@15:05:34.38PDT16-Apr-23 <MAIN> >BREAK< ESP3 Gateway v1.42 for core bus
@15:05:34.43 >BREAK< ESP3 Gateway v1.42 for cartridge bus
@15:05:37.01 
Configuration for honu by brent@mbari.org 4/12/23
/home/esp/esp2/type/auv/honu/configure.rb
@15:05:37.53 Sea pressure (Abs) tare is 6% of full scale
@15:05:42.12 Polling Can every 2:00
@15:05:42.62 Clients may connect on TCP port 9999
@15:05:42.65 Connecting to 10.89.5.2:9999
@15:05:42.69 Waiting for espclients...
@15:05:42.83 <LRAUV> Session Begun
@15:05:44.76 -> showStatus
true
@15:05:46.77 -> Cmd.loadCartridge -10
@15:05:46.83 Gate.power :main,:ON,limit to 5000mA
@15:05:46.86 Gate.power :analytic1,:ON,limit to 5000mA
@15:05:46.88 Gate.power :analytic2,:ON,limit to 5000mA
@15:05:46.90 Gate.power :analytic3,:ON,limit to 5000mA
@15:05:47.40 Powering core bus
@15:05:49.31 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@15:05:50.10 <LRAUV> Archive.configure :default
@15:05:50.19 Air.configure :default
@15:05:50.26 Lysis1.configure :default
@15:05:50.33 Lysis2.configure :default
@15:05:50.42 Plunge.configure :default
@15:05:50.51 SamplePump.configure :default
@15:05:50.60 Toroid.configure :default
@15:05:50.69 Carousel.configure :default
@15:05:50.79 Pressure.configure :default
@15:05:50.88 ExtraPump.configure :default
@15:05:50.95 PinPort.configure :default
@15:05:51.02 All elf controllers running firmware version 4.83
@15:05:51.04 Gate.power :motor,:ON,limit to 5000mA
@15:05:51.22 <Archive_home> Archive.home.jog 0
@15:05:51.73 <PinPort_home> PinPort.home.jog 0
@15:05:51.78 <Carousel_home> Carousel.home.jog 0
@15:05:51.80 <Plunge_home> Plunge.home.jog 0
@15:05:51.83 <Air_home> Air.home.jog 0
@15:05:51.86 <Lysis2_home> Lysis2.home.jog 0
@15:05:51.91 <Toroid_home> Toroid.home.jog 0
@15:05:51.95 <Lysis1_home> Lysis1.home.jog 0
@15:05:52.38 <Archive_home> Archive.in :homing
@15:05:52.61 Archive.jog 1394
@15:05:52.80 <Plunge_home> Plunge.in :homing
@15:05:52.83 <Toroid_home> Toroid.in :homing
@15:05:52.87 <Lysis1_home> Lysis1.in :homing
@15:05:52.91 <Air_home> Air.in :homing
@15:05:52.95 <PinPort_home> PinPort.in :homing
@15:05:53.00 <Lysis2_home> Lysis2.in :homing
@15:05:53.21 <Plunge_home> Plunge.jog 1554
@15:05:53.30 <PinPort_home> PinPort.jog -106
@15:05:53.34 <Lysis1_home> Lysis1.jog 161
@15:05:53.40 <Air_home> Air.jog 1382
@15:05:53.43 <Lysis2_home> Lysis2.jog 1405
@15:05:53.46 <Toroid_home> Toroid.jog 627
@15:05:53.70 <PinPort_home> PinPort.setRawPosition! 0
@15:05:53.74 PinPort.seek :home
@15:05:53.83 PinPort.in :default
@15:05:53.85 <Archive_home> Archive.setRawPosition! 50
@15:05:53.94 Archive.seek :home
@15:05:54.03 <Lysis1_home> Lysis1.setRawPosition! 50
@15:05:54.08 Lysis1.seek :home
@15:05:54.24 <Archive_home> Archive.in :default
@15:05:54.36 <Lysis1_home> Lysis1.in :default
@15:05:54.56 <Plunge_home> Plunge.setRawPosition! 50
@15:05:54.60 Plunge.seek :home
@15:05:54.72 <Air_home> Air.setRawPosition! 50
@15:05:54.78 Air.seek :home
@15:05:54.86 <Lysis2_home> Lysis2.setRawPosition! 50
@15:05:54.92 Lysis2.seek :home
@15:05:55.01 <Plunge_home> Plunge.in :default
@15:05:55.09 <Air_home> Air.in :default
@15:05:55.20 <Lysis2_home> Lysis2.in :default
@15:05:55.71 <Toroid_home> Toroid.setRawPosition! 0
@15:05:55.76 Toroid.seek :home
@15:05:55.83 Toroid.in :default
@15:05:55.91 <LRAUV> Toroid.seek :clear
@15:05:57.53 Carousel.in :default
@15:05:57.64 Carousel.home.seek 36
@15:05:57.89 Selecting Cartridge 48
@15:05:58.56 Carousel.seek 48
@15:06:01.30 Lysis1.in :fast
@15:06:01.37 Lysis1.seek :locked
@15:06:03.97 <Plunge> Plunge.seek :locked
@15:06:04.11 <Air> Air.in :fast
@15:06:04.17 Air.seek :locked
@15:06:05.75 <LRAUV> Carousel.reconfigure :locked
@15:06:05.89 Powering cartridge bus
@15:06:08.51 Gate.setIR 1750,5000,0,6
@15:06:08.63 Gate.setIR 0,5000,0,6
@15:06:08.70 PuckHeater.configure :default
@15:06:08.77 Gate.power :heat,:ON,limit to 5000mA
@15:06:11.45 Mounted lysis Cartridge board#Q05057
@15:06:11.49 Using default cartridge heat loss curve for HeatingAccelInCarousel
48
@15:06:24.35 -> Cmd.startFiltering
@15:06:24.60 <FILTERING> Preparing to filter cartridge 48
92.07
@15:06:24.87 Priming sample loop w/100ml, bypass w/10ml
@15:06:24.89 SamplePump.setPosition! 0ml
@15:06:24.95 Bypass.open
@15:06:26.41 Intake.open
@15:06:27.17 Sea 9.6m deep (28.8psia)
@15:06:27.19 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@15:06:29.77 Vacuum tare is 60% of full scale
@15:06:29.90 Pressure.in :default
@15:06:29.97 Pressure.hold
@15:06:30.16 SamplePump.in :prime
@15:06:30.23 SamplePump.seek 10ml,59 seconds
@15:06:39.00 Pressure.stop
@15:06:39.05 SamplePump.setPosition! 0ml
@15:06:39.10 Exhaust.open
@15:06:40.57 <closeBypass> Bypass.close
@15:06:42.05 <FILTERING> Pressure.in :default
@15:06:42.11 Pressure.hold
@15:06:42.23 Sea 9.7m deep (29.0psia)
@15:06:42.37 SamplePump.in :prime
@15:06:42.45 SamplePump.seek 100ml,5:16
@15:08:20.53 Pressure.stop
@15:08:20.60 Bypass.open
@15:08:21.27 Intake.close
@15:08:22.07 Toroid.in :hold
@15:08:22.13 Toroid.seek :crack
@15:08:23.69 Toroid.seek :cartridge
@15:08:24.79 Toroid.in :default
@15:08:24.86 Toroid.seek :clear
@15:08:26.83 Pressurizing cartridge to 26psi above 28.2psia
@15:08:26.84 SamplePump.setPosition! 0ml
@15:08:26.90 Intake.open
@15:08:27.56 Bypass.close
@15:08:28.26 Exhaust.close
@15:08:29.06 Pressure.in :default
@15:08:29.12 Pressure.hold
@15:08:29.36 SamplePump.in :charge
@15:08:29.43 SamplePump.seek 2.3ml,42 seconds
@15:08:38.50 Pressure.stop
@15:08:38.56 Intake.close
@15:08:39.30 Pressurized cartridge with 0.388ml to 26.1psi
@15:08:39.31 Isolated @53.0psia (24.7psi)
@15:08:39.33 Verifying cartridge holds at least 0.65..0.85 of 53.0psia
@15:08:39.36 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@15:08:44.02 Sampling 1000.0ml in 1:25:20
@15:08:44.04 SamplePump.setPosition! 0ml
@15:08:44.11 Exhaust.open
@15:08:44.87 Intake.open
@15:08:45.71 Pressure.in :default
@15:08:45.78 Pressure.hold
@15:08:45.90 Sea 9.7m deep (29.1psia)
@15:08:45.91 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@15:08:46.07 SamplePump.in :sample
@15:08:46.14 SamplePump.seek 1000ml,1:23:51
@15:08:48.74 <sampling> Cartridge 48 filtering 1/1000ml at 0.38ml/s with 26.3psi vacuum 7.2m deep (25.3psia)
@15:09:48.75 Cartridge 48 filtering 23/1000ml at 0.34ml/s with 26.0psi vacuum 7.7m deep (26.1psia)
@15:10:48.75 Cartridge 48 filtering 45/1000ml at 0.36ml/s with 21.0psi vacuum 9.7m deep (29.1psia)
@15:11:48.74 Cartridge 48 filtering 66/1000ml at 0.35ml/s with 26.7psi vacuum 8.1m deep (26.6psia)
@15:12:48.75 Cartridge 48 filtering 88/1000ml at 0.36ml/s with 25.5psi vacuum 9.3m deep (28.4psia)
@15:13:48.74 Cartridge 48 filtering 108/1000ml at 0.33ml/s with 22.3psi vacuum 5.8m deep (23.3psia)
@15:14:48.75 Cartridge 48 filtering 129/1000ml at 0.33ml/s with 27.1psi vacuum 7.5m deep (25.7psia)
@15:15:48.75 Cartridge 48 filtering 149/1000ml at 0.31ml/s with 24.1psi vacuum 7.7m deep (26.1psia)
@15:16:48.75 Cartridge 48 filtering 169/1000ml at 0.33ml/s with 20.4psi vacuum 10.4m deep (30.1psia)
@15:17:48.74 Cartridge 48 filtering 188/1000ml at 0.33ml/s with 25.0psi vacuum 10.5m deep (30.2psia)
@15:18:48.74 Cartridge 48 filtering 207/1000ml at 0.30ml/s with 19.6psi vacuum 9.3m deep (28.4psia)
@15:19:49.17 Cartridge 48 filtering 226/1000ml at 0.29ml/s with 23.5psi vacuum 7.8m deep (26.2psia)
@15:20:48.75 Cartridge 48 filtering 243/1000ml at 0.28ml/s with 26.0psi vacuum 9.1m deep (28.2psia)
@15:21:48.75 Cartridge 48 filtering 261/1000ml at 0.28ml/s with 26.9psi vacuum 8.7m deep (27.6psia)
@15:22:48.75 Cartridge 48 filtering 278/1000ml at 0.31ml/s with 26.5psi vacuum 7.6m deep (25.9psia)
@15:23:48.75 Cartridge 48 filtering 295/1000ml at 0.29ml/s with 25.5psi vacuum 8.8m deep (27.7psia)
@15:24:48.75 Cartridge 48 filtering 312/1000ml at 0.28ml/s with 22.6psi vacuum 7.1m deep (25.1psia)
@15:25:48.75 Cartridge 48 filtering 329/1000ml at 0.31ml/s with 23.8psi vacuum 7.5m deep (25.8psia)
@15:26:48.75 Cartridge 48 filtering 346/1000ml at 0.25ml/s with 25.0psi vacuum 7.1m deep (25.2psia)
@15:27:48.74 Cartridge 48 filtering 362/1000ml at 0.27ml/s with 24.8psi vacuum 9.2m deep (28.3psia)
@15:28:48.75 Cartridge 48 filtering 379/1000ml at 0.27ml/s with 20.6psi vacuum 9.4m deep (28.6psia)
@15:29:48.74 Cartridge 48 filtering 395/1000ml at 0.27ml/s with 23.5psi vacuum 7.3m deep (25.5psia)
@15:30:48.75 Cartridge 48 filtering 411/1000ml at 0.26ml/s with 24.9psi vacuum 7.2m deep (25.4psia)
@15:31:48.74 Cartridge 48 filtering 427/1000ml at 0.26ml/s with 25.5psi vacuum 8.8m deep (27.7psia)
@15:32:48.75 Cartridge 48 filtering 442/1000ml at 0.25ml/s with 22.3psi vacuum 7.7m deep (26.1psia)
@15:33:48.74 Cartridge 48 filtering 458/1000ml at 0.24ml/s with 24.0psi vacuum 8.2m deep (26.8psia)
@15:34:48.75 Cartridge 48 filtering 473/1000ml at 0.27ml/s with 21.4psi vacuum 9.2m deep (28.4psia)
@15:35:48.75 Cartridge 48 filtering 488/1000ml at 0.25ml/s with 23.1psi vacuum 8.9m deep (27.8psia)
@15:36:48.75 Cartridge 48 filtering 503/1000ml at 0.25ml/s with 23.9psi vacuum 8.9m deep (27.8psia)
@15:37:48.75 Cartridge 48 filtering 518/1000ml at 0.25ml/s with 24.3psi vacuum 9.0m deep (27.9psia)
@15:38:48.75 Cartridge 48 filtering 533/1000ml at 0.25ml/s with 26.7psi vacuum 7.2m deep (25.3psia)
@15:39:48.75 Cartridge 48 filtering 548/1000ml at 0.24ml/s with 25.5psi vacuum 8.9m deep (27.8psia)
@15:40:48.75 Cartridge 48 filtering 562/1000ml at 0.24ml/s with 26.0psi vacuum 8.1m deep (26.7psia)
@15:41:48.75 Cartridge 48 filtering 577/1000ml at 0.23ml/s with 23.0psi vacuum 8.8m deep (27.7psia)
@15:42:48.75 Cartridge 48 filtering 591/1000ml at 0.23ml/s with 23.4psi vacuum 8.8m deep (27.7psia)
@15:43:48.75 Cartridge 48 filtering 605/1000ml at 0.22ml/s with 22.4psi vacuum 9.2m deep (28.2psia)
@15:44:48.75 Cartridge 48 filtering 618/1000ml at 0.21ml/s with 22.4psi vacuum 9.1m deep (28.1psia)
@15:45:48.75 Cartridge 48 filtering 631/1000ml at 0.22ml/s with 23.8psi vacuum 8.0m deep (26.5psia)
@15:46:48.75 Cartridge 48 filtering 644/1000ml at 0.22ml/s with 26.6psi vacuum 7.7m deep (26.1psia)
@15:47:48.75 Cartridge 48 filtering 657/1000ml at 0.23ml/s with 23.7psi vacuum 9.2m deep (28.3psia)
@15:48:48.75 Cartridge 48 filtering 670/1000ml at 0.20ml/s with 24.6psi vacuum 7.7m deep (26.1psia)
@15:49:48.75 Cartridge 48 filtering 682/1000ml at 0.21ml/s with 22.8psi vacuum 7.4m deep (25.6psia)
@15:50:48.75 Cartridge 48 filtering 695/1000ml at 0.21ml/s with 24.5psi vacuum 7.8m deep (26.2psia)
@15:51:48.75 Cartridge 48 filtering 708/1000ml at 0.20ml/s with 25.1psi vacuum 8.7m deep (27.6psia)
@15:52:48.75 Cartridge 48 filtering 720/1000ml at 0.20ml/s with 24.1psi vacuum 8.7m deep (27.5psia)
@15:53:48.75 Cartridge 48 filtering 732/1000ml at 0.21ml/s with 21.3psi vacuum 8.7m deep (27.5psia)
@15:54:48.75 Cartridge 48 filtering 745/1000ml at 0.21ml/s with 22.0psi vacuum 9.4m deep (28.6psia)
@15:55:48.75 Cartridge 48 filtering 757/1000ml at 0.20ml/s with 24.2psi vacuum 7.4m deep (25.6psia)
@15:56:49.14 Cartridge 48 filtering 769/1000ml at 0.20ml/s with 24.1psi vacuum 7.6m deep (25.9psia)
@15:57:48.75 Cartridge 48 filtering 781/1000ml at 0.20ml/s with 26.2psi vacuum 7.7m deep (26.0psia)
@15:58:48.75 Cartridge 48 filtering 794/1000ml at 0.20ml/s with 25.5psi vacuum 8.2m deep (26.8psia)
@15:59:48.75 Cartridge 48 filtering 806/1000ml at 0.21ml/s with 22.7psi vacuum 9.1m deep (28.2psia)
@16:00:48.75 Cartridge 48 filtering 818/1000ml at 0.20ml/s with 26.6psi vacuum 7.5m deep (25.8psia)
@16:01:48.75 Cartridge 48 filtering 830/1000ml at 0.21ml/s with 25.5psi vacuum 8.8m deep (27.7psia)
@16:02:48.75 Cartridge 48 filtering 842/1000ml at 0.20ml/s with 26.2psi vacuum 8.8m deep (27.6psia)
@16:03:48.75 Cartridge 48 filtering 854/1000ml at 0.20ml/s with 24.3psi vacuum 8.6m deep (27.4psia)
@16:04:20.39 <FILTERING> Flow averaged 0.26ml/s over 55:35
@16:04:20.42 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi
@16:04:21.01 Relieved excess vacuum in .6 seconds
@16:04:21.04 SamplePump.seek 1000ml,12:07
@16:04:48.74 <sampling> Cartridge 48 filtering 866/1000ml at 0.20ml/s with 23.0psi vacuum 8.4m deep (27.1psia)
@16:05:48.75 Cartridge 48 filtering 878/1000ml at 0.20ml/s with 25.2psi vacuum 8.8m deep (27.7psia)
@16:06:48.75 Cartridge 48 filtering 891/1000ml at 0.20ml/s with 26.5psi vacuum 7.5m deep (25.8psia)
@16:07:48.75 Cartridge 48 filtering 903/1000ml at 0.20ml/s with 26.4psi vacuum 8.6m deep (27.4psia)
@16:08:10.10 <FILTERING> Flow averaged 0.20ml/s over 3:50
@16:08:10.14 Waiting up to 14 seconds for vacuum to reduce from 28.5 to 18.0psi
@16:08:10.73 Relieved excess vacuum in .6 seconds
@16:08:10.76 SamplePump.seek 1000ml,8:16
@16:08:22.54 Flow averaged 0.20ml/s over 12 seconds
@16:08:22.56 <closeIntake> Intake.close
@16:08:22.62 <closeExhaust> Exhaust.close
@16:08:22.84 <FILTERING> Toroid.in :hold
@16:08:22.91 Toroid.seek :bypass
@16:08:24.55 Toroid.in :default
@16:08:24.62 Toroid.seek :clear
@16:08:26.24 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@16:08:26.26 Pressure.stop
@16:08:26.45 Sampled  909.3ml
@16:08:28.66 <LRAUV> -> Cmd.startProcessing
120.0
@16:08:30.17 <PROCESSING> SPR.command "read_sequences"
@16:08:30.76 SPR.command "stats_on"
@16:08:30.89 SPR.command "start 0 1"
@16:08:31.61 <SPRlogger> SPR.start /var/log/esp/spr23apr1616h.spr.bz2
@16:08:34.10 <PROCESSING> Handoff.select :waste
@16:08:40.80 Syringe tare is 15% of full scale
@16:08:40.83 PinPort.in :locking
@16:08:40.92 PinPort.seek :locked
@16:08:41.41 <posArchive> Archive.in :fast
@16:08:41.45 <posLyS2> Lysis2.in :fast
@16:08:41.52 <PROCESSING> Lysis1.seek 1350uL
@16:08:41.63 <posArchive> Archive.seek :full
@16:08:41.74 <posLyS2> Lysis2.seek :full
@16:08:43.69 <PROCESSING> Plunge.seek :reagent
@16:08:44.59 Evacuating puck with filter bubble point of 50psi
@16:08:44.61 PinPort.seek :locked
@16:08:44.75 Handoff.select :waste
@16:08:51.34 Air syringe pumping up to 1 strokes to reach 8psi
@16:08:51.35 Air stroke #1 of 1
@16:08:51.44 Air.in :pressurize
@16:08:51.51 Air.seek :pressurized
@16:08:57.24 0.6psi after pressurize
@16:08:57.26 Air.in :compress
@16:08:57.33 Air.seek :evac
@16:09:00.34 0.9psi after compress
@16:09:10.91 0.1psi after compress (stable)
@16:09:10.94 Air.in :default
@16:09:11.04 Air.hold
@16:09:11.24 at 0.0psi Opened to waste
@16:09:11.25 Handoff.select :blocked
@16:09:11.39 Archive syringe pumping up to 8 strokes to reach 50psi
@16:09:11.40 Archive stroke #1 of 8
@16:09:11.48 Archive.in :pressurize
@16:09:11.56 Archive.seek :pressurized
@16:09:24.06 6.2psi after pressurize
@16:09:29.63 6.2psi after pressurize (stable)
@16:09:29.65 Archive.in :compress
@16:09:29.74 Archive.seek :empty
@16:09:44.36 6.5psi after compress
@16:09:54.93 7.9psi after compress (stable)
@16:09:54.95 Archive.in :gulp
@16:09:55.04 Archive.seek :topVent
@16:10:01.28 7.8psi after gulp
@16:10:06.85 7.8psi after gulp (stable)
@16:10:06.86 Archive stroke #2 of 8
@16:10:06.96 Archive.in :pressurize
@16:10:07.03 Archive.seek :pressurized
@16:10:19.51 8.0psi after pressurize
@16:10:25.09 8.0psi after pressurize (stable)
@16:10:25.10 Archive.in :compress
@16:10:25.54 Archive.seek :empty
@16:10:40.16 8.7psi after compress
@16:10:50.73 9.4psi after compress (stable)
@16:10:50.75 Archive.in :gulp
@16:10:50.83 Archive.seek :topVent
@16:10:57.07 10.3psi after gulp
@16:11:02.64 10.9psi after gulp (stable)
@16:11:02.65 Archive stroke #3 of 8
@16:11:02.75 Archive.in :pressurize
@16:11:02.82 Archive.seek :pressurized
@16:11:15.31 12.4psi after pressurize
@16:11:20.89 12.9psi after pressurize (stable)
@16:11:20.91 Archive.in :compress
@16:11:20.99 Archive.seek :empty
@16:11:35.60 17.2psi after compress
@16:12:04.69 41.9psi after compress (stable)
@16:12:04.71 Archive.in :gulp
@16:12:04.79 Archive.seek :topVent
@16:12:11.03 43.0psi after gulp
@16:12:16.60 43.1psi after gulp (stable)
@16:12:16.61 Archive stroke #4 of 8
@16:12:16.71 Archive.in :pressurize
@16:12:16.78 Archive.seek :pressurized
@16:12:29.27 42.7psi after pressurize
@16:12:34.84 42.8psi after pressurize (stable)
@16:12:34.85 Archive.in :compress
@16:12:34.94 Archive.seek :empty
@16:12:49.55 43.0psi after compress
@16:13:05.01 Reached 50.2 of 50.0psi after pumping 6920uL (4.00 strokes)
@16:13:05.02 Archive.in :default
@16:13:05.10 Archive.hold
@16:13:05.17 Handoff.select :waste
@16:13:19.88 delay 1:00
@16:14:19.89 Handoff.select :blocked
@16:14:42.58 Handoff.select :waste
@16:14:42.70 0.6psi at evacEnd
@16:14:42.71 Handoff.select :blocked
@16:14:42.85 Archive.in :fast
@16:14:42.93 Archive.seek :topVent
@16:14:56.61 Stable @0.3psi after Handoff blocked
@16:14:56.63 Archive syringe pumping up to 8 strokes to reach 30psi
@16:14:56.65 Archive stroke #1 of 8
@16:14:56.75 Archive.in :pressurize
@16:14:56.82 Archive.seek :pressurized
@16:15:09.31 0.4psi after pressurize
@16:15:09.33 Archive.in :compress
@16:15:09.41 Archive.seek :empty
@16:15:24.10 0.7psi after compress
@16:15:40.73 2.9psi after compress (stable)
@16:15:40.76 Archive.in :gulp
@16:15:40.84 Archive.seek :topVent
@16:15:47.08 3.9psi after gulp
@16:15:52.65 4.4psi after gulp (stable)
@16:15:52.66 Archive stroke #2 of 8
@16:15:52.76 Archive.in :pressurize
@16:15:52.83 Archive.seek :pressurized
@16:16:05.32 5.4psi after pressurize
@16:16:05.34 Archive.in :compress
@16:16:05.42 Archive.seek :empty
@16:16:20.04 8.8psi after compress
@16:16:52.96 21.0psi after compress (stable)
@16:16:52.99 Archive.in :gulp
@16:16:53.07 Archive.seek :topVent
@16:16:59.31 22.0psi after gulp
@16:17:04.89 22.4psi after gulp (stable)
@16:17:04.90 Archive stroke #3 of 8
@16:17:05.01 Archive.in :pressurize
@16:17:05.08 Archive.seek :pressurized
@16:17:17.56 23.2psi after pressurize
@16:17:17.58 Archive.in :compress
@16:17:17.66 Archive.seek :empty
@16:17:32.31 25.3psi after compress
@16:17:40.15 Reached 30.4 of 30.3psi after pumping 5190uL (3.00 strokes)
@16:17:40.16 Archive.in :default
@16:17:40.24 Archive.hold
@16:17:40.32 Handoff.select :waste
@16:17:47.52 delay 1:00
@16:18:47.53 Handoff.select :blocked
@16:19:10.21 Handoff.select :waste
@16:19:10.33 1.7psi at evacEnd
@16:19:10.35 Air.seek :fullVent
@16:19:11.78 Archive.in :fast
@16:19:11.86 Archive.seek :topVent
@16:19:18.96 Seawater evacuated, Pressure above/below filter released.
@16:19:19.40 Lysis1.in :slow
@16:19:19.55 Lysis1.seek 1050uL
@16:19:49.59 delay 5 seconds
@16:19:54.60 Handoff.select :blocked
@16:19:54.78 Lysis1.seek 450uL
@16:20:54.77 delay 3 seconds
@16:20:57.85 Air.in mix with 1.40048 parts Lysis1
@16:20:58.59 <Airmixer> Air.seek :evac
@16:20:58.63 <PROCESSING> Lysis1.seek 300uL
@16:21:13.77 Lysis1.seek :empty
@16:21:43.92 Lysis buffer delivered @32.6psi
@16:21:43.93 Polling Can every 2:00
@16:21:44.48 PuckHeater.configure :default #ambient 10.9C
@16:21:44.54 PuckHeater.seek 80.3C
@16:23:06.03 PuckHeater.hold 2:00,80.3C
@16:25:06.43 Polling Can every 2:00
@16:25:06.97 PuckHeater.configure :default #ambient 11.0C
@16:25:07.03 PuckHeater.hold 30 seconds,79.2C
@16:25:37.36 Polling Can every 2:00
@16:25:37.90 PuckHeater.configure :default #ambient 10.9C
@16:25:37.96 PuckHeater.hold 30 seconds,78.7C
@16:26:08.21 Polling Can every 2:00
@16:26:08.76 PuckHeater.hold 10:00,78.2C
@16:36:10.05 Lysis complete. Syringe pressure @42.1psi
@16:36:10.08 Archive syringe pumping up to 5 strokes to reach 40psi
@16:36:10.09 Archive stroke #1 of 5
@16:36:10.19 Archive.in :pressurize
@16:36:10.30 Archive.seek :pressurized
@16:36:10.47 Reached 42.1 of 40.0psi after pumping 0uL (0.00 strokes)
@16:36:10.49 Archive.in :default
@16:36:10.56 Archive.hold
@16:36:10.63 delay 2 seconds
@16:36:12.65 Archive.in :fast
@16:36:12.72 Archive.seek :full
@16:36:12.92 Lysis1.seek 138uL
@16:36:26.80 delay 1 second
@16:36:27.82 <fillArchive> Archive.seek :full
@16:36:27.86 <PROCESSING> Lysis1.seek :empty
@16:36:41.80 Handoff.select :blocked
@16:36:41.90 Initial air pressure @43.0psi
@16:36:41.98 Lysis1.in :extract
@16:36:42.07 Lysis1.seek 600uL,nil
@16:36:43.53 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@16:36:43.56 Archive stroke #1 of 6
@16:36:43.67 Archive.in :pressurize
@16:36:43.74 Archive.seek :pressurized
@16:36:56.26 15.2psi after pressurize
@16:37:01.86 15.4psi after pressurize (stable)
@16:37:01.88 Archive.in :compress
@16:37:01.96 Archive.seek :empty
@16:37:15.29 Archive.in :default
@16:37:15.36 Archive.hold
@16:37:15.52 <PROCESSING> Initial 600uL lysate @15.8psi
@16:37:15.53 Handoff.select :blocked
@16:37:15.64 Initial air pressure @17.8psi
@16:37:15.72 Lysis1.seek 825uL,nil
@16:37:17.22 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@16:37:17.24 Archive stroke #1 of 6
@16:37:17.41 Archive.in :compress
@16:37:17.49 Archive.seek :empty
@16:37:18.16 Reached 17.1 of 17.0psi after pumping 1715uL (0.99 strokes)
@16:37:18.17 Archive.in :default
@16:37:18.24 Archive.hold
@16:37:19.04 Archive syringe pumping up to 6 strokes to reach 17psi
@16:37:19.05 Archive stroke #1 of 6
@16:37:19.14 Archive.in :compress
@16:37:19.23 Archive.seek :empty
@16:37:20.10 14.8psi after compress
@16:37:20.58 Reached 17.5 of 17.0psi after pumping 1730uL (1.00 strokes)
@16:37:20.60 Archive.in :default
@16:37:20.68 Archive.hold
@16:37:21.12 <PROCESSING> Recovered 825uL lysate @16.2psi
@16:37:21.16 Air.in :halfSpeed
@16:37:21.24 Air.seek :fullVent
@16:37:23.45 Puck Top Vented, bottom @18.6psi
@16:37:23.46 Handoff.select :waste
@16:37:23.60 delay 5 seconds
@16:37:28.66 Handoff open to waste, bottom @0.2psi
@16:37:28.68 Air.in :default
@16:37:28.78 Air.seek :evac
@16:37:30.20 Lysis2.in :DAmix
@16:37:30.32 Lysis1.in :DAmix
@16:37:30.80 <Lysis2mixer> Lysis2.seek 1480uL
@16:37:30.84 <PROCESSING> Lysis1.seek 575uL
@16:38:05.95 delay 5 seconds
@16:38:10.96 Handoff.select SPR
@16:38:11.09 SPR.command "start 2 14"
@16:38:11.78 SPR stabilized
@16:38:11.92 delay 10 seconds
@16:38:21.93 SPR.command "start 1 0"
@16:38:25.14 Waiting up to 4:00 for SPR to enter state 1
@16:40:29.40 SPR.command "hold_on"
@16:40:31.30 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@16:40:31.75 <Lysis2mixer> Lysis2.seek 1180uL
@16:40:31.78 <PROCESSING> Lysis1.seek 275uL
@16:41:13.83 delay 15 seconds
@16:41:28.86 SPR.command "hold_off"
@16:41:31.46 Waiting up to 38:00 for SPR to enter state 7
@16:59:30.11 <SPRlogger> SPRsummary:15.8RIU,177RIU,175RIU,237RIU,none,none,2.16ng/L after 3231s
@17:06:07.33 <PROCESSING> SPR analysis completed
@17:06:13.41 <SPRlogger> Ended log spr23apr1616h.spr.bz2
@17:06:13.42 <PROCESSING> SPR.command "stats_off"
@17:06:14.70 <LRAUV> -> Cmd.stop
@17:06:14.80 <closeHandoff> Handoff.select :close
@17:06:14.93 <LRAUV> Gate.power :heat,:OFF
@17:06:14.95 Gate.power :cartridge,:OFF
@17:06:15.03 PinPort.in :unlocking
@17:06:15.10 PinPort.seek :clear
@17:06:15.58 Carousel.in :default
@17:06:15.70 Carousel.home.seek 48
@17:06:15.99 Plunge.seek :locked
@17:06:16.86 <Archive> Archive.in :unlocking
@17:06:17.00 <Air> Air.in :unlocking
@17:06:17.03 <Archive> Archive.seek :clear
@17:06:17.20 <Air> Air.seek :clear
@17:06:17.24 <Lysis1> Lysis1.in :unlocking
@17:06:17.42 <Lysis2> Lysis2.in :unlocking
@17:06:17.45 <Lysis1> Lysis1.seek :clear
@17:06:17.56 <Lysis2> Lysis2.seek :clear
@17:06:21.63 <LRAUV> Gate.power :motor,:OFF
@17:06:21.64 Gate.power :core,:OFF
@17:06:21.67 Gate.power :analytic1,:OFF
@17:06:21.70 Gate.power :analytic2,:OFF
@17:06:21.72 Gate.power :analytic3,:OFF
@17:06:21.75 Gate.power :main,:OFF
@17:06:21.77 Safely stopped and ready to power off
:STOPPED