:24.01 0.3psi after compress @11:41:34.58 0.2psi after compress (stable) @11:41:34.60 Air.in :default @11:41:34.69 Air.hold @11:41:34.88 at 0.3psi Opened to waste @11:41:34.90 Handoff.select :blocked @11:41:35.06 Plunge.seek :puckTop @11:41:35.92 Air syringe pumping up to 8 strokes to reach 20psi @11:41:35.93 Air stroke #1 of 8 @11:41:36.06 Air.in :compress @11:41:36.13 Air.seek :evac @11:41:36.27 0.3psi after compress @11:41:46.84 0.3psi after compress (stable) @11:41:46.87 Air.in :gulp @11:41:46.96 Air.seek :topVent @11:41:53.42 0.2psi after gulp @11:41:58.99 0.4psi after gulp (stable) @11:41:59.00 Air stroke #2 of 8 @11:41:59.13 Air.in :pressurize @11:41:59.19 Air.seek :pressurized @11:42:05.56 0.0psi after pressurize @11:42:05.58 Air.in :compress @11:42:05.67 Air.seek :evac @11:42:12.28 18.5psi after compress @11:42:12.52 Reached 19.7 of 20.0psi after pumping 3551uL (2.00 strokes) @11:42:12.54 Air.in :default @11:42:12.62 Air.hold @11:42:12.71 Plunge.seek :puckBottom @11:42:13.59 delay 3 seconds @11:42:16.60 Handoff.select :waste @11:42:16.74 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:42:29.31 delay 1:00 @11:43:29.34 Handoff.select :blocked @11:43:29.49 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:43:52.06 Handoff.select :waste @11:43:52.20 0.3psi at evacEnd @11:43:52.22 Plunge.seek :pTop @11:43:53.11 delay 5 seconds @11:43:58.13 Air.in :glide @11:43:58.19 Air.seek :fullVent @11:43:59.83 delay 2 seconds @11:44:01.86 Plunge.seek :pBottom @11:44:02.73 Seawater evacuated, Pressure above/below filter released. @11:44:02.75 Plunge.seek :locked @11:44:04.24 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:44:10.83 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @11:44:10.85 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:44:10.92 PhaseSeparator.in :filling @11:44:10.98 PhaseSeparator.hold @11:44:11.06 Gate.power :sampler,:ON @11:44:19.56 Head pressure reached 2.69psi in 9 seconds @11:44:19.59 Air.in :default @11:44:19.68 Air.seek :sealed @11:44:34.04 Toroid.in :hold @11:44:34.11 Toroid.seek :cartridge @11:44:37.90 Toroid.in :default @11:44:37.96 Toroid.seek :clear @11:44:41.73 Sampling 25.0ml @11:44:41.74 SamplePump.setPosition! 0ml @11:44:41.83 Exhaust.open @11:44:43.27 Intake.open @11:44:45.13 Abs.in :default @11:44:45.19 Abs.hold @11:44:45.28 PhaseSeparator.in :full @11:44:45.35 PhaseSeparator.hold @11:44:46.08 Waiting for sampler to finish priming... @11:44:46.10 delayUntil "11:45:11.08" @11:45:11.16 SamplePump.in :start @11:45:11.24 SamplePump.seek 25ml,1:54 @11:45:11.85 SamplePump.configure :sample @11:45:14.23 Cartridge 42 filtering 2/25ml at 0.59ml/s with 12.2psi vacuum @11:45:51.32 Abs.stop @11:45:51.37 PhaseSeparator.stop @11:45:51.47 Intake.close @11:45:51.52 Exhaust.close @11:45:51.58 Handoff.select :close @11:45:51.85 Toroid.in :hold @11:45:51.91 Toroid.seek :bypass @11:45:55.50 Toroid.in :default @11:45:55.58 Toroid.seek :clear @11:45:59.16 Sampled 25.0ml @11:45:59.34 Evacuating puck with filter bubble point of 20psi @11:45:59.37 PinPort.seek :locked @11:45:59.48 Handoff.select :waste @11:45:59.61 Plunge.seek :puckBottom @11:46:00.92 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:46:02.34 Gate.power :sampler,:OFF @11:46:07.50 Air syringe pumping up to 1 strokes to reach 4psi @11:46:07.51 Air stroke #1 of 1 @11:46:07.62 Air.in :pressurize @11:46:07.69 Air.seek :pressurized @11:46:12.93 0.3psi after pressurize @11:46:12.96 Air.in :compress @11:46:13.05 Air.seek :evac @11:46:19.66 0.3psi after compress @11:46:30.23 0.2psi after compress (stable) @11:46:30.24 Air.in :default @11:46:30.33 Air.hold @11:46:30.52 at 0.4psi Opened to waste @11:46:30.53 Handoff.select :blocked @11:46:30.69 Plunge.seek :puckTop @11:46:31.57 Air syringe pumping up to 8 strokes to reach 20psi @11:46:31.58 Air stroke #1 of 8 @11:46:31.69 Air.in :compress @11:46:31.76 Air.seek :evac @11:46:31.90 0.4psi after compress @11:46:42.47 0.4psi after compress (stable) @11:46:42.49 Air.in :gulp @11:46:42.58 Air.seek :topVent @11:46:49.05 0.4psi after gulp @11:46:54.62 0.5psi after gulp (stable) @11:46:54.63 Air stroke #2 of 8 @11:46:54.74 Air.in :pressurize @11:46:54.81 Air.seek :pressurized @11:47:01.26 0.3psi after pressurize @11:47:01.29 Air.in :compress @11:47:01.38 Air.seek :evac @11:47:08.00 18.4psi after compress @11:47:08.20 Reached 19.6 of 20.0psi after pumping 3551uL (2.00 strokes) @11:47:08.22 Air.in :default @11:47:08.30 Air.hold @11:47:08.40 Plunge.seek :puckBottom @11:47:09.75 delay 3 seconds @11:47:12.78 Handoff.select :waste @11:47:12.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:47:22.50 delay 1:00 @11:48:22.53 Handoff.select :blocked @11:48:22.68 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:48:49.25 Handoff.select :waste @11:48:49.39 -1.6psi at evacEnd @11:48:49.41 Plunge.seek :pTop @11:48:50.30 delay 5 seconds @11:48:55.33 Air.in :glide @11:48:55.39 Air.seek :fullVent @11:48:57.04 delay 2 seconds @11:48:59.06 Plunge.seek :pBottom @11:48:59.92 Seawater evacuated, Pressure above/below filter released. @11:48:59.94 Plunge.seek :locked @11:49:01.43 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:49:08.03 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @11:49:08.05 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:49:08.11 PhaseSeparator.in :filling @11:49:08.18 PhaseSeparator.hold @11:49:08.26 Gate.power :sampler,:ON @11:49:16.75 Head pressure reached 2.71psi in 9 seconds @11:49:16.78 Air.in :default @11:49:16.87 Air.seek :sealed @11:49:31.23 Toroid.in :hold @11:49:31.29 Toroid.seek :cartridge @11:49:35.06 Toroid.in :default @11:49:35.12 Toroid.seek :clear @11:49:38.89 Sampling 25.0ml @11:49:38.90 SamplePump.setPosition! 0ml @11:49:38.99 Exhaust.open @11:49:40.47 Intake.open @11:49:42.25 Abs.in :default @11:49:42.31 Abs.hold @11:49:42.40 PhaseSeparator.in :full @11:49:42.46 PhaseSeparator.hold @11:49:42.73 Waiting for sampler to finish priming... @11:49:42.74 delayUntil "11:50:08.27" @11:50:08.36 SamplePump.in :start @11:50:08.44 SamplePump.seek 25ml,1:54 @11:50:09.05 SamplePump.configure :sample @11:50:11.44 Cartridge 42 filtering 2/25ml at 0.54ml/s with 10.9psi vacuum @11:50:48.23 Abs.stop @11:50:48.30 PhaseSeparator.stop @11:50:48.39 Intake.close @11:50:48.45 Exhaust.close @11:50:48.52 Handoff.select :close @11:50:48.75 Toroid.in :hold @11:50:48.83 Toroid.seek :bypass @11:50:52.43 Toroid.in :default @11:50:52.51 Toroid.seek :clear @11:50:56.10 Sampled 25.0ml @11:50:56.28 Evacuating puck with filter bubble point of 20psi @11:50:56.31 PinPort.seek :locked @11:50:56.42 Handoff.select :waste @11:50:56.55 Plunge.seek :puckBottom @11:50:57.86 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:50:59.28 Gate.power :sampler,:OFF @11:51:04.44 Air syringe pumping up to 1 strokes to reach 4psi @11:51:04.45 Air stroke #1 of 1 @11:51:04.56 Air.in :pressurize @11:51:04.63 Air.seek :pressurized @11:51:09.88 0.4psi after pressurize @11:51:09.90 Air.in :compress @11:51:10.00 Air.seek :evac @11:51:16.60 0.1psi after compress @11:51:27.18 0.4psi after compress (stable) @11:51:27.20 Air.in :default @11:51:27.29 Air.hold @11:51:27.48 at 0.4psi Opened to waste @11:51:27.49 Handoff.select :blocked @11:51:27.65 Plunge.seek :puckTop @11:51:28.52 Air syringe pumping up to 8 strokes to reach 20psi @11:51:28.53 Air stroke #1 of 8 @11:51:28.64 Air.in :compress @11:51:28.72 Air.seek :evac @11:51:28.86 0.3psi after compress @11:51:39.43 0.3psi after compress (stable) @11:51:39.45 Air.in :gulp @11:51:39.55 Air.seek :topVent @11:51:46.01 0.5psi after gulp @11:51:51.58 0.3psi after gulp (stable) @11:51:51.59 Air stroke #2 of 8 @11:51:51.71 Air.in :pressurize @11:51:51.78 Air.seek :pressurized @11:51:58.17 0.3psi after pressurize @11:51:58.19 Air.in :compress @11:51:58.28 Air.seek :evac @11:52:04.90 18.9psi after compress @11:52:05.11 Reached 20.2 of 20.0psi after pumping 3551uL (2.00 strokes) @11:52:05.12 Air.in :default @11:52:05.21 Air.hold @11:52:05.30 Plunge.seek :puckBottom @11:52:06.66 delay 3 seconds @11:52:09.69 Handoff.select :waste @11:52:09.82 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:52:20.41 delay 1:00 @11:53:20.43 Handoff.select :blocked @11:53:20.57 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:53:43.14 Handoff.select :waste @11:53:43.29 0.4psi at evacEnd @11:53:43.31 Plunge.seek :pTop @11:53:44.21 delay 5 seconds @11:53:49.23 Air.in :glide @11:53:49.30 Air.seek :fullVent @11:53:50.95 delay 2 seconds @11:53:52.97 Plunge.seek :pBottom @11:53:53.85 Seawater evacuated, Pressure above/below filter released. @11:53:53.87 Plunge.seek :locked @11:53:55.36 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @11:54:01.96 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @11:54:01.98 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @11:54:02.04 PhaseSeparator.in :filling @11:54:02.11 PhaseSeparator.hold @11:54:02.19 Gate.power :sampler,:ON @11:54:10.66 Head pressure reached 2.47psi in 9 seconds @11:54:10.69 Air.in :default @11:54:10.78 Air.seek :sealed @11:54:25.14 Toroid.in :hold @11:54:25.22 Toroid.seek :cartridge @11:54:29.00 Toroid.in :default @11:54:29.06 Toroid.seek :clear @11:54:32.82 Sampling 25.0ml @11:54:32.84 SamplePump.setPosition! 0ml @11:54:32.93 Exhaust.open @11:54:34.41 Intake.open @11:54:36.21 Abs.in :default @11:54:36.27 Abs.hold @11:54:36.36 PhaseSeparator.in :full @11:54:36.43 PhaseSeparator.hold @11:54:36.68 Waiting for sampler to finish priming... @11:54:36.70 delayUntil "11:55:02.20" @11:55:02.27 SamplePump.in :start @11:55:02.35 SamplePump.seek 25ml,1:54 @11:55:02.96 SamplePump.configure :sample @11:55:05.34 Cartridge 42 filtering 2/25ml at 0.58ml/s with 12.7psi vacuum @11:55:42.57 Abs.stop @11:55:42.65 PhaseSeparator.stop @11:55:42.74 Intake.close @11:55:42.80 Exhaust.close @11:55:42.87 Handoff.select :close @11:55:43.11 Toroid.in :hold @11:55:43.18 Toroid.seek :bypass @11:55:46.79 Toroid.in :default @11:55:46.85 Toroid.seek :clear @11:55:50.44 Sampled 25.0ml @11:55:50.62 Evacuating puck with filter bubble point of 20psi @11:55:50.65 PinPort.seek :locked @11:55:50.76 Handoff.select :waste @11:55:50.90 Plunge.seek :puckBottom @11:55:52.21 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:55:53.62 Gate.power :sampler,:OFF @11:55:58.79 Air syringe pumping up to 1 strokes to reach 4psi @11:55:58.80 Air stroke #1 of 1 @11:55:58.92 Air.in :pressurize @11:55:58.99 Air.seek :pressurized @11:56:04.25 -0.1psi after pressurize @11:56:04.27 Air.in :compress @11:56:04.36 Air.seek :evac @11:56:10.97 -0.0psi after compress @11:56:21.54 0.2psi after compress (stable) @11:56:21.55 Air.in :default @11:56:21.64 Air.hold @11:56:22.32 at 0.2psi Opened to waste @11:56:22.33 Handoff.select :blocked @11:56:22.49 Plunge.seek :puckTop @11:56:23.36 Air syringe pumping up to 8 strokes to reach 20psi @11:56:23.37 Air stroke #1 of 8 @11:56:23.50 Air.in :compress @11:56:23.57 Air.seek :evac @11:56:23.71 -0.1psi after compress @11:56:34.28 0.2psi after compress (stable) @11:56:34.30 Air.in :gulp @11:56:34.40 Air.seek :topVent @11:56:40.86 0.2psi after gulp @11:56:46.43 0.1psi after gulp (stable) @11:56:46.44 Air stroke #2 of 8 @11:56:46.56 Air.in :pressurize @11:56:46.63 Air.seek :pressurized @11:56:53.02 -0.1psi after pressurize @11:56:53.05 Air.in :compress @11:56:53.14 Air.seek :evac @11:56:59.75 17.6psi after compress @11:56:59.95 Reached 18.8 of 20.0psi after pumping 3551uL (2.00 strokes) @11:56:59.97 Air.in :default @11:57:00.05 Air.hold @11:57:00.15 Plunge.seek :puckBottom @11:57:01.03 delay 3 seconds @11:57:04.04 Handoff.select :waste @11:57:04.17 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:57:13.75 delay 1:00 @11:58:13.77 Handoff.select :blocked @11:58:13.92 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @11:58:36.49 Handoff.select :waste @11:58:36.62 0.3psi at evacEnd @11:58:36.64 Plunge.seek :pTop @11:58:37.53 delay 5 seconds @11:58:42.56 Air.in :glide @11:58:42.62 Air.seek :fullVent @11:58:44.75 delay 2 seconds @11:58:46.80 Plunge.seek :pBottom @11:58:47.67 Seawater evacuated, Pressure above/below filter released. @11:58:47.70 Plunge.seek :locked 5 @12:02:37.70 -> to 50 @12:02:37.86 Gate.power :heat,:OFF @12:02:37.88 Gate.power :cartridge,:OFF @12:02:37.99 PinPort.in :unlocking @12:02:38.05 PinPort.seek :clear @12:02:38.89 Carousel.in :default @12:02:39.01 Carousel.home.seek 42 @12:02:39.34 Archive.in :unlocking @12:02:39.46 Archive.seek :clear @12:02:39.56 Air.in :unlocking @12:02:39.70 Air.seek :clear @12:02:39.74 Lysis2.in :unlocking @12:02:39.88 Lysis2.seek :clear @12:02:44.60 Lysis1.in :unlocking @12:02:44.73 Lysis1.seek :clear @12:02:44.77 Plunge.in :unlocking @12:02:44.89 Plunge.seek :clear @12:02:46.99 Carousel.seek 50 @12:02:48.95 Carousel.reconfigure :align @12:02:49.07 Lysis1.in :fast @12:02:49.13 Lysis1.seek :locked @12:02:51.73 Plunge.in :default @12:02:51.83 Plunge.seek :locked @12:02:51.95 Air.in :fast @12:02:52.02 Air.seek :locked @12:02:53.10 Carousel.reconfigure :locked @12:02:53.25 Powering cartridge bus @12:02:55.87 Gate.setIR 1750,5000,0,6 @12:02:56.00 Gate.setIR 0,5000,0,6 @12:02:56.08 PuckHeater.configure :default @12:02:56.15 Gate.power :heat,:ON,limit to 5000mA @12:02:56.31 Mounted lysis Cartridge board#J59986 @12:02:56.36 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @12:03:04.02 -> Sampler.reset @12:03:15.17 -> PhytoSampler.primeVolume 200 @12:03:15.20 ArgumentError in preston -- wrong number of arguments (1 for 0) @12:03:18.66 -> PhytoSampler.primeVolume=200 200 @12:03:24.08 -> PhytoSampler.bypassVolume=200 200 @12:03:27.56 -> PhytoSampler.bypassVolume=50 50 @12:03:50.38 -> PhytoSampler.filter 300 @12:03:51.06 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:03:57.67 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @12:03:57.68 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @12:03:57.74 PhaseSeparator.in :filling @12:03:57.81 PhaseSeparator.hold @12:03:57.89 Gate.power :sampler,:ON @12:04:06.37 Head pressure reached 2.71psi in 9 seconds @12:04:06.39 Air.in :default @12:04:06.53 Air.seek :sealed @12:04:06.57 Priming sample loop w/200ml, bypass w/50ml @12:04:06.63 SamplePump.setPosition! 0ml @12:04:06.71 Bypass.open @12:04:08.99 Intake.open @12:04:10.66 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:04:13.26 Vacuum tare is 54% of full scale at 24C (drifted 2%) @12:04:13.40 Abs.in :default @12:04:13.47 Abs.hold @12:04:13.55 PhaseSeparator.in :full @12:04:13.62 PhaseSeparator.hold @12:04:13.82 Waiting for sampler to finish priming... @12:04:13.83 delayUntil "12:04:57.91" @12:04:57.97 SamplePump.in :start @12:04:58.05 SamplePump.seek 50ml,3:50 @12:04:58.66 SamplePump.configure :prime @12:06:04.02 Abs.stop @12:06:04.09 PhaseSeparator.stop @12:06:04.16 SamplePump.setPosition! 0ml @12:06:04.22 Exhaust.open @12:06:06.37 Bypass.close @12:06:08.68 Abs.in :default @12:06:08.74 Abs.hold @12:06:08.83 PhaseSeparator.hold @12:06:09.11 SamplePump.in :start @12:06:09.19 SamplePump.seek 200ml,13:50 @12:06:09.81 SamplePump.configure :prime @12:06:27.77 -> loop {puts Sampler.psi; Delay.sleep 0.5} @12:10:32.41 Abs.stop @12:10:32.55 PhaseSeparator.stop @12:10:32.68 Bypass.open @12:10:34.18 Intake.close @12:10:35.80 Toroid.in :hold @12:10:35.87 Toroid.seek :crack @12:10:38.35 Toroid.seek :cartridge @12:10:40.48 Toroid.in :default @12:10:40.55 Toroid.seek :clear @12:10:44.48 Pressurizing cartridge to 14psi above 15.1psia @12:10:44.49 SamplePump.setPosition! 0ml @12:10:44.56 Intake.open @12:10:46.16 Bypass.close @12:10:47.69 Exhaust.close @12:10:49.75 Abs.in :default @12:10:49.86 Abs.hold @12:10:49.95 PhaseSeparator.hold @12:10:50.23 SamplePump.in :charge @12:10:50.29 SamplePump.seek 2.3ml,38 seconds @12:10:57.98 Abs.stop @12:10:58.05 PhaseSeparator.stop @12:10:58.12 Intake.close @12:10:59.80 Pressurized cartridge with 0.241ml to 14.0psi @12:10:59.81 Isolated @24.6psia (9.5psi) @12:10:59.86 Verifying cartridge holds at least 0.65..0.85 of 24.6psia @12:10:59.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:11:04.58 Cartridge pressure fell 8% to 22.7psia @12:11:04.68 Sampling 300.0ml @12:11:04.69 SamplePump.setPosition! 0ml @12:11:04.77 Exhaust.open @12:11:06.24 Intake.open @12:11:08.07 Abs.in :default @12:11:08.14 Abs.hold @12:11:08.22 PhaseSeparator.hold @12:11:09.09 SamplePump.in :start @12:11:09.19 SamplePump.seek 300ml,17:10 @12:11:09.78 SamplePump.configure :sample @12:11:12.22 Cartridge 50 filtering 2/300ml at 0.56ml/s with 11.7psi vacuum @12:12:12.31 Cartridge 50 filtering 40/300ml at 0.64ml/s with 12.7psi vacuum @12:13:12.26 Cartridge 50 filtering 78/300ml at 0.63ml/s with 10.8psi vacuum @12:14:12.26 Cartridge 50 filtering 115/300ml at 0.63ml/s with 15.3psi vacuum @12:15:12.26 Cartridge 50 filtering 154/300ml at 0.64ml/s with 10.1psi vacuum @12:16:04.58 UserAbort in preston#2 -- User Abort!! @12:16:12.28 Cartridge 50 filtering 197/300ml at 0.72ml/s with 7.9psi vacuum 7.1m deep (25.2psia) @12:17:12.27 Cartridge 50 filtering 240/300ml at 0.70ml/s with 12.1psi vacuum @12:18:12.27 Cartridge 50 filtering 281/300ml at 0.69ml/s with 14.1psi vacuum @12:18:40.72 Abs.stop @12:18:40.79 PhaseSeparator.stop @12:18:40.87 Intake.close @12:18:40.94 Exhaust.close @12:18:41.00 Handoff.select :close @12:18:41.24 Toroid.in :hold @12:18:41.32 Toroid.seek :bypass @12:18:44.92 Toroid.in :default @12:18:45.00 Toroid.seek :clear @12:18:48.59 Sampled 300.0ml 300.0 @12:18:51.77 Gate.power :sampler,:OFF @12:18:57.04 -> to 36 @12:18:57.21 Gate.power :heat,:OFF @12:18:57.23 Gate.power :cartridge,:OFF @12:18:57.33 Carousel.in :default @12:18:57.45 Carousel.home.seek 50 @12:18:58.37 Air.in :unlocking @12:18:58.50 Air.seek :clear @12:19:00.07 Lysis1.in :unlocking @12:19:00.20 Lysis1.seek :clear @12:19:00.30 Plunge.in :unlocking @12:19:00.38 Plunge.seek :clear @12:19:01.54 Carousel.seek 36 @12:19:04.72 Carousel.reconfigure :align @12:19:04.84 Lysis1.in :fast @12:19:04.90 Lysis1.seek :locked @12:19:07.50 Plunge.in :default @12:19:07.56 Plunge.seek :locked @12:19:07.70 Air.in :fast @12:19:07.78 Air.seek :locked @12:19:08.85 Carousel.reconfigure :locked @12:19:08.99 Powering cartridge bus @12:19:11.62 Gate.setIR 1750,5000,0,6 @12:19:11.75 Gate.setIR 0,5000,0,6 @12:19:11.82 PuckHeater.configure :default @12:19:11.90 Gate.power :heat,:ON,limit to 5000mA @12:19:12.07 Mounted lysis Cartridge board#J59943 @12:19:12.10 Using default cartridge heat loss curve for HeatingFunctionInCarousel :microSPR_phyto @12:19:12.22 -> Cart.leaky [36] @12:19:23.59 -> PhytoSampler.filter 300 @12:19:23.79 Waiting up to 3:00 for Intake Head Pressure to stabilize +/-0.02psi @12:19:30.38 Intake Head Pressure tare is 0% of full scale at 24C (drifted 1%) @12:19:30.40 Waiting up to 2:00 for Intake Head Pressure to reach 1.30psi @12:19:30.46 PhaseSeparator.in :filling @12:19:30.51 PhaseSeparator.hold @12:19:30.59 Gate.power :sampler,:ON @12:19:39.14 Head pressure reached 2.69psi in 9 seconds @12:19:39.17 Air.in :default @12:19:39.31 Air.seek :sealed @12:19:39.35 Bypass.open @12:19:41.03 Toroid.in :hold @12:19:41.10 Toroid.seek :crack @12:19:43.58 Toroid.seek :cartridge @12:19:45.71 Toroid.in :default @12:19:45.79 Toroid.seek :clear @12:19:49.54 Exhaust.open @12:19:51.08 Intake.open @12:19:52.79 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:19:55.38 Vacuum tare is 54% of full scale at 24C (drifted 2%) @12:19:55.52 Pressurizing cartridge to 14psi above 15.1psia @12:19:55.53 SamplePump.setPosition! 0ml @12:19:55.61 Bypass.close @12:19:57.10 Exhaust.close @12:19:58.67 Abs.in :default @12:19:58.74 Abs.hold @12:19:58.82 PhaseSeparator.in :full @12:19:58.89 PhaseSeparator.hold @12:19:59.08 Waiting for sampler to finish priming... @12:19:59.09 delayUntil "12:20:30.61" @12:20:30.65 SamplePump.in :charge @12:20:30.74 SamplePump.seek 2.3ml,38 seconds @12:20:40.66 Abs.stop @12:20:40.73 PhaseSeparator.stop @12:20:40.80 Intake.close @12:20:42.51 Pressurized cartridge with 0.406ml to 14.2psi @12:20:42.52 Isolated @27.1psia (12.0psi) @12:20:42.57 Verifying cartridge holds at least 0.65..0.85 of 27.1psia @12:20:42.62 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:20:47.28 Sampling 300.0ml @12:20:47.30 SamplePump.setPosition! 0ml @12:20:47.39 Exhaust.open @12:20:48.87 Intake.open @12:20:50.66 Abs.in :default @12:20:50.72 Abs.hold @12:20:50.81 PhaseSeparator.hold @12:20:51.08 SamplePump.in :start @12:20:51.16 SamplePump.seek 300ml,17:10 @12:20:51.78 SamplePump.configure :sample @12:20:54.65 Cartridge 36 filtering 2/300ml at 0.65ml/s with 12.2psi vacuum @12:21:54.20 Cartridge 36 filtering 45/300ml at 0.72ml/s with 11.5psi vacuum @12:22:54.21 Cartridge 36 filtering 88/300ml at 0.71ml/s with 10.1psi vacuum @12:23:54.20 Cartridge 36 filtering 129/300ml at 0.69ml/s with 6.9psi vacuum 3.9m deep (20.4psia) @12:24:54.21 Cartridge 36 filtering 170/300ml at 0.68ml/s with 11.8psi vacuum @12:25:54.20 Cartridge 36 filtering 210/300ml at 0.67ml/s with 15.0psi vacuum @12:25:57.24 -> Cart.dry [32] @12:26:05.27 -> Cart.type 32 @12:26:05.29 ArgumentError in preston#2 -- wrong number of arguments (1 for 0) @12:26:13.64 -> Cart.reserved [16,24,22] @12:26:33.93 -> slots {2 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul2008h.spr.bz2"}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [20,18] => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}, [24,22] => { Type: :archiveHiBiomass_bac, State: :reserved, rank: 1, was: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 36 => { Type: :microSPR_phyto, State: :filtering, TV: :open}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :paused, filtered: 1000, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :paused}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @12:26:54.21 Cartridge 36 filtering 250/300ml at 0.67ml/s with 9.5psi vacuum @12:27:54.20 Cartridge 36 filtering 290/300ml at 0.66ml/s with 11.4psi vacuum @12:28:10.23 Abs.stop @12:28:10.30 PhaseSeparator.stop @12:28:10.38 Intake.close @12:28:10.43 Exhaust.close @12:28:10.63 Toroid.in :hold @12:28:10.70 Toroid.seek :bypass @12:28:14.30 Toroid.in :default @12:28:14.38 Toroid.seek :clear @12:28:17.96 Sampled 300.0ml 300.0 @12:28:21.14 Gate.power :sampler,:OFF @12:37:20.06 -> MainThread.exit # @12:37:20.20 ThreadError in preston#2 -- killed thread @12:37:20.26 Session Ended @12:37:20.31 Session Ended @14:06:44.77PDT20-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @14:06:48.23 >BREAK< ESP3 Gateway v1.44 for core bus @14:06:48.25 >BREAK< ESP3 Gateway v1.44 for cartridge bus @14:06:51.23 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @14:06:51.79 Sea pressure (Abs) tare is 6% of full scale @14:06:56.40 Polling Can every 2:00 @14:06:56.43 Waiting for espclients... @14:06:57.53 Session Begun @14:07:16.05 -> ESP.ready!; Sampler.refill @14:07:16.09 Gate.power :main,:ON,limit to 5000mA @14:07:16.12 Gate.power :analytic1,:ON,limit to 4000mA @14:07:16.14 Gate.power :analytic2,:ON,limit to 4000mA @14:07:16.16 Gate.power :analytic3,:ON,limit to 4000mA @14:07:16.31 Powering core bus @14:07:18.57 SPR Module 1, FW Rev. B, FPGA Rev. C @14:07:19.02 Plunge.configure :default @14:07:19.10 SamplePump.configure :default @14:07:19.18 Toroid.configure :default @14:07:19.25 Carousel.configure :default @14:07:19.71 Abs.configure :default @14:07:19.79 PhaseSeparator.configure :default @14:07:19.88 Archive.configure :default @14:07:19.96 Air.configure :default @14:07:20.01 Lysis1.configure :default @14:07:20.06 Lysis2.configure :default @14:07:20.15 PinPort.configure :default @14:07:20.20 All elf controllers running firmware version 4.94 @14:07:20.23 Gate.power :motor,:ON,limit to 5000mA @14:07:20.42 Plunge.home.jog 0 @14:07:20.98 PinPort.home.jog 0 @14:07:21.02 Lysis2.home.jog 0 @14:07:21.06 Air.home.jog 0 @14:07:21.09 Archive.home.jog 0 @14:07:21.12 Toroid.home.jog 0 @14:07:21.15 Lysis1.home.jog 0 @14:07:21.18 Carousel.home.jog 0 @14:07:21.80 Plunge.in :homing @14:07:21.96 Lysis2.in :homing @14:07:22.01 Air.in :homing @14:07:22.04 Archive.in :homing @14:07:22.07 Plunge.jog 1479 @14:07:22.11 PinPort.in :homing @14:07:22.14 Lysis1.in :homing @14:07:22.17 Toroid.in :homing @14:07:22.43 Lysis2.jog 1615 @14:07:22.46 Archive.jog 1618 @14:07:22.49 Lysis1.jog 2262 @14:07:22.52 Air.jog 2469 @14:07:22.55 PinPort.jog 1281 @14:07:22.61 Toroid.jog 1365 @14:07:23.54 Plunge.setRawPosition! 50 @14:07:23.59 Plunge.seek :home @14:07:23.84 Lysis2.setRawPosition! 50 @14:07:23.92 Plunge.in :default @14:07:23.95 Lysis2.seek :home @14:07:24.08 Archive.setRawPosition! 50 @14:07:24.13 Archive.seek :home @14:07:24.28 Lysis2.in :default @14:07:24.30 Lysis1.setRawPosition! 50 @14:07:24.42 Lysis1.seek :home @14:07:24.51 Air.setRawPosition! 0 @14:07:24.55 Archive.in :default @14:07:24.62 Air.seek :home @14:07:24.76 PinPort.setRawPosition! 3150 @14:07:24.78 Lysis1.in :default @14:07:24.81 Air.in :default @14:07:24.93 PinPort.seek :home @14:07:27.29 Toroid.setRawPosition! 0 @14:07:27.35 Toroid.seek :home @14:07:27.43 Toroid.in :default @14:07:29.33 PinPort.in :default @14:07:29.66 Toroid.seek :clear @14:07:29.79 PinPort.in :unlocking @14:07:29.86 PinPort.seek :clear @14:07:33.30 Air.seek :clear @14:07:33.40 Lysis1.seek :clear @14:07:34.12 Lysis2.seek :clear @14:07:34.90 Archive.seek :clear @14:07:35.69 Carousel.in :default @14:07:35.80 Carousel.home.seek 36 @14:07:36.37 Bypass.open @14:07:37.89 Intake.open @14:07:39.53 SamplePump.setPosition! 0ml @14:07:39.64 SamplePump.seek 30ml @14:08:19.58 Bypass.close @14:08:21.14 Exhaust.open @14:08:22.68 SamplePump.seek 55ml @14:08:56.07 delay 1 second @14:08:57.09 Intake.close @14:08:57.15 Exhaust.close Sample Pump (SP) at 55ml @14:10:24.09 -> PhytoSampler.prime 40, 10 @14:10:24.17 Priming sample loop w/40ml, bypass w/10ml @14:10:24.19 SamplePump.setPosition! 0ml @14:10:24.25 Bypass.open @14:10:26.53 Intake.open @14:10:28.20 Sea 22.5m deep (47.9psia) @14:10:28.22 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:10:30.83 Vacuum tare is 64% of full scale at 23C (drifted 12%) @14:10:30.96 Intake.close @14:10:31.02 Bypass.close @14:10:32.62 Verifying toroid holds at least 0.6..0.85 of 47.8psia @14:10:32.64 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @14:10:37.22 Bypass.open @14:10:38.73 Intake.open @14:10:41.09 Abs.in :default @14:10:41.15 Abs.hold @14:10:41.36 SamplePump.in :start @14:10:41.43 SamplePump.seek 10ml,1:10 @14:10:42.05 SamplePump.configure :prime @14:10:54.95 Abs.stop @14:10:55.01 SamplePump.setPosition! 0ml @14:10:55.07 Exhaust.open @14:10:57.19 Bypass.close @14:10:59.52 Abs.in :default @14:10:59.57 Abs.hold @14:10:59.69 Sea 22.4m deep (47.8psia) @14:10:59.85 SamplePump.in :start @14:10:59.92 SamplePump.seek 40ml,3:10 @14:11:00.55 SamplePump.configure :prime @14:11:52.82 Abs.stop PhytoSampler @14:12:20.71 -> SC Storage Carousel (SC) at 36 @14:12:32.55 -> slot 36 {36 => { Type: :microSPR_phyto, State: :paused}} @14:12:41.81 -> slot 36=>:dry {36 => { Type: :microSPR_phyto, State: :dry}} @14:13:07.56 -> Cart.dry [36,32] @14:13:33.64 -> slot 32 {32 => { Type: :archiveHiBiomass_bac, State: :dry, rank: 1}} @14:13:49.80 -> start {fire archive, lysis} # @14:13:50.51 Toroid.seek :clear @14:13:50.67 Air.seek :clear @14:13:50.76 Lysis1.seek :clear @14:13:50.86 Lysis2.seek :clear @14:13:50.94 Archive.seek :clear @14:13:51.03 Carousel.in :default @14:13:51.16 Carousel.home.seek 36 @14:13:51.42 Selecting Cartridge 32 @14:13:51.67 Carousel.seek 32 @14:13:52.82 Carousel.reconfigure :align @14:13:52.95 Lysis1.in :fast @14:13:53.01 Lysis1.seek :locked @14:13:53.10 Slide::Warning in core -- Storage Carousel (SC) reseek at 32 + 4 counts (sensed 32 + 5 counts) @14:13:55.62 Plunge.seek :locked @14:13:55.76 Air.in :fast @14:13:55.82 Air.seek :locked @14:13:56.88 Carousel.reconfigure :locked @14:13:57.02 Powering cartridge bus @14:13:59.65 Gate.setIR 1750,5000,0,6 @14:13:59.77 Gate.setIR 0,5000,0,6 @14:13:59.86 Gate.power :cartridge,:OFF @14:13:59.90 Mounted archive Cartridge @14:14:00.17 Preparing to filter archiveHiBiomass_bac cartridge in slot 32 @14:14:00.42 Priming sample loop w/200ml, bypass w/10ml @14:14:00.44 SamplePump.setPosition! 0ml @14:14:00.51 Exhaust.close @14:14:02.00 Bypass.open @14:14:04.51 Intake.close @14:14:04.59 Bypass.close @14:14:06.21 Verifying toroid holds at least 0.6..0.85 of 47.7psia @14:14:06.23 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @14:14:10.83 Toroid pressure rose 1% to 48.0psia @14:14:10.84 Bypass.open @14:14:12.39 Intake.open @14:14:14.78 Abs.in :default @14:14:14.84 Abs.hold @14:14:15.09 SamplePump.in :start @14:14:15.16 SamplePump.seek 10ml,1:10 @14:14:15.79 SamplePump.configure :prime @14:14:28.68 Abs.stop @14:14:28.73 SamplePump.setPosition! 0ml @14:14:28.79 Exhaust.open @14:14:30.96 Bypass.close @14:14:33.33 Abs.in :default @14:14:33.40 Abs.hold @14:14:33.52 Sea 22.7m deep (48.3psia) @14:14:33.69 SamplePump.in :start @14:14:33.76 SamplePump.seek 200ml,13:50 @14:14:34.39 SamplePump.configure :prime @14:18:56.63 Abs.stop @14:18:56.71 Bypass.open @14:18:58.25 Intake.close @14:18:59.90 Toroid.in :hold @14:18:59.96 Toroid.seek :crack @14:19:02.42 Toroid.seek :cartridge @14:19:04.54 Toroid.in :default @14:19:04.61 Toroid.seek :clear @14:19:08.54 Exhaust.close @14:19:08.60 Bypass.close @14:19:10.20 Verifying cartridge holds at least 0.65..0.85 of 48.1psia @14:19:10.22 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @14:19:14.80 Cartridge pressure fell 1% to 47.6psia @14:19:14.89 Sampling 1000.0ml @14:19:14.90 SamplePump.setPosition! 0ml @14:19:14.97 Exhaust.open @14:19:16.80 Intake.open @14:19:18.58 Abs.in :default @14:19:18.64 Abs.hold @14:19:18.77 Sea 22.7m deep (48.2psia) @14:19:18.94 SamplePump.in :start @14:19:19.01 SamplePump.seek 1000ml,1:07:10 @14:19:19.64 SamplePump.configure :sample @14:19:22.02 Cartridge 32 filtering 2/1000ml at 0.76ml/s with 14.7psi vacuum 14.2m deep (35.7psia) @14:20:22.07 Cartridge 32 filtering 48/1000ml at 0.76ml/s with 12.7psi vacuum 21.2m deep (46.0psia) @14:21:22.07 Cartridge 32 filtering 94/1000ml at 0.76ml/s with 7.1psi vacuum 21.1m deep (45.9psia) @14:22:22.07 Cartridge 32 filtering 139/1000ml at 0.76ml/s with 13.6psi vacuum 27.6m deep (55.5psia) @14:23:22.07 Cartridge 32 filtering 185/1000ml at 0.76ml/s with 9.6psi vacuum 21.4m deep (46.3psia) @14:24:22.07 Cartridge 32 filtering 231/1000ml at 0.76ml/s with 8.0psi vacuum 29.7m deep (58.5psia) @14:25:22.08 Cartridge 32 filtering 276/1000ml at 0.76ml/s with 18.3psi vacuum 21.1m deep (45.8psia) @14:26:22.07 Cartridge 32 filtering 322/1000ml at 0.76ml/s with 18.6psi vacuum 21.7m deep (46.7psia) @14:27:22.07 Cartridge 32 filtering 368/1000ml at 0.76ml/s with 7.2psi vacuum 31.0m deep (60.6psia) @14:28:22.07 Cartridge 32 filtering 413/1000ml at 0.76ml/s with 12.0psi vacuum 20.7m deep (45.3psia) @14:29:22.07 Cartridge 32 filtering 459/1000ml at 0.76ml/s with 18.5psi vacuum 20.0m deep (44.2psia) @14:30:22.07 Cartridge 32 filtering 504/1000ml at 0.76ml/s with 11.9psi vacuum 19.3m deep (43.3psia) @14:31:22.07 Cartridge 32 filtering 550/1000ml at 0.76ml/s with 15.2psi vacuum 25.6m deep (52.5psia) @14:32:22.07 Cartridge 32 filtering 595/1000ml at 0.76ml/s with 24.6psi vacuum 14.9m deep (36.7psia) @14:33:22.07 Cartridge 32 filtering 641/1000ml at 0.76ml/s with 7.1psi vacuum 30.6m deep (59.9psia) @14:34:22.07 Cartridge 32 filtering 686/1000ml at 0.76ml/s with 19.9psi vacuum 18.8m deep (42.4psia) @14:35:22.07 Cartridge 32 filtering 731/1000ml at 0.76ml/s with 21.0psi vacuum 16.9m deep (39.6psia) @14:36:22.07 Cartridge 32 filtering 777/1000ml at 0.76ml/s with 13.5psi vacuum 18.3m deep (41.7psia) @14:37:22.07 Cartridge 32 filtering 822/1000ml at 0.76ml/s with 8.5psi vacuum 29.4m deep (58.1psia) @14:38:22.07 Cartridge 32 filtering 868/1000ml at 0.76ml/s with 14.4psi vacuum 24.7m deep (51.2psia) @14:39:22.08 Cartridge 32 filtering 913/1000ml at 0.76ml/s with 16.2psi vacuum 28.6m deep (57.0psia) @14:40:22.07 Cartridge 32 filtering 958/1000ml at 0.76ml/s with 24.0psi vacuum 16.6m deep (39.2psia) @14:41:17.04 Abs.stop @14:41:17.12 Intake.close @14:41:17.19 Exhaust.close @14:41:17.36 Toroid.in :hold @14:41:17.41 Toroid.seek :bypass @14:41:21.00 Toroid.in :default @14:41:21.06 Toroid.seek :clear @14:41:24.63 Sampled 1000.0ml @14:41:25.56 Plunge.seek :pTop @14:41:26.44 Sample loop closed. Ready to Deliver Reagents @14:41:26.46 Air.in :default @14:41:26.54 Air.seek :sealed @14:41:29.28 Air.in :slow @14:41:29.35 Air.seek :arEvac @14:42:43.90 delay 1:00 @14:43:43.94 Air.in :default @14:43:44.00 Air.seek :clear @14:44:02.67 RNAlater soak for 10:00 @14:50:04.72 -> can! @14:50:04.75 Polling Can every 2:00 Can@14:50:05, 21.9C, 46% humidity, 14.7psia, 14.967V, 0.457A, 0.625Ah, 6.84W @14:50:09.22 -> can! @14:50:09.25 Polling Can every 2:00 Can@14:50:09, 63% Wet!, 21.9C, 46% humidity, 14.7psia, 14.966V, 0.456A, 0.626Ah, 6.83W @14:54:02.70 Air.seek :tVent @14:54:05.17 delay 1 second @14:54:06.19 Air.in :halfSpeed @14:54:06.25 Air.seek :arEmpty @14:54:38.94 delay 10 seconds @14:54:48.96 delay 1:00 @14:55:48.99 Plunge.seek :pTop @14:55:49.07 Sample preserved and evacuated @14:55:49.19 Selecting Cartridge 36 @14:55:49.39 Carousel.in :default @14:55:49.51 Carousel.home.seek 32 @14:55:49.89 Air.in :unlocking @14:55:49.98 Air.seek :clear @14:55:54.75 Lysis1.in :unlocking @14:55:54.86 Lysis1.seek :clear @14:55:54.95 Plunge.in :unlocking @14:55:55.02 Plunge.seek :clear @14:55:56.46 Carousel.seek 36 @14:55:57.62 Carousel.reconfigure :align @14:55:57.73 Lysis1.in :fast @14:55:57.79 Lysis1.seek :locked @14:56:00.38 Plunge.in :default @14:56:00.47 Plunge.seek :locked @14:56:00.58 Air.in :fast @14:56:00.64 Air.seek :locked @14:56:01.71 Carousel.reconfigure :locked @14:56:01.85 Powering cartridge bus @14:56:04.47 Gate.setIR 1750,5000,0,6 @14:56:04.60 Gate.setIR 0,5000,0,6 @14:56:04.66 PuckHeater.configure :default @14:56:04.73 Gate.power :heat,:ON,limit to 5000mA @14:56:04.93 Mounted lysis Cartridge board#J59943 @14:56:04.97 Using default cartridge heat loss curve for HeatingFunctionInCarousel @14:56:05.23 Preparing to filter microSPR_phyto cartridge in slot 36 @14:56:05.86 Air.in :default @14:56:05.96 Air.seek :sealed @14:56:06.06 Priming sample loop w/200ml, bypass w/10ml @14:56:06.08 SamplePump.setPosition! 0ml @14:56:06.14 Bypass.open @14:56:08.43 Intake.open @14:56:10.09 Sea 22.6m deep (48.0psia) @14:56:10.11 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @14:56:12.72 Vacuum tare is 64% of full scale at 22C (drifted 12%) @14:56:12.85 Intake.close @14:56:12.91 Bypass.close @14:56:14.50 Verifying toroid holds at least 0.6..0.85 of 47.8psia @14:56:14.52 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @14:56:19.11 Bypass.open @14:56:20.61 Intake.open @14:56:22.92 Abs.in :default @14:56:22.99 Abs.hold @14:56:23.19 SamplePump.in :start @14:56:23.26 SamplePump.seek 10ml,1:10 @14:56:23.88 SamplePump.configure :prime @14:56:36.77 Abs.stop @14:56:36.83 SamplePump.setPosition! 0ml @14:56:36.88 Exhaust.open @14:56:39.07 Bypass.close @14:56:41.40 Abs.in :default @14:56:41.46 Abs.hold @14:56:41.58 Sea 22.5m deep (48.0psia) @14:56:41.75 SamplePump.in :start @14:56:41.82 SamplePump.seek 200ml,13:50 @14:56:42.44 SamplePump.configure :prime @15:01:04.51 Abs.stop @15:01:04.59 Bypass.open @15:01:06.09 Intake.close @15:01:07.80 Toroid.in :hold @15:01:07.86 Toroid.seek :crack @15:01:10.33 Toroid.seek :cartridge @15:01:12.45 Toroid.in :default @15:01:12.52 Toroid.seek :clear @15:01:16.50 Exhaust.close @15:01:16.54 Bypass.close @15:01:18.11 Verifying cartridge holds at least 0.65..0.85 of 48.1psia @15:01:18.13 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @15:01:25.72 Cartridge pressure fell 10% to 43.3psia @15:01:25.81 Sampling 1000.0ml @15:01:25.83 SamplePump.setPosition! 0ml @15:01:25.90 Exhaust.open @15:01:27.40 Intake.open @15:01:29.13 Abs.in :default @15:01:29.20 Abs.hold @15:01:29.33 Sea 22.6m deep (48.0psia) @15:01:29.51 SamplePump.in :start @15:01:29.58 SamplePump.seek 1000ml,56:04 @15:01:30.20 SamplePump.configure :sample @15:01:32.59 Cartridge 36 filtering 2/1000ml at 0.54ml/s with 10.9psi vacuum 22.0m deep (47.1psia) @15:02:32.63 Cartridge 36 filtering 40/1000ml at 0.64ml/s with 10.3psi vacuum 25.8m deep (52.8psia) @15:03:32.63 Cartridge 36 filtering 78/1000ml at 0.64ml/s with 7.7psi vacuum 24.6m deep (51.1psia) @15:04:32.63 Cartridge 36 filtering 116/1000ml at 0.64ml/s with 15.9psi vacuum 17.4m deep (40.4psia) @15:05:32.64 Cartridge 36 filtering 154/1000ml at 0.63ml/s with 15.4psi vacuum 20.2m deep (44.6psia) @15:06:32.63 Cartridge 36 filtering 192/1000ml at 0.63ml/s with 7.9psi vacuum 27.1m deep (54.8psia) @15:07:32.64 Cartridge 36 filtering 230/1000ml at 0.63ml/s with 10.2psi vacuum 21.0m deep (45.8psia) @15:08:32.63 Cartridge 36 filtering 268/1000ml at 0.63ml/s with 10.2psi vacuum 22.3m deep (47.7psia) @15:09:32.63 Cartridge 36 filtering 305/1000ml at 0.62ml/s with 14.7psi vacuum 22.3m deep (47.7psia) @15:10:32.63 Cartridge 36 filtering 343/1000ml at 0.62ml/s with 9.4psi vacuum 27.3m deep (55.0psia) @15:11:32.63 Cartridge 36 filtering 380/1000ml at 0.62ml/s with 15.8psi vacuum 21.5m deep (46.5psia) @15:12:32.63 Cartridge 36 filtering 417/1000ml at 0.62ml/s with 14.0psi vacuum 20.7m deep (45.3psia) @15:13:32.63 Cartridge 36 filtering 454/1000ml at 0.62ml/s with 10.5psi vacuum 21.5m deep (46.5psia) @15:14:32.63 Cartridge 36 filtering 491/1000ml at 0.62ml/s with 9.4psi vacuum 26.3m deep (53.6psia) @15:14:50.60 -> slot {2 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul2008h.spr.bz2"}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [20,18] => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}, [24,22] => { Type: :archiveHiBiomass_bac, State: :reserved, rank: 1, was: :dry}, 32 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 36 => { Type: :microSPR_phyto, State: :filtering, TV: :open}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 41 => { Type: :archive_bac, State: :missing}, 42 => { Type: :lyseHold_phyto, State: :paused, filtered: 1000, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :paused}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @15:15:32.62 Cartridge 36 filtering 528/1000ml at 0.62ml/s with 10.7psi vacuum 20.9m deep (45.6psia) @15:16:32.63 Cartridge 36 filtering 565/1000ml at 0.62ml/s with 9.6psi vacuum 23.4m deep (49.2psia) @15:16:39.66 -> slot 18 {18 => { Type: :archiveHiBiomass_bac, State: :preserving, filtered: 1000}} @15:17:13.43 -> slot 18=>nil {18 => nil} @15:17:22.19 -> Cart.processing [] @15:17:32.63 Cartridge 36 filtering 602/1000ml at 0.62ml/s with 10.8psi vacuum 23.9m deep (50.0psia) @15:17:34.39 -> cart.preserving @15:17:34.42 NoMethodError in preston -- undefined method `preserving' for :cart:Symbol @15:17:40.49 -> Cart.preserving [20] @15:17:51.41 -> slot 20=>nil {20 => nil} @15:18:32.63 Cartridge 36 filtering 639/1000ml at 0.62ml/s with 15.7psi vacuum 20.2m deep (44.6psia) @15:19:32.63 Cartridge 36 filtering 676/1000ml at 0.62ml/s with 14.2psi vacuum 21.4m deep (46.3psia) @15:20:19.57 -> Cart.lyseHold_phyto [40,42] @15:20:28.53 -> slot 42 {42 => { Type: :lyseHold_phyto, State: :paused, filtered: 1000, rank: 1}} @15:20:32.63 Cartridge 36 filtering 713/1000ml at 0.62ml/s with 9.6psi vacuum 26.5m deep (53.9psia) @15:20:46.17 -> slot 40 {40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}} @15:21:32.63 Cartridge 36 filtering 750/1000ml at 0.61ml/s with 8.7psi vacuum 23.8m deep (49.9psia) @15:22:32.63 Cartridge 36 filtering 787/1000ml at 0.61ml/s with 12.7psi vacuum 21.5m deep (46.5psia) @15:23:32.63 Cartridge 36 filtering 824/1000ml at 0.61ml/s with 11.4psi vacuum 19.6m deep (43.7psia) @15:24:32.64 Cartridge 36 filtering 861/1000ml at 0.61ml/s with 6.3psi vacuum 23.2m deep (49.0psia) @15:25:32.63 Cartridge 36 filtering 897/1000ml at 0.61ml/s with 11.5psi vacuum 18.3m deep (41.8psia) @15:26:32.63 Cartridge 36 filtering 934/1000ml at 0.61ml/s with 10.7psi vacuum 21.1m deep (45.9psia) @15:27:32.63 Cartridge 36 filtering 971/1000ml at 0.61ml/s with 12.6psi vacuum 24.0m deep (50.2psia) @15:28:19.79 Abs.stop @15:28:19.86 Intake.close @15:28:19.92 Exhaust.close @15:28:20.12 Toroid.in :hold @15:28:20.19 Toroid.seek :bypass @15:28:23.79 Toroid.in :default @15:28:23.86 Toroid.seek :clear @15:28:27.44 Sampled 1000.0ml @15:28:28.96 SPR.command "read_sequences" @15:28:29.67 SPR.command "stats_on" @15:28:29.80 SPR.command "start 0 1" @15:28:30.53 SPR.start /var/log/esp/spr26jul2015h.spr.bz2 @15:28:33.04 Handoff.select :waste @15:28:33.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:28:40.79 Syringe tare is 51% of full scale at 22C (drifted -0%) @15:28:40.81 PinPort.in :locking @15:28:40.90 PinPort.seek :locked @15:28:41.74 Archive.in :fast @15:28:41.79 Lysis2.in :fast @15:28:41.85 Lysis1.seek 1250uL @15:28:41.96 Archive.seek :empty @15:28:42.08 Lysis2.seek 950uL @15:28:50.45 Evacuating puck with filter bubble point of 20psi @15:28:50.47 PinPort.seek :locked @15:28:50.58 Handoff.select :waste @15:28:50.60 Plunge.seek :puckBottom @15:28:51.93 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:28:58.52 Air syringe pumping up to 1 strokes to reach 4psi @15:28:58.56 Air stroke #1 of 1 @15:28:58.68 Air.in :pressurize @15:28:58.73 Air.seek :pressurized @15:29:03.96 -0.2psi after pressurize @15:29:03.98 Air.in :compress @15:29:04.06 Air.seek :evac @15:29:10.66 1.0psi after compress @15:29:21.28 0.3psi after compress (stable) @15:29:21.31 Air.in :default @15:29:21.38 Air.hold @15:29:21.57 at 0.2psi Opened to waste @15:29:21.58 Handoff.select :blocked @15:29:21.73 Plunge.seek :puckTop @15:29:22.60 Air syringe pumping up to 8 strokes to reach 20psi @15:29:22.61 Air stroke #1 of 8 @15:29:22.72 Air.in :compress @15:29:22.78 Air.seek :evac @15:29:22.92 1.6psi after compress @15:29:33.49 1.8psi after compress (stable) @15:29:33.52 Air.in :gulp @15:29:33.62 Air.seek :topVent @15:29:40.07 1.7psi after gulp @15:29:45.65 1.7psi after gulp (stable) @15:29:45.66 Air stroke #2 of 8 @15:29:45.76 Air.in :pressurize @15:29:45.84 Air.seek :pressurized @15:29:52.23 1.8psi after pressurize @15:29:52.25 Air.in :compress @15:29:52.33 Air.seek :evac @15:29:58.94 13.7psi after compress @15:30:11.02 17.3psi after compress (stable) @15:30:11.04 Air.in :gulp @15:30:11.12 Air.seek :topVent @15:30:17.56 16.8psi after gulp @15:30:23.14 17.0psi after gulp (stable) @15:30:23.15 Air stroke #3 of 8 @15:30:23.26 Air.in :pressurize @15:30:23.32 Air.seek :pressurized @15:30:29.70 16.5psi after pressurize @15:30:29.72 Air.in :compress @15:30:29.80 Air.seek :evac @15:30:34.17 Reached 20.3 of 20.0psi after pumping 5195uL (2.93 strokes) @15:30:34.19 Air.in :default @15:30:34.27 Air.hold @15:30:34.36 Plunge.seek :puckBottom @15:30:35.23 delay 3 seconds @15:30:38.24 Handoff.select :waste @15:30:38.38 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:30:51.46 delay 1:00 @15:31:51.47 Handoff.select :blocked @15:31:51.62 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @15:32:14.20 Handoff.select :waste @15:32:14.34 0.0psi at evacEnd @15:32:14.39 Plunge.seek :pTop @15:32:15.26 delay 5 seconds @15:32:20.29 Air.in :glide @15:32:20.35 Air.seek :fullVent @15:32:23.30 delay 2 seconds @15:32:25.32 Plunge.seek :pBottom @15:32:26.20 Seawater evacuated, Pressure above/below filter released. @15:32:26.22 Plunge.seek :locked @15:32:27.54 Handoff.select :waste @15:32:27.67 Lysis1.in :slow @15:32:27.74 Lysis1.seek 950uL @15:32:41.82 delay 5 seconds @15:32:46.83 Handoff.select :blocked @15:32:46.97 Plunge.seek :puckTop @15:32:47.92 Lysis1.seek 350uL @15:33:15.91 delay 3 seconds @15:33:18.98 Air.in mix with 2.79934 parts Lysis1 @15:33:19.53 Air.seek :evac @15:33:19.61 Lysis1.seek 190uL @15:33:27.24 Air.in :fast @15:33:27.31 Air.seek :full @15:33:33.82 Lysis1.seek :empty @15:33:42.91 Lysis Buffer Delivered. Plunge Valve (PV) at puckTop Syringe pressure @9.0psi @15:33:42.93 Plunge.seek :puckBottom @15:33:43.91 Lysis Buffer Delivered. Plunge Valve (PV) at puckBottom Syringe pressure @7.7psi @15:33:43.93 Polling Can every 2:00 @15:33:44.48 PuckHeater.configure :default #ambient 21.6C @15:33:44.55 PuckHeater.seek 81.7C @15:35:16.35 PuckHeater.hold 2:00,81.7C @15:37:16.22 Polling Can every 2:00 @15:37:16.75 PuckHeater.configure :default #ambient 21.9C @15:37:16.81 PuckHeater.seek 80.6C @15:37:16.96 PuckHeater.hold 2:00,80.6C @15:39:16.94 Polling Can every 2:00 @15:39:17.47 PuckHeater.configure :default #ambient 21.9C @15:39:17.53 PuckHeater.hold 8:00,79.6C @15:47:16.78 Lysate cooked @9.1psi @15:47:16.80 Plunge.seek :puckTop @15:47:17.70 Air syringe pumping up to 5 strokes to reach 40psi @15:47:17.71 Air stroke #1 of 5 @15:47:17.81 Air.in :pressurize @15:47:17.87 Air.seek :pressurized @15:47:24.25 9.0psi after pressurize @15:47:24.29 Air.in :compress @15:47:24.37 Air.seek :evac @15:47:29.58 Reached 41.0 of 40.0psi after pumping 1693uL (0.95 strokes) @15:47:29.59 Air.in :default @15:47:29.66 Air.hold @15:47:29.75 delay 2 seconds @15:47:31.77 Air.in :fast @15:47:31.85 Air.seek :full @15:47:38.07 Plunge.seek :puckBottom @15:47:39.01 Lysis1.seek 138uL @15:47:45.55 delay 2 seconds @15:47:47.57 Plunge.seek :puckTop @15:47:48.44 Lysis1.seek :empty @15:47:54.99 Plunge.seek :puckBottom @15:47:56.07 Lysis1.in :extract @15:47:56.15 Lysis1.seek 675uL,nil @15:48:00.32 Air.in :pressurize @15:48:00.39 Air.seek :pressurized @15:48:00.80 Syringe::LysatePlungerStuck in PROCESSING -- at < 474uL during Lysis Syringe #1 (LyS1) move @15:48:00.84 Lysis1.in :default @15:48:00.91 Lysis1.seek 444uL @15:48:06.85 12.5psi after pressurize @15:48:06.87 Air.in :compressLysate @15:48:06.97 Air.seek :evac @15:48:11.64 Reached 15.5 of 15.5psi after pumping 1471uL (0.83 strokes) @15:48:11.66 Air.in :default @15:48:11.74 Air.hold @15:48:11.88 Lysis1.in :extract @15:48:11.95 Lysis1.seek 675uL,nil @15:48:13.91 Air.in :compressLysate @15:48:13.97 Syringe::LysatePlungerStuck in PROCESSING -- at < 545uL during Lysis Syringe #1 (LyS1) move @15:48:14.04 Lysis1.in :default @15:48:14.11 Air.seek :evac @15:48:14.18 Lysis1.seek 515uL @15:48:15.65 Reached 16.0 of 15.5psi after pumping 1490uL (0.84 strokes) @15:48:15.67 Air.in :default @15:48:15.75 Air.hold @15:48:15.98 Lysis1.in :extract @15:48:16.04 Lysis1.seek 675uL,nil @15:48:17.75 Air.in :compressLysate @15:48:17.82 Air.seek :evac @15:48:18.12 Air.in :default @15:48:18.19 Air.hold @15:48:18.41 Pulled 675uL lysate @12.3psi @15:48:18.56 Lysis1.seek 800uL,nil @15:48:18.78 Air.in :compressLysate @15:48:18.84 Air.seek :evac @15:48:27.31 Syringe::LysatePlungerStuck in PROCESSING -- at < 769uL during Lysis Syringe #1 (LyS1) move @15:48:27.34 Lysis1.in :default @15:48:27.42 Lysis1.seek 739uL @15:48:28.15 Reached 16.0 of 15.5psi after pumping 1624uL (0.91 strokes) @15:48:28.17 Air.in :default @15:48:28.25 Air.hold @15:48:28.51 Lysis1.in :extract @15:48:28.60 Lysis1.seek 800uL,nil @15:48:29.88 Air.in :glide @15:48:29.93 Air.seek :fullVent @15:48:33.06 Handoff.select :waste @15:48:33.19 Recovered 800uL lysate @15.1psi @15:48:33.20 delay 3 seconds @15:48:36.28 After 3 delay Recovered 800uL lysate @0.5psi @15:48:36.30 Plunge.seek :locked @15:48:37.63 Lysis2.in :DAmix @15:48:37.74 Lysis1.in :DAmix @15:48:38.33 Lysis2.seek 700uL @15:48:38.38 Lysis1.seek 550uL @15:49:13.34 delay 5 seconds @15:49:18.36 Handoff.select SPR @15:49:18.50 SPR.command "start 2 14" @15:49:19.17 SPR stabilized @15:49:19.31 delay 20 seconds @15:49:39.33 SPR.command "start 2 0" @15:49:42.51 Waiting up to 11:00 for SPR to enter state 1 @15:51:40.61 SPR.command "hold_on" @15:51:42.53 SPR Dilution 1 of 1 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:51:43.13 Lysis2.seek 400uL @15:51:43.17 Lysis1.seek 250uL @15:52:25.10 delay 15 seconds @15:52:40.12 SPR.command "hold_off" @15:52:42.66 Waiting up to 1:00:00 for SPR to enter state 7 @16:08:57.31 SPRsummary:40.5RIU,21.9RIU,35.8RIU,40.2RIU,high,high,high after 2563s @16:23:14.24 SPR analysis completed @16:23:18.80 Ended log spr26jul2015h.spr.bz2 @16:23:18.82 SPR.command "stats_off" @16:23:19.73 Handoff.select :close @16:23:19.88 Gate.power :heat,:OFF @16:23:19.90 Gate.power :cartridge,:OFF @16:23:19.98 PinPort.in :unlocking @16:23:20.05 PinPort.seek :clear @16:23:20.88 Carousel.in :default @16:23:21.00 Carousel.home.seek 36 @16:23:21.30 Plunge.seek :locked @16:23:21.46 Archive.in :unlocking @16:23:21.60 Air.in :unlocking @16:23:21.62 Archive.seek :clear @16:23:21.77 Air.seek :clear @16:23:21.89 Lysis1.in :unlocking @16:23:22.00 Lysis1.seek :clear @16:23:22.10 Lysis2.in :unlocking @16:23:22.16 Lysis2.seek :clear @16:23:26.56 Gate.power :analytic3,:OFF @16:23:26.58 Gate.power :motor,:OFF @16:23:26.61 Gate.power :analytic1,:OFF @16:23:26.63 Gate.power :analytic2,:OFF @16:23:26.66 Gate.power :core,:OFF @16:23:26.69 Gate.power :main,:OFF @16:23:26.71 Safely stopped and ready to power off @16:43:26.03 -> to 29 @16:43:26.08 BusOff in preston -- core bus was not powered while reading Plunge Valve (PV)'s status @16:43:30.06 -> esp :esp @16:43:35.33 -> ESP.ready! @16:43:35.37 Gate.power :main,:ON,limit to 5000mA @16:43:35.39 Gate.power :analytic1,:ON,limit to 4000mA @16:43:35.42 Gate.power :analytic2,:ON,limit to 4000mA @16:43:35.44 Gate.power :analytic3,:ON,limit to 4000mA @16:43:35.53 Powering core bus @16:43:38.24 Plunge.configure :default @16:43:38.33 SamplePump.configure :default @16:43:38.41 Toroid.configure :default @16:43:38.48 Carousel.configure :default @16:43:38.57 Abs.configure :default @16:43:38.65 PhaseSeparator.configure :default @16:43:38.73 Archive.configure :default @16:43:38.81 Air.configure :default @16:43:38.87 Lysis1.configure :default @16:43:38.93 Lysis2.configure :default @16:43:39.02 PinPort.configure :default @16:43:39.08 All elf controllers running firmware version 4.94 @16:43:39.10 Gate.power :motor,:ON,limit to 5000mA @16:43:42.78 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @16:43:42.80 AnalyticModule::Unknown in preston -- SPR @16:43:43.12 Plunge.home.jog 0 @16:43:43.21 Toroid.home.jog 0 @16:43:43.38 Carousel.home.jog 0 @16:43:43.46 Archive.home.jog 0 @16:43:43.56 Air.home.jog 0 @16:43:43.64 Lysis1.home.jog 0 @16:43:44.22 Lysis2.home.jog 0 @16:43:44.33 PinPort.home.jog 0 @16:43:45.00 Air.in :homing @16:43:45.05 Archive.in :homing @16:43:45.09 Toroid.in :homing @16:43:45.13 Lysis1.in :homing @16:43:45.16 Plunge.in :homing @16:43:45.33 Air.jog 1431 @16:43:45.40 Archive.jog 1626 @16:43:45.43 Lysis1.jog 1598 @16:43:45.46 Toroid.jog 1362 @16:43:45.49 Plunge.jog 1496 @16:43:45.81 PinPort.in :homing @16:43:45.83 Lysis2.in :homing @16:43:45.92 PinPort.jog 1230 @16:43:45.98 Lysis2.jog 1621 @16:43:46.71 Air.setRawPosition! 0 @16:43:46.77 Air.seek :home @16:43:46.87 Air.in :default @16:43:46.97 Lysis1.setRawPosition! 50 @16:43:47.00 Archive.setRawPosition! 50 @16:43:47.05 Plunge.setRawPosition! 50 @16:43:47.12 Lysis1.seek :home @16:43:47.18 Archive.seek :home @16:43:47.25 Plunge.seek :home @16:43:47.43 Lysis2.setRawPosition! 50 @16:43:47.49 Lysis2.seek :home @16:43:47.61 Lysis1.in :default @16:43:47.64 Archive.in :default @16:43:47.67 Plunge.in :default @16:43:47.85 Lysis2.in :default @16:43:47.87 PinPort.setRawPosition! 3150 @16:43:47.98 PinPort.seek :home @16:43:50.13 Toroid.setRawPosition! 0 @16:43:50.18 Toroid.seek :home @16:43:50.27 Toroid.in :default @16:43:52.35 PinPort.in :default @16:43:52.68 Toroid.seek :clear @16:43:52.82 PinPort.in :unlocking @16:43:52.88 PinPort.seek :clear @16:43:56.28 Air.seek :clear @16:43:56.37 Lysis1.seek :clear @16:43:57.09 Lysis2.seek :clear @16:43:57.89 Archive.seek :clear @16:43:58.67 Carousel.in :default @16:43:58.78 Carousel.home.seek 36 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 36 Abs = Absolute Pressure (Leak) Sensor @41psia PhaseSeparator = Intake Head Pressure @3.79psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @16:44:08.70 -> to 29 @16:44:08.95 Carousel.seek 29 @16:44:10.72 Carousel.reconfigure :align @16:44:10.83 Lysis1.in :fast @16:44:10.89 Lysis1.seek :locked @16:44:10.97 Slide::Warning in core -- Storage Carousel (SC) reseek at 29 + 4 counts (sensed 29 + 5 counts) @16:44:13.48 Plunge.seek :locked @16:44:13.62 Air.in :fast @16:44:13.69 Air.seek :locked @16:44:14.78 Carousel.reconfigure :locked @16:44:14.91 Powering cartridge bus @16:44:17.54 Gate.setIR 1750,5000,0,6 @16:44:17.66 Gate.setIR 0,5000,0,6 @16:44:17.75 Gate.power :cartridge,:OFF @16:44:17.79 Mounted archive Cartridge :archive_bac @16:44:23.96 -> Cartridge.unlock @16:44:24.04 Carousel.in :default @16:44:24.25 Carousel.home.seek 29 @16:44:24.61 Air.in :unlocking @16:44:24.74 Air.seek :clear @16:44:25.79 Lysis1.in :unlocking @16:44:25.92 Lysis1.seek :clear @16:44:25.96 Plunge.in :unlocking @16:44:26.07 Plunge.seek :clear @16:45:53.62 -> start {fire :lyseHold_phyto} @16:45:53.75 Cartridge::None in detached from preston -- No dry lyseHold_phyto cartridges remain # @16:46:00.01 -> slots {2 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul2008h.spr.bz2"}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [24,22] => { Type: :archiveHiBiomass_bac, State: :reserved, rank: 1, was: :dry}, [41,29] => { Type: :archive_bac, State: :missing}, 32 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 1000.0, lysed: 800, spr: spr26jul2015h.spr.bz2}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 42 => { Type: :lyseHold_phyto, State: :paused, filtered: 1000, rank: 1}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :paused}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @16:46:33.31 -> slot 42=>:dry {42 => { Type: :lyseHold_phyto, State: :dry, filtered: 1000, rank: 1}} @16:46:42.42 -> start {fire :lyseHold_phyto} # @16:46:43.12 Toroid.seek :clear @16:46:43.27 Air.in :default @16:46:43.32 Air.seek :clear @16:46:43.41 Lysis1.in :default @16:46:43.46 Lysis1.seek :clear @16:46:43.55 Lysis2.seek :clear @16:46:43.64 Archive.seek :clear @16:46:44.06 Carousel.in :default @16:46:44.25 Carousel.home.seek 29 @16:46:44.52 Selecting Cartridge 42 @16:46:44.77 Carousel.seek 42 @16:46:47.77 Carousel.reconfigure :align @16:46:47.89 Lysis1.in :fast @16:46:47.96 Lysis1.seek :locked @16:46:50.57 Plunge.in :default @16:46:50.62 Plunge.seek :locked @16:46:50.76 Air.in :fast @16:46:50.81 Air.seek :locked @16:46:51.89 Carousel.reconfigure :locked @16:46:52.03 Powering cartridge bus @16:46:54.66 Gate.setIR 1750,5000,0,6 @16:46:54.78 Gate.setIR 0,5000,0,6 @16:46:54.85 PuckHeater.configure :default #ambient 21.9C @16:46:54.92 Gate.power :heat,:ON,limit to 5000mA @16:46:55.07 Mounted lysis Cartridge board#J60008 @16:46:55.12 Using default cartridge heat loss curve for HeatingFunctionInCarousel @16:46:55.38 Preparing to filter lyseHold_phyto cartridge in slot 42 @16:46:55.40 Cartridge already filtered 1000.0ml @16:46:55.66 Cartridge::Error in detached from preston -- Cannot process dry cartridge 42 @16:46:55.68 Waiting to Resume from Checkpoint "Selecting Cartridge 42" @16:47:09.88 -> slot 42=>nil {42 => nil} @16:47:32.87 -> slot 42=>[:dry,:lyseHold_phyto] {42 => { Type: :lyseHold_phyto, State: :dry}} @16:48:35.08 -> Cart.unlock @16:48:35.12 Gate.power :heat,:OFF @16:48:35.14 Gate.power :cartridge,:OFF @16:48:35.22 Carousel.in :default @16:48:35.33 Carousel.home.seek 42 @16:48:35.70 Air.in :unlocking @16:48:35.80 Air.seek :clear @16:48:36.85 Lysis1.in :unlocking @16:48:36.98 Lysis1.seek :clear @16:48:37.03 Plunge.in :unlocking @16:48:37.13 Plunge.seek :clear @16:48:47.18 -> SC.to 28 @16:48:47.22 Carousel.seek 28 Storage Carousel (SC) at 28 @16:49:02.18 -> PP.to :cartridge @16:49:02.21 PinPort.in :default @16:49:02.28 PinPort.seek :cartridge Handoff Pin Port (PP) cartridge @16:49:13.42 -> Handoff.waste @16:49:13.44 Handoff.select :waste Handoff Valve::Manifold selects waste @16:49:38.08 -> Handoff.AM @16:49:38.10 Handoff.select :AM Handoff Valve::Manifold selects Analytical Module @16:52:08.16 -> PP.clear @16:52:08.18 PinPort.in :unlocking @16:52:08.25 PinPort.seek :clear Handoff Pin Port (PP) clear @16:52:11.71 -> SC.coast @16:52:11.73 Carousel.coast Storage Carousel (SC) at 28 @16:53:01.65 -> start {fire :lyseHold_phyto} @16:53:01.79 Selecting Cartridge 42 # @16:53:02.07 Carousel.seek 42 @16:53:04.24 Carousel.reconfigure :align @16:53:04.35 Lysis1.in :fast @16:53:04.41 Lysis1.seek :locked @16:53:07.03 Plunge.in :default @16:53:07.12 Plunge.seek :locked @16:53:07.24 Air.in :fast @16:53:07.31 Air.seek :locked @16:53:08.38 Carousel.reconfigure :locked @16:53:08.52 Powering cartridge bus @16:53:11.15 Gate.setIR 1750,5000,0,6 @16:53:11.27 Gate.setIR 0,5000,0,6 @16:53:11.34 PuckHeater.configure :default #ambient 21.9C @16:53:11.41 Gate.power :heat,:ON,limit to 5000mA @16:53:11.55 Mounted lysis Cartridge board#J60008 @16:53:11.60 Using default cartridge heat loss curve for HeatingFunctionInCarousel @16:53:11.86 Preparing to filter lyseHold_phyto cartridge in slot 42 @16:53:12.06 Air.in :default @16:53:12.17 Air.seek :sealed @16:53:12.26 Priming sample loop w/200ml, bypass w/10ml @16:53:12.27 SamplePump.setPosition! 0ml @16:53:12.34 Bypass.open @16:53:14.61 Intake.open @16:53:16.28 Sea 22.1m deep (47.4psia) @16:53:16.30 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:53:18.91 Vacuum tare is 64% of full scale at 22C (drifted 12%) @16:53:19.05 Intake.close @16:53:19.11 Bypass.close @16:53:19.18 Handoff.select :close @16:53:21.22 Verifying toroid holds at least 0.6..0.85 of 47.3psia @16:53:21.24 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @16:53:25.82 Bypass.open @16:53:27.36 Intake.open @16:53:29.72 Abs.in :default @16:53:29.78 Abs.hold @16:53:29.99 SamplePump.in :start @16:53:30.06 SamplePump.seek 10ml,1:10 @16:53:30.68 SamplePump.configure :prime @16:53:33.19 SamplePump.stop @16:53:33.26 Intake.close @16:53:33.33 Bypass.close @16:53:34.96 Abs.stop @16:53:35.17 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only -0.2psi vacuum after 2.1ml @16:53:35.19 Retry #1 of 2 @16:53:35.24 Selecting Cartridge 42 @16:53:35.74 Air.seek :sealed @16:53:35.90 Priming sample loop w/200ml, bypass w/10ml @16:53:35.92 SamplePump.setPosition! 0ml @16:53:35.98 Bypass.open @16:53:38.44 Bypass.close @16:53:40.01 Verifying toroid holds at least 0.6..0.85 of 47.6psia @16:53:40.05 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @16:53:44.64 Toroid pressure fell 1% to 47.2psia @16:53:44.65 Bypass.open @16:53:46.21 Intake.open @16:53:49.03 Abs.in :default @16:53:49.10 Abs.hold @16:53:49.33 SamplePump.in :start @16:53:49.41 SamplePump.seek 10ml,1:10 @16:53:50.02 SamplePump.configure :prime @16:53:52.55 SamplePump.stop @16:53:52.62 Intake.close @16:53:52.69 Bypass.close @16:53:54.32 Abs.stop @16:53:54.53 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only -0.1psi vacuum after 2.1ml @16:53:54.55 Retry #2 of 2 @16:53:54.60 Selecting Cartridge 42 @16:53:55.10 Air.seek :sealed @16:53:55.26 Priming sample loop w/200ml, bypass w/10ml @16:53:55.28 SamplePump.setPosition! 0ml @16:53:55.35 Bypass.open @16:53:57.78 Bypass.close @16:53:59.32 Verifying toroid holds at least 0.6..0.85 of 47.8psia @16:53:59.36 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @16:54:03.95 Toroid pressure fell 1% to 47.4psia @16:54:03.96 Bypass.open @16:54:05.50 Intake.open @16:54:07.87 Abs.in :default @16:54:07.94 Abs.hold @16:54:08.16 SamplePump.in :start @16:54:08.24 SamplePump.seek 10ml,1:10 @16:54:08.85 SamplePump.configure :prime @16:54:11.39 SamplePump.stop @16:54:11.46 Intake.close @16:54:11.53 Bypass.close @16:54:13.16 Abs.stop @16:54:13.87 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 0.76ml/s made only -0.1psi vacuum after 2.1ml @16:54:13.89 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 2.09ml -- with only 0.2 Volts!" @16:54:42.92 -> Leak.psia 47.6737848834518 @16:54:53.70 -> Sampler.psi -0.0964286875737344 @16:55:00.29 -> BACsampler.prime @16:55:00.38 Priming sample loop w/200ml, bypass w/10ml @16:55:00.40 SamplePump.setPosition! 0ml @16:55:00.45 Bypass.open @16:55:02.88 Bypass.close @16:55:04.41 Verifying toroid holds at least 0.6..0.85 of 47.3psia @16:55:04.43 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @16:55:09.02 Bypass.open @16:55:10.52 Intake.open @16:55:12.85 Abs.in :default @16:55:12.90 Abs.hold @16:55:13.10 SamplePump.in :start @16:55:13.17 SamplePump.seek 10ml,1:10 @16:55:13.79 SamplePump.configure :prime @16:55:16.30 SamplePump.stop @16:55:16.36 Intake.close @16:55:16.42 Bypass.close @16:55:18.10 Abs.stop @16:55:18.17 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.76ml/s made only -0.1psi vacuum after 2.1ml @16:55:33.97 -> 3.times{refillSampleLines} 3 @16:55:44.21 -> Sampler.refill @16:55:44.23 Bypass.open @16:55:45.77 Intake.open @16:55:47.37 SamplePump.setPosition! 0ml @16:55:47.48 SamplePump.in :default @16:55:47.55 SamplePump.seek 30ml @16:56:07.69 SamplePump.hold @16:56:07.87 delay 1 second @16:56:08.90 Intake.close @16:56:08.95 Bypass.close @16:56:10.64 UserAbort in preston -- Sample Pump (SP) seek to 30ml Aborted! @17:03:56.33 -> PB.open;Intake.open @17:03:56.35 Bypass.open @17:03:57.86 Intake.open Intake is opened @17:04:03.94 -> SP.volume=0 @17:04:03.97 SamplePump.setPosition! 0ml 0 @17:04:28.77 -> SP.pump 100 @17:04:28.85 SamplePump.seek 100ml @17:04:31.46 SamplePump.hold @17:04:32.16 UserAbort in preston -- Sample Pump (SP) seek to 100ml Aborted! @17:05:26.15 -> BACsampler.prime @17:05:26.24 Priming sample loop w/200ml, bypass w/10ml @17:05:26.25 SamplePump.setPosition! 0ml @17:05:27.20 Intake.close @17:05:27.25 Bypass.close @17:05:28.91 Verifying toroid holds at least 0.6..0.85 of 47.4psia @17:05:28.94 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:05:33.52 Toroid pressure rose 1% to 47.8psia @17:05:33.53 Bypass.open @17:05:35.08 Intake.open @17:05:37.43 Abs.in :default @17:05:37.49 Abs.hold @17:05:37.69 SamplePump.in :start @17:05:37.76 SamplePump.seek 10ml,1:10 @17:05:38.39 SamplePump.configure :prime @17:05:40.90 SamplePump.stop @17:05:40.97 Intake.close @17:05:41.04 Bypass.close @17:05:42.69 Abs.stop @17:05:42.75 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.76ml/s made only -0.1psi vacuum after 2.1ml @17:07:09.05 -> BACsampler.prime @17:07:09.14 Priming sample loop w/200ml, bypass w/10ml @17:07:09.15 SamplePump.setPosition! 0ml @17:07:09.22 Bypass.open @17:07:11.66 Bypass.close @17:07:13.21 Verifying toroid holds at least 0.6..0.85 of 47.3psia @17:07:13.24 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:07:17.83 Bypass.open @17:07:19.34 Intake.open @17:07:21.72 Abs.in :default @17:07:21.78 Abs.hold @17:07:22.56 SamplePump.in :start @17:07:22.63 SamplePump.seek 10ml,1:10 @17:07:23.25 SamplePump.configure :prime @17:07:25.77 SamplePump.stop @17:07:25.83 Intake.close @17:07:25.91 Bypass.close @17:07:27.52 Abs.stop @17:07:27.59 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.76ml/s made only -0.4psi vacuum after 2.1ml @17:09:19.05 -> BACsampler.prime @17:09:19.14 Priming sample loop w/200ml, bypass w/10ml @17:09:19.16 SamplePump.setPosition! 0ml @17:09:19.22 Bypass.open @17:09:21.63 Bypass.close @17:09:23.15 Verifying toroid holds at least 0.6..0.85 of 47.6psia @17:09:23.17 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:09:27.75 Toroid pressure fell 1% to 47.1psia @17:09:27.76 Bypass.open @17:09:29.28 Intake.open @17:09:31.62 Abs.in :default @17:09:31.68 Abs.hold @17:09:31.89 SamplePump.in :start @17:09:31.96 SamplePump.seek 10ml,1:10 @17:09:32.58 SamplePump.configure :prime @17:09:35.10 SamplePump.stop @17:09:35.17 Intake.close @17:09:35.21 Bypass.close @17:09:36.90 Abs.stop @17:09:36.97 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.76ml/s made only -0.3psi vacuum after 2.1ml @17:11:04.43 -> ESP.shutdown @17:11:04.59 Gate.power :heat,:OFF @17:11:04.60 Gate.power :cartridge,:OFF @17:11:04.63 Gate.power :analytic3,:OFF @17:11:04.67 Gate.power :motor,:OFF @17:11:04.69 Gate.power :analytic1,:OFF @17:11:04.71 Gate.power :analytic2,:OFF @17:11:04.74 Gate.power :core,:OFF @17:11:04.77 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @17:13:43.77 -> ESP.reready! @17:13:43.81 Gate.power :main,:ON,limit to 5000mA @17:13:43.83 Gate.power :analytic1,:ON,limit to 4000mA @17:13:43.85 Gate.power :analytic2,:ON,limit to 4000mA @17:13:43.88 Gate.power :analytic3,:ON,limit to 4000mA @17:13:43.96 Powering core bus @17:13:46.67 Plunge.configure :default @17:13:46.75 SamplePump.configure :default @17:13:46.84 Toroid.configure :default @17:13:46.91 Carousel.configure :locked @17:13:46.99 Abs.configure :default @17:13:47.08 PhaseSeparator.configure :default @17:13:47.16 Archive.configure :default @17:13:48.09 Air.configure :default @17:13:48.14 Lysis1.configure :default @17:13:48.21 Lysis2.configure :default @17:13:48.28 PinPort.configure :default @17:13:48.34 All elf controllers running firmware version 4.94 @17:13:48.36 Gate.power :motor,:ON,limit to 5000mA @17:13:51.28 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @17:13:51.31 AnalyticModule::Unknown in preston -- SPR @17:13:51.36 Plunge.home.jog 0 @17:13:51.41 Toroid.home.jog 0 @17:13:51.47 Carousel.in :default @17:13:51.56 Archive.home.jog 0 @17:13:51.67 Air.home.jog 0 @17:13:51.76 Lysis1.home.jog 0 @17:13:51.80 Lysis2.home.jog 0 @17:13:51.91 PinPort.home.jog 0 @17:13:52.04 Carousel.home.jog 0 @17:13:52.62 Toroid.in :homing @17:13:52.66 Plunge.in :homing @17:13:52.84 Toroid.jog 1365 @17:13:52.87 Plunge.jog 1486 @17:13:53.07 Lysis2.in :homing @17:13:53.11 Air.in :homing @17:13:53.14 Archive.in :homing @17:13:53.17 PinPort.in :homing @17:13:53.20 Lysis1.in :homing @17:13:53.36 Lysis2.jog 1618 @17:13:53.44 PinPort.jog 1224 @17:13:53.46 Air.jog 2471 @17:13:53.50 Archive.jog 301 @17:13:53.53 Lysis1.jog 2264 @17:13:54.18 Plunge.setRawPosition! 50 @17:13:54.26 Plunge.seek :home @17:13:54.28 Archive.setRawPosition! 50 @17:13:54.39 Archive.seek :home @17:13:54.58 Plunge.in :default @17:13:54.67 Archive.in :default @17:13:54.84 Lysis2.setRawPosition! 50 @17:13:54.90 Lysis2.seek :home @17:13:55.18 Lysis2.in :default @17:13:55.27 Lysis1.setRawPosition! 50 @17:13:55.34 Air.setRawPosition! 0 @17:13:55.37 Lysis1.seek :home @17:13:55.48 Air.seek :home @17:13:55.59 PinPort.setRawPosition! 3150 @17:13:55.61 Air.in :default @17:13:55.70 PinPort.seek :home @17:13:55.81 Lysis1.in :default @17:13:57.41 Toroid.setRawPosition! 0 @17:13:57.47 Toroid.seek :home @17:13:57.56 Toroid.in :default @17:14:00.09 PinPort.in :default @17:14:00.42 Toroid.seek :clear @17:14:00.55 PinPort.in :unlocking @17:14:00.62 PinPort.seek :clear @17:14:04.06 Air.seek :clear @17:14:04.14 Lysis1.seek :clear @17:14:05.43 Lysis2.seek :clear @17:14:06.24 Archive.seek :clear @17:14:07.03 Carousel.in :default @17:14:07.15 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @23psia PhaseSeparator = Intake Head Pressure @3.86psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:14:11.35 -> BACsampler.prime @17:14:11.44 Priming sample loop w/200ml, bypass w/10ml @17:14:11.45 SamplePump.setPosition! 0ml @17:14:11.52 Bypass.open @17:14:13.94 Bypass.close @17:14:15.46 Verifying toroid holds at least 0.6..0.85 of 47.1psia @17:14:15.48 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:14:20.06 Toroid pressure fell 1% to 46.5psia @17:14:20.07 Bypass.open @17:14:21.60 Intake.open @17:14:23.95 Abs.in :default @17:14:24.01 Abs.hold @17:14:24.23 SamplePump.in :start @17:14:24.30 SamplePump.seek 10ml,1:10 @17:14:24.93 SamplePump.configure :prime @17:14:27.06 SamplePump.hold @17:14:27.30 Intake.close @17:14:27.37 Bypass.close @17:14:28.99 Abs.stop @17:14:29.07 UserAbort in preston -- Sample Pump (SP) seek to 10ml Aborted! @17:14:38.20 -> ESP.shutdown @17:14:38.36 Gate.power :analytic3,:OFF @17:14:38.38 Gate.power :motor,:OFF @17:14:38.40 Gate.power :analytic1,:OFF @17:14:38.43 Gate.power :analytic2,:OFF @17:14:38.45 Gate.power :core,:OFF @17:14:38.48 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @17:16:52.74 -> ESP.ready! @17:16:52.78 Gate.power :main,:ON,limit to 5000mA @17:16:52.80 Gate.power :analytic1,:ON,limit to 4000mA @17:16:52.82 Gate.power :analytic2,:ON,limit to 4000mA @17:16:52.85 Gate.power :analytic3,:ON,limit to 4000mA @17:16:52.93 Powering core bus @17:16:55.64 Plunge.configure :default @17:16:56.33 SamplePump.configure :default @17:16:56.42 Toroid.configure :default @17:16:56.49 Carousel.configure :default @17:16:56.58 Abs.configure :default @17:16:56.66 PhaseSeparator.configure :default @17:16:56.99 Archive.configure :default @17:16:57.07 Air.configure :default @17:16:57.12 Lysis1.configure :default @17:16:57.19 Lysis2.configure :default @17:16:57.26 PinPort.configure :default @17:16:57.32 All elf controllers running firmware version 4.94 @17:16:57.35 Gate.power :motor,:ON,limit to 5000mA @17:17:00.20 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @17:17:00.22 AnalyticModule::Unknown in preston -- SPR @17:17:00.47 Plunge.home.jog 0 @17:17:01.00 Lysis2.home.jog 0 @17:17:01.04 Lysis1.home.jog 0 @17:17:01.07 Air.home.jog 0 @17:17:01.10 Archive.home.jog 0 @17:17:01.13 Toroid.home.jog 0 @17:17:01.17 PinPort.home.jog 0 @17:17:01.20 Carousel.home.jog 0 @17:17:01.81 Plunge.in :homing @17:17:01.99 Plunge.jog 443 @17:17:02.16 Lysis2.in :homing @17:17:02.22 Air.in :homing @17:17:02.25 Archive.in :homing @17:17:02.28 PinPort.in :homing @17:17:02.31 Lysis1.in :homing @17:17:02.34 Toroid.in :homing @17:17:02.54 Lysis2.jog 1621 @17:17:02.57 Air.jog 1429 @17:17:02.63 PinPort.jog 1215 @17:17:02.67 Archive.jog 1627 @17:17:02.71 Lysis1.jog 1598 @17:17:02.81 Toroid.jog 1365 @17:17:03.04 Plunge.setRawPosition! 50 @17:17:03.10 Plunge.seek :home @17:17:03.38 Plunge.in :default @17:17:03.97 Air.setRawPosition! 0 @17:17:04.06 Lysis2.setRawPosition! 50 @17:17:04.07 Air.seek :home @17:17:04.19 Lysis2.seek :home @17:17:04.32 Lysis1.setRawPosition! 50 @17:17:04.34 Air.in :default @17:17:04.37 Archive.setRawPosition! 50 @17:17:04.49 Lysis1.seek :home @17:17:04.58 Archive.seek :home @17:17:04.69 PinPort.setRawPosition! 3150 @17:17:04.75 Lysis2.in :default @17:17:04.81 PinPort.seek :home @17:17:04.94 Lysis1.in :default @17:17:04.96 Archive.in :default @17:17:07.48 Toroid.setRawPosition! 0 @17:17:07.54 Toroid.seek :home @17:17:07.61 Toroid.in :default @17:17:09.21 PinPort.in :default @17:17:10.16 Toroid.seek :clear @17:17:10.29 PinPort.in :unlocking @17:17:10.36 PinPort.seek :clear @17:17:13.79 Air.seek :clear @17:17:13.88 Lysis1.seek :clear @17:17:14.61 Lysis2.seek :clear @17:17:15.39 Archive.seek :clear @17:17:16.18 Carousel.in :default @17:17:16.30 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @27psia PhaseSeparator = Intake Head Pressure @3.84psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:17:18.00 -> Sampler.psi -0.325862461456012 @17:17:48.78 -> PB.open;Intake.open @17:17:48.80 Bypass.open @17:17:50.35 Intake.open Intake is opened @17:17:58.71 -> SP.pump 100 @17:17:58.80 Slide::Error in preston -- Sample Pump (SP) Not yet Homed! @17:18:05.10 -> SP.volume=0 @17:18:05.12 SamplePump.setPosition! 0ml 0 @17:18:10.03 -> SP.pump 100,:slow @17:18:10.11 SamplePump.in :slow @17:18:10.18 SamplePump.seek 100ml @17:18:12.93 SamplePump.hold @17:18:13.10 UserAbort in preston -- Sample Pump (SP) seek to 100ml Aborted! @17:21:26.39 -> SP.pump 100,:slow @17:21:26.47 SamplePump.seek 101.01ml @17:21:32.11 SamplePump.hold @17:21:32.29 UserAbort in preston -- Sample Pump (SP) seek to 101.01ml Aborted! @17:22:44.58 -> BACsampler.prime @17:22:44.68 Priming sample loop w/200ml, bypass w/10ml @17:22:44.69 SamplePump.setPosition! 0ml @17:22:45.64 Intake.close @17:22:45.70 Bypass.close @17:22:47.37 Verifying toroid holds at least 0.6..0.85 of 47.5psia @17:22:47.39 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:22:51.97 Bypass.open @17:22:53.53 Intake.open @17:22:56.54 Abs.in :default @17:22:56.62 Abs.hold @17:22:56.83 SamplePump.in :start @17:22:56.90 SamplePump.seek 10ml,1:10 @17:22:57.52 SamplePump.configure :prime @17:23:00.04 SamplePump.stop @17:23:00.10 Intake.close @17:23:00.14 Bypass.close @17:23:01.85 Abs.stop @17:23:01.91 Cartridge::Sampler::Leaky in preston -- Serious Leak or Stall -- 0.76ml/s made only 0.1psi vacuum after 2.1ml @17:24:41.02 -> ESP.shutdown @17:24:41.18 Gate.power :analytic3,:OFF @17:24:41.20 Gate.power :motor,:OFF @17:24:41.22 Gate.power :analytic1,:OFF @17:24:41.25 Gate.power :analytic2,:OFF @17:24:41.27 Gate.power :core,:OFF @17:24:41.29 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @17:25:48.94 -> ESP.ready1 @17:25:48.96 NoMethodError in preston -- undefined method `ready1' for ESP:Module @17:25:52.92 -> ESP.ready1 @17:25:52.94 NoMethodError in preston -- undefined method `ready1' for ESP:Module @17:25:55.72 -> ESP.ready! @17:25:55.77 Gate.power :main,:ON,limit to 5000mA @17:25:55.78 Gate.power :analytic1,:ON,limit to 4000mA @17:25:55.80 Gate.power :analytic2,:ON,limit to 4000mA @17:25:55.83 Gate.power :analytic3,:ON,limit to 4000mA @17:25:55.91 Powering core bus @17:25:58.62 Plunge.configure :default @17:25:58.69 SamplePump.configure :default @17:25:58.78 Toroid.configure :default @17:25:58.85 Carousel.configure :default @17:25:58.93 Abs.configure :default @17:25:59.02 PhaseSeparator.configure :default @17:25:59.10 Archive.configure :default @17:25:59.17 Air.configure :default @17:25:59.23 Lysis1.configure :default @17:25:59.30 Lysis2.configure :default @17:25:59.38 PinPort.configure :default @17:25:59.44 WARNING: Multiple controller firmware versions in use [:sampler0, :sampler1]=>4.92, [:carousel1, :syringe0, :syringe1, :valve0, :ambient0, :valve1, :carousel0]=>4.94 @17:25:59.46 Gate.power :motor,:ON,limit to 5000mA @17:26:03.17 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @17:26:03.19 AnalyticModule::Unknown in preston -- SPR @17:26:03.40 Plunge.home.jog 0 @17:26:03.93 PinPort.home.jog 0 @17:26:03.96 Lysis2.home.jog 0 @17:26:03.99 Lysis1.home.jog 0 @17:26:04.04 Air.home.jog 0 @17:26:04.07 Carousel.home.jog 0 @17:26:04.12 Toroid.home.jog 0 @17:26:04.14 Archive.home.jog 0 @17:26:04.72 Plunge.in :homing @17:26:04.97 Plunge.jog 435 @17:26:05.16 PinPort.in :homing @17:26:05.20 Lysis2.in :homing @17:26:05.23 Lysis1.in :homing @17:26:05.26 Archive.in :homing @17:26:05.29 Toroid.in :homing @17:26:05.32 Air.in :homing @17:26:05.53 PinPort.jog 1212 @17:26:06.21 Lysis2.jog 1621 @17:26:06.27 Air.jog 1431 @17:26:06.31 Toroid.jog 1368 @17:26:06.33 Lysis1.jog 1598 @17:26:06.36 Archive.jog 300 @17:26:06.70 Plunge.setRawPosition! 50 @17:26:06.75 Plunge.seek :home @17:26:07.02 Plunge.in :default @17:26:07.12 Archive.setRawPosition! 50 @17:26:07.17 Archive.seek :home @17:26:07.45 Archive.in :default @17:26:07.72 Lysis2.setRawPosition! 50 @17:26:07.74 Air.setRawPosition! 0 @17:26:07.85 Air.seek :home @17:26:07.87 Lysis2.seek :home @17:26:08.05 Air.in :default @17:26:08.08 Lysis1.setRawPosition! 50 @17:26:08.17 Lysis1.seek :home @17:26:08.30 PinPort.setRawPosition! 3150 @17:26:08.31 Lysis2.in :default @17:26:08.39 PinPort.seek :home @17:26:08.52 Lysis1.in :default @17:26:11.09 Toroid.setRawPosition! 0 @17:26:11.14 Toroid.seek :home @17:26:11.23 Toroid.in :default @17:26:12.80 PinPort.in :default @17:26:13.13 Toroid.seek :clear @17:26:13.27 PinPort.in :unlocking @17:26:13.33 PinPort.seek :clear @17:26:16.78 Air.seek :clear @17:26:16.87 Lysis1.seek :clear @17:26:17.60 Lysis2.seek :clear @17:26:18.39 Archive.seek :clear @17:26:19.18 Carousel.in :default @17:26:19.29 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @26psia PhaseSeparator = Intake Head Pressure @3.88psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:26:26.04 -> PB.open;Intake.open @17:26:26.06 Bypass.open @17:26:27.57 Intake.open Intake is opened @17:26:35.29 -> SP.pump 100 @17:26:35.37 Slide::Error in preston -- Sample Pump (SP) Not yet Homed! @17:26:39.85 -> SP.volumt=0 @17:26:39.87 NoMethodError in preston -- undefined method `volumt=' for SamplePump:Pump @17:26:45.79 -> SP.volume=0 @17:26:45.81 SamplePump.setPosition! 0ml 0 @17:26:49.96 -> SP.pump 100,:slow @17:26:50.04 SamplePump.in :slow @17:26:50.11 SamplePump.seek 100ml @17:27:00.77 SamplePump.hold @17:27:00.93 UserAbort in preston -- Sample Pump (SP) seek to 100ml Aborted! @17:27:03.86 -> ESP.shutdown @17:27:03.96 Intake.close @17:27:04.00 Bypass.close @17:27:05.78 Gate.power :analytic3,:OFF @17:27:05.80 Gate.power :motor,:OFF @17:27:05.83 Gate.power :analytic1,:OFF @17:27:05.86 Gate.power :analytic2,:OFF @17:27:05.88 Gate.power :core,:OFF @17:27:05.91 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @17:27:39.21 -> ESP.ready! @17:27:39.26 Gate.power :main,:ON,limit to 5000mA @17:27:39.27 Gate.power :analytic1,:ON,limit to 4000mA @17:27:39.30 Gate.power :analytic2,:ON,limit to 4000mA @17:27:39.33 Gate.power :analytic3,:ON,limit to 4000mA @17:27:39.40 Powering core bus @17:27:42.11 Plunge.configure :default @17:27:42.19 SamplePump.configure :default @17:27:42.26 Toroid.configure :default @17:27:42.35 Carousel.configure :default @17:27:42.42 Abs.configure :default @17:27:42.51 PhaseSeparator.configure :default @17:27:42.58 Archive.configure :default @17:27:42.66 Air.configure :default @17:27:42.71 Lysis1.configure :default @17:27:42.78 Lysis2.configure :default @17:27:42.86 PinPort.configure :default @17:27:42.91 WARNING: Multiple controller firmware versions in use [:sampler0, :sampler1]=>4.92, [:carousel1, :syringe0, :syringe1, :valve0, :ambient0, :valve1, :carousel0]=>4.94 @17:27:42.94 Gate.power :motor,:ON,limit to 5000mA @17:27:46.66 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @17:27:46.68 AnalyticModule::Unknown in preston -- SPR @17:27:46.86 Plunge.home.jog 0 @17:27:47.43 Lysis2.home.jog 0 @17:27:47.48 Lysis1.home.jog 0 @17:27:47.51 Air.home.jog 0 @17:27:47.53 Archive.home.jog 0 @17:27:47.58 Toroid.home.jog 0 @17:27:47.61 PinPort.home.jog 0 @17:27:47.64 Carousel.home.jog 0 @17:27:48.29 Plunge.in :homing @17:27:48.51 Plunge.jog 435 @17:27:48.57 Lysis2.in :homing @17:27:48.62 Lysis1.in :homing @17:27:48.65 Archive.in :homing @17:27:48.68 Air.in :homing @17:27:48.70 Toroid.in :homing @17:27:48.74 PinPort.in :homing @17:27:48.97 Lysis1.jog 1598 @17:27:49.04 Lysis2.jog 1619 @17:27:49.08 Archive.jog 1625 @17:27:49.12 Air.jog 1430 @17:27:49.16 Toroid.jog 1362 @17:27:49.19 PinPort.jog 1213 @17:27:49.51 Plunge.setRawPosition! 50 @17:27:49.56 Plunge.seek :home @17:27:49.84 Plunge.in :default @17:27:50.52 Lysis2.setRawPosition! 50 @17:27:50.55 Lysis1.setRawPosition! 50 @17:27:50.66 Lysis1.seek :home @17:27:50.68 Lysis2.seek :home @17:27:50.84 Archive.setRawPosition! 50 @17:27:50.87 Air.setRawPosition! 0 @17:27:50.96 Archive.seek :home @17:27:51.63 Air.seek :home @17:27:51.83 PinPort.setRawPosition! 3150 @17:27:51.87 Lysis2.in :default @17:27:51.89 Air.in :default @17:27:51.92 Lysis1.in :default @17:27:52.05 PinPort.seek :home @17:27:52.20 Archive.in :default @17:27:53.89 Toroid.setRawPosition! 0 @17:27:53.95 Toroid.seek :home @17:27:54.03 Toroid.in :default @17:27:56.48 PinPort.in :default @17:27:56.82 Toroid.seek :clear @17:27:56.95 PinPort.in :unlocking @17:27:57.03 PinPort.seek :clear @17:28:00.48 Air.seek :clear @17:28:00.57 Lysis1.seek :clear @17:28:01.29 Lysis2.seek :clear @17:28:02.07 Archive.seek :clear @17:28:02.86 Carousel.in :default @17:28:02.97 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @22psia PhaseSeparator = Intake Head Pressure @3.71psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:28:08.95 -> PB.open;Intake.open @17:28:08.97 Bypass.open @17:28:10.53 Intake.open Intake is opened @17:28:22.66 -> SP.pump 100,:slow @17:28:22.74 Slide::Error in preston -- Sample Pump (SP) Not yet Homed! @17:28:28.80 -> SP.volume=0 @17:28:28.82 SamplePump.setPosition! 0ml 0 @17:28:31.64 -> SP.pump 100,:slow @17:28:31.72 SamplePump.in :slow @17:28:31.78 SamplePump.seek 100ml @17:28:32.88 SamplePump.hold @17:28:33.04 UserAbort in preston -- Sample Pump (SP) seek to 100ml Aborted! @17:29:25.72 -> ESP.shutdown @17:29:25.81 Intake.close @17:29:25.86 Bypass.close @17:29:27.59 Gate.power :analytic3,:OFF @17:29:27.60 Gate.power :motor,:OFF @17:29:27.63 Gate.power :analytic1,:OFF @17:29:27.66 Gate.power :analytic2,:OFF @17:29:27.69 Gate.power :core,:OFF @17:29:27.70 Gate.power :main,:OFF [:analytic3,:motor,:analytic1,:analytic2,:core,:main] @17:31:02.31 -> ESP.ready! @17:31:02.36 Gate.power :main,:ON,limit to 5000mA @17:31:02.38 Gate.power :analytic1,:ON,limit to 4000mA @17:31:02.40 Gate.power :analytic2,:ON,limit to 4000mA @17:31:02.43 Gate.power :analytic3,:ON,limit to 4000mA @17:31:02.51 Powering core bus @17:31:05.22 Plunge.configure :default @17:31:05.29 SamplePump.configure :default @17:31:05.38 Toroid.configure :default @17:31:05.45 Carousel.configure :default @17:31:05.52 Abs.configure :default @17:31:05.60 PhaseSeparator.configure :default @17:31:06.59 Archive.configure :default @17:31:06.68 Air.configure :default @17:31:06.73 Lysis1.configure :default @17:31:06.80 Lysis2.configure :default @17:31:06.87 PinPort.configure :default @17:31:06.93 All elf controllers running firmware version 4.94 @17:31:06.96 Gate.power :motor,:ON,limit to 5000mA @17:31:09.78 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @17:31:09.80 AnalyticModule::Unknown in preston -- SPR @17:31:09.98 Plunge.home.jog 0 @17:31:10.55 PinPort.home.jog 0 @17:31:10.58 Lysis2.home.jog 0 @17:31:10.60 Lysis1.home.jog 0 @17:31:10.64 Air.home.jog 0 @17:31:10.67 Archive.home.jog 0 @17:31:10.70 Carousel.home.jog 0 @17:31:10.72 Toroid.home.jog 0 @17:31:11.30 Plunge.in :homing @17:31:11.63 Plunge.jog 435 @17:31:11.68 PinPort.in :homing @17:31:11.71 Lysis1.in :homing @17:31:11.74 Air.in :homing @17:31:11.77 Toroid.in :homing @17:31:11.81 Lysis2.in :homing @17:31:11.84 Archive.in :homing @17:31:12.05 PinPort.jog 1213 @17:31:12.14 Lysis1.jog 1598 @17:31:12.18 Air.jog 1428 @17:31:12.21 Archive.jog 1626 @17:31:12.24 Lysis2.jog 1621 @17:31:12.27 Toroid.jog 1365 @17:31:12.61 Plunge.setRawPosition! 50 @17:31:12.67 Plunge.seek :home @17:31:12.96 Plunge.in :default @17:31:13.57 Air.setRawPosition! 0 @17:31:13.64 Air.seek :home @17:31:13.76 Lysis1.setRawPosition! 50 @17:31:13.80 Archive.setRawPosition! 50 @17:31:13.83 Lysis2.setRawPosition! 50 @17:31:13.85 Air.in :default @17:31:13.99 Lysis1.seek :home @17:31:14.03 Archive.seek :home @17:31:14.13 Lysis2.seek :home @17:31:14.33 PinPort.setRawPosition! 3150 @17:31:14.39 PinPort.seek :home @17:31:14.53 Lysis2.in :default @17:31:14.55 Lysis1.in :default @17:31:14.58 Archive.in :default @17:31:16.98 Toroid.setRawPosition! 0 @17:31:17.04 Toroid.seek :home @17:31:17.11 Toroid.in :default @17:31:18.77 PinPort.in :default @17:31:19.10 Toroid.seek :clear @17:31:19.23 PinPort.in :unlocking @17:31:19.28 PinPort.seek :clear @17:31:22.71 Air.seek :clear @17:31:22.87 Lysis1.seek :clear @17:31:23.59 Lysis2.seek :clear @17:31:24.37 Archive.seek :clear @17:31:25.16 Carousel.in :default @17:31:25.28 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @25psia PhaseSeparator = Intake Head Pressure @3.70psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @17:31:26.83 -> Intake.open @17:31:26.86 Intake.open Intake is opened @17:32:02.90 -> Intake.close @17:32:02.92 Intake.close Intake is closed @17:32:30.67 -> Intake.open @17:32:30.70 Intake.open Intake is opened @17:33:07.70 -> Intake.close @17:33:07.72 Intake.close Intake is closed @17:33:55.66 -> Intake.close Intake is closed @17:33:59.50 -> Intake.close Intake is closed @17:34:05.01 -> Intake.open @17:34:05.03 Intake.open Intake is opened @17:34:14.11 -> Intake.close @17:34:14.13 Intake.close Intake is closed @17:34:41.94 -> Intake.open @17:34:41.96 Intake.open Intake is opened @17:34:50.00 -> Intake.close @17:34:50.02 Intake.close Intake is closed @17:34:57.09 -> Intake.open @17:34:57.11 Intake.open Intake is opened @17:35:03.07 -> Intake.close @17:35:03.09 Intake.close Intake is closed @17:35:58.30 -> Intake.open @17:35:58.31 Intake.open Intake is opened @17:36:15.19 -> Intake.close @17:36:15.22 Intake.close Intake is closed @17:36:44.16 -> slots {2 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul2008h.spr.bz2"}, 4 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr26jul1919h.spr.bz2"}, 16 => { Type: :archiveHiBiomass_bac, State: :reserved, was: :dry}, [24,22] => { Type: :archiveHiBiomass_bac, State: :reserved, rank: 1, was: :dry}, [41,29] => { Type: :archive_bac, State: :missing}, 32 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000.0, rank: 1}, 34 => { Type: :archiveHiBiomass_bac, State: :processed, filtered: 1000}, 36 => { Type: :microSPR_phyto, State: :processed, filtered: 1000.0, lysed: 800, spr: spr26jul2015h.spr.bz2}, 40 => { Type: :lyseHold_phyto, State: :processed, filtered: 1000, lysed: 800}, 42 => { Type: :lyseHold_phyto, State: :dry, rank: 3}, 48 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1909h.spr.bz2"}, 50 => { Type: :microSPR_phyto, State: :paused}, 52 => { Type: :microSPR_phyto, State: :processed, filtered: 100, lysed: 800, spr: "spr26jul1917h.spr.bz2"}, 54 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1818h.spr.bz2"}, 56 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1816h.spr.bz2"}, 58 => { Type: :microSPR_phyto, State: :processed, filtered: 1000, lysed: 800, spr: "spr26jul1815h.spr.bz2"}, 60 => { Type: :microSPR_phyto, State: :analyzing, filtered: 1000, lysed: 800, spr: "spr26jul1812h.spr.bz2"}} @17:36:59.56 -> start {fire :lyseHold_phyto} @17:36:59.72 Selecting Cartridge 42 # @17:36:59.99 Carousel.seek 42 @17:37:00.25 Carousel.reconfigure :align @17:37:00.36 Lysis1.in :fast @17:37:00.43 Lysis1.seek :locked @17:37:03.03 Plunge.seek :locked @17:37:03.17 Air.in :fast @17:37:03.24 Air.seek :locked @17:37:04.31 Carousel.reconfigure :locked @17:37:04.45 Powering cartridge bus @17:37:07.64 Gate.setIR 1750,5000,0,6 @17:37:07.83 Gate.setIR 0,5000,0,6 @17:37:07.90 PuckHeater.configure :default #ambient 21.9C @17:37:07.97 Gate.power :heat,:ON,limit to 5000mA @17:37:08.12 Mounted lysis Cartridge board#J60008 @17:37:08.17 Using default cartridge heat loss curve for HeatingFunctionInCarousel @17:37:08.46 Preparing to filter lyseHold_phyto cartridge in slot 42 @17:37:08.49 Cmd::Error in detached from preston#2 -- Cannot process while in READY state @17:37:08.55 Waiting to Resume from Checkpoint "Selecting Cartridge 42" @17:37:08.73 Air.in :default @17:37:08.85 Air.seek :sealed @17:37:08.88 Priming sample loop w/200ml, bypass w/10ml @17:37:08.93 SamplePump.setPosition! 0ml @17:37:09.01 Bypass.open @17:37:11.40 Bypass.close @17:37:12.94 Verifying toroid holds at least 0.6..0.85 of 47.4psia @17:37:12.97 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:37:17.56 Toroid pressure fell 1% to 46.8psia @17:37:17.57 Bypass.open @17:37:19.13 Intake.open @17:37:21.53 Abs.in :default @17:37:21.60 Abs.hold @17:37:21.81 SamplePump.in :start @17:37:21.87 SamplePump.seek 10ml,1:10 @17:37:22.50 SamplePump.configure :prime @17:37:35.39 Abs.stop @17:37:35.45 SamplePump.setPosition! 0ml @17:37:35.51 Exhaust.open @17:37:37.71 Bypass.close @17:37:40.04 Abs.in :default @17:37:40.10 Abs.hold @17:37:40.23 Sea 22.2m deep (47.5psia) @17:37:40.40 SamplePump.in :start @17:37:40.48 SamplePump.seek 200ml,13:50 @17:37:41.09 SamplePump.configure :prime @17:42:03.79 Abs.stop @17:42:03.95 Bypass.open @17:42:05.48 Intake.close @17:42:07.17 Toroid.in :hold @17:42:07.24 Toroid.seek :crack @17:42:09.71 Toroid.seek :cartridge @17:42:11.84 Toroid.in :default @17:42:11.90 Toroid.seek :clear @17:42:15.84 Exhaust.close @17:42:15.87 Bypass.close @17:42:17.44 Verifying cartridge holds at least 0.65..0.85 of 47.5psia @17:42:17.46 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @17:42:22.09 Sampling 1000.0ml @17:42:22.11 SamplePump.setPosition! 0ml @17:42:22.18 Exhaust.open @17:42:23.65 Intake.open @17:42:25.39 Abs.in :default @17:42:25.46 Abs.hold @17:42:25.58 Sea 22.3m deep (47.6psia) @17:42:25.75 SamplePump.in :start @17:42:25.82 SamplePump.seek 1000ml,56:04 @17:42:26.44 SamplePump.configure :sample @17:42:28.83 Cartridge 42 filtering 2/1000ml at 0.61ml/s with 7.7psi vacuum 24.0m deep (50.2psia) @17:43:28.88 Cartridge 42 filtering 40/1000ml at 0.64ml/s with 10.2psi vacuum 27.3m deep (55.1psia) @17:44:28.88 Cartridge 42 filtering 79/1000ml at 0.64ml/s with 13.8psi vacuum 18.2m deep (41.7psia) @17:45:28.88 Cartridge 42 filtering 117/1000ml at 0.64ml/s with 13.8psi vacuum 20.7m deep (45.3psia) @17:46:28.88 Cartridge 42 filtering 155/1000ml at 0.63ml/s with 13.7psi vacuum 20.2m deep (44.6psia) @17:47:28.88 Cartridge 42 filtering 193/1000ml at 0.63ml/s with 13.1psi vacuum 19.4m deep (43.4psia) @17:48:28.88 Cartridge 42 filtering 230/1000ml at 0.63ml/s with 11.1psi vacuum 20.6m deep (45.1psia) @17:49:28.88 Cartridge 42 filtering 268/1000ml at 0.63ml/s with 13.7psi vacuum 20.1m deep (44.4psia) @17:50:28.88 Cartridge 42 filtering 306/1000ml at 0.63ml/s with 8.5psi vacuum 24.7m deep (51.2psia) @17:51:28.88 Cartridge 42 filtering 344/1000ml at 0.63ml/s with 12.1psi vacuum 19.3m deep (43.3psia) @17:52:29.38 Cartridge 42 filtering 382/1000ml at 0.63ml/s with 6.5psi vacuum 28.7m deep (57.1psia) @17:53:28.88 Cartridge 42 filtering 419/1000ml at 0.62ml/s with 12.6psi vacuum 24.2m deep (50.4psia) @17:54:28.88 Cartridge 42 filtering 457/1000ml at 0.63ml/s with 8.7psi vacuum 25.8m deep (52.8psia) @17:55:28.88 Cartridge 42 filtering 495/1000ml at 0.63ml/s with 12.8psi vacuum 21.6m deep (46.6psia) @17:56:28.88 Cartridge 42 filtering 532/1000ml at 0.63ml/s with 13.3psi vacuum 16.5m deep (39.1psia) @17:57:28.88 Cartridge 42 filtering 570/1000ml at 0.63ml/s with 14.2psi vacuum 19.6m deep (43.6psia) @17:58:28.88 Cartridge 42 filtering 607/1000ml at 0.62ml/s with 13.0psi vacuum 20.5m deep (45.0psia) @17:59:28.88 Cartridge 42 filtering 645/1000ml at 0.62ml/s with 8.0psi vacuum 26.5m deep (53.9psia) @18:00:28.88 Cartridge 42 filtering 682/1000ml at 0.62ml/s with 10.0psi vacuum 26.5m deep (53.8psia) @18:01:28.88 Cartridge 42 filtering 720/1000ml at 0.63ml/s with 9.1psi vacuum 21.2m deep (46.0psia) @18:02:28.87 Cartridge 42 filtering 757/1000ml at 0.63ml/s with 13.3psi vacuum 21.9m deep (47.1psia) @18:03:28.88 Cartridge 42 filtering 795/1000ml at 0.63ml/s with 6.5psi vacuum 22.2m deep (47.5psia) @18:04:28.88 Cartridge 42 filtering 833/1000ml at 0.63ml/s with 12.1psi vacuum 15.1m deep (37.0psia) @18:05:28.88 Cartridge 42 filtering 870/1000ml at 0.62ml/s with 6.6psi vacuum 22.1m deep (47.3psia) @18:06:28.88 Cartridge 42 filtering 908/1000ml at 0.63ml/s with 7.2psi vacuum 23.1m deep (48.8psia) @18:07:28.88 Cartridge 42 filtering 945/1000ml at 0.62ml/s with 7.3psi vacuum 23.4m deep (49.3psia) @18:08:28.88 Cartridge 42 filtering 982/1000ml at 0.62ml/s with 11.2psi vacuum 19.5m deep (43.5psia) @18:08:57.50 Abs.stop @18:08:57.57 Intake.close @18:08:57.63 Exhaust.close @18:08:57.82 Toroid.in :hold @18:08:57.88 Toroid.seek :bypass @18:09:01.47 Toroid.in :default @18:09:01.53 Toroid.seek :clear @18:09:05.12 Sampled 1000.0ml @18:09:05.53 Handoff.select :waste @18:09:05.66 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:09:12.26 Syringe tare is 51% of full scale at 22C (drifted -0%) @18:09:12.28 PinPort.in :locking @18:09:12.36 PinPort.seek :locked @18:09:13.76 Archive.in :fast @18:09:13.83 Lysis2.in :fast @18:09:13.88 Lysis1.seek 1250uL @18:09:13.96 Archive.seek :empty @18:09:14.06 Lysis2.seek :empty @18:09:22.52 Evacuating puck with filter bubble point of 20psi @18:09:22.55 PinPort.seek :locked @18:09:22.65 Handoff.select :waste @18:09:22.67 Plunge.seek :puckBottom @18:09:23.99 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:09:30.56 Air syringe pumping up to 1 strokes to reach 4psi @18:09:30.57 Air stroke #1 of 1 @18:09:30.67 Air.in :pressurize @18:09:30.73 Air.seek :pressurized @18:09:35.96 -0.0psi after pressurize @18:09:35.98 Air.in :compress @18:09:36.05 Air.seek :evac @18:09:42.65 1.1psi after compress @18:09:42.86 Reached 3.7 of 4.0psi after pumping 1775uL (1.00 strokes) @18:09:42.88 Air.in :default @18:09:42.95 Air.hold @18:09:43.08 Handoff.select :blocked @18:09:43.22 Plunge.seek :puckTop @18:09:44.09 Overpressure in PROCESSING -- Waste port clogged (2.4psi) @18:09:44.11 Waiting to Resume from Checkpoint "Air syringe pumping up to 1 strokes to reach 4psi" @18:27:36.43PDT20-Jul-26
Environmental Sample Processor built 17-Jul-26 (24b6d875) @18:27:39.79 >BREAK< ESP3 Gateway v1.44 for core bus @18:27:39.81 >BREAK< ESP3 Gateway v1.44 for cartridge bus @18:27:42.64 Configuration for honu by brent@mbari.org 7/17/26 /home/esp/esp2/type/auv/honu/configure.rb @18:27:43.19 Sea pressure (Abs) tare is 6% of full scale @18:27:47.76 Polling Can every 2:00 @18:27:47.80 Waiting for espclients... @18:27:49.59 Session Begun @18:29:10.40 -> ESP.ready! @18:29:10.45 Gate.power :main,:ON,limit to 5000mA @18:29:10.46 Gate.power :analytic1,:ON,limit to 4000mA @18:29:10.49 Gate.power :analytic2,:ON,limit to 4000mA @18:29:10.51 Gate.power :analytic3,:ON,limit to 4000mA @18:29:10.65 Powering core bus @18:29:12.98 UserAbort in nadia -- User Abort!! @18:29:16.05 -> ESP.configure @18:29:16.83 Gate.power :analytic1,:ON,limit to 4000mA @18:29:16.84 Gate.power :analytic2,:ON,limit to 4000mA @18:29:16.87 Gate.power :analytic3,:ON,limit to 4000mA @18:29:16.94 Posix::Error in Probe-AM1 -- serial port AM1 already locked by process 1288 !! @18:29:16.96 Gate.power :core,:OFF @18:29:17.03 Powering core bus @18:29:17.85 AnalyticModule::Error in Probe-AM1 -- No Response to BREAK @18:29:19.77 Plunge.configure :default @18:29:19.85 SamplePump.configure :default @18:29:19.93 Toroid.configure :default @18:29:20.00 Carousel.configure :default @18:29:20.08 Abs.configure :default @18:29:20.16 PhaseSeparator.configure :default @18:29:20.24 Archive.configure :default @18:29:20.32 Air.configure :default @18:29:20.37 Lysis1.configure :default @18:29:20.43 Lysis2.configure :default @18:29:20.51 PinPort.configure :default @18:29:20.57 All elf controllers running firmware version 4.94 @18:29:20.59 Gate.power :motor,:ON,limit to 5000mA @18:29:20.62 AnalyticModule::Unknown in nadia -- SPR @18:29:25.03 -> PV.locked @18:29:25.06 Plunge.home.seek :locked Plunge Valve (PV) locked @18:29:35.39 -> 5.times {PhytoSampler.filter 25; PhytoSamper.evacSWpumpTo; PV.locked} @18:29:35.54 Slide::Error in nadia -- Lysis Syringe #1 (LyS1) Not yet Homed! @18:30:25.51 -> Handoff.waste @18:30:25.53 Handoff.select :waste Handoff Valve::Manifold selects waste @18:30:37.41 -> Syringe.psi -0.0539214347730592 @18:32:33.33 -> ESP.ready1 @18:32:33.35 NoMethodError in nadia -- undefined method `ready1' for ESP:Module @18:32:35.60 -> ESP.ready! @18:32:36.14 Carousel.home.jog 0 @18:32:36.16 Toroid.home.jog 0 @18:32:36.37 PinPort.home.jog 0 @18:32:36.44 Lysis1.home.jog 0 @18:32:36.47 Air.home.jog 0 @18:32:36.49 Archive.home.jog 0 @18:32:36.54 Lysis2.home.jog 0 @18:32:36.90 Toroid.in :homing @18:32:37.11 PinPort.in :homing @18:32:37.18 Lysis1.in :homing @18:32:37.22 Toroid.jog 1366 @18:32:37.33 PinPort.jog 3150 @18:32:37.37 Lysis1.jog 4378 @18:32:37.57 Lysis2.in :homing @18:32:37.60 Air.in :homing @18:32:37.61 Archive.in :homing @18:32:37.73 Lysis2.jog 9650 @18:32:37.78 Air.jog 9600 @18:32:37.84 Archive.jog 9650 @18:32:40.00 Lysis1.setRawPosition! 50 @18:32:40.05 Lysis1.seek :home @18:32:40.33 Lysis1.in :default @18:32:41.78 PinPort.setRawPosition! 3150 @18:32:41.84 PinPort.seek :home @18:32:41.93 Toroid.setRawPosition! 0 @18:32:41.98 Toroid.seek :home @18:32:42.06 Toroid.in :default @18:32:42.84 Lysis2.setRawPosition! 50 @18:32:42.91 Lysis2.seek :home @18:32:43.00 Archive.setRawPosition! 50 @18:32:43.02 Air.setRawPosition! 0 @18:32:43.11 Archive.seek :home @18:32:43.14 Air.seek :home @18:32:43.30 Air.in :default @18:32:43.32 Lysis2.in :default @18:32:43.44 Archive.in :default @18:32:46.21 PinPort.in :default @18:32:46.54 Toroid.seek :clear @18:32:46.67 PinPort.in :unlocking @18:32:46.75 PinPort.seek :clear @18:32:50.19 Air.seek :clear @18:32:50.28 Lysis1.seek :clear @18:32:51.01 Lysis2.seek :clear @18:32:51.80 Archive.seek :clear @18:32:52.59 Carousel.in :default @18:32:52.70 Carousel.home.seek 42 Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @27psia PhaseSeparator = Intake Head Pressure @3.86psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:33:05.21 -> Cartridge.lock @18:33:05.31 Carousel.reconfigure :align @18:33:05.42 Lysis1.in :fast @18:33:05.48 Lysis1.seek :locked @18:33:08.16 Air.in :fast @18:33:08.21 Air.seek :locked @18:33:09.28 Carousel.reconfigure :locked @18:33:09.42 Powering cartridge bus @18:33:12.05 Gate.setIR 1750,5000,0,6 @18:33:12.17 Gate.setIR 0,5000,0,6 @18:33:12.24 PuckHeater.configure :default @18:33:12.30 Gate.power :heat,:ON,limit to 5000mA @18:33:12.49 Mounted lysis Cartridge board#J60008 @18:33:12.53 Using default cartridge heat loss curve for HeatingFunctionInCarousel :lyseHold_phyto @18:33:19.80 -> 5.times {PhytoSampler.filter 25; PhytoSamper.evacSWpumpTo; PV.locked} @18:33:20.00 Air.in :default @18:33:20.13 Priming sample loop w/200ml, bypass w/10ml @18:33:20.14 Air.seek :sealed @18:33:20.18 SamplePump.setPosition! 0ml @18:33:20.29 Bypass.open @18:33:22.53 Intake.open @18:33:24.24 Sea 21.9m deep (47.0psia) @18:33:24.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:33:27.37 Vacuum tare is 63% of full scale at 21C (drifted 12%) @18:33:27.51 Intake.close @18:33:27.57 Bypass.close @18:33:27.63 Handoff.select :close @18:33:29.27 Verifying toroid holds at least 0.6..0.85 of 46.9psia @18:33:29.29 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @18:33:33.88 Toroid pressure rose 1% to 47.3psia @18:33:33.89 Bypass.open @18:33:35.39 Intake.open @18:33:37.79 Abs.in :default @18:33:37.85 Abs.hold @18:33:38.12 SamplePump.in :start @18:33:38.19 SamplePump.seek 10ml,1:10 @18:33:38.81 SamplePump.configure :prime @18:33:51.71 Abs.stop @18:33:51.77 SamplePump.setPosition! 0ml @18:33:51.83 Exhaust.open @18:33:54.03 Bypass.close @18:33:56.36 Abs.in :default @18:33:56.41 Abs.hold @18:33:56.56 Sea 22.0m deep (47.2psia) @18:33:56.72 SamplePump.in :start @18:33:56.79 SamplePump.seek 200ml,13:50 @18:33:57.42 SamplePump.configure :prime @18:38:19.54 Abs.stop @18:38:19.67 Bypass.open @18:38:21.19 Intake.close @18:38:22.93 Toroid.in :hold @18:38:22.98 Toroid.seek :crack @18:38:25.46 Toroid.seek :cartridge @18:38:27.57 Toroid.in :default @18:38:27.64 Toroid.seek :clear @18:38:31.56 Exhaust.close @18:38:31.61 Bypass.close @18:38:33.15 Verifying cartridge holds at least 0.65..0.85 of 47.4psia @18:38:33.17 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @18:38:37.81 Sampling 25.0ml @18:38:37.82 SamplePump.setPosition! 0ml @18:38:37.89 Exhaust.open @18:38:39.32 Intake.open @18:38:41.51 Abs.in :default @18:38:41.64 Abs.hold @18:38:41.78 Sea 22.1m deep (47.3psia) @18:38:41.96 SamplePump.in :start @18:38:42.03 SamplePump.seek 25ml,1:54 @18:38:42.65 SamplePump.configure :sample @18:38:45.04 Cartridge 42 filtering 1/25ml at 0.61ml/s with 7.8psi vacuum 30.8m deep (60.2psia) @18:39:23.00 Abs.stop @18:39:23.08 Intake.close @18:39:23.14 Exhaust.close @18:39:23.32 Toroid.in :hold @18:39:23.39 Toroid.seek :bypass @18:39:26.98 Toroid.in :default @18:39:27.05 Toroid.seek :clear @18:39:30.63 Sampled 25.0ml @18:39:30.80 NameError in nadia -- uninitialized constant PhytoSamper @18:39:59.99 -> 5.times {PhytoSampler.filter 25; PhytoSampler.evacSWpumpTo; PV.locked} @18:40:00.18 Air.seek :sealed @18:40:00.34 Toroid.in :hold @18:40:00.41 Toroid.seek :cartridge @18:40:04.18 Toroid.in :default @18:40:04.24 Toroid.seek :clear @18:40:08.00 Sampling 25.0ml @18:40:08.01 SamplePump.setPosition! 0ml @18:40:08.09 Exhaust.open @18:40:09.58 Intake.open @18:40:11.44 Abs.in :default @18:40:11.49 Abs.hold @18:40:11.62 Sea 22.0m deep (47.2psia) @18:40:11.80 SamplePump.in :start @18:40:11.86 SamplePump.seek 25ml,1:54 @18:40:12.49 SamplePump.configure :sample @18:40:14.88 Cartridge 42 filtering 1/25ml at 0.69ml/s with 12.6psi vacuum 18.6m deep (42.2psia) @18:40:52.44 Abs.stop @18:40:52.52 Intake.close @18:40:52.56 Exhaust.close @18:40:52.77 Toroid.in :hold @18:40:52.83 Toroid.seek :bypass @18:40:56.43 Toroid.in :default @18:40:56.50 Toroid.seek :clear @18:41:00.07 Sampled 25.0ml @18:41:00.67 Evacuating puck with filter bubble point of 20psi @18:41:00.69 PinPort.in :locking @18:41:00.76 PinPort.seek :locked @18:41:01.59 Handoff.select :waste @18:41:01.73 Plunge.seek :puckBottom @18:41:03.03 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:41:14.10 Air syringe pumping up to 1 strokes to reach 4psi @18:41:14.11 Air stroke #1 of 1 @18:41:14.21 Air.in :pressurize @18:41:14.27 Air.seek :pressurized @18:41:19.51 -0.5psi after pressurize @18:41:19.53 Air.in :compress @18:41:19.60 Air.seek :evac @18:41:26.21 1.1psi after compress @18:41:36.78 -0.6psi after compress (stable) @18:41:36.79 Air.in :default @18:41:36.86 Air.hold @18:41:37.04 at -0.4psi Opened to waste @18:41:37.06 Handoff.select :blocked @18:41:37.19 Plunge.seek :puckTop @18:41:38.07 Air syringe pumping up to 8 strokes to reach 20psi @18:41:38.08 Air stroke #1 of 8 @18:41:38.18 Air.in :compress @18:41:38.24 Air.seek :evac @18:41:38.37 0.6psi after compress @18:41:48.94 0.8psi after compress (stable) @18:41:48.97 Air.in :gulp @18:41:49.03 Air.seek :topVent @18:41:55.47 0.8psi after gulp @18:42:01.05 1.1psi after gulp (stable) @18:42:01.06 Air stroke #2 of 8 @18:42:01.16 Air.in :pressurize @18:42:01.22 Air.seek :pressurized @18:42:07.59 0.9psi after pressurize @18:42:07.62 Air.in :compress @18:42:07.69 Air.seek :evac @18:42:14.29 11.7psi after compress @18:42:25.87 3.6psi after compress (stable) @18:42:25.89 Air.in :gulp @18:42:25.97 Air.seek :topVent @18:42:32.80 3.8psi after gulp @18:42:38.95 3.7psi after gulp (stable) @18:42:38.96 Air stroke #3 of 8 @18:42:39.07 Air.in :pressurize @18:42:39.13 Air.seek :pressurized @18:42:45.50 3.5psi after pressurize @18:42:45.52 Air.in :compress @18:42:45.59 Air.seek :evac @18:42:52.19 3.3psi after compress @18:43:02.77 4.4psi after compress (stable) @18:43:02.79 Air.in :gulp @18:43:02.85 Air.seek :topVent @18:43:09.29 4.6psi after gulp @18:43:14.87 4.6psi after gulp (stable) @18:43:14.88 Air stroke #4 of 8 @18:43:14.97 Air.in :pressurize @18:43:15.03 Air.seek :pressurized @18:43:21.40 4.5psi after pressurize @18:43:21.42 Air.in :compress @18:43:21.50 Air.seek :evac @18:43:28.11 9.4psi after compress @18:43:41.68 1.7psi after compress (stable) @18:43:41.71 Air.in :gulp @18:43:41.78 Air.seek :topVent @18:43:48.23 1.9psi after gulp @18:43:53.80 1.7psi after gulp (stable) @18:43:53.81 Air stroke #5 of 8 @18:43:53.92 Air.in :pressurize @18:43:53.97 Air.seek :pressurized @18:44:00.34 1.6psi after pressurize @18:44:00.36 Air.in :compress @18:44:00.43 Air.seek :evac @18:44:07.03 4.8psi after compress @18:44:17.60 5.6psi after compress (stable) @18:44:17.62 Air.in :gulp @18:44:17.70 Air.seek :topVent @18:44:24.15 5.8psi after gulp @18:44:29.72 5.6psi after gulp (stable) @18:44:29.73 Air stroke #6 of 8 @18:44:29.83 Air.in :pressurize @18:44:29.89 Air.seek :pressurized @18:44:36.26 5.4psi after pressurize @18:44:36.28 Air.in :compress @18:44:36.35 Air.seek :evac @18:44:42.96 8.6psi after compress @18:44:53.53 9.6psi after compress (stable) @18:44:53.55 Air.in :gulp @18:44:53.63 Air.seek :topVent @18:45:00.08 9.7psi after gulp @18:45:05.65 9.6psi after gulp (stable) @18:45:05.66 Air stroke #7 of 8 @18:45:05.76 Air.in :pressurize @18:45:05.82 Air.seek :pressurized @18:45:12.60 9.6psi after pressurize @18:45:12.62 Air.in :compress @18:45:12.69 Air.seek :evac @18:45:19.30 12.6psi after compress @18:45:29.87 13.3psi after compress (stable) @18:45:29.89 Air.in :gulp @18:45:29.96 Air.seek :topVent @18:45:36.41 13.4psi after gulp @18:45:41.98 13.5psi after gulp (stable) @18:45:41.99 Air stroke #8 of 8 @18:45:42.09 Air.in :pressurize @18:45:42.15 Air.seek :pressurized @18:45:48.52 13.3psi after pressurize @18:45:48.54 Air.in :compress @18:45:48.62 Air.seek :evac @18:45:55.22 16.3psi after compress @18:46:05.79 17.1psi after compress (stable) @18:46:05.81 Air.in :default @18:46:05.87 Air.hold @18:46:06.00 Handoff.select :blocked @18:46:06.11 Underpressure in nadia -- Reached only 16.9 of 20.0psi after pumping 14203uL @18:47:08.40 -> Syringe.psi 17.3899078348123 @18:47:42.22 -> AirS.clear @18:47:42.25 Air.seek :clear Air Syringe (AirS) clear @18:48:04.14 -> Syringe.psi 17.1187040215414 @18:48:13.02 -> AirS.pressurized @18:48:13.05 Air.seek :pressurized Air Syringe (AirS) pressurized @18:48:30.30 -> Syringe.psi 17.2054892417881 @18:48:50.81 -> AirS.evac @18:48:50.85 Air.seek :evac Air Syringe (AirS) at evac @18:49:00.37 -> Syringe.psi 20.8070758820259 @18:49:11.07 -> Handoff.waste @18:49:11.09 Handoff.select :waste Handoff Valve::Manifold selects waste @18:49:21.95 -> Cartridge.lock @18:49:21.99 Gate.power :heat,:OFF @18:49:22.00 Gate.power :cartridge,:OFF @18:49:22.08 Carousel.reconfigure :align @18:49:22.35 Carousel.reconfigure :locked @18:49:23.55 Powering cartridge bus @18:49:26.18 Gate.setIR 1750,5000,0,6 @18:49:26.30 Gate.setIR 0,5000,0,6 @18:49:26.37 PuckHeater.configure :default @18:49:26.43 Gate.power :heat,:ON,limit to 5000mA @18:49:26.58 Mounted lysis Cartridge board#J60008 @18:49:26.62 Using default cartridge heat loss curve for HeatingFunctionInCarousel :lyseHold_phyto @18:49:30.01 -> ESP.reready! @18:49:30.04 Plunge.in :default @18:49:30.09 Toroid.in :default @18:49:30.18 Carousel.in :default @18:49:30.27 Archive.in :default @18:49:30.37 Air.in :default @18:49:30.42 Lysis1.in :default @18:49:30.45 Plunge.home.jog 0 @18:49:30.51 Lysis2.in :default @18:49:30.55 Toroid.home.jog 0 @18:49:30.59 PinPort.in :default @18:49:30.65 Carousel.home.jog 0 @18:49:30.75 Archive.home.jog 0 @18:49:30.84 Air.home.jog 0 @18:49:30.91 Lysis1.home.jog 0 @18:49:31.21 PinPort.home.jog 0 @18:49:31.28 Lysis2.home.jog 0 @18:49:31.86 Plunge.in :homing @18:49:32.05 Plunge.jog 2390 @18:49:32.10 PinPort.in :homing @18:49:32.13 Lysis2.in :homing @18:49:32.15 Lysis1.in :homing @18:49:32.18 Air.in :homing @18:49:32.21 Toroid.in :homing @18:49:32.25 Archive.in :homing @18:49:32.50 PinPort.jog 3150 @18:49:32.55 Lysis2.jog 1622 @18:49:32.59 Air.jog 9600 @18:49:32.62 Lysis1.jog 2268 @18:49:32.65 Toroid.jog 1363 @18:49:32.72 Archive.jog 302 @18:49:33.47 Archive.setRawPosition! 50 @18:49:33.53 Archive.seek :home @18:49:33.81 Archive.in :default @18:49:34.05 Plunge.setRawPosition! 50 @18:49:34.11 Plunge.seek :home @18:49:34.69 Lysis2.setRawPosition! 50 @18:49:34.71 Lysis1.setRawPosition! 50 @18:49:34.80 Lysis2.seek :home @18:49:34.84 Lysis1.seek :home @18:49:35.00 Plunge.in :default @18:49:35.14 Lysis2.in :default @18:49:35.23 Lysis1.in :default @18:49:37.05 PinPort.setRawPosition! 3150 @18:49:37.10 PinPort.seek :home @18:49:37.37 Toroid.setRawPosition! 0 @18:49:37.43 Toroid.seek :home @18:49:37.51 Toroid.in :default @18:49:37.79 Air.setRawPosition! 0 @18:49:37.84 Air.seek :home @18:49:37.93 Air.in :default @18:49:41.47 PinPort.in :default @18:49:41.80 Toroid.seek :clear @18:49:41.93 PinPort.in :unlocking @18:49:41.99 PinPort.seek :clear @18:49:45.40 Air.seek :clear @18:49:45.49 Lysis1.seek :clear @18:49:46.21 Lysis2.seek :clear @18:49:46.99 Archive.seek :clear @18:49:47.79 Carousel.in :default @18:49:47.90 Carousel.home.seek 42 Plunge = Plunge Valve (PV) at home Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) at 25ml Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 42 Abs = Absolute Pressure (Leak) Sensor @42psia PhaseSeparator = Intake Head Pressure @3.86psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear PuckHeater = Puck Heater at 20.7C @18:50:14.29 -> Cart.lock @18:50:14.33 Gate.power :heat,:OFF @18:50:14.35 Gate.power :cartridge,:OFF @18:50:14.44 Carousel.reconfigure :align @18:50:14.55 Lysis1.in :fast @18:50:14.61 Lysis1.seek :locked @18:50:17.22 Plunge.seek :locked @18:50:17.35 Air.in :fast @18:50:17.42 Air.seek :locked @18:50:18.49 Carousel.reconfigure :locked @18:50:18.64 Powering cartridge bus @18:50:21.26 Gate.setIR 1750,5000,0,6 @18:50:21.38 Gate.setIR 0,5000,0,6 @18:50:21.45 PuckHeater.configure :default @18:50:21.52 Gate.power :heat,:ON,limit to 5000mA @18:50:21.67 Mounted lysis Cartridge board#J60008 @18:50:21.71 Using default cartridge heat loss curve for HeatingFunctionInCarousel :lyseHold_phyto @18:50:22.84 -> Intake.open @18:50:22.86 Intake.open Intake is opened @18:53:16.18 -> Intake.close @18:53:16.20 Intake.close Intake is closed @18:53:22.22 -> Intake.open @18:53:22.24 Intake.open Intake is opened @18:53:29.61 -> Intake.close @18:53:29.63 Intake.close Intake is closed @18:53:39.79 -> Intake.open @18:53:39.81 Intake.open Intake is opened @18:53:44.09 -> Intake.close @18:53:44.11 Intake.close Intake is closed @18:54:01.31 -> 5.times {PhytoSampler.filter 25; PhytoSampler.evacSWpumpTo; PV.locked} @18:54:01.97 Air.in :default @18:54:02.03 Air.seek :sealed @18:54:04.85 Toroid.in :hold @18:54:04.91 Toroid.seek :cartridge @18:54:08.68 Toroid.in :default @18:54:08.75 Toroid.seek :clear @18:54:12.50 Sampling 25.0ml @18:54:12.52 SamplePump.setPosition! 0ml @18:54:12.58 Exhaust.open @18:54:14.10 Intake.open @18:54:15.80 Abs.in :default @18:54:15.85 Abs.hold @18:54:15.98 Sea 21.9m deep (47.0psia) @18:54:16.16 SamplePump.in :start @18:54:16.23 SamplePump.seek 25ml,1:54 @18:54:16.85 SamplePump.configure :sample @18:54:19.24 Cartridge 42 filtering 2/25ml at 0.63ml/s with 13.8psi vacuum 20.3m deep (44.7psia) @18:54:56.22 Abs.stop @18:54:56.30 Intake.close @18:54:56.36 Exhaust.close @18:54:56.41 Handoff.select :close @18:54:56.65 Toroid.in :hold @18:54:56.72 Toroid.seek :bypass @18:55:00.32 Toroid.in :default @18:55:00.38 Toroid.seek :clear @18:55:03.96 Sampled 25.0ml @18:55:04.13 Evacuating puck with filter bubble point of 20psi @18:55:04.15 PinPort.in :locking @18:55:04.21 PinPort.seek :locked @18:55:05.03 Handoff.select :waste @18:55:05.17 Plunge.seek :puckBottom @18:55:06.47 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:55:13.04 Air syringe pumping up to 1 strokes to reach 4psi @18:55:13.05 Air stroke #1 of 1 @18:55:13.15 Air.in :pressurize @18:55:13.21 Air.seek :pressurized @18:55:18.44 -0.7psi after pressurize @18:55:18.47 Air.in :compress @18:55:18.54 Air.seek :evac @18:55:25.14 0.9psi after compress @18:55:35.71 -0.2psi after compress (stable) @18:55:35.73 Air.in :default @18:55:35.80 Air.hold @18:55:36.04 at -0.1psi Opened to waste @18:55:36.05 Handoff.select :blocked @18:55:36.19 Plunge.seek :puckTop @18:55:37.07 Air syringe pumping up to 8 strokes to reach 20psi @18:55:37.08 Air stroke #1 of 8 @18:55:37.18 Air.in :compress @18:55:37.23 Air.seek :evac @18:55:37.37 0.1psi after compress @18:55:47.95 0.2psi after compress (stable) @18:55:47.97 Air.in :gulp @18:55:48.05 Air.seek :topVent @18:55:54.49 0.2psi after gulp @18:56:00.07 0.2psi after gulp (stable) @18:56:00.08 Air stroke #2 of 8 @18:56:00.59 Air.in :pressurize @18:56:00.73 Air.seek :pressurized @18:56:07.10 0.0psi after pressurize @18:56:07.13 Air.in :compress @18:56:07.20 Air.seek :evac @18:56:13.81 3.5psi after compress @18:56:24.39 4.5psi after compress (stable) @18:56:24.41 Air.in :gulp @18:56:24.47 Air.seek :topVent @18:56:30.92 4.6psi after gulp @18:56:36.50 4.3psi after gulp (stable) @18:56:36.51 Air stroke #3 of 8 @18:56:36.61 Air.in :pressurize @18:56:36.67 Air.seek :pressurized @18:56:43.05 4.2psi after pressurize @18:56:43.07 Air.in :compress @18:56:43.15 Air.seek :evac @18:56:49.75 7.6psi after compress @18:57:00.32 8.6psi after compress (stable) @18:57:00.41 Air.in :gulp @18:57:00.48 Air.seek :topVent @18:57:06.94 8.7psi after gulp @18:57:12.53 8.6psi after gulp (stable) @18:57:12.54 Air stroke #4 of 8 @18:57:12.64 Air.in :pressurize @18:57:12.70 Air.seek :pressurized @18:57:19.07 8.5psi after pressurize @18:57:19.09 Air.in :compress @18:57:19.16 Air.seek :evac @18:57:25.76 11.9psi after compress @18:57:36.34 12.6psi after compress (stable) @18:57:36.36 Air.in :gulp @18:57:36.43 Air.seek :topVent @18:57:42.89 12.6psi after gulp @18:57:48.46 12.8psi after gulp (stable) @18:57:48.54 Air stroke #5 of 8 @18:57:48.64 Air.in :pressurize @18:57:48.71 Air.seek :pressurized @18:57:55.07 12.7psi after pressurize @18:57:55.09 Air.in :compress @18:57:55.16 Air.seek :evac @18:58:01.77 15.6psi after compress @18:58:12.35 16.6psi after compress (stable) @18:58:12.37 Air.in :gulp @18:58:12.44 Air.seek :topVent @18:58:18.90 16.6psi after gulp @18:58:24.47 16.6psi after gulp (stable) @18:58:24.48 Air stroke #6 of 8 @18:58:24.58 Air.in :pressurize @18:58:24.64 Air.seek :pressurized @18:58:31.01 16.5psi after pressurize @18:58:31.03 Air.in :compress @18:58:31.09 Air.seek :evac @18:58:37.70 19.7psi after compress @18:58:37.91 Reached 20.2 of 20.0psi after pumping 10652uL (6.00 strokes) @18:58:37.92 Air.in :default @18:58:37.99 Air.hold @18:58:38.08 Plunge.seek :puckBottom @18:58:38.94 delay 3 seconds @18:58:41.96 Handoff.select :waste @18:58:42.09 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:58:54.16 delay 1:00 @18:59:54.24 Handoff.select :blocked @18:59:54.37 Waiting up to 3:00 for Syringe to stabilize +/-1.50psi @19:00:16.95 Handoff.select :waste @19:00:17.16 -0.5psi at evacEnd @19:00:17.18 Plunge.seek :pTop @19:00:18.05 delay 5 seconds @19:00:23.08 Air.in :glide @19:00:23.13 Air.seek :fullVent @19:00:24.77 delay 2 seconds @19:00:26.80 Plunge.seek :pBottom @19:00:27.66 Seawater evacuated, Pressure above/below filter released. @19:00:27.68 Plunge.seek :locked @19:00:29.17 Air.in :default @19:00:29.23 Air.seek :sealed @19:00:43.57 Toroid.in :hold @19:00:43.63 Toroid.seek :cartridge @19:00:47.40 Toroid.in :default @19:00:47.46 Toroid.seek :clear @19:00:51.21 Sampling 25.0ml @19:00:51.23 SamplePump.setPosition! 0ml @19:00:51.30 Exhaust.open @19:00:52.75 Intake.open @19:00:54.48 Abs.in :default @19:00:54.53 Abs.hold @19:00:54.67 Sea 21.7m deep (46.8psia) @19:00:54.84 SamplePump.in :start @19:00:54.92 SamplePump.seek 25ml,1:54 @19:00:55.54 SamplePump.configure :sample @19:00:57.92 Cartridge 42 filtering 1/25ml at 0.55ml/s with 12.4psi vacuum 21.4m deep (46.2psia) @19:01:34.66 Abs.stop @19:01:34.73 Intake.close @19:01:34.79 Exhaust.close @19:01:34.96 Handoff.select :close @19:01:35.16 Toroid.in :hold @19:01:35.23 Toroid.seek :bypass @19:01:38.82 Toroid.in :default @19:01:38.89 Toroid.seek :clear @19:01:42.46 Sampled 25.0ml @19:01:42.63 Evacuating puck with filter bubble point of 20psi @19:01:42.66 PinPort.seek :locked @19:01:42.75 Handoff.select :waste @19:01:42.88 Plunge.seek :puckBottom @19:01:44.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:01:53.76 Air syringe pumping up to 1 strokes to reach 4psi @19:01:53.77 Air stroke #1 of 1 @19:01:53.87 Air.in :pressurize @19:01:53.94 Air.seek :pressurized @19:01:59.19 -0.5psi after pressurize @19:01:59.21 Air.in :compress @19:01:59.28 Air.seek :evac @19:02:05.88 -0.7psi after compress @19:02:16.45 -0.5psi after compress (stable) @19:02:16.47 Air.in :default @19:02:16.52 Air.hold @19:02:16.71 at -0.6psi Opened to waste @19:02:16.73 Handoff.select :blocked @19:02:16.87 Plunge.seek :puckTop @19:02:17.73 Air syringe pumping up to 8 strokes to reach 20psi @19:02:17.75 Air stroke #1 of 8 @19:02:17.85 Air.in :compress @19:02:17.91 Air.seek :evac @19:02:18.04 -0.6psi after compress @19:02:28.62 -0.4psi after compress (stable) @19:02:28.64 Air.in :gulp @19:02:28.71 Air.seek :topVent @19:02:35.17 -0.3psi after gulp @19:02:40.74 -0.3psi after gulp (stable) @19:02:40.75 Air stroke #2 of 8 @19:02:40.85 Air.in :pressurize @19:02:40.90 Air.seek :pressurized @19:02:47.27 -0.5psi after pressurize @19:02:47.30 Air.in :compress @19:02:47.37 Air.seek :evac @19:02:53.97 3.3psi after compress @19:03:04.55 4.3psi after compress (stable) @19:03:04.57 Air.in :gulp @19:03:04.65 Air.seek :topVent @19:03:11.11 4.4psi after gulp @19:03:16.68 4.4psi after gulp (stable) @19:03:16.75 Air stroke #3 of 8 @19:03:16.86 Air.in :pressurize @19:03:16.92 Air.seek :pressurized @19:03:23.29 4.2psi after pressurize @19:03:23.32 Air.in :compress @19:03:23.38 Air.seek :evac @19:03:29.98 7.8psi after compress @19:03:40.55 9.2psi after compress (stable) @19:03:40.58 Air.in :gulp @19:03:40.64 Air.seek :topVent @19:03:47.09 9.0psi after gulp @19:03:52.66 9.1psi after gulp (stable) @19:03:52.67 Air stroke #4 of 8 @19:03:52.77 Air.in :pressurize @19:03:52.84 Air.seek :pressurized @19:03:59.22 9.0psi after pressurize @19:03:59.24 Air.in :compress @19:03:59.30 Air.seek :evac @19:04:05.91 12.4psi after compress @19:04:16.49 13.5psi after compress (stable) @19:04:16.57 Air.in :gulp @19:04:16.64 Air.seek :topVent @19:04:23.10 13.6psi after gulp @19:04:31.17 13.5psi after gulp (stable) @19:04:31.18 Air stroke #5 of 8 @19:04:31.28 Air.in :pressurize @19:04:31.34 Air.seek :pressurized @19:04:37.70 13.4psi after pressurize @19:04:37.72 Air.in :compress @19:04:37.79 Air.seek :evac @19:04:44.39 17.0psi after compress @19:04:55.73 Session Begun @19:04:55.83 18.0psi after compress (stable) @19:04:55.86 Air.in :gulp @19:04:55.95 Air.seek :topVent @19:05:02.41 18.1psi after gulp @19:05:07.99 18.0psi after gulp (stable) @19:05:08.00 Air stroke #6 of 8 @19:05:08.12 Air.in :pressurize @19:05:08.19 Air.seek :pressurized @19:05:09.52 -> exit @19:05:09.54 Session Ended @19:05:14.58 17.9psi after pressurize @19:05:14.60 Air.in :compress @19:05:14.67 Air.seek :evac @19:05:20.50 Reached 20.3 of 20.0psi after pumping 10601uL (5.97 strokes) @19:05:20.54 Air.in :default @19:05:20.65 Air.hold @19:05:20.82 Plunge.seek :puckBottom @19:05:21.75 delay 3 seconds