@10:42:16.96PDT10-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @10:42:17.01 >BREAK< ESP3 Gateway v1.42 for cartridge bus @10:42:19.68 Configuration for honu by methane@mbari.org 1/24/23 /home/esp/esp2/type/auv/honu/configure.rb @10:42:20.15 Sea pressure (Abs) tare is 6% of full scale @10:42:24.19 Polling Can every 2:00 @10:42:24.70 Clients may connect on TCP port 9999 @10:42:24.72 Connecting to 10.89.5.2:9999 @10:42:24.76 Waiting for espclients... @10:42:25.18 Session Begun @10:42:27.11 -> showStatus true @10:42:29.14 -> Cmd.loadCartridge -10 @10:42:29.21 Gate.power :main,:ON,limit to 5000mA @10:42:29.23 Gate.power :analytic1,:ON,limit to 5000mA @10:42:29.25 Gate.power :analytic2,:ON,limit to 5000mA @10:42:29.27 Gate.power :analytic3,:ON,limit to 5000mA @10:42:29.41 Powering core bus @10:42:31.68 SPR Module 1, FW Rev. B, FPGA Rev. C @10:42:32.11 Archive.configure :default @10:42:32.20 Air.configure :default @10:42:32.27 Lysis1.configure :default @10:42:32.34 Lysis2.configure :default @10:42:32.43 Plunge.configure :default @10:42:32.52 SamplePump.configure :default @10:42:32.61 Toroid.configure :default @10:42:32.71 Carousel.configure :default @10:42:32.80 Pressure.configure :default @10:42:32.89 ExtraPump.configure :default @10:42:32.97 PinPort.configure :default @10:42:33.04 All elf controllers running firmware version 4.83 @10:42:33.07 Gate.power :motor,:ON,limit to 5000mA @10:42:33.27 Archive.home.jog 0 @10:42:33.80 Carousel.home.jog 0 @10:42:34.18 Toroid.home.jog 0 @10:42:34.23 Lysis1.home.jog 0 @10:42:34.28 Lysis2.home.jog 0 @10:42:34.32 PinPort.home.jog 0 @10:42:34.35 Air.home.jog 0 @10:42:34.39 Plunge.home.jog 0 @10:42:34.94 Archive.in :homing @10:42:35.27 Plunge.in :homing @10:42:35.28 Archive.jog 1391 @10:42:35.31 PinPort.in :homing @10:42:35.35 Toroid.in :homing @10:42:35.39 Lysis2.in :homing @10:42:35.44 Lysis1.in :homing @10:42:35.48 Air.in :homing @10:42:35.73 PinPort.jog -106 @10:42:35.76 Plunge.jog 122 @10:42:35.79 Toroid.jog 622 @10:42:35.82 Lysis2.jog 1398 @10:42:35.85 Air.jog 1379 @10:42:35.90 Lysis1.jog 1398 @10:42:36.14 PinPort.setRawPosition! 0 @10:42:36.19 PinPort.seek :home @10:42:36.27 PinPort.in :default @10:42:36.35 Plunge.setRawPosition! 50 @10:42:36.42 Plunge.seek :home @10:42:36.71 Archive.setRawPosition! 50 @10:42:36.74 Plunge.in :default @10:42:36.82 Archive.seek :home @10:42:37.12 Archive.in :default @10:42:37.22 Lysis2.setRawPosition! 50 @10:42:37.25 Air.setRawPosition! 50 @10:42:37.32 Lysis2.seek :home @10:42:37.36 Air.seek :home @10:42:37.50 Lysis1.setRawPosition! 50 @10:42:37.55 Lysis1.seek :home @10:42:37.68 Lysis2.in :default @10:42:37.75 Air.in :default @10:42:37.86 Lysis1.in :default @10:42:38.07 Toroid.setRawPosition! 0 @10:42:38.13 Toroid.seek :home @10:42:38.21 Toroid.in :default @10:42:38.29 Toroid.seek :clear @10:42:39.91 Carousel.in :default @10:42:40.02 Carousel.home.seek 46 @10:42:40.28 Selecting Cartridge 49 @10:42:40.55 Carousel.seek 49 @10:42:41.45 Lysis1.in :fast @10:42:41.52 Lysis1.seek :locked @10:42:44.13 Plunge.seek :locked @10:42:44.27 Air.in :fast @10:42:44.33 Air.seek :locked @10:42:45.91 Carousel.reconfigure :locked @10:42:46.05 Powering cartridge bus @10:42:48.66 Gate.setIR 1750,5000,0,6 @10:42:48.78 Gate.setIR 0,5000,0,6 @10:42:48.85 PuckHeater.configure :default @10:42:48.91 Gate.power :heat,:ON,limit to 5000mA @10:42:49.97 Mounted lysis Cartridge board#Q05053 @10:42:50.02 Using default cartridge heat loss curve for HeatingAccelInCarousel 49 @10:43:06.72 -> Cmd.startFiltering @10:43:06.97 Preparing to filter cartridge 49 92.07 @10:43:07.28 Priming sample loop w/100ml, bypass w/10ml @10:43:07.29 SamplePump.setPosition! 0ml @10:43:07.35 Bypass.open @10:43:08.80 Intake.open @10:43:09.55 Sea 6.2m deep (23.9psia) @10:43:09.58 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @10:43:12.15 Vacuum tare is 59% of full scale @10:43:12.29 Pressure.in :default @10:43:12.36 Pressure.hold @10:43:12.55 SamplePump.in :prime @10:43:12.62 SamplePump.seek 10ml,59 seconds @10:43:21.37 Pressure.stop @10:43:21.43 SamplePump.setPosition! 0ml @10:43:21.48 Exhaust.open @10:43:22.94 Bypass.close @10:43:24.46 Pressure.in :default @10:43:24.53 Pressure.hold @10:43:24.65 Sea 6.2m deep (23.9psia) @10:43:24.80 SamplePump.in :prime @10:43:24.87 SamplePump.seek 100ml,5:16 @10:45:01.92 Pressure.stop @10:45:01.99 Bypass.open @10:45:02.68 Intake.close @10:45:02.86 Toroid.in :hold @10:45:02.92 Toroid.seek :crack @10:45:04.46 Toroid.seek :cartridge @10:45:05.56 Toroid.in :default @10:45:05.63 Toroid.seek :clear @10:45:07.61 Pressurizing cartridge to 26psi above 23.3psia @10:45:07.62 SamplePump.setPosition! 0ml @10:45:07.68 Intake.open @10:45:08.36 Bypass.close @10:45:09.05 Exhaust.close @10:45:09.89 Pressure.in :default @10:45:09.95 Pressure.hold @10:45:10.15 SamplePump.in :charge @10:45:10.22 SamplePump.seek 2.3ml,42 seconds @10:45:20.99 Pressure.stop @10:45:21.04 Intake.close @10:45:22.17 Pressurized cartridge with 0.549ml to 26.0psi @10:45:22.18 Isolated @45.8psia (22.6psi) @10:45:22.20 Verifying cartridge holds at least 0.65..0.85 of 45.8psia @10:45:22.24 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @10:45:26.90 Sampling 1000.0ml in 1:25:20 @10:45:26.91 SamplePump.setPosition! 0ml @10:45:26.98 Exhaust.open @10:45:27.72 Intake.open @10:45:28.56 Pressure.in :default @10:45:28.62 Pressure.hold @10:45:28.75 Sea 6.1m deep (23.7psia) @10:45:28.76 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @10:45:28.92 SamplePump.in :sample @10:45:28.99 SamplePump.seek 1000ml,1:23:51 @10:45:31.20 Flow estimate reduced to 0.69ml/s @10:45:31.25 Waiting up to 26 seconds for vacuum to reduce from 33.0 to 18.0psi @10:45:31.64 Cartridge 49 filtering 1/1000ml at 0.00ml/s with 10.2psi vacuum 4.9m deep (21.9psia) @10:45:32.08 Relieved excess vacuum in .9 seconds @10:45:32.11 SamplePump.seek 1000ml,1:23:48 @10:45:33.92 Flow estimate reduced to 0.55ml/s @10:45:33.95 Waiting up to 18 seconds for vacuum to reduce from 30.1 to 18.0psi @10:45:34.54 Relieved excess vacuum in .6 seconds @10:45:34.57 SamplePump.seek 1000ml,1:23:46 @10:45:36.36 Flow estimate reduced to 0.44ml/s @10:45:36.39 Waiting up to 16 seconds for vacuum to reduce from 29.3 to 18.0psi @10:45:37.23 Relieved excess vacuum in .9 seconds @10:45:37.26 SamplePump.seek 1000ml,1:23:45 @10:45:39.08 Flow estimate reduced to 0.35ml/s @10:45:39.11 Waiting up to 20 seconds for vacuum to reduce from 30.6 to 18.0psi @10:45:39.96 Relieved excess vacuum in .9 seconds @10:45:39.98 SamplePump.seek 1000ml,1:23:43 @10:45:41.79 Flow estimate reduced to 0.28ml/s @10:45:41.83 Waiting up to 20 seconds for vacuum to reduce from 30.9 to 18.0psi @10:45:42.67 Relieved excess vacuum in .9 seconds @10:45:42.70 SamplePump.seek 1000ml,1:23:41 @10:45:44.50 Flow estimate reduced to 0.23ml/s @10:45:44.55 Waiting up to 18 seconds for vacuum to reduce from 29.9 to 18.0psi @10:45:45.14 Relieved excess vacuum in .6 seconds @10:45:45.16 SamplePump.seek 1000ml,1:23:40 @10:45:46.94 Flow estimate reduced to 0.18ml/s @10:45:46.96 Intake.close @10:45:47.04 Exhaust.close @10:45:47.69 Toroid.in :hold @10:45:47.78 Toroid.seek :bypass @10:45:49.44 Toroid.in :default @10:45:49.52 Toroid.seek :clear @10:45:51.14 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @10:45:51.16 Pressure.stop @10:45:51.36 Sampled 2.5ml @10:45:53.56 -> Cmd.startProcessing 120.0 @10:45:55.04 SPR.command "read_sequences" @10:45:55.08 SPR.command "stats_on" @10:45:55.94 SPR.command "start 0 1" @10:45:56.65 SPR.start /var/log/esp/spr23apr1010h.spr.bz2 @10:45:58.69 Handoff.select :waste @10:46:05.38 Syringe tare is 17% of full scale @10:46:05.40 PinPort.in :locking @10:46:05.49 PinPort.seek :locked @10:46:05.97 Archive.in :fast @10:46:06.02 Lysis2.in :fast @10:46:06.10 Lysis1.seek 1350uL @10:46:06.21 Archive.seek :full @10:46:06.27 Lysis2.seek :full @10:46:08.28 Plunge.seek :reagent @10:46:09.19 Evacuating puck with filter bubble point of 50psi @10:46:09.21 PinPort.seek :locked @10:46:09.31 Handoff.select :waste @10:46:15.89 Air syringe pumping up to 1 strokes to reach 8psi @10:46:15.91 Air stroke #1 of 1 @10:46:16.01 Air.in :pressurize @10:46:16.08 Air.seek :pressurized @10:46:21.82 1.4psi after pressurize @10:46:21.85 Air.in :compress @10:46:21.96 Air.seek :evac @10:46:24.97 1.3psi after compress @10:46:36.06 -0.1psi after compress (stable) @10:46:36.09 Air.in :default @10:46:36.18 Air.hold @10:46:36.36 at -0.1psi Opened to waste @10:46:36.37 Handoff.select :blocked @10:46:36.52 Archive syringe pumping up to 8 strokes to reach 50psi @10:46:36.53 Archive stroke #1 of 8 @10:46:36.62 Archive.in :pressurize @10:46:36.69 Archive.seek :pressurized @10:46:49.21 -0.0psi after pressurize @10:47:18.29 9.8psi after pressurize (stable) @10:47:18.31 Archive.in :compress @10:47:18.39 Archive.seek :empty @10:47:33.05 10.1psi after compress @10:47:43.62 10.3psi after compress (stable) @10:47:43.64 Archive.in :gulp @10:47:43.72 Archive.seek :topVent @10:47:49.98 10.6psi after gulp @10:47:55.56 10.7psi after gulp (stable) @10:47:55.57 Archive stroke #2 of 8 @10:47:55.67 Archive.in :pressurize @10:47:55.74 Archive.seek :pressurized @10:48:08.26 10.8psi after pressurize @10:48:13.83 10.8psi after pressurize (stable) @10:48:13.85 Archive.in :compress @10:48:13.94 Archive.seek :empty @10:48:28.62 11.6psi after compress @10:48:53.72 15.1psi after compress (stable) @10:48:53.74 Archive.in :gulp @10:48:53.82 Archive.seek :topVent @10:49:00.07 16.2psi after gulp @10:49:05.66 16.6psi after gulp (stable) @10:49:05.67 Archive stroke #3 of 8 @10:49:05.78 Archive.in :pressurize @10:49:05.86 Archive.seek :pressurized @10:49:18.37 17.4psi after pressurize @10:49:23.94 17.8psi after pressurize (stable) @10:49:23.96 Archive.in :compress @10:49:24.05 Archive.seek :empty @10:49:38.68 24.4psi after compress @10:50:02.28 36.4psi after compress (stable) @10:50:02.32 Archive.in :gulp @10:50:02.39 Archive.seek :topVent @10:50:08.64 37.2psi after gulp @10:50:14.22 37.8psi after gulp (stable) @10:50:14.23 Archive stroke #4 of 8 @10:50:14.33 Archive.in :pressurize @10:50:14.40 Archive.seek :pressurized @10:50:26.92 38.8psi after pressurize @10:50:32.51 39.3psi after pressurize (stable) @10:50:32.55 Archive.in :compress @10:50:32.64 Archive.seek :empty @10:50:47.28 42.1psi after compress @10:51:04.51 Reached 50.0 of 50.0psi after pumping 6920uL (4.00 strokes) @10:51:04.52 Archive.in :default @10:51:04.60 Archive.hold @10:51:04.70 Handoff.select :waste @10:51:20.40 delay 1:00 @10:52:20.42 Handoff.select :blocked @10:52:43.10 Handoff.select :waste @10:52:43.23 1.0psi at evacEnd @10:52:43.24 Handoff.select :blocked @10:52:43.37 Archive.in :fast @10:52:43.44 Archive.seek :topVent @10:52:57.12 Stable @0.8psi after Handoff blocked @10:52:57.14 Archive syringe pumping up to 8 strokes to reach 31psi @10:52:57.16 Archive stroke #1 of 8 @10:52:57.30 Archive.in :pressurize @10:52:57.38 Archive.seek :pressurized @10:53:09.88 1.1psi after pressurize @10:53:09.89 Archive.in :compress @10:53:09.98 Archive.seek :empty @10:53:24.63 5.0psi after compress @10:53:49.23 12.9psi after compress (stable) @10:53:49.26 Archive.in :gulp @10:53:49.35 Archive.seek :topVent @10:53:55.62 13.6psi after gulp @10:54:01.19 13.9psi after gulp (stable) @10:54:01.20 Archive stroke #2 of 8 @10:54:01.30 Archive.in :pressurize @10:54:01.37 Archive.seek :pressurized @10:54:13.91 14.5psi after pressurize @10:54:13.93 Archive.in :compress @10:54:14.01 Archive.seek :empty @10:54:28.65 19.3psi after compress @10:54:52.22 27.8psi after compress (stable) @10:54:52.25 Archive.in :gulp @10:54:52.33 Archive.seek :topVent @10:54:58.58 28.6psi after gulp @10:55:04.17 28.8psi after gulp (stable) @10:55:04.18 Archive stroke #3 of 8 @10:55:04.29 Archive.in :pressurize @10:55:04.36 Archive.seek :pressurized @10:55:16.86 29.2psi after pressurize @10:55:16.88 Archive.in :compress @10:55:16.97 Archive.seek :empty @10:55:29.61 Reached 31.0 of 30.8psi after pumping 5145uL (2.97 strokes) @10:55:29.62 Archive.in :default @10:55:29.70 Archive.hold @10:55:29.77 Handoff.select :waste @10:55:37.74 delay 1:00 @10:56:37.82 Handoff.select :blocked @10:57:00.52 Handoff.select :waste @10:57:00.64 0.2psi at evacEnd @10:57:00.66 Air.seek :fullVent @10:57:02.09 Archive.in :fast @10:57:02.18 Archive.seek :topVent @10:57:09.15 Seawater evacuated, Pressure above/below filter released. @10:57:09.24 Lysis1.in :slow @10:57:09.31 Lysis1.seek 1050uL @10:57:39.36 delay 5 seconds @10:57:44.38 Handoff.select :blocked @10:57:44.56 Lysis1.seek 450uL @10:58:44.58 delay 3 seconds @10:58:47.66 Air.in mix with 1.40048 parts Lysis1 @10:58:48.16 Air.seek :evac @10:58:48.25 Lysis1.seek 300uL @10:59:03.33 Lysis1.seek :empty @10:59:33.45 Lysis buffer delivered @18.0psi @10:59:33.47 Polling Can every 2:00 @10:59:34.03 PuckHeater.configure :default #ambient 11.6C @10:59:34.08 PuckHeater.seek 80.2C @11:00:46.27 PuckHeater.hold 2:00,80.2C @11:02:46.20 Polling Can every 2:00 @11:02:46.74 PuckHeater.configure :default #ambient 11.7C @11:02:46.80 PuckHeater.hold 30 seconds,79.2C @11:03:16.94 Polling Can every 2:00 @11:03:17.48 PuckHeater.configure :default #ambient 11.7C @11:03:17.53 PuckHeater.hold 30 seconds,78.6C @11:03:47.67 Polling Can every 2:00 @11:03:48.21 PuckHeater.configure :default #ambient 11.7C @11:03:48.28 PuckHeater.hold 10:00,78.1C @11:13:47.31 Lysis complete. Syringe pressure @20.5psi @11:13:47.33 Archive syringe pumping up to 5 strokes to reach 40psi @11:13:47.35 Archive stroke #1 of 5 @11:13:47.45 Archive.in :pressurize @11:13:47.52 Archive.seek :pressurized @11:14:00.02 20.1psi after pressurize @11:14:05.62 19.9psi after pressurize (stable) @11:14:05.65 Archive.in :compress @11:14:05.73 Archive.seek :empty @11:14:13.86 Reached 40.7 of 40.0psi after pumping 1590uL (0.92 strokes) @11:14:13.87 Archive.in :default @11:14:13.95 Archive.hold @11:14:14.02 delay 2 seconds @11:14:16.05 Archive.in :fast @11:14:16.12 Archive.seek :full @11:14:22.80 Lysis1.seek 138uL @11:14:36.68 delay 1 second @11:14:38.07 Archive.seek :full @11:14:38.11 Lysis1.seek :empty @11:14:52.03 Handoff.select :blocked @11:14:52.12 Initial air pressure @44.1psi @11:14:52.20 Lysis1.in :extract @11:14:52.28 Lysis1.seek 600uL,nil @11:14:55.11 Archive syringe pumping up to 6 strokes to reach 25psi @11:14:55.12 Archive stroke #1 of 6 @11:14:55.22 Archive.in :pressurize @11:14:55.31 Archive.seek :pressurized @11:15:07.82 14.3psi after pressurize @11:15:13.40 14.2psi after pressurize (stable) @11:15:13.41 Archive.in :compress @11:15:13.51 Archive.seek :empty @11:15:20.60 Archive.in :default @11:15:20.66 Archive.hold @11:15:20.81 Initial 600uL lysate @17.4psi @11:15:20.82 Handoff.select :blocked @11:15:20.92 Initial air pressure @17.9psi @11:15:20.98 Lysis1.seek 825uL,nil @11:15:22.67 Archive syringe pumping up to 6 strokes to reach 17psi @11:15:22.68 Archive stroke #1 of 6 @11:15:22.78 Archive.in :compress @11:15:22.86 Archive.seek :empty @11:15:25.48 Archive.in :default @11:15:25.54 Archive.hold @11:15:25.76 Recovered 825uL lysate @14.8psi @11:15:25.78 Air.in :halfSpeed @11:15:25.86 Air.seek :fullVent @11:15:28.06 Puck Top Vented, bottom @9.0psi @11:15:28.07 Handoff.select :waste @11:15:28.21 delay 5 seconds @11:15:33.27 Handoff open to waste, bottom @0.1psi @11:15:33.29 Air.in :default @11:15:33.36 Air.seek :evac @11:15:34.78 Lysis2.in :DAmix @11:15:34.90 Lysis1.in :DAmix @11:15:35.34 Lysis2.seek 1480uL @11:15:35.38 Lysis1.seek 575uL @11:16:10.51 delay 5 seconds @11:16:15.53 Handoff.select SPR @11:16:15.66 SPR.command "start 2 14" @11:16:16.58 SPR stabilized @11:16:16.73 delay 10 seconds @11:16:26.75 SPR.command "start 1 0" @11:16:29.95 Waiting up to 4:00 for SPR to enter state 1 @11:18:34.20 SPR.command "hold_on" @11:18:35.63 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @11:18:36.09 Lysis2.seek 1180uL @11:18:36.18 Lysis1.seek 275uL @11:19:18.20 delay 15 seconds @11:19:33.22 SPR.command "hold_off" @11:19:36.28 Waiting up to 38:00 for SPR to enter state 7 @11:37:35.86 SPRsummary:16.5RIU,226RIU,193RIU,286RIU,none,none,none after 3274s @11:44:13.08 SPR analysis completed @11:44:19.90 Ended log spr23apr1010h.spr.bz2 @11:44:19.91 SPR.command "stats_off" @11:44:21.17 -> Cmd.stop @11:44:21.27 Handoff.select :close @11:44:21.40 Gate.power :heat,:OFF @11:44:21.42 Gate.power :cartridge,:OFF @11:44:21.51 PinPort.in :unlocking @11:44:21.58 PinPort.seek :clear @11:44:22.05 Carousel.in :default @11:44:22.17 Carousel.home.seek 49 @11:44:22.48 Plunge.seek :locked @11:44:23.36 Archive.in :unlocking @11:44:23.50 Air.in :unlocking @11:44:23.54 Archive.seek :clear @11:44:23.73 Lysis1.in :unlocking @11:44:23.77 Air.seek :clear @11:44:23.93 Lysis1.seek :clear @11:44:24.02 Lysis2.in :unlocking @11:44:24.10 Lysis2.seek :clear @11:44:28.07 Gate.power :motor,:OFF @11:44:28.09 Gate.power :core,:OFF @11:44:28.11 Gate.power :analytic1,:OFF @11:44:28.14 Gate.power :analytic2,:OFF @11:44:28.16 Gate.power :analytic3,:OFF @11:44:28.18 Gate.power :main,:OFF @11:44:28.20 Safely stopped and ready to power off :STOPPED