@08:24:27.84PDT21-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @08:24:27.88 >BREAK< ESP3 Gateway v1.42 for cartridge bus @08:24:30.56 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @08:24:31.03 Sea pressure (Abs) tare is 6% of full scale @08:24:36.61 Polling Can every 2:00 @08:24:37.08 Clients may connect on TCP port 9999 Connecting to 10.89.5.2:9999 @08:24:37.12 Waiting for espclients... @08:24:37.44 Session Begun @08:24:39.39 -> showStatus true @08:24:41.41 -> Cmd.loadCartridge -10 @08:24:41.47 Gate.power :main,:ON,limit to 5000mA @08:24:41.49 Gate.power :analytic1,:ON,limit to 5000mA @08:24:41.52 Gate.power :analytic2,:ON,limit to 5000mA @08:24:41.54 Gate.power :analytic3,:ON,limit to 5000mA @08:24:41.68 Powering core bus @08:24:43.95 SPR Module 1, FW Rev. B, FPGA Rev. C @08:24:44.38 Archive.configure :default @08:24:44.47 Air.configure :default @08:24:44.53 Lysis1.configure :default @08:24:44.60 Lysis2.configure :default @08:24:44.69 Plunge.configure :default @08:24:44.78 SamplePump.configure :default @08:24:44.87 Toroid.configure :default @08:24:44.96 Carousel.configure :default @08:24:45.05 Pressure.configure :default @08:24:45.14 ExtraPump.configure :default @08:24:45.21 PinPort.configure :default @08:24:45.28 All elf controllers running firmware version 4.83 @08:24:45.31 Gate.power :motor,:ON,limit to 5000mA @08:24:45.44 Archive.home.jog 0 @08:24:46.02 Carousel.home.jog 0 @08:24:46.04 Toroid.home.jog 0 @08:24:46.08 Lysis2.home.jog 0 @08:24:46.14 PinPort.home.jog 0 @08:24:46.17 Plunge.home.jog 0 @08:24:46.20 Air.home.jog 0 @08:24:46.25 Lysis1.home.jog 0 @08:24:46.80 Archive.in :homing @08:24:46.92 Archive.jog 1393 @08:24:47.11 PinPort.in :homing @08:24:47.15 Toroid.in :homing @08:24:47.18 Plunge.in :homing @08:24:47.22 Lysis2.in :homing @08:24:47.25 Lysis1.in :homing @08:24:47.29 Air.in :homing @08:24:47.50 PinPort.jog -105 @08:24:47.54 Toroid.jog 619 @08:24:47.59 Plunge.jog 1534 @08:24:47.62 Lysis2.jog 1407 @08:24:47.67 Air.jog 1378 @08:24:47.73 Lysis1.jog 157 @08:24:47.95 PinPort.setRawPosition! 0 @08:24:48.00 PinPort.seek :home @08:24:48.08 PinPort.in :default @08:24:48.16 Archive.setRawPosition! 50 @08:24:48.21 Archive.seek :home @08:24:48.29 Lysis1.setRawPosition! 50 @08:24:48.35 Lysis1.seek :home @08:24:48.49 Archive.in :default @08:24:49.02 Lysis2.setRawPosition! 50 @08:24:49.05 Air.setRawPosition! 50 @08:24:49.11 Plunge.setRawPosition! 50 @08:24:49.15 Lysis1.in :default @08:24:49.27 Lysis2.seek :home @08:24:49.33 Air.seek :home @08:24:49.39 Plunge.seek :home @08:24:49.63 Lysis2.in :default @08:24:49.72 Air.in :default @08:24:49.82 Plunge.in :default @08:24:49.84 Toroid.setRawPosition! 0 @08:24:49.93 Toroid.seek :home @08:24:50.03 Toroid.in :default @08:24:50.21 Toroid.seek :clear @08:24:51.83 Carousel.in :default @08:24:51.96 Carousel.home.seek 12 @08:24:52.21 Selecting Cartridge 21 @08:24:52.48 Carousel.seek 21 @08:24:54.61 Lysis1.in :fast @08:24:54.68 Lysis1.seek :locked @08:24:57.27 Plunge.seek :locked @08:24:57.41 Air.in :fast @08:24:57.48 Air.seek :locked @08:24:59.08 Carousel.reconfigure :locked @08:24:59.23 Powering cartridge bus @08:25:01.84 Gate.setIR 1750,5000,0,6 @08:25:01.97 Gate.setIR 0,5000,0,6 @08:25:02.03 PuckHeater.configure :default @08:25:02.10 Gate.power :heat,:ON,limit to 5000mA @08:25:02.81 Mounted lysis Cartridge board#Q05038 @08:25:02.84 Using default cartridge heat loss curve for HeatingAccelInCarousel 21 @08:25:18.98 -> Cmd.startFiltering @08:25:19.22 Preparing to filter cartridge 21 92.07 @08:25:19.51 Priming sample loop w/100ml, bypass w/10ml @08:25:19.52 SamplePump.setPosition! 0ml @08:25:19.58 Bypass.open @08:25:21.03 Intake.open @08:25:21.79 Sea 16.4m deep (39.0psia) @08:25:21.81 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @08:25:24.39 Vacuum tare is 60% of full scale @08:25:24.53 Intake.close @08:25:24.58 Bypass.close @08:25:25.30 Verifying toroid holds at least 0.6..0.85 of 39.3psia @08:25:25.33 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @08:25:29.90 Toroid pressure rose 2% to 40.3psia @08:25:29.91 Bypass.open @08:25:30.64 Intake.open @08:25:32.06 Pressure.in :default @08:25:32.13 Pressure.hold @08:25:32.32 SamplePump.in :prime @08:25:32.39 SamplePump.seek 10ml,59 seconds @08:25:41.14 Pressure.stop @08:25:41.20 SamplePump.setPosition! 0ml @08:25:41.26 Exhaust.open @08:25:42.76 Bypass.close @08:25:44.30 Pressure.in :default @08:25:44.36 Pressure.hold @08:25:44.49 Sea 16.0m deep (38.3psia) @08:25:44.63 SamplePump.in :prime @08:25:44.71 SamplePump.seek 100ml,5:16 @08:27:21.86 Pressure.stop @08:27:21.94 Bypass.open @08:27:22.69 Intake.close @08:27:23.48 Toroid.in :hold @08:27:23.54 Toroid.seek :crack @08:27:25.08 Toroid.seek :cartridge @08:27:26.19 Toroid.in :default @08:27:26.25 Toroid.seek :clear @08:27:28.22 Exhaust.close @08:27:28.25 Bypass.close @08:27:29.01 Verifying cartridge holds at least 0.65..0.85 of 38.2psia @08:27:29.03 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @08:27:33.67 Sampling 1000.0ml in 1:30:34.285714 @08:27:33.69 SamplePump.setPosition! 0ml @08:27:33.75 Exhaust.open @08:27:34.51 Intake.open @08:27:35.34 Pressure.in :default @08:27:35.41 Pressure.hold @08:27:35.53 Sea 15.7m deep (37.9psia) @08:27:35.54 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @08:27:35.70 SamplePump.in :sample @08:27:35.77 SamplePump.seek 1000ml,1:23:51 @08:27:38.38 Cartridge 21 filtering 1/1000ml at 0.43ml/s with 25.0psi vacuum 14.4m deep (36.0psia) @08:28:38.38 Cartridge 21 filtering 25/1000ml at 0.41ml/s with 25.0psi vacuum 15.9m deep (38.2psia) @08:29:38.39 Cartridge 21 filtering 48/1000ml at 0.41ml/s with 24.1psi vacuum 17.4m deep (40.4psia) @08:30:38.38 Cartridge 21 filtering 72/1000ml at 0.40ml/s with 24.8psi vacuum 15.1m deep (37.0psia) @08:31:38.39 Cartridge 21 filtering 95/1000ml at 0.40ml/s with 24.3psi vacuum 16.5m deep (39.2psia) @08:32:38.38 Cartridge 21 filtering 117/1000ml at 0.36ml/s with 24.0psi vacuum 14.8m deep (36.5psia) @08:33:38.39 Cartridge 21 filtering 140/1000ml at 0.38ml/s with 25.7psi vacuum 14.8m deep (36.5psia) @08:34:38.38 Cartridge 21 filtering 162/1000ml at 0.36ml/s with 21.5psi vacuum 15.4m deep (37.5psia) @08:35:38.38 Cartridge 21 filtering 184/1000ml at 0.35ml/s with 23.1psi vacuum 14.6m deep (36.2psia) @08:36:38.38 Cartridge 21 filtering 206/1000ml at 0.34ml/s with 24.5psi vacuum 15.0m deep (36.9psia) @08:37:38.38 Cartridge 21 filtering 227/1000ml at 0.35ml/s with 21.3psi vacuum 16.1m deep (38.5psia) @08:38:38.38 Cartridge 21 filtering 248/1000ml at 0.36ml/s with 23.1psi vacuum 15.2m deep (37.1psia) @08:39:38.38 Cartridge 21 filtering 269/1000ml at 0.34ml/s with 26.4psi vacuum 15.4m deep (37.4psia) @08:40:38.38 Cartridge 21 filtering 290/1000ml at 0.36ml/s with 25.0psi vacuum 15.3m deep (37.3psia) @08:41:38.38 Cartridge 21 filtering 310/1000ml at 0.35ml/s with 22.4psi vacuum 15.4m deep (37.4psia) @08:42:38.38 Cartridge 21 filtering 330/1000ml at 0.34ml/s with 25.8psi vacuum 15.6m deep (37.8psia) @08:43:38.39 Cartridge 21 filtering 350/1000ml at 0.32ml/s with 23.6psi vacuum 14.4m deep (35.9psia) @08:44:38.38 Cartridge 21 filtering 370/1000ml at 0.30ml/s with 24.9psi vacuum 13.3m deep (34.3psia) @08:45:38.38 Cartridge 21 filtering 389/1000ml at 0.31ml/s with 26.7psi vacuum 14.5m deep (36.1psia) @08:46:38.38 Cartridge 21 filtering 408/1000ml at 0.32ml/s with 22.0psi vacuum 14.1m deep (35.5psia) @08:47:38.43 Cartridge 21 filtering 427/1000ml at 0.31ml/s with 22.7psi vacuum 15.9m deep (38.2psia) @08:48:38.38 Cartridge 21 filtering 446/1000ml at 0.33ml/s with 23.2psi vacuum 14.1m deep (35.6psia) @08:49:38.38 Cartridge 21 filtering 465/1000ml at 0.30ml/s with 22.1psi vacuum 15.9m deep (38.2psia) @08:50:38.38 Cartridge 21 filtering 483/1000ml at 0.30ml/s with 22.5psi vacuum 14.0m deep (35.5psia) @08:51:38.39 Cartridge 21 filtering 502/1000ml at 0.30ml/s with 25.4psi vacuum 15.0m deep (36.9psia) @08:52:38.38 Cartridge 21 filtering 520/1000ml at 0.29ml/s with 25.3psi vacuum 15.1m deep (37.0psia) @08:53:38.38 Cartridge 21 filtering 538/1000ml at 0.28ml/s with 22.8psi vacuum 14.3m deep (35.8psia) @08:54:38.38 Cartridge 21 filtering 555/1000ml at 0.27ml/s with 25.2psi vacuum 13.4m deep (34.5psia) @08:55:38.39 Cartridge 21 filtering 573/1000ml at 0.28ml/s with 23.6psi vacuum 13.5m deep (34.6psia) @08:56:38.38 Cartridge 21 filtering 590/1000ml at 0.27ml/s with 25.2psi vacuum 13.4m deep (34.5psia) @08:57:38.38 Cartridge 21 filtering 608/1000ml at 0.29ml/s with 23.0psi vacuum 14.7m deep (36.4psia) @08:58:38.38 Cartridge 21 filtering 625/1000ml at 0.27ml/s with 24.4psi vacuum 13.4m deep (34.5psia) @08:59:38.38 Cartridge 21 filtering 642/1000ml at 0.30ml/s with 23.6psi vacuum 15.3m deep (37.3psia) @09:00:38.38 Cartridge 21 filtering 659/1000ml at 0.29ml/s with 25.5psi vacuum 14.7m deep (36.3psia) @09:01:38.39 Cartridge 21 filtering 675/1000ml at 0.29ml/s with 25.3psi vacuum 14.8m deep (36.6psia) @09:02:38.38 Cartridge 21 filtering 692/1000ml at 0.27ml/s with 22.6psi vacuum 16.5m deep (39.2psia) @09:03:38.68 Cartridge 21 filtering 709/1000ml at 0.27ml/s with 23.1psi vacuum 15.1m deep (37.1psia) @09:04:38.38 Cartridge 21 filtering 725/1000ml at 0.26ml/s with 24.3psi vacuum 13.0m deep (34.0psia) @09:05:38.39 Cartridge 21 filtering 741/1000ml at 0.29ml/s with 26.2psi vacuum 15.2m deep (37.1psia) @09:06:38.39 Cartridge 21 filtering 758/1000ml at 0.27ml/s with 24.8psi vacuum 15.2m deep (37.2psia) @09:07:38.39 Cartridge 21 filtering 774/1000ml at 0.28ml/s with 23.2psi vacuum 14.3m deep (35.8psia) @09:08:38.38 Cartridge 21 filtering 790/1000ml at 0.26ml/s with 24.9psi vacuum 14.8m deep (36.5psia) @09:09:38.39 Cartridge 21 filtering 806/1000ml at 0.25ml/s with 23.1psi vacuum 15.0m deep (36.9psia) @09:10:38.39 Cartridge 21 filtering 821/1000ml at 0.27ml/s with 27.0psi vacuum 13.8m deep (35.1psia) @09:11:38.38 Cartridge 21 filtering 837/1000ml at 0.26ml/s with 23.7psi vacuum 14.9m deep (36.6psia) @09:12:38.39 Cartridge 21 filtering 853/1000ml at 0.26ml/s with 23.6psi vacuum 14.0m deep (35.3psia) @09:13:38.39 Cartridge 21 filtering 868/1000ml at 0.26ml/s with 22.7psi vacuum 16.2m deep (38.7psia) @09:14:38.38 Cartridge 21 filtering 884/1000ml at 0.25ml/s with 24.6psi vacuum 12.9m deep (33.7psia) @09:15:38.40 Cartridge 21 filtering 899/1000ml at 0.26ml/s with 24.0psi vacuum 15.3m deep (37.3psia) @09:16:38.39 Cartridge 21 filtering 914/1000ml at 0.26ml/s with 22.2psi vacuum 14.8m deep (36.6psia) @09:17:38.39 Cartridge 21 filtering 929/1000ml at 0.25ml/s with 22.8psi vacuum 15.6m deep (37.8psia) @09:18:38.39 Cartridge 21 filtering 944/1000ml at 0.24ml/s with 23.9psi vacuum 14.9m deep (36.7psia) @09:19:38.39 Cartridge 21 filtering 959/1000ml at 0.25ml/s with 22.5psi vacuum 15.3m deep (37.2psia) @09:20:38.39 Cartridge 21 filtering 974/1000ml at 0.26ml/s with 22.1psi vacuum 16.6m deep (39.2psia) @09:21:38.38 Cartridge 21 filtering 989/1000ml at 0.25ml/s with 24.4psi vacuum 14.4m deep (35.9psia) @09:22:22.14 Pressure.stop @09:22:22.26 Intake.close @09:22:22.30 Exhaust.close @09:22:22.49 Toroid.in :hold @09:22:22.56 Toroid.seek :bypass @09:22:24.19 Toroid.in :default @09:22:24.26 Toroid.seek :clear @09:22:25.87 Sampled 1000.0ml @09:22:27.38 -> Cmd.startProcessing 120.0 @09:22:28.84 SPR.command "read_sequences" @09:22:29.35 SPR.command "stats_on" @09:22:29.48 SPR.command "start 0 1" @09:22:30.20 SPR.start /var/log/esp/spr23apr2109h.spr.bz2 @09:22:32.20 Handoff.select :waste @09:22:38.89 Syringe tare is 17% of full scale @09:22:38.91 PinPort.in :locking @09:22:38.99 PinPort.seek :locked @09:22:39.46 Archive.in :fast @09:22:39.50 Lysis2.in :fast @09:22:39.59 Lysis1.seek 1350uL @09:22:39.66 Archive.seek :full @09:22:39.76 Lysis2.seek :full @09:22:41.75 Plunge.seek :reagent @09:22:42.63 Evacuating puck with filter bubble point of 50psi @09:22:42.65 PinPort.seek :locked @09:22:42.74 Handoff.select :waste @09:22:49.33 Air syringe pumping up to 1 strokes to reach 8psi @09:22:49.34 Air stroke #1 of 1 @09:22:49.43 Air.in :pressurize @09:22:49.51 Air.seek :pressurized @09:22:55.25 0.1psi after pressurize @09:22:55.26 Air.in :compress @09:22:55.37 Air.seek :evac @09:22:58.40 0.8psi after compress @09:23:08.97 0.1psi after compress (stable) @09:23:08.98 Air.in :default @09:23:09.06 Air.hold @09:23:09.25 at 0.0psi Opened to waste @09:23:09.26 Handoff.select :blocked @09:23:09.40 Archive syringe pumping up to 8 strokes to reach 50psi @09:23:09.41 Archive stroke #1 of 8 @09:23:09.50 Archive.in :pressurize @09:23:09.62 Archive.seek :pressurized @09:23:22.12 8.7psi after pressurize @09:23:38.70 19.3psi after pressurize (stable) @09:23:38.72 Archive.in :compress @09:23:38.80 Archive.seek :empty @09:23:53.44 30.4psi after compress @09:24:04.02 31.4psi after compress (stable) @09:24:04.04 Archive.in :gulp @09:24:04.12 Archive.seek :topVent @09:24:10.37 31.5psi after gulp @09:24:16.16 31.4psi after gulp (stable) @09:24:16.17 Archive stroke #2 of 8 @09:24:16.28 Archive.in :pressurize @09:24:16.35 Archive.seek :pressurized @09:24:28.86 31.3psi after pressurize @09:24:47.44 36.0psi after pressurize (stable) @09:24:47.46 Archive.in :compress @09:24:47.55 Archive.seek :empty @09:25:01.33 Reached 50.2 of 50.0psi after pumping 3441uL (1.99 strokes) @09:25:01.35 Archive.in :default @09:25:01.43 Archive.hold @09:25:01.50 Handoff.select :waste @09:25:14.68 delay 1:00 @09:26:14.70 Handoff.select :blocked @09:26:37.38 Handoff.select :waste @09:26:37.50 1.9psi at evacEnd @09:26:37.51 Handoff.select :blocked @09:26:37.66 Archive.in :fast @09:26:37.73 Archive.seek :topVent @09:26:51.34 Stable @1.2psi after Handoff blocked @09:26:51.36 Archive syringe pumping up to 8 strokes to reach 31psi @09:26:51.38 Archive stroke #1 of 8 @09:26:51.49 Archive.in :pressurize @09:26:51.56 Archive.seek :pressurized @09:27:04.05 2.0psi after pressurize @09:27:04.07 Archive.in :compress @09:27:04.19 Archive.seek :empty @09:27:18.81 8.4psi after compress @09:27:41.88 15.5psi after compress (stable) @09:27:41.91 Archive.in :gulp @09:27:42.02 Archive.seek :topVent @09:27:48.26 16.4psi after gulp @09:27:53.84 16.7psi after gulp (stable) @09:27:53.85 Archive stroke #2 of 8 @09:27:53.96 Archive.in :pressurize @09:27:54.03 Archive.seek :pressurized @09:28:06.53 17.4psi after pressurize @09:28:06.56 Archive.in :compress @09:28:06.65 Archive.seek :empty @09:28:21.33 22.9psi after compress @09:28:45.91 30.0psi after compress (stable) @09:28:45.92 Archive.in :gulp @09:28:46.01 Archive.seek :topVent @09:28:52.26 30.9psi after gulp @09:28:56.07 Reached 31.3 of 31.2psi after pumping 3460uL (2.00 strokes) @09:28:56.09 Archive.in :default @09:28:56.17 Archive.hold @09:28:56.24 Handoff.select :waste @09:29:12.92 delay 1:00 @09:30:12.94 Handoff.select :blocked @09:30:35.63 Handoff.select :waste @09:30:35.75 0.8psi at evacEnd @09:30:35.77 Air.seek :fullVent @09:30:37.20 Archive.in :fast @09:30:37.27 Archive.seek :topVent @09:30:37.35 Seawater evacuated, Pressure above/below filter released. @09:30:37.44 Lysis1.in :slow @09:30:37.51 Lysis1.seek 1050uL @09:31:07.54 delay 5 seconds @09:31:12.56 Handoff.select :blocked @09:31:12.75 Lysis1.seek 450uL @09:32:12.73 delay 3 seconds @09:32:15.81 Air.in mix with 1.40048 parts Lysis1 @09:32:16.27 Air.seek :evac @09:32:16.33 Lysis1.seek 300uL @09:32:31.44 Lysis1.seek :empty @09:33:01.57 Lysis buffer delivered @35.2psi @09:33:01.58 Polling Can every 2:00 @09:33:02.14 PuckHeater.configure :default #ambient 11.5C @09:33:02.19 PuckHeater.seek 80.2C @09:34:14.24 PuckHeater.hold 2:00,80.2C @09:36:14.07 Polling Can every 2:00 @09:36:14.62 PuckHeater.configure :default #ambient 11.6C @09:36:14.69 PuckHeater.hold 30 seconds,79.2C @09:36:44.84 Polling Can every 2:00 @09:36:45.39 PuckHeater.configure :default #ambient 11.5C @09:36:45.44 PuckHeater.hold 30 seconds,78.7C @09:37:15.57 Polling Can every 2:00 @09:37:16.11 PuckHeater.configure :default #ambient 11.5C @09:37:16.17 PuckHeater.hold 10:00,78.1C @09:47:15.19 Lysis complete. Syringe pressure @39.2psi @09:47:15.21 Archive syringe pumping up to 5 strokes to reach 40psi @09:47:15.23 Archive stroke #1 of 5 @09:47:15.33 Archive.in :pressurize @09:47:15.40 Archive.seek :pressurized @09:47:27.90 38.6psi after pressurize @09:47:33.47 38.5psi after pressurize (stable) @09:47:33.49 Archive.in :compress @09:47:33.57 Archive.seek :empty @09:47:40.51 Reached 43.0 of 40.0psi after pumping 1564uL (0.90 strokes) @09:47:40.52 Archive.in :default @09:47:40.60 Archive.hold @09:47:40.67 delay 2 seconds @09:47:42.69 Archive.in :fast @09:47:42.76 Archive.seek :full @09:47:49.35 Lysis1.seek 138uL @09:48:03.24 delay 1 second @09:48:04.26 Archive.seek :full @09:48:04.30 Lysis1.seek :empty @09:48:18.24 Handoff.select :blocked @09:48:18.33 Initial air pressure @52.9psi @09:48:18.40 Lysis1.in :extract @09:48:18.49 Lysis1.seek 600uL,nil @09:48:20.19 Archive syringe pumping up to 6 strokes to reach 25psi @09:48:20.20 Archive stroke #1 of 6 @09:48:20.35 Archive.in :pressurize @09:48:20.42 Archive.seek :pressurized @09:48:32.91 13.9psi after pressurize @09:49:02.20 Archive.in :default @09:49:02.26 Archive.hold @09:49:02.39 Initial 600uL lysate @13.8psi @09:49:02.40 Handoff.select :blocked @09:49:02.51 Initial air pressure @14.2psi @09:49:02.60 Lysis1.seek 825uL,nil @09:49:02.71 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:02.72 Archive stroke #1 of 6 @09:49:02.87 Archive.in :compress @09:49:02.96 Archive.seek :empty @09:49:12.24 Reached 17.1 of 17.0psi after pumping 1614uL (0.93 strokes) @09:49:12.25 Archive.in :default @09:49:12.32 Archive.hold @09:49:13.08 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:13.09 Archive stroke #1 of 6 @09:49:13.19 Archive.in :compress @09:49:13.27 Archive.seek :empty @09:49:15.13 Reached 16.9 of 17.0psi after pumping 1650uL (0.95 strokes) @09:49:15.14 Archive.in :default @09:49:15.23 Archive.hold @09:49:16.01 Archive syringe pumping up to 6 strokes to reach 17psi @09:49:16.02 Archive stroke #1 of 6 @09:49:16.12 Archive.in :compress @09:49:16.19 Archive.seek :empty @09:49:18.37 Archive.in :default @09:49:18.45 Archive.hold @09:49:18.72 Recovered 825uL lysate @15.8psi @09:49:18.75 Air.in :halfSpeed @09:49:18.84 Air.seek :fullVent @09:49:21.04 Puck Top Vented, bottom @5.7psi @09:49:21.05 Handoff.select :waste @09:49:21.18 delay 5 seconds @09:49:26.26 Handoff open to waste, bottom @-0.1psi @09:49:26.28 Air.in :default @09:49:26.37 Air.seek :evac @09:49:27.79 Lysis2.in :DAmix @09:49:27.91 Lysis1.in :DAmix @09:49:28.38 Lysis2.seek 1480uL @09:49:28.42 Lysis1.seek 575uL @09:50:03.60 delay 5 seconds @09:50:08.62 Handoff.select SPR @09:50:08.75 SPR.command "start 2 14" @09:50:09.79 SPR stabilized @09:50:09.93 delay 10 seconds @09:50:19.94 SPR.command "start 1 0" @09:50:22.66 Waiting up to 4:00 for SPR to enter state 1 @09:52:26.92 SPR.command "hold_on" @09:52:28.34 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @09:52:28.82 Lysis2.seek 1180uL @09:52:28.86 Lysis1.seek 275uL @09:53:10.95 delay 15 seconds @09:53:25.97 SPR.command "hold_off" @09:53:28.99 Waiting up to 38:00 for SPR to enter state 7 @10:11:28.58 SPRsummary:24RIU,160RIU,178RIU,244RIU,2.76ng/L,none,2.12ng/L after 3104s @10:18:05.80 SPR analysis completed @10:18:11.12 Ended log spr23apr2109h.spr.bz2 @10:18:11.14 SPR.command "stats_off" @10:18:13.02 -> Cmd.stop @10:18:13.12 Handoff.select :close @10:18:13.25 Gate.power :heat,:OFF @10:18:13.27 Gate.power :cartridge,:OFF @10:18:13.36 PinPort.in :unlocking @10:18:13.43 PinPort.seek :clear @10:18:13.91 Carousel.in :default @10:18:14.02 Carousel.home.seek 21 @10:18:14.32 Plunge.seek :locked @10:18:15.19 Archive.in :unlocking @10:18:15.33 Archive.seek :clear @10:18:15.37 Air.in :unlocking @10:18:15.54 Air.seek :clear @10:18:15.60 Lysis1.in :unlocking @10:18:15.76 Lysis1.seek :clear @10:18:15.85 Lysis2.in :unlocking @10:18:15.91 Lysis2.seek :clear @10:18:19.92 Gate.power :motor,:OFF @10:18:19.94 Gate.power :core,:OFF @10:18:19.96 Gate.power :analytic1,:OFF @10:18:19.99 Gate.power :analytic2,:OFF @10:18:20.01 Gate.power :analytic3,:OFF @10:18:20.04 Gate.power :main,:OFF @10:18:20.06 Safely stopped and ready to power off :STOPPED