@13:04:55.39EST09-Mar-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:04:57.90 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:05:01.40 Instrument::Error in MAIN -- FlowMeter unavailable because serial port /dev/flowMeter missing @13:05:01.44 Polling Can every 10:00 @13:05:03.08 CS.configure :default @13:05:03.12 CS.hold @13:05:03.19 CS.configure :default @13:05:03.27 PS.configure :default @13:05:03.31 PS.hold @13:05:03.37 PS.configure :default @13:05:03.45 SS.configure :default @13:05:03.49 SS.hold @13:05:03.54 SS.configure :default @13:05:03.62 CC.configure :default @13:05:03.66 CC.hold @13:05:03.72 CC.configure :default @13:05:03.81 PC.configure :default @13:05:03.84 PC.hold @13:05:03.89 PC.configure :default @13:05:03.99 Forearm.configure :loose @13:05:04.03 Forearm.hold @13:05:04.08 Forearm.configure :loose @13:05:04.16 Elbow.configure :loose @13:05:04.20 Elbow.hold @13:05:04.25 Elbow.configure :loose @13:05:04.35 SC.configure :loose @13:05:04.39 SC.hold @13:05:04.44 SC.configure :loose @13:05:04.52 SE.configure :default @13:05:04.56 SE.hold @13:05:04.61 SE.configure :default @13:05:04.65 CH.coast @13:05:04.70 CH.configure :default @13:05:04.72 PH.coast @13:05:04.77 PH.configure :default @13:05:04.80 All dwarf controllers running firmware version 3.85 @13:05:04.92 Cleanly exited at 13:04:00EST09-Mar-21 @13:05:04.95 Loading /home/esp/esp2/mission/DA_03092021.rb mission @13:05:04.96 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @13:05:04.98 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/DA_03092021.rb mission" @13:05:05.00 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @13:08:52.03EST09-Mar-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:08:54.57 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:08:58.05 Instrument::Error in MAIN -- FlowMeter unavailable because serial port /dev/flowMeter missing @13:08:58.09 Polling Can every 10:00 @13:08:59.74 CS.configure :default @13:08:59.78 CS.hold @13:08:59.85 CS.configure :default @13:08:59.93 PS.configure :default @13:08:59.97 PS.hold @13:09:00.02 PS.configure :default @13:09:00.10 SS.configure :default @13:09:00.14 SS.hold @13:09:00.18 SS.configure :default @13:09:00.26 CC.configure :default @13:09:00.30 CC.hold @13:09:00.35 CC.configure :default @13:09:00.43 PC.configure :default @13:09:00.47 PC.hold @13:09:00.52 PC.configure :default @13:09:00.61 Forearm.configure :loose @13:09:00.65 Forearm.hold @13:09:00.69 Forearm.configure :loose @13:09:00.77 Elbow.configure :loose @13:09:00.81 Elbow.hold @13:09:00.85 Elbow.configure :loose @13:09:00.95 SC.configure :loose @13:09:00.99 SC.hold @13:09:01.04 SC.configure :loose @13:09:01.12 SE.configure :default @13:09:01.16 SE.hold @13:09:01.21 SE.configure :default @13:09:01.25 CH.coast @13:09:01.29 CH.configure :default @13:09:01.32 PH.coast @13:09:01.37 PH.configure :default @13:09:01.40 All dwarf controllers running firmware version 3.85 @13:09:01.50 Cleanly exited at 13:08:37EST09-Mar-21 @13:09:01.53 Loading /home/esp/esp2/mission/DA_03092021.rb mission @13:09:01.58 GoodNews.email "Starting DA_03092021 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@13:08:58, 0% flow, 24.7C, 14% humidity, 15.0psia, 13.535V, 0.423A, 0.405A avg, 5.73W",Subject: "DA_03092021 begun" @13:09:01.61
Preparing to sleep until + 00:00:05 @13:09:01.66 CS.coast @13:09:01.68
Asleep til + 00:00:05 @13:09:01.72 Continuing at 13:09 @13:09:01.73 PS.coast @13:09:01.81 SS.coast @13:09:01.85 CC.coast @13:09:01.88 PC.coast @13:09:01.91 Forearm.coast @13:09:01.94 Elbow.coast @13:09:01.97 SC.coast @13:09:02.00 SE.coast @13:09:02.38 Gate.power :core,:OFF @13:09:02.40 Gate.power :raw,:OFF @13:09:02.41 Shutdown @13:09:05.64 GoodNews.email "Then, in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_03092021.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:09:01, 0% flow, 24.8C, 14% humidity, 15.0psia, 13.539V, 0.405A, 0.393A avg, 5.49W",Subject: "Asleep until 13:09" @13:09:06.57
Powering core bus @13:09:11.63 Gate.power :raw,:ON @13:09:12.44 CS.configure :default @13:09:12.53 PS.configure :default @13:09:12.61 SS.configure :default @13:09:12.69 CC.configure :default @13:09:12.77 PC.configure :default @13:09:12.87 Forearm.configure :loose @13:09:12.95 Elbow.configure :loose @13:09:13.05 SC.configure :loose @13:09:13.13 SE.configure :default @13:09:13.17 CH.coast @13:09:13.21 CH.configure :default @13:09:13.24 PH.coast @13:09:13.29 PH.configure :default @13:09:13.33 All dwarf controllers running firmware version 3.85 @13:09:13.43 Valve.reset @13:09:13.67 Intake.select :close @13:09:13.73 Exhaust.select :close @13:09:13.74 CTV.dial :air @13:09:13.83 PSV.dial :air @13:09:14.16 CSV.dial :waste1 @13:09:14.30
Forearm.reconfigure :loose @13:09:14.34 Forearm.home.seek :retract @13:09:14.70 SSV.dial :spit,avoiding: :exhaust @13:09:14.83 CS.reconfigure :fast @13:09:14.86 SS.reconfigure :default @13:09:14.93 CS.home.seek 0ml @13:09:14.99 SS.home.seek 0ml @13:09:15.07 PS.reconfigure :default @13:09:15.13 PS.home.seek 0ml @13:09:15.18 Hand.close @13:09:15.21
Elbow.jog 1500 @13:09:15.78 Elbow.reconfigure :loose @13:09:15.82 Elbow.home.seek :clear @13:09:19.02 SE.reconfigure :default @13:09:19.06 SE.home.seek :down @13:09:19.75 SC.reconfigure :sloppy @13:09:19.79 SC.home.seek :initial @13:09:23.41 PS.coast @13:09:45.18
Forearm.seek :retract @13:09:45.25 Elbow.seek :hammer @13:09:46.28 Forearm.reconfigure :tight @13:09:46.32 Forearm.seek retracted @13:09:46.57 PC.reconfigure :default @13:09:46.60
CC.reconfigure :default @13:09:46.67 PC.home.seek :open @13:09:46.71
CC.home.seek :open @13:09:50.03 Phase [initialPurge; da #da and wsr are run simultanelously] @13:09:50.05 Notice.email "At 13:09:49 began in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_03092021.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:09:50, 0% flow, 24.7C, 14% humidity, 15.0psia, 13.515V, 0.484A, 0.472A avg, 6.54W",Subject: "Started DA_03092021 phase initialPurge; da #da and wsr are run simultanelously" @13:09:50.08
Starting initialPurge @13:09:50.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:09:50.16 Hand.open @13:09:50.87 Elbow.reconfigure :tight @13:09:50.91 Elbow.seek FlushPuck::Garage @13:09:52.72 Elbow.reconfigure :loose @13:09:52.83 Forearm.reconfigure :loose @13:09:52.87 Forearm.seek :garage @13:09:53.83 Hand.close @13:09:54.62 Forearm.seek :retract @13:09:55.60 Elbow.reconfigure :tight @13:09:55.64 Elbow.seek CC @13:09:58.50 Elbow.reconfigure :loose @13:09:58.58 Forearm.reconfigure :tight @13:09:58.62 Forearm.seek CC @13:09:59.90 CC.seek :closed @13:10:04.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.24 @13:10:04.48 Hand.open @13:10:05.19 Forearm.reconfigure :loose @13:10:05.23 Forearm.seek :retract @13:10:06.19 Flushing the Collection Valve::Manifold @13:10:06.30 CSV.dial CSR @13:10:07.62 Pulling 4.999ml of CSR.flush @13:10:07.63 CSR.select :flush @13:10:07.74 CS.reconfigure :default @13:10:07.78 CS.seek 5ml @13:10:21.37 delay 5 seconds @13:10:26.39 CSR.select :air @13:10:26.54 CS.reconfigure :fast @13:10:26.59 CS.seek 7.5ml @13:10:33.33 CSV.dial :waste @13:10:34.62 CS.reconfigure :default @13:10:34.66 CS.seek 0ml @13:10:54.72 Purging external Intake @13:10:54.83 CSV.dial :flush @13:10:55.28 CS.reconfigure :fast @13:10:55.32 CS.seek 10ml @13:11:20.09 delay 5 seconds @13:11:25.11 CSV.dial CBV @13:11:25.15
CBV.dial CSV @13:11:25.92 CTV.dial :intake @13:11:26.72 Intake.select :open @13:11:26.83 CS.seek 0ml @13:11:50.71 Intake.select :close @13:11:50.82 CTV.dial :air @13:11:51.62 Flushing upper manifold @13:11:51.73 CSV.dial :flush @13:11:52.47 CS.seek 5ml @13:12:05.62 delay 5 seconds @13:12:10.64 CSV.dial :air @13:12:11.14 CS.seek 7ml @13:12:16.90 CSV.dial CBV @13:12:16.92
CBV.dial CSV @13:12:17.95 CTV.dial :air @13:12:17.96 CS.seek 0ml @13:12:34.72 delay 7 seconds @13:12:41.75 CS.seek 3ml @13:12:49.92 CSV.dial :waste @13:12:50.37 CS.seek 0ml @13:12:58.31 CSV.dial :flush @13:12:58.87 CS.seek 3ml @13:13:06.93 delay 5 seconds @13:13:11.95 CSV.dial :air @13:13:12.47 CS.seek 5.001ml @13:13:17.99 CSV.dial :air @13:13:18.01 CS.seek 0ml @13:13:30.31 delay 7 seconds @13:13:37.34 CS.seek 3ml @13:13:45.50 CSV.dial :waste @13:13:46.23 CS.seek 0ml @13:13:54.00 Garaging FlushPuck in Collection Clamp @13:13:54.06 Elbow.reconfigure :tight @13:13:54.10 Elbow.seek CC @13:13:54.54 Elbow.reconfigure :loose @13:13:54.62 Forearm.seek CC @13:13:55.59 Hand.close @13:13:56.27 CC.seek :open @13:14:00.88 Forearm.seek :retract @13:14:01.83 Elbow.reconfigure :tight @13:14:01.87 Elbow.seek :garage @13:14:04.77 Elbow.reconfigure :loose @13:14:04.85 Forearm.reconfigure :tight @13:14:04.89 Forearm.seek :garage @13:14:05.84 Hand.open @13:14:06.57 Forearm.reconfigure :loose @13:14:06.61 Forearm.seek :retract @13:14:07.59 da sampling at most 100ml @13:14:07.62 DAfans.email "Started da in tube 2 at 13:14:07EST09-Mar-21\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:09:50, 0% flow, 24.7C, 14% humidity, 15.0psia, 13.515V, 0.484A, 0.472A avg, 6.54W",Subject: "da sampling at most 100ml" @13:14:07.64
Loading sh1 puck from tube 2 into Collection Clamp @13:14:08.30 SC.reconfigure :tight @13:14:08.34 SC.seek 2 @13:14:15.11 SC.reconfigure :loose @13:14:15.29 Elbow.reconfigure :tight @13:14:15.33 Elbow.seek 2 @13:14:17.22 Forearm.reconfigure :tight @13:14:17.26 Forearm.seek 2 + 450 counts @13:14:18.31 SE.reconfigure :pick @13:14:18.36 SE.seek :beamBreak @13:14:21.30 SE.configure :default @13:14:21.44 Elbow.seek 2 @13:14:21.76 Forearm.seek 2 + 300 counts @13:14:22.02 Forearm.reconfigure :loose @13:14:22.06 Hand.close @13:14:22.78 SE.seek :down @13:14:22.96
Forearm.seek :retract @13:14:23.89 Elbow.seek CC @13:14:25.54 SE.coast @13:14:25.65
Elbow.reconfigure :loose @13:14:25.73 Forearm.reconfigure :tight @13:14:25.77 Forearm.seek CC @13:14:26.98 CC.seek :closed @13:14:31.54 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.27 @13:14:31.55 Hand.open @13:14:32.19 Forearm.reconfigure :loose @13:14:32.23 Forearm.seek :retract @13:14:33.25 SC.coast @13:14:33.28 collectSample 100ml w/bubble point of 24psi @13:14:33.29 Priming 7ml @13:14:33.30 Intake.select :close @13:14:33.31 Exhaust.select :close @13:14:33.32 SS.hold @13:14:33.51 Calibrating pressure and temperature sensors @13:14:33.53 CTV.dial :air @13:14:33.54 SSV.dial :spit,avoiding: :exhaust @13:14:33.56 SS.reconfigure :fast @13:14:33.61 SS.seek 5ml @13:14:36.59 SSV.dial CBV @13:14:36.62
CBV.dial SSV @13:14:37.97 delay 3.8 seconds @13:14:41.88 SSV.dial :spit @13:14:43.18 SS.seek 0ml @13:14:46.08 SSV.dial CBV @13:14:47.40 CTV.dial :intake @13:14:48.20 SSV.dial CBV @13:14:48.22
CBV.dial SSV @13:14:48.23 Allowing 40s for 7.0ml of remaining 7.0ml @13:14:48.24 Intake.select :open @13:14:50.39 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:14:50.41 SS.reconfigure :inhaling 2.20ml/s @13:14:50.45 SS.seek 7ml @13:14:56.60 Stroke completed -- waiting for pressure to equalize ... @13:14:56.61 SS.reconfigure :exhale @13:14:56.66 SS.hold @13:14:58.63 Intake.select :close @13:14:58.77 SSV.dial :exhaust @13:14:59.54 Exhaust.select :open @13:14:59.65 SS.seek 0ml @13:15:03.42 Exhaust.select :close @13:15:03.53 Sampling 100.0ml @13:15:03.54 Intake.select :close @13:15:03.55 Exhaust.select :close @13:15:03.56 SS.reconfigure :default @13:15:03.60 SS.hold @13:15:03.77 CTV.dial :intake @13:15:03.79 SSV.dial CBV @13:15:03.81
CBV.dial SSV @13:15:04.57 Allowing 130s for 25.0ml of remaining 100.0ml @13:15:04.58 Intake.select :open @13:15:06.73 Intake @15.0psia, vacuum from 9.5 to 12.5psi @13:15:06.75 SS.reconfigure :inhaling 2.20ml/s @13:15:06.79 SS.seek 25ml @13:15:22.39 Stroke completed -- waiting for pressure to equalize ... @13:15:22.40 SS.reconfigure :exhale @13:15:22.44 SS.hold @13:15:28.88 Intake.select :close @13:15:29.02 SSV.dial :exhaust @13:15:29.78 Exhaust.select :open @13:15:29.89 SS.seek 0ml @13:15:39.31 Exhaust.select :close @13:15:39.42 SSV.dial CBV @13:15:39.45
CBV.dial SSV @13:15:40.21 Allowing 130s for 25.0ml of remaining 75.0ml @13:15:40.22 Intake.select :open @13:15:42.37 Intake @15.2psia, vacuum from 9.7 to 12.7psi @13:15:42.39 SS.reconfigure :inhaling 2.20ml/s @13:15:42.43 SS.seek 25ml @13:15:57.39 Stroke completed -- waiting for pressure to equalize ... @13:15:57.40 SS.reconfigure :exhale @13:15:57.45 SS.hold @13:16:04.96 Intake.select :close @13:16:05.10 SSV.dial :exhaust @13:16:05.87 Exhaust.select :open @13:16:05.98 SS.seek 0ml @13:16:14.77 Exhaust.select :close @13:16:14.88 SSV.dial CBV @13:16:14.91
CBV.dial SSV @13:16:15.68 Allowing 130s for 25.0ml of remaining 50.0ml @13:16:15.69 Intake.select :open @13:16:17.84 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:16:17.86 SS.reconfigure :inhaling 2.20ml/s @13:16:17.90 SS.seek 25ml @13:16:32.78 Stroke completed -- waiting for pressure to equalize ... @13:16:32.79 SS.reconfigure :exhale @13:16:32.83 SS.hold @13:16:40.26 Intake.select :close @13:16:40.40 SSV.dial :exhaust @13:16:41.14 Exhaust.select :open @13:16:41.26 SS.seek 0ml @13:16:50.24 Exhaust.select :close @13:16:50.35 SSV.dial CBV @13:16:50.38
CBV.dial SSV @13:16:51.15 Allowing 130s for 25.0ml of remaining 25.0ml @13:16:51.16 Intake.select :open @13:16:53.31 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:16:53.33 SS.reconfigure :inhaling 2.20ml/s @13:16:53.37 SS.seek 25ml @13:17:08.02 Stroke completed -- waiting for pressure to equalize ... @13:17:08.03 SS.reconfigure :exhale @13:17:08.07 SS.hold @13:17:15.58 Intake.select :close @13:17:15.72 SSV.dial :exhaust @13:17:16.50 Exhaust.select :open @13:17:16.61 SS.seek 0ml @13:17:25.52 Exhaust.select :close @13:17:25.63 Sampled 100.0ml @13:17:25.65 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:17:25.71 CSV.dial :waste @13:17:25.75 CS.seek 0ml @13:17:25.84 SSV.dial CBV,avoiding: :exhaust @13:17:25.86
CBV.dial SSV @13:17:25.96 CSV.dial :air @13:17:26.70 CS.seek 10ml @13:17:27.75
CTV.dial :air @13:17:28.54 delay 15 seconds @13:17:43.56 SSV.dial :spit,avoiding: :exhaust @13:17:44.89 CTV.dial CSV,avoiding: :intake @13:17:44.92
SS.seek 0ml @13:17:45.03 SSV.dial CBV,avoiding: :exhaust @13:17:51.64 CBV.dial between SSV and waste2 @13:17:52.03 CTV.dial CSV,avoiding: :air @13:17:52.05
CSV.dial CTV @13:17:52.62 CS.reconfigure :pressurize @13:17:52.66 CS.seek 0ml @13:18:11.21 BubblePoint: Intake @39.6psia, Outlet @15.2psia @13:18:11.22 CBV.dial SSV @13:18:11.56 SS.reconfigure :default @13:18:11.60 SS.seek 10ml @13:18:17.15 delay 15 seconds @13:18:32.26 Evacuated: Intake @16.3psia, Outlet @10.2psia @13:18:32.27 SSV.dial :spit,avoiding: :exhaust @13:18:33.61 SS.reconfigure :exhale @13:18:33.65 SS.seek 0ml @13:18:38.87 SS.reconfigure :fast @13:18:38.92 SS.seek 5ml @13:18:41.84 SSV.dial CBV,avoiding: :exhaust @13:18:41.87
CBV.dial SSV @13:18:43.20 CTV.dial :air,avoiding: :intake @13:18:44.58 CSV.dial CTV @13:18:44.61
SS.seek 10ml @13:18:45.61 CSV.dial :air @13:18:46.04 CS.reconfigure :fast @13:18:46.08 CS.seek 0ml @13:18:47.07
SSV.dial :spit,avoiding: :exhaust @13:18:48.40 SS.reconfigure :exhale @13:18:48.44 SS.seek 0ml @13:18:53.80 CS.coast @13:18:53.94
SS.reconfigure :fast @13:18:53.99 SS.seek 5ml @13:18:56.86 SSV.dial CBV,avoiding: :exhaust @13:18:56.89
CBV.dial SSV @13:18:59.21 SSV.dial :spit,avoiding: :exhaust @13:19:00.53 SS.reconfigure :default @13:19:00.57 SS.seek 0ml @13:19:03.88 SS.coast @13:19:03.92 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:19:03.93 Intake.select :close @13:19:04.00 CSV.dial :waste @13:19:04.11 SSV.dial CBV,avoiding: :exhaust @13:19:04.13
CBV.dial SSV @13:19:04.75 CS.seek 0ml @13:19:04.81 CSV.dial :air @13:19:05.48
CTV.dial :air @13:19:05.49 delay 15 seconds @13:19:05.55 CS.seek 10ml @13:19:20.51
SSV.dial :spit,avoiding: :exhaust @13:19:21.84 CTV.dial CSV,avoiding: :intake @13:19:21.89
SS.reconfigure :exhale @13:19:21.95 SS.seek 0ml @13:19:22.43 SSV.dial CBV,avoiding: :exhaust @13:19:30.39 CBV.dial between SSV and waste2 @13:19:30.79 CTV.dial CSV,avoiding: :air @13:19:30.81
CSV.dial CTV @13:19:31.26 CS.reconfigure :pressurize @13:19:31.30 CS.seek 0ml @13:19:52.68 BubblePoint: Intake @38.4psia, Outlet @14.3psia @13:19:52.69 CBV.dial SSV @13:19:53.01 SS.reconfigure :default @13:19:53.05 SS.seek 10ml @13:19:58.82 delay 15 seconds @13:20:13.93 Evacuated: Intake @18.1psia, Outlet @11.9psia @13:20:13.94 SSV.dial :spit,avoiding: :exhaust @13:20:15.26 SS.reconfigure :exhale @13:20:15.30 SS.seek 0ml @13:20:20.39 SS.reconfigure :fast @13:20:20.44 SS.seek 5ml @13:20:23.34 SSV.dial CBV,avoiding: :exhaust @13:20:23.37
CBV.dial SSV @13:20:24.70 CTV.dial :air,avoiding: :intake @13:20:26.08 CSV.dial CTV @13:20:26.11
SS.seek 10ml @13:20:27.11 CSV.dial :air @13:20:27.58 CS.reconfigure :fast @13:20:27.62 CS.seek 0ml @13:20:28.57
SSV.dial :spit,avoiding: :exhaust @13:20:29.90 SS.reconfigure :exhale @13:20:29.94 SS.seek 0ml @13:20:32.77 CS.coast @13:20:35.40
SS.reconfigure :fast @13:20:35.45 SS.seek 5ml @13:20:38.29 SSV.dial CBV,avoiding: :exhaust @13:20:38.33
CBV.dial SSV @13:20:40.66 SSV.dial :spit,avoiding: :exhaust @13:20:42.00 SS.reconfigure :default @13:20:42.04 SS.seek 0ml @13:20:45.33 SS.coast @13:20:45.37 Lysing: Adding 2.0ml AqMeOH @13:20:45.38 CBV.dial between CSV and waste1,avoiding: :DBV @13:20:46.87 CTV.dial CSV @13:20:48.22 CSV.dial CSR @13:20:49.00 Pulling 1ml of CSR.AqMeOH @13:20:49.01 CSR.select :AqMeOH @13:20:49.12 CS.reconfigure :default @13:20:49.16 CS.seek 1ml @13:20:52.72 delay 5 seconds @13:20:57.74 CSR.select :air @13:20:57.90 CS.seek 6.001ml @13:21:11.72 delay 2 seconds @13:21:13.74 CSV.dial :waste2 @13:21:14.19 CS.reconfigure :fast @13:21:14.23 CS.seek 0ml @13:21:28.78 CSV.dial CSR @13:21:29.41 Pulling 2ml of CSR.AqMeOH @13:21:29.42 CSR.select :AqMeOH @13:21:29.53 CS.reconfigure :default @13:21:29.57 CS.seek 2ml @13:21:35.75 delay 5 seconds @13:21:40.77 CSR.select :air @13:21:40.93 CS.seek 5ml @13:21:49.41 delay 2 seconds @13:21:51.43 CSV.dial CTV @13:21:51.91 CS.seek 1ml @13:22:03.00 Before lysing Collection Puck Heater at 23.7C/38.1psia @13:22:03.02 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:22:03.04 CH.seek 70C,8:00 @13:23:59.25 CH.hold 10:00,70C @13:23:59.40 Begun lysing Collection Puck Heater at 68.1C/43.8psia @13:33:59.45 Ended lysing Collection Puck Heater at 70C/41.7psia @13:33:59.46 CS.seek 0ml @13:34:02.88 CTV.dial between spare and air,avoiding: :intake @13:34:03.95 CSV.dial :air @13:34:04.40 CS.seek 5ml @13:34:17.96 CSV.dial CTV @13:34:18.42 delay 2 seconds @13:34:20.44 CSV.dial :air @13:34:20.89 delay 2 seconds @13:34:22.91 CSV.dial CTV @13:34:23.37 delay 2 seconds @13:34:25.39 CSV.dial :air @13:34:25.85 delay 2 seconds @13:34:27.87 CS.seek 0ml @13:34:41.74 Puck @69.4psia after final lysis pressurization @13:34:41.75 Recovering Lysate into Collection syringe CSV.dial CBV @13:34:42.77 CBV.dial CSV @13:34:43.15 CS.seek 5ml @13:34:56.72 delay 5 seconds @13:35:01.74 CTV.dial :air @13:35:02.27 CS.seek 7ml @13:35:08.41 delay 5 seconds @13:35:13.43 CSV.dial :air2 @13:35:13.88 CS.seek 10ml @13:35:22.56 CSV.dial between air and CTV,avoiding: CBV @13:35:23.85 Lysing Complete @13:35:23.91 Storing sh1 puck in Collection Clamp to tube 1 @13:35:24.07 SC.reconfigure :tight @13:35:24.11 SC.seek 1 @13:35:27.77 SC.reconfigure :loose @13:35:27.91 SE.reconfigure :place @13:35:27.98
Elbow.reconfigure :tight @13:35:28.03 Elbow.seek CC @13:35:28.05 SE.seek :beamBreak @13:35:28.58
Elbow.reconfigure :loose @13:35:28.66 Forearm.seek CC @13:35:29.63 Hand.close @13:35:30.40 CC.seek :open @13:35:30.90 SE.configure :default @13:35:30.95 SE.seek :place @13:35:35.14
Forearm.seek :retract @13:35:36.17 Elbow.reconfigure :tight @13:35:36.21 Elbow.seek 1 @13:35:37.89 Elbow.reconfigure :limp @13:35:37.94 Forearm.seek 1 @13:35:38.98 Elbow.hold @13:35:39.04 Elbow.reconfigure :tight @13:35:39.08 Hand.open @13:35:39.75 Forearm.force -20 @13:35:39.79 Forearm.seek 1 + 318 counts @13:35:40.09 Forearm.reconfigure :tight @13:35:40.14 Forearm.seek 1 + 450 counts @13:35:40.40 SE.seek :down @13:35:41.52
Forearm.reconfigure :loose @13:35:41.56 Forearm.seek :retract @13:35:43.09 SE.coast @13:35:43.13
SC.coast @13:35:43.16 Tranferring DA @13:35:43.17 Beginning samplePassC @13:35:43.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:35:43.36 Elbow.seek FlushPuck::Garage @13:35:45.15 Elbow.reconfigure :loose @13:35:45.23 Forearm.seek :garage @13:35:46.20 Hand.close @13:35:47.00 Forearm.seek :retract @13:35:48.01 Elbow.reconfigure :tight @13:35:48.05 Elbow.seek CC @13:35:51.41 Elbow.reconfigure :loose @13:35:51.51 Forearm.reconfigure :tight @13:35:51.55 Forearm.seek CC @13:35:52.74 CC.seek :closed @13:35:57.27 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @13:35:57.33 Hand.open @13:35:57.95 Forearm.reconfigure :loose @13:35:57.99 Forearm.seek :retract @13:35:58.96 PSV.dial PSR @13:36:00.33 Pulling 1.5ml of PSR.flush @13:36:00.34 PSR.select :flush @13:36:00.46 PS.seek 1.5ml @13:36:16.64 delay 5 seconds @13:36:21.66 PSV.dial :waste1 @13:36:22.14 PS.seek 0ml @13:36:38.44 PSV.dial PSR @13:36:38.92 PSR.select :air @13:36:39.03 PS.seek 2.5ml @13:37:05.45 PRV.dial CBV,avoiding: :ab2 @13:37:05.49
CBV.dial PRV @13:37:06.87 PSV.dial :mixloop @13:37:07.89 CTV.dial :air,avoiding: :intake @13:37:07.90 PTV.dial :PRVmixing @13:37:09.22 PS.seek 0ml @13:37:35.75 PSV.dial :air @13:37:36.20 PS.seek 2.5ml @13:38:02.57 PSV.dial :mixloop @13:38:03.05 PS.seek 0ml @13:38:29.57 PSV.dial :air @13:38:30.05 PS.seek 2.5ml @13:38:56.35 PSV.dial :mixloop @13:38:56.83 PS.seek 0ml @13:39:23.23 Garaging FlushPuck in Collection Clamp @13:39:23.29 Elbow.reconfigure :tight @13:39:23.34 Elbow.seek CC @13:39:23.72 Elbow.reconfigure :loose @13:39:23.81 Forearm.seek CC @13:39:24.78 Hand.close @13:39:25.53 CC.seek :open @13:39:30.16 Forearm.seek :retract @13:39:31.20 Elbow.reconfigure :tight @13:39:31.24 Elbow.seek :garage @13:39:34.11 Elbow.reconfigure :loose @13:39:34.19 Forearm.reconfigure :tight @13:39:34.23 Forearm.seek :garage @13:39:35.26 Hand.open @13:39:35.95 Forearm.reconfigure :loose @13:39:35.99 Forearm.seek :retract @13:39:36.98 PSV.dial :mixloop @13:39:36.99 PTV.dial :PRVmixing @13:39:37.00 PRV.dial CBV @13:39:37.02
CBV.dial PRV @13:39:37.04 CTV.dial :air,avoiding: :intake @13:39:37.05 Loading lyfil puck from tube 2 into Collection Clamp @13:39:37.26 SC.reconfigure :tight @13:39:37.30 SC.seek 2 @13:39:40.85 SC.reconfigure :loose @13:39:41.03 Elbow.reconfigure :tight @13:39:41.07 Elbow.seek 2 @13:39:42.95 Forearm.reconfigure :tight @13:39:42.99 Forearm.seek 2 + 450 counts @13:39:43.97 SE.reconfigure :pick @13:39:44.02 SE.seek :beamBreak @13:39:47.09 SE.configure :default @13:39:47.23 Elbow.seek 2 @13:39:47.39 Forearm.seek 2 + 300 counts @13:39:47.67 Forearm.reconfigure :loose @13:39:47.71 Hand.close @13:39:48.41 SE.seek :down @13:39:48.59
Forearm.seek :retract @13:39:49.51 Elbow.seek CC @13:39:51.63 Elbow.reconfigure :loose @13:39:51.66 SE.coast @13:39:51.79
Forearm.reconfigure :tight @13:39:51.83 Forearm.seek CC @13:39:53.03 CC.seek :closed @13:39:57.55 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.23 @13:39:57.56 Hand.open @13:39:58.22 Forearm.reconfigure :loose @13:39:58.26 Forearm.seek :retract @13:39:59.25 SC.coast @13:39:59.28 CTV.dial CSV,avoiding: :intake @13:40:00.66 CSV.dial CTV @13:40:00.96 PS.seek 2.5ml @13:40:27.35 CS.seek 4.5ml @13:40:42.28 delay 8 seconds @13:40:50.30 PSV.dial :air @13:40:50.76 delay 2 seconds @13:40:52.78 PSV.dial :waste2 @13:40:53.51 PS.seek 0ml @13:41:19.92 CBV.dial between PRV and waste1 @13:41:20.25 CSV.dial :air @13:41:20.71 CS.seek 10ml @13:41:35.81 CSV.dial CTV @13:41:36.27 CS.seek 0ml @13:42:02.68 CBV.dial :waste1 @13:42:03.00 delay 5 seconds @13:42:08.02 CTV.dial :air,avoiding: :intake @13:42:09.44 Storing lyfil puck in Collection Clamp to tube 1 @13:42:09.60 SC.reconfigure :tight @13:42:09.64 SC.seek 1 @13:42:13.19 SC.reconfigure :loose @13:42:13.38 Elbow.reconfigure :tight @13:42:13.42 Elbow.seek 1 @13:42:15.25 Forearm.reconfigure :tight @13:42:15.29 Forearm.seek 1 + 450 counts @13:42:16.32 SE.reconfigure :pick @13:42:16.36 SE.seek :beamBreak @13:42:19.44 SE.configure :default @13:42:19.58 Elbow.reconfigure :limp @13:42:19.62 Forearm.reconfigure :loose @13:42:19.66 Forearm.seek 1 @13:42:19.87 Elbow.hold @13:42:19.93 Elbow.reconfigure :tight @13:42:20.01 SE.seek 0.15 Pucks below Top Plate @13:42:20.45 Forearm.force -20 @13:42:20.48 Forearm.seek 1 + 318 counts @13:42:20.84 Forearm.reconfigure :tight @13:42:20.88 Forearm.seek 1 + 450 counts @13:42:21.03 SE.seek 1.15 Pucks below Top Plate @13:42:21.54 Forearm.reconfigure :loose @13:42:21.58 Forearm.seek :retract @13:42:22.48 Elbow.seek CC @13:42:24.19 Elbow.reconfigure :loose @13:42:24.27 Forearm.seek CC @13:42:25.29 Hand.close @13:42:26.03 CC.seek :open @13:42:30.74 Forearm.seek :retract @13:42:31.72 Elbow.reconfigure :tight @13:42:31.76 Elbow.seek 1 @13:42:33.58 Elbow.reconfigure :limp @13:42:33.63 Forearm.seek 1 @13:42:34.64 Elbow.hold @13:42:34.70 Elbow.reconfigure :tight @13:42:34.74 Hand.open @13:42:35.50 Forearm.force -20 @13:42:35.55 Forearm.seek 1 + 316 counts @13:42:36.36 Forearm.reconfigure :tight @13:42:36.40 Forearm.seek 1 + 450 counts @13:42:36.70 SE.seek :down @13:42:37.83
Forearm.reconfigure :loose @13:42:37.87 Forearm.seek :retract @13:42:39.39 SE.coast @13:42:39.43
SC.coast @13:42:39.46 PSV.dial :air @13:42:40.22 PS.seek 2.5ml @13:43:06.64 delay 1 second @13:43:07.66 PSV.dial :mixloop @13:43:08.09 delay 1 second @13:43:09.11 PSV.dial :air @13:43:09.58 delay 1 second @13:43:10.60 PSV.dial :mixloop @13:43:11.06 delay 1 second @13:43:12.08 PSV.dial :air @13:43:12.55 delay 1 second @13:43:13.57 PS.seek 0ml @13:43:40.08 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:43:40.25 Elbow.seek FlushPuck::Garage @13:43:42.08 Elbow.reconfigure :loose @13:43:42.16 Forearm.seek :garage @13:43:43.11 Hand.close @13:43:44.01 Forearm.seek :retract @13:43:44.98 Elbow.reconfigure :tight @13:43:45.02 Elbow.seek PC @13:43:46.27 Elbow.reconfigure :loose @13:43:46.36 Forearm.reconfigure :tight @13:43:46.40 Forearm.seek PC @13:43:47.63 PC.seek :closed @13:43:52.26 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @13:43:52.32 Hand.open @13:43:52.95 Forearm.reconfigure :loose @13:43:52.99 Forearm.seek :retract @13:43:53.97 PRV.dial :air @13:43:54.44 PSV.dial :mixloop @13:43:54.97 PS.seek 0.3ml @13:43:58.85 PSV.dial PSR @13:43:59.95 Pulling 0.4ml of PSR.air @13:43:59.96 PSR.select :air @13:43:59.98 PS.seek 0.7ml @13:44:04.92 delay 3 seconds @13:44:07.94 PSR.select :air @13:44:07.96 PS.seek 2.5ml @13:44:27.17 delay 3 seconds @13:44:30.19 PSV.dial :mixloop @13:44:31.36 PTV.dial :mixing @13:44:32.11 PBV.dial :waste2 @13:44:33.25 PS.seek 0ml @13:44:59.67 Garaging FlushPuck in Processing Clamp @13:44:59.73 Elbow.reconfigure :tight @13:44:59.77 Elbow.seek PC @13:44:59.89 Elbow.reconfigure :loose @13:44:59.97 Forearm.seek PC @13:45:00.94 Hand.close @13:45:01.65 PC.seek :open @13:45:06.40 Forearm.seek :retract @13:45:07.37 Elbow.reconfigure :tight @13:45:07.41 Elbow.seek :garage @13:45:08.78 Elbow.reconfigure :loose @13:45:08.86 Forearm.reconfigure :tight @13:45:08.91 Forearm.seek :garage @13:45:09.86 Hand.open @13:45:10.58 Forearm.reconfigure :loose @13:45:10.62 Forearm.seek :retract @13:45:11.60 Loading da puck from tube 2 into Processing Clamp @13:45:11.81 SC.reconfigure :tight @13:45:11.85 SC.seek 2 @13:45:15.40 SC.reconfigure :loose @13:45:15.58 Elbow.reconfigure :tight @13:45:15.62 Elbow.seek 2 @13:45:17.46 Forearm.reconfigure :tight @13:45:17.51 Forearm.seek 2 + 450 counts @13:45:18.52 SE.reconfigure :pick @13:45:18.57 SE.seek :beamBreak @13:45:22.08 SE.configure :default @13:45:22.56 Elbow.seek 2 @13:45:22.80 Forearm.seek 2 + 300 counts @13:45:23.11 Forearm.reconfigure :loose @13:45:23.15 Hand.close @13:45:23.91 Puck::Warning in MAIN -- Loaded Last remaining Puck (from Tube 2) @13:45:23.93 SE.seek :down @13:45:24.12
Forearm.seek :retract @13:45:25.09 Elbow.seek PC @13:45:26.78 SE.coast @13:45:27.89
Elbow.reconfigure :loose @13:45:27.98 Forearm.reconfigure :tight @13:45:28.03 Forearm.seek PC @13:45:29.23 PC.seek :closed @13:45:33.84 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @13:45:33.85 Hand.open @13:45:34.45 Forearm.reconfigure :loose @13:45:34.49 Forearm.seek :retract @13:45:35.50 SC.coast @13:45:35.53 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @13:45:35.55 PH.seek 28C,5:00 @13:46:10.36 PH.hold 2:00:00,28C @13:46:10.40 delay 1:30 @13:47:40.45 PSR.select :air @13:47:40.46 PSV.dial PSR @13:47:41.55 Pulling 0.5ml of PSR.wash @13:47:41.56 PSR.select :wash @13:47:41.67 PS.seek 0.5ml @13:47:47.58 delay 5 seconds @13:47:52.60 PSR.select :air @13:47:52.72 PS.seek 2ml @13:48:08.82 PTV.dial :puck @13:48:09.56 PSV.dial PTV @13:48:10.44 PBV.dial :waste1 @13:48:11.82 PS.seek 1.5ml @13:48:17.80 delay 3 seconds @13:48:20.82 PBV.dial between PSV and waste1 @13:48:21.17 PS.seek 0ml @13:48:37.26 delay 5:00 @13:53:37.28 recoverPSRreagent08 @13:53:37.29 PTV.dial :puck @13:53:37.30 PBV.dial :waste1 @13:53:37.65 delay 30 seconds @13:54:07.67 PBV.dial between PSV and waste1 @13:54:08.03 PSR.select :air @13:54:08.04 PSV.dial PSR @13:54:08.78 PS.reconfigure :fast @13:54:08.82 PS.seek 2.5ml @13:54:32.70 PSV.dial PTV @13:54:33.45 delay 5 seconds @13:54:38.47 PSV.dial PSR @13:54:39.22 delay 5 seconds @13:54:44.24 PSV.dial PTV @13:54:44.98 delay 5 seconds @13:54:50.00 PSV.dial :air @13:54:50.89 PS.seek 0ml @13:55:14.17 PTV.dial :PRVmixing,via: PRV @13:55:15.48 PSV.dial :mixloop @13:55:16.03 PRV.dial :ab1 @13:55:16.78 PS.reconfigure :slow @13:55:16.82 PS.seek 0.15ml @13:55:20.36 delay 2 seconds @13:55:22.38 CBV.dial PRV @13:55:22.41
PRV.dial CBV @13:55:22.96 PS.seek 0.36ml @13:55:27.70 delay 2 seconds @13:55:29.72 CBV.dial between PRV and PSV @13:55:30.04 PRV.dial :air @13:55:30.48 PS.seek 0.9ml @13:55:41.97 PRV.dial :waste2 @13:55:42.72 PS.seek 0.5ml @13:55:51.45 addPMLReagent08 @13:55:51.46 PSV.dial :air @13:55:51.90 PS.reconfigure :default @13:55:51.94 PS.seek 2ml @13:56:08.13 PSV.dial :PML @13:56:09.06 PTV.dial :mixing @13:56:09.81 PBV.dial :waste1 @13:56:10.25 PS.seek 1.85ml @13:56:12.61 PBV.dial between PSV and waste1 @13:56:12.98 PS.seek 0ml @13:56:32.89 delay 2 seconds @13:56:34.91 Beginning samplePassC @13:56:34.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:56:35.08 Elbow.reconfigure :tight @13:56:35.12 Elbow.seek FlushPuck::Garage @13:56:36.44 Elbow.reconfigure :loose @13:56:36.53 Forearm.seek :garage @13:56:37.49 Hand.close @13:56:38.40 Forearm.seek :retract @13:56:39.35 Elbow.reconfigure :tight @13:56:39.39 Elbow.seek CC @13:56:42.19 Elbow.reconfigure :loose @13:56:42.28 Forearm.reconfigure :tight @13:56:42.32 Forearm.seek CC @13:56:43.51 CC.seek :closed @13:56:48.03 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @13:56:48.09 Hand.open @13:56:48.73 Forearm.reconfigure :loose @13:56:48.77 Forearm.seek :retract @13:56:49.71 PSV.dial PSR @13:56:50.94 Pulling 1.5ml of PSR.flush @13:56:50.95 PSR.select :flush @13:56:51.07 PS.seek 1.5ml @13:57:07.23 delay 5 seconds @13:57:12.25 PSV.dial :waste1 @13:57:12.73 PS.seek 0ml @13:57:28.86 PSV.dial PSR @13:57:29.33 PSR.select :air @13:57:29.44 PS.seek 2.5ml @13:57:55.86 PRV.dial CBV,avoiding: :ab2 @13:57:55.89
CBV.dial PRV @13:57:57.50 PSV.dial :mixloop @13:57:58.53 CTV.dial :air,avoiding: :intake @13:57:58.54 PTV.dial :PRVmixing @13:57:59.31 PS.seek 0ml @13:58:25.67 PSV.dial :air @13:58:26.14 PS.seek 2.5ml @13:58:52.51 PSV.dial :mixloop @13:58:53.09 PS.seek 0ml @13:59:19.41 PSV.dial :air @13:59:20.01 PS.seek 2.5ml @13:59:46.35 PSV.dial :mixloop @13:59:46.81 PS.seek 0ml @14:00:13.14 Garaging FlushPuck in Collection Clamp @14:00:13.21 Elbow.reconfigure :tight @14:00:13.25 Elbow.seek CC @14:00:13.60 Elbow.reconfigure :loose @14:00:13.68 Forearm.seek CC @14:00:14.64 Hand.close @14:00:15.41 CC.seek :open @14:00:20.01 Forearm.seek :retract @14:00:20.96 Elbow.reconfigure :tight @14:00:21.00 Elbow.seek :garage @14:00:23.79 Elbow.reconfigure :loose @14:00:23.88 Forearm.reconfigure :tight @14:00:23.92 Forearm.seek :garage @14:00:24.97 Hand.open @14:00:25.59 Forearm.reconfigure :loose @14:00:25.63 Forearm.seek :retract @14:00:26.58 delayUntil "14:04:34.89" @14:04:34.91 recoverPRVreagent08 @14:04:34.92 PTV.dial :mixing @14:04:35.67 PBV.dial :waste1 @14:04:36.05 delay 30 seconds @14:05:06.08 PBV.dial between PSV and waste1 @14:05:06.45 PSV.dial :air @14:05:06.92 PS.reconfigure :fast @14:05:06.96 PS.seek 2.5ml @14:05:30.88 PSV.dial :mixloop @14:05:31.36 delay 2 seconds @14:05:33.38 PSV.dial :air @14:05:33.84 delay 2 seconds @14:05:35.86 PSV.dial :mixloop @14:05:36.80 delay 2 seconds @14:05:38.82 PSV.dial :air @14:05:39.28 PS.seek 0ml @14:06:02.51 washCycleDA #1 of 2 @14:06:02.52 PSR.select :air @14:06:02.53 PSV.dial PSR @14:06:03.89 Pulling 0.5ml of PSR.wash @14:06:03.90 PSR.select :wash @14:06:04.01 PS.reconfigure :default @14:06:04.05 PS.seek 0.5ml @14:06:10.08 delay 5 seconds @14:06:15.10 PSR.select :air @14:06:15.22 PS.seek 2ml @14:06:31.38 PTV.dial :puck @14:06:32.14 PSV.dial PTV @14:06:33.00 PBV.dial :waste1 @14:06:33.42 PS.seek 1.5ml @14:06:39.42 delay 3 seconds @14:06:42.44 PBV.dial between PSV and waste1 @14:06:42.81 PS.seek 0ml @14:06:59.14 delay 2:00 @14:08:59.17 recoverPSRreagent08 @14:08:59.18 PTV.dial :puck @14:08:59.19 PBV.dial :waste1 @14:08:59.56 delay 30 seconds @14:09:29.59 PBV.dial between PSV and waste1 @14:09:29.97 PSR.select :air @14:09:29.98 PSV.dial PSR @14:09:30.73 PS.reconfigure :fast @14:09:30.78 PS.seek 2.5ml @14:09:54.85 PSV.dial PTV @14:09:55.62 delay 5 seconds @14:10:00.64 PSV.dial PSR @14:10:01.39 delay 5 seconds @14:10:06.41 PSV.dial PTV @14:10:07.15 delay 5 seconds @14:10:12.17 PSV.dial :air @14:10:12.92 PS.seek 0ml @14:10:36.45 washCycleDA #2 of 2 @14:10:36.46 PSR.select :air @14:10:36.47 PSV.dial PSR @14:10:37.83 Pulling 0.5ml of PSR.wash @14:10:37.84 PSR.select :wash @14:10:37.95 PS.reconfigure :default @14:10:37.99 PS.seek 0.5ml @14:10:43.98 delay 5 seconds @14:10:49.00 PSR.select :air @14:10:49.12 PS.seek 2ml @14:11:05.22 PTV.dial :puck @14:11:05.24 PSV.dial PTV @14:11:06.09 PBV.dial :waste1 @14:11:06.52 PS.seek 1.5ml @14:11:12.51 delay 3 seconds @14:11:15.53 PBV.dial between PSV and waste1 @14:11:15.89 PS.seek 0ml @14:11:32.05 delay 2:00 @14:13:32.08 recoverPSRreagent08 @14:13:32.09 PTV.dial :puck @14:13:32.10 PBV.dial :waste1 @14:13:32.45 delay 30 seconds @14:14:02.48 PBV.dial between PSV and waste1 @14:14:02.84 PSR.select :air @14:14:02.85 PSV.dial PSR @14:14:03.59 PS.reconfigure :fast @14:14:03.63 PS.seek 2.5ml @14:14:27.50 PSV.dial PTV @14:14:28.28 delay 5 seconds @14:14:33.30 PSV.dial PSR @14:14:34.17 delay 5 seconds @14:14:39.19 PSV.dial PTV @14:14:39.92 delay 5 seconds @14:14:44.94 PSV.dial :air @14:14:45.69 PS.seek 0ml @14:15:09.05 getPMLReagent08 @14:15:09.06 PRV.dial :air @14:15:09.51 delay 5 seconds @14:15:14.53 PTV.dial :PRVmixing @14:15:15.84 delay 5 seconds @14:15:20.86 PRV.dial :ab2 @14:15:21.31 PTV.dial :PRVmixing @14:15:21.32 PSV.dial :mixloop @14:15:21.78 PS.reconfigure :default @14:15:21.82 PS.seek 0.3ml @14:15:25.73 delay 2 seconds @14:15:27.75 PRV.dial :air @14:15:28.27 PS.seek 0.55ml @14:15:31.70 addPMLReagent08 @14:15:31.71 PSV.dial :air @14:15:32.17 PS.seek 2ml @14:15:47.75 PSV.dial :PML @14:15:48.22 PTV.dial :mixing @14:15:48.97 PBV.dial :waste1 @14:15:49.38 PS.seek 1.85ml @14:15:51.70 PBV.dial between PSV and waste1 @14:15:52.06 PS.seek 0ml @14:16:11.86 delay 2 seconds @14:16:13.88 delay 8:00 @14:24:13.90 recoverPRVreagent08 @14:24:13.91 PTV.dial :mixing @14:24:13.92 PBV.dial :waste1 @14:24:14.30 delay 30 seconds @14:24:44.33 PBV.dial between PSV and waste1 @14:24:44.70 PSV.dial :air @14:24:45.17 PS.reconfigure :fast @14:24:45.21 PS.seek 2.5ml @14:25:09.19 PSV.dial :mixloop @14:25:09.67 delay 2 seconds @14:25:11.69 PSV.dial :air @14:25:12.15 delay 2 seconds @14:25:14.17 PSV.dial :mixloop @14:25:14.64 delay 2 seconds @14:25:16.66 PSV.dial :air @14:25:17.12 PS.seek 0ml @14:25:40.39 washCycleDA #1 of 3 @14:25:40.40 PSR.select :air @14:25:40.41 PSV.dial PSR @14:25:41.77 Pulling 0.5ml of PSR.wash @14:25:41.78 PSR.select :wash @14:25:41.89 PS.reconfigure :default @14:25:41.93 PS.seek 0.5ml @14:25:47.89 delay 5 seconds @14:25:52.91 PSR.select :air @14:25:53.03 PS.seek 2ml @14:26:09.22 PTV.dial :puck @14:26:09.98 PSV.dial PTV @14:26:11.19 PBV.dial :waste1 @14:26:11.61 PS.seek 1.5ml @14:26:17.55 delay 3 seconds @14:26:20.57 PBV.dial between PSV and waste1 @14:26:20.95 PS.seek 0ml @14:26:37.17 delay 2:00 @14:28:37.20 recoverPSRreagent08 @14:28:37.21 PTV.dial :puck @14:28:37.22 PBV.dial :waste1 @14:28:37.59 delay 30 seconds @14:29:07.62 PBV.dial between PSV and waste1 @14:29:08.00 PSR.select :air @14:29:08.01 PSV.dial PSR @14:29:08.78 PS.reconfigure :fast @14:29:08.82 PS.seek 2.5ml @14:29:32.67 PSV.dial PTV @14:29:33.55 delay 5 seconds @14:29:38.57 PSV.dial PSR @14:29:39.30 delay 5 seconds @14:29:44.32 PSV.dial PTV @14:29:45.06 delay 5 seconds @14:29:50.08 PSV.dial :air @14:29:50.83 PS.seek 0ml @14:30:14.14 washCycleDA #2 of 3 @14:30:14.15 PSR.select :air @14:30:14.16 PSV.dial PSR @14:30:15.52 Pulling 0.5ml of PSR.wash @14:30:15.53 PSR.select :wash @14:30:15.64 PS.reconfigure :default @14:30:15.68 PS.seek 0.5ml @14:30:21.67 delay 5 seconds @14:30:26.69 PSR.select :air @14:30:26.81 PS.seek 2ml @14:30:43.16 PTV.dial :puck @14:30:43.18 PSV.dial PTV @14:30:43.90 PBV.dial :waste1 @14:30:44.31 PS.seek 1.5ml @14:30:50.26 delay 3 seconds @14:30:53.28 PBV.dial between PSV and waste1 @14:30:53.64 PS.seek 0ml @14:31:09.86 delay 2:00 @14:33:09.89 recoverPSRreagent08 @14:33:09.90 PTV.dial :puck @14:33:09.91 PBV.dial :waste1 @14:33:10.26 delay 30 seconds @14:33:40.29 PBV.dial between PSV and waste1 @14:33:40.65 PSR.select :air @14:33:40.66 PSV.dial PSR @14:33:41.42 PS.reconfigure :fast @14:33:41.46 PS.seek 2.5ml @14:34:05.17 PSV.dial PTV @14:34:05.92 delay 5 seconds @14:34:10.94 PSV.dial PSR @14:34:11.69 delay 5 seconds @14:34:16.71 PSV.dial PTV @14:34:17.47 delay 5 seconds @14:34:22.49 PSV.dial :air @14:34:23.23 PS.seek 0ml @14:34:46.51 washCycleDA #3 of 3 @14:34:46.52 PSR.select :air @14:34:46.53 PSV.dial PSR @14:34:47.89 Pulling 0.5ml of PSR.wash @14:34:47.90 PSR.select :wash @14:34:48.01 PS.reconfigure :default @14:34:48.05 PS.seek 0.5ml @14:34:54.01 delay 5 seconds @14:34:59.03 PSR.select :air @14:34:59.15 PS.seek 2ml @14:35:15.29 PTV.dial :puck @14:35:15.31 PSV.dial PTV @14:35:16.05 PBV.dial :waste1 @14:35:16.45 PS.seek 1.5ml @14:35:22.42 delay 3 seconds @14:35:25.44 PBV.dial between PSV and waste1 @14:35:25.80 PS.seek 0ml @14:35:41.98 delay 2:00 @14:37:42.01 recoverPSRreagent08 @14:37:42.02 PTV.dial :puck @14:37:42.03 PBV.dial :waste1 @14:37:42.39 delay 30 seconds @14:38:12.42 PBV.dial between PSV and waste1 @14:38:12.78 PSR.select :air @14:38:12.79 PSV.dial PSR @14:38:13.55 PS.reconfigure :fast @14:38:13.59 PS.seek 2.5ml @14:38:37.51 PSV.dial PTV @14:38:38.39 delay 5 seconds @14:38:43.41 PSV.dial PSR @14:38:44.26 delay 5 seconds @14:38:49.28 PSV.dial PTV @14:38:50.01 delay 5 seconds @14:38:55.03 PSV.dial :air @14:38:55.92 PS.seek 0ml @14:39:19.26 Gate.power :camera,:ON @14:39:19.27 Mixing DA Substrates @14:39:19.28 PSR.select :air @14:39:19.29 PSV.dial PSR @14:39:20.87 Pulling 0.25ml of PSR.sub1 @14:39:20.89 PSR.select :sub1 @14:39:21.09 PS.reconfigure :default @14:39:21.18 PS.seek 0.25ml @14:39:24.62 delay 5 seconds @14:39:29.69 Pulling 0.25ml of PSR.sub2 @14:39:29.70 PSR.select :sub2 @14:39:29.81 PS.seek 0.5ml @14:39:33.17 delay 5 seconds @14:39:38.19 PSR.select :air @14:39:38.30 PS.seek 2ml @14:39:54.47 PTV.dial :puck @14:39:54.49 PSV.dial PTV @14:39:55.28 PBV.dial :waste1 @14:39:55.72 PS.seek 1.5ml @14:40:01.67 delay 3 seconds @14:40:04.69 PBV.dial between PSV and waste1 @14:40:05.06 PS.seek 0ml @14:40:21.20 delay 10 seconds @14:40:31.23 recoverPSRreagent08 @14:40:31.24 PTV.dial :puck @14:40:31.25 PBV.dial :waste1 @14:40:31.64 delay 30 seconds @14:41:01.67 PBV.dial between PSV and waste1 @14:41:02.06 PSR.select :air @14:41:02.07 PSV.dial PSR @14:41:02.87 PS.reconfigure :fast @14:41:02.91 PS.seek 2.5ml @14:41:27.91 PSV.dial PTV @14:41:29.19 delay 5 seconds @14:41:34.21 PSV.dial PSR @14:41:35.00 delay 5 seconds @14:41:40.02 PSV.dial PTV @14:41:40.90 delay 5 seconds @14:41:45.92 PSV.dial :air @14:41:46.73 PS.seek 0ml @14:42:11.51 Additional air clear @14:42:11.52 PSR.select :air @14:42:11.53 PSV.dial PSR @14:42:12.94 Pulling 2ml of PSR.air @14:42:12.95 PSR.select :air @14:42:12.97 PS.reconfigure :default @14:42:13.01 PS.seek 2ml @14:42:34.26 delay 5 seconds @14:42:39.28 PTV.dial :puck @14:42:39.30 PSV.dial PTV @14:42:40.06 PBV.dial :waste1 @14:42:40.50 PS.seek 1.5ml @14:42:46.48 delay 3 seconds @14:42:49.50 PBV.dial between PSV and waste1 @14:42:49.89 delay 1 second @14:42:50.91 PSV.dial :waste1 @14:42:51.98 PS.seek 0ml @14:43:08.12 Processing Puck Heater at 28.1C before imaging @14:43:08.13 PH.coast @14:43:08.25 Imaging da puck in Processing Clamp while storing it to tube 1 @14:43:08.40 SC.reconfigure :tight @14:43:08.45 SC.seek 1 @14:43:12.00 SC.reconfigure :loose @14:43:12.18 Elbow.reconfigure :tight @14:43:12.23 Elbow.seek 1 @14:43:14.07 Forearm.reconfigure :tight @14:43:14.12 Forearm.seek 1 + 450 counts @14:43:15.13 SE.reconfigure :pick @14:43:15.18 SE.seek :beamBreak @14:43:18.19 SE.configure :default @14:43:18.33 Elbow.reconfigure :limp @14:43:18.38 Forearm.reconfigure :loose @14:43:18.42 Forearm.seek 1 @14:43:18.72 Elbow.hold @14:43:18.78 Elbow.reconfigure :tight @14:43:18.86 SE.seek 1.16 Pucks below Top Plate @14:43:19.34 Forearm.force -20 @14:43:19.38 Forearm.seek 1 + 309 counts @14:43:19.80 Forearm.reconfigure :tight @14:43:19.84 Forearm.seek 1 + 450 counts @14:43:20.04 SE.seek 2.16 Pucks below Top Plate @14:43:20.59 Forearm.reconfigure :loose @14:43:20.63 Forearm.seek :retract @14:43:21.54 Elbow.seek PC @14:43:24.07 Elbow.reconfigure :loose @14:43:24.15 Forearm.seek PC @14:43:25.16 Hand.close @14:43:25.93 PC.seek :open @14:43:30.71 Forearm.seek :retract @14:43:31.72 Elbow.reconfigure :tight @14:43:31.76 Elbow.seek 1 @14:43:34.47 Forearm.reconfigure :tight @14:43:34.52 Forearm.seek 1 + 450 counts @14:43:35.46 Hand.open @14:43:36.23 SE.seek 1.81 Pucks below Top Plate @14:43:36.51 SE.reconfigure :seal @14:43:36.56 SE.seek 0.84 Pucks below Top Plate @14:43:37.09 Lights, camera, ACTION! @14:43:37.12 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21mar0914h100ml.tif @14:43:56.61 Waiting >20 seconds for da to relinquish FlushPuck @14:44:01.77 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 21.228 seconds /var/log/esp/da21mar0914h100ml.tif: TIFF Upload Complete @14:44:01.79 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21mar0914h100ml15s.tif @14:44:17.36 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21mar0914h100ml15s.tif: TIFF Upload Complete @14:44:17.38 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21mar0914h100ml30s.tif @14:44:47.95 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21mar0914h100ml30s.tif: TIFF Upload Complete @14:44:47.97 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21mar0914h100ml70s.tif @14:45:59.54 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21mar0914h100ml70s.tif: TIFF Upload Complete @14:45:59.55 Gate.power :camera,:OFF @14:45:59.57 DAfans.email "21.228 sec auto-exposure",Subject: "da21mar0914h100ml images!" @14:45:59.83 Elbow.seek 1 @14:46:00.11 Forearm.seek 1 + 450 counts @14:46:00.18 SE.reconfigure :gently @14:46:00.22 SE.seek 1.56 Pucks below Top Plate @14:46:00.82 Elbow.reconfigure :limp @14:46:00.87 Forearm.reconfigure :loose @14:46:00.91 Forearm.seek 1 @14:46:01.15 Elbow.hold @14:46:01.21 Elbow.reconfigure :tight @14:46:01.26 SE.reconfigure :default @14:46:01.30 SE.seek 1.74 Pucks below Top Plate @14:46:01.69 Forearm.force -20 @14:46:01.73 Forearm.seek 1 + 308 counts @14:46:02.64 Forearm.reconfigure :tight @14:46:02.72 Forearm.seek 1 + 450 counts @14:46:03.03 SE.seek :down @14:46:04.17 Forearm.reconfigure :loose @14:46:04.22 Forearm.seek :retract @14:46:05.61 SE.coast @14:46:05.65 SC.coast @14:46:05.74 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:46:05.77
Starting finalPurge @14:46:05.96 Elbow.seek FlushPuck::Garage @14:46:07.71 Elbow.reconfigure :loose @14:46:07.80 Forearm.seek :garage @14:46:08.80 Hand.close @14:46:09.65 Forearm.seek :retract @14:46:10.62 Elbow.reconfigure :tight @14:46:10.66 Elbow.seek PC @14:46:11.92 Elbow.reconfigure :loose @14:46:12.01 Forearm.reconfigure :tight @14:46:12.05 Forearm.seek PC @14:46:13.32 PC.seek :closed @14:46:17.94 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.62 @14:46:18.00 Hand.open @14:46:19.24 Forearm.reconfigure :loose @14:46:19.28 Forearm.seek :retract @14:46:20.26 PSR.select :air @14:46:20.28 PSV.dial PSR @14:46:20.80 Pulling 0.5ml of PSR.flush @14:46:20.81 PSR.select :flush @14:46:20.93 PS.seek 0.5ml @14:46:25.62
Waiting >20 seconds for psFlush to relinquish FlushPuck @14:46:26.83 delay 5 seconds @14:46:31.85 PSR.select :air @14:46:31.96 PS.seek 2.5ml @14:46:53.14 delay 1 second @14:46:54.16 PRV.dial :waste2,avoiding: :ab2 @14:46:56.01 PTV.dial :PRVmixing @14:46:57.33 PSV.dial :mixloop @14:46:58.37 PS.seek :empty @14:47:24.69 PSR.select :air @14:47:24.71 PSV.dial PSR @14:47:25.81 Pulling 0.5ml of PSR.flush @14:47:25.82 PSR.select :flush @14:47:25.93 PS.seek 0.5ml @14:47:31.92 delay 5 seconds @14:47:36.94 PSR.select :air @14:47:37.06 PS.seek 2.5ml @14:47:58.29 delay 1 second @14:47:59.31 PBV.dial :waste1 @14:47:59.69 PTV.dial :mixing @14:48:00.44 PSV.dial :mixloop @14:48:01.48 PS.seek :empty @14:48:27.89 PSV.dial PSR @14:48:28.94 PSR.select :flush @14:48:29.06 PS.seek 1ml @14:48:40.13 PSR.select :air @14:48:40.25 PS.seek 2.5ml @14:48:56.38 PSV.dial PTV @14:48:57.15 PTV.dial :puck @14:48:57.89 PBV.dial :waste1 @14:48:57.90 PS.seek 0ml @14:49:24.25 PSV.dial PSR @14:49:25.03 PSR.select :flush @14:49:25.15 PS.seek 0.5ml @14:49:31.19 PSR.select :air @14:49:31.31 PS.seek 2.5ml @14:49:52.53 PSV.dial PTV @14:49:53.30 PBV.dial PSV @14:49:53.86 PS.seek 0ml @14:50:20.22 PSV.dial PBV @14:50:20.70 PS.seek 2.5ml @14:50:47.07 PSV.dial :waste1 @14:50:47.81 PS.seek 0ml @14:51:14.22 PSV.dial PSR @14:51:14.70 PSR.select :air @14:51:14.72 PS.seek 2.5ml @14:51:41.13 PSV.dial PTV @14:51:41.87 PBV.dial PSV @14:51:42.34 PS.seek 0ml @14:52:08.69 PSV.dial PBV @14:52:09.17 PS.seek 2.5ml @14:52:35.50 PSV.dial :waste1 @14:52:36.25 PS.seek 0ml @14:53:02.76 PSV.dial PSR @14:53:03.23 PSR.select :flush @14:53:03.35 PS.seek 1ml @14:53:14.44 PSR.select :air @14:53:14.56 PS.seek 2.5ml @14:53:30.73 PSV.dial PTV @14:53:31.47 PTV.dial :puck @14:53:31.48 PBV.dial :waste1 @14:53:32.05 PS.seek 0ml @14:53:58.41 PSV.dial PSR @14:53:59.19 PSR.select :flush @14:53:59.31 PS.seek 0.5ml @14:54:05.28 PSR.select :air @14:54:05.40 PS.seek 2.5ml @14:54:26.72 PSV.dial PTV @14:54:27.50 PBV.dial PSV @14:54:28.06 PS.seek 0ml @14:54:54.47 PSV.dial PBV @14:54:54.95 PS.seek 2.5ml @14:55:21.36 PSV.dial :waste1 @14:55:22.09 PS.seek 0ml @14:55:48.50 PSV.dial PSR @14:55:48.98 PSR.select :air @14:55:49.00 PS.seek 2.5ml @14:56:15.41 PSV.dial PTV @14:56:16.15 PBV.dial PSV @14:56:16.16 PS.seek 0ml @14:56:42.53 PSV.dial PBV @14:56:43.00 PS.seek 2.5ml @14:57:09.35 PSV.dial :waste1 @14:57:10.09 PS.seek 0ml @14:57:36.48
Loading FlushPuck into Collection Clamp from Processing Clamp @14:57:36.60 Elbow.reconfigure :tight @14:57:36.65 Elbow.seek PC @14:57:36.76 Elbow.reconfigure :loose @14:57:36.85 Forearm.seek PC @14:57:37.82 Hand.close @14:57:38.56 PC.seek :open @14:57:43.30 Forearm.seek :retract @14:57:44.28 Elbow.reconfigure :tight @14:57:44.32 Elbow.seek CC @14:57:47.94 Elbow.reconfigure :loose @14:57:48.02 Forearm.reconfigure :tight @14:57:48.06 Forearm.seek CC @14:57:49.27 CC.seek :closed @14:57:53.79 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.34 @14:57:53.85 Hand.open @14:57:54.48 Forearm.reconfigure :loose @14:57:54.52 Forearm.seek :retract @14:57:55.54 Flushing the Collection Valve::Manifold @14:57:55.65 CSV.dial CSR @14:57:56.15 Pulling 5ml of CSR.flush @14:57:56.16 CSR.select :flush @14:57:56.27 CS.seek 5ml @14:58:10.24 delay 5 seconds @14:58:15.26 CSR.select :air @14:58:15.41 CS.reconfigure :fast @14:58:15.45 CS.seek 7.499ml @14:58:22.20 CSV.dial :waste @14:58:23.50 CS.reconfigure :default @14:58:23.54 CS.seek 0ml @14:58:43.64 Flushing lower manifold's waste1 port @14:58:43.75 CSV.dial :flush @14:58:44.21 CS.reconfigure :fast @14:58:44.25 CS.seek 5ml @14:58:57.33 delay 5 seconds @14:59:02.35 CSV.dial :air @14:59:02.87 CS.seek 6.999ml @14:59:08.49 CSV.dial CTV @14:59:08.53
CTV.dial CSV @14:59:10.06 CBV.dial :waste1 @14:59:10.49 CS.seek 0ml @14:59:27.27 Flushing lower manifold's waste2 port @14:59:27.81 CSV.dial :flush @14:59:28.58 CS.seek 5ml @14:59:41.71 delay 5 seconds @14:59:46.73 CSV.dial :air @14:59:47.27 CS.seek 6.999ml @14:59:52.92 CSV.dial CTV @14:59:52.96
CTV.dial CSV @14:59:53.38 CBV.dial :waste2 @14:59:54.42 CS.seek 0ml @15:00:11.17 Disinfecting external Intake with kill @15:00:11.28 CSV.dial CSR @15:00:11.90 Pulling 8ml of CSR.kill @15:00:11.91 CSR.select :kill @15:00:12.02 CS.seek 8ml @15:00:31.96 delay 5 seconds @15:00:36.98 CSR.select :air @15:00:37.10 CS.seek 10ml @15:00:42.83 CSV.dial CBV @15:00:42.85
CBV.dial CSV @15:00:44.25 CTV.dial :intake @15:00:45.00 Intake.select :open @15:00:45.11 CS.seek 2ml @15:01:04.20 Intake.select :close @15:01:04.31 CTV.dial :air @15:01:05.10 CS.reconfigure :default @15:01:05.14 CS.seek 0ml @15:01:11.10 CSV.dial CSR @15:01:12.19 Pulling 5ml of CSR.flush @15:01:12.20 CSR.select :flush @15:01:12.31 CS.seek 5ml @15:01:26.11 delay 5 seconds @15:01:31.13 CSR.select :air @15:01:31.25 CS.seek 10ml @15:01:44.95 CSV.dial CBV @15:01:45.97 CS.seek 0ml @15:02:12.39 Garaging FlushPuck in Collection Clamp @15:02:12.45 Elbow.reconfigure :tight @15:02:12.50 Elbow.seek CC @15:02:12.78 Elbow.reconfigure :loose @15:02:12.87 Forearm.seek CC @15:02:13.84 Hand.close @15:02:14.56 CC.seek :open @15:02:19.21 Forearm.seek :retract @15:02:20.20 Elbow.reconfigure :tight @15:02:20.24 Elbow.seek :garage @15:02:23.16 Elbow.reconfigure :loose @15:02:23.25 Forearm.reconfigure :tight @15:02:23.29 Forearm.seek :garage @15:02:24.33 Hand.open @15:02:24.99 Forearm.reconfigure :loose @15:02:25.03 Forearm.seek :retract @15:02:26.01 Completed [initialPurge; da #da and wsr are run simultanelously] begun 13:09:49 @15:02:26.05 Asleep after completing DA_03092021 assays @15:02:26.07 GoodNews.email "{1 => 3,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@14:59:00, 0% flow, 25.3C, 13% humidity, 15.1psia, 13.471V, 0.441A, 0.423A avg, 5.95W\nSampled 100.0ml",Subject: "Asleep after completing DA_03092021 assays" @13:34:07.77EST10-Mar-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:34:10.27 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:34:13.73 Instrument::Error in MAIN -- FlowMeter unavailable because serial port /dev/flowMeter missing @13:34:13.78 Polling Can every 10:00 @13:34:15.42 CS.configure :default @13:34:15.46 CS.hold @13:34:15.52 CS.configure :default @13:34:15.60 PS.configure :default @13:34:15.64 PS.hold @13:34:15.68 PS.configure :default @13:34:15.76 SS.configure :default @13:34:15.84 CC.configure :default @13:34:15.88 CC.hold @13:34:15.93 CC.configure :default @13:34:16.01 PC.configure :default @13:34:16.05 PC.hold @13:34:16.09 PC.configure :default @13:34:16.19 Forearm.configure :loose @13:34:16.23 Forearm.hold @13:34:16.28 Forearm.configure :loose @13:34:16.36 Elbow.configure :loose @13:34:16.40 Elbow.hold @13:34:16.45 Elbow.configure :loose @13:34:16.55 SC.configure :loose @13:34:16.58 SC.hold @13:34:16.63 SC.configure :loose @13:34:16.71 SE.configure :default @13:34:16.75 SE.hold @13:34:16.79 SE.configure :default @13:34:16.82 CH.coast @13:34:16.87 CH.configure :default @13:34:16.90 PH.coast @13:34:16.94 PH.configure :default @13:34:16.97 All dwarf controllers running firmware version 3.85 @13:34:17.09 Cleanly exited at 13:33:51EST10-Mar-21 @13:34:17.12 Loading /home/esp/esp2/mission/DA_03092021.rb mission @13:34:17.17 Preparing to sleep until + 00:00:05 @13:34:17.20 GoodNews.email "Starting DA_03092021 w/pucks in tube 2\n{3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:34:13, 0% flow, 23.8C, 17% humidity, 15.2psia, 13.505V, 0.363A, 0.345A avg, 4.90W",Subject: "DA_03092021 begun" @13:34:17.23 CS.coast @13:34:17.27
Asleep til + 00:00:05 @13:34:17.30 Continuing at 13:34 @13:34:17.34 PS.coast @13:34:17.42 CC.coast @13:34:17.46 PC.coast @13:34:17.49 Forearm.coast @13:34:17.52 Elbow.coast @13:34:17.55 SC.coast @13:34:17.58 SE.coast @13:34:17.95 Gate.power :core,:OFF @13:34:17.97 Gate.power :raw,:OFF Shutdown @13:34:22.15
Powering core bus @13:34:22.45 GoodNews.email "Then, in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_03092021.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:34:17, 0% flow, 23.8C, 16% humidity, 15.2psia, 13.500V, 0.357A, 0.345A avg, 4.82W",Subject: "Asleep until 13:34" @13:34:26.87
Gate.power :raw,:ON @13:34:27.76 CS.configure :default @13:34:27.85 PS.configure :default @13:34:27.93 SS.configure :default @13:34:28.01 CC.configure :default @13:34:28.09 PC.configure :default @13:34:28.19 Forearm.configure :loose @13:34:28.27 Elbow.configure :loose @13:34:28.38 SC.configure :loose @13:34:28.46 SE.configure :default @13:34:28.50 CH.coast @13:34:28.55 CH.configure :default @13:34:28.58 PH.coast @13:34:28.63 PH.configure :default @13:34:28.66 All dwarf controllers running firmware version 3.85 @13:34:28.76 Valve.reset @13:34:28.97 Intake.select :close @13:34:29.04 Exhaust.select :close @13:34:29.05 CTV.dial :air @13:34:29.14 PSV.dial :air @13:34:29.18 CSV.dial :waste1 @13:34:29.57
Forearm.reconfigure :loose @13:34:29.67 Forearm.home.seek :retract @13:34:29.76 SSV.dial :spit,avoiding: :exhaust @13:34:29.90 CS.reconfigure :fast @13:34:29.93 SS.reconfigure :default @13:34:30.00 CS.home.seek 0ml @13:34:30.04 SS.home.seek 0ml @13:34:30.24 PS.reconfigure :default @13:34:30.29 PS.home.seek 0ml @13:34:30.44 Hand.close @13:34:30.48
Elbow.jog 1500 @13:34:31.02 Elbow.reconfigure :loose @13:34:31.06 Elbow.home.seek :clear @13:34:34.24 SE.reconfigure :default @13:34:34.29 SE.home.seek :down @13:34:34.92 SC.reconfigure :sloppy @13:34:34.96 SC.home.seek :initial @13:34:38.58 PS.coast @13:35:00.22
Forearm.seek :retract @13:35:00.29 Elbow.seek :hammer @13:35:01.44 Forearm.reconfigure :tight @13:35:01.48 Forearm.seek retracted @13:35:01.74 PC.reconfigure :default @13:35:01.77
CC.reconfigure :default @13:35:01.85 CC.home.seek :open @13:35:01.87 PC.home.seek :open @13:35:05.25
Phase [initialPurge; da #da and wsr are run simultanelously] @13:35:05.26 Notice.email "At 13:35:05 began in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_03092021.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:35:05, 0% flow, 23.7C, 16% humidity, 15.2psia, 13.569V, 0.315A, 0.296A avg, 4.27W",Subject: "Started DA_03092021 phase initialPurge; da #da and wsr are run simultanelously" @13:35:05.28
Starting initialPurge @13:35:05.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:35:05.37 Hand.open @13:35:06.08 Elbow.reconfigure :tight @13:35:06.12 Elbow.seek FlushPuck::Garage @13:35:08.01 Elbow.reconfigure :loose @13:35:08.10 Forearm.reconfigure :loose @13:35:08.16 Forearm.seek :garage @13:35:09.20 Hand.close @13:35:10.08 Forearm.seek :retract @13:35:11.08 Elbow.reconfigure :tight @13:35:11.12 Elbow.seek CC @13:35:14.05 Elbow.reconfigure :loose @13:35:14.13 Forearm.reconfigure :tight @13:35:14.17 Forearm.seek CC @13:35:15.40 CC.seek :closed @13:35:19.96 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.26 @13:35:20.03 Hand.open @13:35:20.65 Forearm.reconfigure :loose @13:35:20.69 Forearm.seek :retract @13:35:21.66 Flushing the Collection Valve::Manifold @13:35:21.77 CSV.dial CSR @13:35:23.24 Pulling 5ml of CSR.flush @13:35:23.25 CSR.select :flush @13:35:23.36 CS.reconfigure :default @13:35:23.40 CS.seek 5ml @13:35:37.30 delay 5 seconds @13:35:42.32 CSR.select :air @13:35:42.48 CS.reconfigure :fast @13:35:42.52 CS.seek 7.501ml @13:35:49.55 CSV.dial :waste @13:35:50.83 CS.reconfigure :default @13:35:50.87 CS.seek 0ml @13:36:10.92 Purging external Intake @13:36:11.03 CSV.dial :flush @13:36:11.48 CS.reconfigure :fast @13:36:11.52 CS.seek 10ml @13:36:36.26 delay 5 seconds @13:36:41.28 CSV.dial CBV @13:36:41.32
CBV.dial CSV @13:36:42.09 CTV.dial :intake @13:36:42.89 Intake.select :open @13:36:43.00 CS.seek 0ml @13:37:06.85 Intake.select :close @13:37:06.96 CTV.dial :air @13:37:07.75 Flushing upper manifold @13:37:07.86 CSV.dial :flush @13:37:08.72 CS.seek 5ml @13:37:21.83 delay 5 seconds @13:37:26.85 CSV.dial :air @13:37:27.38 CS.seek 7ml @13:37:33.05 CSV.dial CBV @13:37:33.07
CBV.dial CSV @13:37:34.11 CTV.dial :air @13:37:34.12 CS.seek 0ml @13:37:50.95 delay 7 seconds @13:37:57.98 CS.seek 3ml @13:38:06.16 CSV.dial :waste @13:38:06.62 CS.seek 0ml @13:38:14.43 CSV.dial :flush @13:38:14.98 CS.seek 3ml @13:38:23.20 delay 5 seconds @13:38:28.22 CSV.dial :air @13:38:28.75 CS.seek 5.001ml @13:38:34.61 CSV.dial :air @13:38:34.62 CS.seek 0ml @13:38:46.86 delay 7 seconds @13:38:53.89 CS.seek 3ml @13:39:01.94 CSV.dial :waste @13:39:02.69 CS.seek 0ml @13:39:10.45 Garaging FlushPuck in Collection Clamp @13:39:10.51 Elbow.reconfigure :tight @13:39:10.55 Elbow.seek CC @13:39:10.87 Elbow.reconfigure :loose @13:39:10.95 Forearm.seek CC @13:39:11.97 Hand.close @13:39:12.72 CC.seek :open @13:39:17.37 Forearm.seek :retract @13:39:18.39 Elbow.reconfigure :tight @13:39:18.43 Elbow.seek :garage @13:39:21.29 Elbow.reconfigure :loose @13:39:21.38 Forearm.reconfigure :tight @13:39:21.42 Forearm.seek :garage @13:39:22.40 Hand.open @13:39:23.02 Forearm.reconfigure :loose @13:39:23.06 Forearm.seek :retract @13:39:24.05 da sampling at most 100ml @13:39:24.08 DAfans.email "Started da in tube 2 at 13:39:24EST10-Mar-21\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@13:35:05, 0% flow, 23.7C, 16% humidity, 15.2psia, 13.569V, 0.315A, 0.296A avg, 4.27W",Subject: "da sampling at most 100ml" @13:39:24.10
Loading sh1 puck from tube 2 into Collection Clamp @13:39:24.77 SC.reconfigure :tight @13:39:24.81 SC.seek 2 @13:39:31.56 SC.reconfigure :loose @13:39:31.74 Elbow.reconfigure :tight @13:39:31.78 Elbow.seek 2 @13:39:33.69 Forearm.reconfigure :tight @13:39:33.73 Forearm.seek 2 + 450 counts @13:39:34.84 SE.reconfigure :pick @13:39:34.89 SE.seek :beamBreak @13:39:37.87 SE.configure :default @13:39:38.02 Elbow.seek 2 @13:39:38.18 Forearm.seek 2 + 300 counts @13:39:38.33 Forearm.reconfigure :loose @13:39:38.37 Hand.close @13:39:39.11 SE.seek :down @13:39:39.29
Forearm.seek :retract @13:39:40.24 Elbow.seek CC @13:39:41.81 SE.coast @13:39:41.91
Elbow.reconfigure :loose @13:39:42.00 Forearm.reconfigure :tight @13:39:42.04 Forearm.seek CC @13:39:43.27 CC.seek :closed @13:39:47.80 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.22 @13:39:47.81 Hand.open @13:39:48.53 Forearm.reconfigure :loose @13:39:48.57 Forearm.seek :retract @13:39:49.53 SC.coast @13:39:49.56 collectSample 100ml w/bubble point of 24psi @13:39:49.57 Priming 7ml @13:39:49.58 Intake.select :close @13:39:49.59 Exhaust.select :close @13:39:49.60 SS.hold @13:39:49.79 Calibrating pressure and temperature sensors @13:39:49.81 CTV.dial :air @13:39:49.83 SSV.dial :spit,avoiding: :exhaust @13:39:49.84 SS.reconfigure :fast @13:39:49.89 SS.seek 5ml @13:39:52.73 SSV.dial CBV @13:39:52.76
CBV.dial SSV @13:39:54.11 delay 3.8 seconds @13:39:58.02 SSV.dial :spit @13:39:59.35 SS.seek 0ml @13:40:02.14 SSV.dial CBV @13:40:03.47 CTV.dial :intake @13:40:04.27 SSV.dial CBV @13:40:04.29
CBV.dial SSV @13:40:04.30 Allowing 40s for 7.0ml of remaining 7.0ml @13:40:04.31 Intake.select :open @13:40:06.47 Intake @15.1psia, vacuum from 9.6 to 12.6psi @13:40:06.49 SS.reconfigure :inhaling 2.20ml/s @13:40:06.53 SS.seek 7ml @13:40:12.92 Stroke completed -- waiting for pressure to equalize ... @13:40:12.93 SS.reconfigure :exhale @13:40:12.97 SS.hold @13:40:14.54 Intake.select :close @13:40:14.69 SSV.dial :exhaust @13:40:15.45 Exhaust.select :open @13:40:15.56 SS.seek 0ml @13:40:19.58 Exhaust.select :close @13:40:19.69 Sampling 100.0ml @13:40:19.70 Intake.select :close @13:40:19.71 Exhaust.select :close @13:40:19.72 SS.reconfigure :default @13:40:19.76 SS.hold @13:40:19.94 CTV.dial :intake @13:40:19.96 SSV.dial CBV @13:40:20.01
CBV.dial SSV @13:40:20.75 Allowing 130s for 25.0ml of remaining 100.0ml @13:40:20.76 Intake.select :open @13:40:22.91 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:40:22.93 SS.reconfigure :inhaling 2.20ml/s @13:40:22.97 SS.seek 25ml @13:40:38.60 Stroke completed -- waiting for pressure to equalize ... @13:40:38.61 SS.reconfigure :exhale @13:40:38.65 SS.hold @13:40:44.32 Intake.select :close @13:40:44.46 SSV.dial :exhaust @13:40:45.23 Exhaust.select :open @13:40:45.34 SS.seek 0ml @13:40:54.06 Exhaust.select :close @13:40:54.17 SSV.dial CBV @13:40:54.20
CBV.dial SSV @13:40:55.42 Allowing 130s for 25.0ml of remaining 75.0ml @13:40:55.43 Intake.select :open @13:40:57.58 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:40:57.60 SS.reconfigure :inhaling 2.20ml/s @13:40:57.64 SS.seek 25ml @13:41:13.03 Stroke completed -- waiting for pressure to equalize ... @13:41:13.04 SS.reconfigure :exhale @13:41:13.08 SS.hold @13:41:19.54 Intake.select :close @13:41:19.68 SSV.dial :exhaust @13:41:20.47 Exhaust.select :open @13:41:20.58 SS.seek 0ml @13:41:29.14 Exhaust.select :close @13:41:29.25 SSV.dial CBV @13:41:29.28
CBV.dial SSV @13:41:30.06 Allowing 130s for 25.0ml of remaining 50.0ml @13:41:30.07 Intake.select :open @13:41:32.22 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:41:32.24 SS.reconfigure :inhaling 2.20ml/s @13:41:32.28 SS.seek 25ml @13:41:47.80 Stroke completed -- waiting for pressure to equalize ... @13:41:47.81 SS.reconfigure :exhale @13:41:47.85 SS.hold @13:41:55.37 Intake.select :close @13:41:55.51 SSV.dial :exhaust @13:41:56.28 Exhaust.select :open @13:41:56.39 SS.seek 0ml @13:42:05.21 Exhaust.select :close @13:42:05.32 SSV.dial CBV @13:42:05.35
CBV.dial SSV @13:42:06.12 Allowing 130s for 25.0ml of remaining 25.0ml @13:42:06.13 Intake.select :open @13:42:08.28 Intake @15.5psia, vacuum from 10.0 to 13.0psi @13:42:08.30 SS.reconfigure :inhaling 2.20ml/s @13:42:08.34 SS.seek 24.99ml @13:42:23.56 Stroke completed -- waiting for pressure to equalize ... @13:42:23.57 SS.reconfigure :exhale @13:42:23.61 SS.hold @13:42:30.05 Intake.select :close @13:42:30.19 SSV.dial :exhaust @13:42:30.95 Exhaust.select :open @13:42:31.06 SS.seek 0ml @13:42:40.06 Exhaust.select :close @13:42:40.17 Sampled 100.0ml @13:42:40.19 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:42:40.25 CSV.dial :waste @13:42:40.28 CS.seek 0ml @13:42:40.40 SSV.dial CBV,avoiding: :exhaust @13:42:40.43 CSV.dial :air @13:42:40.45
CBV.dial SSV @13:42:41.22 CS.seek 10ml @13:42:42.34
CTV.dial :air @13:42:43.14 delay 15 seconds @13:42:58.16 SSV.dial :spit,avoiding: :exhaust @13:42:59.51 CTV.dial CSV,avoiding: :intake @13:42:59.55
SS.seek 0ml @13:42:59.65 SSV.dial CBV,avoiding: :exhaust @13:43:06.10 CBV.dial between SSV and waste2 @13:43:06.50 CTV.dial CSV,avoiding: :air @13:43:06.52
CSV.dial CTV @13:43:06.97 CS.reconfigure :pressurize @13:43:07.01 CS.seek 0ml @13:43:25.20 BubblePoint: Intake @39.8psia, Outlet @15.6psia CBV.dial SSV @13:43:25.53 SS.reconfigure :default @13:43:25.57 SS.seek 10ml @13:43:31.09 delay 15 seconds @13:43:46.20 Evacuated: Intake @16.3psia, Outlet @10.1psia @13:43:46.21 SSV.dial :spit,avoiding: :exhaust @13:43:47.54 SS.reconfigure :exhale @13:43:47.58 SS.seek 0ml @13:43:52.93 SS.reconfigure :fast @13:43:52.98 SS.seek 5ml @13:43:55.78 SSV.dial CBV,avoiding: :exhaust @13:43:55.81
CBV.dial SSV @13:43:57.15 CTV.dial :air,avoiding: :intake @13:43:58.52 CSV.dial CTV @13:43:58.55
SS.seek 10ml @13:43:59.55 CSV.dial :air @13:44:00.02 CS.reconfigure :fast @13:44:00.06 CS.seek 0ml @13:44:01.06
SSV.dial :spit,avoiding: :exhaust @13:44:02.39 SS.reconfigure :exhale @13:44:02.43 SS.seek 0ml @13:44:07.85 SS.reconfigure :fast @13:44:07.90 SS.seek 5ml @13:44:08.23 CS.coast @13:44:10.54 SSV.dial CBV,avoiding: :exhaust @13:44:10.58
CBV.dial SSV @13:44:13.00 SSV.dial :spit,avoiding: :exhaust @13:44:14.33 SS.reconfigure :default @13:44:14.37 SS.seek 0ml @13:44:17.66 SS.coast @13:44:17.70 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:44:17.71 Intake.select :close @13:44:17.78 CSV.dial :waste @13:44:17.89 SSV.dial CBV,avoiding: :exhaust @13:44:17.91
CBV.dial SSV @13:44:18.53 CS.seek 0ml @13:44:18.61 CSV.dial :air @13:44:19.26
CTV.dial :air @13:44:19.27 delay 15 seconds @13:44:19.36 CS.seek 10ml @13:44:34.29
SSV.dial :spit,avoiding: :exhaust @13:44:35.62 CTV.dial CSV,avoiding: :intake @13:44:35.66
SS.reconfigure :exhale @13:44:35.73 SS.seek 0ml @13:44:36.17 SSV.dial CBV,avoiding: :exhaust @13:44:44.35 CBV.dial between SSV and waste2 @13:44:44.75 CTV.dial CSV,avoiding: :air @13:44:44.77
CSV.dial CTV @13:44:45.24 CS.reconfigure :pressurize @13:44:45.28 CS.seek 0ml @13:45:06.58 BubblePoint: Intake @38.7psia, Outlet @14.4psia @13:45:06.59 CBV.dial SSV @13:45:06.91 SS.reconfigure :default @13:45:06.95 SS.seek 10ml @13:45:12.64 delay 15 seconds @13:45:27.75 Evacuated: Intake @18.1psia, Outlet @12.1psia @13:45:27.76 SSV.dial :spit,avoiding: :exhaust @13:45:29.09 SS.reconfigure :exhale @13:45:29.13 SS.seek 0ml @13:45:34.72 SS.reconfigure :fast @13:45:34.77 SS.seek 5ml @13:45:37.47 SSV.dial CBV,avoiding: :exhaust @13:45:37.49
CBV.dial SSV @13:45:38.84 CTV.dial :air,avoiding: :intake @13:45:40.22 CSV.dial CTV @13:45:40.25
SS.seek 10ml @13:45:41.25 CSV.dial :air @13:45:41.72 CS.reconfigure :fast @13:45:41.76 CS.seek 0ml @13:45:42.70
SSV.dial :spit,avoiding: :exhaust @13:45:44.04 SS.reconfigure :exhale @13:45:44.08 SS.seek 0ml @13:45:46.98 CS.coast @13:45:49.30
SS.reconfigure :fast @13:45:49.35 SS.seek 5ml @13:45:52.26 SSV.dial CBV,avoiding: :exhaust @13:45:52.29
CBV.dial SSV @13:45:54.65 SSV.dial :spit,avoiding: :exhaust @13:45:55.98 SS.reconfigure :default @13:45:56.02 SS.seek 0ml @13:45:59.33 SS.coast @13:45:59.37 Lysing: Adding 2.0ml AqMeOH @13:45:59.38 CBV.dial between CSV and waste1,avoiding: :DBV @13:46:00.86 CTV.dial CSV @13:46:02.20 CSV.dial CSR @13:46:03.00 Pulling 0.999ml of CSR.AqMeOH @13:46:03.01 CSR.select :AqMeOH @13:46:03.12 CS.reconfigure :default @13:46:03.16 CS.seek 1ml @13:46:06.55 delay 5 seconds @13:46:11.57 CSR.select :air @13:46:11.81 CS.seek 6ml @13:46:25.61 delay 2 seconds @13:46:27.63 CSV.dial :waste2 @13:46:28.06 CS.reconfigure :fast @13:46:28.10 CS.seek 0ml @13:46:42.66 CSV.dial CSR @13:46:43.18 Pulling 2ml of CSR.AqMeOH @13:46:43.19 CSR.select :AqMeOH @13:46:43.30 CS.reconfigure :default @13:46:43.34 CS.seek 2ml @13:46:49.24 delay 5 seconds @13:46:54.26 CSR.select :air @13:46:54.42 CS.seek 5ml @13:47:02.86 delay 2 seconds @13:47:04.88 CSV.dial CTV @13:47:05.33 CS.seek 1ml @13:47:16.58 Before lysing Collection Puck Heater at 23.6C/38.8psia @13:47:16.60 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:47:16.62 CH.seek 70C,8:00 @13:49:14.51 CH.hold 10:00,70C @13:49:14.68 Begun lysing Collection Puck Heater at 68C/43.4psia @13:59:14.71 Ended lysing Collection Puck Heater at 70C/42.2psia @13:59:14.73 CS.seek 0ml @13:59:18.18 CTV.dial between spare and air,avoiding: :intake @13:59:19.25 CSV.dial :air @13:59:19.70 CS.seek 5ml @13:59:33.45 CSV.dial CTV @13:59:33.91 delay 2 seconds @13:59:35.93 CSV.dial :air @13:59:36.38 delay 2 seconds @13:59:38.40 CSV.dial CTV @13:59:38.85 delay 2 seconds @13:59:40.87 CSV.dial :air @13:59:41.32 delay 2 seconds @13:59:43.34 CS.seek 0ml @13:59:57.04 Puck @69.6psia after final lysis pressurization @13:59:57.05 Recovering Lysate into Collection syringe @13:59:57.06 CSV.dial CBV @13:59:58.08 CBV.dial CSV @13:59:58.44 CS.seek 5ml @14:00:12.24 delay 5 seconds @14:00:17.26 CTV.dial :air @14:00:17.78 CS.seek 7.001ml @14:00:23.68 delay 5 seconds @14:00:28.70 CSV.dial :air2 @14:00:29.15 CS.seek 10ml @14:00:37.64 CSV.dial between air and CTV,avoiding: CBV @14:00:38.82 Lysing Complete @14:00:38.89 Storing sh1 puck in Collection Clamp to tube 1 @14:00:39.04 SC.reconfigure :tight @14:00:39.08 SC.seek 1 @14:00:42.70 SC.reconfigure :loose @14:00:42.85 SE.reconfigure :place @14:00:42.92
Elbow.reconfigure :tight @14:00:42.96 Elbow.seek CC @14:00:42.97 SE.seek :beamBreak @14:00:43.44
Elbow.reconfigure :loose @14:00:43.53 Forearm.seek CC @14:00:44.51 Hand.close @14:00:45.23 CC.seek :open @14:00:45.83 SE.configure :default @14:00:45.88 SE.seek :place @14:00:49.97
Forearm.seek :retract @14:00:50.95 Elbow.reconfigure :tight @14:00:50.99 Elbow.seek 1 @14:00:52.69 Elbow.reconfigure :limp @14:00:52.74 Forearm.seek 1 @14:00:53.72 Elbow.hold @14:00:53.78 Elbow.reconfigure :tight @14:00:53.82 Hand.open @14:00:54.59 Forearm.force -20 @14:00:54.62 Forearm.seek 1 + 304 counts @14:00:54.92 Forearm.reconfigure :tight @14:00:54.96 Forearm.seek 1 + 450 counts @14:00:55.20 SE.seek :down @14:00:56.33
Forearm.reconfigure :loose @14:00:56.37 Forearm.seek :retract @14:00:57.92 SE.coast @14:00:57.96
SC.coast @14:00:57.99 Tranferring DA @14:00:58.00 Beginning samplePassC @14:00:58.01 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:58.19 Elbow.seek FlushPuck::Garage @14:01:00.03 Elbow.reconfigure :loose @14:01:00.11 Forearm.seek :garage @14:01:01.07 Hand.close @14:01:01.83 Forearm.seek :retract @14:01:02.81 Elbow.reconfigure :tight @14:01:02.85 Elbow.seek CC @14:01:05.75 Elbow.reconfigure :loose @14:01:05.84 Forearm.reconfigure :tight @14:01:05.88 Forearm.seek CC @14:01:07.55 CC.seek :closed @14:01:12.07 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.39 @14:01:12.13 Hand.open @14:01:12.78 Forearm.reconfigure :loose @14:01:12.82 Forearm.seek :retract @14:01:13.78 PSV.dial PSR @14:01:15.18 Pulling 1.5ml of PSR.flush @14:01:15.19 PSR.select :flush @14:01:15.31 PS.seek 1.5ml @14:01:31.41 delay 5 seconds @14:01:36.43 PSV.dial :waste1 @14:01:36.89 PS.seek 0ml @14:01:52.99 PSV.dial PSR @14:01:53.47 PSR.select :air @14:01:53.58 PS.seek 2.5ml @14:02:20.00 PRV.dial CBV,avoiding: :ab2 @14:02:20.02
CBV.dial PRV @14:02:21.72 PSV.dial :mixloop @14:02:22.88 CTV.dial :air,avoiding: :intake @14:02:22.89 PTV.dial :PRVmixing @14:02:24.22 PS.seek 0ml @14:02:50.62 PSV.dial :air @14:02:51.10 PS.seek 2.5ml @14:03:17.46 PSV.dial :mixloop @14:03:17.94 PS.seek 0ml @14:03:44.46 PSV.dial :air @14:03:45.08 PS.seek 2.5ml @14:04:11.52 PSV.dial :mixloop @14:04:11.97 PS.seek 0ml @14:04:38.38 Garaging FlushPuck in Collection Clamp @14:04:38.44 Elbow.reconfigure :tight @14:04:38.48 Elbow.seek CC @14:04:38.83 Elbow.reconfigure :loose @14:04:38.91 Forearm.seek CC @14:04:39.87 Hand.close @14:04:40.61 CC.seek :open @14:04:45.25 Forearm.seek :retract @14:04:46.20 Elbow.reconfigure :tight @14:04:46.24 Elbow.seek :garage @14:04:49.11 Elbow.reconfigure :loose @14:04:49.20 Forearm.reconfigure :tight @14:04:49.24 Forearm.seek :garage @14:04:50.33 Hand.open @14:04:51.03 Forearm.reconfigure :loose @14:04:51.07 Forearm.seek :retract @14:04:52.05 PSV.dial :mixloop @14:04:52.06 PTV.dial :PRVmixing @14:04:52.07 PRV.dial CBV @14:04:52.09
CBV.dial PRV @14:04:52.11 CTV.dial :air,avoiding: :intake @14:04:52.12 Loading lyfil puck from tube 2 into Collection Clamp @14:04:52.34 SC.reconfigure :tight @14:04:52.38 SC.seek 2 @14:04:55.98 SC.reconfigure :loose @14:04:56.16 Elbow.reconfigure :tight @14:04:56.20 Elbow.seek 2 @14:04:58.18 Forearm.reconfigure :tight @14:04:58.22 Forearm.seek 2 + 450 counts @14:04:59.27 SE.reconfigure :pick @14:04:59.32 SE.seek :beamBreak @14:05:02.49 SE.configure :default @14:05:02.63 Elbow.seek 2 @14:05:03.08 Forearm.seek 2 + 300 counts @14:05:03.25 Forearm.reconfigure :loose @14:05:03.29 Hand.close @14:05:03.87 SE.seek :down @14:05:04.05
Forearm.seek :retract @14:05:04.98 Elbow.seek CC @14:05:06.58 SE.coast @14:05:06.69
Elbow.reconfigure :loose @14:05:06.78 Forearm.reconfigure :tight @14:05:07.27 Forearm.seek CC @14:05:08.49 CC.seek :closed @14:05:13.01 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.19 @14:05:13.02 Hand.open @14:05:13.68 Forearm.reconfigure :loose @14:05:13.72 Forearm.seek :retract @14:05:14.67 SC.coast @14:05:14.70 CTV.dial CSV,avoiding: :intake @14:05:16.08 CSV.dial CTV @14:05:16.41 PS.seek 2.5ml @14:05:42.80 CS.seek 4.5ml @14:05:57.68 delay 8 seconds @14:06:05.70 PSV.dial :air @14:06:06.31 delay 2 seconds @14:06:08.33 PSV.dial :waste2 @14:06:09.10 PS.seek 0ml @14:06:35.47 CBV.dial between PRV and waste1 @14:06:35.82 CSV.dial :air @14:06:36.29 CS.seek 10ml @14:06:51.29 CSV.dial CTV @14:06:51.88 CS.seek 0ml @14:07:18.61 CBV.dial :waste1 @14:07:18.91 delay 5 seconds @14:07:23.93 CTV.dial :air,avoiding: :intake @14:07:25.35 Storing lyfil puck in Collection Clamp to tube 1 @14:07:25.50 SC.reconfigure :tight @14:07:25.55 SC.seek 1 @14:07:29.14 SC.reconfigure :loose @14:07:29.33 Elbow.reconfigure :tight @14:07:29.37 Elbow.seek 1 @14:07:31.16 Forearm.reconfigure :tight @14:07:31.20 Forearm.seek 1 + 450 counts @14:07:32.23 SE.reconfigure :pick @14:07:32.27 SE.seek :beamBreak @14:07:35.30 SE.configure :default @14:07:35.44 Elbow.reconfigure :limp @14:07:35.49 Forearm.reconfigure :loose @14:07:35.53 Forearm.seek 1 @14:07:35.75 Elbow.hold @14:07:35.81 Elbow.reconfigure :tight @14:07:35.89 SE.seek 0.15 Pucks below Top Plate @14:07:36.30 Forearm.force -20 @14:07:36.34 Forearm.seek 1 + 315 counts @14:07:36.63 Forearm.reconfigure :tight @14:07:36.67 Forearm.seek 1 + 450 counts @14:07:36.86 SE.seek 1.15 Pucks below Top Plate @14:07:37.37 Forearm.reconfigure :loose @14:07:37.41 Forearm.seek :retract @14:07:38.39 Elbow.seek CC @14:07:40.10 Elbow.reconfigure :loose @14:07:40.19 Forearm.seek CC @14:07:41.16 Hand.close @14:07:41.86 CC.seek :open @14:07:46.61 Forearm.seek :retract @14:07:47.59 Elbow.reconfigure :tight @14:07:47.63 Elbow.seek 1 @14:07:49.41 Elbow.reconfigure :limp @14:07:49.46 Forearm.seek 1 @14:07:50.48 Elbow.hold @14:07:50.54 Elbow.reconfigure :tight @14:07:50.58 Hand.open @14:07:51.34 Forearm.force -20 @14:07:51.37 Forearm.seek 1 + 319 counts @14:07:51.69 Forearm.reconfigure :tight @14:07:51.73 Forearm.seek 1 + 450 counts @14:07:52.47 SE.seek :down @14:07:53.59
Forearm.reconfigure :loose @14:07:53.63 Forearm.seek :retract @14:07:55.16 SE.coast @14:07:55.20
SC.coast @14:07:55.23 PSV.dial :air @14:07:55.99 PS.seek 2.5ml @14:08:22.41 delay 1 second @14:08:23.43 PSV.dial :mixloop @14:08:23.88 delay 1 second @14:08:24.90 PSV.dial :air @14:08:25.36 delay 1 second @14:08:26.38 PSV.dial :mixloop @14:08:26.85 delay 1 second @14:08:27.87 PSV.dial :air @14:08:28.33 delay 1 second @14:08:29.35 PS.seek 0ml @14:08:55.75 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:08:55.92 Elbow.seek FlushPuck::Garage @14:08:57.78 Elbow.reconfigure :loose @14:08:57.87 Forearm.seek :garage @14:08:58.84 Hand.close @14:08:59.59 Forearm.seek :retract @14:09:00.56 Elbow.reconfigure :tight @14:09:00.60 Elbow.seek PC @14:09:01.85 Elbow.reconfigure :loose @14:09:01.94 Forearm.reconfigure :tight @14:09:01.98 Forearm.seek PC @14:09:03.17 PC.seek :closed @14:09:07.80 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.64 @14:09:07.86 Hand.open @14:09:08.53 Forearm.reconfigure :loose @14:09:08.57 Forearm.seek :retract @14:09:09.53 PRV.dial :air @14:09:09.97 PSV.dial :mixloop @14:09:10.50 PS.seek 0.3ml @14:09:14.37 PSV.dial PSR @14:09:15.47 Pulling 0.4ml of PSR.air @14:09:15.48 PSR.select :air @14:09:15.50 PS.seek 0.7ml @14:09:20.44 delay 3 seconds @14:09:23.46 PSR.select :air @14:09:23.48 PS.seek 2.5ml @14:09:42.75 delay 3 seconds @14:09:45.77 PSV.dial :mixloop @14:09:46.94 PTV.dial :mixing @14:09:47.69 PBV.dial :waste2 @14:09:48.63 PS.seek 0ml @14:10:15.22 Garaging FlushPuck in Processing Clamp @14:10:15.28 Elbow.reconfigure :tight @14:10:15.32 Elbow.seek PC @14:10:15.72 Elbow.reconfigure :loose @14:10:15.81 Forearm.seek PC @14:10:16.78 Hand.close @14:10:17.36 PC.seek :open @14:10:22.07 Forearm.seek :retract @14:10:23.03 Elbow.reconfigure :tight @14:10:23.07 Elbow.seek :garage @14:10:24.38 Elbow.reconfigure :loose @14:10:24.47 Forearm.reconfigure :tight @14:10:24.51 Forearm.seek :garage @14:10:25.53 Hand.open @14:10:26.17 Forearm.reconfigure :loose @14:10:26.21 Forearm.seek :retract @14:10:27.20 Loading da puck from tube 2 into Processing Clamp @14:10:27.41 SC.reconfigure :tight @14:10:27.45 SC.seek 2 @14:10:31.00 SC.reconfigure :loose @14:10:31.18 Elbow.reconfigure :tight @14:10:31.22 Elbow.seek 2 @14:10:33.07 Forearm.reconfigure :tight @14:10:33.11 Forearm.seek 2 + 450 counts @14:10:34.38 SE.reconfigure :pick @14:10:34.43 SE.seek :beamBreak @14:10:37.49 SE.configure :default @14:10:37.63 Elbow.seek 2 @14:10:38.53 Forearm.seek 2 + 300 counts @14:10:38.77 Forearm.reconfigure :loose @14:10:38.81 Hand.close @14:10:39.50 Puck::Warning in MAIN -- Loaded Last remaining Puck (from Tube 2) @14:10:39.51 SE.seek :down @14:10:39.69
Forearm.seek :retract @14:10:40.64 Elbow.seek PC @14:10:42.31 SE.coast @14:10:43.14
Elbow.reconfigure :loose @14:10:43.23 Forearm.reconfigure :tight @14:10:43.27 Forearm.seek PC @14:10:44.57 PC.seek :closed @14:10:49.18 Processing Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.65 @14:10:49.19 Hand.open @14:10:49.91 Forearm.reconfigure :loose @14:10:49.96 Forearm.seek :retract @14:10:50.91 SC.coast @14:10:50.95 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:10:50.96 PH.seek 28C,5:00 @14:11:35.20 PH.hold 2:00:00,28C @14:11:35.25 delay 1:30 @14:13:05.29 PSR.select :air @14:13:05.30 PSV.dial PSR @14:13:06.51 Pulling 0.5ml of PSR.wash @14:13:06.52 PSR.select :wash @14:13:06.63 PS.seek 0.5ml @14:13:12.56 delay 5 seconds @14:13:17.58 PSR.select :air @14:13:17.71 PS.seek 2ml @14:13:33.91 PTV.dial :puck @14:13:34.64 PSV.dial PTV @14:13:35.50 PBV.dial :waste1 @14:13:36.89 PS.seek 1.5ml @14:13:42.85 delay 3 seconds @14:13:45.87 PBV.dial between PSV and waste1 @14:13:46.22 PS.seek 0ml @14:14:02.53 delay 5:00 @14:19:02.55 recoverPSRreagent08 @14:19:02.56 PTV.dial :puck @14:19:02.57 PBV.dial :waste1 @14:19:02.92 delay 30 seconds @14:19:32.95 PBV.dial between PSV and waste1 @14:19:33.32 PSR.select :air @14:19:33.33 PSV.dial PSR @14:19:34.08 PS.reconfigure :fast @14:19:34.12 PS.seek 2.5ml @14:19:58.16 PSV.dial PTV @14:19:58.92 delay 5 seconds @14:20:03.94 PSV.dial PSR @14:20:04.69 delay 5 seconds @14:20:09.71 PSV.dial PTV @14:20:10.47 delay 5 seconds @14:20:15.49 PSV.dial :air @14:20:16.25 PS.seek 0ml @14:20:39.78 PTV.dial :PRVmixing,via: PRV @14:20:41.11 PSV.dial :mixloop @14:20:41.60 PRV.dial :ab1 @14:20:42.35 PS.reconfigure :slow @14:20:42.39 PS.seek 0.15ml @14:20:46.00 delay 2 seconds @14:20:48.02 CBV.dial PRV @14:20:48.05
PRV.dial CBV @14:20:48.61 PS.seek 0.36ml @14:20:53.38 delay 2 seconds @14:20:55.40 CBV.dial between PRV and PSV @14:20:55.71 PRV.dial :air @14:20:56.16 PS.seek 0.9ml @14:21:07.66 PRV.dial :waste2 @14:21:08.39 PS.seek 0.5ml @14:21:17.07 addPMLReagent08 @14:21:17.08 PSV.dial :air @14:21:17.55 PS.reconfigure :default @14:21:17.59 PS.seek 2ml @14:21:33.81 PSV.dial :PML @14:21:34.74 PTV.dial :mixing @14:21:35.49 PBV.dial :waste1 @14:21:35.91 PS.seek 1.85ml @14:21:38.28 PBV.dial between PSV and waste1 @14:21:38.64 PS.seek 0ml @14:21:58.41 delay 2 seconds @14:22:00.43 Beginning samplePassC @14:22:00.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:22:00.60 Elbow.reconfigure :tight @14:22:00.65 Elbow.seek FlushPuck::Garage @14:22:01.99 Elbow.reconfigure :loose @14:22:02.08 Forearm.seek :garage @14:22:03.10 Hand.close @14:22:03.98 Forearm.seek :retract @14:22:04.96 Elbow.reconfigure :tight @14:22:05.00 Elbow.seek CC @14:22:07.85 Elbow.reconfigure :loose @14:22:07.94 Forearm.reconfigure :tight @14:22:07.98 Forearm.seek CC @14:22:09.23 CC.seek :closed @14:22:13.77 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @14:22:13.83 Hand.open @14:22:14.55 Forearm.reconfigure :loose @14:22:14.59 Forearm.seek :retract @14:22:15.55 PSV.dial PSR @14:22:16.77 Pulling 1.5ml of PSR.flush @14:22:16.78 PSR.select :flush @14:22:16.90 PS.seek 1.5ml @14:22:33.03 delay 5 seconds @14:22:38.05 PSV.dial :waste1 @14:22:38.52 PS.seek 0ml @14:22:54.66 PSV.dial PSR @14:22:55.13 PSR.select :air @14:22:55.24 PS.seek 2.5ml @14:23:21.60 PRV.dial CBV,avoiding: :ab2 @14:23:21.63
CBV.dial PRV @14:23:23.25 PSV.dial :mixloop @14:23:24.30 CTV.dial :air,avoiding: :intake @14:23:24.31 PTV.dial :PRVmixing @14:23:25.08 PS.seek 0ml @14:23:51.46 PSV.dial :air @14:23:52.07 PS.seek 2.5ml @14:24:18.47 PSV.dial :mixloop @14:24:18.92 PS.seek 0ml @14:24:45.32 PSV.dial :air @14:24:45.78 PS.seek 2.5ml @14:25:12.21 PSV.dial :mixloop @14:25:12.69 PS.seek 0ml @14:25:39.00 Garaging FlushPuck in Collection Clamp @14:25:39.06 Elbow.reconfigure :tight @14:25:39.11 Elbow.seek CC @14:25:39.40 Elbow.reconfigure :loose @14:25:39.48 Forearm.seek CC @14:25:40.46 Hand.close @14:25:41.24 CC.seek :open @14:25:45.88 Forearm.seek :retract @14:25:46.85 Elbow.reconfigure :tight @14:25:46.89 Elbow.seek :garage @14:25:49.82 Elbow.reconfigure :loose @14:25:49.91 Forearm.reconfigure :tight @14:25:49.95 Forearm.seek :garage @14:25:50.93 Hand.open @14:25:51.54 Forearm.reconfigure :loose @14:25:51.58 Forearm.seek :retract @14:25:52.60 delayUntil "14:30:00.41" @14:30:00.43 recoverPRVreagent08 @14:30:00.44 PTV.dial :mixing @14:30:01.21 PBV.dial :waste1 @14:30:01.58 delay 30 seconds @14:30:31.61 PBV.dial between PSV and waste1 @14:30:31.99 PSV.dial :air @14:30:32.46 PS.reconfigure :fast @14:30:32.50 PS.seek 2.5ml @14:30:56.46 PSV.dial :mixloop @14:30:56.94 delay 2 seconds @14:30:58.96 PSV.dial :air @14:30:59.43 delay 2 seconds @14:31:01.45 PSV.dial :mixloop @14:31:02.38 delay 2 seconds @14:31:04.40 PSV.dial :air @14:31:04.86 PS.seek 0ml @14:31:28.35 washCycleDA #1 of 2 @14:31:28.36 PSR.select :air @14:31:28.37 PSV.dial PSR @14:31:29.76 Pulling 0.5ml of PSR.wash @14:31:29.77 PSR.select :wash @14:31:29.89 PS.reconfigure :default @14:31:29.93 PS.seek 0.5ml @14:31:35.82 delay 5 seconds @14:31:40.84 PSR.select :air @14:31:40.96 PS.seek 2ml @14:31:57.15 PTV.dial :puck @14:31:57.89 PSV.dial PTV @14:31:58.75 PBV.dial :waste1 @14:31:59.18 PS.seek 1.5ml @14:32:05.16 delay 3 seconds @14:32:08.18 PBV.dial between PSV and waste1 @14:32:08.55 PS.seek 0ml @14:32:24.63 delay 2:00 @14:34:24.66 recoverPSRreagent08 @14:34:24.67 PTV.dial :puck @14:34:24.68 PBV.dial :waste1 @14:34:25.05 delay 30 seconds @14:34:55.08 PBV.dial between PSV and waste1 @14:34:55.46 PSR.select :air @14:34:55.47 PSV.dial PSR @14:34:56.22 PS.reconfigure :fast @14:34:56.26 PS.seek 2.5ml @14:35:20.43 PSV.dial PTV @14:35:21.21 delay 5 seconds @14:35:26.23 PSV.dial PSR @14:35:26.99 delay 5 seconds @14:35:32.01 PSV.dial PTV @14:35:32.77 delay 5 seconds @14:35:37.79 PSV.dial :air @14:35:38.66 PS.seek 0ml @14:36:02.13 washCycleDA #2 of 2 @14:36:02.14 PSR.select :air @14:36:02.15 PSV.dial PSR @14:36:03.52 Pulling 0.5ml of PSR.wash @14:36:03.53 PSR.select :wash @14:36:03.64 PS.reconfigure :default @14:36:03.68 PS.seek 0.5ml @14:36:09.63 delay 5 seconds @14:36:14.65 PSR.select :air @14:36:14.79 PS.seek 2ml @14:36:30.93 PTV.dial :puck @14:36:30.95 PSV.dial PTV @14:36:31.71 PBV.dial :waste1 @14:36:32.13 PS.seek 1.5ml @14:36:38.10 delay 3 seconds @14:36:41.12 PBV.dial between PSV and waste1 @14:36:41.49 PS.seek 0ml @14:36:57.66 delay 2:00 @14:38:57.69 recoverPSRreagent08 @14:38:57.70 PTV.dial :puck @14:38:57.71 PBV.dial :waste1 @14:38:58.08 delay 30 seconds @14:39:28.11 PBV.dial between PSV and waste1 @14:39:28.49 PSR.select :air @14:39:28.50 PSV.dial PSR @14:39:29.27 PS.reconfigure :fast @14:39:29.31 PS.seek 2.5ml @14:39:53.24 PSV.dial PTV @14:39:54.02 delay 5 seconds @14:39:59.04 PSV.dial PSR @14:39:59.79 delay 5 seconds @14:40:04.81 PSV.dial PTV @14:40:05.68 delay 5 seconds @14:40:10.70 PSV.dial :air @14:40:11.47 PS.seek 0ml @14:40:34.94 getPMLReagent08 @14:40:34.95 PRV.dial :air @14:40:35.41 delay 5 seconds @14:40:40.43 PTV.dial :PRVmixing @14:40:41.77 delay 5 seconds @14:40:46.79 PRV.dial :ab2 @14:40:47.24 PTV.dial :PRVmixing @14:40:47.25 PSV.dial :mixloop @14:40:47.72 PS.reconfigure :default @14:40:47.76 PS.seek 0.3ml @14:40:51.75 delay 2 seconds @14:40:53.77 PRV.dial :air @14:40:54.29 PS.seek 0.55ml @14:40:57.66 addPMLReagent08 @14:40:57.67 PSV.dial :air @14:40:58.13 PS.seek 2ml @14:41:13.74 PSV.dial :PML @14:41:14.21 PTV.dial :mixing @14:41:14.96 PBV.dial :waste1 @14:41:15.38 PS.seek 1.85ml @14:41:17.68 PBV.dial between PSV and waste1 @14:41:18.07 PS.seek 0ml @14:41:37.82 delay 2 seconds @14:41:39.84 delay 8:00 @14:49:39.86 recoverPRVreagent08 @14:49:39.87 PTV.dial :mixing @14:49:39.88 PBV.dial :waste1 @14:49:40.26 delay 30 seconds @14:50:10.29 PBV.dial between PSV and waste1 @14:50:10.66 PSV.dial :air @14:50:11.13 PS.reconfigure :fast @14:50:11.17 PS.seek 2.5ml @14:50:35.31 PSV.dial :mixloop @14:50:35.79 delay 2 seconds @14:50:37.81 PSV.dial :air @14:50:38.27 delay 2 seconds @14:50:40.29 PSV.dial :mixloop @14:50:40.77 delay 2 seconds @14:50:42.79 PSV.dial :air @14:50:43.26 PS.seek 0ml @14:51:06.82 washCycleDA #1 of 3 @14:51:06.83 PSR.select :air @14:51:06.84 PSV.dial PSR @14:51:08.23 Pulling 0.5ml of PSR.wash @14:51:08.24 PSR.select :wash @14:51:08.35 PS.reconfigure :default @14:51:08.39 PS.seek 0.5ml @14:51:14.41 delay 5 seconds @14:51:19.43 PSR.select :air @14:51:19.55 PS.seek 2ml @14:51:35.75 PTV.dial :puck @14:51:36.49 PSV.dial PTV @14:51:37.80 PBV.dial :waste1 @14:51:38.23 PS.seek 1.5ml @14:51:44.26 delay 3 seconds @14:51:47.28 PBV.dial between PSV and waste1 @14:51:47.66 PS.seek 0ml @14:52:03.82 delay 2:00 @14:54:03.85 recoverPSRreagent08 @14:54:03.86 PTV.dial :puck @14:54:03.87 PBV.dial :waste1 @14:54:04.24 delay 30 seconds @14:54:34.27 PBV.dial between PSV and waste1 @14:54:34.63 PSR.select :air @14:54:34.65 PSV.dial PSR @14:54:35.40 PS.reconfigure :fast @14:54:35.45 PS.seek 2.5ml @14:54:59.41 PSV.dial PTV @14:55:00.29 delay 5 seconds @14:55:05.31 PSV.dial PSR @14:55:06.04 delay 5 seconds @14:55:11.06 PSV.dial PTV @14:55:11.93 delay 5 seconds @14:55:16.95 PSV.dial :air @14:55:17.83 PS.seek 0ml @14:55:41.35 washCycleDA #2 of 3 @14:55:41.36 PSR.select :air @14:55:41.37 PSV.dial PSR @14:55:42.74 Pulling 0.5ml of PSR.wash @14:55:42.75 PSR.select :wash @14:55:42.86 PS.reconfigure :default @14:55:42.90 PS.seek 0.5ml @14:55:48.88 delay 5 seconds @14:55:53.90 PSR.select :air @14:55:54.02 PS.seek 2ml @14:56:10.22 PTV.dial :puck @14:56:10.23 PSV.dial PTV @14:56:10.99 PBV.dial :waste1 @14:56:11.40 PS.seek 1.5ml @14:56:17.41 delay 3 seconds @14:56:20.43 PBV.dial between PSV and waste1 @14:56:20.79 PS.seek 0ml @14:56:36.94 delay 2:00 @14:58:36.97 recoverPSRreagent08 @14:58:36.98 PTV.dial :puck @14:58:36.99 PBV.dial :waste1 @14:58:37.35 delay 30 seconds @14:59:07.38 PBV.dial between PSV and waste1 @14:59:07.74 PSR.select :air @14:59:07.75 PSV.dial PSR @14:59:08.51 PS.reconfigure :fast @14:59:08.55 PS.seek 2.5ml @14:59:32.40 PSV.dial PTV @14:59:33.16 delay 5 seconds @14:59:38.18 PSV.dial PSR @14:59:38.93 delay 5 seconds @14:59:43.95 PSV.dial PTV @14:59:44.82 delay 5 seconds @14:59:49.84 PSV.dial :air @14:59:50.60 PS.seek 0ml @15:00:14.07 washCycleDA #3 of 3 @15:00:14.08 PSR.select :air @15:00:14.09 PSV.dial PSR @15:00:15.48 Pulling 0.5ml of PSR.wash @15:00:15.49 PSR.select :wash @15:00:15.60 PS.reconfigure :default @15:00:15.64 PS.seek 0.5ml @15:00:21.66 delay 5 seconds @15:00:26.68 PSR.select :air @15:00:26.83 PS.seek 2ml @15:00:42.96 PTV.dial :puck @15:00:42.98 PSV.dial PTV @15:00:43.71 PBV.dial :waste1 @15:00:44.12 PS.seek 1.5ml @15:00:50.04 delay 3 seconds @15:00:53.06 PBV.dial between PSV and waste1 @15:00:53.41 PS.seek 0ml @15:01:09.51 delay 2:00 @15:03:09.54 recoverPSRreagent08 @15:03:09.55 PTV.dial :puck @15:03:09.56 PBV.dial :waste1 @15:03:09.91 delay 30 seconds @15:03:39.94 PBV.dial between PSV and waste1 @15:03:40.30 PSR.select :air @15:03:40.31 PSV.dial PSR @15:03:41.07 PS.reconfigure :fast @15:03:41.11 PS.seek 2.5ml @15:04:04.97 PSV.dial PTV @15:04:05.83 delay 5 seconds @15:04:10.85 PSV.dial PSR @15:04:11.60 delay 5 seconds @15:04:16.62 PSV.dial PTV @15:04:17.38 delay 5 seconds @15:04:22.40 PSV.dial :air @15:04:23.15 PS.seek 0ml @15:04:46.69 Gate.power :camera,:ON @15:04:46.70 Mixing DA Substrates @15:04:46.71 PSR.select :air @15:04:46.72 PSV.dial PSR @15:04:48.30 Pulling 0.25ml of PSR.sub1 @15:04:48.32 PSR.select :sub1 @15:04:48.48 PS.reconfigure :default @15:04:48.57 PS.seek 0.25ml @15:04:52.04 delay 5 seconds @15:04:57.11 Pulling 0.25ml of PSR.sub2 @15:04:57.12 PSR.select :sub2 @15:04:57.24 PS.seek 0.5ml @15:05:00.66 delay 5 seconds @15:05:05.68 PSR.select :air @15:05:05.79 PS.seek 2ml @15:05:21.93 PTV.dial :puck @15:05:21.95 PSV.dial PTV @15:05:22.72 PBV.dial :waste1 @15:05:23.15 PS.seek 1.5ml @15:05:29.07 delay 3 seconds @15:05:32.09 PBV.dial between PSV and waste1 @15:05:32.46 PS.seek 0ml @15:05:48.63 delay 10 seconds @15:05:58.66 recoverPSRreagent08 @15:05:58.67 PTV.dial :puck @15:05:58.68 PBV.dial :waste1 @15:05:59.05 delay 30 seconds @15:06:29.08 PBV.dial between PSV and waste1 @15:06:29.47 PSR.select :air @15:06:29.48 PSV.dial PSR @15:06:30.27 PS.reconfigure :fast @15:06:30.32 PS.seek 2.5ml @15:06:55.43 PSV.dial PTV @15:06:56.69 delay 5 seconds @15:07:01.71 PSV.dial PSR @15:07:02.51 delay 5 seconds @15:07:07.53 PSV.dial PTV @15:07:08.32 delay 5 seconds @15:07:13.34 PSV.dial :air @15:07:14.10 PS.seek 0ml @15:07:38.69 Additional air clear @15:07:38.70 PSR.select :air @15:07:38.71 PSV.dial PSR @15:07:40.12 Pulling 2ml of PSR.air @15:07:40.13 PSR.select :air @15:07:40.15 PS.reconfigure :default @15:07:40.19 PS.seek 2ml @15:08:01.51 delay 5 seconds @15:08:06.53 PTV.dial :puck @15:08:06.55 PSV.dial PTV @15:08:07.33 PBV.dial :waste1 @15:08:07.76 PS.seek 1.5ml @15:08:13.69 delay 3 seconds @15:08:16.71 PBV.dial between PSV and waste1 @15:08:17.08 delay 1 second @15:08:18.10 PSV.dial :waste1 @15:08:19.18 PS.seek 0ml @15:08:35.42 Processing Puck Heater at 28.1C before imaging @15:08:35.43 PH.coast @15:08:35.55 Imaging da puck in Processing Clamp while storing it to tube 1 @15:08:35.70 SC.reconfigure :tight @15:08:35.74 SC.seek 1 @15:08:39.27 SC.reconfigure :loose @15:08:39.45 Elbow.reconfigure :tight @15:08:39.49 Elbow.seek 1 @15:08:41.38 Forearm.reconfigure :tight @15:08:41.43 Forearm.seek 1 + 450 counts @15:08:42.51 SE.reconfigure :pick @15:08:42.56 SE.seek :beamBreak @15:08:45.61 SE.configure :default @15:08:45.75 Elbow.reconfigure :limp @15:08:45.80 Forearm.reconfigure :loose @15:08:45.84 Forearm.seek 1 @15:08:46.07 Elbow.hold @15:08:46.13 Elbow.reconfigure :tight @15:08:46.21 SE.seek 1.15 Pucks below Top Plate @15:08:46.72 Forearm.force -20 @15:08:46.75 Forearm.seek 1 + 319 counts @15:08:47.04 Forearm.reconfigure :tight @15:08:47.08 Forearm.seek 1 + 450 counts @15:08:47.29 SE.seek 2.15 Pucks below Top Plate @15:08:47.94 Forearm.reconfigure :loose @15:08:47.98 Forearm.seek :retract @15:08:48.92 Elbow.seek PC @15:08:51.47 Elbow.reconfigure :loose @15:08:51.56 Forearm.seek PC @15:08:52.50 Hand.close @15:08:53.26 PC.seek :open @15:08:58.00 Forearm.seek :retract @15:08:59.00 Elbow.reconfigure :tight @15:08:59.04 Elbow.seek 1 @15:09:01.64 Forearm.reconfigure :tight @15:09:01.69 Forearm.seek 1 + 450 counts @15:09:02.67 Hand.open @15:09:03.45 SE.seek 1.8 Pucks below Top Plate @15:09:03.74 SE.reconfigure :seal @15:09:03.78 SE.seek 0.84 Pucks below Top Plate @15:09:04.33 Lights, camera, ACTION! @15:09:04.36 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21mar1015h100ml.tif @15:09:23.84 Waiting >20 seconds for da to relinquish FlushPuck @15:09:26.43 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 18.86 seconds /var/log/esp/da21mar1015h100ml.tif: TIFF Upload Complete @15:09:26.46 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21mar1015h100ml15s.tif @15:09:42.02 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21mar1015h100ml15s.tif: TIFF Upload Complete @15:09:42.05 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21mar1015h100ml30s.tif @15:10:12.61 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21mar1015h100ml30s.tif: TIFF Upload Complete @15:10:12.64 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21mar1015h100ml70s.tif @15:11:24.20 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21mar1015h100ml70s.tif: TIFF Upload Complete @15:11:24.22 Gate.power :camera,:OFF @15:11:24.25 DAfans.email "18.86 sec auto-exposure",Subject: "da21mar1015h100ml images!" @15:11:24.46 Elbow.seek 1 @15:11:24.75 Forearm.seek 1 + 450 counts @15:11:24.83 SE.reconfigure :gently @15:11:24.88 SE.seek 1.55 Pucks below Top Plate @15:11:25.48 Elbow.reconfigure :limp @15:11:25.53 Forearm.reconfigure :loose @15:11:25.57 Forearm.seek 1 @15:11:25.81 Elbow.hold @15:11:25.88 Elbow.reconfigure :tight @15:11:25.93 SE.reconfigure :default @15:11:25.98 SE.seek 1.74 Pucks below Top Plate @15:11:27.42 Forearm.force -20 @15:11:27.45 Forearm.seek 1 + 316 counts @15:11:27.76 Forearm.reconfigure :tight @15:11:27.80 Forearm.seek 1 + 450 counts @15:11:28.14 SE.seek :down @15:11:29.27 Forearm.reconfigure :loose @15:11:29.32 Forearm.seek :retract @15:11:30.75 SE.coast @15:11:30.79 SC.coast @15:11:30.88 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:11:30.90
Starting finalPurge @15:11:31.08 Elbow.seek FlushPuck::Garage @15:11:33.02 Elbow.reconfigure :loose @15:11:33.11 Forearm.seek :garage @15:11:34.08 Hand.close @15:11:34.86 Forearm.seek :retract @15:11:35.84 Elbow.reconfigure :tight @15:11:35.88 Elbow.seek PC @15:11:37.16 Elbow.reconfigure :loose @15:11:37.25 Forearm.reconfigure :tight @15:11:37.29 Forearm.seek PC @15:11:38.60 PC.seek :closed @15:11:43.22 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.65 @15:11:43.28 Hand.open @15:11:43.94 Forearm.reconfigure :loose @15:11:43.98 Forearm.seek :retract @15:11:44.93 PSR.select :air @15:11:44.95 PSV.dial PSR @15:11:45.48 Pulling 0.5ml of PSR.flush @15:11:45.49 PSR.select :flush @15:11:45.61 PS.seek 0.5ml @15:11:50.76
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:11:51.51 delay 5 seconds @15:11:56.53 PSR.select :air @15:11:56.64 PS.seek 2.5ml @15:12:17.91 delay 1 second @15:12:18.93 PRV.dial :waste2,avoiding: :ab2 @15:12:20.79 PTV.dial :PRVmixing @15:12:22.10 PSV.dial :mixloop @15:12:23.16 PS.seek :empty @15:12:49.50 PSR.select :air @15:12:49.52 PSV.dial PSR @15:12:50.62 Pulling 0.5ml of PSR.flush @15:12:50.63 PSR.select :flush @15:12:50.74 PS.seek 0.5ml @15:12:56.76 delay 5 seconds @15:13:01.78 PSR.select :air @15:13:01.89 PS.seek 2.5ml @15:13:23.07 delay 1 second @15:13:24.09 PBV.dial :waste1 @15:13:24.44 PTV.dial :mixing @15:13:25.21 PSV.dial :mixloop @15:13:26.26 PS.seek :empty @15:13:52.57 PSV.dial PSR @15:13:53.62 PSR.select :flush @15:13:53.74 PS.seek 1ml @15:14:04.90 PSR.select :air @15:14:05.02 PS.seek 2.5ml @15:14:21.19 PSV.dial PTV @15:14:21.94 PTV.dial :puck @15:14:22.68 PBV.dial :waste1 @15:14:22.69 PS.seek 0ml @15:14:49.06 PSV.dial PSR @15:14:49.82 PSR.select :flush @15:14:49.94 PS.seek 0.5ml @15:14:55.93 PSR.select :air @15:14:56.05 PS.seek 2.5ml @15:15:17.28 PSV.dial PTV @15:15:18.04 PBV.dial PSV @15:15:18.60 PS.seek 0ml @15:15:44.87 PSV.dial PBV @15:15:45.35 PS.seek 2.5ml @15:16:11.66 PSV.dial :waste1 @15:16:12.40 PS.seek 0ml @15:16:38.78 PSV.dial PSR @15:16:39.26 PSR.select :air @15:16:39.28 PS.seek 2.5ml @15:17:05.87 PSV.dial PTV @15:17:06.62 PBV.dial PSV @15:17:07.09 PS.seek 0ml @15:17:33.62 PSV.dial PBV @15:17:34.10 PS.seek 2.5ml @15:18:00.53 PSV.dial :waste1 @15:18:01.27 PS.seek 0ml @15:18:27.60 PSV.dial PSR @15:18:28.07 PSR.select :flush @15:18:28.19 PS.seek 1ml @15:18:39.18 PSR.select :air @15:18:39.30 PS.seek 2.5ml @15:18:55.47 PSV.dial PTV @15:18:56.21 PTV.dial :puck @15:18:56.22 PBV.dial :waste1 @15:18:56.79 PS.seek 0ml @15:19:23.12 PSV.dial PSR @15:19:23.88 PSR.select :flush @15:19:24.00 PS.seek 0.5ml @15:19:29.90 PSR.select :air @15:19:30.02 PS.seek 2.5ml @15:19:51.23 PSV.dial PTV @15:19:51.98 PBV.dial PSV @15:19:52.52 PS.seek 0ml @15:20:18.90 PSV.dial PBV @15:20:19.38 PS.seek 2.5ml @15:20:45.75 PSV.dial :waste1 @15:20:46.49 PS.seek 0ml @15:21:12.81 PSV.dial PSR @15:21:13.29 PSR.select :air @15:21:13.31 PS.seek 2.5ml @15:21:39.69 PSV.dial PTV @15:21:40.43 PBV.dial PSV @15:21:40.44 PS.seek 0ml @15:22:06.81 PSV.dial PBV @15:22:07.29 PS.seek 2.5ml @15:22:33.66 PSV.dial :waste1 @15:22:34.40 PS.seek 0ml @15:23:00.76
Loading FlushPuck into Collection Clamp from Processing Clamp @15:23:00.88 Elbow.reconfigure :tight @15:23:00.92 Elbow.seek PC @15:23:01.03 Elbow.reconfigure :loose @15:23:01.11 Forearm.seek PC @15:23:02.05 Hand.close @15:23:02.77 PC.seek :open @15:23:07.51 Forearm.seek :retract @15:23:08.46 Elbow.reconfigure :tight @15:23:08.50 Elbow.seek CC @15:23:12.13 Elbow.reconfigure :loose @15:23:12.21 Forearm.reconfigure :tight @15:23:12.25 Forearm.seek CC @15:23:13.55 CC.seek :closed @15:23:18.06 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.31 @15:23:18.12 Hand.open @15:23:18.83 Forearm.reconfigure :loose @15:23:18.87 Forearm.seek :retract @15:23:19.83 Flushing the Collection Valve::Manifold @15:23:19.94 CSV.dial CSR @15:23:20.45 Pulling 5ml of CSR.flush @15:23:20.46 CSR.select :flush @15:23:20.57 CS.seek 5ml @15:23:34.39 delay 5 seconds @15:23:39.41 CSR.select :air @15:23:39.57 CS.reconfigure :fast @15:23:39.61 CS.seek 7.501ml @15:23:46.47 CSV.dial :waste @15:23:47.78 CS.reconfigure :default @15:23:47.82 CS.seek 0ml @15:24:07.89 Flushing lower manifold's waste1 port @15:24:08.00 CSV.dial :flush @15:24:08.45 CS.reconfigure :fast @15:24:08.49 CS.seek 5ml @15:24:21.54 delay 5 seconds @15:24:26.57 CSV.dial :air @15:24:27.20 CS.seek 7.001ml @15:24:32.98 CSV.dial CTV @15:24:33.01
CTV.dial CSV @15:24:34.42 CBV.dial :waste1 @15:24:34.87 CS.seek 0ml @15:24:51.76 Flushing lower manifold's waste2 port @15:24:52.30 CSV.dial :flush @15:24:53.18 CS.seek 5ml @15:25:06.26 delay 5 seconds @15:25:11.28 CSV.dial :air @15:25:11.79 CS.seek 7ml @15:25:17.64 CSV.dial CTV @15:25:17.68
CTV.dial CSV @15:25:18.09 CBV.dial :waste2 @15:25:19.14 CS.seek 0ml @15:25:36.04 Disinfecting external Intake with kill @15:25:36.15 CSV.dial CSR @15:25:36.76 Pulling 8ml of CSR.kill @15:25:36.77 CSR.select :kill @15:25:36.88 CS.seek 8ml @15:25:56.98 delay 5 seconds @15:26:02.00 CSR.select :air @15:26:02.12 CS.seek 10ml @15:26:07.77 CSV.dial CBV @15:26:07.80
CBV.dial CSV @15:26:09.18 CTV.dial :intake @15:26:09.96 Intake.select :open @15:26:10.07 CS.seek 2ml @15:26:29.25 Intake.select :close @15:26:29.36 CTV.dial :air @15:26:30.14 CS.reconfigure :default @15:26:30.18 CS.seek 0ml @15:26:36.13 CSV.dial CSR @15:26:37.33 Pulling 5ml of CSR.flush @15:26:37.34 CSR.select :flush @15:26:37.45 CS.seek 5ml @15:26:51.42 delay 5 seconds @15:26:56.44 CSR.select :air @15:26:56.56 CS.seek 10ml @15:27:10.20 CSV.dial CBV @15:27:11.20 CS.seek 0ml @15:27:37.64 Garaging FlushPuck in Collection Clamp @15:27:37.70 Elbow.reconfigure :tight @15:27:37.75 Elbow.seek CC @15:27:38.10 Elbow.reconfigure :loose @15:27:38.19 Forearm.seek CC @15:27:39.16 Hand.close @15:27:39.90 CC.seek :open @15:27:44.52 Forearm.seek :retract @15:27:45.51 Elbow.reconfigure :tight @15:27:45.55 Elbow.seek :garage @15:27:48.43 Elbow.reconfigure :loose @15:27:48.52 Forearm.reconfigure :tight @15:27:48.56 Forearm.seek :garage @15:27:49.58 Hand.open @15:27:50.20 Forearm.reconfigure :loose @15:27:50.24 Forearm.seek :retract @15:27:51.24 Completed [initialPurge; da #da and wsr are run simultanelously] begun 13:35:05 @15:27:51.28 Asleep after completing DA_03092021 assays @15:27:51.30 GoodNews.email "{1 => 3,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@15:24:15, 0% flow, 22.7C, 17% humidity, 15.2psia, 13.510V, 0.472A, 0.453A avg, 6.37W\nSampled 100.0ml",Subject: "Asleep after completing DA_03092021 assays" @07:39:52.68PDT27-Jul-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @07:39:55.26 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @07:39:58.92 Polling Can every 10:00 @07:39:58.97 Loading /home/esp/esp2/mission/seawater_negative_test_72721.rb mission @07:39:59.02 Preparing to sleep until + 00:00:05 @07:39:59.37 GoodNews.email "Starting seawater_negative_test_72721 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@07:39:58, 16% flow, 22.3C, 44% humidity, 14.9psia, 13.632V, 0.302A, 0.290A avg, 4.12W",Subject: "seawater_negative_test_72721 begun" @07:39:59.42
Asleep til + 00:00:05 @07:39:59.45 Continuing at 07:40 @07:39:59.71 Gate.power :raw,:OFF @07:39:59.72 Gate.power :core,:OFF @07:39:59.73 Shutdown @07:40:02.30 GoodNews.email "Then, in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/seawater_negative_test_72721.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@07:39:59, 16% flow, 22.2C, 44% humidity, 14.9psia, 13.627V, 0.302A, 0.290A avg, 4.12W",Subject: "Asleep until 07:40" @07:40:04.05
Powering core bus @07:40:09.71 Gate.power :raw,:ON @07:40:10.92 CS.configure :default @07:40:11.10 PS.configure :default @07:40:11.29 SS.configure :default @07:40:11.47 CC.configure :default @07:40:11.65 PC.configure :default @07:40:11.85 Forearm.configure :loose @07:40:12.02 Elbow.configure :loose @07:40:12.25 SC.configure :loose @07:40:12.42 SE.configure :default @07:40:12.49 CH.coast @07:40:12.60 CH.configure :default @07:40:12.66 PH.coast @07:40:12.77 PH.configure :default @07:40:12.83 All dwarf controllers running firmware version 3.85 @07:40:12.99 Valve.reset @07:40:13.38 Intake.select :close @07:40:13.42 Exhaust.select :close @07:40:13.50 CTV.dial :air @07:40:13.68 PSV.dial :air @07:40:13.75 CSV.dial :waste1 @07:40:13.98 SSV.dial :spit,avoiding: :exhaust @07:40:14.03
Forearm.reconfigure :loose @07:40:14.19 Forearm.home.seek :retract @07:40:14.39 CS.reconfigure :fast @07:40:14.46 SS.reconfigure :default @07:40:14.64 SS.home.seek 0ml @07:40:14.68 CS.home.seek 0ml @07:40:14.86 PS.reconfigure :default @07:40:14.96 PS.home.seek 0ml @07:40:15.07 Hand.close @07:40:15.14
Elbow.jog 1500 @07:40:15.74 Elbow.reconfigure :loose @07:40:15.82 Elbow.home.seek :clear @07:40:19.20 SE.reconfigure :default @07:40:19.24 SE.home.seek :down @07:40:19.89 SC.reconfigure :tight @07:40:19.94 SC.home.seek :initial @07:40:20.16 upsync @07:40:23.41 PS.stop @07:40:45.17
SC.configure :limp @07:40:45.22 Forearm.seek :retract @07:40:45.28 Elbow.seek :hammer @07:40:46.32 Forearm.reconfigure :tight @07:40:46.36 Forearm.seek retracted @07:40:46.61 PC.reconfigure :default @07:40:46.63
CC.reconfigure :default @07:40:46.71 CC.home.seek :open @07:40:46.73 PC.home.seek :open @07:40:50.23
Phase [initialPurge; da #da and wsr are run simultanelously] @07:40:50.25 Notice.email "At 07:40:50 began in tube 2: initialPurge; da #da and wsr are run simultanelously\n/home/esp/esp2/mission/seawater_negative_test_72721.rb:14\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@07:40:50, 16% flow, 22.3C, 43% humidity, 14.9psia, 13.588V, 0.393A, 0.375A avg, 5.34W",Subject: "Started seawater_negative_test_72721 phase initialPurge; da #da and wsr are run simultanelously" @07:40:50.27
Starting initialPurge @07:40:50.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @07:40:50.37 Hand.open @07:40:51.11 Elbow.reconfigure :tight @07:40:51.15 Elbow.seek FlushPuck::Garage @07:40:53.00 Elbow.reconfigure :loose @07:40:53.11 Forearm.reconfigure :loose @07:40:53.15 Forearm.seek :garage @07:40:54.20 Hand.close @07:40:55.04 Forearm.seek :retract @07:40:56.03 Elbow.reconfigure :tight @07:40:56.07 Elbow.seek CC @07:40:59.08 Elbow.reconfigure :loose @07:40:59.25 Forearm.reconfigure :tight @07:40:59.34 Forearm.seek CC @07:41:00.87 CC.seek :closed @07:41:05.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @07:41:05.67 Hand.open @07:41:06.38 Forearm.reconfigure :loose @07:41:06.46 Forearm.seek :retract @07:41:07.46 Flushing the Collection Valve::Manifold @07:41:07.70 CSV.dial CSR @07:41:09.13 Pulling 5ml of CSR.flush @07:41:09.14 CSR.select :flush @07:41:09.32 CS.reconfigure :default @07:41:09.41 CS.seek 5ml @07:41:10.74 upsync @07:41:23.21
delay 5 seconds @07:41:28.23 CSR.select :air @07:41:28.37 CS.reconfigure :fast @07:41:28.41 CS.seek 7.5ml @07:41:35.20 CSV.dial :waste @07:41:36.47 CS.reconfigure :default @07:41:36.51 CS.seek 0ml @07:41:56.46 Purging external Intake @07:41:56.57 CSV.dial :flush @07:41:57.01 CS.reconfigure :fast @07:41:57.05 CS.seek 10ml @07:42:21.42 delay 5 seconds @07:42:26.44 CSV.dial CBV @07:42:26.48
CBV.dial CSV @07:42:27.25 CTV.dial :intake @07:42:28.00 Intake.select :open @07:42:28.10 CS.seek 0ml @07:42:51.80 Intake.select :close @07:42:51.89 CTV.dial :air @07:42:52.69 Flushing upper manifold @07:42:52.80 CSV.dial :flush @07:42:53.54 CS.seek 5ml @07:43:06.39 delay 5 seconds @07:43:11.41 CSV.dial :air @07:43:12.03 CS.seek 7ml @07:43:17.52 CSV.dial CBV @07:43:17.54
CBV.dial CSV @07:43:18.55 CTV.dial :air @07:43:18.57 CS.seek 0ml @07:43:35.17 delay 7 seconds @07:43:42.20 CS.seek 3ml @07:43:50.36 CSV.dial :waste @07:43:50.80 CS.seek 0ml @07:43:58.65 CSV.dial :flush @07:43:59.08 CS.seek 3ml @07:44:07.05 delay 5 seconds @07:44:12.06 CSV.dial :air @07:44:12.68 CS.seek 5ml @07:44:18.20 CSV.dial :air @07:44:18.22 CS.seek 0ml @07:44:30.48 delay 7 seconds @07:44:37.51 CS.seek 3ml @07:44:45.51 CSV.dial :waste @07:44:46.22 CS.seek 0ml @07:44:54.02 Garaging FlushPuck in Collection Clamp @07:44:54.08 Elbow.reconfigure :tight @07:44:54.12 Elbow.seek CC @07:44:54.53 Elbow.reconfigure :loose @07:44:54.61 Forearm.seek CC @07:44:55.59 Hand.close @07:44:56.22 CC.seek :open @07:45:00.84 Forearm.seek :retract @07:45:01.86 Elbow.reconfigure :tight @07:45:01.91 Elbow.seek :garage @07:45:04.81 Elbow.reconfigure :loose @07:45:04.89 Forearm.reconfigure :tight @07:45:04.93 Forearm.seek :garage @07:45:05.97 Hand.open @07:45:06.65 Forearm.reconfigure :loose @07:45:07.13 Forearm.seek :retract @07:45:08.08 da sampling at most 100ml @07:45:08.11 DAfans.email "Started da in tube 2 at 07:45:08PDT27-Jul-21\n{1 => 0,\n 2 => 3,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@07:40:50, 16% flow, 22.3C, 43% humidity, 14.9psia, 13.588V, 0.393A, 0.375A avg, 5.34W",Subject: "da sampling at most 100ml" @07:45:08.14
Loading sh1 puck from tube 2 into Collection Clamp @07:45:08.37 SC.reconfigure :tight @07:45:08.43 SC.seek 2 @07:45:15.22 SC.configure :limp @07:45:15.66 Elbow.reconfigure :tight @07:45:15.74 Elbow.seek 2 @07:45:17.81 Forearm.reconfigure :tight @07:45:17.89 Forearm.seek 2 + 450 counts @07:45:19.06 SE.reconfigure :pick @07:45:19.14 SE.seek :beamBreak @07:45:22.20 SE.configure :default @07:45:22.50 Elbow.seek 2 @07:45:22.99 Forearm.seek 2 + 300 counts @07:45:23.23 Forearm.reconfigure :loose @07:45:23.30 Hand.close @07:45:24.02 SE.seek :down @07:45:24.42
Forearm.seek :retract @07:45:25.44 Elbow.seek CC @07:45:26.76 SE.stop @07:45:27.12 upsync @07:45:27.18
Elbow.reconfigure :loose @07:45:27.27 Forearm.reconfigure :tight @07:45:27.31 Forearm.seek CC @07:45:28.51 CC.seek :closed @07:45:33.02 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.21 @07:45:33.03 Hand.open @07:45:33.66 Forearm.reconfigure :loose @07:45:33.70 Forearm.seek :retract @07:45:34.61 collectSample 100ml w/bubble point of 24psi @07:45:34.62 Priming 7ml @07:45:34.63 Intake.select :close @07:45:34.64 Exhaust.select :close @07:45:34.65 SS.hold @07:45:34.85 Calibrating pressure and temperature sensors @07:45:34.88 CTV.dial :air @07:45:34.89 SSV.dial :spit,avoiding: :exhaust @07:45:34.91 SS.reconfigure :fast @07:45:34.95 SS.seek 5ml @07:45:37.89 SSV.dial CBV @07:45:37.91
CBV.dial SSV @07:45:39.24 delay 3.8 seconds @07:45:43.16 SSV.dial :spit @07:45:44.47 SS.seek 0ml @07:45:47.18 SSV.dial CBV @07:45:48.50 CTV.dial :intake @07:45:49.28 SSV.dial CBV @07:45:49.30
CBV.dial SSV @07:45:49.32 Allowing 40s for 7.0ml of remaining 7.0ml @07:45:49.33 Intake.select :open @07:45:51.48 Intake @15.0psia, vacuum from 9.5 to 12.5psi @07:45:51.49 SS.reconfigure :inhaling 2.20ml/s @07:45:51.53 SS.seek 7ml @07:45:58.82 Stroke completed -- waiting for pressure to equalize ... @07:45:58.83 SS.reconfigure :exhale @07:45:58.87 SS.hold @07:45:59.16 Intake.select :close @07:45:59.30 SSV.dial :exhaust @07:46:00.04 Exhaust.select :open @07:46:00.15 SS.seek 0ml @07:46:03.93 Exhaust.select :close @07:46:04.03 Sampling 100.0ml @07:46:04.04 Intake.select :close @07:46:04.05 Exhaust.select :close @07:46:04.06 SS.reconfigure :default @07:46:04.10 SS.hold @07:46:04.28 CTV.dial :intake @07:46:04.29 SSV.dial CBV @07:46:04.33
CBV.dial SSV @07:46:05.08 Allowing 130s for 25.0ml of remaining 100.0ml @07:46:05.09 Intake.select :open @07:46:07.24 Intake @15.0psia, vacuum from 9.5 to 12.5psi @07:46:07.25 SS.reconfigure :inhaling 2.20ml/s @07:46:07.29 SS.seek 25ml @07:46:22.93 Stroke completed -- waiting for pressure to equalize ... @07:46:22.94 SS.reconfigure :exhale @07:46:22.98 SS.hold @07:46:25.86 Intake.select :close @07:46:26.00 SSV.dial :exhaust @07:46:26.76 Exhaust.select :open @07:46:26.86 SS.seek 0ml @07:46:35.76 Exhaust.select :close @07:46:35.86 SSV.dial CBV @07:46:36.35
CBV.dial SSV @07:46:37.12 Allowing 130s for 25.0ml of remaining 75.0ml @07:46:37.13 Intake.select :open @07:46:39.28 Intake @15.2psia, vacuum from 9.7 to 12.7psi @07:46:39.29 SS.reconfigure :inhaling 2.20ml/s @07:46:39.34 SS.seek 25ml @07:46:54.29 Stroke completed -- waiting for pressure to equalize ... @07:46:54.30 SS.reconfigure :exhale @07:46:54.34 SS.hold @07:46:59.85 Intake.select :close @07:46:59.99 SSV.dial :exhaust @07:47:00.76 Exhaust.select :open @07:47:00.86 SS.seek 0ml @07:47:09.57 Exhaust.select :close @07:47:09.67 SSV.dial CBV @07:47:09.70
CBV.dial SSV @07:47:10.46 Allowing 130s for 25.0ml of remaining 50.0ml @07:47:10.47 Intake.select :open @07:47:12.62 Intake @15.2psia, vacuum from 9.7 to 12.7psi @07:47:12.64 SS.reconfigure :inhaling 2.20ml/s @07:47:12.69 SS.seek 25ml @07:47:28.08 Stroke completed -- waiting for pressure to equalize ... @07:47:28.09 SS.reconfigure :exhale @07:47:28.13 SS.hold @07:47:33.67 Intake.select :close @07:47:33.81 SSV.dial :exhaust @07:47:34.57 Exhaust.select :open @07:47:34.67 SS.seek 0ml @07:47:43.33 Exhaust.select :close @07:47:43.44 SSV.dial CBV @07:47:43.46
CBV.dial SSV @07:47:44.23 Allowing 130s for 25.0ml of remaining 25.0ml @07:47:44.24 Intake.select :open @07:47:46.39 Intake @15.0psia, vacuum from 9.5 to 12.5psi @07:47:46.40 SS.reconfigure :inhaling 2.20ml/s @07:47:46.44 SS.seek 25ml @07:48:02.07 Stroke completed -- waiting for pressure to equalize ... @07:48:02.08 SS.reconfigure :exhale @07:48:02.12 SS.hold @07:48:08.51 Intake.select :close @07:48:08.64 SSV.dial :exhaust @07:48:09.40 Exhaust.select :open @07:48:09.50 SS.seek 0ml @07:48:18.10 Exhaust.select :close @07:48:18.20 Sampled 100.0ml @07:48:18.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @07:48:18.22 Intake.select :close @07:48:18.28 CSV.dial :waste @07:48:18.34 CS.seek 0ml @07:48:18.40 SSV.dial CBV,avoiding: :exhaust @07:48:18.43
CBV.dial SSV @07:48:18.49 CSV.dial :air @07:48:19.22 CS.seek 10ml @07:48:20.30
CTV.dial :air @07:48:21.09 delay 15 seconds @07:48:36.11 SSV.dial :spit,avoiding: :exhaust @07:48:37.43 CTV.dial CSV,avoiding: :intake @07:48:37.47
SS.seek 0ml @07:48:37.58 SSV.dial CBV,avoiding: :exhaust @07:48:43.81 CBV.dial between SSV and waste2 @07:48:44.20 CTV.dial CSV,avoiding: :air @07:48:44.22
CSV.dial CTV @07:48:44.78 CS.reconfigure :pressurize @07:48:44.83 CS.seek 0ml @07:49:03.26 BubblePoint: Intake @39.2psia, Outlet @15.0psia @07:49:03.27 CBV.dial SSV @07:49:03.59 SS.reconfigure :default @07:49:03.63 SS.seek 10ml @07:49:09.38 delay 15 seconds @07:49:24.49 Evacuated: Intake @16.7psia, Outlet @9.5psia @07:49:24.50 SSV.dial :spit,avoiding: :exhaust @07:49:25.82 SS.reconfigure :exhale @07:49:25.86 SS.seek 0ml @07:49:31.11 SS.reconfigure :fast @07:49:31.15 SS.seek 5ml @07:49:34.10 SSV.dial CBV,avoiding: :exhaust @07:49:34.13
CBV.dial SSV @07:49:35.45 CTV.dial :air,avoiding: :intake @07:49:36.81 CSV.dial CTV @07:49:36.84
SS.seek 10ml @07:49:37.84 CSV.dial :air @07:49:38.28 CS.reconfigure :fast @07:49:38.32 CS.seek 0ml @07:49:39.30
SSV.dial :spit,avoiding: :exhaust @07:49:40.62 SS.reconfigure :exhale @07:49:40.66 SS.seek 0ml @07:49:45.69 SS.reconfigure :fast @07:49:45.73 SS.seek 5ml @07:49:45.98 CS.stop @07:49:48.85 SSV.dial CBV,avoiding: :exhaust @07:49:48.88
CBV.dial SSV @07:49:51.21 SSV.dial :spit,avoiding: :exhaust @07:49:52.51 SS.reconfigure :default @07:49:53.03 SS.seek 0ml @07:49:56.16 SS.stop @07:49:56.20 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @07:49:56.27 CSV.dial :waste @07:49:56.38 SSV.dial CBV,avoiding: :exhaust @07:49:56.40
CBV.dial SSV @07:49:57.03 CS.seek 0ml @07:49:57.10 CSV.dial :air @07:49:57.74
CTV.dial :air @07:49:57.76 delay 15 seconds @07:49:57.83 CS.seek 10ml @07:50:12.77
SSV.dial :spit,avoiding: :exhaust @07:50:14.09 CTV.dial CSV,avoiding: :intake @07:50:14.12
SS.reconfigure :exhale @07:50:14.21 SS.seek 0ml @07:50:14.66 SSV.dial CBV,avoiding: :exhaust @07:50:22.60 CBV.dial between SSV and waste2 @07:50:22.98 CTV.dial CSV,avoiding: :air @07:50:23.00
CSV.dial CTV @07:50:23.56 CS.reconfigure :pressurize @07:50:23.60 CS.seek 0ml @07:50:44.85 BubblePoint: Intake @38.2psia, Outlet @14.1psia @07:50:44.86 CBV.dial SSV @07:50:45.18 SS.reconfigure :default @07:50:45.23 SS.seek 10ml @07:50:50.94 delay 15 seconds @07:51:06.05 Evacuated: Intake @18.0psia, Outlet @11.7psia @07:51:06.06 SSV.dial :spit,avoiding: :exhaust @07:51:07.38 SS.reconfigure :exhale @07:51:07.43 SS.seek 0ml @07:51:12.64 SS.reconfigure :fast @07:51:12.68 SS.seek 5ml @07:51:15.62 SSV.dial CBV,avoiding: :exhaust @07:51:15.64
CBV.dial SSV @07:51:16.97 CTV.dial :air,avoiding: :intake @07:51:18.35 CSV.dial CTV @07:51:18.39
SS.seek 10ml @07:51:19.38 CSV.dial :air @07:51:19.81 CS.reconfigure :fast @07:51:19.85 CS.seek 0ml @07:51:20.82
SSV.dial :spit,avoiding: :exhaust @07:51:22.15 SS.reconfigure :exhale @07:51:22.19 SS.seek 0ml @07:51:25.07 CS.stop @07:51:27.31
SS.reconfigure :fast @07:51:27.35 SS.seek 5ml @07:51:30.38 SSV.dial CBV,avoiding: :exhaust @07:51:30.40
CBV.dial SSV @07:51:32.74 SSV.dial :spit,avoiding: :exhaust @07:51:34.08 SS.reconfigure :default @07:51:34.13 SS.seek 0ml @07:51:37.42 SS.stop @07:51:37.46 Lysing: Adding 2.0ml AqMeOH @07:51:37.47 CBV.dial between CSV and waste1,avoiding: :DBV @07:51:38.95 CTV.dial CSV @07:51:40.29 CSV.dial CSR @07:51:41.07 Pulling 1ml of CSR.AqMeOH @07:51:41.08 CSR.select :AqMeOH @07:51:41.19 CS.reconfigure :default @07:51:41.23 CS.seek 1ml @07:51:44.73 delay 5 seconds @07:51:49.74 CSR.select :air @07:51:49.89 CS.seek 6ml @07:52:03.51 delay 2 seconds @07:52:05.52 CSV.dial :waste2 @07:52:06.07 CS.reconfigure :fast @07:52:06.12 CS.seek 0ml @07:52:20.57 CSV.dial CSR @07:52:21.04 Pulling 2ml of CSR.AqMeOH @07:52:21.05 CSR.select :AqMeOH @07:52:21.16 CS.reconfigure :default @07:52:21.20 CS.seek 2ml @07:52:27.10 delay 5 seconds @07:52:32.12 CSR.select :air @07:52:32.27 CS.seek 5ml @07:52:41.01 delay 2 seconds @07:52:43.02 CSV.dial CTV @07:52:43.46 CS.seek 1ml @07:52:54.70 Before lysing Collection Puck Heater at 22C/37.5psia @07:52:54.72 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @07:52:54.73 CH.seek 70C,8:00 @07:54:57.88 CH.hold 10:00,70C @07:54:58.06 Begun lysing Collection Puck Heater at 68.1C/44.9psia @08:04:58.06 Ended lysing Collection Puck Heater at 70C/42.7psia @08:04:58.08 CS.seek 0ml @08:05:01.75 CTV.dial between spare and air,avoiding: :intake @08:05:02.79 CSV.dial :air @08:05:03.24 CS.seek 5ml @08:05:17.02 CSV.dial CTV @08:05:17.46 delay 2 seconds @08:05:19.48 CSV.dial :air @08:05:20.04 delay 2 seconds @08:05:22.05 CSV.dial CTV @08:05:22.49 delay 2 seconds @08:05:24.51 CSV.dial :air @08:05:24.95 delay 2 seconds @08:05:26.97 CS.seek 0ml @08:05:40.67 Puck @67.9psia after final lysis pressurization @08:05:40.68 Recovering Lysate into Collection syringe @08:05:40.69 CSV.dial CBV @08:05:41.70 CBV.dial CSV @08:05:42.05 CS.seek 5ml @08:05:55.80 delay 5 seconds @08:06:00.82 CTV.dial :air @08:06:01.35 CS.seek 7ml @08:06:07.30 delay 5 seconds @08:06:12.32 CSV.dial :air2 @08:06:12.78 CS.seek 10ml @08:06:21.31 CSV.dial between air and CTV,avoiding: CBV @08:06:22.47 Lysing Complete @08:06:22.53 Storing sh1 puck in Collection Clamp to tube 1 @08:06:22.69 SC.reconfigure :tight @08:06:22.73 SC.seek 1 @08:06:26.33 SC.configure :limp @08:06:26.47 SE.reconfigure :place @08:06:26.51 SE.seek :beamBreak @08:06:26.57
Elbow.reconfigure :tight @08:06:26.61 Elbow.seek CC @08:06:27.32 Elbow.reconfigure :loose @08:06:27.41 Forearm.seek CC @08:06:28.39 Hand.close @08:06:29.08 CC.seek :open @08:06:29.33 SE.configure :default @08:06:29.38 SE.seek :place @08:06:33.80
Forearm.seek :retract @08:06:34.78 Elbow.reconfigure :tight @08:06:34.83 Elbow.seek 1 @08:06:36.51 Elbow.reconfigure :limp @08:06:36.56 Forearm.seek 1 @08:06:37.58 Elbow.hold @08:06:37.64 Elbow.reconfigure :tight @08:06:37.68 Hand.open @08:06:38.44 Forearm.force -20 @08:06:38.47 Forearm.seek 1 + 320 counts @08:06:38.99 Forearm.reconfigure :tight @08:06:39.03 Forearm.seek 1 + 450 counts @08:06:39.28 SE.seek :down @08:06:40.41
Forearm.reconfigure :loose @08:06:40.45 Forearm.seek :retract @08:06:41.97 SE.stop @08:06:42.01
Tranferring DA @08:06:42.02 Beginning samplePassC @08:06:42.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:06:42.21 Elbow.seek FlushPuck::Garage @08:06:44.01 Elbow.reconfigure :loose @08:06:44.10 Forearm.seek :garage @08:06:45.03 Hand.close @08:06:45.69 Forearm.seek :retract @08:06:46.61 Elbow.reconfigure :tight @08:06:46.65 Elbow.seek CC @08:06:49.59 Elbow.reconfigure :loose @08:06:49.68 Forearm.reconfigure :tight @08:06:49.72 Forearm.seek CC @08:06:50.90 CC.seek :closed @08:06:55.42 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.35 @08:06:55.49 Hand.open @08:06:56.14 Forearm.reconfigure :loose @08:06:56.18 Forearm.seek :retract @08:06:57.17 PSV.dial PSR @08:06:58.56 Pulling 1.5ml of PSR.flush PSR.select :flush @08:06:58.68 PS.seek 1.5ml @08:07:14.79 delay 5 seconds @08:07:19.81 PSV.dial :waste1 @08:07:20.29 PS.seek 0ml @08:07:36.38 PSV.dial PSR @08:07:36.83 PSR.select :air @08:07:36.94 PS.seek 2.5ml @08:08:03.29 PRV.dial CBV,avoiding: :ab2 @08:08:03.31
CBV.dial PRV @08:08:04.10 PSV.dial :mixloop @08:08:05.14 CTV.dial :air,avoiding: :intake @08:08:05.16 PTV.dial :PRVmixing @08:08:05.91 PS.seek 0ml @08:08:32.25 PSV.dial :air @08:08:32.71 PS.seek 2.5ml @08:08:59.03 PSV.dial :mixloop @08:08:59.51 PS.seek 0ml @08:09:25.87 PSV.dial :air @08:09:26.48 PS.seek 2.5ml @08:09:52.87 PSV.dial :mixloop @08:09:53.32 PS.seek 0ml @08:10:19.84 Garaging FlushPuck in Collection Clamp @08:10:19.91 Elbow.reconfigure :tight @08:10:19.95 Elbow.seek CC @08:10:20.54 Elbow.reconfigure :loose @08:10:20.62 Forearm.seek CC @08:10:21.64 Hand.close @08:10:22.20 CC.seek :open @08:10:26.82 Forearm.seek :retract @08:10:27.78 Elbow.reconfigure :tight @08:10:27.82 Elbow.seek :garage @08:10:30.72 Elbow.reconfigure :loose @08:10:30.81 Forearm.reconfigure :tight @08:10:30.85 Forearm.seek :garage @08:10:31.89 Hand.open @08:10:32.51 Forearm.reconfigure :loose @08:10:32.55 Forearm.seek :retract @08:10:33.53 PSV.dial :mixloop @08:10:33.54 PTV.dial :PRVmixing @08:10:33.55 PRV.dial CBV @08:10:33.57
CBV.dial PRV @08:10:33.59 CTV.dial :air,avoiding: :intake @08:10:33.61 Loading lyfil puck from tube 2 into Collection Clamp @08:10:33.82 SC.reconfigure :tight @08:10:33.86 SC.seek 2 @08:10:37.54 SC.configure :limp @08:10:37.74 Elbow.reconfigure :tight @08:10:37.78 Elbow.seek 2 @08:10:39.67 Forearm.reconfigure :tight @08:10:39.71 Forearm.seek 2 + 450 counts @08:10:40.88 SE.reconfigure :pick @08:10:40.93 SE.seek :beamBreak @08:10:44.00 SE.configure :default @08:10:44.14 Elbow.seek 2 @08:10:44.30 Forearm.seek 2 + 300 counts @08:10:44.51 Forearm.reconfigure :loose @08:10:44.55 Hand.close @08:10:45.21 SE.seek :down @08:10:45.40
Forearm.seek :retract @08:10:46.34 Elbow.seek CC @08:10:47.97 Elbow.reconfigure :loose @08:10:48.03 SE.stop @08:10:48.14
Forearm.reconfigure :tight @08:10:48.18 Forearm.seek CC @08:10:49.47 CC.seek :closed @08:10:54.51 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @08:10:54.52 Hand.open @08:10:55.13 Forearm.reconfigure :loose @08:10:55.18 Forearm.seek :retract @08:10:56.14 CTV.dial CSV,avoiding: :intake @08:10:57.50 CSV.dial CTV @08:10:57.82 PS.seek 2.5ml @08:11:24.16 CS.seek 4.501ml @08:11:39.08 delay 8 seconds @08:11:47.10 PSV.dial :air @08:11:47.56 delay 2 seconds @08:11:49.58 PSV.dial :waste2 @08:11:50.33 PS.seek 0ml @08:12:16.71 CBV.dial between PRV and waste1 @08:12:17.03 CSV.dial :air @08:12:17.51 CS.seek 10ml @08:12:32.49 CSV.dial CTV @08:12:32.94 CS.seek 0ml @08:12:59.33 CBV.dial :waste1 @08:12:59.63 delay 5 seconds @08:13:04.64 CTV.dial :air,avoiding: :intake @08:13:06.06 Storing lyfil puck in Collection Clamp to tube 1 @08:13:06.21 SC.reconfigure :tight @08:13:06.26 SC.seek 1 @08:13:09.88 SC.configure :limp @08:13:10.07 Elbow.reconfigure :tight @08:13:10.12 Elbow.seek 1 @08:13:11.86 Forearm.reconfigure :tight @08:13:11.90 Forearm.seek 1 + 450 counts @08:13:12.91 SE.reconfigure :pick @08:13:12.96 SE.seek :beamBreak @08:13:16.04 SE.configure :default @08:13:16.18 Elbow.reconfigure :limp @08:13:16.22 Forearm.reconfigure :loose @08:13:16.26 Forearm.seek 1 @08:13:16.56 Elbow.hold @08:13:16.61 Elbow.reconfigure :tight @08:13:16.70 SE.seek 0.15 Pucks below Top Plate @08:13:17.14 Forearm.force -20 @08:13:17.18 Forearm.seek 1 + 313 counts @08:13:17.59 Forearm.reconfigure :tight @08:13:17.64 Forearm.seek 1 + 450 counts @08:13:17.92 SE.seek 1.15 Pucks below Top Plate @08:13:18.45 Forearm.reconfigure :loose @08:13:18.49 Forearm.seek :retract @08:13:19.42 Elbow.seek CC @08:13:21.13 Elbow.reconfigure :loose @08:13:21.27 Forearm.seek CC @08:13:22.23 Hand.close @08:13:22.83 CC.seek :open @08:13:27.57 Forearm.seek :retract @08:13:28.53 Elbow.reconfigure :tight @08:13:28.57 Elbow.seek 1 @08:13:30.33 Elbow.reconfigure :limp @08:13:30.38 Forearm.seek 1 @08:13:31.35 Elbow.hold @08:13:31.41 Elbow.reconfigure :tight @08:13:31.46 Hand.open @08:13:32.18 Forearm.force -20 @08:13:32.21 Forearm.seek 1 + 317 counts @08:13:32.57 Forearm.reconfigure :tight @08:13:32.62 Forearm.seek 1 + 450 counts @08:13:32.95 SE.seek :down @08:13:34.07
Forearm.reconfigure :loose @08:13:34.11 Forearm.seek :retract @08:13:35.61 SE.stop @08:13:35.65
PSV.dial :air @08:13:36.53 PS.seek 2.5ml @08:14:02.96 delay 1 second @08:14:03.98 PSV.dial :mixloop @08:14:04.45 delay 1 second @08:14:05.47 PSV.dial :air @08:14:05.93 delay 1 second @08:14:06.95 PSV.dial :mixloop @08:14:07.40 delay 1 second @08:14:08.41 PSV.dial :air @08:14:08.89 delay 1 second @08:14:10.37 PS.seek 0ml @08:14:36.75 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @08:14:36.91 Elbow.seek FlushPuck::Garage @08:14:38.73 Elbow.reconfigure :loose @08:14:38.82 Forearm.seek :garage @08:14:39.82 Hand.close @08:14:40.56 Forearm.seek :retract @08:14:41.57 Elbow.reconfigure :tight @08:14:41.62 Elbow.seek PC @08:14:42.88 Elbow.reconfigure :loose @08:14:42.96 Forearm.reconfigure :tight @08:14:43.01 Forearm.seek PC @08:14:44.18 PC.seek :closed @08:14:48.82 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.65 @08:14:48.89 Hand.open @08:14:49.50 Forearm.reconfigure :loose @08:14:49.55 Forearm.seek :retract @08:14:50.48 PRV.dial :air @08:14:50.93 PSV.dial :mixloop @08:14:51.45 PS.seek 0.3ml @08:14:55.30 PSV.dial PSR @08:14:56.41 Pulling 0.4ml of PSR.air PSR.select :air @08:14:56.44 PS.seek 0.7ml @08:15:01.34 delay 3 seconds @08:15:04.36 PSR.select :air @08:15:04.38 PS.seek 2.5ml @08:15:23.56 delay 3 seconds @08:15:26.57 PSV.dial :mixloop @08:15:27.62 PTV.dial :mixing @08:15:28.37 PBV.dial :waste2 @08:15:29.31 PS.seek 0ml @08:15:55.71 Garaging FlushPuck in Processing Clamp @08:15:55.78 Elbow.reconfigure :tight @08:15:55.82 Elbow.seek PC @08:15:55.92 Elbow.reconfigure :loose @08:15:56.01 Forearm.seek PC @08:15:56.99 Hand.close @08:15:57.57 PC.seek :open @08:16:02.32 Forearm.seek :retract @08:16:03.31 Elbow.reconfigure :tight @08:16:03.35 Elbow.seek :garage @08:16:04.69 Elbow.reconfigure :loose @08:16:04.77 Forearm.reconfigure :tight @08:16:04.82 Forearm.seek :garage @08:16:05.87 Hand.open @08:16:06.50 Forearm.reconfigure :loose @08:16:06.54 Forearm.seek :retract @08:16:07.50 Loading da puck from tube 2 into Processing Clamp @08:16:07.72 SC.reconfigure :tight @08:16:07.76 SC.seek 2 @08:16:11.34 SC.configure :limp @08:16:11.53 Elbow.reconfigure :tight @08:16:11.57 Elbow.seek 2 @08:16:13.43 Forearm.reconfigure :tight @08:16:13.47 Forearm.seek 2 + 450 counts @08:16:14.52 SE.reconfigure :pick @08:16:14.57 SE.seek :beamBreak @08:16:17.61 SE.configure :default @08:16:17.75 Elbow.seek 2 @08:16:17.92 Forearm.seek 2 + 300 counts @08:16:18.13 Forearm.reconfigure :loose @08:16:18.17 Hand.close @08:16:18.83 Puck::Warning in MAIN -- Loaded Last remaining Puck (from Tube 2) @08:16:18.85 SE.seek :down @08:16:19.03
Forearm.seek :retract @08:16:19.95 Elbow.seek PC @08:16:21.63 SE.stop @08:16:22.47
Elbow.reconfigure :loose @08:16:22.55 Forearm.reconfigure :tight @08:16:22.60 Forearm.seek PC @08:16:24.23 PC.seek :closed @08:16:28.88 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.68 @08:16:28.89 Hand.open @08:16:29.51 Forearm.reconfigure :loose @08:16:29.55 Forearm.seek :retract @08:16:30.50 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @08:16:30.52 PH.seek 28C,5:00 @08:17:16.60 PH.hold 2:00:00,28C @08:17:16.65 delay 1:30 @08:18:46.69 PSR.select :air @08:18:46.70 PSV.dial PSR @08:18:47.81 Pulling 0.5ml of PSR.wash @08:18:47.82 PSR.select :wash @08:18:47.93 PS.seek 0.5ml @08:18:53.93 delay 5 seconds @08:18:58.95 PSR.select :air @08:18:59.06 PS.seek 2ml @08:19:15.21 PTV.dial :puck @08:19:15.93 PSV.dial PTV @08:19:16.69 PBV.dial :waste1 @08:19:18.07 PS.seek 1.5ml @08:19:24.11 delay 3 seconds @08:19:27.13 PBV.dial between PSV and waste1 @08:19:27.50 PS.seek 0ml @08:19:43.55 delay 5:00 @08:24:43.57 recoverPSRreagent08 @08:24:43.58 PTV.dial :puck @08:24:43.59 PBV.dial :waste1 @08:24:43.95 delay 30 seconds @08:25:13.97 PBV.dial between PSV and waste1 @08:25:14.33 PSR.select :air @08:25:14.34 PSV.dial PSR @08:25:15.11 PS.reconfigure :fast @08:25:15.16 PS.seek 2.5ml @08:25:39.48 PSV.dial PTV @08:25:40.23 delay 5 seconds @08:25:45.25 PSV.dial PSR @08:25:46.01 delay 5 seconds @08:25:51.03 PSV.dial PTV @08:25:51.79 delay 5 seconds @08:25:56.81 PSV.dial :air @08:25:57.58 PS.seek 0ml @08:26:21.29 PTV.dial :PRVmixing,via: PRV @08:26:22.62 PSV.dial :mixloop @08:26:23.09 PRV.dial :ab1 @08:26:23.83 PS.reconfigure :slow @08:26:23.87 PS.seek 0.15ml @08:26:27.42 delay 2 seconds @08:26:29.43 CBV.dial PRV @08:26:29.46
PRV.dial CBV @08:26:30.03 PS.seek 0.36ml @08:26:34.86 delay 2 seconds @08:26:36.87 CBV.dial between PRV and PSV @08:26:37.18 PRV.dial :air @08:26:37.63 PS.seek 0.9ml @08:26:49.20 PRV.dial :waste2 @08:26:49.94 PS.seek 0.5ml @08:26:58.64 addPMLReagent08 @08:26:58.65 PSV.dial :air @08:26:59.11 PS.reconfigure :default @08:26:59.15 PS.seek 2ml @08:27:15.45 PSV.dial :PML @08:27:15.90 PTV.dial :mixing @08:27:16.64 PBV.dial :waste1 @08:27:17.04 PS.seek 1.85ml @08:27:19.38 PBV.dial between PSV and waste1 @08:27:19.75 PS.seek 0ml @08:27:39.70 delay 2 seconds @08:27:41.71 Beginning samplePassC @08:27:41.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @08:27:41.88 Elbow.reconfigure :tight @08:27:41.93 Elbow.seek FlushPuck::Garage @08:27:43.27 Elbow.reconfigure :loose @08:27:43.36 Forearm.seek :garage @08:27:44.76 Hand.close @08:27:45.55 Forearm.seek :retract @08:27:46.50 Elbow.reconfigure :tight @08:27:46.54 Elbow.seek CC @08:27:49.35 Elbow.reconfigure :loose @08:27:49.44 Forearm.reconfigure :tight @08:27:49.49 Forearm.seek CC @08:27:50.75 CC.seek :closed @08:27:55.27 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.40 @08:27:55.35 Hand.open @08:27:56.00 Forearm.reconfigure :loose @08:27:56.04 Forearm.seek :retract @08:27:57.03 PSV.dial PSR @08:27:58.14 Pulling 1.5ml of PSR.flush @08:27:58.15 PSR.select :flush @08:27:58.27 PS.seek 1.5ml @08:28:14.48 delay 5 seconds @08:28:19.50 PSV.dial :waste1 @08:28:19.95 PS.seek 0ml @08:28:36.29 PSV.dial PSR @08:28:36.74 PSR.select :air @08:28:36.85 PS.seek 2.5ml @08:29:03.23 PRV.dial CBV,avoiding: :ab2 @08:29:03.25
CBV.dial PRV @08:29:04.91 PSV.dial :mixloop @08:29:05.95 CTV.dial :air,avoiding: :intake @08:29:05.96 PTV.dial :PRVmixing @08:29:06.72 PS.seek 0ml @08:29:33.01 PSV.dial :air @08:29:33.47 PS.seek 2.5ml @08:29:59.82 PSV.dial :mixloop @08:30:00.28 PS.seek 0ml @08:30:26.63 PSV.dial :air @08:30:27.09 PS.seek 2.5ml @08:30:53.41 PSV.dial :mixloop @08:30:53.89 PS.seek 0ml @08:31:20.16 Garaging FlushPuck in Collection Clamp @08:31:20.23 Elbow.reconfigure :tight @08:31:20.28 Elbow.seek CC @08:31:20.38 Elbow.reconfigure :loose @08:31:20.47 Forearm.seek CC @08:31:21.42 Hand.close @08:31:22.19 CC.seek :open @08:31:26.83 Forearm.seek :retract @08:31:27.79 Elbow.reconfigure :tight @08:31:27.83 Elbow.seek :garage @08:31:30.83 Elbow.reconfigure :loose @08:31:30.92 Forearm.reconfigure :tight @08:31:30.96 Forearm.seek :garage @08:31:31.89 Hand.open @08:31:32.62 Forearm.reconfigure :loose @08:31:32.66 Forearm.seek :retract @08:31:33.61 delayUntil "08:35:41.70" @08:35:41.72 recoverPRVreagent08 PTV.dial :mixing @08:35:42.49 PBV.dial :waste1 @08:35:42.85 delay 30 seconds @08:36:12.87 PBV.dial between PSV and waste1 @08:36:13.24 PSV.dial :air @08:36:13.71 PS.reconfigure :fast @08:36:13.75 PS.seek 2.5ml @08:36:38.09 PSV.dial :mixloop @08:36:38.55 delay 2 seconds @08:36:40.56 PSV.dial :air @08:36:41.02 delay 2 seconds @08:36:43.03 PSV.dial :mixloop @08:36:43.49 delay 2 seconds @08:36:45.50 PSV.dial :air @08:36:45.98 PS.seek 0ml @08:37:09.56 washCycleDA #1 of 2 @08:37:09.57 PSR.select :air @08:37:09.59 PSV.dial PSR @08:37:10.96 Pulling 0.5ml of PSR.wash @08:37:10.97 PSR.select :wash @08:37:11.08 PS.reconfigure :default @08:37:11.12 PS.seek 0.5ml @08:37:17.03 delay 5 seconds @08:37:22.05 PSR.select :air @08:37:22.16 PS.seek 2ml @08:37:38.25 PTV.dial :puck @08:37:38.98 PSV.dial PTV @08:37:39.73 PBV.dial :waste1 @08:37:40.15 PS.seek 1.5ml @08:37:46.06 delay 3 seconds @08:37:49.08 PBV.dial between PSV and waste1 @08:37:49.92 PS.seek 0ml @08:38:06.19 delay 2:00 @08:40:06.21 recoverPSRreagent08 @08:40:06.22 PTV.dial :puck @08:40:06.23 PBV.dial :waste1 @08:40:06.59 delay 30 seconds @08:40:36.62 PBV.dial between PSV and waste1 @08:40:36.98 PSR.select :air @08:40:37.00 PSV.dial PSR @08:40:37.77 PS.reconfigure :fast @08:40:37.81 PS.seek 2.5ml @08:41:02.12 PSV.dial PTV @08:41:03.01 delay 5 seconds @08:41:08.03 PSV.dial PSR @08:41:08.78 delay 5 seconds @08:41:13.80 PSV.dial PTV @08:41:14.56 delay 5 seconds @08:41:19.58 PSV.dial :air @08:41:20.47 PS.seek 0ml @08:41:44.15 washCycleDA #2 of 2 @08:41:44.16 PSR.select :air @08:41:44.18 PSV.dial PSR @08:41:45.55 Pulling 0.5ml of PSR.wash @08:41:45.56 PSR.select :wash @08:41:45.67 PS.reconfigure :default @08:41:45.71 PS.seek 0.5ml @08:41:51.62 delay 5 seconds @08:41:56.64 PSR.select :air @08:41:56.75 PS.seek 2ml @08:42:12.87 PTV.dial :puck @08:42:12.88 PSV.dial PTV @08:42:13.63 PBV.dial :waste1 @08:42:14.07 PS.seek 1.5ml @08:42:20.06 delay 3 seconds @08:42:23.07 PBV.dial between PSV and waste1 @08:42:23.45 PS.seek 0ml @08:42:39.53 delay 2:00 @08:44:39.55 recoverPSRreagent08 PTV.dial :puck @08:44:39.56 PBV.dial :waste1 @08:44:39.93 delay 30 seconds @08:45:09.95 PBV.dial between PSV and waste1 @08:45:10.32 PSR.select :air @08:45:10.34 PSV.dial PSR @08:45:11.10 PS.reconfigure :fast @08:45:11.15 PS.seek 2.5ml @08:45:35.36 PSV.dial PTV @08:45:36.13 delay 5 seconds @08:45:41.15 PSV.dial PSR @08:45:41.91 delay 5 seconds @08:45:46.93 PSV.dial PTV @08:45:47.69 delay 5 seconds @08:45:52.71 PSV.dial :air @08:45:53.46 PS.seek 0ml @08:46:17.24 getPMLReagent08 @08:46:17.25 PRV.dial :air @08:46:17.69 delay 5 seconds @08:46:22.71 PTV.dial :PRVmixing @08:46:24.04 delay 5 seconds @08:46:29.05 PRV.dial :ab2 @08:46:29.52 PTV.dial :PRVmixing @08:46:29.54 PSV.dial :mixloop @08:46:29.99 PS.reconfigure :default @08:46:30.04 PS.seek 0.3ml @08:46:34.02 delay 2 seconds @08:46:36.04 PRV.dial :air @08:46:36.55 PS.seek 0.55ml @08:46:40.12 addPMLReagent08 @08:46:40.13 PSV.dial :air @08:46:40.59 PS.seek 2ml @08:46:56.17 PSV.dial :PML @08:46:56.63 PTV.dial :mixing @08:46:57.38 PBV.dial :waste1 @08:46:57.80 PS.seek 1.85ml @08:47:00.14 PBV.dial between PSV and waste1 @08:47:00.53 PS.seek 0ml @08:47:20.21 delay 2 seconds @08:47:22.22 delay 8:00 @08:55:22.24 recoverPRVreagent08 @08:55:22.25 PTV.dial :mixing @08:55:22.26 PBV.dial :waste1 @08:55:22.62 delay 30 seconds @08:55:52.64 PBV.dial between PSV and waste1 @08:55:53.01 PSV.dial :air @08:55:53.48 PS.reconfigure :fast @08:55:53.53 PS.seek 2.5ml @08:56:17.88 PSV.dial :mixloop @08:56:18.34 delay 2 seconds @08:56:20.35 PSV.dial :air @08:56:20.81 delay 2 seconds @08:56:22.82 PSV.dial :mixloop @08:56:23.27 delay 2 seconds @08:56:25.29 PSV.dial :air @08:56:25.75 PS.seek 0ml @08:56:49.42 washCycleDA #1 of 3 @08:56:49.43 PSR.select :air @08:56:49.44 PSV.dial PSR @08:56:50.81 Pulling 0.5ml of PSR.wash @08:56:50.82 PSR.select :wash @08:56:50.93 PS.reconfigure :default @08:56:50.97 PS.seek 0.5ml @08:56:56.95 delay 5 seconds @08:57:01.96 PSR.select :air @08:57:02.08 PS.seek 2ml @08:57:18.16 PTV.dial :puck @08:57:18.90 PSV.dial PTV @08:57:19.65 PBV.dial :waste1 @08:57:20.06 PS.seek 1.5ml @08:57:26.04 delay 3 seconds @08:57:29.06 PBV.dial between PSV and waste1 @08:57:29.44 PS.seek 0ml @08:57:45.54 delay 2:00 @08:59:45.57 recoverPSRreagent08 @08:59:45.58 PTV.dial :puck @08:59:45.59 PBV.dial :waste1 @08:59:45.96 delay 30 seconds @09:00:15.98 PBV.dial between PSV and waste1 @09:00:16.33 PSR.select :air @09:00:16.35 PSV.dial PSR @09:00:17.12 PS.reconfigure :fast @09:00:17.16 PS.seek 2.5ml @09:00:41.47 PSV.dial PTV @09:00:42.22 delay 5 seconds @09:00:47.24 PSV.dial PSR @09:00:48.54 delay 5 seconds @09:00:53.56 PSV.dial PTV @09:00:54.77 delay 5 seconds @09:00:59.79 PSV.dial :air @09:01:00.56 PS.seek 0ml @09:01:24.22 washCycleDA #2 of 3 @09:01:24.23 PSR.select :air @09:01:24.25 PSV.dial PSR @09:01:25.62 Pulling 0.5ml of PSR.wash @09:01:25.63 PSR.select :wash @09:01:25.74 PS.reconfigure :default @09:01:25.79 PS.seek 0.5ml @09:01:31.76 delay 5 seconds @09:01:36.77 PSR.select :air @09:01:36.89 PS.seek 2ml @09:01:52.97 PTV.dial :puck @09:01:52.98 PSV.dial PTV @09:01:53.83 PBV.dial :waste1 @09:01:54.23 PS.seek 1.5ml @09:02:00.25 delay 3 seconds @09:02:03.27 PBV.dial between PSV and waste1 @09:02:03.63 PS.seek 0ml @09:02:19.82 delay 2:00 @09:04:19.84 recoverPSRreagent08 @09:04:19.85 PTV.dial :puck @09:04:19.86 PBV.dial :waste1 @09:04:20.22 delay 30 seconds @09:04:50.24 PBV.dial between PSV and waste1 @09:04:50.59 PSR.select :air @09:04:50.61 PSV.dial PSR @09:04:51.36 PS.reconfigure :fast @09:04:51.41 PS.seek 2.5ml @09:05:15.47 PSV.dial PTV @09:05:16.23 delay 5 seconds @09:05:21.25 PSV.dial PSR @09:05:22.01 delay 5 seconds @09:05:27.03 PSV.dial PTV @09:05:27.80 delay 5 seconds @09:05:32.82 PSV.dial :air @09:05:33.58 PS.seek 0ml @09:05:57.47 washCycleDA #3 of 3 @09:05:57.48 PSR.select :air @09:05:57.49 PSV.dial PSR @09:05:58.86 Pulling 0.5ml of PSR.wash @09:05:58.87 PSR.select :wash @09:05:58.99 PS.reconfigure :default @09:05:59.03 PS.seek 0.5ml @09:06:05.03 delay 5 seconds @09:06:10.05 PSR.select :air @09:06:10.16 PS.seek 2ml @09:06:26.34 PTV.dial :puck @09:06:26.35 PSV.dial PTV @09:06:27.09 PBV.dial :waste1 @09:06:27.49 PS.seek 1.5ml @09:06:33.43 delay 3 seconds @09:06:36.45 PBV.dial between PSV and waste1 @09:06:36.82 PS.seek 0ml @09:06:52.94 delay 2:00 @09:08:52.96 recoverPSRreagent08 @09:08:52.97 PTV.dial :puck @09:08:52.98 PBV.dial :waste1 @09:08:53.34 delay 30 seconds @09:09:23.36 PBV.dial between PSV and waste1 @09:09:23.71 PSR.select :air @09:09:23.73 PSV.dial PSR @09:09:24.50 PS.reconfigure :fast @09:09:24.55 PS.seek 2.5ml @09:09:48.90 PSV.dial PTV @09:09:49.65 delay 5 seconds @09:09:54.67 PSV.dial PSR @09:09:55.43 delay 5 seconds @09:10:00.45 PSV.dial PTV @09:10:01.21 delay 5 seconds @09:10:06.23 PSV.dial :air @09:10:07.11 PS.seek 0ml @09:10:30.77 Gate.power :camera,:ON @09:10:30.79 Mixing DA Substrates @09:10:30.80 PSR.select :air @09:10:30.81 PSV.dial PSR @09:10:32.39 Pulling 0.25ml of PSR.sub1 @09:10:32.43 PSR.select :sub1 @09:10:32.64 PS.reconfigure :default @09:10:32.76 PS.seek 0.25ml @09:10:36.16 delay 5 seconds @09:10:41.23 Pulling 0.25ml of PSR.sub2 PSR.select :sub2 @09:10:41.34 PS.seek 0.5ml @09:10:44.81 delay 5 seconds @09:10:49.83 PSR.select :air @09:10:49.93 PS.seek 2ml @09:11:06.12 PTV.dial :puck @09:11:06.13 PSV.dial PTV @09:11:06.91 PBV.dial :waste1 @09:11:07.35 PS.seek 1.5ml @09:11:13.37 delay 3 seconds @09:11:16.38 PBV.dial between PSV and waste1 @09:11:16.78 PS.seek 0ml @09:11:32.93 delay 10 seconds @09:11:42.96 recoverPSRreagent08 PTV.dial :puck @09:11:42.97 PBV.dial :waste1 @09:11:43.34 delay 30 seconds @09:12:13.36 PBV.dial between PSV and waste1 @09:12:13.74 PSR.select :air @09:12:13.76 PSV.dial PSR @09:12:14.56 PS.reconfigure :fast @09:12:14.61 PS.seek 2.5ml @09:12:39.74 PSV.dial PTV @09:12:40.65 delay 5 seconds @09:12:45.67 PSV.dial PSR @09:12:46.43 delay 5 seconds @09:12:51.45 PSV.dial PTV @09:12:52.22 delay 5 seconds @09:12:57.24 PSV.dial :air @09:12:58.04 PS.seek 0ml @09:13:22.96 Additional air clear @09:13:22.97 PSR.select :air @09:13:22.98 PSV.dial PSR @09:13:24.43 Pulling 2ml of PSR.air @09:13:24.44 PSR.select :air @09:13:24.46 PS.reconfigure :default @09:13:24.50 PS.seek 2ml @09:13:45.93 delay 5 seconds @09:13:50.94 PTV.dial :puck @09:13:50.96 PSV.dial PTV @09:13:51.74 PBV.dial :waste1 @09:13:52.17 PS.seek 1.5ml @09:13:58.11 delay 3 seconds @09:14:01.13 PBV.dial between PSV and waste1 @09:14:01.52 delay 1 second @09:14:02.96 PSV.dial :waste1 @09:14:04.10 PS.seek 0ml @09:14:20.29 Processing Puck Heater at 28.2C before imaging @09:14:20.30 PH.coast @09:14:20.42 Imaging da puck in Processing Clamp while storing it to tube 1 @09:14:20.58 SC.reconfigure :tight @09:14:20.62 SC.seek 1 @09:14:24.30 SC.configure :limp @09:14:24.49 Elbow.reconfigure :tight @09:14:24.53 Elbow.seek 1 @09:14:26.50 Forearm.reconfigure :tight @09:14:26.54 Forearm.seek 1 + 450 counts @09:14:27.61 SE.reconfigure :pick @09:14:27.67 SE.seek :beamBreak @09:14:30.71 SE.configure :default @09:14:30.85 Elbow.reconfigure :limp @09:14:30.89 Forearm.reconfigure :loose @09:14:30.93 Forearm.seek 1 @09:14:31.23 Elbow.hold @09:14:31.29 Elbow.reconfigure :tight @09:14:31.38 SE.seek 1.15 Pucks below Top Plate @09:14:31.95 Forearm.force -20 @09:14:31.99 Forearm.seek 1 + 307 counts @09:14:32.28 Forearm.reconfigure :tight @09:14:32.33 Forearm.seek 1 + 450 counts @09:14:32.56 SE.seek 2.15 Pucks below Top Plate @09:14:33.12 Forearm.reconfigure :loose @09:14:33.16 Forearm.seek :retract @09:14:34.08 Elbow.seek PC @09:14:36.77 Elbow.reconfigure :loose @09:14:36.85 Forearm.seek PC @09:14:37.82 Hand.close @09:14:38.53 PC.seek :open @09:14:43.27 Forearm.seek :retract @09:14:44.28 Elbow.reconfigure :tight @09:14:44.32 Elbow.seek 1 @09:14:46.96 Forearm.reconfigure :tight @09:14:47.01 Forearm.seek 1 + 450 counts @09:14:48.01 Hand.open @09:14:48.71 SE.seek 1.8 Pucks below Top Plate @09:14:49.00 SE.reconfigure :seal @09:14:49.05 SE.seek 0.84 Pucks below Top Plate @09:14:49.60 Lights, camera, ACTION! @09:14:49.63 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21jul2709h100ml.tif @09:15:09.10 Waiting >20 seconds for da to relinquish FlushPuck @09:15:41.41 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 48.474 seconds /var/log/esp/da21jul2709h100ml.tif: TIFF Upload Complete @09:15:41.44 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21jul2709h100ml15s.tif @09:15:57.12 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21jul2709h100ml15s.tif: TIFF Upload Complete @09:15:57.14 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21jul2709h100ml30s.tif @09:16:27.88 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21jul2709h100ml30s.tif: TIFF Upload Complete @09:16:27.90 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21jul2709h100ml70s.tif @09:17:39.54 SX HX9/16 (USB:03): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21jul2709h100ml70s.tif: TIFF Upload Complete @09:17:39.55 Gate.power :camera,:OFF @09:17:39.57 DAfans.email "48.474 sec auto-exposure",Subject: "da21jul2709h100ml images!" @09:17:39.83 Elbow.seek 1 @09:17:40.21 Forearm.seek 1 + 450 counts @09:17:40.29 SE.reconfigure :gently @09:17:40.34 SE.seek 1.56 Pucks below Top Plate @09:17:40.98 Elbow.reconfigure :limp @09:17:41.04 Forearm.reconfigure :loose @09:17:41.09 Forearm.seek 1 @09:17:41.31 Elbow.hold @09:17:41.39 Elbow.reconfigure :tight @09:17:41.44 SE.reconfigure :default @09:17:41.50 SE.seek 1.74 Pucks below Top Plate @09:17:41.90 Forearm.force -20 @09:17:41.94 Forearm.seek 1 + 311 counts @09:17:42.24 Forearm.reconfigure :tight @09:17:42.29 Forearm.seek 1 + 450 counts @09:17:42.59 SE.seek :down @09:17:43.79 Forearm.reconfigure :loose @09:17:43.84 Forearm.seek :retract @09:17:45.20 SE.stop @09:17:45.30 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @09:17:45.32
Starting finalPurge @09:17:45.51 Elbow.seek FlushPuck::Garage @09:17:47.36 Elbow.reconfigure :loose @09:17:47.55 Forearm.seek :garage @09:17:48.61 Hand.close @09:17:49.51 Forearm.seek :retract @09:17:50.54 Elbow.reconfigure :tight @09:17:50.64 Elbow.seek PC @09:17:52.04 Elbow.reconfigure :loose @09:17:52.25 Forearm.reconfigure :tight @09:17:52.33 Forearm.seek PC @09:17:53.88 PC.seek :closed @09:17:58.65 Processing Clamp onSpring/offSpring for 15/25 samples, on/off torque 1.71 @09:17:58.83 Hand.open @09:17:59.58 Forearm.reconfigure :loose @09:17:59.68 Forearm.seek :retract @09:18:00.69 PSR.select :air @09:18:00.73 PSV.dial PSR @09:18:01.35 Pulling 0.5ml of PSR.flush @09:18:01.37 PSR.select :flush @09:18:01.53 PS.seek 0.5ml @09:18:05.21
Waiting >20 seconds for psFlush to relinquish FlushPuck @09:18:07.63 delay 5 seconds @09:18:10.97 upsync @09:18:12.66 PSR.select :air @09:18:12.77 PS.seek 2.5ml @09:18:34.04 delay 1 second @09:18:35.06 PRV.dial :waste2,avoiding: :ab2 @09:18:36.93 PTV.dial :PRVmixing @09:18:38.25 PSV.dial :mixloop @09:18:39.29 PS.seek :empty @09:19:05.64 PSR.select :air @09:19:05.65 PSV.dial PSR @09:19:06.74 Pulling 0.5ml of PSR.flush @09:19:06.75 PSR.select :flush @09:19:06.86 PS.seek 0.5ml @09:19:12.86 delay 5 seconds @09:19:17.88 PSR.select :air @09:19:17.99 PS.seek 2.5ml @09:19:39.17 delay 1 second @09:19:40.19 PBV.dial :waste1 @09:19:40.56 PTV.dial :mixing @09:19:41.31 PSV.dial :mixloop @09:19:42.37 PS.seek :empty @09:20:08.92 PSV.dial PSR @09:20:09.98 PSR.select :flush @09:20:10.10 PS.seek 1ml @09:20:21.20 PSR.select :air @09:20:21.31 PS.seek 2.5ml @09:20:37.54 PSV.dial PTV @09:20:38.31 PTV.dial :puck @09:20:39.04 PBV.dial :waste1 @09:20:39.06 PS.seek 0ml @09:21:05.45 PSV.dial PSR @09:21:06.21 PSR.select :flush @09:21:06.33 PS.seek 0.5ml @09:21:12.23 PSR.select :air @09:21:12.34 PS.seek 2.5ml @09:21:33.54 PSV.dial PTV @09:21:34.43 PBV.dial PSV @09:21:35.01 PS.seek 0ml @09:22:01.29 PSV.dial PBV @09:22:01.75 PS.seek 2.5ml @09:22:28.16 PSV.dial :waste1 @09:22:28.92 PS.seek 0ml @09:22:55.29 PSV.dial PSR @09:22:55.76 PSR.select :air @09:22:55.78 PS.seek 2.5ml @09:23:22.13 PSV.dial PTV @09:23:22.85 PBV.dial PSV @09:23:22.87 PS.seek 0ml @09:23:49.16 PSV.dial PBV @09:23:49.64 PS.seek 2.5ml @09:24:16.04 PSV.dial :waste1 @09:24:16.79 PS.seek 0ml @09:24:43.13 PSV.dial PSR @09:24:43.60 PSR.select :flush @09:24:43.72 PS.seek 1ml @09:24:54.79 PSR.select :air @09:24:54.90 PS.seek 2.5ml @09:25:11.00 PSV.dial PTV @09:25:12.21 PTV.dial :puck @09:25:12.22 PBV.dial :waste1 @09:25:12.78 PS.seek 0ml @09:25:39.16 PSV.dial PSR @09:25:39.93 PSR.select :flush @09:25:40.04 PS.seek 0.5ml @09:25:46.03 PSR.select :air @09:25:46.15 PS.seek 2.5ml @09:26:07.38 PSV.dial PTV @09:26:08.15 PBV.dial PSV @09:26:08.71 PS.seek 0ml @09:26:35.25 PSV.dial PBV @09:26:35.73 PS.seek 2.5ml @09:27:02.10 PSV.dial :waste1 @09:27:02.84 PS.seek 0ml @09:27:29.38 PSV.dial PSR @09:27:29.85 PSR.select :air @09:27:29.87 PS.seek 2.5ml @09:27:56.28 PSV.dial PTV @09:27:57.14 PBV.dial PSV @09:27:57.16 PS.seek 0ml @09:28:23.50 PSV.dial PBV @09:28:23.96 PS.seek 2.5ml @09:28:50.28 PSV.dial :waste1 @09:28:51.02 PS.seek 0ml @09:29:17.44
Loading FlushPuck into Collection Clamp from Processing Clamp @09:29:17.56 Elbow.reconfigure :tight @09:29:17.60 Elbow.seek PC @09:29:17.71 Elbow.reconfigure :loose @09:29:17.80 Forearm.seek PC @09:29:18.76 Hand.close @09:29:19.52 PC.seek :open @09:29:24.24 Forearm.seek :retract @09:29:25.19 Elbow.reconfigure :tight @09:29:25.23 Elbow.seek CC @09:29:28.77 Elbow.reconfigure :loose @09:29:28.86 Forearm.reconfigure :tight @09:29:28.90 Forearm.seek CC @09:29:30.06 CC.seek :closed @09:29:34.61 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.33 @09:29:34.68 Hand.open @09:29:35.33 Forearm.reconfigure :loose @09:29:35.37 Forearm.seek :retract @09:29:36.36 Flushing the Collection Valve::Manifold @09:29:36.48 CSV.dial CSR @09:29:36.97 Pulling 5ml of CSR.flush CSR.select :flush @09:29:37.08 CS.seek 5ml @09:29:50.82 delay 5 seconds @09:29:55.84 CSR.select :air @09:29:55.98 CS.reconfigure :fast @09:29:56.02 CS.seek 7.501ml @09:30:02.82 CSV.dial :waste @09:30:04.10 CS.reconfigure :default @09:30:04.15 CS.seek 0ml @09:30:24.13 Flushing lower manifold's waste1 port @09:30:24.24 CSV.dial :flush @09:30:24.70 CS.reconfigure :fast @09:30:24.74 CS.seek 5ml @09:30:37.47 delay 5 seconds @09:30:42.49 CSV.dial :air @09:30:43.00 CS.seek 7.001ml @09:30:48.51 CSV.dial CTV @09:30:48.53
CTV.dial CSV @09:30:49.94 CBV.dial :waste1 @09:30:50.39 CS.seek 0ml @09:31:07.26 Flushing lower manifold's waste2 port @09:31:07.37 CSV.dial :flush @09:31:08.09 CS.seek 5ml @09:31:21.04 delay 5 seconds @09:31:26.05 CSV.dial :air @09:31:26.57 CS.seek 7ml @09:31:32.19 CSV.dial CTV @09:31:32.24
CTV.dial CSV @09:31:32.77 CBV.dial :waste2 @09:31:33.82 CS.seek 0ml @09:31:50.50 Disinfecting external Intake with kill @09:31:50.62 CSV.dial CSR @09:31:51.70 Pulling 8ml of CSR.kill @09:31:51.71 CSR.select :kill @09:31:51.82 CS.seek 8ml @09:32:11.72 delay 5 seconds @09:32:16.74 CSR.select :air @09:32:16.85 CS.seek 10ml @09:32:22.60 CSV.dial CBV @09:32:22.62
CBV.dial CSV @09:32:24.00 CTV.dial :intake @09:32:24.77 Intake.select :open @09:32:24.88 CS.seek 2ml @09:32:43.84 Intake.select :close @09:32:43.94 CTV.dial :air @09:32:44.73 CS.reconfigure :default @09:32:44.77 CS.seek 0ml @09:32:50.66 CSV.dial CSR @09:32:51.73 Pulling 5ml of CSR.flush @09:32:51.74 CSR.select :flush @09:32:51.85 CS.seek 5ml @09:33:05.54 delay 5 seconds @09:33:10.55 CSR.select :air @09:33:10.66 CS.seek 10ml @09:33:24.19 CSV.dial CBV @09:33:25.34 CS.seek 0ml @09:33:51.72 Garaging FlushPuck in Collection Clamp @09:33:51.79 Elbow.reconfigure :tight @09:33:51.83 Elbow.seek CC @09:33:51.94 Elbow.reconfigure :loose @09:33:52.02 Forearm.seek CC @09:33:52.98 Hand.close @09:33:53.64 CC.seek :open @09:33:58.27 Forearm.seek :retract @09:33:59.23 Elbow.reconfigure :tight @09:33:59.27 Elbow.seek :garage @09:34:02.17 Elbow.reconfigure :loose @09:34:02.26 Forearm.reconfigure :tight @09:34:02.30 Forearm.seek :garage @09:34:03.33 Hand.open @09:34:03.94 Forearm.reconfigure :loose @09:34:03.99 Forearm.seek :retract @09:34:04.93 Completed [initialPurge; da #da and wsr are run simultanelously] begun 07:40:50 @09:34:04.98 Asleep after completing seawater_negative_test_72721 assays @09:34:05.00 GoodNews.email "{1 => 3,\n 2 => 0,\n 3 => 0,\n 4 => 0,\n 5 => 0,\n 6 => 0,\n 7 => 0}\nCTD data unavailable\nCan@09:30:01, 16% flow, 23.0C, 41% humidity, 14.9psia, 13.447V, 0.447A, 0.429A avg, 6.02W\nSampled 100.0ml",Subject: "Asleep after completing seawater_negative_test_72721 assays" @09:34:23.45 upsync @13:34:35.77UTC08-Nov-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:34:38.40 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:34:42.16 Polling Can every 10:00 @13:34:42.17 Cleanly exited at 13:32:03UTC08-Nov-21 @13:34:42.20 Loading /home/esp/esp2/mission/DA_WCR_110821.rb mission @13:34:42.25 GoodNews.email "Starting DA_WCR_110821 w/pucks in tube 2\n{}\nCTD data unavailable\nCan@13:34:42, 16% flow, 20.7C, 13% humidity, 15.1psia, 13.510V, 0.309A, 0.290A avg, 4.17W",Subject: "DA_WCR_110821 begun" @13:34:42.63
Preparing to sleep until + 00:00:05 @13:34:42.66 Asleep til + 00:00:05 @13:34:42.68 Continuing at 13:34 @13:34:42.96 Gate.power :raw,:OFF @13:34:42.97 Gate.power :core,:OFF @13:34:42.98 Shutdown @13:34:45.95 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110821.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:34:42, 16% flow, 20.7C, 13% humidity, 15.1psia, 13.510V, 0.309A, 0.290A avg, 4.17W",Subject: "Asleep until 13:34" @13:34:47.24
Powering core bus @13:34:52.92 Gate.power :raw,:ON @13:34:54.08 CS.configure :default @13:34:54.26 PS.configure :default @13:34:54.50 SS.configure :default @13:34:54.67 CC.configure :default @13:34:54.85 PC.configure :default @13:34:55.09 Forearm.configure :loose @13:34:55.26 Elbow.configure :loose @13:34:55.47 SC.configure :loose @13:34:55.64 SE.configure :default @13:34:55.73 CH.coast @13:34:55.81 CH.configure :default @13:34:55.87 PH.coast @13:34:56.04 PH.configure :default @13:34:56.14 All dwarf controllers running firmware version 3.85 @13:34:56.28 Valve.reset @13:34:56.64 Intake.select :close @13:34:56.73 Exhaust.select :close @13:34:56.80 CTV.dial :air @13:34:56.95 PSV.dial :air @13:34:57.10 CSV.dial :waste1 @13:34:57.30
Forearm.reconfigure :loose @13:34:57.34 PS.reconfigure :default @13:34:57.48
Forearm.home.seek :retract @13:34:57.66 PS.home.seek 0ml @13:34:57.82 SSV.dial :spit,avoiding: :exhaust @13:34:58.08 CS.reconfigure :fast @13:34:58.13 SS.reconfigure :default @13:34:58.36 SS.home.seek 0ml @13:34:58.41 CS.home.seek 0ml @13:34:58.58 Hand.close @13:34:58.64
Elbow.jog 1500 @13:34:59.25 Elbow.reconfigure :loose @13:34:59.35 Elbow.home.seek :clear @13:35:02.66 SE.reconfigure :default @13:35:02.77 SE.home.seek :down @13:35:03.46 SC.reconfigure :tight @13:35:03.54 SC.home.seek :initial @13:35:06.03 PS.stop @13:35:22.57
SC.configure :limp @13:35:22.70 Forearm.seek :retract @13:35:22.86 Elbow.seek :hammer @13:35:24.00 Forearm.reconfigure :tight @13:35:24.08 Forearm.seek retracted @13:35:24.55 CC.reconfigure :default @13:35:24.57 PC.reconfigure :default @13:35:24.83 PC.home.seek :open @13:35:24.88
CC.home.seek :open @13:35:28.97 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @13:35:28.99 Notice.email "At 13:35:28 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110821.rb:14\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:35:28, 16% flow, 20.8C, 13% humidity, 15.1psia, 13.496V, 0.339A, 0.321A avg, 4.57W",Subject: "Started DA_WCR_110821 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @13:35:29.02
Starting initialPurge @13:35:29.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:35:29.24 Hand.open @13:35:30.17 Elbow.reconfigure :tight @13:35:30.29 Elbow.seek FlushPuck::Garage @13:35:32.23 Elbow.reconfigure :loose @13:35:32.40 Forearm.reconfigure :loose @13:35:32.58 Forearm.seek :garage @13:35:33.58 Hand.close @13:35:34.45 Forearm.seek :retract @13:35:35.48 Elbow.reconfigure :tight @13:35:35.60 Elbow.seek CC @13:35:38.62 Elbow.reconfigure :loose @13:35:38.85 Forearm.reconfigure :tight @13:35:38.92 Forearm.seek CC @13:35:40.58 CC.seek :closed @13:35:45.26 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.30 @13:35:45.45 Hand.open @13:35:46.22 Forearm.reconfigure :loose @13:35:46.29 Forearm.seek :retract @13:35:47.30 Flushing the Collection Valve::Manifold @13:35:47.57 CSV.dial CSR @13:35:49.18 Pulling 5ml of CSR.flush @13:35:49.19 CSR.select :flush @13:35:49.36 CS.reconfigure :default @13:35:49.47 CS.seek 5ml @13:36:03.10 delay 5 seconds @13:36:08.12 CSR.select :air @13:36:08.36 CS.reconfigure :fast @13:36:08.44 CS.seek 7.5ml @13:36:15.37 CSV.dial :waste @13:36:16.72 CS.reconfigure :default @13:36:16.79 CS.seek 0ml @13:36:36.89 Purging external Intake @13:36:37.11 CSV.dial :flush @13:36:37.61 CS.reconfigure :fast @13:36:37.68 CS.seek 10ml @13:37:02.77 delay 5 seconds @13:37:07.67 upsync @13:37:07.79 CSV.dial CBV @13:37:07.82
CBV.dial CSV @13:37:08.57 CTV.dial :intake @13:37:09.35 Intake.select :open @13:37:09.46 CS.seek 0ml @13:37:33.59 Intake.select :close @13:37:33.69 CTV.dial :air @13:37:34.50 Flushing upper manifold @13:37:34.61 CSV.dial :flush @13:37:35.86 CS.seek 5ml @13:37:48.84 delay 5 seconds @13:37:53.85 CSV.dial :air @13:37:54.37 CS.seek 7.001ml @13:38:00.06 CSV.dial CBV @13:38:00.08
CBV.dial CSV @13:38:01.14 CTV.dial :air @13:38:01.15 CS.seek 0ml @13:38:18.24 delay 7 seconds @13:38:25.27 CS.seek 3ml @13:38:33.43 CSV.dial :waste @13:38:33.89 CS.seek 0ml @13:38:41.97 CSV.dial :flush @13:38:42.42 CS.seek 3ml @13:38:50.68 delay 5 seconds @13:38:55.70 CSV.dial :air @13:38:56.21 CS.seek 5ml @13:39:02.09 CSV.dial :air @13:39:02.11 CS.seek 0ml @13:39:14.56 delay 7 seconds @13:39:21.58 CS.seek 3ml @13:39:29.74 CSV.dial :waste @13:39:30.48 CS.seek 0ml @13:39:38.56 Garaging FlushPuck in Collection Clamp @13:39:38.63 Elbow.reconfigure :tight @13:39:38.67 Elbow.seek CC @13:39:38.92 Elbow.reconfigure :loose @13:39:39.01 Forearm.seek CC @13:39:40.03 Hand.close @13:39:40.67 CC.seek :open @13:39:45.32 Forearm.seek :retract @13:39:46.31 Elbow.reconfigure :tight @13:39:46.35 Elbow.seek :garage @13:39:49.27 Elbow.reconfigure :loose @13:39:49.36 Forearm.reconfigure :tight @13:39:49.40 Forearm.seek :garage @13:39:50.44 Hand.open @13:39:51.45 Forearm.reconfigure :loose @13:39:51.56 Forearm.seek :retract @13:39:52.50 da sampling at most 100ml, wcr at most 100ml @13:39:52.53 Loading sh1 puck from tube 2 into Collection Clamp @13:39:52.55 DAfans.email "Started da in tube 2 at 13:39:52UTC08-Nov-21\n{1 => 0,\n 2 => 33,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:35:28, 16% flow, 20.8C, 13% humidity, 15.1psia, 13.496V, 0.339A, 0.321A avg, 4.57W",Subject: "da sampling at most 100ml, wcr at most 100ml" @13:39:52.79
SC.reconfigure :tight @13:39:52.86 SC.seek 2 @13:39:59.69 SC.configure :limp @13:40:00.20 Elbow.reconfigure :tight @13:40:00.28 Elbow.seek 2 @13:40:02.38 Forearm.reconfigure :tight @13:40:02.46 Forearm.seek 2 + 450 counts @13:40:03.69 SE.reconfigure :pick @13:40:03.79 SE.seek :beamBreak @13:40:04.85 SE.configure :default @13:40:05.22 Elbow.seek 2 @13:40:05.59 Forearm.seek 2 + 300 counts @13:40:06.07 Forearm.reconfigure :loose @13:40:06.23 Hand.close @13:40:06.84 SE.seek :down @13:40:07.31
Forearm.seek :retract @13:40:07.54 SE.stop @13:40:08.34
Elbow.seek CC @13:40:10.11 Elbow.reconfigure :loose @13:40:10.29 Forearm.reconfigure :tight @13:40:10.37 Forearm.seek CC @13:40:12.01 CC.seek :closed @13:40:16.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.30 @13:40:16.76 Hand.open @13:40:17.62 Forearm.reconfigure :loose @13:40:17.70 Forearm.seek :retract @13:40:18.67 collectSample 100ml w/bubble point of 24psi @13:40:18.69 Priming 7ml @13:40:18.71 Intake.select :close @13:40:18.73 Exhaust.select :close @13:40:18.75 SS.hold @13:40:19.17 Calibrating pressure and temperature sensors @13:40:19.23 CTV.dial :air @13:40:19.25 SSV.dial :spit,avoiding: :exhaust @13:40:19.29 SS.reconfigure :fast @13:40:19.37 SS.seek 5ml @13:40:22.52 SSV.dial CBV @13:40:22.63
CBV.dial SSV @13:40:23.97 delay 3.8 seconds @13:40:27.98 SSV.dial :spit @13:40:29.36 SS.seek 0ml @13:40:32.35 SSV.dial CBV @13:40:33.71 CTV.dial :intake @13:40:34.56 SSV.dial CBV @13:40:34.61
CBV.dial SSV @13:40:34.63 Allowing 40s for 7.0ml of remaining 7.0ml @13:40:34.65 Intake.select :open @13:40:36.97 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:40:37.00 SS.reconfigure :inhaling 2.20ml/s @13:40:37.09 SS.seek 7ml @13:40:43.30 Stroke completed -- waiting for pressure to equalize ... @13:40:43.32 SS.reconfigure :exhale @13:40:43.41 SS.hold @13:40:43.87 Intake.select :close @13:40:44.09 SSV.dial :exhaust @13:40:44.88 Exhaust.select :open @13:40:45.04 SS.seek 0ml @13:40:49.24 Exhaust.select :close @13:40:49.40 Sampling 100.0ml @13:40:49.42 Intake.select :close @13:40:49.44 Exhaust.select :close @13:40:49.50 SS.reconfigure :default @13:40:49.57 SS.hold @13:40:49.96 CTV.dial :intake @13:40:49.98 SSV.dial CBV @13:40:50.06
CBV.dial SSV @13:40:50.82 Allowing 130s for 25.0ml of remaining 100.0ml @13:40:50.84 Intake.select :open @13:40:53.17 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:40:53.20 SS.reconfigure :inhaling 2.20ml/s @13:40:53.29 SS.seek 25ml @13:41:04.36 Vacuum too high -- waiting up to 40 seconds @13:41:04.38 SS.reconfigure :exhale @13:41:04.46 SS.hold @13:41:05.24 Intake @10.8psia, vacuum from 5.3 to 8.3psi @13:41:05.27 SS.reconfigure :inhaling 1.98ml/s @13:41:05.36 SS.seek 25ml @13:41:09.01 Vacuum too high -- waiting up to 40 seconds @13:41:09.02 SS.reconfigure :exhale @13:41:09.11 SS.hold @13:41:15.63 Intake @12.6psia, vacuum from 7.1 to 10.1psi @13:41:15.66 SS.reconfigure :inhaling 1.79ml/s @13:41:15.75 SS.seek 25ml @13:41:18.71 Stroke completed -- waiting for pressure to equalize ... @13:41:18.73 SS.reconfigure :exhale @13:41:18.83 SS.hold @13:41:20.75 Intake.select :close @13:41:21.04 SSV.dial :exhaust @13:41:21.84 Exhaust.select :open @13:41:21.98 SS.seek 0ml @13:41:30.83 Exhaust.select :close @13:41:30.98 SSV.dial CBV @13:41:31.03
CBV.dial SSV @13:41:31.88 Allowing 130s for 25.0ml of remaining 75.0ml @13:41:31.90 Intake.select :open @13:41:34.15 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:41:34.18 SS.reconfigure :inhaling 1.79ml/s @13:41:34.27 SS.seek 25ml @13:41:48.81 Vacuum too high -- waiting up to 40 seconds @13:41:48.83 SS.reconfigure :exhale @13:41:48.92 SS.hold @13:41:49.63 Intake @11.0psia, vacuum from 5.5 to 8.5psi @13:41:49.65 SS.reconfigure :inhaling 1.61ml/s @13:41:49.74 SS.seek 25ml @13:41:53.68 Stroke completed -- waiting for pressure to equalize ... @13:41:53.70 SS.reconfigure :exhale @13:41:53.79 SS.hold @13:42:02.10 Intake.select :close @13:42:02.31 SSV.dial :exhaust @13:42:03.10 Exhaust.select :open @13:42:03.28 SS.seek 0ml @13:42:12.10 Exhaust.select :close @13:42:12.24 SSV.dial CBV @13:42:12.32
CBV.dial SSV @13:42:13.09 Allowing 130s for 25.0ml of remaining 50.0ml @13:42:13.11 Intake.select :open @13:42:15.38 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:42:15.41 SS.reconfigure :inhaling 1.61ml/s @13:42:15.50 SS.seek 25ml @13:42:20.46 upsync @13:42:33.21
Vacuum too high -- waiting up to 40 seconds @13:42:33.22 SS.reconfigure :exhale @13:42:33.26 SS.hold @13:42:33.78 Intake @11.1psia, vacuum from 5.6 to 8.6psi @13:42:33.79 SS.reconfigure :inhaling 1.45ml/s @13:42:33.84 SS.seek 25ml @13:42:36.00 Stroke completed -- waiting for pressure to equalize ... @13:42:36.01 SS.reconfigure :exhale @13:42:36.05 SS.hold @13:42:43.50 Intake.select :close @13:42:43.64 SSV.dial :exhaust @13:42:44.39 Exhaust.select :open @13:42:44.50 SS.seek 0ml @13:42:53.53 Exhaust.select :close @13:42:53.63 SSV.dial CBV @13:42:53.68
CBV.dial SSV @13:42:54.41 Allowing 130s for 25.0ml of remaining 25.0ml @13:42:54.42 Intake.select :open @13:42:56.57 Intake @14.3psia, vacuum from 8.8 to 11.8psi @13:42:56.58 SS.reconfigure :inhaling 1.45ml/s @13:42:56.63 SS.seek 24.99ml @13:43:16.76 Stroke completed -- waiting for pressure to equalize ... @13:43:16.77 SS.reconfigure :exhale @13:43:16.81 SS.hold @13:43:24.17 Intake.select :close @13:43:24.31 SSV.dial :exhaust @13:43:25.06 Exhaust.select :open @13:43:25.17 SS.seek 0ml @13:43:33.95 Exhaust.select :close @13:43:34.06 Sampled 100.0ml @13:43:34.07 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:43:34.08 Intake.select :close @13:43:34.14 CSV.dial :waste @13:43:34.20 CS.seek 0ml @13:43:34.27 SSV.dial CBV,avoiding: :exhaust @13:43:34.30
CBV.dial SSV @13:43:34.46 CSV.dial :air @13:43:35.20 CS.seek 10ml @13:43:36.22
CTV.dial :air @13:43:37.03 delay 15 seconds @13:43:52.05 SSV.dial :spit,avoiding: :exhaust @13:43:53.39 CTV.dial CSV,avoiding: :intake @13:43:53.42
SS.seek 0ml @13:43:53.53 SSV.dial CBV,avoiding: :exhaust @13:44:00.49 CBV.dial between SSV and waste2 @13:44:01.37 CTV.dial CSV,avoiding: :air @13:44:01.39
CSV.dial CTV @13:44:01.84 CS.reconfigure :pressurize @13:44:01.88 CS.seek 0ml @13:44:20.21 BubblePoint: Intake @39.4psia, Outlet @15.2psia @13:44:20.22 CBV.dial SSV @13:44:20.56 SS.reconfigure :default @13:44:20.60 SS.seek 10ml @13:44:26.34 delay 15 seconds @13:44:41.46 Evacuated: Intake @16.7psia, Outlet @9.8psia @13:44:41.47 SSV.dial :spit,avoiding: :exhaust @13:44:42.82 SS.reconfigure :exhale @13:44:42.86 SS.seek 0ml @13:44:48.31 SS.reconfigure :fast @13:44:48.35 SS.seek 5ml @13:44:51.30 SSV.dial CBV,avoiding: :exhaust @13:44:51.32
CBV.dial SSV @13:44:52.69 CTV.dial :air,avoiding: :intake @13:44:54.08 CSV.dial CTV @13:44:54.12
SS.seek 10ml @13:44:55.11 CSV.dial :air @13:44:55.57 CS.reconfigure :fast @13:44:55.61 CS.seek 0ml @13:44:56.56
SSV.dial :spit,avoiding: :exhaust @13:44:57.91 SS.reconfigure :exhale @13:44:57.95 SS.seek 0ml @13:45:03.15 SS.reconfigure :fast @13:45:03.19 SS.seek 5ml @13:45:03.64 CS.stop @13:45:06.13 SSV.dial CBV,avoiding: :exhaust @13:45:06.17
CBV.dial SSV @13:45:08.49 SSV.dial :spit,avoiding: :exhaust @13:45:09.82 SS.reconfigure :default @13:45:09.86 SS.seek 0ml @13:45:13.17 SS.stop @13:45:13.21 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:45:13.22 Intake.select :close @13:45:13.28 CSV.dial :waste @13:45:13.40 SSV.dial CBV,avoiding: :exhaust @13:45:13.42
CBV.dial SSV @13:45:14.04 CS.seek 0ml @13:45:14.27 CSV.dial :air @13:45:14.79
CTV.dial :air @13:45:14.80 delay 15 seconds @13:45:15.01 CS.seek 10ml @13:45:29.81
SSV.dial :spit,avoiding: :exhaust @13:45:31.14 CTV.dial CSV,avoiding: :intake @13:45:31.19
SS.reconfigure :exhale @13:45:31.26 SS.seek 0ml @13:45:31.66 SSV.dial CBV,avoiding: :exhaust @13:45:40.14 CBV.dial between SSV and waste2 @13:45:40.53 CTV.dial CSV,avoiding: :air @13:45:40.56
CSV.dial CTV @13:45:41.02 CS.reconfigure :pressurize @13:45:41.06 CS.seek 0ml @13:46:02.17 BubblePoint: Intake @38.4psia, Outlet @14.3psia @13:46:02.18 CBV.dial SSV @13:46:02.51 SS.reconfigure :default @13:46:02.55 SS.seek 10ml @13:46:08.25 delay 15 seconds @13:46:23.36 Evacuated: Intake @18.6psia, Outlet @11.8psia @13:46:23.37 SSV.dial :spit,avoiding: :exhaust @13:46:24.72 SS.reconfigure :exhale @13:46:24.76 SS.seek 0ml @13:46:30.15 SS.reconfigure :fast @13:46:30.19 SS.seek 5ml @13:46:33.14 SSV.dial CBV,avoiding: :exhaust @13:46:33.16
CBV.dial SSV @13:46:34.52 CTV.dial :air,avoiding: :intake @13:46:35.91 CSV.dial CTV @13:46:35.95
SS.seek 10ml @13:46:36.94 CSV.dial :air @13:46:37.41 CS.reconfigure :fast @13:46:37.46 CS.seek 0ml @13:46:38.42
SSV.dial :spit,avoiding: :exhaust @13:46:39.77 SS.reconfigure :exhale @13:46:39.81 SS.seek 0ml @13:46:43.05 CS.stop @13:46:44.68
SS.reconfigure :fast @13:46:44.72 SS.seek 5ml @13:46:47.66 SSV.dial CBV,avoiding: :exhaust @13:46:47.68
CBV.dial SSV @13:46:50.04 SSV.dial :spit,avoiding: :exhaust @13:46:51.38 SS.reconfigure :default @13:46:51.42 SS.seek 0ml @13:46:54.72 SS.stop @13:46:54.75 Lysing: Adding 2.0ml AqMeOH @13:46:54.77 CBV.dial between CSV and waste1,avoiding: :DBV @13:46:56.27 CTV.dial CSV @13:46:57.63 CSV.dial CSR @13:46:58.41 Pulling 1ml of CSR.AqMeOH @13:46:58.42 CSR.select :AqMeOH @13:46:58.53 CS.reconfigure :default @13:46:58.57 CS.seek 1ml @13:47:02.05 delay 5 seconds @13:47:07.07 CSR.select :air @13:47:07.22 CS.seek 6ml @13:47:20.89 delay 2 seconds @13:47:22.91 CSV.dial :waste2 @13:47:23.46 CS.reconfigure :fast @13:47:23.50 CS.seek 0ml @13:47:38.39 CSV.dial CSR @13:47:38.99 Pulling 2ml of CSR.AqMeOH @13:47:39.00 CSR.select :AqMeOH @13:47:39.10 CS.reconfigure :default @13:47:39.15 CS.seek 2ml @13:47:45.11 delay 5 seconds @13:47:50.13 CSR.select :air @13:47:50.28 CS.seek 5.001ml @13:47:58.73 delay 2 seconds @13:48:00.75 CSV.dial CTV @13:48:01.21 CS.seek 1ml @13:48:12.56 Before lysing Collection Puck Heater at 20.1C/39.0psia @13:48:12.58 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:48:12.59 CH.seek 70C,8:00 @13:50:27.04 CH.hold 10:00,70C @13:50:27.23 Begun lysing Collection Puck Heater at 68.1C/45.1psia @14:00:27.23 Ended lysing Collection Puck Heater at 70C/45.4psia @14:00:27.25 CS.seek 0ml @14:00:30.85 CTV.dial between spare and air,avoiding: :intake @14:00:31.91 CSV.dial :air @14:00:32.36 CS.seek 5ml @14:00:45.94 CSV.dial CTV @14:00:46.38 delay 2 seconds @14:00:48.39 CSV.dial :air @14:00:48.85 delay 2 seconds @14:00:50.86 CSV.dial CTV @14:00:51.32 delay 2 seconds @14:00:53.33 CSV.dial :air @14:00:53.78 delay 2 seconds @14:00:55.81 CS.seek 0ml @14:01:09.55 Puck @73.4psia after final lysis pressurization @14:01:09.56 Recovering Lysate into Collection syringe @14:01:09.57 CSV.dial CBV @14:01:10.60 CBV.dial CSV @14:01:10.95 CS.seek 5ml @14:01:24.50 delay 5 seconds @14:01:29.52 CTV.dial :air @14:01:30.04 CS.seek 7ml @14:01:36.00 delay 5 seconds @14:01:41.02 CSV.dial :air2 @14:01:41.46 CS.seek 10ml @14:01:50.13 CSV.dial between air and CTV,avoiding: CBV @14:01:51.41 Lysing Complete @14:01:51.48 Storing sh1 puck in Collection Clamp to tube 1 @14:01:51.64 SC.reconfigure :tight @14:01:51.68 SC.seek 1 @14:01:55.32 SC.configure :limp @14:01:55.46 SE.reconfigure :place @14:01:55.52
Elbow.reconfigure :tight @14:01:55.56 SE.seek :beamBreak @14:01:55.59
Elbow.seek CC @14:01:56.03 Elbow.reconfigure :loose @14:01:56.12 Forearm.seek CC @14:01:57.10 Hand.close @14:01:57.65 CC.seek :open @14:01:58.44 SE.configure :default @14:01:58.49 SE.seek :place @14:02:02.40
Forearm.seek :retract @14:02:03.34 Elbow.reconfigure :tight @14:02:03.38 Elbow.seek 1 @14:02:05.07 Elbow.reconfigure :limp @14:02:05.11 Forearm.seek 1 @14:02:06.10 Elbow.hold @14:02:06.16 Elbow.reconfigure :tight @14:02:06.20 Hand.open @14:02:07.39 Forearm.force -20 @14:02:07.43 Forearm.seek 1 + 315 counts @14:02:07.72 Forearm.reconfigure :tight @14:02:07.81 Forearm.seek 1 + 450 counts @14:02:08.11 SE.seek :down @14:02:09.23
Forearm.reconfigure :loose @14:02:09.27 Forearm.seek :retract @14:02:10.80 SE.stop @14:02:10.84
Tranferring DA @14:02:10.85 Beginning samplePassC @14:02:10.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:02:11.04 Elbow.seek FlushPuck::Garage @14:02:12.83 Elbow.reconfigure :loose @14:02:12.92 Forearm.seek :garage @14:02:13.96 Hand.close @14:02:14.68 Forearm.seek :retract @14:02:15.70 Elbow.reconfigure :tight @14:02:15.74 Elbow.seek CC @14:02:18.64 Elbow.reconfigure :loose @14:02:18.73 Forearm.reconfigure :tight @14:02:18.77 Forearm.seek CC @14:02:20.03 CC.seek :closed @14:02:24.56 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @14:02:24.62 Hand.open @14:02:25.33 Forearm.reconfigure :loose @14:02:25.38 Forearm.seek :retract @14:02:26.38 PSV.dial PSR @14:02:27.79 Pulling 1.5ml of PSR.flush PSR.select :flush @14:02:27.91 PS.seek 1.5ml @14:02:44.11 delay 5 seconds @14:02:49.13 PSV.dial :waste1 @14:02:49.58 PS.seek 0ml @14:03:05.73 PSV.dial PSR @14:03:06.18 PSR.select :air @14:03:06.29 PS.seek 2.5ml @14:03:32.61 PRV.dial CBV,avoiding: :ab2 @14:03:32.63
CBV.dial PRV @14:03:34.34 PSV.dial :mixloop @14:03:35.51 CTV.dial :air,avoiding: :intake @14:03:35.52 PTV.dial :PRVmixing @14:03:36.84 PS.seek 0ml @14:04:03.20 PSV.dial :air @14:04:03.67 PS.seek 2.5ml @14:04:29.95 PSV.dial :mixloop @14:04:30.42 PS.seek 0ml @14:04:56.70 PSV.dial :air @14:04:57.28 PS.seek 2.5ml @14:05:23.66 PSV.dial :mixloop @14:05:24.11 PS.seek 0ml @14:05:50.48 Garaging FlushPuck in Collection Clamp @14:05:50.55 Elbow.reconfigure :tight @14:05:50.59 Elbow.seek CC @14:05:50.70 Elbow.reconfigure :loose @14:05:50.79 Forearm.seek CC @14:05:51.72 Hand.close @14:05:52.27 CC.seek :open @14:05:56.92 Forearm.seek :retract @14:05:57.91 Elbow.reconfigure :tight @14:05:57.96 Elbow.seek :garage @14:06:00.83 Elbow.reconfigure :loose @14:06:00.91 Forearm.reconfigure :tight @14:06:00.95 Forearm.seek :garage @14:06:01.90 Hand.open @14:06:02.58 Forearm.reconfigure :loose @14:06:02.62 Forearm.seek :retract @14:06:03.57 PSV.dial :mixloop @14:06:03.58 PTV.dial :PRVmixing @14:06:03.59 PRV.dial CBV @14:06:03.62
CBV.dial PRV @14:06:04.09 CTV.dial :air,avoiding: :intake @14:06:04.12 Loading lyfil puck from tube 2 into Collection Clamp @14:06:04.33 SC.reconfigure :tight @14:06:04.38 SC.seek 2 @14:06:07.92 SC.configure :limp @14:06:08.11 Elbow.reconfigure :tight @14:06:08.15 Elbow.seek 2 @14:06:10.04 Forearm.reconfigure :tight @14:06:10.08 Forearm.seek 2 + 450 counts @14:06:11.10 SE.reconfigure :pick @14:06:11.15 SE.seek :beamBreak @14:06:12.22 SE.configure :default @14:06:12.36 Elbow.seek 2 @14:06:12.53 Forearm.seek 2 + 300 counts @14:06:13.01 Forearm.reconfigure :loose @14:06:13.05 Hand.close @14:06:13.66 SE.seek :down @14:06:13.85
Forearm.seek :retract @14:06:14.37 SE.stop @14:06:14.84
Elbow.seek CC @14:06:16.49 Elbow.reconfigure :loose @14:06:16.58 Forearm.reconfigure :tight @14:06:16.62 Forearm.seek CC @14:06:17.81 CC.seek :closed @14:06:22.34 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.15 @14:06:22.35 Hand.open @14:06:22.96 Forearm.reconfigure :loose @14:06:23.00 Forearm.seek :retract @14:06:24.01 CTV.dial CSV,avoiding: :intake @14:06:25.42 CSV.dial CTV @14:06:25.71 PS.seek 2.5ml @14:06:52.13 CS.seek 4.5ml @14:07:07.06 delay 8 seconds @14:07:15.08 PSV.dial :air @14:07:15.54 delay 2 seconds @14:07:17.55 PSV.dial :waste2 @14:07:18.31 PS.seek 0ml @14:07:44.69 CBV.dial between PRV and waste1 @14:07:45.03 CSV.dial :air @14:07:45.49 CS.seek 10ml @14:08:00.43 CSV.dial CTV @14:08:00.88 CS.seek 0ml @14:08:27.65 CBV.dial :waste1 @14:08:27.93 delay 5 seconds @14:08:32.95 CTV.dial :air,avoiding: :intake @14:08:34.38 Storing lyfil puck in Collection Clamp to tube 1 @14:08:34.54 SC.reconfigure :tight @14:08:34.58 SC.seek 1 @14:08:38.19 SC.configure :limp @14:08:38.38 Elbow.reconfigure :tight @14:08:38.42 Elbow.seek 1 @14:08:40.11 Forearm.reconfigure :tight @14:08:40.16 Forearm.seek 1 + 450 counts @14:08:41.30 SE.reconfigure :pick @14:08:41.35 SE.seek :beamBreak @14:08:44.37 SE.configure :default @14:08:44.51 Elbow.reconfigure :limp @14:08:44.56 Forearm.reconfigure :loose @14:08:44.60 Forearm.seek 1 @14:08:44.83 Elbow.hold @14:08:44.89 Elbow.reconfigure :tight @14:08:44.98 SE.seek 0.18 Pucks below Top Plate @14:08:45.87 Forearm.force -20 @14:08:45.91 Forearm.seek 1 + 314 counts @14:08:46.20 Forearm.reconfigure :tight @14:08:46.24 Forearm.seek 1 + 450 counts @14:08:46.45 SE.seek 1.18 Pucks below Top Plate @14:08:46.95 Forearm.reconfigure :loose @14:08:46.99 Forearm.seek :retract @14:08:47.91 Elbow.seek CC @14:08:49.61 Elbow.reconfigure :loose @14:08:49.70 Forearm.seek CC @14:08:50.68 Hand.close @14:08:51.27 CC.seek :open @14:08:55.99 Forearm.seek :retract @14:08:57.01 Elbow.reconfigure :tight @14:08:57.05 Elbow.seek 1 @14:08:58.80 Elbow.reconfigure :limp @14:08:58.85 Forearm.seek 1 @14:08:59.80 Elbow.hold @14:08:59.86 Elbow.reconfigure :tight @14:08:59.90 Hand.open @14:09:00.63 Forearm.force -20 @14:09:00.66 Forearm.seek 1 + 295 counts @14:09:00.96 Forearm.reconfigure :tight @14:09:01.00 Forearm.seek 1 + 450 counts @14:09:01.27 SE.seek :down @14:09:02.40
Forearm.reconfigure :loose @14:09:02.44 Forearm.seek :retract @14:09:03.89 SE.stop @14:09:03.92
PSV.dial :air @14:09:04.79 PS.seek 2.5ml @14:09:31.19 delay 1 second @14:09:32.20 PSV.dial :mixloop @14:09:32.66 delay 1 second @14:09:33.67 PSV.dial :air @14:09:34.25 delay 1 second @14:09:35.27 PSV.dial :mixloop @14:09:35.71 delay 1 second @14:09:36.72 PSV.dial :air @14:09:37.19 delay 1 second @14:09:38.21 PS.seek 0ml @14:10:04.63 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:10:04.80 Elbow.seek FlushPuck::Garage @14:10:06.59 Elbow.reconfigure :loose @14:10:06.67 Forearm.seek :garage @14:10:07.67 Hand.close @14:10:08.38 Forearm.seek :retract @14:10:09.38 Elbow.reconfigure :tight @14:10:09.42 Elbow.seek PC @14:10:10.67 Elbow.reconfigure :loose @14:10:10.76 Forearm.reconfigure :tight @14:10:10.80 Forearm.seek PC @14:10:12.10 PC.seek :closed @14:10:16.77 Processing Clamp onSpring/offSpring for 14/26 samples, on/off torque 1.61 @14:10:16.84 Hand.open @14:10:17.45 Forearm.reconfigure :loose @14:10:17.49 Forearm.seek :retract @14:10:18.45 PRV.dial :air @14:10:18.89 PSV.dial :mixloop @14:10:19.41 PS.seek 0.3ml @14:10:23.25 PSV.dial PSR @14:10:24.35 Pulling 0.4ml of PSR.air @14:10:24.36 PSR.select :air @14:10:24.39 PS.seek 0.7ml @14:10:29.85 delay 3 seconds @14:10:32.86 PSR.select :air @14:10:32.88 PS.seek 2.5ml @14:10:52.13 delay 3 seconds @14:10:55.14 PSV.dial :mixloop @14:10:56.33 PTV.dial :mixing @14:10:57.06 PBV.dial :waste2 @14:10:58.01 PS.seek 0ml @14:11:24.32 Garaging FlushPuck in Processing Clamp @14:11:24.39 Elbow.reconfigure :tight @14:11:24.43 Elbow.seek PC @14:11:24.54 Elbow.reconfigure :loose @14:11:24.63 Forearm.seek PC @14:11:25.59 Hand.close @14:11:26.15 PC.seek :open @14:11:30.89 Forearm.seek :retract @14:11:31.88 Elbow.reconfigure :tight @14:11:31.92 Elbow.seek :garage @14:11:33.26 Elbow.reconfigure :loose @14:11:33.35 Forearm.reconfigure :tight @14:11:33.39 Forearm.seek :garage @14:11:34.34 Hand.open @14:11:34.95 Forearm.reconfigure :loose @14:11:34.99 Forearm.seek :retract @14:11:35.99 Loading da puck from tube 2 into Processing Clamp @14:11:36.20 SC.reconfigure :tight @14:11:36.25 SC.seek 2 @14:11:39.81 SC.configure :limp @14:11:40.00 Elbow.reconfigure :tight @14:11:40.04 Elbow.seek 2 @14:11:41.88 Forearm.reconfigure :tight @14:11:41.93 Forearm.seek 2 + 450 counts @14:11:42.95 SE.reconfigure :pick @14:11:43.00 SE.seek :beamBreak @14:11:44.14 SE.configure :default @14:11:44.28 Elbow.seek 2 @14:11:44.44 Forearm.seek 2 + 300 counts @14:11:44.64 Forearm.reconfigure :loose @14:11:44.68 Hand.close @14:11:45.28 SE.seek :down @14:11:45.46
Forearm.seek :retract @14:11:46.01 SE.stop @14:11:46.40
Elbow.seek PC @14:11:48.91 Elbow.reconfigure :loose @14:11:49.00 Forearm.reconfigure :tight @14:11:49.04 Forearm.seek PC @14:11:50.23 PC.seek :closed @14:11:54.85 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @14:11:54.86 Hand.open @14:11:55.58 Forearm.reconfigure :loose @14:11:55.62 Forearm.seek :retract @14:11:56.62 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:11:56.64 PH.seek 28C,5:00 @14:12:45.88 PH.hold 2:00:00,28C @14:12:45.93 delay 1:30 @14:14:16.45 PSR.select :air @14:14:16.46 PSV.dial PSR @14:14:17.55 Pulling 0.5ml of PSR.wash @14:14:17.56 PSR.select :wash @14:14:17.68 PS.seek 0.5ml @14:14:23.66 delay 5 seconds @14:14:28.67 PSR.select :air @14:14:28.79 PS.seek 2ml @14:14:44.97 PTV.dial :puck @14:14:45.70 PSV.dial PTV @14:14:46.47 PBV.dial :waste1 @14:14:47.87 PS.seek 1.5ml @14:14:53.87 delay 3 seconds @14:14:56.89 PBV.dial between PSV and waste1 @14:14:57.26 PS.seek 0ml @14:15:13.59 delay 5:00 @14:20:13.62 recoverPSRreagent08 PTV.dial :puck @14:20:13.63 PBV.dial :waste1 @14:20:14.02 delay 30 seconds @14:20:44.05 PBV.dial between PSV and waste1 @14:20:44.41 PSR.select :air @14:20:44.43 PSV.dial PSR @14:20:45.20 PS.reconfigure :fast @14:20:45.24 PS.seek 2.5ml @14:21:09.52 PSV.dial PTV @14:21:10.29 delay 5 seconds @14:21:15.31 PSV.dial PSR @14:21:16.07 delay 5 seconds @14:21:21.09 PSV.dial PTV @14:21:21.97 delay 5 seconds @14:21:26.99 PSV.dial :air @14:21:27.78 PS.seek 0ml @14:21:51.83 PTV.dial :PRVmixing,via: PRV @14:21:53.16 PSV.dial :mixloop @14:21:53.63 PRV.dial :ab1 @14:21:54.37 PS.reconfigure :slow @14:21:54.41 PS.seek 0.15ml @14:21:57.96 delay 2 seconds @14:21:59.98 CBV.dial PRV @14:22:00.00
PRV.dial CBV @14:22:00.58 PS.seek 0.35ml @14:22:05.18 delay 2 seconds @14:22:07.19 CBV.dial between PRV and PSV @14:22:07.50 PRV.dial :air @14:22:07.95 PS.seek 0.9ml @14:22:19.74 PRV.dial :waste2 @14:22:20.48 PS.seek 0.5ml @14:22:29.18 addPMLReagent08 @14:22:29.19 PSV.dial :air @14:22:29.65 PS.reconfigure :default @14:22:29.69 PS.seek 2ml @14:22:45.86 PSV.dial :PML @14:22:46.32 PTV.dial :mixing @14:22:47.07 PBV.dial :waste1 @14:22:47.49 PS.seek 1.85ml @14:22:49.83 PBV.dial between PSV and waste1 @14:22:50.21 PS.seek 0ml @14:23:09.92 delay 2 seconds @14:23:11.94 Beginning samplePassC @14:23:11.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:23:12.11 Elbow.reconfigure :tight @14:23:12.15 Elbow.seek FlushPuck::Garage @14:23:13.50 Elbow.reconfigure :loose @14:23:13.59 Forearm.seek :garage @14:23:14.56 Hand.close @14:23:15.25 Forearm.seek :retract @14:23:16.26 Elbow.reconfigure :tight @14:23:16.30 Elbow.seek CC @14:23:19.11 Elbow.reconfigure :loose @14:23:19.19 Forearm.reconfigure :tight @14:23:19.24 Forearm.seek CC @14:23:20.44 CC.seek :closed @14:23:24.99 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.52 @14:23:25.07 Hand.open @14:23:25.70 Forearm.reconfigure :loose @14:23:26.20 Forearm.seek :retract @14:23:27.19 PSV.dial PSR @14:23:28.32 Pulling 1.5ml of PSR.flush @14:23:28.33 PSR.select :flush @14:23:28.45 PS.seek 1.5ml @14:23:44.55 delay 5 seconds @14:23:49.57 PSV.dial :waste1 @14:23:50.02 PS.seek 0ml @14:24:06.14 PSV.dial PSR @14:24:06.61 PSR.select :air @14:24:06.72 PS.seek 2.5ml @14:24:33.12 PRV.dial CBV,avoiding: :ab2 @14:24:33.14
CBV.dial PRV @14:24:34.78 PSV.dial :mixloop @14:24:35.83 CTV.dial :air,avoiding: :intake @14:24:35.84 PTV.dial :PRVmixing @14:24:36.60 PS.seek 0ml @14:25:02.92 PSV.dial :air @14:25:03.38 PS.seek 2.5ml @14:25:29.70 PSV.dial :mixloop @14:25:30.16 PS.seek 0ml @14:25:56.51 PSV.dial :air @14:25:56.97 PS.seek 2.5ml @14:26:23.36 PSV.dial :mixloop @14:26:23.83 PS.seek 0ml @14:26:50.39 Garaging FlushPuck in Collection Clamp @14:26:50.46 Elbow.reconfigure :tight @14:26:50.50 Elbow.seek CC @14:26:50.78 Elbow.reconfigure :loose @14:26:50.87 Forearm.seek CC @14:26:51.85 Hand.close @14:26:52.51 CC.seek :open @14:26:57.15 Forearm.seek :retract @14:26:58.12 Elbow.reconfigure :tight @14:26:58.16 Elbow.seek :garage @14:27:01.06 Elbow.reconfigure :loose @14:27:01.15 Forearm.reconfigure :tight @14:27:01.19 Forearm.seek :garage @14:27:02.21 Hand.open @14:27:02.94 Forearm.reconfigure :loose @14:27:02.98 Forearm.seek :retract @14:27:03.98 delayUntil "14:31:11.93" @14:27:04.00 Whole Cell Archive sampling 100ml @14:27:04.02 Loading wcr puck from tube 2 into Collection Clamp @14:27:04.46 Elbow.reconfigure :tight @14:27:04.50 Elbow.seek 2 @14:27:06.40 Forearm.reconfigure :tight @14:27:06.45 Forearm.seek 2 + 450 counts @14:27:07.48 SE.reconfigure :pick @14:27:07.53 SE.seek :beamBreak @14:27:08.70 SE.configure :default @14:27:08.85 Elbow.seek 2 @14:27:09.02 Forearm.seek 2 + 300 counts @14:27:09.21 Forearm.reconfigure :loose @14:27:09.25 Hand.close @14:27:09.90 SE.seek :down @14:27:10.08 Forearm.seek :retract @14:27:10.72 SE.stop @14:27:11.09 Elbow.seek CC @14:27:12.75 Elbow.reconfigure :loose @14:27:12.85 Forearm.reconfigure :tight @14:27:12.89 Forearm.seek CC @14:27:14.19 CC.seek :closed @14:27:18.74 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.25 @14:27:18.75 Hand.open @14:27:19.44 Forearm.reconfigure :loose @14:27:19.48 Forearm.seek :retract @14:27:20.41 collectSample 100ml w/bubble point of 35psi @14:27:20.43 Priming 7ml @14:27:20.44 Intake.select :close @14:27:20.45 Exhaust.select :close @14:27:20.46 SS.hold @14:27:20.65 Calibrating pressure sensors @14:27:20.66 CTV.dial :air @14:27:20.68 SSV.dial :spit,avoiding: :exhaust @14:27:20.69 SS.reconfigure :fast @14:27:20.74 SS.seek 5ml @14:27:23.48 SSV.dial CBV @14:27:23.50 CBV.dial SSV @14:27:24.89 delay 3.8 seconds @14:27:28.80 SSV.dial :spit @14:27:30.17 SS.seek 0ml @14:27:33.04 SSV.dial CBV @14:27:34.40 CTV.dial :intake @14:27:35.18 SSV.dial CBV @14:27:35.20 CBV.dial SSV @14:27:35.22 Allowing 40s for 7.0ml of remaining 7.0ml @14:27:35.23 Intake.select :open @14:27:37.39 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:27:37.41 SS.reconfigure :inhaling 2.20ml/s @14:27:37.45 SS.seek 7ml @14:27:44.41 Stroke completed -- waiting for pressure to equalize ... @14:27:44.43 SS.reconfigure :exhale @14:27:44.47 SS.hold @14:28:10.97 Intake.select :close @14:28:11.12 SSV.dial :exhaust @14:28:11.87 Exhaust.select :open @14:28:11.98 SS.seek 0ml @14:28:15.94 Exhaust.select :close @14:28:16.05 Sampling 100.0ml @14:28:16.06 Intake.select :close @14:28:16.07 Exhaust.select :close @14:28:16.08 SS.reconfigure :default @14:28:16.12 SS.hold @14:28:16.32 CTV.dial :intake @14:28:16.33 SSV.dial CBV @14:28:16.37 CBV.dial SSV @14:28:17.11 Allowing 130s for 25.0ml of remaining 100.0ml @14:28:17.12 Intake.select :open @14:28:19.27 Intake @14.9psia, vacuum from 9.4 to 12.4psi @14:28:19.29 SS.reconfigure :inhaling 2.20ml/s @14:28:19.34 SS.seek 25ml @14:28:26.38 Vacuum too high -- waiting up to 40 seconds @14:28:26.39 SS.reconfigure :exhale @14:28:26.43 SS.hold @14:28:30.89 Intake @13.5psia, vacuum from 8.0 to 11.0psi @14:28:30.91 SS.reconfigure :inhaling 1.98ml/s @14:28:30.95 SS.seek 25ml @14:28:37.07 Vacuum too high -- waiting up to 40 seconds @14:28:37.08 SS.reconfigure :exhale @14:28:37.12 SS.hold @14:28:44.10 Intake @14.1psia, vacuum from 8.6 to 11.6psi @14:28:44.12 SS.reconfigure :inhaling 1.79ml/s @14:28:44.17 SS.seek 25ml @14:28:49.12 Stroke completed -- waiting for pressure to equalize ... @14:28:49.13 SS.reconfigure :exhale @14:28:49.17 SS.hold @14:28:55.57 Intake.select :close @14:28:55.72 SSV.dial :exhaust @14:28:56.48 Exhaust.select :open @14:28:56.59 SS.seek 0ml @14:29:05.38 Exhaust.select :close @14:29:05.49 SSV.dial CBV @14:29:05.51 CBV.dial SSV @14:29:06.27 Allowing 130s for 25.0ml of remaining 75.0ml @14:29:06.29 Intake.select :open @14:29:08.44 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:29:08.46 SS.reconfigure :inhaling 1.79ml/s @14:29:08.50 SS.seek 25ml @14:29:19.28 Vacuum too high -- waiting up to 40 seconds @14:29:19.29 SS.reconfigure :exhale @14:29:19.34 SS.hold @14:29:24.43 Intake @13.9psia, vacuum from 8.4 to 11.4psi @14:29:24.45 SS.reconfigure :inhaling 1.61ml/s @14:29:24.49 SS.seek 25ml @14:29:32.65 Stroke completed -- waiting for pressure to equalize ... @14:29:32.66 SS.reconfigure :exhale @14:29:32.70 SS.hold @14:29:42.45 Intake.select :close @14:29:42.59 SSV.dial :exhaust @14:29:43.36 Exhaust.select :open @14:29:43.47 SS.seek 0ml @14:29:52.60 Exhaust.select :close @14:29:52.71 SSV.dial CBV @14:29:52.73 CBV.dial SSV @14:29:53.52 Allowing 130s for 25.0ml of remaining 50.0ml @14:29:53.54 Intake.select :open @14:29:55.69 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:29:55.71 SS.reconfigure :inhaling 1.61ml/s @14:29:55.75 SS.seek 25ml @14:30:08.93 Vacuum too high -- waiting up to 40 seconds @14:30:08.95 SS.reconfigure :exhale @14:30:08.99 SS.hold @14:30:13.98 Intake @13.7psia, vacuum from 8.2 to 11.2psi @14:30:13.99 SS.reconfigure :inhaling 1.45ml/s @14:30:14.04 SS.seek 25ml @14:30:20.54 Stroke completed -- waiting for pressure to equalize ... @14:30:20.55 SS.reconfigure :exhale @14:30:21.09 SS.hold @14:30:28.57 Intake.select :close @14:30:28.72 SSV.dial :exhaust @14:30:29.49 Exhaust.select :open @14:30:29.60 SS.seek 0ml @14:30:38.69 Exhaust.select :close @14:30:38.80 SSV.dial CBV @14:30:38.82 CBV.dial SSV @14:30:39.60 Allowing 130s for 25.0ml of remaining 25.0ml @14:30:39.61 Intake.select :open @14:30:41.77 Intake @15.0psia, vacuum from 9.5 to 12.5psi @14:30:41.79 SS.reconfigure :inhaling 1.45ml/s @14:30:41.83 SS.seek 25ml @14:30:59.34 Vacuum too high -- waiting up to 40 seconds @14:30:59.35 SS.reconfigure :exhale @14:30:59.40 SS.hold @14:31:06.01 Intake @13.4psia, vacuum from 7.9 to 10.9psi @14:31:06.03 SS.reconfigure :inhaling 1.30ml/s @14:31:06.07 SS.seek 25ml @14:31:09.30 Stroke completed -- waiting for pressure to equalize ... @14:31:09.31 SS.reconfigure :exhale @14:31:09.36 SS.hold @14:31:11.94 recoverPRVreagent08 @14:31:11.95 PTV.dial :mixing @14:31:12.73 PBV.dial :waste1 @14:31:13.09 delay 30 seconds @14:31:15.00 Intake.select :close @14:31:15.14 SSV.dial :exhaust @14:31:15.93 Exhaust.select :open @14:31:16.04 SS.seek 0ml @14:31:24.82 Exhaust.select :close @14:31:24.92 Sampled 100.0ml @14:31:24.94 Drying puck before fixing for 20:00 @14:31:24.95 CBV.dial :waste2b @14:31:25.69 delay 1:00 @14:31:43.11 PBV.dial between PSV and waste1 @14:31:43.48 PSV.dial :air @14:31:43.97 PS.reconfigure :fast @14:31:44.01 PS.seek 2.5ml @14:32:08.73 PSV.dial :mixloop @14:32:09.20 delay 2 seconds @14:32:11.21 PSV.dial :air @14:32:11.68 delay 2 seconds @14:32:13.70 PSV.dial :mixloop @14:32:14.16 delay 2 seconds @14:32:16.18 PSV.dial :air @14:32:16.65 PS.seek 0ml @14:32:25.71 CSV.dial :air @14:32:26.18 CS.reconfigure :fast @14:32:26.22 CS.seek 10ml @14:32:41.16 washCycleDA #1 of 2 @14:32:41.17 PSR.select :air @14:32:41.19 PSV.dial PSR @14:32:42.59 Pulling 0.5ml of PSR.wash @14:32:42.60 PSR.select :wash @14:32:42.71 PS.reconfigure :default @14:32:42.75 PS.seek 0.5ml @14:32:48.73 delay 5 seconds @14:32:51.07 Pumping wcr puck from tube 2 up to 35psi... @14:32:51.08 CBV.dial SSV @14:32:52.40 CBV.dial between CSV and waste2b @14:32:53.36 CSV.dial between air and CTV @14:32:53.89 PSR.select :air @14:32:54.00 PS.seek 2ml @14:32:54.18 CS.seek 7.319ml @14:33:01.29 CTV.dial CSV,avoiding: :air @14:33:01.31 CSV.dial CTV @14:33:02.13 CS.reconfigure :pressurize @14:33:02.18 CS.seek 0ml @14:33:10.16 PTV.dial :puck @14:33:10.91 PSV.dial PTV @14:33:11.66 PBV.dial :waste1 @14:33:12.10 PS.seek 1.5ml @14:33:15.96 CBV.dial :waste2b @14:33:16.29 CS.reconfigure :default @14:33:16.33 CS.seek 0ml @14:33:18.10 delay 3 seconds @14:33:21.12 PBV.dial between PSV and waste1 @14:33:21.50 PS.seek 0ml @14:33:22.66 delay 1:00 @14:33:37.85 delay 2:00 @14:34:22.68 CTV.dial :air,avoiding: :intake @14:34:24.04 Fix #1 of 2 (with RNAlater) for 10:00 @14:34:24.05 CSV.dial CSR @14:34:24.66 Pulling 2ml of CSR.RNAlater @14:34:24.67 CSR.select :RNAlater @14:34:24.79 CS.seek 2ml @14:34:30.75 delay 5 seconds @14:34:35.77 CSR.select :air @14:34:35.89 CS.reconfigure :fast @14:34:35.93 CS.seek 10ml @14:34:55.97 Pumping wcr puck from tube 2 up to 35psi... @14:34:55.98 CBV.dial SSV @14:34:57.28 CBV.dial between CSV and waste2b @14:34:58.23 CSV.dial between air and CTV @14:34:58.82 CS.seek 7.286ml @14:35:05.88 CTV.dial CSV,avoiding: :air @14:35:05.90 CSV.dial CTV @14:35:07.33 CS.reconfigure :pressurize @14:35:07.38 CS.seek 0ml @14:35:26.53 CSV.dial :air @14:35:27.00 CS.reconfigure :fast @14:35:27.05 CS.seek 10ml @14:35:37.87 recoverPSRreagent08 @14:35:37.89 PTV.dial :puck @14:35:37.90 PBV.dial :waste1 @14:35:38.29 delay 30 seconds @14:35:51.84 CSV.dial between air and CTV @14:35:52.18 CS.seek 6.891ml @14:36:00.13 CTV.dial CSV,avoiding: :air @14:36:00.15 CSV.dial CTV @14:36:00.46 CS.reconfigure :pressurize @14:36:00.51 CS.seek 0ml @14:36:08.30 PBV.dial between PSV and waste1 @14:36:08.69 PSR.select :air @14:36:08.71 PSV.dial PSR @14:36:09.77 PS.reconfigure :fast @14:36:09.82 PS.seek 2.5ml @14:36:18.72 CSV.dial :air @14:36:19.19 CS.reconfigure :fast @14:36:19.24 CS.seek 10ml @14:36:34.50 PSV.dial PTV @14:36:35.39 delay 5 seconds @14:36:40.41 PSV.dial PSR @14:36:41.16 delay 5 seconds @14:36:44.21 CSV.dial between air and CTV @14:36:44.55 CS.seek 7.013ml @14:36:46.18 PSV.dial PTV @14:36:47.07 delay 5 seconds @14:36:52.08 PSV.dial :air @14:36:52.47 CTV.dial CSV,avoiding: :air @14:36:52.50 CSV.dial CTV @14:36:52.80 CS.reconfigure :pressurize @14:36:53.33 PS.seek 0ml @14:36:53.35 CS.seek 0ml @14:37:11.90 CSV.dial :air @14:37:12.49 CS.reconfigure :fast @14:37:12.53 CS.seek 10ml @14:37:17.54 washCycleDA #2 of 2 @14:37:17.55 PSR.select :air @14:37:17.57 PSV.dial PSR @14:37:18.96 Pulling 0.5ml of PSR.wash @14:37:18.97 PSR.select :wash @14:37:19.08 PS.reconfigure :default @14:37:19.13 PS.seek 0.5ml @14:37:25.04 delay 5 seconds @14:37:30.05 PSR.select :air @14:37:30.17 PS.seek 2ml @14:37:37.43 CSV.dial between air and CTV @14:37:37.74 CS.seek 7.155ml @14:37:45.19 CTV.dial CSV,avoiding: :air @14:37:45.21 CSV.dial CTV @14:37:45.52 CS.reconfigure :pressurize @14:37:45.57 CS.seek 0ml @14:37:46.31 PTV.dial :puck @14:37:46.33 PSV.dial PTV @14:37:47.19 PBV.dial :waste1 @14:37:47.62 PS.seek 1.5ml @14:37:53.56 delay 3 seconds @14:37:56.58 PBV.dial between PSV and waste1 @14:37:56.96 PS.seek 0ml @14:38:04.43 CSV.dial :air @14:38:04.89 CS.reconfigure :default @14:38:04.93 CS.hold @14:38:05.00 ShallowSampler::Error in MAIN -- Puck.from 2, :wcr did not reach 35psi after pumping in 40ml of gas @14:38:05.01 Waiting to Resume from Checkpoint "Pumping wcr puck from tube 2 up to 35psi..." @14:38:05.02 BadNews.email "Puck.from 2, :wcr did not reach 35psi after pumping in 40ml of gas",Subject: "ShallowSampler::Error in MAIN" @14:38:13.16 delay 2:00 @14:40:13.20 recoverPSRreagent08 @14:40:13.22 PTV.dial :puck @14:40:13.24 PBV.dial :waste1 @14:40:13.70 delay 30 seconds @14:40:17.35 upsync @14:40:43.76 PBV.dial between PSV and waste1 @14:40:44.14 PSR.select :air @14:40:44.16 PSV.dial PSR @14:40:44.91 PS.reconfigure :fast @14:40:44.96 PS.seek 2.5ml @14:41:09.53 PSV.dial PTV @14:41:10.42 delay 5 seconds @14:41:15.44 PSV.dial PSR @14:41:16.19 delay 5 seconds @14:41:21.21 PSV.dial PTV @14:41:21.97 delay 5 seconds @14:41:26.99 PSV.dial :air @14:41:27.88 PS.seek 0ml @14:41:52.03 getPMLReagent08 @14:41:52.04 PRV.dial :air @14:41:52.48 delay 5 seconds @14:41:57.50 PTV.dial :PRVmixing @14:41:58.84 delay 5 seconds @14:42:03.86 PRV.dial :ab2 @14:42:04.32 PTV.dial :PRVmixing @14:42:04.39 PSV.dial :mixloop @14:42:04.83 PS.reconfigure :default @14:42:04.87 PS.seek 0.3ml @14:42:08.71 delay 2 seconds @14:42:10.74 PRV.dial :air @14:42:11.29 PS.seek 0.55ml @14:42:14.84 addPMLReagent08 @14:42:14.85 PSV.dial :air @14:42:15.45 PS.seek 2ml @14:42:31.06 PSV.dial :PML @14:42:31.51 PTV.dial :mixing @14:42:32.28 PBV.dial :waste1 @14:42:32.70 PS.seek 1.85ml @14:42:35.15 PBV.dial between PSV and waste1 @14:42:35.53 PS.seek 0ml @14:42:55.24 delay 2 seconds @14:42:57.26 delay 8:00 @14:42:59.43 Session Begun @14:43:02.48 -> showlog @14:44:37.60 -> showlog -100 @14:50:57.28 recoverPRVreagent08 @14:50:57.30 PTV.dial :mixing @14:50:57.31 PBV.dial :waste1 @14:50:58.20 delay 30 seconds @14:51:28.23 PBV.dial between PSV and waste1 @14:51:28.61 PSV.dial :air @14:51:29.08 PS.reconfigure :fast @14:51:29.15 PS.seek 2.5ml @14:51:53.70 PSV.dial :mixloop @14:51:54.19 delay 2 seconds @14:51:56.20 PSV.dial :air @14:51:56.67 delay 2 seconds @14:51:58.69 PSV.dial :mixloop @14:51:59.17 delay 2 seconds @14:52:01.19 PSV.dial :air @14:52:01.80 PS.seek 0ml @14:52:25.77 washCycleDA #1 of 3 @14:52:25.78 PSR.select :air @14:52:25.80 PSV.dial PSR @14:52:27.21 Pulling 0.5ml of PSR.wash @14:52:27.22 PSR.select :wash @14:52:27.36 PS.reconfigure :default @14:52:27.41 PS.seek 0.5ml @14:52:33.39 delay 5 seconds @14:52:38.41 PSR.select :air @14:52:38.53 PS.seek 2ml @14:52:54.76 PTV.dial :puck @14:52:55.51 PSV.dial PTV @14:52:56.28 PBV.dial :waste1 @14:52:56.71 PS.seek 1.5ml @14:53:02.73 delay 3 seconds @14:53:05.75 PBV.dial between PSV and waste1 @14:53:06.15 PS.seek 0ml @14:53:22.48 delay 2:00 @14:55:22.51 recoverPSRreagent08 @14:55:22.52 PTV.dial :puck @14:55:22.55 PBV.dial :waste1 @14:55:22.93 delay 30 seconds @14:55:52.96 PBV.dial between PSV and waste1 @14:55:53.34 PSR.select :air @14:55:53.36 PSV.dial PSR @14:55:54.14 PS.reconfigure :fast @14:55:54.20 PS.seek 2.5ml @14:56:18.79 PSV.dial PTV @14:56:19.68 delay 5 seconds @14:56:24.70 PSV.dial PSR @14:56:25.46 delay 5 seconds @14:56:30.48 PSV.dial PTV @14:56:31.26 delay 5 seconds @14:56:36.28 PSV.dial :air @14:56:37.05 PS.seek 0ml @14:57:01.07 washCycleDA #2 of 3 @14:57:01.08 PSR.select :air @14:57:01.11 PSV.dial PSR @14:57:02.53 Pulling 0.5ml of PSR.wash @14:57:02.54 PSR.select :wash @14:57:02.68 PS.reconfigure :default @14:57:02.74 PS.seek 0.5ml @14:57:08.70 delay 5 seconds @14:57:13.72 PSR.select :air @14:57:13.85 PS.seek 2ml @14:57:30.04 PTV.dial :puck @14:57:30.05 PSV.dial PTV @14:57:30.82 PBV.dial :waste1 @14:57:31.25 PS.seek 1.5ml @14:57:37.16 delay 3 seconds @14:57:40.18 PBV.dial between PSV and waste1 @14:57:40.56 PS.seek 0ml @14:57:56.94 delay 2:00 @14:59:56.97 recoverPSRreagent08 @14:59:56.98 PTV.dial :puck @14:59:57.01 PBV.dial :waste1 @14:59:57.38 delay 30 seconds @15:00:27.41 PBV.dial between PSV and waste1 @15:00:27.79 PSR.select :air @15:00:27.81 PSV.dial PSR @15:00:28.59 PS.reconfigure :fast @15:00:28.65 PS.seek 2.5ml @15:00:53.16 PSV.dial PTV @15:00:54.07 delay 5 seconds @15:00:59.09 PSV.dial PSR @15:00:59.83 delay 5 seconds @15:01:04.85 PSV.dial PTV @15:01:05.63 delay 5 seconds @15:01:10.65 PSV.dial :air @15:01:11.57 PS.seek 0ml @15:01:35.72 washCycleDA #3 of 3 @15:01:35.73 PSR.select :air @15:01:35.76 PSV.dial PSR @15:01:37.18 Pulling 0.5ml of PSR.wash @15:01:37.19 PSR.select :wash @15:01:37.33 PS.reconfigure :default @15:01:37.39 PS.seek 0.5ml @15:01:43.41 delay 5 seconds @15:01:48.43 PSR.select :air @15:01:48.55 PS.seek 2ml @15:02:04.72 PTV.dial :puck @15:02:04.73 PSV.dial PTV @15:02:05.52 PBV.dial :waste1 @15:02:05.97 PS.seek 1.5ml @15:02:11.91 delay 3 seconds @15:02:14.93 PBV.dial between PSV and waste1 @15:02:15.31 PS.seek 0ml @15:02:31.47 delay 2:00 @15:04:31.50 recoverPSRreagent08 @15:04:31.51 PTV.dial :puck @15:04:31.52 PBV.dial :waste1 @15:04:31.92 delay 30 seconds @15:05:01.94 PBV.dial between PSV and waste1 @15:05:02.33 PSR.select :air @15:05:02.35 PSV.dial PSR @15:05:03.13 PS.reconfigure :fast @15:05:03.19 PS.seek 2.5ml @15:05:28.28 PSV.dial PTV @15:05:29.05 delay 5 seconds @15:05:34.06 PSV.dial PSR @15:05:34.84 delay 5 seconds @15:05:39.86 PSV.dial PTV @15:05:40.64 delay 5 seconds @15:05:45.66 PSV.dial :air @15:05:46.44 PS.seek 0ml @15:06:10.40 Gate.power :camera,:ON @15:06:10.42 Mixing DA Substrates @15:06:10.44 PSR.select :air @15:06:10.46 PSV.dial PSR @15:06:12.06 Pulling 0.25ml of PSR.sub1 @15:06:12.09 PSR.select :sub1 @15:06:12.35 PS.reconfigure :default @15:06:12.47 PS.seek 0.25ml @15:06:15.97 delay 5 seconds @15:06:21.03 Pulling 0.25ml of PSR.sub2 @15:06:21.04 PSR.select :sub2 @15:06:21.18 PS.seek 0.5ml @15:06:24.65 delay 5 seconds @15:06:29.67 PSR.select :air @15:06:29.80 PS.seek 2ml @15:06:45.93 PTV.dial :puck @15:06:45.94 PSV.dial PTV @15:06:46.75 PBV.dial :waste1 @15:06:47.21 PS.seek 1.5ml @15:06:53.09 delay 3 seconds @15:06:56.11 PBV.dial between PSV and waste1 @15:06:56.52 PS.seek 0ml @15:07:12.87 delay 10 seconds @15:07:22.90 recoverPSRreagent08 @15:07:22.91 PTV.dial :puck @15:07:22.94 PBV.dial :waste1 @15:07:23.34 delay 30 seconds @15:07:53.36 PBV.dial between PSV and waste1 @15:07:53.76 PSR.select :air @15:07:53.78 PSV.dial PSR @15:07:54.59 PS.reconfigure :fast @15:07:54.66 PS.seek 2.5ml @15:08:20.18 PSV.dial PTV @15:08:20.96 delay 5 seconds @15:08:25.98 PSV.dial PSR @15:08:26.79 delay 5 seconds @15:08:31.81 PSV.dial PTV @15:08:32.64 delay 5 seconds @15:08:37.66 PSV.dial :air @15:08:38.47 PS.seek 0ml @15:09:04.06 Additional air clear @15:09:04.07 PSR.select :air @15:09:04.10 PSV.dial PSR @15:09:05.56 Pulling 2ml of PSR.air @15:09:05.57 PSR.select :air @15:09:05.61 PS.reconfigure :default @15:09:05.68 PS.seek 2ml @15:09:26.90 delay 5 seconds @15:09:31.91 PTV.dial :puck @15:09:31.93 PSV.dial PTV @15:09:32.73 PBV.dial :waste1 @15:09:33.16 PS.seek 1.5ml @15:09:39.12 delay 3 seconds @15:09:42.13 PBV.dial between PSV and waste1 @15:09:42.54 delay 1 second @15:09:43.56 PSV.dial :waste1 @15:09:44.71 PS.seek 0ml @15:10:00.92 Processing Puck Heater at 28.2C before imaging @15:10:00.93 PH.coast @15:10:01.07 Imaging da puck in Processing Clamp while storing it to tube 1 @15:10:01.24 SC.reconfigure :tight @15:10:01.30 SC.seek 1 @15:10:04.93 SC.configure :limp @15:10:05.14 Elbow.reconfigure :tight @15:10:05.20 Elbow.seek 1 @15:10:06.97 Forearm.reconfigure :tight @15:10:07.03 Forearm.seek 1 + 450 counts @15:10:08.07 SE.reconfigure :pick @15:10:08.14 SE.seek :beamBreak @15:10:11.23 SE.configure :default @15:10:11.39 Elbow.reconfigure :limp @15:10:11.45 Forearm.reconfigure :loose @15:10:11.51 Forearm.seek 1 @15:10:11.73 Elbow.hold @15:10:11.81 Elbow.reconfigure :tight @15:10:11.92 SE.seek 1.17 Pucks below Top Plate @15:10:12.38 Forearm.force -20 @15:10:12.43 Forearm.seek 1 + 302 counts @15:10:12.84 Forearm.reconfigure :tight @15:10:12.91 Forearm.seek 1 + 450 counts @15:10:13.21 SE.seek 2.17 Pucks below Top Plate @15:10:13.73 Forearm.reconfigure :loose @15:10:13.79 Forearm.seek :retract @15:10:14.75 Elbow.seek PC @15:10:17.47 Elbow.reconfigure :loose @15:10:17.58 Forearm.seek PC @15:10:18.53 Hand.close @15:10:19.84 PC.seek :open @15:10:24.62 Forearm.seek :retract @15:10:25.59 Elbow.reconfigure :tight @15:10:25.66 Elbow.seek 1 @15:10:28.33 Forearm.reconfigure :tight @15:10:28.39 Forearm.seek 1 + 450 counts @15:10:29.42 Hand.open @15:10:30.28 SE.seek 1.82 Pucks below Top Plate @15:10:30.56 SE.reconfigure :seal @15:10:30.62 SE.seek 0.86 Pucks below Top Plate @15:10:31.18 Lights, camera, ACTION! @15:10:31.23 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21nov0815h100ml.tif @15:10:50.70 Waiting >20 seconds for da to relinquish FlushPuck @15:11:11.91 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 37.172 seconds /var/log/esp/da21nov0815h100ml.tif: TIFF Upload Complete @15:11:11.94 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21nov0815h100ml15s.tif @15:11:27.59 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21nov0815h100ml15s.tif: TIFF Upload Complete @15:11:27.62 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21nov0815h100ml30s.tif @15:11:58.31 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21nov0815h100ml30s.tif: TIFF Upload Complete @15:11:58.34 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21nov0815h100ml70s.tif @15:13:10.21 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21nov0815h100ml70s.tif: TIFF Upload Complete @15:13:10.22 Gate.power :camera,:OFF @15:13:10.25 DAfans.email "37.172 sec auto-exposure",Subject: "da21nov0815h100ml images!" @15:13:10.54 Elbow.seek 1 @15:13:11.03 Forearm.seek 1 + 450 counts @15:13:11.14 SE.reconfigure :gently @15:13:11.20 SE.seek 1.57 Pucks below Top Plate @15:13:11.85 Elbow.reconfigure :limp @15:13:11.93 Forearm.reconfigure :loose @15:13:11.99 Forearm.seek 1 @15:13:12.26 Elbow.hold @15:13:12.36 Elbow.reconfigure :tight @15:13:12.43 SE.reconfigure :default @15:13:12.49 SE.seek 1.76 Pucks below Top Plate @15:13:12.92 Forearm.force -20 @15:13:12.99 Forearm.seek 1 + 302 counts @15:13:13.44 Forearm.reconfigure :tight @15:13:13.51 Forearm.seek 1 + 450 counts @15:13:13.77 SE.seek :down @15:13:14.90 Forearm.reconfigure :loose @15:13:14.96 Forearm.seek :retract @15:13:16.36 SE.stop @15:13:16.48 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:13:16.68 Elbow.seek FlushPuck::Garage @15:13:18.62 Elbow.reconfigure :loose @15:13:18.84 Forearm.seek :garage @15:13:19.93 Hand.close @15:13:20.84 Forearm.seek :retract @15:13:21.95 Elbow.reconfigure :tight @15:13:22.06 Elbow.seek PC @15:13:23.46 Elbow.reconfigure :loose @15:13:23.74 Forearm.reconfigure :tight @15:13:23.87 Forearm.seek PC @15:13:25.37 PC.seek :closed @15:13:30.17 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.66 @15:13:30.36 Hand.open @15:13:32.27 Forearm.reconfigure :loose @15:13:32.40 Forearm.seek :retract @15:13:33.47 PSR.select :air @15:13:33.52 PSV.dial PSR @15:13:34.18 Pulling 0.5ml of PSR.flush @15:13:34.20 PSR.select :flush @15:13:34.43 PS.seek 0.5ml @15:13:40.45 delay 5 seconds @15:13:45.47 PSR.select :air @15:13:45.67 PS.seek 2.5ml @15:14:06.94 delay 1 second @15:14:07.98 PRV.dial :waste2,avoiding: :ab2 @15:14:09.94 PTV.dial :PRVmixing @15:14:11.34 PSV.dial :mixloop @15:14:12.53 PS.seek :empty @15:14:38.97 PSR.select :air @15:14:39.01 PSV.dial PSR @15:14:40.29 Pulling 0.5ml of PSR.flush @15:14:40.31 PSR.select :flush @15:14:40.53 PS.seek 0.5ml @15:14:46.52 delay 5 seconds @15:14:51.56 PSR.select :air @15:14:51.76 PS.seek 2.5ml @15:15:13.17 delay 1 second @15:15:14.21 PBV.dial :waste1 @15:15:14.64 PTV.dial :mixing @15:15:15.47 PSV.dial :mixloop @15:15:16.59 PS.seek :empty @15:15:43.10 PSV.dial PSR @15:15:44.27 PSR.select :flush @15:15:44.47 PS.seek 1ml @15:15:54.70 upsync @15:15:55.70 PSR.select :air @15:15:55.83 PS.seek 2.5ml @15:16:11.98 PSV.dial PTV @15:16:12.77 PTV.dial :puck @15:16:13.53 PBV.dial :waste1 @15:16:13.55 PS.seek 0ml @15:16:39.95 PSV.dial PSR @15:16:40.72 PSR.select :flush @15:16:40.85 PS.seek 0.5ml @15:16:46.82 PSR.select :air @15:16:46.96 PS.seek 2.5ml @15:17:08.20 PSV.dial PTV @15:17:09.09 PBV.dial PSV @15:17:09.69 PS.seek 0ml @15:17:36.23 PSV.dial PBV @15:17:36.71 PS.seek 2.5ml @15:18:03.14 PSV.dial :waste1 @15:18:03.91 PS.seek 0ml @15:18:30.29 PSV.dial PSR @15:18:30.78 PSR.select :air @15:18:30.81 PS.seek 2.5ml @15:18:57.14 PSV.dial PTV @15:18:57.90 PBV.dial PSV @15:18:57.92 PS.seek 0ml @15:19:24.38 PSV.dial PBV @15:19:24.99 PS.seek 2.5ml @15:19:51.42 PSV.dial :waste1 @15:19:52.20 PS.seek 0ml @15:20:18.60 PSV.dial PSR @15:20:19.09 PSR.select :flush @15:20:19.22 PS.seek 1ml @15:20:30.23 PSR.select :air @15:20:30.36 PS.seek 2.5ml @15:20:46.57 PSV.dial PTV @15:20:47.34 PTV.dial :puck @15:20:47.35 PBV.dial :waste1 @15:20:47.95 PS.seek 0ml @15:21:14.26 PSV.dial PSR @15:21:15.04 PSR.select :flush @15:21:15.17 PS.seek 0.5ml @15:21:21.07 PSR.select :air @15:21:21.20 PS.seek 2.5ml @15:21:42.66 PSV.dial PTV @15:21:43.45 PBV.dial PSV @15:21:44.05 PS.seek 0ml @15:22:10.41 PSV.dial PBV @15:22:10.90 PS.seek 2.5ml @15:22:37.29 PSV.dial :waste1 @15:22:38.06 PS.seek 0ml @15:23:04.41 PSV.dial PSR @15:23:04.88 PSR.select :air @15:23:04.91 PS.seek 2.5ml @15:23:31.25 PSV.dial PTV @15:23:32.01 PBV.dial PSV @15:23:32.03 PS.seek 0ml @15:23:58.60 PSV.dial PBV @15:23:59.54 PS.seek 2.5ml @15:24:25.94 PSV.dial :waste1 @15:24:26.71 PS.seek 0ml @15:24:53.07 Garaging FlushPuck in Processing Clamp @15:24:53.16 Elbow.reconfigure :tight @15:24:53.22 Elbow.seek PC @15:24:53.35 Elbow.reconfigure :loose @15:24:53.46 Forearm.seek PC @15:24:54.42 Hand.close @15:24:55.08 PC.seek :open @15:24:59.80 Forearm.seek :retract @15:25:00.85 Elbow.reconfigure :tight @15:25:00.91 Elbow.seek :garage @15:25:02.26 Elbow.reconfigure :loose @15:25:02.37 Forearm.reconfigure :tight @15:25:02.43 Forearm.seek :garage @15:25:03.48 Hand.open @15:25:04.14 Forearm.reconfigure :loose @15:25:04.20 Forearm.seek :retract @15:32:37.87 Session Ended @13:15:05.64UTC09-Nov-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:15:08.17 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:15:11.77 Polling Can every 10:00 @13:15:11.81 Cleanly exited at 13:09:16UTC09-Nov-21 @13:15:11.84 Loading /home/esp/esp2/mission/DA_WCR_110921.rb mission @13:15:12.22 GoodNews.email "Starting DA_WCR_110921 w/pucks in tube 2\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:15:11, 16% flow, 22.0C, 13% humidity, 15.2psia, 13.535V, 0.375A, 0.357A avg, 5.07W",Subject: "DA_WCR_110921 begun" @13:15:12.25
Preparing to sleep until + 00:00:05 @13:15:12.29 Asleep til + 00:00:05 @13:15:12.31 Continuing at 13:15 @13:15:12.59 Gate.power :raw,:OFF @13:15:12.61 Gate.power :core,:OFF @13:15:12.62 Shutdown @13:15:16.14 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110921.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:15:12, 16% flow, 22.0C, 13% humidity, 15.2psia, 13.535V, 0.375A, 0.357A avg, 5.07W",Subject: "Asleep until 13:15" @13:15:16.87
Powering core bus @13:15:22.28 Gate.power :raw,:ON @13:15:23.56 CS.configure :default @13:15:23.75 PS.configure :default @13:15:23.95 SS.configure :default @13:15:24.13 CC.configure :default @13:15:24.32 PC.configure :default @13:15:24.53 Forearm.configure :loose @13:15:24.78 Elbow.configure :loose @13:15:24.99 SC.configure :loose @13:15:25.16 SE.configure :default @13:15:25.26 CH.coast @13:15:25.37 CH.configure :default @13:15:25.43 PH.coast @13:15:25.54 PH.configure :default @13:15:25.62 All dwarf controllers running firmware version 3.85 @13:15:25.75 Valve.reset @13:15:26.27 Intake.select :close @13:15:26.35 Exhaust.select :close @13:15:26.37 CTV.dial :air @13:15:26.54 PSV.dial :air @13:15:26.78
Forearm.reconfigure :loose @13:15:26.86 CSV.dial :waste1 @13:15:26.93 PS.reconfigure :default @13:15:27.03
Forearm.home.seek :retract @13:15:27.23 PS.home.seek 0ml @13:15:27.33 SSV.dial :spit,avoiding: :exhaust @13:15:27.55 CS.reconfigure :fast @13:15:27.62 SS.reconfigure :default @13:15:27.83 CS.home.seek 0ml @13:15:27.89 SS.home.seek 0ml @13:15:28.06 Hand.close @13:15:28.12
Elbow.jog 1500 @13:15:28.81 Elbow.reconfigure :loose @13:15:28.89 Elbow.home.seek :clear @13:15:33.42 SE.reconfigure :default @13:15:33.50 SE.home.seek :down @13:15:34.19 SC.reconfigure :tight @13:15:34.28 SC.home.seek :initial @13:15:35.55 PS.stop @13:15:46.97
SC.configure :limp @13:15:47.07 Forearm.seek :retract @13:15:47.20 Elbow.seek :hammer @13:15:48.33 Forearm.reconfigure :tight @13:15:48.41 Forearm.seek retracted @13:15:48.96 PC.reconfigure :default @13:15:48.98
CC.reconfigure :default @13:15:49.17 PC.home.seek :open @13:15:49.22
CC.home.seek :open @13:15:53.33 Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @13:15:53.36 Notice.email "At 13:15:53 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_110921.rb:14\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:15:53, 16% flow, 21.9C, 12% humidity, 15.2psia, 13.574V, 0.315A, 0.296A avg, 4.27W",Subject: "Started DA_WCR_110921 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @13:15:53.42
Starting initialPurge @13:15:53.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:15:53.68 Hand.open @13:15:54.65 Elbow.reconfigure :tight @13:15:54.74 Elbow.seek FlushPuck::Garage @13:15:56.68 Elbow.reconfigure :loose @13:15:56.87 Forearm.reconfigure :loose @13:15:56.95 Forearm.seek :garage @13:15:58.09 Hand.close @13:15:58.92 Forearm.seek :retract @13:16:00.15 Elbow.reconfigure :tight @13:16:00.23 Elbow.seek CC @13:16:03.27 Elbow.reconfigure :loose @13:16:03.45 Forearm.reconfigure :tight @13:16:03.57 Forearm.seek CC @13:16:05.29 CC.seek :closed @13:16:10.01 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.27 @13:16:10.15 Hand.open @13:16:10.95 Forearm.reconfigure :loose @13:16:11.03 Forearm.seek :retract @13:16:12.02 Flushing the Collection Valve::Manifold @13:16:12.26 CSV.dial CSR @13:16:13.74 Pulling 5ml of CSR.flush @13:16:13.76 CSR.select :flush @13:16:13.92 CS.reconfigure :default @13:16:14.01 CS.seek 5ml @13:16:27.71 delay 5 seconds @13:16:32.74 CSR.select :air @13:16:33.00 CS.reconfigure :fast @13:16:33.09 CS.seek 7.499ml @13:16:39.96 CSV.dial :waste @13:16:41.34 CS.reconfigure :default @13:16:41.43 CS.seek 0ml @13:17:01.50 Purging external Intake @13:17:01.73 CSV.dial :flush @13:17:02.22 CS.reconfigure :fast @13:17:02.30 CS.seek 10ml @13:17:27.14 delay 5 seconds @13:17:32.16 CSV.dial CBV @13:17:32.20
CBV.dial CSV @13:17:33.05 CTV.dial :intake @13:17:33.87 Intake.select :open @13:17:34.03 CS.seek 0ml @13:17:36.16 upsync @13:17:57.97
Intake.select :close @13:17:58.07 CTV.dial :air @13:17:58.87 Flushing upper manifold @13:17:58.98 CSV.dial :flush @13:17:59.72 CS.seek 5ml @13:18:12.54 delay 5 seconds @13:18:17.56 CSV.dial :air @13:18:18.07 CS.seek 7.001ml @13:18:23.66 CSV.dial CBV @13:18:23.69
CBV.dial CSV @13:18:24.71 CTV.dial :air @13:18:24.73 CS.seek 0ml @13:18:41.72 delay 7 seconds @13:18:48.75 CS.seek 3ml @13:18:56.88 CSV.dial :waste @13:18:57.32 CS.seek 0ml @13:19:05.29 CSV.dial :flush @13:19:05.75 CS.seek 3ml @13:19:13.91 delay 5 seconds @13:19:18.93 CSV.dial :air @13:19:19.43 CS.seek 5ml @13:19:25.28 CSV.dial :air @13:19:25.30 CS.seek 0ml @13:19:37.66 delay 7 seconds @13:19:44.69 CS.seek 3ml @13:19:53.00 CSV.dial :waste @13:19:53.73 CS.seek 0ml @13:20:01.57 Garaging FlushPuck in Collection Clamp @13:20:01.64 Elbow.reconfigure :tight @13:20:01.68 Elbow.seek CC @13:20:01.99 Elbow.reconfigure :loose @13:20:02.07 Forearm.seek CC @13:20:03.10 Hand.close @13:20:03.68 CC.seek :open @13:20:08.30 Forearm.seek :retract @13:20:09.28 Elbow.reconfigure :tight @13:20:09.32 Elbow.seek :garage @13:20:12.25 Elbow.reconfigure :loose @13:20:12.34 Forearm.reconfigure :tight @13:20:12.38 Forearm.seek :garage @13:20:13.36 Hand.open @13:20:13.99 Forearm.reconfigure :loose @13:20:14.44 Forearm.seek :retract @13:20:15.47 da sampling at most 100ml, wcr at most 100ml @13:20:15.50 Loading sh1 puck from tube 2 into Collection Clamp @13:20:15.51 DAfans.email "Started da in tube 2 at 13:20:15UTC09-Nov-21\n{1 => 4,\n 2 => 29,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:15:53, 16% flow, 21.9C, 12% humidity, 15.2psia, 13.574V, 0.315A, 0.296A avg, 4.27W",Subject: "da sampling at most 100ml, wcr at most 100ml" @13:20:15.88
SC.reconfigure :tight @13:20:15.92 SC.seek 2 @13:20:22.75 SC.configure :limp @13:20:23.21 Elbow.reconfigure :tight @13:20:23.34 Elbow.seek 2 @13:20:25.40 Forearm.reconfigure :tight @13:20:25.56 Forearm.seek 2 + 450 counts @13:20:26.67 SE.reconfigure :pick @13:20:26.77 SE.seek :beamBreak @13:20:28.19 SE.configure :default @13:20:28.49 Elbow.seek 2 @13:20:28.93 Forearm.seek 2 + 300 counts @13:20:29.25 Forearm.reconfigure :loose @13:20:29.33 Hand.close @13:20:29.99 SE.seek :down @13:20:30.37
Forearm.seek :retract @13:20:30.90 SE.stop @13:20:31.43
Elbow.seek CC @13:20:33.22 Elbow.reconfigure :loose @13:20:33.42 Forearm.reconfigure :tight @13:20:33.50 Forearm.seek CC @13:20:35.11 CC.seek :closed @13:20:39.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.33 @13:20:39.76 Hand.open @13:20:40.66 Forearm.reconfigure :loose @13:20:40.73 Forearm.seek :retract @13:20:41.75 collectSample 100ml w/bubble point of 24psi @13:20:41.77 Priming 7ml @13:20:41.79 Intake.select :close @13:20:41.81 Exhaust.select :close @13:20:41.83 SS.hold @13:20:42.21 Calibrating pressure and temperature sensors @13:20:42.27 CTV.dial :air @13:20:42.29 SSV.dial :spit,avoiding: :exhaust @13:20:42.33 SS.reconfigure :fast @13:20:42.41 SS.seek 5ml @13:20:45.44 SSV.dial CBV @13:20:45.48
CBV.dial SSV @13:20:46.91 delay 3.8 seconds @13:20:50.91 SSV.dial :spit @13:20:52.31 SS.seek 0ml @13:20:55.37 SSV.dial CBV @13:20:56.73 CTV.dial :intake @13:20:57.56 SSV.dial CBV @13:20:57.61
CBV.dial SSV @13:20:57.72 Allowing 40s for 7.0ml of remaining 7.0ml @13:20:57.74 Intake.select :open @13:21:00.00 Intake @14.2psia, vacuum from 8.7 to 11.7psi @13:21:00.03 SS.reconfigure :inhaling 2.20ml/s @13:21:00.17 SS.seek 7ml @13:21:06.75 Stroke completed -- waiting for pressure to equalize ... @13:21:06.77 SS.reconfigure :exhale @13:21:06.85 SS.hold @13:21:09.77 Intake.select :close @13:21:09.98 SSV.dial :exhaust @13:21:10.82 Exhaust.select :open @13:21:10.97 SS.seek 0ml @13:21:15.01 Exhaust.select :close @13:21:15.15 Sampling 100.0ml @13:21:15.17 Intake.select :close @13:21:15.19 Exhaust.select :close @13:21:15.21 SS.reconfigure :default @13:21:15.28 SS.hold @13:21:15.66 CTV.dial :intake @13:21:15.69 SSV.dial CBV @13:21:15.74
CBV.dial SSV @13:21:16.58 Allowing 130s for 25.0ml of remaining 100.0ml @13:21:16.60 Intake.select :open @13:21:18.84 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:21:18.87 SS.reconfigure :inhaling 2.20ml/s @13:21:18.95 SS.seek 25ml @13:21:29.67 Vacuum too high -- waiting up to 40 seconds @13:21:29.68 SS.reconfigure :exhale @13:21:29.76 SS.hold @13:21:33.45 Intake @12.5psia, vacuum from 7.0 to 10.0psi @13:21:33.48 SS.reconfigure :inhaling 1.98ml/s @13:21:33.57 SS.seek 25ml @13:21:39.80 Stroke completed -- waiting for pressure to equalize ... @13:21:39.82 SS.reconfigure :exhale @13:21:39.90 SS.hold @13:21:46.09 Intake.select :close @13:21:46.31 SSV.dial :exhaust @13:21:47.10 Exhaust.select :open @13:21:47.25 SS.seek 0ml @13:21:56.13 Exhaust.select :close @13:21:56.28 SSV.dial CBV @13:21:56.31
CBV.dial SSV @13:21:57.14 Allowing 130s for 25.0ml of remaining 75.0ml @13:21:57.16 Intake.select :open @13:21:59.39 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:21:59.41 SS.reconfigure :inhaling 1.98ml/s @13:21:59.49 SS.seek 25ml @13:22:11.80 Vacuum too high -- waiting up to 40 seconds @13:22:11.82 SS.reconfigure :exhale @13:22:11.90 SS.hold @13:22:12.65 Intake @12.0psia, vacuum from 6.5 to 9.5psi @13:22:12.69 SS.reconfigure :inhaling 1.79ml/s @13:22:12.77 SS.seek 25ml @13:22:18.18 Vacuum too high -- waiting up to 40 seconds @13:22:18.20 SS.reconfigure :exhale @13:22:18.29 SS.hold @13:22:25.62 Intake @13.7psia, vacuum from 8.2 to 11.2psi @13:22:25.65 SS.reconfigure :inhaling 1.61ml/s @13:22:25.74 SS.seek 25ml @13:22:26.02 Stroke completed -- waiting for pressure to equalize ... @13:22:26.04 SS.reconfigure :exhale @13:22:26.12 SS.hold @13:22:27.24 Intake.select :close @13:22:27.46 SSV.dial :exhaust @13:22:28.26 Exhaust.select :open @13:22:28.41 SS.seek 0ml @13:22:37.23 Exhaust.select :close @13:22:37.37 SSV.dial CBV @13:22:37.42
CBV.dial SSV @13:22:38.27 Allowing 130s for 25.0ml of remaining 50.0ml @13:22:38.29 Intake.select :open @13:22:40.54 Intake @14.6psia, vacuum from 9.1 to 12.1psi @13:22:40.56 SS.reconfigure :inhaling 1.61ml/s @13:22:40.64 SS.seek 25ml @13:22:43.25 upsync @13:22:59.73
Stroke completed -- waiting for pressure to equalize ... @13:22:59.74 SS.reconfigure :exhale @13:22:59.78 SS.hold @13:23:09.44 Intake.select :close @13:23:09.58 SSV.dial :exhaust @13:23:10.33 Exhaust.select :open @13:23:10.44 SS.seek 0ml @13:23:19.47 Exhaust.select :close @13:23:19.57 SSV.dial CBV @13:23:19.59
CBV.dial SSV @13:23:20.36 Allowing 130s for 25.0ml of remaining 25.0ml @13:23:20.37 Intake.select :open @13:23:22.52 Intake @14.7psia, vacuum from 9.2 to 12.2psi @13:23:22.54 SS.reconfigure :inhaling 1.61ml/s @13:23:22.58 SS.seek 25ml @13:23:40.97 Stroke completed -- waiting for pressure to equalize ... @13:23:40.98 SS.reconfigure :exhale @13:23:41.02 SS.hold @13:23:50.67 Intake.select :close @13:23:50.81 SSV.dial :exhaust @13:23:51.57 Exhaust.select :open @13:23:51.67 SS.seek 0ml @13:24:00.73 Exhaust.select :close @13:24:00.84 Sampled 100.0ml @13:24:00.85 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:24:00.86 Intake.select :close @13:24:00.92 CSV.dial :waste @13:24:00.98 CS.seek 0ml @13:24:01.05 CSV.dial :air @13:24:01.08 SSV.dial CBV,avoiding: :exhaust @13:24:01.12
CBV.dial SSV @13:24:01.96 CS.seek 10ml @13:24:03.03
CTV.dial :air @13:24:03.83 delay 15 seconds @13:24:18.85 SSV.dial :spit,avoiding: :exhaust @13:24:20.65 CTV.dial CSV,avoiding: :intake @13:24:20.70
SS.seek 0ml @13:24:20.80 SSV.dial CBV,avoiding: :exhaust @13:24:26.78 CBV.dial between SSV and waste2 @13:24:27.16 CTV.dial CSV,avoiding: :air @13:24:27.18
CSV.dial CTV @13:24:27.65 CS.reconfigure :pressurize @13:24:27.69 CS.seek 0ml @13:24:46.13 BubblePoint: Intake @39.3psia, Outlet @15.2psia @13:24:46.14 CBV.dial SSV @13:24:46.46 SS.reconfigure :default @13:24:46.50 SS.seek 10ml @13:24:51.98 delay 15 seconds @13:25:07.10 Evacuated: Intake @16.2psia, Outlet @10.6psia @13:25:07.11 SSV.dial :spit,avoiding: :exhaust @13:25:08.44 SS.reconfigure :exhale @13:25:08.48 SS.seek 0ml @13:25:13.88 SS.reconfigure :fast @13:25:13.92 SS.seek 5ml @13:25:16.88 SSV.dial CBV,avoiding: :exhaust @13:25:16.90
CBV.dial SSV @13:25:18.25 CTV.dial :air,avoiding: :intake @13:25:19.61 CSV.dial CTV @13:25:19.65
SS.seek 10ml @13:25:20.64 CSV.dial :air @13:25:21.11 CS.reconfigure :fast @13:25:21.16 CS.seek 0ml @13:25:22.12
SSV.dial :spit,avoiding: :exhaust @13:25:23.47 SS.reconfigure :exhale @13:25:23.52 SS.seek 0ml @13:25:28.56 SS.reconfigure :fast @13:25:28.60 SS.seek 5ml @13:25:29.09 CS.stop @13:25:31.77 SSV.dial CBV,avoiding: :exhaust @13:25:31.80
CBV.dial SSV @13:25:34.15 SSV.dial :spit,avoiding: :exhaust @13:25:35.45 SS.reconfigure :default @13:25:35.50 SS.seek 0ml @13:25:38.57 SS.stop @13:25:38.61 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:25:38.62 Intake.select :close @13:25:38.69 CSV.dial :waste @13:25:38.81 SSV.dial CBV,avoiding: :exhaust @13:25:38.82
CBV.dial SSV @13:25:39.44 CS.seek 0ml @13:25:39.50 CSV.dial :air @13:25:40.17
CTV.dial :air @13:25:40.18 delay 15 seconds @13:25:40.26 CS.seek 10ml @13:25:55.20
SSV.dial :spit,avoiding: :exhaust @13:25:56.52 CTV.dial CSV,avoiding: :intake @13:25:56.55
SS.reconfigure :exhale @13:25:56.64 SS.seek 0ml @13:25:57.03 SSV.dial CBV,avoiding: :exhaust @13:26:05.02 CBV.dial between SSV and waste2 @13:26:05.41 CTV.dial CSV,avoiding: :air @13:26:05.43
CSV.dial CTV @13:26:05.86 CS.reconfigure :pressurize @13:26:05.91 CS.seek 0ml @13:26:27.12 BubblePoint: Intake @38.4psia, Outlet @14.4psia @13:26:27.13 CBV.dial SSV @13:26:27.46 SS.reconfigure :default @13:26:27.50 SS.seek 10ml @13:26:33.17 delay 15 seconds @13:26:48.28 Evacuated: Intake @17.8psia, Outlet @12.1psia @13:26:48.29 SSV.dial :spit,avoiding: :exhaust @13:26:49.63 SS.reconfigure :exhale @13:26:49.67 SS.seek 0ml @13:26:55.04 SS.reconfigure :fast @13:26:55.08 SS.seek 5ml @13:26:58.25 SSV.dial CBV,avoiding: :exhaust @13:26:58.27
CBV.dial SSV @13:26:59.61 CTV.dial :air,avoiding: :intake @13:27:00.97 CSV.dial CTV @13:27:01.00
SS.seek 10ml @13:27:01.99 CSV.dial :air @13:27:02.44 CS.reconfigure :fast @13:27:02.48 CS.seek 0ml @13:27:03.65
SSV.dial :spit,avoiding: :exhaust @13:27:04.99 SS.reconfigure :exhale @13:27:05.03 SS.seek 0ml @13:27:07.75 CS.stop @13:27:10.11
SS.reconfigure :fast @13:27:10.16 SS.seek 5ml @13:27:13.36 SSV.dial CBV,avoiding: :exhaust @13:27:13.38
CBV.dial SSV @13:27:15.72 SSV.dial :spit,avoiding: :exhaust @13:27:17.05 SS.reconfigure :default @13:27:17.09 SS.seek 0ml @13:27:20.13 SS.stop @13:27:20.17 Lysing: Adding 2.0ml AqMeOH @13:27:20.18 CBV.dial between CSV and waste1,avoiding: :DBV @13:27:21.66 CTV.dial CSV @13:27:22.98 CSV.dial CSR @13:27:23.75 Pulling 0.999ml of CSR.AqMeOH @13:27:23.76 CSR.select :AqMeOH @13:27:23.87 CS.reconfigure :default @13:27:23.91 CS.seek 1ml @13:27:27.25 delay 5 seconds @13:27:32.27 CSR.select :air @13:27:32.42 CS.seek 6ml @13:27:46.18 delay 2 seconds @13:27:48.20 CSV.dial :waste2 @13:27:48.75 CS.reconfigure :fast @13:27:48.80 CS.seek 0ml @13:28:03.53 CSV.dial CSR @13:28:04.02 Pulling 2ml of CSR.AqMeOH CSR.select :AqMeOH @13:28:04.13 CS.reconfigure :default @13:28:04.17 CS.seek 2ml @13:28:10.12 delay 5 seconds @13:28:15.14 CSR.select :air @13:28:15.29 CS.seek 5ml @13:28:23.90 delay 2 seconds @13:28:25.92 CSV.dial CTV @13:28:26.36 CS.seek 1ml @13:28:38.03 Before lysing Collection Puck Heater at 21.6C/39.0psia @13:28:38.05 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:28:38.06 CH.seek 70C,8:00 @13:30:46.81 CH.hold 10:00,70C @13:30:46.98 Begun lysing Collection Puck Heater at 68C/44.2psia @13:40:47.01 Ended lysing Collection Puck Heater at 70C/43.3psia @13:40:47.02 CS.seek 0ml @13:40:50.64 CTV.dial between spare and air,avoiding: :intake @13:40:51.69 CSV.dial :air @13:40:52.14 CS.seek 5ml @13:41:05.89 CSV.dial CTV @13:41:06.33 delay 2 seconds @13:41:08.34 CSV.dial :air @13:41:08.78 delay 2 seconds @13:41:10.80 CSV.dial CTV @13:41:11.26 delay 2 seconds @13:41:13.27 CSV.dial :air @13:41:13.72 delay 2 seconds @13:41:15.74 CS.seek 0ml @13:41:29.41 Puck @72.2psia after final lysis pressurization @13:41:29.42 Recovering Lysate into Collection syringe @13:41:29.43 CSV.dial CBV @13:41:30.45 CBV.dial CSV @13:41:30.81 CS.seek 5ml @13:41:44.36 delay 5 seconds @13:41:49.38 CTV.dial :air @13:41:49.89 CS.seek 7ml @13:41:55.76 delay 5 seconds @13:42:00.78 CSV.dial :air2 @13:42:01.22 CS.seek 10ml @13:42:09.89 CSV.dial between air and CTV,avoiding: CBV @13:42:11.07 Lysing Complete @13:42:11.13 Storing sh1 puck in Collection Clamp to tube 1 @13:42:11.29 SC.reconfigure :tight @13:42:11.33 SC.seek 1 @13:42:14.91 SC.configure :limp @13:42:15.10 Elbow.reconfigure :tight @13:42:15.14 Elbow.seek 1 @13:42:16.96 Forearm.reconfigure :tight @13:42:17.00 Forearm.seek 1 + 450 counts @13:42:18.02 SE.reconfigure :pick @13:42:18.07 SE.seek :beamBreak @13:42:20.97 SE.configure :default @13:42:21.11 Elbow.reconfigure :limp @13:42:21.16 Forearm.reconfigure :loose @13:42:21.19 Forearm.seek 1 @13:42:21.49 Elbow.hold @13:42:21.55 Elbow.reconfigure :tight @13:42:21.64 SE.seek 3.17 Pucks below Top Plate @13:42:22.20 Forearm.force -20 @13:42:22.24 Forearm.seek 1 + 269 counts @13:42:23.02 Forearm.reconfigure :tight @13:42:23.06 Forearm.seek 1 + 450 counts @13:42:23.25 SE.seek 4.17 Pucks below Top Plate @13:42:23.84 Forearm.reconfigure :loose @13:42:23.88 Forearm.seek :retract @13:42:24.83 Elbow.seek CC @13:42:26.55 Elbow.reconfigure :loose @13:42:26.63 Forearm.seek CC @13:42:27.62 Hand.close @13:42:28.17 CC.seek :open @13:42:32.89 Forearm.seek :retract @13:42:33.88 Elbow.reconfigure :tight @13:42:33.93 Elbow.seek 1 @13:42:35.68 Elbow.reconfigure :limp @13:42:35.72 Forearm.seek 1 @13:42:36.69 Elbow.hold @13:42:36.76 Elbow.reconfigure :tight @13:42:36.80 Hand.open @13:42:37.53 Forearm.force -20 @13:42:37.56 Forearm.seek 1 + 302 counts @13:42:37.93 Forearm.reconfigure :tight @13:42:37.97 Forearm.seek 1 + 450 counts @13:42:38.39 SE.seek :down @13:42:39.51
Forearm.reconfigure :loose @13:42:39.55 Forearm.seek :retract @13:42:40.83 SE.stop @13:42:40.87
Tranferring DA @13:42:40.88 Beginning samplePassC @13:42:40.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:42:41.07 Elbow.seek FlushPuck::Garage @13:42:42.85 Elbow.reconfigure :loose @13:42:42.94 Forearm.seek :garage @13:42:43.90 Hand.close @13:42:44.65 Forearm.seek :retract @13:42:45.68 Elbow.reconfigure :tight @13:42:45.73 Elbow.seek CC @13:42:48.64 Elbow.reconfigure :loose @13:42:48.73 Forearm.reconfigure :tight @13:42:48.77 Forearm.seek CC @13:42:50.03 CC.seek :closed @13:42:54.53 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.36 @13:42:54.60 Hand.open @13:42:55.22 Forearm.reconfigure :loose @13:42:55.26 Forearm.seek :retract @13:42:56.26 PSV.dial PSR @13:42:57.64 Pulling 1.5ml of PSR.flush @13:42:57.65 PSR.select :flush @13:42:57.77 PS.seek 1.5ml @13:43:13.84 delay 5 seconds @13:43:18.86 PSV.dial :waste1 @13:43:19.31 PS.seek 0ml @13:43:35.49 PSV.dial PSR @13:43:35.95 PSR.select :air @13:43:36.06 PS.seek 2.5ml @13:44:02.47 PRV.dial CBV,avoiding: :ab2 @13:44:02.49
CBV.dial PRV @13:44:04.18 PSV.dial :mixloop @13:44:05.21 CTV.dial :air,avoiding: :intake @13:44:05.23 PTV.dial :PRVmixing @13:44:06.56 PS.seek 0ml @13:44:32.93 PSV.dial :air @13:44:33.53 PS.seek 2.5ml @13:44:59.83 PSV.dial :mixloop @13:45:00.28 PS.seek 0ml @13:45:26.68 PSV.dial :air @13:45:27.14 PS.seek 2.5ml @13:45:53.55 PSV.dial :mixloop @13:45:54.01 PS.seek 0ml @13:46:20.40 Garaging FlushPuck in Collection Clamp @13:46:20.94 Elbow.reconfigure :tight @13:46:20.98 Elbow.seek CC @13:46:21.40 Elbow.reconfigure :loose @13:46:21.49 Forearm.seek CC @13:46:22.46 Hand.close @13:46:23.04 CC.seek :open @13:46:27.65 Forearm.seek :retract @13:46:28.65 Elbow.reconfigure :tight @13:46:28.69 Elbow.seek :garage @13:46:31.45 Elbow.reconfigure :loose @13:46:31.54 Forearm.reconfigure :tight @13:46:31.58 Forearm.seek :garage @13:46:32.68 Hand.open @13:46:33.34 Forearm.reconfigure :loose @13:46:33.39 Forearm.seek :retract @13:46:34.39 PSV.dial :mixloop @13:46:34.41 PTV.dial :PRVmixing @13:46:34.42 PRV.dial CBV @13:46:34.44
CBV.dial PRV @13:46:34.46 CTV.dial :air,avoiding: :intake @13:46:34.47 Loading lyfil puck from tube 2 into Collection Clamp @13:46:34.69 SC.reconfigure :tight @13:46:34.73 SC.seek 2 @13:46:38.30 SC.configure :limp @13:46:38.49 Elbow.reconfigure :tight @13:46:38.53 Elbow.seek 2 @13:46:40.41 Forearm.reconfigure :tight @13:46:40.45 Forearm.seek 2 + 450 counts @13:46:41.48 SE.reconfigure :pick @13:46:41.53 SE.seek :beamBreak @13:46:42.82 SE.configure :default @13:46:42.96 Elbow.seek 2 @13:46:43.13 Forearm.seek 2 + 300 counts @13:46:43.44 Forearm.reconfigure :loose @13:46:43.48 Hand.close @13:46:44.05 SE.seek :down @13:46:44.24
Forearm.seek :retract @13:46:45.01 SE.stop @13:46:45.15
Elbow.seek CC @13:46:46.76 Elbow.reconfigure :loose @13:46:46.85 Forearm.reconfigure :tight @13:46:46.89 Forearm.seek CC @13:46:48.15 CC.seek :closed @13:46:52.67 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.17 @13:46:52.68 Hand.open @13:46:53.34 Forearm.reconfigure :loose @13:46:53.39 Forearm.seek :retract @13:46:54.40 CTV.dial CSV,avoiding: :intake @13:46:55.76 CSV.dial CTV @13:46:56.08 PS.seek 2.5ml @13:47:22.55 CS.seek 4.5ml @13:47:37.38 delay 8 seconds @13:47:45.40 PSV.dial :air @13:47:45.86 delay 2 seconds @13:47:47.88 PSV.dial :waste2 @13:47:48.62 PS.seek 0ml @13:48:15.02 CBV.dial between PRV and waste1 @13:48:15.35 CSV.dial :air @13:48:15.81 CS.seek 10ml @13:48:30.66 CSV.dial CTV @13:48:31.11 CS.seek 0ml @13:48:57.69 CBV.dial :waste1 @13:48:57.98 delay 5 seconds @13:49:03.00 CTV.dial :air,avoiding: :intake @13:49:04.87 Storing lyfil puck in Collection Clamp to tube 1 @13:49:05.03 SC.reconfigure :tight @13:49:05.07 SC.seek 1 @13:49:08.65 SC.configure :limp @13:49:08.84 Elbow.reconfigure :tight @13:49:08.88 Elbow.seek 1 @13:49:10.68 Forearm.reconfigure :tight @13:49:10.72 Forearm.seek 1 + 450 counts @13:49:11.84 SE.reconfigure :pick @13:49:11.89 SE.seek :beamBreak @13:49:14.80 SE.configure :default @13:49:14.94 Elbow.reconfigure :limp @13:49:14.98 Forearm.reconfigure :loose @13:49:15.02 Forearm.seek 1 @13:49:15.30 Elbow.hold @13:49:15.36 Elbow.reconfigure :tight @13:49:15.45 SE.seek 4.17 Pucks below Top Plate @13:49:15.87 Forearm.force -20 @13:49:15.90 Forearm.seek 1 + 307 counts @13:49:16.28 Forearm.reconfigure :tight @13:49:16.32 Forearm.seek 1 + 450 counts @13:49:16.71 SE.seek 5.17 Pucks below Top Plate @13:49:17.26 Forearm.reconfigure :loose @13:49:17.30 Forearm.seek :retract @13:49:18.24 Elbow.seek CC @13:49:19.91 Elbow.reconfigure :loose @13:49:19.99 Forearm.seek CC @13:49:20.95 Hand.close @13:49:21.54 CC.seek :open @13:49:26.25 Forearm.seek :retract @13:49:27.22 Elbow.reconfigure :tight @13:49:27.27 Elbow.seek 1 @13:49:29.03 Elbow.reconfigure :limp @13:49:29.08 Forearm.seek 1 @13:49:30.03 Elbow.hold @13:49:30.09 Elbow.reconfigure :tight @13:49:30.13 Hand.open @13:49:30.89 Forearm.force -20 @13:49:30.93 Forearm.seek 1 + 315 counts @13:49:31.30 Forearm.reconfigure :tight @13:49:31.34 Forearm.seek 1 + 450 counts @13:49:31.80 SE.seek :down @13:49:32.92
Forearm.reconfigure :loose @13:49:32.96 Forearm.seek :retract @13:49:34.15 SE.stop @13:49:34.19
PSV.dial :air @13:49:34.93 PS.seek 2.5ml @13:50:01.24 delay 1 second @13:50:02.25 PSV.dial :mixloop @13:50:02.69 delay 1 second @13:50:03.70 PSV.dial :air @13:50:04.16 delay 1 second @13:50:05.17 PSV.dial :mixloop @13:50:05.63 delay 1 second @13:50:06.64 PSV.dial :air @13:50:07.10 delay 1 second @13:50:08.12 PS.seek 0ml @13:50:34.46 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @13:50:34.62 Elbow.seek FlushPuck::Garage @13:50:36.38 Elbow.reconfigure :loose @13:50:36.47 Forearm.seek :garage @13:50:37.45 Hand.close @13:50:38.14 Forearm.seek :retract @13:50:39.22 Elbow.reconfigure :tight @13:50:39.26 Elbow.seek PC @13:50:40.51 Elbow.reconfigure :loose @13:50:40.59 Forearm.reconfigure :tight @13:50:40.64 Forearm.seek PC @13:50:42.32 PC.seek :closed @13:50:46.96 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.63 @13:50:47.04 Hand.open @13:50:47.72 Forearm.reconfigure :loose @13:50:47.76 Forearm.seek :retract @13:50:48.70 PRV.dial :air @13:50:49.16 PSV.dial :mixloop @13:50:49.67 PS.seek 0.3ml @13:50:53.51 PSV.dial PSR @13:50:54.75 Pulling 0.4ml of PSR.air @13:50:54.76 PSR.select :air @13:50:54.78 PS.seek 0.7ml @13:50:59.64 delay 3 seconds @13:51:02.66 PSR.select :air @13:51:02.68 PS.seek 2.5ml @13:51:21.83 delay 3 seconds @13:51:24.85 PSV.dial :mixloop @13:51:25.89 PTV.dial :mixing @13:51:26.62 PBV.dial :waste2 @13:51:27.57 PS.seek 0ml @13:51:53.99 Garaging FlushPuck in Processing Clamp @13:51:54.05 Elbow.reconfigure :tight @13:51:54.10 Elbow.seek PC @13:51:54.21 Elbow.reconfigure :loose @13:51:54.30 Forearm.seek PC @13:51:55.23 Hand.close @13:51:55.78 PC.seek :open @13:52:00.50 Forearm.seek :retract @13:52:01.45 Elbow.reconfigure :tight @13:52:01.50 Elbow.seek :garage @13:52:02.85 Elbow.reconfigure :loose @13:52:02.94 Forearm.reconfigure :tight @13:52:02.98 Forearm.seek :garage @13:52:04.04 Hand.open @13:52:04.71 Forearm.reconfigure :loose @13:52:04.76 Forearm.seek :retract @13:52:05.74 Loading da puck from tube 2 into Processing Clamp @13:52:05.96 SC.reconfigure :tight @13:52:06.00 SC.seek 2 @13:52:09.55 SC.configure :limp @13:52:09.75 Elbow.reconfigure :tight @13:52:09.79 Elbow.seek 2 @13:52:11.64 Forearm.reconfigure :tight @13:52:11.68 Forearm.seek 2 + 450 counts @13:52:12.73 SE.reconfigure :pick @13:52:12.78 SE.seek :beamBreak @13:52:14.09 SE.configure :default @13:52:14.23 Elbow.seek 2 @13:52:14.40 Forearm.seek 2 + 300 counts @13:52:14.64 Forearm.reconfigure :loose @13:52:14.68 Hand.close @13:52:15.29 SE.seek :down @13:52:15.48
Forearm.seek :retract @13:52:16.35 SE.stop @13:52:16.41
Elbow.seek PC @13:52:18.93 Elbow.reconfigure :loose @13:52:19.02 Forearm.reconfigure :tight @13:52:19.06 Forearm.seek PC @13:52:20.23 PC.seek :closed @13:52:24.86 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.63 @13:52:24.87 Hand.open @13:52:25.96 Forearm.reconfigure :loose @13:52:26.01 Forearm.seek :retract @13:52:26.96 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @13:52:26.98 PH.seek 28C,5:00 @13:53:13.11 PH.hold 2:00:00,28C @13:53:13.15 delay 1:30 @13:54:43.19 PSR.select :air @13:54:43.21 PSV.dial PSR @13:54:44.32 Pulling 0.5ml of PSR.wash PSR.select :wash @13:54:44.44 PS.seek 0.5ml @13:54:50.36 delay 5 seconds @13:54:55.38 PSR.select :air @13:54:55.49 PS.seek 2ml @13:55:11.67 PTV.dial :puck @13:55:12.40 PSV.dial PTV @13:55:13.17 PBV.dial :waste1 @13:55:14.54 PS.seek 1.5ml @13:55:20.42 delay 3 seconds @13:55:23.44 PBV.dial between PSV and waste1 @13:55:23.80 PS.seek 0ml @13:55:39.89 delay 5:00 @14:00:39.91 recoverPSRreagent08 PTV.dial :puck @14:00:39.92 PBV.dial :waste1 @14:00:40.29 delay 30 seconds @14:01:10.31 PBV.dial between PSV and waste1 @14:01:10.68 PSR.select :air @14:01:10.69 PSV.dial PSR @14:01:11.46 PS.reconfigure :fast @14:01:11.50 PS.seek 2.5ml @14:01:35.63 PSV.dial PTV @14:01:36.38 delay 5 seconds @14:01:41.40 PSV.dial PSR @14:01:42.16 delay 5 seconds @14:01:47.18 PSV.dial PTV @14:01:47.94 delay 5 seconds @14:01:52.96 PSV.dial :air @14:01:53.84 PS.seek 0ml @14:02:17.75 PTV.dial :PRVmixing,via: PRV @14:02:19.08 PSV.dial :mixloop @14:02:19.52 PRV.dial :ab1 @14:02:20.27 PS.reconfigure :slow @14:02:20.32 PS.seek 0.15ml @14:02:24.03 delay 2 seconds @14:02:26.05 CBV.dial PRV @14:02:26.07
PRV.dial CBV @14:02:26.64 PS.seek 0.35ml @14:02:31.22 delay 2 seconds @14:02:33.24 CBV.dial between PRV and PSV @14:02:33.54 PRV.dial :air @14:02:33.99 PS.seek 0.9ml @14:02:45.75 PRV.dial :waste2 @14:02:46.48 PS.seek 0.5ml @14:02:55.10 addPMLReagent08 PSV.dial :air @14:02:55.57 PS.reconfigure :default @14:02:55.61 PS.seek 2ml @14:03:11.72 PSV.dial :PML @14:03:12.17 PTV.dial :mixing @14:03:12.91 PBV.dial :waste1 @14:03:13.33 PS.seek 1.85ml @14:03:15.88 PBV.dial between PSV and waste1 @14:03:16.26 PS.seek 0ml @14:03:35.94 delay 2 seconds @14:03:37.96 Beginning samplePassC @14:03:37.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:03:38.13 Elbow.reconfigure :tight @14:03:38.17 Elbow.seek FlushPuck::Garage @14:03:39.51 Elbow.reconfigure :loose @14:03:39.60 Forearm.seek :garage @14:03:40.61 Hand.close @14:03:41.39 Forearm.seek :retract @14:03:42.88 Elbow.reconfigure :tight @14:03:42.93 Elbow.seek CC @14:03:45.84 Elbow.reconfigure :loose @14:03:45.93 Forearm.reconfigure :tight @14:03:45.97 Forearm.seek CC @14:03:47.21 CC.seek :closed @14:03:51.74 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.47 @14:03:51.81 Hand.open @14:03:52.46 Forearm.reconfigure :loose @14:03:52.50 Forearm.seek :retract @14:03:53.46 PSV.dial PSR @14:03:54.57 Pulling 1.5ml of PSR.flush @14:03:54.58 PSR.select :flush @14:03:54.69 PS.seek 1.5ml @14:04:10.91 delay 5 seconds @14:04:15.93 PSV.dial :waste1 @14:04:16.38 PS.seek 0ml @14:04:32.69 PSV.dial PSR @14:04:33.14 PSR.select :air @14:04:33.25 PS.seek 2.5ml @14:04:59.66 PRV.dial CBV,avoiding: :ab2 @14:04:59.68
CBV.dial PRV @14:05:01.31 PSV.dial :mixloop @14:05:02.49 CTV.dial :air,avoiding: :intake @14:05:02.50 PTV.dial :PRVmixing @14:05:03.27 PS.seek 0ml @14:05:29.62 PSV.dial :air @14:05:30.21 PS.seek 2.5ml @14:05:56.56 PSV.dial :mixloop @14:05:57.00 PS.seek 0ml @14:06:23.28 PSV.dial :air @14:06:23.74 PS.seek 2.5ml @14:06:50.28 PSV.dial :mixloop @14:06:50.74 PS.seek 0ml @14:07:17.31 Garaging FlushPuck in Collection Clamp @14:07:17.38 Elbow.reconfigure :tight @14:07:17.42 Elbow.seek CC @14:07:17.83 Elbow.reconfigure :loose @14:07:17.91 Forearm.seek CC @14:07:18.88 Hand.close @14:07:19.52 CC.seek :open @14:07:24.13 Forearm.seek :retract @14:07:25.07 Elbow.reconfigure :tight @14:07:25.11 Elbow.seek :garage @14:07:28.03 Elbow.reconfigure :loose @14:07:28.12 Forearm.reconfigure :tight @14:07:28.16 Forearm.seek :garage @14:07:29.26 Hand.open @14:07:29.95 Forearm.reconfigure :loose @14:07:29.99 Forearm.seek :retract @14:07:30.98 delayUntil "14:11:37.94" @14:07:31.00 Whole Cell Archive sampling 100ml @14:07:31.02 Loading wcr puck from tube 2 into Collection Clamp @14:07:31.45 Elbow.reconfigure :tight @14:07:31.49 Elbow.seek 2 @14:07:33.41 Forearm.reconfigure :tight @14:07:33.46 Forearm.seek 2 + 450 counts @14:07:34.61 SE.reconfigure :pick @14:07:34.67 SE.seek :beamBreak @14:07:36.09 SE.configure :default @14:07:36.24 Elbow.seek 2 @14:07:36.42 Forearm.seek 2 + 300 counts @14:07:36.63 Forearm.reconfigure :loose @14:07:37.12 Hand.close @14:07:37.79 SE.seek :down @14:07:37.99 Forearm.seek :retract @14:07:38.89 SE.stop @14:07:38.95 Elbow.seek CC @14:07:40.59 Elbow.reconfigure :loose @14:07:40.69 Forearm.reconfigure :tight @14:07:40.73 Forearm.seek CC @14:07:41.99 CC.seek :closed @14:07:46.53 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.23 @14:07:46.55 Hand.open @14:07:47.20 Forearm.reconfigure :loose @14:07:47.25 Forearm.seek :retract @14:07:48.18 collectSample 100ml w/bubble point of 35psi @14:07:48.19 Priming 7ml @14:07:48.20 Intake.select :close @14:07:48.21 Exhaust.select :close @14:07:48.22 SS.hold @14:07:48.42 Calibrating pressure sensors @14:07:48.43 CTV.dial :air @14:07:48.44 SSV.dial :spit,avoiding: :exhaust @14:07:48.46 SS.reconfigure :fast @14:07:48.50 SS.seek 5ml @14:07:51.42 SSV.dial CBV @14:07:51.44 CBV.dial SSV @14:07:52.83 delay 3.8 seconds @14:07:56.75 SSV.dial :spit @14:07:58.11 SS.seek 0ml @14:08:00.77 SSV.dial CBV @14:08:02.14 CTV.dial :intake @14:08:02.90 SSV.dial CBV @14:08:02.92 CBV.dial SSV @14:08:02.94 Allowing 40s for 7.0ml of remaining 7.0ml @14:08:02.95 Intake.select :open @14:08:05.10 Intake @14.0psia, vacuum from 8.5 to 11.5psi @14:08:05.12 SS.reconfigure :inhaling 2.20ml/s @14:08:05.16 SS.seek 7ml @14:08:12.15 Stroke completed -- waiting for pressure to equalize ... @14:08:12.16 SS.reconfigure :exhale @14:08:12.20 SS.hold @14:08:38.70 Intake.select :close @14:08:38.85 SSV.dial :exhaust @14:08:39.63 Exhaust.select :open @14:08:39.74 SS.seek 0ml @14:08:43.87 Exhaust.select :close @14:08:43.98 Sampling 100.0ml @14:08:43.99 Intake.select :close @14:08:44.00 Exhaust.select :close @14:08:44.01 SS.reconfigure :default @14:08:44.05 SS.hold @14:08:44.24 CTV.dial :intake @14:08:44.25 SSV.dial CBV @14:08:44.28 CBV.dial SSV @14:08:45.06 Allowing 130s for 25.0ml of remaining 100.0ml @14:08:45.07 Intake.select :open @14:08:47.70 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:08:47.71 SS.reconfigure :inhaling 2.20ml/s @14:08:47.76 SS.seek 25ml @14:08:54.52 Vacuum too high -- waiting up to 40 seconds @14:08:54.53 SS.reconfigure :exhale @14:08:54.57 SS.hold @14:09:01.37 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:09:01.39 SS.reconfigure :inhaling 1.98ml/s @14:09:01.43 SS.seek 25ml @14:09:07.95 Vacuum too high -- waiting up to 40 seconds @14:09:07.96 SS.reconfigure :exhale @14:09:08.01 SS.hold @14:09:15.74 SS.reconfigure :inhaling 1.79ml/s @14:09:15.79 SS.seek 25ml @14:09:20.88 Stroke completed -- waiting for pressure to equalize ... @14:09:20.89 SS.reconfigure :exhale @14:09:20.94 SS.hold @14:09:27.43 Intake.select :close @14:09:27.57 SSV.dial :exhaust @14:09:28.34 Exhaust.select :open @14:09:28.45 SS.seek 0ml @14:09:37.26 Exhaust.select :close @14:09:37.37 SSV.dial CBV @14:09:37.38 CBV.dial SSV @14:09:38.15 Allowing 130s for 25.0ml of remaining 75.0ml @14:09:38.16 Intake.select :open @14:09:40.32 Intake @15.4psia, vacuum from 9.9 to 12.9psi @14:09:40.33 SS.reconfigure :inhaling 1.79ml/s @14:09:40.38 SS.seek 25ml @14:09:51.04 Vacuum too high -- waiting up to 40 seconds @14:09:51.06 SS.reconfigure :exhale @14:09:51.10 SS.hold @14:09:57.77 Intake @15.1psia, vacuum from 9.6 to 12.6psi @14:09:57.79 SS.reconfigure :inhaling 1.61ml/s @14:09:57.83 SS.seek 25ml @14:10:06.19 Stroke completed -- waiting for pressure to equalize ... @14:10:06.20 SS.reconfigure :exhale @14:10:06.25 SS.hold @14:10:17.78 Intake.select :close @14:10:17.93 SSV.dial :exhaust @14:10:18.71 Exhaust.select :open @14:10:18.82 SS.seek 0ml @14:10:28.13 Exhaust.select :close @14:10:28.24 SSV.dial CBV @14:10:28.26 CBV.dial SSV @14:10:29.06 Allowing 130s for 25.0ml of remaining 50.0ml @14:10:29.07 Intake.select :open @14:10:31.23 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:10:31.25 SS.reconfigure :inhaling 1.61ml/s @14:10:31.29 SS.seek 25ml @14:10:44.17 Vacuum too high -- waiting up to 40 seconds @14:10:44.18 SS.reconfigure :exhale @14:10:44.23 SS.hold @14:10:51.84 Intake @14.7psia, vacuum from 9.2 to 12.2psi @14:10:51.86 SS.reconfigure :inhaling 1.45ml/s @14:10:51.90 SS.seek 25ml @14:10:58.82 Stroke completed -- waiting for pressure to equalize ... @14:10:58.83 SS.reconfigure :exhale @14:10:58.87 SS.hold @14:11:08.63 Intake.select :close @14:11:08.77 SSV.dial :exhaust @14:11:09.54 Exhaust.select :open @14:11:09.65 SS.seek 0ml @14:11:18.68 Exhaust.select :close @14:11:18.79 SSV.dial CBV @14:11:18.80 CBV.dial SSV @14:11:19.57 Allowing 130s for 25.0ml of remaining 25.0ml @14:11:19.58 Intake.select :open @14:11:21.74 Intake @15.2psia, vacuum from 9.7 to 12.7psi @14:11:21.75 SS.reconfigure :inhaling 1.45ml/s @14:11:21.80 SS.seek 25ml @14:11:35.64 Vacuum too high -- waiting up to 40 seconds @14:11:35.65 SS.reconfigure :exhale @14:11:35.69 SS.hold @14:11:37.95 recoverPRVreagent08 @14:11:37.96 PTV.dial :mixing @14:11:38.76 PBV.dial :waste1 @14:11:39.13 delay 30 seconds @14:11:43.26 Intake @14.5psia, vacuum from 9.0 to 12.0psi @14:11:43.28 SS.reconfigure :inhaling 1.30ml/s @14:11:43.32 SS.seek 25ml @14:11:51.11 Stroke completed -- waiting for pressure to equalize ... @14:11:51.12 SS.reconfigure :exhale @14:11:51.17 SS.hold @14:11:59.74 Intake.select :close @14:11:59.89 SSV.dial :exhaust @14:12:01.16 Exhaust.select :open @14:12:01.27 SS.seek 0ml @14:12:09.15 PBV.dial between PSV and waste1 @14:12:09.52 PSV.dial :air @14:12:10.00 PS.reconfigure :fast @14:12:10.05 PS.seek 2.5ml @14:12:10.11 Exhaust.select :close @14:12:10.22 Sampled 100.0ml @14:12:10.24 Drying puck before fixing for 20:00 @14:12:10.25 CBV.dial :waste2b @14:12:11.01 delay 1:00 @14:12:34.86 PSV.dial :mixloop @14:12:35.35 delay 2 seconds @14:12:37.37 PSV.dial :air @14:12:37.98 delay 2 seconds @14:12:39.99 PSV.dial :mixloop @14:12:40.44 delay 2 seconds @14:12:42.46 PSV.dial :air @14:12:42.94 PS.seek 0ml @14:13:07.43 washCycleDA #1 of 2 @14:13:07.44 PSR.select :air @14:13:07.45 PSV.dial PSR @14:13:08.84 Pulling 0.5ml of PSR.wash @14:13:08.85 PSR.select :wash @14:13:08.96 PS.reconfigure :default @14:13:09.00 PS.seek 0.5ml @14:13:11.02 CSV.dial :air @14:13:11.49 CS.reconfigure :fast @14:13:11.54 CS.seek 10ml @14:13:14.96 delay 5 seconds @14:13:19.98 PSR.select :air @14:13:20.09 PS.seek 2ml @14:13:36.24 PTV.dial :puck @14:13:36.67 Pumping wcr puck from tube 2 up to 35psi... @14:13:36.69 CBV.dial SSV @14:13:36.99 PSV.dial PTV @14:13:37.75 PBV.dial :waste1 @14:13:38.02 CBV.dial between CSV and waste2b @14:13:38.17 PS.seek 1.5ml @14:13:38.98 CSV.dial between air and CTV @14:13:39.32 CS.seek 7.316ml @14:13:44.21 delay 3 seconds @14:13:46.37 CTV.dial CSV,avoiding: :air @14:13:46.39 CSV.dial CTV @14:13:47.22 CS.reconfigure :pressurize @14:13:47.27 CS.seek 0ml @14:13:47.31 PBV.dial between PSV and waste1 @14:13:47.69 PS.seek 0ml @14:14:01.03 CBV.dial :waste2b @14:14:01.36 CS.reconfigure :default @14:14:01.41 CS.seek 0ml @14:14:04.02 delay 2:00 @14:14:07.64 delay 1:00 @14:15:07.67 CTV.dial :air,avoiding: :intake @14:15:09.02 Fix #1 of 2 (with RNAlater) for 10:00 @14:15:09.03 CSV.dial CSR @14:15:09.65 Pulling 2ml of CSR.RNAlater @14:15:09.66 CSR.select :RNAlater @14:15:09.77 CS.seek 2ml @14:15:15.95 delay 5 seconds @14:15:20.97 CSR.select :air @14:15:21.08 CS.reconfigure :fast @14:15:21.12 CS.seek 10ml @14:15:41.23 Pumping wcr puck from tube 2 up to 35psi... @14:15:41.25 CBV.dial SSV @14:15:42.55 CBV.dial between CSV and waste2b @14:15:43.51 CSV.dial between air and CTV @14:15:44.10 CS.seek 7.324ml @14:15:51.21 CTV.dial CSV,avoiding: :air @14:15:51.24 CSV.dial CTV @14:15:52.61 CS.reconfigure :pressurize @14:15:52.66 CS.seek 0ml @14:16:04.05 recoverPSRreagent08 @14:16:04.06 PTV.dial :puck @14:16:04.07 PBV.dial :waste1 @14:16:04.44 delay 30 seconds @14:16:07.50 delay 10:00 @14:16:34.46 PBV.dial between PSV and waste1 @14:16:34.83 PSR.select :air @14:16:34.85 PSV.dial PSR @14:16:35.63 PS.reconfigure :fast @14:16:35.68 PS.seek 2.5ml @14:17:00.86 PSV.dial PTV @14:17:01.75 delay 5 seconds @14:17:06.77 PSV.dial PSR @14:17:07.52 delay 5 seconds @14:17:12.54 PSV.dial PTV @14:17:13.43 delay 5 seconds @14:17:18.44 PSV.dial :air @14:17:19.23 PS.seek 0ml @14:17:43.55 washCycleDA #2 of 2 @14:17:43.56 PSR.select :air @14:17:43.57 PSV.dial PSR @14:17:44.96 Pulling 0.5ml of PSR.wash @14:17:44.97 PSR.select :wash @14:17:45.09 PS.reconfigure :default @14:17:45.13 PS.seek 0.5ml @14:17:51.14 delay 5 seconds @14:17:56.16 PSR.select :air @14:17:56.28 PS.seek 2ml @14:18:12.45 PTV.dial :puck @14:18:12.47 PSV.dial PTV @14:18:13.33 PBV.dial :waste1 @14:18:13.76 PS.seek 1.5ml @14:18:19.64 delay 3 seconds @14:18:22.66 PBV.dial between PSV and waste1 @14:18:23.04 PS.seek 0ml @14:18:39.27 delay 2:00 @14:20:39.28 recoverPSRreagent08 @14:20:39.29 PTV.dial :puck @14:20:39.30 PBV.dial :waste1 @14:20:39.67 delay 30 seconds @14:21:09.70 PBV.dial between PSV and waste1 @14:21:10.06 PSR.select :air @14:21:10.08 PSV.dial PSR @14:21:10.83 PS.reconfigure :fast @14:21:10.87 PS.seek 2.5ml @14:21:35.63 PSV.dial PTV @14:21:36.40 delay 5 seconds @14:21:41.42 PSV.dial PSR @14:21:42.18 delay 5 seconds @14:21:47.20 PSV.dial PTV @14:21:47.97 delay 5 seconds @14:21:52.99 PSV.dial :air @14:21:53.75 PS.seek 0ml @14:22:17.92 getPMLReagent08 @14:22:17.93 PRV.dial :air @14:22:18.39 delay 5 seconds @14:22:23.41 PTV.dial :PRVmixing @14:22:24.75 delay 5 seconds @14:22:29.76 PRV.dial :ab2 @14:22:30.23 PTV.dial :PRVmixing @14:22:30.24 PSV.dial :mixloop @14:22:30.70 PS.reconfigure :default @14:22:30.75 PS.seek 0.3ml @14:22:34.63 delay 2 seconds @14:22:36.65 PRV.dial :air @14:22:37.16 PS.seek 0.55ml @14:22:40.73 addPMLReagent08 @14:22:40.74 PSV.dial :air @14:22:41.34 PS.seek 2ml @14:22:57.01 PSV.dial :PML @14:22:57.45 PTV.dial :mixing @14:22:58.21 PBV.dial :waste1 @14:22:58.63 PS.seek 1.85ml @14:23:01.01 PBV.dial between PSV and waste1 @14:23:01.39 PS.seek 0ml @14:23:21.04 delay 2 seconds @14:23:23.06 delay 8:00 @14:26:07.52 CBV.dial :waste2b @14:26:07.85 CS.reconfigure :default @14:26:07.90 CS.seek 0ml @14:26:13.04 delay 1:00 @14:27:13.07 CTV.dial :air,avoiding: :intake @14:27:14.43 Fix #2 of 2 (with RNAlater) for 10:00 @14:27:14.44 CSV.dial CSR @14:27:14.93 Pulling 1.999ml of CSR.RNAlater @14:27:14.94 CSR.select :RNAlater @14:27:15.05 CS.seek 2ml @14:27:21.03 delay 5 seconds @14:27:26.05 CSR.select :air @14:27:26.16 CS.reconfigure :fast @14:27:26.21 CS.seek 10ml @14:27:46.41 Pumping wcr puck from tube 2 up to 35psi... @14:27:46.42 CBV.dial SSV @14:27:47.72 CBV.dial between CSV and waste2b @14:27:48.68 CSV.dial between air and CTV @14:27:49.31 CS.seek 7.307ml @14:27:56.41 CTV.dial CSV,avoiding: :air @14:27:56.43 CSV.dial CTV @14:27:57.83 CS.reconfigure :pressurize @14:27:57.88 CS.seek 0ml @14:28:12.68 delay 10:00 @14:31:23.07 recoverPRVreagent08 @14:31:23.08 PTV.dial :mixing @14:31:23.09 PBV.dial :waste1 @14:31:23.45 delay 30 seconds @14:31:53.48 PBV.dial between PSV and waste1 @14:31:54.31 PSV.dial :air @14:31:54.79 PS.reconfigure :fast @14:31:54.83 PS.seek 2.5ml @14:32:19.40 PSV.dial :mixloop @14:32:19.89 delay 2 seconds @14:32:21.91 PSV.dial :air @14:32:22.50 delay 2 seconds @14:32:24.52 PSV.dial :mixloop @14:32:24.97 delay 2 seconds @14:32:26.98 PSV.dial :air @14:32:27.46 PS.seek 0ml @14:32:51.56 washCycleDA #1 of 3 @14:32:51.57 PSR.select :air @14:32:51.59 PSV.dial PSR @14:32:52.97 Pulling 0.5ml of PSR.wash @14:32:52.98 PSR.select :wash @14:32:53.10 PS.reconfigure :default @14:32:53.14 PS.seek 0.5ml @14:32:59.13 delay 5 seconds @14:33:04.15 PSR.select :air @14:33:04.26 PS.seek 2ml @14:33:20.59 PTV.dial :puck @14:33:21.34 PSV.dial PTV @14:33:22.09 PBV.dial :waste1 @14:33:22.52 PS.seek 1.5ml @14:33:28.53 delay 3 seconds @14:33:31.55 PBV.dial between PSV and waste1 @14:33:31.93 PS.seek 0ml @14:33:48.06 delay 2:00 @14:35:48.08 recoverPSRreagent08 @14:35:48.09 PTV.dial :puck @14:35:48.10 PBV.dial :waste1 @14:35:48.47 delay 30 seconds @14:36:18.49 PBV.dial between PSV and waste1 @14:36:18.85 PSR.select :air @14:36:18.87 PSV.dial PSR @14:36:19.64 PS.reconfigure :fast @14:36:19.68 PS.seek 2.5ml @14:36:44.12 PSV.dial PTV @14:36:44.88 delay 5 seconds @14:36:49.90 PSV.dial PSR @14:36:50.67 delay 5 seconds @14:36:55.69 PSV.dial PTV @14:36:56.57 delay 5 seconds @14:37:01.59 PSV.dial :air @14:37:02.37 PS.seek 0ml @14:37:26.37 washCycleDA #2 of 3 @14:37:26.38 PSR.select :air @14:37:26.39 PSV.dial PSR @14:37:27.78 Pulling 0.5ml of PSR.wash @14:37:27.79 PSR.select :wash @14:37:27.90 PS.reconfigure :default @14:37:27.95 PS.seek 0.5ml @14:37:33.87 delay 5 seconds @14:37:38.89 PSR.select :air @14:37:39.00 PS.seek 2ml @14:37:55.11 PTV.dial :puck @14:37:55.13 PSV.dial PTV @14:37:55.99 PBV.dial :waste1 @14:37:56.41 PS.seek 1.5ml @14:38:02.37 delay 3 seconds @14:38:05.38 PBV.dial between PSV and waste1 @14:38:05.76 PS.seek 0ml @14:38:12.70 CBV.dial :waste2b @14:38:13.03 CS.reconfigure :default @14:38:13.07 CS.seek 0ml @14:38:18.21 delay 1:00 @14:38:21.90 delay 2:00 @14:39:18.24 CTV.dial :air,avoiding: :intake @14:40:21.92 recoverPSRreagent08 @14:40:21.93 PTV.dial :puck @14:40:21.94 PBV.dial :waste1 @14:40:22.30 delay 30 seconds @14:40:52.33 PBV.dial between PSV and waste1 @14:40:52.69 PSR.select :air @14:40:52.71 PSV.dial PSR @14:40:53.46 PS.reconfigure :fast @14:40:53.51 PS.seek 2.5ml @14:41:18.08 PSV.dial PTV @14:41:18.85 delay 5 seconds @14:41:23.87 PSV.dial PSR @14:41:24.63 delay 5 seconds @14:41:29.65 PSV.dial PTV @14:41:30.53 delay 5 seconds @14:41:35.55 PSV.dial :air @14:41:36.34 PS.seek 0ml @14:42:00.46 washCycleDA #3 of 3 @14:42:00.47 PSR.select :air @14:42:00.48 PSV.dial PSR @14:42:01.86 Pulling 0.5ml of PSR.wash @14:42:01.87 PSR.select :wash @14:42:01.99 PS.reconfigure :default @14:42:02.03 PS.seek 0.5ml @14:42:07.93 delay 5 seconds @14:42:12.94 PSR.select :air @14:42:13.06 PS.seek 2ml @14:42:29.23 PTV.dial :puck @14:42:29.25 PSV.dial PTV @14:42:29.99 PBV.dial :waste1 @14:42:30.40 PS.seek 1.5ml @14:42:36.30 delay 3 seconds @14:42:39.32 PBV.dial between PSV and waste1 @14:42:39.70 PS.seek 0ml @14:42:55.80 delay 2:00 @14:44:55.83 recoverPSRreagent08 PTV.dial :puck @14:44:55.84 PBV.dial :waste1 @14:44:56.20 delay 30 seconds @14:45:26.22 PBV.dial between PSV and waste1 @14:45:27.06 PSR.select :air @14:45:27.08 PSV.dial PSR @14:45:27.85 PS.reconfigure :fast @14:45:27.89 PS.seek 2.5ml @14:45:52.36 PSV.dial PTV @14:45:53.12 delay 5 seconds @14:45:58.14 PSV.dial PSR @14:45:58.90 delay 5 seconds @14:46:03.92 PSV.dial PTV @14:46:04.69 delay 5 seconds @14:46:09.71 PSV.dial :air @14:46:10.61 PS.seek 0ml @14:46:34.76 Gate.power :camera,:ON @14:46:34.78 Mixing DA Substrates PSR.select :air @14:46:34.80 PSV.dial PSR @14:46:36.40 Pulling 0.25ml of PSR.sub1 @14:46:36.43 PSR.select :sub1 @14:46:36.59 PS.reconfigure :default @14:46:36.69 PS.seek 0.25ml @14:46:40.11 delay 5 seconds @14:46:45.18 Pulling 0.25ml of PSR.sub2 @14:46:45.19 PSR.select :sub2 @14:46:45.31 PS.seek 0.5ml @14:46:48.73 delay 5 seconds @14:46:53.75 PSR.select :air @14:46:53.86 PS.seek 2ml @14:47:10.04 PTV.dial :puck @14:47:10.05 PSV.dial PTV @14:47:10.85 PBV.dial :waste1 @14:47:11.29 PS.seek 1.5ml @14:47:17.20 delay 3 seconds @14:47:20.22 PBV.dial between PSV and waste1 @14:47:20.61 PS.seek 0ml @14:47:36.73 delay 10 seconds @14:47:46.76 recoverPSRreagent08 @14:47:46.77 PTV.dial :puck @14:47:46.78 PBV.dial :waste1 @14:47:47.18 delay 30 seconds @14:48:17.21 PBV.dial between PSV and waste1 @14:48:17.59 PSR.select :air @14:48:17.61 PSV.dial PSR @14:48:18.42 PS.reconfigure :fast @14:48:18.47 PS.seek 2.5ml @14:48:44.38 PSV.dial PTV @14:48:45.32 delay 5 seconds @14:48:50.35 PSV.dial PSR @14:48:51.10 delay 5 seconds @14:48:56.12 PSV.dial PTV @14:48:57.04 delay 5 seconds @14:49:02.06 PSV.dial :air @14:49:02.89 PS.seek 0ml @14:49:28.20 Additional air clear @14:49:28.21 PSR.select :air @14:49:28.22 PSV.dial PSR @14:49:29.65 Pulling 2ml of PSR.air @14:49:29.66 PSR.select :air @14:49:29.69 PS.reconfigure :default @14:49:29.73 PS.seek 2ml @14:49:51.01 delay 5 seconds @14:49:56.03 PTV.dial :puck @14:49:56.04 PSV.dial PTV @14:49:56.84 PBV.dial :waste1 @14:49:57.26 PS.seek 1.5ml @14:50:03.29 delay 3 seconds @14:50:06.31 PBV.dial between PSV and waste1 @14:50:06.68 delay 1 second @14:50:07.70 PSV.dial :waste1 @14:50:08.83 PS.seek 0ml @14:50:25.03 Processing Puck Heater at 28.1C before imaging @14:50:25.04 PH.coast @14:50:25.15 Imaging da puck in Processing Clamp while storing it to tube 1 @14:50:25.31 SC.reconfigure :tight @14:50:25.36 SC.seek 1 @14:50:28.96 SC.configure :limp @14:50:29.15 Elbow.reconfigure :tight @14:50:29.19 Elbow.seek 1 @14:50:30.88 Forearm.reconfigure :tight @14:50:30.92 Forearm.seek 1 + 450 counts @14:50:32.03 SE.reconfigure :pick @14:50:32.08 SE.seek :beamBreak @14:50:34.93 SE.configure :default @14:50:35.07 Elbow.reconfigure :limp @14:50:35.12 Forearm.reconfigure :loose @14:50:35.16 Forearm.seek 1 @14:50:35.45 Elbow.hold @14:50:35.51 Elbow.reconfigure :tight @14:50:35.60 SE.seek 5.15 Pucks below Top Plate @14:50:36.06 Forearm.force -20 @14:50:36.09 Forearm.seek 1 + 281 counts @14:50:36.36 Forearm.reconfigure :tight @14:50:36.41 Forearm.seek 1 + 450 counts @14:50:36.65 SE.seek 6.15 Pucks below Top Plate @14:50:37.15 Forearm.reconfigure :loose @14:50:37.20 Forearm.seek :retract @14:50:38.11 Elbow.seek PC @14:50:40.63 Elbow.reconfigure :loose @14:50:41.14 Forearm.seek PC @14:50:42.15 Hand.close @14:50:42.86 PC.seek :open @14:50:47.60 Forearm.seek :retract @14:50:48.58 Elbow.reconfigure :tight @14:50:48.62 Elbow.seek 1 @14:50:51.25 Forearm.reconfigure :tight @14:50:51.30 Forearm.seek 1 + 450 counts @14:50:52.45 Hand.open @14:50:53.30 SE.seek 5.8 Pucks below Top Plate @14:50:53.57 SE.reconfigure :seal @14:50:53.62 SE.seek 4.83 Pucks below Top Plate @14:50:54.20 Lights, camera, ACTION! @14:50:54.23 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21nov0914h100ml.tif @14:51:13.67 Waiting >20 seconds for da to relinquish FlushPuck @14:51:47.87 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 50.064 seconds /var/log/esp/da21nov0914h100ml.tif: TIFF Upload Complete @14:51:47.90 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21nov0914h100ml15s.tif @14:52:03.51 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21nov0914h100ml15s.tif: TIFF Upload Complete @14:52:03.54 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21nov0914h100ml30s.tif @14:52:34.19 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21nov0914h100ml30s.tif: TIFF Upload Complete @14:52:34.21 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21nov0914h100ml70s.tif @14:53:45.99 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21nov0914h100ml70s.tif: TIFF Upload Complete @14:53:46.00 Gate.power :camera,:OFF @14:53:46.02 DAfans.email "50.064 sec auto-exposure",Subject: "da21nov0914h100ml images!" @14:53:46.28 Elbow.seek 1 @14:53:46.48 Forearm.seek 1 + 450 counts @14:53:46.68 SE.reconfigure :gently @14:53:46.74 SE.seek 5.55 Pucks below Top Plate @14:53:47.47 Elbow.reconfigure :limp @14:53:47.52 Forearm.reconfigure :loose @14:53:47.57 Forearm.seek 1 @14:53:47.83 Elbow.hold @14:53:47.90 Elbow.reconfigure :tight @14:53:47.96 SE.reconfigure :default @14:53:48.01 SE.seek 5.73 Pucks below Top Plate @14:53:48.46 Forearm.force -20 @14:53:48.50 Forearm.seek 1 + 295 counts @14:53:48.87 Forearm.reconfigure :tight @14:53:48.92 Forearm.seek 1 + 450 counts @14:53:49.22 SE.seek :down @14:53:50.37 Forearm.reconfigure :loose @14:53:50.41 Forearm.seek :retract @14:53:51.56 SE.stop @14:53:51.68 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:53:51.96 Elbow.seek FlushPuck::Garage @14:53:54.10 Elbow.reconfigure :loose @14:53:54.28 Forearm.seek :garage @14:53:55.32 Hand.close @14:53:56.23 Forearm.seek :retract @14:53:57.38 Elbow.reconfigure :tight @14:53:57.46 Elbow.seek PC @14:53:58.84 Elbow.reconfigure :loose @14:53:59.01 Forearm.reconfigure :tight @14:53:59.09 Forearm.seek PC @14:54:00.70 PC.seek :closed @14:54:05.57 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.71 @14:54:06.79 Hand.open @14:54:07.50 Forearm.reconfigure :loose @14:54:07.58 Forearm.seek :retract @14:54:08.58 PSR.select :air @14:54:08.64 Storing wcr puck in Collection Clamp to tube 1 @14:54:08.68 PSV.dial PSR @14:54:09.22 SC.reconfigure :tight @14:54:09.32 SC.seek 1 @14:54:09.46 SC.configure :limp @14:54:09.78 Pulling 0.5ml of PSR.flush @14:54:09.84 PSR.select :flush @14:54:10.16 Elbow.reconfigure :tight @14:54:10.24 Elbow.seek 1 @14:54:10.36 PS.seek 0.5ml @14:54:13.22 Forearm.reconfigure :tight @14:54:13.34 Forearm.seek 1 + 450 counts @14:54:14.50 SE.reconfigure :pick @14:54:14.60 SE.seek :beamBreak @14:54:16.31 delay 5 seconds @14:54:17.41 SE.configure :default @14:54:17.76 Elbow.reconfigure :limp @14:54:17.85 Forearm.reconfigure :loose @14:54:17.94 Forearm.seek 1 @14:54:18.23 Elbow.hold @14:54:18.36 Elbow.reconfigure :tight @14:54:18.58 SE.seek 6.11 Pucks below Top Plate @14:54:19.19 Forearm.force -20 @14:54:19.30 Forearm.seek 1 + 292 counts @14:54:19.82 Forearm.reconfigure :tight @14:54:19.90 Forearm.seek 1 + 450 counts @14:54:20.24 SE.seek 7.11 Pucks below Top Plate @14:54:21.05 Forearm.reconfigure :loose @14:54:21.19 Forearm.seek :retract @14:54:21.34 PSR.select :air @14:54:21.50 PS.seek 2.5ml @14:54:22.23 Elbow.seek CC @14:54:24.00 Elbow.reconfigure :loose @14:54:24.21 Forearm.seek CC @14:54:25.21 Hand.close @14:54:25.88 CC.seek :open @14:54:30.92 Forearm.seek :retract @14:54:31.94 Elbow.reconfigure :tight @14:54:32.03 Elbow.seek 1 @14:54:33.97 Elbow.reconfigure :limp @14:54:34.07 Forearm.seek 1 @14:54:35.10 Elbow.hold @14:54:35.23 Elbow.reconfigure :tight @14:54:35.33 Hand.open @14:54:36.27 Forearm.force -20 @14:54:36.35 Forearm.seek 1 + 320 counts @14:54:36.85 Forearm.reconfigure :tight @14:54:36.94 Forearm.seek 1 + 450 counts @14:54:37.41 SE.seek :down @14:54:38.67 Forearm.reconfigure :loose @14:54:38.76 Forearm.seek :retract @14:54:39.71 SE.stop @14:54:39.85
Starting finalPurge @14:54:42.78 delay 1 second @14:54:43.81 PRV.dial :waste2,avoiding: :ab2 @14:54:45.72 PTV.dial :PRVmixing @14:54:47.10 PSV.dial :mixloop @14:54:48.21 PS.seek :empty @14:54:59.72
Waiting >20 seconds for psFlush to relinquish FlushPuck @14:55:14.71 PSR.select :air @14:55:15.69 PSV.dial PSR @14:55:16.99 Pulling 0.5ml of PSR.flush @14:55:17.01 PSR.select :flush @14:55:17.19 PS.seek 0.5ml @14:55:23.18 delay 5 seconds @14:55:28.21 PSR.select :air @14:55:28.36 PS.seek 2.5ml @14:55:49.71 delay 1 second @14:55:50.75 PBV.dial :waste1 @14:55:51.14 PTV.dial :mixing @14:55:51.93 PSV.dial :mixloop @14:55:53.04 PS.seek :empty @14:56:19.49 PSV.dial PSR @14:56:20.60 PSR.select :flush @14:56:20.77 PS.seek 1ml @14:56:31.80 PSR.select :air @14:56:32.00 PS.seek 2.5ml @14:56:42.95 upsync @14:56:48.22 PSV.dial PTV @14:56:48.98 PTV.dial :puck @14:56:49.73 PBV.dial :waste1 @14:56:49.75 PS.seek 0ml @14:57:16.15 PSV.dial PSR @14:57:16.92 PSR.select :flush @14:57:17.04 PS.seek 0.5ml @14:57:23.00 PSR.select :air @14:57:23.11 PS.seek 2.5ml @14:57:44.43 PSV.dial PTV @14:57:45.20 PBV.dial PSV @14:57:45.77 PS.seek 0ml @14:58:12.15 PSV.dial PBV @14:58:12.63 PS.seek 2.5ml @14:58:39.18 PSV.dial :waste1 @14:58:40.05 PS.seek 0ml @14:59:06.40 PSV.dial PSR @14:59:06.85 PSR.select :air @14:59:06.88 PS.seek 2.5ml @14:59:33.43 PSV.dial PTV @14:59:34.18 PBV.dial PSV @14:59:34.20 PS.seek 0ml @15:00:00.81 PSV.dial PBV @15:00:01.28 PS.seek 2.5ml @15:00:27.65 PSV.dial :waste1 @15:00:28.52 PS.seek 0ml @15:00:54.90 PSV.dial PSR @15:00:55.37 PSR.select :flush @15:00:55.48 PS.seek 1ml @15:01:06.53 PSR.select :air @15:01:06.64 PS.seek 2.5ml @15:01:22.87 PSV.dial PTV @15:01:23.60 PTV.dial :puck @15:01:23.62 PBV.dial :waste1 @15:01:24.17 PS.seek 0ml @15:01:50.52 PSV.dial PSR @15:01:51.27 PSR.select :flush @15:01:51.39 PS.seek 0.5ml @15:01:57.27 PSR.select :air @15:01:57.38 PS.seek 2.5ml @15:02:18.62 PSV.dial PTV @15:02:19.38 PBV.dial PSV @15:02:19.95 PS.seek 0ml @15:02:46.51 PSV.dial PBV @15:02:46.98 PS.seek 2.5ml @15:03:13.40 PSV.dial :waste1 @15:03:14.16 PS.seek 0ml @15:03:40.74 PSV.dial PSR @15:03:41.21 PSR.select :air @15:03:41.23 PS.seek 2.5ml @15:04:07.55 PSV.dial PTV @15:04:08.29 PBV.dial PSV @15:04:08.31 PS.seek 0ml @15:04:34.68 PSV.dial PBV @15:04:35.14 PS.seek 2.5ml @15:05:01.52 PSV.dial :waste1 @15:05:02.26 PS.seek 0ml @15:05:28.81
Loading FlushPuck into Collection Clamp from Processing Clamp @15:05:28.97 Elbow.seek PC @15:05:31.46 Elbow.reconfigure :loose @15:05:31.55 Forearm.seek PC @15:05:32.47 Hand.close @15:05:33.10 PC.seek :open @15:05:37.78 Forearm.seek :retract @15:05:38.74 Elbow.reconfigure :tight @15:05:39.23 Elbow.seek CC @15:05:42.80 Elbow.reconfigure :loose @15:05:42.88 Forearm.reconfigure :tight @15:05:42.93 Forearm.seek CC @15:05:44.25 CC.seek :closed @15:05:48.78 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.44 @15:05:48.86 Hand.open @15:05:49.53 Forearm.reconfigure :loose @15:05:49.57 Forearm.seek :retract @15:05:50.52 Flushing the Collection Valve::Manifold @15:05:50.64 CSV.dial CSR @15:05:51.12 Pulling 5ml of CSR.flush @15:05:51.13 CSR.select :flush @15:05:51.24 CS.seek 5ml @15:06:04.81 delay 5 seconds @15:06:09.82 CSR.select :air @15:06:09.97 CS.reconfigure :fast @15:06:10.01 CS.seek 7.499ml @15:06:16.83 CSV.dial :waste @15:06:18.14 CS.reconfigure :default @15:06:18.18 CS.seek 0ml @15:06:38.21 Flushing lower manifold's waste1 port @15:06:38.33 CSV.dial :flush @15:06:38.78 CS.reconfigure :fast @15:06:38.82 CS.seek 5ml @15:06:51.68 delay 5 seconds @15:06:56.70 CSV.dial :air @15:06:57.24 CS.seek 7ml @15:07:02.93 CSV.dial CTV @15:07:02.95
CTV.dial CSV @15:07:04.33 CBV.dial :waste1 @15:07:05.09 CS.seek 0ml @15:07:21.96 Flushing lower manifold's waste2 port @15:07:22.07 CSV.dial :flush @15:07:22.82 CS.seek 5ml @15:07:35.80 delay 5 seconds @15:07:40.82 CSV.dial :air @15:07:41.35 CS.seek 7ml @15:07:46.99 CSV.dial CTV @15:07:47.01
CTV.dial CSV @15:07:47.46 CBV.dial :waste2 @15:07:48.53 CS.seek 0ml @15:08:05.49 Flushing lower manifold's waste2b port @15:08:05.61 CSV.dial :flush @15:08:06.47 CS.seek 5ml @15:08:19.43 delay 5 seconds @15:08:24.44 CSV.dial :air @15:08:24.94 CS.seek 7ml @15:08:30.56 CSV.dial CTV @15:08:30.57
CTV.dial CSV @15:08:31.15 CBV.dial :waste2b @15:08:32.20 CS.seek 0ml @15:08:49.08 Disinfecting external Intake with kill @15:08:49.20 CSV.dial CSR @15:08:49.72 Pulling 8ml of CSR.kill CSR.select :kill @15:08:49.83 CS.seek 8ml @15:09:10.24 delay 5 seconds @15:09:15.26 CSR.select :air @15:09:15.37 CS.seek 10ml @15:09:20.99 CSV.dial CBV @15:09:21.01
CBV.dial CSV @15:09:22.08 CTV.dial :intake @15:09:22.86 Intake.select :open @15:09:22.97 CS.seek 2ml @15:09:42.20 Intake.select :close @15:09:42.30 CTV.dial :air @15:09:43.07 CS.reconfigure :default @15:09:43.11 CS.seek 0ml @15:09:49.08 CSV.dial CSR @15:09:50.15 Pulling 5ml of CSR.flush @15:09:50.16 CSR.select :flush @15:09:50.27 CS.seek 5ml @15:10:04.02 delay 5 seconds @15:10:09.04 CSR.select :air @15:10:09.15 CS.seek 10ml @15:10:22.74 CSV.dial CBV @15:10:23.74 CS.seek 0ml @15:10:50.11 Garaging FlushPuck in Collection Clamp @15:10:50.18 Elbow.reconfigure :tight @15:10:50.22 Elbow.seek CC @15:10:50.57 Elbow.reconfigure :loose @15:10:50.65 Forearm.seek CC @15:10:52.05 Hand.close @15:10:52.72 CC.seek :open @15:10:57.32 Forearm.seek :retract @15:10:58.27 Elbow.reconfigure :tight @15:10:58.31 Elbow.seek :garage @15:11:01.18 Elbow.reconfigure :loose @15:11:01.27 Forearm.reconfigure :tight @15:11:01.31 Forearm.seek :garage @15:11:02.29 Hand.open @15:11:02.90 Forearm.reconfigure :loose @15:11:02.94 Forearm.seek :retract @15:11:03.89 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 13:15:53 @15:11:03.94 Asleep after completing DA_WCR_110921 assays @15:11:03.96 GoodNews.email "{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:05:14, 16% flow, 22.5C, 13% humidity, 15.2psia, 13.486V, 0.375A, 0.363A avg, 5.06W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_110921 assays" @15:11:37.21
Uploading Logs @15:13:19.28 upsync @13:42:20.25UTC27-Dec-21
ESP2 v3.41 I2C Gateway v3.20 for core bus @13:42:22.87 Configuration for short puck jake 1/11/21 by brent@mbari.org /home/esp/esp2/type/shallow/jake/configure.rb @13:42:26.74 Polling Can every 10:00 @13:42:26.80 Cleanly exited at 13:39:52UTC27-Dec-21 @13:42:26.84 Loading /home/esp/esp2/mission/DA_WCR_122621.rb mission @13:42:26.89 GoodNews.email "Starting DA_WCR_122621 w/pucks in tube 2\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:42:26, 18% flow, 15.8C, 35% humidity, 14.6psia, 13.632V, 0.345A, 0.333A avg, 4.70W",Subject: "DA_WCR_122621 begun" @13:42:26.93
Preparing to sleep until + 00:00:05 @13:42:26.96 Asleep til + 00:00:05 @13:42:26.97 Continuing at 13:42 @13:42:27.26 Gate.power :core,:OFF @13:42:27.27 Gate.power :raw,:OFF @13:42:27.28 Shutdown @13:42:31.86
Powering core bus @13:42:32.23 GoodNews.email "Then, in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_122621.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:42:27, 18% flow, 15.8C, 35% humidity, 14.6psia, 13.632V, 0.345A, 0.333A avg, 4.70W",Subject: "Asleep until 13:42" @13:42:37.48
Gate.power :raw,:ON @13:42:38.96 CS.configure :default @13:42:39.16 PS.configure :default @13:42:39.36 SS.configure :default @13:42:39.54 CC.configure :default @13:42:39.72 PC.configure :default @13:42:39.92 Forearm.configure :loose @13:42:40.10 Elbow.configure :loose @13:42:40.42 SC.configure :loose @13:42:40.60 SE.configure :default @13:42:40.68 CH.coast @13:42:40.81 CH.configure :default @13:42:40.87 PH.coast @13:42:40.97 PH.configure :default @13:42:41.05 All dwarf controllers running firmware version 3.85 @13:42:41.19 Valve.reset @13:42:41.49 Intake.select :close @13:42:41.70 Exhaust.select :close @13:42:41.74 CTV.dial :air @13:42:41.96 PSV.dial :air @13:42:42.09 CSV.dial :waste1 @13:42:42.34
Forearm.reconfigure :loose @13:42:42.38 PS.reconfigure :default @13:42:42.56
Forearm.home.seek :retract @13:42:42.64 PS.home.seek 0ml @13:42:42.80 SSV.dial :spit,avoiding: :exhaust @13:42:43.02 CS.reconfigure :fast @13:42:43.15 SS.reconfigure :default @13:42:43.30 CS.home.seek 0ml @13:42:43.35 SS.home.seek 0ml @13:42:43.53 Hand.close @13:42:43.57
Elbow.jog 1500 @13:42:44.13 Elbow.reconfigure :loose @13:42:44.33 Elbow.home.seek :clear @13:42:47.68 SE.reconfigure :default @13:42:47.76 SE.home.seek :down @13:42:48.49 SC.reconfigure :tight @13:42:48.57 SC.home.seek :initial @13:42:51.06 PS.stop @13:43:07.62
SC.configure :limp @13:43:07.70 Forearm.seek :retract @13:43:07.84 Elbow.seek :hammer @13:43:09.03 Forearm.reconfigure :tight @13:43:09.11 Forearm.seek retracted @13:43:09.66 CC.reconfigure :default @13:43:09.69 PC.reconfigure :default @13:43:09.85
CC.home.seek :open @13:43:09.89 PC.home.seek :open @13:43:13.93
Phase [initialPurge; da [100, 100] #da and wsr are run simultanelously] @13:43:13.95 Notice.email "At 13:43:13 began in tube 2: initialPurge; da [100, 100] #da and wsr are run simultanelously\n/home/esp/esp2/mission/DA_WCR_122621.rb:14\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:43:13, 18% flow, 15.8C, 35% humidity, 14.6psia, 13.637V, 0.345A, 0.327A avg, 4.70W",Subject: "Started DA_WCR_122621 phase initialPurge; da [100, 100] #da and wsr are run simultanelously" @13:43:13.99
Starting initialPurge @13:43:14.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:43:14.18 Hand.open @13:43:15.05 Elbow.reconfigure :tight @13:43:15.13 Elbow.seek FlushPuck::Garage @13:43:17.14 Elbow.reconfigure :loose @13:43:17.34 Forearm.reconfigure :loose @13:43:17.46 Forearm.seek :garage @13:43:18.48 Hand.close @13:43:19.32 Forearm.seek :retract @13:43:20.29 Elbow.reconfigure :tight @13:43:20.38 Elbow.seek CC @13:43:23.43 Elbow.reconfigure :loose @13:43:23.65 Forearm.reconfigure :tight @13:43:23.73 Forearm.seek CC @13:43:25.32 CC.seek :closed @13:43:30.00 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @13:43:30.14 Hand.open @13:43:30.93 Forearm.reconfigure :loose @13:43:31.01 Forearm.seek :retract @13:43:32.02 Flushing the Collection Valve::Manifold @13:43:32.24 CSV.dial CSR @13:43:33.68 Pulling 5ml of CSR.flush @13:43:33.70 CSR.select :flush @13:43:33.90 CS.reconfigure :default @13:43:33.98 CS.seek 5ml @13:43:41.75 upsync @13:43:47.59
delay 5 seconds @13:43:52.61 CSR.select :air @13:43:52.76 CS.reconfigure :fast @13:43:52.80 CS.seek 7.5ml @13:43:59.59 CSV.dial :waste @13:44:00.87 CS.reconfigure :default @13:44:00.92 CS.seek 0ml @13:44:21.09 Purging external Intake @13:44:21.20 CSV.dial :flush @13:44:21.66 CS.reconfigure :fast @13:44:21.70 CS.seek 10ml @13:44:46.37 delay 5 seconds @13:44:51.39 CSV.dial CBV @13:44:51.42
CBV.dial CSV @13:44:52.18 CTV.dial :intake @13:44:52.98 Intake.select :open @13:44:53.08 CS.seek 0ml @13:45:16.99 Intake.select :close @13:45:17.09 CTV.dial :air @13:45:17.90 Flushing upper manifold @13:45:18.01 CSV.dial :flush @13:45:18.75 CS.seek 5ml @13:45:31.65 delay 5 seconds @13:45:36.67 CSV.dial :air @13:45:37.18 CS.seek 7ml @13:45:42.87 CSV.dial CBV @13:45:42.90
CBV.dial CSV @13:45:43.95 CTV.dial :air @13:45:43.97 CS.seek 0ml @13:46:00.87 delay 7 seconds @13:46:07.89 CS.seek 3ml @13:46:15.90 CSV.dial :waste @13:46:16.36 CS.seek 0ml @13:46:24.25 CSV.dial :flush @13:46:24.69 CS.seek 3ml @13:46:32.71 delay 5 seconds @13:46:37.73 CSV.dial :air @13:46:38.24 CS.seek 5ml @13:46:43.87 CSV.dial :air @13:46:43.88 CS.seek 0ml @13:46:56.12 delay 7 seconds @13:47:03.14 CS.seek 3ml @13:47:11.12 CSV.dial :waste @13:47:11.86 CS.seek 0ml @13:47:19.59 Garaging FlushPuck in Collection Clamp @13:47:19.65 Elbow.reconfigure :tight @13:47:19.69 Elbow.seek CC @13:47:20.08 Elbow.reconfigure :loose @13:47:20.17 Forearm.seek CC @13:47:21.10 Hand.close @13:47:21.67 CC.seek :open @13:47:26.29 Forearm.seek :retract @13:47:27.23 Elbow.reconfigure :tight @13:47:27.28 Elbow.seek :garage @13:47:30.17 Elbow.reconfigure :loose @13:47:30.26 Forearm.reconfigure :tight @13:47:30.30 Forearm.seek :garage @13:47:31.25 Hand.open @13:47:32.32 Forearm.reconfigure :loose @13:47:32.43 Forearm.seek :retract @13:47:33.36 da sampling at most 100ml, wcr at most 100ml @13:47:33.39 Loading sh1 puck from tube 2 into Collection Clamp @13:47:33.41 DAfans.email "Started da in tube 2 at 13:47:33UTC27-Dec-21\n{1 => 8,\n 2 => 25,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@13:43:13, 18% flow, 15.8C, 35% humidity, 14.6psia, 13.637V, 0.345A, 0.327A avg, 4.70W",Subject: "da sampling at most 100ml, wcr at most 100ml" @13:47:33.65
SC.reconfigure :tight @13:47:33.69 SC.seek 2 @13:47:40.42 SC.configure :limp @13:47:40.65 Elbow.reconfigure :tight @13:47:40.69 Elbow.seek 2 @13:47:42.79 Forearm.reconfigure :tight @13:47:42.88 Forearm.seek 2 + 450 counts @13:47:44.01 SE.reconfigure :pick @13:47:44.11 SE.seek :beamBreak @13:47:45.72 SE.configure :default @13:47:46.04 Elbow.seek 2 @13:47:46.41 Forearm.seek 2 + 300 counts @13:47:46.64 Forearm.reconfigure :loose @13:47:46.79 Hand.close @13:47:47.47 SE.seek :down @13:47:47.94
Forearm.seek :retract @13:47:48.82 SE.stop @13:47:48.94
Elbow.seek CC @13:47:50.75 Elbow.reconfigure :loose @13:47:50.93 Forearm.reconfigure :tight @13:47:51.01 Forearm.seek CC @13:47:52.64 CC.seek :closed @13:47:57.36 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.17 @13:47:57.38 Hand.open @13:47:58.18 Forearm.reconfigure :loose @13:47:58.28 Forearm.seek :retract @13:47:59.36 collectSample 100ml w/bubble point of 24psi @13:47:59.38 Priming 7ml @13:47:59.40 Intake.select :close @13:47:59.42 Exhaust.select :close @13:47:59.44 SS.hold @13:47:59.87 Calibrating pressure and temperature sensors @13:47:59.93 CTV.dial :air @13:47:59.95 SSV.dial :spit,avoiding: :exhaust @13:47:59.99 SS.reconfigure :fast @13:48:00.07 SS.seek 5ml @13:48:02.99 SSV.dial CBV @13:48:03.05
CBV.dial SSV @13:48:04.46 delay 3.8 seconds @13:48:08.45 SSV.dial :spit @13:48:09.84 SS.seek 0ml @13:48:12.79 SSV.dial CBV @13:48:14.15 CTV.dial :intake @13:48:15.01 SSV.dial CBV @13:48:15.05
CBV.dial SSV @13:48:15.07 Allowing 40s for 7.0ml of remaining 7.0ml @13:48:15.09 Intake.select :open @13:48:17.40 Intake @14.5psia, vacuum from 9.0 to 12.0psi @13:48:17.44 SS.reconfigure :inhaling 2.20ml/s @13:48:17.50 SS.seek 7ml @13:48:23.68 Stroke completed -- waiting for pressure to equalize ... @13:48:23.72 SS.reconfigure :exhale @13:48:23.80 SS.hold @13:48:27.16 Intake.select :close @13:48:27.41 SSV.dial :exhaust @13:48:28.20 Exhaust.select :open @13:48:28.41 SS.seek 0ml @13:48:32.54 Exhaust.select :close @13:48:32.71 Sampling 100.0ml Intake.select :close @13:48:32.73 Exhaust.select :close @13:48:32.75 SS.reconfigure :default @13:48:32.82 SS.hold @13:48:33.23 CTV.dial :intake @13:48:33.25 SSV.dial CBV @13:48:33.32
CBV.dial SSV @13:48:34.17 Allowing 130s for 25.0ml of remaining 100.0ml @13:48:34.19 Intake.select :open @13:48:36.47 Intake @15.3psia, vacuum from 9.8 to 12.8psi @13:48:36.49 SS.reconfigure :inhaling 2.20ml/s @13:48:36.57 SS.seek 25ml @13:48:43.87 upsync @13:48:51.77
Stroke completed -- waiting for pressure to equalize ... @13:48:51.78 SS.reconfigure :exhale @13:48:51.82 SS.hold @13:48:56.13 Intake.select :close @13:48:56.26 SSV.dial :exhaust @13:48:57.04 Exhaust.select :open @13:48:57.15 SS.seek 0ml @13:49:05.85 Exhaust.select :close @13:49:05.95 SSV.dial CBV @13:49:05.97
CBV.dial SSV @13:49:06.74 Allowing 130s for 25.0ml of remaining 75.0ml @13:49:06.75 Intake.select :open @13:49:08.90 Intake @14.0psia, vacuum from 8.5 to 11.5psi @13:49:08.92 SS.reconfigure :inhaling 2.20ml/s @13:49:08.96 SS.seek 25ml @13:49:24.14 Stroke completed -- waiting for pressure to equalize ... @13:49:24.15 SS.reconfigure :exhale @13:49:24.19 SS.hold @13:49:28.93 Intake.select :close @13:49:29.07 SSV.dial :exhaust @13:49:29.85 Exhaust.select :open @13:49:29.95 SS.seek 0ml @13:49:38.59 Exhaust.select :close @13:49:38.70 SSV.dial CBV @13:49:38.72
CBV.dial SSV @13:49:39.49 Allowing 130s for 25.0ml of remaining 50.0ml @13:49:39.50 Intake.select :open @13:49:41.65 Intake @13.8psia, vacuum from 8.3 to 11.3psi @13:49:41.66 SS.reconfigure :inhaling 2.20ml/s @13:49:41.71 SS.seek 25ml @13:49:57.11 Stroke completed -- waiting for pressure to equalize ... @13:49:57.12 SS.reconfigure :exhale @13:49:57.16 SS.hold @13:50:02.68 Intake.select :close @13:50:02.82 SSV.dial :exhaust @13:50:03.60 Exhaust.select :open @13:50:03.70 SS.seek 0ml @13:50:12.67 Exhaust.select :close @13:50:12.78 SSV.dial CBV @13:50:12.80
CBV.dial SSV @13:50:13.58 Allowing 130s for 25.0ml of remaining 25.0ml @13:50:13.59 Intake.select :open @13:50:15.74 Intake @13.9psia, vacuum from 8.4 to 11.4psi @13:50:15.76 SS.reconfigure :inhaling 2.20ml/s @13:50:15.80 SS.seek 25ml @13:50:30.59 Stroke completed -- waiting for pressure to equalize ... @13:50:30.60 SS.reconfigure :exhale @13:50:30.64 SS.hold @13:50:36.16 Intake.select :close @13:50:36.30 SSV.dial :exhaust @13:50:37.06 Exhaust.select :open @13:50:37.17 SS.seek 0ml @13:50:45.94 Exhaust.select :close @13:50:46.04 Sampled 100.0ml @13:50:46.06 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @13:50:46.12 CSV.dial :waste @13:50:46.16 CS.seek 0ml @13:50:46.26 SSV.dial CBV,avoiding: :exhaust @13:50:46.29 CSV.dial :air @13:50:46.32
CBV.dial SSV @13:50:47.09 CS.seek 10ml @13:50:48.21
CTV.dial :air @13:50:49.01 delay 15 seconds @13:51:04.02 SSV.dial :spit,avoiding: :exhaust @13:51:05.35 CTV.dial CSV,avoiding: :intake @13:51:05.39
SS.seek 0ml @13:51:05.50 SSV.dial CBV,avoiding: :exhaust @13:51:11.70 CBV.dial between SSV and waste2 @13:51:12.08 CTV.dial CSV,avoiding: :air @13:51:12.10
CSV.dial CTV @13:51:12.67 CS.reconfigure :pressurize @13:51:12.71 CS.seek 0ml @13:51:31.15 BubblePoint: Intake @38.8psia, Outlet @14.6psia CBV.dial SSV @13:51:31.49 SS.reconfigure :default @13:51:31.54 SS.seek 10ml @13:51:37.17 delay 15 seconds @13:51:52.28 Evacuated: Intake @15.7psia, Outlet @9.9psia @13:51:52.30 SSV.dial :spit,avoiding: :exhaust @13:51:53.63 SS.reconfigure :exhale @13:51:53.67 SS.seek 0ml @13:51:59.03 SS.reconfigure :fast @13:51:59.07 SS.seek 5ml @13:52:01.94 SSV.dial CBV,avoiding: :exhaust @13:52:01.96
CBV.dial SSV @13:52:03.28 CTV.dial :air,avoiding: :intake @13:52:04.68 CSV.dial CTV @13:52:04.71
SS.seek 10ml @13:52:05.71 CSV.dial :air @13:52:06.15 CS.reconfigure :fast @13:52:06.19 CS.seek 0ml @13:52:07.17
SSV.dial :spit,avoiding: :exhaust @13:52:08.51 SS.reconfigure :exhale @13:52:08.55 SS.seek 0ml @13:52:13.48 SS.reconfigure :fast @13:52:13.52 SS.seek 5ml @13:52:14.33 CS.stop @13:52:16.43 SSV.dial CBV,avoiding: :exhaust @13:52:16.46
CBV.dial SSV @13:52:18.77 SSV.dial :spit,avoiding: :exhaust @13:52:20.10 SS.reconfigure :default @13:52:20.14 SS.seek 0ml @13:52:23.36 SS.stop @13:52:23.39 Evacuating Puck (bubblePt=24psi, evacVolume=10ml, delay 15 seconds) @13:52:23.40 Intake.select :close @13:52:23.47 CSV.dial :waste @13:52:23.58 SSV.dial CBV,avoiding: :exhaust @13:52:23.60
CBV.dial SSV @13:52:24.29 CS.seek 0ml @13:52:24.36 CSV.dial :air @13:52:24.94
CTV.dial :air @13:52:24.95 delay 15 seconds @13:52:25.09 CS.seek 10ml @13:52:39.97
SSV.dial :spit,avoiding: :exhaust @13:52:41.30 CTV.dial CSV,avoiding: :intake @13:52:41.36
SS.reconfigure :exhale @13:52:41.42 SS.seek 0ml @13:52:41.88 SSV.dial CBV,avoiding: :exhaust @13:52:49.57 CBV.dial between SSV and waste2 @13:52:49.97 CTV.dial CSV,avoiding: :air @13:52:49.99
CSV.dial CTV @13:52:50.45 CS.reconfigure :pressurize @13:52:50.49 CS.seek 0ml @13:53:11.86 BubblePoint: Intake @38.0psia, Outlet @13.7psia @13:53:11.87 CBV.dial SSV @13:53:12.19 SS.reconfigure :default @13:53:12.24 SS.seek 10ml @13:53:17.86 delay 15 seconds @13:53:32.97 Evacuated: Intake @17.4psia, Outlet @11.7psia @13:53:32.98 SSV.dial :spit,avoiding: :exhaust @13:53:34.32 SS.reconfigure :exhale @13:53:34.36 SS.seek 0ml @13:53:39.62 SS.reconfigure :fast @13:53:39.66 SS.seek 5ml @13:53:42.52 SSV.dial CBV,avoiding: :exhaust @13:53:42.54
CBV.dial SSV @13:53:43.88 CTV.dial :air,avoiding: :intake @13:53:45.27 CSV.dial CTV @13:53:45.30
SS.seek 10ml @13:53:46.30 CSV.dial :air @13:53:46.76 CS.reconfigure :fast @13:53:46.80 CS.seek 0ml @13:53:47.72
SSV.dial :spit,avoiding: :exhaust @13:53:49.07 SS.reconfigure :exhale @13:53:49.11 SS.seek 0ml @13:53:52.77 CS.stop @13:53:54.05
SS.reconfigure :fast @13:53:54.09 SS.seek 5ml @13:53:56.94 SSV.dial CBV,avoiding: :exhaust @13:53:56.99
CBV.dial SSV @13:53:59.29 SSV.dial :spit,avoiding: :exhaust @13:54:00.61 SS.reconfigure :default @13:54:00.65 SS.seek 0ml @13:54:03.92 SS.stop @13:54:03.96 Lysing: Adding 2.0ml AqMeOH @13:54:03.97 CBV.dial between CSV and waste1,avoiding: :DBV @13:54:05.46 CTV.dial CSV @13:54:06.81 CSV.dial CSR @13:54:07.58 Pulling 1ml of CSR.AqMeOH @13:54:07.59 CSR.select :AqMeOH @13:54:07.70 CS.reconfigure :default @13:54:07.74 CS.seek 1ml @13:54:11.11 delay 5 seconds @13:54:16.13 CSR.select :air @13:54:16.28 CS.seek 6ml @13:54:29.95 delay 2 seconds @13:54:31.97 CSV.dial :waste2 @13:54:32.42 CS.reconfigure :fast @13:54:32.46 CS.seek 0ml @13:54:47.11 CSV.dial CSR @13:54:47.61 Pulling 2ml of CSR.AqMeOH @13:54:47.62 CSR.select :AqMeOH @13:54:47.73 CS.reconfigure :default @13:54:47.77 CS.seek 2ml @13:54:53.74 delay 5 seconds @13:54:58.75 CSR.select :air @13:54:58.90 CS.seek 5.001ml @13:55:07.52 delay 2 seconds @13:55:09.53 CSV.dial CTV @13:55:09.96 CS.seek 1ml @13:55:21.21 Before lysing Collection Puck Heater at 16C/38.1psia @13:55:21.23 Heating Puck.from 2, :sh1 in Collection Clamp to 70C for 10:00 @13:55:21.25 CH.seek 70C,8:00 @13:57:27.32 CH.hold 10:00,70C @13:57:27.49 Begun lysing Collection Puck Heater at 68C/44.0psia @14:07:27.51 Ended lysing Collection Puck Heater at 70C/42.7psia @14:07:27.53 CS.seek 0ml @14:07:31.17 CTV.dial between spare and air,avoiding: :intake @14:07:32.24 CSV.dial :air @14:07:32.69 CS.seek 5ml @14:07:46.44 CSV.dial CTV @14:07:46.88 delay 2 seconds @14:07:48.89 CSV.dial :air @14:07:49.46 delay 2 seconds @14:07:51.47 CSV.dial CTV @14:07:51.91 delay 2 seconds @14:07:53.93 CSV.dial :air @14:07:54.49 delay 2 seconds @14:07:56.51 CS.seek 0ml @14:08:10.18 Puck @70.7psia after final lysis pressurization @14:08:10.19 Recovering Lysate into Collection syringe @14:08:10.20 CSV.dial CBV @14:08:11.24 CBV.dial CSV @14:08:11.61 CS.seek 4.999ml @14:08:25.35 delay 5 seconds @14:08:30.36 CTV.dial :air @14:08:30.91 CS.seek 7ml @14:08:36.78 delay 5 seconds @14:08:41.80 CSV.dial :air2 @14:08:42.24 CS.seek 10ml @14:08:50.79 CSV.dial between air and CTV,avoiding: CBV @14:08:51.96 Lysing Complete @14:08:52.03 Storing sh1 puck in Collection Clamp to tube 1 @14:08:52.18 SC.reconfigure :tight @14:08:52.22 SC.seek 1 @14:08:55.82 SC.configure :limp @14:08:56.01 Elbow.reconfigure :tight @14:08:56.05 Elbow.seek 1 @14:08:57.81 Forearm.reconfigure :tight @14:08:57.85 Forearm.seek 1 + 450 counts @14:08:58.84 SE.reconfigure :pick @14:08:58.88 SE.seek :beamBreak @14:09:01.57 SE.configure :default @14:09:01.71 Elbow.reconfigure :limp @14:09:01.75 Forearm.reconfigure :loose @14:09:01.79 Forearm.seek 1 @14:09:02.03 Elbow.hold @14:09:02.08 Elbow.reconfigure :tight @14:09:02.17 SE.seek 7.12 Pucks below Top Plate @14:09:02.80 Forearm.force -20 @14:09:02.83 Forearm.seek 1 + 300 counts @14:09:03.11 Forearm.reconfigure :tight @14:09:03.15 Forearm.seek 1 + 450 counts @14:09:03.33 SE.seek 8.12 Pucks below Top Plate @14:09:03.84 Forearm.reconfigure :loose @14:09:03.88 Forearm.seek :retract @14:09:04.80 Elbow.seek CC @14:09:06.50 Elbow.reconfigure :loose @14:09:06.60 Forearm.seek CC @14:09:07.57 Hand.close @14:09:08.15 CC.seek :open @14:09:12.86 Forearm.seek :retract @14:09:13.84 Elbow.reconfigure :tight @14:09:13.88 Elbow.seek 1 @14:09:15.65 Elbow.reconfigure :limp @14:09:15.69 Forearm.seek 1 @14:09:16.67 Elbow.hold @14:09:16.73 Elbow.reconfigure :tight @14:09:16.77 Hand.open @14:09:17.51 Forearm.force -20 @14:09:18.02 Forearm.seek 1 + 309 counts @14:09:18.31 Forearm.reconfigure :tight @14:09:18.36 Forearm.seek 1 + 450 counts @14:09:18.61 SE.seek :down @14:09:19.73
Forearm.reconfigure :loose @14:09:19.77 Forearm.seek :retract @14:09:20.78 SE.stop @14:09:20.82
Tranferring DA @14:09:20.84 Beginning samplePassC @14:09:20.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:09:21.02 Elbow.seek FlushPuck::Garage @14:09:22.85 Elbow.reconfigure :loose @14:09:22.93 Forearm.seek :garage @14:09:23.90 Hand.close @14:09:24.64 Forearm.seek :retract @14:09:25.61 Elbow.reconfigure :tight @14:09:25.65 Elbow.seek CC @14:09:28.57 Elbow.reconfigure :loose @14:09:28.66 Forearm.reconfigure :tight @14:09:28.70 Forearm.seek CC @14:09:30.01 CC.seek :closed @14:09:34.52 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.29 @14:09:34.59 Hand.open @14:09:35.21 Forearm.reconfigure :loose @14:09:35.25 Forearm.seek :retract @14:09:36.24 PSV.dial PSR @14:09:37.64 Pulling 1.5ml of PSR.flush @14:09:37.65 PSR.select :flush @14:09:37.77 PS.seek 1.5ml @14:09:53.95 delay 5 seconds @14:09:58.97 PSV.dial :waste1 @14:09:59.43 PS.seek 0ml @14:10:15.57 PSV.dial PSR @14:10:16.03 PSR.select :air @14:10:16.14 PS.seek 2.5ml @14:10:42.52 PRV.dial CBV,avoiding: :ab2 @14:10:42.54
CBV.dial PRV @14:10:44.26 PSV.dial :mixloop @14:10:45.30 CTV.dial :air,avoiding: :intake @14:10:45.31 PTV.dial :PRVmixing @14:10:46.63 PS.seek 0ml @14:11:12.92 PSV.dial :air @14:11:13.50 PS.seek 2.5ml @14:11:39.92 PSV.dial :mixloop @14:11:40.36 PS.seek 0ml @14:12:06.73 PSV.dial :air @14:12:07.33 PS.seek 2.5ml @14:12:33.82 PSV.dial :mixloop @14:12:34.27 PS.seek 0ml @14:13:00.61 Garaging FlushPuck in Collection Clamp @14:13:00.68 Elbow.reconfigure :tight @14:13:00.72 Elbow.seek CC @14:13:01.00 Elbow.reconfigure :loose @14:13:01.09 Forearm.seek CC @14:13:02.07 Hand.close @14:13:02.65 CC.seek :open @14:13:07.26 Forearm.seek :retract @14:13:08.23 Elbow.reconfigure :tight @14:13:08.27 Elbow.seek :garage @14:13:11.26 Elbow.reconfigure :loose @14:13:11.35 Forearm.reconfigure :tight @14:13:11.39 Forearm.seek :garage @14:13:12.43 Hand.open @14:13:13.08 Forearm.reconfigure :loose @14:13:13.12 Forearm.seek :retract @14:13:14.10 PSV.dial :mixloop @14:13:14.11 PTV.dial :PRVmixing @14:13:14.13 PRV.dial CBV @14:13:14.15
CBV.dial PRV @14:13:14.17 CTV.dial :air,avoiding: :intake @14:13:14.18 Loading lyfil puck from tube 2 into Collection Clamp @14:13:14.88 SC.reconfigure :tight @14:13:14.92 SC.seek 2 @14:13:18.47 SC.configure :limp @14:13:18.66 Elbow.reconfigure :tight @14:13:18.71 Elbow.seek 2 @14:13:20.64 Forearm.reconfigure :tight @14:13:20.68 Forearm.seek 2 + 450 counts @14:13:21.69 SE.reconfigure :pick @14:13:21.74 SE.seek :beamBreak @14:13:23.27 SE.configure :default @14:13:23.41 Elbow.seek 2 @14:13:23.59 Forearm.seek 2 + 300 counts @14:13:23.95 Forearm.reconfigure :loose @14:13:23.99 Hand.close @14:13:24.66 SE.seek :down @14:13:24.85
Forearm.seek :retract @14:13:25.79 Elbow.seek CC @14:13:25.88 SE.stop @14:13:27.41
Elbow.reconfigure :loose @14:13:27.50 Forearm.reconfigure :tight @14:13:27.54 Forearm.seek CC @14:13:28.80 CC.seek :closed @14:13:33.31 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.05 @14:13:33.32 Hand.open @14:13:33.96 Forearm.reconfigure :loose @14:13:34.00 Forearm.seek :retract @14:13:34.96 CTV.dial CSV,avoiding: :intake @14:13:36.34 CSV.dial CTV @14:13:36.66 PS.seek 2.5ml @14:14:03.10 CS.seek 4.5ml @14:14:18.06 delay 8 seconds @14:14:26.08 PSV.dial :air @14:14:26.54 delay 2 seconds @14:14:28.56 PSV.dial :waste2 @14:14:29.28 PS.seek 0ml @14:14:55.66 CBV.dial between PRV and waste1 @14:14:55.98 CSV.dial :air @14:14:56.44 CS.seek 10ml @14:15:11.31 CSV.dial CTV @14:15:11.75 CS.seek 0ml @14:15:38.09 CBV.dial :waste1 @14:15:38.39 delay 5 seconds @14:15:43.41 CTV.dial :air,avoiding: :intake @14:15:44.85 Storing lyfil puck in Collection Clamp to tube 1 @14:15:45.01 SC.reconfigure :tight @14:15:45.05 SC.seek 1 @14:15:48.63 SC.configure :limp @14:15:48.82 Elbow.reconfigure :tight @14:15:48.87 Elbow.seek 1 @14:15:50.63 Forearm.reconfigure :tight @14:15:50.68 Forearm.seek 1 + 450 counts @14:15:51.71 SE.reconfigure :pick @14:15:51.76 SE.seek :beamBreak @14:15:54.30 SE.configure :default @14:15:54.44 Elbow.reconfigure :limp @14:15:54.49 Forearm.reconfigure :loose @14:15:54.53 Forearm.seek 1 @14:15:54.74 Elbow.hold @14:15:54.80 Elbow.reconfigure :tight @14:15:54.88 SE.seek 8.13 Pucks below Top Plate @14:15:55.34 Forearm.force -20 @14:15:55.38 Forearm.seek 1 + 314 counts @14:15:56.12 Forearm.reconfigure :tight @14:15:56.16 Forearm.seek 1 + 450 counts @14:15:56.34 SE.seek 9.13 Pucks below Top Plate @14:15:56.96 Forearm.reconfigure :loose @14:15:57.01 Forearm.seek :retract @14:15:57.95 Elbow.seek CC @14:15:59.66 Elbow.reconfigure :loose @14:15:59.75 Forearm.seek CC @14:16:00.71 Hand.close @14:16:01.28 CC.seek :open @14:16:05.99 Forearm.seek :retract @14:16:06.93 Elbow.reconfigure :tight @14:16:06.97 Elbow.seek 1 @14:16:08.75 Elbow.reconfigure :limp @14:16:08.80 Forearm.seek 1 @14:16:09.77 Elbow.hold @14:16:09.83 Elbow.reconfigure :tight @14:16:09.87 Hand.open @14:16:10.63 Forearm.force -20 @14:16:10.67 Forearm.seek 1 + 301 counts @14:16:10.96 Forearm.reconfigure :tight @14:16:11.00 Forearm.seek 1 + 450 counts @14:16:11.24 SE.seek :down @14:16:12.37
Forearm.reconfigure :loose @14:16:12.41 Forearm.seek :retract @14:16:13.33 SE.stop @14:16:13.38
PSV.dial :air @14:16:14.12 PS.seek 2.5ml @14:16:40.44 delay 1 second @14:16:41.46 PSV.dial :mixloop @14:16:41.90 delay 1 second @14:16:42.91 PSV.dial :air @14:16:43.37 delay 1 second @14:16:44.38 PSV.dial :mixloop @14:16:44.84 delay 1 second @14:16:45.85 PSV.dial :air @14:16:46.30 delay 1 second @14:16:47.32 PS.seek 0ml @14:17:13.66 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @14:17:13.83 Elbow.seek FlushPuck::Garage @14:17:15.68 Elbow.reconfigure :loose @14:17:15.77 Forearm.seek :garage @14:17:16.71 Hand.close @14:17:17.38 Forearm.seek :retract @14:17:18.35 Elbow.reconfigure :tight @14:17:18.39 Elbow.seek PC @14:17:19.65 Elbow.reconfigure :loose @14:17:19.74 Forearm.reconfigure :tight @14:17:19.78 Forearm.seek PC @14:17:20.94 PC.seek :closed @14:17:25.57 Processing Clamp onSpring/offSpring for 15/26 samples, on/off torque 1.54 @14:17:25.63 Hand.open @14:17:26.33 Forearm.reconfigure :loose @14:17:26.37 Forearm.seek :retract @14:17:27.30 PRV.dial :air @14:17:27.74 PSV.dial :mixloop @14:17:28.27 PS.seek 0.3ml @14:17:32.19 PSV.dial PSR @14:17:33.29 Pulling 0.4ml of PSR.air @14:17:33.30 PSR.select :air @14:17:33.32 PS.seek 0.7ml @14:17:38.19 delay 3 seconds @14:17:41.21 PSR.select :air @14:17:41.23 PS.seek 2.5ml @14:18:00.91 delay 3 seconds @14:18:03.93 PSV.dial :mixloop @14:18:04.97 PTV.dial :mixing @14:18:05.72 PBV.dial :waste2 @14:18:06.67 PS.seek 0ml @14:18:33.04 Garaging FlushPuck in Processing Clamp @14:18:33.11 Elbow.reconfigure :tight @14:18:33.15 Elbow.seek PC @14:18:33.26 Elbow.reconfigure :loose @14:18:33.35 Forearm.seek PC @14:18:34.66 Hand.close @14:18:35.27 PC.seek :open @14:18:39.99 Forearm.seek :retract @14:18:40.91 Elbow.reconfigure :tight @14:18:40.95 Elbow.seek :garage @14:18:42.31 Elbow.reconfigure :loose @14:18:42.40 Forearm.reconfigure :tight @14:18:42.44 Forearm.seek :garage @14:18:43.38 Hand.open @14:18:44.08 Forearm.reconfigure :loose @14:18:44.12 Forearm.seek :retract @14:18:45.08 Loading da puck from tube 2 into Processing Clamp @14:18:45.29 SC.reconfigure :tight @14:18:45.33 SC.seek 2 @14:18:48.89 SC.configure :limp @14:18:49.07 Elbow.reconfigure :tight @14:18:49.11 Elbow.seek 2 @14:18:50.98 Forearm.reconfigure :tight @14:18:51.02 Forearm.seek 2 + 450 counts @14:18:52.06 SE.reconfigure :pick @14:18:52.11 SE.seek :beamBreak @14:18:53.81 SE.configure :default @14:18:53.95 Elbow.seek 2 @14:18:54.12 Forearm.seek 2 + 300 counts @14:18:54.28 Forearm.reconfigure :loose @14:18:54.33 Hand.close @14:18:54.91 SE.seek :down @14:18:55.10
Forearm.seek :retract @14:18:56.08 Elbow.seek PC @14:18:56.23 SE.stop @14:18:58.64
Elbow.reconfigure :loose @14:18:58.72 Forearm.reconfigure :tight @14:18:58.76 Forearm.seek PC @14:19:00.02 PC.seek :closed @14:19:04.67 Processing Clamp onSpring/offSpring for 16/26 samples, on/off torque 1.58 @14:19:04.68 Hand.open @14:19:05.33 Forearm.reconfigure :loose @14:19:05.37 Forearm.seek :retract @14:19:06.31 Heating Puck.from 2, :da in Processing Clamp to 28C for 2:00:00 @14:19:06.33 PH.seek 28C,5:00 @14:19:59.90 PH.hold 2:00:00,28C @14:19:59.94 delay 1:30 @14:21:30.47 PSR.select :air @14:21:30.49 PSV.dial PSR @14:21:31.60 Pulling 0.5ml of PSR.wash @14:21:31.61 PSR.select :wash @14:21:31.73 PS.seek 0.5ml @14:21:37.66 delay 5 seconds @14:21:42.68 PSR.select :air @14:21:42.79 PS.seek 2ml @14:21:58.94 PTV.dial :puck @14:21:59.66 PSV.dial PTV @14:22:00.42 PBV.dial :waste1 @14:22:01.83 PS.seek 1.5ml @14:22:07.72 delay 3 seconds @14:22:10.74 PBV.dial between PSV and waste1 @14:22:11.10 PS.seek 0ml @14:22:27.25 delay 5:00 @14:27:27.27 recoverPSRreagent08 @14:27:27.28 PTV.dial :puck @14:27:27.29 PBV.dial :waste1 @14:27:27.65 delay 30 seconds @14:27:57.68 PBV.dial between PSV and waste1 @14:27:58.04 PSR.select :air @14:27:58.06 PSV.dial PSR @14:27:58.83 PS.reconfigure :fast @14:27:58.87 PS.seek 2.5ml @14:28:23.05 PSV.dial PTV @14:28:23.82 delay 5 seconds @14:28:28.84 PSV.dial PSR @14:28:29.61 delay 5 seconds @14:28:34.62 PSV.dial PTV @14:28:35.39 delay 5 seconds @14:28:40.40 PSV.dial :air @14:28:41.17 PS.seek 0ml @14:29:04.68 PTV.dial :PRVmixing,via: PRV @14:29:06.01 PSV.dial :mixloop @14:29:06.46 PRV.dial :ab1 @14:29:07.20 PS.reconfigure :slow @14:29:07.24 PS.seek 0.15ml @14:29:10.87 delay 2 seconds @14:29:12.88 CBV.dial PRV @14:29:12.91
PRV.dial CBV @14:29:13.47 PS.seek 0.35ml @14:29:18.06 delay 2 seconds @14:29:20.07 CBV.dial between PRV and PSV @14:29:20.38 PRV.dial :air @14:29:20.83 PS.seek 0.9ml @14:29:32.52 PRV.dial :waste2 @14:29:33.27 PS.seek 0.5ml @14:29:41.99 addPMLReagent08 @14:29:42.00 PSV.dial :air @14:29:42.47 PS.reconfigure :default @14:29:42.51 PS.seek 2ml @14:29:58.71 PSV.dial :PML @14:29:59.16 PTV.dial :mixing @14:29:59.93 PBV.dial :waste1 @14:30:00.35 PS.seek 1.85ml @14:30:02.68 PBV.dial between PSV and waste1 @14:30:03.06 PS.seek 0ml @14:30:22.71 delay 2 seconds @14:30:24.73 Beginning samplePassC @14:30:24.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:30:24.91 Elbow.reconfigure :tight @14:30:24.95 Elbow.seek FlushPuck::Garage @14:30:26.28 Elbow.reconfigure :loose @14:30:26.37 Forearm.seek :garage @14:30:27.34 Hand.close @14:30:28.00 Forearm.seek :retract @14:30:28.97 Elbow.reconfigure :tight @14:30:29.01 Elbow.seek CC @14:30:31.86 Elbow.reconfigure :loose @14:30:31.95 Forearm.reconfigure :tight @14:30:31.99 Forearm.seek CC @14:30:33.16 CC.seek :closed @14:30:37.71 Collection Clamp onSpring/offSpring for 14/26 samples, on/off torque 2.36 @14:30:37.79 Hand.open @14:30:38.89 Forearm.reconfigure :loose @14:30:38.94 Forearm.seek :retract @14:30:39.88 PSV.dial PSR @14:30:41.14 Pulling 1.5ml of PSR.flush @14:30:41.15 PSR.select :flush @14:30:41.26 PS.seek 1.5ml @14:30:57.49 delay 5 seconds @14:31:02.51 PSV.dial :waste1 @14:31:03.00 PS.seek 0ml @14:31:19.21 PSV.dial PSR @14:31:19.66 PSR.select :air @14:31:19.77 PS.seek 2.5ml @14:31:46.18 PRV.dial CBV,avoiding: :ab2 @14:31:46.21
CBV.dial PRV @14:31:47.83 PSV.dial :mixloop @14:31:48.88 CTV.dial :air,avoiding: :intake @14:31:48.89 PTV.dial :PRVmixing @14:31:49.65 PS.seek 0ml @14:32:16.05 PSV.dial :air @14:32:16.51 PS.seek 2.5ml @14:32:42.92 PSV.dial :mixloop @14:32:43.40 PS.seek 0ml @14:33:09.77 PSV.dial :air @14:33:10.35 PS.seek 2.5ml @14:33:36.77 PSV.dial :mixloop @14:33:37.21 PS.seek 0ml @14:34:03.52 Garaging FlushPuck in Collection Clamp @14:34:03.59 Elbow.reconfigure :tight @14:34:03.63 Elbow.seek CC @14:34:03.94 Elbow.reconfigure :loose @14:34:04.03 Forearm.seek CC @14:34:05.04 Hand.close @14:34:05.64 CC.seek :open @14:34:10.27 Forearm.seek :retract @14:34:11.32 Elbow.reconfigure :tight @14:34:11.36 Elbow.seek :garage @14:34:14.28 Elbow.reconfigure :loose @14:34:14.37 Forearm.reconfigure :tight @14:34:14.41 Forearm.seek :garage @14:34:15.41 Hand.open @14:34:16.07 Forearm.reconfigure :loose @14:34:16.11 Forearm.seek :retract @14:34:17.07 delayUntil "14:38:24.72" @14:34:17.10 Whole Cell Archive sampling 100ml @14:34:17.12 Loading wcr puck from tube 2 into Collection Clamp @14:34:17.34 SC.reconfigure :tight @14:34:17.38 SC.seek 2 @14:34:17.47 SC.configure :limp @14:34:17.67 Elbow.reconfigure :tight @14:34:17.72 Elbow.seek 2 @14:34:19.70 Forearm.reconfigure :tight @14:34:19.74 Forearm.seek 2 + 450 counts @14:34:20.80 SE.reconfigure :pick @14:34:20.85 SE.seek :beamBreak @14:34:22.61 SE.configure :default @14:34:22.76 Elbow.seek 2 @14:34:22.93 Forearm.seek 2 + 300 counts @14:34:23.10 Forearm.reconfigure :loose @14:34:23.15 Hand.close @14:34:23.77 SE.seek :down @14:34:23.97 Forearm.seek :retract @14:34:24.89 Elbow.seek CC @14:34:25.13 SE.stop @14:34:26.50 Elbow.reconfigure :loose @14:34:26.59 Forearm.reconfigure :tight @14:34:26.64 Forearm.seek CC @14:34:27.86 CC.seek :closed @14:34:32.94 Collection Clamp onSpring/offSpring for 14/27 samples, on/off torque 2.19 @14:34:32.95 Hand.open @14:34:33.57 Forearm.reconfigure :loose @14:34:33.61 Forearm.seek :retract @14:34:34.56 collectSample 100ml w/bubble point of 35psi @14:34:34.57 Priming 7ml @14:34:34.58 Intake.select :close @14:34:34.59 Exhaust.select :close @14:34:34.60 SS.hold @14:34:34.79 Calibrating pressure sensors @14:34:34.80 CTV.dial :air @14:34:34.82 SSV.dial :spit,avoiding: :exhaust @14:34:34.84 SS.reconfigure :fast @14:34:34.88 SS.seek 5ml @14:34:37.59 SSV.dial CBV @14:34:37.62 CBV.dial SSV @14:34:38.98 delay 3.8 seconds @14:34:42.90 SSV.dial :spit @14:34:44.26 SS.seek 0ml @14:34:47.37 SSV.dial CBV @14:34:48.70 CTV.dial :intake @14:34:49.48 SSV.dial CBV @14:34:49.51 CBV.dial SSV @14:34:49.52 Allowing 40s for 7.0ml of remaining 7.0ml @14:34:49.53 Intake.select :open @14:34:51.69 Intake @14.2psia, vacuum from 8.7 to 11.7psi @14:34:51.71 SS.reconfigure :inhaling 2.20ml/s @14:34:51.75 SS.seek 7ml @14:34:57.40 Stroke completed -- waiting for pressure to equalize ... @14:34:57.41 SS.reconfigure :exhale @14:34:57.46 SS.hold @14:35:28.04 Intake.select :close @14:35:28.18 SSV.dial :exhaust @14:35:28.95 Exhaust.select :open @14:35:29.06 SS.seek 0ml @14:35:32.98 Exhaust.select :close @14:35:33.09 Sampling 100.0ml @14:35:33.10 Intake.select :close @14:35:33.11 Exhaust.select :close @14:35:33.12 SS.reconfigure :default @14:35:33.15 SS.hold @14:35:33.34 CTV.dial :intake @14:35:33.36 SSV.dial CBV @14:35:33.40 CBV.dial SSV @14:35:34.15 Allowing 130s for 25.0ml of remaining 100.0ml @14:35:34.17 Intake.select :open @14:35:36.32 Intake @15.8psia, vacuum from 10.0 to 13.0psi @14:35:36.34 SS.reconfigure :inhaling 2.20ml/s @14:35:36.38 SS.seek 25ml @14:35:42.60 Vacuum too high -- waiting up to 40 seconds @14:35:42.61 SS.reconfigure :exhale @14:35:42.65 SS.hold @14:35:47.82 Intake @16.1psia, vacuum from 10.0 to 13.0psi @14:35:48.31 SS.reconfigure :inhaling 1.98ml/s @14:35:48.37 SS.seek 25ml @14:35:52.44 Vacuum too high -- waiting up to 40 seconds @14:35:52.45 SS.reconfigure :exhale @14:35:52.50 SS.hold @14:35:56.92 Intake @15.7psia, vacuum from 10.0 to 13.0psi @14:35:56.94 SS.reconfigure :inhaling 1.79ml/s @14:35:56.99 SS.seek 25ml @14:36:02.41 Vacuum too high -- waiting up to 40 seconds @14:36:02.42 SS.reconfigure :exhale @14:36:02.47 SS.hold @14:36:08.30 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:36:08.32 SS.reconfigure :inhaling 1.61ml/s @14:36:08.36 SS.seek 25ml @14:36:12.57 Stroke completed -- waiting for pressure to equalize ... @14:36:12.58 SS.reconfigure :exhale @14:36:12.63 SS.hold @14:36:20.18 Intake.select :close @14:36:20.32 SSV.dial :exhaust @14:36:21.10 Exhaust.select :open @14:36:21.22 SS.seek 0ml @14:36:30.26 Exhaust.select :close @14:36:30.37 SSV.dial CBV @14:36:30.39 CBV.dial SSV @14:36:31.15 Allowing 130s for 25.0ml of remaining 75.0ml @14:36:31.17 Intake.select :open @14:36:33.32 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:36:33.34 SS.reconfigure :inhaling 1.61ml/s @14:36:33.38 SS.seek 25ml @14:36:44.88 Vacuum too high -- waiting up to 40 seconds @14:36:44.89 SS.reconfigure :exhale @14:36:44.93 SS.hold @14:36:50.72 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:36:50.74 SS.reconfigure :inhaling 1.45ml/s @14:36:50.78 SS.seek 25ml @14:36:59.56 Stroke completed -- waiting for pressure to equalize ... @14:36:59.57 SS.reconfigure :exhale @14:36:59.61 SS.hold @14:37:09.36 Intake.select :close @14:37:09.50 SSV.dial :exhaust @14:37:10.28 Exhaust.select :open @14:37:10.39 SS.seek 0ml @14:37:19.56 Exhaust.select :close @14:37:19.66 SSV.dial CBV @14:37:19.69 CBV.dial SSV @14:37:20.47 Allowing 130s for 25.0ml of remaining 50.0ml @14:37:20.48 Intake.select :open @14:37:22.64 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:37:22.65 SS.reconfigure :inhaling 1.45ml/s @14:37:22.70 SS.seek 25ml @14:37:36.59 Vacuum too high -- waiting up to 40 seconds @14:37:36.61 SS.reconfigure :exhale @14:37:36.65 SS.hold @14:37:43.24 Intake @15.3psia, vacuum from 9.8 to 12.8psi @14:37:43.26 SS.reconfigure :inhaling 1.30ml/s @14:37:43.30 SS.seek 25ml @14:37:51.65 Stroke completed -- waiting for pressure to equalize ... @14:37:51.66 SS.reconfigure :exhale @14:37:51.70 SS.hold @14:38:00.17 Intake.select :close @14:38:00.31 SSV.dial :exhaust @14:38:01.09 Exhaust.select :open @14:38:01.20 SS.seek 0ml @14:38:09.91 Exhaust.select :close @14:38:10.02 SSV.dial CBV @14:38:10.06 CBV.dial SSV @14:38:10.81 Allowing 130s for 25.0ml of remaining 25.0ml @14:38:10.82 Intake.select :open @14:38:12.97 Intake @15.5psia, vacuum from 10.0 to 13.0psi @14:38:12.99 SS.reconfigure :inhaling 1.30ml/s @14:38:13.03 SS.seek 25ml @14:38:24.73 recoverPRVreagent08 @14:38:24.74 PTV.dial :mixing @14:38:25.51 PBV.dial :waste1 @14:38:25.89 delay 30 seconds @14:38:31.63 Vacuum too high -- waiting up to 40 seconds @14:38:31.64 SS.reconfigure :exhale @14:38:31.68 SS.hold @14:38:39.17 Intake @14.8psia, vacuum from 9.3 to 12.3psi @14:38:39.18 SS.reconfigure :inhaling 1.17ml/s @14:38:39.23 SS.seek 25ml @14:38:43.69 Stroke completed -- waiting for pressure to equalize ... @14:38:43.71 SS.reconfigure :exhale @14:38:43.75 SS.hold @14:38:50.28 Intake.select :close @14:38:50.42 SSV.dial :exhaust @14:38:51.20 Exhaust.select :open @14:38:51.31 SS.seek 0ml @14:38:55.91 PBV.dial between PSV and waste1 @14:38:56.30 PSV.dial :air @14:38:56.77 PS.reconfigure :fast @14:38:57.30 PS.seek 2.5ml @14:39:00.01 Exhaust.select :close @14:39:00.11 Sampled 100.0ml @14:39:00.13 Drying puck before fixing for 20:00 @14:39:00.14 CBV.dial :waste2b @14:39:00.90 delay 1:00 @14:39:21.61 PSV.dial :mixloop @14:39:22.09 delay 2 seconds @14:39:24.11 PSV.dial :air @14:39:24.72 delay 2 seconds @14:39:26.73 PSV.dial :mixloop @14:39:27.19 delay 2 seconds @14:39:29.20 PSV.dial :air @14:39:29.68 PS.seek 0ml @14:39:53.36 washCycleDA #1 of 2 @14:39:53.37 PSR.select :air @14:39:53.38 PSV.dial PSR @14:39:54.77 Pulling 0.5ml of PSR.wash @14:39:54.78 PSR.select :wash @14:39:54.89 PS.reconfigure :default @14:39:54.93 PS.seek 0.5ml @14:40:00.89 delay 5 seconds @14:40:00.92 CSV.dial :air @14:40:01.37 CS.reconfigure :fast @14:40:01.42 CS.seek 10ml @14:40:05.90 PSR.select :air @14:40:06.02 PS.seek 2ml @14:40:22.20 PTV.dial :puck @14:40:22.93 PSV.dial PTV @14:40:23.81 PBV.dial :waste1 @14:40:24.23 PS.seek 1.5ml @14:40:26.04 Pumping wcr puck from tube 2 up to 35psi... @14:40:26.05 CBV.dial SSV @14:40:27.37 CBV.dial between CSV and waste2b @14:40:28.33 CSV.dial between air and CTV @14:40:28.66 CS.seek 7.322ml @14:40:30.17 delay 3 seconds @14:40:33.18 PBV.dial between PSV and waste1 @14:40:33.58 PS.seek 0ml @14:40:35.70 CTV.dial CSV,avoiding: :air @14:40:35.73 CSV.dial CTV @14:40:36.54 CS.reconfigure :pressurize @14:40:36.59 CS.seek 0ml @14:40:49.76 delay 2:00 @14:40:51.03 CBV.dial :waste2b @14:40:51.35 CS.reconfigure :default @14:40:51.40 CS.seek 0ml @14:40:56.85 delay 1:00 @14:41:56.88 CTV.dial :air,avoiding: :intake @14:41:58.26 Fix #1 of 2 (with RNAlater) for 10:00 @14:41:58.27 CSV.dial CSR @14:41:58.76 Pulling 2ml of CSR.RNAlater @14:41:58.77 CSR.select :RNAlater @14:41:58.88 CS.seek 2ml @14:42:04.88 delay 5 seconds @14:42:09.90 CSR.select :air @14:42:10.01 CS.reconfigure :fast @14:42:10.05 CS.seek 10ml @14:42:29.88 Pumping wcr puck from tube 2 up to 35psi... @14:42:29.89 CBV.dial SSV @14:42:31.21 CBV.dial between CSV and waste2b @14:42:32.18 CSV.dial between air and CTV @14:42:32.78 CS.seek 7.283ml @14:42:39.82 CTV.dial CSV,avoiding: :air @14:42:39.85 CSV.dial CTV @14:42:41.27 CS.reconfigure :pressurize @14:42:41.32 CS.seek 0ml @14:42:49.79 recoverPSRreagent08 @14:42:49.80 PTV.dial :puck @14:42:49.81 PBV.dial :waste1 @14:42:50.17 delay 30 seconds @14:42:56.99 delay 10:00 @14:43:20.18 PBV.dial between PSV and waste1 @14:43:20.56 PSR.select :air @14:43:20.57 PSV.dial PSR @14:43:21.33 PS.reconfigure :fast @14:43:21.38 PS.seek 2.5ml @14:43:45.54 PSV.dial PTV @14:43:46.41 delay 5 seconds @14:43:51.43 PSV.dial PSR @14:43:52.18 delay 5 seconds @14:43:57.20 PSV.dial PTV @14:43:57.96 delay 5 seconds @14:44:02.98 PSV.dial :air @14:44:04.21 PS.seek 0ml @14:44:27.88 washCycleDA #2 of 2 @14:44:27.89 PSR.select :air @14:44:27.91 PSV.dial PSR @14:44:29.29 Pulling 0.5ml of PSR.wash @14:44:29.30 PSR.select :wash @14:44:29.42 PS.reconfigure :default @14:44:29.46 PS.seek 0.5ml @14:44:35.45 delay 5 seconds @14:44:40.47 PSR.select :air @14:44:40.58 PS.seek 2ml @14:44:56.75 PTV.dial :puck @14:44:56.77 PSV.dial PTV @14:44:57.51 PBV.dial :waste1 @14:44:57.94 PS.seek 1.5ml @14:45:03.94 delay 3 seconds @14:45:06.96 PBV.dial between PSV and waste1 @14:45:07.34 PS.seek 0ml @14:45:23.51 delay 2:00 @14:47:23.53 recoverPSRreagent08 @14:47:23.54 PTV.dial :puck @14:47:23.55 PBV.dial :waste1 @14:47:23.91 delay 30 seconds @14:47:53.93 PBV.dial between PSV and waste1 @14:47:54.30 PSR.select :air @14:47:54.32 PSV.dial PSR @14:47:55.21 PS.reconfigure :fast @14:47:55.25 PS.seek 2.5ml @14:48:19.28 PSV.dial PTV @14:48:20.02 delay 5 seconds @14:48:25.04 PSV.dial PSR @14:48:25.80 delay 5 seconds @14:48:30.82 PSV.dial PTV @14:48:31.71 delay 5 seconds @14:48:36.72 PSV.dial :air @14:48:37.51 PS.seek 0ml @14:49:01.19 getPMLReagent08 @14:49:01.20 PRV.dial :air @14:49:01.66 delay 5 seconds @14:49:06.68 PTV.dial :PRVmixing @14:49:08.00 delay 5 seconds @14:49:13.02 PRV.dial :ab2 @14:49:13.48 PTV.dial :PRVmixing @14:49:13.50 PSV.dial :mixloop @14:49:13.96 PS.reconfigure :default @14:49:14.00 PS.seek 0.3ml @14:49:17.88 delay 2 seconds @14:49:19.89 PRV.dial :air @14:49:20.40 PS.seek 0.55ml @14:49:23.75 addPMLReagent08 @14:49:23.76 PSV.dial :air @14:49:24.23 PS.seek 2ml @14:49:39.87 PSV.dial :PML @14:49:40.33 PTV.dial :mixing @14:49:41.08 PBV.dial :waste1 @14:49:41.50 PS.seek 1.85ml @14:49:43.84 PBV.dial between PSV and waste1 @14:49:44.23 PS.seek 0ml @14:50:03.91 delay 2 seconds @14:50:05.92 delay 8:00 @14:52:57.02 CBV.dial :waste2b @14:52:57.34 CS.reconfigure :default @14:52:57.39 CS.seek 0ml @14:53:01.56 delay 1:00 @14:54:01.58 CTV.dial :air,avoiding: :intake @14:54:02.97 Fix #2 of 2 (with RNAlater) for 10:00 @14:54:02.98 CSV.dial CSR @14:54:03.61 Pulling 2ml of CSR.RNAlater @14:54:03.62 CSR.select :RNAlater @14:54:03.73 CS.seek 2ml @14:54:09.90 delay 5 seconds @14:54:14.92 CSR.select :air @14:54:15.03 CS.reconfigure :fast @14:54:15.08 CS.seek 10ml @14:54:35.12 Pumping wcr puck from tube 2 up to 35psi... @14:54:35.13 CBV.dial SSV @14:54:36.44 CBV.dial between CSV and waste2b @14:54:37.41 CSV.dial between air and CTV @14:54:38.00 CS.seek 7.227ml @14:54:45.18 CTV.dial CSV,avoiding: :air @14:54:45.21 CSV.dial CTV @14:54:46.64 CS.reconfigure :pressurize @14:54:46.69 CS.seek 0ml @14:55:01.33 delay 10:00 @14:58:05.94 recoverPRVreagent08 @14:58:05.95 PTV.dial :mixing @14:58:05.96 PBV.dial :waste1 @14:58:06.34 delay 30 seconds @14:58:36.36 PBV.dial between PSV and waste1 @14:58:36.73 PSV.dial :air @14:58:37.34 PS.reconfigure :fast @14:58:37.38 PS.seek 2.5ml @14:59:01.93 PSV.dial :mixloop @14:59:02.37 delay 2 seconds @14:59:04.38 PSV.dial :air @14:59:04.98 delay 2 seconds @14:59:07.00 PSV.dial :mixloop @14:59:07.44 delay 2 seconds @14:59:09.46 PSV.dial :air @14:59:10.07 PS.seek 0ml @14:59:33.83 washCycleDA #1 of 3 @14:59:33.84 PSR.select :air @14:59:33.86 PSV.dial PSR @14:59:35.25 Pulling 0.5ml of PSR.wash @14:59:35.26 PSR.select :wash @14:59:35.37 PS.reconfigure :default @14:59:35.41 PS.seek 0.5ml @14:59:41.52 delay 5 seconds @14:59:46.54 PSR.select :air @14:59:46.65 PS.seek 2ml @15:00:02.77 PTV.dial :puck @15:00:03.50 PSV.dial PTV @15:00:04.40 PBV.dial :waste1 @15:00:04.83 PS.seek 1.5ml @15:00:10.74 delay 3 seconds @15:00:13.76 PBV.dial between PSV and waste1 @15:00:14.14 PS.seek 0ml @15:00:30.37 delay 2:00 @15:02:30.38 recoverPSRreagent08 @15:02:30.39 PTV.dial :puck @15:02:30.40 PBV.dial :waste1 @15:02:30.77 delay 30 seconds @15:03:00.79 PBV.dial between PSV and waste1 @15:03:01.16 PSR.select :air @15:03:01.18 PSV.dial PSR @15:03:01.93 PS.reconfigure :fast @15:03:01.97 PS.seek 2.5ml @15:03:26.17 PSV.dial PTV @15:03:27.06 delay 5 seconds @15:03:32.08 PSV.dial PSR @15:03:32.83 delay 5 seconds @15:03:37.85 PSV.dial PTV @15:03:38.61 delay 5 seconds @15:03:43.63 PSV.dial :air @15:03:44.52 PS.seek 0ml @15:04:08.27 washCycleDA #2 of 3 @15:04:08.28 PSR.select :air @15:04:08.29 PSV.dial PSR @15:04:09.72 Pulling 0.5ml of PSR.wash @15:04:09.73 PSR.select :wash @15:04:09.84 PS.reconfigure :default @15:04:09.88 PS.seek 0.5ml @15:04:16.02 delay 5 seconds @15:04:21.04 PSR.select :air @15:04:21.15 PS.seek 2ml @15:04:37.33 PTV.dial :puck @15:04:37.34 PSV.dial PTV @15:04:38.09 PBV.dial :waste1 @15:04:38.51 PS.seek 1.5ml @15:04:44.45 delay 3 seconds @15:04:47.47 PBV.dial between PSV and waste1 @15:04:47.85 PS.seek 0ml @15:05:01.34 CBV.dial :waste2b @15:05:01.67 CS.reconfigure :default @15:05:01.72 CS.seek 0ml @15:05:04.02 delay 2:00 @15:05:06.71 delay 1:00 @15:06:06.73 CTV.dial :air,avoiding: :intake @15:07:04.04 recoverPSRreagent08 @15:07:04.05 PTV.dial :puck @15:07:04.06 PBV.dial :waste1 @15:07:04.44 delay 30 seconds @15:07:34.46 PBV.dial between PSV and waste1 @15:07:34.83 PSR.select :air @15:07:34.84 PSV.dial PSR @15:07:35.61 PS.reconfigure :fast @15:07:35.66 PS.seek 2.5ml @15:07:59.85 PSV.dial PTV @15:08:00.73 delay 5 seconds @15:08:05.75 PSV.dial PSR @15:08:06.50 delay 5 seconds @15:08:11.52 PSV.dial PTV @15:08:12.28 delay 5 seconds @15:08:17.30 PSV.dial :air @15:08:18.19 PS.seek 0ml @15:08:41.64 washCycleDA #3 of 3 @15:08:41.65 PSR.select :air @15:08:41.66 PSV.dial PSR @15:08:43.06 Pulling 0.5ml of PSR.wash @15:08:43.07 PSR.select :wash @15:08:43.18 PS.reconfigure :default @15:08:43.22 PS.seek 0.5ml @15:08:49.14 delay 5 seconds @15:08:54.16 PSR.select :air @15:08:54.27 PS.seek 2ml @15:09:10.48 PTV.dial :puck @15:09:10.50 PSV.dial PTV @15:09:11.26 PBV.dial :waste1 @15:09:11.68 PS.seek 1.5ml @15:09:17.57 delay 3 seconds @15:09:20.59 PBV.dial between PSV and waste1 @15:09:20.97 PS.seek 0ml @15:09:37.20 delay 2:00 @15:11:37.23 recoverPSRreagent08 PTV.dial :puck @15:11:37.24 PBV.dial :waste1 @15:11:37.60 delay 30 seconds @15:12:07.63 PBV.dial between PSV and waste1 @15:12:07.99 PSR.select :air @15:12:08.01 PSV.dial PSR @15:12:08.78 PS.reconfigure :fast @15:12:08.82 PS.seek 2.5ml @15:12:32.91 PSV.dial PTV @15:12:33.66 delay 5 seconds @15:12:38.68 PSV.dial PSR @15:12:39.91 delay 5 seconds @15:12:44.93 PSV.dial PTV @15:12:45.71 delay 5 seconds @15:12:50.73 PSV.dial :air @15:12:51.50 PS.seek 0ml @15:13:15.07 Gate.power :camera,:ON @15:13:15.08 Mixing DA Substrates @15:13:15.09 PSR.select :air @15:13:15.11 PSV.dial PSR @15:13:16.68 Pulling 0.25ml of PSR.sub1 @15:13:16.71 PSR.select :sub1 @15:13:16.90 PS.reconfigure :default @15:13:17.01 PS.seek 0.25ml @15:13:20.35 delay 5 seconds @15:13:25.42 Pulling 0.25ml of PSR.sub2 @15:13:25.43 PSR.select :sub2 @15:13:25.54 PS.seek 0.5ml @15:13:28.88 delay 5 seconds @15:13:33.90 PSR.select :air @15:13:34.01 PS.seek 2ml @15:13:50.19 PTV.dial :puck @15:13:50.20 PSV.dial PTV @15:13:50.96 PBV.dial :waste1 @15:13:51.39 PS.seek 1.5ml @15:13:57.29 delay 3 seconds @15:14:00.30 PBV.dial between PSV and waste1 @15:14:00.69 PS.seek 0ml @15:14:16.88 delay 10 seconds @15:14:26.91 recoverPSRreagent08 @15:14:26.92 PTV.dial :puck @15:14:26.93 PBV.dial :waste1 @15:14:27.29 delay 30 seconds @15:14:57.31 PBV.dial between PSV and waste1 @15:14:57.69 PSR.select :air @15:14:57.71 PSV.dial PSR @15:14:58.49 PS.reconfigure :fast @15:14:58.54 PS.seek 2.5ml @15:15:23.03 PSV.dial PTV @15:15:23.80 delay 5 seconds @15:15:28.82 PSV.dial PSR @15:15:29.58 delay 5 seconds @15:15:34.60 PSV.dial PTV @15:15:35.38 delay 5 seconds @15:15:40.40 PSV.dial :air @15:15:41.20 PS.seek 0ml @15:16:05.66 Additional air clear @15:16:05.67 PSR.select :air @15:16:05.69 PSV.dial PSR @15:16:07.10 Pulling 2ml of PSR.air @15:16:07.11 PSR.select :air @15:16:07.13 PS.reconfigure :default @15:16:07.17 PS.seek 2ml @15:16:28.38 delay 5 seconds @15:16:33.40 PTV.dial :puck @15:16:33.41 PSV.dial PTV @15:16:34.17 PBV.dial :waste1 @15:16:34.61 PS.seek 1.5ml @15:16:40.56 delay 3 seconds @15:16:43.58 PBV.dial between PSV and waste1 @15:16:43.97 delay 1 second @15:16:44.99 PSV.dial :waste1 @15:16:46.09 PS.seek 0ml @15:17:02.30 Processing Puck Heater at 27.9C before imaging @15:17:02.31 PH.coast @15:17:02.43 Imaging da puck in Processing Clamp while storing it to tube 1 @15:17:02.59 SC.reconfigure :tight @15:17:02.63 SC.seek 1 @15:17:06.20 SC.configure :limp @15:17:06.39 Elbow.reconfigure :tight @15:17:06.44 Elbow.seek 1 @15:17:08.25 Forearm.reconfigure :tight @15:17:08.29 Forearm.seek 1 + 450 counts @15:17:09.30 SE.reconfigure :pick @15:17:09.35 SE.seek :beamBreak @15:17:11.95 SE.configure :default @15:17:12.09 Elbow.reconfigure :limp @15:17:12.14 Forearm.reconfigure :loose @15:17:12.18 Forearm.seek 1 @15:17:12.39 Elbow.hold @15:17:12.46 Elbow.reconfigure :tight @15:17:12.55 SE.seek 9.11 Pucks below Top Plate @15:17:13.00 Forearm.force -20 @15:17:13.04 Forearm.seek 1 + 317 counts @15:17:13.31 Forearm.reconfigure :tight @15:17:13.35 Forearm.seek 1 + 450 counts @15:17:13.54 SE.seek 10.11 Pucks below Top Plate @15:17:14.14 Forearm.reconfigure :loose @15:17:14.18 Forearm.seek :retract @15:17:15.12 Elbow.seek PC @15:17:17.64 Elbow.reconfigure :loose @15:17:17.72 Forearm.seek PC @15:17:18.66 Hand.close @15:17:19.23 PC.seek :open @15:17:24.43 Forearm.seek :retract @15:17:25.51 Elbow.reconfigure :tight @15:17:25.55 Elbow.seek 1 @15:17:28.27 Forearm.reconfigure :tight @15:17:28.32 Forearm.seek 1 + 450 counts @15:17:29.28 Hand.open @15:17:29.91 SE.seek 9.76 Pucks below Top Plate @15:17:30.19 SE.reconfigure :seal @15:17:30.24 SE.seek 8.8 Pucks below Top Plate @15:17:30.75 Lights, camera, ACTION! @15:17:30.79 PuckCamera: snap -bin=2,2 -auto /var/log/esp/da21dec2715h100ml.tif @15:17:50.29 Waiting >20 seconds for da to relinquish FlushPuck @15:18:19.42 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 45.005 seconds /var/log/esp/da21dec2715h100ml.tif: TIFF Upload Complete @15:18:19.45 PuckCamera: snap -bin=2,2 15.0 /var/log/esp/midres/da21dec2715h100ml15s.tif @15:18:35.10 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 15 seconds /var/log/esp/midres/da21dec2715h100ml15s.tif: TIFF Upload Complete @15:18:35.13 PuckCamera: snap -bin=2,2 30.0 /var/log/esp/midres/da21dec2715h100ml30s.tif @15:19:05.82 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 696x520 pixel 16-bit image for 30 seconds /var/log/esp/midres/da21dec2715h100ml30s.tif: TIFF Upload Complete @15:19:05.85 PuckCamera: snap -bin=1,1 70.0 /var/log/esp/hires/da21dec2715h100ml70s.tif @15:20:17.70 SX HX9/16 (USB:02): 1392x1040 pixel 16-bit CCD camera Exposing 1392x1040 pixel 16-bit image for 70 seconds /var/log/esp/hires/da21dec2715h100ml70s.tif: TIFF Upload Complete @15:20:17.72 Gate.power :camera,:OFF @15:20:17.74 DAfans.email "45.005 sec auto-exposure",Subject: "da21dec2715h100ml images!" @15:20:18.01 Elbow.seek 1 @15:20:18.92 Forearm.seek 1 + 450 counts @15:20:18.99 SE.reconfigure :gently @15:20:19.04 SE.seek 9.51 Pucks below Top Plate @15:20:19.68 Elbow.reconfigure :limp @15:20:19.74 Forearm.reconfigure :loose @15:20:19.78 Forearm.seek 1 @15:20:20.00 Elbow.hold @15:20:20.07 Elbow.reconfigure :tight @15:20:20.14 SE.reconfigure :default @15:20:20.19 SE.seek 9.7 Pucks below Top Plate @15:20:20.54 Forearm.force -20 @15:20:20.58 Forearm.seek 1 + 306 counts @15:20:20.89 Forearm.reconfigure :tight @15:20:20.94 Forearm.seek 1 + 450 counts @15:20:21.70 SE.seek :down @15:20:22.83 Forearm.reconfigure :loose @15:20:22.88 Forearm.seek :retract @15:20:23.76 SE.stop @15:20:23.95 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @15:20:24.22 Elbow.seek FlushPuck::Garage @15:20:26.03 Elbow.reconfigure :loose @15:20:26.12 Forearm.seek :garage @15:20:27.16 Hand.close @15:20:28.13 Forearm.seek :retract @15:20:29.13 Elbow.reconfigure :tight @15:20:29.21 Elbow.seek PC @15:20:30.61 Elbow.reconfigure :loose @15:20:30.84 Forearm.reconfigure :tight @15:20:30.94 Forearm.seek PC @15:20:32.55 PC.seek :closed @15:20:37.40 Processing Clamp onSpring/offSpring for 14/25 samples, on/off torque 1.62 @15:20:37.52 Hand.open @15:20:38.24 Forearm.reconfigure :loose @15:20:38.32 Forearm.seek :retract @15:20:39.30 PSR.select :air @15:20:40.40 Storing wcr puck in Collection Clamp to tube 1 @15:20:40.48 PSV.dial PSR @15:20:41.45 Pulling 0.5ml of PSR.flush @15:20:41.51 PSR.select :flush @15:20:41.73 Elbow.reconfigure :tight @15:20:41.81 Elbow.seek 1 @15:20:41.93 PS.seek 0.5ml @15:20:44.65 Forearm.reconfigure :tight @15:20:44.75 Forearm.seek 1 + 450 counts @15:20:46.03 SE.reconfigure :pick @15:20:46.13 SE.seek :beamBreak @15:20:47.86 delay 5 seconds @15:20:48.74 SE.configure :default @15:20:49.05 Elbow.reconfigure :limp @15:20:49.13 Forearm.reconfigure :loose @15:20:49.21 Forearm.seek 1 @15:20:49.52 Elbow.hold @15:20:49.66 Elbow.reconfigure :tight @15:20:49.91 SE.seek 10.1 Pucks below Top Plate @15:20:50.54 Forearm.force -20 @15:20:50.58 Forearm.seek 1 + 295 counts @15:20:50.92 Forearm.reconfigure :tight @15:20:50.96 Forearm.seek 1 + 450 counts @15:20:51.18 SE.seek 11.1 Pucks below Top Plate @15:20:51.93 Forearm.reconfigure :loose @15:20:52.03 Forearm.seek :retract @15:20:52.94 PSR.select :air @15:20:53.04 Elbow.seek CC @15:20:53.22 PS.seek 2.5ml @15:20:54.84 Elbow.reconfigure :loose @15:20:55.04 Forearm.seek CC @15:20:56.05 Hand.close @15:20:56.77 CC.seek :open @15:21:01.89 Forearm.seek :retract @15:21:02.91 Elbow.reconfigure :tight @15:21:03.01 Elbow.seek 1 @15:21:04.98 Elbow.reconfigure :limp @15:21:05.08 Forearm.seek 1 @15:21:06.13 Elbow.hold @15:21:06.26 Elbow.reconfigure :tight @15:21:06.34 Hand.open @15:21:07.26 Forearm.force -20 @15:21:07.34 Forearm.seek 1 + 295 counts @15:21:07.84 Forearm.reconfigure :tight @15:21:07.92 Forearm.seek 1 + 450 counts @15:21:08.32 SE.seek :down @15:21:09.58 Forearm.reconfigure :loose @15:21:09.66 Forearm.seek :retract @15:21:10.36 SE.stop @15:21:10.68
Starting finalPurge @15:21:14.49 delay 1 second @15:21:15.54 PRV.dial :waste2,avoiding: :ab2 @15:21:17.45 PTV.dial :PRVmixing @15:21:18.83 PSV.dial :mixloop @15:21:20.03 PS.seek :empty @15:21:30.45
Waiting >20 seconds for psFlush to relinquish FlushPuck @15:21:40.12 upsync @15:21:46.46 PSR.select :air @15:21:46.48 PSV.dial PSR @15:21:47.58 Pulling 0.5ml of PSR.flush @15:21:47.59 PSR.select :flush @15:21:47.70 PS.seek 0.5ml @15:21:53.62 delay 5 seconds @15:21:58.64 PSR.select :air @15:21:58.75 PS.seek 2.5ml @15:22:20.64 delay 1 second @15:22:21.66 PBV.dial :waste1 @15:22:22.03 PTV.dial :mixing @15:22:22.78 PSV.dial :mixloop @15:22:23.84 PS.seek :empty @15:22:50.15 PSV.dial PSR @15:22:51.32 PSR.select :flush @15:22:51.44 PS.seek 1ml @15:23:02.68 PSR.select :air @15:23:02.79 PS.seek 2.5ml @15:23:18.96 PSV.dial PTV @15:23:19.71 PTV.dial :puck @15:23:20.46 PBV.dial :waste1 @15:23:20.49 PS.seek 0ml @15:23:46.90 PSV.dial PSR @15:23:47.67 PSR.select :flush @15:23:47.78 PS.seek 0.5ml @15:23:53.74 PSR.select :air @15:23:53.85 PS.seek 2.5ml @15:24:15.05 PSV.dial PTV @15:24:15.82 PBV.dial PSV @15:24:16.39 PS.seek 0ml @15:24:42.71 PSV.dial PBV @15:24:43.19 PS.seek 2.5ml @15:25:09.59 PSV.dial :waste1 @15:25:10.34 PS.seek 0ml @15:25:36.71 PSV.dial PSR @15:25:37.18 PSR.select :air @15:25:37.20 PS.seek 2.5ml @15:26:03.55 PSV.dial PTV @15:26:04.30 PBV.dial PSV @15:26:04.32 PS.seek 0ml @15:26:30.86 PSV.dial PBV @15:26:31.47 PS.seek 2.5ml @15:26:57.83 PSV.dial :waste1 @15:26:58.61 PS.seek 0ml @15:27:24.93 PSV.dial PSR @15:27:25.40 PSR.select :flush @15:27:25.51 PS.seek 1ml @15:27:36.52 PSR.select :air @15:27:36.63 PS.seek 2.5ml @15:27:52.83 PSV.dial PTV @15:27:53.55 PTV.dial :puck @15:27:53.57 PBV.dial :waste1 @15:27:54.12 PS.seek 0ml @15:28:20.49 PSV.dial PSR @15:28:21.25 PSR.select :flush @15:28:21.37 PS.seek 0.5ml @15:28:27.46 PSR.select :air @15:28:27.57 PS.seek 2.5ml @15:28:48.83 PSV.dial PTV @15:28:49.60 PBV.dial PSV @15:28:50.17 PS.seek 0ml @15:29:16.52 PSV.dial PBV @15:29:16.99 PS.seek 2.5ml @15:29:43.33 PSV.dial :waste1 @15:29:44.09 PS.seek 0ml @15:30:10.45 PSV.dial PSR @15:30:10.92 PSR.select :air @15:30:10.95 PS.seek 2.5ml @15:30:37.36 PSV.dial PTV @15:30:38.10 PBV.dial PSV @15:30:38.12 PS.seek 0ml @15:31:04.67 PSV.dial PBV @15:31:05.13 PS.seek 2.5ml @15:31:31.52 PSV.dial :waste1 @15:31:32.38 PS.seek 0ml @15:31:58.74
Loading FlushPuck into Collection Clamp from Processing Clamp @15:31:58.91 Elbow.seek PC @15:32:01.39 Elbow.reconfigure :loose @15:32:01.48 Forearm.seek PC @15:32:02.41 Hand.close @15:32:03.09 PC.seek :open @15:32:07.81 Forearm.seek :retract @15:32:08.76 Elbow.reconfigure :tight @15:32:08.80 Elbow.seek CC @15:32:12.42 Elbow.reconfigure :loose @15:32:12.51 Forearm.reconfigure :tight @15:32:12.55 Forearm.seek CC @15:32:14.28 CC.seek :closed @15:32:18.80 Collection Clamp onSpring/offSpring for 13/26 samples, on/off torque 2.32 @15:32:18.86 Hand.open @15:32:19.52 Forearm.reconfigure :loose @15:32:19.57 Forearm.seek :retract @15:32:20.53 Flushing the Collection Valve::Manifold @15:32:20.64 CSV.dial CSR @15:32:21.13 Pulling 5ml of CSR.flush @15:32:21.14 CSR.select :flush @15:32:21.26 CS.seek 5ml @15:32:35.08 delay 5 seconds @15:32:40.10 CSR.select :air @15:32:40.25 CS.reconfigure :fast @15:32:40.29 CS.seek 7.5ml @15:32:47.08 CSV.dial :waste @15:32:48.36 CS.reconfigure :default @15:32:48.41 CS.seek 0ml @15:33:08.33 Flushing lower manifold's waste1 port @15:33:08.44 CSV.dial :flush @15:33:08.90 CS.reconfigure :fast @15:33:08.94 CS.seek 5ml @15:33:21.83 delay 5 seconds @15:33:26.85 CSV.dial :air @15:33:27.36 CS.seek 7ml @15:33:32.95 CSV.dial CTV @15:33:32.97
CTV.dial CSV @15:33:34.39 CBV.dial :waste1 @15:33:35.15 CS.seek 0ml @15:33:51.95 Flushing lower manifold's waste2 port @15:33:52.07 CSV.dial :flush @15:33:52.82 CS.seek 5ml @15:34:05.77 delay 5 seconds @15:34:10.78 CSV.dial :air @15:34:11.31 CS.seek 7.001ml @15:34:16.86 CSV.dial CTV @15:34:16.91
CTV.dial CSV @15:34:17.33 CBV.dial :waste2 @15:34:18.40 CS.seek 0ml @15:34:35.11 Flushing lower manifold's waste2b port @15:34:35.23 CSV.dial :flush @15:34:36.00 CS.seek 5ml @15:34:48.69 delay 5 seconds @15:34:53.72 CSV.dial :air @15:34:54.27 CS.seek 7.001ml @15:34:59.86 CSV.dial CTV @15:34:59.91
CTV.dial CSV @15:35:00.33 CBV.dial :waste2b @15:35:01.42 CS.seek 0ml @15:35:18.23 Disinfecting external Intake with kill @15:35:18.35 CSV.dial CSR @15:35:18.96 Pulling 8ml of CSR.kill @15:35:18.97 CSR.select :kill @15:35:19.08 CS.seek 8ml @15:35:39.05 delay 5 seconds @15:35:44.06 CSR.select :air @15:35:44.18 CS.seek 10ml @15:35:49.83 CSV.dial CBV @15:35:49.85
CBV.dial CSV @15:35:50.94 CTV.dial :intake @15:35:51.72 Intake.select :open @15:35:51.82 CS.seek 2ml @15:36:10.98 Intake.select :close @15:36:11.08 CTV.dial :air @15:36:11.86 CS.reconfigure :default @15:36:11.90 CS.seek 0ml @15:36:17.89 CSV.dial CSR @15:36:18.96 Pulling 5ml of CSR.flush CSR.select :flush @15:36:19.07 CS.seek 5ml @15:36:32.86 delay 5 seconds @15:36:37.88 CSR.select :air @15:36:37.99 CS.seek 10ml @15:36:51.64 CSV.dial CBV @15:36:52.77 CS.seek 0ml @15:37:19.23 Garaging FlushPuck in Collection Clamp @15:37:19.30 Elbow.reconfigure :tight @15:37:19.34 Elbow.seek CC @15:37:19.73 Elbow.reconfigure :loose @15:37:19.82 Forearm.seek CC @15:37:20.80 Hand.close @15:37:21.46 CC.seek :open @15:37:26.09 Forearm.seek :retract @15:37:27.04 Elbow.reconfigure :tight @15:37:27.51 Elbow.seek :garage @15:37:30.48 Elbow.reconfigure :loose @15:37:30.72 Forearm.reconfigure :tight @15:37:30.77 Forearm.seek :garage @15:37:31.71 Hand.open @15:37:32.39 Forearm.reconfigure :loose @15:37:32.43 Forearm.seek :retract @15:37:33.37 Completed [initialPurge; da [100, 100] #da and wsr are run simultanelously] begun 13:43:13 @15:37:33.41 Asleep after completing DA_WCR_122621 assays @15:37:33.43 GoodNews.email "{1 => 12,\n 2 => 21,\n 3 => 33,\n 4 => 33,\n 5 => 33,\n 6 => 33,\n 7 => 31}\nCTD data unavailable\nCan@15:32:29, 18% flow, 16.5C, 36% humidity, 14.7psia, 13.593V, 0.514A, 0.496A avg, 6.99W\nSampled 100.0ml",Subject: "Asleep after completing DA_WCR_122621 assays" @15:38:08.58
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