@17:35:23.28PDT27-May-22
>BREAK< ESP3 Gateway v1.12 for core bus @17:35:23.30 >BREAK< ESP3 Gateway v1.12 for cartridge bus @17:35:26.08 Configuration for honu by methane@mbari.org 3/16/22 /home/esp/esp2/type/auv/honu/configure.rb @17:35:37.93 Polling Can every 2:00 @17:35:38.40 Clients may connect on TCP port 9999 @17:35:38.42 Connecting to 10.89.5.2:9999 @17:35:38.46 Waiting for espclients... @17:35:38.62 Session Begun @17:35:40.56 -> showStatus true @17:35:42.58 -> Cmd.loadCartridge -10 @17:35:42.65 Gate.power :main,:ON,limit to 5000mA @17:35:42.67 Gate.power :analytic1,:ON,limit to 5000mA @17:35:42.69 Gate.power :analytic2,:ON,limit to 5000mA @17:35:42.72 Gate.power :analytic3,:ON,limit to 5000mA @17:35:42.87 Powering core bus @17:35:45.12 SPR Module 1, FW Rev. B, FPGA Rev. C @17:35:45.59 Archive.configure :default @17:35:45.82 Air.configure :default @17:35:46.04 Lysis1.configure :default @17:35:46.27 Lysis2.configure :default @17:35:46.56 Plunge.configure :default @17:35:46.85 SamplePump.configure :default @17:35:47.15 Toroid.configure :default @17:35:47.33 Carousel.configure :default @17:35:47.46 Pressure.configure :default @17:35:47.59 ExtraPump.configure :default @17:35:47.69 PinPort.configure :default @17:35:47.75 All elf controllers running firmware version 4.79 @17:35:47.77 Gate.power :motor,:ON,limit to 5000mA @17:35:47.90 Archive.in :default @17:35:48.25 Archive.home.jog 0 @17:35:48.45 Toroid.in :default @17:35:48.49 Plunge.in :default @17:35:48.53 Lysis1.in :default @17:35:48.59 Air.in :default @17:35:48.64 PinPort.in :default @17:35:48.69 Lysis2.in :default @17:35:48.90 Toroid.home.jog 0 @17:35:48.93 Plunge.home.jog 0 @17:35:48.96 PinPort.home.jog 0 @17:35:48.98 Air.home.jog 0 @17:35:49.01 Lysis1.home.jog 0 @17:35:49.04 Lysis2.home.jog 0 @17:35:49.57 Archive.in :homing @17:35:49.79 Toroid.in :homing @17:35:49.83 Plunge.in :homing @17:35:49.86 Archive.jog 1389 @17:35:49.90 Lysis1.in :homing @17:35:49.93 Air.in :homing @17:35:49.97 PinPort.in :homing @17:35:50.00 Lysis2.in :homing @17:35:50.24 Toroid.jog 640 @17:35:50.28 Plunge.jog 1554 @17:35:50.32 Lysis1.jog 1382 @17:35:50.34 PinPort.jog -107 @17:35:50.38 Air.jog 1382 @17:35:50.41 Lysis2.jog 1395 @17:35:50.73 PinPort.setRawPosition! 0 @17:35:50.78 PinPort.seek :home @17:35:50.87 PinPort.in :default @17:35:51.21 Archive.setRawPosition! 50 @17:35:51.26 Archive.seek :home @17:35:51.53 Archive.in :default @17:35:51.64 Lysis1.setRawPosition! 50 @17:35:51.66 Plunge.setRawPosition! 50 @17:35:51.77 Lysis1.seek :home @17:35:51.79 Plunge.seek :home @17:35:51.94 Lysis2.setRawPosition! 50 @17:35:51.95 Air.setRawPosition! 50 @17:35:52.04 Lysis2.seek :home @17:35:52.07 Air.seek :home @17:35:52.23 Plunge.in :default @17:35:52.25 Lysis1.in :default @17:35:52.41 Lysis2.in :default @17:35:52.49 Air.in :default @17:35:52.62 Toroid.setRawPosition! 0 @17:35:52.68 Toroid.seek :home @17:35:52.77 Toroid.in :default @17:35:52.92 Toroid.seek :clear @17:35:54.53 Carousel.in :default @17:35:54.60 Carousel.home.jog 0 @17:35:55.09 Carousel.in :default @17:35:55.16 Carousel.seek 42 @17:35:55.33 Selecting Cartridge 41 @17:35:55.59 Carousel.seek 41 @17:35:56.07 Lysis1.in :fast @17:35:56.13 Lysis1.seek :locked @17:35:58.73 Plunge.seek :locked @17:35:58.86 Air.in :fast @17:35:58.92 Air.seek :locked @17:36:01.38 Powering cartridge bus @17:36:03.98 Gate.setIR 1750,5000,0,6 @17:36:04.10 Gate.setIR 0,5000,0,6 @17:36:04.17 PuckHeater.configure :default @17:36:04.23 Gate.power :heat,:ON,limit to 5000mA @17:36:04.96 Mounted lysis Cartridge board#Q05078 @17:36:05.00 Ignoring this cartridge's HeatingAccelInCarousel heat loss curve 41 @17:36:22.98 -> Cmd.startFiltering @17:36:23.22 Preparing to filter cartridge 41 92.07 @17:36:23.89 Priming sample loop w/100ml, bypass w/10ml @17:36:23.90 SamplePump.setPosition! 0ml @17:36:23.95 Bypass.open @17:36:25.40 Intake.open @17:36:26.17 Sea 16.7m deep (39.4psia) @17:36:26.19 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:36:28.76 Vacuum tare is 54% of full scale @17:36:28.90 Intake.close @17:36:28.96 Bypass.close @17:36:29.70 Verifying toroid holds at least 0.6..0.85 of 39.3psia @17:36:29.72 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @17:36:34.29 Bypass.open @17:36:34.97 Intake.open @17:36:36.40 Pressure.in :default @17:36:36.46 Pressure.hold @17:36:36.64 SamplePump.in :prime @17:36:36.71 SamplePump.seek 10ml,59 seconds @17:36:45.33 Pressure.stop @17:36:45.38 SamplePump.setPosition! 0ml @17:36:45.43 Exhaust.open @17:36:46.89 Bypass.close @17:36:48.36 Pressure.in :default @17:36:48.42 Pressure.hold @17:36:48.55 Sea 16.2m deep (38.6psia) @17:36:48.68 SamplePump.in :prime @17:36:48.75 SamplePump.seek 100ml,5:16 @17:38:23.03 Pressure.stop @17:38:23.09 Bypass.open @17:38:23.77 Intake.close @17:38:24.53 Toroid.in :hold @17:38:24.59 Toroid.seek :crack @17:38:26.15 Toroid.seek :cartridge @17:38:27.24 Toroid.in :default @17:38:27.30 Toroid.seek :clear @17:38:29.27 Exhaust.close @17:38:29.34 Bypass.close @17:38:30.06 Verifying cartridge holds at least 0.65..0.85 of 37.5psia @17:38:30.08 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @17:38:34.70 Sampling 1000.0ml @17:38:34.71 SamplePump.setPosition! 0ml @17:38:34.77 Exhaust.open @17:38:35.52 Intake.open @17:38:36.32 Pressure.in :default @17:38:36.43 Pressure.hold @17:38:36.54 Sea 15.7m deep (37.8psia) @17:38:36.68 SamplePump.in :sample @17:38:36.75 SamplePump.seek 1000ml,1:23:48 @17:38:39.36 Cartridge 41 filtering 1/1000ml at 0.40ml/s with 26.1psi vacuum 13.5m deep (34.7psia) @17:39:39.35 Cartridge 41 filtering 26/1000ml at 0.44ml/s with 26.5psi vacuum 17.6m deep (40.7psia) @17:40:39.37 Cartridge 41 filtering 51/1000ml at 0.41ml/s with 25.8psi vacuum 16.7m deep (39.4psia) @17:41:39.36 Cartridge 41 filtering 74/1000ml at 0.39ml/s with 24.4psi vacuum 15.1m deep (37.0psia) @17:42:39.37 Cartridge 41 filtering 95/1000ml at 0.33ml/s with 24.5psi vacuum 16.4m deep (38.9psia) @17:43:39.36 Cartridge 41 filtering 117/1000ml at 0.32ml/s with 23.1psi vacuum 14.6m deep (36.3psia) @17:44:39.37 Cartridge 41 filtering 137/1000ml at 0.32ml/s with 21.9psi vacuum 15.2m deep (37.1psia) @17:45:39.36 Cartridge 41 filtering 157/1000ml at 0.35ml/s with 23.1psi vacuum 17.8m deep (41.0psia) @17:46:39.37 Cartridge 41 filtering 176/1000ml at 0.33ml/s with 21.2psi vacuum 15.9m deep (38.2psia) @17:47:39.36 Cartridge 41 filtering 195/1000ml at 0.30ml/s with 24.5psi vacuum 18.1m deep (41.5psia) @17:48:39.37 Cartridge 41 filtering 213/1000ml at 0.29ml/s with 23.1psi vacuum 14.6m deep (36.3psia) @17:49:39.36 Cartridge 41 filtering 231/1000ml at 0.32ml/s with 22.8psi vacuum 15.6m deep (37.8psia) @17:50:39.37 Cartridge 41 filtering 249/1000ml at 0.32ml/s with 24.6psi vacuum 17.4m deep (40.4psia) @17:51:39.35 Cartridge 41 filtering 267/1000ml at 0.31ml/s with 23.1psi vacuum 17.5m deep (40.6psia) @17:52:39.37 Cartridge 41 filtering 284/1000ml at 0.28ml/s with 22.1psi vacuum 15.5m deep (37.6psia) @17:53:39.36 Cartridge 41 filtering 300/1000ml at 0.27ml/s with 23.0psi vacuum 14.6m deep (36.3psia) @17:54:39.36 Cartridge 41 filtering 317/1000ml at 0.25ml/s with 23.6psi vacuum 15.1m deep (37.0psia) @17:55:39.36 Cartridge 41 filtering 333/1000ml at 0.26ml/s with 22.7psi vacuum 16.3m deep (38.8psia) @17:56:39.36 Cartridge 41 filtering 349/1000ml at 0.26ml/s with 20.6psi vacuum 15.8m deep (38.1psia) @17:57:39.36 Cartridge 41 filtering 364/1000ml at 0.25ml/s with 26.0psi vacuum 15.3m deep (37.3psia) @17:58:39.36 Cartridge 41 filtering 379/1000ml at 0.27ml/s with 21.1psi vacuum 16.4m deep (39.0psia) @17:59:39.40 Cartridge 41 filtering 395/1000ml at 0.24ml/s with 21.8psi vacuum 17.6m deep (40.7psia) @18:00:39.36 Cartridge 41 filtering 409/1000ml at 0.24ml/s with 21.8psi vacuum 16.4m deep (38.9psia) @18:01:39.36 Cartridge 41 filtering 424/1000ml at 0.26ml/s with 23.0psi vacuum 15.5m deep (37.7psia) @18:02:39.36 Cartridge 41 filtering 438/1000ml at 0.22ml/s with 23.4psi vacuum 15.9m deep (38.2psia) @18:03:39.37 Cartridge 41 filtering 452/1000ml at 0.22ml/s with 24.2psi vacuum 14.8m deep (36.5psia) @18:04:39.36 Cartridge 41 filtering 466/1000ml at 0.22ml/s with 22.2psi vacuum 16.0m deep (38.3psia) @18:05:39.36 Cartridge 41 filtering 480/1000ml at 0.21ml/s with 22.8psi vacuum 16.0m deep (38.4psia) @18:06:39.36 Cartridge 41 filtering 493/1000ml at 0.22ml/s with 24.0psi vacuum 15.8m deep (38.0psia) @18:07:39.36 Cartridge 41 filtering 506/1000ml at 0.21ml/s with 25.5psi vacuum 15.4m deep (37.4psia) @18:08:39.37 Cartridge 41 filtering 519/1000ml at 0.23ml/s with 26.7psi vacuum 15.2m deep (37.1psia) @18:09:39.36 Cartridge 41 filtering 532/1000ml at 0.21ml/s with 25.5psi vacuum 15.9m deep (38.2psia) @18:10:39.37 Cartridge 41 filtering 545/1000ml at 0.23ml/s with 24.7psi vacuum 16.4m deep (39.0psia) @18:11:39.36 Cartridge 41 filtering 558/1000ml at 0.20ml/s with 27.3psi vacuum 15.4m deep (37.4psia) @18:12:39.37 Cartridge 41 filtering 570/1000ml at 0.20ml/s with 26.3psi vacuum 14.9m deep (36.8psia) @18:13:39.36 Cartridge 41 filtering 582/1000ml at 0.21ml/s with 26.1psi vacuum 16.1m deep (38.5psia) @18:14:39.36 Cartridge 41 filtering 595/1000ml at 0.20ml/s with 27.0psi vacuum 14.7m deep (36.4psia) @18:15:39.36 Cartridge 41 filtering 607/1000ml at 0.21ml/s with 23.1psi vacuum 15.6m deep (37.7psia) @18:16:39.37 Cartridge 41 filtering 619/1000ml at 0.20ml/s with 26.7psi vacuum 15.4m deep (37.4psia) @18:17:39.36 Cartridge 41 filtering 631/1000ml at 0.20ml/s with 23.8psi vacuum 16.4m deep (39.0psia) @18:17:54.82 Flow averaged 0.27ml/s over 39:19 @18:17:54.86 Waiting up to 8 seconds for vacuum to reduce from 26.1 to 18.0psi @18:17:55.44 Relieved excess vacuum in .6 seconds @18:17:55.46 SamplePump.seek 1000ml,30:57 @18:18:39.35 Cartridge 41 filtering 643/1000ml at 0.20ml/s with 25.7psi vacuum 15.9m deep (38.2psia) @18:19:35.92 Flow averaged 0.20ml/s over 1:41 @18:19:35.96 Waiting up to 9 seconds for vacuum to reduce from 26.3 to 18.0psi @18:19:36.53 Relieved excess vacuum in .6 seconds @18:19:36.56 SamplePump.seek 1000ml,29:16 @18:19:39.35 Cartridge 41 filtering 655/1000ml at 0.20ml/s with 26.9psi vacuum 14.3m deep (35.9psia) @18:20:39.37 Cartridge 41 filtering 667/1000ml at 0.20ml/s with 27.1psi vacuum 15.3m deep (37.4psia) @18:21:09.09 Flow averaged 0.20ml/s over 1:33 @18:21:09.12 Waiting up to 11 seconds for vacuum to reduce from 27.2 to 18.0psi @18:21:09.70 Relieved excess vacuum in .6 seconds @18:21:10.21 SamplePump.seek 1000ml,27:42 @18:21:39.37 Cartridge 41 filtering 679/1000ml at 0.20ml/s with 27.6psi vacuum 15.4m deep (37.4psia) @18:21:57.56 Flow averaged 0.20ml/s over 48 seconds @18:21:57.59 Intake.close @18:21:57.63 Exhaust.close @18:21:57.84 Toroid.in :hold @18:21:57.90 Toroid.seek :bypass @18:21:59.52 Toroid.in :default @18:21:59.58 Toroid.seek :clear @18:22:01.20 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @18:22:01.21 Pressure.stop @18:22:01.45 Sampled 683.0ml @18:22:03.40 -> Cmd.startProcessing 120.0 @18:22:04.84 SPR.command "read_sequences" @18:22:04.88 SPR.command "stats_on" @18:22:04.99 SPR.command "start 0 1" @18:22:05.73 SPR.start /var/log/esp/spr22may2718h.spr.bz2 @18:22:07.64 Handoff.select :waste @18:22:14.33 Syringe tare is 18% of full scale @18:22:14.35 PinPort.in :locking @18:22:14.42 PinPort.seek :locked @18:22:14.92 Archive.in :fast @18:22:14.97 Lysis2.in :fast @18:22:15.01 Lysis1.seek 1350uL @18:22:15.14 Archive.seek :full @18:22:15.23 Lysis2.seek :full @18:22:17.18 Plunge.seek :reagent @18:22:18.05 Evacuating puck with filter bubble point of 50psi @18:22:18.07 PinPort.seek :locked @18:22:18.17 Handoff.select :waste @18:22:24.75 Air syringe pumping up to 1 stroke to reach 8psi @18:22:24.76 Air stroke #1 of 1 @18:22:24.85 Air.in :pressurize @18:22:24.91 Air.seek :pressurized @18:22:36.51 0.4psi after pressurize @18:22:36.53 Air.in :compress @18:22:36.61 Air.seek :evac @18:22:40.68 1.2psi after compress @18:22:53.25 0.1psi after compress (stable) @18:22:53.27 Air.in :default @18:22:53.34 Air.hold @18:22:53.59 at 0.0psi Opened to waste @18:22:53.60 Handoff.select :blocked @18:22:53.74 Archive syringe pumping up to 8 strokes to reach 50psi @18:22:53.75 Archive stroke #1 of 8 @18:22:53.84 Archive.in :pressurize @18:22:53.90 Archive.seek :pressurized @18:23:06.80 6.6psi after pressurize @18:23:06.82 Archive.in :compress @18:23:06.91 Archive.seek :empty @18:23:21.60 26.2psi after compress @18:23:34.19 28.3psi after compress (stable) @18:23:34.22 Archive.in :gulp @18:23:34.31 Archive.seek :topVent @18:23:41.47 28.4psi after gulp @18:23:54.06 28.4psi after gulp (stable) @18:23:54.08 Archive stroke #2 of 8 @18:23:54.18 Archive.in :pressurize @18:23:54.25 Archive.seek :pressurized @18:24:07.10 28.3psi after pressurize @18:24:07.12 Archive.in :compress @18:24:07.20 Archive.seek :empty @18:24:21.89 47.0psi after compress @18:24:34.46 48.5psi after compress (stable) @18:24:34.48 Archive.in :gulp @18:24:34.55 Archive.seek :topVent @18:24:41.72 48.4psi after gulp @18:24:54.29 48.4psi after gulp (stable) @18:24:54.30 Archive stroke #3 of 8 @18:24:54.39 Archive.in :pressurize @18:24:54.46 Archive.seek :pressurized @18:25:07.31 48.4psi after pressurize @18:25:07.33 Archive.in :compress @18:25:07.41 Archive.seek :empty @18:25:14.33 Reached 50.2 of 50.0psi after pumping 5023uL (2.90 strokes) @18:25:14.34 Archive.in :default @18:25:14.43 Archive.hold @18:25:14.51 Handoff.select :waste @18:25:25.19 delay 1:00 @18:26:25.21 Handoff.select :blocked @18:26:43.94 Pressure too High, open to waste again (8.1psi) @18:26:43.95 Handoff.select :waste @18:26:44.09 delay 1:00 @18:27:44.10 Handoff.select :blocked @18:28:16.79 Handoff.select :waste @18:28:16.91 5.2psi at evacEnd @18:28:16.92 Handoff.select :blocked @18:28:17.08 Archive.in :fast @18:28:17.14 Archive.seek :topVent @18:28:37.23 Stable @3.1psi after Handoff blocked @18:28:37.25 Archive syringe pumping up to 8 strokes to reach 33psi @18:28:37.26 Archive stroke #1 of 8 @18:28:37.36 Archive.in :pressurize @18:28:37.43 Archive.seek :pressurized @18:28:50.28 4.6psi after pressurize @18:28:50.30 Archive.in :compress @18:28:50.37 Archive.seek :empty @18:29:05.06 8.7psi after compress @18:29:45.14 18.9psi after compress (stable) @18:29:45.16 Archive.in :gulp @18:29:45.24 Archive.seek :topVent @18:29:52.39 19.8psi after gulp @18:30:04.97 20.3psi after gulp (stable) @18:30:04.98 Archive stroke #2 of 8 @18:30:05.07 Archive.in :pressurize @18:30:05.14 Archive.seek :pressurized @18:30:17.99 20.7psi after pressurize @18:30:18.02 Archive.in :compress @18:30:18.17 Archive.seek :empty @18:30:32.86 24.3psi after compress @18:31:00.25 Reached 33.1 of 33.1psi after pumping 3460uL (2.00 strokes) @18:31:00.26 Archive.in :default @18:31:00.33 Archive.hold @18:31:00.40 Handoff.select :waste @18:31:07.58 delay 1:00 @18:32:07.60 Handoff.select :blocked @18:32:42.32 Pressure too High, open to waste again (8.1psi) @18:32:42.33 Handoff.select :waste @18:32:42.47 delay 1:00 @18:33:42.48 Handoff.select :blocked @18:34:05.16 Handoff.select :waste @18:34:05.27 2.1psi at evacEnd @18:34:05.29 Air.seek :fullVent @18:34:06.72 Archive.in :fast @18:34:06.78 Archive.seek :topVent @18:34:13.89 Seawater evacuated, Presure above/below filter released. Lysis begins @18:34:14.26 Lysis1.in :slow @18:34:14.39 Lysis1.seek 1050uL @18:34:44.43 delay 5 seconds @18:34:49.45 Handoff.select :blocked @18:34:49.65 Lysis1.seek 450uL @18:35:49.62 delay 3 seconds @18:35:52.69 Air.in mix with 1.40048 parts Lysis1 @18:35:53.16 Air.seek :evac @18:35:53.24 Lysis1.seek 300uL @18:36:08.32 Lysis1.seek :empty @18:36:38.42 Lysis buffer delivered @18.1psi @18:36:38.43 Polling Can every 2:00 @18:36:38.98 PuckHeater.configure :default #ambient 13.9C @18:36:39.03 PuckHeater.seek 80.1C @18:37:36.93 PuckHeater.hold 2:00,80.1C @18:39:36.76 Polling Can every 2:00 @18:39:37.30 PuckHeater.configure :default #ambient 14.0C @18:39:37.35 PuckHeater.hold 30 seconds,79.1C @18:40:07.50 Polling Can every 2:00 @18:40:08.04 PuckHeater.configure :default #ambient 14.0C @18:40:08.09 PuckHeater.hold 30 seconds,78.6C @18:40:38.24 Polling Can every 2:00 @18:40:38.78 PuckHeater.configure :default #ambient 14.0C @18:40:38.83 PuckHeater.hold 10:00,78C @18:50:38.01 Lysis complete. Syringe pressure @17.6psi @18:50:38.02 Handoff.select :blocked @18:50:38.08 Archive syringe pumping up to 5 strokes to reach 40psi @18:50:38.09 Archive stroke #1 of 5 @18:50:38.20 Archive.in :pressurize @18:50:38.27 Archive.seek :pressurized @18:50:51.13 17.4psi after pressurize @18:50:51.14 Archive.in :compress @18:50:51.22 Archive.seek :empty @18:50:59.84 Reached 40.5 of 40.0psi after pumping 1599uL (0.92 strokes) @18:50:59.85 Archive.in :default @18:50:59.94 Archive.hold @18:51:00.02 delay 2 seconds @18:51:02.04 Archive.in :fast @18:51:02.10 Archive.seek :full @18:51:08.82 Lysis1.seek 138uL @18:51:22.70 delay 1 second @18:51:23.73 Archive.seek :full @18:51:23.76 Lysis1.seek :empty @18:51:37.79 Initial air pressure @43.1psi @18:51:37.87 Lysis1.in :extract @18:51:37.95 Lysis1.seek 600uL,nil @18:51:42.57 Archive syringe pumping up to 6 strokes to reach 25psi @18:51:42.58 Archive stroke #1 of 6 @18:51:42.69 Archive.in :pressurize @18:51:42.76 Archive.seek :pressurized @18:51:55.61 15.6psi after pressurize @18:51:55.68 Archive.in :compress @18:51:55.78 Archive.seek :empty @18:52:02.26 Syringe::LysatePlungerStuck in PROCESSING -- at < 306uL during Lysis Syringe #1 (LyS1) move @18:52:02.29 Lysis1.in :default @18:52:02.37 Lysis1.seek 271uL @18:52:03.06 Reached 27.2 of 25.0psi after pumping 1570uL (0.91 strokes) @18:52:03.07 Archive.in :default @18:52:03.14 Archive.hold @18:52:04.21 Lysis1.in :extract @18:52:04.29 Lysis1.seek 600uL,nil @18:52:10.31 Initial 600uL lysate @17.6psi @18:52:10.33 Handoff.select :blocked @18:52:10.43 Initial air pressure @18.8psi @18:52:10.50 Lysis1.seek 825uL,nil @18:52:12.24 Archive syringe pumping up to 6 strokes to reach 17psi @18:52:12.25 Archive stroke #1 of 6 @18:52:12.35 Archive.in :compress @18:52:12.41 Archive.seek :empty @18:52:17.33 Archive.in :default @18:52:17.38 Archive.hold @18:52:17.62 Recovered 825uL lysate @16.7psi @18:52:17.64 Air.in :halfSpeed @18:52:17.71 Air.seek :fullVent @18:52:19.92 Puck Top Vented, bottom @8.1psi @18:52:19.93 Handoff.select :waste @18:52:20.06 delay 5 seconds @18:52:25.12 Handoff open to waste, bottom @0.2psi @18:52:25.14 Air.in :default @18:52:25.21 Air.seek :evac @18:52:26.62 Lysis1.in :DAmix @18:52:26.74 Lysis2.in :DAmix @18:52:27.18 Lysis1.seek 575uL @18:52:27.23 Lysis2.seek 1480uL @18:53:02.31 delay 5 seconds @18:53:07.33 Handoff.select SPR @18:53:07.45 SPR.command "start 2 14" @18:53:08.58 SPR stabilized @18:53:08.71 delay 10 seconds @18:53:18.72 SPR.command "start 1 0" @18:53:21.45 Waiting up to 4:00 for SPR to enter state 1 @18:55:23.83 SPR.command "hold_on" @18:55:25.24 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:55:25.72 Lysis1.seek 275uL @18:55:25.76 Lysis2.seek 1180uL @18:56:07.78 delay 15 seconds @18:56:22.79 SPR.command "hold_off" @18:56:25.89 Waiting up to 38:00 for SPR to enter state 7 @19:17:15.72 SPRsummary:-14.12,97.4133,99.652,89.4187 after 3472s @19:23:52.95 SPR analysis completed @19:23:59.84 Ended log spr22may2718h.spr.bz2 @19:23:59.86 SPR.command "stats_off" @19:24:01.14 -> Cmd.stop @19:24:01.24 Handoff.select :close @19:24:01.37 Gate.power :heat,:OFF @19:24:01.38 Gate.power :cartridge,:OFF @19:24:01.47 Carousel.seek 41 @19:24:01.65 Plunge.seek :locked @19:24:02.51 PinPort.in :unlocking @19:24:02.57 PinPort.seek :clear @19:24:03.11 Archive.in :unlocking @19:24:03.24 Archive.seek :clear @19:24:03.27 Air.in :unlocking @19:24:03.44 Air.seek :clear @19:24:03.54 Lysis1.in :unlocking @19:24:03.67 Lysis1.seek :clear @19:24:03.70 Lysis2.in :unlocking @19:24:03.81 Lysis2.seek :clear @19:24:07.40 Gate.power :motor,:OFF @19:24:07.42 Gate.power :core,:OFF @19:24:07.44 Gate.power :analytic1,:OFF @19:24:07.46 Gate.power :analytic2,:OFF @19:24:07.48 Gate.power :analytic3,:OFF @19:24:07.51 Gate.power :main,:OFF @19:24:07.54 Safely stopped and ready to power off :STOPPED