@18:43:15.07PDT20-Jul-23
Environmental Sample Processor @18:43:17.08 >BREAK< ESP3 Gateway v1.42 for core bus @18:43:17.11 >BREAK< ESP3 Gateway v1.42 for cartridge bus @18:43:21.61 Configuration for honu by nadia@mbari.org 7/20/23 /home/methane/esp2/type/auv/honu/configure.rb @18:43:22.31 Sea pressure (Abs) tare is 6% of full scale @18:43:24.84 Polling Can every 2:00 @18:43:24.88 Loading /home/methane/esp2/bin/processAll mission @18:43:25.19 Sea pressure (Abs) tare is 6% of full scale @18:43:25.21 Sampling default goal volumes @18:43:25.23 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @18:43:25.44 Gate.power :sampler,:OFF,limit to 3500mA @18:43:25.45 Gate.power :analytic1,:ON,limit to 5000mA @18:43:25.47 Gate.power :analytic2,:ON,limit to 5000mA @18:43:26.61 Archive.configure :default @18:43:26.69 Archive.hold @18:43:26.76 Archive.configure :default @18:43:26.93 Air.configure :default @18:43:26.99 Air.hold @18:43:27.07 Air.configure :default @18:43:27.21 SPR Module 1, FW Rev. B, FPGA Rev. C @18:43:27.28
Lysis1.configure :default @18:43:27.37 Lysis1.hold @18:43:27.44 Lysis1.configure :default @18:43:27.57 Lysis2.configure :default @18:43:27.63 Lysis2.hold @18:43:27.71 Lysis2.configure :default @18:43:27.86 Plunge.configure :default @18:43:27.93 Plunge.hold @18:43:28.00 Plunge.configure :default @18:43:28.16 SamplePump.configure :default @18:43:28.32 Toroid.configure :default @18:43:28.39 Toroid.hold @18:43:28.47 Toroid.configure :default @18:43:28.62 Carousel.configure :default @18:43:28.68 Carousel.hold @18:43:28.76 Carousel.configure :default @18:43:28.91 Pressure.configure :default @18:43:29.08 ExtraPump.configure :default @18:43:29.20 PinPort.configure :default @18:43:29.27 PinPort.hold @18:43:29.38 PinPort.configure :default @18:43:29.44 All elf controllers running firmware version 4.84 @18:43:30.24 Toroid.seek :clear @18:43:30.32 Carousel.in :default @18:43:30.46 Carousel.home.seek 53 @18:43:30.73 Selecting Cartridge 53 @18:43:31.05 Carousel.seek 53 @18:43:31.30 Lysis1.in :fast @18:43:31.40 Lysis1.seek :locked @18:43:34.04 Plunge.seek :locked @18:43:34.22 Air.in :fast @18:43:34.28 Air.seek :locked @18:43:36.19
Carousel.reconfigure :locked @18:43:36.51 Powering cartridge bus @18:43:39.20 Gate.setIR 1750,5000,0,6 @18:43:39.33 Gate.setIR 0,5000,0,6 @18:43:39.42 PuckHeater.configure :default @18:43:39.48 Gate.power :heat,:ON,limit to 5000mA @18:43:40.17 Mounted lysis Cartridge @18:43:40.20 WARNING: Using default puck temperature sensor calibration @18:43:40.22 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:43:41.80 SPR.command "read_sequences" @18:43:42.22 SPR.command "stats_on" @18:43:42.41 SPR.command "start 0 1" @18:43:43.09 SPR.start /var/log/methane/spr23jul2018h.spr.bz2 @18:43:45.09 Waiting for baseline SPR stabilization @18:43:45.10 Handoff.select :waste @18:43:51.81 Syringe tare is 19% of full scale @18:43:51.84 PinPort.in :locking @18:43:51.90 PinPort.seek :locked @18:43:54.44 Archive.in :fast @18:43:54.50 Lysis2.in :fast @18:43:54.55 Lysis1.seek 1000uL @18:43:54.77 Lysis2.seek 1000uL @18:43:54.82 Archive.seek :full @18:43:59.35 delayUntil "18:53:44.64" @18:44:50.88 IOError in cmdSocket -- not opened for reading @18:44:50.91 Session Begun @18:44:57.36 -> MainThread.exit # @18:44:57.82 Ended log spr23jul2018h.spr.bz2 @18:44:57.84
SPR.command "stats_off" @18:44:58.49 Intake.close @18:44:58.57 Exhaust.close @18:44:58.62 Bypass.close @18:44:58.67 Handoff.select :close @18:44:59.10 Session Ended @14:42:11.64PDT21-Jul-23
Environmental Sample Processor @14:42:13.74 >BREAK< ESP3 Gateway v1.42 for core bus @14:42:13.77 >BREAK< ESP3 Gateway v1.42 for cartridge bus @14:42:18.40 Configuration for honu by nadia@mbari.org 7/20/23 /home/methane/esp2/type/auv/honu/configure.rb @14:42:19.11 Sea pressure (Abs) tare is 6% of full scale @14:42:21.91 Polling Can every 2:00 @14:42:21.95 Loading /home/methane/esp2/bin/processAll mission @14:42:22.29 Sea pressure (Abs) tare is 6% of full scale @14:42:22.31 Sampling default goal volumes @14:42:22.32 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @14:42:22.85 Gate.power :sampler,:OFF,limit to 3500mA @14:42:22.89 Gate.power :analytic1,:ON,limit to 5000mA @14:42:22.91 Gate.power :analytic2,:ON,limit to 5000mA @14:42:23.66 Archive.configure :default @14:42:23.82 Air.configure :default @14:42:23.92 SPR Module 1, FW Rev. B, FPGA Rev. C @14:42:24.01
Lysis1.configure :default @14:42:24.15 Lysis2.configure :default @14:42:24.30 Plunge.configure :default @14:42:24.47 SamplePump.configure :default @14:42:24.62 Toroid.configure :default @14:42:24.68 Toroid.hold @14:42:24.75 Toroid.configure :default @14:42:24.91 Carousel.configure :default @14:42:24.97 Carousel.hold @14:42:25.04 Carousel.configure :default @14:42:25.22 Pressure.configure :default @14:42:25.39 ExtraPump.configure :default @14:42:25.52 PinPort.configure :default @14:42:25.58 All elf controllers running firmware version 4.84 @14:42:26.37 Toroid.seek :clear @14:42:26.48 Carousel.in :default @14:42:26.61 Carousel.home.seek 53 @14:42:26.87 Selecting Cartridge 50 @14:42:27.17 Carousel.seek 50 @14:42:28.15 Lysis1.in :fast @14:42:28.21 Lysis1.seek :locked @14:42:30.85 Plunge.seek :locked @14:42:31.01 Air.in :fast @14:42:31.07 Air.seek :locked @14:42:32.65
Carousel.reconfigure :locked @14:42:32.81 Powering cartridge bus @14:42:35.47 Gate.setIR 1750,5000,0,6 @14:42:35.59 Gate.setIR 0,5000,0,6 @14:42:35.66 PuckHeater.configure :default @14:42:35.72 Gate.power :heat,:ON,limit to 5000mA @14:42:36.43 Mounted lysis Cartridge @14:42:36.46 WARNING: Using default puck temperature sensor calibration @14:42:36.85 Using default cartridge heat loss curve for HeatingAccelInCarousel @14:42:38.55 SPR.command "read_sequences" @14:42:38.96 SPR.command "stats_on" @14:42:39.13 SPR.command "start 0 1" @14:42:39.84 SPR.start /var/log/methane/spr23jul2114h#2.spr.bz2 @14:42:41.82 Waiting for baseline SPR stabilization @14:42:41.83 Handoff.select :waste @14:42:48.54 Syringe tare is 19% of full scale @14:42:48.56 PinPort.in :locking @14:42:48.62 PinPort.seek :locked @14:42:51.17 Archive.in :fast @14:42:51.28 Lysis2.in :fast @14:42:51.32 Lysis1.seek 1000uL @14:42:51.52 Archive.seek :full @14:42:51.55 Lysis2.seek 1000uL @14:42:56.10 delayUntil "14:52:41.36" @14:52:41.38 Lysis1.in :DAmix @14:52:41.52 Lysis2.in :DAmix @14:52:42.09 Lysis1.seek 750uL @14:52:42.12 Lysis2.seek 750uL @14:53:17.25 delay 5 seconds @14:53:22.27 Handoff.select SPR @14:53:22.40 SPR.command "start 2 14" @14:53:23.09 SPR stabilized @14:53:23.29 delay 10 seconds @14:53:33.31 SPR.command "start 2 0" @14:53:36.45 Waiting up to 11:00 for SPR to enter state 1 @14:55:34.11 SPR.command "hold_on" @14:55:36.01 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @14:55:36.60 Lysis1.seek 450uL @14:55:36.64 Lysis2.seek 450uL @14:56:18.68 delay 15 seconds @14:56:33.70 SPR.command "hold_off" @14:56:37.11 Waiting up to 38:00 for SPR to enter state 7 @15:10:56.34 SPRsummary:5.39RIU,69.2RIU,132RIU,147RIU after 1874s @15:16:38.27 IOError in cmdSocket -- not opened for reading @15:16:38.29 Session Begun @15:16:40.30 -> showlog @15:16:47.74 -> showlog @15:17:05.25 -> showlog -200 @15:22:26.93 delay 10 seconds @15:22:36.95 SPR.command "start 2 0" @15:22:40.15 Waiting up to 11:00 for SPR to enter state 1 @15:24:38.24 SPR.command "hold_on" @15:24:40.15 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @15:24:40.78 Lysis1.seek 300uL @15:24:40.83 Lysis2.seek 300uL @15:25:01.97 delay 15 seconds @15:25:17.00 SPR.command "hold_off" @15:25:20.42 Waiting up to 38:00 for SPR to enter state 7 @15:51:10.23 delay 10 seconds @15:51:20.25 SPR.command "start 2 0" @15:51:22.94 Waiting up to 11:00 for SPR to enter state 1 @15:53:20.61 SPR.command "hold_on" @15:53:21.99 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @15:53:22.70 Lysis1.seek 150uL @15:53:22.76 Lysis2.seek 150uL @15:53:43.91 delay 15 seconds @15:53:58.93 SPR.command "hold_off" @15:54:01.83 Waiting up to 38:00 for SPR to enter state 7 @16:19:52.59 delay 10 seconds @16:20:02.62 SPR.command "start 2 0" @16:20:05.30 Waiting up to 11:00 for SPR to enter state 1 @16:22:02.97 SPR.command "hold_on" @16:22:04.35 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:22:05.44 Lysis1.seek 0uL @16:22:05.50 Lysis2.seek 0uL @16:22:26.65 delay 15 seconds @16:22:41.68 SPR.command "hold_off" @16:22:44.19 Waiting up to 38:00 for SPR to enter state 7 @16:35:32.19 Session Ended @16:48:34.82 SPR analysis completed @16:48:38.83 Ended log spr23jul2114h#2.spr.bz2 @16:48:38.85 SPR.command "stats_off" @16:48:39.20 Intake.close @16:48:39.29 Exhaust.close @16:48:39.40 Bypass.close @16:48:39.48 Handoff.select :close @16:48:39.71
Gate.power :heat,:OFF @16:48:39.73 Gate.power :cartridge,:OFF @16:48:39.81 PinPort.in :unlocking @16:48:39.88 PinPort.seek :clear @16:48:41.79 Carousel.in :default @16:48:41.92 Carousel.home.seek 50 @16:48:42.72 Plunge.seek :locked @16:48:42.92 Archive.in :unlocking @16:48:43.08 Archive.seek :clear @16:48:43.14 Air.in :unlocking @16:48:43.40 Air.seek :clear @16:48:43.52 Lysis1.in :unlocking @16:48:43.69 Lysis1.seek :clear @16:48:43.75 Lysis2.in :unlocking @16:48:43.87 Lysis2.seek :clear @16:48:48.47
Gate.power :motor,:OFF @16:48:48.48 Gate.power :core,:OFF @16:48:48.50 Gate.power :analytic1,:OFF @16:48:48.51 Gate.power :analytic2,:OFF @16:48:48.52 Gate.power :main,:OFF @16:48:48.54 Safely stopped and ready to power off @16:48:49.09 Gate.power :sampler,:OFF,limit to 3500mA @16:48:52.31 Gate.power :main,:ON,limit to 5000mA @16:48:52.32 Gate.power :analytic1,:ON,limit to 5000mA @16:48:52.34 Gate.power :analytic2,:ON,limit to 5000mA @16:48:52.43 Powering core bus @16:48:54.86 SPR Module 1, FW Rev. B, FPGA Rev. C @16:48:55.24
Archive.configure :default @16:48:55.34 Air.configure :default @16:48:55.40 Lysis1.configure :default @16:48:55.47 Lysis2.configure :default @16:48:55.56 Plunge.configure :default @16:48:55.66 SamplePump.configure :default @16:48:55.76 Toroid.configure :default @16:48:55.86 Carousel.configure :default @16:48:55.95 Pressure.configure :default @16:48:56.04 ExtraPump.configure :default @16:48:56.11 PinPort.configure :default @16:48:56.17 All elf controllers running firmware version 4.84 @16:48:56.19 Gate.power :motor,:ON,limit to 5000mA @16:48:56.44 Archive.home.jog 0 @16:48:57.25 PinPort.home.jog 0 @16:48:57.29 Carousel.home.jog 0 @16:48:57.34 Toroid.home.jog 0 @16:48:57.38 Plunge.home.jog 0 @16:48:57.41 Lysis1.home.jog 0 @16:48:57.45 Air.home.jog 0 @16:48:57.49 Lysis2.home.jog 0 @16:48:58.49 Archive.in :homing @16:48:58.77 Archive.jog 1370 @16:48:58.84 PinPort.in :homing @16:48:58.87 Plunge.in :homing @16:48:58.91 Lysis1.in :homing @16:48:58.96 Lysis2.in :homing @16:48:59.01 Air.in :homing @16:48:59.04 Toroid.in :homing @16:48:59.37 PinPort.jog -112 @16:48:59.87 Plunge.jog 1554 @16:49:00.00 Lysis2.jog 1393 @16:49:00.03 Lysis1.jog 1383 @16:49:00.06 Air.jog 1363 @16:49:00.14 Toroid.jog 606 @16:49:00.57 PinPort.setRawPosition! 0 @16:49:00.58 Archive.setRawPosition! 50 @16:49:00.69 PinPort.seek :home @16:49:00.73 Archive.seek :home @16:49:00.96 PinPort.in :default @16:49:01.08 Archive.in :default @16:49:01.42 Lysis2.setRawPosition! 50 @16:49:01.50 Lysis2.seek :home @16:49:01.60 Plunge.setRawPosition! 50 @16:49:01.63 Lysis1.setRawPosition! 50 @16:49:01.65 Air.setRawPosition! 50 @16:49:01.79 Plunge.seek :home @16:49:01.83 Lysis1.seek :home @16:49:01.87 Air.seek :home @16:49:02.11 Lysis2.in :default @16:49:02.22 Plunge.in :default @16:49:02.33 Lysis1.in :default @16:49:02.36 Air.in :default @16:49:02.52 Toroid.setRawPosition! 0 @16:49:02.58 Toroid.seek :home @16:49:02.67 Toroid.in :default @16:49:02.80
Toroid.seek :clear @16:49:04.41 Carousel.in :default @16:49:04.54 Carousel.home.seek 50 @16:49:04.81 Selecting Cartridge 49 @16:49:05.13 Carousel.seek 49 @16:49:05.67 Lysis1.in :fast @16:49:05.74 Lysis1.seek :locked @16:49:08.36 Plunge.seek :locked @16:49:08.53 Air.in :fast @16:49:08.59 Air.seek :locked @16:49:10.18
Carousel.reconfigure :locked @16:49:10.34 Powering cartridge bus @16:49:12.99 Gate.setIR 1750,5000,0,6 @16:49:13.11 Gate.setIR 0,5000,0,6 @16:49:13.18 PuckHeater.configure :default @16:49:13.27 Gate.power :heat,:ON,limit to 5000mA @16:49:13.91 Mounted lysis Cartridge @16:49:13.93 WARNING: Using default puck temperature sensor calibration @16:49:13.95 Using default cartridge heat loss curve for HeatingAccelInCarousel @16:49:15.48 SPR.command "read_sequences" @16:49:15.53 SPR.command "stats_on" @16:49:15.68 SPR.command "start 0 1" @16:49:16.39 SPR.start /var/log/methane/spr23jul2116h.spr.bz2 @16:49:18.83 Waiting for baseline SPR stabilization @16:49:18.85 Handoff.select :waste @16:49:25.55 Syringe tare is 19% of full scale @16:49:25.57 PinPort.in :locking @16:49:25.63 PinPort.seek :locked @16:49:28.17 Archive.in :fast @16:49:28.24 Lysis1.seek 1000uL @16:49:28.32 Lysis2.in :fast @16:49:28.49 Lysis2.seek 1000uL @16:49:28.54 Archive.seek :full @16:49:33.08 delayUntil "16:59:18.80" @16:59:18.81 Lysis1.in :DAmix @16:59:18.94 Lysis2.in :DAmix @16:59:19.50 Lysis1.seek 750uL @16:59:19.55 Lysis2.seek 750uL @16:59:54.66 delay 5 seconds @16:59:59.68 Handoff.select SPR @16:59:59.81 SPR.command "start 2 14" @17:00:00.53 SPR stabilized @17:00:00.70 delay 10 seconds @17:00:10.72 SPR.command "start 2 0" @17:00:13.90 Waiting up to 11:00 for SPR to enter state 1 @17:02:11.57 SPR.command "hold_on" @17:02:12.95 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:02:13.62 Lysis1.seek 450uL @17:02:13.68 Lysis2.seek 450uL @17:02:55.71 delay 15 seconds @17:03:10.73 SPR.command "hold_off" @17:03:14.13 Waiting up to 38:00 for SPR to enter state 7 @17:17:34.73 SPRsummary:32.7RIU,72.7RIU,137RIU,184RIU after 1793s @17:29:05.35 delay 10 seconds @17:29:15.38 SPR.command "start 2 0" @17:29:18.02 Waiting up to 11:00 for SPR to enter state 1 @17:31:15.67 SPR.command "hold_on" @17:31:17.07 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:31:17.70 Lysis1.seek 300uL @17:31:17.75 Lysis2.seek 300uL @17:31:38.86 delay 15 seconds @17:31:53.88 SPR.command "hold_off" @17:31:56.92 Waiting up to 38:00 for SPR to enter state 7 @17:57:47.68 delay 10 seconds @17:57:57.70 SPR.command "start 2 0" @17:58:00.38 Waiting up to 11:00 for SPR to enter state 1 @17:59:58.05 SPR.command "hold_on" @17:59:59.44 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:00:00.01 Lysis1.seek 150uL @18:00:00.06 Lysis2.seek 150uL @18:00:21.16 delay 15 seconds @18:00:36.19 SPR.command "hold_off" @18:00:39.28 Waiting up to 38:00 for SPR to enter state 7 @18:26:30.04 delay 10 seconds @18:26:40.06 SPR.command "start 2 0" @18:26:43.29 Waiting up to 11:00 for SPR to enter state 1 @18:28:41.36 SPR.command "hold_on" @18:28:42.75 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:28:43.34 Lysis1.seek 0uL @18:28:43.39 Lysis2.seek 0uL @18:29:04.48 delay 15 seconds @18:29:19.50 SPR.command "hold_off" @18:29:22.59 Waiting up to 38:00 for SPR to enter state 7 @18:55:13.24 SPR analysis completed @18:55:17.32 Ended log spr23jul2116h.spr.bz2 @18:55:17.34 SPR.command "stats_off" @18:55:18.04 Handoff.select :close @18:55:18.19
Gate.power :heat,:OFF @18:55:18.20 Gate.power :cartridge,:OFF @18:55:18.29 PinPort.in :unlocking @18:55:18.35 PinPort.seek :clear @18:55:20.27 Carousel.in :default @18:55:20.40 Carousel.home.seek 49 @18:55:20.73 Plunge.seek :locked @18:55:20.90 Archive.in :unlocking @18:55:21.08 Archive.seek :clear @18:55:21.20 Air.in :unlocking @18:55:21.40 Air.seek :clear @18:55:21.45 Lysis1.in :unlocking @18:55:21.65 Lysis1.seek :clear @18:55:21.72 Lysis2.in :unlocking @18:55:21.83 Lysis2.seek :clear @18:55:26.44
Gate.power :motor,:OFF @18:55:26.46 Gate.power :core,:OFF @18:55:26.47 Gate.power :analytic1,:OFF @18:55:26.48 Gate.power :analytic2,:OFF @18:55:26.50 Gate.power :main,:OFF @18:55:26.51 Safely stopped and ready to power off @18:55:26.96 Gate.power :sampler,:OFF,limit to 3500mA @18:55:30.28 Gate.power :main,:ON,limit to 5000mA @18:55:30.30 Gate.power :analytic1,:ON,limit to 5000mA @18:55:30.31 Gate.power :analytic2,:ON,limit to 5000mA @18:55:30.44 Powering core bus @18:55:32.74 SPR Module 1, FW Rev. B, FPGA Rev. C @18:55:33.24
Archive.configure :default @18:55:33.34 Air.configure :default @18:55:33.41 Lysis1.configure :default @18:55:33.47 Lysis2.configure :default @18:55:33.57 Plunge.configure :default @18:55:33.66 SamplePump.configure :default @18:55:33.75 Toroid.configure :default @18:55:33.85 Carousel.configure :default @18:55:33.94 Pressure.configure :default @18:55:34.04 ExtraPump.configure :default @18:55:34.10 PinPort.configure :default @18:55:34.16 All elf controllers running firmware version 4.84 @18:55:34.18 Gate.power :motor,:ON,limit to 5000mA @18:55:34.56 Archive.home.jog 0 @18:55:35.30 PinPort.home.jog 0 @18:55:35.33 Plunge.home.jog 0 @18:55:35.36 Air.home.jog 0 @18:55:35.40 Toroid.home.jog 0 @18:55:35.44 Lysis2.home.jog 0 @18:55:35.47 Lysis1.home.jog 0 @18:55:35.51 Carousel.home.jog 0 @18:55:36.44 Archive.in :homing @18:55:37.25 Archive.jog 1370 @18:55:37.34 PinPort.in :homing @18:55:37.38 Plunge.in :homing @18:55:37.42 Lysis1.in :homing @18:55:37.49 Lysis2.in :homing @18:55:37.52 Toroid.in :homing @18:55:37.59 Air.in :homing @18:55:37.89 PinPort.jog -111 @18:55:37.94 Plunge.jog 1550 @18:55:37.96 Lysis1.jog 1382 @18:55:37.99 Toroid.jog 608 @18:55:38.03 Lysis2.jog 1394 @18:55:38.09 Air.jog 1364 @18:55:38.51 PinPort.setRawPosition! 0 @18:55:38.57 PinPort.seek :home @18:55:38.67 PinPort.in :default @18:55:38.77 Archive.setRawPosition! 50 @18:55:38.83 Archive.seek :home @18:55:39.11 Archive.in :default @18:55:39.39 Lysis1.setRawPosition! 50 @18:55:39.48 Plunge.setRawPosition! 50 @18:55:39.51 Lysis1.seek :home @18:55:39.61 Plunge.seek :home @18:55:39.73 Lysis2.setRawPosition! 50 @18:55:39.75 Air.setRawPosition! 50 @18:55:39.86 Lysis2.seek :home @18:55:39.89 Air.seek :home @18:55:40.08 Plunge.in :default @18:55:40.10 Lysis1.in :default @18:55:40.29 Air.in :default @18:55:40.31 Lysis2.in :default @18:55:40.46 Toroid.setRawPosition! 0 @18:55:40.52 Toroid.seek :home @18:55:40.60 Toroid.in :default @18:55:40.71
Toroid.seek :clear @18:55:42.32 Carousel.in :default @18:55:42.46 Carousel.home.seek 49 @18:55:42.71 Selecting Cartridge 48 @18:55:43.05 Carousel.seek 48 @18:55:43.59 Lysis1.in :fast @18:55:43.65 Lysis1.seek :locked @18:55:46.27 Plunge.seek :locked @18:55:46.44 Air.in :fast @18:55:46.50 Air.seek :locked @18:55:48.09
Carousel.reconfigure :locked @18:55:48.25 Powering cartridge bus @18:55:50.90 Gate.setIR 1750,5000,0,6 @18:55:51.03 Gate.setIR 0,5000,0,6 @18:55:51.10 PuckHeater.configure :default @18:55:51.16 Gate.power :heat,:ON,limit to 5000mA @18:55:51.79 Mounted lysis Cartridge @18:55:51.82 WARNING: Using default puck temperature sensor calibration @18:55:51.83 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:55:53.92 SPR.command "read_sequences" @18:55:54.37 SPR.command "stats_on" @18:55:54.55 SPR.command "start 0 1" @18:55:55.24 SPR.start /var/log/methane/spr23jul2118h.spr.bz2 @18:55:57.24 Waiting for baseline SPR stabilization @18:55:57.27 Handoff.select :waste @18:56:03.98 Syringe tare is 19% of full scale @18:56:04.00 PinPort.in :locking @18:56:04.07 PinPort.seek :locked @18:56:06.61 Archive.in :fast @18:56:06.68 Lysis2.in :fast @18:56:06.71 Lysis1.seek 1000uL @18:56:06.92 Archive.seek :full @18:56:06.96 Lysis2.seek 1000uL @18:56:11.50 delayUntil "19:05:56.79" @19:05:56.81 Lysis1.in :DAmix @19:05:56.94 Lysis2.in :DAmix @19:05:57.51 Lysis1.seek 750uL @19:05:57.56 Lysis2.seek 750uL @19:06:32.70 delay 5 seconds @19:06:37.72 Handoff.select SPR @19:06:37.85 SPR.command "start 2 14" @19:06:38.49 SPR stabilized @19:06:38.66 delay 10 seconds @19:06:48.68 SPR.command "start 2 0" @19:06:51.87 Waiting up to 11:00 for SPR to enter state 1 @19:08:49.54 SPR.command "hold_on" @19:08:51.45 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:08:52.04 Lysis1.seek 450uL @19:08:52.08 Lysis2.seek 450uL @19:09:34.14 delay 15 seconds @19:09:49.16 SPR.command "hold_off" @19:09:53.05 Waiting up to 38:00 for SPR to enter state 7 @19:24:12.70 SPRsummary:31.4RIU,74.1RIU,139RIU,183RIU after 1792s @19:35:43.31 delay 10 seconds @19:35:53.34 SPR.command "start 2 0" @19:35:55.99 Waiting up to 11:00 for SPR to enter state 1 @19:37:53.66 SPR.command "hold_on" @19:37:55.04 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:37:55.67 Lysis1.seek 300uL @19:37:55.73 Lysis2.seek 300uL @19:38:16.85 delay 15 seconds @19:38:31.87 SPR.command "hold_off" @19:38:34.89 Waiting up to 38:00 for SPR to enter state 7 @20:04:26.08 delay 10 seconds @20:04:36.10 SPR.command "start 2 0" @20:04:39.30 Waiting up to 11:00 for SPR to enter state 1 @20:06:36.96 SPR.command "hold_on" @20:06:38.35 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:06:38.91 Lysis1.seek 150uL @20:06:38.98 Lysis2.seek 150uL @20:07:00.07 delay 15 seconds @20:07:15.09 SPR.command "hold_off" @20:07:18.20 Waiting up to 38:00 for SPR to enter state 7 @20:33:09.46 delay 10 seconds @20:33:19.48 SPR.command "start 2 0" @20:33:22.61 Waiting up to 11:00 for SPR to enter state 1 @20:35:20.27 SPR.command "hold_on" @20:35:21.66 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:35:22.24 Lysis1.seek 0uL @20:35:22.29 Lysis2.seek 0uL @20:35:43.40 delay 15 seconds @20:35:58.42 SPR.command "hold_off" @20:36:01.51 Waiting up to 38:00 for SPR to enter state 7 @21:01:52.12 SPR analysis completed @21:01:56.25 Ended log spr23jul2118h.spr.bz2 @21:01:56.27 SPR.command "stats_off" @21:01:57.02 Handoff.select :close @21:01:57.17
Gate.power :heat,:OFF @21:01:57.18 Gate.power :cartridge,:OFF @21:01:57.30 PinPort.in :unlocking @21:01:57.37 PinPort.seek :clear @21:01:59.28 Carousel.in :default @21:01:59.41 Carousel.home.seek 48 @21:01:59.74 Plunge.seek :locked @21:01:59.91 Archive.in :unlocking @21:02:00.07 Archive.seek :clear @21:02:00.15 Air.in :unlocking @21:02:00.39 Air.seek :clear @21:02:00.44 Lysis1.in :unlocking @21:02:00.65 Lysis1.seek :clear @21:02:00.73 Lysis2.in :unlocking @21:02:00.83 Lysis2.seek :clear @21:02:05.43
Gate.power :motor,:OFF @21:02:05.45 Gate.power :core,:OFF @21:02:05.46 Gate.power :analytic1,:OFF @21:02:05.48 Gate.power :analytic2,:OFF @21:02:05.49 Gate.power :main,:OFF @21:02:05.50 Safely stopped and ready to power off @21:02:05.70 Gate.power :sampler,:OFF,limit to 3500mA @21:02:09.27 Gate.power :main,:ON,limit to 5000mA @21:02:09.29 Gate.power :analytic1,:ON,limit to 5000mA @21:02:09.31 Gate.power :analytic2,:ON,limit to 5000mA @21:02:09.43 Powering core bus @21:02:11.74 SPR Module 1, FW Rev. B, FPGA Rev. C @21:02:12.21
Archive.configure :default @21:02:12.30 Air.configure :default @21:02:12.37 Lysis1.configure :default @21:02:12.44 Lysis2.configure :default @21:02:12.53 Plunge.configure :default @21:02:12.63 SamplePump.configure :default @21:02:12.72 Toroid.configure :default @21:02:12.81 Carousel.configure :default @21:02:12.90 Pressure.configure :default @21:02:13.00 ExtraPump.configure :default @21:02:13.06 PinPort.configure :default @21:02:13.12 All elf controllers running firmware version 4.84 @21:02:13.14 Gate.power :motor,:ON,limit to 5000mA @21:02:13.57 Archive.home.jog 0 @21:02:14.23 Carousel.home.jog 0 @21:02:14.29 Plunge.home.jog 0 @21:02:14.32 Lysis1.home.jog 0 @21:02:14.35 Air.home.jog 0 @21:02:14.38 Toroid.home.jog 0 @21:02:14.42 Lysis2.home.jog 0 @21:02:14.45 PinPort.home.jog 0 @21:02:15.79 Archive.in :homing @21:02:16.09 Archive.jog 1371 @21:02:16.35 PinPort.in :homing @21:02:16.39 Toroid.in :homing @21:02:16.43 Lysis2.in :homing @21:02:16.47 Air.in :homing @21:02:16.51 Plunge.in :homing @21:02:16.54 Lysis1.in :homing @21:02:16.83 PinPort.jog -111 @21:02:16.88 Toroid.jog 606 @21:02:16.92 Plunge.jog 1554 @21:02:16.96 Lysis2.jog 1393 @21:02:17.00 Air.jog 1366 @21:02:17.13 Lysis1.jog 1382 @21:02:17.55 Archive.setRawPosition! 50 @21:02:17.57 PinPort.setRawPosition! 0 @21:02:17.67 PinPort.seek :home @21:02:17.70 Archive.seek :home @21:02:17.91 PinPort.in :default @21:02:18.07 Archive.in :default @21:02:18.52 Plunge.setRawPosition! 50 @21:02:18.56 Lysis2.setRawPosition! 50 @21:02:18.59 Air.setRawPosition! 50 @21:02:18.61 Lysis1.setRawPosition! 50 @21:02:18.75 Plunge.seek :home @21:02:18.79 Lysis2.seek :home @21:02:18.93 Lysis1.seek :home @21:02:18.99 Air.seek :home @21:02:19.26 Toroid.setRawPosition! 0 @21:02:19.31 Plunge.in :default @21:02:19.42 Toroid.seek :home @21:02:19.57 Lysis2.in :default @21:02:19.61 Lysis1.in :default @21:02:19.64 Toroid.in :default @21:02:19.69 Air.in :default @21:02:20.16
Toroid.seek :clear @21:02:21.77 Carousel.in :default @21:02:21.92 Carousel.home.seek 48 @21:02:22.19 Selecting Cartridge 47 @21:02:22.50 Carousel.seek 47 @21:02:23.04 Lysis1.in :fast @21:02:23.10 Lysis1.seek :locked @21:02:25.72 Plunge.seek :locked @21:02:25.88 Air.in :fast @21:02:26.43 Air.seek :locked @21:02:28.00
Carousel.reconfigure :locked @21:02:28.17 Powering cartridge bus @21:02:30.82 Gate.setIR 1750,5000,0,6 @21:02:30.94 Gate.setIR 0,5000,0,6 @21:02:31.01 PuckHeater.configure :default @21:02:31.07 Gate.power :heat,:ON,limit to 5000mA @21:02:31.70 Mounted lysis Cartridge @21:02:31.73 WARNING: Using default puck temperature sensor calibration @21:02:31.75 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:02:33.34 SPR.command "read_sequences" @21:02:33.40 SPR.command "stats_on" @21:02:33.56 SPR.command "start 0 1" @21:02:34.27 SPR.start /var/log/methane/spr23jul2121h.spr.bz2 @21:02:36.21 Waiting for baseline SPR stabilization @21:02:36.24 Handoff.select :waste @21:02:42.94 Syringe tare is 20% of full scale @21:02:42.97 PinPort.in :locking @21:02:43.04 PinPort.seek :locked @21:02:45.57 Archive.in :fast @21:02:45.64 Lysis2.in :fast @21:02:45.73 Lysis1.seek 1000uL @21:02:45.85 Archive.seek :full @21:02:45.99 Lysis2.seek 1000uL @21:02:50.52 delayUntil "21:12:35.79" @21:12:35.81 Lysis1.in :DAmix @21:12:35.94 Lysis2.in :DAmix @21:12:36.52 Lysis1.seek 750uL @21:12:36.62 Lysis2.seek 750uL @21:13:11.67 delay 5 seconds @21:13:16.69 Handoff.select SPR @21:13:16.81 SPR.command "start 2 14" @21:13:17.50 SPR stabilized @21:13:17.66 delay 10 seconds @21:13:27.69 SPR.command "start 2 0" @21:13:30.86 Waiting up to 11:00 for SPR to enter state 1 @21:15:28.54 SPR.command "hold_on" @21:15:29.92 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:15:30.55 Lysis1.seek 450uL @21:15:30.60 Lysis2.seek 450uL @21:16:12.65 delay 15 seconds @21:16:27.67 SPR.command "hold_off" @21:16:30.57 Waiting up to 38:00 for SPR to enter state 7 @21:30:50.75 SPRsummary:38RIU,74RIU,138RIU,191RIU after 1791s @21:42:21.36 delay 10 seconds @21:42:31.38 SPR.command "start 2 0" @21:42:34.04 Waiting up to 11:00 for SPR to enter state 1 @21:44:31.71 SPR.command "hold_on" @21:44:33.09 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:44:33.71 Lysis1.seek 300uL @21:44:33.80 Lysis2.seek 300uL @21:44:54.87 delay 15 seconds @21:45:09.89 SPR.command "hold_off" @21:45:12.94 Waiting up to 38:00 for SPR to enter state 7 @22:11:03.69 delay 10 seconds @22:11:13.72 SPR.command "start 2 0" @22:11:16.40 Waiting up to 11:00 for SPR to enter state 1 @22:13:14.07 SPR.command "hold_on" @22:13:15.46 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:13:16.02 Lysis1.seek 150uL @22:13:16.08 Lysis2.seek 150uL @22:13:37.19 delay 15 seconds @22:13:52.23 SPR.command "hold_off" @22:13:55.29 Waiting up to 38:00 for SPR to enter state 7 @22:38:33.28 IOError in cmdSocket -- not opened for reading @22:38:33.31 Session Begun @22:38:45.72 -> SP[:sample] acceleration=100 deceleration=9000 stopRate=9000 maxSpeed=3500 minSpeed=4 maxPWM=50 minPWM=0 maxSettling=150 maxCurrent=0 stiction=0 friction=-1 hunt=false brake=false maxDeltaPress=false maxInPress=0 maxOutPress=64 minDeltaPress=false minInPress=0 minOutPress=320 pressBias=0 pressGain=3716 inputDeltaPress=false externalCurrent=false debug=false> @22:39:32.19 -> exit @22:39:32.22 Session Ended @22:39:37.03 IOError in cmdSocket -- not opened for reading @22:39:37.05 Session Begun @22:39:39.27 -> showlog 200 @22:39:46.55 delay 10 seconds @22:39:56.57 SPR.command "start 2 0" @22:39:59.71 Waiting up to 11:00 for SPR to enter state 1 @22:40:51.24 -> threads [#, #, #, #, #, #] @22:40:57.25 -> quit @22:40:57.28 Session Ended @22:41:57.38 SPR.command "hold_on" @22:41:58.77 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:41:59.38 Lysis1.seek 0uL @22:41:59.44 Lysis2.seek 0uL @22:42:20.54 delay 15 seconds @22:42:35.57 SPR.command "hold_off" @22:42:38.61 Waiting up to 38:00 for SPR to enter state 7 @22:58:56.91 IOError in cmdSocket -- not opened for reading @22:58:57.00 Session Begun @22:59:09.08 -> Log::History # @23:06:48.96 -> exit @23:06:48.98 Session Ended @23:08:29.26 SPR analysis completed @23:08:33.27 Ended log spr23jul2121h.spr.bz2 @23:08:33.29 SPR.command "stats_off" @23:08:33.62 Handoff.select :close @23:08:33.77
Gate.power :heat,:OFF @23:08:33.78 Gate.power :cartridge,:OFF @23:08:34.30 PinPort.in :unlocking @23:08:34.38 PinPort.seek :clear @23:08:36.30 Carousel.in :default @23:08:36.44 Carousel.home.seek 47 @23:08:36.78 Plunge.seek :locked @23:08:36.95 Archive.in :unlocking @23:08:37.13 Archive.seek :clear @23:08:37.25 Air.in :unlocking @23:08:37.45 Air.seek :clear @23:08:37.51 Lysis1.in :unlocking @23:08:37.70 Lysis1.seek :clear @23:08:37.82 Lysis2.in :unlocking @23:08:37.91 Lysis2.seek :clear @23:08:42.51
Gate.power :motor,:OFF @23:08:42.53 Gate.power :core,:OFF @23:08:42.54 Gate.power :analytic1,:OFF @23:08:42.55 Gate.power :analytic2,:OFF @23:08:42.57 Gate.power :main,:OFF @23:08:42.58 Safely stopped and ready to power off @23:08:43.01 Gate.power :sampler,:OFF,limit to 3500mA @23:08:46.35 Gate.power :main,:ON,limit to 5000mA @23:08:46.37 Gate.power :analytic1,:ON,limit to 5000mA @23:08:46.38 Gate.power :analytic2,:ON,limit to 5000mA @23:08:46.51 Powering core bus @23:08:48.82 SPR Module 1, FW Rev. B, FPGA Rev. C @23:08:49.32
Archive.configure :default @23:08:49.41 Air.configure :default @23:08:49.48 Lysis1.configure :default @23:08:49.54 Lysis2.configure :default @23:08:49.64 Plunge.configure :default @23:08:49.73 SamplePump.configure :default @23:08:49.82 Toroid.configure :default @23:08:49.92 Carousel.configure :default @23:08:50.01 Pressure.configure :default @23:08:50.10 ExtraPump.configure :default @23:08:50.16 PinPort.configure :default @23:08:50.23 All elf controllers running firmware version 4.84 @23:08:50.25 Gate.power :motor,:ON,limit to 5000mA @23:08:50.62 Archive.home.jog 0 @23:08:51.29 Carousel.home.jog 0 @23:08:51.34 Plunge.home.jog 0 @23:08:51.37 Lysis1.home.jog 0 @23:08:51.40 Toroid.home.jog 0 @23:08:51.43 Lysis2.home.jog 0 @23:08:51.48 Air.home.jog 0 @23:08:51.51 PinPort.home.jog 0 @23:08:52.85 Archive.in :homing @23:08:53.29 Archive.jog 1368 @23:08:53.38 Plunge.in :homing @23:08:53.40 Lysis1.in :homing @23:08:53.45 Toroid.in :homing @23:08:53.50 Air.in :homing @23:08:53.54 PinPort.in :homing @23:08:53.59 Lysis2.in :homing @23:08:53.89 Plunge.jog 1555 @23:08:53.95 Lysis1.jog 1382 @23:08:54.06 PinPort.jog -111 @23:08:54.09 Lysis2.jog 1393 @23:08:54.13 Air.jog 1367 @23:08:54.21 Toroid.jog 603 @23:08:54.56 PinPort.setRawPosition! 0 @23:08:54.62 PinPort.seek :home @23:08:54.73 PinPort.in :default @23:08:54.83 Archive.setRawPosition! 50 @23:08:54.89 Archive.seek :home @23:08:55.17 Archive.in :default @23:08:55.36 Plunge.setRawPosition! 50 @23:08:55.44 Lysis1.setRawPosition! 50 @23:08:55.46 Plunge.seek :home @23:08:55.60 Lysis1.seek :home @23:08:55.69 Lysis2.setRawPosition! 50 @23:08:55.73 Air.setRawPosition! 50 @23:08:55.82 Lysis2.seek :home @23:08:55.87 Air.seek :home @23:08:56.04 Plunge.in :default @23:08:56.07 Lysis1.in :default @23:08:56.25 Air.in :default @23:08:56.27 Lysis2.in :default @23:08:56.50 Toroid.setRawPosition! 0 @23:08:56.55 Toroid.seek :home @23:08:56.64 Toroid.in :default @23:08:56.74
Toroid.seek :clear @23:08:58.35 Carousel.in :default @23:08:58.48 Carousel.home.seek 47 @23:08:58.75 Selecting Cartridge 46 @23:08:59.07 Carousel.seek 46 @23:08:59.62 Lysis1.in :fast @23:08:59.68 Lysis1.seek :locked @23:09:02.31 Plunge.seek :locked @23:09:02.48 Air.in :fast @23:09:02.56 Air.seek :locked @23:09:04.24
Carousel.reconfigure :locked @23:09:04.41 Powering cartridge bus @23:09:07.07 Gate.setIR 1750,5000,0,6 @23:09:07.21 Gate.setIR 0,5000,0,6 @23:09:07.30 PuckHeater.configure :default @23:09:07.36 Gate.power :heat,:ON,limit to 5000mA @23:09:08.00 Mounted lysis Cartridge @23:09:08.02 WARNING: Using default puck temperature sensor calibration @23:09:08.04 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:09:09.60 SPR.command "read_sequences" @23:09:09.66 SPR.command "stats_on" @23:09:10.58 SPR.command "start 0 1" @23:09:11.26 SPR.start /var/log/methane/spr23jul2123h.spr.bz2 @23:09:13.82 Waiting for baseline SPR stabilization @23:09:13.84 Handoff.select :waste @23:09:20.54 Syringe tare is 20% of full scale @23:09:20.57 PinPort.in :locking @23:09:20.63 PinPort.seek :locked @23:09:23.17 Archive.in :fast @23:09:23.27 Lysis2.in :fast @23:09:23.35 Lysis1.seek 1000uL @23:09:23.57 Archive.seek :full @23:09:23.60 Lysis2.seek 1000uL @23:09:28.13 delayUntil "23:19:13.79" @23:19:13.80 Lysis1.in :DAmix @23:19:13.94 Lysis2.in :DAmix @23:19:14.53 Lysis1.seek 750uL @23:19:14.62 Lysis2.seek 750uL @23:19:49.69 delay 5 seconds @23:19:54.71 Handoff.select SPR @23:19:54.84 SPR.command "start 2 14" @23:19:55.52 SPR stabilized @23:19:55.68 delay 10 seconds @23:20:05.70 SPR.command "start 2 0" @23:20:09.41 Waiting up to 11:00 for SPR to enter state 1 @23:22:07.50 SPR.command "hold_on" @23:22:09.41 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:22:10.00 Lysis1.seek 450uL @23:22:10.10 Lysis2.seek 450uL @23:22:52.13 delay 15 seconds @23:23:07.15 SPR.command "hold_off" @23:23:10.49 Waiting up to 38:00 for SPR to enter state 7 @23:37:29.72 SPRsummary:35.7RIU,67.1RIU,126RIU,179RIU after 1793s @23:49:00.30 delay 10 seconds @23:49:10.32 SPR.command "start 2 0" @23:49:13.01 Waiting up to 11:00 for SPR to enter state 1 @23:51:10.68 SPR.command "hold_on" @23:51:12.06 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:51:12.69 Lysis1.seek 300uL @23:51:12.73 Lysis2.seek 300uL @23:51:33.83 delay 15 seconds @23:51:48.85 SPR.command "hold_off" @23:51:51.90 Waiting up to 38:00 for SPR to enter state 7 @00:17:42.67PDT22-Jul-23 delay 10 seconds @00:17:52.69 SPR.command "start 2 0" @00:17:55.37 Waiting up to 11:00 for SPR to enter state 1 @00:19:53.04 SPR.command "hold_on" @00:19:54.95 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:19:55.54 Lysis1.seek 150uL @00:19:55.57 Lysis2.seek 150uL @00:20:16.71 delay 15 seconds @00:20:31.73 SPR.command "hold_off" @00:20:34.79 Waiting up to 38:00 for SPR to enter state 7 @00:46:25.03 delay 10 seconds @00:46:35.54 SPR.command "start 2 0" @00:46:39.20 Waiting up to 11:00 for SPR to enter state 1 @00:48:37.28 SPR.command "hold_on" @00:48:38.67 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:48:39.30 Lysis1.seek 0uL @00:48:39.34 Lysis2.seek 0uL @00:49:00.45 delay 15 seconds @00:49:15.47 SPR.command "hold_off" @00:49:19.04 Waiting up to 38:00 for SPR to enter state 7 @01:15:10.09 SPR analysis completed @01:15:14.14 Ended log spr23jul2123h.spr.bz2 @01:15:14.15 SPR.command "stats_off" @01:15:15.14 Handoff.select :close @01:15:15.76
Gate.power :heat,:OFF @01:15:15.77 Gate.power :cartridge,:OFF @01:15:15.86 PinPort.in :unlocking @01:15:15.92 PinPort.seek :clear @01:15:17.84 Carousel.in :default @01:15:17.97 Carousel.home.seek 46 @01:15:18.30 Plunge.seek :locked @01:15:18.47 Archive.in :unlocking @01:15:18.64 Archive.seek :clear @01:15:18.70 Air.in :unlocking @01:15:18.88 Air.seek :clear @01:15:18.99 Lysis1.in :unlocking @01:15:19.17 Lysis1.seek :clear @01:15:19.28 Lysis2.in :unlocking @01:15:19.37 Lysis2.seek :clear @01:15:23.97
Gate.power :motor,:OFF @01:15:23.99 Gate.power :core,:OFF @01:15:24.00 Gate.power :analytic1,:OFF @01:15:24.01 Gate.power :analytic2,:OFF @01:15:24.03 Gate.power :main,:OFF @01:15:24.04 Safely stopped and ready to power off @01:15:24.23 Gate.power :sampler,:OFF,limit to 3500mA @01:15:27.81 Gate.power :main,:ON,limit to 5000mA @01:15:27.83 Gate.power :analytic1,:ON,limit to 5000mA @01:15:27.84 Gate.power :analytic2,:ON,limit to 5000mA @01:15:27.96 Powering core bus @01:15:30.28 SPR Module 1, FW Rev. B, FPGA Rev. C @01:15:30.74
Archive.configure :default @01:15:30.84 Air.configure :default @01:15:30.91 Lysis1.configure :default @01:15:30.97 Lysis2.configure :default @01:15:31.07 Plunge.configure :default @01:15:31.16 SamplePump.configure :default @01:15:31.28 Toroid.configure :default @01:15:31.38 Carousel.configure :default @01:15:31.47 Pressure.configure :default @01:15:31.56 ExtraPump.configure :default @01:15:31.64 PinPort.configure :default @01:15:31.70 All elf controllers running firmware version 4.84 @01:15:31.72 Gate.power :motor,:ON,limit to 5000mA @01:15:31.93 Archive.home.jog 0 @01:15:32.72 PinPort.home.jog 0 @01:15:32.76 Carousel.home.jog 0 @01:15:32.80 Plunge.home.jog 0 @01:15:32.83 Lysis1.home.jog 0 @01:15:32.86 Air.home.jog 0 @01:15:32.89 Toroid.home.jog 0 @01:15:32.93 Lysis2.home.jog 0 @01:15:34.41 Archive.in :homing @01:15:34.70 Archive.jog 1371 @01:15:34.78 Toroid.in :homing @01:15:34.80 Lysis2.in :homing @01:15:34.84 Air.in :homing @01:15:34.88 PinPort.in :homing @01:15:34.93 Plunge.in :homing @01:15:34.97 Lysis1.in :homing @01:15:35.35 Toroid.jog 601 @01:15:35.41 Plunge.jog 1556 @01:15:35.46 Lysis2.jog 1393 @01:15:35.48 Air.jog 1368 @01:15:35.51 Lysis1.jog 1382 @01:15:35.57 PinPort.jog -111 @01:15:36.10 PinPort.setRawPosition! 0 @01:15:36.15 PinPort.seek :home @01:15:36.24 Archive.setRawPosition! 50 @01:15:36.30 Archive.seek :home @01:15:36.38 PinPort.in :default @01:15:36.58 Archive.in :default @01:15:36.91 Plunge.setRawPosition! 50 @01:15:37.01 Lysis2.setRawPosition! 50 @01:15:37.04 Air.setRawPosition! 50 @01:15:37.08 Plunge.seek :home @01:15:37.16 Lysis2.seek :home @01:15:37.29 Air.seek :home @01:15:37.48 Lysis1.setRawPosition! 50 @01:15:37.57 Plunge.in :default @01:15:37.59 Lysis1.seek :home @01:15:37.80 Toroid.setRawPosition! 0 @01:15:37.85 Air.in :default @01:15:37.87 Lysis2.in :default @01:15:38.04 Toroid.seek :home @01:15:38.15 Lysis1.in :default @01:15:38.24 Toroid.in :default @01:15:38.48
Toroid.seek :clear @01:15:40.09 Carousel.in :default @01:15:40.22 Carousel.home.seek 46 @01:15:40.50 Selecting Cartridge 45 @01:15:40.81 Carousel.seek 45 @01:15:41.37 Lysis1.in :fast @01:15:41.44 Lysis1.seek :locked @01:15:44.06 Plunge.seek :locked @01:15:44.23 Air.in :fast @01:15:44.78 Air.seek :locked @01:15:46.36
Carousel.reconfigure :locked @01:15:46.53 Powering cartridge bus @01:15:49.18 Gate.setIR 1750,5000,0,6 @01:15:49.31 Gate.setIR 0,5000,0,6 @01:15:49.37 PuckHeater.configure :default @01:15:49.43 Gate.power :heat,:ON,limit to 5000mA @01:15:50.07 Mounted lysis Cartridge @01:15:50.09 WARNING: Using default puck temperature sensor calibration @01:15:50.11 Using default cartridge heat loss curve for HeatingAccelInCarousel @01:15:51.67 SPR.command "read_sequences" @01:15:51.90 SPR.command "stats_on" @01:15:52.07 SPR.command "start 0 1" @01:15:52.77 SPR.start /var/log/methane/spr23jul2201h.spr.bz2 @01:15:54.76 Waiting for baseline SPR stabilization @01:15:54.77 Handoff.select :waste @01:16:01.48 Syringe tare is 20% of full scale @01:16:01.50 PinPort.in :locking @01:16:01.57 PinPort.seek :locked @01:16:04.11 Archive.in :fast @01:16:04.20 Lysis2.in :fast @01:16:04.27 Archive.seek :full @01:16:04.34 Lysis1.seek 1000uL @01:16:04.46 Lysis2.seek 1000uL @01:16:09.01 delayUntil "01:25:54.32" @01:25:54.34 Lysis1.in :DAmix @01:25:54.47 Lysis2.in :DAmix @01:25:55.03 Lysis1.seek 750uL @01:25:55.10 Lysis2.seek 750uL @01:26:30.24 delay 5 seconds @01:26:35.26 Handoff.select SPR @01:26:35.39 SPR.command "start 2 14" @01:26:36.55 SPR stabilized @01:26:36.71 delay 10 seconds @01:26:46.74 SPR.command "start 2 0" @01:26:49.92 Waiting up to 11:00 for SPR to enter state 1 @01:28:48.01 SPR.command "hold_on" @01:28:49.40 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @01:28:50.01 Lysis1.seek 450uL @01:28:50.07 Lysis2.seek 450uL @01:29:32.12 delay 15 seconds @01:29:47.14 SPR.command "hold_off" @01:29:50.05 Waiting up to 38:00 for SPR to enter state 7 @01:44:10.23 SPRsummary:42.2RIU,57.7RIU,112RIU,154RIU after 1792s @01:55:40.81 delay 10 seconds @01:55:50.84 SPR.command "start 2 0" @01:55:53.52 Waiting up to 11:00 for SPR to enter state 1 @01:57:51.19 SPR.command "hold_on" @01:57:52.57 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:57:53.17 Lysis1.seek 300uL @01:57:53.29 Lysis2.seek 300uL @01:58:14.33 delay 15 seconds @01:58:29.35 SPR.command "hold_off" @01:58:32.42 Waiting up to 38:00 for SPR to enter state 7 @02:24:23.17 delay 10 seconds @02:24:33.19 SPR.command "start 2 0" @02:24:35.93 Waiting up to 11:00 for SPR to enter state 1 @02:26:33.55 SPR.command "hold_on" @02:26:34.99 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:26:35.59 Lysis1.seek 150uL @02:26:35.64 Lysis2.seek 150uL @02:26:56.73 delay 15 seconds @02:27:11.75 SPR.command "hold_off" @02:27:14.78 Waiting up to 38:00 for SPR to enter state 7 @02:53:05.55 delay 10 seconds @02:53:15.57 SPR.command "start 2 0" @02:53:18.77 Waiting up to 11:00 for SPR to enter state 1 @02:55:17.33 SPR.command "hold_on" @02:55:18.77 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:55:19.34 Lysis1.seek 0uL @02:55:19.38 Lysis2.seek 0uL @02:55:40.50 delay 15 seconds @02:55:55.53 SPR.command "hold_off" @02:55:58.09 Waiting up to 38:00 for SPR to enter state 7 @03:21:47.76 SPR analysis completed @03:21:51.78 Ended log spr23jul2201h.spr.bz2 @03:21:51.80 SPR.command "stats_off" @03:21:52.14 Handoff.select :close @03:21:52.29
Gate.power :heat,:OFF @03:21:52.31 Gate.power :cartridge,:OFF @03:21:52.39 PinPort.in :unlocking @03:21:52.90 PinPort.seek :clear @03:21:54.83 Carousel.in :default @03:21:54.96 Carousel.home.seek 45 @03:21:55.32 Plunge.seek :locked @03:21:55.49 Archive.in :unlocking @03:21:55.64 Archive.seek :clear @03:21:55.75 Air.in :unlocking @03:21:55.93 Air.seek :clear @03:21:56.02 Lysis1.in :unlocking @03:21:56.18 Lysis1.seek :clear @03:21:56.24 Lysis2.in :unlocking @03:21:56.36 Lysis2.seek :clear @03:22:00.96
Gate.power :motor,:OFF @03:22:00.98 Gate.power :core,:OFF @03:22:00.99 Gate.power :analytic1,:OFF @03:22:01.00 Gate.power :analytic2,:OFF @03:22:01.02 Gate.power :main,:OFF @03:22:01.03 Safely stopped and ready to power off @03:22:01.26 Gate.power :sampler,:OFF,limit to 3500mA @03:22:04.80 Gate.power :main,:ON,limit to 5000mA @03:22:04.81 Gate.power :analytic1,:ON,limit to 5000mA @03:22:04.83 Gate.power :analytic2,:ON,limit to 5000mA @03:22:05.06 Powering core bus @03:22:07.26 SPR Module 1, FW Rev. B, FPGA Rev. C @03:22:07.85
Archive.configure :default @03:22:07.94 Air.configure :default @03:22:08.01 Lysis1.configure :default @03:22:08.07 Lysis2.configure :default @03:22:08.17 Plunge.configure :default @03:22:08.26 SamplePump.configure :default @03:22:08.35 Toroid.configure :default @03:22:08.45 Carousel.configure :default @03:22:08.54 Pressure.configure :default @03:22:08.63 ExtraPump.configure :default @03:22:08.70 PinPort.configure :default @03:22:08.77 All elf controllers running firmware version 4.84 @03:22:08.79 Gate.power :motor,:ON,limit to 5000mA @03:22:09.14 Archive.home.jog 0 @03:22:09.85 PinPort.home.jog 0 @03:22:09.92 Carousel.home.jog 0 @03:22:09.95 Toroid.home.jog 0 @03:22:09.98 Lysis2.home.jog 0 @03:22:10.02 Lysis1.home.jog 0 @03:22:10.06 Air.home.jog 0 @03:22:10.09 Plunge.home.jog 0 @03:22:11.50 Archive.in :homing @03:22:11.85 Archive.jog 1373 @03:22:11.91 PinPort.in :homing @03:22:11.94 Plunge.in :homing @03:22:11.98 Lysis1.in :homing @03:22:12.02 Toroid.in :homing @03:22:12.08 Lysis2.in :homing @03:22:12.11 Air.in :homing @03:22:12.46 PinPort.jog -111 @03:22:12.49 Plunge.jog 1553 @03:22:12.55 Toroid.jog 597 @03:22:12.59 Lysis1.jog 1382 @03:22:12.65 Lysis2.jog 1394 @03:22:12.68 Air.jog 1365 @03:22:13.09 PinPort.setRawPosition! 0 @03:22:13.15 PinPort.seek :home @03:22:13.33 Archive.setRawPosition! 50 @03:22:13.35 PinPort.in :default @03:22:13.44 Archive.seek :home @03:22:13.75 Archive.in :default @03:22:13.97 Lysis1.setRawPosition! 50 @03:22:14.03 Lysis1.seek :home @03:22:14.15 Lysis2.setRawPosition! 50 @03:22:14.17 Air.setRawPosition! 50 @03:22:14.20 Plunge.setRawPosition! 50 @03:22:14.33 Lysis2.seek :home @03:22:14.37 Plunge.seek :home @03:22:14.41 Air.seek :home @03:22:14.62 Lysis1.in :default @03:22:14.72 Lysis2.in :default @03:22:14.86 Plunge.in :default @03:22:14.89 Air.in :default @03:22:15.04 Toroid.setRawPosition! 0 @03:22:15.10 Toroid.seek :home @03:22:15.19 Toroid.in :default @03:22:15.33
Toroid.seek :clear @03:22:16.94 Carousel.in :default @03:22:17.07 Carousel.home.seek 45 @03:22:17.35 Selecting Cartridge 44 @03:22:17.66 Carousel.seek 44 @03:22:18.20 Lysis1.in :fast @03:22:18.26 Lysis1.seek :locked @03:22:20.88 Plunge.seek :locked @03:22:21.05 Air.in :fast @03:22:21.11 Air.seek :locked @03:22:22.69
Carousel.reconfigure :locked @03:22:23.38 Powering cartridge bus @03:22:26.04 Gate.setIR 1750,5000,0,6 @03:22:26.16 Gate.setIR 0,5000,0,6 @03:22:26.23 PuckHeater.configure :default @03:22:26.29 Gate.power :heat,:ON,limit to 5000mA @03:22:26.93 Mounted lysis Cartridge @03:22:26.95 WARNING: Using default puck temperature sensor calibration @03:22:26.97 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:22:28.56 SPR.command "read_sequences" @03:22:28.89 SPR.command "stats_on" @03:22:29.06 SPR.command "start 0 1" @03:22:29.76 SPR.start /var/log/methane/spr23jul2203h.spr.bz2 @03:22:31.75 Waiting for baseline SPR stabilization @03:22:31.76 Handoff.select :waste @03:22:38.48 Syringe tare is 20% of full scale @03:22:38.50 PinPort.in :locking @03:22:38.57 PinPort.seek :locked @03:22:41.11 Archive.in :fast @03:22:41.18 Lysis2.in :fast @03:22:41.27 Lysis1.seek 1000uL @03:22:41.51 Lysis2.seek 1000uL @03:22:41.55 Archive.seek :full @03:22:46.05 delayUntil "03:32:31.31" @03:32:31.33 Lysis1.in :DAmix @03:32:31.46 Lysis2.in :DAmix @03:32:32.03 Lysis1.seek 750uL @03:32:32.14 Lysis2.seek 750uL @03:33:07.26 delay 5 seconds @03:33:12.28 Handoff.select SPR @03:33:12.41 SPR.command "start 2 14" @03:33:13.01 SPR stabilized @03:33:13.19 delay 10 seconds @03:33:23.24 SPR.command "start 2 0" @03:33:26.38 Waiting up to 11:00 for SPR to enter state 1 @03:35:24.06 SPR.command "hold_on" @03:35:25.97 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @03:35:26.58 Lysis1.seek 450uL @03:35:26.63 Lysis2.seek 450uL @03:36:08.70 delay 15 seconds @03:36:23.72 SPR.command "hold_off" @03:36:27.04 Waiting up to 38:00 for SPR to enter state 7 @03:50:46.28 SPRsummary:41.2RIU,38.9RIU,92.1RIU,111RIU after 1791s @04:02:16.87 delay 10 seconds @04:02:26.89 SPR.command "start 2 0" @04:02:30.08 Waiting up to 11:00 for SPR to enter state 1 @04:04:28.18 SPR.command "hold_on" @04:04:29.56 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @04:04:30.17 Lysis1.seek 300uL @04:04:30.21 Lysis2.seek 300uL @04:04:51.33 delay 15 seconds @04:05:06.35 SPR.command "hold_off" @04:05:09.40 Waiting up to 38:00 for SPR to enter state 7 @04:31:00.17 delay 10 seconds @04:31:10.19 SPR.command "start 2 0" @04:31:13.39 Waiting up to 11:00 for SPR to enter state 1 @04:33:11.48 SPR.command "hold_on" @04:33:12.87 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @04:33:13.96 Lysis1.seek 150uL @04:33:14.02 Lysis2.seek 150uL @04:33:35.13 delay 15 seconds @04:33:50.16 SPR.command "hold_off" @04:33:53.26 Waiting up to 38:00 for SPR to enter state 7 @04:59:44.42 delay 10 seconds @04:59:54.44 SPR.command "start 2 0" @04:59:57.12 Waiting up to 11:00 for SPR to enter state 1 @05:01:54.80 SPR.command "hold_on" @05:01:56.18 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:01:57.30 Lysis1.seek 0uL @05:01:57.34 Lysis2.seek 0uL @05:02:18.45 delay 15 seconds @05:02:33.48 SPR.command "hold_off" @05:02:36.02 Waiting up to 38:00 for SPR to enter state 7 @05:28:26.63 SPR analysis completed @05:28:30.73 Ended log spr23jul2203h.spr.bz2 @05:28:30.75 SPR.command "stats_off" @05:28:31.54 Handoff.select :close @05:28:31.69
Gate.power :heat,:OFF @05:28:31.71 Gate.power :cartridge,:OFF @05:28:31.79 PinPort.in :unlocking @05:28:31.86 PinPort.seek :clear @05:28:34.22 Carousel.in :default @05:28:34.36 Carousel.home.seek 44 @05:28:34.70 Plunge.seek :locked @05:28:34.88 Archive.in :unlocking @05:28:35.04 Archive.seek :clear @05:28:35.12 Air.in :unlocking @05:28:35.36 Air.seek :clear @05:28:35.47 Lysis1.in :unlocking @05:28:35.65 Lysis1.seek :clear @05:28:35.73 Lysis2.in :unlocking @05:28:35.83 Lysis2.seek :clear @05:28:40.45
Gate.power :motor,:OFF @05:28:40.47 Gate.power :core,:OFF @05:28:40.48 Gate.power :analytic1,:OFF @05:28:40.50 Gate.power :analytic2,:OFF @05:28:40.51 Gate.power :main,:OFF @05:28:40.52 Safely stopped and ready to power off @05:28:40.71 Gate.power :sampler,:OFF,limit to 3500mA @05:28:44.29 Gate.power :main,:ON,limit to 5000mA @05:28:44.31 Gate.power :analytic1,:ON,limit to 5000mA @05:28:44.32 Gate.power :analytic2,:ON,limit to 5000mA @05:28:44.44 Powering core bus @05:28:46.75 SPR Module 1, FW Rev. B, FPGA Rev. C @05:28:47.24
Archive.configure :default @05:28:47.35 Air.configure :default @05:28:47.42 Lysis1.configure :default @05:28:47.49 Lysis2.configure :default @05:28:47.58 Plunge.configure :default @05:28:47.67 SamplePump.configure :default @05:28:47.77 Toroid.configure :default @05:28:47.86 Carousel.configure :default @05:28:47.96 Pressure.configure :default @05:28:48.05 ExtraPump.configure :default @05:28:48.11 PinPort.configure :default @05:28:48.18 All elf controllers running firmware version 4.84 @05:28:48.20 Gate.power :motor,:ON,limit to 5000mA @05:28:48.41 Archive.home.jog 0 @05:28:49.19 PinPort.home.jog 0 @05:28:49.24 Toroid.home.jog 0 @05:28:49.30 Plunge.home.jog 0 @05:28:49.33 Lysis1.home.jog 0 @05:28:49.36 Air.home.jog 0 @05:28:49.40 Carousel.home.jog 0 @05:28:49.44 Lysis2.home.jog 0 @05:28:50.88 Archive.in :homing @05:28:51.13 Archive.jog 1370 @05:28:51.20 PinPort.in :homing @05:28:51.26 Plunge.in :homing @05:28:51.32 Lysis1.in :homing @05:28:51.36 Toroid.in :homing @05:28:51.41 Lysis2.in :homing @05:28:51.45 Air.in :homing @05:28:51.81 PinPort.jog -111 @05:28:51.87 Toroid.jog 599 @05:28:51.94 Plunge.jog 1551 @05:28:51.98 Lysis1.jog 1383 @05:28:52.01 Air.jog 1366 @05:28:52.04 Lysis2.jog 1393 @05:28:52.48 PinPort.setRawPosition! 0 @05:28:52.55 PinPort.seek :home @05:28:52.66 PinPort.in :default @05:28:52.74 Archive.setRawPosition! 50 @05:28:52.80 Archive.seek :home @05:28:53.07 Archive.in :default @05:28:53.40 Air.setRawPosition! 50 @05:28:53.48 Air.seek :home @05:28:53.57 Plunge.setRawPosition! 50 @05:28:53.61 Lysis2.setRawPosition! 50 @05:28:53.64 Lysis1.setRawPosition! 50 @05:28:53.74 Plunge.seek :home @05:28:53.81 Lysis1.seek :home @05:28:53.89 Lysis2.seek :home @05:28:54.07 Air.in :default @05:28:54.18 Plunge.in :default @05:28:54.29 Toroid.setRawPosition! 0 @05:28:54.33 Lysis1.in :default @05:28:54.36 Lysis2.in :default @05:28:54.53 Toroid.seek :home @05:28:54.63 Toroid.in :default @05:28:54.81
Toroid.seek :clear @05:28:56.42 Carousel.in :default @05:28:56.55 Carousel.home.seek 44 @05:28:56.82 Selecting Cartridge 43 @05:28:57.14 Carousel.seek 43 @05:28:57.68 Lysis1.in :fast @05:28:57.74 Lysis1.seek :locked @05:29:00.37 Plunge.seek :locked @05:29:00.54 Air.in :fast @05:29:00.60 Air.seek :locked @05:29:02.18
Carousel.reconfigure :locked @05:29:02.35 Powering cartridge bus @05:29:05.23 Gate.setIR 1750,5000,0,6 @05:29:05.36 Gate.setIR 0,5000,0,6 @05:29:05.43 PuckHeater.configure :default @05:29:05.50 Gate.power :heat,:ON,limit to 5000mA @05:29:06.13 Mounted lysis Cartridge @05:29:06.16 WARNING: Using default puck temperature sensor calibration @05:29:06.18 Using default cartridge heat loss curve for HeatingAccelInCarousel @05:29:07.73 SPR.command "read_sequences" @05:29:08.39 SPR.command "stats_on" @05:29:08.55 SPR.command "start 0 1" @05:29:09.25 SPR.start /var/log/methane/spr23jul2205h.spr.bz2 @05:29:11.26 Waiting for baseline SPR stabilization @05:29:11.28 Handoff.select :waste @05:29:17.99 Syringe tare is 20% of full scale @05:29:18.01 PinPort.in :locking @05:29:18.08 PinPort.seek :locked @05:29:20.61 Archive.in :fast @05:29:20.66 Lysis2.in :fast @05:29:20.74 Lysis1.seek 1000uL @05:29:20.92 Archive.seek :full @05:29:20.99 Lysis2.seek 1000uL @05:29:25.52 delayUntil "05:39:10.81" @05:39:10.82 Lysis1.in :DAmix @05:39:10.95 Lysis2.in :DAmix @05:39:11.58 Lysis1.seek 750uL @05:39:11.64 Lysis2.seek 750uL @05:39:46.76 delay 5 seconds @05:39:51.78 Handoff.select SPR @05:39:51.91 SPR.command "start 2 14" @05:39:53.03 SPR stabilized @05:39:53.20 delay 10 seconds @05:40:03.24 SPR.command "start 2 0" @05:40:05.88 Waiting up to 11:00 for SPR to enter state 1 @05:42:03.55 SPR.command "hold_on" @05:42:05.47 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @05:42:06.08 Lysis1.seek 450uL @05:42:06.14 Lysis2.seek 450uL @05:42:48.20 delay 15 seconds @05:43:03.23 SPR.command "hold_off" @05:43:06.54 Waiting up to 38:00 for SPR to enter state 7 @05:57:25.77 SPRsummary:37.2RIU,36.3RIU,90.8RIU,103RIU after 1791s @06:08:56.36 delay 10 seconds @06:09:06.38 SPR.command "start 2 0" @06:09:09.58 Waiting up to 11:00 for SPR to enter state 1 @06:11:07.67 SPR.command "hold_on" @06:11:09.06 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @06:11:09.67 Lysis1.seek 300uL @06:11:09.75 Lysis2.seek 300uL @06:11:30.86 delay 15 seconds @06:11:45.88 SPR.command "hold_off" @06:11:48.89 Waiting up to 38:00 for SPR to enter state 7 @06:37:39.66 delay 10 seconds @06:37:49.68 SPR.command "start 2 0" @06:37:52.37 Waiting up to 11:00 for SPR to enter state 1 @06:39:50.50 SPR.command "hold_on" @06:39:51.42 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @06:39:52.02 Lysis1.seek 150uL @06:39:52.06 Lysis2.seek 150uL @06:40:13.16 delay 15 seconds @06:40:28.18 SPR.command "hold_off" @06:40:31.28 Waiting up to 38:00 for SPR to enter state 7 @07:06:22.02 delay 10 seconds @07:06:32.05 SPR.command "start 2 0" @07:06:35.29 Waiting up to 11:00 for SPR to enter state 1 @07:08:33.34 SPR.command "hold_on" @07:08:34.73 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @07:08:35.36 Lysis1.seek 0uL @07:08:35.41 Lysis2.seek 0uL @07:08:56.53 delay 15 seconds @07:09:11.55 SPR.command "hold_off" @07:09:14.57 Waiting up to 38:00 for SPR to enter state 7 @07:35:05.18 SPR analysis completed @07:35:09.26 Ended log spr23jul2205h.spr.bz2 @07:35:09.28 SPR.command "stats_off" @07:35:10.02 Handoff.select :close @07:35:10.17
Gate.power :heat,:OFF @07:35:10.20 Gate.power :cartridge,:OFF @07:35:10.28 PinPort.in :unlocking @07:35:10.35 PinPort.seek :clear @07:35:12.26 Carousel.in :default @07:35:12.39 Carousel.home.seek 43 @07:35:12.72 Plunge.seek :locked @07:35:12.90 Archive.in :unlocking @07:35:13.08 Archive.seek :clear @07:35:13.13 Air.in :unlocking @07:35:13.34 Air.seek :clear @07:35:13.46 Lysis1.in :unlocking @07:35:13.64 Lysis1.seek :clear @07:35:13.70 Lysis2.in :unlocking @07:35:13.82 Lysis2.seek :clear @07:35:18.42
Gate.power :motor,:OFF @07:35:18.44 Gate.power :core,:OFF @07:35:18.45 Gate.power :analytic1,:OFF @07:35:18.46 Gate.power :analytic2,:OFF @07:35:18.48 Gate.power :main,:OFF @07:35:18.49 Safely stopped and ready to power off @07:35:18.68 Gate.power :sampler,:OFF,limit to 3500mA @07:35:22.26 Gate.power :main,:ON,limit to 5000mA @07:35:22.28 Gate.power :analytic1,:ON,limit to 5000mA @07:35:22.29 Gate.power :analytic2,:ON,limit to 5000mA @07:35:22.42 Powering core bus @07:35:24.72 SPR Module 1, FW Rev. B, FPGA Rev. C @07:35:25.26
Archive.configure :default @07:35:25.36 Air.configure :default @07:35:25.42 Lysis1.configure :default @07:35:25.49 Lysis2.configure :default @07:35:25.58 Plunge.configure :default @07:35:25.68 SamplePump.configure :default @07:35:25.77 Toroid.configure :default @07:35:25.87 Carousel.configure :default @07:35:25.96 Pressure.configure :default @07:35:26.05 ExtraPump.configure :default @07:35:26.11 PinPort.configure :default @07:35:26.18 All elf controllers running firmware version 4.84 @07:35:26.20 Gate.power :motor,:ON,limit to 5000mA @07:35:26.38 Archive.home.jog 0 @07:35:27.19 PinPort.home.jog 0 @07:35:27.29 Carousel.home.jog 0 @07:35:27.34 Toroid.home.jog 0 @07:35:27.37 Lysis2.home.jog 0 @07:35:27.39 Lysis1.home.jog 0 @07:35:27.42 Air.home.jog 0 @07:35:27.46 Plunge.home.jog 0 @07:35:28.83 Archive.in :homing @07:35:29.20 Archive.jog 1370 @07:35:29.31 PinPort.in :homing @07:35:29.35 Plunge.in :homing @07:35:29.38 Air.in :homing @07:35:29.42 Lysis2.in :homing @07:35:29.46 Toroid.in :homing @07:35:29.51 Lysis1.in :homing @07:35:29.87 PinPort.jog -111 @07:35:29.90 Lysis2.jog 1395 @07:35:29.97 Air.jog 1365 @07:35:30.01 Plunge.jog 1550 @07:35:30.07 Lysis1.jog 1380 @07:35:30.12 Toroid.jog 595 @07:35:30.51 PinPort.setRawPosition! 0 @07:35:30.57 PinPort.seek :home @07:35:30.67 PinPort.in :default @07:35:30.76 Archive.setRawPosition! 50 @07:35:30.82 Archive.seek :home @07:35:31.10 Archive.in :default @07:35:31.34 Lysis2.setRawPosition! 50 @07:35:31.40 Lysis2.seek :home @07:35:31.50 Air.setRawPosition! 50 @07:35:31.56 Air.seek :home @07:35:31.65 Lysis1.setRawPosition! 50 @07:35:31.69 Plunge.setRawPosition! 50 @07:35:31.78 Lysis1.seek :home @07:35:31.86 Plunge.seek :home @07:35:32.02 Air.in :default @07:35:32.04 Lysis2.in :default @07:35:32.23 Lysis1.in :default @07:35:32.25 Plunge.in :default @07:35:32.42 Toroid.setRawPosition! 0 @07:35:32.47 Toroid.seek :home @07:35:32.58 Toroid.in :default @07:35:32.69
Toroid.seek :clear @07:35:34.30 Carousel.in :default @07:35:34.43 Carousel.home.seek 43 @07:35:34.69 Selecting Cartridge 42 @07:35:35.01 Carousel.seek 42 @07:35:35.55 Lysis1.in :fast @07:35:35.61 Lysis1.seek :locked @07:35:38.23 Plunge.seek :locked @07:35:38.90 Air.in :fast @07:35:38.97 Air.seek :locked @07:35:40.55
Carousel.reconfigure :locked @07:35:40.72 Powering cartridge bus @07:35:43.38 Gate.setIR 1750,5000,0,6 @07:35:43.50 Gate.setIR 0,5000,0,6 @07:35:43.57 PuckHeater.configure :default @07:35:43.63 Gate.power :heat,:ON,limit to 5000mA @07:35:44.27 Mounted lysis Cartridge @07:35:44.30 WARNING: Using default puck temperature sensor calibration @07:35:44.31 Using default cartridge heat loss curve for HeatingAccelInCarousel @07:35:45.86 SPR.command "read_sequences" @07:35:46.35 SPR.command "stats_on" @07:35:46.52 SPR.command "start 0 1" @07:35:47.23 SPR.start /var/log/methane/spr23jul2207h.spr.bz2 @07:35:49.21 Waiting for baseline SPR stabilization @07:35:49.25 Handoff.select :waste @07:35:55.97 Syringe tare is 20% of full scale @07:35:55.99 PinPort.in :locking @07:35:56.06 PinPort.seek :locked @07:35:58.60 Archive.in :fast @07:35:58.68 Lysis2.in :fast @07:35:58.76 Archive.seek :full @07:35:58.81 Lysis1.seek 1000uL @07:35:58.96 Lysis2.seek 1000uL @07:36:03.47 delayUntil "07:45:48.78" @07:45:48.79 Lysis1.in :DAmix @07:45:48.92 Lysis2.in :DAmix @07:45:49.53 Lysis1.seek 750uL @07:45:49.59 Lysis2.seek 750uL @07:46:24.72 delay 5 seconds @07:46:29.74 Handoff.select SPR @07:46:29.87 SPR.command "start 2 14" @07:46:30.48 SPR stabilized @07:46:30.64 delay 10 seconds @07:46:40.66 SPR.command "start 2 0" @07:46:43.84 Waiting up to 11:00 for SPR to enter state 1 @07:48:41.52 SPR.command "hold_on" @07:48:43.43 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:48:44.04 Lysis1.seek 450uL @07:48:44.07 Lysis2.seek 450uL @07:49:26.14 delay 15 seconds @07:49:41.16 SPR.command "hold_off" @07:49:44.50 Waiting up to 38:00 for SPR to enter state 7 @08:04:03.74 SPRsummary:36.1RIU,32.4RIU,92.3RIU,99.8RIU after 1791s @08:15:34.32 delay 10 seconds @08:15:44.34 SPR.command "start 2 0" @08:15:47.01 Waiting up to 11:00 for SPR to enter state 1 @08:17:44.69 SPR.command "hold_on" @08:17:46.07 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:17:46.68 Lysis1.seek 300uL @08:17:46.72 Lysis2.seek 300uL @08:18:07.82 delay 15 seconds @08:18:22.84 SPR.command "hold_off" @08:18:25.91 Waiting up to 38:00 for SPR to enter state 7 @08:44:17.16 delay 10 seconds @08:44:27.18 SPR.command "start 2 0" @08:44:30.32 Waiting up to 11:00 for SPR to enter state 1 @08:46:28.00 SPR.command "hold_on" @08:46:29.38 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:46:29.97 Lysis1.seek 150uL @08:46:30.01 Lysis2.seek 150uL @08:46:51.13 delay 15 seconds @08:47:06.15 SPR.command "hold_off" @08:47:09.24 Waiting up to 38:00 for SPR to enter state 7 @09:12:59.99 delay 10 seconds @09:13:10.01 SPR.command "start 2 0" @09:13:12.70 Waiting up to 11:00 for SPR to enter state 1 @09:15:10.37 SPR.command "hold_on" @09:15:11.74 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @09:15:12.35 Lysis1.seek 0uL @09:15:12.42 Lysis2.seek 0uL @09:15:33.51 delay 15 seconds @09:15:48.53 SPR.command "hold_off" @09:15:51.60 Waiting up to 38:00 for SPR to enter state 7 @09:41:42.21 SPR analysis completed @09:41:46.34 Ended log spr23jul2207h.spr.bz2 @09:41:46.36 SPR.command "stats_off" @09:41:47.56 Handoff.select :close @09:41:47.72
Gate.power :heat,:OFF @09:41:47.73 Gate.power :cartridge,:OFF @09:41:47.82 PinPort.in :unlocking @09:41:47.88 PinPort.seek :clear @09:41:49.79 Carousel.in :default @09:41:49.92 Carousel.home.seek 42 @09:41:50.26 Plunge.seek :locked @09:41:50.43 Archive.in :unlocking @09:41:50.59 Archive.seek :clear @09:41:50.65 Air.in :unlocking @09:41:50.85 Air.seek :clear @09:41:50.96 Lysis1.in :unlocking @09:41:51.15 Lysis1.seek :clear @09:41:51.20 Lysis2.in :unlocking @09:41:51.37 Lysis2.seek :clear @09:41:55.97
Gate.power :motor,:OFF @09:41:55.99 Gate.power :core,:OFF @09:41:56.00 Gate.power :analytic1,:OFF @09:41:56.01 Gate.power :analytic2,:OFF @09:41:56.03 Gate.power :main,:OFF @09:41:56.04 Safely stopped and ready to power off @09:41:56.51 Gate.power :sampler,:OFF,limit to 3500mA @09:41:59.81 Gate.power :main,:ON,limit to 5000mA @09:41:59.82 Gate.power :analytic1,:ON,limit to 5000mA @09:41:59.84 Gate.power :analytic2,:ON,limit to 5000mA @09:42:00.16 Powering core bus @09:42:02.28 SPR Module 1, FW Rev. B, FPGA Rev. C @09:42:02.94
Archive.configure :default @09:42:03.03 Air.configure :default @09:42:03.10 Lysis1.configure :default @09:42:03.16 Lysis2.configure :default @09:42:03.28 Plunge.configure :default @09:42:03.37 SamplePump.configure :default @09:42:03.47 Toroid.configure :default @09:42:03.57 Carousel.configure :default @09:42:03.66 Pressure.configure :default @09:42:03.75 ExtraPump.configure :default @09:42:03.82 PinPort.configure :default @09:42:03.88 All elf controllers running firmware version 4.84 @09:42:03.90 Gate.power :motor,:ON,limit to 5000mA @09:42:04.21 Archive.home.jog 0 @09:42:04.88 PinPort.home.jog 0 @09:42:04.91 Toroid.home.jog 0 @09:42:04.97 Plunge.home.jog 0 @09:42:05.01 Lysis1.home.jog 0 @09:42:05.61 Air.home.jog 0 @09:42:05.68 Carousel.home.jog 0 @09:42:05.71 Lysis2.home.jog 0 @09:42:06.48 Archive.in :homing @09:42:06.71 Archive.jog 1370 @09:42:06.93 Toroid.in :homing @09:42:06.98 Plunge.in :homing @09:42:07.01 Lysis1.in :homing @09:42:07.07 PinPort.in :homing @09:42:07.10 Lysis2.in :homing @09:42:07.15 Air.in :homing @09:42:07.43 Toroid.jog 588 @09:42:07.55 Plunge.jog 1551 @09:42:07.58 Lysis1.jog 1380 @09:42:07.62 Lysis2.jog 1396 @09:42:07.66 PinPort.jog -111 @09:42:07.76 Air.jog 1368 @09:42:08.14 Archive.setRawPosition! 50 @09:42:08.22 PinPort.setRawPosition! 0 @09:42:08.25 Archive.seek :home @09:42:08.36 PinPort.seek :home @09:42:08.46 PinPort.in :default @09:42:08.58 Archive.in :default @09:42:09.08 Lysis2.setRawPosition! 50 @09:42:09.11 Lysis1.setRawPosition! 50 @09:42:09.13 Plunge.setRawPosition! 50 @09:42:09.30 Lysis2.seek :home @09:42:09.36 Lysis1.seek :home @09:42:09.40 Plunge.seek :home @09:42:09.65 Air.setRawPosition! 50 @09:42:09.71 Air.seek :home @09:42:09.84 Plunge.in :default @09:42:09.88 Lysis2.in :default @09:42:09.91 Toroid.setRawPosition! 0 @09:42:09.96 Lysis1.in :default @09:42:10.15 Toroid.seek :home @09:42:10.31 Air.in :default @09:42:10.34 Toroid.in :default @09:42:10.72
Toroid.seek :clear @09:42:12.34 Carousel.in :default @09:42:12.47 Carousel.home.seek 42 @09:42:12.73 Selecting Cartridge 41 @09:42:13.04 Carousel.seek 41 @09:42:13.59 Lysis1.in :fast @09:42:13.66 Lysis1.seek :locked @09:42:16.77 Plunge.seek :locked @09:42:16.94 Air.in :fast @09:42:17.01 Air.seek :locked @09:42:18.59
Carousel.reconfigure :locked @09:42:18.76 Powering cartridge bus @09:42:21.41 Gate.setIR 1750,5000,0,6 @09:42:21.54 Gate.setIR 0,5000,0,6 @09:42:21.61 PuckHeater.configure :default @09:42:21.67 Gate.power :heat,:ON,limit to 5000mA @09:42:22.30 Mounted lysis Cartridge @09:42:22.33 WARNING: Using default puck temperature sensor calibration @09:42:22.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:42:23.90 SPR.command "read_sequences" @09:42:23.96 SPR.command "stats_on" @09:42:24.12 SPR.command "start 0 1" @09:42:24.82 SPR.start /var/log/methane/spr23jul2209h.spr.bz2 @09:42:26.76 Waiting for baseline SPR stabilization @09:42:26.77 Handoff.select :waste @09:42:33.48 Syringe tare is 20% of full scale @09:42:33.50 PinPort.in :locking @09:42:33.57 PinPort.seek :locked @09:42:36.11 Archive.in :fast @09:42:36.17 Lysis2.in :fast @09:42:36.24 Lysis1.seek 1000uL @09:42:36.38 Archive.seek :full @09:42:36.50 Lysis2.seek 1000uL @09:42:41.05 delayUntil "09:52:26.33" @09:52:26.34 Lysis1.in :DAmix @09:52:26.47 Lysis2.in :DAmix @09:52:27.07 Lysis1.seek 750uL @09:52:27.12 Lysis2.seek 750uL @09:53:02.30 delay 5 seconds @09:53:07.32 Handoff.select SPR @09:53:07.45 SPR.command "start 2 14" @09:53:08.03 SPR stabilized @09:53:08.19 delay 10 seconds @09:53:18.21 SPR.command "start 2 0" @09:53:21.92 Waiting up to 11:00 for SPR to enter state 1 @09:55:20.01 SPR.command "hold_on" @09:55:21.92 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:55:22.53 Lysis1.seek 450uL @09:55:22.63 Lysis2.seek 450uL @09:56:04.63 delay 15 seconds @09:56:19.65 SPR.command "hold_off" @09:56:23.00 Waiting up to 38:00 for SPR to enter state 7 @10:10:42.23 SPRsummary:44.8RIU,37.1RIU,88.8RIU,101RIU after 1792s @10:22:12.82 delay 10 seconds @10:22:22.84 SPR.command "start 2 0" @10:22:25.51 Waiting up to 11:00 for SPR to enter state 1 @10:24:23.17 SPR.command "hold_on" @10:24:24.56 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:24:25.20 Lysis1.seek 300uL @10:24:25.29 Lysis2.seek 300uL @10:24:46.39 delay 15 seconds @10:25:01.41 SPR.command "hold_off" @10:25:04.41 Waiting up to 38:00 for SPR to enter state 7 @10:50:55.17 delay 10 seconds @10:51:05.20 SPR.command "start 2 0" @10:51:08.40 Waiting up to 11:00 for SPR to enter state 1 @10:53:06.49 SPR.command "hold_on" @10:53:08.40 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:53:08.95 Lysis1.seek 150uL @10:53:08.99 Lysis2.seek 150uL @10:53:30.09 delay 15 seconds @10:53:45.12 SPR.command "hold_off" @10:53:47.72 Waiting up to 38:00 for SPR to enter state 7 @11:19:37.53 delay 10 seconds @11:19:47.56 SPR.command "start 2 0" @11:19:50.23 Waiting up to 11:00 for SPR to enter state 1 @11:21:47.91 SPR.command "hold_on" @11:21:49.29 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @11:21:50.38 Lysis1.seek 0uL @11:21:50.44 Lysis2.seek 0uL @11:22:11.53 delay 15 seconds @11:22:26.57 SPR.command "hold_off" @11:22:29.13 Waiting up to 38:00 for SPR to enter state 7 @11:48:19.75 SPR analysis completed @11:48:23.85 Ended log spr23jul2209h.spr.bz2 @11:48:23.87 SPR.command "stats_off" @11:48:24.62 Handoff.select :close @11:48:24.77
Gate.power :heat,:OFF @11:48:24.78 Gate.power :cartridge,:OFF @11:48:24.87 PinPort.in :unlocking @11:48:24.93 PinPort.seek :clear @11:48:26.84 Carousel.in :default @11:48:26.97 Carousel.home.seek 41 @11:48:27.34 Plunge.seek :locked @11:48:27.51 Archive.in :unlocking @11:48:27.70 Archive.seek :clear @11:48:27.76 Air.in :unlocking @11:48:27.93 Air.seek :clear @11:48:28.07 Lysis1.in :unlocking @11:48:28.21 Lysis1.seek :clear @11:48:28.32 Lysis2.in :unlocking @11:48:28.41 Lysis2.seek :clear @11:48:33.01
Gate.power :motor,:OFF @11:48:33.03 Gate.power :core,:OFF @11:48:33.04 Gate.power :analytic1,:OFF @11:48:33.06 Gate.power :analytic2,:OFF @11:48:33.07 Gate.power :main,:OFF @11:48:33.08 Safely stopped and ready to power off @11:48:33.28 Gate.power :sampler,:OFF,limit to 3500mA @11:48:36.85 Gate.power :main,:ON,limit to 5000mA @11:48:36.87 Gate.power :analytic1,:ON,limit to 5000mA @11:48:36.88 Gate.power :analytic2,:ON,limit to 5000mA @11:48:37.00 Powering core bus @11:48:39.34 SPR Module 1, FW Rev. B, FPGA Rev. C @11:48:39.78
Archive.configure :default @11:48:39.87 Air.configure :default @11:48:39.94 Lysis1.configure :default @11:48:40.00 Lysis2.configure :default @11:48:40.10 Plunge.configure :default @11:48:40.19 SamplePump.configure :default @11:48:40.29 Toroid.configure :default @11:48:40.38 Carousel.configure :default @11:48:40.47 Pressure.configure :default @11:48:40.57 ExtraPump.configure :default @11:48:40.64 PinPort.configure :default @11:48:40.71 All elf controllers running firmware version 4.84 @11:48:40.73 Gate.power :motor,:ON,limit to 5000mA @11:48:40.90 Archive.home.jog 0 @11:48:41.79 PinPort.home.jog 0 @11:48:41.84 Carousel.home.jog 0 @11:48:41.89 Plunge.home.jog 0 @11:48:41.92 Lysis2.home.jog 0 @11:48:41.95 Air.home.jog 0 @11:48:41.98 Toroid.home.jog 0 @11:48:42.02 Lysis1.home.jog 0 @11:48:43.53 Archive.in :homing @11:48:43.71 Archive.jog 1371 @11:48:43.93 Toroid.in :homing @11:48:43.98 Plunge.in :homing @11:48:44.02 Lysis1.in :homing @11:48:44.06 PinPort.in :homing @11:48:44.11 Air.in :homing @11:48:44.17 Lysis2.in :homing @11:48:44.39 Toroid.jog 591 @11:48:44.45 Plunge.jog 1550 @11:48:44.56 PinPort.jog -111 @11:48:44.61 Lysis1.jog 1379 @11:48:44.69 Lysis2.jog 1394 @11:48:44.74 Air.jog 1365 @11:48:45.12 Archive.setRawPosition! 50 @11:48:45.14 PinPort.setRawPosition! 0 @11:48:45.28 Archive.seek :home @11:48:45.31 PinPort.seek :home @11:48:45.50 PinPort.in :default @11:48:45.63 Archive.in :default @11:48:46.11 Lysis1.setRawPosition! 50 @11:48:46.16 Lysis1.seek :home @11:48:46.27 Plunge.setRawPosition! 50 @11:48:46.29 Lysis2.setRawPosition! 50 @11:48:46.32 Air.setRawPosition! 50 @11:48:46.47 Lysis2.seek :home @11:48:46.50 Air.seek :home @11:48:46.53 Plunge.seek :home @11:48:46.79 Toroid.setRawPosition! 0 @11:48:46.81 Lysis1.in :default @11:48:46.92 Toroid.seek :home @11:48:47.09 Plunge.in :default @11:48:47.13 Lysis2.in :default @11:48:47.16 Toroid.in :default @11:48:47.20 Air.in :default @11:48:47.72
Toroid.seek :clear @11:48:49.33 Carousel.in :default @11:48:49.48 Carousel.home.seek 41 @11:48:49.76 Selecting Cartridge 40 @11:48:50.07 Carousel.seek 40 @11:48:50.60 Lysis1.in :fast @11:48:50.67 Lysis1.seek :locked @11:48:53.33 Plunge.seek :locked @11:48:53.50 Air.in :fast @11:48:54.07 Air.seek :locked @11:48:55.65
Carousel.reconfigure :locked @11:48:55.83 Powering cartridge bus @11:48:58.49 Gate.setIR 1750,5000,0,6 @11:48:58.61 Gate.setIR 0,5000,0,6 @11:48:58.69 PuckHeater.configure :default @11:48:58.75 Gate.power :heat,:ON,limit to 5000mA @11:48:59.38 Mounted lysis Cartridge @11:48:59.41 WARNING: Using default puck temperature sensor calibration @11:48:59.43 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:49:01.00 SPR.command "read_sequences" @11:49:01.06 SPR.command "stats_on" @11:49:01.23 SPR.command "start 0 1" @11:49:01.92 SPR.start /var/log/methane/spr23jul2211h.spr.bz2 @11:49:03.80 Waiting for baseline SPR stabilization @11:49:03.82 Handoff.select :waste @11:49:10.52 Syringe tare is 20% of full scale @11:49:10.55 PinPort.in :locking @11:49:10.61 PinPort.seek :locked @11:49:13.15 Archive.in :fast @11:49:13.23 Lysis2.in :fast @11:49:13.37 Lysis1.seek 1000uL @11:49:13.45 Archive.seek :full @11:49:13.56 Lysis2.seek 1000uL @11:49:18.12 delayUntil "11:59:03.37" @11:59:03.38 Lysis1.in :DAmix @11:59:03.51 Lysis2.in :DAmix @11:59:04.12 Lysis1.seek 750uL @11:59:04.17 Lysis2.seek 750uL @11:59:39.30 delay 5 seconds @11:59:44.32 Handoff.select SPR @11:59:44.45 SPR.command "start 2 14" @11:59:45.08 SPR stabilized @11:59:45.27 delay 10 seconds @11:59:55.30 SPR.command "start 2 0" @11:59:58.44 Waiting up to 11:00 for SPR to enter state 1 @12:01:56.11 SPR.command "hold_on" @12:01:58.02 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:01:58.63 Lysis1.seek 450uL @12:01:58.68 Lysis2.seek 450uL @12:02:40.72 delay 15 seconds @12:02:55.74 SPR.command "hold_off" @12:02:59.10 Waiting up to 38:00 for SPR to enter state 7 @12:17:18.33 SPRsummary:29.1RIU,31RIU,77.5RIU,99.7RIU after 1791s @12:28:48.91 delay 10 seconds @12:28:58.93 SPR.command "start 2 0" @12:29:01.61 Waiting up to 11:00 for SPR to enter state 1 @12:30:59.29 SPR.command "hold_on" @12:31:01.20 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:31:01.82 Lysis1.seek 300uL @12:31:01.87 Lysis2.seek 300uL @12:31:22.97 delay 15 seconds @12:31:37.99 SPR.command "hold_off" @12:31:40.52 Waiting up to 38:00 for SPR to enter state 7 @12:57:30.81 delay 10 seconds @12:57:40.83 SPR.command "start 2 0" @12:57:43.98 Waiting up to 11:00 for SPR to enter state 1 @12:59:41.65 SPR.command "hold_on" @12:59:43.03 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:59:43.66 Lysis1.seek 150uL @12:59:43.72 Lysis2.seek 150uL @13:00:04.84 delay 15 seconds @13:00:19.86 SPR.command "hold_off" @13:00:23.40 Waiting up to 38:00 for SPR to enter state 7 @13:26:15.08 delay 10 seconds @13:26:25.10 SPR.command "start 2 0" @13:26:28.24 Waiting up to 11:00 for SPR to enter state 1 @13:28:25.89 SPR.command "hold_on" @13:28:27.29 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @13:28:27.92 Lysis1.seek 0uL @13:28:27.96 Lysis2.seek 0uL @13:28:49.05 delay 15 seconds @13:29:04.07 SPR.command "hold_off" @13:29:07.14 Waiting up to 38:00 for SPR to enter state 7 @13:54:57.75 SPR analysis completed @13:55:01.83 Ended log spr23jul2211h.spr.bz2 @13:55:01.84 SPR.command "stats_off" @13:55:02.59 Handoff.select :close @13:55:02.74
Gate.power :heat,:OFF @13:55:02.76 Gate.power :cartridge,:OFF @13:55:02.86 PinPort.in :unlocking @13:55:02.92 PinPort.seek :clear @13:55:04.83 Carousel.in :default @13:55:04.97 Carousel.home.seek 40 @13:55:05.34 Plunge.seek :locked @13:55:05.52 Archive.in :unlocking @13:55:05.70 Archive.seek :clear @13:55:05.79 Air.in :unlocking @13:55:05.97 Air.seek :clear @13:55:06.08 Lysis1.in :unlocking @13:55:06.24 Lysis1.seek :clear @13:55:06.29 Lysis2.in :unlocking @13:55:06.43 Lysis2.seek :clear @13:55:11.03
Gate.power :motor,:OFF @13:55:11.05 Gate.power :core,:OFF @13:55:11.06 Gate.power :analytic1,:OFF @13:55:11.08 Gate.power :analytic2,:OFF @13:55:11.09 Gate.power :main,:OFF @13:55:11.10 Safely stopped and ready to power off @13:55:11.27 Cartridge::None in MAIN -- Not enough standard cartridges remain @18:05:02.66UTC06-Mar-24
Environmental Sample Processor @18:05:04.50 >BREAK< ESP3 Gateway v1.43 for core bus @18:05:04.53 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:05:08.48 Configuration for honu by nadia@mbari.org 7/31/23 /home/methane/esp2/type/auv/honu/configure.rb @18:05:09.24 Sea pressure (Abs) tare is 6% of full scale @18:05:12.01 Polling Can every 2:00 @18:05:12.05 Loading /home/methane/esp2/bin/processAll mission @18:05:12.77 Sea pressure (Abs) tare is 6% of full scale @18:05:12.79 Sampling default goal volumes @18:05:12.80 Options: {ExtraDilutions: [1,1,1]} Cartridge group: standard @18:05:12.97 Gate.power :sampler,:OFF,limit to 3500mA @18:05:12.98 Gate.power :analytic1,:ON,limit to 5000mA @18:05:12.99 Gate.power :analytic2,:ON,limit to 5000mA @18:05:13.69 Plunge.configure :default @18:05:13.76 Plunge.hold @18:05:13.85 SPR Module 1, FW Rev. B, FPGA Rev. C @18:05:13.88
Plunge.configure :default @18:05:14.01 SamplePump.configure :default @18:05:14.15 Toroid.configure :default @18:05:14.20 Toroid.hold @18:05:14.27 Toroid.configure :default @18:05:14.43 Carousel.configure :default @18:05:14.48 Carousel.hold @18:05:14.55 Carousel.configure :default @18:05:14.68 Pressure.configure :default @18:05:14.82 ExtraPump.configure :default @18:05:14.95 Archive.configure :default @18:05:15.00 Archive.hold @18:05:15.07 Archive.configure :default @18:05:15.20 Air.configure :default @18:05:15.25 Air.hold @18:05:15.32 Air.configure :default @18:05:15.43 Lysis1.configure :default @18:05:15.48 Lysis1.hold @18:05:15.55 Lysis1.configure :default @18:05:15.65 Lysis2.configure :default @18:05:15.70 Lysis2.hold @18:05:15.79 Lysis2.configure :default @18:05:15.89 PinPort.configure :default @18:05:15.94 PinPort.hold @18:05:16.01 PinPort.configure :default @18:05:16.07 All elf controllers running firmware version 4.91 @18:05:16.91 Toroid.seek :clear @18:05:17.07
Carousel.in :default @18:05:17.18 Carousel.home.seek 1 @18:05:17.42 Selecting Cartridge 54 @18:05:17.70 Carousel.seek 54 @18:05:28.80 Lysis1.in :fast @18:05:29.21 Lysis1.seek :locked @18:05:31.90 Plunge.seek :locked @18:05:32.06 Air.in :fast @18:05:32.11 Air.seek :locked @18:05:33.08
Carousel.reconfigure :locked @18:05:33.23 Powering cartridge bus @18:05:35.94 Gate.setIR 1750,5000,0,6 @18:05:36.06 Gate.setIR 0,5000,0,6 @18:05:36.12 PuckHeater.configure :default @18:05:36.19 Gate.power :heat,:ON,limit to 5000mA @18:05:36.87 Mounted lysis Cartridge @18:05:36.89 WARNING: Using default puck temperature sensor calibration @18:05:36.91 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:05:38.44 SPR.command "read_sequences" @18:05:38.50 SPR.command "stats_on" @18:05:39.37 SPR.command "start 0 1" @18:05:40.08 SPR.start /var/log/methane/spr24mar0618h.spr.bz2 @18:05:42.12 Waiting for baseline SPR stabilization @18:05:42.14 Handoff.select :waste @18:05:48.83 Syringe tare is 35% of full scale @18:05:48.86 PinPort.in :locking @18:05:48.91 PinPort.seek :locked @18:05:49.39 Archive.in :fast @18:05:49.46 Lysis2.in :fast @18:05:49.55 Lysis1.seek 1000uL @18:05:49.60 Lysis2.seek 1000uL @18:05:49.64 Archive.seek :full @18:05:54.19 delayUntil "18:15:41.69" @18:15:41.70 Lysis2.in :DAmix @18:15:41.81 Lysis1.in :DAmix @18:15:42.39 Lysis2.seek 750uL @18:15:42.46 Lysis1.seek 750uL @18:16:15.73 Lysis2.stop @18:16:15.84 Lysis1.stop @18:16:15.87 Slide::Error in Lysis2mixer -- Lysis Syringe #2 (LyS2) aborted at 762uL (sensed 774uL) @18:16:15.92 Thread::ChildDied in PROCESSING -- "Lysis2mixer" while stopping Lysis Syringe #1 (LyS1) @18:16:16.03 Lysis1.setRawPosition! -5000 @18:16:16.08 Slide::Error in PROCESSING -- Lysis Syringe #1 (LyS1) positionErr at 763uL (sensed 787uL) @18:16:16.10 Waiting to Resume from Checkpoint ""Lysis2mixer" while stopping Lysis Syringe #1 (LyS1)" @18:32:48.00UTC06-Mar-24
Environmental Sample Processor @18:32:49.82 >BREAK< ESP3 Gateway v1.43 for core bus @18:32:49.84 >BREAK< ESP3 Gateway v1.43 for cartridge bus @18:32:53.87 Configuration for honu by nadia@mbari.org 7/31/23 /home/methane/esp2/type/auv/honu/configure.rb @18:32:54.61 Sea pressure (Abs) tare is 6% of full scale @18:32:57.48 Polling Can every 2:00 @18:32:57.55 Loading /home/methane/esp2/bin/processAll mission @18:32:58.26 Sea pressure (Abs) tare is 6% of full scale @18:32:58.28 Sampling default goal volumes @18:32:58.30 Options: {ExtraDilutions: [1,1,1]} Cartridge group: standard @18:32:58.45 Gate.power :sampler,:OFF,limit to 3500mA @18:32:58.47 Gate.power :analytic1,:ON,limit to 5000mA @18:32:58.50 Gate.power :analytic2,:ON,limit to 5000mA @18:32:59.23 Plunge.configure :default @18:32:59.29 SPR Module 1, FW Rev. B, FPGA Rev. C @18:32:59.39
SamplePump.configure :default @18:32:59.56 Toroid.configure :default @18:32:59.60 Toroid.hold @18:32:59.67 Toroid.configure :default @18:32:59.82 Carousel.configure :default @18:32:59.87 Carousel.hold @18:32:59.94 Carousel.configure :default @18:33:00.07 Pressure.configure :default @18:33:00.20 ExtraPump.configure :default @18:33:00.33 Archive.configure :default @18:33:00.46 Air.configure :default @18:33:00.57 Lysis1.configure :default @18:33:00.68 Lysis2.configure :default @18:33:00.79 PinPort.configure :default @18:33:00.84 All elf controllers running firmware version 4.91 @18:33:01.66 Toroid.seek :clear @18:33:01.82
Carousel.in :default @18:33:01.93 Carousel.home.seek 54 @18:33:02.20 Selecting Cartridge 54 @18:33:02.46 Carousel.seek 54 @18:33:02.69 Lysis1.in :fast @18:33:02.74 Lysis1.seek :locked @18:33:05.35 Plunge.seek :locked @18:33:05.50 Air.in :fast @18:33:05.58 Air.seek :locked @18:33:06.54
Carousel.reconfigure :locked @18:33:06.69 Powering cartridge bus @18:33:09.75 Gate.setIR 1750,5000,0,6 @18:33:09.87 Gate.setIR 0,5000,0,6 @18:33:10.02 PuckHeater.configure :default @18:33:10.09 Gate.power :heat,:ON,limit to 5000mA @18:33:10.78 Mounted lysis Cartridge @18:33:10.81 WARNING: Using default puck temperature sensor calibration @18:33:10.82 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:33:12.37 SPR.command "read_sequences" @18:33:12.43 SPR.command "stats_on" @18:33:12.60 SPR.command "start 0 1" @18:33:13.30 SPR.start /var/log/methane/spr24mar0618h#2.spr.bz2 @18:33:15.81 Waiting for baseline SPR stabilization @18:33:15.83 Handoff.select :waste @18:33:22.53 Syringe tare is 35% of full scale @18:33:22.56 PinPort.in :locking @18:33:22.61 PinPort.seek :locked @18:33:23.12 Archive.in :fast @18:33:23.18 Lysis2.in :fast @18:33:23.24 Lysis1.seek 1000uL @18:33:23.41 Archive.seek :full @18:33:23.47 Lysis2.seek 1000uL @18:33:28.02 delayUntil "18:43:15.78" @18:43:15.79 Lysis2.in :DAmix @18:43:15.90 Lysis1.in :DAmix @18:43:16.39 Lysis2.seek 750uL @18:43:16.53 Lysis1.seek 750uL @18:43:51.57 delay 5 seconds @18:43:56.59 Handoff.select SPR @18:43:56.72 SPR.command "start 2 14" @18:43:57.52 SPR stabilized @18:43:57.69 delay 10 seconds @18:44:07.71 SPR.command "start 1 0" @18:44:10.87 Waiting up to 4:00 for SPR to enter state 1 @18:46:15.17 SPR.command "hold_on" @18:46:16.55 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:46:17.14 Lysis2.seek 450uL @18:46:17.21 Lysis1.seek 450uL @18:46:25.96 Lysis2.stop @18:46:26.02 Lysis1.stop @18:46:26.20 Lysis1.setRawPosition! -5322 @18:46:26.24 Slide::Error in Lysis2mixer -- Lysis Syringe #2 (LyS2) aborted at 688uL (sensed 705uL) @18:46:26.28 Thread::ChildDied in PROCESSING -- "Lysis2mixer" while setting Lysis Syringe #1 (LyS1)'s position @18:46:26.30 Slide::Error in PROCESSING -- Lysis Syringe #1 (LyS1) positionErr at 688uL (sensed 712uL) @18:46:26.32 Waiting to Resume from Checkpoint "Lysis Syringe #1 (LyS1) positionErr at 688uL (sensed 712uL)" @18:47:48.60 Session Begun @18:47:52.93 -> PP Handoff Pin Port (PP) cartridge @18:48:08.96 -> PP.legend {0 => ["clear",:home,:begin,:retract,:retracted,:unlocked], 77 => ["washStation",:wash], 2235 => ["cartridge",:end,:insert,:inserted,:locked]} @18:48:18.42 -> PP.jog 10 @18:48:18.45 PinPort.in :default @18:48:18.55 PinPort.jog 10 Handoff Pin Port (PP) at cartridge + 10 counts @18:48:26.24 -> PP.jog 10 @18:48:26.28 PinPort.jog 10 Handoff Pin Port (PP) at cartridge + 20 counts @18:48:37.29 -> PP.jog 10 @18:48:37.34 PinPort.jog 10 Handoff Pin Port (PP) at cartridge + 30 counts @18:48:39.96 -> PP.jog 10 @18:48:40.00 PinPort.jog 10 Handoff Pin Port (PP) at cartridge + 40 counts @18:48:43.20 -> PP.jog 10 @18:48:43.24 PinPort.jog 10 Handoff Pin Port (PP) at cartridge + 50 counts @18:48:52.40 -> Handoff Handoff Valve::Manifold selects Analytical Module @18:48:57.70 -> Handoff.waste @18:48:57.75 Handoff.select :waste Handoff Valve::Manifold selects waste @18:49:18.25 -> LyS1 @18:49:18.36 Slide::Error in bill -- Lysis Syringe #1 (LyS1) Not yet Homed! @18:49:25.53 -> LyS1.legend {-11650 => "extended", -8365 => ["empty",0,:end], -1750 => ["locked",:lock], -970 => ["full",1730,:cartridge], 0 => ["clear",:home,:begin], 50 => :limit} @18:50:24.30 -> LyS1.jog -50 @18:50:24.33 Lysis1.in :default @18:50:24.44 Lysis1.jog -50 @18:50:25.02 Slide::Error in bill -- Lysis Syringe #1 (LyS1) Not yet Homed! @18:50:37.21 -> LyS1.goHome! @18:50:37.26 Lysis1.in :default @18:50:37.34 Lysis1.home.jog 0 @18:50:37.57 Lysis1.in :homing @18:50:37.65 Lysis1.jog 6270 @18:50:41.12 Lysis1.setRawPosition! 50 @18:50:41.19 Lysis1.seek :home @18:50:41.50 Lysis1.in :default Lysis Syringe #1 (LyS1) clear @18:50:55.26 -> LyS1.to 1000 @18:50:55.31 Lysis1.seek 1000uL Lysis Syringe #1 (LyS1) at 1000uL @18:51:37.34 -> LyS1.to 800 @18:51:37.39 Lysis1.seek 800uL Lysis Syringe #1 (LyS1) at 800uL @18:51:57.62 -> LyS1.to 700 @18:51:57.68 Lysis1.seek 700uL @18:51:58.82 Slide::Error in bill -- Lysis Syringe #1 (LyS1) positionErr at 710uL (sensed 738uL) @18:52:11.26 -> PP Handoff Pin Port (PP) at cartridge + 50 counts @18:53:41.52 -> Cartridge.unlock @18:53:41.63 Gate.power :heat,:OFF @18:53:41.65 Gate.power :cartridge,:OFF @18:53:41.79 PinPort.in :unlocking @18:53:41.87 PinPort.seek :clear @18:53:42.37 Carousel.in :default @18:53:42.53 Carousel.home.seek 54 @18:53:42.91 Archive.in :unlocking @18:53:43.07 Archive.seek :clear @18:53:43.16 Air.in :unlocking @18:53:43.32 Air.seek :clear @18:53:43.50 Lysis2.in :unlocking @18:53:43.63 Lysis2.seek :clear @18:53:46.80 Slide::Error in bill -- Lysis Syringe #1 (LyS1) Not yet Homed! @18:54:04.21 -> SC.to 60 @18:54:04.26 Carousel.seek 60 @18:54:04.53 Slide::Error in bill -- Storage Carousel (SC) positionErr at 54 + 103 counts (sensed 54 + 16 counts) @18:54:33.45 -> SC.to 56 @18:54:33.50 Carousel.seek 56 @18:54:33.68 Slide::Error in bill -- Storage Carousel (SC) notReady at 55 - 81 counts (sensed 54 + 6 counts) @18:54:47.29 -> ESP.ready! @18:54:48.13 Carousel.in :default @18:54:48.18 Lysis1.in :default @18:54:48.29 Carousel.home.jog 0 @18:54:48.37 Lysis1.home.jog 0 @18:54:48.65 Lysis1.in :homing @18:54:48.74 Lysis1.jog 6154 @18:54:52.17 Lysis1.setRawPosition! 50 @18:54:52.25 Lysis1.seek :home @18:54:52.56 Lysis1.in :default @18:54:52.93 Toroid.seek :clear @18:54:53.12 Carousel.in :default @18:54:53.27 Carousel.home.seek 54 Plunge = Plunge Valve (PV) locked Handoff Valve::Manifold selects waste SamplePump = Sample Pump (SP) Not yet Homed! Intake is in an unknown state Exhaust is in an unknown state Bypass = Bypass (PB) is in an unknown state Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 54 Pressure = Absolute Pressure (Leak) Sensor @14psia Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @18:54:59.83 -> SC.to 60 @18:54:59.86 Carousel.seek 60 @18:55:00.15 Slide::Error in bill -- Storage Carousel (SC) positionErr at 54 + 117 counts (sensed 54 + 20 counts) @18:55:26.68 -> MainThread.exit # @18:55:31.60 -> eit :eit @18:55:33.94 -> exit @18:55:33.99 Session Ended @18:55:36.31 Ended log spr24mar0618h#2.spr.bz2 @18:55:36.32
SPR.command "stats_off" @18:55:36.56 Intake.close @18:55:36.64 Exhaust.close @18:55:36.66 Bypass.close @18:55:36.68 Handoff.select :close nohup: procesAll: No such file or directory nohup: procesAll: No such file or directory @21:31:41.47UTC06-Mar-24
Environmental Sample Processor @21:31:43.27 >BREAK< ESP3 Gateway v1.43 for core bus @21:31:43.29 >BREAK< ESP3 Gateway v1.43 for cartridge bus @21:31:47.11 Configuration for honu by nadia@mbari.org 7/31/23 /home/methane/esp2/type/auv/honu/configure.rb @21:31:47.85 Sea pressure (Abs) tare is 6% of full scale @21:31:50.64 Polling Can every 2:00 @21:31:50.69 Loading /home/methane/esp2/bin/processAll mission @21:31:51.34 Sea pressure (Abs) tare is 6% of full scale @21:31:51.35 Sampling default goal volumes @21:31:51.37 Options: {ExtraDilutions: [1,1,1]} Cartridge group: standard @21:31:51.56 Gate.power :sampler,:OFF,limit to 3500mA @21:31:51.57 Gate.power :analytic1,:ON,limit to 5000mA @21:31:51.58 Gate.power :analytic2,:ON,limit to 5000mA @21:31:52.31 Plunge.configure :default @21:31:52.36 Plunge.hold @21:31:52.43 Plunge.configure :default @21:31:52.59 SPR Module 1, FW Rev. B, FPGA Rev. C @21:31:52.62
SamplePump.configure :default @21:31:52.75 Toroid.configure :default @21:31:52.80 Toroid.hold @21:31:52.87 Toroid.configure :default @21:31:53.01 Carousel.configure :default @21:31:53.07 Carousel.hold @21:31:53.14 Carousel.configure :default @21:31:53.27 Pressure.configure :default @21:31:53.41 ExtraPump.configure :default @21:31:53.57 Archive.configure :default @21:31:53.62 Archive.hold @21:31:53.69 Archive.configure :default @21:31:53.82 Air.configure :default @21:31:53.86 Air.hold @21:31:53.93 Air.configure :default @21:31:54.04 Lysis1.configure :default @21:31:54.09 Lysis1.hold @21:31:54.16 Lysis1.configure :default @21:31:54.27 Lysis2.configure :default @21:31:54.33 Lysis2.hold @21:31:54.40 Lysis2.configure :default @21:31:54.50 PinPort.configure :default @21:31:54.55 PinPort.hold @21:31:54.62 PinPort.configure :default @21:31:54.67 All elf controllers running firmware version 4.91 @21:31:55.41 Toroid.seek :clear @21:31:55.60
Carousel.in :default @21:31:55.71 Carousel.home.seek 60 @21:31:55.97 Selecting Cartridge 54 @21:31:56.25 Carousel.seek 54 @21:31:57.80 Lysis1.in :fast @21:31:58.29 Lysis1.seek :locked @21:32:00.93 Plunge.seek :locked @21:32:01.09 Air.in :fast @21:32:01.15 Air.seek :locked @21:32:02.12
Carousel.reconfigure :locked @21:32:02.26 Powering cartridge bus @21:32:04.93 Gate.setIR 1750,5000,0,6 @21:32:05.06 Gate.setIR 0,5000,0,6 @21:32:05.14 PuckHeater.configure :default @21:32:05.20 Gate.power :heat,:ON,limit to 5000mA @21:32:05.88 Mounted lysis Cartridge @21:32:05.90 WARNING: Using default puck temperature sensor calibration @21:32:05.92 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:32:07.44 SPR.command "read_sequences" @21:32:07.63 SPR.command "stats_on" @21:32:07.78 SPR.command "start 0 1" @21:32:08.49 SPR.start /var/log/methane/spr24mar0621h.spr.bz2 @21:32:10.48 Waiting for baseline SPR stabilization @21:32:10.50 Handoff.select :waste @21:32:17.19 Syringe tare is 35% of full scale @21:32:17.22 PinPort.in :locking @21:32:17.27 PinPort.seek :locked @21:32:17.75 Archive.in :fast @21:32:17.83 Lysis2.in :fast @21:32:17.90 Lysis1.seek 1000uL @21:32:17.99 Archive.seek :full @21:32:18.10 Lysis2.seek 1000uL @21:32:22.66 delayUntil "21:42:10.05" @21:42:10.07 Lysis2.in :DAmix @21:42:10.18 Lysis1.in :DAmix @21:42:10.73 Lysis2.seek 750uL @21:42:10.79 Lysis1.seek 750uL @21:42:45.87 delay 5 seconds @21:42:50.89 Handoff.select SPR @21:42:51.02 SPR.command "start 2 14" @21:42:51.76 SPR stabilized @21:42:51.91 delay 10 seconds @21:43:01.93 SPR.command "start 1 0" @21:43:05.63 Waiting up to 4:00 for SPR to enter state 1 @21:45:42.41 Session Begun @21:45:50.38 -> MainThread.exit # @21:45:57.29 Ended log spr24mar0621h.spr.bz2 @21:45:57.31
SPR.command "stats_off" @21:45:58.16 Intake.close @21:45:58.68 Exhaust.close @21:45:58.73 Bypass.close @21:45:58.78 Handoff.select :close @21:45:59.20 Session Ended @22:03:36.05UTC06-Mar-24
Environmental Sample Processor @22:03:37.84 >BREAK< ESP3 Gateway v1.43 for core bus @22:03:37.86 >BREAK< ESP3 Gateway v1.43 for cartridge bus @22:03:41.74 Configuration for honu by nadia@mbari.org 7/31/23 /home/methane/esp2/type/auv/honu/configure.rb @22:03:42.49 Sea pressure (Abs) tare is 6% of full scale @22:03:45.32 Polling Can every 2:00 @22:03:45.37 Loading /home/methane/esp2/bin/processAll mission @22:03:46.03 Sea pressure (Abs) tare is 6% of full scale @22:03:46.04 Sampling default goal volumes @22:03:46.06 Options: {ExtraDilutions: [1,1,1]} Cartridge group: standard @22:03:46.24 Gate.power :sampler,:OFF,limit to 3500mA @22:03:46.26 Gate.power :analytic1,:ON,limit to 5000mA @22:03:46.27 Gate.power :analytic2,:ON,limit to 5000mA @22:03:46.85 SPR Module 1, FW Rev. B, FPGA Rev. C @22:03:47.02
Plunge.configure :default @22:03:47.16 SamplePump.configure :default @22:03:47.28 Toroid.configure :default @22:03:47.33 Toroid.hold @22:03:47.40 Toroid.configure :default @22:03:47.53 Carousel.configure :default @22:03:47.58 Carousel.hold @22:03:47.65 Carousel.configure :default @22:03:47.79 Pressure.configure :default @22:03:47.92 ExtraPump.configure :default @22:03:48.06 Archive.configure :default @22:03:48.24 Air.configure :default @22:03:48.35 Lysis1.configure :default @22:03:48.45 Lysis2.configure :default @22:03:48.56 PinPort.configure :default @22:03:48.62 All elf controllers running firmware version 4.91 @22:03:49.29 Toroid.seek :clear @22:03:49.45
Carousel.in :default @22:03:49.57 Carousel.home.seek 58 @22:03:49.83 Selecting Cartridge 54 @22:03:50.10 Carousel.seek 54 @22:03:51.22 Lysis1.in :fast @22:03:51.27 Lysis1.seek :locked @22:03:53.89 Plunge.seek :locked @22:03:54.03 Air.in :fast @22:03:54.09 Air.seek :locked @22:03:55.05
Carousel.reconfigure :locked @22:03:55.19 Powering cartridge bus @22:03:57.99 Gate.setIR 1750,5000,0,6 @22:03:58.11 Gate.setIR 0,5000,0,6 @22:03:58.28 PuckHeater.configure :default @22:03:58.34 Gate.power :heat,:ON,limit to 5000mA @22:03:59.03 Mounted lysis Cartridge @22:03:59.05 WARNING: Using default puck temperature sensor calibration @22:03:59.07 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:04:00.58 SPR.command "read_sequences" @22:04:00.88 SPR.command "stats_on" @22:04:01.04 SPR.command "start 0 1" @22:04:01.74 SPR.start /var/log/methane/spr24mar0622h.spr.bz2 @22:04:03.73 Waiting for baseline SPR stabilization @22:04:03.75 Handoff.select :waste @22:04:10.44 Syringe tare is 35% of full scale @22:04:10.47 PinPort.in :locking @22:04:10.52 PinPort.seek :locked @22:04:10.99 Archive.in :fast @22:04:11.08 Lysis2.in :fast @22:04:11.15 Lysis1.seek 1000uL @22:04:11.20 Archive.seek :full @22:04:11.33 Lysis2.seek 1000uL @22:04:15.85 delayUntil "22:14:03.30" @22:14:03.31 Lysis2.in :DAmix @22:14:03.42 Lysis1.in :DAmix @22:14:03.94 Lysis2.seek 750uL @22:14:04.02 Lysis1.seek 750uL @22:14:39.11 delay 5 seconds @22:14:44.13 Handoff.select SPR @22:14:44.27 SPR.command "start 2 14" @22:14:45.01 SPR stabilized @22:14:45.16 delay 10 seconds @22:14:55.18 SPR.command "start 1 0" @22:14:58.90 Waiting up to 4:00 for SPR to enter state 1 @22:17:03.62 SPR.command "hold_on" @22:17:05.52 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @22:17:06.08 Lysis2.seek 450uL @22:17:06.20 Lysis1.seek 450uL @22:17:48.23 delay 15 seconds @22:18:03.25 SPR.command "hold_off" @22:18:06.59 Waiting up to 1:00:00 for SPR to enter state 7 @23:02:10.62 SPRsummary:-79.1RIU,62.5RIU,152RIU,206RIU after 3686s @23:08:47.97 delay 10 seconds @23:08:57.99 SPR.command "start 1 0" @23:09:00.69 Waiting up to 4:00 for SPR to enter state 1 @23:11:04.98 SPR.command "hold_on" @23:11:06.37 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @23:11:06.99 Lysis2.seek 300uL @23:11:07.05 Lysis1.seek 300uL @23:11:28.14 delay 15 seconds @23:11:43.17 SPR.command "hold_off" @23:11:46.24 Waiting up to 1:00:00 for SPR to enter state 7 @00:02:28.54UTC07-Mar-24 delay 10 seconds @00:02:38.56 SPR.command "start 1 0" @00:02:41.26 Waiting up to 4:00 for SPR to enter state 1 @00:04:45.55 SPR.command "hold_on" @00:04:46.93 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @00:04:47.59 Lysis2.seek 150uL @00:04:47.68 Lysis1.seek 150uL @00:05:08.72 delay 15 seconds @00:05:23.74 SPR.command "hold_off" @00:05:26.78 Waiting up to 1:00:00 for SPR to enter state 7 @00:34:30.80 Session Begun @00:34:36.03 -> showlog @00:56:08.96 SPR analysis completed @00:56:16.73 Ended log spr24mar0622h.spr.bz2 @00:56:16.75 SPR.command "stats_off" @00:56:17.12 Intake.close @00:56:17.22 Exhaust.close @00:56:17.29 Bypass.close @00:56:17.42 Handoff.select :close @00:56:17.62
Gate.power :heat,:OFF @00:56:17.64 Gate.power :cartridge,:OFF @00:56:17.76 PinPort.in :unlocking @00:56:17.84 PinPort.seek :clear @00:56:18.32 Carousel.in :default @00:56:18.45 Carousel.home.seek 54 @00:56:19.24 Plunge.seek :locked @00:56:19.44 Archive.in :unlocking @00:56:19.61 Archive.seek :clear @00:56:19.66 Air.in :unlocking @00:56:19.91 Air.seek :clear @00:56:19.99 Lysis1.in :unlocking @00:56:20.24 Lysis1.seek :clear @00:56:20.38 Lysis2.in :unlocking @00:56:20.48 Lysis2.seek :clear @00:56:24.80
Gate.power :motor,:OFF @00:56:24.83 Gate.power :core,:OFF @00:56:24.86 Gate.power :analytic1,:OFF @00:56:24.89 Gate.power :analytic2,:OFF @00:56:24.94 Gate.power :main,:OFF @00:56:24.98 Safely stopped and ready to power off @00:56:25.54 Gate.power :sampler,:OFF,limit to 3500mA @00:56:28.67 Gate.power :main,:ON,limit to 5000mA @00:56:28.69 Gate.power :analytic1,:ON,limit to 5000mA @00:56:28.73 Gate.power :analytic2,:ON,limit to 5000mA @00:56:28.85 Powering core bus @00:56:31.18 SPR Module 1, FW Rev. B, FPGA Rev. C @00:56:31.62
Plunge.configure :default @00:56:31.73 SamplePump.configure :default @00:56:31.83 Toroid.configure :default @00:56:31.94 Carousel.configure :default @00:56:32.04 Pressure.configure :default @00:56:32.15 ExtraPump.configure :default @00:56:32.29 Archive.configure :default @00:56:32.40 Air.configure :default @00:56:32.48 Lysis1.configure :default @00:56:32.56 Lysis2.configure :default @00:56:32.65 PinPort.configure :default @00:56:32.72 All elf controllers running firmware version 4.91 @00:56:32.75 Gate.power :motor,:ON,limit to 5000mA @00:56:33.06 Plunge.home.jog 0 @00:56:33.80 Lysis2.home.jog 0 @00:56:33.84 Lysis1.home.jog 0 @00:56:33.91 Archive.home.jog 0 @00:56:33.96 Carousel.home.jog 0 @00:56:34.01 Air.home.jog 0 @00:56:34.05 Toroid.home.jog 0 @00:56:34.08 PinPort.home.jog 0 @00:56:35.05 Plunge.in :homing @00:56:35.29 Plunge.jog 1532 @00:56:35.36 PinPort.in :homing @00:56:35.42 Lysis1.in :homing @00:56:35.46 Air.in :homing @00:56:35.49 Toroid.in :homing @00:56:35.53 Archive.in :homing @00:56:35.58 Lysis2.in :homing @00:56:35.93 PinPort.jog -104 @00:56:35.99 Lysis2.jog 1390 @00:56:36.03 Air.jog 1358 @00:56:36.06 Toroid.jog 595 @00:56:36.10 Lysis1.jog 1388 @00:56:36.21 Archive.jog 1383 @00:56:37.25 PinPort.setRawPosition! 0 @00:56:37.27 Plunge.setRawPosition! 50 @00:56:37.41 Plunge.seek :home @00:56:37.45 PinPort.seek :home @00:56:37.69 Lysis2.setRawPosition! 50 @00:56:37.72 PinPort.in :default @00:56:37.84 Lysis2.seek :home @00:56:37.98 Plunge.in :default @00:56:38.12 Lysis1.setRawPosition! 50 @00:56:38.18 Air.setRawPosition! 50 @00:56:38.25 Archive.setRawPosition! 50 @00:56:38.42 Lysis1.seek :home @00:56:38.47 Air.seek :home @00:56:38.58 Archive.seek :home @00:56:38.80 Lysis2.in :default @00:56:38.97 Air.in :default @00:56:39.02 Archive.in :default @00:56:39.06 Lysis1.in :default @00:56:39.10 Toroid.setRawPosition! 0 @00:56:39.33 Toroid.seek :home @00:56:39.45 Toroid.in :default @00:56:39.60 Toroid.seek :clear @00:56:41.34
Carousel.in :default @00:56:41.48 Carousel.home.seek 54 @00:56:41.78 Selecting Cartridge 53 @00:56:42.09 Carousel.seek 53 @00:56:42.63 Lysis1.in :fast @00:56:42.71 Lysis1.seek :locked @00:56:45.36 Plunge.seek :locked @00:56:45.55 Air.in :fast @00:56:45.62 Air.seek :locked @00:56:46.62
Carousel.reconfigure :locked @00:56:46.79 Powering cartridge bus @00:56:49.44 Gate.setIR 1750,5000,0,6 @00:56:49.57 Gate.setIR 0,5000,0,6 @00:56:49.66 PuckHeater.configure :default @00:56:49.75 Gate.power :heat,:ON,limit to 5000mA @00:56:50.36 Mounted lysis Cartridge @00:56:50.41 WARNING: Using default puck temperature sensor calibration @00:56:50.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:56:51.98 SPR.command "read_sequences" @00:56:52.79 SPR.command "stats_on" @00:56:52.95 SPR.command "start 0 1" @00:56:53.66 SPR.start /var/log/methane/spr24mar0700h.spr.bz2 @00:56:56.19 Waiting for baseline SPR stabilization @00:56:56.22 Handoff.select :waste @00:57:02.96 Syringe tare is 35% of full scale @00:57:02.99 PinPort.in :locking @00:57:03.09 PinPort.seek :locked @00:57:03.62 Archive.in :fast @00:57:03.72 Lysis2.in :fast @00:57:03.81 Lysis1.seek 1000uL @00:57:03.85 Archive.seek :full @00:57:04.04 Lysis2.seek 1000uL @00:57:08.61 delayUntil "01:06:56.13" @01:06:56.15 Lysis2.in :DAmix @01:06:56.32 Lysis1.in :DAmix @01:06:56.86 Lysis2.seek 750uL @01:06:56.89 Lysis1.seek 750uL @01:07:32.05 delay 5 seconds @01:07:37.07 Handoff.select SPR @01:07:37.22 SPR.command "start 2 14" @01:07:37.86 SPR stabilized @01:07:38.03 delay 10 seconds @01:07:48.05 SPR.command "start 1 0" @01:07:51.23 Waiting up to 4:00 for SPR to enter state 1 @01:09:55.52 SPR.command "hold_on" @01:09:56.91 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @01:09:57.52 Lysis2.seek 450uL @01:09:57.59 Lysis1.seek 450uL @01:10:39.68 delay 15 seconds @01:10:54.70 SPR.command "hold_off" @01:10:57.56 Waiting up to 1:00:00 for SPR to enter state 7 @01:11:15.14 Session Ended @01:31:23.74 Session Begun @01:31:28.68 -> exit @01:31:28.70 Session Ended @01:50:12.89 Session Begun @01:50:14.57 -> slot {52..44 => { Type: :daSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 53 => { Type: :daSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr24mar0700h.spr.bz2}, 54 => { Type: :daSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr24mar0622h.spr.bz2}} @01:50:50.19 -> showlog @01:50:56.90 -> showlog -50 @01:55:02.53 SPRsummary:-25.1RIU,125RIU,178RIU,255RIU after 3686s @02:01:39.90 delay 10 seconds @02:01:49.92 SPR.command "start 1 0" @02:01:53.13 Waiting up to 4:00 for SPR to enter state 1 @02:03:57.85 SPR.command "hold_on" @02:03:59.23 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:03:59.88 Lysis2.seek 300uL @02:03:59.93 Lysis1.seek 300uL @02:04:21.07 delay 15 seconds @02:04:36.09 SPR.command "hold_off" @02:04:39.07 Waiting up to 1:00:00 for SPR to enter state 7 @02:14:16.86 Session Ended @02:55:21.40 delay 10 seconds @02:55:31.42 SPR.command "start 1 0" @02:55:34.64 Waiting up to 4:00 for SPR to enter state 1 @02:57:39.36 SPR.command "hold_on" @02:57:40.74 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:57:41.37 Lysis2.seek 150uL @02:57:41.43 Lysis1.seek 150uL @02:58:02.53 delay 15 seconds @02:58:17.55 SPR.command "hold_off" @02:58:20.58 Waiting up to 1:00:00 for SPR to enter state 7 @03:49:02.77 SPR analysis completed @03:49:10.45 Ended log spr24mar0700h.spr.bz2 @03:49:10.47 SPR.command "stats_off" @03:49:10.94 Handoff.select :close @03:49:11.09
Gate.power :heat,:OFF @03:49:11.10 Gate.power :cartridge,:OFF @03:49:11.18 PinPort.in :unlocking @03:49:11.23 PinPort.seek :clear @03:49:11.69 Carousel.in :default @03:49:11.80 Carousel.home.seek 53 @03:49:12.11 Plunge.seek :locked @03:49:12.74 Archive.in :unlocking @03:49:12.85 Archive.seek :clear @03:49:12.96 Air.in :unlocking @03:49:13.08 Air.seek :clear @03:49:13.19 Lysis1.in :unlocking @03:49:13.32 Lysis1.seek :clear @03:49:13.40 Lysis2.in :unlocking @03:49:13.48 Lysis2.seek :clear @03:49:17.77
Gate.power :motor,:OFF @03:49:17.78 Gate.power :core,:OFF @03:49:17.80 Gate.power :analytic1,:OFF @03:49:17.81 Gate.power :analytic2,:OFF @03:49:17.82 Gate.power :main,:OFF @03:49:17.83 Safely stopped and ready to power off @03:49:18.33 Gate.power :sampler,:OFF,limit to 3500mA @03:49:21.61 Gate.power :main,:ON,limit to 5000mA @03:49:21.62 Gate.power :analytic1,:ON,limit to 5000mA @03:49:21.64 Gate.power :analytic2,:ON,limit to 5000mA @03:49:21.73 Powering core bus @03:49:24.07 SPR Module 1, FW Rev. B, FPGA Rev. C @03:49:24.50
Plunge.configure :default @03:49:24.58 SamplePump.configure :default @03:49:24.65 Toroid.configure :default @03:49:24.73 Carousel.configure :default @03:49:24.81 Pressure.configure :default @03:49:24.88 ExtraPump.configure :default @03:49:24.96 Archive.configure :default @03:49:25.03 Air.configure :default @03:49:25.08 Lysis1.configure :default @03:49:25.15 Lysis2.configure :default @03:49:25.20 PinPort.configure :default @03:49:25.25 All elf controllers running firmware version 4.91 @03:49:25.27 Gate.power :motor,:ON,limit to 5000mA @03:49:25.43 Plunge.home.jog 0 @03:49:26.23 Lysis2.home.jog 0 @03:49:26.28 Air.home.jog 0 @03:49:26.32 Carousel.home.jog 0 @03:49:26.34 Lysis1.home.jog 0 @03:49:26.37 Archive.home.jog 0 @03:49:26.40 Toroid.home.jog 0 @03:49:26.43 PinPort.home.jog 0 @03:49:27.17 Plunge.in :homing @03:49:27.42 Plunge.jog 1561 @03:49:27.60 Lysis2.in :homing @03:49:27.66 Lysis1.in :homing @03:49:27.68 Archive.in :homing @03:49:27.71 PinPort.in :homing @03:49:27.73 Toroid.in :homing @03:49:27.77 Air.in :homing @03:49:28.00 Lysis2.jog 1394 @03:49:28.04 Lysis1.jog 1384 @03:49:28.08 Archive.jog 1387 @03:49:28.10 PinPort.jog -103 @03:49:28.16 Toroid.jog 618 @03:49:28.26 Air.jog 1361 @03:49:28.65 PinPort.setRawPosition! 0 @03:49:28.70 PinPort.seek :home @03:49:29.25 PinPort.in :default @03:49:29.27 Plunge.setRawPosition! 50 @03:49:29.37 Plunge.seek :home @03:49:29.46 Lysis2.setRawPosition! 50 @03:49:29.52 Lysis2.seek :home @03:49:29.63 Lysis1.setRawPosition! 50 @03:49:29.66 Archive.setRawPosition! 50 @03:49:29.74 Lysis1.seek :home @03:49:29.78 Archive.seek :home @03:49:30.06 Air.setRawPosition! 50 @03:49:30.09 Plunge.in :default @03:49:30.11 Lysis2.in :default @03:49:30.31 Air.seek :home @03:49:30.43 Lysis1.in :default @03:49:30.48 Archive.in :default @03:49:30.61 Toroid.setRawPosition! 0 @03:49:30.66 Toroid.seek :home @03:49:30.78 Toroid.in :default @03:49:30.81 Air.in :default @03:49:31.17 Toroid.seek :clear @03:49:32.88
Carousel.in :default @03:49:33.00 Carousel.home.seek 53 @03:49:33.26 Selecting Cartridge 52 @03:49:33.54 Carousel.seek 52 @03:49:34.05 Lysis1.in :fast @03:49:34.10 Lysis1.seek :locked @03:49:36.71 Plunge.seek :locked @03:49:36.97 Air.in :fast @03:49:37.02 Air.seek :locked @03:49:37.99
Carousel.reconfigure :locked @03:49:38.13 Powering cartridge bus @03:49:40.78 Gate.setIR 1750,5000,0,6 @03:49:40.90 Gate.setIR 0,5000,0,6 @03:49:40.97 PuckHeater.configure :default @03:49:41.02 Gate.power :heat,:ON,limit to 5000mA @03:49:41.72 Mounted lysis Cartridge board#Q12558 @03:49:41.75 WARNING: Using default puck temperature sensor calibration @03:49:41.76 Using default cartridge heat loss curve for HeatingAccelInCarousel @03:49:43.29 SPR.command "read_sequences" @03:49:43.81 SPR.command "stats_on" @03:49:43.95 SPR.command "start 0 1" @03:49:44.67 SPR.start /var/log/methane/spr24mar0703h.spr.bz2 @03:49:46.66 Waiting for baseline SPR stabilization @03:49:46.68 Handoff.select :waste @03:49:53.38 Syringe tare is 35% of full scale @03:49:53.40 PinPort.in :locking @03:49:53.45 PinPort.seek :locked @03:49:53.93 Archive.in :fast @03:49:53.98 Lysis2.in :fast @03:49:54.07 Lysis1.seek 1000uL @03:49:54.20 Archive.seek :full @03:49:54.30 Lysis2.seek 1000uL @03:49:58.82 delayUntil "03:59:46.24" @03:59:46.25 Lysis2.in :DAmix @03:59:46.36 Lysis1.in :DAmix @03:59:46.84 Lysis2.seek 750uL @03:59:46.92 Lysis1.seek 750uL @04:00:21.99 delay 5 seconds @04:00:27.01 Handoff.select SPR @04:00:27.13 SPR.command "start 2 14" @04:00:27.94 SPR stabilized @04:00:28.08 delay 10 seconds @04:00:38.10 SPR.command "start 1 0" @04:00:41.31 Waiting up to 4:00 for SPR to enter state 1 @04:02:45.60 SPR.command "hold_on" @04:02:46.98 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @04:02:47.57 Lysis2.seek 450uL @04:02:47.63 Lysis1.seek 450uL @04:03:29.66 delay 15 seconds @04:03:44.68 SPR.command "hold_off" @04:03:47.63 Waiting up to 1:00:00 for SPR to enter state 7 @04:47:52.61 SPRsummary:36.5RIU,39.3RIU,2.65RIU,2.88RIU after 3685s @04:54:29.95 delay 10 seconds @04:54:39.97 SPR.command "start 1 0" @04:54:42.68 Waiting up to 4:00 for SPR to enter state 1 @04:56:46.97 SPR.command "hold_on" @04:56:48.35 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:56:49.05 Lysis2.seek 300uL @04:56:49.10 Lysis1.seek 300uL @04:57:10.23 delay 15 seconds @04:57:25.25 SPR.command "hold_off" @04:57:28.72 Waiting up to 1:00:00 for SPR to enter state 7 @05:48:11.47 delay 10 seconds @05:48:21.49 SPR.command "start 1 0" @05:48:24.21 Waiting up to 4:00 for SPR to enter state 1 @05:50:28.48 SPR.command "hold_on" @05:50:30.38 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @05:50:31.00 Lysis2.seek 150uL @05:50:31.08 Lysis1.seek 150uL @05:50:52.14 delay 15 seconds @05:51:07.31 SPR.command "hold_off" @05:51:10.64 Waiting up to 1:00:00 for SPR to enter state 7 @06:41:51.90 SPR analysis completed @06:41:59.53 Ended log spr24mar0703h.spr.bz2 @06:41:59.55 SPR.command "stats_off" @06:42:00.06 Handoff.select :close @06:42:00.22
Gate.power :heat,:OFF @06:42:00.23 Gate.power :cartridge,:OFF @06:42:00.31 PinPort.in :unlocking @06:42:00.36 PinPort.seek :clear @06:42:00.82 Carousel.in :default @06:42:00.92 Carousel.home.seek 52 @06:42:01.24 Plunge.seek :locked @06:42:01.40 Archive.in :unlocking @06:42:01.51 Archive.seek :clear @06:42:01.61 Air.in :unlocking @06:42:01.72 Air.seek :clear @06:42:01.83 Lysis1.in :unlocking @06:42:01.96 Lysis1.seek :clear @06:42:02.02 Lysis2.in :unlocking @06:42:02.14 Lysis2.seek :clear @06:42:06.45
Gate.power :motor,:OFF @06:42:06.46 Gate.power :core,:OFF @06:42:06.48 Gate.power :analytic1,:OFF @06:42:06.49 Gate.power :analytic2,:OFF @06:42:06.50 Gate.power :main,:OFF @06:42:06.51 Safely stopped and ready to power off @06:42:06.72 Gate.power :sampler,:OFF,limit to 3500mA @06:42:10.29 Gate.power :main,:ON,limit to 5000mA @06:42:10.30 Gate.power :analytic1,:ON,limit to 5000mA @06:42:10.31 Gate.power :analytic2,:ON,limit to 5000mA @06:42:10.40 Powering core bus @06:42:13.32 Plunge.configure :default @06:42:13.40 SamplePump.configure :default @06:42:13.48 Toroid.configure :default @06:42:13.55 Carousel.configure :default @06:42:13.66 SPR Module 1, FW Rev. B, FPGA Rev. C @06:42:13.68
Pressure.configure :default @06:42:13.75 ExtraPump.configure :default @06:42:13.83 Archive.configure :default @06:42:13.90 Air.configure :default @06:42:13.95 Lysis1.configure :default @06:42:14.01 Lysis2.configure :default @06:42:14.06 PinPort.configure :default @06:42:14.11 All elf controllers running firmware version 4.91 @06:42:14.13 Gate.power :motor,:ON,limit to 5000mA @06:42:14.44 Plunge.home.jog 0 @06:42:15.04 Lysis2.home.jog 0 @06:42:15.11 Lysis1.home.jog 0 @06:42:15.15 Archive.home.jog 0 @06:42:15.18 Carousel.home.jog 0 @06:42:15.24 Air.home.jog 0 @06:42:15.29 Toroid.home.jog 0 @06:42:15.31 PinPort.home.jog 0 @06:42:16.03 Plunge.in :homing @06:42:16.32 Plunge.jog 1554 @06:42:16.50 PinPort.in :homing @06:42:16.56 Lysis2.in :homing @06:42:16.59 Air.in :homing @06:42:16.61 Toroid.in :homing @06:42:16.63 Lysis1.in :homing @06:42:16.65 Archive.in :homing @06:42:16.88 PinPort.jog -102 @06:42:16.94 Lysis2.jog 1393 @06:42:16.98 Lysis1.jog 1384 @06:42:17.00 Archive.jog 1387 @06:42:17.02 Toroid.jog 619 @06:42:17.07 Air.jog 1364 @06:42:17.46 PinPort.setRawPosition! 0 @06:42:17.51 PinPort.seek :home @06:42:17.60 Plunge.setRawPosition! 50 @06:42:17.66 PinPort.in :default @06:42:17.75 Plunge.seek :home @06:42:18.03 Plunge.in :default @06:42:18.36 Lysis2.setRawPosition! 50 @06:42:18.39 Lysis1.setRawPosition! 50 @06:42:18.49 Lysis2.seek :home @06:42:18.53 Lysis1.seek :home @06:42:18.69 Air.setRawPosition! 50 @06:42:18.71 Archive.setRawPosition! 50 @06:42:18.82 Air.seek :home @06:42:18.85 Archive.seek :home @06:42:19.00 Lysis1.in :default @06:42:19.04 Lysis2.in :default @06:42:19.18 Air.in :default @06:42:19.25 Archive.in :default @06:42:19.71 Toroid.setRawPosition! 0 @06:42:19.86 Toroid.seek :home @06:42:19.98 Toroid.in :default @06:42:20.06 Toroid.seek :clear @06:42:21.81
Carousel.in :default @06:42:21.92 Carousel.home.seek 52 @06:42:22.28 Selecting Cartridge 51 @06:42:22.55 Carousel.seek 51 @06:42:23.07 Lysis1.in :fast @06:42:23.12 Lysis1.seek :locked @06:42:25.74 Plunge.seek :locked @06:42:25.89 Air.in :fast @06:42:25.94 Air.seek :locked @06:42:26.91
Carousel.reconfigure :locked @06:42:27.05 Powering cartridge bus @06:42:29.70 Gate.setIR 1750,5000,0,6 @06:42:29.82 Gate.setIR 0,5000,0,6 @06:42:29.88 PuckHeater.configure :default @06:42:29.94 Gate.power :heat,:ON,limit to 5000mA @06:42:30.56 Mounted lysis Cartridge @06:42:30.59 WARNING: Using default puck temperature sensor calibration @06:42:30.60 Using default cartridge heat loss curve for HeatingAccelInCarousel @06:42:32.09 SPR.command "read_sequences" @06:42:32.49 SPR.command "stats_on" @06:42:32.63 SPR.command "start 0 1" @06:42:33.35 SPR.start /var/log/methane/spr24mar0706h.spr.bz2 @06:42:35.86 Waiting for baseline SPR stabilization @06:42:35.88 Handoff.select :waste @06:42:42.57 Syringe tare is 35% of full scale @06:42:42.60 PinPort.in :locking @06:42:42.65 PinPort.seek :locked @06:42:43.13 Archive.in :fast @06:42:43.20 Lysis2.in :fast @06:42:43.30 Lysis1.seek 1000uL @06:42:43.37 Archive.seek :full @06:42:43.45 Lysis2.seek 1000uL @06:42:48.06 delayUntil "06:52:35.83" @06:52:35.84 Lysis2.in :DAmix @06:52:35.95 Lysis1.in :DAmix @06:52:36.43 Lysis2.seek 750uL @06:52:36.53 Lysis1.seek 750uL @06:53:11.61 delay 5 seconds @06:53:16.63 Handoff.select SPR @06:53:16.75 SPR.command "start 2 14" @06:53:17.56 SPR stabilized @06:53:17.71 delay 10 seconds @06:53:27.73 SPR.command "start 1 0" @06:53:31.45 Waiting up to 4:00 for SPR to enter state 1 @06:55:36.16 SPR.command "hold_on" @06:55:37.55 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @06:55:38.15 Lysis2.seek 450uL @06:55:38.24 Lysis1.seek 450uL @06:56:20.31 delay 15 seconds @06:56:35.33 SPR.command "hold_off" @06:56:38.22 Waiting up to 1:00:00 for SPR to enter state 7 @07:40:43.17 SPRsummary:-12.3RIU,73.5RIU,118RIU,148RIU after 3687s @07:47:20.53 delay 10 seconds @07:47:30.56 SPR.command "start 1 0" @07:47:33.25 Waiting up to 4:00 for SPR to enter state 1 @07:49:37.53 SPR.command "hold_on" @07:49:38.92 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @07:49:39.55 Lysis2.seek 300uL @07:49:39.60 Lysis1.seek 300uL @07:50:00.70 delay 15 seconds @07:50:15.72 SPR.command "hold_off" @07:50:18.77 Waiting up to 1:00:00 for SPR to enter state 7 @08:41:01.09 delay 10 seconds @08:41:11.11 SPR.command "start 1 0" @08:41:13.81 Waiting up to 4:00 for SPR to enter state 1 @08:43:18.11 SPR.command "hold_on" @08:43:19.49 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @08:43:20.14 Lysis2.seek 150uL @08:43:20.26 Lysis1.seek 150uL @08:43:41.30 delay 15 seconds @08:43:56.32 SPR.command "hold_off" @08:43:59.33 Waiting up to 1:00:00 for SPR to enter state 7 @09:34:41.53 SPR analysis completed @09:34:49.34 Ended log spr24mar0706h.spr.bz2 @09:34:49.36 SPR.command "stats_off" @09:34:49.68 Handoff.select :close @09:34:49.83
Gate.power :heat,:OFF @09:34:49.84 Gate.power :cartridge,:OFF @09:34:50.39 PinPort.in :unlocking @09:34:50.45 PinPort.seek :clear @09:34:50.91 Carousel.in :default @09:34:51.02 Carousel.home.seek 51 @09:34:51.34 Plunge.seek :locked @09:34:51.50 Archive.in :unlocking @09:34:51.59 Archive.seek :clear @09:34:51.74 Air.in :unlocking @09:34:51.87 Air.seek :clear @09:34:51.92 Lysis1.in :unlocking @09:34:52.06 Lysis1.seek :clear @09:34:52.24 Lysis2.in :unlocking @09:34:52.29 Lysis2.seek :clear @09:34:56.59
Gate.power :motor,:OFF @09:34:56.60 Gate.power :core,:OFF @09:34:56.61 Gate.power :analytic1,:OFF @09:34:56.63 Gate.power :analytic2,:OFF @09:34:56.64 Gate.power :main,:OFF @09:34:56.65 Safely stopped and ready to power off @09:34:56.85 Gate.power :sampler,:OFF,limit to 3500mA @09:35:00.42 Gate.power :main,:ON,limit to 5000mA @09:35:00.44 Gate.power :analytic1,:ON,limit to 5000mA @09:35:00.45 Gate.power :analytic2,:ON,limit to 5000mA @09:35:00.55 Powering core bus @09:35:02.88 SPR Module 1, FW Rev. B, FPGA Rev. C @09:35:03.32
Plunge.configure :default @09:35:03.40 SamplePump.configure :default @09:35:03.47 Toroid.configure :default @09:35:03.55 Carousel.configure :default @09:35:03.62 Pressure.configure :default @09:35:03.70 ExtraPump.configure :default @09:35:03.77 Archive.configure :default @09:35:03.85 Air.configure :default @09:35:03.90 Lysis1.configure :default @09:35:03.95 Lysis2.configure :default @09:35:04.00 PinPort.configure :default @09:35:04.05 All elf controllers running firmware version 4.91 @09:35:04.07 Gate.power :motor,:ON,limit to 5000mA @09:35:04.40 Plunge.home.jog 0 @09:35:05.02 Lysis2.home.jog 0 @09:35:05.07 Lysis1.home.jog 0 @09:35:05.10 Archive.home.jog 0 @09:35:05.14 Carousel.home.jog 0 @09:35:05.17 Air.home.jog 0 @09:35:05.20 PinPort.home.jog 0 @09:35:05.23 Toroid.home.jog 0 @09:35:06.50 Plunge.in :homing @09:35:06.87 Plunge.jog 1553 @09:35:06.95 Lysis2.in :homing @09:35:06.98 Air.in :homing @09:35:07.00 Toroid.in :homing @09:35:07.02 PinPort.in :homing @09:35:07.04 Archive.in :homing @09:35:07.07 Lysis1.in :homing @09:35:07.32 Lysis2.jog 1394 @09:35:07.38 Air.jog 1358 @09:35:07.54 Lysis1.jog 1384 @09:35:07.56 Archive.jog 1386 @09:35:07.61 Toroid.jog 619 @09:35:07.64 PinPort.jog -102 @09:35:08.09 PinPort.setRawPosition! 0 @09:35:08.16 PinPort.seek :home @09:35:08.30 PinPort.in :default @09:35:08.39 Plunge.setRawPosition! 50 @09:35:08.44 Plunge.seek :home @09:35:08.65 Lysis2.setRawPosition! 50 @09:35:08.72 Plunge.in :default @09:35:08.76 Lysis2.seek :home @09:35:08.91 Air.setRawPosition! 50 @09:35:08.99 Archive.setRawPosition! 50 @09:35:09.02 Lysis1.setRawPosition! 50 @09:35:09.04 Air.seek :home @09:35:09.18 Lysis1.seek :home @09:35:09.26 Archive.seek :home @09:35:09.41 Lysis2.in :default @09:35:09.50 Air.in :default @09:35:09.60 Archive.in :default @09:35:09.62 Lysis1.in :default @09:35:09.94 Toroid.setRawPosition! 0 @09:35:09.99 Toroid.seek :home @09:35:10.08 Toroid.in :default @09:35:10.19 Toroid.seek :clear @09:35:11.86
Carousel.in :default @09:35:11.97 Carousel.home.seek 51 @09:35:12.30 Selecting Cartridge 50 @09:35:12.57 Carousel.seek 50 @09:35:13.08 Lysis1.in :fast @09:35:13.14 Lysis1.seek :locked @09:35:15.76 Plunge.seek :locked @09:35:15.91 Air.in :fast @09:35:15.96 Air.seek :locked @09:35:17.35
Carousel.reconfigure :locked @09:35:17.50 Powering cartridge bus @09:35:20.15 Gate.setIR 1750,5000,0,6 @09:35:20.28 Gate.setIR 0,5000,0,6 @09:35:20.35 PuckHeater.configure :default @09:35:20.41 Gate.power :heat,:ON,limit to 5000mA @09:35:21.03 Mounted lysis Cartridge @09:35:21.05 WARNING: Using default puck temperature sensor calibration @09:35:21.07 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:35:22.57 SPR.command "read_sequences" @09:35:22.63 SPR.command "stats_on" @09:35:22.78 SPR.command "start 0 1" @09:35:23.50 SPR.start /var/log/methane/spr24mar0709h.spr.bz2 @09:35:25.48 Waiting for baseline SPR stabilization @09:35:25.50 Handoff.select :waste @09:35:32.22 Syringe tare is 35% of full scale @09:35:32.25 PinPort.in :locking @09:35:32.30 PinPort.seek :locked @09:35:32.78 Archive.in :fast @09:35:32.86 Lysis2.in :fast @09:35:32.96 Archive.seek :full @09:35:33.00 Lysis1.seek 1000uL @09:35:33.04 Lysis2.seek 1000uL @09:35:37.63 delayUntil "09:45:25.04" @09:45:25.06 Lysis2.in :DAmix @09:45:25.17 Lysis1.in :DAmix @09:45:25.70 Lysis2.seek 750uL @09:45:25.76 Lysis1.seek 750uL @09:46:00.90 delay 5 seconds @09:46:05.92 Handoff.select SPR @09:46:06.04 SPR.command "start 2 14" @09:46:06.75 SPR stabilized @09:46:06.89 delay 10 seconds @09:46:16.91 SPR.command "start 1 0" @09:46:20.13 Waiting up to 4:00 for SPR to enter state 1 @09:48:24.41 SPR.command "hold_on" @09:48:26.32 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @09:48:26.90 Lysis2.seek 450uL @09:48:26.98 Lysis1.seek 450uL @09:49:09.00 delay 15 seconds @09:49:24.02 SPR.command "hold_off" @09:49:27.39 Waiting up to 1:00:00 for SPR to enter state 7 @10:33:31.42 SPRsummary:-16.5RIU,39.9RIU,79.5RIU,100RIU after 3685s @10:40:08.77 delay 10 seconds @10:40:18.80 SPR.command "start 1 0" @10:40:21.49 Waiting up to 4:00 for SPR to enter state 1 @10:42:25.79 SPR.command "hold_on" @10:42:27.17 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @10:42:27.77 Lysis2.seek 300uL @10:42:27.84 Lysis1.seek 300uL @10:42:48.91 delay 15 seconds @10:43:03.93 SPR.command "hold_off" @10:43:07.01 Waiting up to 1:00:00 for SPR to enter state 7 @11:33:49.34 delay 10 seconds @11:33:59.36 SPR.command "start 1 0" @11:34:02.05 Waiting up to 4:00 for SPR to enter state 1 @11:36:06.35 SPR.command "hold_on" @11:36:07.73 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @11:36:08.41 Lysis2.seek 150uL @11:36:08.47 Lysis1.seek 150uL @11:36:29.57 delay 15 seconds @11:36:44.59 SPR.command "hold_off" @11:36:47.58 Waiting up to 1:00:00 for SPR to enter state 7 @12:27:29.77 SPR analysis completed @12:27:37.58 Ended log spr24mar0709h.spr.bz2 @12:27:37.60 SPR.command "stats_off" @12:27:37.93 Handoff.select :close @12:27:38.08
Gate.power :heat,:OFF @12:27:38.10 Gate.power :cartridge,:OFF @12:27:38.20 PinPort.in :unlocking @12:27:38.26 PinPort.seek :clear @12:27:38.73 Carousel.in :default @12:27:38.84 Carousel.home.seek 50 @12:27:39.15 Plunge.seek :locked @12:27:39.31 Archive.in :unlocking @12:27:39.86 Archive.seek :clear @12:27:39.98 Air.in :unlocking @12:27:40.10 Air.seek :clear @12:27:40.24 Lysis1.in :unlocking @12:27:40.37 Lysis1.seek :clear @12:27:40.43 Lysis2.in :unlocking @12:27:40.53 Lysis2.seek :clear @12:27:44.82
Gate.power :motor,:OFF @12:27:44.84 Gate.power :core,:OFF @12:27:44.85 Gate.power :analytic1,:OFF @12:27:44.86 Gate.power :analytic2,:OFF @12:27:44.87 Gate.power :main,:OFF @12:27:44.89 Safely stopped and ready to power off @12:27:45.09 Gate.power :sampler,:OFF,limit to 3500mA @12:27:48.66 Gate.power :main,:ON,limit to 5000mA @12:27:48.68 Gate.power :analytic1,:ON,limit to 5000mA @12:27:48.69 Gate.power :analytic2,:ON,limit to 5000mA @12:27:48.79 Powering core bus @12:27:51.13 SPR Module 1, FW Rev. B, FPGA Rev. C @12:27:51.57
Plunge.configure :default @12:27:51.64 SamplePump.configure :default @12:27:51.72 Toroid.configure :default @12:27:51.80 Carousel.configure :default @12:27:51.87 Pressure.configure :default @12:27:51.95 ExtraPump.configure :default @12:27:52.02 Archive.configure :default @12:27:52.10 Air.configure :default @12:27:52.16 Lysis1.configure :default @12:27:52.23 Lysis2.configure :default @12:27:52.28 PinPort.configure :default @12:27:52.34 All elf controllers running firmware version 4.91 @12:27:52.36 Gate.power :motor,:ON,limit to 5000mA @12:27:52.87 Plunge.home.jog 0 @12:27:53.21 Lysis2.home.jog 0 @12:27:53.28 Lysis1.home.jog 0 @12:27:53.31 Archive.home.jog 0 @12:27:53.35 Toroid.home.jog 0 @12:27:53.38 PinPort.home.jog 0 @12:27:53.41 Carousel.home.jog 0 @12:27:53.46 Air.home.jog 0 @12:27:54.35 Plunge.in :homing @12:27:54.58 Plunge.jog 1553 @12:27:54.65 PinPort.in :homing @12:27:54.68 Lysis2.in :homing @12:27:54.70 Air.in :homing @12:27:54.72 Archive.in :homing @12:27:54.74 Toroid.in :homing @12:27:54.76 Lysis1.in :homing @12:27:55.05 PinPort.jog -104 @12:27:55.11 Lysis2.jog 1393 @12:27:55.64 Air.jog 1361 @12:27:55.67 Archive.jog 1387 @12:27:55.69 Lysis1.jog 1383 @12:27:55.72 Toroid.jog 618 @12:27:56.20 PinPort.setRawPosition! 0 @12:27:56.24 Plunge.setRawPosition! 50 @12:27:56.34 PinPort.seek :home @12:27:56.37 Plunge.seek :home @12:27:56.56 PinPort.in :default @12:27:56.71 Plunge.in :default @12:27:56.99 Lysis2.setRawPosition! 50 @12:27:57.02 Air.setRawPosition! 50 @12:27:57.16 Lysis2.seek :home @12:27:57.30 Air.seek :home @12:27:57.45 Archive.setRawPosition! 50 @12:27:57.47 Lysis1.setRawPosition! 50 @12:27:57.56 Archive.seek :home @12:27:57.61 Lysis1.seek :home @12:27:57.78 Lysis2.in :default @12:27:57.81 Air.in :default @12:27:57.95 Archive.in :default @12:27:58.03 Lysis1.in :default @12:27:58.10 Toroid.setRawPosition! 0 @12:27:58.17 Toroid.seek :home @12:27:58.27 Toroid.in :default @12:27:58.39 Toroid.seek :clear @12:28:00.10
Carousel.in :default @12:28:00.24 Carousel.home.seek 50 @12:28:00.56 Selecting Cartridge 49 @12:28:00.83 Carousel.seek 49 @12:28:01.36 Lysis1.in :fast @12:28:01.41 Lysis1.seek :locked @12:28:04.02 Plunge.seek :locked @12:28:04.21 Air.in :fast @12:28:04.26 Air.seek :locked @12:28:05.23
Carousel.reconfigure :locked @12:28:05.37 Powering cartridge bus @12:28:08.04 Gate.setIR 1750,5000,0,6 @12:28:08.16 Gate.setIR 0,5000,0,6 @12:28:08.26 PuckHeater.configure :default @12:28:08.32 Gate.power :heat,:ON,limit to 5000mA @12:28:08.94 Mounted lysis Cartridge @12:28:08.96 WARNING: Using default puck temperature sensor calibration @12:28:08.98 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:28:11.01 SPR.command "read_sequences" @12:28:11.81 SPR.command "stats_on" @12:28:11.97 SPR.command "start 0 1" @12:28:12.68 SPR.start /var/log/methane/spr24mar0712h.spr.bz2 @12:28:14.67 Waiting for baseline SPR stabilization @12:28:14.68 Handoff.select :waste @12:28:21.37 Syringe tare is 35% of full scale @12:28:21.40 PinPort.in :locking @12:28:21.44 PinPort.seek :locked @12:28:21.92 Archive.in :fast @12:28:21.97 Lysis2.in :fast @12:28:22.05 Lysis1.seek 1000uL @12:28:22.20 Archive.seek :full @12:28:22.35 Lysis2.seek 1000uL @12:28:26.89 delayUntil "12:38:14.24" @12:38:14.26 Lysis2.in :DAmix @12:38:14.37 Lysis1.in :DAmix @12:38:14.88 Lysis2.seek 750uL @12:38:14.94 Lysis1.seek 750uL @12:38:50.03 delay 5 seconds @12:38:55.05 Handoff.select SPR @12:38:55.18 SPR.command "start 2 14" @12:38:56.46 SPR stabilized @12:38:56.61 delay 10 seconds @12:39:06.63 SPR.command "start 1 0" @12:39:09.31 Waiting up to 4:00 for SPR to enter state 1 @12:41:13.60 SPR.command "hold_on" @12:41:14.99 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @12:41:15.61 Lysis2.seek 450uL @12:41:15.68 Lysis1.seek 450uL @12:41:57.71 delay 15 seconds @12:42:12.74 SPR.command "hold_off" @12:42:16.16 Waiting up to 1:00:00 for SPR to enter state 7 @13:26:21.55 SPRsummary:19.1RIU,44.2RIU,31.7RIU,35.1RIU after 3686s @13:32:58.90 delay 10 seconds @13:33:08.92 SPR.command "start 1 0" @13:33:11.62 Waiting up to 4:00 for SPR to enter state 1 @13:35:15.91 SPR.command "hold_on" @13:35:17.30 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @13:35:17.90 Lysis2.seek 300uL @13:35:17.96 Lysis1.seek 300uL @13:35:39.06 delay 15 seconds @13:35:54.08 SPR.command "hold_off" @13:35:57.15 Waiting up to 1:00:00 for SPR to enter state 7 @14:26:39.47 delay 10 seconds @14:26:49.49 SPR.command "start 1 0" @14:26:52.22 Waiting up to 4:00 for SPR to enter state 1 @14:28:56.49 SPR.command "hold_on" @14:28:58.39 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @14:28:59.01 Lysis2.seek 150uL @14:28:59.09 Lysis1.seek 150uL @14:29:20.16 delay 15 seconds @14:29:35.19 SPR.command "hold_off" @14:29:38.25 Waiting up to 1:00:00 for SPR to enter state 7 @15:20:19.90 SPR analysis completed @15:20:27.66 Ended log spr24mar0712h.spr.bz2 @15:20:27.67 SPR.command "stats_off" @15:20:28.07 Handoff.select :close @15:20:28.22
Gate.power :heat,:OFF @15:20:28.23 Gate.power :cartridge,:OFF @15:20:28.31 PinPort.in :unlocking @15:20:28.36 PinPort.seek :clear @15:20:28.82 Carousel.in :default @15:20:28.93 Carousel.home.seek 49 @15:20:29.24 Plunge.seek :locked @15:20:29.40 Archive.in :unlocking @15:20:29.51 Archive.seek :clear @15:20:29.61 Air.in :unlocking @15:20:30.22 Air.seek :clear @15:20:30.32 Lysis1.in :unlocking @15:20:30.44 Lysis1.seek :clear @15:20:30.57 Lysis2.in :unlocking @15:20:30.62 Lysis2.seek :clear @15:20:34.91
Gate.power :motor,:OFF @15:20:34.93 Gate.power :core,:OFF @15:20:34.94 Gate.power :analytic1,:OFF @15:20:34.95 Gate.power :analytic2,:OFF @15:20:34.97 Gate.power :main,:OFF @15:20:34.98 Safely stopped and ready to power off @15:20:35.18 Gate.power :sampler,:OFF,limit to 3500mA @15:20:38.75 Gate.power :main,:ON,limit to 5000mA @15:20:38.77 Gate.power :analytic1,:ON,limit to 5000mA @15:20:38.78 Gate.power :analytic2,:ON,limit to 5000mA @15:20:38.88 Powering core bus @15:20:41.21 SPR Module 1, FW Rev. B, FPGA Rev. C @15:20:41.65
Plunge.configure :default @15:20:41.72 SamplePump.configure :default @15:20:41.80 Toroid.configure :default @15:20:41.88 Carousel.configure :default @15:20:41.95 Pressure.configure :default @15:20:42.03 ExtraPump.configure :default @15:20:42.10 Archive.configure :default @15:20:42.20 Air.configure :default @15:20:42.26 Lysis1.configure :default @15:20:42.31 Lysis2.configure :default @15:20:42.36 PinPort.configure :default @15:20:42.41 All elf controllers running firmware version 4.91 @15:20:42.43 Gate.power :motor,:ON,limit to 5000mA @15:20:42.68 Plunge.home.jog 0 @15:20:43.34 PinPort.home.jog 0 @15:20:43.36 Lysis1.home.jog 0 @15:20:43.43 Air.home.jog 0 @15:20:43.46 Carousel.home.jog 0 @15:20:43.49 Toroid.home.jog 0 @15:20:43.52 Lysis2.home.jog 0 @15:20:43.56 Archive.home.jog 0 @15:20:44.43 Plunge.in :homing @15:20:44.66 Plunge.jog 1558 @15:20:44.78 PinPort.in :homing @15:20:44.89 Lysis1.in :homing @15:20:44.92 Air.in :homing @15:20:44.95 Archive.in :homing @15:20:44.97 Toroid.in :homing @15:20:45.00 Lysis2.in :homing @15:20:45.21 PinPort.jog -104 @15:20:45.29 Lysis1.jog 1385 @15:20:45.33 Air.jog 1363 @15:20:45.36 Archive.jog 1387 @15:20:45.39 Lysis2.jog 1393 @15:20:45.44 Toroid.jog 615 @15:20:46.38 PinPort.setRawPosition! 0 @15:20:46.42 Plunge.setRawPosition! 50 @15:20:46.53 PinPort.seek :home @15:20:46.56 Plunge.seek :home @15:20:46.77 PinPort.in :default @15:20:46.79 Lysis1.setRawPosition! 50 @15:20:46.90 Lysis1.seek :home @15:20:47.01 Plunge.in :default @15:20:47.20 Lysis1.in :default @15:20:47.22 Archive.setRawPosition! 50 @15:20:47.33 Archive.seek :home @15:20:47.41 Air.setRawPosition! 50 @15:20:47.45 Lysis2.setRawPosition! 50 @15:20:47.55 Lysis2.seek :home @15:20:47.58 Air.seek :home @15:20:47.74 Archive.in :default @15:20:47.88 Lysis2.in :default @15:20:47.97 Air.in :default @15:20:48.25 Toroid.setRawPosition! 0 @15:20:48.30 Toroid.seek :home @15:20:48.38 Toroid.in :default @15:20:48.46 Toroid.seek :clear @15:20:50.14
Carousel.in :default @15:20:50.29 Carousel.home.seek 49 @15:20:50.55 Selecting Cartridge 48 @15:20:50.82 Carousel.seek 48 @15:20:51.34 Lysis1.in :fast @15:20:51.41 Lysis1.seek :locked @15:20:54.02 Plunge.seek :locked @15:20:54.18 Air.in :fast @15:20:54.25 Air.seek :locked @15:20:55.22
Carousel.reconfigure :locked @15:20:55.37 Powering cartridge bus @15:20:58.04 Gate.setIR 1750,5000,0,6 @15:20:58.18 Gate.setIR 0,5000,0,6 @15:20:58.26 PuckHeater.configure :default @15:20:58.32 Gate.power :heat,:ON,limit to 5000mA @15:20:58.93 Mounted lysis Cartridge @15:20:58.96 WARNING: Using default puck temperature sensor calibration @15:20:58.97 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:21:00.51 SPR.command "read_sequences" @15:21:01.13 SPR.command "stats_on" @15:21:01.99 SPR.command "start 0 1" @15:21:02.71 SPR.start /var/log/methane/spr24mar0715h.spr.bz2 @15:21:05.28 Waiting for baseline SPR stabilization @15:21:05.29 Handoff.select :waste @15:21:11.98 Syringe tare is 35% of full scale @15:21:12.00 PinPort.in :locking @15:21:12.06 PinPort.seek :locked @15:21:12.55 Archive.in :fast @15:21:12.63 Lysis2.in :fast @15:21:12.69 Lysis1.seek 1000uL @15:21:12.71 Archive.seek :full @15:21:12.86 Lysis2.seek 1000uL @15:21:17.45 delayUntil "15:31:05.24" @15:31:05.26 Lysis2.in :DAmix @15:31:05.37 Lysis1.in :DAmix @15:31:05.89 Lysis2.seek 750uL @15:31:05.94 Lysis1.seek 750uL @15:31:41.06 delay 5 seconds @15:31:46.09 Handoff.select SPR @15:31:46.21 SPR.command "start 2 14" @15:31:46.97 SPR stabilized @15:31:47.11 delay 10 seconds @15:31:57.13 SPR.command "start 1 0" @15:32:00.34 Waiting up to 4:00 for SPR to enter state 1 @15:34:04.63 SPR.command "hold_on" @15:34:06.02 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @15:34:06.64 Lysis2.seek 450uL @15:34:06.70 Lysis1.seek 450uL @15:34:48.75 delay 15 seconds @15:35:03.77 SPR.command "hold_off" @15:35:06.67 Waiting up to 1:00:00 for SPR to enter state 7 @16:19:11.64 SPRsummary:-86.2RIU,-26.5RIU,39.7RIU,44.2RIU after 3686s @16:25:48.99 delay 10 seconds @16:25:59.01 SPR.command "start 1 0" @16:26:01.71 Waiting up to 4:00 for SPR to enter state 1 @16:28:06.01 SPR.command "hold_on" @16:28:07.39 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @16:28:08.10 Lysis2.seek 300uL @16:28:08.16 Lysis1.seek 300uL @16:28:29.29 delay 15 seconds @16:28:44.31 SPR.command "hold_off" @16:28:47.75 Waiting up to 1:00:00 for SPR to enter state 7 @17:19:30.50 delay 10 seconds @17:19:40.52 SPR.command "start 1 0" @17:19:43.23 Waiting up to 4:00 for SPR to enter state 1 @17:21:22.38 Session Begun @17:21:24.06 -> showlog @17:21:40.53 -> showlog -100 @17:21:47.52 SPR.command "hold_on" @17:21:48.90 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @17:21:49.50 Lysis2.seek 150uL @17:21:49.57 Lysis1.seek 150uL @17:22:10.69 delay 15 seconds @17:22:25.72 SPR.command "hold_off" @17:22:28.74 Waiting up to 1:00:00 for SPR to enter state 7 @17:24:04.31 Session Begun @17:24:10.90 -> cd /var/log @17:24:10.93 SyntaxError in bill2 -- compile error bill2:1: unknown regexp options - lg @17:24:19.70 -> exit @17:24:19.73 Session Ended @17:48:20.78 Session Ended @18:03:00.83 Session Begun @18:03:03.68 -> showlog @18:03:09.95 -> showlog -50 @18:13:10.94 SPR analysis completed @18:13:18.74 Ended log spr24mar0715h.spr.bz2 @18:13:18.77 SPR.command "stats_off" @18:13:19.11 Handoff.select :close @18:13:19.28
Gate.power :heat,:OFF @18:13:19.30 Gate.power :cartridge,:OFF @18:13:19.43 PinPort.in :unlocking @18:13:19.51 PinPort.seek :clear @18:13:20.02 Carousel.in :default @18:13:20.16 Carousel.home.seek 48 @18:13:20.55 Plunge.seek :locked @18:13:20.75 Archive.in :unlocking @18:13:20.89 Archive.seek :clear @18:13:21.12 Air.in :unlocking @18:13:21.28 Air.seek :clear @18:13:21.84 Lysis1.in :unlocking @18:13:22.05 Lysis1.seek :clear @18:13:22.24 Lysis2.in :unlocking @18:13:22.32 Lysis2.seek :clear @18:13:26.64
Gate.power :motor,:OFF @18:13:26.66 Gate.power :core,:OFF @18:13:26.70 Gate.power :analytic1,:OFF @18:13:26.73 Gate.power :analytic2,:OFF @18:13:26.77 Gate.power :main,:OFF @18:13:26.80 Safely stopped and ready to power off @18:13:27.33 Gate.power :sampler,:OFF,limit to 3500mA @18:13:30.51 Gate.power :main,:ON,limit to 5000mA @18:13:30.53 Gate.power :analytic1,:ON,limit to 5000mA @18:13:30.56 Gate.power :analytic2,:ON,limit to 5000mA @18:13:30.70 Powering core bus @18:13:33.02 SPR Module 1, FW Rev. B, FPGA Rev. C @18:13:33.48
Plunge.configure :default @18:13:33.57 SamplePump.configure :default @18:13:33.67 Toroid.configure :default @18:13:33.78 Carousel.configure :default @18:13:33.88 Pressure.configure :default @18:13:33.99 ExtraPump.configure :default @18:13:34.08 Archive.configure :default @18:13:34.20 Air.configure :default @18:13:34.29 Lysis1.configure :default @18:13:34.38 Lysis2.configure :default @18:13:34.45 PinPort.configure :default @18:13:34.53 All elf controllers running firmware version 4.91 @18:13:34.55 Gate.power :motor,:ON,limit to 5000mA @18:13:34.91 Toroid.home.jog 0 @18:13:35.56 PinPort.home.jog 0 @18:13:35.62 Lysis1.home.jog 0 @18:13:35.66 Archive.home.jog 0 @18:13:35.70 Lysis2.home.jog 0 @18:13:35.77 Carousel.home.jog 0 @18:13:35.81 Plunge.home.jog 0 @18:13:35.86 Air.home.jog 0 @18:13:36.65 Toroid.in :homing @18:13:36.89 Toroid.jog 612 @18:13:37.10 Lysis2.in :homing @18:13:37.16 Lysis1.in :homing @18:13:37.21 Archive.in :homing @18:13:37.25 PinPort.in :homing @18:13:37.30 Plunge.in :homing @18:13:37.35 Air.in :homing @18:13:37.63 Lysis2.jog 1393 @18:13:37.67 Archive.jog 1387 @18:13:37.77 Lysis1.jog 1388 @18:13:37.88 PinPort.jog -104 @18:13:37.99 Air.jog 1361 @18:13:38.06 Plunge.jog 1556 @18:13:38.90 PinPort.setRawPosition! 0 @18:13:39.14 PinPort.seek :home @18:13:39.17 Lysis2.setRawPosition! 50 @18:13:39.25 Toroid.setRawPosition! 0 @18:13:39.41 Lysis2.seek :home @18:13:39.53 Toroid.seek :home @18:13:39.76 Lysis1.setRawPosition! 50 @18:13:39.82 Air.setRawPosition! 50 @18:13:39.86 Toroid.in :default @18:13:39.91 PinPort.in :default @18:13:39.96 Plunge.setRawPosition! 50 @18:13:39.99 Archive.setRawPosition! 50 @18:13:40.31 Lysis1.seek :home @18:13:40.41 Air.seek :home @18:13:40.47 Archive.seek :home @18:13:40.55 Plunge.seek :home @18:13:40.85 Lysis1.in :default @18:13:40.89 Lysis2.in :default @18:13:41.10 Archive.in :default @18:13:41.14 Plunge.in :default @18:13:41.18 Air.in :default @18:13:41.63 Toroid.seek :clear @18:13:43.36
Carousel.in :default @18:13:43.51 Carousel.home.seek 48 @18:13:43.86 Selecting Cartridge 47 @18:13:44.16 Carousel.seek 47 @18:13:44.75 Lysis1.in :fast @18:13:44.81 Lysis1.seek :locked @18:13:47.46 Plunge.seek :locked @18:13:47.64 Air.in :fast @18:13:47.71 Air.seek :locked @18:13:48.71
Carousel.reconfigure :locked @18:13:48.89 Powering cartridge bus @18:13:51.54 Gate.setIR 1750,5000,0,6 @18:13:51.67 Gate.setIR 0,5000,0,6 @18:13:51.76 PuckHeater.configure :default @18:13:51.85 Gate.power :heat,:ON,limit to 5000mA @18:13:52.48 Mounted lysis Cartridge @18:13:52.51 WARNING: Using default puck temperature sensor calibration @18:13:52.55 Using default cartridge heat loss curve for HeatingAccelInCarousel @18:13:54.12 SPR.command "read_sequences" @18:13:54.62 SPR.command "stats_on" @18:13:55.26 SPR.command "start 0 1" @18:13:55.50 SPR.start /var/log/methane/spr24mar0718h.spr.bz2 @18:13:57.47 Waiting for baseline SPR stabilization @18:13:57.49 Handoff.select :waste @18:14:04.26 Syringe tare is 35% of full scale @18:14:04.29 PinPort.in :locking @18:14:04.39 PinPort.seek :locked @18:14:04.91 Archive.in :fast @18:14:05.02 Lysis2.in :fast @18:14:05.06 Lysis1.seek 1000uL @18:14:05.19 Archive.seek :full @18:14:05.27 Lysis2.seek 1000uL @18:14:09.90 delayUntil "18:23:57.04" @18:23:57.06 Lysis2.in :DAmix @18:23:57.20 Lysis1.in :DAmix @18:23:57.75 Lysis2.seek 750uL @18:23:57.80 Lysis1.seek 750uL @18:24:32.98 delay 5 seconds @18:24:38.01 Handoff.select SPR @18:24:38.17 SPR.command "start 2 14" @18:24:38.75 SPR stabilized @18:24:38.93 delay 10 seconds @18:24:48.95 SPR.command "start 1 0" @18:24:52.12 Waiting up to 4:00 for SPR to enter state 1 @18:26:56.42 SPR.command "hold_on" @18:26:57.79 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @18:26:58.50 Lysis2.seek 450uL @18:26:58.57 Lysis1.seek 450uL @18:27:40.65 delay 15 seconds @18:27:55.67 SPR.command "hold_off" @18:27:58.45 Waiting up to 1:00:00 for SPR to enter state 7 @19:05:50.94 -> showlog @19:12:03.42 SPRsummary:-10.7RIU,1.87RIU,24.1RIU,25RIU after 3685s @19:18:40.77 delay 10 seconds @19:18:50.79 SPR.command "start 1 0" @19:18:53.49 Waiting up to 4:00 for SPR to enter state 1 @19:20:57.79 SPR.command "hold_on" @19:20:59.17 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @19:20:59.79 Lysis2.seek 300uL @19:20:59.85 Lysis1.seek 300uL @19:21:21.00 delay 15 seconds @19:21:36.03 SPR.command "hold_off" @19:21:39.01 Waiting up to 1:00:00 for SPR to enter state 7 @19:49:14.51 Session Ended @20:12:21.34 delay 10 seconds @20:12:31.36 SPR.command "start 1 0" @20:12:34.06 Waiting up to 4:00 for SPR to enter state 1 @20:14:38.34 SPR.command "hold_on" @20:14:39.73 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @20:14:40.43 Lysis2.seek 150uL @20:14:40.46 Lysis1.seek 150uL @20:15:01.58 delay 15 seconds @20:15:16.62 SPR.command "hold_off" @20:15:19.58 Waiting up to 1:00:00 for SPR to enter state 7 @21:06:01.77 SPR analysis completed @21:06:09.65 Ended log spr24mar0718h.spr.bz2 @21:06:09.67 SPR.command "stats_off" @21:06:09.93 Handoff.select :close @21:06:10.09
Gate.power :heat,:OFF @21:06:10.10 Gate.power :cartridge,:OFF @21:06:10.19 PinPort.in :unlocking @21:06:10.26 PinPort.seek :clear @21:06:11.17 Carousel.in :default @21:06:11.28 Carousel.home.seek 47 @21:06:11.60 Plunge.seek :locked @21:06:11.75 Archive.in :unlocking @21:06:11.87 Archive.seek :clear @21:06:11.96 Air.in :unlocking @21:06:12.10 Air.seek :clear @21:06:12.23 Lysis1.in :unlocking @21:06:12.42 Lysis1.seek :clear @21:06:12.47 Lysis2.in :unlocking @21:06:12.58 Lysis2.seek :clear @21:06:16.87
Gate.power :motor,:OFF @21:06:16.88 Gate.power :core,:OFF @21:06:16.90 Gate.power :analytic1,:OFF @21:06:16.91 Gate.power :analytic2,:OFF @21:06:16.92 Gate.power :main,:OFF @21:06:16.93 Safely stopped and ready to power off @21:06:17.12 Gate.power :sampler,:OFF,limit to 3500mA @21:06:20.71 Gate.power :main,:ON,limit to 5000mA @21:06:20.72 Gate.power :analytic1,:ON,limit to 5000mA @21:06:20.74 Gate.power :analytic2,:ON,limit to 5000mA @21:06:20.83 Powering core bus @21:06:23.17 SPR Module 1, FW Rev. B, FPGA Rev. C @21:06:23.59
Plunge.configure :default @21:06:23.67 SamplePump.configure :default @21:06:23.75 Toroid.configure :default @21:06:23.83 Carousel.configure :default @21:06:23.90 Pressure.configure :default @21:06:23.98 ExtraPump.configure :default @21:06:24.06 Archive.configure :default @21:06:24.14 Air.configure :default @21:06:24.21 Lysis1.configure :default @21:06:24.27 Lysis2.configure :default @21:06:24.32 PinPort.configure :default @21:06:24.37 All elf controllers running firmware version 4.91 @21:06:24.39 Gate.power :motor,:ON,limit to 5000mA @21:06:24.58 Plunge.home.jog 0 @21:06:25.31 Lysis2.home.jog 0 @21:06:25.34 Air.home.jog 0 @21:06:25.39 Archive.home.jog 0 @21:06:25.42 Toroid.home.jog 0 @21:06:25.45 PinPort.home.jog 0 @21:06:25.48 Lysis1.home.jog 0 @21:06:25.50 Carousel.home.jog 0 @21:06:26.79 Plunge.in :homing @21:06:26.99 Plunge.jog 1554 @21:06:27.20 Lysis2.in :homing @21:06:27.26 Lysis1.in :homing @21:06:27.28 Archive.in :homing @21:06:27.31 PinPort.in :homing @21:06:27.33 Air.in :homing @21:06:27.36 Toroid.in :homing @21:06:27.53 Lysis1.jog 1385 @21:06:27.57 Lysis2.jog 1393 @21:06:27.71 Archive.jog 1387 @21:06:27.84 Air.jog 1364 @21:06:27.93 Toroid.jog 616 @21:06:27.95 PinPort.jog -103 @21:06:28.30 Plunge.setRawPosition! 50 @21:06:28.36 Plunge.seek :home @21:06:28.45 PinPort.setRawPosition! 0 @21:06:28.51 PinPort.seek :home @21:06:28.61 PinPort.in :default @21:06:28.69 Plunge.in :default @21:06:28.82 Lysis1.setRawPosition! 50 @21:06:28.90 Lysis2.setRawPosition! 50 @21:06:28.92 Lysis1.seek :home @21:06:29.05 Lysis2.seek :home @21:06:29.16 Archive.setRawPosition! 50 @21:06:29.22 Archive.seek :home @21:06:29.35 Lysis2.in :default @21:06:29.39 Lysis1.in :default @21:06:29.41 Air.setRawPosition! 50 @21:06:29.57 Archive.in :default @21:06:29.60 Air.seek :home @21:06:29.93 Air.in :default @21:06:30.24 Toroid.setRawPosition! 0 @21:06:30.30 Toroid.seek :home @21:06:30.38 Toroid.in :default @21:06:30.45 Toroid.seek :clear @21:06:32.12
Carousel.in :default @21:06:32.27 Carousel.home.seek 47 @21:06:32.59 Selecting Cartridge 46 @21:06:32.86 Carousel.seek 46 @21:06:33.39 Lysis1.in :fast @21:06:33.43 Lysis1.seek :locked @21:06:36.04 Plunge.seek :locked @21:06:36.23 Air.in :fast @21:06:36.28 Air.seek :locked @21:06:37.25
Carousel.reconfigure :locked @21:06:37.84 Powering cartridge bus @21:06:40.54 Gate.setIR 1750,5000,0,6 @21:06:40.66 Gate.setIR 0,5000,0,6 @21:06:40.73 PuckHeater.configure :default @21:06:40.78 Gate.power :heat,:ON,limit to 5000mA @21:06:41.40 Mounted lysis Cartridge @21:06:41.43 WARNING: Using default puck temperature sensor calibration @21:06:41.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:06:42.94 SPR.command "read_sequences" @21:06:43.00 SPR.command "stats_on" @21:06:43.16 SPR.command "start 0 1" @21:06:43.87 SPR.start /var/log/methane/spr24mar0721h.spr.bz2 @21:06:45.76 Waiting for baseline SPR stabilization @21:06:45.78 Handoff.select :waste @21:06:52.47 Syringe tare is 35% of full scale @21:06:52.50 PinPort.in :locking @21:06:52.55 PinPort.seek :locked @21:06:53.03 Archive.in :fast @21:06:53.11 Lysis2.in :fast @21:06:53.14 Lysis1.seek 1000uL @21:06:53.31 Lysis2.seek 1000uL @21:06:53.35 Archive.seek :full @21:06:57.88 delayUntil "21:16:45.33" @21:16:45.34 Lysis2.in :DAmix @21:16:45.45 Lysis1.in :DAmix @21:16:46.03 Lysis2.seek 750uL @21:16:46.07 Lysis1.seek 750uL @21:17:21.16 delay 5 seconds @21:17:26.19 Handoff.select SPR @21:17:26.34 SPR.command "start 2 14" @21:17:27.04 SPR stabilized @21:17:27.18 delay 10 seconds @21:17:37.20 SPR.command "start 1 0" @21:17:40.93 Waiting up to 4:00 for SPR to enter state 1 @21:19:45.65 SPR.command "hold_on" @21:19:47.03 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @21:19:47.65 Lysis2.seek 450uL @21:19:47.72 Lysis1.seek 450uL @21:20:29.78 delay 15 seconds @21:20:44.81 SPR.command "hold_off" @21:20:47.68 Waiting up to 1:00:00 for SPR to enter state 7 @22:04:52.65 SPRsummary:-11.1RIU,-3.26RIU,17.6RIU,18RIU after 3686s @22:11:30.02 delay 10 seconds @22:11:40.04 SPR.command "start 1 0" @22:11:42.73 Waiting up to 4:00 for SPR to enter state 1 @22:13:47.01 SPR.command "hold_on" @22:13:48.40 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @22:13:48.99 Lysis2.seek 300uL @22:13:49.06 Lysis1.seek 300uL @22:14:10.14 delay 15 seconds @22:14:25.16 SPR.command "hold_off" @22:14:28.25 Waiting up to 1:00:00 for SPR to enter state 7 @23:05:10.57 delay 10 seconds @23:05:20.59 SPR.command "start 1 0" @23:05:23.29 Waiting up to 4:00 for SPR to enter state 1 @23:07:27.57 SPR.command "hold_on" @23:07:28.96 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @23:07:29.64 Lysis2.seek 150uL @23:07:29.70 Lysis1.seek 150uL @23:07:50.77 delay 15 seconds @23:08:05.79 SPR.command "hold_off" @23:08:08.81 Waiting up to 1:00:00 for SPR to enter state 7 @23:58:51.00 SPR analysis completed @23:58:58.82 Ended log spr24mar0721h.spr.bz2 @23:58:58.84 SPR.command "stats_off" @23:58:59.20 Handoff.select :close @23:58:59.35
Gate.power :heat,:OFF @23:58:59.37 Gate.power :cartridge,:OFF @23:58:59.45 PinPort.in :unlocking @23:58:59.50 PinPort.seek :clear @23:58:59.97 Carousel.in :default @23:59:00.07 Carousel.home.seek 46 @23:59:00.40 Plunge.seek :locked @23:59:00.57 Archive.in :unlocking @23:59:00.70 Archive.seek :clear @23:59:00.80 Air.in :unlocking @23:59:01.40 Air.seek :clear @23:59:01.47 Lysis1.in :unlocking @23:59:01.65 Lysis1.seek :clear @23:59:01.71 Lysis2.in :unlocking @23:59:01.83 Lysis2.seek :clear @23:59:06.13
Gate.power :motor,:OFF @23:59:06.14 Gate.power :core,:OFF @23:59:06.15 Gate.power :analytic1,:OFF @23:59:06.16 Gate.power :analytic2,:OFF @23:59:06.19 Gate.power :main,:OFF @23:59:06.21 Safely stopped and ready to power off @23:59:06.73 Gate.power :sampler,:OFF,limit to 3500mA @23:59:09.96 Gate.power :main,:ON,limit to 5000mA @23:59:09.98 Gate.power :analytic1,:ON,limit to 5000mA @23:59:09.99 Gate.power :analytic2,:ON,limit to 5000mA @23:59:10.09 Powering core bus @23:59:12.42 SPR Module 1, FW Rev. B, FPGA Rev. C @23:59:12.86
Plunge.configure :default @23:59:12.94 SamplePump.configure :default @23:59:13.02 Toroid.configure :default @23:59:13.09 Carousel.configure :default @23:59:13.17 Pressure.configure :default @23:59:13.24 ExtraPump.configure :default @23:59:13.32 Archive.configure :default @23:59:13.39 Air.configure :default @23:59:13.45 Lysis1.configure :default @23:59:13.50 Lysis2.configure :default @23:59:13.55 PinPort.configure :default @23:59:13.60 All elf controllers running firmware version 4.91 @23:59:13.62 Gate.power :motor,:ON,limit to 5000mA @23:59:13.82 Plunge.home.jog 0 @23:59:14.54 Carousel.home.jog 0 @23:59:14.64 PinPort.home.jog 0 @23:59:14.68 Lysis2.home.jog 0 @23:59:14.71 Air.home.jog 0 @23:59:14.75 Archive.home.jog 0 @23:59:14.80 Lysis1.home.jog 0 @23:59:14.82 Toroid.home.jog 0 @23:59:15.54 Plunge.in :homing @23:59:15.91 Plunge.jog 1553 @23:59:15.99 Lysis2.in :homing @23:59:16.02 Air.in :homing @23:59:16.04 Toroid.in :homing @23:59:16.06 Archive.in :homing @23:59:16.09 Lysis1.in :homing @23:59:16.12 PinPort.in :homing @23:59:16.36 Lysis2.jog 1394 @23:59:16.52 PinPort.jog -103 @23:59:16.58 Lysis1.jog 1388 @23:59:16.60 Air.jog 1359 @23:59:16.64 Archive.jog 1387 @23:59:16.66 Toroid.jog 615 @23:59:17.60 PinPort.setRawPosition! 0 @23:59:17.64 Plunge.setRawPosition! 50 @23:59:17.74 PinPort.seek :home @23:59:17.77 Plunge.seek :home @23:59:17.95 Lysis2.setRawPosition! 50 @23:59:17.98 PinPort.in :default @23:59:18.08 Lysis2.seek :home @23:59:18.22 Plunge.in :default @23:59:18.37 Lysis2.in :default @23:59:18.49 Lysis1.setRawPosition! 50 @23:59:18.52 Air.setRawPosition! 50 @23:59:18.54 Archive.setRawPosition! 50 @23:59:18.68 Lysis1.seek :home @23:59:18.74 Archive.seek :home @23:59:18.77 Air.seek :home @23:59:19.11 Lysis1.in :default @23:59:19.21 Air.in :default @23:59:19.23 Archive.in :default @23:59:19.40 Toroid.setRawPosition! 0 @23:59:19.45 Toroid.seek :home @23:59:19.54 Toroid.in :default @23:59:19.64 Toroid.seek :clear @23:59:21.37
Carousel.in :default @23:59:21.48 Carousel.home.seek 46 @23:59:21.80 Selecting Cartridge 45 @23:59:22.08 Carousel.seek 45 @23:59:22.61 Lysis1.in :fast @23:59:22.68 Lysis1.seek :locked @23:59:25.30 Plunge.seek :locked @23:59:25.45 Air.in :fast @23:59:25.50 Air.seek :locked @23:59:26.47
Carousel.reconfigure :locked @23:59:26.61 Powering cartridge bus @23:59:29.26 Gate.setIR 1750,5000,0,6 @23:59:29.38 Gate.setIR 0,5000,0,6 @23:59:29.44 PuckHeater.configure :default @23:59:29.50 Gate.power :heat,:ON,limit to 5000mA @23:59:30.11 Mounted lysis Cartridge @23:59:30.14 WARNING: Using default puck temperature sensor calibration @23:59:30.15 Using default cartridge heat loss curve for HeatingAccelInCarousel @23:59:31.69 SPR.command "read_sequences" @23:59:32.35 SPR.command "stats_on" @23:59:33.21 SPR.command "start 0 1" @23:59:33.92 SPR.start /var/log/methane/spr24mar0723h.spr.bz2 @23:59:35.97 Waiting for baseline SPR stabilization @23:59:35.98 Handoff.select :waste @23:59:42.68 Syringe tare is 35% of full scale @23:59:42.71 PinPort.in :locking @23:59:42.76 PinPort.seek :locked @23:59:43.24 Archive.in :fast @23:59:43.33 Lysis2.in :fast @23:59:43.38 Lysis1.seek 1000uL @23:59:43.43 Archive.seek :full @23:59:43.51 Lysis2.seek 1000uL @23:59:48.06 delayUntil "00:09:35.53UTC08-Mar-24" @00:09:35.55UTC08-Mar-24 Lysis2.in :DAmix @00:09:35.66 Lysis1.in :DAmix @00:09:36.25 Lysis2.seek 750uL @00:09:36.29 Lysis1.seek 750uL @00:10:11.42 delay 5 seconds @00:10:16.44 Handoff.select SPR @00:10:16.57 SPR.command "start 2 14" @00:10:17.24 SPR stabilized @00:10:17.38 delay 10 seconds @00:10:27.40 SPR.command "start 1 0" @00:10:30.60 Waiting up to 4:00 for SPR to enter state 1 @00:12:34.90 SPR.command "hold_on" @00:12:36.28 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @00:12:36.90 Lysis2.seek 450uL @00:12:36.95 Lysis1.seek 450uL @00:13:19.01 delay 15 seconds @00:13:34.03 SPR.command "hold_off" @00:13:37.46 Waiting up to 1:00:00 for SPR to enter state 7 @00:57:42.85 SPRsummary:-13RIU,3.19RIU,26.6RIU,31.3RIU after 3686s @01:04:20.24 delay 10 seconds @01:04:30.26 SPR.command "start 1 0" @01:04:32.92 Waiting up to 4:00 for SPR to enter state 1 @01:06:37.21 SPR.command "hold_on" @01:06:39.12 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @01:06:39.74 Lysis2.seek 300uL @01:06:39.79 Lysis1.seek 300uL @01:07:00.90 delay 15 seconds @01:07:15.92 SPR.command "hold_off" @01:07:18.44 Waiting up to 1:00:00 for SPR to enter state 7 @01:57:59.83 delay 10 seconds @01:58:09.85 SPR.command "start 1 0" @01:58:12.54 Waiting up to 4:00 for SPR to enter state 1 @02:00:16.83 SPR.command "hold_on" @02:00:18.24 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @02:00:18.84 Lysis2.seek 150uL @02:00:18.88 Lysis1.seek 150uL @02:00:39.99 delay 15 seconds @02:00:55.01 SPR.command "hold_off" @02:00:58.06 Waiting up to 1:00:00 for SPR to enter state 7 @02:51:40.26 SPR analysis completed @02:51:48.52 Ended log spr24mar0723h.spr.bz2 @02:51:48.54 SPR.command "stats_off" @02:51:49.36 Handoff.select :close @02:51:49.51
Gate.power :heat,:OFF @02:51:49.53 Gate.power :cartridge,:OFF @02:51:50.06 PinPort.in :unlocking @02:51:50.11 PinPort.seek :clear @02:51:50.57 Carousel.in :default @02:51:50.68 Carousel.home.seek 45 @02:51:51.00 Plunge.seek :locked @02:51:51.16 Archive.in :unlocking @02:51:51.27 Archive.seek :clear @02:51:51.38 Air.in :unlocking @02:51:51.51 Air.seek :clear @02:51:51.59 Lysis1.in :unlocking @02:51:51.73 Lysis1.seek :clear @02:51:51.84 Lysis2.in :unlocking @02:51:51.91 Lysis2.seek :clear @02:51:56.23
Gate.power :motor,:OFF @02:51:56.25 Gate.power :core,:OFF @02:51:56.26 Gate.power :analytic1,:OFF @02:51:56.27 Gate.power :analytic2,:OFF @02:51:56.29 Gate.power :main,:OFF @02:51:56.30 Safely stopped and ready to power off @02:51:56.75 Gate.power :sampler,:OFF,limit to 3500mA @02:52:00.07 Gate.power :main,:ON,limit to 5000mA @02:52:00.09 Gate.power :analytic1,:ON,limit to 5000mA @02:52:00.10 Gate.power :analytic2,:ON,limit to 5000mA @02:52:00.22 Powering core bus @02:52:02.53 SPR Module 1, FW Rev. B, FPGA Rev. C @02:52:03.00
Plunge.configure :default @02:52:03.07 SamplePump.configure :default @02:52:03.15 Toroid.configure :default @02:52:03.23 Carousel.configure :default @02:52:03.30 Pressure.configure :default @02:52:03.38 ExtraPump.configure :default @02:52:03.45 Archive.configure :default @02:52:03.53 Air.configure :default @02:52:03.58 Lysis1.configure :default @02:52:03.63 Lysis2.configure :default @02:52:03.68 PinPort.configure :default @02:52:03.73 All elf controllers running firmware version 4.91 @02:52:03.75 Gate.power :motor,:ON,limit to 5000mA @02:52:03.95 Plunge.home.jog 0 @02:52:04.68 PinPort.home.jog 0 @02:52:04.74 Lysis1.home.jog 0 @02:52:04.76 Air.home.jog 0 @02:52:04.79 Archive.home.jog 0 @02:52:04.82 Carousel.home.jog 0 @02:52:04.86 Toroid.home.jog 0 @02:52:04.89 Lysis2.home.jog 0 @02:52:06.20 Plunge.in :homing @02:52:06.55 Plunge.jog 1551 @02:52:06.63 Lysis2.in :homing @02:52:06.67 Air.in :homing @02:52:06.69 Toroid.in :homing @02:52:06.71 PinPort.in :homing @02:52:06.73 Archive.in :homing @02:52:06.74 Lysis1.in :homing @02:52:07.04 Lysis2.jog 1392 @02:52:07.11 PinPort.jog -105 @02:52:07.13 Air.jog 1360 @02:52:07.15 Toroid.jog 611 @02:52:07.19 Lysis1.jog 1385 @02:52:07.26 Archive.jog 1387 @02:52:07.62 PinPort.setRawPosition! 0 @02:52:07.68 PinPort.seek :home @02:52:07.76 PinPort.in :default @02:52:08.04 Plunge.setRawPosition! 50 @02:52:08.09 Plunge.seek :home @02:52:08.37 Plunge.in :default @02:52:08.44 Lysis2.setRawPosition! 50 @02:52:08.53 Air.setRawPosition! 50 @02:52:08.56 Lysis2.seek :home @02:52:08.67 Air.seek :home @02:52:08.77 Archive.setRawPosition! 50 @02:52:08.79 Lysis1.setRawPosition! 50 @02:52:08.88 Lysis1.seek :home @02:52:08.94 Archive.seek :home @02:52:09.11 Air.in :default @02:52:09.13 Lysis2.in :default @02:52:09.29 Archive.in :default @02:52:09.31 Lysis1.in :default @02:52:09.46 Toroid.setRawPosition! 0 @02:52:09.52 Toroid.seek :home @02:52:09.60 Toroid.in :default @02:52:09.71 Toroid.seek :clear @02:52:11.42
Carousel.in :default @02:52:11.54 Carousel.home.seek 45 @02:52:11.86 Selecting Cartridge 44 @02:52:12.14 Carousel.seek 44 @02:52:12.67 Lysis1.in :fast @02:52:12.71 Lysis1.seek :locked @02:52:15.33 Plunge.seek :locked @02:52:15.49 Air.in :fast @02:52:15.55 Air.seek :locked @02:52:16.91
Carousel.reconfigure :locked @02:52:17.15 Powering cartridge bus @02:52:19.80 Gate.setIR 1750,5000,0,6 @02:52:19.93 Gate.setIR 0,5000,0,6 @02:52:19.99 PuckHeater.configure :default @02:52:20.05 Gate.power :heat,:ON,limit to 5000mA @02:52:20.66 Mounted lysis Cartridge @02:52:20.69 WARNING: Using default puck temperature sensor calibration @02:52:20.70 Using default cartridge heat loss curve for HeatingAccelInCarousel @02:52:22.25 SPR.command "read_sequences" @02:52:22.31 SPR.command "stats_on" @02:52:22.47 SPR.command "start 0 1" @02:52:23.18 SPR.start /var/log/methane/spr24mar0802h.spr.bz2 @02:52:25.12 Waiting for baseline SPR stabilization @02:52:25.15 Handoff.select :waste @02:52:31.84 Syringe tare is 35% of full scale @02:52:31.87 PinPort.in :locking @02:52:31.92 PinPort.seek :locked @02:52:32.40 Archive.in :fast @02:52:32.48 Lysis2.in :fast @02:52:32.55 Lysis1.seek 1000uL @02:52:32.62 Lysis2.seek 1000uL @02:52:32.67 Archive.seek :full @02:52:37.24 delayUntil "03:02:24.69" @03:02:24.71 Lysis2.in :DAmix @03:02:24.81 Lysis1.in :DAmix @03:02:25.39 Lysis2.seek 750uL @03:02:25.49 Lysis1.seek 750uL @03:03:00.53 delay 5 seconds @03:03:05.55 Handoff.select SPR @03:03:05.68 SPR.command "start 2 14" @03:03:06.40 SPR stabilized @03:03:06.55 delay 10 seconds @03:03:16.57 SPR.command "start 1 0" @03:03:19.77 Waiting up to 4:00 for SPR to enter state 1 @03:05:24.06 SPR.command "hold_on" @03:05:25.45 SPR Dilution 1 of 3 -- Experiment 1 with 300.0uL L1 + 300.0uL L2 @03:05:26.07 Lysis2.seek 450uL @03:05:26.12 Lysis1.seek 450uL @03:06:08.17 delay 15 seconds @03:06:23.20 SPR.command "hold_off" @03:06:26.62 Waiting up to 1:00:00 for SPR to enter state 7 @03:50:32.01 SPRsummary:-12.7RIU,134RIU,176RIU,248RIU after 3686s @03:57:09.35 delay 10 seconds @03:57:19.37 SPR.command "start 1 0" @03:57:22.09 Waiting up to 4:00 for SPR to enter state 1 @03:59:26.37 SPR.command "hold_on" @03:59:28.28 SPR Dilution 2 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @03:59:28.92 Lysis2.seek 300uL @03:59:28.96 Lysis1.seek 300uL @03:59:50.07 delay 15 seconds @04:00:05.09 SPR.command "hold_off" @04:00:07.61 Waiting up to 1:00:00 for SPR to enter state 7 @04:50:48.99 delay 10 seconds @04:50:59.01 SPR.command "start 1 0" @04:51:02.26 Waiting up to 4:00 for SPR to enter state 1 @04:53:06.95 SPR.command "hold_on" @04:53:08.33 SPR Dilution 3 of 3 -- Experiment 1 with 150.0uL L1 + 150.0uL L2 @04:53:08.89 Lysis2.seek 150uL @04:53:08.95 Lysis1.seek 150uL @04:53:30.06 delay 15 seconds @04:53:45.09 SPR.command "hold_off" @04:53:48.17 Waiting up to 1:00:00 for SPR to enter state 7 @05:44:30.37 SPR analysis completed @05:44:38.04 Ended log spr24mar0802h.spr.bz2 @05:44:38.06 SPR.command "stats_off" @05:44:38.53 Handoff.select :close @05:44:38.68
Gate.power :heat,:OFF @05:44:38.69 Gate.power :cartridge,:OFF @05:44:38.77 PinPort.in :unlocking @05:44:38.82 PinPort.seek :clear @05:44:39.27 Carousel.in :default @05:44:39.84 Carousel.home.seek 44 @05:44:40.16 Plunge.seek :locked @05:44:40.35 Archive.in :unlocking @05:44:40.47 Archive.seek :clear @05:44:40.57 Air.in :unlocking @05:44:40.72 Air.seek :clear @05:44:40.77 Lysis1.in :unlocking @05:44:40.98 Lysis1.seek :clear @05:44:41.03 Lysis2.in :unlocking @05:44:41.15 Lysis2.seek :clear @05:44:45.44
Gate.power :motor,:OFF @05:44:45.46 Gate.power :core,:OFF @05:44:45.47 Gate.power :analytic1,:OFF @05:44:45.48 Gate.power :analytic2,:OFF @05:44:45.50 Gate.power :main,:OFF @05:44:45.51 Safely stopped and ready to power off @05:44:45.69 Cartridge::None in MAIN -- Not enough standard cartridges remain @17:46:34.39PDT04-Jun-24
Environmental Sample Processor @17:46:36.99 >BREAK< ESP3 Gateway v1.43 for core bus @17:46:37.02 >BREAK< ESP3 Gateway v1.43 for cartridge bus @17:46:41.25 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @17:46:42.04 Sea pressure (Abs) tare is 6% of full scale @17:46:45.92 Polling Can every 2:00 @17:46:45.97 Loading /home/methane/esp2/bin/processAll mission @17:46:46.24 Sea pressure (Abs) tare is 6% of full scale @17:46:46.25 Sampling default goal volumes @17:46:46.34 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @17:46:46.72 Gate.power :sampler,:OFF,limit to 3500mA @17:46:46.79 Gate.power :skimmer,:OFF,limit to 3500mA @17:46:46.83 Gate.power :analytic1,:ON,limit to 4000mA @17:46:47.62 SPR Module 1, FW Rev. B, FPGA Rev. C @17:46:48.91 Session Begun @17:46:49.00
Plunge.configure :default @17:46:49.10 Plunge.hold @17:46:49.22 Plunge.configure :default @17:46:49.38 SamplePump.configure :default @17:46:49.54 Toroid.configure :default @17:46:49.66 Toroid.hold @17:46:49.75 Toroid.configure :default @17:46:49.93 Carousel.configure :default @17:46:50.02 Carousel.hold @17:46:50.11 Carousel.configure :default @17:46:50.28 Abs.configure :default @17:46:50.44 PhaseSeparator.configure :default @17:46:50.60 Archive.configure :default @17:46:50.67 Archive.hold @17:46:50.77 Archive.configure :default @17:46:50.94 Air.configure :default @17:46:51.02 Air.hold @17:46:51.12 Air.configure :default @17:46:51.26 Lysis1.configure :default @17:46:51.35 Lysis1.hold @17:46:51.45 Lysis1.configure :default @17:46:51.62 Lysis2.configure :default @17:46:51.70 Lysis2.hold @17:46:51.80 Lysis2.configure :default @17:46:51.97 PinPort.configure :default @17:46:52.05 PinPort.hold @17:46:52.14 PinPort.configure :default @17:46:52.22 All elf controllers running firmware version 4.92 @17:46:52.92 Toroid.seek :clear @17:46:53.11
Carousel.in :default @17:46:53.25 Carousel.home.seek 53 @17:46:54.02 Selecting Cartridge 50 @17:46:54.36 Carousel.seek 50 @17:46:55.32 Lysis1.in :fast @17:46:55.41 Lysis1.seek :locked @17:46:58.06 Plunge.seek :locked @17:46:58.25 Air.in :fast @17:46:58.32 Air.seek :locked @17:46:59.32
Carousel.reconfigure :locked @17:46:59.50 Powering cartridge bus @17:47:02.16 Gate.setIR 1750,5000,0,6 @17:47:02.29 Gate.setIR 0,5000,0,6 @17:47:02.38 PuckHeater.configure :default @17:47:02.48 Gate.power :heat,:ON,limit to 5000mA @17:47:03.18 Mounted lysis Cartridge @17:47:03.23 WARNING: Using default puck temperature sensor calibration @17:47:03.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:47:04.85 SPR.command "read_sequences" @17:47:05.47 SPR.command "stats_on" @17:47:05.69 SPR.command "start 0 1" @17:47:06.36 SPR.start /var/log/methane/spr24jun0417h.spr.bz2 @17:47:08.32 Waiting for baseline SPR stabilization @17:47:08.35 Handoff.select :waste @17:47:08.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:47:15.17 Syringe tare is 52% of full scale at 21C (drifted 1%) @17:47:15.21 PinPort.in :locking @17:47:15.30 PinPort.seek :locked @17:47:15.82 Archive.in :fast @17:47:15.93 Lysis2.in :fast @17:47:15.99 Lysis1.seek 1000uL @17:47:16.06 Archive.seek :full @17:47:16.24 Lysis2.seek 1000uL @17:47:20.90 delayUntil "17:57:07.88" @17:50:15.23 -> showlog @17:57:07.89 Lysis2.in :DAmix @17:57:08.03 Lysis1.in :DAmix @17:57:08.64 Lysis2.seek 750uL @17:57:08.73 Lysis1.seek 750uL @17:57:43.30 delay 5 seconds @17:57:48.32 Handoff.select SPR @17:57:48.48 SPR.command "start 2 14" @17:57:49.62 SPR stabilized @17:57:49.81 delay 10 seconds @17:57:59.84 SPR.command "start 2 0" @17:58:02.97 Waiting up to 11:00 for SPR to enter state 1 @18:00:00.64 SPR.command "hold_on" @18:00:02.03 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @18:00:02.69 Lysis2.seek 450uL @18:00:02.75 Lysis1.seek 450uL @18:00:44.26 delay 15 seconds @18:00:59.28 SPR.command "hold_off" @18:01:03.20 Waiting up to 1:00:00 for SPR to enter state 7 @18:17:19.20 SPRsummary:64.4RIU,197RIU,239RIU,202RIU after 1914s @18:28:45.98 delay 10 seconds @18:28:56.01 SPR.command "start 2 0" @18:28:58.70 Waiting up to 11:00 for SPR to enter state 1 @18:30:56.37 SPR.command "hold_on" @18:30:58.26 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:30:58.86 Lysis2.seek 300uL @18:30:58.90 Lysis1.seek 300uL @18:31:19.79 delay 15 seconds @18:31:34.81 SPR.command "hold_off" @18:31:37.62 Waiting up to 1:00:00 for SPR to enter state 7 @18:59:18.99 delay 10 seconds @18:59:29.01 SPR.command "start 2 0" @18:59:32.54 Waiting up to 11:00 for SPR to enter state 1 @19:01:30.32 SPR.command "hold_on" @19:01:31.72 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:01:32.78 Lysis2.seek 150uL @19:01:32.82 Lysis1.seek 150uL @19:01:53.68 delay 15 seconds @19:02:08.70 SPR.command "hold_off" @19:02:11.57 Waiting up to 1:00:00 for SPR to enter state 7 @19:29:53.84 Additional SPR Analyses Skipped due to Insufficient Volume @19:29:54.07 Intake.close @19:29:54.19 Exhaust.close @19:29:54.26 Bypass.close @19:29:54.32 Handoff.select :close @19:29:54.63
Gate.power :heat,:OFF @19:29:54.65 Gate.power :cartridge,:OFF @19:29:54.81 PinPort.in :unlocking @19:29:54.90 PinPort.seek :clear @19:29:55.39 Carousel.in :default @19:29:55.54 Carousel.home.seek 50 @19:29:56.38 Plunge.seek :locked @19:29:56.65 Archive.in :unlocking @19:29:56.79 Archive.seek :clear @19:29:56.95 Air.in :unlocking @19:29:57.09 Air.seek :clear @19:29:57.23 Lysis1.in :unlocking @19:29:57.41 Lysis1.seek :clear @19:29:57.52 Lysis2.in :unlocking @19:29:57.71 Lysis2.seek :clear @19:30:12.94 Ended log spr24jun0417h.spr.bz2 @19:30:12.96
SPR.command "stats_off" @19:30:13.26 Gate.power :analytic1,:OFF @19:30:13.30 Gate.power :motor,:OFF @19:30:13.32 Gate.power :core,:OFF @19:30:13.35 Gate.power :main,:OFF @19:30:13.38 Safely stopped and ready to power off @19:30:14.12 Gate.power :sampler,:OFF,limit to 3500mA @19:30:14.16 Gate.power :skimmer,:OFF,limit to 3500mA @19:30:17.16 Gate.power :main,:ON,limit to 5000mA @19:30:17.20 Gate.power :analytic1,:ON,limit to 4000mA @19:30:17.31 Powering core bus @19:30:19.66 SPR Module 1, FW Rev. B, FPGA Rev. C @19:30:20.12
Plunge.configure :default @19:30:20.24 SamplePump.configure :default @19:30:20.34 Toroid.configure :default @19:30:20.44 Carousel.configure :default @19:30:20.54 Abs.configure :default @19:30:20.65 PhaseSeparator.configure :default @19:30:20.74 Archive.configure :default @19:30:20.85 Air.configure :default @19:30:20.92 Lysis1.configure :default @19:30:21.01 Lysis2.configure :default @19:30:21.11 PinPort.configure :default @19:30:21.19 All elf controllers running firmware version 4.92 @19:30:21.22 Gate.power :motor,:ON,limit to 5000mA @19:30:21.53 Plunge.home.jog 0 @19:30:22.29 Lysis2.home.jog 0 @19:30:22.33 Air.home.jog 0 @19:30:22.38 Carousel.home.jog 0 @19:30:22.44 Lysis1.home.jog 0 @19:30:22.50 Archive.home.jog 0 @19:30:22.55 Toroid.home.jog 0 @19:30:22.59 PinPort.home.jog 0 @19:30:23.37 Plunge.in :homing @19:30:23.73 Plunge.jog 1612 @19:30:23.97 Lysis2.in :homing @19:30:24.06 Lysis1.in :homing @19:30:24.10 Archive.in :homing @19:30:24.15 PinPort.in :homing @19:30:24.20 Toroid.in :homing @19:30:24.28 Air.in :homing @19:30:25.08 Lysis2.jog 1409 @19:30:25.11 Lysis1.jog 1398 @19:30:25.19 PinPort.jog -106 @19:30:25.25 Archive.jog 1369 @19:30:25.34 Air.jog 1369 @19:30:25.41 Toroid.jog 633 @19:30:25.85 Plunge.setRawPosition! 50 @19:30:25.88 PinPort.setRawPosition! 0 @19:30:26.00 Plunge.seek :home @19:30:26.07 PinPort.seek :home @19:30:26.29 PinPort.in :default @19:30:26.41 Plunge.in :default @19:30:26.54 Lysis2.setRawPosition! 50 @19:30:26.63 Lysis2.seek :home @19:30:26.75 Lysis1.setRawPosition! 50 @19:30:26.82 Lysis1.seek :home @19:30:26.98 Air.setRawPosition! 50 @19:30:27.02 Archive.setRawPosition! 50 @19:30:27.05 Lysis2.in :default @19:30:27.25 Air.seek :home @19:30:27.30 Archive.seek :home @19:30:27.54 Lysis1.in :default @19:30:27.71 Archive.in :default @19:30:27.74 Air.in :default @19:30:27.92 Toroid.setRawPosition! 0 @19:30:27.99 Toroid.seek :home @19:30:28.11 Toroid.in :default @19:30:28.23 Toroid.seek :clear @19:30:29.99
Carousel.in :default @19:30:30.13 Carousel.home.seek 50 @19:30:30.42 Selecting Cartridge 49 @19:30:30.73 Carousel.seek 49 @19:30:31.28 Lysis1.in :fast @19:30:31.36 Lysis1.seek :locked @19:30:34.01 Plunge.seek :locked @19:30:34.19 Air.in :fast @19:30:34.27 Air.seek :locked @19:30:35.27
Carousel.reconfigure :locked @19:30:35.44 Powering cartridge bus @19:30:38.10 Gate.setIR 1750,5000,0,6 @19:30:38.22 Gate.setIR 0,5000,0,6 @19:30:38.31 PuckHeater.configure :default @19:30:38.41 Gate.power :heat,:ON,limit to 5000mA @19:30:39.04 Mounted lysis Cartridge @19:30:39.09 WARNING: Using default puck temperature sensor calibration @19:30:39.10 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:30:40.71 SPR.command "read_sequences" @19:30:41.29 SPR.command "stats_on" @19:30:42.34 SPR.command "start 0 1" @19:30:43.05 SPR.start /var/log/methane/spr24jun0419h.spr.bz2 @19:30:45.09 Waiting for baseline SPR stabilization @19:30:45.11 Handoff.select :waste @19:30:45.30 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:30:51.92 Syringe tare is 52% of full scale at 21C (drifted 1%) @19:30:51.95 PinPort.in :locking @19:30:52.05 PinPort.seek :locked @19:30:52.57 Archive.in :fast @19:30:52.66 Lysis2.in :fast @19:30:52.71 Archive.seek :full @19:30:52.84 Lysis1.seek 1000uL @19:30:52.95 Lysis2.seek 1000uL @19:30:57.66 delayUntil "19:40:44.64" @19:40:44.65 Lysis2.in :DAmix @19:40:44.80 Lysis1.in :DAmix @19:40:45.32 Lysis2.seek 750uL @19:40:45.37 Lysis1.seek 750uL @19:41:20.02 delay 5 seconds @19:41:25.05 Handoff.select SPR @19:41:25.19 SPR.command "start 2 14" @19:41:26.36 SPR stabilized @19:41:26.54 delay 10 seconds @19:41:36.56 SPR.command "start 2 0" @19:41:39.73 Waiting up to 11:00 for SPR to enter state 1 @19:43:37.39 SPR.command "hold_on" @19:43:38.79 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:43:39.42 Lysis2.seek 450uL @19:43:39.49 Lysis1.seek 450uL @19:44:21.05 delay 15 seconds @19:44:36.08 SPR.command "hold_off" @19:44:39.94 Waiting up to 1:00:00 for SPR to enter state 7 @20:00:55.94 SPRsummary:39.7RIU,263RIU,299RIU,265RIU after 1914s @20:12:22.72 delay 10 seconds @20:12:32.75 SPR.command "start 2 0" @20:12:35.46 Waiting up to 11:00 for SPR to enter state 1 @20:14:33.11 SPR.command "hold_on" @20:14:35.02 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:14:35.60 Lysis2.seek 300uL @20:14:35.70 Lysis1.seek 300uL @20:14:56.55 delay 15 seconds @20:15:11.57 SPR.command "hold_off" @20:15:14.86 Waiting up to 1:00:00 for SPR to enter state 7 @20:42:56.70 delay 10 seconds @20:43:06.72 SPR.command "start 2 0" @20:43:09.43 Waiting up to 11:00 for SPR to enter state 1 @20:45:07.07 SPR.command "hold_on" @20:45:08.48 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:45:09.17 Lysis2.seek 150uL @20:45:09.24 Lysis1.seek 150uL @20:45:30.08 delay 15 seconds @20:45:45.10 SPR.command "hold_off" @20:45:48.33 Waiting up to 1:00:00 for SPR to enter state 7 @21:13:30.60 Additional SPR Analyses Skipped due to Insufficient Volume @21:13:30.85 Handoff.select :close @21:13:31.02
Gate.power :heat,:OFF @21:13:31.06 Gate.power :cartridge,:OFF @21:13:31.16 PinPort.in :unlocking @21:13:31.25 PinPort.seek :clear @21:13:31.73 Carousel.in :default @21:13:31.88 Carousel.home.seek 49 @21:13:32.23 Plunge.seek :locked @21:13:32.57 Archive.in :unlocking @21:13:32.71 Archive.seek :clear @21:13:32.86 Air.in :unlocking @21:13:33.03 Air.seek :clear @21:13:33.10 Lysis1.in :unlocking @21:13:33.81 Lysis1.seek :clear @21:13:34.00 Lysis2.in :unlocking @21:13:34.08 Lysis2.seek :clear @21:13:49.66 Ended log spr24jun0419h.spr.bz2 @21:13:49.68
SPR.command "stats_off" @21:13:50.04 Gate.power :analytic1,:OFF @21:13:50.06 Gate.power :motor,:OFF @21:13:50.10 Gate.power :core,:OFF @21:13:50.14 Gate.power :main,:OFF @21:13:50.17 Safely stopped and ready to power off @21:13:50.48 Gate.power :sampler,:OFF,limit to 3500mA @21:13:50.50 Gate.power :skimmer,:OFF,limit to 3500mA @21:13:53.95 Gate.power :main,:ON,limit to 5000mA @21:13:53.97 Gate.power :analytic1,:ON,limit to 4000mA @21:13:54.12 Powering core bus @21:13:56.43 SPR Module 1, FW Rev. B, FPGA Rev. C @21:13:56.90
Plunge.configure :default @21:13:57.02 SamplePump.configure :default @21:13:57.12 Toroid.configure :default @21:13:57.23 Carousel.configure :default @21:13:57.34 Abs.configure :default @21:13:57.44 PhaseSeparator.configure :default @21:13:57.54 Archive.configure :default @21:13:57.67 Air.configure :default @21:13:57.75 Lysis1.configure :default @21:13:57.82 Lysis2.configure :default @21:13:57.93 PinPort.configure :default @21:13:58.00 All elf controllers running firmware version 4.92 @21:13:58.03 Gate.power :motor,:ON,limit to 5000mA @21:13:58.36 Plunge.home.jog 0 @21:13:59.06 PinPort.home.jog 0 @21:13:59.11 Lysis2.home.jog 0 @21:13:59.15 Air.home.jog 0 @21:13:59.20 Carousel.home.jog 0 @21:13:59.27 Archive.home.jog 0 @21:13:59.30 Toroid.home.jog 0 @21:13:59.35 Lysis1.home.jog 0 @21:14:00.31 Plunge.in :homing @21:14:00.48 Plunge.jog 1604 @21:14:00.69 PinPort.in :homing @21:14:00.76 Lysis2.in :homing @21:14:00.81 Air.in :homing @21:14:00.85 Toroid.in :homing @21:14:00.89 Lysis1.in :homing @21:14:00.93 Archive.in :homing @21:14:01.17 PinPort.jog -106 @21:14:01.24 Lysis2.jog 1410 @21:14:01.34 Air.jog 1368 @21:14:01.45 Archive.jog 1370 @21:14:01.51 Toroid.jog 634 @21:14:01.56 Lysis1.jog 1396 @21:14:02.04 PinPort.setRawPosition! 0 @21:14:02.07 Plunge.setRawPosition! 50 @21:14:02.22 Plunge.seek :home @21:14:02.25 PinPort.seek :home @21:14:02.47 PinPort.in :default @21:14:03.11 Lysis2.setRawPosition! 50 @21:14:03.13 Lysis1.setRawPosition! 50 @21:14:03.17 Archive.setRawPosition! 50 @21:14:03.23 Plunge.in :default @21:14:03.25 Air.setRawPosition! 50 @21:14:03.47 Lysis2.seek :home @21:14:03.50 Lysis1.seek :home @21:14:03.74 Archive.seek :home @21:14:03.81 Air.seek :home @21:14:04.16 Lysis2.in :default @21:14:04.21 Lysis1.in :default @21:14:04.24 Toroid.setRawPosition! 0 @21:14:04.28 Archive.in :default @21:14:04.52 Toroid.seek :home @21:14:04.70 Toroid.in :default @21:14:04.72 Air.in :default @21:14:05.13 Toroid.seek :clear @21:14:06.83
Carousel.in :default @21:14:06.98 Carousel.home.seek 49 @21:14:07.26 Selecting Cartridge 48 @21:14:07.61 Carousel.seek 48 @21:14:08.17 Lysis1.in :fast @21:14:08.25 Lysis1.seek :locked @21:14:10.89 Plunge.seek :locked @21:14:11.08 Air.in :fast @21:14:11.16 Air.seek :locked @21:14:12.16
Carousel.reconfigure :locked @21:14:12.33 Powering cartridge bus @21:14:14.99 Gate.setIR 1750,5000,0,6 @21:14:15.12 Gate.setIR 0,5000,0,6 @21:14:15.21 PuckHeater.configure :default @21:14:15.30 Gate.power :heat,:ON,limit to 5000mA @21:14:15.93 Mounted lysis Cartridge @21:14:15.96 WARNING: Using default puck temperature sensor calibration @21:14:16.01 Using default cartridge heat loss curve for HeatingAccelInCarousel @21:14:17.63 SPR.command "read_sequences" @21:14:18.07 SPR.command "stats_on" @21:14:18.24 SPR.command "start 0 1" @21:14:18.95 SPR.start /var/log/methane/spr24jun0421h.spr.bz2 @21:14:20.92 Waiting for baseline SPR stabilization @21:14:20.94 Handoff.select :waste @21:14:21.12 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @21:14:27.81 Syringe tare is 52% of full scale at 21C (drifted 1%) @21:14:27.84 PinPort.in :locking @21:14:27.94 PinPort.seek :locked @21:14:28.45 Archive.in :fast @21:14:28.51 Lysis2.in :fast @21:14:28.61 Lysis1.seek 1000uL @21:14:28.77 Archive.seek :full @21:14:28.95 Lysis2.seek 1000uL @21:14:33.57 delayUntil "21:24:20.47" @21:24:20.48 Lysis2.in :DAmix @21:24:20.63 Lysis1.in :DAmix @21:24:21.14 Lysis2.seek 750uL @21:24:21.25 Lysis1.seek 750uL @21:24:55.87 delay 5 seconds @21:25:00.89 Handoff.select SPR @21:25:01.04 SPR.command "start 2 14" @21:25:02.20 SPR stabilized @21:25:02.37 delay 10 seconds @21:25:12.39 SPR.command "start 2 0" @21:25:15.56 Waiting up to 11:00 for SPR to enter state 1 @21:27:13.22 SPR.command "hold_on" @21:27:15.13 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @21:27:15.77 Lysis2.seek 450uL @21:27:15.82 Lysis1.seek 450uL @21:27:57.37 delay 15 seconds @21:28:12.39 SPR.command "hold_off" @21:28:15.28 Waiting up to 1:00:00 for SPR to enter state 7 @21:44:29.88 SPRsummary:43.9RIU,271RIU,303RIU,273RIU after 1912s @21:55:56.68 delay 10 seconds @21:56:06.70 SPR.command "start 2 0" @21:56:09.91 Waiting up to 11:00 for SPR to enter state 1 @21:58:07.99 SPR.command "hold_on" @21:58:09.40 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:58:10.02 Lysis2.seek 300uL @21:58:10.09 Lysis1.seek 300uL @21:58:30.96 delay 15 seconds @21:58:45.98 SPR.command "hold_off" @21:58:49.75 Waiting up to 1:00:00 for SPR to enter state 7 @22:26:32.53 delay 10 seconds @22:26:42.55 SPR.command "start 2 0" @22:26:45.27 Waiting up to 11:00 for SPR to enter state 1 @22:28:42.92 SPR.command "hold_on" @22:28:44.32 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:28:45.02 Lysis2.seek 150uL @22:28:45.08 Lysis1.seek 150uL @22:29:05.97 delay 15 seconds @22:29:20.99 SPR.command "hold_off" @22:29:24.16 Waiting up to 1:00:00 for SPR to enter state 7 @22:57:06.43 Additional SPR Analyses Skipped due to Insufficient Volume @22:57:06.67 Handoff.select :close @22:57:06.85
Gate.power :heat,:OFF @22:57:06.89 Gate.power :cartridge,:OFF @22:57:07.00 PinPort.in :unlocking @22:57:07.08 PinPort.seek :clear @22:57:07.57 Carousel.in :default @22:57:07.74 Carousel.home.seek 48 @22:57:08.10 Plunge.seek :locked @22:57:08.35 Archive.in :unlocking @22:57:08.50 Archive.seek :clear @22:57:08.63 Air.in :unlocking @22:57:09.25 Air.seek :clear @22:57:09.39 Lysis1.in :unlocking @22:57:09.55 Lysis1.seek :clear @22:57:09.74 Lysis2.in :unlocking @22:57:09.84 Lysis2.seek :clear @22:57:25.61 Ended log spr24jun0421h.spr.bz2 @22:57:25.66
SPR.command "stats_off" @22:57:25.93 Gate.power :analytic1,:OFF @22:57:25.95 Gate.power :motor,:OFF @22:57:25.98 Gate.power :core,:OFF @22:57:26.02 Gate.power :main,:OFF @22:57:26.05 Safely stopped and ready to power off @22:57:26.29 Gate.power :sampler,:OFF,limit to 3500mA @22:57:26.31 Gate.power :skimmer,:OFF,limit to 3500mA @22:57:29.83 Gate.power :main,:ON,limit to 5000mA @22:57:29.85 Gate.power :analytic1,:ON,limit to 4000mA @22:57:30.00 Powering core bus @22:57:32.31 SPR Module 1, FW Rev. B, FPGA Rev. C @22:57:32.78
Plunge.configure :default @22:57:32.88 SamplePump.configure :default @22:57:32.99 Toroid.configure :default @22:57:33.10 Carousel.configure :default @22:57:33.20 Abs.configure :default @22:57:33.30 PhaseSeparator.configure :default @22:57:33.40 Archive.configure :default @22:57:33.49 Air.configure :default @22:57:33.57 Lysis1.configure :default @22:57:33.69 Lysis2.configure :default @22:57:33.79 PinPort.configure :default @22:57:33.87 All elf controllers running firmware version 4.92 @22:57:33.89 Gate.power :motor,:ON,limit to 5000mA @22:57:34.15 Plunge.home.jog 0 @22:57:34.82 Lysis2.home.jog 0 @22:57:34.99 Lysis1.home.jog 0 @22:57:35.03 Archive.home.jog 0 @22:57:35.08 Toroid.home.jog 0 @22:57:35.14 Carousel.home.jog 0 @22:57:35.20 PinPort.home.jog 0 @22:57:35.25 Air.home.jog 0 @22:57:36.13 Plunge.in :homing @22:57:36.49 Plunge.jog 1607 @22:57:36.57 PinPort.in :homing @22:57:36.63 Lysis1.in :homing @22:57:36.68 Archive.in :homing @22:57:36.73 Air.in :homing @22:57:36.76 Toroid.in :homing @22:57:36.80 Lysis2.in :homing @22:57:37.13 Lysis1.jog 1395 @22:57:37.24 PinPort.jog -106 @22:57:37.28 Archive.jog 1370 @22:57:37.35 Toroid.jog 634 @22:57:37.38 Air.jog 1370 @22:57:37.44 Lysis2.jog 1409 @22:57:37.96 PinPort.setRawPosition! 0 @22:57:38.58 PinPort.seek :home @22:57:38.72 Lysis1.setRawPosition! 50 @22:57:38.76 Plunge.setRawPosition! 50 @22:57:38.81 PinPort.in :default @22:57:39.00 Lysis1.seek :home @22:57:39.05 Plunge.seek :home @22:57:39.30 Air.setRawPosition! 50 @22:57:39.35 Archive.setRawPosition! 50 @22:57:39.38 Lysis2.setRawPosition! 50 @22:57:39.57 Air.seek :home @22:57:39.66 Archive.seek :home @22:57:39.70 Lysis2.seek :home @22:57:40.02 Lysis1.in :default @22:57:40.08 Toroid.setRawPosition! 0 @22:57:40.19 Plunge.in :default @22:57:40.30 Toroid.seek :home @22:57:40.48 Air.in :default @22:57:40.53 Toroid.in :default @22:57:40.57 Lysis2.in :default @22:57:40.65 Archive.in :default @22:57:41.14 Toroid.seek :clear @22:57:42.82
Carousel.in :default @22:57:42.97 Carousel.home.seek 48 @22:57:43.25 Selecting Cartridge 47 @22:57:43.57 Carousel.seek 47 @22:57:44.16 Lysis1.in :fast @22:57:44.25 Lysis1.seek :locked @22:57:46.90 Plunge.seek :locked @22:57:47.09 Air.in :fast @22:57:47.16 Air.seek :locked @22:57:48.15
Carousel.reconfigure :locked @22:57:48.32 Powering cartridge bus @22:57:50.99 Gate.setIR 1750,5000,0,6 @22:57:51.11 Gate.setIR 0,5000,0,6 @22:57:51.20 PuckHeater.configure :default @22:57:51.30 Gate.power :heat,:ON,limit to 5000mA @22:57:51.92 Mounted lysis Cartridge @22:57:51.97 WARNING: Using default puck temperature sensor calibration @22:57:51.99 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:57:53.58 SPR.command "read_sequences" @22:57:53.96 SPR.command "stats_on" @22:57:54.59 SPR.command "start 0 1" @22:57:54.84 SPR.start /var/log/methane/spr24jun0422h.spr.bz2 @22:57:56.81 Waiting for baseline SPR stabilization @22:57:56.83 Handoff.select :waste @22:57:57.02 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:58:03.67 Syringe tare is 52% of full scale at 21C (drifted 1%) @22:58:03.72 PinPort.in :locking @22:58:03.80 PinPort.seek :locked @22:58:04.30 Archive.in :fast @22:58:04.43 Lysis2.in :fast @22:58:04.47 Lysis1.seek 1000uL @22:58:04.52 Archive.seek :full @22:58:04.70 Lysis2.seek 1000uL @22:58:09.38 delayUntil "23:07:56.36" @23:07:56.38 Lysis2.in :DAmix @23:07:56.52 Lysis1.in :DAmix @23:07:57.09 Lysis2.seek 750uL @23:07:57.13 Lysis1.seek 750uL @23:08:31.74 delay 5 seconds @23:08:36.77 Handoff.select SPR @23:08:36.93 SPR.command "start 2 14" @23:08:38.08 SPR stabilized @23:08:38.27 delay 10 seconds @23:08:48.29 SPR.command "start 2 0" @23:08:51.96 Waiting up to 11:00 for SPR to enter state 1 @23:10:50.05 SPR.command "hold_on" @23:10:51.45 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @23:10:52.15 Lysis2.seek 450uL @23:10:52.21 Lysis1.seek 450uL @23:11:33.75 delay 15 seconds @23:11:48.77 SPR.command "hold_off" @23:11:52.11 Waiting up to 1:00:00 for SPR to enter state 7 @23:28:07.69 SPRsummary:33.7RIU,214RIU,239RIU,220RIU after 1914s @23:39:34.45 delay 10 seconds @23:39:44.47 SPR.command "start 2 0" @23:39:47.70 Waiting up to 11:00 for SPR to enter state 1 @23:41:45.78 SPR.command "hold_on" @23:41:47.18 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:41:47.83 Lysis2.seek 300uL @23:41:47.93 Lysis1.seek 300uL @23:42:08.76 delay 15 seconds @23:42:23.79 SPR.command "hold_off" @23:42:27.02 Waiting up to 1:00:00 for SPR to enter state 7 @00:10:09.36PDT05-Jun-24 delay 10 seconds @00:10:19.38 SPR.command "start 2 0" @00:10:22.09 Waiting up to 11:00 for SPR to enter state 1 @00:12:19.74 SPR.command "hold_on" @00:12:21.15 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @00:12:21.89 Lysis2.seek 150uL @00:12:21.93 Lysis1.seek 150uL @00:12:42.81 delay 15 seconds @00:12:57.84 SPR.command "hold_off" @00:13:01.00 Waiting up to 1:00:00 for SPR to enter state 7 @00:40:43.27 Additional SPR Analyses Skipped due to Insufficient Volume @00:40:43.52 Handoff.select :close @00:40:43.72
Gate.power :heat,:OFF @00:40:43.76 Gate.power :cartridge,:OFF @00:40:43.88 PinPort.in :unlocking @00:40:43.96 PinPort.seek :clear @00:40:44.45 Carousel.in :default @00:40:44.59 Carousel.home.seek 47 @00:40:45.42 Plunge.seek :locked @00:40:45.64 Archive.in :unlocking @00:40:45.79 Archive.seek :clear @00:40:45.93 Air.in :unlocking @00:40:46.10 Air.seek :clear @00:40:46.24 Lysis1.in :unlocking @00:40:46.39 Lysis1.seek :clear @00:40:46.53 Lysis2.in :unlocking @00:40:46.63 Lysis2.seek :clear @00:41:01.36 Ended log spr24jun0422h.spr.bz2 @00:41:01.38
SPR.command "stats_off" @00:41:01.78 Gate.power :analytic1,:OFF @00:41:01.80 Gate.power :motor,:OFF @00:41:01.83 Gate.power :core,:OFF @00:41:01.88 Gate.power :main,:OFF @00:41:01.92 Safely stopped and ready to power off @00:41:02.22 Gate.power :sampler,:OFF,limit to 3500mA @00:41:02.24 Gate.power :skimmer,:OFF,limit to 3500mA @00:41:05.69 Gate.power :main,:ON,limit to 5000mA @00:41:05.71 Gate.power :analytic1,:ON,limit to 4000mA @00:41:05.84 Powering core bus @00:41:08.19 SPR Module 1, FW Rev. B, FPGA Rev. C @00:41:08.62
Plunge.configure :default @00:41:08.73 SamplePump.configure :default @00:41:08.84 Toroid.configure :default @00:41:08.94 Carousel.configure :default @00:41:09.05 Abs.configure :default @00:41:09.16 PhaseSeparator.configure :default @00:41:09.26 Archive.configure :default @00:41:09.37 Air.configure :default @00:41:09.45 Lysis1.configure :default @00:41:09.52 Lysis2.configure :default @00:41:09.66 PinPort.configure :default @00:41:09.73 All elf controllers running firmware version 4.92 @00:41:09.76 Gate.power :motor,:ON,limit to 5000mA @00:41:10.03 Plunge.home.jog 0 @00:41:10.83 Lysis2.home.jog 0 @00:41:10.89 Air.home.jog 0 @00:41:10.94 Carousel.home.jog 0 @00:41:10.98 Lysis1.home.jog 0 @00:41:11.04 Archive.home.jog 0 @00:41:11.09 Toroid.home.jog 0 @00:41:11.14 PinPort.home.jog 0 @00:41:12.48 Plunge.in :homing @00:41:12.91 Plunge.jog 1603 @00:41:13.00 Lysis2.in :homing @00:41:13.05 Air.in :homing @00:41:13.10 Toroid.in :homing @00:41:13.14 PinPort.in :homing @00:41:13.19 Archive.in :homing @00:41:13.25 Lysis1.in :homing @00:41:13.60 Lysis2.jog 1409 @00:41:13.68 Archive.jog 1369 @00:41:13.73 PinPort.jog -104 @00:41:13.78 Air.jog 1371 @00:41:13.82 Lysis1.jog 1396 @00:41:13.88 Toroid.jog 624 @00:41:14.37 PinPort.setRawPosition! 0 @00:41:14.44 PinPort.seek :home @00:41:14.60 Plunge.setRawPosition! 50 @00:41:14.63 PinPort.in :default @00:41:14.77 Plunge.seek :home @00:41:15.08 Plunge.in :default @00:41:15.19 Lysis2.setRawPosition! 50 @00:41:15.25 Archive.setRawPosition! 50 @00:41:15.37 Lysis2.seek :home @00:41:15.44 Archive.seek :home @00:41:15.66 Lysis1.setRawPosition! 50 @00:41:15.68 Air.setRawPosition! 50 @00:41:15.85 Lysis1.seek :home @00:41:15.88 Air.seek :home @00:41:16.09 Lysis2.in :default @00:41:16.12 Archive.in :default @00:41:16.31 Lysis1.in :default @00:41:16.37 Air.in :default @00:41:16.54 Toroid.setRawPosition! 0 @00:41:16.63 Toroid.seek :home @00:41:16.75 Toroid.in :default @00:41:16.88 Toroid.seek :clear @00:41:18.58
Carousel.in :default @00:41:18.73 Carousel.home.seek 47 @00:41:19.03 Selecting Cartridge 46 @00:41:19.34 Carousel.seek 46 @00:41:19.89 Lysis1.in :fast @00:41:19.97 Lysis1.seek :locked @00:41:22.63 Plunge.seek :locked @00:41:22.82 Air.in :fast @00:41:22.90 Air.seek :locked @00:41:23.90
Carousel.reconfigure :locked @00:41:24.48 Powering cartridge bus @00:41:27.24 Gate.setIR 1750,5000,0,6 @00:41:27.36 Gate.setIR 0,5000,0,6 @00:41:27.46 PuckHeater.configure :default @00:41:27.54 Gate.power :heat,:ON,limit to 5000mA @00:41:28.17 Mounted lysis Cartridge @00:41:28.23 WARNING: Using default puck temperature sensor calibration @00:41:28.25 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:41:29.89 SPR.command "read_sequences" @00:41:29.97 SPR.command "stats_on" @00:41:30.87 SPR.command "start 0 1" @00:41:31.57 SPR.start /var/log/methane/spr24jun0500h.spr.bz2 @00:41:33.62 Waiting for baseline SPR stabilization @00:41:33.66 Handoff.select :waste @00:41:33.85 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:41:40.47 Syringe tare is 52% of full scale at 21C (drifted 1%) @00:41:40.50 PinPort.in :locking @00:41:40.61 PinPort.seek :locked @00:41:41.13 Archive.in :fast @00:41:41.24 Lysis2.in :fast @00:41:41.30 Lysis1.seek 1000uL @00:41:41.35 Archive.seek :full @00:41:41.58 Lysis2.seek 1000uL @00:41:46.31 delayUntil "00:51:33.16" @00:51:33.17 Lysis2.in :DAmix @00:51:33.32 Lysis1.in :DAmix @00:51:33.94 Lysis2.seek 750uL @00:51:34.02 Lysis1.seek 750uL @00:52:08.66 delay 5 seconds @00:52:13.68 Handoff.select SPR @00:52:13.83 SPR.command "start 2 14" @00:52:14.89 SPR stabilized @00:52:15.07 delay 10 seconds @00:52:25.09 SPR.command "start 2 0" @00:52:28.25 Waiting up to 11:00 for SPR to enter state 1 @00:54:25.91 SPR.command "hold_on" @00:54:27.31 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:54:28.00 Lysis2.seek 450uL @00:54:28.04 Lysis1.seek 450uL @00:55:09.58 delay 15 seconds @00:55:24.60 SPR.command "hold_off" @00:55:27.96 Waiting up to 1:00:00 for SPR to enter state 7 @01:00:25.29 AnalyticModule::ReadTimeout in SPRlogger -- No response from SPR on AMspr @01:00:25.35 Thread::ChildDied in PROCESSING -- :SPRlogger @01:00:25.37 Waiting to Resume from Checkpoint "Waiting up to 1:00:00 for SPR to enter state 7" @10:06:10.37 -> SC Storage Carousel (SC) at 46 @10:06:20.89 -> Cartridge.unlock @10:06:20.95 Gate.power :heat,:OFF @10:06:20.97 Gate.power :cartridge,:OFF @10:06:21.11 PinPort.in :unlocking @10:06:21.19 PinPort.seek :clear @10:06:21.73 Carousel.in :default @10:06:21.87 Carousel.home.seek 46 @10:06:22.25 Archive.in :unlocking @10:06:22.39 Archive.seek :clear @10:06:22.52 Air.in :unlocking @10:06:22.68 Air.seek :clear @10:06:22.77 Lysis2.in :unlocking @10:06:22.90 Lysis2.seek :clear @10:06:26.59 Lysis1.in :unlocking @10:06:26.73 Lysis1.seek :clear @10:06:26.86 Plunge.in :unlocking @10:06:26.95 Plunge.seek :clear @10:06:42.75 -> slot {45..40 => { Type: :microSPR_phyto, State: :standard, lysed: 1000, lysed2: 1000}, 46 => { Type: :microSPR_phyto, State: :processing, lysed: 1000, lysed2: 1000, spr: spr24jun0500h.spr.bz2}, 47 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr24jun0422h.spr.bz2}, 48 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr24jun0421h.spr.bz2}, 49 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr24jun0419h.spr.bz2}, 50 => { Type: :microSPR_phyto, State: :processed, lysed: 1000, lysed2: 1000, spr: spr24jun0417h.spr.bz2}, 53 => { Type: :archive_bac, State: :missing}} @10:08:59.27 Session Begun @10:09:39.83 -> SPR.start @10:09:39.88 SPR.command "stats_on" @10:09:42.15 AnalyticModule::ReadTimeout in bill#2 -- No response from SPR on AMspr (try 1 of 3) @10:09:45.76 AnalyticModule::ReadTimeout in bill#2 -- No response from SPR on AMspr (try 2 of 3) @10:09:49.35 AnalyticModule::ReadTimeout in bill#2 -- No response from SPR on AMspr @10:09:56.82 -> ESP.ready! @10:09:58.37 Toroid.seek :clear @10:09:58.61 Carousel.in :default @10:09:58.77 Carousel.home.seek 46 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects Analytical Module SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 46 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @-0.39psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:10:03.61 -> SC Storage Carousel (SC) at 46 @10:11:44.79 -> Power {analytic1: true, cartridge: false, core: true, heat: false, main: true, motor: true, sampler: false, skimmer: false} @10:32:39.07 -> ESP,shutdown @10:32:39.13 SyntaxError in bill#2 -- compile error bill#2:5: syntax error, unexpected $end, expecting '=' ESP,shutdown ^ @10:32:48.35 -> esp :esp @10:32:51.08 -> espclient :espclient @10:32:56.19 -> ESP.ready! @10:32:57.07 Toroid.seek :clear @10:32:57.30 Carousel.in :default @10:32:57.45 Carousel.home.seek 46 Plunge = Plunge Valve (PV) clear Handoff Valve::Manifold selects Analytical Module SamplePump = Sample Pump (SP) Not yet Homed! Intake is closed Exhaust is closed Bypass = Bypass (PB) is closed Toroid = Toroid Valve (TV) clear Carousel = Storage Carousel (SC) at 46 Abs = Absolute Pressure (Leak) Sensor @15psia PhaseSeparator = Intake Head Pressure @-0.39psi Archive = Archive Syringe (ArS) clear Air = Air Syringe (AirS) clear Lysis1 = Lysis Syringe #1 (LyS1) clear Lysis2 = Lysis Syringe #2 (LyS2) clear PinPort = Handoff Pin Port (PP) clear @10:33:04.53 -> ESP.shutdown @10:33:34.58 -> MainThread.exit # @10:33:34.83 Handoff.select :close @10:33:34.96 Handoff.select :close @10:33:35.24 Session Ended @10:33:35.29 Session Ended @12:01:44.19PDT11-Jun-24
Environmental Sample Processor @12:01:46.14 >BREAK< ESP3 Gateway v1.43 for core bus @12:01:46.17 >BREAK< ESP3 Gateway v1.43 for cartridge bus @12:01:50.36 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @12:01:51.10 Sea pressure (Abs) tare is 6% of full scale @12:01:55.02 Polling Can every 2:00 @12:01:55.07 Loading /home/methane/esp2/bin/processAll mission @12:01:55.33 Sea pressure (Abs) tare is 6% of full scale @12:01:55.34 Sampling default goal volumes @12:01:55.36 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @12:01:55.76 Gate.power :sampler,:OFF,limit to 3500mA @12:01:55.78 Gate.power :skimmer,:OFF,limit to 3500mA @12:01:55.82 Gate.power :analytic1,:ON,limit to 4000mA @12:01:56.91 SPR Module 1, FW Rev. B, FPGA Rev. C @12:01:58.11 Session Begun @12:01:58.20
Plunge.configure :default @12:01:58.41 SamplePump.configure :default @12:01:58.58 Toroid.configure :default @12:01:58.66 Toroid.hold @12:01:58.76 Toroid.configure :default @12:01:58.92 Carousel.configure :default @12:01:59.00 Carousel.hold @12:01:59.10 Carousel.configure :default @12:01:59.25 Abs.configure :default @12:01:59.42 PhaseSeparator.configure :default @12:01:59.64 Archive.configure :default @12:01:59.81 Air.configure :default @12:01:59.96 Lysis1.configure :default @12:02:00.11 Lysis2.configure :default @12:02:00.28 PinPort.configure :default @12:02:00.36 All elf controllers running firmware version 4.92 @12:02:01.08 Toroid.seek :clear @12:02:01.30
Carousel.in :default @12:02:01.49 Carousel.home.seek 54 @12:02:01.79 Selecting Cartridge 50 @12:02:02.12 Carousel.seek 50 @12:02:03.75 Lysis1.in :fast @12:02:03.83 Lysis1.seek :locked @12:02:06.52 Plunge.seek :locked @12:02:06.73 Air.in :fast @12:02:06.81 Air.seek :locked @12:02:07.79
Carousel.reconfigure :locked @12:02:07.97 Powering cartridge bus @12:02:10.63 Gate.setIR 1750,5000,0,6 @12:02:10.76 Gate.setIR 0,5000,0,6 @12:02:10.85 PuckHeater.configure :default @12:02:10.95 Gate.power :heat,:ON,limit to 5000mA @12:02:11.64 Mounted lysis Cartridge @12:02:11.67 WARNING: Using default puck temperature sensor calibration @12:02:11.71 Using default cartridge heat loss curve for HeatingAccelInCarousel @12:02:13.29 SPR.command "read_sequences" @12:02:13.84 SPR.command "stats_on" @12:02:14.03 SPR.command "start 0 1" @12:02:14.73 SPR.start /var/log/methane/spr24jun1112h.spr.bz2 @12:02:16.70 Waiting for baseline SPR stabilization @12:02:16.72 Handoff.select :waste @12:02:16.90 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @12:02:23.55 Syringe tare is 52% of full scale at 22C (drifted 1%) @12:02:23.59 PinPort.in :locking @12:02:23.70 PinPort.seek :locked @12:02:24.20 Archive.in :fast @12:02:24.35 Lysis2.in :fast @12:02:24.40 Lysis1.seek 1100uL @12:02:24.45 Archive.seek :full @12:02:24.66 Lysis2.seek 1100uL @12:02:28.95 delayUntil "12:12:16.23" @12:12:16.25 Lysis2.in :DAmix @12:12:16.40 Lysis1.in :DAmix @12:12:16.99 Lysis2.seek 850uL @12:12:17.07 Lysis1.seek 850uL @12:12:51.66 delay 5 seconds @12:12:56.68 Handoff.select SPR @12:12:56.84 SPR.command "start 2 14" @12:12:57.97 SPR stabilized @12:12:58.15 delay 10 seconds @12:13:08.17 SPR.command "start 2 0" @12:13:11.35 Waiting up to 11:00 for SPR to enter state 1 @12:15:08.99 SPR.command "hold_on" @12:15:10.39 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @12:15:11.03 Lysis2.seek 550uL @12:15:11.16 Lysis1.seek 550uL @12:15:52.58 delay 15 seconds @12:16:07.60 SPR.command "hold_off" @12:16:11.55 Waiting up to 1:00:00 for SPR to enter state 7 @12:26:12.20 Session Begun @12:26:16.05 -> showlog @12:32:27.55 SPRsummary:-1e+04RIU,194RIU,3.2e+08RIU,1.49e+09RIU after 1914s @12:43:54.34 delay 10 seconds @12:44:04.36 SPR.command "start 2 0" @12:44:07.07 Waiting up to 11:00 for SPR to enter state 1 @12:46:04.72 SPR.command "hold_on" @12:46:06.12 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @12:46:06.73 Lysis2.seek 400uL @12:46:06.79 Lysis1.seek 400uL @12:46:27.70 delay 15 seconds @12:46:42.72 SPR.command "hold_off" @12:46:45.96 Waiting up to 1:00:00 for SPR to enter state 7 @13:14:28.32 delay 10 seconds @13:14:38.34 SPR.command "start 2 0" @13:14:41.03 Waiting up to 11:00 for SPR to enter state 1 @13:16:38.69 SPR.command "hold_on" @13:16:40.09 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @13:16:40.80 Lysis2.seek 250uL @13:16:40.87 Lysis1.seek 250uL @13:17:01.78 delay 15 seconds @13:17:16.80 SPR.command "hold_off" @13:17:19.93 Waiting up to 1:00:00 for SPR to enter state 7 @13:45:02.29 delay 10 seconds @13:45:12.31 SPR.command "start 2 0" @13:45:15.00 Waiting up to 11:00 for SPR to enter state 1 @13:47:12.67 SPR.command "hold_on" @13:47:14.05 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @13:47:14.80 Lysis2.seek 100uL @13:47:14.86 Lysis1.seek 100uL @13:47:35.75 delay 15 seconds @13:47:50.77 SPR.command "hold_off" @13:47:53.90 Waiting up to 1:00:00 for SPR to enter state 7 @14:01:23.10 -> MainThread.exit # @14:01:25.47 Ended log spr24jun1112h.spr.bz2 @14:01:25.51
SPR.command "stats_off" @14:01:26.65 Intake.close @14:01:26.73 Exhaust.close @14:01:26.80 Bypass.close @14:01:26.86 Handoff.select :close @14:01:27.84 Session Ended @14:01:27.90 Session Ended @15:11:49.55PDT11-Jun-24
Environmental Sample Processor @15:11:51.54 >BREAK< ESP3 Gateway v1.43 for core bus @15:11:51.58 >BREAK< ESP3 Gateway v1.43 for cartridge bus @15:11:55.82 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @15:11:56.56 Sea pressure (Abs) tare is 6% of full scale @15:12:00.50 Polling Can every 2:00 @15:12:00.54 Loading /home/methane/esp2/bin/processAll mission @15:12:00.80 Sea pressure (Abs) tare is 6% of full scale @15:12:00.82 Sampling default goal volumes @15:12:00.85 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @15:12:01.01 Gate.power :sampler,:OFF,limit to 3500mA @15:12:01.05 Gate.power :skimmer,:OFF,limit to 3500mA @15:12:01.07 Gate.power :analytic1,:ON,limit to 4000mA @15:12:01.85 SPR Module 1, FW Rev. B, FPGA Rev. C @15:12:03.56 Session Begun @15:12:03.70
Plunge.configure :default @15:12:03.79 Plunge.hold @15:12:03.89 Plunge.configure :default @15:12:04.06 SamplePump.configure :default @15:12:04.22 Toroid.configure :default @15:12:04.30 Toroid.hold @15:12:04.39 Toroid.configure :default @15:12:04.56 Carousel.configure :default @15:12:04.64 Carousel.hold @15:12:04.74 Carousel.configure :default @15:12:04.90 Abs.configure :default @15:12:05.07 PhaseSeparator.configure :default @15:12:05.23 Archive.configure :default @15:12:05.32 Archive.hold @15:12:05.42 Archive.configure :default @15:12:05.61 Air.configure :default @15:12:05.69 Air.hold @15:12:05.79 Air.configure :default @15:12:05.94 Lysis1.configure :default @15:12:06.02 Lysis1.hold @15:12:06.12 Lysis1.configure :default @15:12:06.27 Lysis2.configure :default @15:12:06.34 Lysis2.hold @15:12:06.44 Lysis2.configure :default @15:12:06.61 PinPort.configure :default @15:12:06.69 PinPort.hold @15:12:06.79 PinPort.configure :default @15:12:06.87 All elf controllers running firmware version 4.92 @15:12:07.73 Toroid.seek :clear @15:12:08.37
Carousel.in :default @15:12:08.51 Carousel.home.seek 50 @15:12:08.80 Selecting Cartridge 50 @15:12:09.12 Carousel.seek 50 @15:12:09.40 Lysis1.in :fast @15:12:09.48 Lysis1.seek :locked @15:12:12.18 Plunge.seek :locked @15:12:12.38 Air.in :fast @15:12:12.47 Air.seek :locked @15:12:13.47
Carousel.reconfigure :locked @15:12:13.68 Powering cartridge bus @15:12:20.34 Gate.power :cartridge,:OFF @15:12:20.38 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @15:12:20.42 Waiting to Resume from Checkpoint "Selecting Cartridge 50" @15:12:39.33 -> Cartridge.unmount @15:12:39.44 Carousel.in :default @15:12:39.61 Carousel.home.seek 50 @15:12:40.04 Air.in :unlocking @15:12:40.19 Air.seek :clear @15:12:41.23 Lysis1.in :unlocking @15:12:41.38 Lysis1.seek :clear @15:12:41.54 Plunge.in :unlocking @15:12:41.64 Plunge.seek :clear @15:12:46.99 -> exit @15:12:47.02 Session Ended @15:13:11.03 Session Begun @15:13:14.12 -> slot {50..40 => { Type: :microSPR_phyto, State: :standard, lysed: 1100, lysed2: 1100}} @15:13:21.38 -> MainThread.exti @15:13:21.42 NoMethodError in bill -- undefined method `exti' for # @15:13:24.96 -> MainThread.exit @15:13:25.07 Intake.close @15:13:25.17 Bypass.close @15:13:25.22 Exhaust.close # @15:13:25.64 Session Ended @15:14:35.56PDT11-Jun-24
Environmental Sample Processor @15:14:37.44 >BREAK< ESP3 Gateway v1.43 for core bus @15:14:37.47 >BREAK< ESP3 Gateway v1.43 for cartridge bus @15:14:41.59 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @15:14:42.32 Sea pressure (Abs) tare is 6% of full scale @15:14:46.24 Polling Can every 2:00 @15:14:46.30 Loading /home/methane/esp2/bin/processAll mission @15:14:46.57 Sea pressure (Abs) tare is 6% of full scale @15:14:46.58 Sampling default goal volumes @15:14:46.60 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @15:14:46.79 Gate.power :sampler,:OFF,limit to 3500mA @15:14:46.81 Gate.power :skimmer,:OFF,limit to 3500mA @15:14:46.82 Gate.power :analytic1,:ON,limit to 4000mA @15:14:48.19 SPR Module 1, FW Rev. B, FPGA Rev. C @15:14:49.32 Session Begun @15:14:49.43
Plunge.configure :default @15:14:49.66 SamplePump.configure :default @15:14:49.83 Toroid.configure :default @15:14:49.91 Toroid.hold @15:14:50.02 Toroid.configure :default @15:14:50.19 Carousel.configure :default @15:14:50.27 Carousel.hold @15:14:50.36 Carousel.configure :default @15:14:50.52 Abs.configure :default @15:14:50.68 PhaseSeparator.configure :default @15:14:50.85 Archive.configure :default @15:14:51.01 Air.configure :default @15:14:51.15 Lysis1.configure :default @15:14:51.29 Lysis2.configure :default @15:14:51.45 PinPort.configure :default @15:14:51.57 All elf controllers running firmware version 4.92 @15:14:52.36 Toroid.seek :clear @15:14:52.55
Carousel.in :default @15:14:52.70 Carousel.home.seek 50 @15:14:53.00 Selecting Cartridge 50 @15:14:53.31 Carousel.seek 50 @15:14:54.01 Lysis1.in :fast @15:14:54.09 Lysis1.seek :locked @15:14:56.82 Plunge.seek :locked @15:14:57.04 Air.in :fast @15:14:57.12 Air.seek :locked @15:14:58.12
Carousel.reconfigure :locked @15:14:58.31 Powering cartridge bus @15:15:04.98 Gate.power :cartridge,:OFF @15:15:05.04 I2C::SoftErr in MAIN -- Bus Error writing to 0x00 @15:15:05.06 Waiting to Resume from Checkpoint "Selecting Cartridge 50" @15:15:20.06 -> exit @15:15:20.09 Session Ended @15:19:12.20PDT11-Jun-24
Environmental Sample Processor @15:19:14.23 >BREAK< ESP3 Gateway v1.43 for core bus @15:19:14.26 >BREAK< ESP3 Gateway v1.43 for cartridge bus @15:19:18.59 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @15:19:19.34 Sea pressure (Abs) tare is 6% of full scale @15:19:23.33 Polling Can every 2:00 @15:19:23.37 Loading /home/methane/esp2/bin/processAll mission @15:19:23.65 Sea pressure (Abs) tare is 6% of full scale @15:19:23.66 Sampling default goal volumes @15:19:23.69 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @15:19:23.74 Gate.power :sampler,:OFF,limit to 3500mA @15:19:23.75 Gate.power :skimmer,:OFF,limit to 3500mA @15:19:23.77 Gate.power :main,:ON,limit to 5000mA @15:19:23.78 Gate.power :analytic1,:ON,limit to 4000mA @15:19:23.97 Powering core bus @15:19:26.04 Session Begun @15:19:27.12 SPR Module 1, FW Rev. B, FPGA Rev. C @15:19:27.81
Plunge.configure :default @15:19:27.91 SamplePump.configure :default @15:19:28.01 Toroid.configure :default @15:19:28.11 Carousel.configure :default @15:19:28.20 Abs.configure :default @15:19:28.31 PhaseSeparator.configure :default @15:19:28.41 Archive.configure :default @15:19:28.50 Air.configure :default @15:19:28.62 Lysis1.configure :default @15:19:28.72 Lysis2.configure :default @15:19:28.82 PinPort.configure :default @15:19:28.90 All elf controllers running firmware version 4.92 @15:19:28.95 Gate.power :motor,:ON,limit to 5000mA @15:19:29.40 Plunge.home.jog 0 @15:19:29.95 PinPort.home.jog 0 @15:19:30.00 Lysis2.home.jog 0 @15:19:30.04 Air.home.jog 0 @15:19:30.08 Carousel.home.jog 0 @15:19:30.16 Archive.home.jog 0 @15:19:30.20 Lysis1.home.jog 0 @15:19:30.27 Toroid.home.jog 0 @15:19:31.07 Plunge.in :homing @15:19:31.32 Plunge.jog 1615 @15:19:31.40 Lysis2.in :homing @15:19:31.45 Lysis1.in :homing @15:19:31.50 Air.in :homing @15:19:31.54 Toroid.in :homing @15:19:31.57 PinPort.in :homing @15:19:31.64 Archive.in :homing @15:19:31.93 Lysis2.jog 1406 @15:19:32.13 PinPort.jog -106 @15:19:32.18 Lysis1.jog 2133 @15:19:32.24 Archive.jog 1369 @15:19:32.28 Toroid.jog 636 @15:19:32.33 Air.jog 1144 @15:19:33.12 PinPort.setRawPosition! 0 @15:19:33.16 Plunge.setRawPosition! 50 @15:19:33.26 PinPort.seek :home @15:19:33.34 Plunge.seek :home @15:19:33.60 Lysis2.setRawPosition! 50 @15:19:33.65 PinPort.in :default @15:19:33.83 Lysis2.seek :home @15:19:33.98 Air.setRawPosition! 50 @15:19:34.03 Archive.setRawPosition! 50 @15:19:34.08 Lysis1.setRawPosition! 50 @15:19:34.10 Plunge.in :default @15:19:34.34 Air.seek :home @15:19:34.39 Archive.seek :home @15:19:34.44 Lysis1.seek :home @15:19:34.75 Toroid.setRawPosition! 0 @15:19:34.78 Lysis2.in :default @15:19:34.95 Toroid.seek :home @15:19:35.12 Air.in :default @15:19:35.17 Archive.in :default @15:19:35.23 Lysis1.in :default @15:19:35.25 Toroid.in :default @15:19:35.75 Toroid.seek :clear @15:19:37.50
Carousel.in :default @15:19:37.65 Carousel.home.seek 50 @15:19:37.93 Selecting Cartridge 50 @15:19:38.25 Carousel.seek 50 @15:19:38.52 Lysis1.in :fast @15:19:38.63 Lysis1.seek :locked @15:19:41.31 Plunge.seek :locked @15:19:41.50 Air.in :fast @15:19:41.59 Air.seek :locked @15:19:42.58
Carousel.reconfigure :locked @15:19:42.77 Powering cartridge bus @15:19:45.42 Gate.setIR 1750,5000,0,6 @15:19:45.55 Gate.setIR 0,5000,0,6 @15:19:45.64 PuckHeater.configure :default @15:19:45.73 Gate.power :heat,:ON,limit to 5000mA @15:19:46.41 Mounted lysis Cartridge @15:19:46.46 WARNING: Using default puck temperature sensor calibration @15:19:46.49 Using default cartridge heat loss curve for HeatingAccelInCarousel @15:19:48.08 SPR.command "read_sequences" @15:19:48.80 SPR.command "stats_on" @15:19:48.98 SPR.command "start 0 1" @15:19:49.68 SPR.start /var/log/methane/spr24jun1115h.spr.bz2 @15:19:51.66 Waiting for baseline SPR stabilization @15:19:51.67 Handoff.select :waste @15:19:51.87 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @15:19:58.50 Syringe tare is 52% of full scale at 22C (drifted 1%) @15:19:58.55 PinPort.in :locking @15:19:58.66 PinPort.seek :locked @15:19:59.18 Archive.in :fast @15:19:59.29 Lysis2.in :fast @15:19:59.36 Lysis1.seek 1100uL @15:19:59.38 Archive.seek :full @15:19:59.59 Lysis2.seek 1100uL @15:20:03.94 delayUntil "15:29:51.21" @15:29:51.22 Lysis2.in :DAmix @15:29:51.38 Lysis1.in :DAmix @15:29:51.91 Lysis2.seek 850uL @15:29:51.98 Lysis1.seek 850uL @15:30:26.62 delay 5 seconds @15:30:31.64 Handoff.select SPR @15:30:31.80 SPR.command "start 2 14" @15:30:33.45 SPR stabilized @15:30:33.63 delay 10 seconds @15:30:43.65 SPR.command "start 2 0" @15:30:46.29 Waiting up to 11:00 for SPR to enter state 1 @15:32:43.97 SPR.command "hold_on" @15:32:45.35 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @15:32:46.01 Lysis2.seek 550uL @15:32:46.07 Lysis1.seek 550uL @15:33:27.60 delay 15 seconds @15:33:42.62 SPR.command "hold_off" @15:33:46.00 Waiting up to 1:00:00 for SPR to enter state 7 @15:35:57.09 Session Begun @15:35:59.66 -> showlog @15:43:22.61 -> showlog -300 @15:50:01.57 SPRsummary:0.0827RIU,953RIU,174RIU,110RIU after 1913s @15:56:15.46 Session Begun @16:01:28.36 delay 10 seconds @16:01:38.39 SPR.command "start 2 0" @16:01:41.07 Waiting up to 11:00 for SPR to enter state 1 @16:03:38.75 SPR.command "hold_on" @16:03:40.14 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:03:40.79 Lysis2.seek 400uL @16:03:40.87 Lysis1.seek 400uL @16:04:01.77 delay 15 seconds @16:04:16.79 SPR.command "hold_off" @16:04:19.98 Waiting up to 1:00:00 for SPR to enter state 7 @16:32:02.33 delay 10 seconds @16:32:12.36 SPR.command "start 2 0" @16:32:15.05 Waiting up to 11:00 for SPR to enter state 1 @16:34:12.71 SPR.command "hold_on" @16:34:14.10 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @16:34:14.84 Lysis2.seek 250uL @16:34:14.93 Lysis1.seek 250uL @16:34:35.82 delay 15 seconds @16:34:50.85 SPR.command "hold_off" @16:34:54.48 Waiting up to 1:00:00 for SPR to enter state 7 @17:02:37.26 delay 10 seconds @17:02:47.29 SPR.command "start 2 0" @17:02:49.97 Waiting up to 11:00 for SPR to enter state 1 @17:04:47.64 SPR.command "hold_on" @17:04:49.02 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @17:04:49.72 Lysis2.seek 100uL @17:04:49.80 Lysis1.seek 100uL @17:05:10.72 delay 15 seconds @17:05:25.75 SPR.command "hold_off" @17:05:29.39 Waiting up to 1:00:00 for SPR to enter state 7 @17:19:00.55 Session Ended @17:33:12.03 SPR analysis completed @17:33:19.35 Ended log spr24jun1115h.spr.bz2 @17:33:19.37 SPR.command "stats_off" @17:33:20.19 Handoff.select :close @17:33:20.38
Gate.power :heat,:OFF @17:33:20.41 Gate.power :cartridge,:OFF @17:33:20.58 PinPort.in :unlocking @17:33:20.67 PinPort.seek :clear @17:33:21.17 Carousel.in :default @17:33:21.33 Carousel.home.seek 50 @17:33:21.69 Plunge.seek :locked @17:33:21.90 Archive.in :unlocking @17:33:22.07 Archive.seek :clear @17:33:22.22 Air.in :unlocking @17:33:22.37 Air.seek :clear @17:33:22.51 Lysis1.in :unlocking @17:33:22.73 Lysis1.seek :clear @17:33:22.85 Lysis2.in :unlocking @17:33:22.97 Lysis2.seek :clear @17:33:27.42
Gate.power :analytic1,:OFF @17:33:27.44 Gate.power :motor,:OFF @17:33:27.50 Gate.power :core,:OFF @17:33:27.54 Gate.power :main,:OFF @17:33:27.59 Safely stopped and ready to power off @17:33:28.34 Gate.power :sampler,:OFF,limit to 3500mA @17:33:28.36 Gate.power :skimmer,:OFF,limit to 3500mA @17:33:31.35 Gate.power :main,:ON,limit to 5000mA @17:33:31.37 Gate.power :analytic1,:ON,limit to 4000mA @17:33:31.51 Powering core bus @17:33:33.82 SPR Module 1, FW Rev. B, FPGA Rev. C @17:33:34.28
Plunge.configure :default @17:33:34.40 SamplePump.configure :default @17:33:34.51 Toroid.configure :default @17:33:34.66 Carousel.configure :default @17:33:34.77 Abs.configure :default @17:33:34.89 PhaseSeparator.configure :default @17:33:35.00 Archive.configure :default @17:33:35.12 Air.configure :default @17:33:35.21 Lysis1.configure :default @17:33:35.81 Lysis2.configure :default @17:33:35.95 PinPort.configure :default @17:33:36.03 All elf controllers running firmware version 4.92 @17:33:36.06 Gate.power :motor,:ON,limit to 5000mA @17:33:36.39 Plunge.home.jog 0 @17:33:37.18 Lysis2.home.jog 0 @17:33:37.21 Air.home.jog 0 @17:33:37.26 Carousel.home.jog 0 @17:33:37.35 Lysis1.home.jog 0 @17:33:37.40 Archive.home.jog 0 @17:33:37.47 Toroid.home.jog 0 @17:33:37.51 PinPort.home.jog 0 @17:33:38.45 Plunge.in :homing @17:33:38.72 Plunge.jog 1604 @17:33:38.82 Lysis2.in :homing @17:33:38.89 Air.in :homing @17:33:38.94 Toroid.in :homing @17:33:38.99 Lysis1.in :homing @17:33:39.06 Archive.in :homing @17:33:39.11 PinPort.in :homing @17:33:39.46 Lysis2.jog 1409 @17:33:39.53 Air.jog 1368 @17:33:39.65 Toroid.jog 636 @17:33:39.78 Lysis1.jog 1397 @17:33:39.81 PinPort.jog -105 @17:33:39.90 Archive.jog 1365 @17:33:40.35 PinPort.setRawPosition! 0 @17:33:40.45 PinPort.seek :home @17:33:40.63 Plunge.setRawPosition! 50 @17:33:40.66 PinPort.in :default @17:33:40.81 Plunge.seek :home @17:33:40.97 Lysis2.setRawPosition! 50 @17:33:41.07 Lysis2.seek :home @17:33:41.23 Plunge.in :default @17:33:41.27 Air.setRawPosition! 50 @17:33:41.46 Air.seek :home @17:33:41.63 Lysis2.in :default @17:33:41.65 Lysis1.setRawPosition! 50 @17:33:41.71 Archive.setRawPosition! 50 @17:33:41.93 Lysis1.seek :home @17:33:41.96 Archive.seek :home @17:33:42.18 Air.in :default @17:33:42.35 Lysis1.in :default @17:33:42.37 Toroid.setRawPosition! 0 @17:33:42.57 Toroid.seek :home @17:33:42.70 Archive.in :default @17:33:42.84 Toroid.in :default @17:33:43.08 Toroid.seek :clear @17:33:44.82
Carousel.in :default @17:33:44.98 Carousel.home.seek 50 @17:33:45.27 Selecting Cartridge 49 @17:33:45.60 Carousel.seek 49 @17:33:46.28 Lysis1.in :fast @17:33:46.38 Lysis1.seek :locked @17:33:49.10 Plunge.seek :locked @17:33:49.29 Air.in :fast @17:33:49.39 Air.seek :locked @17:33:50.40
Carousel.reconfigure :locked @17:33:50.63 Powering cartridge bus @17:33:53.29 Gate.setIR 1750,5000,0,6 @17:33:53.41 Gate.setIR 0,5000,0,6 @17:33:53.52 PuckHeater.configure :default @17:33:53.63 Gate.power :heat,:ON,limit to 5000mA @17:33:54.25 Mounted lysis Cartridge @17:33:54.30 WARNING: Using default puck temperature sensor calibration @17:33:54.34 Using default cartridge heat loss curve for HeatingAccelInCarousel @17:33:56.00 SPR.command "read_sequences" @17:33:56.40 SPR.command "stats_on" @17:33:56.62 SPR.command "start 0 1" @17:33:57.30 SPR.start /var/log/methane/spr24jun1117h.spr.bz2 @17:33:59.25 Waiting for baseline SPR stabilization @17:33:59.28 Handoff.select :waste @17:33:59.49 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @17:34:06.11 Syringe tare is 52% of full scale at 22C (drifted 1%) @17:34:06.15 PinPort.in :locking @17:34:06.27 PinPort.seek :locked @17:34:06.83 Archive.in :fast @17:34:06.95 Lysis2.in :fast @17:34:07.01 Lysis1.seek 1100uL @17:34:07.11 Archive.seek :full @17:34:07.24 Lysis2.seek 1100uL @17:34:11.66 delayUntil "17:43:58.82" @17:43:58.84 Lysis2.in :DAmix @17:43:59.00 Lysis1.in :DAmix @17:43:59.61 Lysis2.seek 850uL @17:43:59.68 Lysis1.seek 850uL @17:44:34.40 delay 5 seconds @17:44:39.43 Handoff.select SPR @17:44:39.60 SPR.command "start 2 14" @17:44:41.05 SPR stabilized @17:44:41.25 delay 10 seconds @17:44:51.27 SPR.command "start 2 0" @17:44:53.90 Waiting up to 11:00 for SPR to enter state 1 @17:46:51.57 SPR.command "hold_on" @17:46:53.48 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @17:46:54.12 Lysis2.seek 550uL @17:46:54.18 Lysis1.seek 550uL @17:47:35.75 delay 15 seconds @17:47:50.78 SPR.command "hold_off" @17:47:54.13 Waiting up to 1:00:00 for SPR to enter state 7 @18:04:09.18 SPRsummary:1.45RIU,172RIU,213RIU,204RIU after 1913s @18:15:35.97 delay 10 seconds @18:15:45.99 SPR.command "start 2 0" @18:15:48.68 Waiting up to 11:00 for SPR to enter state 1 @18:17:46.35 SPR.command "hold_on" @18:17:47.73 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:17:48.34 Lysis2.seek 400uL @18:17:48.38 Lysis1.seek 400uL @18:18:09.33 delay 15 seconds @18:18:24.35 SPR.command "hold_off" @18:18:27.58 Waiting up to 1:00:00 for SPR to enter state 7 @18:19:48.02 Session Ended @18:46:09.93 delay 10 seconds @18:46:19.96 SPR.command "start 2 0" @18:46:22.65 Waiting up to 11:00 for SPR to enter state 1 @18:48:20.32 SPR.command "hold_on" @18:48:21.71 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @18:48:22.39 Lysis2.seek 250uL @18:48:22.49 Lysis1.seek 250uL @18:48:43.36 delay 15 seconds @18:48:58.38 SPR.command "hold_off" @18:49:01.55 Waiting up to 1:00:00 for SPR to enter state 7 @19:16:43.91 delay 10 seconds @19:16:53.94 SPR.command "start 2 0" @19:16:57.15 Waiting up to 11:00 for SPR to enter state 1 @19:18:55.23 SPR.command "hold_on" @19:18:56.62 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @19:18:57.30 Lysis2.seek 100uL @19:18:57.36 Lysis1.seek 100uL @19:19:18.26 delay 15 seconds @19:19:33.28 SPR.command "hold_off" @19:19:36.47 Waiting up to 1:00:00 for SPR to enter state 7 @19:47:18.69 SPR analysis completed @19:47:25.94 Ended log spr24jun1117h.spr.bz2 @19:47:25.96 SPR.command "stats_off" @19:47:26.86 Handoff.select :close @19:47:27.04
Gate.power :heat,:OFF @19:47:27.06 Gate.power :cartridge,:OFF @19:47:27.18 PinPort.in :unlocking @19:47:27.26 PinPort.seek :clear @19:47:27.75 Carousel.in :default @19:47:27.90 Carousel.home.seek 49 @19:47:28.24 Plunge.seek :locked @19:47:28.42 Archive.in :unlocking @19:47:28.60 Archive.seek :clear @19:47:28.72 Air.in :unlocking @19:47:28.86 Air.seek :clear @19:47:28.99 Lysis1.in :unlocking @19:47:29.16 Lysis1.seek :clear @19:47:29.73 Lysis2.in :unlocking @19:47:29.84 Lysis2.seek :clear @19:47:34.28
Gate.power :analytic1,:OFF @19:47:34.32 Gate.power :motor,:OFF @19:47:34.34 Gate.power :core,:OFF @19:47:34.37 Gate.power :main,:OFF @19:47:34.41 Safely stopped and ready to power off @19:47:34.70 Gate.power :sampler,:OFF,limit to 3500mA @19:47:34.74 Gate.power :skimmer,:OFF,limit to 3500mA @19:47:38.18 Gate.power :main,:ON,limit to 5000mA @19:47:38.20 Gate.power :analytic1,:ON,limit to 4000mA @19:47:38.34 Powering core bus @19:47:40.67 SPR Module 1, FW Rev. B, FPGA Rev. C @19:47:41.12
Plunge.configure :default @19:47:41.21 SamplePump.configure :default @19:47:41.32 Toroid.configure :default @19:47:41.43 Carousel.configure :default @19:47:41.54 Abs.configure :default @19:47:41.63 PhaseSeparator.configure :default @19:47:41.74 Archive.configure :default @19:47:41.84 Air.configure :default @19:47:41.91 Lysis1.configure :default @19:47:41.98 Lysis2.configure :default @19:47:42.07 PinPort.configure :default @19:47:42.15 All elf controllers running firmware version 4.92 @19:47:42.18 Gate.power :motor,:ON,limit to 5000mA @19:47:42.62 Plunge.home.jog 0 @19:47:43.26 PinPort.home.jog 0 @19:47:43.31 Lysis2.home.jog 0 @19:47:43.36 Air.home.jog 0 @19:47:43.40 Archive.home.jog 0 @19:47:43.43 Toroid.home.jog 0 @19:47:43.48 Carousel.home.jog 0 @19:47:43.59 Lysis1.home.jog 0 @19:47:44.41 Plunge.in :homing @19:47:44.73 Plunge.jog 1604 @19:47:44.92 Air.in :homing @19:47:45.04 Lysis2.in :homing @19:47:45.07 Toroid.in :homing @19:47:45.11 PinPort.in :homing @19:47:45.14 Lysis1.in :homing @19:47:45.19 Archive.in :homing @19:47:45.48 Air.jog 1368 @19:47:45.52 Lysis2.jog 1405 @19:47:45.60 Toroid.jog 634 @19:47:45.67 Lysis1.jog 1397 @19:47:45.73 PinPort.jog -106 @19:47:45.82 Archive.jog 1367 @19:47:46.29 Plunge.setRawPosition! 50 @19:47:46.32 PinPort.setRawPosition! 0 @19:47:46.47 Plunge.seek :home @19:47:46.51 PinPort.seek :home @19:47:46.78 PinPort.in :default @19:47:46.92 Plunge.in :default @19:47:47.03 Air.setRawPosition! 50 @19:47:47.11 Air.seek :home @19:47:47.26 Lysis1.setRawPosition! 50 @19:47:47.29 Archive.setRawPosition! 50 @19:47:47.33 Lysis2.setRawPosition! 50 @19:47:47.50 Lysis1.seek :home @19:47:47.56 Lysis2.seek :home @19:47:48.05 Archive.seek :home @19:47:48.44 Lysis1.in :default @19:47:48.48 Air.in :default @19:47:48.52 Toroid.setRawPosition! 0 @19:47:48.75 Toroid.seek :home @19:47:48.90 Archive.in :default @19:47:48.95 Toroid.in :default @19:47:48.99 Lysis2.in :default @19:47:49.45 Toroid.seek :clear @19:47:51.17
Carousel.in :default @19:47:51.33 Carousel.home.seek 49 @19:47:51.66 Selecting Cartridge 48 @19:47:51.97 Carousel.seek 48 @19:47:52.52 Lysis1.in :fast @19:47:52.63 Lysis1.seek :locked @19:47:55.27 Plunge.seek :locked @19:47:55.45 Air.in :fast @19:47:55.53 Air.seek :locked @19:47:56.53
Carousel.reconfigure :locked @19:47:56.74 Powering cartridge bus @19:47:59.39 Gate.setIR 1750,5000,0,6 @19:47:59.52 Gate.setIR 0,5000,0,6 @19:47:59.61 PuckHeater.configure :default @19:47:59.70 Gate.power :heat,:ON,limit to 5000mA @19:48:00.33 Mounted lysis Cartridge @19:48:00.36 WARNING: Using default puck temperature sensor calibration @19:48:00.41 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:48:02.03 SPR.command "read_sequences" @19:48:02.29 SPR.command "stats_on" @19:48:02.46 SPR.command "start 0 1" @19:48:03.17 SPR.start /var/log/methane/spr24jun1119h.spr.bz2 @19:48:05.66 Waiting for baseline SPR stabilization @19:48:05.68 Handoff.select :waste @19:48:05.86 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:48:12.50 Syringe tare is 52% of full scale at 21C (drifted 1%) @19:48:12.53 PinPort.in :locking @19:48:12.67 PinPort.seek :locked @19:48:13.22 Archive.in :fast @19:48:13.29 Lysis2.in :fast @19:48:13.37 Lysis1.seek 1100uL @19:48:13.53 Archive.seek :full @19:48:13.60 Lysis2.seek 1100uL @19:48:17.93 delayUntil "19:58:05.63" @19:58:06.01 Lysis2.in :DAmix @19:58:06.26 Lysis1.in :DAmix @19:58:06.81 Lysis2.seek 850uL @19:58:06.92 Lysis1.seek 850uL @19:58:41.51 delay 5 seconds @19:58:46.53 Handoff.select SPR @19:58:46.72 SPR.command "start 2 14" @19:58:47.88 SPR stabilized @19:58:48.06 delay 10 seconds @19:58:58.08 SPR.command "start 2 0" @19:59:00.73 Waiting up to 11:00 for SPR to enter state 1 @20:00:58.40 SPR.command "hold_on" @20:00:59.78 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @20:01:00.42 Lysis2.seek 550uL @20:01:00.48 Lysis1.seek 550uL @20:01:42.05 delay 15 seconds @20:01:57.07 SPR.command "hold_off" @20:02:00.96 Waiting up to 1:00:00 for SPR to enter state 7 @20:18:16.95 SPRsummary:3.79e+09RIU,79.9RIU,170RIU,189RIU after 1915s @20:29:43.75 delay 10 seconds @20:29:53.77 SPR.command "start 2 0" @20:29:56.45 Waiting up to 11:00 for SPR to enter state 1 @20:31:54.13 SPR.command "hold_on" @20:31:56.03 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:31:56.63 Lysis2.seek 400uL @20:31:56.68 Lysis1.seek 400uL @20:32:17.57 delay 15 seconds @20:32:32.59 SPR.command "hold_off" @20:32:35.88 Waiting up to 1:00:00 for SPR to enter state 7 @21:00:17.71 delay 10 seconds @21:00:27.73 SPR.command "start 2 0" @21:00:30.95 Waiting up to 11:00 for SPR to enter state 1 @21:02:29.04 SPR.command "hold_on" @21:02:30.43 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:02:31.20 Lysis2.seek 250uL @21:02:31.27 Lysis1.seek 250uL @21:02:52.11 delay 15 seconds @21:03:07.13 SPR.command "hold_off" @21:03:10.79 Waiting up to 1:00:00 for SPR to enter state 7 @21:17:02.22 Session Begun @21:17:06.80 -> showlog @21:17:13.40 -> showlog -300 @21:18:10.60 -> exit @21:18:10.62 Session Ended @21:30:53.57 delay 10 seconds @21:31:03.60 SPR.command "start 2 0" @21:31:07.24 Waiting up to 11:00 for SPR to enter state 1 @21:33:05.32 SPR.command "hold_on" @21:33:06.82 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:33:07.41 Lysis2.seek 100uL @21:33:07.45 Lysis1.seek 100uL @21:33:28.32 delay 15 seconds @21:33:43.34 SPR.command "hold_off" @21:33:46.14 Waiting up to 1:00:00 for SPR to enter state 7 @22:01:27.41 SPR analysis completed @22:01:34.51 Ended log spr24jun1119h.spr.bz2 @22:01:34.53 SPR.command "stats_off" @22:01:35.58 Handoff.select :close @22:01:35.76
Gate.power :heat,:OFF @22:01:35.79 Gate.power :cartridge,:OFF @22:01:35.91 PinPort.in :unlocking @22:01:35.99 PinPort.seek :clear @22:01:36.48 Carousel.in :default @22:01:36.67 Carousel.home.seek 48 @22:01:37.02 Plunge.seek :locked @22:01:37.67 Archive.in :unlocking @22:01:37.82 Archive.seek :clear @22:01:37.95 Air.in :unlocking @22:01:38.11 Air.seek :clear @22:01:38.24 Lysis1.in :unlocking @22:01:38.40 Lysis1.seek :clear @22:01:38.51 Lysis2.in :unlocking @22:01:38.65 Lysis2.seek :clear @22:01:43.08
Gate.power :analytic1,:OFF @22:01:43.12 Gate.power :motor,:OFF @22:01:43.14 Gate.power :core,:OFF @22:01:43.17 Gate.power :main,:OFF @22:01:43.20 Safely stopped and ready to power off @22:01:43.87 Gate.power :sampler,:OFF,limit to 3500mA @22:01:43.90 Gate.power :skimmer,:OFF,limit to 3500mA @22:01:46.98 Gate.power :main,:ON,limit to 5000mA @22:01:47.00 Gate.power :analytic1,:ON,limit to 4000mA @22:01:47.14 Powering core bus @22:01:49.44 SPR Module 1, FW Rev. B, FPGA Rev. C @22:01:49.91
Plunge.configure :default @22:01:50.02 SamplePump.configure :default @22:01:50.13 Toroid.configure :default @22:01:50.22 Carousel.configure :default @22:01:50.32 Abs.configure :default @22:01:50.42 PhaseSeparator.configure :default @22:01:50.53 Archive.configure :default @22:01:50.66 Air.configure :default @22:01:50.75 Lysis1.configure :default @22:01:50.81 Lysis2.configure :default @22:01:50.92 PinPort.configure :default @22:01:51.00 All elf controllers running firmware version 4.92 @22:01:51.03 Gate.power :motor,:ON,limit to 5000mA @22:01:51.22 Plunge.home.jog 0 @22:01:52.07 Lysis2.home.jog 0 @22:01:52.11 Air.home.jog 0 @22:01:52.16 Carousel.home.jog 0 @22:01:52.23 Lysis1.home.jog 0 @22:01:52.27 Archive.home.jog 0 @22:01:52.34 Toroid.home.jog 0 @22:01:52.38 PinPort.home.jog 0 @22:01:53.31 Plunge.in :homing @22:01:53.55 Plunge.jog 1607 @22:01:53.71 PinPort.in :homing @22:01:53.81 Lysis1.in :homing @22:01:53.88 Archive.in :homing @22:01:53.94 Toroid.in :homing @22:01:54.01 Air.in :homing @22:01:54.07 Lysis2.in :homing @22:01:54.15 PinPort.jog -106 @22:01:54.76 Lysis1.jog 1395 @22:01:55.02 Archive.jog 1368 @22:01:55.17 Air.jog 1367 @22:01:55.22 Lysis2.jog 1406 @22:01:55.29 Toroid.jog 626 @22:01:55.65 Plunge.setRawPosition! 50 @22:01:55.68 PinPort.setRawPosition! 0 @22:01:55.82 PinPort.seek :home @22:01:55.85 Plunge.seek :home @22:01:56.10 PinPort.in :default @22:01:56.23 Plunge.in :default @22:01:56.34 Lysis1.setRawPosition! 50 @22:01:56.41 Lysis1.seek :home @22:01:56.53 Archive.setRawPosition! 50 @22:01:56.66 Archive.seek :home @22:01:56.81 Lysis1.in :default @22:01:56.85 Air.setRawPosition! 50 @22:01:56.89 Lysis2.setRawPosition! 50 @22:01:57.09 Lysis2.seek :home @22:01:57.14 Air.seek :home @22:01:57.33 Archive.in :default @22:01:57.45 Lysis2.in :default @22:01:57.57 Air.in :default @22:01:57.67 Toroid.setRawPosition! 0 @22:01:57.75 Toroid.seek :home @22:01:57.88 Toroid.in :default @22:01:58.01 Toroid.seek :clear @22:01:59.71
Carousel.in :default @22:01:59.86 Carousel.home.seek 48 @22:02:00.15 Selecting Cartridge 47 @22:02:00.47 Carousel.seek 47 @22:02:01.02 Lysis1.in :fast @22:02:01.09 Lysis1.seek :locked @22:02:03.74 Plunge.seek :locked @22:02:03.93 Air.in :fast @22:02:04.01 Air.seek :locked @22:02:05.01
Carousel.reconfigure :locked @22:02:05.19 Powering cartridge bus @22:02:07.84 Gate.setIR 1750,5000,0,6 @22:02:07.97 Gate.setIR 0,5000,0,6 @22:02:08.07 PuckHeater.configure :default @22:02:08.15 Gate.power :heat,:ON,limit to 5000mA @22:02:08.77 Mounted lysis Cartridge @22:02:08.82 WARNING: Using default puck temperature sensor calibration @22:02:08.84 Using default cartridge heat loss curve for HeatingAccelInCarousel @22:02:10.92 SPR.command "read_sequences" @22:02:11.09 SPR.command "stats_on" @22:02:11.26 SPR.command "start 0 1" @22:02:11.97 SPR.start /var/log/methane/spr24jun1122h.spr.bz2 @22:02:13.95 Waiting for baseline SPR stabilization @22:02:13.97 Handoff.select :waste @22:02:14.14 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @22:02:20.77 Syringe tare is 52% of full scale at 21C (drifted 1%) @22:02:20.82 PinPort.in :locking @22:02:20.91 PinPort.seek :locked @22:02:21.43 Archive.in :fast @22:02:21.50 Lysis2.in :fast @22:02:21.59 Lysis1.seek 1100uL @22:02:21.75 Archive.seek :full @22:02:21.87 Lysis2.seek 1100uL @22:02:26.16 delayUntil "22:12:13.52" @22:12:13.53 Lysis2.in :DAmix @22:12:13.67 Lysis1.in :DAmix @22:12:14.23 Lysis2.seek 850uL @22:12:14.31 Lysis1.seek 850uL @22:12:48.93 delay 5 seconds @22:12:53.96 Handoff.select SPR @22:12:54.12 SPR.command "start 2 14" @22:12:55.74 SPR stabilized @22:12:55.91 delay 10 seconds @22:13:05.93 SPR.command "start 2 0" @22:13:08.60 Waiting up to 11:00 for SPR to enter state 1 @22:15:06.26 SPR.command "hold_on" @22:15:07.64 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @22:15:08.28 Lysis2.seek 550uL @22:15:08.34 Lysis1.seek 550uL @22:15:49.88 delay 15 seconds @22:16:04.91 SPR.command "hold_off" @22:16:08.29 Waiting up to 1:00:00 for SPR to enter state 7 @22:32:23.86 SPRsummary:3.95e+09RIU,96.6RIU,89.8RIU,126RIU after 1913s @22:43:50.67 delay 10 seconds @22:44:00.70 SPR.command "start 2 0" @22:44:03.36 Waiting up to 11:00 for SPR to enter state 1 @22:46:01.04 SPR.command "hold_on" @22:46:02.42 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @22:46:03.51 Lysis2.seek 400uL @22:46:03.62 Lysis1.seek 400uL @22:46:24.42 delay 15 seconds @22:46:39.44 SPR.command "hold_off" @22:46:42.79 Waiting up to 1:00:00 for SPR to enter state 7 @23:14:25.57 delay 10 seconds @23:14:35.59 SPR.command "start 2 0" @23:14:38.28 Waiting up to 11:00 for SPR to enter state 1 @23:16:35.94 SPR.command "hold_on" @23:16:37.86 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:16:38.53 Lysis2.seek 250uL @23:16:38.66 Lysis1.seek 250uL @23:16:59.51 delay 15 seconds @23:17:14.53 SPR.command "hold_off" @23:17:17.18 Waiting up to 1:00:00 for SPR to enter state 7 @23:44:58.64 delay 10 seconds @23:45:08.66 SPR.command "start 2 0" @23:45:11.31 Waiting up to 11:00 for SPR to enter state 1 @23:47:08.98 SPR.command "hold_on" @23:47:10.88 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @23:47:11.56 Lysis2.seek 100uL @23:47:11.60 Lysis1.seek 100uL @23:47:32.51 delay 15 seconds @23:47:47.53 SPR.command "hold_off" @23:47:50.21 Waiting up to 1:00:00 for SPR to enter state 7 @00:15:31.48PDT12-Jun-24 SPR analysis completed @00:15:38.70 Ended log spr24jun1122h.spr.bz2 @00:15:38.72 SPR.command "stats_off" @00:15:39.64 Handoff.select :close @00:15:39.82
Gate.power :heat,:OFF @00:15:39.86 Gate.power :cartridge,:OFF @00:15:39.97 PinPort.in :unlocking @00:15:40.04 PinPort.seek :clear @00:15:40.53 Carousel.in :default @00:15:40.71 Carousel.home.seek 47 @00:15:41.05 Plunge.seek :locked @00:15:41.25 Archive.in :unlocking @00:15:41.41 Archive.seek :clear @00:15:41.52 Air.in :unlocking @00:15:41.68 Air.seek :clear @00:15:41.81 Lysis1.in :unlocking @00:15:42.42 Lysis1.seek :clear @00:15:42.56 Lysis2.in :unlocking @00:15:42.75 Lysis2.seek :clear @00:15:47.19
Gate.power :analytic1,:OFF @00:15:47.22 Gate.power :motor,:OFF @00:15:47.26 Gate.power :core,:OFF @00:15:47.30 Gate.power :main,:OFF @00:15:47.33 Safely stopped and ready to power off @00:15:47.55 Gate.power :sampler,:OFF,limit to 3500mA @00:15:47.58 Gate.power :skimmer,:OFF,limit to 3500mA @00:15:51.10 Gate.power :main,:ON,limit to 5000mA @00:15:51.13 Gate.power :analytic1,:ON,limit to 4000mA @00:15:51.28 Powering core bus @00:15:53.59 SPR Module 1, FW Rev. B, FPGA Rev. C @00:15:54.05
Plunge.configure :default @00:15:54.16 SamplePump.configure :default @00:15:54.26 Toroid.configure :default @00:15:54.37 Carousel.configure :default @00:15:54.47 Abs.configure :default @00:15:54.61 PhaseSeparator.configure :default @00:15:54.71 Archive.configure :default @00:15:54.82 Air.configure :default @00:15:54.90 Lysis1.configure :default @00:15:54.98 Lysis2.configure :default @00:15:55.09 PinPort.configure :default @00:15:55.17 All elf controllers running firmware version 4.92 @00:15:55.20 Gate.power :motor,:ON,limit to 5000mA @00:15:55.60 Plunge.home.jog 0 @00:15:56.24 Lysis2.home.jog 0 @00:15:56.30 Air.home.jog 0 @00:15:56.34 Carousel.home.jog 0 @00:15:56.40 Toroid.home.jog 0 @00:15:56.45 Lysis1.home.jog 0 @00:15:56.50 Archive.home.jog 0 @00:15:56.57 PinPort.home.jog 0 @00:15:57.47 Plunge.in :homing @00:15:57.67 Plunge.jog 1602 @00:15:57.90 PinPort.in :homing @00:15:57.97 Lysis2.in :homing @00:15:58.02 Air.in :homing @00:15:58.07 Toroid.in :homing @00:15:58.11 Lysis1.in :homing @00:15:58.16 Archive.in :homing @00:15:58.41 PinPort.jog -106 @00:15:58.47 Lysis2.jog 1409 @00:15:58.72 Air.jog 1368 @00:15:58.82 Lysis1.jog 1395 @00:15:58.86 Archive.jog 1368 @00:15:58.97 Toroid.jog 626 @00:15:59.30 Plunge.setRawPosition! 50 @00:15:59.33 PinPort.setRawPosition! 0 @00:15:59.92 PinPort.seek :home @00:16:00.03 Plunge.seek :home @00:16:00.33 PinPort.in :default @00:16:00.37 Lysis2.setRawPosition! 50 @00:16:00.41 Air.setRawPosition! 50 @00:16:00.45 Lysis1.setRawPosition! 50 @00:16:00.50 Archive.setRawPosition! 50 @00:16:00.82 Lysis1.seek :home @00:16:00.87 Archive.seek :home @00:16:00.94 Air.seek :home @00:16:00.98 Lysis2.seek :home @00:16:01.44 Lysis1.in :default @00:16:01.49 Archive.in :default @00:16:01.53 Plunge.in :default @00:16:01.56 Toroid.setRawPosition! 0 @00:16:01.79 Toroid.seek :home @00:16:01.96 Lysis2.in :default @00:16:02.00 Air.in :default @00:16:02.08 Toroid.in :default @00:16:02.49 Toroid.seek :clear @00:16:04.24
Carousel.in :default @00:16:04.39 Carousel.home.seek 47 @00:16:04.68 Selecting Cartridge 46 @00:16:04.99 Carousel.seek 46 @00:16:05.54 Lysis1.in :fast @00:16:05.63 Lysis1.seek :locked @00:16:08.27 Plunge.seek :locked @00:16:08.46 Air.in :fast @00:16:08.55 Air.seek :locked @00:16:09.57
Carousel.reconfigure :locked @00:16:09.74 Powering cartridge bus @00:16:12.41 Gate.setIR 1750,5000,0,6 @00:16:12.55 Gate.setIR 0,5000,0,6 @00:16:12.66 PuckHeater.configure :default @00:16:12.74 Gate.power :heat,:ON,limit to 5000mA @00:16:13.36 Mounted lysis Cartridge @00:16:13.40 WARNING: Using default puck temperature sensor calibration @00:16:13.44 Using default cartridge heat loss curve for HeatingAccelInCarousel @00:16:14.99 SPR.command "read_sequences" @00:16:15.22 SPR.command "stats_on" @00:16:15.39 SPR.command "start 0 1" @00:16:16.11 SPR.start /var/log/methane/spr24jun1200h.spr.bz2 @00:16:18.07 Waiting for baseline SPR stabilization @00:16:18.10 Handoff.select :waste @00:16:18.29 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @00:16:24.92 Syringe tare is 52% of full scale at 21C (drifted 1%) @00:16:24.95 PinPort.in :locking @00:16:25.05 PinPort.seek :locked @00:16:25.56 Archive.in :fast @00:16:25.62 Lysis2.in :fast @00:16:25.76 Lysis1.seek 1100uL @00:16:25.83 Archive.seek :full @00:16:25.90 Lysis2.seek 1100uL @00:16:30.27 delayUntil "00:26:17.65" @00:26:17.67 Lysis2.in :DAmix @00:26:17.82 Lysis1.in :DAmix @00:26:18.41 Lysis2.seek 850uL @00:26:18.44 Lysis1.seek 850uL @00:26:53.15 delay 5 seconds @00:26:58.17 Handoff.select SPR @00:26:58.33 SPR.command "start 2 14" @00:26:59.35 SPR stabilized @00:26:59.52 delay 10 seconds @00:27:09.55 SPR.command "start 2 0" @00:27:13.24 Waiting up to 11:00 for SPR to enter state 1 @00:29:11.34 SPR.command "hold_on" @00:29:12.72 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @00:29:13.38 Lysis2.seek 550uL @00:29:13.44 Lysis1.seek 550uL @00:29:54.98 delay 15 seconds @00:30:10.01 SPR.command "hold_off" @00:30:13.37 Waiting up to 1:00:00 for SPR to enter state 7 @00:46:28.94 SPRsummary:-10.8RIU,64.8RIU,53.5RIU,66.9RIU after 1914s @00:57:55.74 delay 10 seconds @00:58:05.76 SPR.command "start 2 0" @00:58:08.44 Waiting up to 11:00 for SPR to enter state 1 @01:00:06.12 SPR.command "hold_on" @01:00:07.50 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:00:08.12 Lysis2.seek 400uL @01:00:08.22 Lysis1.seek 400uL @01:00:29.06 delay 15 seconds @01:00:44.08 SPR.command "hold_off" @01:00:47.34 Waiting up to 1:00:00 for SPR to enter state 7 @01:28:29.71 delay 10 seconds @01:28:39.73 SPR.command "start 2 0" @01:28:42.41 Waiting up to 11:00 for SPR to enter state 1 @01:30:40.09 SPR.command "hold_on" @01:30:41.47 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @01:30:42.16 Lysis2.seek 250uL @01:30:42.23 Lysis1.seek 250uL @01:31:03.10 delay 15 seconds @01:31:18.12 SPR.command "hold_off" @01:31:21.31 Waiting up to 1:00:00 for SPR to enter state 7 @01:59:03.67 delay 10 seconds @01:59:13.69 SPR.command "start 2 0" @01:59:16.91 Waiting up to 11:00 for SPR to enter state 1 @02:01:15.01 SPR.command "hold_on" @02:01:16.91 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @02:01:17.61 Lysis2.seek 100uL @02:01:17.66 Lysis1.seek 100uL @02:01:38.60 delay 15 seconds @02:01:53.64 SPR.command "hold_off" @02:01:56.23 Waiting up to 1:00:00 for SPR to enter state 7 @02:29:37.51 SPR analysis completed @02:29:44.78 Ended log spr24jun1200h.spr.bz2 @02:29:44.80 SPR.command "stats_off" @02:29:45.67 Handoff.select :close @02:29:45.85
Gate.power :heat,:OFF @02:29:45.87 Gate.power :cartridge,:OFF @02:29:45.99 PinPort.in :unlocking @02:29:46.07 PinPort.seek :clear @02:29:46.60 Carousel.in :default @02:29:46.74 Carousel.home.seek 46 @02:29:47.08 Plunge.seek :locked @02:29:47.74 Archive.in :unlocking @02:29:47.89 Archive.seek :clear @02:29:48.02 Air.in :unlocking @02:29:48.15 Air.seek :clear @02:29:48.29 Lysis1.in :unlocking @02:29:48.43 Lysis1.seek :clear @02:29:48.61 Lysis2.in :unlocking @02:29:48.69 Lysis2.seek :clear @02:29:53.12
Gate.power :analytic1,:OFF @02:29:53.14 Gate.power :motor,:OFF @02:29:53.18 Gate.power :core,:OFF @02:29:53.22 Gate.power :main,:OFF @02:29:53.25 Safely stopped and ready to power off @02:29:53.47 Gate.power :sampler,:OFF,limit to 3500mA @02:29:53.49 Gate.power :skimmer,:OFF,limit to 3500mA @02:29:57.02 Gate.power :main,:ON,limit to 5000mA @02:29:57.04 Gate.power :analytic1,:ON,limit to 4000mA @02:29:57.19 Powering core bus @02:29:59.51 SPR Module 1, FW Rev. B, FPGA Rev. C @02:29:59.96
Plunge.configure :default @02:30:00.07 SamplePump.configure :default @02:30:00.18 Toroid.configure :default @02:30:00.29 Carousel.configure :default @02:30:00.39 Abs.configure :default @02:30:00.50 PhaseSeparator.configure :default @02:30:00.64 Archive.configure :default @02:30:00.73 Air.configure :default @02:30:00.81 Lysis1.configure :default @02:30:00.89 Lysis2.configure :default @02:30:01.00 PinPort.configure :default @02:30:01.08 All elf controllers running firmware version 4.92 @02:30:01.11 Gate.power :motor,:ON,limit to 5000mA @02:30:01.36 Plunge.home.jog 0 @02:30:02.14 PinPort.home.jog 0 @02:30:02.17 Lysis1.home.jog 0 @02:30:02.21 Archive.home.jog 0 @02:30:02.26 Toroid.home.jog 0 @02:30:02.33 Lysis2.home.jog 0 @02:30:02.39 Air.home.jog 0 @02:30:02.43 Carousel.home.jog 0 @02:30:03.22 Plunge.in :homing @02:30:03.55 Plunge.jog 1605 @02:30:03.77 Lysis2.in :homing @02:30:03.84 Lysis1.in :homing @02:30:03.89 Archive.in :homing @02:30:03.94 PinPort.in :homing @02:30:03.98 Air.in :homing @02:30:04.03 Toroid.in :homing @02:30:04.76 Lysis2.jog 1406 @02:30:04.88 Lysis1.jog 1398 @02:30:05.09 Archive.jog 1367 @02:30:05.17 PinPort.jog -106 @02:30:05.24 Air.jog 1367 @02:30:05.29 Toroid.jog 631 @02:30:05.64 Plunge.setRawPosition! 50 @02:30:05.72 Plunge.seek :home @02:30:05.86 PinPort.setRawPosition! 0 @02:30:05.94 PinPort.seek :home @02:30:06.07 Plunge.in :default @02:30:06.10 PinPort.in :default @02:30:06.29 Lysis2.setRawPosition! 50 @02:30:06.32 Lysis1.setRawPosition! 50 @02:30:06.48 Lysis2.seek :home @02:30:06.51 Lysis1.seek :home @02:30:06.79 Archive.setRawPosition! 50 @02:30:06.82 Air.setRawPosition! 50 @02:30:06.98 Air.seek :home @02:30:07.01 Archive.seek :home @02:30:07.26 Lysis1.in :default @02:30:07.29 Lysis2.in :default @02:30:07.48 Archive.in :default @02:30:07.51 Air.in :default @02:30:07.69 Toroid.setRawPosition! 0 @02:30:07.77 Toroid.seek :home @02:30:07.92 Toroid.in :default @02:30:08.04 Toroid.seek :clear @02:30:09.72
Carousel.in :default @02:30:09.87 Carousel.home.seek 46 @02:30:10.15 Selecting Cartridge 45 @02:30:10.46 Carousel.seek 45 @02:30:11.00 Lysis1.in :fast @02:30:11.08 Lysis1.seek :locked @02:30:13.73 Plunge.seek :locked @02:30:13.92 Air.in :fast @02:30:13.99 Air.seek :locked @02:30:14.99
Carousel.reconfigure :locked @02:30:15.16 Powering cartridge bus @02:30:17.82 Gate.setIR 1750,5000,0,6 @02:30:17.95 Gate.setIR 0,5000,0,6 @02:30:18.03 PuckHeater.configure :default @02:30:18.12 Gate.power :heat,:ON,limit to 5000mA @02:30:18.74 Mounted lysis Cartridge @02:30:18.80 WARNING: Using default puck temperature sensor calibration @02:30:19.27 Using default cartridge heat loss curve for HeatingAccelInCarousel @02:30:20.92 SPR.command "read_sequences" @02:30:21.14 SPR.command "stats_on" @02:30:21.32 SPR.command "start 0 1" @02:30:22.03 SPR.start /var/log/methane/spr24jun1202h.spr.bz2 @02:30:24.00 Waiting for baseline SPR stabilization @02:30:24.02 Handoff.select :waste @02:30:24.21 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @02:30:30.84 Syringe tare is 52% of full scale at 22C (drifted 1%) @02:30:30.89 PinPort.in :locking @02:30:30.98 PinPort.seek :locked @02:30:31.49 Archive.in :fast @02:30:31.59 Lysis2.in :fast @02:30:31.70 Archive.seek :full @02:30:31.74 Lysis1.seek 1100uL @02:30:31.89 Lysis2.seek 1100uL @02:30:36.23 delayUntil "02:40:23.56" @02:40:23.58 Lysis2.in :DAmix @02:40:23.73 Lysis1.in :DAmix @02:40:24.29 Lysis2.seek 850uL @02:40:24.34 Lysis1.seek 850uL @02:40:59.02 delay 5 seconds @02:41:04.04 Handoff.select SPR @02:41:04.19 SPR.command "start 2 14" @02:41:05.27 SPR stabilized @02:41:05.44 delay 10 seconds @02:41:15.46 SPR.command "start 2 0" @02:41:18.64 Waiting up to 11:00 for SPR to enter state 1 @02:43:16.31 SPR.command "hold_on" @02:43:17.69 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @02:43:18.36 Lysis2.seek 550uL @02:43:18.47 Lysis1.seek 550uL @02:44:00.02 delay 15 seconds @02:44:15.05 SPR.command "hold_off" @02:44:18.35 Waiting up to 1:00:00 for SPR to enter state 7 @03:00:33.91 SPRsummary:3.89e+09RIU,54.5RIU,63.8RIU,2.67e+09RIU after 1913s @03:12:00.76 delay 10 seconds @03:12:10.79 SPR.command "start 2 0" @03:12:13.94 Waiting up to 11:00 for SPR to enter state 1 @03:14:12.04 SPR.command "hold_on" @03:14:13.42 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @03:14:14.02 Lysis2.seek 400uL @03:14:14.08 Lysis1.seek 400uL @03:14:34.98 delay 15 seconds @03:14:50.00 SPR.command "hold_off" @03:14:53.26 Waiting up to 1:00:00 for SPR to enter state 7 @03:42:35.61 delay 10 seconds @03:42:45.63 SPR.command "start 2 0" @03:42:48.34 Waiting up to 11:00 for SPR to enter state 1 @03:44:46.01 SPR.command "hold_on" @03:44:47.39 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @03:44:48.08 Lysis2.seek 250uL @03:44:48.14 Lysis1.seek 250uL @03:45:09.00 delay 15 seconds @03:45:24.02 SPR.command "hold_off" @03:45:27.23 Waiting up to 1:00:00 for SPR to enter state 7 @04:13:09.61 delay 10 seconds @04:13:19.63 SPR.command "start 2 0" @04:13:22.31 Waiting up to 11:00 for SPR to enter state 1 @04:15:19.97 SPR.command "hold_on" @04:15:21.88 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @04:15:22.61 Lysis2.seek 100uL @04:15:22.65 Lysis1.seek 100uL @04:15:43.55 delay 15 seconds @04:15:58.57 SPR.command "hold_off" @04:16:01.73 Waiting up to 1:00:00 for SPR to enter state 7 @04:43:43.43 SPR analysis completed @04:43:50.70 Ended log spr24jun1202h.spr.bz2 @04:43:50.72 SPR.command "stats_off" @04:43:51.60 Handoff.select :close @04:43:51.78
Gate.power :heat,:OFF @04:43:51.82 Gate.power :cartridge,:OFF @04:43:51.92 PinPort.in :unlocking @04:43:52.00 PinPort.seek :clear @04:43:52.49 Carousel.in :default @04:43:52.67 Carousel.home.seek 45 @04:43:53.01 Plunge.seek :locked @04:43:53.21 Archive.in :unlocking @04:43:53.82 Archive.seek :clear @04:43:53.98 Air.in :unlocking @04:43:54.13 Air.seek :clear @04:43:54.27 Lysis1.in :unlocking @04:43:54.42 Lysis1.seek :clear @04:43:54.51 Lysis2.in :unlocking @04:43:54.69 Lysis2.seek :clear @04:43:59.14
Gate.power :analytic1,:OFF @04:43:59.16 Gate.power :motor,:OFF @04:43:59.20 Gate.power :core,:OFF @04:43:59.24 Gate.power :main,:OFF @04:43:59.27 Safely stopped and ready to power off @04:43:59.52 Gate.power :sampler,:OFF,limit to 3500mA @04:43:59.54 Gate.power :skimmer,:OFF,limit to 3500mA @04:44:03.04 Gate.power :main,:ON,limit to 5000mA @04:44:03.07 Gate.power :analytic1,:ON,limit to 4000mA @04:44:03.21 Powering core bus @04:44:05.52 SPR Module 1, FW Rev. B, FPGA Rev. C @04:44:05.99
Plunge.configure :default @04:44:06.10 SamplePump.configure :default @04:44:06.19 Toroid.configure :default @04:44:06.30 Carousel.configure :default @04:44:06.40 Abs.configure :default @04:44:06.51 PhaseSeparator.configure :default @04:44:06.65 Archive.configure :default @04:44:06.76 Air.configure :default @04:44:06.85 Lysis1.configure :default @04:44:06.92 Lysis2.configure :default @04:44:07.02 PinPort.configure :default @04:44:07.10 All elf controllers running firmware version 4.92 @04:44:07.13 Gate.power :motor,:ON,limit to 5000mA @04:44:07.44 Plunge.home.jog 0 @04:44:08.16 PinPort.home.jog 0 @04:44:08.22 Lysis1.home.jog 0 @04:44:08.27 Archive.home.jog 0 @04:44:08.32 Toroid.home.jog 0 @04:44:08.36 Lysis2.home.jog 0 @04:44:08.41 Air.home.jog 0 @04:44:08.46 Carousel.home.jog 0 @04:44:09.31 Plunge.in :homing @04:44:09.70 Plunge.jog 1607 @04:44:09.78 PinPort.in :homing @04:44:09.83 Lysis1.in :homing @04:44:09.88 Archive.in :homing @04:44:09.92 Lysis2.in :homing @04:44:09.97 Air.in :homing @04:44:10.01 Toroid.in :homing @04:44:10.32 PinPort.jog -106 @04:44:10.89 Lysis1.jog 1399 @04:44:11.02 Archive.jog 1370 @04:44:11.07 Lysis2.jog 1407 @04:44:11.14 Toroid.jog 625 @04:44:11.21 Air.jog 1368 @04:44:11.72 Plunge.setRawPosition! 50 @04:44:11.75 PinPort.setRawPosition! 0 @04:44:11.89 Plunge.seek :home @04:44:11.93 PinPort.seek :home @04:44:12.15 PinPort.in :default @04:44:12.27 Plunge.in :default @04:44:12.45 Lysis1.setRawPosition! 50 @04:44:12.55 Lysis1.seek :home @04:44:12.72 Air.setRawPosition! 50 @04:44:12.76 Archive.setRawPosition! 50 @04:44:12.80 Lysis2.setRawPosition! 50 @04:44:13.01 Air.seek :home @04:44:13.09 Lysis2.seek :home @04:44:13.12 Archive.seek :home @04:44:13.41 Lysis1.in :default @04:44:13.57 Lysis2.in :default @04:44:13.60 Air.in :default @04:44:13.64 Archive.in :default @04:44:13.86 Toroid.setRawPosition! 0 @04:44:13.94 Toroid.seek :home @04:44:14.07 Toroid.in :default @04:44:14.21 Toroid.seek :clear @04:44:15.91
Carousel.in :default @04:44:16.05 Carousel.home.seek 45 @04:44:16.33 Selecting Cartridge 44 @04:44:16.67 Carousel.seek 44 @04:44:17.22 Lysis1.in :fast @04:44:17.31 Lysis1.seek :locked @04:44:19.95 Plunge.seek :locked @04:44:20.15 Air.in :fast @04:44:20.22 Air.seek :locked @04:44:21.22
Carousel.reconfigure :locked @04:44:21.39 Powering cartridge bus @04:44:24.05 Gate.setIR 1750,5000,0,6 @04:44:24.18 Gate.setIR 0,5000,0,6 @04:44:24.27 PuckHeater.configure :default @04:44:24.35 Gate.power :heat,:ON,limit to 5000mA @04:44:24.98 Mounted lysis Cartridge @04:44:25.01 WARNING: Using default puck temperature sensor calibration @04:44:25.05 Using default cartridge heat loss curve for HeatingAccelInCarousel @04:44:27.15 SPR.command "read_sequences" @04:44:27.24 SPR.command "stats_on" @04:44:27.41 SPR.command "start 0 1" @04:44:28.12 SPR.start /var/log/methane/spr24jun1204h.spr.bz2 @04:44:30.01 Waiting for baseline SPR stabilization @04:44:30.04 Handoff.select :waste @04:44:30.21 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @04:44:36.86 Syringe tare is 52% of full scale at 22C (drifted 1%) @04:44:36.89 PinPort.in :locking @04:44:37.00 PinPort.seek :locked @04:44:37.52 Archive.in :fast @04:44:37.63 Lysis2.in :fast @04:44:37.70 Lysis1.seek 1100uL @04:44:37.77 Archive.seek :full @04:44:37.91 Lysis2.seek 1100uL @04:44:42.29 delayUntil "04:54:29.59" @04:54:29.60 Lysis2.in :DAmix @04:54:29.73 Lysis1.in :DAmix @04:54:30.32 Lysis2.seek 850uL @04:54:30.37 Lysis1.seek 850uL @04:55:05.07 delay 5 seconds @04:55:10.10 Handoff.select SPR @04:55:10.25 SPR.command "start 2 14" @04:55:11.29 SPR stabilized @04:55:11.46 delay 10 seconds @04:55:21.48 SPR.command "start 2 0" @04:55:24.66 Waiting up to 11:00 for SPR to enter state 1 @04:57:22.33 SPR.command "hold_on" @04:57:23.71 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @04:57:24.36 Lysis2.seek 550uL @04:57:24.42 Lysis1.seek 550uL @04:58:05.97 delay 15 seconds @04:58:20.99 SPR.command "hold_off" @04:58:24.37 Waiting up to 1:00:00 for SPR to enter state 7 @05:14:39.94 SPRsummary:3.2e+08RIU,56.4RIU,101RIU,44.8RIU after 1913s @05:26:06.75 delay 10 seconds @05:26:16.77 SPR.command "start 2 0" @05:26:19.44 Waiting up to 11:00 for SPR to enter state 1 @05:28:17.11 SPR.command "hold_on" @05:28:18.49 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:28:19.15 Lysis2.seek 400uL @05:28:19.25 Lysis1.seek 400uL @05:28:40.09 delay 15 seconds @05:28:55.12 SPR.command "hold_off" @05:28:58.34 Waiting up to 1:00:00 for SPR to enter state 7 @05:56:40.72 delay 10 seconds @05:56:50.75 SPR.command "start 2 0" @05:56:53.93 Waiting up to 11:00 for SPR to enter state 1 @05:58:52.02 SPR.command "hold_on" @05:58:53.41 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @05:58:54.14 Lysis2.seek 250uL @05:58:54.21 Lysis1.seek 250uL @05:59:15.08 delay 15 seconds @05:59:30.10 SPR.command "hold_off" @05:59:33.26 Waiting up to 1:00:00 for SPR to enter state 7 @06:27:15.62 delay 10 seconds @06:27:25.64 SPR.command "start 2 0" @06:27:28.33 Waiting up to 11:00 for SPR to enter state 1 @06:29:25.99 SPR.command "hold_on" @06:29:27.38 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @06:29:28.09 Lysis2.seek 100uL @06:29:28.18 Lysis1.seek 100uL @06:29:49.02 delay 15 seconds @06:30:04.05 SPR.command "hold_off" @06:30:07.22 Waiting up to 1:00:00 for SPR to enter state 7 @06:57:49.46 SPR analysis completed @06:57:57.07 Ended log spr24jun1204h.spr.bz2 @06:57:57.09 SPR.command "stats_off" @06:57:57.61 Handoff.select :close @06:57:57.79
Gate.power :heat,:OFF @06:57:57.81 Gate.power :cartridge,:OFF @06:57:57.93 PinPort.in :unlocking @06:57:58.02 PinPort.seek :clear @06:57:58.50 Carousel.in :default @06:57:58.68 Carousel.home.seek 44 @06:57:59.51 Plunge.seek :locked @06:57:59.71 Archive.in :unlocking @06:57:59.85 Archive.seek :clear @06:57:59.99 Air.in :unlocking @06:58:00.15 Air.seek :clear @06:58:00.29 Lysis1.in :unlocking @06:58:00.46 Lysis1.seek :clear @06:58:00.59 Lysis2.in :unlocking @06:58:00.73 Lysis2.seek :clear @06:58:05.17
Gate.power :analytic1,:OFF @06:58:05.19 Gate.power :motor,:OFF @06:58:05.23 Gate.power :core,:OFF @06:58:05.27 Gate.power :main,:OFF @06:58:05.31 Safely stopped and ready to power off @06:58:05.52 Gate.power :sampler,:OFF,limit to 3500mA @06:58:05.54 Gate.power :skimmer,:OFF,limit to 3500mA @06:58:09.08 Gate.power :main,:ON,limit to 5000mA @06:58:09.10 Gate.power :analytic1,:ON,limit to 4000mA @06:58:09.24 Powering core bus @06:58:11.55 SPR Module 1, FW Rev. B, FPGA Rev. C @06:58:12.02
Plunge.configure :default @06:58:12.13 SamplePump.configure :default @06:58:12.23 Toroid.configure :default @06:58:12.34 Carousel.configure :default @06:58:12.45 Abs.configure :default @06:58:12.58 PhaseSeparator.configure :default @06:58:12.68 Archive.configure :default @06:58:12.79 Air.configure :default @06:58:12.87 Lysis1.configure :default @06:58:12.94 Lysis2.configure :default @06:58:13.04 PinPort.configure :default @06:58:13.12 All elf controllers running firmware version 4.92 @06:58:13.15 Gate.power :motor,:ON,limit to 5000mA @06:58:13.43 Plunge.home.jog 0 @06:58:14.07 Air.home.jog 0 @06:58:14.19 Lysis2.home.jog 0 @06:58:14.24 Lysis1.home.jog 0 @06:58:14.29 Archive.home.jog 0 @06:58:14.32 Toroid.home.jog 0 @06:58:14.43 Carousel.home.jog 0 @06:58:14.47 PinPort.home.jog 0 @06:58:15.44 Plunge.in :homing @06:58:16.15 Plunge.jog 1596 @06:58:16.33 PinPort.in :homing @06:58:16.38 Lysis1.in :homing @06:58:16.43 Air.in :homing @06:58:16.47 Toroid.in :homing @06:58:16.51 Archive.in :homing @06:58:16.60 Lysis2.in :homing @06:58:16.90 Lysis1.jog 1397 @06:58:16.98 PinPort.jog -107 @06:58:17.03 Air.jog 1365 @06:58:17.08 Toroid.jog 613 @06:58:17.13 Lysis2.jog 1406 @06:58:17.19 Archive.jog 1368 @06:58:17.64 PinPort.setRawPosition! 0 @06:58:17.71 PinPort.seek :home @06:58:17.85 PinPort.in :default @06:58:17.96 Plunge.setRawPosition! 50 @06:58:18.03 Plunge.seek :home @06:58:18.33 Plunge.in :default @06:58:18.43 Lysis1.setRawPosition! 50 @06:58:18.57 Air.setRawPosition! 50 @06:58:18.62 Lysis1.seek :home @06:58:18.80 Air.seek :home @06:58:18.93 Lysis2.setRawPosition! 50 @06:58:18.97 Archive.setRawPosition! 50 @06:58:19.12 Lysis2.seek :home @06:58:19.15 Archive.seek :home @06:58:19.38 Air.in :default @06:58:19.41 Lysis1.in :default @06:58:19.63 Lysis2.in :default @06:58:19.66 Toroid.setRawPosition! 0 @06:58:19.71 Archive.in :default @06:58:19.88 Toroid.seek :home @06:58:20.01 Toroid.in :default @06:58:20.14 Toroid.seek :clear @06:58:21.89
Carousel.in :default @06:58:22.04 Carousel.home.seek 44 @06:58:22.34 Selecting Cartridge 43 @06:58:22.68 Carousel.seek 43 @06:58:23.23 Lysis1.in :fast @06:58:23.31 Lysis1.seek :locked @06:58:25.95 Plunge.seek :locked @06:58:26.14 Air.in :fast @06:58:26.23 Air.seek :locked @06:58:27.22
Carousel.reconfigure :locked @06:58:27.40 Powering cartridge bus @06:58:30.53 Gate.setIR 1750,5000,0,6 @06:58:30.71 Gate.setIR 0,5000,0,6 @06:58:30.79 PuckHeater.configure :default @06:58:30.89 Gate.power :heat,:ON,limit to 5000mA @06:58:31.62 Mounted lysis Cartridge @06:58:31.65 WARNING: Using default puck temperature sensor calibration @06:58:31.70 Using default cartridge heat loss curve for HeatingAccelInCarousel @06:58:33.29 SPR.command "read_sequences" @06:58:33.37 SPR.command "stats_on" @06:58:34.25 SPR.command "start 0 1" @06:58:34.96 SPR.start /var/log/methane/spr24jun1206h.spr.bz2 @06:58:37.00 Waiting for baseline SPR stabilization @06:58:37.02 Handoff.select :waste @06:58:37.19 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @06:58:43.82 Syringe tare is 52% of full scale at 22C (drifted 1%) @06:58:43.85 PinPort.in :locking @06:58:43.96 PinPort.seek :locked @06:58:44.49 Archive.in :fast @06:58:44.62 Lysis2.in :fast @06:58:44.65 Lysis1.seek 1100uL @06:58:44.89 Archive.seek :full @06:58:44.92 Lysis2.seek 1100uL @06:58:49.27 delayUntil "07:08:36.60" @07:08:36.61 Lysis2.in :DAmix @07:08:36.75 Lysis1.in :DAmix @07:08:37.36 Lysis2.seek 850uL @07:08:37.45 Lysis1.seek 850uL @07:09:12.08 delay 5 seconds @07:09:17.11 Handoff.select SPR @07:09:17.27 SPR.command "start 2 14" @07:09:18.27 SPR stabilized @07:09:18.45 delay 10 seconds @07:09:28.47 SPR.command "start 2 0" @07:09:31.64 Waiting up to 11:00 for SPR to enter state 1 @07:11:29.31 SPR.command "hold_on" @07:11:30.69 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @07:11:31.38 Lysis2.seek 550uL @07:11:31.42 Lysis1.seek 550uL @07:12:12.97 delay 15 seconds @07:12:27.99 SPR.command "hold_off" @07:12:31.35 Waiting up to 1:00:00 for SPR to enter state 7 @07:28:46.92 SPRsummary:13RIU,45.8RIU,54.7RIU,46.8RIU after 1913s @07:40:13.70 delay 10 seconds @07:40:23.72 SPR.command "start 2 0" @07:40:26.42 Waiting up to 11:00 for SPR to enter state 1 @07:42:24.09 SPR.command "hold_on" @07:42:25.47 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @07:42:26.11 Lysis2.seek 400uL @07:42:26.17 Lysis1.seek 400uL @07:42:47.04 delay 15 seconds @07:43:02.06 SPR.command "hold_off" @07:43:05.84 Waiting up to 1:00:00 for SPR to enter state 7 @08:10:48.65 delay 10 seconds @08:10:58.67 SPR.command "start 2 0" @08:11:01.34 Waiting up to 11:00 for SPR to enter state 1 @08:12:59.00 SPR.command "hold_on" @08:13:00.39 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:13:01.16 Lysis2.seek 250uL @08:13:01.23 Lysis1.seek 250uL @08:13:22.09 delay 15 seconds @08:13:37.11 SPR.command "hold_off" @08:13:40.76 Waiting up to 1:00:00 for SPR to enter state 7 @08:41:23.55 delay 10 seconds @08:41:33.57 SPR.command "start 2 0" @08:41:36.25 Waiting up to 11:00 for SPR to enter state 1 @08:43:33.93 SPR.command "hold_on" @08:43:35.31 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @08:43:35.96 Lysis2.seek 100uL @08:43:36.00 Lysis1.seek 100uL @08:43:56.88 delay 15 seconds @08:44:11.90 SPR.command "hold_off" @08:44:15.15 Waiting up to 1:00:00 for SPR to enter state 7 @09:11:57.38 SPR analysis completed @09:12:04.70 Ended log spr24jun1206h.spr.bz2 @09:12:04.72 SPR.command "stats_off" @09:12:05.55 Handoff.select :close @09:12:05.72
Gate.power :heat,:OFF @09:12:05.74 Gate.power :cartridge,:OFF @09:12:05.86 PinPort.in :unlocking @09:12:05.94 PinPort.seek :clear @09:12:06.44 Carousel.in :default @09:12:07.10 Carousel.home.seek 43 @09:12:07.47 Plunge.seek :locked @09:12:07.68 Archive.in :unlocking @09:12:07.84 Archive.seek :clear @09:12:07.97 Air.in :unlocking @09:12:08.12 Air.seek :clear @09:12:08.25 Lysis1.in :unlocking @09:12:08.39 Lysis1.seek :clear @09:12:08.52 Lysis2.in :unlocking @09:12:08.65 Lysis2.seek :clear @09:12:13.08
Gate.power :analytic1,:OFF @09:12:13.12 Gate.power :motor,:OFF @09:12:13.14 Gate.power :core,:OFF @09:12:13.18 Gate.power :main,:OFF @09:12:13.21 Safely stopped and ready to power off @09:12:13.87 Gate.power :sampler,:OFF,limit to 3500mA @09:12:13.89 Gate.power :skimmer,:OFF,limit to 3500mA @09:12:16.99 Gate.power :main,:ON,limit to 5000mA @09:12:17.01 Gate.power :analytic1,:ON,limit to 4000mA @09:12:17.15 Powering core bus @09:12:19.47 SPR Module 1, FW Rev. B, FPGA Rev. C @09:12:19.93
Plunge.configure :default @09:12:20.05 SamplePump.configure :default @09:12:20.15 Toroid.configure :default @09:12:20.25 Carousel.configure :default @09:12:20.35 Abs.configure :default @09:12:20.47 PhaseSeparator.configure :default @09:12:20.59 Archive.configure :default @09:12:20.71 Air.configure :default @09:12:20.78 Lysis1.configure :default @09:12:20.86 Lysis2.configure :default @09:12:20.97 PinPort.configure :default @09:12:21.04 All elf controllers running firmware version 4.92 @09:12:21.07 Gate.power :motor,:ON,limit to 5000mA @09:12:21.51 Plunge.home.jog 0 @09:12:22.13 Lysis2.home.jog 0 @09:12:22.17 Air.home.jog 0 @09:12:22.22 Archive.home.jog 0 @09:12:22.29 Carousel.home.jog 0 @09:12:22.33 Lysis1.home.jog 0 @09:12:22.39 Toroid.home.jog 0 @09:12:22.45 PinPort.home.jog 0 @09:12:23.27 Plunge.in :homing @09:12:24.15 Plunge.jog 1599 @09:12:24.37 Lysis2.in :homing @09:12:24.44 Lysis1.in :homing @09:12:24.48 Archive.in :homing @09:12:24.53 PinPort.in :homing @09:12:24.61 Toroid.in :homing @09:12:24.66 Air.in :homing @09:12:24.93 Lysis2.jog 1406 @09:12:24.97 Lysis1.jog 1396 @09:12:25.18 Archive.jog 1367 @09:12:25.29 PinPort.jog -106 @09:12:25.35 Toroid.jog 616 @09:12:25.39 Air.jog 1369 @09:12:25.76 Plunge.setRawPosition! 50 @09:12:25.79 PinPort.setRawPosition! 0 @09:12:25.94 PinPort.seek :home @09:12:25.97 Plunge.seek :home @09:12:26.20 PinPort.in :default @09:12:26.33 Lysis2.setRawPosition! 50 @09:12:26.45 Lysis1.setRawPosition! 50 @09:12:26.50 Plunge.in :default @09:12:26.53 Lysis2.seek :home @09:12:26.80 Lysis1.seek :home @09:12:26.95 Air.setRawPosition! 50 @09:12:26.98 Archive.setRawPosition! 50 @09:12:27.14 Air.seek :home @09:12:27.17 Archive.seek :home @09:12:27.40 Lysis1.in :default @09:12:27.43 Lysis2.in :default @09:12:27.64 Archive.in :default @09:12:27.68 Air.in :default @09:12:27.86 Toroid.setRawPosition! 0 @09:12:27.94 Toroid.seek :home @09:12:28.09 Toroid.in :default @09:12:28.21 Toroid.seek :clear @09:12:29.91
Carousel.in :default @09:12:30.05 Carousel.home.seek 43 @09:12:30.37 Selecting Cartridge 42 @09:12:30.71 Carousel.seek 42 @09:12:31.26 Lysis1.in :fast @09:12:31.35 Lysis1.seek :locked @09:12:33.99 Plunge.seek :locked @09:12:34.18 Air.in :fast @09:12:34.26 Air.seek :locked @09:12:35.26
Carousel.reconfigure :locked @09:12:35.44 Powering cartridge bus @09:12:38.22 Gate.setIR 1750,5000,0,6 @09:12:38.44 Gate.setIR 0,5000,0,6 @09:12:38.53 PuckHeater.configure :default @09:12:38.66 Gate.power :heat,:ON,limit to 5000mA @09:12:39.28 Mounted lysis Cartridge @09:12:39.32 WARNING: Using default puck temperature sensor calibration @09:12:39.37 Using default cartridge heat loss curve for HeatingAccelInCarousel @09:12:40.96 SPR.command "read_sequences" @09:12:41.11 SPR.command "stats_on" @09:12:41.30 SPR.command "start 0 1" @09:12:42.00 SPR.start /var/log/methane/spr24jun1209h.spr.bz2 @09:12:43.96 Waiting for baseline SPR stabilization @09:12:43.99 Handoff.select :waste @09:12:44.17 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @09:12:50.80 Syringe tare is 52% of full scale at 22C (drifted 1%) @09:12:50.85 PinPort.in :locking @09:12:50.94 PinPort.seek :locked @09:12:51.46 Archive.in :fast @09:12:51.53 Lysis2.in :fast @09:12:51.59 Lysis1.seek 1100uL @09:12:51.78 Archive.seek :full @09:12:51.86 Lysis2.seek 1100uL @09:12:56.23 delayUntil "09:22:43.53" @09:22:43.55 Lysis2.in :DAmix @09:22:43.71 Lysis1.in :DAmix @09:22:44.31 Lysis2.seek 850uL @09:22:44.40 Lysis1.seek 850uL @09:23:19.15 delay 5 seconds @09:23:24.17 Handoff.select SPR @09:23:24.33 SPR.command "start 2 14" @09:23:25.24 SPR stabilized @09:23:25.42 delay 10 seconds @09:23:35.45 SPR.command "start 2 0" @09:23:38.63 Waiting up to 11:00 for SPR to enter state 1 @09:25:36.28 SPR.command "hold_on" @09:25:38.19 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @09:25:38.85 Lysis2.seek 550uL @09:25:38.90 Lysis1.seek 550uL @09:26:20.44 delay 15 seconds @09:26:35.46 SPR.command "hold_off" @09:26:38.32 Waiting up to 1:00:00 for SPR to enter state 7 @09:42:52.94 SPRsummary:-1.21e+03RIU,-756RIU,41.3RIU,45.6RIU after 1912s @09:54:19.74 delay 10 seconds @09:54:29.76 SPR.command "start 2 0" @09:54:32.45 Waiting up to 11:00 for SPR to enter state 1 @09:56:30.12 SPR.command "hold_on" @09:56:31.50 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @09:56:32.11 Lysis2.seek 400uL @09:56:32.17 Lysis1.seek 400uL @09:56:53.08 delay 15 seconds @09:57:08.10 SPR.command "hold_off" @09:57:11.34 Waiting up to 1:00:00 for SPR to enter state 7 @10:24:53.69 delay 10 seconds @10:25:03.72 SPR.command "start 2 0" @10:25:06.41 Waiting up to 11:00 for SPR to enter state 1 @10:25:12.08 Session Begun @10:25:13.80 -> showlog @10:27:04.09 SPR.command "hold_on" @10:27:05.47 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:27:06.19 Lysis2.seek 250uL @10:27:06.29 Lysis1.seek 250uL @10:27:27.12 delay 15 seconds @10:27:42.14 SPR.command "hold_off" @10:27:45.31 Waiting up to 1:00:00 for SPR to enter state 7 @10:55:27.67 delay 10 seconds @10:55:37.69 SPR.command "start 2 0" @10:55:40.38 Waiting up to 11:00 for SPR to enter state 1 @10:57:38.06 SPR.command "hold_on" @10:57:39.44 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @10:57:40.20 Lysis2.seek 100uL @10:57:40.26 Lysis1.seek 100uL @10:58:01.16 delay 15 seconds @10:58:16.18 SPR.command "hold_off" @10:58:19.28 Waiting up to 1:00:00 for SPR to enter state 7 @11:26:01.51 SPR analysis completed @11:26:08.89 Ended log spr24jun1209h.spr.bz2 @11:26:08.91 SPR.command "stats_off" @11:26:09.69 Handoff.select :close @11:26:09.87
Gate.power :heat,:OFF @11:26:09.92 Gate.power :cartridge,:OFF @11:26:10.05 PinPort.in :unlocking @11:26:10.13 PinPort.seek :clear @11:26:10.64 Carousel.in :default @11:26:10.79 Carousel.home.seek 42 @11:26:11.16 Plunge.seek :locked @11:26:11.37 Archive.in :unlocking @11:26:11.54 Archive.seek :clear @11:26:11.69 Air.in :unlocking @11:26:11.83 Air.seek :clear @11:26:11.98 Lysis1.in :unlocking @11:26:12.13 Lysis1.seek :clear @11:26:12.28 Lysis2.in :unlocking @11:26:12.38 Lysis2.seek :clear @11:26:17.37
Gate.power :analytic1,:OFF @11:26:17.40 Gate.power :motor,:OFF @11:26:17.45 Gate.power :core,:OFF @11:26:17.51 Gate.power :main,:OFF @11:26:17.56 Safely stopped and ready to power off @11:26:18.29 Gate.power :sampler,:OFF,limit to 3500mA @11:26:18.32 Gate.power :skimmer,:OFF,limit to 3500mA @11:26:21.32 Gate.power :main,:ON,limit to 5000mA @11:26:21.34 Gate.power :analytic1,:ON,limit to 4000mA @11:26:21.50 Powering core bus @11:26:23.82 SPR Module 1, FW Rev. B, FPGA Rev. C @11:26:24.28
Plunge.configure :default @11:26:24.40 SamplePump.configure :default @11:26:24.52 Toroid.configure :default @11:26:24.67 Carousel.configure :default @11:26:24.78 Abs.configure :default @11:26:24.90 PhaseSeparator.configure :default @11:26:25.02 Archive.configure :default @11:26:25.14 Air.configure :default @11:26:25.24 Lysis1.configure :default @11:26:25.33 Lysis2.configure :default @11:26:25.44 PinPort.configure :default @11:26:25.54 All elf controllers running firmware version 4.92 @11:26:25.57 Gate.power :motor,:ON,limit to 5000mA @11:26:25.80 Plunge.home.jog 0 @11:26:26.67 Carousel.home.jog 0 @11:26:26.70 PinPort.home.jog 0 @11:26:26.76 Lysis1.home.jog 0 @11:26:26.80 Archive.home.jog 0 @11:26:26.87 Toroid.home.jog 0 @11:26:26.93 Lysis2.home.jog 0 @11:26:26.99 Air.home.jog 0 @11:26:27.89 Plunge.in :homing @11:26:28.20 Plunge.jog 1595 @11:26:28.44 Lysis2.in :homing @11:26:28.51 Lysis1.in :homing @11:26:28.60 Archive.in :homing @11:26:28.65 PinPort.in :homing @11:26:28.70 Toroid.in :homing @11:26:28.74 Air.in :homing @11:26:29.06 Lysis2.jog 1406 @11:26:29.15 Archive.jog 1368 @11:26:29.22 Lysis1.jog 1395 @11:26:29.27 PinPort.jog -106 @11:26:29.37 Air.jog 1367 @11:26:29.45 Toroid.jog 624 @11:26:29.87 PinPort.setRawPosition! 0 @11:26:29.89 Plunge.setRawPosition! 50 @11:26:30.05 PinPort.seek :home @11:26:30.08 Plunge.seek :home @11:26:30.90 Lysis2.setRawPosition! 50 @11:26:31.03 Lysis1.setRawPosition! 50 @11:26:31.07 Archive.setRawPosition! 50 @11:26:31.14 Plunge.in :default @11:26:31.19 PinPort.in :default @11:26:31.48 Lysis2.seek :home @11:26:31.54 Lysis1.seek :home @11:26:31.59 Archive.seek :home @11:26:31.94 Toroid.setRawPosition! 0 @11:26:31.98 Air.setRawPosition! 50 @11:26:32.14 Toroid.seek :home @11:26:32.21 Air.seek :home @11:26:32.50 Lysis2.in :default @11:26:32.52 Lysis1.in :default @11:26:32.59 Toroid.in :default @11:26:32.68 Archive.in :default @11:26:32.97 Air.in :default @11:26:33.34 Toroid.seek :clear @11:26:35.08
Carousel.in :default @11:26:35.25 Carousel.home.seek 42 @11:26:35.56 Selecting Cartridge 41 @11:26:35.89 Carousel.seek 41 @11:26:36.46 Lysis1.in :fast @11:26:36.58 Lysis1.seek :locked @11:26:39.24 Plunge.seek :locked @11:26:39.45 Air.in :fast @11:26:39.55 Air.seek :locked @11:26:40.58
Carousel.reconfigure :locked @11:26:40.78 Powering cartridge bus @11:26:43.44 Gate.setIR 1750,5000,0,6 @11:26:43.57 Gate.setIR 0,5000,0,6 @11:26:43.68 PuckHeater.configure :default @11:26:43.78 Gate.power :heat,:ON,limit to 5000mA @11:26:44.47 Mounted lysis Cartridge board#Q12558 @11:26:44.51 WARNING: Using default puck temperature sensor calibration @11:26:44.58 Using default cartridge heat loss curve for HeatingAccelInCarousel @11:26:46.18 SPR.command "read_sequences" @11:26:46.40 SPR.command "stats_on" @11:26:46.62 SPR.command "start 0 1" @11:26:47.30 SPR.start /var/log/methane/spr24jun1211h.spr.bz2 @11:26:49.25 Waiting for baseline SPR stabilization @11:26:49.27 Handoff.select :waste @11:26:50.05 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @11:26:56.70 Syringe tare is 52% of full scale at 22C (drifted 1%) @11:26:56.73 PinPort.in :locking @11:26:56.85 PinPort.seek :locked @11:26:57.38 Archive.in :fast @11:26:57.46 Lysis2.in :fast @11:26:57.56 Lysis1.seek 1100uL @11:26:57.66 Archive.seek :full @11:26:57.78 Lysis2.seek 1100uL @11:27:02.15 delayUntil "11:36:48.82" @11:36:48.84 Lysis2.in :DAmix @11:36:49.00 Lysis1.in :DAmix @11:36:49.57 Lysis2.seek 850uL @11:36:49.67 Lysis1.seek 850uL @11:37:24.30 delay 5 seconds @11:37:29.32 Handoff.select SPR @11:37:29.49 SPR.command "start 2 14" @11:37:30.53 SPR stabilized @11:37:30.77 delay 10 seconds @11:37:40.80 SPR.command "start 2 0" @11:37:43.90 Waiting up to 11:00 for SPR to enter state 1 @11:39:41.57 SPR.command "hold_on" @11:39:42.95 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @11:39:43.62 Lysis2.seek 550uL @11:39:43.68 Lysis1.seek 550uL @11:40:25.27 delay 15 seconds @11:40:40.29 SPR.command "hold_off" @11:40:44.13 Waiting up to 1:00:00 for SPR to enter state 7 @11:57:00.12 SPRsummary:-1.21e+03RIU,-780RIU,50.8RIU,54.3RIU after 1914s @12:08:26.91 delay 10 seconds @12:08:36.93 SPR.command "start 2 0" @12:08:39.62 Waiting up to 11:00 for SPR to enter state 1 @19:21:35.53PDT12-Jun-24
Environmental Sample Processor @19:21:37.44 >BREAK< ESP3 Gateway v1.43 for core bus @19:21:37.46 >BREAK< ESP3 Gateway v1.43 for cartridge bus @19:21:41.60 Configuration for honu by nadia@mbari.org 5/16/24 /home/methane/esp2/type/auv/honu/configure.rb @19:21:42.34 Sea pressure (Abs) tare is 6% of full scale @19:21:46.25 Polling Can every 2:00 @19:21:46.30 Loading /home/methane/esp2/bin/processAll mission @19:21:46.56 Sea pressure (Abs) tare is 6% of full scale @19:21:46.65 Sampling default goal volumes @19:21:46.68 Options: {extraDilutions: [1,1,1]} Cartridge group: standard @19:21:47.06 Gate.power :sampler,:OFF,limit to 3500mA @19:21:47.10 Gate.power :skimmer,:OFF,limit to 3500mA @19:21:47.22 Gate.power :analytic1,:ON,limit to 4000mA @19:21:48.56 SPR Module 1, FW Rev. B, FPGA Rev. C @19:21:49.73 Session Begun @19:21:49.85
Plunge.configure :default @19:21:49.93 Plunge.hold @19:21:50.02 Plunge.configure :default @19:21:50.18 SamplePump.configure :default @19:21:50.34 Toroid.configure :default @19:21:50.42 Toroid.hold @19:21:50.52 Toroid.configure :default @19:21:50.70 Carousel.configure :default @19:21:50.78 Carousel.hold @19:21:50.87 Carousel.configure :default @19:21:51.03 Abs.configure :default @19:21:51.19 PhaseSeparator.configure :default @19:21:51.34 Archive.configure :default @19:21:51.42 Archive.hold @19:21:51.51 Archive.configure :default @19:21:51.67 Air.configure :default @19:21:51.75 Air.hold @19:21:51.85 Air.configure :default @19:21:51.98 Lysis1.configure :default @19:21:52.06 Lysis1.hold @19:21:52.15 Lysis1.configure :default @19:21:52.28 Lysis2.configure :default @19:21:52.36 Lysis2.hold @19:21:52.45 Lysis2.configure :default @19:21:52.64 PinPort.configure :default @19:21:52.72 PinPort.hold @19:21:52.82 PinPort.configure :default @19:21:52.90 All elf controllers running firmware version 4.92 @19:21:54.06 Toroid.seek :clear @19:21:54.26
Carousel.in :default @19:21:54.39 Carousel.home.seek 41 @19:21:54.69 Selecting Cartridge 40 @19:21:55.00 Carousel.seek 40 @19:21:55.55 Lysis1.in :fast @19:21:55.63 Lysis1.seek :locked @19:21:58.27 Plunge.seek :locked @19:21:58.45 Air.in :fast @19:21:58.53 Air.seek :locked @19:21:59.56
Carousel.reconfigure :locked @19:21:59.74 Powering cartridge bus @19:22:02.42 Gate.setIR 1750,5000,0,6 @19:22:02.55 Gate.setIR 0,5000,0,6 @19:22:02.66 PuckHeater.configure :default @19:22:02.75 Gate.power :heat,:ON,limit to 5000mA @19:22:03.44 Mounted lysis Cartridge @19:22:03.48 WARNING: Using default puck temperature sensor calibration @19:22:03.51 Using default cartridge heat loss curve for HeatingAccelInCarousel @19:22:05.09 SPR.command "read_sequences" @19:22:05.49 SPR.command "stats_on" @19:22:05.68 SPR.command "start 0 1" @19:22:06.37 SPR.start /var/log/methane/spr24jun1219h.spr.bz2 @19:22:08.34 Waiting for baseline SPR stabilization @19:22:08.37 Handoff.select :waste @19:22:08.55 Waiting up to 3:00 for Syringe to stabilize +/-0.20psi @19:22:15.20 Syringe tare is 52% of full scale at 21C (drifted 1%) @19:22:15.24 PinPort.in :locking @19:22:15.33 PinPort.seek :locked @19:22:16.39 Archive.in :fast @19:22:16.48 Lysis2.in :fast @19:22:16.63 Lysis1.seek 1100uL @19:22:16.73 Archive.seek :full @19:22:16.89 Lysis2.seek 1100uL @19:22:21.18 delayUntil "19:32:07.89" @19:32:07.91 Lysis2.in :DAmix @19:32:08.41 Lysis1.in :DAmix @19:32:09.01 Lysis2.seek 850uL @19:32:09.05 Lysis1.seek 850uL @19:32:43.64 delay 5 seconds @19:32:48.66 Handoff.select SPR @19:32:48.82 SPR.command "start 2 14" @19:32:49.62 SPR stabilized @19:32:49.79 delay 10 seconds @19:32:59.81 SPR.command "start 2 0" @19:33:02.99 Waiting up to 11:00 for SPR to enter state 1 @19:35:00.65 SPR.command "hold_on" @19:35:02.05 SPR Dilution 1 of 4 -- Experiment 2 with 300.0uL L1 + 300.0uL L2 @19:35:02.71 Lysis2.seek 550uL @19:35:02.77 Lysis1.seek 550uL @19:35:44.27 delay 15 seconds @19:35:59.30 SPR.command "hold_off" @19:36:02.70 Waiting up to 1:00:00 for SPR to enter state 7 @19:52:18.26 SPRsummary:1.81e+09RIU,318RIU,262RIU,292RIU after 1913s @20:03:45.05 delay 10 seconds @20:03:55.07 SPR.command "start 2 0" @20:03:57.77 Waiting up to 11:00 for SPR to enter state 1 @20:05:55.44 SPR.command "hold_on" @20:05:56.81 SPR Dilution 2 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:05:57.44 Lysis2.seek 400uL @20:05:57.50 Lysis1.seek 400uL @20:06:18.36 delay 15 seconds @20:06:33.38 SPR.command "hold_off" @20:06:36.67 Waiting up to 1:00:00 for SPR to enter state 7 @20:09:00.65 Session Ended @20:34:19.02 delay 10 seconds @20:34:29.04 SPR.command "start 2 0" @20:34:31.74 Waiting up to 11:00 for SPR to enter state 1 @20:36:29.40 SPR.command "hold_on" @20:36:30.79 SPR Dilution 3 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @20:36:31.45 Lysis2.seek 250uL @20:36:31.50 Lysis1.seek 250uL @20:36:52.34 delay 15 seconds @20:37:07.36 SPR.command "hold_off" @20:37:11.16 Waiting up to 1:00:00 for SPR to enter state 7 @21:04:53.94 delay 10 seconds @21:05:03.96 SPR.command "start 2 0" @21:05:06.65 Waiting up to 11:00 for SPR to enter state 1 @21:07:04.31 SPR.command "hold_on" @21:07:05.71 SPR Dilution 4 of 4 -- Experiment 2 with 150.0uL L1 + 150.0uL L2 @21:07:06.39 Lysis2.seek 100uL @21:07:06.42 Lysis1.seek 100uL @21:07:27.24 delay 15 seconds @21:07:42.26 SPR.command "hold_off" @21:07:45.56 Waiting up to 1:00:00 for SPR to enter state 7 @21:35:27.78 SPR analysis completed @21:35:35.46 Ended log spr24jun1219h.spr.bz2 @21:35:35.48 SPR.command "stats_off" @21:35:35.92 Intake.close @21:35:35.98 Exhaust.close @21:35:36.04 Bypass.close @21:35:36.15 Handoff.select :close @21:35:36.41
Gate.power :heat,:OFF @21:35:36.42 Gate.power :cartridge,:OFF @21:35:36.50 PinPort.in :unlocking @21:35:36.55 PinPort.seek :clear @21:35:37.04 Carousel.in :default @21:35:37.15 Carousel.home.seek 40 @21:35:37.47 Plunge.seek :locked @21:35:37.63 Archive.in :unlocking @21:35:37.74 Archive.seek :clear @21:35:37.85 Air.in :unlocking @21:35:37.98 Air.seek :clear @21:35:38.08 Lysis1.in :unlocking @21:35:38.72 Lysis1.seek :clear @21:35:38.84 Lysis2.in :unlocking @21:35:38.90 Lysis2.seek :clear @21:35:43.32
Gate.power :analytic1,:OFF @21:35:43.34 Gate.power :motor,:OFF @21:35:43.35 Gate.power :core,:OFF @21:35:43.36 Gate.power :main,:OFF @21:35:43.38 Safely stopped and ready to power off @21:35:44.01 Cartridge::None in MAIN -- Not enough standard cartridges remain