@12:05:47.01PDT17-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:05:48.81 >BREAK< ESP3 Gateway v1.43 for core bus @12:05:48.84 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:05:52.49 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @12:05:53.19 Sea pressure (Abs) tare is 6% of full scale @12:05:56.87 Polling Can every 2:00 @12:05:56.99 Loading /home/esp/esp2/bin/processAll mission @12:05:57.49 Sea pressure (Abs) tare is 6% of full scale @12:05:57.52 Sampling default goal volumes @12:05:57.56 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @12:05:57.94 Gate.power :sampler,:OFF,limit to 3500mA @12:05:57.96 Gate.power :skimmer,:OFF,limit to 3500mA @12:05:58.00 Gate.power :analytic1,:ON,limit to 4000mA @12:05:59.14 Plunge.configure :default @12:05:59.36 SamplePump.configure :default @12:05:59.43 SPR Module 1, FW Rev. B, FPGA Rev. C @12:05:59.54
Toroid.configure :default @12:05:59.68 Carousel.configure :default @12:05:59.73 Carousel.hold @12:05:59.80 Carousel.configure :default @12:05:59.94 Abs.configure :default @12:06:00.07 PhaseSeparator.configure :default @12:06:00.21 Archive.configure :default @12:06:00.34 Air.configure :default @12:06:00.46 Lysis1.configure :default @12:06:00.57 Lysis2.configure :default @12:06:00.73 PinPort.configure :default @12:06:00.78 All elf controllers running firmware version 4.92 @12:06:01.04 Plunge.in :default @12:06:01.42 Plunge.home.jog 0 @12:06:01.63 PinPort.in :default @12:06:01.70 Lysis2.in :default @12:06:01.73 Air.in :default @12:06:01.76 Archive.in :default @12:06:01.78 Toroid.in :default @12:06:01.83 Lysis1.in :default @12:06:02.04 PinPort.home.jog 0 @12:06:02.12 Air.home.jog 0 @12:06:02.14 Lysis2.home.jog 0 @12:06:02.17 Lysis1.home.jog 0 @12:06:02.19 Toroid.home.jog 0 @12:06:02.22 Archive.home.jog 0 @12:06:03.78 PinPort.in :homing @12:06:03.86 Lysis2.in :homing @12:06:03.89 Air.in :homing @12:06:03.91 Toroid.in :homing @12:06:03.93 Archive.in :homing @12:06:03.96 Plunge.in :homing @12:06:03.99 Lysis1.in :homing @12:06:04.20 PinPort.jog -107 @12:06:04.31 Air.jog 1374 @12:06:04.35 Lysis2.jog 1404 @12:06:04.39 Toroid.jog 628 @12:06:04.85 Plunge.jog 162 @12:06:04.91 Archive.jog 1399 @12:06:04.94 Lysis1.jog 1397 @12:06:05.38 PinPort.setRawPosition! 0 @12:06:05.43 PinPort.seek :home @12:06:05.52 PinPort.in :default @12:06:05.63 Plunge.setRawPosition! 50 @12:06:05.69 Plunge.seek :home @12:06:05.97 Plunge.in :default @12:06:06.22 Air.setRawPosition! 50 @12:06:06.31 Lysis2.setRawPosition! 50 @12:06:06.35 Air.seek :home @12:06:06.46 Lysis2.seek :home @12:06:06.67 Archive.setRawPosition! 50 @12:06:06.72 Lysis1.setRawPosition! 50 @12:06:06.81 Lysis1.seek :home @12:06:06.87 Archive.seek :home @12:06:07.08 Air.in :default @12:06:07.10 Lysis2.in :default @12:06:07.28 Archive.in :default @12:06:07.32 Lysis1.in :default @12:06:07.46 Toroid.setRawPosition! 0 @12:06:07.52 Toroid.seek :home @12:06:07.62 Toroid.in :default @12:06:07.85 Toroid.seek :clear @12:06:08.69 Session Begun @12:06:09.72
Carousel.in :default @12:06:09.89 Carousel.home.seek 58 @12:06:10.18 Selecting Cartridge 58 @12:06:10.51 Carousel.seek 58 @12:06:10.82 Lysis1.in :fast @12:06:10.91 Lysis1.seek :locked @12:06:13.57 Plunge.seek :locked @12:06:13.78 Air.in :fast @12:06:13.86 Air.seek :locked @12:06:14.86
Carousel.reconfigure :locked @12:06:15.03 Powering cartridge bus @12:06:17.70 Gate.setIR 1750,5000,0,6 @12:06:17.82 Gate.setIR 0,5000,0,6 @12:06:17.91 PuckHeater.configure :default @12:06:18.00 Gate.power :heat,:ON,limit to 5000mA @12:06:18.73 Mounted lysis Cartridge @12:06:18.76 WARNING: Using default puck temperature sensor calibration @12:06:18.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:06:20.40 SPR.command "read_sequences" @12:06:21.09 SPR.command "stats_on" @12:06:21.75 SPR.command "start 0 1" @12:06:21.97 SPR.start /var/log/esp/spr24sep1712h.spr.bz2 @12:06:24.45 Waiting for baseline SPR stabilization @12:06:24.46 Handoff.select :waste @12:06:24.65 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:06:31.29 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:06:31.32 PinPort.in :locking @12:06:31.43 PinPort.seek :locked @12:06:31.95 Archive.in :fast @12:06:32.09 Lysis2.in :fast @12:06:32.15 Lysis1.seek 1100uL @12:06:32.24 Archive.seek :full @12:06:32.43 Lysis2.seek 1100uL @12:06:36.75 delayUntil "12:16:24.41" @12:16:24.43 Lysis2.in :DAmix @12:16:24.57 Lysis1.in :DAmix @12:16:25.16 Lysis2.seek 950uL @12:16:25.26 Lysis1.seek 950uL @12:16:46.07 delay 5 seconds @12:16:51.09 Handoff.select SPR @12:16:51.25 SPR.command "start 2 14" @12:16:51.97 SPR stabilized @12:16:52.16 delay 10 seconds @12:17:02.18 SPR.command "start 2 0" @12:17:05.34 Waiting up to 11:00 for SPR to enter state 1 @12:19:03.00 SPR.command "hold_on" @12:19:04.40 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @12:19:05.09 Lysis2.seek 500uL @12:19:05.13 Lysis1.seek 500uL @12:20:07.33 delay 15 seconds @12:20:22.37 SPR.command "hold_off" @12:20:24.91 Waiting up to 1:00:00 for SPR to enter state 7 @12:36:40.47 SPRsummary:12.9RIU,154RIU,376RIU,38.6RIU after 1920s @12:50:57.51 delay 10 seconds @12:51:07.53 SPR.command "start 2 0" @12:51:10.24 Waiting up to 11:00 for SPR to enter state 1 @12:53:07.90 SPR.command "hold_on" @12:53:09.28 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:53:10.33 Lysis2.seek 275uL @12:53:10.43 Lysis1.seek 275uL @12:53:41.57 delay 15 seconds @12:53:56.59 SPR.command "hold_off" @12:53:59.54 Waiting up to 1:00:00 for SPR to enter state 7 @13:24:32.15 delay 10 seconds @13:24:42.17 SPR.command "start 2 0" @13:24:44.85 Waiting up to 11:00 for SPR to enter state 1 @13:26:42.53 SPR.command "hold_on" @13:26:43.90 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:26:44.57 Lysis2.seek 50uL @13:26:44.66 Lysis1.seek 50uL @13:27:15.85 delay 15 seconds @13:27:30.87 SPR.command "hold_off" @13:27:34.15 Waiting up to 1:00:00 for SPR to enter state 7 @13:58:06.71 Additional SPR Analyses Skipped due to Insufficient Volume @13:58:06.95 Intake.close @13:58:07.00 Exhaust.close @13:58:07.14 Bypass.close @13:58:07.23 Handoff.select :close @13:58:07.50
Gate.power :heat,:OFF @13:58:07.53 Gate.power :cartridge,:OFF @13:58:07.69 PinPort.in :unlocking @13:58:07.77 PinPort.seek :clear @13:58:08.27 Carousel.in :default @13:58:08.42 Carousel.home.seek 58 @13:58:08.81 Plunge.seek :locked @13:58:09.01 Archive.in :unlocking @13:58:09.17 Archive.seek :clear @13:58:09.28 Air.in :unlocking @13:58:09.48 Air.seek :clear @13:58:09.64 Lysis1.in :unlocking @13:58:09.79 Lysis1.seek :clear @13:58:09.95 Lysis2.in :unlocking @13:58:10.04 Lysis2.seek :clear @13:58:30.33 Ended log spr24sep1712h.spr.bz2 @13:58:30.35
SPR.command "stats_off" @13:58:31.29 Gate.power :analytic1,:OFF @13:58:31.31 Gate.power :motor,:OFF @13:58:31.35 Gate.power :core,:OFF @13:58:31.38 Gate.power :main,:OFF @13:58:31.42 Safely stopped and ready to power off @13:58:32.12 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:58:32.22 Session Ended @12:23:04.67PDT18-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @12:23:06.38 >BREAK< ESP3 Gateway v1.43 for core bus @12:23:06.41 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:23:09.88 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @12:23:10.55 Sea pressure (Abs) tare is 6% of full scale @12:23:14.16 Polling Can every 2:00 @12:23:14.21 Loading /home/esp/esp2/bin/processAll mission @12:23:14.45 Sea pressure (Abs) tare is 6% of full scale @12:23:14.46 Sampling default goal volumes @12:23:14.48 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @12:23:14.65 Gate.power :sampler,:OFF,limit to 3500mA @12:23:14.66 Gate.power :skimmer,:OFF,limit to 3500mA @12:23:14.67 Gate.power :analytic1,:ON,limit to 4000mA @12:23:15.69 SPR Module 1, FW Rev. B, FPGA Rev. C @12:23:15.80
Plunge.configure :default @12:23:16.01 SamplePump.configure :default @12:23:16.15 Toroid.configure :default @12:23:16.28 Carousel.configure :default @12:23:16.41 Abs.configure :default @12:23:16.55 PhaseSeparator.configure :default @12:23:16.68 Archive.configure :default @12:23:16.81 Air.configure :default @12:23:16.92 Lysis1.configure :default @12:23:17.03 Lysis2.configure :default @12:23:17.16 PinPort.configure :default @12:23:17.21 All elf controllers running firmware version 4.92 @12:23:17.53 Plunge.in :default @12:23:18.02 Plunge.home.jog 0 @12:23:18.26 Lysis2.in :default @12:23:18.31 Lysis1.in :default @12:23:18.35 Archive.in :default @12:23:18.37 Carousel.in :default @12:23:18.39 PinPort.in :default @12:23:18.41 Air.in :default @12:23:18.43 Toroid.in :default @12:23:18.69 Lysis1.home.jog 0 @12:23:18.73 Lysis2.home.jog 0 @12:23:18.75 Archive.home.jog 0 @12:23:18.77 Carousel.home.jog 0 @12:23:18.79 PinPort.home.jog 0 @12:23:18.81 Air.home.jog 0 @12:23:18.83 Toroid.home.jog 0 @12:23:19.89 Lysis1.in :homing @12:23:19.98 Air.in :homing @12:23:20.02 Toroid.in :homing @12:23:20.04 Lysis2.in :homing @12:23:20.06 Plunge.in :homing @12:23:20.08 Archive.in :homing @12:23:20.16 PinPort.in :homing @12:23:20.33 Lysis1.jog 1392 @12:23:20.41 Air.jog 1376 @12:23:20.45 Toroid.jog 632 @12:23:20.87 Lysis2.jog 1406 @12:23:20.90 Archive.jog 1397 @12:23:20.93 Plunge.jog 163 @12:23:20.99 PinPort.jog -110 @12:23:21.55 PinPort.setRawPosition! 0 @12:23:21.60 PinPort.seek :home @12:23:21.71 PinPort.in :default @12:23:21.79 Plunge.setRawPosition! 50 @12:23:21.85 Plunge.seek :home @12:23:22.13 Lysis1.setRawPosition! 50 @12:23:22.16 Plunge.in :default @12:23:22.28 Lysis1.seek :home @12:23:22.38 Lysis2.setRawPosition! 50 @12:23:22.44 Air.setRawPosition! 50 @12:23:22.49 Lysis2.seek :home @12:23:22.61 Air.seek :home @12:23:22.73 Archive.setRawPosition! 50 @12:23:22.75 Lysis1.in :default @12:23:22.89 Archive.seek :home @12:23:23.01 Air.in :default @12:23:23.03 Lysis2.in :default @12:23:23.17 Archive.in :default @12:23:23.26 Toroid.setRawPosition! 0 @12:23:23.31 Toroid.seek :home @12:23:23.40 Toroid.in :default @12:23:23.55 Toroid.seek :clear @12:23:25.27
Carousel.in :default @12:23:25.41 Carousel.home.seek 58 @12:23:25.84 Selecting Cartridge 58 @12:23:26.12 Carousel.seek 58 @12:23:26.37 Lysis1.in :fast @12:23:26.42 Lysis1.seek :locked @12:23:29.03 Plunge.seek :locked @12:23:29.19 Air.in :fast @12:23:29.24 Air.seek :locked @12:23:30.21
Carousel.reconfigure :locked @12:23:30.36 Powering cartridge bus @12:23:33.03 Gate.setIR 1750,5000,0,6 @12:23:33.15 Gate.setIR 0,5000,0,6 @12:23:33.21 PuckHeater.configure :default @12:23:33.27 Gate.power :heat,:ON,limit to 5000mA @12:23:33.94 Mounted lysis Cartridge @12:23:33.97 WARNING: Using default puck temperature sensor calibration @12:23:33.98 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:23:35.51 SPR.command "read_sequences" @12:23:35.57 SPR.command "stats_on" @12:23:36.90 SPR.command "start 0 1" @12:23:37.15 SPR.start /var/log/esp/spr24sep1812h.spr.bz2 @12:23:39.19 Waiting for baseline SPR stabilization @12:23:39.21 Handoff.select :waste @12:23:39.36 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:23:45.98 Syringe tare is 51% of full scale at 22C (drifted 0%) @12:23:46.00 PinPort.in :locking @12:23:46.05 PinPort.seek :locked @12:23:46.55 Archive.in :fast @12:23:46.64 Lysis2.in :fast @12:23:46.69 Lysis1.seek 1100uL @12:23:46.72 Archive.seek :full @12:23:46.84 Lysis2.seek 1100uL @12:23:51.16 delayUntil "12:33:38.77" @12:24:17.86 Session Begun @12:33:38.79 Lysis2.in :DAmix @12:33:38.93 Lysis1.in :DAmix @12:33:39.52 Lysis2.seek 950uL @12:33:39.63 Lysis1.seek 950uL @12:34:00.49 delay 5 seconds @12:34:05.51 Handoff.select SPR @12:34:05.67 SPR.command "start 2 14" @12:34:06.28 SPR stabilized @12:34:06.47 delay 10 seconds @12:34:16.49 SPR.command "start 2 0" @12:34:19.65 Waiting up to 11:00 for SPR to enter state 1 @12:36:17.30 SPR.command "hold_on" @12:36:18.71 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @12:36:19.36 Lysis2.seek 500uL @12:36:19.46 Lysis1.seek 500uL @12:37:21.68 delay 15 seconds @12:37:36.71 SPR.command "hold_off" @12:37:40.17 Waiting up to 1:00:00 for SPR to enter state 7 @12:49:56.42 Session Begun @12:50:04.41 -> showlog @12:53:55.73 SPRsummary:9.88RIU,127RIU,295RIU,21.3RIU after 1920s @13:08:12.76 delay 10 seconds @13:08:22.78 SPR.command "start 2 0" @13:08:25.50 Waiting up to 11:00 for SPR to enter state 1 @13:10:23.15 SPR.command "hold_on" @13:10:24.55 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:10:25.24 Lysis2.seek 275uL @13:10:25.30 Lysis1.seek 275uL @13:10:56.60 delay 15 seconds @13:11:11.62 SPR.command "hold_off" @13:11:14.79 Waiting up to 1:00:00 for SPR to enter state 7 @13:41:47.39 delay 10 seconds @13:41:57.43 SPR.command "start 2 0" @13:42:00.63 Waiting up to 11:00 for SPR to enter state 1 @13:43:58.72 SPR.command "hold_on" @13:44:00.11 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:44:00.79 Lysis2.seek 50uL @13:44:00.86 Lysis1.seek 50uL @13:44:32.09 delay 15 seconds @13:44:47.12 SPR.command "hold_off" @13:44:50.35 Waiting up to 1:00:00 for SPR to enter state 7 @14:15:22.89 Additional SPR Analyses Skipped due to Insufficient Volume @14:15:23.15 Intake.close @14:15:23.26 Exhaust.close @14:15:23.35 Bypass.close @14:15:23.51 Handoff.select :close @14:15:23.86
Gate.power :heat,:OFF @14:15:23.88 Gate.power :cartridge,:OFF @14:15:24.03 PinPort.in :unlocking @14:15:24.11 PinPort.seek :clear @14:15:24.66 Carousel.in :default @14:15:24.87 Carousel.home.seek 58 @14:15:25.25 Plunge.seek :locked @14:15:25.52 Archive.in :unlocking @14:15:25.70 Archive.seek :clear @14:15:25.84 Air.in :unlocking @14:15:26.00 Air.seek :clear @14:15:26.15 Lysis1.in :unlocking @14:15:26.33 Lysis1.seek :clear @14:15:26.41 Lysis2.in :unlocking @14:15:26.62 Lysis2.seek :clear @14:15:46.99 Ended log spr24sep1812h.spr.bz2 @14:15:47.02
SPR.command "stats_off" @14:15:48.03 Gate.power :analytic1,:OFF @14:15:48.06 Gate.power :motor,:OFF @14:15:48.11 Gate.power :core,:OFF @14:15:48.17 Gate.power :main,:OFF @14:15:48.20 Safely stopped and ready to power off @14:15:48.98 Cartridge::None in MAIN -- Not enough standard cartridges remain @14:15:49.09 Session Ended @14:15:49.15 Session Ended @15:41:04.03PDT18-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @15:41:05.76 >BREAK< ESP3 Gateway v1.43 for core bus @15:41:05.79 >BREAK< ESP3 Gateway v1.43 for cartridge bus @15:41:09.47 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @15:41:10.15 Sea pressure (Abs) tare is 6% of full scale @15:41:13.89 Polling Can every 2:00 @15:41:13.94 Loading /home/esp/esp2/bin/processAll mission @15:41:14.18 Sea pressure (Abs) tare is 6% of full scale @15:41:14.20 Sampling default goal volumes @15:41:14.22 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @15:41:14.38 Gate.power :sampler,:OFF,limit to 3500mA @15:41:14.39 Gate.power :skimmer,:OFF,limit to 3500mA @15:41:14.44 Gate.power :analytic1,:ON,limit to 4000mA @15:41:15.03 SPR Module 1, FW Rev. B, FPGA Rev. C @15:41:15.21
Plunge.configure :default @15:41:15.34 SamplePump.configure :default @15:41:15.52 Toroid.configure :default @15:41:15.57 Toroid.hold @15:41:15.64 Toroid.configure :default @15:41:15.77 Carousel.configure :default @15:41:15.82 Carousel.hold @15:41:15.89 Carousel.configure :default @15:41:16.03 Abs.configure :default @15:41:16.16 PhaseSeparator.configure :default @15:41:16.30 Archive.configure :default @15:41:16.43 Air.configure :default @15:41:16.55 Lysis1.configure :default @15:41:16.66 Lysis2.configure :default @15:41:16.80 PinPort.configure :default @15:41:16.85 All elf controllers running firmware version 4.92 @15:41:17.66 Toroid.seek :clear @15:41:17.83
Carousel.in :default @15:41:17.94 Carousel.home.seek 58 @15:41:18.21 Selecting Cartridge 58 @15:41:18.51 Carousel.seek 58 @15:41:18.75 Lysis1.in :fast @15:41:18.81 Lysis1.seek :locked @15:41:21.43 Plunge.seek :locked @15:41:22.07 Air.in :fast @15:41:22.13 Air.seek :locked @15:41:23.10
Carousel.reconfigure :locked @15:41:23.25 Powering cartridge bus @15:41:25.92 Gate.setIR 1750,5000,0,6 @15:41:26.04 Gate.setIR 0,5000,0,6 @15:41:26.11 PuckHeater.configure :default @15:41:26.17 Gate.power :heat,:ON,limit to 5000mA @15:41:26.85 Mounted lysis Cartridge @15:41:26.88 WARNING: Using default puck temperature sensor calibration @15:41:26.89 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:41:28.44 SPR.command "read_sequences" @15:41:29.13 SPR.command "stats_on" @15:41:29.29 SPR.command "start 0 1" @15:41:30.00 SPR.start /var/log/esp/spr24sep1815h.spr.bz2 @15:41:31.99 Waiting for baseline SPR stabilization @15:41:32.01 Handoff.select :waste @15:41:32.14 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:41:38.77 Syringe tare is 51% of full scale at 22C (drifted 0%) @15:41:38.79 PinPort.in :locking @15:41:38.84 PinPort.seek :locked @15:41:39.34 Archive.in :fast @15:41:39.48 Lysis2.in :fast @15:41:39.53 Archive.seek :full @15:41:39.58 Lysis1.seek 1100uL @15:41:39.72 Lysis2.seek 1100uL @15:41:44.01 delayUntil "15:51:31.52" @15:51:31.54 Lysis2.in :DAmix @15:51:31.65 Lysis1.in :DAmix @15:51:32.21 Lysis2.seek 950uL @15:51:32.30 Lysis1.seek 950uL @15:51:53.10 delay 5 seconds @15:51:58.12 Handoff.select SPR @15:51:58.25 SPR.command "start 2 14" @15:51:59.07 SPR stabilized @15:51:59.21 delay 10 seconds @15:52:09.24 SPR.command "start 2 0" @15:52:12.44 Waiting up to 11:00 for SPR to enter state 1 @15:54:10.09 SPR.command "hold_on" @15:54:11.50 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:54:12.09 Lysis2.seek 500uL @15:54:12.15 Lysis1.seek 500uL @15:55:14.31 delay 15 seconds @15:55:29.34 SPR.command "hold_off" @15:55:32.50 Waiting up to 1:00:00 for SPR to enter state 7 @15:56:16.31 Session Begun @15:56:21.53 -> showlog @16:11:48.51 SPRsummary:-2.8RIU,128RIU,270RIU,-1.01RIU after 1920s @16:26:05.58 delay 10 seconds @16:26:15.61 SPR.command "start 2 0" @16:26:18.28 Waiting up to 11:00 for SPR to enter state 1 @16:28:15.93 SPR.command "hold_on" @16:28:17.33 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:28:18.06 Lysis2.seek 275uL @16:28:18.12 Lysis1.seek 275uL @16:28:49.33 delay 15 seconds @16:29:04.35 SPR.command "hold_off" @16:29:07.58 Waiting up to 1:00:00 for SPR to enter state 7 @16:59:40.20 delay 10 seconds @16:59:50.22 SPR.command "start 2 0" @16:59:52.90 Waiting up to 11:00 for SPR to enter state 1 @17:01:50.56 SPR.command "hold_on" @17:01:51.96 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @17:01:52.60 Lysis2.seek 50uL @17:01:52.68 Lysis1.seek 50uL @17:02:23.84 delay 15 seconds @17:02:38.86 SPR.command "hold_off" @17:02:42.72 Waiting up to 1:00:00 for SPR to enter state 7 @17:33:15.69 Additional SPR Analyses Skipped due to Insufficient Volume @17:33:15.94 Intake.close @17:33:15.99 Exhaust.close @17:33:16.06 Bypass.close @17:33:16.19 Handoff.select :close @17:33:16.50
Gate.power :heat,:OFF @17:33:16.55 Gate.power :cartridge,:OFF @17:33:16.69 PinPort.in :unlocking @17:33:16.77 PinPort.seek :clear @17:33:17.27 Carousel.in :default @17:33:17.47 Carousel.home.seek 58 @17:33:17.86 Plunge.seek :locked @17:33:18.06 Archive.in :unlocking @17:33:18.22 Archive.seek :clear @17:33:18.29 Air.in :unlocking @17:33:18.52 Air.seek :clear @17:33:18.68 Lysis1.in :unlocking @17:33:18.83 Lysis1.seek :clear @17:33:18.97 Lysis2.in :unlocking @17:33:19.06 Lysis2.seek :clear @17:33:39.59 Ended log spr24sep1815h.spr.bz2 @17:33:39.61
SPR.command "stats_off" @17:33:39.88 Gate.power :analytic1,:OFF @17:33:39.90 Gate.power :motor,:OFF @17:33:39.94 Gate.power :core,:OFF @17:33:39.98 Gate.power :main,:OFF @17:33:40.02 Safely stopped and ready to power off @17:33:40.71 Cartridge::None in MAIN -- Not enough standard cartridges remain @17:33:40.80 Session Ended nohup: procelAll: No such file or directory @10:06:46.78PDT19-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @10:06:48.54 >BREAK< ESP3 Gateway v1.43 for core bus @10:06:48.56 >BREAK< ESP3 Gateway v1.43 for cartridge bus @10:06:52.13 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @10:06:52.80 Sea pressure (Abs) tare is 6% of full scale @10:06:56.42 Polling Can every 2:00 @10:06:56.47 Loading /home/esp/esp2/bin/processAll mission @10:06:56.71 Sea pressure (Abs) tare is 6% of full scale @10:06:56.72 Sampling default goal volumes @10:06:56.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @10:06:56.91 Gate.power :sampler,:OFF,limit to 3500mA @10:06:56.92 Gate.power :skimmer,:OFF,limit to 3500mA @10:06:56.97 Gate.power :analytic1,:ON,limit to 4000mA @10:06:57.69 Plunge.configure :default @10:06:57.83 SamplePump.configure :default @10:06:57.93 SPR Module 1, FW Rev. B, FPGA Rev. C @10:06:58.03
Toroid.configure :default @10:06:58.17 Carousel.configure :default @10:06:58.31 Abs.configure :default @10:06:58.45 PhaseSeparator.configure :default @10:06:58.59 Archive.configure :default @10:06:58.84 Air.configure :default @10:06:58.96 Lysis1.configure :default @10:06:59.08 Lysis2.configure :default @10:06:59.22 PinPort.configure :default @10:06:59.27 All elf controllers running firmware version 4.92 @10:06:59.54 Plunge.in :default @10:07:00.05 Plunge.home.jog 0 @10:07:00.27 Lysis2.in :default @10:07:00.32 Lysis1.in :default @10:07:00.36 Air.in :default @10:07:00.38 Archive.in :default @10:07:00.40 Carousel.in :default @10:07:00.43 Toroid.in :default @10:07:00.44 PinPort.in :default @10:07:00.73 Lysis1.home.jog 0 @10:07:00.82 Lysis2.home.jog 0 @10:07:00.85 Air.home.jog 0 @10:07:00.87 Archive.home.jog 0 @10:07:00.89 PinPort.home.jog 0 @10:07:00.91 Carousel.home.jog 0 @10:07:00.95 Toroid.home.jog 0 @10:07:02.00 Lysis2.in :homing @10:07:02.11 Lysis1.in :homing @10:07:02.14 Toroid.in :homing @10:07:02.15 Archive.in :homing @10:07:02.19 PinPort.in :homing @10:07:02.22 Air.in :homing @10:07:02.26 Plunge.in :homing @10:07:02.47 Lysis2.jog 1409 @10:07:02.55 Lysis1.jog 1396 @10:07:02.99 Archive.jog 1401 @10:07:03.04 PinPort.jog -109 @10:07:03.07 Toroid.jog 632 @10:07:03.11 Air.jog 1375 @10:07:03.30 Plunge.jog 159 @10:07:03.69 PinPort.setRawPosition! 0 @10:07:03.74 PinPort.seek :home @10:07:03.84 PinPort.in :default @10:07:04.00 Plunge.setRawPosition! 50 @10:07:04.06 Plunge.seek :home @10:07:04.31 Lysis2.setRawPosition! 50 @10:07:04.41 Lysis1.setRawPosition! 50 @10:07:04.45 Archive.setRawPosition! 50 @10:07:04.47 Lysis2.seek :home @10:07:04.49 Plunge.in :default @10:07:04.68 Lysis1.seek :home @10:07:04.70 Archive.seek :home @10:07:04.97 Air.setRawPosition! 50 @10:07:04.99 Lysis2.in :default @10:07:05.11 Air.seek :home @10:07:05.22 Lysis1.in :default @10:07:05.24 Archive.in :default @10:07:05.39 Air.in :default @10:07:05.53 Toroid.setRawPosition! 0 @10:07:05.60 Toroid.seek :home @10:07:05.69 Toroid.in :default @10:07:05.78 Toroid.seek :clear @10:07:07.49
Carousel.in :default @10:07:07.62 Carousel.home.seek 58 @10:07:07.89 Selecting Cartridge 58 @10:07:08.19 Carousel.seek 58 @10:07:08.45 Lysis1.in :fast @10:07:08.51 Lysis1.seek :locked @10:07:11.25 Plunge.seek :locked @10:07:11.59 Air.in :fast @10:07:11.69 Air.seek :locked @10:07:11.95 Session Begun @10:07:12.71
Carousel.reconfigure :locked @10:07:12.89 Powering cartridge bus @10:07:15.56 Gate.setIR 1750,5000,0,6 @10:07:15.69 Gate.setIR 0,5000,0,6 @10:07:15.78 PuckHeater.configure :default @10:07:15.87 Gate.power :heat,:ON,limit to 5000mA @10:07:16.54 Mounted lysis Cartridge @10:07:16.59 WARNING: Using default puck temperature sensor calibration @10:07:16.61 Using default cartridge heat loss curve for HeatingAccelInCarousel @10:07:18.20 SPR.command "read_sequences" @10:07:18.64 SPR.command "stats_on" @10:07:19.38 SPR.command "start 0 1" @10:07:20.09 SPR.start /var/log/esp/spr24sep1910h.spr.bz2 @10:07:21.99 Waiting for baseline SPR stabilization @10:07:22.01 Handoff.select :waste @10:07:22.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @10:07:28.84 Syringe tare is 51% of full scale at 22C (drifted -0%) @10:07:28.90 PinPort.in :locking @10:07:29.00 PinPort.seek :locked @10:07:29.53 Archive.in :fast @10:07:29.70 Lysis2.in :fast @10:07:29.83 Lysis1.seek 1100uL @10:07:29.87 Archive.seek :full @10:07:30.07 Lysis2.seek 1100uL @10:07:34.35 delayUntil "10:17:21.95" @10:17:21.97 Lysis2.in :DAmix @10:17:22.11 Lysis1.in :DAmix @10:17:22.67 Lysis2.seek 950uL @10:17:22.72 Lysis1.seek 950uL @10:17:43.63 delay 5 seconds @10:17:48.65 Handoff.select SPR @10:17:48.81 SPR.command "start 2 14" @10:17:49.53 SPR stabilized @10:17:49.71 delay 10 seconds @10:17:59.73 SPR.command "start 2 0" @10:18:02.90 Waiting up to 11:00 for SPR to enter state 1 @10:20:00.54 SPR.command "hold_on" @10:20:01.95 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @10:20:02.60 Lysis2.seek 500uL @10:20:02.65 Lysis1.seek 500uL @10:21:04.84 delay 15 seconds @10:21:19.86 SPR.command "hold_off" @10:21:22.46 Waiting up to 1:00:00 for SPR to enter state 7 @10:37:38.01 SPRsummary:6.18RIU,138RIU,288RIU,20.9RIU after 1920s @10:51:55.06 delay 10 seconds @10:52:05.09 SPR.command "start 2 0" @10:52:07.79 Waiting up to 11:00 for SPR to enter state 1 @10:54:05.46 SPR.command "hold_on" @10:54:06.84 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @10:54:07.56 Lysis2.seek 275uL @10:54:07.60 Lysis1.seek 275uL @10:54:38.80 delay 15 seconds @10:54:53.82 SPR.command "hold_off" @10:54:57.09 Waiting up to 1:00:00 for SPR to enter state 7 @11:25:29.69 delay 10 seconds @11:25:39.71 SPR.command "start 2 0" @11:25:42.41 Waiting up to 11:00 for SPR to enter state 1 @11:27:40.07 SPR.command "hold_on" @11:27:41.47 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @11:27:42.12 Lysis2.seek 50uL @11:27:42.21 Lysis1.seek 50uL @11:28:13.37 delay 15 seconds @11:28:28.39 SPR.command "hold_off" @11:28:31.71 Waiting up to 1:00:00 for SPR to enter state 7 @11:59:04.25 Additional SPR Analyses Skipped due to Insufficient Volume @11:59:04.47 Intake.close @11:59:04.57 Exhaust.close @11:59:04.67 Bypass.close @11:59:04.73 Handoff.select :close @11:59:05.03
Gate.power :heat,:OFF @11:59:05.07 Gate.power :cartridge,:OFF @11:59:05.22 PinPort.in :unlocking @11:59:05.29 PinPort.seek :clear @11:59:05.81 Carousel.in :default @11:59:05.96 Carousel.home.seek 58 @11:59:06.35 Plunge.seek :locked @11:59:06.57 Archive.in :unlocking @11:59:06.74 Archive.seek :clear @11:59:06.90 Air.in :unlocking @11:59:07.00 Air.seek :clear @11:59:07.15 Lysis1.in :unlocking @11:59:07.31 Lysis1.seek :clear @11:59:07.50 Lysis2.in :unlocking @11:59:07.59 Lysis2.seek :clear @11:59:28.48 Ended log spr24sep1910h.spr.bz2 @11:59:28.51
SPR.command "stats_off" @11:59:29.34 Gate.power :analytic1,:OFF @11:59:29.36 Gate.power :motor,:OFF @11:59:29.40 Gate.power :core,:OFF @11:59:29.47 Gate.power :main,:OFF @11:59:29.51 Safely stopped and ready to power off @11:59:30.22 Cartridge::None in MAIN -- Not enough standard cartridges remain @11:59:30.31 Session Ended @13:17:34.44PDT19-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @13:17:36.15 >BREAK< ESP3 Gateway v1.43 for core bus @13:17:36.18 >BREAK< ESP3 Gateway v1.43 for cartridge bus @13:17:39.78 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @13:17:40.49 Sea pressure (Abs) tare is 6% of full scale @13:17:44.26 Polling Can every 2:00 @13:17:44.30 Loading /home/esp/esp2/bin/processAll mission @13:17:44.53 Sea pressure (Abs) tare is 6% of full scale @13:17:44.54 Sampling default goal volumes @13:17:44.56 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:17:44.61 Gate.power :sampler,:OFF,limit to 3500mA @13:17:44.62 Gate.power :skimmer,:OFF,limit to 3500mA @13:17:44.64 Gate.power :main,:ON,limit to 5000mA @13:17:44.65 Gate.power :analytic1,:ON,limit to 4000mA @13:17:44.84 Powering core bus @13:17:47.08 SPR Module 1, FW Rev. B, FPGA Rev. C @13:17:47.62
Plunge.configure :default @13:17:47.69 SamplePump.configure :default @13:17:47.77 Toroid.configure :default @13:17:47.85 Carousel.configure :default @13:17:47.93 Abs.configure :default @13:17:48.00 PhaseSeparator.configure :default @13:17:48.08 Archive.configure :default @13:17:48.15 Air.configure :default @13:17:48.21 Lysis1.configure :default @13:17:48.26 Lysis2.configure :default @13:17:48.34 PinPort.configure :default @13:17:48.39 All elf controllers running firmware version 4.92 @13:17:48.41 Gate.power :motor,:ON,limit to 5000mA @13:17:48.58 Plunge.home.jog 0 @13:17:49.32 PinPort.home.jog 0 @13:17:49.42 Lysis2.home.jog 0 @13:17:49.45 Air.home.jog 0 @13:17:49.53 Carousel.home.jog 0 @13:17:49.60 Lysis1.home.jog 0 @13:17:49.73 Toroid.home.jog 0 @13:17:49.77 Archive.home.jog 0 @13:17:50.95 Plunge.in :homing @13:17:51.27 Plunge.jog 1607 @13:17:51.34 Lysis2.in :homing @13:17:51.38 Air.in :homing @13:17:51.40 Toroid.in :homing @13:17:51.46 PinPort.in :homing @13:17:51.48 Lysis1.in :homing @13:17:51.50 Archive.in :homing @13:17:51.76 Lysis2.jog 1406 @13:17:51.86 Air.jog 1381 @13:17:51.93 PinPort.jog -110 @13:17:51.99 Archive.jog 1399 @13:17:52.03 Toroid.jog 627 @13:17:52.07 Lysis1.jog 1400 @13:17:52.41 PinPort.setRawPosition! 0 @13:17:52.46 PinPort.seek :home @13:17:52.55 PinPort.in :default @13:17:52.83 Plunge.setRawPosition! 50 @13:17:52.94 Plunge.seek :home @13:17:53.80 Lysis1.setRawPosition! 50 @13:17:53.93 Air.setRawPosition! 50 @13:17:53.95 Archive.setRawPosition! 50 @13:17:53.97 Lysis2.setRawPosition! 50 @13:17:54.16 Lysis1.seek :home @13:17:54.21 Air.seek :home @13:17:54.25 Archive.seek :home @13:17:54.34 Lysis2.seek :home @13:17:54.68 Lysis1.in :default @13:17:54.73 Toroid.setRawPosition! 0 @13:17:54.77 Plunge.in :default @13:17:54.89 Toroid.seek :home @13:17:55.08 Air.in :default @13:17:55.13 Archive.in :default @13:17:55.15 Lysis2.in :default @13:17:55.17 Toroid.in :default @13:17:55.64 Toroid.seek :clear @13:17:57.37
Carousel.in :default @13:17:57.52 Carousel.home.seek 58 @13:17:57.80 Selecting Cartridge 57 @13:17:58.10 Carousel.seek 57 @13:17:58.75 Lysis1.in :fast @13:17:58.86 Lysis1.seek :locked @13:17:59.49 Session Begun @13:18:01.58 Plunge.seek :locked @13:18:01.80 Air.in :fast @13:18:01.87 Air.seek :locked @13:18:02.87
Carousel.reconfigure :locked @13:18:03.06 Powering cartridge bus @13:18:05.72 Gate.setIR 1750,5000,0,6 @13:18:05.86 Gate.setIR 0,5000,0,6 @13:18:05.95 PuckHeater.configure :default @13:18:06.04 Gate.power :heat,:ON,limit to 5000mA @13:18:06.73 Mounted lysis Cartridge @13:18:06.76 WARNING: Using default puck temperature sensor calibration @13:18:06.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:18:08.37 SPR.command "read_sequences" @13:18:08.73 SPR.command "stats_on" @13:18:08.90 SPR.command "start 0 1" @13:18:09.61 SPR.start /var/log/esp/spr24sep1913h.spr.bz2 @13:18:11.59 Waiting for baseline SPR stabilization @13:18:11.61 Handoff.select :waste @13:18:11.84 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:18:18.47 Syringe tare is 51% of full scale at 22C (drifted 0%) @13:18:18.50 PinPort.in :locking @13:18:18.61 PinPort.seek :locked @13:18:19.17 Archive.in :fast @13:18:19.27 Lysis1.seek 1100uL @13:18:19.34 Lysis2.in :fast @13:18:19.50 Archive.seek :full @13:18:19.70 Lysis2.seek 1100uL @13:18:24.01 delayUntil "13:28:11.12" @13:28:11.13 Lysis2.in :DAmix @13:28:11.28 Lysis1.in :DAmix @13:28:11.84 Lysis2.seek 950uL @13:28:11.90 Lysis1.seek 950uL @13:28:32.80 delay 5 seconds @13:28:37.82 Handoff.select SPR @13:28:37.99 SPR.command "start 2 14" @13:28:39.16 SPR stabilized @13:28:39.35 delay 10 seconds @13:28:49.37 SPR.command "start 2 0" @13:28:52.53 Waiting up to 11:00 for SPR to enter state 1 @13:30:50.62 SPR.command "hold_on" @13:30:52.04 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @13:30:52.67 Lysis2.seek 500uL @13:30:52.73 Lysis1.seek 500uL @13:31:54.95 delay 15 seconds @13:32:10.00 SPR.command "hold_off" @13:32:13.50 Waiting up to 1:00:00 for SPR to enter state 7 @13:48:29.05 SPRsummary:-2.89RIU,132RIU,270RIU,5.65RIU after 1921s @14:02:46.11 delay 10 seconds @14:02:56.13 SPR.command "start 2 0" @14:02:58.82 Waiting up to 11:00 for SPR to enter state 1 @14:04:56.47 SPR.command "hold_on" @14:04:57.87 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:04:58.95 Lysis2.seek 275uL @14:04:58.99 Lysis1.seek 275uL @14:05:30.16 delay 15 seconds @14:05:45.18 SPR.command "hold_off" @14:05:48.63 Waiting up to 1:00:00 for SPR to enter state 7 @14:36:21.67 delay 10 seconds @14:36:31.70 SPR.command "start 2 0" @14:36:34.91 Waiting up to 11:00 for SPR to enter state 1 @14:38:33.01 SPR.command "hold_on" @14:38:34.39 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:38:35.09 Lysis2.seek 50uL @14:38:35.18 Lysis1.seek 50uL @14:39:06.38 delay 15 seconds @14:39:21.41 SPR.command "hold_off" @14:39:24.64 Waiting up to 1:00:00 for SPR to enter state 7 @15:09:57.19 Additional SPR Analyses Skipped due to Insufficient Volume @15:09:57.47 Handoff.select :close @15:09:57.66
Gate.power :heat,:OFF @15:09:57.68 Gate.power :cartridge,:OFF @15:09:57.81 PinPort.in :unlocking @15:09:57.88 PinPort.seek :clear @15:09:58.39 Carousel.in :default @15:09:58.54 Carousel.home.seek 57 @15:09:58.89 Plunge.seek :locked @15:09:59.14 Archive.in :unlocking @15:09:59.28 Archive.seek :clear @15:09:59.45 Air.in :unlocking @15:09:59.62 Air.seek :clear @15:09:59.75 Lysis1.in :unlocking @15:09:59.90 Lysis1.seek :clear @15:10:00.06 Lysis2.in :unlocking @15:10:00.17 Lysis2.seek :clear @15:10:21.09 Ended log spr24sep1913h.spr.bz2 @15:10:21.11
SPR.command "stats_off" @15:10:21.42 Gate.power :analytic1,:OFF @15:10:21.46 Gate.power :motor,:OFF @15:10:21.49 Gate.power :core,:OFF @15:10:21.53 Gate.power :main,:OFF @15:10:21.56 Safely stopped and ready to power off @15:10:22.34 Gate.power :sampler,:OFF,limit to 3500mA @15:10:22.36 Gate.power :skimmer,:OFF,limit to 3500mA @15:10:25.34 Gate.power :main,:ON,limit to 5000mA @15:10:25.36 Gate.power :analytic1,:ON,limit to 4000mA @15:10:25.52 Powering core bus @15:10:27.88 SPR Module 1, FW Rev. B, FPGA Rev. C @15:10:28.33
Plunge.configure :default @15:10:28.44 SamplePump.configure :default @15:10:28.56 Toroid.configure :default @15:10:28.67 Carousel.configure :default @15:10:28.78 Abs.configure :default @15:10:28.89 PhaseSeparator.configure :default @15:10:28.99 Archive.configure :default @15:10:29.10 Air.configure :default @15:10:29.18 Lysis1.configure :default @15:10:29.26 Lysis2.configure :default @15:10:29.37 PinPort.configure :default @15:10:29.49 All elf controllers running firmware version 4.92 @15:10:29.51 Gate.power :motor,:ON,limit to 5000mA @15:10:29.86 Plunge.home.jog 0 @15:10:30.54 Lysis2.home.jog 0 @15:10:30.61 Lysis1.home.jog 0 @15:10:30.64 Archive.home.jog 0 @15:10:30.68 Toroid.home.jog 0 @15:10:30.79 Carousel.home.jog 0 @15:10:30.86 PinPort.home.jog 0 @15:10:30.90 Air.home.jog 0 @15:10:31.85 Plunge.in :homing @15:10:32.19 Plunge.jog 1602 @15:10:32.28 Lysis2.in :homing @15:10:32.32 Air.in :homing @15:10:32.38 Toroid.in :homing @15:10:32.42 PinPort.in :homing @15:10:32.47 Archive.in :homing @15:10:32.51 Lysis1.in :homing @15:10:32.80 Lysis2.jog 1404 @15:10:32.96 Lysis1.jog 1397 @15:10:33.03 Archive.jog 1400 @15:10:33.09 PinPort.jog -111 @15:10:33.14 Toroid.jog 624 @15:10:33.19 Air.jog 1377 @15:10:33.73 PinPort.setRawPosition! 0 @15:10:33.82 PinPort.seek :home @15:10:33.95 Plunge.setRawPosition! 50 @15:10:34.03 Plunge.seek :home @15:10:34.15 PinPort.in :default @15:10:34.32 Plunge.in :default @15:10:34.46 Lysis2.setRawPosition! 50 @15:10:34.56 Lysis1.setRawPosition! 50 @15:10:34.60 Lysis2.seek :home @15:10:34.79 Lysis1.seek :home @15:10:34.93 Air.setRawPosition! 50 @15:10:34.96 Archive.setRawPosition! 50 @15:10:35.55 Air.seek :home @15:10:35.62 Archive.seek :home @15:10:35.97 Lysis2.in :default @15:10:35.99 Lysis1.in :default @15:10:36.04 Toroid.setRawPosition! 0 @15:10:36.23 Toroid.seek :home @15:10:36.38 Archive.in :default @15:10:36.47 Air.in :default @15:10:36.67 Toroid.in :default @15:10:37.22 Toroid.seek :clear @15:10:39.34
Carousel.in :default @15:10:39.52 Carousel.home.seek 57 @15:10:39.80 Selecting Cartridge 56 @15:10:40.12 Carousel.seek 56 @15:10:40.68 Lysis1.in :fast @15:10:40.76 Lysis1.seek :locked @15:10:43.44 Plunge.seek :locked @15:10:43.64 Air.in :fast @15:10:43.72 Air.seek :locked @15:10:44.72
Carousel.reconfigure :locked @15:10:44.90 Powering cartridge bus @15:10:47.57 Gate.setIR 1750,5000,0,6 @15:10:47.72 Gate.setIR 0,5000,0,6 @15:10:47.81 PuckHeater.configure :default @15:10:47.91 Gate.power :heat,:ON,limit to 5000mA @15:10:48.60 Mounted lysis Cartridge board#Q12558 @15:10:48.66 WARNING: Using default puck temperature sensor calibration @15:10:48.68 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:10:50.29 SPR.command "read_sequences" @15:10:50.42 SPR.command "stats_on" @15:10:50.59 SPR.command "start 0 1" @15:10:51.30 SPR.start /var/log/esp/spr24sep1915h.spr.bz2 @15:10:53.27 Waiting for baseline SPR stabilization @15:10:53.30 Handoff.select :waste @15:10:53.53 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:11:00.17 Syringe tare is 51% of full scale at 22C (drifted 0%) @15:11:00.20 PinPort.in :locking @15:11:00.30 PinPort.seek :locked @15:11:00.85 Archive.in :fast @15:11:00.96 Lysis2.in :fast @15:11:01.03 Lysis1.seek 1100uL @15:11:01.09 Archive.seek :full @15:11:01.25 Lysis2.seek 1100uL @15:11:05.59 delayUntil "15:20:52.83" @15:20:52.85 Lysis2.in :DAmix @15:20:52.99 Lysis1.in :DAmix @15:20:53.57 Lysis2.seek 950uL @15:20:53.60 Lysis1.seek 950uL @15:21:14.49 delay 5 seconds @15:21:19.52 Handoff.select SPR @15:21:19.68 SPR.command "start 2 14" @15:21:20.36 SPR stabilized @15:21:20.54 delay 10 seconds @15:21:30.56 SPR.command "start 2 0" @15:21:34.24 Waiting up to 11:00 for SPR to enter state 1 @15:23:32.34 SPR.command "hold_on" @15:23:33.73 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:23:34.39 Lysis2.seek 500uL @15:23:34.44 Lysis1.seek 500uL @15:24:36.69 delay 15 seconds @15:24:51.73 SPR.command "hold_off" @15:24:55.19 Waiting up to 1:00:00 for SPR to enter state 7 @15:41:10.76 SPRsummary:-3.19RIU,121RIU,273RIU,1.65RIU after 1921s @15:55:27.79 delay 10 seconds @15:55:37.82 SPR.command "start 2 0" @15:55:40.52 Waiting up to 11:00 for SPR to enter state 1 @15:57:38.18 SPR.command "hold_on" @15:57:40.08 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @15:57:40.77 Lysis2.seek 275uL @15:57:40.82 Lysis1.seek 275uL @15:58:12.04 delay 15 seconds @15:58:27.06 SPR.command "hold_off" @15:58:29.82 Waiting up to 1:00:00 for SPR to enter state 7 @16:29:01.50 delay 10 seconds @16:29:11.53 SPR.command "start 2 0" @16:29:14.19 Waiting up to 11:00 for SPR to enter state 1 @16:31:11.85 SPR.command "hold_on" @16:31:13.24 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:31:13.97 Lysis2.seek 50uL @16:31:14.01 Lysis1.seek 50uL @16:31:45.21 delay 15 seconds @16:32:00.23 SPR.command "hold_off" @16:32:04.00 Waiting up to 1:00:00 for SPR to enter state 7 @16:35:45.23 Session Begun @16:35:49.76 -> showlog -100 @16:49:49.69 Session Ended @16:58:37.95 Session Ended @17:02:36.98 Additional SPR Analyses Skipped due to Insufficient Volume @17:02:37.19 Handoff.select :close @17:02:37.35
Gate.power :heat,:OFF @17:02:37.37 Gate.power :cartridge,:OFF @17:02:37.48 PinPort.in :unlocking @17:02:37.53 PinPort.seek :clear @17:02:38.01 Carousel.in :default @17:02:38.13 Carousel.home.seek 56 @17:02:38.45 Plunge.seek :locked @17:02:38.63 Archive.in :unlocking @17:02:38.77 Archive.seek :clear @17:02:38.83 Air.in :unlocking @17:02:39.01 Air.seek :clear @17:02:39.13 Lysis1.in :unlocking @17:02:39.25 Lysis1.seek :clear @17:02:39.37 Lysis2.in :unlocking @17:02:39.47 Lysis2.seek :clear @17:03:00.11 Ended log spr24sep1915h.spr.bz2 @17:03:00.13
SPR.command "stats_off" @17:03:00.37 Gate.power :analytic1,:OFF @17:03:00.38 Gate.power :motor,:OFF @17:03:00.39 Gate.power :core,:OFF @17:03:00.41 Gate.power :main,:OFF @17:03:00.42 Safely stopped and ready to power off @17:03:01.12 Gate.power :sampler,:OFF,limit to 3500mA @17:03:01.13 Gate.power :skimmer,:OFF,limit to 3500mA @17:03:04.22 Gate.power :main,:ON,limit to 5000mA @17:03:04.23 Gate.power :analytic1,:ON,limit to 4000mA @17:03:04.30 Powering core bus @17:03:06.67 SPR Module 1, FW Rev. B, FPGA Rev. C @17:03:07.66
Plunge.configure :default @17:03:07.74 SamplePump.configure :default @17:03:07.82 Toroid.configure :default @17:03:07.90 Carousel.configure :default @17:03:07.97 Abs.configure :default @17:03:08.05 PhaseSeparator.configure :default @17:03:08.13 Archive.configure :default @17:03:08.21 Air.configure :default @17:03:08.26 Lysis1.configure :default @17:03:08.32 Lysis2.configure :default @17:03:08.39 PinPort.configure :default @17:03:08.44 All elf controllers running firmware version 4.92 @17:03:08.47 Gate.power :motor,:ON,limit to 5000mA @17:03:08.79 Plunge.home.jog 0 @17:03:09.43 Lysis2.home.jog 0 @17:03:09.51 Air.home.jog 0 @17:03:09.54 Carousel.home.jog 0 @17:03:09.61 Archive.home.jog 0 @17:03:09.66 Toroid.home.jog 0 @17:03:09.69 Lysis1.home.jog 0 @17:03:09.72 PinPort.home.jog 0 @17:03:10.51 Plunge.in :homing @17:03:10.78 Plunge.jog 1600 @17:03:10.97 Lysis2.in :homing @17:03:11.03 Lysis1.in :homing @17:03:11.07 Archive.in :homing @17:03:11.08 PinPort.in :homing @17:03:11.12 Air.in :homing @17:03:11.14 Toroid.in :homing @17:03:11.38 Lysis1.jog 1399 @17:03:11.46 PinPort.jog -112 @17:03:11.51 Lysis2.jog 1406 @17:03:11.53 Archive.jog 1398 @17:03:11.57 Air.jog 1377 @17:03:11.62 Toroid.jog 627 @17:03:12.06 Plunge.setRawPosition! 50 @17:03:12.08 PinPort.setRawPosition! 0 @17:03:12.19 PinPort.seek :home @17:03:12.21 Plunge.seek :home @17:03:12.41 PinPort.in :default @17:03:12.58 Plunge.in :default @17:03:12.79 Lysis1.setRawPosition! 50 @17:03:12.84 Lysis1.seek :home @17:03:12.93 Lysis2.setRawPosition! 50 @17:03:12.98 Lysis2.seek :home @17:03:13.12 Lysis1.in :default @17:03:13.15 Air.setRawPosition! 50 @17:03:13.17 Archive.setRawPosition! 50 @17:03:13.32 Air.seek :home @17:03:13.36 Archive.seek :home @17:03:13.56 Lysis2.in :default @17:03:13.70 Air.in :default @17:03:13.79 Archive.in :default @17:03:14.03 Toroid.setRawPosition! 0 @17:03:14.09 Toroid.seek :home @17:03:14.19 Toroid.in :default @17:03:14.69 Toroid.seek :clear @17:03:16.44
Carousel.in :default @17:03:16.56 Carousel.home.seek 56 @17:03:16.83 Selecting Cartridge 55 @17:03:17.11 Carousel.seek 55 @17:03:17.64 Lysis1.in :fast @17:03:17.69 Lysis1.seek :locked @17:03:20.31 Plunge.seek :locked @17:03:20.47 Air.in :fast @17:03:20.53 Air.seek :locked @17:03:21.50
Carousel.reconfigure :locked @17:03:21.65 Powering cartridge bus @17:03:24.30 Gate.setIR 1750,5000,0,6 @17:03:24.42 Gate.setIR 0,5000,0,6 @17:03:24.49 PuckHeater.configure :default @17:03:24.55 Gate.power :heat,:ON,limit to 5000mA @17:03:25.16 Mounted lysis Cartridge @17:03:25.19 WARNING: Using default puck temperature sensor calibration @17:03:25.21 Using default cartridge heat loss curve for HeatingAccelInCarousel /home/esp/esp2/lib/thread.rb:984: [BUG] Segmentation fault ruby 1.8.9 (2021-11-29 MBARI8esp8/0x8770 on patchlevel 352) [arm-linux-eabi] Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @11:15:29.35PDT23-Sep-24
Environmental Sample Processor built 01-Jul-24 (3743e013) @11:15:31.39 >BREAK< ESP3 Gateway v1.43 for core bus @11:15:31.42 >BREAK< ESP3 Gateway v1.43 for cartridge bus @11:15:34.93 Configuration for honu by nadia@mbari.org 8/14/24 /home/esp/esp2/type/auv/honu/configure.rb @11:15:35.61 Sea pressure (Abs) tare is 6% of full scale @11:15:39.26 Polling Can every 2:00 @11:15:39.31 Loading /home/esp/esp2/bin/processAll mission @11:15:39.53 Sea pressure (Abs) tare is 6% of full scale @11:15:39.55 Sampling default goal volumes @11:15:39.57 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @11:15:39.73 Gate.power :sampler,:OFF,limit to 3500mA @11:15:39.74 Gate.power :skimmer,:OFF,limit to 3500mA @11:15:39.76 Gate.power :analytic1,:ON,limit to 4000mA @11:15:40.42 Session Begun @11:15:40.63 SPR Module 1, FW Rev. B, FPGA Rev. C @11:15:41.03
Plunge.configure :default @11:15:41.21 SamplePump.configure :default @11:15:41.37 Toroid.configure :default @11:15:41.45 Toroid.hold @11:15:41.55 Toroid.configure :default @11:15:41.71 Carousel.configure :default @11:15:41.78 Carousel.hold @11:15:41.88 Carousel.configure :default @11:15:42.07 Abs.configure :default @11:15:42.24 PhaseSeparator.configure :default @11:15:42.41 Archive.configure :default @11:15:42.57 Air.configure :default @11:15:42.71 Lysis1.configure :default @11:15:42.85 Lysis2.configure :default @11:15:43.02 PinPort.configure :default @11:15:43.10 All elf controllers running firmware version 4.92 @11:15:43.93 Toroid.seek :clear @11:15:44.16
Carousel.in :default @11:15:44.30 Carousel.home.seek 55 @11:15:44.60 Selecting Cartridge 55 @11:15:44.92 Carousel.seek 55 @11:15:45.05 Gate.power :heat,:OFF @11:15:45.08 Gate.power :cartridge,:OFF @11:15:45.29 Lysis1.in :fast @11:15:45.37 Lysis1.seek :locked @11:15:48.12 Plunge.seek :locked @11:15:48.32 Air.in :fast @11:15:48.40 Air.seek :locked @11:15:49.40
Carousel.reconfigure :locked @11:15:49.58 Powering cartridge bus @11:15:53.23 Gate.setIR 1750,5000,0,6 @11:15:53.36 Gate.setIR 0,5000,0,6 @11:15:53.45 PuckHeater.configure :default @11:15:53.54 Gate.power :heat,:ON,limit to 5000mA @11:15:54.21 Mounted lysis Cartridge @11:15:54.25 WARNING: Using default puck temperature sensor calibration @11:15:54.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:15:55.88 SPR.command "read_sequences" @11:15:56.50 SPR.command "stats_on" @11:15:56.68 SPR.command "start 0 1" @11:15:57.39 SPR.start /var/log/esp/spr24sep2311h.spr.bz2 @11:15:59.36 Waiting for baseline SPR stabilization @11:15:59.38 Handoff.select :waste @11:15:59.56 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:16:06.21 Syringe tare is 52% of full scale at 22C (drifted 1%) @11:16:06.27 PinPort.in :locking @11:16:06.35 PinPort.seek :locked @11:16:06.87 Archive.in :fast @11:16:06.96 Lysis2.in :fast @11:16:07.06 Archive.seek :full @11:16:07.12 Lysis1.seek 1100uL @11:16:07.25 Lysis2.seek 1100uL @11:16:11.56 delayUntil "11:25:58.91" @11:22:23.13 Session Begun @11:25:58.92 Lysis2.in :DAmix @11:25:59.09 Lysis1.in :DAmix @11:25:59.70 Lysis2.seek 950uL @11:25:59.81 Lysis1.seek 950uL @11:26:20.64 delay 5 seconds @11:26:25.67 Handoff.select SPR @11:26:25.85 SPR.command "start 2 14" @11:26:26.44 SPR stabilized @11:26:26.65 delay 10 seconds @11:26:36.67 SPR.command "start 2 0" @11:26:39.81 Waiting up to 11:00 for SPR to enter state 1 @11:28:37.48 SPR.command "hold_on" @11:28:38.87 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @11:28:39.53 Lysis2.seek 500uL @11:28:39.59 Lysis1.seek 500uL @11:29:41.80 delay 15 seconds @11:29:56.84 SPR.command "hold_off" @11:30:00.33 Waiting up to 1:00:00 for SPR to enter state 7 @11:46:15.89 SPRsummary:44.6RIU,176RIU,351RIU,78.4RIU after 1920s @12:00:32.93 delay 10 seconds @12:00:42.96 SPR.command "start 2 0" @12:00:46.17 Waiting up to 11:00 for SPR to enter state 1 @12:02:44.27 SPR.command "hold_on" @12:02:45.65 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:02:46.73 Lysis2.seek 275uL @12:02:46.84 Lysis1.seek 275uL @12:03:18.02 delay 15 seconds @12:03:33.05 SPR.command "hold_off" @12:03:35.90 Waiting up to 1:00:00 for SPR to enter state 7 @12:34:08.51 delay 10 seconds @12:34:18.53 SPR.command "start 2 0" @12:34:21.22 Waiting up to 11:00 for SPR to enter state 1 @12:36:18.87 SPR.command "hold_on" @12:36:20.27 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @12:36:20.96 Lysis2.seek 50uL @12:36:21.07 Lysis1.seek 50uL @12:36:52.23 delay 15 seconds @12:37:07.25 SPR.command "hold_off" @12:37:10.52 Waiting up to 1:00:00 for SPR to enter state 7 @13:07:43.05 Additional SPR Analyses Skipped due to Insufficient Volume @13:07:43.30 Intake.close @13:07:43.37 Exhaust.close @13:07:43.45 Bypass.close @13:07:43.63 Handoff.select :close @13:07:43.99
Gate.power :heat,:OFF @13:07:44.04 Gate.power :cartridge,:OFF @13:07:44.22 PinPort.in :unlocking @13:07:44.32 PinPort.seek :clear @13:07:44.86 Carousel.in :default @13:07:45.07 Carousel.home.seek 55 @13:07:45.44 Plunge.seek :locked @13:07:45.68 Archive.in :unlocking @13:07:45.89 Archive.seek :clear @13:07:46.09 Air.in :unlocking @13:07:46.27 Air.seek :clear @13:07:46.44 Lysis1.in :unlocking @13:07:46.62 Lysis1.seek :clear @13:07:46.81 Lysis2.in :unlocking @13:07:46.93 Lysis2.seek :clear @13:08:07.69 Ended log spr24sep2311h.spr.bz2 @13:08:07.71
SPR.command "stats_off" @13:08:08.18 Gate.power :analytic1,:OFF @13:08:08.21 Gate.power :motor,:OFF @13:08:08.26 Gate.power :core,:OFF @13:08:08.31 Gate.power :main,:OFF @13:08:08.34 Safely stopped and ready to power off @13:08:09.16 Gate.power :sampler,:OFF,limit to 3500mA @13:08:09.18 Gate.power :skimmer,:OFF,limit to 3500mA @13:08:12.12 Gate.power :main,:ON,limit to 5000mA @13:08:12.14 Gate.power :analytic1,:ON,limit to 4000mA @13:08:12.29 Powering core bus @13:08:14.61 SPR Module 1, FW Rev. B, FPGA Rev. C @13:08:15.07
Plunge.configure :default @13:08:15.75 SamplePump.configure :default @13:08:15.88 Toroid.configure :default @13:08:16.03 Carousel.configure :default @13:08:16.15 Abs.configure :default @13:08:16.28 PhaseSeparator.configure :default @13:08:16.40 Archive.configure :default @13:08:16.52 Air.configure :default @13:08:16.63 Lysis1.configure :default @13:08:16.73 Lysis2.configure :default @13:08:16.85 PinPort.configure :default @13:08:16.95 All elf controllers running firmware version 4.92 @13:08:16.98 Gate.power :motor,:ON,limit to 5000mA @13:08:17.25 Plunge.home.jog 0 @13:08:18.08 PinPort.home.jog 0 @13:08:18.13 Lysis1.home.jog 0 @13:08:18.20 Archive.home.jog 0 @13:08:18.29 Toroid.home.jog 0 @13:08:18.35 Lysis2.home.jog 0 @13:08:18.42 Carousel.home.jog 0 @13:08:18.47 Air.home.jog 0 @13:08:19.27 Plunge.in :homing @13:08:19.69 Plunge.jog 1601 @13:08:19.93 Lysis2.in :homing @13:08:20.01 Lysis1.in :homing @13:08:20.07 Archive.in :homing @13:08:20.15 PinPort.in :homing @13:08:20.20 Air.in :homing @13:08:20.27 Toroid.in :homing @13:08:20.52 Lysis2.jog 1406 @13:08:20.71 Lysis1.jog 1399 @13:08:20.75 Air.jog 1380 @13:08:20.83 Archive.jog 1399 @13:08:20.88 PinPort.jog -112 @13:08:20.97 Toroid.jog 626 @13:08:21.47 Plunge.setRawPosition! 50 @13:08:21.58 Plunge.seek :home @13:08:21.72 PinPort.setRawPosition! 0 @13:08:21.82 PinPort.seek :home @13:08:21.99 PinPort.in :default @13:08:22.03 Plunge.in :default @13:08:22.28 Lysis2.setRawPosition! 50 @13:08:22.39 Lysis2.seek :home @13:08:22.57 Lysis1.setRawPosition! 50 @13:08:22.66 Air.setRawPosition! 50 @13:08:22.74 Archive.setRawPosition! 50 @13:08:22.84 Lysis1.seek :home @13:08:22.96 Air.seek :home @13:08:23.06 Archive.seek :home @13:08:23.37 Lysis1.in :default @13:08:23.40 Lysis2.in :default @13:08:23.65 Archive.in :default @13:08:23.69 Toroid.setRawPosition! 0 @13:08:23.75 Air.in :default @13:08:23.97 Toroid.seek :home @13:08:24.16 Toroid.in :default @13:08:24.34 Toroid.seek :clear @13:08:26.10
Carousel.in :default @13:08:26.68 Carousel.home.seek 55 @13:08:27.10 Selecting Cartridge 54 @13:08:27.48 Carousel.seek 54 @13:08:28.08 Lysis1.in :fast @13:08:28.17 Lysis1.seek :locked @13:08:30.85 Plunge.seek :locked @13:08:31.06 Air.in :fast @13:08:31.17 Air.seek :locked @13:08:32.21
Carousel.reconfigure :locked @13:08:32.44 Powering cartridge bus @13:08:35.10 Gate.setIR 1750,5000,0,6 @13:08:35.23 Gate.setIR 0,5000,0,6 @13:08:35.35 PuckHeater.configure :default @13:08:35.46 Gate.power :heat,:ON,limit to 5000mA @13:08:36.12 Mounted lysis Cartridge @13:08:36.21 WARNING: Using default puck temperature sensor calibration @13:08:36.23 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:08:37.89 SPR.command "read_sequences" @13:08:38.16 SPR.command "stats_on" @13:08:38.35 SPR.command "start 0 1" @13:08:39.06 SPR.start /var/log/esp/spr24sep2313h.spr.bz2 @13:08:41.53 Waiting for baseline SPR stabilization @13:08:41.55 Handoff.select :waste @13:08:41.77 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @13:08:48.41 Syringe tare is 52% of full scale at 22C (drifted 1%) @13:08:48.48 PinPort.in :locking @13:08:48.59 PinPort.seek :locked @13:08:49.14 Archive.in :fast @13:08:49.27 Lysis2.in :fast @13:08:49.37 Lysis1.seek 1100uL @13:08:49.49 Archive.seek :full @13:08:49.58 Lysis2.seek 1100uL @13:08:54.59 delayUntil "13:18:41.49" @13:18:41.51 Lysis2.in :DAmix @13:18:41.67 Lysis1.in :DAmix @13:18:42.36 Lysis2.seek 950uL @13:18:42.43 Lysis1.seek 950uL @13:19:03.35 delay 5 seconds @13:19:08.38 Handoff.select SPR @13:19:08.57 SPR.command "start 2 14" @13:19:10.04 SPR stabilized @13:19:10.24 delay 10 seconds @13:19:20.27 SPR.command "start 2 0" @13:19:23.36 Waiting up to 11:00 for SPR to enter state 1 @13:21:21.03 SPR.command "hold_on" @13:21:22.93 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @13:21:23.59 Lysis2.seek 500uL @13:21:23.68 Lysis1.seek 500uL @13:22:25.92 delay 15 seconds @13:22:40.94 SPR.command "hold_off" @13:22:43.87 Waiting up to 1:00:00 for SPR to enter state 7 @13:38:58.49 SPRsummary:-2.66RIU,84.6RIU,179RIU,-0.527RIU after 1921s @13:53:15.52 delay 10 seconds @13:53:25.55 SPR.command "start 2 0" @13:53:28.25 Waiting up to 11:00 for SPR to enter state 1 @13:55:25.92 SPR.command "hold_on" @13:55:27.30 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @13:55:28.42 Lysis2.seek 275uL @13:55:28.53 Lysis1.seek 275uL @13:55:59.73 delay 15 seconds @13:56:14.75 SPR.command "hold_off" @13:56:17.55 Waiting up to 1:00:00 for SPR to enter state 7 @14:26:50.19 delay 10 seconds @14:27:00.21 SPR.command "start 2 0" @14:27:02.86 Waiting up to 11:00 for SPR to enter state 1 @14:29:00.52 SPR.command "hold_on" @14:29:01.92 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @14:29:02.67 Lysis2.seek 50uL @14:29:02.73 Lysis1.seek 50uL @14:29:33.98 delay 15 seconds @14:29:49.04 SPR.command "hold_off" @14:29:52.17 Waiting up to 1:00:00 for SPR to enter state 7 @15:00:24.71 Additional SPR Analyses Skipped due to Insufficient Volume @15:00:24.96 Handoff.select :close @15:00:25.16
Gate.power :heat,:OFF @15:00:25.21 Gate.power :cartridge,:OFF @15:00:25.34 PinPort.in :unlocking @15:00:25.44 PinPort.seek :clear @15:00:25.97 Carousel.in :default @15:00:26.17 Carousel.home.seek 54 @15:00:26.59 Plunge.seek :locked @15:00:26.83 Archive.in :unlocking @15:00:27.03 Archive.seek :clear @15:00:27.10 Air.in :unlocking @15:00:27.35 Air.seek :clear @15:00:27.50 Lysis1.in :unlocking @15:00:27.73 Lysis1.seek :clear @15:00:27.85 Lysis2.in :unlocking @15:00:28.04 Lysis2.seek :clear @15:00:48.37 Ended log spr24sep2313h.spr.bz2 @15:00:48.39
SPR.command "stats_off" @15:00:48.89 Gate.power :analytic1,:OFF @15:00:48.91 Gate.power :motor,:OFF @15:00:48.97 Gate.power :core,:OFF @15:00:49.02 Gate.power :main,:OFF @15:00:49.08 Safely stopped and ready to power off @15:00:49.39 Gate.power :sampler,:OFF,limit to 3500mA @15:00:49.41 Gate.power :skimmer,:OFF,limit to 3500mA @15:00:52.83 Gate.power :main,:ON,limit to 5000mA @15:00:52.85 Gate.power :analytic1,:ON,limit to 4000mA @15:00:53.00 Powering core bus @15:00:55.32 SPR Module 1, FW Rev. B, FPGA Rev. C @15:00:56.35
Plunge.configure :default @15:00:56.47 SamplePump.configure :default @15:00:56.60 Toroid.configure :default @15:00:56.73 Carousel.configure :default @15:00:56.86 Abs.configure :default @15:00:56.99 PhaseSeparator.configure :default @15:00:57.11 Archive.configure :default @15:00:57.25 Air.configure :default @15:00:57.34 Lysis1.configure :default @15:00:57.45 Lysis2.configure :default @15:00:57.58 PinPort.configure :default @15:00:57.67 All elf controllers running firmware version 4.92 @15:00:57.70 Gate.power :motor,:ON,limit to 5000mA @15:00:58.16 Plunge.home.jog 0 @15:00:58.82 PinPort.home.jog 0 @15:00:58.89 Lysis2.home.jog 0 @15:00:58.95 Air.home.jog 0 @15:00:59.03 Carousel.home.jog 0 @15:00:59.09 Archive.home.jog 0 @15:00:59.15 Toroid.home.jog 0 @15:00:59.22 Lysis1.home.jog 0 @15:01:00.09 Plunge.in :homing @15:01:00.46 Plunge.jog 1603 @15:01:00.70 Lysis2.in :homing @15:01:00.76 Lysis1.in :homing @15:01:00.82 Archive.in :homing @15:01:00.89 PinPort.in :homing @15:01:00.95 Air.in :homing @15:01:01.00 Toroid.in :homing @15:01:01.28 Lysis2.jog 1404 @15:01:01.49 PinPort.jog -111 @15:01:01.66 Lysis1.jog 1400 @15:01:01.76 Archive.jog 1401 @15:01:01.82 Toroid.jog 626 @15:01:01.88 Air.jog 1379 @15:01:02.37 PinPort.setRawPosition! 0 @15:01:02.39 Plunge.setRawPosition! 50 @15:01:02.57 PinPort.seek :home @15:01:02.61 Plunge.seek :home @15:01:02.91 PinPort.in :default @15:01:02.99 Lysis2.setRawPosition! 50 @15:01:03.12 Lysis2.seek :home @15:01:03.28 Plunge.in :default @15:01:03.44 Lysis1.setRawPosition! 50 @15:01:03.47 Air.setRawPosition! 50 @15:01:03.54 Archive.setRawPosition! 50 @15:01:03.76 Lysis1.seek :home @15:01:03.81 Air.seek :home @15:01:03.85 Archive.seek :home @15:01:04.25 Lysis2.in :default @15:01:04.47 Air.in :default @15:01:04.50 Archive.in :default @15:01:04.58 Toroid.setRawPosition! 0 @15:01:04.67 Lysis1.in :default @15:01:04.93 Toroid.seek :home @15:01:05.08 Toroid.in :default @15:01:05.75 Toroid.seek :clear @15:01:07.46
Carousel.in :default @15:01:07.62 Carousel.home.seek 54 @15:01:07.93 Selecting Cartridge 53 @15:01:08.33 Carousel.seek 53 @15:01:08.92 Lysis1.in :fast @15:01:09.02 Lysis1.seek :locked @15:01:11.69 Plunge.seek :locked @15:01:11.90 Air.in :fast @15:01:12.04 Air.seek :locked @15:01:13.06
Carousel.reconfigure :locked @15:01:13.26 Powering cartridge bus @15:01:15.92 Gate.setIR 1750,5000,0,6 @15:01:16.06 Gate.setIR 0,5000,0,6 @15:01:16.17 PuckHeater.configure :default @15:01:16.29 Gate.power :heat,:ON,limit to 5000mA @15:01:16.92 Mounted lysis Cartridge @15:01:16.97 WARNING: Using default puck temperature sensor calibration @15:01:17.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:01:18.65 SPR.command "read_sequences" @15:01:18.87 SPR.command "stats_on" @15:01:19.06 SPR.command "start 0 1" @15:01:19.77 SPR.start /var/log/esp/spr24sep2315h.spr.bz2 @15:01:21.72 Waiting for baseline SPR stabilization @15:01:21.74 Handoff.select :waste @15:01:21.95 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:01:28.59 Syringe tare is 52% of full scale at 22C (drifted 1%) @15:01:28.63 PinPort.in :locking @15:01:28.76 PinPort.seek :locked @15:01:29.32 Archive.in :fast @15:01:30.02 Lysis2.in :fast @15:01:30.14 Lysis1.seek 1100uL @15:01:30.22 Archive.seek :full @15:01:30.48 Lysis2.seek 1100uL @15:01:34.77 delayUntil "15:11:21.28" @15:11:21.30 Lysis2.in :DAmix @15:11:21.45 Lysis1.in :DAmix @15:11:22.16 Lysis2.seek 950uL @15:11:22.20 Lysis1.seek 950uL @15:11:43.14 delay 5 seconds @15:11:48.17 Handoff.select SPR @15:11:48.36 SPR.command "start 2 14" @15:11:50.27 SPR stabilized @15:11:50.49 delay 10 seconds @15:12:00.51 SPR.command "start 2 0" @15:12:03.64 Waiting up to 11:00 for SPR to enter state 1 @15:14:01.72 SPR.command "hold_on" @15:14:03.64 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @15:14:04.32 Lysis2.seek 500uL @15:14:04.36 Lysis1.seek 500uL @15:15:06.66 delay 15 seconds @15:15:21.68 SPR.command "hold_off" @15:15:24.58 Waiting up to 1:00:00 for SPR to enter state 7 @15:31:39.20 SPRsummary:0.513RIU,35.4RIU,66.7RIU,3.1RIU after 1921s @15:45:56.24 delay 10 seconds @15:46:06.26 SPR.command "start 2 0" @15:46:08.95 Waiting up to 11:00 for SPR to enter state 1 @15:48:06.61 SPR.command "hold_on" @15:48:08.04 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @15:48:08.73 Lysis2.seek 275uL @15:48:08.80 Lysis1.seek 275uL @15:48:40.06 delay 15 seconds @15:48:55.08 SPR.command "hold_off" @15:48:58.25 Waiting up to 1:00:00 for SPR to enter state 7 @16:19:30.87 delay 10 seconds @16:19:40.90 SPR.command "start 2 0" @16:19:43.58 Waiting up to 11:00 for SPR to enter state 1 @16:21:41.25 SPR.command "hold_on" @16:21:42.63 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @16:21:43.32 Lysis2.seek 50uL @16:21:43.36 Lysis1.seek 50uL @16:22:14.61 delay 15 seconds @16:22:29.63 SPR.command "hold_off" @16:22:32.88 Waiting up to 1:00:00 for SPR to enter state 7 @16:53:05.42 Additional SPR Analyses Skipped due to Insufficient Volume @16:53:05.68 Handoff.select :close @16:53:05.88
Gate.power :heat,:OFF @16:53:05.90 Gate.power :cartridge,:OFF @16:53:06.10 PinPort.in :unlocking @16:53:06.19 PinPort.seek :clear @16:53:06.74 Carousel.in :default @16:53:06.92 Carousel.home.seek 53 @16:53:07.35 Plunge.seek :locked @16:53:07.58 Archive.in :unlocking @16:53:07.75 Archive.seek :clear @16:53:07.89 Air.in :unlocking @16:53:08.10 Air.seek :clear @16:53:08.29 Lysis1.in :unlocking @16:53:08.46 Lysis1.seek :clear @16:53:08.63 Lysis2.in :unlocking @16:53:08.74 Lysis2.seek :clear @16:53:29.02 Ended log spr24sep2315h.spr.bz2 @16:53:29.05
SPR.command "stats_off" @16:53:29.56 Gate.power :analytic1,:OFF @16:53:29.58 Gate.power :motor,:OFF @16:53:29.64 Gate.power :core,:OFF @16:53:29.70 Gate.power :main,:OFF @16:53:29.75 Safely stopped and ready to power off @16:53:30.06 Gate.power :sampler,:OFF,limit to 3500mA @16:53:30.09 Gate.power :skimmer,:OFF,limit to 3500mA @16:53:33.51 Gate.power :main,:ON,limit to 5000mA @16:53:33.53 Gate.power :analytic1,:ON,limit to 4000mA @16:53:33.68 Powering core bus @16:53:36.05 SPR Module 1, FW Rev. B, FPGA Rev. C @16:53:36.47
Plunge.configure :default @16:53:36.59 SamplePump.configure :default @16:53:36.71 Toroid.configure :default @16:53:36.84 Carousel.configure :default @16:53:36.96 Abs.configure :default @16:53:37.08 PhaseSeparator.configure :default @16:53:37.20 Archive.configure :default @16:53:37.32 Air.configure :default @16:53:37.41 Lysis1.configure :default @16:53:37.50 Lysis2.configure :default @16:53:37.63 PinPort.configure :default @16:53:37.72 All elf controllers running firmware version 4.92 @16:53:37.76 Gate.power :motor,:ON,limit to 5000mA @16:53:38.18 Plunge.home.jog 0 @16:53:39.42 Lysis2.home.jog 0 @16:53:39.46 Lysis1.home.jog 0 @16:53:39.53 Air.home.jog 0 @16:53:39.59 Carousel.home.jog 0 @16:53:39.68 PinPort.home.jog 0 @16:53:39.75 Archive.home.jog 0 @16:53:39.82 Toroid.home.jog 0 @16:53:40.75 Plunge.in :homing @16:53:41.10 Plunge.jog 1597 @16:53:41.40 Toroid.in :homing @16:53:41.49 Lysis2.in :homing @16:53:41.57 Air.in :homing @16:53:41.63 Lysis1.in :homing @16:53:41.69 PinPort.in :homing @16:53:41.77 Archive.in :homing @16:53:42.08 Toroid.jog 630 @16:53:42.19 Lysis2.jog 1405 @16:53:42.26 Air.jog 1382 @16:53:42.32 Lysis1.jog 1399 @16:53:42.39 PinPort.jog -111 @16:53:42.53 Archive.jog 1400 @16:53:42.94 Plunge.setRawPosition! 50 @16:53:43.05 Plunge.seek :home @16:53:43.20 PinPort.setRawPosition! 0 @16:53:43.29 PinPort.seek :home @16:53:43.43 Plunge.in :default @16:53:43.55 PinPort.in :default @16:53:43.72 Lysis2.setRawPosition! 50 @16:53:43.84 Air.setRawPosition! 50 @16:53:43.88 Lysis2.seek :home @16:53:44.13 Air.seek :home @16:53:44.28 Lysis1.setRawPosition! 50 @16:53:44.32 Archive.setRawPosition! 50 @16:53:44.53 Lysis1.seek :home @16:53:44.56 Archive.seek :home @16:53:44.84 Lysis2.in :default @16:53:44.85 Air.in :default @16:53:44.93 Toroid.setRawPosition! 0 @16:53:45.14 Toroid.seek :home @16:53:45.28 Archive.in :default @16:53:45.33 Lysis1.in :default @16:53:45.54 Toroid.in :default @16:53:45.77 Toroid.seek :clear @16:53:47.52
Carousel.in :default @16:53:47.68 Carousel.home.seek 53 @16:53:48.00 Selecting Cartridge 52 @16:53:48.36 Carousel.seek 52 @16:53:48.93 Lysis1.in :fast @16:53:49.03 Lysis1.seek :locked @16:53:52.23 Plunge.seek :locked @16:53:52.46 Air.in :fast @16:53:52.55 Air.seek :locked @16:53:53.58
Carousel.reconfigure :locked @16:53:53.78 Powering cartridge bus @16:53:56.44 Gate.setIR 1750,5000,0,6 @16:53:56.57 Gate.setIR 0,5000,0,6 @16:53:56.68 PuckHeater.configure :default @16:53:56.79 Gate.power :heat,:ON,limit to 5000mA @16:53:57.44 Mounted lysis Cartridge @16:53:57.49 WARNING: Using default puck temperature sensor calibration @16:53:57.54 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:53:59.23 SPR.command "read_sequences" @16:53:59.55 SPR.command "stats_on" @16:53:59.74 SPR.command "start 0 1" @16:54:00.45 SPR.start /var/log/esp/spr24sep2316h.spr.bz2 @16:54:02.40 Waiting for baseline SPR stabilization @16:54:02.42 Handoff.select :waste @16:54:02.65 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @16:54:09.29 Syringe tare is 52% of full scale at 22C (drifted 1%) @16:54:09.33 PinPort.in :locking @16:54:09.45 PinPort.seek :locked @16:54:10.06 Archive.in :fast @16:54:10.18 Lysis2.in :fast @16:54:10.27 Lysis1.seek 1100uL @16:54:10.34 Archive.seek :full @16:54:10.59 Lysis2.seek 1100uL @16:54:14.93 delayUntil "17:04:01.96" @17:04:01.98 Lysis2.in :DAmix @17:04:02.17 Lysis1.in :DAmix @17:04:02.81 Lysis2.seek 950uL @17:04:02.86 Lysis1.seek 950uL @17:04:23.80 delay 5 seconds @17:04:28.82 Handoff.select SPR @17:04:29.01 SPR.command "start 2 14" @17:04:30.43 SPR stabilized @17:04:30.63 delay 10 seconds @17:04:40.66 SPR.command "start 2 0" @17:04:43.80 Waiting up to 11:00 for SPR to enter state 1 @17:06:41.47 SPR.command "hold_on" @17:06:43.37 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @17:06:44.04 Lysis2.seek 500uL @17:06:44.12 Lysis1.seek 500uL @17:07:46.40 delay 15 seconds @17:08:01.42 SPR.command "hold_off" @17:08:04.83 Waiting up to 1:00:00 for SPR to enter state 7 @17:24:19.88 SPRsummary:0.665RIU,20.5RIU,44.4RIU,1.22RIU after 1921s @17:38:36.92 delay 10 seconds @17:38:46.95 SPR.command "start 2 0" @17:38:49.62 Waiting up to 11:00 for SPR to enter state 1 @17:40:47.30 SPR.command "hold_on" @17:40:48.69 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @17:40:49.44 Lysis2.seek 275uL @17:40:49.48 Lysis1.seek 275uL @17:41:20.72 delay 15 seconds @17:41:35.74 SPR.command "hold_off" @17:41:38.94 Waiting up to 1:00:00 for SPR to enter state 7 @18:12:11.54 delay 10 seconds @18:12:21.56 SPR.command "start 2 0" @18:12:24.26 Waiting up to 11:00 for SPR to enter state 1 @18:14:21.92 SPR.command "hold_on" @18:14:23.31 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @18:14:24.05 Lysis2.seek 50uL @18:14:24.10 Lysis1.seek 50uL @18:14:55.36 delay 15 seconds @18:15:10.39 SPR.command "hold_off" @18:15:13.56 Waiting up to 1:00:00 for SPR to enter state 7 @18:45:46.11 Additional SPR Analyses Skipped due to Insufficient Volume @18:45:46.38 Handoff.select :close @18:45:46.58
Gate.power :heat,:OFF @18:45:46.61 Gate.power :cartridge,:OFF @18:45:46.77 PinPort.in :unlocking @18:45:46.86 PinPort.seek :clear @18:45:47.41 Carousel.in :default @18:45:47.57 Carousel.home.seek 52 @18:45:48.03 Plunge.seek :locked @18:45:48.29 Archive.in :unlocking @18:45:48.48 Archive.seek :clear @18:45:48.61 Air.in :unlocking @18:45:48.82 Air.seek :clear @18:45:48.90 Lysis1.in :unlocking @18:45:49.15 Lysis1.seek :clear @18:45:49.31 Lysis2.in :unlocking @18:45:49.42 Lysis2.seek :clear @18:46:09.62 Ended log spr24sep2316h.spr.bz2 @18:46:09.65
SPR.command "stats_off" @18:46:10.24 Gate.power :analytic1,:OFF @18:46:10.26 Gate.power :motor,:OFF @18:46:10.34 Gate.power :core,:OFF @18:46:10.36 Gate.power :main,:OFF @18:46:10.39 Safely stopped and ready to power off @18:46:10.64 Gate.power :sampler,:OFF,limit to 3500mA @18:46:10.67 Gate.power :skimmer,:OFF,limit to 3500mA @18:46:14.17 Gate.power :main,:ON,limit to 5000mA @18:46:14.19 Gate.power :analytic1,:ON,limit to 4000mA @18:46:14.33 Powering core bus @18:46:16.67 SPR Module 1, FW Rev. B, FPGA Rev. C @18:46:17.36
Plunge.configure :default @18:46:17.48 SamplePump.configure :default @18:46:17.60 Toroid.configure :default @18:46:17.71 Carousel.configure :default @18:46:17.83 Abs.configure :default @18:46:17.95 PhaseSeparator.configure :default @18:46:18.10 Archive.configure :default @18:46:18.22 Air.configure :default @18:46:18.32 Lysis1.configure :default @18:46:18.41 Lysis2.configure :default @18:46:18.52 PinPort.configure :default @18:46:18.61 All elf controllers running firmware version 4.92 @18:46:18.67 Gate.power :motor,:ON,limit to 5000mA @18:46:19.06 Plunge.home.jog 0 @18:46:19.76 PinPort.home.jog 0 @18:46:19.80 Lysis1.home.jog 0 @18:46:19.84 Archive.home.jog 0 @18:46:19.91 Toroid.home.jog 0 @18:46:20.00 Lysis2.home.jog 0 @18:46:20.10 Carousel.home.jog 0 @18:46:20.17 Air.home.jog 0 @18:46:21.09 Plunge.in :homing @18:46:21.45 Plunge.jog 1596 @18:46:21.55 Lysis2.in :homing @18:46:21.61 Air.in :homing @18:46:21.68 Toroid.in :homing @18:46:21.75 PinPort.in :homing @18:46:21.81 Lysis1.in :homing @18:46:21.87 Archive.in :homing @18:46:22.19 Lysis2.jog 1404 @18:46:22.35 Air.jog 1376 @18:46:22.50 PinPort.jog -111 @18:46:22.57 Lysis1.jog 1400 @18:46:22.61 Archive.jog 1400 @18:46:22.67 Toroid.jog 630 @18:46:23.14 PinPort.setRawPosition! 0 @18:46:23.25 PinPort.seek :home @18:46:23.41 Plunge.setRawPosition! 50 @18:46:23.44 PinPort.in :default @18:46:23.59 Plunge.seek :home @18:46:23.76 Lysis2.setRawPosition! 50 @18:46:23.85 Lysis2.seek :home @18:46:23.98 Plunge.in :default @18:46:24.23 Lysis1.setRawPosition! 50 @18:46:24.27 Air.setRawPosition! 50 @18:46:24.32 Archive.setRawPosition! 50 @18:46:24.52 Lysis1.seek :home @18:46:24.58 Air.seek :home @18:46:25.12 Archive.seek :home @18:46:25.52 Lysis2.in :default @18:46:25.55 Lysis1.in :default @18:46:25.63 Toroid.setRawPosition! 0 @18:46:25.86 Toroid.seek :home @18:46:26.07 Archive.in :default @18:46:26.10 Toroid.in :default @18:46:26.15 Air.in :default @18:46:26.66 Toroid.seek :clear @18:46:28.40
Carousel.in :default @18:46:28.56 Carousel.home.seek 52 @18:46:28.87 Selecting Cartridge 51 @18:46:29.21 Carousel.seek 51 @18:46:29.79 Lysis1.in :fast @18:46:29.88 Lysis1.seek :locked @18:46:32.55 Plunge.seek :locked @18:46:32.77 Air.in :fast @18:46:32.87 Air.seek :locked @18:46:33.89
Carousel.reconfigure :locked @18:46:34.11 Powering cartridge bus @18:46:36.77 Gate.setIR 1750,5000,0,6 @18:46:36.91 Gate.setIR 0,5000,0,6 @18:46:37.01 PuckHeater.configure :default @18:46:37.12 Gate.power :heat,:ON,limit to 5000mA @18:46:37.74 Mounted lysis Cartridge @18:46:37.78 WARNING: Using default puck temperature sensor calibration @18:46:37.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:46:39.51 SPR.command "read_sequences" @18:46:40.19 SPR.command "stats_on" @18:46:40.39 SPR.command "start 0 1" @18:46:41.10 SPR.start /var/log/esp/spr24sep2318h.spr.bz2 @18:46:43.05 Waiting for baseline SPR stabilization @18:46:43.07 Handoff.select :waste @18:46:43.28 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @18:46:49.91 Syringe tare is 52% of full scale at 22C (drifted 1%) @18:46:49.97 PinPort.in :locking @18:46:50.10 PinPort.seek :locked @18:46:50.68 Archive.in :fast @18:46:50.81 Lysis2.in :fast @18:46:50.92 Lysis1.seek 1100uL @18:46:50.97 Archive.seek :full @18:46:51.19 Lysis2.seek 1100uL @18:46:55.54 delayUntil "18:56:42.61" @18:56:42.63 Lysis2.in :DAmix @18:56:42.79 Lysis1.in :DAmix @18:56:43.36 Lysis2.seek 950uL @18:56:43.42 Lysis1.seek 950uL @18:57:04.37 delay 5 seconds @18:57:09.39 Handoff.select SPR @18:57:09.57 SPR.command "start 2 14" @18:57:10.13 SPR stabilized @18:57:10.34 delay 10 seconds @18:57:20.36 SPR.command "start 2 0" @18:57:23.51 Waiting up to 11:00 for SPR to enter state 1 @18:59:21.17 SPR.command "hold_on" @18:59:22.56 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @18:59:23.23 Lysis2.seek 500uL @18:59:23.30 Lysis1.seek 500uL @19:00:25.59 delay 15 seconds @19:00:40.62 SPR.command "hold_off" @19:00:44.04 Waiting up to 1:00:00 for SPR to enter state 7 @19:01:25.64 Session Ended @19:16:59.58 SPRsummary:-1.1RIU,11.1RIU,31.5RIU,3.64RIU after 1920s @19:31:16.61 delay 10 seconds @19:31:26.64 SPR.command "start 2 0" @19:31:29.34 Waiting up to 11:00 for SPR to enter state 1 @19:33:27.01 SPR.command "hold_on" @19:33:28.39 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @19:33:29.11 Lysis2.seek 275uL @19:33:29.17 Lysis1.seek 275uL @19:34:00.39 delay 15 seconds @19:34:15.41 SPR.command "hold_off" @19:34:18.64 Waiting up to 1:00:00 for SPR to enter state 7 @20:04:51.25 delay 10 seconds @20:05:01.27 SPR.command "start 2 0" @20:05:03.97 Waiting up to 11:00 for SPR to enter state 1 @20:07:01.63 SPR.command "hold_on" @20:07:03.01 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @20:07:03.71 Lysis2.seek 50uL @20:07:03.80 Lysis1.seek 50uL @20:07:34.95 delay 15 seconds @20:07:49.97 SPR.command "hold_off" @20:07:53.26 Waiting up to 1:00:00 for SPR to enter state 7 @20:38:25.80 Additional SPR Analyses Skipped due to Insufficient Volume @20:38:26.08 Handoff.select :close @20:38:26.27
Gate.power :heat,:OFF @20:38:26.29 Gate.power :cartridge,:OFF @20:38:26.42 PinPort.in :unlocking @20:38:26.51 PinPort.seek :clear @20:38:27.01 Carousel.in :default @20:38:27.16 Carousel.home.seek 51 @20:38:27.52 Plunge.seek :locked @20:38:27.78 Archive.in :unlocking @20:38:27.93 Archive.seek :clear @20:38:28.10 Air.in :unlocking @20:38:28.27 Air.seek :clear @20:38:28.42 Lysis1.in :unlocking @20:38:28.59 Lysis1.seek :clear @20:38:28.76 Lysis2.in :unlocking @20:38:28.85 Lysis2.seek :clear @20:38:49.28 Ended log spr24sep2318h.spr.bz2 @20:38:49.30
SPR.command "stats_off" @20:38:49.94 Gate.power :analytic1,:OFF @20:38:49.96 Gate.power :motor,:OFF @20:38:50.02 Gate.power :core,:OFF @20:38:50.06 Gate.power :main,:OFF @20:38:50.09 Safely stopped and ready to power off @20:38:50.30 Gate.power :sampler,:OFF,limit to 3500mA @20:38:50.33 Gate.power :skimmer,:OFF,limit to 3500mA @20:38:53.87 Gate.power :main,:ON,limit to 5000mA @20:38:53.89 Gate.power :analytic1,:ON,limit to 4000mA @20:38:54.04 Powering core bus @20:38:56.35 SPR Module 1, FW Rev. B, FPGA Rev. C @20:38:56.82
Plunge.configure :default @20:38:56.92 SamplePump.configure :default @20:38:57.02 Toroid.configure :default @20:38:57.12 Carousel.configure :default @20:38:57.22 Abs.configure :default @20:38:57.33 PhaseSeparator.configure :default @20:38:57.43 Archive.configure :default @20:38:57.54 Air.configure :default @20:38:57.60 Lysis1.configure :default @20:38:57.67 Lysis2.configure :default @20:38:57.77 PinPort.configure :default @20:38:57.86 All elf controllers running firmware version 4.92 @20:38:57.88 Gate.power :motor,:ON,limit to 5000mA @20:38:58.24 Plunge.home.jog 0 @20:38:58.93 PinPort.home.jog 0 @20:38:58.97 Lysis1.home.jog 0 @20:38:59.04 Archive.home.jog 0 @20:38:59.08 Toroid.home.jog 0 @20:38:59.14 Lysis2.home.jog 0 @20:38:59.18 Carousel.home.jog 0 @20:38:59.24 Air.home.jog 0 @20:39:00.28 Plunge.in :homing @20:39:00.54 Plunge.jog 1601 @20:39:00.63 Lysis2.in :homing @20:39:00.67 Air.in :homing @20:39:00.73 Toroid.in :homing @20:39:00.77 PinPort.in :homing @20:39:00.81 Archive.in :homing @20:39:00.87 Lysis1.in :homing @20:39:01.19 Lysis2.jog 1407 @20:39:01.23 Air.jog 1382 @20:39:01.27 Toroid.jog 630 @20:39:01.33 PinPort.jog -109 @20:39:01.41 Archive.jog 1400 @20:39:01.47 Lysis1.jog 1399 @20:39:01.93 PinPort.setRawPosition! 0 @20:39:02.04 PinPort.seek :home @20:39:02.19 Plunge.setRawPosition! 50 @20:39:02.22 PinPort.in :default @20:39:02.37 Plunge.seek :home @20:39:02.68 Plunge.in :default @20:39:02.81 Lysis2.setRawPosition! 50 @20:39:02.85 Air.setRawPosition! 50 @20:39:03.01 Lysis2.seek :home @20:39:03.02 Air.seek :home @20:39:03.69 Lysis1.setRawPosition! 50 @20:39:03.82 Archive.setRawPosition! 50 @20:39:03.86 Lysis2.in :default @20:39:04.03 Lysis1.seek :home @20:39:04.17 Archive.seek :home @20:39:04.36 Toroid.setRawPosition! 0 @20:39:04.38 Air.in :default @20:39:04.53 Toroid.seek :home @20:39:04.70 Lysis1.in :default @20:39:04.75 Archive.in :default @20:39:04.81 Toroid.in :default @20:39:05.19 Toroid.seek :clear @20:39:06.94
Carousel.in :default @20:39:07.10 Carousel.home.seek 51 @20:39:07.38 Selecting Cartridge 50 @20:39:07.71 Carousel.seek 50 @20:39:08.27 Lysis1.in :fast @20:39:08.36 Lysis1.seek :locked @20:39:11.01 Plunge.seek :locked @20:39:11.20 Air.in :fast @20:39:11.28 Air.seek :locked @20:39:12.29
Carousel.reconfigure :locked @20:39:12.46 Powering cartridge bus @20:39:15.12 Gate.setIR 1750,5000,0,6 @20:39:15.25 Gate.setIR 0,5000,0,6 @20:39:15.34 PuckHeater.configure :default @20:39:15.43 Gate.power :heat,:ON,limit to 5000mA @20:39:16.08 Mounted lysis Cartridge @20:39:16.14 WARNING: Using default puck temperature sensor calibration @20:39:16.16 Using default cartridge heat loss curve for HeatingAccelInCarousel @20:39:17.78 SPR.command "read_sequences" @20:39:18.01 SPR.command "stats_on" @20:39:18.20 SPR.command "start 0 1" @20:39:18.91 SPR.start /var/log/esp/spr24sep2320h.spr.bz2 @20:39:20.86 Waiting for baseline SPR stabilization @20:39:20.88 Handoff.select :waste @20:39:21.06 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @20:39:27.69 Syringe tare is 52% of full scale at 22C (drifted 1%) @20:39:27.73 PinPort.in :locking @20:39:27.83 PinPort.seek :locked @20:39:28.36 Archive.in :fast @20:39:28.47 Lysis2.in :fast @20:39:28.52 Lysis1.seek 1100uL @20:39:28.61 Archive.seek :full @20:39:28.77 Lysis2.seek 1100uL @20:39:33.14 delayUntil "20:49:20.41" @20:49:20.43 Lysis2.in :DAmix @20:49:20.57 Lysis1.in :DAmix @20:49:21.12 Lysis2.seek 950uL @20:49:21.19 Lysis1.seek 950uL @20:49:42.11 delay 5 seconds @20:49:47.14 Handoff.select SPR @20:49:47.30 SPR.command "start 2 14" @20:49:47.94 SPR stabilized @20:49:48.18 delay 10 seconds @20:49:58.20 SPR.command "start 2 0" @20:50:01.31 Waiting up to 11:00 for SPR to enter state 1 @20:51:58.98 SPR.command "hold_on" @20:52:00.36 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @20:52:01.03 Lysis2.seek 500uL @20:52:01.09 Lysis1.seek 500uL @20:53:03.37 delay 15 seconds @20:53:18.42 SPR.command "hold_off" @20:53:21.82 Waiting up to 1:00:00 for SPR to enter state 7 @21:09:37.39 SPRsummary:-1.57RIU,139RIU,283RIU,-2.38RIU after 1920s @21:23:54.44 delay 10 seconds @21:24:04.46 SPR.command "start 2 0" @21:24:07.14 Waiting up to 11:00 for SPR to enter state 1 @21:26:04.80 SPR.command "hold_on" @21:26:06.71 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @21:26:07.41 Lysis2.seek 275uL @21:26:07.46 Lysis1.seek 275uL @21:26:38.73 delay 15 seconds @21:26:53.75 SPR.command "hold_off" @21:26:56.44 Waiting up to 1:00:00 for SPR to enter state 7 @21:57:28.13 delay 10 seconds @21:57:38.15 SPR.command "start 2 0" @21:57:41.33 Waiting up to 11:00 for SPR to enter state 1 @21:59:39.43 SPR.command "hold_on" @21:59:40.81 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @21:59:41.48 Lysis2.seek 50uL @21:59:41.54 Lysis1.seek 50uL @22:00:12.77 delay 15 seconds @22:00:27.79 SPR.command "hold_off" @22:00:31.06 Waiting up to 1:00:00 for SPR to enter state 7 @22:31:03.60 Additional SPR Analyses Skipped due to Insufficient Volume @22:31:03.86 Handoff.select :close @22:31:04.05
Gate.power :heat,:OFF @22:31:04.07 Gate.power :cartridge,:OFF @22:31:04.20 PinPort.in :unlocking @22:31:04.28 PinPort.seek :clear @22:31:04.79 Carousel.in :default @22:31:04.94 Carousel.home.seek 50 @22:31:05.30 Plunge.seek :locked @22:31:05.56 Archive.in :unlocking @22:31:05.67 Archive.seek :clear @22:31:05.85 Air.in :unlocking @22:31:06.03 Air.seek :clear @22:31:06.18 Lysis1.in :unlocking @22:31:06.33 Lysis1.seek :clear @22:31:06.52 Lysis2.in :unlocking @22:31:06.63 Lysis2.seek :clear @22:31:27.50 Ended log spr24sep2320h.spr.bz2 @22:31:27.53
SPR.command "stats_off" @22:31:27.81 Gate.power :analytic1,:OFF @22:31:27.83 Gate.power :motor,:OFF @22:31:27.87 Gate.power :core,:OFF @22:31:27.91 Gate.power :main,:OFF @22:31:27.95 Safely stopped and ready to power off @22:31:28.26 Gate.power :sampler,:OFF,limit to 3500mA @22:31:28.29 Gate.power :skimmer,:OFF,limit to 3500mA @22:31:31.71 Gate.power :main,:ON,limit to 5000mA @22:31:31.74 Gate.power :analytic1,:ON,limit to 4000mA @22:31:31.84 Powering core bus @22:31:34.19 SPR Module 1, FW Rev. B, FPGA Rev. C @22:31:34.62
Plunge.configure :default @22:31:34.73 SamplePump.configure :default @22:31:35.39 Toroid.configure :default @22:31:35.49 Carousel.configure :default @22:31:35.59 Abs.configure :default @22:31:35.70 PhaseSeparator.configure :default @22:31:35.81 Archive.configure :default @22:31:35.91 Air.configure :default @22:31:35.98 Lysis1.configure :default @22:31:36.11 Lysis2.configure :default @22:31:36.22 PinPort.configure :default @22:31:36.30 All elf controllers running firmware version 4.92 @22:31:36.33 Gate.power :motor,:ON,limit to 5000mA @22:31:36.62 Plunge.home.jog 0 @22:31:37.40 PinPort.home.jog 0 @22:31:37.45 Lysis1.home.jog 0 @22:31:37.51 Archive.home.jog 0 @22:31:37.56 Toroid.home.jog 0 @22:31:37.62 Lysis2.home.jog 0 @22:31:37.66 Carousel.home.jog 0 @22:31:37.70 Air.home.jog 0 @22:31:38.54 Plunge.in :homing @22:31:38.89 Plunge.jog 1603 @22:31:39.11 Lysis2.in :homing @22:31:39.18 Lysis1.in :homing @22:31:39.23 Archive.in :homing @22:31:39.28 PinPort.in :homing @22:31:39.33 Toroid.in :homing @22:31:39.37 Air.in :homing @22:31:39.63 Lysis1.jog 1400 @22:31:39.70 Lysis2.jog 1406 @22:31:39.76 Archive.jog 1400 @22:31:39.81 PinPort.jog -111 @22:31:39.85 Toroid.jog 630 @22:31:39.90 Air.jog 1377 @22:31:40.40 Plunge.setRawPosition! 50 @22:31:40.48 Plunge.seek :home @22:31:40.60 PinPort.setRawPosition! 0 @22:31:40.68 PinPort.seek :home @22:31:40.85 Plunge.in :default @22:31:40.87 PinPort.in :default @22:31:41.17 Lysis1.setRawPosition! 50 @22:31:41.28 Lysis1.seek :home @22:31:41.43 Lysis2.setRawPosition! 50 @22:31:41.47 Air.setRawPosition! 50 @22:31:41.50 Archive.setRawPosition! 50 @22:31:41.71 Lysis2.seek :home @22:31:41.74 Air.seek :home @22:31:41.78 Archive.seek :home @22:31:42.15 Lysis1.in :default @22:31:42.18 Lysis2.in :default @22:31:42.41 Air.in :default @22:31:42.45 Archive.in :default @22:31:42.97 Toroid.setRawPosition! 0 @22:31:43.17 Toroid.seek :home @22:31:43.30 Toroid.in :default @22:31:43.46 Toroid.seek :clear @22:31:45.13
Carousel.in :default @22:31:45.28 Carousel.home.seek 50 @22:31:45.57 Selecting Cartridge 49 @22:31:45.89 Carousel.seek 49 @22:31:46.45 Lysis1.in :fast @22:31:46.52 Lysis1.seek :locked @22:31:49.17 Plunge.seek :locked @22:31:49.37 Air.in :fast @22:31:49.44 Air.seek :locked @22:31:50.45
Carousel.reconfigure :locked @22:31:50.62 Powering cartridge bus @22:31:53.28 Gate.setIR 1750,5000,0,6 @22:31:53.41 Gate.setIR 0,5000,0,6 @22:31:53.50 PuckHeater.configure :default @22:31:53.59 Gate.power :heat,:ON,limit to 5000mA @22:31:54.21 Mounted lysis Cartridge @22:31:54.27 WARNING: Using default puck temperature sensor calibration @22:31:54.29 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:31:55.90 SPR.command "read_sequences" @22:31:55.98 SPR.command "stats_on" @22:31:56.89 SPR.command "start 0 1" @22:31:57.60 SPR.start /var/log/esp/spr24sep2322h.spr.bz2 @22:31:59.64 Waiting for baseline SPR stabilization @22:31:59.66 Handoff.select :waste @22:31:59.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:32:06.48 Syringe tare is 52% of full scale at 22C (drifted 1%) @22:32:06.51 PinPort.in :locking @22:32:06.62 PinPort.seek :locked @22:32:07.15 Archive.in :fast @22:32:07.21 Lysis2.in :fast @22:32:07.30 Lysis1.seek 1100uL @22:32:07.44 Archive.seek :full @22:32:07.56 Lysis2.seek 1100uL @22:32:11.88 delayUntil "22:41:59.20" @22:41:59.22 Lysis2.in :DAmix @22:41:59.36 Lysis1.in :DAmix @22:41:59.90 Lysis2.seek 950uL @22:41:59.96 Lysis1.seek 950uL @22:42:20.91 delay 5 seconds @22:42:25.94 Handoff.select SPR @22:42:26.13 SPR.command "start 2 14" @22:42:26.72 SPR stabilized @22:42:26.91 delay 10 seconds @22:42:36.93 SPR.command "start 2 0" @22:42:40.09 Waiting up to 11:00 for SPR to enter state 1 @22:44:37.75 SPR.command "hold_on" @22:44:39.66 SPR Dilution 1 of 4 -- Experiment 2 with 450.0uL L1 + 450.0uL L2 @22:44:40.35 Lysis2.seek 500uL @22:44:40.40 Lysis1.seek 500uL @22:45:42.71 delay 15 seconds @22:45:57.73 SPR.command "hold_off" @22:46:00.61 Waiting up to 1:00:00 for SPR to enter state 7 @23:02:15.22 SPRsummary:-1.31RIU,115RIU,265RIU,1.25RIU after 1919s @23:16:32.26 delay 10 seconds @23:16:42.29 SPR.command "start 2 0" @23:16:44.98 Waiting up to 11:00 for SPR to enter state 1 @23:18:42.64 SPR.command "hold_on" @23:18:44.05 SPR Dilution 2 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @23:18:44.75 Lysis2.seek 275uL @23:18:44.79 Lysis1.seek 275uL @23:19:16.03 delay 15 seconds @23:19:31.06 SPR.command "hold_off" @23:19:34.28 Waiting up to 1:00:00 for SPR to enter state 7 @23:50:06.90 delay 10 seconds @23:50:16.92 SPR.command "start 2 0" @23:50:19.60 Waiting up to 11:00 for SPR to enter state 1 @23:52:17.26 SPR.command "hold_on" @23:52:18.65 SPR Dilution 3 of 4 -- Experiment 2 with 225.0uL L1 + 225.0uL L2 @23:52:19.36 Lysis2.seek 50uL @23:52:19.42 Lysis1.seek 50uL @23:52:50.64 delay 15 seconds @23:53:05.67 SPR.command "hold_off" @23:53:08.90 Waiting up to 1:00:00 for SPR to enter state 7 @00:23:41.46PDT24-Sep-24 Additional SPR Analyses Skipped due to Insufficient Volume @00:23:41.70 Handoff.select :close @00:23:41.89
Gate.power :heat,:OFF @00:23:41.91 Gate.power :cartridge,:OFF @00:23:42.07 PinPort.in :unlocking @00:23:42.15 PinPort.seek :clear @00:23:42.66 Carousel.in :default @00:23:42.80 Carousel.home.seek 49 @00:23:43.18 Plunge.seek :locked @00:23:43.45 Archive.in :unlocking @00:23:43.60 Archive.seek :clear @00:23:43.73 Air.in :unlocking @00:23:43.86 Air.seek :clear @00:23:44.05 Lysis1.in :unlocking @00:23:44.20 Lysis1.seek :clear @00:23:44.36 Lysis2.in :unlocking @00:23:44.45 Lysis2.seek :clear @00:24:05.19 Ended log spr24sep2322h.spr.bz2 @00:24:05.22
SPR.command "stats_off" @00:24:05.63 Gate.power :analytic1,:OFF @00:24:05.65 Gate.power :motor,:OFF @00:24:05.69 Gate.power :core,:OFF @00:24:05.72 Gate.power :main,:OFF @00:24:05.75 Safely stopped and ready to power off @00:24:05.94 Cartridge::None in MAIN -- Not enough standard cartridges remain @00:24:06.08 Session Ended