@15:55:42.84PDT13-May-22
>BREAK< ESP3 Gateway v1.12 for core bus @15:55:42.86 >BREAK< ESP3 Gateway v1.12 for cartridge bus @15:55:45.42 Configuration for honu by methane@mbari.org 3/16/22 /home/esp/esp2/type/auv/honu/configure.rb @15:55:51.69 Polling Can every 2:00 @15:55:52.18 Clients may connect on TCP port 9999 @15:55:52.19 Connecting to 10.89.5.2:9999 @15:55:52.23 Waiting for espclients... @15:55:52.52 Session Begun @15:55:54.84 -> showStatus true @15:55:56.46 -> Cmd.loadCartridge -10 @15:55:56.52 Gate.power :main,:ON,limit to 5000mA @15:55:56.54 Gate.power :analytic1,:ON,limit to 5000mA @15:55:56.56 Gate.power :analytic2,:ON,limit to 5000mA @15:55:56.59 Gate.power :analytic3,:ON,limit to 5000mA @15:55:56.73 Powering core bus @15:55:58.99 SPR Module 1, FW Rev. B, FPGA Rev. C @15:55:59.44 Archive.configure :default @15:55:59.57 Air.configure :default @15:55:59.68 Lysis1.configure :default @15:55:59.78 Lysis2.configure :default @15:55:59.92 Plunge.configure :default @15:56:00.06 SamplePump.configure :default @15:56:00.19 Toroid.configure :default @15:56:00.32 Carousel.configure :default @15:56:00.44 Pressure.configure :default @15:56:00.57 ExtraPump.configure :default @15:56:00.68 PinPort.configure :default @15:56:00.74 All elf controllers running firmware version 4.79 @15:56:00.77 Gate.power :motor,:ON,limit to 5000mA @15:56:00.94 Archive.in :default @15:56:01.25 Archive.home.jog 0 @15:56:01.44 Toroid.in :default @15:56:01.49 Plunge.in :default @15:56:01.53 Lysis1.in :default @15:56:01.59 Air.in :default @15:56:01.64 PinPort.in :default @15:56:01.69 Lysis2.in :default @15:56:01.88 Toroid.home.jog 0 @15:56:01.91 Plunge.home.jog 0 @15:56:01.94 Lysis1.home.jog 0 @15:56:01.97 Air.home.jog 0 @15:56:02.00 PinPort.home.jog 0 @15:56:02.06 Lysis2.home.jog 0 @15:56:02.52 Archive.in :homing @15:56:02.73 Archive.jog 1395 @15:56:02.81 PinPort.in :homing @15:56:02.86 Plunge.in :homing @15:56:02.89 Toroid.in :homing @15:56:02.93 Air.in :homing @15:56:02.97 Lysis1.in :homing @15:56:03.03 Lysis2.in :homing @15:56:03.22 PinPort.jog -105 @15:56:03.27 Plunge.jog 113 @15:56:03.30 Toroid.jog 635 @15:56:03.33 Air.jog 1383 @15:56:03.36 Lysis2.jog 1405 @15:56:03.39 Lysis1.jog 1376 @15:56:03.65 PinPort.setRawPosition! 0 @15:56:03.70 PinPort.seek :home @15:56:03.78 PinPort.in :default @15:56:03.87 Plunge.setRawPosition! 50 @15:56:03.92 Plunge.seek :home @15:56:04.15 Archive.setRawPosition! 50 @15:56:04.20 Archive.seek :home @15:56:04.28 Plunge.in :default @15:56:04.48 Archive.in :default @15:56:04.64 Lysis2.setRawPosition! 50 @15:56:04.71 Lysis2.seek :home @15:56:04.80 Air.setRawPosition! 50 @15:56:04.82 Lysis1.setRawPosition! 50 @15:56:04.89 Air.seek :home @15:56:04.94 Lysis1.seek :home @15:56:05.08 Lysis2.in :default @15:56:05.24 Air.in :default @15:56:05.33 Lysis1.in :default @15:56:05.62 Toroid.setRawPosition! 0 @15:56:05.67 Toroid.seek :home @15:56:05.76 Toroid.in :default @15:56:05.89 Toroid.seek :clear @15:56:07.51 Carousel.in :default @15:56:07.57 Carousel.home.jog 0 @15:56:08.05 Carousel.in :default @15:56:08.11 Carousel.seek 11 @15:56:08.27 Selecting Cartridge 60 @15:56:08.53 Carousel.seek 60 @15:56:18.81 Lysis1.in :fast @15:56:18.87 Lysis1.seek :locked @15:56:21.48 Plunge.seek :locked @15:56:21.61 Air.in :fast @15:56:21.67 Air.seek :locked @15:56:24.13 Powering cartridge bus @15:56:26.74 Gate.setIR 1750,5000,0,6 @15:56:26.86 Gate.setIR 0,5000,0,6 @15:56:26.92 PuckHeater.configure :default @15:56:26.98 Gate.power :heat,:ON,limit to 5000mA @15:56:27.68 Mounted lysis Cartridge base#L5-0018, top#L8-0070, board#Q05043 @15:56:27.72 Using default puck heat loss curve for HeatingAccelInCarousel 60 @15:56:36.86 -> Cmd.startFiltering 17.07@15:56:37.11 Preparing to filter cartridge 60 17.07@15:56:37.11 Preparing to filter cartridge 60 @15:56:37.37 Priming sample loop w/100ml, bypass w/10ml @15:56:37.38 SamplePump.setPosition! 0ml @15:56:37.43 Bypass.open @15:56:38.87 Intake.open @15:56:39.59 Sea 2.7m deep (18.7psia) @15:56:39.60 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @15:56:42.17 Vacuum tare is 54% of full scale @15:56:42.30 Pressure.in :default @15:56:42.36 Pressure.hold @15:56:42.54 SamplePump.in :prime @15:56:42.61 SamplePump.seek 10ml,59 seconds @15:56:51.22 Pressure.stop @15:56:51.27 SamplePump.setPosition! 0ml @15:56:51.31 Exhaust.open @15:56:52.73 Bypass.close @15:56:54.20 Pressure.in :default @15:56:54.26 Pressure.hold @15:56:54.38 Sea 2.7m deep (18.7psia) @15:56:54.52 SamplePump.in :prime @15:56:54.58 SamplePump.seek 100ml,5:16 @15:58:18.76 Pressure.stop @15:58:18.82 Bypass.open @15:58:19.49 Intake.close @15:58:20.24 Toroid.in :hold @15:58:20.30 Toroid.seek :crack @15:58:21.81 Toroid.seek :cartridge @15:58:22.91 Toroid.in :default @15:58:22.97 Toroid.seek :clear @15:58:25.32 Pressurizing cartridge to 26psi above 18.8psia @15:58:25.34 SamplePump.setPosition! 0ml @15:58:25.39 Intake.open @15:58:26.06 Bypass.close @15:58:26.73 Exhaust.close @15:58:27.53 Pressure.in :default @15:58:27.59 Pressure.hold @15:58:27.77 SamplePump.in :charge @15:58:27.84 SamplePump.seek 2.3ml,42 seconds @15:58:41.79 Pressure.stop @15:58:41.84 Intake.close @15:58:42.56 Pressurized cartridge with 0.941ml to 26.3psi @15:58:42.58 Isolated @42.5psia (23.7psi) @15:58:42.60 Verifying cartridge holds at least 0.65..0.85 of 42.5psia @15:58:42.63 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @15:58:47.26 Sampling 100.0ml @15:58:47.28 SamplePump.setPosition! 0ml @15:58:47.34 Exhaust.open @15:58:48.09 Intake.open @15:58:48.88 Pressure.in :default @15:58:48.94 Pressure.hold @15:58:49.05 Sea 3.3m deep (19.6psia) @15:58:49.19 SamplePump.in :sample @15:58:49.27 SamplePump.seek 100ml,8:50 @15:58:51.90 Cartridge 60 filtering 1/100ml at 0.51ml/s with 19.6psi vacuum 5.6m deep (22.9psia) @15:59:51.90 Cartridge 60 filtering 30/100ml at 0.42ml/s with 28.6psi vacuum @16:00:51.91 Cartridge 60 filtering 54/100ml at 0.32ml/s with 28.9psi vacuum @16:01:51.90 Cartridge 60 filtering 72/100ml at 0.24ml/s with 21.8psi vacuum @16:02:51.90 Cartridge 60 filtering 86/100ml at 0.22ml/s with 23.6psi vacuum 2.1m deep (17.9psia) @16:02:54.29 Flow averaged 0.35ml/s over 4:06 @16:02:54.32 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @16:02:54.90 Relieved excess vacuum in .6 seconds @16:02:54.92 SamplePump.seek 100ml,1:40 @16:03:00.15 Flow estimate reduced to 0.28ml/s @16:03:00.19 Waiting up to 10 seconds for vacuum to reduce from 27.0 to 18.0psi @16:03:00.76 Relieved excess vacuum in .6 seconds @16:03:00.79 SamplePump.seek 100ml,1:34 @16:03:04.07 Flow estimate reduced to 0.22ml/s @16:03:04.11 Waiting up to 10 seconds for vacuum to reduce from 26.9 to 18.0psi @16:03:04.68 Relieved excess vacuum in .6 seconds @16:03:04.70 SamplePump.seek 100ml,1:31 @16:03:09.25 Flow estimate reduced to 0.18ml/s @16:03:09.27 Intake.close @16:03:09.34 Exhaust.close @16:03:09.51 Toroid.in :hold @16:03:09.58 Toroid.seek :bypass @16:03:11.20 Toroid.in :default @16:03:11.27 Toroid.seek :clear @16:03:12.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:03:12.90 Pressure.stop @16:03:13.09 Sampled 88.8ml @16:03:15.25 -> Cmd.startProcessing 120.0 @16:03:16.68 SPR.command "read_sequences" @16:03:16.78 SPR.command "stats_on" @16:03:16.88 SPR.command "start 0 1" @16:03:17.63 SPR.start /var/log/esp/spr22may1316h.spr.bz2 @16:03:19.63 Handoff.select :waste @16:03:26.31 Syringe tare is 17% of full scale @16:03:26.33 PinPort.in :locking @16:03:26.39 PinPort.seek :locked @16:03:26.88 Archive.in :fast @16:03:26.96 Lysis2.in :fast @16:03:27.04 Archive.seek :full @16:03:27.09 Lysis1.seek 1350uL @16:03:27.15 Lysis2.seek :full @16:03:29.14 Plunge.seek :reagent @16:03:30.01 Evacuating puck with filter bubble point of 50psi @16:03:30.03 PinPort.seek :locked @16:03:30.12 Handoff.select :waste @16:03:36.70 Air syringe pumping up to 1 stroke to reach 8psi @16:03:36.71 Air stroke #1 of 1 @16:03:36.80 Air.in :pressurize @16:03:36.86 Air.seek :pressurized @16:03:48.44 0.9psi after pressurize @16:03:48.48 Air.in :compress @16:03:48.58 Air.seek :evac @16:03:52.66 0.8psi after compress @16:04:05.23 0.1psi after compress (stable) @16:04:05.25 Air.in :default @16:04:05.31 Air.hold @16:04:05.52 at 0.1psi Opened to waste @16:04:05.54 Handoff.select :blocked @16:04:05.68 Archive syringe pumping up to 8 strokes to reach 50psi @16:04:05.69 Archive stroke #1 of 8 @16:04:05.78 Archive.in :pressurize @16:04:05.84 Archive.seek :pressurized @16:04:18.72 9.5psi after pressurize @16:04:18.75 Archive.in :compress @16:04:18.83 Archive.seek :empty @16:04:33.51 30.7psi after compress @16:04:46.08 32.9psi after compress (stable) @16:04:46.10 Archive.in :gulp @16:04:46.22 Archive.seek :topVent @16:04:53.38 33.3psi after gulp @16:05:05.95 33.1psi after gulp (stable) @16:05:05.96 Archive stroke #2 of 8 @16:05:06.07 Archive.in :pressurize @16:05:06.14 Archive.seek :pressurized @16:05:18.98 32.9psi after pressurize @16:05:19.01 Archive.in :compress @16:05:19.08 Archive.seek :empty @16:05:32.03 Reached 50.2 of 50.0psi after pumping 3423uL (1.98 strokes) @16:05:32.05 Archive.in :default @16:05:32.12 Archive.hold @16:05:32.18 Handoff.select :waste @16:05:39.74 delay 1:00 @16:06:39.75 Handoff.select :blocked @16:07:02.43 Handoff.select :waste @16:07:02.55 2.4psi at evacEnd @16:07:02.56 Handoff.select :blocked @16:07:02.69 Archive.in :fast @16:07:02.76 Archive.seek :topVent @16:07:16.30 Stable @2.9psi after Handoff blocked @16:07:16.31 Archive syringe pumping up to 8 strokes to reach 33psi @16:07:16.33 Archive stroke #1 of 8 @16:07:16.43 Archive.in :pressurize @16:07:16.50 Archive.seek :pressurized @16:07:29.38 4.0psi after pressurize @16:07:29.40 Archive.in :compress @16:07:29.47 Archive.seek :empty @16:07:44.16 12.1psi after compress @16:08:15.73 28.8psi after compress (stable) @16:08:15.75 Archive.in :gulp @16:08:15.82 Archive.seek :topVent @16:08:22.98 29.8psi after gulp @16:08:35.54 30.5psi after gulp (stable) @16:08:35.56 Archive stroke #2 of 8 @16:08:35.65 Archive.in :pressurize @16:08:35.71 Archive.seek :pressurized @16:08:48.57 31.2psi after pressurize @16:08:48.59 Archive.in :compress @16:08:48.66 Archive.seek :empty @16:09:00.61 Reached 33.0 of 32.9psi after pumping 3401uL (1.97 strokes) @16:09:00.65 Archive.in :default @16:09:00.71 Archive.hold @16:09:00.78 Handoff.select :waste @16:09:15.52 delay 1:00 @16:10:15.54 Handoff.select :blocked @16:10:38.22 Handoff.select :waste @16:10:38.34 1.2psi at evacEnd @16:10:38.36 Air.seek :fullVent @16:10:39.78 Archive.in :fast @16:10:39.85 Archive.seek :topVent @16:10:46.74 Seawater evacuated, Presure above/below filter released. Lysis begins @16:10:46.83 Lysis1.in :slow @16:10:46.90 Lysis1.seek 1050uL @16:11:16.93 delay 5 seconds @16:11:21.94 Handoff.select :blocked @16:11:22.12 Lysis1.seek 450uL @16:12:22.10 delay 3 seconds @16:12:25.17 Air.in mix with 1.40048 parts Lysis1 @16:12:25.59 Air.seek :evac @16:12:25.63 Lysis1.seek 300uL @16:12:40.74 Lysis1.seek :empty @16:13:10.83 Lysis buffer delivered @30.1psi @16:13:10.84 Polling Can every 2:00 @16:13:11.39 PuckHeater.configure :default #ambient 20.2C @16:13:11.44 PuckHeater.seek 79.8C @16:14:04.98 PuckHeater.hold 2:00,79.8C @16:16:04.88 Polling Can every 2:00 @16:16:05.42 PuckHeater.configure :default #ambient 20.3C @16:16:05.47 PuckHeater.hold 30 seconds,78.8C @16:16:35.63 Polling Can every 2:00 @16:16:36.17 PuckHeater.configure :default #ambient 20.3C @16:16:36.22 PuckHeater.hold 30 seconds,78.2C @16:17:06.37 Polling Can every 2:00 @16:17:06.91 PuckHeater.hold 10:00,77.7C @16:27:06.26 Lysis complete. Syringe pressure @41.0psi @16:27:06.27 Handoff.select :blocked @16:27:06.32 Archive syringe pumping up to 5 strokes to reach 40psi @16:27:06.33 Archive stroke #1 of 5 @16:27:06.44 Archive.in :pressurize @16:27:06.52 Archive.seek :pressurized @16:27:06.69 Reached 41.0 of 40.0psi after pumping 0uL (0.00 strokes) @16:27:06.71 Archive.in :default @16:27:06.78 Archive.hold @16:27:06.85 delay 2 seconds @16:27:08.87 Archive.in :fast @16:27:08.93 Archive.seek :full @16:27:09.11 Lysis1.seek 138uL @16:27:22.98 delay 1 second @16:27:24.00 Archive.seek :full @16:27:24.04 Lysis1.seek :empty @16:27:38.02 Initial air pressure @40.6psi @16:27:38.10 Lysis1.in :extract @16:27:38.18 Lysis1.seek 600uL,nil @16:27:39.85 Archive syringe pumping up to 6 strokes to reach 25psi @16:27:39.86 Archive stroke #1 of 6 @16:27:39.95 Archive.in :pressurize @16:27:40.04 Archive.seek :pressurized @16:27:52.90 16.1psi after pressurize @16:27:52.91 Archive.in :compress @16:27:52.99 Archive.seek :empty @16:28:01.98 Reached 26.6 of 25.0psi after pumping 1608uL (0.93 strokes) @16:28:01.99 Archive.in :default @16:28:02.07 Archive.hold @16:28:02.81 Archive syringe pumping up to 6 strokes to reach 25psi @16:28:02.82 Archive stroke #1 of 6 @16:28:02.92 Archive.in :compress @16:28:02.98 Archive.seek :empty @16:28:08.02 Archive.in :default @16:28:08.07 Archive.hold @16:28:08.22 Initial 600uL lysate @17.6psi @16:28:08.23 Handoff.select :blocked @16:28:08.33 Initial air pressure @18.7psi @16:28:08.40 Lysis1.seek 825uL,nil @16:28:09.58 Archive syringe pumping up to 6 strokes to reach 17psi @16:28:09.59 Archive stroke #1 of 6 @16:28:09.69 Archive.in :compress @16:28:09.76 Archive.seek :empty @16:28:10.68 16.9psi after compress @16:28:18.09 Archive.in :default @16:28:18.14 Archive.hold @16:28:18.34 Recovered 825uL lysate @17.1psi @16:28:18.37 Air.in :halfSpeed @16:28:18.44 Air.seek :fullVent @16:28:20.65 Puck Top Vented, bottom @1.3psi @16:28:20.66 Handoff.select :waste @16:28:20.79 delay 5 seconds @16:28:25.85 Handoff open to waste, bottom @0.2psi @16:28:25.89 Air.in :default @16:28:25.97 Air.seek :evac @16:28:27.39 Lysis1.in :DAmix @16:28:27.49 Lysis2.in :DAmix @16:28:27.90 Lysis1.seek 575uL @16:28:27.98 Lysis2.seek 1480uL @16:29:03.02 delay 5 seconds @16:29:08.03 Handoff.select SPR @16:29:08.14 SPR.command "start 2 14" @16:29:08.90 SPR stabilized @16:29:09.03 delay 10 seconds @16:29:19.04 SPR.command "start 1 0" @16:29:22.26 Waiting up to 4:00 for SPR to enter state 1 @16:31:24.66 SPR.command "hold_on" @16:31:26.05 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @16:31:26.50 Lysis1.seek 275uL @16:31:26.55 Lysis2.seek 1180uL @16:32:08.58 delay 15 seconds @16:32:23.60 SPR.command "hold_off" @16:32:26.70 Waiting up to 38:00 for SPR to enter state 7 @16:53:16.56 SPRsummary:-141.799,-58.4227,-52.952,-66.2467 after 3143s @16:59:53.78 SPR analysis completed @16:59:59.49 Ended log spr22may1316h.spr.bz2 @16:59:59.50 SPR.command "stats_off" @17:00:01.07 -> Cmd.stop @17:00:01.17 Handoff.select :close @17:00:01.30 Gate.power :heat,:OFF @17:00:01.32 Gate.power :cartridge,:OFF @17:00:01.40 Carousel.seek 60 @17:00:01.59 Plunge.seek :locked @17:00:02.45 PinPort.in :unlocking @17:00:02.52 PinPort.seek :clear @17:00:03.06 Archive.in :unlocking @17:00:03.19 Archive.seek :clear @17:00:03.22 Air.in :unlocking @17:00:03.39 Air.seek :clear @17:00:03.48 Lysis1.in :unlocking @17:00:03.59 Lysis1.seek :clear @17:00:03.63 Lysis2.in :unlocking @17:00:03.74 Lysis2.seek :clear @17:00:07.34 Gate.power :motor,:OFF @17:00:07.36 Gate.power :core,:OFF @17:00:07.38 Gate.power :analytic1,:OFF @17:00:07.40 Gate.power :analytic2,:OFF @17:00:07.43 Gate.power :analytic3,:OFF @17:00:07.45 Gate.power :main,:OFF @17:00:07.47 Safely stopped and ready to power off :STOPPED