@16:16:50.17PDT02-Jun-22
>BREAK< ESP3 Gateway v1.12 for core bus @16:16:50.19 >BREAK< ESP3 Gateway v1.12 for cartridge bus @16:16:52.87 Configuration for honu by methane@mbari.org 3/16/22 /home/esp/esp2/type/auv/honu/configure.rb @16:17:02.16 Polling Can every 2:00 @16:17:03.45 Clients may connect on TCP port 9999 @16:17:03.47 Connecting to 10.89.5.2:9999 @16:17:03.54 Waiting for espclients... @16:17:04.06 Session Begun @16:17:05.68 -> showStatus true @16:17:07.70 -> Cmd.loadCartridge -10 @16:17:07.77 Gate.power :main,:ON,limit to 5000mA @16:17:07.79 Gate.power :analytic1,:ON,limit to 5000mA @16:17:07.81 Gate.power :analytic2,:ON,limit to 5000mA @16:17:07.84 Gate.power :analytic3,:ON,limit to 5000mA @16:17:07.98 Powering core bus @16:17:10.24 SPR Module 1, FW Rev. B, FPGA Rev. C @16:17:10.72 Archive.configure :default @16:17:10.85 Air.configure :default @16:17:10.95 Lysis1.configure :default @16:17:11.05 Lysis2.configure :default @16:17:11.18 Plunge.configure :default @16:17:11.32 SamplePump.configure :default @16:17:11.44 Toroid.configure :default @16:17:11.57 Carousel.configure :default @16:17:11.69 Pressure.configure :default @16:17:11.82 ExtraPump.configure :default @16:17:11.92 PinPort.configure :default @16:17:11.98 All elf controllers running firmware version 4.79 @16:17:12.01 Gate.power :motor,:ON,limit to 5000mA @16:17:12.21 Archive.in :default @16:17:12.50 Archive.home.jog 0 @16:17:12.66 Toroid.in :default @16:17:12.74 Plunge.in :default @16:17:12.77 Lysis1.in :default @16:17:12.83 Air.in :default @16:17:12.88 PinPort.in :default @16:17:12.91 Lysis2.in :default @16:17:13.09 Toroid.home.jog 0 @16:17:13.16 PinPort.home.jog 0 @16:17:13.18 Lysis2.home.jog 0 @16:17:13.21 Lysis1.home.jog 0 @16:17:13.23 Air.home.jog 0 @16:17:13.26 Plunge.home.jog 0 @16:17:13.72 Archive.in :homing @16:17:14.01 Toroid.in :homing @16:17:14.05 Plunge.in :homing @16:17:14.08 Archive.jog 1391 @16:17:14.13 PinPort.in :homing @16:17:14.16 Lysis1.in :homing @16:17:14.20 Air.in :homing @16:17:14.23 Lysis2.in :homing @16:17:14.59 Toroid.jog 626 @16:17:14.68 Plunge.jog 1559 @16:17:14.74 PinPort.jog -107 @16:17:14.80 Lysis1.jog 1380 @16:17:14.89 Lysis2.jog 1392 @16:17:14.98 Air.jog 1382 @16:17:15.67 Archive.setRawPosition! 50 @16:17:15.71 PinPort.setRawPosition! 0 @16:17:15.88 Archive.seek :home @16:17:15.90 PinPort.seek :home @16:17:16.06 PinPort.in :default @16:17:16.20 Archive.in :default @16:17:16.37 Plunge.setRawPosition! 50 @16:17:16.43 Plunge.seek :home @16:17:16.53 Lysis1.setRawPosition! 50 @16:17:16.55 Lysis2.setRawPosition! 50 @16:17:16.64 Lysis1.seek :home @16:17:16.67 Lysis2.seek :home @16:17:16.83 Plunge.in :default @16:17:16.85 Air.setRawPosition! 50 @16:17:16.93 Air.seek :home @16:17:17.04 Lysis2.in :default @16:17:17.07 Lysis1.in :default @16:17:17.19 Toroid.setRawPosition! 0 @16:17:17.26 Toroid.seek :home @16:17:17.36 Toroid.in :default @16:17:17.38 Air.in :default @16:17:17.80 Toroid.seek :clear @16:17:19.41 Carousel.in :default @16:17:19.48 Carousel.home.jog 0 @16:17:19.96 Carousel.in :default @16:17:20.02 Carousel.seek 30 @16:17:20.18 Selecting Cartridge 29 @16:17:20.43 Carousel.seek 29 @16:17:21.29 Lysis1.in :fast @16:17:21.36 Lysis1.seek :locked @16:17:23.96 Plunge.seek :locked @16:17:24.09 Air.in :fast @16:17:24.15 Air.seek :locked @16:17:26.62 Powering cartridge bus @16:17:29.22 Gate.setIR 1750,5000,0,6 @16:17:29.34 Gate.setIR 0,5000,0,6 @16:17:29.40 PuckHeater.configure :default @16:17:29.46 Gate.power :heat,:ON,limit to 5000mA @16:17:30.14 Mounted lysis Cartridge board#Q05037 @16:17:30.17 Using default puck heat loss curve for HeatingAccelInCarousel 29 @16:17:48.13 -> Cmd.startFiltering @16:17:48.37 Preparing to filter cartridge 29 92.07 @16:17:48.64 Priming sample loop w/100ml, bypass w/10ml @16:17:48.66 SamplePump.setPosition! 0ml @16:17:48.71 Bypass.open @16:17:50.16 Intake.open @16:17:50.91 Sea 2.7m deep (18.6psia) @16:17:50.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @16:17:53.51 Vacuum tare is 54% of full scale @16:17:53.64 Pressure.in :default @16:17:53.70 Pressure.hold @16:17:53.88 SamplePump.in :prime @16:17:53.95 SamplePump.seek 10ml,59 seconds @16:18:02.56 Pressure.stop @16:18:02.61 SamplePump.setPosition! 0ml @16:18:02.67 Exhaust.open @16:18:04.12 Bypass.close @16:18:05.61 Pressure.in :default @16:18:05.67 Pressure.hold @16:18:05.79 Sea 2.7m deep (18.7psia) @16:18:05.94 SamplePump.in :prime @16:18:06.01 SamplePump.seek 100ml,5:16 @16:19:38.79 Pressure.stop @16:19:38.84 Bypass.open @16:19:39.48 Intake.close @16:19:40.22 Toroid.in :hold @16:19:40.29 Toroid.seek :crack @16:19:41.79 Toroid.seek :cartridge @16:19:42.89 Toroid.in :default @16:19:42.94 Toroid.seek :clear @16:19:44.90 Pressurizing cartridge to 26psi above 18.2psia @16:19:44.92 SamplePump.setPosition! 0ml @16:19:44.97 Intake.open @16:19:45.63 Bypass.close @16:19:46.28 Exhaust.close @16:19:47.10 Pressure.in :default @16:19:47.16 Pressure.hold @16:19:47.33 SamplePump.in :charge @16:19:47.40 SamplePump.seek 2.3ml,42 seconds @16:19:58.19 Pressure.stop @16:19:58.25 Intake.close @16:19:59.00 Pressurized cartridge with 0.561ml to 26.0psi @16:19:59.01 Isolated @41.3psia (23.1psi) @16:19:59.03 Verifying cartridge holds at least 0.65..0.85 of 41.3psia @16:19:59.06 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @16:20:03.70 Sampling 1000.0ml @16:20:03.72 SamplePump.setPosition! 0ml @16:20:03.78 Exhaust.open @16:20:04.52 Intake.open @16:20:05.39 Pressure.in :default @16:20:05.45 Pressure.hold @16:20:05.56 Sea 3.0m deep (19.2psia) @16:20:05.70 SamplePump.in :sample @16:20:05.77 SamplePump.seek 1000ml,1:23:48 @16:20:08.37 Cartridge 29 filtering 1/1000ml at 0.43ml/s with 25.7psi vacuum 3.3m deep (19.6psia) @16:21:08.37 Cartridge 29 filtering 27/1000ml at 0.44ml/s with 22.9psi vacuum 2.9m deep (18.9psia) @16:22:08.37 Cartridge 29 filtering 52/1000ml at 0.41ml/s with 22.1psi vacuum 1.7m deep (17.2psia) @16:23:08.37 Cartridge 29 filtering 75/1000ml at 0.39ml/s with 25.7psi vacuum 2.4m deep (18.2psia) @16:24:08.38 Cartridge 29 filtering 98/1000ml at 0.38ml/s with 22.2psi vacuum 3.6m deep (20.0psia) @16:25:08.37 Cartridge 29 filtering 119/1000ml at 0.35ml/s with 27.8psi vacuum @16:26:08.38 Cartridge 29 filtering 140/1000ml at 0.36ml/s with 22.1psi vacuum 3.4m deep (19.7psia) @16:27:08.38 Cartridge 29 filtering 160/1000ml at 0.34ml/s with 24.7psi vacuum @16:28:08.38 Cartridge 29 filtering 179/1000ml at 0.33ml/s with 21.6psi vacuum 2.3m deep (18.1psia) @16:29:08.37 Cartridge 29 filtering 197/1000ml at 0.27ml/s with 23.1psi vacuum 2.4m deep (18.2psia) @16:30:08.38 Cartridge 29 filtering 214/1000ml at 0.30ml/s with 21.7psi vacuum 2.6m deep (18.5psia) @16:31:08.37 Cartridge 29 filtering 231/1000ml at 0.29ml/s with 25.5psi vacuum 3.0m deep (19.1psia) @16:32:08.38 Cartridge 29 filtering 247/1000ml at 0.25ml/s with 24.1psi vacuum 2.6m deep (18.5psia) @16:33:08.37 Cartridge 29 filtering 263/1000ml at 0.24ml/s with 23.9psi vacuum 2.3m deep (18.0psia) @16:34:08.38 Cartridge 29 filtering 278/1000ml at 0.25ml/s with 21.9psi vacuum 1.7m deep (17.1psia) @16:35:08.37 Cartridge 29 filtering 292/1000ml at 0.25ml/s with 25.6psi vacuum @16:36:08.38 Cartridge 29 filtering 306/1000ml at 0.21ml/s with 23.2psi vacuum 2.3m deep (18.1psia) @16:37:08.37 Cartridge 29 filtering 319/1000ml at 0.20ml/s with 24.9psi vacuum @16:38:08.38 Cartridge 29 filtering 331/1000ml at 0.20ml/s with 25.0psi vacuum 1.7m deep (17.2psia) @16:39:08.38 Cartridge 29 filtering 344/1000ml at 0.20ml/s with 25.0psi vacuum 2.5m deep (18.4psia) @16:39:39.44 Flow averaged 0.30ml/s over 19:34 @16:39:39.47 Waiting up to 13 seconds for vacuum to reduce from 28.2 to 18.0psi @16:39:40.05 Relieved excess vacuum in .6 seconds @16:39:40.07 SamplePump.seek 1000ml,54:40 @16:40:08.37 Cartridge 29 filtering 356/1000ml at 0.21ml/s with 24.7psi vacuum 2.2m deep (17.9psia) @16:40:10.94 Flow averaged 0.20ml/s over 31 seconds @16:40:10.97 Waiting up to 10 seconds for vacuum to reduce from 26.7 to 18.0psi @16:40:11.55 Relieved excess vacuum in .6 seconds @16:40:11.57 SamplePump.seek 1000ml,54:08 @16:40:26.08 Flow averaged 0.20ml/s over 15 seconds @16:40:26.11 Waiting up to 11 seconds for vacuum to reduce from 27.3 to 18.0psi @16:40:26.69 Relieved excess vacuum in .6 seconds @16:40:26.71 SamplePump.seek 1000ml,53:54 @16:40:38.26 Flow averaged 0.20ml/s over 12 seconds @16:40:38.28 Intake.close @16:40:38.35 Exhaust.close @16:40:38.53 Toroid.in :hold @16:40:38.59 Toroid.seek :bypass @16:40:40.21 Toroid.in :default @16:40:40.28 Toroid.seek :clear @16:40:41.89 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @16:40:41.90 Pressure.stop @16:40:42.51 Sampled 361.4ml @16:40:44.17 -> Cmd.startProcessing 120.0 @16:40:45.62 SPR.command "read_sequences" @16:40:46.02 SPR.command "stats_on" @16:40:46.14 SPR.command "start 0 1" @16:40:46.87 SPR.start /var/log/esp/spr22jun0216h.spr.bz2 @16:40:49.35 Handoff.select :waste @16:40:56.04 Syringe tare is 19% of full scale @16:40:56.07 PinPort.in :locking @16:40:56.15 PinPort.seek :locked @16:40:56.62 Archive.in :fast @16:40:56.67 Lysis2.in :fast @16:40:56.79 Lysis1.seek 1350uL @16:40:56.82 Archive.seek :full @16:40:56.91 Lysis2.seek :full @16:40:58.93 Plunge.seek :reagent @16:40:59.83 Evacuating puck with filter bubble point of 50psi @16:40:59.85 PinPort.seek :locked @16:40:59.95 Handoff.select :waste @16:41:06.53 Air syringe pumping up to 1 stroke to reach 8psi @16:41:06.54 Air stroke #1 of 1 @16:41:06.63 Air.in :pressurize @16:41:06.70 Air.seek :pressurized @16:41:18.27 0.5psi after pressurize @16:41:18.29 Air.in :compress @16:41:18.37 Air.seek :evac @16:41:22.48 0.9psi after compress @16:41:35.05 0.1psi after compress (stable) @16:41:35.07 Air.in :default @16:41:35.14 Air.hold @16:41:35.30 at 0.0psi Opened to waste @16:41:35.31 Handoff.select :blocked @16:41:35.45 Archive syringe pumping up to 8 strokes to reach 50psi @16:41:35.46 Archive stroke #1 of 8 @16:41:35.55 Archive.in :pressurize @16:41:35.61 Archive.seek :pressurized @16:41:48.51 9.1psi after pressurize @16:41:48.53 Archive.in :compress @16:41:48.60 Archive.seek :empty @16:42:03.29 28.5psi after compress @16:42:17.86 33.6psi after compress (stable) @16:42:17.88 Archive.in :gulp @16:42:17.96 Archive.seek :topVent @16:42:25.12 33.8psi after gulp @16:42:37.69 33.7psi after gulp (stable) @16:42:37.70 Archive stroke #2 of 8 @16:42:37.79 Archive.in :pressurize @16:42:37.85 Archive.seek :pressurized @16:42:50.71 33.6psi after pressurize @16:42:50.73 Archive.in :compress @16:42:50.85 Archive.seek :empty @16:43:04.75 Reached 50.2 of 50.0psi after pumping 3443uL (1.99 strokes) @16:43:04.76 Archive.in :default @16:43:04.83 Archive.hold @16:43:04.90 Handoff.select :waste @16:43:13.09 delay 1:00 @16:44:13.12 Handoff.select :blocked @16:44:35.82 Handoff.select :waste @16:44:35.93 2.2psi at evacEnd @16:44:35.94 Handoff.select :blocked @16:44:36.07 Archive.in :fast @16:44:36.14 Archive.seek :topVent @16:44:49.73 Stable @2.9psi after Handoff blocked @16:44:49.75 Archive syringe pumping up to 8 strokes to reach 33psi @16:44:49.77 Archive stroke #1 of 8 @16:44:49.91 Archive.in :pressurize @16:44:49.98 Archive.seek :pressurized @16:45:02.87 4.6psi after pressurize @16:45:02.88 Archive.in :compress @16:45:02.96 Archive.seek :empty @16:45:17.64 8.1psi after compress @16:46:00.21 32.4psi after compress (stable) @16:46:00.23 Archive.in :gulp @16:46:00.31 Archive.seek :topVent @16:46:01.30 Reached 33.0 of 32.9psi after pumping 1730uL (1.00 strokes) @16:46:01.31 Archive.in :default @16:46:01.38 Archive.hold @16:46:02.12 Handoff.select :waste @16:46:14.85 delay 1:00 @16:47:14.86 Handoff.select :blocked @16:47:37.55 Handoff.select :waste @16:47:38.05 2.5psi at evacEnd @16:47:38.07 Air.seek :fullVent @16:47:39.49 Archive.in :fast @16:47:39.55 Archive.seek :topVent @16:47:45.95 Seawater evacuated, Presure above/below filter released. Lysis begins @16:47:46.04 Lysis1.in :slow @16:47:46.11 Lysis1.seek 1050uL @16:48:16.12 delay 5 seconds @16:48:21.14 Handoff.select :blocked @16:48:21.31 Lysis1.seek 450uL @16:49:21.26 delay 3 seconds @16:49:24.33 Air.in mix with 1.40048 parts Lysis1 @16:49:24.76 Air.seek :evac @16:49:24.81 Lysis1.seek 300uL @16:49:39.99 Lysis1.seek :empty @16:50:10.07 Lysis buffer delivered @27.2psi @16:50:10.08 Polling Can every 2:00 @16:50:10.63 PuckHeater.configure :default #ambient 16.4C @16:50:10.68 PuckHeater.seek 80C @16:51:07.39 PuckHeater.hold 2:00,80C @16:53:07.06 Polling Can every 2:00 @16:53:07.61 PuckHeater.configure :default #ambient 16.5C @16:53:07.67 PuckHeater.hold 30 seconds,79C @16:53:37.74 Polling Can every 2:00 @16:53:38.30 PuckHeater.configure :default #ambient 16.5C @16:53:38.38 PuckHeater.hold 30 seconds,78.5C @16:54:08.46 Polling Can every 2:00 @16:54:09.00 PuckHeater.configure :default #ambient 16.5C @16:54:09.05 PuckHeater.hold 10:00,77.9C @17:04:06.92 Lysis complete. Syringe pressure @37.1psi @17:04:06.93 Handoff.select :blocked @17:04:06.98 Archive syringe pumping up to 5 strokes to reach 40psi @17:04:06.99 Archive stroke #1 of 5 @17:04:07.08 Archive.in :pressurize @17:04:07.15 Archive.seek :pressurized @17:04:20.01 36.9psi after pressurize @17:04:20.03 Archive.in :compress @17:04:20.10 Archive.seek :empty @17:04:28.68 Reached 41.4 of 40.0psi after pumping 1599uL (0.92 strokes) @17:04:28.69 Archive.in :default @17:04:28.76 Archive.hold @17:04:28.83 delay 2 seconds @17:04:30.85 Archive.in :fast @17:04:30.94 Archive.seek :full @17:04:37.95 Lysis1.seek 138uL @17:04:51.82 delay 1 second @17:04:52.85 Archive.seek :full @17:04:52.89 Lysis1.seek :empty @17:05:06.88 Initial air pressure @55.2psi @17:05:06.96 Lysis1.in :extract @17:05:07.03 Lysis1.seek 600uL,nil @17:05:10.51 Archive syringe pumping up to 6 strokes to reach 25psi @17:05:10.52 Archive stroke #1 of 6 @17:05:10.62 Archive.in :pressurize @17:05:10.72 Archive.seek :pressurized @17:05:23.61 15.9psi after pressurize @17:05:23.63 Archive.in :compress @17:05:23.70 Archive.seek :empty @17:05:36.74 Archive.in :default @17:05:36.79 Archive.hold @17:05:36.95 Initial 600uL lysate @18.5psi @17:05:36.96 Handoff.select :blocked @17:05:37.06 Initial air pressure @19.8psi @17:05:37.14 Lysis1.seek 825uL,nil @17:05:38.41 Archive syringe pumping up to 6 strokes to reach 17psi @17:05:38.42 Archive stroke #1 of 6 @17:05:38.57 Archive.in :compress @17:05:38.63 Archive.seek :empty @17:05:40.28 15.9psi after compress @17:05:43.80 Archive.in :default @17:05:43.85 Archive.hold @17:05:44.04 Recovered 825uL lysate @16.5psi @17:05:44.06 Air.in :halfSpeed @17:05:44.13 Air.seek :fullVent @17:05:46.34 Puck Top Vented, bottom @0.4psi @17:05:46.35 Handoff.select :waste @17:05:46.47 delay 5 seconds @17:05:51.53 Handoff open to waste, bottom @-0.2psi @17:05:51.55 Air.in :default @17:05:51.62 Air.seek :evac @17:05:53.04 Lysis1.in :DAmix @17:05:53.15 Lysis2.in :DAmix @17:05:53.56 Lysis1.seek 575uL @17:05:53.60 Lysis2.seek 1480uL @17:06:28.65 delay 5 seconds @17:06:33.67 Handoff.select SPR @17:06:33.79 SPR.command "start 2 14" @17:06:34.35 SPR stabilized @17:06:34.49 delay 10 seconds @17:06:44.50 SPR.command "start 1 0" @17:06:47.71 Waiting up to 4:00 for SPR to enter state 1 @17:08:50.09 SPR.command "hold_on" @17:08:51.50 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:08:51.97 Lysis1.seek 275uL @17:08:52.01 Lysis2.seek 1180uL @17:09:34.05 delay 15 seconds @17:09:49.07 SPR.command "hold_off" @17:09:52.15 Waiting up to 38:00 for SPR to enter state 7 @17:30:41.98 SPRsummary:-52.34,169.331,170.605,220.453 after 3139s @17:37:19.21 SPR analysis completed @17:37:24.32 Ended log spr22jun0216h.spr.bz2 @17:37:24.33 SPR.command "stats_off" @17:37:25.97 -> Cmd.stop @17:37:26.07 Handoff.select :close @17:37:26.20 Gate.power :heat,:OFF @17:37:26.21 Gate.power :cartridge,:OFF @17:37:26.30 Carousel.seek 29 @17:37:26.47 Plunge.seek :locked @17:37:27.33 PinPort.in :unlocking @17:37:27.39 PinPort.seek :clear @17:37:27.94 Archive.in :unlocking @17:37:28.07 Archive.seek :clear @17:37:28.18 Air.in :unlocking @17:37:28.27 Air.seek :clear @17:37:28.38 Lysis1.in :unlocking @17:37:28.47 Lysis1.seek :clear @17:37:28.51 Lysis2.in :unlocking @17:37:28.62 Lysis2.seek :clear @17:37:32.19 Gate.power :motor,:OFF @17:37:32.20 Gate.power :core,:OFF @17:37:32.23 Gate.power :analytic1,:OFF @17:37:32.25 Gate.power :analytic2,:OFF @17:37:32.27 Gate.power :analytic3,:OFF @17:37:32.30 Gate.power :main,:OFF @17:37:32.32 Safely stopped and ready to power off :STOPPED