@08:57:01.07PDT16-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @08:57:01.11 >BREAK< ESP3 Gateway v1.42 for cartridge bus @08:57:03.69 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @08:57:04.21 Sea pressure (Abs) tare is 6% of full scale @08:57:08.86 Polling Can every 2:00 @08:57:09.37 Clients may connect on TCP port 9999 @08:57:09.40 Connecting to 10.89.5.2:9999 @08:57:09.43 Waiting for espclients... @08:57:09.74 Session Begun @08:57:11.67 -> showStatus true @08:57:13.69 -> Cmd.loadCartridge -10 @08:57:13.76 Gate.power :main,:ON,limit to 5000mA @08:57:13.77 Gate.power :analytic1,:ON,limit to 5000mA @08:57:13.79 Gate.power :analytic2,:ON,limit to 5000mA @08:57:13.82 Gate.power :analytic3,:ON,limit to 5000mA @08:57:13.95 Powering core bus @08:57:16.25 SPR Module 1, FW Rev. B, FPGA Rev. C @08:57:16.75 Archive.configure :default @08:57:16.83 Air.configure :default @08:57:16.90 Lysis1.configure :default @08:57:16.97 Lysis2.configure :default @08:57:17.06 Plunge.configure :default @08:57:17.15 SamplePump.configure :default @08:57:17.24 Toroid.configure :default @08:57:17.33 Carousel.configure :default @08:57:17.42 Pressure.configure :default @08:57:17.50 ExtraPump.configure :default @08:57:17.57 PinPort.configure :default @08:57:17.65 All elf controllers running firmware version 4.83 @08:57:17.67 Gate.power :motor,:ON,limit to 5000mA @08:57:17.92 Archive.home.jog 0 @08:57:18.36 Carousel.home.jog 0 @08:57:18.42 Toroid.home.jog 0 @08:57:18.44 Lysis2.home.jog 0 @08:57:18.48 Lysis1.home.jog 0 @08:57:18.51 PinPort.home.jog 0 @08:57:18.56 Air.home.jog 0 @08:57:18.60 Plunge.home.jog 0 @08:57:19.12 Archive.in :homing @08:57:19.24 Archive.jog 1393 @08:57:19.42 Toroid.in :homing @08:57:19.45 Plunge.in :homing @08:57:19.48 PinPort.in :homing @08:57:19.52 Lysis2.in :homing @08:57:19.56 Lysis1.in :homing @08:57:19.60 Air.in :homing @08:57:19.82 Plunge.jog 1555 @08:57:19.85 PinPort.jog -105 @08:57:19.93 Toroid.jog 631 @08:57:19.96 Lysis2.jog 1407 @08:57:19.99 Lysis1.jog 161 @08:57:20.04 Air.jog 1374 @08:57:20.31 PinPort.setRawPosition! 0 @08:57:20.36 PinPort.seek :home @08:57:20.46 Archive.setRawPosition! 50 @08:57:20.50 PinPort.in :default @08:57:20.54 Archive.seek :home @08:57:20.67 Lysis1.setRawPosition! 50 @08:57:20.71 Lysis1.seek :home @08:57:20.85 Archive.in :default @08:57:20.99 Lysis1.in :default @08:57:21.25 Plunge.setRawPosition! 50 @08:57:21.30 Plunge.seek :home @08:57:21.40 Air.setRawPosition! 50 @08:57:21.42 Lysis2.setRawPosition! 50 @08:57:21.50 Air.seek :home @08:57:21.54 Lysis2.seek :home @08:57:21.67 Plunge.in :default @08:57:21.84 Air.in :default @08:57:21.91 Lysis2.in :default @08:57:22.25 Toroid.setRawPosition! 0 @08:57:22.30 Toroid.seek :home @08:57:22.39 Toroid.in :default @08:57:22.46 Toroid.seek :clear @08:57:24.09 Carousel.in :default @08:57:24.20 Carousel.home.seek 38 @08:57:24.47 Selecting Cartridge 49 @08:57:25.10 Carousel.seek 49 @08:57:27.68 Lysis1.in :fast @08:57:27.74 Lysis1.seek :locked @08:57:30.36 Plunge.seek :locked @08:57:30.50 Air.in :fast @08:57:30.56 Air.seek :locked @08:57:32.13 Carousel.reconfigure :locked @08:57:32.28 Powering cartridge bus @08:57:34.90 Gate.setIR 1750,5000,0,6 @08:57:35.02 Gate.setIR 0,5000,0,6 @08:57:35.09 PuckHeater.configure :default @08:57:35.15 Gate.power :heat,:ON,limit to 5000mA @08:57:35.84 Mounted lysis Cartridge board#Q05054 @08:57:35.87 Using default cartridge heat loss curve for HeatingAccelInCarousel 49 @08:57:51.26 -> Cmd.startFiltering @08:57:51.51 Preparing to filter cartridge 49 92.07 @08:57:51.79 Priming sample loop w/100ml, bypass w/10ml @08:57:51.81 SamplePump.setPosition! 0ml @08:57:51.86 Bypass.open @08:57:53.37 Intake.open @08:57:54.13 Sea 7.8m deep (26.2psia) @08:57:54.16 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:57:56.74 Vacuum tare is 60% of full scale @08:57:56.88 Pressure.in :default @08:57:56.94 Pressure.hold @08:57:57.13 SamplePump.in :prime @08:57:57.20 SamplePump.seek 10ml,59 seconds @08:58:05.96 Pressure.stop @08:58:06.02 SamplePump.setPosition! 0ml @08:58:06.08 Exhaust.open @08:58:07.50 Bypass.close @08:58:09.02 Pressure.in :default @08:58:09.08 Pressure.hold @08:58:09.20 Sea 7.5m deep (25.8psia) @08:58:09.35 SamplePump.in :prime @08:58:09.42 SamplePump.seek 100ml,5:16 @08:59:46.91 Pressure.stop @08:59:46.99 Bypass.open @08:59:47.71 Intake.close @08:59:48.49 Toroid.in :hold @08:59:48.55 Toroid.seek :crack @08:59:50.08 Toroid.seek :cartridge @08:59:51.20 Toroid.in :default @08:59:51.26 Toroid.seek :clear @08:59:53.23 Pressurizing cartridge to 26psi above 25.6psia @08:59:53.25 SamplePump.setPosition! 0ml @08:59:53.30 Intake.open @08:59:53.96 Bypass.close @08:59:54.67 Exhaust.close @08:59:55.49 Pressure.in :default @08:59:55.55 Pressure.hold @08:59:55.78 SamplePump.in :charge @08:59:55.85 SamplePump.seek 2.3ml,42 seconds @09:00:05.76 Pressure.stop @09:00:05.82 Intake.close @09:00:06.03 Pressurized cartridge with 0.464ml to 26.5psi @09:00:06.04 Isolated @50.0psia (24.4psi) @09:00:06.06 Verifying cartridge holds at least 0.65..0.85 of 50.0psia @09:00:06.10 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @09:00:10.68 Cartridge pressure fell 5% to 47.5psia @09:00:10.78 Sampling 1000.0ml in 1:25:20 @09:00:10.80 SamplePump.setPosition! 0ml @09:00:10.86 Exhaust.open @09:00:11.61 Intake.open @09:00:12.48 Pressure.in :default @09:00:12.55 Pressure.hold @09:00:12.67 Sea 7.4m deep (25.6psia) @09:00:12.68 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @09:00:12.84 SamplePump.in :sample @09:00:12.91 SamplePump.seek 1000ml,1:23:51 @09:00:15.52 Cartridge 49 filtering 1/1000ml at 0.38ml/s with 25.1psi vacuum 6.4m deep (24.1psia) @09:01:15.53 Cartridge 49 filtering 23/1000ml at 0.38ml/s with 21.4psi vacuum 7.1m deep (25.2psia) @09:02:15.53 Cartridge 49 filtering 45/1000ml at 0.38ml/s with 22.6psi vacuum 7.8m deep (26.3psia) @09:03:15.52 Cartridge 49 filtering 67/1000ml at 0.35ml/s with 25.1psi vacuum 8.2m deep (26.8psia) @09:04:15.53 Cartridge 49 filtering 88/1000ml at 0.35ml/s with 26.0psi vacuum 6.2m deep (23.8psia) @09:05:15.52 Cartridge 49 filtering 108/1000ml at 0.33ml/s with 21.2psi vacuum 7.7m deep (26.1psia) @09:06:15.53 Cartridge 49 filtering 129/1000ml at 0.35ml/s with 26.2psi vacuum 7.0m deep (25.0psia) @09:07:15.52 Cartridge 49 filtering 148/1000ml at 0.34ml/s with 24.5psi vacuum 5.7m deep (23.1psia) @09:08:15.52 Cartridge 49 filtering 168/1000ml at 0.34ml/s with 22.0psi vacuum 7.1m deep (25.1psia) @09:09:15.52 Cartridge 49 filtering 187/1000ml at 0.34ml/s with 22.7psi vacuum 6.7m deep (24.6psia) @09:10:15.53 Cartridge 49 filtering 206/1000ml at 0.32ml/s with 19.8psi vacuum 7.7m deep (26.0psia) @09:11:15.52 Cartridge 49 filtering 224/1000ml at 0.30ml/s with 21.6psi vacuum 7.4m deep (25.6psia) @09:12:15.90 Cartridge 49 filtering 242/1000ml at 0.28ml/s with 22.9psi vacuum 4.8m deep (21.8psia) @09:13:15.53 Cartridge 49 filtering 260/1000ml at 0.27ml/s with 24.5psi vacuum 5.5m deep (22.8psia) @09:14:15.53 Cartridge 49 filtering 277/1000ml at 0.30ml/s with 19.7psi vacuum 6.8m deep (24.7psia) @09:15:15.53 Cartridge 49 filtering 295/1000ml at 0.26ml/s with 26.1psi vacuum 3.1m deep (19.2psia) @09:16:15.53 Cartridge 49 filtering 312/1000ml at 0.30ml/s with 27.7psi vacuum 4.7m deep (21.7psia) @09:17:15.52 Cartridge 49 filtering 329/1000ml at 0.28ml/s with 27.1psi vacuum 4.3m deep (21.1psia) @09:18:15.52 Cartridge 49 filtering 345/1000ml at 0.27ml/s with 25.4psi vacuum 7.2m deep (25.3psia) @09:19:15.53 Cartridge 49 filtering 362/1000ml at 0.30ml/s with 24.2psi vacuum 4.9m deep (22.0psia) @09:20:15.53 Cartridge 49 filtering 378/1000ml at 0.27ml/s with 22.4psi vacuum 7.1m deep (25.1psia) @09:21:15.53 Cartridge 49 filtering 394/1000ml at 0.28ml/s with 21.3psi vacuum 7.9m deep (26.4psia) @09:22:15.52 Cartridge 49 filtering 409/1000ml at 0.25ml/s with 25.0psi vacuum 6.5m deep (24.3psia) @09:23:15.52 Cartridge 49 filtering 425/1000ml at 0.26ml/s with 23.2psi vacuum 7.0m deep (25.0psia) @09:24:15.53 Cartridge 49 filtering 440/1000ml at 0.26ml/s with 22.2psi vacuum 5.0m deep (22.1psia) @09:25:15.53 Cartridge 49 filtering 455/1000ml at 0.27ml/s with 21.4psi vacuum 8.3m deep (26.9psia) @09:26:15.52 Cartridge 49 filtering 470/1000ml at 0.23ml/s with 23.5psi vacuum 4.2m deep (20.8psia) @09:27:15.52 Cartridge 49 filtering 485/1000ml at 0.23ml/s with 23.4psi vacuum 5.9m deep (23.4psia) @09:28:15.53 Cartridge 49 filtering 500/1000ml at 0.24ml/s with 24.0psi vacuum 6.0m deep (23.6psia) @09:29:15.53 Cartridge 49 filtering 515/1000ml at 0.22ml/s with 23.8psi vacuum 5.4m deep (22.7psia) @09:30:15.53 Cartridge 49 filtering 529/1000ml at 0.24ml/s with 24.5psi vacuum 7.3m deep (25.5psia) @09:31:15.52 Cartridge 49 filtering 544/1000ml at 0.25ml/s with 24.2psi vacuum 5.9m deep (23.4psia) @09:32:15.52 Cartridge 49 filtering 558/1000ml at 0.24ml/s with 24.0psi vacuum 5.3m deep (22.5psia) @09:33:15.52 Cartridge 49 filtering 572/1000ml at 0.23ml/s with 24.4psi vacuum 7.0m deep (25.0psia) @09:34:15.53 Cartridge 49 filtering 586/1000ml at 0.22ml/s with 23.6psi vacuum 5.5m deep (22.8psia) @09:35:15.52 Cartridge 49 filtering 600/1000ml at 0.21ml/s with 27.3psi vacuum 4.2m deep (20.9psia) @09:36:15.53 Cartridge 49 filtering 614/1000ml at 0.23ml/s with 23.6psi vacuum 4.8m deep (21.8psia) @09:37:15.52 Cartridge 49 filtering 627/1000ml at 0.25ml/s with 23.8psi vacuum 6.1m deep (23.7psia) @09:38:15.53 Cartridge 49 filtering 641/1000ml at 0.21ml/s with 23.8psi vacuum 6.1m deep (23.7psia) @09:39:15.52 Cartridge 49 filtering 654/1000ml at 0.24ml/s with 23.8psi vacuum 6.1m deep (23.7psia) @09:40:15.53 Cartridge 49 filtering 667/1000ml at 0.24ml/s with 24.1psi vacuum 6.2m deep (23.8psia) @09:41:15.52 Cartridge 49 filtering 681/1000ml at 0.22ml/s with 22.9psi vacuum 5.8m deep (23.2psia) @09:42:15.52 Cartridge 49 filtering 694/1000ml at 0.23ml/s with 24.9psi vacuum 5.7m deep (23.0psia) @09:43:15.52 Cartridge 49 filtering 707/1000ml at 0.23ml/s with 26.1psi vacuum 6.5m deep (24.3psia) @09:44:15.53 Cartridge 49 filtering 719/1000ml at 0.21ml/s with 22.2psi vacuum 6.3m deep (24.1psia) @09:45:15.52 Cartridge 49 filtering 732/1000ml at 0.23ml/s with 25.5psi vacuum 6.9m deep (24.9psia) @09:46:15.52 Cartridge 49 filtering 745/1000ml at 0.23ml/s with 26.0psi vacuum 5.8m deep (23.3psia) @09:47:15.52 Cartridge 49 filtering 757/1000ml at 0.20ml/s with 23.2psi vacuum 6.4m deep (24.2psia) @09:48:15.53 Cartridge 49 filtering 770/1000ml at 0.20ml/s with 25.0psi vacuum 5.4m deep (22.7psia) @09:49:15.91 Cartridge 49 filtering 782/1000ml at 0.20ml/s with 25.7psi vacuum 3.1m deep (19.3psia) @09:50:15.53 Cartridge 49 filtering 795/1000ml at 0.20ml/s with 24.8psi vacuum 5.4m deep (22.6psia) @09:51:15.52 Cartridge 49 filtering 807/1000ml at 0.20ml/s with 24.7psi vacuum 4.9m deep (22.0psia) @09:52:15.53 Cartridge 49 filtering 819/1000ml at 0.20ml/s with 24.0psi vacuum 6.3m deep (24.0psia) @09:53:15.52 Cartridge 49 filtering 831/1000ml at 0.20ml/s with 27.9psi vacuum 4.7m deep (21.6psia) @09:54:15.52 Cartridge 49 filtering 844/1000ml at 0.20ml/s with 23.9psi vacuum 5.9m deep (23.5psia) @09:55:15.52 Cartridge 49 filtering 856/1000ml at 0.20ml/s with 25.9psi vacuum 5.8m deep (23.2psia) @09:56:15.56 Cartridge 49 filtering 868/1000ml at 0.20ml/s with 25.8psi vacuum 5.3m deep (22.5psia) @09:57:15.52 Cartridge 49 filtering 880/1000ml at 0.20ml/s with 23.3psi vacuum 5.6m deep (23.0psia) @09:57:29.59 Flow averaged 0.26ml/s over 57:17 @09:57:29.63 Waiting up to 9 seconds for vacuum to reduce from 26.2 to 18.0psi @09:57:30.21 Relieved excess vacuum in .6 seconds @09:57:30.24 SamplePump.seek 1000ml,10:16 @09:58:15.52 Cartridge 49 filtering 892/1000ml at 0.20ml/s with 26.0psi vacuum 5.2m deep (22.4psia) @09:59:15.52 Cartridge 49 filtering 904/1000ml at 0.20ml/s with 25.9psi vacuum 5.7m deep (23.1psia) @10:00:15.53 Cartridge 49 filtering 916/1000ml at 0.21ml/s with 22.6psi vacuum 6.9m deep (24.9psia) @10:00:50.56 Flow averaged 0.20ml/s over 3:21 @10:00:50.60 Waiting up to 10 seconds for vacuum to reduce from 26.8 to 18.0psi @10:00:51.18 Relieved excess vacuum in .6 seconds @10:00:51.21 SamplePump.seek 1000ml,6:54 @10:01:15.52 Cartridge 49 filtering 928/1000ml at 0.20ml/s with 26.3psi vacuum 5.3m deep (22.5psia) @10:02:15.53 Cartridge 49 filtering 940/1000ml at 0.20ml/s with 26.9psi vacuum 5.8m deep (23.3psia) @10:02:54.45 Flow averaged 0.20ml/s over 2:04 @10:02:54.49 Waiting up to 12 seconds for vacuum to reduce from 27.5 to 18.0psi @10:02:55.07 Relieved excess vacuum in .6 seconds @10:02:55.10 SamplePump.seek 1000ml,4:50 @10:03:15.53 Cartridge 49 filtering 952/1000ml at 0.20ml/s with 26.2psi vacuum 4.1m deep (20.8psia) @10:04:01.03 Flow averaged 0.20ml/s over 1:06 @10:04:01.07 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @10:04:01.66 Relieved excess vacuum in .6 seconds @10:04:01.68 SamplePump.seek 1000ml,3:43 @10:04:15.56 Cartridge 49 filtering 964/1000ml at 0.20ml/s with 25.2psi vacuum 6.1m deep (23.7psia) @10:05:05.95 Flow averaged 0.20ml/s over 1:05 @10:05:05.99 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @10:05:06.57 Relieved excess vacuum in .6 seconds @10:05:06.60 SamplePump.seek 1000ml,2:38 @10:05:15.52 Cartridge 49 filtering 976/1000ml at 0.20ml/s with 23.8psi vacuum 5.8m deep (23.2psia) @10:06:04.49 Flow averaged 0.20ml/s over 58 seconds @10:06:04.52 Waiting up to 10 seconds for vacuum to reduce from 26.6 to 18.0psi @10:06:05.12 Relieved excess vacuum in .6 seconds @10:06:05.15 SamplePump.seek 1000ml,1:40 @10:06:15.52 Cartridge 49 filtering 988/1000ml at 0.21ml/s with 22.3psi vacuum 7.1m deep (25.2psia) @10:07:03.09 Flow averaged 0.20ml/s over 58 seconds @10:07:03.13 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @10:07:03.73 Relieved excess vacuum in .6 seconds @10:07:03.76 SamplePump.seek 1000ml,42 seconds @10:07:15.43 Pressure.stop @10:07:15.55 Intake.close @10:07:15.61 Exhaust.close @10:07:15.78 Toroid.in :hold @10:07:15.86 Toroid.seek :bypass @10:07:17.49 Toroid.in :default @10:07:17.57 Toroid.seek :clear @10:07:19.18 Sampled 1000.0ml @10:07:20.66 -> Cmd.startProcessing 120.0 @10:07:22.12 SPR.command "read_sequences" @10:07:22.21 SPR.command "stats_on" @10:07:22.34 SPR.command "start 0 1" @10:07:23.07 SPR.start /var/log/esp/spr23apr1610h.spr.bz2 @10:07:25.06 Handoff.select :waste @10:07:31.75 Syringe tare is 16% of full scale @10:07:31.77 PinPort.in :locking @10:07:31.86 PinPort.seek :locked @10:07:32.34 Archive.in :fast @10:07:32.38 Lysis2.in :fast @10:07:32.47 Lysis1.seek 1350uL @10:07:32.54 Archive.seek :full @10:07:32.64 Lysis2.seek :full @10:07:34.63 Plunge.seek :reagent @10:07:35.52 Evacuating puck with filter bubble point of 50psi @10:07:35.54 PinPort.seek :locked @10:07:35.63 Handoff.select :waste @10:07:42.22 Air syringe pumping up to 1 strokes to reach 8psi @10:07:42.24 Air stroke #1 of 1 @10:07:42.33 Air.in :pressurize @10:07:42.41 Air.seek :pressurized @10:07:48.13 0.4psi after pressurize @10:07:48.16 Air.in :compress @10:07:48.26 Air.seek :evac @10:07:51.26 0.9psi after compress @10:08:01.84 0.0psi after compress (stable) @10:08:01.85 Air.in :default @10:08:01.92 Air.hold @10:08:02.11 at -0.0psi Opened to waste @10:08:02.12 Handoff.select :blocked @10:08:02.26 Archive syringe pumping up to 8 strokes to reach 50psi @10:08:02.27 Archive stroke #1 of 8 @10:08:02.35 Archive.in :pressurize @10:08:02.44 Archive.seek :pressurized @10:08:14.95 10.8psi after pressurize @10:08:57.08 20.9psi after pressurize (stable) @10:08:57.10 Archive.in :compress @10:08:57.17 Archive.seek :empty @10:09:11.80 29.7psi after compress @10:09:28.88 38.4psi after compress (stable) @10:09:28.90 Archive.in :gulp @10:09:28.98 Archive.seek :topVent @10:09:35.23 38.8psi after gulp @10:09:40.84 38.8psi after gulp (stable) @10:09:40.86 Archive stroke #2 of 8 @10:09:40.95 Archive.in :pressurize @10:09:41.03 Archive.seek :pressurized @10:09:53.53 38.3psi after pressurize @10:09:59.10 38.5psi after pressurize (stable) @10:09:59.17 Archive.in :compress @10:09:59.25 Archive.seek :empty @10:10:12.30 Reached 50.3 of 50.0psi after pumping 3425uL (1.98 strokes) @10:10:12.31 Archive.in :default @10:10:12.39 Archive.hold @10:10:12.46 Handoff.select :waste @10:10:21.64 delay 1:00 @10:11:21.66 Handoff.select :blocked @10:11:44.34 Handoff.select :waste @10:11:44.47 2.2psi at evacEnd @10:11:44.48 Handoff.select :blocked @10:11:44.61 Archive.in :fast @10:11:44.71 Archive.seek :topVent @10:11:58.25 Stable @2.9psi after Handoff blocked @10:11:58.27 Archive syringe pumping up to 8 strokes to reach 33psi @10:11:58.29 Archive stroke #1 of 8 @10:11:58.39 Archive.in :pressurize @10:11:58.45 Archive.seek :pressurized @10:12:10.95 4.0psi after pressurize @10:12:10.96 Archive.in :compress @10:12:11.05 Archive.seek :empty @10:12:25.66 6.1psi after compress @10:13:13.23 28.9psi after compress (stable) @10:13:13.25 Archive.in :gulp @10:13:13.33 Archive.seek :topVent @10:13:19.57 30.0psi after gulp @10:13:25.15 30.6psi after gulp (stable) @10:13:25.16 Archive stroke #2 of 8 @10:13:25.26 Archive.in :pressurize @10:13:25.33 Archive.seek :pressurized @10:13:37.82 31.5psi after pressurize @10:13:37.84 Archive.in :compress @10:13:37.92 Archive.seek :empty @10:13:51.97 Reached 33.1 of 32.9psi after pumping 3440uL (1.99 strokes) @10:13:51.99 Archive.in :default @10:13:52.06 Archive.hold @10:13:52.13 Handoff.select :waste @10:14:04.33 delay 1:00 @10:15:04.34 Handoff.select :blocked @10:15:27.02 Handoff.select :waste @10:15:27.43 1.1psi at evacEnd @10:15:27.51 Air.seek :fullVent @10:15:28.94 Archive.in :fast @10:15:29.01 Archive.seek :topVent @10:15:36.05 Seawater evacuated, Pressure above/below filter released. @10:15:36.14 Lysis1.in :slow @10:15:36.21 Lysis1.seek 1050uL @10:16:06.28 delay 5 seconds @10:16:11.30 Handoff.select :blocked @10:16:11.51 Lysis1.seek 450uL @10:17:11.53 delay 3 seconds @10:17:14.60 Air.in mix with 1.40048 parts Lysis1 @10:17:15.05 Air.seek :evac @10:17:15.10 Lysis1.seek 300uL @10:17:30.22 Lysis1.seek :empty @10:18:00.34 Lysis buffer delivered @34.8psi @10:18:00.35 Polling Can every 2:00 @10:18:00.90 PuckHeater.configure :default #ambient 11.9C @10:18:00.95 PuckHeater.seek 80.2C @10:19:14.06 PuckHeater.hold 2:00,80.2C @10:21:14.01 Polling Can every 2:00 @10:21:14.55 PuckHeater.configure :default #ambient 12.0C @10:21:14.61 PuckHeater.hold 30 seconds,79.2C @10:21:44.78 Polling Can every 2:00 @10:21:45.32 PuckHeater.configure :default #ambient 11.9C @10:21:45.38 PuckHeater.hold 30 seconds,78.6C @10:22:15.57 Polling Can every 2:00 @10:22:16.13 PuckHeater.hold 10:00,78.1C @10:32:15.53 Lysis complete. Syringe pressure @34.9psi @10:32:15.55 Archive syringe pumping up to 5 strokes to reach 40psi @10:32:15.57 Archive stroke #1 of 5 @10:32:15.67 Archive.in :pressurize @10:32:15.73 Archive.seek :pressurized @10:32:28.22 34.1psi after pressurize @10:32:33.80 33.8psi after pressurize (stable) @10:32:33.82 Archive.in :compress @10:32:33.89 Archive.seek :empty @10:32:39.66 Reached 40.5 of 40.0psi after pumping 1539uL (0.89 strokes) @10:32:39.67 Archive.in :default @10:32:39.75 Archive.hold @10:32:39.85 delay 2 seconds @10:32:41.88 Archive.in :fast @10:32:41.94 Archive.seek :full @10:32:48.44 Lysis1.seek 138uL @10:33:02.32 delay 1 second @10:33:03.35 Archive.seek :full @10:33:03.39 Lysis1.seek :empty @10:33:17.34 Handoff.select :blocked @10:33:17.45 Initial air pressure @49.2psi @10:33:17.53 Lysis1.in :extract @10:33:17.63 Lysis1.seek 600uL,nil @10:33:19.88 Archive syringe pumping up to 6 strokes to reach 25psi @10:33:19.90 Archive stroke #1 of 6 @10:33:19.99 Archive.in :pressurize @10:33:20.07 Archive.seek :pressurized @10:33:32.98 10.6psi after pressurize @10:34:20.11 14.4psi after pressurize (stable) @10:34:20.16 Archive.in :compress @10:34:20.25 Archive.seek :empty @10:34:27.46 Archive.in :default @10:34:27.52 Archive.hold @10:34:27.70 Initial 600uL lysate @16.5psi @10:34:27.71 Handoff.select :blocked @10:34:27.83 Initial air pressure @16.5psi @10:34:27.90 Lysis1.seek 825uL,nil @10:34:29.21 Archive syringe pumping up to 6 strokes to reach 17psi @10:34:29.22 Archive stroke #1 of 6 @10:34:29.31 Archive.in :compress @10:34:29.38 Archive.seek :empty @10:34:35.47 Archive.in :default @10:34:35.53 Archive.hold @10:34:35.74 Recovered 825uL lysate @16.4psi @10:34:35.77 Air.in :halfSpeed @10:34:35.84 Air.seek :fullVent @10:34:38.07 Puck Top Vented, bottom @0.4psi @10:34:38.09 Handoff.select :waste @10:34:38.22 delay 5 seconds @10:34:43.29 Handoff open to waste, bottom @-0.1psi @10:34:43.31 Air.in :default @10:34:43.39 Air.seek :evac @10:34:44.81 Lysis2.in :DAmix @10:34:44.92 Lysis1.in :DAmix @10:34:45.40 Lysis2.seek 1480uL @10:34:45.45 Lysis1.seek 575uL @10:35:20.55 delay 5 seconds @10:35:25.56 Handoff.select SPR @10:35:25.69 SPR.command "start 2 14" @10:35:26.76 SPR stabilized @10:35:26.89 delay 10 seconds @10:35:36.91 SPR.command "start 1 0" @10:35:40.12 Waiting up to 4:00 for SPR to enter state 1 @10:37:44.37 SPR.command "hold_on" @10:37:45.80 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:37:46.32 Lysis2.seek 1180uL @10:37:46.38 Lysis1.seek 275uL @10:38:28.40 delay 15 seconds @10:38:43.42 SPR.command "hold_off" @10:38:46.93 Waiting up to 38:00 for SPR to enter state 7 @10:56:46.98 SPRsummary:10.7RIU,178RIU,176RIU,236RIU,none,none,1.7ng/L after 3131s @11:03:24.20 SPR analysis completed @11:03:29.75 Ended log spr23apr1610h.spr.bz2 @11:03:29.76 SPR.command "stats_off" @11:03:31.32 -> Cmd.stop @11:03:31.41 Handoff.select :close @11:03:31.54 Gate.power :heat,:OFF @11:03:31.56 Gate.power :cartridge,:OFF @11:03:31.65 PinPort.in :unlocking @11:03:31.73 PinPort.seek :clear @11:03:32.20 Carousel.in :default @11:03:32.31 Carousel.home.seek 49 @11:03:32.61 Plunge.seek :locked @11:03:33.49 Archive.in :unlocking @11:03:33.62 Archive.seek :clear @11:03:33.71 Air.in :unlocking @11:03:33.82 Air.seek :clear @11:03:33.87 Lysis1.in :unlocking @11:03:34.03 Lysis1.seek :clear @11:03:34.07 Lysis2.in :unlocking @11:03:34.18 Lysis2.seek :clear @11:03:38.13 Gate.power :motor,:OFF @11:03:38.15 Gate.power :core,:OFF @11:03:38.17 Gate.power :analytic1,:OFF @11:03:38.20 Gate.power :analytic2,:OFF @11:03:38.22 Gate.power :analytic3,:OFF @11:03:38.24 Gate.power :main,:OFF @11:03:38.27 Safely stopped and ready to power off :STOPPED