@15:40:45.16PDT20-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @15:40:45.20 >BREAK< ESP3 Gateway v1.42 for cartridge bus @15:40:47.95 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @15:40:48.42 Sea pressure (Abs) tare is 6% of full scale @15:40:53.99 Polling Can every 2:00 @15:40:54.47 Clients may connect on TCP port 9999 Connecting to 10.89.5.2:9999 @15:40:54.51 Waiting for espclients... @15:40:54.77 Session Begun @15:40:56.71 -> showStatus true @15:40:58.73 -> Cmd.loadCartridge -10 @15:40:58.80 Gate.power :main,:ON,limit to 5000mA @15:40:58.82 Gate.power :analytic1,:ON,limit to 5000mA @15:40:58.84 Gate.power :analytic2,:ON,limit to 5000mA @15:40:58.87 Gate.power :analytic3,:ON,limit to 5000mA @15:40:59.01 Powering core bus @15:41:01.27 SPR Module 1, FW Rev. B, FPGA Rev. C @15:41:01.71 Archive.configure :default @15:41:01.81 Air.configure :default @15:41:01.88 Lysis1.configure :default @15:41:01.94 Lysis2.configure :default @15:41:02.03 Plunge.configure :default @15:41:02.12 SamplePump.configure :default @15:41:02.21 Toroid.configure :default @15:41:02.30 Carousel.configure :default @15:41:02.39 Pressure.configure :default @15:41:02.48 ExtraPump.configure :default @15:41:02.55 PinPort.configure :default @15:41:02.67 All elf controllers running firmware version 4.83 @15:41:02.70 Gate.power :motor,:ON,limit to 5000mA @15:41:02.94 Archive.home.jog 0 @15:41:03.39 Carousel.home.jog 0 @15:41:03.44 Toroid.home.jog 0 @15:41:03.46 Lysis1.home.jog 0 @15:41:03.50 Lysis2.home.jog 0 @15:41:03.56 PinPort.home.jog 0 @15:41:03.58 Air.home.jog 0 @15:41:03.63 Plunge.home.jog 0 @15:41:04.19 Archive.in :homing @15:41:04.41 Plunge.in :homing @15:41:04.44 Archive.jog 1389 @15:41:04.47 PinPort.in :homing @15:41:04.50 Lysis2.in :homing @15:41:04.54 Air.in :homing @15:41:04.58 Toroid.in :homing @15:41:04.62 Lysis1.in :homing @15:41:04.86 Plunge.jog 1550 @15:41:04.89 PinPort.jog -104 @15:41:04.93 Lysis2.jog 1404 @15:41:04.96 Toroid.jog 615 @15:41:05.01 Lysis1.jog 158 @15:41:05.06 Air.jog 1386 @15:41:05.34 PinPort.setRawPosition! 0 @15:41:05.38 PinPort.seek :home @15:41:05.47 PinPort.in :default @15:41:05.60 Lysis1.setRawPosition! 50 @15:41:05.67 Lysis1.seek :home @15:41:05.86 Archive.setRawPosition! 50 @15:41:05.91 Archive.seek :home @15:41:05.98 Lysis1.in :default @15:41:06.23 Plunge.setRawPosition! 50 @15:41:06.25 Archive.in :default @15:41:06.35 Plunge.seek :home @15:41:06.81 Air.setRawPosition! 50 @15:41:06.83 Lysis2.setRawPosition! 50 @15:41:06.90 Air.seek :home @15:41:06.95 Lysis2.seek :home @15:41:07.09 Plunge.in :default @15:41:07.21 Toroid.setRawPosition! 0 @15:41:07.28 Toroid.seek :home @15:41:07.37 Lysis2.in :default @15:41:07.41 Air.in :default @15:41:07.44 Toroid.in :default @15:41:07.84 Toroid.seek :clear @15:41:09.47 Carousel.in :default @15:41:09.59 Carousel.home.seek 14 @15:41:09.84 Selecting Cartridge 25 @15:41:10.11 Carousel.seek 25 @15:41:12.63 Lysis1.in :fast @15:41:12.69 Lysis1.seek :locked @15:41:15.29 Plunge.seek :locked @15:41:15.43 Air.in :fast @15:41:15.50 Air.seek :locked @15:41:17.07 Carousel.reconfigure :locked @15:41:17.22 Powering cartridge bus @15:41:19.85 Gate.setIR 1750,5000,0,6 @15:41:19.97 Gate.setIR 0,5000,0,6 @15:41:20.05 PuckHeater.configure :default @15:41:20.11 Gate.power :heat,:ON,limit to 5000mA @15:41:20.81 Mounted lysis Cartridge board#Q05061 @15:41:20.86 Using this cartridge's heat loss curve for HeatingAccelInCarousel 25 @15:41:36.30 -> Cmd.startFiltering @15:41:36.55 Preparing to filter cartridge 25 92.07 @15:41:36.83 Priming sample loop w/100ml, bypass w/10ml @15:41:36.85 SamplePump.setPosition! 0ml @15:41:36.90 Bypass.open @15:41:38.38 Intake.open @15:41:39.19 Sea 10.1m deep (29.6psia) @15:41:39.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:41:41.80 Vacuum tare is 60% of full scale @15:41:41.93 Pressure.in :default @15:41:42.00 Pressure.hold @15:41:42.18 SamplePump.in :prime @15:41:42.26 SamplePump.seek 10ml,59 seconds @15:41:51.02 Pressure.stop @15:41:51.07 SamplePump.setPosition! 0ml @15:41:51.13 Exhaust.open @15:41:52.63 Bypass.close @15:41:54.12 Pressure.in :default @15:41:54.19 Pressure.hold @15:41:54.31 Sea 9.9m deep (29.3psia) @15:41:54.45 SamplePump.in :prime @15:41:54.53 SamplePump.seek 100ml,5:16 @15:43:31.23 Pressure.stop @15:43:31.31 Bypass.open @15:43:32.01 Intake.close @15:43:32.77 Toroid.in :hold @15:43:32.85 Toroid.seek :crack @15:43:34.38 Toroid.seek :cartridge @15:43:35.48 Toroid.in :default @15:43:35.54 Toroid.seek :clear @15:43:37.51 Pressurizing cartridge to 26psi above 29.5psia @15:43:37.53 SamplePump.setPosition! 0ml @15:43:37.59 Intake.open @15:43:38.23 Bypass.close @15:43:38.94 Exhaust.close @15:43:39.78 Pressure.in :default @15:43:39.85 Pressure.hold @15:43:40.04 SamplePump.in :charge @15:43:40.11 SamplePump.seek 2.3ml,42 seconds @15:43:48.82 Pressure.stop @15:43:48.88 Intake.close @15:43:49.26 Pressurized cartridge with 0.358ml to 26.1psi @15:43:49.27 Isolated @53.7psia (24.3psi) @15:43:49.30 Verifying cartridge holds at least 0.65..0.85 of 53.7psia @15:43:49.38 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:43:54.03 Sampling 1000.0ml in 1:25:20 @15:43:54.05 SamplePump.setPosition! 0ml @15:43:54.11 Exhaust.open @15:43:54.85 Intake.open @15:43:55.68 Pressure.in :default @15:43:55.75 Pressure.hold @15:43:55.87 Sea 10.6m deep (30.4psia) @15:43:55.88 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @15:43:56.04 SamplePump.in :sample @15:43:56.11 SamplePump.seek 1000ml,1:23:51 @15:43:58.72 Cartridge 25 filtering 1/1000ml at 0.44ml/s with 23.6psi vacuum 11.8m deep (32.1psia) @15:44:58.72 Cartridge 25 filtering 25/1000ml at 0.42ml/s with 25.4psi vacuum 9.6m deep (28.9psia) @15:45:58.72 Cartridge 25 filtering 48/1000ml at 0.40ml/s with 23.9psi vacuum 10.8m deep (30.7psia) @15:46:58.72 Cartridge 25 filtering 72/1000ml at 0.36ml/s with 22.5psi vacuum 8.7m deep (27.5psia) @15:47:58.73 Cartridge 25 filtering 94/1000ml at 0.37ml/s with 21.0psi vacuum 10.0m deep (29.4psia) @15:48:58.72 Cartridge 25 filtering 117/1000ml at 0.38ml/s with 24.5psi vacuum 9.1m deep (28.2psia) @15:49:58.72 Cartridge 25 filtering 139/1000ml at 0.34ml/s with 23.4psi vacuum 8.4m deep (27.0psia) @15:50:58.72 Cartridge 25 filtering 160/1000ml at 0.35ml/s with 21.0psi vacuum 9.5m deep (28.7psia) @15:51:58.72 Cartridge 25 filtering 181/1000ml at 0.35ml/s with 24.6psi vacuum 9.4m deep (28.5psia) @15:52:58.73 Cartridge 25 filtering 202/1000ml at 0.33ml/s with 22.7psi vacuum 9.7m deep (29.0psia) @15:53:58.72 Cartridge 25 filtering 223/1000ml at 0.33ml/s with 25.8psi vacuum 7.7m deep (26.1psia) @15:54:58.72 Cartridge 25 filtering 243/1000ml at 0.35ml/s with 24.7psi vacuum 10.2m deep (29.7psia) @15:55:58.72 Cartridge 25 filtering 263/1000ml at 0.34ml/s with 24.7psi vacuum 7.6m deep (26.0psia) @15:56:58.72 Cartridge 25 filtering 283/1000ml at 0.33ml/s with 25.7psi vacuum 8.6m deep (27.4psia) @15:57:58.72 Cartridge 25 filtering 302/1000ml at 0.30ml/s with 23.4psi vacuum 10.1m deep (29.6psia) @15:58:58.72 Cartridge 25 filtering 322/1000ml at 0.33ml/s with 22.9psi vacuum 8.4m deep (27.0psia) @15:59:58.73 Cartridge 25 filtering 340/1000ml at 0.29ml/s with 24.0psi vacuum 8.3m deep (27.0psia) @16:00:58.73 Cartridge 25 filtering 359/1000ml at 0.31ml/s with 26.3psi vacuum 8.5m deep (27.3psia) @16:01:58.73 Cartridge 25 filtering 378/1000ml at 0.31ml/s with 26.6psi vacuum 8.1m deep (26.6psia) @16:02:58.73 Cartridge 25 filtering 396/1000ml at 0.28ml/s with 26.4psi vacuum 6.8m deep (24.8psia) @16:03:58.72 Cartridge 25 filtering 414/1000ml at 0.31ml/s with 23.1psi vacuum 9.0m deep (27.9psia) @16:04:58.73 Cartridge 25 filtering 431/1000ml at 0.29ml/s with 22.4psi vacuum 8.8m deep (27.8psia) @16:05:58.73 Cartridge 25 filtering 449/1000ml at 0.29ml/s with 23.6psi vacuum 8.2m deep (26.8psia) @16:06:58.73 Cartridge 25 filtering 466/1000ml at 0.30ml/s with 23.4psi vacuum 10.6m deep (30.3psia) @16:07:58.72 Cartridge 25 filtering 483/1000ml at 0.28ml/s with 22.5psi vacuum 7.9m deep (26.4psia) @16:08:58.73 Cartridge 25 filtering 500/1000ml at 0.28ml/s with 21.1psi vacuum 9.3m deep (28.5psia) @16:09:58.72 Cartridge 25 filtering 517/1000ml at 0.27ml/s with 25.9psi vacuum 8.7m deep (27.5psia) @16:10:58.73 Cartridge 25 filtering 533/1000ml at 0.27ml/s with 24.6psi vacuum 8.6m deep (27.4psia) @16:11:58.72 Cartridge 25 filtering 550/1000ml at 0.26ml/s with 24.0psi vacuum 8.1m deep (26.7psia) @16:12:58.73 Cartridge 25 filtering 566/1000ml at 0.26ml/s with 26.4psi vacuum 8.5m deep (27.2psia) @16:13:58.72 Cartridge 25 filtering 582/1000ml at 0.25ml/s with 25.4psi vacuum 8.4m deep (27.1psia) @16:14:58.72 Cartridge 25 filtering 597/1000ml at 0.28ml/s with 25.2psi vacuum 8.7m deep (27.5psia) @16:15:58.74 Cartridge 25 filtering 613/1000ml at 0.26ml/s with 25.4psi vacuum 8.2m deep (26.8psia) @16:16:58.72 Cartridge 25 filtering 628/1000ml at 0.26ml/s with 24.3psi vacuum 7.8m deep (26.2psia) @16:17:58.72 Cartridge 25 filtering 643/1000ml at 0.25ml/s with 23.6psi vacuum 8.8m deep (27.7psia) @16:18:58.72 Cartridge 25 filtering 659/1000ml at 0.25ml/s with 25.6psi vacuum 8.4m deep (27.1psia) @16:19:58.72 Cartridge 25 filtering 673/1000ml at 0.25ml/s with 25.4psi vacuum 8.5m deep (27.2psia) @16:20:58.72 Cartridge 25 filtering 688/1000ml at 0.23ml/s with 24.5psi vacuum 8.1m deep (26.7psia) @16:21:58.72 Cartridge 25 filtering 703/1000ml at 0.23ml/s with 24.0psi vacuum 8.5m deep (27.3psia) @16:22:59.05 Cartridge 25 filtering 717/1000ml at 0.26ml/s with 23.6psi vacuum 10.0m deep (29.4psia) @16:23:58.73 Cartridge 25 filtering 731/1000ml at 0.25ml/s with 23.6psi vacuum 9.3m deep (28.4psia) @16:24:58.72 Cartridge 25 filtering 745/1000ml at 0.22ml/s with 23.7psi vacuum 8.9m deep (27.9psia) @16:25:58.73 Cartridge 25 filtering 759/1000ml at 0.24ml/s with 22.8psi vacuum 8.1m deep (26.7psia) @16:26:58.72 Cartridge 25 filtering 773/1000ml at 0.22ml/s with 23.4psi vacuum 9.3m deep (28.5psia) @16:27:58.73 Cartridge 25 filtering 787/1000ml at 0.25ml/s with 23.8psi vacuum 8.8m deep (27.7psia) @16:28:58.72 Cartridge 25 filtering 800/1000ml at 0.24ml/s with 22.6psi vacuum 8.7m deep (27.5psia) @16:29:58.72 Cartridge 25 filtering 814/1000ml at 0.22ml/s with 24.4psi vacuum 8.0m deep (26.5psia) @16:30:58.72 Cartridge 25 filtering 827/1000ml at 0.21ml/s with 24.5psi vacuum 8.5m deep (27.2psia) @16:31:58.72 Cartridge 25 filtering 840/1000ml at 0.24ml/s with 24.4psi vacuum 9.3m deep (28.4psia) @16:32:58.72 Cartridge 25 filtering 853/1000ml at 0.21ml/s with 25.3psi vacuum 8.8m deep (27.7psia) @16:33:58.72 Cartridge 25 filtering 866/1000ml at 0.21ml/s with 24.8psi vacuum 9.1m deep (28.2psia) @16:34:58.72 Cartridge 25 filtering 879/1000ml at 0.21ml/s with 26.6psi vacuum 8.5m deep (27.3psia) @16:35:58.72 Cartridge 25 filtering 892/1000ml at 0.20ml/s with 25.0psi vacuum 8.1m deep (26.7psia) @16:36:58.72 Cartridge 25 filtering 905/1000ml at 0.20ml/s with 24.7psi vacuum 9.0m deep (28.1psia) @16:37:58.72 Cartridge 25 filtering 917/1000ml at 0.22ml/s with 23.8psi vacuum 8.6m deep (27.4psia) @16:38:58.72 Cartridge 25 filtering 929/1000ml at 0.20ml/s with 25.6psi vacuum 9.3m deep (28.4psia) @16:39:58.72 Cartridge 25 filtering 942/1000ml at 0.20ml/s with 26.8psi vacuum 7.5m deep (25.8psia) @16:40:58.72 Cartridge 25 filtering 954/1000ml at 0.20ml/s with 25.3psi vacuum 9.0m deep (28.0psia) @16:41:58.72 Cartridge 25 filtering 966/1000ml at 0.21ml/s with 24.2psi vacuum 7.9m deep (26.4psia) @16:42:58.72 Cartridge 25 filtering 978/1000ml at 0.20ml/s with 25.2psi vacuum 9.0m deep (28.1psia) @16:43:58.72 Cartridge 25 filtering 991/1000ml at 0.20ml/s with 25.7psi vacuum 7.9m deep (26.4psia) @16:44:44.74 Pressure.stop @16:44:44.86 Intake.close @16:44:44.90 Exhaust.close @16:44:45.08 Toroid.in :hold @16:44:45.16 Toroid.seek :bypass @16:44:46.79 Toroid.in :default @16:44:46.86 Toroid.seek :clear @16:44:48.47 Sampled 1000.0ml @16:44:49.95 -> Cmd.startProcessing 120.0 @16:44:51.40 SPR.command "read_sequences" @16:44:51.76 SPR.command "stats_on" @16:44:51.89 SPR.command "start 0 1" @16:44:52.62 SPR.start /var/log/esp/spr23apr2016h.spr.bz2 @16:44:54.61 Handoff.select :waste @16:45:01.31 Syringe tare is 17% of full scale @16:45:01.34 PinPort.in :locking @16:45:01.42 PinPort.seek :locked @16:45:01.89 Archive.in :fast @16:45:01.95 Lysis2.in :fast @16:45:02.02 Lysis1.seek 1350uL @16:45:02.12 Archive.seek :full @16:45:02.20 Lysis2.seek :full @16:45:04.16 Plunge.seek :reagent @16:45:05.04 Evacuating puck with filter bubble point of 50psi @16:45:05.06 PinPort.seek :locked @16:45:05.15 Handoff.select :waste @16:45:11.73 Air syringe pumping up to 1 strokes to reach 8psi @16:45:11.74 Air stroke #1 of 1 @16:45:11.84 Air.in :pressurize @16:45:11.91 Air.seek :pressurized @16:45:17.65 0.4psi after pressurize @16:45:17.66 Air.in :compress @16:45:17.75 Air.seek :evac @16:45:20.78 0.6psi after compress @16:45:31.35 0.0psi after compress (stable) @16:45:31.37 Air.in :default @16:45:31.44 Air.hold @16:45:31.67 at -0.0psi Opened to waste @16:45:31.68 Handoff.select :blocked @16:45:31.83 Archive syringe pumping up to 8 strokes to reach 50psi @16:45:31.84 Archive stroke #1 of 8 @16:45:31.94 Archive.in :pressurize @16:45:32.05 Archive.seek :pressurized @16:45:44.56 7.6psi after pressurize @16:46:17.15 20.0psi after pressurize (stable) @16:46:17.16 Archive.in :compress @16:46:17.24 Archive.seek :empty @16:46:31.99 28.7psi after compress @16:47:02.08 35.3psi after compress (stable) @16:47:02.10 Archive.in :gulp @16:47:02.18 Archive.seek :topVent @16:47:08.44 35.9psi after gulp @16:47:14.03 35.8psi after gulp (stable) @16:47:14.04 Archive stroke #2 of 8 @16:47:14.17 Archive.in :pressurize @16:47:14.24 Archive.seek :pressurized @16:47:26.74 35.0psi after pressurize @16:47:32.32 35.5psi after pressurize (stable) @16:47:32.34 Archive.in :compress @16:47:32.42 Archive.seek :empty @16:47:46.60 Reached 50.3 of 50.0psi after pumping 3450uL (1.99 strokes) @16:47:46.61 Archive.in :default @16:47:46.69 Archive.hold @16:47:46.76 Handoff.select :waste @16:47:54.96 delay 1:00 @16:48:54.98 Handoff.select :blocked @16:49:22.67 Handoff.select :waste @16:49:22.81 4.3psi at evacEnd @16:49:22.82 Handoff.select :blocked @16:49:22.96 Archive.in :fast @16:49:23.02 Archive.seek :topVent @16:49:37.66 Stable @2.3psi after Handoff blocked @16:49:37.68 Archive syringe pumping up to 8 strokes to reach 32psi @16:49:37.69 Archive stroke #1 of 8 @16:49:37.80 Archive.in :pressurize @16:49:37.88 Archive.seek :pressurized @16:49:50.46 3.4psi after pressurize @16:49:50.48 Archive.in :compress @16:49:50.56 Archive.seek :empty @16:50:05.19 7.6psi after compress @16:50:38.77 17.3psi after compress (stable) @16:50:38.78 Archive.in :gulp @16:50:38.87 Archive.seek :topVent @16:50:45.13 18.3psi after gulp @16:50:50.73 18.8psi after gulp (stable) @16:50:50.77 Archive stroke #2 of 8 @16:50:50.87 Archive.in :pressurize @16:50:50.95 Archive.seek :pressurized @16:51:03.45 19.4psi after pressurize @16:51:03.46 Archive.in :compress @16:51:03.55 Archive.seek :empty @16:51:18.22 22.0psi after compress @16:51:47.40 Reached 32.3 of 32.3psi after pumping 3460uL (2.00 strokes) @16:51:47.41 Archive.in :default @16:51:47.52 Archive.hold @16:51:47.59 Handoff.select :waste @16:51:58.28 delay 1:00 @16:52:58.30 Handoff.select :blocked @16:53:21.02 Handoff.select :waste @16:53:21.16 2.0psi at evacEnd @16:53:21.18 Air.seek :fullVent @16:53:22.61 Archive.in :fast @16:53:22.68 Archive.seek :topVent @16:53:29.79 Seawater evacuated, Pressure above/below filter released. @16:53:29.88 Lysis1.in :slow @16:53:29.95 Lysis1.seek 1050uL @16:54:00.00 delay 5 seconds @16:54:05.02 Handoff.select :blocked @16:54:05.21 Lysis1.seek 450uL @16:55:05.52 delay 3 seconds @16:55:08.59 Air.in mix with 1.40048 parts Lysis1 @16:55:09.08 Air.seek :evac @16:55:09.12 Lysis1.seek 300uL @16:55:24.25 Lysis1.seek :empty @16:55:54.40 Lysis buffer delivered @19.9psi @16:55:54.42 Polling Can every 2:00 @16:55:54.97 PuckHeater.configure :default #ambient 12.9C @16:55:55.02 PuckHeater.seek 81.2C @16:57:08.28 PuckHeater.hold 2:00,81.2C @16:59:08.30 Polling Can every 2:00 @16:59:08.84 PuckHeater.configure :default #ambient 13.0C @16:59:08.90 PuckHeater.hold 30 seconds,80.1C @16:59:39.07 Polling Can every 2:00 @16:59:39.61 PuckHeater.configure :default #ambient 13.0C @16:59:39.67 PuckHeater.hold 30 seconds,79.6C @17:00:09.85 Polling Can every 2:00 @17:00:10.39 PuckHeater.configure :default #ambient 12.9C @17:00:10.45 PuckHeater.hold 10:00,79C @17:10:09.98 Lysis complete. Syringe pressure @26.7psi @17:10:10.01 Archive syringe pumping up to 5 strokes to reach 40psi @17:10:10.02 Archive stroke #1 of 5 @17:10:10.12 Archive.in :pressurize @17:10:10.19 Archive.seek :pressurized @17:10:22.69 26.4psi after pressurize @17:10:28.28 26.5psi after pressurize (stable) @17:10:28.32 Archive.in :compress @17:10:28.40 Archive.seek :empty @17:10:36.87 Reached 40.6 of 40.0psi after pumping 1596uL (0.92 strokes) @17:10:36.89 Archive.in :default @17:10:36.97 Archive.hold @17:10:37.05 delay 2 seconds @17:10:39.07 Archive.in :fast @17:10:39.13 Archive.seek :full @17:10:45.84 Lysis1.seek 138uL @17:10:59.72 delay 1 second @17:11:00.75 Archive.seek :full @17:11:00.78 Lysis1.seek :empty @17:11:14.68 Handoff.select :blocked @17:11:14.77 Initial air pressure @50.1psi @17:11:14.83 Lysis1.in :extract @17:11:14.95 Lysis1.seek 600uL,nil @17:11:17.17 Archive syringe pumping up to 6 strokes to reach 25psi @17:11:17.18 Archive stroke #1 of 6 @17:11:17.31 Archive.in :pressurize @17:11:17.39 Archive.seek :pressurized @17:11:29.94 15.6psi after pressurize @17:11:47.01 15.1psi after pressurize (stable) @17:11:47.03 Archive.in :compress @17:11:47.12 Archive.seek :empty @17:11:56.87 Archive.in :default @17:11:56.94 Archive.hold @17:11:57.08 Initial 600uL lysate @15.9psi @17:11:57.09 Handoff.select :blocked @17:11:57.22 Initial air pressure @16.5psi @17:11:57.69 Lysis1.seek 825uL,nil @17:11:58.38 Archive syringe pumping up to 6 strokes to reach 17psi @17:11:58.40 Archive stroke #1 of 6 @17:11:58.49 Archive.in :compress @17:11:58.59 Archive.seek :empty @17:12:00.22 Reached 17.0 of 17.0psi after pumping 1659uL (0.96 strokes) @17:12:00.24 Archive.in :default @17:12:00.31 Archive.hold @17:12:01.04 Archive syringe pumping up to 6 strokes to reach 17psi @17:12:01.05 Archive stroke #1 of 6 @17:12:01.19 Archive.in :compress @17:12:01.26 Archive.seek :empty @17:12:03.17 Reached 17.0 of 17.0psi after pumping 1697uL (0.98 strokes) @17:12:03.19 Archive.in :default @17:12:03.26 Archive.hold @17:12:03.99 Archive syringe pumping up to 6 strokes to reach 17psi @17:12:04.00 Archive stroke #1 of 6 @17:12:04.09 Archive.in :compress @17:12:04.17 Archive.seek :empty @17:12:05.39 Archive.in :default @17:12:05.45 Archive.hold @17:12:05.68 Recovered 825uL lysate @14.0psi @17:12:05.71 Air.in :halfSpeed @17:12:05.81 Air.seek :fullVent @17:12:08.01 Puck Top Vented, bottom @1.7psi @17:12:08.02 Handoff.select :waste @17:12:08.15 delay 5 seconds @17:12:13.22 Handoff open to waste, bottom @-0.0psi @17:12:13.25 Air.in :default @17:12:13.34 Air.seek :evac @17:12:14.76 Lysis2.in :DAmix @17:12:14.88 Lysis1.in :DAmix @17:12:15.39 Lysis2.seek 1480uL @17:12:15.42 Lysis1.seek 575uL @17:12:50.57 delay 5 seconds @17:12:55.59 Handoff.select SPR @17:12:55.70 SPR.command "start 2 14" @17:12:56.31 SPR stabilized @17:12:56.44 delay 10 seconds @17:13:06.46 SPR.command "start 1 0" @17:13:09.67 Waiting up to 4:00 for SPR to enter state 1 @17:15:13.92 SPR.command "hold_on" @17:15:15.34 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:15:15.81 Lysis2.seek 1180uL @17:15:15.89 Lysis1.seek 275uL @17:15:57.90 delay 15 seconds @17:16:12.91 SPR.command "hold_off" @17:16:16.00 Waiting up to 38:00 for SPR to enter state 7 @17:34:15.58 SPRsummary:15.2RIU,151RIU,161RIU,221RIU,2.75ng/L,none,3.15ng/L after 3130s @17:40:52.80 SPR analysis completed @17:40:58.41 Ended log spr23apr2016h.spr.bz2 @17:40:58.42 SPR.command "stats_off" @17:40:59.77 -> Cmd.stop @17:40:59.88 Handoff.select :close @17:41:00.01 Gate.power :heat,:OFF @17:41:00.03 Gate.power :cartridge,:OFF @17:41:00.12 PinPort.in :unlocking @17:41:00.19 PinPort.seek :clear @17:41:00.66 Carousel.in :default @17:41:00.78 Carousel.home.seek 25 @17:41:01.10 Plunge.seek :locked @17:41:01.97 Archive.in :unlocking @17:41:02.11 Air.in :unlocking @17:41:02.16 Archive.seek :clear @17:41:02.35 Air.seek :clear @17:41:02.46 Lysis1.in :unlocking @17:41:02.60 Lysis2.in :unlocking @17:41:02.64 Lysis1.seek :clear @17:41:02.75 Lysis2.seek :clear @17:41:06.73 Gate.power :motor,:OFF @17:41:06.75 Gate.power :core,:OFF @17:41:06.77 Gate.power :analytic1,:OFF @17:41:06.79 Gate.power :analytic2,:OFF @17:41:06.81 Gate.power :analytic3,:OFF @17:41:06.84 Gate.power :main,:OFF @17:41:06.86 Safely stopped and ready to power off :STOPPED