@11:04:30.72PDT22-Apr-23
>BREAK< ESP3 Gateway v1.42 for core bus @11:04:30.77 >BREAK< ESP3 Gateway v1.42 for cartridge bus @11:04:33.38 Configuration for honu by brent@mbari.org 4/12/23 /home/esp/esp2/type/auv/honu/configure.rb @11:04:33.85 Sea pressure (Abs) tare is 6% of full scale @11:04:39.42 Polling Can every 2:00 @11:04:39.90 Clients may connect on TCP port 9999 Connecting to 10.89.5.2:9999 @11:04:39.94 Waiting for espclients... @11:04:40.06 Session Begun @11:04:42.00 -> showStatus true @11:04:44.02 -> Cmd.loadCartridge -10 @11:04:44.09 Gate.power :main,:ON,limit to 5000mA @11:04:44.11 Gate.power :analytic1,:ON,limit to 5000mA @11:04:44.13 Gate.power :analytic2,:ON,limit to 5000mA @11:04:44.16 Gate.power :analytic3,:ON,limit to 5000mA @11:04:44.29 Powering core bus @11:04:46.56 SPR Module 1, FW Rev. B, FPGA Rev. C @11:04:46.99 Archive.configure :default @11:04:47.08 Air.configure :default @11:04:47.15 Lysis1.configure :default @11:04:47.27 Lysis2.configure :default @11:04:47.36 Plunge.configure :default @11:04:47.45 SamplePump.configure :default @11:04:47.54 Toroid.configure :default @11:04:47.63 Carousel.configure :default @11:04:47.73 Pressure.configure :default @11:04:47.82 ExtraPump.configure :default @11:04:47.89 PinPort.configure :default @11:04:47.96 All elf controllers running firmware version 4.83 @11:04:47.99 Gate.power :motor,:ON,limit to 5000mA @11:04:48.29 Lysis1.home.jog 0 @11:04:48.53 Archive.home.jog 0 @11:04:48.64 Air.home.jog 0 @11:04:48.91 Carousel.home.jog 0 @11:04:48.95 Toroid.home.jog 0 @11:04:49.02 Lysis2.home.jog 0 @11:04:49.09 PinPort.home.jog 0 @11:04:49.13 Plunge.home.jog 0 @11:04:49.53 Air.in :homing @11:04:49.60 Archive.in :homing @11:04:49.64 Lysis1.in :homing @11:04:49.78 Air.jog 1383 @11:04:49.85 Lysis1.jog 157 @11:04:49.89 Archive.jog 1393 @11:04:50.10 Plunge.in :homing @11:04:50.12 PinPort.in :homing @11:04:50.15 Toroid.in :homing @11:04:50.19 Lysis2.in :homing @11:04:50.35 PinPort.jog -105 @11:04:50.40 Toroid.jog 620 @11:04:50.44 Lysis2.jog 1406 @11:04:50.49 Plunge.jog 1561 @11:04:50.68 Lysis1.setRawPosition! 50 @11:04:50.72 Lysis1.seek :home @11:04:50.80 PinPort.setRawPosition! 0 @11:04:50.85 PinPort.seek :home @11:04:50.94 PinPort.in :default @11:04:51.03 Air.setRawPosition! 50 @11:04:51.07 Lysis1.in :default @11:04:51.43 Air.seek :home @11:04:51.63 Archive.setRawPosition! 50 @11:04:51.68 Archive.seek :home @11:04:51.78 Lysis2.setRawPosition! 50 @11:04:51.83 Lysis2.seek :home @11:04:51.93 Air.in :default @11:04:51.96 Plunge.setRawPosition! 50 @11:04:52.05 Plunge.seek :home @11:04:52.16 Archive.in :default @11:04:52.19 Lysis2.in :default @11:04:52.34 Plunge.in :default @11:04:52.66 Toroid.setRawPosition! 0 @11:04:52.71 Toroid.seek :home @11:04:52.80 Toroid.in :default @11:04:52.88 Toroid.seek :clear @11:04:54.49 Carousel.in :default @11:04:54.62 Carousel.home.seek 10 @11:04:54.88 Selecting Cartridge 19 @11:04:55.15 Carousel.seek 19 @11:04:57.27 Lysis1.in :fast @11:04:57.34 Lysis1.seek :locked @11:04:59.94 Plunge.seek :locked @11:05:00.09 Air.in :fast @11:05:00.16 Air.seek :locked @11:05:01.73 Carousel.reconfigure :locked @11:05:01.87 Powering cartridge bus @11:05:04.51 Gate.setIR 1750,5000,0,6 @11:05:04.64 Gate.setIR 0,5000,0,6 @11:05:04.71 PuckHeater.configure :default @11:05:04.77 Gate.power :heat,:ON,limit to 5000mA @11:05:05.46 Mounted lysis Cartridge board#Q05075 @11:05:05.49 Using default cartridge heat loss curve for HeatingAccelInCarousel 19 @11:05:21.66 -> Cmd.startFiltering @11:05:21.91 Preparing to filter cartridge 19 92.07 @11:05:22.18 Priming sample loop w/100ml, bypass w/10ml @11:05:22.20 SamplePump.setPosition! 0ml @11:05:22.26 Bypass.open @11:05:23.81 Intake.open @11:05:24.53 Sea 7.9m deep (26.3psia) @11:05:24.55 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @11:05:27.13 Vacuum tare is 60% of full scale @11:05:27.26 Pressure.in :default @11:05:27.32 Pressure.hold @11:05:27.51 SamplePump.in :prime @11:05:27.59 SamplePump.seek 10ml,59 seconds @11:05:36.71 Pressure.stop @11:05:36.77 SamplePump.setPosition! 0ml @11:05:36.83 Exhaust.open @11:05:38.33 Bypass.close @11:05:39.88 Pressure.in :default @11:05:39.95 Pressure.hold @11:05:40.07 Sea 7.5m deep (25.8psia) @11:05:40.21 SamplePump.in :prime @11:05:40.29 SamplePump.seek 100ml,5:16 @11:07:17.67 Pressure.stop @11:07:17.75 Bypass.open @11:07:18.50 Intake.close @11:07:19.28 Toroid.in :hold @11:07:19.35 Toroid.seek :crack @11:07:20.88 Toroid.seek :cartridge @11:07:21.99 Toroid.in :default @11:07:22.06 Toroid.seek :clear @11:07:24.03 Pressurizing cartridge to 26psi above 26.1psia @11:07:24.04 SamplePump.setPosition! 0ml @11:07:24.10 Intake.open @11:07:24.77 Bypass.close @11:07:25.47 Exhaust.close @11:07:26.34 Pressure.in :default @11:07:26.41 Pressure.hold @11:07:26.60 SamplePump.in :charge @11:07:26.67 SamplePump.seek 2.3ml,42 seconds @11:07:37.08 Pressure.stop @11:07:37.14 Intake.close @11:07:37.90 Pressurized cartridge with 0.514ml to 26.0psi @11:07:37.91 Isolated @50.7psia (24.6psi) @11:07:37.94 Verifying cartridge holds at least 0.65..0.85 of 50.7psia @11:07:37.97 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @11:07:42.64 Sampling 1000.0ml in 1:25:20 @11:07:42.66 SamplePump.setPosition! 0ml @11:07:42.72 Exhaust.open @11:07:43.49 Intake.open @11:07:44.32 Pressure.in :default @11:07:44.38 Pressure.hold @11:07:44.50 Sea 8.6m deep (27.4psia) @11:07:44.51 Cannot pump 1.00ml/s -- Limiting to 0.86ml/s @11:07:44.67 SamplePump.in :sample @11:07:44.74 SamplePump.seek 1000ml,1:23:51 @11:07:47.73 Cartridge 19 filtering 1/1000ml at 0.42ml/s with 23.7psi vacuum 7.0m deep (25.0psia) @11:08:47.36 Cartridge 19 filtering 25/1000ml at 0.39ml/s with 23.6psi vacuum 5.8m deep (23.3psia) @11:09:47.36 Cartridge 19 filtering 49/1000ml at 0.38ml/s with 24.1psi vacuum 5.5m deep (22.8psia) @11:10:47.36 Cartridge 19 filtering 73/1000ml at 0.38ml/s with 27.1psi vacuum 5.3m deep (22.5psia) @11:11:47.36 Cartridge 19 filtering 96/1000ml at 0.37ml/s with 27.0psi vacuum 5.2m deep (22.4psia) @11:12:47.36 Cartridge 19 filtering 118/1000ml at 0.39ml/s with 23.9psi vacuum 7.1m deep (25.2psia) @11:13:47.35 Cartridge 19 filtering 140/1000ml at 0.36ml/s with 26.8psi vacuum 5.1m deep (22.2psia) @11:14:47.36 Cartridge 19 filtering 162/1000ml at 0.34ml/s with 24.5psi vacuum 6.5m deep (24.3psia) @11:15:47.35 Cartridge 19 filtering 183/1000ml at 0.35ml/s with 22.7psi vacuum 5.9m deep (23.4psia) @11:16:47.36 Cartridge 19 filtering 204/1000ml at 0.33ml/s with 23.5psi vacuum 6.1m deep (23.7psia) @11:17:47.36 Cartridge 19 filtering 225/1000ml at 0.33ml/s with 24.1psi vacuum 6.7m deep (24.6psia) @11:18:47.35 Cartridge 19 filtering 245/1000ml at 0.35ml/s with 24.1psi vacuum 6.2m deep (23.9psia) @11:19:47.36 Cartridge 19 filtering 265/1000ml at 0.34ml/s with 22.3psi vacuum 6.6m deep (24.5psia) @11:20:47.36 Cartridge 19 filtering 285/1000ml at 0.33ml/s with 23.1psi vacuum 6.0m deep (23.5psia) @11:21:47.36 Cartridge 19 filtering 305/1000ml at 0.33ml/s with 22.6psi vacuum 7.2m deep (25.3psia) @11:22:47.36 Cartridge 19 filtering 324/1000ml at 0.33ml/s with 23.0psi vacuum 6.8m deep (24.7psia) @11:23:47.39 Cartridge 19 filtering 343/1000ml at 0.32ml/s with 24.1psi vacuum 8.3m deep (26.9psia) @11:24:47.36 Cartridge 19 filtering 361/1000ml at 0.30ml/s with 24.9psi vacuum 7.1m deep (25.2psia) @11:25:47.36 Cartridge 19 filtering 379/1000ml at 0.29ml/s with 23.1psi vacuum 6.5m deep (24.4psia) @11:26:47.36 Cartridge 19 filtering 397/1000ml at 0.31ml/s with 25.1psi vacuum 5.4m deep (22.6psia) @11:27:47.36 Cartridge 19 filtering 415/1000ml at 0.27ml/s with 25.0psi vacuum 5.8m deep (23.3psia) @11:28:47.35 Cartridge 19 filtering 432/1000ml at 0.29ml/s with 23.5psi vacuum 5.8m deep (23.2psia) @11:29:47.36 Cartridge 19 filtering 449/1000ml at 0.27ml/s with 23.3psi vacuum 6.7m deep (24.6psia) @11:30:47.36 Cartridge 19 filtering 466/1000ml at 0.26ml/s with 24.2psi vacuum 5.8m deep (23.3psia) @11:31:47.36 Cartridge 19 filtering 482/1000ml at 0.26ml/s with 21.1psi vacuum 7.6m deep (25.9psia) @11:32:47.41 Cartridge 19 filtering 499/1000ml at 0.29ml/s with 23.5psi vacuum 7.3m deep (25.5psia) @11:33:47.35 Cartridge 19 filtering 515/1000ml at 0.28ml/s with 23.7psi vacuum 5.4m deep (22.7psia) @11:34:47.36 Cartridge 19 filtering 531/1000ml at 0.27ml/s with 23.6psi vacuum 6.6m deep (24.4psia) @11:35:47.36 Cartridge 19 filtering 547/1000ml at 0.25ml/s with 24.2psi vacuum 5.6m deep (22.9psia) @11:36:47.36 Cartridge 19 filtering 562/1000ml at 0.26ml/s with 24.2psi vacuum 6.5m deep (24.3psia) @11:37:47.36 Cartridge 19 filtering 578/1000ml at 0.25ml/s with 22.9psi vacuum 7.8m deep (26.2psia) @11:38:47.36 Cartridge 19 filtering 593/1000ml at 0.24ml/s with 23.8psi vacuum 5.4m deep (22.7psia) @11:39:47.36 Cartridge 19 filtering 608/1000ml at 0.26ml/s with 25.2psi vacuum 6.7m deep (24.5psia) @11:40:47.36 Cartridge 19 filtering 623/1000ml at 0.23ml/s with 23.6psi vacuum 6.1m deep (23.6psia) @11:41:47.36 Cartridge 19 filtering 638/1000ml at 0.25ml/s with 26.1psi vacuum 6.4m deep (24.2psia) @11:42:47.36 Cartridge 19 filtering 652/1000ml at 0.24ml/s with 23.6psi vacuum 5.9m deep (23.4psia) @11:43:47.36 Cartridge 19 filtering 667/1000ml at 0.23ml/s with 25.3psi vacuum 6.2m deep (23.8psia) @11:44:47.67 Cartridge 19 filtering 681/1000ml at 0.23ml/s with 23.6psi vacuum 6.3m deep (23.9psia) @11:45:47.36 Cartridge 19 filtering 695/1000ml at 0.23ml/s with 23.8psi vacuum 5.8m deep (23.2psia) @11:46:47.36 Cartridge 19 filtering 709/1000ml at 0.23ml/s with 25.8psi vacuum 6.7m deep (24.7psia) @11:47:47.36 Cartridge 19 filtering 724/1000ml at 0.22ml/s with 23.2psi vacuum 5.7m deep (23.1psia) @11:48:47.36 Cartridge 19 filtering 737/1000ml at 0.22ml/s with 22.7psi vacuum 6.2m deep (23.9psia) @11:49:47.36 Cartridge 19 filtering 751/1000ml at 0.23ml/s with 24.3psi vacuum 7.3m deep (25.5psia) @11:50:47.36 Cartridge 19 filtering 765/1000ml at 0.22ml/s with 24.7psi vacuum 7.2m deep (25.4psia) @11:51:47.36 Cartridge 19 filtering 778/1000ml at 0.24ml/s with 25.6psi vacuum 5.5m deep (22.8psia) @11:52:47.36 Cartridge 19 filtering 792/1000ml at 0.21ml/s with 23.0psi vacuum 6.7m deep (24.5psia) @11:53:47.35 Cartridge 19 filtering 805/1000ml at 0.20ml/s with 25.0psi vacuum 5.5m deep (22.8psia) @11:54:47.36 Cartridge 19 filtering 818/1000ml at 0.22ml/s with 25.4psi vacuum 6.0m deep (23.5psia) @11:55:47.36 Cartridge 19 filtering 831/1000ml at 0.20ml/s with 24.6psi vacuum 5.7m deep (23.1psia) @11:56:47.36 Cartridge 19 filtering 844/1000ml at 0.21ml/s with 23.7psi vacuum 6.1m deep (23.7psia) @11:57:47.36 Cartridge 19 filtering 857/1000ml at 0.22ml/s with 24.4psi vacuum 6.3m deep (24.0psia) @11:58:47.36 Cartridge 19 filtering 870/1000ml at 0.20ml/s with 24.2psi vacuum 6.0m deep (23.5psia) @11:59:47.36 Cartridge 19 filtering 882/1000ml at 0.20ml/s with 25.0psi vacuum 6.0m deep (23.6psia) @12:00:47.36 Cartridge 19 filtering 895/1000ml at 0.21ml/s with 24.1psi vacuum 5.8m deep (23.3psia) @12:01:47.36 Cartridge 19 filtering 907/1000ml at 0.20ml/s with 25.0psi vacuum 6.5m deep (24.3psia) @12:02:47.36 Cartridge 19 filtering 920/1000ml at 0.20ml/s with 25.6psi vacuum 5.3m deep (22.6psia) @12:03:47.36 Cartridge 19 filtering 932/1000ml at 0.21ml/s with 24.8psi vacuum 6.6m deep (24.5psia) @12:04:47.36 Cartridge 19 filtering 944/1000ml at 0.21ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @12:05:47.36 Cartridge 19 filtering 956/1000ml at 0.20ml/s with 24.0psi vacuum 5.6m deep (23.0psia) @12:06:47.36 Cartridge 19 filtering 968/1000ml at 0.20ml/s with 25.7psi vacuum 6.3m deep (24.0psia) @12:07:47.36 Cartridge 19 filtering 981/1000ml at 0.20ml/s with 24.1psi vacuum 5.9m deep (23.4psia) @12:08:47.36 Cartridge 19 filtering 993/1000ml at 0.20ml/s with 25.3psi vacuum 6.3m deep (24.0psia) @12:09:23.94 Pressure.stop @12:09:24.06 Intake.close @12:09:24.13 Exhaust.close @12:09:24.31 Toroid.in :hold @12:09:24.38 Toroid.seek :bypass @12:09:26.00 Toroid.in :default @12:09:26.07 Toroid.seek :clear @12:09:27.68 Sampled 1000.0ml @12:09:29.15 -> Cmd.startProcessing 120.0 @12:09:30.60 SPR.command "read_sequences" @12:09:30.97 SPR.command "stats_on" @12:09:31.10 SPR.command "start 0 1" @12:09:31.82 SPR.start /var/log/esp/spr23apr2212h.spr.bz2 @12:09:34.31 Handoff.select :waste @12:09:41.00 Syringe tare is 17% of full scale @12:09:41.02 PinPort.in :locking @12:09:41.10 PinPort.seek :locked @12:09:41.58 Archive.in :fast @12:09:41.63 Lysis2.in :fast @12:09:41.71 Lysis1.seek 1350uL @12:09:41.79 Archive.seek :full @12:09:41.88 Lysis2.seek :full @12:09:43.89 Plunge.seek :reagent @12:09:44.81 Evacuating puck with filter bubble point of 50psi @12:09:44.83 PinPort.seek :locked @12:09:44.93 Handoff.select :waste @12:09:51.53 Air syringe pumping up to 1 strokes to reach 8psi @12:09:51.54 Air stroke #1 of 1 @12:09:51.64 Air.in :pressurize @12:09:51.70 Air.seek :pressurized @12:09:57.44 0.1psi after pressurize @12:09:57.46 Air.in :compress @12:09:57.54 Air.seek :evac @12:10:00.54 0.8psi after compress @12:10:11.12 0.2psi after compress (stable) @12:10:11.14 Air.in :default @12:10:11.24 Air.hold @12:10:11.43 at 0.1psi Opened to waste @12:10:11.44 Handoff.select :blocked @12:10:11.58 Archive syringe pumping up to 8 strokes to reach 50psi @12:10:11.59 Archive stroke #1 of 8 @12:10:11.68 Archive.in :pressurize @12:10:11.76 Archive.seek :pressurized @12:10:24.32 9.6psi after pressurize @12:10:51.39 23.6psi after pressurize (stable) @12:10:51.41 Archive.in :compress @12:10:51.49 Archive.seek :empty @12:11:06.12 34.9psi after compress @12:11:18.19 37.5psi after compress (stable) @12:11:18.21 Archive.in :gulp @12:11:18.31 Archive.seek :topVent @12:11:24.58 37.6psi after gulp @12:11:30.16 37.6psi after gulp (stable) @12:11:30.17 Archive stroke #2 of 8 @12:11:30.27 Archive.in :pressurize @12:11:30.34 Archive.seek :pressurized @12:11:42.84 35.5psi after pressurize @12:11:56.42 41.3psi after pressurize (stable) @12:11:56.44 Archive.in :compress @12:11:56.52 Archive.seek :empty @12:12:05.38 Reached 50.1 of 50.0psi after pumping 3335uL (1.93 strokes) @12:12:05.39 Archive.in :default @12:12:05.48 Archive.hold @12:12:05.55 Handoff.select :waste @12:12:14.80 delay 1:00 @12:13:14.82 Handoff.select :blocked @12:13:37.52 Handoff.select :waste @12:13:37.64 0.8psi at evacEnd @12:13:37.65 Handoff.select :blocked @12:13:37.79 Archive.in :fast @12:13:37.85 Archive.seek :topVent @12:13:51.14 Stable @1.5psi after Handoff blocked @12:13:51.17 Archive syringe pumping up to 8 strokes to reach 31psi @12:13:51.18 Archive stroke #1 of 8 @12:13:51.29 Archive.in :pressurize @12:13:51.36 Archive.seek :pressurized @12:14:03.90 2.2psi after pressurize @12:14:03.92 Archive.in :compress @12:14:04.00 Archive.seek :empty @12:14:18.63 10.8psi after compress @12:14:54.21 27.5psi after compress (stable) @12:14:54.23 Archive.in :gulp @12:14:54.31 Archive.seek :topVent @12:15:00.55 28.6psi after gulp @12:15:06.15 29.1psi after gulp (stable) @12:15:06.16 Archive stroke #2 of 8 @12:15:06.28 Archive.in :pressurize @12:15:06.37 Archive.seek :pressurized @12:15:18.87 29.7psi after pressurize @12:15:18.89 Archive.in :compress @12:15:18.97 Archive.seek :empty @12:15:28.70 Reached 31.5 of 31.5psi after pumping 3354uL (1.94 strokes) @12:15:28.72 Archive.in :default @12:15:28.80 Archive.hold @12:15:28.87 Handoff.select :waste @12:15:42.06 delay 1:00 @12:16:42.07 Handoff.select :blocked @12:17:04.76 Handoff.select :waste @12:17:04.88 2.6psi at evacEnd @12:17:04.90 Air.seek :fullVent @12:17:06.33 Archive.in :fast @12:17:06.42 Archive.seek :topVent @12:17:13.17 Seawater evacuated, Pressure above/below filter released. @12:17:13.26 Lysis1.in :slow @12:17:13.33 Lysis1.seek 1050uL @12:17:43.39 delay 5 seconds @12:17:48.41 Handoff.select :blocked @12:17:48.60 Lysis1.seek 450uL @12:18:48.63 delay 3 seconds @12:18:51.71 Air.in mix with 1.40048 parts Lysis1 @12:18:52.18 Air.seek :evac @12:18:52.25 Lysis1.seek 300uL @12:19:07.38 Lysis1.seek :empty @12:19:37.52 Lysis buffer delivered @22.6psi @12:19:37.53 Polling Can every 2:00 @12:19:38.08 PuckHeater.configure :default #ambient 11.9C @12:19:38.13 PuckHeater.seek 80.2C @12:20:51.04 PuckHeater.hold 2:00,80.2C @12:22:50.51 Polling Can every 2:00 @12:22:51.05 PuckHeater.configure :default #ambient 12.0C @12:22:51.10 PuckHeater.hold 30 seconds,79.2C @12:23:21.15 Polling Can every 2:00 @12:23:21.69 PuckHeater.configure :default #ambient 12.0C @12:23:21.75 PuckHeater.hold 30 seconds,78.6C @12:23:51.80 Polling Can every 2:00 @12:23:52.34 PuckHeater.configure :default #ambient 11.9C @12:23:52.40 PuckHeater.hold 10:00,78.1C @12:33:49.57 Lysis complete. Syringe pressure @21.4psi @12:33:49.59 Archive syringe pumping up to 5 strokes to reach 40psi @12:33:49.61 Archive stroke #1 of 5 @12:33:49.71 Archive.in :pressurize @12:33:49.78 Archive.seek :pressurized @12:34:02.31 21.5psi after pressurize @12:34:11.38 38.9psi after pressurize (stable) @12:34:11.40 Archive.in :compress @12:34:11.48 Archive.seek :empty @12:34:13.17 Reached 40.5 of 40.0psi after pumping 1451uL (0.84 strokes) @12:34:13.18 Archive.in :default @12:34:13.26 Archive.hold @12:34:13.33 delay 2 seconds @12:34:15.35 Archive.in :fast @12:34:15.42 Archive.seek :full @12:34:21.65 Lysis1.seek 138uL @12:34:35.56 delay 1 second @12:34:36.58 Archive.seek :full @12:34:36.62 Lysis1.seek :empty @12:34:50.90 Handoff.select :blocked @12:34:51.06 Initial air pressure @40.4psi @12:34:51.12 Lysis1.in :extract @12:34:51.21 Lysis1.seek 600uL,nil @12:34:54.15 Archive syringe pumping up to 6 strokes to reach 25psi @12:34:54.16 Archive stroke #1 of 6 @12:34:54.29 Archive.in :pressurize @12:34:54.36 Archive.seek :pressurized @12:35:06.90 15.4psi after pressurize @12:35:11.09 Archive.in :default @12:35:11.15 Archive.hold @12:35:11.30 Initial 600uL lysate @15.5psi @12:35:11.32 Handoff.select :blocked @12:35:11.43 Initial air pressure @16.2psi @12:35:11.51 Lysis1.seek 825uL,nil @12:35:12.71 Archive syringe pumping up to 6 strokes to reach 17psi @12:35:12.72 Archive stroke #1 of 6 @12:35:12.82 Archive.in :compress @12:35:12.90 Archive.seek :empty @12:35:24.86 Archive.in :default @12:35:24.92 Archive.hold @12:35:25.15 Recovered 825uL lysate @13.9psi @12:35:25.17 Air.in :halfSpeed @12:35:25.25 Air.seek :fullVent @12:35:27.45 Puck Top Vented, bottom @3.1psi @12:35:27.47 Handoff.select :waste @12:35:27.61 delay 5 seconds @12:35:32.67 Handoff open to waste, bottom @-0.1psi @12:35:32.69 Air.in :default @12:35:32.77 Air.seek :evac @12:35:34.19 Lysis2.in :DAmix @12:35:34.31 Lysis1.in :DAmix @12:35:34.75 Lysis2.seek 1480uL @12:35:34.81 Lysis1.seek 575uL @12:36:09.95 delay 5 seconds @12:36:14.97 Handoff.select SPR @12:36:15.09 SPR.command "start 2 14" @12:36:16.06 SPR stabilized @12:36:16.20 delay 10 seconds @12:36:26.22 SPR.command "start 1 0" @12:36:29.43 Waiting up to 4:00 for SPR to enter state 1 @12:38:33.69 SPR.command "hold_on" @12:38:35.59 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @12:38:36.05 Lysis2.seek 1180uL @12:38:36.11 Lysis1.seek 275uL @12:39:18.17 delay 15 seconds @12:39:33.18 SPR.command "hold_off" @12:39:35.76 Waiting up to 38:00 for SPR to enter state 7 @12:57:34.39 SPRsummary:28RIU,105RIU,136RIU,165RIU,11ng/L,4.78ng/L,9.57ng/L after 3045s @13:04:11.61 SPR analysis completed @13:04:16.44 Ended log spr23apr2212h.spr.bz2 @13:04:16.45 SPR.command "stats_off" @13:04:17.81 -> Cmd.stop @13:04:17.91 Handoff.select :close @13:04:18.04 Gate.power :heat,:OFF @13:04:18.06 Gate.power :cartridge,:OFF @13:04:18.15 PinPort.in :unlocking @13:04:18.22 PinPort.seek :clear @13:04:18.70 Carousel.in :default @13:04:18.82 Carousel.home.seek 19 @13:04:19.13 Plunge.seek :locked @13:04:20.00 Archive.in :unlocking @13:04:20.14 Archive.seek :clear @13:04:20.18 Air.in :unlocking @13:04:20.37 Lysis1.in :unlocking @13:04:20.41 Air.seek :clear @13:04:20.59 Lysis1.seek :clear @13:04:20.68 Lysis2.in :unlocking @13:04:20.75 Lysis2.seek :clear @13:04:24.70 Gate.power :motor,:OFF @13:04:24.72 Gate.power :core,:OFF @13:04:24.75 Gate.power :analytic1,:OFF @13:04:24.77 Gate.power :analytic2,:OFF @13:04:24.79 Gate.power :analytic3,:OFF @13:04:24.82 Gate.power :main,:OFF @13:04:24.84 Safely stopped and ready to power off :STOPPED