@23:05:45.58PDT13-May-22
>BREAK< ESP3 Gateway v1.12 for core bus @23:05:45.60 >BREAK< ESP3 Gateway v1.12 for cartridge bus @23:05:48.28 Configuration for honu by methane@mbari.org 3/16/22 /home/esp/esp2/type/auv/honu/configure.rb @23:05:55.19 Polling Can every 2:00 @23:05:55.66 Clients may connect on TCP port 9999 @23:05:55.67 Connecting to 10.89.5.2:9999 @23:05:55.70 Waiting for espclients... @23:05:56.06 Session Begun @23:05:58.00 -> showStatus true @23:06:00.03 -> Cmd.loadCartridge -10 @23:06:00.09 Gate.power :main,:ON,limit to 5000mA @23:06:00.11 Gate.power :analytic1,:ON,limit to 5000mA @23:06:00.13 Gate.power :analytic2,:ON,limit to 5000mA @23:06:00.16 Gate.power :analytic3,:ON,limit to 5000mA @23:06:00.30 Powering core bus @23:06:02.57 SPR Module 1, FW Rev. B, FPGA Rev. C @23:06:03.04 Archive.configure :default @23:06:03.17 Air.configure :default @23:06:03.28 Lysis1.configure :default @23:06:03.40 Lysis2.configure :default @23:06:03.53 Plunge.configure :default @23:06:03.66 SamplePump.configure :default @23:06:03.79 Toroid.configure :default @23:06:03.92 Carousel.configure :default @23:06:04.04 Pressure.configure :default @23:06:04.17 ExtraPump.configure :default @23:06:04.28 PinPort.configure :default @23:06:04.35 All elf controllers running firmware version 4.79 @23:06:04.38 Gate.power :motor,:ON,limit to 5000mA @23:06:04.68 Archive.in :default @23:06:04.87 Archive.home.jog 0 @23:06:05.07 Toroid.in :default @23:06:05.11 Plunge.in :default @23:06:05.15 Lysis1.in :default @23:06:05.22 Air.in :default @23:06:05.27 PinPort.in :default @23:06:05.31 Lysis2.in :default @23:06:05.46 Toroid.home.jog 0 @23:06:05.54 Plunge.home.jog 0 @23:06:05.60 PinPort.home.jog 0 @23:06:05.63 Lysis1.home.jog 0 @23:06:05.66 Air.home.jog 0 @23:06:05.71 Lysis2.home.jog 0 @23:06:06.03 Archive.in :homing @23:06:06.17 Toroid.in :homing @23:06:06.29 Archive.jog 1395 @23:06:06.37 Plunge.in :homing @23:06:06.41 PinPort.in :homing @23:06:06.53 Toroid.jog 646 @23:06:06.59 PinPort.jog -105 @23:06:06.61 Plunge.jog 1561 @23:06:06.82 Lysis2.in :homing @23:06:06.84 Lysis1.in :homing @23:06:06.90 Air.in :homing @23:06:06.99 Lysis1.jog 1377 @23:06:07.05 Lysis2.jog 1397 @23:06:07.07 Air.jog 1383 @23:06:07.24 PinPort.setRawPosition! 0 @23:06:07.29 PinPort.seek :home @23:06:07.38 PinPort.in :default @23:06:07.61 Archive.setRawPosition! 50 @23:06:07.66 Archive.seek :home @23:06:07.90 Plunge.setRawPosition! 50 @23:06:07.96 Plunge.seek :home @23:06:08.04 Archive.in :default @23:06:08.27 Plunge.in :default @23:06:08.28 Lysis1.setRawPosition! 50 @23:06:08.38 Lysis1.seek :home @23:06:08.49 Lysis2.setRawPosition! 50 @23:06:08.50 Air.setRawPosition! 50 @23:06:08.60 Lysis2.seek :home @23:06:08.61 Air.seek :home @23:06:08.77 Lysis1.in :default @23:06:08.86 Toroid.setRawPosition! 0 @23:06:08.92 Toroid.seek :home @23:06:09.04 Lysis2.in :default @23:06:09.06 Air.in :default @23:06:09.10 Toroid.in :default @23:06:09.55 Toroid.seek :clear @23:06:11.16 Carousel.in :default @23:06:11.23 Carousel.home.jog 0 @23:06:11.71 Carousel.in :default @23:06:11.77 Carousel.seek 55 @23:06:11.93 Selecting Cartridge 56 @23:06:12.19 Carousel.seek 56 @23:06:12.68 Lysis1.in :fast @23:06:12.74 Lysis1.seek :locked @23:06:15.33 Plunge.seek :locked @23:06:15.47 Air.in :fast @23:06:15.53 Air.seek :locked @23:06:17.99 Powering cartridge bus @23:06:20.59 Gate.setIR 1750,5000,0,6 @23:06:20.71 Gate.setIR 0,5000,0,6 @23:06:20.79 PuckHeater.configure :default @23:06:20.85 Gate.power :heat,:ON,limit to 5000mA @23:06:21.53 Mounted lysis Cartridge board#Q05062 @23:06:21.56 Using default puck heat loss curve for HeatingAccelInCarousel 56 @23:06:40.43 -> Cmd.startFiltering 92.07@23:06:40.66 Preparing to filter cartridge 56 92.07@23:06:40.66 Preparing to filter cartridge 56 @23:06:40.94 Priming sample loop w/100ml, bypass w/10ml @23:06:40.96 SamplePump.setPosition! 0ml @23:06:41.00 Bypass.open @23:06:42.46 Intake.open @23:06:43.18 Sea 2.1m deep (17.9psia) @23:06:43.20 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @23:06:45.77 Vacuum tare is 54% of full scale @23:06:45.91 Pressure.in :default @23:06:45.97 Pressure.hold @23:06:46.15 SamplePump.in :prime @23:06:46.21 SamplePump.seek 10ml,59 seconds @23:06:54.82 Pressure.stop @23:06:54.87 SamplePump.setPosition! 0ml @23:06:54.93 Exhaust.open @23:06:56.37 Bypass.close @23:06:57.88 Pressure.in :default @23:06:57.94 Pressure.hold @23:06:58.05 Sea 2.1m deep (17.8psia) @23:06:58.19 SamplePump.in :prime @23:06:58.26 SamplePump.seek 100ml,5:16 @23:08:26.60 Pressure.stop @23:08:26.66 Bypass.open @23:08:27.36 Intake.close @23:08:28.11 Toroid.in :hold @23:08:28.61 Toroid.seek :crack @23:08:30.12 Toroid.seek :cartridge @23:08:31.21 Toroid.in :default @23:08:31.27 Toroid.seek :clear @23:08:33.24 Pressurizing cartridge to 26psi above 17.8psia @23:08:33.26 SamplePump.setPosition! 0ml @23:08:33.31 Intake.open @23:08:33.96 Bypass.close @23:08:34.68 Exhaust.close @23:08:35.48 Pressure.in :default @23:08:35.55 Pressure.hold @23:08:35.73 SamplePump.in :charge @23:08:35.80 SamplePump.seek 2.3ml,42 seconds @23:08:49.58 Pressure.stop @23:08:49.64 Intake.close @23:08:50.34 Pressurized cartridge with 0.916ml to 26.4psi @23:08:50.35 Isolated @36.4psia (18.5psi) @23:08:50.37 Verifying cartridge holds at least 0.65..0.85 of 36.4psia @23:08:50.40 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @23:08:54.98 Cartridge pressure fell 4% to 34.8psia @23:08:55.06 Sampling 1000.0ml @23:08:55.08 SamplePump.setPosition! 0ml @23:08:55.14 Exhaust.open @23:08:55.88 Intake.open @23:08:56.68 Pressure.in :default @23:08:56.75 Pressure.hold @23:08:56.87 Sea 3.0m deep (19.2psia) @23:08:57.01 SamplePump.in :sample @23:08:57.09 SamplePump.seek 1000ml,1:23:48 @23:08:59.72 Cartridge 56 filtering 1/1000ml at 0.45ml/s with 26.2psi vacuum 3.9m deep (20.5psia) @23:09:59.71 Cartridge 56 filtering 29/1000ml at 0.49ml/s with 23.5psi vacuum 1.9m deep (17.4psia) @23:10:59.72 Cartridge 56 filtering 58/1000ml at 0.47ml/s with 22.9psi vacuum @23:11:59.71 Cartridge 56 filtering 86/1000ml at 0.49ml/s with 22.6psi vacuum 2.7m deep (18.6psia) @23:12:59.72 Cartridge 56 filtering 115/1000ml at 0.46ml/s with 23.1psi vacuum @23:13:59.71 Cartridge 56 filtering 143/1000ml at 0.47ml/s with 21.0psi vacuum 1.7m deep (17.2psia) @23:14:59.72 Cartridge 56 filtering 171/1000ml at 0.45ml/s with 22.9psi vacuum @23:15:59.72 Cartridge 56 filtering 199/1000ml at 0.48ml/s with 24.8psi vacuum @23:16:59.72 Cartridge 56 filtering 227/1000ml at 0.47ml/s with 26.4psi vacuum @23:17:59.72 Cartridge 56 filtering 255/1000ml at 0.47ml/s with 24.7psi vacuum 1.7m deep (17.2psia) @23:18:59.72 Cartridge 56 filtering 283/1000ml at 0.47ml/s with 20.9psi vacuum @23:19:59.73 Cartridge 56 filtering 310/1000ml at 0.47ml/s with 25.8psi vacuum @23:20:59.72 Cartridge 56 filtering 338/1000ml at 0.45ml/s with 25.3psi vacuum @23:21:59.71 Cartridge 56 filtering 366/1000ml at 0.44ml/s with 23.6psi vacuum @23:22:59.72 Cartridge 56 filtering 393/1000ml at 0.44ml/s with 22.6psi vacuum @23:23:59.71 Cartridge 56 filtering 421/1000ml at 0.45ml/s with 25.4psi vacuum @23:24:59.72 Cartridge 56 filtering 448/1000ml at 0.46ml/s with 25.2psi vacuum @23:25:59.72 Cartridge 56 filtering 476/1000ml at 0.46ml/s with 21.7psi vacuum @23:26:59.72 Cartridge 56 filtering 503/1000ml at 0.45ml/s with 27.7psi vacuum @23:27:59.71 Cartridge 56 filtering 531/1000ml at 0.47ml/s with 22.2psi vacuum @23:28:59.72 Cartridge 56 filtering 558/1000ml at 0.46ml/s with 25.9psi vacuum @23:29:59.72 Cartridge 56 filtering 585/1000ml at 0.44ml/s with 24.5psi vacuum @23:30:59.72 Cartridge 56 filtering 613/1000ml at 0.44ml/s with 23.6psi vacuum @23:31:59.72 Cartridge 56 filtering 640/1000ml at 0.45ml/s with 20.6psi vacuum @23:32:59.72 Cartridge 56 filtering 667/1000ml at 0.45ml/s with 20.5psi vacuum @23:33:59.72 Cartridge 56 filtering 694/1000ml at 0.45ml/s with 21.0psi vacuum @23:34:59.72 Cartridge 56 filtering 721/1000ml at 0.44ml/s with 22.4psi vacuum @23:35:59.72 Cartridge 56 filtering 749/1000ml at 0.46ml/s with 25.6psi vacuum @23:36:59.72 Cartridge 56 filtering 776/1000ml at 0.44ml/s with 23.9psi vacuum @23:37:59.72 Cartridge 56 filtering 803/1000ml at 0.47ml/s with 23.1psi vacuum 1.9m deep (17.5psia) @23:38:59.72 Cartridge 56 filtering 830/1000ml at 0.44ml/s with 23.1psi vacuum @23:39:59.72 Cartridge 56 filtering 857/1000ml at 0.46ml/s with 26.1psi vacuum @23:40:59.72 Cartridge 56 filtering 884/1000ml at 0.44ml/s with 23.0psi vacuum @23:41:59.72 Cartridge 56 filtering 911/1000ml at 0.47ml/s with 24.2psi vacuum @23:42:59.72 Cartridge 56 filtering 938/1000ml at 0.46ml/s with 25.2psi vacuum 1.6m deep (17.1psia) @23:43:59.71 Cartridge 56 filtering 965/1000ml at 0.44ml/s with 24.3psi vacuum @23:44:59.72 Cartridge 56 filtering 992/1000ml at 0.46ml/s with 26.2psi vacuum @23:45:17.06 Pressure.stop @23:45:17.18 Intake.close @23:45:17.22 Exhaust.close @23:45:17.40 Toroid.in :hold @23:45:17.47 Toroid.seek :bypass @23:45:19.08 Toroid.in :default @23:45:19.15 Toroid.seek :clear @23:45:20.76 Sampled 1000.0ml @23:45:22.32 -> Cmd.startProcessing 120.0 @23:45:23.75 SPR.command "read_sequences" @23:45:24.17 SPR.command "stats_on" @23:45:24.27 SPR.command "start 0 1" @23:45:25.01 SPR.start /var/log/esp/spr22may1323h.spr.bz2 @23:45:27.02 Handoff.select :waste @23:45:33.69 Syringe tare is 18% of full scale @23:45:33.72 PinPort.in :locking @23:45:33.79 PinPort.seek :locked @23:45:34.29 Archive.in :fast @23:45:34.33 Lysis2.in :fast @23:45:34.39 Lysis1.seek 1350uL @23:45:34.51 Archive.seek :full @23:45:34.59 Lysis2.seek :full @23:45:36.54 Plunge.seek :reagent @23:45:37.42 Evacuating puck with filter bubble point of 50psi @23:45:37.44 PinPort.seek :locked @23:45:37.53 Handoff.select :waste @23:45:44.11 Air syringe pumping up to 1 stroke to reach 8psi @23:45:44.12 Air stroke #1 of 1 @23:45:44.21 Air.in :pressurize @23:45:44.27 Air.seek :pressurized @23:45:55.85 2.2psi after pressurize @23:45:55.89 Air.in :compress @23:45:55.98 Air.seek :evac @23:46:00.07 0.4psi after compress @23:46:12.64 -0.1psi after compress (stable) @23:46:12.66 Air.in :default @23:46:12.72 Air.hold @23:46:12.92 at -0.1psi Opened to waste @23:46:12.95 Handoff.select :blocked @23:46:13.09 Archive syringe pumping up to 8 strokes to reach 50psi @23:46:13.10 Archive stroke #1 of 8 @23:46:13.18 Archive.in :pressurize @23:46:13.25 Archive.seek :pressurized @23:46:26.10 3.4psi after pressurize @23:46:26.12 Archive.in :compress @23:46:26.21 Archive.seek :empty @23:46:40.89 38.1psi after compress @23:46:53.46 38.9psi after compress (stable) @23:46:53.48 Archive.in :gulp @23:46:53.56 Archive.seek :topVent @23:47:00.73 38.9psi after gulp @23:47:13.31 38.6psi after gulp (stable) @23:47:13.32 Archive stroke #2 of 8 @23:47:13.43 Archive.in :pressurize @23:47:13.52 Archive.seek :pressurized @23:47:26.38 38.3psi after pressurize @23:47:26.40 Archive.in :compress @23:47:26.48 Archive.seek :empty @23:47:37.06 Reached 50.2 of 50.0psi after pumping 3372uL (1.95 strokes) @23:47:37.09 Archive.in :default @23:47:37.18 Archive.hold @23:47:37.24 Handoff.select :waste @23:47:44.43 delay 1:00 @23:48:44.45 Handoff.select :blocked @23:49:20.63 Handoff.select :waste @23:49:20.75 6.3psi at evacEnd @23:49:20.77 Handoff.select :blocked @23:49:20.90 Archive.in :fast @23:49:20.96 Archive.seek :topVent @23:49:45.83 Stable @4.6psi after Handoff blocked @23:49:45.85 Archive syringe pumping up to 8 strokes to reach 35psi @23:49:45.87 Archive stroke #1 of 8 @23:49:45.97 Archive.in :pressurize @23:49:46.03 Archive.seek :pressurized @23:49:58.88 5.8psi after pressurize @23:49:58.91 Archive.in :compress @23:49:59.00 Archive.seek :empty @23:50:13.67 9.6psi after compress @23:50:51.74 20.9psi after compress (stable) @23:50:51.76 Archive.in :gulp @23:50:51.83 Archive.seek :topVent @23:50:58.97 21.8psi after gulp @23:51:11.55 22.5psi after gulp (stable) @23:51:11.56 Archive stroke #2 of 8 @23:51:11.66 Archive.in :pressurize @23:51:11.72 Archive.seek :pressurized @23:51:24.58 23.1psi after pressurize @23:51:24.60 Archive.in :compress @23:51:24.67 Archive.seek :empty @23:51:39.35 26.1psi after compress @23:52:04.23 Reached 34.6 of 34.6psi after pumping 3460uL (2.00 strokes) @23:52:04.25 Archive.in :default @23:52:04.31 Archive.hold @23:52:04.39 Handoff.select :waste @23:52:11.56 delay 1:00 @23:53:11.57 Handoff.select :blocked @23:53:34.25 Handoff.select :waste @23:53:34.37 2.5psi at evacEnd @23:53:34.38 Air.seek :fullVent @23:53:35.81 Archive.in :fast @23:53:35.87 Archive.seek :topVent @23:53:42.97 Seawater evacuated, Presure above/below filter released. Lysis begins @23:53:43.05 Lysis1.in :slow @23:53:43.13 Lysis1.seek 1050uL @23:54:13.16 delay 5 seconds @23:54:18.17 Handoff.select :blocked @23:54:18.34 Lysis1.seek 450uL @23:55:18.28 delay 3 seconds @23:55:21.35 Air.in mix with 1.40048 parts Lysis1 @23:55:21.80 Air.seek :evac @23:55:21.84 Lysis1.seek 300uL @23:55:36.95 Lysis1.seek :empty @23:56:07.03 Lysis buffer delivered @20.0psi @23:56:07.05 Polling Can every 2:00 @23:56:07.60 PuckHeater.configure :default #ambient 21.9C @23:56:07.65 PuckHeater.seek 79.7C @23:57:00.21 PuckHeater.hold 2:00,79.7C @23:59:00.22 Polling Can every 2:00 @23:59:00.75 PuckHeater.configure :default #ambient 21.9C @23:59:00.80 PuckHeater.hold 30 seconds,78.7C @23:59:30.98 Polling Can every 2:00 @23:59:31.52 PuckHeater.configure :default #ambient 21.9C @23:59:31.57 PuckHeater.hold 30 seconds,78.2C @00:00:01.75PDT14-May-22 Polling Can every 2:00 @00:00:02.29 PuckHeater.hold 10:00,77.7C @00:10:01.65 Lysis complete. Syringe pressure @23.7psi @00:10:01.66 Handoff.select :blocked @00:10:01.71 Archive syringe pumping up to 5 strokes to reach 40psi @00:10:01.72 Archive stroke #1 of 5 @00:10:01.82 Archive.in :pressurize @00:10:01.88 Archive.seek :pressurized @00:10:14.73 23.4psi after pressurize @00:10:14.74 Archive.in :compress @00:10:14.82 Archive.seek :empty @00:10:21.73 Reached 40.6 of 40.0psi after pumping 1563uL (0.90 strokes) @00:10:21.75 Archive.in :default @00:10:21.82 Archive.hold @00:10:21.88 delay 2 seconds @00:10:23.92 Archive.in :fast @00:10:23.98 Archive.seek :full @00:10:30.57 Lysis1.seek 138uL @00:10:44.43 delay 1 second @00:10:45.46 Archive.seek :full @00:10:45.51 Lysis1.seek :empty @00:10:59.53 Initial air pressure @44.0psi @00:10:59.60 Lysis1.in :extract @00:10:59.68 Lysis1.seek 600uL,nil @00:11:01.94 Archive syringe pumping up to 6 strokes to reach 25psi @00:11:01.95 Archive stroke #1 of 6 @00:11:02.04 Archive.in :pressurize @00:11:02.11 Archive.seek :pressurized @00:11:14.99 15.8psi after pressurize @00:11:15.00 Archive.in :compress @00:11:15.08 Archive.seek :empty @00:11:21.40 Syringe::LysatePlungerStuck in PROCESSING -- at < 266uL during Lysis Syringe #1 (LyS1) move @00:11:21.43 Lysis1.in :default @00:11:21.52 Lysis1.seek 231uL @00:11:22.11 Reached 27.8 of 25.0psi after pumping 1565uL (0.90 strokes) @00:11:22.13 Archive.in :default @00:11:22.20 Archive.hold @00:11:23.36 Lysis1.in :extract @00:11:23.48 Lysis1.seek 600uL,nil @00:11:27.04 Archive syringe pumping up to 6 strokes to reach 25psi @00:11:27.05 Archive stroke #1 of 6 @00:11:27.14 Archive.in :compress @00:11:27.25 Archive.seek :empty @00:11:31.02 Archive.in :default @00:11:31.55 Archive.hold @00:11:31.71 Initial 600uL lysate @22.1psi @00:11:31.72 Handoff.select :blocked @00:11:31.82 Initial air pressure @22.5psi @00:11:31.89 Lysis1.seek 825uL,nil @00:11:33.70 Archive syringe pumping up to 6 strokes to reach 17psi @00:11:33.71 Archive stroke #1 of 6 @00:11:33.89 Archive.in :compress @00:11:33.96 Archive.seek :empty @00:11:37.37 Archive.in :default @00:11:37.42 Archive.hold @00:11:37.66 Recovered 825uL lysate @17.3psi @00:11:37.68 Air.in :halfSpeed @00:11:37.75 Air.seek :fullVent @00:11:39.96 Puck Top Vented, bottom @21.4psi @00:11:39.97 Handoff.select :waste @00:11:40.10 delay 5 seconds @00:11:45.19 Handoff open to waste, bottom @0.1psi @00:11:45.21 Air.in :default @00:11:45.29 Air.seek :evac @00:11:46.70 Lysis1.in :DAmix @00:11:46.81 Lysis2.in :DAmix @00:11:47.26 Lysis1.seek 575uL @00:11:47.31 Lysis2.seek 1480uL @00:12:22.39 delay 5 seconds @00:12:27.40 Handoff.select SPR @00:12:27.53 SPR.command "start 2 14" @00:12:28.17 SPR stabilized @00:12:28.31 delay 10 seconds @00:12:38.33 SPR.command "start 1 0" @00:12:41.54 Waiting up to 4:00 for SPR to enter state 1 @00:14:43.94 SPR.command "hold_on" @00:14:45.32 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @00:14:45.80 Lysis1.seek 275uL @00:14:45.84 Lysis2.seek 1180uL @00:15:27.87 delay 15 seconds @00:15:42.89 SPR.command "hold_off" @00:15:45.97 Waiting up to 38:00 for SPR to enter state 7 @00:36:35.84 SPRsummary:-20.828,24.6773,29.3467,33.1507 after 3219s @00:43:13.06 SPR analysis completed @00:43:19.00 Ended log spr22may1323h.spr.bz2 @00:43:19.02 SPR.command "stats_off" @00:43:20.53 -> Cmd.stop @00:43:20.63 Handoff.select :close @00:43:20.76 Gate.power :heat,:OFF @00:43:20.78 Gate.power :cartridge,:OFF @00:43:20.86 Carousel.seek 56 @00:43:21.05 Plunge.seek :locked @00:43:21.90 PinPort.in :unlocking @00:43:21.97 PinPort.seek :clear @00:43:22.52 Archive.in :unlocking @00:43:22.65 Archive.seek :clear @00:43:22.70 Air.in :unlocking @00:43:22.85 Air.seek :clear @00:43:22.89 Lysis1.in :unlocking @00:43:23.02 Lysis1.seek :clear @00:43:23.15 Lysis2.in :unlocking @00:43:23.22 Lysis2.seek :clear @00:43:26.77 Gate.power :motor,:OFF @00:43:26.79 Gate.power :core,:OFF @00:43:26.81 Gate.power :analytic1,:OFF @00:43:26.84 Gate.power :analytic2,:OFF @00:43:26.86 Gate.power :analytic3,:OFF @00:43:26.88 Gate.power :main,:OFF @00:43:26.91 Safely stopped and ready to power off :STOPPED