@00:32:32.24PDT19-Apr-23 <MAIN> >BREAK< ESP3 Gateway v1.42 for core bus
@00:32:32.29 >BREAK< ESP3 Gateway v1.42 for cartridge bus
@00:32:35.00 
Configuration for honu by brent@mbari.org 4/12/23
/home/esp/esp2/type/auv/honu/configure.rb
@00:32:35.46 Sea pressure (Abs) tare is 6% of full scale
@00:32:40.71 Polling Can every 2:00
@00:32:41.18 Clients may connect on TCP port 9999
@00:32:41.19 Connecting to 10.89.5.2:9999
@00:32:41.22 Waiting for espclients...
@00:32:41.58 <LRAUV> Session Begun
@00:32:43.51 -> showStatus
true
@00:32:45.54 -> Cmd.loadCartridge -10
@00:32:45.60 Gate.power :main,:ON,limit to 5000mA
@00:32:45.62 Gate.power :analytic1,:ON,limit to 5000mA
@00:32:45.65 Gate.power :analytic2,:ON,limit to 5000mA
@00:32:45.67 Gate.power :analytic3,:ON,limit to 5000mA
@00:32:45.80 Powering core bus
@00:32:48.07 <Probe-AMspr> SPR Module 1, FW Rev. B, FPGA Rev. C
@00:32:48.51 <LRAUV> Archive.configure :default
@00:32:48.60 Air.configure :default
@00:32:48.67 Lysis1.configure :default
@00:32:48.74 Lysis2.configure :default
@00:32:48.83 Plunge.configure :default
@00:32:48.92 SamplePump.configure :default
@00:32:49.01 Toroid.configure :default
@00:32:49.10 Carousel.configure :default
@00:32:49.19 Pressure.configure :default
@00:32:49.28 ExtraPump.configure :default
@00:32:49.35 PinPort.configure :default
@00:32:49.42 All elf controllers running firmware version 4.83
@00:32:49.45 Gate.power :motor,:ON,limit to 5000mA
@00:32:49.68 <Archive_home> Archive.home.jog 0
@00:32:50.13 <Carousel_home> Carousel.home.jog 0
@00:32:50.18 <Toroid_home> Toroid.home.jog 0
@00:32:50.21 <Lysis1_home> Lysis1.home.jog 0
@00:32:50.25 <Lysis2_home> Lysis2.home.jog 0
@00:32:50.30 <PinPort_home> PinPort.home.jog 0
@00:32:50.32 <Plunge_home> Plunge.home.jog 0
@00:32:50.35 <Air_home> Air.home.jog 0
@00:32:50.98 <Archive_home> Archive.in :homing
@00:32:51.20 Archive.jog 1393
@00:32:51.24 <Toroid_home> Toroid.in :homing
@00:32:51.26 <Plunge_home> Plunge.in :homing
@00:32:51.30 <Lysis2_home> Lysis2.in :homing
@00:32:51.34 <Lysis1_home> Lysis1.in :homing
@00:32:51.38 <PinPort_home> PinPort.in :homing
@00:32:51.43 <Air_home> Air.in :homing
@00:32:51.66 <Toroid_home> Toroid.jog 624
@00:32:51.69 <Plunge_home> Plunge.jog 1550
@00:32:51.72 <Lysis2_home> Lysis2.jog 1407
@00:32:51.75 <Lysis1_home> Lysis1.jog 161
@00:32:51.78 <PinPort_home> PinPort.jog -105
@00:32:51.83 <Air_home> Air.jog 1372
@00:32:52.19 <PinPort_home> PinPort.setRawPosition! 0
@00:32:52.24 PinPort.seek :home
@00:32:52.33 PinPort.in :default
@00:32:52.40 <Lysis1_home> Lysis1.setRawPosition! 50
@00:32:52.46 Lysis1.seek :home
@00:32:52.61 <Archive_home> Archive.setRawPosition! 50
@00:32:52.67 Archive.seek :home
@00:32:52.75 <Lysis1_home> Lysis1.in :default
@00:32:52.95 <Archive_home> Archive.in :default
@00:32:53.07 <Lysis2_home> Lysis2.setRawPosition! 50
@00:32:53.13 Lysis2.seek :home
@00:32:53.23 <Plunge_home> Plunge.setRawPosition! 50
@00:32:53.25 <Air_home> Air.setRawPosition! 50
@00:32:53.32 <Plunge_home> Plunge.seek :home
@00:32:53.36 <Air_home> Air.seek :home
@00:32:53.50 <Lysis2_home> Lysis2.in :default
@00:32:53.65 <Plunge_home> Plunge.in :default
@00:32:53.74 <Air_home> Air.in :default
@00:32:53.93 <Toroid_home> Toroid.setRawPosition! 0
@00:32:53.98 Toroid.seek :home
@00:32:54.37 Toroid.in :default
@00:32:54.50 <LRAUV> Toroid.seek :clear
@00:32:56.12 Carousel.in :default
@00:32:56.25 Carousel.home.seek 24
@00:32:56.51 Selecting Cartridge 37
@00:32:56.78 Carousel.seek 37
@00:32:59.72 Lysis1.in :fast
@00:32:59.79 Lysis1.seek :locked
@00:33:02.39 <Plunge> Plunge.seek :locked
@00:33:02.53 <Air> Air.in :fast
@00:33:02.60 Air.seek :locked
@00:33:04.17 <LRAUV> Carousel.reconfigure :locked
@00:33:04.32 Powering cartridge bus
@00:33:06.94 Gate.setIR 1750,5000,0,6
@00:33:07.06 Gate.setIR 0,5000,0,6
@00:33:07.13 PuckHeater.configure :default
@00:33:07.19 Gate.power :heat,:ON,limit to 5000mA
@00:33:07.89 Mounted lysis Cartridge board#Q05064
@00:33:07.93 Using default cartridge heat loss curve for HeatingAccelInCarousel
37
@00:33:23.11 -> Cmd.startFiltering
@00:33:23.36 <FILTERING> Preparing to filter cartridge 37
92.07
@00:33:23.64 Priming sample loop w/100ml, bypass w/10ml
@00:33:23.65 SamplePump.setPosition! 0ml
@00:33:23.71 Bypass.open
@00:33:25.15 Intake.open
@00:33:25.90 Sea 12.6m deep (33.3psia)
@00:33:25.92 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi
@00:33:28.51 Vacuum tare is 60% of full scale
@00:33:28.64 Pressure.in :default
@00:33:28.70 Pressure.hold
@00:33:28.89 SamplePump.in :prime
@00:33:28.96 SamplePump.seek 10ml,59 seconds
@00:33:37.71 Pressure.stop
@00:33:37.76 SamplePump.setPosition! 0ml
@00:33:37.81 Exhaust.open
@00:33:39.29 <closeBypass> Bypass.close
@00:33:40.78 <FILTERING> Pressure.in :default
@00:33:40.85 Pressure.hold
@00:33:40.97 Sea 12.5m deep (33.2psia)
@00:33:41.12 SamplePump.in :prime
@00:33:41.20 SamplePump.seek 100ml,5:16
@00:35:19.03 Pressure.stop
@00:35:19.11 Bypass.open
@00:35:19.83 Intake.close
@00:35:20.60 Toroid.in :hold
@00:35:20.67 Toroid.seek :crack
@00:35:22.20 Toroid.seek :cartridge
@00:35:23.30 Toroid.in :default
@00:35:23.37 Toroid.seek :clear
@00:35:25.33 Pressurizing cartridge to 26psi above 33.6psia
@00:35:25.34 SamplePump.setPosition! 0ml
@00:35:25.40 Intake.open
@00:35:26.06 Bypass.close
@00:35:26.76 Exhaust.close
@00:35:27.60 Pressure.in :default
@00:35:27.67 Pressure.hold
@00:35:27.86 SamplePump.in :charge
@00:35:27.93 SamplePump.seek 2.3ml,42 seconds
@00:35:36.90 Pressure.stop
@00:35:36.96 Intake.close
@00:35:37.73 Pressurized cartridge with 0.379ml to 26.2psi
@00:35:37.74 Isolated @59.4psia (25.7psi)
@00:35:37.76 Verifying cartridge holds at least 0.65..0.85 of 59.4psia
@00:35:37.80 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi
@00:35:42.38 Cartridge pressure fell 4% to 57.2psia
@00:35:42.48 Sampling 1000.0ml in 1:25:20
@00:35:42.49 SamplePump.setPosition! 0ml
@00:35:42.55 Exhaust.open
@00:35:43.31 Intake.open
@00:35:44.14 Pressure.in :default
@00:35:44.20 Pressure.hold
@00:35:44.32 Sea 13.4m deep (34.4psia)
@00:35:44.33 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s
@00:35:44.49 SamplePump.in :sample
@00:35:44.56 SamplePump.seek 1000ml,1:23:51
@00:35:47.17 <sampling> Cartridge 37 filtering 1/1000ml at 0.39ml/s with 27.5psi vacuum 10.7m deep (30.4psia)
@00:36:47.20 Cartridge 37 filtering 24/1000ml at 0.38ml/s with 26.5psi vacuum 12.1m deep (32.6psia)
@00:37:47.18 Cartridge 37 filtering 48/1000ml at 0.37ml/s with 22.3psi vacuum 12.3m deep (32.9psia)
@00:38:47.18 Cartridge 37 filtering 70/1000ml at 0.37ml/s with 23.5psi vacuum 11.4m deep (31.5psia)
@00:39:47.23 Cartridge 37 filtering 92/1000ml at 0.35ml/s with 23.9psi vacuum 12.7m deep (33.5psia)
@00:40:47.18 Cartridge 37 filtering 114/1000ml at 0.33ml/s with 23.1psi vacuum 13.3m deep (34.4psia)
@00:41:47.18 Cartridge 37 filtering 135/1000ml at 0.38ml/s with 25.4psi vacuum 11.8m deep (32.2psia)
@00:42:47.55 Cartridge 37 filtering 156/1000ml at 0.34ml/s with 25.0psi vacuum 10.5m deep (30.3psia)
@00:43:47.18 Cartridge 37 filtering 176/1000ml at 0.34ml/s with 25.8psi vacuum 12.0m deep (32.4psia)
@00:44:47.19 Cartridge 37 filtering 196/1000ml at 0.36ml/s with 25.2psi vacuum 11.9m deep (32.2psia)
@00:45:47.18 Cartridge 37 filtering 216/1000ml at 0.31ml/s with 21.7psi vacuum 12.8m deep (33.6psia)
@00:46:47.18 Cartridge 37 filtering 235/1000ml at 0.33ml/s with 25.7psi vacuum 11.2m deep (31.2psia)
@00:47:47.18 Cartridge 37 filtering 254/1000ml at 0.30ml/s with 26.1psi vacuum 11.4m deep (31.5psia)
@00:48:47.18 Cartridge 37 filtering 273/1000ml at 0.30ml/s with 22.5psi vacuum 11.6m deep (31.9psia)
@00:49:47.17 Cartridge 37 filtering 291/1000ml at 0.32ml/s with 23.6psi vacuum 12.6m deep (33.4psia)
@00:50:47.18 Cartridge 37 filtering 309/1000ml at 0.27ml/s with 24.9psi vacuum 10.6m deep (30.3psia)
@00:51:47.18 Cartridge 37 filtering 327/1000ml at 0.30ml/s with 23.9psi vacuum 12.5m deep (33.2psia)
@00:52:47.17 Cartridge 37 filtering 344/1000ml at 0.31ml/s with 24.0psi vacuum 11.9m deep (32.3psia)
@00:53:47.18 Cartridge 37 filtering 361/1000ml at 0.31ml/s with 23.6psi vacuum 13.9m deep (35.2psia)
@00:54:47.18 Cartridge 37 filtering 378/1000ml at 0.27ml/s with 23.9psi vacuum 11.6m deep (31.8psia)
@00:55:47.18 Cartridge 37 filtering 395/1000ml at 0.30ml/s with 26.3psi vacuum 12.4m deep (33.0psia)
@00:56:47.18 Cartridge 37 filtering 411/1000ml at 0.28ml/s with 25.2psi vacuum 10.5m deep (30.2psia)
@00:57:47.18 Cartridge 37 filtering 428/1000ml at 0.26ml/s with 25.5psi vacuum 11.1m deep (31.1psia)
@00:58:47.18 Cartridge 37 filtering 444/1000ml at 0.27ml/s with 26.2psi vacuum 11.3m deep (31.3psia)
@00:59:47.18 Cartridge 37 filtering 460/1000ml at 0.26ml/s with 26.4psi vacuum 10.1m deep (29.6psia)
@01:00:47.17 Cartridge 37 filtering 475/1000ml at 0.24ml/s with 23.1psi vacuum 11.8m deep (32.1psia)
@01:01:47.18 Cartridge 37 filtering 491/1000ml at 0.25ml/s with 23.7psi vacuum 12.8m deep (33.6psia)
@01:02:47.18 Cartridge 37 filtering 506/1000ml at 0.26ml/s with 23.1psi vacuum 11.7m deep (31.9psia)
@01:03:47.17 Cartridge 37 filtering 521/1000ml at 0.24ml/s with 22.6psi vacuum 10.5m deep (30.2psia)
@01:04:47.17 Cartridge 37 filtering 536/1000ml at 0.27ml/s with 23.5psi vacuum 11.6m deep (31.8psia)
@01:05:47.17 Cartridge 37 filtering 551/1000ml at 0.25ml/s with 23.8psi vacuum 12.6m deep (33.3psia)
@01:06:47.18 Cartridge 37 filtering 566/1000ml at 0.23ml/s with 26.1psi vacuum 10.2m deep (29.7psia)
@01:07:47.18 Cartridge 37 filtering 580/1000ml at 0.25ml/s with 26.4psi vacuum 11.1m deep (31.2psia)
@01:08:47.18 Cartridge 37 filtering 595/1000ml at 0.23ml/s with 23.5psi vacuum 11.0m deep (31.0psia)
@01:09:47.18 Cartridge 37 filtering 609/1000ml at 0.22ml/s with 22.6psi vacuum 12.4m deep (33.0psia)
@01:10:47.17 Cartridge 37 filtering 623/1000ml at 0.25ml/s with 24.8psi vacuum 12.3m deep (32.9psia)
@01:11:47.18 Cartridge 37 filtering 637/1000ml at 0.22ml/s with 23.7psi vacuum 11.0m deep (30.9psia)
@01:12:47.17 Cartridge 37 filtering 651/1000ml at 0.25ml/s with 24.6psi vacuum 11.9m deep (32.3psia)
@01:13:47.17 Cartridge 37 filtering 665/1000ml at 0.22ml/s with 24.3psi vacuum 11.5m deep (31.7psia)
@01:14:47.17 Cartridge 37 filtering 678/1000ml at 0.24ml/s with 26.1psi vacuum 12.1m deep (32.6psia)
@01:15:47.18 Cartridge 37 filtering 691/1000ml at 0.21ml/s with 23.1psi vacuum 11.2m deep (31.3psia)
@01:16:47.18 Cartridge 37 filtering 705/1000ml at 0.22ml/s with 24.6psi vacuum 11.2m deep (31.2psia)
@01:17:47.17 Cartridge 37 filtering 718/1000ml at 0.20ml/s with 24.4psi vacuum 10.8m deep (30.6psia)
@01:18:47.17 Cartridge 37 filtering 731/1000ml at 0.23ml/s with 24.0psi vacuum 11.3m deep (31.4psia)
@01:19:47.56 Cartridge 37 filtering 744/1000ml at 0.20ml/s with 25.2psi vacuum 11.0m deep (30.9psia)
@01:20:47.18 Cartridge 37 filtering 757/1000ml at 0.23ml/s with 23.9psi vacuum 11.5m deep (31.6psia)
@01:21:47.18 Cartridge 37 filtering 770/1000ml at 0.20ml/s with 25.3psi vacuum 10.1m deep (29.6psia)
@01:22:47.18 Cartridge 37 filtering 782/1000ml at 0.20ml/s with 23.5psi vacuum 11.2m deep (31.2psia)
@01:23:47.17 Cartridge 37 filtering 795/1000ml at 0.20ml/s with 25.1psi vacuum 10.4m deep (30.1psia)
@01:24:47.18 Cartridge 37 filtering 807/1000ml at 0.22ml/s with 23.2psi vacuum 13.3m deep (34.4psia)
@01:25:47.18 Cartridge 37 filtering 820/1000ml at 0.20ml/s with 24.5psi vacuum 12.2m deep (32.8psia)
@01:26:47.18 Cartridge 37 filtering 832/1000ml at 0.20ml/s with 26.1psi vacuum 11.4m deep (31.5psia)
@01:27:47.18 Cartridge 37 filtering 844/1000ml at 0.20ml/s with 26.7psi vacuum 11.2m deep (31.3psia)
@01:28:47.18 Cartridge 37 filtering 857/1000ml at 0.20ml/s with 25.8psi vacuum 11.9m deep (32.3psia)
@01:29:47.18 Cartridge 37 filtering 869/1000ml at 0.20ml/s with 26.6psi vacuum 10.4m deep (30.1psia)
@01:30:47.17 Cartridge 37 filtering 881/1000ml at 0.20ml/s with 23.2psi vacuum 11.1m deep (31.1psia)
@01:31:47.23 Cartridge 37 filtering 893/1000ml at 0.20ml/s with 25.4psi vacuum 11.4m deep (31.5psia)
@01:32:47.17 Cartridge 37 filtering 906/1000ml at 0.20ml/s with 24.0psi vacuum 12.4m deep (33.1psia)
@01:33:36.09 <FILTERING> Flow averaged 0.26ml/s over 57:52
@01:33:36.13 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@01:33:36.72 Relieved excess vacuum in .6 seconds
@01:33:36.74 SamplePump.seek 1000ml,7:33
@01:33:47.17 <sampling> Cartridge 37 filtering 917/1000ml at 0.21ml/s with 24.2psi vacuum 10.9m deep (30.7psia)
@01:34:47.17 Cartridge 37 filtering 930/1000ml at 0.20ml/s with 27.2psi vacuum 10.3m deep (29.9psia)
@01:35:47.18 Cartridge 37 filtering 942/1000ml at 0.20ml/s with 26.0psi vacuum 11.7m deep (32.1psia)
@01:36:44.14 <FILTERING> Flow averaged 0.20ml/s over 3:08
@01:36:44.17 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi
@01:36:44.76 Relieved excess vacuum in .6 seconds
@01:36:44.79 SamplePump.seek 1000ml,4:24
@01:36:47.17 <sampling> Cartridge 37 filtering 954/1000ml at 0.21ml/s with 24.1psi vacuum 11.9m deep (32.3psia)
@01:36:48.23 <FILTERING> Flow estimate reduced to 0.16ml/s
@01:36:48.25 <closeIntake> Intake.close
@01:36:48.29 <closeExhaust> Exhaust.close
@01:36:48.52 <FILTERING> Toroid.in :hold
@01:36:48.59 Toroid.seek :bypass
@01:36:50.24 Toroid.in :default
@01:36:50.31 Toroid.seek :clear
@01:36:51.93 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged
@01:36:51.95 Pressure.stop
@01:36:52.14 Sampled  953.9ml
@01:36:54.15 <LRAUV> -> Cmd.startProcessing
120.0
@01:36:55.59 <PROCESSING> SPR.command "read_sequences"
@01:36:55.64 SPR.command "stats_on"
@01:36:55.76 SPR.command "start 0 1"
@01:36:56.48 <SPRlogger> SPR.start /var/log/esp/spr23apr1901h.spr.bz2
@01:36:59.35 <PROCESSING> Handoff.select :waste
@01:37:06.04 Syringe tare is 16% of full scale
@01:37:06.06 PinPort.in :locking
@01:37:06.15 PinPort.seek :locked
@01:37:06.63 <posArchive> Archive.in :fast
@01:37:06.68 <posLyS2> Lysis2.in :fast
@01:37:06.76 <PROCESSING> Lysis1.seek 1350uL
@01:37:06.85 <posArchive> Archive.seek :full
@01:37:06.92 <posLyS2> Lysis2.seek :full
@01:37:08.90 <PROCESSING> Plunge.seek :reagent
@01:37:09.79 Evacuating puck with filter bubble point of 50psi
@01:37:09.81 PinPort.seek :locked
@01:37:09.90 Handoff.select :waste
@01:37:16.49 Air syringe pumping up to 1 strokes to reach 8psi
@01:37:16.50 Air stroke #1 of 1
@01:37:16.60 Air.in :pressurize
@01:37:16.67 Air.seek :pressurized
@01:37:22.40 2.2psi after pressurize
@01:37:22.42 Air.in :compress
@01:37:22.50 Air.seek :evac
@01:37:25.52 1.0psi after compress
@01:37:36.10 -0.2psi after compress (stable)
@01:37:36.11 Air.in :default
@01:37:36.19 Air.hold
@01:37:36.37 at -0.2psi Opened to waste
@01:37:36.38 Handoff.select :blocked
@01:37:36.52 Archive syringe pumping up to 8 strokes to reach 50psi
@01:37:36.53 Archive stroke #1 of 8
@01:37:36.62 Archive.in :pressurize
@01:37:36.69 Archive.seek :pressurized
@01:37:49.23 0.1psi after pressurize
@01:37:54.81 0.1psi after pressurize (stable)
@01:37:54.83 Archive.in :compress
@01:37:54.91 Archive.seek :empty
@01:38:09.54 9.1psi after compress
@01:38:20.11 9.6psi after compress (stable)
@01:38:20.13 Archive.in :gulp
@01:38:20.23 Archive.seek :topVent
@01:38:26.50 10.6psi after gulp
@01:38:32.08 10.9psi after gulp (stable)
@01:38:32.09 Archive stroke #2 of 8
@01:38:32.24 Archive.in :pressurize
@01:38:32.31 Archive.seek :pressurized
@01:38:44.81 11.5psi after pressurize
@01:38:50.41 11.7psi after pressurize (stable)
@01:38:50.43 Archive.in :compress
@01:38:50.54 Archive.seek :empty
@01:39:05.18 24.9psi after compress
@01:39:41.75 45.8psi after compress (stable)
@01:39:41.77 Archive.in :gulp
@01:39:41.85 Archive.seek :topVent
@01:39:48.10 47.0psi after gulp
@01:39:53.68 47.3psi after gulp (stable)
@01:39:53.69 Archive stroke #3 of 8
@01:39:53.79 Archive.in :pressurize
@01:39:53.87 Archive.seek :pressurized
@01:40:06.40 47.4psi after pressurize
@01:40:11.97 47.6psi after pressurize (stable)
@01:40:11.99 Archive.in :compress
@01:40:12.07 Archive.seek :empty
@01:40:24.35 Reached 50.1 of 50.0psi after pumping 5139uL (2.97 strokes)
@01:40:24.36 Archive.in :default
@01:40:24.44 Archive.hold
@01:40:24.51 Handoff.select :waste
@01:40:37.70 delay 1:00
@01:41:37.71 Handoff.select :blocked
@01:42:21.90 Handoff.select :waste
@01:42:22.03 7.0psi at evacEnd
@01:42:22.04 Handoff.select :blocked
@01:42:22.18 Archive.in :fast
@01:42:22.25 Archive.seek :topVent
@01:42:43.74 Stable @3.6psi after Handoff blocked
@01:42:43.76 Archive syringe pumping up to 8 strokes to reach 34psi
@01:42:43.78 Archive stroke #1 of 8
@01:42:43.88 Archive.in :pressurize
@01:42:43.95 Archive.seek :pressurized
@01:42:56.49 5.3psi after pressurize
@01:42:56.51 Archive.in :compress
@01:42:56.59 Archive.seek :empty
@01:43:11.22 8.8psi after compress
@01:43:41.30 15.4psi after compress (stable)
@01:43:41.32 Archive.in :gulp
@01:43:41.41 Archive.seek :topVent
@01:43:47.65 15.5psi after gulp
@01:43:53.25 15.5psi after gulp (stable)
@01:43:53.26 Archive stroke #2 of 8
@01:43:53.36 Archive.in :pressurize
@01:43:53.43 Archive.seek :pressurized
@01:44:05.92 15.6psi after pressurize
@01:44:05.94 Archive.in :compress
@01:44:06.02 Archive.seek :empty
@01:44:20.67 18.5psi after compress
@01:45:13.86 Reached 33.6 of 33.6psi after pumping 3460uL (2.00 strokes)
@01:45:13.87 Archive.in :default
@01:45:13.96 Archive.hold
@01:45:14.03 Handoff.select :waste
@01:45:21.23 delay 1:00
@01:46:21.24 Handoff.select :blocked
@01:46:48.98 Pressure too High, open to waste again (8.1psi)
@01:46:48.99 Handoff.select :waste
@01:46:49.14 delay 1:00
@01:47:49.15 Handoff.select :blocked
@01:48:26.83 Handoff.select :waste
@01:48:26.95 5.6psi at evacEnd
@01:48:26.97 Air.seek :fullVent
@01:48:28.42 Archive.in :fast
@01:48:28.51 Archive.seek :topVent
@01:48:35.61 Seawater evacuated, Pressure above/below filter released.
@01:48:35.70 Lysis1.in :slow
@01:48:35.77 Lysis1.seek 1050uL
@01:49:05.83 delay 5 seconds
@01:49:10.84 Handoff.select :blocked
@01:49:11.05 Lysis1.seek 450uL
@01:50:11.07 delay 3 seconds
@01:50:14.15 Air.in mix with 1.40048 parts Lysis1
@01:50:14.64 <Airmixer> Air.seek :evac
@01:50:14.68 <PROCESSING> Lysis1.seek 300uL
@01:50:29.81 Lysis1.seek :empty
@01:50:59.92 Lysis buffer delivered @28.8psi
@01:50:59.94 Polling Can every 2:00
@01:51:00.48 PuckHeater.configure :default #ambient 11.8C
@01:51:00.54 PuckHeater.seek 80.2C
@01:52:14.02 PuckHeater.hold 2:00,80.2C
@01:54:14.03 Polling Can every 2:00
@01:54:14.59 PuckHeater.configure :default #ambient 11.9C
@01:54:14.67 PuckHeater.hold 30 seconds,79.2C
@01:54:44.87 Polling Can every 2:00
@01:54:45.42 PuckHeater.configure :default #ambient 11.9C
@01:54:45.48 PuckHeater.hold 30 seconds,78.6C
@01:55:15.66 Polling Can every 2:00
@01:55:16.20 PuckHeater.configure :default #ambient 11.9C
@01:55:16.26 PuckHeater.hold 10:00,78.1C
@02:05:16.24 Lysis complete. Syringe pressure @24.0psi
@02:05:16.26 Archive syringe pumping up to 5 strokes to reach 40psi
@02:05:16.27 Archive stroke #1 of 5
@02:05:16.37 Archive.in :pressurize
@02:05:16.44 Archive.seek :pressurized
@02:05:28.93 23.5psi after pressurize
@02:05:34.52 23.3psi after pressurize (stable)
@02:05:34.54 Archive.in :compress
@02:05:34.64 Archive.seek :empty
@02:05:42.34 Reached 40.7 of 40.0psi after pumping 1580uL (0.91 strokes)
@02:05:42.36 Archive.in :default
@02:05:42.43 Archive.hold
@02:05:42.50 delay 2 seconds
@02:05:44.53 Archive.in :fast
@02:05:44.59 Archive.seek :full
@02:05:51.23 Lysis1.seek 138uL
@02:06:05.11 delay 1 second
@02:06:06.13 <fillArchive> Archive.seek :full
@02:06:06.16 <PROCESSING> Lysis1.seek :empty
@02:06:20.11 Handoff.select :blocked
@02:06:20.21 Initial air pressure @46.0psi
@02:06:20.28 Lysis1.in :extract
@02:06:20.37 Lysis1.seek 600uL,nil
@02:06:22.60 <pump> Archive syringe pumping up to 6 strokes to reach 25psi
@02:06:22.61 Archive stroke #1 of 6
@02:06:22.75 Archive.in :pressurize
@02:06:22.82 Archive.seek :pressurized
@02:06:35.68 15.7psi after pressurize
@02:06:41.30 15.7psi after pressurize (stable)
@02:06:41.32 Archive.in :compress
@02:06:41.40 Archive.seek :empty
@02:06:49.75 Archive.in :default
@02:06:49.81 Archive.hold
@02:06:49.95 <PROCESSING> Initial 600uL lysate @15.9psi
@02:06:49.96 Handoff.select :blocked
@02:06:50.06 Initial air pressure @16.7psi
@02:06:50.14 Lysis1.seek 825uL,nil
@02:06:50.87 <pump> Archive syringe pumping up to 6 strokes to reach 17psi
@02:06:50.88 Archive stroke #1 of 6
@02:06:50.98 Archive.in :compress
@02:06:51.07 Archive.seek :empty
@02:06:52.39 Reached 17.4 of 17.0psi after pumping 1623uL (0.94 strokes)
@02:06:52.40 Archive.in :default
@02:06:52.48 Archive.hold
@02:06:53.29 Archive syringe pumping up to 6 strokes to reach 17psi
@02:06:53.31 Archive stroke #1 of 6
@02:06:53.40 Archive.in :compress
@02:06:53.47 Archive.seek :empty
@02:06:55.46 Reached 17.1 of 17.0psi after pumping 1663uL (0.96 strokes)
@02:06:55.47 Archive.in :default
@02:06:55.55 Archive.hold
@02:06:56.34 Archive syringe pumping up to 6 strokes to reach 17psi
@02:06:56.35 Archive stroke #1 of 6
@02:06:56.45 Archive.in :compress
@02:06:56.52 Archive.seek :empty
@02:06:59.35 Archive.in :default
@02:06:59.41 Archive.hold
@02:06:59.62 <PROCESSING> Recovered 825uL lysate @15.0psi
@02:06:59.64 Air.in :halfSpeed
@02:06:59.72 Air.seek :fullVent
@02:07:01.93 Puck Top Vented, bottom @14.7psi
@02:07:01.94 Handoff.select :waste
@02:07:02.07 delay 5 seconds
@02:07:07.13 Handoff open to waste, bottom @-0.1psi
@02:07:07.15 Air.in :default
@02:07:07.23 Air.seek :evac
@02:07:08.65 Lysis1.in :DAmix
@02:07:08.77 Lysis2.in :DAmix
@02:07:09.28 <Lysis1mixer> Lysis1.seek 575uL
@02:07:09.33 <PROCESSING> Lysis2.seek 1480uL
@02:07:44.44 delay 5 seconds
@02:07:49.46 Handoff.select SPR
@02:07:49.59 SPR.command "start 2 14"
@02:07:50.39 SPR stabilized
@02:07:50.52 delay 10 seconds
@02:08:00.54 SPR.command "start 1 0"
@02:08:03.79 Waiting up to 4:00 for SPR to enter state 1
@02:10:08.01 SPR.command "hold_on"
@02:10:09.43 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2
@02:10:09.89 <Lysis1mixer> Lysis1.seek 275uL
@02:10:09.96 <PROCESSING> Lysis2.seek 1180uL
@02:10:52.01 delay 15 seconds
@02:11:07.03 SPR.command "hold_off"
@02:11:10.56 Waiting up to 38:00 for SPR to enter state 7
@02:29:10.61 <SPRlogger> SPRsummary:19.3RIU,148RIU,170RIU,224RIU,3.76ng/L,none,3.4ng/L after 3311s
@02:35:47.83 <PROCESSING> SPR analysis completed
@02:35:54.04 <SPRlogger> Ended log spr23apr1901h.spr.bz2
@02:35:54.05 <PROCESSING> SPR.command "stats_off"
@02:35:55.31 <LRAUV> -> Cmd.stop
@02:35:55.41 <closeHandoff> Handoff.select :close
@02:35:55.55 <LRAUV> Gate.power :heat,:OFF
@02:35:55.57 Gate.power :cartridge,:OFF
@02:35:55.66 PinPort.in :unlocking
@02:35:55.73 PinPort.seek :clear
@02:35:56.20 Carousel.in :default
@02:35:56.32 Carousel.home.seek 37
@02:35:56.62 Plunge.seek :locked
@02:35:57.50 <Archive> Archive.in :unlocking
@02:35:57.62 Archive.seek :clear
@02:35:57.70 <Air> Air.in :unlocking
@02:35:57.85 <Lysis1> Lysis1.in :unlocking
@02:35:57.88 <Air> Air.seek :clear
@02:35:58.04 <Lysis1> Lysis1.seek :clear
@02:35:58.11 <Lysis2> Lysis2.in :unlocking
@02:35:58.20 Lysis2.seek :clear
@02:36:02.18 <LRAUV> Gate.power :motor,:OFF
@02:36:02.20 Gate.power :core,:OFF
@02:36:02.22 Gate.power :analytic1,:OFF
@02:36:02.25 Gate.power :analytic2,:OFF
@02:36:02.27 Gate.power :analytic3,:OFF
@02:36:02.29 Gate.power :main,:OFF
@02:36:02.32 Safely stopped and ready to power off
:STOPPED