@13:44:58.26PDT06-Jun-22
>BREAK< ESP3 Gateway v1.12 for core bus @13:44:58.28 >BREAK< ESP3 Gateway v1.12 for cartridge bus @13:45:00.96 Configuration for honu by methane@mbari.org 3/16/22 /home/esp/esp2/type/auv/honu/configure.rb @13:45:11.09 Polling Can every 2:00 @13:45:12.60 Clients may connect on TCP port 9999 @13:45:12.66 Connecting to 10.89.5.2:9999 @13:45:12.73 Waiting for espclients... @13:45:13.01 Session Begun @13:45:14.84 -> showStatus true @13:45:16.86 -> Cmd.loadCartridge -10 @13:45:16.94 Gate.power :main,:ON,limit to 5000mA @13:45:16.96 Gate.power :analytic1,:ON,limit to 5000mA @13:45:16.98 Gate.power :analytic2,:ON,limit to 5000mA @13:45:17.01 Gate.power :analytic3,:ON,limit to 5000mA @13:45:17.20 Powering core bus @13:45:19.41 SPR Module 1, FW Rev. B, FPGA Rev. C @13:45:19.91 Archive.configure :default @13:45:20.04 Air.configure :default @13:45:20.14 Lysis1.configure :default @13:45:20.26 Lysis2.configure :default @13:45:20.38 Plunge.configure :default @13:45:20.52 SamplePump.configure :default @13:45:20.65 Toroid.configure :default @13:45:20.77 Carousel.configure :default @13:45:20.90 Pressure.configure :default @13:45:21.03 ExtraPump.configure :default @13:45:21.14 PinPort.configure :default @13:45:21.20 All elf controllers running firmware version 4.79 @13:45:21.22 Gate.power :motor,:ON,limit to 5000mA @13:45:21.33 Archive.in :default @13:45:21.60 Archive.home.jog 0 @13:45:21.86 Toroid.in :default @13:45:21.94 Lysis2.in :default @13:45:21.99 Air.in :default @13:45:22.03 PinPort.in :default @13:45:22.07 Lysis1.in :default @13:45:22.11 Plunge.in :default @13:45:22.33 Toroid.home.jog 0 @13:45:22.38 Lysis2.home.jog 0 @13:45:22.41 Air.home.jog 0 @13:45:22.43 PinPort.home.jog 0 @13:45:22.46 Plunge.home.jog 0 @13:45:22.53 Lysis1.home.jog 0 @13:45:23.20 Archive.in :homing @13:45:23.43 Archive.jog 1388 @13:45:23.92 PinPort.in :homing @13:45:24.00 Plunge.in :homing @13:45:24.10 Lysis2.in :homing @13:45:24.21 Air.in :homing @13:45:24.31 Toroid.in :homing @13:45:24.36 Lysis1.in :homing @13:45:24.54 PinPort.jog -108 @13:45:24.57 Lysis2.jog 1390 @13:45:24.62 Plunge.jog 1554 @13:45:24.65 Air.jog 1387 @13:45:24.69 Toroid.jog 634 @13:45:24.75 Lysis1.jog 1379 @13:45:24.97 PinPort.setRawPosition! 0 @13:45:24.98 Archive.setRawPosition! 50 @13:45:25.05 PinPort.seek :home @13:45:25.09 Archive.seek :home @13:45:25.22 PinPort.in :default @13:45:25.42 Archive.in :default @13:45:25.90 Lysis2.setRawPosition! 50 @13:45:25.96 Lysis2.seek :home @13:45:26.08 Plunge.setRawPosition! 50 @13:45:26.10 Lysis1.setRawPosition! 50 @13:45:26.12 Air.setRawPosition! 50 @13:45:26.21 Plunge.seek :home @13:45:26.28 Air.seek :home @13:45:26.32 Lysis1.seek :home @13:45:26.49 Lysis2.in :default @13:45:26.61 Plunge.in :default @13:45:27.12 Lysis1.in :default @13:45:27.19 Air.in :default @13:45:27.32 Toroid.setRawPosition! 0 @13:45:27.37 Toroid.seek :home @13:45:27.47 Toroid.in :default @13:45:27.61 Toroid.seek :clear @13:45:29.22 Carousel.in :default @13:45:29.29 Carousel.home.jog 0 @13:45:29.77 Carousel.in :default @13:45:29.84 Carousel.seek 20 @13:45:30.00 Selecting Cartridge 19 @13:45:30.26 Carousel.seek 19 @13:45:30.74 Lysis1.in :fast @13:45:30.81 Lysis1.seek :locked @13:45:33.39 Plunge.seek :locked @13:45:33.53 Air.in :fast @13:45:33.59 Air.seek :locked @13:45:36.05 Powering cartridge bus @13:45:38.66 Gate.setIR 1750,5000,0,6 @13:45:38.78 Gate.setIR 0,5000,0,6 @13:45:38.85 PuckHeater.configure :default @13:45:38.90 Gate.power :heat,:ON,limit to 5000mA @13:45:39.57 Mounted lysis Cartridge board#Q05038 @13:45:39.60 Using default puck heat loss curve for HeatingAccelInCarousel 19 @13:45:57.26 -> Cmd.startFiltering 92.07@13:45:57.51 Preparing to filter cartridge 19 92.07@13:45:57.51 Preparing to filter cartridge 19 @13:45:57.78 Priming sample loop w/100ml, bypass w/10ml @13:45:57.79 SamplePump.setPosition! 0ml @13:45:57.83 Bypass.open @13:45:59.28 Intake.open @13:46:00.03 Sea 14.1m deep (35.6psia) @13:46:00.05 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @13:46:02.62 Vacuum tare is 54% of full scale @13:46:02.75 Intake.close @13:46:02.81 Bypass.close @13:46:03.55 Verifying toroid holds at least 0.6..0.85 of 35.5psia @13:46:03.56 Waiting up to 40 seconds for toroid pressure to stabilize +/-1.50psi @13:46:08.14 Toroid pressure rose 2% to 36.2psia @13:46:08.15 Bypass.open @13:46:08.84 Intake.open @13:46:10.24 Pressure.in :default @13:46:10.30 Pressure.hold @13:46:10.48 SamplePump.in :prime @13:46:10.54 SamplePump.seek 10ml,59 seconds @13:46:19.16 Pressure.stop @13:46:19.21 SamplePump.setPosition! 0ml @13:46:19.26 Exhaust.open @13:46:20.75 Bypass.close @13:46:22.25 Pressure.in :default @13:46:22.31 Pressure.hold @13:46:22.42 Sea 14.6m deep (36.3psia) @13:46:22.56 SamplePump.in :prime @13:46:22.62 SamplePump.seek 100ml,5:16 @13:47:55.60 Pressure.stop @13:47:55.65 Bypass.open @13:47:56.32 Intake.close @13:47:57.09 Toroid.in :hold @13:47:57.15 Toroid.seek :crack @13:47:58.66 Toroid.seek :cartridge @13:47:59.75 Toroid.in :default @13:47:59.81 Toroid.seek :clear @13:48:01.77 Exhaust.close @13:48:01.81 Bypass.close @13:48:02.56 Verifying cartridge holds at least 0.65..0.85 of 36.8psia @13:48:02.58 Waiting up to 40 seconds for cartridge pressure to stabilize +/-1.50psi @13:48:07.20 Sampling 1000.0ml @13:48:07.22 SamplePump.setPosition! 0ml @13:48:07.28 Exhaust.open @13:48:08.00 Intake.open @13:48:08.84 Pressure.in :default @13:48:08.90 Pressure.hold @13:48:09.02 Sea 14.9m deep (36.7psia) @13:48:09.16 SamplePump.in :sample @13:48:09.22 SamplePump.seek 1000ml,1:23:48 @13:48:11.87 Cartridge 19 filtering 1/1000ml at 0.43ml/s with 23.2psi vacuum 14.1m deep (35.6psia) @13:49:11.86 Cartridge 19 filtering 26/1000ml at 0.42ml/s with 27.8psi vacuum 13.0m deep (33.9psia) @13:50:11.86 Cartridge 19 filtering 51/1000ml at 0.39ml/s with 26.6psi vacuum 13.2m deep (34.2psia) @13:51:11.86 Cartridge 19 filtering 74/1000ml at 0.37ml/s with 28.2psi vacuum 13.4m deep (34.5psia) @13:52:11.87 Cartridge 19 filtering 96/1000ml at 0.37ml/s with 26.4psi vacuum 15.0m deep (36.8psia) @13:53:11.86 Cartridge 19 filtering 118/1000ml at 0.38ml/s with 22.1psi vacuum 15.9m deep (38.1psia) @13:54:11.87 Cartridge 19 filtering 139/1000ml at 0.33ml/s with 23.3psi vacuum 13.8m deep (35.1psia) @13:55:11.86 Cartridge 19 filtering 159/1000ml at 0.31ml/s with 24.7psi vacuum 13.7m deep (35.0psia) @13:56:11.87 Cartridge 19 filtering 179/1000ml at 0.30ml/s with 27.1psi vacuum 13.7m deep (34.9psia) @13:57:11.86 Cartridge 19 filtering 198/1000ml at 0.30ml/s with 23.1psi vacuum 13.9m deep (35.2psia) @13:58:11.87 Cartridge 19 filtering 216/1000ml at 0.32ml/s with 22.7psi vacuum 14.4m deep (36.0psia) @13:59:11.86 Cartridge 19 filtering 234/1000ml at 0.27ml/s with 23.9psi vacuum 13.9m deep (35.2psia) @14:00:11.87 Cartridge 19 filtering 252/1000ml at 0.26ml/s with 24.6psi vacuum 13.0m deep (33.8psia) @14:01:11.86 Cartridge 19 filtering 269/1000ml at 0.28ml/s with 20.5psi vacuum 15.2m deep (37.2psia) @14:02:11.87 Cartridge 19 filtering 285/1000ml at 0.27ml/s with 22.8psi vacuum 13.7m deep (34.9psia) @14:03:11.86 Cartridge 19 filtering 301/1000ml at 0.25ml/s with 24.4psi vacuum 15.3m deep (37.3psia) @14:04:11.92 Cartridge 19 filtering 316/1000ml at 0.24ml/s with 23.1psi vacuum 13.5m deep (34.6psia) @14:05:11.86 Cartridge 19 filtering 331/1000ml at 0.24ml/s with 24.4psi vacuum 13.6m deep (34.8psia) @14:06:11.87 Cartridge 19 filtering 346/1000ml at 0.22ml/s with 24.5psi vacuum 13.8m deep (35.0psia) @14:07:11.86 Cartridge 19 filtering 361/1000ml at 0.24ml/s with 24.5psi vacuum 14.7m deep (36.4psia) @14:08:11.87 Cartridge 19 filtering 375/1000ml at 0.25ml/s with 22.7psi vacuum 14.1m deep (35.6psia) @14:09:11.87 Cartridge 19 filtering 388/1000ml at 0.21ml/s with 24.0psi vacuum 14.2m deep (35.6psia) @14:10:11.87 Cartridge 19 filtering 402/1000ml at 0.22ml/s with 24.7psi vacuum 13.4m deep (34.5psia) @14:11:11.87 Cartridge 19 filtering 415/1000ml at 0.22ml/s with 24.1psi vacuum 12.9m deep (33.8psia) @14:12:11.86 Cartridge 19 filtering 427/1000ml at 0.20ml/s with 24.1psi vacuum 13.8m deep (35.1psia) @14:13:11.86 Cartridge 19 filtering 440/1000ml at 0.21ml/s with 23.3psi vacuum 12.8m deep (33.6psia) @14:14:12.33 Cartridge 19 filtering 452/1000ml at 0.20ml/s with 24.7psi vacuum 13.9m deep (35.2psia) @14:15:11.86 Cartridge 19 filtering 464/1000ml at 0.20ml/s with 23.8psi vacuum 12.7m deep (33.5psia) @14:16:11.92 Cartridge 19 filtering 477/1000ml at 0.20ml/s with 26.0psi vacuum 13.0m deep (33.9psia) @14:16:35.18 Flow averaged 0.28ml/s over 28:27 @14:16:35.22 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @14:16:35.80 Relieved excess vacuum in .6 seconds @14:16:35.82 SamplePump.seek 1000ml,43:42 @14:17:11.86 Cartridge 19 filtering 489/1000ml at 0.20ml/s with 24.2psi vacuum 12.7m deep (33.4psia) @14:18:11.87 Cartridge 19 filtering 501/1000ml at 0.21ml/s with 23.9psi vacuum 13.7m deep (35.0psia) @14:18:29.14 Flow averaged 0.20ml/s over 1:54 @14:18:29.17 Waiting up to 11 seconds for vacuum to reduce from 27.1 to 18.0psi @14:18:29.74 Relieved excess vacuum in .6 seconds @14:18:29.77 SamplePump.seek 1000ml,41:47 @14:18:33.27 Flow estimate reduced to 0.16ml/s @14:18:33.29 Intake.close @14:18:33.35 Exhaust.close @14:18:33.54 Toroid.in :hold @14:18:33.60 Toroid.seek :bypass @14:18:35.22 Toroid.in :default @14:18:35.29 Toroid.seek :clear @14:18:36.90 Cartridge::Sampler::Clogged in FILTERING -- Cartridge clogged @14:18:36.92 Pressure.stop @14:18:37.10 Sampled 505.1ml @14:18:39.23 -> Cmd.startProcessing 120.0 @14:18:40.68 SPR.command "read_sequences" @14:18:40.72 SPR.command "stats_on" @14:18:40.82 SPR.command "start 0 1" @14:18:41.56 SPR.start /var/log/esp/spr22jun0614h.spr.bz2 @14:18:43.52 Handoff.select :waste @14:18:50.20 Syringe tare is 19% of full scale @14:18:50.22 PinPort.in :locking @14:18:50.29 PinPort.seek :locked @14:18:50.77 Archive.in :fast @14:18:50.83 Lysis2.in :fast @14:18:50.89 Lysis1.seek 1350uL @14:18:51.01 Archive.seek :full @14:18:51.06 Lysis2.seek :full @14:18:53.04 Plunge.seek :reagent @14:18:53.91 Evacuating puck with filter bubble point of 50psi @14:18:53.94 PinPort.seek :locked @14:18:54.03 Handoff.select :waste @14:19:00.61 Air syringe pumping up to 1 stroke to reach 8psi @14:19:00.62 Air stroke #1 of 1 @14:19:00.71 Air.in :pressurize @14:19:00.78 Air.seek :pressurized @14:19:12.39 0.2psi after pressurize @14:19:12.41 Air.in :compress @14:19:12.48 Air.seek :evac @14:19:16.56 0.4psi after compress @14:19:29.13 0.1psi after compress (stable) @14:19:29.15 Air.in :default @14:19:29.22 Air.hold @14:19:29.43 at 0.0psi Opened to waste @14:19:29.44 Handoff.select :blocked @14:19:29.58 Archive syringe pumping up to 8 strokes to reach 50psi @14:19:29.59 Archive stroke #1 of 8 @14:19:29.67 Archive.in :pressurize @14:19:29.74 Archive.seek :pressurized @14:19:42.59 9.8psi after pressurize @14:19:42.63 Archive.in :compress @14:19:42.72 Archive.seek :empty @14:19:57.40 27.2psi after compress @14:20:15.97 37.5psi after compress (stable) @14:20:15.99 Archive.in :gulp @14:20:16.07 Archive.seek :topVent @14:20:23.23 38.2psi after gulp @14:20:35.80 37.9psi after gulp (stable) @14:20:35.81 Archive stroke #2 of 8 @14:20:35.91 Archive.in :pressurize @14:20:35.98 Archive.seek :pressurized @14:20:48.85 37.3psi after pressurize @14:20:48.89 Archive.in :compress @14:20:48.96 Archive.seek :empty @14:21:02.77 Reached 50.3 of 50.0psi after pumping 3442uL (1.99 strokes) @14:21:02.79 Archive.in :default @14:21:02.86 Archive.hold @14:21:02.93 Handoff.select :waste @14:21:13.11 delay 1:00 @14:22:13.13 Handoff.select :blocked @14:22:53.81 Handoff.select :waste @14:22:53.93 6.7psi at evacEnd @14:22:53.94 Handoff.select :blocked @14:22:54.07 Archive.in :fast @14:22:54.13 Archive.seek :topVent @14:23:07.73 Stable @6.8psi after Handoff blocked @14:23:07.75 Archive syringe pumping up to 8 strokes to reach 37psi @14:23:07.76 Archive stroke #1 of 8 @14:23:07.88 Archive.in :pressurize @14:23:07.96 Archive.seek :pressurized @14:23:20.81 8.3psi after pressurize @14:23:20.82 Archive.in :compress @14:23:20.90 Archive.seek :empty @14:23:35.58 11.3psi after compress @14:24:24.65 29.0psi after compress (stable) @14:24:24.67 Archive.in :gulp @14:24:24.74 Archive.seek :topVent @14:24:31.90 30.1psi after gulp @14:24:44.47 30.9psi after gulp (stable) @14:24:44.48 Archive stroke #2 of 8 @14:24:44.57 Archive.in :pressurize @14:24:44.64 Archive.seek :pressurized @14:24:57.49 31.8psi after pressurize @14:24:57.51 Archive.in :compress @14:24:57.61 Archive.seek :empty @14:25:12.29 34.0psi after compress @14:25:16.38 Reached 36.8 of 36.8psi after pumping 3460uL (2.00 strokes) @14:25:16.39 Archive.in :default @14:25:16.47 Archive.hold @14:25:16.56 Handoff.select :waste @14:25:34.24 delay 1:00 @14:26:34.25 Handoff.select :blocked @14:26:56.93 Handoff.select :waste @14:26:57.04 2.2psi at evacEnd @14:26:57.06 Air.seek :fullVent @14:26:58.48 Archive.in :fast @14:26:58.54 Archive.seek :topVent @14:27:05.63 Seawater evacuated, Presure above/below filter released. Lysis begins @14:27:05.72 Lysis1.in :slow @14:27:05.78 Lysis1.seek 1050uL @14:27:35.79 delay 5 seconds @14:27:40.81 Handoff.select :blocked @14:27:40.98 Lysis1.seek 450uL @14:28:40.91 delay 3 seconds @14:28:43.98 Air.in mix with 1.40048 parts Lysis1 @14:28:44.46 Air.seek :evac @14:28:44.52 Lysis1.seek 300uL @14:28:59.63 Lysis1.seek :empty @14:29:29.72 Lysis buffer delivered @15.3psi @14:29:29.74 Polling Can every 2:00 @14:29:30.28 PuckHeater.configure :default #ambient 16.1C @14:29:30.33 PuckHeater.seek 80C @14:30:37.49 PuckHeater.hold 2:00,80C @14:32:37.32 Polling Can every 2:00 @14:32:37.86 PuckHeater.configure :default #ambient 16.1C @14:32:37.91 PuckHeater.hold 30 seconds,79C @14:33:08.03 Polling Can every 2:00 @14:33:08.57 PuckHeater.configure :default #ambient 16.1C @14:33:08.62 PuckHeater.hold 30 seconds,78.5C @14:33:38.75 Polling Can every 2:00 @14:33:39.30 PuckHeater.configure :default #ambient 16.1C @14:33:39.36 PuckHeater.hold 10:00,78C @14:43:38.12 Lysis complete. Syringe pressure @17.5psi @14:43:38.13 Handoff.select :blocked @14:43:38.18 Archive syringe pumping up to 5 strokes to reach 40psi @14:43:38.19 Archive stroke #1 of 5 @14:43:38.28 Archive.in :pressurize @14:43:38.35 Archive.seek :pressurized @14:43:51.21 17.2psi after pressurize @14:43:51.23 Archive.in :compress @14:43:51.31 Archive.seek :empty @14:44:00.03 Reached 40.6 of 40.0psi after pumping 1602uL (0.93 strokes) @14:44:00.05 Archive.in :default @14:44:00.12 Archive.hold @14:44:00.22 delay 2 seconds @14:44:02.24 Archive.in :fast @14:44:02.30 Archive.seek :full @14:44:09.02 Lysis1.seek 138uL @14:44:22.88 delay 1 second @14:44:23.91 Archive.seek :full @14:44:23.95 Lysis1.seek :empty @14:44:37.92 Initial air pressure @50.4psi @14:44:38.02 Lysis1.in :extract @14:44:38.11 Lysis1.seek 600uL,nil @14:44:40.94 Archive syringe pumping up to 6 strokes to reach 25psi @14:44:40.95 Archive stroke #1 of 6 @14:44:41.05 Archive.in :pressurize @14:44:41.13 Archive.seek :pressurized @14:44:54.00 15.8psi after pressurize @14:44:54.02 Archive.in :compress @14:44:54.12 Archive.seek :empty @14:45:08.80 15.9psi after compress @14:45:09.43 Archive.in :default @14:45:09.48 Archive.hold @14:45:09.63 Initial 600uL lysate @20.9psi @14:45:09.64 Handoff.select :blocked @14:45:09.74 Initial air pressure @21.8psi @14:45:09.81 Lysis1.seek 825uL,nil @14:45:11.00 Archive syringe pumping up to 6 strokes to reach 17psi @14:45:11.01 Archive stroke #1 of 6 @14:45:11.12 Archive.in :compress @14:45:11.20 Archive.seek :empty @14:45:11.44 14.5psi after compress @14:45:24.01 15.8psi after compress (stable) @14:45:24.11 Archive.in :default @14:45:24.16 Archive.hold @14:45:24.39 Recovered 825uL lysate @16.0psi @14:45:24.40 Air.in :halfSpeed @14:45:24.48 Air.seek :fullVent @14:45:26.68 Puck Top Vented, bottom @0.9psi @14:45:26.70 Handoff.select :waste @14:45:26.83 delay 5 seconds @14:45:31.91 Handoff open to waste, bottom @-0.0psi @14:45:31.93 Air.in :default @14:45:32.01 Air.seek :evac @14:45:33.43 Lysis2.in :DAmix @14:45:33.55 Lysis1.in :DAmix @14:45:34.07 Lysis2.seek 1480uL @14:45:34.11 Lysis1.seek 575uL @14:46:09.17 delay 5 seconds @14:46:14.19 Handoff.select SPR @14:46:14.31 SPR.command "start 2 14" @14:46:14.94 SPR stabilized @14:46:15.08 delay 10 seconds @14:46:25.10 SPR.command "start 1 0" @14:46:28.30 Waiting up to 4:00 for SPR to enter state 1 @14:48:30.67 SPR.command "hold_on" @14:48:32.09 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:48:32.60 Lysis2.seek 1180uL @14:48:32.68 Lysis1.seek 275uL @14:49:14.64 delay 15 seconds @14:49:29.66 SPR.command "hold_off" @14:49:33.23 Waiting up to 38:00 for SPR to enter state 7 @15:10:23.51 SPRsummary:0.384,117.384,123.064,152.263 after 3252s @15:17:00.74 SPR analysis completed @15:17:06.69 Ended log spr22jun0614h.spr.bz2 @15:17:06.71 SPR.command "stats_off" @15:17:08.03 -> Cmd.stop @15:17:08.13 Handoff.select :close @15:17:08.26 Gate.power :heat,:OFF @15:17:08.27 Gate.power :cartridge,:OFF @15:17:08.36 Carousel.seek 19 @15:17:08.55 Plunge.seek :locked @15:17:09.40 PinPort.in :unlocking @15:17:09.47 PinPort.seek :clear @15:17:10.01 Archive.in :unlocking @15:17:10.14 Archive.seek :clear @15:17:10.23 Air.in :unlocking @15:17:10.35 Lysis1.in :unlocking @15:17:10.39 Air.seek :clear @15:17:10.55 Lysis1.seek :clear @15:17:10.59 Lysis2.in :unlocking @15:17:10.70 Lysis2.seek :clear @15:17:14.29 Gate.power :motor,:OFF @15:17:14.31 Gate.power :core,:OFF @15:17:14.34 Gate.power :analytic1,:OFF @15:17:14.36 Gate.power :analytic2,:OFF @15:17:14.39 Gate.power :analytic3,:OFF @15:17:14.41 Gate.power :main,:OFF @15:17:14.43 Safely stopped and ready to power off :STOPPED