@17:44:10.22PDT21-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:44:10.25 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:44:14.78 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:44:15.49 Sea pressure (Abs) tare is 6% of full scale @17:44:19.04 Polling Can every 2:00 @17:44:19.09 Loading /home/methane/esp2/bin/processAll mission @17:44:19.37 SyntaxError in MAIN -- compile error --options:1: syntax error, unexpected ',', expecting ']' (c=Cartridge).options.clear; c.options extraDilutions:[1,1,,1] ^ @17:50:51.07PDT21-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:50:51.10 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:50:55.68 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:50:56.43 Sea pressure (Abs) tare is 6% of full scale @17:51:00.16 Polling Can every 2:00 @17:51:00.21 Loading /home/methane/esp2/bin/processAll mission @17:51:00.53 Sea pressure (Abs) tare is 6% of full scale @17:51:00.54 Sampling default goal volumes @17:51:00.56 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @17:51:00.72 Gate.power :analytic1,:ON,limit to 5000mA @17:51:00.73 Gate.power :analytic2,:ON,limit to 5000mA @17:51:00.75 Gate.power :analytic3,:ON,limit to 5000mA @17:51:01.50 Archive.configure :default @17:51:01.57 SPR Module 1, FW Rev. B, FPGA Rev. C @17:51:01.70
Air.configure :default @17:51:01.86 Lysis1.configure :default @17:51:01.99 Lysis2.configure :default @17:51:02.14 Plunge.configure :default @17:51:02.29 SamplePump.configure :default @17:51:02.45 Toroid.configure :default @17:51:02.51 Toroid.hold @17:51:02.58 Toroid.configure :default @17:51:02.73 Carousel.configure :default @17:51:02.79 Carousel.hold @17:51:02.86 Carousel.configure :default @17:51:03.01 Pressure.configure :default @17:51:03.17 ExtraPump.configure :default @17:51:03.29 PinPort.configure :default @17:51:03.35 All elf controllers running firmware version 4.83 @17:51:04.19 Toroid.seek :clear @17:51:04.26 Carousel.in :default @17:51:04.40 Carousel.home.seek 59 @17:51:04.65 Selecting Cartridge 56 @17:51:04.95 Carousel.seek 56 @17:51:06.47 Lysis1.in :fast @17:51:06.54 Lysis1.seek :locked @17:51:09.16 Plunge.seek :locked @17:51:09.76 Air.in :fast @17:51:09.83 Air.seek :locked @17:51:11.41
Carousel.reconfigure :locked @17:51:11.59 Powering cartridge bus @17:51:14.23 Gate.setIR 1750,5000,0,6 @17:51:14.36 Gate.setIR 0,5000,0,6 @17:51:14.43 PuckHeater.configure :default @17:51:14.48 Gate.power :heat,:ON,limit to 5000mA @17:51:15.27 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084 @17:51:15.31 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:51:16.87 SPR.command "read_sequences" @17:51:17.49 SPR.command "stats_on" @17:51:17.64 SPR.command "start 0 1" @17:51:18.35 SPR.start /var/log/methane/spr23mar2117h.spr.bz2 @17:51:20.84 Waiting for baseline SPR stabilization @17:51:20.86 Handoff.select :waste @17:51:27.56 Syringe tare is 16% of full scale @17:51:27.58 PinPort.in :locking @17:51:27.65 PinPort.seek :locked @17:51:28.14 Archive.in :fast @17:51:28.22 Lysis2.in :fast @17:51:28.25 Lysis1.seek 1000uL @17:51:28.44 Archive.seek :full @17:51:28.53 Lysis2.seek 1000uL @17:51:33.05 delayUntil "18:01:20.81" @18:01:20.82 Lysis2.in :DAmix @18:01:20.94 Lysis1.in :DAmix @18:01:21.48 Lysis2.seek 750uL @18:01:21.53 Lysis1.seek 750uL @18:01:56.68 delay 5 seconds @18:02:01.70 Handoff.select SPR @18:02:01.83 SPR.command "start 2 14" @18:02:03.06 SPR stabilized @18:02:03.20 delay 10 seconds @18:02:13.23 SPR.command "start 1 0" @18:02:15.93 Waiting up to 4:00 for SPR to enter state 1 @18:04:20.20 SPR.command "hold_on" @18:04:21.61 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:04:22.22 Lysis2.seek 450uL @18:04:22.27 Lysis1.seek 450uL @18:05:04.35 delay 15 seconds @18:05:19.37 SPR.command "hold_off" @18:05:22.26 Waiting up to 38:00 for SPR to enter state 7 @18:23:17.12 SPRsummary:8.44933,90.88,115.217,157.859 after 2001s @18:29:54.48 delay 10 seconds @18:30:04.51 SPR.command "start 1 0" @18:30:07.22 Waiting up to 4:00 for SPR to enter state 1 @18:32:11.49 SPR.command "hold_on" @18:32:12.89 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:32:13.50 Lysis2.seek 300uL @18:32:13.53 Lysis1.seek 300uL @18:32:34.64 delay 15 seconds @18:32:49.66 SPR.command "hold_off" @18:32:53.23 Waiting up to 38:00 for SPR to enter state 7 @18:57:26.38 delay 10 seconds @18:57:36.40 SPR.command "start 1 0" @18:57:40.09 Waiting up to 4:00 for SPR to enter state 1 @18:59:44.80 SPR.command "hold_on" @18:59:46.21 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:59:46.77 Lysis2.seek 150uL @18:59:46.81 Lysis1.seek 150uL @19:00:07.94 delay 15 seconds @19:00:22.96 SPR.command "hold_off" @19:00:26.05 Waiting up to 38:00 for SPR to enter state 7 @19:24:58.31 delay 10 seconds @19:25:08.33 SPR.command "start 1 0" @19:25:11.01 Waiting up to 4:00 for SPR to enter state 1 @19:27:15.29 SPR.command "hold_on" @19:27:16.69 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:27:17.25 Lysis2.seek 0uL @19:27:17.30 Lysis1.seek 0uL @19:27:38.42 delay 15 seconds @19:27:53.44 SPR.command "hold_off" @19:27:56.53 Waiting up to 38:00 for SPR to enter state 7 @19:52:28.64 SPR analysis completed @19:52:30.76 Ended log spr23mar2117h.spr.bz2 @19:52:30.78 SPR.command "stats_off" @19:52:31.54 Intake.close @19:52:31.58 Exhaust.close @19:52:31.72 Bypass.close @19:52:31.78 Handoff.select :close @19:52:31.99
Gate.power :heat,:OFF @19:52:32.00 Gate.power :cartridge,:OFF @19:52:32.09 PinPort.in :unlocking @19:52:32.15 PinPort.seek :clear @19:52:32.62 Carousel.in :default @19:52:32.74 Carousel.home.seek 56 @19:52:33.08 Plunge.seek :locked @19:52:33.24 Archive.in :unlocking @19:52:33.38 Archive.seek :clear @19:52:33.45 Air.in :unlocking @19:52:33.64 Air.seek :clear @19:52:33.77 Lysis1.in :unlocking @19:52:33.91 Lysis1.seek :clear @19:52:34.02 Lysis2.in :unlocking @19:52:34.09 Lysis2.seek :clear @19:52:39.19
Gate.power :motor,:OFF @19:52:39.21 Gate.power :core,:OFF @19:52:39.22 Gate.power :analytic1,:OFF @19:52:39.23 Gate.power :analytic2,:OFF @19:52:39.25 Gate.power :analytic3,:OFF @19:52:39.26 Gate.power :main,:OFF @19:52:39.27 Safely stopped and ready to power off @19:52:43.03 Gate.power :main,:ON,limit to 5000mA @19:52:43.05 Gate.power :analytic1,:ON,limit to 5000mA @19:52:43.06 Gate.power :analytic2,:ON,limit to 5000mA @19:52:43.07 Gate.power :analytic3,:ON,limit to 5000mA @19:52:43.17 Powering core bus @19:52:45.52 SPR Module 1, FW Rev. B, FPGA Rev. C @19:52:45.95
Archive.configure :default @19:52:46.05 Air.configure :default @19:52:46.11 Lysis1.configure :default @19:52:46.17 Lysis2.configure :default @19:52:46.26 Plunge.configure :default @19:52:46.35 SamplePump.configure :default @19:52:46.44 Toroid.configure :default @19:52:46.53 Carousel.configure :default @19:52:46.61 Pressure.configure :default @19:52:46.70 ExtraPump.configure :default @19:52:46.77 PinPort.configure :default @19:52:46.83 All elf controllers running firmware version 4.83 @19:52:46.85 Gate.power :motor,:ON,limit to 5000mA @19:52:47.24 Lysis1.home.jog 0 @19:52:47.32 Archive.home.jog 0 @19:52:47.50 Air.home.jog 0 @19:52:48.14 PinPort.home.jog 0 @19:52:48.19 Toroid.home.jog 0 @19:52:48.74 Plunge.home.jog 0 @19:52:48.77 Carousel.home.jog 0 @19:52:48.82 Lysis2.home.jog 0 @19:52:49.44 Air.in :homing @19:52:49.56 Archive.in :homing @19:52:49.64 Lysis1.in :homing @19:52:49.74 Air.jog 1379 @19:52:49.84 Archive.jog 1372 @19:52:49.89 Lysis1.jog 1383 @19:52:50.15 PinPort.in :homing @19:52:50.20 Toroid.in :homing @19:52:50.23 Plunge.in :homing @19:52:50.26 Lysis2.in :homing @19:52:50.41 PinPort.jog -107 @19:52:50.48 Plunge.jog 1520 @19:52:50.55 Toroid.jog 617 @19:52:50.60 Lysis2.jog 1391 @19:52:50.83 PinPort.setRawPosition! 0 @19:52:50.87 PinPort.seek :home @19:52:50.96 PinPort.in :default @19:52:51.05 Air.setRawPosition! 50 @19:52:51.13 Air.seek :home @19:52:51.22 Lysis1.setRawPosition! 50 @19:52:51.25 Archive.setRawPosition! 50 @19:52:51.32 Lysis1.seek :home @19:52:51.37 Archive.seek :home @19:52:51.53 Air.in :default @19:52:51.68 Lysis1.in :default @19:52:51.77 Archive.in :default @19:52:51.92 Plunge.setRawPosition! 50 @19:52:51.94 Lysis2.setRawPosition! 50 @19:52:52.04 Plunge.seek :home @19:52:52.07 Lysis2.seek :home @19:52:52.39 Plunge.in :default @19:52:52.47 Lysis2.in :default @19:52:52.76 Toroid.setRawPosition! 0 @19:52:52.82 Toroid.seek :home @19:52:52.90 Toroid.in :default @19:52:52.98
Toroid.seek :clear @19:52:54.59 Carousel.in :default @19:52:54.72 Carousel.home.seek 56 @19:52:54.97 Selecting Cartridge 55 @19:52:55.26 Carousel.seek 55 @19:52:56.35 Lysis1.in :fast @19:52:56.41 Lysis1.seek :locked @19:52:59.03 Plunge.seek :locked @19:52:59.18 Air.in :fast @19:52:59.24 Air.seek :locked @19:53:01.30
Carousel.reconfigure :locked @19:53:01.45 Powering cartridge bus @19:53:08.09 Gate.power :cartridge,:OFF @19:53:08.13 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @19:53:08.14 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @10:46:23.15PDT22-Mar-23 Session Begun @10:46:25.61 -> showlog @19:53:08.09 Gate.power :cartridge,:OFF @19:53:08.13 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @19:53:08.14 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @10:46:23.15PDT22-Mar-23 Session Begun @10:46:25.61 -> showlog @10:46:48.36 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23mar2117h.spr.bz2}} @10:47:06.01 -> showlog -200 @17:51:02.79 Carousel.hold @17:51:02.86 Carousel.configure :default @17:51:03.01 Pressure.configure :default @17:51:03.17 ExtraPump.configure :default @17:51:03.29 PinPort.configure :default @17:51:03.35 All elf controllers running firmware version 4.83 @17:51:04.19 Toroid.seek :clear @17:51:04.26 Carousel.in :default @17:51:04.40 Carousel.home.seek 59 @17:51:04.65 Selecting Cartridge 56 @17:51:04.95 Carousel.seek 56 @17:51:06.47 Lysis1.in :fast @17:51:06.54 Lysis1.seek :locked @17:51:09.16 Plunge.seek :locked @17:51:09.76 Air.in :fast @17:51:09.83 Air.seek :locked @17:51:11.41
Carousel.reconfigure :locked @17:51:11.59 Powering cartridge bus @17:51:14.23 Gate.setIR 1750,5000,0,6 @17:51:14.36 Gate.setIR 0,5000,0,6 @17:51:14.43 PuckHeater.configure :default @17:51:14.48 Gate.power :heat,:ON,limit to 5000mA @17:51:15.27 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084 @17:51:15.31 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:51:16.87 SPR.command "read_sequences" @17:51:17.49 SPR.command "stats_on" @17:51:17.64 SPR.command "start 0 1" @17:51:18.35 SPR.start /var/log/methane/spr23mar2117h.spr.bz2 @17:51:20.84 Waiting for baseline SPR stabilization @17:51:20.86 Handoff.select :waste @17:51:27.56 Syringe tare is 16% of full scale @17:51:27.58 PinPort.in :locking @17:51:27.65 PinPort.seek :locked @17:51:28.14 Archive.in :fast @17:51:28.22 Lysis2.in :fast @17:51:28.25 Lysis1.seek 1000uL @17:51:28.44 Archive.seek :full @17:51:28.53 Lysis2.seek 1000uL @17:51:33.05 delayUntil "18:01:20.81" @18:01:20.82 Lysis2.in :DAmix @18:01:20.94 Lysis1.in :DAmix @18:01:21.48 Lysis2.seek 750uL @18:01:21.53 Lysis1.seek 750uL @18:01:56.68 delay 5 seconds @18:02:01.70 Handoff.select SPR @18:02:01.83 SPR.command "start 2 14" @18:02:03.06 SPR stabilized @18:02:03.20 delay 10 seconds @18:02:13.23 SPR.command "start 1 0" @18:02:15.93 Waiting up to 4:00 for SPR to enter state 1 @18:04:20.20 SPR.command "hold_on" @18:04:21.61 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:04:22.22 Lysis2.seek 450uL @18:04:22.27 Lysis1.seek 450uL @18:05:04.35 delay 15 seconds @18:05:19.37 SPR.command "hold_off" @18:05:22.26 Waiting up to 38:00 for SPR to enter state 7 @18:23:17.12 SPRsummary:8.44933,90.88,115.217,157.859 after 2001s @18:29:54.48 delay 10 seconds @18:30:04.51 SPR.command "start 1 0" @18:30:07.22 Waiting up to 4:00 for SPR to enter state 1 @18:32:11.49 SPR.command "hold_on" @18:32:12.89 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:32:13.50 Lysis2.seek 300uL @18:32:13.53 Lysis1.seek 300uL @18:32:34.64 delay 15 seconds @18:32:49.66 SPR.command "hold_off" @18:32:53.23 Waiting up to 38:00 for SPR to enter state 7 @18:57:26.38 delay 10 seconds @18:57:36.40 SPR.command "start 1 0" @18:57:40.09 Waiting up to 4:00 for SPR to enter state 1 @18:59:44.80 SPR.command "hold_on" @18:59:46.21 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:59:46.77 Lysis2.seek 150uL @18:59:46.81 Lysis1.seek 150uL @19:00:07.94 delay 15 seconds @19:00:22.96 SPR.command "hold_off" @19:00:26.05 Waiting up to 38:00 for SPR to enter state 7 @19:24:58.31 delay 10 seconds @19:25:08.33 SPR.command "start 1 0" @19:25:11.01 Waiting up to 4:00 for SPR to enter state 1 @19:27:15.29 SPR.command "hold_on" @19:27:16.69 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:27:17.25 Lysis2.seek 0uL @19:27:17.30 Lysis1.seek 0uL @19:27:38.42 delay 15 seconds @19:27:53.44 SPR.command "hold_off" @19:27:56.53 Waiting up to 38:00 for SPR to enter state 7 @19:52:28.64 SPR analysis completed @19:52:30.76 Ended log spr23mar2117h.spr.bz2 @19:52:30.78 SPR.command "stats_off" @19:52:31.54 Intake.close @19:52:31.58 Exhaust.close @19:52:31.72 Bypass.close @19:52:31.78 Handoff.select :close @19:52:31.99
Gate.power :heat,:OFF @19:52:32.00 Gate.power :cartridge,:OFF @19:52:32.09 PinPort.in :unlocking @19:52:32.15 PinPort.seek :clear @19:52:32.62 Carousel.in :default @19:52:32.74 Carousel.home.seek 56 @19:52:33.08 Plunge.seek :locked @19:52:33.24 Archive.in :unlocking @19:52:33.38 Archive.seek :clear @19:52:33.45 Air.in :unlocking @19:52:33.64 Air.seek :clear @19:52:33.77 Lysis1.in :unlocking @19:52:33.91 Lysis1.seek :clear @19:52:34.02 Lysis2.in :unlocking @19:52:34.09 Lysis2.seek :clear @19:52:39.19
Gate.power :motor,:OFF @19:52:39.21 Gate.power :core,:OFF @19:52:39.22 Gate.power :analytic1,:OFF @19:52:39.23 Gate.power :analytic2,:OFF @19:52:39.25 Gate.power :analytic3,:OFF @19:52:39.26 Gate.power :main,:OFF @19:52:39.27 Safely stopped and ready to power off @19:52:43.03 Gate.power :main,:ON,limit to 5000mA @19:52:43.05 Gate.power :analytic1,:ON,limit to 5000mA @19:52:43.06 Gate.power :analytic2,:ON,limit to 5000mA @19:52:43.07 Gate.power :analytic3,:ON,limit to 5000mA @19:52:43.17 Powering core bus @19:52:45.52 SPR Module 1, FW Rev. B, FPGA Rev. C @19:52:45.95
Archive.configure :default @19:52:46.05 Air.configure :default @19:52:46.11 Lysis1.configure :default @19:52:46.17 Lysis2.configure :default @19:52:46.26 Plunge.configure :default @19:52:46.35 SamplePump.configure :default @19:52:46.44 Toroid.configure :default @19:52:46.53 Carousel.configure :default @19:52:46.61 Pressure.configure :default @19:52:46.70 ExtraPump.configure :default @19:52:46.77 PinPort.configure :default @19:52:46.83 All elf controllers running firmware version 4.83 @19:52:46.85 Gate.power :motor,:ON,limit to 5000mA @19:52:47.24 Lysis1.home.jog 0 @19:52:47.32 Archive.home.jog 0 @19:52:47.50 Air.home.jog 0 @19:52:48.14 PinPort.home.jog 0 @19:52:48.19 Toroid.home.jog 0 @19:52:48.74 Plunge.home.jog 0 @19:52:48.77 Carousel.home.jog 0 @19:52:48.82 Lysis2.home.jog 0 @19:52:49.44 Air.in :homing @19:52:49.56 Archive.in :homing @19:52:49.64 Lysis1.in :homing @19:52:49.74 Air.jog 1379 @19:52:49.84 Archive.jog 1372 @19:52:49.89 Lysis1.jog 1383 @19:52:50.15 PinPort.in :homing @19:52:50.20 Toroid.in :homing @19:52:50.23 Plunge.in :homing @19:52:50.26 Lysis2.in :homing @19:52:50.41 PinPort.jog -107 @19:52:50.48 Plunge.jog 1520 @19:52:50.55 Toroid.jog 617 @19:52:50.60 Lysis2.jog 1391 @19:52:50.83 PinPort.setRawPosition! 0 @19:52:50.87 PinPort.seek :home @19:52:50.96 PinPort.in :default @19:52:51.05 Air.setRawPosition! 50 @19:52:51.13 Air.seek :home @19:52:51.22 Lysis1.setRawPosition! 50 @19:52:51.25 Archive.setRawPosition! 50 @19:52:51.32 Lysis1.seek :home @19:52:51.37 Archive.seek :home @19:52:51.53 Air.in :default @19:52:51.68 Lysis1.in :default @19:52:51.77 Archive.in :default @19:52:51.92 Plunge.setRawPosition! 50 @19:52:51.94 Lysis2.setRawPosition! 50 @19:52:52.04 Plunge.seek :home @19:52:52.07 Lysis2.seek :home @19:52:52.39 Plunge.in :default @19:52:52.47 Lysis2.in :default @19:52:52.76 Toroid.setRawPosition! 0 @19:52:52.82 Toroid.seek :home @19:52:52.90 Toroid.in :default @19:52:52.98
Toroid.seek :clear @19:52:54.59 Carousel.in :default @19:52:54.72 Carousel.home.seek 56 @19:52:54.97 Selecting Cartridge 55 @19:52:55.26 Carousel.seek 55 @19:52:56.35 Lysis1.in :fast @19:52:56.41 Lysis1.seek :locked @19:52:59.03 Plunge.seek :locked @19:52:59.18 Air.in :fast @19:52:59.24 Air.seek :locked @19:53:01.30
Carousel.reconfigure :locked @19:53:01.45 Powering cartridge bus @19:53:08.09 Gate.power :cartridge,:OFF @19:53:08.13 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @19:53:08.14 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @10:46:23.15PDT22-Mar-23 Session Begun @10:46:25.61 -> showlog @19:53:08.09 Gate.power :cartridge,:OFF @19:53:08.13 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @19:53:08.14 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @10:46:23.15PDT22-Mar-23 Session Begun @10:46:25.61 -> showlog @10:46:48.36 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23mar2117h.spr.bz2}} @10:47:06.01 -> showlog -200 @12:59:04.98 Session Ended @13:14:03.73 Session Begun @13:14:11.10 -> Cmd.resume @13:14:11.13 Cmd::Error in bill -- Cannot resume from IDLE state @13:14:23.86 -> Cartridge.unlock @13:14:24.55 Carousel.in :default @13:14:24.71 Carousel.home.seek 55 @13:14:25.19 Air.in :unlocking @13:14:25.34 Air.seek :clear @13:14:26.71 Lysis1.in :unlocking @13:14:26.90 Lysis1.seek :clear @13:14:27.00 Plunge.in :unlocking @13:14:27.12 Plunge.seek :clear @13:14:34.93 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr23mar2117h.spr.bz2}} @13:14:41.72 -> exit @13:14:41.74 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/methane/real.log @13:16:15.13 Session Begun @13:16:20.96 -> ESP.ready! @13:16:21.75 Toroid.seek :clear @13:16:21.89 Carousel.in :default @13:16:22.04 Carousel.home.seek 55 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Pressure = Absolute Pressure (Leak) Sensor @17psia PinPort = Handoff Pin Port (PP) clear @13:16:33.40 -> ESP.reready! @13:16:33.46 Archive.in :default @13:16:33.50 Air.in :default @13:16:33.63 Lysis1.in :default @13:16:33.75 Lysis2.in :default @13:16:33.99 Archive.home.jog 0 @13:16:34.02 Plunge.in :default @13:16:34.09 Toroid.in :default @13:16:34.17 Carousel.in :default @13:16:34.28 Air.home.jog 0 @13:16:34.33 PinPort.in :default @13:16:34.47 Lysis1.home.jog 0 @13:16:34.62 Lysis2.home.jog 0 @13:16:34.77 Plunge.home.jog 0 @13:16:34.86 Toroid.home.jog 0 @13:16:34.91 Carousel.home.jog 0 @13:16:35.00 PinPort.home.jog 0 @13:16:35.73 Archive.in :homing @13:16:36.20 Archive.jog 1370 @13:16:36.29 PinPort.in :homing @13:16:36.34 Plunge.in :homing @13:16:36.40 Lysis1.in :homing @13:16:36.47 Toroid.in :homing @13:16:36.50 Air.in :homing @13:16:36.55 Lysis2.in :homing @13:16:37.44 PinPort.jog -107 @13:16:37.48 Plunge.jog 378 @13:16:37.54 Lysis1.jog 1385 @13:16:37.57 Air.jog 1379 @13:16:37.63 Toroid.jog 625 @13:16:37.69 Lysis2.jog 1387 @13:16:38.20 PinPort.setRawPosition! 0 @13:16:38.23 Archive.setRawPosition! 50 @13:16:38.36 PinPort.seek :home @13:16:38.41 Archive.seek :home @13:16:38.67 Plunge.setRawPosition! 50 @13:16:38.71 PinPort.in :default @13:16:38.85 Plunge.seek :home @13:16:38.99 Archive.in :default @13:16:39.12 Lysis1.setRawPosition! 50 @13:16:39.21 Lysis1.seek :home @13:16:39.36 Plunge.in :default @13:16:39.40 Lysis2.setRawPosition! 50 @13:16:39.44 Air.setRawPosition! 50 @13:16:39.65 Lysis2.seek :home @13:16:39.71 Air.seek :home @13:16:39.97 Lysis1.in :default @13:16:40.15 Air.in :default @13:16:40.18 Lysis2.in :default @13:16:40.37 Toroid.setRawPosition! 0 @13:16:40.44 Toroid.seek :home @13:16:40.57 Toroid.in :default @13:16:40.70 Toroid.seek :clear @13:16:42.34 Carousel.in :default @13:16:42.50 Carousel.home.seek 55 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 55 Pressure = Absolute Pressure (Leak) Sensor @17psia PinPort = Handoff Pin Port (PP) clear @13:16:47.72 -> exit @13:16:47.75 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/methane/real.log @13:19:07.52 Session Begun @13:19:13.60 -> MainThread.exit # @13:19:13.88 ThreadError in bill -- killed thread @13:19:13.94 Session Ended @13:20:31.23PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:20:31.26 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:20:35.74 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:20:36.49 Sea pressure (Abs) tare is 6% of full scale @13:20:40.18 Polling Can every 2:00 @13:20:40.23 Loading /home/methane/esp2/bin/processAll mission @13:20:40.51 SyntaxError in MAIN -- compile error --options:1: syntax error, unexpected $end (c=Cartridge).options.clear; c.options extraDilutions: ^ @13:25:38.31PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:25:38.35 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:25:42.93 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:25:43.66 Sea pressure (Abs) tare is 6% of full scale @13:25:47.34 Polling Can every 2:00 @13:25:47.38 Loading /home/methane/esp2/bin/processAll mission @13:25:47.70 Sea pressure (Abs) tare is 6% of full scale @13:25:47.72 Sampling default goal volumes @13:25:47.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:25:47.90 Gate.power :analytic1,:ON,limit to 5000mA @13:25:47.92 Gate.power :analytic2,:ON,limit to 5000mA @13:25:47.93 Gate.power :analytic3,:ON,limit to 5000mA @13:25:48.70 Archive.configure :default @13:25:48.80 SPR Module 1, FW Rev. B, FPGA Rev. C @13:25:48.94
Air.configure :default @13:25:49.07 Lysis1.configure :default @13:25:49.21 Lysis2.configure :default @13:25:49.38 Plunge.configure :default @13:25:49.66 SamplePump.configure :default @13:25:49.84 Toroid.configure :default @13:25:49.91 Toroid.hold @13:25:49.98 Toroid.configure :default @13:25:50.15 Carousel.configure :default @13:25:50.21 Carousel.hold @13:25:50.28 Carousel.configure :default @13:25:50.44 Pressure.configure :default @13:25:50.59 ExtraPump.configure :default @13:25:50.74 PinPort.configure :default @13:25:50.81 All elf controllers running firmware version 4.83 @13:25:51.57 Toroid.seek :clear @13:25:51.65 Carousel.in :default @13:25:51.79 Carousel.home.seek 55 @13:25:52.05 Selecting Cartridge 55 @13:25:52.34 Carousel.seek 55 @13:25:53.15 Lysis1.in :fast @13:25:53.21 Lysis1.seek :locked @13:25:55.85 Plunge.seek :locked @13:25:56.48 Air.in :fast @13:25:56.55 Air.seek :locked @13:25:58.12
Carousel.reconfigure :locked @13:25:58.30 Powering cartridge bus @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:36:38.20 -> showlog -300 @13:25:38.31PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:25:38.35 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:25:42.93 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:25:43.66 Sea pressure (Abs) tare is 6% of full scale @13:25:47.34 Polling Can every 2:00 @13:25:47.38 Loading /home/methane/esp2/bin/processAll mission @13:25:47.70 Sea pressure (Abs) tare is 6% of full scale @13:25:47.72 Sampling default goal volumes @13:25:47.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:25:47.90 Gate.power :analytic1,:ON,limit to 5000mA @13:25:47.92 Gate.power :analytic2,:ON,limit to 5000mA @13:25:47.93 Gate.power :analytic3,:ON,limit to 5000mA @13:25:48.70 Archive.configure :default @13:25:48.80 SPR Module 1, FW Rev. B, FPGA Rev. C @13:25:48.94
Air.configure :default @13:25:49.07 Lysis1.configure :default @13:25:49.21 Lysis2.configure :default @13:25:49.38 Plunge.configure :default @13:25:49.66 SamplePump.configure :default @13:25:49.84 Toroid.configure :default @13:25:49.91 Toroid.hold @13:25:49.98 Toroid.configure :default @13:25:50.15 Carousel.configure :default @13:25:50.21 Carousel.hold @13:25:50.28 Carousel.configure :default @13:25:50.44 Pressure.configure :default @13:25:50.59 ExtraPump.configure :default @13:25:50.74 PinPort.configure :default @13:25:50.81 All elf controllers running firmware version 4.83 @13:25:51.57 Toroid.seek :clear @13:25:51.65 Carousel.in :default @13:25:51.79 Carousel.home.seek 55 @13:25:52.05 Selecting Cartridge 55 @13:25:52.34 Carousel.seek 55 @13:25:53.15 Lysis1.in :fast @13:25:53.21 Lysis1.seek :locked @13:25:55.85 Plunge.seek :locked @13:25:56.48 Air.in :fast @13:25:56.55 Air.seek :locked @13:25:58.12
Carousel.reconfigure :locked @13:25:58.30 Powering cartridge bus @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:36:38.20 -> showlog -300 @13:40:06.38 -> showlog -500 @13:25:38.31PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:25:38.35 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:25:42.93 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:25:43.66 Sea pressure (Abs) tare is 6% of full scale @13:25:47.34 Polling Can every 2:00 @13:25:47.38 Loading /home/methane/esp2/bin/processAll mission @13:25:47.70 Sea pressure (Abs) tare is 6% of full scale @13:25:47.72 Sampling default goal volumes @13:25:47.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:25:47.90 Gate.power :analytic1,:ON,limit to 5000mA @13:25:47.92 Gate.power :analytic2,:ON,limit to 5000mA @13:25:47.93 Gate.power :analytic3,:ON,limit to 5000mA @13:25:48.70 Archive.configure :default @13:25:48.80 SPR Module 1, FW Rev. B, FPGA Rev. C @13:25:48.94
Air.configure :default @13:25:49.07 Lysis1.configure :default @13:25:49.21 Lysis2.configure :default @13:25:49.38 Plunge.configure :default @13:25:49.66 SamplePump.configure :default @13:25:49.84 Toroid.configure :default @13:25:49.91 Toroid.hold @13:25:49.98 Toroid.configure :default @13:25:50.15 Carousel.configure :default @13:25:50.21 Carousel.hold @13:25:50.28 Carousel.configure :default @13:25:50.44 Pressure.configure :default @13:25:50.59 ExtraPump.configure :default @13:25:50.74 PinPort.configure :default @13:25:50.81 All elf controllers running firmware version 4.83 @13:25:51.57 Toroid.seek :clear @13:25:51.65 Carousel.in :default @13:25:51.79 Carousel.home.seek 55 @13:25:52.05 Selecting Cartridge 55 @13:25:52.34 Carousel.seek 55 @13:25:53.15 Lysis1.in :fast @13:25:53.21 Lysis1.seek :locked @13:25:55.85 Plunge.seek :locked @13:25:56.48 Air.in :fast @13:25:56.55 Air.seek :locked @13:25:58.12
Carousel.reconfigure :locked @13:25:58.30 Powering cartridge bus @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:36:38.20 -> showlog -300 @13:25:38.31PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:25:38.35 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:25:42.93 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:25:43.66 Sea pressure (Abs) tare is 6% of full scale @13:25:47.34 Polling Can every 2:00 @13:25:47.38 Loading /home/methane/esp2/bin/processAll mission @13:25:47.70 Sea pressure (Abs) tare is 6% of full scale @13:25:47.72 Sampling default goal volumes @13:25:47.74 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:25:47.90 Gate.power :analytic1,:ON,limit to 5000mA @13:25:47.92 Gate.power :analytic2,:ON,limit to 5000mA @13:25:47.93 Gate.power :analytic3,:ON,limit to 5000mA @13:25:48.70 Archive.configure :default @13:25:48.80 SPR Module 1, FW Rev. B, FPGA Rev. C @13:25:48.94
Air.configure :default @13:25:49.07 Lysis1.configure :default @13:25:49.21 Lysis2.configure :default @13:25:49.38 Plunge.configure :default @13:25:49.66 SamplePump.configure :default @13:25:49.84 Toroid.configure :default @13:25:49.91 Toroid.hold @13:25:49.98 Toroid.configure :default @13:25:50.15 Carousel.configure :default @13:25:50.21 Carousel.hold @13:25:50.28 Carousel.configure :default @13:25:50.44 Pressure.configure :default @13:25:50.59 ExtraPump.configure :default @13:25:50.74 PinPort.configure :default @13:25:50.81 All elf controllers running firmware version 4.83 @13:25:51.57 Toroid.seek :clear @13:25:51.65 Carousel.in :default @13:25:51.79 Carousel.home.seek 55 @13:25:52.05 Selecting Cartridge 55 @13:25:52.34 Carousel.seek 55 @13:25:53.15 Lysis1.in :fast @13:25:53.21 Lysis1.seek :locked @13:25:55.85 Plunge.seek :locked @13:25:56.48 Air.in :fast @13:25:56.55 Air.seek :locked @13:25:58.12
Carousel.reconfigure :locked @13:25:58.30 Powering cartridge bus @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:26:04.94 Gate.power :cartridge,:OFF @13:26:04.97 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @13:26:04.98 Waiting to Resume from Checkpoint "Selecting Cartridge 55" @13:26:42.35 Session Begun @13:26:44.32 -> showlog @13:36:38.20 -> showlog -300 @13:40:06.38 -> showlog -500 @13:41:09.54 -> Cmd.loadCartridge @13:41:09.57 Cartridge::None in bill -- No dry cartridges remain @13:41:17.64 -> Cmd.loadCartridge 55 @13:41:18.47 Toroid.seek :clear @13:41:18.58 Carousel.in :default @13:41:18.78 Carousel.home.seek 55 @13:41:19.24 Carousel.reconfigure :locked @13:41:19.43 Powering cartridge bus @13:41:26.07 Gate.power :cartridge,:OFF @13:41:26.11 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:41:26.14 Retry #1 of 2 @13:41:26.29 Intake.close @13:41:26.36 Exhaust.close @13:41:26.43 Bypass.close @13:41:27.44 Gate.power :motor,:OFF @13:41:27.48 Gate.power :core,:OFF @13:41:27.52 Gate.power :analytic1,:OFF @13:41:27.57 Gate.power :analytic2,:OFF @13:41:27.61 Gate.power :analytic3,:OFF @13:41:27.65 Gate.power :main,:OFF @13:41:31.32 Gate.power :main,:ON,limit to 5000mA @13:41:31.36 Gate.power :analytic1,:ON,limit to 5000mA @13:41:31.40 Gate.power :analytic2,:ON,limit to 5000mA @13:41:31.44 Gate.power :analytic3,:ON,limit to 5000mA @13:41:31.59 Powering core bus @13:41:33.90 SPR Module 1, FW Rev. B, FPGA Rev. C @13:41:34.36 Archive.configure :default @13:41:34.49 Air.configure :default @13:41:34.59 Lysis1.configure :default @13:41:34.73 Lysis2.configure :default @13:41:34.85 Plunge.configure :default @13:41:34.98 SamplePump.configure :default @13:41:35.11 Toroid.configure :default @13:41:35.24 Carousel.configure :locked @13:41:35.36 Pressure.configure :default @13:41:35.48 ExtraPump.configure :default @13:41:35.58 PinPort.configure :default @13:41:35.68 All elf controllers running firmware version 4.83 @13:41:35.72 Gate.power :motor,:ON,limit to 5000mA @13:41:36.20 Archive.home.jog 0 @13:41:36.88 Carousel.in :default @13:41:36.94 Toroid.home.jog 0 @13:41:37.00 Plunge.home.jog 0 @13:41:37.07 Lysis1.home.jog 0 @13:41:37.11 Air.home.jog 0 @13:41:37.18 PinPort.home.jog 0 @13:41:37.22 Lysis2.home.jog 0 @13:41:38.15 Carousel.home.jog 0 @13:41:38.77 Archive.in :homing @13:41:39.14 Archive.jog 1373 @13:41:39.23 Plunge.in :homing @13:41:39.29 PinPort.in :homing @13:41:39.34 Lysis1.in :homing @13:41:39.42 Toroid.in :homing @13:41:39.48 Air.in :homing @13:41:39.56 Lysis2.in :homing @13:41:39.96 Plunge.jog 1531 @13:41:40.03 PinPort.jog -107 @13:41:40.07 Toroid.jog 625 @13:41:40.13 Lysis1.jog 2134 @13:41:40.19 Air.jog 1982 @13:41:40.29 Lysis2.jog 1387 @13:41:40.75 PinPort.setRawPosition! 0 @13:41:40.84 PinPort.seek :home @13:41:41.01 Archive.setRawPosition! 50 @13:41:41.04 PinPort.in :default @13:41:41.19 Archive.seek :home @13:41:41.53 Plunge.setRawPosition! 50 @13:41:41.61 Plunge.seek :home @13:41:41.76 Archive.in :default @13:41:41.79 Lysis2.setRawPosition! 50 @13:41:41.98 Lysis2.seek :home @13:41:42.11 Plunge.in :default @13:41:42.17 Lysis1.setRawPosition! 50 @13:41:42.22 Air.setRawPosition! 50 @13:41:42.40 Lysis1.seek :home @13:41:42.56 Air.seek :home @13:41:42.77 Lysis2.in :default @13:41:42.81 Toroid.setRawPosition! 0 @13:41:43.03 Toroid.seek :home @13:41:43.19 Lysis1.in :default @13:41:43.24 Air.in :default @13:41:43.30 Toroid.in :default @13:41:43.76 Toroid.seek :clear @13:41:45.41 Carousel.in :default @13:41:45.58 Carousel.home.seek 55 @13:41:45.91 Cartridge::Error in bill -- Cannot load standard cartridge 55 @13:41:57.88 -> Power {analytic1: true, analytic2: true, analytic3: true, cartridge: false, core: true, heat: false, main: true, motor: true} @13:42:13.52 -> Cart.lock @13:42:14.24 Lysis1.in :fast @13:42:14.33 Lysis1.seek :locked @13:42:16.98 Plunge.seek :locked @13:42:17.17 Air.in :fast @13:42:17.27 Air.seek :locked @13:42:18.89 Carousel.reconfigure :locked @13:42:19.09 Powering cartridge bus @13:42:25.73 Gate.power :cartridge,:OFF @13:42:25.77 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:42:45.07 -> to 56 @13:42:46.30 Carousel.in :default @13:42:46.46 Carousel.home.seek 55 @13:42:46.93 Air.in :unlocking @13:42:47.10 Air.seek :clear @13:42:48.53 Lysis1.in :unlocking @13:42:48.74 Lysis1.seek :clear @13:42:48.80 Plunge.in :unlocking @13:42:48.96 Plunge.seek :clear @13:42:50.20 Carousel.seek 56 @13:42:51.33 Lysis1.in :fast @13:42:51.43 Lysis1.seek :locked @13:42:54.08 Plunge.in :default @13:42:54.21 Plunge.seek :locked @13:42:54.35 Air.in :fast @13:42:54.44 Air.seek :locked @13:42:56.04 Carousel.reconfigure :locked @13:42:56.23 Powering cartridge bus @13:43:02.88 Gate.power :cartridge,:OFF @13:43:02.91 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:43:50.27 -> Cart.activate @13:43:50.42 Powering cartridge bus @13:43:57.07 Gate.power :cartridge,:OFF @13:43:57.10 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:44:19.91 -> Cart.activate @13:44:20.06 Powering cartridge bus @13:44:26.73 Gate.power :cartridge,:OFF @13:44:26.76 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:45:02.50 -> Cart.unlock @13:45:03.20 Carousel.in :default @13:45:03.35 Carousel.home.seek 56 @13:45:03.81 Air.in :unlocking @13:45:04.02 Air.seek :clear @13:45:05.44 Lysis1.in :unlocking @13:45:05.61 Lysis1.seek :clear @13:45:05.67 Plunge.in :unlocking @13:45:06.23 Plunge.seek :clear @13:45:40.66 -> Cart.activate @13:45:40.82 Powering cartridge bus @13:45:47.47 Gate.power :cartridge,:OFF @13:45:47.51 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:47:16.85 -> Power {analytic1: true, analytic2: true, analytic3: true, cartridge: false, core: true, heat: false, main: true, motor: true} @13:47:24.90 -> Cart.activate @13:47:25.04 Powering cartridge bus @13:47:27.69 Gate.setIR 1750,5000,0,6 @13:47:27.82 Gate.setIR 0,5000,0,6 @13:47:27.92 PuckHeater.configure :default @13:47:28.00 Gate.power :heat,:ON,limit to 5000mA @13:47:28.83 Mounted lysis Cartridge board#Q05054 @13:47:28.88 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @13:47:46.42 -> Cart.deactivate @13:47:46.43 Gate.power :heat,:OFF @13:47:46.47 Gate.power :cartridge,:OFF false @13:48:57.10 -> Cart.activate @13:48:57.25 Powering cartridge bus @13:49:03.89 Gate.power :cartridge,:OFF @13:49:03.92 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:49:08.93 -> Power {analytic1: true, analytic2: true, analytic3: true, cartridge: false, core: true, heat: false, main: true, motor: true} @13:49:30.73 -> Cart.activate @13:49:30.88 Powering cartridge bus @13:49:33.52 Gate.setIR 1750,5000,0,6 @13:49:33.66 Gate.setIR 0,5000,0,6 @13:49:33.74 PuckHeater.configure :default @13:49:33.82 Gate.power :heat,:ON,limit to 5000mA @13:49:34.58 Mounted lysis Cartridge board#Q05054 @13:49:34.67 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @13:49:39.53 -> Cart.deactivate @13:49:39.55 Gate.power :heat,:OFF @13:49:39.59 Gate.power :cartridge,:OFF false @13:50:34.68 -> Cart.activate @13:50:34.83 Powering cartridge bus @13:50:37.48 Gate.setIR 1750,5000,0,6 @13:50:37.61 Gate.setIR 0,5000,0,6 @13:50:37.70 PuckHeater.configure :default @13:50:37.79 Gate.power :heat,:ON,limit to 5000mA @13:50:38.51 Mounted lysis Cartridge board#Q05054 @13:50:38.56 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @13:50:46.67 -> Cart.deactivate @13:50:46.69 Gate.power :heat,:OFF @13:50:46.72 Gate.power :cartridge,:OFF false @13:53:03.98 -> Cart.activate @13:53:04.12 Powering cartridge bus @13:53:10.76 Gate.power :cartridge,:OFF @13:53:10.80 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:53:46.99 -> Cart.deactivate @13:54:09.29 -> Cart.activate @13:54:09.43 Powering cartridge bus @13:54:16.07 Gate.power :cartridge,:OFF @13:54:16.10 I2C::SoftErr in bill -- Bus Error writing to 0x00 @13:55:05.47 -> Cart.activate @13:55:05.62 Powering cartridge bus @13:55:08.28 Gate.setIR 1750,5000,0,6 @13:55:08.41 Gate.setIR 0,5000,0,6 @13:55:08.50 PuckHeater.configure :default @13:55:08.58 Gate.power :heat,:ON,limit to 5000mA @13:55:09.37 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:55:09.43 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @14:04:45.26 -> Cartridge.activate @14:04:45.47 Gate.setIR 1750,5000,0,6 @14:04:45.60 Gate.setIR 0,5000,0,6 @14:04:45.69 PuckHeater.configure :default @14:04:45.77 Gate.power :heat,:ON,limit to 5000mA @14:04:46.53 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084 @14:04:46.60 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @14:04:54.14 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:08:05.41 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:08:07.70 -> Cartridge { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"} @14:08:24.81 -> Cartridge.load @14:08:24.84 NoMethodError in bill -- undefined method `load' for Cartridge @14:08:31.80 -> Cartridge.lock @14:08:31.82 Gate.power :heat,:OFF @14:08:31.86 Gate.power :cartridge,:OFF @14:08:32.06 Lysis1.in :fast @14:08:32.15 Lysis1.seek :locked @14:08:35.27 Plunge.in :default @14:08:35.43 Plunge.seek :locked @14:08:35.55 Air.in :fast @14:08:35.65 Air.seek :locked @14:08:37.25 Carousel.reconfigure :locked @14:08:37.45 Powering cartridge bus @14:08:40.11 Gate.setIR 1750,5000,0,6 @14:08:40.24 Gate.setIR 0,5000,0,6 @14:08:40.33 PuckHeater.configure :default @14:08:40.42 Gate.power :heat,:ON,limit to 5000mA @14:08:41.19 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05084 @14:08:41.25 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @14:08:59.48 -> Cartridge.unlock @14:08:59.49 Gate.power :heat,:OFF @14:08:59.54 Gate.power :cartridge,:OFF @14:08:59.66 Carousel.in :default @14:08:59.81 Carousel.home.seek 56 @14:09:00.27 Air.in :unlocking @14:09:00.45 Air.seek :clear @14:09:01.89 Lysis1.in :unlocking @14:09:02.07 Lysis1.seek :clear @14:09:02.13 Plunge.in :unlocking @14:09:02.29 Plunge.seek :clear @14:09:12.79 -> Cartridge.deactivate @14:13:19.46 -> Cartridge.deactive @14:13:19.49 NoMethodError in bill -- undefined method `deactive' for Cartridge @14:13:32.01 -> Cartridge.deactivate @14:40:35.87 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:40:37.05 -> SC Storage Carousel (SC) at 56 @14:40:45.05 -> ESP.shutdown @14:40:45.76 Gate.power :motor,:OFF @14:40:45.79 Gate.power :core,:OFF @14:40:45.82 Gate.power :analytic1,:OFF @14:40:45.86 Gate.power :analytic2,:OFF @14:40:45.90 Gate.power :analytic3,:OFF @14:40:45.93 Gate.power :main,:OFF [:motor,:core,:analytic1,:analytic2,:analytic3,:main] @14:41:42.00 -> ESP.configure @14:41:42.04 Gate.power :main,:ON,limit to 5000mA @14:41:42.10 Gate.power :analytic1,:ON,limit to 5000mA @14:41:42.12 Gate.power :analytic2,:ON,limit to 5000mA @14:41:42.15 Gate.power :analytic3,:ON,limit to 5000mA @14:41:42.31 Powering core bus @14:41:44.61 SPR Module 1, FW Rev. B, FPGA Rev. C @14:41:45.07 Archive.configure :default @14:41:45.19 Air.configure :default @14:41:45.28 Lysis1.configure :default @14:41:45.38 Lysis2.configure :default @14:41:45.49 Plunge.configure :default @14:41:45.61 SamplePump.configure :default @14:41:45.73 Toroid.configure :default @14:41:45.85 Carousel.configure :default @14:41:45.97 Pressure.configure :default @14:41:46.09 ExtraPump.configure :default @14:41:46.18 PinPort.configure :default @14:41:46.27 All elf controllers running firmware version 4.83 @14:41:46.30 Gate.power :motor,:ON,limit to 5000mA @14:41:54.45 -> ESP.ready! @14:41:55.27 Archive.home.jog 0 @14:41:56.00 PinPort.home.jog 0 @14:41:56.08 Toroid.home.jog 0 @14:41:56.12 Lysis2.home.jog 0 @14:41:56.17 Lysis1.home.jog 0 @14:41:56.22 Plunge.home.jog 0 @14:41:56.26 Air.home.jog 0 @14:41:56.30 Carousel.home.jog 0 @14:41:57.29 Archive.in :homing @14:41:57.56 Archive.jog 1370 @14:41:57.63 PinPort.in :homing @14:41:57.68 Plunge.in :homing @14:41:57.74 Lysis1.in :homing @14:41:57.78 Air.in :homing @14:41:57.85 Toroid.in :homing @14:41:57.92 Lysis2.in :homing @14:41:58.25 PinPort.jog -107 @14:41:58.34 Lysis1.jog 1384 @14:41:58.41 Plunge.jog 375 @14:41:58.45 Air.jog 1383 @14:41:58.55 Toroid.jog 625 @14:41:58.63 Lysis2.jog 1382 @14:41:59.06 PinPort.setRawPosition! 0 @14:41:59.15 PinPort.seek :home @14:41:59.26 Archive.setRawPosition! 50 @14:41:59.34 Archive.seek :home @14:41:59.51 Plunge.setRawPosition! 50 @14:41:59.54 PinPort.in :default @14:41:59.68 Plunge.seek :home @14:41:59.82 Archive.in :default @14:41:59.93 Lysis1.setRawPosition! 50 @14:42:00.03 Lysis1.seek :home @14:42:00.18 Plunge.in :default @14:42:00.20 Lysis2.setRawPosition! 50 @14:42:00.24 Air.setRawPosition! 50 @14:42:00.41 Lysis2.seek :home @14:42:00.50 Air.seek :home @14:42:00.74 Lysis1.in :default @14:42:00.89 Lysis2.in :default @14:42:01.00 Toroid.setRawPosition! 0 @14:42:01.09 Air.in :default @14:42:01.15 Toroid.seek :home @14:42:01.33 Toroid.in :default @14:42:01.56 Toroid.seek :clear @14:42:03.20 Carousel.in :default @14:42:03.35 Carousel.home.seek 56 Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects blocked SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 56 Pressure = Absolute Pressure (Leak) Sensor @17psia PinPort = Handoff Pin Port (PP) clear @14:44:50.09 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:44:52.78 -> exit @14:44:52.80 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/methane/real.log @14:47:00.35 Session Begun @14:47:03.84 -> showlog {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:44:52.78 -> exit @14:44:52.80 Session Ended @14:47:00.35 Session Begun @14:47:03.84 -> showlog @14:47:50.13 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:48:02.46 -> showlog @14:47:03.84 -> showlog {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:44:52.78 -> exit @14:44:52.80 Session Ended @14:47:00.35 Session Begun @14:47:03.84 -> showlog @14:47:50.13 -> slot {55 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 56 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: "spr23mar2117h.spr.bz2"}} @14:48:02.46 -> showlog @14:48:56.69 -> esp.configure @14:48:56.72 NoMethodError in bill -- undefined method `configure' for :esp:Symbol @14:49:06.52 -> ESP.configure @14:49:06.72 Gate.power :analytic1,:ON,limit to 5000mA @14:49:06.74 Gate.power :analytic2,:ON,limit to 5000mA @14:49:06.77 Gate.power :analytic3,:ON,limit to 5000mA @14:49:07.55 Archive.configure :default @14:49:07.73 Air.configure :default @14:49:07.88 Lysis1.configure :default @14:49:08.05 Lysis2.configure :default @14:49:08.23 Plunge.configure :default @14:49:08.41 SamplePump.configure :default @14:49:08.60 Toroid.hold @14:49:08.76 Toroid.configure :default @14:49:08.95 Carousel.hold @14:49:09.06 Carousel.configure :default @14:49:09.24 Pressure.configure :default @14:49:09.43 ExtraPump.configure :default @14:49:09.59 PinPort.configure :default @14:49:09.67 All elf controllers running firmware version 4.83 @14:49:28.63 -> to 55 @14:49:28.97 Carousel.seek 55 @14:49:30.09 Lysis1.in :fast @14:49:30.18 Lysis1.seek :locked @14:49:32.83 Plunge.seek :locked @14:49:33.02 Air.in :fast @14:49:33.11 Air.seek :locked @14:49:34.71 Carousel.reconfigure :locked @14:49:34.91 Powering cartridge bus @14:49:37.56 Gate.setIR 1750,5000,0,6 @14:49:37.69 Gate.setIR 0,5000,0,6 @14:49:37.79 PuckHeater.configure :default @14:49:37.87 Gate.power :heat,:ON,limit to 5000mA @14:49:38.70 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @14:49:38.76 Using default cartridge heat loss curve for HeatingAccelInCarousel :daSPR_phyto @14:52:04.97 -> Cartridge.unlock @14:52:04.99 Gate.power :heat,:OFF @14:52:05.03 Gate.power :cartridge,:OFF @14:52:05.16 Carousel.in :default @14:52:05.32 Carousel.home.seek 55 @14:52:05.79 Air.in :unlocking @14:52:05.98 Air.seek :clear @14:52:07.41 Lysis1.in :unlocking @14:52:07.59 Lysis1.seek :clear @14:52:07.66 Plunge.in :unlocking @14:52:07.80 Plunge.seek :clear @14:52:15.31 -> exit @14:52:15.33 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/methane/real.log @14:52:50.95 Session Begun @14:52:53.79 -> showlog @14:52:15.31 -> exit @14:52:15.33 Session Ended @14:52:50.95 Session Begun @14:52:53.79 -> showlog @14:53:46.07 -> showlog @14:52:15.33 Session Ended @14:52:50.95 Session Begun @14:52:53.79 -> showlog @14:52:15.31 -> exit @14:52:15.33 Session Ended @14:52:50.95 Session Begun @14:52:53.79 -> showlog @14:53:46.07 -> showlog @14:53:57.06 -> MainThread.exit # @14:53:57.33 ThreadError in bill -- killed thread @14:53:57.41 Session Ended @14:56:14.69PDT22-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @14:56:14.72 >BREAK< ESP3 Gateway v1.42 for cartridge bus @14:56:19.30 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @14:56:20.04 Sea pressure (Abs) tare is 6% of full scale @14:56:23.83 Polling Can every 2:00 @14:56:23.88 Loading /home/methane/esp2/bin/processAll mission @14:56:24.14 Sea pressure (Abs) tare is 6% of full scale @14:56:24.16 Sampling default goal volumes @14:56:24.18 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @14:56:24.32 Gate.power :analytic1,:ON,limit to 5000mA @14:56:24.33 Gate.power :analytic2,:ON,limit to 5000mA @14:56:24.35 Gate.power :analytic3,:ON,limit to 5000mA @14:56:25.11 SPR Module 1, FW Rev. B, FPGA Rev. C @14:56:25.18
Archive.configure :default @14:56:25.24 Archive.hold @14:56:25.31 Archive.configure :default @14:56:25.46 Air.configure :default @14:56:25.52 Air.hold @14:56:25.59 Air.configure :default @14:56:25.72 Lysis1.configure :default @14:56:25.78 Lysis1.hold @14:56:25.85 Lysis1.configure :default @14:56:25.98 Lysis2.configure :default @14:56:26.05 Lysis2.hold @14:56:26.12 Lysis2.configure :default @14:56:26.27 Plunge.configure :default @14:56:26.33 Plunge.hold @14:56:26.40 Plunge.configure :default @14:56:26.57 SamplePump.configure :default @14:56:26.77 Toroid.configure :default @14:56:26.84 Toroid.hold @14:56:26.91 Toroid.configure :default @14:56:27.08 Carousel.configure :default @14:56:27.14 Carousel.hold @14:56:27.22 Carousel.configure :default @14:56:27.37 Pressure.configure :default @14:56:27.52 ExtraPump.configure :default @14:56:27.65 PinPort.configure :default @14:56:27.71 PinPort.hold @14:56:27.78 PinPort.configure :default @14:56:27.84 All elf controllers running firmware version 4.83 @14:56:28.66 Toroid.seek :clear @14:56:28.74 Carousel.in :default @14:56:28.88 Carousel.home.seek 55 @14:56:29.15 Selecting Cartridge 55 @14:56:29.46 Carousel.seek 55 @14:56:30.75 Lysis1.in :fast @14:56:30.82 Lysis1.seek :locked @14:56:33.45 Plunge.seek :locked @14:56:33.62 Air.in :fast @14:56:33.69 Air.seek :locked @14:56:35.27
Carousel.reconfigure :locked @14:56:35.44 Powering cartridge bus @14:56:38.09 Gate.setIR 1750,5000,0,6 @14:56:38.21 Gate.setIR 0,5000,0,6 @14:56:38.28 PuckHeater.configure :default @14:56:38.34 Gate.power :heat,:ON,limit to 5000mA @14:56:39.13 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @14:56:39.16 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:56:40.76 SPR.command "read_sequences" @14:56:41.07 SPR.command "stats_on" @14:56:41.22 SPR.command "start 0 1" @14:56:41.93 SPR.start /var/log/methane/spr23mar2214h.spr.bz2 @14:56:44.42 Waiting for baseline SPR stabilization @14:56:44.44 Handoff.select :waste @14:56:51.14 Syringe tare is 17% of full scale @14:56:51.16 PinPort.in :locking @14:56:51.22 PinPort.seek :locked @14:56:51.73 Archive.in :fast @14:56:51.79 Lysis2.in :fast @14:56:51.84 Lysis1.seek 1000uL @14:56:52.06 Lysis2.seek 1000uL @14:56:52.12 Archive.seek :full @14:56:56.69 delayUntil "15:06:44.39" @14:57:27.07 Session Begun @15:06:44.40 Lysis2.in :DAmix @15:06:44.56 Lysis1.in :DAmix @15:06:45.17 Lysis2.seek 750uL @15:06:45.23 Lysis1.seek 750uL @15:07:20.39 delay 5 seconds @15:07:25.41 Handoff.select SPR @15:07:25.56 SPR.command "start 2 14" @15:07:26.13 SPR stabilized @15:07:26.34 delay 10 seconds @15:07:36.36 SPR.command "start 1 0" @15:07:39.51 Waiting up to 4:00 for SPR to enter state 1 @15:09:43.78 SPR.command "hold_on" @15:09:45.19 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @15:09:45.83 Lysis2.seek 450uL @15:09:45.89 Lysis1.seek 450uL @15:10:28.01 delay 15 seconds @15:10:43.03 SPR.command "hold_off" @15:10:45.84 Waiting up to 38:00 for SPR to enter state 7 @15:22:31.97 Session Ended @15:28:40.72 SPRsummary:91.3707,192.635,217.839,278.412 after 2001s @15:35:18.27 delay 10 seconds @15:35:28.32 SPR.command "start 1 0" @15:35:32.26 Waiting up to 4:00 for SPR to enter state 1 @15:37:36.97 SPR.command "hold_on" @15:37:38.36 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:37:38.97 Lysis2.seek 300uL @15:37:39.03 Lysis1.seek 300uL @15:38:00.16 delay 15 seconds @15:38:15.18 SPR.command "hold_off" @15:38:18.21 Waiting up to 38:00 for SPR to enter state 7 @16:02:50.47 delay 10 seconds @16:03:00.49 SPR.command "start 1 0" @16:03:03.17 Waiting up to 4:00 for SPR to enter state 1 @16:05:07.45 SPR.command "hold_on" @16:05:08.84 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:05:09.41 Lysis2.seek 150uL @16:05:09.45 Lysis1.seek 150uL @16:05:30.55 delay 15 seconds @16:05:45.57 SPR.command "hold_off" @16:05:48.68 Waiting up to 38:00 for SPR to enter state 7 @16:30:20.93 delay 10 seconds @16:30:30.95 SPR.command "start 1 0" @16:30:33.65 Waiting up to 4:00 for SPR to enter state 1 @16:32:37.93 SPR.command "hold_on" @16:32:39.32 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:32:39.90 Lysis2.seek 0uL @16:32:39.96 Lysis1.seek 0uL @16:33:01.07 delay 15 seconds @16:33:16.09 SPR.command "hold_off" @16:33:19.17 Waiting up to 38:00 for SPR to enter state 7 @16:57:51.28 SPR analysis completed @16:57:53.21 Ended log spr23mar2214h.spr.bz2 @16:57:53.22 SPR.command "stats_off" @16:57:53.76 Intake.close @16:57:53.83 Exhaust.close @16:57:53.91 Bypass.close @16:57:54.01 Handoff.select :close @16:57:54.21
Gate.power :heat,:OFF @16:57:54.22 Gate.power :cartridge,:OFF @16:57:54.31 PinPort.in :unlocking @16:57:54.37 PinPort.seek :clear @16:57:54.84 Carousel.in :default @16:57:54.97 Carousel.home.seek 55 @16:57:55.30 Plunge.seek :locked @16:57:55.48 Archive.in :unlocking @16:57:55.63 Archive.seek :clear @16:57:55.72 Air.in :unlocking @16:57:55.89 Air.seek :clear @16:57:55.94 Lysis1.in :unlocking @16:57:56.12 Lysis1.seek :clear @16:57:56.22 Lysis2.in :unlocking @16:57:56.29 Lysis2.seek :clear @16:58:01.41
Gate.power :motor,:OFF @16:58:01.43 Gate.power :core,:OFF @16:58:01.44 Gate.power :analytic1,:OFF @16:58:01.45 Gate.power :analytic2,:OFF @16:58:01.47 Gate.power :analytic3,:OFF @16:58:01.48 Gate.power :main,:OFF @16:58:01.49 Safely stopped and ready to power off @16:58:01.97 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:56:20.36PDT23-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:56:20.38 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:56:25.11 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:56:25.88 Sea pressure (Abs) tare is 6% of full scale @13:56:29.77 Polling Can every 2:00 @13:56:29.82 Loading /home/methane/esp2/bin/processAll mission @13:56:30.20 Sea pressure (Abs) tare is 6% of full scale @13:56:30.23 Sampling default goal volumes @13:56:30.25 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:56:30.42 Gate.power :analytic1,:ON,limit to 5000mA @13:56:30.43 Gate.power :analytic2,:ON,limit to 5000mA @13:56:30.44 Gate.power :analytic3,:ON,limit to 5000mA @13:56:31.17 Archive.configure :default @13:56:31.33 Air.configure :default @13:56:31.49 SPR Module 1, FW Rev. B, FPGA Rev. C @13:56:31.52
Lysis1.configure :default @13:56:31.68 Lysis2.configure :default @13:56:31.83 Plunge.configure :default @13:56:31.99 SamplePump.configure :default @13:56:32.14 Toroid.configure :default @13:56:32.20 Toroid.hold @13:56:32.27 Toroid.configure :default @13:56:32.43 Carousel.configure :default @13:56:32.48 Carousel.hold @13:56:32.56 Carousel.configure :default @13:56:32.71 Pressure.configure :default @13:56:32.87 ExtraPump.configure :default @13:56:33.01 PinPort.configure :default @13:56:33.07 All elf controllers running firmware version 4.83 @13:56:33.95 Toroid.seek :clear @13:56:34.03 Carousel.in :default @13:56:34.17 Carousel.home.seek 56 @13:56:34.42 Selecting Cartridge 55 @13:56:34.74 Carousel.seek 55 @13:56:35.90 Lysis1.in :fast @13:56:35.96 Lysis1.seek :locked @13:56:39.01 Plunge.seek :locked @13:56:39.23 Air.in :fast @13:56:39.29 Air.seek :locked @13:56:40.87
Carousel.reconfigure :locked @13:56:41.04 Powering cartridge bus @13:56:43.69 Gate.setIR 1750,5000,0,6 @13:56:43.82 Gate.setIR 0,5000,0,6 @13:56:43.89 PuckHeater.configure :default @13:56:43.95 Gate.power :heat,:ON,limit to 5000mA @13:56:44.75 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:56:44.78 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:56:46.34 SPR.command "read_sequences" @13:56:46.53 SPR.command "stats_on" @13:56:46.69 SPR.command "start 0 1" @13:56:47.39 SPR.start /var/log/methane/spr23mar2313h.spr.bz2 @13:56:49.39 Waiting for baseline SPR stabilization @13:56:49.41 Handoff.select :waste @13:56:56.11 Syringe tare is 13% of full scale @13:56:56.13 PinPort.in :locking @13:56:56.20 PinPort.seek :locked @13:56:56.69 Archive.in :fast @13:56:56.76 Lysis2.in :fast @13:56:56.81 Lysis1.seek 1000uL @13:56:56.98 Lysis2.seek 1000uL @13:56:57.03 Archive.seek :full @13:57:01.61 delayUntil "14:06:48.93" @13:58:35.00 Session Begun @13:58:40.81 -> Can Can@13:58:30, 9.2C, 21% humidity, 14.8psia, 14.916V, 0.524A, 0.197Ah, 7.81W @14:06:48.94 Lysis2.in :DAmix @14:06:49.10 Lysis1.in :DAmix @14:06:49.71 Lysis2.seek 750uL @14:06:49.77 Lysis1.seek 750uL @14:07:24.95 delay 5 seconds @14:07:29.97 Handoff.select SPR @14:07:30.13 SPR.command "start 2 14" @14:07:32.10 SPR stabilized @14:07:32.28 delay 10 seconds @14:07:42.30 SPR.command "start 1 0" @14:07:44.97 Waiting up to 4:00 for SPR to enter state 1 @14:09:49.23 SPR.command "hold_on" @14:09:50.64 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:09:51.28 Lysis2.seek 450uL @14:09:51.35 Lysis1.seek 450uL @14:10:33.51 delay 15 seconds @14:10:48.53 SPR.command "hold_off" @14:10:51.79 Waiting up to 38:00 for SPR to enter state 7 @14:28:47.10 SPRsummary:80.8973,193.197,235.899,293.405 after 2002s @14:35:24.48 delay 10 seconds @14:35:34.50 SPR.command "start 1 0" @14:35:37.19 Waiting up to 4:00 for SPR to enter state 1 @14:37:41.45 SPR.command "hold_on" @14:37:42.87 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:37:43.49 Lysis2.seek 300uL @14:37:43.55 Lysis1.seek 300uL @14:38:04.76 delay 15 seconds @14:38:19.78 SPR.command "hold_off" @14:38:23.21 Waiting up to 38:00 for SPR to enter state 7 @14:45:04.13 Session Ended @15:02:55.88 delay 10 seconds @15:03:05.91 SPR.command "start 1 0" @15:03:08.61 Waiting up to 4:00 for SPR to enter state 1 @15:05:12.88 SPR.command "hold_on" @15:05:14.29 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:05:14.84 Lysis2.seek 150uL @15:05:14.90 Lysis1.seek 150uL @15:05:36.00 delay 15 seconds @15:05:51.02 SPR.command "hold_off" @15:05:54.13 Waiting up to 38:00 for SPR to enter state 7 @15:30:26.35 delay 10 seconds @15:30:36.37 SPR.command "start 1 0" @15:30:39.09 Waiting up to 4:00 for SPR to enter state 1 @15:32:43.34 SPR.command "hold_on" @15:32:45.26 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:32:45.81 Lysis2.seek 0uL @15:32:45.86 Lysis1.seek 0uL @15:33:07.00 delay 15 seconds @15:33:22.04 SPR.command "hold_off" @15:33:24.61 Waiting up to 38:00 for SPR to enter state 7 @15:57:55.73 SPR analysis completed @15:57:57.56 Ended log spr23mar2313h.spr.bz2 @15:57:57.58 SPR.command "stats_off" @15:57:58.26 Intake.close @15:57:58.30 Exhaust.close @15:57:58.39 Bypass.close @15:57:58.45 Handoff.select :close @15:57:59.18
Gate.power :heat,:OFF @15:57:59.19 Gate.power :cartridge,:OFF @15:57:59.29 PinPort.in :unlocking @15:57:59.35 PinPort.seek :clear @15:57:59.82 Carousel.in :default @15:57:59.95 Carousel.home.seek 55 @15:58:00.30 Plunge.seek :locked @15:58:00.47 Archive.in :unlocking @15:58:00.61 Archive.seek :clear @15:58:00.71 Air.in :unlocking @15:58:00.86 Air.seek :clear @15:58:00.99 Lysis1.in :unlocking @15:58:01.12 Lysis1.seek :clear @15:58:01.21 Lysis2.in :unlocking @15:58:01.31 Lysis2.seek :clear @15:58:06.43
Gate.power :motor,:OFF @15:58:06.44 Gate.power :core,:OFF @15:58:06.45 Gate.power :analytic1,:OFF @15:58:06.47 Gate.power :analytic2,:OFF @15:58:06.48 Gate.power :analytic3,:OFF @15:58:06.49 Gate.power :main,:OFF @15:58:06.51 Safely stopped and ready to power off @15:58:07.14 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:09:33.97PDT24-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:09:33.99 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:09:40.70 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:09:41.47 Sea pressure (Abs) tare is 6% of full scale @13:09:45.75 Polling Can every 2:00 @13:09:45.80 Loading /home/methane/esp2/bin/processAll mission @13:09:46.09 Sea pressure (Abs) tare is 6% of full scale @13:09:46.10 Sampling default goal volumes @13:09:46.12 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:09:46.26 Gate.power :analytic1,:ON,limit to 5000mA @13:09:46.30 Gate.power :analytic2,:ON,limit to 5000mA @13:09:46.32 Gate.power :analytic3,:ON,limit to 5000mA @13:09:47.09 Archive.configure :default @13:09:47.24 Air.configure :default @13:09:47.36 Lysis1.configure :default @13:09:47.49 Lysis2.configure :default @13:09:47.70 SPR Module 1, FW Rev. B, FPGA Rev. C @13:09:47.73
Plunge.configure :default @13:09:47.93 SamplePump.configure :default @13:09:48.08 Toroid.configure :default @13:09:48.23 Carousel.configure :default @13:09:48.38 Pressure.configure :default @13:09:48.53 ExtraPump.configure :default @13:09:48.66 PinPort.configure :default @13:09:48.72 All elf controllers running firmware version 4.83 @13:09:48.95 Archive.in :default @13:09:49.33 Archive.home.jog 0 @13:09:49.62 PinPort.in :default @13:09:49.70 Carousel.in :default @13:09:49.73 Toroid.in :default @13:09:49.79 Lysis1.in :default @13:09:49.82 Air.in :default @13:09:49.89 Plunge.in :default @13:09:49.96 Lysis2.in :default @13:09:50.24 Carousel.home.jog 0 @13:09:50.33 PinPort.home.jog 0 @13:09:50.35 Lysis1.home.jog 0 @13:09:50.38 Toroid.home.jog 0 @13:09:50.41 Lysis2.home.jog 0 @13:09:50.45 Air.home.jog 0 @13:09:50.50 Plunge.home.jog 0 @13:09:51.26 Archive.in :homing @13:09:51.48 Archive.jog 1389 @13:09:51.55 PinPort.in :homing @13:09:51.58 Plunge.in :homing @13:09:51.69 Lysis1.in :homing @13:09:51.73 Toroid.in :homing @13:09:51.76 Air.in :homing @13:09:51.80 Lysis2.in :homing @13:09:52.12 Plunge.jog 382 @13:09:52.17 PinPort.jog -107 @13:09:52.19 Lysis1.jog 1389 @13:09:52.22 Toroid.jog 617 @13:09:52.26 Air.jog 1373 @13:09:52.29 Lysis2.jog 1393 @13:09:53.16 Plunge.setRawPosition! 50 @13:09:53.20 Archive.setRawPosition! 50 @13:09:53.22 PinPort.setRawPosition! 0 @13:09:53.34 Plunge.seek :home @13:09:53.39 Archive.seek :home @13:09:53.42 PinPort.seek :home @13:09:53.69 Lysis1.setRawPosition! 50 @13:09:53.72 PinPort.in :default @13:09:53.83 Lysis1.seek :home @13:09:53.99 Archive.in :default @13:09:54.02 Plunge.in :default @13:09:54.21 Lysis1.in :default @13:09:54.23 Air.setRawPosition! 50 @13:09:54.26 Lysis2.setRawPosition! 50 @13:09:54.43 Lysis2.seek :home @13:09:54.45 Air.seek :home @13:09:54.76 Air.in :default @13:09:54.85 Lysis2.in :default @13:09:54.97 Toroid.setRawPosition! 0 @13:09:55.01 Toroid.seek :home @13:09:55.10 Toroid.in :default @13:09:55.20
Toroid.seek :clear @13:09:56.81 Carousel.in :default @13:09:56.94 Carousel.home.seek 54 @13:09:57.38 Selecting Cartridge 55 @13:09:57.73 Carousel.seek 55 @13:09:58.82 Lysis1.in :fast @13:09:58.88 Lysis1.seek :locked @13:10:01.51 Plunge.seek :locked @13:10:01.70 Air.in :fast @13:10:01.76 Air.seek :locked @13:10:03.35
Carousel.reconfigure :locked @13:10:03.51 Powering cartridge bus @13:10:06.21 Gate.setIR 1750,5000,0,6 @13:10:06.35 Gate.setIR 0,5000,0,6 @13:10:06.48 PuckHeater.configure :default @13:10:06.60 Gate.power :heat,:ON,limit to 5000mA @13:10:07.48 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:10:07.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:10:09.09 SPR.command "read_sequences" @13:10:09.34 SPR.command "stats_on" @13:10:10.55 SPR.command "start 0 1" @13:10:11.10 SPR.start /var/log/methane/spr23mar2413h.spr.bz2 @13:10:13.14 Waiting for baseline SPR stabilization @13:10:13.15 Handoff.select :waste @13:10:19.85 Syringe tare is 16% of full scale @13:10:19.87 PinPort.in :locking @13:10:19.94 PinPort.seek :locked @13:10:20.42 Archive.in :fast @13:10:20.48 Lysis2.in :fast @13:10:20.58 Lysis1.seek 1000uL @13:10:20.70 Archive.seek :full @13:10:20.77 Lysis2.seek 1000uL @13:10:25.34 delayUntil "13:20:12.68" @13:20:12.69 Lysis2.in :DAmix @13:20:12.82 Lysis1.in :DAmix @13:20:13.32 Lysis2.seek 750uL @13:20:13.39 Lysis1.seek 750uL @13:20:48.56 delay 5 seconds @13:20:53.58 Handoff.select SPR @13:20:53.74 SPR.command "start 2 14" @13:20:54.42 SPR stabilized @13:20:54.58 delay 10 seconds @13:21:04.60 SPR.command "start 1 0" @13:21:07.77 Waiting up to 4:00 for SPR to enter state 1 @13:23:12.04 SPR.command "hold_on" @13:23:13.45 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @13:23:14.07 Lysis2.seek 450uL @13:23:14.13 Lysis1.seek 450uL @13:23:56.25 delay 15 seconds @13:24:11.27 SPR.command "hold_off" @13:24:14.10 Waiting up to 38:00 for SPR to enter state 7 @13:42:08.95 SPRsummary:68.0947,231.824,285.704,370.457 after 2000s @13:48:46.32 delay 10 seconds @13:48:56.34 SPR.command "start 1 0" @13:48:59.06 Waiting up to 4:00 for SPR to enter state 1 @13:51:03.32 SPR.command "hold_on" @13:51:04.73 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:51:05.32 Lysis2.seek 300uL @13:51:05.40 Lysis1.seek 300uL @13:51:26.48 delay 15 seconds @13:51:41.50 SPR.command "hold_off" @13:51:44.57 Waiting up to 38:00 for SPR to enter state 7 @14:16:17.34 delay 10 seconds @14:16:27.36 SPR.command "start 1 0" @14:16:30.47 Waiting up to 4:00 for SPR to enter state 1 @14:18:34.74 SPR.command "hold_on" @14:18:36.15 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:18:36.70 Lysis2.seek 150uL @14:18:36.75 Lysis1.seek 150uL @14:18:57.87 delay 15 seconds @14:19:12.89 SPR.command "hold_off" @14:19:15.99 Waiting up to 38:00 for SPR to enter state 7 @14:43:48.22 delay 10 seconds @14:43:58.24 SPR.command "start 1 0" @14:44:00.94 Waiting up to 4:00 for SPR to enter state 1 @14:46:05.21 SPR.command "hold_on" @14:46:07.12 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:46:07.67 Lysis2.seek 0uL @14:46:07.71 Lysis1.seek 0uL @14:46:28.84 delay 15 seconds @14:46:43.87 SPR.command "hold_off" @14:46:46.47 Waiting up to 38:00 for SPR to enter state 7 @15:11:17.62 SPR analysis completed @15:11:19.51 Ended log spr23mar2413h.spr.bz2 @15:11:19.53 SPR.command "stats_off" @15:11:20.60 Intake.close @15:11:20.66 Exhaust.close @15:11:20.76 Bypass.close @15:11:20.79 Handoff.select :close @15:11:21.05
Gate.power :heat,:OFF @15:11:21.06 Gate.power :cartridge,:OFF @15:11:21.14 PinPort.in :unlocking @15:11:21.20 PinPort.seek :clear @15:11:21.67 Carousel.in :default @15:11:21.81 Carousel.home.seek 55 @15:11:22.14 Plunge.seek :locked @15:11:22.31 Archive.in :unlocking @15:11:22.46 Archive.seek :clear @15:11:22.55 Air.in :unlocking @15:11:22.71 Air.seek :clear @15:11:22.76 Lysis1.in :unlocking @15:11:22.95 Lysis1.seek :clear @15:11:23.05 Lysis2.in :unlocking @15:11:23.13 Lysis2.seek :clear @15:11:28.28
Gate.power :motor,:OFF @15:11:28.30 Gate.power :core,:OFF @15:11:28.31 Gate.power :analytic1,:OFF @15:11:28.32 Gate.power :analytic2,:OFF @15:11:28.34 Gate.power :analytic3,:OFF @15:11:28.35 Gate.power :main,:OFF @15:11:28.36 Safely stopped and ready to power off @15:11:28.80 Cartridge::None in MAIN -- Not enough standard cartridges remain @17:14:37.44PDT24-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:14:37.47 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:14:43.07 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:14:43.90 Sea pressure (Abs) tare is 6% of full scale @17:14:48.03 Polling Can every 2:00 @17:14:48.07 Loading /home/methane/esp2/bin/processAll mission @17:14:48.35 Sea pressure (Abs) tare is 6% of full scale @17:14:48.36 Sampling default goal volumes @17:14:48.37 Cartridge group: dry @17:14:48.52 Gate.power :analytic1,:ON,limit to 5000mA @17:14:48.54 Gate.power :analytic2,:ON,limit to 5000mA @17:14:48.58 Gate.power :analytic3,:ON,limit to 5000mA @17:14:49.55 Archive.configure :default @17:14:49.72 SPR Module 1, FW Rev. B, FPGA Rev. C @17:14:49.80
Air.configure :default @17:14:49.86 Air.hold @17:14:49.92 Air.configure :default @17:14:50.05 Lysis1.configure :default @17:14:50.10 Lysis1.hold @17:14:50.17 Lysis1.configure :default @17:14:50.31 Lysis2.configure :default @17:14:50.46 Plunge.configure :default @17:14:50.52 Plunge.hold @17:14:50.59 Plunge.configure :default @17:14:50.73 SamplePump.configure :default @17:14:50.88 Toroid.configure :default @17:14:50.94 Toroid.hold @17:14:51.01 Toroid.configure :default @17:14:51.16 Carousel.configure :default @17:14:51.22 Carousel.hold @17:14:51.28 Carousel.configure :default @17:14:51.43 Pressure.configure :default @17:14:51.58 ExtraPump.configure :default @17:14:51.74 PinPort.configure :default @17:14:51.80 All elf controllers running firmware version 4.83 @17:14:52.62 Toroid.seek :clear @17:14:52.69 Carousel.in :default @17:14:52.82 Carousel.home.seek 56 @17:14:53.09 Selecting Cartridge 56 @17:14:53.39 Carousel.seek 56 @17:14:54.63 Lysis1.in :fast @17:14:54.70 Lysis1.seek :locked @17:14:57.32 Plunge.seek :locked @17:14:57.49 Air.in :fast @17:14:57.56 Air.seek :locked @17:14:59.13
Carousel.reconfigure :locked @17:14:59.28 Powering cartridge bus @17:15:01.92 Gate.setIR 1750,5000,0,6 @17:15:02.04 Gate.setIR 0,5000,0,6 @17:15:02.11 PuckHeater.configure :default @17:15:02.17 Gate.power :heat,:ON,limit to 5000mA @17:15:02.91 Mounted lysis Cartridge board#Q05054 @17:15:02.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:15:03.26 Preparing to filter cartridge 56 @17:15:03.59 Priming sample loop w/100ml, bypass w/10ml @17:15:03.61 SamplePump.setPosition! 0ml @17:15:03.68 Exhaust.close @17:15:03.76 Bypass.open @17:15:05.19 Intake.open @17:15:05.90 Sea 5.8m deep (23.3psia) @17:15:05.93 Waiting up to 20 seconds for sea pressure to stabilize +/-0.50psi @17:15:08.51 Vacuum tare is 60% of full scale @17:15:08.66 Pressure.in :default @17:15:08.72 Pressure.hold @17:15:08.94 SamplePump.in :prime @17:15:09.01 SamplePump.seek 10ml,59 seconds @17:15:17.75 Pressure.stop @17:15:17.81 SamplePump.setPosition! 0ml @17:15:17.86 Exhaust.open @17:15:19.28 Bypass.close @17:15:20.80 Pressure.in :default @17:15:20.86 Pressure.hold @17:15:21.00 Sea 6.0m deep (23.5psia) @17:15:21.17 SamplePump.in :prime @17:15:21.24 SamplePump.seek 100ml,5:16 @17:16:55.92 Pressure.stop @17:16:56.37 Bypass.open @17:16:57.16 Intake.close @17:16:57.94 Toroid.in :hold @17:16:58.00 Toroid.seek :crack @17:16:59.54 Toroid.seek :cartridge @17:17:00.66 Toroid.in :default @17:17:00.72 Toroid.seek :clear @17:17:02.73 Pressurizing cartridge to 26psi above 23.2psia @17:17:02.75 SamplePump.setPosition! 0ml @17:17:02.79 Intake.open @17:17:03.50 Bypass.close @17:17:04.18 Exhaust.close @17:17:05.02 Pressure.in :default @17:17:05.08 Pressure.hold @17:17:05.31 SamplePump.in :charge @17:17:05.38 SamplePump.seek 2.3ml,42 seconds @17:17:20.39 Pressure.stop @17:17:20.45 Intake.close @17:17:21.22 Pressurized cartridge with 1.070ml to 26.1psi @17:17:21.23 Isolated @47.3psia (24.1psi) @17:17:21.25 Verifying cartridge holds at least 0.65..0.85 of 47.3psia @17:17:21.27 Waiting up to 20 seconds for cartridge pressure to stabilize +/-1.50psi @17:17:25.87 Cartridge pressure fell 1% to 46.9psia @17:17:25.97 Sampling 1000.0ml in 1:25:20 @17:17:25.99 SamplePump.setPosition! 0ml @17:17:26.04 Exhaust.open @17:17:26.77 Intake.open @17:17:27.59 Pressure.in :default @17:17:27.69 Pressure.hold @17:17:27.82 Sea 7.5m deep (25.8psia) @17:17:27.99 SamplePump.in :sample @17:17:28.06 SamplePump.seek 1000ml,1:23:51 @17:17:30.64 Cartridge 56 filtering 1/1000ml at 0.63ml/s with 25.9psi vacuum @17:18:30.66 Cartridge 56 filtering 39/1000ml at 0.65ml/s with 25.1psi vacuum 5.8m deep (23.3psia) @17:19:30.65 Cartridge 56 filtering 77/1000ml at 0.64ml/s with 23.6psi vacuum 5.3m deep (22.5psia) @17:20:30.65 Cartridge 56 filtering 115/1000ml at 0.64ml/s with 23.4psi vacuum 6.0m deep (23.6psia) @17:21:30.66 Cartridge 56 filtering 153/1000ml at 0.64ml/s with 24.4psi vacuum 3.9m deep (20.5psia) @17:22:31.05 Cartridge 56 filtering 190/1000ml at 0.63ml/s with 25.3psi vacuum 3.5m deep (19.8psia) @17:23:30.66 Cartridge 56 filtering 227/1000ml at 0.61ml/s with 26.5psi vacuum 3.1m deep (19.3psia) @17:24:30.65 Cartridge 56 filtering 264/1000ml at 0.60ml/s with 18.9psi vacuum 6.2m deep (23.8psia) @17:25:30.67 Cartridge 56 filtering 300/1000ml at 0.59ml/s with 24.6psi vacuum 4.3m deep (21.1psia) @17:26:30.65 Cartridge 56 filtering 337/1000ml at 0.63ml/s with 22.1psi vacuum 6.5m deep (24.3psia) @17:27:30.66 Cartridge 56 filtering 373/1000ml at 0.59ml/s with 19.0psi vacuum 6.2m deep (23.9psia) @17:28:30.66 Cartridge 56 filtering 409/1000ml at 0.61ml/s with 22.4psi vacuum 5.9m deep (23.3psia) @17:29:30.66 Cartridge 56 filtering 444/1000ml at 0.58ml/s with 25.0psi vacuum 3.6m deep (20.0psia) @17:30:30.65 Cartridge 56 filtering 480/1000ml at 0.58ml/s with 24.4psi vacuum @17:31:30.65 Cartridge 56 filtering 516/1000ml at 0.58ml/s with 26.1psi vacuum 3.8m deep (20.3psia) @17:32:30.66 Cartridge 56 filtering 551/1000ml at 0.58ml/s with 21.0psi vacuum 3.9m deep (20.4psia) @17:33:30.65 Cartridge 56 filtering 586/1000ml at 0.60ml/s with 20.1psi vacuum 5.9m deep (23.3psia) @17:34:30.66 Cartridge 56 filtering 622/1000ml at 0.58ml/s with 24.1psi vacuum 4.4m deep (21.2psia) @17:35:30.65 Cartridge 56 filtering 657/1000ml at 0.60ml/s with 26.6psi vacuum 4.7m deep (21.7psia) @17:36:30.66 Cartridge 56 filtering 692/1000ml at 0.56ml/s with 25.4psi vacuum 3.1m deep (19.2psia) @17:37:30.65 Cartridge 56 filtering 727/1000ml at 0.58ml/s with 24.3psi vacuum 2.8m deep (18.8psia) @17:38:30.66 Cartridge 56 filtering 762/1000ml at 0.56ml/s with 25.0psi vacuum 3.3m deep (19.6psia) @17:39:30.65 Cartridge 56 filtering 796/1000ml at 0.60ml/s with 25.4psi vacuum 4.4m deep (21.2psia) @17:40:30.65 Cartridge 56 filtering 831/1000ml at 0.59ml/s with 21.0psi vacuum 6.7m deep (24.6psia) @17:41:30.65 Cartridge 56 filtering 866/1000ml at 0.56ml/s with 25.2psi vacuum 3.4m deep (19.8psia) @17:42:30.65 Cartridge 56 filtering 901/1000ml at 0.56ml/s with 25.0psi vacuum 3.5m deep (19.9psia) @17:43:30.66 Cartridge 56 filtering 936/1000ml at 0.60ml/s with 21.1psi vacuum 6.8m deep (24.7psia) @17:44:30.65 Cartridge 56 filtering 970/1000ml at 0.60ml/s with 24.3psi vacuum 5.7m deep (23.2psia) @17:45:22.12 Pressure.stop @17:45:22.26 Intake.close @17:45:22.30 Exhaust.close @17:45:22.57 Toroid.in :hold @17:45:22.63 Toroid.seek :bypass @17:45:24.26 Toroid.in :default @17:45:24.32 Toroid.seek :clear @17:45:25.94 Sampled 1000.0ml @17:45:27.57 SPR.command "read_sequences" @17:45:28.30 SPR.command "stats_on" @17:45:28.44 SPR.command "start 0 1" @17:45:29.16 SPR.start /var/log/methane/spr23mar2417h.spr.bz2 @17:45:31.24 Handoff.select :waste @17:45:37.93 Syringe tare is 16% of full scale @17:45:37.96 PinPort.in :locking @17:45:38.02 PinPort.seek :locked @17:45:38.50 Archive.in :fast @17:45:38.57 Lysis2.in :fast @17:45:38.65 Lysis1.seek 1350uL @17:45:38.75 Archive.seek :full @17:45:38.83 Lysis2.seek :full @17:45:40.80 Plunge.seek :reagent @17:45:41.72 Evacuating puck with filter bubble point of 50psi @17:45:41.75 PinPort.seek :locked @17:45:41.82 Handoff.select :waste @17:45:48.43 Air syringe pumping up to 1 strokes to reach 8psi @17:45:48.44 Air stroke #1 of 1 @17:45:48.53 Air.in :pressurize @17:45:48.59 Air.seek :pressurized @17:45:54.42 2.2psi after pressurize @17:45:54.44 Air.in :compress @17:45:54.50 Air.seek :evac @17:45:57.52 1.5psi after compress @17:46:09.65 0.6psi after compress (stable) @17:46:09.67 Air.in :default @17:46:09.72 Air.hold @17:46:09.94 at 0.6psi Opened to waste @17:46:09.95 Handoff.select :blocked @17:46:10.08 Archive syringe pumping up to 8 strokes to reach 50psi @17:46:10.09 Archive stroke #1 of 8 @17:46:10.18 Archive.in :pressurize @17:46:10.24 Archive.seek :pressurized @17:46:22.79 1.6psi after pressurize @17:46:28.43 1.7psi after pressurize (stable) @17:46:28.46 Archive.in :compress @17:46:28.54 Archive.seek :empty @17:46:43.18 13.0psi after compress @17:46:55.27 17.8psi after compress (stable) @17:46:55.29 Archive.in :gulp @17:46:55.35 Archive.seek :topVent @17:47:01.62 18.1psi after gulp @17:47:07.63 18.0psi after gulp (stable) @17:47:07.74 Archive stroke #2 of 8 @17:47:07.82 Archive.in :pressurize @17:47:07.89 Archive.seek :pressurized @17:47:20.42 17.9psi after pressurize @17:47:26.03 17.8psi after pressurize (stable) @17:47:26.08 Archive.in :compress @17:47:26.17 Archive.seek :empty @17:47:40.80 31.8psi after compress @17:47:52.39 34.3psi after compress (stable) @17:47:52.42 Archive.in :gulp @17:47:52.47 Archive.seek :topVent @17:47:58.75 34.4psi after gulp @17:48:04.34 34.4psi after gulp (stable) @17:48:04.36 Archive stroke #3 of 8 @17:48:04.44 Archive.in :pressurize @17:48:04.51 Archive.seek :pressurized @17:48:17.01 34.2psi after pressurize @17:48:22.61 34.0psi after pressurize (stable) @17:48:22.63 Archive.in :compress @17:48:22.69 Archive.seek :empty @17:48:37.72 47.5psi after compress @17:48:48.43 49.7psi after compress (stable) @17:48:48.46 Archive.in :gulp @17:48:48.52 Archive.seek :topVent @17:48:49.12 Reached 50.1 of 50.0psi after pumping 5190uL (3.00 strokes) @17:48:49.14 Archive.in :default @17:48:49.20 Archive.hold @17:48:49.29 Handoff.select :waste @17:48:57.02 delay 1:00 @17:49:57.04 Handoff.select :blocked @17:50:19.74 Handoff.select :waste @17:50:19.86 0.9psi at evacEnd @17:50:19.88 Handoff.select :blocked @17:50:20.00 Archive.in :fast @17:50:20.08 Archive.seek :topVent @17:50:33.46 Stable @1.0psi after Handoff blocked @17:50:33.49 Archive syringe pumping up to 8 strokes to reach 31psi @17:50:33.50 Archive stroke #1 of 8 @17:50:33.58 Archive.in :pressurize @17:50:33.67 Archive.seek :pressurized @17:50:46.18 1.0psi after pressurize @17:50:46.20 Archive.in :compress @17:50:46.26 Archive.seek :empty @17:51:00.90 14.2psi after compress @17:51:12.49 16.3psi after compress (stable) @17:51:12.52 Archive.in :gulp @17:51:12.58 Archive.seek :topVent @17:51:18.85 16.4psi after gulp @17:51:24.50 16.4psi after gulp (stable) @17:51:24.51 Archive stroke #2 of 8 @17:51:24.60 Archive.in :pressurize @17:51:24.66 Archive.seek :pressurized @17:51:37.16 16.4psi after pressurize @17:51:37.19 Archive.in :compress @17:51:37.25 Archive.seek :empty @17:51:51.94 29.3psi after compress @17:51:55.68 Reached 31.0 of 31.0psi after pumping 3460uL (2.00 strokes) @17:51:55.71 Archive.in :default @17:51:55.77 Archive.hold @17:51:55.85 Handoff.select :waste @17:52:03.06 delay 1:00 @17:53:03.08 Handoff.select :blocked @17:53:25.78 Handoff.select :waste @17:53:25.90 0.4psi at evacEnd @17:53:25.92 Air.seek :fullVent @17:53:27.35 Archive.in :fast @17:53:27.47 Archive.seek :topVent @17:53:34.58 Seawater evacuated, Pressure above/below filter released. @17:53:34.67 Lysis1.in :slow @17:53:34.73 Lysis1.seek 1050uL @17:54:04.76 delay 5 seconds @17:54:09.78 Handoff.select :blocked @17:54:10.02 Lysis1.seek 450uL @17:55:09.99 delay 3 seconds @17:55:13.08 Air.in mix with 1.40048 parts Lysis1 @17:55:13.73 Air.seek :evac @17:55:13.77 Lysis1.seek 300uL @17:55:28.90 Lysis1.seek :empty @17:55:59.01 Lysis buffer delivered @23.9psi @17:55:59.03 Polling Can every 2:00 @17:55:59.57 PuckHeater.configure :default #ambient 13.1C @17:55:59.66 PuckHeater.seek 80.1C @17:56:47.34 PuckHeater.hold 2:00,80.1C @17:58:47.38 Polling Can every 2:00 @17:58:47.93 PuckHeater.configure :default #ambient 13.1C @17:58:48.00 PuckHeater.hold 30 seconds,79.1C @17:59:18.23 Polling Can every 2:00 @17:59:18.79 PuckHeater.hold 30 seconds,78.6C @17:59:48.98 Polling Can every 2:00 @17:59:49.53 PuckHeater.configure :default #ambient 13.1C @17:59:49.59 PuckHeater.hold 10:00,78.1C @18:09:49.41 Lysis complete. Syringe pressure @27.3psi @18:09:49.44 Archive syringe pumping up to 5 strokes to reach 40psi @18:09:49.45 Archive stroke #1 of 5 @18:09:49.54 Archive.in :pressurize @18:09:49.60 Archive.seek :pressurized @18:10:02.13 27.0psi after pressurize @18:10:07.73 26.8psi after pressurize (stable) @18:10:07.75 Archive.in :compress @18:10:07.81 Archive.seek :empty @18:10:15.82 Reached 40.6 of 40.0psi after pumping 1587uL (0.92 strokes) @18:10:15.85 Archive.in :default @18:10:15.91 Archive.hold @18:10:15.98 delay 2 seconds @18:10:18.01 Archive.in :fast @18:10:18.07 Archive.seek :full @18:10:24.75 Lysis1.seek 138uL @18:10:38.63 delay 1 second @18:10:39.68 Archive.seek :full @18:10:39.71 Lysis1.seek :empty @18:10:53.68 Handoff.select :blocked @18:10:53.79 Initial air pressure @48.7psi @18:10:53.87 Lysis1.in :extract @18:10:53.97 Lysis1.seek 600uL,nil @18:10:57.01 Archive syringe pumping up to 6 strokes to reach 25psi @18:10:57.02 Archive stroke #1 of 6 @18:10:57.18 Archive.in :pressurize @18:10:57.27 Archive.seek :pressurized @18:11:09.82 10.8psi after pressurize @18:11:36.92 16.0psi after pressurize (stable) @18:11:36.97 Archive.in :compress @18:11:37.06 Archive.seek :empty @18:11:45.74 Archive.in :default @18:11:45.82 Archive.hold @18:11:46.02 Initial 600uL lysate @17.2psi @18:11:46.04 Handoff.select :blocked @18:11:46.13 Initial air pressure @17.5psi @18:11:46.22 Lysis1.seek 825uL,nil @18:11:48.03 Archive syringe pumping up to 6 strokes to reach 17psi @18:11:48.05 Archive stroke #1 of 6 @18:11:48.20 Archive.in :compress @18:11:48.26 Archive.seek :empty @18:11:48.70 Archive.in :default @18:11:48.78 Archive.hold @18:11:49.04 Recovered 825uL lysate @14.2psi @18:11:49.07 Air.in :halfSpeed @18:11:49.13 Air.seek :fullVent @18:11:51.35 Puck Top Vented, bottom @2.9psi @18:11:51.36 Handoff.select :waste @18:11:51.48 delay 5 seconds @18:11:56.56 Handoff open to waste, bottom @0.3psi @18:11:56.58 Air.in :default @18:11:56.64 Air.seek :evac @18:11:58.07 Lysis1.in :DAmix @18:11:58.19 Lysis2.in :DAmix @18:11:58.77 Lysis1.seek 575uL @18:11:58.83 Lysis2.seek 1480uL @18:12:33.92 delay 5 seconds @18:12:38.94 Handoff.select SPR @18:12:39.07 SPR.command "start 2 14" @18:12:39.88 SPR stabilized @18:12:40.03 delay 10 seconds @18:12:50.05 SPR.command "start 1 0" @18:12:53.24 Waiting up to 4:00 for SPR to enter state 1 @18:14:57.50 SPR.command "hold_on" @18:14:58.92 SPR Dilution 1 of 1 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:14:59.54 Lysis1.seek 275uL @18:14:59.60 Lysis2.seek 1180uL @18:15:41.69 delay 15 seconds @18:15:56.71 SPR.command "hold_off" @18:16:00.06 Waiting up to 38:00 for SPR to enter state 7 @18:33:55.37 SPRsummary:-2.47867,106.917,129.443,186.919 after 3045s @18:40:32.60 SPR analysis completed @18:40:38.76 Ended log spr23mar2417h.spr.bz2 @18:40:38.78 SPR.command "stats_off" @18:40:39.05 Handoff.select :close @18:40:39.18
Gate.power :heat,:OFF @18:40:39.20 Gate.power :cartridge,:OFF @18:40:39.28 PinPort.in :unlocking @18:40:39.34 PinPort.seek :clear @18:40:39.81 Carousel.in :default @18:40:39.94 Carousel.home.seek 56 @18:40:40.27 Plunge.seek :locked @18:40:41.16 Archive.in :unlocking @18:40:41.30 Archive.seek :clear @18:40:41.38 Air.in :unlocking @18:40:41.55 Air.seek :clear @18:40:41.65 Lysis1.in :unlocking @18:40:41.84 Lysis1.seek :clear @18:40:41.92 Lysis2.in :unlocking @18:40:42.02 Lysis2.seek :clear @18:40:46.50
Gate.power :motor,:OFF @18:40:46.52 Gate.power :core,:OFF @18:40:46.53 Gate.power :analytic1,:OFF @18:40:46.54 Gate.power :analytic2,:OFF @18:40:46.56 Gate.power :analytic3,:OFF @18:40:46.57 Gate.power :main,:OFF @18:40:46.58 Safely stopped and ready to power off @18:40:47.48 Cartridge::None in MAIN -- Not enough dry cartridges remain @17:31:57.55PDT26-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:31:57.58 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:32:01.95 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:32:02.66 Sea pressure (Abs) tare is 6% of full scale @17:32:06.53 Polling Can every 2:00 @17:32:06.57 Loading /home/methane/esp2/bin/processAll mission @17:32:06.91 Sea pressure (Abs) tare is 6% of full scale @17:32:06.93 Sampling default goal volumes @17:32:06.94 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @17:32:07.11 Gate.power :analytic1,:ON,limit to 5000mA @17:32:07.12 Gate.power :analytic2,:ON,limit to 5000mA @17:32:07.14 Gate.power :analytic3,:ON,limit to 5000mA @17:32:07.84 Archive.configure :default @17:32:08.00 Air.configure :default @17:32:08.14 Lysis1.configure :default @17:32:08.21 SPR Module 1, FW Rev. B, FPGA Rev. C @17:32:08.31
Lysis2.configure :default @17:32:08.46 Plunge.configure :default @17:32:08.65 SamplePump.configure :default @17:32:08.80 Toroid.configure :default @17:32:08.87 Toroid.hold @17:32:08.94 Toroid.configure :default @17:32:09.09 Carousel.configure :default @17:32:09.15 Carousel.hold @17:32:09.22 Carousel.configure :default @17:32:09.37 Pressure.configure :default @17:32:09.51 ExtraPump.configure :default @17:32:09.64 PinPort.configure :default @17:32:09.70 All elf controllers running firmware version 4.83 @17:32:10.49 Toroid.seek :clear @17:32:10.57 Carousel.in :default @17:32:10.73 Carousel.home.seek 60 @17:32:10.99 Selecting Cartridge 56 @17:32:11.30 Carousel.seek 56 @17:32:13.44 Lysis1.in :fast @17:32:13.50 Lysis1.seek :locked @17:32:16.13 Plunge.seek :locked @17:32:16.28 Air.in :fast @17:32:16.34 Air.seek :locked @17:32:17.93
Carousel.reconfigure :locked @17:32:18.08 Powering cartridge bus @17:32:20.73 Gate.setIR 1750,5000,0,6 @17:32:20.85 Gate.setIR 0,5000,0,6 @17:32:20.92 PuckHeater.configure :default @17:32:20.98 Gate.power :heat,:ON,limit to 5000mA @17:32:21.77 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @17:32:21.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:32:23.36 SPR.command "read_sequences" @17:32:24.17 SPR.command "stats_on" @17:32:24.33 SPR.command "start 0 1" @17:32:25.03 SPR.start /var/log/methane/spr23mar2617h.spr.bz2 @17:32:27.02 Waiting for baseline SPR stabilization @17:32:27.04 Handoff.select :waste @17:32:33.75 Syringe tare is 16% of full scale @17:32:33.78 PinPort.in :locking @17:32:33.84 PinPort.seek :locked @17:32:34.33 Archive.in :fast @17:32:34.39 Lysis2.in :fast @17:32:34.45 Lysis1.seek 1000uL @17:32:34.67 Archive.seek :full @17:32:34.78 Lysis2.seek 1000uL @17:32:39.25 delayUntil "17:42:26.56" @17:42:26.57 Lysis1.in :DAmix @17:42:26.73 Lysis2.in :DAmix @17:42:27.27 Lysis1.seek 750uL @17:42:27.33 Lysis2.seek 750uL @17:43:02.47 delay 5 seconds @17:43:07.49 Handoff.select SPR @17:43:07.61 SPR.command "start 2 14" @17:43:08.29 SPR stabilized @17:43:08.44 delay 10 seconds @17:43:18.46 SPR.command "start 1 0" @17:43:21.66 Waiting up to 4:00 for SPR to enter state 1 @17:45:25.92 SPR.command "hold_on" @17:45:27.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:45:27.92 Lysis1.seek 450uL @17:45:27.97 Lysis2.seek 450uL @17:46:10.05 delay 15 seconds @17:46:25.07 SPR.command "hold_off" @17:46:27.99 Waiting up to 38:00 for SPR to enter state 7 @18:04:27.60 SPRsummary:-60.8893,125.552,129.292,223.565 after 2005s @18:11:04.96 delay 10 seconds @18:11:14.98 SPR.command "start 1 0" @18:11:17.68 Waiting up to 4:00 for SPR to enter state 1 @18:13:21.96 SPR.command "hold_on" @18:13:23.36 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:23.93 Lysis1.seek 300uL @18:13:23.99 Lysis2.seek 300uL @18:13:45.11 delay 15 seconds @18:14:00.13 SPR.command "hold_off" @18:14:03.20 Waiting up to 38:00 for SPR to enter state 7 @18:32:58.79 Session Begun @18:33:02.64 -> showlog -50 @17:32:20.92 PuckHeater.configure :default @17:32:20.98 Gate.power :heat,:ON,limit to 5000mA @17:32:21.77 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @17:32:21.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:32:23.36 SPR.command "read_sequences" @17:32:24.17 SPR.command "stats_on" @17:32:24.33 SPR.command "start 0 1" @17:32:25.03 SPR.start /var/log/methane/spr23mar2617h.spr.bz2 @17:32:27.02 Waiting for baseline SPR stabilization @17:32:27.04 Handoff.select :waste @17:32:33.75 Syringe tare is 16% of full scale @17:32:33.78 PinPort.in :locking @17:32:33.84 PinPort.seek :locked @17:32:34.33 Archive.in :fast @17:32:34.39 Lysis2.in :fast @17:32:34.45 Lysis1.seek 1000uL @17:32:34.67 Archive.seek :full @17:32:34.78 Lysis2.seek 1000uL @17:32:39.25 delayUntil "17:42:26.56" @17:42:26.57 Lysis1.in :DAmix @17:42:26.73 Lysis2.in :DAmix @17:42:27.27 Lysis1.seek 750uL @17:42:27.33 Lysis2.seek 750uL @17:43:02.47 delay 5 seconds @17:43:07.49 Handoff.select SPR @17:43:07.61 SPR.command "start 2 14" @17:43:08.29 SPR stabilized @17:43:08.44 delay 10 seconds @17:43:18.46 SPR.command "start 1 0" @17:43:21.66 Waiting up to 4:00 for SPR to enter state 1 @17:45:25.92 SPR.command "hold_on" @17:45:27.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:45:27.92 Lysis1.seek 450uL @17:45:27.97 Lysis2.seek 450uL @17:46:10.05 delay 15 seconds @17:46:25.07 SPR.command "hold_off" @17:46:27.99 Waiting up to 38:00 for SPR to enter state 7 @18:04:27.60 SPRsummary:-60.8893,125.552,129.292,223.565 after 2005s @18:11:04.96 delay 10 seconds @18:11:14.98 SPR.command "start 1 0" @18:11:17.68 Waiting up to 4:00 for SPR to enter state 1 @18:13:21.96 SPR.command "hold_on" @18:13:23.36 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:23.93 Lysis1.seek 300uL @18:13:23.99 Lysis2.seek 300uL @18:13:45.11 delay 15 seconds @18:14:00.13 SPR.command "hold_off" @18:14:03.20 Waiting up to 38:00 for SPR to enter state 7 @18:32:58.79 Session Begun @18:33:02.64 -> showlog -50 @18:38:40.18 delay 10 seconds @18:38:50.20 SPR.command "start 1 0" @18:38:53.41 Waiting up to 4:00 for SPR to enter state 1 @18:40:58.13 SPR.command "hold_on" @18:40:59.51 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:41:00.10 Lysis1.seek 150uL @18:41:00.18 Lysis2.seek 150uL @18:41:21.31 delay 15 seconds @18:41:36.33 SPR.command "hold_off" @18:41:39.35 Waiting up to 38:00 for SPR to enter state 7 @18:56:59.59 Session Ended @19:06:16.33 delay 10 seconds @19:06:26.35 SPR.command "start 1 0" @19:06:29.56 Waiting up to 4:00 for SPR to enter state 1 @19:08:34.28 SPR.command "hold_on" @19:08:35.66 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:08:36.20 Lysis1.seek 0uL @19:08:36.26 Lysis2.seek 0uL @19:08:57.38 delay 15 seconds @19:09:12.40 SPR.command "hold_off" @19:09:15.51 Waiting up to 38:00 for SPR to enter state 7 @19:33:52.34 SPR analysis completed @19:33:54.40 Ended log spr23mar2617h.spr.bz2 @19:33:54.42 SPR.command "stats_off" @19:33:55.24 Intake.close @19:33:55.31 Exhaust.close @19:33:55.39 Bypass.close @19:33:55.49 Handoff.select :close @19:33:55.68
Gate.power :heat,:OFF @19:33:55.69 Gate.power :cartridge,:OFF @19:33:55.77 PinPort.in :unlocking @19:33:55.84 PinPort.seek :clear @19:33:56.30 Carousel.in :default @19:33:56.44 Carousel.home.seek 56 @19:33:56.79 Plunge.seek :locked @19:33:56.97 Archive.in :unlocking @19:33:57.12 Archive.seek :clear @19:33:57.20 Air.in :unlocking @19:33:57.38 Air.seek :clear @19:33:57.48 Lysis1.in :unlocking @19:33:57.63 Lysis1.seek :clear @19:33:57.69 Lysis2.in :unlocking @19:33:57.81 Lysis2.seek :clear @19:34:02.96
Gate.power :motor,:OFF @19:34:02.98 Gate.power :core,:OFF @19:34:02.99 Gate.power :analytic1,:OFF @19:34:03.00 Gate.power :analytic2,:OFF @19:34:03.02 Gate.power :analytic3,:OFF @19:34:03.03 Gate.power :main,:OFF @19:34:03.04 Safely stopped and ready to power off @19:34:06.80 Gate.power :main,:ON,limit to 5000mA @19:34:06.82 Gate.power :analytic1,:ON,limit to 5000mA @19:34:06.83 Gate.power :analytic2,:ON,limit to 5000mA @19:34:06.85 Gate.power :analytic3,:ON,limit to 5000mA @19:34:06.93 Powering core bus @19:34:09.28 SPR Module 1, FW Rev. B, FPGA Rev. C @19:34:09.69
Archive.configure :default @19:34:09.78 Air.configure :default @19:34:09.84 Lysis1.configure :default @19:34:09.91 Lysis2.configure :default @19:34:09.99 Plunge.configure :default @19:34:10.09 SamplePump.configure :default @19:34:10.18 Toroid.configure :default @19:34:10.27 Carousel.configure :default @19:34:10.35 Pressure.configure :default @19:34:10.44 ExtraPump.configure :default @19:34:10.51 PinPort.configure :default @19:34:10.57 All elf controllers running firmware version 4.83 @19:34:10.60 Gate.power :motor,:ON,limit to 5000mA @19:34:11.02 Archive.home.jog 0 @19:34:11.61 Carousel.home.jog 0 @19:34:11.66 Toroid.home.jog 0 @19:34:11.69 Lysis2.home.jog 0 @19:34:11.72 Lysis1.home.jog 0 @19:34:11.75 PinPort.home.jog 0 @19:34:11.77 Plunge.home.jog 0 @19:34:11.80 Air.home.jog 0 @19:34:13.05 Archive.in :homing @19:34:13.44 Archive.jog 1377 @19:34:13.48 Toroid.in :homing @19:34:13.51 Plunge.in :homing @19:34:13.55 Lysis1.in :homing @19:34:13.60 PinPort.in :homing @19:34:13.64 Lysis2.in :homing @19:34:13.88 Toroid.jog 619 @19:34:13.98 Lysis1.jog 1380 @19:34:14.02 Plunge.jog 1520 @19:34:14.07 Lysis2.jog 1387 @19:34:14.12 PinPort.jog -108 @19:34:14.40 Air.in :homing @19:34:14.46 Air.jog 1379 @19:34:14.54 PinPort.setRawPosition! 0 @19:34:14.64 PinPort.seek :home @19:34:14.73 PinPort.in :default @19:34:14.89 Archive.setRawPosition! 50 @19:34:14.94 Archive.seek :home @19:34:15.21 Archive.in :default @19:34:15.31 Lysis1.setRawPosition! 50 @19:34:15.38 Lysis2.setRawPosition! 50 @19:34:15.42 Lysis1.seek :home @19:34:15.51 Lysis2.seek :home @19:34:15.61 Plunge.setRawPosition! 50 @19:34:15.66 Plunge.seek :home @19:34:15.76 Air.setRawPosition! 50 @19:34:15.79 Lysis1.in :default @19:34:15.88 Air.seek :home @19:34:16.01 Lysis2.in :default @19:34:16.04 Plunge.in :default @19:34:16.20 Toroid.setRawPosition! 0 @19:34:16.23 Air.in :default @19:34:16.32 Toroid.seek :home @19:34:16.44 Toroid.in :default @19:34:16.64
Toroid.seek :clear @19:34:18.25 Carousel.in :default @19:34:18.37 Carousel.home.seek 56 @19:34:18.66 Selecting Cartridge 55 @19:34:18.95 Carousel.seek 55 @19:34:20.01 Lysis1.in :fast @19:34:20.07 Lysis1.seek :locked @19:34:22.72 Plunge.seek :locked @19:34:22.88 Air.in :fast @19:34:23.41 Air.seek :locked @19:34:24.99
Carousel.reconfigure :locked @19:34:25.15 Powering cartridge bus @19:34:27.79 Gate.setIR 1750,5000,0,6 @19:34:27.91 Gate.setIR 0,5000,0,6 @19:34:27.98 PuckHeater.configure :default @19:34:28.04 Gate.power :heat,:ON,limit to 5000mA @19:34:28.78 Mounted lysis Cartridge board#Q05048 @19:34:28.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:34:30.36 SPR.command "read_sequences" @19:34:30.89 SPR.command "stats_on" @19:34:31.05 SPR.command "start 0 1" @19:34:31.75 SPR.start /var/log/methane/spr23mar2619h.spr.bz2 @19:34:33.74 Waiting for baseline SPR stabilization @19:34:33.76 Handoff.select :waste @19:34:40.45 Syringe tare is 16% of full scale @19:34:40.48 PinPort.in :locking @19:34:40.54 PinPort.seek :locked @19:34:41.03 Archive.in :fast @19:34:41.14 Lysis2.in :fast @19:34:41.18 Lysis1.seek 1000uL @19:34:41.22 Archive.seek :full @19:34:41.38 Lysis2.seek 1000uL @19:34:45.93 delayUntil "19:44:33.31" @19:44:33.32 Lysis1.in :DAmix @19:44:33.45 Lysis2.in :DAmix @19:44:33.99 Lysis1.seek 750uL @19:44:34.04 Lysis2.seek 750uL @19:45:09.14 delay 5 seconds @19:45:14.16 Handoff.select SPR @19:45:14.29 SPR.command "start 2 14" @19:45:15.02 SPR stabilized @19:45:15.17 delay 10 seconds @19:45:25.19 SPR.command "start 1 0" @19:45:28.38 Waiting up to 4:00 for SPR to enter state 1 @19:47:32.68 SPR.command "hold_on" @19:47:34.06 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @19:47:34.67 Lysis1.seek 450uL @19:47:34.73 Lysis2.seek 450uL @19:48:16.80 delay 15 seconds @19:48:31.82 SPR.command "hold_off" @19:48:34.71 Waiting up to 38:00 for SPR to enter state 7 @20:06:34.32 SPRsummary:-8.30667,170.013,166.901,268.813 after 2005s @20:13:11.69 delay 10 seconds @20:13:21.71 SPR.command "start 1 0" @20:13:24.92 Waiting up to 4:00 for SPR to enter state 1 @20:15:29.64 SPR.command "hold_on" @20:15:31.55 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:15:32.13 Lysis1.seek 300uL @20:15:32.18 Lysis2.seek 300uL @20:15:53.28 delay 15 seconds @20:16:08.32 SPR.command "hold_off" @20:16:10.87 Waiting up to 38:00 for SPR to enter state 7 @20:40:46.90 delay 10 seconds @20:40:56.92 SPR.command "start 1 0" @20:41:00.13 Waiting up to 4:00 for SPR to enter state 1 @20:43:04.85 SPR.command "hold_on" @20:43:06.23 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:43:06.83 Lysis1.seek 150uL @20:43:06.88 Lysis2.seek 150uL @20:43:27.98 delay 15 seconds @20:43:43.00 SPR.command "hold_off" @20:43:46.08 Waiting up to 38:00 for SPR to enter state 7 @21:08:23.06 delay 10 seconds @21:08:33.08 SPR.command "start 1 0" @21:08:35.77 Waiting up to 4:00 for SPR to enter state 1 @21:10:40.06 SPR.command "hold_on" @21:10:41.44 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @21:10:42.01 Lysis1.seek 0uL @21:10:42.07 Lysis2.seek 0uL @21:11:03.17 delay 15 seconds @21:11:18.19 SPR.command "hold_off" @21:11:21.29 Waiting up to 38:00 for SPR to enter state 7 @21:35:58.13 SPR analysis completed @21:36:00.24 Ended log spr23mar2619h.spr.bz2 @21:36:00.26 SPR.command "stats_off" @21:36:00.65 Handoff.select :close @21:36:00.78
Gate.power :heat,:OFF @21:36:00.79 Gate.power :cartridge,:OFF @21:36:00.88 PinPort.in :unlocking @21:36:00.94 PinPort.seek :clear @21:36:01.41 Carousel.in :default @21:36:01.53 Carousel.home.seek 55 @21:36:01.87 Plunge.seek :locked @21:36:02.04 Archive.in :unlocking @21:36:02.19 Archive.seek :clear @21:36:02.32 Air.in :unlocking @21:36:02.45 Air.seek :clear @21:36:02.53 Lysis1.in :unlocking @21:36:02.74 Lysis1.seek :clear @21:36:02.79 Lysis2.in :unlocking @21:36:02.93 Lysis2.seek :clear @21:36:08.04
Gate.power :motor,:OFF @21:36:08.06 Gate.power :core,:OFF @21:36:08.07 Gate.power :analytic1,:OFF @21:36:08.08 Gate.power :analytic2,:OFF @21:36:08.10 Gate.power :analytic3,:OFF @21:36:08.11 Gate.power :main,:OFF @21:36:08.12 Safely stopped and ready to power off @21:36:11.88 Gate.power :main,:ON,limit to 5000mA @21:36:11.90 Gate.power :analytic1,:ON,limit to 5000mA @21:36:11.91 Gate.power :analytic2,:ON,limit to 5000mA @21:36:11.93 Gate.power :analytic3,:ON,limit to 5000mA @21:36:12.04 Powering core bus @21:36:14.35 SPR Module 1, FW Rev. B, FPGA Rev. C @21:36:14.82
Archive.configure :default @21:36:14.91 Air.configure :default @21:36:14.97 Lysis1.configure :default @21:36:15.04 Lysis2.configure :default @21:36:15.13 Plunge.configure :default @21:36:15.21 SamplePump.configure :default @21:36:15.31 Toroid.configure :default @21:36:15.40 Carousel.configure :default @21:36:15.48 Pressure.configure :default @21:36:15.58 ExtraPump.configure :default @21:36:15.64 PinPort.configure :default @21:36:15.70 All elf controllers running firmware version 4.83 @21:36:15.72 Gate.power :motor,:ON,limit to 5000mA @21:36:16.01 Archive.home.jog 0 @21:36:17.20 PinPort.home.jog 0 @21:36:17.25 Carousel.home.jog 0 @21:36:17.31 Toroid.home.jog 0 @21:36:17.35 Lysis2.home.jog 0 @21:36:17.37 Lysis1.home.jog 0 @21:36:17.40 Air.home.jog 0 @21:36:17.43 Plunge.home.jog 0 @21:36:18.26 Archive.in :homing @21:36:18.48 Archive.jog 1375 @21:36:18.55 PinPort.in :homing @21:36:18.58 Plunge.in :homing @21:36:18.63 Lysis1.in :homing @21:36:18.70 Toroid.in :homing @21:36:18.74 Air.in :homing @21:36:18.81 Lysis2.in :homing @21:36:19.03 Plunge.jog 1526 @21:36:19.08 PinPort.jog -109 @21:36:19.14 Lysis1.jog 1379 @21:36:19.18 Toroid.jog 627 @21:36:19.21 Air.jog 1381 @21:36:19.28 Lysis2.jog 1391 @21:36:19.75 PinPort.setRawPosition! 0 @21:36:19.80 PinPort.seek :home @21:36:19.89 PinPort.in :default @21:36:19.97 Archive.setRawPosition! 50 @21:36:20.03 Archive.seek :home @21:36:20.31 Archive.in :default @21:36:20.54 Plunge.setRawPosition! 50 @21:36:20.61 Plunge.seek :home @21:36:20.70 Lysis1.setRawPosition! 50 @21:36:20.78 Lysis1.seek :home @21:36:20.88 Lysis2.setRawPosition! 50 @21:36:20.91 Air.setRawPosition! 50 @21:36:20.98 Lysis2.seek :home @21:36:21.02 Air.seek :home @21:36:21.19 Lysis1.in :default @21:36:21.22 Plunge.in :default @21:36:21.39 Lysis2.in :default @21:36:21.42 Air.in :default @21:36:21.69 Toroid.setRawPosition! 0 @21:36:21.74 Toroid.seek :home @21:36:21.83 Toroid.in :default @21:36:21.91
Toroid.seek :clear @21:36:23.52 Carousel.in :default @21:36:23.65 Carousel.home.seek 55 @21:36:23.91 Selecting Cartridge 54 @21:36:24.20 Carousel.seek 54 @21:36:25.66 Lysis1.in :fast @21:36:25.81 Lysis1.seek :locked @21:36:28.44 Plunge.seek :locked @21:36:28.64 Air.in :fast @21:36:28.70 Air.seek :locked @21:36:30.28
Carousel.reconfigure :locked @21:36:30.43 Powering cartridge bus @21:36:33.07 Gate.setIR 1750,5000,0,6 @21:36:33.19 Gate.setIR 0,5000,0,6 @21:36:33.26 PuckHeater.configure :default @21:36:33.32 Gate.power :heat,:ON,limit to 5000mA @21:36:34.22 Mounted lysis Cartridge board#Q05045 @21:36:34.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:36:35.83 SPR.command "read_sequences" @21:36:35.97 SPR.command "stats_on" @21:36:36.13 SPR.command "start 0 1" @21:36:36.83 SPR.start /var/log/methane/spr23mar2621h.spr.bz2 @21:36:38.82 Waiting for baseline SPR stabilization @21:36:38.85 Handoff.select :waste @21:36:45.54 Syringe tare is 16% of full scale @21:36:45.56 PinPort.in :locking @21:36:45.62 PinPort.seek :locked @21:36:46.11 Archive.in :fast @21:36:46.22 Lysis2.in :fast @21:36:46.28 Lysis1.seek 1000uL @21:36:46.32 Archive.seek :full @21:36:46.43 Lysis2.seek 1000uL @21:36:51.00 delayUntil "21:46:38.39" @21:46:38.40 Lysis1.in :DAmix @21:46:38.53 Lysis2.in :DAmix @21:46:39.13 Lysis1.seek 750uL @21:46:39.21 Lysis2.seek 750uL @21:47:14.27 delay 5 seconds @21:47:19.29 Handoff.select SPR @21:47:19.42 SPR.command "start 2 14" @21:47:20.10 SPR stabilized @21:47:20.25 delay 10 seconds @21:47:30.27 SPR.command "start 1 0" @21:47:33.46 Waiting up to 4:00 for SPR to enter state 1 @21:49:37.76 SPR.command "hold_on" @21:49:39.14 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:49:39.73 Lysis1.seek 450uL @21:49:39.79 Lysis2.seek 450uL @21:50:21.85 delay 15 seconds @21:50:36.87 SPR.command "hold_off" @21:50:39.79 Waiting up to 38:00 for SPR to enter state 7 @22:08:39.40 SPRsummary:-4.59333,178.603,170.319,274.397 after 2005s @22:15:16.79 delay 10 seconds @22:15:26.81 SPR.command "start 1 0" @22:15:30.02 Waiting up to 4:00 for SPR to enter state 1 @22:16:24.97 Session Begun @22:16:30.47 -> showlog -100 @21:36:16.01 Archive.home.jog 0 @21:36:17.20 PinPort.home.jog 0 @21:36:17.25 Carousel.home.jog 0 @21:36:17.31 Toroid.home.jog 0 @21:36:17.35 Lysis2.home.jog 0 @21:36:17.37 Lysis1.home.jog 0 @21:36:17.40 Air.home.jog 0 @21:36:17.43 Plunge.home.jog 0 @21:36:18.26 Archive.in :homing @21:36:18.48 Archive.jog 1375 @21:36:18.55 PinPort.in :homing @21:36:18.58 Plunge.in :homing @21:36:18.63 Lysis1.in :homing @21:36:18.70 Toroid.in :homing @21:36:18.74 Air.in :homing @21:36:18.81 Lysis2.in :homing @21:36:19.03 Plunge.jog 1526 @21:36:19.08 PinPort.jog -109 @21:36:19.14 Lysis1.jog 1379 @21:36:19.18 Toroid.jog 627 @21:36:19.21 Air.jog 1381 @21:36:19.28 Lysis2.jog 1391 @21:36:19.75 PinPort.setRawPosition! 0 @21:36:19.80 PinPort.seek :home @21:36:19.89 PinPort.in :default @21:36:19.97 Archive.setRawPosition! 50 @21:36:20.03 Archive.seek :home @21:36:20.31 Archive.in :default @21:36:20.54 Plunge.setRawPosition! 50 @21:36:20.61 Plunge.seek :home @21:36:20.70 Lysis1.setRawPosition! 50 @21:36:20.78 Lysis1.seek :home @21:36:20.88 Lysis2.setRawPosition! 50 @21:36:20.91 Air.setRawPosition! 50 @21:36:20.98 Lysis2.seek :home @21:36:21.02 Air.seek :home @21:36:21.19 Lysis1.in :default @21:36:21.22 Plunge.in :default @21:36:21.39 Lysis2.in :default @21:36:21.42 Air.in :default @21:36:21.69 Toroid.setRawPosition! 0 @21:36:21.74 Toroid.seek :home @21:36:21.83 Toroid.in :default @21:36:21.91
Toroid.seek :clear @21:36:23.52 Carousel.in :default @21:36:23.65 Carousel.home.seek 55 @21:36:23.91 Selecting Cartridge 54 @21:36:24.20 Carousel.seek 54 @21:36:25.66 Lysis1.in :fast @21:36:25.81 Lysis1.seek :locked @21:36:28.44 Plunge.seek :locked @21:36:28.64 Air.in :fast @21:36:28.70 Air.seek :locked @21:36:30.28
Carousel.reconfigure :locked @21:36:30.43 Powering cartridge bus @21:36:33.07 Gate.setIR 1750,5000,0,6 @21:36:33.19 Gate.setIR 0,5000,0,6 @21:36:33.26 PuckHeater.configure :default @21:36:33.32 Gate.power :heat,:ON,limit to 5000mA @21:36:34.22 Mounted lysis Cartridge board#Q05045 @21:36:34.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:36:35.83 SPR.command "read_sequences" @21:36:35.97 SPR.command "stats_on" @21:36:36.13 SPR.command "start 0 1" @21:36:36.83 SPR.start /var/log/methane/spr23mar2621h.spr.bz2 @21:36:38.82 Waiting for baseline SPR stabilization @21:36:38.85 Handoff.select :waste @21:36:45.54 Syringe tare is 16% of full scale @21:36:45.56 PinPort.in :locking @21:36:45.62 PinPort.seek :locked @21:36:46.11 Archive.in :fast @21:36:46.22 Lysis2.in :fast @21:36:46.28 Lysis1.seek 1000uL @21:36:46.32 Archive.seek :full @21:36:46.43 Lysis2.seek 1000uL @21:36:51.00 delayUntil "21:46:38.39" @21:46:38.40 Lysis1.in :DAmix @21:46:38.53 Lysis2.in :DAmix @21:46:39.13 Lysis1.seek 750uL @21:46:39.21 Lysis2.seek 750uL @21:47:14.27 delay 5 seconds @21:47:19.29 Handoff.select SPR @21:47:19.42 SPR.command "start 2 14" @21:47:20.10 SPR stabilized @21:47:20.25 delay 10 seconds @21:47:30.27 SPR.command "start 1 0" @21:47:33.46 Waiting up to 4:00 for SPR to enter state 1 @21:49:37.76 SPR.command "hold_on" @21:49:39.14 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:49:39.73 Lysis1.seek 450uL @21:49:39.79 Lysis2.seek 450uL @21:50:21.85 delay 15 seconds @21:50:36.87 SPR.command "hold_off" @21:50:39.79 Waiting up to 38:00 for SPR to enter state 7 @22:08:39.40 SPRsummary:-4.59333,178.603,170.319,274.397 after 2005s @22:15:16.79 delay 10 seconds @22:15:26.81 SPR.command "start 1 0" @22:15:30.02 Waiting up to 4:00 for SPR to enter state 1 @22:16:24.97 Session Begun @22:16:30.47 -> showlog -100 @22:17:07.09 -> showlog -500 @17:31:57.55PDT26-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:31:57.58 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:32:01.95 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:32:02.66 Sea pressure (Abs) tare is 6% of full scale @17:32:06.53 Polling Can every 2:00 @17:32:06.57 Loading /home/methane/esp2/bin/processAll mission @17:32:06.91 Sea pressure (Abs) tare is 6% of full scale @17:32:06.93 Sampling default goal volumes @17:32:06.94 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @17:32:07.11 Gate.power :analytic1,:ON,limit to 5000mA @17:32:07.12 Gate.power :analytic2,:ON,limit to 5000mA @17:32:07.14 Gate.power :analytic3,:ON,limit to 5000mA @17:32:07.84 Archive.configure :default @17:32:08.00 Air.configure :default @17:32:08.14 Lysis1.configure :default @17:32:08.21 SPR Module 1, FW Rev. B, FPGA Rev. C @17:32:08.31
Lysis2.configure :default @17:32:08.46 Plunge.configure :default @17:32:08.65 SamplePump.configure :default @17:32:08.80 Toroid.configure :default @17:32:08.87 Toroid.hold @17:32:08.94 Toroid.configure :default @17:32:09.09 Carousel.configure :default @17:32:09.15 Carousel.hold @17:32:09.22 Carousel.configure :default @17:32:09.37 Pressure.configure :default @17:32:09.51 ExtraPump.configure :default @17:32:09.64 PinPort.configure :default @17:32:09.70 All elf controllers running firmware version 4.83 @17:32:10.49 Toroid.seek :clear @17:32:10.57 Carousel.in :default @17:32:10.73 Carousel.home.seek 60 @17:32:10.99 Selecting Cartridge 56 @17:32:11.30 Carousel.seek 56 @17:32:13.44 Lysis1.in :fast @17:32:13.50 Lysis1.seek :locked @17:32:16.13 Plunge.seek :locked @17:32:16.28 Air.in :fast @17:32:16.34 Air.seek :locked @17:32:17.93
Carousel.reconfigure :locked @17:32:18.08 Powering cartridge bus @17:32:20.73 Gate.setIR 1750,5000,0,6 @17:32:20.85 Gate.setIR 0,5000,0,6 @17:32:20.92 PuckHeater.configure :default @17:32:20.98 Gate.power :heat,:ON,limit to 5000mA @17:32:21.77 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @17:32:21.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:32:23.36 SPR.command "read_sequences" @17:32:24.17 SPR.command "stats_on" @17:32:24.33 SPR.command "start 0 1" @17:32:25.03 SPR.start /var/log/methane/spr23mar2617h.spr.bz2 @17:32:27.02 Waiting for baseline SPR stabilization @17:32:27.04 Handoff.select :waste @17:32:33.75 Syringe tare is 16% of full scale @17:32:33.78 PinPort.in :locking @17:32:33.84 PinPort.seek :locked @17:32:34.33 Archive.in :fast @17:32:34.39 Lysis2.in :fast @17:32:34.45 Lysis1.seek 1000uL @17:32:34.67 Archive.seek :full @17:32:34.78 Lysis2.seek 1000uL @17:32:39.25 delayUntil "17:42:26.56" @17:42:26.57 Lysis1.in :DAmix @17:42:26.73 Lysis2.in :DAmix @17:42:27.27 Lysis1.seek 750uL @17:42:27.33 Lysis2.seek 750uL @17:43:02.47 delay 5 seconds @17:43:07.49 Handoff.select SPR @17:43:07.61 SPR.command "start 2 14" @17:43:08.29 SPR stabilized @17:43:08.44 delay 10 seconds @17:43:18.46 SPR.command "start 1 0" @17:43:21.66 Waiting up to 4:00 for SPR to enter state 1 @17:45:25.92 SPR.command "hold_on" @17:45:27.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:45:27.92 Lysis1.seek 450uL @17:45:27.97 Lysis2.seek 450uL @17:46:10.05 delay 15 seconds @17:46:25.07 SPR.command "hold_off" @17:46:27.99 Waiting up to 38:00 for SPR to enter state 7 @18:04:27.60 SPRsummary:-60.8893,125.552,129.292,223.565 after 2005s @18:11:04.96 delay 10 seconds @18:11:14.98 SPR.command "start 1 0" @18:11:17.68 Waiting up to 4:00 for SPR to enter state 1 @18:13:21.96 SPR.command "hold_on" @18:13:23.36 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:23.93 Lysis1.seek 300uL @18:13:23.99 Lysis2.seek 300uL @18:13:45.11 delay 15 seconds @18:14:00.13 SPR.command "hold_off" @18:14:03.20 Waiting up to 38:00 for SPR to enter state 7 @18:32:58.79 Session Begun @18:33:02.64 -> showlog -50 @17:32:20.92 PuckHeater.configure :default @17:32:20.98 Gate.power :heat,:ON,limit to 5000mA @17:32:21.77 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @17:32:21.80 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:32:23.36 SPR.command "read_sequences" @17:32:24.17 SPR.command "stats_on" @17:32:24.33 SPR.command "start 0 1" @17:32:25.03 SPR.start /var/log/methane/spr23mar2617h.spr.bz2 @17:32:27.02 Waiting for baseline SPR stabilization @17:32:27.04 Handoff.select :waste @17:32:33.75 Syringe tare is 16% of full scale @17:32:33.78 PinPort.in :locking @17:32:33.84 PinPort.seek :locked @17:32:34.33 Archive.in :fast @17:32:34.39 Lysis2.in :fast @17:32:34.45 Lysis1.seek 1000uL @17:32:34.67 Archive.seek :full @17:32:34.78 Lysis2.seek 1000uL @17:32:39.25 delayUntil "17:42:26.56" @17:42:26.57 Lysis1.in :DAmix @17:42:26.73 Lysis2.in :DAmix @17:42:27.27 Lysis1.seek 750uL @17:42:27.33 Lysis2.seek 750uL @17:43:02.47 delay 5 seconds @17:43:07.49 Handoff.select SPR @17:43:07.61 SPR.command "start 2 14" @17:43:08.29 SPR stabilized @17:43:08.44 delay 10 seconds @17:43:18.46 SPR.command "start 1 0" @17:43:21.66 Waiting up to 4:00 for SPR to enter state 1 @17:45:25.92 SPR.command "hold_on" @17:45:27.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:45:27.92 Lysis1.seek 450uL @17:45:27.97 Lysis2.seek 450uL @17:46:10.05 delay 15 seconds @17:46:25.07 SPR.command "hold_off" @17:46:27.99 Waiting up to 38:00 for SPR to enter state 7 @18:04:27.60 SPRsummary:-60.8893,125.552,129.292,223.565 after 2005s @18:11:04.96 delay 10 seconds @18:11:14.98 SPR.command "start 1 0" @18:11:17.68 Waiting up to 4:00 for SPR to enter state 1 @18:13:21.96 SPR.command "hold_on" @18:13:23.36 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:23.93 Lysis1.seek 300uL @18:13:23.99 Lysis2.seek 300uL @18:13:45.11 delay 15 seconds @18:14:00.13 SPR.command "hold_off" @18:14:03.20 Waiting up to 38:00 for SPR to enter state 7 @18:32:58.79 Session Begun @18:33:02.64 -> showlog -50 @18:38:40.18 delay 10 seconds @18:38:50.20 SPR.command "start 1 0" @18:38:53.41 Waiting up to 4:00 for SPR to enter state 1 @18:40:58.13 SPR.command "hold_on" @18:40:59.51 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:41:00.10 Lysis1.seek 150uL @18:41:00.18 Lysis2.seek 150uL @18:41:21.31 delay 15 seconds @18:41:36.33 SPR.command "hold_off" @18:41:39.35 Waiting up to 38:00 for SPR to enter state 7 @18:56:59.59 Session Ended @19:06:16.33 delay 10 seconds @19:06:26.35 SPR.command "start 1 0" @19:06:29.56 Waiting up to 4:00 for SPR to enter state 1 @19:08:34.28 SPR.command "hold_on" @19:08:35.66 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:08:36.20 Lysis1.seek 0uL @19:08:36.26 Lysis2.seek 0uL @19:08:57.38 delay 15 seconds @19:09:12.40 SPR.command "hold_off" @19:09:15.51 Waiting up to 38:00 for SPR to enter state 7 @19:33:52.34 SPR analysis completed @19:33:54.40 Ended log spr23mar2617h.spr.bz2 @19:33:54.42 SPR.command "stats_off" @19:33:55.24 Intake.close @19:33:55.31 Exhaust.close @19:33:55.39 Bypass.close @19:33:55.49 Handoff.select :close @19:33:55.68
Gate.power :heat,:OFF @19:33:55.69 Gate.power :cartridge,:OFF @19:33:55.77 PinPort.in :unlocking @19:33:55.84 PinPort.seek :clear @19:33:56.30 Carousel.in :default @19:33:56.44 Carousel.home.seek 56 @19:33:56.79 Plunge.seek :locked @19:33:56.97 Archive.in :unlocking @19:33:57.12 Archive.seek :clear @19:33:57.20 Air.in :unlocking @19:33:57.38 Air.seek :clear @19:33:57.48 Lysis1.in :unlocking @19:33:57.63 Lysis1.seek :clear @19:33:57.69 Lysis2.in :unlocking @19:33:57.81 Lysis2.seek :clear @19:34:02.96
Gate.power :motor,:OFF @19:34:02.98 Gate.power :core,:OFF @19:34:02.99 Gate.power :analytic1,:OFF @19:34:03.00 Gate.power :analytic2,:OFF @19:34:03.02 Gate.power :analytic3,:OFF @19:34:03.03 Gate.power :main,:OFF @19:34:03.04 Safely stopped and ready to power off @19:34:06.80 Gate.power :main,:ON,limit to 5000mA @19:34:06.82 Gate.power :analytic1,:ON,limit to 5000mA @19:34:06.83 Gate.power :analytic2,:ON,limit to 5000mA @19:34:06.85 Gate.power :analytic3,:ON,limit to 5000mA @19:34:06.93 Powering core bus @19:34:09.28 SPR Module 1, FW Rev. B, FPGA Rev. C @19:34:09.69
Archive.configure :default @19:34:09.78 Air.configure :default @19:34:09.84 Lysis1.configure :default @19:34:09.91 Lysis2.configure :default @19:34:09.99 Plunge.configure :default @19:34:10.09 SamplePump.configure :default @19:34:10.18 Toroid.configure :default @19:34:10.27 Carousel.configure :default @19:34:10.35 Pressure.configure :default @19:34:10.44 ExtraPump.configure :default @19:34:10.51 PinPort.configure :default @19:34:10.57 All elf controllers running firmware version 4.83 @19:34:10.60 Gate.power :motor,:ON,limit to 5000mA @19:34:11.02 Archive.home.jog 0 @19:34:11.61 Carousel.home.jog 0 @19:34:11.66 Toroid.home.jog 0 @19:34:11.69 Lysis2.home.jog 0 @19:34:11.72 Lysis1.home.jog 0 @19:34:11.75 PinPort.home.jog 0 @19:34:11.77 Plunge.home.jog 0 @19:34:11.80 Air.home.jog 0 @19:34:13.05 Archive.in :homing @19:34:13.44 Archive.jog 1377 @19:34:13.48 Toroid.in :homing @19:34:13.51 Plunge.in :homing @19:34:13.55 Lysis1.in :homing @19:34:13.60 PinPort.in :homing @19:34:13.64 Lysis2.in :homing @19:34:13.88 Toroid.jog 619 @19:34:13.98 Lysis1.jog 1380 @19:34:14.02 Plunge.jog 1520 @19:34:14.07 Lysis2.jog 1387 @19:34:14.12 PinPort.jog -108 @19:34:14.40 Air.in :homing @19:34:14.46 Air.jog 1379 @19:34:14.54 PinPort.setRawPosition! 0 @19:34:14.64 PinPort.seek :home @19:34:14.73 PinPort.in :default @19:34:14.89 Archive.setRawPosition! 50 @19:34:14.94 Archive.seek :home @19:34:15.21 Archive.in :default @19:34:15.31 Lysis1.setRawPosition! 50 @19:34:15.38 Lysis2.setRawPosition! 50 @19:34:15.42 Lysis1.seek :home @19:34:15.51 Lysis2.seek :home @19:34:15.61 Plunge.setRawPosition! 50 @19:34:15.66 Plunge.seek :home @19:34:15.76 Air.setRawPosition! 50 @19:34:15.79 Lysis1.in :default @19:34:15.88 Air.seek :home @19:34:16.01 Lysis2.in :default @19:34:16.04 Plunge.in :default @19:34:16.20 Toroid.setRawPosition! 0 @19:34:16.23 Air.in :default @19:34:16.32 Toroid.seek :home @19:34:16.44 Toroid.in :default @19:34:16.64
Toroid.seek :clear @19:34:18.25 Carousel.in :default @19:34:18.37 Carousel.home.seek 56 @19:34:18.66 Selecting Cartridge 55 @19:34:18.95 Carousel.seek 55 @19:34:20.01 Lysis1.in :fast @19:34:20.07 Lysis1.seek :locked @19:34:22.72 Plunge.seek :locked @19:34:22.88 Air.in :fast @19:34:23.41 Air.seek :locked @19:34:24.99
Carousel.reconfigure :locked @19:34:25.15 Powering cartridge bus @19:34:27.79 Gate.setIR 1750,5000,0,6 @19:34:27.91 Gate.setIR 0,5000,0,6 @19:34:27.98 PuckHeater.configure :default @19:34:28.04 Gate.power :heat,:ON,limit to 5000mA @19:34:28.78 Mounted lysis Cartridge board#Q05048 @19:34:28.81 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:34:30.36 SPR.command "read_sequences" @19:34:30.89 SPR.command "stats_on" @19:34:31.05 SPR.command "start 0 1" @19:34:31.75 SPR.start /var/log/methane/spr23mar2619h.spr.bz2 @19:34:33.74 Waiting for baseline SPR stabilization @19:34:33.76 Handoff.select :waste @19:34:40.45 Syringe tare is 16% of full scale @19:34:40.48 PinPort.in :locking @19:34:40.54 PinPort.seek :locked @19:34:41.03 Archive.in :fast @19:34:41.14 Lysis2.in :fast @19:34:41.18 Lysis1.seek 1000uL @19:34:41.22 Archive.seek :full @19:34:41.38 Lysis2.seek 1000uL @19:34:45.93 delayUntil "19:44:33.31" @19:44:33.32 Lysis1.in :DAmix @19:44:33.45 Lysis2.in :DAmix @19:44:33.99 Lysis1.seek 750uL @19:44:34.04 Lysis2.seek 750uL @19:45:09.14 delay 5 seconds @19:45:14.16 Handoff.select SPR @19:45:14.29 SPR.command "start 2 14" @19:45:15.02 SPR stabilized @19:45:15.17 delay 10 seconds @19:45:25.19 SPR.command "start 1 0" @19:45:28.38 Waiting up to 4:00 for SPR to enter state 1 @19:47:32.68 SPR.command "hold_on" @19:47:34.06 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @19:47:34.67 Lysis1.seek 450uL @19:47:34.73 Lysis2.seek 450uL @19:48:16.80 delay 15 seconds @19:48:31.82 SPR.command "hold_off" @19:48:34.71 Waiting up to 38:00 for SPR to enter state 7 @20:06:34.32 SPRsummary:-8.30667,170.013,166.901,268.813 after 2005s @20:13:11.69 delay 10 seconds @20:13:21.71 SPR.command "start 1 0" @20:13:24.92 Waiting up to 4:00 for SPR to enter state 1 @20:15:29.64 SPR.command "hold_on" @20:15:31.55 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:15:32.13 Lysis1.seek 300uL @20:15:32.18 Lysis2.seek 300uL @20:15:53.28 delay 15 seconds @20:16:08.32 SPR.command "hold_off" @20:16:10.87 Waiting up to 38:00 for SPR to enter state 7 @20:40:46.90 delay 10 seconds @20:40:56.92 SPR.command "start 1 0" @20:41:00.13 Waiting up to 4:00 for SPR to enter state 1 @20:43:04.85 SPR.command "hold_on" @20:43:06.23 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:43:06.83 Lysis1.seek 150uL @20:43:06.88 Lysis2.seek 150uL @20:43:27.98 delay 15 seconds @20:43:43.00 SPR.command "hold_off" @20:43:46.08 Waiting up to 38:00 for SPR to enter state 7 @21:08:23.06 delay 10 seconds @21:08:33.08 SPR.command "start 1 0" @21:08:35.77 Waiting up to 4:00 for SPR to enter state 1 @21:10:40.06 SPR.command "hold_on" @21:10:41.44 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @21:10:42.01 Lysis1.seek 0uL @21:10:42.07 Lysis2.seek 0uL @21:11:03.17 delay 15 seconds @21:11:18.19 SPR.command "hold_off" @21:11:21.29 Waiting up to 38:00 for SPR to enter state 7 @21:35:58.13 SPR analysis completed @21:36:00.24 Ended log spr23mar2619h.spr.bz2 @21:36:00.26 SPR.command "stats_off" @21:36:00.65 Handoff.select :close @21:36:00.78
Gate.power :heat,:OFF @21:36:00.79 Gate.power :cartridge,:OFF @21:36:00.88 PinPort.in :unlocking @21:36:00.94 PinPort.seek :clear @21:36:01.41 Carousel.in :default @21:36:01.53 Carousel.home.seek 55 @21:36:01.87 Plunge.seek :locked @21:36:02.04 Archive.in :unlocking @21:36:02.19 Archive.seek :clear @21:36:02.32 Air.in :unlocking @21:36:02.45 Air.seek :clear @21:36:02.53 Lysis1.in :unlocking @21:36:02.74 Lysis1.seek :clear @21:36:02.79 Lysis2.in :unlocking @21:36:02.93 Lysis2.seek :clear @21:36:08.04
Gate.power :motor,:OFF @21:36:08.06 Gate.power :core,:OFF @21:36:08.07 Gate.power :analytic1,:OFF @21:36:08.08 Gate.power :analytic2,:OFF @21:36:08.10 Gate.power :analytic3,:OFF @21:36:08.11 Gate.power :main,:OFF @21:36:08.12 Safely stopped and ready to power off @21:36:11.88 Gate.power :main,:ON,limit to 5000mA @21:36:11.90 Gate.power :analytic1,:ON,limit to 5000mA @21:36:11.91 Gate.power :analytic2,:ON,limit to 5000mA @21:36:11.93 Gate.power :analytic3,:ON,limit to 5000mA @21:36:12.04 Powering core bus @21:36:14.35 SPR Module 1, FW Rev. B, FPGA Rev. C @21:36:14.82
Archive.configure :default @21:36:14.91 Air.configure :default @21:36:14.97 Lysis1.configure :default @21:36:15.04 Lysis2.configure :default @21:36:15.13 Plunge.configure :default @21:36:15.21 SamplePump.configure :default @21:36:15.31 Toroid.configure :default @21:36:15.40 Carousel.configure :default @21:36:15.48 Pressure.configure :default @21:36:15.58 ExtraPump.configure :default @21:36:15.64 PinPort.configure :default @21:36:15.70 All elf controllers running firmware version 4.83 @21:36:15.72 Gate.power :motor,:ON,limit to 5000mA @21:36:16.01 Archive.home.jog 0 @21:36:17.20 PinPort.home.jog 0 @21:36:17.25 Carousel.home.jog 0 @21:36:17.31 Toroid.home.jog 0 @21:36:17.35 Lysis2.home.jog 0 @21:36:17.37 Lysis1.home.jog 0 @21:36:17.40 Air.home.jog 0 @21:36:17.43 Plunge.home.jog 0 @21:36:18.26 Archive.in :homing @21:36:18.48 Archive.jog 1375 @21:36:18.55 PinPort.in :homing @21:36:18.58 Plunge.in :homing @21:36:18.63 Lysis1.in :homing @21:36:18.70 Toroid.in :homing @21:36:18.74 Air.in :homing @21:36:18.81 Lysis2.in :homing @21:36:19.03 Plunge.jog 1526 @21:36:19.08 PinPort.jog -109 @21:36:19.14 Lysis1.jog 1379 @21:36:19.18 Toroid.jog 627 @21:36:19.21 Air.jog 1381 @21:36:19.28 Lysis2.jog 1391 @21:36:19.75 PinPort.setRawPosition! 0 @21:36:19.80 PinPort.seek :home @21:36:19.89 PinPort.in :default @21:36:19.97 Archive.setRawPosition! 50 @21:36:20.03 Archive.seek :home @21:36:20.31 Archive.in :default @21:36:20.54 Plunge.setRawPosition! 50 @21:36:20.61 Plunge.seek :home @21:36:20.70 Lysis1.setRawPosition! 50 @21:36:20.78 Lysis1.seek :home @21:36:20.88 Lysis2.setRawPosition! 50 @21:36:20.91 Air.setRawPosition! 50 @21:36:20.98 Lysis2.seek :home @21:36:21.02 Air.seek :home @21:36:21.19 Lysis1.in :default @21:36:21.22 Plunge.in :default @21:36:21.39 Lysis2.in :default @21:36:21.42 Air.in :default @21:36:21.69 Toroid.setRawPosition! 0 @21:36:21.74 Toroid.seek :home @21:36:21.83 Toroid.in :default @21:36:21.91
Toroid.seek :clear @21:36:23.52 Carousel.in :default @21:36:23.65 Carousel.home.seek 55 @21:36:23.91 Selecting Cartridge 54 @21:36:24.20 Carousel.seek 54 @21:36:25.66 Lysis1.in :fast @21:36:25.81 Lysis1.seek :locked @21:36:28.44 Plunge.seek :locked @21:36:28.64 Air.in :fast @21:36:28.70 Air.seek :locked @21:36:30.28
Carousel.reconfigure :locked @21:36:30.43 Powering cartridge bus @21:36:33.07 Gate.setIR 1750,5000,0,6 @21:36:33.19 Gate.setIR 0,5000,0,6 @21:36:33.26 PuckHeater.configure :default @21:36:33.32 Gate.power :heat,:ON,limit to 5000mA @21:36:34.22 Mounted lysis Cartridge board#Q05045 @21:36:34.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:36:35.83 SPR.command "read_sequences" @21:36:35.97 SPR.command "stats_on" @21:36:36.13 SPR.command "start 0 1" @21:36:36.83 SPR.start /var/log/methane/spr23mar2621h.spr.bz2 @21:36:38.82 Waiting for baseline SPR stabilization @21:36:38.85 Handoff.select :waste @21:36:45.54 Syringe tare is 16% of full scale @21:36:45.56 PinPort.in :locking @21:36:45.62 PinPort.seek :locked @21:36:46.11 Archive.in :fast @21:36:46.22 Lysis2.in :fast @21:36:46.28 Lysis1.seek 1000uL @21:36:46.32 Archive.seek :full @21:36:46.43 Lysis2.seek 1000uL @21:36:51.00 delayUntil "21:46:38.39" @21:46:38.40 Lysis1.in :DAmix @21:46:38.53 Lysis2.in :DAmix @21:46:39.13 Lysis1.seek 750uL @21:46:39.21 Lysis2.seek 750uL @21:47:14.27 delay 5 seconds @21:47:19.29 Handoff.select SPR @21:47:19.42 SPR.command "start 2 14" @21:47:20.10 SPR stabilized @21:47:20.25 delay 10 seconds @21:47:30.27 SPR.command "start 1 0" @21:47:33.46 Waiting up to 4:00 for SPR to enter state 1 @21:49:37.76 SPR.command "hold_on" @21:49:39.14 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:49:39.73 Lysis1.seek 450uL @21:49:39.79 Lysis2.seek 450uL @21:50:21.85 delay 15 seconds @21:50:36.87 SPR.command "hold_off" @21:50:39.79 Waiting up to 38:00 for SPR to enter state 7 @22:08:39.40 SPRsummary:-4.59333,178.603,170.319,274.397 after 2005s @22:15:16.79 delay 10 seconds @22:15:26.81 SPR.command "start 1 0" @22:15:30.02 Waiting up to 4:00 for SPR to enter state 1 @22:16:24.97 Session Begun @22:16:30.47 -> showlog -100 @21:36:16.01 Archive.home.jog 0 @21:36:17.20 PinPort.home.jog 0 @21:36:17.25 Carousel.home.jog 0 @21:36:17.31 Toroid.home.jog 0 @21:36:17.35 Lysis2.home.jog 0 @21:36:17.37 Lysis1.home.jog 0 @21:36:17.40 Air.home.jog 0 @21:36:17.43 Plunge.home.jog 0 @21:36:18.26 Archive.in :homing @21:36:18.48 Archive.jog 1375 @21:36:18.55 PinPort.in :homing @21:36:18.58 Plunge.in :homing @21:36:18.63 Lysis1.in :homing @21:36:18.70 Toroid.in :homing @21:36:18.74 Air.in :homing @21:36:18.81 Lysis2.in :homing @21:36:19.03 Plunge.jog 1526 @21:36:19.08 PinPort.jog -109 @21:36:19.14 Lysis1.jog 1379 @21:36:19.18 Toroid.jog 627 @21:36:19.21 Air.jog 1381 @21:36:19.28 Lysis2.jog 1391 @21:36:19.75 PinPort.setRawPosition! 0 @21:36:19.80 PinPort.seek :home @21:36:19.89 PinPort.in :default @21:36:19.97 Archive.setRawPosition! 50 @21:36:20.03 Archive.seek :home @21:36:20.31 Archive.in :default @21:36:20.54 Plunge.setRawPosition! 50 @21:36:20.61 Plunge.seek :home @21:36:20.70 Lysis1.setRawPosition! 50 @21:36:20.78 Lysis1.seek :home @21:36:20.88 Lysis2.setRawPosition! 50 @21:36:20.91 Air.setRawPosition! 50 @21:36:20.98 Lysis2.seek :home @21:36:21.02 Air.seek :home @21:36:21.19 Lysis1.in :default @21:36:21.22 Plunge.in :default @21:36:21.39 Lysis2.in :default @21:36:21.42 Air.in :default @21:36:21.69 Toroid.setRawPosition! 0 @21:36:21.74 Toroid.seek :home @21:36:21.83 Toroid.in :default @21:36:21.91
Toroid.seek :clear @21:36:23.52 Carousel.in :default @21:36:23.65 Carousel.home.seek 55 @21:36:23.91 Selecting Cartridge 54 @21:36:24.20 Carousel.seek 54 @21:36:25.66 Lysis1.in :fast @21:36:25.81 Lysis1.seek :locked @21:36:28.44 Plunge.seek :locked @21:36:28.64 Air.in :fast @21:36:28.70 Air.seek :locked @21:36:30.28
Carousel.reconfigure :locked @21:36:30.43 Powering cartridge bus @21:36:33.07 Gate.setIR 1750,5000,0,6 @21:36:33.19 Gate.setIR 0,5000,0,6 @21:36:33.26 PuckHeater.configure :default @21:36:33.32 Gate.power :heat,:ON,limit to 5000mA @21:36:34.22 Mounted lysis Cartridge board#Q05045 @21:36:34.26 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:36:35.83 SPR.command "read_sequences" @21:36:35.97 SPR.command "stats_on" @21:36:36.13 SPR.command "start 0 1" @21:36:36.83 SPR.start /var/log/methane/spr23mar2621h.spr.bz2 @21:36:38.82 Waiting for baseline SPR stabilization @21:36:38.85 Handoff.select :waste @21:36:45.54 Syringe tare is 16% of full scale @21:36:45.56 PinPort.in :locking @21:36:45.62 PinPort.seek :locked @21:36:46.11 Archive.in :fast @21:36:46.22 Lysis2.in :fast @21:36:46.28 Lysis1.seek 1000uL @21:36:46.32 Archive.seek :full @21:36:46.43 Lysis2.seek 1000uL @21:36:51.00 delayUntil "21:46:38.39" @21:46:38.40 Lysis1.in :DAmix @21:46:38.53 Lysis2.in :DAmix @21:46:39.13 Lysis1.seek 750uL @21:46:39.21 Lysis2.seek 750uL @21:47:14.27 delay 5 seconds @21:47:19.29 Handoff.select SPR @21:47:19.42 SPR.command "start 2 14" @21:47:20.10 SPR stabilized @21:47:20.25 delay 10 seconds @21:47:30.27 SPR.command "start 1 0" @21:47:33.46 Waiting up to 4:00 for SPR to enter state 1 @21:49:37.76 SPR.command "hold_on" @21:49:39.14 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:49:39.73 Lysis1.seek 450uL @21:49:39.79 Lysis2.seek 450uL @21:50:21.85 delay 15 seconds @21:50:36.87 SPR.command "hold_off" @21:50:39.79 Waiting up to 38:00 for SPR to enter state 7 @22:08:39.40 SPRsummary:-4.59333,178.603,170.319,274.397 after 2005s @22:15:16.79 delay 10 seconds @22:15:26.81 SPR.command "start 1 0" @22:15:30.02 Waiting up to 4:00 for SPR to enter state 1 @22:16:24.97 Session Begun @22:16:30.47 -> showlog -100 @22:17:07.09 -> showlog -500 @22:17:34.72 SPR.command "hold_on" @22:17:36.10 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @22:17:36.78 Lysis1.seek 300uL @22:17:36.83 Lysis2.seek 300uL @22:17:57.99 delay 15 seconds @22:18:13.01 SPR.command "hold_off" @22:18:15.95 Waiting up to 38:00 for SPR to enter state 7 @22:18:42.23 -> exit @22:18:42.25 Session Ended @22:42:52.94 delay 10 seconds @22:43:02.96 SPR.command "start 1 0" @22:43:05.64 Waiting up to 4:00 for SPR to enter state 1 @22:45:09.93 SPR.command "hold_on" @22:45:11.31 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @22:45:11.88 Lysis1.seek 150uL @22:45:11.94 Lysis2.seek 150uL @22:45:33.05 delay 15 seconds @22:45:48.07 SPR.command "hold_off" @22:45:51.16 Waiting up to 38:00 for SPR to enter state 7 @23:10:28.14 delay 10 seconds @23:10:38.16 SPR.command "start 1 0" @23:10:40.84 Waiting up to 4:00 for SPR to enter state 1 @23:12:45.14 SPR.command "hold_on" @23:12:46.52 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @23:12:47.11 Lysis1.seek 0uL @23:12:47.17 Lysis2.seek 0uL @23:13:08.28 delay 15 seconds @23:13:23.30 SPR.command "hold_off" @23:13:26.37 Waiting up to 38:00 for SPR to enter state 7 @23:38:03.21 SPR analysis completed @23:38:05.31 Ended log spr23mar2621h.spr.bz2 @23:38:05.33 SPR.command "stats_off" @23:38:05.70 Handoff.select :close @23:38:05.84
Gate.power :heat,:OFF @23:38:05.85 Gate.power :cartridge,:OFF @23:38:05.94 PinPort.in :unlocking @23:38:06.00 PinPort.seek :clear @23:38:06.47 Carousel.in :default @23:38:06.62 Carousel.home.seek 54 @23:38:06.95 Plunge.seek :locked @23:38:07.12 Archive.in :unlocking @23:38:07.26 Archive.seek :clear @23:38:07.35 Air.in :unlocking @23:38:07.51 Air.seek :clear @23:38:07.64 Lysis1.in :unlocking @23:38:07.76 Lysis1.seek :clear @23:38:07.82 Lysis2.in :unlocking @23:38:07.94 Lysis2.seek :clear @23:38:13.10
Gate.power :motor,:OFF @23:38:13.12 Gate.power :core,:OFF @23:38:13.13 Gate.power :analytic1,:OFF @23:38:13.14 Gate.power :analytic2,:OFF @23:38:13.16 Gate.power :analytic3,:OFF @23:38:13.17 Gate.power :main,:OFF @23:38:13.18 Safely stopped and ready to power off @23:38:16.94 Gate.power :main,:ON,limit to 5000mA @23:38:16.96 Gate.power :analytic1,:ON,limit to 5000mA @23:38:16.97 Gate.power :analytic2,:ON,limit to 5000mA @23:38:16.99 Gate.power :analytic3,:ON,limit to 5000mA @23:38:17.33 Powering core bus @23:38:19.42 SPR Module 1, FW Rev. B, FPGA Rev. C @23:38:20.08
Archive.configure :default @23:38:20.17 Air.configure :default @23:38:20.24 Lysis1.configure :default @23:38:20.30 Lysis2.configure :default @23:38:20.39 Plunge.configure :default @23:38:20.48 SamplePump.configure :default @23:38:20.58 Toroid.configure :default @23:38:20.70 Carousel.configure :default @23:38:20.79 Pressure.configure :default @23:38:20.88 ExtraPump.configure :default @23:38:20.95 PinPort.configure :default @23:38:21.01 All elf controllers running firmware version 4.83 @23:38:21.03 Gate.power :motor,:ON,limit to 5000mA @23:38:21.24 Archive.home.jog 0 @23:38:22.01 Carousel.home.jog 0 @23:38:22.07 Toroid.home.jog 0 @23:38:22.62 Lysis2.home.jog 0 @23:38:22.68 Lysis1.home.jog 0 @23:38:22.72 Plunge.home.jog 0 @23:38:22.75 Air.home.jog 0 @23:38:22.79 PinPort.home.jog 0 @23:38:23.55 Archive.in :homing @23:38:23.86 Archive.jog 1374 @23:38:23.94 Toroid.in :homing @23:38:23.97 Plunge.in :homing @23:38:24.01 Lysis1.in :homing @23:38:24.05 PinPort.in :homing @23:38:24.09 Lysis2.in :homing @23:38:24.15 Air.in :homing @23:38:24.40 Plunge.jog 1520 @23:38:24.51 PinPort.jog -110 @23:38:24.55 Lysis2.jog 1385 @23:38:24.57 Air.jog 1382 @23:38:24.66 Toroid.jog 622 @23:38:24.72 Lysis1.jog 1382 @23:38:25.08 PinPort.setRawPosition! 0 @23:38:25.13 PinPort.seek :home @23:38:25.22 PinPort.in :default @23:38:25.34 Archive.setRawPosition! 50 @23:38:25.40 Archive.seek :home @23:38:25.67 Archive.in :default @23:38:25.93 Plunge.setRawPosition! 50 @23:38:25.98 Plunge.seek :home @23:38:26.11 Lysis2.setRawPosition! 50 @23:38:26.15 Air.setRawPosition! 50 @23:38:26.18 Lysis1.setRawPosition! 50 @23:38:26.28 Lysis2.seek :home @23:38:26.34 Air.seek :home @23:38:26.38 Lysis1.seek :home @23:38:26.58 Plunge.in :default @23:38:26.72 Lysis2.in :default @23:38:26.83 Lysis1.in :default @23:38:26.86 Air.in :default @23:38:27.02 Toroid.setRawPosition! 0 @23:38:27.08 Toroid.seek :home @23:38:27.16 Toroid.in :default @23:38:27.26
Toroid.seek :clear @23:38:28.87 Carousel.in :default @23:38:29.00 Carousel.home.seek 54 @23:38:29.26 Selecting Cartridge 53 @23:38:29.56 Carousel.seek 53 @23:38:31.12 Lysis1.in :fast @23:38:31.18 Lysis1.seek :locked @23:38:33.80 Plunge.seek :locked @23:38:33.96 Air.in :fast @23:38:34.03 Air.seek :locked @23:38:35.61
Carousel.reconfigure :locked @23:38:35.76 Powering cartridge bus @23:38:38.40 Gate.setIR 1750,5000,0,6 @23:38:38.52 Gate.setIR 0,5000,0,6 @23:38:38.60 PuckHeater.configure :default @23:38:38.68 Gate.power :heat,:ON,limit to 5000mA @23:38:39.41 Mounted lysis Cartridge board#Q05037 @23:38:39.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:38:40.98 SPR.command "read_sequences" @23:38:41.04 SPR.command "stats_on" @23:38:41.20 SPR.command "start 0 1" @23:38:41.90 SPR.start /var/log/methane/spr23mar2623h.spr.bz2 @23:38:43.89 Waiting for baseline SPR stabilization @23:38:43.91 Handoff.select :waste @23:38:50.63 Syringe tare is 16% of full scale @23:38:50.66 PinPort.in :locking @23:38:50.72 PinPort.seek :locked @23:38:51.20 Archive.in :fast @23:38:51.28 Lysis2.in :fast @23:38:51.35 Lysis1.seek 1000uL @23:38:51.45 Archive.seek :full @23:38:51.55 Lysis2.seek 1000uL @23:38:56.10 delayUntil "23:48:43.45" @23:48:43.46 Lysis1.in :DAmix @23:48:43.59 Lysis2.in :DAmix @23:48:44.16 Lysis1.seek 750uL @23:48:44.20 Lysis2.seek 750uL @23:49:19.35 delay 5 seconds @23:49:24.37 Handoff.select SPR @23:49:24.49 SPR.command "start 2 14" @23:49:25.16 SPR stabilized @23:49:25.31 delay 10 seconds @23:49:35.33 SPR.command "start 1 0" @23:49:39.04 Waiting up to 4:00 for SPR to enter state 1 @23:51:43.77 SPR.command "hold_on" @23:51:45.15 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @23:51:45.73 Lysis1.seek 450uL @23:51:45.79 Lysis2.seek 450uL @23:52:27.88 delay 15 seconds @23:52:42.90 SPR.command "hold_off" @23:52:45.80 Waiting up to 38:00 for SPR to enter state 7 @00:10:45.41PDT27-Mar-23 SPRsummary:-5.808,176.657,166.121,265.507 after 2006s @00:17:22.79 delay 10 seconds @00:17:32.81 SPR.command "start 1 0" @00:17:36.01 Waiting up to 4:00 for SPR to enter state 1 @00:19:40.73 SPR.command "hold_on" @00:19:42.11 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @00:19:42.75 Lysis1.seek 300uL @00:19:42.81 Lysis2.seek 300uL @00:20:03.90 delay 15 seconds @00:20:18.92 SPR.command "hold_off" @00:20:21.95 Waiting up to 38:00 for SPR to enter state 7 @00:44:58.93 delay 10 seconds @00:45:08.95 SPR.command "start 1 0" @00:45:12.16 Waiting up to 4:00 for SPR to enter state 1 @00:47:16.88 SPR.command "hold_on" @00:47:18.27 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @00:47:18.86 Lysis1.seek 150uL @00:47:18.92 Lysis2.seek 150uL @00:47:40.02 delay 15 seconds @00:47:55.04 SPR.command "hold_off" @00:47:58.11 Waiting up to 38:00 for SPR to enter state 7 @01:12:35.10 delay 10 seconds @01:12:45.12 SPR.command "start 1 0" @01:12:48.32 Waiting up to 4:00 for SPR to enter state 1 @01:14:53.04 SPR.command "hold_on" @01:14:54.42 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @01:14:55.03 Lysis1.seek 0uL @01:14:55.08 Lysis2.seek 0uL @01:15:16.18 delay 15 seconds @01:15:31.20 SPR.command "hold_off" @01:15:34.78 Waiting up to 38:00 for SPR to enter state 7 @01:40:12.05 SPR analysis completed @01:40:14.16 Ended log spr23mar2623h.spr.bz2 @01:40:14.18 SPR.command "stats_off" @01:40:14.55 Handoff.select :close @01:40:14.73
Gate.power :heat,:OFF @01:40:14.74 Gate.power :cartridge,:OFF @01:40:14.82 PinPort.in :unlocking @01:40:14.89 PinPort.seek :clear @01:40:15.36 Carousel.in :default @01:40:15.49 Carousel.home.seek 53 @01:40:15.81 Plunge.seek :locked @01:40:15.97 Archive.in :unlocking @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @11:24:40.49 -> showlog @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @11:24:40.49 -> showlog @11:25:26.93 SPR.command "hold_on" @11:25:28.31 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @11:25:29.01 Lysis1.seek 0uL @11:25:29.08 Lysis2.seek 0uL @11:25:50.24 delay 15 seconds @11:26:05.26 SPR.command "hold_off" @11:26:08.16 Waiting up to 38:00 for SPR to enter state 7 @11:50:44.99 SPR analysis completed @11:50:47.40 Ended log spr23mar2709h.spr.bz2 @11:50:47.42 SPR.command "stats_off" @11:50:48.85 Handoff.select :close @11:50:49.02
Gate.power :heat,:OFF @11:50:49.04 Gate.power :cartridge,:OFF @11:50:49.17 PinPort.in :unlocking @11:50:49.26 PinPort.seek :clear @11:50:49.75 Carousel.in :default @11:50:49.91 Carousel.home.seek 48 @11:50:50.76 Plunge.seek :locked @11:50:50.99 Archive.in :unlocking @11:50:51.18 Archive.seek :clear @11:50:51.32 Air.in :unlocking @11:50:51.47 Air.seek :clear @11:50:51.60 Lysis1.in :unlocking @11:50:51.78 Lysis1.seek :clear @11:50:51.90 Lysis2.in :unlocking @11:50:52.01 Lysis2.seek :clear @11:50:57.18
Gate.power :motor,:OFF @11:50:57.20 Gate.power :core,:OFF @11:50:57.25 Gate.power :analytic1,:OFF @11:50:57.29 Gate.power :analytic2,:OFF @11:50:57.32 Gate.power :analytic3,:OFF @11:50:57.36 Gate.power :main,:OFF @11:50:57.39 Safely stopped and ready to power off @11:51:01.06 Gate.power :main,:ON,limit to 5000mA @11:51:01.08 Gate.power :analytic1,:ON,limit to 5000mA @11:51:01.12 Gate.power :analytic2,:ON,limit to 5000mA @11:51:01.16 Gate.power :analytic3,:ON,limit to 5000mA @11:51:01.31 Powering core bus @11:51:03.63 SPR Module 1, FW Rev. B, FPGA Rev. C @11:51:04.08
Archive.configure :default @11:51:04.20 Air.configure :default @11:51:04.30 Lysis1.configure :default @11:51:04.39 Lysis2.configure :default @11:51:04.51 Plunge.configure :default @11:51:04.67 SamplePump.configure :default @11:51:04.79 Toroid.configure :default @11:51:04.91 Carousel.configure :default @11:51:05.03 Pressure.configure :default @11:51:05.15 ExtraPump.configure :default @11:51:05.23 PinPort.configure :default @11:51:05.33 All elf controllers running firmware version 4.83 @11:51:05.36 Gate.power :motor,:ON,limit to 5000mA @11:51:05.76 Archive.home.jog 0 @11:51:06.38 Carousel.home.jog 0 @11:51:06.44 Toroid.home.jog 0 @11:51:06.49 Plunge.home.jog 0 @11:51:06.54 Lysis1.home.jog 0 @11:51:06.64 PinPort.home.jog 0 @11:51:06.71 Lysis2.home.jog 0 @11:51:06.74 Air.home.jog 0 @11:51:08.12 Archive.in :homing @11:51:08.48 Archive.jog 1377 @11:51:08.56 Toroid.in :homing @11:51:08.65 Lysis2.in :homing @11:51:08.71 Air.in :homing @11:51:08.76 Plunge.in :homing @11:51:08.85 Lysis1.in :homing @11:51:08.90 PinPort.in :homing @11:51:09.29 Toroid.jog 619 @11:51:09.33 Plunge.jog 1521 @11:51:09.36 Lysis2.jog 1385 @11:51:09.40 Air.jog 1380 @11:51:09.47 PinPort.jog -109 @11:51:09.51 Lysis1.jog 1383 @11:51:09.99 PinPort.setRawPosition! 0 @11:51:10.07 PinPort.seek :home @11:51:10.16 Archive.setRawPosition! 50 @11:51:10.28 Archive.seek :home @11:51:10.39 PinPort.in :default @11:51:10.58 Archive.in :default @11:51:10.82 Lysis2.setRawPosition! 50 @11:51:10.94 Air.setRawPosition! 50 @11:51:10.98 Plunge.setRawPosition! 50 @11:51:11.02 Lysis2.seek :home @11:51:11.22 Plunge.seek :home @11:51:11.26 Air.seek :home @11:51:11.48 Lysis1.setRawPosition! 50 @11:51:11.51 Lysis2.in :default @11:51:11.63 Lysis1.seek :home @11:51:11.81 Toroid.setRawPosition! 0 @11:51:11.83 Plunge.in :default @11:51:11.90 Air.in :default @11:51:11.98 Toroid.seek :home @11:51:12.26 Lysis1.in :default @11:51:12.29 Toroid.in :default @11:51:12.70
Toroid.seek :clear @11:51:14.33 Carousel.in :default @11:51:14.48 Carousel.home.seek 48 @11:51:14.76 Selecting Cartridge 47 @11:51:15.10 Carousel.seek 47 @11:51:16.20 Lysis1.in :fast @11:51:16.30 Lysis1.seek :locked @11:51:18.95 Plunge.seek :locked @11:51:19.54 Air.in :fast @11:51:19.74 Air.seek :locked @11:51:21.35
Carousel.reconfigure :locked @11:51:21.54 Powering cartridge bus @11:51:24.21 Gate.setIR 1750,5000,0,6 @11:51:24.34 Gate.setIR 0,5000,0,6 @11:51:24.43 PuckHeater.configure :default @11:51:24.52 Gate.power :heat,:ON,limit to 5000mA @11:51:25.22 Mounted lysis Cartridge board#Q05053 @11:51:25.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:51:26.86 SPR.command "read_sequences" @11:51:27.07 SPR.command "stats_on" @11:51:27.26 SPR.command "start 0 1" @11:51:27.95 SPR.start /var/log/methane/spr23mar2711h.spr.bz2 @11:51:29.93 Waiting for baseline SPR stabilization @11:51:29.95 Handoff.select :waste @11:51:36.71 Syringe tare is 16% of full scale @11:51:36.73 PinPort.in :locking @11:51:36.86 PinPort.seek :locked @11:51:37.38 Archive.in :fast @11:51:37.49 Lysis2.in :fast @11:51:37.55 Archive.seek :full @11:51:37.65 Lysis1.seek 1000uL @11:51:37.83 Lysis2.seek 1000uL @11:51:42.44 delayUntil "12:01:29.49" @12:01:29.51 Lysis1.in :DAmix @12:01:29.67 Lysis2.in :DAmix @12:01:30.25 Lysis1.seek 750uL @12:01:30.31 Lysis2.seek 750uL @12:02:05.50 delay 5 seconds @12:02:10.52 Handoff.select SPR @12:02:10.67 SPR.command "start 2 14" @12:02:11.73 SPR stabilized @12:02:11.91 delay 10 seconds @12:02:21.94 SPR.command "start 1 0" @12:02:24.57 Waiting up to 4:00 for SPR to enter state 1 @12:04:28.86 SPR.command "hold_on" @12:04:30.25 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @12:04:30.94 Lysis1.seek 450uL @12:04:31.02 Lysis2.seek 450uL @12:05:13.13 delay 15 seconds @12:05:28.15 SPR.command "hold_off" @12:05:31.42 Waiting up to 38:00 for SPR to enter state 7 @12:06:53.91 Session Ended @12:23:31.45 SPRsummary:-2.55467,7.6,11.976,13.4213 after 2006s @12:30:08.84 delay 10 seconds @12:30:18.86 SPR.command "start 1 0" @12:30:22.05 Waiting up to 4:00 for SPR to enter state 1 @12:32:26.76 SPR.command "hold_on" @12:32:28.15 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @12:32:28.80 Lysis1.seek 300uL @12:32:28.87 Lysis2.seek 300uL @12:32:49.98 delay 15 seconds @12:33:05.01 SPR.command "hold_off" @12:33:08.00 Waiting up to 38:00 for SPR to enter state 7 @12:57:44.98 delay 10 seconds @12:57:55.00 SPR.command "start 1 0" @12:57:57.69 Waiting up to 4:00 for SPR to enter state 1 @13:00:01.98 SPR.command "hold_on" @13:00:03.88 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:00:04.47 Lysis2.seek 150uL @13:00:04.50 Lysis1.seek 150uL @13:00:25.61 delay 15 seconds @13:00:40.65 SPR.command "hold_off" @13:00:43.72 Waiting up to 38:00 for SPR to enter state 7 @13:25:20.20 delay 10 seconds @13:25:30.22 SPR.command "start 1 0" @13:25:32.90 Waiting up to 4:00 for SPR to enter state 1 @13:27:37.18 SPR.command "hold_on" @13:27:38.58 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:27:39.21 Lysis1.seek 0uL @13:27:39.27 Lysis2.seek 0uL @13:28:00.37 delay 15 seconds @13:28:15.39 SPR.command "hold_off" @13:28:18.42 Waiting up to 38:00 for SPR to enter state 7 @13:52:55.25 SPR analysis completed @13:52:57.42 Ended log spr23mar2711h.spr.bz2 @13:52:57.44 SPR.command "stats_off" @13:52:57.74 Handoff.select :close @13:52:58.33
Gate.power :heat,:OFF @13:52:58.35 Gate.power :cartridge,:OFF @13:52:58.43 PinPort.in :unlocking @13:52:58.50 PinPort.seek :clear @13:52:58.97 Carousel.in :default @13:52:59.10 Carousel.home.seek 47 @13:52:59.43 Plunge.seek :locked @13:52:59.59 Archive.in :unlocking @13:52:59.75 Archive.seek :clear @13:52:59.89 Air.in :unlocking @13:53:00.00 Air.seek :clear @13:53:00.07 Lysis1.in :unlocking @13:53:00.26 Lysis1.seek :clear @13:53:00.34 Lysis2.in :unlocking @13:53:00.44 Lysis2.seek :clear @13:53:05.57
Gate.power :motor,:OFF @13:53:05.59 Gate.power :core,:OFF @13:53:05.60 Gate.power :analytic1,:OFF @13:53:05.61 Gate.power :analytic2,:OFF @13:53:05.63 Gate.power :analytic3,:OFF @13:53:05.64 Gate.power :main,:OFF @13:53:05.65 Safely stopped and ready to power off @13:53:09.41 Gate.power :main,:ON,limit to 5000mA @13:53:09.43 Gate.power :analytic1,:ON,limit to 5000mA @13:53:09.45 Gate.power :analytic2,:ON,limit to 5000mA @13:53:09.46 Gate.power :analytic3,:ON,limit to 5000mA @13:53:09.70 Powering core bus @13:53:11.90 SPR Module 1, FW Rev. B, FPGA Rev. C @13:53:12.46
Archive.configure :default @13:53:12.56 Air.configure :default @13:53:12.65 Lysis1.configure :default @13:53:12.72 Lysis2.configure :default @13:53:12.82 Plunge.configure :default @13:53:12.91 SamplePump.configure :default @13:53:13.00 Toroid.configure :default @13:53:13.10 Carousel.configure :default @13:53:13.20 Pressure.configure :default @13:53:13.29 ExtraPump.configure :default @13:53:13.35 PinPort.configure :default @13:53:13.42 All elf controllers running firmware version 4.83 @13:53:13.44 Gate.power :motor,:ON,limit to 5000mA @13:53:13.75 Air.home.jog 0 @13:53:13.95 Archive.home.jog 0 @13:53:15.11 Carousel.home.jog 0 @13:53:15.15 Toroid.home.jog 0 @13:53:15.21 Lysis2.home.jog 0 @13:53:15.23 Lysis1.home.jog 0 @13:53:15.26 PinPort.home.jog 0 @13:53:15.29 Plunge.home.jog 0 @13:53:16.03 Air.in :homing @13:53:16.09 Archive.in :homing @13:53:16.31 Archive.jog 1376 @13:53:16.34 Air.jog 1377 @13:53:16.65 Plunge.in :homing @13:53:16.72 Lysis1.in :homing @13:53:16.75 Toroid.in :homing @13:53:16.80 Lysis2.in :homing @13:53:16.84 PinPort.in :homing @13:53:17.05 Lysis1.jog 1383 @13:53:17.16 PinPort.jog -111 @13:53:17.19 Toroid.jog 618 @13:53:17.21 Lysis2.jog 1389 @13:53:17.28 Plunge.jog 1519 @13:53:17.60 PinPort.setRawPosition! 0 @13:53:17.62 Air.setRawPosition! 50 @13:53:17.63 Archive.setRawPosition! 50 @13:53:17.76 PinPort.seek :home @13:53:17.80 Air.seek :home @13:53:17.83 Archive.seek :home @13:53:18.06 PinPort.in :default @13:53:18.15 Air.in :default @13:53:18.25 Archive.in :default @13:53:18.43 Lysis1.setRawPosition! 50 @13:53:18.48 Lysis1.seek :home @13:53:18.59 Lysis2.setRawPosition! 50 @13:53:18.67 Lysis2.seek :home @13:53:18.78 Plunge.setRawPosition! 50 @13:53:18.81 Lysis1.in :default @13:53:18.89 Plunge.seek :home @13:53:19.00 Lysis2.in :default @13:53:19.20 Plunge.in :default @13:53:19.47 Toroid.setRawPosition! 0 @13:53:19.52 Toroid.seek :home @13:53:19.61 Toroid.in :default @13:53:19.68
Toroid.seek :clear @13:53:21.29 Carousel.in :default @13:53:21.42 Carousel.home.seek 47 @13:53:21.68 Selecting Cartridge 46 @13:53:21.97 Carousel.seek 46 @13:53:23.55 Lysis1.in :fast @13:53:23.61 Lysis1.seek :locked @13:53:26.24 Plunge.seek :locked @13:53:26.41 Air.in :fast @13:53:26.48 Air.seek :locked @13:53:28.05
Carousel.reconfigure :locked @13:53:28.21 Powering cartridge bus @13:53:30.85 Gate.setIR 1750,5000,0,6 @13:53:30.98 Gate.setIR 0,5000,0,6 @13:53:31.04 PuckHeater.configure :default @13:53:31.10 Gate.power :heat,:ON,limit to 5000mA @13:53:31.92 Mounted lysis Cartridge board#Q05046 @13:53:31.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:53:33.49 SPR.command "read_sequences" @13:53:33.56 SPR.command "stats_on" @13:53:33.72 SPR.command "start 0 1" @13:53:34.42 SPR.start /var/log/methane/spr23mar2713h.spr.bz2 @13:53:36.35 Waiting for baseline SPR stabilization @13:53:36.38 Handoff.select :waste @13:53:43.08 Syringe tare is 16% of full scale @13:53:43.11 PinPort.in :locking @13:53:43.17 PinPort.seek :locked @13:53:43.66 Archive.in :fast @13:53:43.77 Lysis2.in :fast @13:53:43.82 Lysis1.seek 1000uL @13:53:43.86 Archive.seek :full @13:53:44.01 Lysis2.seek 1000uL @13:53:48.76 delayUntil "14:03:35.92" @14:03:35.94 Lysis1.in :DAmix @14:03:36.06 Lysis2.in :DAmix @14:03:36.68 Lysis1.seek 750uL @14:03:36.73 Lysis2.seek 750uL @14:04:11.90 delay 5 seconds @14:04:16.92 Handoff.select SPR @14:04:17.04 SPR.command "start 2 14" @14:04:17.63 SPR stabilized @14:04:17.78 delay 10 seconds @14:04:27.80 SPR.command "start 1 0" @14:04:31.52 Waiting up to 4:00 for SPR to enter state 1 @14:06:36.23 SPR.command "hold_on" @14:06:37.62 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:06:38.24 Lysis1.seek 450uL @14:06:38.29 Lysis2.seek 450uL @14:07:20.36 delay 15 seconds @14:07:35.38 SPR.command "hold_off" @14:07:38.27 Waiting up to 38:00 for SPR to enter state 7 @14:16:04.82 Session Begun @14:16:09.03 -> showlog @14:07:20.36 delay 15 seconds @14:07:35.38 SPR.command "hold_off" @14:07:38.27 Waiting up to 38:00 for SPR to enter state 7 @14:16:04.82 Session Begun @14:16:09.03 -> showlog @14:16:17.47 -> showlog -500 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @11:24:40.49 -> showlog @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @01:40:16.11 Archive.seek :clear @01:40:16.20 Air.in :unlocking @01:40:16.36 Air.seek :clear @01:40:16.48 Lysis1.in :unlocking @01:40:16.65 Lysis1.seek :clear @01:40:16.71 Lysis2.in :unlocking @01:40:16.82 Lysis2.seek :clear @01:40:21.95
Gate.power :motor,:OFF @01:40:21.96 Gate.power :core,:OFF @01:40:21.98 Gate.power :analytic1,:OFF @01:40:21.99 Gate.power :analytic2,:OFF @01:40:22.00 Gate.power :analytic3,:OFF @01:40:22.02 Gate.power :main,:OFF @01:40:22.03 Safely stopped and ready to power off @01:40:25.79 Gate.power :main,:ON,limit to 5000mA @01:40:25.80 Gate.power :analytic1,:ON,limit to 5000mA @01:40:25.82 Gate.power :analytic2,:ON,limit to 5000mA @01:40:25.83 Gate.power :analytic3,:ON,limit to 5000mA @01:40:26.17 Powering core bus @01:40:28.26 SPR Module 1, FW Rev. B, FPGA Rev. C @01:40:28.92
Archive.configure :default @01:40:29.02 Air.configure :default @01:40:29.08 Lysis1.configure :default @01:40:29.14 Lysis2.configure :default @01:40:29.23 Plunge.configure :default @01:40:29.32 SamplePump.configure :default @01:40:29.41 Toroid.configure :default @01:40:29.50 Carousel.configure :default @01:40:29.59 Pressure.configure :default @01:40:29.68 ExtraPump.configure :default @01:40:30.24 PinPort.configure :default @01:40:30.32 All elf controllers running firmware version 4.83 @01:40:30.34 Gate.power :motor,:ON,limit to 5000mA @01:40:30.72 Archive.home.jog 0 @01:40:31.34 PinPort.home.jog 0 @01:40:31.38 Carousel.home.jog 0 @01:40:31.43 Toroid.home.jog 0 @01:40:31.46 Plunge.home.jog 0 @01:40:31.49 Lysis2.home.jog 0 @01:40:31.52 Air.home.jog 0 @01:40:31.55 Lysis1.home.jog 0 @01:40:32.35 Archive.in :homing @01:40:32.58 Archive.jog 1374 @01:40:32.68 Plunge.in :homing @01:40:32.71 Lysis1.in :homing @01:40:32.77 PinPort.in :homing @01:40:32.80 Toroid.in :homing @01:40:32.84 Lysis2.in :homing @01:40:32.90 Air.in :homing @01:40:33.16 Plunge.jog 1523 @01:40:33.27 Lysis1.jog 1382 @01:40:33.31 PinPort.jog -110 @01:40:33.36 Toroid.jog 624 @01:40:33.40 Air.jog 1382 @01:40:33.48 Lysis2.jog 1384 @01:40:33.81 PinPort.setRawPosition! 0 @01:40:33.86 PinPort.seek :home @01:40:33.95 PinPort.in :default @01:40:34.15 Archive.setRawPosition! 50 @01:40:34.20 Archive.seek :home @01:40:34.48 Archive.in :default @01:40:34.68 Plunge.setRawPosition! 50 @01:40:34.70 Lysis1.setRawPosition! 50 @01:40:34.78 Plunge.seek :home @01:40:34.89 Lysis1.seek :home @01:40:35.00 Lysis2.setRawPosition! 50 @01:40:35.03 Air.setRawPosition! 50 @01:40:35.12 Lysis2.seek :home @01:40:35.15 Air.seek :home @01:40:35.33 Lysis1.in :default @01:40:35.36 Plunge.in :default @01:40:35.52 Lysis2.in :default @01:40:35.56 Air.in :default @01:40:35.72 Toroid.setRawPosition! 0 @01:40:35.77 Toroid.seek :home @01:40:35.86 Toroid.in :default @01:40:35.97
Toroid.seek :clear @01:40:37.58 Carousel.in :default @01:40:37.70 Carousel.home.seek 53 @01:40:38.41 Selecting Cartridge 52 @01:40:38.75 Carousel.seek 52 @01:40:39.82 Lysis1.in :fast @01:40:39.88 Lysis1.seek :locked @01:40:42.51 Plunge.seek :locked @01:40:42.70 Air.in :fast @01:40:42.76 Air.seek :locked @01:40:44.33
Carousel.reconfigure :locked @01:40:44.49 Powering cartridge bus @01:40:47.13 Gate.setIR 1750,5000,0,6 @01:40:47.25 Gate.setIR 0,5000,0,6 @01:40:47.32 PuckHeater.configure :default @01:40:47.38 Gate.power :heat,:ON,limit to 5000mA @01:40:48.10 Mounted lysis Cartridge board#Q05049 @01:40:48.13 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:40:49.69 SPR.command "read_sequences" @01:40:49.88 SPR.command "stats_on" @01:40:50.03 SPR.command "start 0 1" @01:40:50.74 SPR.start /var/log/methane/spr23mar2701h.spr.bz2 @01:40:52.73 Waiting for baseline SPR stabilization @01:40:52.75 Handoff.select :waste @01:40:59.44 Syringe tare is 16% of full scale @01:40:59.47 PinPort.in :locking @01:41:00.10 PinPort.seek :locked @01:41:00.60 Archive.in :fast @01:41:00.67 Lysis2.in :fast @01:41:00.75 Lysis1.seek 1000uL @01:41:00.97 Archive.seek :full @01:41:01.03 Lysis2.seek 1000uL @01:41:05.61 delayUntil "01:50:52.30" @01:50:52.31 Lysis1.in :DAmix @01:50:52.91 Lysis2.in :DAmix @01:50:53.40 Lysis1.seek 750uL @01:50:53.48 Lysis2.seek 750uL @01:51:28.54 delay 5 seconds @01:51:33.56 Handoff.select SPR @01:51:33.68 SPR.command "start 2 14" @01:51:35.47 SPR stabilized @01:51:35.63 delay 10 seconds @01:51:45.65 SPR.command "start 1 0" @01:51:48.84 Waiting up to 4:00 for SPR to enter state 1 @01:53:53.56 SPR.command "hold_on" @01:53:55.46 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:53:56.05 Lysis1.seek 450uL @01:53:56.09 Lysis2.seek 450uL @01:54:38.15 delay 15 seconds @01:54:53.17 SPR.command "hold_off" @01:54:56.54 Waiting up to 38:00 for SPR to enter state 7 @02:12:55.20 SPRsummary:-1.04133,122.433,113.905,183.565 after 2007s @02:19:32.57 delay 10 seconds @02:19:42.61 SPR.command "start 1 0" @02:19:45.80 Waiting up to 4:00 for SPR to enter state 1 @02:21:50.51 SPR.command "hold_on" @02:21:52.42 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:21:53.03 Lysis1.seek 300uL @02:21:53.08 Lysis2.seek 300uL @02:22:14.19 delay 15 seconds @02:22:29.21 SPR.command "hold_off" @02:22:31.75 Waiting up to 38:00 for SPR to enter state 7 @02:47:07.78 delay 10 seconds @02:47:17.81 SPR.command "start 1 0" @02:47:20.49 Waiting up to 4:00 for SPR to enter state 1 @02:49:24.78 SPR.command "hold_on" @02:49:26.69 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:49:27.23 Lysis1.seek 150uL @02:49:27.28 Lysis2.seek 150uL @02:49:48.41 delay 15 seconds @02:50:03.45 SPR.command "hold_off" @02:50:06.01 Waiting up to 38:00 for SPR to enter state 7 @03:14:42.04 delay 10 seconds @03:14:52.07 SPR.command "start 1 0" @03:14:54.75 Waiting up to 4:00 for SPR to enter state 1 @03:16:59.05 SPR.command "hold_on" @03:17:00.43 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:17:01.02 Lysis1.seek 0uL @03:17:01.08 Lysis2.seek 0uL @03:17:22.19 delay 15 seconds @03:17:37.21 SPR.command "hold_off" @03:17:40.79 Waiting up to 38:00 for SPR to enter state 7 @03:42:18.06 SPR analysis completed @03:42:20.19 Ended log spr23mar2701h.spr.bz2 @03:42:20.21 SPR.command "stats_off" @03:42:21.06 Handoff.select :close @03:42:21.21
Gate.power :heat,:OFF @03:42:21.23 Gate.power :cartridge,:OFF @03:42:21.31 PinPort.in :unlocking @03:42:21.37 PinPort.seek :clear @03:42:21.84 Carousel.in :default @03:42:21.97 Carousel.home.seek 52 @03:42:22.29 Plunge.seek :locked @03:42:22.46 Archive.in :unlocking @03:42:22.62 Archive.seek :clear @03:42:22.72 Air.in :unlocking @03:42:22.87 Air.seek :clear @03:42:22.98 Lysis1.in :unlocking @03:42:23.13 Lysis1.seek :clear @03:42:23.19 Lysis2.in :unlocking @03:42:23.31 Lysis2.seek :clear @03:42:28.42
Gate.power :motor,:OFF @03:42:28.44 Gate.power :core,:OFF @03:42:28.45 Gate.power :analytic1,:OFF @03:42:28.46 Gate.power :analytic2,:OFF @03:42:28.48 Gate.power :analytic3,:OFF @03:42:28.49 Gate.power :main,:OFF @03:42:28.50 Safely stopped and ready to power off @03:42:32.26 Gate.power :main,:ON,limit to 5000mA @03:42:32.28 Gate.power :analytic1,:ON,limit to 5000mA @03:42:32.29 Gate.power :analytic2,:ON,limit to 5000mA @03:42:32.31 Gate.power :analytic3,:ON,limit to 5000mA @03:42:32.42 Powering core bus @03:42:34.74 SPR Module 1, FW Rev. B, FPGA Rev. C @03:42:35.17
Archive.configure :default @03:42:35.26 Air.configure :default @03:42:35.32 Lysis1.configure :default @03:42:35.39 Lysis2.configure :default @03:42:35.48 Plunge.configure :default @03:42:35.57 SamplePump.configure :default @03:42:35.66 Toroid.configure :default @03:42:35.74 Carousel.configure :default @03:42:35.84 Pressure.configure :default @03:42:35.92 ExtraPump.configure :default @03:42:35.99 PinPort.configure :default @03:42:36.05 All elf controllers running firmware version 4.83 @03:42:36.07 Gate.power :motor,:ON,limit to 5000mA @03:42:36.92 Archive.home.jog 0 @03:42:37.59 PinPort.home.jog 0 @03:42:37.64 Carousel.home.jog 0 @03:42:37.66 Plunge.home.jog 0 @03:42:37.71 Lysis2.home.jog 0 @03:42:37.75 Air.home.jog 0 @03:42:37.77 Toroid.home.jog 0 @03:42:37.80 Lysis1.home.jog 0 @03:42:38.50 Archive.in :homing @03:42:38.92 Archive.jog 1376 @03:42:38.99 PinPort.in :homing @03:42:39.02 Toroid.in :homing @03:42:39.07 Lysis2.in :homing @03:42:39.11 Air.in :homing @03:42:39.15 Lysis1.in :homing @03:42:39.19 Plunge.in :homing @03:42:39.46 PinPort.jog -108 @03:42:39.50 Toroid.jog 626 @03:42:39.55 Lysis2.jog 1388 @03:42:39.73 Air.jog 1382 @03:42:39.83 Plunge.jog 1523 @03:42:39.88 Lysis1.jog 1382 @03:42:40.08 PinPort.setRawPosition! 0 @03:42:40.12 PinPort.seek :home @03:42:40.21 PinPort.in :default @03:42:40.46 Archive.setRawPosition! 50 @03:42:40.51 Archive.seek :home @03:42:40.79 Archive.in :default @03:42:40.91 Lysis2.setRawPosition! 50 @03:42:40.96 Lysis2.seek :home @03:42:41.07 Air.setRawPosition! 50 @03:42:41.12 Air.seek :home @03:42:41.18 Lysis1.setRawPosition! 50 @03:42:41.23 Plunge.setRawPosition! 50 @03:42:41.33 Lysis1.seek :home @03:42:41.40 Plunge.seek :home @03:42:41.60 Air.in :default @03:42:41.63 Lysis2.in :default @03:42:41.79 Plunge.in :default @03:42:41.84 Lysis1.in :default @03:42:41.96 Toroid.setRawPosition! 0 @03:42:42.02 Toroid.seek :home @03:42:42.11 Toroid.in :default @03:42:42.24
Toroid.seek :clear @03:42:43.85 Carousel.in :default @03:42:43.97 Carousel.home.seek 52 @03:42:44.23 Selecting Cartridge 51 @03:42:45.02 Carousel.seek 51 @03:42:46.09 Lysis1.in :fast @03:42:46.15 Lysis1.seek :locked @03:42:48.78 Plunge.seek :locked @03:42:48.94 Air.in :fast @03:42:49.01 Air.seek :locked @03:42:50.60
Carousel.reconfigure :locked @03:42:50.77 Powering cartridge bus @03:42:53.41 Gate.setIR 1750,5000,0,6 @03:42:53.53 Gate.setIR 0,5000,0,6 @03:42:53.60 PuckHeater.configure :default @03:42:53.66 Gate.power :heat,:ON,limit to 5000mA @03:42:54.37 Mounted lysis Cartridge board#Q05057 @03:42:54.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:42:56.03 SPR.command "read_sequences" @03:42:56.35 SPR.command "stats_on" @03:42:56.51 SPR.command "start 0 1" @03:42:57.21 SPR.start /var/log/methane/spr23mar2703h.spr.bz2 @03:42:59.72 Waiting for baseline SPR stabilization @03:42:59.74 Handoff.select :waste @03:43:06.43 Syringe tare is 16% of full scale @03:43:06.46 PinPort.in :locking @03:43:06.52 PinPort.seek :locked @03:43:07.18 Archive.in :fast @03:43:07.30 Lysis2.in :fast @03:43:07.35 Lysis1.seek 1000uL @03:43:07.45 Archive.seek :full @03:43:07.51 Lysis2.seek 1000uL @03:43:12.09 delayUntil "03:52:59.69" @03:52:59.71 Lysis1.in :DAmix @03:52:59.83 Lysis2.in :DAmix @03:53:00.41 Lysis1.seek 750uL @03:53:00.47 Lysis2.seek 750uL @03:53:35.56 delay 5 seconds @03:53:40.58 Handoff.select SPR @03:53:40.74 SPR.command "start 2 14" @03:53:41.42 SPR stabilized @03:53:41.58 delay 10 seconds @03:53:51.60 SPR.command "start 1 0" @03:53:54.79 Waiting up to 4:00 for SPR to enter state 1 @03:55:59.08 SPR.command "hold_on" @03:56:00.47 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:56:01.10 Lysis1.seek 450uL @03:56:01.16 Lysis2.seek 450uL @03:56:43.18 delay 15 seconds @03:56:58.21 SPR.command "hold_off" @03:57:01.12 Waiting up to 38:00 for SPR to enter state 7 @04:15:00.73 SPRsummary:-4.55733,72.4653,67.112,109.148 after 2006s @04:21:38.10 delay 10 seconds @04:21:48.13 SPR.command "start 1 0" @04:21:50.81 Waiting up to 4:00 for SPR to enter state 1 @04:23:55.10 SPR.command "hold_on" @04:23:56.49 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:23:57.14 Lysis1.seek 300uL @04:23:57.20 Lysis2.seek 300uL @04:24:18.30 delay 15 seconds @04:24:33.32 SPR.command "hold_off" @04:24:36.85 Waiting up to 38:00 for SPR to enter state 7 @04:49:14.26 delay 10 seconds @04:49:24.28 SPR.command "start 1 0" @04:49:26.96 Waiting up to 4:00 for SPR to enter state 1 @04:51:31.26 SPR.command "hold_on" @04:51:32.65 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:51:33.21 Lysis1.seek 150uL @04:51:33.30 Lysis2.seek 150uL @04:51:54.36 delay 15 seconds @04:52:09.38 SPR.command "hold_off" @04:52:13.00 Waiting up to 38:00 for SPR to enter state 7 @05:16:50.41 delay 10 seconds @05:17:00.43 SPR.command "start 1 0" @05:17:03.64 Waiting up to 4:00 for SPR to enter state 1 @05:19:08.36 SPR.command "hold_on" @05:19:09.74 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:19:10.32 Lysis1.seek 0uL @05:19:10.38 Lysis2.seek 0uL @05:19:31.47 delay 15 seconds @05:19:46.49 SPR.command "hold_off" @05:19:49.59 Waiting up to 38:00 for SPR to enter state 7 @05:44:26.41 SPR analysis completed @05:44:28.78 Ended log spr23mar2703h.spr.bz2 @05:44:28.79 SPR.command "stats_off" @05:44:29.43 Handoff.select :close @05:44:29.57
Gate.power :heat,:OFF @05:44:29.59 Gate.power :cartridge,:OFF @05:44:29.67 PinPort.in :unlocking @05:44:29.73 PinPort.seek :clear @05:44:30.20 Carousel.in :default @05:44:30.32 Carousel.home.seek 51 @05:44:30.68 Plunge.seek :locked @05:44:30.84 Archive.in :unlocking @05:44:30.97 Archive.seek :clear @05:44:31.09 Air.in :unlocking @05:44:31.23 Air.seek :clear @05:44:31.31 Lysis1.in :unlocking @05:44:31.49 Lysis1.seek :clear @05:44:31.55 Lysis2.in :unlocking @05:44:31.68 Lysis2.seek :clear @05:44:36.82
Gate.power :motor,:OFF @05:44:36.84 Gate.power :core,:OFF @05:44:36.85 Gate.power :analytic1,:OFF @05:44:36.86 Gate.power :analytic2,:OFF @05:44:36.88 Gate.power :analytic3,:OFF @05:44:36.89 Gate.power :main,:OFF @05:44:36.90 Safely stopped and ready to power off @05:44:40.66 Gate.power :main,:ON,limit to 5000mA @05:44:40.68 Gate.power :analytic1,:ON,limit to 5000mA @05:44:40.69 Gate.power :analytic2,:ON,limit to 5000mA @05:44:40.71 Gate.power :analytic3,:ON,limit to 5000mA @05:44:40.85 Powering core bus @05:44:43.13 SPR Module 1, FW Rev. B, FPGA Rev. C @05:44:43.60
Archive.configure :default @05:44:43.69 Air.configure :default @05:44:43.75 Lysis1.configure :default @05:44:43.82 Lysis2.configure :default @05:44:43.91 Plunge.configure :default @05:44:44.00 SamplePump.configure :default @05:44:44.09 Toroid.configure :default @05:44:44.18 Carousel.configure :default @05:44:44.28 Pressure.configure :default @05:44:44.37 ExtraPump.configure :default @05:44:44.94 PinPort.configure :default @05:44:45.05 All elf controllers running firmware version 4.83 @05:44:45.07 Gate.power :motor,:ON,limit to 5000mA @05:44:45.35 Archive.home.jog 0 @05:44:46.04 PinPort.home.jog 0 @05:44:46.08 Toroid.home.jog 0 @05:44:46.13 Plunge.home.jog 0 @05:44:46.17 Lysis1.home.jog 0 @05:44:46.19 Air.home.jog 0 @05:44:46.22 Carousel.home.jog 0 @05:44:46.26 Lysis2.home.jog 0 @05:44:46.98 Archive.in :homing @05:44:47.23 Archive.jog 1374 @05:44:47.44 Toroid.in :homing @05:44:47.51 Plunge.in :homing @05:44:47.55 Lysis1.in :homing @05:44:47.59 Lysis2.in :homing @05:44:47.63 Air.in :homing @05:44:47.67 PinPort.in :homing @05:44:47.92 Toroid.jog 624 @05:44:47.95 Plunge.jog 1525 @05:44:48.01 Lysis2.jog 1385 @05:44:48.05 Lysis1.jog 1383 @05:44:48.07 PinPort.jog -110 @05:44:48.10 Air.jog 1384 @05:44:48.51 Archive.setRawPosition! 50 @05:44:48.54 PinPort.setRawPosition! 0 @05:44:48.66 PinPort.seek :home @05:44:48.69 Archive.seek :home @05:44:48.85 PinPort.in :default @05:44:49.04 Archive.in :default @05:44:49.42 Plunge.setRawPosition! 50 @05:44:49.45 Lysis2.setRawPosition! 50 @05:44:49.47 Lysis1.setRawPosition! 50 @05:44:49.57 Plunge.seek :home @05:44:49.70 Lysis2.seek :home @05:44:49.76 Lysis1.seek :home @05:44:49.91 Air.setRawPosition! 50 @05:44:49.96 Air.seek :home @05:44:50.07 Plunge.in :default @05:44:50.17 Lysis2.in :default @05:44:50.20 Lysis1.in :default @05:44:50.36 Toroid.setRawPosition! 0 @05:44:50.39 Air.in :default @05:44:50.48 Toroid.seek :home @05:44:50.63 Toroid.in :default @05:44:50.81
Toroid.seek :clear @05:44:52.44 Carousel.in :default @05:44:52.56 Carousel.home.seek 51 @05:44:53.31 Selecting Cartridge 50 @05:44:53.64 Carousel.seek 50 @05:44:54.74 Lysis1.in :fast @05:44:54.80 Lysis1.seek :locked @05:44:57.43 Plunge.seek :locked @05:44:57.58 Air.in :fast @05:44:57.64 Air.seek :locked @05:44:59.21
Carousel.reconfigure :locked @05:44:59.38 Powering cartridge bus @05:45:02.02 Gate.setIR 1750,5000,0,6 @05:45:02.14 Gate.setIR 0,5000,0,6 @05:45:02.21 PuckHeater.configure :default @05:45:02.27 Gate.power :heat,:ON,limit to 5000mA @05:45:02.99 Mounted lysis Cartridge board#Q05060 @05:45:03.02 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:45:04.56 SPR.command "read_sequences" @05:45:04.75 SPR.command "stats_on" @05:45:04.90 SPR.command "start 0 1" @05:45:05.61 SPR.start /var/log/methane/spr23mar2705h.spr.bz2 @05:45:08.13 Waiting for baseline SPR stabilization @05:45:08.14 Handoff.select :waste @05:45:15.28 Syringe tare is 16% of full scale @05:45:15.41 PinPort.in :locking @05:45:15.47 PinPort.seek :locked @05:45:15.96 Archive.in :fast @05:45:16.05 Lysis2.in :fast @05:45:16.13 Lysis1.seek 1000uL @05:45:16.15 Archive.seek :full @05:45:16.35 Lysis2.seek 1000uL @05:45:20.84 delayUntil "05:55:08.09" @05:55:08.11 Lysis1.in :DAmix @05:55:08.23 Lysis2.in :DAmix @05:55:08.87 Lysis1.seek 750uL @05:55:08.91 Lysis2.seek 750uL @05:55:44.06 delay 5 seconds @05:55:49.08 Handoff.select SPR @05:55:49.21 SPR.command "start 2 14" @05:55:49.82 SPR stabilized @05:55:49.98 delay 10 seconds @05:56:00.00 SPR.command "start 1 0" @05:56:03.19 Waiting up to 4:00 for SPR to enter state 1 @05:58:07.48 SPR.command "hold_on" @05:58:08.87 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @05:58:09.47 Lysis1.seek 450uL @05:58:09.54 Lysis2.seek 450uL @05:58:51.60 delay 15 seconds @05:59:06.62 SPR.command "hold_off" @05:59:09.52 Waiting up to 38:00 for SPR to enter state 7 @06:17:09.13 SPRsummary:12.2693,21.6107,22.6533,35.112 after 2006s @06:23:46.51 delay 10 seconds @06:23:56.53 SPR.command "start 1 0" @06:23:59.73 Waiting up to 4:00 for SPR to enter state 1 @06:26:04.45 SPR.command "hold_on" @06:26:05.83 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:26:06.42 Lysis1.seek 300uL @06:26:06.51 Lysis2.seek 300uL @06:26:27.59 delay 15 seconds @06:26:42.61 SPR.command "hold_off" @06:26:45.67 Waiting up to 38:00 for SPR to enter state 7 @06:51:22.68 delay 10 seconds @06:51:32.70 SPR.command "start 1 0" @06:51:35.36 Waiting up to 4:00 for SPR to enter state 1 @06:53:39.65 SPR.command "hold_on" @06:53:41.04 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @06:53:41.62 Lysis1.seek 150uL @06:53:41.68 Lysis2.seek 150uL @06:54:02.79 delay 15 seconds @06:54:17.81 SPR.command "hold_off" @06:54:20.88 Waiting up to 38:00 for SPR to enter state 7 @07:18:57.86 delay 10 seconds @07:19:07.88 SPR.command "start 1 0" @07:19:11.09 Waiting up to 4:00 for SPR to enter state 1 @07:21:15.80 SPR.command "hold_on" @07:21:17.19 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:21:17.77 Lysis1.seek 0uL @07:21:17.84 Lysis2.seek 0uL @07:21:38.97 delay 15 seconds @07:21:53.99 SPR.command "hold_off" @07:21:57.04 Waiting up to 38:00 for SPR to enter state 7 @07:33:15.49 Session Begun @07:33:19.11 -> showlog -500 @07:34:48.42 -> exit @07:34:48.44 Session Ended @07:46:33.89 SPR analysis completed @07:46:35.95 Ended log spr23mar2705h.spr.bz2 @07:46:35.97 SPR.command "stats_off" @07:46:36.88 Handoff.select :close @07:46:37.02
Gate.power :heat,:OFF @07:46:37.03 Gate.power :cartridge,:OFF @07:46:37.12 PinPort.in :unlocking @07:46:37.18 PinPort.seek :clear @07:46:37.65 Carousel.in :default @07:46:37.78 Carousel.home.seek 50 @07:46:38.11 Plunge.seek :locked @07:46:38.28 Archive.in :unlocking @07:46:38.43 Archive.seek :clear @07:46:38.48 Air.in :unlocking @07:46:38.71 Air.seek :clear @07:46:38.77 Lysis1.in :unlocking @07:46:39.00 Lysis1.seek :clear @07:46:39.05 Lysis2.in :unlocking @07:46:39.16 Lysis2.seek :clear @07:46:44.30
Gate.power :motor,:OFF @07:46:44.31 Gate.power :core,:OFF @07:46:44.32 Gate.power :analytic1,:OFF @07:46:44.34 Gate.power :analytic2,:OFF @07:46:44.35 Gate.power :analytic3,:OFF @07:46:44.37 Gate.power :main,:OFF @07:46:44.38 Safely stopped and ready to power off @07:46:48.13 Gate.power :main,:ON,limit to 5000mA @07:46:48.15 Gate.power :analytic1,:ON,limit to 5000mA @07:46:48.16 Gate.power :analytic2,:ON,limit to 5000mA @07:46:48.18 Gate.power :analytic3,:ON,limit to 5000mA @07:46:48.32 Powering core bus @07:46:50.65 SPR Module 1, FW Rev. B, FPGA Rev. C @07:46:51.09
Archive.configure :default @07:46:51.18 Air.configure :default @07:46:51.24 Lysis1.configure :default @07:46:51.31 Lysis2.configure :default @07:46:51.40 Plunge.configure :default @07:46:51.49 SamplePump.configure :default @07:46:51.58 Toroid.configure :default @07:46:51.67 Carousel.configure :default @07:46:51.76 Pressure.configure :default @07:46:51.85 ExtraPump.configure :default @07:46:51.91 PinPort.configure :default @07:46:51.98 All elf controllers running firmware version 4.83 @07:46:52.00 Gate.power :motor,:ON,limit to 5000mA @07:46:52.72 Archive.home.jog 0 @07:46:53.54 PinPort.home.jog 0 @07:46:53.59 Toroid.home.jog 0 @07:46:53.62 Plunge.home.jog 0 @07:46:53.65 Lysis1.home.jog 0 @07:46:53.69 Air.home.jog 0 @07:46:53.71 Carousel.home.jog 0 @07:46:53.74 Lysis2.home.jog 0 @07:46:54.34 Archive.in :homing @07:46:54.83 PinPort.in :homing @07:46:54.88 Plunge.in :homing @07:46:54.92 Lysis1.in :homing @07:46:54.96 Toroid.in :homing @07:46:55.02 Archive.jog 1376 @07:46:55.24 PinPort.jog -111 @07:46:55.27 Plunge.jog 1525 @07:46:55.33 Lysis1.jog 1383 @07:46:55.38 Toroid.jog 626 @07:46:55.67 PinPort.setRawPosition! 0 @07:46:55.70 Air.in :homing @07:46:55.73 Lysis2.in :homing @07:46:55.86 PinPort.seek :home @07:46:55.92 Lysis2.jog 1388 @07:46:55.95 Air.jog 1380 @07:46:56.18 PinPort.in :default @07:46:56.33 Archive.setRawPosition! 50 @07:46:56.38 Archive.seek :home @07:46:56.61 Lysis1.setRawPosition! 50 @07:46:56.74 Plunge.setRawPosition! 50 @07:46:56.78 Lysis1.seek :home @07:46:56.80 Archive.in :default @07:46:56.94 Plunge.seek :home @07:46:57.17 Lysis2.setRawPosition! 50 @07:46:57.25 Lysis1.in :default @07:46:57.29 Lysis2.seek :home @07:46:57.44 Air.setRawPosition! 50 @07:46:57.47 Plunge.in :default @07:46:57.56 Air.seek :home @07:46:57.70 Lysis2.in :default @07:46:57.73 Toroid.setRawPosition! 0 @07:46:57.85 Toroid.seek :home @07:46:57.96 Toroid.in :default @07:46:57.99 Air.in :default @07:46:58.36
Toroid.seek :clear @07:46:59.98 Carousel.in :default @07:47:00.10 Carousel.home.seek 50 @07:47:00.37 Selecting Cartridge 49 @07:47:01.15 Carousel.seek 49 @07:47:02.22 Lysis1.in :fast @07:47:02.28 Lysis1.seek :locked @07:47:04.89 Plunge.seek :locked @07:47:05.05 Air.in :fast @07:47:05.12 Air.seek :locked @07:47:06.69
Carousel.reconfigure :locked @07:47:06.85 Powering cartridge bus @07:47:09.49 Gate.setIR 1750,5000,0,6 @07:47:09.61 Gate.setIR 0,5000,0,6 @07:47:09.68 PuckHeater.configure :default @07:47:09.74 Gate.power :heat,:ON,limit to 5000mA @07:47:10.47 Mounted lysis Cartridge board#Q05034 @07:47:10.51 Using this cartridge's heat loss curve for HeatingAccelInCarousel @07:47:12.09 SPR.command "read_sequences" @07:47:12.22 SPR.command "stats_on" @07:47:12.38 SPR.command "start 0 1" @07:47:13.08 SPR.start /var/log/methane/spr23mar2707h.spr.bz2 @07:47:15.08 Waiting for baseline SPR stabilization @07:47:15.10 Handoff.select :waste @07:47:21.79 Syringe tare is 16% of full scale @07:47:21.82 PinPort.in :locking @07:47:21.88 PinPort.seek :locked @07:47:22.37 Archive.in :fast @07:47:23.00 Lysis2.in :fast @07:47:23.04 Lysis1.seek 1000uL @07:47:23.23 Archive.seek :full @07:47:23.34 Lysis2.seek 1000uL @07:47:27.87 delayUntil "07:57:14.66" @07:57:14.68 Lysis1.in :DAmix @07:57:14.80 Lysis2.in :DAmix @07:57:15.39 Lysis1.seek 750uL @07:57:15.44 Lysis2.seek 750uL @07:57:50.61 delay 5 seconds @07:57:55.65 Handoff.select SPR @07:57:55.77 SPR.command "start 2 14" @07:57:56.35 SPR stabilized @07:57:56.51 delay 10 seconds @07:58:06.54 SPR.command "start 1 0" @07:58:10.24 Waiting up to 4:00 for SPR to enter state 1 @08:00:14.96 SPR.command "hold_on" @08:00:16.34 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @08:00:16.97 Lysis1.seek 450uL @08:00:17.03 Lysis2.seek 450uL @08:00:59.12 delay 15 seconds @08:01:14.14 SPR.command "hold_off" @08:01:16.99 Waiting up to 38:00 for SPR to enter state 7 @08:19:16.61 SPRsummary:-13.0507,18.3547,23.36,34.1867 after 2006s @08:25:53.97 delay 10 seconds @08:26:03.99 SPR.command "start 1 0" @08:26:06.68 Waiting up to 4:00 for SPR to enter state 1 @08:28:10.97 SPR.command "hold_on" @08:28:12.35 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:28:13.02 Lysis1.seek 300uL @08:28:13.06 Lysis2.seek 300uL @08:28:34.18 delay 15 seconds @08:28:49.20 SPR.command "hold_off" @08:28:52.20 Waiting up to 38:00 for SPR to enter state 7 @08:53:29.18 delay 10 seconds @08:53:39.20 SPR.command "start 1 0" @08:53:41.89 Waiting up to 4:00 for SPR to enter state 1 @08:55:46.17 SPR.command "hold_on" @08:55:47.56 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:55:48.15 Lysis1.seek 150uL @08:55:48.20 Lysis2.seek 150uL @08:56:09.34 delay 15 seconds @08:56:24.36 SPR.command "hold_off" @08:56:27.41 Waiting up to 38:00 for SPR to enter state 7 @09:21:04.38 delay 10 seconds @09:21:14.40 SPR.command "start 1 0" @09:21:17.10 Waiting up to 4:00 for SPR to enter state 1 @09:23:21.39 SPR.command "hold_on" @09:23:23.29 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @09:23:23.85 Lysis1.seek 0uL @09:23:23.89 Lysis2.seek 0uL @09:23:45.03 delay 15 seconds @09:24:00.07 SPR.command "hold_off" @09:24:02.65 Waiting up to 38:00 for SPR to enter state 7 @09:48:38.51 SPR analysis completed @09:48:40.74 Ended log spr23mar2707h.spr.bz2 @09:48:40.76 SPR.command "stats_off" @09:48:41.52 Handoff.select :close @09:48:41.66
Gate.power :heat,:OFF @09:48:41.67 Gate.power :cartridge,:OFF @09:48:41.75 PinPort.in :unlocking @09:48:41.81 PinPort.seek :clear @09:48:42.28 Carousel.in :default @09:48:42.41 Carousel.home.seek 49 @09:48:42.77 Plunge.seek :locked @09:48:42.93 Archive.in :unlocking @09:48:43.08 Archive.seek :clear @09:48:43.15 Air.in :unlocking @09:48:43.32 Air.seek :clear @09:48:43.39 Lysis1.in :unlocking @09:48:43.56 Lysis1.seek :clear @09:48:43.62 Lysis2.in :unlocking @09:48:43.75 Lysis2.seek :clear @09:48:48.89
Gate.power :motor,:OFF @09:48:48.90 Gate.power :core,:OFF @09:48:48.92 Gate.power :analytic1,:OFF @09:48:48.93 Gate.power :analytic2,:OFF @09:48:48.94 Gate.power :analytic3,:OFF @09:48:48.96 Gate.power :main,:OFF @09:48:48.97 Safely stopped and ready to power off @09:48:52.73 Gate.power :main,:ON,limit to 5000mA @09:48:52.74 Gate.power :analytic1,:ON,limit to 5000mA @09:48:52.76 Gate.power :analytic2,:ON,limit to 5000mA @09:48:52.77 Gate.power :analytic3,:ON,limit to 5000mA @09:48:52.92 Powering core bus @09:48:55.20 SPR Module 1, FW Rev. B, FPGA Rev. C @09:48:55.69
Archive.configure :default @09:48:55.78 Air.configure :default @09:48:55.85 Lysis1.configure :default @09:48:55.92 Lysis2.configure :default @09:48:56.02 Plunge.configure :default @09:48:56.12 SamplePump.configure :default @09:48:56.22 Toroid.configure :default @09:48:56.91 Carousel.configure :default @09:48:57.00 Pressure.configure :default @09:48:57.09 ExtraPump.configure :default @09:48:57.16 PinPort.configure :default @09:48:57.22 All elf controllers running firmware version 4.83 @09:48:57.24 Gate.power :motor,:ON,limit to 5000mA @09:48:57.52 Archive.home.jog 0 @09:48:58.21 Carousel.home.jog 0 @09:48:58.27 Toroid.home.jog 0 @09:48:58.29 Lysis2.home.jog 0 @09:48:58.32 Lysis1.home.jog 0 @09:48:58.35 Plunge.home.jog 0 @09:48:58.38 Air.home.jog 0 @09:48:58.42 PinPort.home.jog 0 @09:48:59.33 Archive.in :homing @09:48:59.60 Archive.jog 1376 @09:48:59.68 Toroid.in :homing @09:48:59.71 Lysis2.in :homing @09:48:59.75 Air.in :homing @09:48:59.79 Plunge.in :homing @09:48:59.85 Lysis1.in :homing @09:48:59.88 PinPort.in :homing @09:49:00.20 Toroid.jog 619 @09:49:00.23 Air.jog 1384 @09:49:00.28 Plunge.jog 1524 @09:49:00.31 Lysis2.jog 1390 @09:49:00.34 Lysis1.jog 1386 @09:49:00.37 PinPort.jog -108 @09:49:00.80 PinPort.setRawPosition! 0 @09:49:00.85 PinPort.seek :home @09:49:00.94 PinPort.in :default @09:49:01.09 Archive.setRawPosition! 50 @09:49:01.14 Archive.seek :home @09:49:01.42 Archive.in :default @09:49:01.68 Air.setRawPosition! 50 @09:49:01.77 Lysis2.setRawPosition! 50 @09:49:01.79 Air.seek :home @09:49:01.88 Lysis2.seek :home @09:49:01.99 Plunge.setRawPosition! 50 @09:49:02.02 Lysis1.setRawPosition! 50 @09:49:02.12 Plunge.seek :home @09:49:02.16 Lysis1.seek :home @09:49:02.30 Lysis2.in :default @09:49:02.33 Air.in :default @09:49:02.49 Lysis1.in :default @09:49:02.52 Plunge.in :default @09:49:02.70 Toroid.setRawPosition! 0 @09:49:02.75 Toroid.seek :home @09:49:02.86 Toroid.in :default @09:49:02.95
Toroid.seek :clear @09:49:04.98 Carousel.in :default @09:49:05.19 Carousel.home.seek 49 @09:49:05.45 Selecting Cartridge 48 @09:49:05.74 Carousel.seek 48 @09:49:06.82 Lysis1.in :fast @09:49:06.88 Lysis1.seek :locked @09:49:09.50 Plunge.seek :locked @09:49:09.67 Air.in :fast @09:49:09.74 Air.seek :locked @09:49:11.31
Carousel.reconfigure :locked @09:49:11.47 Powering cartridge bus @09:49:14.11 Gate.setIR 1750,5000,0,6 @09:49:14.23 Gate.setIR 0,5000,0,6 @09:49:14.30 PuckHeater.configure :default @09:49:14.36 Gate.power :heat,:ON,limit to 5000mA @09:49:15.06 Mounted lysis Cartridge board#Q05086 @09:49:15.09 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:49:16.64 SPR.command "read_sequences" @09:49:16.82 SPR.command "stats_on" @09:49:16.98 SPR.command "start 0 1" @09:49:17.68 SPR.start /var/log/methane/spr23mar2709h.spr.bz2 @09:49:19.67 Waiting for baseline SPR stabilization @09:49:19.69 Handoff.select :waste @09:49:26.38 Syringe tare is 16% of full scale @09:49:26.41 PinPort.in :locking @09:49:26.47 PinPort.seek :locked @09:49:26.96 Archive.in :fast @09:49:27.02 Lysis2.in :fast @09:49:27.09 Lysis1.seek 1000uL @09:49:27.24 Lysis2.seek 1000uL @09:49:27.30 Archive.seek :full @09:49:31.84 delayUntil "09:59:19.23" @09:59:19.25 Lysis1.in :DAmix @09:59:19.37 Lysis2.in :DAmix @09:59:19.96 Lysis1.seek 750uL @09:59:20.01 Lysis2.seek 750uL @09:59:55.15 delay 5 seconds @10:00:00.17 Handoff.select SPR @10:00:00.30 SPR.command "start 2 14" @10:00:01.46 SPR stabilized @10:00:01.63 delay 10 seconds @10:00:11.65 SPR.command "start 1 0" @10:00:14.31 Waiting up to 4:00 for SPR to enter state 1 @10:02:18.61 SPR.command "hold_on" @10:02:19.99 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @10:02:20.59 Lysis1.seek 450uL @10:02:20.70 Lysis2.seek 450uL @10:03:02.78 delay 15 seconds @10:03:17.80 SPR.command "hold_off" @10:03:21.16 Waiting up to 38:00 for SPR to enter state 7 @10:21:21.20 SPRsummary:-0.510667,11.7253,15.4013,19.784 after 2006s @10:27:58.56 delay 10 seconds @10:28:08.58 SPR.command "start 1 0" @10:28:11.27 Waiting up to 4:00 for SPR to enter state 1 @10:30:15.56 SPR.command "hold_on" @10:30:16.95 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:30:17.56 Lysis1.seek 300uL @10:30:17.63 Lysis2.seek 300uL @10:30:38.74 delay 15 seconds @10:30:53.76 SPR.command "hold_off" @10:30:56.80 Waiting up to 38:00 for SPR to enter state 7 @10:55:33.77 delay 10 seconds @10:55:43.79 SPR.command "start 1 0" @10:55:47.00 Waiting up to 4:00 for SPR to enter state 1 @10:57:51.72 SPR.command "hold_on" @10:57:53.11 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:57:53.69 Lysis1.seek 150uL @10:57:53.73 Lysis2.seek 150uL @10:58:14.85 delay 15 seconds @10:58:29.87 SPR.command "hold_off" @10:58:32.95 Waiting up to 38:00 for SPR to enter state 7 @11:22:22.26 Session Begun @11:22:26.46 -> showlog -500 @11:22:59.91 -> showlog @11:23:09.96 delay 10 seconds @11:23:19.99 SPR.command "start 1 0" @11:23:22.67 Waiting up to 4:00 for SPR to enter state 1 @11:24:25.01 -> showlog -50 @11:24:40.49 -> showlog @11:25:26.93 SPR.command "hold_on" @11:25:28.31 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @11:25:29.01 Lysis1.seek 0uL @11:25:29.08 Lysis2.seek 0uL @11:25:50.24 delay 15 seconds @11:26:05.26 SPR.command "hold_off" @11:26:08.16 Waiting up to 38:00 for SPR to enter state 7 @11:50:44.99 SPR analysis completed @11:50:47.40 Ended log spr23mar2709h.spr.bz2 @11:50:47.42 SPR.command "stats_off" @11:50:48.85 Handoff.select :close @11:50:49.02
Gate.power :heat,:OFF @11:50:49.04 Gate.power :cartridge,:OFF @11:50:49.17 PinPort.in :unlocking @11:50:49.26 PinPort.seek :clear @11:50:49.75 Carousel.in :default @11:50:49.91 Carousel.home.seek 48 @11:50:50.76 Plunge.seek :locked @11:50:50.99 Archive.in :unlocking @11:50:51.18 Archive.seek :clear @11:50:51.32 Air.in :unlocking @11:50:51.47 Air.seek :clear @11:50:51.60 Lysis1.in :unlocking @11:50:51.78 Lysis1.seek :clear @11:50:51.90 Lysis2.in :unlocking @11:50:52.01 Lysis2.seek :clear @11:50:57.18
Gate.power :motor,:OFF @11:50:57.20 Gate.power :core,:OFF @11:50:57.25 Gate.power :analytic1,:OFF @11:50:57.29 Gate.power :analytic2,:OFF @11:50:57.32 Gate.power :analytic3,:OFF @11:50:57.36 Gate.power :main,:OFF @11:50:57.39 Safely stopped and ready to power off @11:51:01.06 Gate.power :main,:ON,limit to 5000mA @11:51:01.08 Gate.power :analytic1,:ON,limit to 5000mA @11:51:01.12 Gate.power :analytic2,:ON,limit to 5000mA @11:51:01.16 Gate.power :analytic3,:ON,limit to 5000mA @11:51:01.31 Powering core bus @11:51:03.63 SPR Module 1, FW Rev. B, FPGA Rev. C @11:51:04.08
Archive.configure :default @11:51:04.20 Air.configure :default @11:51:04.30 Lysis1.configure :default @11:51:04.39 Lysis2.configure :default @11:51:04.51 Plunge.configure :default @11:51:04.67 SamplePump.configure :default @11:51:04.79 Toroid.configure :default @11:51:04.91 Carousel.configure :default @11:51:05.03 Pressure.configure :default @11:51:05.15 ExtraPump.configure :default @11:51:05.23 PinPort.configure :default @11:51:05.33 All elf controllers running firmware version 4.83 @11:51:05.36 Gate.power :motor,:ON,limit to 5000mA @11:51:05.76 Archive.home.jog 0 @11:51:06.38 Carousel.home.jog 0 @11:51:06.44 Toroid.home.jog 0 @11:51:06.49 Plunge.home.jog 0 @11:51:06.54 Lysis1.home.jog 0 @11:51:06.64 PinPort.home.jog 0 @11:51:06.71 Lysis2.home.jog 0 @11:51:06.74 Air.home.jog 0 @11:51:08.12 Archive.in :homing @11:51:08.48 Archive.jog 1377 @11:51:08.56 Toroid.in :homing @11:51:08.65 Lysis2.in :homing @11:51:08.71 Air.in :homing @11:51:08.76 Plunge.in :homing @11:51:08.85 Lysis1.in :homing @11:51:08.90 PinPort.in :homing @11:51:09.29 Toroid.jog 619 @11:51:09.33 Plunge.jog 1521 @11:51:09.36 Lysis2.jog 1385 @11:51:09.40 Air.jog 1380 @11:51:09.47 PinPort.jog -109 @11:51:09.51 Lysis1.jog 1383 @11:51:09.99 PinPort.setRawPosition! 0 @11:51:10.07 PinPort.seek :home @11:51:10.16 Archive.setRawPosition! 50 @11:51:10.28 Archive.seek :home @11:51:10.39 PinPort.in :default @11:51:10.58 Archive.in :default @11:51:10.82 Lysis2.setRawPosition! 50 @11:51:10.94 Air.setRawPosition! 50 @11:51:10.98 Plunge.setRawPosition! 50 @11:51:11.02 Lysis2.seek :home @11:51:11.22 Plunge.seek :home @11:51:11.26 Air.seek :home @11:51:11.48 Lysis1.setRawPosition! 50 @11:51:11.51 Lysis2.in :default @11:51:11.63 Lysis1.seek :home @11:51:11.81 Toroid.setRawPosition! 0 @11:51:11.83 Plunge.in :default @11:51:11.90 Air.in :default @11:51:11.98 Toroid.seek :home @11:51:12.26 Lysis1.in :default @11:51:12.29 Toroid.in :default @11:51:12.70
Toroid.seek :clear @11:51:14.33 Carousel.in :default @11:51:14.48 Carousel.home.seek 48 @11:51:14.76 Selecting Cartridge 47 @11:51:15.10 Carousel.seek 47 @11:51:16.20 Lysis1.in :fast @11:51:16.30 Lysis1.seek :locked @11:51:18.95 Plunge.seek :locked @11:51:19.54 Air.in :fast @11:51:19.74 Air.seek :locked @11:51:21.35
Carousel.reconfigure :locked @11:51:21.54 Powering cartridge bus @11:51:24.21 Gate.setIR 1750,5000,0,6 @11:51:24.34 Gate.setIR 0,5000,0,6 @11:51:24.43 PuckHeater.configure :default @11:51:24.52 Gate.power :heat,:ON,limit to 5000mA @11:51:25.22 Mounted lysis Cartridge board#Q05053 @11:51:25.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:51:26.86 SPR.command "read_sequences" @11:51:27.07 SPR.command "stats_on" @11:51:27.26 SPR.command "start 0 1" @11:51:27.95 SPR.start /var/log/methane/spr23mar2711h.spr.bz2 @11:51:29.93 Waiting for baseline SPR stabilization @11:51:29.95 Handoff.select :waste @11:51:36.71 Syringe tare is 16% of full scale @11:51:36.73 PinPort.in :locking @11:51:36.86 PinPort.seek :locked @11:51:37.38 Archive.in :fast @11:51:37.49 Lysis2.in :fast @11:51:37.55 Archive.seek :full @11:51:37.65 Lysis1.seek 1000uL @11:51:37.83 Lysis2.seek 1000uL @11:51:42.44 delayUntil "12:01:29.49" @12:01:29.51 Lysis1.in :DAmix @12:01:29.67 Lysis2.in :DAmix @12:01:30.25 Lysis1.seek 750uL @12:01:30.31 Lysis2.seek 750uL @12:02:05.50 delay 5 seconds @12:02:10.52 Handoff.select SPR @12:02:10.67 SPR.command "start 2 14" @12:02:11.73 SPR stabilized @12:02:11.91 delay 10 seconds @12:02:21.94 SPR.command "start 1 0" @12:02:24.57 Waiting up to 4:00 for SPR to enter state 1 @12:04:28.86 SPR.command "hold_on" @12:04:30.25 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @12:04:30.94 Lysis1.seek 450uL @12:04:31.02 Lysis2.seek 450uL @12:05:13.13 delay 15 seconds @12:05:28.15 SPR.command "hold_off" @12:05:31.42 Waiting up to 38:00 for SPR to enter state 7 @12:06:53.91 Session Ended @12:23:31.45 SPRsummary:-2.55467,7.6,11.976,13.4213 after 2006s @12:30:08.84 delay 10 seconds @12:30:18.86 SPR.command "start 1 0" @12:30:22.05 Waiting up to 4:00 for SPR to enter state 1 @12:32:26.76 SPR.command "hold_on" @12:32:28.15 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @12:32:28.80 Lysis1.seek 300uL @12:32:28.87 Lysis2.seek 300uL @12:32:49.98 delay 15 seconds @12:33:05.01 SPR.command "hold_off" @12:33:08.00 Waiting up to 38:00 for SPR to enter state 7 @12:57:44.98 delay 10 seconds @12:57:55.00 SPR.command "start 1 0" @12:57:57.69 Waiting up to 4:00 for SPR to enter state 1 @13:00:01.98 SPR.command "hold_on" @13:00:03.88 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:00:04.47 Lysis2.seek 150uL @13:00:04.50 Lysis1.seek 150uL @13:00:25.61 delay 15 seconds @13:00:40.65 SPR.command "hold_off" @13:00:43.72 Waiting up to 38:00 for SPR to enter state 7 @13:25:20.20 delay 10 seconds @13:25:30.22 SPR.command "start 1 0" @13:25:32.90 Waiting up to 4:00 for SPR to enter state 1 @13:27:37.18 SPR.command "hold_on" @13:27:38.58 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:27:39.21 Lysis1.seek 0uL @13:27:39.27 Lysis2.seek 0uL @13:28:00.37 delay 15 seconds @13:28:15.39 SPR.command "hold_off" @13:28:18.42 Waiting up to 38:00 for SPR to enter state 7 @13:52:55.25 SPR analysis completed @13:52:57.42 Ended log spr23mar2711h.spr.bz2 @13:52:57.44 SPR.command "stats_off" @13:52:57.74 Handoff.select :close @13:52:58.33
Gate.power :heat,:OFF @13:52:58.35 Gate.power :cartridge,:OFF @13:52:58.43 PinPort.in :unlocking @13:52:58.50 PinPort.seek :clear @13:52:58.97 Carousel.in :default @13:52:59.10 Carousel.home.seek 47 @13:52:59.43 Plunge.seek :locked @13:52:59.59 Archive.in :unlocking @13:52:59.75 Archive.seek :clear @13:52:59.89 Air.in :unlocking @13:53:00.00 Air.seek :clear @13:53:00.07 Lysis1.in :unlocking @13:53:00.26 Lysis1.seek :clear @13:53:00.34 Lysis2.in :unlocking @13:53:00.44 Lysis2.seek :clear @13:53:05.57
Gate.power :motor,:OFF @13:53:05.59 Gate.power :core,:OFF @13:53:05.60 Gate.power :analytic1,:OFF @13:53:05.61 Gate.power :analytic2,:OFF @13:53:05.63 Gate.power :analytic3,:OFF @13:53:05.64 Gate.power :main,:OFF @13:53:05.65 Safely stopped and ready to power off @13:53:09.41 Gate.power :main,:ON,limit to 5000mA @13:53:09.43 Gate.power :analytic1,:ON,limit to 5000mA @13:53:09.45 Gate.power :analytic2,:ON,limit to 5000mA @13:53:09.46 Gate.power :analytic3,:ON,limit to 5000mA @13:53:09.70 Powering core bus @13:53:11.90 SPR Module 1, FW Rev. B, FPGA Rev. C @13:53:12.46
Archive.configure :default @13:53:12.56 Air.configure :default @13:53:12.65 Lysis1.configure :default @13:53:12.72 Lysis2.configure :default @13:53:12.82 Plunge.configure :default @13:53:12.91 SamplePump.configure :default @13:53:13.00 Toroid.configure :default @13:53:13.10 Carousel.configure :default @13:53:13.20 Pressure.configure :default @13:53:13.29 ExtraPump.configure :default @13:53:13.35 PinPort.configure :default @13:53:13.42 All elf controllers running firmware version 4.83 @13:53:13.44 Gate.power :motor,:ON,limit to 5000mA @13:53:13.75 Air.home.jog 0 @13:53:13.95 Archive.home.jog 0 @13:53:15.11 Carousel.home.jog 0 @13:53:15.15 Toroid.home.jog 0 @13:53:15.21 Lysis2.home.jog 0 @13:53:15.23 Lysis1.home.jog 0 @13:53:15.26 PinPort.home.jog 0 @13:53:15.29 Plunge.home.jog 0 @13:53:16.03 Air.in :homing @13:53:16.09 Archive.in :homing @13:53:16.31 Archive.jog 1376 @13:53:16.34 Air.jog 1377 @13:53:16.65 Plunge.in :homing @13:53:16.72 Lysis1.in :homing @13:53:16.75 Toroid.in :homing @13:53:16.80 Lysis2.in :homing @13:53:16.84 PinPort.in :homing @13:53:17.05 Lysis1.jog 1383 @13:53:17.16 PinPort.jog -111 @13:53:17.19 Toroid.jog 618 @13:53:17.21 Lysis2.jog 1389 @13:53:17.28 Plunge.jog 1519 @13:53:17.60 PinPort.setRawPosition! 0 @13:53:17.62 Air.setRawPosition! 50 @13:53:17.63 Archive.setRawPosition! 50 @13:53:17.76 PinPort.seek :home @13:53:17.80 Air.seek :home @13:53:17.83 Archive.seek :home @13:53:18.06 PinPort.in :default @13:53:18.15 Air.in :default @13:53:18.25 Archive.in :default @13:53:18.43 Lysis1.setRawPosition! 50 @13:53:18.48 Lysis1.seek :home @13:53:18.59 Lysis2.setRawPosition! 50 @13:53:18.67 Lysis2.seek :home @13:53:18.78 Plunge.setRawPosition! 50 @13:53:18.81 Lysis1.in :default @13:53:18.89 Plunge.seek :home @13:53:19.00 Lysis2.in :default @13:53:19.20 Plunge.in :default @13:53:19.47 Toroid.setRawPosition! 0 @13:53:19.52 Toroid.seek :home @13:53:19.61 Toroid.in :default @13:53:19.68
Toroid.seek :clear @13:53:21.29 Carousel.in :default @13:53:21.42 Carousel.home.seek 47 @13:53:21.68 Selecting Cartridge 46 @13:53:21.97 Carousel.seek 46 @13:53:23.55 Lysis1.in :fast @13:53:23.61 Lysis1.seek :locked @13:53:26.24 Plunge.seek :locked @13:53:26.41 Air.in :fast @13:53:26.48 Air.seek :locked @13:53:28.05
Carousel.reconfigure :locked @13:53:28.21 Powering cartridge bus @13:53:30.85 Gate.setIR 1750,5000,0,6 @13:53:30.98 Gate.setIR 0,5000,0,6 @13:53:31.04 PuckHeater.configure :default @13:53:31.10 Gate.power :heat,:ON,limit to 5000mA @13:53:31.92 Mounted lysis Cartridge board#Q05046 @13:53:31.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:53:33.49 SPR.command "read_sequences" @13:53:33.56 SPR.command "stats_on" @13:53:33.72 SPR.command "start 0 1" @13:53:34.42 SPR.start /var/log/methane/spr23mar2713h.spr.bz2 @13:53:36.35 Waiting for baseline SPR stabilization @13:53:36.38 Handoff.select :waste @13:53:43.08 Syringe tare is 16% of full scale @13:53:43.11 PinPort.in :locking @13:53:43.17 PinPort.seek :locked @13:53:43.66 Archive.in :fast @13:53:43.77 Lysis2.in :fast @13:53:43.82 Lysis1.seek 1000uL @13:53:43.86 Archive.seek :full @13:53:44.01 Lysis2.seek 1000uL @13:53:48.76 delayUntil "14:03:35.92" @14:03:35.94 Lysis1.in :DAmix @14:03:36.06 Lysis2.in :DAmix @14:03:36.68 Lysis1.seek 750uL @14:03:36.73 Lysis2.seek 750uL @14:04:11.90 delay 5 seconds @14:04:16.92 Handoff.select SPR @14:04:17.04 SPR.command "start 2 14" @14:04:17.63 SPR stabilized @14:04:17.78 delay 10 seconds @14:04:27.80 SPR.command "start 1 0" @14:04:31.52 Waiting up to 4:00 for SPR to enter state 1 @14:06:36.23 SPR.command "hold_on" @14:06:37.62 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @14:06:38.24 Lysis1.seek 450uL @14:06:38.29 Lysis2.seek 450uL @14:07:20.36 delay 15 seconds @14:07:35.38 SPR.command "hold_off" @14:07:38.27 Waiting up to 38:00 for SPR to enter state 7 @14:16:04.82 Session Begun @14:16:09.03 -> showlog @14:07:20.36 delay 15 seconds @14:07:35.38 SPR.command "hold_off" @14:07:38.27 Waiting up to 38:00 for SPR to enter state 7 @14:16:04.82 Session Begun @14:16:09.03 -> showlog @14:16:17.47 -> showlog -500 @14:25:37.89 SPRsummary:-0.738667,16.7093,21.652,29.0987 after 2006s @14:32:15.25 delay 10 seconds @14:32:25.28 SPR.command "start 1 0" @14:32:27.96 Waiting up to 4:00 for SPR to enter state 1 @14:34:32.26 SPR.command "hold_on" @14:34:33.64 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:34:34.28 Lysis1.seek 300uL @14:34:34.35 Lysis2.seek 300uL @14:34:55.49 delay 15 seconds @14:35:10.51 SPR.command "hold_off" @14:35:14.00 Waiting up to 38:00 for SPR to enter state 7 @14:59:51.41 delay 10 seconds @15:00:01.44 SPR.command "start 1 0" @15:00:04.66 Waiting up to 4:00 for SPR to enter state 1 @15:02:09.36 SPR.command "hold_on" @15:02:10.74 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:02:11.37 Lysis1.seek 150uL @15:02:11.41 Lysis2.seek 150uL @15:02:32.57 delay 15 seconds @15:02:47.59 SPR.command "hold_off" @15:02:50.58 Waiting up to 38:00 for SPR to enter state 7 @15:27:27.56 delay 10 seconds @15:27:37.59 SPR.command "start 1 0" @15:27:40.79 Waiting up to 4:00 for SPR to enter state 1 @15:29:44.57 SPR.command "hold_on" @15:29:45.95 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @15:29:46.55 Lysis1.seek 0uL @15:29:46.65 Lysis2.seek 0uL @15:30:07.78 delay 15 seconds @15:30:22.80 SPR.command "hold_off" @15:30:25.79 Waiting up to 38:00 for SPR to enter state 7 @15:55:02.66 SPR analysis completed @15:55:04.83 Ended log spr23mar2713h.spr.bz2 @15:55:04.85 SPR.command "stats_off" @15:55:05.13 Handoff.select :close @15:55:05.76
Gate.power :heat,:OFF @15:55:05.78 Gate.power :cartridge,:OFF @15:55:05.91 PinPort.in :unlocking @15:55:06.00 PinPort.seek :clear @15:55:06.51 Carousel.in :default @15:55:06.70 Carousel.home.seek 46 @15:55:07.06 Plunge.seek :locked @15:55:07.26 Archive.in :unlocking @15:55:07.44 Archive.seek :clear @15:55:07.57 Air.in :unlocking @15:55:07.74 Air.seek :clear @15:55:07.79 Lysis1.in :unlocking @15:55:08.02 Lysis1.seek :clear @15:55:08.08 Lysis2.in :unlocking @15:55:08.24 Lysis2.seek :clear @15:55:13.40
Gate.power :motor,:OFF @15:55:13.42 Gate.power :core,:OFF @15:55:13.46 Gate.power :analytic1,:OFF @15:55:13.50 Gate.power :analytic2,:OFF @15:55:13.54 Gate.power :analytic3,:OFF @15:55:13.58 Gate.power :main,:OFF @15:55:13.62 Safely stopped and ready to power off @15:55:14.07 Cartridge::None in MAIN -- Not enough standard cartridges remain @15:55:14.21 Session Ended @13:00:19.60PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:00:19.63 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:00:24.46 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:00:25.18 Sea pressure (Abs) tare is 6% of full scale @13:00:29.46 Polling Can every 2:00 @13:00:29.51 Loading /home/methane/esp2/bin/processAll mission @13:00:29.81 Sea pressure (Abs) tare is 6% of full scale @13:00:29.82 Sampling default goal volumes @13:00:29.84 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:00:29.98 Gate.power :analytic1,:ON,limit to 5000mA @13:00:30.02 Gate.power :analytic2,:ON,limit to 5000mA @13:00:30.03 Gate.power :analytic3,:ON,limit to 5000mA @13:00:30.74 Archive.configure :default @13:00:30.93 SPR Module 1, FW Rev. B, FPGA Rev. C @13:00:31.00
Air.configure :default @13:00:31.12 Lysis1.configure :default @13:00:31.26 Lysis2.configure :default @13:00:31.41 Plunge.configure :default @13:00:31.55 SamplePump.configure :default @13:00:31.70 Toroid.configure :default @13:00:31.85 Carousel.configure :default @13:00:32.00 Pressure.configure :default @13:00:32.15 ExtraPump.configure :default @13:00:32.27 PinPort.configure :default @13:00:32.33 All elf controllers running firmware version 4.83 @13:00:32.72 Archive.in :default @13:00:33.30 Toroid.in :default @13:00:33.38 Archive.home.jog 0 @13:00:33.41 Carousel.in :default @13:00:33.45 Lysis1.in :default @13:00:33.48 Air.in :default @13:00:33.53 PinPort.in :default @13:00:33.58 Plunge.in :default @13:00:33.62 Lysis2.in :default @13:00:33.98 Toroid.home.jog 0 @13:00:34.01 Lysis1.home.jog 0 @13:00:34.05 Carousel.home.jog 0 @13:00:34.07 Plunge.home.jog 0 @13:00:34.09 Air.home.jog 0 @13:00:34.12 PinPort.home.jog 0 @13:00:34.14 Lysis2.home.jog 0 @13:00:35.30 Toroid.in :homing @13:00:35.36 Plunge.in :homing @13:00:35.41 Lysis1.in :homing @13:00:35.44 Archive.in :homing @13:00:35.50 PinPort.in :homing @13:00:35.55 Lysis2.in :homing @13:00:35.98 Air.in :homing @13:00:36.36 Toroid.jog 615 @13:00:36.43 Plunge.jog 376 @13:00:36.47 PinPort.jog -109 @13:00:36.49 Lysis1.jog 1383 @13:00:36.52 Air.jog 1376 @13:00:36.55 Archive.jog 1373 @13:00:36.63 Lysis2.jog 1382 @13:00:37.00 PinPort.setRawPosition! 0 @13:00:37.04 PinPort.seek :home @13:00:37.13 PinPort.in :default @13:00:37.29 Plunge.setRawPosition! 50 @13:00:37.34 Plunge.seek :home @13:00:37.61 Plunge.in :default @13:00:37.90 Lysis1.setRawPosition! 50 @13:00:37.96 Lysis1.seek :home @13:00:38.09 Air.setRawPosition! 50 @13:00:38.12 Archive.setRawPosition! 50 @13:00:38.20 Air.seek :home @13:00:38.31 Archive.seek :home @13:00:38.44 Lysis2.setRawPosition! 50 @13:00:38.48 Lysis1.in :default @13:00:38.54 Lysis2.seek :home @13:00:38.73 Toroid.setRawPosition! 0 @13:00:38.76 Air.in :default @13:00:38.81 Archive.in :default @13:00:38.93 Toroid.seek :home @13:00:39.12 Lysis2.in :default @13:00:39.15 Toroid.in :default @13:00:39.53
Toroid.seek :clear @13:00:41.14 Carousel.in :default @13:00:41.27 Carousel.home.seek 60 @13:00:41.56 Selecting Cartridge 56 @13:00:41.87 Carousel.seek 56 @13:00:43.61 Lysis1.in :fast @13:00:43.67 Lysis1.seek :locked @13:00:46.30 Plunge.seek :locked @13:00:46.46 Air.in :fast @13:00:46.52 Air.seek :locked @13:00:48.09
Carousel.reconfigure :locked @13:00:48.25 Powering cartridge bus @13:00:50.94 Gate.setIR 1750,5000,0,6 @13:00:51.06 Gate.setIR 0,5000,0,6 @13:00:51.13 PuckHeater.configure :default @13:00:51.19 Gate.power :heat,:ON,limit to 5000mA @13:00:52.36 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:00:52.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:00:53.06 TypeError in PROCESSING -- nil can't be coerced into Float @13:00:53.14 Thread::ChildDied in MAIN -- :PROCESSING @13:00:53.18 Waiting to Resume from Checkpoint ":PROCESSING" @13:02:16.31 Session Begun @13:02:17.99 -> slot {56 => { Type: :daSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000}} @13:02:47.63 -> Cartridge.unlock @13:02:47.65 Gate.power :heat,:OFF @13:02:47.70 Gate.power :cartridge,:OFF @13:02:47.83 Carousel.in :default @13:02:48.01 Carousel.home.seek 56 @13:02:48.49 Air.in :unlocking @13:02:48.64 Air.seek :clear @13:02:50.02 Lysis1.in :unlocking @13:02:50.18 Lysis1.seek :clear @13:02:50.31 Plunge.in :unlocking @13:02:50.41 Plunge.seek :clear @13:02:57.39 -> slot {56 => { Type: :daSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000}} @13:04:37.12 -> slot 56 =>[:daSPR_phyto, :standard, {lysed:1000, lysed2:1000}] {56 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @13:04:45.13 -> slot {56 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}} @13:05:08.48 -> MainThread.exit @13:05:08.59 Intake.close @13:05:08.68 Exhaust.close @13:05:08.72 Bypass.close # @13:05:09.16 Session Ended @13:07:19.74PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:07:19.76 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:07:24.30 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:07:25.04 Sea pressure (Abs) tare is 6% of full scale @13:07:29.22 Polling Can every 2:00 @13:07:29.26 Loading /home/methane/esp2/bin/processAll mission @13:07:29.52 Sea pressure (Abs) tare is 6% of full scale @13:07:29.53 Sampling default goal volumes @13:07:29.55 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:07:29.72 Gate.power :analytic1,:ON,limit to 5000mA @13:07:29.73 Gate.power :analytic2,:ON,limit to 5000mA @13:07:29.77 Gate.power :analytic3,:ON,limit to 5000mA @13:07:30.52 Archive.configure :default @13:07:30.68 Air.configure :default @13:07:30.85 SPR Module 1, FW Rev. B, FPGA Rev. C @13:07:30.90
Lysis1.configure :default @13:07:31.03 Lysis2.configure :default @13:07:31.18 Plunge.configure :default @13:07:31.33 SamplePump.configure :default @13:07:31.49 Toroid.configure :default @13:07:31.55 Toroid.hold @13:07:31.61 Toroid.configure :default @13:07:31.77 Carousel.configure :default @13:07:31.84 Carousel.hold @13:07:31.91 Carousel.configure :default @13:07:32.06 Pressure.configure :default @13:07:32.21 ExtraPump.configure :default @13:07:32.32 PinPort.configure :default @13:07:32.39 All elf controllers running firmware version 4.83 @13:07:33.22 Toroid.seek :clear @13:07:33.30 Carousel.in :default @13:07:33.43 Carousel.home.seek 56 @13:07:33.70 Selecting Cartridge 56 @13:07:33.99 Carousel.seek 56 @13:07:35.25 Lysis1.in :fast @13:07:35.31 Lysis1.seek :locked @13:07:37.95 Plunge.seek :locked @13:07:38.11 Air.in :fast @13:07:38.17 Air.seek :locked @13:07:39.74
Carousel.reconfigure :locked @13:07:39.91 Powering cartridge bus @13:07:42.56 Gate.setIR 1750,5000,0,6 @13:07:42.68 Gate.setIR 0,5000,0,6 @13:07:42.75 PuckHeater.configure :default @13:07:42.82 Gate.power :heat,:ON,limit to 5000mA @13:07:43.59 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:07:43.62 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:07:44.16 TypeError in PROCESSING -- nil can't be coerced into Float @13:07:44.24 Thread::ChildDied in MAIN -- :PROCESSING @13:07:44.26 Waiting to Resume from Checkpoint ":PROCESSING" @13:08:51.41 Session Begun @13:08:55.96 -> trouble >>> Thread[:MAIN] is suspended <<< @13:07:29 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:07:33 Selecting Cartridge 56 @13:07:43 :PROCESSING @13:09:27.72 -> man resume @13:09:27.78 NoMethodError in bill -- undefined method `man' for CmdServer::Client:Class @13:09:27.84
Resuming Thread after :PROCESSING at 13:07:43 @13:09:28.02 Intake.close @13:09:28.10 Exhaust.close @13:09:28.23 Bypass.close @13:09:28.43
Gate.power :heat,:OFF @13:09:28.45 Gate.power :cartridge,:OFF @13:09:28.58 Carousel.in :default @13:09:28.75 Carousel.home.seek 56 @13:09:29.11 Plunge.seek :locked @13:09:29.38 Air.in :unlocking @13:09:29.57 Air.seek :clear @13:09:29.68 Lysis1.in :unlocking @13:09:29.93 Lysis1.seek :clear @13:09:32.04
Gate.power :motor,:OFF @13:09:32.06 Gate.power :core,:OFF @13:09:32.09 Gate.power :analytic1,:OFF @13:09:32.13 Gate.power :analytic2,:OFF @13:09:32.17 Gate.power :analytic3,:OFF @13:09:32.21 Gate.power :main,:OFF @13:09:32.24 Safely stopped and ready to power off @13:09:32.69 Cartridge::None in MAIN -- Not enough standard cartridges remain @13:09:32.81 Session Ended @13:17:21.58PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @13:17:21.61 >BREAK< ESP3 Gateway v1.42 for cartridge bus @13:17:26.10 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @13:17:26.82 Sea pressure (Abs) tare is 6% of full scale @13:17:31.12 Polling Can every 2:00 @13:17:31.17 Loading /home/methane/esp2/bin/processAll mission @13:17:31.45 Sea pressure (Abs) tare is 6% of full scale @13:17:31.47 Sampling default goal volumes @13:17:31.49 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @13:17:31.66 Gate.power :analytic1,:ON,limit to 5000mA @13:17:31.67 Gate.power :analytic2,:ON,limit to 5000mA @13:17:31.69 Gate.power :analytic3,:ON,limit to 5000mA @13:17:32.42 Archive.configure :default @13:17:32.60 Air.configure :default @13:17:32.72 Lysis1.configure :default @13:17:32.85 Lysis2.configure :default @13:17:33.07 SPR Module 1, FW Rev. B, FPGA Rev. C @13:17:33.10
Plunge.configure :default @13:17:33.26 SamplePump.configure :default @13:17:33.41 Toroid.configure :default @13:17:33.47 Toroid.hold @13:17:33.54 Toroid.configure :default @13:17:33.70 Carousel.configure :default @13:17:33.76 Carousel.hold @13:17:33.82 Carousel.configure :default @13:17:33.97 Pressure.configure :default @13:17:34.13 ExtraPump.configure :default @13:17:34.25 PinPort.configure :default @13:17:34.31 All elf controllers running firmware version 4.83 @13:17:35.14 Toroid.seek :clear @13:17:35.22 Carousel.in :default @13:17:35.35 Carousel.home.seek 56 @13:17:35.62 Selecting Cartridge 56 @13:17:35.92 Carousel.seek 56 @13:17:37.17 Lysis1.in :fast @13:17:37.23 Lysis1.seek :locked @13:17:39.86 Plunge.seek :locked @13:17:40.01 Air.in :fast @13:17:40.08 Air.seek :locked @13:17:41.65
Carousel.reconfigure :locked @13:17:41.81 Powering cartridge bus @13:17:44.47 Gate.setIR 1750,5000,0,6 @13:17:44.59 Gate.setIR 0,5000,0,6 @13:17:44.66 PuckHeater.configure :default @13:17:44.72 Gate.power :heat,:ON,limit to 5000mA @13:17:45.54 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @13:17:45.57 Using default cartridge heat loss curve for HeatingAccelInCarousel @13:17:46.08 TypeError in PROCESSING -- nil can't be coerced into Float @13:17:46.16 Cmd::Error in MAIN -- Not Processing @13:17:46.22 Thread::ChildDied in MAIN -- :PROCESSING @13:17:46.24 Waiting to Resume from Checkpoint ":PROCESSING" @13:27:04.72 Session Begun @13:27:12.36 -> MainThread.exit @13:27:12.48 Intake.close @13:27:12.58 Exhaust.close @13:27:12.64 Bypass.close # @13:27:13.05 Session Ended @14:21:08.97PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @14:21:09.00 >BREAK< ESP3 Gateway v1.42 for cartridge bus @14:21:13.66 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @14:21:14.39 Sea pressure (Abs) tare is 6% of full scale @14:21:18.74 Polling Can every 2:00 @14:21:18.78 Loading /home/methane/esp2/bin/processAll mission @14:21:19.12 Sea pressure (Abs) tare is 6% of full scale @14:21:19.13 Sampling default goal volumes @14:21:19.15 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @14:21:19.32 Gate.power :analytic1,:ON,limit to 5000mA @14:21:19.33 Gate.power :analytic2,:ON,limit to 5000mA @14:21:19.34 Gate.power :analytic3,:ON,limit to 5000mA @14:21:19.94 SPR Module 1, FW Rev. B, FPGA Rev. C @14:21:20.13
Archive.configure :default @14:21:20.19 Archive.hold @14:21:20.25 Archive.configure :default @14:21:20.40 Air.configure :default @14:21:20.46 Air.hold @14:21:20.54 Air.configure :default @14:21:20.66 Lysis1.configure :default @14:21:20.72 Lysis1.hold @14:21:20.79 Lysis1.configure :default @14:21:20.95 Lysis2.configure :default @14:21:21.02 Lysis2.hold @14:21:21.09 Lysis2.configure :default @14:21:21.24 Plunge.configure :default @14:21:21.30 Plunge.hold @14:21:21.37 Plunge.configure :default @14:21:21.52 SamplePump.configure :default @14:21:21.69 Toroid.configure :default @14:21:21.75 Toroid.hold @14:21:21.82 Toroid.configure :default @14:21:21.97 Carousel.configure :default @14:21:22.03 Carousel.hold @14:21:22.10 Carousel.configure :default @14:21:22.25 Pressure.configure :default @14:21:22.40 ExtraPump.configure :default @14:21:22.52 PinPort.configure :default @14:21:22.59 PinPort.hold @14:21:22.65 PinPort.configure :default @14:21:22.71 All elf controllers running firmware version 4.83 @14:21:23.51 Toroid.seek :clear @14:21:23.60 Carousel.in :default @14:21:23.72 Carousel.home.seek 56 @14:21:24.43 Selecting Cartridge 56 @14:21:24.75 Carousel.seek 56 @14:21:25.58 Lysis1.in :fast @14:21:25.64 Lysis1.seek :locked @14:21:28.27 Plunge.seek :locked @14:21:28.43 Air.in :fast @14:21:28.50 Air.seek :locked @14:21:30.07
Carousel.reconfigure :locked @14:21:30.24 Powering cartridge bus @14:21:32.92 Gate.setIR 1750,5000,0,6 @14:21:33.05 Gate.setIR 0,5000,0,6 @14:21:33.11 PuckHeater.configure :default @14:21:33.18 Gate.power :heat,:ON,limit to 5000mA @14:21:33.96 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @14:21:33.99 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:21:34.69 TypeError in PROCESSING -- nil can't be coerced into Float @14:21:34.77 Thread::ChildDied in MAIN -- :PROCESSING @14:21:34.79 Waiting to Resume from Checkpoint ":PROCESSING" @14:25:58.83PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @14:25:58.86 >BREAK< ESP3 Gateway v1.42 for cartridge bus @14:26:03.50 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @14:26:04.22 Sea pressure (Abs) tare is 6% of full scale @14:26:08.56 Polling Can every 2:00 @14:26:08.63 Loading /home/methane/esp2/bin/processAll mission @14:26:08.97 Sea pressure (Abs) tare is 6% of full scale @14:26:08.98 Sampling default goal volumes @14:26:09.00 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @14:26:09.17 Gate.power :analytic1,:ON,limit to 5000mA @14:26:09.18 Gate.power :analytic2,:ON,limit to 5000mA @14:26:09.19 Gate.power :analytic3,:ON,limit to 5000mA @14:26:09.96 SPR Module 1, FW Rev. B, FPGA Rev. C @14:26:09.99
Archive.configure :default @14:26:10.14 Air.configure :default @14:26:10.27 Lysis1.configure :default @14:26:10.39 Lysis2.configure :default @14:26:10.54 Plunge.configure :default @14:26:10.73 SamplePump.configure :default @14:26:10.89 Toroid.configure :default @14:26:10.96 Toroid.hold @14:26:11.03 Toroid.configure :default @14:26:11.18 Carousel.configure :default @14:26:11.24 Carousel.hold @14:26:11.30 Carousel.configure :default @14:26:11.46 Pressure.configure :default @14:26:11.61 ExtraPump.configure :default @14:26:11.73 PinPort.configure :default @14:26:11.81 All elf controllers running firmware version 4.83 @14:26:12.67 Toroid.seek :clear @14:26:12.75 Carousel.in :default @14:26:12.89 Carousel.home.seek 56 @14:26:13.15 Selecting Cartridge 56 @14:26:13.46 Carousel.seek 56 @14:26:14.75 Lysis1.in :fast @14:26:14.82 Lysis1.seek :locked @14:26:17.45 Plunge.seek :locked @14:26:17.61 Air.in :fast @14:26:17.68 Air.seek :locked @14:26:19.26
Carousel.reconfigure :locked @14:26:19.42 Powering cartridge bus @14:26:22.08 Gate.setIR 1750,5000,0,6 @14:26:22.20 Gate.setIR 0,5000,0,6 @14:26:22.27 PuckHeater.configure :default @14:26:22.32 Gate.power :heat,:ON,limit to 5000mA @14:26:23.12 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @14:26:23.15 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:26:23.67 TypeError in PROCESSING -- nil can't be coerced into Float @14:26:23.75 Thread::ChildDied in MAIN -- :PROCESSING @14:26:23.79 Waiting to Resume from Checkpoint ":PROCESSING" @17:02:30.46 Session Begun @17:02:40.04 -> MainThread.exit @17:02:40.17 Exhaust.close @17:02:40.26 Bypass.close @17:02:40.32 Intake.close # @17:02:40.76 Session Ended @17:04:24.42PDT30-Mar-23
>BREAK< ESP3 Gateway v1.42 for core bus @17:04:24.45 >BREAK< ESP3 Gateway v1.42 for cartridge bus @17:04:28.83 Configuration for honu by methane@mbari.org 1/24/23 /home/methane/esp2/type/auv/honu/configure.rb @17:04:29.53 Sea pressure (Abs) tare is 6% of full scale @17:04:33.68 Polling Can every 2:00 @17:04:33.74 Loading /home/methane/esp2/bin/processAll mission @17:04:34.02 Sea pressure (Abs) tare is 6% of full scale @17:04:34.04 Sampling default goal volumes @17:04:34.06 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @17:04:34.22 Gate.power :analytic1,:ON,limit to 5000mA @17:04:34.24 Gate.power :analytic2,:ON,limit to 5000mA @17:04:34.25 Gate.power :analytic3,:ON,limit to 5000mA @17:04:34.98 Archive.configure :default @17:04:35.04 Archive.hold @17:04:35.11 Archive.configure :default @17:04:35.27 Air.configure :default @17:04:35.33 Air.hold @17:04:35.40 Air.configure :default @17:04:35.56 SPR Module 1, FW Rev. B, FPGA Rev. C @17:04:35.59
Lysis1.configure :default @17:04:35.65 Lysis1.hold @17:04:35.72 Lysis1.configure :default @17:04:35.85 Lysis2.configure :default @17:04:35.91 Lysis2.hold @17:04:35.98 Lysis2.configure :default @17:04:36.14 Plunge.configure :default @17:04:36.20 Plunge.hold @17:04:36.26 Plunge.configure :default @17:04:36.41 SamplePump.configure :default @17:04:36.57 Toroid.configure :default @17:04:36.66 Toroid.hold @17:04:36.73 Toroid.configure :default @17:04:36.89 Carousel.configure :default @17:04:36.95 Carousel.hold @17:04:37.01 Carousel.configure :default @17:04:37.16 Pressure.configure :default @17:04:37.31 ExtraPump.configure :default @17:04:37.43 PinPort.configure :default @17:04:37.49 PinPort.hold @17:04:37.56 PinPort.configure :default @17:04:37.62 All elf controllers running firmware version 4.83 @17:04:38.42 Toroid.seek :clear @17:04:38.50 Carousel.in :default @17:04:39.10 Carousel.home.seek 56 @17:04:39.50 Carousel.reconfigure :locked @17:04:39.73 Gate.setIR 1750,5000,0,6 @17:04:39.86 Gate.setIR 0,5000,0,6 @17:04:39.93 PuckHeater.configure :default @17:04:39.99 Gate.power :heat,:ON,limit to 5000mA @17:04:40.81 Mounted lysis Cartridge base#L5-0009, top#L8-0084, board#Q05050 @17:04:40.85 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:04:40.89 Selecting Cartridge 56 @17:04:42.79 SPR.command "read_sequences" @17:04:43.04 SPR.command "stats_on" @17:04:43.19 SPR.command "start 0 1" @17:04:43.90 SPR.start /var/log/methane/spr23mar3017h.spr.bz2 @17:04:46.41 Waiting for baseline SPR stabilization @17:04:46.43 Handoff.select :waste @17:04:53.13 Syringe tare is 16% of full scale @17:04:53.16 PinPort.in :locking @17:04:53.22 PinPort.seek :locked @17:04:53.72 Archive.in :fast @17:04:53.79 Lysis2.in :fast @17:04:53.90 Lysis1.in :fast @17:04:54.10 Lysis2.seek 1000uL @17:04:54.15 Archive.seek :full @17:04:54.20 Lysis1.seek 1000uL @17:04:58.68 delayUntil "17:14:46.38" @17:14:46.39 Lysis2.in :DAmix @17:14:46.52 Lysis1.in :DAmix @17:14:47.09 Lysis2.seek 750uL @17:14:47.15 Lysis1.seek 750uL @17:15:22.30 delay 5 seconds @17:15:27.32 Handoff.select SPR @17:15:27.45 SPR.command "start 2 14" @17:15:28.11 SPR stabilized @17:15:28.28 delay 10 seconds @17:15:38.30 SPR.command "start 1 0" @17:15:41.99 Waiting up to 4:00 for SPR to enter state 1 @17:17:46.71 SPR.command "hold_on" @17:17:48.65 SPR Dilution 1 of 4 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @17:17:49.21 Lysis2.seek 450uL @17:17:49.27 Lysis1.seek 450uL @17:18:31.34 delay 15 seconds @17:18:46.36 SPR.command "hold_off" @17:18:49.70 Waiting up to 38:00 for SPR to enter state 7 @17:36:48.37 SPRsummary:-176RIU,133RIU,123RIU,240RIU after 2031s @17:43:25.91 delay 10 seconds @17:43:35.95 SPR.command "start 1 0" @17:43:39.39 Waiting up to 4:00 for SPR to enter state 1 @17:45:43.68 SPR.command "hold_on" @17:45:45.06 SPR Dilution 2 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:45:45.66 Lysis2.seek 300uL @17:45:45.71 Lysis1.seek 300uL @17:46:06.83 delay 15 seconds @17:46:21.85 SPR.command "hold_off" @17:46:25.43 Waiting up to 38:00 for SPR to enter state 7 @18:11:02.83 delay 10 seconds @18:11:12.86 SPR.command "start 1 0" @18:11:16.06 Waiting up to 4:00 for SPR to enter state 1 @18:13:20.77 SPR.command "hold_on" @18:13:22.17 SPR Dilution 3 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:13:22.75 Lysis2.seek 150uL @18:13:22.82 Lysis1.seek 150uL @18:13:43.93 delay 15 seconds @18:13:58.95 SPR.command "hold_off" @18:14:02.01 Waiting up to 38:00 for SPR to enter state 7 @18:38:38.99 delay 10 seconds @18:38:49.02 SPR.command "start 1 0" @18:38:51.70 Waiting up to 4:00 for SPR to enter state 1 @18:40:55.98 SPR.command "hold_on" @18:40:57.37 SPR Dilution 4 of 4 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @18:40:57.95 Lysis2.seek 0uL @18:40:58.02 Lysis1.seek 0uL @18:41:19.11 delay 15 seconds @18:41:34.13 SPR.command "hold_off" @18:41:37.22 Waiting up to 38:00 for SPR to enter state 7 @19:06:14.06 SPR analysis completed @19:06:16.13 Ended log spr23mar3017h.spr.bz2 @19:06:16.14 SPR.command "stats_off" @19:06:17.05 Intake.close @19:06:17.12 Exhaust.close @19:06:17.19 Bypass.close @19:06:17.29 Handoff.select :close @19:06:17.53
Gate.power :heat,:OFF @19:06:17.55 Gate.power :cartridge,:OFF @19:06:17.63 PinPort.in :unlocking @19:06:17.69 PinPort.seek :clear @19:06:18.16 Carousel.in :default @19:06:18.29 Carousel.home.seek 56 @19:06:18.65 Plunge.seek :locked @19:06:18.81 Archive.in :unlocking @19:06:18.96 Archive.seek :clear @19:06:19.05 Air.in :unlocking @19:06:19.20 Air.seek :clear @19:06:19.36 Lysis1.in :unlocking @19:06:19.44 Lysis1.seek :clear @19:06:19.53 Lysis2.in :unlocking @19:06:19.62 Lysis2.seek :clear @19:06:24.78
Gate.power :motor,:OFF @19:06:24.80 Gate.power :core,:OFF @19:06:24.81 Gate.power :analytic1,:OFF @19:06:24.82 Gate.power :analytic2,:OFF @19:06:24.83 Gate.power :analytic3,:OFF @19:06:24.85 Gate.power :main,:OFF @19:06:24.86 Safely stopped and ready to power off @19:06:25.33 Cartridge::None in MAIN -- Not enough standard cartridges remain