@16:50:23.58PDT31-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @16:50:23.61 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:50:26.85 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @16:50:27.47 Sea pressure (Abs) tare is 6% of full scale @16:50:30.63 Polling Can every 2:00 @16:50:30.67 Loading /home/esp/esp2/bin/processAll mission @16:50:30.87 Sea pressure (Abs) tare is 6% of full scale @16:50:30.88 Sampling default goal volumes @16:50:30.90 Cartridge group: dry @16:50:31.06 Gate.power :analytic1,:ON,limit to 5000mA @16:50:31.07 Gate.power :analytic2,:ON,limit to 5000mA @16:50:31.08 Gate.power :analytic3,:ON,limit to 5000mA @16:50:31.76 Archive.configure :default @16:50:31.92 Air.configure :default @16:50:32.04 Lysis1.configure :default @16:50:32.17 Lysis2.configure :default @16:50:32.34 Plunge.configure :default @16:50:32.41 SPR Module 1, FW Rev. B, FPGA Rev. C @16:50:32.55
SamplePump.configure :default @16:50:32.73 Toroid.configure :default @16:50:32.79 Toroid.hold @16:50:32.85 Toroid.configure :default @16:50:33.01 Carousel.configure :default @16:50:33.07 Carousel.hold @16:50:33.14 Carousel.configure :default @16:50:33.29 Pressure.configure :default @16:50:33.44 ExtraPump.configure :default @16:50:33.56 PinPort.configure :default @16:50:33.62 All elf controllers running firmware version 4.83 @16:50:34.42 Toroid.seek :clear @16:50:34.50 Carousel.in :default @16:50:34.66 Carousel.home.seek 56 @16:50:34.94 Selecting Cartridge 56 @16:50:35.25 Carousel.seek 56 @16:50:35.47 Lysis1.in :fast @16:50:35.53 Lysis1.seek :locked @16:50:38.15 Plunge.seek :locked @16:50:38.31 Air.in :fast @16:50:38.37 Air.seek :locked @16:50:39.94
Carousel.reconfigure :locked @16:50:40.09 Powering cartridge bus @16:50:42.90 Gate.setIR 1750,5000,0,6 @16:50:43.02 Gate.setIR 0,5000,0,6 @16:50:43.09 PuckHeater.configure :default @16:50:43.15 Gate.power :heat,:ON,limit to 5000mA @16:50:43.91 Mounted lysis Cartridge board#Q05063 @16:50:43.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:50:44.27 Preparing to filter cartridge 56 @16:50:44.57 Priming sample loop w/100ml, bypass w/10ml @16:50:44.60 SamplePump.setPosition! 0ml @16:50:44.67 Exhaust.close @16:50:44.74 Bypass.open @16:50:46.24 Intake.open @16:50:46.99 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:50:49.58 Vacuum tare is 60% of full scale @16:50:49.73 Pressure.in :default @16:50:49.79 Pressure.hold @16:50:50.01 SamplePump.in :prime @16:50:50.08 SamplePump.seek 10ml,59 seconds @16:50:58.80 Pressure.stop @16:50:58.85 SamplePump.setPosition! 0ml @16:50:58.90 Exhaust.open @16:51:00.33 Bypass.close @16:51:01.87 Pressure.in :default @16:51:01.93 Pressure.hold @16:51:02.22 SamplePump.in :prime @16:51:02.29 SamplePump.seek 100ml,5:16 @16:51:43.54 SamplePump.stop @16:51:43.60 Intake.close @16:51:43.68 Exhaust.close @16:51:44.49 Pressure.stop @16:51:44.77 Cartridge::Sampler::Leaky in FILTERING -- Serious Leak or Stall -- 1.20ml/s made only 17.3psi vacuum after 49.0ml @16:51:44.79 Waiting to Resume from Checkpoint "Sample Pump (SP) pressureOutOfBounds at 48.98ml" @16:53:41.14 Session Begun @16:53:46.55 -> trouble >>> Thread[:FILTERING] is suspended <<< @16:50:44 Preparing to filter cartridge 56 @16:50:44 Priming sample loop w/100ml, bypass w/10ml @16:50:46 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:51:35 Sample Pump (SP) pressureOutOfBounds at 48.98ml @16:54:01.03 -> slot {[55,54] => { Type: :daSPR_phyto, State: :dry}, 56 => { Type: :daSPR_phyto, State: :leakyToroid}} @16:54:02.45 -> slot {[55,54] => { Type: :daSPR_phyto, State: :dry}, 56 => { Type: :daSPR_phyto, State: :leakyToroid}} @16:54:20.07 -> slot 56 =>[:dry, daSPR_phyto] {56 => { Type: :daSPR_phyto, State: :dry}} @16:54:22.78 -> slot {56..54 => { Type: :daSPR_phyto, State: :dry}} @16:54:47.22 -> Cartridge.unlock @16:54:47.23 Gate.power :heat,:OFF @16:54:47.28 Gate.power :cartridge,:OFF @16:54:47.40 Carousel.in :default @16:54:47.55 Carousel.home.seek 56 @16:54:47.99 Air.in :unlocking @16:54:48.67 Air.seek :clear @16:54:50.05 Lysis1.in :unlocking @16:54:50.27 Lysis1.seek :clear @16:54:50.31 Plunge.in :unlocking @16:54:50.47 Plunge.seek :clear @16:55:26.16 -> exit @16:55:26.18 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @16:56:14.97 Session Begun @16:56:20.39 -> MainThread.exit # @16:56:20.61 ThreadError in bill -- killed thread @16:56:20.72 Session Ended @16:56:43.69PDT31-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @16:56:43.72 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:56:46.93 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @16:56:47.51 Sea pressure (Abs) tare is 6% of full scale @16:56:50.65 Polling Can every 2:00 @16:56:50.69 Loading /home/esp/esp2/bin/processAll mission @16:56:50.90 Sea pressure (Abs) tare is 6% of full scale @16:56:50.91 Sampling default goal volumes @16:56:50.94 Cartridge group: dry @16:56:51.07 Gate.power :analytic1,:ON,limit to 5000mA @16:56:51.09 Gate.power :analytic2,:ON,limit to 5000mA @16:56:51.10 Gate.power :analytic3,:ON,limit to 5000mA @16:56:51.67 SPR Module 1, FW Rev. B, FPGA Rev. C @16:56:51.86
Archive.configure :default @16:56:51.92 Archive.hold @16:56:51.99 Archive.configure :default @16:56:52.14 Air.configure :default @16:56:52.20 Air.hold @16:56:52.27 Air.configure :default @16:56:52.40 Lysis1.configure :default @16:56:52.45 Lysis1.hold @16:56:52.52 Lysis1.configure :default @16:56:52.69 Lysis2.configure :default @16:56:52.75 Lysis2.hold @16:56:52.81 Lysis2.configure :default @16:56:52.98 Plunge.configure :default @16:56:53.04 Plunge.hold @16:56:53.11 Plunge.configure :default @16:56:53.27 SamplePump.configure :default @16:56:53.42 Toroid.configure :default @16:56:53.48 Toroid.hold @16:56:53.55 Toroid.configure :default @16:56:53.70 Carousel.configure :default @16:56:53.76 Carousel.hold @16:56:53.83 Carousel.configure :default @16:56:53.99 Pressure.configure :default @16:56:54.15 ExtraPump.configure :default @16:56:54.28 PinPort.configure :default @16:56:54.34 PinPort.hold @16:56:54.41 PinPort.configure :default @16:56:54.47 All elf controllers running firmware version 4.83 @16:56:55.30 Toroid.seek :clear @16:56:55.37 Carousel.in :default @16:56:55.49 Carousel.home.seek 56 @16:56:55.75 Selecting Cartridge 56 @16:56:56.04 Carousel.seek 56 @16:56:56.71 Lysis1.in :fast @16:56:56.81 Lysis1.seek :locked @16:56:59.45 Plunge.seek :locked @16:56:59.61 Air.in :fast @16:56:59.67 Air.seek :locked @16:57:01.25
Carousel.reconfigure :locked @16:57:01.40 Powering cartridge bus @16:57:04.05 Gate.setIR 1750,5000,0,6 @16:57:04.17 Gate.setIR 0,5000,0,6 @16:57:04.24 PuckHeater.configure :default @16:57:04.30 Gate.power :heat,:ON,limit to 5000mA @16:57:05.07 Mounted lysis Cartridge board#Q05063 @16:57:05.10 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:57:05.42 Preparing to filter cartridge 56 @16:57:05.71 Priming sample loop w/100ml, bypass w/10ml @16:57:05.73 SamplePump.setPosition! 0ml @16:57:05.78 Exhaust.close @16:57:05.86 Bypass.open @16:57:07.30 Intake.open @16:57:08.05 Sea 2.4m deep (18.2psia) @16:57:08.07 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:57:11.16 Vacuum tare is 60% of full scale @16:57:11.33 Pressure.in :default @16:57:11.39 Pressure.hold @16:57:11.61 SamplePump.in :prime @16:57:11.68 SamplePump.seek 10ml,59 seconds @16:57:20.39 Pressure.stop @16:57:20.44 SamplePump.setPosition! 0ml @16:57:20.49 Exhaust.open @16:57:21.94 Bypass.close @16:57:23.48 Pressure.in :default @16:57:23.54 Pressure.hold @16:57:23.67 Sea 1.6m deep (17.0psia) @16:57:23.84 SamplePump.in :prime @16:57:23.91 SamplePump.seek 100ml,5:16 @16:58:54.97 Pressure.stop @16:58:55.04 Bypass.open @16:58:55.73 Intake.close @16:58:57.00 Toroid.in :hold @16:58:57.06 Toroid.seek :crack @16:58:58.63 Toroid.seek :cartridge @16:58:59.76 Toroid.in :default @16:58:59.82 Toroid.seek :clear @16:59:01.83 Pressurizing cartridge to 26psi above 14.7psia @16:59:01.85 SamplePump.setPosition! 0ml @16:59:01.89 Intake.open @16:59:02.53 Bypass.close @16:59:03.23 Exhaust.close @16:59:04.07 Pressure.in :default @16:59:04.13 Pressure.hold @16:59:04.35 SamplePump.in :charge @16:59:04.42 SamplePump.seek 2.3ml,42 seconds @16:59:22.19 Pressure.stop @16:59:22.25 Intake.close @16:59:22.47 Pressurized cartridge with 1.508ml to 26.2psi @16:59:22.49 Isolated @39.5psia (24.8psi) @16:59:22.50 Verifying cartridge holds at least 0.65..0.85 of 39.5psia @16:59:22.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:59:27.12 Cartridge pressure fell 2% to 38.9psia @16:59:27.23 Sampling 1000.0ml in 1:25:20 @16:59:27.25 SamplePump.setPosition! 0ml @16:59:27.30 Exhaust.open @16:59:28.03 Intake.open @16:59:28.91 Pressure.in :default @16:59:28.97 Pressure.hold @16:59:29.12 Sea 4.0m deep (20.6psia) @16:59:29.13 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @16:59:29.29 SamplePump.in :sample @16:59:29.36 SamplePump.seek 1000ml,1:23:51 @16:59:31.97 Cartridge 56 filtering 1/1000ml at 0.42ml/s with 25.8psi vacuum @17:00:31.98 Cartridge 56 filtering 30/1000ml at 0.46ml/s with 23.8psi vacuum @17:01:31.97 Cartridge 56 filtering 57/1000ml at 0.44ml/s with 24.9psi vacuum @17:02:31.97 Cartridge 56 filtering 83/1000ml at 0.42ml/s with 24.9psi vacuum @17:03:31.97 Cartridge 56 filtering 106/1000ml at 0.36ml/s with 22.8psi vacuum @17:04:31.97 Cartridge 56 filtering 128/1000ml at 0.34ml/s with 24.4psi vacuum @17:05:31.98 Cartridge 56 filtering 148/1000ml at 0.33ml/s with 25.1psi vacuum @17:06:32.46 Cartridge 56 filtering 166/1000ml at 0.29ml/s with 22.6psi vacuum 1.8m deep (17.3psia) @17:07:31.97 Cartridge 56 filtering 183/1000ml at 0.26ml/s with 23.3psi vacuum @17:08:31.97 Cartridge 56 filtering 199/1000ml at 0.23ml/s with 23.8psi vacuum @17:09:31.98 Cartridge 56 filtering 213/1000ml at 0.24ml/s with 25.2psi vacuum @17:10:31.97 Cartridge 56 filtering 227/1000ml at 0.22ml/s with 24.0psi vacuum @17:11:31.97 Cartridge 56 filtering 240/1000ml at 0.21ml/s with 24.7psi vacuum @17:12:31.97 Cartridge 56 filtering 252/1000ml at 0.20ml/s with 24.9psi vacuum @17:13:31.99 Cartridge 56 filtering 264/1000ml at 0.20ml/s with 26.1psi vacuum @17:13:32.07 Flow averaged 0.31ml/s over 14:03 @17:13:32.11 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @17:13:32.96 Relieved excess vacuum in .9 seconds @17:13:33.00 SamplePump.seek 1000ml,1:01:50 @17:13:35.15 Flow estimate reduced to 0.25ml/s @17:13:35.19 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @17:13:36.03 Relieved excess vacuum in .9 seconds @17:13:36.07 SamplePump.seek 1000ml,1:01:47 @17:13:38.13 Flow estimate reduced to 0.20ml/s @17:13:38.17 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @17:13:39.02 Relieved excess vacuum in .9 seconds @17:13:39.05 SamplePump.seek 1000ml,1:01:45 @17:13:41.37 Flow estimate reduced to 0.16ml/s @17:13:41.40 Intake.close @17:13:41.45 Exhaust.close @17:13:41.76 Toroid.in :hold @17:13:41.85 Toroid.seek :bypass @17:13:43.51 Toroid.in :default @17:13:43.59 Toroid.seek :clear @17:13:45.21 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @17:13:45.23 Pressure.stop @17:13:45.44 Sampled 265.7ml @17:13:46.06 NoMethodError in PROCESSING -- undefined method `processingOptions' for {:" Type"=>:daSPR_phyto, :State=>:processing, :filtered=>265.7}:Cartridge::State @17:13:46.14 Thread::ChildDied in MAIN -- :PROCESSING @17:13:46.18
Waiting to Resume from Checkpoint ":PROCESSING" @17:47:01.46 Session Begun @17:47:05.80 -> showlog 200 @17:47:52.37 -> backtrace :PROCESSING @17:13:45 NoMethodError: undefined method `processingOptions' for {:" Type"=>:daSPR_phyto, :State=>:processing, :filtered=>265.7}:Cartridge::State @17:48:41.29 -> cart=Cartridge.mounted { Type: :daSPR_phyto, State: :processing, filtered: 265.7} @17:49:17.32 -> Cart.processingOptions {} @17:59:21.94 -> slots {[55,54] => { Type: :daSPR_phyto, State: :dry}, 56 => { Type: :daSPR_phyto, State: :processing, filtered: 265.7}} @18:00:46.56 -> Cartridge.processingMode :paused @18:49:12.25 -> trouble >>> Thread[:MAIN] is suspended <<< @16:56:50 Cartridge group: dry @16:56:55 Selecting Cartridge 56 @17:13:45 :PROCESSING @18:53:19.99 -> progress >>> Thread[:MAIN] is suspended <<< @16:56:50 Cartridge group: dry @16:56:55 Selecting Cartridge 56 @17:13:45 :PROCESSING @18:53:36.93 -> Thread[:PROCESSING] # @18:54:31.64 -> reload Sampler::SPRcontext [/home/esp/esp2/protocol/3G/spr.rb] @19:01:58.99 -> Cmd.state :PROCESS_FAILED @19:04:28.73 -> Cmd.processing # @19:05:23.86 -> Cmd.instance_eval{@state} :PROCESS_FAILED @19:05:34.55 -> Cmd.instance_eval{@state=:PAUSED} :PAUSED @19:05:46.87 -> Cart { Type: :daSPR_phyto, State: :processing, filtered: 265.7} @19:06:10.56 -> Cart.states [:dry, :standard, :wet, :filtering, :paused, :processed, :postprocessing, :postprocessed, :missing, :reserved, :incompatible, :leaky, :leakyToroid, :failedLoading, :failedFiltering, :processing, :failedProcessing, :preserving, :lysing, :analyzing] @19:07:39.25 -> Cart.state :processing @19:07:44.46 -> Cart.state=:paused :paused @19:07:51.37 -> Cart { Type: :daSPR_phyto, State: :paused, filtered: 265.7} @19:08:19.24 -> Cmd.process @19:08:20.80 SPR.command "read_sequences" @19:08:20.87 SPR.command "stats_on" @19:08:21.04 SPR.command "start 0 1" @19:08:21.74 SPR.start /var/log/esp/spr23mar3119h.spr.bz2 @19:08:24.13 Handoff.select :waste @19:08:30.86 Syringe tare is 16% of full scale @19:08:30.89 PinPort.in :locking @19:08:31.00 PinPort.seek :locked @19:08:31.53 Archive.in :fast @19:08:31.58 Lysis2.in :fast @19:08:31.78 Lysis1.seek 1350uL @19:08:31.87 Lysis2.seek :full @19:08:31.92 Archive.seek :full @19:08:33.99 Plunge.seek :reagent @19:08:34.93 Evacuating puck with filter bubble point of 50psi @19:08:34.96 PinPort.seek :locked @19:08:35.10 Handoff.select :waste @19:08:41.72 Air syringe pumping up to 1 strokes to reach 8psi @19:08:41.73 Air stroke #1 of 1 @19:08:42.32 Air.in :pressurize @19:08:42.52 Air.seek :pressurized @19:08:48.32 0.5psi after pressurize @19:08:48.35 Air.in :compress @19:08:48.46 Air.seek :evac @19:08:51.52 1.1psi after compress @19:09:02.15 0.1psi after compress (stable) @19:09:02.16 Air.in :default @19:09:02.27 Air.hold @19:09:02.52 at 0.0psi Opened to waste @19:09:02.53 Handoff.select :blocked @19:09:02.74 Archive syringe pumping up to 8 strokes to reach 50psi @19:09:02.76 Archive stroke #1 of 8 @19:09:02.91 Archive.in :pressurize @19:09:03.02 Archive.seek :pressurized @19:09:15.63 0.3psi after pressurize @19:09:21.23 0.4psi after pressurize (stable) @19:09:21.26 Archive.in :compress @19:09:21.77 Archive.seek :empty @19:09:36.56 11.9psi after compress @19:09:47.15 12.5psi after compress (stable) @19:09:47.18 Archive.in :gulp @19:09:47.29 Archive.seek :topVent @19:09:53.64 13.1psi after gulp @19:09:59.24 13.5psi after gulp (stable) @19:09:59.25 Archive stroke #2 of 8 @19:09:59.39 Archive.in :pressurize @19:09:59.49 Archive.seek :pressurized @19:10:12.03 14.6psi after pressurize @19:10:17.68 14.9psi after pressurize (stable) @19:10:17.71 Archive.in :compress @19:10:17.82 Archive.seek :empty @19:10:32.49 17.8psi after compress @19:11:38.58 41.2psi after compress (stable) @19:11:38.64 Archive.in :gulp @19:11:38.78 Archive.seek :topVent @19:11:45.05 42.0psi after gulp @19:11:50.68 42.3psi after gulp (stable) @19:11:50.69 Archive stroke #3 of 8 @19:11:50.82 Archive.in :pressurize @19:11:50.91 Archive.seek :pressurized @19:12:03.44 42.5psi after pressurize @19:12:09.04 42.6psi after pressurize (stable) @19:12:09.07 Archive.in :compress @19:12:09.19 Archive.seek :empty @19:12:23.88 45.1psi after compress @19:12:32.83 Reached 50.1 of 50.0psi after pumping 5190uL (3.00 strokes) @19:12:32.87 Archive.in :default @19:12:32.99 Archive.hold @19:12:33.08 Handoff.select :waste @19:12:43.81 delay 1:00 @19:13:43.83 Handoff.select :blocked @19:14:06.56 Handoff.select :waste @19:14:07.22 2.1psi at evacEnd @19:14:07.23 Handoff.select :blocked @19:14:07.39 Archive.in :fast @19:14:07.53 Archive.seek :topVent @19:14:21.23 Stable @0.9psi after Handoff blocked @19:14:21.26 Archive syringe pumping up to 8 strokes to reach 31psi @19:14:21.29 Archive stroke #1 of 8 @19:14:21.42 Archive.in :pressurize @19:14:21.51 Archive.seek :pressurized @19:14:34.06 1.9psi after pressurize @19:14:34.08 Archive.in :compress @19:14:34.21 Archive.seek :empty @19:14:48.86 7.7psi after compress @19:15:12.96 15.9psi after compress (stable) @19:15:12.99 Archive.in :gulp @19:15:13.10 Archive.seek :topVent @19:15:19.44 17.0psi after gulp @19:15:25.07 17.5psi after gulp (stable) @19:15:25.09 Archive stroke #2 of 8 @19:15:25.24 Archive.in :pressurize @19:15:25.33 Archive.seek :pressurized @19:15:37.87 18.6psi after pressurize @19:15:37.90 Archive.in :compress @19:15:38.01 Archive.seek :empty @19:15:52.69 24.4psi after compress @19:16:04.35 Reached 31.0 of 30.9psi after pumping 3460uL (2.00 strokes) @19:16:04.37 Archive.in :default @19:16:04.48 Archive.hold @19:16:04.59 Handoff.select :waste @19:16:11.89 delay 1:00 @19:17:11.92 Handoff.select :blocked @19:17:34.69 Handoff.select :waste @19:17:34.84 1.2psi at evacEnd @19:17:34.86 Air.seek :fullVent @19:17:36.33 Archive.in :fast @19:17:36.42 Archive.seek :topVent @19:17:43.61 Seawater evacuated, Pressure above/below filter released. @19:17:43.73 Lysis1.in :slow @19:17:43.83 Lysis1.seek 1050uL @19:18:13.89 delay 5 seconds @19:18:18.91 Handoff.select :blocked @19:18:19.15 Lysis1.seek 450uL @19:19:19.15 delay 3 seconds @19:19:22.25 Air.in mix with 1.40048 parts Lysis1 @19:19:22.98 Air.seek :evac @19:19:23.04 Lysis1.seek 300uL @19:19:38.21 Lysis1.seek :empty @19:20:08.36 Lysis buffer delivered @48.7psi @19:20:08.38 Polling Can every 2:00 @19:20:08.95 PuckHeater.configure :default #ambient 21.9C @19:20:09.03 PuckHeater.seek 79.7C @19:21:06.29 PuckHeater.hold 2:00,79.7C @19:23:05.90 Polling Can every 2:00 @19:23:06.46 PuckHeater.configure :default #ambient 22.2C @19:23:06.56 PuckHeater.hold 30 seconds,78.7C @19:23:36.73 Polling Can every 2:00 @19:23:37.28 PuckHeater.configure :default #ambient 22.2C @19:23:37.36 PuckHeater.hold 30 seconds,78.2C @19:24:07.46 Polling Can every 2:00 @19:24:08.11 PuckHeater.configure :default #ambient 22.2C @19:24:08.22 PuckHeater.hold 10:00,77.6C @19:34:06.15 Lysis complete. Syringe pressure @63.0psi @19:34:06.18 Archive syringe pumping up to 5 strokes to reach 40psi @19:34:06.21 Archive stroke #1 of 5 @19:34:06.34 Archive.in :pressurize @19:34:06.42 Archive.seek :pressurized @19:34:06.68 Reached 63.1 of 40.0psi after pumping 1uL (0.00 strokes) @19:34:06.70 Archive.in :default @19:34:06.85 Archive.hold @19:34:06.95 delay 2 seconds @19:34:08.98 Archive.in :fast @19:34:09.07 Archive.seek :full @19:34:09.33 Lysis1.seek 138uL @19:34:23.24 delay 1 second @19:34:24.27 Archive.seek :full @19:34:24.35 Lysis1.seek :empty @19:34:38.32 Handoff.select :blocked @19:34:38.45 Initial air pressure @62.1psi @19:34:38.53 Lysis1.in :extract @19:34:38.70 Lysis1.seek 600uL,nil @19:34:41.09 Archive syringe pumping up to 6 strokes to reach 25psi @19:34:41.13 Archive stroke #1 of 6 @19:34:41.29 Archive.in :pressurize @19:34:41.40 Archive.seek :pressurized @19:34:53.96 10.7psi after pressurize @19:34:59.64 10.6psi after pressurize (stable) @19:34:59.66 Archive.in :compress @19:34:59.83 Archive.seek :empty @19:35:07.19 Archive.in :default @19:35:07.27 Archive.hold @19:35:07.52 Initial 600uL lysate @17.2psi @19:35:07.53 Handoff.select :blocked @19:35:07.68 Initial air pressure @17.9psi @19:35:07.78 Lysis1.seek 825uL,nil @19:35:09.08 Archive syringe pumping up to 6 strokes to reach 17psi @19:35:09.10 Archive stroke #1 of 6 @19:35:09.26 Archive.in :compress @19:35:09.36 Archive.seek :empty @19:35:12.44 Archive.in :default @19:35:12.51 Archive.hold @19:35:12.84 Recovered 825uL lysate @16.1psi @19:35:12.87 Air.in :halfSpeed @19:35:13.01 Air.seek :fullVent @19:35:15.25 Puck Top Vented, bottom @0.9psi @19:35:15.27 Handoff.select :waste @19:35:15.43 delay 5 seconds @19:35:20.53 Handoff open to waste, bottom @0.2psi @19:35:20.57 Air.in :default @19:35:20.72 Air.seek :evac @19:35:22.18 Lysis1.in :DAmix @19:35:22.33 Lysis2.in :DAmix @19:35:23.11 Lysis1.seek 575uL @19:35:23.14 Lysis2.seek 1480uL @19:35:58.25 delay 5 seconds @19:36:03.28 Handoff.select SPR @19:36:03.43 SPR.command "start 2 14" @19:36:05.01 SPR stabilized @19:36:05.19 delay 10 seconds @19:36:15.21 SPR.command "start 1 0" @19:36:17.86 Waiting up to 4:00 for SPR to enter state 1 @19:38:22.14 SPR.command "hold_on" @19:38:23.53 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @19:38:24.17 Lysis1.seek 275uL @19:38:24.22 Lysis2.seek 1180uL @19:39:06.29 delay 15 seconds @19:39:21.31 SPR.command "hold_off" @19:39:24.18 Waiting up to 38:00 for SPR to enter state 7 @19:57:23.81 SPRsummary:-17RIU,2.1RIU,6.04RIU,9.64RIU,high,high,high after 4120s @20:04:01.03 SPR analysis completed @20:04:07.56 Ended log spr23mar3119h.spr.bz2 @20:04:07.59 SPR.command "stats_off" :PROCESSED @20:17:58.73 -> slots {[55,54] => { Type: :daSPR_phyto, State: :dry}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 265.7, lysed: 825, spr: spr23mar3119h.spr.bz2}} @20:18:23.36 -> ESP.main.exit @20:18:23.48 Handoff.select :close # @20:18:23.73 Session Ended @23:18:15.57PDT02-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @23:18:15.60 >BREAK< ESP3 Gateway v1.42 for cartridge bus @23:18:18.81 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @23:18:19.43 Sea pressure (Abs) tare is 6% of full scale @23:18:22.61 Polling Can every 2:00 @23:18:22.68 Loading /home/esp/esp2/bin/processAll mission @23:18:22.89 Sea pressure (Abs) tare is 6% of full scale @23:18:22.90 Sampling default goal volumes @23:18:22.92 Cartridge group: dry @23:18:23.06 Gate.power :analytic1,:ON,limit to 5000mA @23:18:23.07 Gate.power :analytic2,:ON,limit to 5000mA @23:18:23.08 Gate.power :analytic3,:ON,limit to 5000mA @23:18:23.54 Gate.power :cartridge,:OFF @23:18:23.68 SPR Module 1, FW Rev. B, FPGA Rev. C @23:18:23.86
Archive.configure :default @23:18:24.01 Air.configure :default @23:18:24.13 Lysis1.configure :default @23:18:24.26 Lysis2.configure :default @23:18:24.41 Plunge.configure :default @23:18:24.56 SamplePump.configure :default @23:18:24.73 Toroid.configure :default @23:18:24.79 Toroid.hold @23:18:24.86 Toroid.configure :default @23:18:25.01 Carousel.configure :default @23:18:25.07 Carousel.hold @23:18:25.14 Carousel.configure :default @23:18:25.29 Pressure.configure :default @23:18:25.44 ExtraPump.configure :default @23:18:25.56 PinPort.configure :default @23:18:25.62 All elf controllers running firmware version 4.83 @23:18:26.39 Toroid.seek :clear @23:18:26.47 Carousel.in :default @23:18:26.60 Carousel.home.seek 56 @23:18:26.86 Selecting Cartridge 55 @23:18:27.16 Carousel.seek 55 @23:18:27.68 Lysis1.in :fast @23:18:27.74 Lysis1.seek :locked @23:18:30.35 Plunge.seek :locked @23:18:30.50 Air.in :fast @23:18:30.56 Air.seek :locked @23:18:32.13
Carousel.reconfigure :locked @23:18:32.71 Powering cartridge bus @23:18:36.39 Gate.setIR 1750,5000,0,6 @23:18:36.51 Gate.setIR 0,5000,0,6 @23:18:36.58 PuckHeater.configure :default @23:18:36.66 Gate.power :heat,:ON,limit to 5000mA @23:18:37.40 Mounted lysis Cartridge board#Q05051 @23:18:37.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:18:37.75 Preparing to filter cartridge 55 @23:18:38.04 Priming sample loop w/100ml, bypass w/10ml @23:18:38.06 SamplePump.setPosition! 0ml @23:18:38.10 Exhaust.close @23:18:38.17 Bypass.open @23:18:39.66 Intake.open @23:18:40.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:18:43.00 Vacuum tare is 60% of full scale @23:18:43.15 Pressure.in :default @23:18:43.21 Pressure.hold @23:18:43.44 SamplePump.in :prime @23:18:43.51 SamplePump.seek 10ml,59 seconds @23:18:52.24 Pressure.stop @23:18:52.29 SamplePump.setPosition! 0ml @23:18:52.34 Exhaust.open @23:18:53.75 Bypass.close @23:18:55.32 Pressure.in :default @23:18:55.38 Pressure.hold @23:18:55.67 SamplePump.in :prime @23:18:55.73 SamplePump.seek 100ml,5:16 @23:20:25.85 Pressure.stop @23:20:25.91 Bypass.open @23:20:26.64 Intake.close @23:20:27.45 Toroid.in :hold @23:20:27.51 Toroid.seek :crack @23:20:29.05 Toroid.seek :cartridge @23:20:30.16 Toroid.in :default @23:20:30.22 Toroid.seek :clear @23:20:32.23 Pressurizing cartridge to 26psi above 14.9psia @23:20:32.24 SamplePump.setPosition! 0ml @23:20:32.29 Intake.open @23:20:33.42 Bypass.close @23:20:34.11 Exhaust.close @23:20:34.96 Pressure.in :default @23:20:35.02 Pressure.hold @23:20:35.25 SamplePump.in :charge @23:20:35.32 SamplePump.seek 2.3ml,42 seconds @23:20:50.12 Pressure.stop @23:20:50.18 Intake.close @23:20:50.42 Pressurized cartridge with 1.040ml to 26.1psi @23:20:50.43 Isolated @39.1psia (24.1psi) @23:20:50.45 Verifying cartridge holds at least 0.65..0.85 of 39.1psia @23:20:50.47 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:20:55.06 Cartridge pressure fell 5% to 36.9psia @23:20:55.18 Sampling 1000.0ml in 1:25:20 @23:20:55.20 SamplePump.setPosition! 0ml @23:20:55.24 Exhaust.open @23:20:55.97 Intake.open @23:20:56.83 Pressure.in :default @23:20:56.89 Pressure.hold @23:20:57.03 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @23:20:57.20 SamplePump.in :sample @23:20:57.27 SamplePump.seek 1000ml,1:23:51 @23:20:59.89 Cartridge 55 filtering 1/1000ml at 0.48ml/s with 25.1psi vacuum @23:21:59.90 Cartridge 55 filtering 29/1000ml at 0.44ml/s with 24.9psi vacuum @23:22:59.90 Cartridge 55 filtering 54/1000ml at 0.40ml/s with 23.1psi vacuum 1.6m deep (17.1psia) @23:23:59.90 Cartridge 55 filtering 76/1000ml at 0.34ml/s with 26.0psi vacuum @23:24:59.90 Cartridge 55 filtering 94/1000ml at 0.27ml/s with 23.4psi vacuum @23:25:59.90 Cartridge 55 filtering 110/1000ml at 0.24ml/s with 25.6psi vacuum @23:26:59.90 Cartridge 55 filtering 124/1000ml at 0.23ml/s with 25.1psi vacuum @23:27:59.90 Cartridge 55 filtering 137/1000ml at 0.21ml/s with 23.7psi vacuum @23:28:17.18 Flow averaged 0.32ml/s over 7:20 @23:28:17.23 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @23:28:17.83 Relieved excess vacuum in .7 seconds @23:28:17.86 SamplePump.seek 1000ml,1:12:09 @23:28:40.10 Flow averaged 0.20ml/s over 23 seconds @23:28:40.14 Waiting up to 9 seconds for vacuum to reduce from 26.5 to 18.0psi @23:28:41.49 Relieved excess vacuum in 1.3 seconds @23:28:41.52 SamplePump.seek 1000ml,1:11:47 @23:28:52.92 Flow averaged 0.20ml/s over 12 seconds @23:28:52.96 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @23:28:53.56 Relieved excess vacuum in .7 seconds @23:28:53.59 SamplePump.seek 1000ml,1:11:35 @23:28:58.32 Flow estimate reduced to 0.16ml/s @23:28:58.34 Intake.close @23:28:58.43 Exhaust.close @23:28:58.69 Toroid.in :hold @23:28:58.78 Toroid.seek :bypass @23:28:59.90 Cartridge 55 filtering 148/1000ml at 0.00ml/s with 5.4psi vacuum @23:29:00.42 Toroid.in :default @23:29:00.49 Toroid.seek :clear @23:29:02.11 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @23:29:02.13 Pressure.stop @23:29:02.34 Sampled 148.0ml @23:29:04.00 SPR.command "read_sequences" @23:29:04.79 SPR.command "stats_on" @23:29:04.94 SPR.command "start 0 1" @23:29:05.65 SPR.start /var/log/esp/spr23apr0223h.spr.bz2 @23:29:07.64 Handoff.select :waste @23:29:14.34 Syringe tare is 16% of full scale @23:29:14.37 PinPort.in :locking @23:29:14.43 PinPort.seek :locked @23:29:14.91 Archive.in :fast @23:29:15.02 Lysis2.in :fast @23:29:15.08 Lysis1.seek 1350uL @23:29:15.11 Archive.seek :full @23:29:15.29 Lysis2.seek :full @23:29:17.27 Plunge.seek :reagent @23:29:18.72 Evacuating puck with filter bubble point of 50psi @23:29:18.77 PinPort.seek :locked @23:29:18.85 Handoff.select :waste @23:29:25.46 Air syringe pumping up to 1 strokes to reach 8psi @23:29:25.47 Air stroke #1 of 1 @23:29:25.56 Air.in :pressurize @23:29:25.62 Air.seek :pressurized @23:29:31.37 0.7psi after pressurize @23:29:31.39 Air.in :compress @23:29:31.45 Air.seek :evac @23:29:34.46 0.8psi after compress @23:29:45.07 0.1psi after compress (stable) @23:29:45.09 Air.in :default @23:29:45.15 Air.hold @23:29:45.36 at 0.0psi Opened to waste @23:29:45.37 Handoff.select :blocked @23:29:45.49 Archive syringe pumping up to 8 strokes to reach 50psi @23:29:45.51 Archive stroke #1 of 8 @23:29:46.10 Archive.in :pressurize @23:29:46.17 Archive.seek :pressurized @23:29:58.72 7.7psi after pressurize @23:30:16.81 12.2psi after pressurize (stable) @23:30:16.83 Archive.in :compress @23:30:16.89 Archive.seek :empty @23:30:31.53 14.3psi after compress @23:30:48.13 35.8psi after compress (stable) @23:30:48.15 Archive.in :gulp @23:30:48.21 Archive.seek :topVent @23:30:54.47 36.3psi after gulp @23:31:00.07 36.0psi after gulp (stable) @23:31:00.08 Archive stroke #2 of 8 @23:31:00.17 Archive.in :pressurize @23:31:00.23 Archive.seek :pressurized @23:31:12.75 35.3psi after pressurize @23:31:18.34 35.6psi after pressurize (stable) @23:31:18.36 Archive.in :compress @23:31:18.42 Archive.seek :empty @23:31:31.76 Reached 50.3 of 50.0psi after pumping 3431uL (1.98 strokes) @23:31:31.78 Archive.in :default @23:31:31.84 Archive.hold @23:31:31.98 Handoff.select :waste @23:31:39.70 delay 1:00 @23:32:39.72 Handoff.select :blocked @23:33:16.42 Handoff.select :waste @23:33:16.54 6.6psi at evacEnd @23:33:16.55 Handoff.select :blocked @23:33:16.68 Archive.in :fast @23:33:16.74 Archive.seek :topVent @23:33:31.83 Stable @4.1psi after Handoff blocked @23:33:31.86 Archive syringe pumping up to 8 strokes to reach 34psi @23:33:31.87 Archive stroke #1 of 8 @23:33:31.96 Archive.in :pressurize @23:33:32.08 Archive.seek :pressurized @23:33:44.58 5.7psi after pressurize @23:33:44.60 Archive.in :compress @23:33:44.69 Archive.seek :empty @23:33:59.72 9.4psi after compress @23:34:37.93 23.6psi after compress (stable) @23:34:37.95 Archive.in :gulp @23:34:38.01 Archive.seek :topVent @23:34:44.27 24.6psi after gulp @23:34:49.86 24.9psi after gulp (stable) @23:34:49.87 Archive stroke #2 of 8 @23:34:49.96 Archive.in :pressurize @23:34:50.02 Archive.seek :pressurized @23:35:02.54 25.6psi after pressurize @23:35:02.56 Archive.in :compress @23:35:02.62 Archive.seek :empty @23:35:17.29 27.7psi after compress @23:35:38.91 Reached 34.2 of 34.1psi after pumping 3460uL (2.00 strokes) @23:35:38.93 Archive.in :default @23:35:38.99 Archive.hold @23:35:39.07 Handoff.select :waste @23:35:50.28 delay 1:00 @23:36:50.30 Handoff.select :blocked @23:37:13.01 Handoff.select :waste @23:37:13.13 0.2psi at evacEnd @23:37:13.15 Air.seek :fullVent @23:37:14.59 Archive.in :fast @23:37:14.66 Archive.seek :topVent @23:37:21.80 Seawater evacuated, Pressure above/below filter released. @23:37:21.94 Lysis1.in :slow @23:37:22.01 Lysis1.seek 1050uL @23:37:52.05 delay 5 seconds @23:37:57.07 Handoff.select :blocked @23:37:57.28 Lysis1.seek 450uL @23:38:57.26 delay 3 seconds @23:39:00.36 Air.in mix with 1.40048 parts Lysis1 @23:39:00.97 Air.seek :evac @23:39:01.02 Lysis1.seek 300uL @23:39:16.20 Lysis1.seek :empty @23:39:46.34 Lysis buffer delivered @17.3psi @23:39:46.35 Polling Can every 2:00 @23:39:46.90 PuckHeater.configure :default #ambient 21.0C @23:39:46.95 PuckHeater.seek 79.8C @23:40:43.15 PuckHeater.hold 2:00,79.8C @23:42:43.20 Polling Can every 2:00 @23:42:43.74 PuckHeater.configure :default #ambient 21.3C @23:42:43.80 PuckHeater.hold 30 seconds,78.7C @23:43:13.99 Polling Can every 2:00 @23:43:14.54 PuckHeater.configure :default #ambient 21.4C @23:43:14.60 PuckHeater.hold 30 seconds,78.2C @23:43:44.85 Polling Can every 2:00 @23:43:45.43 PuckHeater.configure :default #ambient 21.4C @23:43:45.49 PuckHeater.hold 10:00,77.7C @23:52:18.59 PuckHeater.configure :default #ambient 22.0C @23:52:18.70 PuckHeater.hold 1:26.92,77.6C @23:53:45.92 Lysis complete. Syringe pressure @19.6psi @23:53:45.94 Archive syringe pumping up to 5 strokes to reach 40psi @23:53:45.95 Archive stroke #1 of 5 @23:53:46.03 Archive.in :pressurize @23:53:46.10 Archive.seek :pressurized @23:53:58.60 19.3psi after pressurize @23:54:04.20 19.2psi after pressurize (stable) @23:54:04.22 Archive.in :compress @23:54:04.28 Archive.seek :empty @23:54:12.41 Reached 40.6 of 40.0psi after pumping 1589uL (0.92 strokes) @23:54:12.43 Archive.in :default @23:54:12.49 Archive.hold @23:54:12.56 delay 2 seconds @23:54:14.59 Archive.in :fast @23:54:14.67 Archive.seek :full @23:54:21.37 Lysis1.seek 138uL @23:54:35.27 delay 1 second @23:54:36.30 Archive.seek :full @23:54:36.34 Lysis1.seek :empty @23:54:50.30 Handoff.select :blocked @23:54:50.42 Initial air pressure @44.1psi @23:54:50.49 Lysis1.in :extract @23:54:50.59 Lysis1.seek 600uL,nil @23:54:53.78 Archive syringe pumping up to 6 strokes to reach 25psi @23:54:53.79 Archive stroke #1 of 6 @23:54:53.88 Archive.in :pressurize @23:54:53.97 Archive.seek :pressurized @23:55:06.51 12.0psi after pressurize @23:55:12.11 11.9psi after pressurize (stable) @23:55:12.13 Archive.in :compress @23:55:12.20 Archive.seek :empty @23:55:19.32 Archive.in :default @23:55:19.40 Archive.hold @23:55:19.57 Initial 600uL lysate @16.5psi @23:55:19.59 Handoff.select :blocked @23:55:19.68 Initial air pressure @16.9psi @23:55:19.76 Lysis1.seek 825uL,nil @23:55:21.03 Archive syringe pumping up to 6 strokes to reach 17psi @23:55:21.06 Archive stroke #1 of 6 @23:55:21.20 Archive.in :compress @23:55:21.26 Archive.seek :empty @23:55:26.37 Archive.in :default @23:55:26.45 Archive.hold @23:55:26.75 Recovered 825uL lysate @17.2psi @23:55:26.77 Air.in :halfSpeed @23:55:26.86 Air.seek :fullVent @23:55:29.08 Puck Top Vented, bottom @13.8psi @23:55:29.10 Handoff.select :waste @23:55:29.21 delay 5 seconds @23:55:34.33 Handoff open to waste, bottom @0.4psi @23:55:34.35 Air.in :default @23:55:34.42 Air.seek :evac @23:55:35.84 Lysis2.in :DAmix @23:55:35.97 Lysis1.in :DAmix @23:55:37.02 Lysis2.seek 1480uL @23:55:37.06 Lysis1.seek 575uL @23:56:12.17 delay 5 seconds @23:56:17.19 Handoff.select SPR @23:56:17.32 SPR.command "start 2 14" @23:56:18.25 SPR stabilized @23:56:18.41 delay 10 seconds @23:56:28.43 SPR.command "start 1 0" @23:56:31.62 Waiting up to 4:00 for SPR to enter state 1 @23:58:35.89 SPR.command "hold_on" @23:58:37.79 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @23:58:38.40 Lysis2.seek 1180uL @23:58:38.45 Lysis1.seek 275uL @23:59:20.52 delay 15 seconds @23:59:35.54 SPR.command "hold_off" @23:59:38.89 Waiting up to 38:00 for SPR to enter state 7 @00:17:37.57PDT03-Apr-23 SPRsummary:-19.8RIU,-2.33RIU,2.93RIU,-6.4RIU,high,high,high after 3076s @00:24:14.79 SPR analysis completed @00:24:20.89 Ended log spr23apr0223h.spr.bz2 @00:24:20.91 SPR.command "stats_off" @00:24:21.21 Handoff.select :close @00:24:21.34
Gate.power :heat,:OFF @00:24:21.35 Gate.power :cartridge,:OFF @00:24:21.43 PinPort.in :unlocking @00:24:21.49 PinPort.seek :clear @00:24:21.96 Carousel.in :default @00:24:22.09 Carousel.home.seek 55 @00:24:22.43 Plunge.seek :locked @00:24:23.31 Archive.in :unlocking @00:24:23.47 Archive.seek :clear @00:24:23.53 Air.in :unlocking @00:24:23.73 Air.seek :clear @00:24:23.88 Lysis1.in :unlocking @00:24:23.98 Lysis1.seek :clear @00:24:24.04 Lysis2.in :unlocking @00:24:24.17 Lysis2.seek :clear @00:24:28.08
Gate.power :motor,:OFF @00:24:28.09 Gate.power :core,:OFF @00:24:28.11 Gate.power :analytic1,:OFF @00:24:28.13 Gate.power :analytic2,:OFF @00:24:28.14 Gate.power :analytic3,:OFF @00:24:28.16 Gate.power :main,:OFF @00:24:28.17 Safely stopped and ready to power off @00:24:28.59 Cartridge::None in MAIN -- Not enough dry cartridges remain @09:47:37.82PDT03-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @09:47:37.85 >BREAK< ESP3 Gateway v1.42 for cartridge bus @09:47:41.16 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @09:47:41.78 Sea pressure (Abs) tare is 6% of full scale @09:47:45.13 Polling Can every 2:00 @09:47:45.19 Loading /home/esp/esp2/bin/processAll mission @09:47:45.41 Sea pressure (Abs) tare is 6% of full scale @09:47:45.42 Sampling default goal volumes @09:47:45.43 Cartridge group: dry @09:47:45.58 Gate.power :analytic1,:ON,limit to 5000mA @09:47:45.59 Gate.power :analytic2,:ON,limit to 5000mA @09:47:45.60 Gate.power :analytic3,:ON,limit to 5000mA @09:47:46.30 Archive.configure :default @09:47:46.46 Air.configure :default @09:47:46.59 Lysis1.configure :default @09:47:46.79 SPR Module 1, FW Rev. B, FPGA Rev. C @09:47:46.82
Lysis2.configure :default @09:47:46.98 Plunge.configure :default @09:47:47.13 SamplePump.configure :default @09:47:47.29 Toroid.configure :default @09:47:47.36 Toroid.hold @09:47:47.43 Toroid.configure :default @09:47:47.59 Carousel.configure :default @09:47:47.66 Carousel.hold @09:47:47.73 Carousel.configure :default @09:47:47.88 Pressure.configure :default @09:47:48.04 ExtraPump.configure :default @09:47:48.17 PinPort.configure :default @09:47:48.23 All elf controllers running firmware version 4.83 @09:47:49.10 Toroid.seek :clear @09:47:49.18 Carousel.in :default @09:47:49.31 Carousel.home.seek 55 @09:47:49.57 Selecting Cartridge 54 @09:47:49.88 Carousel.seek 54 @09:47:50.41 Lysis1.in :fast @09:47:50.47 Lysis1.seek :locked @09:47:53.10 Plunge.seek :locked @09:47:53.26 Air.in :fast @09:47:53.33 Air.seek :locked @09:47:54.90
Carousel.reconfigure :locked @09:47:55.44 Powering cartridge bus @09:47:58.17 Gate.setIR 1750,5000,0,6 @09:47:58.29 Gate.setIR 0,5000,0,6 @09:47:58.35 PuckHeater.configure :default @09:47:58.41 Gate.power :heat,:ON,limit to 5000mA @09:47:59.14 Mounted lysis Cartridge board#Q05042 @09:47:59.17 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:47:59.50 Preparing to filter cartridge 54 @09:47:59.82 Priming sample loop w/100ml, bypass w/10ml @09:47:59.84 SamplePump.setPosition! 0ml @09:47:59.88 Exhaust.close @09:47:59.95 Bypass.open @09:48:01.42 Intake.open @09:48:02.18 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:48:04.76 Vacuum tare is 60% of full scale @09:48:04.93 Pressure.in :default @09:48:04.99 Pressure.hold @09:48:05.21 SamplePump.in :prime @09:48:05.28 SamplePump.seek 10ml,59 seconds @09:48:14.03 Pressure.stop @09:48:14.07 SamplePump.setPosition! 0ml @09:48:14.12 Exhaust.open @09:48:15.53 Bypass.close @09:48:17.07 Pressure.in :default @09:48:17.14 Pressure.hold @09:48:17.44 SamplePump.in :prime @09:48:17.51 SamplePump.seek 100ml,5:16 @09:49:47.75 Pressure.stop @09:49:47.84 Bypass.open @09:49:48.50 Intake.close @09:49:49.26 Toroid.in :hold @09:49:49.32 Toroid.seek :crack @09:49:50.86 Toroid.seek :cartridge @09:49:51.98 Toroid.in :default @09:49:52.04 Toroid.seek :clear @09:49:54.05 Pressurizing cartridge to 26psi above 14.9psia @09:49:54.07 SamplePump.setPosition! 0ml @09:49:54.12 Intake.open @09:49:55.25 Bypass.close @09:49:55.88 Exhaust.close @09:49:56.76 Pressure.in :default @09:49:56.82 Pressure.hold @09:49:57.07 SamplePump.in :charge @09:49:57.14 SamplePump.seek 2.3ml,42 seconds @09:50:11.78 Pressure.stop @09:50:11.85 Intake.close @09:50:12.06 Pressurized cartridge with 1.020ml to 26.3psi @09:50:12.07 Isolated @39.9psia (25.1psi) @09:50:12.09 Verifying cartridge holds at least 0.65..0.85 of 39.9psia @09:50:12.12 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:50:16.84 Sampling 1000.0ml in 1:25:20 @09:50:16.86 SamplePump.setPosition! 0ml @09:50:16.92 Exhaust.open @09:50:17.62 Intake.open @09:50:18.49 Pressure.in :default @09:50:18.55 Pressure.hold @09:50:18.72 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @09:50:18.89 SamplePump.in :sample @09:50:18.96 SamplePump.seek 1000ml,1:23:51 @09:50:21.55 Cartridge 54 filtering 1/1000ml at 0.44ml/s with 24.2psi vacuum @09:51:21.56 Cartridge 54 filtering 28/1000ml at 0.44ml/s with 22.7psi vacuum 1.8m deep (17.3psia) @09:52:21.56 Cartridge 54 filtering 52/1000ml at 0.37ml/s with 25.4psi vacuum @09:53:21.56 Cartridge 54 filtering 74/1000ml at 0.34ml/s with 24.9psi vacuum @09:54:21.56 Cartridge 54 filtering 92/1000ml at 0.28ml/s with 25.0psi vacuum @09:55:21.56 Cartridge 54 filtering 109/1000ml at 0.27ml/s with 24.4psi vacuum @09:56:21.55 Cartridge 54 filtering 123/1000ml at 0.23ml/s with 22.0psi vacuum @09:57:21.56 Cartridge 54 filtering 136/1000ml at 0.20ml/s with 24.8psi vacuum @09:58:21.56 Cartridge 54 filtering 148/1000ml at 0.20ml/s with 26.3psi vacuum @09:58:27.00 Flow averaged 0.31ml/s over 8:09 @09:58:27.04 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @09:58:27.89 Relieved excess vacuum in .9 seconds @09:58:27.92 SamplePump.seek 1000ml,1:11:25 @09:58:43.90 Flow averaged 0.20ml/s over 16 seconds @09:58:43.94 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @09:58:44.80 Relieved excess vacuum in .9 seconds @09:58:44.83 SamplePump.seek 1000ml,1:11:08 @09:58:47.81 Flow estimate reduced to 0.16ml/s @09:58:47.84 Intake.close @09:58:47.91 Exhaust.close @09:58:48.24 Toroid.in :hold @09:58:48.31 Toroid.seek :bypass @09:58:49.96 Toroid.in :default @09:58:50.03 Toroid.seek :clear @09:58:51.65 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:58:51.67 Pressure.stop @09:58:51.89 Sampled 153.1ml @09:58:53.55 SPR.command "read_sequences" @09:58:53.61 SPR.command "stats_on" @09:58:54.52 SPR.command "start 0 1" @09:58:55.22 SPR.start /var/log/esp/spr23apr0309h.spr.bz2 @09:58:57.76 Handoff.select :waste @09:59:04.47 Syringe tare is 16% of full scale @09:59:04.49 PinPort.in :locking @09:59:04.55 PinPort.seek :locked @09:59:05.05 Archive.in :fast @09:59:05.10 Lysis2.in :fast @09:59:05.17 Lysis1.seek 1350uL @09:59:05.43 Archive.seek :full @09:59:05.46 Lysis2.seek :full @09:59:07.42 Plunge.seek :reagent @09:59:08.32 Evacuating puck with filter bubble point of 50psi @09:59:08.34 PinPort.seek :locked @09:59:08.43 Handoff.select :waste @09:59:15.03 Air syringe pumping up to 1 strokes to reach 8psi @09:59:15.05 Air stroke #1 of 1 @09:59:15.13 Air.in :pressurize @09:59:15.20 Air.seek :pressurized @09:59:20.94 0.7psi after pressurize @09:59:20.96 Air.in :compress @09:59:21.02 Air.seek :evac @09:59:24.04 0.7psi after compress @09:59:34.70 0.1psi after compress (stable) @09:59:34.73 Air.in :default @09:59:34.81 Air.hold @09:59:35.23 at 0.1psi Opened to waste @09:59:35.27 Handoff.select :blocked @09:59:35.44 Archive syringe pumping up to 8 strokes to reach 50psi @09:59:35.48 Archive stroke #1 of 8 @09:59:35.67 Archive.in :pressurize @09:59:35.85 Archive.seek :pressurized @09:59:44.56 Session Begun @09:59:47.34 -> showlog @09:59:48.40 9.4psi after pressurize @09:59:58.50 11.0psi after pressurize (stable) @09:59:58.53 Archive.in :compress @09:59:58.67 Archive.seek :empty @10:00:13.35 27.3psi after compress @10:00:28.51 34.3psi after compress (stable) @10:00:28.58 Archive.in :gulp @10:00:28.71 Archive.seek :topVent @10:00:35.00 34.9psi after gulp @10:00:40.60 34.6psi after gulp (stable) @10:00:40.61 Archive stroke #2 of 8 @10:00:40.81 Archive.in :pressurize @10:00:40.98 Archive.seek :pressurized @10:00:53.51 34.0psi after pressurize @10:00:59.11 34.3psi after pressurize (stable) @10:00:59.14 Archive.in :compress @10:00:59.25 Archive.seek :empty @10:01:13.57 Reached 50.3 of 50.0psi after pumping 3451uL (1.99 strokes) @10:01:13.59 Archive.in :default @10:01:13.70 Archive.hold @10:01:13.81 Handoff.select :waste @10:01:30.55 delay 1:00 @10:02:30.58 Handoff.select :blocked @10:02:53.33 Handoff.select :waste @10:02:53.48 2.1psi at evacEnd @10:02:53.50 Handoff.select :blocked @10:02:53.67 Archive.in :fast @10:02:53.77 Archive.seek :topVent @10:03:07.51 Stable @1.8psi after Handoff blocked @10:03:07.53 Archive syringe pumping up to 8 strokes to reach 32psi @10:03:07.57 Archive stroke #1 of 8 @10:03:07.69 Archive.in :pressurize @10:03:07.79 Archive.seek :pressurized @10:03:20.33 2.4psi after pressurize @10:03:20.36 Archive.in :compress @10:03:20.47 Archive.seek :empty @10:03:35.14 6.0psi after compress @10:04:01.24 16.3psi after compress (stable) @10:04:01.27 Archive.in :gulp @10:04:01.42 Archive.seek :topVent @10:04:07.71 16.9psi after gulp @10:04:13.31 17.1psi after gulp (stable) @10:04:13.32 Archive stroke #2 of 8 @10:04:13.46 Archive.in :pressurize @10:04:13.55 Archive.seek :pressurized @10:04:26.10 17.5psi after pressurize @10:04:26.13 Archive.in :compress @10:04:26.24 Archive.seek :empty @10:04:40.91 21.4psi after compress @10:05:06.02 31.3psi after compress (stable) @10:05:06.05 Archive.in :gulp @10:05:06.16 Archive.seek :topVent @10:05:08.88 Reached 31.9 of 31.8psi after pumping 3460uL (2.00 strokes) @10:05:08.90 Archive.in :default @10:05:09.01 Archive.hold @10:05:09.11 Handoff.select :waste @10:05:16.35 delay 1:00 @10:06:16.37 Handoff.select :blocked @10:06:39.11 Handoff.select :waste @10:06:39.26 0.8psi at evacEnd @10:06:39.30 Air.seek :fullVent @10:06:40.79 Archive.in :fast @10:06:40.89 Archive.seek :topVent @10:06:45.61 Seawater evacuated, Pressure above/below filter released. @10:06:45.74 Lysis1.in :slow @10:06:45.84 Lysis1.seek 1050uL @10:07:15.93 delay 5 seconds @10:07:20.95 Handoff.select :blocked @10:07:21.18 Lysis1.seek 450uL @10:08:21.20 delay 3 seconds @10:08:24.32 Air.in mix with 1.40048 parts Lysis1 @10:08:24.95 Air.seek :evac @10:08:25.04 Lysis1.seek 300uL @10:08:40.20 Lysis1.seek :empty @10:09:10.35 Lysis buffer delivered @15.0psi @10:09:10.37 Polling Can every 2:00 @10:09:10.96 PuckHeater.configure :default #ambient 21.0C @10:09:11.04 PuckHeater.seek 79.8C @10:10:08.42 PuckHeater.hold 2:00,79.8C @10:12:08.45 Polling Can every 2:00 @10:12:09.02 PuckHeater.configure :default #ambient 21.4C @10:12:09.10 PuckHeater.hold 30 seconds,78.7C @10:12:39.29 Polling Can every 2:00 @10:12:39.84 PuckHeater.configure :default #ambient 21.5C @10:12:39.92 PuckHeater.hold 30 seconds,78.2C @10:13:10.13 Polling Can every 2:00 @10:13:10.70 PuckHeater.configure :default #ambient 21.6C @10:13:10.79 PuckHeater.hold 10:00,77.7C @10:21:42.05 PuckHeater.configure :default #ambient 22.1C @10:21:42.15 PuckHeater.hold 1:28.76,77.6C @10:23:11.05 Lysis complete. Syringe pressure @17.4psi @10:23:11.08 Archive syringe pumping up to 5 strokes to reach 40psi @10:23:11.11 Archive stroke #1 of 5 @10:23:11.25 Archive.in :pressurize @10:23:11.34 Archive.seek :pressurized @10:23:23.89 17.1psi after pressurize @10:23:29.52 17.1psi after pressurize (stable) @10:23:29.54 Archive.in :compress @10:23:29.65 Archive.seek :empty @10:23:39.19 Reached 42.6 of 40.0psi after pumping 1619uL (0.94 strokes) @10:23:39.20 Archive.in :default @10:23:39.32 Archive.hold @10:23:39.42 delay 2 seconds @10:23:41.44 Archive.in :fast @10:23:41.53 Archive.seek :full @10:23:48.38 Lysis1.seek 138uL @10:24:02.82 delay 1 second @10:24:03.87 Archive.seek :full @10:24:03.94 Lysis1.seek :empty @10:24:17.93 Handoff.select :blocked @10:24:18.08 Initial air pressure @49.6psi @10:24:18.19 Lysis1.in :extract @10:24:18.32 Lysis1.seek 600uL,nil @10:24:20.24 Archive syringe pumping up to 6 strokes to reach 25psi @10:24:20.26 Archive stroke #1 of 6 @10:24:20.46 Archive.in :pressurize @10:24:20.60 Archive.seek :pressurized @10:24:33.17 14.0psi after pressurize @10:27:19.29 15.8psi after pressurize (stable) @10:27:19.33 Archive.in :compress @10:27:19.46 Archive.seek :empty @10:27:31.49 Archive.in :default @10:27:31.57 Archive.hold @10:27:31.83 Initial 600uL lysate @17.5psi @10:27:31.85 Handoff.select :blocked @10:27:31.98 Initial air pressure @18.0psi @10:27:32.08 Lysis1.seek 825uL,nil @10:27:32.78 Archive syringe pumping up to 6 strokes to reach 17psi @10:27:32.79 Archive stroke #1 of 6 @10:27:33.56 Archive.in :compress @10:27:33.67 Archive.seek :empty @10:27:36.38 14.6psi after compress @10:27:43.02 Archive.in :default @10:27:43.10 Archive.hold @10:27:43.39 Recovered 825uL lysate @13.7psi @10:27:43.42 Air.in :halfSpeed @10:27:43.53 Air.seek :fullVent @10:27:45.83 Puck Top Vented, bottom @7.3psi @10:27:45.85 Handoff.select :waste @10:27:46.02 delay 5 seconds @10:27:51.10 Handoff open to waste, bottom @0.2psi @10:27:51.12 Air.in :default @10:27:51.23 Air.seek :evac @10:27:52.72 Lysis2.in :DAmix @10:27:52.89 Lysis1.in :DAmix @10:27:53.42 Lysis2.seek 1480uL @10:27:53.50 Lysis1.seek 575uL @10:28:28.67 delay 5 seconds @10:28:33.71 Handoff.select SPR @10:28:33.86 SPR.command "start 2 14" @10:28:34.97 SPR stabilized @10:28:35.15 delay 10 seconds @10:28:45.17 SPR.command "start 1 0" @10:28:47.85 Waiting up to 4:00 for SPR to enter state 1 @10:30:52.09 SPR.command "hold_on" @10:30:53.52 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:30:54.16 Lysis2.seek 1180uL @10:30:54.23 Lysis1.seek 275uL @10:31:36.33 delay 15 seconds @10:31:51.36 SPR.command "hold_off" @10:31:54.17 Waiting up to 38:00 for SPR to enter state 7 @10:49:53.79 SPRsummary:-7.36RIU,4.23RIU,11.6RIU,11RIU,high,high,high after 3231s @10:56:31.01 SPR analysis completed @10:56:38.48 Ended log spr23apr0309h.spr.bz2 @10:56:38.49 SPR.command "stats_off" @10:56:39.20 Handoff.select :close @10:56:39.36
Gate.power :heat,:OFF @10:56:39.38 Gate.power :cartridge,:OFF @10:56:39.51 PinPort.in :unlocking @10:56:39.60 PinPort.seek :clear @10:56:40.10 Carousel.in :default @10:56:40.26 Carousel.home.seek 54 @10:56:40.62 Plunge.seek :locked @10:56:41.59 Archive.in :unlocking @10:56:41.77 Archive.seek :clear @10:56:41.88 Air.in :unlocking @10:56:42.08 Air.seek :clear @10:56:42.14 Lysis1.in :unlocking @10:56:42.38 Lysis1.seek :clear @10:56:42.54 Lysis2.in :unlocking @10:56:42.67 Lysis2.seek :clear @10:56:46.52
Gate.power :motor,:OFF @10:56:46.54 Gate.power :core,:OFF @10:56:46.58 Gate.power :analytic1,:OFF @10:56:46.61 Gate.power :analytic2,:OFF @10:56:46.70 Gate.power :analytic3,:OFF @10:56:46.73 Gate.power :main,:OFF @10:56:46.77 Safely stopped and ready to power off @10:56:47.28 Cartridge::None in MAIN -- Not enough dry cartridges remain @10:56:47.35 Session Ended @20:04:03.04PDT04-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @20:04:03.06 >BREAK< ESP3 Gateway v1.42 for cartridge bus @20:04:05.47 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @20:04:05.93 Sea pressure (Abs) tare is 6% of full scale @20:04:09.30 Polling Can every 2:00 @20:04:09.33 Loading /home/esp/esp2/bin/processAll mission @20:04:09.50 Sea pressure (Abs) tare is 6% of full scale @20:04:09.51 Sampling maximum of 150.0ml per cartridge @20:04:09.52 Cartridge group: dry @20:04:09.64 Gate.power :analytic1,:ON,limit to 5000mA @20:04:09.65 Gate.power :analytic2,:ON,limit to 5000mA @20:04:09.66 Gate.power :analytic3,:ON,limit to 5000mA @20:04:10.16 SPR Module 1, FW Rev. B, FPGA Rev. C @20:04:10.30
Archive.configure :default @20:04:10.42 Air.configure :default @20:04:10.52 Lysis1.configure :default @20:04:10.62 Lysis2.configure :default @20:04:10.75 Plunge.configure :default @20:04:10.87 SamplePump.configure :default @20:04:10.99 Toroid.configure :default @20:04:11.04 Toroid.hold @20:04:11.09 Toroid.configure :default @20:04:11.21 Carousel.configure :default @20:04:11.26 Carousel.hold @20:04:11.31 Carousel.configure :default @20:04:11.44 Pressure.configure :default @20:04:11.56 ExtraPump.configure :default @20:04:11.66 PinPort.configure :default @20:04:11.71 All elf controllers running firmware version 4.83 @20:04:12.64 Toroid.seek :clear @20:04:12.70 Carousel.in :default @20:04:12.80 Carousel.home.seek 1 @20:04:13.03 Selecting Cartridge 60 @20:04:13.25 Carousel.seek 60 @20:04:25.53 Lysis1.in :fast @20:04:25.58 Lysis1.seek :locked @20:04:28.16 Plunge.seek :locked @20:04:28.27 Air.in :fast @20:04:28.32 Air.seek :locked @20:04:29.88
Carousel.reconfigure :locked @20:04:30.00 Powering cartridge bus @20:04:32.61 Gate.setIR 1750,5000,0,6 @20:04:32.73 Gate.setIR 0,5000,0,6 @20:04:32.78 PuckHeater.configure :default @20:04:32.83 Gate.power :heat,:ON,limit to 5000mA @20:04:33.52 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05040 @20:04:33.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:04:33.79 Preparing to filter cartridge 60 @20:04:34.02 Priming sample loop w/100ml, bypass w/10ml @20:04:34.03 SamplePump.setPosition! 0ml @20:04:34.06 Exhaust.close @20:04:34.12 Bypass.open @20:04:35.55 Intake.open @20:04:36.24 Sea 2.7m deep (18.7psia) @20:04:36.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:04:38.83 Vacuum tare is 60% of full scale @20:04:38.95 Pressure.in :default @20:04:39.00 Pressure.hold @20:04:39.16 SamplePump.in :prime @20:04:39.22 SamplePump.seek 10ml,59 seconds @20:04:47.94 Pressure.stop @20:04:47.98 SamplePump.setPosition! 0ml @20:04:48.02 Exhaust.open @20:04:49.79 Bypass.close @20:04:51.26 Pressure.in :default @20:04:51.31 Pressure.hold @20:04:51.41 Sea 2.7m deep (18.7psia) @20:04:51.54 SamplePump.in :prime @20:04:51.59 SamplePump.seek 100ml,5:16 @20:06:20.70 Pressure.stop @20:06:20.76 Bypass.open @20:06:21.43 Intake.close @20:06:21.62 Toroid.in :hold @20:06:21.67 Toroid.seek :crack @20:06:23.19 Toroid.seek :cartridge @20:06:24.28 Toroid.in :default @20:06:24.33 Toroid.seek :clear @20:06:26.28 Pressurizing cartridge to 26psi above 18.8psia @20:06:26.29 SamplePump.setPosition! 0ml @20:06:26.32 Intake.open @20:06:26.99 Bypass.close @20:06:27.66 Exhaust.close @20:06:28.44 Pressure.in :default @20:06:28.49 Pressure.hold @20:06:28.65 SamplePump.in :charge @20:06:28.70 SamplePump.seek 2.3ml,42 seconds @20:06:45.23 Pressure.stop @20:06:45.27 Intake.close @20:06:46.01 Pressurized cartridge with 1.307ml to 26.0psi @20:06:46.02 Isolated @39.7psia (20.9psi) @20:06:46.03 Verifying cartridge holds at least 0.65..0.85 of 39.7psia @20:06:46.05 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:06:50.63 Cartridge pressure fell 3% to 38.7psia @20:06:50.70 Sampling 150.0ml in 1:25:20 @20:06:50.72 SamplePump.setPosition! 0ml @20:06:50.76 Exhaust.open @20:06:51.48 Intake.open @20:06:52.29 Pressure.in :default @20:06:52.34 Pressure.hold @20:06:52.44 Sea 5.8m deep (23.2psia) @20:06:52.45 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @20:06:52.58 SamplePump.in :sample @20:06:52.63 SamplePump.seek 150ml,13:01 @20:06:55.34 Cartridge 60 filtering 1/150ml at 0.45ml/s with 25.3psi vacuum @20:07:55.35 Cartridge 60 filtering 29/150ml at 0.48ml/s with 23.8psi vacuum 3.7m deep (20.1psia) @20:08:55.35 Cartridge 60 filtering 56/150ml at 0.43ml/s with 23.8psi vacuum 2.7m deep (18.7psia) @20:09:55.35 Cartridge 60 filtering 81/150ml at 0.41ml/s with 25.3psi vacuum @20:10:55.35 Cartridge 60 filtering 105/150ml at 0.39ml/s with 25.6psi vacuum @20:11:55.35 Cartridge 60 filtering 127/150ml at 0.35ml/s with 19.2psi vacuum 4.5m deep (21.4psia) @20:12:55.35 Cartridge 60 filtering 147/150ml at 0.33ml/s with 25.2psi vacuum 2.5m deep (18.4psia) @20:13:03.68 Pressure.stop @20:13:03.78 Intake.close @20:13:03.84 Exhaust.close @20:13:04.02 Toroid.in :hold @20:13:04.07 Toroid.seek :bypass @20:13:05.68 Toroid.in :default @20:13:05.73 Toroid.seek :clear @20:13:07.33 Sampled 150.0ml @20:13:08.83 SPR.command "read_sequences" @20:13:09.17 SPR.command "stats_on" @20:13:09.30 SPR.command "start 0 1" @20:13:10.02 SPR.start /var/log/esp/spr23apr0420h.spr.bz2 @20:13:12.03 Handoff.select :waste @20:13:18.70 Syringe tare is 16% of full scale @20:13:18.72 PinPort.in :locking @20:13:18.76 PinPort.seek :locked @20:13:19.23 Archive.in :fast @20:13:19.29 Lysis2.in :fast @20:13:19.32 Lysis1.seek 1350uL @20:13:19.41 Archive.seek :full @20:13:19.47 Lysis2.seek :full @20:13:21.41 Plunge.seek :reagent @20:13:22.69 Evacuating puck with filter bubble point of 50psi @20:13:22.71 PinPort.seek :locked @20:13:22.77 Handoff.select :waste @20:13:29.36 Air syringe pumping up to 1 strokes to reach 8psi @20:13:29.37 Air stroke #1 of 1 @20:13:29.43 Air.in :pressurize @20:13:29.49 Air.seek :pressurized @20:13:35.27 1.8psi after pressurize @20:13:35.28 Air.in :compress @20:13:35.34 Air.seek :evac @20:13:38.33 0.7psi after compress @20:13:48.91 -0.0psi after compress (stable) @20:13:48.92 Air.in :default @20:13:48.97 Air.hold @20:13:49.52 at -0.0psi Opened to waste @20:13:49.53 Handoff.select :blocked @20:13:49.65 Archive syringe pumping up to 8 strokes to reach 50psi @20:13:49.66 Archive stroke #1 of 8 @20:13:49.73 Archive.in :pressurize @20:13:49.78 Archive.seek :pressurized @20:14:02.27 0.3psi after pressurize @20:14:07.85 0.4psi after pressurize (stable) @20:14:07.86 Archive.in :compress @20:14:07.91 Archive.seek :empty @20:14:22.56 30.5psi after compress @20:14:33.13 31.1psi after compress (stable) @20:14:33.14 Archive.in :gulp @20:14:33.19 Archive.seek :topVent @20:14:39.42 32.4psi after gulp @20:14:44.99 32.7psi after gulp (stable) @20:14:45.00 Archive stroke #2 of 8 @20:14:45.07 Archive.in :pressurize @20:14:45.12 Archive.seek :pressurized @20:14:57.63 32.6psi after pressurize @20:15:03.20 32.9psi after pressurize (stable) @20:15:03.23 Archive.in :compress @20:15:03.27 Archive.seek :empty @20:15:17.89 37.0psi after compress @20:15:28.46 37.2psi after compress (stable) @20:15:28.48 Archive.in :gulp @20:15:28.52 Archive.seek :topVent @20:15:34.76 38.6psi after gulp @20:15:40.33 38.5psi after gulp (stable) @20:15:40.34 Archive stroke #3 of 8 @20:15:40.40 Archive.in :pressurize @20:15:40.45 Archive.seek :pressurized @20:15:52.94 37.7psi after pressurize @20:15:58.51 37.7psi after pressurize (stable) @20:15:58.53 Archive.in :compress @20:15:58.57 Archive.seek :empty @20:16:13.21 41.2psi after compress @20:16:39.79 46.4psi after compress (stable) @20:16:39.80 Archive.in :gulp @20:16:39.85 Archive.seek :topVent @20:16:46.08 48.5psi after gulp @20:16:51.65 48.7psi after gulp (stable) @20:16:51.66 Archive stroke #4 of 8 @20:16:51.73 Archive.in :pressurize @20:16:51.78 Archive.seek :pressurized @20:17:04.31 48.5psi after pressurize @20:17:09.89 48.8psi after pressurize (stable) @20:17:09.93 Archive.in :compress @20:17:09.98 Archive.seek :empty @20:17:20.28 Reached 50.1 of 50.0psi after pumping 6827uL (3.95 strokes) @20:17:20.31 Archive.in :default @20:17:20.37 Archive.hold @20:17:20.42 Handoff.select :waste @20:17:35.60 delay 1:00 @20:18:35.62 Handoff.select :blocked @20:18:58.28 Handoff.select :waste @20:18:58.39 0.1psi at evacEnd @20:18:58.40 Handoff.select :blocked @20:18:58.51 Archive.in :fast @20:18:58.56 Archive.seek :topVent @20:19:11.91 Stable @0.2psi after Handoff blocked @20:19:11.94 Archive syringe pumping up to 8 strokes to reach 30psi @20:19:11.95 Archive stroke #1 of 8 @20:19:12.33 Archive.in :pressurize @20:19:12.44 Archive.seek :pressurized @20:19:24.93 0.3psi after pressurize @20:19:24.95 Archive.in :compress @20:19:25.00 Archive.seek :empty @20:19:39.61 6.4psi after compress @20:20:07.19 14.5psi after compress (stable) @20:20:07.20 Archive.in :gulp @20:20:07.25 Archive.seek :topVent @20:20:13.48 14.9psi after gulp @20:20:19.06 14.9psi after gulp (stable) @20:20:19.08 Archive stroke #2 of 8 @20:20:19.16 Archive.in :pressurize @20:20:19.21 Archive.seek :pressurized @20:20:31.69 15.0psi after pressurize @20:20:31.71 Archive.in :compress @20:20:31.76 Archive.seek :empty @20:20:46.38 21.8psi after compress @20:21:06.47 Reached 30.2 of 30.2psi after pumping 3460uL (2.00 strokes) @20:21:06.49 Archive.in :default @20:21:06.54 Archive.hold @20:21:06.59 Handoff.select :waste @20:21:15.26 delay 1:00 @20:22:15.27 Handoff.select :blocked @20:22:38.36 Handoff.select :waste @20:22:38.47 0.1psi at evacEnd @20:22:38.49 Air.seek :fullVent @20:22:39.89 Archive.in :fast @20:22:39.94 Archive.seek :topVent @20:22:47.07 Seawater evacuated, Pressure above/below filter released. @20:22:47.14 Lysis1.in :slow @20:22:47.19 Lysis1.seek 1050uL @20:23:17.24 delay 5 seconds @20:23:22.25 Handoff.select :blocked @20:23:22.42 Lysis1.seek 450uL @20:24:22.43 delay 3 seconds @20:24:25.51 Air.in mix with 1.40048 parts Lysis1 @20:24:25.96 Air.seek :evac @20:24:26.01 Lysis1.seek 300uL @20:24:41.12 Lysis1.seek :empty @20:25:11.22 Lysis buffer delivered @19.5psi @20:25:11.24 Polling Can every 2:00 @20:25:11.77 PuckHeater.configure :default #ambient 19.1C @20:25:11.81 PuckHeater.seek 79.9C @20:26:11.83 PuckHeater.hold 2:00,79.9C @20:28:11.76 Polling Can every 2:00 @20:28:12.29 PuckHeater.configure :default #ambient 19.2C @20:28:12.33 PuckHeater.hold 30 seconds,78.8C @20:28:42.48 Polling Can every 2:00 @20:28:43.02 PuckHeater.hold 30 seconds,78.3C @20:29:13.17 Polling Can every 2:00 @20:29:13.70 PuckHeater.configure :default #ambient 19.2C @20:29:13.75 PuckHeater.hold 10:00,77.8C @20:39:13.17 Lysis complete. Syringe pressure @23.0psi @20:39:13.19 Archive syringe pumping up to 5 strokes to reach 40psi @20:39:13.20 Archive stroke #1 of 5 @20:39:13.27 Archive.in :pressurize @20:39:13.32 Archive.seek :pressurized @20:39:25.80 22.6psi after pressurize @20:39:31.37 22.6psi after pressurize (stable) @20:39:31.38 Archive.in :compress @20:39:31.43 Archive.seek :empty @20:39:34.71 Reached 40.8 of 40.0psi after pumping 1485uL (0.86 strokes) @20:39:34.72 Archive.in :default @20:39:34.77 Archive.hold @20:39:34.82 delay 2 seconds @20:39:36.85 Archive.in :fast @20:39:36.90 Archive.seek :full @20:39:43.20 Lysis1.seek 138uL @20:39:57.07 delay 1 second @20:39:58.09 Archive.seek :full @20:39:58.13 Lysis1.seek :empty @20:40:12.04 Handoff.select :blocked @20:40:12.12 Initial air pressure @39.5psi @20:40:12.18 Lysis1.in :extract @20:40:12.25 Lysis1.seek 600uL,nil @20:40:13.38 Archive syringe pumping up to 6 strokes to reach 25psi @20:40:13.39 Archive stroke #1 of 6 @20:40:13.46 Archive.in :pressurize @20:40:13.51 Archive.seek :pressurized @20:40:26.02 15.8psi after pressurize @20:40:41.19 Archive.in :default @20:40:41.24 Archive.hold @20:40:41.35 Initial 600uL lysate @16.7psi @20:40:41.37 Handoff.select :blocked @20:40:41.44 Initial air pressure @16.8psi @20:40:41.48 Lysis1.seek 825uL,nil @20:40:42.16 Archive syringe pumping up to 6 strokes to reach 17psi @20:40:42.18 Archive stroke #1 of 6 @20:40:42.24 Archive.in :compress @20:40:42.29 Archive.seek :empty @20:40:46.74 Reached 17.1 of 17.0psi after pumping 1509uL (0.87 strokes) @20:40:47.16 Archive.in :default @20:40:47.31 Archive.hold @20:40:47.59 Archive syringe pumping up to 6 strokes to reach 17psi @20:40:47.60 Archive stroke #1 of 6 @20:40:47.67 Archive.in :compress @20:40:47.77 Archive.seek :empty @20:40:48.33 Reached 17.1 of 17.0psi after pumping 1518uL (0.88 strokes) @20:40:48.35 Archive.in :default @20:40:48.41 Archive.hold @20:40:49.14 Archive syringe pumping up to 6 strokes to reach 17psi @20:40:49.15 Archive stroke #1 of 6 @20:40:49.27 Archive.in :compress @20:40:49.32 Archive.seek :empty @20:40:55.40 Archive.in :default @20:40:55.46 Archive.hold @20:40:55.68 Recovered 825uL lysate @15.9psi @20:40:55.70 Air.in :halfSpeed @20:40:55.75 Air.seek :fullVent @20:40:57.93 Puck Top Vented, bottom @1.8psi @20:40:57.94 Handoff.select :waste @20:40:58.05 delay 5 seconds @20:41:03.11 Handoff open to waste, bottom @0.2psi @20:41:03.13 Air.in :default @20:41:03.18 Air.seek :evac @20:41:04.58 Lysis2.in :DAmix @20:41:04.68 Lysis1.in :DAmix @20:41:05.22 Lysis2.seek 1480uL @20:41:05.27 Lysis1.seek 575uL @20:41:40.33 delay 5 seconds @20:41:45.34 Handoff.select SPR @20:41:45.48 SPR.command "start 2 14" @20:41:46.82 SPR stabilized @20:41:46.93 delay 10 seconds @20:41:56.95 SPR.command "start 1 0" @20:42:00.18 Waiting up to 4:00 for SPR to enter state 1 @20:44:04.43 SPR.command "hold_on" @20:44:05.86 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @20:44:06.34 Lysis2.seek 1180uL @20:44:06.39 Lysis1.seek 275uL @20:44:48.42 delay 15 seconds @20:45:03.43 SPR.command "hold_off" @20:45:06.50 Waiting up to 38:00 for SPR to enter state 7 @21:03:06.09 SPRsummary:-67RIU,-47.4RIU,-46.8RIU,-36RIU,high,high,high after 3165s @21:09:43.32 SPR analysis completed @21:09:48.58 Ended log spr23apr0420h.spr.bz2 @21:09:48.60 SPR.command "stats_off" @21:09:48.83 Handoff.select :close @21:09:48.95
Gate.power :heat,:OFF @21:09:48.96 Gate.power :cartridge,:OFF @21:09:49.03 PinPort.in :unlocking @21:09:49.07 PinPort.seek :clear @21:09:49.53 Carousel.in :default @21:09:49.63 Carousel.home.seek 60 @21:09:49.90 Plunge.seek :locked @21:09:50.76 Archive.in :unlocking @21:09:50.88 Archive.seek :clear @21:09:50.94 Air.in :unlocking @21:09:51.07 Air.seek :clear @21:09:51.15 Lysis1.in :unlocking @21:09:51.25 Lysis1.seek :clear @21:09:51.30 Lysis2.in :unlocking @21:09:51.39 Lysis2.seek :clear @21:09:55.32
Gate.power :motor,:OFF @21:09:55.33 Gate.power :core,:OFF @21:09:55.34 Gate.power :analytic1,:OFF @21:09:55.35 Gate.power :analytic2,:OFF @21:09:55.36 Gate.power :analytic3,:OFF @21:09:55.37 Gate.power :main,:OFF @21:09:55.38 Safely stopped and ready to power off @21:09:59.15 Gate.power :main,:ON,limit to 5000mA @21:09:59.16 Gate.power :analytic1,:ON,limit to 5000mA @21:09:59.17 Gate.power :analytic2,:ON,limit to 5000mA @21:09:59.18 Gate.power :analytic3,:ON,limit to 5000mA @21:09:59.23 Powering core bus @21:10:01.59 SPR Module 1, FW Rev. B, FPGA Rev. C @21:10:02.01
Archive.configure :default @21:10:02.08 Air.configure :default @21:10:02.13 Lysis1.configure :default @21:10:02.18 Lysis2.configure :default @21:10:02.27 Plunge.configure :default @21:10:02.34 SamplePump.configure :default @21:10:02.83 Toroid.configure :default @21:10:02.90 Carousel.configure :default @21:10:02.97 Pressure.configure :default @21:10:03.04 ExtraPump.configure :default @21:10:03.09 PinPort.configure :default @21:10:03.14 All elf controllers running firmware version 4.83 @21:10:03.16 Gate.power :motor,:ON,limit to 5000mA @21:10:03.50 Archive.home.jog 0 @21:10:03.61 Air.home.jog 0 @21:10:03.67 Lysis1.home.jog 0 @21:10:03.74 Lysis2.home.jog 0 @21:10:03.89 Plunge.home.jog 0 @21:10:03.97 Toroid.home.jog 0 @21:10:04.07 Carousel.home.jog 0 @21:10:04.20 PinPort.home.jog 0 @21:10:04.76 Archive.in :homing @21:10:04.97 Archive.jog 1385 @21:10:05.01 Toroid.in :homing @21:10:05.04 Plunge.in :homing @21:10:05.07 Lysis2.in :homing @21:10:05.10 Lysis1.in :homing @21:10:05.13 Air.in :homing @21:10:05.16 PinPort.in :homing @21:10:05.38 Toroid.jog 611 @21:10:05.43 Lysis2.jog 1394 @21:10:05.46 Plunge.jog 1548 @21:10:05.50 Air.jog 1383 @21:10:05.54 PinPort.jog -106 @21:10:05.56 Lysis1.jog 1393 @21:10:05.87 PinPort.setRawPosition! 0 @21:10:05.91 PinPort.seek :home @21:10:05.97 PinPort.in :default @21:10:06.33 Archive.setRawPosition! 50 @21:10:06.38 Archive.seek :home @21:10:06.63 Archive.in :default @21:10:06.72 Lysis2.setRawPosition! 50 @21:10:06.76 Lysis2.seek :home @21:10:06.85 Air.setRawPosition! 50 @21:10:06.92 Lysis1.setRawPosition! 50 @21:10:06.94 Plunge.setRawPosition! 50 @21:10:06.97 Air.seek :home @21:10:07.02 Lysis1.seek :home @21:10:07.08 Plunge.seek :home @21:10:07.22 Lysis2.in :default @21:10:07.32 Air.in :default @21:10:07.39 Lysis1.in :default @21:10:07.46 Plunge.in :default @21:10:07.54 Toroid.setRawPosition! 0 @21:10:07.59 Toroid.seek :home @21:10:07.66 Toroid.in :default @21:10:07.82
Toroid.seek :clear @21:10:09.42 Carousel.in :default @21:10:09.53 Carousel.home.seek 60 @21:10:09.75 Selecting Cartridge 59 @21:10:10.33 Carousel.seek 59 @21:10:10.82 Lysis1.in :fast @21:10:10.87 Lysis1.seek :locked @21:10:13.45 Plunge.seek :locked @21:10:13.57 Air.in :fast @21:10:13.62 Air.seek :locked @21:10:15.18
Carousel.reconfigure :locked @21:10:15.31 Powering cartridge bus @21:10:17.92 Gate.setIR 1750,5000,0,6 @21:10:18.03 Gate.setIR 0,5000,0,6 @21:10:18.08 PuckHeater.configure :default #ambient 19.2C @21:10:18.13 Gate.power :heat,:ON,limit to 5000mA @21:10:18.76 Mounted lysis Cartridge board#Q05058 @21:10:18.78 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:10:19.01 Preparing to filter cartridge 59 @21:10:19.24 Priming sample loop w/100ml, bypass w/10ml @21:10:19.25 SamplePump.setPosition! 0ml @21:10:19.28 Bypass.open @21:10:20.72 Intake.open @21:10:21.42 Sea 2.5m deep (18.4psia) @21:10:21.43 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @21:10:24.01 Vacuum tare is 60% of full scale @21:10:24.12 Pressure.in :default @21:10:24.17 Pressure.hold @21:10:24.34 SamplePump.in :prime @21:10:24.39 SamplePump.seek 10ml,59 seconds @21:10:33.12 Pressure.stop @21:10:33.16 SamplePump.setPosition! 0ml @21:10:33.20 Exhaust.open @21:10:34.60 Bypass.close @21:10:36.09 Pressure.in :default @21:10:36.14 Pressure.hold @21:10:36.24 Sea 2.4m deep (18.2psia) @21:10:36.36 SamplePump.in :prime @21:10:36.42 SamplePump.seek 100ml,5:16 @21:12:06.54 Pressure.stop @21:12:06.60 Bypass.open @21:12:07.29 Intake.close @21:12:08.42 Toroid.in :hold @21:12:08.47 Toroid.seek :crack @21:12:09.99 Toroid.seek :cartridge @21:12:11.08 Toroid.in :default @21:12:11.13 Toroid.seek :clear @21:12:13.07 Pressurizing cartridge to 26psi above 18.5psia @21:12:13.08 SamplePump.setPosition! 0ml @21:12:13.12 Intake.open @21:12:13.76 Bypass.close @21:12:14.43 Exhaust.close @21:12:15.21 Pressure.in :default @21:12:15.25 Pressure.hold @21:12:15.43 SamplePump.in :charge @21:12:15.48 SamplePump.seek 2.3ml,42 seconds @21:12:29.32 Pressure.stop @21:12:29.37 Intake.close @21:12:29.56 Pressurized cartridge with 0.915ml to 26.7psi @21:12:29.58 Isolated @43.1psia (24.6psi) @21:12:29.59 Verifying cartridge holds at least 0.65..0.85 of 43.1psia @21:12:29.61 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @21:12:34.24 Sampling 150.0ml in 1:25:20 @21:12:34.26 SamplePump.setPosition! 0ml @21:12:34.30 Exhaust.open @21:12:35.01 Intake.open @21:12:35.26 Pressure.in :default @21:12:35.31 Pressure.hold @21:12:35.41 Sea 5.1m deep (22.2psia) @21:12:35.42 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @21:12:35.54 SamplePump.in :sample @21:12:35.60 SamplePump.seek 150ml,13:01 @21:12:38.30 Cartridge 59 filtering 1/150ml at 0.45ml/s with 25.8psi vacuum @21:13:38.31 Cartridge 59 filtering 28/150ml at 0.43ml/s with 24.2psi vacuum @21:14:38.31 Cartridge 59 filtering 53/150ml at 0.41ml/s with 25.0psi vacuum @21:15:38.31 Cartridge 59 filtering 76/150ml at 0.37ml/s with 25.7psi vacuum 2.8m deep (18.8psia) @21:16:38.32 Cartridge 59 filtering 98/150ml at 0.34ml/s with 23.7psi vacuum 1.9m deep (17.5psia) @21:17:38.32 Cartridge 59 filtering 117/150ml at 0.28ml/s with 24.2psi vacuum 1.9m deep (17.5psia) @21:18:38.64 Cartridge 59 filtering 134/150ml at 0.28ml/s with 25.3psi vacuum 2.2m deep (17.9psia) @21:19:38.32 Cartridge 59 filtering 149/150ml at 0.25ml/s with 20.8psi vacuum 3.6m deep (20.0psia) @21:19:42.08 Pressure.stop @21:19:42.18 Intake.close @21:19:42.22 Exhaust.close @21:19:42.41 Toroid.in :hold @21:19:42.47 Toroid.seek :bypass @21:19:44.09 Toroid.in :default @21:19:44.14 Toroid.seek :clear @21:19:45.74 Sampled 150.0ml @21:19:47.24 SPR.command "read_sequences" @21:19:47.89 SPR.command "stats_on" @21:19:48.01 SPR.command "start 0 1" @21:19:48.73 SPR.start /var/log/esp/spr23apr0421h.spr.bz2 @21:19:50.74 Handoff.select :waste @21:19:57.41 Syringe tare is 16% of full scale @21:19:57.43 PinPort.in :locking @21:19:57.48 PinPort.seek :locked @21:19:57.94 Archive.in :fast @21:19:57.99 Lysis2.in :fast @21:19:58.03 Lysis1.seek 1350uL @21:19:58.14 Archive.seek :full @21:19:58.19 Lysis2.seek :full @21:20:00.13 Plunge.seek :reagent @21:20:01.00 Evacuating puck with filter bubble point of 50psi @21:20:01.03 PinPort.seek :locked @21:20:01.10 Handoff.select :waste @21:20:07.68 Air syringe pumping up to 1 strokes to reach 8psi @21:20:07.69 Air stroke #1 of 1 @21:20:07.76 Air.in :pressurize @21:20:07.81 Air.seek :pressurized @21:20:13.53 0.0psi after pressurize @21:20:13.55 Air.in :compress @21:20:13.60 Air.seek :evac @21:20:16.59 0.8psi after compress @21:20:27.18 0.1psi after compress (stable) @21:20:27.21 Air.in :default @21:20:27.27 Air.hold @21:20:27.42 at 0.0psi Opened to waste @21:20:27.44 Handoff.select :blocked @21:20:27.55 Archive syringe pumping up to 8 strokes to reach 50psi @21:20:27.56 Archive stroke #1 of 8 @21:20:27.62 Archive.in :pressurize @21:20:28.04 Archive.seek :pressurized @21:20:40.56 8.0psi after pressurize @21:20:54.13 10.6psi after pressurize (stable) @21:20:54.14 Archive.in :compress @21:20:54.19 Archive.seek :empty @21:21:08.84 27.4psi after compress @21:21:24.91 34.2psi after compress (stable) @21:21:24.93 Archive.in :gulp @21:21:24.98 Archive.seek :topVent @21:21:31.21 34.4psi after gulp @21:21:36.78 34.4psi after gulp (stable) @21:21:36.79 Archive stroke #2 of 8 @21:21:37.26 Archive.in :pressurize @21:21:37.32 Archive.seek :pressurized @21:21:49.81 33.9psi after pressurize @21:21:55.38 34.1psi after pressurize (stable) @21:21:55.39 Archive.in :compress @21:21:55.44 Archive.seek :empty @21:22:09.40 Reached 50.3 of 50.0psi after pumping 3445uL (1.99 strokes) @21:22:09.41 Archive.in :default @21:22:09.46 Archive.hold @21:22:09.52 Handoff.select :waste @21:22:24.69 delay 1:00 @21:23:24.71 Handoff.select :blocked @21:23:47.38 Handoff.select :waste @21:23:47.48 2.2psi at evacEnd @21:23:47.49 Handoff.select :blocked @21:23:47.60 Archive.in :fast @21:23:47.66 Archive.seek :topVent @21:24:01.43 Stable @1.4psi after Handoff blocked @21:24:01.45 Archive syringe pumping up to 8 strokes to reach 31psi @21:24:01.46 Archive stroke #1 of 8 @21:24:01.52 Archive.in :pressurize @21:24:01.57 Archive.seek :pressurized @21:24:14.06 2.1psi after pressurize @21:24:14.07 Archive.in :compress @21:24:14.12 Archive.seek :empty @21:24:28.77 6.5psi after compress @21:24:56.85 14.9psi after compress (stable) @21:24:56.86 Archive.in :gulp @21:24:56.91 Archive.seek :topVent @21:25:03.15 15.8psi after gulp @21:25:08.73 16.2psi after gulp (stable) @21:25:08.74 Archive stroke #2 of 8 @21:25:08.81 Archive.in :pressurize @21:25:08.86 Archive.seek :pressurized @21:25:21.37 17.2psi after pressurize @21:25:21.39 Archive.in :compress @21:25:21.44 Archive.seek :empty @21:25:36.06 21.7psi after compress @21:26:02.14 30.3psi after compress (stable) @21:26:02.15 Archive.in :gulp @21:26:02.20 Archive.seek :topVent @21:26:08.43 31.2psi after gulp @21:26:10.64 Reached 31.4 of 31.4psi after pumping 3460uL (2.00 strokes) @21:26:10.65 Archive.in :default @21:26:10.70 Archive.hold @21:26:10.75 Handoff.select :waste @21:26:17.92 delay 1:00 @21:27:17.94 Handoff.select :blocked @21:27:40.61 Handoff.select :waste @21:27:40.71 1.2psi at evacEnd @21:27:40.73 Air.seek :fullVent @21:27:42.13 Archive.in :fast @21:27:42.18 Archive.seek :topVent @21:27:42.25 Seawater evacuated, Pressure above/below filter released. @21:27:42.33 Lysis1.in :slow @21:27:42.39 Lysis1.seek 1050uL @21:28:12.42 delay 5 seconds @21:28:17.43 Handoff.select :blocked @21:28:17.60 Lysis1.seek 450uL @21:29:17.61 delay 3 seconds @21:29:20.69 Air.in mix with 1.40048 parts Lysis1 @21:29:21.19 Air.seek :evac @21:29:21.24 Lysis1.seek 300uL @21:29:36.33 Lysis1.seek :empty @21:30:06.44 Lysis buffer delivered @30.3psi @21:30:06.45 Polling Can every 2:00 @21:30:06.98 PuckHeater.configure :default #ambient 20.0C @21:30:07.02 PuckHeater.seek 79.9C @21:31:08.39 PuckHeater.hold 2:00,79.9C @21:33:08.82 Polling Can every 2:00 @21:33:09.36 PuckHeater.configure :default #ambient 20.1C @21:33:09.40 PuckHeater.hold 30 seconds,78.8C @21:33:39.58 Polling Can every 2:00 @21:33:40.11 PuckHeater.configure :default #ambient 20.1C @21:33:40.15 PuckHeater.hold 30 seconds,78.3C @21:34:10.34 Polling Can every 2:00 @21:34:10.88 PuckHeater.configure :default #ambient 20.1C @21:34:10.92 PuckHeater.hold 10:00,77.7C @21:44:11.02 Lysis complete. Syringe pressure @34.4psi @21:44:11.04 Archive syringe pumping up to 5 strokes to reach 40psi @21:44:11.05 Archive stroke #1 of 5 @21:44:11.11 Archive.in :pressurize @21:44:11.16 Archive.seek :pressurized @21:44:23.64 33.6psi after pressurize @21:44:29.21 33.8psi after pressurize (stable) @21:44:29.22 Archive.in :compress @21:44:29.27 Archive.seek :empty @21:44:34.12 Reached 41.1 of 40.0psi after pumping 1519uL (0.88 strokes) @21:44:34.14 Archive.in :default @21:44:34.18 Archive.hold @21:44:34.24 delay 2 seconds @21:44:36.27 Archive.in :fast @21:44:36.32 Archive.seek :full @21:44:42.73 Lysis1.seek 138uL @21:44:56.59 delay 1 second @21:44:57.62 Archive.seek :full @21:44:57.66 Lysis1.seek :empty @21:45:11.54 Handoff.select :blocked @21:45:11.62 Initial air pressure @55.5psi @21:45:11.67 Lysis1.in :extract @21:45:11.77 Lysis1.seek 600uL,nil @21:45:13.42 Archive syringe pumping up to 6 strokes to reach 25psi @21:45:13.43 Archive stroke #1 of 6 @21:45:13.50 Archive.in :pressurize @21:45:13.57 Archive.seek :pressurized @21:45:26.06 15.5psi after pressurize @21:46:06.17 Archive.in :default @21:46:06.22 Archive.hold @21:46:06.34 Initial 600uL lysate @16.2psi @21:46:06.35 Handoff.select :blocked @21:46:06.42 Initial air pressure @16.3psi @21:46:06.47 Lysis1.seek 825uL,nil @21:46:07.61 Archive syringe pumping up to 6 strokes to reach 17psi @21:46:07.62 Archive stroke #1 of 6 @21:46:07.69 Archive.in :compress @21:46:07.76 Archive.seek :empty @21:46:17.20 Reached 17.1 of 17.0psi after pumping 1617uL (0.93 strokes) @21:46:17.21 Archive.in :default @21:46:17.27 Archive.hold @21:46:17.46 Archive syringe pumping up to 6 strokes to reach 17psi @21:46:17.47 Archive stroke #1 of 6 @21:46:17.55 Archive.in :compress @21:46:17.61 Archive.seek :empty @21:46:22.06 Reached 15.8 of 17.0psi after pumping 1701uL (0.98 strokes) @21:46:22.10 Archive.in :default @21:46:22.21 Archive.hold @21:46:22.38 Archive syringe pumping up to 6 strokes to reach 17psi @21:46:22.40 Archive stroke #1 of 6 @21:46:22.46 Archive.in :compress @21:46:22.52 Archive.seek :empty @21:46:24.05 14.1psi after compress @21:46:25.19 Archive.in :default @21:46:25.24 Archive.hold @21:46:25.43 Recovered 825uL lysate @14.6psi @21:46:25.45 Air.in :halfSpeed @21:46:25.50 Air.seek :fullVent @21:46:27.69 Puck Top Vented, bottom @2.2psi @21:46:27.70 Handoff.select :waste @21:46:27.81 delay 5 seconds @21:46:32.88 Handoff open to waste, bottom @-0.0psi @21:46:32.89 Air.in :default @21:46:32.94 Air.seek :evac @21:46:34.34 Lysis2.in :DAmix @21:46:34.46 Lysis1.in :DAmix @21:46:34.95 Lysis2.seek 1480uL @21:46:35.00 Lysis1.seek 575uL @21:47:10.06 delay 5 seconds @21:47:15.08 Handoff.select SPR @21:47:15.21 SPR.command "start 2 14" @21:47:16.48 SPR stabilized @21:47:16.60 delay 10 seconds @21:47:26.61 SPR.command "start 1 0" @21:47:29.84 Waiting up to 4:00 for SPR to enter state 1 @21:49:34.14 SPR.command "hold_on" @21:49:35.52 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:49:36.01 Lysis2.seek 1180uL @21:49:36.05 Lysis1.seek 275uL @21:50:18.06 delay 15 seconds @21:50:33.07 SPR.command "hold_off" @21:50:36.18 Waiting up to 38:00 for SPR to enter state 7 @22:08:35.79 SPRsummary:18.8RIU,53.2RIU,62.8RIU,88.4RIU,182ng/L,148ng/L,164ng/L after 3092s @22:15:13.00 SPR analysis completed @22:15:18.46 Ended log spr23apr0421h.spr.bz2 @22:15:18.47 SPR.command "stats_off" @22:15:19.27 Handoff.select :close @22:15:19.39
Gate.power :heat,:OFF @22:15:19.40 Gate.power :cartridge,:OFF @22:15:19.46 PinPort.in :unlocking @22:15:19.51 PinPort.seek :clear @22:15:19.97 Carousel.in :default @22:15:20.06 Carousel.home.seek 59 @22:15:20.34 Plunge.seek :locked @22:15:21.19 Archive.in :unlocking @22:15:21.31 Archive.seek :clear @22:15:21.37 Air.in :unlocking @22:15:21.51 Air.seek :clear @22:15:21.54 Lysis1.in :unlocking @22:15:21.70 Lysis1.seek :clear @22:15:21.74 Lysis2.in :unlocking @22:15:21.84 Lysis2.seek :clear @22:15:25.85
Gate.power :motor,:OFF @22:15:25.87 Gate.power :core,:OFF @22:15:25.88 Gate.power :analytic1,:OFF @22:15:25.89 Gate.power :analytic2,:OFF @22:15:25.90 Gate.power :analytic3,:OFF @22:15:25.91 Gate.power :main,:OFF Safely stopped and ready to power off @22:15:29.68 Gate.power :main,:ON,limit to 5000mA @22:15:29.70 Gate.power :analytic1,:ON,limit to 5000mA @22:15:29.71 Gate.power :analytic2,:ON,limit to 5000mA @22:15:29.72 Gate.power :analytic3,:ON,limit to 5000mA @22:15:29.77 Powering core bus @22:15:32.13 SPR Module 1, FW Rev. B, FPGA Rev. C @22:15:32.47
Archive.configure :default @22:15:32.54 Air.configure :default @22:15:32.59 Lysis1.configure :default @22:15:32.64 Lysis2.configure :default @22:15:32.72 Plunge.configure :default @22:15:32.79 SamplePump.configure :default @22:15:32.86 Toroid.configure :default @22:15:32.94 Carousel.configure :default @22:15:33.01 Pressure.configure :default @22:15:33.08 ExtraPump.configure :default @22:15:33.13 PinPort.configure :default @22:15:33.18 All elf controllers running firmware version 4.83 @22:15:33.20 Gate.power :motor,:ON,limit to 5000mA @22:15:33.54 Archive.home.jog 0 @22:15:33.64 Air.home.jog 0 @22:15:33.69 Lysis1.home.jog 0 @22:15:33.77 Lysis2.home.jog 0 @22:15:34.17 Carousel.home.jog 0 @22:15:34.21 Toroid.home.jog 0 @22:15:34.25 PinPort.home.jog 0 @22:15:34.33 Plunge.home.jog 0 @22:15:34.71 Air.in :homing @22:15:34.76 Archive.in :homing @22:15:34.85 Lysis1.in :homing @22:15:34.94 Lysis2.in :homing @22:15:35.02 Air.jog 1382 @22:15:35.08 Lysis1.jog 1393 @22:15:35.12 Archive.jog 1381 @22:15:35.17 Lysis2.jog 1389 @22:15:35.38 PinPort.in :homing @22:15:35.41 Toroid.in :homing @22:15:35.44 Plunge.in :homing @22:15:35.54 PinPort.jog -107 @22:15:35.57 Toroid.jog 619 @22:15:35.61 Plunge.jog 1541 @22:15:35.82 PinPort.setRawPosition! 0 @22:15:35.86 PinPort.seek :home @22:15:35.93 PinPort.in :default @22:15:36.32 Air.setRawPosition! 50 @22:15:36.36 Air.seek :home @22:15:36.45 Lysis1.setRawPosition! 50 @22:15:36.47 Lysis2.setRawPosition! 50 @22:15:36.50 Archive.setRawPosition! 50 @22:15:36.56 Lysis1.seek :home @22:15:36.64 Lysis2.seek :home @22:15:36.67 Archive.seek :home @22:15:37.22 Lysis1.in :default @22:15:37.27 Air.in :default @22:15:37.29 Plunge.setRawPosition! 50 @22:15:37.41 Plunge.seek :home @22:15:37.51 Lysis2.in :default @22:15:37.53 Archive.in :default @22:15:37.69 Plunge.in :default @22:15:37.77 Toroid.setRawPosition! 0 @22:15:37.81 Toroid.seek :home @22:15:37.87 Toroid.in :default @22:15:38.01
Toroid.seek :clear @22:15:39.60 Carousel.in :default @22:15:39.71 Carousel.home.seek 59 @22:15:39.94 Selecting Cartridge 58 @22:15:40.16 Carousel.seek 58 @22:15:40.62 Lysis1.in :fast @22:15:40.67 Lysis1.seek :locked @22:15:43.25 Plunge.seek :locked @22:15:43.37 Air.in :fast @22:15:43.42 Air.seek :locked @22:15:44.98
Carousel.reconfigure :locked @22:15:45.10 Powering cartridge bus @22:15:47.71 Gate.setIR 1750,5000,0,6 @22:15:47.83 Gate.setIR 0,5000,0,6 @22:15:47.87 Gate.power :cartridge,:OFF @22:15:48.40 Mounted archive Cartridge @22:15:48.66 Preparing to filter cartridge 58 @22:15:48.89 Priming sample loop w/100ml, bypass w/10ml @22:15:48.90 SamplePump.setPosition! 0ml @22:15:48.93 Bypass.open @22:15:50.37 Intake.open @22:15:50.57 Sea 2.4m deep (18.2psia) @22:15:50.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @22:15:53.15 Vacuum tare is 60% of full scale @22:15:53.26 Pressure.in :default @22:15:53.67 Pressure.hold @22:15:53.87 SamplePump.in :prime @22:15:53.92 SamplePump.seek 10ml,59 seconds @22:16:02.67 Pressure.stop @22:16:02.72 SamplePump.setPosition! 0ml @22:16:02.76 Exhaust.open @22:16:03.81 Bypass.close @22:16:05.30 Pressure.in :default @22:16:05.35 Pressure.hold @22:16:05.45 Sea 2.2m deep (18.0psia) @22:16:05.57 SamplePump.in :prime @22:16:05.63 SamplePump.seek 100ml,5:16 @22:17:35.61 Pressure.stop @22:17:35.67 Bypass.open @22:17:36.34 Intake.close @22:17:37.07 Toroid.in :hold @22:17:37.12 Toroid.seek :crack @22:17:38.64 Toroid.seek :cartridge @22:17:39.73 Toroid.in :default @22:17:39.78 Toroid.seek :clear @22:17:41.72 Pressurizing cartridge to 26psi above 18.5psia @22:17:41.73 SamplePump.setPosition! 0ml @22:17:41.77 Intake.open @22:17:41.89 Bypass.close @22:17:42.55 Exhaust.close @22:17:43.36 Pressure.in :default @22:17:43.41 Pressure.hold @22:17:43.58 SamplePump.in :charge @22:17:43.63 SamplePump.seek 2.3ml,42 seconds @22:17:50.10 Pressure.stop @22:17:50.14 Intake.close @22:17:50.35 Pressurized cartridge with 0.197ml to 26.5psi @22:17:50.36 Isolated @43.9psia (25.4psi) @22:17:50.37 Verifying cartridge holds at least 0.65..0.85 of 43.9psia @22:17:50.39 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @22:17:55.28 Cartridge pressure fell 2% to 42.9psia @22:17:55.43 Sampling 150.0ml in 1:25:20 @22:17:55.44 SamplePump.setPosition! 0ml @22:17:55.48 Exhaust.open @22:17:56.17 Intake.open @22:17:56.42 Pressure.in :default @22:17:56.46 Pressure.hold @22:17:56.57 Sea 2.9m deep (19.0psia) @22:17:56.58 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @22:17:56.70 SamplePump.in :sample @22:17:56.76 SamplePump.seek 150ml,13:01 @22:17:59.46 Cartridge 58 filtering 1/150ml at 0.50ml/s with 27.6psi vacuum @22:18:59.48 Cartridge 58 filtering 30/150ml at 0.46ml/s with 24.7psi vacuum 1.6m deep (17.1psia) @22:19:59.47 Cartridge 58 filtering 58/150ml at 0.44ml/s with 27.0psi vacuum 1.8m deep (17.4psia) @22:20:59.47 Cartridge 58 filtering 83/150ml at 0.38ml/s with 23.8psi vacuum 1.8m deep (17.3psia) @22:21:59.47 Cartridge 58 filtering 106/150ml at 0.36ml/s with 20.2psi vacuum 3.7m deep (20.2psia) @22:22:59.47 Cartridge 58 filtering 126/150ml at 0.30ml/s with 23.7psi vacuum 1.7m deep (17.1psia) @22:23:59.47 Cartridge 58 filtering 144/150ml at 0.27ml/s with 24.2psi vacuum @22:24:22.18 Pressure.stop @22:24:22.28 Intake.close @22:24:22.33 Exhaust.close @22:24:22.52 Toroid.in :hold @22:24:22.57 Toroid.seek :bypass @22:24:24.19 Toroid.in :default @22:24:24.24 Toroid.seek :clear @22:24:25.84 Sampled 150.0ml @22:24:26.31 Plunge.seek :reagent @22:24:27.10 Sample loop closed. Ready to Deliver Reagents @22:24:27.12 Archive.seek :sealed @22:24:31.03 Archive.seek :evac @22:24:45.06 delay 1:00 @22:25:45.09 Archive.seek :clear @22:26:02.57 RNAlater soak for 10:00 @22:36:02.60 Archive.seek :tVent @22:36:05.03 delay 1 second @22:36:06.05 Archive.seek :evac @22:36:21.56 delay 10 seconds @22:36:31.59 Sample preserved and evacuated @22:36:31.78
Carousel.in :default @22:36:32.25 Carousel.home.seek 58 @22:36:32.55 Plunge.seek :locked @22:36:33.40 Archive.in :unlocking @22:36:33.53 Archive.seek :clear @22:36:33.59 Air.in :unlocking @22:36:33.72 Air.seek :clear @22:36:33.80 Lysis1.in :unlocking @22:36:33.90 Lysis1.seek :clear @22:36:38.08
Gate.power :motor,:OFF @22:36:38.09 Gate.power :core,:OFF @22:36:38.10 Gate.power :analytic1,:OFF @22:36:38.11 Gate.power :analytic2,:OFF @22:36:38.12 Gate.power :analytic3,:OFF @22:36:38.13 Gate.power :main,:OFF @22:36:38.14 Safely stopped and ready to power off @22:36:38.31 Cartridge::None in MAIN -- Not enough dry cartridges remain @09:22:26.46PDT05-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @09:22:26.48 >BREAK< ESP3 Gateway v1.42 for cartridge bus @09:22:28.92 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @09:22:29.37 Sea pressure (Abs) tare is 6% of full scale @09:22:32.89 Polling Can every 2:00 @09:22:32.92 Loading /home/esp/esp2/bin/processAll mission @09:22:33.09 Sea pressure (Abs) tare is 6% of full scale @09:22:33.10 Sampling default goal volumes @09:22:33.11 Cartridge group: dry @09:22:33.24 Gate.power :analytic1,:ON,limit to 5000mA @09:22:33.25 Gate.power :analytic2,:ON,limit to 5000mA @09:22:33.26 Gate.power :analytic3,:ON,limit to 5000mA @09:22:33.85 Archive.configure :default @09:22:33.90 Archive.hold @09:22:33.95 Archive.configure :default @09:22:34.07 Air.configure :default @09:22:34.12 Air.hold @09:22:34.18 Air.configure :default @09:22:34.31 SPR Module 1, FW Rev. B, FPGA Rev. C @09:22:34.32
Lysis1.configure :default @09:22:34.37 Lysis1.hold @09:22:34.42 Lysis1.configure :default @09:22:34.53 Lysis2.configure :default @09:22:34.58 Lysis2.hold @09:22:34.64 Lysis2.configure :default @09:22:34.76 Plunge.configure :default @09:22:34.81 Plunge.hold @09:22:34.87 Plunge.configure :default @09:22:34.99 SamplePump.configure :default @09:22:35.12 Toroid.configure :default @09:22:35.17 Toroid.hold @09:22:35.22 Toroid.configure :default @09:22:35.71 Carousel.configure :default @09:22:35.75 Carousel.hold @09:22:35.81 Carousel.configure :default @09:22:35.93 Pressure.configure :default @09:22:36.05 ExtraPump.configure :default @09:22:36.14 PinPort.configure :default @09:22:36.19 PinPort.hold @09:22:36.25 PinPort.configure :default @09:22:36.30 All elf controllers running firmware version 4.83 @09:22:36.91 Toroid.seek :clear @09:22:36.97 Carousel.in :default @09:22:37.07 Carousel.home.seek 58 @09:22:37.30 Selecting Cartridge 57 @09:22:37.53 Carousel.seek 57 @09:22:38.01 Lysis1.in :fast @09:22:38.06 Lysis1.seek :locked @09:22:40.64 Plunge.seek :locked @09:22:40.76 Air.in :fast @09:22:40.82 Air.seek :locked @09:22:42.38
Carousel.reconfigure :locked @09:22:42.50 Powering cartridge bus @09:22:45.11 Gate.setIR 1750,5000,0,6 @09:22:45.23 Gate.setIR 0,5000,0,6 @09:22:45.28 PuckHeater.configure :default @09:22:45.32 Gate.power :heat,:ON,limit to 5000mA @09:22:46.00 Mounted lysis Cartridge board#Q05092 @09:22:46.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:22:46.27 Preparing to filter cartridge 57 @09:22:46.50 Priming sample loop w/100ml, bypass w/10ml @09:22:46.52 SamplePump.setPosition! 0ml @09:22:46.54 Exhaust.close @09:22:46.60 Bypass.open @09:22:48.03 Intake.open @09:22:48.72 Sea 1.7m deep (17.2psia) @09:22:48.74 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @09:22:51.31 Vacuum tare is 60% of full scale @09:22:51.43 Pressure.in :default @09:22:51.48 Pressure.hold @09:22:51.65 SamplePump.in :prime @09:22:51.71 SamplePump.seek 10ml,59 seconds @09:23:00.43 Pressure.stop @09:23:00.47 SamplePump.setPosition! 0ml @09:23:00.51 Exhaust.open @09:23:01.96 Bypass.close @09:23:03.43 Pressure.in :default @09:23:03.48 Pressure.hold @09:23:03.59 Sea 1.6m deep (17.0psia) @09:23:03.72 SamplePump.in :prime @09:23:03.77 SamplePump.seek 100ml,5:16 @09:24:32.29 Pressure.stop @09:24:32.35 Bypass.open @09:24:33.01 Intake.close @09:24:33.76 Toroid.in :hold @09:24:33.81 Toroid.seek :crack @09:24:35.33 Toroid.seek :cartridge @09:24:36.42 Toroid.in :default @09:24:36.47 Toroid.seek :clear @09:24:38.41 Pressurizing cartridge to 26psi above 17.2psia @09:24:38.42 SamplePump.setPosition! 0ml @09:24:38.46 Intake.open @09:24:38.54 Bypass.close @09:24:39.21 Exhaust.close @09:24:40.04 Pressure.in :default @09:24:40.08 Pressure.hold @09:24:40.26 SamplePump.in :charge @09:24:40.31 SamplePump.seek 2.3ml,42 seconds @09:24:54.04 Pressure.stop @09:24:54.08 Intake.close @09:24:54.22 Pressurized cartridge with 0.900ml to 26.1psi @09:24:54.23 Isolated @41.4psia (24.1psi) @09:24:54.24 Verifying cartridge holds at least 0.65..0.85 of 41.4psia @09:24:54.26 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:24:58.96 Sampling 1000.0ml in 1:25:20 @09:24:58.97 SamplePump.setPosition! 0ml @09:24:59.01 Exhaust.open @09:24:59.74 Intake.open @09:25:00.54 Pressure.in :default @09:25:00.59 Pressure.hold @09:25:00.71 Sea 2.0m deep (17.7psia) @09:25:00.72 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @09:25:00.84 SamplePump.in :sample @09:25:00.90 SamplePump.seek 1000ml,1:23:51 @09:25:03.59 Cartridge 57 filtering 1/1000ml at 0.46ml/s with 24.5psi vacuum @09:26:03.59 Cartridge 57 filtering 30/1000ml at 0.49ml/s with 21.9psi vacuum 2.7m deep (18.7psia) @09:27:03.59 Cartridge 57 filtering 59/1000ml at 0.50ml/s with 25.2psi vacuum @09:28:03.59 Cartridge 57 filtering 88/1000ml at 0.50ml/s with 25.5psi vacuum @09:29:03.59 Cartridge 57 filtering 117/1000ml at 0.50ml/s with 25.7psi vacuum @09:30:03.59 Cartridge 57 filtering 146/1000ml at 0.47ml/s with 21.3psi vacuum @09:31:03.59 Cartridge 57 filtering 175/1000ml at 0.47ml/s with 24.5psi vacuum @09:32:03.59 Cartridge 57 filtering 204/1000ml at 0.49ml/s with 26.0psi vacuum @09:33:03.59 Cartridge 57 filtering 233/1000ml at 0.46ml/s with 23.3psi vacuum 1.8m deep (17.4psia) @09:34:03.59 Cartridge 57 filtering 262/1000ml at 0.50ml/s with 22.7psi vacuum 2.0m deep (17.6psia) @09:35:03.60 Cartridge 57 filtering 291/1000ml at 0.49ml/s with 25.6psi vacuum @09:36:03.59 Cartridge 57 filtering 320/1000ml at 0.47ml/s with 25.0psi vacuum @09:37:03.59 Cartridge 57 filtering 349/1000ml at 0.47ml/s with 24.3psi vacuum @09:38:03.59 Cartridge 57 filtering 378/1000ml at 0.49ml/s with 20.7psi vacuum 2.4m deep (18.2psia) @09:39:03.59 Cartridge 57 filtering 407/1000ml at 0.49ml/s with 22.1psi vacuum 3.3m deep (19.6psia) @10:01:14.37PDT05-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @10:01:14.39 >BREAK< ESP3 Gateway v1.42 for cartridge bus @10:01:16.86 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @10:01:17.34 Sea pressure (Abs) tare is 6% of full scale @10:01:20.96 Polling Can every 2:00 @10:01:20.99 Loading /home/esp/esp2/bin/processAll mission @10:01:21.17 Sea pressure (Abs) tare is 6% of full scale @10:01:21.18 Sampling maximum of 500.0ml per cartridge @10:01:21.19 Cartridge group: dry @10:01:21.32 Gate.power :analytic1,:ON,limit to 5000mA @10:01:21.33 Gate.power :analytic2,:ON,limit to 5000mA @10:01:21.34 Gate.power :analytic3,:ON,limit to 5000mA @10:01:21.93 Archive.configure :default @10:01:21.98 Archive.hold @10:01:22.04 Archive.configure :default @10:01:22.17 Air.configure :default @10:01:22.22 Air.hold @10:01:22.28 SPR Module 1, FW Rev. B, FPGA Rev. C @10:01:22.30
Air.configure :default @10:01:22.41 Lysis1.configure :default @10:01:22.45 Lysis1.hold @10:01:22.51 Lysis1.configure :default @10:01:22.61 Lysis2.configure :default @10:01:22.66 Lysis2.hold @10:01:22.71 Lysis2.configure :default @10:01:22.84 Plunge.configure :default @10:01:22.88 Plunge.hold @10:01:22.94 Plunge.configure :default @10:01:23.07 SamplePump.configure :default @10:01:23.20 Toroid.configure :default @10:01:23.24 Toroid.hold @10:01:23.64 Toroid.configure :default @10:01:23.76 Carousel.configure :default @10:01:23.81 Carousel.hold @10:01:23.86 Carousel.configure :default @10:01:23.98 Pressure.configure :default @10:01:24.11 ExtraPump.configure :default @10:01:24.21 PinPort.configure :default @10:01:24.26 PinPort.hold @10:01:24.31 PinPort.configure :default @10:01:24.36 All elf controllers running firmware version 4.83 @10:01:24.96 Toroid.seek :clear @10:01:25.02 Carousel.in :default @10:01:25.13 Carousel.home.seek 57 @10:01:25.45 Carousel.reconfigure :locked @10:01:25.62 Gate.setIR 1750,5000,0,6 @10:01:25.74 Gate.setIR 0,5000,0,6 @10:01:25.79 PuckHeater.configure :default @10:01:25.83 Gate.power :heat,:ON,limit to 5000mA @10:01:26.52 Mounted lysis Cartridge board#Q05092 @10:01:26.55 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:01:26.58 Selecting Cartridge 57 @10:01:27.08 Preparing to filter cartridge 57 @10:01:27.33 Priming sample loop w/100ml, bypass w/10ml @10:01:27.34 SamplePump.setPosition! 0ml @10:01:27.37 Exhaust.close @10:01:27.43 Bypass.open @10:01:28.89 Intake.open @10:01:29.59 Sea 1.7m deep (17.2psia) @10:01:29.61 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:01:32.17 Vacuum tare is 60% of full scale @10:01:32.29 Pressure.in :default @10:01:32.34 Pressure.hold @10:01:32.89 SamplePump.in :prime @10:01:32.94 SamplePump.seek 10ml,59 seconds @10:01:41.67 Pressure.stop @10:01:41.71 SamplePump.setPosition! 0ml @10:01:41.74 Exhaust.open @10:01:43.13 Bypass.close @10:01:44.65 Pressure.in :default @10:01:44.70 Pressure.hold @10:01:44.81 Sea 1.6m deep (17.1psia) @10:01:44.94 SamplePump.in :prime @10:01:44.99 SamplePump.seek 100ml,5:16 @10:03:15.36 Pressure.stop @10:03:15.41 Bypass.open @10:03:16.09 Intake.close @10:03:16.29 Toroid.in :hold @10:03:16.34 Toroid.seek :crack @10:03:17.86 Toroid.seek :cartridge @10:03:18.96 Toroid.in :default @10:03:19.01 Toroid.seek :clear @10:03:20.95 Pressurizing cartridge to 26psi above 17.2psia @10:03:20.97 SamplePump.setPosition! 0ml @10:03:21.00 Intake.open @10:03:21.24 Bypass.close @10:03:21.90 Exhaust.close @10:03:22.66 Pressure.in :default @10:03:22.71 Pressure.hold @10:03:22.88 SamplePump.in :charge @10:03:22.93 SamplePump.seek 2.3ml,42 seconds @10:03:27.95 Pressure.stop @10:03:27.99 Intake.close @10:03:28.17 Pressurized cartridge with 0.117ml to 26.1psi @10:03:28.18 Isolated @41.4psia (24.2psi) @10:03:28.19 Verifying cartridge holds at least 0.65..0.85 of 41.4psia @10:03:28.21 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:03:32.78 Cartridge pressure fell 7% to 38.3psia @10:03:32.85 Sampling 500.0ml in 1:25:20 @10:03:32.87 SamplePump.setPosition! 0ml @10:03:32.90 Exhaust.open @10:03:33.57 Intake.open @10:03:34.35 Pressure.in :default @10:03:34.40 Pressure.hold @10:03:34.89 Sea 1.7m deep (17.3psia) @10:03:34.90 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @10:03:35.03 SamplePump.in :sample @10:03:35.09 SamplePump.seek 500ml,42:11 @10:03:37.40 Cartridge 57 filtering 1/500ml at 0.47ml/s with 23.6psi vacuum @10:04:37.41 Cartridge 57 filtering 29/500ml at 0.48ml/s with 24.5psi vacuum 2.0m deep (17.7psia) @10:05:37.40 Cartridge 57 filtering 58/500ml at 0.46ml/s with 23.1psi vacuum @10:06:37.41 Cartridge 57 filtering 87/500ml at 0.49ml/s with 23.9psi vacuum 2.7m deep (18.7psia) @10:07:37.41 Cartridge 57 filtering 115/500ml at 0.48ml/s with 22.5psi vacuum 2.6m deep (18.6psia) @10:08:37.41 Cartridge 57 filtering 144/500ml at 0.47ml/s with 24.7psi vacuum 2.2m deep (18.0psia) @10:09:37.41 Cartridge 57 filtering 172/500ml at 0.46ml/s with 25.6psi vacuum @10:10:37.41 Cartridge 57 filtering 201/500ml at 0.47ml/s with 25.6psi vacuum @10:11:37.41 Cartridge 57 filtering 229/500ml at 0.48ml/s with 26.2psi vacuum @10:12:37.41 Cartridge 57 filtering 257/500ml at 0.46ml/s with 22.5psi vacuum 1.9m deep (17.5psia) @10:13:37.41 Cartridge 57 filtering 286/500ml at 0.47ml/s with 25.1psi vacuum @10:14:37.41 Cartridge 57 filtering 314/500ml at 0.48ml/s with 22.8psi vacuum 2.9m deep (19.0psia) @10:15:37.41 Cartridge 57 filtering 343/500ml at 0.47ml/s with 23.4psi vacuum @10:16:37.41 Cartridge 57 filtering 371/500ml at 0.46ml/s with 26.8psi vacuum @10:17:37.41 Cartridge 57 filtering 399/500ml at 0.47ml/s with 26.4psi vacuum @10:18:37.41 Cartridge 57 filtering 428/500ml at 0.47ml/s with 24.7psi vacuum @10:19:37.41 Cartridge 57 filtering 456/500ml at 0.47ml/s with 25.2psi vacuum @10:20:37.41 Cartridge 57 filtering 485/500ml at 0.49ml/s with 23.8psi vacuum 2.5m deep (18.4psia) @10:21:09.37 Pressure.stop @10:21:09.47 Intake.close @10:21:09.53 Exhaust.close @10:21:09.72 Toroid.in :hold @10:21:09.77 Toroid.seek :bypass @10:21:11.39 Toroid.in :default @10:21:11.44 Toroid.seek :clear @10:21:13.04 Sampled 500.0ml @10:21:14.51 SPR.command "read_sequences" @10:21:14.89 SPR.command "stats_on" @10:21:15.00 SPR.command "start 0 1" @10:21:15.73 SPR.start /var/log/esp/spr23apr0510h.spr.bz2 @10:21:17.74 Handoff.select :waste @10:21:24.40 Syringe tare is 16% of full scale @10:21:24.42 PinPort.in :locking @10:21:24.47 PinPort.seek :locked @10:21:24.94 Archive.in :fast @10:21:24.99 Lysis2.in :fast @10:21:25.07 Lysis1.in :fast @10:21:25.12 Archive.seek :full @10:21:25.15 Lysis2.seek :full @10:21:25.26 Lysis1.seek 1350uL @10:21:27.15 Plunge.seek :reagent @10:21:28.01 Evacuating puck with filter bubble point of 50psi @10:21:28.03 PinPort.seek :locked @10:21:28.08 Handoff.select :waste @10:21:34.67 Air syringe pumping up to 1 strokes to reach 8psi @10:21:34.68 Air stroke #1 of 1 @10:21:34.75 Air.in :pressurize @10:21:34.80 Air.seek :pressurized @10:21:40.51 3.1psi after pressurize @10:21:40.52 Air.in :compress @10:21:40.57 Air.seek :evac @10:21:43.56 1.4psi after compress @10:21:54.14 -0.1psi after compress (stable) @10:21:54.17 Air.in :default @10:21:54.23 Air.hold @10:21:54.39 at -0.1psi Opened to waste @10:21:54.40 Handoff.select :blocked @10:21:54.51 Archive syringe pumping up to 8 strokes to reach 50psi @10:21:54.52 Archive stroke #1 of 8 @10:21:54.59 Archive.in :pressurize @10:21:54.64 Archive.seek :pressurized @10:22:07.11 0.1psi after pressurize @10:22:12.68 0.2psi after pressurize (stable) @10:22:12.70 Archive.in :compress @10:22:12.74 Archive.seek :empty @10:22:27.34 26.2psi after compress @10:22:37.92 26.2psi after compress (stable) @10:22:37.94 Archive.in :gulp @10:22:37.98 Archive.seek :topVent @10:22:44.21 26.4psi after gulp @10:22:49.78 26.4psi after gulp (stable) @10:22:49.79 Archive stroke #2 of 8 @10:22:49.85 Archive.in :pressurize @10:22:49.90 Archive.seek :pressurized @10:23:02.40 26.0psi after pressurize @10:23:07.98 26.0psi after pressurize (stable) @10:23:08.00 Archive.in :compress @10:23:08.05 Archive.seek :empty @10:23:22.65 29.9psi after compress @10:23:59.73 34.6psi after compress (stable) @10:23:59.75 Archive.in :gulp @10:23:59.80 Archive.seek :topVent @10:24:06.02 35.7psi after gulp @10:24:11.59 36.1psi after gulp (stable) @10:24:11.60 Archive stroke #3 of 8 @10:24:11.67 Archive.in :pressurize @10:24:11.72 Archive.seek :pressurized @10:24:24.19 36.1psi after pressurize @10:24:29.76 36.3psi after pressurize (stable) @10:24:29.78 Archive.in :compress @10:24:29.83 Archive.seek :empty @10:24:44.46 37.2psi after compress @10:24:55.04 37.4psi after compress (stable) @10:24:55.05 Archive.in :gulp @10:24:55.10 Archive.seek :topVent @10:25:01.33 38.1psi after gulp @10:25:06.90 38.1psi after gulp (stable) @10:25:06.91 Archive stroke #4 of 8 @10:25:06.99 Archive.in :pressurize @10:25:07.04 Archive.seek :pressurized @10:25:19.53 37.7psi after pressurize @10:25:25.12 37.6psi after pressurize (stable) @10:25:25.15 Archive.in :compress @10:25:25.21 Archive.seek :empty @10:25:39.80 38.8psi after compress @10:25:50.38 39.8psi after compress (stable) @10:25:50.39 Archive.in :gulp @10:25:50.44 Archive.seek :topVent @10:25:56.67 40.7psi after gulp @10:26:02.24 40.8psi after gulp (stable) @10:26:02.26 Archive stroke #5 of 8 @10:26:02.32 Archive.in :pressurize @10:26:02.37 Archive.seek :pressurized @10:26:14.84 40.7psi after pressurize @10:26:20.41 40.6psi after pressurize (stable) @10:26:20.43 Archive.in :compress @10:26:20.48 Archive.seek :empty @10:26:35.11 44.4psi after compress @10:26:36.65 Reached 50.3 of 50.0psi after pumping 8650uL (5.00 strokes) @10:26:36.67 Archive.in :default @10:26:36.71 Archive.hold @10:26:36.77 Handoff.select :waste @10:26:47.95 delay 1:00 @10:27:47.96 Handoff.select :blocked @10:28:10.65 Handoff.select :waste @10:28:10.75 2.2psi at evacEnd @10:28:10.76 Handoff.select :blocked @10:28:10.87 Archive.in :fast @10:28:10.92 Archive.seek :topVent @10:28:24.58 Stable @0.8psi after Handoff blocked @10:28:24.59 Archive syringe pumping up to 8 strokes to reach 31psi @10:28:24.60 Archive stroke #1 of 8 @10:28:24.67 Archive.in :pressurize @10:28:24.72 Archive.seek :pressurized @10:28:37.22 1.4psi after pressurize @10:28:37.24 Archive.in :compress @10:28:37.29 Archive.seek :empty @10:28:51.88 2.9psi after compress @10:29:16.96 6.8psi after compress (stable) @10:29:16.98 Archive.in :gulp @10:29:17.03 Archive.seek :topVent @10:29:23.25 7.9psi after gulp @10:29:28.83 8.5psi after gulp (stable) @10:29:28.84 Archive stroke #2 of 8 @10:29:28.91 Archive.in :pressurize @10:29:28.96 Archive.seek :pressurized @10:29:41.47 9.9psi after pressurize @10:29:41.48 Archive.in :compress @10:29:41.53 Archive.seek :empty @10:29:56.13 13.5psi after compress @10:30:47.20 25.8psi after compress (stable) @10:30:47.22 Archive.in :gulp @10:30:47.27 Archive.seek :topVent @10:30:53.49 26.9psi after gulp @10:30:59.07 27.4psi after gulp (stable) @10:30:59.08 Archive stroke #3 of 8 @10:30:59.14 Archive.in :pressurize @10:30:59.20 Archive.seek :pressurized @10:31:11.66 28.7psi after pressurize @10:31:11.68 Archive.in :compress @10:31:11.73 Archive.seek :empty @10:31:25.78 Reached 30.8 of 30.8psi after pumping 5178uL (2.99 strokes) @10:31:25.79 Archive.in :default @10:31:25.84 Archive.hold @10:31:25.90 Handoff.select :waste @10:31:33.07 delay 1:00 @10:32:33.09 Handoff.select :blocked @10:32:55.76 Handoff.select :waste @10:32:55.86 2.7psi at evacEnd @10:32:55.88 Air.seek :fullVent @10:32:57.28 Archive.in :fast @10:32:57.33 Archive.seek :topVent @10:33:04.37 Seawater evacuated, Pressure above/below filter released. @10:33:04.44 Lysis1.in :slow @10:33:04.50 Lysis1.seek 1050uL @10:33:34.50 delay 5 seconds @10:33:39.52 Handoff.select :blocked @10:33:39.69 Lysis1.seek 450uL @10:34:39.66 delay 3 seconds @10:34:42.74 Air.in mix with 1.40048 parts Lysis1 @10:34:43.21 Air.seek :evac @10:34:43.25 Lysis1.seek 300uL @10:34:58.36 Lysis1.seek :empty @10:35:28.43 Lysis buffer delivered @30.5psi @10:35:28.44 Polling Can every 2:00 @10:35:28.98 PuckHeater.configure :default #ambient 20.7C @10:35:29.02 PuckHeater.seek 79.8C @10:36:24.88 PuckHeater.hold 2:00,79.8C @10:38:24.80 Polling Can every 2:00 @10:38:25.34 PuckHeater.configure :default #ambient 20.8C @10:38:25.39 PuckHeater.hold 30 seconds,78.7C @10:38:55.56 Polling Can every 2:00 @10:38:56.10 PuckHeater.configure :default #ambient 20.8C @10:38:56.14 PuckHeater.hold 30 seconds,78.2C @10:39:26.30 Polling Can every 2:00 @10:39:26.84 PuckHeater.configure :default #ambient 20.8C @10:39:26.91 PuckHeater.hold 10:00,77.7C @10:49:26.31 Lysis complete. Syringe pressure @18.2psi @10:49:26.33 Archive syringe pumping up to 5 strokes to reach 40psi Archive stroke #1 of 5 @10:49:26.39 Archive.in :pressurize @10:49:26.45 Archive.seek :pressurized @10:49:38.92 17.6psi after pressurize @10:49:44.51 17.5psi after pressurize (stable) @10:49:44.53 Archive.in :compress @10:49:44.60 Archive.seek :empty @10:49:51.23 Reached 40.9 of 40.0psi after pumping 1557uL (0.90 strokes) @10:49:51.55 Archive.in :default @10:49:51.66 Archive.hold @10:49:51.72 delay 2 seconds @10:49:53.74 Archive.in :fast @10:49:53.79 Archive.seek :full @10:50:00.35 Lysis1.seek 138uL @10:50:14.22 delay 1 second @10:50:15.24 Archive.seek :full @10:50:15.28 Lysis1.seek :empty @10:50:29.19 Handoff.select :blocked @10:50:29.27 Initial air pressure @44.0psi @10:50:29.34 Lysis1.in :extract @10:50:29.40 Lysis1.seek 600uL,nil @10:50:31.74 Archive syringe pumping up to 6 strokes to reach 25psi @10:50:31.75 Archive stroke #1 of 6 @10:50:31.88 Archive.in :pressurize @10:50:31.93 Archive.seek :pressurized @10:50:44.41 11.1psi after pressurize @10:50:49.99 11.0psi after pressurize (stable) @10:50:50.01 Archive.in :compress @10:50:50.06 Archive.seek :empty @10:50:55.29 Archive.in :default @10:50:55.34 Archive.hold @10:50:55.51 Initial 600uL lysate @16.5psi @10:50:55.52 Handoff.select :blocked @10:50:55.60 Initial air pressure @16.7psi @10:50:55.67 Lysis1.seek 825uL,nil @10:50:57.40 Archive syringe pumping up to 6 strokes to reach 17psi @10:50:57.41 Archive stroke #1 of 6 @10:50:57.48 Archive.in :compress @10:50:57.54 Archive.seek :empty @10:50:59.94 Archive.in :default @10:51:00.00 Archive.hold @10:51:00.23 Recovered 825uL lysate @14.8psi @10:51:00.25 Air.in :halfSpeed @10:51:00.30 Air.seek :fullVent @10:51:02.49 Puck Top Vented, bottom @1.3psi @10:51:02.50 Handoff.select :waste @10:51:02.61 delay 5 seconds @10:51:07.67 Handoff open to waste, bottom @0.2psi @10:51:07.68 Air.in :default @10:51:07.76 Air.seek :evac @10:51:09.16 Lysis2.in :DAmix @10:51:09.26 Lysis1.in :DAmix @10:51:09.79 Lysis2.seek 1480uL @10:51:09.83 Lysis1.seek 575uL @10:51:44.92 delay 5 seconds @10:51:49.94 Handoff.select SPR @10:51:50.05 SPR.command "start 2 14" @10:51:50.76 SPR stabilized @10:51:50.88 delay 10 seconds @10:52:00.89 SPR.command "start 1 0" @10:52:04.63 Waiting up to 4:00 for SPR to enter state 1 @10:54:09.35 SPR.command "hold_on" @10:54:10.75 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:54:11.22 Lysis2.seek 1180uL @10:54:11.27 Lysis1.seek 275uL @10:54:53.30 delay 15 seconds @10:55:08.31 SPR.command "hold_off" @10:55:11.40 Waiting up to 38:00 for SPR to enter state 7 @11:13:11.01 SPRsummary:-30.7RIU,96.9RIU,77.3RIU,136RIU,7.44ng/L,9.44ng/L,12.8ng/L after 3291s @11:19:48.22 SPR analysis completed @11:19:54.90 Ended log spr23apr0510h.spr.bz2 @11:19:54.91 SPR.command "stats_off" @11:19:55.43 Handoff.select :close @11:19:55.55
Gate.power :heat,:OFF @11:19:55.57 Gate.power :cartridge,:OFF @11:19:55.63 PinPort.in :unlocking @11:19:55.68 PinPort.seek :clear @11:19:56.14 Carousel.in :default @11:19:56.24 Carousel.home.seek 57 @11:19:56.52 Plunge.seek :locked @11:19:57.37 Archive.in :unlocking @11:19:57.49 Archive.seek :clear @11:19:57.57 Air.in :unlocking @11:19:58.04 Air.seek :clear @11:19:58.11 Lysis1.in :unlocking @11:19:58.24 Lysis1.seek :clear @11:19:58.30 Lysis2.in :unlocking @11:19:58.38 Lysis2.seek :clear @11:20:02.32
Gate.power :motor,:OFF @11:20:02.33 Gate.power :core,:OFF @11:20:02.34 Gate.power :analytic1,:OFF @11:20:02.35 Gate.power :analytic2,:OFF @11:20:02.36 Gate.power :analytic3,:OFF @11:20:02.37 Gate.power :main,:OFF @11:20:02.38 Safely stopped and ready to power off @11:20:02.81 Cartridge::None in MAIN -- Not enough dry cartridges remain @11:23:24.38PDT05-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @11:23:24.40 >BREAK< ESP3 Gateway v1.42 for cartridge bus @11:23:26.80 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @11:23:27.25 Sea pressure (Abs) tare is 6% of full scale @11:23:30.78 Polling Can every 2:00 @11:23:30.81 Loading /home/esp/esp2/bin/processAll mission @11:23:30.98 Sea pressure (Abs) tare is 6% of full scale @11:23:30.99 Sampling default goal volumes @11:23:31.00 Cartridge group: dry @11:23:31.04 Gate.power :main,:ON,limit to 5000mA @11:23:31.06 Gate.power :analytic1,:ON,limit to 5000mA @11:23:31.07 Gate.power :analytic2,:ON,limit to 5000mA @11:23:31.08 Gate.power :analytic3,:ON,limit to 5000mA @11:23:31.18 Powering core bus @11:23:33.48 SPR Module 1, FW Rev. B, FPGA Rev. C @11:23:33.88
Archive.configure :default @11:23:33.95 Air.configure :default @11:23:34.00 Lysis1.configure :default @11:23:34.05 Lysis2.configure :default @11:23:34.12 Plunge.configure :default @11:23:34.20 SamplePump.configure :default @11:23:34.27 Toroid.configure :default @11:23:34.34 Carousel.configure :default @11:23:34.42 Pressure.configure :default @11:23:34.49 ExtraPump.configure :default @11:23:34.54 PinPort.configure :default @11:23:34.59 All elf controllers running firmware version 4.83 @11:23:34.61 Gate.power :motor,:ON,limit to 5000mA @11:23:34.79 Archive.home.jog 0 @11:23:35.30 Carousel.home.jog 0 @11:23:35.33 Toroid.home.jog 0 @11:23:35.37 Lysis2.home.jog 0 @11:23:35.38 Lysis1.home.jog 0 @11:23:35.75 Plunge.home.jog 0 @11:23:35.81 PinPort.home.jog 0 @11:23:35.83 Air.home.jog 0 @11:23:36.29 Archive.in :homing @11:23:36.57 Archive.jog 1380 @11:23:36.61 Toroid.in :homing @11:23:36.64 Plunge.in :homing @11:23:36.67 Lysis2.in :homing @11:23:36.70 Lysis1.in :homing @11:23:36.73 Air.in :homing @11:23:36.77 PinPort.in :homing @11:23:37.00 Toroid.jog 620 @11:23:37.03 Plunge.jog 1544 @11:23:37.05 Lysis2.jog 1388 @11:23:37.06 Air.jog 1379 @11:23:37.09 Lysis1.jog 1392 @11:23:37.11 PinPort.jog -106 @11:23:37.46 PinPort.setRawPosition! 0 @11:23:37.50 PinPort.seek :home @11:23:37.56 PinPort.in :default @11:23:37.94 Archive.setRawPosition! 50 @11:23:37.98 Archive.seek :home @11:23:38.23 Archive.in :default @11:23:38.34 Air.setRawPosition! 50 @11:23:38.39 Air.seek :home @11:23:38.45 Plunge.setRawPosition! 50 @11:23:38.49 Lysis2.setRawPosition! 50 @11:23:38.50 Lysis1.setRawPosition! 50 @11:23:38.58 Plunge.seek :home @11:23:38.62 Lysis2.seek :home @11:23:38.67 Lysis1.seek :home @11:23:38.80 Air.in :default @11:23:38.90 Plunge.in :default @11:23:38.98 Lysis2.in :default @11:23:39.01 Lysis1.in :default @11:23:39.21 Toroid.setRawPosition! 0 @11:23:39.25 Toroid.seek :home @11:23:39.32 Toroid.in :default @11:23:39.40
Toroid.seek :clear @11:23:41.01 Carousel.in :default @11:23:41.11 Carousel.home.seek 57 @11:23:41.34 Selecting Cartridge 56 @11:23:41.57 Carousel.seek 56 @11:23:42.03 Lysis1.in :fast @11:23:42.08 Lysis1.seek :locked @11:23:44.66 Plunge.seek :locked @11:23:44.78 Air.in :fast @11:23:44.83 Air.seek :locked @11:23:46.40
Carousel.reconfigure :locked @11:23:46.52 Powering cartridge bus @11:23:49.13 Gate.setIR 1750,5000,0,6 @11:23:49.55 Gate.setIR 0,5000,0,6 @11:23:49.66 Gate.power :cartridge,:OFF @11:23:50.19 Mounted archive Cartridge @11:23:50.45 Preparing to filter cartridge 56 @11:23:50.67 Priming sample loop w/100ml, bypass w/10ml @11:23:50.68 SamplePump.setPosition! 0ml @11:23:50.71 Bypass.open @11:23:52.16 Intake.open @11:23:52.88 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:23:55.46 Vacuum tare is 60% of full scale @11:23:55.58 Pressure.in :default @11:23:55.62 Pressure.hold @11:23:55.79 SamplePump.in :prime @11:23:55.85 SamplePump.seek 10ml,59 seconds @11:24:04.58 Pressure.stop @11:24:04.62 SamplePump.setPosition! 0ml @11:24:04.66 Exhaust.open @11:24:06.10 Bypass.close @11:24:07.57 Pressure.in :default @11:24:07.62 Pressure.hold @11:24:07.84 SamplePump.in :prime @11:24:07.90 SamplePump.seek 100ml,5:16 @11:25:35.31 Pressure.stop @11:25:35.37 Bypass.open @11:25:36.05 Intake.close @11:25:36.43 Toroid.in :hold @11:25:36.48 Toroid.seek :crack @11:25:38.00 Toroid.seek :cartridge @11:25:39.09 Toroid.in :default @11:25:39.14 Toroid.seek :clear @11:25:41.08 Pressurizing cartridge to 26psi above 17.2psia @11:25:41.09 SamplePump.setPosition! 0ml @11:25:41.13 Intake.open @11:25:41.79 Bypass.close @11:25:42.46 Exhaust.close @11:25:43.28 Pressure.in :default @11:25:43.33 Pressure.hold @11:25:43.88 SamplePump.in :charge @11:25:43.94 SamplePump.seek 2.3ml,42 seconds @11:25:49.15 Pressure.stop @11:25:49.19 Intake.close @11:25:49.33 Pressurized cartridge with 0.127ml to 26.2psi @11:25:49.34 Isolated @43.2psia (26.1psi) @11:25:49.35 Verifying cartridge holds at least 0.65..0.85 of 43.2psia @11:25:49.37 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:25:53.94 Cartridge pressure fell 3% to 41.9psia @11:25:54.02 Sampling 1000.0ml in 1:25:20 @11:25:54.03 SamplePump.setPosition! 0ml @11:25:54.07 Exhaust.open @11:25:54.81 Intake.open @11:25:55.58 Pressure.in :default @11:25:55.63 Pressure.hold @11:25:55.73 Sea 2.0m deep (17.6psia) @11:25:55.74 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @11:25:55.86 SamplePump.in :sample @11:25:55.92 SamplePump.seek 1000ml,1:23:51 @11:25:58.67 Cartridge 56 filtering 1/1000ml at 0.47ml/s with 22.1psi vacuum @11:26:58.63 Cartridge 56 filtering 30/1000ml at 0.48ml/s with 22.4psi vacuum 3.0m deep (19.2psia) @11:27:58.62 Cartridge 56 filtering 58/1000ml at 0.47ml/s with 24.4psi vacuum @11:28:58.63 Cartridge 56 filtering 86/1000ml at 0.47ml/s with 24.9psi vacuum @11:29:58.63 Cartridge 56 filtering 115/1000ml at 0.46ml/s with 23.8psi vacuum @11:30:58.63 Cartridge 56 filtering 143/1000ml at 0.47ml/s with 22.6psi vacuum @11:31:58.63 Cartridge 56 filtering 170/1000ml at 0.47ml/s with 20.8psi vacuum @11:32:58.62 Cartridge 56 filtering 198/1000ml at 0.45ml/s with 22.4psi vacuum @11:33:58.63 Cartridge 56 filtering 226/1000ml at 0.47ml/s with 22.6psi vacuum @11:34:58.63 Cartridge 56 filtering 253/1000ml at 0.47ml/s with 25.3psi vacuum @11:35:58.63 Cartridge 56 filtering 281/1000ml at 0.46ml/s with 24.0psi vacuum @11:36:58.63 Cartridge 56 filtering 308/1000ml at 0.47ml/s with 26.6psi vacuum @11:37:58.63 Cartridge 56 filtering 336/1000ml at 0.47ml/s with 24.3psi vacuum 1.8m deep (17.4psia) @11:38:58.63 Cartridge 56 filtering 363/1000ml at 0.47ml/s with 22.6psi vacuum @11:39:58.63 Cartridge 56 filtering 391/1000ml at 0.45ml/s with 24.3psi vacuum @11:40:58.63 Cartridge 56 filtering 418/1000ml at 0.46ml/s with 23.0psi vacuum @11:41:58.62 Cartridge 56 filtering 446/1000ml at 0.45ml/s with 21.8psi vacuum 1.6m deep (17.0psia) @11:42:58.95 Cartridge 56 filtering 473/1000ml at 0.45ml/s with 26.8psi vacuum @11:43:58.63 Cartridge 56 filtering 501/1000ml at 0.45ml/s with 26.3psi vacuum @11:44:58.63 Cartridge 56 filtering 528/1000ml at 0.46ml/s with 23.7psi vacuum @11:45:58.63 Cartridge 56 filtering 556/1000ml at 0.47ml/s with 22.0psi vacuum @11:46:58.63 Cartridge 56 filtering 583/1000ml at 0.46ml/s with 26.1psi vacuum @11:47:58.63 Cartridge 56 filtering 611/1000ml at 0.45ml/s with 24.1psi vacuum @11:48:58.63 Cartridge 56 filtering 639/1000ml at 0.47ml/s with 22.4psi vacuum 2.8m deep (18.9psia) @11:49:58.63 Cartridge 56 filtering 666/1000ml at 0.45ml/s with 23.9psi vacuum @11:50:58.63 Cartridge 56 filtering 694/1000ml at 0.47ml/s with 22.8psi vacuum 3.0m deep (19.2psia) @11:51:58.63 Cartridge 56 filtering 722/1000ml at 0.46ml/s with 23.9psi vacuum @11:52:58.63 Cartridge 56 filtering 749/1000ml at 0.46ml/s with 23.9psi vacuum 2.1m deep (17.8psia) @11:53:58.63 Cartridge 56 filtering 777/1000ml at 0.46ml/s with 24.3psi vacuum @11:54:58.63 Cartridge 56 filtering 805/1000ml at 0.46ml/s with 25.5psi vacuum @11:55:58.63 Cartridge 56 filtering 833/1000ml at 0.46ml/s with 24.6psi vacuum @11:56:58.63 Cartridge 56 filtering 861/1000ml at 0.48ml/s with 22.9psi vacuum 2.5m deep (18.4psia) @11:57:58.63 Cartridge 56 filtering 889/1000ml at 0.47ml/s with 26.3psi vacuum @11:58:58.62 Cartridge 56 filtering 917/1000ml at 0.46ml/s with 24.3psi vacuum @11:59:58.63 Cartridge 56 filtering 945/1000ml at 0.46ml/s with 24.0psi vacuum @12:00:58.63 Cartridge 56 filtering 973/1000ml at 0.48ml/s with 24.6psi vacuum 1.9m deep (17.5psia) @12:01:56.01 Pressure.stop @12:01:56.11 Intake.close @12:01:56.16 Exhaust.close @12:01:56.34 Toroid.in :hold @12:01:56.40 Toroid.seek :bypass @12:01:58.01 Toroid.in :default @12:01:58.06 Toroid.seek :clear @12:01:59.66 Sampled 1000.0ml @12:02:00.13 Plunge.seek :reagent @12:02:00.92 Sample loop closed. Ready to Deliver Reagents @12:02:00.94 Archive.seek :sealed @12:02:04.85 Archive.seek :evac @12:02:18.88 delay 1:00 @12:03:18.90 Archive.seek :clear @12:03:36.38 RNAlater soak for 10:00 @12:13:36.41 Archive.seek :tVent @12:13:38.84 delay 1 second @12:13:39.86 Archive.seek :evac @12:13:55.36 delay 10 seconds @12:14:05.39 Sample preserved and evacuated @12:14:05.96
Carousel.in :default @12:14:06.06 Carousel.home.seek 56 @12:14:06.34 Plunge.seek :locked @12:14:07.19 Archive.in :unlocking @12:14:07.33 Air.in :unlocking @12:14:07.36 Archive.seek :clear @12:14:07.47 Air.seek :clear @12:14:07.56 Lysis1.in :unlocking @12:14:07.69 Lysis1.seek :clear @12:14:11.96
Gate.power :motor,:OFF @12:14:11.97 Gate.power :core,:OFF @12:14:11.98 Gate.power :analytic1,:OFF @12:14:11.99 Gate.power :analytic2,:OFF @12:14:12.00 Gate.power :analytic3,:OFF @12:14:12.01 Gate.power :main,:OFF @12:14:12.02 Safely stopped and ready to power off @12:14:12.45 Cartridge::None in MAIN -- Not enough dry cartridges remain nohup: procesAll: No such file or directory @18:27:42.85PDT13-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @18:27:42.87 >BREAK< ESP3 Gateway v1.42 for cartridge bus @18:27:45.28 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @18:27:45.73 Sea pressure (Abs) tare is 6% of full scale @18:27:49.67 Polling Can every 2:00 @18:27:49.70 Loading /home/esp/esp2/bin/processAll mission @18:27:50.65 Sea pressure (Abs) tare is 6% of full scale @18:27:50.66 Sampling default goal volumes @18:27:50.70 GoodNews.email "Cartridge group: dry\n\n",Subject: "Begun processAll --mail" @18:27:51.32
Gate.power :analytic1,:ON,limit to 5000mA @18:27:51.34 Gate.power :analytic2,:ON,limit to 5000mA @18:27:51.35 Gate.power :analytic3,:ON,limit to 5000mA @18:27:51.98 Archive.configure :default @18:27:52.04 Archive.hold @18:27:52.11 Archive.configure :default @18:27:52.25 Air.configure :default @18:27:52.34 SPR Module 1, FW Rev. B, FPGA Rev. C @18:27:52.38
Air.hold @18:27:52.47 Air.configure :default @18:27:52.57 Lysis1.configure :default @18:27:52.62 Lysis1.hold @18:27:52.67 Lysis1.configure :default @18:27:52.77 Lysis2.configure :default @18:27:52.82 Lysis2.hold @18:27:52.88 Lysis2.configure :default @18:27:53.02 Plunge.configure :default @18:27:53.06 Plunge.hold @18:27:53.11 Plunge.configure :default @18:27:53.23 SamplePump.configure :default @18:27:53.36 Toroid.configure :default @18:27:53.40 Toroid.hold @18:27:53.45 Toroid.configure :default @18:27:53.57 Carousel.configure :default @18:27:53.62 Carousel.hold @18:27:53.67 Carousel.configure :default @18:27:53.79 Pressure.configure :default @18:27:53.91 ExtraPump.configure :default @18:27:54.00 PinPort.configure :default @18:27:54.05 PinPort.hold @18:27:54.10 PinPort.configure :default @18:27:54.15 All elf controllers running firmware version 4.83 @18:27:54.74 Toroid.seek :clear @18:27:54.79 Carousel.in :default @18:27:54.89 Carousel.home.seek 57 @18:27:55.21 Carousel.reconfigure :locked @18:27:55.38 Gate.setIR 1750,5000,0,6 @18:27:55.49 Gate.setIR 0,5000,0,6 @18:27:55.55 PuckHeater.configure :default @18:27:55.59 Gate.power :heat,:ON,limit to 5000mA @18:27:56.62 Mounted lysis Cartridge board#Q05080 @18:27:56.65 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:27:56.69 Selecting Cartridge 60 @18:27:56.81 Gate.power :heat,:OFF @18:27:56.82 Gate.power :cartridge,:OFF @18:27:56.88 Carousel.in :default @18:27:56.98 Carousel.home.seek 57 @18:27:57.32 Air.in :unlocking @18:27:57.43 Air.seek :clear @18:27:58.76 Lysis1.in :unlocking @18:27:58.89 Lysis1.seek :clear @18:27:58.92 Plunge.in :unlocking @18:27:59.02 Plunge.seek :clear @18:28:00.26
Carousel.seek 60 @18:28:01.13 Lysis1.in :fast @18:28:01.18 Lysis1.seek :locked @18:28:03.76 Plunge.in :default @18:28:03.82 Plunge.seek :locked @18:28:03.91 Air.in :fast @18:28:03.96 Air.seek :locked @18:28:05.52
Carousel.reconfigure :locked @18:28:05.64 Powering cartridge bus @18:28:08.25 Gate.setIR 1750,5000,0,6 @18:28:08.37 Gate.setIR 0,5000,0,6 @18:28:08.42 PuckHeater.configure :default @18:28:08.46 Gate.power :heat,:ON,limit to 5000mA @18:28:09.10 Mounted lysis Cartridge board#Q05062 @18:28:09.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:28:09.21 GoodNews.email "Filtering up to 1000ml",Subject: "Sampling daSPR_phyto cartridge in slot 60" @18:28:09.48 Preparing to filter cartridge 60 @18:28:09.75 Priming sample loop w/100ml, bypass w/10ml @18:28:09.77 SamplePump.setPosition! 0ml @18:28:09.81 Exhaust.close @18:28:09.87 Bypass.open @18:28:11.32 Intake.open @18:28:12.42 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @18:28:15.00 Vacuum tare is 60% of full scale @18:28:15.11 Pressure.in :default @18:28:15.16 Pressure.hold @18:28:15.33 SamplePump.in :prime @18:28:15.39 SamplePump.seek 10ml,59 seconds @18:28:24.11 Pressure.stop @18:28:24.15 SamplePump.setPosition! 0ml @18:28:24.19 Exhaust.open @18:28:25.60 Bypass.close @18:28:27.07 Pressure.in :default @18:28:27.12 Pressure.hold @18:28:27.34 SamplePump.in :prime @18:28:27.40 SamplePump.seek 100ml,5:16 @18:29:52.45 Pressure.stop @18:29:52.51 Bypass.open @18:29:53.17 Intake.close @18:29:53.79 Toroid.in :hold @18:29:53.84 Toroid.seek :crack @18:29:55.36 Toroid.seek :cartridge @18:29:56.45 Toroid.in :default @18:29:56.50 Toroid.seek :clear @18:29:58.45 Pressurizing cartridge to 26psi above 15.1psia @18:29:58.46 SamplePump.setPosition! 0ml @18:29:58.50 Intake.open @18:29:59.14 Bypass.close @18:29:59.80 Exhaust.close @18:30:00.55 Pressure.in :default @18:30:00.60 Pressure.hold @18:30:00.76 SamplePump.in :charge @18:30:00.82 SamplePump.seek 2.3ml,42 seconds @18:30:13.77 Pressure.stop @18:30:13.81 Intake.close @18:30:14.49 Pressurized cartridge with 0.800ml to 26.1psi @18:30:14.50 Isolated @39.5psia (24.4psi) @18:30:14.51 Verifying cartridge holds at least 0.65..0.85 of 39.5psia @18:30:14.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @18:30:19.17 Sampling 1000.0ml in 1:25:20 @18:30:19.18 SamplePump.setPosition! 0ml @18:30:19.22 Exhaust.open @18:30:19.90 Intake.open @18:30:20.69 Pressure.in :default @18:30:20.74 Pressure.hold @18:30:20.84 Sea 2.3m deep (18.1psia) @18:30:20.85 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @18:30:20.97 SamplePump.in :sample @18:30:21.03 SamplePump.seek 1000ml,1:23:51 @18:30:23.73 Cartridge 60 filtering 1/1000ml at 0.45ml/s with 23.4psi vacuum @18:31:23.74 Cartridge 60 filtering 29/1000ml at 0.47ml/s with 23.4psi vacuum @18:32:23.74 Cartridge 60 filtering 56/1000ml at 0.46ml/s with 24.6psi vacuum @18:33:23.74 Cartridge 60 filtering 83/1000ml at 0.43ml/s with 21.9psi vacuum @18:34:23.74 Cartridge 60 filtering 108/1000ml at 0.41ml/s with 22.8psi vacuum @18:35:23.74 Cartridge 60 filtering 131/1000ml at 0.39ml/s with 24.5psi vacuum @18:36:23.74 Cartridge 60 filtering 153/1000ml at 0.36ml/s with 23.3psi vacuum @18:37:23.74 Cartridge 60 filtering 174/1000ml at 0.32ml/s with 24.8psi vacuum @18:38:23.74 Cartridge 60 filtering 193/1000ml at 0.30ml/s with 24.7psi vacuum @18:39:23.74 Cartridge 60 filtering 211/1000ml at 0.31ml/s with 23.6psi vacuum @18:40:23.74 Cartridge 60 filtering 228/1000ml at 0.30ml/s with 20.7psi vacuum @18:41:23.74 Cartridge 60 filtering 245/1000ml at 0.24ml/s with 19.6psi vacuum 1.7m deep (17.2psia) @18:42:23.74 Cartridge 60 filtering 261/1000ml at 0.23ml/s with 24.8psi vacuum @18:43:23.74 Cartridge 60 filtering 276/1000ml at 0.26ml/s with 24.6psi vacuum @18:44:23.74 Cartridge 60 filtering 290/1000ml at 0.20ml/s with 24.5psi vacuum @18:45:23.74 Cartridge 60 filtering 303/1000ml at 0.21ml/s with 24.1psi vacuum @18:46:23.74 Cartridge 60 filtering 316/1000ml at 0.20ml/s with 24.7psi vacuum @18:47:18.27 Flow averaged 0.32ml/s over 16:58 @18:47:18.30 Waiting up to 14 seconds for vacuum to reduce from 28.6 to 18.0psi @18:47:19.12 Relieved excess vacuum in .9 seconds @18:47:19.15 SamplePump.seek 1000ml,56:36 @18:47:23.73 Cartridge 60 filtering 328/1000ml at 0.20ml/s with 28.3psi vacuum @18:47:28.89 Flow averaged 0.21ml/s over 10 seconds @18:47:28.92 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @18:47:29.74 Relieved excess vacuum in .9 seconds @18:47:29.76 SamplePump.seek 1000ml,56:26 @18:47:36.44 Flow estimate reduced to 0.17ml/s @18:47:36.46 Intake.close @18:47:36.51 Exhaust.close @18:47:36.72 Toroid.in :hold @18:47:36.78 Toroid.seek :bypass @18:47:38.42 Toroid.in :default @18:47:38.47 Toroid.seek :clear @18:47:40.07 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @18:47:40.09 Pressure.stop @18:47:40.25 Sampled 330.3ml @18:47:40.62 GoodNews.email "After filtering 330.3ml",Subject: "Processing daSPR_phyto cartridge in slot 60" @18:47:42.13 SPR.command "read_sequences" @18:47:42.17 SPR.command "stats_on" @18:47:42.29 SPR.command "start 0 1" @18:47:43.01 SPR.start /var/log/esp/spr23apr1318h.spr.bz2 @18:47:44.93 Handoff.select :waste @18:47:51.61 Syringe tare is 17% of full scale @18:47:51.63 PinPort.in :locking @18:47:51.68 PinPort.seek :locked @18:47:52.15 Archive.in :fast @18:47:52.18 Lysis2.in :fast @18:47:52.24 Lysis1.seek 1350uL @18:47:52.36 Lysis2.seek :full @18:47:52.41 Archive.seek :full @18:47:54.72 Plunge.seek :reagent @18:47:55.58 Evacuating puck with filter bubble point of 50psi @18:47:55.60 PinPort.seek :locked @18:47:55.65 Handoff.select :waste @18:48:02.73 Air syringe pumping up to 1 strokes to reach 8psi @18:48:02.74 Air stroke #1 of 1 @18:48:02.81 Air.in :pressurize @18:48:02.86 Air.seek :pressurized @18:48:08.56 0.2psi after pressurize @18:48:08.58 Air.in :compress @18:48:08.63 Air.seek :evac @18:48:11.60 0.6psi after compress @18:48:22.18 0.1psi after compress (stable) @18:48:22.19 Air.in :default @18:48:22.56 Air.hold @18:48:22.80 at 0.0psi Opened to waste @18:48:22.81 Handoff.select :blocked @18:48:22.93 Archive syringe pumping up to 8 strokes to reach 50psi @18:48:22.94 Archive stroke #1 of 8 @18:48:23.00 Archive.in :pressurize @18:48:23.05 Archive.seek :pressurized @18:48:35.50 3.5psi after pressurize @18:49:08.58 16.3psi after pressurize (stable) @18:49:08.60 Archive.in :compress @18:49:08.66 Archive.seek :empty @18:49:20.33 Archive.in :default @18:49:20.38 Archive.hold @18:49:20.44 Handoff.select :blocked @18:49:20.53 Archive.setRawPosition! -8007 @18:49:20.58 Slide::Error in PROCESSING -- Archive Syringe (ArS) positionErr at 62uL (sensed 86uL) @18:49:20.60 Waiting to Resume from Checkpoint "Archive Syringe (ArS) positionErr at 62uL (sensed 86uL)" @18:49:20.61 BadNews.email "Archive Syringe (ArS) positionErr at 62uL (sensed 86uL)",Subject: "Slide::Error in PROCESSING" @18:59:24.20 Ended log spr23apr1318h.spr.bz2 @18:59:24.21 SPR.command "stats_off" @19:19:22.52 Session Begun @19:19:28.74 -> exit @19:19:28.75 Session Ended @19:29:25.09 Session Begun @19:32:11.17 -> Handoff Handoff Valve::Manifold selects blocked @19:32:22.75 -> Air Air Syringe (AirS) at evac @19:32:47.99 -> Syringe.psi 19.14004501332 @19:32:56.24 -> Archive Archive Syringe (ArS) at 76uL @19:33:05.46 -> Archive.evac @19:33:05.48 Archive.seek :evac Archive Syringe (ArS) empty @19:33:27.37 -> Archive Archive Syringe (ArS) empty @19:34:01.03 -> Archive.pumpTo 50 @19:34:01.05 Archive syringe pumping up to 8 strokes to reach 50psi @19:34:01.07 Archive stroke #1 of 8 @19:34:01.16 Archive.in :compress @19:34:01.23 Archive.seek :empty @19:34:01.39 19.2psi after compress @19:34:01.50 Archive.in :default @19:34:01.57 Archive.hold @19:34:01.65 Slide::Error in preston -- Archive Syringe (ArS) positionErr at empty (sensed 16uL) while reading Air Syringe (AirS)'s status @19:34:39.46 -> Syringe.psi 19.2088872173843 @19:37:11.90 -> BACsampler.evac2waste @19:37:11.91 Handoff.select :waste @19:37:19.11 delay 1:00 @19:38:19.14 Handoff.select :blocked @19:38:41.82 Handoff.select :waste @19:38:41.94 -0.0psi at evacEnd @19:38:55.39 -> Archive @19:38:55.48 Slide::Error in preston -- Archive Syringe (ArS) Not yet Homed! @19:39:07.54 -> Archive.home.to :empty @19:39:07.55 Archive.in :default @19:39:07.63 Archive.home.seek :empty Archive Syringe (ArS) empty @19:39:14.15 -> Archive Archive Syringe (ArS) empty @19:39:28.35 -> Syringe Archive = Archive Syringe (ArS) empty Air = Air Syringe (AirS) at evac Lysis1 = Lysis Syringe #1 (LyS1) at 1350uL Lysis2 = Lysis Syringe #2 (LyS2) locked @19:39:31.82 -> Syringe.psi -0.0990562825147343 @19:39:35.97 -> Archive.full @19:39:35.99 Archive.seek :full Archive Syringe (ArS) full @19:40:03.21 -> Handoff.blocked @19:40:03.23 Handoff.select :blocked Handoff Valve::Manifold selects blocked @19:40:05.93 -> Syringe.psi -0.0868176684588597 @19:40:14.17 -> Archive.pumpTo 50 @19:40:14.19 Archive syringe pumping up to 8 strokes to reach 50psi @19:40:14.21 Archive stroke #1 of 8 @19:40:14.31 Archive.in :pressurize @19:40:14.39 Archive.seek :pressurized @19:40:26.86 -0.1psi after pressurize @19:40:32.44 -0.1psi after pressurize (stable) @19:40:32.45 Archive.in :compress @19:40:32.54 Archive.seek :empty @19:40:42.96 Archive.in :default @19:40:43.03 Archive.hold @19:40:43.11 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 90uL (sensed 115uL) @19:41:06.40 -> Archive.empty @19:41:06.41 Archive.seek :empty @19:41:06.52 Slide::Error in preston -- Archive Syringe (ArS) notReady at 90uL (sensed emptyAr) @19:41:18.48 -> Archive.home.jog 0 @19:41:18.50 Archive.in :default @19:41:18.58 Archive.home.jog 0 Archive Syringe (ArS) at 103uL @19:41:21.69 -> Archive.empty @19:41:21.71 Archive.seek :empty @19:41:22.33 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 68uL (sensed 98uL) @19:41:30.75 -> Archive.home.jog 0 @19:41:30.77 Archive.in :default @19:41:30.85 Archive.home.jog 0 Archive Syringe (ArS) at 97uL @19:41:32.68 -> Archive.empty @19:41:32.70 Archive.seek :empty @19:41:33.29 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 64uL (sensed 95uL) @19:42:22.80 -> Syringe.psi 6.20535978301763 @19:43:50.90 -> Archive.goHome! @19:43:50.92 Archive.in :default @19:43:51.00 Archive.home.jog 0 @19:43:51.17 Archive.in :homing @19:43:51.23 Archive.jog 9265 @19:43:56.07 Archive.setRawPosition! 50 @19:43:56.13 Archive.seek :home @19:43:56.40 Archive.in :default Archive Syringe (ArS) clear @19:44:01.75 -> Syringe.psi 10.5944327488056 @19:44:15.87 -> Archive.evac; Syringe.psi @19:44:15.89 Archive.seek :evac @19:44:31.84 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 158uL (sensed 188uL) @19:44:56.14 -> Syringe.psi 12.5174249823349 @19:45:22.32 -> Archive.home.jog 0; Archive.evac; Syringe.psi @19:45:22.34 Archive.in :default @19:45:22.41 Archive.home.jog 0 @19:45:22.53 Archive.seek :evac @19:45:23.78 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 78uL (sensed 108uL) @19:45:27.01 -> Archive.home.jog 0; Archive.evac; Syringe.psi @19:45:27.02 Archive.in :default @19:45:27.10 Archive.home.jog 0 @19:45:27.22 Archive.seek :evac @19:45:28.23 Slide::Error in preston -- Archive Syringe (ArS) positionErr at 76uL (sensed 106uL) @19:45:43.03 -> Syringe.stauts @19:45:43.05 NoMethodError in preston -- undefined method `stauts' for Syringe:Class @19:45:47.02 -> Syringe.status {Lysis2 => , Lysis1 => , Air => , Archive => } @19:45:51.97 -> Archive.status @19:46:02.81 -> Archive.status @19:46:30.18 -> Archive.status.position -8108 @19:47:21.18 Session Begun @19:47:24.98 -> exit @19:47:25.00 Session Ended @19:47:57.86 -> Syringe.psi 24.1930627916392 @19:48:09.26 -> BACsampler.evac2waste @19:48:09.27 Handoff.select :waste @19:48:22.97 delay 1:00 @19:49:23.00 Handoff.select :blocked @19:49:47.69 Handoff.select :waste @19:49:47.81 3.2psi at evacEnd @19:49:54.21 -> Syringe.psi 0.079933448052433 @19:50:22.18 -> slots {[44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,5,3] => { Type: :archive_bac, State: :reserved, was: :dry}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :dry}, [59,58,47] => { Type: :unknown, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :lysing, filtered: 330.3, spr: spr23apr1318h.spr.bz2}, [57,56,52..48,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}} @19:50:57.31 -> Cartridge.unlock @19:50:57.32 Gate.power :heat,:OFF @19:50:57.35 Gate.power :cartridge,:OFF @19:50:57.45 PinPort.in :unlocking @19:50:57.52 PinPort.seek :clear @19:50:58.00 Carousel.in :default @19:50:58.12 Carousel.home.seek 60 @19:50:58.43 Slide::Error in preston -- Archive Syringe (ArS) Not yet Homed! @19:51:10.96 -> Archive.home.jog 0 @19:51:10.97 Archive.in :default @19:51:11.05 Archive.home.jog 0 Archive Syringe (ArS) at 105uL @19:51:12.91 -> Cartridge.unlock @19:51:13.00 Carousel.in :default @19:51:13.11 Carousel.home.seek 60 @19:51:13.41 Archive.in :unlocking @19:51:13.54 Archive.seek :clear @19:51:13.58 Air.in :unlocking @19:51:13.73 Air.seek :clear @19:51:13.82 Lysis2.in :unlocking @19:51:13.95 Lysis2.seek :clear @19:51:17.90 Lysis1.in :unlocking @19:51:18.03 Lysis1.seek :clear @19:51:18.07 Plunge.in :unlocking @19:51:18.17 Plunge.seek :clear @19:51:43.96 -> to 45 @19:51:44.19 Carousel.seek 45 @19:51:47.51 Lysis1.in :fast @19:51:47.58 Lysis1.seek :locked @19:51:50.18 Plunge.in :default @19:51:50.29 Plunge.seek :locked @19:51:50.37 Air.in :fast @19:51:50.43 Air.seek :locked @19:51:52.00 Carousel.reconfigure :locked @19:51:52.15 Powering cartridge bus @19:51:55.22 Gate.setIR 1750,5000,0,6 @19:51:55.34 Gate.setIR 0,5000,0,6 @19:51:55.40 Gate.power :cartridge,:OFF @19:51:55.93 Mounted archive Cartridge :archiveNull_bac @19:54:05.22 -> Archive.verify @19:54:05.25 Archive.in :verifyingCalibration @19:54:05.33 Archive.seek home - 8330 counts @19:54:09.74 Archive.jog 0 @19:54:09.90 Archive.jog 1388 @19:54:11.14 Archive.jog 1389 @19:54:12.39 Archive.jog 1388 @19:54:13.63 Archive.jog 1388 @19:54:14.88 Archive.jog 1389 @19:54:16.13 Archive.jog 1388 {-8330 => -4502, -6942 => -4551, -5553 => -4436, -4165 => -4385, -2777 => -4553, -1388 => -4418, 0 => -267} @19:56:36.38 -> Archive.cal! @19:56:36.41 Archive.in :default @19:56:36.49 Archive.home.jog 0 @19:56:36.66 Archive.in :calibrating @19:56:36.73 Archive.jog 11700 @19:56:42.70 Archive.setRawPosition! 50 @19:56:42.74 Archive.seek :home @19:56:43.11 Archive.jog 0 @19:56:43.27 Archive.jog -1388 @19:56:44.52 Archive.jog -1389 @19:56:45.76 Archive.jog -1388 @19:56:47.01 Archive.jog -1388 @19:56:48.27 Archive.jog -1389 @19:56:49.51 Archive.jog -1388 @19:56:50.83 Archive.jog 0 @19:56:50.99 Archive.jog 1388 @19:56:52.24 Archive.jog 1389 @19:56:53.50 Archive.jog 1388 @19:56:54.75 Archive.jog 1388 @19:56:56.00 Archive.jog 1389 @19:56:57.24 Archive.jog 1388 @19:56:58.50 Slide::Error in preston -- observed Archive[:default].maxAbsPosErr of 871 >= 500 @20:01:51.21 Session Begun @20:02:08.62 -> Archive.verify @20:02:08.67 Archive.in :verifyingCalibration @20:02:08.76 Archive.seek home - 8330 counts @20:02:13.18 Archive.jog 0 @20:02:13.35 Archive.jog 1388 @20:02:14.60 Archive.jog 1389 @20:02:15.86 Archive.jog 1388 @20:02:17.55 Archive.jog 1388 @20:02:18.82 Archive.jog 1389 @20:02:20.09 Archive.jog 1388 {-8330 => -4288, -6942 => -4332, -5553 => -4238, -4165 => -4177, -2777 => -4340, -1388 => -4203, 0 => -274} @20:02:44.22 -> Archive.verify @20:02:44.27 Archive.seek home - 8330 counts @20:02:48.71 Archive.jog 0 @20:02:48.88 Archive.jog 1388 @20:02:50.13 Archive.jog 1389 @20:02:51.38 Archive.jog 1388 @20:02:52.64 Archive.jog 1388 @20:02:53.90 Archive.jog 1389 @20:02:55.16 Archive.jog 1388 {-8330 => -438, -6942 => -344, -5553 => -368, -4165 => -90, -2777 => -203, -1388 => -238, 0 => -264} @20:04:02.89 -> Archive.monitorAbsPos @20:04:02.91 Archive.configure #, acceleration=100, deceleration=0, stopRate=0, maxSpeed=800, minSpeed=0, maxPWM=60, minPWM=0, maxSettling=150, maxCurrent=0, stiction=0, friction=23563, hunt=false, brake=false, maxDeltaPress=12, maxInPress=0, maxOutPress=0, minDeltaPress=20678, minInPress=0, minOutPress=0, pressBias=0, pressGain=0, inputDeltaPress=false, externalCurrent=false, debug=false> @20:04:03.12 Archive: absPos=23827, freqShift=-3292, amplitude=3410 @20:04:03.71 Archive: absPos=23824, freqShift=-3318, amplitude=3404 @20:04:04.30 Archive: absPos=23825, freqShift=-3299, amplitude=3407 @20:04:04.89 Archive: absPos=23825, freqShift=-3289, amplitude=3414 @20:04:05.48 Archive: absPos=23825, freqShift=-3283, amplitude=3411 @20:04:06.07 Archive: absPos=23825, freqShift=-3278, amplitude=3412 @20:04:06.67 Archive: absPos=23826, freqShift=-3287, amplitude=3419 @20:04:07.26 Archive: absPos=23824, freqShift=-3290, amplitude=3411 @20:04:07.85 Archive: absPos=23825, freqShift=-3286, amplitude=3422 @20:04:08.45 Archive: absPos=23825, freqShift=-3283, amplitude=3406 @20:04:09.04 Archive: absPos=23826, freqShift=-3307, amplitude=3407 @20:04:09.63 Archive: absPos=23826, freqShift=-3289, amplitude=3407 @20:04:10.22 Archive: absPos=23827, freqShift=-3293, amplitude=3413 @20:04:10.82 Archive: absPos=23825, freqShift=-3291, amplitude=3407 @20:04:11.41 Archive: absPos=23824, freqShift=-3289, amplitude=3409 @20:04:12.00 Archive: absPos=23825, freqShift=-3290, amplitude=3406 @20:04:12.59 Archive: absPos=23826, freqShift=-3312, amplitude=3404 @20:04:13.18 Archive: absPos=23821, freqShift=-3291, amplitude=3427 @20:04:13.77 Archive: absPos=23824, freqShift=-3337, amplitude=3227 @20:04:14.36 Archive: absPos=23827, freqShift=-3350, amplitude=3212 @20:04:14.96 Archive: absPos=23820, freqShift=-3326, amplitude=3265 @20:04:15.55 Archive: absPos=23837, freqShift=-3361, amplitude=3199 @20:04:16.14 Archive: absPos=23834, freqShift=-3316, amplitude=3378 @20:04:16.73 Archive: absPos=23852, freqShift=-3371, amplitude=3181 @20:04:17.32 Archive: absPos=23839, freqShift=-3323, amplitude=3364 @20:04:17.92 Archive: absPos=23850, freqShift=-3348, amplitude=3217 @20:04:18.51 Archive: absPos=23862, freqShift=-3332, amplitude=3233 @20:04:19.11 Archive: absPos=23857, freqShift=-3332, amplitude=3259 @20:04:19.70 Archive: absPos=23848, freqShift=-3327, amplitude=3302 @20:04:20.29 Archive: absPos=23849, freqShift=-3299, amplitude=3415 @20:04:20.88 Archive: absPos=23853, freqShift=-3307, amplitude=3410 @20:04:21.47 Archive: absPos=23849, freqShift=-3304, amplitude=3416 @20:04:22.06 Archive: absPos=23848, freqShift=-3280, amplitude=3426 @20:04:22.65 Archive: absPos=23848, freqShift=-3302, amplitude=3419 @20:04:23.25 Archive: absPos=23848, freqShift=-3288, amplitude=3422 @20:04:23.84 Archive: absPos=23848, freqShift=-3309, amplitude=3413 @20:04:24.44 Archive: absPos=23851, freqShift=-3304, amplitude=3426 @20:04:25.04 Archive: absPos=23846, freqShift=-3294, amplitude=3430 @20:04:25.63 Archive: absPos=23849, freqShift=-3301, amplitude=3426 @20:04:26.22 Archive: absPos=23849, freqShift=-3300, amplitude=3423 @20:04:26.81 Archive: absPos=23851, freqShift=-3312, amplitude=3420 @20:04:27.41 Archive: absPos=23852, freqShift=-3314, amplitude=3416 @20:04:28.00 Archive: absPos=23850, freqShift=-3299, amplitude=3414 @20:04:28.59 Archive: absPos=23852, freqShift=-3309, amplitude=3420 @20:04:29.18 Archive: absPos=23850, freqShift=-3312, amplitude=3423 @20:04:29.77 Archive: absPos=23850, freqShift=-3297, amplitude=3421 @20:04:30.82 Archive: absPos=23847, freqShift=-3289, amplitude=3413 @20:04:31.41 Archive: absPos=23849, freqShift=-3293, amplitude=3416 @20:04:32.00 Archive: absPos=23846, freqShift=-3292, amplitude=3416 @20:04:32.59 Archive: absPos=23851, freqShift=-3304, amplitude=3413 @20:04:33.18 Archive: absPos=23849, freqShift=-3290, amplitude=3414 @20:04:33.78 Archive: absPos=23848, freqShift=-3286, amplitude=3417 @20:04:34.37 Archive: absPos=23848, freqShift=-3294, amplitude=3412 @20:04:34.97 Archive: absPos=23847, freqShift=-3290, amplitude=3421 @20:04:35.57 Archive: absPos=23848, freqShift=-3302, amplitude=3417 @20:04:36.16 Archive: absPos=23847, freqShift=-3282, amplitude=3421 @20:04:36.75 Archive: absPos=23849, freqShift=-3292, amplitude=3421 @20:04:37.34 Archive: absPos=23850, freqShift=-3293, amplitude=3422 @20:04:37.93 Archive: absPos=23848, freqShift=-3290, amplitude=3422 @20:04:38.53 Archive: absPos=23847, freqShift=-3295, amplitude=3422 @20:04:39.12 Archive: absPos=23849, freqShift=-3298, amplitude=3422 @20:04:39.71 Archive: absPos=23849, freqShift=-3300, amplitude=3420 @20:04:40.30 Archive: absPos=23847, freqShift=-3300, amplitude=3419 @20:04:40.90 Archive: absPos=23849, freqShift=-3306, amplitude=3419 @20:04:41.49 Archive: absPos=23851, freqShift=-3298, amplitude=3416 @20:04:42.08 Archive: absPos=23846, freqShift=-3291, amplitude=3424 @20:04:42.67 Archive: absPos=23848, freqShift=-3290, amplitude=3422 @20:04:43.26 Archive: absPos=23847, freqShift=-3307, amplitude=3419 @20:04:43.86 Archive: absPos=23850, freqShift=-3293, amplitude=3419 @20:04:44.45 Archive: absPos=23847, freqShift=-3288, amplitude=3415 @20:04:45.04 Archive: absPos=23850, freqShift=-3288, amplitude=3416 @20:04:45.63 Archive: absPos=23850, freqShift=-3289, amplitude=3412 @20:04:46.22 Archive: absPos=23852, freqShift=-3309, amplitude=3416 @20:04:46.82 Archive: absPos=23853, freqShift=-3304, amplitude=3417 @20:04:47.41 Archive: absPos=23852, freqShift=-3312, amplitude=3411 @20:04:48.00 Archive: absPos=23847, freqShift=-3296, amplitude=3414 @20:04:48.59 Archive: absPos=23848, freqShift=-3295, amplitude=3417 @20:04:49.18 Archive: absPos=23848, freqShift=-3294, amplitude=3425 @20:04:49.73 UserAbort in brent -- User Abort!! @20:04:58.63 -> Archive.cal! @20:04:58.66 Archive.in :default @20:04:58.77 Archive.home.jog 0 @20:04:58.95 Archive.in :calibrating @20:04:59.03 Archive.jog 11700 @20:05:05.00 Archive.setRawPosition! 50 @20:05:05.07 Archive.seek :home @20:05:05.44 Archive.jog 0 @20:05:05.61 Archive.jog -1388 @20:05:06.86 Archive.jog -1389 @20:05:08.16 Archive.jog -1388 @20:05:09.41 Archive.jog -1388 @20:05:10.67 Archive.jog -1389 @20:05:11.92 Archive.jog -1388 @20:05:13.26 Archive.jog 0 @20:05:13.43 Archive.jog 1388 @20:05:14.68 Archive.jog 1389 @20:05:16.31 Archive.jog 1388 @20:05:17.64 Archive.jog 1388 @20:05:18.90 Archive.jog 1389 @20:05:20.15 Archive.jog 1388 @20:05:21.43 Archive[:default].absSkew [-8330,-6942,-5553,-4165,-2777,-1388,0], [-18312,-11409,-4358,2384,9508,16577,23679] @20:05:21.44 Archive.in :default {-8330 => 1, -6942 => 24, -5553 => 22, -4165 => 21, -2777 => 27, -1388 => 18, 0 => 12, absSkew: 5.037069(absPosCounts/step)+23560, backlash: 17.8571428571429} @20:05:46.70 -> Archive.verify @20:05:46.75 Archive.in :verifyingCalibration @20:05:46.84 Archive.seek home - 8330 counts @20:05:51.26 Archive.jog 0 @20:05:51.44 Archive.jog 1388 @20:05:52.70 Archive.jog 1389 @20:05:53.96 Archive.jog 1388 @20:05:55.21 Archive.jog 1388 @20:05:56.47 Archive.jog 1389 @20:05:57.74 Archive.jog 1388 {-8330 => -87, -6942 => 15, -5553 => -36, -4165 => 209, -2777 => 77, -1388 => -5, 0 => -123} @20:06:05.95 -> Archive.verify @20:06:06.00 Archive.seek home - 8330 counts @20:06:10.44 Archive.jog 0 @20:06:10.61 Archive.jog 1388 @20:06:11.86 Archive.jog 1389 @20:06:13.12 Archive.jog 1388 @20:06:14.37 Archive.jog 1388 @20:06:15.63 Archive.jog 1389 @20:06:16.88 Archive.jog 1388 {-8330 => -84, -6942 => 18, -5553 => -39, -4165 => 211, -2777 => 78, -1388 => -3, 0 => -122} @20:06:45.12 -> to 60 @20:06:45.33 Carousel.in :default @20:06:45.46 Carousel.home.seek 45 @20:06:45.87 Air.in :unlocking @20:06:46.01 Air.seek :clear @20:06:47.37 Lysis1.in :unlocking @20:06:47.52 Lysis1.seek :clear @20:06:47.59 Plunge.in :unlocking @20:06:47.70 Plunge.seek :clear @20:06:48.91 Carousel.seek 60 @20:06:52.69 Lysis1.in :fast @20:06:52.78 Lysis1.seek :locked @20:06:55.39 Plunge.in :default @20:06:55.50 Plunge.seek :locked @20:06:55.62 Air.in :fast @20:06:55.70 Air.seek :locked @20:06:57.27 Carousel.reconfigure :locked @20:06:57.43 Powering cartridge bus @20:07:00.04 Gate.setIR 1750,5000,0,6 @20:07:00.17 Gate.setIR 0,5000,0,6 @20:07:00.24 PuckHeater.configure :default @20:07:00.31 Gate.power :heat,:ON,limit to 5000mA @20:07:00.95 Mounted lysis Cartridge board#Q05062 @20:07:01.00 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @20:07:57.26 -> BACsampler.prepareLGcart @20:07:57.27 Handoff.select :waste @20:08:03.88 Syringe tare is 17% of full scale @20:08:03.91 PinPort.in :locking @20:08:04.01 PinPort.seek :locked @20:08:04.50 Archive.in :fast @20:08:04.56 Lysis2.in :fast @20:08:04.65 Lysis1.seek 1350uL @20:08:04.72 Archive.seek :full @20:08:04.81 Lysis2.seek :full Archive Syringe (ArS) full @20:08:28.46 -> Handoff Handoff Valve::Manifold selects waste @20:08:31.58 -> Air.evac @20:08:31.60 Air.in :default @20:08:31.71 Air.seek :evac Air Syringe (AirS) at evac @20:08:49.48 -> Handoff.blocked @20:08:49.49 Handoff.select :blocked Handoff Valve::Manifold selects blocked @20:08:58.56 -> Archive.pumpTo 50 @20:08:58.58 Archive syringe pumping up to 8 strokes to reach 50psi @20:08:58.60 Archive stroke #1 of 8 @20:08:58.73 Archive.in :pressurize @20:08:58.81 Archive.seek :pressurized @20:09:11.32 0.2psi after pressurize @20:09:16.89 0.2psi after pressurize (stable) @20:09:16.90 Archive.in :compress @20:09:17.01 Archive.seek :empty @20:09:31.63 2.1psi after compress @20:10:07.71 19.0psi after compress (stable) @20:10:07.74 Archive.in :gulp @20:10:07.84 Archive.seek :topVent @20:10:14.09 19.9psi after gulp @20:10:19.66 20.5psi after gulp (stable) @20:10:19.67 Archive stroke #2 of 8 @20:10:19.79 Archive.in :pressurize @20:10:19.87 Archive.seek :pressurized @20:10:32.36 21.3psi after pressurize @20:10:37.93 21.5psi after pressurize (stable) @20:10:37.95 Archive.in :compress @20:10:38.05 Archive.seek :empty @20:10:52.67 23.6psi after compress @20:11:25.24 33.0psi after compress (stable) @20:11:25.25 Archive.in :gulp @20:11:25.45 Archive.seek :topVent @20:11:31.71 33.9psi after gulp @20:11:37.28 34.4psi after gulp (stable) @20:11:37.29 Archive stroke #3 of 8 @20:11:37.41 Archive.in :pressurize @20:11:37.48 Archive.seek :pressurized @20:11:49.97 35.2psi after pressurize @20:11:55.55 35.5psi after pressurize (stable) @20:11:55.94 Archive.in :compress @20:11:56.12 Archive.seek :empty @20:12:10.73 37.2psi after compress @20:12:13.54 -> hidelog Log display suppressed @20:12:27.51 -> exit @20:12:27.53 Session Ended @20:12:44.31 46.1psi after compress (stable) @20:12:44.33 Archive.in :gulp @20:12:44.41 Archive.seek :topVent @20:12:50.64 47.1psi after gulp @20:12:56.22 47.5psi after gulp (stable) @20:12:56.23 Archive stroke #4 of 8 @20:12:56.32 Archive.in :pressurize @20:12:56.39 Archive.seek :pressurized @20:13:08.87 48.3psi after pressurize @20:13:14.45 48.5psi after pressurize (stable) @20:13:14.47 Archive.in :compress @20:13:14.54 Archive.seek :empty @20:13:29.15 49.7psi after compress @20:13:29.91 Reached 50.1 of 50.0psi after pumping 6920uL (4.00 strokes) @20:13:29.92 Archive.in :default @20:13:30.00 Archive.hold 6920.0 @20:13:41.87 -> BACsampler.evac2waste @20:13:41.88 Handoff.select :waste @20:13:49.08 delay 1:00 @20:14:49.11 Handoff.select :blocked @20:15:11.78 Handoff.select :waste @20:15:11.91 2.3psi at evacEnd @20:18:40.89 -> Handoff Handoff Valve::Manifold selects waste @20:18:46.63 -> Handoff.blocked @20:18:46.64 Handoff.select :blocked Handoff Valve::Manifold selects blocked @20:18:51.46 -> Archive.pumpTo 50 @20:18:51.49 Archive syringe pumping up to 8 strokes to reach 50psi @20:18:51.50 Archive stroke #1 of 8 @20:18:51.60 Archive.in :compress @20:18:51.68 Archive.seek :empty @20:18:51.81 -0.1psi after compress @20:19:02.38 0.0psi after compress (stable) @20:19:02.40 Archive.in :gulp @20:19:02.48 Archive.seek :topVent @20:19:08.71 0.1psi after gulp @20:19:14.29 0.1psi after gulp (stable) @20:19:14.30 Archive stroke #2 of 8 @20:19:14.39 Archive.in :pressurize @20:19:14.47 Archive.seek :pressurized @20:19:26.96 0.4psi after pressurize @20:19:49.53 5.1psi after pressurize (stable) @20:19:49.55 Archive.in :compress @20:19:49.62 Archive.seek :empty @20:20:04.23 11.1psi after compress @20:20:17.30 14.4psi after compress (stable) @20:20:17.32 Archive.in :gulp @20:20:17.39 Archive.seek :topVent @20:20:23.63 14.6psi after gulp @20:20:29.20 14.6psi after gulp (stable) @20:20:29.21 Archive stroke #3 of 8 @20:20:29.30 Archive.in :pressurize @20:20:29.37 Archive.seek :pressurized @20:20:41.85 14.5psi after pressurize @20:20:56.43 16.7psi after pressurize (stable) @20:20:56.44 Archive.in :compress @20:20:56.52 Archive.seek :empty @20:21:11.12 25.0psi after compress @20:21:23.70 28.2psi after compress (stable) @20:21:23.71 Archive.in :gulp @20:21:23.80 Archive.seek :topVent @20:21:30.04 28.5psi after gulp @20:21:35.61 28.4psi after gulp (stable) @20:21:35.62 Archive stroke #4 of 8 @20:21:35.71 Archive.in :pressurize @20:21:35.78 Archive.seek :pressurized @20:21:48.29 28.2psi after pressurize @20:21:53.87 28.6psi after pressurize (stable) @20:21:53.88 Archive.in :compress @20:21:53.96 Archive.seek :empty @20:22:08.57 38.4psi after compress @20:22:20.64 41.6psi after compress (stable) @20:22:20.66 Archive.in :gulp @20:22:20.73 Archive.seek :topVent @20:22:26.97 41.8psi after gulp @20:22:32.54 41.8psi after gulp (stable) @20:22:32.55 Archive stroke #5 of 8 @20:22:32.64 Archive.in :pressurize @20:22:32.71 Archive.seek :pressurized @20:22:45.20 41.6psi after pressurize @20:22:50.77 41.6psi after pressurize (stable) @20:22:50.79 Archive.in :compress @20:22:50.86 Archive.seek :empty @20:23:04.70 Reached 50.1 of 50.0psi after pumping 8633uL (4.99 strokes) @20:23:04.71 Archive.in :default @20:23:04.78 Archive.hold 8632.85268160217 @20:23:16.10 -> BACsampler.evac2waste @20:23:16.12 Handoff.select :waste @20:23:29.81 delay 1:00 @20:24:29.83 Handoff.select :blocked @20:24:52.51 Handoff.select :waste @20:24:52.64 2.0psi at evacEnd @20:25:04.97 -> Air.fVent @20:25:04.99 Air.seek :fVent Air Syringe (AirS) at fVent @20:26:57.90 -> BACsampler.deliverLysis @20:26:57.99 Lysis1.in :slow @20:26:58.06 Lysis1.seek 1050uL @20:27:28.08 delay 5 seconds @20:27:33.10 Handoff.select :blocked @20:27:33.28 Lysis1.seek 450uL @20:28:33.22 delay 3 seconds @20:28:36.30 Air.in mix with 1.40048 parts Lysis1 @20:28:36.93 Air.seek :evac @20:28:36.99 Lysis1.seek 300uL @20:28:52.08 Lysis1.seek :empty @20:29:22.17 Lysis buffer delivered @13.5psi @20:29:59.35 -> padProfile @20:29:59.37 Polling Can every 2:00 @20:29:59.92 PuckHeater.configure :default #ambient 22.3C @20:29:59.98 PuckHeater.seek 79.7C @20:30:52.55 PuckHeater.hold 2:00,79.7C @20:32:52.49 Polling Can every 2:00 @20:32:53.03 PuckHeater.configure :default #ambient 22.3C @20:32:53.09 PuckHeater.hold 30 seconds,78.7C @20:33:23.25 Polling Can every 2:00 @20:33:23.79 PuckHeater.configure :default #ambient 22.3C @20:33:23.84 PuckHeater.hold 30 seconds,78.2C @20:33:54.00 Polling Can every 2:00 @20:33:54.55 PuckHeater.hold 10:00,77.6C Puck Heater at 77.9C @20:44:26.84 -> BACsampler.recoverLysate @20:44:26.86 Archive syringe pumping up to 5 strokes to reach 40psi @20:44:26.88 Archive stroke #1 of 5 @20:44:26.97 Archive.in :compress @20:44:27.04 Archive.seek :empty @20:44:27.98 14.9psi after compress @20:44:38.55 15.1psi after compress (stable) @20:44:38.57 Archive.in :gulp @20:44:38.65 Archive.seek :topVent @20:44:44.90 15.0psi after gulp @20:44:50.47 14.9psi after gulp (stable) @20:44:50.48 Archive stroke #2 of 5 @20:44:50.58 Archive.in :pressurize @20:44:50.65 Archive.seek :pressurized @20:45:03.14 15.1psi after pressurize @20:45:38.72 26.6psi after pressurize (stable) @20:45:38.73 Archive.in :compress @20:45:38.81 Archive.seek :empty @20:45:50.15 Reached 40.4 of 40.0psi after pumping 3389uL (1.96 strokes) @20:45:50.17 Archive.in :default @20:45:50.24 Archive.hold @20:45:50.32 delay 2 seconds @20:45:52.34 Archive.in :fast @20:45:52.41 Archive.seek :full @20:45:59.32 Lysis1.seek 138uL @20:46:13.18 delay 1 second @20:46:14.21 Archive.seek :full @20:46:14.25 Lysis1.seek :empty @20:46:28.17 Handoff.select :blocked @20:46:28.75 Initial air pressure @44.0psi @20:46:28.81 Lysis1.in :extract @20:46:28.91 Lysis1.seek 600uL,nil @20:46:32.98 Archive syringe pumping up to 6 strokes to reach 25psi @20:46:32.99 Archive stroke #1 of 6 @20:46:33.09 Archive.in :pressurize @20:46:33.18 Archive.seek :pressurized @20:46:45.67 13.7psi after pressurize @20:46:55.97 Archive.in :default @20:46:56.02 Archive.hold @20:46:56.15 Initial 600uL lysate @15.0psi @20:46:56.16 Handoff.select :blocked @20:46:56.27 Initial air pressure @15.1psi @20:46:56.34 Lysis1.seek 825uL,nil @20:46:56.43 Archive syringe pumping up to 6 strokes to reach 17psi @20:46:56.44 Archive stroke #1 of 6 @20:46:56.58 Archive.in :compress @20:46:56.66 Archive.seek :empty @20:47:08.46 Archive.in :default @20:47:08.52 Archive.hold @20:47:08.73 Recovered 825uL lysate @14.5psi @20:47:08.75 Air.in :halfSpeed @20:47:08.83 Air.seek :fullVent @20:47:11.03 Puck Top Vented, bottom @8.5psi @20:47:11.04 Handoff.select :waste @20:47:11.17 delay 5 seconds @20:47:16.24 Handoff open to waste, bottom @0.1psi @20:47:16.25 Air.in :default @20:47:16.33 Air.seek :evac 825 @20:47:25.82 -> Air Air Syringe (AirS) at evac @20:47:42.20 -> Cmd.stop @20:47:42.30 Handoff.select :close @20:47:42.43 Gate.power :heat,:OFF @20:47:42.45 Gate.power :cartridge,:OFF @20:47:42.54 PinPort.in :unlocking @20:47:42.61 PinPort.seek :clear @20:47:43.09 Carousel.in :default @20:47:43.21 Carousel.home.seek 60 @20:47:43.50 Plunge.seek :locked @20:47:43.65 Archive.in :unlocking @20:47:43.79 Archive.seek :clear @20:47:43.82 Air.in :unlocking @20:47:44.00 Air.seek :clear @20:47:44.04 Lysis1.in :unlocking @20:47:44.19 Lysis1.seek :clear @20:47:44.29 Lysis2.in :unlocking @20:47:44.37 Lysis2.seek :clear @20:47:48.34 Gate.power :motor,:OFF @20:47:48.36 Gate.power :core,:OFF @20:47:48.39 Gate.power :analytic1,:OFF @20:47:48.41 Gate.power :analytic2,:OFF @20:47:48.43 Gate.power :analytic3,:OFF @20:47:48.45 Gate.power :main,:OFF @20:47:48.48 Safely stopped and ready to power off :STOPPED @20:49:01.87 -> slots {[44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,5,3] => { Type: :archive_bac, State: :reserved, was: :dry}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :dry}, [59,58,47] => { Type: :unknown, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :lysing, filtered: 330.3, lysed: 825, spr: spr23apr1318h.spr.bz2}, [57,56,52..48,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}} @20:49:05.43 -> Cartridge.dry [46] @20:49:13.58 -> MainThread.exit # @20:49:13.66 Session Ended @20:49:27.65PDT13-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @20:49:27.67 >BREAK< ESP3 Gateway v1.42 for cartridge bus @20:49:30.15 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @20:49:30.62 Sea pressure (Abs) tare is 6% of full scale @20:49:34.72 Polling Can every 2:00 @20:49:34.76 Loading /home/esp/esp2/bin/processAll mission @20:49:35.69 Sea pressure (Abs) tare is 6% of full scale @20:49:35.70 Sampling default goal volumes @20:49:35.73 GoodNews.email "Cartridge group: dry\n\n",Subject: "Begun processAll --mail" @20:49:36.25
Gate.power :main,:ON,limit to 5000mA @20:49:36.27 Gate.power :analytic1,:ON,limit to 5000mA @20:49:36.28 Gate.power :analytic2,:ON,limit to 5000mA @20:49:36.29 Gate.power :analytic3,:ON,limit to 5000mA @20:49:36.42 Powering core bus @20:49:38.69 SPR Module 1, FW Rev. B, FPGA Rev. C @20:49:39.14
Archive.configure :default @20:49:39.22 Air.configure :default @20:49:39.27 Lysis1.configure :default @20:49:39.32 Lysis2.configure :default @20:49:39.40 Plunge.configure :default @20:49:39.47 SamplePump.configure :default @20:49:39.55 Toroid.configure :default @20:49:39.62 Carousel.configure :default @20:49:39.70 Pressure.configure :default @20:49:39.78 ExtraPump.configure :default @20:49:39.83 PinPort.configure :default @20:49:39.88 All elf controllers running firmware version 4.83 @20:49:39.90 Gate.power :motor,:ON,limit to 5000mA @20:49:40.22 Archive.home.jog 0 @20:49:40.60 Carousel.home.jog 0 @20:49:40.64 Toroid.home.jog 0 @20:49:40.66 Lysis1.home.jog 0 @20:49:40.69 Lysis2.home.jog 0 @20:49:40.73 PinPort.home.jog 0 @20:49:40.75 Plunge.home.jog 0 @20:49:40.78 Air.home.jog 0 @20:49:41.26 Archive.in :homing @20:49:41.55 Archive.jog 1382 @20:49:41.59 PinPort.in :homing @20:49:41.61 Toroid.in :homing @20:49:41.64 Plunge.in :homing @20:49:41.67 Lysis2.in :homing @20:49:41.70 Air.in :homing @20:49:41.73 Lysis1.in :homing @20:49:41.94 PinPort.jog -107 @20:49:41.99 Toroid.jog 626 @20:49:42.02 Plunge.jog 1531 @20:49:42.04 Lysis2.jog 1387 @20:49:42.05 Lysis1.jog 1403 @20:49:42.09 Air.jog 1388 @20:49:42.30 PinPort.setRawPosition! 0 @20:49:42.34 PinPort.seek :home @20:49:42.41 PinPort.in :default @20:49:42.90 Archive.setRawPosition! 50 @20:49:42.94 Archive.seek :home @20:49:43.20 Archive.in :default @20:49:43.34 Lysis1.setRawPosition! 50 @20:49:43.41 Plunge.setRawPosition! 50 @20:49:43.45 Lysis2.setRawPosition! 50 @20:49:43.46 Air.setRawPosition! 50 @20:49:43.48 Lysis1.seek :home @20:49:43.60 Lysis2.seek :home @20:49:43.64 Plunge.seek :home @20:49:43.67 Air.seek :home @20:49:44.22 Toroid.setRawPosition! 0 @20:49:44.25 Lysis1.in :default @20:49:44.32 Toroid.seek :home @20:49:44.47 Plunge.in :default @20:49:44.51 Lysis2.in :default @20:49:44.54 Toroid.in :default @20:49:44.56 Air.in :default @20:49:44.97
Toroid.seek :clear @20:49:46.58 Carousel.in :default @20:49:46.68 Carousel.home.seek 60 @20:49:46.92 Selecting Cartridge 46 @20:49:47.14 Carousel.seek 46 @20:49:50.27 Lysis1.in :fast @20:49:50.32 Lysis1.seek :locked @20:49:52.91 Plunge.seek :locked @20:49:53.02 Air.in :fast @20:49:53.08 Air.seek :locked @20:49:54.64
Carousel.reconfigure :locked @20:49:54.77 Powering cartridge bus @20:49:57.38 Gate.setIR 1750,5000,0,6 @20:49:57.49 Gate.setIR 0,5000,0,6 @20:49:57.54 Gate.power :cartridge,:OFF @20:49:58.07 Mounted archive Cartridge @20:49:58.18 GoodNews.email "Filtering up to 1000ml",Subject: "Sampling archive_bac cartridge in slot 46" @20:49:58.46 Preparing to filter cartridge 46 @20:49:58.73 Priming sample loop w/100ml, bypass w/10ml @20:49:58.75 SamplePump.setPosition! 0ml @20:49:58.80 Bypass.open @20:50:00.27 Intake.open @20:50:01.01 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @20:50:03.58 Vacuum tare is 60% of full scale @20:50:04.00 Pressure.in :default @20:50:04.12 Pressure.hold @20:50:04.30 SamplePump.in :prime @20:50:04.36 SamplePump.seek 10ml,59 seconds @20:50:13.08 Pressure.stop @20:50:13.12 SamplePump.setPosition! 0ml @20:50:13.16 Exhaust.open @20:50:14.61 Bypass.close @20:50:16.09 Pressure.in :default @20:50:16.14 Pressure.hold @20:50:16.36 SamplePump.in :prime @20:50:16.42 SamplePump.seek 100ml,5:16 @20:51:42.01 Pressure.stop @20:51:42.07 Bypass.open @20:51:42.73 Intake.close @20:51:43.48 Toroid.in :hold @20:51:43.53 Toroid.seek :crack @20:51:45.05 Toroid.seek :cartridge @20:51:46.14 Toroid.in :default @20:51:46.19 Toroid.seek :clear @20:51:48.14 Pressurizing cartridge to 26psi above 15.1psia @20:51:48.15 SamplePump.setPosition! 0ml @20:51:48.18 Intake.open @20:51:48.83 Bypass.close @20:51:49.48 Exhaust.close @20:51:50.26 Pressure.in :default @20:51:50.31 Pressure.hold @20:51:50.48 SamplePump.in :charge @20:51:50.53 SamplePump.seek 2.3ml,42 seconds @20:51:56.62 Pressure.stop @20:51:56.67 Intake.close @20:51:57.37 Pressurized cartridge with 0.174ml to 26.3psi @20:51:57.39 Isolated @39.1psia (24.0psi) @20:51:57.40 Verifying cartridge holds at least 0.65..0.85 of 39.1psia @20:51:57.41 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @20:52:02.06 Sampling 1000.0ml in 1:25:20 @20:52:02.07 SamplePump.setPosition! 0ml @20:52:02.11 Exhaust.open @20:52:02.84 Intake.open @20:52:04.00 Pressure.in :default @20:52:04.05 Pressure.hold @20:52:04.16 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @20:52:04.30 SamplePump.in :sample @20:52:04.35 SamplePump.seek 1000ml,1:23:51 @20:52:07.05 Cartridge 46 filtering 1/1000ml at 0.54ml/s with 25.4psi vacuum @20:53:07.05 Cartridge 46 filtering 31/1000ml at 0.51ml/s with 23.0psi vacuum @20:54:07.05 Cartridge 46 filtering 59/1000ml at 0.46ml/s with 27.7psi vacuum @20:55:07.05 Cartridge 46 filtering 87/1000ml at 0.44ml/s with 17.5psi vacuum @20:56:07.05 Cartridge 46 filtering 113/1000ml at 0.45ml/s with 23.1psi vacuum 2.0m deep (17.6psia) @20:57:07.05 Cartridge 46 filtering 137/1000ml at 0.41ml/s with 24.7psi vacuum @20:58:07.06 Cartridge 46 filtering 160/1000ml at 0.36ml/s with 25.3psi vacuum @20:59:07.05 Cartridge 46 filtering 181/1000ml at 0.38ml/s with 24.1psi vacuum 2.3m deep (18.1psia) @21:00:07.05 Cartridge 46 filtering 201/1000ml at 0.31ml/s with 25.1psi vacuum @21:01:07.05 Cartridge 46 filtering 219/1000ml at 0.30ml/s with 25.8psi vacuum @21:02:07.05 Cartridge 46 filtering 236/1000ml at 0.27ml/s with 25.6psi vacuum @21:03:07.05 Cartridge 46 filtering 252/1000ml at 0.29ml/s with 27.8psi vacuum @21:04:07.05 Cartridge 46 filtering 268/1000ml at 0.22ml/s with 24.6psi vacuum @21:05:07.05 Cartridge 46 filtering 282/1000ml at 0.22ml/s with 23.4psi vacuum @21:06:07.05 Cartridge 46 filtering 296/1000ml at 0.24ml/s with 11.2psi vacuum 2.6m deep (18.5psia) @21:07:07.06 Cartridge 46 filtering 309/1000ml at 0.20ml/s with 26.2psi vacuum @21:07:38.95 Flow averaged 0.34ml/s over 15:35 @21:07:38.99 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @21:07:39.56 Relieved excess vacuum in .6 seconds @21:07:39.58 SamplePump.seek 1000ml,57:30 @21:07:45.67 Flow estimate reduced to 0.27ml/s @21:07:45.70 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @21:07:46.28 Relieved excess vacuum in .6 seconds @21:07:46.30 SamplePump.seek 1000ml,57:24 @21:07:52.74 Flow estimate reduced to 0.22ml/s @21:07:52.77 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @21:07:53.35 Relieved excess vacuum in .6 seconds @21:07:53.37 SamplePump.seek 1000ml,57:17 @21:07:56.68 Flow estimate reduced to 0.17ml/s @21:07:56.70 Intake.close @21:07:56.76 Exhaust.close @21:07:56.94 Toroid.in :hold @21:07:57.00 Toroid.seek :bypass @21:07:58.62 Toroid.in :default @21:07:58.68 Toroid.seek :clear @21:08:00.63 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @21:08:00.72 Pressure.stop @21:08:00.89 Sampled 319.3ml @21:08:01.28 GoodNews.email "After filtering 319.3ml",Subject: "Processing archive_bac cartridge in slot 46" @21:08:01.76 Plunge.seek :reagent @21:08:02.56 Sample loop closed. Ready to Deliver Reagents @21:08:02.58 Archive.seek :sealed @21:08:06.49 Archive.seek :evac @21:08:20.52 delay 1:00 @21:09:20.55 Archive.seek :clear @21:09:38.03 RNAlater soak for 10:00 @21:19:38.06 Archive.seek :tVent @21:19:40.49 delay 1 second @21:19:41.51 Archive.seek :evac @21:19:57.03 delay 10 seconds @21:20:07.05 Sample preserved and evacuated @21:20:07.26
Carousel.in :default @21:20:07.36 Carousel.home.seek 46 @21:20:07.65 Plunge.seek :locked @21:20:08.50 Archive.in :unlocking @21:20:08.62 Archive.seek :clear @21:20:08.67 Air.in :unlocking @21:20:08.79 Air.seek :clear @21:20:08.88 Lysis1.in :unlocking @21:20:09.01 Lysis1.seek :clear @21:20:13.23
Gate.power :motor,:OFF @21:20:13.24 Gate.power :core,:OFF @21:20:13.25 Gate.power :analytic1,:OFF @21:20:13.26 Gate.power :analytic2,:OFF @21:20:13.27 Gate.power :analytic3,:OFF @21:20:13.28 Gate.power :main,:OFF @21:20:13.29 Safely stopped and ready to power off @21:20:13.70 Cartridge::None in MAIN -- Not enough dry cartridges remain @23:14:01.60PDT13-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @23:14:01.62 >BREAK< ESP3 Gateway v1.42 for cartridge bus @23:14:04.20 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @23:14:04.66 Sea pressure (Abs) tare is 6% of full scale @23:14:08.76 Polling Can every 2:00 @23:14:08.80 Loading /home/esp/esp2/bin/processAll mission @23:14:08.98 Sea pressure (Abs) tare is 6% of full scale @23:14:08.99 Sampling default goal volumes @23:14:09.00 Cartridge group: dry @23:14:09.05 Gate.power :main,:ON,limit to 5000mA @23:14:09.06 Gate.power :analytic1,:ON,limit to 5000mA @23:14:09.07 Gate.power :analytic2,:ON,limit to 5000mA @23:14:09.08 Gate.power :analytic3,:ON,limit to 5000mA @23:14:09.19 Powering core bus @23:14:11.48 SPR Module 1, FW Rev. B, FPGA Rev. C @23:14:11.89
Archive.configure :default @23:14:11.96 Air.configure :default @23:14:12.01 Lysis1.configure :default @23:14:12.07 Lysis2.configure :default @23:14:12.14 Plunge.configure :default @23:14:12.22 SamplePump.configure :default @23:14:12.64 Toroid.configure :default @23:14:12.72 Carousel.configure :default @23:14:12.79 Pressure.configure :default @23:14:12.86 ExtraPump.configure :default @23:14:12.91 PinPort.configure :default @23:14:12.96 All elf controllers running firmware version 4.83 @23:14:12.98 Gate.power :motor,:ON,limit to 5000mA @23:14:13.26 Archive.home.jog 0 @23:14:13.36 Air.home.jog 0 @23:14:13.48 Lysis1.home.jog 0 @23:14:13.58 Lysis2.home.jog 0 @23:14:13.68 Plunge.home.jog 0 @23:14:13.75 Toroid.home.jog 0 @23:14:13.85 Carousel.home.jog 0 @23:14:13.93 PinPort.home.jog 0 @23:14:14.32 Air.in :homing @23:14:14.37 Archive.in :homing @23:14:14.55 Archive.jog 1378 @23:14:14.58 Air.jog 1988 @23:14:14.79 Toroid.in :homing @23:14:14.83 Plunge.in :homing @23:14:14.86 Lysis1.in :homing @23:14:14.89 Lysis2.in :homing @23:14:15.00 Toroid.jog 633 @23:14:15.05 Plunge.jog 1532 @23:14:15.09 Lysis2.jog 1385 @23:14:15.12 Lysis1.jog 2139 @23:14:15.27 PinPort.in :homing @23:14:15.32 PinPort.jog -107 @23:14:15.53 PinPort.setRawPosition! 0 @23:14:15.57 PinPort.seek :home @23:14:15.63 PinPort.in :default @23:14:15.74 Archive.setRawPosition! 50 @23:14:15.78 Archive.seek :home @23:14:16.04 Archive.in :default @23:14:16.10 Air.setRawPosition! 50 @23:14:16.15 Air.seek :home @23:14:16.32 Lysis2.setRawPosition! 50 @23:14:16.38 Lysis2.seek :home @23:14:16.41 Plunge.setRawPosition! 50 @23:14:16.49 Plunge.seek :home @23:14:16.56 Air.in :default @23:14:16.69 Lysis1.setRawPosition! 50 @23:14:16.71 Lysis2.in :default @23:14:16.79 Lysis1.seek :home @23:14:16.88 Plunge.in :default @23:14:17.07 Lysis1.in :default @23:14:17.19 Toroid.setRawPosition! 0 @23:14:17.23 Toroid.seek :home @23:14:17.30 Toroid.in :default @23:14:17.39
Toroid.seek :clear @23:14:19.00 Carousel.in :default @23:14:19.10 Carousel.home.seek 45 @23:14:19.33 Selecting Cartridge 57 @23:14:19.55 Carousel.seek 57 @23:14:22.62 Lysis1.in :fast @23:14:22.67 Lysis1.seek :locked @23:14:25.25 Plunge.seek :locked @23:14:25.37 Air.in :fast @23:14:25.42 Air.seek :locked @23:14:26.98
Carousel.reconfigure :locked @23:14:27.10 Powering cartridge bus @23:14:29.71 Gate.setIR 1750,5000,0,6 @23:14:29.83 Gate.setIR 0,5000,0,6 @23:14:29.88 PuckHeater.configure :default @23:14:29.92 Gate.power :heat,:ON,limit to 5000mA @23:14:30.60 Mounted lysis Cartridge board#Q05080 @23:14:30.62 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:14:30.86 Preparing to filter cartridge 57 @23:14:31.09 Priming sample loop w/100ml, bypass w/10ml @23:14:31.11 SamplePump.setPosition! 0ml @23:14:31.14 Bypass.open @23:14:32.56 Intake.open @23:14:33.26 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:14:35.84 Vacuum tare is 60% of full scale @23:14:35.95 Pressure.in :default @23:14:36.00 Pressure.hold @23:14:36.17 SamplePump.in :prime @23:14:36.23 SamplePump.seek 10ml,59 seconds @23:14:44.95 Pressure.stop @23:14:44.98 SamplePump.setPosition! 0ml @23:14:45.02 Exhaust.open @23:14:46.45 Bypass.close @23:14:47.92 Pressure.in :default @23:14:47.97 Pressure.hold @23:14:48.18 SamplePump.in :prime @23:14:48.24 SamplePump.seek 100ml,5:16 @23:16:15.52 Pressure.stop @23:16:15.58 Bypass.open @23:16:16.26 Intake.close @23:16:16.97 Toroid.in :hold @23:16:17.32 Toroid.seek :crack @23:16:18.91 Toroid.seek :cartridge @23:16:20.00 Toroid.in :default @23:16:20.05 Toroid.seek :clear @23:16:22.00 Pressurizing cartridge to 26psi above 15.1psia @23:16:22.02 SamplePump.setPosition! 0ml @23:16:22.05 Intake.open @23:16:22.69 Bypass.close @23:16:23.36 Exhaust.close @23:16:24.17 Pressure.in :default @23:16:24.22 Pressure.hold @23:16:24.38 SamplePump.in :charge @23:16:24.44 SamplePump.seek 2.3ml,42 seconds @23:16:38.93 Pressure.stop @23:16:38.97 Intake.close @23:16:39.66 Pressurized cartridge with 1.004ml to 26.2psi @23:16:39.68 Isolated @38.7psia (23.6psi) @23:16:39.69 Verifying cartridge holds at least 0.65..0.85 of 38.7psia @23:16:39.71 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:16:44.78 Cartridge pressure fell 11% to 34.5psia @23:16:44.85 Sampling 1000.0ml in 1:25:20 @23:16:44.87 SamplePump.setPosition! 0ml @23:16:44.91 Exhaust.open @23:16:45.63 Intake.open @23:16:46.41 Pressure.in :default @23:16:46.46 Pressure.hold @23:16:46.57 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @23:16:46.70 SamplePump.in :sample @23:16:46.76 SamplePump.seek 1000ml,1:23:51 @23:16:49.45 Cartridge 57 filtering 1/1000ml at 0.47ml/s with 20.7psi vacuum @23:17:49.46 Cartridge 57 filtering 29/1000ml at 0.45ml/s with 23.9psi vacuum @23:18:49.46 Cartridge 57 filtering 55/1000ml at 0.43ml/s with 25.5psi vacuum @23:19:49.46 Cartridge 57 filtering 80/1000ml at 0.42ml/s with 24.6psi vacuum @23:20:49.46 Cartridge 57 filtering 104/1000ml at 0.39ml/s with 22.5psi vacuum @23:21:49.46 Cartridge 57 filtering 127/1000ml at 0.38ml/s with 23.0psi vacuum @23:22:49.46 Cartridge 57 filtering 148/1000ml at 0.35ml/s with 20.6psi vacuum @23:23:49.79 Cartridge 57 filtering 169/1000ml at 0.33ml/s with 25.1psi vacuum @23:24:49.46 Cartridge 57 filtering 188/1000ml at 0.31ml/s with 22.9psi vacuum @23:25:49.47 Cartridge 57 filtering 207/1000ml at 0.31ml/s with 24.0psi vacuum @23:26:49.47 Cartridge 57 filtering 224/1000ml at 0.30ml/s with 24.0psi vacuum @23:27:49.47 Cartridge 57 filtering 241/1000ml at 0.28ml/s with 25.1psi vacuum @23:28:49.46 Cartridge 57 filtering 257/1000ml at 0.24ml/s with 24.9psi vacuum @23:29:49.47 Cartridge 57 filtering 272/1000ml at 0.27ml/s with 24.1psi vacuum @23:30:49.46 Cartridge 57 filtering 286/1000ml at 0.21ml/s with 25.0psi vacuum @23:31:49.46 Cartridge 57 filtering 300/1000ml at 0.23ml/s with 22.8psi vacuum @23:32:49.46 Cartridge 57 filtering 313/1000ml at 0.21ml/s with 25.2psi vacuum @23:33:49.46 Cartridge 57 filtering 326/1000ml at 0.21ml/s with 24.2psi vacuum @23:34:49.46 Cartridge 57 filtering 338/1000ml at 0.20ml/s with 26.2psi vacuum @23:35:49.47 Cartridge 57 filtering 350/1000ml at 0.20ml/s with 25.0psi vacuum @23:36:49.46 Cartridge 57 filtering 362/1000ml at 0.20ml/s with 27.4psi vacuum @23:36:49.69 Flow averaged 0.30ml/s over 20:03 @23:36:49.72 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @23:36:50.55 Relieved excess vacuum in .9 seconds @23:36:50.57 SamplePump.seek 1000ml,53:38 @23:36:53.15 Flow estimate reduced to 0.24ml/s @23:36:53.18 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @23:36:54.00 Relieved excess vacuum in .9 seconds @23:36:54.03 SamplePump.seek 1000ml,53:36 @23:36:55.97 Flow estimate reduced to 0.19ml/s @23:36:55.99 Intake.close @23:36:56.03 Exhaust.close @23:36:56.26 Toroid.in :hold @23:36:56.32 Toroid.seek :bypass @23:36:57.95 Toroid.in :default @23:36:58.01 Toroid.seek :clear @23:36:59.61 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @23:36:59.62 Pressure.stop @23:36:59.78 Sampled 363.4ml @23:37:01.28 SPR.command "read_sequences" @23:37:01.88 SPR.command "stats_on" @23:37:02.00 SPR.command "start 0 1" @23:37:02.72 SPR.start /var/log/esp/spr23apr1323h.spr.bz2 @23:37:04.73 Handoff.select :waste @23:37:11.40 Syringe tare is 17% of full scale @23:37:11.42 PinPort.in :locking @23:37:11.47 PinPort.seek :locked @23:37:11.94 Archive.in :fast @23:37:11.99 Lysis2.in :fast @23:37:12.03 Lysis1.seek 1350uL @23:37:12.16 Archive.seek :full @23:37:12.21 Lysis2.seek :full @23:37:12.43 PinPort.setRawPosition! 2257 @23:37:12.47 Slide::Error in PROCESSING -- Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move @23:37:12.49 Waiting to Resume from Checkpoint "Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move" @23:41:38.10 Session Begun @23:41:43.38 -> showlog 80 @23:42:23.03 -> backtrace :PROCESSING @23:37:12 Slide::Error: Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move @23:43:33.10 -> PP Handoff Pin Port (PP) at cartridge - 163 counts @23:46:13.82 -> trouble >>> Thread[:PROCESSING] is suspended <<< @23:37:11 Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move @23:47:15.23 Ended log spr23apr1323h.spr.bz2 @23:47:15.25 SPR.command "stats_off" @23:48:16.36 -> Cmd.resume @23:48:16.39 Resuming Thread after Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move at 23:37:11 @23:48:16.41 Lysis1.seek 1350uL @23:48:16.52 Plunge.seek :reagent @23:48:17.41 Evacuating puck with filter bubble point of 50psi @23:48:17.43 PinPort.seek :locked @23:48:17.62 Handoff.select :waste @23:48:17.73 Handoff.select :blocked @23:48:17.91 PinPort.setRawPosition! 2262 @23:48:17.96 Slide::Error in PROCESSING -- Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 158 counts) while reading Air Syringe (AirS)'s status @23:48:17.98 Waiting to Resume from Checkpoint "Evacuating puck with filter bubble point of 50psi" @23:48:22.70 Cmd::Error in brent -- Not Processing @23:48:22.74 UserAbort in brent -- User Abort!! @23:48:27.86 -> PP.home.clear @23:48:27.88 PinPort.in :unlocking @23:48:27.97 PinPort.home.seek :clear Handoff Pin Port (PP) clear @23:49:02.16 -> Thread[:PROCESSING].exception [@23:37:12 Slide::Error: Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move, @23:48:17 Slide::Error: Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 158 counts) while reading Air Syringe (AirS)'s status] @23:49:10.12 -> Cmd.resume[0] @23:49:10.15 Resuming Thread (suspended since 23:48:17) @23:49:10.18 Evacuating puck with filter bubble point of 50psi @23:49:10.21 PinPort.in :locking @23:49:10.29 PinPort.seek :locked @23:49:10.77 Handoff.select :waste @23:49:11.88 Handoff.select :blocked @23:49:12.04 Cmd::Abort in PROCESSING -- aborted by brent @23:49:12.05 Waiting to Resume from Checkpoint "Evacuating puck with filter bubble point of 50psi" @23:49:45.89 UserAbort in brent -- User Abort!! @23:49:45.92 UserAbort in brent -- User Abort!! @23:49:45.97 -> Cmd.resume @23:49:46.01 Resuming Thread (suspended since 23:48:17) @23:49:46.04 Evacuating puck with filter bubble point of 50psi @23:49:46.08 PinPort.seek :locked @23:49:46.17 Handoff.select :waste @23:49:53.37 Air syringe pumping up to 1 strokes to reach 8psi @23:49:53.38 Air stroke #1 of 1 @23:49:53.87 Air.in :pressurize @23:49:53.94 Air.seek :pressurized @23:49:54.55 Air.in :default @23:49:54.65 Air.hold @23:49:55.35 Handoff.select :blocked @23:49:55.49 Cmd::Abort in PROCESSING -- aborted by brent during Air Syringe (AirS) move @23:49:55.50 Waiting to Resume from Checkpoint "aborted by brent during Air Syringe (AirS) move" @23:50:11.59 UserAbort in brent -- User Abort!! @23:50:11.62 UserAbort in brent -- User Abort!! @23:50:11.68 -> Cmd.resume[0] @23:50:11.71 Resuming Thread after aborted by brent during Air Syringe (AirS) move at 23:49:54 @23:50:11.76 Air.in :pressurize @23:50:11.85 Air.seek :pressurized @23:50:16.74 3.3psi after pressurize @23:50:16.75 Air.in :compress @23:50:16.83 Air.seek :evac @23:50:17.72 Reached 8.6 of 8.0psi after pumping 1472uL (0.92 strokes) @23:50:17.73 Air.in :default @23:50:17.82 Air.hold @23:50:17.94 Handoff.select :blocked @23:50:17.97 Overpressure in PROCESSING -- Waste port clogged (9.9psi) @23:50:18.00 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 259uL)" @23:50:37.04 Cmd::Error in brent -- Not Processing @23:50:37.10 UserAbort in brent -- User Abort!! @23:50:37.13 -> Cmd.resume @23:50:37.15 Resuming Thread after Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 259uL) at 23:50:16 @23:50:37.20 Air.in :compress @23:50:37.28 Air.seek :evac @23:50:37.46 Reached 24.4 of 8.0psi after pumping 2944uL (1.85 strokes) @23:50:37.47 Air.in :default @23:50:37.55 Air.hold @23:50:37.68 Handoff.select :blocked @23:50:37.71 Overpressure in PROCESSING -- Waste port clogged (24.4psi) @23:50:37.74 Waiting to Resume from Checkpoint "Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 258uL)" @23:50:57.05 Cmd::Error in brent -- Not Processing @23:50:57.09 UserAbort in brent -- User Abort!! @23:50:57.13 -> Cmd.thread @23:50:57.18 NoMethodError in brent -- private method `thread' called for Cmd:Module @23:50:57.21 -> trouble >>> Thread[:PROCESSING] is suspended <<< @23:37:11 Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move @23:48:17 Evacuating puck with filter bubble point of 50psi @23:49:53 Air syringe pumping up to 1 strokes to reach 8psi @23:49:54 aborted by brent during Air Syringe (AirS) move @23:50:16 Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 259uL) @23:50:37 Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 258uL) @23:51:04.24 -> trouble >>> Thread[:PROCESSING] is suspended <<< @23:37:11 Handoff Pin Port (PP) positionErr at cartridge (sensed cartridge - 162 counts) during Lysis Syringe #1 (LyS1) move @23:48:17 Evacuating puck with filter bubble point of 50psi @23:49:53 Air syringe pumping up to 1 strokes to reach 8psi @23:49:54 aborted by brent during Air Syringe (AirS) move @23:50:16 Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 259uL) @23:50:37 Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 258uL) @23:51:16.36 -> PP.home.clear @23:51:16.38 PinPort.in :unlocking @23:51:16.46 PinPort.home.seek :clear Handoff Pin Port (PP) clear @23:51:22.73 -> Cmd.resume[0] @23:51:22.75 Resuming Thread after Air Syringe (AirS) pressureOutOfBounds at 258uL (sensed 258uL) at 23:50:37 @23:51:22.79 Air.in :compress @23:51:22.87 Air.seek :evac @23:51:25.15 0.2psi after compress @23:51:35.72 0.1psi after compress (stable) @23:51:35.74 Air.in :default @23:51:35.82 Air.hold @23:51:36.00 at 0.1psi Opened to waste @23:51:36.01 Handoff.select :blocked @23:51:36.04 Archive syringe pumping up to 8 strokes to reach 50psi @23:51:36.06 Archive stroke #1 of 8 @23:51:36.17 Archive.in :pressurize @23:51:36.24 Archive.seek :pressurized @23:51:48.74 0.1psi after pressurize @23:51:54.31 0.1psi after pressurize (stable) @23:51:54.33 Archive.in :compress @23:51:54.43 Archive.seek :empty @23:51:59.94 Archive.in :default @23:52:00.04 Archive.hold @23:52:00.14 Handoff.select :blocked @23:52:00.17 Cmd::Abort in PROCESSING -- aborted by brent during Archive Syringe (ArS) move @23:52:00.19 Waiting to Resume from Checkpoint "aborted by brent during Archive Syringe (ArS) move" @23:54:26.30 UserAbort in brent -- User Abort!! @23:54:26.35 UserAbort in brent -- User Abort!! @23:54:26.40 -> list PinPort.method :connect def cartridge method_missing *Cartridge::Extend end def clear #always allow pin to be retracted to *Cartridge::Retract end alias_method :disconnect, :clear alias_method :connect, :cartridge end if defined? PinPort class Hash #this should go into standard lib someday def add! hash, &collisionHandler hash.each do |keys, value| keys.each{|key| update key=>value, &collisionHandler} end self end end @23:55:10.86 Session Begun @23:55:13.06 -> showlog @23:56:15.25 -> SPR.command "start 2 14" @23:56:15.27 SPR.command "start 2 14" @23:57:11.52 -> ESP.shutdown @23:57:11.68 Gate.power :heat,:OFF @23:57:11.70 Gate.power :cartridge,:OFF @23:57:11.74 Gate.power :motor,:OFF @23:57:11.77 Gate.power :core,:OFF @23:57:11.81 Gate.power :analytic1,:OFF @23:57:11.84 Gate.power :analytic2,:OFF @23:57:11.89 Gate.power :analytic3,:OFF @23:57:11.95 Gate.power :main,:OFF [:motor,:core,:analytic1,:analytic2,:analytic3,:main] @23:57:22.64 -> exit @23:57:22.66 Session Ended @23:59:43.03 -> exit @23:59:43.04 Session Ended @07:57:17.83PDT14-Apr-23 Session Begun @07:57:33.26 -> PP @07:57:33.30 BusOff in preston -- core bus was not powered while reading Handoff Pin Port (PP)'s status @07:58:04.58 -> exit @07:58:04.60 Session Ended @08:05:05.04 Session Begun @08:05:27.51 -> Cartridge.activate @08:05:27.54 BusOff in espclient -- core bus was not powered while reading Storage Carousel (SC)'s status @08:06:07.67 -> Power.status {analytic1: off with current limited to 5000mA, analytic2: off with current limited to 5000mA, analytic3: off with current limited to 5000mA, cartridge: off, core: off, heat: off with current limited to 5000mA, main: off with current limited to 5000mA, motor: off with current limited to 5000mA} @08:06:18.40 -> Power.core :on @08:06:18.42 Gate.power :core,:ON true @08:06:24.61 -> Power.status {analytic1: off with current limited to 5000mA, analytic2: off with current limited to 5000mA, analytic3: off with current limited to 5000mA, cartridge: off, core: ON, heat: off with current limited to 5000mA, main: off with current limited to 5000mA, motor: off with current limited to 5000mA} @08:06:36.21 -> Cartridge.activate @08:06:36.30 Slide::Error in espclient -- Storage Carousel (SC) Not yet Homed! @08:06:47.48 -> SC.home.jog 0 @08:06:47.51 BusOff in espclient -- Storage Carousel (SC) is not powered! @08:07:02.39 -> Handoff Handoff Valve::Manifold selects blocked @08:07:06.12 -> Handoff.waste @08:07:06.13 Handoff.select :waste @08:07:06.17 BusOff in espclient -- Handoff valve is not powered! @08:07:25.41 -> Power.main :on @08:07:25.42 Gate.power :main,:ON true @08:07:29.74 -> Cartridge.activate @08:07:29.83 Slide::Error in espclient -- Storage Carousel (SC) Not yet Homed! @08:07:36.35 -> Handoff.waste @08:07:36.36 Handoff.select :waste @08:07:36.38 BusOff in espclient -- Handoff valve is not powered! @08:07:42.53 -> Power.status {analytic1: off with current limited to 5000mA, analytic2: off with current limited to 5000mA, analytic3: off with current limited to 5000mA, cartridge: off, core: ON, heat: off with current limited to 5000mA, main: ON with current limited to 5000mA, motor: off with current limited to 5000mA} @08:07:56.97 -> Power.motor :on @08:07:56.98 Gate.power :motor,:ON true @08:08:00.18 -> Handoff.waste @08:08:00.19 Handoff.select :waste @08:08:00.32 Gate.power :analytic1,:ON,limit to 5000mA @08:08:00.34 Gate.power :analytic2,:ON,limit to 5000mA @08:08:00.37 Gate.power :analytic3,:ON,limit to 5000mA @08:08:01.08 Archive.configure :default @08:08:01.22 Air.configure :default @08:08:01.35 Lysis1.configure :default @08:08:01.48 Lysis2.configure :default @08:08:01.62 Plunge.configure :default @08:08:01.76 SamplePump.configure :default @08:08:01.91 Toroid.configure :default @08:08:02.06 Carousel.configure :locked @08:08:02.20 Pressure.configure :default @08:08:02.35 ExtraPump.configure :default @08:08:02.78 PinPort.configure :default @08:08:02.86 SPR Module 1, FW Rev. B, FPGA Rev. C @08:08:02.92 All elf controllers running firmware version 4.82 Handoff Valve::Manifold selects waste @08:08:09.58 -> Syringe.psi 0.00611930702793728 @08:08:19.12 -> SC @08:08:19.23 Slide::Error in espclient -- Storage Carousel (SC) Not yet Homed! @08:08:28.95 -> SC.home.jog 0 @08:08:28.97 Carousel.home.jog 0 Storage Carousel (SC) at 57 @08:09:06.91 -> PP.home.to :clear @08:09:06.93 PinPort.in :default @08:09:07.02 PinPort.home.seek :clear Handoff Pin Port (PP) clear @08:09:18.31 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2420 => ["cartridge",:end,:insert,:inserted,:locked]} @08:09:32.88 -> PP.jog 2420 @08:09:32.89 PinPort.jog 2420 Handoff Pin Port (PP) cartridge @08:09:51.75 -> PP.clear @08:09:51.77 PinPort.in :unlocking @08:09:51.85 PinPort.seek :clear Handoff Pin Port (PP) clear @08:10:02.81 -> PP.cartridge @08:10:02.82 Cartridge::TypeUnknown in espclient -- No cartridge is mounted @08:10:13.06 -> Cartridge.activate @08:10:13.17 Powering cartridge bus @08:10:15.78 Gate.setIR 1750,5000,0,6 @08:10:15.90 Gate.setIR 0,5000,0,6 @08:10:15.97 PuckHeater.configure :default @08:10:16.03 Gate.power :heat,:ON,limit to 5000mA @08:10:16.66 Mounted lysis Cartridge board#Q05080 @08:10:16.69 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:10:22.20 -> PP.cartridge @08:10:22.23 PinPort.in :locking @08:10:22.32 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:33.87 -> PP.clear @08:10:33.89 PinPort.in :unlocking @08:10:33.98 PinPort.seek :clear Handoff Pin Port (PP) clear @08:10:35.93 -> PP.cartridge @08:10:35.95 PinPort.in :locking @08:10:36.03 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:37.95 -> PP.cartridge @08:10:37.98 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:41.41 -> PP.clear @08:10:41.43 PinPort.in :unlocking @08:10:41.52 PinPort.seek :clear Handoff Pin Port (PP) clear @08:10:47.52 -> PP.cartridge @08:10:47.54 PinPort.in :locking @08:10:47.63 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:49.79 -> PP.clear @08:10:49.81 PinPort.in :unlocking @08:10:49.90 PinPort.seek :clear Handoff Pin Port (PP) clear @08:10:53.21 -> PP.cartridge @08:10:53.25 PinPort.in :locking @08:10:53.33 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:54.90 -> PP.clear @08:10:54.92 PinPort.in :unlocking @08:10:55.00 PinPort.seek :clear Handoff Pin Port (PP) clear @08:10:57.07 -> PP.cartridge @08:10:57.10 PinPort.in :locking @08:10:57.18 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:10:59.43 -> PP.clear @08:10:59.46 PinPort.in :unlocking @08:10:59.55 PinPort.seek :clear Handoff Pin Port (PP) clear @08:11:10.43 -> Cartridge.unlock @08:11:10.45 Gate.power :heat,:OFF @08:11:10.47 Gate.power :cartridge,:OFF @08:11:10.58 Carousel.in :default @08:11:10.70 Carousel.home.seek 57 @08:11:11.01 Slide::Error in espclient -- Archive Syringe (ArS) Not yet Homed! @08:11:17.75 -> ESP.ready! @08:11:17.87 Archive.in :default @08:11:18.27 Archive.home.jog 0 @08:11:18.50 Plunge.in :default @08:11:18.54 Lysis2.in :default @08:11:18.59 Toroid.in :default @08:11:18.63 Lysis1.in :default @08:11:18.69 Air.in :default @08:11:18.86 Plunge.home.jog 0 @08:11:18.92 Lysis2.home.jog 0 @08:11:18.97 Air.home.jog 0 @08:11:19.00 Toroid.home.jog 0 @08:11:19.07 Lysis1.home.jog 0 @08:11:19.47 Archive.in :homing @08:11:19.69 Archive.jog 8773 @08:11:19.73 Plunge.in :homing @08:11:19.76 Lysis2.in :homing @08:11:19.79 Lysis1.in :homing @08:11:19.85 Air.in :homing @08:11:19.90 Toroid.in :homing @08:11:20.10 Lysis2.jog 1336 @08:11:20.13 Plunge.jog 2267 @08:11:20.17 Lysis1.jog 3128 @08:11:20.20 Air.jog 9004 @08:11:20.25 Toroid.jog 634 @08:11:21.35 Lysis2.setRawPosition! 50 @08:11:21.41 Lysis2.seek :home @08:11:21.69 Lysis2.in :default @08:11:21.87 Plunge.setRawPosition! 50 @08:11:21.93 Plunge.seek :home @08:11:22.20 Plunge.in :default @08:11:22.32 Lysis1.setRawPosition! 50 @08:11:22.37 Lysis1.seek :home @08:11:22.53 Toroid.setRawPosition! 0 @08:11:22.58 Toroid.seek :home @08:11:22.68 Toroid.in :default @08:11:22.71 Lysis1.in :default @08:11:24.52 Archive.setRawPosition! 50 @08:11:24.58 Archive.seek :home @08:11:24.85 Archive.in :default @08:11:25.07 Air.setRawPosition! 50 @08:11:25.13 Air.seek :home @08:11:25.39 Air.in :default @08:11:25.72 Toroid.seek :clear @08:11:27.34 Carousel.in :default @08:11:27.46 Carousel.home.seek 57 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 57 Pressure = Absolute Pressure (Leak) Sensor @19psia PinPort = Handoff Pin Port (PP) clear @08:11:43.07 -> to 57 @08:11:43.33 Carousel.seek 57 @08:11:43.52 Lysis1.in :fast @08:11:43.59 Lysis1.seek :locked @08:11:46.19 Plunge.seek :locked @08:11:46.33 Air.in :fast @08:11:46.41 Air.seek :locked @08:11:47.98 Carousel.reconfigure :locked @08:11:48.06 Carousel.jog 0 @08:11:48.21 Powering cartridge bus @08:11:50.83 Gate.setIR 1750,5000,0,6 @08:11:50.95 Gate.setIR 0,5000,0,6 @08:11:51.02 PuckHeater.configure :default @08:11:51.09 Gate.power :heat,:ON,limit to 5000mA @08:11:51.72 Mounted lysis Cartridge board#Q05080 @08:11:51.76 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:11:55.33 -> PP.cartridge @08:11:55.35 PinPort.in :locking @08:11:55.44 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:12:03.54 -> PP.clear @08:12:03.56 PinPort.in :unlocking @08:12:03.65 PinPort.seek :clear Handoff Pin Port (PP) clear @08:12:21.27 -> to 59 @08:12:21.41 Gate.power :heat,:OFF @08:12:21.43 Gate.power :cartridge,:OFF @08:12:21.53 Carousel.in :default @08:12:21.66 Carousel.home.seek 57 @08:12:22.02 Air.in :unlocking @08:12:22.14 Air.seek :clear @08:12:23.50 Lysis1.in :unlocking @08:12:23.63 Lysis1.seek :clear @08:12:23.68 Plunge.in :unlocking @08:12:23.79 Plunge.seek :clear @08:12:25.03 Carousel.seek 59 @08:12:25.72 Lysis1.in :fast @08:12:25.80 Lysis1.seek :locked @08:12:28.39 Plunge.in :default @08:12:28.48 Plunge.seek :locked @08:12:28.59 Air.in :fast @08:12:28.66 Air.seek :locked @08:12:30.24 Carousel.reconfigure :locked @08:12:30.67 Carousel.jog 0 @08:12:30.90 Powering cartridge bus @08:12:33.51 Gate.setIR 1750,5000,0,6 @08:12:33.63 Gate.setIR 0,5000,0,6 @08:12:33.70 PuckHeater.configure :default @08:12:33.77 Gate.power :heat,:ON,limit to 5000mA @08:12:34.41 Mounted lysis Cartridge board#Q05058 @08:12:34.44 Using default cartridge heat loss curve for HeatingAccelInCarousel :lyseHold_phyto @08:12:39.31 -> PP.cartridge @08:12:39.34 PinPort.in :locking @08:12:39.42 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:12:47.49 -> Cartridge.unlock @08:12:47.51 Gate.power :heat,:OFF @08:12:47.54 Gate.power :cartridge,:OFF @08:12:47.63 PinPort.in :unlocking @08:12:47.71 PinPort.seek :clear @08:12:48.18 Carousel.in :default @08:12:48.30 Carousel.home.seek 59 @08:12:48.67 Air.in :unlocking @08:12:48.80 Air.seek :clear @08:12:50.14 Lysis1.in :unlocking @08:12:50.27 Lysis1.seek :clear @08:12:50.33 Plunge.in :unlocking @08:12:50.43 Plunge.seek :clear @08:12:55.77 -> to 2 @08:12:56.01 Carousel.seek 2 @08:13:07.91 Lysis1.in :fast @08:13:08.03 Lysis1.seek :locked @08:13:10.68 Plunge.in :default @08:13:10.74 Plunge.seek :locked @08:13:10.88 Air.in :fast @08:13:10.95 Air.seek :locked @08:13:12.52 Carousel.reconfigure :locked @08:13:12.60 Carousel.jog 0 @08:13:12.75 Powering cartridge bus @08:13:15.37 Gate.setIR 1750,5000,0,6 @08:13:15.50 Gate.setIR 0,5000,0,6 @08:13:15.56 PuckHeater.configure :default @08:13:15.63 Gate.power :heat,:ON,limit to 5000mA @08:13:16.26 Mounted lysis Cartridge board#Q05086 @08:13:16.29 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:13:59.68 -> PP.cartridge @08:13:59.70 PinPort.in :locking @08:13:59.79 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:14:06.81 -> Cartridge.unlock @08:14:06.83 Gate.power :heat,:OFF @08:14:06.85 Gate.power :cartridge,:OFF @08:14:06.96 PinPort.in :unlocking @08:14:07.04 PinPort.seek :clear @08:14:07.93 Carousel.in :default @08:14:08.05 Carousel.home.seek 2 @08:14:08.41 Air.in :unlocking @08:14:08.53 Air.seek :clear @08:14:09.89 Lysis1.in :unlocking @08:14:10.02 Lysis1.seek :clear @08:14:10.08 Plunge.in :unlocking @08:14:10.18 Plunge.seek :clear @08:14:37.30 -> to 52 @08:14:37.54 Carousel.seek 52 @08:14:48.02 Lysis1.in :fast @08:14:48.08 Lysis1.seek :locked @08:14:50.68 Plunge.in :default @08:14:50.77 Plunge.seek :locked @08:14:50.88 Air.in :fast @08:14:50.95 Air.seek :locked @08:14:52.53 Carousel.reconfigure :locked @08:14:52.60 Carousel.jog 0 @08:14:52.76 Powering cartridge bus @08:14:55.38 Gate.setIR 1750,5000,0,6 @08:14:55.51 Gate.setIR 0,5000,0,6 @08:14:55.58 PuckHeater.configure :default @08:14:55.64 Gate.power :heat,:ON,limit to 5000mA @08:14:56.30 Mounted lysis Cartridge board#Q05049 @08:14:56.34 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:15:10.08 -> PP.cartridge @08:15:10.10 PinPort.in :locking @08:15:10.19 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:15:16.04 -> Cartridge.unlock @08:15:16.06 Gate.power :heat,:OFF @08:15:16.08 Gate.power :cartridge,:OFF @08:15:16.19 PinPort.in :unlocking @08:15:16.26 PinPort.seek :clear @08:15:16.74 Carousel.in :default @08:15:16.86 Carousel.home.seek 52 @08:15:17.23 Air.in :unlocking @08:15:17.34 Air.seek :clear @08:15:18.66 Lysis1.in :unlocking @08:15:18.80 Lysis1.seek :clear @08:15:18.86 Plunge.in :unlocking @08:15:18.96 Plunge.seek :clear @08:15:27.41 -> SC Storage Carousel (SC) at 52 @08:15:34.46 -> to 30 @08:15:34.70 Carousel.seek 30 @08:15:39.46 Lysis1.in :fast @08:15:39.54 Lysis1.seek :locked @08:15:42.14 Plunge.in :default @08:15:42.26 Plunge.seek :locked @08:15:42.35 Air.in :fast @08:15:42.42 Air.seek :locked @08:15:44.00 Carousel.reconfigure :locked @08:15:44.08 Carousel.jog 0 @08:15:44.24 Powering cartridge bus @08:15:46.85 Gate.setIR 1750,5000,0,6 @08:15:46.97 Gate.setIR 0,5000,0,6 @08:15:47.04 Gate.power :cartridge,:OFF @08:15:47.57 Mounted archive Cartridge :archive_bac @08:15:47.66 -> to 31 @08:15:47.86 Carousel.in :default @08:15:47.97 Carousel.home.seek 30 @08:15:48.34 Air.in :unlocking @08:15:48.47 Air.seek :clear @08:15:49.85 Lysis1.in :unlocking @08:15:50.00 Lysis1.seek :clear @08:15:50.04 Plunge.in :unlocking @08:15:50.16 Plunge.seek :clear @08:15:51.40 Carousel.seek 31 @08:15:51.89 Lysis1.in :fast @08:15:51.96 Lysis1.seek :locked @08:15:54.57 Plunge.in :default @08:15:54.66 Plunge.seek :locked @08:15:54.77 Air.in :fast @08:15:54.85 Air.seek :locked @08:15:56.42 Carousel.reconfigure :locked @08:15:56.50 Carousel.jog 0 @08:15:56.65 Powering cartridge bus @08:15:59.27 Gate.setIR 1750,5000,0,6 @08:15:59.39 Gate.setIR 0,5000,0,6 @08:15:59.46 PuckHeater.configure :default @08:15:59.54 Gate.power :heat,:ON,limit to 5000mA @08:16:00.17 Mounted lysis Cartridge board#Q05052 @08:16:00.21 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:16:05.76 -> PP.cartridge @08:16:06.13 PinPort.in :locking @08:16:06.28 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:16:12.21 -> Cartridge.unlock @08:16:12.23 Gate.power :heat,:OFF @08:16:12.26 Gate.power :cartridge,:OFF @08:16:12.36 PinPort.in :unlocking @08:16:12.43 PinPort.seek :clear @08:16:12.91 Carousel.in :default @08:16:13.03 Carousel.home.seek 31 @08:16:13.39 Air.in :unlocking @08:16:13.52 Air.seek :clear @08:16:14.86 Lysis1.in :unlocking @08:16:15.00 Lysis1.seek :clear @08:16:15.06 Plunge.in :unlocking @08:16:15.15 Plunge.seek :clear @08:18:36.78 -> to 11 @08:18:37.04 Carousel.seek 11 @08:18:41.38 Lysis1.in :fast @08:18:41.46 Lysis1.seek :locked @08:18:44.07 Plunge.in :default @08:18:44.17 Plunge.seek :locked @08:18:44.26 Air.in :fast @08:18:44.34 Air.seek :locked @08:18:45.91 Carousel.reconfigure :locked @08:18:45.98 Carousel.jog 0 @08:18:46.13 Powering cartridge bus @08:18:48.75 Gate.setIR 1750,5000,0,6 @08:18:48.87 Gate.setIR 0,5000,0,6 @08:18:48.94 PuckHeater.configure :default @08:18:49.01 Gate.power :heat,:ON,limit to 5000mA @08:18:49.65 Mounted lysis Cartridge board#Q05041 @08:18:49.69 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:18:56.32 -> PP.cartridge @08:18:56.34 PinPort.in :locking @08:18:56.42 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:19:10.75 -> to 57 @08:19:10.90 Gate.power :heat,:OFF @08:19:10.92 Gate.power :cartridge,:OFF @08:19:11.02 PinPort.in :unlocking @08:19:11.08 PinPort.seek :clear @08:19:11.57 Carousel.in :default @08:19:11.69 Carousel.home.seek 11 @08:19:12.06 Air.in :unlocking @08:19:12.19 Air.seek :clear @08:19:13.97 Lysis1.in :unlocking @08:19:14.12 Lysis1.seek :clear @08:19:14.15 Plunge.in :unlocking @08:19:14.27 Plunge.seek :clear @08:19:15.50 Carousel.seek 57 @08:19:25.17 Lysis1.in :fast @08:19:25.28 Lysis1.seek :locked @08:19:27.93 Plunge.in :default @08:19:28.03 Plunge.seek :locked @08:19:28.12 Air.in :fast @08:19:28.20 Air.seek :locked @08:19:29.77 Carousel.reconfigure :locked @08:19:29.85 Carousel.jog 0 @08:19:30.01 Powering cartridge bus @08:19:32.63 Gate.setIR 1750,5000,0,6 @08:19:32.75 Gate.setIR 0,5000,0,6 @08:19:32.82 PuckHeater.configure :default @08:19:32.88 Gate.power :heat,:ON,limit to 5000mA @08:19:33.52 Mounted lysis Cartridge board#Q05080 @08:19:33.56 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:19:33.66 -> PP.cartridge @08:19:33.68 PinPort.in :locking @08:19:33.76 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:19:46.21 -> PP.clear @08:19:46.23 PinPort.in :unlocking @08:19:46.32 PinPort.seek :clear Handoff Pin Port (PP) clear @08:19:56.22 -> PhytoSampler.prepareLGcart @08:19:56.24 Handoff.select :waste @08:20:02.82 Syringe tare is 16% of full scale @08:20:02.84 PinPort.in :locking @08:20:02.93 PinPort.seek :locked @08:20:03.41 Archive.in :fast @08:20:03.47 Lysis2.in :fast @08:20:03.51 Lysis1.seek 1350uL @08:20:03.65 Lysis2.seek :full @08:20:03.68 Archive.seek :full Archive Syringe (ArS) full @08:31:32.69 -> ESP.reready! @08:31:33.14 Archive.in :default @08:31:33.25 Air.in :default @08:31:33.31 Lysis1.in :default @08:31:33.38 Archive.home.jog 0 @08:31:33.45 Lysis2.in :default @08:31:33.50 Plunge.in :default @08:31:33.57 Toroid.in :default @08:31:33.65 Carousel.in :default @08:31:33.68 PinPort.in :default @08:31:33.85 Air.home.jog 0 @08:31:33.89 Lysis1.home.jog 0 @08:31:34.05 Toroid.home.jog 0 @08:31:34.07 Plunge.home.jog 0 @08:31:34.14 Lysis2.home.jog 0 @08:31:34.28 Carousel.home.jog 0 @08:31:34.37 PinPort.home.jog 0 @08:31:34.90 Archive.in :homing @08:31:35.18 Archive.jog 1292 @08:31:35.23 Toroid.in :homing @08:31:35.26 Plunge.in :homing @08:31:35.31 Lysis2.in :homing @08:31:35.34 Lysis1.in :homing @08:31:35.40 Air.in :homing @08:31:35.44 PinPort.in :homing @08:31:35.67 Toroid.jog 630 @08:31:35.73 Plunge.jog 1531 @08:31:35.77 Lysis2.jog 1329 @08:31:35.81 Lysis1.jog 3132 @08:31:35.84 PinPort.jog -2420 @08:31:35.89 Air.jog 1987 @08:31:36.60 Archive.setRawPosition! 50 @08:31:36.66 Archive.seek :home @08:31:36.93 Archive.in :default @08:31:37.08 Lysis2.setRawPosition! 50 @08:31:37.14 Lysis2.seek :home @08:31:37.22 Plunge.setRawPosition! 50 @08:31:37.28 Plunge.seek :home @08:31:37.41 Lysis2.in :default @08:31:37.51 Air.setRawPosition! 50 @08:31:37.57 Air.seek :home @08:31:37.64 Plunge.in :default @08:31:37.86 Air.in :default @08:31:38.01 Lysis1.setRawPosition! 50 @08:31:38.03 Toroid.setRawPosition! 0 @08:31:38.12 Lysis1.seek :home @08:31:38.16 Toroid.seek :home @08:31:38.31 Toroid.in :default @08:31:38.48 Lysis1.in :default @08:31:39.42 PinPort.setRawPosition! 0 @08:31:39.48 PinPort.seek :home @08:31:39.56 PinPort.in :default @08:31:39.89 Toroid.seek :clear @08:31:41.50 Carousel.in :default @08:31:41.62 Carousel.home.seek 57 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 57 Pressure = Absolute Pressure (Leak) Sensor @19psia PinPort = Handoff Pin Port (PP) clear PuckHeater = Puck Heater at 22.6C @08:31:43.88 -> to 21 @08:31:44.18 Carousel.seek 21 @08:31:51.68 Gate.power :heat,:OFF @08:31:51.69 Gate.power :cartridge,:OFF @08:31:51.84 Lysis1.in :fast @08:31:51.91 Lysis1.seek :locked @08:31:54.52 Plunge.seek :locked @08:31:54.66 Air.in :fast @08:31:54.73 Air.seek :locked @08:31:56.31 Carousel.reconfigure :locked @08:31:56.39 Carousel.jog 0 @08:31:56.54 Powering cartridge bus @08:31:59.16 Gate.setIR 1750,5000,0,6 @08:31:59.28 Gate.setIR 0,5000,0,6 @08:31:59.35 PuckHeater.configure :default @08:31:59.42 Gate.power :heat,:ON,limit to 5000mA @08:32:00.06 Mounted lysis Cartridge board#Q05038 @08:32:00.11 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:32:00.19 -> PP.cartridge @08:32:00.22 PinPort.in :locking @08:32:00.30 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:33:03.11 -> Cmd.stop @08:33:03.22 Handoff.select :close @08:33:03.36 Gate.power :heat,:OFF @08:33:03.38 Gate.power :cartridge,:OFF @08:33:03.48 PinPort.in :unlocking @08:33:03.55 PinPort.seek :clear @08:33:04.01 Carousel.in :default @08:33:04.14 Carousel.home.seek 21 @08:33:04.45 Plunge.seek :locked @08:33:04.65 Air.in :unlocking @08:33:04.78 Air.seek :clear @08:33:04.86 Lysis1.in :unlocking @08:33:04.99 Lysis1.seek :clear @08:33:06.46 Gate.power :motor,:OFF @08:33:06.48 Gate.power :core,:OFF @08:33:06.50 Gate.power :analytic1,:OFF @08:33:06.54 Gate.power :analytic2,:OFF @08:33:06.56 Gate.power :analytic3,:OFF @08:33:06.59 Gate.power :main,:OFF @08:33:06.61 Safely stopped and ready to power off :STOPPED @08:33:09.90 -> ESP.ready! @08:33:10.31 Gate.power :main,:ON,limit to 5000mA @08:33:10.32 Gate.power :analytic1,:ON,limit to 5000mA @08:33:10.35 Gate.power :analytic2,:ON,limit to 5000mA @08:33:10.38 Gate.power :analytic3,:ON,limit to 5000mA @08:33:10.46 Powering core bus @08:33:12.81 SPR Module 1, FW Rev. B, FPGA Rev. C @08:33:13.63 Archive.configure :default @08:33:13.73 Air.configure :default @08:33:13.80 Lysis1.configure :default @08:33:13.87 Lysis2.configure :default @08:33:13.97 Plunge.configure :default @08:33:14.06 SamplePump.configure :default @08:33:14.16 Toroid.configure :default @08:33:14.25 Carousel.configure :default @08:33:14.35 Pressure.configure :default @08:33:14.44 ExtraPump.configure :default @08:33:14.51 PinPort.configure :default @08:33:14.59 All elf controllers running firmware version 4.83 @08:33:14.61 Gate.power :motor,:ON,limit to 5000mA @08:33:14.80 Archive.home.jog 0 @08:33:15.32 Toroid.home.jog 0 @08:33:15.37 Plunge.home.jog 0 @08:33:15.41 Air.home.jog 0 @08:33:15.44 PinPort.home.jog 0 @08:33:15.47 Lysis1.home.jog 0 @08:33:15.51 Carousel.home.jog 0 @08:33:15.54 Lysis2.home.jog 0 @08:33:16.24 Archive.in :homing @08:33:16.45 Archive.jog 1385 @08:33:16.49 Toroid.in :homing @08:33:16.53 Plunge.in :homing @08:33:16.56 Lysis2.in :homing @08:33:16.62 Lysis1.in :homing @08:33:16.65 Air.in :homing @08:33:16.69 PinPort.in :homing @08:33:16.96 Toroid.jog 630 @08:33:17.00 Plunge.jog 1525 @08:33:17.03 Lysis2.jog 1386 @08:33:17.07 Lysis1.jog 1401 @08:33:17.09 Air.jog 1382 @08:33:17.14 PinPort.jog -108 @08:33:17.55 PinPort.setRawPosition! 0 @08:33:17.61 PinPort.seek :home @08:33:17.69 PinPort.in :default @08:33:17.91 Archive.setRawPosition! 50 @08:33:17.97 Archive.seek :home @08:33:18.25 Archive.in :default @08:33:18.37 Lysis2.setRawPosition! 50 @08:33:18.43 Lysis2.seek :home @08:33:18.53 Plunge.setRawPosition! 50 @08:33:18.56 Lysis1.setRawPosition! 50 @08:33:18.59 Air.setRawPosition! 50 @08:33:18.71 Lysis1.seek :home @08:33:18.75 Plunge.seek :home @08:33:18.79 Air.seek :home @08:33:18.96 Lysis2.in :default @08:33:19.09 Lysis1.in :default @08:33:19.20 Plunge.in :default @08:33:19.22 Air.in :default @08:33:19.36 Toroid.setRawPosition! 0 @08:33:19.42 Toroid.seek :home @08:33:19.51 Toroid.in :default @08:33:20.02 Toroid.seek :clear @08:33:21.64 Carousel.in :default @08:33:21.77 Carousel.home.seek 21 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 21 Pressure = Absolute Pressure (Leak) Sensor @19psia PinPort = Handoff Pin Port (PP) clear @08:33:28.93 -> to 60 @08:33:29.17 Carousel.seek 60 @08:33:37.40 Lysis1.in :fast @08:33:37.48 Lysis1.seek :locked @08:33:40.08 Plunge.seek :locked @08:33:40.21 Air.in :fast @08:33:40.28 Air.seek :locked @08:33:41.86 Carousel.reconfigure :locked @08:33:42.00 Powering cartridge bus @08:33:44.61 Gate.setIR 1750,5000,0,6 @08:33:44.73 Gate.setIR 0,5000,0,6 @08:33:44.80 PuckHeater.configure :default @08:33:44.86 Gate.power :heat,:ON,limit to 5000mA @08:33:45.50 Mounted lysis Cartridge board#Q05062 @08:33:45.54 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:33:56.58 -> PP.cartridge @08:33:56.61 PinPort.in :locking @08:33:56.70 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:34:03.69 -> Cmd.stop @08:34:03.79 Gate.power :heat,:OFF @08:34:03.81 Gate.power :cartridge,:OFF @08:34:03.92 PinPort.in :unlocking @08:34:03.99 PinPort.seek :clear @08:34:04.47 Carousel.in :default @08:34:04.59 Carousel.home.seek 60 @08:34:04.89 Plunge.seek :locked @08:34:05.09 Air.in :unlocking @08:34:05.22 Air.seek :clear @08:34:05.30 Lysis1.in :unlocking @08:34:05.44 Lysis1.seek :clear @08:34:06.90 Gate.power :motor,:OFF @08:34:06.92 Gate.power :core,:OFF @08:34:06.95 Gate.power :analytic1,:OFF @08:34:06.97 Gate.power :analytic2,:OFF @08:34:07.00 Gate.power :analytic3,:OFF @08:34:07.03 Gate.power :main,:OFF @08:34:07.05 Safely stopped and ready to power off :STOPPED @08:34:14.98 -> to 4 @08:34:15.44 BusOff in espclient -- core bus was not powered while reading Plunge Valve (PV)'s status @08:34:26.09 -> ESP.ready!; to 4 @08:34:26.12 Gate.power :main,:ON,limit to 5000mA @08:34:26.15 Gate.power :analytic1,:ON,limit to 5000mA @08:34:26.17 Gate.power :analytic2,:ON,limit to 5000mA @08:34:26.21 Gate.power :analytic3,:ON,limit to 5000mA @08:34:26.29 Powering core bus @08:34:28.63 SPR Module 1, FW Rev. B, FPGA Rev. C @08:34:28.97 Archive.configure :default @08:34:29.07 Air.configure :default @08:34:29.14 Lysis1.configure :default @08:34:29.21 Lysis2.configure :default @08:34:29.31 Plunge.configure :default @08:34:29.40 SamplePump.configure :default @08:34:29.49 Toroid.configure :default @08:34:29.59 Carousel.configure :default @08:34:29.68 Pressure.configure :default @08:34:29.77 ExtraPump.configure :default @08:34:29.85 PinPort.configure :default @08:34:29.91 All elf controllers running firmware version 4.83 @08:34:29.93 Gate.power :motor,:ON,limit to 5000mA @08:34:30.24 Archive.home.jog 0 @08:34:30.39 Air.home.jog 0 @08:34:30.50 Lysis1.home.jog 0 @08:34:30.61 Lysis2.home.jog 0 @08:34:30.76 Plunge.home.jog 0 @08:34:31.06 Carousel.home.jog 0 @08:34:31.12 Toroid.home.jog 0 @08:34:31.21 PinPort.home.jog 0 @08:34:31.70 Plunge.in :homing @08:34:31.75 Lysis1.in :homing @08:34:31.78 Air.in :homing @08:34:31.82 Archive.in :homing @08:34:31.87 Lysis2.in :homing @08:34:32.05 Plunge.jog 1534 @08:34:32.09 Lysis1.jog 1397 @08:34:32.14 Air.jog 1385 @08:34:32.17 Lysis2.jog 1386 @08:34:32.23 Archive.jog 1382 @08:34:32.51 PinPort.in :homing @08:34:32.54 Toroid.in :homing @08:34:32.64 Toroid.jog 620 @08:34:32.67 PinPort.jog -107 @08:34:32.95 PinPort.setRawPosition! 0 @08:34:33.00 PinPort.seek :home @08:34:33.09 PinPort.in :default @08:34:33.43 Plunge.setRawPosition! 50 @08:34:33.51 Plunge.seek :home @08:34:33.61 Lysis2.setRawPosition! 50 @08:34:33.64 Lysis1.setRawPosition! 50 @08:34:33.67 Air.setRawPosition! 50 @08:34:33.70 Archive.setRawPosition! 50 @08:34:33.86 Lysis2.seek :home @08:34:33.91 Lysis1.seek :home @08:34:33.94 Air.seek :home @08:34:34.02 Archive.seek :home @08:34:34.19 Plunge.in :default @08:34:34.28 Lysis2.in :default @08:34:34.83 Air.in :default @08:34:34.86 Lysis1.in :default @08:34:35.00 Archive.in :default @08:34:35.02 Toroid.setRawPosition! 0 @08:34:35.12 Toroid.seek :home @08:34:35.22 Toroid.in :default @08:34:35.39 Toroid.seek :clear @08:34:37.00 Carousel.in :default @08:34:37.12 Carousel.home.seek 60 @08:34:37.58 Carousel.seek 4 @08:34:49.27 Lysis1.in :fast @08:34:49.34 Lysis1.seek :locked @08:34:51.95 Plunge.seek :locked @08:34:52.08 Air.in :fast @08:34:52.16 Air.seek :locked @08:34:53.73 Carousel.reconfigure :locked @08:34:53.88 Powering cartridge bus @08:34:56.49 Gate.setIR 1750,5000,0,6 @08:34:56.61 Gate.setIR 0,5000,0,6 @08:34:56.68 PuckHeater.configure :default @08:34:56.75 Gate.power :heat,:ON,limit to 5000mA @08:34:57.38 Mounted lysis Cartridge board#Q05076 @08:34:57.42 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:36:07.59 -> Cmd.stop @08:36:07.68 Gate.power :heat,:OFF @08:36:07.70 Gate.power :cartridge,:OFF @08:36:07.79 Carousel.in :default @08:36:07.92 Carousel.home.seek 4 @08:36:08.23 Plunge.seek :locked @08:36:08.43 Air.in :unlocking @08:36:08.59 Air.seek :clear @08:36:08.63 Lysis1.in :unlocking @08:36:08.79 Lysis1.seek :clear @08:36:10.26 Gate.power :motor,:OFF @08:36:10.28 Gate.power :core,:OFF @08:36:10.30 Gate.power :analytic1,:OFF @08:36:10.33 Gate.power :analytic2,:OFF @08:36:10.35 Gate.power :analytic3,:OFF @08:36:10.38 Gate.power :main,:OFF @08:36:10.41 Safely stopped and ready to power off :STOPPED @08:36:17.16 -> ESP.ready!; to 43 @08:36:17.21 Gate.power :main,:ON,limit to 5000mA @08:36:17.22 Gate.power :analytic1,:ON,limit to 5000mA @08:36:17.25 Gate.power :analytic2,:ON,limit to 5000mA @08:36:17.27 Gate.power :analytic3,:ON,limit to 5000mA @08:36:17.35 Powering core bus @08:36:19.70 SPR Module 1, FW Rev. B, FPGA Rev. C @08:36:20.04 Archive.configure :default @08:36:20.14 Air.configure :default @08:36:20.21 Lysis1.configure :default @08:36:20.27 Lysis2.configure :default @08:36:20.36 Plunge.configure :default @08:36:20.94 SamplePump.configure :default @08:36:21.03 Toroid.configure :default @08:36:21.13 Carousel.configure :default @08:36:21.23 Pressure.configure :default @08:36:21.32 ExtraPump.configure :default @08:36:21.38 PinPort.configure :default @08:36:21.46 All elf controllers running firmware version 4.83 @08:36:21.48 Gate.power :motor,:ON,limit to 5000mA @08:36:21.88 Archive.home.jog 0 @08:36:21.98 Air.home.jog 0 @08:36:22.09 Lysis1.home.jog 0 @08:36:22.21 Lysis2.home.jog 0 @08:36:22.29 Plunge.home.jog 0 @08:36:22.35 Toroid.home.jog 0 @08:36:22.50 Carousel.home.jog 0 @08:36:22.55 PinPort.home.jog 0 @08:36:23.23 Air.in :homing @08:36:23.26 Archive.in :homing @08:36:23.33 Lysis1.in :homing @08:36:23.47 Air.jog 1385 @08:36:23.52 Archive.jog 1384 @08:36:23.55 Lysis1.jog 1397 @08:36:23.79 PinPort.in :homing @08:36:23.82 Toroid.in :homing @08:36:23.86 Plunge.in :homing @08:36:23.90 Lysis2.in :homing @08:36:24.07 PinPort.jog -107 @08:36:24.10 Toroid.jog 626 @08:36:24.13 Plunge.jog 1524 @08:36:24.16 Lysis2.jog 1386 @08:36:24.42 PinPort.setRawPosition! 0 @08:36:24.47 PinPort.seek :home @08:36:24.56 PinPort.in :default @08:36:24.73 Archive.setRawPosition! 50 @08:36:24.81 Air.setRawPosition! 50 @08:36:24.83 Archive.seek :home @08:36:24.96 Air.seek :home @08:36:25.04 Lysis1.setRawPosition! 50 @08:36:25.09 Lysis1.seek :home @08:36:25.20 Archive.in :default @08:36:25.29 Air.in :default @08:36:25.39 Lysis1.in :default @08:36:25.47 Lysis2.setRawPosition! 50 @08:36:25.54 Lysis2.seek :home @08:36:25.63 Plunge.setRawPosition! 50 @08:36:25.69 Plunge.seek :home @08:36:25.82 Lysis2.in :default @08:36:25.97 Plunge.in :default @08:36:26.37 Toroid.setRawPosition! 0 @08:36:26.43 Toroid.seek :home @08:36:26.51 Toroid.in :default @08:36:26.57 Toroid.seek :clear @08:36:28.19 Carousel.in :default @08:36:28.31 Carousel.home.seek 4 @08:36:29.20 Carousel.seek 43 @08:36:37.43 Lysis1.in :fast @08:36:37.50 Lysis1.seek :locked @08:36:40.11 Plunge.seek :locked @08:36:40.25 Air.in :fast @08:36:40.32 Air.seek :locked @08:36:41.90 Carousel.reconfigure :locked @08:36:42.04 Powering cartridge bus @08:36:44.65 Gate.setIR 1750,5000,0,6 @08:36:44.78 Gate.setIR 0,5000,0,6 @08:36:44.84 PuckHeater.configure :default @08:36:44.90 Gate.power :heat,:ON,limit to 5000mA @08:36:45.54 Mounted lysis Cartridge board#Q05053 @08:36:45.58 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:36:47.05 -> PP.cartridge @08:36:47.07 PinPort.in :locking @08:36:47.15 PinPort.seek :locked Handoff Pin Port (PP) cartridge @08:36:58.81 -> Cartridge.unlock @08:36:58.83 Gate.power :heat,:OFF @08:36:58.85 Gate.power :cartridge,:OFF @08:36:58.96 PinPort.in :unlocking @08:36:59.03 PinPort.seek :clear @08:36:59.51 Carousel.in :default @08:36:59.63 Carousel.home.seek 43 @08:37:00.00 Air.in :unlocking @08:37:00.11 Air.seek :clear @08:37:01.44 Lysis1.in :unlocking @08:37:01.57 Lysis1.seek :clear @08:37:01.60 Plunge.in :unlocking @08:37:01.72 Plunge.seek :clear @08:37:24.83 -> Cartridge.unlock @08:37:24.92 Carousel.in :default @08:37:25.03 Carousel.home.seek 43 @08:37:31.33 -> SC.to 57 @08:37:31.35 Carousel.seek 57 Storage Carousel (SC) at 57 @08:49:41.17 -> Cartridge.dry [] @08:49:43.61 -> slots {[56,52..48,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, [44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,5,3] => { Type: :archive_bac, State: :reserved, was: :dry}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 57 => { Type: :daSPR_phyto, State: :lysing, filtered: 363.4, spr: spr23apr1323h.spr.bz2}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :lysing, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @08:49:55.91 -> slot 57=:dry @08:49:55.93 SyntaxError in espclient -- compile error espclient:78: syntax error, unexpected '=', expecting $end slot 57=:dry ^ @08:50:02.78 -> slot 57=>:dry {57 => { Type: :daSPR_phyto, State: :dry, filtered: 363.4, spr: spr23apr1323h.spr.bz2}} @08:50:12.72 -> slot 57=>nil {57 => nil} @08:50:36.11 -> slot 57=>[:dry,:daSPR_phyto] {57 => { Type: :daSPR_phyto, State: :dry}} @08:50:40.49 -> Cartridge.dry [57] @08:54:04.48 -> Cartridge.dry [57] @08:54:19.08 -> to 57 @08:54:19.33 Carousel.seek 57 @08:54:19.53 Lysis1.in :fast @08:54:19.60 Lysis1.seek :locked @08:54:22.21 Plunge.in :default @08:54:22.27 Plunge.seek :locked @08:54:22.42 Air.in :fast @08:54:22.49 Air.seek :locked @08:54:24.06 Carousel.reconfigure :locked @08:54:24.21 Powering cartridge bus @08:54:26.83 Gate.setIR 1750,5000,0,6 @08:54:26.95 Gate.setIR 0,5000,0,6 @08:54:27.02 PuckHeater.configure :default @08:54:27.08 Gate.power :heat,:ON,limit to 5000mA @08:54:27.72 Mounted lysis Cartridge board#Q05080 @08:54:27.76 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @08:55:13.43 -> PhytoSampler.prepareLGcart @08:55:13.45 Handoff.select :waste @08:55:20.14 Syringe tare is 17% of full scale @08:55:20.16 PinPort.in :locking @08:55:20.25 PinPort.seek :locked @08:55:20.73 Archive.in :fast @08:55:20.79 Lysis2.in :fast @08:55:20.82 Lysis1.seek 1350uL @08:55:20.97 Archive.seek :full @08:55:21.00 Lysis2.seek :full Archive Syringe (ArS) full @08:55:27.63 -> Cmd.stop @08:55:27.74 Handoff.select :close @08:55:27.88 Gate.power :heat,:OFF @08:55:27.90 Gate.power :cartridge,:OFF @08:55:28.00 PinPort.in :unlocking @08:55:28.07 PinPort.seek :clear @08:55:28.55 Carousel.in :default @08:55:28.67 Carousel.home.seek 57 @08:55:28.97 Plunge.seek :locked @08:55:29.54 Archive.in :unlocking @08:55:29.68 Archive.seek :clear @08:55:29.76 Air.in :unlocking @08:55:29.89 Air.seek :clear @08:55:29.95 Lysis1.in :unlocking @08:55:30.13 Lysis2.in :unlocking @08:55:30.16 Lysis1.seek :clear @08:55:30.28 Lysis2.seek :clear @08:55:32.04 Gate.power :motor,:OFF @08:55:32.06 Gate.power :core,:OFF @08:55:32.08 Gate.power :analytic1,:OFF @08:55:32.11 Gate.power :analytic2,:OFF @08:55:32.13 Gate.power :analytic3,:OFF @08:55:32.17 Gate.power :main,:OFF @08:55:32.19 Safely stopped and ready to power off :STOPPED @08:55:37.96 -> MainThread.exit # @08:55:38.04 ThreadError in espclient -- killed thread @08:55:38.10 Session Ended nohup: procesAll: No such file or directory @08:57:10.58PDT14-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @08:57:10.60 >BREAK< ESP3 Gateway v1.42 for cartridge bus @08:57:13.11 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @08:57:13.58 Sea pressure (Abs) tare is 6% of full scale @08:57:17.75 Polling Can every 2:00 @08:57:17.79 Loading /home/esp/esp2/bin/processAll mission @08:57:17.96 Sea pressure (Abs) tare is 6% of full scale @08:57:17.97 Sampling default goal volumes @08:57:17.98 Cartridge group: dry @08:57:18.03 Gate.power :main,:ON,limit to 5000mA @08:57:18.04 Gate.power :analytic1,:ON,limit to 5000mA @08:57:18.05 Gate.power :analytic2,:ON,limit to 5000mA @08:57:18.06 Gate.power :analytic3,:ON,limit to 5000mA @08:57:18.17 Powering core bus @08:57:20.47 SPR Module 1, FW Rev. B, FPGA Rev. C @08:57:20.87
Archive.configure :default @08:57:20.94 Air.configure :default @08:57:20.99 Lysis1.configure :default @08:57:21.04 Lysis2.configure :default @08:57:21.12 Plunge.configure :default @08:57:21.54 SamplePump.configure :default @08:57:21.62 Toroid.configure :default @08:57:21.69 Carousel.configure :default @08:57:21.76 Pressure.configure :default @08:57:21.84 ExtraPump.configure :default @08:57:21.89 PinPort.configure :default @08:57:21.94 All elf controllers running firmware version 4.83 @08:57:21.96 Gate.power :motor,:ON,limit to 5000mA @08:57:22.36 Archive.home.jog 0 @08:57:22.40 Air.home.jog 0 @08:57:22.46 Lysis1.home.jog 0 @08:57:22.55 Lysis2.home.jog 0 @08:57:22.64 Plunge.home.jog 0 @08:57:22.71 Toroid.home.jog 0 @08:57:22.81 Carousel.home.jog 0 @08:57:22.92 PinPort.home.jog 0 @08:57:23.32 Archive.in :homing @08:57:23.51 Toroid.in :homing @08:57:23.57 Plunge.in :homing @08:57:23.60 Lysis2.in :homing @08:57:23.63 Lysis1.in :homing @08:57:23.66 Air.in :homing @08:57:23.70 Archive.jog 1386 @08:57:23.90 Plunge.jog 1531 @08:57:23.94 Toroid.jog 634 @08:57:23.96 Lysis1.jog 1398 @08:57:23.98 Lysis2.jog 1387 @08:57:24.00 Air.jog 1385 @08:57:24.23 PinPort.in :homing @08:57:24.29 PinPort.jog -107 @08:57:24.50 PinPort.setRawPosition! 0 @08:57:24.54 PinPort.seek :home @08:57:24.59 PinPort.in :default @08:57:25.03 Archive.setRawPosition! 50 @08:57:25.08 Archive.seek :home @08:57:25.25 Plunge.setRawPosition! 50 @08:57:25.28 Lysis1.setRawPosition! 50 @08:57:25.34 Plunge.seek :home @08:57:25.39 Lysis1.seek :home @08:57:25.52 Air.setRawPosition! 50 @08:57:25.55 Lysis2.setRawPosition! 50 @08:57:25.57 Archive.in :default @08:57:25.64 Air.seek :home @08:57:25.71 Lysis2.seek :home @08:57:25.84 Plunge.in :default @08:57:25.89 Lysis1.in :default @08:57:26.02 Lysis2.in :default @08:57:26.04 Air.in :default @08:57:26.17 Toroid.setRawPosition! 0 @08:57:26.21 Toroid.seek :home @08:57:26.28 Toroid.in :default @08:57:26.39
Toroid.seek :clear @08:57:28.00 Carousel.in :default @08:57:28.10 Carousel.home.seek 57 @08:57:28.33 Selecting Cartridge 57 @08:57:28.57 Carousel.seek 57 @08:57:29.12 Lysis1.in :fast @08:57:29.17 Lysis1.seek :locked @08:57:31.75 Plunge.seek :locked @08:57:31.88 Air.in :fast @08:57:31.93 Air.seek :locked @08:57:33.50
Carousel.reconfigure :locked @08:57:33.63 Powering cartridge bus @08:57:36.24 Gate.setIR 1750,5000,0,6 @08:57:36.35 Gate.setIR 0,5000,0,6 @08:57:36.41 PuckHeater.configure :default @08:57:36.45 Gate.power :heat,:ON,limit to 5000mA @08:57:39.13 Mounted lysis Cartridge board#Q05080 @08:57:39.15 Using default cartridge heat loss curve for HeatingAccelInCarousel @08:57:39.41 Preparing to filter cartridge 57 @08:57:39.65 Priming sample loop w/100ml, bypass w/10ml @08:57:39.67 SamplePump.setPosition! 0ml @08:57:39.70 Bypass.open @08:57:41.17 Intake.open @08:57:41.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:57:44.49 Vacuum tare is 60% of full scale @08:57:44.60 Pressure.in :default @08:57:44.65 Pressure.hold @08:57:44.82 SamplePump.in :prime @08:57:44.88 SamplePump.seek 10ml,59 seconds @08:57:53.60 Pressure.stop @08:57:53.64 SamplePump.setPosition! 0ml @08:57:53.68 Exhaust.open @08:57:55.12 Bypass.close @08:57:56.60 Pressure.in :default @08:57:56.65 Pressure.hold @08:57:56.87 SamplePump.in :prime @08:57:56.93 SamplePump.seek 100ml,5:16 @08:59:21.89 Pressure.stop @08:59:21.95 Bypass.open @08:59:22.64 Intake.close @08:59:23.70 Toroid.in :hold @08:59:23.82 Toroid.seek :crack @08:59:25.38 Toroid.seek :cartridge @08:59:26.49 Toroid.in :default @08:59:26.54 Toroid.seek :clear @08:59:28.50 Pressurizing cartridge to 26psi above 15.0psia @08:59:28.51 SamplePump.setPosition! 0ml @08:59:28.55 Intake.open @08:59:29.21 Bypass.close @08:59:29.90 Exhaust.close @08:59:30.69 Pressure.in :default @08:59:30.74 Pressure.hold @08:59:30.91 SamplePump.in :charge @08:59:30.97 SamplePump.seek 2.3ml,42 seconds @08:59:45.01 Pressure.stop @08:59:45.05 Intake.close @08:59:45.77 Pressurized cartridge with 0.941ml to 26.1psi @08:59:45.79 Isolated @39.0psia (24.0psi) @08:59:45.80 Verifying cartridge holds at least 0.65..0.85 of 39.0psia @08:59:45.81 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @08:59:50.41 Cartridge pressure fell 5% to 36.9psia @08:59:50.48 Sampling 1000.0ml in 1:25:20 @08:59:50.50 SamplePump.setPosition! 0ml @08:59:50.54 Exhaust.open @08:59:51.26 Intake.open @08:59:52.07 Pressure.in :default @08:59:52.12 Pressure.hold @08:59:52.23 Sea 2.2m deep (17.9psia) @08:59:52.24 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @08:59:52.36 SamplePump.in :sample @08:59:52.42 SamplePump.seek 1000ml,1:23:51 @08:59:55.11 Cartridge 57 filtering 1/1000ml at 0.45ml/s with 24.9psi vacuum @09:00:55.12 Cartridge 57 filtering 30/1000ml at 0.47ml/s with 25.4psi vacuum @09:01:55.12 Cartridge 57 filtering 57/1000ml at 0.44ml/s with 24.4psi vacuum @09:02:55.12 Cartridge 57 filtering 84/1000ml at 0.43ml/s with 19.8psi vacuum @09:03:55.12 Cartridge 57 filtering 109/1000ml at 0.43ml/s with 22.3psi vacuum 1.8m deep (17.3psia) @09:04:55.12 Cartridge 57 filtering 134/1000ml at 0.42ml/s with 24.9psi vacuum @09:05:55.12 Cartridge 57 filtering 159/1000ml at 0.40ml/s with 22.9psi vacuum 2.5m deep (18.4psia) @09:06:55.12 Cartridge 57 filtering 183/1000ml at 0.40ml/s with 25.3psi vacuum @09:07:55.19 Cartridge 57 filtering 207/1000ml at 0.40ml/s with 24.2psi vacuum @09:08:55.12 Cartridge 57 filtering 229/1000ml at 0.37ml/s with 25.5psi vacuum 2.3m deep (18.1psia) @09:09:55.12 Cartridge 57 filtering 251/1000ml at 0.37ml/s with 25.7psi vacuum @09:10:55.12 Cartridge 57 filtering 273/1000ml at 0.34ml/s with 24.8psi vacuum @09:11:55.12 Cartridge 57 filtering 293/1000ml at 0.35ml/s with 22.9psi vacuum 3.1m deep (19.3psia) @09:12:55.12 Cartridge 57 filtering 313/1000ml at 0.31ml/s with 26.2psi vacuum 1.9m deep (17.4psia) @09:13:55.12 Cartridge 57 filtering 332/1000ml at 0.30ml/s with 24.3psi vacuum 1.9m deep (17.5psia) @09:14:55.12 Cartridge 57 filtering 351/1000ml at 0.30ml/s with 24.1psi vacuum 2.1m deep (17.8psia) @09:15:55.11 Cartridge 57 filtering 368/1000ml at 0.27ml/s with 23.0psi vacuum 1.8m deep (17.4psia) @09:16:55.12 Cartridge 57 filtering 385/1000ml at 0.27ml/s with 25.9psi vacuum 2.4m deep (18.2psia) @09:17:55.12 Cartridge 57 filtering 402/1000ml at 0.30ml/s with 24.9psi vacuum 2.5m deep (18.4psia) @09:18:55.11 Cartridge 57 filtering 418/1000ml at 0.27ml/s with 21.2psi vacuum 2.6m deep (18.5psia) @09:19:55.12 Cartridge 57 filtering 433/1000ml at 0.24ml/s with 22.3psi vacuum 2.8m deep (18.9psia) @09:20:55.12 Cartridge 57 filtering 448/1000ml at 0.22ml/s with 22.1psi vacuum 2.8m deep (18.9psia) @09:21:55.12 Cartridge 57 filtering 462/1000ml at 0.23ml/s with 24.0psi vacuum @09:22:55.12 Cartridge 57 filtering 476/1000ml at 0.22ml/s with 24.3psi vacuum @09:23:55.12 Cartridge 57 filtering 489/1000ml at 0.24ml/s with 24.1psi vacuum @09:24:55.12 Cartridge 57 filtering 501/1000ml at 0.21ml/s with 22.8psi vacuum @09:25:55.12 Cartridge 57 filtering 514/1000ml at 0.20ml/s with 22.6psi vacuum @09:26:55.12 Cartridge 57 filtering 526/1000ml at 0.20ml/s with 24.4psi vacuum 2.0m deep (17.6psia) @09:27:55.12 Cartridge 57 filtering 538/1000ml at 0.20ml/s with 24.5psi vacuum 1.9m deep (17.4psia) @09:28:55.12 Cartridge 57 filtering 551/1000ml at 0.21ml/s with 24.2psi vacuum 3.0m deep (19.1psia) @09:29:55.12 Cartridge 57 filtering 563/1000ml at 0.20ml/s with 25.9psi vacuum 2.3m deep (18.1psia) @09:30:55.12 Cartridge 57 filtering 575/1000ml at 0.20ml/s with 25.5psi vacuum 2.9m deep (19.0psia) @09:31:55.12 Cartridge 57 filtering 588/1000ml at 0.20ml/s with 26.8psi vacuum 2.6m deep (18.6psia) @09:32:53.84 Flow averaged 0.30ml/s over 33:02 @09:32:53.87 Waiting up to 9 seconds for vacuum to reduce from 26.4 to 18.0psi @09:32:54.70 Relieved excess vacuum in .9 seconds @09:32:54.72 SamplePump.seek 1000ml,33:52 @09:32:55.11 Cartridge 57 filtering 600/1000ml at 0.13ml/s with 3.8psi vacuum 3.0m deep (19.1psia) @09:33:13.88 Flow averaged 0.20ml/s over 20 seconds @09:33:13.91 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @09:33:14.74 Relieved excess vacuum in .9 seconds @09:33:14.76 SamplePump.seek 1000ml,33:32 @09:33:19.50 Flow estimate reduced to 0.16ml/s @09:33:19.52 Intake.close @09:33:19.55 Exhaust.close @09:33:20.20 Toroid.in :hold @09:33:20.25 Toroid.seek :bypass @09:33:21.88 Toroid.in :default @09:33:21.94 Toroid.seek :clear @09:33:23.54 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @09:33:23.55 Pressure.stop @09:33:23.71 Sampled 604.6ml @09:33:25.23 SPR.command "read_sequences" @09:33:25.37 SPR.command "stats_on" @09:33:25.50 SPR.command "start 0 1" @09:33:26.22 SPR.start /var/log/esp/spr23apr1409h.spr.bz2 @09:33:28.22 Handoff.select :waste @09:33:34.90 Syringe tare is 16% of full scale @09:33:34.92 PinPort.in :locking @09:33:34.97 PinPort.seek :locked @09:33:35.44 Archive.in :fast @09:33:35.48 Lysis2.in :fast @09:33:35.54 Lysis1.seek 1350uL @09:33:35.64 Archive.seek :full @09:33:35.70 Lysis2.seek :full @09:33:37.67 Plunge.seek :reagent @09:33:38.54 Evacuating puck with filter bubble point of 50psi @09:33:38.55 PinPort.seek :locked @09:33:38.62 Handoff.select :waste @09:33:45.43 Air syringe pumping up to 1 strokes to reach 8psi @09:33:45.44 Air stroke #1 of 1 @09:33:45.52 Air.in :pressurize @09:33:45.57 Air.seek :pressurized @09:33:51.28 0.4psi after pressurize @09:33:51.30 Air.in :compress @09:33:51.35 Air.seek :evac @09:33:54.34 0.8psi after compress @09:34:04.92 0.1psi after compress (stable) @09:34:04.93 Air.in :default @09:34:04.98 Air.hold @09:34:05.15 at 0.1psi Opened to waste @09:34:05.16 Handoff.select :blocked @09:34:05.27 Archive syringe pumping up to 8 strokes to reach 50psi @09:34:05.28 Archive stroke #1 of 8 @09:34:05.35 Archive.in :pressurize @09:34:05.40 Archive.seek :pressurized @09:34:17.89 9.1psi after pressurize @09:34:42.00 23.4psi after pressurize (stable) @09:34:42.01 Archive.in :compress @09:34:42.07 Archive.seek :empty @09:34:56.67 33.9psi after compress @09:35:08.27 36.8psi after compress (stable) @09:35:08.29 Archive.in :gulp @09:35:08.33 Archive.seek :topVent @09:35:14.56 36.9psi after gulp @09:35:20.13 36.8psi after gulp (stable) @09:35:20.14 Archive stroke #2 of 8 @09:35:20.22 Archive.in :pressurize @09:35:20.27 Archive.seek :pressurized @09:35:32.75 36.2psi after pressurize @09:35:38.34 36.2psi after pressurize (stable) @09:35:38.37 Archive.in :compress @09:35:38.43 Archive.seek :empty @09:35:51.28 Reached 50.2 of 50.0psi after pumping 3422uL (1.98 strokes) @09:35:51.29 Archive.in :default @09:35:51.34 Archive.hold @09:35:51.40 Handoff.select :waste @09:35:58.57 delay 1:00 @09:36:58.59 Handoff.select :blocked @09:37:21.26 Handoff.select :waste @09:37:21.38 2.7psi at evacEnd @09:37:21.40 Handoff.select :blocked @09:37:21.52 Archive.in :fast @09:37:21.57 Archive.seek :topVent @09:37:35.09 Stable @2.9psi after Handoff blocked @09:37:35.10 Archive syringe pumping up to 8 strokes to reach 33psi @09:37:35.11 Archive stroke #1 of 8 @09:37:35.19 Archive.in :pressurize @09:37:35.24 Archive.seek :pressurized @09:37:47.71 3.8psi after pressurize @09:37:47.73 Archive.in :compress @09:37:47.78 Archive.seek :empty @09:38:02.38 6.5psi after compress @09:38:37.96 16.7psi after compress (stable) @09:38:37.99 Archive.in :gulp @09:38:38.07 Archive.seek :topVent @09:38:44.30 17.8psi after gulp @09:38:49.88 18.3psi after gulp (stable) @09:38:49.89 Archive stroke #2 of 8 @09:38:49.95 Archive.in :pressurize @09:38:50.01 Archive.seek :pressurized @09:39:02.49 19.2psi after pressurize @09:39:02.50 Archive.in :compress @09:39:02.55 Archive.seek :empty @09:39:17.21 22.0psi after compress @09:39:46.79 29.7psi after compress (stable) @09:39:46.81 Archive.in :gulp @09:39:46.85 Archive.seek :topVent @09:39:53.08 30.6psi after gulp @09:39:58.65 30.9psi after gulp (stable) @09:39:58.66 Archive stroke #3 of 8 @09:39:58.73 Archive.in :pressurize @09:39:58.78 Archive.seek :pressurized @09:40:11.26 31.2psi after pressurize @09:40:11.27 Archive.in :compress @09:40:11.32 Archive.seek :empty @09:40:25.36 Reached 33.0 of 32.9psi after pumping 5177uL (2.99 strokes) @09:40:25.38 Archive.in :default @09:40:25.42 Archive.hold @09:40:25.48 Handoff.select :waste @09:40:32.65 delay 1:00 @09:41:32.66 Handoff.select :blocked @09:41:55.33 Handoff.select :waste @09:41:55.44 1.9psi at evacEnd @09:41:55.46 Air.seek :fullVent @09:41:56.86 Archive.in :fast @09:41:56.91 Archive.seek :topVent @09:42:03.95 Seawater evacuated, Pressure above/below filter released. @09:42:04.02 Lysis1.in :slow @09:42:04.07 Lysis1.seek 1050uL @09:42:34.09 delay 5 seconds @09:42:39.11 Handoff.select :blocked @09:42:39.28 Lysis1.seek 450uL @09:43:39.25 delay 3 seconds @09:43:42.32 Air.in mix with 1.40048 parts Lysis1 @09:43:42.81 Air.seek :evac @09:43:42.86 Lysis1.seek 300uL @09:43:57.95 Lysis1.seek :empty @09:44:28.04 Lysis buffer delivered @14.7psi @09:44:28.05 Polling Can every 2:00 @09:44:28.59 PuckHeater.configure :default #ambient 22.7C @09:44:28.62 PuckHeater.seek 79.7C @09:45:16.46 PuckHeater.hold 2:00,79.7C @09:47:16.16 Polling Can every 2:00 @09:47:16.70 PuckHeater.hold 30 seconds,78.7C @09:47:46.79 Polling Can every 2:00 @09:47:47.33 PuckHeater.configure :default #ambient 22.7C @09:47:47.37 PuckHeater.hold 30 seconds,78.1C @09:48:17.47 Polling Can every 2:00 @09:48:18.01 PuckHeater.configure :default #ambient 22.7C @09:48:18.05 PuckHeater.hold 10:00,77.6C @09:58:16.45 Lysis complete. Syringe pressure @17.8psi @09:58:16.47 Archive syringe pumping up to 5 strokes to reach 40psi @09:58:16.49 Archive stroke #1 of 5 @09:58:16.58 Archive.in :pressurize @09:58:16.64 Archive.seek :pressurized @09:58:29.12 17.4psi after pressurize @09:58:54.19 32.2psi after pressurize (stable) @09:58:54.21 Archive.in :compress @09:58:54.26 Archive.seek :empty @09:59:01.29 Reached 40.3 of 40.0psi after pumping 1566uL (0.91 strokes) @09:59:01.30 Archive.in :default @09:59:01.35 Archive.hold @09:59:01.40 delay 2 seconds @09:59:03.43 Archive.in :fast @09:59:03.47 Archive.seek :full @09:59:10.05 Lysis1.seek 138uL @09:59:23.92 delay 1 second @09:59:24.94 Archive.seek :full @09:59:24.98 Lysis1.seek :empty @09:59:38.89 Handoff.select :blocked @09:59:38.96 Initial air pressure @46.5psi @09:59:39.39 Lysis1.in :extract @09:59:39.49 Lysis1.seek 600uL,nil @09:59:41.20 Archive syringe pumping up to 6 strokes to reach 25psi @09:59:41.21 Archive stroke #1 of 6 @09:59:41.28 Archive.in :pressurize @09:59:41.34 Archive.seek :pressurized @09:59:53.85 15.9psi after pressurize @10:01:00.65 Archive.in :default @10:01:00.71 Archive.hold @10:01:00.83 Initial 600uL lysate @16.1psi @10:01:00.84 Handoff.select :blocked @10:01:00.92 Initial air pressure @16.2psi @10:01:01.02 Lysis1.seek 825uL,nil @10:01:01.74 Archive syringe pumping up to 6 strokes to reach 17psi @10:01:01.75 Archive stroke #1 of 6 @10:01:01.81 Archive.in :compress @10:01:01.87 Archive.seek :empty @10:01:16.50 16.3psi after compress @10:01:16.74 Reached 17.3 of 17.0psi after pumping 1730uL (1.00 strokes) @10:01:16.76 Archive.in :default @10:01:16.82 Archive.hold @10:01:17.53 Archive syringe pumping up to 6 strokes to reach 17psi @10:01:17.54 Archive stroke #1 of 6 @10:01:17.66 Archive.in :compress @10:01:17.71 Archive.seek :empty @10:01:17.87 13.1psi after compress @10:01:36.94 15.0psi after compress (stable) @10:01:42.12 Archive.in :gulp @10:01:42.18 Archive.seek :topVent @10:01:48.43 15.4psi after gulp @10:01:54.00 15.5psi after gulp (stable) @10:01:54.06 Archive stroke #2 of 6 @10:01:54.18 Archive.in :pressurize @10:01:54.23 Archive.seek :pressurized @10:02:06.71 15.3psi after pressurize @10:02:12.33 15.5psi after pressurize (stable) @10:02:12.34 Archive.in :compress @10:02:12.39 Archive.seek :empty @10:02:26.99 15.3psi after compress @10:02:29.79 Archive.in :default @10:02:29.85 Archive.hold @10:02:30.50 Recovered 825uL lysate @16.1psi @10:02:30.51 Air.in :halfSpeed @10:02:30.57 Air.seek :fullVent @10:02:32.77 Puck Top Vented, bottom @16.0psi @10:02:32.78 Handoff.select :waste @10:02:32.90 delay 5 seconds @10:02:37.97 Handoff open to waste, bottom @0.3psi @10:02:37.98 Air.in :default @10:02:38.03 Air.seek :evac @10:02:39.45 Lysis2.in :DAmix @10:02:39.58 Lysis1.in :DAmix @10:02:39.98 Lysis2.seek 1480uL @10:02:40.02 Lysis1.seek 575uL @10:03:15.09 delay 5 seconds @10:03:20.10 Handoff.select SPR @10:03:20.25 SPR.command "start 2 14" @10:03:21.52 SPR stabilized @10:03:21.64 delay 10 seconds @10:03:31.65 SPR.command "start 1 0" @10:03:34.88 Waiting up to 4:00 for SPR to enter state 1 @10:05:39.16 SPR.command "hold_on" @10:05:40.55 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:05:41.06 Lysis2.seek 1180uL @10:05:41.11 Lysis1.seek 275uL @10:06:23.12 delay 15 seconds @10:06:38.14 SPR.command "hold_off" @10:06:41.20 Waiting up to 38:00 for SPR to enter state 7 @10:24:40.82 SPRsummary:64.9RIU,52.5RIU,39.8RIU,80.6RIU,high,high,high after 3248s @10:30:41.70 Session Begun @10:30:46.90 -> slot {[56,52..48,43,41,39,37,35,33,31,29,27,25,23,21,19,17,15,13,11,9,7,4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, [44,42,40,38,36,34,32,30,28,26,24,22,20,18,16,14,12,10,8,6,5,3] => { Type: :archive_bac, State: :reserved, was: :dry}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 57 => { Type: :daSPR_phyto, State: :analyzing, filtered: 604.6, lysed: 825, spr: spr23apr1409h.spr.bz2}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :lysing, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @10:31:18.04 SPR analysis completed @10:31:25.20 Ended log spr23apr1409h.spr.bz2 @10:31:25.22 SPR.command "stats_off" @10:31:26.19 Handoff.select :close @10:31:26.33
Gate.power :heat,:OFF @10:31:26.34 Gate.power :cartridge,:OFF @10:31:26.43 PinPort.in :unlocking @10:31:26.51 PinPort.seek :clear @10:31:26.99 Carousel.in :default @10:31:27.10 Carousel.home.seek 57 @10:31:27.40 Plunge.seek :locked @10:31:28.27 Archive.in :unlocking @10:31:28.40 Archive.seek :clear @10:31:28.44 Air.in :unlocking @10:31:28.62 Air.seek :clear @10:31:28.67 Lysis1.in :unlocking @10:31:28.84 Lysis1.seek :clear @10:31:28.89 Lysis2.in :unlocking @10:31:29.02 Lysis2.seek :clear @10:31:33.00
Gate.power :motor,:OFF @10:31:33.02 Gate.power :core,:OFF @10:31:33.04 Gate.power :analytic1,:OFF @10:31:33.07 Gate.power :analytic2,:OFF @10:31:33.09 Gate.power :analytic3,:OFF @10:31:33.12 Gate.power :main,:OFF @10:31:33.15 Safely stopped and ready to power off @10:31:33.58 Cartridge::None in MAIN -- Not enough dry cartridges remain @10:31:33.63 Session Ended @13:17:05.74PDT14-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:17:05.76 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:17:08.20 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @13:17:08.66 Sea pressure (Abs) tare is 6% of full scale @13:17:12.70 Polling Can every 2:00 @13:17:12.73 Loading /home/esp/esp2/bin/processAll mission @13:17:12.90 Sea pressure (Abs) tare is 6% of full scale @13:17:12.91 Sampling maximum of 250.0ml per cartridge @13:17:12.92 Cartridge group: dry @13:17:13.05 Gate.power :analytic1,:ON,limit to 5000mA @13:17:13.06 Gate.power :analytic2,:ON,limit to 5000mA @13:17:13.07 Gate.power :analytic3,:ON,limit to 5000mA @13:17:13.65 Archive.configure :default @13:17:13.77 Air.configure :default @13:17:13.82 Air.hold @13:17:14.20 Air.configure :default @13:17:14.30 SPR Module 1, FW Rev. B, FPGA Rev. C @13:17:14.37
Lysis1.configure :default @13:17:14.42 Lysis1.hold @13:17:14.47 Lysis1.configure :default @13:17:14.58 Lysis2.configure :default @13:17:14.70 Plunge.configure :default @13:17:14.75 Plunge.hold @13:17:14.80 Plunge.configure :default @13:17:14.92 SamplePump.configure :default @13:17:15.04 Toroid.configure :default @13:17:15.09 Toroid.hold @13:17:15.14 Toroid.configure :default @13:17:15.26 Carousel.configure :default @13:17:15.31 Carousel.hold @13:17:15.36 Carousel.configure :default @13:17:15.49 Pressure.configure :default @13:17:15.61 ExtraPump.configure :default @13:17:15.71 PinPort.configure :default @13:17:15.76 PinPort.hold @13:17:15.81 PinPort.configure :default @13:17:15.86 All elf controllers running firmware version 4.83 @13:17:16.42 Toroid.seek :clear @13:17:16.48 Carousel.in :default @13:17:16.59 Carousel.home.seek 1 @13:17:16.91 Carousel.reconfigure :locked @13:17:17.08 Gate.setIR 1750,5000,0,6 @13:17:17.20 Gate.setIR 0,5000,0,6 @13:17:17.25 PuckHeater.configure :default @13:17:17.29 Gate.power :heat,:ON,limit to 5000mA @13:17:17.99 Mounted lysis Cartridge board#Q05034 @13:17:18.02 Using this cartridge's heat loss curve for HeatingAccelInCarousel @13:17:18.05 Selecting Cartridge 56 @13:17:18.17 Gate.power :heat,:OFF @13:17:18.18 Gate.power :cartridge,:OFF @13:17:18.25 PinPort.in :unlocking @13:17:18.30 PinPort.seek :clear @13:17:18.75 Carousel.in :default @13:17:18.85 Carousel.home.seek 1 @13:17:19.19 Air.in :unlocking @13:17:19.33 Air.seek :clear @13:17:20.67 Lysis1.in :unlocking @13:17:21.14 Lysis1.seek :clear @13:17:21.21 Plunge.in :unlocking @13:17:21.27 Plunge.seek :clear @13:17:22.46
Carousel.seek 56 @13:17:33.94 Lysis1.in :fast @13:17:33.98 Lysis1.seek :locked @13:17:36.56 Plunge.in :default @13:17:36.64 Plunge.seek :locked @13:17:36.73 Air.in :fast @13:17:36.78 Air.seek :locked @13:17:38.33
Carousel.reconfigure :locked @13:17:38.46 Powering cartridge bus @13:17:41.07 Gate.setIR 1750,5000,0,6 @13:17:41.19 Gate.setIR 0,5000,0,6 @13:17:41.24 PuckHeater.configure :default @13:17:41.28 Gate.power :heat,:ON,limit to 5000mA @13:17:41.91 Mounted lysis Cartridge board#Q05060 @13:17:41.93 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:17:42.17 Preparing to filter cartridge 56 @13:17:42.40 Priming sample loop w/100ml, bypass w/10ml @13:17:42.41 SamplePump.setPosition! 0ml @13:17:42.44 Exhaust.close @13:17:42.50 Bypass.open @13:17:43.94 Intake.open @13:17:44.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:17:47.22 Vacuum tare is 59% of full scale @13:17:47.34 Pressure.in :default @13:17:47.39 Pressure.hold @13:17:47.56 SamplePump.in :prime @13:17:47.62 SamplePump.seek 10ml,59 seconds @13:17:56.33 Pressure.stop @13:17:56.36 SamplePump.setPosition! 0ml @13:17:56.40 Exhaust.open @13:17:57.82 Bypass.close @13:17:59.26 Pressure.in :default @13:17:59.31 Pressure.hold @13:17:59.90 SamplePump.in :prime @13:17:59.96 SamplePump.seek 100ml,5:16 @13:19:29.28 Pressure.stop @13:19:29.34 Bypass.open @13:19:29.98 Intake.close @13:19:30.43 Toroid.in :hold @13:19:30.48 Toroid.seek :crack @13:19:32.00 Toroid.seek :cartridge @13:19:33.09 Toroid.in :default @13:19:33.14 Toroid.seek :clear @13:19:35.08 Pressurizing cartridge to 26psi above 14.7psia @13:19:35.10 SamplePump.setPosition! 0ml @13:19:35.13 Intake.open @13:19:35.80 Bypass.close @13:19:36.46 Exhaust.close @13:19:37.27 Pressure.in :default @13:19:37.32 Pressure.hold @13:19:37.48 SamplePump.in :charge @13:19:37.54 SamplePump.seek 2.3ml,42 seconds @13:19:54.09 Pressure.stop @13:19:54.14 Intake.close @13:19:54.86 Pressurized cartridge with 1.311ml to 26.2psi @13:19:54.87 Isolated @36.8psia (22.2psi) @13:19:54.88 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @13:19:54.90 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:19:59.48 Cartridge pressure fell 3% to 35.7psia @13:19:59.55 Sampling 250.0ml in 1:25:20 @13:19:59.56 SamplePump.setPosition! 0ml @13:19:59.60 Exhaust.open @13:20:00.33 Intake.open @13:20:01.12 Pressure.in :default @13:20:01.17 Pressure.hold @13:20:01.27 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @13:20:01.39 SamplePump.in :sample @13:20:01.45 SamplePump.seek 250ml,21:21 @13:20:04.17 Cartridge 56 filtering 1/250ml at 0.55ml/s with 21.1psi vacuum @13:21:04.18 Cartridge 56 filtering 30/250ml at 0.47ml/s with 24.9psi vacuum @13:22:04.17 Cartridge 56 filtering 59/250ml at 0.49ml/s with 23.5psi vacuum @13:23:04.18 Cartridge 56 filtering 87/250ml at 0.46ml/s with 25.8psi vacuum @13:24:04.57 Cartridge 56 filtering 114/250ml at 0.45ml/s with 23.5psi vacuum @13:25:04.18 Cartridge 56 filtering 139/250ml at 0.38ml/s with 22.8psi vacuum @13:26:04.18 Cartridge 56 filtering 161/250ml at 0.36ml/s with 24.4psi vacuum @13:27:04.18 Cartridge 56 filtering 183/250ml at 0.34ml/s with 25.9psi vacuum @13:28:04.18 Cartridge 56 filtering 203/250ml at 0.35ml/s with 25.0psi vacuum @13:29:04.18 Cartridge 56 filtering 223/250ml at 0.31ml/s with 24.0psi vacuum @13:30:04.18 Cartridge 56 filtering 241/250ml at 0.28ml/s with 23.9psi vacuum @13:30:33.50 Pressure.stop @13:30:33.60 Intake.close @13:30:33.64 Exhaust.close @13:30:33.81 Toroid.in :hold @13:30:33.86 Toroid.seek :bypass @13:30:35.48 Toroid.in :default @13:30:35.53 Toroid.seek :clear @13:30:37.13 Sampled 250.0ml @13:30:38.62 SPR.command "read_sequences" @13:30:39.12 SPR.command "stats_on" @13:30:39.24 SPR.command "start 0 1" @13:30:39.97 SPR.start /var/log/esp/spr23apr1413h.spr.bz2 @13:30:41.97 Handoff.select :waste @13:30:48.64 Syringe tare is 16% of full scale @13:30:48.66 PinPort.in :locking @13:30:48.71 PinPort.seek :locked @13:30:49.17 Archive.in :fast @13:30:49.22 Lysis2.in :fast @13:30:49.26 Lysis1.seek 1350uL @13:30:49.38 Archive.seek :full @13:30:49.43 Lysis2.seek :full @13:30:51.36 Plunge.seek :reagent @13:30:52.64 Evacuating puck with filter bubble point of 50psi @13:30:52.66 PinPort.seek :locked @13:30:52.72 Handoff.select :waste @13:30:59.30 Air syringe pumping up to 1 strokes to reach 8psi @13:30:59.31 Air stroke #1 of 1 @13:30:59.38 Air.in :pressurize @13:30:59.43 Air.seek :pressurized @13:31:05.16 1.4psi after pressurize @13:31:05.19 Air.in :compress @13:31:05.25 Air.seek :evac @13:31:08.23 0.6psi after compress @13:31:18.81 -0.1psi after compress (stable) @13:31:18.82 Air.in :default @13:31:18.87 Air.hold @13:31:19.43 at -0.1psi Opened to waste @13:31:19.44 Handoff.select :blocked @13:31:19.56 Archive syringe pumping up to 8 strokes to reach 50psi @13:31:19.57 Archive stroke #1 of 8 @13:31:19.63 Archive.in :pressurize @13:31:19.68 Archive.seek :pressurized @13:31:32.15 0.1psi after pressurize @13:31:37.73 0.1psi after pressurize (stable) @13:31:37.75 Archive.in :compress @13:31:37.79 Archive.seek :empty @13:31:52.40 9.2psi after compress @13:32:02.97 9.6psi after compress (stable) @13:32:02.99 Archive.in :gulp @13:32:03.04 Archive.seek :topVent @13:32:09.27 10.2psi after gulp @13:32:14.84 10.4psi after gulp (stable) @13:32:14.85 Archive stroke #2 of 8 @13:32:14.92 Archive.in :pressurize @13:32:14.97 Archive.seek :pressurized @13:32:27.46 10.9psi after pressurize @13:32:33.04 11.2psi after pressurize (stable) @13:32:33.06 Archive.in :compress @13:32:33.11 Archive.seek :empty @13:32:48.11 16.3psi after compress @13:33:25.21 35.4psi after compress (stable) @13:33:25.23 Archive.in :gulp @13:33:25.27 Archive.seek :topVent @13:33:31.50 36.5psi after gulp @13:33:37.07 37.1psi after gulp (stable) @13:33:37.08 Archive stroke #3 of 8 @13:33:37.47 Archive.in :pressurize @13:33:37.61 Archive.seek :pressurized @13:33:50.09 37.8psi after pressurize @13:33:55.66 38.2psi after pressurize (stable) @13:33:55.68 Archive.in :compress @13:33:55.72 Archive.seek :empty @13:34:10.33 40.8psi after compress @13:34:29.82 Reached 50.0 of 50.0psi after pumping 5190uL (3.00 strokes) @13:34:29.84 Archive.in :default @13:34:29.88 Archive.hold @13:34:29.94 Handoff.select :waste @13:34:44.11 delay 1:00 @13:35:44.12 Handoff.select :blocked @13:36:06.81 Handoff.select :waste @13:36:06.92 1.3psi at evacEnd @13:36:06.93 Handoff.select :blocked @13:36:07.04 Archive.in :fast @13:36:07.09 Archive.seek :topVent @13:36:20.75 Stable @1.0psi after Handoff blocked @13:36:20.76 Archive syringe pumping up to 8 strokes to reach 31psi @13:36:20.77 Archive stroke #1 of 8 @13:36:20.84 Archive.in :pressurize @13:36:20.89 Archive.seek :pressurized @13:36:33.37 1.3psi after pressurize @13:36:33.40 Archive.in :compress @13:36:33.45 Archive.seek :empty @13:36:48.05 2.6psi after compress @13:37:06.13 5.2psi after compress (stable) @13:37:06.15 Archive.in :gulp @13:37:06.20 Archive.seek :topVent @13:37:12.42 6.2psi after gulp @13:37:17.99 6.8psi after gulp (stable) @13:37:18.00 Archive stroke #2 of 8 @13:37:18.07 Archive.in :pressurize @13:37:18.12 Archive.seek :pressurized @13:37:30.61 8.0psi after pressurize @13:37:30.63 Archive.in :compress @13:37:30.67 Archive.seek :empty @13:37:45.31 11.9psi after compress @13:38:29.39 24.1psi after compress (stable) @13:38:29.41 Archive.in :gulp @13:38:29.45 Archive.seek :topVent @13:38:35.68 25.1psi after gulp @13:38:41.26 25.6psi after gulp (stable) @13:38:41.27 Archive stroke #3 of 8 @13:38:41.34 Archive.in :pressurize @13:38:41.39 Archive.seek :pressurized @13:38:53.87 26.5psi after pressurize @13:38:53.89 Archive.in :compress @13:38:53.94 Archive.seek :empty @13:39:08.57 29.2psi after compress @13:39:12.96 Reached 31.0 of 31.0psi after pumping 5190uL (3.00 strokes) @13:39:12.98 Archive.in :default @13:39:13.02 Archive.hold @13:39:13.08 Handoff.select :waste @13:39:22.25 delay 1:00 @13:40:22.27 Handoff.select :blocked @13:40:44.95 Handoff.select :waste @13:40:45.08 1.0psi at evacEnd @13:40:45.10 Air.seek :fullVent @13:40:46.50 Archive.in :fast @13:40:46.55 Archive.seek :topVent @13:40:53.64 Seawater evacuated, Pressure above/below filter released. @13:40:53.70 Lysis1.in :slow @13:40:53.75 Lysis1.seek 1050uL @13:41:23.83 delay 5 seconds @13:41:28.85 Handoff.select :blocked @13:41:29.02 Lysis1.seek 450uL @13:42:28.96 delay 3 seconds @13:42:32.05 Air.in mix with 1.40048 parts Lysis1 @13:42:32.54 Air.seek :evac @13:42:32.59 Lysis1.seek 300uL @13:42:47.67 Lysis1.seek :empty @13:43:17.75 Lysis buffer delivered @13.4psi @13:43:17.76 Polling Can every 2:00 @13:43:18.29 PuckHeater.configure :default #ambient 22.8C @13:43:18.33 PuckHeater.seek 79.7C @13:44:13.16 PuckHeater.hold 2:00,79.7C @13:46:12.82 Polling Can every 2:00 @13:46:13.36 PuckHeater.hold 30 seconds,78.7C @13:46:43.44 Polling Can every 2:00 @13:46:43.99 PuckHeater.configure :default #ambient 22.8C @13:46:44.03 PuckHeater.hold 30 seconds,78.1C @13:47:14.10 Polling Can every 2:00 @13:47:14.65 PuckHeater.hold 10:00,77.6C @13:57:12.29 Lysis complete. Syringe pressure @16.9psi @13:57:12.31 Archive syringe pumping up to 5 strokes to reach 40psi Archive stroke #1 of 5 @13:57:12.38 Archive.in :pressurize @13:57:12.47 Archive.seek :pressurized @13:57:24.94 16.5psi after pressurize @13:57:30.51 16.5psi after pressurize (stable) @13:57:30.52 Archive.in :compress @13:57:30.57 Archive.seek :empty @13:57:38.72 Reached 40.3 of 40.0psi after pumping 1590uL (0.92 strokes) @13:57:38.73 Archive.in :default @13:57:38.78 Archive.hold @13:57:38.84 delay 2 seconds @13:57:40.86 Archive.in :fast @13:57:40.91 Archive.seek :full @13:57:47.57 Lysis1.seek 138uL @13:58:01.43 delay 1 second @13:58:02.45 Archive.seek :full @13:58:02.49 Lysis1.seek :empty @13:58:16.39 Handoff.select :blocked @13:58:16.48 Initial air pressure @45.5psi @13:58:16.54 Lysis1.in :extract @13:58:16.61 Lysis1.seek 600uL,nil @13:58:18.29 Archive syringe pumping up to 6 strokes to reach 25psi @13:58:18.30 Archive stroke #1 of 6 @13:58:18.37 Archive.in :pressurize @13:58:18.43 Archive.seek :pressurized @13:58:30.93 15.2psi after pressurize @13:58:53.58 Archive.in :default @13:58:53.64 Archive.hold @13:58:53.77 Initial 600uL lysate @14.3psi @13:58:53.78 Handoff.select :blocked @13:58:53.86 Initial air pressure @14.5psi @13:58:53.91 Lysis1.seek 825uL,nil @13:58:53.99 Archive syringe pumping up to 6 strokes to reach 17psi @13:58:54.00 Archive stroke #1 of 6 @13:58:54.12 Archive.in :compress @13:58:54.17 Archive.seek :empty @13:59:06.06 Reached 16.9 of 17.0psi after pumping 1671uL (0.97 strokes) @13:59:06.07 Archive.in :default @13:59:06.12 Archive.hold @13:59:06.83 Archive syringe pumping up to 6 strokes to reach 17psi @13:59:06.85 Archive stroke #1 of 6 @13:59:06.96 Archive.in :compress @13:59:07.02 Archive.seek :empty @13:59:09.91 15.1psi after compress @13:59:10.19 Archive.in :default @13:59:10.24 Archive.hold @13:59:10.44 Recovered 825uL lysate @14.0psi @13:59:10.45 Air.in :halfSpeed @13:59:10.50 Air.seek :fullVent @13:59:12.69 Puck Top Vented, bottom @13.3psi @13:59:12.70 Handoff.select :waste @13:59:12.81 delay 5 seconds @13:59:17.87 Handoff open to waste, bottom @0.3psi @13:59:17.89 Air.in :default @13:59:17.94 Air.seek :evac @13:59:19.34 Lysis2.in :DAmix @13:59:19.44 Lysis1.in :DAmix @13:59:19.92 Lysis2.seek 1480uL @13:59:19.95 Lysis1.seek 575uL @13:59:55.01 delay 5 seconds @14:00:00.02 Handoff.select SPR @14:00:00.14 SPR.command "start 2 14" @14:00:01.22 SPR stabilized @14:00:01.34 delay 10 seconds @14:00:11.35 SPR.command "start 1 0" @14:00:14.58 Waiting up to 4:00 for SPR to enter state 1 @14:02:18.87 SPR.command "hold_on" @14:02:20.26 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:02:20.73 Lysis2.seek 1180uL @14:02:20.78 Lysis1.seek 275uL @14:03:02.78 delay 15 seconds @14:03:11.58 Session Begun @14:03:13.13 -> showlog @14:03:17.80 SPR.command "hold_off" @14:03:21.43 Waiting up to 38:00 for SPR to enter state 7 @14:21:21.48 SPRsummary:-11.7RIU,599RIU,98.1RIU,135RIU,none,17.8ng/L,33.8ng/L after 3213s @14:27:58.69 SPR analysis completed @14:28:04.48 Ended log spr23apr1413h.spr.bz2 @14:28:04.49 SPR.command "stats_off" @14:28:04.96 Handoff.select :close @14:28:05.09
Gate.power :heat,:OFF @14:28:05.10 Gate.power :cartridge,:OFF @14:28:05.19 PinPort.in :unlocking @14:28:05.29 PinPort.seek :clear @14:28:05.75 Carousel.in :default @14:28:05.87 Carousel.home.seek 56 @14:28:06.16 Plunge.seek :locked @14:28:07.03 Archive.in :unlocking @14:28:07.17 Archive.seek :clear @14:28:07.25 Air.in :unlocking @14:28:07.37 Air.seek :clear @14:28:07.47 Lysis1.in :unlocking @14:28:07.59 Lysis1.seek :clear @14:28:07.63 Lysis2.in :unlocking @14:28:07.74 Lysis2.seek :clear @14:28:11.72
Gate.power :motor,:OFF @14:28:11.73 Gate.power :core,:OFF @14:28:11.75 Gate.power :analytic1,:OFF @14:28:11.78 Gate.power :analytic2,:OFF @14:28:11.81 Gate.power :analytic3,:OFF @14:28:11.83 Gate.power :main,:OFF @14:28:11.85 Safely stopped and ready to power off @14:28:12.28 Cartridge::None in MAIN -- Not enough dry cartridges remain @14:28:12.33 Session Ended @13:28:11.10PDT24-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:28:11.12 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:28:13.61 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @13:28:14.05 Sea pressure (Abs) tare is 6% of full scale @13:28:19.69 Polling Can every 2:00 @13:28:19.72 Loading /home/esp/esp2/bin/processAll mission @13:28:19.89 Sea pressure (Abs) tare is 6% of full scale @13:28:19.90 Sampling default goal volumes @13:28:19.92 Options: {extraDilutions: [1,1,1]} Cartridge group: standard Spares: {spare: 2} @13:28:19.97 Gate.power :main,:ON,limit to 5000mA @13:28:19.98 Gate.power :analytic1,:ON,limit to 5000mA @13:28:19.99 Gate.power :analytic2,:ON,limit to 5000mA @13:28:20.00 Gate.power :analytic3,:ON,limit to 5000mA @13:28:20.10 Powering core bus @13:28:22.39 SPR Module 1, FW Rev. B, FPGA Rev. C @13:28:22.79
Archive.configure :default @13:28:22.86 Air.configure :default @13:28:22.91 Lysis1.configure :default @13:28:22.96 Lysis2.configure :default @13:28:23.03 Plunge.configure :default @13:28:23.10 SamplePump.configure :default @13:28:23.18 Toroid.configure :default @13:28:23.25 Carousel.configure :default @13:28:23.32 Pressure.configure :default @13:28:23.40 ExtraPump.configure :default @13:28:23.44 PinPort.configure :default @13:28:23.49 All elf controllers running firmware version 4.83 @13:28:23.51 Gate.power :motor,:ON,limit to 5000mA @13:28:23.64 Archive.home.jog 0 @13:28:24.16 Carousel.home.jog 0 @13:28:24.20 Toroid.home.jog 0 @13:28:24.23 Lysis1.home.jog 0 @13:28:24.26 Lysis2.home.jog 0 @13:28:24.29 PinPort.home.jog 0 @13:28:24.31 Air.home.jog 0 @13:28:24.35 Plunge.home.jog 0 @13:28:24.92 Archive.in :homing @13:28:25.09 Archive.jog 1390 @13:28:25.12 Toroid.in :homing @13:28:25.14 Plunge.in :homing @13:28:25.17 Lysis2.in :homing @13:28:25.52 Lysis1.in :homing @13:28:25.59 Air.in :homing @13:28:25.62 PinPort.in :homing @13:28:25.81 Toroid.jog 623 @13:28:25.84 Plunge.jog 1551 @13:28:25.88 Lysis2.jog 1405 @13:28:25.89 Lysis1.jog 162 @13:28:25.92 Air.jog 1391 @13:28:25.94 PinPort.jog -105 @13:28:26.31 PinPort.setRawPosition! 0 @13:28:26.35 PinPort.seek :home @13:28:26.42 PinPort.in :default @13:28:26.48 Lysis1.setRawPosition! 50 @13:28:26.52 Lysis1.seek :home @13:28:26.79 Lysis1.in :default @13:28:26.84 Archive.setRawPosition! 50 @13:28:26.89 Archive.seek :home @13:28:27.16 Archive.in :default @13:28:27.25 Lysis2.setRawPosition! 50 @13:28:27.28 Plunge.setRawPosition! 50 @13:28:27.30 Air.setRawPosition! 50 @13:28:27.38 Lysis2.seek :home @13:28:27.41 Plunge.seek :home @13:28:27.49 Air.seek :home @13:28:27.70 Lysis2.in :default @13:28:27.76 Plunge.in :default @13:28:27.84 Air.in :default @13:28:28.04 Toroid.setRawPosition! 0 @13:28:28.08 Toroid.seek :home @13:28:28.15 Toroid.in :default @13:28:28.23
Toroid.seek :clear @13:28:29.83 Carousel.in :default @13:28:29.94 Carousel.home.seek 11 @13:28:30.21 Selecting Cartridge 55 @13:28:30.44 Carousel.seek 55 @13:28:39.67 Lysis1.in :fast @13:28:39.72 Lysis1.seek :locked @13:28:42.31 Plunge.seek :locked @13:28:42.42 Air.in :fast @13:28:42.48 Air.seek :locked @13:28:44.04
Carousel.reconfigure :locked @13:28:44.16 Powering cartridge bus @13:28:46.77 Gate.setIR 1750,5000,0,6 @13:28:46.88 Gate.setIR 0,5000,0,6 @13:28:46.94 PuckHeater.configure :default @13:28:46.98 Gate.power :heat,:ON,limit to 5000mA @13:28:47.65 Mounted lysis Cartridge board#Q05048 @13:28:47.68 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:28:49.08 SPR.command "read_sequences" @13:28:50.07 SPR.command "stats_on" @13:28:50.75 SPR.command "start 0 1" @13:28:51.47 SPR.start /var/log/esp/spr23apr2413h.spr.bz2 @13:28:53.99 Waiting for baseline SPR stabilization @13:28:54.01 Handoff.select :waste @13:29:00.67 Syringe tare is 18% of full scale @13:29:00.69 PinPort.in :locking @13:29:00.74 PinPort.seek :locked @13:29:01.22 Archive.in :fast @13:29:01.29 Lysis2.in :fast @13:29:01.31 Lysis1.seek 1000uL @13:29:01.44 Lysis2.seek 1000uL @13:29:01.47 Archive.seek :full @13:29:05.99 delayUntil "13:38:53.97" @13:38:53.98 Lysis2.in :DAmix @13:38:54.08 Lysis1.in :DAmix @13:38:54.47 Lysis2.seek 750uL @13:38:54.51 Lysis1.seek 750uL @13:39:29.64 delay 5 seconds @13:39:34.65 Handoff.select SPR @13:39:34.77 SPR.command "start 2 14" @13:39:35.72 SPR stabilized @13:39:35.85 delay 10 seconds @13:39:45.86 SPR.command "start 1 0" @13:39:49.09 Waiting up to 4:00 for SPR to enter state 1 @13:41:53.35 SPR.command "hold_on" @13:41:54.77 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @13:41:55.24 Lysis2.seek 450uL @13:41:55.28 Lysis1.seek 450uL @13:42:37.32 delay 15 seconds @13:42:52.34 SPR.command "hold_off" @13:42:55.42 Waiting up to 38:00 for SPR to enter state 7 @14:00:55.00 SPRsummary:9.02RIU,215RIU,184RIU,266RIU after 2031s @14:07:32.33 delay 10 seconds @14:07:42.34 SPR.command "start 1 0" @14:07:45.10 Waiting up to 4:00 for SPR to enter state 1 @14:09:49.37 SPR.command "hold_on" @14:09:50.78 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:09:51.23 Lysis2.seek 300uL @14:09:51.28 Lysis1.seek 300uL @14:10:12.37 delay 15 seconds @14:10:27.39 SPR.command "hold_off" @14:10:30.62 Waiting up to 38:00 for SPR to enter state 7 @14:35:07.53 delay 10 seconds @14:35:17.55 SPR.command "start 1 0" @14:35:20.31 Waiting up to 4:00 for SPR to enter state 1 @14:37:24.57 SPR.command "hold_on" @14:37:26.47 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:37:26.89 Lysis2.seek 150uL @14:37:26.95 Lysis1.seek 150uL @14:37:48.01 delay 15 seconds @14:38:03.02 SPR.command "hold_off" @14:38:06.31 Waiting up to 38:00 for SPR to enter state 7 @15:02:42.74 delay 10 seconds @15:02:52.76 SPR.command "start 1 0" @15:02:55.51 Waiting up to 4:00 for SPR to enter state 1 @15:04:59.77 SPR.command "hold_on" @15:05:01.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:05:01.61 Lysis2.seek 0uL @15:05:01.68 Lysis1.seek 0uL @15:05:22.74 delay 15 seconds @15:05:37.75 SPR.command "hold_off" @15:05:41.03 Waiting up to 38:00 for SPR to enter state 7 @15:30:17.84 SPR analysis completed @15:30:19.69 Ended log spr23apr2413h.spr.bz2 @15:30:19.71 SPR.command "stats_off" @15:30:20.80 Handoff.select :close @15:30:20.92
Gate.power :heat,:OFF @15:30:20.93 Gate.power :cartridge,:OFF @15:30:20.99 PinPort.in :unlocking @15:30:21.04 PinPort.seek :clear @15:30:21.49 Carousel.in :default @15:30:21.92 Carousel.home.seek 55 @15:30:22.20 Plunge.seek :locked @15:30:22.33 Archive.in :unlocking @15:30:22.45 Archive.seek :clear @15:30:22.49 Air.in :unlocking @15:30:22.64 Air.seek :clear @15:30:22.70 Lysis1.in :unlocking @15:30:22.84 Lysis2.in :unlocking @15:30:22.87 Lysis1.seek :clear @15:30:22.96 Lysis2.seek :clear @15:30:27.51
Gate.power :motor,:OFF @15:30:27.53 Gate.power :core,:OFF @15:30:27.54 Gate.power :analytic1,:OFF @15:30:27.55 Gate.power :analytic2,:OFF @15:30:27.56 Gate.power :analytic3,:OFF @15:30:27.57 Gate.power :main,:OFF Safely stopped and ready to power off @15:30:31.34 Gate.power :main,:ON,limit to 5000mA @15:30:31.36 Gate.power :analytic1,:ON,limit to 5000mA @15:30:31.37 Gate.power :analytic2,:ON,limit to 5000mA @15:30:31.38 Gate.power :analytic3,:ON,limit to 5000mA @15:30:31.43 Powering core bus @15:30:33.79 SPR Module 1, FW Rev. B, FPGA Rev. C @15:30:34.13
Archive.configure :default @15:30:34.20 Air.configure :default @15:30:34.26 Lysis1.configure :default @15:30:34.31 Lysis2.configure :default @15:30:34.38 Plunge.configure :default @15:30:34.45 SamplePump.configure :default @15:30:34.53 Toroid.configure :default @15:30:34.60 Carousel.configure :default @15:30:34.67 Pressure.configure :default @15:30:34.74 ExtraPump.configure :default @15:30:34.79 PinPort.configure :default @15:30:34.84 All elf controllers running firmware version 4.83 @15:30:34.86 Gate.power :motor,:ON,limit to 5000mA @15:30:35.14 Archive.home.jog 0 @15:30:35.56 Carousel.home.jog 0 @15:30:35.60 Toroid.home.jog 0 @15:30:35.63 Lysis1.home.jog 0 @15:30:35.67 Lysis2.home.jog 0 @15:30:35.70 PinPort.home.jog 0 @15:30:35.73 Air.home.jog 0 @15:30:35.80 Plunge.home.jog 0 @15:30:36.30 Archive.in :homing @15:30:36.58 Archive.jog 1382 @15:30:36.63 Toroid.in :homing @15:30:36.64 Plunge.in :homing @15:30:36.67 Lysis2.in :homing @15:30:36.70 Lysis1.in :homing @15:30:36.73 Air.in :homing @15:30:36.76 PinPort.in :homing @15:30:36.96 Plunge.jog 1539 @15:30:37.00 Toroid.jog 625 @15:30:37.08 PinPort.jog -106 @15:30:37.47 Lysis2.jog 1404 @15:30:37.55 Lysis1.jog 150 @15:30:37.58 Air.jog 1383 @15:30:37.84 PinPort.setRawPosition! 0 @15:30:37.87 PinPort.seek :home @15:30:37.95 Archive.setRawPosition! 50 @15:30:37.97 PinPort.in :default @15:30:38.05 Archive.seek :home @15:30:38.15 Lysis1.setRawPosition! 50 @15:30:38.19 Lysis1.seek :home @15:30:38.30 Plunge.setRawPosition! 50 @15:30:38.35 Plunge.seek :home @15:30:38.42 Archive.in :default @15:30:38.49 Lysis1.in :default @15:30:38.61 Plunge.in :default @15:30:38.85 Lysis2.setRawPosition! 50 @15:30:38.91 Lysis2.seek :home @15:30:38.92 Air.setRawPosition! 50 @15:30:39.00 Air.seek :home @15:30:39.20 Lysis2.in :default @15:30:39.27 Air.in :default @15:30:39.71 Toroid.setRawPosition! 0 @15:30:39.75 Toroid.seek :home @15:30:39.81 Toroid.in :default @15:30:39.87
Toroid.seek :clear @15:30:41.47 Carousel.in :default @15:30:41.57 Carousel.home.seek 55 @15:30:41.80 Selecting Cartridge 54 @15:30:42.03 Carousel.seek 54 @15:30:42.49 Lysis1.in :fast @15:30:42.54 Lysis1.seek :locked @15:30:45.12 Plunge.seek :locked @15:30:45.24 Air.in :fast @15:30:45.29 Air.seek :locked @15:30:46.85
Carousel.reconfigure :locked @15:30:46.97 Powering cartridge bus @15:30:49.58 Gate.setIR 1750,5000,0,6 @15:30:49.69 Gate.setIR 0,5000,0,6 @15:30:49.74 PuckHeater.configure :default @15:30:49.79 Gate.power :heat,:ON,limit to 5000mA @15:30:50.46 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @15:30:50.48 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:30:51.89 SPR.command "read_sequences" @15:30:52.58 SPR.command "stats_on" @15:30:52.70 SPR.command "start 0 1" @15:30:53.43 SPR.start /var/log/esp/spr23apr2415h.spr.bz2 @15:30:55.92 Waiting for baseline SPR stabilization @15:30:55.93 Handoff.select :waste @15:31:03.05 Syringe tare is 18% of full scale @15:31:03.07 PinPort.in :locking @15:31:03.11 PinPort.seek :locked @15:31:03.58 Archive.in :fast @15:31:03.62 Lysis2.in :fast @15:31:03.69 Lysis1.seek 1000uL @15:31:03.73 Archive.seek :full @15:31:03.84 Lysis2.seek 1000uL @15:31:08.34 delayUntil "15:40:55.90" @15:40:55.91 Lysis2.in :DAmix @15:40:56.01 Lysis1.in :DAmix @15:40:56.39 Lysis2.seek 750uL @15:40:56.44 Lysis1.seek 750uL @15:41:31.56 delay 5 seconds @15:41:36.57 Handoff.select SPR @15:41:36.69 SPR.command "start 2 14" @15:41:37.65 SPR stabilized @15:41:37.77 delay 10 seconds @15:41:47.79 SPR.command "start 1 0" @15:41:51.50 Waiting up to 4:00 for SPR to enter state 1 @15:43:56.23 SPR.command "hold_on" @15:43:57.64 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @15:43:58.09 Lysis2.seek 450uL @15:43:58.14 Lysis1.seek 450uL @15:44:40.18 delay 15 seconds @15:44:55.19 SPR.command "hold_off" @15:44:58.29 Waiting up to 38:00 for SPR to enter state 7 @16:02:57.88 SPRsummary:3.77RIU,218RIU,168RIU,292RIU after 2032s @16:09:35.21 delay 10 seconds @16:09:45.23 SPR.command "start 1 0" @16:09:47.98 Waiting up to 4:00 for SPR to enter state 1 @16:11:52.25 SPR.command "hold_on" @16:11:53.66 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:11:54.16 Lysis2.seek 300uL @16:11:54.19 Lysis1.seek 300uL @16:12:15.26 delay 15 seconds @16:12:30.28 SPR.command "hold_off" @16:12:33.50 Waiting up to 38:00 for SPR to enter state 7 @16:37:10.44 delay 10 seconds @16:37:20.46 SPR.command "start 1 0" @16:37:23.19 Waiting up to 4:00 for SPR to enter state 1 @16:39:27.47 SPR.command "hold_on" @16:39:28.86 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:39:29.31 Lysis2.seek 150uL @16:39:29.37 Lysis1.seek 150uL @16:39:50.46 delay 15 seconds @16:40:05.48 SPR.command "hold_off" @16:40:08.71 Waiting up to 38:00 for SPR to enter state 7 @17:04:45.65 delay 10 seconds @17:04:55.67 SPR.command "start 1 0" @17:04:58.91 Waiting up to 4:00 for SPR to enter state 1 @17:07:03.63 SPR.command "hold_on" @17:07:05.53 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:07:05.99 Lysis2.seek 0uL @17:07:06.04 Lysis1.seek 0uL @17:07:27.09 delay 15 seconds @17:07:42.11 SPR.command "hold_off" @17:07:44.86 Waiting up to 38:00 for SPR to enter state 7 @17:32:20.75 SPR analysis completed @17:32:22.53 Ended log spr23apr2415h.spr.bz2 @17:32:22.54 SPR.command "stats_off" @17:32:23.34 Handoff.select :close @17:32:23.46
Gate.power :heat,:OFF @17:32:23.47 Gate.power :cartridge,:OFF @17:32:23.53 PinPort.in :unlocking @17:32:23.58 PinPort.seek :clear @17:32:24.04 Carousel.in :default @17:32:24.14 Carousel.home.seek 54 @17:32:24.41 Plunge.seek :locked @17:32:24.53 Archive.in :unlocking @17:32:24.66 Archive.seek :clear @17:32:24.69 Air.in :unlocking @17:32:24.86 Lysis1.in :unlocking @17:32:24.89 Air.seek :clear @17:32:25.04 Lysis1.seek :clear @17:32:25.09 Lysis2.in :unlocking @17:32:25.18 Lysis2.seek :clear @17:32:29.72
Gate.power :motor,:OFF @17:32:29.74 Gate.power :core,:OFF @17:32:29.75 Gate.power :analytic1,:OFF @17:32:29.76 Gate.power :analytic2,:OFF @17:32:29.77 Gate.power :analytic3,:OFF @17:32:29.78 Gate.power :main,:OFF Safely stopped and ready to power off @17:32:29.96 Cartridge::None in MAIN -- Not enough standard cartridges remain @15:02:30.45PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:02:30.47 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:02:32.90 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:02:33.36 Sea pressure (Abs) tare is 6% of full scale @15:02:39.15 Polling Can every 2:00 @15:02:39.18 Loading /home/esp/esp2/bin/processAll mission @15:02:39.34 Sea pressure (Abs) tare is 6% of full scale @15:02:39.35 Sampling maximum of 445.8ml per cartridge @15:02:39.36 Cartridge group: dry Spares: {spare: 2} @15:02:39.49 Gate.power :analytic1,:ON,limit to 5000mA @15:02:39.50 Gate.power :analytic2,:ON,limit to 5000mA @15:02:39.51 Gate.power :analytic3,:ON,limit to 5000mA @15:02:40.09 Archive.configure :default @15:02:40.22 Air.configure :default @15:02:40.27 SPR Module 1, FW Rev. B, FPGA Rev. C @15:02:40.30
Air.hold @15:02:40.36 Air.configure :default @15:02:40.46 Lysis1.configure :default @15:02:40.51 Lysis1.hold @15:02:40.56 Lysis1.configure :default @15:02:40.66 Lysis2.configure :default @15:02:40.79 Plunge.configure :default @15:02:40.83 Plunge.hold @15:02:40.89 Plunge.configure :default @15:02:41.01 SamplePump.configure :default @15:02:41.13 Toroid.configure :default @15:02:41.18 Toroid.hold @15:02:41.23 Toroid.configure :default @15:02:41.36 Carousel.configure :default @15:02:41.40 Carousel.hold @15:02:41.46 Carousel.configure :default @15:02:41.58 Pressure.configure :default @15:02:41.72 ExtraPump.configure :default @15:02:41.82 PinPort.configure :default @15:02:41.87 All elf controllers running firmware version 4.83 @15:02:42.85 Toroid.seek :clear @15:02:42.91 Carousel.in :default @15:02:43.01 Carousel.home.seek 5 @15:02:43.25 Selecting Cartridge 5 @15:02:43.47 Carousel.seek 5 @15:02:43.65 Lysis1.in :fast @15:02:43.70 Lysis1.seek :locked @15:02:46.28 Plunge.seek :locked @15:02:46.40 Air.in :fast @15:02:46.45 Air.seek :locked @15:02:48.01
Carousel.reconfigure :locked @15:02:48.14 Powering cartridge bus @15:02:50.75 Gate.setIR 1750,5000,0,6 @15:02:50.86 Gate.setIR 0,5000,0,6 @15:02:50.91 Gate.power :cartridge,:OFF @15:02:51.44 Mounted archive Cartridge @15:02:51.71 Preparing to filter cartridge 5 @15:02:51.94 Priming sample loop w/100ml, bypass w/10ml @15:02:51.95 SamplePump.setPosition! 0ml @15:02:51.98 Exhaust.close @15:02:52.04 Bypass.open @15:02:53.53 Intake.open @15:02:54.23 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:02:56.81 Vacuum tare is 60% of full scale @15:02:56.93 Pressure.in :default @15:02:56.98 Pressure.hold @15:02:57.15 SamplePump.in :prime @15:02:57.20 SamplePump.seek 10ml,59 seconds @15:03:05.93 Pressure.stop @15:03:05.97 SamplePump.setPosition! 0ml @15:03:06.01 Exhaust.open @15:03:07.46 Bypass.close @15:03:09.03 Pressure.in :default @15:03:09.08 Pressure.hold @15:03:09.32 SamplePump.in :prime @15:03:09.37 SamplePump.seek 100ml,5:16 @15:04:39.42 Pressure.stop @15:04:39.48 Bypass.open @15:04:40.17 Intake.close @15:04:40.90 Toroid.in :hold @15:04:40.95 Toroid.seek :crack @15:04:42.48 Toroid.seek :cartridge @15:04:43.57 Toroid.in :default @15:04:43.62 Toroid.seek :clear @15:04:45.56 Pressurizing cartridge to 26psi above 14.9psia @15:04:45.58 SamplePump.setPosition! 0ml @15:04:45.61 Intake.open @15:04:46.24 Bypass.close @15:04:46.93 Exhaust.close @15:04:47.76 Pressure.in :default @15:04:47.81 Pressure.hold @15:04:47.98 SamplePump.in :charge @15:04:48.03 SamplePump.seek 2.3ml,42 seconds @15:04:50.59 Pressure.stop @15:04:50.63 Intake.close @15:04:51.36 Pressurized cartridge with 0.029ml to 26.1psi @15:04:51.38 Isolated @35.7psia (20.8psi) @15:04:51.39 Verifying cartridge holds at least 0.65..0.85 of 35.7psia @15:04:51.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:04:54.57 Toroid.in :hold @15:04:54.63 Toroid.seek :bypass @15:04:56.25 Toroid.in :default @15:04:56.30 Toroid.seek :clear @15:04:57.98 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 42% to 20.9psia @15:04:57.99 Retry #1 of 2 @15:04:58.02 Cmd::SpareCartridge in FILTERING -- 1 spare remaining @15:04:58.05 Cartridge::None in FILTERING -- No dry cartridges remain @15:04:58.06 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:06:32.30 Session Begun @15:06:36.18 -> slot {[4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 5 => { Type: :archive_bac, State: :leaky}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @15:06:56.21 -> Cartridge.unlock @15:06:56.30 Carousel.in :default @15:06:56.42 Carousel.home.seek 5 @15:06:56.79 Air.in :unlocking @15:06:56.92 Air.seek :clear @15:06:58.26 Lysis1.in :unlocking @15:06:58.40 Plunge.in :unlocking @15:06:58.44 Lysis1.seek :clear @15:06:58.55 Plunge.seek :clear @15:07:13.77 -> slot 5 =>[:dry, :archive_bac] {5 => { Type: :archive_bac, State: :dry}} @15:07:28.50 -> MainThread.exit @15:07:28.58
SamplePump.stop # @15:07:28.69 ThreadError in bill -- killed thread @15:07:28.74 Session Ended @15:07:45.81PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:07:45.83 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:07:48.26 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:07:48.72 Sea pressure (Abs) tare is 6% of full scale @15:07:54.47 Polling Can every 2:00 @15:07:54.50 Loading /home/esp/esp2/bin/processAll mission @15:07:54.67 Sea pressure (Abs) tare is 6% of full scale Sampling maximum of 554.8ml per cartridge @15:07:54.68 Cartridge group: dry Spares: {spare: 1} @15:07:54.81 Gate.power :analytic1,:ON,limit to 5000mA @15:07:54.82 Gate.power :analytic2,:ON,limit to 5000mA @15:07:54.83 Gate.power :analytic3,:ON,limit to 5000mA @15:07:55.33 SPR Module 1, FW Rev. B, FPGA Rev. C @15:07:55.47
Archive.configure :default @15:07:55.52 Archive.hold @15:07:55.57 Archive.configure :default @15:07:55.69 Air.configure :default @15:07:55.74 Air.hold @15:07:55.79 Air.configure :default @15:07:55.90 Lysis1.configure :default @15:07:55.96 Lysis1.hold @15:07:56.01 Lysis1.configure :default @15:07:56.11 Lysis2.configure :default @15:07:56.16 Lysis2.hold @15:07:56.21 Lysis2.configure :default @15:07:56.33 Plunge.configure :default @15:07:56.38 Plunge.hold @15:07:56.43 Plunge.configure :default @15:07:56.55 SamplePump.configure :default @15:07:56.67 Toroid.configure :default @15:07:56.72 Toroid.hold @15:07:56.77 Toroid.configure :default @15:07:56.90 Carousel.configure :default @15:07:56.95 Carousel.hold @15:07:57.00 Carousel.configure :default @15:07:57.13 Pressure.configure :default @15:07:57.57 ExtraPump.configure :default @15:07:57.72 PinPort.configure :default @15:07:57.77 PinPort.hold @15:07:57.82 PinPort.configure :default @15:07:57.87 All elf controllers running firmware version 4.83 @15:07:58.50 Toroid.seek :clear @15:07:58.56 Carousel.in :default @15:07:58.66 Carousel.home.seek 5 @15:07:58.89 Selecting Cartridge 5 @15:07:59.12 Carousel.seek 5 @15:07:59.30 Lysis1.in :fast @15:07:59.35 Lysis1.seek :locked @15:08:01.92 Plunge.seek :locked @15:08:02.05 Air.in :fast @15:08:02.10 Air.seek :locked @15:08:03.66
Carousel.reconfigure :locked @15:08:03.78 Powering cartridge bus @15:08:06.39 Gate.setIR 1750,5000,0,6 @15:08:06.51 Gate.setIR 0,5000,0,6 @15:08:06.55 Gate.power :cartridge,:OFF @15:08:07.08 Mounted archive Cartridge @15:08:07.44 Preparing to filter cartridge 5 @15:08:07.67 Priming sample loop w/100ml, bypass w/10ml @15:08:07.68 SamplePump.setPosition! 0ml @15:08:07.71 Exhaust.close @15:08:07.77 Bypass.open @15:08:09.23 Intake.open @15:08:09.95 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:08:12.53 Vacuum tare is 60% of full scale @15:08:12.65 Pressure.in :default @15:08:12.70 Pressure.hold @15:08:12.87 SamplePump.in :prime @15:08:13.30 SamplePump.seek 10ml,59 seconds @15:08:22.03 Pressure.stop @15:08:22.07 SamplePump.setPosition! 0ml @15:08:22.11 Exhaust.open @15:08:23.58 Bypass.close @15:08:25.09 Pressure.in :default @15:08:25.14 Pressure.hold @15:08:25.37 SamplePump.in :prime @15:08:25.42 SamplePump.seek 100ml,5:16 @15:09:55.09 Pressure.stop @15:09:55.15 Bypass.open @15:09:55.82 Intake.close @15:09:56.54 Toroid.in :hold @15:09:56.59 Toroid.seek :crack @15:09:58.11 Toroid.seek :cartridge @15:09:59.21 Toroid.in :default @15:09:59.26 Toroid.seek :clear @15:10:01.21 Pressurizing cartridge to 26psi above 14.9psia @15:10:01.22 SamplePump.setPosition! 0ml @15:10:01.25 Intake.open @15:10:01.90 Bypass.close @15:10:02.56 Exhaust.close @15:10:03.41 Pressure.in :default @15:10:03.46 Pressure.hold @15:10:03.63 SamplePump.in :charge @15:10:03.69 SamplePump.seek 2.3ml,42 seconds @15:10:06.27 Pressure.stop @15:10:06.32 Intake.close @15:10:07.05 Pressurized cartridge with 0.030ml to 26.1psi @15:10:07.06 Isolated @31.1psia (16.2psi) @15:10:07.07 Verifying cartridge holds at least 0.65..0.85 of 31.1psia @15:10:07.09 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:10:09.76 Toroid.in :hold @15:10:09.81 Toroid.seek :bypass @15:10:11.43 Toroid.in :default @15:10:11.49 Toroid.seek :clear @15:10:13.16 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 39% to 19.1psia @15:10:13.17 Retry #1 of 2 @15:10:13.20 Cmd::SpareCartridge in FILTERING -- 0 spares remaining @15:10:13.23 Cartridge::None in FILTERING -- No dry cartridges remain @15:10:13.24 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:16:11.28 Session Begun @15:16:19.34 -> progress >>> Thread[:MAIN] is sleeping <<< @15:07:54 Cartridge group: dry Spares: {spare: 1} @15:07:58 Selecting Cartridge 5 >>> Thread[:FILTERING] is suspended <<< @15:08:07 Preparing to filter cartridge 5 @15:08:07 Priming sample loop w/100ml, bypass w/10ml @15:08:09 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:10:01 Pressurizing cartridge to 26psi above 14.9psia @15:10:03 Sample Pump (SP) pressureOutOfBounds at 0.03ml @15:10:07 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:17:21.47 -> Cmd.resume @15:17:21.49 Resuming Thread (suspended since 15:08:07) @15:17:21.52 Preparing to filter cartridge 5 @15:17:21.75 Bypass.open @15:17:22.54 Toroid.in :hold @15:17:23.01 Toroid.seek :crack @15:17:24.56 Toroid.seek :cartridge @15:17:25.66 Toroid.in :default @15:17:25.73 Toroid.seek :clear @15:17:27.53 Exhaust.open @15:17:28.44 Pressurizing cartridge to 26psi above 14.7psia @15:17:28.46 SamplePump.setPosition! 0ml @15:17:28.52 Intake.open @15:17:29.16 Bypass.close @15:17:29.86 Exhaust.close @15:17:30.71 Pressure.in :default @15:17:30.77 Pressure.hold @15:17:30.97 SamplePump.seek 2.3ml,42 seconds @15:17:33.57 Pressure.stop @15:17:33.63 Intake.close @15:17:34.35 Pressurized cartridge with 0.031ml to 26.1psi @15:17:34.37 Isolated @33.0psia (18.3psi) @15:17:34.39 Verifying cartridge holds at least 0.65..0.85 of 33.0psia @15:17:34.42 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:17:36.61 Toroid.in :hold @15:17:36.68 Toroid.seek :bypass @15:17:38.32 Toroid.in :default @15:17:38.39 Toroid.seek :clear @15:17:40.08 Cartridge::Sampler::Leaky in FILTERING -- Isolated cartridge pressure fell by 36% to 21.1psia @15:17:40.09 Retry #1 of 2 @15:17:40.12 Cmd::Error in FILTERING -- No spare cartridges remain @15:17:40.14 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:17:59.77 Cmd::Error in bill -- Not Processing @15:17:59.82 UserAbort in bill -- User Abort!! @15:18:02.36 -> slot {[4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 5 => { Type: :archive_bac, State: :leaky}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @15:18:23.98 -> slot 5 =>[:dry, :archive_bac] {5 => { Type: :archive_bac, State: :dry}} @15:18:32.21 -> Cartridge.unlock @15:18:32.30 Carousel.in :default @15:18:32.42 Carousel.home.seek 5 @15:18:32.79 Air.in :unlocking @15:18:32.92 Air.seek :clear @15:18:34.29 Lysis1.in :unlocking @15:18:34.43 Plunge.in :unlocking @15:18:34.47 Lysis1.seek :clear @15:18:34.58 Plunge.seek :clear @15:18:39.94 -> exit @15:18:39.96 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @15:19:23.02 Session Begun @15:19:27.58 -> MainThread.exit @15:19:27.67
SamplePump.stop # @15:19:27.78 Session Ended @15:19:45.66PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:19:45.68 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:19:48.16 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:19:48.61 Sea pressure (Abs) tare is 6% of full scale @15:19:54.40 Polling Can every 2:00 @15:19:54.44 Loading /home/esp/esp2/bin/processAll mission @15:19:54.60 Sea pressure (Abs) tare is 6% of full scale @15:19:54.61 Sampling maximum of 445.8ml per cartridge @15:19:54.62 Cartridge group: dry Spares: {spare: 0} @15:19:54.75 Gate.power :analytic1,:ON,limit to 5000mA @15:19:54.76 Gate.power :analytic2,:ON,limit to 5000mA @15:19:54.77 Gate.power :analytic3,:ON,limit to 5000mA @15:19:55.35 Archive.configure :default @15:19:55.40 Archive.hold @15:19:55.46 Archive.configure :default @15:19:55.58 Air.configure :default @15:19:55.63 Air.hold @15:19:55.68 Air.configure :default @15:19:55.79 Lysis1.configure :default @15:19:55.83 Lysis1.hold @15:19:55.90 Lysis1.configure :default @15:19:55.96 SPR Module 1, FW Rev. B, FPGA Rev. C @15:19:56.05
Lysis2.configure :default @15:19:56.09 Lysis2.hold @15:19:56.14 Lysis2.configure :default @15:19:56.26 Plunge.configure :default @15:19:56.31 Plunge.hold @15:19:56.37 Plunge.configure :default @15:19:56.49 SamplePump.configure :default @15:19:56.61 Toroid.configure :default @15:19:56.65 Toroid.hold @15:19:56.71 Toroid.configure :default @15:19:56.83 Carousel.configure :default @15:19:56.88 Carousel.hold @15:19:56.95 Carousel.configure :default @15:19:57.08 Pressure.configure :default @15:19:57.54 ExtraPump.configure :default @15:19:57.64 PinPort.configure :default @15:19:57.70 PinPort.hold @15:19:57.76 PinPort.configure :default @15:19:57.81 All elf controllers running firmware version 4.83 @15:19:58.40 Toroid.seek :clear @15:19:58.46 Carousel.in :default @15:19:58.56 Carousel.home.seek 5 @15:19:58.79 Selecting Cartridge 5 @15:19:59.02 Carousel.seek 5 @15:19:59.19 Lysis1.in :fast @15:19:59.24 Lysis1.seek :locked @15:20:01.81 Plunge.seek :locked @15:20:01.94 Air.in :fast @15:20:02.00 Air.seek :locked @15:20:03.55
Carousel.reconfigure :locked @15:20:03.67 Powering cartridge bus @15:20:06.29 Gate.setIR 1750,5000,0,6 @15:20:06.40 Gate.setIR 0,5000,0,6 @15:20:06.45 Gate.power :cartridge,:OFF @15:20:06.98 Mounted archive Cartridge @15:20:07.23 Preparing to filter cartridge 5 @15:20:07.47 Priming sample loop w/100ml, bypass w/10ml @15:20:07.48 SamplePump.setPosition! 0ml @15:20:07.51 Exhaust.close @15:20:07.57 Bypass.open @15:20:09.02 Intake.open @15:20:09.72 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:20:12.29 Vacuum tare is 60% of full scale @15:20:12.40 Pressure.in :default @15:20:12.45 Pressure.hold @15:20:13.01 SamplePump.in :prime @15:20:13.06 SamplePump.seek 10ml,59 seconds @15:20:21.80 Pressure.stop @15:20:21.83 SamplePump.setPosition! 0ml @15:20:21.87 Exhaust.open @15:20:23.33 Bypass.close @15:20:24.86 Pressure.in :default @15:20:24.91 Pressure.hold @15:20:25.14 SamplePump.in :prime @15:20:25.20 SamplePump.seek 100ml,5:16 @15:21:54.63 Pressure.stop @15:21:54.69 Bypass.open @15:21:55.41 Intake.close @15:21:56.14 Toroid.in :hold @15:21:56.19 Toroid.seek :crack @15:21:57.70 Toroid.seek :cartridge @15:21:58.80 Toroid.in :default @15:21:58.85 Toroid.seek :clear @15:22:00.80 Pressurizing cartridge to 26psi above 15.0psia @15:22:00.81 SamplePump.setPosition! 0ml @15:22:00.84 Intake.open @15:22:01.51 Bypass.close @15:22:02.22 Exhaust.close @15:22:03.06 Pressure.in :default @15:22:03.11 Pressure.hold @15:22:03.28 SamplePump.in :charge @15:22:03.33 SamplePump.seek 2.3ml,42 seconds @15:22:06.12 Pressure.stop @15:22:06.17 Intake.close @15:22:06.90 Pressurized cartridge with 0.036ml to 26.0psi @15:22:06.91 Isolated @23.3psia (8.3psi) @15:22:06.92 Verifying cartridge holds at least 0.65..0.85 of 23.3psia @15:22:06.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:22:11.52 Cartridge pressure fell 24% to 17.6psia @15:22:11.62 Toroid.in :hold @15:22:11.68 Toroid.seek :bypass @15:22:13.30 Toroid.in :default @15:22:13.35 Toroid.seek :clear @15:22:15.02 Cartridge::Sampler::Leaky in FILTERING -- Retained only 17.6psia @15:22:15.03 Retry #1 of 2 @15:22:15.04 Cmd::Error in FILTERING -- No spare cartridges remain @15:22:15.05 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:23:57.18 Session Begun @15:24:02.89 -> Cmd.resume -1 @15:24:02.91 Resuming Thread (suspended since 15:22:06) @15:24:02.94 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:24:07.54 Cartridge pressure fell 25% to 17.4psia @15:24:07.71 Cartridge::Sampler::Leaky in FILTERING -- Retained only 17.4psia @15:24:07.72 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:24:39.15 Cmd::Error in bill -- Not Processing @15:24:39.21 UserAbort in bill -- User Abort!! @15:24:44.02 -> slot {[4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 5 => { Type: :archive_bac, State: :leaky}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @15:24:58.98 -> slot 5 =>[:dry, :archive_bac] {5 => { Type: :archive_bac, State: :dry}} @15:25:08.20 -> Cartridge.unlock @15:25:08.29 Carousel.in :default @15:25:08.41 Carousel.home.seek 5 @15:25:08.77 Air.in :unlocking @15:25:08.87 Air.seek :clear @15:25:10.19 Lysis1.in :unlocking @15:25:10.33 Lysis1.seek :clear @15:25:10.37 Plunge.in :unlocking @15:25:10.48 Plunge.seek :clear @15:25:18.27 -> MainThread.exit @15:25:18.36
SamplePump.stop # @15:25:18.47 ThreadError in bill -- killed thread @15:25:18.50 Session Ended @15:25:32.06PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:25:32.08 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:25:34.64 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:25:35.11 Sea pressure (Abs) tare is 6% of full scale @15:25:41.04 Polling Can every 2:00 @15:25:41.08 Loading /home/esp/esp2/bin/processAll mission @15:25:41.25 Sea pressure (Abs) tare is 6% of full scale @15:25:41.26 Sampling maximum of 445.8ml per cartridge @15:25:41.27 Cartridge group: dry Spares: {spare: 0} @15:25:41.40 Gate.power :analytic1,:ON,limit to 5000mA @15:25:41.41 Gate.power :analytic2,:ON,limit to 5000mA @15:25:41.42 Gate.power :analytic3,:ON,limit to 5000mA @15:25:42.00 Archive.configure :default @15:25:42.05 Archive.hold @15:25:42.13 SPR Module 1, FW Rev. B, FPGA Rev. C @15:25:42.15
Archive.configure :default @15:25:42.27 Air.configure :default @15:25:42.31 Air.hold @15:25:42.37 Air.configure :default @15:25:42.47 Lysis1.configure :default @15:25:42.52 Lysis1.hold @15:25:42.58 Lysis1.configure :default @15:25:42.68 Lysis2.configure :default @15:25:42.73 Lysis2.hold @15:25:42.78 Lysis2.configure :default @15:25:42.90 Plunge.configure :default @15:25:42.94 Plunge.hold @15:25:43.00 Plunge.configure :default @15:25:43.12 SamplePump.configure :default @15:25:43.24 Toroid.configure :default @15:25:43.29 Toroid.hold @15:25:43.34 Toroid.configure :default @15:25:43.46 Carousel.configure :default @15:25:43.51 Carousel.hold @15:25:43.58 Carousel.configure :default @15:25:43.70 Pressure.configure :default @15:25:44.18 ExtraPump.configure :default @15:25:44.28 PinPort.configure :default @15:25:44.33 PinPort.hold @15:25:44.38 PinPort.configure :default @15:25:44.43 All elf controllers running firmware version 4.83 @15:25:45.02 Toroid.seek :clear @15:25:45.08 Carousel.in :default @15:25:45.18 Carousel.home.seek 5 @15:25:45.41 Selecting Cartridge 5 @15:25:45.64 Carousel.seek 5 @15:25:45.82 Lysis1.in :fast @15:25:45.87 Lysis1.seek :locked @15:25:48.45 Plunge.seek :locked @15:25:48.58 Air.in :fast @15:25:48.63 Air.seek :locked @15:25:50.19
Carousel.reconfigure :locked @15:25:50.31 Powering cartridge bus @15:25:52.93 Gate.setIR 1750,5000,0,6 @15:25:53.04 Gate.setIR 0,5000,0,6 @15:25:53.09 Gate.power :cartridge,:OFF @15:25:53.62 Mounted archive Cartridge @15:25:53.88 Preparing to filter cartridge 5 @15:25:54.12 Priming sample loop w/100ml, bypass w/10ml @15:25:54.14 SamplePump.setPosition! 0ml @15:25:54.17 Exhaust.close @15:25:54.23 Bypass.open @15:25:55.73 Intake.open @15:25:56.41 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:25:58.99 Vacuum tare is 60% of full scale @15:25:59.11 Pressure.in :default @15:25:59.16 Pressure.hold @15:25:59.70 SamplePump.in :prime @15:25:59.75 SamplePump.seek 10ml,59 seconds @15:26:08.48 Pressure.stop @15:26:08.52 SamplePump.setPosition! 0ml @15:26:08.56 Exhaust.open @15:26:10.02 Bypass.close @15:26:11.53 Pressure.in :default @15:26:11.58 Pressure.hold @15:26:11.80 SamplePump.in :prime @15:26:11.86 SamplePump.seek 100ml,5:16 @15:27:41.25 Pressure.stop @15:27:41.33 Bypass.open @15:27:42.03 Intake.close @15:27:42.75 Toroid.in :hold @15:27:42.80 Toroid.seek :crack @15:27:44.32 Toroid.seek :cartridge @15:27:45.41 Toroid.in :default @15:27:45.46 Toroid.seek :clear @15:27:47.42 Pressurizing cartridge to 26psi above 14.9psia @15:27:47.43 SamplePump.setPosition! 0ml @15:27:47.46 Intake.open @15:27:48.08 Bypass.close @15:27:48.80 Exhaust.close @15:27:49.61 Pressure.in :default @15:27:49.67 Pressure.hold @15:27:49.84 SamplePump.in :charge @15:27:49.90 SamplePump.seek 2.3ml,42 seconds @15:27:52.65 Pressure.stop @15:27:52.70 Intake.close @15:27:53.42 Pressurized cartridge with 0.034ml to 26.1psi @15:27:53.43 Isolated @25.6psia (10.8psi) @15:27:53.44 Verifying cartridge holds at least 0.65..0.85 of 25.6psia @15:27:53.46 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:27:58.53 Cartridge pressure fell 32% to 17.4psia @15:27:58.64 Toroid.in :hold @15:27:58.70 Toroid.seek :bypass @15:28:00.32 Toroid.in :default @15:28:00.37 Toroid.seek :clear @15:28:02.04 Cartridge::Sampler::Leaky in FILTERING -- Retained only 17.4psia @15:28:02.40 Retry #1 of 2 @15:28:02.45 Cmd::Error in FILTERING -- No spare cartridges remain @15:28:02.46 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:29:27.75 Session Begun @15:29:44.04 -> Cmd.resume -1 @15:29:44.07 Resuming Thread (suspended since 15:27:53) @15:29:44.09 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:29:48.68 Cartridge pressure fell 33% to 17.1psia @15:29:48.85 Cartridge::Sampler::Leaky in FILTERING -- Retained only 17.1psia @15:29:48.87 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:30:06.58 Cmd::Error in bill -- Not Processing @15:30:06.63 UserAbort in bill -- User Abort!! @15:30:15.55 -> Cartridge.unlock @15:30:15.63 Carousel.in :default @15:30:15.75 Carousel.home.seek 5 @15:30:16.11 Air.in :unlocking @15:30:16.24 Air.seek :clear @15:30:17.61 Lysis1.in :unlocking @15:30:17.75 Plunge.in :unlocking @15:30:17.79 Lysis1.seek :clear @15:30:17.90 Plunge.seek :clear @15:30:36.91 -> slot 5 =>[:dry, :archive_bac] {5 => { Type: :archive_bac, State: :dry}} @15:30:46.10 -> MainThread.exit @15:30:46.18
SamplePump.stop # @15:30:46.29 Session Ended @15:31:03.81PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:31:03.83 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:31:06.41 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:31:06.88 Sea pressure (Abs) tare is 6% of full scale @15:31:12.76 Polling Can every 2:00 @15:31:12.80 Loading /home/esp/esp2/bin/processAll mission @15:31:12.97 Sea pressure (Abs) tare is 6% of full scale @15:31:12.98 Sampling maximum of 445.8ml per cartridge @15:31:12.99 Cartridge group: dry Spares: {spare: 0} @15:31:13.11 Gate.power :analytic1,:ON,limit to 5000mA @15:31:13.13 Gate.power :analytic2,:ON,limit to 5000mA @15:31:13.14 Gate.power :analytic3,:ON,limit to 5000mA @15:31:13.71 Archive.configure :default @15:31:13.76 Archive.hold @15:31:13.81 Archive.configure :default @15:31:13.95 Air.configure :default @15:31:13.99 Air.hold @15:31:14.06 Air.configure :default @15:31:14.12 SPR Module 1, FW Rev. B, FPGA Rev. C @15:31:14.19
Lysis1.configure :default @15:31:14.24 Lysis1.hold @15:31:14.29 Lysis1.configure :default @15:31:14.38 Lysis2.configure :default @15:31:14.43 Lysis2.hold @15:31:14.48 Lysis2.configure :default @15:31:14.60 Plunge.configure :default @15:31:14.65 Plunge.hold @15:31:14.70 Plunge.configure :default @15:31:14.82 SamplePump.configure :default @15:31:14.95 Toroid.configure :default @15:31:15.00 Toroid.hold @15:31:15.05 Toroid.configure :default @15:31:15.17 Carousel.configure :default @15:31:15.22 Carousel.hold @15:31:15.27 Carousel.configure :default @15:31:15.73 Pressure.configure :default @15:31:15.86 ExtraPump.configure :default @15:31:15.96 PinPort.configure :default @15:31:16.01 PinPort.hold @15:31:16.06 PinPort.configure :default @15:31:16.11 All elf controllers running firmware version 4.83 @15:31:16.70 Toroid.seek :clear @15:31:16.76 Carousel.in :default @15:31:16.87 Carousel.home.seek 5 @15:31:17.10 Selecting Cartridge 5 @15:31:17.32 Carousel.seek 5 @15:31:17.49 Lysis1.in :fast @15:31:17.54 Lysis1.seek :locked @15:31:20.13 Plunge.seek :locked @15:31:20.25 Air.in :fast @15:31:20.30 Air.seek :locked @15:31:21.86
Carousel.reconfigure :locked @15:31:21.98 Powering cartridge bus @15:31:24.59 Gate.setIR 1750,5000,0,6 @15:31:24.71 Gate.setIR 0,5000,0,6 @15:31:24.75 Gate.power :cartridge,:OFF @15:31:25.28 Mounted archive Cartridge @15:31:25.53 Preparing to filter cartridge 5 @15:31:25.76 Priming sample loop w/100ml, bypass w/10ml @15:31:25.77 SamplePump.setPosition! 0ml @15:31:25.80 Exhaust.close @15:31:25.87 Bypass.open @15:31:27.34 Intake.open @15:31:28.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:31:30.63 Vacuum tare is 60% of full scale @15:31:30.75 Pressure.in :default @15:31:30.80 Pressure.hold @15:31:31.34 SamplePump.in :prime @15:31:31.40 SamplePump.seek 10ml,59 seconds @15:31:40.12 Pressure.stop @15:31:40.16 SamplePump.setPosition! 0ml @15:31:40.20 Exhaust.open @15:31:41.63 Bypass.close @15:31:43.13 Pressure.in :default @15:31:43.18 Pressure.hold @15:31:43.40 SamplePump.in :prime @15:31:43.46 SamplePump.seek 100ml,5:16 @15:33:12.80 Pressure.stop @15:33:12.86 Bypass.open @15:33:13.56 Intake.close @15:33:14.32 Toroid.in :hold @15:33:14.37 Toroid.seek :crack @15:33:15.89 Toroid.seek :cartridge @15:33:16.98 Toroid.in :default @15:33:17.03 Toroid.seek :clear @15:33:18.98 Pressurizing cartridge to 26psi above 14.8psia @15:33:18.99 SamplePump.setPosition! 0ml @15:33:19.02 Intake.open @15:33:19.69 Bypass.close @15:33:20.38 Exhaust.close @15:33:21.17 Pressure.in :default @15:33:21.22 Pressure.hold @15:33:21.39 SamplePump.in :charge @15:33:21.45 SamplePump.seek 2.3ml,42 seconds @15:33:24.30 Pressure.stop @15:33:24.34 Intake.close @15:33:25.08 Pressurized cartridge with 0.037ml to 26.4psi @15:33:25.09 Isolated @22.7psia (7.9psi) @15:33:25.10 Verifying cartridge holds at least 0.65..0.85 of 22.7psia @15:33:25.12 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:33:29.70 Cartridge pressure fell 21% to 18.0psia @15:33:29.80 Toroid.in :hold @15:33:29.85 Toroid.seek :bypass @15:33:31.48 Toroid.in :default @15:33:31.53 Toroid.seek :clear @15:33:33.20 Cartridge::Sampler::Leaky in FILTERING -- Retained only 18.0psia @15:33:33.21 Retry #1 of 2 @15:33:33.22 Cmd::Error in FILTERING -- No spare cartridges remain @15:33:33.23 Waiting to Resume from Checkpoint "Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi" @15:36:31.68 Session Begun @15:36:54.09 -> BACsampler.examine @15:42:43.70 -> BACsampler.chargeBleed=0.02..0.03 0.02..0.03 @15:42:51.65 -> BACsampler.examine @15:42:59.79 -> Cartridge.unlock @15:42:59.88 Carousel.in :default @15:43:00.00 Carousel.home.seek 5 @15:43:00.36 Air.in :unlocking @15:43:00.49 Air.seek :clear @15:43:01.83 Lysis1.in :unlocking @15:43:01.96 Plunge.in :unlocking @15:43:02.00 Lysis1.seek :clear @15:43:02.11 Plunge.seek :clear @15:43:16.94 -> slot 5 =>[:dry, :archive_bac] {5 => { Type: :archive_bac, State: :dry}} @15:43:31.23 -> processAll '445.8' @15:43:31.34 Sea pressure (Abs) tare is 6% of full scale @15:43:31.35 Sampling maximum of 445.8ml per cartridge @15:43:31.38 Cartridge group: dry Spares: {spare: 0} @15:43:31.46 Selecting Cartridge 5 @15:43:31.71 Carousel.seek 5 @15:43:31.91 Lysis1.in :fast @15:43:31.99 Lysis1.seek :locked @15:43:34.60 Plunge.in :default @15:43:34.70 Plunge.seek :locked @15:43:34.81 Air.in :fast @15:43:34.88 Air.seek :locked @15:43:36.46 Carousel.reconfigure :locked @15:43:36.61 Powering cartridge bus @15:43:39.23 Gate.setIR 1750,5000,0,6 @15:43:39.36 Gate.setIR 0,5000,0,6 @15:43:39.43 Gate.power :cartridge,:OFF @15:43:39.97 Mounted archive Cartridge @15:43:40.26 Preparing to filter cartridge 5 @15:43:40.29 Cmd::Error in MAIN -- Cannot process while in READY state @15:43:40.35
Waiting to Resume from Checkpoint "Selecting Cartridge 5" @15:43:40.54 Bypass.open @15:43:41.35 Toroid.in :hold @15:43:41.42 Toroid.seek :crack @15:43:42.96 Toroid.seek :cartridge @15:43:44.07 Toroid.in :default @15:43:44.14 Toroid.seek :clear @15:43:45.95 Exhaust.open @15:43:46.84 Pressurizing cartridge to 26psi above 14.7psia @15:43:46.85 SamplePump.setPosition! 0ml @15:43:46.91 Intake.open @15:43:47.59 Bypass.close @15:43:48.31 Exhaust.close @15:43:49.55 Pressure.in :default @15:43:49.62 Pressure.hold @15:43:49.82 SamplePump.seek 2.3ml,42 seconds @15:43:52.65 Pressure.stop @15:43:52.72 Intake.close @15:43:53.44 Pressurized cartridge with 0.036ml to 26.3psi @15:43:53.46 Isolated @27.7psia (13.0psi) @15:43:53.48 Verifying cartridge holds at least 0.02..0.03 of 27.7psia @15:43:53.53 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:43:59.11 Cartridge pressure fell 35% to 18.0psia @15:43:59.21 Sampling 445.8ml in 1:25:20 @15:43:59.22 SamplePump.setPosition! 0ml @15:43:59.29 Exhaust.open @15:44:00.09 Intake.open @15:44:00.89 Pressure.in :default @15:44:00.96 Pressure.hold @15:44:01.09 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @15:44:01.24 SamplePump.in :sample @15:44:01.32 SamplePump.seek 445.8ml,37:40 @15:44:03.94 Cartridge 5 filtering 1/446ml at 0.45ml/s with 22.3psi vacuum 2.0m deep (17.7psia) @15:45:03.95 Cartridge 5 filtering 28/446ml at 0.45ml/s with 22.0psi vacuum @15:46:03.94 Cartridge 5 filtering 55/446ml at 0.45ml/s with 24.0psi vacuum @15:47:03.95 Cartridge 5 filtering 83/446ml at 0.46ml/s with 27.0psi vacuum @15:48:03.95 Cartridge 5 filtering 110/446ml at 0.44ml/s with 24.6psi vacuum @15:49:03.94 Cartridge 5 filtering 137/446ml at 0.47ml/s with 23.6psi vacuum 1.7m deep (17.2psia) @15:50:03.94 Cartridge 5 filtering 165/446ml at 0.45ml/s with 26.5psi vacuum @15:51:03.94 Cartridge 5 filtering 192/446ml at 0.46ml/s with 25.6psi vacuum @15:52:03.95 Cartridge 5 filtering 220/446ml at 0.46ml/s with 25.5psi vacuum @15:53:03.94 Cartridge 5 filtering 247/446ml at 0.44ml/s with 24.5psi vacuum @15:54:03.95 Cartridge 5 filtering 275/446ml at 0.48ml/s with 24.3psi vacuum 1.8m deep (17.4psia) @15:55:03.94 Cartridge 5 filtering 303/446ml at 0.45ml/s with 22.4psi vacuum @15:56:03.94 Cartridge 5 filtering 331/446ml at 0.48ml/s with 22.8psi vacuum @15:57:04.35 Cartridge 5 filtering 359/446ml at 0.47ml/s with 25.2psi vacuum @15:58:03.95 Cartridge 5 filtering 387/446ml at 0.46ml/s with 23.0psi vacuum @15:59:03.95 Cartridge 5 filtering 415/446ml at 0.46ml/s with 24.1psi vacuum @16:00:03.95 Cartridge 5 filtering 443/446ml at 0.49ml/s with 27.1psi vacuum @16:00:09.86 Pressure.stop @16:00:09.99 Intake.close @16:00:10.06 Exhaust.close @16:00:10.24 Toroid.in :hold @16:00:10.32 Toroid.seek :bypass @16:00:11.95 Toroid.in :default @16:00:12.02 Toroid.seek :clear @16:00:13.64 Sampled 445.8ml @16:00:14.15 Plunge.seek :reagent @16:00:14.96 Sample loop closed. Ready to Deliver Reagents @16:00:14.98 Archive.seek :sealed @16:00:18.93 Archive.seek :evac @16:00:32.97 delay 1:00 @16:01:33.00 Archive.seek :clear @16:01:50.48 RNAlater soak for 10:00 @16:11:50.51 Archive.seek :tVent @16:11:52.95 delay 1 second @16:11:53.97 Archive.seek :evac @16:12:09.48 delay 10 seconds @16:12:19.50 Sample preserved and evacuated @16:12:19.72 Carousel.in :default @16:12:19.85 Carousel.home.seek 5 @16:12:20.16 Plunge.seek :locked @16:12:21.02 Archive.in :unlocking @16:12:21.16 Archive.seek :clear @16:12:21.22 Air.in :unlocking @16:12:21.39 Air.seek :clear @16:12:21.44 Lysis1.in :unlocking @16:12:21.61 Lysis1.seek :clear @16:12:25.76 Gate.power :motor,:OFF @16:12:25.78 Gate.power :core,:OFF @16:12:25.80 Gate.power :analytic1,:OFF @16:12:25.84 Gate.power :analytic2,:OFF @16:12:25.87 Gate.power :analytic3,:OFF @16:12:25.90 Gate.power :main,:OFF @16:12:25.92 Safely stopped and ready to power off @16:12:26.33 Cartridge::None in bill -- Not enough dry cartridges remain @16:12:33.21 -> slot {[4,2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @16:12:58.64 -> Cartridge.release 4 1 @16:13:13.92 -> MainThread.exit # @16:13:14.01 ThreadError in bill -- killed thread @16:13:14.07 Session Ended @16:13:27.06PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @16:13:27.08 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:13:29.48 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @16:13:29.94 Sea pressure (Abs) tare is 6% of full scale @16:13:35.77 Polling Can every 2:00 @16:13:35.80 Loading /home/esp/esp2/bin/processAll mission @16:13:35.96 Sea pressure (Abs) tare is 6% of full scale @16:13:35.97 Sampling default goal volumes @16:13:35.98 Cartridge group: dry Spares: {spare: 0} @16:13:36.03 Gate.power :main,:ON,limit to 5000mA @16:13:36.04 Gate.power :analytic1,:ON,limit to 5000mA @16:13:36.05 Gate.power :analytic2,:ON,limit to 5000mA @16:13:36.06 Gate.power :analytic3,:ON,limit to 5000mA @16:13:36.16 Powering core bus @16:13:38.46 SPR Module 1, FW Rev. B, FPGA Rev. C @16:13:38.85
Archive.configure :default @16:13:38.92 Air.configure :default @16:13:38.97 Lysis1.configure :default @16:13:39.02 Lysis2.configure :default @16:13:39.09 Plunge.configure :default @16:13:39.17 SamplePump.configure :default @16:13:39.24 Toroid.configure :default @16:13:39.31 Carousel.configure :default @16:13:39.38 Pressure.configure :default @16:13:39.46 ExtraPump.configure :default @16:13:39.51 PinPort.configure :default @16:13:39.56 All elf controllers running firmware version 4.83 @16:13:39.58 Gate.power :motor,:ON,limit to 5000mA @16:13:39.93 Lysis2.home.jog 0 @16:13:40.01 Archive.home.jog 0 @16:13:40.10 Lysis1.home.jog 0 @16:13:40.15 Air.home.jog 0 @16:13:40.50 Carousel.home.jog 0 @16:13:40.53 Toroid.home.jog 0 @16:13:40.59 PinPort.home.jog 0 @16:13:40.67 Plunge.home.jog 0 @16:13:41.43 Lysis1.in :homing @16:13:41.51 Air.in :homing @16:13:41.55 Archive.in :homing @16:13:41.58 Lysis2.in :homing @16:13:41.71 Lysis1.jog 151 @16:13:41.76 Lysis2.jog 1394 @16:13:41.78 Air.jog 1380 @16:13:41.81 Archive.jog 1385 @16:13:42.03 PinPort.in :homing @16:13:42.06 Plunge.in :homing @16:13:42.09 Toroid.in :homing @16:13:42.17 PinPort.jog -106 @16:13:42.23 Toroid.jog 618 @16:13:42.25 Plunge.jog 1564 @16:13:42.38 Lysis1.setRawPosition! 50 @16:13:42.42 Lysis1.seek :home @16:13:42.47 PinPort.setRawPosition! 0 @16:13:42.53 PinPort.seek :home @16:13:42.59 PinPort.in :default @16:13:42.68 Lysis1.in :default @16:13:42.98 Air.setRawPosition! 50 @16:13:43.05 Lysis2.setRawPosition! 50 @16:13:43.07 Air.seek :home @16:13:43.15 Lysis2.seek :home @16:13:43.21 Archive.setRawPosition! 50 @16:13:43.25 Archive.seek :home @16:13:43.35 Air.in :default @16:13:43.43 Lysis2.in :default @16:13:43.51 Archive.in :default @16:13:43.59 Plunge.setRawPosition! 50 @16:13:43.64 Plunge.seek :home @16:13:43.90 Plunge.in :default @16:13:44.37 Toroid.setRawPosition! 0 @16:13:44.41 Toroid.seek :home @16:13:44.47 Toroid.in :default @16:13:44.54
Toroid.seek :clear @16:13:46.13 Carousel.in :default @16:13:46.23 Carousel.home.seek 5 @16:13:46.47 Selecting Cartridge 4 @16:13:46.70 Carousel.seek 4 @16:13:47.16 Lysis1.in :fast @16:13:47.21 Lysis1.seek :locked @16:13:49.79 Plunge.seek :locked @16:13:49.91 Air.in :fast @16:13:49.96 Air.seek :locked @16:13:51.53
Carousel.reconfigure :locked @16:13:51.66 Powering cartridge bus @16:13:58.26 Gate.power :cartridge,:OFF @16:13:58.28 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @16:13:58.29 Retry #1 of 2 @16:13:58.32 Cmd::Error in MAIN -- No spare cartridges remain @16:13:58.33 Waiting to Resume from Checkpoint "Selecting Cartridge 4" @16:14:44.05 Session Begun @16:14:47.49 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :dry}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @16:15:19.96 -> MainThread.exit # @16:15:20.47 ThreadError in bill -- killed thread @16:15:20.52 Session Ended @16:15:31.34PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @16:15:31.36 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:15:33.82 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @16:15:34.27 Sea pressure (Abs) tare is 6% of full scale @16:15:40.17 Polling Can every 2:00 @16:15:40.20 Loading /home/esp/esp2/bin/processAll mission @16:15:40.36 Sea pressure (Abs) tare is 6% of full scale @16:15:40.37 Sampling default goal volumes @16:15:40.38 Cartridge group: dry Spares: {spare: 0} @16:15:40.51 Gate.power :analytic1,:ON,limit to 5000mA @16:15:40.52 Gate.power :analytic2,:ON,limit to 5000mA @16:15:40.53 Gate.power :analytic3,:ON,limit to 5000mA @16:15:41.11 Archive.configure :default @16:15:41.23 Air.configure :default @16:15:41.28 Air.hold @16:15:41.34 Air.configure :default @16:15:41.40 SPR Module 1, FW Rev. B, FPGA Rev. C @16:15:41.48
Lysis1.configure :default @16:15:41.53 Lysis1.hold @16:15:41.58 Lysis1.configure :default @16:15:41.68 Lysis2.configure :default @16:15:41.81 Plunge.configure :default @16:15:41.86 Plunge.hold @16:15:41.91 Plunge.configure :default @16:15:42.03 SamplePump.configure :default @16:15:42.15 Toroid.configure :default @16:15:42.20 Toroid.hold @16:15:42.25 Toroid.configure :default @16:15:42.37 Carousel.configure :default @16:15:42.42 Carousel.hold @16:15:42.47 Carousel.configure :default @16:15:42.59 Pressure.configure :default @16:15:42.71 ExtraPump.configure :default @16:15:43.15 PinPort.configure :default @16:15:43.20 All elf controllers running firmware version 4.83 @16:15:43.76 Toroid.seek :clear @16:15:43.82 Carousel.in :default @16:15:43.92 Carousel.home.seek 4 @16:15:44.27 Carousel.reconfigure :locked @16:15:44.39 Powering cartridge bus @16:15:47.00 Gate.setIR 1750,5000,0,6 @16:15:47.12 Gate.setIR 0,5000,0,6 @16:15:47.17 PuckHeater.configure :default @16:15:47.21 Gate.power :heat,:ON,limit to 5000mA @16:15:49.87 Mounted lysis Cartridge board#Q05076 @16:15:49.90 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:15:49.93 Selecting Cartridge 4 @16:15:50.42 Preparing to filter cartridge 4 @16:15:50.65 Priming sample loop w/100ml, bypass w/10ml @16:15:50.66 SamplePump.setPosition! 0ml @16:15:50.69 Exhaust.close @16:15:50.75 Bypass.open @16:15:52.24 Intake.open @16:15:52.91 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:15:55.47 Vacuum tare is 60% of full scale @16:15:55.59 Pressure.in :default @16:15:55.64 Pressure.hold @16:15:55.81 SamplePump.in :prime @16:15:55.87 SamplePump.seek 10ml,59 seconds @16:16:04.60 Pressure.stop @16:16:04.64 SamplePump.setPosition! 0ml @16:16:04.68 Exhaust.open @16:16:06.14 Bypass.close @16:16:08.04 Pressure.in :default @16:16:08.09 Pressure.hold @16:16:08.32 SamplePump.in :prime @16:16:08.37 SamplePump.seek 100ml,5:16 @16:17:35.87 Pressure.stop @16:17:35.93 Bypass.open @16:17:36.66 Intake.close @16:17:37.37 Toroid.in :hold @16:17:37.42 Toroid.seek :crack @16:17:38.94 Toroid.seek :cartridge @16:17:40.03 Toroid.in :default @16:17:40.08 Toroid.seek :clear @16:17:42.05 Pressurizing cartridge to 26psi above 14.8psia @16:17:42.07 SamplePump.setPosition! 0ml @16:17:42.10 Intake.open @16:17:42.73 Bypass.close @16:17:43.44 Exhaust.close @16:17:44.25 Pressure.in :default @16:17:44.30 Pressure.hold @16:17:44.47 SamplePump.in :charge @16:17:44.52 SamplePump.seek 2.3ml,42 seconds @16:17:56.96 Pressure.stop @16:17:57.00 Intake.close @16:17:57.74 Pressurized cartridge with 0.735ml to 26.0psi @16:17:57.75 Isolated @39.4psia (24.6psi) @16:17:57.76 Verifying cartridge holds at least 0.65..0.85 of 39.4psia @16:17:57.78 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:18:02.42 Sampling 1000.0ml in 1:25:20 @16:18:02.43 SamplePump.setPosition! 0ml @16:18:02.47 Exhaust.open @16:18:03.22 Intake.open @16:18:04.02 Pressure.in :default @16:18:04.07 Pressure.hold @16:18:04.18 Sea 2.5m deep (18.4psia) @16:18:04.19 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @16:18:04.31 SamplePump.in :sample @16:18:04.37 SamplePump.seek 1000ml,1:23:51 @16:18:07.07 Cartridge 4 filtering 1/1000ml at 0.49ml/s with 25.0psi vacuum @16:19:07.07 Cartridge 4 filtering 31/1000ml at 0.49ml/s with 24.3psi vacuum @16:20:07.07 Cartridge 4 filtering 61/1000ml at 0.50ml/s with 24.6psi vacuum @16:21:07.07 Cartridge 4 filtering 91/1000ml at 0.50ml/s with 25.0psi vacuum @16:22:07.08 Cartridge 4 filtering 121/1000ml at 0.51ml/s with 23.8psi vacuum @16:23:07.08 Cartridge 4 filtering 151/1000ml at 0.51ml/s with 24.8psi vacuum @16:24:07.08 Cartridge 4 filtering 181/1000ml at 0.52ml/s with 24.9psi vacuum @16:25:07.07 Cartridge 4 filtering 211/1000ml at 0.51ml/s with 25.1psi vacuum @16:26:07.08 Cartridge 4 filtering 241/1000ml at 0.49ml/s with 23.2psi vacuum @16:27:07.07 Cartridge 4 filtering 272/1000ml at 0.50ml/s with 24.8psi vacuum @16:28:07.07 Cartridge 4 filtering 302/1000ml at 0.51ml/s with 25.7psi vacuum @16:29:07.07 Cartridge 4 filtering 333/1000ml at 0.51ml/s with 25.1psi vacuum @16:30:07.08 Cartridge 4 filtering 363/1000ml at 0.51ml/s with 24.1psi vacuum @16:31:07.07 Cartridge 4 filtering 394/1000ml at 0.52ml/s with 23.5psi vacuum @16:32:07.07 Cartridge 4 filtering 424/1000ml at 0.51ml/s with 23.2psi vacuum @16:33:07.07 Cartridge 4 filtering 455/1000ml at 0.51ml/s with 25.1psi vacuum @16:34:07.07 Cartridge 4 filtering 485/1000ml at 0.50ml/s with 23.1psi vacuum @16:35:07.07 Cartridge 4 filtering 516/1000ml at 0.51ml/s with 22.9psi vacuum @16:36:07.07 Cartridge 4 filtering 547/1000ml at 0.51ml/s with 25.8psi vacuum @16:37:07.07 Cartridge 4 filtering 578/1000ml at 0.53ml/s with 23.7psi vacuum @16:38:07.07 Cartridge 4 filtering 609/1000ml at 0.53ml/s with 24.8psi vacuum @16:39:07.07 Cartridge 4 filtering 640/1000ml at 0.53ml/s with 25.1psi vacuum @16:40:07.07 Cartridge 4 filtering 671/1000ml at 0.52ml/s with 24.9psi vacuum @16:41:07.07 Cartridge 4 filtering 702/1000ml at 0.52ml/s with 24.8psi vacuum @16:42:07.07 Cartridge 4 filtering 733/1000ml at 0.53ml/s with 24.0psi vacuum 1.6m deep (17.1psia) @16:43:07.07 Cartridge 4 filtering 764/1000ml at 0.52ml/s with 23.2psi vacuum @16:44:07.07 Cartridge 4 filtering 796/1000ml at 0.53ml/s with 23.6psi vacuum @16:45:07.07 Cartridge 4 filtering 827/1000ml at 0.53ml/s with 25.0psi vacuum @16:46:07.07 Cartridge 4 filtering 858/1000ml at 0.52ml/s with 22.9psi vacuum @16:47:07.07 Cartridge 4 filtering 890/1000ml at 0.54ml/s with 24.0psi vacuum @16:48:07.07 Cartridge 4 filtering 922/1000ml at 0.54ml/s with 23.5psi vacuum @16:49:07.07 Cartridge 4 filtering 955/1000ml at 0.54ml/s with 24.9psi vacuum @16:50:07.07 Cartridge 4 filtering 988/1000ml at 0.55ml/s with 23.7psi vacuum @16:50:29.22 Pressure.stop @16:50:29.32 Intake.close @16:50:29.37 Exhaust.close @16:50:29.54 Toroid.in :hold @16:50:30.12 Toroid.seek :bypass @16:50:31.75 Toroid.in :default @16:50:31.80 Toroid.seek :clear @16:50:33.40 Sampled 1000.0ml @16:50:34.92 SPR.command "read_sequences" @16:50:35.38 SPR.command "stats_on" @16:50:35.51 SPR.command "start 0 1" @16:50:36.23 SPR.start /var/log/esp/spr23apr2616h.spr.bz2 @16:50:38.23 Handoff.select :waste @16:50:44.92 Syringe tare is 17% of full scale @16:50:44.93 PinPort.in :locking @16:50:44.98 PinPort.seek :locked @16:50:45.45 Archive.in :fast @16:50:45.49 Lysis2.in :fast @16:50:45.54 Lysis1.in :fast @16:50:45.66 Lysis1.seek 1350uL @16:50:45.69 Lysis2.seek :full @16:50:45.71 Archive.seek :full @16:50:47.67 Plunge.seek :reagent @16:50:48.53 Evacuating puck with filter bubble point of 50psi @16:50:48.55 PinPort.seek :locked @16:50:48.61 Handoff.select :waste @16:50:55.20 Air syringe pumping up to 1 strokes to reach 8psi @16:50:55.21 Air stroke #1 of 1 @16:50:55.27 Air.in :pressurize @16:50:55.33 Air.seek :pressurized @16:51:01.04 1.3psi after pressurize @16:51:01.05 Air.in :compress @16:51:01.10 Air.seek :evac @16:51:04.09 1.0psi after compress @16:51:14.67 -0.1psi after compress (stable) @16:51:14.70 Air.in :default @16:51:14.75 Air.hold @16:51:14.92 at -0.1psi Opened to waste @16:51:14.93 Handoff.select :blocked @16:51:15.05 Archive syringe pumping up to 8 strokes to reach 50psi @16:51:15.06 Archive stroke #1 of 8 @16:51:15.13 Archive.in :pressurize @16:51:15.18 Archive.seek :pressurized @16:51:27.67 -0.1psi after pressurize @16:51:49.25 15.5psi after pressurize (stable) @16:51:49.26 Archive.in :compress @16:51:49.31 Archive.seek :empty @16:52:03.93 16.2psi after compress @16:52:14.51 16.3psi after compress (stable) @16:52:14.52 Archive.in :gulp @16:52:14.57 Archive.seek :topVent @16:52:20.80 16.4psi after gulp @16:52:26.37 16.4psi after gulp (stable) @16:52:26.38 Archive stroke #2 of 8 @16:52:26.45 Archive.in :pressurize @16:52:26.50 Archive.seek :pressurized @16:52:39.01 16.1psi after pressurize @16:52:44.95 16.0psi after pressurize (stable) @16:52:44.96 Archive.in :compress @16:52:45.01 Archive.seek :empty @16:52:59.62 16.6psi after compress @16:53:10.20 16.5psi after compress (stable) @16:53:10.22 Archive.in :gulp @16:53:10.27 Archive.seek :topVent @16:53:16.49 16.8psi after gulp @16:53:22.07 16.8psi after gulp (stable) @16:53:22.08 Archive stroke #3 of 8 @16:53:22.14 Archive.in :pressurize @16:53:22.19 Archive.seek :pressurized @16:53:34.70 16.5psi after pressurize @16:53:40.28 16.4psi after pressurize (stable) @16:53:40.30 Archive.in :compress @16:53:40.39 Archive.seek :empty @16:53:55.00 17.1psi after compress @16:54:17.09 23.2psi after compress (stable) @16:54:17.10 Archive.in :gulp @16:54:17.15 Archive.seek :topVent @16:54:23.40 25.4psi after gulp @16:54:34.47 27.3psi after gulp (stable) @16:54:34.48 Archive stroke #4 of 8 @16:54:34.55 Archive.in :pressurize @16:54:34.60 Archive.seek :pressurized @16:54:47.08 27.7psi after pressurize @16:54:52.66 27.3psi after pressurize (stable) @16:54:52.67 Archive.in :compress @16:54:52.72 Archive.seek :empty @16:55:07.33 27.6psi after compress @16:56:06.43 13.4psi after compress (stable) @16:56:06.44 Archive.in :gulp @16:56:06.49 Archive.seek :topVent @16:56:12.72 15.3psi after gulp @16:56:19.30 14.2psi after gulp (stable) @16:56:19.31 Archive stroke #5 of 8 @16:56:19.37 Archive.in :pressurize @16:56:19.43 Archive.seek :pressurized @16:56:32.22 12.3psi after pressurize @16:57:01.30 10.3psi after pressurize (stable) @16:57:01.31 Archive.in :compress @16:57:01.36 Archive.seek :empty @16:57:15.98 13.9psi after compress @16:57:47.07 10.4psi after compress (stable) @16:57:47.09 Archive.in :gulp @16:57:47.13 Archive.seek :topVent @16:57:53.39 11.9psi after gulp @16:57:58.97 12.0psi after gulp (stable) @16:57:58.98 Archive stroke #6 of 8 @16:57:59.05 Archive.in :pressurize @16:57:59.10 Archive.seek :pressurized @16:58:11.58 10.3psi after pressurize @16:58:17.15 9.9psi after pressurize (stable) @16:58:17.17 Archive.in :compress @16:58:17.22 Archive.seek :empty @16:58:31.83 14.0psi after compress @16:59:07.91 9.6psi after compress (stable) @16:59:07.92 Archive.in :gulp @16:59:07.97 Archive.seek :topVent @16:59:14.23 10.5psi after gulp @16:59:19.81 10.7psi after gulp (stable) @16:59:19.82 Archive stroke #7 of 8 @16:59:19.92 Archive.in :pressurize @16:59:19.97 Archive.seek :pressurized @16:59:32.44 9.4psi after pressurize @16:59:38.02 9.1psi after pressurize (stable) @16:59:38.04 Archive.in :compress @16:59:38.09 Archive.seek :empty @16:59:52.69 13.5psi after compress @17:00:29.76 8.9psi after compress (stable) @17:00:29.78 Archive.in :gulp @17:00:29.83 Archive.seek :topVent @17:00:36.09 9.9psi after gulp @17:00:41.66 10.2psi after gulp (stable) @17:00:41.67 Archive stroke #8 of 8 @17:00:41.74 Archive.in :pressurize @17:00:41.80 Archive.seek :pressurized @17:00:54.27 8.9psi after pressurize @17:00:59.85 8.6psi after pressurize (stable) @17:00:59.86 Archive.in :compress @17:00:59.91 Archive.seek :empty @17:01:14.52 13.3psi after compress @17:01:53.60 8.4psi after compress (stable) @17:01:53.61 Archive.in :default @17:01:53.66 Archive.hold @17:01:53.77 Handoff.select :blocked @17:01:53.80 Underpressure in PROCESSING -- Reached only 8.3 of 50.0psi after pumping 13840uL @17:01:53.81 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @17:02:51.61 Session Begun @17:02:56.79 -> progress >>> Thread[:MAIN] is sleeping <<< @16:15:40 Cartridge group: dry Spares: {spare: 0} @16:15:49 Selecting Cartridge 4 >>> Thread[:PROCESSING] is suspended <<< @16:50:48 Evacuating puck with filter bubble point of 50psi @16:50:55 Air syringe pumping up to 1 strokes to reach 8psi @16:51:14 Archive syringe pumping up to 8 strokes to reach 50psi @17:03:25.33 -> Cmd.process @17:03:25.35 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:03:49.41 -> Cmd.resume @17:03:49.47 Resuming Thread (suspended since 16:51:14) @17:03:49.50 Archive syringe pumping up to 8 strokes to reach 50psi @17:03:49.52 Archive.hold @17:03:49.68 Handoff.select :blocked @17:03:49.73 Underpressure in PROCESSING -- Reached only 6.5 of 50.0psi after pumping 13840uL @17:03:49.75 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @17:04:33.65 Cmd::Error in bill -- Not Processing @17:04:33.69 UserAbort in bill -- User Abort!! @17:04:49.09 -> progress >>> Thread[:MAIN] is sleeping <<< @16:15:40 Cartridge group: dry Spares: {spare: 0} @16:15:49 Selecting Cartridge 4 >>> Thread[:PROCESSING] is suspended <<< @16:50:48 Evacuating puck with filter bubble point of 50psi @16:50:55 Air syringe pumping up to 1 strokes to reach 8psi @16:51:14 Archive syringe pumping up to 8 strokes to reach 50psi >>> Thread["bill"] is running <<< @17:04:32 User Abort!! @17:05:04.18 -> checkpoint :checkpoint @17:05:10.14 -> checkpoint -2 @17:05:10.16 NoMethodError in bill -- undefined method `checkpoint' for CmdServer::Client:Class @17:05:17.95 -> progress -3 @17:05:17.97 NoMethodError in bill -- undefined method `moribund?' for -3:Fixnum @17:06:05.55 -> Cmd.resume @17:06:05.59 Resuming Thread (suspended since 16:51:14) @17:06:05.62 Archive syringe pumping up to 8 strokes to reach 50psi @17:06:05.65 Archive.hold @17:06:05.82 Handoff.select :blocked @17:06:05.87 Underpressure in PROCESSING -- Reached only 6.1 of 50.0psi after pumping 13840uL @17:06:05.89 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @17:06:18.10 Cmd::Error in bill -- Not Processing @17:06:18.14 UserAbort in bill -- User Abort!! @17:06:21.58 -> Handoff Handoff Valve::Manifold selects blocked @17:06:26.21 -> PP Handoff Pin Port (PP) cartridge @17:06:33.12 -> PV Plunge Valve (PV) at reagentLG @17:06:44.85 -> ArS Archive Syringe (ArS) empty @17:06:50.59 -> AirS Air Syringe (AirS) at evac @17:08:18.21 -> Cmd.resume -6 @17:08:18.23 NoMethodError in bill -- undefined method `riase' for Cmd:Module @17:08:41.82 -> Cmd.resume -1 @17:08:41.86 Resuming Thread (suspended since 16:51:14) @17:08:41.88 Archive syringe pumping up to 8 strokes to reach 50psi @17:08:41.90 Archive.hold @17:08:42.05 Handoff.select :blocked @17:08:42.10 Underpressure in PROCESSING -- Reached only 5.4 of 50.0psi after pumping 13840uL @17:08:42.12 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @17:08:55.99 Cmd::Error in bill -- Not Processing @17:08:56.04 UserAbort in bill -- User Abort!! @17:09:02.95 -> Cmd.startResume @17:09:02.98 Resuming Thread (suspended since 16:51:14) # @17:09:03.03 Archive syringe pumping up to 8 strokes to reach 50psi @17:09:03.06 Archive.hold @17:09:03.22 Handoff.select :blocked @17:09:03.27 Underpressure in PROCESSING -- Reached only 5.4 of 50.0psi after pumping 13840uL @17:09:03.29 Waiting to Resume from Checkpoint "Archive syringe pumping up to 8 strokes to reach 50psi" @17:09:09.72 -> showlog @17:09:29.36 -> Cmd.process @17:09:29.38 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:09:43.71 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:10:35.37 -> Cmd {loadedCartridge: 4, state: :PROCESS_FAILED, type: :daSPR_phyto} @17:11:39.44 -> slot 4 =>:filtered @17:11:39.47 Cartridge::InvalidTypeOrState in bill -- :filtered is neither type nor state @17:12:10.27 -> slot.examine {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:12:36.65 -> Cartridge.filtered 4 @17:12:36.68 Cartridge::TypeUnknown in bill -- 4 is not aRNAl_bac, aRNAl_phyto, aRNAl_zoo, archiveHiBiomass_bac, archiveNull_bac, archive_bac, archive_phyto, archive_zoo, daSPR_bac, daSPR_phyto, daSPR_phyto9, daSPR_zoo, lyseHold_bac, lyseHold_phyto, lyseHold_zoo, lyseNull_bac, microSPR_bac, microSPR_phyto, microSPR_zoo or unknown @17:13:40.85 -> Cmd.examine {loadedCartridge: 4, state: :PROCESS_FAILED, type: :daSPR_phyto} @17:14:19.84 -> Cmd.process @17:14:19.87 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:14:54.70 -> slot 4 =>:filter @17:14:54.73 Cartridge::InvalidTypeOrState in bill -- :filter is neither type nor state @17:15:04.08 -> slot 4 =>:filtered @17:15:04.11 Cartridge::InvalidTypeOrState in bill -- :filtered is neither type nor state @17:15:12.58 -> slot 4 =>:dry {4 => { Type: :daSPR_phyto, State: :dry, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}} @17:15:23.36 -> Cmd.process @17:15:23.38 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:15:53.15 -> slot 4 =>:PROCESS_FAILED @17:15:53.18 Cartridge::InvalidTypeOrState in bill -- :PROCESS_FAILED is neither type nor state @17:16:18.55 -> slot 4=>:lysing {4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}} @17:16:25.11 -> Cmd.process @17:16:25.14 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:17:05.71 -> Cartridge.unlock @17:17:05.72 Gate.power :heat,:OFF @17:17:05.75 Gate.power :cartridge,:OFF @17:17:05.86 PinPort.in :unlocking @17:17:05.94 PinPort.seek :clear @17:17:06.42 Carousel.in :default @17:17:06.54 Carousel.home.seek 4 @17:17:06.87 Archive.in :unlocking @17:17:07.02 Archive.seek :clear @17:17:07.46 Air.in :unlocking @17:17:07.61 Air.seek :clear @17:17:07.70 Lysis2.in :unlocking @17:17:07.85 Lysis2.seek :clear @17:17:11.90 Lysis1.in :unlocking @17:17:12.03 Lysis1.seek :clear @17:17:12.09 Plunge.in :unlocking @17:17:12.19 Plunge.seek :clear @17:17:17.68 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:17:30.92 -> Cartridge.load @17:17:30.95 NoMethodError in bill -- undefined method `load' for Cartridge @17:17:35.18 -> Cartridge.lock @17:17:35.33 Lysis1.in :fast @17:17:35.40 Lysis1.seek :locked @17:17:38.02 Plunge.in :default @17:17:38.14 Plunge.seek :locked @17:17:38.23 Air.in :fast @17:17:38.30 Air.seek :locked @17:17:39.89 Carousel.reconfigure :locked @17:17:40.05 Powering cartridge bus @17:17:42.67 Gate.setIR 1750,5000,0,6 @17:17:42.79 Gate.setIR 0,5000,0,6 @17:17:42.86 PuckHeater.configure :default @17:17:42.93 Gate.power :heat,:ON,limit to 5000mA @17:17:43.57 Mounted lysis Cartridge board#Q05076 @17:17:43.61 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @17:17:48.69 -> Cmd.process @17:17:48.72 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:18:11.53 -> Cmd.abortProcessing @17:18:11.55 Cmd::Error in bill -- Not Processing @17:19:13.30 Ended log spr23apr2616h.spr.bz2 @17:19:13.31 SPR.command "stats_off" @17:19:25.79 -> progress >>> Thread[:MAIN] is sleeping <<< @16:15:40 Cartridge group: dry Spares: {spare: 0} @16:15:49 Selecting Cartridge 4 >>> Thread[:PROCESSING] is suspended <<< @16:50:48 Evacuating puck with filter bubble point of 50psi @16:50:55 Air syringe pumping up to 1 strokes to reach 8psi @16:51:14 Archive syringe pumping up to 8 strokes to reach 50psi >>> Thread["bill"] is running <<< @17:04:32 User Abort!! @17:06:17 User Abort!! @17:08:55 User Abort!! @17:19:57.36 -> SPR.start @17:19:57.41 SPR.command "stats_on" # @17:19:58.37 SPR.start /var/log/esp/spr23apr2617h.spr.bz2 @17:20:04.07 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:20:22.08 -> Cmd.process @17:20:22.10 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:20:41.94 -> SPR.stop @17:20:42.21 Ended log spr23apr2617h.spr.bz2 @17:20:42.23 SPR.command "stats_off" @17:20:47.62 -> Cartridge.unlock @17:20:47.64 Gate.power :heat,:OFF @17:20:47.67 Gate.power :cartridge,:OFF @17:20:47.77 Carousel.in :default @17:20:47.90 Carousel.home.seek 4 @17:20:48.27 Air.in :unlocking @17:20:48.41 Air.seek :clear @17:20:49.80 Lysis1.in :unlocking @17:20:49.93 Lysis1.seek :clear @17:20:49.98 Plunge.in :unlocking @17:20:50.43 Plunge.seek :clear @17:21:01.11 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:21:14.22 -> Cmd.process @17:21:14.24 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:21:23.27 -> Cartridge.lock @17:21:23.40 Lysis1.in :fast @17:21:23.47 Lysis1.seek :locked @17:21:26.07 Plunge.in :default @17:21:26.18 Plunge.seek :locked @17:21:26.27 Air.in :fast @17:21:26.35 Air.seek :locked @17:21:27.92 Carousel.reconfigure :locked @17:21:28.06 Powering cartridge bus @17:21:30.68 Gate.setIR 1750,5000,0,6 @17:21:30.80 Gate.setIR 0,5000,0,6 @17:21:30.87 PuckHeater.configure :default @17:21:30.94 Gate.power :heat,:ON,limit to 5000mA @17:21:31.57 Mounted lysis Cartridge board#Q05076 @17:21:31.60 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @17:21:38.88 -> SPR.start @17:21:38.93 SPR.command "stats_on" # @17:21:40.43 SPR.start /var/log/esp/spr23apr2617h#2.spr.bz2 @17:21:46.62 -> Cmd.process @17:21:46.65 Cmd::Error in bill -- Cannot process while in PROCESS_FAILED state @17:27:43.95 -> slot {[2,1] => { Type: :daSPR_phyto, State: :reserved, was: :dry}, 3 => { Type: :archive_bac, State: :reserved, was: :dry}, 4 => { Type: :daSPR_phyto, State: :lysing, filtered: 1000.0, spr: spr23apr2616h.spr.bz2}, 5 => { Type: :archive_bac, State: :processed, filtered: 445.8}, [42,40,38,36,30,24,22,18,16,14,12,10,6] => { Type: :archive_bac, State: :processed, filtered: 1000}, 7 => { Type: :daSPR_phyto, State: :processed, filtered: 903.4, lysed: 825, spr: "spr23apr2516h.spr.bz2"}, 8 => { Type: :archive_bac, State: :processed, filtered: 860.3}, 9 => { Type: :daSPR_phyto, State: :processed, filtered: 729.6, lysed: 825, spr: "spr23apr2514h.spr.bz2"}, 11 => { Type: :daSPR_phyto, State: :processed, filtered: 883.9, lysed: 825, spr: "spr23apr2409h.spr.bz2"}, 13 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2319h.spr.bz2"}, 15 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2316h.spr.bz2"}, 17 => { Type: :daSPR_phyto, State: :processed, filtered: 902, lysed: 825, spr: "spr23apr2313h.spr.bz2"}, 19 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2212h.spr.bz2"}, 20 => { Type: :archive_bac, State: :processed, filtered: 801.2}, 21 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2109h.spr.bz2"}, 23 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2021h.spr.bz2"}, 25 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2016h.spr.bz2"}, 26 => { Type: :archive_bac, State: :processed, filtered: 544.7}, 27 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr2009h.spr.bz2"}, 28 => { Type: :archive_bac, State: :processed, filtered: 970.7}, 29 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1922h.spr.bz2"}, 31 => { Type: :daSPR_phyto, State: :processed, filtered: 672.1, lysed: 825, spr: "spr23apr1913h.spr.bz2"}, 32 => { Type: :archive_bac, State: :processed, filtered: 711.5}, 33 => { Type: :daSPR_phyto, State: :leaky}, 34 => { Type: :archive_bac, State: :preserving, filtered: 167.4}, 35 => { Type: :daSPR_phyto, State: :processed, filtered: 814.5, lysed: 825, spr: "spr23apr1908h.spr.bz2"}, 37 => { Type: :daSPR_phyto, State: :processed, filtered: 953.9, lysed: 825, spr: "spr23apr1901h.spr.bz2"}, 39 => { Type: :daSPR_phyto, State: :processed, filtered: 593.1, lysed: 825, spr: "spr23apr1818h.spr.bz2"}, 41 => { Type: :daSPR_phyto, State: :processed, filtered: 785.8, lysed: 825, spr: "spr23apr1717h.spr.bz2"}, 43 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1710h.spr.bz2"}, 44 => { Type: :archive_bac, State: :processed, filtered: 599.4}, 45 => { Type: :archiveNull_bac, State: :reserved, was: :dry}, 46 => { Type: :archive_bac, State: :processed, filtered: 319.3}, [58,47] => { Type: :unknown, State: :processed, was: :processed}, 48 => { Type: :daSPR_phyto, State: :processed, filtered: 909.3, lysed: 825, spr: "spr23apr1616h.spr.bz2"}, 49 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1610h.spr.bz2"}, 50 => { Type: :daSPR_phyto, State: :processed, filtered: 1000, lysed: 825, spr: "spr23apr1520h.spr.bz2"}, 51 => { Type: :daSPR_phyto, State: :processed, filtered: 924.9, lysed: 825, spr: "spr23apr1508h.spr.bz2"}, 52 => { Type: :daSPR_phyto, State: :processed, filtered: 317.3, lysed: 825, spr: "spr23apr1419h.spr.bz2"}, 53 => { Type: :daSPR_phyto, State: :reserved, lysed: 1000, lysed2: 1000, was: :standard}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2415h.spr.bz2"}, 55 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23apr2413h.spr.bz2"}, 56 => { Type: :daSPR_phyto, State: :processed, filtered: 250, lysed: 825, spr: "spr23apr1413h.spr.bz2"}, 57 => { Type: :daSPR_phyto, State: :processed, filtered: 604.6, lysed: 825, spr: "spr23apr1409h.spr.bz2"}, 59 => { Type: :lyseHold_phyto, State: :processed, was: :processed}, 60 => { Type: :daSPR_phyto, State: :processed, filtered: 330.3, lysed: 825, spr: "spr23apr1318h.spr.bz2"}} @17:28:05.84 -> Cartridge.release 2 1 @17:28:19.11 -> Cartridge.unlock @17:28:19.12 Gate.power :heat,:OFF @17:28:19.16 Gate.power :cartridge,:OFF @17:28:19.26 Carousel.in :default @17:28:19.40 Carousel.home.seek 4 @17:28:19.77 Air.in :unlocking @17:28:19.90 Air.seek :clear @17:28:21.26 Lysis1.in :unlocking @17:28:21.70 Lysis1.seek :clear @17:28:21.80 Plunge.in :unlocking @17:28:21.92 Plunge.seek :clear @17:28:34.81 -> MainThrea.exit @17:28:34.83 NameError in bill -- uninitialized constant MainThrea @17:28:40.30 -> MainThread.exit # @17:28:42.35 Ended log spr23apr2617h#2.spr.bz2 @17:28:42.36
SPR.command "stats_off" @17:28:42.52 Session Ended @17:28:57.87PDT26-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:28:57.89 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:29:00.31 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @17:29:00.79 Sea pressure (Abs) tare is 6% of full scale @17:29:06.66 Polling Can every 2:00 @17:29:06.70 Loading /home/esp/esp2/bin/processAll mission @17:29:06.85 Sea pressure (Abs) tare is 6% of full scale @17:29:06.86 Sampling default goal volumes @17:29:06.87 Cartridge group: dry Spares: {spare: 0} @17:29:07.00 Gate.power :analytic1,:ON,limit to 5000mA @17:29:07.01 Gate.power :analytic2,:ON,limit to 5000mA @17:29:07.02 Gate.power :analytic3,:ON,limit to 5000mA @17:29:07.60 Archive.configure :default @17:29:07.65 Archive.hold @17:29:07.70 Archive.configure :default @17:29:07.82 Air.configure :default @17:29:07.87 Air.hold @17:29:07.92 Air.configure :default @17:29:08.04 SPR Module 1, FW Rev. B, FPGA Rev. C @17:29:08.07
Lysis1.configure :default @17:29:08.12 Lysis1.hold @17:29:08.17 Lysis1.configure :default @17:29:08.27 Lysis2.configure :default @17:29:08.32 Lysis2.hold @17:29:08.37 Lysis2.configure :default @17:29:08.49 Plunge.configure :default @17:29:08.54 Plunge.hold @17:29:08.59 Plunge.configure :default @17:29:08.71 SamplePump.configure :default @17:29:08.83 Toroid.configure :default @17:29:08.88 Toroid.hold @17:29:08.94 Toroid.configure :default @17:29:09.06 Carousel.configure :default @17:29:09.11 Carousel.hold @17:29:09.52 Carousel.configure :default @17:29:09.65 Pressure.configure :default @17:29:09.77 ExtraPump.configure :default @17:29:09.87 PinPort.configure :default @17:29:09.92 PinPort.hold @17:29:09.97 PinPort.configure :default @17:29:10.02 All elf controllers running firmware version 4.83 @17:29:10.60 Toroid.seek :clear @17:29:10.66 Carousel.in :default @17:29:10.76 Carousel.home.seek 4 @17:29:10.99 Selecting Cartridge 2 @17:29:11.22 Carousel.seek 2 @17:29:11.91 Lysis1.in :fast @17:29:11.96 Lysis1.seek :locked @17:29:14.53 Plunge.seek :locked @17:29:14.65 Air.in :fast @17:29:14.70 Air.seek :locked @17:29:16.26
Carousel.reconfigure :locked @17:29:16.38 Powering cartridge bus @17:29:18.99 Gate.setIR 1750,5000,0,6 @17:29:19.11 Gate.setIR 0,5000,0,6 @17:29:19.16 PuckHeater.configure :default @17:29:19.21 Gate.power :heat,:ON,limit to 5000mA @17:29:19.88 Mounted lysis Cartridge board#Q05086 @17:29:19.90 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:29:20.16 Preparing to filter cartridge 2 @17:29:20.40 Priming sample loop w/100ml, bypass w/10ml @17:29:20.41 SamplePump.setPosition! 0ml @17:29:20.44 Exhaust.close @17:29:20.50 Bypass.open @17:29:21.98 Intake.open @17:29:22.68 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:29:25.25 Vacuum tare is 60% of full scale @17:29:25.37 Pressure.in :default @17:29:25.42 Pressure.hold @17:29:25.59 SamplePump.in :prime @17:29:25.64 SamplePump.seek 10ml,59 seconds @17:29:34.36 Pressure.stop @17:29:34.40 SamplePump.setPosition! 0ml @17:29:34.44 Exhaust.open @17:29:35.88 Bypass.close @17:29:37.41 Pressure.in :default @17:29:37.46 Pressure.hold @17:29:37.68 SamplePump.in :prime @17:29:37.73 SamplePump.seek 100ml,5:16 @17:31:04.39 Pressure.stop @17:31:04.45 Bypass.open @17:31:05.16 Intake.close @17:31:05.87 Toroid.in :hold @17:31:05.92 Toroid.seek :crack @17:31:07.44 Toroid.seek :cartridge @17:31:08.53 Toroid.in :default @17:31:08.58 Toroid.seek :clear @17:31:10.53 Pressurizing cartridge to 26psi above 14.7psia @17:31:10.54 SamplePump.setPosition! 0ml @17:31:10.57 Intake.open @17:31:11.20 Bypass.close @17:31:11.89 Exhaust.close @17:31:12.68 Pressure.in :default @17:31:12.73 Pressure.hold @17:31:12.90 SamplePump.in :charge @17:31:12.96 SamplePump.seek 2.3ml,42 seconds @17:31:26.05 Pressure.stop @17:31:26.09 Intake.close @17:31:26.81 Pressurized cartridge with 0.818ml to 26.2psi @17:31:26.83 Isolated @38.9psia (24.2psi) @17:31:26.84 Verifying cartridge holds at least 0.65..0.85 of 38.9psia @17:31:26.85 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:31:31.49 Sampling 1000.0ml in 1:25:20 @17:31:31.50 SamplePump.setPosition! 0ml @17:31:31.93 Exhaust.open @17:31:32.67 Intake.open @17:31:33.49 Pressure.in :default @17:31:33.54 Pressure.hold @17:31:33.64 Sea 3.0m deep (19.1psia) @17:31:33.66 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @17:31:33.79 SamplePump.in :sample @17:31:33.84 SamplePump.seek 1000ml,1:23:51 @17:31:36.53 Cartridge 2 filtering 1/1000ml at 0.48ml/s with 23.4psi vacuum @17:32:36.55 Cartridge 2 filtering 31/1000ml at 0.50ml/s with 24.5psi vacuum @17:33:36.55 Cartridge 2 filtering 62/1000ml at 0.49ml/s with 23.1psi vacuum @17:34:36.55 Cartridge 2 filtering 92/1000ml at 0.52ml/s with 24.5psi vacuum @17:35:36.55 Cartridge 2 filtering 123/1000ml at 0.52ml/s with 24.4psi vacuum @17:36:36.54 Cartridge 2 filtering 154/1000ml at 0.52ml/s with 24.8psi vacuum @17:37:36.55 Cartridge 2 filtering 185/1000ml at 0.51ml/s with 24.0psi vacuum @17:38:36.55 Cartridge 2 filtering 216/1000ml at 0.53ml/s with 24.4psi vacuum @17:39:36.55 Cartridge 2 filtering 247/1000ml at 0.52ml/s with 25.2psi vacuum @17:40:36.55 Cartridge 2 filtering 278/1000ml at 0.52ml/s with 23.3psi vacuum @17:41:36.55 Cartridge 2 filtering 309/1000ml at 0.52ml/s with 24.8psi vacuum @17:42:36.54 Cartridge 2 filtering 341/1000ml at 0.52ml/s with 23.3psi vacuum @17:43:36.54 Cartridge 2 filtering 372/1000ml at 0.53ml/s with 25.3psi vacuum @17:44:36.55 Cartridge 2 filtering 404/1000ml at 0.52ml/s with 24.8psi vacuum @17:45:36.55 Cartridge 2 filtering 435/1000ml at 0.53ml/s with 23.6psi vacuum @17:46:36.55 Cartridge 2 filtering 467/1000ml at 0.53ml/s with 25.6psi vacuum @17:47:36.55 Cartridge 2 filtering 498/1000ml at 0.53ml/s with 23.4psi vacuum @17:48:36.55 Cartridge 2 filtering 530/1000ml at 0.53ml/s with 23.4psi vacuum @17:49:36.55 Cartridge 2 filtering 562/1000ml at 0.53ml/s with 23.9psi vacuum @17:50:36.55 Cartridge 2 filtering 594/1000ml at 0.53ml/s with 24.3psi vacuum @17:51:36.55 Cartridge 2 filtering 625/1000ml at 0.52ml/s with 23.4psi vacuum @17:52:36.55 Cartridge 2 filtering 658/1000ml at 0.53ml/s with 25.2psi vacuum @17:53:36.55 Cartridge 2 filtering 690/1000ml at 0.53ml/s with 22.7psi vacuum @17:54:36.55 Cartridge 2 filtering 721/1000ml at 0.53ml/s with 24.3psi vacuum @17:55:36.55 Cartridge 2 filtering 753/1000ml at 0.53ml/s with 24.9psi vacuum @17:56:36.55 Cartridge 2 filtering 785/1000ml at 0.53ml/s with 22.8psi vacuum 1.8m deep (17.4psia) @17:57:36.55 Cartridge 2 filtering 817/1000ml at 0.53ml/s with 23.8psi vacuum 2.3m deep (18.1psia) @17:58:36.55 Cartridge 2 filtering 849/1000ml at 0.54ml/s with 23.2psi vacuum 2.3m deep (18.1psia) @17:59:36.95 Cartridge 2 filtering 882/1000ml at 0.55ml/s with 24.4psi vacuum @18:00:36.54 Cartridge 2 filtering 914/1000ml at 0.54ml/s with 23.0psi vacuum @18:01:36.55 Cartridge 2 filtering 947/1000ml at 0.54ml/s with 24.9psi vacuum @18:02:36.55 Cartridge 2 filtering 979/1000ml at 0.54ml/s with 25.5psi vacuum @18:03:14.78 Pressure.stop @18:03:14.88 Intake.close @18:03:14.93 Exhaust.close @18:03:15.12 Toroid.in :hold @18:03:15.17 Toroid.seek :bypass @18:03:16.79 Toroid.in :default @18:03:16.84 Toroid.seek :clear @18:03:18.44 Sampled 1000.0ml @18:03:19.94 SPR.command "read_sequences" @18:03:20.40 SPR.command "stats_on" @18:03:20.53 SPR.command "start 0 1" @18:03:21.25 SPR.start /var/log/esp/spr23apr2618h.spr.bz2 @18:03:23.25 Handoff.select :waste @18:03:29.92 Syringe tare is 17% of full scale @18:03:29.94 PinPort.in :locking @18:03:29.99 PinPort.seek :locked @18:03:30.46 Archive.in :fast @18:03:30.52 Lysis2.in :fast @18:03:30.55 Lysis1.seek 1350uL @18:03:30.65 Archive.seek :full @18:03:30.70 Lysis2.seek :full @18:03:32.65 Plunge.seek :reagent @18:03:33.52 Evacuating puck with filter bubble point of 50psi @18:03:33.54 PinPort.seek :locked @18:03:33.60 Handoff.select :waste @18:03:40.18 Air syringe pumping up to 1 strokes to reach 8psi @18:03:40.19 Air stroke #1 of 1 @18:03:40.27 Air.in :pressurize @18:03:40.32 Air.seek :pressurized @18:03:46.03 0.7psi after pressurize @18:03:46.05 Air.in :compress @18:03:46.10 Air.seek :evac @18:03:49.08 1.1psi after compress @18:03:59.65 0.0psi after compress (stable) @18:03:59.68 Air.in :default @18:03:59.76 Air.hold @18:03:59.92 at 0.1psi Opened to waste @18:03:59.93 Handoff.select :blocked @18:04:00.04 Archive syringe pumping up to 8 strokes to reach 50psi @18:04:00.05 Archive stroke #1 of 8 @18:04:00.11 Archive.in :pressurize @18:04:00.16 Archive.seek :pressurized @18:04:12.62 0.4psi after pressurize @18:04:33.70 20.5psi after pressurize (stable) @18:04:33.74 Archive.in :compress @18:04:33.81 Archive.seek :empty @18:04:48.41 21.7psi after compress @18:04:58.99 22.3psi after compress (stable) @18:04:59.00 Archive.in :gulp @18:04:59.05 Archive.seek :topVent @18:05:05.28 23.2psi after gulp @18:05:11.06 23.5psi after gulp (stable) @18:05:11.07 Archive stroke #2 of 8 @18:05:11.13 Archive.in :pressurize @18:05:11.19 Archive.seek :pressurized @18:05:23.65 23.7psi after pressurize @18:05:29.23 24.1psi after pressurize (stable) @18:05:29.24 Archive.in :compress @18:05:29.29 Archive.seek :empty @18:05:43.89 27.3psi after compress @18:06:11.47 32.2psi after compress (stable) @18:06:11.48 Archive.in :gulp @18:06:11.53 Archive.seek :topVent @18:06:17.76 33.2psi after gulp @18:06:23.36 33.4psi after gulp (stable) @18:06:23.37 Archive stroke #3 of 8 @18:06:23.46 Archive.in :pressurize @18:06:23.54 Archive.seek :pressurized @18:06:36.00 33.1psi after pressurize @18:06:41.58 33.1psi after pressurize (stable) @18:06:41.60 Archive.in :compress @18:06:41.65 Archive.seek :empty @18:06:56.23 35.5psi after compress @18:07:06.81 36.1psi after compress (stable) @18:07:06.82 Archive.in :gulp @18:07:06.87 Archive.seek :topVent @18:07:13.10 37.2psi after gulp @18:07:18.67 37.2psi after gulp (stable) @18:07:18.68 Archive stroke #4 of 8 @18:07:18.75 Archive.in :pressurize @18:07:18.81 Archive.seek :pressurized @18:07:31.27 36.5psi after pressurize @18:07:36.84 36.5psi after pressurize (stable) @18:07:36.86 Archive.in :compress @18:07:36.91 Archive.seek :empty @18:07:51.82 39.9psi after compress @18:08:25.89 47.9psi after compress (stable) @18:08:25.91 Archive.in :gulp @18:08:25.96 Archive.seek :topVent @18:08:28.34 Reached 50.0 of 50.0psi after pumping 6920uL (4.00 strokes) @18:08:28.36 Archive.in :default @18:08:28.41 Archive.hold @18:08:28.46 Handoff.select :waste @18:08:40.67 delay 1:00 @18:09:40.68 Handoff.select :blocked @18:10:03.36 Handoff.select :waste @18:10:03.46 1.4psi at evacEnd @18:10:03.47 Handoff.select :blocked @18:10:03.58 Archive.in :fast @18:10:03.63 Archive.seek :topVent @18:10:15.18 Stable @0.3psi after Handoff blocked @18:10:15.21 Archive syringe pumping up to 8 strokes to reach 30psi @18:10:15.23 Archive stroke #1 of 8 @18:10:15.29 Archive.in :pressurize @18:10:15.35 Archive.seek :pressurized @18:10:27.83 0.5psi after pressurize @18:10:27.84 Archive.in :compress @18:10:27.89 Archive.seek :empty @18:10:42.49 2.5psi after compress @18:11:03.06 6.9psi after compress (stable) @18:11:03.08 Archive.in :gulp @18:11:03.13 Archive.seek :topVent @18:11:09.35 7.7psi after gulp @18:11:14.92 8.1psi after gulp (stable) @18:11:14.94 Archive stroke #2 of 8 @18:11:15.00 Archive.in :pressurize @18:11:15.05 Archive.seek :pressurized @18:11:27.53 8.7psi after pressurize @18:11:27.55 Archive.in :compress @18:11:27.59 Archive.seek :empty @18:11:42.20 16.2psi after compress @18:12:04.29 22.9psi after compress (stable) @18:12:04.30 Archive.in :gulp @18:12:04.35 Archive.seek :topVent @18:12:10.60 23.7psi after gulp @18:12:16.18 24.0psi after gulp (stable) @18:12:16.19 Archive stroke #3 of 8 @18:12:16.27 Archive.in :pressurize @18:12:16.34 Archive.seek :pressurized @18:12:28.82 24.7psi after pressurize @18:12:28.84 Archive.in :compress @18:12:28.88 Archive.seek :empty @18:12:42.84 Reached 30.4 of 30.3psi after pumping 5175uL (2.99 strokes) @18:12:42.86 Archive.in :default @18:12:42.91 Archive.hold @18:12:42.96 Handoff.select :waste @18:12:50.14 delay 1:00 @18:13:50.15 Handoff.select :blocked @18:14:12.82 Handoff.select :waste @18:14:12.93 0.9psi at evacEnd @18:14:12.95 Air.seek :fullVent @18:14:14.35 Archive.in :fast @18:14:14.40 Archive.seek :topVent @18:14:21.44 Seawater evacuated, Pressure above/below filter released. @18:14:21.51 Lysis1.in :slow @18:14:21.57 Lysis1.seek 1050uL @18:14:51.57 delay 5 seconds @18:14:56.59 Handoff.select :blocked @18:14:56.76 Lysis1.seek 450uL @18:15:56.70 delay 3 seconds @18:15:59.78 Air.in mix with 1.40048 parts Lysis1 @18:16:00.27 Air.seek :evac @18:16:00.31 Lysis1.seek 300uL @18:16:15.41 Lysis1.seek :empty @18:16:45.49 Lysis buffer delivered @15.1psi @18:16:45.50 Polling Can every 2:00 @18:16:46.04 PuckHeater.configure :default #ambient 22.6C @18:16:46.07 PuckHeater.seek 79.7C @18:17:41.26 PuckHeater.hold 2:00,79.7C @18:19:40.98 Polling Can every 2:00 @18:19:41.52 PuckHeater.configure :default #ambient 22.6C @18:19:41.56 PuckHeater.hold 30 seconds,78.7C @18:20:11.66 Polling Can every 2:00 @18:20:12.20 PuckHeater.configure :default #ambient 22.6C @18:20:12.24 PuckHeater.hold 30 seconds,78.1C @18:20:42.35 Polling Can every 2:00 @18:20:42.88 PuckHeater.configure :default #ambient 22.6C @18:20:42.93 PuckHeater.hold 10:00,77.6C @18:30:41.32 Lysis complete. Syringe pressure @19.9psi @18:30:41.34 Archive syringe pumping up to 5 strokes to reach 40psi @18:30:41.35 Archive stroke #1 of 5 @18:30:41.41 Archive.in :pressurize @18:30:41.46 Archive.seek :pressurized @18:30:53.93 19.5psi after pressurize @18:30:59.50 19.4psi after pressurize (stable) @18:30:59.52 Archive.in :compress @18:30:59.57 Archive.seek :empty @18:31:06.50 Reached 40.4 of 40.0psi after pumping 1564uL (0.90 strokes) @18:31:06.53 Archive.in :default @18:31:06.59 Archive.hold @18:31:06.65 delay 2 seconds @18:31:08.67 Archive.in :fast @18:31:08.72 Archive.seek :full @18:31:15.30 Lysis1.seek 138uL @18:31:29.15 delay 1 second @18:31:30.18 Archive.seek :full @18:31:30.23 Lysis1.seek :empty @18:31:44.13 Handoff.select :blocked @18:31:44.22 Initial air pressure @42.4psi @18:31:44.29 Lysis1.in :extract @18:31:44.38 Lysis1.seek 600uL,nil @18:31:47.31 Archive syringe pumping up to 6 strokes to reach 25psi @18:31:47.32 Archive stroke #1 of 6 @18:31:47.40 Archive.in :pressurize @18:31:47.45 Archive.seek :pressurized @18:31:59.98 11.9psi after pressurize @18:32:10.83 Archive.in :default @18:32:10.89 Archive.hold @18:32:11.03 Initial 600uL lysate @16.2psi @18:32:11.04 Handoff.select :blocked @18:32:11.12 Initial air pressure @16.3psi @18:32:11.19 Lysis1.seek 825uL,nil @18:32:12.95 Archive syringe pumping up to 6 strokes to reach 17psi @18:32:12.96 Archive stroke #1 of 6 @18:32:13.03 Archive.in :compress @18:32:13.09 Archive.seek :empty @18:32:20.20 Archive.in :default @18:32:20.59 Archive.hold @18:32:20.91 Recovered 825uL lysate @16.5psi @18:32:20.93 Air.in :halfSpeed @18:32:20.97 Air.seek :fullVent @18:32:23.19 Puck Top Vented, bottom @5.8psi @18:32:23.20 Handoff.select :waste @18:32:23.30 delay 5 seconds @18:32:28.37 Handoff open to waste, bottom @0.1psi @18:32:28.38 Air.in :default @18:32:28.43 Air.seek :evac @18:32:29.84 Lysis2.in :DAmix @18:32:29.94 Lysis1.in :DAmix @18:32:30.35 Lysis2.seek 1480uL @18:32:30.38 Lysis1.seek 575uL @18:33:05.44 delay 5 seconds @18:33:10.45 Handoff.select SPR @18:33:10.57 SPR.command "start 2 14" @18:33:11.82 SPR stabilized @18:33:11.94 delay 10 seconds @18:33:21.96 SPR.command "start 1 0" @18:33:25.18 Waiting up to 4:00 for SPR to enter state 1 @18:35:29.48 SPR.command "hold_on" @18:35:31.38 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:35:31.86 Lysis2.seek 1180uL @18:35:31.91 Lysis1.seek 275uL @18:36:13.88 delay 15 seconds @18:36:28.91 SPR.command "hold_off" @18:36:31.51 Waiting up to 38:00 for SPR to enter state 7 @18:54:30.18 SPRsummary:-2.46RIU,192RIU,178RIU,285RIU,none,none,none after 5542s @19:01:07.40 SPR analysis completed /home/esp/esp2/lib/schedule.rb:558:in `terminate!': Thread(0xb6a05e80): deadlock (fatal) from /home/esp/esp2/lib/log.rb:169:in `initialize' /home/esp/esp2/lib/schedule.rb:558:in `synchronize': Thread(0xb6a05e80): deadlock (fatal) from /home/esp/esp2/lib/log.rb:435:in `append' from /home/esp/esp2/lib/log.rb:482:in `recordObject' from /home/esp/esp2/lib/log.rb:501:in `recordException' from /home/esp/esp2/lib/log.rb:636:in `recordException' from /home/esp/esp2/lib/rmutex.rb:99:in `synchronize' from /home/esp/esp2/lib/log.rb:635:in `recordException' from /home/esp/esp2/lib/log.rb:259:in `recordException' from /home/esp/esp2/lib/esp.rb:91:in `mainThread' from /home/esp/esp2/lib/esp.rb:360 @12:21:13.24PDT27-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @12:21:13.26 >BREAK< ESP3 Gateway v1.42 for cartridge bus @12:21:15.83 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @12:21:16.30 Sea pressure (Abs) tare is 6% of full scale @12:21:22.35 Polling Can every 2:00 @12:21:22.39 Loading /home/esp/esp2/bin/processAll mission @12:21:22.56 Sea pressure (Abs) tare is 6% of full scale Sampling default goal volumes @12:21:22.57 Cartridge group: dry Spares: {spare: 0} @12:21:22.72 Gate.power :analytic1,:ON,limit to 5000mA @12:21:22.73 Gate.power :analytic2,:ON,limit to 5000mA @12:21:22.74 Gate.power :analytic3,:ON,limit to 5000mA @12:21:23.32 Archive.configure :default @12:21:23.39 Archive.hold @12:21:23.46 Archive.configure :default @12:21:23.58 Air.configure :default @12:21:23.63 Air.hold @12:21:23.68 Air.configure :default @12:21:23.79 Lysis1.configure :default @12:21:23.84 Lysis1.hold @12:21:23.89 Lysis1.configure :default @12:21:23.99 Lysis2.configure :default @12:21:24.04 Lysis2.hold @12:21:24.09 Lysis2.configure :default @12:21:24.21 Plunge.configure :default @12:21:24.26 Plunge.hold @12:21:24.31 Plunge.configure :default @12:21:24.44 SamplePump.configure :default @12:21:24.57 Toroid.configure :default @12:21:24.62 Toroid.hold @12:21:24.94 Toroid.configure :default @12:21:25.06 Carousel.configure :default @12:21:25.47 Carousel.hold @12:21:25.53 Carousel.configure :default @12:21:25.66 Pressure.configure :default @12:21:25.78 ExtraPump.configure :default @12:21:25.88 PinPort.configure :default @12:21:25.93 PinPort.hold @12:21:25.98 PinPort.configure :default @12:21:26.03 All elf controllers running firmware version 4.83 @12:21:26.32 SPR Module 1, FW Rev. B, FPGA Rev. C @12:21:26.92
Toroid.seek :clear @12:21:26.98 Carousel.in :default @12:21:27.08 Carousel.home.seek 2 @12:21:27.43 Carousel.reconfigure :locked @12:21:27.60 Gate.setIR 1750,5000,0,6 @12:21:27.72 Gate.setIR 0,5000,0,6 @12:21:27.77 PuckHeater.configure :default @12:21:27.82 Gate.power :heat,:ON,limit to 5000mA @12:21:28.50 Mounted lysis Cartridge board#Q05086 @12:21:28.53 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:21:28.56 Selecting Cartridge 3 @12:21:28.68 Gate.power :heat,:OFF @12:21:28.69 Gate.power :cartridge,:OFF @12:21:28.76 PinPort.in :unlocking @12:21:28.81 PinPort.seek :clear @12:21:29.26 Carousel.in :default @12:21:29.37 Carousel.home.seek 2 @12:21:29.66 Archive.in :unlocking @12:21:29.78 Archive.seek :clear @12:21:29.83 Air.in :unlocking @12:21:29.96 Air.seek :clear @12:21:30.05 Lysis2.in :unlocking @12:21:30.18 Lysis2.seek :clear @12:21:34.12 Lysis1.in :unlocking @12:21:34.25 Lysis1.seek :clear @12:21:34.31 Plunge.in :unlocking @12:21:34.39 Plunge.seek :clear @12:21:38.14
Carousel.seek 3 @12:21:38.60 Lysis1.in :fast @12:21:38.65 Lysis1.seek :locked @12:21:41.24 Plunge.in :default @12:21:41.68 Plunge.seek :locked @12:21:41.78 Air.in :fast @12:21:41.83 Air.seek :locked @12:21:43.39
Carousel.reconfigure :locked @12:21:43.52 Powering cartridge bus @12:21:46.13 Gate.setIR 1750,5000,0,6 @12:21:46.25 Gate.setIR 0,5000,0,6 @12:21:46.29 Gate.power :cartridge,:OFF @12:21:46.82 Mounted archive Cartridge @12:21:47.07 Preparing to filter cartridge 3 @12:21:47.30 Priming sample loop w/100ml, bypass w/10ml @12:21:47.31 SamplePump.setPosition! 0ml @12:21:47.34 Exhaust.close @12:21:47.42 Bypass.open @12:21:48.89 Intake.open @12:21:49.62 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @12:21:52.18 Vacuum tare is 60% of full scale @12:21:52.30 Pressure.in :default @12:21:52.35 Pressure.hold @12:21:52.52 SamplePump.in :prime @12:21:52.58 SamplePump.seek 10ml,59 seconds @12:22:01.29 Pressure.stop @12:22:01.33 SamplePump.setPosition! 0ml @12:22:01.37 Exhaust.open @12:22:02.81 Bypass.close @12:22:04.33 Pressure.in :default @12:22:04.38 Pressure.hold @12:22:04.61 SamplePump.in :prime @12:22:04.66 SamplePump.seek 100ml,5:16 @12:23:34.52 Pressure.stop @12:23:34.58 Bypass.open @12:23:35.28 Intake.close @12:23:36.00 Toroid.in :hold @12:23:36.05 Toroid.seek :crack @12:23:37.57 Toroid.seek :cartridge @12:23:38.66 Toroid.in :default @12:23:38.71 Toroid.seek :clear @12:23:41.04 Pressurizing cartridge to 26psi above 15.3psia @12:23:41.05 SamplePump.setPosition! 0ml @12:23:41.08 Intake.open @12:23:41.72 Bypass.close @12:23:42.39 Exhaust.close @12:23:43.18 Pressure.in :default @12:23:43.23 Pressure.hold @12:23:43.40 SamplePump.in :charge @12:23:43.46 SamplePump.seek 2.3ml,42 seconds @12:23:52.51 Pressure.stop @12:23:52.55 Intake.close @12:23:53.29 Pressurized cartridge with 0.389ml to 26.5psi @12:23:53.30 Isolated @41.0psia (25.7psi) @12:23:53.31 Verifying cartridge holds at least 0.65..0.85 of 41.0psia @12:23:53.33 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @12:23:57.97 Sampling 1000.0ml in 1:25:20 @12:23:57.99 SamplePump.setPosition! 0ml @12:23:58.03 Exhaust.open @12:23:58.76 Intake.open @12:23:59.53 Pressure.in :default @12:23:59.58 Pressure.hold @12:23:59.69 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @12:23:59.83 SamplePump.in :sample @12:23:59.88 SamplePump.seek 1000ml,1:23:51 @12:24:02.58 Cartridge 3 filtering 1/1000ml at 0.49ml/s with 24.0psi vacuum @12:25:02.59 Cartridge 3 filtering 30/1000ml at 0.48ml/s with 25.5psi vacuum @12:26:02.59 Cartridge 3 filtering 58/1000ml at 0.46ml/s with 25.0psi vacuum @12:27:02.59 Cartridge 3 filtering 84/1000ml at 0.43ml/s with 26.5psi vacuum @12:28:02.59 Cartridge 3 filtering 111/1000ml at 0.44ml/s with 23.6psi vacuum @12:29:02.58 Cartridge 3 filtering 136/1000ml at 0.41ml/s with 23.9psi vacuum @12:30:02.59 Cartridge 3 filtering 161/1000ml at 0.42ml/s with 22.7psi vacuum @12:31:02.59 Cartridge 3 filtering 185/1000ml at 0.41ml/s with 24.8psi vacuum @12:32:02.59 Cartridge 3 filtering 208/1000ml at 0.39ml/s with 26.1psi vacuum @12:33:02.59 Cartridge 3 filtering 231/1000ml at 0.36ml/s with 23.0psi vacuum @12:34:02.58 Cartridge 3 filtering 253/1000ml at 0.35ml/s with 23.3psi vacuum @12:35:02.59 Cartridge 3 filtering 274/1000ml at 0.34ml/s with 23.8psi vacuum @12:36:02.99 Cartridge 3 filtering 295/1000ml at 0.32ml/s with 23.4psi vacuum @12:37:02.59 Cartridge 3 filtering 315/1000ml at 0.31ml/s with 22.4psi vacuum @12:38:02.59 Cartridge 3 filtering 334/1000ml at 0.31ml/s with 24.4psi vacuum @12:39:02.59 Cartridge 3 filtering 352/1000ml at 0.32ml/s with 24.1psi vacuum @12:40:02.59 Cartridge 3 filtering 370/1000ml at 0.29ml/s with 25.9psi vacuum @12:41:02.59 Cartridge 3 filtering 387/1000ml at 0.28ml/s with 24.6psi vacuum @12:42:02.59 Cartridge 3 filtering 404/1000ml at 0.27ml/s with 25.0psi vacuum @12:43:02.59 Cartridge 3 filtering 421/1000ml at 0.27ml/s with 24.8psi vacuum @12:44:02.59 Cartridge 3 filtering 437/1000ml at 0.26ml/s with 25.6psi vacuum @12:45:02.59 Cartridge 3 filtering 452/1000ml at 0.24ml/s with 24.5psi vacuum @12:46:02.59 Cartridge 3 filtering 467/1000ml at 0.25ml/s with 25.5psi vacuum @12:47:02.59 Cartridge 3 filtering 481/1000ml at 0.23ml/s with 26.2psi vacuum @12:48:02.59 Cartridge 3 filtering 495/1000ml at 0.22ml/s with 25.2psi vacuum @12:49:02.59 Cartridge 3 filtering 509/1000ml at 0.22ml/s with 24.5psi vacuum @12:50:02.59 Cartridge 3 filtering 522/1000ml at 0.21ml/s with 26.6psi vacuum @12:51:02.59 Cartridge 3 filtering 535/1000ml at 0.21ml/s with 25.1psi vacuum @12:52:02.59 Cartridge 3 filtering 547/1000ml at 0.22ml/s with 23.7psi vacuum @12:53:02.59 Cartridge 3 filtering 560/1000ml at 0.22ml/s with 24.1psi vacuum @12:54:02.59 Cartridge 3 filtering 572/1000ml at 0.20ml/s with 25.9psi vacuum @12:55:02.59 Cartridge 3 filtering 584/1000ml at 0.20ml/s with 26.4psi vacuum @12:55:07.98 Flow averaged 0.31ml/s over 31:09 @12:55:08.01 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @12:55:08.59 Relieved excess vacuum in .6 seconds @12:55:08.61 SamplePump.seek 1000ml,35:05 @12:55:11.54 Flow estimate reduced to 0.25ml/s @12:55:11.58 Waiting up to 12 seconds for vacuum to reduce from 27.6 to 18.0psi @12:55:12.15 Relieved excess vacuum in .6 seconds @12:55:12.18 SamplePump.seek 1000ml,35:02 @12:55:15.97 Flow estimate reduced to 0.20ml/s @12:55:16.00 Waiting up to 8 seconds for vacuum to reduce from 26.2 to 18.0psi @12:55:16.58 Relieved excess vacuum in .6 seconds @12:55:16.60 SamplePump.seek 1000ml,34:58 @12:55:25.14 Flow estimate reduced to 0.16ml/s @12:55:25.16 Intake.close @12:55:25.20 Exhaust.close @12:55:25.44 Toroid.in :hold @12:55:25.50 Toroid.seek :bypass @12:55:27.12 Toroid.in :default @12:55:27.18 Toroid.seek :clear @12:55:28.79 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @12:55:28.80 Pressure.stop @12:55:29.39 Sampled 588.1ml @12:55:29.88 Plunge.seek :reagent @12:55:30.68 Sample loop closed. Ready to Deliver Reagents @12:55:30.69 Archive.in :default @12:55:30.74 Archive.seek :sealed @12:55:34.66 Archive.seek :evac @12:55:48.68 delay 1:00 @12:56:48.71 Archive.seek :clear @12:57:06.18 RNAlater soak for 10:00 @13:07:06.26 Archive.seek :tVent @13:07:08.69 delay 1 second @13:07:09.71 Archive.seek :evac @13:07:25.21 delay 10 seconds @13:07:35.24 Sample preserved and evacuated @13:07:35.44
Carousel.in :default @13:07:35.54 Carousel.home.seek 3 @13:07:35.83 Plunge.seek :locked @13:07:36.69 Archive.in :unlocking @13:07:36.81 Archive.seek :clear @13:07:36.85 Air.in :unlocking @13:07:36.99 Air.seek :clear @13:07:37.06 Lysis1.in :unlocking @13:07:37.19 Lysis1.seek :clear @13:07:41.44
Gate.power :motor,:OFF @13:07:41.45 Gate.power :core,:OFF @13:07:41.46 Gate.power :analytic1,:OFF @13:07:41.47 Gate.power :analytic2,:OFF @13:07:41.48 Gate.power :analytic3,:OFF @13:07:41.49 Gate.power :main,:OFF @13:07:41.50 Safely stopped and ready to power off @13:07:45.27 Gate.power :main,:ON,limit to 5000mA @13:07:45.28 Gate.power :analytic1,:ON,limit to 5000mA @13:07:45.29 Gate.power :analytic2,:ON,limit to 5000mA @13:07:45.31 Gate.power :analytic3,:ON,limit to 5000mA @13:07:45.36 Powering core bus @13:07:47.71 SPR Module 1, FW Rev. B, FPGA Rev. C @13:07:48.05
Archive.configure :default @13:07:48.13 Air.configure :default @13:07:48.18 Lysis1.configure :default @13:07:48.23 Lysis2.configure :default @13:07:48.31 Plunge.configure :default @13:07:48.38 SamplePump.configure :default @13:07:48.46 Toroid.configure :default @13:07:48.53 Carousel.configure :default @13:07:48.61 Pressure.configure :default @13:07:48.68 ExtraPump.configure :default @13:07:48.73 PinPort.configure :default @13:07:48.78 All elf controllers running firmware version 4.83 @13:07:48.80 Gate.power :motor,:ON,limit to 5000mA @13:07:48.95 Archive.home.jog 0 @13:07:49.93 Carousel.home.jog 0 @13:07:49.98 Toroid.home.jog 0 @13:07:50.00 Lysis1.home.jog 0 @13:07:50.03 Lysis2.home.jog 0 @13:07:50.08 PinPort.home.jog 0 @13:07:50.11 Air.home.jog 0 @13:07:50.13 Plunge.home.jog 0 @13:07:50.72 Archive.in :homing @13:07:50.86 Archive.jog 1382 @13:07:51.04 Toroid.in :homing @13:07:51.07 Lysis2.in :homing @13:07:51.10 Lysis1.in :homing @13:07:51.14 Air.in :homing @13:07:51.17 PinPort.in :homing @13:07:51.20 Plunge.in :homing @13:07:51.36 Lysis2.jog 1392 @13:07:51.44 Toroid.jog 619 @13:07:51.47 Lysis1.jog 1400 @13:07:51.49 Air.jog 1376 @13:07:51.51 PinPort.jog -106 @13:07:51.57 Plunge.jog 1547 @13:07:51.89 PinPort.setRawPosition! 0 @13:07:51.93 PinPort.seek :home @13:07:52.00 PinPort.in :default @13:07:52.07 Archive.setRawPosition! 50 @13:07:52.12 Archive.seek :home @13:07:52.38 Archive.in :default @13:07:52.70 Lysis2.setRawPosition! 50 @13:07:52.74 Lysis2.seek :home @13:07:52.83 Lysis1.setRawPosition! 50 @13:07:52.85 Air.setRawPosition! 50 @13:07:52.91 Lysis1.seek :home @13:07:53.00 Air.seek :home @13:07:53.07 Plunge.setRawPosition! 50 @13:07:53.10 Lysis2.in :default @13:07:53.17 Plunge.seek :home @13:07:53.28 Lysis1.in :default @13:07:53.36 Air.in :default @13:07:53.46 Plunge.in :default @13:07:53.64 Toroid.setRawPosition! 0 @13:07:53.68 Toroid.seek :home @13:07:53.75 Toroid.in :default @13:07:53.82
Toroid.seek :clear @13:07:55.43 Carousel.in :default @13:07:55.53 Carousel.home.seek 3 @13:07:55.76 Selecting Cartridge 1 @13:07:56.00 Carousel.seek 1 @13:07:56.68 Lysis1.in :fast @13:07:56.72 Lysis1.seek :locked @13:07:59.30 Plunge.seek :locked @13:07:59.43 Air.in :fast @13:07:59.48 Air.seek :locked @13:08:01.34
Carousel.reconfigure :locked @13:08:01.46 Powering cartridge bus @13:08:04.08 Gate.setIR 1750,5000,0,6 @13:08:04.19 Gate.setIR 0,5000,0,6 @13:08:04.25 PuckHeater.configure :default @13:08:04.29 Gate.power :heat,:ON,limit to 5000mA @13:08:04.94 Mounted lysis Cartridge board#Q05034 @13:08:04.97 Using this cartridge's heat loss curve for HeatingAccelInCarousel @13:08:05.22 Preparing to filter cartridge 1 @13:08:05.45 Priming sample loop w/100ml, bypass w/10ml @13:08:05.46 SamplePump.setPosition! 0ml @13:08:05.49 Bypass.open @13:08:06.93 Intake.open @13:08:07.65 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:08:10.22 Vacuum tare is 60% of full scale @13:08:10.34 Pressure.in :default @13:08:10.39 Pressure.hold @13:08:10.55 SamplePump.in :prime @13:08:10.61 SamplePump.seek 10ml,59 seconds @13:08:19.34 Pressure.stop @13:08:19.37 SamplePump.setPosition! 0ml @13:08:19.41 Exhaust.open @13:08:20.86 Bypass.close @13:08:22.37 Pressure.in :default @13:08:22.42 Pressure.hold @13:08:22.64 SamplePump.in :prime @13:08:22.70 SamplePump.seek 100ml,5:16 @13:09:51.62 Pressure.stop @13:09:51.68 Bypass.open @13:09:52.40 Intake.close @13:09:53.13 Toroid.in :hold @13:09:53.18 Toroid.seek :crack @13:09:54.70 Toroid.seek :cartridge @13:09:55.79 Toroid.in :default @13:09:55.84 Toroid.seek :clear @13:09:57.79 Pressurizing cartridge to 26psi above 15.6psia @13:09:57.80 SamplePump.setPosition! 0ml @13:09:58.15 Intake.open @13:09:58.86 Bypass.close @13:09:59.53 Exhaust.close @13:10:00.33 Pressure.in :default @13:10:00.38 Pressure.hold @13:10:00.55 SamplePump.in :charge @13:10:00.60 SamplePump.seek 2.3ml,42 seconds @13:10:15.07 Pressure.stop @13:10:15.12 Intake.close @13:10:15.84 Pressurized cartridge with 1.001ml to 26.2psi @13:10:15.85 Isolated @40.4psia (24.8psi) @13:10:15.86 Verifying cartridge holds at least 0.65..0.85 of 40.4psia @13:10:15.88 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @13:10:20.45 Cartridge pressure rose 1% to 40.7psia @13:10:20.53 Sampling 1000.0ml in 1:25:20 @13:10:20.54 SamplePump.setPosition! 0ml @13:10:20.58 Exhaust.open @13:10:21.31 Intake.open @13:10:22.13 Pressure.in :default @13:10:22.18 Pressure.hold @13:10:22.29 Sea 4.4m deep (21.2psia) @13:10:22.30 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @13:10:22.43 SamplePump.in :sample @13:10:22.48 SamplePump.seek 1000ml,1:23:51 @13:10:25.18 Cartridge 1 filtering 1/1000ml at 0.46ml/s with 26.2psi vacuum @13:11:25.18 Cartridge 1 filtering 29/1000ml at 0.47ml/s with 24.1psi vacuum @13:12:25.19 Cartridge 1 filtering 57/1000ml at 0.45ml/s with 24.9psi vacuum @13:13:25.19 Cartridge 1 filtering 83/1000ml at 0.43ml/s with 24.3psi vacuum @13:14:25.19 Cartridge 1 filtering 109/1000ml at 0.43ml/s with 24.8psi vacuum @13:15:25.19 Cartridge 1 filtering 134/1000ml at 0.41ml/s with 23.5psi vacuum @13:16:25.18 Cartridge 1 filtering 158/1000ml at 0.37ml/s with 24.6psi vacuum @13:17:25.19 Cartridge 1 filtering 181/1000ml at 0.39ml/s with 25.0psi vacuum @13:18:25.18 Cartridge 1 filtering 204/1000ml at 0.37ml/s with 23.4psi vacuum @13:19:25.19 Cartridge 1 filtering 226/1000ml at 0.35ml/s with 24.3psi vacuum @13:20:25.18 Cartridge 1 filtering 247/1000ml at 0.35ml/s with 24.1psi vacuum @13:21:25.19 Cartridge 1 filtering 267/1000ml at 0.32ml/s with 24.4psi vacuum @13:22:25.19 Cartridge 1 filtering 286/1000ml at 0.31ml/s with 25.5psi vacuum @13:23:25.18 Cartridge 1 filtering 304/1000ml at 0.31ml/s with 24.4psi vacuum @13:24:25.19 Cartridge 1 filtering 322/1000ml at 0.29ml/s with 25.6psi vacuum @13:25:25.19 Cartridge 1 filtering 340/1000ml at 0.26ml/s with 24.7psi vacuum @13:26:25.19 Cartridge 1 filtering 357/1000ml at 0.28ml/s with 23.4psi vacuum @13:27:25.19 Cartridge 1 filtering 373/1000ml at 0.28ml/s with 24.7psi vacuum @13:28:25.18 Cartridge 1 filtering 390/1000ml at 0.26ml/s with 24.0psi vacuum @13:29:25.19 Cartridge 1 filtering 405/1000ml at 0.25ml/s with 25.2psi vacuum @13:30:25.19 Cartridge 1 filtering 421/1000ml at 0.25ml/s with 23.6psi vacuum @13:31:25.19 Cartridge 1 filtering 435/1000ml at 0.22ml/s with 23.9psi vacuum @13:32:25.19 Cartridge 1 filtering 449/1000ml at 0.24ml/s with 24.2psi vacuum @13:33:25.19 Cartridge 1 filtering 462/1000ml at 0.22ml/s with 23.3psi vacuum @13:34:25.19 Cartridge 1 filtering 475/1000ml at 0.20ml/s with 24.7psi vacuum @13:35:25.19 Cartridge 1 filtering 488/1000ml at 0.22ml/s with 22.9psi vacuum 1.6m deep (17.1psia) @13:36:25.19 Cartridge 1 filtering 501/1000ml at 0.20ml/s with 23.5psi vacuum @13:36:28.97 Session Begun @13:36:35.79 -> showlog -200 @13:36:55.09 -> showlog @13:37:25.19 Cartridge 1 filtering 513/1000ml at 0.20ml/s with 25.4psi vacuum @13:38:25.18 Cartridge 1 filtering 525/1000ml at 0.20ml/s with 25.8psi vacuum @13:39:25.18 Cartridge 1 filtering 537/1000ml at 0.20ml/s with 25.2psi vacuum @13:40:18.32 Flow averaged 0.30ml/s over 29:56 @13:40:18.36 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @13:40:19.20 Relieved excess vacuum in .9 seconds @13:40:19.23 SamplePump.seek 1000ml,38:13 @13:40:21.25 Flow estimate reduced to 0.24ml/s @13:40:21.30 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @13:40:22.13 Relieved excess vacuum in .9 seconds @13:40:22.16 SamplePump.seek 1000ml,38:11 @13:40:24.15 Flow estimate reduced to 0.20ml/s @13:40:24.17 Intake.close @13:40:24.24 Exhaust.close @13:40:24.46 Toroid.in :hold @13:40:24.53 Toroid.seek :bypass @13:40:25.18 Cartridge 1 filtering 548/1000ml at 0.00ml/s with 8.1psi vacuum 2.7m deep (18.7psia) @13:40:26.17 Toroid.in :default @13:40:26.25 Toroid.seek :clear @13:40:27.87 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @13:40:27.88 Pressure.stop @13:40:28.50 Sampled 548.4ml @13:40:30.05 SPR.command "read_sequences" @13:40:30.20 SPR.command "stats_on" @13:40:30.35 SPR.command "start 0 1" @13:40:31.07 SPR.start /var/log/esp/spr23apr2713h.spr.bz2 @13:40:33.06 Handoff.select :waste @13:40:39.76 Syringe tare is 18% of full scale @13:40:39.78 PinPort.in :locking @13:40:39.87 PinPort.seek :locked @13:40:40.35 Archive.in :fast @13:40:40.44 Lysis2.in :fast @13:40:40.48 Lysis1.seek 1350uL @13:40:40.55 Archive.seek :full @13:40:40.66 Lysis2.seek :full @13:40:42.65 Plunge.seek :reagent @13:40:43.54 Evacuating puck with filter bubble point of 50psi @13:40:43.56 PinPort.seek :locked @13:40:43.67 Handoff.select :waste @13:40:50.25 Air syringe pumping up to 1 strokes to reach 8psi @13:40:50.26 Air stroke #1 of 1 @13:40:50.37 Air.in :pressurize @13:40:50.79 Air.seek :pressurized @13:40:56.61 0.8psi after pressurize @13:40:56.63 Air.in :compress @13:40:56.71 Air.seek :evac @13:40:59.72 0.7psi after compress @13:41:10.30 -0.0psi after compress (stable) @13:41:10.31 Air.in :default @13:41:10.39 Air.hold @13:41:10.62 at -0.1psi Opened to waste @13:41:10.64 Handoff.select :blocked @13:41:10.78 Archive syringe pumping up to 8 strokes to reach 50psi @13:41:10.79 Archive stroke #1 of 8 @13:41:10.88 Archive.in :pressurize @13:41:10.96 Archive.seek :pressurized @13:41:23.44 0.0psi after pressurize @13:41:29.01 0.1psi after pressurize (stable) @13:41:29.03 Archive.in :compress @13:41:29.13 Archive.seek :empty @13:41:43.74 25.8psi after compress @13:41:54.32 27.1psi after compress (stable) @13:41:54.33 Archive.in :gulp @13:41:54.42 Archive.seek :topVent @13:42:00.67 28.1psi after gulp @13:42:06.27 28.3psi after gulp (stable) @13:42:06.29 Archive stroke #2 of 8 @13:42:06.40 Archive.in :pressurize @13:42:06.47 Archive.seek :pressurized @13:42:18.97 27.8psi after pressurize @13:42:24.55 27.9psi after pressurize (stable) @13:42:24.57 Archive.in :compress @13:42:24.65 Archive.seek :empty @13:42:39.26 31.3psi after compress @13:43:02.34 35.9psi after compress (stable) @13:43:02.36 Archive.in :gulp @13:43:02.45 Archive.seek :topVent @13:43:08.73 37.9psi after gulp @13:43:14.31 38.0psi after gulp (stable) @13:43:14.34 Archive stroke #3 of 8 @13:43:14.46 Archive.in :pressurize @13:43:14.91 Archive.seek :pressurized @13:43:27.40 37.0psi after pressurize @13:43:32.98 37.0psi after pressurize (stable) @13:43:33.00 Archive.in :compress @13:43:33.09 Archive.seek :empty @13:43:47.70 39.2psi after compress @13:44:26.88 Reached 50.0 of 50.0psi after pumping 5190uL (3.00 strokes) @13:44:26.89 Archive.in :default @13:44:26.97 Archive.hold @13:44:27.05 Handoff.select :waste @13:44:41.75 delay 1:00 @13:45:41.77 Handoff.select :blocked @13:46:04.47 Handoff.select :waste @13:46:04.60 1.5psi at evacEnd @13:46:04.61 Handoff.select :blocked @13:46:04.75 Archive.in :fast @13:46:04.81 Archive.seek :topVent @13:46:18.49 Stable @0.6psi after Handoff blocked @13:46:18.52 Archive syringe pumping up to 8 strokes to reach 31psi @13:46:18.54 Archive stroke #1 of 8 @13:46:18.65 Archive.in :pressurize @13:46:18.72 Archive.seek :pressurized @13:46:31.54 1.0psi after pressurize @13:46:31.56 Archive.in :compress @13:46:31.65 Archive.seek :empty @13:46:46.29 7.3psi after compress @13:47:06.38 13.7psi after compress (stable) @13:47:06.40 Archive.in :gulp @13:47:06.49 Archive.seek :topVent @13:47:12.77 14.5psi after gulp @13:47:18.36 14.8psi after gulp (stable) @13:47:18.38 Archive stroke #2 of 8 @13:47:18.49 Archive.in :pressurize @13:47:18.57 Archive.seek :pressurized @13:47:31.04 15.5psi after pressurize @13:47:31.07 Archive.in :compress @13:47:31.15 Archive.seek :empty @13:47:45.77 20.1psi after compress @13:48:06.37 26.5psi after compress (stable) @13:48:06.39 Archive.in :gulp @13:48:06.48 Archive.seek :topVent @13:48:12.73 27.2psi after gulp @13:48:18.31 27.4psi after gulp (stable) @13:48:18.32 Archive stroke #3 of 8 @13:48:18.43 Archive.in :pressurize @13:48:18.50 Archive.seek :pressurized @13:48:31.01 28.0psi after pressurize @13:48:31.03 Archive.in :compress @13:48:31.11 Archive.seek :empty @13:48:43.79 Reached 30.7 of 30.6psi after pumping 5148uL (2.98 strokes) @13:48:43.81 Archive.in :default @13:48:43.89 Archive.hold @13:48:43.97 Handoff.select :waste @13:48:51.67 delay 1:00 @13:49:51.68 Handoff.select :blocked @13:50:14.37 Handoff.select :waste @13:50:14.50 0.5psi at evacEnd @13:50:14.52 Air.seek :fullVent @13:50:15.96 Archive.in :fast @13:50:16.03 Archive.seek :topVent @13:50:22.99 Seawater evacuated, Pressure above/below filter released. @13:50:23.09 Lysis1.in :slow @13:50:23.17 Lysis1.seek 1050uL @13:50:53.19 delay 5 seconds @13:50:58.21 Handoff.select :blocked @13:50:58.40 Lysis1.seek 450uL @13:51:58.37 delay 3 seconds @13:52:01.45 Air.in mix with 1.40048 parts Lysis1 @13:52:01.94 Air.seek :evac @13:52:01.98 Lysis1.seek 300uL @13:52:17.12 Lysis1.seek :empty @13:52:47.22 Lysis buffer delivered @19.1psi @13:52:47.23 Polling Can every 2:00 @13:52:47.79 PuckHeater.configure :default #ambient 23.0C @13:52:47.84 PuckHeater.seek 80.7C @13:53:46.01 PuckHeater.hold 2:00,80.7C @13:55:46.10 Polling Can every 2:00 @13:55:46.64 PuckHeater.configure :default #ambient 23.0C @13:55:46.70 PuckHeater.hold 30 seconds,79.6C @13:56:16.91 Polling Can every 2:00 @13:56:17.45 PuckHeater.configure :default #ambient 23.0C @13:56:17.53 PuckHeater.hold 30 seconds,79.1C @13:56:47.77 Polling Can every 2:00 @13:56:48.33 PuckHeater.hold 10:00,78.5C @14:06:48.64 Lysis complete. Syringe pressure @21.5psi @14:06:48.67 Archive syringe pumping up to 5 strokes to reach 40psi @14:06:48.69 Archive stroke #1 of 5 @14:06:48.79 Archive.in :pressurize @14:06:48.86 Archive.seek :pressurized @14:07:01.34 21.2psi after pressurize @14:07:06.91 21.2psi after pressurize (stable) @14:07:06.93 Archive.in :compress @14:07:07.02 Archive.seek :empty @14:07:13.33 Reached 40.7 of 40.0psi after pumping 1551uL (0.90 strokes) @14:07:13.34 Archive.in :default @14:07:13.42 Archive.hold @14:07:13.50 delay 2 seconds @14:07:15.52 Archive.in :fast @14:07:15.59 Archive.seek :full @14:07:22.54 Lysis1.seek 138uL @14:07:36.41 delay 1 second @14:07:37.43 Archive.seek :full @14:07:37.47 Lysis1.seek :empty @14:07:51.38 Handoff.select :blocked @14:07:51.50 Initial air pressure @41.8psi @14:07:51.56 Lysis1.in :extract @14:07:51.69 Lysis1.seek 600uL,nil @14:07:53.92 Archive syringe pumping up to 6 strokes to reach 25psi @14:07:53.93 Archive stroke #1 of 6 @14:07:54.04 Archive.in :pressurize @14:07:54.11 Archive.seek :pressurized @14:08:06.61 13.1psi after pressurize @14:08:28.70 13.3psi after pressurize (stable) @14:08:28.72 Archive.in :compress @14:08:28.80 Archive.seek :empty @14:08:33.97 Archive.in :default @14:08:34.03 Archive.hold @14:08:34.19 Initial 600uL lysate @16.5psi @14:08:34.20 Handoff.select :blocked @14:08:34.33 Initial air pressure @16.6psi @14:08:34.40 Lysis1.seek 825uL,nil @14:08:35.61 Archive syringe pumping up to 6 strokes to reach 17psi @14:08:35.62 Archive stroke #1 of 6 @14:08:35.73 Archive.in :compress @14:08:35.80 Archive.seek :empty @14:08:41.55 Archive.in :default @14:08:41.61 Archive.hold @14:08:41.86 Recovered 825uL lysate @16.4psi @14:08:41.88 Air.in :halfSpeed @14:08:41.98 Air.seek :fullVent @14:08:44.20 Puck Top Vented, bottom @4.3psi @14:08:44.21 Handoff.select :waste @14:08:44.34 delay 5 seconds @14:08:49.42 Handoff open to waste, bottom @0.2psi @14:08:49.44 Air.in :default @14:08:49.54 Air.seek :evac @14:08:50.97 Lysis2.in :DAmix @14:08:51.09 Lysis1.in :DAmix @14:08:51.58 Lysis2.seek 1480uL @14:08:51.63 Lysis1.seek 575uL @14:09:26.71 delay 5 seconds @14:09:31.72 Handoff.select SPR @14:09:31.86 SPR.command "start 2 14" @14:09:32.96 SPR stabilized @14:09:33.11 delay 10 seconds @14:09:43.12 SPR.command "start 1 0" @14:09:45.80 Waiting up to 4:00 for SPR to enter state 1 @14:11:50.10 SPR.command "hold_on" @14:11:51.48 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:11:52.00 Lysis2.seek 1180uL @14:11:52.06 Lysis1.seek 275uL @14:12:34.07 delay 15 seconds @14:12:49.08 SPR.command "hold_off" @14:12:52.13 Waiting up to 38:00 for SPR to enter state 7 @14:23:06.03 Session Ended @14:30:51.75 SPRsummary:-43RIU,3.05RIU,12.9RIU,20.3RIU,36.5ng/L,29.7ng/L,50.5ng/L after 3191s @14:37:28.97 SPR analysis completed @14:37:34.66 Ended log spr23apr2713h.spr.bz2 @14:37:34.67 SPR.command "stats_off" @14:37:35.23 Handoff.select :close @14:37:35.35
Gate.power :heat,:OFF @14:37:35.37 Gate.power :cartridge,:OFF @14:37:35.43 PinPort.in :unlocking @14:37:35.48 PinPort.seek :clear @14:37:35.94 Carousel.in :default @14:37:36.04 Carousel.home.seek 1 @14:37:36.32 Plunge.seek :locked @14:37:37.19 Archive.in :unlocking @14:37:37.32 Archive.seek :clear @14:37:37.37 Air.in :unlocking @14:37:37.51 Air.seek :clear @14:37:37.57 Lysis1.in :unlocking @14:37:38.07 Lysis1.seek :clear @14:37:38.12 Lysis2.in :unlocking @14:37:38.22 Lysis2.seek :clear @14:37:42.16
Gate.power :motor,:OFF @14:37:42.17 Gate.power :core,:OFF @14:37:42.18 Gate.power :analytic1,:OFF @14:37:42.19 Gate.power :analytic2,:OFF @14:37:42.20 Gate.power :analytic3,:OFF @14:37:42.21 Gate.power :main,:OFF @14:37:42.22 Safely stopped and ready to power off @14:37:42.65 Cartridge::None in MAIN -- Not enough dry cartridges remain nohup: nsfmount: No such file or directory @17:03:58.48PDT01-May-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:03:58.51 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:04:01.72 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @17:04:02.29 Sea pressure (Abs) tare is 6% of full scale @17:04:10.02 Polling Can every 2:00 @17:04:10.07 Loading /home/esp/esp2/bin/processAll mission @17:04:10.27 Sea pressure (Abs) tare is 6% of full scale @17:04:10.29 Sampling default goal volumes @17:04:10.31 Options: {extraDilutions: [1,1,1]} Cartridge group: standard Spares: {spare: 0} @17:04:10.47 Gate.power :analytic1,:ON,limit to 5000mA @17:04:10.48 Gate.power :analytic2,:ON,limit to 5000mA @17:04:10.49 Gate.power :analytic3,:ON,limit to 5000mA @17:04:11.20 Archive.configure :default @17:04:11.29 SPR Module 1, FW Rev. B, FPGA Rev. C @17:04:11.40
Air.configure :default @17:04:11.52 Lysis1.configure :default @17:04:11.64 Lysis2.configure :default @17:04:11.78 Plunge.configure :default @17:04:11.95 SamplePump.configure :default @17:04:12.10 Toroid.configure :default @17:04:12.16 Toroid.hold @17:04:12.22 Toroid.configure :default @17:04:12.37 Carousel.configure :default @17:04:12.43 Carousel.hold @17:04:12.50 Carousel.configure :default @17:04:12.64 Pressure.configure :default @17:04:12.79 ExtraPump.configure :default @17:04:13.38 PinPort.configure :default @17:04:13.44 All elf controllers running firmware version 4.83 @17:04:14.23 Toroid.seek :clear @17:04:14.31 Carousel.in :default @17:04:14.43 Carousel.home.seek 35 @17:04:14.69 Selecting Cartridge 35 @17:04:15.00 Carousel.seek 35 @17:04:15.29 Lysis1.in :fast @17:04:15.35 Lysis1.seek :locked @17:04:17.98 Plunge.seek :locked @17:04:18.13 Air.in :fast @17:04:18.20 Air.seek :locked @17:04:19.77
Carousel.reconfigure :locked @17:04:19.92 Powering cartridge bus @17:04:22.56 Gate.setIR 1750,5000,0,6 @17:04:22.68 Gate.setIR 0,5000,0,6 @17:04:22.74 PuckHeater.configure :default @17:04:22.80 Gate.power :heat,:ON,limit to 5000mA @17:04:23.54 Mounted lysis Cartridge board#Q05046 @17:04:23.57 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:04:25.12 SPR.command "read_sequences" @17:04:25.39 SPR.command "stats_on" @17:04:25.54 SPR.command "start 0 1" @17:04:26.25 SPR.start /var/log/esp/spr23may0117h.spr.bz2 @17:04:28.24 Waiting for baseline SPR stabilization @17:04:28.26 Handoff.select :waste @17:04:34.97 Syringe tare is 18% of full scale @17:04:34.99 PinPort.in :locking @17:04:35.06 PinPort.seek :locked @17:04:35.57 Archive.in :fast @17:04:35.67 Lysis2.in :fast @17:04:35.72 Lysis1.seek 1000uL @17:04:35.78 Archive.seek :full @17:04:35.94 Lysis2.seek 1000uL @17:04:40.46 delayUntil "17:14:27.78" @17:14:27.80 Lysis2.in :DAmix @17:14:27.93 Lysis1.in :DAmix @17:14:28.52 Lysis2.seek 750uL @17:14:28.56 Lysis1.seek 750uL @17:15:03.69 delay 5 seconds @17:15:08.71 Handoff.select SPR @17:15:08.83 SPR.command "start 2 14" @17:15:09.51 SPR stabilized @17:15:09.66 delay 10 seconds @17:15:19.69 SPR.command "start 1 0" @17:15:22.88 Waiting up to 4:00 for SPR to enter state 1 @17:17:27.16 SPR.command "hold_on" @17:17:28.56 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:17:29.19 Lysis2.seek 450uL @17:17:29.23 Lysis1.seek 450uL @17:18:11.28 delay 15 seconds @17:18:26.30 SPR.command "hold_off" @17:18:29.21 Waiting up to 38:00 for SPR to enter state 7 @17:36:28.82 SPRsummary:-83.5RIU,60.5RIU,49.4RIU,119RIU after 2030s @17:43:06.35 delay 10 seconds @17:43:16.38 SPR.command "start 1 0" @17:43:18.89 Waiting up to 4:00 for SPR to enter state 1 @17:45:23.19 SPR.command "hold_on" @17:45:24.57 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:45:25.18 Lysis2.seek 300uL @17:45:25.24 Lysis1.seek 300uL @17:45:46.36 delay 15 seconds @17:46:01.38 SPR.command "hold_off" @17:46:04.41 Waiting up to 38:00 for SPR to enter state 7 @18:10:41.83 delay 10 seconds @18:10:51.85 SPR.command "start 1 0" @18:10:55.05 Waiting up to 4:00 for SPR to enter state 1 @18:12:59.33 SPR.command "hold_on" @18:13:00.73 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:01.30 Lysis2.seek 150uL @18:13:01.36 Lysis1.seek 150uL @18:13:22.48 delay 15 seconds @18:13:37.50 SPR.command "hold_off" @18:13:40.57 Waiting up to 38:00 for SPR to enter state 7 @18:38:17.54 delay 10 seconds @18:38:27.57 SPR.command "start 1 0" @18:38:30.26 Waiting up to 4:00 for SPR to enter state 1 @18:40:34.54 SPR.command "hold_on" @18:40:35.93 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:40:36.51 Lysis2.seek 0uL @18:40:36.57 Lysis1.seek 0uL @18:40:57.68 delay 15 seconds @18:41:12.70 SPR.command "hold_off" @18:41:15.78 Waiting up to 38:00 for SPR to enter state 7 @19:05:52.60 SPR analysis completed @19:05:54.77 Ended log spr23may0117h.spr.bz2 @19:05:54.79 SPR.command "stats_off" @19:05:55.13 Intake.close @19:05:55.22 Exhaust.close @19:05:55.27 Bypass.close @19:05:55.38 Handoff.select :close @19:05:55.56
Gate.power :heat,:OFF @19:05:55.57 Gate.power :cartridge,:OFF @19:05:55.66 PinPort.in :unlocking @19:05:55.72 PinPort.seek :clear @19:05:56.18 Carousel.in :default @19:05:56.31 Carousel.home.seek 35 @19:05:56.63 Plunge.seek :locked @19:05:56.79 Archive.in :unlocking @19:05:56.97 Archive.seek :clear @19:05:57.04 Air.in :unlocking @19:05:57.27 Air.seek :clear @19:05:57.36 Lysis1.in :unlocking @19:05:57.53 Lysis1.seek :clear @19:05:57.61 Lysis2.in :unlocking @19:05:57.71 Lysis2.seek :clear @19:06:02.30
Gate.power :motor,:OFF @19:06:02.31 Gate.power :core,:OFF @19:06:02.33 Gate.power :analytic1,:OFF @19:06:02.34 Gate.power :analytic2,:OFF @19:06:02.35 Gate.power :analytic3,:OFF @19:06:02.37 Gate.power :main,:OFF @19:06:02.38 Safely stopped and ready to power off @19:06:06.14 Gate.power :main,:ON,limit to 5000mA @19:06:06.15 Gate.power :analytic1,:ON,limit to 5000mA @19:06:06.17 Gate.power :analytic2,:ON,limit to 5000mA @19:06:06.18 Gate.power :analytic3,:ON,limit to 5000mA @19:06:06.24 Powering core bus @19:06:08.60 SPR Module 1, FW Rev. B, FPGA Rev. C @19:06:09.00
Archive.configure :default @19:06:09.12 Air.configure :default @19:06:09.19 Lysis1.configure :default @19:06:09.25 Lysis2.configure :default @19:06:09.34 Plunge.configure :default @19:06:09.43 SamplePump.configure :default @19:06:09.53 Toroid.configure :default @19:06:09.61 Carousel.configure :default @19:06:09.70 Pressure.configure :default @19:06:09.79 ExtraPump.configure :default @19:06:09.85 PinPort.configure :default @19:06:09.91 All elf controllers running firmware version 4.83 @19:06:09.93 Gate.power :motor,:ON,limit to 5000mA @19:06:10.11 Archive.home.jog 0 @19:06:10.81 Plunge.home.jog 0 @19:06:10.93 Carousel.home.jog 0 @19:06:10.97 Toroid.home.jog 0 @19:06:11.01 Lysis1.home.jog 0 @19:06:11.04 Air.home.jog 0 @19:06:11.07 PinPort.home.jog 0 @19:06:11.12 Lysis2.home.jog 0 @19:06:12.45 Archive.in :homing @19:06:12.82 Archive.jog 1377 @19:06:12.90 Toroid.in :homing @19:06:12.92 Lysis2.in :homing @19:06:12.95 Air.in :homing @19:06:12.99 PinPort.in :homing @19:06:13.06 Plunge.in :homing @19:06:13.10 Lysis1.in :homing @19:06:13.40 Lysis2.jog 1392 @19:06:13.46 Toroid.jog 611 @19:06:13.48 Air.jog 1374 @19:06:13.51 PinPort.jog -107 @19:06:13.54 Plunge.jog 1524 @19:06:13.57 Lysis1.jog 1391 @19:06:13.99 PinPort.setRawPosition! 0 @19:06:14.07 PinPort.seek :home @19:06:14.18 PinPort.in :default @19:06:14.32 Archive.setRawPosition! 50 @19:06:14.37 Archive.seek :home @19:06:14.65 Archive.in :default @19:06:14.85 Lysis2.setRawPosition! 50 @19:06:14.94 Lysis2.seek :home @19:06:14.96 Air.setRawPosition! 50 @19:06:15.07 Air.seek :home @19:06:15.19 Plunge.setRawPosition! 50 @19:06:15.22 Lysis1.setRawPosition! 50 @19:06:15.31 Plunge.seek :home @19:06:15.37 Lysis1.seek :home @19:06:15.53 Lysis2.in :default @19:06:15.56 Air.in :default @19:06:15.73 Toroid.setRawPosition! 0 @19:06:15.77 Plunge.in :default @19:06:15.80 Lysis1.in :default @19:06:15.96 Toroid.seek :home @19:06:16.06 Toroid.in :default @19:06:16.24
Toroid.seek :clear @19:06:17.85 Carousel.in :default @19:06:17.98 Carousel.home.seek 35 @19:06:18.26 Selecting Cartridge 34 @19:06:18.55 Carousel.seek 34 @19:06:19.07 Lysis1.in :fast @19:06:19.16 Lysis1.seek :locked @19:06:21.78 Plunge.seek :locked @19:06:21.94 Air.in :fast @19:06:22.00 Air.seek :locked @19:06:23.57
Carousel.reconfigure :locked @19:06:23.73 Powering cartridge bus @19:06:26.37 Gate.setIR 1750,5000,0,6 @19:06:26.50 Gate.setIR 0,5000,0,6 @19:06:26.56 PuckHeater.configure :default @19:06:26.62 Gate.power :heat,:ON,limit to 5000mA @19:06:27.81 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @19:06:27.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:06:29.39 SPR.command "read_sequences" @19:06:29.44 SPR.command "stats_on" @19:06:30.34 SPR.command "start 0 1" @19:06:31.04 SPR.start /var/log/esp/spr23may0119h.spr.bz2 @19:06:33.07 Waiting for baseline SPR stabilization @19:06:33.13 Handoff.select :waste @19:06:39.83 Syringe tare is 18% of full scale @19:06:39.85 PinPort.in :locking @19:06:39.91 PinPort.seek :locked @19:06:40.40 Archive.in :fast @19:06:40.47 Lysis2.in :fast @19:06:40.52 Lysis1.seek 1000uL @19:06:40.70 Archive.seek :full @19:06:40.77 Lysis2.seek 1000uL @19:06:45.34 delayUntil "19:16:32.64" @19:16:32.66 Lysis2.in :DAmix @19:16:32.78 Lysis1.in :DAmix @19:16:33.32 Lysis2.seek 750uL @19:16:33.40 Lysis1.seek 750uL @19:17:08.52 delay 5 seconds @19:17:13.54 Handoff.select SPR @19:17:13.67 SPR.command "start 2 14" @19:17:14.35 SPR stabilized @19:17:14.51 delay 10 seconds @19:17:24.53 SPR.command "start 1 0" @19:17:27.72 Waiting up to 4:00 for SPR to enter state 1 @19:19:32.00 SPR.command "hold_on" @19:19:33.91 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @19:19:34.50 Lysis2.seek 450uL @19:19:34.54 Lysis1.seek 450uL @19:20:16.64 delay 15 seconds @19:20:31.66 SPR.command "hold_off" @19:20:35.00 Waiting up to 38:00 for SPR to enter state 7 @19:38:33.66 SPRsummary:4.22RIU,229RIU,180RIU,299RIU after 2030s @19:45:11.02 delay 10 seconds @19:45:21.04 SPR.command "start 1 0" @19:45:24.25 Waiting up to 4:00 for SPR to enter state 1 @19:47:28.97 SPR.command "hold_on" @19:47:30.88 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:47:31.49 Lysis2.seek 300uL @19:47:31.54 Lysis1.seek 300uL @19:47:52.66 delay 15 seconds @19:48:07.69 SPR.command "hold_off" @19:48:10.20 Waiting up to 38:00 for SPR to enter state 7 @20:12:46.23 delay 10 seconds @20:12:56.25 SPR.command "start 1 0" @20:12:58.94 Waiting up to 4:00 for SPR to enter state 1 @20:15:03.23 SPR.command "hold_on" @20:15:04.62 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:15:05.20 Lysis2.seek 150uL @20:15:05.26 Lysis1.seek 150uL @20:15:26.36 delay 15 seconds @20:15:41.38 SPR.command "hold_off" @20:15:44.47 Waiting up to 38:00 for SPR to enter state 7 @20:40:21.44 delay 10 seconds @20:40:31.46 SPR.command "start 1 0" @20:40:34.16 Waiting up to 4:00 for SPR to enter state 1 @20:42:38.44 SPR.command "hold_on" @20:42:40.35 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:42:40.91 Lysis2.seek 0uL @20:42:40.96 Lysis1.seek 0uL @20:43:02.07 delay 15 seconds @20:43:17.11 SPR.command "hold_off" @20:43:20.19 Waiting up to 38:00 for SPR to enter state 7 @21:07:56.51 SPR analysis completed @21:07:58.63 Ended log spr23may0119h.spr.bz2 @21:07:58.65 SPR.command "stats_off" @21:07:59.01 Handoff.select :close @21:07:59.15
Gate.power :heat,:OFF @21:07:59.16 Gate.power :cartridge,:OFF @21:07:59.25 PinPort.in :unlocking @21:07:59.31 PinPort.seek :clear @21:07:59.78 Carousel.in :default @21:07:59.91 Carousel.home.seek 34 @21:08:00.24 Plunge.seek :locked @21:08:00.40 Archive.in :unlocking @21:08:00.56 Archive.seek :clear @21:08:00.63 Air.in :unlocking @21:08:00.80 Air.seek :clear @21:08:00.86 Lysis1.in :unlocking @21:08:01.06 Lysis1.seek :clear @21:08:01.15 Lysis2.in :unlocking @21:08:01.27 Lysis2.seek :clear @21:08:05.86
Gate.power :motor,:OFF @21:08:05.87 Gate.power :core,:OFF @21:08:05.88 Gate.power :analytic1,:OFF @21:08:05.90 Gate.power :analytic2,:OFF @21:08:05.91 Gate.power :analytic3,:OFF @21:08:05.92 Gate.power :main,:OFF @21:08:05.93 Safely stopped and ready to power off @21:08:09.69 Gate.power :main,:ON,limit to 5000mA @21:08:09.71 Gate.power :analytic1,:ON,limit to 5000mA @21:08:09.72 Gate.power :analytic2,:ON,limit to 5000mA @21:08:09.74 Gate.power :analytic3,:ON,limit to 5000mA @21:08:09.80 Powering core bus @21:08:12.17 SPR Module 1, FW Rev. B, FPGA Rev. C @21:08:12.55
Archive.configure :default @21:08:12.64 Air.configure :default @21:08:12.70 Lysis1.configure :default @21:08:12.76 Lysis2.configure :default @21:08:12.85 Plunge.configure :default @21:08:12.94 SamplePump.configure :default @21:08:13.03 Toroid.configure :default @21:08:13.15 Carousel.configure :default @21:08:13.23 Pressure.configure :default @21:08:13.32 ExtraPump.configure :default @21:08:13.38 PinPort.configure :default @21:08:13.44 All elf controllers running firmware version 4.83 @21:08:13.46 Gate.power :motor,:ON,limit to 5000mA @21:08:13.68 Archive.home.jog 0 @21:08:14.44 PinPort.home.jog 0 @21:08:14.47 Carousel.home.jog 0 @21:08:14.50 Toroid.home.jog 0 @21:08:14.54 Lysis2.home.jog 0 @21:08:14.57 Lysis1.home.jog 0 @21:08:14.61 Plunge.home.jog 0 @21:08:14.65 Air.home.jog 0 @21:08:16.13 Archive.in :homing @21:08:16.35 Archive.jog 1374 @21:08:16.42 Toroid.in :homing @21:08:16.45 Plunge.in :homing @21:08:16.49 Lysis2.in :homing @21:08:16.54 Air.in :homing @21:08:16.58 Lysis1.in :homing @21:08:16.63 PinPort.in :homing @21:08:16.94 Toroid.jog 614 @21:08:16.99 Plunge.jog 1516 @21:08:17.02 Lysis2.jog 1395 @21:08:17.05 Lysis1.jog 1395 @21:08:17.09 Air.jog 1375 @21:08:17.17 PinPort.jog -107 @21:08:17.64 PinPort.setRawPosition! 0 @21:08:17.69 PinPort.seek :home @21:08:17.77 PinPort.in :default @21:08:17.90 Archive.setRawPosition! 50 @21:08:17.95 Archive.seek :home @21:08:18.23 Archive.in :default @21:08:18.42 Lysis2.setRawPosition! 50 @21:08:18.50 Plunge.setRawPosition! 50 @21:08:18.56 Lysis2.seek :home @21:08:18.61 Plunge.seek :home @21:08:18.77 Lysis1.setRawPosition! 50 @21:08:18.80 Air.setRawPosition! 50 @21:08:18.88 Lysis1.seek :home @21:08:18.95 Air.seek :home @21:08:19.13 Lysis2.in :default @21:08:19.17 Plunge.in :default @21:08:19.35 Toroid.setRawPosition! 0 @21:08:19.39 Lysis1.in :default @21:08:19.41 Air.in :default @21:08:19.54 Toroid.seek :home @21:08:19.70 Toroid.in :default @21:08:19.91
Toroid.seek :clear @21:08:21.52 Carousel.in :default @21:08:21.64 Carousel.home.seek 34 @21:08:21.94 Selecting Cartridge 33 @21:08:22.24 Carousel.seek 33 @21:08:22.75 Lysis1.in :fast @21:08:22.81 Lysis1.seek :locked @21:08:25.44 Plunge.seek :locked @21:08:25.60 Air.in :fast @21:08:25.66 Air.seek :locked @21:08:27.25
Carousel.reconfigure :locked @21:08:27.41 Powering cartridge bus @21:08:30.05 Gate.setIR 1750,5000,0,6 @21:08:30.17 Gate.setIR 0,5000,0,6 @21:08:30.23 PuckHeater.configure :default @21:08:30.76 Gate.power :heat,:ON,limit to 5000mA @21:08:31.46 Mounted lysis Cartridge board#Q05049 @21:08:31.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:08:33.04 SPR.command "read_sequences" @21:08:33.79 SPR.command "stats_on" @21:08:33.95 SPR.command "start 0 1" @21:08:34.65 SPR.start /var/log/esp/spr23may0121h.spr.bz2 @21:08:37.17 Waiting for baseline SPR stabilization @21:08:37.19 Handoff.select :waste @21:08:43.88 Syringe tare is 18% of full scale @21:08:43.91 PinPort.in :locking @21:08:43.97 PinPort.seek :locked @21:08:44.47 Archive.in :fast @21:08:44.53 Lysis2.in :fast @21:08:44.63 Lysis1.seek 1000uL @21:08:44.78 Archive.seek :full @21:08:44.85 Lysis2.seek 1000uL @21:08:49.41 delayUntil "21:18:37.14" @21:18:37.15 Lysis2.in :DAmix @21:18:37.28 Lysis1.in :DAmix @21:18:37.82 Lysis2.seek 750uL @21:18:37.85 Lysis1.seek 750uL @21:19:12.98 delay 5 seconds @21:19:18.00 Handoff.select SPR @21:19:18.13 SPR.command "start 2 14" @21:19:18.86 SPR stabilized @21:19:19.01 delay 10 seconds @21:19:29.03 SPR.command "start 1 0" @21:19:32.22 Waiting up to 4:00 for SPR to enter state 1 @21:21:36.51 SPR.command "hold_on" @21:21:37.90 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:21:38.51 Lysis2.seek 450uL @21:21:38.56 Lysis1.seek 450uL @21:22:20.63 delay 15 seconds @21:22:35.65 SPR.command "hold_off" @21:22:38.55 Waiting up to 38:00 for SPR to enter state 7 @21:40:38.17 SPRsummary:5.01RIU,221RIU,180RIU,294RIU after 2031s @21:47:15.53 delay 10 seconds @21:47:25.55 SPR.command "start 1 0" @21:47:28.24 Waiting up to 4:00 for SPR to enter state 1 @21:49:32.52 SPR.command "hold_on" @21:49:33.92 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @21:49:34.50 Lysis2.seek 300uL @21:49:34.57 Lysis1.seek 300uL @21:49:55.67 delay 15 seconds @21:50:10.69 SPR.command "hold_off" @21:50:14.28 Waiting up to 38:00 for SPR to enter state 7 @22:14:51.69 delay 10 seconds @22:15:01.71 SPR.command "start 1 0" @22:15:04.40 Waiting up to 4:00 for SPR to enter state 1 @22:17:08.69 SPR.command "hold_on" @22:17:10.60 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @22:17:11.15 Lysis2.seek 150uL @22:17:11.20 Lysis1.seek 150uL @22:17:32.32 delay 15 seconds @22:17:47.36 SPR.command "hold_off" @22:17:49.92 Waiting up to 38:00 for SPR to enter state 7 @22:42:25.95 delay 10 seconds @22:42:35.97 SPR.command "start 1 0" @22:42:38.66 Waiting up to 4:00 for SPR to enter state 1 @22:44:42.96 SPR.command "hold_on" @22:44:44.34 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @22:44:44.89 Lysis2.seek 0uL @22:44:44.96 Lysis1.seek 0uL @22:45:06.09 delay 15 seconds @22:45:21.11 SPR.command "hold_off" @22:45:24.18 Waiting up to 38:00 for SPR to enter state 7 @23:10:01.02 SPR analysis completed @23:10:03.13 Ended log spr23may0121h.spr.bz2 @23:10:03.16 SPR.command "stats_off" @23:10:03.52 Handoff.select :close @23:10:03.66
Gate.power :heat,:OFF @23:10:03.67 Gate.power :cartridge,:OFF @23:10:03.75 PinPort.in :unlocking @23:10:04.25 PinPort.seek :clear @23:10:04.72 Carousel.in :default @23:10:04.84 Carousel.home.seek 33 @23:10:05.20 Plunge.seek :locked @23:10:05.36 Archive.in :unlocking @23:10:05.51 Archive.seek :clear @23:10:05.62 Air.in :unlocking @23:10:05.76 Air.seek :clear @23:10:05.84 Lysis1.in :unlocking @23:10:06.02 Lysis1.seek :clear @23:10:06.08 Lysis2.in :unlocking @23:10:06.20 Lysis2.seek :clear @23:10:10.78
Gate.power :motor,:OFF @23:10:10.80 Gate.power :core,:OFF @23:10:10.81 Gate.power :analytic1,:OFF @23:10:10.82 Gate.power :analytic2,:OFF @23:10:10.84 Gate.power :analytic3,:OFF @23:10:10.85 Gate.power :main,:OFF @23:10:10.86 Safely stopped and ready to power off @23:10:14.62 Gate.power :main,:ON,limit to 5000mA @23:10:14.64 Gate.power :analytic1,:ON,limit to 5000mA @23:10:14.65 Gate.power :analytic2,:ON,limit to 5000mA @23:10:14.67 Gate.power :analytic3,:ON,limit to 5000mA @23:10:14.73 Powering core bus @23:10:17.09 SPR Module 1, FW Rev. B, FPGA Rev. C @23:10:17.48
Archive.configure :default @23:10:17.57 Air.configure :default @23:10:17.64 Lysis1.configure :default @23:10:17.70 Lysis2.configure :default @23:10:17.79 Plunge.configure :default @23:10:17.89 SamplePump.configure :default @23:10:17.98 Toroid.configure :default @23:10:18.06 Carousel.configure :default @23:10:18.15 Pressure.configure :default @23:10:18.24 ExtraPump.configure :default @23:10:18.30 PinPort.configure :default @23:10:18.37 All elf controllers running firmware version 4.83 @23:10:18.39 Gate.power :motor,:ON,limit to 5000mA @23:10:18.63 Archive.home.jog 0 @23:10:19.41 PinPort.home.jog 0 @23:10:19.45 Toroid.home.jog 0 @23:10:19.50 Plunge.home.jog 0 @23:10:19.53 Lysis1.home.jog 0 @23:10:19.56 Air.home.jog 0 @23:10:19.59 Carousel.home.jog 0 @23:10:19.63 Lysis2.home.jog 0 @23:10:20.37 Archive.in :homing @23:10:20.66 Toroid.in :homing @23:10:20.77 Archive.jog 1372 @23:10:20.82 Lysis2.in :homing @23:10:20.85 Air.in :homing @23:10:21.35 Lysis1.in :homing @23:10:21.49 PinPort.in :homing @23:10:21.52 Plunge.in :homing @23:10:21.83 Toroid.jog 620 @23:10:21.88 Lysis2.jog 1397 @23:10:21.91 PinPort.jog -107 @23:10:21.94 Air.jog 1372 @23:10:22.02 Plunge.jog 1518 @23:10:22.05 Lysis1.jog 1394 @23:10:22.38 PinPort.setRawPosition! 0 @23:10:22.43 PinPort.seek :home @23:10:22.53 PinPort.in :default @23:10:22.77 Archive.setRawPosition! 50 @23:10:22.83 Archive.seek :home @23:10:23.11 Archive.in :default @23:10:23.25 Lysis2.setRawPosition! 50 @23:10:23.30 Lysis2.seek :home @23:10:23.40 Air.setRawPosition! 50 @23:10:23.45 Air.seek :home @23:10:23.53 Plunge.setRawPosition! 50 @23:10:23.58 Lysis1.setRawPosition! 50 @23:10:23.66 Plunge.seek :home @23:10:23.75 Lysis1.seek :home @23:10:23.89 Air.in :default @23:10:23.92 Lysis2.in :default @23:10:24.09 Plunge.in :default @23:10:24.13 Lysis1.in :default @23:10:24.16 Toroid.setRawPosition! 0 @23:10:24.31 Toroid.seek :home @23:10:24.39 Toroid.in :default @23:10:24.55
Toroid.seek :clear @23:10:26.16 Carousel.in :default @23:10:26.29 Carousel.home.seek 33 @23:10:26.58 Selecting Cartridge 32 @23:10:26.89 Carousel.seek 32 @23:10:27.42 Lysis1.in :fast @23:10:27.48 Lysis1.seek :locked @23:10:30.11 Plunge.seek :locked @23:10:30.27 Air.in :fast @23:10:30.33 Air.seek :locked @23:10:31.90
Carousel.reconfigure :locked @23:10:32.05 Powering cartridge bus @23:10:34.70 Gate.setIR 1750,5000,0,6 @23:10:34.82 Gate.setIR 0,5000,0,6 @23:10:34.88 PuckHeater.configure :default @23:10:34.94 Gate.power :heat,:ON,limit to 5000mA @23:10:35.62 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05040 @23:10:36.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:10:37.69 SPR.command "read_sequences" @23:10:37.77 SPR.command "stats_on" @23:10:37.93 SPR.command "start 0 1" @23:10:38.63 SPR.start /var/log/esp/spr23may0123h.spr.bz2 @23:10:41.17 Waiting for baseline SPR stabilization @23:10:41.18 Handoff.select :waste @23:10:47.88 Syringe tare is 18% of full scale @23:10:47.91 PinPort.in :locking @23:10:47.97 PinPort.seek :locked @23:10:48.47 Archive.in :fast @23:10:48.54 Lysis2.in :fast @23:10:48.61 Lysis1.seek 1000uL @23:10:48.85 Archive.seek :full @23:10:48.90 Lysis2.seek 1000uL @23:10:53.48 delayUntil "23:20:41.12" @23:20:41.14 Lysis2.in :DAmix @23:20:41.27 Lysis1.in :DAmix @23:20:41.78 Lysis2.seek 750uL @23:20:41.83 Lysis1.seek 750uL @23:21:16.99 delay 5 seconds @23:21:22.01 Handoff.select SPR @23:21:22.13 SPR.command "start 2 14" @23:21:23.36 SPR stabilized @23:21:23.51 delay 10 seconds @23:21:33.53 SPR.command "start 1 0" @23:21:36.21 Waiting up to 4:00 for SPR to enter state 1 @23:23:40.50 SPR.command "hold_on" @23:23:41.89 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @23:23:42.51 Lysis2.seek 450uL @23:23:42.54 Lysis1.seek 450uL @23:24:24.62 delay 15 seconds @23:24:39.64 SPR.command "hold_off" @23:24:42.54 Waiting up to 38:00 for SPR to enter state 7 @23:42:42.15 SPRsummary:2.83RIU,202RIU,163RIU,277RIU after 2031s @23:49:19.52 delay 10 seconds @23:49:29.54 SPR.command "start 1 0" @23:49:32.23 Waiting up to 4:00 for SPR to enter state 1 @23:51:36.52 SPR.command "hold_on" @23:51:37.90 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @23:51:38.50 Lysis2.seek 300uL @23:51:38.56 Lysis1.seek 300uL @23:51:59.66 delay 15 seconds @23:52:14.68 SPR.command "hold_off" @23:52:17.75 Waiting up to 38:00 for SPR to enter state 7 @00:16:54.72PDT02-May-23 delay 10 seconds @00:17:04.74 SPR.command "start 1 0" @00:17:07.44 Waiting up to 4:00 for SPR to enter state 1 @00:19:11.72 SPR.command "hold_on" @00:19:13.14 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @00:19:13.68 Lysis2.seek 150uL @00:19:13.74 Lysis1.seek 150uL @00:19:34.85 delay 15 seconds @00:19:49.87 SPR.command "hold_off" @00:19:52.96 Waiting up to 38:00 for SPR to enter state 7 @00:44:29.93 delay 10 seconds @00:44:39.95 SPR.command "start 1 0" @00:44:43.18 Waiting up to 4:00 for SPR to enter state 1 @00:46:47.88 SPR.command "hold_on" @00:46:49.27 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @00:46:49.87 Lysis2.seek 0uL @00:46:49.90 Lysis1.seek 0uL @00:47:11.02 delay 15 seconds @00:47:26.04 SPR.command "hold_off" @00:47:29.63 Waiting up to 38:00 for SPR to enter state 7 @01:12:06.90 SPR analysis completed @01:12:09.08 Ended log spr23may0123h.spr.bz2 @01:12:09.09 SPR.command "stats_off" @01:12:10.52 Handoff.select :close @01:12:10.67
Gate.power :heat,:OFF @01:12:10.69 Gate.power :cartridge,:OFF @01:12:10.77 PinPort.in :unlocking @01:12:10.83 PinPort.seek :clear @01:12:11.30 Carousel.in :default @01:12:11.43 Carousel.home.seek 32 @01:12:11.76 Plunge.seek :locked @01:12:11.93 Archive.in :unlocking @01:12:12.09 Archive.seek :clear @01:12:12.17 Air.in :unlocking @01:12:12.33 Air.seek :clear @01:12:12.40 Lysis1.in :unlocking @01:12:12.56 Lysis1.seek :clear @01:12:12.66 Lysis2.in :unlocking @01:12:12.74 Lysis2.seek :clear @01:12:17.32
Gate.power :motor,:OFF @01:12:17.33 Gate.power :core,:OFF @01:12:17.35 Gate.power :analytic1,:OFF @01:12:17.36 Gate.power :analytic2,:OFF @01:12:17.38 Gate.power :analytic3,:OFF @01:12:17.39 Gate.power :main,:OFF @01:12:17.40 Safely stopped and ready to power off @01:12:21.16 Gate.power :main,:ON,limit to 5000mA @01:12:21.17 Gate.power :analytic1,:ON,limit to 5000mA @01:12:21.19 Gate.power :analytic2,:ON,limit to 5000mA @01:12:21.20 Gate.power :analytic3,:ON,limit to 5000mA @01:12:21.27 Powering core bus @01:12:23.63 SPR Module 1, FW Rev. B, FPGA Rev. C @01:12:24.02
Archive.configure :default @01:12:24.11 Air.configure :default @01:12:24.17 Lysis1.configure :default @01:12:24.24 Lysis2.configure :default @01:12:24.32 Plunge.configure :default @01:12:24.41 SamplePump.configure :default @01:12:24.50 Toroid.configure :default @01:12:24.59 Carousel.configure :default @01:12:24.68 Pressure.configure :default @01:12:24.77 ExtraPump.configure :default @01:12:24.83 PinPort.configure :default @01:12:24.89 All elf controllers running firmware version 4.83 @01:12:24.91 Gate.power :motor,:ON,limit to 5000mA @01:12:25.19 Archive.home.jog 0 @01:12:25.93 Carousel.home.jog 0 @01:12:25.99 Toroid.home.jog 0 @01:12:26.01 Lysis2.home.jog 0 @01:12:26.04 Lysis1.home.jog 0 @01:12:26.07 Plunge.home.jog 0 @01:12:26.10 Air.home.jog 0 @01:12:26.14 PinPort.home.jog 0 @01:12:27.59 Archive.in :homing @01:12:27.85 Archive.jog 1376 @01:12:27.91 PinPort.in :homing @01:12:27.94 Toroid.in :homing @01:12:27.98 Plunge.in :homing @01:12:28.03 Lysis1.in :homing @01:12:28.07 Lysis2.in :homing @01:12:28.12 Air.in :homing @01:12:28.38 PinPort.jog -108 @01:12:28.47 Plunge.jog 1519 @01:12:28.56 Toroid.jog 617 @01:12:28.58 Lysis2.jog 1395 @01:12:28.63 Lysis1.jog 1393 @01:12:28.66 Air.jog 1375 @01:12:28.98 PinPort.setRawPosition! 0 @01:12:29.03 PinPort.seek :home @01:12:29.15 PinPort.in :default @01:12:29.35 Archive.setRawPosition! 50 @01:12:29.40 Archive.seek :home @01:12:29.68 Archive.in :default @01:12:29.91 Lysis2.setRawPosition! 50 @01:12:30.00 Plunge.setRawPosition! 50 @01:12:30.02 Lysis2.seek :home @01:12:30.12 Plunge.seek :home @01:12:30.22 Lysis1.setRawPosition! 50 @01:12:30.26 Air.setRawPosition! 50 @01:12:30.34 Lysis1.seek :home @01:12:30.43 Air.seek :home @01:12:30.56 Plunge.in :default @01:12:30.60 Lysis2.in :default @01:12:30.77 Lysis1.in :default @01:12:30.80 Air.in :default @01:12:30.94 Toroid.setRawPosition! 0 @01:12:30.99 Toroid.seek :home @01:12:31.08 Toroid.in :default @01:12:31.23
Toroid.seek :clear @01:12:32.85 Carousel.in :default @01:12:32.97 Carousel.home.seek 32 @01:12:33.26 Selecting Cartridge 31 @01:12:33.57 Carousel.seek 31 @01:12:34.08 Lysis1.in :fast @01:12:34.14 Lysis1.seek :locked @01:12:36.78 Plunge.seek :locked @01:12:36.94 Air.in :fast @01:12:37.50 Air.seek :locked @01:12:39.08
Carousel.reconfigure :locked @01:12:39.27 Powering cartridge bus @01:12:41.91 Gate.setIR 1750,5000,0,6 @01:12:42.03 Gate.setIR 0,5000,0,6 @01:12:42.10 PuckHeater.configure :default @01:12:42.16 Gate.power :heat,:ON,limit to 5000mA @01:12:42.82 Mounted lysis Cartridge board#Q05080 @01:12:42.86 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:12:44.48 SPR.command "read_sequences" @01:12:45.25 SPR.command "stats_on" @01:12:45.41 SPR.command "start 0 1" @01:12:46.11 SPR.start /var/log/esp/spr23may0201h.spr.bz2 @01:12:48.63 Waiting for baseline SPR stabilization @01:12:48.65 Handoff.select :waste @01:12:55.35 Syringe tare is 18% of full scale @01:12:55.37 PinPort.in :locking @01:12:55.44 PinPort.seek :locked @01:12:55.92 Archive.in :fast @01:12:56.01 Lysis2.in :fast @01:12:56.09 Lysis1.seek 1000uL @01:12:56.22 Archive.seek :full @01:12:56.35 Lysis2.seek 1000uL @01:13:00.90 delayUntil "01:22:48.59" @01:22:48.61 Lysis2.in :DAmix @01:22:48.73 Lysis1.in :DAmix @01:22:49.31 Lysis2.seek 750uL @01:22:49.36 Lysis1.seek 750uL @01:23:24.52 delay 5 seconds @01:23:29.54 Handoff.select SPR @01:23:29.67 SPR.command "start 2 14" @01:23:30.33 SPR stabilized @01:23:30.48 delay 10 seconds @01:23:40.50 SPR.command "start 1 0" @01:23:43.69 Waiting up to 4:00 for SPR to enter state 1 @01:25:47.99 SPR.command "hold_on" @01:25:49.37 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:25:49.97 Lysis2.seek 450uL @01:25:50.03 Lysis1.seek 450uL @01:26:32.11 delay 15 seconds @01:26:47.13 SPR.command "hold_off" @01:26:50.02 Waiting up to 38:00 for SPR to enter state 7 @01:44:49.64 SPRsummary:2.66RIU,117RIU,110RIU,181RIU after 2031s @01:51:27.01 delay 10 seconds @01:51:37.03 SPR.command "start 1 0" @01:51:39.71 Waiting up to 4:00 for SPR to enter state 1 @01:53:44.01 SPR.command "hold_on" @01:53:45.39 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @01:53:46.00 Lysis2.seek 300uL @01:53:46.06 Lysis1.seek 300uL @01:54:07.19 delay 15 seconds @01:54:22.21 SPR.command "hold_off" @01:54:25.23 Waiting up to 38:00 for SPR to enter state 7 @02:19:02.21 delay 10 seconds @02:19:12.23 SPR.command "start 1 0" @02:19:15.44 Waiting up to 4:00 for SPR to enter state 1 @02:21:20.16 SPR.command "hold_on" @02:21:22.06 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:22.62 Lysis2.seek 150uL @02:21:22.66 Lysis1.seek 150uL @02:21:43.80 delay 15 seconds @02:21:58.83 SPR.command "hold_off" @02:22:01.91 Waiting up to 38:00 for SPR to enter state 7 @02:46:38.36 delay 10 seconds @02:46:48.39 SPR.command "start 1 0" @02:46:51.08 Waiting up to 4:00 for SPR to enter state 1 @02:48:55.36 SPR.command "hold_on" @02:48:57.27 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:48:57.84 Lysis2.seek 0uL @02:48:57.89 Lysis1.seek 0uL @02:49:19.01 delay 15 seconds @02:49:34.05 SPR.command "hold_off" @02:49:36.60 Waiting up to 38:00 for SPR to enter state 7 @03:14:12.49 SPR analysis completed @03:14:14.67 Ended log spr23may0201h.spr.bz2 @03:14:14.68 SPR.command "stats_off" @03:14:15.57 Handoff.select :close @03:14:15.72
Gate.power :heat,:OFF @03:14:15.73 Gate.power :cartridge,:OFF @03:14:15.81 PinPort.in :unlocking @03:14:15.88 PinPort.seek :clear @03:14:16.35 Carousel.in :default @03:14:16.48 Carousel.home.seek 31 @03:14:16.81 Plunge.seek :locked @03:14:16.97 Archive.in :unlocking @03:14:17.16 Archive.seek :clear @03:14:17.23 Air.in :unlocking @03:14:17.41 Air.seek :clear @03:14:17.52 Lysis1.in :unlocking @03:14:17.67 Lysis1.seek :clear @03:14:17.73 Lysis2.in :unlocking @03:14:17.84 Lysis2.seek :clear @03:14:22.44
Gate.power :motor,:OFF @03:14:22.45 Gate.power :core,:OFF @03:14:22.46 Gate.power :analytic1,:OFF @03:14:22.48 Gate.power :analytic2,:OFF @03:14:22.49 Gate.power :analytic3,:OFF @03:14:22.50 Gate.power :main,:OFF @03:14:22.52 Safely stopped and ready to power off @03:14:26.27 Gate.power :main,:ON,limit to 5000mA @03:14:26.29 Gate.power :analytic1,:ON,limit to 5000mA @03:14:26.31 Gate.power :analytic2,:ON,limit to 5000mA @03:14:26.32 Gate.power :analytic3,:ON,limit to 5000mA @03:14:26.39 Powering core bus @03:14:28.75 SPR Module 1, FW Rev. B, FPGA Rev. C @03:14:29.17
Archive.configure :default @03:14:29.26 Air.configure :default @03:14:29.33 Lysis1.configure :default @03:14:29.39 Lysis2.configure :default @03:14:29.48 Plunge.configure :default @03:14:29.57 SamplePump.configure :default @03:14:29.66 Toroid.configure :default @03:14:29.75 Carousel.configure :default @03:14:29.84 Pressure.configure :default @03:14:29.93 ExtraPump.configure :default @03:14:29.99 PinPort.configure :default @03:14:30.05 All elf controllers running firmware version 4.83 @03:14:30.07 Gate.power :motor,:ON,limit to 5000mA @03:14:30.35 Archive.home.jog 0 @03:14:31.07 PinPort.home.jog 0 @03:14:31.14 Toroid.home.jog 0 @03:14:31.19 Plunge.home.jog 0 @03:14:31.22 Lysis1.home.jog 0 @03:14:31.24 Air.home.jog 0 @03:14:31.28 Carousel.home.jog 0 @03:14:31.33 Lysis2.home.jog 0 @03:14:32.56 Archive.in :homing @03:14:32.92 Archive.jog 1375 @03:14:32.99 PinPort.in :homing @03:14:33.03 Plunge.in :homing @03:14:33.06 Lysis1.in :homing @03:14:33.14 Toroid.in :homing @03:14:33.18 Air.in :homing @03:14:33.25 Lysis2.in :homing @03:14:33.50 Plunge.jog 1525 @03:14:33.53 PinPort.jog -108 @03:14:33.60 Lysis1.jog 1395 @03:14:33.65 Toroid.jog 615 @03:14:33.74 Air.jog 1373 @03:14:33.78 Lysis2.jog 1396 @03:14:34.09 PinPort.setRawPosition! 0 @03:14:34.14 PinPort.seek :home @03:14:34.24 PinPort.in :default @03:14:34.37 Archive.setRawPosition! 50 @03:14:34.43 Archive.seek :home @03:14:34.70 Archive.in :default @03:14:34.90 Plunge.setRawPosition! 50 @03:14:34.95 Plunge.seek :home @03:14:35.03 Lysis1.setRawPosition! 50 @03:14:35.09 Lysis1.seek :home @03:14:35.21 Lysis2.setRawPosition! 50 @03:14:35.25 Air.setRawPosition! 50 @03:14:35.35 Lysis2.seek :home @03:14:35.39 Air.seek :home @03:14:35.54 Lysis1.in :default @03:14:35.57 Plunge.in :default @03:14:35.75 Air.in :default @03:14:35.78 Lysis2.in :default @03:14:35.97 Toroid.setRawPosition! 0 @03:14:36.02 Toroid.seek :home @03:14:36.11 Toroid.in :default @03:14:36.21
Toroid.seek :clear @03:14:37.82 Carousel.in :default @03:14:37.94 Carousel.home.seek 31 @03:14:38.21 Selecting Cartridge 30 @03:14:38.51 Carousel.seek 30 @03:14:39.02 Lysis1.in :fast @03:14:39.08 Lysis1.seek :locked @03:14:41.73 Plunge.seek :locked @03:14:41.89 Air.in :fast @03:14:41.95 Air.seek :locked @03:14:43.52
Carousel.reconfigure :locked @03:14:44.17 Powering cartridge bus @03:14:46.81 Gate.setIR 1750,5000,0,6 @03:14:46.93 Gate.setIR 0,5000,0,6 @03:14:47.00 PuckHeater.configure :default @03:14:47.06 Gate.power :heat,:ON,limit to 5000mA @03:14:47.73 Mounted lysis Cartridge board#Q05060 @03:14:47.76 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:14:49.30 SPR.command "read_sequences" @03:14:49.42 SPR.command "stats_on" @03:14:49.58 SPR.command "start 0 1" @03:14:50.28 SPR.start /var/log/esp/spr23may0203h.spr.bz2 @03:14:52.27 Waiting for baseline SPR stabilization @03:14:52.30 Handoff.select :waste @03:14:58.99 Syringe tare is 18% of full scale @03:14:59.02 PinPort.in :locking @03:14:59.08 PinPort.seek :locked @03:14:59.60 Archive.in :fast @03:14:59.67 Lysis2.in :fast @03:14:59.74 Lysis1.seek 1000uL @03:14:59.92 Lysis2.seek 1000uL @03:14:59.98 Archive.seek :full @03:15:04.53 delayUntil "03:24:51.84" @03:24:51.85 Lysis2.in :DAmix @03:24:51.98 Lysis1.in :DAmix @03:24:52.52 Lysis2.seek 750uL @03:24:52.57 Lysis1.seek 750uL @03:25:27.70 delay 5 seconds @03:25:32.72 Handoff.select SPR @03:25:32.85 SPR.command "start 2 14" @03:25:34.07 SPR stabilized @03:25:34.22 delay 10 seconds @03:25:44.25 SPR.command "start 1 0" @03:25:46.92 Waiting up to 4:00 for SPR to enter state 1 @03:27:51.21 SPR.command "hold_on" @03:27:52.59 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:27:53.24 Lysis2.seek 450uL @03:27:53.31 Lysis1.seek 450uL @03:28:35.38 delay 15 seconds @03:28:50.40 SPR.command "hold_off" @03:28:53.25 Waiting up to 38:00 for SPR to enter state 7 @03:46:52.86 SPRsummary:3.22RIU,74.3RIU,73RIU,115RIU after 2030s @03:53:30.23 delay 10 seconds @03:53:40.25 SPR.command "start 1 0" @03:53:42.93 Waiting up to 4:00 for SPR to enter state 1 @03:55:47.23 SPR.command "hold_on" @03:55:48.61 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:55:49.25 Lysis2.seek 300uL @03:55:49.31 Lysis1.seek 300uL @03:56:10.42 delay 15 seconds @03:56:25.44 SPR.command "hold_off" @03:56:28.45 Waiting up to 38:00 for SPR to enter state 7 @04:21:05.89 delay 10 seconds @04:21:15.91 SPR.command "start 1 0" @04:21:19.09 Waiting up to 4:00 for SPR to enter state 1 @04:23:23.38 SPR.command "hold_on" @04:23:24.77 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:25.38 Lysis2.seek 150uL @04:23:25.41 Lysis1.seek 150uL @04:23:46.50 delay 15 seconds @04:24:01.52 SPR.command "hold_off" @04:24:04.68 Waiting up to 38:00 for SPR to enter state 7 @04:48:41.59 delay 10 seconds @04:48:51.61 SPR.command "start 1 0" @04:48:54.81 Waiting up to 4:00 for SPR to enter state 1 @04:50:59.60 SPR.command "hold_on" @04:51:01.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:01.97 Lysis2.seek 0uL @04:51:02.02 Lysis1.seek 0uL @04:51:23.14 delay 15 seconds @04:51:38.18 SPR.command "hold_off" @04:51:40.76 Waiting up to 38:00 for SPR to enter state 7 @05:16:16.64 SPR analysis completed @05:16:18.74 Ended log spr23may0203h.spr.bz2 @05:16:18.76 SPR.command "stats_off" @05:16:19.62 Handoff.select :close @05:16:19.76
Gate.power :heat,:OFF @05:16:19.77 Gate.power :cartridge,:OFF @05:16:19.86 PinPort.in :unlocking @05:16:19.92 PinPort.seek :clear @05:16:20.38 Carousel.in :default @05:16:20.51 Carousel.home.seek 30 @05:16:20.85 Plunge.seek :locked @05:16:21.01 Archive.in :unlocking @05:16:21.19 Archive.seek :clear @05:16:21.27 Air.in :unlocking @05:16:21.45 Air.seek :clear @05:16:21.52 Lysis1.in :unlocking @05:16:21.69 Lysis1.seek :clear @05:16:21.79 Lysis2.in :unlocking @05:16:21.86 Lysis2.seek :clear @05:16:26.45
Gate.power :motor,:OFF @05:16:26.46 Gate.power :core,:OFF @05:16:26.47 Gate.power :analytic1,:OFF @05:16:26.49 Gate.power :analytic2,:OFF @05:16:26.50 Gate.power :analytic3,:OFF @05:16:26.51 Gate.power :main,:OFF @05:16:26.53 Safely stopped and ready to power off @05:16:30.28 Gate.power :main,:ON,limit to 5000mA @05:16:30.30 Gate.power :analytic1,:ON,limit to 5000mA @05:16:30.32 Gate.power :analytic2,:ON,limit to 5000mA @05:16:30.33 Gate.power :analytic3,:ON,limit to 5000mA @05:16:30.40 Powering core bus @05:16:32.76 SPR Module 1, FW Rev. B, FPGA Rev. C @05:16:33.18
Archive.configure :default @05:16:33.28 Air.configure :default @05:16:33.35 Lysis1.configure :default @05:16:33.41 Lysis2.configure :default @05:16:33.50 Plunge.configure :default @05:16:33.59 SamplePump.configure :default @05:16:33.68 Toroid.configure :default @05:16:33.77 Carousel.configure :default @05:16:33.86 Pressure.configure :default @05:16:33.95 ExtraPump.configure :default @05:16:34.01 PinPort.configure :default @05:16:34.07 All elf controllers running firmware version 4.83 @05:16:34.10 Gate.power :motor,:ON,limit to 5000mA @05:16:34.27 Archive.home.jog 0 @05:16:35.08 PinPort.home.jog 0 @05:16:35.15 Toroid.home.jog 0 @05:16:35.18 Plunge.home.jog 0 @05:16:35.22 Lysis1.home.jog 0 @05:16:35.24 Air.home.jog 0 @05:16:35.30 Carousel.home.jog 0 @05:16:35.33 Lysis2.home.jog 0 @05:16:36.45 Archive.in :homing @05:16:36.78 Archive.jog 1373 @05:16:37.01 Toroid.in :homing @05:16:37.07 Plunge.in :homing @05:16:37.13 Lysis1.in :homing @05:16:37.18 PinPort.in :homing @05:16:37.24 Lysis2.in :homing @05:16:37.27 Air.in :homing @05:16:37.51 Toroid.jog 618 @05:16:37.58 Lysis1.jog 1390 @05:16:37.62 PinPort.jog -108 @05:16:37.65 Plunge.jog 1524 @05:16:37.68 Air.jog 1371 @05:16:37.71 Lysis2.jog 1394 @05:16:38.13 PinPort.setRawPosition! 0 @05:16:38.16 Archive.setRawPosition! 50 @05:16:38.26 PinPort.seek :home @05:16:38.29 Archive.seek :home @05:16:38.48 PinPort.in :default @05:16:38.65 Archive.in :default @05:16:39.05 Lysis1.setRawPosition! 50 @05:16:39.17 Plunge.setRawPosition! 50 @05:16:39.21 Lysis1.seek :home @05:16:39.23 Air.setRawPosition! 50 @05:16:39.35 Plunge.seek :home @05:16:39.46 Air.seek :home @05:16:39.57 Lysis2.setRawPosition! 50 @05:16:39.65 Lysis1.in :default @05:16:39.69 Lysis2.seek :home @05:16:39.87 Toroid.setRawPosition! 0 @05:16:39.91 Plunge.in :default @05:16:39.94 Air.in :default @05:16:40.07 Toroid.seek :home @05:16:40.21 Lysis2.in :default @05:16:40.32 Toroid.in :default @05:16:40.58
Toroid.seek :clear @05:16:42.19 Carousel.in :default @05:16:42.32 Carousel.home.seek 30 @05:16:42.58 Selecting Cartridge 29 @05:16:42.89 Carousel.seek 29 @05:16:43.40 Lysis1.in :fast @05:16:43.46 Lysis1.seek :locked @05:16:46.08 Plunge.seek :locked @05:16:46.24 Air.in :fast @05:16:46.30 Air.seek :locked @05:16:47.88
Carousel.reconfigure :locked @05:16:48.51 Powering cartridge bus @05:16:51.18 Gate.setIR 1750,5000,0,6 @05:16:51.30 Gate.setIR 0,5000,0,6 @05:16:51.37 PuckHeater.configure :default @05:16:51.42 Gate.power :heat,:ON,limit to 5000mA @05:16:52.09 Mounted lysis Cartridge board#Q05065 @05:16:52.12 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:16:53.66 SPR.command "read_sequences" @05:16:54.38 SPR.command "stats_on" @05:16:54.54 SPR.command "start 0 1" @05:16:55.24 SPR.start /var/log/esp/spr23may0205h.spr.bz2 @05:16:57.23 Waiting for baseline SPR stabilization @05:16:57.25 Handoff.select :waste @05:17:03.96 Syringe tare is 18% of full scale @05:17:03.99 PinPort.in :locking @05:17:04.05 PinPort.seek :locked @05:17:04.53 Archive.in :fast @05:17:04.60 Lysis2.in :fast @05:17:04.70 Lysis1.seek 1000uL @05:17:04.80 Archive.seek :full @05:17:04.89 Lysis2.seek 1000uL @05:17:09.44 delayUntil "05:26:56.80" @05:26:56.81 Lysis2.in :DAmix @05:26:56.93 Lysis1.in :DAmix @05:26:57.50 Lysis2.seek 750uL @05:26:57.57 Lysis1.seek 750uL @05:27:32.67 delay 5 seconds @05:27:37.69 Handoff.select SPR @05:27:37.82 SPR.command "start 2 14" @05:27:38.51 SPR stabilized @05:27:38.66 delay 10 seconds @05:27:48.68 SPR.command "start 1 0" @05:27:51.88 Waiting up to 4:00 for SPR to enter state 1 @05:29:56.16 SPR.command "hold_on" @05:29:57.55 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:29:58.17 Lysis2.seek 450uL @05:29:58.22 Lysis1.seek 450uL @05:30:40.28 delay 15 seconds @05:30:55.30 SPR.command "hold_off" @05:30:58.20 Waiting up to 38:00 for SPR to enter state 7 @05:48:57.82 SPRsummary:11.4RIU,11RIU,7.77RIU,9.36RIU after 2030s @05:55:35.21 delay 10 seconds @05:55:45.23 SPR.command "start 1 0" @05:55:47.89 Waiting up to 4:00 for SPR to enter state 1 @05:57:52.18 SPR.command "hold_on" @05:57:53.57 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:57:54.15 Lysis2.seek 300uL @05:57:54.22 Lysis1.seek 300uL @05:58:15.31 delay 15 seconds @05:58:30.33 SPR.command "hold_off" @05:58:33.41 Waiting up to 38:00 for SPR to enter state 7 @06:23:10.39 delay 10 seconds @06:23:20.41 SPR.command "start 1 0" @06:23:24.05 Waiting up to 4:00 for SPR to enter state 1 @06:25:28.80 SPR.command "hold_on" @06:25:29.72 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:25:30.30 Lysis2.seek 150uL @06:25:30.36 Lysis1.seek 150uL @06:25:51.45 delay 15 seconds @06:26:06.47 SPR.command "hold_off" @06:26:09.57 Waiting up to 38:00 for SPR to enter state 7 @06:50:46.54 delay 10 seconds @06:50:56.56 SPR.command "start 1 0" @06:50:59.34 Waiting up to 4:00 for SPR to enter state 1 @06:53:03.54 SPR.command "hold_on" @06:53:05.45 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:05.99 Lysis2.seek 0uL @06:53:06.03 Lysis1.seek 0uL @06:53:27.21 delay 15 seconds @06:53:42.23 SPR.command "hold_off" @06:53:44.77 Waiting up to 38:00 for SPR to enter state 7 @07:18:20.66 SPR analysis completed @07:18:22.78 Ended log spr23may0205h.spr.bz2 @07:18:22.80 SPR.command "stats_off" @07:18:23.20 Handoff.select :close @07:18:23.35
Gate.power :heat,:OFF @07:18:23.36 Gate.power :cartridge,:OFF @07:18:23.44 PinPort.in :unlocking @07:18:23.95 PinPort.seek :clear @07:18:24.42 Carousel.in :default @07:18:24.54 Carousel.home.seek 29 @07:18:24.87 Plunge.seek :locked @07:18:25.04 Archive.in :unlocking @07:18:25.24 Archive.seek :clear @07:18:25.33 Air.in :unlocking @07:18:25.48 Air.seek :clear @07:18:25.62 Lysis2.in :unlocking @07:18:25.72 Lysis2.seek :clear @07:18:25.78 Lysis2.seek :clear @07:18:30.25 Slide::Error in MAIN -- Lysis Syringe #2 (LyS2) aborted at 1uL (sensed 7uL) @07:18:30.27
Retry #1 of 2 @07:18:30.43 Gate.power :motor,:OFF @07:18:30.44 Gate.power :core,:OFF @07:18:30.46 Gate.power :analytic1,:OFF @07:18:30.47 Gate.power :analytic2,:OFF @07:18:30.49 Gate.power :analytic3,:OFF @07:18:30.50 Gate.power :main,:OFF @07:18:34.27 Gate.power :main,:ON,limit to 5000mA @07:18:34.29 Gate.power :analytic1,:ON,limit to 5000mA @07:18:34.30 Gate.power :analytic2,:ON,limit to 5000mA @07:18:34.32 Gate.power :analytic3,:ON,limit to 5000mA @07:18:34.38 Powering core bus @07:18:36.74 SPR Module 1, FW Rev. B, FPGA Rev. C @07:18:37.16
Archive.configure :default @07:18:37.25 Air.configure :default @07:18:37.32 Lysis1.configure :default @07:18:37.39 Lysis2.configure :unlocking @07:18:37.47 Plunge.configure :default @07:18:37.56 SamplePump.configure :default @07:18:37.65 Toroid.configure :default @07:18:37.74 Carousel.configure :default @07:18:37.83 Pressure.configure :default @07:18:37.92 ExtraPump.configure :default @07:18:37.99 PinPort.configure :default @07:18:38.05 All elf controllers running firmware version 4.83 @07:18:38.07 Gate.power :motor,:ON,limit to 5000mA @07:18:38.17 Archive.setRawPosition! 28 @07:18:38.28 Air.setRawPosition! 22 @07:18:38.39 Lysis1.setRawPosition! -8325 @07:18:38.50 Lysis2.setRawPosition! 21 @07:18:38.61 Plunge.setRawPosition! -1235 @07:18:38.72 Toroid.setRawPosition! -468 @07:18:38.83 Carousel.setRawPosition! 6999 @07:18:38.94 PinPort.setRawPosition! -2 @07:18:39.26 Carousel.home.seek 29 @07:18:39.59 Plunge.home.seek :locked @07:18:40.28 Archive.in :unlocking @07:18:40.40 Archive.home.seek :clear @07:18:40.46 Air.in :unlocking @07:18:40.70 Air.home.seek :clear @07:18:40.79 Lysis1.in :unlocking @07:18:40.95 Lysis1.home.seek :clear @07:18:41.11 Lysis2.home.seek :clear @07:18:45.54
Gate.power :motor,:OFF @07:18:45.56 Gate.power :core,:OFF @07:18:45.57 Gate.power :analytic1,:OFF @07:18:45.58 Gate.power :analytic2,:OFF @07:18:45.60 Gate.power :analytic3,:OFF @07:18:45.61 Gate.power :main,:OFF @07:18:45.62 Safely stopped and ready to power off @07:18:49.38 Gate.power :main,:ON,limit to 5000mA @07:18:49.40 Gate.power :analytic1,:ON,limit to 5000mA @07:18:49.41 Gate.power :analytic2,:ON,limit to 5000mA @07:18:49.43 Gate.power :analytic3,:ON,limit to 5000mA @07:18:49.49 Powering core bus @07:18:51.86 SPR Module 1, FW Rev. B, FPGA Rev. C @07:18:52.25
Archive.configure :default @07:18:52.34 Air.configure :default @07:18:52.40 Lysis1.configure :default @07:18:52.47 Lysis2.configure :unlocking @07:18:52.55 Plunge.configure :default @07:18:52.64 SamplePump.configure :default @07:18:52.73 Toroid.configure :default @07:18:52.82 Carousel.configure :default @07:18:52.91 Pressure.configure :default @07:18:53.00 ExtraPump.configure :default @07:18:53.06 PinPort.configure :default @07:18:53.14 All elf controllers running firmware version 4.83 @07:18:53.17 Gate.power :motor,:ON,limit to 5000mA @07:18:53.44 Archive.home.jog 0 @07:18:54.15 PinPort.home.jog 0 @07:18:54.22 Carousel.home.jog 0 @07:18:54.24 Plunge.home.jog 0 @07:18:54.27 Lysis2.in :default @07:18:54.31 Air.home.jog 0 @07:18:54.34 Toroid.home.jog 0 @07:18:54.38 Lysis1.home.jog 0 @07:18:54.76 Lysis2.home.jog 0 @07:18:55.14 Archive.in :homing @07:18:55.46 Archive.jog 1395 @07:18:55.66 Toroid.in :homing @07:18:55.73 Plunge.in :homing @07:18:55.76 Lysis1.in :homing @07:18:55.81 Lysis2.in :homing @07:18:55.87 PinPort.in :homing @07:18:55.90 Air.in :homing @07:18:56.70 Plunge.jog 1541 @07:18:56.74 Toroid.jog 616 @07:18:56.76 Lysis1.jog 181 @07:18:56.78 PinPort.jog -109 @07:18:56.82 Lysis2.jog 150 @07:18:56.89 Air.jog 1391 @07:18:57.29 Archive.setRawPosition! 50 @07:18:57.38 PinPort.setRawPosition! 0 @07:18:57.40 Archive.seek :home @07:18:57.50 PinPort.seek :home @07:18:57.66 Lysis2.setRawPosition! 50 @07:18:57.69 Lysis1.setRawPosition! 50 @07:18:57.71 PinPort.in :default @07:18:57.85 Lysis2.seek :home @07:18:57.90 Lysis1.seek :home @07:18:58.04 Archive.in :default @07:18:58.18 Plunge.setRawPosition! 50 @07:18:58.27 Lysis2.in :unlocking @07:18:58.31 Lysis1.in :default @07:18:58.34 Plunge.seek :home @07:18:58.54 Air.setRawPosition! 50 @07:18:58.59 Air.seek :home @07:18:58.71 Plunge.in :default @07:18:58.87 Air.in :default @07:18:59.04 Toroid.setRawPosition! 0 @07:18:59.11 Toroid.seek :home @07:18:59.22 Toroid.in :default @07:18:59.30
Toroid.seek :clear @07:19:00.91 Carousel.in :default @07:19:01.04 Carousel.home.seek 29 @07:19:01.30 Selecting Cartridge 28 @07:19:01.59 Carousel.seek 28 @07:19:02.12 Lysis1.in :fast @07:19:02.18 Lysis1.seek :locked @07:19:04.81 Plunge.seek :locked @07:19:04.97 Air.in :fast @07:19:05.03 Air.seek :locked @07:19:06.60
Carousel.reconfigure :locked @07:19:06.76 Powering cartridge bus @07:19:09.40 Gate.setIR 1750,5000,0,6 @07:19:09.52 Gate.setIR 0,5000,0,6 @07:19:09.58 PuckHeater.configure :default @07:19:09.64 Gate.power :heat,:ON,limit to 5000mA @07:19:10.31 Mounted lysis Cartridge board#Q05062 @07:19:10.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @07:19:12.43 SPR.command "read_sequences" @07:19:12.53 SPR.command "stats_on" @07:19:12.69 SPR.command "start 0 1" @07:19:13.40 SPR.start /var/log/esp/spr23may0207h.spr.bz2 @07:19:15.90 Waiting for baseline SPR stabilization @07:19:15.92 Handoff.select :waste @07:19:22.61 Syringe tare is 18% of full scale @07:19:22.63 PinPort.in :locking @07:19:22.69 PinPort.seek :locked @07:19:23.19 Archive.in :fast @07:19:23.25 Lysis2.in :fast @07:19:23.31 Lysis1.seek 1000uL @07:19:23.51 Archive.seek :full @07:19:23.60 Lysis2.seek 1000uL @07:19:28.17 delayUntil "07:29:15.87" @07:29:15.89 Lysis2.in :DAmix @07:29:16.01 Lysis1.in :DAmix @07:29:16.53 Lysis2.seek 750uL @07:29:16.58 Lysis1.seek 750uL @07:29:51.72 delay 5 seconds @07:29:56.74 Handoff.select SPR @07:29:56.86 SPR.command "start 2 14" @07:29:57.60 SPR stabilized @07:29:57.76 delay 10 seconds @07:30:07.78 SPR.command "start 1 0" @07:30:10.97 Waiting up to 4:00 for SPR to enter state 1 @07:32:15.27 SPR.command "hold_on" @07:32:16.65 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @07:32:17.28 Lysis2.seek 450uL @07:32:17.34 Lysis1.seek 450uL @07:32:59.42 delay 15 seconds @07:33:14.44 SPR.command "hold_off" @07:33:17.30 Waiting up to 38:00 for SPR to enter state 7 @07:51:16.91 SPRsummary:-12.3RIU,27.4RIU,21.8RIU,36.1RIU after 2031s @07:57:54.27 delay 10 seconds @07:58:04.29 SPR.command "start 1 0" @07:58:06.99 Waiting up to 4:00 for SPR to enter state 1 @08:00:11.27 SPR.command "hold_on" @08:00:12.66 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:00:13.30 Lysis2.seek 300uL @08:00:13.36 Lysis1.seek 300uL @08:00:34.45 delay 15 seconds @08:00:49.47 SPR.command "hold_off" @08:00:52.51 Waiting up to 38:00 for SPR to enter state 7 @08:25:29.48 delay 10 seconds @08:25:39.51 SPR.command "start 1 0" @08:25:42.19 Waiting up to 4:00 for SPR to enter state 1 @08:27:46.49 SPR.command "hold_on" @08:27:47.87 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:27:48.45 Lysis2.seek 150uL @08:27:48.51 Lysis1.seek 150uL @08:28:09.59 delay 15 seconds @08:28:24.61 SPR.command "hold_off" @08:28:27.71 Waiting up to 38:00 for SPR to enter state 7 @08:53:04.69 delay 10 seconds @08:53:14.72 SPR.command "start 1 0" @08:53:17.40 Waiting up to 4:00 for SPR to enter state 1 @08:55:21.70 SPR.command "hold_on" @08:55:23.08 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:23.69 Lysis2.seek 0uL @08:55:23.75 Lysis1.seek 0uL @08:55:44.85 delay 15 seconds @08:55:59.87 SPR.command "hold_off" @08:56:02.92 Waiting up to 38:00 for SPR to enter state 7 @09:20:40.24 SPR analysis completed @09:20:42.34 Ended log spr23may0207h.spr.bz2 @09:20:42.36 SPR.command "stats_off" @09:20:43.50 Handoff.select :close @09:20:43.65
Gate.power :heat,:OFF @09:20:43.66 Gate.power :cartridge,:OFF @09:20:43.75 PinPort.in :unlocking @09:20:43.81 PinPort.seek :clear @09:20:44.27 Carousel.in :default @09:20:44.40 Carousel.home.seek 28 @09:20:44.73 Plunge.seek :locked @09:20:44.89 Archive.in :unlocking @09:20:45.05 Archive.seek :clear @09:20:45.17 Air.in :unlocking @09:20:45.33 Air.seek :clear @09:20:45.41 Lysis1.in :unlocking @09:20:45.58 Lysis1.seek :clear @09:20:45.69 Lysis2.in :unlocking @09:20:45.76 Lysis2.seek :clear @09:20:50.35
Gate.power :motor,:OFF @09:20:50.36 Gate.power :core,:OFF @09:20:50.38 Gate.power :analytic1,:OFF @09:20:50.39 Gate.power :analytic2,:OFF @09:20:50.40 Gate.power :analytic3,:OFF @09:20:50.42 Gate.power :main,:OFF @09:20:50.43 Safely stopped and ready to power off @09:20:54.19 Gate.power :main,:ON,limit to 5000mA @09:20:54.20 Gate.power :analytic1,:ON,limit to 5000mA @09:20:54.22 Gate.power :analytic2,:ON,limit to 5000mA @09:20:54.23 Gate.power :analytic3,:ON,limit to 5000mA @09:20:54.30 Powering core bus @09:20:56.66 SPR Module 1, FW Rev. B, FPGA Rev. C @09:20:57.08
Archive.configure :default @09:20:57.20 Air.configure :default @09:20:57.26 Lysis1.configure :default @09:20:57.32 Lysis2.configure :unlocking @09:20:57.42 Plunge.configure :default @09:20:57.50 SamplePump.configure :default @09:20:57.60 Toroid.configure :default @09:20:57.69 Carousel.configure :default @09:20:57.77 Pressure.configure :default @09:20:57.86 ExtraPump.configure :default @09:20:57.92 PinPort.configure :default @09:20:57.99 All elf controllers running firmware version 4.83 @09:20:58.01 Gate.power :motor,:ON,limit to 5000mA @09:20:58.28 Archive.home.jog 0 @09:20:58.90 Lysis2.in :default @09:20:59.02 Carousel.home.jog 0 @09:20:59.07 Toroid.home.jog 0 @09:20:59.10 Air.home.jog 0 @09:20:59.15 PinPort.home.jog 0 @09:20:59.73 Lysis1.home.jog 0 @09:20:59.76 Plunge.home.jog 0 @09:21:00.09 Lysis2.home.jog 0 @09:21:00.48 Archive.in :homing @09:21:00.81 Archive.jog 1373 @09:21:01.01 Toroid.in :homing @09:21:01.09 Plunge.in :homing @09:21:01.14 Lysis1.in :homing @09:21:01.19 Lysis2.in :homing @09:21:01.24 PinPort.in :homing @09:21:01.28 Air.in :homing @09:21:01.55 Toroid.jog 617 @09:21:01.60 Plunge.jog 1525 @09:21:01.63 Lysis1.jog 1394 @09:21:01.66 Lysis2.jog 1397 @09:21:01.70 PinPort.jog -110 @09:21:01.76 Air.jog 1371 @09:21:02.12 Archive.setRawPosition! 50 @09:21:02.21 PinPort.setRawPosition! 0 @09:21:02.24 Archive.seek :home @09:21:02.34 PinPort.seek :home @09:21:02.45 PinPort.in :default @09:21:02.57 Archive.in :default @09:21:03.03 Plunge.setRawPosition! 50 @09:21:03.15 Lysis1.setRawPosition! 50 @09:21:03.19 Plunge.seek :home @09:21:03.22 Lysis2.setRawPosition! 50 @09:21:03.38 Lysis2.seek :home @09:21:03.41 Lysis1.seek :home @09:21:03.59 Plunge.in :default @09:21:03.62 Air.setRawPosition! 50 @09:21:03.77 Lysis2.in :unlocking @09:21:03.81 Air.seek :home @09:21:03.95 Lysis1.in :default @09:21:03.98 Toroid.setRawPosition! 0 @09:21:04.07 Toroid.seek :home @09:21:04.21 Air.in :default @09:21:04.24 Toroid.in :default @09:21:04.62
Toroid.seek :clear @09:21:06.23 Carousel.in :default @09:21:06.36 Carousel.home.seek 28 @09:21:06.62 Selecting Cartridge 27 @09:21:06.94 Carousel.seek 27 @09:21:07.45 Lysis1.in :fast @09:21:07.51 Lysis1.seek :locked @09:21:10.13 Plunge.seek :locked @09:21:10.79 Air.in :fast @09:21:10.85 Air.seek :locked @09:21:12.43
Carousel.reconfigure :locked @09:21:12.58 Powering cartridge bus @09:21:15.24 Gate.setIR 1750,5000,0,6 @09:21:15.37 Gate.setIR 0,5000,0,6 @09:21:15.43 PuckHeater.configure :default @09:21:15.49 Gate.power :heat,:ON,limit to 5000mA @09:21:16.16 Mounted lysis Cartridge board#Q05058 @09:21:16.19 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:21:17.72 SPR.command "read_sequences" @09:21:18.28 SPR.command "stats_on" @09:21:18.44 SPR.command "start 0 1" @09:21:19.14 SPR.start /var/log/esp/spr23may0209h.spr.bz2 @09:21:21.16 Waiting for baseline SPR stabilization @09:21:21.18 Handoff.select :waste @09:21:27.91 Syringe tare is 18% of full scale @09:21:27.94 PinPort.in :locking @09:21:28.00 PinPort.seek :locked @09:21:28.48 Archive.in :fast @09:21:28.57 Lysis2.in :fast @09:21:28.65 Lysis1.seek 1000uL @09:21:28.76 Archive.seek :full @09:21:28.83 Lysis2.seek 1000uL @09:21:33.38 delayUntil "09:31:20.70" @09:27:22.06 Session Begun @09:27:27.72 -> showlog -50 @09:29:09.50 -> showlog @09:31:20.71 Lysis2.in :DAmix @09:31:20.86 Lysis1.in :DAmix @09:31:21.49 Lysis2.seek 750uL @09:31:21.54 Lysis1.seek 750uL @09:31:56.71 delay 5 seconds @09:32:01.74 Handoff.select SPR @09:32:01.89 SPR.command "start 2 14" @09:32:02.40 SPR stabilized @09:32:02.59 delay 10 seconds @09:32:12.61 SPR.command "start 1 0" @09:32:15.77 Waiting up to 4:00 for SPR to enter state 1 @09:34:20.06 SPR.command "hold_on" @09:34:21.45 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @09:34:22.11 Lysis2.seek 450uL @09:34:22.18 Lysis1.seek 450uL @09:35:04.27 delay 15 seconds @09:35:19.29 SPR.command "hold_off" @09:35:22.10 Waiting up to 38:00 for SPR to enter state 7 @09:53:21.72 SPRsummary:5.06RIU,20.3RIU,19.9RIU,25.1RIU after 2030s @09:59:59.08 delay 10 seconds @10:00:09.10 SPR.command "start 1 0" @10:00:11.79 Waiting up to 4:00 for SPR to enter state 1 @10:02:16.08 SPR.command "hold_on" @10:02:17.47 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:02:18.14 Lysis2.seek 300uL @10:02:18.22 Lysis1.seek 300uL @10:02:39.33 delay 15 seconds @10:02:54.36 SPR.command "hold_off" @10:02:57.31 Waiting up to 38:00 for SPR to enter state 7 @10:27:34.29 delay 10 seconds @10:27:44.31 SPR.command "start 1 0" @10:27:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:29:51.29 SPR.command "hold_on" @10:29:53.20 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:29:53.80 Lysis2.seek 150uL @10:29:53.84 Lysis1.seek 150uL @10:30:14.99 delay 15 seconds @10:30:30.02 SPR.command "hold_off" @10:30:33.47 Waiting up to 38:00 for SPR to enter state 7 @10:51:52.17 Session Ended @10:55:09.50 delay 10 seconds @10:55:19.52 SPR.command "start 1 0" @10:55:22.21 Waiting up to 4:00 for SPR to enter state 1 @10:57:26.50 SPR.command "hold_on" @10:57:28.40 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:28.98 Lysis2.seek 0uL @10:57:29.02 Lysis1.seek 0uL @10:57:50.15 delay 15 seconds @10:58:05.19 SPR.command "hold_off" @10:58:07.73 Waiting up to 38:00 for SPR to enter state 7 @11:22:43.62 SPR analysis completed @11:22:45.72 Ended log spr23may0209h.spr.bz2 @11:22:45.74 SPR.command "stats_off" @11:22:46.12 Handoff.select :close @11:22:46.65
Gate.power :heat,:OFF @11:22:46.67 Gate.power :cartridge,:OFF @11:22:46.76 PinPort.in :unlocking @11:22:46.82 PinPort.seek :clear @11:22:47.28 Carousel.in :default @11:22:47.41 Carousel.home.seek 27 @11:22:47.74 Plunge.seek :locked @11:22:47.91 Archive.in :unlocking @11:22:48.06 Archive.seek :clear @11:22:48.12 Air.in :unlocking @11:22:48.32 Air.seek :clear @11:22:48.41 Lysis1.in :unlocking @11:22:48.58 Lysis1.seek :clear @11:22:48.62 Lysis2.in :unlocking @11:22:48.74 Lysis2.seek :clear @11:22:53.32
Gate.power :motor,:OFF @11:22:53.34 Gate.power :core,:OFF @11:22:53.35 Gate.power :analytic1,:OFF @11:22:53.37 Gate.power :analytic2,:OFF @11:22:53.38 Gate.power :analytic3,:OFF @11:22:53.39 Gate.power :main,:OFF @11:22:53.41 Safely stopped and ready to power off @11:22:57.16 Gate.power :main,:ON,limit to 5000mA @11:22:57.18 Gate.power :analytic1,:ON,limit to 5000mA @11:22:57.20 Gate.power :analytic2,:ON,limit to 5000mA @11:22:57.21 Gate.power :analytic3,:ON,limit to 5000mA @11:22:57.28 Powering core bus @11:22:59.64 SPR Module 1, FW Rev. B, FPGA Rev. C @11:23:00.03
Archive.configure :default @11:23:00.12 Air.configure :default @11:23:00.19 Lysis1.configure :default @11:23:00.25 Lysis2.configure :unlocking @11:23:00.34 Plunge.configure :default @11:23:00.43 SamplePump.configure :default @11:23:00.53 Toroid.configure :default @11:23:00.61 Carousel.configure :default @11:23:00.70 Pressure.configure :default @11:23:00.79 ExtraPump.configure :default @11:23:00.85 PinPort.configure :default @11:23:00.92 All elf controllers running firmware version 4.83 @11:23:00.94 Gate.power :motor,:ON,limit to 5000mA @11:23:01.16 Archive.home.jog 0 @11:23:01.93 Carousel.home.jog 0 @11:23:01.99 Toroid.home.jog 0 @11:23:02.02 Lysis2.in :default @11:23:02.05 Lysis1.home.jog 0 @11:23:02.10 PinPort.home.jog 0 @11:23:02.14 Air.home.jog 0 @11:23:02.16 Plunge.home.jog 0 @11:23:02.43 Lysis2.home.jog 0 @11:23:03.58 Archive.in :homing @11:23:03.88 Archive.jog 1373 @11:23:03.96 Toroid.in :homing @11:23:03.99 Lysis2.in :homing @11:23:04.03 Air.in :homing @11:23:04.07 Plunge.in :homing @11:23:04.12 Lysis1.in :homing @11:23:04.15 PinPort.in :homing @11:23:04.45 Lysis2.jog 1392 @11:23:04.50 Toroid.jog 620 @11:23:04.53 Air.jog 1373 @11:23:04.56 Plunge.jog 1524 @11:23:04.59 PinPort.jog -109 @11:23:04.63 Lysis1.jog 1394 @11:23:05.06 PinPort.setRawPosition! 0 @11:23:05.12 PinPort.seek :home @11:23:05.23 PinPort.in :default @11:23:05.36 Archive.setRawPosition! 50 @11:23:05.41 Archive.seek :home @11:23:05.69 Archive.in :default @11:23:05.82 Lysis2.setRawPosition! 50 @11:23:05.87 Lysis2.seek :home @11:23:05.97 Air.setRawPosition! 50 @11:23:06.05 Lysis1.setRawPosition! 50 @11:23:06.09 Air.seek :home @11:23:06.18 Lysis1.seek :home @11:23:06.32 Lysis2.in :unlocking @11:23:06.35 Plunge.setRawPosition! 50 @11:23:06.48 Plunge.seek :home @11:23:06.58 Air.in :default @11:23:06.61 Lysis1.in :default @11:23:06.77 Plunge.in :default @11:23:06.87 Toroid.setRawPosition! 0 @11:23:06.92 Toroid.seek :home @11:23:07.01 Toroid.in :default @11:23:07.12
Toroid.seek :clear @11:23:08.76 Carousel.in :default @11:23:08.88 Carousel.home.seek 27 @11:23:09.17 Selecting Cartridge 26 @11:23:09.48 Carousel.seek 26 @11:23:10.00 Lysis1.in :fast @11:23:10.06 Lysis1.seek :locked @11:23:12.68 Plunge.seek :locked @11:23:12.84 Air.in :fast @11:23:12.90 Air.seek :locked @11:23:14.47
Carousel.reconfigure :locked @11:23:15.16 Powering cartridge bus @11:23:17.80 Gate.setIR 1750,5000,0,6 @11:23:17.92 Gate.setIR 0,5000,0,6 @11:23:17.99 PuckHeater.configure :default @11:23:18.05 Gate.power :heat,:ON,limit to 5000mA @11:23:18.72 Mounted lysis Cartridge board#Q05054 @11:23:18.75 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:23:20.33 SPR.command "read_sequences" @11:23:20.40 SPR.command "stats_on" @11:23:20.55 SPR.command "start 0 1" @11:23:21.26 SPR.start /var/log/esp/spr23may0211h.spr.bz2 @11:23:23.17 Waiting for baseline SPR stabilization @11:23:23.18 Handoff.select :waste @11:23:29.88 Syringe tare is 18% of full scale @11:23:29.91 PinPort.in :locking @11:23:29.97 PinPort.seek :locked @11:23:30.46 Archive.in :fast @11:23:30.53 Lysis2.in :fast @11:23:30.60 Lysis1.seek 1000uL @11:23:30.75 Lysis2.seek 1000uL @11:23:30.80 Archive.seek :full @11:23:35.34 delayUntil "11:33:22.73" @11:33:22.74 Lysis2.in :DAmix @11:33:22.87 Lysis1.in :DAmix @11:33:23.44 Lysis2.seek 750uL @11:33:23.53 Lysis1.seek 750uL @11:33:58.62 delay 5 seconds @11:34:03.64 Handoff.select SPR @11:34:03.77 SPR.command "start 2 14" @11:34:04.44 SPR stabilized @11:34:04.59 delay 10 seconds @11:34:14.61 SPR.command "start 1 0" @11:34:18.33 Waiting up to 4:00 for SPR to enter state 1 @11:36:23.05 SPR.command "hold_on" @11:36:24.43 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @11:36:25.04 Lysis2.seek 450uL @11:36:25.10 Lysis1.seek 450uL @11:37:07.20 delay 15 seconds @11:37:22.23 SPR.command "hold_off" @11:37:25.08 Waiting up to 38:00 for SPR to enter state 7 @11:38:38.03 Session Begun @11:38:42.48 -> cd /var/log/esp @11:38:42.50 SyntaxError in bill -- compile error bill:1: unknown regexp options - lg @11:38:51.82 -> cd /var/log/esp @11:38:51.84 SyntaxError in bill -- compile error bill:2: unknown regexp options - lg @11:39:06.58 -> exit @11:39:06.60 Session Ended @11:40:32.08 Session Begun @11:40:38.96 -> showlog @11:40:57.86 -> exit @11:40:57.88 Session Ended @11:55:24.69 SPRsummary:4.9RIU,17.5RIU,18.1RIU,20.9RIU after 2031s @11:58:21.84 Session Begun @11:58:25.97 -> showlog @12:02:02.06 delay 10 seconds @12:02:12.09 SPR.command "start 1 0" @12:02:15.29 Waiting up to 4:00 for SPR to enter state 1 @12:04:20.01 SPR.command "hold_on" @12:04:21.39 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @12:04:22.06 Lysis2.seek 300uL @12:04:22.11 Lysis1.seek 300uL @12:04:43.27 delay 15 seconds @12:04:58.29 SPR.command "hold_off" @12:05:01.24 Waiting up to 38:00 for SPR to enter state 7 @12:29:38.68 delay 10 seconds @12:29:48.70 SPR.command "start 1 0" @12:29:51.87 Waiting up to 4:00 for SPR to enter state 1 @12:31:56.16 SPR.command "hold_on" @12:31:57.55 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @12:31:58.15 Lysis2.seek 150uL @12:31:58.28 Lysis1.seek 150uL @12:32:19.34 delay 15 seconds @12:32:34.37 SPR.command "hold_off" @12:32:37.39 Waiting up to 38:00 for SPR to enter state 7 @12:57:14.85 delay 10 seconds @12:57:24.87 SPR.command "start 1 0" @12:57:28.03 Waiting up to 4:00 for SPR to enter state 1 @12:59:32.31 SPR.command "hold_on" @12:59:33.70 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @12:59:34.32 Lysis2.seek 0uL @12:59:34.39 Lysis1.seek 0uL @12:59:55.53 delay 15 seconds @13:00:10.55 SPR.command "hold_off" @13:00:13.54 Waiting up to 38:00 for SPR to enter state 7 @13:02:41.15 Session Ended @13:24:50.38 SPR analysis completed @13:24:52.69 Ended log spr23may0211h.spr.bz2 @13:24:52.70 SPR.command "stats_off" @13:24:53.39 Handoff.select :close @13:24:53.53
Gate.power :heat,:OFF @13:24:53.55 Gate.power :cartridge,:OFF @13:24:53.63 PinPort.in :unlocking @13:24:53.69 PinPort.seek :clear @13:24:54.16 Carousel.in :default @13:24:54.29 Carousel.home.seek 26 @13:24:54.62 Plunge.seek :locked @13:24:54.79 Archive.in :unlocking @13:24:54.94 Archive.seek :clear @13:24:55.07 Air.in :unlocking @13:24:55.21 Air.seek :clear @13:24:55.30 Lysis1.in :unlocking @13:24:55.45 Lysis1.seek :clear @13:24:55.51 Lysis2.in :unlocking @13:24:55.64 Lysis2.seek :clear @13:25:00.23
Gate.power :motor,:OFF @13:25:00.24 Gate.power :core,:OFF @13:25:00.25 Gate.power :analytic1,:OFF @13:25:00.27 Gate.power :analytic2,:OFF @13:25:00.28 Gate.power :analytic3,:OFF @13:25:00.29 Gate.power :main,:OFF @13:25:00.31 Safely stopped and ready to power off @13:25:04.06 Gate.power :main,:ON,limit to 5000mA @13:25:04.08 Gate.power :analytic1,:ON,limit to 5000mA @13:25:04.10 Gate.power :analytic2,:ON,limit to 5000mA @13:25:04.11 Gate.power :analytic3,:ON,limit to 5000mA @13:25:04.18 Powering core bus @13:25:06.54 SPR Module 1, FW Rev. B, FPGA Rev. C @13:25:06.93
Archive.configure :default @13:25:07.02 Air.configure :default @13:25:07.08 Lysis1.configure :default @13:25:07.17 Lysis2.configure :unlocking @13:25:07.26 Plunge.configure :default @13:25:07.35 SamplePump.configure :default @13:25:07.44 Toroid.configure :default @13:25:07.53 Carousel.configure :default @13:25:07.62 Pressure.configure :default @13:25:07.71 ExtraPump.configure :default @13:25:07.77 PinPort.configure :default @13:25:07.83 All elf controllers running firmware version 4.83 @13:25:07.85 Gate.power :motor,:ON,limit to 5000mA @13:25:08.31 Archive.home.jog 0 @13:25:08.76 Lysis1.home.jog 0 @13:25:08.86 Carousel.home.jog 0 @13:25:08.90 Toroid.home.jog 0 @13:25:09.51 Lysis2.in :default @13:25:09.58 PinPort.home.jog 0 @13:25:09.62 Air.home.jog 0 @13:25:09.67 Plunge.home.jog 0 @13:25:09.93 Lysis2.home.jog 0 @13:25:10.40 Archive.in :homing @13:25:10.73 Archive.jog 1374 @13:25:10.80 PinPort.in :homing @13:25:10.83 Plunge.in :homing @13:25:10.87 Lysis1.in :homing @13:25:10.93 Toroid.in :homing @13:25:10.98 Air.in :homing @13:25:11.03 Lysis2.in :homing @13:25:11.30 Plunge.jog 1524 @13:25:11.33 PinPort.jog -108 @13:25:11.47 Toroid.jog 617 @13:25:11.52 Lysis2.jog 1393 @13:25:11.55 Air.jog 1370 @13:25:11.60 Lysis1.jog 1393 @13:25:11.91 PinPort.setRawPosition! 0 @13:25:11.96 PinPort.seek :home @13:25:12.06 PinPort.in :default @13:25:12.15 Archive.setRawPosition! 50 @13:25:12.21 Archive.seek :home @13:25:12.48 Archive.in :default @13:25:12.67 Plunge.setRawPosition! 50 @13:25:12.72 Plunge.seek :home @13:25:12.84 Lysis2.setRawPosition! 50 @13:25:12.89 Lysis2.seek :home @13:25:12.98 Air.setRawPosition! 50 @13:25:13.06 Air.seek :home @13:25:13.17 Lysis1.setRawPosition! 50 @13:25:13.24 Plunge.in :default @13:25:13.30 Lysis1.seek :home @13:25:13.45 Air.in :default @13:25:13.48 Lysis2.in :unlocking @13:25:13.63 Lysis1.in :default @13:25:13.81 Toroid.setRawPosition! 0 @13:25:13.87 Toroid.seek :home @13:25:13.94 Toroid.in :default @13:25:14.04
Toroid.seek :clear @13:25:15.65 Carousel.in :default @13:25:15.77 Carousel.home.seek 26 @13:25:16.02 Selecting Cartridge 25 @13:25:16.33 Carousel.seek 25 @13:25:16.85 Lysis1.in :fast @13:25:16.92 Lysis1.seek :locked @13:25:19.53 Plunge.seek :locked @13:25:20.19 Air.in :fast @13:25:20.26 Air.seek :locked @13:25:21.84
Carousel.reconfigure :locked @13:25:22.00 Powering cartridge bus @13:25:24.64 Gate.setIR 1750,5000,0,6 @13:25:24.76 Gate.setIR 0,5000,0,6 @13:25:24.83 PuckHeater.configure :default @13:25:24.89 Gate.power :heat,:ON,limit to 5000mA @13:25:25.57 Mounted lysis Cartridge board#Q05048 @13:25:25.61 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:25:27.16 SPR.command "read_sequences" @13:25:27.22 SPR.command "stats_on" @13:25:27.39 SPR.command "start 0 1" @13:25:28.09 SPR.start /var/log/esp/spr23may0213h.spr.bz2 @13:25:30.07 Waiting for baseline SPR stabilization @13:25:30.08 Handoff.select :waste @13:25:36.78 Syringe tare is 18% of full scale @13:25:36.81 PinPort.in :locking @13:25:36.87 PinPort.seek :locked @13:25:37.35 Archive.in :fast @13:25:37.43 Lysis2.in :fast @13:25:37.50 Lysis1.seek 1000uL @13:25:37.69 Lysis2.seek 1000uL @13:25:37.73 Archive.seek :full @13:25:42.26 delayUntil "13:35:29.63" @13:35:29.64 Lysis2.in :DAmix @13:35:29.77 Lysis1.in :DAmix @13:35:30.34 Lysis2.seek 750uL @13:35:30.39 Lysis1.seek 750uL @13:36:05.57 delay 5 seconds @13:36:10.59 Handoff.select SPR @13:36:10.72 SPR.command "start 2 14" @13:36:11.34 SPR stabilized @13:36:11.49 delay 10 seconds @13:36:21.51 SPR.command "start 1 0" @13:36:24.71 Waiting up to 4:00 for SPR to enter state 1 @13:38:28.99 SPR.command "hold_on" @13:38:30.38 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @13:38:31.03 Lysis2.seek 450uL @13:38:31.07 Lysis1.seek 450uL @13:39:13.18 delay 15 seconds @13:39:28.20 SPR.command "hold_off" @13:39:31.04 Waiting up to 38:00 for SPR to enter state 7 @13:42:40.90 Session Begun @13:42:42.66 -> showlog @13:42:49.49 -> showlog -50 @13:50:14.28 -> showlog -50 @13:57:30.65 SPRsummary:4.45RIU,27.1RIU,26.5RIU,34.5RIU after 2030s @14:04:08.01 delay 10 seconds @14:04:18.03 SPR.command "start 1 0" @14:04:20.72 Waiting up to 4:00 for SPR to enter state 1 @14:06:25.02 SPR.command "hold_on" @14:06:26.40 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:06:27.05 Lysis2.seek 300uL @14:06:27.18 Lysis1.seek 300uL @14:06:48.28 delay 15 seconds @14:07:03.31 SPR.command "hold_off" @14:07:06.24 Waiting up to 38:00 for SPR to enter state 7 @14:26:56.12 Session Ended @14:31:43.25 delay 10 seconds @14:31:53.27 SPR.command "start 1 0" @14:31:55.93 Waiting up to 4:00 for SPR to enter state 1 @14:34:00.23 SPR.command "hold_on" @14:34:01.61 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:34:02.22 Lysis2.seek 150uL @14:34:02.26 Lysis1.seek 150uL @14:34:23.36 delay 15 seconds @14:34:38.38 SPR.command "hold_off" @14:34:41.97 Waiting up to 38:00 for SPR to enter state 7 @14:59:19.38 delay 10 seconds @14:59:29.40 SPR.command "start 1 0" @14:59:32.09 Waiting up to 4:00 for SPR to enter state 1 @15:01:36.37 SPR.command "hold_on" @15:01:37.76 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:01:38.37 Lysis2.seek 0uL @15:01:38.43 Lysis1.seek 0uL @15:01:59.53 delay 15 seconds @15:02:14.55 SPR.command "hold_off" @15:02:17.61 Waiting up to 38:00 for SPR to enter state 7 @15:02:52.69 Session Begun @15:02:57.36 -> showlog -50 @15:03:04.50 -> showlog @15:26:54.44 SPR analysis completed @15:26:56.56 Ended log spr23may0213h.spr.bz2 @15:26:56.58 SPR.command "stats_off" @15:26:57.45 Handoff.select :close @15:26:58.08
Gate.power :heat,:OFF @15:26:58.09 Gate.power :cartridge,:OFF @15:26:58.21 PinPort.in :unlocking @15:26:58.30 PinPort.seek :clear @15:26:58.79 Carousel.in :default @15:26:58.94 Carousel.home.seek 25 @15:26:59.33 Plunge.seek :locked @15:26:59.53 Archive.in :unlocking @15:26:59.70 Archive.seek :clear @15:26:59.76 Air.in :unlocking @15:26:59.99 Air.seek :clear @15:27:00.12 Lysis1.in :unlocking @15:27:00.30 Lysis1.seek :clear @15:27:00.40 Lysis2.in :unlocking @15:27:00.50 Lysis2.seek :clear @15:27:05.12
Gate.power :motor,:OFF @15:27:05.16 Gate.power :core,:OFF @15:27:05.19 Gate.power :analytic1,:OFF @15:27:05.23 Gate.power :analytic2,:OFF @15:27:05.26 Gate.power :analytic3,:OFF @15:27:05.29 Gate.power :main,:OFF @15:27:05.32 Safely stopped and ready to power off @15:27:05.80 Cartridge::None in MAIN -- Not enough standard cartridges remain @15:27:05.88 Session Ended @16:03:14.77PDT02-May-23
>BREAK< ESP3 Gateway v1.42 for core bus @16:03:14.80 >BREAK< ESP3 Gateway v1.42 for cartridge bus @16:03:17.99 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @16:03:18.58 Sea pressure (Abs) tare is 6% of full scale @16:03:26.51 Polling Can every 2:00 @16:03:26.55 Loading /home/esp/esp2/bin/processAll mission @16:03:26.77 Sea pressure (Abs) tare is 6% of full scale @16:03:26.78 Sampling default goal volumes @16:03:26.80 Options: {extraDilutions: [1,1,1]} Cartridge group: standard Spares: {spare: 0} @16:03:26.86 Gate.power :main,:ON,limit to 5000mA @16:03:26.87 Gate.power :analytic1,:ON,limit to 5000mA @16:03:26.89 Gate.power :analytic2,:ON,limit to 5000mA @16:03:26.90 Gate.power :analytic3,:ON,limit to 5000mA @16:03:27.06 Powering core bus @16:03:29.33 SPR Module 1, FW Rev. B, FPGA Rev. C @16:03:29.81
Archive.configure :default @16:03:29.90 Air.configure :default @16:03:29.96 Lysis1.configure :default @16:03:30.03 Lysis2.configure :default @16:03:30.11 Plunge.configure :default @16:03:30.20 SamplePump.configure :default @16:03:30.29 Toroid.configure :default @16:03:30.37 Carousel.configure :default @16:03:30.46 Pressure.configure :default @16:03:30.55 ExtraPump.configure :default @16:03:30.61 PinPort.configure :default @16:03:30.67 All elf controllers running firmware version 4.83 @16:03:30.69 Gate.power :motor,:ON,limit to 5000mA @16:03:30.92 Archive.home.jog 0 @16:03:31.97 PinPort.home.jog 0 @16:03:32.13 Carousel.home.jog 0 @16:03:32.15 Plunge.home.jog 0 @16:03:32.18 Air.home.jog 0 @16:03:32.20 Toroid.home.jog 0 @16:03:32.22 Lysis1.home.jog 0 @16:03:32.31 Lysis2.home.jog 0 @16:03:33.07 Archive.in :homing @16:03:33.19 Archive.jog 1377 @16:03:33.42 Toroid.in :homing @16:03:33.46 Plunge.in :homing @16:03:33.49 Lysis1.in :homing @16:03:33.53 Air.in :homing @16:03:33.58 PinPort.in :homing @16:03:33.63 Lysis2.in :homing @16:03:33.84 Plunge.jog 1520 @16:03:33.97 PinPort.jog -107 @16:03:34.03 Toroid.jog 615 @16:03:34.06 Lysis2.jog 1398 @16:03:34.09 Air.jog 1372 @16:03:34.12 Lysis1.jog 1395 @16:03:34.47 PinPort.setRawPosition! 0 @16:03:34.49 Archive.setRawPosition! 50 @16:03:34.57 PinPort.seek :home @16:03:34.66 Archive.seek :home @16:03:34.78 PinPort.in :default @16:03:34.97 Archive.in :default @16:03:35.15 Plunge.setRawPosition! 50 @16:03:35.21 Plunge.seek :home @16:03:35.43 Lysis2.setRawPosition! 50 @16:03:35.51 Lysis2.seek :home @16:03:35.61 Lysis1.setRawPosition! 50 @16:03:35.64 Air.setRawPosition! 50 @16:03:35.66 Plunge.in :default @16:03:35.79 Lysis1.seek :home @16:03:35.83 Air.seek :home @16:03:36.01 Lysis2.in :default @16:03:36.15 Lysis1.in :default @16:03:36.23 Air.in :default @16:03:36.34 Toroid.setRawPosition! 0 @16:03:36.38 Toroid.seek :home @16:03:36.48 Toroid.in :default @16:03:36.60
Toroid.seek :clear @16:03:38.21 Carousel.in :default @16:03:38.34 Carousel.home.seek 25 @16:03:38.61 Selecting Cartridge 24 @16:03:38.92 Carousel.seek 24 @16:03:39.44 Lysis1.in :fast @16:03:39.51 Lysis1.seek :locked @16:03:42.13 Plunge.seek :locked @16:03:42.30 Air.in :fast @16:03:42.36 Air.seek :locked @16:03:43.93
Carousel.reconfigure :locked @16:03:44.08 Powering cartridge bus @16:03:46.65 Gate.setIR 1750,5000,0,6 @16:03:46.77 Gate.setIR 0,5000,0,6 @16:03:46.84 PuckHeater.configure :default @16:03:46.90 Gate.power :heat,:ON,limit to 5000mA @16:03:47.64 Mounted lysis Cartridge board#Q05046 @16:03:47.68 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:03:49.29 SPR.command "read_sequences" @16:03:50.00 SPR.command "stats_on" @16:03:50.18 SPR.command "start 0 1" @16:03:50.86 SPR.start /var/log/esp/spr23may0216h.spr.bz2 @16:03:52.85 Waiting for baseline SPR stabilization @16:03:52.87 Handoff.select :waste @16:03:59.57 Syringe tare is 18% of full scale @16:03:59.59 PinPort.in :locking @16:03:59.66 PinPort.seek :locked @16:04:00.15 Archive.in :fast @16:04:00.22 Lysis2.in :fast @16:04:00.29 Lysis1.seek 1000uL @16:04:00.46 Archive.seek :full @16:04:00.49 Lysis2.seek 1000uL @16:04:05.05 delayUntil "16:13:52.39" @16:13:52.40 Lysis2.in :DAmix @16:13:52.53 Lysis1.in :DAmix @16:13:53.07 Lysis2.seek 750uL @16:13:53.10 Lysis1.seek 750uL @16:14:28.25 delay 5 seconds @16:14:33.27 Handoff.select SPR @16:14:33.40 SPR.command "start 2 14" @16:14:34.13 SPR stabilized @16:14:34.28 delay 10 seconds @16:14:44.30 SPR.command "start 1 0" @16:14:47.49 Waiting up to 4:00 for SPR to enter state 1 @16:16:51.75 SPR.command "hold_on" @16:16:53.18 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @16:16:53.76 Lysis2.seek 450uL @16:16:53.83 Lysis1.seek 450uL @16:17:35.87 delay 15 seconds @16:17:50.89 SPR.command "hold_off" @16:17:54.32 Waiting up to 38:00 for SPR to enter state 7 @16:35:54.37 SPRsummary:1.68RIU,214RIU,176RIU,295RIU after 2031s @16:42:31.74 delay 10 seconds @16:42:41.77 SPR.command "start 1 0" @16:42:44.46 Waiting up to 4:00 for SPR to enter state 1 @16:44:48.74 SPR.command "hold_on" @16:44:50.13 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:44:50.74 Lysis1.seek 300uL @16:44:50.77 Lysis2.seek 300uL @16:45:11.88 delay 15 seconds @16:45:26.90 SPR.command "hold_off" @16:45:29.98 Waiting up to 38:00 for SPR to enter state 7 @17:10:07.43 delay 10 seconds @17:10:17.45 SPR.command "start 1 0" @17:10:20.61 Waiting up to 4:00 for SPR to enter state 1 @17:12:24.89 SPR.command "hold_on" @17:12:26.29 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:12:26.85 Lysis2.seek 150uL @17:12:26.90 Lysis1.seek 150uL @17:12:48.02 delay 15 seconds @17:13:03.04 SPR.command "hold_off" @17:13:06.65 Waiting up to 38:00 for SPR to enter state 7 @17:37:44.05 delay 10 seconds @17:37:54.07 SPR.command "start 1 0" @17:37:56.76 Waiting up to 4:00 for SPR to enter state 1 @17:40:01.06 SPR.command "hold_on" @17:40:02.44 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:40:03.00 Lysis2.seek 0uL @17:40:03.05 Lysis1.seek 0uL @17:40:24.21 delay 15 seconds @17:40:39.23 SPR.command "hold_off" @17:40:42.28 Waiting up to 38:00 for SPR to enter state 7 @18:05:19.12 SPR analysis completed @18:05:21.35 Ended log spr23may0216h.spr.bz2 @18:05:21.37 SPR.command "stats_off" @18:05:22.08 Handoff.select :close @18:05:22.21
Gate.power :heat,:OFF @18:05:22.22 Gate.power :cartridge,:OFF @18:05:22.31 PinPort.in :unlocking @18:05:22.37 PinPort.seek :clear @18:05:22.84 Carousel.in :default @18:05:22.97 Carousel.home.seek 24 @18:05:23.34 Plunge.seek :locked @18:05:23.51 Archive.in :unlocking @18:05:23.66 Archive.seek :clear @18:05:23.73 Air.in :unlocking @18:05:23.91 Air.seek :clear @18:05:24.04 Lysis1.in :unlocking @18:05:24.15 Lysis1.seek :clear @18:05:24.23 Lysis2.in :unlocking @18:05:24.34 Lysis2.seek :clear @18:05:28.92
Gate.power :motor,:OFF @18:05:28.94 Gate.power :core,:OFF @18:05:28.95 Gate.power :analytic1,:OFF @18:05:28.96 Gate.power :analytic2,:OFF @18:05:28.98 Gate.power :analytic3,:OFF @18:05:28.99 Gate.power :main,:OFF @18:05:29.00 Safely stopped and ready to power off @18:05:29.51 Cartridge::None in MAIN -- Not enough standard cartridges remain @11:51:09.46PDT03-May-23
>BREAK< ESP3 Gateway v1.42 for core bus @11:51:09.49 >BREAK< ESP3 Gateway v1.42 for cartridge bus @11:51:12.79 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @11:51:13.41 Sea pressure (Abs) tare is 6% of full scale @11:51:21.57 Polling Can every 2:00 @11:51:21.62 Loading /home/esp/esp2/bin/processAll mission @11:51:21.83 Sea pressure (Abs) tare is 6% of full scale @11:51:21.85 Sampling default goal volumes @11:51:21.87 Options: {extraDilutions: [1,1,1]} Cartridge group: standard Spares: {spare: 0} @11:51:22.01 Gate.power :analytic1,:ON,limit to 5000mA @11:51:22.02 Gate.power :analytic2,:ON,limit to 5000mA @11:51:22.04 Gate.power :analytic3,:ON,limit to 5000mA @11:51:22.75 Archive.configure :default @11:51:22.82 SPR Module 1, FW Rev. B, FPGA Rev. C @11:51:22.95
Air.configure :default @11:51:23.01 Air.hold @11:51:23.08 Air.configure :default @11:51:23.24 Lysis1.configure :default @11:51:23.30 Lysis1.hold @11:51:23.37 Lysis1.configure :default @11:51:23.50 Lysis2.configure :default @11:51:23.65 Plunge.configure :default @11:51:23.71 Plunge.hold @11:51:23.78 Plunge.configure :default @11:51:23.93 SamplePump.configure :default @11:51:24.09 Toroid.configure :default @11:51:24.15 Toroid.hold @11:51:24.65 Toroid.configure :default @11:51:24.81 Carousel.configure :default @11:51:24.87 Carousel.hold @11:51:24.94 Carousel.configure :default @11:51:25.09 Pressure.configure :default @11:51:25.27 ExtraPump.configure :default @11:51:25.40 PinPort.configure :default @11:51:25.45 PinPort.hold @11:51:25.52 PinPort.configure :default @11:51:25.59 All elf controllers running firmware version 4.83 @11:51:26.40 Toroid.seek :clear @11:51:26.48 Carousel.in :default @11:51:26.62 Carousel.home.seek 25 @11:51:26.87 Selecting Cartridge 25 @11:51:27.19 Carousel.seek 25 @11:51:27.44 Lysis1.in :fast @11:51:27.50 Lysis1.seek :locked @11:51:30.12 Plunge.seek :locked @11:51:30.29 Air.in :fast @11:51:30.35 Air.seek :locked @11:51:31.93
Carousel.reconfigure :locked @11:51:32.09 Powering cartridge bus @11:51:34.75 Gate.setIR 1750,5000,0,6 @11:51:34.88 Gate.setIR 0,5000,0,6 @11:51:34.95 PuckHeater.configure :default @11:51:35.01 Gate.power :heat,:ON,limit to 5000mA @11:51:35.66 Mounted lysis Cartridge @11:51:35.69 WARNING: Using default puck temperature sensor calibration @11:51:35.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:51:37.24 SPR.command "read_sequences" @11:51:37.85 SPR.command "stats_on" @11:51:38.01 SPR.command "start 0 1" @11:51:38.71 SPR.start /var/log/esp/spr23may0311h.spr.bz2 @11:51:40.70 Waiting for baseline SPR stabilization @11:51:40.72 Handoff.select :waste @11:51:47.41 Syringe tare is 17% of full scale @11:51:47.44 PinPort.in :locking @11:51:47.50 PinPort.seek :locked @11:51:47.98 Archive.in :fast @11:51:48.08 Lysis2.in :fast @11:51:48.14 Archive.seek :full @11:51:48.19 Lysis1.seek 1000uL @11:51:48.33 Lysis2.seek 1000uL @11:51:52.84 delayUntil "12:01:40.25" @11:56:42.07 Session Begun @11:56:53.48 -> MainThread.exit # @11:56:54.99 Ended log spr23may0311h.spr.bz2 @11:56:55.01
SPR.command "stats_off" @11:56:56.00 Exhaust.close @11:56:56.07 Handoff.select :close @11:56:56.12 Intake.close @11:56:56.16 Bypass.close @11:56:56.56 Session Ended